title: Tissue-engineered product: allogeneic cultured dermal substitute composed of spongy collagen with fibroblasts.
abstract: Recently, various types of allogeneic skin substitutes including cultured epidermal substitute (CES), cultured dermal substitute (CDS), and cultured skin substitute (CSS), which are composed of keratinocytes and/or fibroblasts as the cellular component(s), have been used as biological wound dressings. In our study, the allogeneic CDS was prepared by plating fibroblasts on a spongy collagen. The clinical evaluation was conducted using fresh or cryopreserved allogeneic CDS. In 145 of our clinical cases, 95% (138/145) of various wounds were evaluated as achieving good or excellent results, including 96% (22/23) of deep dermal burns (DDB) and dermal burns (DB), 100% (53/53) of partial-thickness donor wounds, 91% (21/23) of traumatic skin defects, 100% (5/5) of pressure ulcers, 82% (9/11) of chronic skin ulcers, 100% (6/6) of coverage for debrided DB, and 92% (22/24) of coverage for autologous meshed graft. The results obtained in our study suggest that the allogeneic CDS is able to provide an effective therapy for patients with partial and/or full-thickness skin defects.
authors: R & D Center for Artificial Skin, School of Allied Health Sciences, Kitasato University, Sagamihara, Japan.
authors: Y
authors: Y
authors: Kuroyanagi
authors: N
authors: N
authors: Yamada
authors: R
authors: R
authors: Yamashita
authors: E
authors: E
authors: Uchinuma
mesh_terms: D000368:Aged; D001698:Biomedical Engineering; D002056:Burns / Q000628:therapy; D002478:Cells, Cultured; D003094:Collagen; D020405:Dermis / Q000166:cytology*; D005347:Fibroblasts / Q000166:cytology*; D006801:Humans; D008297:Male; D012883:Skin Ulcer / Q000628:therapy; D017768:Skin, Artificial; D014945:Wound Healing
chemical_list: D003094:Collagen