A physical map of the Mycoplasma agalactiae strain PG2 genome.
We have constructed a physical map of the Mycoplasma agalactiae strain PG2 chromosome analyzing it by pulsed field gel electrophoresis in a contour-clamped homogeneous electric-field system. We mapped 33 cleavage sites generated with SmaI, XhoI, SalI, EclXI and BsiWI restriction endonucleases using double digestions, one- and two-dimensional pulsed electrophoresis, cross-hybridization and linking clones. We have also mapped the loci of some genes by Southern hybridization.
Tola
S
S
Istituto Zooprofilattico Sperimentale della Sardegna G. Pegreffi, 07100 Sassari, Italy. sebastiana.tola@tin.it
Idini
G
G
Rocchigiani
A M
AM
Rocca
S
S
Manunta
D
D
Leori
G
G
D015139:Blotting, Southern; D002874:Chromosome Mapping; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D016521:Electrophoresis, Gel, Pulsed-Field; D016680:Genome, Bacterial; D009174:Mycoplasma / Q000235:genetics*; D015183:Restriction Mapping
C114636:endodeoxyribonuclease Ecl18kI; C025651:CCCGGG-specific type II deoxyribonucleases; C034510:CTCGAG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific; C029792:GTCGAC-specific type II deoxyribonucleases