title: The leucine zipper motif of the envelope glycoprotein ectodomain of human immunodeficiency virus type 1 contains conformationally flexible regions as revealed by NMR and circular dichroism studies in different media.
abstract: A 43-mer peptide derived from the coiled coil domain of the transmembrane glycoprotein, gp41, of human immunodeficiency virus type 1, was synthesized. Light scattering measurements suggested that the peptide molecules likely exist in the aqueous solution in trimeric form. Circular dichroism experiments showed a moderate helix population enhancement for the peptide in 80% methanol solution relative to helicity in sodium dodecyl sulfate micellar suspension. NMR spectroscopy indicated that the N-terminal section of the peptide was conformationally more sensitive to the medium. The conformationally labile regions contain residues implicated in gp41-gp120 association. Our data support the idea that the coiled coil region is responsible for oligomerization of the gp41 ectodomain and suggest a site of conformational isomerization following receptor binding-induced gp120 dissociation from gp41.
authors: Institute of Chemistry, Academia Sinica, Taipei, Taiwan, Republic of China. dkc@chem.sinica.edu.tw
authors: D K
authors: DK
authors: Chang
authors: V D
authors: VD
authors: Trivedi
authors: S F
authors: SF
authors: Cheng
authors: S
authors: S
authors: Francis
mesh_terms: D000595:Amino Acid Sequence; D002942:Circular Dichroism; D015699:HIV Envelope Protein gp120 / Q000378:metabolism; D015700:HIV Envelope Protein gp41 / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D015497:HIV-1 / Q000737:chemistry*; D016350:Leucine Zippers; D009682:Magnetic Resonance Spectroscopy; D000432:Methanol / Q000737:chemistry; D008969:Molecular Sequence Data; D009154:Mutation; D011487:Protein Conformation; D020449:Repetitive Sequences, Amino Acid; D012542:Scattering, Radiation; D012997:Solvents
chemical_list: D015699:HIV Envelope Protein gp120; D015700:HIV Envelope Protein gp41; D012997:Solvents; D000432:Methanol