Molecular cloning of the rat TA1/LAT-1/CD98 light chain gene promoter.
The rat LAT-1 (L-amino acid transporter-1) gene is a CD98 light chain highly expressed in cancer and development. As an initial study of the molecular basis underlying regulation of its expression, we cloned 2 kb of the LAT-1 5' flanking region. Inverse RACE and primer extension methods were used to define the transcription initiation site at 80 bp upstream from the translational start site. Functional studies carried out in normal hepatic cells using constructs containing progressive 5' deletion from region -1958 to -185 showed 3-5-fold beta-galactosidase activities over control. The presence of an activator site(s) between -52 and -185 was indicated by low activities conferred by the construct spanning this region.
Diah
S K
SK
Division of Medical Oncology, George 312, Rhode Island Hospital/Brown Medical School, 593 Eddy Street, Providence, RI 02903, USA.
Padbury
J F
JF
Campbell
W A
WA
Britt
D
D
Thompson
N L
NL
D000818:Animals; D015703:Antigens, CD / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D001665:Binding Sites; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics*; D002460:Cell Line; D003001:Cloning, Molecular; D027261:Fusion Regulatory Protein-1; D005786:Gene Expression Regulation; D015698:Genomic Library; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D051381:Rats; D014162:Transfection
D015703:Antigens, CD; D002352:Carrier Proteins; D027261:Fusion Regulatory Protein-1