Expression of neutrophil collagenase (MMP-8) in Jurkat T leukemia cells and its role in invasion.
BACKGROUND
Previous studies have shown that MMP-8, the neutrophil collagenase, was expressed in neutrophils, chondrocytes and rheumatoid synovial fibroblasts.


METHODS
We used semi-quantitative RT-PCR analysis, Western blotting, and immunofluorescence assays to determine the expression of MMP-8 in Jurkat T cells.


RESULTS
We have determined the expression of MMP-8 from Jurkat cells and the down-regulation of its expression by genistein, a principal soy isoflavone. Genistein inhibited the invasion of Jurkat cells through a model basement membrane by about 75%, similar to the inhibition by BB-94, a synthetic MMP inhibitor. Genistein also down-regulated the expression of MMP-13, but slightly up-regulated the expression of TIMP-1 and TIMP-2.


CONCLUSIONS
Our findings documented for the first time the expression of the neutrophil collagenase by a T-cell line. We also determined the inhibition of Jurkat cell invasion by genistein, which was in part mediated through the regulation of the expression of MMPs and TIMPs.
Kim
M H
MH
Department of Molecular Biology and Immunology and Institute for Cancer Research, University of North Texas Health Science Center at Fort Worth, 3500 Camp Bowie Blvd., Fort Worth, TX 76107-2699, USA.
Albertsson
P
P
Xue
Y
Y
Nannmark
U
U
Kitson
R P
RP
Goldfarb
R H
RH
D000970:Antineoplastic Agents / Q000494:pharmacology; D017364:Collagenases / Q000096:biosynthesis / Q000235:genetics; D015536:Down-Regulation; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology; D015972:Gene Expression Regulation, Neoplastic; D019833:Genistein / Q000494:pharmacology; D006801:Humans; D019169:Jurkat Cells; D015458:Leukemia, T-Cell / Q000201:enzymology* / Q000235:genetics* / Q000473:pathology; D053509:Matrix Metalloproteinase 13; D020784:Matrix Metalloproteinase 8 / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D009361:Neoplasm Invasiveness; D019714:Tissue Inhibitor of Metalloproteinases / Q000096:biosynthesis / Q000235:genetics
D000970:Antineoplastic Agents; D016326:Extracellular Matrix Proteins; D019714:Tissue Inhibitor of Metalloproteinases; D019833:Genistein; D017364:Collagenases; C507079:MMP13 protein, human; D053509:Matrix Metalloproteinase 13; D020784:Matrix Metalloproteinase 8