High-performance liquid chromatographic analysis for the determination of buspirone analogue--mesmar in rat plasma.
A simple and selective analytical method for the determination of buspirone analogue--mesmar in rat plasma is described. The procedure involves liquid-liquid extraction followed by reversed-phase high-performance chromatographic analysis with UV detection at 245 nm. No endogenous compounds were found to interfere. The absolute extraction recovery from plasma samples was 76%. The linearity was assessed in the range 0.12-2.44 nmol/ml. Stability of mesmar during processing (autosampler) and in plasma was checked. This method proved suitable for pharmacokinetic studies following single oral dose in rats.
Kobylińska
K
K
Pharmacology Department, Pharmaceutical Research Institute, 8 Rydygiera Street, 01-793 Warsaw, Poland.
Cendrowska
I
I
Kobylińska
M
M
Barlińska
M
M
D000818:Animals; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D004355:Drug Stability; D008297:Male; D010879:Piperazines / Q000097:blood* / Q000737:chemistry; D011619:Psychotropic Drugs / Q000097:blood*; D051381:Rats; D017208:Rats, Wistar
D010879:Piperazines; D011619:Psychotropic Drugs; C093619:mesmar