title: Characterization and localization of expression of an erythropoietin-induced gene, ERIC-1/TACC3, identified in erythroid precursor cells.
abstract: Gene expression profiles during erythropoietin (Epo)-induced differentiation of erythroid progenitor cells derived from the Friend virus anaemia (FVA) and phenylhydrazine (PHZ) murine models have been examined using differential display polymerase chain reaction (PCR). Ten cDNA fragments upregulated by Epo were isolated. The ribonuclease protection assay confirmed differential expression between Epo-stimulated and Epo-deprived cells for one of these, provisionally named ERIC-1. Sequencing of the full-length cDNA predicted a protein of 558 amino acids, 17 amino acids longer than mTACC3, the third member of a novel family of proteins that contain a coiled-coil domain. The human homologue, cloned using rapid amplification of cDNA ends (RACE)-PCR, encodes a larger protein of 838 amino acids that is identical to hTACC3. In addition to erythroid precursor cells, ERIC-1/TACC3 is expressed at high levels in the testes, at moderate levels in the thymus and peripheral leucocytes, and at lower levels in the spleen and intestinal tissue. Immunohistochemical analysis using an antibody to a GST fusion product of the C-terminus of hERIC-1/TACC3 revealed that it is localized to Sertoli cells in the human testes. Confocal microscopy demonstrated hERIC-1/TACC3 protein concentrated in the perinuclear vesicles of dermal microvascular endothelial cells. Although ERIC-1/TACC3 is expressed in a wide range of tissues, its upregulation by Epo in erythroid progenitors implies that it has a role in terminal erythropoiesis.
authors: Department of Nephrology, Belfast City Hospital, The Queen's University of Belfast, Tower Block, Lisburn Road, Belfast BT9 7AB, Northern Ireland, UK.
authors: P J
authors: PJ
authors: McKeveney
authors: V M
authors: VM
authors: Hodges
authors: R N
authors: RN
authors: Mullan
authors: P
authors: P
authors: Maxwell
authors: D
authors: D
authors: Simpson
authors: A
authors: A
authors: Thompson
authors: P C
authors: PC
authors: Winter
authors: T R
authors: TR
authors: Lappin
authors: A P
authors: AP
authors: Maxwell
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D015672:Erythroid Precursor Cells / Q000502:physiology*; D004920:Erythropoiesis / Q000235:genetics*; D004921:Erythropoietin / Q000502:physiology*; D005622:Friend murine leukemia virus; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D004915:Leukemia, Erythroblastic, Acute / Q000235:genetics*; D008297:Male; D051379:Mice; D008869:Microtubule-Associated Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D010659:Phenylhydrazines; D016133:Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012708:Sertoli Cells / Q000502:physiology; D014407:Tumor Cells, Cultured
chemical_list: D008869:Microtubule-Associated Proteins; D010659:Phenylhydrazines; C120551:TACC3 protein, human; C030299:phenylhydrazine; D004921:Erythropoietin