title: Expression, cloning, and immunological analysis of buckwheat (Fagopyrum esculentum Moench) seed storage proteins.
abstract: cDNA of buckwheat (Fagopyrum esculentum Moench) was isolated from immature seeds harvested 14 days after pollination. Two genes, designated FA02 and FA18, were found to encode legumin-like proteins and were expressed during seed development. The deduced amino acid sequence of FA02 was identical to the N-terminal amino acid domain of BW24KD, which was believed to be a major buckwheat allergen (Urisu, A.; Kondo, Y.; Morita, Y.; Yagi, E.; Tsuruta, M.; Yasaki, T.; Yamada, K.; Kuzuya, H.; Suzuki, M.; Titani, K.; Kurosawa, K. Isolation and characterization of a major allergen in buckwheat seeds. In Current Advances in Buckwheat Research; Shinshu University Press: Matsumoto, Japan, 1995; pp 965--974). It was predicted that FA02 would be cleaved to generate two separate components, a 41.3 kDa alpha-subunit and a 21 kDa beta-subunit. Antiserum was raised against the deduced FA02 beta-subunit, and immunoblotting of total protein from buckwheat seeds (F. esculentum M. and Fagopyrum tartaricum Gaertn.) revealed that several groups of proteins reacted with the antiserum. Polypeptides in the 23--25 kDa range displayed the greatest reactivity.
authors: Department of Crop Physiology, Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589, Japan. kaien@res.agr.hokudai.ac.jp
authors: K
authors: K
authors: Fujino
authors: H
authors: H
authors: Funatsuki
authors: M
authors: M
authors: Inada
authors: Y
authors: Y
authors: Shimono
authors: Y
authors: Y
authors: Kikuta
mesh_terms: D000485:Allergens / Q000235:genetics* / Q000276:immunology; D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel; D019612:Fagopyrum / Q000737:chemistry*; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D015151:Immunoblotting; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D012639:Seeds / Q000737:chemistry*
chemical_list: D000485:Allergens; D018076:DNA, Complementary; D010940:Plant Proteins