title: Dynamics of the proton transfer reaction on the cytoplasmic surface of bacteriorhodopsin.
abstract: The cytoplasmic surface of bacteriorhodopsin is characterized by a group of carboxylates that function as a proton attractive domain [Checover, S., Nachliel, E., Dencher, N. A., and Gutman, M. (1997) Biochemistry 36, 13919-13928]. To identify these carboxylates, we selectively mutated them into cysteine residues and monitored the effects of the dynamics of proton transfer between the bulk and the surface of the protein. The measurements were carried out without attachment of a pH-sensor to the cysteine residue, thus avoiding any structural perturbation and change in the surface charge caused by the attachment of a reporter group, and the protein was in its BR state. The purple membranes were suspended in an unbuffered solution of pyranine (8-hydroxypyrene-1,3,6-trisulfonate) and exposed to a train of 1000 laser pulses (2.1 mJ/pulse, lambda = 355 nm, at 10 Hz). The excitation of the dye ejected the hydroxyl's proton, and a few nanoseconds later, a pair of free protons and ground-state pyranine anion was formed. The experimental observation was the dynamics of the relaxation of the system to the prepulse state. The observed signals were reconstructed by a numeric method that replicates the chemical reactions proceeding in the perturbed space. The detailed reconstruction of the measured signal assigned the various proton-binding sites with rate constants for proton binding and proton exchange and the pK values. Comparison of the results obtained by the various mutants indicates that the dominant proton-binding cluster of the wild-type protein consists of D104, E161, and E234. The replacement of D104 or E161 with cysteine lowered the proton binding capacity of the cluster to approximately 60% of that of the native protein. The replacement of E234 with cysteine disrupted the structure of the cluster, causing the two remaining carboxylates to function as isolated residues that do not interact with each other. The possibility of proton transfer between monomers is discussed.
authors: Technische Universitat Darmstadt, Institut fur Biochemie, Darmstadt D-64287, Germany.
authors: S
authors: S
authors: Checover
authors: Y
authors: Y
authors: Marantz
authors: E
authors: E
authors: Nachliel
authors: M
authors: M
authors: Gutman
authors: M
authors: M
authors: Pfeiffer
authors: J
authors: J
authors: Tittor
authors: D
authors: D
authors: Oesterhelt
authors: N A
authors: NA
authors: Dencher
mesh_terms: D000838:Anions; D001190:Arylsulfonates / Q000737:chemistry; D001224:Aspartic Acid / Q000737:chemistry / Q000235:genetics; D001436:Bacteriorhodopsins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D001665:Binding Sites / Q000235:genetics; D002021:Buffers; D002264:Carboxylic Acids / Q000737:chemistry; D003593:Cytoplasm / Q000737:chemistry / Q000378:metabolism; D005110:Extracellular Space / Q000737:chemistry / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry; D018698:Glutamic Acid / Q000737:chemistry / Q000235:genetics; D019617:Halobacterium salinarum; D007700:Kinetics; D007834:Lasers; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D010782:Photolysis; D011522:Protons; D055672:Static Electricity; D013499:Surface Properties; D013816:Thermodynamics
chemical_list: D000838:Anions; D001190:Arylsulfonates; D002021:Buffers; D002264:Carboxylic Acids; D005456:Fluorescent Dyes; D010446:Peptide Fragments; D011522:Protons; D001224:Aspartic Acid; D018698:Glutamic Acid; D001436:Bacteriorhodopsins; C005047:pyranine