title: Ontogeny, distribution and function of CD38-expressing B lymphocytes in mice.
abstract: Analysis of expression of CD38, CD45R (B220), IgM and IgD on splenic B lymphocytes from mice of different ages demonstrated CD38 on both immature (B220(+), BCR(-)) and mature (B220(+), BCR(+)) B lymphocytes. Similarly, CD38 is expressed as early as B220 on the surface of progenitor B cells in the bone marrow. In spite of expressing of CD38 and IgM, neonatal B cells, in contrast to the adult, failed to proliferate to either anti-CD38 or anti-IgM cross-linking when IL-4 was present. They did, however, respond to LPS and anti-CD40, and by 2 weeks of age they began to respond to anti-CD38 and anti-IgM, reaching adult B cell levels by 4 weeks. Although the distribution of CD38 on adult B cells from most different lymphoid compartments was broadly similar, significantly higher levels of CD38 were expressed on peritoneal B lymphocytes. A detailed analysis, using IgM / IgD ratio and staining with anti-CD5 confirmed that B1 lymphocytes were expressing a high level of CD38. Interestingly, both immature B cells and peritoneal B1 lymphocytes were unresponsive to anti-CD38. However, they were activated by LPS or anti-CD40.
authors: Department of Molecular Biomedicine, Centro de Investigación y Estudios Avanzados del I.P.N., México D. F., México.
authors: F R
authors: FR
authors: Donís-Hernández
authors: R M
authors: RM
authors: Parkhouse
authors: L
authors: L
authors: Santos-Argumedo
mesh_terms: D036541:ADP-ribosyl Cyclase; D051997:ADP-ribosyl Cyclase 1; D000375:Aging / Q000276:immunology; D000818:Animals; D000906:Antibodies; D015703:Antigens, CD; D000943:Antigens, Differentiation / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000378:metabolism*; D001854:Bone Marrow Cells / Q000276:immunology / Q000378:metabolism; D019013:CD40 Antigens / Q000276:immunology; D002454:Cell Differentiation; D002455:Cell Division; D005260:Female; D005434:Flow Cytometry; D007072:Immunoglobulin D / Q000032:analysis / Q000276:immunology; D007075:Immunoglobulin M / Q000032:analysis / Q000276:immunology; D008070:Lipopolysaccharides / Q000276:immunology; D008213:Lymphocyte Activation; D008562:Membrane Glycoproteins; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D009244:NAD+ Nucleosidase / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D010529:Peritoneal Cavity / Q000166:cytology; D010581:Peyer's Patches / Q000276:immunology; D011940:Receptor Aggregation; D013154:Spleen / Q000276:immunology; D013234:Stem Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism
chemical_list: D000906:Antibodies; D015703:Antigens, CD; D000943:Antigens, Differentiation; D019013:CD40 Antigens; D007072:Immunoglobulin D; D007075:Immunoglobulin M; D008070:Lipopolysaccharides; D008562:Membrane Glycoproteins; D036541:ADP-ribosyl Cyclase; C498155:Cd38 protein, mouse; D009244:NAD+ Nucleosidase; D051997:ADP-ribosyl Cyclase 1