title: Resistance to Lyme disease in decorin-deficient mice.
abstract: Microbial adhesion to the host tissue represents an early, critical step in the pathogenesis of most infectious diseases. BORRELIA: burgdorferi, the causative agent of Lyme disease (LD), expresses two surface-exposed decorin-binding adhesins, DbpA and DbpB. A decorin-deficient (Dcn(-/-)) mouse was recently developed and found to have a relatively mild phenotype. We have now examined the process of experimental LD in Dcn(-/-) mice using both needle inoculation and tick transmission of spirochetes. When exposed to low doses of the infective agent, Dcn(-/-) mice had fewer Borrelia-positive cultures from most tissues analyzed than did Dcn(+/+) or Dcn(+/-) mice. When the infection dose was increased, similar differences were not observed in most tissues but were seen in bacterial colonization of joints and the extent of Borreila-induced arthritis. Quantitative PCR demonstrated that joints harvested from Dcn(-/-) mice had diminished Borrelia numbers compared with issues harvested from Dcn(+/+) controls. Histological examination also revealed a low incidence and severity of arthritis in Dcn(-/-) mice. Conversely, no differences in the numbers of Borreila-positive skin cultures were observed among the different genotypes regardless of the infection dose. These differences, which were observed regardless of genetic background of the mice (BALB/c or C3H/HeN) or method of infection, demonstrate the importance of decorin in the pathogenesis of LD.
authors: The Center for Extracellular Matrix Biology, Texas A&M University System Health Science Center, Albert B. Alkek Institute of Biosciences and Technology, Houston, Texas 77030, USA. ebrown@ibt.tamu.edu
authors: E L
authors: EL
authors: Brown
authors: R M
authors: RM
authors: Wooten
authors: B J
authors: BJ
authors: Johnson
authors: R V
authors: RV
authors: Iozzo
authors: A
authors: A
authors: Smith
authors: M C
authors: MC
authors: Dolan
authors: B P
authors: BP
authors: Guo
authors: J J
authors: JJ
authors: Weis
authors: M
authors: M
authors: Höök
mesh_terms: D000818:Animals; D015748:Borrelia burgdorferi Group / Q000276:immunology / Q000472:pathogenicity*; D058575:Decorin; D004195:Disease Models, Animal; D016326:Extracellular Matrix Proteins; D005260:Female; D007113:Immunity, Innate; D018884:Ixodes; D008193:Lyme Disease / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D018345:Mice, Knockout; D011509:Proteoglycans / Q000235:genetics / Q000276:immunology / Q000502:physiology*
chemical_list: C546879:Dcn protein, mouse; D058575:Decorin; D016326:Extracellular Matrix Proteins; D011509:Proteoglycans