Outer sheath associated proteins of the oral spirochete Treponema maltophilum.
We recently cloned the major outer membrane protein of Treponema maltophilum [Heuner, K., Choi, B.K., Schade, R., Moter, A., Otto, A., Göbel, U.B., J. Bacteriol. 181, 1025-1029]. Here we report the localization of the major sheath protein (Msp)A protein in T. maltophilum by immunogold electron microscopy and its expression. Northern blot analysis revealed that mspA is expressed constitutively as a monocistronic unit. The transcription initiation site of the mspA gene was identified by primer extension analysis. A further screening of a genomic library of T. maltophilum with an anti-outer membrane fraction antibody was done. We were able to clone DNA regions of T. maltophilum encoding putative sugar transport operons and putative outer membrane proteins of this oral treponeme which has a high prevalence in periodontal lesions.
Heuner
K
K
Institut für Mikrobiologie und Hygiene, Universitätsklinikum Charité, Humboldt-Universität zu Berlin, Germany. klaus.heuner@mail-wuerburg.de
Meltzer
U
U
Choi
B K
BK
Göbel
U B
UB
D001425:Bacterial Outer Membrane Proteins / Q000032:analysis* / Q000096:biosynthesis / Q000235:genetics; D001426:Bacterial Proteins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D015152:Blotting, Northern; D015153:Blotting, Western; D050260:Carbohydrate Metabolism; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors; D015698:Genomic Library; D008565:Membrane Proteins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D016253:Microscopy, Immunoelectron; D008969:Molecular Sequence Data; D009876:Operon; D012329:RNA, Bacterial / Q000032:analysis; D011994:Recombinant Proteins / Q000096:biosynthesis; D014170:Transformation, Genetic; D014208:Treponema / Q000737:chemistry* / Q000235:genetics
D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D008565:Membrane Proteins; D012329:RNA, Bacterial; D011994:Recombinant Proteins