Physical interaction of human papillomavirus virus-like particles with immune cells.
Human papillomavirus virus-like particles (HPV VLP) and chimeric VLP are immunogens that are able to elicit potent anti-viral/tumor B and T cell responses. To investigate the immunogenicity of VLP, we determined which cells of the immune system are able to bind HPV-16 VLP. VLP were found to bind very well to human and mouse immune cells that expressed markers of antigen-presenting cells (APC) such as MHC class II, CD80 and CD86, including dendritic cells, macrophages and B cells. mAb blocking studies identified Fc gamma RIII (CD16) as one of the molecules to which the VLP can bind both on immune cells and foreskin epithelium. However, transfection of a CD16(-) cell line with CD16 did not confer binding of VLP. Splenocytes from Fc gamma RIII knockout mice showed a 33% decrease in VLP binding overall and specifically to subsets of APC. These combined data support a role for CD16 as an accessory molecule in an HPV VLP-receptor complex, possibly contributing to the immunogenicity of HPV VLP.
Da Silva
D M
DM
Cancer Immunology Program, Cardinal Bernardin Cancer Center and Department of Microbiology & Immunology, Loyola University Chicago, 2160 South First Avenue, Maywood, IL 60153, USA.
Velders
M P
MP
Nieland
J D
JD
Schiller
J T
JT
Nickoloff
B J
BJ
Kast
W M
WM
D000818:Animals; D019138:Antibodies, Blocking; D000938:Antigen-Presenting Cells / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology; D001483:Base Sequence; D002460:Cell Line; D002678:Chimera / Q000276:immunology; D017931:DNA Primers / Q000235:genetics; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D027383:Papillomaviridae / Q000276:immunology* / Q000472:pathogenicity; D030361:Papillomavirus Infections / Q000276:immunology; D017452:Receptors, IgG / Q000235:genetics / Q000378:metabolism; D014162:Transfection; D014412:Tumor Virus Infections / Q000276:immunology
D019138:Antibodies, Blocking; D017931:DNA Primers; D017452:Receptors, IgG