Tissue-engineered product: allogeneic cultured dermal substitute composed of spongy collagen with fibroblasts.
Recently, various types of allogeneic skin substitutes including cultured epidermal substitute (CES), cultured dermal substitute (CDS), and cultured skin substitute (CSS), which are composed of keratinocytes and/or fibroblasts as the cellular component(s), have been used as biological wound dressings. In our study, the allogeneic CDS was prepared by plating fibroblasts on a spongy collagen. The clinical evaluation was conducted using fresh or cryopreserved allogeneic CDS. In 145 of our clinical cases, 95% (138/145) of various wounds were evaluated as achieving good or excellent results, including 96% (22/23) of deep dermal burns (DDB) and dermal burns (DB), 100% (53/53) of partial-thickness donor wounds, 91% (21/23) of traumatic skin defects, 100% (5/5) of pressure ulcers, 82% (9/11) of chronic skin ulcers, 100% (6/6) of coverage for debrided DB, and 92% (22/24) of coverage for autologous meshed graft. The results obtained in our study suggest that the allogeneic CDS is able to provide an effective therapy for patients with partial and/or full-thickness skin defects.
Kuroyanagi
Y
Y
R & D Center for Artificial Skin, School of Allied Health Sciences, Kitasato University, Sagamihara, Japan.
Yamada
N
N
Yamashita
R
R
Uchinuma
E
E
D000368:Aged; D001698:Biomedical Engineering; D002056:Burns / Q000628:therapy; D002478:Cells, Cultured; D003094:Collagen; D020405:Dermis / Q000166:cytology*; D005347:Fibroblasts / Q000166:cytology*; D006801:Humans; D008297:Male; D012883:Skin Ulcer / Q000628:therapy; D017768:Skin, Artificial; D014945:Wound Healing
D003094:Collagen