Nitrification inhibitors from the roots of Leucaena leucocephala.
The nitrification inhibition (NI) bioassay guided fractionation of the methanol extract of lyophilized and milled roots of Leuceana leucocephala resulted in the isolation of four compounds, 1-4, as confirmed from their 1H and 13C NMR spectral data. Compound 1, gallocatechin, was the most active NI inhibitor at 12 microg/mL. Epigallocatechin, 2, and epicatechin, 4, isolated as mixtures, were not assayed individually for their NI inhibitory activities against the nitrification bacterium Nitrosomonas europaea.
Erickson
A J
AJ
Department of Horticulture and National Food Safety and Toxicology Center and Department of Crop and Soil Sciences, Michigan State University, East Lansing, Michigan 48824, USA.
Ramsewak
R S
RS
Smucker
A J
AJ
Nair
M G
MG
D002392:Catechin / Q000031:analogs & derivatives / Q000032:analysis; D007887:Fabaceae / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D009584:Nitrogen / Q000037:antagonists & inhibitors* / Q000378:metabolism; D009606:Nitrosomonas / Q000378:metabolism*; D010946:Plants, Medicinal
D002392:Catechin; C057580:gallocatechol; D009584:Nitrogen