title: Suppression of IgE responses in CD23-transgenic animals is due to expression of CD23 on nonlymphoid cells.
abstract: Serum IgE is suppressed in CD23-transgenic (Tg) mice where B cells and some T cells express high levels of CD23, suggesting that CD23 on B and T cells may cause this suppression. However, when Tg B lymphocytes were compared with controls in B cell proliferation and IgE synthesis assays, the two were indistinguishable. Similarly, studies of lymphokine production suggested that T cell function in the Tg animals was normal. However, adoptive transfer studies indicated that suppression was seen when normal lymphocytes were used to reconstitute Tg mice, whereas reconstitution of controls with Tg lymphocytes resulted in normal IgE responses, suggesting that critical CD23-bearing cells are irradiation-resistant, nonlymphoid cells. Follicular dendritic cells (FDC) are irradiation resistant, express surface CD23, and deliver iccosomal Ag to B cells, prompting us to reason that Tg FDC may be a critical cell. High levels of transgene expression were observed in germinal centers rich in FDC and B cells, and IgE production was inhibited when Tg FDCs were cultured with normal B cells. In short, suppressed IgE production in CD23-Tg mice appears to be associated with a population of radioresistant nonlymphoid cells. FDCs that interface with B cells in the germinal center are a candidate for explaining this CD23-mediated IgE suppression.
authors: Department of Microbiology and Immunology, Virginia Commonwealth University, Richmond, VA 23298, USA.
authors: M
authors: M
authors: Payet-Jamroz
authors: S L
authors: SL
authors: Helm
authors: J
authors: J
authors: Wu
authors: M
authors: M
authors: Kilmon
authors: M
authors: M
authors: Fakher
authors: A
authors: A
authors: Basalp
authors: J G
authors: JG
authors: Tew
authors: A K
authors: AK
authors: Szakal
authors: N
authors: N
authors: Noben-Trauth
authors: D H
authors: DH
authors: Conrad
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis; D020566:Dendritic Cells, Follicular / Q000276:immunology* / Q000378:metabolism* / Q000528:radiation effects; D015536:Down-Regulation / Q000235:genetics* / Q000276:immunology* / Q000528:radiation effects; D005786:Gene Expression Regulation / Q000276:immunology; D007073:Immunoglobulin E / Q000096:biosynthesis* / Q000097:blood; D008213:Lymphocyte Activation / Q000235:genetics; D017710:Lymphocyte Transfusion; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic / Q000276:immunology*; D011836:Radiation Tolerance / Q000276:immunology; D017455:Receptors, IgE / Q000096:biosynthesis* / Q000235:genetics* / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000528:radiation effects / Q000637:transplantation; D018417:Th1 Cells / Q000276:immunology / Q000378:metabolism; D018418:Th2 Cells / Q000276:immunology / Q000378:metabolism; D019076:Transgenes / Q000276:immunology
chemical_list: D016207:Cytokines; D017455:Receptors, IgE; D007073:Immunoglobulin E