T-cell factor-1 expression during human natural killer cell development and in circulating CD56(+) bright natural killer cells.
Transcription factors are essential to govern differentiation along the lymphoid lineage from uncommitted hematopoietic stem cells. Although many of these transcription factors have putative roles based on murine knockout experiments, their function in human lymphoid development is less known and was studied further. Transcription factor expression in fresh and cultured adult human bone marrow and umbilical cord blood progenitors was evaluated. We found that fresh CD34(+)Lin(-) cells that are human leukocyte antigen (HLA)-DR(-) or CD38(-) constitutively express GATA-3 but not T-cell factor-1 (TCF-1) or Id-3. Culture with the murine fetal liver cell line AFT024 and defined cytokines was capable of inducing TCF-1 mRNA. However, no T-cell receptor gene rearrangement was identified in cultured progeny. Id-3, a basic helix loop helix factor with dominant negative function for T-cell differentiation transcription factors, also was upregulated and may explain unsuccessful T-cell maturation. To better understand the developmental link between natural killer (NK) cells derived from progenitors, we studied NK cell subsets circulating in blood. CD56(+bright), but not CD56(+dim), NK cells constitutively express TCF-1 by reverse transcriptase polymerase chain reaction and Western blot analysis. The TCF-1 isoform found in CD56(+bright) cells, which express lectin but not immunoglobulin class I recognizing inhibitory receptors, was identical to that induced in NK cell differentiation culture and was distinctly different from isoforms in T cells. These results suggest that TCF-1 does not target human killer immunoglobulin receptor genes, TCF-1 is uniquely expressed in circulating CD56(+bright) NK cells, and specific TCF-1 isoforms may play an important role in regulating NK differentiation from a common NK/T-cell progenitor.
Toor
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AA
Department of Medicine, University of Minnesota Cancer Center, Harvard Street at East River Road, Minneapolis, MN 55455, USA.
Lund
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TC
Miller
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JS
D036541:ADP-ribosyl Cyclase; D051997:ADP-ribosyl Cyclase 1; D000818:Animals; D015703:Antigens, CD; D018952:Antigens, CD34 / Q000032:analysis; D000943:Antigens, Differentiation / Q000032:analysis; D015153:Blotting, Western; D019002:CD56 Antigen / Q000032:analysis*; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000235:genetics*; D015870:Gene Expression; D006684:HLA-DR Antigens / Q000032:analysis; D018257:Helix-Loop-Helix Motifs; D006412:Hematopoietic Stem Cells / Q000276:immunology / Q000502:physiology; D051538:Hepatocyte Nuclear Factor 1-alpha; D006801:Humans; D051796:Inhibitor of Differentiation Proteins; D007694:Killer Cells, Natural / Q000276:immunology / Q000502:physiology*; D051821:Lymphoid Enhancer-Binding Factor 1; D008562:Membrane Glycoproteins; D051379:Mice; D009244:NAD+ Nucleosidase / Q000032:analysis; D009363:Neoplasm Proteins; D012333:RNA, Messenger / Q000032:analysis; D011975:Receptors, Mitogen / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051822:T Cell Transcription Factor 1; D014157:Transcription Factors / Q000032:analysis / Q000235:genetics*
D015703:Antigens, CD; D018952:Antigens, CD34; D000943:Antigens, Differentiation; D019002:CD56 Antigen; D004268:DNA-Binding Proteins; D006684:HLA-DR Antigens; D051538:Hepatocyte Nuclear Factor 1-alpha; C496488:Hnf1a protein, mouse; D051796:Inhibitor of Differentiation Proteins; D051821:Lymphoid Enhancer-Binding Factor 1; D008562:Membrane Glycoproteins; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D011975:Receptors, Mitogen; D051822:T Cell Transcription Factor 1; C497576:TCF7 protein, human; D014157:Transcription Factors; C075640:ID3 protein, human; D036541:ADP-ribosyl Cyclase; C498154:CD38 protein, human; C498155:Cd38 protein, mouse; D009244:NAD+ Nucleosidase; D051997:ADP-ribosyl Cyclase 1