Abnormal auditory brainstem responses for mice treated with mercurial compounds: involvement of excessive nitric oxide.
In this paper, we attempted to construct an animal (mouse) model for monitoring the oto-neurotoxicity of mercuric sulfide, comparing its toxicity with the well-known (organic) mercury compound methyl-mercury. Mice were treated with either mercuric sulfide (HgS, 0.1 and 1.0 g/kg per day) or methyl-mercury (MeHg, 0.2, 2.0 and 10 mg/kg per day) by gastric gavage for 7 consecutive days. Analysis of auditory brainstem response (ABR) indicated that significant elevation of the physiological hearing threshold as well as significant prolongation of interwave latency I-V was observed for MeHg -- (2.0 and 0.2 mg/kg per day) or HgS -- (1.0 g/kg per day, but not 0.1 g/kg per day) treated mice. Further, both MeHg- and HgS-treated animals demonstrated a significant prolongation of interwave latency I-V that increased with an increasing mean blood-Hg level. The oto-neurotoxicity of MeHg (2.0 mg/kg per day) persisted to at least 11 weeks subsequent to the cessation of its administration. The toxic effect of HgS, however, disappeared completely 5 weeks subsequent to the cessation of its administration. These results suggest a correlation between the Hg-elicited hearing dysfunction and the availability of mercury in brain tissue. Both inhibition of Na(+)/K(+)-ATPase activity and overproduction of nitric oxide in the brainstem are consistent with an analysis of the physiological hearing threshold and latencies of ABR waveform at all time points throughout the experimental process. Thus, it is proposed that high-dose HgS or MeHg intoxication is associated with a decrease in functional Na(+)/K(+)-ATPase activity in the brainstem of affected animals, this presumably arising via excessive nitric oxide production, and suggesting that brainstem damage may play a role in mercury-induced hearing loss.
Chuu
J J
JJ
Institutes of Toxicology, College of Medicine, National Taiwan University, No. 1, Section 1, Jen-Ai Road, Taipei 10043, Taiwan.
Hsu
C J
CJ
Lin-Shiau
S Y
SY
D000818:Animals; D001933:Brain Stem / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D016057:Evoked Potentials, Auditory, Brain Stem / Q000187:drug effects*; D006311:Hearing Disorders / Q000139:chemically induced*; D008297:Male; D008628:Mercury / Q000032:analysis; D017669:Mercury Compounds / Q000378:metabolism / Q000633:toxicity*; D008767:Methylmercury Compounds / Q000378:metabolism / Q000633:toxicity; D051379:Mice; D009569:Nitric Oxide / Q000096:biosynthesis*; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism
D017669:Mercury Compounds; D008767:Methylmercury Compounds; D009569:Nitric Oxide; D000254:Sodium-Potassium-Exchanging ATPase; D008628:Mercury; C004925:methylmercuric chloride; C034211:cinnabar