UV-light-dependent binding of a visual arrestin 1 isoform to photoreceptor membranes in a neuropteran (Ascalaphus) compound eye.
Arrestins are regulators of the active state of G-protein-coupled receptors. Towards elucidating the function of different arrestin subfamilies in sensory cells, we have isolated a novel arrestin 1, Am Arr1, from the UV photoreceptors of the neuropteran Ascalaphus macaronius. Am Arr1 forms a phylogenetic clade with antennal and visual Arr1 isoforms of invertebrates. Am Arr1 undergoes a light-dependent binding cycle to photoreceptor membranes, as reported earlier only for members of the arrestin 2 subfamily. This suggests a common control mechanism for the active state of invertebrate rhodopsins and G-protein-coupled receptors of antennal sensory cells. Furthermore, it implies that a strict correlation of distinct arrestin isoforms to distinct functions is not a general principle for invertebrate sensory cells.
Bentrop
J
J
Lehrstuhl für Zell- und Neurobiologie, Zoologisches Institut, Universität Karlsruhe, Haid-und-Neustr. 9, D-76131 Karlsruhe, Germany. joachim.bentrop@uni-karlsruhe.de
Schillo
M
M
Gerdon
G
G
Draslar
K
K
Paulsen
R
R
D000595:Amino Acid Sequence; D000818:Animals; D019390:Arrestins / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D019204:GTP-Binding Proteins / Q000378:metabolism; D017344:Genes, Insect; D066298:In Vitro Techniques; D007313:Insecta / Q000235:genetics / Q000378:metabolism; D007700:Kinetics; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism*; D017956:Photoreceptor Cells, Invertebrate / Q000378:metabolism* / Q000528:radiation effects; D010802:Phylogeny; D011485:Protein Binding / Q000528:radiation effects; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014466:Ultraviolet Rays
D019390:Arrestins; D010750:Phosphoproteins; D020033:Protein Isoforms; D011956:Receptors, Cell Surface; D019204:GTP-Binding Proteins