title: Effects of high ELF magnetic fields on enzyme-catalyzed DNA and RNA synthesis in vitro and on a cell-free DNA mismatch repair.
abstract: Environmental electromagnetic fields have been implicated in human cancers. We examined whether high extremely low frequency (ELF) AC magnetic fields could affect DNA synthesis, transcription or repair, using in vitro model systems with defined sequences. The rate and fidelity of DNA polymerase catalyzed DNA synthesis, as well as of RNA polymerase catalyzed RNA synthesis, were not statistically significantly affected by 60 Hz 0.25-0.5 Tesla magnetic fields. The efficiency of mutS dependent mismatch repair with human cell extracts was also not affected by the magnetic field exposure. The results suggest that the core processes related to the transmission of genetic information are stable under high ELF magnetic fields.
authors: Center for Biomedical Research and Education, School of Medicine, Kanazawa University, Ishikawa, Japan.
authors: S
authors: S
authors: Harada
authors: S
authors: S
authors: Yamada
authors: O
authors: O
authors: Kuramata
authors: Y
authors: Y
authors: Gunji
authors: M
authors: M
authors: Kawasaki
authors: T
authors: T
authors: Miyakawa
authors: H
authors: H
authors: Yonekura
authors: S
authors: S
authors: Sakurai
authors: K
authors: K
authors: Bessho
authors: R
authors: R
authors: Hosono
authors: H
authors: H
authors: Yamamoto
mesh_terms: D000818:Animals; D020137:Base Pair Mismatch; D002474:Cell-Free System; D004247:DNA / Q000096:biosynthesis; D004260:DNA Repair / Q000528:radiation effects; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism* / Q000528:radiation effects; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism* / Q000528:radiation effects; D004574:Electromagnetic Fields; D004926:Escherichia coli / Q000235:genetics / Q000528:radiation effects; D006367:HeLa Cells; D006801:Humans; D007700:Kinetics; D012313:RNA / Q000096:biosynthesis
chemical_list: D012313:RNA; D004247:DNA; D012321:DNA-Directed RNA Polymerases; D004259:DNA-Directed DNA Polymerase