Genotyping of group A rotavirus samples from Brazilian children by probe hybridization.
The G genotyping of 74 group A rotavirus samples was done by RNA-DNA hybridization (dot-blot) using oligonucleotide probes for the VP7 gene region of the human rotavirus serotypes/genotypes 1, 2, 3 and 4. Thirty-one samples could be genotyped by dot-blot showing the following results: G1 = 16, G4 = 6, G3 = 5, and G2 = 4. The data show circulation of genotypes G1-G4 and the predominance of G1. The knowledge of genotypes provides important information concerning rotavirus circulation in Central Brazil.
Cardoso
D D
DD
Laboratório de Virologia, Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Rua Delenda Rezende Melo, Esquina com 1a Avenida, 74605-050 Goiânia, GO, Brazil. divina@netgo.com.br
Rácz
M L
ML
Azevedo
M S
MS
Martins
R M
RM
Soares
C M
CM
D001938:Brazil; D002648:Child; D003967:Diarrhea / Q000821:virology; D005838:Genotype; D006801:Humans; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D012401:Rotavirus / Q000145:classification / Q000235:genetics*
D015345:Oligonucleotide Probes