title: Identification of a novel human fibroblast growth factor and characterization of its role in oncogenesis.
abstract: The fibroblast growth factor (FGF) family of signaling molecules has been implicated in normal developmental and physiological processes, as well as in human malignancy. Using a homology-based genomic DNA mining process, we identified a human gene encoding a novel member of the FGF family, that we designate FGF-20. The FGF-20 cDNA was isolated, and its sequence confirmed the gene prediction. FGF-20 is expressed in normal brain, particularly the cerebellum, and in some cancer cell lines. Recombinant FGF-20 protein induces DNA synthesis in a variety of cell types and is recognized by multiple FGF receptors. Ectopic expression of FGF-20 in NIH 3T3 cells renders the cells transformed in vitro and tumorigenic in nude mice. These results underscore the utility of mining genomic DNA databases and reveal FGF-20 to be a novel oncogene that may play a role in human cancer.
authors: CuraGen Corporation, New Haven, Connecticut 06511, USA. mjeffers@curagen.com
authors: M
authors: M
authors: Jeffers
authors: R
authors: R
authors: Shimkets
authors: S
authors: S
authors: Prayaga
authors: F
authors: F
authors: Boldog
authors: M
authors: M
authors: Yang
authors: C
authors: C
authors: Burgess
authors: E
authors: E
authors: Fernandes
authors: B
authors: B
authors: Rittman
authors: J
authors: J
authors: Shimkets
authors: W J
authors: WJ
authors: LaRochelle
authors: H S
authors: HS
authors: Lichenstein
mesh_terms: D016475:3T3 Cells / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002455:Cell Division / Q000187:drug effects / Q000235:genetics; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D004247:DNA / Q000096:biosynthesis / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D005346:Fibroblast Growth Factors / Q000235:genetics / Q000494:pharmacology / Q000502:physiology*; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D017468:Receptors, Fibroblast Growth Factor / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity; D014162:Transfection; D014981:Xenopus
chemical_list: D018076:DNA, Complementary; C416003:FGF20 protein, human; C498812:Fgf20 protein, mouse; D012333:RNA, Messenger; D017468:Receptors, Fibroblast Growth Factor; D011994:Recombinant Proteins; D005346:Fibroblast Growth Factors; D004247:DNA