Rhizobium sp. BR816 produces a complex mixture of known and novel lipochitooligosaccharide molecules.
Rhizobial lipochitooligosaccharide (LCO) signal molecules induce various plant responses, leading to nodule development. We report here the LCO structures of the broadhost range strain Rhizobium sp. BR816. The LCOs produced are all pentamers, carrying common C18:1 or C18:0 fatty acyl chains, N-methylated and C-6 carbamoylated on the nonreducing terminal N-acetylglucosamine and sulfated on the reducing/terminal residue. A second acetyl group can be present on the penultimate N-acetylglucosamine from the nonreducing terminus. Two novel characteristics were observed: the reducing/terminal residue can be a glucosaminitol (open structure) and the degree of acetylation of this glucosaminitol or of the reducing residue can vary.
Snoeck
C
C
Centre of Microbial and Plant Genetics, Heverlee, Belgium.
Luyten
E
E
Poinsot
V
V
Savagnac
A
A
Vanderleyden
J
J
Promé
J C
JC
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D007887:Fabaceae / Q000382:microbiology; D008070:Lipopolysaccharides / Q000096:biosynthesis* / Q000737:chemistry* / Q000302:isolation & purification; D013058:Mass Spectrometry; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry; D010946:Plants, Medicinal; D012231:Rhizobium / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization; D018629:Spectrometry, Mass, Secondary Ion
D008070:Lipopolysaccharides; D009844:Oligosaccharides; C023023:lipid-linked oligosaccharides