Catalytic heterogeneity of polyclonal DNA-hydrolyzing antibodies from the sera of patients with multiple sclerosis.
Various catalytic antibodies or abzymes have been detected recently in the sera of patients with several autoimmune pathologies, where their presence is most probably associated with autoimmunization. Recently we have shown that DNase activity is associated with IgGs from the sera of patients with multiple sclerosis (MS) but not with those from the sera of normal humans. Here we present evidence showing that MS IgG, its F(ab) fragments, and separated L-chains catalyze DNA hydrolysis. The properties of the DNase activity of these polyclonal IgGs distinguish them from other known human DNases. In addition, their specific activities with different oligonucleotide substrates and the range of optimal pHs, apparent K(M) values and substrate specificities varied widely for different patients. The findings speak in favor of the generation by the immune systems of individual patients of a variety of polyclonal catalytic IgG pools, from relatively small to extremely large ones.
Baranovskii
A G
AG
Novosibirsk Institute of Bioorganic Chemistry, Siberian Division of Russian Academy of Sciences, Lavrentieva Ave., 8, Novosibirsk 630090, Russian Federation.
Ershova
N A
NA
Buneva
V N
VN
Kanyshkova
T G
TG
Mogelnitskii
A S
AS
Doronin
B M
BM
Boiko
A N
AN
Gusev
E I
EI
Favorova
O O
OO
Nevinsky
G A
GA
D017151:Antibodies, Catalytic / Q000302:isolation & purification / Q000378:metabolism*; D002384:Catalysis; D004247:DNA / Q000378:metabolism*; D005090:Exodeoxyribonucleases / Q000302:isolation & purification / Q000378:metabolism*; D005092:Exonucleases / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007140:Immunoglobulin Fab Fragments / Q000302:isolation & purification / Q000378:metabolism*; D007074:Immunoglobulin G / Q000302:isolation & purification / Q000378:metabolism*; D007700:Kinetics; D009103:Multiple Sclerosis / Q000276:immunology*; D009838:Oligodeoxyribonucleotides / Q000378:metabolism; D043264:Phosphodiesterase I; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D013379:Substrate Specificity
D017151:Antibodies, Catalytic; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D009838:Oligodeoxyribonucleotides; D004247:DNA; D005090:Exodeoxyribonucleases; D005092:Exonucleases; C022650:spleen exonuclease; D010727:Phosphoric Diester Hydrolases; D043264:Phosphodiesterase I