cDNA cloning and expression of swine IL-7 from neonatal intestinal epithelium.
Swine IL-7 (sIL-7) cDNA has been isolated from neonatal intestinal epithelium by reverse transcription polymerase chain reaction (RT-PCR) with designated primers based on the conserved sequences of the other mammalian IL-7. Recombinant sIL-7 was able to induce the bone marrow cell proliferation like human IL-7. The complete open reading frame contains 531 bp coding deduced 176 amino acid residues, with a calculated molecular mass of 20.1 kDa. The observed similarity between swine and human IL-7 sequences (66% identical and 89% conserved) suggests that a study on the immune responses correlated with IL-7 in the gut of swine will be valuable to understand their mechanisms in human. By RT-PCR using the constructed specific primers to sIL-7, sIL-7 mRNA was confirmed to be expressed in swine intestine, thymus, kidney and skin but not in the heart.
Ueha
S
S
Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohuku University, Sendai, Japan.
Kitazawa
H
H
Tomioka
Y
Y
Kawai
Y
Y
Saito
T
T
Itoh
T
T
D000595:Amino Acid Sequence; D000818:Animals; D000831:Animals, Newborn; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics* / Q000302:isolation & purification; D004848:Epithelium / Q000276:immunology; D015870:Gene Expression; D006801:Humans; D015851:Interleukin-7 / Q000737:chemistry / Q000235:genetics* / Q000494:pharmacology; D007422:Intestines / Q000276:immunology*; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016366:Open Reading Frames; D011994:Recombinant Proteins / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D012756:Sheep; D013045:Species Specificity; D013552:Swine
D017931:DNA Primers; D018076:DNA, Complementary; D015851:Interleukin-7; D011994:Recombinant Proteins