Self-referencing ceramic-based multisite microelectrodes for the detection and elimination of interferences from the measurement of L-glutamate and other analytes.
A self-referencing technique utilizing two microelectrodes on a ceramic-based multisite array is employed for confirmation and elimination of interferences detected by enzyme-based microelectrodes. The measurement of L-glutamate using glutamate oxidase was the test system; however, other oxidase enzymes such as glucose oxidase can be employed. One recording site was coated with Nafion with L-glutamate oxidase and bovine serum albumin (BSA) cross-linked with glutaraldehyde while the other had Nafion with BSA cross-linked with glutaraldehyde. Differences in the chemistry of the two recording sites allowed for identification and elimination of interfering signals to be removed from the analyte response. The electrode showed low detection limits (LOD = 0.98 +/- 0.09 microM, signal-to-noise ratio of 3), fast response times (T90 approximately 1 s), and excellent linearity (R2 = 0.999 +/- 0.000) over the concentration range of 0-200 microM for calibrations of L-glutamate in vitro. The selectivity and dimensions of the multisite electrode allow in vivo glutamate measurements. This electrode has been applied to in vivo measurements of the clearance of locally applied glutamate and release of glutamate in the prefrontal cortex of anesthetized rats. In addition, a aimilar approach has been applied to the development of a microelectrode for measures of glucose.
Burmeister
J J
JJ
Department of Anatomy, Center for Sensor Technology, University of Kentucky, Lexington 40536-0098, USA.
Gerhardt
G A
GA
D000594:Amino Acid Oxidoreductases; D000818:Animals; D001923:Brain Chemistry; D002516:Ceramics; D004800:Enzymes, Immobilized; D005947:Glucose / Q000032:analysis; D005949:Glucose Oxidase; D018698:Glutamic Acid / Q000032:analysis* / Q000378:metabolism; D007202:Indicators and Reagents; D008839:Microelectrodes; D017397:Prefrontal Cortex / Q000737:chemistry / Q000378:metabolism; D051381:Rats; D011916:Rats, Inbred F344; D012710:Serum Albumin, Bovine
D004800:Enzymes, Immobilized; D007202:Indicators and Reagents; D012710:Serum Albumin, Bovine; D018698:Glutamic Acid; D005949:Glucose Oxidase; D000594:Amino Acid Oxidoreductases; C023061:L-glutamate oxidase; D005947:Glucose