[Monitoring of oxidative stress after experimental small bowel autotransplantation].
The difficulty at transplanting the small bowel mainly is caused by the biology of the intestine. It is highly immunogenic, is one of the most sensitive tissues to ischemia-reperfusion injury. Our aims were to investigate changes of oxygen free radical mediated reactions after autotransplantation at different preservation times and perfusion fluids. Our results prove that this model is feasible to examine ischemia-reperfusion injury in the small intestine. Euro Collins (EC) is a suitable preserving solution for small bowel transplantation. There was no significant lipid peroxidation within the first 6 hours of graft preservation. However superoxide dismutase (SOD) activity was dramatically reduced during reperfusion in the tissues samples. Significant increase of reduced glutathione at the same time can be explained by compensatory mechanism to neutralize increased free radical production.
Ferencz
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Pécsi Tudományegyetem Altalános Orvostudományi Kar Kísérletes Sebészeti Intézet. andrea@expsurg.pote.hu
Tavakoli
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Kalmár-Nagy
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K
Szántó
Z
Z
Röth
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E
Horváth
O P
OP
D000818:Animals; D004285:Dogs; D005609:Free Radicals / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D066298:In Vitro Techniques; D007421:Intestine, Small / Q000098:blood supply / Q000201:enzymology / Q000378:metabolism* / Q000637:transplantation*; D017078:Laser-Doppler Flowmetry; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008833:Microcirculation; D018384:Oxidative Stress; D015427:Reperfusion Injury / Q000209:etiology / Q000378:metabolism*; D013152:Splanchnic Circulation; D013482:Superoxide Dismutase / Q000378:metabolism; D014182:Transplantation, Autologous
D005609:Free Radicals; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D005978:Glutathione