Lack of effect of the abnormal fatty acid metabolism in NC/Nga mice on their atopic dermatitis.
Although clinical evidence has suggested that dysregulated fatty acid metabolism is associated with atopic disorders, the molecular basis for such a correlation remains to be demonstrated. In the present study, we analyzed the fatty acid composition in peripheral blood cells of NC/Nga mice, a model for atopic dermatitis (AD). We found that arachidonic acid significantly accumulated in mice with the AD manifestation. In addition, the leucotriene B4-releasing ability upon calcium ionophore A23187 stimulation was potentiated in blood cells. An arachidonic acid accumulation was not apparent in the non-atopic BALB/c strain, but was still observed in healthy NC/Nga mice fed under specific pathogen-free conditions. These results indicate that a disturbed fatty acid metabolism in NC/Nga mice was not a trigger factor for their dermatitis development.
Kawamoto
S
S
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Higashi-Hiroshima, Japan. skawa@hiroshima-u.ac.jp
Kita
M
M
Hamada
M
M
Aki
T
T
Shigeta
S
S
Suzuki
O
O
Ono
K
K
D000818:Animals; D016718:Arachidonic Acid / Q000097:blood; D001773:Blood Cells / Q000187:drug effects / Q000378:metabolism; D000001:Calcimycin / Q000494:pharmacology; D003876:Dermatitis, Atopic / Q000097:blood* / Q000235:genetics; D004195:Disease Models, Animal; D005227:Fatty Acids / Q000097:blood* / Q000737:chemistry; D006801:Humans; D066298:In Vitro Techniques; D007476:Ionophores / Q000494:pharmacology; D007975:Leukotriene B4 / Q000097:blood; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008817:Mice, Mutant Strains; D017962:alpha-Linolenic Acid / Q000097:blood
D005227:Fatty Acids; D007476:Ionophores; D017962:alpha-Linolenic Acid; D007975:Leukotriene B4; D016718:Arachidonic Acid; D000001:Calcimycin