Inducible protective processes in animal systems: VIII. Enhancement of adaptive response by nicotinamide.
The molecular mechanism of the adaptive response or inducible DNA repair process has not been clearly demonstrated in eukaryotic systems. The involvement of poly(ADP-ribose) polymerase (PARP), a DNA repair enzyme has been reported in the adaptive response (Shadley and Wolff, 1987; Wiencke, 1987). Hence, the present studies were undertaken to understand the role of PARP in ethyl methanesulfonate (EMS)-induced adaptive response in mouse bone marrow cells by employing the inhibitor of this enzyme, nicotinamide. Inter-, pre- and post-treatments of nicotinamide with EMS were made. The results have revealed that there is a reduction in the frequencies of chromosomal aberrations compared with combined or challenge treatment at the different recovery times tested. These results are discussed with reference to the enhancement of the adaptive response by nicotinamide in mouse bone marrow cells.
Guruprasad
K P
KP
Department of Zoology, Manasagangotri, University of Mysore, Mysore, 570 006 India.
Vasudev
V
V
D000222:Adaptation, Physiological / Q000187:drug effects*; D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects; D002869:Chromosome Aberrations; D004247:DNA / Q000187:drug effects; D004260:DNA Repair; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005020:Ethyl Methanesulfonate; D008297:Male; D051379:Mice; D009153:Mutagens; D009536:Niacinamide / Q000494:pharmacology*; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D013997:Time Factors
D004791:Enzyme Inhibitors; D009153:Mutagens; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D009536:Niacinamide; D004247:DNA; D005020:Ethyl Methanesulfonate; D011065:Poly(ADP-ribose) Polymerases