title: Use of an immunoassay as a means to detect polychlorinated biphenyls in animal fat.
abstract: In this paper we describe the validation and utilization of the Hybrinzme DELFIA immunoassay for detecting polychlorinated biphenyls (PCBs) in animal fat. The immunoassay incorporates a rapid sample processing protocol that has been optimized to detect concentrations of PCBs in animal fat at or above 200 ng/g. The limit of detection of the assay was less than 50 ng/g. When the action level for the DELFIA assay was set at 100 ng/g to detect samples having 200 ng/g or more PCBs, the false negative rate was estimated at 0.2%. The false positive rate depends on the concentration of PCBs in the sample population and was estimated at 4% and 7% for samples having 10 ng/g and 20 ng/g PCBs, respectively. By utilizing the DELFIA assay system to select for positive samples and GC/MS analysis for confirmation analysis, an accurate and cost-effective programme has been established for the high throughput detection of PCBs in animal products.
authors: European Commission, DG Joint Research Centre, Institute for Health and Consumer Protection, Food Products and Consumer Goods Unit, Ispra, Italy.
authors: S
authors: S
authors: Jaborek-Hugo
authors: C
authors: C
authors: von Holst
authors: R
authors: R
authors: Allen
authors: T
authors: T
authors: Stewart
authors: J
authors: J
authors: Willey
authors: E
authors: E
authors: Anklam
mesh_terms: D000273:Adipose Tissue / Q000737:chemistry*; D000818:Animals; D002138:Calibration; D005188:False Negative Reactions; D005189:False Positive Reactions; D008401:Gas Chromatography-Mass Spectrometry; D007118:Immunoassay / Q000379:methods; D008460:Meat / Q000032:analysis*; D011078:Polychlorinated Biphenyls / Q000032:analysis*; D013552:Swine
chemical_list: D011078:Polychlorinated Biphenyls