The 14-3-3 protein as a vaccine candidate against schistosomiasis.
We have previously reported on the cloning of the 14-3-3 protein of Schistosoma mansoni. Here, we evaluate the potential use of this protein as a vaccine candidate against infection by S. mansoni. Sm14-3-3 was expressed and purified either as a free protein or as a fusion protein to SjGST or MBP. Sera from mice infected with S. mansoni recognized both SjGST and 14-3-3, indicating that antibodies against these two proteins are induced in the course of the natural infection. Furthermore, mice immunized with either 14-3-3, GST or 14-3-3-GST, reacted with cercaria lysate. A cellular immune response was also detected, particularly in mice immunized with 14-3-3-GST. With respect to the effect on biological functions, antibodies to 14-3-3 and 14-3-3-GST caused 23-32% complement-mediated cytotoxcity of S. mansoni schistosomula compared to only 10-11% induced by either normal mouse serum, or GST alone. In challenge infection with S. mansoni, immunization with 14-3-3, either as a fusion protein or as a free protein, led to protection ranging from 25-46%, as determined by reduction of adult worm burden, while SjGST alone elicited only 0-8% protection and MBP alone did not elicit any protection.
Schechtman
D
D
Department of Immunology, Weizmann Institute of Science, Rehovot, Israel.
Tarrab-Hazdai
R
R
Arnon
R
R
D048948:14-3-3 Proteins; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D000947:Antigens, Helminth / Q000008:administration & dosage* / Q000276:immunology; D005982:Glutathione Transferase / Q000276:immunology; D015801:Helminth Proteins / Q000008:administration & dosage* / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D011993:Recombinant Fusion Proteins / Q000008:administration & dosage; D011994:Recombinant Proteins / Q000008:administration & dosage; D012550:Schistosoma mansoni / Q000276:immunology / Q000302:isolation & purification; D012552:Schistosomiasis / Q000276:immunology / Q000469:parasitology / Q000517:prevention & control*; D014446:Tyrosine 3-Monooxygenase / Q000008:administration & dosage / Q000276:immunology; D014611:Vaccination; D014612:Vaccines / Q000008:administration & dosage*; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000276:immunology
D048948:14-3-3 Proteins; D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D015801:Helminth Proteins; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D014612:Vaccines; D014614:Vaccines, Synthetic; D014446:Tyrosine 3-Monooxygenase; D005982:Glutathione Transferase