title: Potent anti-inflammatory activities of hydroalcoholic extract from aerial parts of Stachys inflata on rats.
abstract: Extracts obtained from aerial parts of Stachys inflata have been used in Iranian folk medicine in infective, rheumatic and other inflammatory disorders. In the present study, the anti-inflammatory properties of total methanol extract isolated from aerial parts of Stachys inflata were investigated in two well-characterised inflammatory models in rats, carrageenan-induced paw oedema and formalin-induced paw licking. Intraperitoneal injection of the extract, 60 min before induction of inflammation, revealed a dose-related inhibition of carrageenan-induced rat paw oedema over the dose range 50-200 mg/kg. In the formalin test, the extract (50, 100 and 200 mg/kg) had no effect against the first phase (0-5 min) of the formalin-induced pain, but all three doses produced a significant blockade of the second phase (P < 0.001). Myeloperoxidase (MPO) activity was determined, and a histopathological study was carried out in paw tissue 4 h after induction of inflammation. The hydroalcoholic extract (200 mg/kg) substantially reduced MPO activity (P < 0.05), which was increased in the control group. Histological examination showed a marked reduction in tissue injury and inhibition in neutrophil infiltration in rats treated with the extract (200 mg/kg).
authors: Department of Pharmacology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
authors: N
authors: N
authors: Maleki
authors: A
authors: A
authors: Garjani
authors: H
authors: H
authors: Nazemiyeh
authors: N
authors: N
authors: Nilfouroushan
authors: A T
authors: AT
authors: Eftekhar Sadat
authors: Z
authors: Z
authors: Allameh
authors: N
authors: N
authors: Hasannia
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D002351:Carrageenan / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D004487:Edema / Q000139:chemically induced / Q000517:prevention & control; D005557:Formaldehyde / Q000633:toxicity; D019686:Lamiaceae / Q000737:chemistry*; D008297:Male; D000432:Methanol / Q000737:chemistry; D010146:Pain / Q000139:chemically induced / Q000517:prevention & control; D009195:Peroxidase / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D010936:Plant Extracts; D005557:Formaldehyde; D002351:Carrageenan; D009195:Peroxidase; D000432:Methanol