Effects of Mucuna pruriens extract on activation of prothrombin by Echis carinatus venom.
Mucuna pruriens (L.) DC has long been used as a medicinal plant by traditional healers. The validity of the claims made for this plant has also been tested scientifically. Some of its properties are probably linked to high concentrations of dopa since it is useful in the treatment of Parkinson's disease. The antisnake properties of an extract of Mucuna pruriens' seeds (MP101UJ) in vivo were recently demonstrated and one is now investigating its biochemical mechanism. Echis carinatus venom (EV) contains a mixture of proteins that affect the coagulative cascade, causing severe bleeding and haemorrhage. Here the effect of an extract of MP101UJ in prothrombin activation by EV in vitro by clotting and chromogenic assay is studied. An increase in procoagulant activity was found. This could explain the protective effect in vivo.
Guerranti
R
R
Institute of Biochemistry and Enzymology, University of Siena, Via A. Moro, 53100, Siena, Italy. guerranti@unisi.it
Aguiyi
J C
JC
Errico
E
E
Pagani
R
R
Marinello
E
E
D000818:Animals; D000997:Antivenins / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D051379:Mice; D008813:Mice, Inbred ICR; D010936:Plant Extracts / Q000494:pharmacology*; D011516:Prothrombin / Q000378:metabolism*; D019685:Rosales / Q000737:chemistry*; D014757:Viper Venoms / Q000633:toxicity*
D000997:Antivenins; D010936:Plant Extracts; D014757:Viper Venoms; D011516:Prothrombin