Alveolar epithelial barrier functions in ventilated perfused rabbit lungs.
We employed ultrasonic nebulization for homogeneous alveolar tracer deposition into ventilated perfused rabbit lungs. (22)Na and (125)I-albumin transit kinetics were monitored on-line with gamma detectors placed around the lung and the perfusate reservoir. [(3)H]mannitol was measured by repetitive counting of perfusion fluid samples. Volume of the alveolar epithelial lining fluid was estimated with bronchoalveolar lavage with sodium-free isosmolar mannitol solutions. Sodium clearance rate was -2.2 +/- 0.3%/min. This rate was significantly reduced by preadministration of ouabain/amiloride and enhanced by pretreatment with aerosolized terbutaline. The (125)I-albumin clearance rate was -0.40 +/- 0.05%/min. The appearance of [(3)H]mannitol in the perfusate was not influenced by ouabain/amiloride or terbutaline but was markedly enhanced by pretreatment with aerosolized protamine. An epithelial lining fluid volume of 1.22 +/- 0.21 ml was calculated in control lungs. Fluid absorption rate was 1.23 microl x g lung weight(-1) x min(-1), which was blunted after pretreatment with ouabain/amiloride. We conclude that alveolar tracer loading by aerosolization is a feasible technique to assess alveolar epithelial barrier properties in aerated lungs. Data on active and passive sodium flux, paracellular solute transit, and net fluid absorption correspond well to those in previous studies in fluid-filled lungs; however, albumin clearance rates were markedly higher in the currently investigated aerated lungs.
Ghofrani
H A
HA
Department of Internal Medicine, Justus-Liebig-University Giessen, 35385 Giessen, Germany.
Kohstall
M G
MG
Weissmann
N
N
Schmehl
T
T
Schermuly
R T
RT
Seeger
W
W
Grimminger
F
F
D000280:Administration, Inhalation; D000584:Amiloride / Q000008:administration & dosage; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D001993:Bronchodilator Agents / Q000008:administration & dosage; D004791:Enzyme Inhibitors / Q000008:administration & dosage; D005260:Female; D008168:Lung / Q000502:physiology*; D008297:Male; D008353:Mannitol / Q000008:administration & dosage / Q000493:pharmacokinetics; D009079:Mucociliary Clearance / Q000187:drug effects / Q000502:physiology; D009330:Nebulizers and Vaporizers; D010042:Ouabain / Q000008:administration & dosage; D010477:Perfusion; D010539:Permeability / Q000187:drug effects; D011433:Propranolol / Q000008:administration & dosage; D011479:Protamines / Q000008:administration & dosage; D011650:Pulmonary Alveoli / Q000378:metabolism*; D011817:Rabbits; D012121:Respiration, Artificial; D020545:Respiratory Mucosa / Q000378:metabolism*; D012711:Serum Albumin, Radio-Iodinated / Q000008:administration & dosage / Q000493:pharmacokinetics; D012979:Sodium Radioisotopes / Q000008:administration & dosage / Q000493:pharmacokinetics; D013726:Terbutaline / Q000008:administration & dosage; D014316:Tritium
D001993:Bronchodilator Agents; D004791:Enzyme Inhibitors; D011479:Protamines; D012711:Serum Albumin, Radio-Iodinated; D012979:Sodium Radioisotopes; D014316:Tritium; D008353:Mannitol; D010042:Ouabain; D000584:Amiloride; D011433:Propranolol; D013726:Terbutaline