title: Near-infrared spectrometric determination of di- and tripeptides synthesized by a combinatorial solid-phase method.
abstract: A new method based on near-infrared (NIR) spectrometry and partial least-squares analysis has been developed for the noninvasive and nondestructive determination of the identity and sequences of amino acid residues in di- and tripeptides. The di- and tripeptides were synthesized from six amino acids with similar structures (Gly, Ala, Leu, Met, Phe, Val) on two different polymer beads (bead with and without a linker) using the solid-phase peptide synthetic method. The developed NIR method is capable of determining the identity of sequences of these di- and tripeptides (with and without the Fmoc protecting group) directly on the polymer beads. It can distinguish not only dipeptides from tripeptides but also peptides with very similar structures (e.g., bead-Gly-Ala-Ala, bead-Gly-Ala-Phe, bead-Gly-Ala-Leu, bead-Gly-Ala-Val, and bead-Gly-Ala-Met). More importantly, the method is capable of distinguishing di- and tripeptides with the same amino acid residues but different sequences (e.g., bead-Gly-Leu-Val from bead-Gly-Val-Leu).
authors: Department of Chemistry, Marquette University, Milwaukee, Wisconsin 53201, USA.
authors: T
authors: T
authors: Alexander
authors: C D
authors: CD
authors: Tran
mesh_terms: D020650:Combinatorial Chemistry Techniques; D004151:Dipeptides / Q000032:analysis* / Q000138:chemical synthesis; D007202:Indicators and Reagents; D009842:Oligopeptides / Q000032:analysis* / Q000138:chemical synthesis; D019265:Spectroscopy, Near-Infrared
chemical_list: D004151:Dipeptides; D007202:Indicators and Reagents; D009842:Oligopeptides