Protein refolding for industrial processes.
Inclusion body refolding processes are poised to play a major role in the production of recombinant proteins. Improving renaturation yields by minimizing aggregation and reducing chemical costs are key to the industrial implementation of these processes. Recent developments include solubilization methods that do not rely on high denaturant concentrations and the use of high hydrostatic pressure for simultaneous solubilization and renaturation.
Clark
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Department of Chemical and Biological Engineering, Tufts University, Medford, MA 02155, USA. eliana.clark@tufts.edu
D004220:Disulfides / Q000737:chemistry; D004345:Drug Industry; D002479:Inclusion Bodies / Q000737:chemistry*; D010084:Oxidation-Reduction; D011312:Pressure; D017510:Protein Folding; D020673:Protein Renaturation; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000627:therapeutic use
D004220:Disulfides; D011994:Recombinant Proteins