Molecular characterization in the VP7, VP4 and NSP4 genes of human rotavirus serotype 4 (G4) isolated in Japan and Kenya.
The VP7, VP4 and NSP4 genes of human rotavirus serotype 4 (G4) were analyzed to investigate intraserotypic variations. The techniques used included reverse transcription polymerase chain reaction with subtype specific primers, restriction fragment length polymorphism analysis and sequence analysis. Twelve isolates (nine from Japan and three from Kenya) and two standard strains (Hochi, Odelia) were G4A P[8] Wa group NSP4. A standard strain (ST3) was G4A P[6] Wa group NSP4 and a strain (VA70) was G4B P[8] Wa group NSP4. These results show G4 rotaviruses can be divided into three combinations at the moment.
Kudo
S
S
Department of Pediatrics, Teikyo University School of Medicine, Tokyo, Japan.
Zhou
Y
Y
Cao
X R
XR
Yamanishi
S
S
Nakata
S
S
Ushijima
H
H
D000956:Antigens, Viral; D002213:Capsid / Q000235:genetics*; D036022:Capsid Proteins; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D005814:Genes, Viral / Q000235:genetics; D014644:Genetic Variation; D006023:Glycoproteins / Q000235:genetics*; D006801:Humans; D007564:Japan / Q000453:epidemiology; D007630:Kenya / Q000453:epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012401:Rotavirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000453:epidemiology / Q000821:virology*; D012703:Serotyping; D014118:Toxins, Biological; D017361:Viral Nonstructural Proteins / Q000235:genetics*
D000956:Antigens, Viral; D036022:Capsid Proteins; D004279:DNA, Viral; D006023:Glycoproteins; C081369:NS28 protein, rotavirus; D014118:Toxins, Biological; C060339:VP4 protein, Rotavirus; C050779:VP7 protein, Rotavirus; D017361:Viral Nonstructural Proteins