Macroencapsulation of rat islets without alteration of insulin secretion kinetics.
Transplantation of encapsulated islets may restore endogenous insulin secretion in type 1 diabetics with no need of lifetime immunosuppression of the recipient. A biomaterial should be developed which combined immunoisolation with rapid and efficient diffusion of glucose and insulin. Rat islets were macroencapsulated in capillaries (molecular cut off 50 kD) of differently modified polysulphone. Macroencapsulated islets were perifused to study the kinetics of glucose induced insulin secretion into the perifusion medium. Blending polysulphone (PSU) with poly vinyl pyrrolidone or sodium dodecyl sulphate was not suited for islet macroencapsulation since glucose induced insulin release was absent after encapsulation. Hydroxy methylation (CH2OH) of PSU improved the secretory behaviour of macroencapsulated islets depending on the degree of substitution (DS). At 0.8 DS glucose induced insulin secretion was delayed and inefficient. At maximal degrees of PSU-substitution (1.8) the kinetics of insulin release and the efficiency of insulin release were very similar to that observed of free floating islets. In conclusion, highly substituted hydroxy methylated polysulphone allows a rapid and efficient insulin release after macroencapsulation and is suited for the further development of a bioartificial pancreas.
Lembert
N
N
Department of Pharmacology, University of Tübingen, Germany. nicolas.lembert@uni-tuebingen.de
Wesche
J
J
Petersen
P
P
Zschocke
P
P
Enderle
A
A
Planck
H
H
Ammon
H P
HP
D000818:Animals; D002214:Capsules; D004058:Diffusion; D005947:Glucose / Q000494:pharmacology; D006652:Histological Techniques; D006900:Hydroxylation; D066298:In Vitro Techniques; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D008745:Methylation; D008863:Microspheres; D011108:Polymers / Q000378:metabolism; D051381:Rats; D012016:Reference Values; D013450:Sulfones / Q000378:metabolism
D002214:Capsules; D007328:Insulin; D011108:Polymers; D013450:Sulfones; C017662:polysulfone P 1700; D005947:Glucose