Application of a Y-STR-pentaplex PCR (DYS19, DYS389I and II, DYS390 and DYS393) to sexual assault cases.
A survey of 89 random rape cases including 166 semen traces, according to the prostate-specific-antigen test, showed the need for a more efficient method of DNA-profiling. No male autosomal STR-profile could be detected, after differential lysis, in 35% of the 84 samples usually containing high amounts of the victim's cells, i.e. vaginal/anal washings and knickers. A Y-STR-pentaplex was applied to the stored sperm fraction of 50 unsolved semen traces, with success in 48% of them. The chance of success depends of the amount of total DNA available and of the quality of the extracted DNA.
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National Institute for Criminalistics and Criminology, Ch. De Vilvorde 98-100, B-1120, Brussels, Belgium.
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D001530:Belgium; D016172:DNA Fingerprinting; D005260:Female; D005554:Forensic Medicine / Q000379:methods; D005787:Gene Frequency; D006239:Haplotypes; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D011902:Rape; D012661:Semen; D012680:Sensitivity and Specificity; D020080:Tandem Repeat Sequences / Q000235:genetics*