title: Analysis of the healing response to gutta-percha and Diaket when used as root-end filling materials in periradicular surgery.
abstract: OBJECTIVE
  To analyse the healing response to gutta-percha and Diaket when used as root-end filling materials in periradicular surgery.
  
  
  METHODS
  Periradicular surgery was completed using the mandibular second, third and fourth premolar teeth from nine male mongrel dogs. The six roots on one side of the mouth were randomly allocated to one of the following groups: group A: a resected root end and a burnished gutta-percha root filling; group B: cavities were prepared to a depth of 4.0 mm, using ultrasonic root-end preparation and filled with Diaket. The response was evaluated histologically at 55 (nine specimens) and 150 (three specimens) days post operatively.
  
  
  RESULTS
  The data for the 55-day period was analysed statistically using Wilcoxon's Signed Ranks test. No statistical analysis was carried out on the 150-day group due to the small number of specimens. The level of significance was set at P < 0.05. No statistical significance was observed in the healing response between Diaket and gutta-percha in the following categories at 55 days: inflammatory response, angiogenesis, root-end resorption, and cementum deposition. Statistically significant differences were observed in the healing categories: bone apposition (P < 0.05) and periodontal ligament formation (P < 0.05).
  
  
  CONCLUSIONS
  At both time intervals, Diaket had a better healing response that was characterized by hard tissue formation adjacent to the root-end filling material bordered by occasional multinucleated giant cells. The nature of both the hard tissue formation and the adjacent cells, however, remains undetermined. Diaket displayed the best healing of either material used in this study.
authors: Department of Restorative Sciences, Graduate Endodontics, Baylor College of Dentistry, Texas A & M University System, Dallas, Texas, USA. dewspoon@tambed.edu
authors: D E
authors: DE
authors: Witherspoon
authors: J L
authors: JL
authors: Gutmann
mesh_terms: D000818:Animals; D001047:Apicoectomy; D001641:Bicuspid; D001729:Bismuth / Q000627:therapeutic use*; D003739:Dental Cementum / Q000473:pathology / Q000503:physiopathology; D003786:Dental Pulp Cavity / Q000473:pathology; D004285:Dogs; D004338:Drug Combinations; D005456:Fluorescent Dyes; D005500:Follow-Up Studies; D015726:Giant Cells / Q000473:pathology; D006171:Gutta-Percha / Q000627:therapeutic use*; D008297:Male; D018919:Neovascularization, Physiologic; D010513:Periodontal Ligament / Q000473:pathology / Q000503:physiopathology; D011145:Polyvinyls / Q000627:therapeutic use*; D011897:Random Allocation; D012184:Retrograde Obturation; D012387:Root Canal Filling Materials / Q000627:therapeutic use*; D012391:Root Resorption / Q000209:etiology; D018709:Statistics, Nonparametric; D014227:Triazines; D014464:Ultrasonic Therapy / Q000295:instrumentation; D014945:Wound Healing; D015034:Zinc Oxide / Q000627:therapeutic use*
chemical_list: D004338:Drug Combinations; D005456:Fluorescent Dyes; D011145:Polyvinyls; D012387:Root Canal Filling Materials; D014227:Triazines; C003213:Procion Brilliant Red M-2BS; C014457:bismuth phosphate, copolymers of vinyl acetate, dichlorophen, propionyl-acetophenone, triethanolamine, vinyl chloride, vinylisobutyl ether, zinc oxide drug combination; D006171:Gutta-Percha; D015034:Zinc Oxide; D001729:Bismuth