title: Transmembrane signaling through phospholipase C in cortical and hippocampal membranes of psychogenetically selected rat lines.
abstract: BACKGROUND
  One of the major pathways for neurotransmitter signaling involves phosphoinositide-specific and G-protein-dependent phospholipase C-beta (PLC-beta), which stimulates the formation of inositol 1,4,5-trisphosphate and diacylglycerol. Serotonergic and muscarinic-cholinergic signals in the brain are largely mediated through the hydrolysis of phosphoinositides by PLC.
  
  
  OBJECTIVE
  The aim of the experiments reported here was to explore the potential differences in neurotransmitter receptor coupling to PLC in Roman high-avoidance (RHA)/Verh and Roman low-avoidance (RLA)/Verh rats, by examining the changes in agonist (carbachol, 5-methyltryptamine)-stimulated phosphoinositide hydrolysis in hippocampal and cortical membranes derived from the two rat lines.
  
  
  METHODS
  To investigate changes in receptor and G-protein coupling to PLC in the brains of these two psychogenetically selected rat lines, which differ in their emotional profiles/learning abilities, we examined GTPgammaS-, agonist (carbachol, 5-methyltryptamine)-, and calcium-stimulated phosphoinositide hydrolysis in cortical and hippocampal membranes of RHA/Verh and RLA/Verh rats.
  
  
  RESULTS
  The results indicated that calcium-induced increase in PLC activity was larger in the cortex and hippocampus of RHA/Ver rats, as compared to their RLA/Verh counterparts. Conversely, GTPgammaS- and agonist-induced PLC activity was less pronounced in the hippocampus of RHA/Verh with respect to RLA/Verh rats. Western blot analysis showed no significant differences in the relative values of the G-proteins alphaq/11 and betagamma subunits between both groups of rats in any brain region. However, the levels of PLC-beta1, PLC-beta3, and PLC-beta4 were significantly lower in the hippocampus of RHA/Verh than in RLA/Verh rats.
  
  
  CONCLUSIONS
  It is concluded that the hippocampus of RHA/Verh rats has severe deficiencies in PLC activity stimulated by guanine nucleotides and agonists, which are specifically related to a lower level of expression of the PLC-beta type isozymes, a fact that may account for the differential behavioral phenotype observed in these psychogenetically selected rat lines.
authors: Departamento de Farmacología, Facultad de Farmacia, Universidad del País Vasco/EHU, Victoria-Gasteiz, Alava, Spain. kfpsaalj@vc.ehu.es
authors: J
authors: J
authors: Sallés
authors: M
authors: M
authors: López de Jesús
authors: O
authors: O
authors: Goñi
authors: A
authors: A
authors: Fernández-Teruel
authors: P
authors: P
authors: Driscoll
authors: A
authors: A
authors: Tobeña
authors: R M
authors: RM
authors: Escorihuela
mesh_terms: D000818:Animals; D002217:Carbachol / Q000494:pharmacology; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism*; D018679:Cholinergic Agonists / Q000494:pharmacology; D019204:GTP-Binding Proteins / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D005824:Genetics, Behavioral; D016244:Guanosine 5'-O-(3-Thiotriphosphate) / Q000494:pharmacology; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism*; D007527:Isoenzymes / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D008297:Male; D019269:Phosphatidylinositol 4,5-Diphosphate / Q000235:genetics / Q000378:metabolism*; D054799:Phospholipase C beta; D051381:Rats; D015398:Signal Transduction; D013045:Species Specificity; D014363:Tryptamines / Q000494:pharmacology; D010738:Type C Phospholipases / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*
chemical_list: D018679:Cholinergic Agonists; D007527:Isoenzymes; D019269:Phosphatidylinositol 4,5-Diphosphate; D014363:Tryptamines; C027552:5-methyltryptamine; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D002217:Carbachol; D010738:Type C Phospholipases; D054799:Phospholipase C beta; C518035:Plcb1 protein, rat; C518041:Plcb3 protein, rat; C518044:Plcb4 protein, rat; D019204:GTP-Binding Proteins