Platelet storage in PAS-2 or autologous plasma: impact on functional parameters.
Currently, several platelet additive solutions for long-term platelet storage have been introduced. The aim of this study was to compare the deterioration of functional status of platelets stored for up to 5 days in autologous plasma (AP) only, with platelet stored in PAS-2, a salt solution containing acetate, citrate and sodium chloride. Change in platelet adhesion, aggregation and activation was measured by flow cytometric technique. In addition, beta-Thromboglobulin (beta-TG), lactate and glucose were determined. After 5 days of storage, the expression of P-Selectin was significantly higher, the production of lactate and the consumption of glucose were significantly lower, in platelets stored in PAS-2 than in autologous plasma. No significant differences were detected on day 5 between the two groups with regard to fibrinogen, von Willebrand factor binding capacity, or to beta-TG release. It can be concluded that neither storage medium was consistently better for the parameters tested. However, it must be emphasized that platelets stored in autologous plasma exhibited less lesion, in terms of P-Selectin expression compared with platelets stored in PAS-2.
Bunescu
A
A
Department of Clinical Chemistry, Nova Medical St Göran's Hospital, Stockholm, Sweden.
Hild
M
M
Lundahl
J
J
Egberg
N
N
D000085:Acetates / Q000494:pharmacology*; D001792:Blood Platelets; D001793:Blood Preservation / Q000379:methods* / Q000592:standards; D002951:Citrates / Q000494:pharmacology*; D019343:Citric Acid / Q000494:pharmacology; D005340:Fibrinogen / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D019007:P-Selectin / Q000378:metabolism; D010949:Plasma; D015539:Platelet Activation; D010979:Platelet Function Tests; D011310:Preservatives, Pharmaceutical / Q000494:pharmacology*; D011485:Protein Binding; D012965:Sodium Chloride / Q000494:pharmacology*; D001620:beta-Thromboglobulin / Q000378:metabolism; D014841:von Willebrand Factor / Q000378:metabolism
D000085:Acetates; D002951:Citrates; D019007:P-Selectin; C436265:PAS-2 salt solution; D011310:Preservatives, Pharmaceutical; D001620:beta-Thromboglobulin; D014841:von Willebrand Factor; D019343:Citric Acid; D012965:Sodium Chloride; D005340:Fibrinogen