Transport of lactate and pyruvate in the intraerythrocytic malaria parasite, Plasmodium falciparum.
The mature, intraerythrocytic form of the human malaria parasite, Plasmodium falciparum, is reliant on glycolysis for its energetic requirements. It produces large quantities of lactic acid, which have to be removed from the parasite's cytosol to maintain the cell's integrity and metabolic viability. Here we show that the monocarboxylates lactate and pyruvate are both transported across the parasite's plasma membrane via a H(+)/monocarboxylate symport process that is saturable and inhibited by the bioflavonoid phloretin. The results provide direct evidence for the presence at the parasite surface of a H(+)-coupled monocarboxylate transporter with features in common with members of the MCT (monocarboxylate transporter) family of higher eukaryotes.
Elliott
J L
JL
School of Biochemistry and Molecular Biology, Faculty of Science, Australian National University, Canberra, A.C.T. 0200, Australia.
Saliba
K J
KJ
Kirk
K
K
D000818:Animals; D027321:Anion Transport Proteins; D001692:Biological Transport / Q000187:drug effects / Q000502:physiology; D002352:Carrier Proteins / Q000737:chemistry / Q000378:metabolism / Q000502:physiology; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism / Q000469:parasitology; D006019:Glycolysis; D006801:Humans; D006859:Hydrogen / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D017136:Ion Transport / Q000502:physiology; D007700:Kinetics; D019344:Lactic Acid / Q000378:metabolism*; D016778:Malaria, Falciparum / Q000469:parasitology; D027501:Monocarboxylic Acid Transporters; D010693:Phloretin / Q000494:pharmacology; D010963:Plasmodium falciparum / Q000187:drug effects / Q000378:metabolism*; D019289:Pyruvic Acid / Q000378:metabolism*; D012503:Saponins / Q000494:pharmacology
D027321:Anion Transport Proteins; D002352:Carrier Proteins; D027501:Monocarboxylic Acid Transporters; D012503:Saponins; D019344:Lactic Acid; D006859:Hydrogen; D019289:Pyruvic Acid; D010693:Phloretin