title: Identity of dimeric dihydrodiol dehydrogenase as NADP(+)-dependent D-xylose dehydrogenase in pig liver.
abstract: Dimeric dihydrodiol dehydrogenases (DDs, EC 1.3.1.20), which oxidize trans-dihydrodiols of aromatic hydrocarbons to the corresponding catechols, have been molecularly cloned from human intestine, monkey kidney, pig liver, dog liver, and rabbit lens. A comparison of the sequences with the DNA sequences in databases suggested that dimeric DDs constitute a novel protein family with 20 gene products. In addition, it was found that dimeric DD oxidizes several pentoses and hexoses, and the specificity resembles that of NADP(+)-dependent D-xylose dehydrogenase (EC 1.1.1.179) of pig liver. The inhibition of D-xylose dehydrogenase activity in the extracts of monkey kidney, dog liver and pig liver, its co-purification with dimeric DD activity from pig liver, and kinetic analysis of the D-xylose reduction by pig dimeric DD indicated that the two enzymes are the same protein.
authors: Laboratory of Biochemistry, Gifu Pharmaceutical University, 5-6-1 Mitahora-higashi, 502-8585, Gifu, Japan.
authors: S
authors: S
authors: Aoki
authors: S
authors: S
authors: Ishikura
authors: Y
authors: Y
authors: Asada
authors: N
authors: N
authors: Usami
authors: A
authors: A
authors: Hara
mesh_terms: D000429:Alcohol Oxidoreductases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D002021:Buffers; D050260:Carbohydrate Metabolism; D019281:Dimerization; D004285:Dogs; D000882:Haplorhini; D006801:Humans; D066298:In Vitro Techniques; D008099:Liver / Q000201:enzymology*; D008969:Molecular Sequence Data; D009249:NADP / Q000378:metabolism; D010088:Oxidoreductases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D011817:Rabbits; D012492:Salts; D013379:Substrate Specificity; D013552:Swine; D014018:Tissue Distribution
chemical_list: D002021:Buffers; D012492:Salts; D009249:NADP; D010088:Oxidoreductases; D000429:Alcohol Oxidoreductases; C035795:D-xylose dehydrogenase; C048338:trans-1,2-dihydrobenzene-1,2-diol dehydrogenase