Possible prevention by abieslactone of development of diethylnitrosamine-initiated GST-P positive foci in the rat liver.
Triterpenoid compounds, isolated from plants of Abies genus (Pinceae), are known to exert anti-tumor promotion activities in mouse skin carcinogenesis. In the present study, we investigated whether AVB-1 and acid and acid methyl ester derivatives have inhibitory effects on rat hepatocarcinogenesis by using a liver medium-term bioassay for carcinogens (Ito's test), immunohistochemically assessing the numbers and areas per cm(2) of preneoplastic lesions, glutathione S-transferase placental form (GST-P)-positive foci. In experiment 1, 6-week-old male Fisher 344 rats were given a single intraperitoneal injection of diethylnitrosamine (200 mg/kg b.w.) and subjected to two-thirds partial hepatectomy at week 3. From weeks 2 to 8, the compounds were given three times a week at a dose of 1 mg/kg b.w. by i.g. gavage and found to significantly decrease the number of GST-P-positive foci in the liver. In experiment 2, AVB-1 was given three times a week at doses of 3, 1, or 0.3 mg/kg b.w. by i.g. gavage from weeks 2 to 8. All doses of AVB-1 significantly decreased the numbers of GST-P-positive foci. Thus, our results suggest that AVB-1 is a chemopreventive agent for rat hepatocarcinogenesis.
Iwai
S
S
Department of Pathology, Osaka City University Medical School, Osaka, Japan.
Wei
M
M
Morimura
K
K
Wanibuchi
H
H
Tanaka
R
R
Matsunaga
S
S
Yoshitake
A
A
Seki
S
S
Fukushima
S
S
D000477:Alkylating Agents / Q000633:toxicity*; D000818:Animals; D016588:Anticarcinogenic Agents / Q000494:pharmacology* / Q000627:therapeutic use; D001835:Body Weight / Q000187:drug effects; D018890:Chemoprevention; D004052:Diethylnitrosamine / Q000633:toxicity*; D005982:Glutathione Transferase / Q000378:metabolism*; D007783:Lactones / Q000494:pharmacology* / Q000627:therapeutic use; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced / Q000201:enzymology / Q000517:prevention & control*; D008297:Male; D014411:Neoplastic Stem Cells; D010920:Placenta / Q000201:enzymology*; D051381:Rats; D011916:Rats, Inbred F344; D014315:Triterpenes / Q000494:pharmacology* / Q000627:therapeutic use
D000477:Alkylating Agents; D016588:Anticarcinogenic Agents; D007783:Lactones; D014315:Triterpenes; C066058:abieslactone; D004052:Diethylnitrosamine; D005982:Glutathione Transferase