Improvement of FISH mapping resolution on combed DNA molecules by iterative constrained deconvolution: a quantitative study.
Image restoration approaches, such as digital deconvolution, are becoming widely used for improving the quality of microscopic images. However, no quantification of the gain in resolution of fluorescence images is available. We show that, after iterative constrained deconvolution, fluorescent cosmid signals appear to be 25% smaller, and 1.2-kb fragment signals on combed molecules faithfully display the expected length.
Monier
K
K
Laboratoire DyOGen, Unité INSERM U309, Institut Albert Bonniot, La Tronche, France. kmonier@scripps.edu
Heliot
L
L
Rougeulle
C
C
Heard
E
E
Robert-Nicoud
M
M
Vourc'h
C
C
Bensimon
A
A
Usson
Y
Y
D018244:Chromosomes, Artificial, Yeast / Q000235:genetics / Q000378:metabolism; D003360:Cosmids / Q000235:genetics; D015342:DNA Probes / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics / Q000378:metabolism*; D005347:Fibroblasts; D005453:Fluorescence; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D012680:Sensitivity and Specificity; D015003:Yeasts / Q000166:cytology / Q000235:genetics
D015342:DNA Probes; D004271:DNA, Fungal