The effect of latency-associated transcript on the herpes simplex virus type 1 latency-reactivation phenotype is mouse strain-dependent.
Herpes simplex virus type 1 (HSV-1) latency-associated transcript (LAT) null mutants reactivate poorly in the rabbit ocular model. The situation in mice is less clear. Reports concluding that LAT null mutants reactivate poorly in the mouse explant-induced reactivation (EIR) model are contradicted by a similar number of reports of normal EIR of LAT(-) mutants in mice. To determine if the EIR phenotype might be mouse strain-dependent we infected BALB/c and Swiss Webster mice with LAT(-) or LAT(+) virus and assessed EIR in individual trigeminal ganglia. Compared to LAT(+) virus, LAT(-) virus reactivated poorly in Swiss Webster mice (P<0.05). In contrast, the EIR phenotype of these viruses was similar in BALB/c mice (P>0.1). Thus, LAT appeared to have a much greater impact on the EIR phenotype in Swiss Webster mice than in BALB/c mice. The mouse strain therefore appeared consequential in the HSV-1 EIR phenotype in mice.
Perng
Guey-Chuen
GC
Ophthalmology Research Laboratories, Cedars-Sinai Medical Center Burns & Allen Research Institute, Davis Bldg Room 5072, 8700 Beverly Blvd, Los Angeles, CA 90048, USA1.
Slanina
Susan M
SM
Ophthalmology Research Laboratories, Cedars-Sinai Medical Center Burns & Allen Research Institute, Davis Bldg Room 5072, 8700 Beverly Blvd, Los Angeles, CA 90048, USA1.
Ghiasi
Homayon
H
Department of Ophthalmology, UCLA School of Medicine, Los Angeles, CA, USA2.|Ophthalmology Research Laboratories, Cedars-Sinai Medical Center Burns & Allen Research Institute, Davis Bldg Room 5072, 8700 Beverly Blvd, Los Angeles, CA 90048, USA1.
Nesburn
Anthony B
AB
Department of Ophthalmology, UCLA School of Medicine, Los Angeles, CA, USA2.|Ophthalmology Research Laboratories, Cedars-Sinai Medical Center Burns & Allen Research Institute, Davis Bldg Room 5072, 8700 Beverly Blvd, Los Angeles, CA 90048, USA1.
Wechsler
Steven L
SL
Department of Ophthalmology, UCLA School of Medicine, Los Angeles, CA, USA2.|Ophthalmology Research Laboratories, Cedars-Sinai Medical Center Burns & Allen Research Institute, Davis Bldg Room 5072, 8700 Beverly Blvd, Los Angeles, CA 90048, USA1.
D000818:Animals; D018259:Herpesvirus 1, Human / Q000235:genetics* / Q000472:pathogenicity; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D010641:Phenotype; D012333:RNA, Messenger; D012367:RNA, Viral; D017735:Virus Latency
D012333:RNA, Messenger; D012367:RNA, Viral