[Contribution of the laboratory to the epidemiologic study of bacterial infections].
The laboratory plays a significant role in the epidemiologic investigations by the comparative analysis of the bacterial strains involved in the outbreaks. Recently, the use of molecular analysis methods provided better performance than traditional phenotypic methods which are still used as preliminary tests because of their relatively low cost and technical simplicity. These analyses deal with either the whole chromosome of the bacterium, plasmids or particular genes. The classification of these methods runs up against the lack of consensus concerning their nomenclature. A clearer denomination based upon the technique responsible for revealing the polymorphisms of these various targets, makes it possible to divide these methods in two principal groups: methods of RFLP (based on the fragments resulting from digestion with restriction enzymes) and methods of AFLP (based on the products of amplification by PCR). The knowledge of the typability of the strains and the qualities of these various methods, particularly their discriminatory power, is essential to the accuracy of the laboratory analysis in the investigations of outbreaks.
Biron
M
M
Laboratoire de Microbiologie, Hôpital Necker-Enfants Malades, 149 rue de Sèvres, 75015 Paris, France.
Berche
P
P
Ferroni
A
A
D001419:Bacteria / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D001424:Bacterial Infections / Q000453:epidemiology* / Q000382:microbiology; D001426:Bacterial Proteins / Q000032:analysis; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000032:analysis; D004196:Disease Outbreaks; D004352:Drug Resistance, Microbial; D004586:Electrophoresis; D004798:Enzymes / Q000032:analysis; D006801:Humans; D010957:Plasmids / Q000235:genetics / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D009626:Terminology as Topic
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004798:Enzymes