Effects of synthetic craniofacial materials on cerebral microcirculation.
Four groups were studied to look at effects of synthetic materials on the pial vasculature. Using Sprague-Dawley rats, an open pial window approach was used in which there was a control group, a hydroxyapatite cement group mixed with sodium phosphate, a methylmethacrylate slow-set, and a methylmethacrylate fast-set group. There were 10 animals with 20 vessels studied within each group. The permeability leakage outside the vessel was evaluated to determine the vascular albumin leakage, and the number of rolling and adherent leukocytes was studied within each group. It was seen that the control group was significantly different compared with the fast-set methylmethacrylate group during a 2-hour period in regard to the percentage leakage, as well as a number of rolling and adherent leukocytes. This is one of the first studies to demonstrate the effects of synthetic craniofacial materials on the underlying pial vasculature.
Barone
C M
CM
Division of Plastic Surgery, University of Missouri, One Hospital Drive, Columbia, MO 65212, USA.
Jimenez
D F
DF
Beckert
B W
BW
Clapper
A T
AT
D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D018786:Bone Substitutes / Q000494:pharmacology*; D002199:Capillary Permeability / Q000187:drug effects; D002448:Cell Adhesion / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D002560:Cerebrovascular Circulation / Q000187:drug effects*; D003399:Craniotomy; D017886:Durapatite / Q000494:pharmacology; D005119:Extravasation of Diagnostic and Therapeutic Materials / Q000503:physiopathology; D005260:Female; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D007962:Leukocytes / Q000187:drug effects; D020366:Methylmethacrylate / Q000494:pharmacology; D008833:Microcirculation / Q000187:drug effects; D008856:Microscopy, Fluorescence; D023421:Models, Animal; D010710:Phosphates / Q000494:pharmacology; D010841:Pia Mater / Q000098:blood supply; D051381:Rats; D017207:Rats, Sprague-Dawley; D012709:Serum Albumin / Q000187:drug effects
D001672:Biocompatible Materials; D018786:Bone Substitutes; D005456:Fluorescent Dyes; D010710:Phosphates; D012709:Serum Albumin; D020366:Methylmethacrylate; D017886:Durapatite; D016650:Fluorescein-5-isothiocyanate; C018279:sodium phosphate