Protein synthesis during maturation of bovine oocytes, effect of epidermal growth factor.
The objective of this study was to determine whether epidermal growth factor (EGF) affected protein synthesis in oocytes during maturation. Initially, the effect of EGF on oocyte maturation was examined to ensure that there was a beneficial effect of EGF in the protein-free maturation medium used in these studies. Results showed that the presence of EGF during maturation significantly enhanced cleavage rate and development to the blastocyst stage. Development after maturation in the presence of EGF was similar to that seen in medium containing serum, luteinizing hormone, follicle-stimulating hormone and estradiol. Protein synthesis was examined in immature oocytes and after 16 or 24 h maturation. Oocytes from each group were labelled by incubation for 4 h with 35S-methionine, the proteins were then separated by two-dimensional sodium dodecyl sulphate-polyacrylamide gel electrophoresis. Between 400 and 500 proteins could be separated using this method and marked changes in protein synthesis was observed during maturation. Changes in eight different proteins were observed when protein patterns from oocytes matured for 16 h with and without EGF were compared. These results suggest that EGF plays a physiological role in oocyte maturation and identification of the proteins induced by EGF could be important for improving our understanding of oocyte maturation in vitro.
Goff
A K
AK
CRRA, Faculty of Veterinary Medicine, University of Montréal, St-Hyacinthe, Canada.
Yang
Z
Z
Cortvrindt
R
R
Smitz
J
J
Miron
P
P
D000818:Animals; D001755:Blastocyst / Q000187:drug effects / Q000502:physiology*; D002417:Cattle; D002478:Cells, Cultured; D015180:Electrophoresis, Gel, Two-Dimensional; D004815:Epidermal Growth Factor / Q000494:pharmacology / Q000502:physiology*; D005260:Female; D008540:Meiosis / Q000502:physiology; D009865:Oocytes / Q000187:drug effects / Q000502:physiology*; D014176:Protein Biosynthesis; D013462:Sulfur Radioisotopes
D013462:Sulfur Radioisotopes; D004815:Epidermal Growth Factor