Further development of a recombinant feline herpesvirus type 1 expressing the Gag protein of feline immunodeficiency virus.
We have previously reported the construction of a recombinant feline herpesvirus type 1 (FHV-1), designated C7301ddlTK-gag, expressing the Gag precursor protein of feline immunodeficiency virus (FIV). In this study, we report the construction of a further recombinant FHV-1 (ddlTK(gBp)-gag) which carries an FHV-1 gB promoter sequence upstream of the FIV gag gene of C7301ddlTK-gag. Strong expression of the FIV Gag protein by ddlTK(gBp)-gag was confirmed by immunoblot analyses and enzyme-linked immunosorbent assays. Although C7301ddlTK-gag and ddlTK(gBp)-gag failed to induce anti-FIV Gag antibodies in cats, we confirmed the infectivity and stability of these recombinants in cats.
Sato
E
E
Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
Miyazawa
T
T
Nishimura
Y
Y
Ikeda
Y
Y
Kawakami
K
K
Mochizuki
M
M
Yokoyama
N
N
Maeda
K
K
Fujita
K
K
Kohmoto
M
M
Takahashi
E
E
Mikami
T
T
D000818:Animals; D002371:Cat Diseases / Q000276:immunology* / Q000821:virology; D002415:Cats; D002460:Cell Line; D016181:Feline Acquired Immunodeficiency Syndrome / Q000276:immunology; D015683:Gene Products, gag / Q000235:genetics*; D015750:Genes, gag; D006566:Herpesviridae Infections / Q000276:immunology / Q000662:veterinary*; D016089:Immunodeficiency Virus, Feline / Q000201:enzymology / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D011995:Recombination, Genetic; D015183:Restriction Mapping; D013937:Thymidine Kinase / Q000235:genetics; D018140:Varicellovirus / Q000235:genetics* / Q000276:immunology
D015683:Gene Products, gag; D013937:Thymidine Kinase