A simple and rapid method for intra- and interspecific transformation of Bacillus subtilis on solid media by DNA in protoplast lysates.
A simple method for intra- and interspecific transformation of Bacillus subtilis on solid media has been devised with DNA in protoplast lysates, 0.8% agar, glutamate, and yeast extract. The transformation frequency is 2.3 x 10(3) transformants per microg DNA, 10-20 times higher than that for conventional transformation on solid media. The method can be applicable to transformation in microtiter plates.
Akamatsu
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T
Department of Applied Microbial Technology, Sojo University, Kumamoto, Japan. akamatsu@bio.sojo-u.ac.jp
Taguchi
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H
D001412:Bacillus subtilis / Q000235:genetics*; D003470:Culture Media; D004269:DNA, Bacterial / Q000235:genetics; D004274:DNA, Recombinant / Q000235:genetics; D005821:Genetic Techniques; D011523:Protoplasts; D014170:Transformation, Genetic
D003470:Culture Media; D004269:DNA, Bacterial; D004274:DNA, Recombinant