Stimulation of contraction of pregnant rat uterus in vitro by non-dechlorinated and microbially dechlorinated mixtures of polychlorinated biphenyls.
A previous study of six polychlorinated biphenyl (PCB) congeners showed that PCBs with four or fewer chlorines and ortho substitution stimulate uterine contraction frequency in vitro, whereas congeners with a greater number of chlorines or non-ortho substitution are inactive in vitro. We tested the hypothesis that PCB mixtures stimulate uterine contractions in a manner inversely related to the degree of chlorination and the presence of chlorines in the ortho- position of the biphenyl constituents of the mixtures. Uterine strips from pregnant rats were suspended in standard muscle baths and analyzed for changes in isometric contractions in response to in vitro exposure to commercial PCB mixtures (Aroclors) and their dechlorinated products after microbial degradation. The PCB mixtures Aroclor 1242, 1248, and 1254 significantly stimulated uterine contraction frequency, and the least chlorinated mixture, Aroclor 1242, was the most potent stimulant. Microbes from Hudson River sediment dechlorinated Aroclor 1242 and Aroclor 1254 under reducing conditions to produce mixtures with an increased proportion of ortho-substituted congeners with one or two chlorine substitutions. The PCB mixtures that had undergone microbial reductive dechlorination stimulated uterine contraction frequency to a significantly greater extent than the parent mixtures. These results show that increased uterotonic activity was associated with decreased chlorination and increased ortho substitution of the biphenyl constituents of the mixtures.
Bae
J
J
Toxicology Program, Department of Environmental Health Sciences, The University of Michigan, Ann Arbor, MI 48109-2029, USA.
Mousa
M A
MA
Quensen
J F
JF
Boyd
S A
SA
Loch-Caruso
R
R
D000818:Animals; D001140:Aroclors / Q000737:chemistry / Q000494:pharmacology; D004785:Environmental Pollutants / Q000494:pharmacology*; D005260:Female; D009119:Muscle Contraction / Q000187:drug effects*; D011078:Polychlorinated Biphenyls / Q000737:chemistry / Q000494:pharmacology*; D011247:Pregnancy; D051381:Rats; D013329:Structure-Activity Relationship; D014599:Uterus / Q000187:drug effects*
D001140:Aroclors; D004785:Environmental Pollutants; D011078:Polychlorinated Biphenyls