PCR amplification of putative gpa-2 and gpa-3 orthologs from the (A+T)-rich genome of Strongyloides stercoralis.
Two G protein alpha subunit genes orthologous to gpa-2 and gpa-3 in Caenorhabditis elegans have been identified in the parasitic nematode, Strongyloides stercoralis. These genes mediate chemosensory signal transduction regulating dauer arrest in C. elegans. In the parasite, they represent candidate mediators for regulation of the choice between free-living and parasitic life cycles, the obligatory developmental arrest of infective larvae, and reactivation of development after infection. The (A+T) content of these genes is 72.2% for coding sequences, 90% for introns, and 84.1% for 5' and 3' flanking regions, requiring the use of low extension temperatures for long distance PCR. The possible significance of conserved structural motifs of these proteins is discussed.
Massey
H C
HC
Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, 3800 Spruce Street, Philadelphia, PA 19104-6050, USA. hmassey@vet.upenn.edu
Ball
C C
CC
Lok
J B
JB
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D017124:Conserved Sequence; D018509:DNA, Helminth / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D020962:Heterotrimeric GTP-Binding Proteins / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D018510:RNA, Helminth / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D019105:Random Amplified Polymorphic DNA Technique; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D017171:Strongyloides stercoralis / Q000737:chemistry / Q000235:genetics*
D018509:DNA, Helminth; D018510:RNA, Helminth; D020962:Heterotrimeric GTP-Binding Proteins