Enhancement of the virulence of Aeromonas caviae diarrhoeal strains by serial passages in mice.
Thirteen clinical Aeromonas caviae isolates from the faeces of 13 children with mild to severe diarrhoea were tested for enhancement of mouse lethality, adhesion ability, siderophore and cholera toxin cross-reactive (CTC) factor production, by four consecutive passages through mice by intraperitoneal injection of A. caviae suspensions. The passaged A. caviae strains were re-isolated from monomicrobic cardiac blood samples and inocula were prepared for the next passage. All A. caviae isolates possessed the ability to adhere to the mucosal epithelial surface of the rabbit small intestine. Serial passage in mice showed that the virulence of some isolates for mice was increased in terms of percentage mortality and a lowering of the LD50. For some of the isolates, but not all, serial passage appeared to increase siderophore production and adhesion to rabbit small intestinal cells. For the A. caviae isolates tested, increased values of the CTC factor were observed after passage. A clear correlation was observed between the lowering of LD50 and the enhancement of CTC factor production after passage in mice. These results indicate that the A. caviae isolates possessed virulence factors.
Krzymińska
Sylwia
S
Department of Microbiology, Experimental Biology Institute, University of Poznań, Poznań 61-701, Poland.
Mokracka
Joanna
J
Department of Microbiology, Experimental Biology Institute, University of Poznań, Poznań 61-701, Poland.
Łaganowska
Marzena
M
Department of Microbiology, Experimental Biology Institute, University of Poznań, Poznań 61-701, Poland.
Włodarczak
Krystyna
K
Department of Microbiology, Experimental Biology Institute, University of Poznań, Poznań 61-701, Poland.
Guszczyńska
Elż Bieta
EB
Department of Microbiology, Experimental Biology Institute, University of Poznań, Poznań 61-701, Poland.
Liszkowska
Joanna
J
Department of Microbiology, Experimental Biology Institute, University of Poznań, Poznań 61-701, Poland.
Popkowska
Ewa
E
Department of Microbiology, Experimental Biology Institute, University of Poznań, Poznań 61-701, Poland.
Lima
Izilda
I
Department of Microbiology, Experimental Biology Institute, University of Poznań, Poznań 61-701, Poland.
Lemańska
Iza
I
Department of Microbiology, Experimental Biology Institute, University of Poznań, Poznań 61-701, Poland.
Wendt
Małgorzata
M
Department of Microbiology, Experimental Biology Institute, University of Poznań, Poznań 61-701, Poland.
D000333:Aeromonas / Q000472:pathogenicity* / Q000502:physiology*; D000818:Animals; D001422:Bacterial Adhesion; D001427:Bacterial Toxins / Q000276:immunology; D002648:Child; D002772:Cholera Toxin / Q000276:immunology; D003429:Cross Reactions; D003470:Culture Media; D003967:Diarrhea / Q000382:microbiology*; D004195:Disease Models, Animal; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology* / Q000401:mortality; D006801:Humans; D007928:Lethal Dose 50; D051379:Mice; D011817:Rabbits; D012692:Serial Passage; D017262:Siderophores / Q000378:metabolism; D013499:Surface Properties; D014774:Virulence
D001427:Bacterial Toxins; D003470:Culture Media; D017262:Siderophores; D002772:Cholera Toxin