YopB of Yersinia enterocolitica is essential for YopE translocation.
A previous study has shown that YopB of Yersinia spp. is essential for translocation of Yop effectors across the eucaryotic plasma membrane (M.-P. Sory and G. R. Cornelis, Mol. Microbiol. 14:583--594, 1994). However, this role was recently challenged (V. T. Lee and O. Schneewind, Mol. Microbiol. 31:1619--1629, 1999). Using protease protection and digitonin extraction, we reconfirm that YopB of Yersinia enterocolitica is essential for the translocation of YopE into HeLa cell monolayers.
Nordfelth
R
R
Department of Cell and Molecular Biology, Umeå University, 901 87 Umeå, Sweden.
Wolf-Watz
H
H
D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism* / Q000502:physiology*; D001692:Biological Transport; D019286:Endopeptidase K / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology
D001425:Bacterial Outer Membrane Proteins; C078909:YopB protein, Yersinia; C063239:yopE protein, Yersinia; D012967:Sodium Dodecyl Sulfate; D019286:Endopeptidase K