Microdissection and reverse painting in a melanoma cell line: a detailed description of structurally abnormal chromosomes.
Seven structurally abnormal chromosomes from a newly established melanoma cell line were microdissected. The DNA from these chromosomes was DOP amplified and labeled with Biotin or Digoxigenin for FISH analysis. The complex nature of these markers is described.
Friedrich
U
U
Institute of Human Genetics, The Bartholin Building, University of Aarhus, DK-8000, Aarhus C, Denmark. zitta@humgen.au.dk
Houman
M
M
Hansen
B H
BH
Kaltoft
K
K
D002869:Chromosome Aberrations; D025063:Chromosome Disorders; D020223:Chromosome Painting; D006801:Humans; D007621:Karyotyping; D008545:Melanoma / Q000235:genetics* / Q000473:pathology; D014407:Tumor Cells, Cultured