New molecular markers for phlebotomine sand flies.
Using degenerate-primers PCR we isolated and sequenced fragments from the sand fly Lutzomyia longipalpis homologous to two behavioural genes in Drosophila, cacophony and period. In addition we identified a number of other gene fragments that show homology to genes previously cloned in Drosophila. A codon usage table for L. longipalpis based on these and other genes was calculated. These new molecular markers will be useful in population genetics and evolutionary studies in phlebotomine sand flies and in establishing a preliminary genetic map in these important leishmaniasis vectors.
Peixoto
A A
AA
Department of Biochemistry and Molecular Biology, Fundação Oswaldo Cruz, Av. Brasil 4365--Manguinhos, CEP 21045-900, Rio de Janeiro, Brazil. apeixoto@gene.dbbm.fiocruz.br
Gomes
C A
CA
de Amoretty
P R
PR
Lins
R M
RM
Meireles-Filho
A C
AC
de Souza
N A
NA
Kyriacou
C P
CP
D000595:Amino Acid Sequence; D000818:Animals; D003062:Codon / Q000737:chemistry; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D004330:Drosophila / Q000235:genetics; D005819:Genetic Markers / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011576:Psychodidae / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012726:Sexual Behavior, Animal / Q000502:physiology
D003062:Codon; D005819:Genetic Markers; D004247:DNA