title: Backbone dynamics of the channel-forming antibiotic zervamicin IIB studied by 15N NMR relaxation.
abstract: The backbone dynamics of the channel-forming peptide antibiotic zervamicin IIB (Zrv-IIB) in methanol were studied by 15N nuclear magnetic resonance relaxation measurements at 11.7, 14.1 and 18.8 T magnetic fields. The anisotropic overall rotation of the peptide was characterized based on 15N relaxation data and by hydrodynamic calculations. 'Model-free' analysis of the relaxation data showed that the peptide is fairly rigid on a sub-nanosecond time-scale. The residues from the polar side of Zrv-IIB helix are involved in micro-millisecond time-scale conformational exchange. The conformational exchange observed might indicate intramolecular processes or specific intermolecular interactions of potential relevance to Zrv-IIB ion channel formation.
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, 117997 Moscow, Russia.
authors.forename: D M
authors.initials: DM
authors.lastname: Korzhnev
authors.forename: E V
authors.initials: EV
authors.lastname: Bocharov
authors.forename: A V
authors.initials: AV
authors.lastname: Zhuravlyova
authors.forename: V Y
authors.initials: VY
authors.lastname: Orekhov
authors.forename: T V
authors.initials: TV
authors.lastname: Ovchinnikova
authors.forename: M
authors.initials: M
authors.lastname: Billeter
authors.forename: A S
authors.initials: AS
authors.lastname: Arseniev
mesh_terms: D016880:Anisotropy; D000900:Anti-Bacterial Agents / Q000737:chemistry*; D003198:Computer Simulation; D007473:Ion Channels / Q000737:chemistry*; D000432:Methanol / Q000737:chemistry; D008968:Molecular Conformation; D009587:Nitrogen Isotopes; D019906:Nuclear Magnetic Resonance, Biomolecular; D054848:Peptaibols; D010455:Peptides; D012399:Rotation
chemical_list: D000900:Anti-Bacterial Agents; D007473:Ion Channels; D009587:Nitrogen Isotopes; D054848:Peptaibols; D010455:Peptides; C077077:zervamicin IIB; D000432:Methanol