Demonstration of the Rb1 lipopolysaccharide core structure in Salmonella strains with the monoclonal antibody M105.
The lipopolysaccharide (LPS) from 42 strains representing 19 Salmonella serogroups was differentiated into characteristic ladder-like profiles by SDS-PAGE analysis. The core-specific antibody M105 (Ra, Rb1 and Rb2) was used in an immunoblot assay of SDS-PAGE-separated LPS molecules. The M105 antibody bound to the R-type LPS of 18 of the 20 Salmonella strains tested. The results demonstrate that S. enterica serotype Godesberg, S. Adelaide (one of two strains), S. Milwaukee, S. Niarembe, S. Bere and S. Arizonae (serogroup 63) have an atypical LPS core structure which is Rb1 type.
Mansfield
Lucielle P
LP
Department of Life Sciences, Nottingham Trent University, Clifton Lane, Nottingham NG11 8NS.
Forsythe
S J
SJ
Department of Life Sciences, Nottingham Trent University, Clifton Lane, Nottingham NG11 8NS.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D015151:Immunoblotting; D008070:Lipopolysaccharides / Q000737:chemistry / Q000276:immunology*; D012475:Salmonella / Q000737:chemistry / Q000145:classification* / Q000276:immunology; D012703:Serotyping
D000911:Antibodies, Monoclonal; D008070:Lipopolysaccharides