The effect of temperature oscillations on DNA sequencing by capillary electrophoresis.
Although capillary electrophoresis is a powerful sequencing technology, the low heat capacity of a capillary can make difficult the precise control of its temperature, particularly when the capillary is heated to reduce compressions in the separation of DNA sequencing fragments. In this paper, we demonstrate that minute oscillations in the capillary's temperature result in significant degradation in the number of theoretical plates, the resolution between adjacent peaks, and the number of bases of DNA sequence determined from the electrophoresis data. Temperature must be held stable to within 0.1 degrees C to obtain long read lengths. A Monte Carlo simulation demonstrates that this degradation is consistent with laminar flow induced by the periodic thermal expansion and contraction of the separation medium.
Voss
K O
KO
Department of Chemistry, University of Alberta, Edmonton, Canada.
Roos
H P
HP
Dovichi
N J
NJ
D019075:Electrophoresis, Capillary; D007700:Kinetics; D009010:Monte Carlo Method; D017422:Sequence Analysis, DNA / Q000379:methods*; D013696:Temperature