title: Specificity of carbohydrate structures of gangliosides in the activity to regenerate the rat axotomized hypoglossal nerve.
abstract: We previously reported that a ganglioside mixture from bovine brain could prevent neuronal death and promote regeneration in rats with hypoglossal nerve resection. In the present study, we have compared the neurotrophic effects of various glycosphingolipids including lactosyl-ceramide. The findings revealed that GT1b had the activity of neuronal death prevention equivalent to a ganglioside mixture or autograft, while other glycolipids exhibited about 60% activity. However, the capability to promote the regeneration varied among glycolipids, that is, GT1b (86%), GD1b (55%), GD1a (35%), GQ1b (34%), GM1 (20%), lactosyl-ceramide (17%) in the number of horseradish peroxidase-positive neurons as an indicator of regeneration. The experiments with oligosaccharides of GT1b or GD1b and ceramide showed that the carbohydrate moiety mainly exerts neurotrophic effects. These findings suggested that fine structures of carbohydrate moiety in gangliosides are critical in the regenerative activity in this hypoglossal nerve regeneration system.
authors: First Department of Oral and Maxillofacial Surgery, Nagasaki University School of Dentistry, 1-7-1, Sakamoto, Nagasaki, 852-8102, Japan.
authors: M
authors: M
authors: Itoh
authors: S
authors: S
authors: Fukumoto
authors: T
authors: T
authors: Iwamoto
authors: A
authors: A
authors: Mizuno
authors: A
authors: A
authors: Rokutanda
authors: H K
authors: HK
authors: Ishida
authors: M
authors: M
authors: Kiso
authors: K
authors: K
authors: Furukawa
mesh_terms: D000818:Animals; D019771:Axotomy; D002236:Carbohydrate Conformation; D005732:Gangliosides / Q000737:chemistry / Q000502:physiology*; D007002:Hypoglossal Nerve / Q000502:physiology* / Q000601:surgery; D007150:Immunohistochemistry; D009416:Nerve Regeneration; D051381:Rats; D017208:Rats, Wistar
chemical_list: D005732:Gangliosides