Indirect micromanipulation of single molecules in water-in-oil emulsion.
Based on real-time observation and micromanipulation, analytical methods for single DNA molecules have been under development for some time. Precise manipulation, however, is still difficult because single molecules are too small for conventional techniques. We have developed a chemical reaction system that uses water droplets in oil as containers of materials. The water droplets can be manipulated by optical force. The manipulation of the water droplets permits the fusion of two selected droplets. This process corresponds to mixing of different samples. We designate this system as "w/o (water-in-oil emulsion) microreactor system", and each droplet can be thought of as a "microreactor". In this system, single molecules can be manipulated readily, as a molecule can be contained in a microm-sized microreactor. The microreactor utilizes extremely small quantities of samples, therefore, reactions are rapid, as diffusion times in the microreactor are very short. The manipulation technique of the microreactors based on optical force has been applied to induce fusion between microreactors loaded with DNA and YOYO, a fluorescent dye that binds to DNA. This fusion induced a rapid binding of YOYO.
Katsura
S
S
Department of Ecological Engineering, Toyohashi University of Technology, Aichi, Japan. katsura@eco.tut.ac.jp
Yamaguchi
A
A
Inami
H
H
Matsuura
S
S
Hirano
K
K
Mizuno
A
A
D001583:Benzoxazoles / Q000378:metabolism; D004271:DNA, Fungal / Q000378:metabolism; D004058:Diffusion; D004655:Emulsions; D004867:Equipment Design; D005229:Fatty Acids, Monounsaturated; D005456:Fluorescent Dyes / Q000378:metabolism; D007091:Image Processing, Computer-Assisted; D007834:Lasers; D008832:Microchemistry / Q000295:instrumentation / Q000379:methods*; D008846:Micromanipulation / Q000295:instrumentation / Q000379:methods*; D010784:Photomicrography / Q000295:instrumentation; D010938:Plant Oils; D011806:Quinolinium Compounds / Q000378:metabolism; D000074262:Rapeseed Oil; D012441:Saccharomyces cerevisiae / Q000737:chemistry; D014743:Videotape Recording; D014867:Water
D001583:Benzoxazoles; D004271:DNA, Fungal; D004655:Emulsions; D005229:Fatty Acids, Monounsaturated; D005456:Fluorescent Dyes; D010938:Plant Oils; D011806:Quinolinium Compounds; D000074262:Rapeseed Oil; D014867:Water; C075296:1,1'-((4,4,7,7-tetramethyl)-4,7-diazaundecamethylene)bis-4-(3-methyl-2,3-dihydro(benzo-1,3-oxazole)-2-methylidene)quinolinium