[Protective effect of schisanhenol (SAL) on oxygen free radical induced injury of spleen lymphocytes in mice].
In the present paper the effects of Sal, an active component isolated from Kadsura Longipenduculata Finet et Gagnep, on oxygen free radical induced injury of murine spleen lymphocytes in vitro and in vivo were investigated. The results showed that Sal at 1 x 10(-4) mol.L-1 significantly inhibited the decrease of GSH content induced by Fe(2+)-Vit C and the increase of MDA formation induced by Fe(2+)-Cys in murine spleen lymphocytes. The decrease of membrane fluidity of lymphocytes under lipid peroxidation induced by Fe(2+)-Vit C was prevented by Sal. The damage of the lymphocyte surfaces induced by Fe(2+)-Vit C was also markedly reduced by Sal. Administration of Sal 20 mg.kg-1.d-1 for 8 d significantly increased the GSH content of the spleen lymphocytes in mice under high oxygen-stress. Meanwhile, the compensative increase of SOD activity in the spleen lymphocytes was reversed. The results suggest that Sal may protect murine spleen lymphocytes from the injury induced by oxygen free radicals.
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Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050.
Wu
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D000818:Animals; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology; D002462:Cell Membrane / Q000187:drug effects; D034242:Cyclooctanes; D005260:Female; D005609:Free Radicals; D005978:Glutathione / Q000097:blood*; D006931:Hyperbaric Oxygenation; D015227:Lipid Peroxidation / Q000187:drug effects; D008214:Lymphocytes / Q000473:pathology*; D008315:Malondialdehyde / Q000097:blood; D008560:Membrane Fluidity / Q000187:drug effects; D051379:Mice; D011083:Polycyclic Compounds / Q000378:metabolism / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology
D000975:Antioxidants; D034242:Cyclooctanes; D005609:Free Radicals; D011083:Polycyclic Compounds; D008315:Malondialdehyde; C045309:schisanhenol; D005978:Glutathione