Prolongation of survival of rats injected with hepatoma cells treated by nuclei extracts from mouse and rat embryo cells.
Cancer cells express particular genes, part of which are normally active during the embryonic development. On the other hand, young embryos are able to differentiate teratocarcinoma or leukemia cells, likely by producing differentiation factors. In this work, rat and mouse embryo cell nuclei extracts were tested on hepatoma carcinoma cells LFCl2A: they inhibit the cell growth in culture and increase the survival of syngeneic rats injected with hepatoma cells incubated with these extracts. This inhibition is correlated with a decrease of DNA synthesis without toxic effect: It seems to be due to the mixture of tight binding DNA proteins, possibly transcriptional factors.
Berger
G
G
Section de Bioenergetique, Departement de Biologie Cellulaire et Moleculaire CEN Saclay, 91191 Gif/Yvette, France.
Frayssinet
C
C
Berger
M
M
Lafarge Frayssinet
C
C
D000818:Animals; D002454:Cell Differentiation; D002457:Cell Extracts / Q000494:pharmacology; D002467:Cell Nucleus / Q000502:physiology; D003470:Culture Media; D004247:DNA / Q000096:biosynthesis; D004261:DNA Replication; D004273:DNA, Neoplasm / Q000378:metabolism; D004622:Embryo, Mammalian / Q000502:physiology*; D005805:Genes, MHC Class I / Q000502:physiology; D006131:Growth Inhibitors / Q000032:analysis; D007124:Immunoenzyme Techniques; D008114:Liver Neoplasms, Experimental / Q000378:metabolism / Q000401:mortality / Q000517:prevention & control*; D051379:Mice; D051381:Rats; D015996:Survival Rate; D005723:gamma-Glutamyltransferase / Q000378:metabolism
D002457:Cell Extracts; D003470:Culture Media; D004273:DNA, Neoplasm; D006131:Growth Inhibitors; D004247:DNA; D005723:gamma-Glutamyltransferase