Genetic markers from Biomphalaria tenagophila (Gastropoda: Pulmonata: Planorbidae) obtained by the double stringency polymerase chain reaction technique.
Biomphalaria tenagophila, one of the intermediate hosts of the trematoda Schistosoma mansoni, is a simultaneous hermafrodite snail species. In order to analyse the genetic structure of these populations, we performed a double-stringency PCR technique to obtain genetic markers with microsatellites and arbitrary primers in a single reaction.
Tuan
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Laboratório de Malacologia, Superintendência de Controle de Endemias, São Paulo, SP, 01027-000, Brasil. tuan@sucen.sp.gov.br
Bortolato
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D000818:Animals; D001700:Biomphalaria / Q000235:genetics*; D017931:DNA Primers / Q000032:analysis; D005819:Genetic Markers; D018895:Microsatellite Repeats / Q000235:genetics; D008970:Molecular Weight; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic
D017931:DNA Primers; D005819:Genetic Markers