title: Creatine kinase isoforms in the seminal plasma and the purified human sperm.
abstract: In previous conventional reports sperm and seminal plasma were not well separated and therefore it was highly probable that isoform (CK-BB) of seminal plasma was determined together with that of sperm. The authors developed a method to purify sperm specimen free from seminal plasma. Human sperm was purified by means of the continuous-step density gradient centrifugation with an inner column and the subsequent modified swim-up procedure. CK isoforms in the purified sperm and the seminal plasma were analyzed by ion exchange chromatography and electrophoresis. The purified sperm contained mitochondrial CK-MiMi as well as cytosolic CK, of which isoform was almost occupied by CK-MM but CK-BB was not detected. On the other hand, CK isoform in the seminal plasma was detected as CK-BB alone. CK activity in human sperm and seminal plasma were 2.0 +/- 0.7 x 10(-6) IU/10(6) sperm and 661 +/- 360 IU/L, respectively. CK activity in the seminal plasma gave no correlation with the semen parameters.
authors: Department of Urology, Ichikawa General Hospital, Tokyo Dental College, Chiba, Japan. miyazi@tdc.ac.jp
authors: K
authors: K
authors: Miyaji
authors: S
authors: S
authors: Kaneko
authors: H
authors: H
authors: Ishikawa
authors: T
authors: T
authors: Aoyagi
authors: K
authors: K
authors: Hayakawa
authors: M
authors: M
authors: Hata
authors: M
authors: M
authors: Oohashi
authors: A
authors: A
authors: Izawa
authors: M
authors: M
authors: Murai
mesh_terms: D002469:Cell Separation; D002499:Centrifugation, Density Gradient; D002852:Chromatography, Ion Exchange; D003402:Creatine Kinase / Q000032:analysis / Q000378:metabolism*; D004588:Electrophoresis, Cellulose Acetate; D006801:Humans; D007527:Isoenzymes; D008297:Male; D012661:Semen / Q000201:enzymology*; D013081:Sperm Motility; D013094:Spermatozoa / Q000201:enzymology*
chemical_list: D007527:Isoenzymes; D003402:Creatine Kinase