Study on the secretion of cytokines from osteoblasts stimulated with prostaglandin E1.
In previous studies, prostaglandin (PG) E1 was found not only to enhance the effect of rhBMP, but also to increase orthotopic osteoconduction and enhance bone formation, even when used alone, as well as increasing the physical strength of implanted porous hydroxyapatite ceramics (HAP). In the present study, the secretion of cytokines from MC3T3-E1 cells was investigated, which is a cell line of osteoblasts derived from the mouse skull, stimulated in vitro by 10(-5) M and 10(-6) M PGE1, in order to elucidate the mechanism of action of PGE1. The cytokines examined included transforming growth factor (TGF) beta 1, interleukin (IL)-6, and IL-1 beta. The results of this study clarify the mechanism of enhancement of osteogenesis by PGE1. Elevated TGF beta 1 plays a very important role, and the findings are significant because they may provide the basis for the establishment of a rational clinical combined application of these materials.
Ono
I
I
Division of Plastic and Reconstructive Surgery, Department of Dermatology, Fukushima Medical University, School of Medicine, Hikarigaoka-1, Fukushima 960-1295, Japan. ichiro@fmu.ac.jp
Zhou
L J
LJ
Matsui
T
T
Kuboki
Y
Y
D016475:3T3 Cells; D000527:Alprostadil / Q000494:pharmacology*; D000704:Analysis of Variance; D000818:Animals; D016207:Cytokines / Q000096:biosynthesis*; D007375:Interleukin-1 / Q000096:biosynthesis; D015850:Interleukin-6 / Q000096:biosynthesis; D051379:Mice; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism*; D010012:Osteogenesis / Q000187:drug effects*; D016212:Transforming Growth Factor beta / Q000096:biosynthesis
D016207:Cytokines; D007375:Interleukin-1; D015850:Interleukin-6; D016212:Transforming Growth Factor beta; D000527:Alprostadil