title: Characterization of the mutS-proximal region of the Salmonella typhimurium SPI-1 identifies a group of pathogenicity island-associated genes.
abstract: The virulence properties of Salmonella enterica are largely encoded within a set of horizontally acquired gene blocks termed pathogenicity islands. One such pathogenicity island, SPI-1, located at centisome 63 of the Salmonella chromosome between the mutS and fhlA genes, encodes a type III protein secretion system and an iron uptake system. We have characterized the mutS-proximal border of this pathogenicity island and have identified two sets of genes, pigAB and pigCD. All four genes have homologs of unknown function in several bacteria that share the ability to establish an intimate association with higher eukaryotic hosts. The expression of at least two of these genes, pigA and pigB, is controlled by SprA, a transcription factor encoded within SPI-1 that controls the expression of genes associated with the type III secretion system of this island. In addition, we found that homologs of the pig genes are also found at different locations of the S. enterica chromosome in association with segments of DNA that exhibit features of pathogenicity islands. The presence of several apparently functional copies of these genes argues for an important role in the biology of this bacterial pathogen. Furthermore, they constitute a valuable tool to identify potential pathogenicity islands.
authors: Section of Microbial Pathogenesis, Boyer Center for Molecular Medicine, Yale School of Medicine, New Haven, CT 06536-0812, USA.
authors: A
authors: A
authors: Pancetti
authors: J E
authors: JE
authors: Galán
mesh_terms: D000818:Animals; D002460:Cell Line; D002874:Chromosome Mapping; D005798:Genes, Bacterial; D005838:Genotype; D051379:Mice; D008807:Mice, Inbred BALB C; D009154:Mutation; D012486:Salmonella typhimurium / Q000235:genetics* / Q000472:pathogenicity; D014774:Virulence / Q000235:genetics