title: Human immune response to sand fly salivary gland antigens: a useful epidemiological marker?
abstract: Antibody (IgG) responses to salivary gland homogenate and to a recombinant salivary protein from the sand fly Lutzomyia longipalpis were investigated using sera from children living in an endemic area of visceral leishmaniasis in Brazil. We classified children into four groups according to their responses to Leishmania antigen: (Group I) positive serology and positive delayed type hypersensitivity (DTH), (Group II) positive serology and negative DTH, (Group III) negative serology and positive DTH, and (Group IV) negative serology and negative DTH. A highly significant correlation was found between anti-salivary gland IgG levels and DTH responses. An L. longipalpis salivary recombinant protein used as an antigen in an enzyme-linked immuno sorbent assay (ELISA) gave a significant but different result. A positive correlation was found between anti-Leishmania IgG and anti-recombinant protein IgG titers. The results indicate that sand fly salivary proteins may be of relevance to the study the epidemiology of leishmaniasis.
authors: Centro de Pesquisas Gonçalo Moniz (FIOCRUZ), Salvador, Bahia, Brazil. mbarral@cpqgm.fiocruz.br
authors: A
authors: A
authors: Barral
authors: E
authors: E
authors: Honda
authors: A
authors: A
authors: Caldas
authors: J
authors: J
authors: Costa
authors: V
authors: V
authors: Vinhas
authors: E D
authors: ED
authors: Rowton
authors: J G
authors: JG
authors: Valenzuela
authors: R
authors: R
authors: Charlab
authors: M
authors: M
authors: Barral-Netto
authors: J M
authors: JM
authors: Ribeiro
mesh_terms: D000818:Animals; D000906:Antibodies / Q000097:blood* / Q000276:immunology; D000941:Antigens / Q000276:immunology*; D015153:Blotting, Western; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D006968:Hypersensitivity, Delayed; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D007898:Leishmaniasis, Visceral / Q000453:epidemiology* / Q000276:immunology; D015800:Protozoan Proteins / Q000276:immunology; D011576:Psychodidae / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology; D012469:Salivary Glands / Q000276:immunology; D012471:Salivary Proteins and Peptides / Q000235:genetics / Q000276:immunology*
chemical_list: D000906:Antibodies; D000941:Antigens; D007074:Immunoglobulin G; D015800:Protozoan Proteins; D011994:Recombinant Proteins; D012471:Salivary Proteins and Peptides