title: Cajal-Retzius cells in early postnatal mouse cortex selectively express functional metabotropic glutamate receptors.
abstract: Glutamate receptors have been linked to the regulation of several developmental events in the CNS. By using cortical slices of early postnatal mice, we show that in layer I cells, glutamate produces intracellular calcium ([Ca(2+)](i)) elevations mediated by ionotropic and metabotropic glutamate receptors (mGluRs). The contribution of mGluRs to these responses was demonstrated by application of tACPD, an agonist to groups I and II mGluRs, which evoked [Ca(2+)](i) increases that could be reversibly blocked by MCPG, an antagonist to groups I and II mGluRs. In the absence of extracellular Ca(2+), repetitive applications of tACPD or quisqualate, an agonist to group I mGluRs, elicited decreasing [Ca(2+)](i) responses that were restored by refilling a thapsigargin-sensitive Ca(2+) store. The use of specific group I mGluR agonists CHPG and DHPG indicated that the functional mGluR in layer I was of the mGluR1 subtype. Subtype specific antibodies confirmed the presence of mGlur1 alpha, but not mGluR5, in Cajal-Retzius (Reelin-immunoreactive) neurons.
authors: Instituto de Neurociencias, CSIC, Universidad Miguel Hernández, Campus de San Juan, Apartado 18, 03550 San Juan de Alicante, Spain.
authors: J R
authors: JR
authors: Martínez-Galán
authors: G
authors: G
authors: López-Bendito
authors: R
authors: R
authors: Luján
authors: R
authors: R
authors: Shigemoto
authors: A
authors: A
authors: Fairén
authors: M
authors: M
authors: Valdeolmillos
mesh_terms: D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D002540:Cerebral Cortex / Q000166:cytology / Q000378:metabolism*; D007425:Intracellular Membranes / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D064529:Receptor, Metabotropic Glutamate 5; D018094:Receptors, Metabotropic Glutamate / Q000819:agonists / Q000378:metabolism*; D000091042:Reelin Protein
chemical_list: C578936:Grm5 protein, mouse; D064529:Receptor, Metabotropic Glutamate 5; D018094:Receptors, Metabotropic Glutamate; D000091042:Reelin Protein; C104077:metabotropic glutamate receptor type 1; C000717128:Reln protein, mouse; D002118:Calcium