title: Local expression of epsilon germline gene transcripts and RNA for the epsilon heavy chain of IgE in the bronchial mucosa in atopic and nonatopic asthma.
abstract: BACKGROUND
  The demonstration of epsilon germline gene (Cepsilon) transcripts and mature mRNA for the epsilon heavy chain gene (Iepsilon) in the nasal mucosa suggested that IgE synthesis may occur in allergic rhinitis.
  
  
  OBJECTIVE
  In view of our previous demonstration of increases in IL-4 mRNA(+) cells in asthmatic subjects, we assessed whether local IgE synthesis may also be a feature of bronchial asthma.
  
  
  METHODS
  Fiberoptic bronchoscopic mucosa biopsy specimens were obtained from 9 atopic asthmatic subjects and 10 nonatopic normal (intrinsic) control subjects. To control for atopy, we also studied 9 nonatopic asthmatic subjects and 10 atopic nonasthmatic control subjects. Tissue was processed for immunohistochemistry for B cells (CD20) and in situ hybridization for Iepsilon and Cepsilon RNA(+) cells and IL-4 mRNA(+) cells.
  
  
  RESULTS
  B-cell numbers in the bronchial mucosa were similar for asthmatic subjects compared with control subjects, whereas significantly higher numbers of Iepsilon RNA(+) (P =.02 and P =.04, respectively), Cepsilon RNA(+) (P =.01 and P =.03, respectively), and IL-4 mRNA(+) (P =.001 and P =.001, respectively) cells were observed in atopic asthmatic subjects and nonatopic asthmatic subjects, respectively, but not in atopic control subjects compared with nonatopic control subjects. In asthmatic subjects there were significant correlations between Iepsilon RNA(+) cells (r = 0.54, P =.02) and Cepsilon RNA(+) cells (r = 0.48, P =.05) when compared with the number of IL-4 mRNA(+) cells.
  
  
  CONCLUSIONS
  Increases in Iepsilon and Cepsilon RNA(+) cells, but not B-cell numbers, in the bronchial mucosa provide evidence for local IgE synthesis in both atopic and nonatopic asthma. These changes appear to relate to asthma rather than atopy per se and, at least in part, may be under the regulation of IL-4.
authors: Allergy and Clinical Immunology, Imperial College School of Medicine at the National Heart and Lung Institute, Dovehouse St., London SW3 6LY, UK.
authors: S
authors: S
authors: Ying
authors: M
authors: M
authors: Humbert
authors: Q
authors: Q
authors: Meng
authors: R
authors: R
authors: Pfister
authors: G
authors: G
authors: Menz
authors: H J
authors: HJ
authors: Gould
authors: A B
authors: AB
authors: Kay
authors: S R
authors: SR
authors: Durham
mesh_terms: D000328:Adult; D000368:Aged; D001249:Asthma / Q000276:immunology*; D001980:Bronchi / Q000378:metabolism*; D005260:Female; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology*; D007073:Immunoglobulin E / Q000235:genetics*; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D015847:Interleukin-4 / Q000235:genetics; D008297:Male; D008875:Middle Aged; D009092:Mucous Membrane / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis*
chemical_list: D007143:Immunoglobulin Heavy Chains; D012333:RNA, Messenger; D015847:Interleukin-4; D007073:Immunoglobulin E