title: Molecular characterization of the genome of Maize rayado fino virus, the type member of the genus Marafivirus.
abstract: The complete nucleotide sequence of the single-stranded RNA genome of Maize rayado fino virus (MRFV), the type member of the genus Marafivirus, is 6305 nucleotides (nts) in length and contains two putative open reading frames (ORFs). The largest ORF (nt 97-6180) encodes a polyprotein of 224 kDa with sequence similarities at its N-terminus to the replication-associated proteins of other viruses with positive-strand RNA genomes and to the papainlike protease domain found in tymoviruses. The C-terminus of the 224-kDa ORF also encodes the MRFV capsid protein. A smaller, overlapping ORF (nt 302-1561) encodes a putative protein of 43 kDa with unknown function but with limited sequence similarities to putative movement proteins of tymoviruses. The nucleotide sequence and proposed genome expression strategy of MRFV is most closely related to that of oat blue dwarf virus (OBDV). Unlike OBDV, MRFV RNA does not appear to contain a poly(A) tail, and it encodes a putative second overlapping open reading frame.
authors: Molecular Plant Pathology Laboratory, United States Department of Agriculture, Beltsville, Maryland 20705, USA. hammondr@ba.ars.usda.gov
authors: R W
authors: RW
authors: Hammond
authors: P
authors: P
authors: Ramirez
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002213:Capsid / Q000737:chemistry / Q000235:genetics; D003001:Cloning, Molecular; D019143:Evolution, Molecular; D005814:Genes, Viral / Q000235:genetics; D016679:Genome, Viral; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016366:Open Reading Frames / Q000235:genetics; D010942:Plant Viruses / Q000737:chemistry / Q000235:genetics*; D020815:Polyproteins / Q000737:chemistry / Q000235:genetics; D012328:RNA Viruses / Q000737:chemistry / Q000235:genetics*; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics; D016415:Sequence Alignment; D017385:Sequence Homology; D017806:Tymovirus / Q000737:chemistry; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics; D003313:Zea mays / Q000821:virology*
chemical_list: D020815:Polyproteins; D012367:RNA, Viral; D014764:Viral Proteins