A highly convergent synthesis of a complex oligosaccharide derived from group B type III Streptococcus.
An efficient synthesis of a heptasaccharide derived from group B type III Streptococcus carrying an artificial spacer (1) is described. Rapid assembly of a protected heptasaccharide (16a) is accomplished from readily available building blocks 2-5 without a single protecting group manipulation between glycosylation steps. The synthetic strategy may be applied to the assembly of other branched complex oligosaccharides. The deprotected heptasaccharide 1 was coupled to a poly[N-(acryloyloxy)succinimide, and the resulting material will be used for the development of an ELISA assay to detect antibodies against GBS, type III in pregnant women.
Demchenko
A V
AV
Complex Carbohydrate Research Center, University of Georgia, 220 Riverbend Road, Athens, GA 30602, USA.
Boons
G J
GJ
D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000737:chemistry; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000138:chemical synthesis*; D011108:Polymers / Q000138:chemical synthesis; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000276:immunology; D011247:Pregnancy; D013292:Streptococcus agalactiae / Q000737:chemistry* / Q000276:immunology
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D009844:Oligosaccharides; D011108:Polymers; D011135:Polysaccharides, Bacterial