Chromosomal positioning of human T-lymphotropic type 1 proviruses by fluorescence in situ hybridisation.
Fluorescence in situ hybridisation (FISH) was employed to identify the chromosomal integration site of the human T-cell lymphotropic virus, type 1 (HTLV-1) present in T-cell clones derived from HTLV-1-infected individuals and a virally transformed cell line, C8166-45. Proviral sequences were detected in C8166-45 but not uninfected Jurkat cells. Integration sites were reliably detected in T-cell clones determined previously to be infected with HTLV-1. The results indicated that the transformed cell line and some of the T-cell clones possessed more than one proviral integration site. This hybridisation system is useful for determining the number of integration events and for localising proviruses to specific chromosomal regions.
Richardson
J H
JH
University of Cambridge, Department of Medicine, Level 5, Addenbrooke's Hospital, Hills Road, Cambridge CB2 2QQ, UK.
Rose
N J
NJ
Mann
S
S
Ferguson-Smith
M
M
Lever
A M
AM
D002461:Cell Line, Transformed; D002875:Chromosomes / Q000821:virology*; D002999:Clone Cells; D015368:Human T-lymphotropic virus 1 / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D019169:Jurkat Cells; D011533:Proviruses / Q000302:isolation & purification*; D013601:T-Lymphocytes / Q000821:virology; D016662:Virus Integration