Fibrin induces IL-8 expression from human oral squamous cell carcinoma cells.
In recent studies, we have demonstrated that fibrin is present in association with tumor cells in oral squamous cell carcinoma (OSCC) in vivo. We hypothesized that this fibrin can directly induce the expression of known angiogenic factors from oral tumor cells. Since IL-8 is known to be the major inducer of angiogenesis caused by these cells, we examined the ability of fibrin to stimulate IL-8 expression from OSCC cells in vitro. A physiologically relevant concentration of fibrin was found to cause a dose and time-dependent stimulation of IL-8 expression from oral and pharyngeal tumor cells but not from a non-tumorigenic oral cell line. Fibrinogen, thrombin and collagen were all unable to induce significant IL-8 expression, establishing the specificity of fibrin in causing this response. Gel filtration chromatography confirmed the molecular identity of the IL-8 antigen detected in the ELISA system used. These results suggest that fibrin may promote angiogenesis in oral tumors in vivo by directly upregulating the expression of IL-8 from tumor cells.
Lalla
R V
RV
Department of Oral Diagnosis, Division of Oral Medicine, University of Connecticut School of Dental Medicine, Farmington, CT 06030, USA.
Goralnick
S J
SJ
Tanzer
M L
ML
Kreutzer
D L
DL
D000704:Analysis of Variance; D002294:Carcinoma, Squamous Cell / Q000098:blood supply / Q000276:immunology*; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D004848:Epithelium; D005337:Fibrin / Q000494:pharmacology*; D006801:Humans; D016209:Interleukin-8 / Q000032:analysis / Q000378:metabolism*; D009061:Mouth Mucosa; D009062:Mouth Neoplasms / Q000098:blood supply / Q000276:immunology*; D009363:Neoplasm Proteins / Q000032:analysis / Q000378:metabolism*; D009389:Neovascularization, Pathologic; D010610:Pharyngeal Neoplasms / Q000098:blood supply / Q000276:immunology; D013268:Stimulation, Chemical; D013997:Time Factors; D014407:Tumor Cells, Cultured / Q000187:drug effects
D016209:Interleukin-8; D009363:Neoplasm Proteins; D005337:Fibrin