Adenosine permeation of a dynamic in vitro blood-brain barrier inhibited by dipyridamole.
Adenosine is an inhibitory neuromodulator in the central nervous system and has been reported to have neuroprotective properties. Using a dynamic in vitro blood-brain barrier, we investigated the hypothesis that inhibition of adenosine transporters on the lumenal side of the blood-brain barrier may decrease the loss of adenosine from the brain. Our results indicate that lumenal administration of dipyridamole, a nucleoside transport inhibitor, can inhibit adenosine permeation from the extracapillary space into the lumen.
Sinclair
C J
CJ
Department of Pharmacology and Therapeutics, University of Manitoba, 753 McDermot Avenue, MB R3E 0T6, Winnipeg, Canada.
Krizanac-Bengez
L
L
Stanness
K A
KA
Janigro
D
D
Parkinson
F E
FE
D000241:Adenosine / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D000818:Animals; D001812:Blood-Brain Barrier / Q000502:physiology*; D002199:Capillary Permeability / Q000187:drug effects; D002352:Carrier Proteins / Q000037:antagonists & inhibitors; D002417:Cattle; D002478:Cells, Cultured; D004176:Dipyridamole / Q000494:pharmacology*; D008954:Models, Biological
D002352:Carrier Proteins; D004176:Dipyridamole; D000241:Adenosine