Enzyme-linked immunosorbent assay for detection and quantification of Cryptococcus neoformans antigen.
An enzyme-linked immunosorbent assay was standardized for the detection of cryptococcal antigen in serum and cerebrospinal fluid. The system was evaluated in clinical samples from patients infected by human immunodeficiency virus with and without previous cryptococcosis diagnosis. The evaluated system is highly sensitive and specific, and when it was compared with latex agglutination there were not significant differences. A standard curve with purified Cryptococcus neoformans antigen was settled down for the antigen quantification in positive samples.
Illnait
M T
MT
Laboratorio de Micología, Instituto Pedro Kourí, Havana, Cuba.
Vilaseca
J C
JC
Fernández
C M
CM
Martínez
G F
GF
D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology*; D000946:Antigens, Fungal / Q000032:analysis* / Q000302:isolation & purification; D003453:Cryptococcosis / Q000175:diagnosis / Q000276:immunology; D003455:Cryptococcus neoformans / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007841:Latex Fixation Tests; D011134:Polysaccharides / Q000032:analysis* / Q000276:immunology
D000946:Antigens, Fungal; D011134:Polysaccharides; C027478:glucuronoxylomannan