Involvement of disregulated c-myc but not c-sis/PDGF in atypical and anaplastic meningiomas.
We investigated the expression of c-myc and c-sis/PDGF mRNA and protein products in 20 cases of meningiomas of various grades: 10 benign, 5 atypical and 5 anaplastic meningiomas. All cases of atypical and anaplastic meningiomas were positive for c-myc protein and mRNA by immunohistochemistry and in situ hybridisation, respectively, while all 10 benign meningiomas were negative for c-myc immunostaining, with only one benign tumour positive for c-myc mRNA. Expression of PDGF-BB protein and c-sis mRNA were seen in more than 80% of the meningioma cases and was not restricted to the histological grades of meningiomas. Semiquantitative analysis showed that the frequency of c-myc immunopositive cells positively correlated with Ki-67 proliferative indices. Our findings suggest that c-myc, but not c-sis/PDGF, has some concern to the malignancy of meningiomas.
Nagashima
G
G
Department of Neurosurgery, Tokyo Medical and Dental University, Tokyo, Japan. goro-n@po1.dti2.ne.jp
Aoyagi
M
M
Yamamoto
S
S
Wakimoto
H
H
Tamaki
M
M
Yamamoto
K
K
Fujimoto
T
T
Hirakawa
K
K
D000328:Adult; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D008577:Meningeal Neoplasms / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000473:pathology*; D008579:Meningioma / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000473:pathology*; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000032:analysis* / Q000235:genetics; D010750:Phosphoproteins / Q000032:analysis* / Q000235:genetics; D016271:Proto-Oncogene Proteins c-myc / Q000032:analysis* / Q000235:genetics; D020574:Proto-Oncogene Proteins c-sis / Q000032:analysis* / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis
D009363:Neoplasm Proteins; C077773:PDGF-related protein, human; D010750:Phosphoproteins; D016271:Proto-Oncogene Proteins c-myc; D020574:Proto-Oncogene Proteins c-sis; D012333:RNA, Messenger