title: Interaction of alpha-agglutinin and a-agglutinin, Saccharomyces cerevisiae sexual cell adhesion molecules.
abstract: alpha-Agglutinin and a-agglutinin are complementary cell adhesion glycoproteins active during mating in the yeast Saccharomyces cerevisiae. They bind with high affinity and high specificity: cells of opposite mating types are irreversibly bound by a few pairs of agglutinins. Equilibrium and surface plasmon resonance kinetic analyses showed that the purified binding region of alpha-agglutinin interacted similarly with purified a-agglutinin and with a-agglutinin expressed on cell surfaces. At 20 degrees C, the K(D) for the interaction was 2 x 10(-9) to 5 x 10(-9) M. This high affinity was a result of a very low dissociation rate ( approximately 2.6 x 10(-4) s(-1)) coupled with a low association rate (= 5 x 10(4) M(-1) s(-1)). Circular-dichroism spectroscopy showed that binding of the proteins was accompanied by measurable changes in secondary structure. Furthermore, when binding was assessed at 10 degrees C, the association kinetics were sigmoidal, with a very low initial rate. An induced-fit model of binding with substantial apposition of hydrophobic surfaces on the two ligands can explain the observed affinity, kinetics, and specificity and the conformational effects of the binding reaction.
authors: Department of Biological Sciences and the Institute for Biomolecular Structure and Function, Hunter College of the City University of New York, New York, 10021, USA.
authors: H
authors: H
authors: Zhao
authors: Z M
authors: ZM
authors: Shen
authors: P C
authors: PC
authors: Kahn
authors: P N
authors: PN
authors: Lipke
mesh_terms: D000373:Agglutinins / Q000378:metabolism*; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D005656:Fungal Proteins / Q000378:metabolism* / Q000493:pharmacokinetics; D007700:Kinetics; D000072235:Mating Factor; D010455:Peptides / Q000378:metabolism*; D011485:Protein Binding; D011994:Recombinant Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins; D013696:Temperature
chemical_list: D000373:Agglutinins; D015815:Cell Adhesion Molecules; D005656:Fungal Proteins; D010455:Peptides; D011994:Recombinant Proteins; D029701:Saccharomyces cerevisiae Proteins; C069939:a-agglutinin protein, S cerevisiae; D000072235:Mating Factor