title: Characteristics of a new epidemic MRSA in Germany ancestral to United Kingdom EMRSA 15.
abstract: In 1996 a new epidemic MRSA emerged in three hospitals North of Berlin. This strain, Barnim epidemic MRSA, was isolated in 15 hospitals in Northern Germany in 1997 and 29 hospitals throughout Germany in 1998. Isolates of this clone are non-typeable by phages, its resistance phenotype is PEN, OXA, ERY, CLI, CIP (genotype: mecA, ermC, mutations in grlA and gyrA). The Sma I macrorestriction pattern corresponds to particular phage group II strains which is confirmed by the 16S-23S rRNA gene spacer pattern. Isolates of this clone differ by less than three Sma I macrorestriction fragments from isolates of the EMRSA15 clone from the United Kingdom, the most common epidemic MRSA isolates in the United Kingdom in recent years. Both epidemic strains produce enterotoxin C and possess the sec determinant for this toxin, the configuration of the mec regulon is mecI-, mecRB+, mecRC+. Both share the same Alu I pattern of PCR amplimers of the 3' end region of the coagulase gene. EMRSA 15 and Barnim EMRSA share a common multilocus sequence type indicating a recent, shared evolutionary origin.
authors: Robert Koch Institute, Wernigerode Branch, Germany. wittew@rki.de
authors: W
authors: W
authors: Witte
authors: M
authors: M
authors: Enright
authors: F J
authors: FJ
authors: Schmitz
authors: C
authors: C
authors: Cuny
authors: C
authors: C
authors: Braulke
authors: D
authors: D
authors: Heuck
mesh_terms: D015373:Bacterial Typing Techniques; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D004196:Disease Outbreaks; D019143:Evolution, Molecular; D005858:Germany / Q000453:epidemiology; D006761:Hospitals; D006801:Humans; D016106:Methicillin Resistance; D016133:Polymerase Chain Reaction; D013203:Staphylococcal Infections / Q000453:epidemiology* / Q000382:microbiology; D013211:Staphylococcus aureus / Q000145:classification / Q000187:drug effects / Q000235:genetics; D006113:United Kingdom / Q000453:epidemiology
chemical_list: C025651:CCCGGG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific