title: Molecular characterization of tomato 3-dehydroquinate dehydratase-shikimate:NADP oxidoreductase.
abstract: Analysis of cDNAs encoding the bifunctional 3-dehydroquinate dehydratase-shikimate:NADP oxidoreductase (DHQase-SORase) from tomato (Lycopersicon esculentum) revealed two classes of cDNAs that differed by 57 bp within the coding regions, but were otherwise identical. Comparison of these cDNA sequences with the sequence of the corresponding single gene unequivocally proved that the primary transcript is differentially spliced, potentially giving rise to two polypeptides that differ by 19 amino acids. Quantitative real-time polymerase chain reaction revealed that the longer transcript constitutes at most 1% to 2% of DHQase-SORase transcripts. Expression of the respective polypeptides in Escherichia coli mutants lacking the DHQase or the SORase activity gave functional complementation only in case of the shorter polypeptide, indicating that skipping of a potential exon is a prerequisite for the production of an enzymatically active protein. The deduced amino acid sequence revealed that the DHQase-SORase is most likely synthesized as a precursor with a very short (13-amino acid) plastid-specific transit peptide. Like other genes encoding enzymes of the prechorismate pathway in tomato, this gene is elicitor-inducible. Tissue-specific expression resembles the patterns obtained for 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase 2 and dehydroquinate synthase genes. This work completes our studies of the prechorismate pathway in that cDNAs for all seven enzymes (including isozymes) of the prechorismate pathway from tomato have now been characterized.
authors: Institute of Plant Sciences, Swiss Federal Institute of Technology, Universitätstrasse 2, CH-8092 Zurich, Switzerland.
authors: M
authors: M
authors: Bischoff
authors: A
authors: A
authors: Schaller
authors: F
authors: F
authors: Bieri
authors: F
authors: F
authors: Kessler
authors: N
authors: N
authors: Amrhein
authors: J
authors: J
authors: Schmid
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D020121:5' Untranslated Regions / Q000235:genetics; D000429:Alcohol Oxidoreductases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000201:enzymology / Q000254:growth & development; D006836:Hydro-Lyases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D007438:Introns; D018551:Solanum lycopersicum / Q000201:enzymology* / Q000235:genetics*; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D015183:Restriction Mapping; D012717:Sesquiterpenes / Q000378:metabolism*; D045788:Sesquiterpenes, Germacrane
chemical_list: D020413:3' Untranslated Regions; C431675:3-dehydroquinate dehydratase-shikimate - NADP oxidoreductase; D020121:5' Untranslated Regions; D009097:Multienzyme Complexes; D011994:Recombinant Proteins; D012717:Sesquiterpenes; D045788:Sesquiterpenes, Germacrane; C431525:germacra-1(10),4,11(13)-trien-12-oic acid; D000429:Alcohol Oxidoreductases; D006836:Hydro-Lyases