title: Effects of FIS overexpression on cell growth, rRNA synthesis, and ribosome content in Escherichia coli.
abstract: The Escherichia coli DNA binding protein FIS is a transcriptional modulator involved in the regulation of many cellular processes, including the activation of rRNA synthesis. High-level overproduction of FIS in early, mid, or late log cultures resulted in growth-phase- and media-specific variations in cell growth, rRNA synthesis, and ribosome content. FIS overproduction caused a pronounced increase in rRNA synthesis for late-exponential cultures but a substantial reduction in cell growth and ribosome content. The addition of simple sugars such as glucose or fructose reversed these phenomena, consistent with the functional role of FIS in carbon metabolism.
authors: Department of Chemical and Environmental Engineering, University of California, Riverside, California 92521, USA.
authors: R
authors: R
authors: Richins
authors: W
authors: W
authors: Chen
mesh_terms: D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics* / Q000302:isolation & purification; D002455:Cell Division / Q000235:genetics*; D017931:DNA Primers; D004926:Escherichia coli / Q000166:cytology* / Q000235:genetics / Q000648:ultrastructure; D029968:Escherichia coli Proteins; D035021:Factor For Inversion Stimulation Protein; D034982:Integration Host Factors; D012335:RNA, Ribosomal / Q000096:biosynthesis*; D012270:Ribosomes
chemical_list: D002352:Carrier Proteins; D017931:DNA Primers; D029968:Escherichia coli Proteins; D035021:Factor For Inversion Stimulation Protein; D034982:Integration Host Factors; D012335:RNA, Ribosomal; C440502:integration host factor, E coli