Interaction of nuclear proteins with repeat sequences in the 5' flanking region of mouse muscle creatine kinase gene during aging.
The 5' flanking region of the mouse muscle creatine kinase (MCK) gene contains two repeat sequences-a mononucleotide repeat, (A)(22) (-2694 to -2673), and a tetranucleotide repeat, (GTTT)(8) (-2962 to -2931). We show here that these repeats in the mouse MCK gene bind to specific nuclear protein factors. Some of the factors interacting with these sequences are tissue-specific and show age-related decrease in the binding activity. Nonspecific competitor and heterologous DNA probes failed to compete out the complexes showing that the interaction is specific to the repeat sequences. These proteins may have a role in the expression of the gene during aging.
Shanti
K
K
Molecular Biology Laboratory, Banaras Hindu University, Varanasi, 221005, India.
Kanungo
M S
MS
D020121:5' Untranslated Regions / Q000502:physiology*; D000375:Aging / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001667:Binding, Competitive / Q000187:drug effects; D003402:Creatine Kinase / Q000235:genetics*; D015342:DNA Probes / Q000378:metabolism / Q000494:pharmacology; D005786:Gene Expression Regulation; D046911:Macromolecular Substances; D008297:Male; D051379:Mice; D008806:Mice, Inbred AKR; D009687:Nuclear Proteins / Q000378:metabolism*; D009928:Organ Specificity; D011485:Protein Binding / Q000187:drug effects / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid / Q000502:physiology*
D020121:5' Untranslated Regions; D015342:DNA Probes; D046911:Macromolecular Substances; D009687:Nuclear Proteins; D003402:Creatine Kinase