Expression of cell cycle-associated proteins CDK4, p27, and E2F-1 in chondrosarcoma of the jaws.
OBJECTIVE
To elucidate the expression and significance of cell cycle-associated proteins in chondrosarcoma of the jaws.


METHODS
CDK4, p27, and E2F-1 expression were examined in chondrosarcoma and osteochondroma of the jaws by immunohistochemical ABC method.


RESULTS
CDK4, p27, and E2F-1 were positive in 60% (12 of 20), 25% (5 of 20), and 65% (13 of 20), respectively, in chondrosarcoma of the jaws. There was no significant difference in the expression of CDK4, P27, and E2F-1 among histological grade of the chondrosarcoma (P > 0.05). In osteochondroma of the jaws, CDK4 and E2F-1 showed an equal positivity of 12.5% (1 of 8), whereas p27 was positive in 87.5% (7 of 8). The positive rate of CDK4, p27, and E2F-1 was significant between chondrosarcoma and osteochondroma of the jaws (P < 0.05).


CONCLUSIONS
These data show that cell cycle regulatory proteins are altered in chondrosarcoma of the jaws. CDK4 and E2F-1 are over-expressed and p27 is under-expressed in chondrosarcoma of the jaws.
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X H
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Department of Endodontics, School of Stomatology, Shanghai Second Medical University, Shanghai 200011, P. R. China.
D048868:Adaptor Proteins, Signal Transducing; D000293:Adolescent; D000328:Adult; D002352:Carrier Proteins / Q000032:analysis* / Q000235:genetics; D018797:Cell Cycle Proteins / Q000032:analysis* / Q000235:genetics; D002648:Child; D002813:Chondrosarcoma / Q000235:genetics / Q000473:pathology*; D004396:Coloring Agents; D051358:Cyclin-Dependent Kinase 4; D050760:Cyclin-Dependent Kinase Inhibitor p27; D018844:Cyclin-Dependent Kinases / Q000032:analysis* / Q000037:antagonists & inhibitors* / Q000235:genetics; D004791:Enzyme Inhibitors / Q000032:analysis*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D016147:Genes, Tumor Suppressor / Q000235:genetics; D006801:Humans; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D008297:Male; D008339:Mandibular Neoplasms / Q000235:genetics / Q000473:pathology*; D008441:Maxillary Neoplasms / Q000235:genetics / Q000473:pathology*; D008869:Microtubule-Associated Proteins / Q000032:analysis* / Q000235:genetics; D008875:Middle Aged; D015831:Osteochondroma / Q000235:genetics / Q000473:pathology; D011518:Proto-Oncogene Proteins; D016601:RNA-Binding Proteins; D025521:Tumor Suppressor Proteins
D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D004396:Coloring Agents; D004791:Enzyme Inhibitors; D008869:Microtubule-Associated Proteins; C101633:PA2G4 protein, human; D011518:Proto-Oncogene Proteins; D016601:RNA-Binding Proteins; D025521:Tumor Suppressor Proteins; D050760:Cyclin-Dependent Kinase Inhibitor p27; C495900:CDK4 protein, human; D051358:Cyclin-Dependent Kinase 4; D018844:Cyclin-Dependent Kinases