title: Aetiology of attenuated luteal development in prednisolone-induced eosinopenic ewes.
abstract: Eosinophilic leukocytes infiltrate the wall of postovulatory ovine follicles. The objective of this investigation was to assess a putative role of resident eosinophils in the folliculo luteal transition. Eosinophils accumulated where new blood vessels were evident along connective tissue trabeculae that pervaded the parenchyma of formative corpora lutea. Mid-phase function of corpora lutea (progesterone output) was suppressed in ewes in which eosinophils were ablated by systemic administration of prednisolone following ovulation. Glandular dysfunction was related to a diminished angiogenic response (quantitative image analysis of vascular space in histological specimens and scanning electron microscopy of corrosion casts) during the luteinization process. Vascular endothelial growth factor (VEGF) was localized by immunofluorescence microscopy to luteal eosinophils. It is suggested that VEGF of eosinophilic origin contributes to the neovascularization mechanism of corpora lutea in sheep.
authors: Department of Animal Science, University of Wyoming, Laramie 82071, USA. wmurdoch@uwyo.edu
authors: W J
authors: WJ
authors: Murdoch
authors: E A
authors: EA
authors: Van Kirk
mesh_terms: D000818:Animals; D003338:Corpus Luteum / Q000098:blood supply / Q000187:drug effects* / Q000254:growth & development* / Q000502:physiology; D016319:Corrosion Casting; D016228:Endothelial Growth Factors / Q000502:physiology; D004804:Eosinophils / Q000187:drug effects* / Q000502:physiology; D005260:Female; D007970:Leukopenia / Q000139:chemically induced* / Q000503:physiopathology*; D008183:Luteal Phase / Q000187:drug effects* / Q000502:physiology*; D008222:Lymphokines / Q000502:physiology; D008855:Microscopy, Electron, Scanning; D018919:Neovascularization, Physiologic / Q000187:drug effects; D011239:Prednisolone / Q000494:pharmacology*; D012756:Sheep; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
chemical_list: D016228:Endothelial Growth Factors; D008222:Lymphokines; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; D011239:Prednisolone