title: Interleukin-1beta in antigen-induced arthritis of the rabbit temporomandibular joint.
abstract: The aim was to investigate joint perfusate levels of interleukin-1beta (IL-1beta) in antigen-induced monoarthritis of the rabbit temporomandibular (TMJ) and knee joints. Twenty-four adult male New Zealand White rabbits were divided into three groups: a control group as well as TMJ arthritis and knee joint arthritis groups. After sensitization, unilateral arthritis was induced by intra-articular injection with ovalbumin and the contralateral joint was injected with saline 3 weeks after induction of arthritis. Joints were then perfused continuously with saline and samples were collected at 10-min intervals over a 50-min period. The IL-1beta concentrations in the samples were then analyzed. After killing the animals, the joints were examined histologically. The IL-1beta concentrations in the samples from the arthritic TMJs and knee joints were significantly higher than in the saline-injected and the control joints. Histological signs of chronic arthritis of similar severity were found in both joints. The IL-1beta levels in the samples from the arthritic TM and knee joints correlated with the histological severity of the arthritis, including pannus formation. In conclusion, this study shows that IL-1beta is released in the synovium of rabbit TMJs and knee joints during antigen-induced arthritis, and that high IL-1beta levels in synovial fluid are associated with histological signs of inflammation including, pannus tissue formation.
authors: First Department of Oral and Maxillofacial Surgery, Kyushu Dental College, 2-6-1 Manazuru, 803-8580, Kitakyushu, Japan. tominaga@kyu-dent.ac.jp
authors: K
authors: K
authors: Tominaga
authors: P
authors: P
authors: Alstergren
authors: H
authors: H
authors: Kurita
authors: A
authors: A
authors: Matsukawa
authors: J
authors: J
authors: Fukuda
authors: S
authors: S
authors: Kopp
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000818:Animals; D001169:Arthritis, Experimental / Q000145:classification / Q000276:immunology* / Q000473:pathology; D007114:Immunization; D007270:Injections, Intra-Articular; D007375:Interleukin-1 / Q000032:analysis*; D007719:Knee Joint / Q000276:immunology / Q000473:pathology; D008297:Male; D010047:Ovalbumin / Q000008:administration & dosage; D011817:Rabbits; D012965:Sodium Chloride; D018709:Statistics, Nonparametric; D013582:Synovial Fluid / Q000276:immunology; D013705:Temporomandibular Joint Disorders / Q000276:immunology* / Q000473:pathology
chemical_list: D000276:Adjuvants, Immunologic; D007375:Interleukin-1; D012965:Sodium Chloride; D010047:Ovalbumin