Photochemiluminescent detection of antiradical activity. V. Application in combination with the hydrogen peroxide-initiated chemiluminescence of blood plasma proteins to evaluate antioxidant homeostasis in humans.
Disturbance of the steady state between pro- and antioxidants in tissues is an important aetiopathogenetic factor. Two method--(i) photosensitized chemiluminescence for detection of antiradical activity and (ii) hydrogen peroxide-initiated chemiluminescence of plasma proteins (CLP) and erythrocytes (CLE)--were tested in 136 healthy donors and 82 patients with untreated breast tumours for their applicability to detecting disturbances in antioxidant homeostasis in humans. The total antiradical capacity of water-soluble substances (ACW) and its urate-independent proportion (ACU) were lower (P <0.05) and CLP higher (P <0.001) in smokers in comparison to non-smokers. A significant negative correlation was found between the content of ascorbate in plasma and the intensity of CLP: r = -0.39, P <0.001. A significant reduction in ACU and increased values of CLP and CLE were seen according to the stage of disease in breast cancer patients. On the basis of these observations and model experiments we suggest that hydrogen peroxide-initiated chemiluminescence can serve as a parameter of oxidative modification of blood components and, in combination with the antioxidant parameters, can be used to describe the antioxidant homeostasis in humans and possibly to have value as a predictor of disease states.
Popov
I
I
Research Institute of Antioxidant Therapy, Berlin, Germany. poplew@snafu.de
Völker
H
H
Lewin
G
G
D000328:Adult; D000975:Antioxidants / Q000378:metabolism*; D019540:Area Under Curve; D001798:Blood Proteins / Q000032:analysis / Q000378:metabolism*; D001943:Breast Neoplasms / Q000097:blood*; D004912:Erythrocytes / Q000378:metabolism*; D005260:Female; D005609:Free Radicals / Q000032:analysis*; D006706:Homeostasis; D006801:Humans; D008163:Luminescent Measurements; D008297:Male; D012016:Reference Values; D012907:Smoking / Q000097:blood
D000975:Antioxidants; D001798:Blood Proteins; D005609:Free Radicals