[Activity of transmembrane calcium transport and levels of endothelin-1 in patients with variant angina].
Recent studies showed that coronary artery spasm may be due to disturbances of secretory and excretory endothelial activity in atherosclerotic coronary artery. However, this theory does not explain the reasons of coronary artery spasm when endothelium is not damaged. There must be other patomechanisms of coronary artery spasm. The aim of our study was examination of calcium efflux through the lymphocytic cell membrane and determination of endothelin-1 plasma levels in patients with variant angina in order to define the participation of these factors in pathogenesis of coronary artery spasm. The survey was made in 76 patients with ischaemic heart disease. All patients were divided into 2 groups. The first group consisted of 48 patients with variant angina (d.b.s.), the other consisted of 28 patients with stable angina (d.b.w.). The control group (g.k.) was composed of 25 healthy people. Patients were administered 100 ml of trometamol (TRIS, pH = 10.5) intravenously for 5 minutes. After stopping the infusion the examined patient was breathing deeply for 5 minutes at a rate of 40/min. The endothelin-1 (ET-1) plasma levels and transmembrane calcium transport in lymphocytes were determined before and just after the hyperventilation test, as well as 10 minutes after the test. ET-1 plasma concentrations were estimated with a radioimmunologic assay. The method of estimation of transmembrane calcium transport was elaborated in Laboratory of Department of Cardiology of Medical University of Wrocław. We showed that ET-1 plasma levels and transmembrane calcium transport in patients with d.b.s. before the test were normal. There was an increase in transmembrane calcium efflux in patients with d.b.s. during coronary artery spasm that had been caused by ET-1. ET-1 plasma levels were still high 10 min. after the coronary artery spasm. Disturbances of transmembrane calcium transport and increased endothelin-1 plasma level may be the primary factors responsible for coronary artery spasm.
Halawa
B
B
Katedra i Klinika Kardiologii Akademii Medycznej we Wrocławiu.
Salomon
P
P
D000328:Adult; D000368:Aged; D000787:Angina Pectoris / Q000378:metabolism; D000788:Angina Pectoris, Variant / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D019332:Endothelin-1 / Q000097:blood*; D005260:Female; D006801:Humans; D017136:Ion Transport; D008214:Lymphocytes / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D014325:Tromethamine
D019332:Endothelin-1; D014325:Tromethamine; D002118:Calcium