Synthesis, conformational studies, and investigations on the estrogen receptor binding of [R/S-1-(2,6-dichloro-4-hydroxyphenyl)ethylenediamine]platinum(II) complexes.
The syntheses, conformational studies, and investigations on the estrogen receptor binding of [R/S-1-(2,6-dichloro-4-hydroxyphenyl)ethylenediamine]platinum(II) complexes (1-PtL2, 2L = leaving groups) are described. A Strecker synthesis using the 2,6-dichloro-4-methoxybenzaldehyde, NaCN, and NH4Cl afforded the cyanoamine 1b, which was subsequently reduced with LiAlH4 to give the R/S-1-(2,6-dichloro-4-methoxyphenyl)ethylenediamine 1a. Ether cleavage with BBr3 yielded R/S-1-(2,6-dichloro-4-hydroxyphenyl)ethylenediamine 1 which was coordinated to platinum(II) by use of K2PtCl4 (1-PtCl2) and K2PtI4 (1-PtI2), respectively. Reaction of 1-PtI2 with Ag2SO4 and coordination of tartronic acid led to the [R/S-1-(2,6-dichloro-4- hydroxyphenyl)ethylenediamine][hydroxymalonato]platinum(II) complex (1-Pt(MalOH)). The spatial structure of 1 and its complexes was evaluated by spectroscopic and molecular modeling methods. In solution the complexes adopt a structure very similar to estradiol. However, the in vitro and in vivo tests for the compounds indicated neither affinity to the estrogen receptor nor estrogenic properties.
Gust
R
R
Institut für Pharmazie, Freien Universität Berlin, Königin-Luise Str. 2 + 4, D-14195 Berlin, Germany.
Lubczyk
V
V
Schmidt
K
K
Shihada
U
U
D002460:Cell Line; D004967:Estrogens / Q000378:metabolism; D006801:Humans; D008156:Luciferases / Q000378:metabolism; D008968:Molecular Conformation; D009944:Organoplatinum Compounds / Q000138:chemical synthesis* / Q000494:pharmacology*; D011960:Receptors, Estrogen / Q000187:drug effects*
D004967:Estrogens; D009944:Organoplatinum Compounds; D011960:Receptors, Estrogen; D008156:Luciferases