Establishment and characterization of an oral melanoma cell line (ME).
A new cell line, ME, has been established from a melanoma of the palatal mucosa. The cultured monolayer of cells was fusiform and melanin-producing. The cells were highly tumorigenic and metastatic in nude mice. The xenographic tumors resembled the original tumor in morphology, melanin production, and the expression of S-100 and HMB-45 antigens. The metaphase karyotype of ME indicated multiple aberrations of chromosomes 2, 3, 5, 7-11, 13, 19, 21 and X. A homozygous loss of the p16/MTS1 gene during the establishment of ME correlated with karyotypic evidence of chromosome 9 abnormalities. Absence of nm23 protein expression and elevated expression of CD44 protein (indicative of metastatic phenotypes) were demonstrated in primary and xenographic tumors. ME cells could be valuable in developing novel therapeutic strategies for oral melanoma.
Chang
K W
KW
Institute of Oral Biology and Faculty of Dentistry National Yang-Ming University, 112, Taipei, Taiwan.
Lin
S C
SC
Chao
S Y
SY
Kwan
P C
PC
Chiu
C P
CP
Wong
Y K
YK
D000368:Aged; D000818:Animals; D000951:Antigens, Neoplasm; D018929:Cell Culture Techniques; D002869:Chromosome Aberrations; D025063:Chromosome Disorders; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000032:analysis; D003593:Cytoplasm / Q000737:chemistry; D005260:Female; D006801:Humans; D018960:Hyaluronan Receptors / Q000032:analysis; D007150:Immunohistochemistry; D007621:Karyotyping; D008543:Melanins / Q000032:analysis; D008545:Melanoma / Q000737:chemistry / Q000235:genetics / Q000473:pathology*; D058950:Melanoma-Specific Antigens; D051379:Mice; D008819:Mice, Nude; D020559:Monomeric GTP-Binding Proteins / Q000032:analysis; D009062:Mouth Neoplasms / Q000737:chemistry / Q000235:genetics / Q000473:pathology*; D054778:NM23 Nucleoside Diphosphate Kinases; D009363:Neoplasm Proteins / Q000032:analysis; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000473:pathology; D009701:Nucleoside-Diphosphate Kinase; D016133:Polymerase Chain Reaction; D009418:S100 Proteins / Q000032:analysis; D017422:Sequence Analysis, DNA; D014157:Transcription Factors / Q000032:analysis; D014407:Tumor Cells, Cultured / Q000737:chemistry / Q000473:pathology*
D000951:Antigens, Neoplasm; D019941:Cyclin-Dependent Kinase Inhibitor p16; D018960:Hyaluronan Receptors; D008543:Melanins; D058950:Melanoma-Specific Antigens; D054778:NM23 Nucleoside Diphosphate Kinases; D009363:Neoplasm Proteins; D009418:S100 Proteins; D014157:Transcription Factors; C517938:NME1 protein, human; C517939:Nme1 protein, mouse; D009701:Nucleoside-Diphosphate Kinase; D020559:Monomeric GTP-Binding Proteins