title: "Red complex" (Bacteroides forsythus, Porphyromonas gingivalis, and Treponema denticola) in endodontic infections: a molecular approach.
abstract: OBJECTIVE
  The "red complex," composed of Bacteroides forsythus, Porphyromonas gingivalis, and Treponema denticola, is implicated in severe forms of periodontal diseases. The purpose of this study was to assess the occurrence of the red complex in root canal infections through the use of a sensitive technique-the 16S rDNA-directed polymerase chain reaction (PCR).
  
  
  METHODS
  Samples were obtained from 50 necrotic pulps with periradicular pathosis. Ten cases were diagnosed as acute periradicular abscesses. DNA was extracted from the samples and analyzed with a PCR-based identification assay.
  
  
  RESULTS
  At least 1 member of the red complex was found in 33 of 50 cases. T denticola, P gingivalis, and B forsythus were detected in 44%, 30%, and 26% of the cases, respectively. The red complex was found in 4 of 50 cases. No particular signs or symptoms were associated with the presence of these bacterial species.
  
  
  CONCLUSIONS
  Despite what is indicated in reports with respect to marginal periodontitis, red complex bacteria-either singularly or collectively-was not associated with any particular pattern of clinical symptoms. However, because the bacterial species from the red complex are recognized oral pathogens, their occurrence in root canal infections suggests that they may play a role in the pathogenesis of periradicular diseases.
authors: Department of Endodontics, School of Dentistry, Estácio de Sá University, Rio de Janeiro, Brazil.
authors: I N
authors: IN
authors: Rôças
authors: J F
authors: JF
authors: Siqueira
authors: K R
authors: KR
authors: Santos
authors: A M
authors: AM
authors: Coelho
mesh_terms: D000293:Adolescent; D000328:Adult; D001424:Bacterial Infections / Q000235:genetics; D001439:Bacteroides / Q000302:isolation & purification / Q000472:pathogenicity*; D016009:Chi-Square Distribution; D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D006801:Humans; D008875:Middle Aged; D010482:Periapical Abscess / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D016966:Porphyromonas gingivalis / Q000302:isolation & purification / Q000472:pathogenicity*; D014208:Treponema / Q000302:isolation & purification / Q000472:pathogenicity*
chemical_list: D015342:DNA Probes; D004269:DNA, Bacterial; D004275:DNA, Ribosomal