Inhibitory activities of lansoprazole against respiration in Helicobacter pylori.
Lansoprazole and its derivative AG-1789 dose-dependently inhibited cellular respiration by an endogenous substrate and decreased the ATP level in Helicobacter pylori cells. The inhibitory action of lansoprazole and AG-1789 against respiration was specific to substrates such as pyruvate and alpha-ketoglutarate and similar to the inhibitory action of rotenone, which is an inhibitor for the mitochondrial respiratory chain. Growth inhibition by lansoprazole and AG-1789 as well as by rotenone was augmented at high oxygen concentrations under atmospheric conditions. Since the 50% inhibitory concentrations of these compounds for the respiration were close to their MICs for H. pylori growth, the growth inhibition might be due to respiratory inhibition by these compounds.
Nagata
K
K
Department of Bacteriology, Hyogo College of Medicine, Nishinomiya, Hyogo 663-8501, Japan. kunagata@hyo-med.ac.jp
Sone
N
N
Tamura
T
T
D053799:2-Pyridinylmethylsulfinylbenzimidazoles; D000255:Adenosine Triphosphate / Q000378:metabolism; D000897:Anti-Ulcer Agents / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D016480:Helicobacter pylori / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D006801:Humans; D064747:Lansoprazole; D008826:Microbial Sensitivity Tests; D009853:Omeprazole / Q000031:analogs & derivatives / Q000494:pharmacology*; D010100:Oxygen / Q000378:metabolism
D053799:2-Pyridinylmethylsulfinylbenzimidazoles; D000897:Anti-Ulcer Agents; D064747:Lansoprazole; D000255:Adenosine Triphosphate; D009853:Omeprazole; D010100:Oxygen