title: Rhizobia of chickpea from southern Portugal: symbiotic efficiency and genetic diversity.
abstract: OBJECTIVE
  In order to evaluate differences between chickpea rhizobial populations from three geographical areas in southern Portugal (Beja, Elvas and Evora), isolates from the three regions were obtained and analysed.
  
  
  RESULTS
  The genetic characterization of the isolates was done by plasmid profiles and restriction analysis of the nifH gene. Symbiotic efficiency of the isolates was also determined. Relationships between geographical origin, symbiotic efficiency and molecular characteristics were established. Beja soil revealed a larger rhizobia population as well as the presence of some of the isolates with higher symbiotic efficiency values. Isolates with a single plasmid showed a significantly higher symbiotic efficiency.
  
  
  CONCLUSIONS
  Genetic and phenotypic differences were detected between the natural rhizobial populations from the three locations.
  
  
  CONCLUSIONS
  The different yield potential with cultivars of chickpea usually obtained in the three regions of southern Portugal could be due to their different natural rhizobial populations.
authors: Departamento de Biologia, Universidade de Evora, 7002-554 Evora, Portugal.
authors: M
authors: M
authors: Laranjo
authors: R
authors: R
authors: Rodrigues
authors: L
authors: L
authors: Alho
authors: S
authors: S
authors: Oliveira
mesh_terms: D014644:Genetic Variation; D010088:Oxidoreductases / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D011174:Portugal; D012231:Rhizobium / Q000235:genetics / Q000502:physiology*; D019685:Rosales / Q000235:genetics / Q000502:physiology*; D013559:Symbiosis / Q000502:physiology*
chemical_list: D010088:Oxidoreductases; C029130:nitrogenase reductase