[The influence of tissue engineered tendon on subgroup of T lymphocytes and its receptor in roman chickens].
OBJECTIVE
To investigate the influence of tissue engineered tendon on subgroup of T lymphocytes and its receptor in Roman chickens.


METHODS
The flexor digitorum profundus of the third toes of right feet in 75 Roman chickens were resected and made 2.5 cm defects as experimental model. They were randomly divided into five groups according to five repair methods: no operation (group A), autograft (group B), fresh allograft (group C), polymer combined with allogenous tendon cells (group D), derived tendon materials combined with allogenous tendon cells (group E). The proliferation and transformation of lymphocytes and contribution of CD4+, CD8+, CD28 and T cell receptor (TCR) were detected to study the immune response.


RESULTS
The CD4+, CD8+ and TCR of group D and E were increased slightly than that of group B after 7 days, while after 14 days, those data decreased gradually and no significant difference between tissue engineered tendon and autografts (P > 0.05), and there was significant difference between fresh allograft and tissue engineered tendon (P < 0.05). Lymphocytes transformation induced by conA also showed no significant difference between tissue engineered tendon and autografts (P > 0.05).


CONCLUSIONS
Tendon cells are hypoantigen cells, there are less secretion of soluble antigen or antigen chips dropped out from cells. Tissue engineered tendon has excellent biocompatibility.
Qu
Y L
YL
Department of Orthopedic Surgery, First University Hospital, West China University of Medical Sciences, Chengdu Sichuan, P, R, China 610041. orthop@public.cd.sc.cn
Yang
Z M
ZM
Xie
H Q
HQ
D000818:Animals; D002645:Chickens; D008422:Materials Testing; D011897:Random Allocation; D011948:Receptors, Antigen, T-Cell / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D013710:Tendons / Q000166:cytology / Q000637:transplantation; D023822:Tissue Engineering / Q000379:methods*
D011948:Receptors, Antigen, T-Cell