"Classic" myelin basic proteins are expressed in lymphoid tissue macrophages.
"Classic" myelin basic proteins (MBPs) are demonstrated in lymph nodes of SJL mice by western blot and RT-PCR. Interestingly, expression of these "classic" MBPs was increased during the late relapsing phase of adoptive experimental autoimmune encephalomyelitis (EAE). When splenocytes from SJL mice were separated into macrophage versus B lymphocyte-enriched populations, intact MBP isoforms were demonstrated in the macrophage-enriched population while undetectable in the B lymphocyte-enriched population. RT-PCR demonstrated "classic" MBP transcripts in splenic macrophages, as well as in a macrophage cell line (RAW). The expression of "classic" MBPs in lymphoid tissue macrophages raises the possibility that MBP-specific T cells may be exposed to autoantigen outside the central nervous system (CNS).
Liu
H
H
UCLA Department of Neurology, Reed Neurological Research Center, Los Angeles, CA 90095, USA.
MacKenzie-Graham
A J
AJ
Palaszynski
K
K
Liva
S
S
Voskuhl
R R
RR
D000818:Animals; D002460:Cell Line; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000378:metabolism / Q000473:pathology; D005260:Female; D008198:Lymph Nodes / Q000166:cytology / Q000378:metabolism; D008221:Lymphoid Tissue / Q000166:cytology / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D008815:Mice, Inbred Strains; D004676:Myelin Basic Protein / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D013154:Spleen / Q000166:cytology / Q000378:metabolism
D004676:Myelin Basic Protein; D012333:RNA, Messenger