title: Dynamic changes in mouse lipoproteins induced by transiently expressed human phospholipid transfer protein (PLTP): importance of PLTP in prebeta-HDL generation.
abstract: The plasma phospholipid transfer protein (PLTP) plays an important role in the regulation of plasma high density lipoprotein (HDL) levels and governs the distribution of HDL sub-populations. In the present study, adenovirus mediated overexpression of human PLTP in mice was employed to investigate the distribution of PLTP in serum and its effect on plasma lipoproteins. Gel filtration experiments showed that the distributions of PLTP activity and mass in serum are different, suggesting that human PLTP circulated in mouse plasma as two distinct forms, one with high and the other with low specific activity. Our study further demonstrates that overexpression of PLTP leads to depletion of HDL and that, as PLTP activity declines, replenishment of the HDL fraction occurs. During this process, the lipoprotein profile displays transient particle populations, including apoA-IV and apoE-rich particles in the LDL size range and small particles containing apoA-II only. The possible role of these particles in HDL reassembly is discussed. The increased PLTP activity enhanced the ability of mouse sera to produce pre(beta)-HDL. The present results provide novel evidence that PLTP is an important regulator of HDL metabolism and plays a central role in the reverse cholesterol transport (RCT) process.
authors: Department of Biochemistry, National Public Health Institute, Mannerheimintie 166, 00300, Helsinki, Finland.
authors: S
authors: S
authors: Jaari
authors: K W
authors: KW
authors: van Dijk
authors: V M
authors: VM
authors: Olkkonen
authors: A
authors: A
authors: van der Zee
authors: J
authors: J
authors: Metso
authors: L
authors: L
authors: Havekes
authors: M
authors: M
authors: Jauhiainen
authors: C
authors: C
authors: Ehnholm
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000257:Adenoviridae Infections / Q000097:blood; D000818:Animals; D016632:Apolipoprotein A-I / Q000097:blood / Q000187:drug effects / Q000378:metabolism; D016633:Apolipoprotein A-II / Q000097:blood / Q000187:drug effects / Q000378:metabolism; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D005822:Genetic Vectors / Q000008:administration & dosage; D006801:Humans; D007267:Injections; D008074:Lipoproteins / Q000097:blood / Q000187:drug effects*; D008075:Lipoproteins, HDL / Q000097:blood / Q000187:drug effects; D008297:Male; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D051379:Mice / Q000097:blood*; D008810:Mice, Inbred C57BL; D049294:Phospholipid Transfer Proteins
chemical_list: D016632:Apolipoprotein A-I; D016633:Apolipoprotein A-II; D002352:Carrier Proteins; D008074:Lipoproteins; D008075:Lipoproteins, HDL; D008565:Membrane Proteins; D049294:Phospholipid Transfer Proteins