Scale-up studies on a defined medium process for pilot plant production of illicicolin by Gliocladium roseum.
Illicicolin was cultivated at the 600-L pilot scale for purposes of material generation and process development. The initial medium containing oat flour was difficult operationally as a result of excessive foaming during sterilization, so a new defined medium process (with either glucose or sucrose as the carbon source), developed at the 23-L scale, was scaled up and improved for pilot scale needs. Pilot scale media development efforts focused on exploring the highest concentration of media (1.0 x to 3.0 x) that could be cultivated at the pilot scale and not be limited by mixing or oxygen mass transfer. The process was scaled up successfully and peak titers improved 7.5-fold, from about 200 mg/L in the initial complex medium to 1500 mg/L in the final defined medium.
Junker
B
B
Merck & Co. Inc., RY810-126, P. O. Box 2000, Rahway, NJ 07065, USA.
Zhang
J
J
Mann
Z
Z
Reddy
J
J
Greasham
R
R
D005285:Fermentation; D020462:Gliocladium / Q000737:chemistry*; D010636:Phenols / Q000138:chemical synthesis*; D013395:Sucrose / Q000378:metabolism
D010636:Phenols; C433217:illicicolin; D013395:Sucrose