title: Molecular characterization of fibroblasts isolated from human peritoneum and adhesions.
abstract: OBJECTIVE
  To determine the response of adhesion and peritoneal fibroblasts to hypoxia.
  
  
  METHODS
  Prospective experimental study.
  
  
  METHODS
  University medical center.
  
  
  METHODS
  Primary cultures of fibroblasts established from the peritoneal and adhesion tissues of the same patients (n = 2) to minimize genetic variations.
  
  
  METHODS
  Hypoxia treatment of the primary cultured fibroblast.
  
  
  METHODS
  Analyze the expression of extracellular matrix (ECM) components, metalloproteinases and their tissue inhibitors, growth factors, and cytokines in adhesion and peritoneal fibroblasts under normal and hypoxic conditions by reverse transcriptase/polymerase chain reaction analysis.
  
  
  RESULTS
  Compared to peritoneal fibroblasts, adhesion fibroblasts had a significant increase in the basal mRNA levels for collagen I, fibronectin, MMP-1, TIMP-1, TGF-beta 1, TGF-beta 2, and IL-10. Hypoxia resulted in a further increase in collagen 1, fibronectin, TIMP-1, TGF-beta 1, TGF-beta 2, IL-10, and IFN-gamma mRNA levels in both peritoneal and adhesion fibroblasts. The increase was more profound in adhesion fibroblasts.
  
  
  CONCLUSIONS
  Hypoxia induces molecular changes in both peritoneal and adhesion fibroblasts, creating a milieu that favors adhesion development. The effect of hypoxia was more profound on adhesion fibroblasts.
authors: Department of Obstetrics and Gynecology, Hutzel Hospital, Wayne State University, Detroit, Michigan, USA. g.saed@wayne.edu
authors: G M
authors: GM
authors: Saed
authors: W
authors: W
authors: Zhang
authors: M P
authors: MP
authors: Diamond
mesh_terms: D015687:Cell Hypoxia; D002478:Cells, Cultured; D003094:Collagen / Q000235:genetics; D016326:Extracellular Matrix Proteins / Q000032:analysis / Q000235:genetics*; D005260:Female; D005347:Fibroblasts / Q000166:cytology* / Q000473:pathology / Q000502:physiology; D005353:Fibronectins / Q000235:genetics; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics; D016753:Interleukin-10 / Q000235:genetics; D010535:Laparoscopy; D020781:Matrix Metalloproteinase 1 / Q000235:genetics; D017699:Pelvic Pain / Q000473:pathology* / Q000601:surgery; D010537:Peritoneum / Q000166:cytology* / Q000473:pathology / Q000502:physiology; D012016:Reference Values; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000267:Tissue Adhesions / Q000473:pathology* / Q000503:physiopathology; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000235:genetics; D014158:Transcription, Genetic; D016212:Transforming Growth Factor beta / Q000235:genetics
chemical_list: D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D019715:Tissue Inhibitor of Metalloproteinase-1; D016212:Transforming Growth Factor beta; D016753:Interleukin-10; D007371:Interferon-gamma; D003094:Collagen; D020781:Matrix Metalloproteinase 1