title: Flow-induced dilation of human coronary arterioles: important role of Ca(2+)-activated K(+) channels.
abstract: BACKGROUND
  Flow-induced vasodilation (FID) is a physiological mechanism for regulating coronary flow and is mediated largely by nitric oxide (NO) in animals. Because hyperpolarizing mechanisms may play a greater role than NO in the microcirculation, we hypothesized that hyperpolarization contributes importantly to FID of human coronary arterioles.
  
  
  RESULTS
  Arterioles from atria or ventricles were cannulated for videomicroscopy. Membrane potential of vascular smooth muscle cells (VSMCs) was measured simultaneously. After constriction with endothelin-1, increases in flow induced an endothelium-dependent vasodilation. Nomega-Nitro-L-arginine methyl ester 10(-4) mol/L modestly impaired FID of arterioles from patients without coronary artery disease (CAD), whereas no inhibition was seen in arterioles from patients with CAD. Indomethacin 10(-5) mol/L was without effect, but 40 mmol/L KCl attenuated maximal FID. Tetraethylammonium 10(-3) mol/L but not glibenclamide 10(-6) mol/L reduced FID. Charybdotoxin 10(-8) mol/L impaired both FID (15+/-3% versus 75+/-12%, P<0.05) and hyperpolarization (-32+/-2 mV [from -28+/-2 mV after endothelin-1] versus -42+/-2 mV [-27+/-2 mV], P<0.05). Miconazole 10(-6) mol/L or 17-octadecynoic acid 10(-5) mol/L reduced FID. By multivariate analysis, age was an independent predictor for the reduced FID. Conclusions-We conclude that shear stress induces endothelium-dependent vasodilation, hyperpolarizing VSMCs through opening Ca(2+)-activated K(+) channels in human coronary arterioles. In subjects without CAD, NO contributes to FID. NO and prostaglandins play no role in patients with CAD; rather, cytochrome P450 metabolites are involved. This is consistent with a role for endothelium-derived hyperpolarizing factor in FID of the human coronary microcirculation.
authors: VA Medical Center, the Department of Medicine, and the Cardiovascular Research Center, Medical College of Wisconsin, Milwaukee, USA.
authors: H
authors: H
authors: Miura
authors: R E
authors: RE
authors: Wachtel
authors: Y
authors: Y
authors: Liu
authors: F R
authors: FR
authors: Loberiza
authors: T
authors: T
authors: Saito
authors: M
authors: M
authors: Miura
authors: D D
authors: DD
authors: Gutterman
mesh_terms: D000367:Age Factors; D001160:Arterioles / Q000187:drug effects / Q000502:physiology*; D001783:Blood Flow Velocity / Q000502:physiology; D002118:Calcium / Q000378:metabolism*; D018999:Charybdotoxin / Q000494:pharmacology; D003331:Coronary Vessels / Q000187:drug effects / Q000502:physiology*; D019332:Endothelin-1 / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology; D005260:Female; D005905:Glyburide / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D008825:Miconazole / Q000494:pharmacology; D008875:Middle Aged; D015999:Multivariate Analysis; D026902:Potassium Channel Blockers; D015221:Potassium Channels / Q000378:metabolism*; D011189:Potassium Chloride / Q000494:pharmacology; D013314:Stress, Mechanical; D019789:Tetraethylammonium / Q000494:pharmacology; D014664:Vasodilation / Q000187:drug effects / Q000502:physiology*
chemical_list: D019332:Endothelin-1; D004791:Enzyme Inhibitors; D005231:Fatty Acids, Unsaturated; D026902:Potassium Channel Blockers; D015221:Potassium Channels; D018999:Charybdotoxin; C047008:17-octadecynoic acid; D019789:Tetraethylammonium; D011189:Potassium Chloride; D008825:Miconazole; D005905:Glyburide; D002118:Calcium