Capillary electrophoresis as a tool for optimization of multiplex PCR reactions.
Copying multiple regions of a DNA molecule is routinely performed today using the polymerase chain reaction (PCR) in a process commonly referred to as multiplex PCR. The development of a multiplex PCR reaction involves designing primer sets and examining various combinations of those primer sets and different reaction components and/or thermal cycling conditions. The process of optimizing a multiplex PCR reaction in order to obtain a well-balanced set of amplicons can be time-consuming and labor-intensive. The rapid separation and quantitation capabilities of capillary electrophoresis make it an efficient technique to help in the multiplex PCR optimization process.
Butler
J M
JM
National Institute of Standards and Technology, Biotechnology Division, Gaithersburg, MD 20899, USA. john.butler@nist.gov
Ruitberg
C M
CM
Vallone
P M
PM
D000483:Alleles; D001483:Base Sequence; D002874:Chromosome Mapping; D017931:DNA Primers; D019075:Electrophoresis, Capillary / Q000379:methods*; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D018807:Polymorphism, Single-Stranded Conformational; D020080:Tandem Repeat Sequences; D014998:Y Chromosome
D017931:DNA Primers