Use of bioimpedance spectroscopy to estimate body water distribution in rats fed high dietary sulfur amino acids.
The effect of dietary sulfur amino acids on bioelectric properties was studied in rats by using bioimpedance spectroscopy. Weanling rats were assigned to one of 12 groups in a factorially arranged experiment with dietary variables of supplemental sulfur amino acid (none, 10 g DL-methionine/kg or 10 g DL-homocystine/kg), pyridoxine hydrochloride (0 or 7.5 mg/kg) and nickel (0 or 1 mg/kg). After 9 wk of feeding, 20-h urine specimens were collected from food-deprived rats for measurements of creatinine, and then bioimpedance was measured with multifrequency (Hydra ECF/ICF 4200) and single-frequency (RJL Systems model 101) analyzers. Urinary creatinine excretion was measured by intracellular water (ICW), total body solid and urinary volume (R2 = 0.675). Extracellular water (ECW) did not add significantly to the model. Rats fed methionine had significantly lower total body water, ICW and ECW than rats fed no supplemental sulfur amino acid. Rats fed homocystine had significantly lower ECW and a significantly higher ratio of ICW to ECW. Rats fed methionine or homocystine had significantly lower capacitance corrected for body length and ICW than those fed no supplemental sulfur amino acids. These results suggest that dietary homocystine changes the distribution of body water and that sulfur amino acids can affect membrane porosity and/or membrane thickness.
Yokoi
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K
U.S. Department of Agriculture, Agricultural Research Service, Grand Forks Human Nutrition Research Center, Grand Forks, North Dakota 58202-9034, USA. kyokoi@gfhnrc.ars.usda.gov
Lukaski
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HC
Uthus
E O
EO
Nielsen
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FH
D000603:Amino Acids, Sulfur / Q000008:administration & dosage* / Q000494:pharmacology; D000818:Animals; D001823:Body Composition / Q000187:drug effects; D001834:Body Water / Q000378:metabolism*; D003404:Creatinine / Q000652:urine; D004032:Diet; D017097:Electric Impedance; D005110:Extracellular Space / Q000378:metabolism; D006711:Homocystine / Q000008:administration & dosage / Q000494:pharmacology; D007424:Intracellular Fluid / Q000378:metabolism; D008297:Male; D008715:Methionine / Q000008:administration & dosage / Q000494:pharmacology; D009532:Nickel / Q000494:pharmacology; D011736:Pyridoxine / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D014018:Tissue Distribution
D000603:Amino Acids, Sulfur; D006711:Homocystine; D009532:Nickel; D008715:Methionine; D003404:Creatinine; D011736:Pyridoxine