Distal ligand reactivity and quaternary structure studies of proximally detached hemoglobins.
The linkage between the proximal histidines and the proximal polypeptide in normal adult human hemoglobin (Hb A) has been proposed to play a major role in transmitting allosteric effects between oxygen binding sites [Perutz, M. F. (1970) Nature 228, 726-734]. Here we present circular dichroism (CD), (1)H NMR, analytical ultracentrifugation, and stopped-flow kinetic data to better define the quaternary structure of hemoglobins in which the linkage between the proximal histidines and the polypeptide backbone has been broken [Barrick et al. Nat. Struct. Biol. 4, 78-83 (1997)] and to characterize the distal ligand binding properties of these proximally detached Hbs. CD spectroscopy indicates that rHb (alphaH87G) and rHb (alphaH87G/betaH92G) retain at least partial T-quaternary structure with distal ligand bound, whereas rHb (betaH92G) does not, consistent with (1)H NMR spectra. Analytical ultracentrifugation reveals significant tetramer dissociation in rHb (betaH92G) to be the likely cause of loss of T-state markers. These quaternary structure studies indicate that in distally liganded Hb, the T-state is compatible with proximal linkages in the beta- but not the alpha-chains. (1)H NMR titrations of rHb (alphaH87G) with n-butyl isocyanide demonstrate the alpha-chains to be of high affinity as compared with the beta-chains. Comparing ligand association and dissociation rates between the rHb (alphaH87G) variant with the T- and R-states of wild-type Hb A indicates that at the alpha-chains, carbon monoxide affinity is modulated entirely by the proximal linkage, rather than from distal interactions. Some residual allosteric interactions may remain operative at the beta-chains of rHb (alphaH87G).
Barrick
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T. C. Jenkins Department of Biophysics, Johns Hopkins University, Baltimore Maryland 21218, USA.
Ho
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Simplaceanu
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Ho
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D000494:Allosteric Regulation; D001665:Binding Sites; D002248:Carbon Monoxide / Q000737:chemistry; D002263:Carboxyhemoglobin / Q000737:chemistry; D002942:Circular Dichroism; D005914:Globins / Q000737:chemistry / Q000235:genetics; D006441:Hemoglobin A / Q000737:chemistry; D006454:Hemoglobins / Q000737:chemistry* / Q000235:genetics; D006801:Humans; D008024:Ligands; D009570:Nitriles / Q000737:chemistry; D019906:Nuclear Magnetic Resonance, Biomolecular; D010833:Phytic Acid / Q000737:chemistry; D020836:Protein Structure, Quaternary; D011994:Recombinant Proteins / Q000737:chemistry; D013329:Structure-Activity Relationship; D014461:Ultracentrifugation
D006454:Hemoglobins; D008024:Ligands; D009570:Nitriles; D011994:Recombinant Proteins; C002990:butyl isocyanide; D010833:Phytic Acid; D002248:Carbon Monoxide; D005914:Globins; C100003:deoxyhemoglobin; D006441:Hemoglobin A; D002263:Carboxyhemoglobin