Expression of the apolipoprotein C-II gene during myelomonocytic differentiation of human leukemic cells.
Apolipoprotein C-II (apoC-II), which is known to activate lipoprotein lipase (LPL), was identified by ordered differential display (ODD)-polymerase chain reaction (PCR) as a cDNA fragment exhibiting a distinct increase in expression during 12-O-tetradecanoylphorbol 13-acetate (TPA)-induced differentiation of promonocytic U937 cells into monocytes and macrophages. The amount of apoC-II mRNA expression detectable in U937 cells significantly increased and reached a maximum 24-48 h after treatment with 32 nM TPA. apoC-II mRNA was also detected in monocytic THP-1 cells but was not detected in promyelocytic HL-60 cells. In healthy human tissues, the most significant expression of apoC-II mRNA was in the liver. Although apoC-II mRNA expression was markedly up-regulated during the induced differentiation of HL-60 cells into monocytes and macrophages with 32 nM TPA, such expression was not induced during the differentiation of HL-60 cells into granulocytes with 1.25% dimethyl sulfoxide. These results suggest that human apoC-II expression is induced at the transcription level during myelomonocytic differentiation and may confer an important role to macrophages involved in normal lipid metabolism and atherosclerosis.
Chun
E M
EM
Department of Microbiology, College of Natural Sciences, Kyungpook National University, Taegu, Korea.
Park
Y J
YJ
Kang
H S
HS
Cho
H M
HM
Jun
D Y
DY
Kim
Y H
YH
D016475:3T3 Cells / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D053304:Apolipoprotein C-II; D001056:Apolipoproteins C / Q000096:biosynthesis* / Q000235:genetics; D002277:Carcinoma / Q000473:pathology; D002454:Cell Differentiation; D019925:Cyclin A / Q000096:biosynthesis / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004789:Enzyme Activation; D020869:Gene Expression Profiling; D015973:Gene Expression Regulation, Leukemic / Q000187:drug effects*; D006098:Granulocytes / Q000166:cytology / Q000378:metabolism; D018922:HL-60 Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D019337:Hematologic Neoplasms / Q000473:pathology; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism; D019169:Jurkat Cells / Q000187:drug effects / Q000378:metabolism; D016547:Kinesins / Q000096:biosynthesis / Q000235:genetics; D050356:Lipid Metabolism; D008071:Lipoprotein Lipase / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008264:Macrophages / Q000166:cytology / Q000378:metabolism; D051379:Mice; D009000:Monocytes / Q000166:cytology / Q000378:metabolism*; D022423:Myeloid Cells / Q000187:drug effects / Q000378:metabolism; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis; D013382:Subtraction Technique; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014158:Transcription, Genetic / Q000187:drug effects; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism; D020298:U937 Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism
D053304:Apolipoprotein C-II; D001056:Apolipoproteins C; D019925:Cyclin A; D018076:DNA, Complementary; C091489:KIF2C protein, human; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D008071:Lipoprotein Lipase; D016547:Kinesins; D013755:Tetradecanoylphorbol Acetate; D004121:Dimethyl Sulfoxide