Detection and multigenic characterization of a novel gammaherpesvirus in goats.
Evidence for the existence of a caprine gammaherpesvirus was obtained by analysis of peripheral blood leucocytes of goats with PCR assays that target the herpesvirus genes encoding the glycoprotein B (gB), the DNA polymerase (DPOL) and the terminase (TERM) with degenerate and deoxyinosine-substituted primers. A contiguous 3.6 kbp sequence extending from the gB to the DPOL gene was then determined with specific primers. All sequences (gB, DPOL and TERM) showed a close relationship with the corresponding genes of the Gammaherpesvirinae. Alignment of amino acid sequences revealed a particularly high percentage of identity with the ovine herpesvirus type 2 (>83%), followed by the alcelaphine herpesvirus 1 (>76%) and the bovine lymphotropic herpesvirus (>61%). Phylogenetic analyses confirmed these relationships. The putative novel goat herpesvirus from which these sequences originate was tentatively designated caprine herpesvirus 2. This virus is the first gammaherpesvirus recognized in goats.
Chmielewicz
B
B
Robert Koch-Institut, Nordufer 20, 13353, Berlin, Germany.
Goltz
M
M
Ehlers
B
B
D000818:Animals; D001482:Base Composition; D002417:Cattle; D017931:DNA Primers; D004259:DNA-Directed DNA Polymerase / Q000235:genetics; D004706:Endodeoxyribonucleases / Q000235:genetics; D018142:Gammaherpesvirinae / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D016679:Genome, Viral; D006023:Glycoproteins / Q000235:genetics; D006041:Goats / Q000097:blood / Q000821:virology*; D007963:Leukocytes, Mononuclear / Q000821:virology; D008969:Molecular Sequence Data; D010802:Phylogeny; D012756:Sheep; D014764:Viral Proteins / Q000235:genetics*
D017931:DNA Primers; D006023:Glycoproteins; D014764:Viral Proteins; D004259:DNA-Directed DNA Polymerase; D004706:Endodeoxyribonucleases; C030894:terminase