Involvement of the neurotensin receptor subtype NTR3 in the growth effect of neurotensin on cancer cell lines.
The expression of the 3 currently known neurotensin receptors was studied in human cancer cells of prostatic, colonic or pancreatic origin by means of RT-PCR analysis and binding experiments. All the cells selected for this work have been shown to exhibit a growth response to neurotensin. We found that the 7 transmembrane domain, levocabastine insensitive receptor (NTR1) is expressed in most but not all of the cells studied whereas the 7 transmembrane domain, levocabastine sensitive receptor (NTR2) is present in none of these cells. The 100 kDa-type I neurotensin receptor (NTR3) is expressed in all the cells assayed. Moreover, we demonstrated that neurotensin can stimulate the growth of CHO cells stably transfected with the NTR3. Taken together, our results strongly suggest that the NTR3 subtype could be involved in the growth response of human cancer cells to neurotensin.
Dal Farra
C
C
Institut de Pharmacologie Moléculaire et Cellulaire, Sophia-Antipolis, Valbonne, France.
Sarret
P
P
Navarro
V
V
Botto
J M
JM
Mazella
J
J
Vincent
J P
JP
D000818:Animals; D016466:CHO Cells; D002462:Cell Membrane / Q000378:metabolism; D002793:Cholic Acids / Q000494:pharmacology; D003110:Colonic Neoplasms / Q000378:metabolism; D006224:Cricetinae; D019008:Drug Resistance, Neoplasm; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007700:Kinetics; D008297:Male; D009496:Neurotensin / Q000378:metabolism* / Q000494:pharmacology*; D010190:Pancreatic Neoplasms / Q000378:metabolism; D010880:Piperidines / Q000494:pharmacology; D011471:Prostatic Neoplasms / Q000378:metabolism; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000378:metabolism; D018028:Receptors, Neurotensin / Q000096:biosynthesis* / Q000737:chemistry / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D014162:Transfection; D014407:Tumor Cells, Cultured
D002793:Cholic Acids; C490985:NTSR2 protein, human; D010880:Piperidines; D012333:RNA, Messenger; D018028:Receptors, Neurotensin; C426065:neurotensin receptor NTR-3; C114036:neurotensin type 1 receptor; D009496:Neurotensin; C047340:levocabastine; C028213:3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate