title: Growth phase variation in cell and nucleoid morphology in a Bacillus subtilis recA mutant.
abstract: The major role of RecA is thought to be in helping repair and restart stalled replication forks. During exponential growth, Bacillus subtilis recA cells exhibited few microscopically observable nucleoid defects. However, the efficiency of plating was about 12% of that of the parent strain. A substantial and additive defect in viability was also seen for addB and recF mutants, suggesting a role for the corresponding recombination paths during normal growth. Upon entry into stationary phase, a subpopulation (approximately 15%) of abnormally long cells and nucleoids developed in B. subtilis recA mutants. In addition, recA mutants showed a delay in, and a diminished capacity for, effecting prespore nucleoid condensation.
authors: Department of Microbiology and Immunology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140, USA.
authors: S A
authors: SA
authors: Sciochetti
authors: G W
authors: GW
authors: Blakely
authors: P J
authors: PJ
authors: Piggot
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D001426:Bacterial Proteins / Q000235:genetics; D002467:Cell Nucleus; D015169:Colony Count, Microbial; D004268:DNA-Binding Proteins / Q000235:genetics; D005090:Exodeoxyribonucleases; D005456:Fluorescent Dyes; D007211:Indoles; D009154:Mutation; D011938:Rec A Recombinases / Q000235:genetics / Q000378:metabolism*; D013171:Spores, Bacterial / Q000502:physiology
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D005456:Fluorescent Dyes; D007211:Indoles; C007293:DAPI; C066218:recF protein, Bacteria; D011938:Rec A Recombinases; D005090:Exodeoxyribonucleases; C080949:AddB protein, Bacillus subtilis