title: Kinetics of the intracellular differentiation of Leishmania amazonensis and internalization of host MHC molecules by the intermediate parasite stages.
abstract: The establishment of Leishmania in mammals depends on the transformation of metacyclic promastigotes into amastigotes within macrophages. The kinetics of this process was examined using mouse macrophages infected with metacyclic promastigotes of L. amazonensis. The appearance of amastigote characteristics, including large lysosome-like organelles called megasomes, stage-specific antigens, high cysteine protease activity and sensitivity to L-leucine methyl ester, was followed over a 5-day period. Megasomes were observed at 48 h but probable precursors of these organelles were detected at 12h p.i. The promastigote-specific molecules examined were down-regulated within 5 to 12h after phagocytosis whereas the amastigote-specific antigens studied were detectable from 2 to 12-24 h. An increase in the cysteine protease activity and in sensitivity to L-leucine methyl ester of the parasites was detected from 24 h. The data indicate that at 48 h p.i., parasites exhibit several amastigote features but that complete differentiation requires at least 5 days. The appearance of megasomes or of megasome precursors and the rise in cysteine protease activity correlate quite well with the capacity of parasites to internalize and very likely degrade host MHC molecules. The fact that internalization by the parasites of host cell molecules occurs very early during the differentiation process argues for a role of this mechanism in parasite survival.
authors: Unité d' Immunophysiologie et Parasitisme Intracellulaire, Institut Pasteur, Paris, France.
authors: N
authors: N
authors: Courret
authors: C
authors: C
authors: Frehel
authors: E
authors: E
authors: Prina
authors: T
authors: T
authors: Lang
authors: J C
authors: JC
authors: Antoine
mesh_terms: D000818:Animals; D015153:Blotting, Western / Q000662:veterinary; D002401:Cathepsin B / Q000032:analysis; D056668:Cathepsin L; D002403:Cathepsins / Q000032:analysis; D002478:Cells, Cultured; D003546:Cysteine Endopeptidases; D015536:Down-Regulation; D010450:Endopeptidases; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect / Q000662:veterinary; D000949:Histocompatibility Antigens Class II / Q000378:metabolism*; D006790:Host-Parasite Interactions / Q000276:immunology*; D007700:Kinetics; D007891:Leishmania / Q000276:immunology* / Q000472:pathogenicity; D007896:Leishmaniasis / Q000276:immunology / Q000469:parasitology / Q000662:veterinary*; D007930:Leucine / Q000031:analogs & derivatives / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D016253:Microscopy, Immunoelectron / Q000662:veterinary; D010587:Phagocytosis; D013997:Time Factors
chemical_list: D000949:Histocompatibility Antigens Class II; C030365:leucine methyl ester; D002403:Cathepsins; D010450:Endopeptidases; D003546:Cysteine Endopeptidases; D002401:Cathepsin B; D056668:Cathepsin L; C534278:Ctsl protein, mouse; D007930:Leucine