title: Repair of cartilage defect in the rabbit with cultured mesenchymal stem cells from bone marrow.
abstract: In 16 mature New Zealand white rabbits mesenchymal stem cells were aspirated from the bone marrow, cultured in monolayer and implanted on to a full-thickness osteochondral defect artificially made on the patellar groove of the same rabbit. A further 13 rabbits served as a control group. The rabbits were killed after 14 weeks. Healing of the defect was investigated histologically using haematoxylin and eosin and Safranin-O staining and with immunohistochemical staining for type-II collagen. We also used a reverse transcription-polymerase chain reaction (RT-PCR) to detect mRNA of type-I and type-II collagen. The semiquantitative histological scores were significantly higher in the experimental group than in the control group (p < 0.05). In the experimental group immunohistochemical staining on newly formed cartilage was more intense for type-II collagen in the matrix and RT-PCR from regenerated cartilage detected mRNA for type-II collagen in mature chondrocytes. These findings suggest that repair of cartilage defects can be enhanced by the implantation of cultured mesenchymal stem cells.
authors: Hallym University, Chunchon, Korea.
authors: G I
authors: GI
authors: Im
authors: D Y
authors: DY
authors: Kim
authors: J H
authors: JH
authors: Shin
authors: C W
authors: CW
authors: Hyun
authors: W H
authors: WH
authors: Cho
mesh_terms: D000818:Animals; D016026:Bone Marrow Transplantation; D002356:Cartilage / Q000293:injuries*; D003094:Collagen / Q000032:analysis; D018380:Hematopoietic Stem Cell Transplantation; D007150:Immunohistochemistry; D008648:Mesoderm / Q000166:cytology; D016133:Polymerase Chain Reaction; D011817:Rabbits; D014182:Transplantation, Autologous
chemical_list: D003094:Collagen