title: Pregnancy after intentional cryopreservation of percutaneous sperm aspiration specimens and intracytoplasmic sperm injection. A report of two cases.
abstract: BACKGROUND
  Microsurgical epididymal sperm aspiration (MESA) and percutaneous epididymal sperm aspiration (PESA) are two methods of obtaining spermatozoa from patients with irreparable obstructive azoospermia. Intentionally using frozen-thawed spermatozoa obtained from MESA had been reported to be successful. With minimal invasiveness, intentionally cryopreserved PESA specimens and intracytoplasmic sperm injection (ICSI) were carried out in two cases.
  
  
  METHODS
  Two cases of irreparable obstructive azoospermia received PESA, and the spermatozoa were cryopreserved intentionally. Successful ICSI was performed later, utilizing frozen-thawed spermatozoa.
  
  
  CONCLUSIONS
  PESA and ICSI are promising methods for these patients. The major advantages are minimal invasiveness and flexibility for further treatment.
authors: Department of Obstetrics and Gynecology, Shin-Kong Wu Ho-Su Memorial Hospital, No. 95, Wen Chang Road, Shih Lin District, Taipei 111, Taiwan, R.O.C.
authors: J L
authors: JL
authors: Hwang
authors: Y H
authors: YH
authors: Lin
authors: S C
authors: SC
authors: Huang
authors: M L
authors: ML
authors: Hsieh
authors: B C
authors: BC
authors: Hsieh
authors: Y L
authors: YL
authors: Tsai
mesh_terms: D000328:Adult; D015925:Cryopreservation; D005260:Female; D006801:Humans; D001239:Inhalation; D008297:Male; D008866:Microsurgery; D009845:Oligospermia / Q000209:etiology; D010062:Ovulation Induction / Q000379:methods; D013076:Sperm Count; D020554:Sperm Injections, Intracytoplasmic / Q000379:methods*; D013081:Sperm Motility; D013094:Spermatozoa / Q000502:physiology