Myristoylation of human immunodeficiency virus type 1 gag protein is required for efficient env protein transportation to the surface of cells.
Highly conserved amino acids in the N-terminal region of the human immunodeficiency virus type 1 (HIV-1) Pr55(gag) are recognized to be critical for the attachment of myristic acid. We previously reported that the env protein was not detected on the cell surface by blocking of N-myristoylation of Pr55(gag) with N-myristoyl glycinal diethylacetal. Here, we constructed a mutant by substituting the N-terminal glycine of Pr55(gag) with alanine to demonstrate that N-myristoylation of Pr55(gag) is required for efficient env protein transportation to the cell surface. The expression level of the env protein on the surface of Jurkat cells transfected with the myristoylation-defective phenotype was observed to be significantly reduced by electron microscopic analyses with a gold-labeled monoclonal antibody against the env protein. In addition, Jurkat cells transfected with the myristoylation-defective phenotype lost the ability of envelope-mediated cell-to-cell fusion. The results suggest that N-myristoylation of the HIV-1 gag protein is necessary for efficient env protein transportation to the cell surface.
Shiraishi
T
T
Department of Biochemistry, Kumamoto University, Kumamoto, 862-0973, Japan.
Misumi
S
S
Takama
M
M
Takahashi
I
I
Shoji
S
S
D019943:Amino Acid Substitution; D015153:Blotting, Western; D002458:Cell Fractionation; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure; D015683:Gene Products, gag / Q000235:genetics / Q000378:metabolism*; D015726:Giant Cells / Q000821:virology; D015700:HIV Envelope Protein gp41 / Q000378:metabolism*; D006801:Humans; D019169:Jurkat Cells / Q000378:metabolism / Q000648:ultrastructure; D008561:Membrane Fusion / Q000502:physiology; D016297:Mutagenesis, Site-Directed; D019814:Myristic Acid / Q000378:metabolism*; D011498:Protein Precursors / Q000235:genetics / Q000378:metabolism*; D021381:Protein Transport / Q000502:physiology; D013601:T-Lymphocytes / Q000737:chemistry / Q000166:cytology / Q000821:virology; D014162:Transfection
D015683:Gene Products, gag; D015700:HIV Envelope Protein gp41; D011498:Protein Precursors; C059767:p55 gag precursor protein, Human immunodeficiency virus 1; D019814:Myristic Acid