title: Sequencing and analysis of the Edwardsiella ictaluri plasmids.
abstract: To determine possible functions of the Edwardsiella ictaluri plasmids, pEI1 and pEI2, we analyzed the sequence of both plasmids. Plasmid pEI1 is 4807 bp, with 51% G + C, and 23 possible open reading frames of 40 amino acids or greater. Plasmid pEI2 is 5643 bp, with 51% G + C, and 24 possible reading frames. Database searches indicated that pEI1 contains an insertion element and a ROM analog. In addition, pEI1 possesses an open reading frame with strong homology to SlrP, SspH1, and SspH2 of Salmonella typhimurium and IpaH of Shigella flexnari, which have leucine-rich repeat regions and are components of type III secretory systems. pEI2 has a frame with weak homology to Spa15 of S. flexnari 5 and InvB of S. sonnei and S. typhimurium, which are also type III secretory system components, three origins of replication, a Rep analog, and a multimer resolution site.
authors.affiliation: Department of Veterinary Science, Louisiana State University Agricultural Center, Baton Rouge 70803, USA.
authors.forename: D H
authors.initials: DH
authors.lastname: Fernandez
authors.forename: L
authors.initials: L
authors.lastname: Pittman-Cooley
authors.forename: R L
authors.initials: RL
authors.lastname: Thune
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics*; D004251:DNA Transposable Elements / Q000235:genetics; D020610:Edwardsiella ictaluri / Q000235:genetics*; D000090263:Leucine-Rich Repeat Proteins; D016366:Open Reading Frames; D010957:Plasmids / Q000235:genetics*; D011506:Proteins / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D000090263:Leucine-Rich Repeat Proteins; D011506:Proteins; C042833:ROM protein, Bacteria