[Mechanism of thiol-dependence of acute phase proteins and serology of monospecific antisera in vitro].
For the donors and for the patients with inflammatory processes is thiol-dependent the gear of immune responses in vitro an antigen--antibody on dynamics(changes) of change (+/- delta) of the contents SH- and S-S-group reaction mixtures. Thus, is conducted the analysis of interplay of proteins of an acute phase (CRP, orosomucoid and transferin) serums of a blood of the donors and patients with serology by related diagnostic (complementary) monospecific serums (MSS) against CRP (Anti-CRP), against Oroso (Anti-Oroso) i against Transf (Anti-Transf). Is established, that as against the donors, for the patients with inflammatory processes these reacting are accompanied by a phenomenon of a liberation of energy of Ag(+)-sensing non proteins SH-groups and they occur in supernatants of deprotheinized of reaction mixtures. At the same time, both for the donors, and for the patients, these reacting are accompanied modification by changes kept in repair (+/- delta) proteins SH- and S-S-rpy[symbol: see text], in integral reaction mixtures (in which one protein did not deposit). Such data testify, that the inflammatory process, apparently, can be accompanied by such rearrangement of a structurally functional condition of proteins of an acute phase, that under operating MSS in reaction mixtures descends labelised blended disulfide of communications between them and low molecular weight thiols. As a result of it there is a liberation of energy of Ag(+)-sensing non proteins SH-groups. This parameter can be used for an estimation of functional activity of proteins of an acute phase.
Kostiushov
V V
VV
411 Central (Military) Clinical Hospital, Odesa.
Kostiushova
N V
NV
Pavlovich
S I
SI
Sakhno
Iu P
IuP
Tymchyshyn
O L
OL
D000209:Acute-Phase Proteins / Q000276:immunology / Q000378:metabolism*; D000210:Acute-Phase Reaction / Q000097:blood / Q000276:immunology* / Q000378:metabolism; D000937:Antigen-Antibody Reactions; D001782:Blood Donors; D001798:Blood Proteins / Q000276:immunology / Q000378:metabolism*; D002097:C-Reactive Protein / Q000276:immunology / Q000378:metabolism; D006801:Humans; D007249:Inflammation / Q000097:blood / Q000276:immunology*; D009961:Orosomucoid / Q000276:immunology / Q000378:metabolism; D013438:Sulfhydryl Compounds / Q000097:blood / Q000378:metabolism*; D014168:Transferrin / Q000276:immunology / Q000378:metabolism
D000209:Acute-Phase Proteins; D001798:Blood Proteins; D009961:Orosomucoid; D013438:Sulfhydryl Compounds; D014168:Transferrin; D002097:C-Reactive Protein