Rapid, high-throughput extraction of bacterial genomic DNA from selective-enrichment culture media.
OBJECTIVE
To create a fast, sensitive and inexpensive high-throughput method for the extraction of bacterial genomic DNA from selective-enrichment culture media.


RESULTS
Lysis of bacteria was achieved using guanidinium isothiocyanate, and DNA was extracted using 96-well glass microfibre filtration plates. Extraction-PCR detected the presence of 1 cfu Yersinia ruckeri and 16 cfu Lactococcus garvieae 200 microl(-1) sample of selective-enrichment medium.


CONCLUSIONS
An efficient method for high-throughput extraction of bacterial genomic DNA from selective-enrichment culture media was achieved.


CONCLUSIONS
This method enables detection of covert bacterial infections in fish. The simultaneous extraction of large numbers of samples allows for its use in bacterial monitoring programmes and quarantine.
Wilson
T
T
Fish Health Unit, Tasmanian Aquaculture and Fisheries Institute, University of Tasmania, Launceston, Australia. Teresa.Wilson@dpiwe.tas.gov.au
Carson
J
J
D001419:Bacteria / Q000254:growth & development / Q000302:isolation & purification*; D003470:Culture Media; D004269:DNA, Bacterial / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity
D003470:Culture Media; D004269:DNA, Bacterial