title: Interactive effects of elevated CO2 concentration and nitrogen supply on partitioning of newly fixed 13C and 15N between shoot and roots of pedunculate oak seedlings (Quercus robur).
abstract: Pedunculate oak (Quercus robur L.) seedlings were grown for 3 or 4 months (second- and third-flush stages) in greenhouses at two atmospheric CO2 concentrations ([CO2]) (350 or 700 micromol mol(-1)) and two nitrogen fertilization regimes (6.1 or 0.61 mmol N l(-1) nutrient solution). Combined effects of [CO2] and nitrogen fertilization on partitioning of newly acquired carbon (C) and nitrogen (N) were assessed by dual 13C and 15N short-term labeling of seedlings at the second- or third-flush stage of development. In the low-N treatment, root growth, but not shoot growth, was stimulated by elevated [CO2], with the result that shoot/root biomass ratio declined. At the second-flush stage, overall seedling biomass growth was increased (13%) by elevated [CO2] regardless of N fertilization. At the third-flush stage, elevated [CO2] increased growth sharply (139%) in the high-N but not the low-N treatment. Root/shoot biomass ratios were threefold higher in the low-N treatment relative to the high-N treatment. At the second-flush stage, leaf area was 45-51% greater in the high-N treatment than in the low-N treatment. At the-third flush stage, there was a positive interaction between the effects of N fertilization and [CO2] on leaf area, which was 93% greater in the high-N/elevated [CO2] treatment than in the low-N/ambient [CO2] treatment. Specific leaf area was reduced (17-25%) by elevated [CO2], whereas C and N concentrations of seedlings increased significantly in response to either elevated [CO2] or high-N fertilization. At the third-flush stage, acquisition of C and N per unit dry mass of leaf and fine root was 51 and 77% greater, respectively, in the elevated [CO2]/high-N fertilization treatment than in the ambient [CO2]/low-N fertilization treatment. However, there was dilution of leaf N in response to elevated [CO2]. Partitioning of newly acquired C and N between shoot and roots was altered by N fertilization but not [CO2]. More newly acquired C and N were partitioned to roots in the low-N treatment than in the high-N treatment.
authors: Unité d'Ecophysiologie, Forestière, INRA Nancy, Champenoux, France.
authors: P
authors: P
authors: Maillard
authors: J M
authors: JM
authors: Guehl
authors: J F
authors: JF
authors: Muller
authors: P
authors: P
authors: Gross
mesh_terms: D018533:Biomass; D002245:Carbon Dioxide / Q000378:metabolism / Q000502:physiology*; D002247:Carbon Isotopes; D009584:Nitrogen / Q000378:metabolism / Q000502:physiology*; D009587:Nitrogen Isotopes; D018517:Plant Roots / Q000378:metabolism / Q000502:physiology*; D018520:Plant Shoots / Q000378:metabolism / Q000502:physiology*; D029963:Quercus / Q000378:metabolism / Q000502:physiology*; D012987:Soil; D014197:Trees / Q000378:metabolism / Q000502:physiology*
chemical_list: D002247:Carbon Isotopes; D009587:Nitrogen Isotopes; D012987:Soil; D002245:Carbon Dioxide; D009584:Nitrogen