Delipidated serum abolishes the inhibitory effect of serum on in vitro liposome-mediated transfection.
All the standard in vitro lipofection has been routinely performed in serum-free medium as the transfection activity of liposome/DNA complexes is sensitive to the presence of serum. In this study, we have demonstrated that lipid-rich serum lipoprotein included in the transfection medium strongly inhibited the transfection activity of DC-chol liposome/DNA complexes in five different cell types (CHO, 293, A2780CP, A431 and SKBR3). The levels of inhibition by serum lipoprotein were rather greater than those by serum and varied with cell types. However, this inhibition was completely abolished by delipidation of serum. Thus, delipidated serum can be included in the transfection medium. The complexes formed in the presence of serum (zeta=-18.2+/-1.07 mV), delipidated serum (zeta=-19.6+/-0.54 mV), IgG (zeta=-21.6+/-1.92 mV) or serum lipoprotein (zeta=-10.5+/-2.33 mV) were as much negatively charged as those in serum-free medium (zeta=-21.3+/-1.60 mV). The results suggest that the inhibition of liposome-mediated transfection by serum was not associated with charges of serum proteins but with lipids or lipid-associated proteins present in serum.
Son
K K
KK
Department of Pharmaceutics, Rutgers, The State University of New Jersey, 160 Frelinghuysen Rd., 08854, Piscataway, NJ, USA. kson48@aol.com
Tkach
D
D
Rosenblatt
J
J
D000818:Animals; D001798:Blood Proteins; D016466:CHO Cells; D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D006224:Cricetinae; D006801:Humans; D007074:Immunoglobulin G; D008055:Lipids / Q000097:blood*; D008074:Lipoproteins; D008081:Liposomes; D010957:Plasmids; D014162:Transfection / Q000379:methods*; D014407:Tumor Cells, Cultured
D001798:Blood Proteins; D007074:Immunoglobulin G; D008055:Lipids; D008074:Lipoproteins; D008081:Liposomes; D015500:Chloramphenicol O-Acetyltransferase