The metabolism of norethandrolone in the horse: characterization of 16-, 20- and 21-oxygenated metabolites by gas chromatography/mass spectrometry.
After oral administration to a thoroughbred gelding, the anabolic steroid norethandrolone was converted into a complex mixture of oxygenated metabolites. These metabolites were extracted from the urine, deconjugated by methanolysis and converted to their O-methyloxime trimethylsilyl derivatives. Gas chromatographic/mass spectrometric analysis indicated the major metabolites to be 19-norpregnane-3,16,17-triols, 19-norpregnane-3,17,20-triols and 3,17-dihydroxy-19-norpregnan-21-oic acids. Some minor metabolites were also detected.
McKinney
A R
AR
Australian Racing Forensic Laboratory, P.O. Box 528, Kensington, New South Wales 1465, Australia.
Ridley
D D
DD
Suann
C J
CJ
D000818:Animals; D002849:Chromatography, Gas; D006736:Horses / Q000378:metabolism*; D013058:Mass Spectrometry; D009639:Norethandrolone / Q000378:metabolism*; D010084:Oxidation-Reduction
D009639:Norethandrolone