[Interspecies lysogenization in staphylococci: transfer of bacteriophage converting enterotoxin A from a clinical strain of Staphylococcus aureus to Staphylococcus intermedius].
The ability of lysogenization was examined of 50 S. intermedius strains and of 77 strains belonging to 14 different species of coagulase-negative staphylococci using 8 enterotoxin A converting bacteriophages isolated from S. aureus. All the examined bacteriophages showed lytic activity against at least 1 of 11 susceptible strains of S. intermedius to them. Lytic activity towards coagulase-negative staphylococci was observed for 6 of 8 examined bacteriophages. Two bacteriophages were active against 1 of 9 examined S. capitis strains, one against 1 of 11 examined S. haemolyticus strains, four against 1 of 6 examined S. lugdunensis strains, three against 1 of 6 examined S. warneri strains and one against 1 of 5 examined S. xylosus strains. Lysogenization with bacteriophage f421-1 able to convert positively enterotoxin A and staphylokinase and negatively beta-haemolysin of one S. intermedius strain was successful. S. intermedius lysogenized with phi 421-1 was able to produce both enterotoxin A and staphylokinase and lost ability to produce beta-haemolysin. Our results showed a broad lytic spectrum and interspecies host range of some S. aureus bacteriophages and the ability of interspecies transfer of bacteriophages between S. aureus and S. intermedius.
Zabicka
D
D
Katedra i Zakład Mikrobiologii Lekarskiej, Centrum Biostruktury Akademii Medycznej w Warszawie.
Młynarczyk
G
G
Łuczak
M
M
D004768:Enterotoxins / Q000096:biosynthesis; D006460:Hemolysin Proteins / Q000096:biosynthesis; D008242:Lysogeny; D008666:Metalloendopeptidases / Q000096:biosynthesis; D013045:Species Specificity; D013210:Staphylococcus / Q000378:metabolism* / Q000821:virology*; D013204:Staphylococcus Phages / Q000502:physiology*; D013211:Staphylococcus aureus / Q000378:metabolism / Q000821:virology
D004768:Enterotoxins; D006460:Hemolysin Proteins; C026066:enterotoxin A, Staphylococcal; D008666:Metalloendopeptidases; C022653:auR protein, Staphylococcus aureus