Enhanced throughput for DNA sequencing by capillary array electrophoresis with a gradient of electric field strength.
The effect of electric field gradients are examined on the speed, selectivity, read length, and accuracy for DNA sequencing using capillary array electrophoresis. Modified electric field gradients was realized to read over 800 bases within 140 min. The method developed is effectively applicable to single nucleotide polymorphism analysis for genomic drug discovery and pharmacogenomics.
Yoshida
C
C
Department of Medicinal Chemistry, Faculty of Pharmaceutical Sciences, The University of Tokushima, Shomachi, 770-8505, Tokushima, Japan.
Endo
Y
Y
Baba
Y
Y
D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D017422:Sequence Analysis, DNA / Q000295:instrumentation / Q000379:methods*