Chemistry of sulforhodamine--amine conjugates.
Commercially-available sulforhodamine sulfonyl chlorides contain two isomeric monosulfonyl chlorides. Conjugates of these isomers with amines have different properties because the sulfonamide formed from one isomer can undergo ring-closure to a colorless sultam. This chemistry has been examined for a model conjugate with methylamine and for a bioconjugate with 2'(3')-O-[N-(2-aminoethyl)carbamoyl]ATP. The interaction of each isomer of the latter conjugates with myosin subfragment 1 has been characterized. Significant differences between the two isomers are observed in these interactions.
Corrie
J E
JE
National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK. jcorrie@nimr.mrc.ac.uk
Davis
C T
CT
Eccleston
J F
JF
D000244:Adenosine Diphosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry*; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D008744:Methylamines / Q000737:chemistry*; D015394:Molecular Structure; D012235:Rhodamines / Q000737:chemistry*; D013057:Spectrum Analysis; D014966:Xanthenes / Q000737:chemistry*
D005456:Fluorescent Dyes; D008744:Methylamines; D012235:Rhodamines; D014966:Xanthenes; D000244:Adenosine Diphosphate; C034657:Texas red; D000255:Adenosine Triphosphate