Bacillomycin D: an iturin with antifungal activity against Aspergillus flavus.
OBJECTIVE
In a search for an antifungal peptide with a high activity against Aspergillus flavus, Bacillus subtilis AU195 was selected from a collection of isolates with antagonistic activity against A. flavus.


RESULTS
To identify the antifungal peptides, a protein purification scheme was developed based on the detection of the antifungal activity in purified fractions against A. flavus. Two lipopeptides were purified with anion exchange and gel filtration chromatography. Their masses were determined to be 1045 and 1059 m/z with mass spectrometry, and their peptide moiety was identical to bacillomycin D.


CONCLUSIONS
AU195 synthesized a mixture of two antifungal bacillomycin D analogues with masses of 1045 and 1059, the 14 mass unit difference representing the difference between a C15 and a C16 lipid chain.


CONCLUSIONS
Both bacillomycin D analogues were active at the same concentration against A. flavus, but the different lipid chain length apparently affected the activity of the lipopeptide against other fungi.
Moyne
A L
AL
Department of Entomology and Plant Pathology, 209 Life Sciences Building, Auburn University, Auburn, AL 36849, USA. amoyne@acesag.auburn.edu
Shelby
R
R
Cleveland
T E
TE
Tuzun
S
S
D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides; D001231:Aspergillus flavus / Q000187:drug effects*; D001412:Bacillus subtilis / Q000737:chemistry*; D002850:Chromatography, Gel / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D008826:Microbial Sensitivity Tests; D010455:Peptides; D020539:Sequence Analysis, Protein
D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D023181:Antimicrobial Cationic Peptides; D010455:Peptides; C027845:bacillomycin D