title: Antitumor immunity induced by irradiated tumor cells producing macrophage colony-stimulating factor.
abstract: We previously reported that administration into mice of mouse lymphoid leukemia L1210 cells engineered to secrete macrophage colony-stimulating factor (M-CSF) could lead to tumor rejection. Here, we demonstrate that inoculation with irradiated M-CSF-producing cells protects mice against a subsequent challenge with unmodified parental tumor cells. We used 2 experimental protocols: the inoculation with irradiated M-CSF-producing L1210 cells (EM5) before the challenge with parental cells and after the challenge with parental cells. Both protocols effectively improved the survival rate of mice compared with protocols in which irradiated non-M-CSF-producing L1210 cells (EM-mock) were inoculated. Inoculation with 1 x 10(2) irradiated EM5 cells was sufficient to prolong the survival time of mice subsequently challenged with 1 x 10(4) parental cells. In vivo depletion experiments with administration of antibodies suggested the involvement of CD4+ T cells, CD8+ T cells, and natural killer (NK) cells in the antitumor effect. Consistent with these findings, the cytotoxic T lymphocyte activity of splenocytes from EM5-inoculated mice was higher than that from EM-mock-inoculated mice, and L1210 tumors were heavily infiltrated by CD4+ T cells and NK cells as well as macrophages in EM5-inoculated mice.
authors.affiliation: Biochemical Research Laboratory, Morinaga Milk Industry, Kanagawa, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Suzu
authors.forename: F
authors.initials: F
authors.lastname: Kimura
authors.forename: M
authors.initials: M
authors.lastname: Tanaka-Douzono
authors.forename: M
authors.initials: M
authors.lastname: Yamada
authors.forename: Y
authors.initials: Y
authors.lastname: Nakamura
authors.forename: N
authors.initials: N
authors.lastname: Wakimoto
authors.forename: K
authors.initials: K
authors.lastname: Sato
authors.forename: T
authors.initials: T
authors.lastname: Morita
authors.forename: K
authors.initials: K
authors.lastname: Ikeda
authors.forename: K
authors.initials: K
authors.lastname: Motoyoshi
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D019496:Cancer Vaccines; D006084:Graft Rejection; D007694:Killer Cells, Natural / Q000276:immunology; D007939:Leukemia L1210 / Q000276:immunology / Q000517:prevention & control*; D016246:Lymphocytes, Tumor-Infiltrating / Q000276:immunology; D016173:Macrophage Colony-Stimulating Factor / Q000235:genetics* / Q000502:physiology; D008264:Macrophages / Q000276:immunology; D051379:Mice; D009368:Neoplasm Transplantation; D014411:Neoplastic Stem Cells / Q000276:immunology / Q000528:radiation effects / Q000637:transplantation*; D011993:Recombinant Fusion Proteins / Q000502:physiology; D013154:Spleen / Q000473:pathology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014162:Transfection; D014611:Vaccination
chemical_list: D019496:Cancer Vaccines; D011993:Recombinant Fusion Proteins; D016173:Macrophage Colony-Stimulating Factor