Vaccination with the glycoprotein D gene of pseudorabies virus delivered by nonpathogenic Escherichia coli elicits protective immune responses.
Attenuated intracellular bacteria, such as Salmonella and Shigella, have been exploited to act as gene delivery vectors. In this study, we report that nonpathogenic, live Escherichia coli can be used for the delivery of DNA vaccines in vivo, leading to generation of immune responses against plasmid-encoded foreign antigens. The pseudorabies virus (PrV) DNA vaccine carrying the glycoprotein D (gD) gene delivered by E. coli was able to induce protective immune responses in mice against a lethal PrV challenge. Co-delivery of E. coli carrying plasmid DNA encoding prothymosin alpha enhanced cellular immune responses to the PrV DNA vaccine delivered by E. coli. Our results suggest that nonpathogenic E. coli may be used as a vector for DNA vaccines in veterinary uses.
Shiau
A L
AL
Department of Microbiology, National Cheng Kung University Medical College, 701, Tainan, Taiwan.
Chu
C Y
CY
Su
W C
WC
Wu
C L
CL
D016475:3T3 Cells; D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000818:Animals; D004926:Escherichia coli / Q000235:genetics / Q000472:pathogenicity; D005260:Female; D005814:Genes, Viral; D005822:Genetic Vectors; D011558:Herpesvirus 1, Suid / Q000235:genetics* / Q000276:immunology / Q000472:pathogenicity; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D011498:Protein Precursors / Q000235:genetics / Q000276:immunology; D011557:Pseudorabies / Q000276:immunology / Q000517:prevention & control*; D022421:Pseudorabies Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D013552:Swine; D013553:Swine Diseases / Q000276:immunology / Q000517:prevention & control; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D013947:Thymosin / Q000031:analogs & derivatives / Q000235:genetics / Q000276:immunology; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D014759:Viral Envelope Proteins / Q000235:genetics* / Q000276:immunology
D000276:Adjuvants, Immunologic; D011498:Protein Precursors; D022421:Pseudorabies Vaccines; D019444:Vaccines, DNA; D014759:Viral Envelope Proteins; C040782:prothymosin alpha; C042966:pseudorabies virus glycoproteins; D013947:Thymosin