Molecular cloning and complete cDNA sequences of the ribosomal proteins rpl34 and rpl44 from Aedes triseriatus mosquitoes.
We present the complete cDNA and deduced amino acid sequences of the 60S ribosomal subunit proteins, rpL34 and rpL44, from Aedes triseriatus mosquitoes. The rpL34 cDNA is 554 nucleotides in length and encodes a 139 amino acid protein with a calculated molecular mass of 15 732 daltons. The putative protein displays strong sequence similarity to rpL34 of Aedes albopictus mosquitoes (92%), humans (60%) and rats (58%). The protein is highly basic and contains a C-terminal repetitive-alanine domain and four putative nucleolar localization signals. The rpL44 cDNA consists of 450 nucleotides and encodes a 104 amino acid protein with a calculated molecular mass of 12 544 daltons. The putative protein displays strong sequence similarity to rpL44 of Brugia malayi (87%), Caenorhabditis elegans (86%) and humans (85%). The protein is highly basic and contains a putative nucleolar localization signal. The mRNAs for both rpL34 and rpL44 were detected in biosynthetically active (embryonating) and dormant (diapausing) Ae. triseriatus embryos by RT-PCR analysis.
Blitvich
B J
BJ
Arthropod-Borne and Infectious Diseases Laboratory, Department of Microbiology, Colorado State University, Fort Collins, Colorado 80523, USA. blitvich@cvmbs.colostate.edu
Rayms-Keller
A
A
Blair
C D
CD
Beaty
B J
BJ
D000330:Aedes / Q000196:embryology / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D004247:DNA; D019476:Insect Proteins / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012269:Ribosomal Proteins / Q000235:genetics*; D017385:Sequence Homology
D019476:Insect Proteins; D012269:Ribosomal Proteins; C029466:ribosomal protein L2; C050985:ribosomal protein L31; D004247:DNA