title: Spectrochemical investigations in molecularly organized solvent media: evaluation of pyridinium chloride as a selective fluorescence quenching agent of polycyclic aromatic hydrocarbons in aqueous carboxylate-terminated poly(amido) amine dendrimers and anionic micelles.
abstract: The ability of pyridinium chloride (PC) to selectively quench alternant as opposed to nonaltemant polycyclic aromatic hydrocarbons (PAHs) in organized media is examined. PC was previously shown to be a selective quenching agent of alternant PAHs in neat polar solvents. Carboxylate-terminated poly(amido) amine (PAMAM-CT) dendrimers and anionic surfactants--sodium dodecanoate (SD), sodium octanoate (SO), and sodium dodecylsulfate (SDS)--were chosen as the solubilizing media for this study. Selective quenching of alternant PAHs is observed in the presence of the SDS and SO micelles. However, the extent of PAH quenching in SO is significantly reduced compared to PAHs dissolved in either water or SDS micelles. In the case of the smaller generation 4.5 (G4.5) PAMAM-CT dendrimers, PC was prevented from quenching both alternant and nonalternant PAHs to any appreciable extent. The dendrimer is able to "protect" the PAHs from the PC quencher that resides at the dendrimer surface. Both, SD and G5.5 PAMAM-CT precipitated out of solution with the addition of PC. Differences between traditional micelles and "unimolecular micelle" dendrimers were also examined. These studies further confirm that the PAHs did not reside in the "analogous" palisade region of the dendrimers as they do in micelles. The PAHs must reside in the outermost branches of the dendrimer, but sufficiently far enough away from the charged surface groups, where PC associated, to prevent fluorescence quenching. This work further illustrates the differences between "unimolecular micelle" dendrimers and traditional micelles.
authors: Department of Chemistry, University of Missouri-Columbia, 65211, USA.
authors: D A
authors: DA
authors: Wade
authors: C
authors: C
authors: Mao
authors: A C
authors: AC
authors: Hollenbeck
authors: S A
authors: SA
authors: Tucker
mesh_terms: D000588:Amines; D000838:Anions; D004785:Environmental Pollutants / Q000032:analysis*; D007202:Indicators and Reagents; D008823:Micelles; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis*; D011726:Pyridinium Compounds; D015203:Reproducibility of Results; D012997:Solvents; D013050:Spectrometry, Fluorescence / Q000379:methods; D013053:Spectrophotometry / Q000379:methods
chemical_list: D000588:Amines; D000838:Anions; D004785:Environmental Pollutants; D007202:Indicators and Reagents; D008823:Micelles; D011084:Polycyclic Aromatic Hydrocarbons; D011726:Pyridinium Compounds; D012997:Solvents