title: CL22 - a novel cationic peptide for efficient transfection of mammalian cells.
abstract: Condensing peptide-DNA complexes have great potential as nonviral agents for gene delivery. To date, however, such complexes have given transfection activities greatly inferior to adenovirus and somewhat inferior to cationic lipid-DNA complexes, even for cell lines and primary cells in vitro. We report here the identification of a novel condensing peptide, CL22, which forms DNA complexes that efficiently transfect many cell lines, as well as primary dendritic and endothelial cells. We report studies with sequence and structure variants that define some properties of the peptide that contribute to efficient transfection. We demonstrate that the superior transfection activity of CL22 compared with other DNA condensing peptides is conferred at a step after uptake of the complexes into cells. We show that CL22-DNA complexes have transfection activity that is at least equivalent to the best available nonviral agents.
authors: Cobra Therapeutics Ltd, Keele, UK.
authors: A M
authors: AM
authors: Haines
authors: A S
authors: AS
authors: Irvine
authors: A
authors: A
authors: Mountain
authors: J
authors: J
authors: Charlesworth
authors: N A
authors: NA
authors: Farrow
authors: R D
authors: RD
authors: Husain
authors: H
authors: H
authors: Hyde
authors: H
authors: H
authors: Ketteringham
authors: R H
authors: RH
authors: McDermott
authors: A F
authors: AF
authors: Mulcahy
authors: T L
authors: TL
authors: Mustoe
authors: S C
authors: SC
authors: Reid
authors: M
authors: M
authors: Rouquette
authors: J C
authors: JC
authors: Shaw
authors: D R
authors: DR
authors: Thatcher
authors: J H
authors: JH
authors: Welsh
authors: D E
authors: DE
authors: Williams
authors: W
authors: W
authors: Zauner
authors: R O
authors: RO
authors: Phillips
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D018929:Cell Culture Techniques; D004247:DNA / Q000235:genetics; D003713:Dendritic Cells / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology; D005822:Genetic Vectors; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics*; D010455:Peptides / Q000737:chemistry / Q000235:genetics*; D014162:Transfection / Q000379:methods*; D014407:Tumor Cells, Cultured
chemical_list: D010446:Peptide Fragments; D010455:Peptides; C427249:cationic peptide, CL22; D004247:DNA