title: CD62L is required on effector cells for local interactions in the CNS to cause myelin damage in experimental allergic encephalomyelitis.
abstract: Adhesion molecules are believed to facilitate infiltration of leukocytes into the CNS of mice with experimental allergic encephalomyelitis (EAE). The role of the adhesion molecule CD62L (L-selectin) in the immunopathology of EAE is not known. To study this, we crossed CD62L-deficient mice with myelin basic protein-specific TCR (MBP-TCR) transgenic mice. CD62L-deficient MBP-TCR transgenic mice failed to develop antigen-induced EAE, and, despite the presence of leukocyte infiltration, damage to myelin in the CNS was not seen. EAE could, however, be induced in CD62L-deficient mice upon adoptive transfer of wild-type macrophages. Our results suggest that CD62L is not required for activation of autoimmune CD4 T cells but is important for the final destructive function of effector cells in the CNS and support a novel mechanism whereby CD62L expressed on effector cells is important in mediating myelin damage.
authors: Department of Immunology, Genentech Incorporated, South San Francisco, CA 94080, USA.
authors: I S
authors: IS
authors: Grewal
authors: H G
authors: HG
authors: Foellmer
authors: K D
authors: KD
authors: Grewal
authors: H
authors: H
authors: Wang
authors: W P
authors: WP
authors: Lee
authors: D
authors: D
authors: Tumas
authors: C A
authors: CA
authors: Janeway
authors: R A
authors: RA
authors: Flavell
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology / Q000378:metabolism / Q000637:transplantation; D015551:Autoimmunity / Q000235:genetics / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism / Q000637:transplantation; D002448:Cell Adhesion; D002490:Central Nervous System / Q000378:metabolism* / Q000473:pathology; D002634:Chemotaxis, Leukocyte; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000378:metabolism* / Q000473:pathology*; D017353:Gene Deletion; D007150:Immunohistochemistry; D019041:L-Selectin / Q000235:genetics / Q000378:metabolism*; D008213:Lymphocyte Activation; D008264:Macrophages / Q000276:immunology / Q000378:metabolism / Q000637:transplantation; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D004676:Myelin Basic Protein / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D009186:Myelin Sheath / Q000378:metabolism / Q000473:pathology*; D011948:Receptors, Antigen, T-Cell / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism
chemical_list: D004676:Myelin Basic Protein; D011948:Receptors, Antigen, T-Cell; D011993:Recombinant Fusion Proteins; D019041:L-Selectin