title: Cross-protection studies with gut membrane glycoprotein antigens from Haemonchus contortus and Teladorsagia circumcincta.
abstract: Gel profiles of the peanut and ConA lectin binding integral membrane glycoproteins of Teladorsagia circumcincta and Haemonchus contortus were compared and found to be considerably different. However, some of the Teladorsagia polypeptides were recognized by antisera specific for Haemonchus amino-, metallo- or aspartyl peptidases, enzymes which are known to be protective antigens for that parasite. As expected, an experimental vaccine containing these Haemonchus proteases was extremely effective against homologous challenge, reducing egg and worm counts by more than 99% and 92%, respectively, but it did not provide any useful cross-protection against either T. circumcincta, Trichostrongylus axei or Cooperiaoncophora. A reciprocal experiment, where sheep were immunized with the equivalent glycoproteins from T. circumcincta, showed that, while they were not protected against homologous challenge, there was some cross-protection against Haemonchus as measured by a significant reduction in worm egg output.
authors: Moredun Research Institute, Pentlands Science Park, Penicuik, Midlothian, UK.
authors: W D
authors: WD
authors: Smith
authors: D
authors: D
authors: Pettit
authors: S K
authors: SK
authors: Smith
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology*; D003429:Cross Reactions; D010450:Endopeptidases / Q000276:immunology; D006188:Haemonchiasis / Q000662:veterinary*; D006189:Haemonchus / Q000201:enzymology / Q000276:immunology* / Q000302:isolation & purification; D015801:Helminth Proteins / Q000276:immunology*; D007114:Immunization; D007413:Intestinal Mucosa / Q000737:chemistry / Q000469:parasitology; D007421:Intestine, Small / Q000469:parasitology; D037102:Lectins; D008562:Membrane Glycoproteins / Q000276:immunology*; D009348:Nematoda / Q000276:immunology* / Q000302:isolation & purification; D009349:Nematode Infections / Q000662:veterinary*; D010270:Parasite Egg Count; D012756:Sheep; D012757:Sheep Diseases / Q000469:parasitology / Q000517:prevention & control*; D013045:Species Specificity; D013997:Time Factors
chemical_list: D000947:Antigens, Helminth; D015801:Helminth Proteins; D037102:Lectins; D008562:Membrane Glycoproteins; D010450:Endopeptidases