The results of an mtDNA study of 1,200 inhabitants of a German village in comparison to other Caucasian databases and its relevance for forensic casework.
Mitochondrial DNA control region sequences were determined in 1,200 male volunteers from one village area of Lower Saxony for the hypervariable region 1 (HV1). The 154 variable positions found resulted in 460 different haplotypes with a haplotype diversity value of 0.98165. The number of different haplotypes showed a nearly linear increase with the number of individuals typed. The haplotype diversity approached saturation level at a value of approximately 0.981 after typing 400 individuals. Furthermore, the number of different haplotypes and the haplotype diversity were calculated for four short amplicons of HV1 in order to establish the most variable section with a high efficiency for forensic casework.
Pfeiffer
H
H
Institute of Legal Medicine, University of Münster, Germany. pfeiffh@uni-muenster.de
Forster
P
P
Ortmann
C
C
Brinkmann
B
B
D000293:Adolescent; D000328:Adult; D017931:DNA Primers; D004272:DNA, Mitochondrial / Q000032:analysis / Q000235:genetics*; D016208:Databases, Factual; D018732:Forensic Anthropology / Q000379:methods; D005554:Forensic Medicine / Q000379:methods*; D014644:Genetic Variation; D005858:Germany; D006239:Haplotypes; D006801:Humans; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics*; D012680:Sensitivity and Specificity; D044465:White People / Q000235:genetics*
D017931:DNA Primers; D004272:DNA, Mitochondrial