title: Effects of flupyrazofos on liver microsomal cytochrome P450 in the male Fischer 344 rat.
abstract: 1. The effects of flupyrazofos on liver microsomal cytochrome P450 were investigated in the male Fischer 344 rat. When rats were treated intraperitoneally with flupyrazofos for 3 consecutive days, the activities of ethoxyresorufin O-deethylase and testosterone 2 beta-hydroxylase were significantly reduced, whereas the activities of pentoxyresorufin beta-depentylase and testosterone 6beta- and 7 alpha-hydroxylases were induced in liver microsomes. 2. Within 24 h after treatment with 50 m kg(-1) flupyrazofos, most enzyme activities were decreased, indicating the interaction of flupyrazofos with cytochrome P450. 3. In Western immunoblotting, cytochrome P4502B1/2 proteins were clearly induced by treatment with flupyrazofos, whereas P4501A1/2 and 2C6 proteins were reduced in liver microsomes. 4. The present results indicate that flupyrazofos modulates the expression of cytochrome P450 in rat.
authors: College of Pharmacy, Wonkwang University, Iksan, Korea.
authors: H S
authors: HS
authors: Lee
authors: H Y
authors: HY
authors: Lee
authors: H K
authors: HK
authors: Gu
authors: S S
authors: SS
authors: Han
authors: C H
authors: CH
authors: Yun
authors: J H
authors: JH
authors: Kim
authors: J A
authors: JA
authors: Kim
authors: E S
authors: ES
authors: Lee
authors: D H
authors: DH
authors: Nam
authors: T C
authors: TC
authors: Jeong
mesh_terms: D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases; D015153:Blotting, Western; D019363:Cytochrome P-450 CYP1A1 / Q000187:drug effects / Q000378:metabolism; D019362:Cytochrome P-450 CYP2B1 / Q000187:drug effects / Q000378:metabolism; D019392:Cytochrome P-450 CYP2E1 / Q000187:drug effects / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000187:drug effects* / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D007306:Insecticides / Q000494:pharmacology*; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism*; D009946:Organothiophosphorus Compounds / Q000494:pharmacology*; D011720:Pyrazoles / Q000494:pharmacology*; D051381:Rats; D011916:Rats, Inbred F344; D013250:Steroid Hydroxylases / Q000187:drug effects / Q000378:metabolism
chemical_list: D007306:Insecticides; D009946:Organothiophosphorus Compounds; D011720:Pyrazoles; C116965:flupyrazofos; D003577:Cytochrome P-450 Enzyme System; D013250:Steroid Hydroxylases; D019392:Cytochrome P-450 CYP2E1; D001189:Aryl Hydrocarbon Hydroxylases; D019363:Cytochrome P-450 CYP1A1; D019362:Cytochrome P-450 CYP2B1; C025619:steroid 16-beta-hydroxylase