Markers for oxidative stress associated with soft rots in French beans (Phaseolus vulgaris) infected by Botrytis cinerea.
The role of active oxygen species has been studied in spreading soft-rot lesions caused by the necrotrophic fungal pathogen Botrytis cinerea Pers.:Fr. in leaves of four genotypes of French bean (Phaseolus vulgaris L.). Large increases were observed for the aldehydic end-products of oxidative damage, malondialdehyde and 4-hydroxy-2-nonenal, as a result of infection in each of the genotypes studied. Similar increases were found in a stable free radical and g=4.27 Fe(III) signals, but not Mn(II) signals, in electron paramagnetic resonance spectra. These changes were accompanied by large decreases in ascorbic acid levels, with changes in the antioxidant glutathione being genotype dependent.
Muckenschnabel
I
I
Scottish Crop Research Institute, Invergowrie, Dundee, UK.
Williamson
B
B
Goodman
B A
BA
Lyon
G D
GD
Stewart
D
D
Deighton
N
N
D000447:Aldehydes / Q000378:metabolism; D001205:Ascorbic Acid / Q000378:metabolism; D015415:Biomarkers / Q000032:analysis; D020171:Botrytis; D004578:Electron Spin Resonance Spectroscopy; D007887:Fabaceae / Q000378:metabolism*; D005609:Free Radicals / Q000378:metabolism; D005838:Genotype; D005978:Glutathione / Q000378:metabolism; D066298:In Vitro Techniques; D015227:Lipid Peroxidation / Q000502:physiology; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000502:physiology*; D010935:Plant Diseases; D018515:Plant Leaves / Q000378:metabolism; D010946:Plants, Medicinal
D000447:Aldehydes; D015415:Biomarkers; D005609:Free Radicals; D008315:Malondialdehyde; D005978:Glutathione; C027576:4-hydroxy-2-nonenal; D001205:Ascorbic Acid