Direct polymerase chain reaction detection of ropy Pediococcus damnosus strains in wine.
OBJECTIVE
Glucan-producing strains of Pediococcus damnosus are considered as spoilage micro-organisms because synthesis of glucan leads to an unacceptable viscosity of wine. In this report, we present a polymerase chain reaction (PCR) procedure to detect the presence of such strains in wines.


RESULTS
We developed a direct DNA isolation method from the wine microflora using polyvinylpyrrolidone in order to decrease the polyphenolic concentration. The sequence of the plasmid involved in glucan production allowed the design of a primer pair usable for a specific and sensitive PCR procedure, leading to the amplification of a 563-bp fragment.


CONCLUSIONS
The detection limit in wine was 102 cfu ml-1. The detection sensitivity could be increased by using a second primer pair in nested PCR assays.


CONCLUSIONS
The method proved to be efficient for the early and sensitive detection of ropy Ped. damnosus strains during wine-making. Time-consuming culture and colony isolation steps are no longer needed.
Gindreau
E
E
Faculté d'Oenologie, Unité Associée INRA-Université Victor Segalen Bordeaux 2, 351 cours de la Libération, 33405 Talence cedex, France. emmanuel.gindreau@oenologie.u-bordeaux2.fr
Walling
E
E
Lonvaud-Funel
A
A
D000595:Amino Acid Sequence; D001483:Base Sequence; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis*; D008969:Molecular Sequence Data; D010376:Pediococcus / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods; D011786:Quality Control; D014920:Wine / Q000382:microbiology*
D017931:DNA Primers; D004269:DNA, Bacterial