title: Varying competence for protein import into chloroplasts during the cell cycle in Chlamydomonas.
abstract: By studying the import of radioactively labelled small subunit of ribulose-1,5-bisphosphate carboxylase (pSS) into chloroplasts of the green alga C. reinhardtii cw-15 protein delivery to chloroplasts was found to vary during the cell cycle. Chloroplasts were isolated from highly synchronous cultures at different time points during the cell cycle. When pSS was imported into 'young' chloroplasts isolated early in the light period about three times less pSS was processed to small subunit SS than in 'mature' chloroplasts from the middle of the light period. In 'young' chloroplasts also, less pSS was bound to the envelope surface. During the second half of the light period the import competence of isolated chloroplasts decreased again when based on chlorophyll content or cell volume, but did not change significantly when related to chloroplast number. Measurements of pSS binding to the surface of chloroplasts of different age indicated that the adaptation of protein import competence during the cell cycle is due to a variation of the number of binding sites per chloroplast surface area, rather than to modulation of the binding constant.
authors: Departement für Chemie und Biochemie, Universität Bern, Switzerland.
authors: Q
authors: Q
authors: Su
authors: C
authors: C
authors: Schild
authors: P
authors: P
authors: Schumann
authors: A
authors: A
authors: Boschetti
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D001665:Binding Sites; D002453:Cell Cycle; D002455:Cell Division; D002696:Chlamydomonas / Q000378:metabolism*; D002736:Chloroplasts / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D007700:Kinetics; D010100:Oxygen / Q000378:metabolism; D010788:Photosynthesis / Q000502:physiology; D010957:Plasmids / Q000378:metabolism; D011485:Protein Binding; D021381:Protein Transport; D012273:Ribulose-Bisphosphate Carboxylase / Q000378:metabolism*; D013997:Time Factors
chemical_list: D000255:Adenosine Triphosphate; D012273:Ribulose-Bisphosphate Carboxylase; D010100:Oxygen