Rat coagulating gland secretion contains a kinesin heavy chain-like protein acting as a type IV transglutaminase substrate.
By a proteomic approach, we demonstrated in rat coagulating gland secretion the presence of a 120 kDa protein which shares at least 80% identity at the amino acid level with the most closely related kinesin heavy chain codified by the kinesin superfamily protein Kif5c gene. In addition, we identified 30 and 66 kDa proteolytic fragments of such a kinesin heavy chain-like protein, corresponding to the 73-299 N-terminal and 300-860 C-terminal regions, respectively. Finally, we demonstrated the occurrence in coagulating gland secretion of a 200 kDa protein probably derived by cross-linking reaction of the kinesin heavy chain-like protein with type IV transglutaminase. In fact, kinesin heavy chain-like protein and its 66 kDa proteolytic fragment were also found to act as effective acyl donor substrates for the enzyme in vitro.
Esposito
C
C
Department of Chemistry, University of Salerno, 84081 Baronissi (SA), Italy. esposito@chem.unisa.it
Mariniello
L
L
Cozzolino
A
A
Amoresano
A
A
Orrù
S
S
Porta
R
R
D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D002250:Carbon Radioisotopes / Q000378:metabolism; D016547:Kinesins / Q000302:isolation & purification / Q000378:metabolism*; D008297:Male; D051379:Mice; D020409:Molecular Motor Proteins / Q000302:isolation & purification / Q000378:metabolism; D008969:Molecular Sequence Data; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D029589:Seminal Plasma Proteins; D012669:Seminal Vesicles / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013095:Spermidine / Q000378:metabolism; D013379:Substrate Specificity; D011503:Transglutaminases / Q000378:metabolism*
D002250:Carbon Radioisotopes; C419428:Kifc3 protein, mouse; D020409:Molecular Motor Proteins; D011506:Proteins; D029589:Seminal Plasma Proteins; D011503:Transglutaminases; D016547:Kinesins; D013095:Spermidine