Male accessory gland derived factors can stimulate oogenesis and enhance oviposition in Helicoverpa armigera (Lepidoptera: Noctuidae).
In Helicoverpa armigera, female moths began to lay eggs on the third day after emergence. Mating stimulated earlier egg maturation/oogenesis (P = 0.002) and oviposition (P << 0.01). We established a suitable bioassay model for the influence of male accessory glands (MAG) on the physiology of virgin females: Crude extracts of MAG (2- to 3-day-old) were injected into 2-day-old virgin females, and the injected females were dissected 20 h after mating. It was shown that crude extracts of MAG stimulated earlier egg maturation (P < 0.001) and oviposition (the oviposition ratio was more than 2 times the ratio of the control). Proteinaceous components in crude extracts purified by fractionation and sub-fractionation in reverse phase high performance liquid chromatography also stimulated earlier egg maturation (P < 0.01) and ovipositon (more than 2 times the ratio of the control), and we called them the oogenesis and ovipostion factors (OOSF). With SDS-PAGE, the molecular mass of the bands from OOSF was estimated to be between 55-66 KD. Arch.
Jin
Z Y
ZY
State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Academia Sinica, Beijing, China.
Gong
H
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D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005837:Genitalia, Male / Q000378:metabolism / Q000502:physiology; D019476:Insect Proteins / Q000378:metabolism; D008297:Male; D009036:Moths / Q000502:physiology*; D009866:Oogenesis / Q000502:physiology*; D010058:Oviposition / Q000502:physiology*; D012727:Sex Characteristics; D012726:Sexual Behavior, Animal
D019476:Insect Proteins