Contribution of CD8(+) T cells to gamma interferon production in human tuberculosis.
The proportions of peripheral blood mononuclear cells (PBMC), CD4(+) T cells, and CD8(+) T cells that produce gamma interferon (IFN-gamma) in response to Mycobacterium tuberculosis were markedly reduced in tuberculosis patients, particularly in those with severe disease. Depletion of CD4(+) but not CD8(+) cells prior to stimulation of PBMC with M. tuberculosis abolished IFN-gamma production. These results show that (i) IFN-gamma production by CD8(+) and CD4(+) cells correlates with the clinical manifestations of M. tuberculosis infection and (ii) IFN-gamma production by CD8(+) cells depends on CD4(+) cells.
Shams
H
H
Center for Pulmonary and Infectious Disease Control, University of Texas Health Center, Tyler 75708-3154, USA.
Wizel
B
B
Weis
S E
SE
Samten
B
B
Barnes
P F
PF
D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000378:metabolism*; D002478:Cells, Cultured; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis*; D008212:Lymphocyte Depletion; D014376:Tuberculosis / Q000276:immunology*
D007371:Interferon-gamma