Long-lasting induction of brain-derived neurotrophic factor is restricted to resistant cell populations in an animal model of status epilepticus.
We have recently characterized an animal model of status epilepticus induced by a single intraseptal injection of kainate. Under these conditions, there is a delayed expanding apoptotic hippocampal and amygdalar cell death. In order to further characterize this animal model, we have performed a detailed time-course analysis of the appearance of cell death, brain-derived neurotrophic factor messenger RNA expression and astroglial and microglial response in different brain areas related to the limbic system. We found a long-lasting delayed apoptotic cell death in the hippocampal formation, amygdala, medial thalamus, dorsal endopiriform nucleus and multiple cortical areas from two to 21 days post-injection. There was a spatiotemporal correlation between the appearance of cell death and induction of brain-derived neurotrophic factor messenger RNA expression in the areas studied, and interestingly this induction was found in non-degenerating cells. We conclude that our animal model of status epilepticus exhibits remarkable features of recurrent seizure activity and provides evidence for a neuroprotective role of brain-derived neurotrophic factor against seizure-induced apoptotic cell death.
Revuelta
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Departamento de Bioquímica, Bromatología, Toxicología y Medicina Legal, Facultad de Farmacia, Universidad de Sevilla, Calle Prof. García González s/n, 41012, Sevilla, Spain.
Castaño
A
A
Venero
J L
JL
Machado
A
A
Cano
J
J
D000818:Animals; D017209:Apoptosis; D001522:Behavior, Animal; D001921:Brain / Q000378:metabolism; D019208:Brain-Derived Neurotrophic Factor / Q000235:genetics / Q000378:metabolism*; D016923:Cell Death; D004195:Disease Models, Animal; D004198:Disease Susceptibility; D018690:Excitatory Amino Acid Agonists; D005260:Female; D007608:Kainic Acid; D009457:Neuroglia / Q000502:physiology; D009474:Neurons / Q000378:metabolism* / Q000502:physiology; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013226:Status Epilepticus / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology* / Q000523:psychology; D013997:Time Factors
D019208:Brain-Derived Neurotrophic Factor; D018690:Excitatory Amino Acid Agonists; D012333:RNA, Messenger; D007608:Kainic Acid