Identification and functional analysis of an interaction between domains of the 126/183-kDa replicase-associated proteins of tobacco mosaic virus.
The Tobacco mosaic virus (TMV) 126-kDa and read-through 183-kDa replicase-associated proteins have been shown to interact [Watanabe, T., Honda, A., Iwata, A., Ueda, S., Hibi, T., Ishihama, A. (1999). J. Virol. 73, 2633-2640]. To identify and investigate the sequence required for this interaction, five segments covering different portions of the 126/183-kDa open reading frame, including the methyl-transferase, intervening region (IR), helicase-like (HEL), and polymerase domains, were screened via the yeast two-hybrid system against a library of TMV protein segments. Only one specific interaction between the HEL domain clone and a TMV library clone, IRnHEL, encoding the C-terminal half of the IR and the N-terminal portion of the HEL domain was identified. Sequence and deletion analysis revealed that the interacting clones share a region containing the helicase NTP-binding motif and that this region was essential for the interaction. To determine the functional significance of this interaction, mutants of the HEL domain segment that conferred a temperature-sensitive (ts) defect in the yeast interaction were identified and cloned into a recombinant TMV strain. Of the five selected mutants, three (V823I/S824N/V1042M, A877V, V1087I) produced a ts replication phenotype in protoplasts while the other two (A1073V, T884I) abolished TMV replication at both the permissive and the nonpermissive temperatures. An additional mutation, K839S, designed to disrupt the shared NTP-binding motif, nearly abolished the two-hybrid interaction and prevented virus replication, suggesting that NTP-binding and/or the structure of this motif is a contributing factor in the interaction. Taken together, these results provide support for an interaction between TMV replicase-associated proteins that involves specific structural features of the HEL and IR domains.
Goregaoker
S P
SP
Molecular and Cell Biology Program, University of Maryland, College Park, Maryland 20742, USA.
Lewandowski
D J
DJ
Culver
J N
JN
D020816:Amino Acid Motifs; D019943:Amino Acid Substitution / Q000235:genetics; D001665:Binding Sites; D004265:DNA Helicases / Q000737:chemistry; D008780:Methyltransferases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008970:Molecular Weight; D009097:Multienzyme Complexes / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D010641:Phenotype; D010935:Plant Diseases / Q000821:virology; D018515:Plant Leaves / Q000821:virology; D010947:Plants, Toxic; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D012367:RNA, Viral / Q000096:biosynthesis / Q000235:genetics; D012324:RNA-Dependent RNA Polymerase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D017384:Sequence Deletion / Q000235:genetics; D013696:Temperature; D014026:Nicotiana / Q000821:virology*; D014027:Tobacco Mosaic Virus / Q000737:chemistry / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D020798:Two-Hybrid System Techniques; D014779:Virus Replication
D009097:Multienzyme Complexes; D012367:RNA, Viral; D008780:Methyltransferases; D012324:RNA-Dependent RNA Polymerase; D004265:DNA Helicases