title: Inhibitors of platelet lipoxygenase from Ponkan fruit.
abstract: An activity-guided separation for inhibitors of rat platelet 12-lipoxygenase led to the isolation of two compounds, 4-O-feruloyl-5-O-caffeoylquinic acid (IC50; 5.5 microM) and methyl 4-O-feruloyl-5-O-caffeoylquinate (IC50; 1.9 microM) from the peel of Ponkan fruit (Citrus reticulata). The complete structure of each phenolic ester was determined by NMR spectroscopy [1H and 13C NMR spectra, 1H-1H correlation spectroscopy (COSY), 1H-detected heteronuclear multiple quantum coherence (HMQC), and heteronuclear multiple bond connectivity (HMBC) spectroscopies] and other spectral methods.
authors: Chugoku National Agricultural Experiment Station, Ministry of Agriculture, Forestry and Fisheries, Fukuyama, Hiroshima, Japan. ynogata@cgk.affrc.go.jp
authors: Y
authors: Y
authors: Nogata
authors: K
authors: K
authors: Sekiya
authors: H
authors: H
authors: Ohta
authors: K
authors: K
authors: Kusumoto
authors: T
authors: T
authors: Ishizu
mesh_terms: D000818:Animals; D001092:Arachidonate 12-Lipoxygenase / Q000097:blood*; D001792:Blood Platelets / Q000201:enzymology*; D002957:Citrus / Q000737:chemistry*; D003509:Cyclohexanecarboxylic Acids / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D016859:Lipoxygenase Inhibitors / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D015394:Molecular Structure; D051381:Rats; D017208:Rats, Wistar
chemical_list: C430222:4-O-feruloyl-5-O-caffeoylquinic acid; D003509:Cyclohexanecarboxylic Acids; D016859:Lipoxygenase Inhibitors; C430223:methyl 4-O-feruloyl-5-O-caffeoylquinate; D001092:Arachidonate 12-Lipoxygenase