title: Activated in prostate cancer: a PDZ domain-containing protein highly expressed in human primary prostate tumors.
abstract: Critical events in prostate tumorigenesis and metastasis likely include the abnormal activation and expression of specific genes. Using RNA expression profiling techniques, we have identified a transcript originating from the activated in prostate cancer (AIPC) gene, the expression of which is preferentially up-regulated in several cultured prostate tumor cell lines and human primary prostate tumors. Sequence analysis revealed that the AIPC protein encodes six PDZ domains, which are protein-protein binding domains likely involved in protein clustering and scaffolding. Immunohistochemical analysis of a tissue microarray comprising 158 tumor, 18 high-grade prostatic intraepithelial neoplasia, and 91 normal prostate specimens with an anti-AIPC antibody demonstrated abundant AIPC protein expression in 75% of tumors, 83% of prostatic intraepithelial neoplasia lesions, and 3% of normal tissues (P < 0.0001). These data suggest that the accumulation of AIPC protein may be closely associated with the initiation or early promotion of prostate tumorigenesis.
authors: The Department of Surgery, The University of Michigan, Ann Arbor 48109-0946, USA.
authors: H
authors: H
authors: Chaib
authors: M A
authors: MA
authors: Rubin
authors: N R
authors: NR
authors: Mucci
authors: L
authors: L
authors: Li
authors: Taylor JMG
authors: M L
authors: ML
authors: Day
authors: J S
authors: JS
authors: Rhim
authors: J A
authors: JA
authors: Macoska
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D001483:Base Sequence; D001665:Binding Sites; D015815:Cell Adhesion Molecules; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics* / Q000502:physiology; D020411:Oligonucleotide Array Sequence Analysis; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D014407:Tumor Cells, Cultured; D015854:Up-Regulation
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D015815:Cell Adhesion Molecules; D009363:Neoplasm Proteins; C423943:PDZD2 protein, human; D012333:RNA, Messenger