Effect of anticoagulants and storage temperatures on stability of plasma and serum hormones.
OBJECTIVE
To determine the effect of different anticoagulants and storage conditions on the stability of hormones in plasma and serum.


METHODS
Human blood samples were collected from volunteers into EDTA, lithium heparin, sodium fluoride/potassium oxalate, or tubes without anticoagulant, plasma and serum left at -20 degrees C, 4 degrees C or 30 degrees C for 24 and 120 hours then assayed for ACTH, aldosterone, alpha-subunit, AVP, CRH, C-peptide, estradiol, FSH, glucagon, GH, IGF-1, IGFBP-3, insulin, leptin, LH, PPP, PTH, prolactin and VIP, or at room temperature for 0 to 72 hours (BNP, NT-BNP)(n = 6 per condition).


RESULTS
The anticoagulant altered the measured concentrations for 9 hormones when compared to EDTA. All hormones except ACTH were stable for > 120 hours in EDTA or fluoride at 4 degrees C, but only 13 hormones were stable in all anticoagulants. At 30 degrees C, 8 hormones were stable for > 120 hours in EDTA, and 3 hormones in all anticoagulants. BNP and NT-BNP were stable for < 24 hours when stored in EDTA or heparin at room temperature.


CONCLUSIONS
Storage of samples in EDTA plasma at 4 degrees C is suitable for most hormones (except ACTH) for up to 120 hours.
Evans
M J
MJ
Endolab, Department of Endocrinology, Christchurch Hospital, PO Box 4710, Christchurch, New Zealand. margaret.evans@chmeds.ac.nz
Livesey
J H
JH
Ellis
M J
MJ
Yandle
T G
TG
D000925:Anticoagulants / Q000494:pharmacology*; D004492:Edetic Acid / Q000494:pharmacology; D006728:Hormones / Q000097:blood*; D006801:Humans; D010070:Oxalates / Q000494:pharmacology; D010949:Plasma / Q000737:chemistry*; D012969:Sodium Fluoride / Q000494:pharmacology; D013048:Specimen Handling / Q000379:methods*; D013696:Temperature; D013997:Time Factors
D000925:Anticoagulants; D006728:Hormones; D010070:Oxalates; D012969:Sodium Fluoride; D004492:Edetic Acid