title: Laboratory diagnosis of Clostridium difficile-associated diarrhea and colitis: usefulness of Premier Cytoclone A+B enzyme immunoassay for combined detection of stool toxins and toxigenic C. difficile strains.
abstract: Detection of Clostridium difficile toxins A and B in stools by Premier Cytoclone A+B enzyme immunoassay (EIA) was compared with detection by stool culture for C. difficile followed by detection of toxigenic isolates using the same EIA. Chart reviews were performed to evaluate the likelihood of C. difficile-associated diarrhea and colitis (CADC) for all patients with at least one positive toxin assay. While the toxins were detected in 58 of 85 consecutive CADC patients by both assays, CADC in 5 patients was detected only by stool toxin assay, and in 22 patients CADC was detected only by toxigenic culture. Our results suggest that for laboratories using a rapid toxin A+B EIA, direct toxin detection in stools should be combined with toxigenic culture in cases in which there is a negative stool toxin assay.
authors.affiliation: Laboratoire de Bactériologie, Hôpital Central, 29, Avenue du Maréchal de Lattre de Tassigny, Centre Hospitalier et Universitaire, 54035 Nancy Cedex, France.
authors.forename: A
authors.initials: A
authors.lastname: Lozniewski
authors.forename: C
authors.initials: C
authors.lastname: Rabaud
authors.forename: E
authors.initials: E
authors.lastname: Dotto
authors.forename: M
authors.initials: M
authors.lastname: Weber
authors.forename: F
authors.initials: F
authors.lastname: Mory
mesh_terms: D001426:Bacterial Proteins; D001427:Bacterial Toxins / Q000032:analysis*; D016360:Clostridioides difficile / Q000302:isolation & purification*; D003470:Culture Media; D003967:Diarrhea / Q000175:diagnosis* / Q000382:microbiology; D004761:Enterocolitis, Pseudomembranous / Q000175:diagnosis* / Q000382:microbiology; D004768:Enterotoxins / Q000032:analysis*; D005243:Feces / Q000737:chemistry; D006801:Humans; D007124:Immunoenzyme Techniques
chemical_list: D001426:Bacterial Proteins; D001427:Bacterial Toxins; D003470:Culture Media; D004768:Enterotoxins; C041348:tcdA protein, Clostridium difficile; C031815:toxB protein, Clostridium difficile