Luteolin inhibits the growth and arylamine N-acetyl-transferase activity in Neisseria gonorrhoeae.
Growth inhibition and arylamine N-acetyltransferase (NAT) activity in Neisseria gonorrhoeae were inhibited by luteolin, a drug which originated from herbs. The growth inhibition was based on changes in optical density (OD) using a spectrophotometer, and arylamine NAT activity with 2-aminofluorene (2-AF) was determined using high pressure liquid chromatography. The inhibition of growth in N. gonorrhoeae demonstrated that luteolin elicited a dose-dependent growth inhibition in the N. gonorrhoeae cultures. Suspensions of N. gonorrhoeae with or without specific concentrations of luteolin cotreatment showed different percentages of 2-AF acetylation. The data indicated that there was reduced NAT activity associated with increased levels of luteolin in N. gonorrhoeae suspensions. Time-course experiments showed that NAT activity measured from intact N. gonorrhoeae cells was inhibited by luteolin for at least 4 h. Using standard steady-state kinetic analysis, it was demonstrated that luteolin was a possible uncompetitive inhibitor to NAT activity in N. gonorrhoeae. This report is the first to show that luteolin can inhibit N. gonorrhoeae NAT activity.
Tsou
M F
MF
Department of Clinical Laboratory, China Medical College Hospital, Taichung, Taiwan, Republic of China.
Chen
G W
GW
Hung
C F
CF
Yeh
F T
FT
Chang
H L
HL
Lu
H F
HF
Chung
J G
JG
D001191:Arylamine N-Acetyltransferase / Q000037:antagonists & inhibitors*; D004305:Dose-Response Relationship, Drug; D005419:Flavonoids / Q000494:pharmacology*; D006069:Gonorrhea / Q000382:microbiology; D006801:Humans; D007700:Kinetics; D047311:Luteolin; D009344:Neisseria gonorrhoeae / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development; D013997:Time Factors
D005419:Flavonoids; D001191:Arylamine N-Acetyltransferase; D047311:Luteolin