The effect of the source pressure on the abundance of ions of noncovalent protein assemblies in an electrospray ionization orthogonal time-of-flight instrument.
The effect of elevating the pressure in the interface region of an electrospray ionization orthogonal time-of-flight mass spectrometer on the ion intensity of different noncovalent protein assemblies has been investigated. Elevating the pressure in the interface region generally led to an enhanced detection of high m/z ions. The optimum pressure was found to be dependent on the m/z value of the ions. This pressure effect should be carefully addressed when relating ion abundance in the mass spectra to solution phase abundance of noncovalent protein assemblies.
Tahallah
N
N
Department of Biomolecular Mass Spectrometry, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Sorbonnelaan 16, 3584 CA Utrecht, The Netherlands.
Pinkse
M
M
Maier
C S
CS
Heck
A J
AJ
D015109:4-Hydroxybenzoate-3-Monooxygenase / Q000737:chemistry; D010407:Penicillium / Q000201:enzymology; D011312:Pressure; D011506:Proteins / Q000737:chemistry*; D011551:Pseudomonas fluorescens / Q000201:enzymology; D021241:Spectrometry, Mass, Electrospray Ionization
D011506:Proteins; D015109:4-Hydroxybenzoate-3-Monooxygenase