Characterization of polar lipids of oral isolates of Candida, Pichia and Saccharomyces by Fast Atom Bombardment Mass Spectrometry (FAB MS).
OBJECTIVE
To characterize fatty acid and phospholipid analogue profiles of oral yeasts.


RESULTS
Twenty-seven strains of oral yeasts were cultured on SDA and lipids of freeze-dried cells were extracted and analysed by FAB MS. The most abundant carboxylate anion was m/z 281 (C18 : 1). The most intense phospholipid analogue ions were of PE, PG, PA and PI. Pichia etchellsii contained molecular species of PG and PE, whereas Saccharomyces cerevisiae had PA, PG and PE analogues. Mass spectra revealed that S. cerevisiae and Candida glabrata were distinct from one another and from the other species tested.


CONCLUSIONS
Oral yeasts largely differ with respect to their polar lipids. It is concluded that oral yeast species have distinctive fatty acid and phospholipid analogue anion profiles.


CONCLUSIONS
FAB MS provided novel chemotaxonomic information.
Mahmoudabadi
A Z
AZ
Oral Microbiology Laboratory, School of Biological Sciences, University of Manchester, Manchester M15 6FH, UK.
Boote
V
V
Drucker
D B
DB
D000838:Anions / Q000737:chemistry; D002175:Candida / Q000737:chemistry*; D002264:Carboxylic Acids / Q000737:chemistry; D005227:Fatty Acids / Q000032:analysis*; D006801:Humans; D009055:Mouth / Q000382:microbiology; D010743:Phospholipids / Q000032:analysis*; D010843:Pichia / Q000737:chemistry*; D012440:Saccharomyces / Q000737:chemistry*; D016339:Spectrometry, Mass, Fast Atom Bombardment / Q000379:methods
D000838:Anions; D002264:Carboxylic Acids; D005227:Fatty Acids; D010743:Phospholipids