NreB from Achromobacter xylosoxidans 31A Is a nickel-induced transporter conferring nickel resistance.
There are two distinct nickel resistance loci on plasmid pTOM9 from Achromobacter xylosoxidans 31A, ncc and nre. Expression of the nreB gene was specifically induced by nickel and conferred nickel resistance on both A. xylosoxidans 31A and Escherichia coli. E. coli cells expressing nreB showed reduced accumulation of Ni(2+), suggesting that NreB mediated nickel efflux. The histidine-rich C-terminal region of NreB was not essential but contributed to maximal Ni(2+) resistance.
Grass
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Department of Soil, Water, and Environmental Science, University of Arizona, Tucson 85721, USA.
Fan
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Rosen
B P
BP
Lemke
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Schlegel
H G
HG
Rensing
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C
D000421:Alcaligenes / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D015152:Blotting, Northern; D002352:Carrier Proteins / Q000096:biosynthesis / Q000235:genetics*; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics; D005798:Genes, Bacterial; D008826:Microbial Sensitivity Tests; D016254:Mutagenesis, Insertional; D009532:Nickel / Q000494:pharmacology; D010957:Plasmids; D012329:RNA, Bacterial / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D011994:Recombinant Proteins / Q000096:biosynthesis; D014158:Transcription, Genetic
D002352:Carrier Proteins; D012329:RNA, Bacterial; D012333:RNA, Messenger; D011994:Recombinant Proteins; D009532:Nickel