Preparation of oligodeoxynucleotides containing 5-(N-methylpiperazinyl) and 5-benzyloxymethyl uracils.
Deprotected compounds 1 and 9 were allowed to react with 4,4'-dimethoxytrityl chloride in pyridine to give 5'-O-DMT nucleosides 2 and 10. The 3'-phosphoramidites 4 and 11 were incorporated into oligodeoxynucleosides (ODNs). The hybridization properties of the modified ODNs with their complementary DNA strands were studied. Interesting results were obtained when 11 was inserted as a bulged nucleoside into TWAs, duplexes, and triplexes.
Abdel Megied
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AE
Department of Chemistry, Faculty of Science, Menoufia University, Shebien El-Koam, Egypt. abdel_megied@yahoo.com
Ali
O M
OM
Kofoed
T
T
Pedersen
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EB
D001593:Benzyl Compounds / Q000737:chemistry*; D018076:DNA, Complementary / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000737:chemistry*; D010879:Piperazines / Q000737:chemistry*; D014498:Uracil / Q000031:analogs & derivatives* / Q000737:chemistry*
D001593:Benzyl Compounds; D018076:DNA, Complementary; D009838:Oligodeoxyribonucleotides; D010879:Piperazines; D014498:Uracil