Use of methanolysis for the determination of total ellagic and gallic acid contents of wood and food products.
Anhydrous methanolic HCl has been found to be an excellent reagent for releasing ellagic acid and gallic acid (as methyl gallate) from biomass substrates. Optimization of both the reaction conditions and the gradient HPLC analysis has led to the development of a new protocol. The method provides ellagic acid yields significantly higher than those obtained previously, indicating total ellagic acid contents of several substrates have previously been underestimated.
Lei
Z
Z
Department of Wood Science and Forest Products, Fralin Biotechnology Center, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061-0346, USA.
Jervis
J
J
Helm
R F
RF
D000434:Alcoholic Beverages / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004610:Ellagic Acid / Q000032:analysis*; D005504:Food Analysis / Q000379:methods*; D005638:Fruit / Q000737:chemistry; D005707:Gallic Acid / Q000032:analysis*; D006851:Hydrochloric Acid; D007202:Indicators and Reagents; D000432:Methanol; D014197:Trees / Q000737:chemistry; D014934:Wood
D007202:Indicators and Reagents; D004610:Ellagic Acid; D005707:Gallic Acid; D006851:Hydrochloric Acid; D000432:Methanol