Chromosomal location of ribosomal DNA (rDNA), (GATA)n and (TTAGGG)n telomeric repeats in the neogastropod Fasciolaria lignaria (Mollusca: Prosobranchia).
This paper reports on a successful application of fluorescent in situ hybridization (FISH) with three repetitive DNA probes (ribosomal DNA (rDNA), (GATA)n and (TTAGGG)n) in the chromosomes of Fasciolaria lignaria (Mollusca: Prosobranchia: Neogastropoda). rDNA FISH consistently identified four chromosome pairs per spread in the three examined specimens. The telomeric sequence (TTAGGG)n hybridized with termini of all chromosomes. GATA FISH revealed abundant, dispersed minisatellite regions which were not associated to the XY sex-determining mechanism as indicated by the absence of a Y specific pattern of labelling.
Vitturi
R
R
Dipartimeinto di Biologia Animale, Università di Palermo, Italy. zuvitcol@unipa.it
Colomba
Gianguzza
P
P
Pirrone
A M
AM
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002875:Chromosomes / Q000235:genetics*; D015342:DNA Probes / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics*; D005260:Female; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D018598:Minisatellite Repeats; D008974:Mollusca / Q000235:genetics*; D019849:Sex Determination Processes; D016615:Telomere / Q000235:genetics
D015342:DNA Probes; D004275:DNA, Ribosomal