IL-6 up-regulates CNTF mRNA expression and enhances neurite regeneration.
Interleukin-6 (IL-6) is a neurotrophic cytokine, however, its direct effect on nerve regeneration has not been well characterized. We therefore examined the effect of IL-6 on neurite regeneration using the rat dorsal root ganglion. IL-6 significantly enhanced neurite regeneration from transected nerve terminals. We also examined the mRNA expression of IL-6 family cytokines and their receptors during the regeneration. The mRNA expressions of IL-6, IL-6 receptor, leukemia inhibitory factor (LIF), ciliary neurotrophic factor (CNTF) receptor alpha, and LIF receptor beta showed no significant differences by the addition of IL-6. In contrast, IL-6 enhanced the mRNA expression of gp130 and CNTF. In addition, CNTF significantly increased neurite regeneration when added exogenously. Our data suggest that IL-6 enhanced regeneration via up-regulating CNTF expression.
Shuto
T
T
Department of Neurosurgery, Yokohama City University, School of Medicine, Kanagawa, Japan.
Horie
H
H
Hikawa
N
N
Sango
K
K
Tokashiki
A
A
Murata
H
H
Yamamoto
I
I
Ishikawa
Y
Y
D000818:Animals; D001369:Axons / Q000502:physiology; D019771:Axotomy; D020934:Ciliary Neurotrophic Factor / Q000096:biosynthesis*; D007091:Image Processing, Computer-Assisted; D015850:Interleukin-6 / Q000494:pharmacology*; D009416:Nerve Regeneration / Q000187:drug effects*; D016501:Neurites / Q000187:drug effects*; D017729:Presynaptic Terminals / Q000187:drug effects; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation / Q000187:drug effects*
D020934:Ciliary Neurotrophic Factor; D015850:Interleukin-6; D012333:RNA, Messenger