FTIR study of horseradish peroxidase in reverse micelles.
Fourier transform infrared (FTIR) method was used to study the secondary structures of horseradish peroxidase (HRP) in aqueous solution and in reverse micelles for the first time. Results indicated that the structure of HRP in sodium bis(2-ethylhexy)sulfosuccinate (AOT) reverse micelles was close to that in aqueous solution. In cetyltrimethylammonium bromide (CTAB) and sodium dodecylfate (SDS) reverse micelles the position of some bands changed. Results indicated that the secondary structure had a close relationship with the surfactant species of the reverse micelles. Among the three types of reverse micelles, the system of AOT reverse micelles was probably the most beneficial reaction media to HRP.
Chen
J
J
State Key Laboratory for Oxo Synthesis and Selective Oxidation, Lanzhou Institute of Chemical Physics, Lanzhou, 730000, People's Republic of China.
Xia
C
C
Niu
J
J
Li
S
S
D017666:Deuterium Oxide / Q000737:chemistry; D004143:Dioctyl Sulfosuccinic Acid / Q000737:chemistry; D006735:Horseradish Peroxidase / Q000737:chemistry*; D006860:Hydrogen Bonding; D008567:Membranes, Artificial; D008823:Micelles; D017433:Protein Structure, Secondary / Q000502:physiology; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared
D008567:Membranes, Artificial; D008823:Micelles; D000644:Quaternary Ammonium Compounds; D004143:Dioctyl Sulfosuccinic Acid; C039795:trimethylethylammonium; D012967:Sodium Dodecyl Sulfate; D006735:Horseradish Peroxidase; D017666:Deuterium Oxide