title: A capillary electrophoresis chip for the analysis of print and film photographic developing agents in commercial processing solutions using indirect fluorescence detection.
abstract: The separation and detection of both print and film developing agents (CD-3 and CD-4) in photographic processing solutions using chip-based capillary electrophoresis is presented. For simultaneous detection of both analytes under identical experimental conditions a buffer pH of 11.9 is used to partially ionise the analytes. Detection is made possible by indirect fluorescence, where the ions of the analytes displace the anionic fluorescing buffer ion to create negative peaks. Under optimal conditions, both analytes can be analyzed within 30 s. The limits of detection for CD-3 and CD-4 are 0.17 mM and 0.39 mM, respectively. The applicability of the method for the analysis of seasoned photographic processing developer solutions is also examined.
authors: AstraZeneca/SmithKline Beecham Centre for Analytical Sciences, Department of Chemistry, Imperial College of Science, Technology and Medicine, South Kensington, London, UK.
authors: S
authors: S
authors: Sirichai
authors: A J
authors: AJ
authors: de Mello
mesh_terms: D002021:Buffers; D019075:Electrophoresis, Capillary / Q000295:instrumentation*; D004867:Equipment Design; D005470:Fluorometry / Q000379:methods*; D008832:Microchemistry / Q000295:instrumentation*; D008904:Miniaturization; D010781:Photography / Q000295:instrumentation*; D012680:Sensitivity and Specificity; D012996:Solutions; D013449:Sulfonamides / Q000032:analysis*; D014050:Toluene / Q000031:analogs & derivatives* / Q000032:analysis*
chemical_list: D002021:Buffers; D012996:Solutions; D013449:Sulfonamides; C041977:4-amino-N-ethyl-N-(beta-hydroxy-ethyl)-m-toluidine; D014050:Toluene; C041213:CD 3 color developer