title: Modification of an automated liquid-handling system for reagent-jet, nanoliter-level dispensing.
abstract: Reducing the scale of biochemical reactions is becoming commonplace. Examples include the screening of large libraries of chemical compounds or gene sequences. These applications demand the ability to transfer sub-microliter volumes of fluid. To this end, we have modified a Hamilton MICROLAB 2200 with high-speed solenoids and a liquid pressurization system to modulate volume delivery down to the nanoliter level. Additional modifications include the use of sapphire-tipped dispensing nozzles and a high-resolution substage to assist in the construction of DNA microarrays. Techniques for characterizing the dispensed volume are presented.
authors: Oak Ridge National Laboratory, Oak Ridge, TN, USA.
authors: J S
authors: JS
authors: Hicks
authors: B W
authors: BW
authors: Harker
authors: K L
authors: KL
authors: Beattie
authors: M J
authors: MJ
authors: Doktycz
mesh_terms: D000537:Aluminum Oxide; D004581:Electronics / Q000295:instrumentation; D015723:Gene Library; D008845:Microinjections / Q000295:instrumentation*; D008967:Molecular Biology / Q000295:instrumentation*; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation*; D012984:Software
chemical_list: D000537:Aluminum Oxide