title: Conservation of chromosome 1 in turtles over 66 million years.
abstract: Fluorescence in situ hybridization of a whole chromosome 1-specific probe from the yellow-bellied slider turtle (Trachemys scripta) to cells from four other species of turtle ranging from a desert tortoise to a loggerhead sea turtle resulted in specific and exclusive hybridization to chromosome 1 in all five species. Previous observations of conservation in the giemsa banding pattern and chromosome morphology and number among turtles are thus extended to the DNA sequence level, revealing a cytogenetic stability of chromosome 1 in these turtles during the past 66-144 million years. This contrasts with the situation for various hominoid species where, in many instances, extensive chromosomal rearrangements have been reported in one third of that time period. Our probe, which was prepared by microdissecting whole chromosomes from embryonic T. scripta fibroblasts and amplifying using DOP-PCR, is the first report of a whole-chromosome FISH probe for any reptile.
authors: Department of Radiological Health Sciences, Colorado State University, Fort Collins, CO 80523, USA.
authors: M C
authors: MC
authors: Mühlmann-Díaz
authors: B A
authors: BA
authors: Ulsh
authors: F W
authors: FW
authors: Whicker
authors: T G
authors: TG
authors: Hinton
authors: J D
authors: JD
authors: Congdon
authors: J F
authors: JF
authors: Robinson
authors: J S
authors: JS
authors: Bedford
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002871:Chromosome Banding; D020223:Chromosome Painting; D002875:Chromosomes / Q000235:genetics*; D017124:Conserved Sequence / Q000235:genetics*; D015342:DNA Probes / Q000235:genetics; D019143:Evolution, Molecular; D005347:Fibroblasts; D007621:Karyotyping; D010802:Phylogeny; D013997:Time Factors; D014426:Turtles / Q000235:genetics*
chemical_list: D015342:DNA Probes