Natural zeolite clinoptilolite increases the concentrations of sphingoid bases in the yeast Yarrowia lipolytica.
In the present paper, we studied the effect of natural zeolite clinoptilolite on sphingolipid metabolism in the yeast Yarrowia lipolytica. We also investigated if zeolite addition had any impact on cell shape and size, as well as on the pH alterations during the culture growth. High performance liquid chromatography analysis of sphingoid bases obtained by acid hydrolysis of complex sphingolipids from Y. lipolytica showed that their concentrations markedly rose upon the zeolite addition. The largest increase among the identified molecular species of sphingoid bases was seen in C18 phytosphingosine, whose levels rose 6.2-fold and 22.3-fold after culturing cells for 24 and 36 hours respectively in the presence of finely ground zeolite. pH measurements of the culture medium showed a similarity between pH profiles of control and zeolite-supplemented cells, suggesting that ion-exchange capacity was not probably responsible for the observed change in sphingolipid metabolism. Scanning electron microscopy revealed that zeolite affected cell size and shape. Y. lipolytica cells grown in the absence of zeolite were oval-shaped with an average cell size of 0.7-2.7 microns, whereas when cultured with zeolite, they were round-shaped and larger, having an average cell size of 1.3-2.9 microns.
Bauman
M
M
Department of Chemistry and Biochemistry, School of Medicine, University of Zagreb, Salata 3, HR-10000 Zagreb, Croatia.
Mesarić
M
M
Ribar
S
S
Marić
V
V
Tudja
M
M
D003470:Culture Media; D006863:Hydrogen-Ion Concentration; D004718:Saccharomycetales / Q000737:chemistry* / Q000166:cytology / Q000254:growth & development; D013107:Sphingolipids / Q000032:analysis*; D017641:Zeolites / Q000494:pharmacology*
D003470:Culture Media; D013107:Sphingolipids; C083175:clinoptilolite; D017641:Zeolites