An Arabidopsis thaliana protein interacts with a movement protein of Turnip crinkle virus in yeast cells and in vitro.
Plant virus movement proteins bind host components to promote virus movement from initially infected cells to neighbouring cells. In this study, cDNA clones encoding p8 and p9, two small proteins required for the movement of Turnip crinkle virus, were used as 'bait' in a yeast two-hybrid system to screen an Arabidopsis thaliana cDNA library for interactive proteins. One A. thaliana clone was identified that encodes a protein, designated Atp8, which interacted with p8 in yeast cells and in vitro. The apparent full-length of Atp8 mRNA was sequenced and shown to encode a protein with two possible transmembrane helices, several potential phosphorylation sites and two 'RGD' sequences.
Lin
Bing
B
Department of Plant Pathology, Kansas State University, Manhattan, KS 66506-5502, USA1.
Heaton
Louis A
LA
Department of Plant Pathology, Kansas State University, Manhattan, KS 66506-5502, USA1.
D000595:Amino Acid Sequence; D017360:Arabidopsis; D029681:Arabidopsis Proteins; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009842:Oligopeptides / Q000378:metabolism; D010641:Phenotype; D010940:Plant Proteins / Q000378:metabolism*; D053830:Plant Viral Movement Proteins; D012150:Polymorphism, Restriction Fragment Length; D012441:Saccharomyces cerevisiae; D019183:Tombusviridae / Q000378:metabolism*; D014764:Viral Proteins / Q000378:metabolism*
C426064:ATP8 protein, Arabidopsis; D029681:Arabidopsis Proteins; D008565:Membrane Proteins; D009842:Oligopeptides; D010940:Plant Proteins; D053830:Plant Viral Movement Proteins; D014764:Viral Proteins; C047981:arginyl-glycyl-aspartic acid