The 3' --> 5' exonuclease of T4 DNA polymerase removes premutagenic alkyl mispairs and contributes to futile cycling at O6-methylguanine lesions.
We have studied the processing of O(6)-methylguanine (m6G)-containing oligonucleotides and N-methyl-N-nitrosourea (MNU)-treated DNA templates by the 3' --> 5' exonuclease of T4 DNA polymerase. In vitro biochemical analyses demonstrate that the exonuclease can remove bases opposite a defined m6G lesion. The efficiency of excision of a terminal m6G.T was similar to that of m6G.C, and both were excised as efficiently as a G.T substrate. Partitioning assays between the polymerase and exonuclease activities, performed in the presence of dNTPs, resulted in repeated incorporation and excision events opposite the m6G lesion. This idling produces dramatically less full-length product, relative to natural substrates, indicating that the 3' --> 5' exonuclease may contribute to DNA synthesis inhibition by alkylating agents. Genetic data obtained using an in vitro herpes simplex virus-thymidine kinase assay support the inefficiency of the exonuclease as a "proofreading" activity for m6G, since virtually all mutations produced by the native enzyme using MNU-treated templates were G --> A transitions. Comparison of MNU dose-response curves for exonuclease-proficient and -deficient forms of T4 polymerase reveals that the exonuclease efficiently removes 50-86% of total premutagenic alkyl mispairs. We propose that idling of exonuclease-proficient polymerases at m6G lesions during repair DNA synthesis provides the biochemical explanation for cellular cytotoxicity of methylating agents.
Khare
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Gittlen Cancer Research Institute, Department of Pathology and the Department of Biochemistry and Molecular Biology, Pennsylvania State University College of Medicine, 500 University Dr., Hershey, Pennsylvania 17033, USA.
Eckert
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D000477:Alkylating Agents / Q000494:pharmacology; D020137:Base Pair Mismatch; D001483:Base Sequence; D004260:DNA Repair; D004259:DNA-Directed DNA Polymerase; D005090:Exodeoxyribonucleases / Q000502:physiology; D006147:Guanine / Q000031:analogs & derivatives / Q000378:metabolism*; D008770:Methylnitrosourea / Q000494:pharmacology; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009838:Oligodeoxyribonucleotides / Q000378:metabolism; D015219:Substrate Cycling; D013379:Substrate Specificity; D013698:Templates, Genetic; D014764:Viral Proteins / Q000502:physiology*
D000477:Alkylating Agents; D009838:Oligodeoxyribonucleotides; D014764:Viral Proteins; C069983:gene 43 protein, Enterobacteria phage T4; D006147:Guanine; D008770:Methylnitrosourea; C008449:O-(6)-methylguanine; D004259:DNA-Directed DNA Polymerase; D005090:Exodeoxyribonucleases