The use of counterflow centrifugation to enrich gonadotropes and somatotropes.
Counterflow centrifugation produces populations of gonadotropes or growth hormone (GH) cells enriched to 90% in a Beckman elutriator. The pituitary populations are first separated by size into three fractions applying different flow rates, stimulated with either gonadotropin-releasing hormone (GnRH) to enlarge the gonadotropes or growth hormone-releasing hormone (GHRH) to enlarge the somatotropes for 3 hr. The fractions are re-eluted, first at the original flow rates and then at higher flow rates to separate enlarged gonadotropes or somatotropes. Most other cell types are reduced to less than 5%. However, co-storage of GH and gonadotropin antigens is seen in either population. Enriched gonadotropes or somatotropes can be used in studies of proliferation, autocrine or paracrine regulation, or ion channel functions.(J Histochem Cytochem 49:663-664, 2001)
Childs
G V
GV
Department of Anatomy, University of Arkansas for Medical Sciences, 4301 West Markham Street, Little Rock, AR 72205-7199, USA.
Unabia
G
G
D000818:Animals; D002469:Cell Separation / Q000379:methods; D002498:Centrifugation; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000378:metabolism*; D013007:Growth Hormone-Releasing Hormone / Q000378:metabolism*; D008297:Male; D010902:Pituitary Gland / Q000166:cytology* / Q000378:metabolism; D051381:Rats
D007987:Gonadotropin-Releasing Hormone; D013007:Growth Hormone-Releasing Hormone