Cloning and expression of a novel cysteine-rich secreted protein family member expressed in thyroid and pancreatic mesoderm within the chicken embryo.
We have isolated a new chicken gene that is a member of the cysteine-rich secreted protein family (CRISP). The CRISP family is composed of over 70 members that are found in many phyla of organisms, including: vertebrates, plants, fungi, yeast, and insects. Here we describe the cloning of a novel member of this family, SugarCrisp, and its expression pattern throughout chicken embryogenesis. We also describe its utility as a marker of thyroid and pancreatic mesoderm in the developing chicken embryo and its expression within the human and mouse in glandular tissue.
Smith
D M
DM
Department of Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA.
Collins-Racie
L A
LA
Marigo
V A
VA
Roberts
D J
DJ
Davis
N M
NM
Hartmann
C
C
Schweitzer
R
R
LaVallie
E R
ER
Gamer
L
L
McCoy
J
J
Tabin
C J
CJ
D000595:Amino Acid Sequence; D000818:Animals; D030161:Avian Proteins; D002642:Chick Embryo; D003001:Cloning, Molecular; D003545:Cysteine / Q000737:chemistry*; D018076:DNA, Complementary / Q000378:metabolism; D004625:Embryo, Nonmammalian / Q000378:metabolism; D006801:Humans; D017403:In Situ Hybridization; D008168:Lung / Q000196:embryology; D008648:Mesoderm / Q000378:metabolism*; D008969:Molecular Sequence Data; D010179:Pancreas / Q000196:embryology*; D014176:Protein Biosynthesis; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013961:Thyroid Gland / Q000196:embryology*; D014018:Tissue Distribution
D030161:Avian Proteins; D018076:DNA, Complementary; D011506:Proteins; C432428:SugarCrisp protein, chicken; D003545:Cysteine