title: Characterization of glutamate-gated chloride channels in the pharynx of wild-type and mutant Caenorhabditis elegans delineates the role of the subunit GluCl-alpha2 in the function of the native receptor.
abstract: Glutamate-gated chloride (GluCl) channels are the site of action of the anthelmintic ivermectin. Previously, the Xenopus laevis oocyte expression system has been used to characterize GluCl channels cloned from Caenorhabditis elegans. However, information on the native, pharmacologically relevant receptors is lacking. Here, we have used a quantitative pharmacological approach and intracellular recording techniques of C. elegans pharynx to characterize them. The glutamate response was a rapidly desensitizing, reversible, chloride-dependent depolarization (EC(50) = 166 microM), only weakly antagonized by picrotoxin. The order of potency of agonists was ibotenate > L-glutamate > kainate = quisqualate. Ivermectin potently and irreversibly depolarized the muscle (EC(50) = 2.7 nM). No further depolarization was seen with coapplication of maximal glutamate during the maximal ivermectin response, indicating that ivermectin depolarizes the muscle by the same ionic mechanism as glutamate (i.e., chloride). The potency of ivermectin on the pharynx was greater than at any of the GluCl subunits expressed in X. laevis oocytes. This effect of ivermectin was abolished in the mutant avr-15, which lacks a functional GluCl-alpha2 subunit. However, a chloride-dependent, nondesensitizing response to glutamate persisted. Therefore, the GluCl-alpha2 subunit confers ivermectin sensitivity and a high-affinity desensitizing glutamate response on the native pharyngeal GluCl receptor.
authors: Centre for Neuroscience, University of Southampton, Bassett Crescent East, Southampton UK.
authors: D J
authors: DJ
authors: Pemberton
authors: C J
authors: CJ
authors: Franks
authors: R J
authors: RJ
authors: Walker
authors: L
authors: L
authors: Holden-Dye
mesh_terms: D000818:Animals; D000969:Antinematodal Agents / Q000494:pharmacology; D017173:Caenorhabditis elegans / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D018118:Chloride Channels / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D018698:Glutamic Acid / Q000378:metabolism / Q000494:pharmacology*; D007559:Ivermectin / Q000494:pharmacology; D009154:Mutation; D009994:Osmolar Concentration; D010614:Pharynx / Q000187:drug effects / Q000378:metabolism; D017470:Receptors, Glutamate / Q000378:metabolism; D014162:Transfection; D014982:Xenopus laevis
chemical_list: D000969:Antinematodal Agents; D018118:Chloride Channels; D017470:Receptors, Glutamate; D018698:Glutamic Acid; D007559:Ivermectin