title: Expression of endothelial nitric oxide synthase in the postnatal developing porcine kidney.
abstract: The postnatal pattern of renal endothelial nitric oxide synthase (eNOS) is unknown. The purpose of this study was to characterize eNOS expression during maturation and compare this to neuronal NOS (nNOS). The experiments measured whole kidney eNOS mRNA expression by RT-PCR and protein content by Western blot, as well as cortical and medullary protein content in piglets at selected postnatal ages and in adult pigs. Whole kidney eNOS mRNA was compared with nNOS. Whole kidney eNOS expression decreased from the newborn to its lowest at 7 days, returning by 14 days to adult levels. This eNOS mRNA pattern contrasted with nNOS, which was highest at birth, and progressively decreased to its lowest level in the adult. At birth, cortical eNOS protein was greater than medullary, contrasting with the adult pattern of equivalent levels. In conclusion eNOS is developmentally regulated during early renal maturation and may critically participate in renal function during this period. The eNOS developmental pattern differs from nNOS, suggesting that these isoforms may have different regulatory factors and functional contributions in the postnatal kidney.
authors: Department of Pediatrics, Eastern Virginia Medical School, Norfolk, Virginia 23501, USA. solhaumj@evms.edu
authors: M J
authors: MJ
authors: Solhaug
authors: U
authors: U
authors: Kullaprawithaya
authors: X Q
authors: XQ
authors: Dong
authors: K W
authors: KW
authors: Dong
mesh_terms: D000375:Aging; D000818:Animals; D000831:Animals, Newborn; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D007668:Kidney / Q000201:enzymology / Q000254:growth & development*; D007672:Kidney Cortex / Q000201:enzymology / Q000254:growth & development; D007679:Kidney Medulla / Q000201:enzymology / Q000254:growth & development; D019001:Nitric Oxide Synthase / Q000235:genetics*; D052248:Nitric Oxide Synthase Type I; D052250:Nitric Oxide Synthase Type III; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine; D014158:Transcription, Genetic
chemical_list: D012333:RNA, Messenger; D019001:Nitric Oxide Synthase; D052248:Nitric Oxide Synthase Type I; D052250:Nitric Oxide Synthase Type III