A high-fat meal does not activate blood coagulation factor VII in minipigs.
It is a matter of debate whether postprandial activation of blood coagulation factor VII (FVII) is associated with an increased risk of thrombosis. To clarify this question, an animal model in which consequences of dietary FVII activation can be studied in a more detailed way would be an important tool. We studied postprandial FVII activation in seven non-fasting Göttingen minipigs. Intralipid (4 g/kg) was administered through a gastric tube in two fractions at 9.00 a.m. (one-third of total dose) and 10.30 a.m. (two-thirds of total dose). Blood samples were drawn 0.5 h before (baseline) and 2, 3, 3.5, 4, 5, and 6 h after the first fat load. Triglycerides, activated FVII (FVIIa), FVII coagulant activity (FVIIc), FVII amidolytic activity (FVIIam) and prothrombin fragment I + 2 (F1 + 2) were analysed in plasma samples. Median plasma triglycerides were significantly raised from 0.67 mmol/l (baseline) to 2.56 mmol/l 5 h postprandially (P < 0.001). There were no significant changes in FVIIa (9.6 U/l at baseline), FVIIam (142% at baseline) and F1 + 2 (0.13 nmol/l at baseline). FVIIc decreased from 141% at baseline to 114% 6 h postprandially (P < 0.001). As a high-fat meal does not seem to activate blood coagulation FVII in minipigs, the pig is apparently not a relevant model for the study of dietary FVII activation and thrombin generation.
Olsen
A K
AK
Department of Thrombosis Research, University of Southern Denmark. aako@ribeamt.dk
Larsen
L F
LF
Bladbjerg
E M
EM
Hansen
A K
AK
Jespersen
J
J
Marckmann
P
P
D000818:Animals; D001777:Blood Coagulation; D004041:Dietary Fats / Q000008:administration & dosage*; D005167:Factor VII / Q000378:metabolism*; D015942:Factor VIIa / Q000378:metabolism; D005217:Fat Emulsions, Intravenous / Q000008:administration & dosage; D006801:Humans; D007441:Intubation, Gastrointestinal; D007700:Kinetics; D023421:Models, Animal; D010446:Peptide Fragments / Q000097:blood; D011516:Prothrombin; D013556:Swine, Miniature; D014280:Triglycerides / Q000097:blood
D004041:Dietary Fats; D005217:Fat Emulsions, Intravenous; D010446:Peptide Fragments; D014280:Triglycerides; C045879:prothrombin fragment 1.2; D005167:Factor VII; D011516:Prothrombin; D015942:Factor VIIa