title: Physical properties of erythrocyte ghosts that determine susceptibility to secretory phospholipase A2.
abstract: Artificial membranes may be resistant or susceptible to catalytic attack by secretory phospholipase A(2) (sPLA(2)) depending on the physical properties of the membrane. Living cells are normally resistant but become susceptible during trauma, apoptosis, and/or a significant elevation of intracellular calcium. Intact erythrocytes and ghosts were studied to determine whether the principles learned from artificial systems apply to biological membranes. Membrane properties such as phospholipid and/or protein composition, morphology, and microscopic characteristics (e.g. fluidity) were manipulated by preparing ghosts under different experimental conditions such as in the presence or absence of divalent cations with or without ATP. The properties of each membrane preparation were assessed by biochemical and physical means (fluorescence spectroscopy and electron and two-photon microscopy using the membrane probes bis-pyrene and laurdan) and compared with sPLA(2) activity. The properties that appeared most relevant were the degree of phosphatidylserine exposure on the outer face of the membrane and changes to the membrane physical state detected by bis-pyrene and laurdan. Specifically, vulnerability to hydrolysis by sPLA(2) was associated with an increase in bilayer order apparently reflective of expansion of membrane regions of diminished fluidity. These results argue that the general principles identified from studies with artificial membranes apply to biological systems.
authors: Department of Zoology, Brigham Young University, Provo, Utah 84602, USA.
authors: F M
authors: FM
authors: Harris
authors: S K
authors: SK
authors: Smith
authors: J D
authors: JD
authors: Bell
mesh_terms: D004910:Erythrocyte Membrane / Q000737:chemistry* / Q000187:drug effects* / Q000648:ultrastructure; D008560:Membrane Fluidity / Q000187:drug effects; D008855:Microscopy, Electron, Scanning; D010741:Phospholipases A / Q000494:pharmacology*; D054467:Phospholipases A2
chemical_list: D010741:Phospholipases A; D054467:Phospholipases A2