title: Mutations affecting nerve attachment of Caenorhabditis elegans.
abstract: Using a pan-neuronal GFP marker, a morphological screen was performed to detect Caenorhabditis elegans larval lethal mutants with severely disorganized major nerve cords. We recovered and characterized 21 mutants that displayed displacement or detachment of the ventral nerve cord from the body wall (Ven: ventral cord abnormal). Six mutations defined three novel genetic loci: ven-1, ven-2, and ven-3. Fifteen mutations proved to be alleles of previously identified muscle attachment/positioning genes, mup-4, mua-1, mua-5, and mua-6. All the mutants also displayed muscle attachment/positioning defects characteristic of mua/mup mutants. The pan-neuronal GFP marker also revealed that mutants of other mua/mup loci, such as mup-1, mup-2, and mua-2, exhibited the Ven defect. The hypodermis, the excretory canal, and the gonad were morphologically abnormal in some of the mutants. The pleiotropic nature of the defects indicates that ven and mua/mup genes are required generally for the maintenance of attachment of tissues to the body wall in C. elegans.
authors: Division of Biological Science, Nagoya University Graduate School of Science, Chikusa-ku, Nagoya 464-8602, Japan.
authors: G
authors: G
authors: Shioi
authors: M
authors: M
authors: Shoji
authors: M
authors: M
authors: Nakamura
authors: T
authors: T
authors: Ishihara
authors: I
authors: I
authors: Katsura
authors: H
authors: H
authors: Fujisawa
authors: S
authors: S
authors: Takagi
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics*; D002874:Chromosome Mapping; D017238:Genes, Helminth; D007814:Larva; D009132:Muscles / Q000294:innervation*; D009154:Mutation; D010641:Phenotype