title: Association of bovine papillomavirus type 1 with microtubules.
abstract: Transport of BPV-1 virus from the cell membrane to the nucleus was studied in vitro in CV-1 cells. At reduced temperature (4 degrees C), BPV-1 binding to CV-1 cells was unaffected but there was no transport of virions across the cytosol. Electron microscopy showed BPV-1 virions in association with microtubules in the cytoplasm, a finding confirmed by co-immunoprecipitation of L1 protein and tubulin. Internalization of virus was unimpaired in cells treated with the microtubule-depolymerizing drug nocodazole but virions were retained in cytoplasmic vesicles and not transported to the nucleus. We conclude that a microtubule transport mechanism in CV-1 cells moves intact BPV-1 virions from the cell surface to the nuclear membrane.
authors: Centre for Immunology and Cancer Research, University of Queensland, Woolloongabba, Queensland, 4102, Australia.
authors: W J
authors: WJ
authors: Liu
authors: Y M
authors: YM
authors: Qi
authors: K N
authors: KN
authors: Zhao
authors: Y H
authors: YH
authors: Liu
authors: X S
authors: XS
authors: Liu
authors: I H
authors: IH
authors: Frazer
mesh_terms: D021581:Active Transport, Cell Nucleus / Q000187:drug effects; D000818:Animals; D015153:Blotting, Western; D052688:Bovine papillomavirus 1 / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology / Q000648:ultrastructure; D002213:Capsid / Q000378:metabolism; D002417:Cattle; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism / Q000821:virology; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism / Q000821:virology; D022162:Cytoplasmic Vesicles / Q000187:drug effects / Q000378:metabolism / Q000821:virology; D019084:Fluorescent Antibody Technique, Indirect; D008854:Microscopy, Electron; D008870:Microtubules / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D015739:Nocodazole / Q000494:pharmacology; D017239:Paclitaxel / Q000494:pharmacology; D011485:Protein Binding; D013696:Temperature; D014404:Tubulin / Q000378:metabolism; D014771:Virion / Q000187:drug effects / Q000378:metabolism / Q000502:physiology / Q000648:ultrastructure; D014860:Warts / Q000821:virology
chemical_list: D014404:Tubulin; D017239:Paclitaxel; D015739:Nocodazole