Molecular cloning of a cDNA coding for feline liver xanthine dehydrogenase.
A cDNA coding for feline liver xanthine dehydrogenase (XDH, EC 1.1.204) was amplified by RT-PCR and cloned for determining the sequence. The clones contained an open reading frame of 4002 base pairs encoding 1333 amino acid residues. The calculated molecular weight and isoelectric point were approximately 146 kDa and 7.0. Comparison of the deduced amino acid sequences indicated remarkable high homology, i.e., the amino acid residues of feline XDH shared approximately 90%, 87%, 87% and 86% identity with those of human, bovine, rat and mouse, respectively. The anino acid sequences of two putative iron-sulfur centers, one NAD binding site and one molybdenum binding site were well conserved among mammalian animals.
Tsuchida
S
S
Division of Veterinary Surgery, Nippon Veterinary and Animal Science University, Musashino, Tokyo, Japan.
Yamada
R
R
Ikemoto
S
S
Tagawa
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002415:Cats / Q000378:metabolism*; D002417:Cattle; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D006801:Humans; D008099:Liver / Q000201:enzymology*; D051379:Mice; D008969:Molecular Sequence Data; D012313:RNA / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014968:Xanthine Dehydrogenase / Q000737:chemistry / Q000235:genetics*
D018076:DNA, Complementary; D012313:RNA; D014968:Xanthine Dehydrogenase