CD91 is a common receptor for heat shock proteins gp96, hsp90, hsp70, and calreticulin.
Complexes of the heat shock protein gp96 and antigenic peptides are taken up by antigen-presenting cells and presented by MHC class I molecules. In order to explain the unusual efficiency of this process, the uptake of gp96 had been postulated to occur through a receptor, identified recently as CD91. We show here that complexes of peptides with heat shock proteins hsp90, calreticulin, and hsp70 are also taken up by macrophages and dendritic cells and re-presented by MHC class I molecules. All heat shock proteins utilize the CD91 receptor, even though some of the proteins have no homology with each other. Postuptake processing of gp96-chaperoned peptides requires proteasomes and the transporters associated with antigen processing, utilizing the classical endogenous antigen presentation pathway.
Basu
S
S
Center for Immunotherapy of Cancer and Infectious Diseases, University of Connecticut School of Medicine, MC1601, Farmington, CT 06030, USA.
Binder
R J
RJ
Ramalingam
T
T
Srivastava
P K
PK
D000818:Animals; D017951:Antigen Presentation; D000951:Antigens, Neoplasm / Q000378:metabolism*; D001853:Bone Marrow; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D037282:Calreticulin; D002478:Cells, Cultured; D003546:Cysteine Endopeptidases / Q000378:metabolism; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D017353:Gene Deletion; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D018841:HSP90 Heat-Shock Proteins / Q000378:metabolism*; D015395:Histocompatibility Antigens Class I / Q000276:immunology; D006801:Humans; D026503:Low Density Lipoprotein Receptor-Related Protein-1; D016177:Macrophage-1 Antigen / Q000032:analysis; D017737:Macrophages, Peritoneal / Q000276:immunology / Q000378:metabolism; D051379:Mice; D009097:Multienzyme Complexes / Q000378:metabolism; D029543:Nucleocytoplasmic Transport Proteins; D010455:Peptides / Q000276:immunology / Q000378:metabolism; D046988:Proteasome Endopeptidase Complex; D011485:Protein Binding; D011506:Proteins / Q000235:genetics / Q000378:metabolism; D016601:RNA-Binding Proteins; D011971:Receptors, Immunologic / Q000378:metabolism*; D012261:Ribonucleoproteins / Q000378:metabolism*; D013379:Substrate Specificity; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014407:Tumor Cells, Cultured; D000511:alpha-Macroglobulins / Q000378:metabolism
D000951:Antigens, Neoplasm; D002135:Calcium-Binding Proteins; D037282:Calreticulin; D018840:HSP70 Heat-Shock Proteins; D018841:HSP90 Heat-Shock Proteins; D015395:Histocompatibility Antigens Class I; D026503:Low Density Lipoprotein Receptor-Related Protein-1; D016177:Macrophage-1 Antigen; D009097:Multienzyme Complexes; C122111:NXF1 protein, human; D029543:Nucleocytoplasmic Transport Proteins; D010455:Peptides; D011506:Proteins; D016601:RNA-Binding Proteins; D011971:Receptors, Immunologic; D012261:Ribonucleoproteins; D000511:alpha-Macroglobulins; C052646:sarcoma glycoprotein gp96 rejection antigens; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex