Chromatographic study of highly methoxylated lime pectins deesterified by different pectin methyl-esterases.
The inter-molecular distribution of free carboxyl groups of two highly methoxylated pectins enzymatically deesterified by plant and fungus pectin methyl-esterases were investigated by size-exclusion (SEC) and ion-exchange chromatography (IEC). "Homogeneous" populations with respect to molar mass or charge density were thereby obtained and their chemical composition and physico-chemical properties (transport parameter for monovalent cations and calcium, calcium activity coefficient) were studied. Chemical analysis showed that the composition varies from one SEC fraction to another, the highest molar mass fraction being richer in rhamnose and galactose and exhibiting a slightly higher degree of methylation. Separation of pectins by IEC revealed a quite homogeneous charge density distribution for F58 contrary to P60 which exhibited a large distribution of methoxyl groups. The free carboxyl groups distributions and calcium binding behaviours of SEC and IEC fractions were shown to differ widely for highly methoxylated pectins deesterified by plant and fungus pectin methyl-esterases.
Ralet
M C
MC
Unité de Recherche sur les Polysaccharides, leurs Organisations et Interactions, Institut National de la Recherche Agronomique, Nantes, France. ralet@nantes.inra.fr
Bonnin
E
E
Thibault
J F
JF
D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D002412:Cations; D002850:Chromatography, Gel / Q000379:methods*; D002852:Chromatography, Ion Exchange / Q000379:methods*; D002957:Citrus / Q000737:chemistry*; D004951:Esterification; D010368:Pectins / Q000302:isolation & purification / Q000378:metabolism*
D002412:Cations; D010368:Pectins; D002265:Carboxylic Ester Hydrolases; C022323:pectinesterase