[Study of protein separation by reversed-phase high performance liquid chromatography].
Reversed-phase high performance liquid chromatography (RP-HPLC) of protein on large pore silica (20-30 nm) n-alkyl-bonded(C4 and C5) phase columns was studied using a trifluoroacetic acid (TFA)/2-propanol mobile phase system. The influence of concentration of organic modifier (2-propanol) and ion-pairing acid (TFA) on the protein retention time was examined. The results showed that the plot of the retention time vs-2-propanol concentration in mobile phase was U-shaped. The mechanism of protein in reversed-phase high performance liquid chromatography is also discussed.
Zhang
H
H
State Key Laboratory of Dye and Surfactant Fine Chemicals, Dalian University of Technology, Dalian, 116012.
Wang
J
J
Zhong
H
H
Luo
L
L
D000510:Alpha-Globulins / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D007328:Insulin / Q000302:isolation & purification*; D009113:Muramidase / Q000302:isolation & purification*
D000510:Alpha-Globulins; D007328:Insulin; D009113:Muramidase