Carcinogen-induced inflammation and immunosuppression are enhanced in xeroderma pigmentosum group A model mice associated with hyperproduction of prostaglandin E2.
Xeroderma pigmentosum group A (XPA) gene-deficient mice easily developed skin cancers by the application of topical chemical carcinogens as well as by UV irradiation. As certain chemical carcinogens have been shown to be immunosuppressive, we examined the inflammatory and immunosuppressive effects of dimethylbenz(a)anthracene (DMBA) on XPA mice. Compared with wild-type mice, XPA mice showed greater ear swelling and reduction of epidermal Langerhans cells after DMBA application. Topical application of DMBA impaired the induction of contact hypersensitivity, initiated either locally or at distant sites. These DMBA-induced local and systemic immunosuppressions were more greatly enhanced in XPA mice than in wild-type mice. DMBA application induced pronounced production of PGE(2), IL-10, and TNF-alpha in the skin of XPA mice. Treatment with indomethacin, a potent inhibitor of PG biosynthesis, inhibited DMBA-induced inflammation and local immunosuppression. In XPA mice, increased serum IL-10 was detected after DMBA treatment. Excess production of PGE(2), TNF-alpha, and IL-10 after DMBA application may be involved in the enhanced local and systemic immunosuppression in DMBA-treated XPA mice. Susceptibility to DMBA-induced skin tumors in XPA mice may be due to easy impairment of the immune system by DMBA in addition to a defect in the repair of DMBA-DNA adduct. Enhanced immunosuppression by chemical carcinogens as well as the mutagenicity of these mutagens might be associated with the high incidence of internal malignancies seen in XP patients. Moreover, these results supported the hypothesis that persistent DNA damage is a trigger for the production of immunoregulatory cytokines.
Miyauchi-Hashimoto
H
H
Department of Dermatology, Kansai Medical University, Moriguchi, Japan. hashimoy@takaii.kmu.ac.jp
Kuwamoto
K
K
Urade
Y
Y
Tanaka
K
K
Horio
T
T
D015127:9,10-Dimethyl-1,2-benzanthracene / Q000037:antagonists & inhibitors / Q000633:toxicity*; D000276:Adjuvants, Immunologic / Q000037:antagonists & inhibitors / Q000633:toxicity; D000279:Administration, Cutaneous; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage; D001081:Apyrase / Q000096:biosynthesis; D002273:Carcinogens / Q000037:antagonists & inhibitors / Q000633:toxicity*; D004268:DNA-Binding Proteins / Q000235:genetics*; D003877:Dermatitis, Contact / Q000235:genetics / Q000276:immunology / Q000517:prevention & control; D004139:Dinitrofluorobenzene / Q000008:administration & dosage; D015232:Dinoprostone / Q000096:biosynthesis*; D004195:Disease Models, Animal; D004423:Ear / Q000473:pathology; D004487:Edema / Q000139:chemically induced / Q000235:genetics / Q000276:immunology / Q000517:prevention & control; D005260:Female; D007166:Immunosuppressive Agents / Q000037:antagonists & inhibitors / Q000633:toxicity*; D007213:Indomethacin / Q000008:administration & dosage; D007249:Inflammation / Q000139:chemically induced / Q000235:genetics / Q000276:immunology; D016753:Interleukin-10 / Q000096:biosynthesis / Q000097:blood / Q000378:metabolism; D007801:Langerhans Cells / Q000187:drug effects / Q000201:enzymology / Q000473:pathology; D051379:Mice; D008812:Mice, Hairless; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D018345:Mice, Knockout; D012867:Skin / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis; D015854:Up-Regulation / Q000235:genetics / Q000276:immunology; D014983:Xeroderma Pigmentosum / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D051760:Xeroderma Pigmentosum Group A Protein
D000276:Adjuvants, Immunologic; D000894:Anti-Inflammatory Agents, Non-Steroidal; D002273:Carcinogens; D004268:DNA-Binding Proteins; D007166:Immunosuppressive Agents; D014409:Tumor Necrosis Factor-alpha; D051760:Xeroderma Pigmentosum Group A Protein; C497315:Xpa protein, mouse; D016753:Interleukin-10; D015127:9,10-Dimethyl-1,2-benzanthracene; D004139:Dinitrofluorobenzene; D001081:Apyrase; D015232:Dinoprostone; D007213:Indomethacin