Corynebacterium diphtheriae surface proteins as adhesins to human erythrocytes.
Corynebacterium diphtheriae strains express non-fimbrial surface proteins able to recognize and bind to specific host cells receptors. Protein extracts were obtained from bacterial cells by mechanical process and ammonium sulfate precipitation at 25 and 45% (w/v) saturation. SDS-PAGE analysis of the extracts detected two polypeptide bands of 67 and 72 kDa, named 67-72 p. The 67-72 p, rabbit anti-67-72 p IgG antibodies as well as human gastric mucin, N-acetylneuraminic acid and N-acetyl D-glucosamine molecules were able to inhibit bacterial hemagglutination. Hemagglutination assays using 67-72 p-coated latex beads and Western blot analysis of biotin-labeled 67-72 p and erythrocyte receptors demonstrated the binding of 67-72 p to human erythrocyte membranes. Immunolabeled colloidal gold-A protein transmission electron microscopy using anti-67-72 p revealed a diffuse distribution of non-fimbrial 67-72 p on the surface of C. diphtheriae strains of both sucrose-fermenting and non-fermenting biotypes. Non-fimbrial lectin-like surface 67-72 p may play a role as adhesins in bacterial attachment thereby facilitating the early steps in pathogenesis of both toxigenic and non-toxigenic C. diphtheriae.
Colombo
A V
AV
Disciplina de Microbiologia e Imunologia, Faculdade De Ciências Médicas, Universidade do Estado do Rio de Janeiro, Brazil.
Hirata
R
R
de Souza
C M
CM
Monteiro-Leal
L H
LH
Previato
J O
JO
Formiga
L C
LC
Andrade
A F
AF
Mattos-Guaraldi
A L
AL
D000117:Acetylglucosamine / Q000494:pharmacology; D018829:Adhesins, Bacterial / Q000737:chemistry* / Q000302:isolation & purification; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry* / Q000302:isolation & purification; D003353:Corynebacterium diphtheriae / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000382:microbiology*; D006384:Hemagglutination / Q000187:drug effects; D006801:Humans; D016253:Microscopy, Immunoelectron; D008970:Molecular Weight; D019158:N-Acetylneuraminic Acid / Q000494:pharmacology; D011485:Protein Binding / Q000187:drug effects
D018829:Adhesins, Bacterial; D001425:Bacterial Outer Membrane Proteins; D019158:N-Acetylneuraminic Acid; D000117:Acetylglucosamine