title: 3-Methyl-5-hydroxy-5-trichloromethyl-1H-1-pyrazolcarboxyamide induces antinociception.
abstract: The antinociceptive action of a novel pyrazole-derived compound, 3-methyl-5-hydroxy-5-trichloromethyl-1H-1-pyrazolcarboxyamide (MPCA) was evaluated using the formalin and tail-immersion tests in mice. Anti-inflammatory activity was assessed by paw plethysmometry in adult rats using the carrageenin-induced paw edema test. Subcutaneous administration of MPCA (22, 66, and 200 mg/kg) induced a dose-dependent decrease in the time spent licking during the neurogenic and inflammatory phases of the formalin test, and preadministration of naloxone (1 mg/kg, sc) did not prevent MPCA-induced (200 mg/kg, sc) antinociception. Naloxone decreased the spontaneous locomotor activity of mice, while MPCA had no effect on locomotion. In contrast, administration of the opioid antagonist caused a significant increase in the locomotor behavior of mice previously injected with MPCA. MPCA was devoid of antinociceptive action by the tail-immersion test and of anti-inflammatory activity. Moreover, MPCA had no effect on the motor performance of mice in the rotarod test. These results suggest that MPCA induces antinociception in the neurogenic and inflammatory phases of the formalin test, an effect that does not involve opioid receptors.
authors.affiliation: Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, 97105-900 RS, Santa Maria, Brazil.
authors.forename: F R
authors.initials: FR
authors.lastname: de Souza
authors.forename: M R
authors.initials: MR
authors.lastname: Fighera
authors.forename: T T
authors.initials: TT
authors.lastname: Lima
authors.forename: J
authors.initials: J
authors.lastname: de Bastiani
authors.forename: I B
authors.initials: IB
authors.lastname: Barcellos
authors.forename: C E
authors.initials: CE
authors.lastname: Almeida
authors.forename: M R
authors.initials: MR
authors.lastname: de Oliveira
authors.forename: H G
authors.initials: HG
authors.lastname: Bonacorso
authors.forename: A E
authors.initials: AE
authors.lastname: Flores
authors.forename: C F
authors.initials: CF
authors.lastname: de Mello
mesh_terms: D000700:Analgesics / Q000494:pharmacology*; D000701:Analgesics, Opioid / Q000494:pharmacology; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D002351:Carrageenan; D004487:Edema / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control; D005557:Formaldehyde; D007101:Immersion; D008297:Male; D051379:Mice; D009020:Morphine / Q000494:pharmacology; D009270:Naloxone / Q000494:pharmacology; D009292:Narcotic Antagonists / Q000494:pharmacology; D010147:Pain Measurement / Q000187:drug effects; D004856:Postural Balance / Q000187:drug effects; D011720:Pyrazoles / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: C430590:3-methyl-5-hydroxy-5-trichloromethyl-1H-1-pyrazolcarboxyamide; D000700:Analgesics; D000701:Analgesics, Opioid; D000894:Anti-Inflammatory Agents, Non-Steroidal; D009292:Narcotic Antagonists; D011720:Pyrazoles; D005557:Formaldehyde; D009270:Naloxone; D009020:Morphine; D002351:Carrageenan