In vitro pituitary prolactin, growth hormone and follicle stimulating hormone secretion during sexual maturation of female rats primed with melatonin.
The influence of in vivo melatonin administration on in vitro pituitary follicle stimulating hormone (FSH), growth hormone (GH) and prolactin secretion, as well as the possible influence of dopamine (DA) were evaluated in prepubertal (31-day-old), pubertal (33-day-old) and adult female rats at diestrus phase of the sexual cycle. The in vitro pituitary hormone secretions were evaluated at basal rate for the first hour of incubation only, in Krebs Ringer phosphate (KRP) (I1) and after a second hour of incubation with KRP (I2) or with KRP+DA (I2 plus DA). I1PRL secretion was significantly higher in 33-day-old control and melatonin treated (MEL) rats as compared to I2 periods. However, in 31-day-old rats I1 secretion was higher than in the I2 or I2+DA periods, in MEL rats. In vitro GH secretion was significantly higher at I1 than during I2 periods in the control 31- and 33-day-old groups, but not in MEL rats. The only significant effect of DA was the elevation of GH in prepubertal MEL rats. In vitro FSH release was increased by melatonin in 31- and 33-day-old female rats. No differences in PRL, GH and FSH secretion were found in adult rats. In conclusion, the results show that melatonin effects upon in vitro pituitary gland activity are reproductive-stage-dependent modifying the secretory capacity of the lactotrop, gonadotrop and somatotrop during prepubertal and pubertal ages but not in adult rats studied at a quiescent phase of the sexual cycle.
Díaz Rodríguez
E
E
Department of Functional Biology, Area of Physiology, Faculty of Medicine, University of Oviedo, Julian Clavería, 6, 33006 Oviedo, Spain.
Fernández Alvarez
C
C
Castrillón
P O
PO
Esquifino Parras
A I
AI
Díaz López
B
B
D000818:Animals; D004298:Dopamine / Q000502:physiology; D005260:Female; D005640:Follicle Stimulating Hormone / Q000378:metabolism*; D019382:Human Growth Hormone / Q000378:metabolism*; D066298:In Vitro Techniques; D008550:Melatonin / Q000494:pharmacology*; D011388:Prolactin / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012741:Sexual Maturation / Q000502:physiology*; D013268:Stimulation, Chemical
D019382:Human Growth Hormone; D011388:Prolactin; D005640:Follicle Stimulating Hormone; D008550:Melatonin; D004298:Dopamine