Norepinephrine-induced migration of SW 480 colon carcinoma cells is inhibited by beta-blockers.
Beta-adrenoceptors are highly expressed on SW 480 colon carcinoma cells as was assessed by flow cytometry. We investigated the influence of norepinephrine on the migration of these cells using time-lapse videomicroscopy. Norepinephrine-treatment increased the locomotor activity within the population from 25% spontaneously locomoting cells to 65% locomoting cells. The beta1/2-blocker propranolol but not the beta1-blocker atenolol inhibited this increase. The intracellular signaling solely of norepinephrine-induced locomotion involved protein tyrosine kinase activity, whereas both spontaneous and norepinephrine-induced migration were reduced by inhibiting phospholipase Cgamma and protein kinase Calpha activity. In summary, norepinephrine-induced locomotion of SW 480 cells is beta2-adrenoceptor mediated and distinct from spontaneous locomotion concerning the PTK involvement.
Masur
K
K
Institute for Immunology, Witten/Herdecke University, Witten, Germany. kaimasur@uni-wh.de
Niggemann
B
B
Zanker
K S
KS
Entschladen
F
F
D000316:Adrenergic alpha-Agonists / Q000494:pharmacology*; D000319:Adrenergic beta-Antagonists / Q000494:pharmacology*; D002465:Cell Movement / Q000187:drug effects*; D003110:Colonic Neoplasms / Q000378:metabolism / Q000473:pathology*; D004347:Drug Interactions; D005434:Flow Cytometry; D009638:Norepinephrine / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D011942:Receptors, Adrenergic, alpha / Q000096:biosynthesis / Q000145:classification; D011943:Receptors, Adrenergic, beta / Q000096:biosynthesis / Q000145:classification; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology
D000316:Adrenergic alpha-Agonists; D000319:Adrenergic beta-Antagonists; D011942:Receptors, Adrenergic, alpha; D011943:Receptors, Adrenergic, beta; D009638:Norepinephrine