The evaluation of lipid peroxidation and adenosine deaminase activity in patients with Behçet's disease.
OBJECTIVE
Despite unknown etiology, immunologic alterations and neutrophil hyperfunctions may be involved in the etiopathogenesis of Behçet's Disease (BD). The purpose of the study was to investigate whether adenosine deaminase (ADA) activity, accepted as a nonspecific marker of T lymphocyte activation, may have a potential role in BD, and also may be related to the production of reactive oxygen species (ROS) by neutrophils.


METHODS
ADA activities and malondialdehyde (MDA; endproduct of lipid peroxidation induced by ROS) levels in both plasma and erythrocytes were spectrophotometrically measured in 25 patients with BD and also in 25 healthy controls.


RESULTS
ADA activity was found to be higher in plasma, but lower in erythrocytes; plasma and erythrocyte MDA levels were higher in BD patients than those of controls. In addition, plasma ADA activity was positively related to MDA levels in both plasma (p < 0.05) and erythrocytes (p < 0.01). There was also positive correlation between MDA levels (p < 0.05), but negative correlations between ADA activities (p < 0.01) and also between ADA and MDA values in erythrocytes (p < 0.01) of BD patients.


CONCLUSIONS
These findings may provide some evidence for a potential role of T lymphocyte activation in BD as reflected by increased plasma ADA activity, and for the presence of possible interrelationship between activated T cells and neutrophil hyperfunctions, such as ROS generation, as reflected by increased MDA levels.
Köse
K
K
Erciyes University, Faculty of Medicine, Department of Biochemistry, 38039, Kayseri, Turkey. yazici@erciyes.edu.tr
Yazici
C
C
Aşşioğlu
O
O
D000243:Adenosine Deaminase / Q000097:blood / Q000378:metabolism*; D000293:Adolescent; D000328:Adult; D001528:Behcet Syndrome / Q000097:blood / Q000378:metabolism*; D016022:Case-Control Studies; D004912:Erythrocytes / Q000201:enzymology; D005260:Female; D006801:Humans; D015227:Lipid Peroxidation; D008213:Lymphocyte Activation; D008297:Male; D008315:Malondialdehyde / Q000097:blood / Q000378:metabolism; D008875:Middle Aged; D009504:Neutrophils / Q000378:metabolism; D017382:Reactive Oxygen Species
D017382:Reactive Oxygen Species; D008315:Malondialdehyde; D000243:Adenosine Deaminase