Analysis of the epitopes on staphylococcal enterotoxin A responsible for emetic activity.
To identify which region of staphylococcal enterotoxin A (SEA) is responsible for the emetic activity, twelve synthetic peptides corresponding to the entire SEA amino acid sequence and their respective anti-peptide antibodies were prepared and tested. The anti-peptide antibodies were tested for neutralization of SEA-induced emesis in Suncus murinus (Shrew mouse). The results indicate that SEA-induced emesis was neutralized by the mixture of three anti-peptide antibodies to A-7 (corresponding to amino acid residues 121-140), A-8 (141-160) and A-9 (160-180). These findings suggest that the regions corresponding to residues 121-180 may be the epitopes responsible for the emetic activity of SEA.
Hu
D L
DL
Department of Veterinary Microbiology, Faculty of Agriculture, Iwate University, Morioka, Japan.
Omoe
K
K
Saleh
M H
MH
Ono
K
K
Sugii
S
S
Nakane
A
A
Shinagawa
K
K
D000818:Animals; D000906:Antibodies / Q000276:immunology / Q000494:pharmacology; D004768:Enterotoxins / Q000276:immunology*; D000939:Epitopes / Q000032:analysis*; D005260:Female; D008297:Male; D012376:Rodent Diseases / Q000139:chemically induced / Q000276:immunology* / Q000382:microbiology; D012377:Rodentia; D013210:Staphylococcus / Q000737:chemistry / Q000276:immunology*; D014839:Vomiting / Q000139:chemically induced* / Q000276:immunology / Q000628:therapy
D000906:Antibodies; D004768:Enterotoxins; D000939:Epitopes; C026066:enterotoxin A, Staphylococcal