[Extracellular factors of adaptation of Pneudomonas fluorescens batch cultures to adverse conditions].
The culture liquid filtrate of an exponential-phase Pseudomonas fluorescens batch culture added to another P. fluorescens culture at the moment of inoculation was found (1) to prevent or diminish cell adsorption of the flask walls; (2) to enhance the intensity of cell respiration; (3) to shorten the period of adaptation of LB-grown cells to growth in glucose-containing mineral M9 medium; (4) to stimulate bacterial growth at supraoptimum temperature (36 degrees C) and pH values (4.8 and 9.2); and (5) to decrease the death rate of bacteria at the supraoptimum growth temperature. These results were interpreted as indicating that P. fluorescens cultures produce two types of regulatory exometabolites similar to those revealed earlier in Escherichia coli and Bacillus subtilis cultures: the direct-action adaptogenic factor XI capable of increasing bacterial resistance to unfavorable growth conditions (temperature and pH) and factor promoting adaptation to new media. Both factors are presumably low-molecular-weight hydrophilic nonprotein compounds.
Nikolaev
Iu A
IuA
Institute of Microbiology, Russian Academy of Sciences, pr. 60-letiya Oktyabrya 7, k. 2, Moscow, 117811 Russia.
Prosser
J I
JI
Panikov
N S
NS
D000222:Adaptation, Physiological; D003470:Culture Media; D006863:Hydrogen-Ion Concentration; D011551:Pseudomonas fluorescens / Q000502:physiology*; D013696:Temperature
D003470:Culture Media