Alteration of cyclin D1 and CDK4 gene in carcinoma of uterine cervix.
Amplification and overexpression of cyclin D1 and CDK4 genes in cervical carcinoma were studied by semi-quantitative differential polymerase chain reaction assay and an immunostaining technique, respectively. Amplifications of cyclin D1 and CDK4 genes were found in 24% (27/113) and 26% (29/112) of tumors, respectively. Overexpression of cyclin D1 and CDK4 was demonstrated in 32% (21/66) and 73% (45/62) of tumors, respectively. No tumor showed CDK4 gene mutation on single strand conformational polymorphism. Sixteen percent (8/49) of the tumor specimens showed neither amplification nor overexpression. Disease stage, tumor grade and overexpression of cyclin D1 were found to be independent poor prognostic factors in cervical carcinoma.
Cheung
T H
TH
Department of Obstetrics and Gynaecology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, N.T. Kowloon, Hong Kong, China. thcheung@cuhk.edu.hk
Yu
M M
MM
Lo
K W
KW
Yim
S F
SF
Chung
T K
TK
Wong
Y F
YF
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D019938:Cyclin D1 / Q000096:biosynthesis / Q000235:genetics*; D018844:Cyclin-Dependent Kinases / Q000096:biosynthesis / Q000235:genetics*; D005260:Female; D005784:Gene Amplification; D016161:Genes, Retinoblastoma; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis; D016019:Survival Analysis; D002583:Uterine Cervical Neoplasms / Q000235:genetics*
D019938:Cyclin D1; D018844:Cyclin-Dependent Kinases