title: Steam sterilisation of vesicular phospholipid gels.
abstract: Vesicular phospholipid gels (VPGs), highly concentrated phospholipid dispersions of semisolid consistency and vesicular morphology are under investigation as potential implantable depots for sustained release of drugs and as intermediates for subsequent dilution into 'conventional' liposome dispersions. It was investigated here if VPGs can be steam sterilised. VPGs prepared from 400 mg/g egg-phosphatidylcholine by high-pressure homogenisation retained their vesicular structure but showed a slight increase in vesicle size (freeze-fracture electron microscopy). However, autoclaving slowed down both, the in vitro release of the hydrophilic marker carboxyfluorescein and vesicles from VPGs. This was assumed to be due to bigger vesicle sizes and corresponding increase in packing density of the vesicular matrix. Upon dilution into a liposome dispersion both negative staining electron microscopy and dynamic laser light scattering analysis confirmed a distinct increase in liposome size, mainly due to fusion of small (20 nm) vesicles with unfavourable curvature. This was consistent with the observed increase in encapsulation efficiency of carboxyfluorescein. Phospholipid hydrolysis during autoclaving was negligible with lysophosphatidylcholine formation of less than 2% (thin layer chromatography). Despite significant change of their morphological and functional properties during autoclaving VPGs retained their main characteristics, such as vesicular structure, sustained release and dilutability to liposome dispersions, and are, therefore, considered as autoclavable.
authors: Institutt for Farmasi, Avd. Galenisk Farmasi, Universitetet i Tromsø, N-9037, Tromsø, Norway.
authors: C
authors: C
authors: Tardi
authors: M
authors: M
authors: Drechsler
authors: K H
authors: KH
authors: Bauer
authors: M
authors: M
authors: Brandl
mesh_terms: D005452:Fluoresceins / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D005782:Gels; D006868:Hydrolysis; D008081:Liposomes; D010316:Particle Size; D010713:Phosphatidylcholines / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry*; D013227:Steam; D013242:Sterilization / Q000379:methods
chemical_list: D005452:Fluoresceins; D005456:Fluorescent Dyes; D005782:Gels; D008081:Liposomes; D010713:Phosphatidylcholines; D010743:Phospholipids; D013227:Steam; C024098:6-carboxyfluorescein