Screening and search for novel inhibitors of DD-peptidase 64-575.
The aim of the work was search for inhibitors of PBPs (penicillin binding proteins, DD-carboxypeptidase/transpeptidase, DD-peptidase). Between 141 tested Streptomyces strains, 25% showed activity of inhibitors production. The inhibitors were produced by selected Streptomyces strains (NIH Culture Collection). The culture supernatants of Streptomyces rimosus B PZH (inhibitor B) and Streptomyces rimosus NRRL 2234 (inhibitor 2234) exhibited the highest inhibition activity. Both inhibitors were purified by the use of: anion exchange chromatography and reversed phase chromatography (HPLC). Inhibitor B is a gamma-lactam compound and inhibitor 2234 is a beta-lactam.
Rozbicka
B
B
Department of Actinomycetes and Fungi Imperfecti, National Institute of Hygiene (NIH), 24 Chocimska Str., 00-791 Warsaw, Poland.
Kurzatkowski
W
W
D000900:Anti-Bacterial Agents / Q000302:isolation & purification* / Q000494:pharmacology; D002268:Carboxypeptidases / Q000037:antagonists & inhibitors*; D002851:Chromatography, High Pressure Liquid; D004926:Escherichia coli / Q000187:drug effects; D007769:Lactams; D011480:Protease Inhibitors / Q000302:isolation & purification*; D046929:Serine-Type D-Ala-D-Ala Carboxypeptidase; D013211:Staphylococcus aureus / Q000187:drug effects; D013302:Streptomyces / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D007769:Lactams; D011480:Protease Inhibitors; D002268:Carboxypeptidases; D046929:Serine-Type D-Ala-D-Ala Carboxypeptidase