Molecular cloning and chromosomal localization of human salt-tolerant protein.
We have isolated a novel human cDNA coding for human salt-tolerant protein (HSTP), that is a homologue of the rat salt-tolerant protein (STP) and may contribute to salt-induced hypertension by modulating renal cation transport. The nucleotide sequence (1988bp) of the HSTP cDNA contains an open reading frame encoding a polypeptide comprising 545 amino acids, two residues fewer than the rat STP cDNA. The predicted amino acid sequence exhibits 92% identity to that of the rat protein. HSTP contains predicted coiled-coil domains and Src Homology 3 domain, and shows a high degree of identity to CIP4 (Cdc42 target protein) and human Trip 10 (thyroid-hormone receptor interacting protein). We have mapped the HSTP gene to human chromosome 19 by fluorescence in situ hybridization.
Tsuji
E
E
Second Department of Internal Medicine, School of Medicine, Fukuoka University, Japan. emikot@fukuoka-u.ac.jp
Tsuji
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D002874:Chromosome Mapping; D002888:Chromosomes, Human, Pair 19 / Q000235:genetics; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D006973:Hypertension / Q000209:etiology; D017404:In Situ Hybridization, Fluorescence; D008869:Microtubule-Associated Proteins; D015778:Minor Histocompatibility Antigens; D008969:Molecular Sequence Data; D051381:Rats; D017386:Sequence Homology, Amino Acid; D017673:Sodium Chloride, Dietary / Q000009:adverse effects
D002352:Carrier Proteins; D018076:DNA, Complementary; D008869:Microtubule-Associated Proteins; D015778:Minor Histocompatibility Antigens; D017673:Sodium Chloride, Dietary; C406285:TRIP10 protein, human; C498640:Trip10 protein, rat