title: Developmental changes in progesterone biosynthesis and metabolism in the quail brain.
abstract: We have recently demonstrated that the quail brain possesses the cholesterol side-chain cleavage enzyme (cytochrome P450scc) and 3beta-hydroxysteroid dehydrogenase/Delta5-Delta4-isomerase (3beta-HSD) and produces pregnenolone, pregnenolone sulfate and progesterone from cholesterol. To elucidate the developmental changes in progesterone biosynthesis and its metabolism in the quail brain, we examined the expression and activity of 3beta-HSD and progesterone metabolite(s) during embryonic and post-hatched ages. Both the progesterone concentration and 3beta-HSD mRNA expression in the brain were almost constant during embryonic and post-hatched ages. The conversion of pregnenolone to progesterone (net 3beta-HSD enzymatic activity) was also constant during development and at maturity. However, without radioinert progesterone, the production of progesterone was drastically reduced in the embryonic brain, indicating active progesterone metabolism at the embryonic stage. Biochemical analysis together with HPLC and TLC revealed that only the embryonic brain actively produced 5beta-dihydroprogesterone from progesterone. Thus, progesterone production may be constant during embryonic and post-hatched development and in adulthood, whereas 5beta-dihydroprogesterone may be produced actively only in embryonic life due to 5beta-reductase.
authors: Laboratory of Brain Science, Faculty of Integrated Arts and Sciences, Hiroshima University, 739-8521, Higashi-Hiroshima, Japan.
authors: K
authors: K
authors: Ukena
authors: Y
authors: Y
authors: Honda
authors: R W
authors: RW
authors: Lea
authors: K
authors: K
authors: Tsutsui
mesh_terms: D000375:Aging / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn / Q000254:growth & development / Q000378:metabolism; D001921:Brain / Q000196:embryology* / Q000254:growth & development / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D003370:Coturnix / Q000254:growth & development* / Q000378:metabolism*; D004625:Embryo, Nonmammalian / Q000502:physiology; D009097:Multienzyme Complexes / Q000235:genetics; D011374:Progesterone / Q000378:metabolism*; D011375:Progesterone Reductase / Q000235:genetics; D012313:RNA / Q000378:metabolism; D013251:Steroid Isomerases / Q000235:genetics
chemical_list: C021425:3 beta-hydroxysteroid oxidoreductase-delta(5) 3-ketosteroid isomerase; D009097:Multienzyme Complexes; D011374:Progesterone; D012313:RNA; D011375:Progesterone Reductase; D013251:Steroid Isomerases