[Extracellular protein of propionic acid bacteria inhibits induced mutation in strains of Salmonella typhimurium].
A culture of propionic acid bacteria grown in a glucose-containing minimal medium, as well as the culture liquid and logarithmic-phase cells obtained from this culture, were found to inhibit the base pair substitution mutations induced by 4-nitroquinoline N-oxide, N-methyl-N'-nitro-N-nitrosoguanidine, and sodium azide and the frameshift mutations induced by 9-aminoacridine. The antimutagenic activity of the culture liquid (CL) was presumably due to the presence of an extracellular thermolabile protein with a molecular mass of no more than 12 kDa based on the facts that this activity considerably decreased after the treatment of the CL with pronase, its heating at 92 degrees C, and its dialysis in a cellulose sack, which retains substances with molecular masses greater than 12 kDa. The residual antimutagenic activity of the dialyzed culture liquid was probably related to the interaction of the mutagen with thiols, rather than to the presence of organic acids (acetic or propionic). Thiols may also contribute to the antimutagenic activity of the P. shermanii cells.
Vorob'eva
L I
LI
Department of Microbiology, Faculty of Biology, Moscow State University, Vorob'evy gory, Moscow, 119899 Russia.
Khodzhaev
E Iu
EIu
Ponomareva
G M
GM
D015112:4-Nitroquinoline-1-oxide / Q000633:toxicity; D016587:Antimutagenic Agents; D001426:Bacterial Proteins / Q000502:physiology*; D009153:Mutagens / Q000633:toxicity; D009154:Mutation; D011424:Propionibacterium / Q000378:metabolism*; D015363:Quinolones / Q000633:toxicity; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000235:genetics
C120195:4-nitroquinolone-1-oxide; D016587:Antimutagenic Agents; D001426:Bacterial Proteins; D009153:Mutagens; D015363:Quinolones; D015112:4-Nitroquinoline-1-oxide