[Intestinal flora of patients with suspected antibiotic associated diarrhea (AAD). I. Clostridium perfringens].
Stool samples of 158 patients suspected of antibiotic-associated diarrhoea (AAD) were studied. Toxin A of C. difficile and enterotoxin of C. perfringens were detected in stool samples by immunoenzymatic assays and PCR. In 35 stool samples toxin A of C. difficile was detected and in 48 cases (30%) C. difficile strains were cultured from 21 stool samples (13%). The presence of the cpe gene of C. perfringens, enabling the production of enterotoxin, could not be detected by PCR, both in stool samples and in isolated strains, using ent 1 and ent 2 primer pairs. C. difficile and C. perfringens were isolated from the same stool samples in 4 cases. From stool samples of two patients with AAD C. perfringens strains, thermoresistant spores were cultured.
Pituch
H
H
Katedra i Zakład Mikrobiologii Lekarskiej AM w Warszawie.
Martirosian
G
G
Obuch-Woszczatyński
P
P
Meisel-Mikołajczyk
F
F
Łuczak
M
M
D000900:Anti-Bacterial Agents / Q000009:adverse effects*; D016360:Clostridioides difficile / Q000302:isolation & purification; D003016:Clostridium perfringens / Q000302:isolation & purification; D003967:Diarrhea / Q000139:chemically induced* / Q000382:microbiology*; D004768:Enterotoxins / Q000032:analysis; D005243:Feces / Q000737:chemistry / Q000382:microbiology*; D006801:Humans
D000900:Anti-Bacterial Agents; D004768:Enterotoxins