Atomic structure of the major capsid protein of rotavirus: implications for the architecture of the virion.
The structural protein VP6 of rotavirus, an important pathogen responsible for severe gastroenteritis in children, forms the middle layer in the triple-layered viral capsid. Here we present the crystal structure of VP6 determined to 2 A resolution and describe its interactions with other capsid proteins by fitting the atomic model into electron cryomicroscopic reconstructions of viral particles. VP6, which forms a tight trimer, has two distinct domains: a distal beta-barrel domain and a proximal alpha-helical domain, which interact with the outer and inner layer of the virion, respectively. The overall fold is similar to that of protein VP7 from bluetongue virus, with the subunits wrapping about a central 3-fold axis. A distinguishing feature of the VP6 trimer is a central Zn(2+) ion located on the 3-fold molecular axis. The crude atomic model of the middle layer derived from the fit shows that quasi-equivalence is only partially obeyed by VP6 in the T = 13 middle layer and suggests a model for the assembly of the 260 VP6 trimers onto the T = 1 viral inner layer.
Mathieu
M
M
Laboratoire de Génétique des Virus, CNRS-UPR 9053 1, Avenue de la Terrasse Bâtiment 14C, 91198 Gif-sur-Yvette Cedex, France.
Petitpas
I
I
Navaza
J
J
Lepault
J
J
Kohli
E
E
Pothier
P
P
Prasad
B V
BV
Cohen
J
J
Rey
F A
FA
D000595:Amino Acid Sequence; D000818:Animals; D000956:Antigens, Viral / Q000737:chemistry; D002213:Capsid / Q000737:chemistry*; D036022:Capsid Proteins; D002413:Cations, Divalent; D002417:Cattle; D018360:Crystallography, X-Ray; D006389:Hemagglutinins, Viral / Q000737:chemistry; D008958:Models, Molecular; D008969:Molecular Sequence Data; D017433:Protein Structure, Secondary; D012401:Rotavirus / Q000737:chemistry* / Q000648:ultrastructure; D017386:Sequence Homology, Amino Acid; D012997:Solvents; D014758:Viral Core Proteins / Q000737:chemistry; D014771:Virion / Q000737:chemistry / Q000648:ultrastructure; D015032:Zinc
D000956:Antigens, Viral; D036022:Capsid Proteins; D002413:Cations, Divalent; D006389:Hemagglutinins, Viral; D012997:Solvents; C061332:VP6 protein, Rotavirus; D014758:Viral Core Proteins; C066862:VP7 protein, orbivirus; D015032:Zinc