MedB-1, a human tumor cell line derived from a primary mediastinal large B-cell lymphoma.
Primary mediastinal B-cell lymphoma is a locally highly aggressive but poorly disseminating tumor composed of medium sized or large cells most probably of thymic medullary origin. It has a mature B-cell phenotype, typically lacks immunoglobulin expression and has variable defects in expression of HLA-molecules. We present here a cell line, MedB-1, derived from such a tumor. As is frequently found in mediastinal B-cell lymphomas in situ, MedB-1 is CD10(-), CD19(+), CD21(-), CD22(+), CD23(+), CD25(-), CD37(+), CD38(-), CD39(+), CD40(+), CD54(+), CD95(+). Like the parental tumor, MedB-1 lacks HLA-A,B,C alpha-chains and beta(2)microglobulin and expresses HLA-D molecules at decreased levels. Both parental tumor and MedB-1 cells are clonally related as shown by immunoglobulin heavy chain gene rearrangement analysis. Unlike the parental tumor tissue, the MedB-1 cell line cytoplasmically expresses IgG/kappa in a very small subset of cells under standard culture conditions. MedB-1 does not contain any Epstein-Barr virus DNA. In a tissue adhesion assay MedB-1 cells showed an extensive binding to the medullary region of normal thymus. Altogether, MedB-1 is a suitable tool for functional and molecular analysis of this distinct lymphoma entity.
Möller
P
P
Institute of Pathology, University of Ulm, Ulm, Germany. peter.moeller@medizin.uni-ulm.de
Brüderlein
S
S
Sträter
J
J
Leithäuser
F
F
Hasel
C
C
Bataille
F
F
Moldenhauer
G
G
Pawlita
M
M
Barth
T F
TF
D001402:B-Lymphocytes / Q000276:immunology / Q000473:pathology* / Q000821:virology; D015139:Blotting, Southern; D002448:Cell Adhesion; D004279:DNA, Viral / Q000032:analysis; D015321:Gene Rearrangement; D004854:Herpesvirus 4, Human / Q000302:isolation & purification; D006801:Humans; D007136:Immunoglobulins / Q000096:biosynthesis; D016130:Immunophenotyping; D016403:Lymphoma, Large B-Cell, Diffuse / Q000276:immunology / Q000473:pathology* / Q000821:virology; D008479:Mediastinal Neoplasms / Q000276:immunology / Q000473:pathology* / Q000821:virology; D014407:Tumor Cells, Cultured
D004279:DNA, Viral; D007136:Immunoglobulins