Specificity of lectin activity of Bacillus thuringiensis parasporal inclusion proteins.
Sheep erythrocyte-agglutinating parasporal inclusion proteins from four Bacillus thuringiensis strains (FITC-20, FITC-73, FITC-76 and IBC-1456) were examined for lectin activity against erythrocytes from four mammalian (rabbit, horse, cow and guinea pig) and an avian (chicken) species. Of the five erythrocyte species, only rabbit cells were agglutinated with the protein of the human cancer cell-killing strain IBC-1456. No haemagglutination (HA) activities were shown in other protein-erythrocyte combinations. The lectin activity of the strain IBC-1456 against rabbit cells was strongly inhibited by preincubation with D-galactose. Overall results revealed that the B. thuringiensis lectins have a preference for sheep erythrocytes.
Akao
T
T
Biotechnology & Food Research Institute, Fukuoka Industrial Technology Centre, Kurume, Fukuoka, Japan.
Mizuki
E
E
Yamashita
S
S
Kim
H S
HS
Lee
D W
DW
Ohba
M
M
D000818:Animals; D001413:Bacillus thuringiensis; D001426:Bacterial Proteins / Q000302:isolation & purification* / Q000494:pharmacology; D002417:Cattle; D002645:Chickens; D006168:Guinea Pigs; D006388:Hemagglutinins / Q000302:isolation & purification* / Q000494:pharmacology; D006736:Horses; D037102:Lectins / Q000302:isolation & purification* / Q000494:pharmacology; D011817:Rabbits; D013045:Species Specificity; D013171:Spores, Bacterial
D001426:Bacterial Proteins; D006388:Hemagglutinins; D037102:Lectins