Hematopoietic progenitor cell counts performed by the Sysmex SE-9000 analyzer can guide timing of peripheral blood stem cell harvest.
BACKGROUND
We studied whether the hematopoietic progenitor cell (HPC) count in peripheral blood as evaluated by an automated counter, the Sysmex SE-9000, correlated with CD34 positive (+) cell count and therefore could guide the timing of peripheral blood stem cell (PBSC) harvest.


METHODS
HPC count and flow cytometric CD34+ cell count were measured in 90 peripheral blood samples and 30PBSC samples. The correlation between HPC count and apheretic CD34+ cell yield was examined in 19 patients.


RESULTS
HPC count showed good correlations with CD34+ cell count in peripheral blood (r = 0.699) and PBSC (r = 0.892). The correlation between peripheral blood HPC count and apheretic CD34+ cell yield also was good (r = 0.789).


CONCLUSIONS
Automated HPC counting can be used as a screening test to guide the timing of PBSC harvest.
Endoh
A
A
Division of Laboratory Diagnosis, Sapporo Medical University School of Medicine, South-1, West-16, Chuo-ku, Sapporo 060-8543, Japan.
Yagihashi
A
A
Asanuma
K
K
Moriai
R
R
Izawa
A
A
Koyanagi
Y
Y
Sato
T
T
Kobayashi
D
D
Watanabe
N
N
D000328:Adult; D018952:Antigens, CD34 / Q000032:analysis; D001772:Blood Cell Count / Q000295:instrumentation*; D001781:Blood Component Removal / Q000379:methods*; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology; D019650:Hematopoietic Stem Cell Mobilization; D006412:Hematopoietic Stem Cells; D006801:Humans; D008297:Male; D013584:Sarcoma, Synovial / Q000097:blood; D013997:Time Factors; D014594:Uterine Neoplasms / Q000097:blood
D018952:Antigens, CD34; D016179:Granulocyte Colony-Stimulating Factor