Preparative-scale isolation of four anthocyanin components of black currant (Ribes nigrum L.) fruits.
Four anthocyanin components of black currant, delphinidin 3-O-beta-rutinoside (D3R), cyanidin 3-O-beta-rutinoside (C3R), delphinidin 3-O-beta-glucoside (D3G), and cyanidin 3-O-beta-glucoside (C3G), were successfully isolated as crystalline forms on a preparative scale. In this process, selective hydrolysis of the glucosides (D3G and C3G) and rhamnosides (D3R and C3R) was achieved by treatment with beta-glucosidase and hesperidinase (alpha-rhamnosidase), respectively, to improve resolution of anthocyanin components. Especially, selective conversion of the rutinosides into glucosides made the amounts of D3G and C3G increase about 4- and 7-fold, respectively. D3R, C3R, D3G, and C3G were isolated from enzymatic hydrolysates of black currant anthocyanins through Amberlite XAD-7HP absorption followed by preparative HPLC separation, and their crystals were obtained as the flavylium chloride.
Matsumoto
H
H
Bioscience Laboratories, Meiji Seika Kaisha, Ltd., 5-3-1 Chiyoda, Sakado-shi, Saitama 350-0289, Japan. hitoshi_matsumoto@meiji.co.jp
Hanamura
S
S
Kawakami
T
T
Sato
Y
Y
Hirayama
M
M
D000872:Anthocyanins / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D005638:Fruit / Q000737:chemistry*; D006027:Glycosides / Q000032:analysis / Q000737:chemistry; D015394:Molecular Structure
D000872:Anthocyanins; D006027:Glycosides