title: Partial resistance of low density lipoprotein to oxidation in vivo after increased intake of berries.
abstract: OBJECTIVE
  The health-promoting effects of fruit- and vegetable-based diets are known to be associated with their antioxidative components. We found in our preliminary in vitro laboratory tests that extracts of many common Finnish edible berries are potent scavengers of peroxyl radicals and inhibitors of lipid peroxidation. We therefore designed the current study to evaluate both the long-term (8 weeks) and short-term (5 hours) effects of increased intake of three berries on antioxidant potential and lipid peroxidation.
  
  
  RESULTS
  Healthy 60-year-old men were randomized to berry, supplement and control groups (20 men in each group). The berry group ate, in addition to their normal diet, a 100 g portion of deep-frozen berries (bilberries, lingonberries, or black currants) daily for 8 weeks. The other groups ingested daily 100 mg of alpha-tocopherol and 500 mg of ascorbic acid (supplement group) or 500 mg of calcium gluconate (control group). In the short-term experiment 6 men ate 80 g of each of the three berries in one go. Serum ascorbate concentrations increased significantly in both the berry and the supplement group. Serum alpha-tocopherol levels and the antioxidant potential (TRAP) in low density lipoprotein (LDL) increased in the supplement group only. In the berry group, slightly lowered LDL diene conjugation (p = 0.074) and slightly increased total serum TRAP (p = 0.084) values were observed. No changes were found in these measures in the supplement or the control group. In the short-term experiment, LDL TRAP showed a small increase (about 10%, p = 0.039) during five hours after the intake of 240 g berries.
  
  
  CONCLUSIONS
  The effects of consumption of berries on antioxidant potential and diene conjugation in LDL particles in vivo appear to be small.
authors: Research and Development Centre, Social Insurance Institution, University of Turku, Finland.
authors: J
authors: J
authors: Marniemi
authors: P
authors: P
authors: Hakala
authors: J
authors: J
authors: Mäki
authors: M
authors: M
authors: Ahotupa
mesh_terms: D000975:Antioxidants / Q000378:metabolism; D001205:Ascorbic Acid / Q000097:blood*; D015331:Cohort Studies; D019587:Dietary Supplements; D005638:Fruit / Q000627:therapeutic use*; D006801:Humans; D015227:Lipid Peroxidation; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D010084:Oxidation-Reduction; D008517:Phytotherapy; D014810:Vitamin E / Q000097:blood*
chemical_list: D000975:Antioxidants; D008077:Lipoproteins, LDL; D014810:Vitamin E; D001205:Ascorbic Acid