title: Flow cytometry cannot assess surface binding of perforin to target cells.
authors: S S
authors: SS
authors: Metkar
authors: M
authors: M
authors: Aguilar-Santelises
authors: B
authors: B
authors: Wang
authors: C J
authors: CJ
authors: Froelich
mesh_terms: D002462:Cell Membrane / Q000737:chemistry; D002463:Cell Membrane Permeability / Q000187:drug effects; D017077:Culture Media, Conditioned / Q000494:pharmacology; D004351:Drug Resistance; D005434:Flow Cytometry; D006801:Humans; D007424:Intracellular Fluid / Q000737:chemistry; D019169:Jurkat Cells / Q000187:drug effects / Q000378:metabolism; D020014:K562 Cells / Q000187:drug effects; D007694:Killer Cells, Natural / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000032:analysis / Q000378:metabolism* / Q000494:pharmacology; D008954:Models, Biological; D054353:Perforin; D052899:Pore Forming Cytotoxic Proteins; D011485:Protein Binding; D012107:Research Design; D013048:Specimen Handling; D013194:Staining and Labeling; D014407:Tumor Cells, Cultured / Q000187:drug effects
chemical_list: D017077:Culture Media, Conditioned; D008562:Membrane Glycoproteins; D052899:Pore Forming Cytotoxic Proteins; D054353:Perforin