Identification, characterization and leucocyte expression of Siglec-10, a novel human sialic acid-binding receptor.
Here we characterize Siglec-10 as a new member of the Siglec family of sialic acid-binding Ig-like lectins. A full-length cDNA was isolated from a human spleen library and the corresponding gene identified. Siglec-10 is predicted to contain five extracellular Ig-like domains and a cytoplasmic tail containing three putative tyrosine-based signalling motifs. Siglec-10 exhibited a high degree of sequence similarity to CD33-related Siglecs and mapped to the same region, on chromosome 19q13.3. The expressed protein was able to mediate sialic acid-dependent binding to human erythrocytes and soluble sialoglycoconjugates. Using specific antibodies, Siglec-10 was detected on subsets of human leucocytes including eosinophils, monocytes and a minor population of natural killer-like cells. The molecular properties and expression pattern suggest that Siglec-10 may function as an inhibitory receptor within the innate immune system.
Munday
J
J
The Wellcome Trust Biocentre, School of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland, U.K.
Kerr
S
S
Ni
J
J
Cornish
A L
AL
Zhang
J Q
JQ
Nicoll
G
G
Floyd
H
H
Mattei
M G
MG
Moore
P
P
Liu
D
D
Crocker
P R
PR
D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D002888:Chromosomes, Human, Pair 19; D017931:DNA Primers; D018076:DNA, Complementary; D006801:Humans; D037102:Lectins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D007962:Leukocytes / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D011956:Receptors, Cell Surface; D017386:Sequence Homology, Amino Acid
D017931:DNA Primers; D018076:DNA, Complementary; D037102:Lectins; D011956:Receptors, Cell Surface; C426228:SIGLEC10 protein, human