Genotype-dependent differences in S12-RNase expression lead to sporadic self-compatibility.
Sporadic self-compatibility, the occasional fruit formation after otherwise incompatible pollinations, has been observed in some S12-containing genotypes of Solanum chacoense but not in others. We have sequenced this S12 allele and analyzed its expression in four different genotypes. The S12-RNase levels were generally less abundant than those of other S-RNases present in the same plants. In addition, two-fold and five-fold differences in the amount of S12-RNase and S12 RNA, respectively, were observed among the genotypes analyzed. A comparison with the genetic data showed that genotypes with the highest levels were fully and permanently self-incompatible, whereas those with the lowest levels were those in which sporadic self-compatibility had been observed. The mature protein contains four potential glycosylation sites and genotype-specific differences in the pattern of glycosylation are also observed. Our results suggest the presence of modifier genes which affect, in a genotype-dependent manner, the level of expression and the post-translational modification of the S12-RNase.
Qi
X
X
Département de Sciences Biologiques, Université de Montréal Québec, Canada.
Luu
D T
DT
Yang
Q
Q
Maës
O
O
Matton
D P
DP
Morse
D
D
Cappadocia
M
M
D000483:Alleles; D000595:Amino Acid Sequence; D001483:Base Sequence; D003433:Crosses, Genetic; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D005838:Genotype; D006031:Glycosylation; D008969:Molecular Sequence Data; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D012098:Reproduction / Q000235:genetics; D012260:Ribonucleases / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA; D019657:Solanaceae / Q000235:genetics*
D018076:DNA, Complementary; D018744:DNA, Plant; D018749:RNA, Plant; D012260:Ribonucleases; C424719:S12 RNase; C022542:ribonuclease S