Histogram method to obtain heat capacities in lipid monolayers, curved bilayers, and membranes containing peptides.
Lipid monolayer chain melting transitions were simulated using a two-state Doniach model, and experimental melting profiles of lipid vesicles were analyzed. We sampled the information of a Monte Carlo simulation into a single broad histogram containing complete information about the distribution of states. The information of the monolayer histogram was first used to calculate the melting behavior of a bilayer constructed from two uncoupled monolayers. We then fitted calorimetric heat profiles of various preparations of dipalmitoyl phosphatidylcholine vesicles. This analysis was extended to lipid bilayers. A fixed mean bilayer curvature was shown to result in a broadening of bilayer melting profiles. We furthermore used the histogram method to obtain the chain melting behavior of simple lipid-peptide mixtures.
Ivanova
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VP
Membrane Thermodynamics Group, Max-Planck-Institute for Biophysical Chemistry, D-37077 Göttingen, Germany.
Heimburg
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D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry; D002151:Calorimetry; D002462:Cell Membrane / Q000378:metabolism*; D006358:Hot Temperature; D008051:Lipid Bilayers / Q000737:chemistry*; D008055:Lipids / Q000737:chemistry; D008566:Membranes / Q000737:chemistry; D008567:Membranes, Artificial; D009010:Monte Carlo Method; D013696:Temperature
D008051:Lipid Bilayers; D008055:Lipids; D008567:Membranes, Artificial; D015060:1,2-Dipalmitoylphosphatidylcholine