Preparative isolation and purification of acteoside and 2'-acetyl acteoside from Cistanches salsa (C.A. Mey.) G. Beck by high-speed counter-current chromatography.
High-speed counter-current chromatography (HSCCC) was applied to the separation and purification of phenylethanoid glycosides (PhGs) acteoside and 2'-acetylacteoside from Cistanches salsa (C.A. Mey) G. Beck with a quaternary two-phase solvent system composed of ethyl acetate-n-butanol-ethanol-water (4:0.6:0.6:5, v/v). HPLC analyses of the CCC fractions revealed that the two main PhGs were over 98% purity. Their chemical structures were identified by 1H NMR, 13C NMR and MS.
Lei
L
L
School of Pharmaceutical Sciences, Peking University, Beijing, China.
Yang
F
F
Zhang
T
T
Tu
P
P
Wu
L
L
Ito
Y
Y
D002851:Chromatography, High Pressure Liquid; D003377:Countercurrent Distribution / Q000379:methods*; D005960:Glucosides / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy; D019684:Magnoliopsida / Q000737:chemistry*; D010636:Phenols; D059808:Polyphenols
D005960:Glucosides; D010636:Phenols; C111522:2'-acetylacteoside; C058956:acteoside; D059808:Polyphenols