Hyaluronan reduces migration and proliferation in CHO cells.
Expression of the hyaluronan synthase gene in hyaluronan-deficient CHO cells changed the cell morphology from a spindle shape to a flattened epithelial-type form. Hyaluronan producing CHO cells showed reduced initial cell adhesion, migration, proliferation and density at contact inhibition, but no difference in random migration determined by the Boyden chamber assay. Addition of hyaluronan to the medium of CHO cells reduced migration, proliferation and initial cell adhesion. In contrast, coating the plastic dish with hyaluronan enhanced initial cell adhesion. These results are discussed in the context of the perplexing properties of hyaluronan on cellular functions.
Dübe
B
B
Institut für Physiologische Chemie und Pathobiochemie, Münster, Germany.
Lüke
H J
HJ
Aumailley
M
M
Prehm
P
P
D000818:Animals; D016466:CHO Cells / Q000378:metabolism*; D002448:Cell Adhesion / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D048429:Cell Size / Q000187:drug effects; D006224:Cricetinae; D014453:Glucuronosyltransferase / Q000096:biosynthesis / Q000235:genetics; D016695:Glycosyltransferases; D000076002:Hyaluronan Synthases; D006820:Hyaluronic Acid / Q000096:biosynthesis* / Q000172:deficiency / Q000494:pharmacology; D008565:Membrane Proteins; D014162:Transfection; D014166:Transferases; D029867:Xenopus Proteins
D008565:Membrane Proteins; D029867:Xenopus Proteins; D006820:Hyaluronic Acid; D014166:Transferases; D016695:Glycosyltransferases; D014453:Glucuronosyltransferase; C056944:HAS1 protein, Xenopus; D000076002:Hyaluronan Synthases