Effect of a combined administration of 5-fluorouracil and medroxyprogesterone acetate on pyrimidine nucleoside phosphorylases and thymidylate synthetase in 7,12-dimethylbenz.
The effect of a combined therapy of medroxyprogesterone acetate (MPA) and 5-fluorouracil (5-FU) on tumor size, pyrimidine nucleoside phosphorylase (PyNPase) activity, and thymidylate synthetase (TS) activity was examined in Sprague-Dawley (SD) rats with 7,12-dimethylbenz[a]anthracene (DMBA)-induced mammary tumors. MPA augmented the antitumor activity of 5-FU and protected against body weight-loss due to 5-FU administration. PyNPase activity of both the MPA group and the MPA+5-FU group tended to increase compared with that of the 5-FU alone group. TS inhibition levels in the MPA+5-FU group tended to increase compared with those in the 5-FU alone group. These results indicate that MPA tended to augment antitumor activity of 5-FU and to reduce the side effects caused by 5-FU.
Nagasawa
M
M
Second Department of Surgery, Gunma University School of Medicine, Maebashi, Gunma 371-8511, Japan. managa@med.gunma-u.ac.jp
Iino
Y
Y
Koibuchi
Y
Y
Andoh
T
T
Morishita
Y
Y
D015127:9,10-Dimethyl-1,2-benzanthracene; D000818:Animals; D000964:Antimetabolites, Antineoplastic / Q000627:therapeutic use*; D018931:Antineoplastic Agents, Hormonal / Q000627:therapeutic use*; D001835:Body Weight; D004359:Drug Therapy, Combination; D005260:Female; D005472:Fluorouracil / Q000627:therapeutic use*; D008325:Mammary Neoplasms, Experimental / Q000139:chemically induced / Q000188:drug therapy* / Q000201:enzymology; D017258:Medroxyprogesterone Acetate / Q000627:therapeutic use*; D010430:Pentosyltransferases / Q000378:metabolism*; D057845:Pyrimidine Phosphorylases; D051381:Rats; D017207:Rats, Sprague-Dawley; D013940:Thymidylate Synthase / Q000378:metabolism*
D000964:Antimetabolites, Antineoplastic; D018931:Antineoplastic Agents, Hormonal; D015127:9,10-Dimethyl-1,2-benzanthracene; D017258:Medroxyprogesterone Acetate; D013940:Thymidylate Synthase; D010430:Pentosyltransferases; D057845:Pyrimidine Phosphorylases; D005472:Fluorouracil