Expression and sequence analysis of the Blumeria graminis mitogen-activated protein kinase genes, mpk1 and mpk2.
Mitogen-activated protein (MAP) kinases represent a group of serine/threonine kinases which play a pivotal role in signal transduction processes in eukaryotic cells. Using degenerate PCR primer design based on published and aligned MAP kinase sequences we have cloned and characterised two MAP kinase genes from the barley powdery mildew fungus, Blumeria graminis. We have utilised 'step down' PCR to attain the full length mildew genomic clones. The single-copy genes, named mpk1 and mpk2, encode putative proteins of 356 and 410 amino acids and carry three and four introns, respectively. Expression studies, using RT-PCR, reveal a differing pattern of tissue gene expression with mpk1 and mpk2 during germling morphogenesis and this is compared with the constitutive expression of the 'control' beta-tubulin gene.
Zhang
Z
Z
Department of Plant Sciences, University of Oxford, South Parks Road, OX1 3RB, Oxford, UK.
Gurr
S J
SJ
D000595:Amino Acid Sequence; D001203:Ascomycota / Q000201:enzymology / Q000235:genetics*; D015139:Blotting, Southern; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D015971:Gene Expression Regulation, Enzymologic; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal / Q000235:genetics*; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic
D004271:DNA, Fungal; D020928:Mitogen-Activated Protein Kinases