FcgammaRIII b and FcgammaRIIa polymorphism may affect the production of specific NA1 autoantibody and clinical course of autoimmune neutropenia of infancy.
We studied 18 children with autoimmune neutropenia (AIN) to evaluate whether there was a possible relationship between the specificity of granulocyte autoantibodies (anti-NA1,2) and the phenotype of the NA system. Direct granulocyte immunofluorescence test (D-GIFT) was positive in all patients, and indirect granulocyte immunofluorescence test (I-GIFT) was positive in 17 of these 18 patients, respectively. Fourteen of 18 patients showed preferential binding to neutrophils from NA(1+2-) phenotyped donors. Immunoblotting with anti-FcgammaRIIImAb showed that IgG prepared from 7 of 12 patients precipitated both FcgammaRIIIb from NA1 and NA2 neutrophil lysate, whereas the other 5 precipitated only NA1. Patients' IgG did not react with purified FcgammaRIIa. FcgammaRIIIb genotype were NA(1+2-) in 15 of 18 patients and NA(1+2+) in the other 3. FcgammaRIIa type of all patients were (H+R-). These distributions were significantly different from those of healthy Japanese blood donors (n = 608). The genotype of FcgammaRIIIb and FcgammaRIIa may affect the production of neutrophil specific auto-antibodies in AIN of infancy and influence its clinical course.
Taniuchi
S
S
Department of Pediatrics, Kansai Medical University, Osaka, Japan. taniuchi@takaii.kmu.ac.jp
Masuda
M
M
Yamamoto
A
A
Hasui
M
M
Tsuji
S
S
Komiyama
Y
Y
Takahashi
H
H
Kobayashi
Y
Y
D017668:Age of Onset; D000918:Antibody Specificity / Q000276:immunology*; D015703:Antigens, CD / Q000235:genetics* / Q000276:immunology; D001323:Autoantibodies / Q000096:biosynthesis* / Q000097:blood / Q000276:immunology; D001324:Autoantigens / Q000276:immunology*; D001327:Autoimmune Diseases / Q000097:blood / Q000235:genetics / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D005260:Female; D019085:Fluorescent Antibody Technique, Direct; D019084:Fluorescent Antibody Technique, Indirect; D005838:Genotype; D006801:Humans; D007223:Infant; D007519:Isoantigens / Q000276:immunology*; D008297:Male; D009503:Neutropenia / Q000097:blood / Q000235:genetics / Q000276:immunology*; D011110:Polymorphism, Genetic; D017452:Receptors, IgG / Q000235:genetics* / Q000276:immunology
D015703:Antigens, CD; D001323:Autoantibodies; D001324:Autoantigens; C080568:Fc gamma receptor IIA; D007519:Isoantigens; D017452:Receptors, IgG; C067704:neutrophil-specific antigen NA1, human; C072753:neutrophil-specific antigen NA2