AIS overexpression in advanced esophageal cancer.
We examined AIS status in digestive tract cancers and found that all eight esophageal cancer cell lines (100%) showed AIS/TA-AIS gene overexpression, whereas 1 of 12 (8%) gastric cancer and 0 of 14 (0%) colon cancer cell lines showed AIS/TA-AIS gene expression. We then confirmed that the AIS gene, not the TA-AIS gene, was dominantly expressed in esophageal cancers by reverse transcription-PCR. AIS protein was also expressed in AIS gene-positive cell lines. Subsequently, we tested AIS gene expression in paired esophageal normal tissues and cancers. Twenty-five of 39 (64%) primary esophageal cancers demonstrated an obviously higher expression of AIS gene compared to paired normal tissues. Moreover, high AIS gene expression was significantly associated with lymph node metastases in esophageal cancer (P = 0.0271). These results suggested that AIS may be useful as a marker for advanced esophageal cancer.
Hibi
K
K
Second Department of Surgery, Nagoya University School of Medicine, Japan. khibi@tsuru.med.nagoya-u.ac.jp
Nakayama
H
H
Taguchi
M
M
Kasai
Y
Y
Ito
K
K
Akiyama
S
S
Nakao
A
A
D000368:Aged; D015152:Blotting, Northern; D015153:Blotting, Western; D002460:Cell Line; D003110:Colonic Neoplasms / Q000378:metabolism; D004938:Esophageal Neoplasms / Q000378:metabolism*; D005260:Female; D005799:Genes, Dominant; D016158:Genes, p53; D006801:Humans; D008297:Male; D008875:Middle Aged; D008957:Models, Genetic; D009363:Neoplasm Proteins; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000378:metabolism; D014018:Tissue Distribution; D014157:Transcription Factors; D015533:Transcriptional Activation; D014407:Tumor Cells, Cultured; D025521:Tumor Suppressor Proteins
D009363:Neoplasm Proteins; D011506:Proteins; C115051:TP63 protein, human; D014157:Transcription Factors; D025521:Tumor Suppressor Proteins