Muscle prelamination with urothelial cell cultures via fibrin glue in rats.
The purpose of the study was to transplant autologous cultured urothelial cells onto a muscle via fibrin glue as a delivery vehicle to create a vascularized, living matrix lined with urothelium that could subsequently be used for urinary reconstruction. Bladder tissue specimens from male Wistar rats (n = 32; 350--500 g) were harvested for urothelial tissue culture. After 8--10 days when the primary cultures became confluent, the cultured urothelial cells were injected underneath the rectus sheath onto the rectus muscle. As delivery vehicle we compared standard culture media and fibrin glue. At 1- and 4-week intervals following urothelial cell grafting, sections of the muscle were analyzed for urothelial graft take using Hematoxylin & Eosin and immunohistochemical staining. The histology demonstrated viable, multilayered clusters of urothelium cells on the muscle surface only in the group using the fibrin glue delivery vehicle. We conclude that a muscle can be successfully prelaminated with autologously cultured urothelial cells via fibrin glue and has therefore potential for urinary reconstructions.
Wechselberger
G
G
Department of Plastic and Reconstructive Surgery, Leopold-Franzens University Innsbruck, Austria. Gottfried.wechselberger@uibk.ac.at
Bauer
T
T
Meirer
R
R
Piza-Katzer
H
H
Lille
S
S
Russell
R C
RC
Schoeller
T
T
D000818:Animals; D017690:Cell Transplantation; D002478:Cells, Cultured; D003470:Culture Media; D015718:Fibrin Tissue Adhesive; D019085:Fluorescent Antibody Technique, Direct; D007150:Immunohistochemistry; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D017568:Rectus Abdominis / Q000601:surgery*; D019459:Urothelium / Q000166:cytology* / Q000637:transplantation*
D003470:Culture Media; D015718:Fibrin Tissue Adhesive