Regulation of longevity and stress resistance by Sch9 in yeast.
The protein kinase Akt/protein kinase B (PKB) is implicated in insulin signaling in mammals and functions in a pathway that regulates longevity and stress resistance in Caenorhabditis elegans. We screened for long-lived mutants in nondividing yeast Saccharomyces cerevisiae and identified mutations in adenylate cyclase and SCH9, which is homologous to Akt/PKB, that increase resistance to oxidants and extend life-span by up to threefold. Stress-resistance transcription factors Msn2/Msn4 and protein kinase Rim15 were required for this life-span extension. These results indicate that longevity is associated with increased investment in maintenance and show that highly conserved genes play similar roles in life-span regulation in S. cerevisiae and higher eukaryotes.
Fabrizio
P
P
Division of Biogerontology, Andrus Gerontology Center, and Department of Biological Sciences, University of Southern California, Los Angeles, CA 90089-0191, USA.
Pozza
F
F
Pletcher
S D
SD
Gendron
C M
CM
Longo
V D
VD
D000262:Adenylyl Cyclases / Q000235:genetics / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics / Q000502:physiology; D003470:Culture Media; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D004251:DNA Transposable Elements; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D004330:Drosophila / Q000235:genetics / Q000502:physiology; D004352:Drug Resistance, Microbial; D017353:Gene Deletion; D006358:Hot Temperature; D008136:Longevity; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D016877:Oxidants / Q000494:pharmacology; D010269:Paraquat / Q000494:pharmacology; D010641:Phenotype; D011494:Protein Kinases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins; D015398:Signal Transduction; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014170:Transformation, Genetic
D003470:Culture Media; D004251:DNA Transposable Elements; D004268:DNA-Binding Proteins; C081935:MSN2 protein, S cerevisiae; C081937:MSN4 protein, S cerevisiae; D016877:Oxidants; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D011494:Protein Kinases; C410736:Rim15 protein, S cerevisiae; C071569:SCH9 protein kinase; D017868:Cyclic AMP-Dependent Protein Kinases; D000262:Adenylyl Cyclases; D010269:Paraquat