Immunohistochemistry of bone sialoprotein and osteopontin during reparative dentinogenesis in vivo.
OBJECTIVE
To investigate the stage-specific and tissue expression of bone sialoprotein (BSP) and osteopontin (OPN) during reparative dentinogenesis in vivo.


METHODS
Direct pulp-capping with Ca(OH)2 developed a model used for the investigation of reparative dentinogenesis in the exposed dental pulp. In this model, standardized class V cavities were prepared close to the gingival margin on the buccal surface of each tooth. Animals were sacrificed 3, 7, and 10 days post-operation. Immunohistochemical staining determined the tissue-specific expression of BSP and OPN during the process.


RESULTS
Odontoblast-like cells and reparative dentin reacted positively with BSP and OPN. The expression of BSP reached a peak at day 7, then gradually decreased, while the expression of OPN showed no significant difference between day 7 and day 10.


CONCLUSIONS
BSP and OPN may play different roles in reparative dentinogenesis. BSP may serve as a nucleator of hydroxyapatite crystal formation.
Zhang
Q
Q
Department of Endodontics, Hubei Medical University (HMU), 65 Luoyu Road, Wuhan 430079, P. R. China.
Fan
M
M
Bian
Z
Z
Chen
Z
Z
Zhu
Q
Q
D000818:Animals; D002126:Calcium Hydroxide / Q000627:therapeutic use; D002448:Cell Adhesion / Q000502:physiology; D004396:Coloring Agents; D003460:Crystallization; D003737:Dental Cavity Preparation / Q000145:classification; D003785:Dental Pulp Capping; D003789:Dental Pulp Exposure / Q000628:therapy; D003809:Dentin, Secondary / Q000166:cytology / Q000502:physiology*; D003810:Dentinogenesis / Q000502:physiology; D004195:Disease Models, Animal; D004285:Dogs; D017886:Durapatite / Q000378:metabolism; D005500:Follow-Up Studies; D015870:Gene Expression; D007150:Immunohistochemistry; D058574:Integrin-Binding Sialoprotein; D009804:Odontoblasts / Q000166:cytology / Q000378:metabolism; D053495:Osteopontin; D010750:Phosphoproteins / Q000032:analysis*; D012795:Sialoglycoproteins / Q000032:analysis*
D004396:Coloring Agents; D058574:Integrin-Binding Sialoprotein; D010750:Phosphoproteins; D012795:Sialoglycoproteins; D053495:Osteopontin; D017886:Durapatite; D002126:Calcium Hydroxide