Control of a genetic regulatory network by a selector gene.
The formation of many complex structures is controlled by a special class of transcription factors encoded by selector genes. It is shown that SCALLOPED, the DNA binding component of the selector protein complex for the Drosophila wing field, binds to and directly regulates the cis-regulatory elements of many individual target genes within the genetic regulatory network controlling wing development. Furthermore, combinations of binding sites for SCALLOPED and transcriptional effectors of signaling pathways are necessary and sufficient to specify wing-specific responses to different signaling pathways. The obligate integration of selector and signaling protein inputs on cis-regulatory DNA may be a general mechanism by which selector proteins control extensive genetic regulatory networks during development.
Guss
K A
KA
Howard Hughes Medical Institute and Laboratory of Molecular Biology, University of Wisconsin, Madison, WI 53706, USA.
Nelson
C E
CE
Hudson
A
A
Kraus
M E
ME
Carroll
S B
SB
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D004247:DNA / Q000235:genetics / Q000378:metabolism; D018983:DNA Footprinting; D004268:DNA-Binding Proteins / Q000378:metabolism; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000196:embryology* / Q000235:genetics* / Q000254:growth & development; D018507:Gene Expression Regulation, Developmental; D017344:Genes, Insect / Q000235:genetics*; D017930:Genes, Reporter / Q000235:genetics; D007814:Larva / Q000254:growth & development / Q000378:metabolism; D008957:Models, Genetic; D009154:Mutation / Q000235:genetics; D009928:Organ Specificity; D020218:Response Elements / Q000235:genetics; D015398:Signal Transduction; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014921:Wings, Animal / Q000196:embryology / Q000378:metabolism
D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D014157:Transcription Factors; C087984:sd protein, Drosophila; D004247:DNA