title: Nifedipine-activated Ca(2+) permeability in newborn rat cortical collecting duct cells in primary culture.
abstract: To characterize Ca(2+) transport in newborn rat cortical collecting duct (CCD) cells, we used nifedipine, which in adult rat distal tubules inhibits the intracellular Ca(2+) concentration ([Ca(2+)](i)) increase in response to hormonal activation. We found that the dihydropyridine (DHP) nifedipine (20 microM) produced an increase in [Ca(2+)](i) from 87.6 +/- 3.3 nM to 389.9 +/- 29.0 nM in 65% of the cells. Similar effects of other DHP (BAY K 8644, isradipine) were also observed. Conversely, DHPs did not induce any increase in [Ca(2+)](i) in cells obtained from proximal convoluted tubule. In CCD cells, neither verapamil nor diltiazem induced any rise in [Ca(2+)](i). Experiments in the presence of EGTA showed that external Ca(2+) was required for the nifedipine effect, while lanthanum (20 microM), gadolinium (100 microM), and diltiazem (20 microM) inhibited the effect. Experiments done in the presence of valinomycin resulted in the same nifedipine effect, showing that K(+) channels were not involved in the nifedipine-induced [Ca(2+)](i) rise. H(2)O(2) also triggered [Ca(2+)](i) rise. However, nifedipine-induced [Ca(2+)](i) increase was not affected by protamine. In conclusion, the present results indicate that 1) primary cultures of cells from terminal nephron of newborn rats are a useful tool for investigating Ca(2+) transport mechanisms during growth, and 2) newborn rat CCD cells in primary culture exhibit a new apical nifedipine-activated Ca(2+) channel of capacitive type (either transient receptor potential or leak channel).
authors: Departamento de Fisiología, Centro de Investigación y de Estudios Avanzados del Institúto Politécnico Nacional, Mexico City DF 07000, Mexico.
authors: L
authors: L
authors: Valencia
authors: M
authors: M
authors: Bidet
authors: S
authors: S
authors: Martial
authors: E
authors: E
authors: Sanchez
authors: E
authors: E
authors: Melendez
authors: M
authors: M
authors: Tauc
authors: C
authors: C
authors: Poujeol
authors: D
authors: D
authors: Martin
authors: M D
authors: MD
authors: Namorado
authors: J L
authors: JL
authors: Reyes
authors: P
authors: P
authors: Poujeol
mesh_terms: D001498:3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester / Q000494:pharmacology; D000818:Animals; D000831:Animals, Newborn; D001692:Biological Transport / Q000187:drug effects; D002118:Calcium / Q000378:metabolism*; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D002463:Cell Membrane Permeability / Q000187:drug effects* / Q000502:physiology; D002478:Cells, Cultured; D003600:Cytosol / Q000378:metabolism; D004095:Dihydropyridines / Q000494:pharmacology; D004110:Diltiazem / Q000494:pharmacology; D004533:Egtazic Acid / Q000494:pharmacology; D005682:Gadolinium / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000494:pharmacology; D017275:Isradipine / Q000494:pharmacology; D007672:Kidney Cortex / Q000166:cytology / Q000502:physiology*; D007685:Kidney Tubules, Collecting / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D007700:Kinetics; D007811:Lanthanum / Q000494:pharmacology; D009543:Nifedipine / Q000494:pharmacology*; D011479:Protamines / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D019284:Thapsigargin / Q000494:pharmacology; D014700:Verapamil / Q000494:pharmacology
chemical_list: D002121:Calcium Channel Blockers; D004095:Dihydropyridines; D011479:Protamines; C028521:lanthanum chloride; D004533:Egtazic Acid; D019284:Thapsigargin; D007811:Lanthanum; D001498:3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester; C038806:1,4-dihydropyridine; D005682:Gadolinium; D006861:Hydrogen Peroxide; D014700:Verapamil; D004110:Diltiazem; D009543:Nifedipine; C038958:gadolinium chloride; D002118:Calcium; D017275:Isradipine