title: Role of amniotic fluid interphase fluorescence in situ hybridization (FISH) analysis in patient management.
abstract: We retrospectively reviewed 309 amniotic fluid interphase fluorescence in situ hybridization (FISH) analyses performed from October 1995 to June 1999 to assess the role of interphase FISH in the management of patients at increased risk for fetal aneuploidies. Gestational age and indications for amniocentesis, clinical interventions after FISH results, as well as interventions after final culture reports were analyzed. There were 244 (79%) normal, 50 (16%) abnormal and 15 (5%) inconclusive FISH results. There were no false-positive or false-negative results, but there were nine (3%) clinically significant chromosomal abnormalities not detectable by FISH. Of the 50 women with abnormal FISH results, 26 (52%) elected to terminate the pregnancy prior to the availability of the standard chromosome analysis. In two of the fetuses with trisomy 21 no abnormalities were reported by ultrasound examination. Our experience indicates that interphase FISH results played an important role in decision making, especially for pregnancies close to 24 weeks' gestation. Standard karyotype analysis is still required for detection of chromosome abnormalities not detectable by interphase FISH techniques and for clarification of unusual or inconclusive FISH results.
authors: Fetal Assessment & Treatment Unit, Department of Obstetrics & Gynaecology, Mount Sinai Hospital, University of Toronto, Toronto, Canada.
authors: W
authors: W
authors: Cheong Leung
authors: D
authors: D
authors: Chitayat
authors: G
authors: G
authors: Seaward
authors: R
authors: R
authors: Windrim
authors: G
authors: G
authors: Ryan
authors: J
authors: J
authors: Barrett
authors: E J
authors: EJ
authors: Winsor
mesh_terms: D000653:Amniotic Fluid / Q000166:cytology*; D000782:Aneuploidy; D002869:Chromosome Aberrations; D005260:Female; D005865:Gestational Age; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007399:Interphase; D007621:Karyotyping; D011247:Pregnancy; D012189:Retrospective Studies; D016216:Ultrasonography, Prenatal