Investigation of the ternary D-myo-inositol 1,2,6-tris(phosphate)-spermine-Zn2+ system in solution.
Interactions of Ins(1,2,6)P3 (IP), with spermine (Spm) and zinc cations have been studied by potentiometric and 31P NMR titrations. In the 4-11 pH range, two IPSpmZn2H3 and IPSpmZn2H mixed complexes are formed which are largely predominant with respect to the binary species. According to 31P NMR titration it is likely that one of the zinc cations preferably binds phosphates P1 and P6. The adduct formation between Ins(1,2,6)P3 and spermine seems also favourable to the formation of the mixed complexes. The occurrence of ternary complexes involving inositol-phosphates, biogenic amines, and metallic cations may be of relevance in the regulation of biological processes.
Felemez
M
M
Laboratoire de Pharmacochimie Moléculaire, UMR 7081 du CNRS, Faculté de Pharmacie, Illkirch, France.
Spiess
B
B
D066298:In Vitro Techniques; D007295:Inositol Phosphates / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D011199:Potentiometry; D015290:Second Messenger Systems; D012996:Solutions; D013096:Spermine / Q000737:chemistry*; D015032:Zinc / Q000737:chemistry*
D007295:Inositol Phosphates; D012996:Solutions; D013096:Spermine; D015032:Zinc; C059368:atrinositol