title: Molecular cloning and functional analysis of pea cDNA E86 encoding homologous protein to hypersensitivity-related hsr203J.
abstract: Clone E86 was isolated as cDNA for elicitor-inducible gene from pea epicotyls by differential screening. The deduced amino acid sequence of E86 showed high homology to hypersensitivity-related protein hsr203J in tobacco and also showed significant homologies to the Ser-active hydrolases, such as mammalian hormone-sensitive lipases, bacterial lipases and esterases. E86 polypeptide possesses consensus amino acid sequence motifs (His-Gly) and (Gly-X-Ser-X-Gly) conserved in lipases and esterases and showed esterase degradation of p-nitrophenyl butyrate. Northern blot analysis revealed that the E86-transcript is abundant in roots and stems and was induced by fungal elicitor in pea epicotyls. However, elicitor-induced accumulation of E86 mRNA was significantly inhibited by the fungal suppressor. Furthermore the expression of the genes encoding E86 and phenylalanine ammonia-lyase was induced within 1 h after the inoculation of a nonpathogen, but it was delayed for 5 h by the inoculation of a compatible pathogen. These results suggest that the elicitor-induced Ser-active hydrolase derived from E86 gene might be related to the plant defense responses.
authors: Laboratory of Plant Pathology and Genetic Engineering, Graduate School of Natural Science and Technology, Okayama University, 3-1-1, Tsushima-naka, 700-8530, Okayama, Japan
authors: Y
authors: Y
authors: Ichinose
authors: Y
authors: Y
authors: Hisayasu
authors: S
authors: S
authors: Sanematsu
authors: Y
authors: Y
authors: Ishiga
authors: H
authors: H
authors: Seki
authors: K
authors: K
authors: Toyoda
authors: T
authors: T
authors: Shiraishi
authors: T
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authors: Yamada