Method developments for iodine speciation by reversed-phase liquid chromatography-ICP-mass spectrometry.
Method developments are described for a iodine speciation method. This method is based on a reversed-phase liquid chromatography separation combined with inductively coupled plasma-mass spectrometry detection. Investigations are performed concerning the methanol tolerance/stability of inductively coupled plasma, postcolumn oxidation/volatilization of iodine from species, influences of buffer pH, and column temperature on separation efficiency. The experiments focused on six iodine species, namely iodine, monoiodothyrosine, di-iodothyrosine, tri-iodothyronine, reversed tri-iodothyronine, and thyroxin. Detection limits were determined between 0.08 and 1.5 microg/L (iodine related), dependent on species. Appplications on a urine sample were performed, showing iodide as the predominant iodine species.
Michalke
B
B
GSF National Research Center for Environment and Health, Institute for Ecological Chemistry, Neuherberg, Germany.
Schramel
P
P
Witte
H
H
D002021:Buffers; D002138:Calibration; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D004105:Diiodotyrosine / Q000032:analysis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007455:Iodine / Q000032:analysis* / Q000652:urine; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D000432:Methanol / Q000737:chemistry; D013696:Temperature
D002021:Buffers; D004105:Diiodotyrosine; D007455:Iodine; D000432:Methanol