Identification of CD4+ T cell epitopes on the fusion (F) and attachment (G) proteins of bovine respiratory syncytial virus (BRSV).
To gain insight into the antigenic structure of the F and G proteins of BRSV, we have mapped CD4+ T cell epitopes on these proteins using synthetic peptides and lymphocytes from vaccinated, naturally infected or experimentally infected calves, in proliferation assays. Bovine CD4+ T cells recognised epitopes that were distributed predominantly within the F1 subunit of the F protein, some of which were adjacent to previously identified B cell epitopes. Bovine CD4+ T cell epitopes within the G protein were mainly located within the cytoplasmic tail. Several immunodominant bovine T cell epitopes within the F protein, that were recognised by calves with different haplotypes, are also recognised by human T cells. Thus, cattle and humans appear to recognise similar T cell epitopes on the F protein. Studies using antibodies to bovine MHC class II and BoLA DR-transfected CHO cells as antigen-presenting cells indicated that immunodominant regions of the F and G proteins contained both DR- and DQ-restricted epitopes. The finding that there was little recognition of the extracellular domain of the G protein by T cells has important implications for vaccine design based on the soluble form of this protein.
Fogg
M H
MH
Institute for Animal Health, Compton, Berkshire, RG20 7NN, Newbury, UK.
Parsons
K R
KR
Thomas
L H
LH
Taylor
G
G
D000818:Animals; D017951:Antigen Presentation; D000956:Antigens, Viral / Q000737:chemistry*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D016466:CHO Cells; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology; D006224:Cricetinae; D000939:Epitopes / Q000737:chemistry; D005802:Genes, MHC Class II; D008213:Lymphocyte Activation; D018357:Respiratory Syncytial Virus Infections / Q000276:immunology / Q000662:veterinary; D018112:Respiratory Syncytial Virus, Bovine / Q000737:chemistry / Q000276:immunology*; D014162:Transfection; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000276:immunology*; D014760:Viral Fusion Proteins / Q000737:chemistry / Q000276:immunology*; D014765:Viral Vaccines / Q000494:pharmacology
D000956:Antigens, Viral; D000939:Epitopes; C066028:G protein, Bovine respiratory syncytial virus; D014759:Viral Envelope Proteins; D014760:Viral Fusion Proteins; D014765:Viral Vaccines