Pretreatment with glycine reduces the severity of warm intestinal ischemic-reperfusion injury in the rat.
Free jejunal flaps may experience adverse effects immediately after revascularization because of ischemic-reperfusion injury. In this study the authors evaluated the ability of glycine to protect the small intestine against the effects of a warm ischemic-reperfusion injury. Male Wistar rats (N = 30) were randomized to either a baseline group (no intervention), a control group (local arterial infusion with normal saline), or a glycine group (local arterial infusion with 20% glycine). Pretreatment with 20% glycine increased significantly (p < 0.05) mucosal protein and deoxyribonucleic acid content, reduced intestinal myeloperoxidase activity, and maintained mucosal glutaminase activity. These results indicate that some of the indicators of ischemic-reperfusion injury are improved by pretreatment with a 20% glycine solution.
Lee
M A
MA
University Department of Surgery, Royal Perth Hospital, Australia.
McCauley
R D
RD
Kong
S E
SE
Hall
J C
JC
D000818:Animals; D016009:Chi-Square Distribution; D005998:Glycine / Q000008:administration & dosage / Q000494:pharmacology*; D007261:Infusions, Intra-Arterial; D007511:Ischemia / Q000473:pathology; D007583:Jejunum / Q000098:blood supply / Q000187:drug effects; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000473:pathology / Q000517:prevention & control*; D018709:Statistics, Nonparametric; D013524:Surgical Flaps / Q000098:blood supply*
D005998:Glycine