title: Effects of liposome-mediated gene transfer of VEGF in ischemic rat gracilis muscle.
abstract: The purpose of the current study was to determine the effects of vascular endothelial growth factor (VEGF) on muscle flap survival and vascularity in a rat gracilis ischemia-reperfusion model. A total of 12 adult male Wistar rats were divided into two groups (n = 6). The experimental group received the plasmid encoding VEGF(165) cDNA plus lipofectamine (cationic liposome) injected directly to the gracilis muscle following 4 h of ischemia. The control group received lipofectamine only. The viability and vascularity of the flaps were evaluated after 7 days of reperfusion. The data demonstrated that the VEGF plasmid- and lipofectamine-treated muscle flaps had significantly greater total survival and capillary count 7 days after reperfusion compared with the flaps treated only with lipofectamine. These results indicate that VEGF exerts a protective effect on ischemic skeletal muscle flaps.
authors: Institute for Plastic and Reconstructive Surgery, Southern Illinois University, School of Medicine, Springfield, Illinois 62794-9653, USA. mneumeister@siumed.edu
authors: M W
authors: MW
authors: Neumeister
authors: Y H
authors: YH
authors: Song
authors: A
authors: A
authors: Mowlavi
authors: H
authors: H
authors: Suchy
authors: A
authors: A
authors: Mathur
mesh_terms: D000818:Animals; D002411:Cation Exchange Resins; D003097:Collateral Circulation; D018076:DNA, Complementary / Q000235:genetics; D016228:Endothelial Growth Factors / Q000235:genetics*; D004926:Escherichia coli; D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D005822:Genetic Vectors; D006614:Hindlimb; D007511:Ischemia / Q000628:therapy*; D008055:Lipids; D008222:Lymphokines / Q000235:genetics*; D008297:Male; D008833:Microcirculation; D018482:Muscle, Skeletal / Q000098:blood supply*; D010957:Plasmids; D051381:Rats; D017208:Rats, Wistar; D013524:Surgical Flaps / Q000098:blood supply; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
chemical_list: D002411:Cation Exchange Resins; D018076:DNA, Complementary; D016228:Endothelial Growth Factors; D008055:Lipids; C086724:Lipofectamine; D008222:Lymphokines; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors