Normal colonocytes in primary culture--an experimental model for molecular pharmacology and biology of large intestine.
A simple method of whole intestinal crypts isolation from rat's colonic tissue has been developed. Culture of viable epithelial cells (colonocytes) was obtained from intact crypts using method providing colonocytes for apoptosis. Satisfactory results have been obtained if the crypts were isolated using collagenase I. Under conditions applied, spontaneous release of the colonocytes took place. Liberated cells underwent almost immediate adhesion to microcarrier surface. The primary culture of normal colonocytes indicating metabolic activity for long time (> 10 days) has been obtained.
Dzierzewicz
Z
Z
Department of Molecular Biology, Biochemistry and Biopharmacy, Medical University of Silesia, 1 Narcyzów Str., 41-200 Sosnowiec.
Orchel
A
A
Swierczek-Zieba
G
G
Latocha
M
M
Cwalina
B
B
Wilczok
T
T
D000818:Animals; D017209:Apoptosis; D002478:Cells, Cultured; D003106:Colon / Q000166:cytology*; D004492:Edetic Acid / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
D004492:Edetic Acid