title: Rapid analysis of inositol phosphates.
abstract: Fast and simple analytical methods for the determination of inositol bis- to hexakisphosphates or only inositol hexakisphosphate in foods and feces are presented. The methods are both faster and simpler with regard to analytical detection and sample pretreatment as compared to previously reported methods. The samples are pretreated using extraction and centrifugal ultrafiltration and analyzed using high-performance ion chromatography (HPIC) with gradient or isocratic elution. The analytes are detected using ultraviolet detection after postcolumn reaction. The methods are efficient, highly selective, and appropriate for analyzing inositol phosphates in food and feces samples. The between- and within-day variances were generally below 8 and 5% (relative standard deviation), respectively, for the presented HPIC method with gradient elution.
authors: Department of Food Science, Chalmers University of Technology, S-402 29 Göteborg, Sweden.
authors: N G
authors: NG
authors: Carlsson
authors: E L
authors: EL
authors: Bergman
authors: E
authors: E
authors: Skoglund
authors: K
authors: K
authors: Hasselblad
authors: A S
authors: AS
authors: Sandberg
mesh_terms: D002852:Chromatography, Ion Exchange / Q000379:methods; D005243:Feces / Q000737:chemistry*; D005504:Food Analysis; D007295:Inositol Phosphates / Q000032:analysis*; D012680:Sensitivity and Specificity; D013997:Time Factors
chemical_list: D007295:Inositol Phosphates