Plasticity of monkey triceps muscle fibers in microgravity conditions.
We examined the changes in functional properties of triceps brachii skinned fibers from monkeys flown aboard the BION 11 satellite for 14 days and after ground-based arm immobilization. The composition of myosin heavy chain (MHC) isoforms allowed the identification of pure fibers containing type I (slow) or type IIa (fast) MHC isoforms or hybrid fibers coexpressing predominantly slow (hybrid slow; HS) or fast (hybrid fast) MHC isoforms. The ratio of HS fibers to the whole slow population was higher after flight (28%) than in the control population (7%), and the number of fast fibers was increased (up to 86% in flight vs. 12% in control). Diameters and maximal tensions of slow fibers were decreased after flight. The tension-pCa curves of slow and fast fibers were modified, with a decrease in pCa threshold and an increase in steepness. The proper effect of microgravity was distinguishable from that of immobilization, which induced less marked slow-to-fast transitions (only 59% of fast fibers) and changed the tension-pCa relationships.
Kischel
P
P
Laboratoire de Plasticité Neuromusculaire, Université des Sciences et Technologies de Lille 1, F-59655 Villeneuve d'Ascq Cedex, France.
Stevens
L
L
Montel
V
V
Picquet
F
F
Mounier
Y
Y
D000818:Animals; D007103:Immobilization; D008253:Macaca mulatta; D009119:Muscle Contraction / Q000502:physiology; D018656:Muscle Fibers, Fast-Twitch / Q000737:chemistry / Q000166:cytology / Q000502:physiology*; D018657:Muscle Fibers, Slow-Twitch / Q000737:chemistry / Q000166:cytology / Q000502:physiology*; D018482:Muscle, Skeletal / Q000737:chemistry / Q000166:cytology / Q000502:physiology*; D018995:Myosin Heavy Chains / Q000032:analysis*; D020033:Protein Isoforms / Q000032:analysis; D012016:Reference Values; D013026:Space Flight; D014893:Weightlessness
D020033:Protein Isoforms; D018995:Myosin Heavy Chains