HPLC evaluation of diclofenac in transdermal therapeutic preparations.
High-performance liquid chromatography (HPLC) was selected for analytical evaluation of sodium diclofenac in original transdermal therapeutic preparations containing adjuvant substances (capsaicin, hyoscyamine). After isolation from laminated adhesive patches, diclofenac was analysed on columns with reversed phase, using the mobile phase ethanol and phosphate buffer (pH 6.5) with an addition of tetrabutylammonium iodide and detection at 284 nm. Not only the total amount of diclofenac in the patch was evaluated, but HPLC methodology was also employed to select a suitable acceptor medium for permeation experiments. In patches manufactured in the tested series, HPLC was also employed to examine the release of diclofenac and its in vitro permeation through the human skin.
Klimes
J
J
Department of Pharmaceutical Chemistry and Drug Control, Faculty of Pharmacy, Charles University, Heyrovskeho 1203, 50005, Hradec Králové, Czech Republic. klimes@faf.cuni.cz
Sochor
J
J
Dolezal
P
P
Körner
J
J
D000269:Adhesives / Q000737:chemistry* / Q000493:pharmacokinetics; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000032:analysis* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid; D004008:Diclofenac / Q000032:analysis* / Q000493:pharmacokinetics; D015194:Diffusion Chambers, Culture; D006801:Humans; D012867:Skin / Q000378:metabolism
D000269:Adhesives; D000894:Anti-Inflammatory Agents, Non-Steroidal; D004008:Diclofenac