Pattern of cytokine and adhesion molecule mRNA in hapten-induced relapsing colon inflammation in the rat.
We examined the mRNA expression of cytokines, chemokines, integrins, and selectins in colon lesions of rat colitis with a semi-quantitative RT-PCR assay. Rat colitis was induced by trinitrobenzene sulfonic acid (TNBS) in 50% ethanol. Within 24 h, an acute inflammation occurred with hyperemia, edema, necrosis and an intense infiltration of granulocytes in the mucosa. The lesion proceeded into a T-lymphocyte/monocyte-driven chronic inflammation for two weeks and healed in 6 weeks. An acute inflammation recurred at the same site when the recovered animals were systemically injected with TNBS. We isolated RNA from colon tissue at 24 h, 1, 2, 4, 6 weeks after TNBS treatment and from the relapsed animals. The mRNA for cytokines IL-1beta, IL-6, IL-10 and the chemokines CINC, MIP-1alpha, MCP-1 were significantly (P < 0.05) elevated and persisted for 2 weeks, decreased in 6 weeks and increased again during relapse. IFN-gamma mRNA stayed at control levels initially, but increased dramatically in the second weeks of chronic inflammation as well as in relapse. The mRNA levels of adhesion molecules, ICAM-1, VCAM-1, the mucosal homing integrin beta7 as well as P- and E-selectin were greatly enhanced between 1 and 3 weeks. The data showed that the chronically inflamed tissue expresses a time-dependent changing pattern of TH1 cytokines and adhesion molecules that maintain the infiltration and activation of inflammatory cells and tissue injury.
Sun
F F
FF
Department of Cell Biology, School of Osteopathic Medicine, UMDNJ, Stratford, New Jersey 08084, USA.
Lai
P S
PS
Yue
G
G
Yin
K
K
Nagele
R G
RG
Tong
D M
DM
Krzesicki
R F
RF
Chin
J E
JE
Wong
P Y
PY
D000818:Animals; D015815:Cell Adhesion Molecules / Q000096:biosynthesis / Q000235:genetics*; D002908:Chronic Disease; D003092:Colitis / Q000139:chemically induced / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D016207:Cytokines / Q000096:biosynthesis / Q000235:genetics*; D004195:Disease Models, Animal; D018450:Disease Progression; D019040:E-Selectin / Q000096:biosynthesis / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects*; D006098:Granulocytes / Q000473:pathology; D006241:Haptens / Q000633:toxicity*; D015212:Inflammatory Bowel Diseases / Q000235:genetics / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D039641:Integrin beta Chains; D016023:Integrins / Q000096:biosynthesis / Q000235:genetics; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis / Q000235:genetics; D007371:Interferon-gamma / Q000096:biosynthesis / Q000235:genetics; D007413:Intestinal Mucosa / Q000378:metabolism / Q000473:pathology; D009000:Monocytes / Q000473:pathology; D019007:P-Selectin / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012008:Recurrence; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013601:T-Lymphocytes / Q000473:pathology; D014158:Transcription, Genetic / Q000187:drug effects; D014302:Trinitrobenzenesulfonic Acid / Q000633:toxicity*; D019010:Vascular Cell Adhesion Molecule-1 / Q000096:biosynthesis / Q000235:genetics
D015815:Cell Adhesion Molecules; D016207:Cytokines; D019040:E-Selectin; D006241:Haptens; D039641:Integrin beta Chains; D016023:Integrins; D019007:P-Selectin; D012333:RNA, Messenger; D019010:Vascular Cell Adhesion Molecule-1; C091368:integrin beta7; D018799:Intercellular Adhesion Molecule-1; D007371:Interferon-gamma; D014302:Trinitrobenzenesulfonic Acid