Isolation and characterization of the TERE1 gene, a gene down-regulated in transitional cell carcinoma of the bladder.
We have identified a novel cDNA product designated transitional epithelial response gene (TERE1), which was localized to chromosome 1p36. The TERE1 transcript (1.5 and 3.5 kb) is present in most normal human tissues including urothelium, but was reduced or absent in the majority of muscle invasive TCC tumors (22 out of 29 cases). The open reading frame encodes a protein of 338 amino acids (MW 36.8 KD). This protein is 57% homologous to a Drosophila protein called heix. We have shown by Western blotting and immuno-histochemistry with a polyclonal antibody to a specific TERE1 peptide, reduced or absent staining in muscle invasive tumors. Transfection of a sense TERE1 construct resulted in an 80-90% inhibition of cellular proliferation in two TCC cell lines and a lack of aneuploidy in the TERE1-transduced J82 cell line. These data suggest a potential role for this gene product in the progression of bladder cancer.
McGarvey
T W
TW
Department of Surgery, Division of Urology, University of Pennsylvania Medical Center and Veterans Administration Medical Center, Philadelphia, Pennsylvania, PA 19104, USA.
Nguyen
T
T
Tomaszewski
J E
JE
Monson
F C
FC
Malkowicz
S B
SB
D000328:Adult; D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D002295:Carcinoma, Transitional Cell / Q000235:genetics* / Q000378:metabolism; D017931:DNA Primers / Q000737:chemistry; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification*; D004122:Dimethylallyltranstransferase; D015536:Down-Regulation; D005434:Flow Cytometry; D005822:Genetic Vectors; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008969:Molecular Sequence Data; D018807:Polymorphism, Single-Stranded Conformational; D011506:Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D012190:Retroviridae / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000378:metabolism
D017931:DNA Primers; D018076:DNA, Complementary; D011506:Proteins; D004122:Dimethylallyltranstransferase; C425464:UBIAD1 protein, human