title: Screening from a subtracted embryonic chick hindbrain cDNA library: identification of genes expressed during hindbrain, midbrain and cranial neural crest development.
abstract: The vertebrate hindbrain is segmented into a series of transient structures called rhombomeres. Despite knowing several factors that are responsible for the segmentation and maintenance of the rhombomeres, there are still large gaps in understanding the genetic pathways that govern their development. To find previously unknown genes that are expressed within the embryonic hindbrain, a subtracted chick hindbrain cDNA library has been made and 445 randomly picked clones from this library have been analysed using whole mount in situ hybridisation. Thirty-six of these clones (8%) display restricted expression patterns within the hindbrain, midbrain or cranial neural crest and of these, twenty-two are novel and eleven encode peptides that correspond to or are highly related to proteins with previously uncharacterised roles during early neural development. The large proportion of genes with restricted expression patterns and previously unknown functions in the embryonic brain identified during this screen provides insights into the different types of molecules that have spatially regulated expression patterns in cranial neural tissue.
authors: Division of Developmental Neurobiology, National Institute for Medical Research, The Ridgeway, Mill Hill, NW7 1AA, London, UK.
authors: J H
authors: JH
authors: Christiansen
authors: E G
authors: EG
authors: Coles
authors: V
authors: V
authors: Robinson
authors: A
authors: A
authors: Pasini
authors: D G
authors: DG
authors: Wilkinson
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002642:Chick Embryo; D018076:DNA, Complementary / Q000378:metabolism*; D020224:Expressed Sequence Tags; D018507:Gene Expression Regulation, Developmental; D015723:Gene Library; D017403:In Situ Hybridization; D008636:Mesencephalon / Q000196:embryology*; D008969:Molecular Sequence Data; D009432:Neural Crest / Q000196:embryology*; D009693:Nucleic Acid Hybridization; D012313:RNA / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D012249:Rhombencephalon / Q000196:embryology*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger; D012313:RNA