Adult bone marrow transplantation after stroke in adult rats.
We transplanted adult whole bone marrow prelabeled with bromodeoxyuridine (BrdU) into the ischemic boundary zone of the adult rat brain at 1 day after 2 h of middle cerebral artery occlusion (MCAo). Approximately 3.3% of 10(6) transplanted bone marrow cells were BrdU reactive at 14 days after MCAo. BrdU-reactive cells expressed neuronal and astrocytic proteins, neuronal nuclei protein (NeuN, 1%), and glial fibrillary acidic protein (GFAP, 5%) immunoreactivities, respectively. In addition, bone marrow transplantation promoted proliferation of ependymal and subependymal cells, identified by nestin (a neuroepithelial stem cell marker), within the ventricular zone and subventricular zone (VZ/SVZ). These data suggest that intracerebral transplantation of bone marrow could potentially be used to induce plasticity in ischemic brain.
Li
Y
Y
Department of Neurology, Henry Ford Health Sciences Center, Detroit, MI 48202, USA.
Chen
J
J
Chopp
M
M
D000818:Animals; D016026:Bone Marrow Transplantation / Q000473:pathology / Q000502:physiology; D001973:Bromodeoxyuridine / Q000378:metabolism; D002454:Cell Differentiation; D002465:Cell Movement; D002470:Cell Survival; D007150:Immunohistochemistry; D007381:Intermediate Filament Proteins / Q000378:metabolism; D008297:Male; D009419:Nerve Tissue Proteins; D064231:Nestin; D010641:Phenotype; D051381:Rats; D017208:Rats, Wistar; D020521:Stroke / Q000378:metabolism / Q000473:pathology / Q000628:therapy*
D007381:Intermediate Filament Proteins; D009419:Nerve Tissue Proteins; C577486:Nes protein, rat; D064231:Nestin; D001973:Bromodeoxyuridine