PCR-based microsatellite analysis for differentiation and genetic monitoring of nine inbred SENCAR mouse strains.
Sixteen DNA microsatellites or simple sequence length polymorphisms (SSLPs), generated by polymerase chain reaction (PCR) were selected for use in the genetic quality control of the nine inbred SENCAR strains currently available. The SENCAR strains constitute a powerful tool for mechanistic studies of multi-stage skin carcinogenesis, as well as for studies to understand the underlying genetic basis of resistance to tumour promotion and progression. SSLP analysis is a fast and economical way for detecting genetic contamination (unexpected outcrosses) among these closely-related albino strains, where standard immunological and biochemical markers have been shown to be insufficient.
Benavides
F
F
The University of Texas M.D. Anderson Cancer Center, Research Division, Smithville 78957, USA. fbenavides@sprd1.mdacc.tmc.edu
Glasscock
E
E
Coghlan
L G
LG
Stern
M C
MC
Weiss
D A
DA
Conti
C J
CJ
D000818:Animals; D004247:DNA / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D005819:Genetic Markers / Q000235:genetics; D051379:Mice; D018632:Mice, Inbred SENCAR / Q000145:classification / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011110:Polymorphism, Genetic / Q000235:genetics*; D011786:Quality Control
D005819:Genetic Markers; D004247:DNA