Controlling transgene integration in plants.
The creation of transgenic plants has brought significant advances to light in plant biotechnology. However, in spite of the fact that transgenic plants are beginning to be grown widely, controlled transgene integration into a pre-determined site remains to be achieved. Here we suggest two alternative approaches for gene targeting in plants: manipulating the host and donor sequence, and targeting during active homologous recombination stages.
Kumar
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Federal Research Center for Forestry and Forest Products (BFH), Institute for Forest Genetics and Forest Tree Breeding, Sieker Land Str. 2, D-22927 Grosshansdorf, Germany. kumar@holz.uni-hamburg.de
Fladung
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D017360:Arabidopsis / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics*; D018390:Gene Targeting / Q000379:methods*; D005819:Genetic Markers; D018745:Genome, Plant; D009692:Nucleic Acid Heteroduplexes / Q000235:genetics; D009841:Oligonucleotides; D030821:Plants, Genetically Modified / Q000235:genetics*; D012231:Rhizobium / Q000235:genetics
D018744:DNA, Plant; D005819:Genetic Markers; D009692:Nucleic Acid Heteroduplexes; D009841:Oligonucleotides