Effect of Schistosoma mansoni-induced granulomatous inflammation on murine gastrointestinal motility.
In Schistosoma mansoni-infected mice, gastrointestinal transit was measured in vivo and the neuromuscular function of longitudinal muscle strips of inflamed ileum and noninflamed gastric fundus was assessed in vitro. Eight weeks after infection, the ileal wall was acutely inflamed, as shown by a mucosal inflammatory infiltrate, leading to an increase in mucosal thickness, in myeloperoxidase (MPO) activity, and in interleukin (IL)-1beta production. At that time, both gastrointestinal transit and in vitro ileal contractility were normal. Twelve weeks after infection, chronic granulomatous inflammation led to proliferation of the muscle layer and to a further increase in MPO activity, whereas IL-1beta production normalized. Gastrointestinal transit was decreased, whereas in vitro ileal contractility was increased irrespective of the contractile stimulus. In vitro incubation with IL-1beta (10 ng/ml for 60 min) significantly increased ileal contractility only at 8 wk after infection. Indomethacin, tetrodotoxin, and atropine had no differential effect on ileal contractility in controls and infected mice. In vitro contractility of noninflamed gastric fundus was normal both 8 and 12 wk after infection. We conclude that intestinal schistosomiasis 8 wk after infection is associated only with structural changes of the ileum, whereas 12 wk after infection, both structural and functional changes are present. These changes are characterized by increased ileal wall thickness, decreased gastrointestinal transit, and increased smooth muscle contractility restricted to the inflamed gut segment.
Moreels
T G
TG
Division of Gastroenterology, Faculty of Medicine, University of Antwerp, Universiteitsplein 1, B-2610 Wilrijk, Belgium.
De Man
J G
JG
Bogers
J J
JJ
De Winter
B Y
BY
Vrolix
G
G
Herman
A G
AG
Van Marck
E A
EA
Pelckmans
P A
PA
D000818:Animals; D015415:Biomarkers / Q000032:analysis; D015237:Dinoprost / Q000494:pharmacology; D005753:Gastric Mucosa / Q000473:pathology / Q000503:physiopathology; D005769:Gastrointestinal Motility; D005772:Gastrointestinal Transit / Q000502:physiology; D006099:Granuloma / Q000469:parasitology* / Q000503:physiopathology; D007082:Ileum / Q000187:drug effects / Q000473:pathology / Q000503:physiopathology*; D066298:In Vitro Techniques; D007249:Inflammation; D007375:Interleukin-1 / Q000032:analysis / Q000494:pharmacology; D007413:Intestinal Mucosa / Q000473:pathology / Q000503:physiopathology; D008297:Male; D051379:Mice; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects / Q000473:pathology / Q000503:physiopathology*; D009195:Peroxidase / Q000032:analysis; D011189:Potassium Chloride / Q000494:pharmacology; D012016:Reference Values; D012555:Schistosomiasis mansoni / Q000473:pathology / Q000503:physiopathology*; D012701:Serotonin / Q000494:pharmacology; D013270:Stomach / Q000187:drug effects / Q000473:pathology / Q000503:physiopathology*; D013997:Time Factors
D015415:Biomarkers; D007375:Interleukin-1; D012701:Serotonin; D011189:Potassium Chloride; D015237:Dinoprost; D009195:Peroxidase