Affinity chromatography of porcine pepsin on different types of immobilized 3,5-diiodo-L-tyrosine.
The preparation of affinity sorbents containing immobilized iodinated derivatives of L-tyrosine for the affinity chromatography of porcine pepsin is described. The ligand was coupled either to Sepharose 4B or bead cellulose after the divinylsulfone activation or to Sepharose 4B after the activation with 2,4,6-trichloro-1,3,5-triazine. The highest capacity for porcine pepsin was found in the case of 3,5-diiodo-L-tyrosine coupled to divinylsulfone-activated Sepharose.
Arnostová
H
H
Department of Biochemistry, Charles University, Prague, Czech Republic.
Kucerová
Z
Z
Tislerová
I
I
Trnka
T
T
Tichá
M
M
D000818:Animals; D002846:Chromatography, Affinity / Q000379:methods*; D004105:Diiodotyrosine / Q000737:chemistry*; D013552:Swine; D014227:Triazines / Q000737:chemistry
D014227:Triazines; C019309:cyanuric chloride; D004105:Diiodotyrosine