title: Identification of dimethylarsinous and monomethylarsonous acids in human urine of the arsenic-affected areas in West Bengal, India.
abstract: A speciation technique for arsenic has been developed using an anion-exchange high-performance liquid chromatography/inductively coupled argon plasma mass spectrometer (HPLC/ICP MS). Under optimized conditions, eight arsenic species [arsenocholine, arsenobetaine, dimethylarsinic acid (DMA(V)), dimethylarsinous acid (DMA(III)), monomethylarsonic acid (MMA(V)), monomethylarsonous acid (MMA(III)), arsenite (As(III)), and arsenate (As(V))] can be separated with isocratic elution within 10 min. The detection limit of arsenic compounds was 0.14-0.33 microg/L. To validate the method, Standard Reference Material in freeze-dried urine, SRM-2670, containing both normal and elevated levels of arsenic was analyzed. The method was applied to determine arsenic species in urine samples from three arsenic-affected districts of West Bengal, India. Both DMA(III) and MMA(III) were detected directly (i.e., without any prechemical treatment) for the first time in the urine of some humans exposed to inorganic arsenic through their drinking water. Of 428 subjects, MMA(III) was found in 48% and DMA(III) in 72%. Our results indicate the following. (1) Since MMA(III) and DMA(III) are more toxic than inorganic arsenic, it is essential to re-evaluate the hypothesis that methylation is the detoxification pathway for inorganic arsenic. (2) Since MMA(V) reductase with glutathione (GSH) is responsible for conversion of MMA(V) to MMA(III) in vivo, is DMA(V) reductase with GSH responsible for conversion of DMA(V) to DMA(III) in vivo? (3) Since DMA(III) forms iron-dependent reactive oxygen species (ROS) which causes DNA damage in vivo, DMA(III) may be responsible for arsenic carcinogenesis in human.
authors: Faculty of Pharmaceutical Sciences, Chiba University, Inage, Chiba 263-8522, Japan.
authors: B K
authors: BK
authors: Mandal
authors: Y
authors: Y
authors: Ogra
authors: K T
authors: KT
authors: Suzuki
mesh_terms: D001151:Arsenic / Q000032:analysis*; D002101:Cacodylic Acid / Q000652:urine*; D002851:Chromatography, High Pressure Liquid; D004785:Environmental Pollutants / Q000032:analysis* / Q000652:urine; D006801:Humans; D007194:India; D013058:Mass Spectrometry; D009942:Organometallic Compounds / Q000652:urine*; D012680:Sensitivity and Specificity
chemical_list: D004785:Environmental Pollutants; D009942:Organometallic Compounds; C406082:monomethylarsonous acid; D002101:Cacodylic Acid; D001151:Arsenic