title: Mucosal immune response of spotted sand bass Paralabrax maculatofasciatus (Steindachner, 1868) orally immunised with an extracellular lectin of Aeromonas veronii.
abstract: To assess the immunogenic and immunoprotective role of the extracellular lectin from Aeromonas veronii (MCBP), which has affinity for mucosal constituents such as mucin, lactoferrin, immunoglobulins and collagen, spotted sand bass (Paralabrax maculatofasciatus) were orally immunised either with soluble MCBP, adjuvant-conjugated MCBP or immobilised MCBP on latex microspheres. The results suggest that the MCBP is capable of eliciting protective immunity against A. veronii infections when administered orally. The highest mucosal immune response was elicited in fish immunised with MCBP covalently linked to cholera toxin B subunit (CTB) or to Escherichia coli heat-labile toxin (hLT). MCBP-CTB was found to elicit immunoprotection against a challenge with live Aeromonas cells with a relative percent survival of almost 70% and without the expression of the severe histopathological alterations induced by A. veronii.
authors: Department of Marine Pathology, Center for Biological Research, Baja California Sur, Mexico.
authors: M L
authors: ML
authors: Merino-Contreras
authors: M A
authors: MA
authors: Guzman-Murillo
authors: E
authors: E
authors: Ruiz-Bustos
authors: M J
authors: MJ
authors: Romero
authors: M A
authors: MA
authors: Cadena-Roa
authors: F
authors: F
authors: Ascencio
mesh_terms: D000333:Aeromonas / Q000737:chemistry / Q000276:immunology*; D000818:Animals; D001427:Bacterial Toxins / Q000276:immunology; D001492:Bass / Q000276:immunology*; D002772:Cholera Toxin / Q000276:immunology; D004768:Enterotoxins / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D029968:Escherichia coli Proteins; D005393:Fish Diseases / Q000517:prevention & control*; D016905:Gram-Negative Bacterial Infections / Q000517:prevention & control / Q000662:veterinary*; D018928:Immunity, Mucosal; D037102:Lectins / Q000276:immunology*; D014611:Vaccination / Q000662:veterinary
chemical_list: D001427:Bacterial Toxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D037102:Lectins; D002772:Cholera Toxin; C027538:heat-labile enterotoxin, E coli