The use of bone morphogenetic protein gene therapy in craniofacial bone repair.
Bone morphogenetic proteins (BMPs) are capable of inducing endochondral bone formation when applied on biologic carriers in numerous mammalian in vivo assay systems. Bone morphogenetic protein gene therapy is also currently being developed to promote osteogenesis for clinical indications such as spinal fusions, craniofacial bone loss, and osteoporosis. In this study, critical-sized mandibular defects were treated with a control adenoviral vector (Ad-beta-gal), a BMP-2 adenoviral vector (Ad-BMP-2), or a BMP-9 adenoviral vector (Ad-BMP-9). Gross tissue examination, radiographic analysis, and histologic analysis demonstrated significant bony healing in the BMP treated groups compared to controls. Osteogenesis was limited to the bony defect, without extension into the surrounding soft tissues. The study suggests that with further development, BMP gene therapy may be potentially useful for repair of bony defects in the craniofacial region.
Alden
T D
TD
Department of Neurosurgery, University of Virginia, Box 212, Charlottesville, VA 22908, USA.
Beres
E J
EJ
Laurent
J S
JS
Engh
J A
JA
Das
S
S
London
S D
SD
Jane
J A
JA
Hudson
S B
SB
Helm
G A
GA
D000818:Animals; D001853:Bone Marrow / Q000000981:diagnostic imaging / Q000473:pathology; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000235:genetics* / Q000627:therapeutic use; D001861:Bone Regeneration; D003587:Cytomegalovirus / Q000235:genetics; D005500:Follow-Up Studies; D015316:Genetic Therapy; D005822:Genetic Vectors; D055427:Growth Differentiation Factor 2; D007091:Image Processing, Computer-Assisted; D008334:Mandible / Q000000981:diagnostic imaging / Q000473:pathology; D008336:Mandibular Diseases / Q000000981:diagnostic imaging / Q000473:pathology / Q000628:therapy*; D010012:Osteogenesis; D010024:Osteoporosis / Q000628:therapy; D051381:Rats; D011923:Rats, Nude; D013123:Spinal Fusion; D014057:Tomography, X-Ray Computed; D016212:Transforming Growth Factor beta / Q000235:genetics* / Q000627:therapeutic use; D014945:Wound Healing; D001616:beta-Galactosidase / Q000235:genetics
C525720:Bmp2 protein, rat; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D055427:Growth Differentiation Factor 2; D016212:Transforming Growth Factor beta; D001616:beta-Galactosidase