Correlations between two markers of sperm DNA integrity, DNA denaturation and DNA fragmentation, in fertile and infertile men.
OBJECTIVE
To evaluate two different assays of human sperm DNA integrity, DNA denaturation (DD) and DNA fragmentation (DF), and to correlate these with standard semen parameters.


METHODS
Prospective, observational study.


METHODS
University infertility clinic.


METHODS
Forty consecutive semen samples from 33 nonazoospermic men presenting for infertility evaluation and 7 fertile men presenting for vasectomy.


METHODS
Assessment of sperm concentration, motility, morphology, DD and DF.


METHODS
Sperm DD and DF in fertile and infertile men.


RESULTS
The mean (+/-SE) rates of DD and DF were significantly higher in infertile subjects compared to fertile controls, respectively: 25.4 +/- 3.0 vs. 10.2 +/- 2.3 (P=.028) and 27.6 +/- 2.5 vs. 13.3 +/- 2.5% (P=.016). DF and DD correlated strongly (r = 0.71, P<.0001). Also, DD and DF correlated negatively with standard semen parameters (concentration, motility, and morphology), the strongest correlation being with sperm motility.


CONCLUSIONS
The strong correlation between sperm DD and DF, and the higher levels of sperm DNA damage in infertile compared with fertile men, indicate that male infertility is associated with poor sperm DNA integrity. Although infertile men may father children with assisted conception, fertilization with DNA-damaged spermatozoa may increase the risk of genetic disease in the offspring.
Zini
A
A
Division of Urology, Department of Surgery, University of Toronto, Ontario, Toronto, Canada. azini@mtsinai.on.ca
Bielecki
R
R
Phang
D
D
Zenzes
M T
MT
D015415:Biomarkers / Q000032:analysis; D004247:DNA / Q000032:analysis*; D053938:DNA Fragmentation; D005298:Fertility / Q000235:genetics / Q000502:physiology*; D006801:Humans; D020287:In Situ Nick-End Labeling; D007248:Infertility, Male / Q000235:genetics / Q000503:physiopathology*; D008297:Male; D009691:Nucleic Acid Denaturation; D012016:Reference Values; D012661:Semen; D013076:Sperm Count; D013081:Sperm Motility; D013094:Spermatozoa / Q000502:physiology*; D014659:Vasectomy
D015415:Biomarkers; D004247:DNA