Calcium-induced changes in chondroitin sulfate chains of urinary trypsin inhibitor.
Urinary trypsin inhibitor (UTI) has several roles other than protease inhibition. It is suggested that UTI inhibits calcium influx in cultured cells and that the chondroitin sulfate chain of UTI may play an important role. In order to clarify the mechanistic features of this phenomenon, the chondroitin sulfate chain of UTI was analyzed by (1)H-NMR. The samples were highly purified UTI dissolved in D(2)O in the presence or absence of Ca(2+), Mg(2+) and Na(+). 1D-spectra were obtained and T(1) values of detected signals were estimated from the inversion-recovery method. The addition of Ca(2+) to UTI caused a chemical shift to downfield, line broadening and a reduction of T(1) values at several signals from chondroitin sulfate moiety (especially at axial H-2 of GalNAc), whereas Mg(2+) and Na(+) had no significant effect. Some of the signals in the linkage region of chondroitin sulfate chain showed marked line broadening by Ca(2+). The reduction of T(1) values implies formation of a complex. It is suggested that Ca(2+) generates the sulfate salt and interacts with other polar groups in the chondroitin sulfate chain, thereby causing bridging between UTI molecules. Several properties of UTI may be related to this interaction of Ca(2+) with chondroitin sulfate chains.
Masui
M
M
Department of Obstetrics and Gynecology, Hamamatsu University School of Medicine, 3600 Handa-cho, Hamamatsu 431-3192, Japan. Masui@sis.serei.or.jp
Suzuki
M
M
Fujise
Y
Y
Kanayama
N
N
D002118:Calcium / Q000378:metabolism* / Q000494:pharmacology; D002809:Chondroitin Sulfates / Q000737:chemistry* / Q000378:metabolism; D017666:Deuterium Oxide / Q000737:chemistry; D006023:Glycoproteins / Q000737:chemistry*; D046911:Macromolecular Substances; D008274:Magnesium / Q000378:metabolism / Q000494:pharmacology; D019906:Nuclear Magnetic Resonance, Biomolecular; D011485:Protein Binding / Q000187:drug effects; D012964:Sodium / Q000378:metabolism / Q000494:pharmacology
D006023:Glycoproteins; D046911:Macromolecular Substances; D002809:Chondroitin Sulfates; D012964:Sodium; D008274:Magnesium; D017666:Deuterium Oxide; C028665:urinastatin; D002118:Calcium