Detection of interleukin-8 in exudates from normal and inflamed human dental pulp tissues.
OBJECTIVE
The purpose of this study was to investigate the level of IL-8 in exudates clinically obtained from normal and inflamed human dental pulp tissues so as to reveal the possible relationship between IL-8 and pulpitis.


METHODS
Samples of 2 microliters of pulpal exudate from each normal or clinically diagnosed as acute or chronic pulpitis teeth was obtained by filter paper strips and IL-8 level was measured by ELISA method.


RESULTS
No IL-8 was detected in the samples from normal pulp, but significant amount of IL-8 appeared in inflamed pulp tissues, and the level of IL-8 in exudates of acute stage of pulpitis was higher than that of chronic stage (P < 0.01).


CONCLUSIONS
This study demonstrates that IL-8 is produced and accumulated in pulp inflammation and may play a role in the occurrence and development of human pulpitis.
Guo
X
X
Department of Endodontics, School of Stomatology, Fourth Military Medical University, No. 17 ChangLe Xi Road, Xincheng District, Xi'an, Shaanxi, 710032, P. R. China.
Niu
Z
Z
Xiao
M
M
Yue
L
L
Lu
H
H
D000208:Acute Disease; D002908:Chronic Disease; D003782:Dental Pulp / Q000737:chemistry / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005122:Exudates and Transudates; D006801:Humans; D018836:Inflammation Mediators / Q000032:analysis; D016209:Interleukin-8 / Q000032:analysis*; D008747:Methylcellulose; D011671:Pulpitis / Q000276:immunology* / Q000378:metabolism
D018836:Inflammation Mediators; D016209:Interleukin-8; D008747:Methylcellulose