title: Plasmodium vivax and Plasmodium chabaudi: intraerythrocytic traffic of antigenically homologous proteins involves a brefeldin A-sensitive secretory pathway.
abstract: We have used a monoclonal antibody (mAb 7C5B71) raised against the erythrocytic stages of Plasmodium vivax to identify a 148-kDa P vivax protein antigen (Pv-148) which crossreacts with an antigenically homologous 190-kDa protein of P. chabaudi (Pc-190). During parasite intraerythrocytic development Pv-148 and Pc-190 are exported into the host cell cytosol and become located in the surface membrane of the infected erythrocyte. Immunofluorescence confocal microscopy and immunoelectron microscopy studies showed that both Pv-148 and Pc-190 are released from the parasite and exported to the host cell cytoplasm in association with tubovesicular membrane (TVM) structures. Fluorescent in vivo labelling of P. chabaudi with Bodipy-ceramide followed by immunofluorescence staining with the mAb supported the association of antigenically homologous Pc-190 with TVM structures. In the presence of brefeldin A (BFA), secretion of antigenically homologous Pc-190 into the host cell cytoplasm was inhibited and the antigen remained in the parasite cytoplasm. BFA also arrested the maturation of the parasite. Taken together these results suggest that Pv-148 and Pc-190 are related parasite proteins that are transported into the host cell through a BFA-sensitive secretory pathway.
authors: Instituto Venezolano de Investigaciones Científicas IVIC, Caracas, Venezula. cbrocho@pasteur.ivic.ve
authors: C
authors: C
authors: Bracho
authors: I
authors: I
authors: Dunia
authors: M
authors: M
authors: Romano
authors: E L
authors: EL
authors: Benedetti
authors: H A
authors: HA
authors: Perez
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000378:metabolism*; D020126:Brefeldin A / Q000494:pharmacology*; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism* / Q000469:parasitology; D006801:Humans; D016788:Plasmodium chabaudi / Q000378:metabolism*; D010966:Plasmodium vivax / Q000254:growth & development / Q000378:metabolism*; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology*
chemical_list: D000953:Antigens, Protozoan; D011500:Protein Synthesis Inhibitors; D020126:Brefeldin A