title: Differential expression of integrin alpha v and beta 3 in serosal tissue of human intraperitoneal organs and adhesion.
abstract: OBJECTIVE
  To assess the expression of integrin alpha v and beta 3 in the serosal tissue of intraperitoneal organs and adhesions in persons with and without adhesions.
  
  
  METHODS
  Prospective study.
  
  
  METHODS
  Academic research centers.
  
  
  METHODS
  Fifty-seven patients undergoing abdominal or pelvic surgery.
  
  
  METHODS
  Integrin alpha v and beta 3 messenger RNA (mRNA) expression was evaluated by quantitative reverse transcription polymerase chain reaction.
  
  
  RESULTS
  The serosal tissue of the parietal peritoneum, uterus, fallopian tubes, ovary, and the large and small bowel, as well as peritoneal adhesions, skin, fascia, subcutaneous tissue, and omentum, expresses integrin alpha v and beta 3 mRNA. The level of alpha v and beta 3 mRNA expression varied among these tissues; expression of the former substance was highest in uterine serosa and lowest in the skin and small bowel, and expression of the latter substance was highest in the fallopian tubes and skin and lowest in the uterine serosa. Parietal peritoneum and adhesions express equal levels of integrin alpha v; however, integrin beta 3 expression was >100-fold lower in adhesions than in peritoneum. The level of integrin beta 3 expression in omentum, small and large bowels, and subcutaneous tissue was 100-fold to 10,000-fold lower than in other tissues.
  
  
  CONCLUSIONS
  Serosal tissue of peritoneal organs and adhesions express variable levels of integrin alpha v and beta 3 mRNA. On the basis of such variation and the knowledge that tissue injury alters local integrin expression, integrins may play a key role in adhesion development, particularly in tissue with higher integrin expression.
authors: Department of Obstetrics and Gynecology, University of Florida College of Medicine, Gainesville, Florida 32610-0294, USA. cheginin@obgyn.ufl.edu
authors: N
authors: N
authors: Chegini
authors: K
authors: K
authors: Kotseos
authors: C
authors: C
authors: Ma
authors: R S
authors: RS
authors: Williams
authors: M P
authors: MP
authors: Diamond
authors: L
authors: L
authors: Holmdahl
authors: K
authors: K
authors: Skinner
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015703:Antigens, CD / Q000235:genetics*; D005187:Fallopian Tubes / Q000276:immunology*; D005260:Female; D006801:Humans; D039564:Integrin alphaV; D039661:Integrin beta3; D007421:Intestine, Small / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D010537:Peritoneum / Q000276:immunology*; D010980:Platelet Membrane Glycoproteins / Q000235:genetics*; D017698:Postmenopause; D017697:Premenopause; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012867:Skin / Q000276:immunology; D000267:Tissue Adhesions / Q000276:immunology*; D014158:Transcription, Genetic; D014599:Uterus / Q000276:immunology*
chemical_list: D015703:Antigens, CD; D039564:Integrin alphaV; D039661:Integrin beta3; D010980:Platelet Membrane Glycoproteins; D012333:RNA, Messenger