Temporal temperature gradient gel electrophoresis (TTGE) as a tool for identification of Lactobacillus casei, Lactobacillus paracasei, Lactobacillus zeae and Lactobacillus rhamnosus.
OBJECTIVE
To develop a tool for rapid and inexpensive identification of the Lactobacillus casei complex.


RESULTS
Lactobacillus casei, Lactobacillus paracasei, Lactobacillus zeae and Lactobacillus rhamnosus were identified by PCR-amplification of the segment between the U1 and U2 regions of 16S rDNA (position 8-357, Escherichia coli numbering) and temporal temperature gradient gel electrophoresis (TTGE). Seven tested Lact. paracasei strains were divided into three TTGE-subgroups.


CONCLUSIONS
TTGE successfully distinguished between the closely-related target species. TTGE is also a powerful method for revealing sequence heterogeneities in the 16S rRNA genes.


CONCLUSIONS
Due to rapid and easy performance, TTGE of PCR-amplified 16S rDNA fragments will be useful for the identification of extended numbers of isolates.
Vásquez
A
A
Laboratory of Food Hygiene, Division of Food Technology, Lund University, Sweden.
Ahrné
S
S
Pettersson
B
B
Molin
G
G
D015373:Bacterial Typing Techniques / Q000379:methods; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D014644:Genetic Variation; D007778:Lactobacillus / Q000145:classification* / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods; D012329:RNA, Bacterial / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D013696:Temperature
D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S