title: SDF-1 induces IL-8 production and transendothelial migration of human cord blood-derived mast cells.
abstract: BACKGROUND
  Mast cell numbers and expression of chemokines are known to increase in the context of angiogenesis and inflammation, but the mechanisms by which this occurs are not understood. Stromal-derived factor-1 (SDF-1) is an important chemokine in angiogenesis and cell migration. The effects of SDF-1 on human mast cells were examined.
  
  
  METHODS
  Expression of the SDF-1 receptor CXC chemokine receptor 4 (CXCR4) on mast cells was examined by RT-PCR and flow cytometry. The ability of labeled cord blood-derived mast cells to migrate across HUVEC monolayers in response to SDF-1 was determined. The cytokine and chemokine responses of cord blood-derived mast cells to SDF-1 treatment over 24 h were examined by ELISA.
  
  
  RESULTS
  Cord blood-derived human mast cells expressed the CXCR4 receptor for SDF-1 and migrated across HUVEC monolayers in response to this chemokine. Treatment of cord blood-derived mast cells with SDF-1 did not induce degranulation or the production of several cytokines but did induce a highly selective IL-8 response.
  
  
  CONCLUSIONS
  Human mast cells can both migrate across vascular endothelium and produce the pro-angiogenic chemokine IL-8 in response to SDF-1. These responses may be important in angiogenic processes.
authors: Departments of Pathology, Microbiology and Immunology and Pediatrics, Dalhousie University, Halifax, Canada.
authors: T J
authors: TJ
authors: Lin
authors: T B
authors: TB
authors: Issekutz
authors: J S
authors: JS
authors: Marshall
mesh_terms: D002465:Cell Movement; D002478:Cells, Cultured; D054377:Chemokine CXCL12; D019743:Chemokines, CXC / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000378:metabolism*; D005312:Fetal Blood / Q000166:cytology; D006801:Humans; D016209:Interleukin-8 / Q000096:biosynthesis*; D008407:Mast Cells / Q000378:metabolism / Q000502:physiology*; D019718:Receptors, CXCR4 / Q000096:biosynthesis / Q000235:genetics
chemical_list: C515255:CXCL12 protein, human; D054377:Chemokine CXCL12; D019743:Chemokines, CXC; D016209:Interleukin-8; D019718:Receptors, CXCR4