The antioxidant enzyme quinone reductase is up-regulated in vivo following cerebral ischemia.
An astrocyte antioxidant enzyme, quinone reductase (QR), was studied in vivo to assess whether its activity was up-regulated following cerebral ischemia. Rats were given a unilateral focal cerebral infarct and regions of interest within the ischemic penumbra compared to the non-ischemic side for QR activity. At 7 days post-ischemia, QR activity was significantly up-regulated within cells of astrocyte morphology in the cortex (p = 0.007) and subcortical (p = 0.005) areas adjacent to the infarct. This enzyme activity peaked at 7 days but was still significantly up-regulated at 14 days. Up-regulation of QR activity occurs within the ischemic penumbra of a stroke in this animal model and may contribute to factors that limit ischemic damage to neurons in this area.
Laxton
A W
AW
Department of Psychiatry, University of British Columbia, Vancouver, Canada.
Sun
M C
MC
Shen
H
H
Murphy
T H
TH
Honey
C R
CR
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D001253:Astrocytes / Q000502:physiology; D002545:Brain Ischemia / Q000201:enzymology*; D006651:Histocytochemistry; D008297:Male; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D015854:Up-Regulation / Q000502:physiology*
D000975:Antioxidants; D016660:NAD(P)H Dehydrogenase (Quinone)