Genetic modification of cotton seed oil using inverted-repeat gene-silencing techniques.
Inverted-repeat-based gene constructs targeted against two key cotton seed-specific fatty acid desaturase genes, ghSAD-1, encoding stearoylacvl carrier protein delta9-desaturase and ghFAD2-1, encoding microsomal omega-6 desaturase, were transformed into cotton. The expression of ghSAD-1 and ghFAD2-1 in the inverted-repeat orientation resulted in increased levels of stearic and oleic acids, respectively. Interestingly, the content of palmitic acid in both high-stearic and high-oleic lines was substantially reduced. These materials offer the promise of developing cotton seed oil products with greatly improved nutritional appeal to consumers.
Liu
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CSIRO Plant Industry, Canberra, Australia.
Singh
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Green
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D003369:Cottonseed Oil / Q000737:chemistry; D018744:DNA, Plant / Q000235:genetics*; D044943:Fatty Acid Desaturases / Q000235:genetics; D020868:Gene Silencing; D005818:Genetic Engineering / Q000379:methods*; D003368:Gossypium / Q000201:enzymology / Q000235:genetics*; D019787:Linoleic Acid / Q000032:analysis; D008861:Microsomes / Q000201:enzymology; D006899:Mixed Function Oxygenases / Q000235:genetics; D019301:Oleic Acid / Q000032:analysis; D012091:Repetitive Sequences, Nucleic Acid; D013229:Stearic Acids / Q000032:analysis
D003369:Cottonseed Oil; D018744:DNA, Plant; D013229:Stearic Acids; D019301:Oleic Acid; C031183:stearic acid; D019787:Linoleic Acid; D006899:Mixed Function Oxygenases; D044943:Fatty Acid Desaturases; C018747:acyl-(acyl-carrier-protein)desaturase; C040448:delta-12 fatty acid desaturase