M(R) 77 KDA factor derived from fibroblasts stimulates the invasion ability of breast-cancer cells.
Breast-cancer cells frequently invade into the skin. However, few reports have described the mechanisms responsible for this invasion. In this study, we investigated the effects of skin fibroblasts on the invasion ability of breast-cancer cells, using a modification of Boyden's chamber method, and purified an invasion-stimulating factor from fibroblasts. Conditioned medium of skin fibroblasts, DF-1, significantly stimulated the invasion ability of breast-cancer cells, OCUB-1. The invasion-stimulating factor produced from skin fibroblasts was then partially purified and characterized. The soluble invasion-stimulating factor of fibroblasts was a protein of 77 kDa, as calculated by SDS-PAGE. Although TGF-alpha and bFGF stimulated the invasion of OCUB-1, no inhibition of the 77 kDa factor was achieved with neutralizing antibodies against TGF-alpha and bFGF. These findings suggest that the 77 kDa factor derived from skin fibroblasts might be unique, might play an important role in invasion to the skin and might explain the frequent skin invasion in breast carcinoma.
Ikeda
K
K
First Department of Surgery, Osaka City University Medical School, 1-4-3 Asahimachi, Abeno-ku, Osaka, 545-8585 Japan.
Yashiro
M
M
Sawada
T
T
Hato
F
F
Hasuma
T
T
Ishikawa
T
T
Hirakawa-YSChung
K
K
D001685:Biological Factors / Q000302:isolation & purification / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000473:pathology*; D002460:Cell Line; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D017077:Culture Media, Conditioned / Q000494:pharmacology; D015194:Diffusion Chambers, Culture; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005347:Fibroblasts / Q000378:metabolism*; D006133:Growth Substances / Q000494:pharmacology; D006801:Humans; D008970:Molecular Weight; D009361:Neoplasm Invasiveness; D012867:Skin / Q000166:cytology; D014407:Tumor Cells, Cultured; D014462:Ultrafiltration
D001685:Biological Factors; D017077:Culture Media, Conditioned; D006133:Growth Substances