High-performance liquid chromatography-electrospray ionization mass spectrometry method for the measurement of moclobemide and two metabolites in plasma.
A rapid and sensitive liquid chromatography-electrospray ionisation mass spectrometry (HPLC-ESI-MS) assay has been developed for the measurement of moclobemide and metabolites, Ro12-5637 and Ro12-8095, in human plasma. Sample preparation (0.5 ml plasma) involves solid-phase extraction using C18 cartridges. A Nova-Pak phenyl column (Waters, 4 microm, 150x2 mm I.D.) was employed for analyte separation with a mixture of 0.2 M ammonium formate buffer, pH 3.57 and acetonitrile as the mobile phase. The within- and between-day precisions of the assay were <18% and the limit of quantification for all analytes was 0.01 microg/ml. The total run-time was 6 min. The method described was used to measure moclobemide, Ro12-5637 and Ro12-8095 in human plasma following an oral 300 mg dose.
Hoskins
J M
JM
Department of Clinical Pharmacology, Royal North Shore Hospital, St. Leonards, NSW, Australia.
Gross
A S
AS
Shenfield
G M
GM
Rivory
L P
LP
D000928:Antidepressive Agents / Q000097:blood / Q000378:metabolism; D001549:Benzamides / Q000097:blood; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D006801:Humans; D020912:Moclobemide / Q000097:blood* / Q000378:metabolism; D009025:Morpholines / Q000097:blood; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D000928:Antidepressive Agents; D001549:Benzamides; D009025:Morpholines; C062936:Ro 12-5637; C062937:Ro 12-8095; D020912:Moclobemide