title: Helicobacter pylori vacA, iceA, and cagA status and pattern of gastritis in patients with malignant and benign gastroduodenal disease.
abstract: OBJECTIVE
  Both bacterial virulence factors and the pattern of Helicobacter pylori (H. pylori) gastritis may contribute to the development of clinically relevant gastroduodenal disease. The aim of our study was to investigate the frequency of H. pylori vacA alleles, iceA, and cagA, and the pattern of gastritis in patients with gastric cancer (GC), gastric lymphoma (MALT), duodenal ulcer (DU), and functional dyspepsia (FD).
  
  
  METHODS
  H. pylori was cultured from 141 patients (34 GC, 26 MALT, 49 DU, 32 FD). Allelic variants of vacA and iceA, and cagA were identified by polymerase chain reaction. Antrum and corpus biopsies were obtained for assessment of gastritis according to the updated Sydney System.
  
  
  RESULTS
  The vacA sl,ml genotype was more frequently detected in H. pylori from GC patients (70.6%) than from MALT, DU, and FD patients (p < 0.05). The frequency of iceA1 and cagA did not differ among the groups. The proportion of patients with severe gastritis in the corpus was significantly higher in patients with GC and MALT compared with patients with DU (p < 0.001).
  
  
  CONCLUSIONS
  In a German patient population, only the vacA s1,m1 genotype of H. pylori is associated with GC, and therefore may be useful to identify infected patients being at an increased risk for GC.
authors.affiliation: Medical Department I and Institute of Medical Microbiology, Technical University Hospital, Dresden, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Miehlke
authors.forename: J
authors.initials: J
authors.lastname: Yu
authors.forename: M
authors.initials: M
authors.lastname: Schuppler
authors.forename: C
authors.initials: C
authors.lastname: Frings
authors.forename: C
authors.initials: C
authors.lastname: Kirsch
authors.forename: N
authors.initials: N
authors.lastname: Negraszus
authors.forename: A
authors.initials: A
authors.lastname: Morgner
authors.forename: M
authors.initials: M
authors.lastname: Stolte
authors.forename: G
authors.initials: G
authors.lastname: Ehninger
authors.forename: E
authors.initials: E
authors.lastname: Bayerdörffer
mesh_terms: D000368:Aged; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis*; D001426:Bacterial Proteins / Q000032:analysis*; D004381:Duodenal Ulcer / Q000382:microbiology*; D004415:Dyspepsia / Q000382:microbiology*; D005260:Female; D005756:Gastritis / Q000382:microbiology*; D005838:Genotype; D016481:Helicobacter Infections / Q000150:complications*; D016480:Helicobacter pylori / Q000235:genetics*; D006801:Humans; D018442:Lymphoma, B-Cell, Marginal Zone / Q000382:microbiology*; D008297:Male; D013274:Stomach Neoplasms / Q000382:microbiology*
chemical_list: D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; C074689:VacA protein, Helicobacter pylori; C080866:cagA protein, Helicobacter pylori; C063113:ice nucleation protein