Electroosmosis injection of blood serum into biocompatible microcapillary chip fabricated on quartz plate.
A chip which allows the detection of various human health markers from a trace amount of blood has been studied. As a goal, a microcapillary with a 30 x 30 microm cross-section was fabricated using all-dry etching technologies on a 2 x 2 cm SiO2 chip. The coating of the biocompatible 2-methacryloyloxyethylphosphorylcholine (MPC) polymer on the inner quartz wall of the microcapillary demonstrated a sufficiently long adsorption suppression of proteins in the serum on the quartz surface, while rapid stopping occurred for serum injected into the microcapillary with a bare quartz surface. The latter rapid stopping corresponded well to fast electroosmosis flow due to the negatively increasing zeta-potential by the adsorption of proteins on the quartz surface. The electroosmosis pump arranged a downstream of the microcapillary was also developed to inject serum into it. As a preliminary application, a given concentration-standard solution was injected into the ion-sensitive field-effect transistor (ISFET) embedded in the chip, employing the electroosmosis pump arranged downstream of the sensor position. Hence, the pH and Na+ and K+ cation concentrations were measured.
Oki
A
A
Department of Materials Science, The University of Tokyo, Hongo, Japan. oki@micro.mm.t.u-tokyo.ac.jp
Adachi
S
S
Takamura
Y
Y
Ishihara
K
K
Ogawa
H
H
Ito
Y
Y
Ichiki
T
T
Horiike
Y
Y
D001774:Blood Chemical Analysis / Q000295:instrumentation*; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D004867:Equipment Design; D006801:Humans; D008422:Materials Testing; D008689:Methacrylates / Q000737:chemistry*; D008832:Microchemistry / Q000295:instrumentation*; D008904:Miniaturization; D009995:Osmosis; D010767:Phosphorylcholine / Q000031:analogs & derivatives* / Q000737:chemistry*; D011791:Quartz; D013048:Specimen Handling / Q000295:instrumentation; D017550:Spectroscopy, Fourier Transform Infrared
D020099:Coated Materials, Biocompatible; D008689:Methacrylates; D010767:Phosphorylcholine; D011791:Quartz; C070638:2-methacryloyloxyethyl phosphorylcholine