Malignant fibrous histiocytoma (MFH) cells and macrophages/histiocytes have a common antigen recognized by a monoclonal antibody risen against a rat MFH-derived cloned cell line.
A monoclonal antibody (B9) was generated by using a rat malignant fibrous histiocytoma (MFH)-derived cloned cell line (MT-8) as the immunogen. Immunohistochemically, B9 reacted specifically with a cytoplasmic antigen of MT-8 cells. Furthermore, B9 immunolabeled another MFH-derived cloned cells (MT-9) and histiocytic sarcoma cells, as well as macrophages/histiocytes in normal and diseased tissues of rats. These findings suggest the presence of a common antigen recognized by B9 between MFH cells and macrophages/histiocytes. This suggests that MFH cells may express histiocytic nature.
Kumagai
D
D
Veterinary Teaching Hospital, College of Agriculture, Osaka Prefecture University, Sakai, Japan.
Yamate
J
J
Tajima
T
T
Kuwamura
M
M
Kotani
T
T
Sakuma
S
S
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000951:Antigens, Neoplasm / Q000276:immunology* / Q000502:physiology; D004067:Digestive System Neoplasms / Q000276:immunology / Q000473:pathology; D018219:Histiocytoma, Benign Fibrous / Q000276:immunology* / Q000473:pathology; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000276:immunology / Q000473:pathology; D008264:Macrophages / Q000276:immunology* / Q000473:pathology; D051381:Rats; D011916:Rats, Inbred F344; D013160:Splenic Neoplasms / Q000276:immunology / Q000473:pathology; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm