Up-regulation of a novel mRNA (NY-CO-1) involved in the methyl 4-methoxy-3-(3-methyl-2-butenoyl) benzoate (VT1)-induced proliferation arrest of a non-small-cell lung carcinoma cell line (NSCLC-N6).
It is now well known that treatment of tumors, especially non-small-cell lung cancer (NSCLC), remains limited and it is urgent to develop strategies that target tumor cells and their genetic features. In this regard, our work is about genetic modifications arising in an in vitro NSCLC cell line after treatment with a chemical substance, methyl 4-methoxy-3-(3-methyl-2-butenoyl) benzoate (VT1). First, we showed that VT1 induces arrest of proliferation by blocking cells in the G1 phase of the cell cycle. Second, we use "differential display" strategy to clarify the genetic mechanisms involved in this proliferation arrest. A novel mRNA, NY-CO-1 (New-York Colon 1), of unknown function showed up-regulated expression after treatment. Application of "antisense" strategy confirmed this novel mRNA induction was effectively linked to growth arrest. Therefore, these data provide new information about mechanisms participating in arrest of proliferation of tumor cells and open new ways of treatment to target tumor growth.
Carbonnelle
D
D
ISOMer (Institut des substances et organismes de la mer), Laboratoire de Pharmacologie Marine, Faculté de Pharmacie, Nantes, France.
Jacquot
C
C
Lanco
X
X
Le Dez
G
G
Tomasoni
C
C
Briand
G
G
Tsotinis
A
A
Calogeropoulou
T
T
Roussakis
C
C
D000475:Alkenes / Q000494:pharmacology*; D000951:Antigens, Neoplasm / Q000235:genetics* / Q000378:metabolism; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001565:Benzoates / Q000494:pharmacology*; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000473:pathology; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D048429:Cell Size / Q000187:drug effects; D004268:DNA-Binding Proteins / Q000032:analysis; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D029543:Nucleocytoplasmic Transport Proteins; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D051716:Replication Protein A; D014407:Tumor Cells, Cultured; D015854:Up-Regulation
D000475:Alkenes; D000951:Antigens, Neoplasm; D000970:Antineoplastic Agents; D001565:Benzoates; D004268:DNA-Binding Proteins; C402404:NEMF protein, human; D029543:Nucleocytoplasmic Transport Proteins; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; C497167:RPA1 protein, human; D051716:Replication Protein A; C423823:methyl-4-methoxy-3-(3-methyl-2-butenoyl) benzoate