Measurement of canine IgE using the alpha chain of the human high affinity IgE receptor.
In vitro assays for allergen specific immunoglobulin E (IgE) are a convenient and reproducible alternative to intradermal skin testing in dogs. Such tests may be used to support a diagnosis of atopic dermatitis and to define appropriate allergens for immunotherapy. Current in vitro assays rely upon monoclonal or polyclonal antibodies as IgE detection reagents. However, in sera where allergen-specific IgG occurs in great excess, any IgE:IgG cross-reactivity of the detection reagent may result in lowered assay specificity. Therefore, we have developed an assay for canine IgE which uses a recombinant form of the extracellular part of the alpha chain of the human high affinity IgE receptor (FcvarepsilonRIalpha). Biotinylated FcvarepsilonRIalpha shows no significant binding to purified canine IgG, and recognizes a heat labile antibody in serum, with a detection limit of 73-146pg/ml. Comparison of assay signals using the labeled FcvarepsilonRIalpha and a highly specific anti-canine IgE monoclonal antibody (MAb) shows good agreement. The FcvarepsilonRIalpha is therefore a sensitive and specific alternative to polyclonal or monoclonal antibodies for canine serum IgE measurement.
Stedman
K
K
Heska Corporation, 1613 Prospect Parkway, Fort Collins, CO 80525, USA. mccallc@heska.com
Lee
K
K
Hunter
S
S
Rivoire
B
B
McCall
C
C
Wassom
D
D
D000818:Animals; D000911:Antibodies, Monoclonal; D004285:Dogs / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D006801:Humans; D007073:Immunoglobulin E / Q000032:analysis* / Q000097:blood; D007137:Immunoglobulin alpha-Chains / Q000032:analysis*; D011487:Protein Conformation; D017455:Receptors, IgE / Q000737:chemistry*
D000911:Antibodies, Monoclonal; D007137:Immunoglobulin alpha-Chains; D017455:Receptors, IgE; D007073:Immunoglobulin E