Principles of DNA separation with capillary electrophoresis.
During the last decade, capillary electrophoresis (CE) of DNA has undergone rapid development. This improvement was especially important for DNA sequencing, where CE has now become a standard method facilitating to decipher several genomes within a very short time. Here, we give a review of the fundamentals of DNA separation in CE and the major factors influencing the performance.
Heller
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Max-Planck-Institut für molekulare Genetik, Berlin-Dahlem, Germany. heller@biochip.com
D004247:DNA / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D019075:Electrophoresis, Capillary / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D005782:Gels; D008956:Models, Chemical; D008970:Molecular Weight; D009994:Osmolar Concentration; D011108:Polymers; D013499:Surface Properties; D014783:Viscosity
D005782:Gels; D011108:Polymers; D004247:DNA