title: Donors of NO and pulsed radiation at lambda = 820 nm exert effects on cell attachment to extracellular matrices.
abstract: The adhesion of HeLa cells to a glass matrix was evaluated after the irradiation of the cell suspension with a pulsed near-infrared light-emitting diode (lambda = 820 nm, frequency 10 Hz, dose 8-120 J/m(2)) and treatment with two donors of nitric oxide, sodium nitroprusside (SNP, 5 x 10(-4) M) and NaNO(2) (4 x 10(-4) M). It was found that in an irradiated cell suspension, the cell-glass adhesion increases in a dose-dependent manner (a bell-shaped curve with a maximum at 60 J/m(2)). The treatment of cells with SNP or NaNO(2) before the irradiation eliminates the radiation-induced attachment stimulation. Pretreatment of cells with SNP not only eliminates the radiation-induced attachment stimulation but inhibits the attachment of irradiated (but not non-irradiated) cells. It is suggested that a modulation of the activity of respiratory chain (probably the alteration of the activity of cytochrome c oxidase) is involved in radiation-induced increase of cell attachment.
authors: Institute of Laser and Informatic Technologies, Russian Academy of Sciences, 142190, Moscow Region, Troitsk, Russia. karu@isan.troitsk.ru
authors: T I
authors: TI
authors: Karu
authors: L V
authors: LV
authors: Pyatibrat
authors: G S
authors: GS
authors: Kalendo
mesh_terms: D002448:Cell Adhesion / Q000187:drug effects* / Q000528:radiation effects*; D005109:Extracellular Matrix / Q000187:drug effects* / Q000528:radiation effects*; D005720:Gamma Rays; D006367:HeLa Cells; D006801:Humans; D007202:Indicators and Reagents; D008027:Light; D009130:Muscle, Smooth / Q000166:cytology* / Q000187:drug effects; D009566:Nitrates / Q000494:pharmacology; D020030:Nitric Oxide Donors / Q000494:pharmacology*; D009599:Nitroprusside / Q000494:pharmacology
chemical_list: D007202:Indicators and Reagents; D009566:Nitrates; D020030:Nitric Oxide Donors; D009599:Nitroprusside; C031618:sodium nitrate