title: Characterization of the inflammatory infiltrate during IgE-mediated late phase reactions in the skin of normal and atopic dogs.
abstract: In canine and human atopic patients, the intracutaneous injection of offending allergens is followed by the development of both immediate and late-phase reactions. The present study was performed to expand on the characterization and dynamics of inflammatory cell subsets during IgE-mediated late-phase reactions in canine skin. Three normal dogs and three Dermatophagoides farinae-allergic dogs were selected for this experiment. All dogs were challenged intradermally with mite allergen, purified anticanine IgE antibodies (positive control) or phosphate-buffered saline (negative control). Skin biopsies were obtained before and 6, 12 and 24 h post-injection. Sections were stained with metachromatic and eosinophil-specific histological stains. Additionally, we used an immunohistochemical method with antibodies specific for canine leukocyte antigens. This study confirmed the occurrence of a late-phase reaction in atopic skin following allergen challenge, and in normal and atopic canine skin after intradermal injection of IgE-specific antibodies. Whereas early emigrating dermal cells were composed chiefly of neutrophil and activated eosinophil granulocytes, there was an influx of alpha beta T-lymphocytes and dermal dendritic cells in later stages of the late-phase reactions. Because IgE-mediated late-phase reactions resemble spontaneous atopic canine skin lesions, both at macroscopic and microscopic levels, we propose the use of similar challenges to study the anti-inflammatory effects of anti-allergic drugs in a pre-clinical setting.
authors: Department of Clinical Sciences, North Carolina State University, College of Veterinary Medicine, 4700 Hillsborough Street, Raleigh, NC 27606, USA.
authors: T
authors: T
authors: Olivry
authors: S M
authors: SM
authors: Dunston
authors: K M
authors: KM
authors: Murphy
authors: P F
authors: PF
authors: Moore
mesh_terms: D000485:Allergens; D000818:Animals; D016022:Case-Control Studies; D003876:Dermatitis, Atopic / Q000276:immunology / Q000662:veterinary*; D004283:Dog Diseases / Q000276:immunology*; D004285:Dogs; D007073:Immunoglobulin E / Q000276:immunology*; D007150:Immunohistochemistry / Q000662:veterinary; D007962:Leukocytes; D008925:Mites
chemical_list: D000485:Allergens; D007073:Immunoglobulin E