Ineffectiveness of activated carbon in reducing the alteration of sphingolipid metabolism in rats exposed to fumonisin-contaminated diets.
Sphinganine/sphingosine (SA/SO) ratio, a biomarker of fumonisin exposure, has been measured in urine, kidney and liver of male Wistar rats exposed to fumonisin-contaminated diet with and without the addition of activated carbon (AC). The latter was previously shown to adsorb fumonisin B(1) in vitro. Rats were fed either control diet or fumonisin-contaminated diet (4 microg FB(1+)FB(2)/g) or fumonisin-contaminated diet mixed with 20 mg AC/g diet for 1 week. In rats fed fumonisin-contaminated diet, the SA concentration and SA/SO ratio increased significantly and reversibly in kidney, while urine and liver did not show a significant increase of SA/SO ratio. The addition of AC to the fumonisin-contaminated diet did not alter the change of SA/SO biomarker for fumonisin exposure. This provides indications that the use of AC to reduce the toxicity of fumonisins is unlikely to be effective in vivo.
Solfrizzo
M
M
Istituto Tossine e Micotossine da Parassiti Vegetali, CNR, V.le L. Einaudi, 51-70125, Bari, Italy. itmpms12@area.area.ba.cnr.it
Carratu
M R
MR
Avantaggiato
G
G
Galvano
F
F
Pietri
A
A
Visconti
A
A
D000821:Animal Feed; D000818:Animals; D001835:Body Weight; D002606:Charcoal; D004032:Diet; D005506:Food Contamination; D007668:Kidney / Q000378:metabolism; D008297:Male; D009183:Mycotoxins / Q000302:isolation & purification / Q000378:metabolism*; D009929:Organ Size; D051381:Rats; D017208:Rats, Wistar; D013107:Sphingolipids / Q000378:metabolism*; D013110:Sphingosine / Q000031:analogs & derivatives* / Q000378:metabolism
D009183:Mycotoxins; D013107:Sphingolipids; D002606:Charcoal; D013110:Sphingosine; C005682:safingol