Use of antiserum-coated latex particles for serotyping Streptococcus pneumoniae.
The Quellung reaction provides a standard means for serotyping Streptococcus pneumoniae, but it requires microscopic examination with skillful technique. We have developed an improved agglutination method with anti-rabbit IgG-coated latex particles, which are sensitized with pooled antisera for serotyping/serogrouping S. pneumoniae. Our method is as specific and sensitive as the Quellung test, and much easier to perform.
Arai
S
S
Infectious Diseases Surveillance Center, National Institute of Infectious Diseases, Tokyo, Japan.
Konda
T
T
Wad
A
A
Matsunaga
Y
Y
Okabe
N
N
Watanabe
H
H
Inouye
S
S
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology*; D000942:Antigens, Bacterial / Q000032:analysis / Q000276:immunology; D006801:Humans; D007106:Immune Sera / Q000276:immunology*; D007074:Immunoglobulin G / Q000276:immunology; D007841:Latex Fixation Tests / Q000379:methods*; D008826:Microbial Sensitivity Tests; D008863:Microspheres; D010400:Penicillin G / Q000494:pharmacology; D011817:Rabbits; D012680:Sensitivity and Specificity; D012703:Serotyping / Q000379:methods*; D013205:Staphylococcal Protein A / Q000276:immunology; D013296:Streptococcus pneumoniae / Q000145:classification* / Q000187:drug effects / Q000276:immunology* / Q000302:isolation & purification
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D007106:Immune Sera; D007074:Immunoglobulin G; D013205:Staphylococcal Protein A; D010400:Penicillin G