Age-related regional difference of interleukin-1 expression in rat brain after lipopolysaccharide treatment.
Aging is associated with altered immune responses including dysregulation of cytokine production. Of cytokines, interleukin-1 (IL-1) family has been primarily involved with central nervous system. To evaluate the age-related different response of IL-1 family following peripheral administration of lipopolysaccharide (LPS), immunohistochemical study of IL-1beta and IL-1 receptor expression was performed on Sprague-Dawley rat brain. Experimental animals were divided into four groups; saline-treated young (3-5 months) and old (over 24 months), and LPS-treated young and old groups. After intraperitoneal (i.p.) injection of LPS, three to five rats within each group were killed at 1, 2, 4, 8 and 16 hr. After fixation in 4% neutral buffered formalin, the brain slices were paraffin-embedded. Immunohistochemical staining using labelled streptavidin biotin was performed. The results showed that IL-1beta immunoreactivity was seen in the endothelial cell of pons in both LPS-treated young and old rats, with slightly longer persistency in old group. IL-1RI immunoreactivity appeared initially in the neurons of cerebral cortex in LPS-treated old group, compared with predominantly the cerebellum in LPS-treated young group. In conclusion, our study shows that there is age-related, different neuronal localization of IL-1RI expression at different points of time after LPS treatment.
Huh
G Y
GY
Department of Pathology, Dong-A University College of Medicine, Pusan, Korea. gyhuh@daunet.donga.ac.kr
Roh
M S
MS
Bae
H R
HR
D000367:Age Factors; D000818:Animals; D001923:Brain Chemistry / Q000187:drug effects*; D005786:Gene Expression Regulation / Q000187:drug effects; D007150:Immunohistochemistry; D007375:Interleukin-1 / Q000032:analysis* / Q000235:genetics; D008070:Lipopolysaccharides / Q000633:toxicity*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D017472:Receptors, Interleukin-1 / Q000032:analysis
D007375:Interleukin-1; D008070:Lipopolysaccharides; D017472:Receptors, Interleukin-1