Pressure monitoring of continuous-flow solid-phase peptide synthesis.
We describe the noninvasive real-time pressure monitoring of Boc- and Fmoc-based peptide synthesis. Pressure was measured using a resistance strain gauge attached to the inlet of a continuous-flow reactor of variable volume. In the assembly of the 'difficult' polyalanine sequence, it was shown that pressure monitoring can reveal structural variations of the peptide-resin, e.g. the onset, development and termination of aggregation. This method provided washing minimization that favored substantial saving of solvents. The obtained results demonstrated the advantage of pressure monitoring over swellographic monitoring.
Baru
M B
MB
Branch of Shemyakin and Ovchinikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Puschino. synchro@fibkh.serpukhov.su
Mustaeva
L G
LG
Vagenina
I V
IV
Gorbunova
E Y
EY
Cherskii
V V
VV
D001671:Biochemistry / Q000295:instrumentation / Q000379:methods*; D010455:Peptides / Q000138:chemical synthesis*; D011312:Pressure; D012997:Solvents
D010455:Peptides; D012997:Solvents; C019529:polyalanine