title: Influence of biomaterial surface chemistry on the apoptosis of adherent cells.
abstract: A common component of the foreign-body response to implanted materials is the presence of adherent macrophages that fuse to form foreign-body giant cells (FBGCs). These multinucleated cells have been shown to concentrate the phagocytic and degradative properties of macrophages at the implant surface and are responsible for the damage and failure of the implant. Therefore, the modulation of the presence or actions of macrophages and FBGCs at the material-tissue interface is an extensive area of recent investigations. A possible mechanism to achieve this is through the induction of the apoptosis of adherent macrophages, which results in no inflammatory consequence. We hypothesize that the induction of the apoptosis of biomaterial adherent cells can be influenced by the chemistry of the surface of adhesion. Herein, we demonstrate that surfaces displaying hydrophilic and anionic chemistries induce apoptosis of adherent macrophages at a higher magnitude than hydrophobic or cationic surfaces. Additionally, the level of apoptosis for a given surface is inversely related to that surface's ability to promote the fusion of macrophages into FBGCs. This suggests that macrophages fuse into FBGCs to escape apoptosis.
authors: Department of Pathology, Case Western Reserve University, Cleveland, Ohio, USA.
authors: W G
authors: WG
authors: Brodbeck
authors: M S
authors: MS
authors: Shive
authors: E
authors: E
authors: Colton
authors: Y
authors: Y
authors: Nakayama
authors: T
authors: T
authors: Matsuda
authors: J M
authors: JM
authors: Anderson
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology*; D002448:Cell Adhesion; D002478:Cells, Cultured / Q000187:drug effects* / Q000473:pathology*; D006801:Humans; D008262:Macrophage Activation / Q000187:drug effects; D008264:Macrophages / Q000187:drug effects; D009000:Monocytes; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials