[Transplantation of cultured human keratinocyte on collagen sponge].
OBJECTIVE
To investigate the skin regeneration using cultured human keratinocytes with collagen sponge transplanted into thickness wound of nude mice.


METHODS
Human foreskin from foreskin ectomy procedures was detached with 0.5% Dispase II. Epidermis sheets were separated from dermis and digested with 0.05% Trypsin into single cell suspension. Keratinocytes were cultured and seeded into collagen sponge during logarithmic growth phase. After 3 days, the keratinocytes-collagen sponge were grafted on full thickness wound of nude mice, compared with simple collagen sponge without keratinocytes. The histological, immunohistochemical examination and electron microscopy were detected.


RESULTS
After the epidermal substitute was grafted onto wound, the human keratinocytes were able to further proliferate and differentiate and develop into new epithelia. Compared with the control group, the wound healed earlier and contracted less, epithelia matured earlier, and the collagen fiber was less beneath epithelia.


CONCLUSIONS
Keratinocytes can grow on collagen sponge and migrate onto wound to develop into stratified epithelia and inhibit wound contract. The keratinocyte graft can be used to repair skin defect.
Xu
L H
LH
Department of Plastic Surgery, Changhai Hospital, Second Military Medical University, Shanghai, P. R. China 200433.
Jiao
X Y
XY
Ji
Z L
ZL
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D003094:Collagen; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000637:transplantation*; D051381:Rats; D011923:Rats, Nude; D023822:Tissue Engineering / Q000379:methods*
D003094:Collagen