Effect of dietary lipid source on conjugated linoleic acid concentrations in milk fat.
Conjugated linoleic acids (CLA) found in ruminant milk fat are a byproduct of incomplete biohydrogenation of lipids by ruminal bacteria. We examined the effect of different dietary fat supplements and processing methods on CLA. In trial 1, dietary supplements of Ca salts of fatty acids from canola oil, soybean oil, and linseed oil increased CLA content of milk fat by three- to fivefold over the control diet. Trials 2 and 3 examined the effect of processing methods for heat treatment of full fat soybeans. In trial 2, extrusion, micronizing, and roasting resulted in two- to threefold greater concentrations of CLA in milk fat than the control diet (raw ground soybeans). In trial 3, different temperatures of extrusion (120, 130, and 140 degrees C) increased the CLA content of milk fat to a similar extent; CLA averaged 19.9 mg/g of fatty acids for the extrusion treatments compared with 4.2 mg/g of fatty acids for the control diet (raw ground soybeans). Fish oil (200 and 400 ml/d) was examined in trial 4 and both levels resulted in CLA concentrations in milk fat that were about threefold greater than the control diet. In trial 5, grain and silage from a high oil corn hybrid increased the CLA content of milk fat; however, responses were modest with the CLA concentration (mg/g of fatty acids) averaging 4.6 and 2.8 for diets with high oil hybrid and normal hybrid, respectively. Similarly, dietary supplements of animal fat byproducts (tallow plus yellow grease; trial 6) resulted in modest increases in the CLA content of milk fat. Overall, several dietary manipulations involving lipid sources and processing methods were identified that allow for a marked increase in the conjugated linoleic acid content of milk fat.
Chouinard
P Y
PY
Department of Animal Science, Cornell University, Ithaca, NY 14853, USA.
Corneau
L
L
Butler
W R
WR
Chilliard
Y
Y
Drackley
J K
JK
Bauman
D E
DE
D000821:Animal Feed; D000818:Animals; D002417:Cattle; D004041:Dietary Fats / Q000008:administration & dosage*; D005229:Fatty Acids, Monounsaturated / Q000008:administration & dosage; D005260:Female; D005395:Fish Oils / Q000008:administration & dosage; D005511:Food Handling / Q000379:methods; D006358:Hot Temperature; D019787:Linoleic Acid / Q000032:analysis* / Q000096:biosynthesis; D008043:Linseed Oil / Q000008:administration & dosage; D008892:Milk / Q000737:chemistry*; D000074262:Rapeseed Oil; D013024:Soybean Oil / Q000008:administration & dosage
D004041:Dietary Fats; D005229:Fatty Acids, Monounsaturated; D005395:Fish Oils; D000074262:Rapeseed Oil; D013024:Soybean Oil; D008043:Linseed Oil; D019787:Linoleic Acid