Identification and localization of a thylakoid-bound carbonic anhydrase from the green algae Tetraedron minimum (Chlorophyta) and Chlamydomonas noctigama (Chlorophyta).
In order to broaden our understanding of the eukaryotic CO2-concentrating mechanism the occurrence and localization of a thylakoid-associated carbonic anhydrase (EC 4.2.1.1) were studied in the green algae Tetraedron minimum and Chlamydomonas noctigama. Both algae induce a CO2-concentrating mechanism when grown under limiting CO2 conditions. Using mass-spectrometric measurements of 18O exchange from doubly labelled CO2, the presence of a thylakoid-associated carbonic anhydrase was confirmed for both species. From purified thylakoid membranes, photosystem I (PSI), photosystem II (PSII) and the light-harvesting complex of the photosynthetic apparatus were isolated by mild detergent gel. The protein fractions were identified by 77 K fluorescence spectroscopy and immunological studies. A polypeptide was found to immunoreact with an antibody raised against thylakoid carbonic anhydrase (CAH3) from Chlamydomonas reinhardtii. It was found that this polypeptide was mainly associated with PSII, although a certain proportion was also connected to light harvesting complex II. This was confirmed by activity measurements of carbonic anhydrase in isolated bands extracted from the mild detergent gel. The thylakoid carbonic anhydrase isolated from T. minimum had an isoelectric point between 5.4 and 4.8. Together the results are consistent with the hypothesis that thylakoid carbonic anhydrase resides within the lumen where it is associated with the PSII complex.
van Hunnik
E
E
Institute for Molecular Cell Biology, BioCentrum Amsterdam, University of Amsterdam, The Netherlands. hunnik@bio.uva.nl
Livne
A
A
Pogenberg
V
V
Spijkerman
E
E
van den Ende
H
H
Mendoza
E G
EG
Sültemeyer
D
D
de Leeuw
J W
JW
D000818:Animals; D002245:Carbon Dioxide / Q000737:chemistry / Q000502:physiology; D002256:Carbonic Anhydrases / Q000378:metabolism*; D002696:Chlamydomonas / Q000201:enzymology*; D000460:Chlorophyta / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D015151:Immunoblotting; D066298:In Vitro Techniques; D013058:Mass Spectrometry; D045322:Photosynthetic Reaction Center Complex Proteins / Q000032:analysis / Q000378:metabolism; D045331:Photosystem I Protein Complex; D045332:Photosystem II Protein Complex; D013050:Spectrometry, Fluorescence; D020524:Thylakoids / Q000737:chemistry / Q000201:enzymology*
D045322:Photosynthetic Reaction Center Complex Proteins; D045331:Photosystem I Protein Complex; D045332:Photosystem II Protein Complex; D002245:Carbon Dioxide; D002256:Carbonic Anhydrases