Characterization of intragenic recombination in a hyperthermophilic archaeon via conjugational DNA exchange.
Sulfolobus acidocaldarius is so far the only hyperthermophilic archaeon in which genetic recombination can be assayed by conjugation and simple selections. Crosses among spontaneous pyr mutants were able to resolve closely spaced chromosomal mutations, identify deletions and rearrangements, and map mutations to a given deletion interval. Frameshift mutations in pyrE exerted polar effects that depressed orotidine-5'-monophosphate decarboxylase activity (encoded by pyrF), whereas base pair substitutions and an 18-bp deletion had no effect.
Reilly
M S
MS
Department of Biological Sciences, University of Cincinnati, Ohio 54221-0006, USA.
Grogan
D W
DW
D000483:Alleles; D019843:Archaeal Proteins; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D003227:Conjugation, Genetic; D004268:DNA-Binding Proteins; D004352:Drug Resistance, Microbial; D016368:Frameshift Mutation; D019766:Genes, Archaeal; D034982:Integration Host Factors; D009963:Orotic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D011401:Promoter Regions, Genetic; D011549:Pseudomonas; D011995:Recombination, Genetic; D016945:Sulfolobus acidocaldarius / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D013696:Temperature; D015534:Trans-Activators / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
D019843:Archaeal Proteins; D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C080133:DmpR protein, Pseudomonas; D034982:Integration Host Factors; D015534:Trans-Activators; D014157:Transcription Factors; C440503:integration host factor, Pseudomonas; C407406:pyrF protein, Archaeal; D009963:Orotic Acid; C001242:5-fluoroorotic acid