Bovine papillomavirus oncoprotein E5 affects the arachidonic acid metabolism in cells.
The bovine papillomavirus type 1 (BPV-1) oncoprotein encoded by the E5 ORF is a small highly hydrophobic protein, which is capable of inducing oncogenic transformation of cells. We studied the effect of the BPV-1 E5 protein expression on the arachidonic acid metabolism in monkey (COS1) and human (C33A) cells. At relatively low protein concentrations the phospholipase A(2) (PLA(2)) activity and the arachidonic acid (AA) metabolism are activated. E5 mutant proteins, lacking cysteines responsible for the dimerisation of the protein (C37S, C37SC39S), and truncated E5, lacking the C-terminal region, are non-transforming and unable to stimulate the PLA(2) activity and AA metabolism. The transformation-defective mutant D33V, which does not activate the platelet-derived growth factor receptor (PDGFR), activates AA metabolism like wt E5. Our data suggest that the BPV-1 E5 protein could stimulate the AA metabolism independently of PDGF receptor.
Väli
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U
Institute of Molecular and Cell Biology, Tartu University, 23 Riia Street, Tartu, 51010, Estonia.
Kilk
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A
Ustav
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M
D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism*; D019556:COS Cells / Q000378:metabolism / Q000821:virology; D002472:Cell Transformation, Viral / Q000502:physiology; D002478:Cells, Cultured; D004789:Enzyme Activation / Q000502:physiology; D015870:Gene Expression / Q000235:genetics; D006801:Humans; D016297:Mutagenesis, Site-Directed / Q000235:genetics; D009856:Oncogene Proteins, Viral / Q000235:genetics / Q000378:metabolism*; D010741:Phospholipases A / Q000378:metabolism*; D017479:Receptors, Platelet-Derived Growth Factor / Q000378:metabolism; D014162:Transfection
D009856:Oncogene Proteins, Viral; C054304:oncogene protein E5, Bovine papillomavirus type 1; D016718:Arachidonic Acid; D017479:Receptors, Platelet-Derived Growth Factor; D010741:Phospholipases A