SDF-1 induces IL-8 production and transendothelial migration of human cord blood-derived mast cells.
BACKGROUND
Mast cell numbers and expression of chemokines are known to increase in the context of angiogenesis and inflammation, but the mechanisms by which this occurs are not understood. Stromal-derived factor-1 (SDF-1) is an important chemokine in angiogenesis and cell migration. The effects of SDF-1 on human mast cells were examined.


METHODS
Expression of the SDF-1 receptor CXC chemokine receptor 4 (CXCR4) on mast cells was examined by RT-PCR and flow cytometry. The ability of labeled cord blood-derived mast cells to migrate across HUVEC monolayers in response to SDF-1 was determined. The cytokine and chemokine responses of cord blood-derived mast cells to SDF-1 treatment over 24 h were examined by ELISA.


RESULTS
Cord blood-derived human mast cells expressed the CXCR4 receptor for SDF-1 and migrated across HUVEC monolayers in response to this chemokine. Treatment of cord blood-derived mast cells with SDF-1 did not induce degranulation or the production of several cytokines but did induce a highly selective IL-8 response.


CONCLUSIONS
Human mast cells can both migrate across vascular endothelium and produce the pro-angiogenic chemokine IL-8 in response to SDF-1. These responses may be important in angiogenic processes.
Lin
T J
TJ
Departments of Pathology, Microbiology and Immunology and Pediatrics, Dalhousie University, Halifax, Canada.
Issekutz
T B
TB
Marshall
J S
JS
D002465:Cell Movement; D002478:Cells, Cultured; D054377:Chemokine CXCL12; D019743:Chemokines, CXC / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000378:metabolism*; D005312:Fetal Blood / Q000166:cytology; D006801:Humans; D016209:Interleukin-8 / Q000096:biosynthesis*; D008407:Mast Cells / Q000378:metabolism / Q000502:physiology*; D019718:Receptors, CXCR4 / Q000096:biosynthesis / Q000235:genetics
C515255:CXCL12 protein, human; D054377:Chemokine CXCL12; D019743:Chemokines, CXC; D016209:Interleukin-8; D019718:Receptors, CXCR4