title: Coupling of voltage-dependent potassium channel inactivation and oxidoreductase active site of Kvbeta subunits.
abstract: The accessory beta subunits of voltage-dependent potassium (Kv) channels form tetramers arranged with 4-fold rotational symmetry like the membrane-integral and pore-forming alpha subunits (Gulbis, J. M., Mann, S., and MacKinnon, R. (1999) Cell. 90, 943-952). The crystal structure of the Kvbeta2 subunit shows that Kvbeta subunits are oxidoreductase enzymes containing an active site composed of conserved catalytic residues, a nicotinamide (NADPH)-cofactor, and a substrate binding site. Also, Kvbeta subunits with an N-terminal inactivating domain like Kvbeta1.1 (Rettig, J., Heinemann, S. H., Wunder, F., Lorra, C., Parcej, D. N., Dolly, O., and Pongs, O. (1994) Nature 369, 289-294) and Kvbeta3.1 (Heinemann, S. H., Rettig, J., Graack, H. R., and Pongs, O. (1996) J. Physiol. (Lond.) 493, 625-633) confer rapid N-type inactivation to otherwise non-inactivating channels. Here we show by a combination of structural modeling and electrophysiological characterization of structure-based mutations that changes in Kvbeta oxidoreductase activity may markedly influence the gating mode of Kv channels. Amino acid substitutions of the putative catalytic residues in the Kvbeta1.1 oxidoreductase active site attenuate the inactivating activity of Kvbeta1.1 in Xenopus oocytes. Conversely, mutating the substrate binding domain and/or the cofactor binding domain rescues the failure of Kvbeta3.1 to confer rapid inactivation to Kv1.5 channels in Xenopus oocytes. We propose that Kvbeta oxidoreductase activity couples Kv channel inactivation to cellular redox regulation.
authors: Institut für Neurale Signalverarbeitung, Zentrum für Molekulare Neurobiologie der Universität Hamburg, Hamburg, Germany.
authors: R
authors: R
authors: Bähring
authors: C J
authors: CJ
authors: Milligan
authors: V
authors: V
authors: Vardanyan
authors: B
authors: B
authors: Engeland
authors: B A
authors: BA
authors: Young
authors: J
authors: J
authors: Dannenberg
authors: R
authors: R
authors: Waldschutz
authors: J P
authors: JP
authors: Edwards
authors: D
authors: D
authors: Wray
authors: O
authors: O
authors: Pongs
mesh_terms: D000818:Animals; D001665:Binding Sites; D016466:CHO Cells; D002384:Catalysis; D006224:Cricetinae; D015640:Ion Channel Gating; D051662:Kv1.1 Potassium Channel; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D010088:Oxidoreductases / Q000378:metabolism*; D026902:Potassium Channel Blockers; D015221:Potassium Channels / Q000737:chemistry / Q000378:metabolism*; D024642:Potassium Channels, Voltage-Gated; D051381:Rats; D017386:Sequence Homology, Amino Acid; D014981:Xenopus
chemical_list: D026902:Potassium Channel Blockers; D015221:Potassium Channels; D024642:Potassium Channels, Voltage-Gated; D051662:Kv1.1 Potassium Channel; D010088:Oxidoreductases