title: Intracameral and lenticular penetration of locally applied stable isotope-labeled vitamin E.
abstract: OBJECTIVE
  To confirm the aqueous humor and lens dynamics of 1% deuterium-labeled alpha-tocopherol acetate (D(3)-VEA) solution.
  
  
  METHODS
  The concentrations of D(3)-VEA and D(3)-alpha-tocopherol (D(3)-VE), derived from D(3)-VEA, in the aqueous humor and lens were measured after continuous instillation of 1% D(3)-VEA into the cul-de-sac of rat eyes for 1 or 3 weeks. D(3)-VEA and D(3)-VE concentrations were determined by gas chromatography/mass selective detector.
  
  
  RESULTS
  In the aqueous humor, the concentrations of D(3)-VEA and D(3)-VE were, respectively, 93.1 and 9.4 ng/mL after 1 week, and, respectively, 498.9 and 21.5 ng/mL after 3 weeks. In the lens, they were, respectively, 15.0 and 9.8 ng/g after 1 week, and 6.1 and 4.8 ng/g after 3 weeks.
  
  
  CONCLUSIONS
  The penetration levels of alpha-tocopherol acetate by eyedrop application were confirmed in the aqueous humor and lens.
authors: Department of Ophthalmology, Kanazawa Medical University, Uchinada, Ishikawa, Japan.
authors: M
authors: M
authors: Nagata
authors: K
authors: K
authors: Kawazu
authors: Y
authors: Y
authors: Midori
authors: M
authors: M
authors: Kojima
authors: E
authors: E
authors: Shirasawa
authors: K
authors: K
authors: Sasaki
mesh_terms: D000287:Administration, Topical; D000818:Animals; D001082:Aqueous Humor / Q000378:metabolism*; D003903:Deuterium; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D007553:Isotope Labeling; D007908:Lens, Crystalline / Q000378:metabolism*; D009883:Ophthalmic Solutions; D051381:Rats; D011914:Rats, Inbred BN; D014018:Tissue Distribution; D014810:Vitamin E / Q000493:pharmacokinetics*
chemical_list: D009883:Ophthalmic Solutions; D014810:Vitamin E; D003903:Deuterium