Cloning and characterization of TNKL, a member of tankyrase gene family.
By serological screening of a breast tumor cDNA library we have identified a novel human gene, tnkl, encoding an ankyrin-related protein with a high degree of similarity to tankyrase, the poly(ADP-ribose)polymerase associated with human telomeres (Smith et al, Science 282: 1484). The tnkl gene maps to chromosome 10, while the tnks gene encoding tankyrase is located on chromosome 8. The predicted 1166-aa protein product of the tnkl gene is 78% identical to human tankyrase and 62% to a putative D. melanogaster protein. Since the proteins have essentially identical domain structures, the corresponding genes form a distinct gene family. The possible link between TNKL and cancer justifies its further functional analysis.
Kuimov
A N
AN
Laboratory of Molecular Immunology, A.N. Belozersky Institute of Physico-Chemical Biology and Center for Molecular Medicine, Moscow State University, Russia.
Kuprash
D V
DV
Petrov
V N
VN
Vdovichenko
K K
KK
Scanlan
M J
MJ
Jongeneel
C V
CV
Lagarkova
M A
MA
Nedospasov
S A
SA
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary; D006801:Humans; D008969:Molecular Sequence Data; D011065:Poly(ADP-ribose) Polymerases / Q000737:chemistry / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D038501:Tankyrases
D017931:DNA Primers; D018076:DNA, Complementary; D011065:Poly(ADP-ribose) Polymerases; D038501:Tankyrases; C418109:TNKS protein, human