[Cytotoxic activity and cytokine gene induction of Asp-hemolysin to vascular endothelial cells].
We examined the effects of Asp-hemolysin from Aspergillus fumigatus Fresenius-Muramatsu strain on the viability and cytokine gene expression of human umbilical vein endothelial cells (HUVEC). The cell viability of HUVEC was reduced to 50% by 100 micrograms/ml of Asp-hemolysin. However, lower concentration of Asp-hemolysin (< 30 micrograms/ml) had no effect on the cell viability. The mRNA expression of such cytokines as tumor necrosis factor-alpha (TNF-alpha), interleukin (IL)-1 beta, IL-6, IL-8 and granulocyte-macrophage colony stimulating factor (GM-CSF) were also observed in HUVEC cultured with 30 micrograms/ml of Asp-hemolysin.
Kumagai
T
T
First Department of Hygienic Chemistry, Tohoku Pharmaceutical University, 4-4-1 Komatsushima, Aoba-ku, Sendai 981-8558, Japan.
Nagata
T
T
Kudo
Y
Y
Fukuchi
Y
Y
Ebina
K
K
Yokota
K
K
D002478:Cells, Cultured; D016207:Cytokines / Q000235:genetics* / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects*; D005656:Fungal Proteins / Q000494:pharmacology*; D006460:Hemolysin Proteins / Q000494:pharmacology*; D006801:Humans; D012333:RNA, Messenger / Q000032:analysis
C089497:Asp-HS protein, Aspergillus fumigatus; D016207:Cytokines; D005656:Fungal Proteins; D006460:Hemolysin Proteins; D012333:RNA, Messenger