title: Neovascularization in experimental retinal venous obstruction in rabbits.
abstract: OBJECTIVE
  To investigate the later pathohistological changes in experimental retinal venous obstruction in rabbits.
  
  
  METHODS
  Experimental retinal venous obstruction was produced in retinal blood vessels of rabbits by trans-adventitial dropping of thrombin. Fundus observation was performed 3 months and 1 year thereafter. Retinal histological examinations were performed by fluorescein microscopy, light microscopy, and transmission electron microscopy.
  
  
  RESULTS
  Vessels with a rete mirabile and arteriovenous anastomosis were observed after 3 months. On a flat gelatin-fluorescein preparation, regions with no perfusion, thought to be areas of vascular occlusion in the periphery, were observed extensively. Clear leakage of gelatin-added fluorescein was noted from vessels in the periphery, and minute neovascularization with a rete mirabile from these vessels was confirmed. Newly formed retinal vessels were also observed by transmission electron microscopy. The endothelial cells of these newly formed vessels had a large nucleus, a number of ribosomes, and thin basement membrane. Proliferative changes included glial cells that had penetrated into the basement membrane of ghost vessels.
  
  
  CONCLUSIONS
  Our long-term observations confirmed that proliferative changes and neovascularization occurred in this model of retinal venous obstruction.
authors: Department of Ophthalmology, Hirosaki University School of Medicine, Hirosaki, Aomori, Japan.
authors: M
authors: M
authors: Tamura
mesh_terms: D000818:Animals; D001485:Basement Membrane / Q000648:ultrastructure; D002199:Capillary Permeability; D002467:Cell Nucleus / Q000648:ultrastructure; D004730:Endothelium, Vascular / Q000648:ultrastructure; D005451:Fluorescein Angiography; D005654:Fundus Oculi; D023421:Models, Animal; D011817:Rabbits; D015861:Retinal Neovascularization / Q000175:diagnosis / Q000209:etiology*; D012169:Retinal Vein / Q000187:drug effects / Q000473:pathology; D012170:Retinal Vein Occlusion / Q000139:chemically induced / Q000150:complications*; D012270:Ribosomes / Q000648:ultrastructure; D013917:Thrombin / Q000633:toxicity
chemical_list: D013917:Thrombin