title: [Effects of low density lipoprotein and oxidized low density lipoprotein on the cytotoxic activity of Asp-hemolysin to murine macrophages].
abstract: We examined the effects of human low density lipoprotein (LDL) and oxidized LDL (Ox-LDL) on the cytotoxic activity of Asp-hemolysin from Aspergillus fumigatus Fresenius-Muramatsu strain to mouse peritoneal macrophages (M phi). The inhibitory effects of LDL and Ox-LDL on the cytotoxic activity of Asp-hemolysin to M phi increased in a dose-dependent manner, and the effect of Ox-LDL was greater than the inhibitory effect of LDL. Furthermore, the binding of Asp-hemolysin to LDL or Ox-LDL was observed by western blot analysis of the culture medium. These results suggest that the inhibition by LDL or Ox-LDL on the cytotoxic activity of Asp-hemolysin to M phi was due to the binding of LDL or Ox-LDL to Asp-hemolysin in the culture medium.
authors: First Department of Hygienic Chemistry, Tohoku Pharmaceutical University, 4-4-1 Komatsushima, Aoba-ku, Sendai 981-8558, Japan.
authors: T
authors: T
authors: Kumagai
authors: T
authors: T
authors: Nagata
authors: Y
authors: Y
authors: Kudo
authors: Y
authors: Y
authors: Fukuchi
authors: K
authors: K
authors: Ebina
authors: K
authors: K
authors: Yokota
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D005656:Fungal Proteins / Q000037:antagonists & inhibitors*; D006460:Hemolysin Proteins; D008077:Lipoproteins, LDL / Q000494:pharmacology*; D017737:Macrophages, Peritoneal / Q000187:drug effects*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains
chemical_list: C089497:Asp-HS protein, Aspergillus fumigatus; D005656:Fungal Proteins; D006460:Hemolysin Proteins; D008077:Lipoproteins, LDL; C099252:oxidized low density lipoprotein