Neuroprotective effects in gerbils of spiramine T from Spiraea japonica var. acuta.
The neuroprotective effects of spiramine T, an atisine-type diterpenoid alkaloid isolated from the Chinese herbal medicine Spiraea japonica var. acuta (Rosaceaee), on cerebral ischemia-reperfusion injury produced by 10-min bilateral occlusion of the common carotid arteries followed by 5-day reperfusion in gerbils were investigated. Intravenous spiramine T (0.38, 0.75, and 1.5 mg.kg-1) markedly reduced the stroke index, enhanced the recovery of EEG amplitude during reperfusion and decreased the concentrations of cortex calcium and LPO in a dose-dependent manner. However, no significant effects on water and sodium contents were observed. These results suggested that spiramine T exhibited protective effects on cerebral ischemia-reperfusion injury in gerbils, and its mechanism might be related to reducing calcium accumulation and lipid peroxidation. This is the first report on spiramine T as a natural product with neuroprotective effects.
Li
L
L
Yunnan Pharmacological Laboratories of Natural Products, Kunming Medical College, Kunming, P.R. China.
Nie
J
J
Shen
Z
Z
Wu
W
W
Chen
Z
Z
Hao
X
X
D000818:Animals; D002545:Brain Ischemia / Q000188:drug therapy*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification* / Q000494:pharmacology / Q000627:therapeutic use; D005260:Female; D005849:Gerbillinae; D006576:Heterocyclic Compounds, 4 or More Rings / Q000302:isolation & purification* / Q000494:pharmacology / Q000627:therapeutic use; D008054:Lipid Peroxides / Q000378:metabolism; D008297:Male; D015394:Molecular Structure; D018696:Neuroprotective Agents / Q000302:isolation & purification* / Q000494:pharmacology / Q000627:therapeutic use; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D015427:Reperfusion Injury / Q000188:drug therapy; D012393:Rosacea
D004365:Drugs, Chinese Herbal; D006576:Heterocyclic Compounds, 4 or More Rings; D008054:Lipid Peroxides; D018696:Neuroprotective Agents; C423865:spiramine T