title: Anaerobiosis induces complex changes in sterol esterification pattern in the yeast Saccharomyces cerevisiae.
abstract: Yeast Saccharomyces cerevisiae is auxotrophic for ergosterol in the absence of oxygen. We showed that complex changes in esterification of exogenously supplied sterols were also induced by anaerobiosis. Utilization of oleic acid for sterol esterification was significantly impaired in anaerobic cells. Furthermore, anaerobic cells fed different sterols exhibited striking variation in esterification efficiency (high levels of sterol esters for cholesterol and sitosterol, low levels for ergosterol, lanosterol or stigmasterol). Relative activities of two yeast acylCoA:sterol acyltransferases (Are1p and Are2p) changed in response to anaerobiosis: while Are2p was dominant under aerobic conditions, Are1p provided the major activity in the absence of oxygen. Our results indicate that sterol esters may fulfil different roles in aerobic and anaerobic cells.
authors: Institute of Animal Biochemistry and Genetics, Slovak Academy of Sciences, 900 28, Ivanka pri Dunaji, Slovak Republic.
authors: M
authors: M
authors: Valachovic
authors: L
authors: L
authors: Hronská
authors: I
authors: I
authors: Hapala
mesh_terms: D000693:Anaerobiosis; D003470:Culture Media; D004875:Ergosterol / Q000378:metabolism; D004951:Esterification; D019301:Oleic Acid / Q000378:metabolism; D010100:Oxygen / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000254:growth & development; D002785:Sterol O-Acyltransferase / Q000378:metabolism*; D013261:Sterols / Q000378:metabolism*
chemical_list: D003470:Culture Media; D013261:Sterols; D019301:Oleic Acid; D002785:Sterol O-Acyltransferase; D010100:Oxygen; D004875:Ergosterol