Endogenous glutathione and platelet function in platelet concentrates stored in plasma or platelet additive solution.
Platelet function was studied in platelet concentrates by assay of the thrombin-induced release of endogenous serotonin and presence of the swirling phenomenon in relation to endogenous glutathione (GSH) and cysteine. In platelets stored in plasma, addition of cysteamine resulted in only a moderate fall in GSH after 5 days of storage, from an average of 14.91 to 11.46 nmol per 109 platelets. Exogenously added GSH had no effect, and addition of buthionine sulfoximine (BSO) resulted in almost complete depletion of GSH, to an average of 0.65 nmol per 109 platelets. Addition of cysteamine or GSH resulted in increased endogenous cysteine whereas BSO had no effect. In platelets stored in a platelet additive solution (T-sol), complete depletion of GSH was found in the presence of cysteamine, GSH and BSO. Endogenous serotonin was unchanged during storage both in plasma and in additive solution (2.8 nmol per 109 platelets). Despite almost total depletion of endogenous GSH, the thrombin-induced release of serotonin after 5 days' storage was significantly affected only in the presence of BSO in platelets stored in additive solution (mean values 72.3% vs. 63.3% of endogeneous serotonin, P < 0.05). Similarly, addition of cysteamine or GSH had no significant effect on swirling but BSO reduced the swirling score after 5 days' storage in platelet additive solution compared with plasma. After 10 days' storage, there was a significant reduction in swirling in the concentrates where BSO was added (P < 0.05).
Hervig
T
T
Blood Bank, University Hospital Haukeland, Bergen, Norway. tor.hervig@blod.haukeland.no
Mansoor
M A
MA
Farstad
M
M
D001792:Blood Platelets / Q000737:chemistry* / Q000378:metabolism; D001793:Blood Preservation / Q000379:methods* / Q000592:standards; D019328:Buthionine Sulfoximine / Q000494:pharmacology; D003543:Cysteamine / Q000494:pharmacology; D005978:Glutathione / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D010949:Plasma / Q000737:chemistry; D012701:Serotonin / Q000378:metabolism
D012701:Serotonin; D019328:Buthionine Sulfoximine; D003543:Cysteamine; D005978:Glutathione