Molecular epidemiology of methicillin-resistant Staphylococcus aureus.
Subtyping methicillin- resistant Staphylococcus aureus (MRSA) isolates and tracking nosocomial infections have evolved from phenotypic to genotypic approaches; most laboratories now depend on pulsed-field gel electrophoresis (PFGE). We discuss the limitations of current image-based genotyping methods, including PFGE, and the advantages (including ease of entering data into a database) of using DNA sequence analysis to control MRSA infections in health-care facilities.
Shopsin
B
B
Public Health Research Institute and Department of Microbiology, New York University School of Medicine, New York, New York, USA.
Kreiswirth
B N
BN
D015373:Bacterial Typing Techniques / Q000379:methods; D004269:DNA, Bacterial / Q000032:analysis; D005838:Genotype; D006801:Humans; D016106:Methicillin Resistance; D017422:Sequence Analysis, DNA / Q000379:methods; D013203:Staphylococcal Infections / Q000453:epidemiology / Q000382:microbiology; D013211:Staphylococcus aureus / Q000145:classification / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification
D004269:DNA, Bacterial