title: SDE3 encodes an RNA helicase required for post-transcriptional gene silencing in Arabidopsis.
abstract: Post-transcriptional gene silencing (PTGS) provides protection in plants against virus infection and can suppress expression of transgenes. Arabidopsis plants carrying mutations at the SDE3 locus are defective in PTGS mediated by a green fluorescent protein transgene. However, PTGS mediated by tobacco rattle virus (TRV) was not affected by sde3. From these results we conclude that SDE3, like the previously described RNA polymerase encoded by SDE1, acts at a stage in the mechanism that is circumvented when PTGS is mediated by TRV. The product of SDE3 is similar to RNA helicase-like proteins including GB110 in mouse and other proteins in Drosophila and humans. These proteins are similar to, but clearly distinct from Upf1p and SMG-2, which are required for nonsense-mediated mRNA decay in yeast and Caenorhabditis elegans and, in the case of SMG-2, for PTGS.
authors: The Sainsbury Laboratory, John Innes Centre, Colney Lane, Norwich NR4 7UH, UK.
authors: T
authors: T
authors: Dalmay
authors: R
authors: R
authors: Horsefield
authors: T H
authors: TH
authors: Braunstein
authors: D C
authors: DC
authors: Baulcombe
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics* / Q000821:virology; D029681:Arabidopsis Proteins; D002874:Chromosome Mapping; D017799:Cucumovirus / Q000235:genetics / Q000472:pathogenicity; D020868:Gene Silencing; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D010942:Plant Viruses / Q000235:genetics / Q000472:pathogenicity; D030821:Plants, Genetically Modified; D020365:RNA Helicases / Q000235:genetics* / Q000378:metabolism; D012323:RNA Processing, Post-Transcriptional; D012328:RNA Viruses / Q000235:genetics / Q000472:pathogenicity; D012330:RNA, Double-Stranded / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D029681:Arabidopsis Proteins; D010940:Plant Proteins; D012330:RNA, Double-Stranded; C426019:SDE3 protein, Arabidopsis; D020365:RNA Helicases