Expression of calretinin in human mesothelioma cell lines and cell cycle analysis by flow cytometry.
Human mesothelioma cell lines were studied concerning the expression of the calcium-binding protein calretinin (CR), and the relation of the DNA index to their cell cycle. The results obtained for cell lines with different morphological characteristics, were compared to those from human mesothelial cells transfected with SV40 to escape senescence. Immunocytochemical expression of calretinin (confirmed by immunoblot) was observed in all mesothelioma cell lines but not in the control cells. It is suggested that calretinin is active in the first steps of carcinogenesis in all human mesotheliomas and during several stages in the evolution towards malignancy.
Saydan
N
N
Institute of Histology and General Embryology, University of Fribourg, Perolles, CH-1705 Fribourg, Switzerland.
Salicio
V
V
Cappelli-Gotzos
B
B
Gotzos
V
V
D015153:Blotting, Western; D064032:Calbindin 2; D002453:Cell Cycle; D002461:Cell Line, Transformed; D002467:Cell Nucleus / Q000235:genetics; D048429:Cell Size; D002472:Cell Transformation, Viral; D004273:DNA, Neoplasm / Q000032:analysis; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D008654:Mesothelioma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D008940:Mitotic Index; D010641:Phenotype; D064030:S100 Calcium Binding Protein G / Q000276:immunology / Q000378:metabolism*
C576764:CALB2 protein, human; D064032:Calbindin 2; D004273:DNA, Neoplasm; D064030:S100 Calcium Binding Protein G