title: Experimental assessment of the revascularization of acellular human dermis for soft-tissue augmentation.
abstract: The purpose of this experimental study was to determine whether acellular human dermis was capable of complete revascularization in a subcutaneous implantation site with various placement geometries. In young adult rabbit ears, four different sheet and rolled configurations were placed and harvested after 3, 7, 14, and 28 days with silicone rubber microangiographic injections followed by histologic analysis. Revascularization of single-layer acellular human dermis occurred rapidly and was essentially complete by 14 days after surgery. No differences were observed in the ingrowth of vessels regardless of how the basement membrane was oriented. In rolled configurations, vascular ingrowth throughout the implant was slower and had not completely penetrated the grafts by 28 days after surgery at study completion. Vessel ingrowth occurred through the implant surfaces contacting the surrounding soft tissue and along the open seam of the roll. No differences were seen whether the basement membrane was oriented on either the inside or the outside of the roll. Acellular human dermis is capable of significant revascularization of its compact collagen composition in the early postoperative period. In thicker geometries, the rate and completeness of vessel ingrowth are predictably slower. Whether complete revascularization of multilayered or rolled grafts is achieved cannot be determined from this study.
authors: Division of Plastic Surgery, Indiana University School of Medicine, Indianapolis, Indiana, USA. beppley@iupui.edu
authors: B L
authors: BL
authors: Eppley
mesh_terms: D000818:Animals; D004431:Ear, External; D006801:Humans; D018919:Neovascularization, Physiologic; D011817:Rabbits; D019651:Plastic Surgery Procedures; D012867:Skin / Q000098:blood supply / Q000166:cytology; D017768:Skin, Artificial; D014945:Wound Healing