title: Synonymous codon usage in Cryptosporidium parvum: identification of two distinct trends among genes.
abstract: The usage of alternative synonymous codons in the apicomplexan Cryptosporidium parvum has been investigated. A data set of 54 genes was analysed. Overall, A- and U-ending codons predominate, as expected in an A+T-rich genome. Two trends of codon usage variation among genes were identified using correspondence analysis. The primary trend is in the extent of usage of a subset of presumably translationally optimal codons, that are used at significantly higher frequencies in genes expected to be expressed at high levels. Fifteen of the 18 codons identified as optimal are more G+C-rich than the otherwise common codons, so that codon selection associated with translation opposes the general mutation bias. Among 40 genes with lower frequencies of these optimal codons, a secondary trend in G+C content was identified. In these genes, G+C content at synonymously variable third positions of codons is correlated with that in 5' and 3' flanking sequences, indicative of regional variation in G+C content, perhaps reflecting regional variation in mutational biases.
authors: Institute of Genetics, University of Nottingham, Queens Medical Centre, Nottingham, NG7 2UH, UK.
authors: R J
authors: RJ
authors: Grocock
authors: P M
authors: PM
authors: Sharp
mesh_terms: D000818:Animals; D003062:Codon / Q000737:chemistry / Q000235:genetics*; D016785:Cryptosporidium parvum / Q000737:chemistry / Q000235:genetics*; D015870:Gene Expression; D017125:Genes, Protozoan / Q000235:genetics*; D014644:Genetic Variation / Q000235:genetics
chemical_list: D003062:Codon