Tetranectin-like protein in vertebrate serum: a comparative immunochemical analysis.
The glycoprotein tetranectin (TN) found in human serum is a 90-kDa homotrimeric C-type lectin binding Ca2+, heparin and plasminogen kringle 4. TN is suggested as being implicated in tissue remodelling. The antigenic reactivity of putative TN was examined in serum from 14 different animal species using three sandwich enzyme immunoassays for human TN. Crab-eating macaque serum showed the strongest reaction, followed by horse and cat. Serum from cow, goat, pig, mouse and chicken reacted weakly, while dog, trout, and the amphibian and the reptile species did not react. The TN-like protein from macaque, horse and cat serum bound heparin and showed the same dependence on Ca2+ for interaction with the monoclonal antibodies as human TN. Gel filtration of sera from the three animal species showed that the TN-like protein eluted as single peaks with a M(r) of 70-90 kDa. Western blotting of horse and cat TN-like protein electrophoresed under reducing conditions showed that the antibodies against human TN reacted with a single band with an approximate M(r) of 30 kDa, indicating that the TN-like protein is also a homotrimer. Horse and cat TN-like protein interacted with human kringle 4-sepharose. Most likely, the reacting protein represents crab-eating macaque, horse and cat homologues of human TN.
Thougaard
A V
AV
Department of Clinical Biochemistry, Statens Serum Institut, 5 Artillerivej, DK-2300 S, Copenhagen, Denmark.
Jaliashvili
I
I
Christiansen
M
M
D000818:Animals; D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor / Q000276:immunology; D001798:Blood Proteins / Q000276:immunology* / Q000378:metabolism; D002118:Calcium / Q000494:pharmacology; D002415:Cats; D003429:Cross Reactions / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006736:Horses; D006801:Humans; D007150:Immunohistochemistry; D018082:Kringles; D037102:Lectins / Q000276:immunology; D037181:Lectins, C-Type; D008252:Macaca fascicularis; D010958:Plasminogen / Q000378:metabolism; D011485:Protein Binding; D013045:Species Specificity; D014714:Vertebrates / Q000097:blood*
D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D001798:Blood Proteins; D037102:Lectins; D037181:Lectins, C-Type; C048648:tetranectin; D010958:Plasminogen; D002118:Calcium