Lymphocyte lineages at mucosal effector sites: rat salivary glands.
Development of T cell lineages and the role of the thymus as a source of immature T cells in parotid (PG) and submandibular salivary glands (SMG) were studied in Fischer 344 rats using the Thy-1/CD45RC/RT6 expression model. In addition, the phenotypes of salivary gland lymphocytes were compared with other conventional and extrathymic populations. PG mononuclear cells consisted of T cells (38%), B cells (29%), and NK cells (4%). SMG had 19% T cells, 7% B cells, 37% NK cells, and an unusual population of CD3(-)/RT6(+) cells. In comparison with lymph node (LN), both PG and SMG were enriched in immature (Thy-1(+)) and activated (Thy-1(-)/CD45RC(-)/RT6(-)) T cells. Unchanged percentages of Thy-1(+) T cells in PG and SMG following short-term adult thymectomy indicated that immature salivary gland T cells had an extrathymic source. In contrast, thymectomy eliminated LN recent thymic emigrants. SMG had T cells with characteristics of extrathymic populations, expressing TCRgammadelta(+) (28%), the CD8alphaalpha homodimer (11%), and NKR-P1A (66%). Many SMG T cells expressed integrin alpha(E)beta(7). PG T cells resembled those isolated from LN in respect to TCR and CD8 isoform usage, but were enriched in alpha(E)beta(7)(+) T cells and in NKT cells. Thus, salivary gland mononuclear cells are composed of a variety of subpopulations whose distributions differ between SMG and PG and are distinct from LN. These studies provide a basis for further investigation of regionalization in the mucosal immune network and are relevant to the design of vaccine regimens and intervention during pathological immune processes.
O'Sullivan
N L
NL
Departments of. Anatomy and Cell Biology and Immunology and Microbiology, Wayne State University School of Medicine, Detroit, MI 48201, USA. nosulliv@med.wayne.edu
Skandera
C A
CA
Montgomery
P C
PC
D036002:ADP Ribose Transferases / Q000032:analysis / Q000096:biosynthesis; D000375:Aging / Q000276:immunology; D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface / Q000096:biosynthesis; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D002454:Cell Differentiation / Q000276:immunology; D019070:Cell Lineage / Q000276:immunology; D005260:Female; D016130:Immunophenotyping; D016023:Integrins / Q000096:biosynthesis; D007694:Killer Cells, Natural / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D037181:Lectins, C-Type; D017493:Leukocyte Common Antigens / Q000032:analysis; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000032:analysis / Q000096:biosynthesis; D009061:Mouth Mucosa / Q000166:cytology* / Q000276:immunology* / Q000378:metabolism; D055651:NK Cell Lectin-Like Receptor Subfamily B; D009928:Organ Specificity / Q000276:immunology; D051381:Rats; D011916:Rats, Inbred F344; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000096:biosynthesis; D011971:Receptors, Immunologic / Q000096:biosynthesis; D012469:Salivary Glands / Q000166:cytology* / Q000276:immunology* / Q000378:metabolism; D016176:T-Lymphocyte Subsets / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D018800:Thy-1 Antigens / Q000032:analysis; D013934:Thymectomy
D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface; D016023:Integrins; D037181:Lectins, C-Type; D008562:Membrane Glycoproteins; D055651:NK Cell Lectin-Like Receptor Subfamily B; D016692:Receptors, Antigen, T-Cell, gamma-delta; D011971:Receptors, Immunologic; D018800:Thy-1 Antigens; C070709:integrin alphaEbeta7; D036002:ADP Ribose Transferases; C090163:Art2b protein, rat; D017493:Leukocyte Common Antigens