Alteration of Fas/FasL expression of oral squamous cell carcinoma cell line Tca83 induced by retinoic acid.
OBJECTIVE
To elucidate the chemosensitivity of human tongue squamous cell carcinoma Tca83 cell line to all-trans retinoid acid (RA) and to determine the potential genetic factors mediating this sensitivity.


METHODS
RA in the amounts of 0.1, 1, and 10 microMol/L were used to treat human cell line Tca83 in vitro. Cell apoptosis as well as Fas/FasL expression were analyzed by TUNEL, RT-PCR, and immunohistochemistry approaches.


RESULTS
RA in the amounts of 1 and 10 microMol/L RA began to inhibit cell proliferation at day 3 and had induced cell apoptosis on day 5. Both Fas and FasL could be detected in the treated and untreated cells. RA upregulated Fas mRNA and protein expression at day 5. RA had no obvious effect on FasL expression.


CONCLUSIONS
The results suggest that apoptosis induced by RA may result from the enhancement of Fas gene transcription and translation.
Guan
W Q
WQ
Department of Stomatology, Union Hospital, Fuzhou City, P.R. China.
Yu
S F
SF
Gao
Y
Y
D000954:Antigens, Surface / Q000187:drug effects* / Q000235:genetics; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects* / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000276:immunology*; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004396:Coloring Agents; D053222:Fas Ligand Protein; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D008024:Ligands; D008562:Membrane Glycoproteins / Q000187:drug effects* / Q000235:genetics; D014176:Protein Biosynthesis / Q000187:drug effects; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013778:Tetrazolium Salts; D013844:Thiazoles; D013997:Time Factors; D014062:Tongue Neoplasms / Q000235:genetics / Q000276:immunology*; D014158:Transcription, Genetic / Q000187:drug effects; D014212:Tretinoin / Q000008:administration & dosage / Q000494:pharmacology*; D014407:Tumor Cells, Cultured; D015854:Up-Regulation / Q000187:drug effects; D019014:fas Receptor / Q000187:drug effects* / Q000235:genetics
D000954:Antigens, Surface; D000970:Antineoplastic Agents; D004396:Coloring Agents; C505661:FASLG protein, human; D053222:Fas Ligand Protein; D008024:Ligands; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; D013778:Tetrazolium Salts; D013844:Thiazoles; D019014:fas Receptor; D014212:Tretinoin; C022616:thiazolyl blue