Plasmid DNA encoding caprine interferon gamma inhibits antibody response to caprine arthritis-encephalitis virus (CAEV) surface protein encoded by a co-administered plasmid expressing CAEV env and tat genes.
This study characterized immune responses of Saanen goats co-immunized with pUC18 based plasmids expressing caprine arthritis-encephalitis virus (CAEV) rev-env (pENV) or tat-rev-env (pTAT-ENV) and a second plasmid encoding caprine interferon gamma (IFNgamma). A previous study reported that immunization with pENV or pTAT-ENV induces IgG2 biased antibody responses to plasmid encoded CAEV surface envelope protein (SU). We show here that regional lymph nodes (LN) of pENV and pTAT-ENV immunized goats contain a dominant subset of SU activated IFNgamma+ Th1 lymphocytes. Co-immunization with pENV or pTAT-ENV and a caprine IFNgamma cDNA expression plasmid (pcIFNgamma) did not potentiate activation of SU responsive Th1 lymphocytes. However, the antibody response to SU encoded by pTAT-ENV was inhibited by co-immunization with pcIFNgamma. Results indicate that synergistic effects of CAEV Tat and IFNgamma suppress the primary adaptive B cell response to plasmid encoded SU.
Cheevers
W P
WP
Department of Veterinary Microbiology and Pathology, Washington State University, 99164-7040, Pullman, WA, USA. cheevers@vetmed.wsu.edu
Beyer
J C
JC
Hötzel
I
I
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D016088:Arthritis-Encephalitis Virus, Caprine / Q000235:genetics* / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology; D015870:Gene Expression; D015752:Genes, env; D015756:Genes, tat; D015511:Goat Diseases / Q000235:genetics / Q000276:immunology; D006041:Goats; D007371:Interferon-gamma / Q000235:genetics*; D016180:Lentivirus Infections / Q000235:genetics / Q000276:immunology; D008213:Lymphocyte Activation; D010957:Plasmids / Q000235:genetics; D019444:Vaccines, DNA / Q000235:genetics / Q000494:pharmacology; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology; D014765:Viral Vaccines / Q000235:genetics / Q000494:pharmacology
D000914:Antibodies, Viral; D019444:Vaccines, DNA; D014759:Viral Envelope Proteins; D014765:Viral Vaccines; D007371:Interferon-gamma