Competitive uptake of porphyrin and LDL via the LDL receptor in glioma cell lines: flow cytometric analysis.
We examined the simultaneous uptake of porphyrin and (LDL) by four established cell lines of glioma and normal fibroblasts using flow cytometry (FCM). The results indicated porphyrin and LDL showed competitive conjugation with the LDL receptor. These results support the theory of the porphyrin uptake via the LDL receptor.
Shibata
Y
Y
Department of Neurosurgery, University of Tsukuba, Tsukuba, 305-8575, Ibaraki, Japan.
Matsumura
A
A
Yoshida
F
F
Yamamoto
T
T
Nakai
K
K
Nose
T
T
Sakata
I
I
Nakajima
S
S
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001667:Binding, Competitive; D002460:Cell Line; D005347:Fibroblasts / Q000378:metabolism; D005434:Flow Cytometry; D005910:Glioma / Q000378:metabolism*; D006801:Humans; D008077:Lipoproteins, LDL / Q000378:metabolism*; D011166:Porphyrins / Q000378:metabolism*; D051381:Rats; D011973:Receptors, LDL / Q000276:immunology / Q000378:metabolism*; D014357:Trypsin / Q000737:chemistry; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D008077:Lipoproteins, LDL; D011166:Porphyrins; D011973:Receptors, LDL; D014357:Trypsin