Rapid and sensitive biosensor for Salmonella.
The rapid and sensitive detection of Salmonella typhymurium based on the use of a polyclonal antibody immobilized by the Langmuir-Blodgett method on the surface of a quartz crystal acoustic wave device was demonstrated. The binding of bacteria to the surface changed the crystal resonance parameters; these were quantified by the output voltage of the sensor instrumentation. The sensor had a lower detection limit of a few hundred cells/ml, and a response time of < 100 s over the range of 10(2)-10(10) cells/ml. The sensor response was linear between bacterial concentrations of 10(2)-10(7) cells/ml, with a sensitivity of 18 mV/decade. The binding of bacteria was specific with two binding sites needed to bind a single cell. The sensors preserve approximately 75% of their sensitivity over a period of 32 days.
Pathirana
S T
ST
Institute for Biological Detection Systems, Department of Anatomy, Physiology and Pharmacology, Auburn University, Alabama, 36849, USA.
Barbaree
J
J
Chin
B A
BA
Hartell
M G
MG
Neely
W C
WC
Vodyanoy
V
V
D000818:Animals; D000907:Antibodies, Bacterial; D001431:Bacteriological Techniques; D015374:Biosensing Techniques / Q000379:methods* / Q000706:statistics & numerical data; D005516:Food Microbiology; D006801:Humans; D012486:Salmonella typhimurium / Q000276:immunology / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D013997:Time Factors
D000907:Antibodies, Bacterial