Micronucleus formation in human lymphocytes and in the metabolically competent human hepatoma cell line Hep-G2: results with 15 naturally occurring substances.
To examine the concordance of two metabolizing systems for use in genotoxocity testing with the micronucleus test, 15 naturally occurring substances (arecoline, the plant extract aristolochic acid, beta-asarone, benzyl acetate, coumarin, emodine, isatidine dihydrate, monocrotaline, psoralen, reserpine, retrorsine, safrole, sanguinarine chloride, tannin and thiourea) were tested for their genotoxicity in the cytokinesis-block micronucleus test in vitro with human lymphocytes and in the presence and the absence of an exogenous metabolizing system from rat liver S9-mix and the metabolically competent human hepatoma cell line Hep-G2. Arecoline, the plant extract aristolochic acid, psoralen and tannin caused a significant increase in the number of micronuclei in human lymphocytes in the presence and the absence of an exogenous metabolising system from rat liver S9-mix and the metabolically competent human hepatoma cell line Hep-G2. A significant increase in the number of micronuclei with beta-asarone, coumarin, monocrotaline and retrorsine could be detected in the presence of S9-mix and the cell line Hep-G2. Benzyl acetate, emodine, isatidine dihydrate, reserpine, safrole, sanguinarine chloride and thiourea did not reveal any micronucleus inducing activity in either human lymphocytes or in Hep-G2. In addition to the other Hep-G2 results in the literature, this human hepatoma cell line could have a useful potential in the in vitro micronucleus test.
Kevekordes
S
S
Medical Institute of General Hygiene and Environmental Health, University of Göttingen, Windausweg 2, 37073 Göttingen, Germany. skeveko@gmx.net
Spielberger
J
J
Burghaus
C M
CM
Birkenkamp
P
P
Zietz
B
B
Paufler
P
P
Diez
M
M
Bolten
C
C
Dunkelberg
H
H
D000328:Adult; D000818:Animals; D001685:Biological Factors / Q000633:toxicity; D001688:Biological Products / Q000633:toxicity; D006528:Carcinoma, Hepatocellular; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008110:Liver Extracts / Q000378:metabolism; D008214:Lymphocytes / Q000187:drug effects / Q000648:ultrastructure; D015162:Micronucleus Tests / Q000379:methods*; D009153:Mutagens / Q000633:toxicity*; D051381:Rats; D014407:Tumor Cells, Cultured
D001685:Biological Factors; D001688:Biological Products; D008110:Liver Extracts; D009153:Mutagens