PspC, a pneumococcal surface protein, binds human factor H.
PspC was found to bind human complement factor H (FH) by Western blot analysis of D39 (pspC(+)) and an isogenic mutant TRE108 (pspC). We confirmed that PspA does not bind FH, while purified PspC binds FH very strongly. The binding of FH to exponentially growing pneumococci varied among different isolates when analyzed by fluorescence activated cell sorting analysis.
Dave
S
S
Department of Microbiology, The University of Mississippi Medical Center, Jackson, Mississippi 39216, USA.
Brooks-Walter
A
A
Pangburn
M K
MK
McDaniel
L S
LS
D001426:Bacterial Proteins / Q000378:metabolism*; D015153:Blotting, Western; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D017242:Complement Factor H / Q000378:metabolism*; D006801:Humans; D015800:Protozoan Proteins
D001426:Bacterial Proteins; C493495:CFH protein, human; D002135:Calcium-Binding Proteins; D015800:Protozoan Proteins; C110344:SpsA protein, Streptococcus pneumoniae; C093239:calcium-binding protein 1, Dictyostelium discoideum; C051592:pneumococcal surface protein A; D017242:Complement Factor H