title: Induction and characterization of hypoxanthine-phosphoribosyltransferase (Hprt-) deficient cell lines of Akodon cursor (Rodentia, Sigmodontinae).
abstract: Hypoxanthine-phosphoribosyltransferase negative (Hprt-) cell lines derived from an Akodon cursor liposarcoma were obtained by induced mutagenesis. All but one Hprt- cell line lacked Hprt mRNA transcripts while one (AKO 3) coded for a truncated protein. Cell fusion and karyotypic analyses showed that one cell line (AKO 1-15) could be successfully used for constructing hybrid panels and allow for a clear identification of human chromosomes in hybrid cells.
authors: Divisão de Genética, Instituto Nacional de Câncer, Rio de Janeiro, Brazil.
authors: C R
authors: CR
authors: Bonvicino
authors: M A
authors: MA
authors: Moreira
authors: R A
authors: RA
authors: Arcuri
authors: H N
authors: HN
authors: Seuánez
mesh_terms: D000782:Aneuploidy; D000818:Animals; D002459:Cell Fusion; D002875:Chromosomes / Q000235:genetics*; D006801:Humans; D006822:Hybrid Cells / Q000166:cytology* / Q000201:enzymology* / Q000378:metabolism; D007041:Hypoxanthine Phosphoribosyltransferase / Q000737:chemistry / Q000172:deficiency* / Q000235:genetics; D007621:Karyotyping; D008080:Liposarcoma / Q000201:enzymology / Q000235:genetics / Q000473:pathology; D008214:Lymphocytes / Q000166:cytology / Q000378:metabolism; D009115:Muridae / Q000235:genetics*; D016296:Mutagenesis / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017384:Sequence Deletion / Q000235:genetics; D014407:Tumor Cells, Cultured
chemical_list: D012333:RNA, Messenger; D007041:Hypoxanthine Phosphoribosyltransferase