title: Corneal and stomach expression of aldehyde dehydrogenases: from fish to mammals.
abstract: We have studied the distribution of the ALDH3A1, ALDH1A1 and ALDH2 proteins in the cornea and stomach of several animal species, including mammals (C57BL/6J and SWR/J mice, rat and pig), birds (chicken and turkey), amphibians (frog) and fish (trout and zebrafish). High ALDH3A1 protein levels and catalytic activities were detected in C57BL/6J mouse, rat and pig. We found complete absence of the ALDH3A1 protein in SWR/J mice, which carry the Aldh3a1(c) allele characterized by four amino acid substitutions (G88R, I154N, H305R and I352V) and lack of enzymatic activity. This indicates that the SWR/J mouse strain is a natural gene knockout model for ALDH3A1. Traces of ALDH3A1 were detected in rabbit, whereas expression was absent from chicken, turkey, frog, trout, and zebrafish. Interestingly, significant levels of the cytosolic ALDH1A1 and mitochondrial ALDH2 proteins were detected by immunoblot analysis in all examined species that are deficient in ALDH3A1 expression. In contrast, no ALDH1A1 or ALDH2 protein was detected in the species expressing ALDH3A1. It can, therefore, be concluded that corneal expression of ALDH3A1 or ALDH1A1/ALDH2 occurs in a taxon-specific manner, supporting the protective role of these ALDHs in cornea against the UV-induced oxidative damage.
authors: Molecular Toxicology and Environmental Health Sciences, Department of Pharmaceutical Sciences, University of Colorado Health Sciences Center, 80262, Denver, CO, USA.
authors: A
authors: A
authors: Pappa
authors: N A
authors: NA
authors: Sophos
authors: V
authors: V
authors: Vasiliou
mesh_terms: D000444:Aldehyde Dehydrogenase / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001001:Anura; D002645:Chickens; D003315:Cornea / Q000201:enzymology*; D019143:Evolution, Molecular; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D005810:Multigene Family; D010802:Phylogeny; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley; D013045:Species Specificity; D013270:Stomach / Q000201:enzymology*; D013552:Swine; D014337:Trout; D014422:Turkeys; D015027:Zebrafish
chemical_list: D007527:Isoenzymes; D000444:Aldehyde Dehydrogenase