title: Detection of messenger RNA in leukocytes or plasma of patients with chronic myeloid leukemia.
abstract: We investigated the presence of free mRNA in the plasma of patients with chronic myeloid leukemia (CML), through RT-PCR analysis of G3PDH, a metabolism gene. We also analysed the presence of mRNA for HLM, a human oxysterol-binding protein homologue recently described as a potential marker for blood dissemination of solid tumors. Our results showed the presence of metabolism G3PDH mRNA in the plasma of 5/11 (45%) CML patients studied but HLM mRNA was not detected in any of the plasma studied. HLM mRNA was detected in the leukocytes of 4/5 (80%) CML patients. This work reports for the first time free mRNA in the plasma of CML patients. Our results also suggest that the detection of HLM could be a potential molecular marker for the follow-up in hematological malignancies.
authors: Laboratório de Controle da Expressão Genica, Instituto de Biofísica Carlos Chagas Filho, UFRJ, Centro de Ciências da Saude (CCS), Ilha do Fundão, Rio de Janeiro, RJ, CEP 21949-900, Brazil.
authors: N H
authors: NH
authors: Silva
authors: G
authors: G
authors: Pimenta
authors: W A
authors: WA
authors: Pulcheri
authors: M V
authors: MV
authors: Fournier
authors: N
authors: N
authors: Spector
authors: M G
authors: MG
authors: da Costa Carvalho
mesh_terms: D017931:DNA Primers / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000097:blood / Q000235:genetics*; D007962:Leukocytes / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis* / Q000097:blood; D012334:RNA, Neoplasm / Q000097:blood / Q000235:genetics*; D011987:Receptors, Steroid / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000098825:Oxysterol Binding Proteins
chemical_list: D017931:DNA Primers; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D011987:Receptors, Steroid; D000098825:Oxysterol Binding Proteins; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases