title: Histopathologic analysis of endovascular stent grafts from patients with aortic aneurysms: Does healing occur?
abstract: BACKGROUND
  Research with animal models has demonstrated tissue healing of endovascular grafts in both native arterial segments and in experimentally created arterial aneurysms. Fundamental to the successful clinical use of endovascular grafts for the treatment of aneurysmal disease is the creation of a permanent hemostatic seal between the graft ends and the arterial wall. Characteristics of this healing process in patients with aneurysmal disease have not been fully studied. In this study, we analyzed the macroscopic and histopathologic changes of the arterial wall after endovascular repair of aortic aneurysms.
  
  
  METHODS
  Over a 7-year period, 313 patients were treated with endovascular grafts to exclude arterial aneurysms of the thoracic and abdominal aorta. Of these patients, 11 had their endovascular grafts recovered for analysis. Five graft specimens were recovered during subsequent open aortic surgery. Six grafts were recovered at autopsy after the death of the patient of causes unrelated to the patient's endovascular graft. All specimens were fixed in formalin. Histologic analysis included light microscopy with hematoxylin and eosin and trichrome stains. Well-preserved specimens were selected after light microscopic examination and postfixed in 3% buffered glutaraldehyde for electron microscopy. The aortas from autopsy specimens were removed en bloc and fixed in formalin; representative regions of each graft were sectioned for analysis. Adherence of the graft to the vessel wall was categorized as densely adherent or easily separated after graft explantation. Traction applied to the graft-aortic anastomosis was equal to traction generated by suspending a standardized 2-kg weight. Infrarenal graft specimens were obtained with supraceliac aortic clamping, longitudinal aortotomy, and graft sampling before endograft revision.
  
  
  RESULTS
  In eight patients, endograft fixation was found to be firmly adherent to the arterial wall. A translucent film of fibrinous material was consistently seen across the entire luminal surface of the endograft. Light and electron microscopy failed to demonstrate an endothelial layer or organized pseudointima at the graft-artery interface.
  
  
  CONCLUSIONS
  Despite suggestive experimental data regarding endograft healing in animals, minimal graft incorporation was apparent in the stent grafts recovered in this study. A greater emphasis on the construction and mechanism of fixation of endograft attachment systems will be important for long-term device function.
authors: Division of Vascular Surgery, Department of Surgery, Mount Sinai School of Medicine, New York, NY, USA.
authors: C
authors: C
authors: McArthur
authors: V
authors: V
authors: Teodorescu
authors: L
authors: L
authors: Eisen
authors: N
authors: N
authors: Morrissey
authors: P
authors: P
authors: Faries
authors: L
authors: L
authors: Hollier
authors: M L
authors: ML
authors: Marin
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001011:Aorta / Q000473:pathology*; D001014:Aortic Aneurysm / Q000473:pathology* / Q000601:surgery; D019917:Blood Vessel Prosthesis Implantation; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D015607:Stents