A disintegrin and metalloprotease with thrombospondin type1 motifs (ADAMTS-1) and IL-1 receptor type 1 mRNAs are simultaneously induced in nerve injured motor neurons.
Following rat hypoglossal nerve injury, expression of mRNAs for a disintegrin and metalloprotease with thrombospondin type1 motifs (ADAMTS-1) and IL-1 receptor type 1 (IL-1RT1) are induced in the injured motor neurons. Although N1E-115 (N1E) cells, which were treated with IL-1 alpha, showed no alteration of ADAMTS-1 mRNA expression, a substantial increase of ADAMTS-1 mRNA expression was observed in the N1E cells expressing IL-1RT1. These findings suggest that nerve injury promotes IL-1RT1 expression in the injured neurons and thereby ADAMTS-1 transcription was induced in response to IL-1 released from glial cells.
Sasaki
M
M
Department of Anatomy, Asahikawa Medical College, Midorigaoka-Higashi, Asahikawa, Hokkaido 078-8510, Japan.
Seo-Kiryu
S
S
Kato
R
R
Kita
S
S
Kiyama
H
H
D051722:ADAM Proteins; D000071097:ADAMTS1 Protein; D000818:Animals; D019771:Axotomy; D019483:Disintegrins / Q000235:genetics*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D007002:Hypoglossal Nerve / Q000166:cytology / Q000502:physiology; D007375:Interleukin-1 / Q000494:pharmacology; D008297:Male; D008666:Metalloendopeptidases / Q000235:genetics*; D009046:Motor Neurons / Q000502:physiology*; D009416:Nerve Regeneration / Q000187:drug effects / Q000502:physiology; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D017472:Receptors, Interleukin-1 / Q000235:genetics*
D019483:Disintegrins; D007375:Interleukin-1; D012333:RNA, Messenger; D017472:Receptors, Interleukin-1; D051722:ADAM Proteins; D000071097:ADAMTS1 Protein; C486324:Adamts1 protein, rat; D008666:Metalloendopeptidases; C407822:damage-induced neuronal endopeptidase