Chemical synthesis of lymphotactin: a glycosylated chemokine with a C-terminal mucin-like domain.
The synthesis of a 93-residue chemokine, lymphotactin, containing eight sites of O-linked glycosylation, was achieved using the technique of native chemical ligation. A single GalNAc residue was incorporated at each glycosylation site using standard Fmoc-chemistry to achieve the first total synthesis of a mucin-type glycoprotein. Using this approach quantities of homogeneous material were obtained for structural and functional analysis.
Marcaurelle
L A
LA
Department of Chemistry, Howard Hughes Medical Institute, University of California, Berkeley 94720, USA.
Mizoue
L S
LS
Wilken
J
J
Oldham
L
L
Kent
S B
SB
Handel
T M
TM
Bertozzi
C R
CR
D000595:Amino Acid Sequence; D001671:Biochemistry / Q000379:methods*; D002478:Cells, Cultured; D019744:Chemokines, C; D006031:Glycosylation; D006801:Humans; D007668:Kidney / Q000166:cytology; D008222:Lymphokines / Q000138:chemical synthesis* / Q000378:metabolism / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D011487:Protein Conformation; D011956:Receptors, Cell Surface / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled; D012795:Sialoglycoproteins / Q000138:chemical synthesis* / Q000378:metabolism / Q000494:pharmacology
D019744:Chemokines, C; D008222:Lymphokines; D008565:Membrane Proteins; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; D012795:Sialoglycoproteins; C092390:XCL1 protein, human; C091671:XCR1 protein, human; C018997:lymphotactin