[Clinical usefulness of antigen-captured ELISA method using mouse anti-Trichosporon asahii monoclonal antibody D-8 for diagnosis of summer-type hypersensitivity pneumonitis].
In the present study, we assessed the clinical usefulness of the antigen-captured ELISA method using mouse anti-Trichosporon asahii monoclonal antibody D-8 in 193 patients with summer-type hypersensitivity pneumonitis (SHP) and in 127 healthy volunteers. When the cut-off value was determined as 0.15, the diagnosis of SHP was accurately made by this method in 168 of 193 SHP patients. The sensitivity and specificity of the diagnosis of SHP determined by this method were 87.0% and 96.1%, respectively. We concluded that this ELISA method is a useful technique for the diagnosis of SHP when the possibility of SHP is recognized during the course of a case.
Miyake
S
S
Department of Pulmonary Medicine, Tokyo Medical and Dental University, Japan.
Hamaoka
A
A
Yoshizawa
Y
Y
D000542:Alveolitis, Extrinsic Allergic / Q000175:diagnosis*; D000818:Animals; D000908:Antibodies, Fungal / Q000032:analysis; D000911:Antibodies, Monoclonal; D015415:Biomarkers / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D051379:Mice; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D014250:Trichosporon / Q000276:immunology*
D000908:Antibodies, Fungal; D000911:Antibodies, Monoclonal; D015415:Biomarkers; D007074:Immunoglobulin G; D011933:Reagent Kits, Diagnostic