Mapping tertiary interactions in protein folding reactions: a novel mass spectrometry- and chemical synthesis-based approach.
A novel mass spectrometry- and chemical synthesis-based approach for studying protein folding reactions is described, and its initial application to study the folding/unfolding reaction of a homo-hexameric enzyme 4-oxalocrotonate (4OT) is reported. This new approach involves the application of total chemical synthesis to prepare protein analogues that contain a photoreactive amino acid site-specifically incorporated into their primary amino acid sequence. To this end, a photoreactive amino acid-containing analogue of 4OT in which Pro-1 was replaced with p-benzoyl-l-phenylalanine (Bpa) was prepared. This analogue can be used to map structurally specific protein-protein interactions in 4OT's native folded state. These photocrosslinking studies and peptide mapping results with (PlBpa)4OT indicate that this construct is potentially useful for probing the structural properties of equilibrium and kinetic intermediates in 4OT's folding reaction.
Moss
J A
JA
Department of Chemistry, Duke University, Durham, North Carolina 27708, USA.
Silinski
P
P
Fitzgerald
M C
MC
D002850:Chromatography, Gel; D002942:Circular Dichroism; D019281:Dimerization; D007535:Isomerases / Q000737:chemistry*; D010446:Peptide Fragments / Q000737:chemistry; D010449:Peptide Mapping; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry*; D017510:Protein Folding; D017434:Protein Structure, Tertiary; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D013053:Spectrophotometry
D010446:Peptide Fragments; D010455:Peptides; C021918:4-oxalocrotonate tautomerase; D007535:Isomerases