title: Size, shape and secondary structure of calponin.
abstract: The overall size and shape of the chicken gizzard calponin (CaP) h1 molecule was investigated by dynamic light scattering (DLS) measurements. From the DLS experiments, a z-averaged translational diffusion coefficient is derived (5.75 +/- 0.3) x 10(-7) cm(2) s(-1), which corresponds to a hydrodynamic radius of 3.72 nm for calponin. The frictional ratio (1.8 for the unhydrated molecule and 1.5 for the hydrated one) suggests a pronounced anisotropic structure for the molecule. An ellipsoidal model in length 19.4 nm and with a diameter of 2.6 nm used for hydrodynamic calculations was found to reproduce the DLS experimental data. The evaluation of the secondary structure of CaP h1 from the CD spectra by two independent methods has revealed that it contains, on average, 23% helix, 19% beta-strand, 18% beta-turns and loops, and 40% of remainder structures. These values are in good agreement with those predicted from the amino-acid sequence. Predictions used for CaP h1 were applied to other isoforms of known sequences and revealed that all calponins share a common secondary structure. Moreover, the predicted structure of the calponin CH domain is identical to that found by X-ray studies of the spectrin, fimbrin and utrophin CH domains.
authors: Nencki Institute of Experimental Biology, Department of Muscle Biochemistry, Warszawa, Poland. eczurylo@nencki.gov.pl
authors: E A
authors: EA
authors: Czurylo
authors: W
authors: W
authors: Eimer
authors: N
authors: N
authors: Kulikova
authors: T
authors: T
authors: Hellweg
mesh_terms: D020816:Amino Acid Motifs; D000818:Animals; D001665:Binding Sites; D002135:Calcium-Binding Proteins / Q000737:chemistry*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002645:Chickens; D002942:Circular Dichroism; D008027:Light; D008840:Microfilament Proteins; D020033:Protein Isoforms / Q000737:chemistry; D017433:Protein Structure, Secondary; D012542:Scattering, Radiation; D000096985:Calponins
chemical_list: D002135:Calcium-Binding Proteins; D008840:Microfilament Proteins; D020033:Protein Isoforms; D000096985:Calponins