Molecular and genomic analysis of genes encoding surface-anchored proteins from Clostridium difficile.
The gene slpA, encoding the S-layer precursor protein in the virulent Clostridium difficile strains C253 and 79--685, was identified. The precursor protein carries a C-terminal highly conserved anchoring domain, similar to the one found in the Cwp66 adhesin (previously characterized in strain 79--685), an SLH domain, and a variable N-terminal domain mediating cell adherence. The genes encoding the S-layer precursor proteins and the Cwp66 adhesin are present in a genetic locus carrying 17 open reading frames, 11 of which encode a similar two-domain architecture, likely to include surface-anchored proteins.
Karjalainen
T
T
Département de Microbiologie, Faculté de Pharmacie, Université de Paris-Sud, 92296 Châtenay-Malabry Cedex, France.
Waligora-Dupriet
A J
AJ
Cerquetti
M
M
Spigaglia
P
P
Maggioni
A
A
Mauri
P
P
Mastrantonio
P
P
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics*; D016360:Clostridioides difficile / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family; D016366:Open Reading Frames; D017385:Sequence Homology
D001426:Bacterial Proteins; C070683:surface layer protein A, Bacteria