Correlations between matrix metalloproteinase-9 and adenosine deaminase isozymes in synovial fluid from patients with rheumatoid arthritis.
OBJECTIVE
To clarify the significance of increased adenosine deaminase (ADA) isozyme activities in synovial fluid (SF) from patients with rheumatoid arthritis (RA).


METHODS
ADA isozyme activities were measured using colorimetric assays. Concentrations of matrix metalloproteinase-9 (MMP-9) were measured by ELISA.


RESULTS
Levels of ADA isozyme activities in RA SF were significantly higher than those from patients with osteoarthritis and patients with traumatic injuries. Significant positive correlations between MMP-9 concentration and ADA activities were observed in RA SF (MMP-9 vs total ADA: r = 0.880; vs ADA1: r = 0.829; vs ADA2: r = 0.823; p < 0.001).


CONCLUSIONS
Elevated levels of ADA activities were found in SF from patients with RA. There were significant positive correlations between MMP-9 and ADA isozymes. These results may reflect the inflammatory condition of patients with RA.
Iwaki-Egawa
S
S
Department of Pathophysiology and Biochemistry, Hokkaido College of Pharmacy, Otaru, Japan.
WatanabeY
Matsuno
H
H
D000243:Adenosine Deaminase / Q000378:metabolism*; D001172:Arthritis, Rheumatoid / Q000378:metabolism*; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism*; D019272:Leukocyte Elastase / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D012044:Regression Analysis; D013582:Synovial Fluid / Q000201:enzymology*
D007527:Isoenzymes; D019272:Leukocyte Elastase; D020780:Matrix Metalloproteinase 9; D000243:Adenosine Deaminase