Reciprocal down-regulation of p53 and SOD2 gene expression-implication in p53 mediated apoptosis.
p53 regulates the transcription of a number of genes among which are different redox-related genes. It has been proposed that these genes can induce a cellular oxidative stress leading to p53-dependent apoptosis (Polyak et al., 1997). MnSOD, the product of superoxide dismutase 2 (SOD2) gene, is one of the major cellular defences against oxidative stress. We demonstrate here that p53 is able to repress SOD2 gene expression and that this repression takes place at promoter level. We show the importance of this regulation for the p53 function, by demonstrating that an overexpression of MnSOD decreases p53-mediated induction of apoptosis. Moreover, we demonstrate that MnSOD overexpression decreases p53-gene expression at the promoter level. These findings raise the hypothesis that p53 and SOD2 genes are mutually regulated leading to the modulation of various cellular processes including apoptosis.
Drane
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P
Commissariat à l'Energie Atomique (CEA), Laboratoire de Cancérogenèse Moléculaire, UMR217 CEA-CNRS, DRR, DSV, BP6 92265 Fontenay-aux-Roses Cedex, France.
Bravard
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A
Bouvard
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V
May
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E
D017209:Apoptosis / Q000235:genetics*; D001943:Breast Neoplasms; D015536:Down-Regulation; D005260:Female; D006801:Humans; D016328:NF-kappa B / Q000378:metabolism; D018384:Oxidative Stress / Q000235:genetics*; D011401:Promoter Regions, Genetic; D013482:Superoxide Dismutase / Q000235:genetics*; D014407:Tumor Cells, Cultured; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*
D016328:NF-kappa B; D016159:Tumor Suppressor Protein p53; D013482:Superoxide Dismutase