Antigen-specific T(h)1 cells as direct effectors of Propionibacterium acnes-primed lipopolysaccharide-induced hepatic injury.
T(h)1 cells are cytotoxic effector cells that utilize Fas ligand (FasL) and tumor necrosis factor. The physiological roles of cytotoxic T(h)1 cells are considered to be immunoregulation by eliminating autoreactive lymphocytes or hyper-activated foreign antigen-specific lymphocytes. Their pathological roles, however, remain to be clarified. To investigate whether T(h)1 cells can destroy organs, we generated a Propionibacterium acnes-specific T(h)1 clone from C57BL/6 mice and tested whether the clone could serve as an effector in a P. acnes-primed lipopolysaccharide (LPS)-induced hepatic injury system, one of the septic shock models. B6SMN:C3H-FasL(gld) (B6-gld) mice, which were deficient in functional FasL, were resistant to P. acnes/LPS-induced hepatic shock. The T(h)1 clone rendered B6-gld mice sensitive to the hepatic shock after the i.v. transfer. The hepatic injury in the clone-transferred B6-gld mice, which was evaluated by both biochemical and histological examination, was inhibited by an anti-FasL mAb that we developed. These results suggested that bacterial antigen-specific T(h)1 cells like this clone can participate in organ destruction in vivo as one of the cytotoxic effectors and play a critical role in endotoxin-induced hepatic injury.
Okazaki
T
T
Takasago Research Laboratories, Kaneka Corp., Takasago, Hyogo 676-8688, Japan.
Ozaki
S
S
Nagaoka
T
T
Kozuki
M
M
Sumita
S
S
Tanaka
M
M
Osakada
F
F
Kishimura
M
M
Kakutani
T
T
Nakao
K
K
D019264:Adoptive Transfer; D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D000942:Antigens, Bacterial; D001483:Base Sequence; D002999:Clone Cells; D016207:Cytokines / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D053222:Fas Ligand Protein; D008070:Lipopolysaccharides / Q000633:toxicity*; D008099:Liver / Q000187:drug effects / Q000276:immunology* / Q000293:injuries*; D008297:Male; D008562:Membrane Glycoproteins / Q000172:deficiency / Q000276:immunology; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D011425:Propionibacterium acnes / Q000276:immunology*; D012772:Shock, Septic / Q000276:immunology / Q000473:pathology; D018417:Th1 Cells / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D016207:Cytokines; D017931:DNA Primers; D053222:Fas Ligand Protein; C505658:Fasl protein, mouse; D008070:Lipopolysaccharides; D008562:Membrane Glycoproteins