Role of NF-Y in in vivo regulation of the gamma-globin gene.
The duplicated CCAAT box is required for gamma gene expression. We report here that the transcriptional factor NF-Y is recruited to the duplicated CCAAT box in vivo. A mutation of the duplicated CCAAT box that severely disrupts the NF-Y binding also reduces the accessibility level of the gamma gene promoter, affects the assembly of basal transcriptional machinery, and increases the recruitment of GATA-1 to the locus control region (LCR) and the proximal promoter and the recruitment of transcription cofactor CBP/p300 to the LCR. These findings suggest that recruitment of NF-Y to the duplicated CCAAT box plays a role in the chromatin opening of the gamma gene promoter as well as in the communication between the gamma gene promoter and the LCR.
Duan
Z
Z
Division of Medical Genetics, Department of Medicine, University of Washington, Seattle, Washington 98195, USA.
Stamatoyannopoulos
G
G
Li
Q
Q
D000818:Animals; D023081:CCAAT-Binding Factor / Q000378:metabolism / Q000502:physiology*; D002843:Chromatin; D005786:Gene Expression Regulation; D005914:Globins / Q000235:genetics*; D018922:HL-60 Cells; D006801:Humans; D020014:K562 Cells; D019901:Locus Control Region; D051379:Mice; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
D023081:CCAAT-Binding Factor; D002843:Chromatin; D005914:Globins