title: The P450-1 gene of Gibberella fujikuroi encodes a multifunctional enzyme in gibberellin biosynthesis.
abstract: Recent studies have shown that the genes of the gibberellin (GA) biosynthesis pathway in the fungus Gibberella fujikuroi are organized in a cluster of at least seven genes. P450-1 is one of four cytochrome P450 monooxygenase genes in this cluster. Disruption of the P450-1 gene in the GA-producing wild-type strain IMI 58289 led to total loss of GA production. Analysis of the P450-1-disrupted mutants indicated that GA biosynthesis was blocked immediately after ent-kaurenoic acid. The function of the P450-1 gene product was investigated further by inserting the gene into mutants of G. fujikuroi that lack the entire GA gene cluster; the gene was highly expressed under GA production conditions in the absence of the other GA-biosynthesis genes. Cultures of transformants containing P450-1 converted ent-[(14)C]kaurenoic acid efficiently into [(14)C]GA(14), indicating that P450-1 catalyzes four sequential steps in the GA-biosynthetic pathway: 7beta-hydroxylation, contraction of ring B by oxidation at C-6, 3beta-hydroxylation, and oxidation at C-7. The GA precursors ent-7alpha-hydroxy[(14)C]kaurenoic acid, [(14)C]GA(12)-aldehyde, and [(14)C]GA(12) were also converted to [(14)C]GA(14). In addition, there is an indication that P450-1 may also be involved in the formation of the kaurenolides and fujenoic acids, which are by-products of GA biosynthesis in G. fujikuroi. Thus, P450-1 displays remarkable multifunctionality and may be responsible for the formation of 12 products.
authors.affiliation: Laboratorio de Bioorgánica, Departamento de Quimica, Facultad de Ciencias, Universidad de Chile, Casilla 653, Santiago, Chile.
authors.forename: M C
authors.initials: MC
authors.lastname: Rojas
authors.forename: P
authors.initials: P
authors.lastname: Hedden
authors.forename: P
authors.initials: P
authors.lastname: Gaskin
authors.forename: B
authors.initials: B
authors.lastname: Tudzynski
mesh_terms: D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D017931:DNA Primers; D005874:Gibberella / Q000201:enzymology / Q000235:genetics*; D005875:Gibberellins / Q000096:biosynthesis*; D016133:Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D005875:Gibberellins; D003577:Cytochrome P-450 Enzyme System