Identifying sex chromosome abnormalities in forensic DNA testing using amelogenin and sex chromosome short tandem repeats.
Forensic DNA laboratories worldwide have begun using multiplexed STR systems to decrease analysis time and increase sample throughput. The loci used in these systems are basically "nonsense" regions of human DNA. However, due to the chromosome on which some of these loci are located, various genetic abnormalities can sometimes be detected. This paper will show one such abnormality--Klinefelter's Syndrome--and the process used to show the possibility of this defect in two undiagnosed males using peak height ratios at the Amelogenin locus, and X-Y STRs.
Young
D R
DR
Texas Department of Public Safety Crime Laboratory, Lubbock 79408, USA. david.young@txdps.state.tx.us
Tun
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Z
Honda
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K
Matoba
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D053523:Amelogenin; D002869:Chromosome Aberrations / Q000235:genetics*; D025063:Chromosome Disorders; D004247:DNA / Q000235:genetics; D016172:DNA Fingerprinting; D003746:Dental Enamel Proteins; D005260:Female; D005554:Forensic Medicine / Q000379:methods; D006801:Humans; D007713:Klinefelter Syndrome / Q000235:genetics; D008297:Male; D016133:Polymerase Chain Reaction; D012730:Sex Chromosomes / Q000235:genetics*; D020080:Tandem Repeat Sequences / Q000235:genetics*
D053523:Amelogenin; D003746:Dental Enamel Proteins; D004247:DNA