Quantitative HPLC analysis of acids in Brazilian cachaças and various spirits using fluorescence detection of their 9-anthrylmethyl esters.
An analytical procedure for the separation and quantification of 17 short-chain, medium-chain, and long-chain acids in cachaças and various spirits has been developed involving C18 solid phase extraction, derivatization with 9-anthryldiazomethane, and reverse phase HPLC using fluorescence detection. The limit of detection was between 5 and 15 fmol, whereas the recovery of nonanoic acid as internal standard was >95%. Relative standard deviation values for reproducibility were between 0.09 and 20.4%, and repeatability was between 0.05 and 11.3%.
Ferreira Do Nascimento
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Instituto de Química de São Carlos, Departamento de Química e Física Molecular, Universidade de São Paulo, Caixa Postal 780, 13560-970 São Carlos, SP, Brazil.
Rodrigues Cardoso
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De Keukeleire
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dos Santos Lima-Neto
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Wagner Franco
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D000434:Alcoholic Beverages / Q000032:analysis*; D001938:Brazil; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005453:Fluorescence; D015203:Reproducibility of Results; D013997:Time Factors