title: Bovine seminal RNase induces apoptosis in normal proliferating lymphocytes.
abstract: Bovine seminal ribonuclease is a member of the RISBAses (ribonucleases with special biological actions) family. It exerts specific anti-tumor, embryotoxic, aspermatogenic and immunosuppressive activities. The cytotoxic effect of bovine seminal ribonuclease on tumor cells is accompanied by the induction of apoptosis. We provide ultrastructural and flow cytometry evidence of apoptotic death following bovine seminal ribonuclease treatment, in normal cells and phytohemagglutinin-stimulated lymphocytes. Transmission and scanning electron microscopy, which were fully supported by flow cytometry data, showed typical features of apoptosis, such as decreased cell size, chromatin condensation, fragmentation in micronuclei, and the presence of apoptotic bodies.
authors: Università degli Studi di Catania, Dipartimento di Scienze Biomediche, Italy.
authors: F
authors: F
authors: Sinatra
authors: D
authors: D
authors: Callari
authors: M
authors: M
authors: Viola
authors: M T
authors: MT
authors: Longombardo
authors: M
authors: M
authors: Patania
authors: L
authors: L
authors: Litrico
authors: G
authors: G
authors: Emmanuele
authors: E
authors: E
authors: Lanteri
authors: N
authors: N
authors: D'Alessandro
authors: S
authors: S
authors: Travali
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002417:Cattle; D004247:DNA / Q000378:metabolism; D004722:Endoribonucleases / Q000494:pharmacology*; D005434:Flow Cytometry; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects* / Q000502:physiology / Q000648:ultrastructure; D010835:Phytohemagglutinins / Q000494:pharmacology
chemical_list: D000970:Antineoplastic Agents; D010835:Phytohemagglutinins; D004247:DNA; D004722:Endoribonucleases; C032523:ribonuclease SPL