PCR-SSCP: a simple method for the authentication of grouper (Epinephelus guaza), wreck fish (Polyprion americanus), and Nile perch (Lates niloticus) fillets.
A method of DNA analysis has been developed to verify the authenticity of grouper (Epinephelus guaza), wreck fish (Polyprion americanus), and Nile perch (Lates niloticus) fillets. A short fragment (208 bp) of the mitochondrial 12S rRNA gene was amplified by the polymerase chain reaction and analyzed by single-strand conformation polymorphism to get species-specific patterns of single-stranded DNA (ssDNA). DNA strands were separated by native polyacrylamide gel electrophoresis and visualized by silver staining. Discrimination among the three fish species studied was possible, because each one expressed a specific ssDNA pattern.
Asensio
L
L
Departamento de Nutrición y Bromatología III, Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain.
González
I
I
Fernández
A
A
Rodríguez
M A
MA
Hernández
P E
PE
García
T
T
Martín
R
R
D000818:Animals; D004277:DNA, Single-Stranded / Q000032:analysis; D004587:Electrophoresis, Agar Gel; D010473:Perciformes / Q000145:classification* / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D018807:Polymorphism, Single-Stranded Conformational; D012335:RNA, Ribosomal / Q000235:genetics*; D017747:Seafood / Q000592:standards*; D013045:Species Specificity
D004277:DNA, Single-Stranded; D012335:RNA, Ribosomal; C075526:RNA, ribosomal, 12S