Understanding and modulating apoptosis in industrial cell culture.
Currently, the production of therapeutic recombinant proteins relies heavily on the large-scale culture of eukaryotic cells that secrete the protein of interest into the media. It has been recognized that programmed cell death, or apoptosis, may pose a significant hurdle to maximum productivity in such systems. With a greater understanding of the molecular events causing apoptosis, alterations can be made to the cells and culture conditions to prevent apoptosis and enhance volumetric productivity.
Laken
H A
HA
Genetics Institute/Wyeth-Ayerst Research, Cell and Molecular Sciences, 1 Burtt Road, Andover, MA 01810, USA. hlaken@genetics.com
Leonard
M W
MW
D017209:Apoptosis / Q000502:physiology*; D018929:Cell Culture Techniques / Q000379:methods*; D004345:Drug Industry; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis / Q000502:physiology*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics
D019253:Proto-Oncogene Proteins c-bcl-2; D011994:Recombinant Proteins