Differences between Desulfovibrio desulfuricans intestinal strains with respect to their ability of sulphasalazine biotransformation.
In presented study, differences among six intestinal Desulfovibrio desulfuricans strains were investigated regarding their ability of sulphasalazine (SAS) biotransformation. Bacterial strains were incubated (24 or 48 h) in liquid medium supplemented with SAS (12.2-150 microM). It has been demonstrated that investigated D. desulfuricans strains converted SAS with various rates, depending on the strain used and on time of drug contact with bacterial cells. Significantly different (50% coefficient of variability) were the maximum rates of SAS biotransformation. It was demonstrated that these rates were strongly dependent on the drug concentration. Increasing SAS concentrations up to about 120 microM caused increase in the drug transformation rate. At higher SAS concentrations this rate remained at the same level or was inconsiderable lower. The strains exposed to SAS for 24 hs transformed this drug with the rate about twice as compared with that observed after 48 h exposition.
Dzierzewicz
Z
Z
Department of Molecular Biology, Biochemistry and Biopharmacy Medical University of Silesia, 1 Narcyzów Str., 41-200 Sosnowiec.
Cwalina
B
B
Kuśmierz
D
D
Kwapisz
I
I
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000493:pharmacokinetics*; D001711:Biotransformation; D003901:Desulfovibrio / Q000378:metabolism*; D006801:Humans; D007422:Intestines / Q000382:microbiology*; D012460:Sulfasalazine / Q000493:pharmacokinetics*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D012460:Sulfasalazine