title: Expression of interleukin-8 in human metastatic endometrial carcinoma cells and its regulation by inflammatory cytokines.
abstract: In the present study, we analyzed the expression of a multifunctional cytokine, interleukin-8 (IL-8), in metastatic endometrial carcinoma cells. Our data demonstrate that human serous papillary endometrial adenocarcinoma (SPEC) and human endometrial adenocarcinoma (HEC) cells expressed steady-state IL-8-specific mRNA transcript and secreted IL-8 protein. The levels of IL-8 mRNA in SPEC-2 cells established from stage IV serous papillary adenocarcinoma were three-fold higher as compared to endometrial adenocarcinoma cells, HEC-1 A, established from stage IA endometrial cancer. Further, we observed higher levels of IL-8 mRNA and protein expression in the metastatic variants of SPEC-2 and HEC-1A cells as compared to the parent cell lines, demonstrating that IL-8 expression was associated with metastatic potential. Further, the treatment of endometrial carcinoma cells with inflammatory cytokines, IL-1beta and tumor necrosis factor-alpha (TNF-alpha), demonstrated that IL-1beta and TNF-alpha induced IL-8 expression in endometrial cancer cells. IL-1beta was a more potent inducer of IL-8 expression than TNF-alpha in our studies. These data demonstrate that constitutive and induced IL-8 expression in endometrial carcinoma cells might be an important regulatory mechanism of tumor growth and metastasis.
authors: Department of Cancer Biology, University of Texas, M. D. Anderson Cancer Center, Houston, Texas, USA.
authors: K K
authors: KK
authors: Berry
authors: M L
authors: ML
authors: Varney
authors: B J
authors: BJ
authors: Dave
authors: C D
authors: CD
authors: Bucana
authors: I J
authors: IJ
authors: Fidler
authors: R K
authors: RK
authors: Singh
mesh_terms: D002291:Carcinoma, Papillary / Q000473:pathology*; D016207:Cytokines / Q000494:pharmacology*; D016889:Endometrial Neoplasms / Q000473:pathology*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D016209:Interleukin-8 / Q000096:biosynthesis*; D009362:Neoplasm Metastasis / Q000503:physiopathology; D012333:RNA, Messenger / Q000032:analysis; D014407:Tumor Cells, Cultured
chemical_list: D016207:Cytokines; D016209:Interleukin-8; D012333:RNA, Messenger