Cellular uptake of taurine by lactating porcine mammary tissue.
Milk taurine plays a critical role in neonatal development. Taurine uptake in lactating sow mammary tissue has not been characterized previously. The kinetic properties, ion dependence and substrate specificity of taurine uptake were characterized in mammary tissue collected from lactating sows at slaughter. Tissue explants were incubated in an isosmotic physiologic buffer with [3H]taurine tracer to measure taurine uptake. Taurine uptake was dependent upon the presence of extracellular sodium and chloride ions, which is consistent with the co-transport of sodium and chloride with taurine. Uptake was not dependent upon ion exchange mechanisms or upon furosemide-sensitive ion co-transport. Taurine uptake was saturable and exhibited an apparent Km of 20 microM and a V(max) of 386 micromol/kg cell water/30 min. Substrate specificity studies indicated a strong interaction of beta-amino acids with the taurine transport system. Taurine transport in lactating sow mammary tissue is therefore a high affinity, sodium-dependent mechanism specific for beta-amino acids, and is analogous to sodium-dependent taurine uptake in other tissues. The high affinity and high specificity of the taurine uptake system allows for concentration of taurine within the mammary cell and is ultimately responsible for provision of taurine required for neonatal development.
Bryson
J M
JM
Department of Animal Sciences, University of Illinois, Urbana, IL 61801, USA.
Jackson
S C
SC
Wang
H
H
Hurley
W L
WL
D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D018929:Cell Culture Techniques; D002713:Chlorine / Q000494:pharmacology; D005260:Female; D007477:Ions / Q000494:pharmacology; D007700:Kinetics; D007774:Lactation / Q000378:metabolism*; D008321:Mammary Glands, Animal / Q000166:cytology / Q000378:metabolism*; D012964:Sodium / Q000494:pharmacology; D013379:Substrate Specificity; D013552:Swine / Q000378:metabolism* / Q000502:physiology; D013654:Taurine / Q000493:pharmacokinetics*; D014316:Tritium
D007477:Ions; D014316:Tritium; D013654:Taurine; D002713:Chlorine; D012964:Sodium