Detection and characterization of quorum sensing signal molecules in Acinetobacter strains.
Quorum sensing is a widespread regulatory mechanism among Gram-negative bacteria. In this study, Acinetobacter strains were assayed for the presence of quorum sensing signal molecules capable of activating N-acylhomoserine lactone biosensors. By using an Agrobacterium tumefaciens reporter strain it was shown that all the cultures produced two to four detectable signal molecules with different chromatographic patterns. In A. calcoaceticus BD413 supernatants four compounds were detected in a time-dependent manner, and maximal activity was reached at stationary phase. The number of signal molecules was dependent on medium composition; typically, cultures in minimal medium displayed one or two more signals, as compared to complex medium. None of the Acinetobacter supematants showed autoinduction activity with an Chromobacterium violaceum reporter strain, neither in direct or competition assays.
González
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RH
Department of Industrial Microbiology and Biotechnology, Faculty of Pharmacy and Biochemistry, University of Buenos Aires, Argentina.
Nusblat
A
A
Nudel
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BC
D000150:Acinetobacter / Q000254:growth & development / Q000378:metabolism*; D015374:Biosensing Techniques; D002855:Chromatography, Thin Layer; D017077:Culture Media, Conditioned; D006714:Homoserine / Q000031:analogs & derivatives / Q000032:analysis / Q000378:metabolism; D006801:Humans; D007783:Lactones / Q000032:analysis / Q000378:metabolism; D015398:Signal Transduction; D013997:Time Factors
D017077:Culture Media, Conditioned; D007783:Lactones; D006714:Homoserine