title: Automated urinalysis. Evaluation of the Sysmex UF-50.
abstract: We assessed the Sysmex UF-50 for reproducibility of results and carryover rate by performing between- and within-run precision analyses on 315 urine samples, evaluated the feasibility of using the UF-50 to measure urinary cellular and noncellular components by comparing results from the UF-50 with results of manual urinalysis using the Kova system, and performed side-by-side comparison of the within-run reproducibility from the UF-50, the UF-100, and the Kova system. Results from the UF-50 and UF-100 were highly reproducible, and the carryover rate was 0.5% or less for the urinary components. In between-run precision assays, the coefficients of variation for UF-50 results for all cellular components were less than 10%. The agreement (gamma statistics) between values from the UF-50 and the Kova system was excellent for RBC, WBC, and bacterial counts. The cell counts from the UF-50 for RBCs, WBCs, epithelial cells, and bacteria were 52%, 63%, 54%, and 110%, respectively, of those measured by manual urinalysis. The UF-50 performed quantitative analysis in 72 seconds, compared with 330 seconds for manual methods. The UF-50 is suitable for the first screening to detect hematuria, pyuria, and bacteriuria.
authors: Dept of Urology, Kobe University School of Medicine, 7-5-1, Kusunoki-cho, Chuoku, Kobe, Japan 650-0017.
authors: H
authors: H
authors: Okada
authors: Y
authors: Y
authors: Sakai
authors: G
authors: G
authors: Kawabata
authors: M
authors: M
authors: Fujisawa
authors: S
authors: S
authors: Arakawa
authors: Y
authors: Y
authors: Hamaguchi
authors: S
authors: S
authors: Kamidono
mesh_terms: D006801:Humans; D011786:Quality Control; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D016482:Urinalysis / Q000295:instrumentation* / Q000592:standards