Inhibition of coxsackievirus B4 replication in stably transfected cells expressing human MxA protein.
Coxsackieviruses B (CVB) (B1-B6), positive-strand RNA viruses, cause a variety of diseases. CVB4 may have a causal role in insulin-dependent diabetes mellitus. IFN-alpha inhibits CVB replication; however, the mechanism is not well known. The interferon-alpha-inducible human MxA protein exerts an antiviral activity against negative-strand RNA viruses and against Semliki Forest virus, a positive-strand RNA virus. To test the antiviral spectrum of MxA against CVB4, we took advantage of stably transfected Vero cells expressing MxA (Vero/MxA) in 98% of cells. Compared with control cells, in Vero/MxA cells, CVB4 yields were dramatically reduced and expression of the VP1 CVB protein analyzed by immunofluorescence was highly restricted. Furthermore, the accumulation of positive- and negative-strand CVB4 RNA was prevented as shown by in situ hybridization and RT-PCR. These results indicate that the antiviral activity of MxA extends to CVB4 and that its replication cycle is inhibited at an early step in Vero/MxA cells.
Chieux
V
V
Laboratoire de Virologie, CHRU, Institut Gernez-Rieux, 59037 Lille Cedex, France.
Chehadeh
W
W
Harvey
J
J
Haller
O
O
Wattré
P
P
Hober
D
D
D000818:Animals; D000998:Antiviral Agents / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D002213:Capsid / Q000378:metabolism; D002522:Chlorocebus aethiops; D003384:Coxsackievirus Infections / Q000821:virology*; D029822:Enterovirus B, Human / Q000502:physiology*; D005455:Fluorescent Antibody Technique; D019204:GTP-Binding Proteins; D017403:In Situ Hybridization; D064547:Myxovirus Resistance Proteins; D011506:Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D012367:RNA, Viral / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection; D014709:Vero Cells; D014779:Virus Replication
D000998:Antiviral Agents; C579071:MX1 protein, human; D064547:Myxovirus Resistance Proteins; D011506:Proteins; D012367:RNA, Viral; D019204:GTP-Binding Proteins