Lectin cytochemistry of rat cerebral and cerebellar neuronal cells.
Lectin cytochemistry of normal rat central nervous system (CNS) was performed on formalin-fixed, paraffin-embedded sections by using 14 lectins and light and electron microscopy. Hippocampal neurons reacted positively with Griffonia (Bandeiraea) simplicifolia lectin I (GSL-I), Griffonia (Bandeiraea) simplicifolia lectin II (GSL-II) and Vicia villosa agglutinin (VVA); neurons in the cornu ammonis (CA) 2 region reacted positively with GSL-I and GSL-II, and neurons in the CA2 and CA3 regions reacted with VVA. Cortical neurons reacted positively with GSL-I and VVA. Cerebellar Purkinje cells and granule cells were reactive with Phaseolus vulgaris leukoagglutinin (PHA-L). Cerebellar nucleus neurons were also reactive with PHA-L and VVA. Reactivity with GSL-I, GSL-II, VVA and PHA-L was observed only on the neuronal cell surface. The present findings demonstrate the differences between cerebral and cerebellar neurons in reacting with glycoconjugates.
Kawai
K
K
Department of Neuropsychiatry, Okayama University Medical School, Japan.
Ishizu
H
H
Kuroda
S
S
D000818:Animals; D002531:Cerebellum / Q000737:chemistry* / Q000166:cytology; D002540:Cerebral Cortex / Q000737:chemistry* / Q000166:cytology; D002831:Choroid Plexus / Q000737:chemistry / Q000166:cytology; D006651:Histocytochemistry; D037102:Lectins; D008854:Microscopy, Electron; D010835:Phytohemagglutinins; D037121:Plant Lectins; D011689:Purkinje Cells / Q000737:chemistry* / Q000648:ultrastructure; D017966:Pyramidal Cells / Q000737:chemistry* / Q000648:ultrastructure; D051381:Rats; D017208:Rats, Wistar
C045475:Griffonia simplicifolia lectins; D037102:Lectins; D010835:Phytohemagglutinins; D037121:Plant Lectins; C042242:Vicia lectins; C100156:leukoagglutinins, plants