Delivery to macrophages of interleukin 3 loaded in mouse erythrocytes.
Mouse carrier erythrocytes containing 125I-interleukin 3 have been prepared and treated with band 3 crosslinking reagents. The incorporation of interleukin 3 by hypotonic treatment into mouse erythrocytes reached levels of about 15% of the interleukin 3 added to the medium being predominantly present in the cytosolic fraction (73%). Uptake fell to about 7.4% when using the same conditions but omitting hypotonic shock. The interaction of band 3 crosslinked interleukin 3 loaded erythrocytes with macrophages was also studied. A high level of incorporation of interleukin 3 into macrophages was observed either from band 3 crosslinked, interleukin 3-loaded erythrocytes or from interleukin 3 loaded erythrocytes. The observations encourage the view that the system may be able to deliver and target cytokines and other growth factors to macrophages.
Olmos
G
G
Departamento de Bioquímica y Biología Molecular, Universidad de Alcalá, Madrid, Spain.
Lotero
L A
LA
Tejedor
M C
MC
Diez
J C
JC
D000818:Animals; D001457:Anion Exchange Protein 1, Erythrocyte / Q000737:chemistry / Q000378:metabolism; D002478:Cells, Cultured; D003432:Cross-Linking Reagents / Q000737:chemistry; D016503:Drug Delivery Systems; D004912:Erythrocytes / Q000378:metabolism*; D007377:Interleukin-3 / Q000493:pharmacokinetics*; D007457:Iodine Radioisotopes; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008815:Mice, Inbred Strains; D013388:Succinimides / Q000737:chemistry
D001457:Anion Exchange Protein 1, Erythrocyte; D003432:Cross-Linking Reagents; D007377:Interleukin-3; D007457:Iodine Radioisotopes; D013388:Succinimides; C035760:bis(sulfosuccinimidyl)suberate