Fabrication of ultra-thin polypyrrole-glucose oxidase film from supporting electrolyte-free monomer solution for potentiometric biosensing of glucose.
A simple electropolymerisation process is described for the fabrication of an ultra-thin ( approximately 55 nm) polypyrrole (PPy)-glucose oxidase (GOD) film in a supporting electrolyte-free monomer solution for potentiometric biosensing of glucose. The optimum conditions for growing the ultra-thin film include 0.1 M pyrrole, 55-110 U/ml GOD, an applied current density of 0.05 mA/cm(2) and an electrical charge of 25 mC/cm(2). Long-term storage of the biosensor in acetate buffer improved the sensitivity of the biosensor by a factor of approximately two. The biosensor can also be used repeatedly for over 2 months with little or no loss in sensitivity. The interference effect of ascorbic acid was successfully reduced by inclusion of an outer PPy-Cl layer.
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Centre for Electrochemical Research and Analytical Technology, School of Civic Engineering and Environment, University of Western Sydney, Nepean, PO Box 10, NSW 2747, Kingswood, Australia.
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D015374:Biosensing Techniques / Q000379:methods*; D004573:Electrolytes; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase; D011108:Polymers; D011199:Potentiometry; D011758:Pyrroles; D012996:Solutions
D004573:Electrolytes; D011108:Polymers; D011758:Pyrroles; D012996:Solutions; C067635:polypyrrole; D005949:Glucose Oxidase; D005947:Glucose