T cytotoxic 1 and T cytotoxic 2 CD8 T cells both inhibit IgE responses.
BACKGROUND
It is well recognized that CD8 T cells inhibit IgE responses. In this study, we investigated the mechanism of CD8 T cell-mediated IgE suppression by comparing the capacity of T cytotoxic 1 (Tc1) and T cytotoxic 2 (Tc2) CD8 T cells to inhibit IgE responses to ovalbumin (OVA).


METHODS
Tc1 and Tc2 CD8 T cells were generated from OVA(257-264)-specific Vbeta5.2 T cell receptor (TcR) transgenic mice by stimulation with anti-CD3 and anti-CD28 under Tc1 and Tc2 polarizing conditions. Tc1 and Tc2 Vbeta5.2 TcR CD8 T cells (10(6)) were adoptively transferred to syngeneic mice, and following immunization with 100 micro of OVA/alum, serum IgE antibodies were measured by passive cutaneous anaphylaxis and expressed as the highest dilution that gave a detectable skin response.


RESULTS
Both Tc1 and Tc2 CD8 T cells from OT-I mice inhibited IgE.


CONCLUSIONS
Both Tc1 and Tc2 CD8 T cells promote Th1 immunity and inhibit IgE responses. This process appears to be independent of CD8 T cell-derived IFN-gamma, as both Tc2 (IFN-gamma-) and Tc1 (IFN-gamma+) CD8 T cells inhibited IgE.
Thomas
M J
MJ
Department of Immunology, Guy's, King's and St Thomas's School of Medicine and Dentistry, Kings College, London, UK.
MacAry
P A
PA
Noble
A
A
Askenase
P W
PW
Kemeny
D M
DM
D019264:Adoptive Transfer; D000485:Allergens / Q000276:immunology; D000818:Animals; D002478:Cells, Cultured; D019672:Genes, T-Cell Receptor; D007073:Immunoglobulin E / Q000096:biosynthesis*; D007135:Immunoglobulin Variable Region / Q000235:genetics; D007371:Interferon-gamma / Q000096:biosynthesis; D015847:Interleukin-4 / Q000096:biosynthesis; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D010047:Ovalbumin / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000145:classification / Q000276:immunology* / Q000637:transplantation
D000485:Allergens; D007135:Immunoglobulin Variable Region; D015847:Interleukin-4; D007073:Immunoglobulin E; D007371:Interferon-gamma; D010047:Ovalbumin