title: Recognition of Yersinia enterocolitica multiple strain infection in twin infants using PCR-based DNA fingerprinting.
abstract: OBJECTIVE
  Yersinia enterocolitica causes several syndromes in humans. The most common presentation is enterocolitis in children, presenting as fever and diarrhoea. A Y. enterocolitica multiple strain infection in twin infants was investigated.
  
  
  RESULTS
  One isolate was recovered from one patient and two morphologically-different isolates were recovered from the other infant. Biochemically, all isolates were identified as Y. enterocolitica group. The genomic DNA from each strain was purified and DNA fingerprinting was performed. The banding patterns observed for Y. enterocolitica isolates 2 and 3, from patients 1 and 2, respectively, were identical when comparing the presence or absence of major bands. However, Y. enterocolitica isolate 1, from patient 1, showed a distinctive banding pattern from isolates 2 and 3.
  
  
  CONCLUSIONS
  The findings indicate that one infant was colonized by more than one strain of Y. enterocolitica, demonstrating that multiple strains can colonize and invade a patient.
  
  
  CONCLUSIONS
  Recognition of multiple strain infections can be important in diagnosis, treatment and prognosis of Y. enterocolitica infections, as well as in disease epidemiology. The technique described here offers a straightforward method for strain comparison.
authors: USDA, ARS, Antimicrobial Resistance Research Unit, Athens, GA 30605, USA. jgray@saa.ars.usda.gov
authors: J T
authors: JT
authors: Gray
authors: M
authors: M
authors: WaKabongo
authors: F E
authors: FE
authors: Campos
authors: A A
authors: AA
authors: Diallo
authors: C
authors: C
authors: Tyndal
authors: C A
authors: CA
authors: Tucker
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D015373:Bacterial Typing Techniques; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000032:analysis; D004200:Diseases in Twins; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction / Q000379:methods*; D019105:Random Amplified Polymorphic DNA Technique; D014427:Twins; D015009:Yersinia Infections / Q000382:microbiology*; D015008:Yersinia enterocolitica / Q000145:classification* / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial