Roles of rpoN, fliA, and flgR in expression of flagella in Campylobacter jejuni.
Three potential regulators of flagellar expression present in the genome sequence of Campylobacter jejuni NCTC 11168, the genes rpoN, flgR, and fliA, which encode the alternative sigma factor sigma(54), the sigma(54)-associated transcriptional activator FlgR, and the flagellar sigma factor sigma(28), respectively, were investigated for their role in global regulation of flagellar expression. The three genes were insertionally inactivated in C. jejuni strains NCTC 11168 and NCTC 11828. Electron microscopic studies of the wild-type and mutant strains showed that the rpoN and flgR mutants were nonflagellate and that the fliA mutant had truncated flagella. Immunoblotting experiments with the three mutants confirmed the roles of rpoN, flgR, and fliA in the expression of flagellin.
Jagannathan
A
A
School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, United Kingdom.
Constantinidou
C
C
Penn
C W
CW
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D016123:Campylobacter jejuni / Q000166:cytology / Q000235:genetics*; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000235:genetics*; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D005407:Flagella / Q000235:genetics* / Q000378:metabolism; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D051922:PII Nitrogen Regulatory Proteins; D051877:RNA Polymerase Sigma 54; D016415:Sequence Alignment; D012808:Sigma Factor / Q000235:genetics*; D015534:Trans-Activators; D014157:Transcription Factors / Q000235:genetics*
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C064971:FliA protein, Bacteria; D051922:PII Nitrogen Regulatory Proteins; D012808:Sigma Factor; D015534:Trans-Activators; D014157:Transcription Factors; D012321:DNA-Directed RNA Polymerases; D051877:RNA Polymerase Sigma 54