title: Composition strand asymmetries in prokaryotic genomes: mutational bias and biased gene orientation.
abstract: Most prokaryotic genomes display strand compositional asymmetries, but the reasons for these biases remain unclear. When the distribution of gene orientation is biased, as it often is, this may induce a bias in composition, as codon frequencies are not identical. We show here that this effect can be estimated and removed, and that the residual base skews are the highest at third base codon positions and lower at first and second positions. This strongly suggests that compositional asymmetries result from 1) a replication-related mutational bias that is filtered through selective pressure and/or from 2) an uneven distribution of gene orientation. In most cases, the mutational bias alters the codon usage and amino acid frequencies of the leading and the lagging strand. However, these features are not ubiquitous amongst prokaryotes, and the biological reasons for them remain to be found.
authors: Equipe phylogénie, bio-informatique et génome, UMR 7622, bâtiment B, 6e étage, case 24, 9, quai Saint-Bernard, 75252 Paris, France.
authors: P
authors: P
authors: Lopez
authors: H
authors: H
authors: Philippe
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics; D001419:Bacteria / Q000235:genetics*; D015748:Borrelia burgdorferi Group / Q000235:genetics; D016680:Genome, Bacterial; D008957:Models, Genetic; D009154:Mutation; D009169:Mycobacterium tuberculosis / Q000235:genetics; D011387:Prokaryotic Cells; D014158:Transcription, Genetic