title: Galactan biosynthesis in Mycobacterium tuberculosis. Identification of a bifunctional UDP-galactofuranosyltransferase.
abstract: The cell wall of Mycobacterium tuberculosis and related genera is unique among prokaryotes, consisting of a covalently bound complex of mycolic acids, D-arabinan and D-galactan, which is linked to peptidoglycan via a special linkage unit consisting of Rhap-(1-->3)-GlcNAc-P. Information concerning the biosynthesis of this entire polymer is now emerging with the promise of new drug targets against tuberculosis. Accordingly, we have developed a galactosyltransferase assay that utilizes the disaccharide neoglycolipid acceptors beta-d-Galf-(1-->5)-beta-D-Galf-O-C(10:1) and beta-D-Galf-(1-->6)-beta-D-Galf-O-C(10:1), with UDP-Gal in conjunction with isolated membranes. Chemical analysis of the subsequent reaction products established that the enzymatically synthesized products contained both beta-D-Galf linkages ((1-->5) and (1-->6)) found within the mycobacterial cell, as well as in an alternating (1-->5) and (1-->6) fashion consistent with the established structure of the cell wall. Furthermore, through a detailed examination of the M. tuberculosis genome, we have shown that the gene product of Rv3808c, now termed glfT, is a novel UDP-galactofuranosyltransferase. This enzyme possesses dual functionality in performing both (1-->5) and (1-->6) galactofuranosyltransferase reactions with the above neoglycolipid acceptors, using membranes isolated from the heterologous host Escherichia coli expressing Rv3808c. Thus, at a biochemical and genetic level, the polymerization of the galactan region of the mycolyl-arabinogalactan complex has been defined, allowing the possibility of further studies toward substrate recognition and catalysis and assay development. Ultimately, this may also lead to a more rational approach to drug design to be explored in the context of mycobacterial infections.
authors: Department of Microbiology and Immunology, University of Newcastle upon Tyne, Newcastle upon Tyne, NE2 4HH, United Kingdom.
authors: L
authors: L
authors: Kremer
authors: L G
authors: LG
authors: Dover
authors: C
authors: C
authors: Morehouse
authors: P
authors: P
authors: Hitchin
authors: M
authors: M
authors: Everett
authors: H R
authors: HR
authors: Morris
authors: A
authors: A
authors: Dell
authors: P J
authors: PJ
authors: Brennan
authors: M R
authors: MR
authors: McNeil
authors: C
authors: C
authors: Flaherty
authors: K
authors: K
authors: Duncan
authors: G S
authors: GS
authors: Besra
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D005685:Galactans / Q000096:biosynthesis*; D005700:Galactosyltransferases / Q000378:metabolism*; D009169:Mycobacterium tuberculosis / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D001426:Bacterial Proteins; D005685:Galactans; D005700:Galactosyltransferases