title: In situ hybridization analysis of transforming growth factor-beta 1 RNA expression during mouse tooth development.
abstract: OBJECTIVE
  The aim of this study is to investigate the temporal and spatial expression and localization of transforming growth factor-beta 1 mRNA during mouse tooth development.
  
  
  METHODS
  The distribution pattern of TGF-beta 1 mRNA during tooth development was analyzed on 5 microns serial sections of paraformaldehyde-fixed and paraffin-embedded embryonic mouse heads or mandibles.
  
  
  RESULTS
  The results show that TGF-beta 1 mRNA is expressed during mouse tooth development in a temporally and spatially regulated fashion. Local expression of TGF-beta 1 mRNA in the dental epithelium at budstaged (E13) and capstaged (E15) teeth were observed. During bell stage (E16-18), TGF beta 1 mRNA was very abundant in the ameloblast layer and dental papilla cells. The expression of TGF-beta 1 increased in the layer of odontoblast and ameloblast with the differentiation of these cells.
  
  
  CONCLUSIONS
  These results suggest that TGF-beta 1 may have an important role in odontogenesis and that it acts as a paracrine and autoinducing factor.
authors: School of Stomatology, Hubei Medical University, Wuhan, 430079, P. R. China.
authors: Q
authors: Q
authors: Zhu
authors: M
authors: M
authors: Fan
authors: Z
authors: Z
authors: Bian
authors: Z
authors: Z
authors: Chen
authors: Q
authors: Q
authors: Zhang
authors: B
authors: B
authors: Peng
mesh_terms: D000565:Ameloblasts / Q000166:cytology / Q000378:metabolism; D000818:Animals; D019898:Autocrine Communication / Q000235:genetics; D003771:Dental Papilla / Q000166:cytology / Q000378:metabolism; D004848:Epithelium / Q000196:embryology; D018507:Gene Expression Regulation, Developmental; D005865:Gestational Age; D017403:In Situ Hybridization; D051379:Mice; D009804:Odontoblasts / Q000166:cytology / Q000378:metabolism; D009805:Odontogenesis / Q000235:genetics*; D019899:Paracrine Communication / Q000235:genetics; D015347:RNA Probes; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics*; D014083:Tooth Germ / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000235:genetics*; D053773:Transforming Growth Factor beta1
chemical_list: D015347:RNA Probes; D012333:RNA, Messenger; C508961:Tgfb1 protein, mouse; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1