title: Stimulation of human neutrophil leukocyte aerobic glucose metabolism by purified chemotactic factors.
abstract: The interaction of human neutrophils adherent to plastic petri dishes with the purified chemotactic factors C5a and kallikrein increased their rate of aerobic glycolysis 25-120% and the activity of their hexose monophosphate shunt (HMPS) 100-600%, reaching a plateau after 2 hr at 37 degrees C. The stimulation of either pathway required a chemotactically active stimulus since neither C5 nor prekallikrein or inactivated kallikrein could enhance metabolic activity. Marked suppression of the neutrophil chemotactic response by preincubation with a chemotactic factor to achieve deactivation, 5 x 10(-7) M diisopropyl fluorophosphate, or the neutrophil immobilizing factor (NIF) did not prevent the stimulation of HMPS activity or glycolysis by chemotactic factors. The metabolic inhibitors iodoacetate and 6-aminonicotinamide at concentrations which blocked enhancement of glycolysis or HMPS activity, respectively, partially suppressed the chemotactic response of neutrophils to the chemotactic factors. The capacity of a chemotactic factor to stimulate glucose metabolism of human neutrophils is associated with a maximal chemotactic response, but this stimulation is not alone sufficient for chemotaxis.
authors.affiliation: Department of Medicine, Harvard Medical School and Robert B. Brigham Hospital, Boston, Massachusetts 02120, USA.
authors.forename: E J
authors.initials: EJ
authors.lastname: Goetzl
authors.forename: K F
authors.initials: KF
authors.lastname: Austen
mesh_terms: D000332:Aerobiosis; D002478:Cells, Cultured; D002630:Chemotactic Factors / Q000378:metabolism* / Q000494:pharmacology; D015936:Complement C5a / Q000378:metabolism* / Q000494:pharmacology; D005459:Fluorides / Q000378:metabolism / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism*; D006019:Glycolysis; D006801:Humans; D007610:Kallikreins / Q000378:metabolism* / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D010427:Pentose Phosphate Pathway; D010710:Phosphates / Q000378:metabolism / Q000494:pharmacology
chemical_list: D002630:Chemotactic Factors; D010710:Phosphates; C012980:fluorophosphate; D015936:Complement C5a; D007610:Kallikreins; D005947:Glucose; D005459:Fluorides