Comparison of the fluorescent polarization (TDx) and the enzymatic competitive (EMIT 2000) immune assays for the measurement of cyclosporin A blood concentration.
Evaluation of Cyclosporin A (CyA) blood concentration is imperative in solid organ transplantation in order to achieve maximal immunosuppression with the least side effects. We compared the results of whole blood concentrations of CyA in 50 blood samples simultaneously evaluated by the fluorescent polarization immune assay (TDx) and the enzymatic competitive immune assay (EMIT 2000). There was a strong correlation between both kits for any range of CyA blood concentration (R=0.99, p<0.001). The within-run and between-days coefficient of variation were less than 4% for both assays. The cost for each CyA measurement was 50% lower for the EMIT assay when compared to the TDx assay. We concluded that the EMIT is as accurate as the TDx in measuring CyA blood concentration and has the advantage of a lower cost, as well as the possibility of widespread access to the EMIT methodology in contrast to the TDx equipment, allowing the laboratory to perform several routines within a working day.
David-Neto
E
E
Hospital Sírio Libanes.
Ballarati
C A
CA
Freitas
O J
OJ
Lemos
F C
FC
Nahas
W C
WC
Arap
S
S
Kalil
J
J
D003365:Costs and Cost Analysis; D016572:Cyclosporine / Q000097:blood*; D016231:Fluorescence Polarization Immunoassay / Q000379:methods*; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods*; D007166:Immunosuppressive Agents / Q000097:blood*; D016030:Kidney Transplantation
D007166:Immunosuppressive Agents; D016572:Cyclosporine