title: Aggregation substance-mediated adherence of Enterococcus faecalis to immobilized extracellular matrix proteins.
abstract: Aggregation substance (AS) of Enterococcus faecalis (E. faecalis), a sex pheromone plasmid encoded cell surface protein, mediates the formation of bacterial aggregates, thereby promoting plasmid transfer. The influence of pAD1-encoded AS, Asa1, on binding to immobilized extracellular matrix proteins was studied. The presence of AS increased enterococcal adherence to fibronectin more than eight-fold, to thrombospondin more than four-fold, to vitronectin more than three-fold, and to collagen type I more than two-fold (P<0.001). In contrast, binding to laminin and collagen type IV occurred independently of AS. Adherence of the constitutively AS expressing E. faecalis OG1X(pAM721) to immobilized fibronectin was found to be approximately five times higher than that of Staphylococcus aureus Cowan and approximately 30 times higher than that of Streptococcus bovis. Investigation of strains with various deletions within the structural gene of asa1 suggests that attachment to immobilized fibronectin is mainly mediated by amino acids within the variable region or by neighbouring residues. Thus, AS may promote adherence to injured epithelium and endothelium, where extracellular matrix proteins are exposed, thereby facilitating colonization and infection.
authors: Department of Medical Microbiology and Hygiene, University of Ulm, Ulm, D-89081, Germany. eva.rozdzinski@medizin.uni-ulm.de
authors: E
authors: E
authors: Rozdzinski
authors: R
authors: R
authors: Marre
authors: M
authors: M
authors: Susa
authors: R
authors: R
authors: Wirth
authors: A
authors: A
authors: Muscholl-Silberhorn
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D013293:Enterococcus faecalis / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D005353:Fibronectins / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D010675:Pheromones / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D010957:Plasmids; D011485:Protein Binding; D017384:Sequence Deletion; D014774:Virulence
chemical_list: D001426:Bacterial Proteins; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D010675:Pheromones; C065937:aggregation substance, Enterococcus faecalis