Expression of CD61 (beta3 integrin subunit) on canine cells.
A monoclonal antibody (JM2E5) specific for the integrin beta3 chain, or CD61 or GPIIIa subunit, has been employed to determine the expression of the canine homologue CD41/CD61 or CD51/CD61 complex on different canine cells in peripheral blood lymphocytes, monocytes, granulocytes, platelets, erythrocytes, lymph-node cells, spleen cells and breast tumour cells). The canine homologue CD41/CD61 or CD51/61 was present on peripheral blood lymphocytes, monocytes, granulocytes, breast tumour cells and spleen cells as well as on platelets and it was absent from erythrocytes and lymph-node cells. An antigen with components of molecular masses of 25/100/120 kDa (under reducing conditions) was immunoprecipitated from canine peripheral lymphocytes and platelets, but not from granulocytes or monocytes. Expression on canine lymphocytes of the canine homologue of the human beta3 integrin chain was unexpected, based on the expression pattern of this molecule in human tissue.
Arce
C
C
Departamento de Genética, Facultad de Veterinaria, Universidad de Córdoba, Spain.
Moreno
A
A
Pérez de la Lastra
J M
JM
Garrido
J J
JJ
Barbancho
M
M
De Andrés
D F
DF
Morera
L
L
Llanes
D
D
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D015703:Antigens, CD / Q000032:analysis* / Q000097:blood / Q000737:chemistry / Q000276:immunology; D001773:Blood Cells / Q000737:chemistry*; D004283:Dog Diseases / Q000097:blood / Q000473:pathology; D004285:Dogs / Q000097:blood*; D005434:Flow Cytometry; D006801:Humans; D039661:Integrin beta3; D008198:Lymph Nodes / Q000166:cytology; D008214:Lymphocytes / Q000737:chemistry*; D015674:Mammary Neoplasms, Animal / Q000097:blood / Q000473:pathology; D008970:Molecular Weight; D014411:Neoplastic Stem Cells / Q000737:chemistry*; D009928:Organ Specificity; D019039:Platelet Glycoprotein GPIIb-IIIa Complex / Q000032:analysis; D010980:Platelet Membrane Glycoproteins / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D013045:Species Specificity; D013154:Spleen / Q000166:cytology
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D039661:Integrin beta3; D019039:Platelet Glycoprotein GPIIb-IIIa Complex; D010980:Platelet Membrane Glycoproteins