Effect of the glycine modulatory site of the N-methyl-D-aspartate receptor on synaptic responses in kitten visual cortex.
In visual cortical slices taken from kittens, the administration of D-serine, an agonist of the N-methyl-D-aspartate receptor complex, significantly enhanced synaptically evoked responses using field potential recordings in lower layer II/III. Expression of normally appearing long-term potentiation (LTP) took place in the presence of the agonist during high-frequency stimulation (HFS). The administration of an antagonist of this receptor, 7-chloro-kynurenic acid (7-Cl KY) alone had no appreciable effect on low-frequency synaptic transmission while HFS failed to induce LTP. Combined administration of D-serine and 7-Cl KY resulted in no alteration of low-frequency synaptic transmission, although expression of LTP was normally obtained. These results suggest that in visual cortex of kittens, the availability of this glycine binding site of the NMDA receptor is necessary for the expression of LTP.
Ito
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Department of Physiology, Yamagata University School of Medicine, 2-2-2 Iida Nishi, 990-9585, Yamagata, Japan. kito@med.id.yamagata-u.ac.jp
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D000818:Animals; D001665:Binding Sites / Q000187:drug effects / Q000502:physiology; D002415:Cats; D004558:Electric Stimulation; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D005260:Female; D005998:Glycine / Q000378:metabolism; D007736:Kynurenic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D017774:Long-Term Potentiation / Q000187:drug effects / Q000502:physiology*; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D018009:Receptors, Glycine / Q000187:drug effects / Q000378:metabolism*; D016194:Receptors, N-Methyl-D-Aspartate / Q000819:agonists / Q000037:antagonists & inhibitors / Q000378:metabolism*; D012694:Serine / Q000494:pharmacology; D013569:Synapses / Q000187:drug effects / Q000378:metabolism; D009435:Synaptic Transmission / Q000187:drug effects / Q000502:physiology*; D014793:Visual Cortex / Q000187:drug effects / Q000378:metabolism*
D018691:Excitatory Amino Acid Antagonists; D018009:Receptors, Glycine; D016194:Receptors, N-Methyl-D-Aspartate; D012694:Serine; D007736:Kynurenic Acid; C057013:7-chlorokynurenic acid; D005998:Glycine