Late temporal gene expression from the human cytomegalovirus pp28US (UL99) promoter when integrated into the host cell chromosome.
Toward understanding the temporal regulation of human cytomegalovirus (HCMV) late genes, we studied the regulation of the late gene promoter (pp28US, UL99) when outside the context of the viral genome and its response to the immediate early (IE) proteins. Expression of the luciferase reporter gene, regulated by the pp28US promoter, was synchronous with that of the endogenous viral pp28 gene, independently of whether the reporter was episomal or integrated into the glioblastoma cell line U373MG. Cotransfection of the reporter with expression vectors for each of the three major IE genes, IE72, IE86 and IE55, indicated that only IE86 transactivated the pp28US promoter. However, the magnitude of the promoter activation upon HCMV infection suggested that additional factors are also required for higher promoter activity. The promoter activation was specific to HCMV, as herpes simplex virus type 1 infection did not induce luciferase expression.
Wu
Jun
J
Signal Research Division of Celgene, 5555 Oberlin Drive, San Diego, CA 92121, USA1.
O'Neill
Joseph
J
Signal Research Division of Celgene, 5555 Oberlin Drive, San Diego, CA 92121, USA1.
Barbosa
Miguel S
MS
Signal Research Division of Celgene, 5555 Oberlin Drive, San Diego, CA 92121, USA1.
D002875:Chromosomes; D003587:Cytomegalovirus / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity; D004261:DNA Replication; D004279:DNA, Viral / Q000096:biosynthesis; D015870:Gene Expression; D015967:Gene Expression Regulation, Viral; D017930:Genes, Reporter; D006801:Humans; D017874:Immediate-Early Proteins / Q000378:metabolism; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D008562:Membrane Glycoproteins; D010750:Phosphoproteins / Q000235:genetics*; D011401:Promoter Regions, Genetic; D015534:Trans-Activators; D015533:Transcriptional Activation; D014407:Tumor Cells, Cultured; D014759:Viral Envelope Proteins; D014764:Viral Proteins / Q000235:genetics*; D016662:Virus Integration
D004279:DNA, Viral; C069186:IE2 protein, Cytomegalovirus; D017874:Immediate-Early Proteins; D008562:Membrane Glycoproteins; D010750:Phosphoproteins; D015534:Trans-Activators; C077406:UL115 protein, Human herpesvirus 5; D014759:Viral Envelope Proteins; D014764:Viral Proteins; C071440:glycoprotein H, Cytomegalovirus; C499358:glycoprotein H, Human cytomegalovirus; C439068:glycoprotein O, cytomegalovirus; C070746:human cytomegalovirus protein pp28; D008156:Luciferases