Molecular characterization of fibroblasts isolated from human peritoneum and adhesions.
OBJECTIVE
To determine the response of adhesion and peritoneal fibroblasts to hypoxia.


METHODS
Prospective experimental study.


METHODS
University medical center.


METHODS
Primary cultures of fibroblasts established from the peritoneal and adhesion tissues of the same patients (n = 2) to minimize genetic variations.


METHODS
Hypoxia treatment of the primary cultured fibroblast.


METHODS
Analyze the expression of extracellular matrix (ECM) components, metalloproteinases and their tissue inhibitors, growth factors, and cytokines in adhesion and peritoneal fibroblasts under normal and hypoxic conditions by reverse transcriptase/polymerase chain reaction analysis.


RESULTS
Compared to peritoneal fibroblasts, adhesion fibroblasts had a significant increase in the basal mRNA levels for collagen I, fibronectin, MMP-1, TIMP-1, TGF-beta 1, TGF-beta 2, and IL-10. Hypoxia resulted in a further increase in collagen 1, fibronectin, TIMP-1, TGF-beta 1, TGF-beta 2, IL-10, and IFN-gamma mRNA levels in both peritoneal and adhesion fibroblasts. The increase was more profound in adhesion fibroblasts.


CONCLUSIONS
Hypoxia induces molecular changes in both peritoneal and adhesion fibroblasts, creating a milieu that favors adhesion development. The effect of hypoxia was more profound on adhesion fibroblasts.
Saed
G M
GM
Department of Obstetrics and Gynecology, Hutzel Hospital, Wayne State University, Detroit, Michigan, USA. g.saed@wayne.edu
Zhang
W
W
Diamond
M P
MP
D015687:Cell Hypoxia; D002478:Cells, Cultured; D003094:Collagen / Q000235:genetics; D016326:Extracellular Matrix Proteins / Q000032:analysis / Q000235:genetics*; D005260:Female; D005347:Fibroblasts / Q000166:cytology* / Q000473:pathology / Q000502:physiology; D005353:Fibronectins / Q000235:genetics; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics; D016753:Interleukin-10 / Q000235:genetics; D010535:Laparoscopy; D020781:Matrix Metalloproteinase 1 / Q000235:genetics; D017699:Pelvic Pain / Q000473:pathology* / Q000601:surgery; D010537:Peritoneum / Q000166:cytology* / Q000473:pathology / Q000502:physiology; D012016:Reference Values; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000267:Tissue Adhesions / Q000473:pathology* / Q000503:physiopathology; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000235:genetics; D014158:Transcription, Genetic; D016212:Transforming Growth Factor beta / Q000235:genetics
D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D019715:Tissue Inhibitor of Metalloproteinase-1; D016212:Transforming Growth Factor beta; D016753:Interleukin-10; D007371:Interferon-gamma; D003094:Collagen; D020781:Matrix Metalloproteinase 1