title: Liver metastasis following pneumoperitoneum with different gases in a mouse model.
abstract: BACKGROUND
  The validity of using CO2 in laparoscopic tumor surgery has not yet been established. To address this question, we investigated the growth of liver metastases following insufflation with different gases in a mouse laparoscopy model.
  
  
  METHODS
  Male BALB/C mice inoculated intraportally with colon 26 cells were randomized to undergo pneumoperitoneum with CO2 (n = 16), helium (n = 16), argon (n = 16), or air (n = 17), or to act as controls without insufflation (n = 17).
  
  
  RESULTS
  The growth of cancer nodules on the liver 14 days after surgery was greater in mice following insufflation with CO2 (p < 0.01), helium (p < 0.01), argon (p = 0.01), and air (p = 0.07) than in control mice. No significant differences were found between the four insufflation groups in the growth of liver metastases.
  
  
  CONCLUSIONS
  These results suggest that insufflation plays an important role in the development of liver metastases but that the choice of gas may not affect their growth.
authors: Department of Surgery, Saitama Medical Center, Saitama Medical School, 1981 Kamoda Kawagoe, Saitama 350-8550, Japan.
authors: H
authors: H
authors: Ishida
authors: Y
authors: Y
authors: Idezuki
authors: M
authors: M
authors: Yokoyama
authors: H
authors: H
authors: Nakada
authors: A
authors: A
authors: Odaka
authors: N
authors: N
authors: Murata
authors: M
authors: M
authors: Fujioka
authors: D
authors: D
authors: Hashimoto
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001128:Argon / Q000009:adverse effects; D015179:Colorectal Neoplasms / Q000473:pathology / Q000601:surgery*; D004195:Disease Models, Animal; D006371:Helium / Q000009:adverse effects; D010535:Laparoscopy / Q000009:adverse effects* / Q000379:methods; D008113:Liver Neoplasms / Q000209:etiology* / Q000473:pathology* / Q000556:secondary*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009366:Neoplasm Seeding; D010100:Oxygen / Q000009:adverse effects; D011028:Pneumoperitoneum, Artificial / Q000009:adverse effects* / Q000379:methods; D011336:Probability; D018570:Risk Assessment
chemical_list: D006371:Helium; D001128:Argon; D010100:Oxygen