title: Platelet-derived growth factor/insulin-like growth factor-1 combination and bone regeneration around implants placed into extraction sockets: a histometric study in dogs.
abstract: This study evaluated, by histometric analysis, the wound healing process of bone around implants placed into extraction sockets with or without the concurrent application of a combination of platelet-derived growth factor (PDGF) and insulin-like growth factor (IGF-1). Mandibular premolars were removed, and 32 implants were inserted in eight dogs. Before insertion, two implants received a single application of 5 micrograms/mL of PDGF and IGF-1 delivered in 0.10 mL of 4% methylcellulose gel or 0.10 mL of 4% methylcellulose gel only as a control. To label regenerated bone, a 2% calcein green solution was administered by intramuscular injection at 0, 7, 15, 30, 45, 60, and 75 days after implant insertion. Three, 8, and 12 weeks after implant insertion, undecalcified sections were obtained, and the degree of bone contact with the implant surface, the bone area, and the intensity of bone labeling were measured into the limits of the threads of the implant. Greater extension of bone/implant contact (paired t test, P < .05) was observed in test (22.4% +/- 13.7%) than in control (17.2% +/- 13.6%) implants at 3 weeks. One-way ANOVA revealed a higher intensity of bone labeling (P < .05) at 3 weeks than at 12 weeks (127.8 +/- 42.59 and 56.7 +/- 26.34, gray scale values) for test implants. Within the limits of the present study, it was concluded that the combination of PDGF/IGF-I actively took part in the initial phase of bone repair.
authors: Dept. of Prosthodontics and Periodontics, Division of Periodontics, School of Dentistry at Piracicaba, UNICAMP, São Paulo, Brazil.
authors: C M
authors: CM
authors: Stefani
authors: M A
authors: MA
authors: Machado
authors: E A
authors: EA
authors: Sallum
authors: A W
authors: AW
authors: Sallum
authors: S
authors: S
authors: Toledo
authors: F H
authors: H
authors: Nociti
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001641:Bicuspid / Q000601:surgery; D001861:Bone Regeneration / Q000187:drug effects*; D003758:Dental Implantation, Endosseous; D015921:Dental Implants; D004285:Dogs; D004338:Drug Combinations; D005452:Fluoresceins; D005456:Fluorescent Dyes; D005500:Follow-Up Studies; D005782:Gels; D007334:Insulin-Like Growth Factor I / Q000008:administration & dosage / Q000627:therapeutic use*; D008334:Mandible / Q000187:drug effects* / Q000473:pathology / Q000601:surgery; D008747:Methylcellulose; D016348:Osseointegration; D010982:Platelet-Derived Growth Factor / Q000008:administration & dosage / Q000627:therapeutic use*; D011897:Random Allocation; D013223:Statistics as Topic; D013499:Surface Properties; D014081:Tooth Extraction; D020390:Tooth Socket / Q000187:drug effects* / Q000473:pathology / Q000601:surgery; D014945:Wound Healing
chemical_list: D015921:Dental Implants; D004338:Drug Combinations; D005452:Fluoresceins; D005456:Fluorescent Dyes; D005782:Gels; D010982:Platelet-Derived Growth Factor; C083098:calcein green; D007334:Insulin-Like Growth Factor I; D008747:Methylcellulose