In vitro cytotoxicity evaluation of denture adhesive.
OBJECTIVE
The ideal denture adhesive demonstrates a minimal toxicity to the mucosa. The purpose of this study was to evaluate the in vitro cytotoxicity of a new denture adhesive.


METHODS
Samples were prepared and extracted in Dulbecco's Modified Eagle's Medium. The cell viability of human oral fibroblast cells cultivated for 24 hours with DA-containing medium was evaluated by the MTT assay to investigate cell proliferation. Optical densities were measured by a spectrophotometer at 490 nm.


RESULTS
The statistical analysis of results showed that this denture adhesive displayed only slight cytotoxicity.


CONCLUSIONS
The generally favorable in vitro cytotoxicity of the denture adhesive formulation indicates that this product may be efficacious.
Cheng
X R
XR
Department of Prosthodontics, School of Stomatology, Wuhan University, No. 237 Luoyu Road, Wuhan 430079, P.R. China.
Zhao
K
K
Han
G L
GL
D000269:Adhesives / Q000737:chemistry / Q000633:toxicity*; D000293:Adolescent; D000328:Adult; D001672:Biocompatible Materials / Q000737:chemistry / Q000633:toxicity*; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002626:Chemistry, Pharmaceutical; D002648:Child; D004396:Coloring Agents; D003781:Denture Retention; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D010519:Periodontium / Q000166:cytology / Q000187:drug effects*; D013053:Spectrophotometry; D013778:Tetrazolium Salts; D013844:Thiazoles
D000269:Adhesives; D001672:Biocompatible Materials; D004396:Coloring Agents; D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue