Enzymatic hydrolysis of waste office paper using viscosity as operating parameter.
Enzymatic hydrolysis of waste office (WO) paper with feeding WO paper in a reactor was investigated using apparent viscosity as operating parameter. Since the apparent viscosity was correlated with the concentration of pulping WO paper, the amount of hydrolyzed WO paper was assumed by measuring the decrease in the apparent viscosity. Then the amount of hydrolysis WO paper and the amount of enzyme corresponding to the desired ratio were fed into the reactor. When the WO paper and 1% (to the amount of WO paper) enzyme were fed to the hydrolytic reaction, 87 g/L of reducing sugar (RS) with a hydrolytic yield of 42.2% was obtained for a 24-h hydrolysis. However, when nonpulping WO paper and 5% (to the amount of WO paper) enzyme were fed to the hydrolytic reaction, 120 g/L of RS with a hydrolytic yield of 40% was obtained for a 24-h hydrolysis. Therefore, the RS concentration from this hydrolysis process feeding WO paper using apparent viscosity as operating parameter may be of sufficient concentration to serve as a carbon source in microorganism culture or chemical feedstock.
Park
E Y
EY
Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, 836 Ohya, Shizuoka 422-8529, Japan. yspark@agr.shizuoka.ac.jp
Michinaka
A
A
Okuda
N
N
D000164:Acremonium / Q000201:enzymology; D002480:Cellulase / Q000378:metabolism*; D002482:Cellulose / Q000378:metabolism*; D006868:Hydrolysis; D007220:Industrial Waste; D010209:Paper; D014783:Viscosity
D007220:Industrial Waste; D002482:Cellulose; D002480:Cellulase