title: Influence of infection of cells with bacteria associated with reactive arthritis on the peptide repertoire presented by HLA-B27.
abstract: Reactive arthritis (ReA) after infections with various gram-negative bacteria is strongly associated with the MHC class I molecule HLA-B27. It is supposed that the B27 molecule itself plays a role in the pathogenesis of ReA by presenting antigenic peptides to cytotoxic T lymphocytes. The peptide repertoires presented by Salmonella-, Shigella- and non-infected cells were compared to identify such peptides. From the peptides isolated from the B27 molecules of these cells, profiles were generated by reversed-phase chromatography and peaks present in the profiles from infected cells but not in profiles from non-infected cells were studied for their peptide compositions. Some sequences with identity to those in human histone H3, human ribosomal protein S17 and the heavy chain of HLA-B27 itself were detected only in profiles from infected cells. All peptides identified from infected cells contained the B*2705 peptide-binding motif. The data suggest that HLA-B27-positive cells infected with ReA-inducing bacteria show an increased presentation of certain self-peptides. There was no evidence for altered peptide-binding specificity of B27 after infection. However, the interpretations were hampered by the variation in peptide presentation between different experiments.
authors: Jeffrey H
authors: JH
authors: Ringrose
authors: Department of Medical Microbiology, *E.C. Slater Institute and Department of Biochemistry, Academic Medical Center, University of Amsterdam, †Department of Transplantation Immunology, Central Laboratory of the Blood Transfusion Service, Amsterdam and ‡Arthron, Amsterdam, The Netherlands.
authors: Anton O
authors: AO
authors: Muijsers
authors: Yvonne
authors: Y
authors: Pannekoek
authors: Department of Medical Microbiology, *E.C. Slater Institute and Department of Biochemistry, Academic Medical Center, University of Amsterdam, †Department of Transplantation Immunology, Central Laboratory of the Blood Transfusion Service, Amsterdam and ‡Arthron, Amsterdam, The Netherlands.
authors: Benito A
authors: BA
authors: Yard
authors: Department of Medical Microbiology, *E.C. Slater Institute and Department of Biochemistry, Academic Medical Center, University of Amsterdam, †Department of Transplantation Immunology, Central Laboratory of the Blood Transfusion Service, Amsterdam and ‡Arthron, Amsterdam, The Netherlands.
authors: Claire J P
authors: CJP
authors: Boog
authors: Loek VAN
authors: LV
authors: Alphen
authors: Jacob
authors: J
authors: Dankert
authors: Department of Medical Microbiology, *E.C. Slater Institute and Department of Biochemistry, Academic Medical Center, University of Amsterdam, †Department of Transplantation Immunology, Central Laboratory of the Blood Transfusion Service, Amsterdam and ‡Arthron, Amsterdam, The Netherlands.
authors: Theodorus E W
authors: TEW
authors: Feltkamp
mesh_terms: D017951:Antigen Presentation; D016918:Arthritis, Reactive / Q000209:etiology*; D002478:Cells, Cultured; D004405:Dysentery, Bacillary / Q000150:complications / Q000382:microbiology; D015796:HLA-B27 Antigen / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification; D006801:Humans; D010455:Peptides / Q000737:chemistry / Q000276:immunology*; D000091402:Prohibitins; D012480:Salmonella Infections / Q000150:complications / Q000382:microbiology; D012486:Salmonella typhimurium / Q000276:immunology / Q000502:physiology*; D012763:Shigella flexneri / Q000276:immunology / Q000502:physiology*
chemical_list: D015796:HLA-B27 Antigen; C000717331:PHB2 protein, human; D010455:Peptides; D000091402:Prohibitins