Electrophysiological analysis of responses of adult females of Brugia pahangi to some chemicals.
Electrophysiological techniques were used to obtain recordings of extracellular electrical activity from the anterior end of live adult females of Brugia pahangi. Stimulation with 100 mM, 10 mM and 1 mM acetylcholine resulted in an increase in spike activity which was concentration dependent, whereas stimulation with phosphate-buffered saline and 0.1 mM acetylcholine gave no increase in activity. The delay in response was not concentration dependent. The action of possible host cues was investigated. Stimulation with heat-inactivated foetal calf serum (IFCS) and 10 mM glutathione gave an increase in spike activity but exposure to 5 mg/dl haemoglobin elicited no response. The response to IFCS was found to be suppressed completely by pre-incubation for 30 min in ivermectin.
Rolfe
R N
RN
Entomology and Nematology Department, IACR-Rothamsted, Harpenden, Herts, UK.
Barrett
J
J
Perry
R N
RN
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D000981:Antiprotozoal Agents / Q000494:pharmacology; D017179:Brugia pahangi / Q000187:drug effects* / Q000502:physiology*; D018678:Cholinergic Agents / Q000494:pharmacology; D004594:Electrophysiology; D005260:Female; D005978:Glutathione / Q000494:pharmacology; D006358:Hot Temperature; D007559:Ivermectin / Q000494:pharmacology
D000981:Antiprotozoal Agents; D018678:Cholinergic Agents; D007559:Ivermectin; D005978:Glutathione; D000109:Acetylcholine