Binding of tenascin-X to decorin.
Tenascin-X (TN-X) is an extracellular matrix protein whose absence results in an alteration of the mechanical properties of connective tissue. To understand the mechanisms of integration of TN-X in the extracellular matrix, overlay blot assays were performed on skin extracts. A 100 kDa molecule interacting with TN-X was identified by this method and this interaction was abolished when the extract was digested by chondroitinase. By solid-phase assays, we showed that dermatan sulfate chains of decorin bind to the heparin-binding site included within the fibronectin-type III domains 10 and 11 of TN-X. We thus postulate that the association of TN-X with collagen fibrils is mediated by decorin and contributes to the integrity of the extracellular network.
Elefteriou
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F
Institut de Biologie et Chimie des Protéines, CNRS UMR 5086, Université Claude Bernard, 7 passage du Vercors, 69367 Cedex 07, Lyon, France.
Exposito
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JY
Garrone
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R
Lethias
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C
D000818:Animals; D058578:Biglycan; D001665:Binding Sites / Q000187:drug effects / Q000502:physiology; D001667:Binding, Competitive / Q000187:drug effects; D015153:Blotting, Western; D002417:Cattle; D019765:Chondroitin ABC Lyase / Q000378:metabolism; D003094:Collagen / Q000378:metabolism; D058575:Decorin; D004305:Dose-Response Relationship, Drug; D016326:Extracellular Matrix Proteins; D006025:Glycosaminoglycans / Q000378:metabolism / Q000494:pharmacology; D006493:Heparin / Q000378:metabolism; D011485:Protein Binding / Q000187:drug effects / Q000502:physiology; D017434:Protein Structure, Tertiary / Q000502:physiology; D011509:Proteoglycans / Q000378:metabolism* / Q000494:pharmacology; D012867:Skin / Q000737:chemistry / Q000196:embryology; D019063:Tenascin / Q000737:chemistry* / Q000378:metabolism*; D014020:Tissue Extracts / Q000737:chemistry
D058578:Biglycan; D058575:Decorin; D016326:Extracellular Matrix Proteins; D006025:Glycosaminoglycans; D011509:Proteoglycans; D019063:Tenascin; D014020:Tissue Extracts; C081865:tenascin X; D006493:Heparin; D003094:Collagen; D019765:Chondroitin ABC Lyase