830-nm irradiation increases the wound tensile strength in a diabetic murine model.
OBJECTIVE
The purpose of this study was to investigate the effects of low-power laser irradiation on wound healing in genetic diabetes.


METHODS
Female C57BL/Ksj/db/db mice received 2 dorsal 1 cm full-thickness incisions and laser irradiation (830 nm, 79 mW/cm(2), 5.0 J/cm(2)/wound). Daily low-level laser therapy (LLLT) occurred over 0-4 days, 3-7 days, or nonirradiated. On sacrifice at 11 or 23 days, wounds were excised, and tensile strengths were measured and standardized.


RESULTS
Nontreated diabetic wound tensile strength was 0.77 +/- 0.22 g/mm(2) and 1.51 +/- 0.13 g/mm(2) at 11 and 23 days. After LLLT, over 0-4 days tensile strength was 1.15 +/- 0.14 g/mm(2) and 2.45 +/- 0.29 g/mm(2) (P = 0.0019). Higher tensile strength at 23 days occurred in the 3- to 7-day group (2.72 +/- 0.56 g/mm(2) LLLT vs. 1.51 +/- 0.13 g/mm(2) nontreated; P < or = 0.01).


CONCLUSIONS
Low-power laser irradiation at 830 nm significantly enhances cutaneous wound tensile strength in a murine diabetic model. Further investigation of the mechanism of LLLT in primary wound healing is warranted.
Stadler
I
I
The Laser Center, Rochester General Hospital, Rochester, New York 14621, USA. Istvan.Stadler@viahealth.org
Lanzafame
R J
RJ
Evans
R
R
Narayan
V
V
Dailey
B
B
Buehner
N
N
Naim
J O
JO
D000818:Animals; D004195:Disease Models, Animal; D004307:Dose-Response Relationship, Radiation; D005260:Female; D053685:Laser Therapy; D051379:Mice; D016688:Mice, Inbred NOD; D011336:Probability; D012016:Reference Values; D018570:Risk Assessment; D013718:Tensile Strength / Q000528:radiation effects*; D014945:Wound Healing / Q000502:physiology / Q000528:radiation effects*; D014947:Wounds and Injuries / Q000532:radiotherapy*