Oxidative stress increases MICA and MICB gene expression in the human colon carcinoma cell line (CaCo-2).
The human major histocompatibility complex class I chain-related A gene (MICA) and the MICB gene are newly identified members of the major histocompatibility complex class I chain-related gene family. We demonstrate here that oxidative stress, induced by H(2)O(2), promoted MICA (2.2-fold) and MICB (3.8-fold) gene expression using the human colon carcinoma cell line (CaCo-2) and semi-quantitative RT-PCR.
Yamamoto
K
K
Department of Clinical Laboratory Medicine, Shiga University of Medical Science, Otsu, Japan. yamak@belle.shiga-med.ac.jp
Fujiyama
Y
Y
Andoh
A
A
Bamba
T
T
Okabe
H
H
D018938:Caco-2 Cells / Q000378:metabolism*; D005786:Gene Expression Regulation; D015395:Histocompatibility Antigens Class I / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D006861:Hydrogen Peroxide; D008565:Membrane Proteins / Q000378:metabolism*; D018384:Oxidative Stress; D020133:Reverse Transcriptase Polymerase Chain Reaction
D015395:Histocompatibility Antigens Class I; C099187:MHC class I-related chain A; C098080:MICB antigen; D008565:Membrane Proteins; D006861:Hydrogen Peroxide