Conformation and orientation of the gene 9 minor coat protein of bacteriophage M13 in phospholipid bilayers.
The membrane-bound state of the gene 9 minor coat protein of bacteriophage M13 was studied in model membrane systems, which varied in lipid head group and lipid acyl chain composition. By using FTIR spectroscopy and subsequent band analysis a quantitative analysis of the secondary structure of the protein was obtained. The secondary structure of the gene 9 protein predominantly consists of alpha-helical (67%) and turn (33%) structures. The turn structure is likely to be located C-terminally where it has a function in recognizing the phage DNA during bacteriophage assembly. Attenuated total reflection FTIR spectroscopy was used to determine the orientation of gene 9 protein in the membrane, revealing that the alpha-helical domain is mainly transmembrane. The conformational and orientational measurements result in two models for the gene 9 protein in the membrane: a single transmembrane helix model and a two-helix model consisting of a 15 amino acid long transmembrane helix and a 10 amino acid long helix oriented parallel to the membrane plane. Potential structural consequences for both models are discussed.
Houbiers
M C
MC
Department of Biomolecular Sciences, Laboratory of Molecular Physics, Wageningen University and Research Center, Dreijenlaan 3 6703 HA Wageningen, The Netherlands. chantal.houbiers@virus.mf.wau.nl
Wolfs
C J
CJ
Spruijt
R B
RB
Bollen
Y J
YJ
Hemminga
M A
MA
Goormaghtigh
E
E
D000595:Amino Acid Sequence; D017104:Bacteriophage M13 / Q000737:chemistry / Q000235:genetics*; D002213:Capsid / Q000737:chemistry / Q000235:genetics*; D036022:Capsid Proteins; D004134:Dimyristoylphosphatidylcholine; D008051:Lipid Bilayers / Q000737:chemistry*; D008969:Molecular Sequence Data; D010715:Phosphatidylglycerols; D010743:Phospholipids / Q000737:chemistry; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D017550:Spectroscopy, Fourier Transform Infrared
D036022:Capsid Proteins; D008051:Lipid Bilayers; D010715:Phosphatidylglycerols; D010743:Phospholipids; C117230:gene 9 minor coat protein, bacteriophage M13; C002773:dimyristoylphosphatidylglycerol; D004134:Dimyristoylphosphatidylcholine