Isolation and initial characterization of the mouse Dnmt3l gene.
We have isolated and sequenced the mouse zinc finger gene, Dnmt3l (DNA cytosine-5-methyltransferase 3-like), on mouse chromosome 10, showing similarity to members of the DNMT3/Dnmt3 family. The Dnmt3l protein contains an ADD zinc finger, which Dnmt3l shares with other Dnmt3 family members and Atrx. RT-PCR analysis showed Dnmt3l expression in testis, thymus, ovary, and heart, as well as in 7-day, 15-day, and 17-day mouse embryos.
Aapola
U
U
Institute of Medical Technology, University of Tampere, and Tampere University Hospital, Tampere, Finland. ulla.aapola@uta.fi
Lyle
R
R
Krohn
K
K
Antonarakis
S E
SE
Peterson
P
P
D000595:Amino Acid Sequence; D000818:Animals; D019556:COS Cells; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000032:analysis / Q000737:chemistry / Q000235:genetics*; D004622:Embryo, Mammalian / Q000201:enzymology; D005091:Exons / Q000235:genetics; D020224:Expressed Sequence Tags; D005260:Female; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D010053:Ovary / Q000201:enzymology; D020161:Physical Chromosome Mapping; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013737:Testis / Q000201:enzymology; D013950:Thymus Gland / Q000201:enzymology; D016335:Zinc Fingers
D012333:RNA, Messenger; C498796:Dnmt3l protein, mouse; D004248:DNA (Cytosine-5-)-Methyltransferases