Microglial activation, emergence of ED1-expressing cells and clusterin upregulation in the aging rat CNS, with special reference to the spinal cord.
With advancing age, the incidence of neuronal atrophy and dystrophy increases and, in parallel, behavioural sensorimotor impairment becomes overt. Activated microglia has been implicated in cytotoxic and inflammatory processes in neurodegenerative diseases as well as during aging. Here we have used immunohistochemistry and in situ hybridization to examine the expression of OX42, ED1, ED2, GFAP and clusterin in CNS of young adult and behaviourally tested aged rats (30-month-old), to study the occurrence of activated microglia/ED1 positive macrophages in senescence and to what extent this correlates with astrogliosis and signs of sensorimotor impairment among the individuals. The results show a massive region-specific increase in activated microglia and ED1 expressing cell profiles in aged rats. The infiltration was most prominent in the spinal cord dorsal columns, including their sensory relay nuclei, and the outer portions of the lateral and ventral columns. At such sites the occurrence of macrophages coincided with increased levels of GFAP and positive correlations were evident between the labeling for, on the one hand, OX42 and, on the other, GFAP and ED1. Also, the ventral and dorsal roots were heavily infiltrated by ED1 positive cells. The signs of gliosis were most pronounced among aged rats with advanced sensorimotor impairment. In contrast, the grey matter of aged rats showed very few activated microglia/ED1 labeled cells despite signs of focal astrogliosis. ED2 expression was confined to perivascular cells and leptominges with a similar labeling pattern in young and aged rats. In aged rats increased expression of clusterin was observed in GFAP-immunoreactive profiles of the white matter only. It is suggested that this increase may reflect a response to degenerative/inflammatory processes.
Kullberg
S
S
Department of Neuroscience, Karolinska Institutet, Doktorsringen 17, S-171 77, Stockholm, Sweden.
Aldskogius
H
H
Ulfhake
B
B
D000375:Aging / Q000378:metabolism* / Q000502:physiology; D000818:Animals; D001921:Brain / Q000166:cytology / Q000378:metabolism; D001933:Brain Stem / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D002490:Central Nervous System / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D051152:Clusterin; D004476:Ectodermal Dysplasia / Q000378:metabolism; D053331:Ectodysplasins; D005260:Female; D006023:Glycoproteins / Q000032:analysis / Q000096:biosynthesis*; D008297:Male; D008565:Membrane Proteins / Q000032:analysis / Q000096:biosynthesis*; D017628:Microglia / Q000737:chemistry / Q000166:cytology* / Q000378:metabolism*; D018832:Molecular Chaperones / Q000032:analysis / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D013126:Spinal Nerve Roots / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D015854:Up-Regulation / Q000502:physiology*
D051152:Clusterin; D053331:Ectodysplasins; D006023:Glycoproteins; D008565:Membrane Proteins; D018832:Molecular Chaperones