Echoviruses bind heparan sulfate at the cell surface.
Some echoviruses (EV) that bind decay-accelerating factor (DAF) also bind cells of human and murine origins in a DAF-independent manner. Pretreatment of cells with heparinase 1 or heparin blocks the binding of radiolabeled virus to the cell surface, and heparin prevents infection of rhabdomyosarcoma cells by certain EV, including several low-passage clinical isolates of EV 6 and some EV that do not bind DAF. These studies suggest that heparan sulfate may be of in vivo relevance as an attachment molecule for EV.
Goodfellow
I G
IG
Division of Virology, Institute of Biomolecular and Life Sciences, University of Glasgow, Glasgow, United Kingdom.
Sioofy
A B
AB
Powell
R M
RM
Evans
D J
DJ
D016475:3T3 Cells; D000818:Animals; D018958:CD55 Antigens / Q000235:genetics / Q000502:physiology; D016466:CHO Cells; D002462:Cell Membrane / Q000378:metabolism; D006224:Cricetinae; D029822:Enterovirus B, Human / Q000378:metabolism*; D006493:Heparin / Q000031:analogs & derivatives / Q000378:metabolism; D019763:Heparin Lyase / Q000378:metabolism; D006497:Heparan Sulfate / Q000378:metabolism*; D006801:Humans; D051379:Mice; D011991:Receptors, Virus / Q000378:metabolism*; D010738:Type C Phospholipases / Q000378:metabolism
D018958:CD55 Antigens; D006493:Heparin; D019763:Heparin Lyase; D006497:Heparan Sulfate; D011991:Receptors, Virus; D010738:Type C Phospholipases; C044153:heparin, N-desulfated