The chicken Pdcd4 gene is regulated by v-Myb.
The retroviral oncogene v-myb encodes a transcription factor (v-Myb) which is responsible for the ability of avian myeloblastosis virus (AMV) to transform myelomonocytic cells. v-Myb is thought to disrupt the differentiation of myelomonocytic cells by affecting the expression of specific target genes. To identify such genes we have analysed the gene expression in a myelomonocytic chicken cell line that carries an estrogen inducible version of v-Myb by differential display. Here we describe the identification of the chicken homolog of the mouse Pdcd4 gene as a novel v-Myb target gene. Pdcd4 is also known as MA-3, TIS and H731 and has recently been shown to suppress the transformation of epidermal cells by tumor promoters. Our results provide the first evidence that v-Myb directly regulates the expression of a potential tumor suppressor gene.
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Institut für Biochemie, Westfälische-Wilhelms-Universität Münster, Wilhelm-Klemm-Str. 2, D-48149 Münster, Germany.
Burk
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Worpenberg
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Klempnauer
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KH
D001355:Alpharetrovirus / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics / Q000528:radiation effects; D009189:Avian Myeloblastosis Virus / Q000235:genetics; D001483:Base Sequence; D002467:Cell Nucleus / Q000378:metabolism; D002478:Cells, Cultured; D002645:Chickens / Q000235:genetics*; D003561:Cytarabine / Q000494:pharmacology; D005786:Gene Expression Regulation; D008969:Molecular Sequence Data; D022423:Myeloid Cells / Q000502:physiology / Q000528:radiation effects / Q000821:virology; D020626:Oncogene Proteins v-myb / Q000235:genetics / Q000378:metabolism*; D011506:Proteins / Q000235:genetics* / Q000378:metabolism*; D016601:RNA-Binding Proteins; D017386:Sequence Homology, Amino Acid; D013950:Thymus Gland / Q000378:metabolism; D014466:Ultraviolet Rays
D020626:Oncogene Proteins v-myb; D011506:Proteins; D016601:RNA-Binding Proteins; D003561:Cytarabine