title: Flagellar radial spoke protein 3 is an A-kinase anchoring protein (AKAP).
abstract: Previous physiological and pharmacological experiments have demonstrated that the Chlamydomonas flagellar axoneme contains a cAMP-dependent protein kinase (PKA) that regulates axonemal motility and dynein activity. However, the mechanism for anchoring PKA in the axoneme is unknown. Here we test the hypothesis that the axoneme contains an A-kinase anchoring protein (AKAP). By performing RII blot overlays on motility mutants defective for specific axonemal structures, two axonemal AKAPs have been identified: a 240-kD AKAP associated with the central pair apparatus, and a 97-kD AKAP located in the radial spoke stalk. Based on a detailed analysis, we have shown that AKAP97 is radial spoke protein 3 (RSP3). By expressing truncated forms of RSP3, we have localized the RII-binding domain to a region between amino acids 144-180. Amino acids 161-180 are homologous with the RII-binding domains of other AKAPs and are predicted to form an amphipathic helix. Amino acid substitution of the central residues of this region (L to P or VL to AA) results in the complete loss of RII binding. RSP3 is located near the inner arm dyneins, where an anchored PKA would be in direct position to modify dynein activity and regulate flagellar motility.
authors: Department of Cell Biology, Emory University School of Medicine, Atlanta, Georgia 30322, USA.
authors: A R
authors: AR
authors: Gaillard
authors: D R
authors: DR
authors: Diener
authors: J L
authors: JL
authors: Rosenbaum
authors: W S
authors: WS
authors: Sale
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D002465:Cell Movement / Q000502:physiology; D002696:Chlamydomonas / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism*; D005407:Flagella / Q000201:enzymology / Q000378:metabolism*; D008954:Models, Biological; D008969:Molecular Sequence Data; D010940:Plant Proteins; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015800:Protozoan Proteins; D016415:Sequence Alignment
chemical_list: D010940:Plant Proteins; D011506:Proteins; D015800:Protozoan Proteins; C060324:radial spoke protein, Chlamydomonas; D017868:Cyclic AMP-Dependent Protein Kinases