Identification of altered gene expression in skeletal muscles from Duchenne muscular dystrophy patients.
Mutations in the dystrophin gene lead to dystrophin deficiency, which is the cause of Duchenne muscular dystrophy (DMD). This important discovery more than 10 years ago opened a new field for very productive investigations. However, the exact functions of dystrophin are still not fully understood and the complex process leading to subsequent muscle fiber necrosis has not been clearly described; hence there has not yet been any marked improvement in patient treatment. To decipher the molecular mechanisms induced by a lack of dystrophin, we started identifying genes whose expression is altered in DMD skeletal muscles. The approach was based on differential screening of a human muscle cDNA array. Nine genes were found to be up- or downregulated. Our results indicate expression alterations in mitochondrial genes, titin, a muscle transcription factor and three novel genes. First characterizations of these novel genes indicated that two of them have striated muscle tissue specificity.
Tkatchenko
A V
AV
INSERM U 300, Faculté de Pharmacie, 15 avenue Charles Flahault, 34060 cedex 01, Montpellier, France.
Piétu
G
G
Cros
N
N
Gannoun-Zaki
L
L
Auffray
C
C
Léger
J J
JJ
Dechesne
C A
CA
D000293:Adolescent; D000595:Amino Acid Sequence; D002648:Child; D064211:Connectin; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D004272:DNA, Mitochondrial / Q000235:genetics; D016189:Dystrophin / Q000172:deficiency* / Q000235:genetics*; D005786:Gene Expression Regulation / Q000235:genetics*; D005809:Genes, Regulator / Q000235:genetics*; D006801:Humans; D008297:Male; D008840:Microfilament Proteins; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000235:genetics*; D018482:Muscle, Skeletal / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D020388:Muscular Dystrophy, Duchenne / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology; D020411:Oligonucleotide Array Sequence Analysis; D011494:Protein Kinases / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D015854:Up-Regulation / Q000235:genetics
C430868:ANKRD46 protein, human; C430866:CMYA5 protein, human; D064211:Connectin; D018076:DNA, Complementary; D004272:DNA, Mitochondrial; D016189:Dystrophin; D008840:Microfilament Proteins; D009124:Muscle Proteins; D012333:RNA, Messenger; C503979:SYNPO2 protein, human; C577285:TTN protein, human; D011494:Protein Kinases