title: Identification of anchovy (Engraulis encrasicholus L.) and gilt sardine (Sardinella aurita) by polymerase chain reaction, sequence of their mitochondrial cytochrome b gene, and restriction analysis of polymerase chain reaction products in semipreserves.
abstract: A method of authenticating anchovy (Engraulis encrasicholus L.) and gilt sardine (Sardinella aurita) semipreserves (salt-cured and fillets in oil) has been developed by polymerase chain reaction (PCR) followed by sequence and restriction site analysis. The amplification of a fragment of the cytochrome b gene by universal primers produced a 376 base pairs (bp) fragment in all samples analyzed. Digestion of PCR products with XhoI, TaqI, AluI, and HinfI endonucleases yielded species-specific profiles distinguishing anchovy from gilt sardine. Therefore, the restriction length fragment polymorphism (RLFP) technique can be used to determine the species identity of anchovy and gilt sardine in semipreserves.
authors: Dipartimento di Sanità, Patologia, Farmaco-Tossicologia e Benessere degli Animali, Sezione di Ispezione degli Alimenti, Facoltà di Medicina Veterinaria, Università degli Studi di Bari, 70010 Valenzano (Bari), Italy.
authors: P
authors: P
authors: Sebastio
authors: P
authors: P
authors: Zanelli
authors: T M
authors: TM
authors: Neri
mesh_terms: D000818:Animals; D001483:Base Sequence; D003573:Cytochrome b Group / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000032:analysis* / Q000235:genetics*; D005399:Fishes / Q000145:classification* / Q000235:genetics; D005519:Food Preservation; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping / Q000379:methods; D016415:Sequence Alignment
chemical_list: D003573:Cytochrome b Group; D004272:DNA, Mitochondrial