Simultaneous evaluation of dual gene transfer to adherent cells by gamma-ray imaging.
A gamma camera imaging method was developed to detect dual gene transfer to adherent cells growing as monolayers in cell culture plates. Human cancer cells were infected with replication-incompetent adenoviral vectors encoding the human type 2 somatostatin receptor (Ad-hSSTr2) and/or herpes virus thymidine kinase (Ad-TK). The hSSTr2 and TK reporter proteins were detected by imaging internally bound (99m)Tc-P2045 peptide (Diatide, Inc.) and radioiodinated 2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl-5-iodouracil (FIAU), respectively. Following gene transfer, expression of hSSTr2 and TK were accurately imaged in vitro.
Zinn
K R
KR
Department of Radiology, University of Alabama at Birmingham, Birmingham, Alabama, USA. kurtzinn@uab.edu
Chaudhuri
T R
TR
Buchsbaum
D J
DJ
Mountz
J M
JM
Rogers
B E
BE
D000256:Adenoviridae; D001086:Arabinofuranosyluracil / Q000031:analogs & derivatives* / Q000493:pharmacokinetics; D002289:Carcinoma, Non-Small-Cell Lung; D002448:Cell Adhesion; D002460:Cell Line; D015902:Gamma Cameras; D005720:Gamma Rays; D005822:Genetic Vectors; D006564:Herpesviridae / Q000235:genetics; D006801:Humans; D007457:Iodine Radioisotopes / Q000493:pharmacokinetics; D007668:Kidney; D008175:Lung Neoplasms; D019275:Radiopharmaceuticals; D017481:Receptors, Somatostatin / Q000235:genetics*; D013667:Technetium / Q000493:pharmacokinetics; D013937:Thymidine Kinase / Q000235:genetics*; D014162:Transfection / Q000379:methods*; D014407:Tumor Cells, Cultured
D007457:Iodine Radioisotopes; D019275:Radiopharmaceuticals; D017481:Receptors, Somatostatin; D001086:Arabinofuranosyluracil; C043457:fialuridine; D013667:Technetium; C093733:somatostatin receptor 2; D013937:Thymidine Kinase