title: Electrophysiological analysis of responses of adult females of Brugia pahangi to some chemicals.
abstract: Electrophysiological techniques were used to obtain recordings of extracellular electrical activity from the anterior end of live adult females of Brugia pahangi. Stimulation with 100 mM, 10 mM and 1 mM acetylcholine resulted in an increase in spike activity which was concentration dependent, whereas stimulation with phosphate-buffered saline and 0.1 mM acetylcholine gave no increase in activity. The delay in response was not concentration dependent. The action of possible host cues was investigated. Stimulation with heat-inactivated foetal calf serum (IFCS) and 10 mM glutathione gave an increase in spike activity but exposure to 5 mg/dl haemoglobin elicited no response. The response to IFCS was found to be suppressed completely by pre-incubation for 30 min in ivermectin.
authors: Entomology and Nematology Department, IACR-Rothamsted, Harpenden, Herts, UK.
authors: R N
authors: RN
authors: Rolfe
authors: J
authors: J
authors: Barrett
authors: R N
authors: RN
authors: Perry
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D000981:Antiprotozoal Agents / Q000494:pharmacology; D017179:Brugia pahangi / Q000187:drug effects* / Q000502:physiology*; D018678:Cholinergic Agents / Q000494:pharmacology; D004594:Electrophysiology; D005260:Female; D005978:Glutathione / Q000494:pharmacology; D006358:Hot Temperature; D007559:Ivermectin / Q000494:pharmacology
chemical_list: D000981:Antiprotozoal Agents; D018678:Cholinergic Agents; D007559:Ivermectin; D005978:Glutathione; D000109:Acetylcholine