title: Design of novel zinc finger proteins: towards artificial control of specific gene expression.
abstract: In this review, we summarize design strategies for generating proteins with desired sequences such as long contiguous base pairs and diverse sequence specificities based on the nature of Cys(2)-His(2) zinc finger proteins. Recent progress towards artificial DNA binding proteins has been achieved by structure-based design processes and by selection strategies. Indeed, a multi-zinc finger protein with an 18 (or 27)-base pair address, and new zinc finger proteins for diverse DNA target sites (TATA-box and p53 binding site) have been created successfully. Such novel zinc finger proteins will probably be useful tools in molecular biology and potentially in human medicine.
authors: Institute for Chemical Research, Kyoto University, Uji, 611-0011, Kyoto, Japan.
authors: M
authors: M
authors: Imanishi
authors: Y
authors: Y
authors: Hori
authors: M
authors: M
authors: Nagaoka
authors: Y
authors: Y
authors: Sugiura
mesh_terms: D000818:Animals; D004247:DNA / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D051766:Early Growth Response Protein 1; D015870:Gene Expression / Q000502:physiology*; D016158:Genes, p53 / Q000502:physiology; D006801:Humans; D017874:Immediate-Early Proteins; D016385:TATA Box / Q000502:physiology; D014157:Transcription Factors / Q000737:chemistry / Q000378:metabolism*; D015533:Transcriptional Activation / Q000187:drug effects / Q000502:physiology*
chemical_list: D004268:DNA-Binding Proteins; C497325:EGR1 protein, human; D051766:Early Growth Response Protein 1; D017874:Immediate-Early Proteins; D014157:Transcription Factors; D004247:DNA