Proteomic analysis of the bacterial cell cycle.
A global approach was used to analyze protein synthesis and stability during the cell cycle of the bacterium Caulobacter crescentus. Approximately one-fourth (979) of the estimated C. crescentus gene products were detected by two-dimensional gel electrophoresis, 144 of which showed differential cell cycle expression patterns. Eighty-one of these proteins were identified by mass spectrometry and were assigned to a wide variety of functional groups. Pattern analysis revealed that coexpression groups were functionally clustered. A total of 48 proteins were rapidly degraded in the course of one cell cycle. More than half of these unstable proteins were also found to be synthesized in a cell cycle-dependent manner, establishing a strong correlation between rapid protein turnover and the periodicity of the bacterial cell cycle. This is, to our knowledge, the first evidence for a global role of proteolysis in bacterial cell cycle control.
Grünenfelder
B
B
Division of Molecular Microbiology, Biozentrum, University of Basel, Klingelbergstrasse 70, CH-4056 Basel, Switzerland.
Rummel
G
G
Vohradsky
J
J
Röder
D
D
Langen
H
H
Jenal
U
U
D016935:Caulobacter crescentus / Q000166:cytology* / Q000378:metabolism*; D002453:Cell Cycle; D015180:Electrophoresis, Gel, Two-Dimensional; D020543:Proteome
D020543:Proteome