Stable genetic transformation of Eschscholzia californica expressing synthetic green fluorescent proteins.
An efficient protocol is described for the stable genetic transformation of Eschscholzia californica (California poppy) using Agrobacterium tumefaciens as a vector. We have employed the disarmed A. tumefaciens LBA4404 encoding a synthetic green fluorescent protein reporter gene that is further controlled by an enhanced cauliflower mosaic virus 35S promoter. Stably transformed E. californica cells appear 3 weeks after initial cocultivation of A. tumefaciens with poppy leaves, stems, or roots. Transformed poppy calli were visualized by exposure to long-wave UV or blue light and analyzed in detail by fluorescent microscopy and laser-scanning microscopy. Moreover, green fluorescent calli have been maintained through continual subculture and grow well either on Gamborg's B5 agarose or liquid medium.
Lee
J
J
Department of Chemical and Biochemical Engineering, Rutgers, The State University of New Jersey, 98 Brett Road, Piscataway, New Jersey 08854-8058, USA.
Pedersen
H
H
D016960:Agrobacterium tumefaciens / Q000235:genetics; D017796:Caulimovirus / Q000235:genetics; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000235:genetics*; D010207:Papaver / Q000196:embryology / Q000235:genetics*; D010946:Plants, Medicinal; D011401:Promoter Regions, Genetic; D012639:Seeds; D014170:Transformation, Genetic
D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins