Detection of mRNA for the terminal complement components C5, C6, C8 and C9 in human umbilical vein endothelial cells in vitro.
Human umbilical vein endothelial cells (HUVEC) have previously been shown to synthesize the functional terminal pathway of complement based on the detection by radioimmunoassay of the terminal complement complex (TCC) on coincubated agarose beads. In addition, C7 secretion by these cells in amounts comparable to C3, as well as C7 mRNA, has recently been demonstrated. However, it has not been possible to detect C5-6 and C8 in the fluid phase, and only trace amounts of soluble C9. Against this background we examined whether mRNA for the remaining terminal complement factors was present in HUVEC. By the use of reverse transcription (RT)-polymerase chain reaction (PCR) and Northern blot the presence of mRNA for complement factors C5, C6, C8 and C9 was demonstrated.
Langeggen
H
H
Department of Gastroenterological Surgery, Ullevål University Hospital, Oslo, Norway.
Berge
K E
KE
Macor
P
P
Fischetti
F
F
Tedesco
F
F
Hetland
G
G
Berg
K
K
Johnson
E
E
D001483:Base Sequence; D015152:Blotting, Northern; D002478:Cells, Cultured; D003182:Complement C5 / Q000235:genetics; D003183:Complement C6 / Q000235:genetics; D003185:Complement C8 / Q000235:genetics; D003186:Complement C9 / Q000235:genetics; D003165:Complement System Proteins / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D004730:Endothelium, Vascular / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014471:Umbilical Veins / Q000276:immunology / Q000378:metabolism
D003182:Complement C5; D003183:Complement C6; D003185:Complement C8; D003186:Complement C9; D017931:DNA Primers; D012333:RNA, Messenger; D003165:Complement System Proteins