Binding of a porphyrin conjugate of Hoechst 33258 to DNA. II. NMR spectroscopic studies detect multiple binding modes to a 12-mer nonself-complementary duplex DNA.
We have probed by 1H NMR spectroscopy the molecular basis of the interaction between Hoechst 33258 conjugated to a des-metalloporphyrin and a non self-complementary duplex DNA sequence, designed on the known chemical nuclease selectivity of this system. The imino NMR spectra are consistent with two distinct families of structure, that is, PORHOE binding either way along the duplex. 2D spectral, T2, and linewidth data suggest multiple species within the two conformational families.
Frau
S
S
School of Pharmacy and Pharmaceutical Sciences, University of Manchester, UK.
Bichenkova
E V
EV
Morris
G A
GA
Douglas
K T
KT
D001665:Binding Sites; D006690:Bisbenzimidazole / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D018076:DNA, Complementary / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D008024:Ligands; D009682:Magnetic Resonance Spectroscopy; D009841:Oligonucleotides / Q000737:chemistry; D011166:Porphyrins / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
D018076:DNA, Complementary; D005456:Fluorescent Dyes; D008024:Ligands; D009841:Oligonucleotides; D011166:Porphyrins; D004247:DNA; D006690:Bisbenzimidazole