Influence of iron on growth, production of siderophore compounds, membrane proteins, and lipase activity in Acinetobacter calcoaceticus BD 413.
Acinetobacter calcoaceticus BD413 was examined for production of siderophores and iron-repressible outer membrane proteins following growth in iron-restricted media. The iron-scavenging phenotype was associated with the secretion of iron-repressible catechol and the induction of a group of six outer membrane proteins with molecular weights ranging from 34 to 85 kDa. The amount of catechol produced was dependent on medium composition and iron stringency. The relation between iron limitation and lipase production was studied at the level of lipA transcription and extracellular lipase activity. In minimal medium, iron limitation slightly affected lipA expression but decreased exo-lipase activity significantly. However, if iron limitation and rich nitrogen sources were simultaneously present in the culture media, the production of lipase was increased approximately 4 times.
Nudel
C
C
Department of Industrial Microbiology and Biotechnology, Faculty of Pharmacy and Biochemistry, University of Buenos Aires, Argentina. cnudel@ffyb.uba.ar
Gonzalez
R
R
Castañeda
N
N
Mahler
G
G
Actis
L A
LA
D016954:Acinetobacter calcoaceticus / Q000187:drug effects* / Q000254:growth & development / Q000502:physiology; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis / Q000096:biosynthesis*; D002396:Catechols / Q000378:metabolism; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D005786:Gene Expression Regulation / Q000187:drug effects; D007501:Iron / Q000494:pharmacology*; D008049:Lipase / Q000378:metabolism; D008969:Molecular Sequence Data; D017262:Siderophores / Q000032:analysis / Q000096:biosynthesis*
D001425:Bacterial Outer Membrane Proteins; D002396:Catechols; D003470:Culture Media; D017262:Siderophores; D007501:Iron; D008049:Lipase; C034221:catechol