[Cloning the differentially expressed genes in the retina of rds mouse during the development of retinitis pigmentosa].
OBJECTIVE
To clone the differentially expressed genes in the retina of rds mouse (the animal model of congenital retinitis pigmentosa) during the disease development.


METHODS
The retinal mRNA of rds mouse during the development of retinitis pigmentosa was analyzed by the mRNA differential display. The differentially expressed mRNA fragments were cloned and sequenced.


RESULTS
There was obvious difference of gene expression between rds mouse and the control during the development of retinitis pigmentosa. Five differentially expressed bands were cloned and sequenced. One of those had 86% identity (132/154) with the sequence of the human cDNA DKFZp434D1227 from adult testis in GenBank, which was submitted lately (15-Oct-1999) and without much information. The other had lower identity with the sequences in GenBank. A highly expressed clone in the rds mouse on postnatal day 25 had the same length as another clone in the normal on postnatal day 37, which was not expressed in the rds mouse on day 37. The sequences of the two clones were identical in all but two base pairs.


CONCLUSIONS
These results indicate that there are a lot of novel differentially expressed genes in the chronic processing diseases, such as retinitis pigmentosa.
Shen
H
H
Zhongshan Ophthalmic Center, Sun Yat-Sen University of Medical Sciences, Guangzhou, Guangdong 510060 P. R. China. ZOCZGJ@gzsums.edu.cn
Zhang
Q
Q
Kuang
Z
Z
Xiao
X
X
Li
R
R
D000818:Animals; D003001:Cloning, Molecular; D004195:Disease Models, Animal; D020869:Gene Expression Profiling; D007381:Intermediate Filament Proteins / Q000235:genetics*; D008562:Membrane Glycoproteins; D051379:Mice; D008809:Mice, Inbred C3H; D009419:Nerve Tissue Proteins / Q000235:genetics*; D064531:Peripherins; D012160:Retina / Q000378:metabolism*; D012174:Retinitis Pigmentosa / Q000235:genetics*
D007381:Intermediate Filament Proteins; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; C578997:PRPH2 protein, human; D064531:Peripherins; C578996:Prph2 protein, mouse