Characterization and distribution of neuropeptide Y in the brain of a caecilian amphibian.
Neuropeptide Y (NPY) from the brain of an amphibian from the order Gymnophiona (the caecilian, Typhlonectes natans) was characterized. We cloned a 790 base pair cDNA encoding the caecilian NPY precursor. The open reading frame consisted of 291 bases, indicating an NPY precursor of 97 amino acids. Both deduced and isolated NPY primary structures were Tyr-Pro-Ser-Lys-Pro-Asp-Asn-Pro-Gly-Glu(10)-Asp-Ala-Pro-Ala-Glu-Asp-Met-Ala-Lys-Tyr(20)-Tyr-Ser-Ala-Leu-Arg-His-Tyr-Ile-Asn-Leu(30)-Ile-Thr-Arg-Gln-Arg-Tyr. NH2. In caecilian brain, we observed NPY immunoreactive cells within the medial pallium, basal forebrain, preoptic area, midbrain tegmentum and trigeminal nucleus. The prevalence of preoptic and hypothalamic terminal field staining supports the hypothesis that NPY controls pituitary function in this caecilian.
Ebersole
T J
TJ
Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 46556, USA.
Conlon
J M
JM
Goetz
F W
FW
Boyd
S K
SK
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry; D000663:Amphibians; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism* / Q000473:pathology; D002851:Chromatography, High Pressure Liquid; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D009478:Neuropeptide Y / Q000096:biosynthesis* / Q000737:chemistry* / Q000235:genetics; D016366:Open Reading Frames; D013997:Time Factors; D014018:Tissue Distribution
D000596:Amino Acids; D018076:DNA, Complementary; D009478:Neuropeptide Y