title: Intrastriatal targets of projection fibers from the central lateral nucleus of the rat thalamus.
abstract: We examined light and electron microscopically intrastriatal targets of projection fibers from the central lateral thalamic nucleus (CL), which is a major relay of cerebello-striatal projections. The study was done in the rat by combining the anterograde tract-tracing with immunohistochemistry for parvalbumin (PV); an anterograde tracer (biotin dextran amine: BDA) was injected into the CL. In the striatum, 91% of BDA-labeled axon terminals made asymmetrical synapses on PV immunonegative dendritic spines (assumed to be those of striatal projection neurons); only 0.5% of BDA-labeled axon terminals made synapses on PV immunopositive dendritic shafts. The remaining BDA-labeled axon terminals were in synaptic contact with PV immunonegative dendritic shafts. The results suggest that the cerebello-striatal projections through the CL predominantly access to striatal projection neurons, with only minor access to PV immunopositive (assumed to be GABAergic) interneurons in the striatum.
authors: Department of Anatomy, Hirosaki University, School of Medicine, 5 Zaifucho, 036-8562, Hirosaki, Japan. ichinohe@infoaomori.ne.jp
authors: N
authors: N
authors: Ichinohe
authors: H
authors: H
authors: Iwatsuki
authors: K
authors: K
authors: Shoumura
mesh_terms: D000818:Animals; D001710:Biotin / Q000031:analogs & derivatives* / Q000493:pharmacokinetics; D003712:Dendrites / Q000378:metabolism / Q000648:ultrastructure; D003911:Dextrans / Q000493:pharmacokinetics; D005456:Fluorescent Dyes / Q000493:pharmacokinetics; D007150:Immunohistochemistry; D020646:Intralaminar Thalamic Nuclei / Q000378:metabolism / Q000648:ultrastructure*; D008297:Male; D008854:Microscopy, Electron; D017072:Neostriatum / Q000378:metabolism / Q000648:ultrastructure*; D009434:Neural Pathways / Q000378:metabolism / Q000648:ultrastructure*; D010320:Parvalbumins / Q000378:metabolism; D017729:Presynaptic Terminals / Q000378:metabolism / Q000648:ultrastructure*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D003911:Dextrans; D005456:Fluorescent Dyes; D010320:Parvalbumins; C076397:biotinylated dextran amine; D001710:Biotin