Effects of cell banking manipulations on ex vivo amplification of umbilical cord blood.
The small volume of placental/umbilical cord blood (PUCB) collectable restricts the use of these stem cells to pediatric transplantation. To extend the use of PUCB to adult recipients, many laboratories are investigating the feasibility of ex vivo PUCB expansion. The present study analyses the effects that PUCB banking cell manipulations (cell sedimentation, cryopreservation and thawing, mononuclear and CD34+ cell isolation) have on the number, viability and ex vivo expansion potential of PUCB cells. The results presented indicate the necessity of an open discussion on whether procedures used for handling the cells in PUCB banks can be extrapolated or not as such to the clinical use of ex vivo expanded PUCB.
Forte
L
L
Dipartimento di Ematologia, Università degli Studi Tor Vergata, Rome, Italy.
Migliaccio
G
G
Sanchez
M
M
Migliaccio
A R
AR
Passarelli
A M
AM
Amadori
S
S
D000328:Adult; D018952:Antigens, CD34 / Q000032:analysis; D000094902:Blood Banking / Q000379:methods*; D001772:Blood Cell Count; D001793:Blood Preservation; D001800:Blood Specimen Collection; D001835:Body Weight; D002470:Cell Survival; D002648:Child; D003114:Colony-Forming Units Assay; D015925:Cryopreservation; D003451:Cryoprotective Agents / Q000494:pharmacology; D005312:Fetal Blood / Q000166:cytology*; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000187:drug effects; D006801:Humans; D018189:Immunomagnetic Separation; D007231:Infant, Newborn
D018952:Antigens, CD34; D003451:Cryoprotective Agents