title: Human thymus contains IFN-alpha-producing CD11c(-), myeloid CD11c(+), and mature interdigitating dendritic cells.
abstract: Three distinct dendritic cell (DC) subsets capable of stimulating allogeneic naive T cells were isolated from human thymus. The most abundant subset was represented by plasmacytoid DCs (pDCs), which secreted high amounts of IFN-alpha upon stimulation with inactivated influenza virus and thus likely correspond to the recently identified peripheral blood natural IFN-alpha/beta-producing cells (IPCs). Like those latter cells, thymic pDCs had distinctive phenotypic features (i.e., Lin(-), HLA-DR(int), IL-3R alpha(hi), CD45RA(hi), CD11c(-), CD13(-), and CD33(lo)) and developed into mature DCs upon culture in IL-3 and CD40L. Of the two other DC subsets, one displayed a phenotype of immature myeloid DCs (imDCs) (HLA-DR(int), CD11c(+), CD13(+), CD33(+)), and the other represented HLA-DR(hi) CD11c(+) mature DCs (mDCs). Since they also expressed DC-LAMP, these mDCs appear to correspond to interdigitating dendritic cells (IDCs). Thymic pDCs, but not myeloid imDCs, strongly expressed lymphoid-specific transcripts such as pre-T alpha, lambda-like, and Spi-B, thereby suggesting a possible lymphoid origin. The detection of Spi-B mRNA, not only upon in vitro maturation of pDCs, but also in freshly purified IDCs, suggests that in vivo pDCs may differentiate into IDCs.
authors: Schering-Plough, Laboratory for Immunological Research, Dardilly, France.
authors: N
authors: N
authors: Bendriss-Vermare
authors: C
authors: C
authors: Barthélémy
authors: I
authors: I
authors: Durand
authors: C
authors: C
authors: Bruand
authors: C
authors: C
authors: Dezutter-Dambuyant
authors: N
authors: N
authors: Moulian
authors: S
authors: S
authors: Berrih-Aknin
authors: C
authors: C
authors: Caux
authors: G
authors: G
authors: Trinchieri
authors: F
authors: F
authors: Brière
mesh_terms: D000293:Adolescent; D023201:CD40 Ligand / Q000494:pharmacology; D002469:Cell Separation; D002648:Child; D002675:Child, Preschool; D003713:Dendritic Cells / Q000145:classification* / Q000276:immunology / Q000378:metabolism; D006801:Humans; D007223:Infant; D016167:Integrin alphaXbeta2; D016898:Interferon-alpha / Q000494:pharmacology; D007377:Interleukin-3 / Q000494:pharmacology; D009975:Orthomyxoviridae / Q000276:immunology; D012333:RNA, Messenger; D016185:Receptors, Interleukin-3 / Q000235:genetics; D013950:Thymus Gland / Q000166:cytology*
chemical_list: D016167:Integrin alphaXbeta2; D016898:Interferon-alpha; D007377:Interleukin-3; D012333:RNA, Messenger; D016185:Receptors, Interleukin-3; D023201:CD40 Ligand