Isolation and characterization of a cDNA encoding a novel member of the human regenerating protein family: Reg IV.
Human Reg and Reg-related genes constitute a multi-gene family belonging to the calcium (C-type) dependent lectin superfamily. Regenerating gene family members are expressed in the proximal gastrointestinal (GI) tract and ectopically at other sites in the setting of tissue injury. By high-throughput sequence analysis of a large inflammatory bowel disease library, two cDNAs have been isolated which encode a novel member of this multigene family. Based on primary sequence homology, tissue expression profiles, and shared exon-intron junction genomic organization, we assign this gene to the regenerating gene family. Specific protein structural differences suggest that the current three regenerating gene subtypes should be expanded to four. We demonstrate that Reg IV has a highly restricted tissue expression pattern, with prominent expression in the gastrointestinal tract. Reg IV mRNA expression is significantly up-regulated by mucosal injury from active Crohn's disease or ulcerative colitis.
Hartupee
J C
JC
Division of Gastroenterology, Washington University School of Medicine, 660 S. Euclid Ave., St. Louis, MO 63110, USA.
Zhang
H
H
Bonaldo
M F
MF
Soares
M B
MB
Dieckgraefe
B K
BK
D000595:Amino Acid Sequence; D001483:Base Sequence; D002135:Calcium-Binding Proteins / Q000737:chemistry / Q000235:genetics*; D018076:DNA, Complementary / Q000737:chemistry / Q000302:isolation & purification; D020224:Expressed Sequence Tags; D006801:Humans; D015212:Inflammatory Bowel Diseases / Q000235:genetics; D007413:Intestinal Mucosa / Q000378:metabolism; D037102:Lectins / Q000737:chemistry / Q000235:genetics*; D037181:Lectins, C-Type; D051847:Lithostathine; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins; D000073718:Pancreatitis-Associated Proteins; D012333:RNA, Messenger / Q000032:analysis; D016415:Sequence Alignment
D002135:Calcium-Binding Proteins; D018076:DNA, Complementary; D037102:Lectins; D037181:Lectins, C-Type; D051847:Lithostathine; D009419:Nerve Tissue Proteins; D000073718:Pancreatitis-Associated Proteins; C497617:REG1A protein, human; C428528:REG4 protein, human; D012333:RNA, Messenger