Operational characteristics of an antibody-immobilized QCM system detecting Salmonella spp.
A quartz crystal microbalance (QCM) system detecting Salmonella spp. was developed by an anti-Salmonella antibody immobilization onto one gold surface of a piezoelectric quartz crystal surface with sulfosuccinimidyl 6-[3-(2-pyridyldithio)propionamido]hexanoate (sulfo-LC-SPDP) thiolation. The optimum temperature and pH for the antibody-immobilized sensor were 35 degrees C and 7.2, respectively. The frequency shifts obtained were correlated with the Salmonella concentrations in the range 3.2 x 10(6)-4.8 x 10(8) CFU per ml. The system was quite specific to Salmonella spp. and applicable for repetitive use after a regeneration step employing 1.2 M NaOH. A model sample measurement was done for a market milk spiked with Salmonella typhimurium.
Park
I S
IS
Korea Food Research Institute, Kyungki-do, Republic of Korea.
Kim
W Y
WY
Kim
N
N
D000818:Animals; D000907:Antibodies, Bacterial; D015374:Biosensing Techniques / Q000379:methods* / Q000706:statistics & numerical data; D004563:Electrochemistry; D008892:Milk / Q000382:microbiology; D011791:Quartz; D012475:Salmonella / Q000276:immunology / Q000302:isolation & purification*; D012486:Salmonella typhimurium / Q000276:immunology / Q000302:isolation & purification; D012680:Sensitivity and Specificity
D000907:Antibodies, Bacterial; D011791:Quartz