Changes in the structure of xyloglucan during cell elongation.
Xyloglucans were isolated by sequential extraction of the cell walls of pea (Pisum sativum L. cv. Alaska) with a xyloglucan-specific endoglucanase and KOH. The xyloglucan content and xyloglucan-oligosaccharide composition were determined for fractions obtained from the elongating and non-elongating segments of pea stems grown in the light and in darkness. The results were consistent with the hypothesis that regulated growth of the cell wall depends on xyloglucan metabolism. Furthermore, the characterization of xyloglucan extracted from leaves of light-grown pea plants indicates that xyloglucan metabolism is tissue specific. Changes in xyloglucan subunit structure observed in elongating stems are consistent with the in muro realization of a metabolic pathway that was previously proposed solely on the basis of the in vitro activities of plant glycosyl hydrolases.
Pauly
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Complex Carbohydrate Research Center and Department of Biochemistry and Molecular Biology, University of Georgia, 220 Riverbend Road, Athens, GA 30602-4712, USA.
Qin
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Greene
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Albersheim
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Darvill
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York
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D015084:2,4-Dichlorophenoxyacetic Acid / Q000494:pharmacology; D001681:Biological Assay; D002240:Carbohydrate Sequence; D002455:Cell Division; D002473:Cell Wall / Q000737:chemistry / Q000378:metabolism; D003624:Darkness; D005936:Glucans; D006026:Glycoside Hydrolases / Q000378:metabolism; D006540:Herbicides / Q000494:pharmacology; D006878:Hydroxides / Q000494:pharmacology; D006898:Hydroxylamines; D008027:Light; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry; D018532:Pisum sativum / Q000254:growth & development / Q000378:metabolism*; D018515:Plant Leaves / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism; D018547:Plant Stems / Q000737:chemistry / Q000254:growth & development; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D017680:Potassium Compounds / Q000494:pharmacology; D014990:Xylans
D005936:Glucans; D006540:Herbicides; D006878:Hydroxides; D006898:Hydroxylamines; D009844:Oligosaccharides; D011134:Polysaccharides; D017680:Potassium Compounds; D014990:Xylans; C037705:4-nitrobenzyloxyamine; D015084:2,4-Dichlorophenoxyacetic Acid; C029353:xyloglucan; D006026:Glycoside Hydrolases; C072818:xyloglucan endo(1-4)-beta-D-glucanase; C029943:potassium hydroxide