Activin type II receptors in embryonic dorsal root ganglion neurons of the chicken.
Activin induces neuropeptide expression in chicken ciliary ganglion neurons. To determine if activin might also influence neuropeptide expression in developing sensory neurons, we examined whether type II activin receptors are expressed during embryonic development of the chicken dorsal root ganglia (DRG), and also examined the effects of activin on neuropeptide expression in cultured DRG neurons. Using reverse transcription polymerase chain reaction (rtPCR), we detected mRNAs for both the activin receptors type IIA (ActRIIA) and type IIB (ActRIIB) in DRG from embryonic day 7 through posthatch day 1. With in situ hybridization, we found that morphologically identifiable neurons express mRNAs for both ActRIIA and ActRIIB. With developmental age, a subset of neurons that hybridizes more intensely with riboprobes to these receptor mRNAs becomes evident. A similar pattern of expression is observed with immunocytochemical staining using antisera against activin type II receptors. To examine whether embryonic DRG cells respond to activin we treated dissociated cultures of DRG with activin A and assessed the expression of vasoactive intestinal peptide (VIP) and calcitonin gene related peptide (CGRP) mRNAs using semiquantitative rtPCR. Activin treatment results in an increase in VIP mRNA, but does not affect CGRP mRNA levels. These observations indicate that neurons in the embryonic chicken DRG can respond to activin and suggest that activin has the potential to play a role in the development and function of DRG sensory neurons.
Kos
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Department of Anatomy and Cell Biology, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, Maryland 20814, USA.
Fine
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Coulombe
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D030301:Activin Receptors, Type II; D000818:Animals; D000906:Antibodies; D015740:Calcitonin Gene-Related Peptide / Q000235:genetics; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D002642:Chick Embryo; D002645:Chickens; D005727:Ganglia, Spinal / Q000737:chemistry / Q000166:cytology* / Q000196:embryology*; D018507:Gene Expression Regulation, Developmental; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D009474:Neurons / Q000737:chemistry / Q000166:cytology / Q000502:physiology*; D012333:RNA, Messenger / Q000032:analysis; D017978:Receptors, Growth Factor / Q000032:analysis / Q000235:genetics* / Q000276:immunology; D014660:Vasoactive Intestinal Peptide / Q000235:genetics
D000906:Antibodies; D012333:RNA, Messenger; D017978:Receptors, Growth Factor; D014660:Vasoactive Intestinal Peptide; D030301:Activin Receptors, Type II; D015740:Calcitonin Gene-Related Peptide