Characterization and quantification of mouse mannan-binding lectins (MBL-A and MBL-C) and study of acute phase responses.
Rat monoclonal antibodies (MoAbs) against mouse mannan-binding lectin (MBL)-A and MBL-C were generated and assays for MBL-A and MBL-C were constructed. This allowed for the quantitative analysis of both proteins for the first time. Previously only MBL-A has been quantified using less standardized methods. In a mouse serum pool the concentrations were now determined at 7.5 microg MBL-A and 45 microg MBL-C per ml. On gel permeation chromatography of mouse serum, MBL-A eluted corresponding to a M(r) of 850 kDa whereas the majority of MBL-C eluted corresponding to a Mr of 950 kDa. On sucrose density gradient centrifugation the sedimentation velocities of MBL-A and MBL-C were estimated at 7.3 S and 10.8 S, respectively. The MBL-A and MBL-C levels in 10 laboratory mice strains were compared and found to vary between 4 microg/ml to 12 microg/ml, and 16 microg/ml to 118 microg/ml, respectively. After the induction of acute phase responses by intraperitoneal injection of either casein or lipopolysaccharide (LPS), MBL-A was found to increase approximately two-fold, with a maximum after 32 h, while MBL-C did not increase significantly. In comparison, serum amyloid A component (SAA) peaked at 15 h with an approximate 100-fold increase.
Liu
H
H
Department of Medical Microbiology and Immunology, University of Aarhus, Aarhus, Denmark.
Jensen
L
L
Hansen
S
S
Petersen
S V
SV
Takahashi
K
K
Ezekowitz
A B
AB
Hansen
F D
FD
Jensenius
J C
JC
Thiel
S
S
D000209:Acute-Phase Proteins / Q000096:biosynthesis; D000210:Acute-Phase Reaction / Q000378:metabolism*; D000818:Animals; D015153:Blotting, Western; D002352:Carrier Proteins / Q000032:analysis* / Q000096:biosynthesis / Q000737:chemistry*; D002499:Centrifugation, Density Gradient; D002850:Chromatography, Gel; D037222:Collectins; D005260:Female; D037102:Lectins / Q000032:analysis / Q000096:biosynthesis / Q000737:chemistry; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D051381:Rats; D017208:Rats, Wistar
D000209:Acute-Phase Proteins; D002352:Carrier Proteins; D037222:Collectins; D037102:Lectins