Induction and characterization of hypoxanthine-phosphoribosyltransferase (Hprt-) deficient cell lines of Akodon cursor (Rodentia, Sigmodontinae).
Hypoxanthine-phosphoribosyltransferase negative (Hprt-) cell lines derived from an Akodon cursor liposarcoma were obtained by induced mutagenesis. All but one Hprt- cell line lacked Hprt mRNA transcripts while one (AKO 3) coded for a truncated protein. Cell fusion and karyotypic analyses showed that one cell line (AKO 1-15) could be successfully used for constructing hybrid panels and allow for a clear identification of human chromosomes in hybrid cells.
Bonvicino
C R
CR
Divisão de Genética, Instituto Nacional de Câncer, Rio de Janeiro, Brazil.
Moreira
M A
MA
Arcuri
R A
RA
Seuánez
H N
HN
D000782:Aneuploidy; D000818:Animals; D002459:Cell Fusion; D002875:Chromosomes / Q000235:genetics*; D006801:Humans; D006822:Hybrid Cells / Q000166:cytology* / Q000201:enzymology* / Q000378:metabolism; D007041:Hypoxanthine Phosphoribosyltransferase / Q000737:chemistry / Q000172:deficiency* / Q000235:genetics; D007621:Karyotyping; D008080:Liposarcoma / Q000201:enzymology / Q000235:genetics / Q000473:pathology; D008214:Lymphocytes / Q000166:cytology / Q000378:metabolism; D009115:Muridae / Q000235:genetics*; D016296:Mutagenesis / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017384:Sequence Deletion / Q000235:genetics; D014407:Tumor Cells, Cultured
D012333:RNA, Messenger; D007041:Hypoxanthine Phosphoribosyltransferase