[Adenovirus-mediated herpes simplex virus thymidine kinase gene transference and combined drug therapy leads to apoptosis of human epithelial ovarian cancer cells].
OBJECTIVE
To evaluate the treatment and apoptosis effect of adenovirus-mediated herpes simplex virus thymidine kinase (HSV1-tk) gene transference followed by administration of ganciclovir (GCV) and acyclovir (ACV) on ovarian epithelial cancer cells.


METHODS
Recombinant adenovirus was amplificated and purified by routine method. The expression of HSV1-tk gene was assayed by polymerase chain reaction (PCR). The efficiency of recombinant Advtk transference was evaluated. The cytotoxicity efficacy of TYK cells that carry HSV1-tk gene was evaluated followed by GCV, ACV administration after the transference of Advtk. The changes and apoptosis of TYK cells that carry HSV1-tk gene were observed by means of analysis of DNA fragmentation and electronic microscopy.


RESULTS
Adenovirus was amplificated and purified in large amount. PCR assay showed 404 bp special band. When the multiplicities of infection (MOI) was 100, the transduction rate was 98.9%. The inhibition rates of TYK cells that carry HSV1-tk gene increased with the increase of MOI when the same concentration of GCV, ACV were given. When the MOI was same, the inhibition rates were also increased with the increase concentration of GCV, ACV. Apoptosis of TYK cells that carry HSV1-tk gene after administration of GCV was observed by means of analysis of DNA fragmentation and electronic microscopy.


CONCLUSIONS
The HSV1-tk gene can effectively transferred into TYK cells by recombinant replicated-deficient adenovirus vector, GCV, ACV can effectively kill TYK cells that carry HSV1-tk gene in vitro. Apoptosis may be the mechanism of the killing effect.
Jiang
J
J
Department of Obstetrics and Gynecology, Affiliated Hospital of Shandong Medical University, Jinan 250012, China.
Kong
B
B
Jiang
S
S
D000212:Acyclovir / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D000998:Antiviral Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D005260:Female; D015774:Ganciclovir / Q000494:pharmacology*; D018259:Herpesvirus 1, Human / Q000201:enzymology* / Q000235:genetics; D006801:Humans; D010051:Ovarian Neoplasms / Q000473:pathology; D013937:Thymidine Kinase / Q000235:genetics*; D014162:Transfection; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000970:Antineoplastic Agents; D000998:Antiviral Agents; D013937:Thymidine Kinase; D015774:Ganciclovir; D000212:Acyclovir