Evaluation of total PSA assay on vitros ECi and correlation with Kryptor-PSA assay.
BACKGROUND
An increasing number of multiparametric immuno-analysers for PSA assays are available. As different immuno-assays may vary in their analytical quality and their accuracy for the follow-up of patients, expertise is necessary for each new assay.


METHODS
The PSA assay on the Vitros-ECi analyser has been evaluated and compared with the PSA assay from the Kryptor analyser.


RESULTS
Variation coefficients were 0.91 to 1.98% for within-run assays, and 4.2% to 5.4% for interassay (PSA levels = 0.8 microgram/L to 33.6 micrograms/L). Dilution tests showed 93 to 136% recovery until 70 micrograms/L PSA. Functional sensitivity was estimated at 0.03 microgram/L. Equimolarity of the test was confirmed. Correlation of PSA levels measured with Vitros-ECi and Kryptor analysers displayed a correlation coefficient r2 of 0.9716. The half-lives and doubling times of PSA were similar using both methods.


CONCLUSIONS
Vitros-ECi PSA assay meets the major criteria for the management of prostate cancer patients.
Cassinat
B
B
Nuclear Medicine Department, Hôpital Saint Louis, 1 Av Claude Vellefaux, 75010 Paris, France.
Wacquet
M
M
Toubert
M E
ME
Rain
J D
JD
Schlageter
M H
MH
D000230:Adenocarcinoma / Q000097:blood*; D000911:Antibodies, Monoclonal / Q000276:immunology; D014408:Biomarkers, Tumor / Q000097:blood*; D019084:Fluorescent Antibody Technique, Indirect / Q000295:instrumentation; D005456:Fluorescent Dyes / Q000032:analysis*; D005500:Follow-Up Studies; D006207:Half-Life; D006801:Humans; D007124:Immunoenzyme Techniques / Q000295:instrumentation; D008163:Luminescent Measurements; D008297:Male; D009363:Neoplasm Proteins / Q000097:blood*; D009942:Organometallic Compounds / Q000032:analysis*; D017430:Prostate-Specific Antigen / Q000097:blood*; D011471:Prostatic Neoplasms / Q000097:blood*; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D005456:Fluorescent Dyes; D009363:Neoplasm Proteins; D009942:Organometallic Compounds; C070734:europium(III) trisbipyridine cryptate; D017430:Prostate-Specific Antigen