Genomic map of Clostridium perfringens strain 13.
A physical and genetic map of Clostridium perfringens strain 13 was constructed. C. perfringens strain 13 was found to have a 3.1-Mb chromosome and a large 50-kb plasmid, indicating that strain 13 has a relatively small genome among C. perfringens strains. A total of 313 genetic markers were mapped on the chromosome of strain 13. Compared with the physical and genetic map of C. perfringens CPN50, strain 13 had a quite similar genome organization, but with a large deletion (approximately 400 kb) in a particular segment of the chromosome. Among several toxin genes, a beta2 toxin gene that is a novel virulence gene in C. perfringens was found to be located on the 50-kb plasmid.
Shimizu
T
T
Department of Microbiology, Institute of Basic Medical Sciences, University of Tsukuba, Ibaraki, Japan. tshimizu@md.tsukuba.ac.jp
Ohshima
S
S
Ohtani
K
K
Shimizu
T
T
Hayashi
H
H
D001427:Bacterial Toxins / Q000235:genetics; D015139:Blotting, Southern; D002872:Chromosome Deletion; D002876:Chromosomes, Bacterial / Q000235:genetics*; D003001:Cloning, Molecular; D003016:Clostridium perfringens / Q000235:genetics* / Q000472:pathogenicity; D015342:DNA Probes; D016521:Electrophoresis, Gel, Pulsed-Field; D005798:Genes, Bacterial / Q000235:genetics*; D016680:Genome, Bacterial; D020161:Physical Chromosome Mapping; D010957:Plasmids / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D014774:Virulence / Q000235:genetics
D001427:Bacterial Toxins; D015342:DNA Probes