title: A monoclonal antibody against DNA binding helix of p53 protein.
abstract: Three monoclonal antibodies (Mabs) were generated against p53 DNA-binding core domain. When tested by immunoprecipitation, Western blot and immunofluorescence techniques, Mab 9E4, as well as 7D3 and 6B10 reacted with both wild-type and various mutant p53 proteins. The epitopes recognized by Mabs 7D3, 9E4 and 6B10 were located respectively within the amino acid residues 211-220, 281-290 and 291-300 of human p53 protein. The epitope recognized by 9E4 Mab coincides with helix 2, also called p53 DNA binding helix, which allows the direct contact of the protein with its target DNA sequences. This antibody may be useful to study transcription-dependent and transcription-independent activities of wild-type and mutant p53 proteins.
authors: Department of Molecular Biology and Genetics and BilGen Genetics and Biotechnology Center, Bilkent University, 06533, Ankara, Turkey.
authors: E
authors: E
authors: Yolcu
authors: B S
authors: BS
authors: Sayan
authors: T
authors: T
authors: Yağci
authors: R
authors: R
authors: Cetin-Atalay
authors: T
authors: T
authors: Soussi
authors: N
authors: N
authors: Yurdusev
authors: M
authors: M
authors: Ozturk
mesh_terms: D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D001665:Binding Sites / Q000276:immunology; D000939:Epitopes; D006801:Humans; D006825:Hybridomas; D010446:Peptide Fragments / Q000276:immunology; D017433:Protein Structure, Secondary; D016159:Tumor Suppressor Protein p53 / Q000737:chemistry / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D010446:Peptide Fragments; D016159:Tumor Suppressor Protein p53