Surfactant proteins D and A in sputum.
The surfactant proteins SP-D and SP-A are collectins and central components of the innate immune system of the peripheral lungs. They bind to carbohydrates on microorganisms and promote their removal. In the larger airways their role and concentrations are not yet known. Sputum may thus potentially be useful to characterize the collectins in this compartment. Induced sputum was obtained from healthy adults and spontaneous sputum from patients with cystic fibrosis. ELISA and carbohydrate binding assays were used to quantify the amount and functional capacity of the collectins in plugs or sol-fractions, prepared by centrifugation of the plugs. SP-D and SP-A were detectable in only about 50% of the sputum plugs. Varying the salt, detergent and calcium conditions of the assay did not improve the results. In samples with reproducibly detectable SP-D or SP-A, their carbohydrate binding capacity was zero. Sputum sol fraction and LPS inhibited the binding of the collectins to carbohydrate in the presence of calcium. Whereas SP-D and SP-A are abundant in the peripheral lung, their presence in sputum derived from the larger airways is variable and their carbohydrate binding capacity is lost.
Griese
M
M
Kinderpoliklinik, Dr. von Hauner Children's Hospital, Ludwig-Maximilians-University, Pettenkoferstr 8a, D-80336 Munich, Germany. griese@pk-i.med.uni-muenchen.de
Maderlechner
N
N
Bufler
P
P
D000293:Adolescent; D000328:Adult; D050260:Carbohydrate Metabolism; D002614:Chelating Agents; D002648:Child; D003550:Cystic Fibrosis / Q000378:metabolism*; D003902:Detergents; D004492:Edetic Acid; D004797:Enzyme-Linked Immunosorbent Assay; D006023:Glycoproteins / Q000032:analysis*; D006801:Humans; D008070:Lipopolysaccharides; D008168:Lung / Q000737:chemistry* / Q000502:physiology; D008875:Middle Aged; D017830:Octoxynol; D011510:Proteolipids / Q000032:analysis*; D037662:Pulmonary Surfactant-Associated Protein A; D037663:Pulmonary Surfactant-Associated Protein D; D037661:Pulmonary Surfactant-Associated Proteins; D011663:Pulmonary Surfactants / Q000032:analysis*; D012995:Solubility; D013183:Sputum / Q000737:chemistry*
D002614:Chelating Agents; D003902:Detergents; D006023:Glycoproteins; D008070:Lipopolysaccharides; D011510:Proteolipids; D037662:Pulmonary Surfactant-Associated Protein A; D037663:Pulmonary Surfactant-Associated Protein D; D037661:Pulmonary Surfactant-Associated Proteins; D011663:Pulmonary Surfactants; D017830:Octoxynol; D004492:Edetic Acid