Short term hypercholesterolemia alters N(G)-nitro-L-arginine- and indomethacin-resistant endothelium-dependent relaxation by acetylcholine in rabbit renal artery.
The tension of isolated rings was measured isometrically to compare the N(G)-nitro-L-arginine- and indomethacin-resistant relaxation by acetylcholine (ACh) in the renal artery from normal rabbits and short term hypercholesterolemia rabbits (0.5% cholesterol chow for 5 weeks). ACh-induced relaxation in the renal artery precontracted with phenylephrine was not influenced by cholesterol-enriched chow. However, in comparison with artery from normal rabbits, the N(G)-nitro-L-arginine- and indomethacin-resistant endothelium-dependent relaxation by ACh was significantly enhanced by the chow. The resistant part of ACh-induced relaxation was significantly inhibited when the artery was treated with tetraethylammonium or SKF 525a. Results suggest that short term hypercholesterolemia modulates endothelium-derived hyperpolarizing factor-mediated relaxation in rabbit renal artery.
Honda
H
H
Department of Pharmacology, Tokyo University of Pharmacy and Life Science, Hachioji, Japan. hhonda@ps.toyaku.ac.jp
Moroe
H
H
Fujii
H
H
Arai
K
K
Notoya
Y
Y
Kogo
H
H
D000109:Acetylcholine / Q000494:pharmacology*; D000818:Animals; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000503:physiopathology; D006937:Hypercholesterolemia / Q000503:physiopathology*; D007213:Indomethacin / Q000494:pharmacology*; D009126:Muscle Relaxation / Q000187:drug effects; D019335:Nitroarginine / Q000494:pharmacology*; D011817:Rabbits; D012077:Renal Artery / Q000187:drug effects* / Q000503:physiopathology
D019335:Nitroarginine; D000109:Acetylcholine; D007213:Indomethacin