title: Comparison of Tn5397 from Clostridium difficile, Tn916 from Enterococcus faecalis and the CW459tet(M) element from Clostridium perfringens shows that they have similar conjugation regions but different insertion and excision modules.
abstract: Comparative analysis of the conjugative transposons Tn5397 from Clostridium difficile and Tn916 from Enterococcus faecalis, and the CW459tet(M) element from Clostridium perfringens, has revealed that these tetracycline-resistance elements are closely related. All three elements contain the tet(M) resistance gene and have sequence similarity throughout their central region. However, they have very different integration/excision modules. Instead of the int and xis genes that are found in Tn916, Tn5397 has a large resolvase gene, tndX. The C. perfringens element encodes the putative Int459 protein, which is a member of the integrase family of site-specific recombinases but is not closely related to Int from Tn916. Based on these studies it is concluded that the clostridial elements have a modular genetic organization and were derived independently from distinct mobile genetic elements.
authors.affiliation: Department of Microbiology, Eastman Dental Institute for Oral Health Care Sciences, University College London, University of London, 256 Gray's Inn Road, London WC1X 8LD, UK1.
authors.forename: Adam P
authors.initials: AP
authors.lastname: Roberts
authors.affiliation: Bacterial Pathogenesis Research Group, Department of Microbiology, Monash University, Vic 3800, Australia2.
authors.forename: Priscilla A
authors.initials: PA
authors.lastname: Johanesen
authors.affiliation: Bacterial Pathogenesis Research Group, Department of Microbiology, Monash University, Vic 3800, Australia2.
authors.forename: Dena
authors.initials: D
authors.lastname: Lyras
authors.affiliation: Department of Microbiology, Eastman Dental Institute for Oral Health Care Sciences, University College London, University of London, 256 Gray's Inn Road, London WC1X 8LD, UK1.
authors.forename: Peter
authors.initials: P
authors.lastname: Mullany
authors.affiliation: Bacterial Pathogenesis Research Group, Department of Microbiology, Monash University, Vic 3800, Australia2.
authors.forename: Julian I
authors.initials: JI
authors.lastname: Rood
mesh_terms: D001483:Base Sequence; D016360:Clostridioides difficile / Q000235:genetics*; D003016:Clostridium perfringens / Q000235:genetics*; D003227:Conjugation, Genetic; D004254:DNA Nucleotidyltransferases / Q000235:genetics; D004251:DNA Transposable Elements / Q000235:genetics*; D013293:Enterococcus faecalis / Q000235:genetics*; D019426:Integrases / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D045522:Recombinases; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D019895:Transposases / Q000235:genetics; D045564:Transposon Resolvases; D014764:Viral Proteins
chemical_list: D004251:DNA Transposable Elements; D045522:Recombinases; D014764:Viral Proteins; D004254:DNA Nucleotidyltransferases; D019426:Integrases; D019895:Transposases; D045564:Transposon Resolvases; C063911:excisionase; C115006:integron integrase IntI1