An in vitro approach to the evaluation of the cross talk between intestinal epithelium and macrophages.
The intestinal epithelium acts as a mucosal barrier by varying their signals to immune cells within the intestine. To observe the cross talk between intestinal epithelium and macrophages, we establish a Caco-2-THP-1 co-culture system. Using this co-culture system, we suggested that paracrine factors of intestinal epithelium increased the phagocytic capacity of intestinal monocytes/macrophages to be ready for immune and inflammatory responses.
Kanzato
H
H
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan.
Manabe
M
M
Shimizu
M
M
D018938:Caco-2 Cells; D002450:Cell Communication; D002460:Cell Line; D018920:Coculture Techniques; D017077:Culture Media, Conditioned; D017097:Electric Impedance; D004847:Epithelial Cells / Q000502:physiology; D006801:Humans; D007422:Intestines / Q000502:physiology*; D008262:Macrophage Activation; D008264:Macrophages / Q000502:physiology*; D010587:Phagocytosis
D017077:Culture Media, Conditioned