title: Epinephrine causes a reduction in lymph node cell output in sheep.
abstract: The lymphatic system has a critical role in the return of fluids, proteins, and cells to the circulatory system. However, the effects of stress, including exercise, on this system have not been adequately studied. We investigated the effect of a physiological dose (1 mg) of epinephrine (Epi) on lymph flow, cell concentration, and lymphocyte subsets in efferent subcutaneous lymph in sheep. Blood leukocyte numbers, differential, lymphocyte subsets, and blood and lymph pools of lymphocytes were determined simultaneously. A significant acute increase in lymph flow was followed by a post-injection decrease in flow and cellular output. No changes in lymphocyte subsets or pools of lymphocytes were seen in either blood or lymph. The timing of elevated plasma and lymph concentrations of Epi and norepinephrine (NE) corresponded with the increased lymph flow. In conclusion, Epi injection caused no change in lymphocyte subset distribution, leukocyte concentration, or pools of lymphocytes. A decrease in lymph flow and cellularity was documented post-injection, indicating that lymphatic tissue has no role in the leukocytosis seen after Epi injection. Lymphocyte retention by lymph nodes, however, may contribute to post-injection lymphopenia.
authors: Department of Laboratory Medicine and Pathobiology, University of Toronto, Ontario, Canada. jack.hay@utoronto.ca
authors: T J
authors: TJ
authors: Seabrook
authors: B
authors: B
authors: Ristevski
authors: S G
authors: SG
authors: Rhind
authors: P N
authors: PN
authors: Shek
authors: J
authors: J
authors: Zamecnik
authors: R J
authors: RJ
authors: Shephard
authors: J B
authors: JB
authors: Hay
mesh_terms: D000316:Adrenergic alpha-Agonists / Q000494:pharmacology*; D000818:Animals; D004837:Epinephrine / Q000494:pharmacology*; D008198:Lymph Nodes / Q000166:cytology* / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects*; D009504:Neutrophils / Q000187:drug effects; D009638:Norepinephrine / Q000494:pharmacology; D010641:Phenotype; D012756:Sheep / Q000502:physiology*
chemical_list: D000316:Adrenergic alpha-Agonists; D009638:Norepinephrine; D004837:Epinephrine