title: The severity of cutaneous and oral pemphigus is related to desmoglein 1 and 3 antibody levels.
abstract: BACKGROUND
  Pemphigus vulgaris (PV) and foliaceus (PF) are characterized by antibodies to the desmosomal proteins desmoglein 3 (Dsg3) and desmoglein 1 (Dsg1), respectively. Past studies using indirect immunofluorescence (IIF) as a measure of pemphigus antibody levels have failed to demonstrate consistently a relationship between disease severity and IIF titres. However, IIF is not able to measure separately Dsg1 and 3 antibodies, unlike enzyme-linked immunosorbent assays (ELISA), which utilize recombinant proteins.
  
  
  OBJECTIVE
  To compare independently Dsg1 and 3 antibody levels with the severity of both cutaneous and oral involvement in PV and PF. Patients and methods Four hundred and twenty-four serum samples were analysed from 80 subjects with PV and 24 with PF. IgG antibodies to Dsg1 and 3 were measured by ELISA. For every sample analysed, the associated severity of skin and oral disease were graded from 0 to 3; quiescent, mild, moderate and severe.
  
  
  RESULTS
  A relationship between Dsg1 antibodies and skin severity was demonstrated such that a 10-unit increase in Dsg1 ELISA value was associated with a 34% chance of having a higher severity score [95% confidence interval (CI), 25-45%, P < 0.0005]. This was observed in both PV and PF. Oral severity was associated with Dsg3 antibody levels and a 10-unit increase in the Dsg3 ELISA value was associated with a 25% chance of a higher oral severity score (CI 17-33%, P < 0.0005). We were unable to demonstrate a relationship between Dsg1 antibodies and oral severity, even after adjusting for the effect of Dsg3 antibodies. Similarly, there was no relationship between Dsg3 antibodies and skin severity.
  
  
  CONCLUSIONS
  This study suggests that the clinical phenotype of pemphigus, in particular the balance of skin and oral disease, is determined principally by the quantities of Dsg1 and 3 autoantibodies, respectively.
authors: St John's Institute of Dermatology, St Thomas' Hospital, London, UK. karen.harman@kcl.ac.uk
authors: K E
authors: KE
authors: Harman
authors: P T
authors: PT
authors: Seed
authors: M J
authors: MJ
authors: Gratian
authors: B S
authors: BS
authors: Bhogal
authors: S J
authors: SJ
authors: Challacombe
authors: M M
authors: MM
authors: Black
mesh_terms: D001323:Autoantibodies / Q000097:blood*; D001324:Autoantigens / Q000276:immunology; D015415:Biomarkers / Q000097:blood; D015820:Cadherins / Q000276:immunology*; D051183:Desmoglein 1; D051184:Desmoglein 3; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D009059:Mouth Diseases / Q000276:immunology*; D010392:Pemphigus / Q000276:immunology*; D012720:Severity of Illness Index
chemical_list: D001323:Autoantibodies; D001324:Autoantigens; D015415:Biomarkers; D015820:Cadherins; C071894:DSG3 protein, human; D051183:Desmoglein 1; D051184:Desmoglein 3