title: Regulation by phagic T-lymphocytes of a (pluripotent?) organ stem cell present in adult human blood. A beneficial exception to self-tolerance.
abstract: Stem cells isolated from adult human blood are able to give rise to several different kinds of cell types such as mesenchymal cells, including striated muscle cells, hepatocytes, and endothelial-cells. Because independently studied by authors whose interests focused on particular tissue types, these stem cells have been described as different. However, they might well represent one unique population of pluripotent stem cells in homeostatic equilibrium with the 'reserve' stem cells buried in organs. In the blood, these stem cells have a monocytic phenotype. In in vitro culture, once they have adhered, they spontaneously differentiate into diverse types of cells reminiscent of embryonic stem cells in culture. Normally, they are almost quiescent cells. But under precise circumstances such as wound-healing, they may proliferate and migrate to the right organ to give rise there to the right type of cells, in order to participate in the repair process. Indeed, such a powerful stem cell needs to be tightly controlled. We illustrate here, by time-lapse videocinematography, how a special subpopulation of T-lymphocytes, for which we coined the name 'phagic T-lymphocytes' (PTLs), destroys these stem cells as soon as they differentiate in vitro, i.e., without the purpose of a repair. These stem cells express constitutively HLA-DR molecules and therefore can act as antigen-presenting cells able to activate phagic T-lymphocytes. The targets of these activated phagic T-lymphocytes are the differentiated stem cell themselves. Phagic T-lymphocytes are attracted by the stem cells, circulate around them, then penetrate and circulate inside them until the latter 'explode'. This mechanism of destruction by phagic T-lymphocytes is unique and seems to be normally restricted to stem cells. It represents a beneficial exception in self-tolerance since it avoids the accumulation of these stem cells out of healing purposes. Interestingly, in disorders such as fibrosis and/or some malignant proliferations, these stem cells proliferate, escape destruction by phagic T-lymphocytes and, as a consequence, accumulate, giving rise to a 'tissue' when cultured in vitro.
authors: UMR 956 INRA/AFSSA/ENVA, Ecole nationale vétérinaire d'Alfort, Maisons-Alfort, France. marielouise.labat@free.fr
authors: M L
authors: ML
authors: Labat
authors: M
authors: M
authors: Pouchelet
authors: N
authors: N
authors: Gouhier
authors: P
authors: P
authors: Boireau
authors: G
authors: G
authors: Milhaud
mesh_terms: D000328:Adult; D002454:Cell Differentiation; D002478:Cells, Cultured; D006801:Humans; D008855:Microscopy, Electron, Scanning; D018715:Microscopy, Video; D010587:Phagocytosis; D013234:Stem Cells / Q000502:physiology*; D013601:T-Lymphocytes / Q000502:physiology*