Genotypic prevalence of apx1V in Actinobacillus pleuropneumoniae field isolates.
A total of 90 Actinobacillus pleuropneumoniae field strains from pigs were serotyped by slide agglutination and analyzed for the presence of the apxIV gene by polymerase chain reaction. Of the 90 isolates serotyped, serotypes 2 (47 isolates) and 5 (25 isolates) were the most common, followed by serotype 6 (10 isolates). Three isolates belonged to serotype 7, and 5 isolates could not be typed. All 90 A. pleuropneumoniae field isolates tested carried the apxIV gene. This gene is species specific rather than serotype specific. Therefore, the ApxIV toxin has potential value for use both in vaccines and in diagnostic tests.
Cho
W S
WS
Department of Veterinary Pathology, College of Veterinary Medicine, School of Agricultural Biotechnology, Seoul National University, Kyounggi Do, Republic of Korea.
Chae
C
C
D000189:Actinobacillus Infections / Q000453:epidemiology / Q000662:veterinary*; D016977:Actinobacillus pleuropneumoniae / Q000235:genetics* / Q000302:isolation & purification; D000818:Animals; D001426:Bacterial Proteins / Q000302:isolation & purification*; D017931:DNA Primers; D004587:Electrophoresis, Agar Gel / Q000662:veterinary; D005838:Genotype; D007723:Korea / Q000453:epidemiology; D011001:Pleuropneumonia / Q000453:epidemiology / Q000662:veterinary*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D015995:Prevalence; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology*
C122177:ApxIVA protein, Actinobacillus pleuropneumoniae; D001426:Bacterial Proteins; D017931:DNA Primers