Limited signal transduction repertoire of human Y(5) neuropeptide Y receptors expressed in HEC-1B cells.
In HEC-1B cells transfected with human Y(5) neuropeptide Y (NPY) receptors (but not in non-transfected cells) NPY inhibited forskolin-stimulated cAMP accumulation in a pertussis toxin-sensitive manner (-log EC(50) 8.88 +/- 0.25). Elevations of intracellular Ca(2+) were largely restricted to very high NPY concentrations and similar in transfected and nontransfected cells. NPY did not increase inositol phosphate accumulation and did not activate a variety of isoforms of protein kinase C or mitogen-activated protein kinases. We conclude that at least upon expression in HEC-1B cells the signal transduction of Y(5) NPY receptors is limited to inhibition of cAMP accumulation.
Bischoff
A
A
Department of Medicine, University of Essen, 45122, Essen, Germany.
Püttmann
K
K
Kötting
A
A
Moser
C
C
Buschauer
A
A
Michel
M C
MC
D000704:Analysis of Variance; D000818:Animals; D016466:CHO Cells; D019556:COS Cells; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D005576:Colforsin / Q000378:metabolism; D006224:Cricetinae; D000242:Cyclic AMP / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation; D006801:Humans; D007295:Inositol Phosphates / Q000378:metabolism; D020935:MAP Kinase Signaling System; D037342:Pertussis Toxin; D010710:Phosphates / Q000378:metabolism; D020033:Protein Isoforms; D011493:Protein Kinase C / Q000737:chemistry / Q000378:metabolism; D017476:Receptors, Neuropeptide Y / Q000737:chemistry* / Q000378:metabolism*; D015398:Signal Transduction; D013997:Time Factors; D014162:Transfection; D010566:Virulence Factors, Bordetella / Q000378:metabolism
D007295:Inositol Phosphates; D010710:Phosphates; D020033:Protein Isoforms; D017476:Receptors, Neuropeptide Y; D010566:Virulence Factors, Bordetella; C100568:neuropeptide Y5 receptor; D005576:Colforsin; D000242:Cyclic AMP; D037342:Pertussis Toxin; D011493:Protein Kinase C; D002118:Calcium