Cobas Mira S endpoint enzymatic assay for plasma formate.
In methanol intoxication, increased levels of the metabolite formate are associated with metabolic acidosis and an increased risk for ocular and neurological dysfunction. A simple method for plasma formate measurement by adaptation of a manual enzymatic assay to a Cobas Mira S analyzer is presented. Six microliters of sample is incubated for 5 min with buffer containing nicotinamide-adenine dinucleotide. Fifteen microliters of a suspension of formate dehydrogenase is then added. Absorbance at 340 nm is measured every 25 s. The NADH produced when formate is oxidized is stoichiometric to the amount of formate. The method is sensitive, reproducible, and specific and has a broad measurement range. The frozen reagents are stable for at least six months, so the described method can be applied to irregular and semi-urgent requests. A recent case is reported.
Blomme
B
B
Department of Clinical Chemistry--Toxicology, Academic Hospital Free University Brussels, Belgium.
Lheureux
P
P
Gerlo
E
E
Maes
V
V
D000138:Acidosis / Q000175:diagnosis / Q000378:metabolism; D000328:Adult; D005560:Formate Dehydrogenases / Q000378:metabolism*; D005561:Formates / Q000097:blood*; D006801:Humans; D007202:Indicators and Reagents; D008297:Male; D000432:Methanol / Q000378:metabolism* / Q000633:toxicity; D009243:NAD / Q000378:metabolism; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D005561:Formates; D007202:Indicators and Reagents; D009243:NAD; D005560:Formate Dehydrogenases; D000432:Methanol