Transformation-associated changes in gene expression of alternative splicing regulatory factors in mouse fibroblast cells.
Although the alternative splicing of various genes is a common phenomenon in tumorigenesis, little is known about the mechanism behind it. Recently, we found altered expression of splicing regulatory factors during two-step chemical transformation in vitro. However, it remains unknown whether such altered expression of splicing factors commonly occur during other modes of transformation. We have further investigated the expression of five splicing regulatory factors, heterogeneous nuclear ribonucleoprotein (hnRNP) A1 and A2, alternative splicing factor/splicing factor 2 (ASF/SF2), spliceosome component of 35-kDa protein (SC35) and 65-kDa subunit of U2 snRNP auxiliary factor (U2AF65), using two series of clonally-derived mouse fibroblast cell lines, that were SV40 large T transfectants (SV-T2 and NIH3T3/13C7) or c-erbB2 transfectants (A4). U2AF65 expression was increased (2.1- and 2.7-fold) in NIH3T3/13C7 and A4 compared with the normal parental cells, respectively, and SC35 expression was increased 1.8- to 2.3-fold in all transformed cells. These results suggest that altered expression of some splicing regulatory factors may commonly occur during various modes of cellular transformation.
Maeda
T
T
Department of Radiology and Radiation Oncology, Osaka University Graduate School of Dentistry, Osaka, Japan. tmaeda@radiol.dent.osaka-u.ac.jp
Furukawa
S
S
D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000494:pharmacology; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D017931:DNA Primers; D005347:Fibroblasts / Q000378:metabolism*; D015870:Gene Expression; D000076245:Heterogeneous Nuclear Ribonucleoprotein A1; D034481:Heterogeneous-Nuclear Ribonucleoprotein Group A-B; D034441:Heterogeneous-Nuclear Ribonucleoproteins; D051379:Mice; D008807:Mice, Inbred BALB C; D009687:Nuclear Proteins / Q000235:genetics*; D012326:RNA Splicing / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis; D016601:RNA-Binding Proteins; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000378:metabolism; D012261:Ribonucleoproteins / Q000235:genetics*; D000068103:Serine-Arginine Splicing Factors; D000072668:Splicing Factor U2AF
D000952:Antigens, Polyomavirus Transforming; D017931:DNA Primers; D000076245:Heterogeneous Nuclear Ribonucleoprotein A1; D034481:Heterogeneous-Nuclear Ribonucleoprotein Group A-B; D034441:Heterogeneous-Nuclear Ribonucleoproteins; D009687:Nuclear Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D018719:Erb-b2 Receptor Tyrosine Kinases; D012261:Ribonucleoproteins; D000068103:Serine-Arginine Splicing Factors; D000072668:Splicing Factor U2AF; C499372:SRSF2 protein, mouse; C000607495:Zrsr1 protein, mouse; C439768:hnRNP A2