Effects of 3-methylcholanthrene and aspirin co-administration on ALDH3A1 in HepG2 cells.
The effects of two different protocols of 3-methylcholanthrene (3MC) and aspirin co-administration were studied in a well-established human hepatoma cell line (HepG2). During this work, we have performed toxicity tests for cell viability/cell proliferation as well as studies on the expression of ALDH3A1 after exposure of HepG2 cells to 3MC or/and aspirin. For the evaluation of toxic concentrations of 3MC and aspirin, the WST-1 test was used. WST-1 is a reliable cytotoxicity test which is based on the cleavage of the tetrazolium salt WST-1 to formazan by mitochondrial enzymes of living cells. A broad range of drug concentrations for either 3MC (0.25-50.0 microM) or aspirin (0.05-10.0 mM) were used for cell exposure, in several periods of time. The expression of ALDH3A1 in HepG2 cells showed typical time- and dose-response curves of induction after application of 3MC (1-5 days, 1.5-5.0 microM, respectively). When cells were firstly exposed to 3MC (2.5 and 5.0 microM) and then to aspirin (0.25 mM), the induced ALDH3A1 activity was further enhanced in a statistically significant way (P<0.05). On the contrary, when aspirin application was preceded 3MC exposuring a statistically significant decrease in ALDH3A1 inducibility was observed, as compared with the application of 3MC alone.
Sotiropoulou
M
M
Department of Pharmacology, University of Ioannina, Medical School, 451-10, Ioannina, Greece. me00189@cc.uoi.gr
Pappas
P
P
Marselos
M
M
D000444:Aldehyde Dehydrogenase / Q000378:metabolism*; D001241:Aspirin / Q000008:administration & dosage*; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy* / Q000201:enzymology* / Q000473:pathology; D002470:Cell Survival / Q000187:drug effects; D004347:Drug Interactions; D005982:Glutathione Transferase / Q000378:metabolism; D006801:Humans; D008113:Liver Neoplasms / Q000188:drug therapy* / Q000201:enzymology* / Q000473:pathology; D008748:Methylcholanthrene / Q000008:administration & dosage*; D014407:Tumor Cells, Cultured
C030863:corneal protein 54, bovine; D008748:Methylcholanthrene; D000444:Aldehyde Dehydrogenase; D005982:Glutathione Transferase; D001241:Aspirin