Activation of the Sarcophaga lectin gene promoter by (A + T)-stretch binding protein.
Previously, we purified and isolated a cDNA for (A + T)-stretch binding protein (ATBP) that binds to (A + T)-stretches in the 5' upstream region of the Sarcophaga lectin gene [Nakanishi-Matsui, M., Kubo, T. & Natori, S. (1995) Eur. J. Biochem. 230, 396-400]. Here, we used a luciferase reporter to examine the effect of ATBP on transcription of the Sarcophaga lectin gene. Deletion experiments revealed that ATBP activates the Sarcophaga lectin gene in a 5' upstream sequence-dependent manner, and that at least the N-terminal 25 residues, the three Zn-finger domains, an acidic domain and the third hydrophobic domain of ATBP are indispensable for its function. Furthermore, a synergistic effect was detected between ATBP and Dif, suggesting that ATBP is involved in the activation of insect immunity genes.
Aozasa
N
N
Graduate School of Pharmaceutical Sciences, University of Tokyo, Bunkyo-ku, Tokyo 113-0033; Natori Special Laboratory, The Institute of Physical and Chemical Research, Hirosawa 2-1, Wako-shi, Saitama 351-0198, Japan.
Shiraishi
H
H
Nakanishi-Matsui
M
M
Kobayashi
A
A
Sekimizu
K
K
Kubo
T
T
Natori
S
S
D000818:Animals; D002478:Cells, Cultured; D018076:DNA, Complementary / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D004330:Drosophila; D017353:Gene Deletion; D017930:Genes, Reporter; D019476:Insect Proteins; D037102:Lectins / Q000235:genetics*; D008156:Luciferases / Q000378:metabolism; D009687:Nuclear Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D017434:Protein Structure, Tertiary; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic; D014162:Transfection; D016335:Zinc Fingers
C094603:ATBP protein, Sarcophaga peregrina; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D019476:Insect Proteins; D037102:Lectins; D009687:Nuclear Proteins; D014157:Transcription Factors; D008156:Luciferases