Expression of interleukin-8 in human metastatic endometrial carcinoma cells and its regulation by inflammatory cytokines.
In the present study, we analyzed the expression of a multifunctional cytokine, interleukin-8 (IL-8), in metastatic endometrial carcinoma cells. Our data demonstrate that human serous papillary endometrial adenocarcinoma (SPEC) and human endometrial adenocarcinoma (HEC) cells expressed steady-state IL-8-specific mRNA transcript and secreted IL-8 protein. The levels of IL-8 mRNA in SPEC-2 cells established from stage IV serous papillary adenocarcinoma were three-fold higher as compared to endometrial adenocarcinoma cells, HEC-1 A, established from stage IA endometrial cancer. Further, we observed higher levels of IL-8 mRNA and protein expression in the metastatic variants of SPEC-2 and HEC-1A cells as compared to the parent cell lines, demonstrating that IL-8 expression was associated with metastatic potential. Further, the treatment of endometrial carcinoma cells with inflammatory cytokines, IL-1beta and tumor necrosis factor-alpha (TNF-alpha), demonstrated that IL-1beta and TNF-alpha induced IL-8 expression in endometrial cancer cells. IL-1beta was a more potent inducer of IL-8 expression than TNF-alpha in our studies. These data demonstrate that constitutive and induced IL-8 expression in endometrial carcinoma cells might be an important regulatory mechanism of tumor growth and metastasis.
Berry
K K
KK
Department of Cancer Biology, University of Texas, M. D. Anderson Cancer Center, Houston, Texas, USA.
Varney
M L
ML
Dave
B J
BJ
Bucana
C D
CD
Fidler
I J
IJ
Singh
R K
RK
D002291:Carcinoma, Papillary / Q000473:pathology*; D016207:Cytokines / Q000494:pharmacology*; D016889:Endometrial Neoplasms / Q000473:pathology*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D016209:Interleukin-8 / Q000096:biosynthesis*; D009362:Neoplasm Metastasis / Q000503:physiopathology; D012333:RNA, Messenger / Q000032:analysis; D014407:Tumor Cells, Cultured
D016207:Cytokines; D016209:Interleukin-8; D012333:RNA, Messenger