Investigation into the effects of amyloid (1-42) beta-peptide upon basal and antigen-stimulated hexosaminidase and serotonin release from rat RBL-2H3 basophilic leukemia cells.
There is evidence that beta-amyloid (A beta) peptide infusion in vivo produces a degranulation of vascular mast cells. It would be useful to investigate the interaction between A beta and mast cells in a simple in vitro model system in order to determine the cellular mechanism by which exposure to A beta peptides results in mast cell degranulation. In the present study, the effect of A beta(1-42) upon the release of granular hexosaminidase and serotonin has been investigated using the cognate rat mast cell line, RBL-2H3. Sensitization of these cells for 1 h with anti-DNP IgE (monoclonal anti-dinitrophenyl) results in a large release of hexosaminidase and serotonin due to degranulation when the cells are exposed to DNP-HSA (albumin, human dinitrophenyl). Pretreatment overnight with A beta(1-42) (10 and 30 microM) did not affect either the basal or antigen-stimulated release of hexosaminidase or serotonin. A similar lack of effect of A beta(25-35) and the lipid peroxidation product HNE upon antigen-stimulated release of hexosaminidase or serotonin was also found. It is concluded that RBL-2H3 cells are not a useful model for mechanistic studies into the effects of beta-amyloid peptides upon vascular mast cells.
Englund
P
P
Department of Pharmacology and Clinical Neuroscience, Umeå University, Umeå, Sweden.
Jacobsson
S O
SO
Fowler
C J
CJ
D016229:Amyloid beta-Peptides / Q000494:pharmacology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D000941:Antigens / Q000494:pharmacology*; D015550:Cell Degranulation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D015471:Leukemia, Basophilic, Acute / Q000378:metabolism*; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism; D010446:Peptide Fragments / Q000494:pharmacology*; D051381:Rats; D012701:Serotonin / Q000378:metabolism*; D012709:Serum Albumin / Q000276:immunology; D014407:Tumor Cells, Cultured; D001619:beta-N-Acetylhexosaminidases / Q000378:metabolism*
D016229:Amyloid beta-Peptides; D000911:Antibodies, Monoclonal; D000941:Antigens; D010446:Peptide Fragments; D012709:Serum Albumin; C075222:amyloid beta-protein (1-42); D012701:Serotonin; D001619:beta-N-Acetylhexosaminidases