Intestinal cell proliferation is influenced by intakes of protein and energy, aflatoxin, and whole-body radiation.
Intestinal epithelial cell proliferation in young male F344 rats was measured in response to dietary protein content (5%, 10%, and 20% casein diets), energy restriction (energy intake was 60% of ad libitum energy intakes of animals consuming the 20% casein diet), total diet restriction (dietary intake was 60% of the ad libitum intake of 20% casein diet group), aflatoxin administration, and whole body irradiation. Cellular proliferation was measured in sections of jejunum, ileum, proximal colon, and distal colon with the [3H]thymidine technique. Restricting energy or total diet intakes by 40% from ad libitum levels reduced proliferation in epithelial cells throughout the intestine. In comparison to the 5% casein diet, the 20% casein diet resulted in modestly lower cellular proliferation in all intestinal segments. Radiation induced a decrease in cellular proliferation in the jejunum and ileum; this decrease was prevented by a 20% casein diet. Pretreatment with aflatoxin B1 decreased intestinal cell proliferation throughout the intestine, and this decrease was not influenced by the protein content of the diet.
Fleming
S E
SE
Department of Nutritional Sciences, University of California, Berkeley, CA 94720, USA.
Youngman
L D
LD
Ames
B N
BN
D000348:Aflatoxins / Q000008:administration & dosage*; D000818:Animals; D002364:Caseins / Q000008:administration & dosage; D002455:Cell Division / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D003106:Colon; D004044:Dietary Proteins / Q000008:administration & dosage*; D002149:Energy Intake; D007082:Ileum; D007413:Intestinal Mucosa / Q000166:cytology* / Q000187:drug effects / Q000528:radiation effects; D007583:Jejunum; D008297:Male; D051381:Rats; D011916:Rats, Inbred F344; D015430:Weight Gain; D014916:Whole-Body Irradiation
D000348:Aflatoxins; D002364:Caseins; D004044:Dietary Proteins