Proadrenomedullin N-terminal 20 peptide (PAMP) inhibits food intake and gastric emptying in mice.
We found that proadrenomedullin N-terminal 20 peptide (PAMP) decreased dose-dependently (3-30 nmol/mouse) food intake after intra-third cerebroventricular administration in fasted ddY mice. Gastric emptying also was delayed after central injection of PAMP. In our previous study, PAMP was demonstrated to elicit hyperglycemia via bombesin (BN) receptor. Then, we examined whether the effects of PAMP on feeding and gastric emptying were induced through BN receptor. Surprisingly, PAMP-induced reductions in feeding and gastric emptying rate were not blocked by a BN antagonist, [D-Phe(6), Leu-NHEt(13), des-Met(14)]-BN (6-14). PAMP suppressed feeding in mice lacking gastrin-releasing peptide receptor or BN receptor subtype-3. These results indicate that centrally administered PAMP inhibits food intake, involving the delayed gastric emptying, not through BN receptors but through selective PAMP receptor.
Ohinata
K
K
Department of Functional Food Resources, Research Institute for Food Science, Kyoto University, Gokasho Uji, 611-0011, Kyoto, Japan.
Inui
A
A
Asakawa
A
A
Wada
K
K
Wada
E
E
Yoshikawa
M
M
D053607:Adrenomedullin; D000818:Animals; D004305:Dose-Response Relationship, Drug; D002149:Energy Intake / Q000187:drug effects*; D005746:Gastric Emptying / Q000187:drug effects*; D006801:Humans; D007276:Injections, Intraventricular; D051379:Mice; D018345:Mice, Knockout; D010446:Peptide Fragments / Q000008:administration & dosage / Q000494:pharmacology*; D010455:Peptides; D011506:Proteins / Q000008:administration & dosage / Q000494:pharmacology*; D018004:Receptors, Bombesin / Q000037:antagonists & inhibitors / Q000235:genetics
D010446:Peptide Fragments; D010455:Peptides; D011506:Proteins; D018004:Receptors, Bombesin; D053607:Adrenomedullin