Immunohistochemical detection of cytokeratin 18 and its neo-epitope in Warthin's tumor (adenolymphoma) of the parotid glands.
An immunohistochemical method using a monoclonal antibody M30 (MAb M30), which reacts with the product released by cleavage of cytokeratin 18 (CK18) by activated caspase, was used to investigate the presence and extent of apoptosis in 36 cases of Warthin's tumor (WT) of the parotid glands. The distribution of CK18 in WT was also determined and compared with that of the product detected by MAb M30. In WT, CK18 was observed mainly in the tumor cells of duct-like structures, but not in the cells of lymphatic tissues. Positive MAb M30 reaction products were found in luminal contents, duct-like structures and the cytoplasm of some macrophages in lymphatic areas near the duct-like structures in WT. These findings indicated that apoptotic cells are phagocytosed and eliminated as waste by macrophages. It is suggested that a mechanism which regulates the balance of proliferative activity and apoptosis may be closely linked to the growth of WT.
Fukuda
M
M
Second Department of Oral and Maxillofacial Surgery, Meikai University School of Dentistry, 1-1 Keyakidai, Sakado, Saitama 350-0283, Japan. fukudam@dent.meikai.ac.jp
Tanaka
A
A
Kitada
M
M
Fukuda
F
F
Suzuki
S
S
Jiang
Y
Y
Kebusa
Y
Y
Ohno
J
J
Yamamoto
Y
Y
Minato
H
H
Nonomura
A
A
Sakashita
H
H
Kusama
K
K
D000235:Adenolymphoma / Q000737:chemistry* / Q000473:pathology*; D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal / Q000276:immunology; D017209:Apoptosis; D000939:Epitopes / Q000032:analysis / Q000276:immunology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007633:Keratins / Q000032:analysis* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D010306:Parotid Gland / Q000737:chemistry* / Q000473:pathology
D000911:Antibodies, Monoclonal; D000939:Epitopes; D007633:Keratins