P13, an integral membrane protein of Borrelia burgdorferi, is C-terminally processed and contains surface-exposed domains.
To elucidate antigens present on the bacterial surface of Borrelia burgdorferi sensu lato that may be involved in pathogenesis, we characterized a protein, P13, with an apparent molecular mass of 13 kDa. The protein was immunogenic and was expressed in large amounts during in vitro cultivation compared to other known antigens. An immunofluorescence assay, immunoelectron microscopy, and protease sensitivity assays indicated that P13 is surface exposed. The deduced sequence of the P13 peptide revealed a possible signal peptidase type I cleavage site, and computer analysis predicted that P13 is an integral membrane protein with three transmembrane-spanning domains. Mass spectrometry, in vitro translation, and N- and C-terminal amino acid sequencing analyses indicated that P13 was posttranslationally processed at both ends and modified by an unknown mechanism. Furthermore, p13 belongs to a gene family with five additional members in B. burgdorferi sensu stricto. The p13 gene is located on the linear chromosome of the bacterium, in contrast to five paralogous genes, which are located on extrachromosomal plasmids. The size of the p13 transcript was consistent with a monocistronic transcript. This new gene family may be involved in functions that are specific for this spirochete and its pathogenesis.
Noppa
L
L
Department of Microbiology, Umeå University, SE-901 87 Umeå, Sweden.
Ostberg
Y
Y
Lavrinovicha
M
M
Bergström
S
S
D000595:Amino Acid Sequence; D000942:Antigens, Bacterial / Q000032:analysis / Q000737:chemistry* / Q000235:genetics; D000954:Antigens, Surface / Q000032:analysis / Q000737:chemistry; D001483:Base Sequence; D015748:Borrelia burgdorferi Group / Q000276:immunology*; D003001:Cloning, Molecular; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011499:Protein Processing, Post-Translational; D012333:RNA, Messenger / Q000032:analysis
D000942:Antigens, Bacterial; D000954:Antigens, Surface; D012333:RNA, Messenger