Use of lipophilic anions for estimation of biomass and cell viability.
A method is described to estimate the microbial biomass of a sample, to enumerate the cells, and to distinguish the portion of metabolically active cells in the population by measuring the binding of phenyldicarbaundecaborane (PCB(-)) to the cells. This method can also be used for the analysis of a complex population of microorganisms if the cells composing the sample are sensitive to different biocidal agents. In addition, the analysis of PCB(-) binding is useful for the enumeration of the phage-infected cells and phage particles.
Daugelavicius
R
R
Department of Biochemistry and Biophysics, Vilnius University, Ciurlionio 21, LT-2009 Vilnius, Lithuania. Rimantas.Daugelavicius@gf.vu.lt
Bakiene
E
E
Berzinskiene
J
J
Bamford
D H
DH
D001435:Bacteriophages / Q000187:drug effects / Q000502:physiology*; D018533:Biomass; D001896:Boron Compounds / Q000737:chemistry; D002470:Cell Survival / Q000187:drug effects*; D004926:Escherichia coli / Q000187:drug effects / Q000502:physiology*; D007700:Kinetics; D008962:Models, Theoretical; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000502:physiology*; D013057:Spectrum Analysis / Q000379:methods*
D001896:Boron Compounds; C100079:phenyldicarbaundecaborane