Disruption of imprinted expression of U2afbp-rs/U2af1-rs1 gene in mouse parthenogenetic fetuses.
The present study shows that the U2afbp-rs gene, a paternally expressed imprinted gene, is activated and expressed in a biallelic manner from maternal alleles in parthenogenetic mouse fetuses on day 9.5 of gestation. The mean expression was detected to be 88% (31-134%) of that in control biparental fetuses, using real-time quantitative reverse transcription and polymerase chain reaction. This disrupted expression of the gene was associated with changes in the chromatin structure but not with the methylation pattern in the regulation region. The present results show that parental specific expression of imprinted genes is not always maintained in uniparental embryos. This suggests that both parental genomes are necessary to establish parental specific expression of the U2afbp-rs gene.
Sotomaru
Y
Y
Department of Animal Science, Tokyo University of Agriculture, 1737, Funako, Atsugi-shi, Kanagawa 243-0034, Japan.
Kawase
Y
Y
Ueda
T
T
Obata
Y
Y
Suzuki
H
H
Domeki
I
I
Hatada
I
I
Kono
T
T
D000818:Animals; D018507:Gene Expression Regulation, Developmental; D018392:Genomic Imprinting; D051379:Mice; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; D010312:Parthenogenesis; D011506:Proteins / Q000235:genetics*; D012261:Ribonucleoproteins / Q000235:genetics*; D000072668:Splicing Factor U2AF
D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; D011506:Proteins; D012261:Ribonucleoproteins; D000072668:Splicing Factor U2AF; C000607495:Zrsr1 protein, mouse