Amyloid and nonfibrillar deposits in mice transgenic for wild-type human transthyretin: a possible model for senile systemic amyloidosis.
The human serum protein transthyretin (TTR) is highly fibrillogenic in vitro and is the fibril precursor in both autosomal dominant (familial amyloidotic polyneuropathy [FAP] and familial amyloidotic cardiomyopathy [FAC]) and sporadic (senile systemic amyloidosis [SSA]) forms of human cardiac amyloidosis. We have produced mouse strains transgenic for either wild-type or mutant (TTRLeu55Pro) human TTR genes. Eighty-four percent of C57BI/6xDBA/2 mice older than 18 months, transgenic for the wild-type human TTR gene, develop TTR deposits that occur primarily in heart and kidney. In most of the animals, the deposits are nonfibrillar and non-Congophilic, but 20% of animals older than 18 months that bear the transgene have human TTR cardiac amyloid deposits identical to the lesions seen in SSA. Amino terminal amino acid sequence analysis and mass spectrometry of the major component extracted from amyloid and nonamyloid deposits revealed that both were intact human TTR monomers with no evidence of proteolysis or codeposition of murine TTR. This is the first instance in which the proteins from amyloid and nonfibrillar deposits in the same or syngeneic animals have been shown to be identical by sequence analysis. It is also the first time in any form of amyloidosis that nonfibrillar deposits have been shown to systematically occur temporally before the appearance of fibrils derived from the same precursor in the same tissues. These findings suggest, but do not prove, that the nonamyloid deposits represent a precursor of the fibril. The differences in the ultrastructure and binding properties of the deposits, despite the identical sizes and amino terminal amino acid sequences of the TTR and the dissociation of deposition and fibril formation, provide evidence that in vivo factors, perhaps associated with aging, impact on both systemic precursor deposition and amyloid fibril formation.
Teng
M H
MH
Research Service, New York Department of Veterans Affairs Medical Center, New York, USA.
Yin
J Y
JY
Vidal
R
R
Ghiso
J
J
Kumar
A
A
Rabenou
R
R
Shah
A
A
Jacobson
D R
DR
Tagoe
C
C
Gallo
G
G
Buxbaum
J
J
D000375:Aging / Q000473:pathology; D000686:Amyloidosis / Q000235:genetics / Q000473:pathology*; D000818:Animals; D003001:Cloning, Molecular; D004195:Disease Models, Animal; D005260:Female; D015870:Gene Expression; D006801:Humans; D007678:Kidney Glomerulus / Q000737:chemistry / Q000473:pathology / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008822:Mice, Transgenic; D016253:Microscopy, Immunoelectron; D018485:Muscle Fibers, Skeletal / Q000737:chemistry / Q000473:pathology / Q000648:ultrastructure; D009206:Myocardium / Q000737:chemistry / Q000473:pathology; D011228:Prealbumin / Q000032:analysis / Q000235:genetics*; D019076:Transgenes
D011228:Prealbumin