Synthesis of structured lipids by transesterification of trilinolein catalyzed by Lipozyme IM60.
Structured lipids (SL) containing caprylic, stearic, and linoleic acids were synthesized by enzymatic transesterification using Lipozyme IM60. Pure trilinolein and free fatty acids were used as substrates. Incorporation of stearic acid was higher than that of caprylic acid in all parameters. Highest incorporations of both acids were achieved at 32 h, mole ratio of 1:4:4 (trilinolein/caprylic/stearic acids), water content of 1% (wt %), temperature of 55 degrees C, and 10% (wt %) enzyme load. The maximal incorporations of caprylic and stearic acids were 23.73 and 62.46 mol %, respectively. Reaction time, water content, and enzyme load had major influences on the reaction, whereas substrate mole ratio and temperature showed less influence. Lipozyme showed good stability over six reuses. Differential scanning calorimetric analysis of SL gave a melting profile with a very low melting peak of 0-3.3 degrees C and a solid fat content of 25.21% at 0 degrees C. The melting profile and solid fat content of SL were compared with those of fats extracted from commercially available solid and liquid margarine products. The data suggest that enzymatically produced SL could be used in liquid margarine products.
Sellappan
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Department of Food Science and Technology, The University of Georgia, Athens, Georgia 30602-7610, USA.
Akoh
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D002152:Calorimetry, Differential Scanning; D002384:Catalysis; D004951:Esterification; D005227:Fatty Acids / Q000032:analysis; D008049:Lipase / Q000378:metabolism*; D008055:Lipids / Q000138:chemical synthesis* / Q000737:chemistry; D010975:Platelet Aggregation Inhibitors / Q000138:chemical synthesis; D013696:Temperature; D014280:Triglycerides / Q000138:chemical synthesis*; D014867:Water / Q000032:analysis
D005227:Fatty Acids; D008055:Lipids; D010975:Platelet Aggregation Inhibitors; D014280:Triglycerides; D014867:Water; C093666:Lipozyme; D008049:Lipase; C009564:trilinolein