Identification of barley and rye varieties using matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry with neural networks.
Cereal varieties are normally identified using time-consuming methods such as visual examination of either the intact grain or one-dimensional electrophoretic patterns of the grain storage proteins. A fast method for identification of wheat (Triticum aestivum L.) varieties has previously been developed, which combines analysis of alcohol-soluble wheat proteins (gliadins) using matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry with neural networks. Here we have applied the same method for the identification of both barley (Hordeum vulgare L.) and rye (Secale cereale L.) varieties. For barley, 95% of the mass spectra were correctly classified. This is an encouraging result, since in earlier experiments only a grouping into subsets of varieties was possible. However, the method was not useful in the classification of rye, due to the strong similarity between mass spectra of different varieties.
Bloch
H A
HA
BioCentrum-DTU, Biochemistry and Nutrition, Søltofts Plads, Technical University of Denmark, Building 224, DK-2800 Kgs. Lyngby, Denmark.
Petersen
M
M
Sperotto
M M
MM
Keşmir
C
C
Radzikowski
L
L
Jacobsen
S
S
Søndergaard
I
I
D005983:Glutens; D001467:Hordeum / Q000737:chemistry* / Q000145:classification; D016571:Neural Networks, Computer; D010940:Plant Proteins / Q000032:analysis; D012434:Secale / Q000737:chemistry* / Q000145:classification; D013045:Species Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D010940:Plant Proteins; D005983:Glutens