Reduced adhesion of monocyte-derived macrophages from CD36-deficient patients to type I collagen.
CD36 is an 88-kDa glycoprotein expressed on platelets and monocyte/macrophages (Mphi). CD36 is a multifunctional receptor for collagen, thrombospondin, oxidized low density lipoproteins (LDL), and long-chain fatty acids. The present study was performed to investigate whether CD36 can function as an adhesion molecule which is involved in mediating human macrophages (Mphi) adhesion to type I collagen in vitro. The Mphi of human CD36-deficient as well as normal control subjects were isolated and cultured on the multi-well plates coated with type I collagen, a natural ligand for CD36. Up to 2 h of incubation, the Mphi from CD36-deficient patients showed almost a approximately 55% decrease in adhesion to type I collagen in comparison to those from controls (P < 0.01). However, there was no significant difference in the adhesion thereafter. Furthermore, the addition of antibody against CD36 into the media of control Mphi significantly inhibited the adhesion by approximately 50% (P < 0.05). The addition of oxidized LDL (OxLDL) did not alter adhesion of Mphi from both CD36-deficient and controls. These data suggest that CD36 is involved in the adhesion of Mphi to type I collagen, especially in the early stage of adhesion.
Janabi
M
M
Department of Internal Medicine and Molecular Science, Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita, Osaka, 565-0871, Japan.
Yamashita
S
S
Hirano
K
K
Matsumoto
K
K
Sakai
N
N
Hiraoka
H
H
Kashiwagi
H
H
Tomiyama
Y
Y
Nozaki
S
S
Matsuzawa
Y
Y
D000911:Antibodies, Monoclonal / Q000494:pharmacology; D000918:Antibody Specificity; D016824:Antigens, Human Platelet / Q000235:genetics; D018955:CD36 Antigens / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D002448:Cell Adhesion / Q000187:drug effects / Q000235:genetics; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008077:Lipoproteins, LDL / Q000378:metabolism / Q000494:pharmacology; D008264:Macrophages / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D009000:Monocytes / Q000166:cytology / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D016824:Antigens, Human Platelet; D018955:CD36 Antigens; D008077:Lipoproteins, LDL; C062601:Naka isoantigen, human; C099252:oxidized low density lipoprotein; D003094:Collagen