title: Ultrastructural and permeability features of microvessels in the periventricular area of senescence-accelerated mice (SAM).
abstract: Brain transfer of intravenously injected horseradish peroxidase (HRP) and the ultrastructural features of the vessels were examined in periventricular areas in senescence-accelerated mice (SAMP8), which show age-related deficits in learning and memory, and senescence-accelerated resistant mice (SAMR1), which do not show age-related deficits. In all mice examined with light microscopy, staining reaction for HRP was seen in the periventricular area adjacent to the medial side of the lateral ventricle. Electron microscopic examination in the periventricular area of young and old mice of both strains showed that the staining reaction for HRP appeared in the vesicular profiles of the endothelial cytoplasm, the cytoplasm of the perivascular cells, the basal lamina, and the adjoining extracellular spaces of the white matter, suggesting an incomplete blood-brain barrier (BBB) in the periventricular white matter. In addition, irregularly thickened endothelial cell cytoplasm, membranous inclusions within the basal lamina, and electron-dense endothelial cell cytoplasm were occasionally seen in aged SAMP8 mice. These findings were not observed in 3-month-old SAMP8 mice and 3- and 13-month-old SAMR1 mice. Perivascular collagen deposits were also frequently seen in aged SAMP8 mice. These findings indicate that the endothelial cells and pericytes in the periventricular white matter in aged SAMP8 mice have an ultrastructure with damaged BBB function. Intravascular substances can easily penetrate the periventricular white matter and the BBB of the vessels in the area can be deteriorated with aging in SAMP8 mice.
authors: Department of Pathology II, Kagawa Medical University, Kagawa, Japan. masaueno@kms.ac.jp
authors: M
authors: M
authors: Ueno
authors: H
authors: H
authors: Sakamoto
authors: K
authors: K
authors: Kanenishi
authors: M
authors: M
authors: Onodera
authors: I
authors: I
authors: Akiguchi
authors: M
authors: M
authors: Hosokawa
mesh_terms: D000375:Aging / Q000473:pathology* / Q000502:physiology; D000818:Animals; D001812:Blood-Brain Barrier; D001921:Brain / Q000098:blood supply* / Q000648:ultrastructure; D002199:Capillary Permeability; D002552:Cerebral Ventricles; D006735:Horseradish Peroxidase; D007275:Injections, Intravenous; D007858:Learning; D008568:Memory; D051379:Mice; D008815:Mice, Inbred Strains; D008833:Microcirculation; D008854:Microscopy, Electron
chemical_list: D006735:Horseradish Peroxidase