title: Purification and characterization of the enantioselective nitrile hydratase from Rhodococcus equi A4.
abstract: The nitrile hydratase from Rhodococcus equi A4 consisted of two kinds of subunits which slightly differed in molecular weight (both approximately 25 kDa) and showed a significant similarity in the N-terminal amino acid sequences to those of the nitrile hydratase from Rhodococcus sp. N-774. The enzyme preferentially hydrated the S-isomers of racemic 2-(2-, 4-methoxyphenyl)propionitrile, 2-(4-chlorophenyl)propionitrile and 2-(6-methoxynaphthyl)propionitrile (naproxennitrile) with E-values of 5-15. The enzyme functioned in the presence of 5-98% (v/v) of different hydrocarbons, alcohols or diisopropyl ether. The addition of 5% (v/v) of n-hexane, n-heptane, isooctane, n-hexadecane, pristane and methanol increased the E-value for the enzymatic hydration of 2-(6-methoxynaphthyl)propionitrile.
authors.affiliation: Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague.
authors.forename: I
authors.initials: I
authors.lastname: Prepechalová
authors.forename: L
authors.initials: L
authors.lastname: Martínková
authors.forename: A
authors.initials: A
authors.lastname: Stolz
authors.forename: M
authors.initials: M
authors.lastname: Ovesná
authors.forename: K
authors.initials: K
authors.lastname: Bezouska
authors.forename: J
authors.initials: J
authors.lastname: Kopecký
authors.forename: V
authors.initials: V
authors.lastname: Kren
mesh_terms: D000595:Amino Acid Sequence; D006836:Hydro-Lyases / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D006838:Hydrocarbons / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007526:Isoelectric Point; D000432:Methanol / Q000494:pharmacology; D008970:Molecular Weight; D009570:Nitriles / Q000378:metabolism*; D021122:Protein Subunits; D016929:Rhodococcus equi / Q000201:enzymology*; D012997:Solvents / Q000494:pharmacology; D013237:Stereoisomerism; D013379:Substrate Specificity; D013696:Temperature
chemical_list: D006838:Hydrocarbons; D009570:Nitriles; D021122:Protein Subunits; D012997:Solvents; D006836:Hydro-Lyases; C048831:nitrile hydratase; D000432:Methanol