Binding of nucleotides to nucleoside diphosphate kinase: a calorimetric study.
The source of affinity for substrates of human nucleoside diphosphate (NDP) kinases is particularly important in that its knowledge could be used to design more effective antiviral nucleoside drugs (e.g., AZT). We carried out a microcalorimetric study of the binding of enzymes from two organisms to various nucleotides. Isothermal titration calorimetry has been used to characterize the binding in terms of Delta G degrees, Delta H degrees and Delta S degrees. Thermodynamic parameters of the interaction of ADP with the hexameric NDP kinase from Dictyostelium discoideum and with the tetrameric enzyme from Myxococcus xanthus, at 20 degrees C, were similar and, in both cases, binding was enthalpy-driven. The interactions of ADP, 2'deoxyADP, GDP, and IDP with the eukaryotic enzyme differed in enthalpic and entropic terms, whereas the Delta G degrees values obtained were similar due to enthalpy--entropy compensation. The binding of the enzyme to nonphysiological nucleotides, such as AMP--PNP, 3'deoxyADP, and 3'-deoxy-3'-amino-ADP, appears to differ in several respects. Crystallography of the protein bound to 3'-deoxy-3'-amino-ADP showed that the drug was in a distorted position, and was unable to interact correctly with active site side chains. The interaction of pyrimidine nucleoside diphosphates with the hexameric enzyme is characterized by a lower affinity than that with purine nucleotides. Titration showed the stoichiometry of the interaction to be abnormal, with 9--12 binding sites/hexamer. The presence of supplementary binding sites might have physiological implications.
Cervoni
L
L
Istituto Pasteur Fondazione Cenci Bolognetti, Università di Roma La Sapienza, 5 P. le Aldo Moro, 00185 Roma, Italy.
Lascu
I
I
Xu
Y
Y
Gonin
P
P
Morr
M
M
Merouani
M
M
Janin
J
J
Giartosio
A
A
D000244:Adenosine Diphosphate / Q000031:analogs & derivatives / Q000737:chemistry; D000255:Adenosine Triphosphate / Q000031:analogs & derivatives / Q000737:chemistry; D000266:Adenylyl Imidodiphosphate / Q000737:chemistry; D000818:Animals; D001665:Binding Sites; D002152:Calorimetry, Differential Scanning / Q000379:methods; D018360:Crystallography, X-Ray; D004023:Dictyostelium / Q000201:enzymology; D006153:Guanosine Diphosphate / Q000737:chemistry; D006801:Humans; D007290:Inosine Diphosphate / Q000737:chemistry; D016941:Myxococcus xanthus / Q000201:enzymology; D009701:Nucleoside-Diphosphate Kinase / Q000737:chemistry*; D011685:Purine Nucleotides / Q000737:chemistry*; D013816:Thermodynamics; D013942:Thymine Nucleotides / Q000737:chemistry; D017186:Titrimetry
D011685:Purine Nucleotides; D013942:Thymine Nucleotides; D006153:Guanosine Diphosphate; D000266:Adenylyl Imidodiphosphate; D000244:Adenosine Diphosphate; D007290:Inosine Diphosphate; D000255:Adenosine Triphosphate; D009701:Nucleoside-Diphosphate Kinase