Immunohistochemical analysis of cellular infiltrate and gamma interferon, interleukin-12, and inducible nitric oxide synthase expression in leprosy type 1 (reversal) reactions before and during prednisolone treatment.
The effects of prednisolone treatment on the cellularity and cytokine (gamma interferon, interleukin-12, and inducible nitric oxide synthase) profiles of leprosy skin type 1 (reversal) reactions were studied using immunohistochemistry. Skin biopsies were taken from 15 patients with leprosy type 1 (reversal) reactions at days 0, 7, 28, and 180 after the start of steroid treatment. Prednisolone treatment had little effect at day 7, but by day 28 significant decreases were found in cytokine levels. Some patients maintained cytokine production at days 28 and 180. These results illustrate the strong Th1 profile of type 1 reactional lesions, the slow response to steroid therapy, and continuing activity at 180 days.
Little
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D
Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, United Kingdom.
Khanolkar-Young
S
S
Coulthart
A
A
Suneetha
S
S
Lockwood
D N
DN
D006801:Humans; D007150:Immunohistochemistry; D007371:Interferon-gamma / Q000032:analysis*; D018664:Interleukin-12 / Q000032:analysis*; D007918:Leprosy / Q000188:drug therapy / Q000276:immunology* / Q000473:pathology; D019001:Nitric Oxide Synthase / Q000032:analysis*; D052247:Nitric Oxide Synthase Type II; D011239:Prednisolone / Q000627:therapeutic use*
D018664:Interleukin-12; D007371:Interferon-gamma; D011239:Prednisolone; C496319:NOS2 protein, human; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II