title: Cerebrospinal fluid biogenic amine metabolites, plasma-rich platelet serotonin and [3H]imipramine reuptake in the primary fibromyalgia syndrome.
abstract: BACKGROUND
  Primary fibromyalgia syndrome (PFS) is a chronic disorder commonly seen in rheumatological practice. The pathophysiological disturbances of this syndrome, which was defined by the American College of Rheumatology in 1990, are poorly understood. This study evaluated, in 30 patients, the hypothesis that PFS is a pain modulation disorder induced by deregulation of serotonin metabolism.
  
  
  OBJECTIVE
  To compare platelet [(3)H]imipramine binding sites and serotonin (5-HT) levels in plasma-rich platelets (PRP) of PFS patients with those of matched healthy controls and to compare the levels of biogenic amine metabolites in the cerebrospinal fluid (CSF) of PFS patients with those of matched controls.
  
  
  METHODS
  Platelet [(3)H]imipramine binding sites were defined by two criteria, B(max) for their density and K(d) for their affinity. PRP 5-HT and CSF metabolites of 5-HT (5-hydroxyindoleacetic acid, 5-HIAA), norepinephrine (3-methoxy, 4-hydroxy phenylglycol, MHPG) and dopamine (homovanillic acid, HVA) were assayed by reversed-phase high-performance liquid chromatography with coulometric detection.
  
  
  RESULTS
  [(3)H]Imipramine platelet binding was similar (P=0.43 for B(max) and P=0.30 for K(d)) in PFS patients (B(max)=901+/-83 fmol/mg protein, K(d)=0.682+/-0.046) and in matched controls (B(max)=1017+/-119 fmol/mg protein, K(d)=0.606+/-0.056). PRP 5-HT was significantly higher (P=0.0009) in PFS patients (955+/-101 ng/10(9) platelets) than in controls (633+/-50 ng/10(9) platelets). When adjusted for age, the levels of all CSF metabolites were lower in PFS patients. The CSF metabolite of norepinephrine (MHPG) was lower (P:=0.003) in PFS patients (8.33+/-0.33 ng/ml) than in matched controls (9.89+/-0.31 ng/ml) and 5-HIAA was lower (P=0.042) in PFS female patients (22.34+/-1.78 ng/ml) than in matched controls (25.75+/-1.75 ng/ml). For HVA in females, the difference between PFS patients (36.32+/-3.20 ng/ml) and matched controls (38.32+/-2.90 ng/ml) approached statistical significance (P=0.054).
  
  
  CONCLUSIONS
  Changes in metabolites of CSF biogenic amines appear to be partially correlated to age but remained diagnosis-dependent. High levels of PRP 5-HT in PFS patients were associated with low CSF 5-HIAA levels in female patients but were not accompanied by any change in serotonergic uptake as assessed by platelet [(3)H]imipramine binding sites. These findings do not allow us to confirm that serotonin metabolism is deregulated in PFS patients.
authors: Department of Psychiatry, University of Caen, France.
authors: E
authors: E
authors: Legangneux
authors: J J
authors: JJ
authors: Mora
authors: O
authors: O
authors: Spreux-Varoquaux
authors: I
authors: I
authors: Thorin
authors: M
authors: M
authors: Herrou
authors: G
authors: G
authors: Alvado
authors: C
authors: C
authors: Gomeni
mesh_terms: D001679:Biogenic Amines / Q000134:cerebrospinal fluid*; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D004298:Dopamine / Q000378:metabolism; D005260:Female; D005356:Fibromyalgia / Q000097:blood* / Q000134:cerebrospinal fluid* / Q000503:physiopathology; D006801:Humans; D007099:Imipramine / Q000493:pharmacokinetics*; D008297:Male; D008875:Middle Aged; D009638:Norepinephrine / Q000378:metabolism; D012701:Serotonin / Q000378:metabolism*; D014316:Tritium / Q000493:pharmacokinetics
chemical_list: D001679:Biogenic Amines; D014316:Tritium; D012701:Serotonin; D007099:Imipramine; D004298:Dopamine; D009638:Norepinephrine