title: Acidic glycerol lipids of Trichomonas vaginalis and Tritrichomonas foetus.
abstract: The isolation and characterization of acidic lipids from both Trichomonas vaginalis and Tritrichomonas foetus have been carried out using radiolabeling, a combination of high performance liquid and thin layer chromatographic techniques, and mass spectrometry. Unique among the eukaryotes, these organisms produce phosphatidylglycerols and O-acyl phosphatidylglycerol-like compounds. In this study, the molecular weight distributions of the phosphatidylglycerols and acyl phosphatidylglycerols were determined by negative-ion liquid secondary ionization mass spectrometry (LSIMS) and the fatty acyl groups within each molecular species were assessed by collision-induced decomposition tandem mass spectrometry (CID MS/MS). Both species were found to contain primarily oleic acid in the sn-2 position. The lipids of T. vaginalis had approximately equal amounts of C16 and C18 in the sn-1 position, with varying degrees of unsaturation, especially in the C18 species. The T. foetus lipids had C18 almost exclusively, but also varied in the unsaturation. Other acidic lipids included inositol phosphosphingolipids and inositol diphosphosphingolipids.
authors: Department of Biochemistry, Boston University School of Medicine, MA 02118, USA.
authors: C E
authors: CE
authors: Costello
authors: D H
authors: DH
authors: Beach
authors: B N
authors: BN
authors: Singh
mesh_terms: D000818:Animals; D006017:Glycolipids / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification; D010712:Phosphatidic Acids / Q000737:chemistry; D010715:Phosphatidylglycerols / Q000737:chemistry*; D018629:Spectrometry, Mass, Secondary Ion / Q000379:methods; D014246:Trichomonas vaginalis / Q000737:chemistry*; D016835:Tritrichomonas foetus / Q000737:chemistry*
chemical_list: D006017:Glycolipids; D010712:Phosphatidic Acids; D010715:Phosphatidylglycerols; C015903:glycerolglycolipids; C021745:semilysobisphosphatidic acid