Production of monoclonal antibody against Scrippsiella trochoidea cysts and its application to analysis during cyst formation and enzyme-linked immunosorbent assay.
A monoclonal antibody was produced by the fusion of mouse myeloma cells with spleen cells from a mouse immunized with the cysts of Scrippsiella trochoidea, marine phytoplankton. Immunofluorescence microscopic observation showed that the antibody reacted with the spines on the cyst but not with the vegetative cells and cyst walls. An enzyme-linked immunosorbent assay could be used to measure the cysts in muddy bottom sediments using the purified antibody conjugated with horseradish peroxidase.
Okazaki
K
K
Department of Life Sciences, Faculty of Agriculture, Kagawa University, Miki, Japan. okazaki@ag.kagawa-u.ac.jp
Iwaoka
T
T
Murakami
N
N
Ichimi
K
K
Montani
S
S
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000913:Antibodies, Protozoan / Q000096:biosynthesis*; D000953:Antigens, Protozoan / Q000378:metabolism; D002452:Cell Count; D004141:Dinoflagellida / Q000254:growth & development* / Q000276:immunology* / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D019015:Geologic Sediments / Q000469:parasitology; D006735:Horseradish Peroxidase; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008856:Microscopy, Fluorescence
D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D006735:Horseradish Peroxidase