title: Indexing-based differential display--studies on post-mortem Alzheimer's brains.
abstract: In this study we demonstrate for the first time that a novel indexing-based differential display technique generates valid and reproducible results when applied to human post-mortem tissue. We studied expression profiles in prefrontal cortex tissue derived from Alzheimer's disease (AD) and control brains, respectively, and found robust changes in several expressed genes, some of which have a known association with the disease process in AD. These included the dramatic reduction of calcineurin (known to be involved in tau phosphorylation) and GAP-43 (associated with synapse remodelling). Differential display results were confirmed by semi-quantitative RT-PCR on a larger number of brains.
authors: Department of Neurobiology, Babraham Institute, Cambridge CB2 4AT, UK.
authors: M
authors: M
authors: Ryan
authors: M
authors: M
authors: Starkey
authors: R
authors: R
authors: Faull
authors: P
authors: P
authors: Emson
authors: S
authors: S
authors: Bahn
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000209:etiology / Q000235:genetics* / Q000503:physiopathology*; D001923:Brain Chemistry / Q000235:genetics*; D019703:Calcineurin / Q000235:genetics; D005260:Female; D019922:GAP-43 Protein / Q000235:genetics; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D008297:Male
chemical_list: D019922:GAP-43 Protein; D019703:Calcineurin