title: Gene deletion patterns in spinal muscular atrophy patients with different clinical phenotypes.
abstract: Spinal muscular atrophy (SMA) is an autosomal recessive disorder characterized by degeneration of lower motor neurons. We have assayed deletions in two candidate genes, the survival motor neuron (SMN) and neuronal apoptosis inhibitory protein (NAIP) genes, in 108 samples, of which 46 were from SMA patients, and 62 were from unaffected subjects. The SMA patients included 3 from Bahrain, 9 from South Africa, 2 from India, 5 from Oman, 1 from Saudi Arabia, and 26 from Kuwait. SMN gene exons 7 and 8 were deleted in all type I SMA patients. NAIP gene exons 5 and 6 were deleted in 22 of 23 type I SMA patients. SMN gene exon 7 was deleted in all type II SMA patients while exon 8 was deleted in 19 of 21 type II patients. In 1 type II SMA patient, both centromeric and telomeric copies of SMN exon 8 were deleted. NAIP gene exons 5 and 6 were deleted in only 1 type II SMA patient. In 1 of the 2 type III SMA patients, SMN gene exons 7 and 8 were deleted with no deletion in the NAIP gene, while in the second patient, deletions were detected in both SMN and NAIP genes. None of the 62 unaffected subjects had deletions in either the SMN or NAIP gene. The incidence of biallelic polymorphism in SMN gene exon 7 (BsmAI) was found to be similar (97%) to that (98%) reported in a Spanish population but was significantly different from that reported from Taiwan (0%). The incidence of a second polymorphism in SMN gene exon 8 (presence of the sequence ATGGCCT) was markedly different in our population (97%) and those reported from Spain (50%) and Taiwan (0%).
authors: Paediatrics Department, Faculty of Medicine, Kuwait University, PO Box 24923, Safat 13110, Kuwait. haider@hsc.kuniv.edu.kw
authors: M Z
authors: MZ
authors: Haider
authors: A
authors: A
authors: Moosa
authors: H
authors: H
authors: Dalal
authors: Y
authors: Y
authors: Habib
authors: L
authors: L
authors: Reynold
mesh_terms: D017668:Age of Onset; D017362:Cyclic AMP Response Element-Binding Protein; D005006:Ethnicity / Q000235:genetics; D005091:Exons / Q000235:genetics; D017353:Gene Deletion; D014644:Genetic Variation / Q000235:genetics; D006801:Humans; D007223:Infant; D008969:Molecular Sequence Data; D009134:Muscular Atrophy, Spinal / Q000235:genetics* / Q000503:physiopathology*; D009419:Nerve Tissue Proteins / Q000235:genetics*; D051616:Neuronal Apoptosis-Inhibitory Protein; D010641:Phenotype; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics; D016601:RNA-Binding Proteins; D055532:SMN Complex Proteins
chemical_list: D017362:Cyclic AMP Response Element-Binding Protein; C496800:NAIP protein, human; D009419:Nerve Tissue Proteins; D051616:Neuronal Apoptosis-Inhibitory Protein; D016601:RNA-Binding Proteins; D055532:SMN Complex Proteins