Survivin expression correlates with apoptosis resistance after lymphocyte activation and is found preferentially in memory T cells.
The prevention of apoptosis may be critical for immunological function. Survivin is a recently cloned member of the inhibitor of apoptosis protein family. We analyzed survivin expression before and after lymphocyte activation in isolated cell populations. Prior to activation, survivin was undetectable. After activation with IL-2 and OKT-3, CD3(+) cells expressed survivin. Next, we correlated survivin expression with Fas, FasL and the amount of apoptosis over time in culture. After activation, survivin was readily detected by day 2 and decreased thereafter. Prior to activation (day 0), Fas was present on 60% of the cells and on 100% by days 2-6. Peak FasL mRNA expression was at day 2. During peak survivin expression (days 2-4) the apoptotic fraction was low, but when survivin expression decreased the apoptotic fraction increased rapidly. Finally, we found that CD45RO(+) memory T cells showed a higher expression of survivin than did CD45RA(+) naive T cells after activation. These results suggest that survivin may contribute to T-cell survival early in T-cell responses as well as in memory immune responses.
Kornacker
M
M
Division of Bone Marrow Transplantation, Stanford University Medical Center, 300 Pasteur Drive, Stanford, CA 94305, USA.
Verneris
M R
MR
Kornacker
B
B
Scheffold
C
C
Negrin
R S
RS
D017209:Apoptosis / Q000276:immunology*; D015870:Gene Expression; D006801:Humans; D007156:Immunologic Memory / Q000276:immunology*; D051034:Inhibitor of Apoptosis Proteins; D008213:Lymphocyte Activation / Q000276:immunology*; D008869:Microtubule-Associated Proteins; D009363:Neoplasm Proteins; D011506:Proteins / Q000235:genetics*; D000077022:Survivin; D013601:T-Lymphocytes / Q000276:immunology*; D013997:Time Factors
C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins; D009363:Neoplasm Proteins; D011506:Proteins; D000077022:Survivin