title: Recovery from arterial growth delay reduces penetrance of cardiovascular defects in mice deleted for the DiGeorge syndrome region.
abstract: Chromosome 22q11.2 heterozygous deletions cause the most common deletion syndrome, including the DiGeorge syndrome phenotype. Using a mouse model of this deletion (named Df1) we show that the aortic arch patterning defects that occur in heterozygously deleted mice (Df1/+) are associated with a differentiation impairment of vascular smooth muscle in the 4th pharyngeal arch arteries (PAAs) during early embryogenesis. Using molecular markers for neural crest, endothelial cells and vascular smooth muscle, we show that cardiac neural crest migration into the 4th arch and initial formation of the 4th PAAs are apparently normal in Df1/+ embryos, but affected vessels are growth-impaired and do not acquire vascular smooth muscle. As in humans, not all deleted mice present with cardiovascular defects at birth. However, we found, unexpectedly, that all Df1/+ embryos have abnormally small 4th PAAs during early embryogenesis. Many embryos later overcome this early defect, coincident with the appearance of vascular smooth muscle differentiation, and develop normally. Embryos born with aortic arch patterning defects probably represent a more severely affected group that fails to attain sufficient 4th PAA growth for normal remodelling of the PAA system. Our data indicate that Df1/+ embryos are able to overcome a localized arterial growth impairment and thereby reduce the penetrance of birth defects.
authors: Department of Pediatrics (Cardiology), Baylor College of Medicine, 1 Baylor Plaza, Houston, TX 77030, USA. elindsay@bcm.tmc.edu
authors: E A
authors: EA
authors: Lindsay
authors: A
authors: A
authors: Baldini
mesh_terms: D000818:Animals; D001013:Aorta, Thoracic / Q000002:abnormalities / Q000473:pathology; D001934:Branchial Region / Q000002:abnormalities* / Q000378:metabolism / Q000473:pathology; D004062:DiGeorge Syndrome / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D017353:Gene Deletion; D015870:Gene Expression; D005819:Genetic Markers; D006330:Heart Defects, Congenital / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D007124:Immunoenzyme Techniques; D017403:In Situ Hybridization; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009131:Muscle, Smooth, Vascular / Q000002:abnormalities* / Q000378:metabolism / Q000473:pathology; D009432:Neural Crest / Q000378:metabolism; D010641:Phenotype; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D005819:Genetic Markers; D001616:beta-Galactosidase