Detection of Salmonella enteritidis in shell and liquid eggs using enrichment and plating.
Detection methods using various enrichment and plating media and immunoconcentration for Salmonella enteritidis in shell and liquid eggs were evaluated. For liquid egg samples naturally contaminated with S. enteritidis, pre-enrichment in 225 ml of buffered peptone water with cysteine followed by selective enrichment in 10 ml of tetrathionate broth was the superior, resulting in the detection of S. enteritidis in all samples on six of the seven types of selective agar substrate investigated. This enrichment procedure also enabled detection of S. enteritidis in most of artificially inoculated shell egg and pasteurized liquid egg samples.
Hara-Kudo
Y
Y
Department of Biomedical Food Research, National Institute of Infectious Diseases, Tokyo, Japan.
Kumagai
S
S
Masuda
T
T
Goto
K
K
Ohtsuka
K
K
Masaki
H
H
Tanaka
H
H
Tanno
K
K
Miyahara
M
M
Konuma
H
H
D000818:Animals; D015169:Colony Count, Microbial; D003470:Culture Media; D004528:Egg Shell / Q000382:microbiology; D004531:Eggs / Q000382:microbiology*; D018189:Immunomagnetic Separation; D012477:Salmonella enteritidis / Q000302:isolation & purification*
D003470:Culture Media