Intracameral and lenticular penetration of locally applied stable isotope-labeled vitamin E.
OBJECTIVE
To confirm the aqueous humor and lens dynamics of 1% deuterium-labeled alpha-tocopherol acetate (D(3)-VEA) solution.


METHODS
The concentrations of D(3)-VEA and D(3)-alpha-tocopherol (D(3)-VE), derived from D(3)-VEA, in the aqueous humor and lens were measured after continuous instillation of 1% D(3)-VEA into the cul-de-sac of rat eyes for 1 or 3 weeks. D(3)-VEA and D(3)-VE concentrations were determined by gas chromatography/mass selective detector.


RESULTS
In the aqueous humor, the concentrations of D(3)-VEA and D(3)-VE were, respectively, 93.1 and 9.4 ng/mL after 1 week, and, respectively, 498.9 and 21.5 ng/mL after 3 weeks. In the lens, they were, respectively, 15.0 and 9.8 ng/g after 1 week, and 6.1 and 4.8 ng/g after 3 weeks.


CONCLUSIONS
The penetration levels of alpha-tocopherol acetate by eyedrop application were confirmed in the aqueous humor and lens.
Nagata
M
M
Department of Ophthalmology, Kanazawa Medical University, Uchinada, Ishikawa, Japan.
Kawazu
K
K
Midori
Y
Y
Kojima
M
M
Shirasawa
E
E
Sasaki
K
K
D000287:Administration, Topical; D000818:Animals; D001082:Aqueous Humor / Q000378:metabolism*; D003903:Deuterium; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D007553:Isotope Labeling; D007908:Lens, Crystalline / Q000378:metabolism*; D009883:Ophthalmic Solutions; D051381:Rats; D011914:Rats, Inbred BN; D014018:Tissue Distribution; D014810:Vitamin E / Q000493:pharmacokinetics*
D009883:Ophthalmic Solutions; D014810:Vitamin E; D003903:Deuterium