Integration of gene amplification and capillary gel electrophoresis on a polydimethylsiloxane-glass hybrid microchip.
We report on the development of a hybrid polydimethylsiloxane (PDMS)-glass microchip for genetic analysis by functional integration of polymerase chain reaction (PCR) and capillary gel electrophoresis (CGE), and on related temperature control systems for PCR on a PDMS-glass hybrid microchip. The microchip was produced by molding PDMS against a microfabricated master with comparatively simple and inexpensive methods. PCR was successfully carried out on the PDMS-glass hybrid microchip with 500 bp target of lambdaDNA and the amplified gene was subsequently analyzed by CGE on the same PDMS-glass microchip. The chip could be considered as an inexpensive single-use apparatus compared to glass or silicon-made microchips for the same purpose.
Hong
J W
JW
Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, Japan.
Fujii
T
T
Seki
M
M
Yamamoto
T
T
Endo
I
I
D010582:Bacteriophage lambda / Q000737:chemistry; D004279:DNA, Viral / Q000032:analysis; D004129:Dimethylpolysiloxanes; D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D005821:Genetic Techniques / Q000295:instrumentation; D005898:Glass; D007091:Image Processing, Computer-Assisted; D008832:Microchemistry / Q000295:instrumentation*; D008904:Miniaturization; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction / Q000295:instrumentation* / Q000379:methods; D012828:Silicones; D013696:Temperature
D004279:DNA, Viral; D004129:Dimethylpolysiloxanes; D012828:Silicones; C013830:baysilon