title: Recombinant derivatives of clostridial neurotoxins as delivery vehicles for proteins and small organic molecules.
abstract: Clostridial neurotoxins are the most powerful toxins known. Nevertheless, derivatives of these toxins may find broad applications both in science and medicine because of their unique abilities to recognize neurons and deliver small and large molecules into them. In this paper we describe the construction of two types of such derivatives. Proteins belonging to the first class were designed to allow direct conjugation with one or few molecules of interest. Proteins belonging to the second class contain biotin residue and therefore could be easily connected to streptavidin loaded with multiple molecules of interest. Only C-terminal regions of neurotoxin heavy chains were incorporated in the structure of recombinant proteins. Nevertheless, recombinant proteins were found to be able to recognize specific neuronal receptors and target model molecules to rat synaptosomes and human neuroblastoma cells.
authors: Promega Corporation, Madison, Wisconsin 53711-5399, USA.
authors: M V
authors: MV
authors: Zdanovskaia
authors: G
authors: G
authors: Los
authors: A G
authors: AG
authors: Zdanovsky
mesh_terms: D000818:Animals; D001483:Base Sequence; D001905:Botulinum Toxins / Q000008:administration & dosage* / Q000235:genetics; D017931:DNA Primers; D004337:Drug Carriers; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D009930:Organic Chemicals / Q000008:administration & dosage*; D011506:Proteins / Q000008:administration & dosage*; D051381:Rats; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000235:genetics; D014407:Tumor Cells, Cultured
chemical_list: D017931:DNA Primers; D004337:Drug Carriers; D009930:Organic Chemicals; D011506:Proteins; D011994:Recombinant Proteins; D001905:Botulinum Toxins