Fast separation of pyrimidine derivatives by capillary electrochromatography on ion-exchange/reversed-phase mixed-mode stationary phases.
This work describes the use of mixed-mode stationary phases which exhibit both strong ion-exchange (either cation-exchange, SCX, or anion-exchange, SAX) and reversed-phase chromatographic characteristics in capillary electrochromatographic separations of pyrimidine derivatives. Different packing materials, namely C6, SCX/C6 and SAX/C6, were compared and the influence of the composition of the carrier electrolyte (concentration of acetonitrile and pH) on the retention behavior of the selected solutes was investigated. A separation of all eight pyrimidine derivatives could be obtained on a 6.5 cm column packed with the SAX/C6 stationary phase in less than 3 min, with good peak shapes and efficiencies in the range 39,000 to 81,000 plates per meter.
Klampfl
C W
CW
Department of Analytical Chemistry, Johannes Kepler-University Linz, Austria. christian.klampfl@jk.uni-linz.ac.at
Buchberger
W
W
Haddad
P R
PR
D000097:Acetonitriles / Q000737:chemistry; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D004573:Electrolytes; D006863:Hydrogen-Ion Concentration; D011743:Pyrimidines / Q000302:isolation & purification*
D000097:Acetonitriles; D004573:Electrolytes; D011743:Pyrimidines; C032159:acetonitrile