Calprotectin expression by gingival epithelial cells.
Calprotectin, a heterodimer of MRP8 and MRP14 with antimicrobial properties, is found in the cytosol of neutrophils, monocytes, and human gingival keratinocytes. During inflammation of the oral mucosa, the expression of immunoreactive calprotectin appears upregulated. Given the possible cell sources, we sought to learn if epithelial cells upregulate calprotectin in response to proinflammmatory agents. First, human gingival keratinocytes were maintained in primary culture until senescence. At each passage, cells were harvested and analyzed for quantitative expression of MRP8 and MRP14 subunit mRNA by RNase protection assays and calprotectin complex by enzyme-linked immunosorbent assay. Calprotectin expression was constitutive in the primary gingival keratinocytes, but calprotectin-specific mRNA and protein tended to increase as the cells neared senescence. To test whether calprotectin expression was inducible, immortalized gingival keratinocyte cultures were treated for 2 to 4 h with lipopolysaccharide (LPS) or interleukin-1 beta (IL-1 beta). As a positive control for inducible expression, immortalized keratinocytes were incubated with phorbol myristate acetate (PMA) (50 ng/ml) for 24 h. Incubation with PMA stimulated increased expression of MRP8 and MRP14 mRNA within 2 h, peaking within 5 h. MRP8- and MRP14-specific mRNA expression by immortalized keratinocytes appeared to be unaffected by LPS or IL-1 beta. In contrast, LPS, IL-1 beta, and PMA each upregulated IL-8. These data show that calprotectin mRNA is expressed constitutively in cultured keratinocytes, while expression by immortalized cells appears to be independent of the exogenous proinflammatory agents LPS and IL-1 beta.
Ross
K F
KF
Department of Preventive Science, School of Dentistry, University of Minnesota, Minneapolis, Minnesota 55455, USA.
Herzberg
M C
MC
D000943:Antigens, Differentiation / Q000096:biosynthesis* / Q000235:genetics; D002135:Calcium-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics; D040501:Calgranulin A; D040502:Calgranulin B; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000378:metabolism; D005881:Gingiva / Q000166:cytology / Q000378:metabolism*; D000949:Histocompatibility Antigens Class II / Q000096:biosynthesis; D006801:Humans; D007150:Immunohistochemistry; D016209:Interleukin-8 / Q000235:genetics; D015603:Keratinocytes / Q000378:metabolism; D039841:Leukocyte L1 Antigen Complex; D008070:Lipopolysaccharides / Q000494:pharmacology; D008562:Membrane Glycoproteins / Q000096:biosynthesis; D019006:Neural Cell Adhesion Molecules / Q000096:biosynthesis; D012333:RNA, Messenger / Q000032:analysis; D009418:S100 Proteins / Q000096:biosynthesis* / Q000235:genetics
D000943:Antigens, Differentiation; D002135:Calcium-Binding Proteins; D040501:Calgranulin A; D040502:Calgranulin B; D000949:Histocompatibility Antigens Class II; D016209:Interleukin-8; D039841:Leukocyte L1 Antigen Complex; D008070:Lipopolysaccharides; D008562:Membrane Glycoproteins; D019006:Neural Cell Adhesion Molecules; D012333:RNA, Messenger; D009418:S100 Proteins