[The influence of interleukin-1 on nerve growth factor secretion in newborn rat astrocytes in vitro].
OBJECTIVE
To study the nerve growth factor (NGF) expression and the influence of IL-1 alpha or IL-1 beta on NGF secretion in newborn rat astrocytes.


METHODS
Astrocytes obtained from the brain cortex of newborn rats were cultured and purified, and they were divided into three groups, experimental, control and blank groups. IL-1 alpha or IL-1 beta were added into the experimental group with 25, 50 and 100 U/ml, each group was cultured for 24, 48 or 72 hours, and then the NGF contents in cultured astrocytes suspension media were measured by a two-cite enzymelinked immunoserbent assay (ELISA).


RESULTS
Astrocytes could secret NGF by themselves and each concentration of IL-1 alpha or IL-1 beta media at any testing time could enhance NGF secreting in newborn rat astrocytes in certain degrees. The effects of IL-1 beta were stronger than IL-1 alpha, the strongest effect in the unit time was observed in IL-1 beta with 50 U/ml for 24 hours.


CONCLUSIONS
Astrocytes can express NGF, and IL-1 alpha or IL-1 beta can enhance the NGF expression in newborn rat astrocytes.
Guo
J
J
157th Hospital of PLA, Guangzhou Guangdong, P. R. China 510510.
Ma
X Y
XY
Zeng
J P
JP
D000818:Animals; D000831:Animals, Newborn; D001253:Astrocytes / Q000187:drug effects / Q000378:metabolism*; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000494:pharmacology*; D020932:Nerve Growth Factor / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
D007375:Interleukin-1; D020932:Nerve Growth Factor