Determination of canine beta2-microgloblin in plasma and urine by enzyme-linked immunosorbent assay.
An enzyme-linked immunosorbent assay was developed for the determination of canine beta2-microglobulin (beta2-m) in plasma and urine. The detectable sensitivity for pure canine beta2-m was 0.05 microg/l and the analytical range was 0.1 to 50 microg/l. The mean analytical recovery when pure canine beta2-m was added to normal plasma was 101.9%. The mean analytical recovery in the urine was 102.1%. The intra-day variation coefficient was 3.1% in plasma, 4.3% in serum and 1.9% in urine. No difference was found between the concentration of beta2-m in plasma and serum (n=17). The concentration of beta2-m in the plasma of normal dogs was 1.82 +/- 0.57 mg/l (n=31). The mean excretion in 24 hr urine collected from normal dogs was 17.6 +/- 9.2 microg/l, 0.22 +/- 0.12 microg/kg of body weight or 14.2 +/- 9.4 microg/g of urine creatinine. The beta2-m creatinine index of random urine samples was 23.5 +/- 16.6 microg/g (n=26). There was a close correlation between the beta2-m creatinine index of 24 hr urine samples and that of random urine samples (r=0.872).
Nakajima
Y
Y
Laboratory, of Veterinary Internal Medicine, School of Veterinary Medicine and Animal Sciences, Kitasato University, Towada, Aomori, Japan.
Hoshi
F
F
Higuchi
S
S
Kawamura
S
S
D000818:Animals; D003404:Creatinine / Q000652:urine; D004285:Dogs / Q000097:blood* / Q000652:urine*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D012680:Sensitivity and Specificity; D001613:beta 2-Microglobulin / Q000097:blood* / Q000652:urine*
D001613:beta 2-Microglobulin; D003404:Creatinine