Elevated activity and expression of Src-family kinases in human breast carcinoma tissue versus matched non-tumor tissue.
Src-family kinase expression was measured in 52 human mammary tumor (T) specimens compared with non-tumor (NT) tissue from the same patient by enzymatic assays employing a Src-kinase family-specific peptide substrate and by immunoblotting with an antibody recognizing the Src-family kinases Src, Fyn, and Yes. In the T specimens, the mean enzymatic activity was moderately elevated (T: 160 fmol ATP min-1 mg-1; NT: 115 fmol ATP min-1 mg-1) with 25 tumor samples having higher activity than the corresponding NT tissue, 17 having lower activity, and no activity detectable in ten T/NT pairs. Immunoblotting revealed clearly elevated expression in 25 tumor tissues and no differences or expression below the detection limit in the remaining T/NT pairs. The data are in agreement with a possible role of Src-family kinases for the biology of mammary carcinoma.
Reissig
D
D
Research Unit Molecular Cell Biology, Friedrich Schiller University, Drackendorfer Strasse 1, 07747 Jena, Germany.
Clement
J
J
Sänger
J
J
Berndt
A
A
Kosmehl
H
H
Böhmer
F D
FD
D000368:Aged; D000595:Amino Acid Sequence; D001940:Breast / Q000201:enzymology*; D001943:Breast Neoplasms / Q000201:enzymology*; D005260:Female; D006801:Humans; D015151:Immunoblotting; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D019061:src-Family Kinases / Q000378:metabolism*
D019061:src-Family Kinases