Comparative expressed-sequence-tag analysis of differential gene expression profiles in BmNPV-infected BmN cells.
To compare the gene expression profiles of uninfected and Bombyx mori nucleopolyhedrovirus (BmNPV)-infected BmN cells, we constructed four cDNA libraries for mock-infected cells, and cells at 2, 6, and 12 h postinfection (h.p.i.). A total of 2645 partial sequences obtained for expressed-sequence-tags (ESTs) from the libraries were categorized using BLAST searches of the public database and the BmNPV genome sequence. The following proportions of BmNPV-derived ESTs were observed: 0.4, 4.5, and 57% at 2, 6, and 12 h.p.i, respectively. Moreover, 31 BmNPV open reading frames (ORFs) were newly identified for transcripts and the baculovirus-repeated ORFs (bro) showed the highest levels of expression in the 12 h.p.i. library. Most of the host genes decreased in number as the infection progressed. However, several, including cytochrome c oxidase 1, increased in the late stages of infection. Two apoptosis-related host genes were also identified.
Okano
K
K
Laboratory of Molecular Entomology and Baculovirology, RIKEN (The Institute of Physical and Chemical Research), Wako, Saitama, Japan. kokano@postman.riken.go.jp
Shimada
T
T
Mita
K
K
Maeda
S
S
D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis / Q000235:genetics; D012831:Bombyx / Q000166:cytology / Q000235:genetics* / Q000821:virology*; D002460:Cell Line; D018076:DNA, Complementary / Q000235:genetics; D019992:Databases as Topic; D003576:Electron Transport Complex IV / Q000235:genetics; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D015967:Gene Expression Regulation, Viral; D005796:Genes / Q000235:genetics; D008969:Molecular Sequence Data; D017924:Nucleopolyhedroviruses / Q000235:genetics* / Q000502:physiology*; D016366:Open Reading Frames / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D013997:Time Factors
D018076:DNA, Complementary; D012333:RNA, Messenger; D003576:Electron Transport Complex IV