Micromachined filter-chamber array with passive valves for biochemical assays on beads.
The filter-chamber array presented here enables a real-time parallel analysis of three different samples on beads in a volume of 3 nL, on a 1 cm2 chip. The filter-chamber array is a system containing three filter-chambers, three passive valves at the inlet channels and a common outlet. The design enables parallel sample handling and time-controlled analysis. The device is microfabricated in silicon and sealed with a Pyrex lid to enable real-time analysis. Single nucleotide polymorphism analysis by using pyrosequencing has successfully been performed in single filter-chamber devices. The passive valves consist of plasma-deposited octafluorocyclobutane and show a much higher resistance towards water and surface-active solutions than previous hydrophobic patches. The device is not sensitive to gas bubbles, clogging is rare and reversible, and the filter-chamber array is reusable. More complex (bio)chemical reactions on beads can be performed in the devices with passive valves than in the devices without valves.
Andersson
H
H
Royal Institute of Technology, Department of Signals, Sensors and Systems, Stockholm, Sweden. helene.andersson@s3.kth.se
van der Wijngaart
W
W
Stemme
G
G
D001671:Biochemistry / Q000295:instrumentation*; D003199:Computer Systems; D004277:DNA, Single-Stranded / Q000032:analysis; D004867:Equipment Design; D016158:Genes, p53; D008832:Microchemistry / Q000295:instrumentation*; D008863:Microspheres; D008904:Miniaturization; D016133:Polymerase Chain Reaction / Q000379:methods; D012212:Rheology; D012825:Silicon; D014462:Ultrafiltration / Q000295:instrumentation*
D004277:DNA, Single-Stranded; D012825:Silicon