Use of bacterially expressed EBNA-1 protein cloned from a nasopharyngeal carcinoma (NPC) biopsy as a screening test for NPC patients.
EBV serological tests have been used for many years as accessory diagnostic predictors of nasopharyngeal carcinoma (NPC). To increase the sensitivity and specificity of the NPC detection rate, a novel enzyme-linked immunosorbent assay (ELISA) was established using a bacterially-expressed GST-EBNA-1 protein, containing the EBNA-1 sequence cloned from an NPC patient. Serum samples were collected from age- and gender-matched patients with NPC, community control subjects and hospital control patients and tested using this ELISA. The positivity rates were 78.7% (247/314) in NPC, 11.5% (28/244) in hospital controls and 3.8% (10/263) in the community control group. These serum samples were also tested for IgA anti-VCA antibodies and their ability to neutralize EBV DNase and the sensitivities of the anti-VCA antibody and DNase-neutralization tests also were analyzed. The optimum combination is VCA plus EBNA-1, which can identify 92.5% (287/310) of NPC patients, and shows a specificity of 92.7% (242/261) for normal individuals.
Chen
M R
MR
Graduate Institute of Microbiology, College of Medicine, National Taiwan University, Taipei, Taiwan.
Liu
M Y
MY
Hsu
S M
SM
Fong
C C
CC
Chen
C J
CJ
Chen
I H
IH
Hsu
M M
MM
Yang
C S
CS
Chen
J Y
JY
D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral / Q000276:immunology; D001706:Biopsy; D036022:Capsid Proteins; D003001:Cloning, Molecular; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D019309:Epstein-Barr Virus Nuclear Antigens / Q000276:immunology*; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood; D016555:Matched-Pair Analysis; D009303:Nasopharyngeal Neoplasms / Q000175:diagnosis* / Q000821:virology; D009500:Neutralization Tests; D010957:Plasmids; D011993:Recombinant Fusion Proteins / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D012698:Serologic Tests; D013624:Taiwan
D000914:Antibodies, Viral; D000956:Antigens, Viral; D036022:Capsid Proteins; D000939:Epitopes; D019309:Epstein-Barr Virus Nuclear Antigens; C041788:Epstein-Barr viral capsid antigen; D007070:Immunoglobulin A; D011993:Recombinant Fusion Proteins; C417029:EBV-encoded nuclear antigen 1