title: [Application of chromosome painting technique to analysis of structural aberration of human chromosomes].
abstract: OBJECTIVE
  This study was aimed at using chromosome painting technique to detect translocation, especially microtranslocations, on chromosomes in comparison with G-banding analysis.
  
  
  METHODS
  Chromosome painting technique was applied to analysis of metaphase chromosomes of patients for detecting translocations with biotin-labeled chromosomes X, Y, 14q, 10 specific probes.
  
  
  RESULTS
  Fluorescence in situ hybridization FISH signals were shown clearly in slides even in specimen stored at room temperature for 10 years and at -80 centigrade degree. Translocations were located precisely.
  
  
  CONCLUSIONS
  Microtranslocations, which are hard to analyze by G-banding, can be detected exactly using chromosome painting technique with G-band karyotype on metaphase chromosome.
authors: Department of Medical Genetics, Harbin Medical University, Harbin, Heilongjiang 150086 P. R. China. yanghj@ems.hrbmu.edu.cn
authors: H
authors: H
authors: Yang
authors: Y
authors: Y
authors: Song
authors: Q
authors: Q
authors: Liu
authors: Y
authors: Y
authors: Li
authors: S
authors: S
authors: Fu
authors: G
authors: G
authors: Zhang
authors: P
authors: P
authors: Li
mesh_terms: D000328:Adult; D002869:Chromosome Aberrations; D020223:Chromosome Painting; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D014178:Translocation, Genetic