[The relationship between the mutation of methylenetetrahydrofolate reductase gene 677C-->T and the diabetic microangiopathy].
OBJECTIVE
To investigate the relationship between the mutation of methylenetetra- hydrofolate reductase gene 677C-->T and the diabetic microangiopathy(DMA) in diabetes mellitus(DM).


METHODS
A total of 168 subjects were divided into control group, DM group and DMA group(including diabetic nephropathy and diabetic retinopathy). PCR-restrictive fragment length polymorphism(RFLP) analysis was conducted to examine mutation, and then the frequency of mutation was statistically computed.


RESULTS
Markedly elevated mutation was observed in patients with diabetic nephropathy and diabetic retinopathy as compared with non-microangiopathy diabetes mellitus and normal people (20.8% vs 8.3% and 7.3%, P<0.01). The odds ratio of the TT genotype for DMA was 3.36, P<0.001.


CONCLUSIONS
It was found that the mutation of methylenetetrahydrofolate reductase gene 677C-->T was an impressibility factor of diabetic microangiopathy.
Hu
S
S
Department of Endocrinology, People's Hospital, Wuhan University, Wuhan, Hubei 430060 P. R. China. husheng 71@263.net
Gan
P
P
Li
J
J
Bi
H
H
D000328:Adult; D000368:Aged; D003925:Diabetic Angiopathies / Q000235:genetics*; D005260:Female; D006801:Humans; D008297:Male; D042965:Methylenetetrahydrofolate Reductase (NADPH2); D008875:Middle Aged; D009154:Mutation; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000235:genetics*; D012044:Regression Analysis
D000587:Oxidoreductases Acting on CH-NH Group Donors; D042965:Methylenetetrahydrofolate Reductase (NADPH2)