Pulmonary surfactant protein SP-B is significantly more immunoreactive in anionic than in zwitterionic bilayers.
Binding of polyclonal and monoclonal antibodies, quantitated by enzyme-linked immunosorbent assay, to porcine SP-B reconstituted in different phospholipid bilayers has been used to assess differences in protein structure due to lipid-protein interactions. SP-B bound significantly more antibodies when it was reconstituted in bilayers made of anionic phospholipids (phosphatidic acid, cardiolipin, phosphatidylglycerol, phosphatidylinositol or phosphatidylserine) than in zwitterionic bilayers (phosphatidylcholine, phosphatidylcholine/cholesterol, or phosphatidylethanolamine) or in fatty acid micelles (made of salts of palmitic or stearic acids). These differences in immunoreactivity can be important in the development of quantitation methods for SP-B in clinical samples based on immunological techniques.
Oviedo
J M
JM
Departamento Bioquímica y Biología Molecular I, Facultad Biología, Universidad Complutense, 28040 Madrid, Spain.
Casals
C
C
Pérez-Gil
J
J
D000818:Animals; D000838:Anions / Q000737:chemistry / Q000378:metabolism; D000911:Antibodies, Monoclonal / Q000276:immunology; D000941:Antigens / Q000276:immunology* / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D007106:Immune Sera / Q000276:immunology; D008051:Lipid Bilayers / Q000737:chemistry* / Q000378:metabolism*; D008823:Micelles; D019308:Palmitic Acid / Q000378:metabolism; D010743:Phospholipids / Q000737:chemistry / Q000378:metabolism; D011510:Proteolipids / Q000276:immunology* / Q000378:metabolism*; D011663:Pulmonary Surfactants / Q000276:immunology* / Q000378:metabolism*; D055672:Static Electricity; D013229:Stearic Acids / Q000378:metabolism; D013552:Swine
D000838:Anions; D000911:Antibodies, Monoclonal; D000941:Antigens; D007106:Immune Sera; D008051:Lipid Bilayers; D008823:Micelles; D010743:Phospholipids; D011510:Proteolipids; D011663:Pulmonary Surfactants; D013229:Stearic Acids; D019308:Palmitic Acid; C031183:stearic acid