title: Hematopoietic progenitor cell counts performed by the Sysmex SE-9000 analyzer can guide timing of peripheral blood stem cell harvest.
abstract: BACKGROUND
  We studied whether the hematopoietic progenitor cell (HPC) count in peripheral blood as evaluated by an automated counter, the Sysmex SE-9000, correlated with CD34 positive (+) cell count and therefore could guide the timing of peripheral blood stem cell (PBSC) harvest.
  
  
  METHODS
  HPC count and flow cytometric CD34+ cell count were measured in 90 peripheral blood samples and 30PBSC samples. The correlation between HPC count and apheretic CD34+ cell yield was examined in 19 patients.
  
  
  RESULTS
  HPC count showed good correlations with CD34+ cell count in peripheral blood (r = 0.699) and PBSC (r = 0.892). The correlation between peripheral blood HPC count and apheretic CD34+ cell yield also was good (r = 0.789).
  
  
  CONCLUSIONS
  Automated HPC counting can be used as a screening test to guide the timing of PBSC harvest.
authors: Division of Laboratory Diagnosis, Sapporo Medical University School of Medicine, South-1, West-16, Chuo-ku, Sapporo 060-8543, Japan.
authors: A
authors: A
authors: Endoh
authors: A
authors: A
authors: Yagihashi
authors: K
authors: K
authors: Asanuma
authors: R
authors: R
authors: Moriai
authors: A
authors: A
authors: Izawa
authors: Y
authors: Y
authors: Koyanagi
authors: T
authors: T
authors: Sato
authors: D
authors: D
authors: Kobayashi
authors: N
authors: N
authors: Watanabe
mesh_terms: D000328:Adult; D018952:Antigens, CD34 / Q000032:analysis; D001772:Blood Cell Count / Q000295:instrumentation*; D001781:Blood Component Removal / Q000379:methods*; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology; D019650:Hematopoietic Stem Cell Mobilization; D006412:Hematopoietic Stem Cells; D006801:Humans; D008297:Male; D013584:Sarcoma, Synovial / Q000097:blood; D013997:Time Factors; D014594:Uterine Neoplasms / Q000097:blood
chemical_list: D018952:Antigens, CD34; D016179:Granulocyte Colony-Stimulating Factor