Immunolocalization of p53, glutathione S-tranferase pi and CD57 antigens in oral leukoplakia.
OBJECTIVE
The purpose of the present study was to evaluate the relationship between histological epithelial dysplasia, the immunolocalization of p53 and glutathione S-transferase pi on the immunolocalization of the CD57 antigen.


METHODS
Seventy biopsies were included in the study and the streptavidin-biotin-peroxidase method was used to detect the antigens.


RESULTS
The results demonstrated a significant relationship between p53, GST-pi positive staining with moderate/severe epithelial dysplasia. There was no relationship between p53 and GST-pi. The mean number of CD57+ lymphoid cells was higher in the lesions with increased epithelial dysplasia and positive for GST-pi. No difference was found regarding CD57 immunolocalization in leukoplakias positive and negative for p53.


CONCLUSIONS
As the presence of CD57+ lymphoid cells are indicative of immunosuppression, our study suggests that the severity of epithelial dysplasia and positive immunolabelling for GST-pi are associated with local immune response alterations in oral leukoplakias. Our data also give support to the idea that GST-pi and p53 are not time-point related during oral cancer development.
De Paula
A M
AM
Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais, Rua Conde de Linhares, Belo Horizonte, Brazil.
Gomez
R S
RS
D014408:Biomarkers, Tumor / Q000032:analysis*; D018959:CD57 Antigens / Q000032:analysis* / Q000276:immunology; D004848:Epithelium / Q000737:chemistry / Q000473:pathology; D051549:Glutathione S-Transferase pi; D005982:Glutathione Transferase / Q000032:analysis* / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques; D007527:Isoenzymes / Q000032:analysis* / Q000276:immunology; D007972:Leukoplakia, Oral / Q000737:chemistry / Q000175:diagnosis* / Q000276:immunology; D016246:Lymphocytes, Tumor-Infiltrating / Q000737:chemistry; D016159:Tumor Suppressor Protein p53 / Q000032:analysis* / Q000276:immunology
D014408:Biomarkers, Tumor; D018959:CD57 Antigens; D007527:Isoenzymes; D016159:Tumor Suppressor Protein p53; C496556:GSTP1 protein, human; D051549:Glutathione S-Transferase pi; D005982:Glutathione Transferase