Partial characterization of the N-linked oligosaccharide structures on P-selectin glycoprotein ligand-1 (PSGL-1).
PSGL-1, a specific ligand for P-, E- and L-selectin, was isolated from in vivo [3H]-glucosamine labeled HL-60 cells by a combination of wheat germ agglutinin-agarose and P- or E-selectin-agarose chromatography. N-linked oligosaccharides were released from the purified, denatured ligand molecule by peptide: N-glycosidase F treatment and, following separation by Sephacryl S-200 chromatography, partially characterized using lectin, ion-exchange and size-exclusion chromatography in combination with glycosidase digestions. The data obtained suggest that the N-glycans on PSGL-1 are predominantly core-fucosylated, multiantennary complex type structures with extended, poly-N-acetyllactosamine containing outer chains. A portion of the outer chains appears to be substituted with fucose indicating that the N-glycans, in addition to the O-glycans on PSGL-1, may be involved in selectin binding.
Aeed
P A
PA
Pharmacia Corp., Kalamazoo, MI 49001-0199, USA.
Geng
J G
JG
Asa
D
D
Raycroft
L
L
Ma
L
L
Elhammer
A P
AP
D002462:Cell Membrane / Q000737:chemistry* / Q000648:ultrastructure; D004798:Enzymes / Q000493:pharmacokinetics; D005643:Fucose / Q000737:chemistry; D018922:HL-60 Cells / Q000737:chemistry / Q000648:ultrastructure; D006801:Humans; D007962:Leukocytes / Q000737:chemistry* / Q000648:ultrastructure; D008562:Membrane Glycoproteins / Q000737:chemistry* / Q000648:ultrastructure; D009844:Oligosaccharides / Q000737:chemistry / Q000302:isolation & purification; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification*
D004798:Enzymes; D008562:Membrane Glycoproteins; D009844:Oligosaccharides; C084882:P-selectin ligand protein; D011134:Polysaccharides; D005643:Fucose