Electroporation of the skin to deliver antigen by using a piezo ceramic gas igniter.
The static electricity generated by triggering a piezo gas igniter is shown to cause electroporation of the skin to deliver antigen. Mice were immunized with chicken albumin by electroporation using a piezo gas igniter and in another experiment, the gas igniter was replaced by a power supply. In both the groups identical immune responses were generated. The change in impedance of the skin of a mice after applying high voltage electrical pulses from a power supply and that with a gas igniter were found to be similar. The piezo gas igniter is an inexpensive and easily available device. It is much more user 'friendly' than a power supply used for electroporation and it may be viewed as a replacement for a syringe with a needle.
Upadhyay
P
P
National Institute of Immunology, Aruna Asaf Ali Marg, 110067, New Delhi, India. pkumar@nii.res.in
D000418:Albumins / Q000008:administration & dosage* / Q000276:immunology; D000818:Animals; D000941:Antigens / Q000008:administration & dosage* / Q000276:immunology; D002645:Chickens; D016503:Drug Delivery Systems / Q000295:instrumentation* / Q000379:methods; D004558:Electric Stimulation / Q000295:instrumentation / Q000379:methods; D018274:Electroporation / Q000295:instrumentation* / Q000379:methods; D005712:Galvanic Skin Response / Q000187:drug effects / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D012867:Skin / Q000187:drug effects* / Q000276:immunology; D055672:Static Electricity
D000418:Albumins; D000941:Antigens