A role for actin, Cdc1p, and Myo2p in the inheritance of late Golgi elements in Saccharomyces cerevisiae.
In Saccharomyces cerevisiae, Golgi elements are present in the bud very early in the cell cycle. We have analyzed this Golgi inheritance process using fluorescence microscopy and genetics. In rapidly growing cells, late Golgi elements show an actin-dependent concentration at sites of polarized growth. Late Golgi elements are apparently transported into the bud along actin cables and are also retained in the bud by a mechanism that may involve actin. A visual screen for mutants defective in the inheritance of late Golgi elements yielded multiple alleles of CDC1. Mutations in CDC1 severely depolarize the actin cytoskeleton, and these mutations prevent late Golgi elements from being retained in the bud. The efficient localization of late Golgi elements to the bud requires the type V myosin Myo2p, further suggesting that actin plays a role in Golgi inheritance. Surprisingly, early and late Golgi elements are inherited by different pathways, with early Golgi elements localizing to the bud in a Cdc1p- and Myo2p-independent manner. We propose that early Golgi elements arise from ER membranes that are present in the bud. These two pathways of Golgi inheritance in S. cerevisiae resemble Golgi inheritance pathways in vertebrate cells.
Rossanese
O W
OW
Department of Molecular Genetics and Cell Biology, The University of Chicago, Illinois 60637, USA.
Reinke
C A
CA
Bevis
B J
BJ
Hammond
A T
AT
Sears
I B
IB
O'Connor
J
J
Glick
B S
BS
D000199:Actins / Q000502:physiology*; D015415:Biomarkers; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000494:pharmacology; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D004352:Drug Resistance, Microbial; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D006056:Golgi Apparatus / Q000502:physiology*; D049452:Green Fluorescent Proteins; D020662:Guanine Nucleotide Exchange Factors; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008565:Membrane Proteins; D016296:Mutagenesis; D018995:Myosin Heavy Chains; D024462:Myosin Type II; D024701:Myosin Type V; D009218:Myosins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D010641:Phenotype; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism / Q000502:physiology; D029701:Saccharomyces cerevisiae Proteins; D029702:Schizosaccharomyces pombe Proteins; D013844:Thiazoles / Q000494:pharmacology; D053778:Thiazolidines; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
D000199:Actins; D015415:Biomarkers; D019086:Bridged Bicyclo Compounds, Heterocyclic; C090459:CDC1 protein, S cerevisiae; D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D005656:Fungal Proteins; D020662:Guanine Nucleotide Exchange Factors; D008164:Luminescent Proteins; C077512:MYO2 protein, S cerevisiae; C434211:MYO2 protein, S pombe; D008565:Membrane Proteins; C105592:RIC1 protein, S cerevisiae; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; D029702:Schizosaccharomyces pombe Proteins; C066755:Sec7 guanine nucleotide exchange factors; D013844:Thiazoles; D053778:Thiazolidines; D014157:Transcription Factors; D049452:Green Fluorescent Proteins; D024462:Myosin Type II; D024701:Myosin Type V; D018995:Myosin Heavy Chains; D009218:Myosins; C037067:latrunculin A