title: Role of mast cells in chronic stress induced colonic epithelial barrier dysfunction in the rat.
abstract: OBJECTIVE
  Stress may be an important factor in exacerbating inflammatory bowel disease but the underlying mechanism is unclear. Defective epithelial barrier function may allow uptake of luminal antigens that stimulate an immune/inflammatory response. Here, we examined the effect of chronic stress on colonic permeability and the participation of mast cells in this response.
  
  
  METHODS
  Mast cell deficient Ws/Ws rats and +/+ littermate controls were submitted to water avoidance stress or sham stress (one hour/day) for five days. Colonic epithelial permeability to a model macromolecular antigen, horseradish peroxidase, was measured in Ussing chambers. Epithelial and mast cell morphology was studied by light and electron microscopy.
  
  
  RESULTS
  Chronic stress significantly increased macromolecular flux and caused epithelial mitochondrial swelling in +/+ rats, but not in Ws/Ws rats, compared with non-stressed controls. Stress increased the number of mucosal mast cells and the proportion of cells showing signs of activation in +/+ rats. No mast cells or ultrastructural abnormalities of the epithelium were present in Ws/Ws rats. Increased permeability in +/+ rats persisted for 72 hours after stress cessation.
  
  
  CONCLUSIONS
  Chronic stress causes an epithelial barrier defect and epithelial mitochondrial damage, in parallel with mucosal mast cell hyperplasia and activation. The study provides further support for an important role for mast cells in stress induced colonic mucosal pathophysiology.
authors: Intestinal Disease Research Program, Department of Pathology and Molecular Medicine, Faculty of Health Sciences, McMaster University, Hamilton, Ontario, L8N 3Z5, Canada.
authors: J
authors: J
authors: Santos
authors: P C
authors: PC
authors: Yang
authors: J D
authors: JD
authors: Söderholm
authors: M
authors: M
authors: Benjamin
authors: M H
authors: MH
authors: Perdue
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D002463:Cell Membrane Permeability / Q000502:physiology*; D004553:Electric Conductivity; D005772:Gastrointestinal Transit / Q000502:physiology; D006735:Horseradish Peroxidase / Q000378:metabolism; D007413:Intestinal Mucosa / Q000503:physiopathology* / Q000648:ultrastructure; D008407:Mast Cells / Q000502:physiology* / Q000648:ultrastructure; D008854:Microscopy, Electron; D008928:Mitochondria / Q000473:pathology; D051381:Rats; D011922:Rats, Mutant Strains; D018709:Statistics, Nonparametric; D013315:Stress, Psychological / Q000503:physiopathology*
chemical_list: D006735:Horseradish Peroxidase