Phase-sensitive fluorescence lifetime detection in capillary electrophoresis.
A simple and highly sensitive fluorescence lifetime detection method for capillary electrophoresis has been introduced. The detection scheme is based on the integrated phase-sensitive fluorescence intensity. The integrative nature of the method results in high sensitivity of lifetime detection. The limit of detection is 7.8 amol of fluorescein injected, representing a 2 orders of magnitude improvement over the detection limits previously reported in the UV-visible region. Rayleigh scattering, Raman scattering, and background fluorescence can be effectively suppressed by setting the detector out of the phase from the background signal. Fluorescence background can be eliminated whether the fluorescence lifetime of the background is longer or shorter than the solute molecules of interest. The signal-to-noise ratio of measurements is optimized by varying the modulation frequency and the detector phase angle.
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Department of Chemistry, University of Iowa, Iowa City 52242, USA.
Geng
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D000465:Algorithms; D019075:Electrophoresis, Capillary / Q000379:methods*; D005453:Fluorescence; D005456:Fluorescent Dyes; D012542:Scattering, Radiation; D013056:Spectrophotometry, Ultraviolet
D005456:Fluorescent Dyes