title: Epstein--Barr virus gene polymorphisms in Chinese Hodgkin's disease cases and healthy donors: identification of three distinct virus variants.
abstract: Epstein--Barr virus (EBV) is associated with several malignancies. Specific EBV gene variants, e.g. the BamHI f configuration, a C-terminal region 30 bp deletion in the latent membrane protein-1 (LMP1) gene (del-LMP) and the loss of an XhoI site in LMP1 (XhoI-loss), are found in Chinese cases of nasopharyngeal carcinoma (NPC), suggesting that EBV sequence variation may be involved in oncogenesis. In order to understand better the epidemiology of these EBV variants, they were studied in virus isolates from EBV-positive Chinese cases of Hodgkin's disease (HD; n=71) and donor throat washings from healthy CHINESE: Sequencing was performed of 15 representative EBV isolates, including the first analysis of the LMP1 promoter in Asian wild-type EBV isolates. The following observations were made. (i) Three EBV LMP1 variants were identified, designated Chinese groups (CG) 1--3. In both EBV-associated HD and in healthy Chinese, CG1-like viruses showing del-LMP1 and XhoI-loss were predominant. (ii) CG1viruses were distinct from European and African variants, suggesting that this profile is useful for epidemiological studies. (iii) Specific patterns of mutations were present in the LMP1 promoter in both CG1 and CG2. (iv) The BamHI f variant was not found in Chinese HD, in contrast to Chinese NPC and European HD. This study confirms that EBV isolates in Chinese HD and other tumours differ from those reported in Western cases. However, this reflects the predominant virus strain present in the healthy Chinese population, suggesting that these are geographically restricted polymorphisms rather than tumour-specific strains.
authors: Departments of Pathology of Beijing Hospital3, Beijing Children's Hospital4, Beijing 301 Hospital5, Beijing Railway General Hospital6, Wunancabumong District Hospital7 and Beijing Air Army General Hospital8, People's Republic of China.|Research Unit of Molecular Medicine, Aarhus University Hospital and Faculty of Health Sciences, Aarhus, Denmark2.|Institute of Pathology, Aarhus Kommunehospital, Noerrebrogade 44, DK-8000 Aarhus C, Denmark1.
authors: X-G
authors: XG
authors: Zhou
authors: Institute of Pathology, Aarhus Kommunehospital, Noerrebrogade 44, DK-8000 Aarhus C, Denmark1.
authors: K
authors: K
authors: Sandvej
authors: Departments of Pathology of Beijing Hospital3, Beijing Children's Hospital4, Beijing 301 Hospital5, Beijing Railway General Hospital6, Wunancabumong District Hospital7 and Beijing Air Army General Hospital8, People's Republic of China.
authors: P-J
authors: PJ
authors: Li
authors: Departments of Pathology of Beijing Hospital3, Beijing Children's Hospital4, Beijing 301 Hospital5, Beijing Railway General Hospital6, Wunancabumong District Hospital7 and Beijing Air Army General Hospital8, People's Republic of China.
authors: X-L
authors: XL
authors: Ji
authors: Departments of Pathology of Beijing Hospital3, Beijing Children's Hospital4, Beijing 301 Hospital5, Beijing Railway General Hospital6, Wunancabumong District Hospital7 and Beijing Air Army General Hospital8, People's Republic of China.
authors: Q-H
authors: QH
authors: Yan
authors: Departments of Pathology of Beijing Hospital3, Beijing Children's Hospital4, Beijing 301 Hospital5, Beijing Railway General Hospital6, Wunancabumong District Hospital7 and Beijing Air Army General Hospital8, People's Republic of China.
authors: X-P
authors: XP
authors: Zhang
authors: Departments of Pathology of Beijing Hospital3, Beijing Children's Hospital4, Beijing 301 Hospital5, Beijing Railway General Hospital6, Wunancabumong District Hospital7 and Beijing Air Army General Hospital8, People's Republic of China.
authors: J-P
authors: JP
authors: Da
authors: Institute of Pathology, Aarhus Kommunehospital, Noerrebrogade 44, DK-8000 Aarhus C, Denmark1.
authors: S J
authors: SJ
authors: Hamilton-Dutoit
mesh_terms: D044466:Asian People; D001483:Base Sequence; D004279:DNA, Viral; D015245:Deoxyribonuclease BamHI; D015252:Deoxyribonucleases, Type II Site-Specific; D005814:Genes, Viral; D014644:Genetic Variation; D006304:Health Status; D004854:Herpesvirus 4, Human / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D006689:Hodgkin Disease / Q000473:pathology / Q000821:virology*; D006801:Humans; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D011401:Promoter Regions, Genetic; D017384:Sequence Deletion; D014763:Viral Matrix Proteins / Q000235:genetics
chemical_list: D004279:DNA, Viral; C037662:EBV-associated membrane antigen, Epstein-Barr virus; D014763:Viral Matrix Proteins; D015245:Deoxyribonuclease BamHI; C034510:CTCGAG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific