Fabrication and evaluation of a carbon-based dual-electrode detector for poly(dimethylsiloxane) electrophoresis chips.
The first carbon-based dual-electrode detector for microchip capillary electrophoresis (CE) is described. The poly(dimethylsiloxane) (PDMS)-based microchip CE devices were constructed by reversibly sealing a PDMS layer containing separation and injection channels to another PDMS layer containing carbon fiber working electrodes. End-channel amperometric detection was employed and the performance of the chip was evaluated using catechol. The response was found to be linear between 1 and 600 microM with an experimentally determined limit of detection (LOD) of 500 nM and a sensitivity of 30 pA/microM. Collection efficiencies for catechol ranged from 36.0 to 43.7% at field strengths of 260-615 V/cm. The selectivity that can be gained with these devices is demonstrated by the first CE-based dual-electrode detection of a Cu(II) peptide complex. These devices illustrate the potential for a rugged and easily constructed microchip CE system with an integrated carbon-based detector of similar scale.
Gawron
A J
AJ
Department of Pharmaceutical Chemistry and Center for Bioanalytical Research, University of Kansas, Lawrence 66047, USA.
Martin
R S
RS
Lunte
S M
SM
D002244:Carbon; D003300:Copper; D004129:Dimethylpolysiloxanes; D004563:Electrochemistry; D019075:Electrophoresis, Capillary / Q000295:instrumentation*; D004867:Equipment Design; D008832:Microchemistry / Q000295:instrumentation*; D008839:Microelectrodes; D008904:Miniaturization; D018377:Neurotransmitter Agents / Q000032:analysis; D010084:Oxidation-Reduction; D015203:Reproducibility of Results; D012828:Silicones
D004129:Dimethylpolysiloxanes; D018377:Neurotransmitter Agents; D012828:Silicones; C013830:baysilon; D002244:Carbon; D003300:Copper