Production of galactooligosaccharides from lactose using a beta-glucosidase from Thermus sp. Z-1.
A thermostable beta-glucosidase from Thermus sp. Z-1 that not only hydrolyzes beta-glucosides but also beta-galactosides was shown to efficiently produce oligosaccharides during hydrolysis of lactose. The yield of oligosaccharides was more than 40% for 0.88 M lactose solution at 70 degrees C at pH 7.0. The major product was a trisaccharide, 3'-galactosyllactose, formed by a galactosyltransfer reaction.
Akiyama
K
K
Graduate School of Practical Sciences, Showa Women's University, Tokyo, Japan. akiyama@swu.ac.jp
Takase
M
M
Horikoshi
K
K
Okonogi
S
S
D004795:Enzyme Stability; D004926:Escherichia coli / Q000235:genetics; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D007785:Lactose / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D009844:Oligosaccharides / Q000096:biosynthesis*; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D013379:Substrate Specificity; D013824:Thermus / Q000201:enzymology* / Q000235:genetics; D014312:Trisaccharides / Q000096:biosynthesis; D001617:beta-Glucosidase / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*
D009844:Oligosaccharides; D011994:Recombinant Proteins; D014312:Trisaccharides; C061732:3'-galactosyllactose; D001617:beta-Glucosidase; D007785:Lactose