Expression of vascular endothelial growth factor during the early stage of maxillofacial blast injury.
OBJECTIVE
To determine the expression and the change of vascular endothelial growth factor (VEGF) and its acceptor KDR during the early stage of maxillofacial blast injury. To evaluate the effect of VEGF on traumatic wound healing.


METHODS
The rabbit model of maxillofacial blast injury was made by KTY-04 blasting cap. The expression of VEGF and KDR in wound tissue was determined by ABC immunohistochemistry of 6 hours, 1 day, 2 days, 3 days, 5 days, and 7 days after injury. The results were compared with preinjury groups.


RESULTS
In the wound tissue of the maxillofacial blast injury, the expression of VEGF rose steadily during the first week after the injury. Compared with normal tissue, the wound tissue showed differences by the first day after injury, and showed significant differences by the third day (P < 0.01). The differences reached a peak at the seventh day after injury. The expression of KDR showed no difference within 3 days after injury when compared to normal tissue. Between 5 and 7 days after injury, the protein was more strongly expressed.


CONCLUSIONS
The stage of the VEGF expression at the maxillofacial blast injury site is similar to the angiopoietic stage during would healing, and KDR expression also occurs during that period. VEGF takes part in angiogenic cascades of traumatic wound healing and is produced as an auxiliary action to the regeneration of blood vessels.
Zhang
C J
CJ
Department of Stomatology (DS), Southwest Hospital (SH), Third Military Medical University (TMMU), Chongqing City, P. R. China.
Li
H Z
HZ
Zhou
S X
SX
Yang
J
J
Huang
Q Y
QY
D000818:Animals; D001753:Blast Injuries / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D003238:Connective Tissue / Q000378:metabolism / Q000473:pathology; D004195:Disease Models, Animal; D016228:Endothelial Growth Factors / Q000032:analysis* / Q000235:genetics; D004730:Endothelium, Vascular / Q000378:metabolism / Q000473:pathology; D004848:Epithelium / Q000378:metabolism / Q000473:pathology; D005500:Follow-Up Studies; D015870:Gene Expression; D007091:Image Processing, Computer-Assisted; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D008222:Lymphokines / Q000032:analysis* / Q000235:genetics; D008446:Maxillofacial Injuries / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D018919:Neovascularization, Physiologic / Q000235:genetics; D020033:Protein Isoforms / Q000032:analysis* / Q000235:genetics; D011817:Rabbits; D011897:Random Allocation; D020794:Receptor Protein-Tyrosine Kinases / Q000032:analysis / Q000235:genetics; D017978:Receptors, Growth Factor / Q000032:analysis / Q000235:genetics; D011975:Receptors, Mitogen / Q000032:analysis / Q000235:genetics; D040262:Receptors, Vascular Endothelial Growth Factor; D012038:Regeneration / Q000235:genetics; D017695:Soft Tissue Injuries / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D013223:Statistics as Topic; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; D014945:Wound Healing / Q000502:physiology
D016228:Endothelial Growth Factors; D008222:Lymphokines; D020033:Protein Isoforms; D017978:Receptors, Growth Factor; D011975:Receptors, Mitogen; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; D020794:Receptor Protein-Tyrosine Kinases; D040262:Receptors, Vascular Endothelial Growth Factor