title: Use of bacterially expressed EBNA-1 protein cloned from a nasopharyngeal carcinoma (NPC) biopsy as a screening test for NPC patients.
abstract: EBV serological tests have been used for many years as accessory diagnostic predictors of nasopharyngeal carcinoma (NPC). To increase the sensitivity and specificity of the NPC detection rate, a novel enzyme-linked immunosorbent assay (ELISA) was established using a bacterially-expressed GST-EBNA-1 protein, containing the EBNA-1 sequence cloned from an NPC patient. Serum samples were collected from age- and gender-matched patients with NPC, community control subjects and hospital control patients and tested using this ELISA. The positivity rates were 78.7% (247/314) in NPC, 11.5% (28/244) in hospital controls and 3.8% (10/263) in the community control group. These serum samples were also tested for IgA anti-VCA antibodies and their ability to neutralize EBV DNase and the sensitivities of the anti-VCA antibody and DNase-neutralization tests also were analyzed. The optimum combination is VCA plus EBNA-1, which can identify 92.5% (287/310) of NPC patients, and shows a specificity of 92.7% (242/261) for normal individuals.
authors: Graduate Institute of Microbiology, College of Medicine, National Taiwan University, Taipei, Taiwan.
authors: M R
authors: MR
authors: Chen
authors: M Y
authors: MY
authors: Liu
authors: S M
authors: SM
authors: Hsu
authors: C C
authors: CC
authors: Fong
authors: C J
authors: CJ
authors: Chen
authors: I H
authors: IH
authors: Chen
authors: M M
authors: MM
authors: Hsu
authors: C S
authors: CS
authors: Yang
authors: J Y
authors: JY
authors: Chen
mesh_terms: D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral / Q000276:immunology; D001706:Biopsy; D036022:Capsid Proteins; D003001:Cloning, Molecular; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D019309:Epstein-Barr Virus Nuclear Antigens / Q000276:immunology*; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood; D016555:Matched-Pair Analysis; D009303:Nasopharyngeal Neoplasms / Q000175:diagnosis* / Q000821:virology; D009500:Neutralization Tests; D010957:Plasmids; D011993:Recombinant Fusion Proteins / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D012698:Serologic Tests; D013624:Taiwan
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D036022:Capsid Proteins; D000939:Epitopes; D019309:Epstein-Barr Virus Nuclear Antigens; C041788:Epstein-Barr viral capsid antigen; D007070:Immunoglobulin A; D011993:Recombinant Fusion Proteins; C417029:EBV-encoded nuclear antigen 1