title: Indirect neurotrophic effect of neuropeptide ZNC(C)PR on PC12 cells via peptide-stimulation of C6 cells.
abstract: OBJECTIVE
  To understand the mechanism of neurotrophic action of neuropeptide ZNC(C)PR and its effect on which could affect both growth and apoptosis of C6 cells.
  
  
  METHODS
  Effects of ZNC(C)PR-treated C6 conditioned medium was observed on on culture of PC12 cells. The development of PC12 cells was determined by ratio of neurite-bearing cells in the total cells. The specific binding of ZNC(C)PR on C6 cells was determined by radioligand binding assay (RBA).
  
  
  RESULTS
  ZNC(C)PR-treated C6 conditioned medium increased the ratio of neurite-bearing PC12 cells by 36% compared to the untreated C6 conditioned medium or to a mixture of ZNC(C)PR with the untreated C6 conditioned medium. RBA showed a specific binding site of ZNC(C)PR on C6 cells with Kd value of 2.74 nmol.L-1 and Bmax value of 19 pmol.g-1 protein.
  
  
  CONCLUSIONS
  ZNC(C)PR enhanced C6 cells induced secretion of some neurotrophic factors which acted as enhancers for PC12 cells differentiation, through its specific receptor sites on the neuroglioma cell.
authors.affiliation: Shanghai Institute of Biochemistry, Chinese Academy of Sciences, Shanghai 200031, China.
authors.forename: Q W
authors.initials: QW
authors.lastname: Yan
authors.forename: X G
authors.initials: XG
authors.lastname: Zhen
authors.forename: T X
authors.initials: TX
authors.lastname: Wang
authors.forename: K Y
authors.initials: KY
authors.lastname: Xu
authors.forename: M
authors.initials: M
authors.lastname: Dong
authors.forename: Y C
authors.initials: YC
authors.lastname: Du
mesh_terms: D000818:Animals; D001127:Arginine Vasopressin / Q000494:pharmacology*; D001665:Binding Sites; D001932:Brain Neoplasms / Q000473:pathology; D002454:Cell Differentiation; D002455:Cell Division; D002462:Cell Membrane; D017077:Culture Media, Conditioned; D005910:Glioma / Q000473:pathology; D009414:Nerve Growth Factors / Q000494:pharmacology*; D016716:PC12 Cells; D010446:Peptide Fragments / Q000494:pharmacology*; D051381:Rats; D014407:Tumor Cells, Cultured
chemical_list: C094362:(phenylmethoxy)carbonyl-asparaginyl-(cysteinyl)cysteinyl-prolyl-arginine; D017077:Culture Media, Conditioned; D009414:Nerve Growth Factors; D010446:Peptide Fragments; D001127:Arginine Vasopressin