title: Differences detected in vivo between samples of aflatoxin-contaminated peanut meal, following decontamination by two ammonia-based processes.
abstract: A sample of peanut meal, highly contaminated with aflatoxins, has been subjected to decontamination by two commercial ammonia-based processes. The original contaminated and the two decontaminated meals were fed to rats for 90 days. No lesions associated with aflatoxin-induced hepatocarcinogenesis were detected histologically following feeding with the two detoxified meals. There were, however, clear differences between the two meals in respect of growth rates of the rats. In addition, feeding one of the detoxified meals resulted in hepatic abnormalities detected using novel immunohistochemical reagents. Differences between the two detoxified meals were also indicated by the results of studies using meals 'spiked' with [14C]-aflatoxin B1 prior to being subjected to the detoxification processes. The meals differed in the bioavailability of the label. It was concluded that peanut meal where an initial, unacceptable level of contamination with aflatoxins had been reduced by two ammonia-based processes to comparable, acceptable levels, may still have different effects in vivo when incorporated into animal diets.
authors: MRC Toxicology Unit, University of Leicester, UK. neal@merstham95.fsnet.co.uk
authors: G E
authors: GE
authors: Neal
authors: D J
authors: DJ
authors: Judah
authors: P
authors: P
authors: Carthew
authors: A
authors: A
authors: Verma
authors: I
authors: I
authors: Latour
authors: L
authors: L
authors: Weir
authors: R D
authors: RD
authors: Coker
authors: M J
authors: MJ
authors: Nagler
authors: L A
authors: LA
authors: Hoogenboom
mesh_terms: D000348:Aflatoxins / Q000493:pharmacokinetics / Q000633:toxicity*; D000641:Ammonia; D000818:Animals; D010367:Arachis; D001682:Biological Availability; D003666:Decontamination / Q000379:methods; D004435:Eating / Q000187:drug effects; D005506:Food Contamination; D007124:Immunoenzyme Techniques; D007668:Kidney / Q000473:pathology; D008099:Liver / Q000473:pathology; D008113:Liver Neoplasms / Q000139:chemically induced; D008297:Male; D009929:Organ Size / Q000187:drug effects; D051381:Rats; D011916:Rats, Inbred F344; D015430:Weight Gain / Q000187:drug effects
chemical_list: D000348:Aflatoxins; D000641:Ammonia