Mass spectrometric determination of O-glycosylation sites using beta-elimination and partial acid hydrolysis.
The present study demonstrates that treating O-glycosylated peptides with methylamine vapor followed by partial acid hydrolysis is an effective means for locating O-glycosylation site(s). The reaction with methylamine transforms the glycosylated Ser and Thr residues into stable methylamine derivatives with a mass increment of +13 Da relative to nonglycosylated Ser and Thr residues. Peptide sequencing based on partial acid hydrolysis followed by mass spectrometric analysis or in favorable cases by CID-MS/MS enables the determination of the formerly O-glycosylated sites.
Mirgorodskaya
E
E
Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense University.
Hassan
H
H
Clausen
H
H
Roepstorff
P
P
D000143:Acids; D006031:Glycosylation; D006868:Hydrolysis; D013058:Mass Spectrometry; D010455:Peptides / Q000737:chemistry*
D000143:Acids; D010455:Peptides