Involvement of the heparan sulphate-binding proteins of Helicobacter pylori in its adherence to HeLa S3 and Kato III cell lines.
To determine whether Helicobacter pylori heparan sulphate-binding proteins (HSBPs) are involved in the adherence of H. pylori to HeLa and Kato III cells, monolayers were pre-incubated with various preparations and concentrations of H. pylori HSBPs at 37 degrees C, washed and then challenged with bacteria. HSBPs did not prevent but enhanced H. pylori adherence. However, challenging cultured cells with H. pylori previously incubated with rabbit anti-HSBP IgG resulted in significant inhibition of bacterial adherence. These data demonstrate that the extracellular HSBP plays an important role in promoting H. pylori attachment to Kato III and HeLa S3 cells, that adhesion of H. pylori to Kato III and HeLa S3 cells is promoted by the presence of the 71.5-kDa extracellular HSBP and that rabbit polyclonal antibodies against this HSBP can inhibit adhesion of H. pylori to the cultured cell lines and detach cell-bound H. pylori.
Guzman-Murillo
Maria A
MA
Ruiz-Bustos
Eduardo
E
Ho
Bow
B
Center for Biological Research, Marine Pathology Unit, La Paz, BCS 23000, Mexico and *Department of Microbiology, National University of Singapore, Singapore 117597.
Ascencio
Felipe
F
Center for Biological Research, Marine Pathology Unit, La Paz, BCS 23000, Mexico and *Department of Microbiology, National University of Singapore, Singapore 117597.
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D001422:Bacterial Adhesion / Q000502:physiology*; D002352:Carrier Proteins / Q000276:immunology / Q000378:metabolism*; D006367:HeLa Cells; D016481:Helicobacter Infections / Q000382:microbiology; D016480:Helicobacter pylori / Q000472:pathogenicity / Q000502:physiology*; D006497:Heparan Sulfate / Q000378:metabolism*; D006801:Humans; D007422:Intestines / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D014407:Tumor Cells, Cultured
D000907:Antibodies, Bacterial; D002352:Carrier Proteins; D006497:Heparan Sulfate