Cytotoxicity effects of di- and tri-hydroxybenzaldehydes as a chemopreventive potential agent on tumor cells.
As part of our earlier search for new compounds with improved biological activities including antioxidant, anti-inflammatory, and tumor growth inhibition activities, we synthesized 2,4,5-trihydroxybenzaldehyde (2,4,5-THBA) from commercially available Sesamol. First we examined the free radical-quenching capacity of 2,4,5-THBA, 3,4-dihydroxybenzaldehyde (3,4-DHBA), and 2,5-dihydroxybenzaldehyde (2,5-DHBA) in vitro by 1,1-diphenyl-1,2-picryhydrazylradical (DPPH) test. The antioxidant bioactivity was also evaluated using the model of t-butyl hydroperoxide (t-BHP)-induced cytotoxicity in rat primary hepatocytes. Finally, three chemicals were tested by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay on human nasopharynx carcinoma cells (KB cells), human hepatoblastoma (HepG2), and human leukemia HL-60 cells. The 2,4,5-THBA shows significant cytotoxicity on HL-60 cells. The results suggest that 2,4,5-THBA may be a potential chemopreventor or chemotherapy agent against HL-60 cells.
Tseng
T H
TH
Institute of Biochemistry, Chung Shan Medical and Dental College, Taichung, Taiwan.
Tsheng
Y M
YM
Lee
Y J
YJ
D000818:Animals; D016588:Anticarcinogenic Agents / Q000138:chemical synthesis / Q000494:pharmacology / Q000633:toxicity*; D000975:Antioxidants / Q000138:chemical synthesis / Q000494:pharmacology; D001547:Benzaldehydes / Q000138:chemical synthesis / Q000494:pharmacology / Q000633:toxicity*; D005562:Formazans / Q000737:chemistry; D005609:Free Radicals / Q000737:chemistry; D018922:HL-60 Cells / Q000187:drug effects; D022781:Hepatocytes / Q000187:drug effects; D006801:Humans; D007624:KB Cells / Q000187:drug effects; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013778:Tetrazolium Salts / Q000737:chemistry
D016588:Anticarcinogenic Agents; D000975:Antioxidants; D001547:Benzaldehydes; D005562:Formazans; D005609:Free Radicals; D013778:Tetrazolium Salts; C079782:MTT formazan