Response of haemocyte lysosomes to bacterial inoculation in the oysters Ostrea edulis L. and Crassostrea gigas (Thunberg) and the scallop Pecten maximus (L).
Data are presented that demonstrate the application of the neutral red retention assay (NRR) to monitor the effects of a bacterial inoculation on the haemocyte lysosomes of the European flat oyster Ostrea edulis, Pacific oyster Crassostrea gigas and scallop Pecten maximus. Bivalves were acclimated to three temperature regimes (5, 15 and 25 degrees C), at constant salinity for 7 days in the laboratory. Once baseline responses to acclimation temperature had been established, the effects of an in vivo inoculation on haemocyte lysosomal stability were assessed using the NRR assay. Lysosomal membrane stability was reduced in the presence of bacteria for all three species of bivalve, but destabilisation of C. gigas haemocyte lysosomes appeared to be most sensitive to the presence of the bacterium Listonella anguillarum. For all three bivalve species, the reduction in lysosomal stability appeared to be proportional to the growth of the bacterial inoculate. Using appropriate controls, the NRR assay was demonstrated to have great potential as a tool with which to make rapid initial assessments of the immune status of bivalve molluscs.
Hauton
C
C
School of Ocean and Earth Sciences, Southampton Oceanography Centre, European Way, Southampton, Hants, UK. chris.hauton@millport.gla.ac.uk
Hawkins
L E
LE
Hutchinson
S
S
D000818:Animals; D006434:Hemocytes / Q000276:immunology* / Q000382:microbiology; D008247:Lysosomes / Q000276:immunology* / Q000382:microbiology; D008974:Mollusca / Q000276:immunology* / Q000382:microbiology; D009499:Neutral Red / Q000378:metabolism; D010125:Ostreidae / Q000276:immunology / Q000382:microbiology; D013696:Temperature; D014737:Vibrionaceae / Q000276:immunology*
D009499:Neutral Red