title: Alteration of cyclin D1 and CDK4 gene in carcinoma of uterine cervix.
abstract: Amplification and overexpression of cyclin D1 and CDK4 genes in cervical carcinoma were studied by semi-quantitative differential polymerase chain reaction assay and an immunostaining technique, respectively. Amplifications of cyclin D1 and CDK4 genes were found in 24% (27/113) and 26% (29/112) of tumors, respectively. Overexpression of cyclin D1 and CDK4 was demonstrated in 32% (21/66) and 73% (45/62) of tumors, respectively. No tumor showed CDK4 gene mutation on single strand conformational polymorphism. Sixteen percent (8/49) of the tumor specimens showed neither amplification nor overexpression. Disease stage, tumor grade and overexpression of cyclin D1 were found to be independent poor prognostic factors in cervical carcinoma.
authors: Department of Obstetrics and Gynaecology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, N.T. Kowloon, Hong Kong, China. thcheung@cuhk.edu.hk
authors: T H
authors: TH
authors: Cheung
authors: M M
authors: MM
authors: Yu
authors: K W
authors: KW
authors: Lo
authors: S F
authors: SF
authors: Yim
authors: T K
authors: TK
authors: Chung
authors: Y F
authors: YF
authors: Wong
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D019938:Cyclin D1 / Q000096:biosynthesis / Q000235:genetics*; D018844:Cyclin-Dependent Kinases / Q000096:biosynthesis / Q000235:genetics*; D005260:Female; D005784:Gene Amplification; D016161:Genes, Retinoblastoma; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis; D016019:Survival Analysis; D002583:Uterine Cervical Neoplasms / Q000235:genetics*
chemical_list: D019938:Cyclin D1; D018844:Cyclin-Dependent Kinases