title: Altered expression of the lymphocyte activation markers CD30 and CD27 in patients with pouchitis.
abstract: BACKGROUND
  The mechanism underlying the development of ileal pouch inflammation in ulcerative colitis patients (pouchitis) after restorative proctocolectomy is unclear. Persistent systemic T cell activation or expansion of specific memory cell populations could predispose certain patients to develop local inflammation within the neo-rectum. Therefore, the aim was to study the expression of the lymphocyte activation markers CD27, CD30, CD25 and CD69 on the CD45RO+ memory cell subset of isolated peripheral blood mononuclear cells (PBMC), soluble CD30 levels and mucosal CD30 expression in patients with pouchitis and in controls.
  
  
  METHODS
  Flow cytometry was performed on PBMC isolated from patients with pouchitis (n = 9), without pouchitis (n = 10) and normal controls (n = 9). Serum CD30 was measured in patients with pouchitis (n = 25), without pouchitis (n = 26) and normal controls (n = 20) by ELISA. CD30 expression was quantified in pouchitis (n = 15) and normal pouch (n = 15) mucosa using a three-stage immunoperoxidase method.
  
  
  RESULTS
  Naive CD45RO-CD27+ PBMC were significantly decreased in pouchitis (25.6%) compared to normal controls (34.4%), (P = 0.03). CD30, CD25 and CD69 subsets did not differ between the groups. Serum CD30 was increased in pouchitis patients 58 (1-380) U/ml compared to non-pouchitis 16.5 (1-290) U/ml, P=0.007, and normal controls 11 (2-80) U/ml, P = 0.0005. In the mucosa, the numbers of CD30+ cells were increased in pouchitis compared to non-inflamed pouches (P = 0.02).
  
  
  CONCLUSIONS
  Increased sCD30 in pouchitis is associated with elevated mucosal expression. Of the activation markers studied, only the circulating naïve CD27+ population differed in pouchitis patients compared with controls. The observed decrease in this cell type may reflect antigen priming and subsequent loss of CD27 implying that antigen driven activation of specific T cell subsets may occur in pouchitis.
authors: Gastroenterology Dept, St. Mark's Hospital, Harrow, Middlesex, UK.
authors: P D
authors: PD
authors: Thomas
authors: A
authors: A
authors: Forbes
authors: R J
authors: RJ
authors: Nicholls
authors: P J
authors: PJ
authors: Ciclitira
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000032:analysis; D001707:Biopsy, Needle; D003093:Colitis, Ulcerative / Q000473:pathology / Q000601:surgery; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D017730:Ki-1 Antigen / Q000032:analysis*; D008297:Male; D008875:Middle Aged; D009000:Monocytes; D010784:Photomicrography; D019449:Pouchitis / Q000097:blood* / Q000209:etiology / Q000473:pathology*; D011336:Probability; D016737:Proctocolectomy, Restorative / Q000009:adverse effects* / Q000379:methods; D012016:Reference Values; D012680:Sensitivity and Specificity; D018709:Statistics, Nonparametric; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7 / Q000032:analysis*
chemical_list: D015415:Biomarkers; D017730:Ki-1 Antigen; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7