CoIIIEDTA- reduction by Desulfovibrio vulgaris and propagation of reactions involving dissolved sulfide and polysulfides.
The migration of 60Co, dominantly via transport of Co-EDTA complexes, into surface water and groundwater is a recognized concern at many nuclear production and storage sites. Reduction of CoIIIEDTA- to CoIIEDTA2- should decrease the mobility of 60Co in natural environments by stimulating ligand displacement with Fe(III) or Al(III) or by precipitation of CoSx in sulfidic environments. In this study, we examine direct (enzymatic) and indirect (metabolite) reduction processes of CoIIIEDTA- by the sulfate-reducing bacterium Desulfovibrio vulgaris. D. vulgaris reduces CoIIIEDTA- to CoIIEDTA2-, but growth using it as a terminal electron acceptor was not demonstrated. Rather than acting as a competing electron acceptor and limiting cobalt reduction, introducing sulfate with D. vulgaris enhances the reduction of CoIIIEDTA- as a result of sulfide production. Sulfide reduces CoIIIEDTA- in a pathway involving polysulfide formation and leads to a CoS precipitate. Thus, both direct and indirect (i.e., through the production of sulfide) microbial reduction pathways of CoIIIEDTA- may help to retard its migration within soils and waters.
Blessing
T C
TC
Soil Science Division, University of Idaho, Moscow, Idaho 83844-2339, USA.
Wielinga
B W
BW
Morra
M J
MJ
Fendorf
S
S
D001673:Biodegradation, Environmental; D003035:Cobalt / Q000378:metabolism*; D016969:Desulfovibrio vulgaris / Q000378:metabolism*; D004492:Edetic Acid / Q000378:metabolism*; D004787:Environmental Pollution / Q000517:prevention & control; D010084:Oxidation-Reduction; D013431:Sulfates / Q000378:metabolism; D013440:Sulfides / Q000378:metabolism*
D013431:Sulfates; D013440:Sulfides; C028222:cobalt-ethylenediamine tetraacetic acid chelate; D003035:Cobalt; C032915:polysulfide; D004492:Edetic Acid