title: Assessment of toxicity and mutagenicity in air particulate matter from an urban industrial area in the coast of the Rio de la Plata.
abstract: Chemical characterization and effects assessment of semivolatile organic compounds in organic extracts from air particulate matter from the region of the greater La Plata area was undertaken. Effects covered the study of mutagenicity with the Ames test (Salmonella typhimurium TA100 and TA98 strains with metabolic activation by S9) and cytotoxicity using the Tetrahymena pyriformis test system (growth rate, cell volume, and cell respiration). Chemical analysis of organic extracts was done using gas chromatography. Results demonstrate the presence of polycyclic aromatic hydrocarbons (PAH) in the matrix, high mutagenicity, and cytotoxicity. A higher mutagenic activity detected with TA98 and TA100 strains is associated with an increment of total PAH and total five or six ring PAH content, respectively. Linked with it, a PAH dependent toxicity on Tetrahymena pyriformis has been observed. This cell system proved to be very sensitive. From the results obtained with the cell respiration assay with T. pyriformis it appears that uncoupling agents are present in the samples. The results of this study indicate that air particulate matter from the Rio de La Plata area contains significant genotoxic and cytotoxic activity probably due to the presence of PAHs.
authors.affiliation: Department of Human Exposure Research and Epidemiology, UFZ Centre for Environmental Research Leipzig, Germany. amueller@expo.ufz.de
authors.forename: A
authors.initials: A
authors.lastname: Müller
authors.forename: P
authors.initials: P
authors.lastname: Alzuet
authors.forename: O
authors.initials: O
authors.lastname: Herbarth
authors.forename: A
authors.initials: A
authors.lastname: Ronco
mesh_terms: D000393:Air Pollutants / Q000737:chemistry / Q000633:toxicity*; D000818:Animals; D001118:Argentina; D004784:Environmental Monitoring; D006801:Humans; D007221:Industry; D009152:Mutagenicity Tests; D011084:Polycyclic Aromatic Hydrocarbons / Q000737:chemistry / Q000633:toxicity*; D012475:Salmonella / Q000187:drug effects*; D013769:Tetrahymena pyriformis / Q000187:drug effects*; D014504:Urban Health
chemical_list: D000393:Air Pollutants; D011084:Polycyclic Aromatic Hydrocarbons