Putrescine, DNA, RNA and protein contents in human uterine, breast and rectal cancer.
OBJECTIVE
To find out the status of DNA, RNA and protein in human uterine, ovarian, breast and rectal carcinoma.


METHODS
In this prospective study, patients of age group between late thirties and late fifties suffering from uterine, ovarian, breast and rectal cancer were taken as subjects of the present study. The total number of cases studied for each cases was ten. Pieces of human carcinomatous tissues of above mentioned cases were taken along with surrounding normal tissues. From the tissue samples, putrescine is separated by the method of Herbst et al, DNA analysed by Diphenylamine method, RNA by Orcinol method and protein by Biuret method.


RESULTS
Tissue content of putrescine rises simultaneously with that of DNA, RNA and protein in carcinomatous growths as above in comparison to their respective adjacent normal tissue, the differences being statistically highly significant.


CONCLUSIONS
Increase in DNA, RNA and protein concentration may be a pre-requisite for increased synthesis of putrescine in carcinomatous tissue and thereby the concentration of other di- and poly-amines.
Bandopadhyay
M
M
Department of Medicine, University College of Medicine, Dr. B. C. Roy Postgraduate Institute of Basic Medical Sciences, Calcutta-700 020, India.
Ganguly
A K
AK
D000328:Adult; D014408:Biomarkers, Tumor / Q000032:analysis*; D001707:Biopsy, Needle; D001943:Breast Neoplasms / Q000737:chemistry; D046508:Culture Techniques; D004273:DNA, Neoplasm / Q000032:analysis*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D015513:Oncogene Proteins / Q000032:analysis*; D010051:Ovarian Neoplasms / Q000737:chemistry; D011336:Probability; D011446:Prospective Studies; D011700:Putrescine / Q000032:analysis*; D012334:RNA, Neoplasm / Q000032:analysis*; D012004:Rectal Neoplasms / Q000737:chemistry; D012680:Sensitivity and Specificity; D013053:Spectrophotometry; D014594:Uterine Neoplasms / Q000737:chemistry
D014408:Biomarkers, Tumor; D004273:DNA, Neoplasm; D015513:Oncogene Proteins; D012334:RNA, Neoplasm; D011700:Putrescine