Growth and differentiation of PC6 cells: the effects of pulsed electromagnetic fields (PEMF).
Previous studies in our laboratory showed that neurite outgrowth in vitro and nerve regeneration in vivo were stimulated by 2 Hz, 0.3 mT (3 G) pulsed electromagnetic fields (PEMF). To learn more about the effects of PEMF on nerve cells, we exposed PC6 cells, a standard neuronal-like cell model, to the same pulsed electromagnetic fields for 2 h/day for 2 days and asked whether two different cell processes, proliferation and differentiation, were affected. The cells were also treated with a differentiating agent, nerve growth factor (NGF), to further define any interactive effects. We found that proliferation was unaffected by either PEMF or NGF alone or in combination. Differentiation, expressed as neurite outgrowth, was strongly upregulated with NGF, but this NGF response was significantly depressed in cells treated with PEMF.
Shah
J P
JP
Center for Biomedical Engineering and Department of Anatomy and Neurobiology, University of Kentucky, Lexington 40506-0070, USA.
Midkiff
P
P
Brandt
P C
PC
Sisken
B F
BF
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects / Q000528:radiation effects*; D002455:Cell Division / Q000187:drug effects / Q000528:radiation effects*; D004574:Electromagnetic Fields; D007700:Kinetics; D008959:Models, Neurological; D020932:Nerve Growth Factor / Q000494:pharmacology; D009474:Neurons / Q000166:cytology* / Q000187:drug effects / Q000528:radiation effects; D016716:PC12 Cells; D051381:Rats; D013997:Time Factors
D020932:Nerve Growth Factor