NID67, a small putative membrane protein, is preferentially induced by NGF in PC12 pheochromocytoma cells.
In an effort to identify genes involved in neuronal differentiation, we have used representational difference analysis (RDA) to clone cDNAs that are preferentially induced by nerve growth factor (NGF) vs. epidermal growth factor (EGF) in PC12 pheochromocytoma cells. We now report the cloning of a previously unknown primary response gene, NID67. In addition to a robust induction by NGF and FGF, both of which cause PC12 cells to differentiate, NID67 is strongly induced by forskolin, A23187 and ATP. EGF, TPA and KCl induce NID67 only weakly. NID67 mRNA is most abundant in heart, ovary and adrenal. Modest levels are present in most brain regions, testis, thyroid, thymus, pituitary, kidney and intestine; little NID67 is present in skeletal muscle and cerebellum. The NID67 cDNA contains a 180 bp open reading frame (ORF) that encodes a 60 amino acid protein. The central 29 amino acids are very hydrophobic and very likely comprise a transmembrane domain. Mouse and human NID67 cDNAs contain an ORF similar to NID67; the rat and human protein sequences are 85% identical whereas the rat and mouse sequences are 92% identical. In vitro transcription and translation reactions confirmed that the ORF we identified produces a 6000 Da protein product. Several small membrane proteins are similar to NID67; they contain a transmembrane domain and little more. All of these proteins participate in forming or regulating ion channels. NID67 may play a similar role in cellular physiology.
Vician
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L
Department of Biological Chemistry, UCLA Center for the Health Sciences, Los Angeles, California, USA.
Silver
A L
AL
Farias-Eisner
R
R
Herschman
H R
HR
D000310:Adrenal Gland Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000502:physiology; D002454:Cell Differentiation / Q000187:drug effects; D002895:Chromosomes, Human, Pair 5 / Q000235:genetics; D005576:Colforsin / Q000494:pharmacology; D016895:Culture Media, Serum-Free; D018076:DNA, Complementary / Q000235:genetics; D004273:DNA, Neoplasm / Q000235:genetics; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005260:Female; D005346:Fibroblast Growth Factors / Q000494:pharmacology; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006801:Humans; D007473:Ion Channels / Q000502:physiology; D007476:Ionophores / Q000494:pharmacology; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D009206:Myocardium / Q000378:metabolism; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D020932:Nerve Growth Factor / Q000494:pharmacology*; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics; D016366:Open Reading Frames; D009928:Organ Specificity; D010053:Ovary / Q000378:metabolism; D016716:PC12 Cells / Q000187:drug effects* / Q000378:metabolism; D010673:Pheochromocytoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D016133:Polymerase Chain Reaction; D011189:Potassium Chloride / Q000494:pharmacology; D017434:Protein Structure, Tertiary; D051381:Rats; D015290:Second Messenger Systems; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D016895:Culture Media, Serum-Free; D018076:DNA, Complementary; D004273:DNA, Neoplasm; D007473:Ion Channels; D007476:Ionophores; D008565:Membrane Proteins; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; C430225:SMIM3 protein, human; C498967:Smim3 protein, mouse; C498968:Smim3 protein, rat; D005576:Colforsin; D000001:Calcimycin; D005346:Fibroblast Growth Factors; D004815:Epidermal Growth Factor; D011189:Potassium Chloride; D020932:Nerve Growth Factor; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium