Superantigen recognition by gammadelta T cells: SEA recognition site for human Vgamma2 T cell receptors.
Human gammadelta T cells expressing the Vgamma2Vdelta2 antigen receptors recognize nonpeptide prenyl pyrophosphate and alkylamine antigens. We find that they also recognize staphylococcal enterotoxin A superantigens in a manner distinct from the recognition of nonpeptide antigens. Using chimeric and mutant toxins, SEA amino acid residues 20-27 were shown to be required for gammadelta TCR recognition of SEA. Residues at 200-207 that are critical for specific alphabeta TCR recognition of SEA do not affect gammadelta TCR recognition. SEA residues 20-27 are located in an area contiguous with the binding site of V beta chains. This study defines a superantigen recognition site for a gammadelta T cell receptor and demonstrates the differences between Vgamma2Vdelta2+ T cell recognition of superantigens and nonpeptide antigens.
Morita
C T
CT
Division of Rheumatology, Department of Internal Medicine, University of Iowa College of Medicine, Iowa City, IA 52242, USA. craig.morita@uiowa.edu
Li
H
H
Lamphear
J G
JG
Rich
R R
RR
Fraser
J D
JD
Mariuzza
R A
RA
Lee
H K
HK
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000494:pharmacology; D017951:Antigen Presentation; D000938:Antigen-Presenting Cells / Q000276:immunology; D001665:Binding Sites; D005075:Biological Evolution; D002460:Cell Line; D002999:Clone Cells / Q000276:immunology / Q000378:metabolism; D004768:Enterotoxins / Q000737:chemistry / Q000276:immunology*; D006681:HLA-D Antigens / Q000276:immunology; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011106:Polyisoprenyl Phosphates / Q000737:chemistry / Q000276:immunology; D011487:Protein Conformation; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000737:chemistry / Q000276:immunology; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000737:chemistry* / Q000276:immunology*; D013379:Substrate Specificity; D018089:Superantigens / Q000737:chemistry / Q000276:immunology*; D013601:T-Lymphocytes / Q000737:chemistry / Q000276:immunology*
D000911:Antibodies, Monoclonal; D004768:Enterotoxins; D006681:HLA-D Antigens; D011106:Polyisoprenyl Phosphates; D016693:Receptors, Antigen, T-Cell, alpha-beta; D016692:Receptors, Antigen, T-Cell, gamma-delta; D018089:Superantigens; C026066:enterotoxin A, Staphylococcal