title: A preliminary study of the population genetics of Aedes aegypti (Diptera: Culicidae) from Mexico using microsatellite and AFLP markers.
abstract: Dengue fever recently reemerged in the Americas. Because vaccines are still under development, dengue prevention depends entirely on vector control. Since Aedes aegypti (Linnaeus, 1762) is the principal vector of this arbovirus, knowledge of the genetic structure of the insect is therefore required to maintain effective vector control strategies and to estimate levels of gene flow from which movement can be inferred. This preliminary study uses microsatellite and amplified fragment length polymorphism (AFLP) markers, to provide insights into genetic diversity of A. aegypti populations from different districts of two towns, located in the north-west of Mexico, Hermosillo and Guaymas. Although the microsatellites used were found to display limited polymorphism, they allowed discrimination between mosquitoes from the northern and the southern districts of Hermosillo. Using AFLP markers, clustering of individuals from the same town and from the same district was observed. Data from microsatellite and AFLP markers analysis both suggest that reinvasion of A. aegypti probably occurs from Guaymas to Hermosillo.
authors: Laboratoire de Recherche et de Coordination sur les Trypanosomoses, IRD-CIRAD, Programme Santé Animale, TA/30G, Campus International de Baillarguet, 34398 Montpellier Cedex 5, France. ravel@mpl.ird.fr
authors: S
authors: S
authors: Ravel
authors: N
authors: N
authors: Monteny
authors: D
authors: D
authors: Velasco Olmos
authors: J
authors: J
authors: Escalante Verdugo
authors: G
authors: G
authors: Cuny
mesh_terms: D000330:Aedes / Q000737:chemistry / Q000235:genetics*; D000483:Alleles; D000818:Animals; D004247:DNA / Q000737:chemistry; D003715:Dengue / Q000517:prevention & control / Q000635:transmission; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D014644:Genetic Variation / Q000235:genetics; D005828:Genetics, Population; D008800:Mexico; D018895:Microsatellite Repeats / Q000235:genetics*; D010802:Phylogeny; D010865:Pilot Projects; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic
chemical_list: D004247:DNA