title: Quantifying DNA damage by gel electrophoresis, electronic imaging and number-average length analysis.
abstract: DNA damages that can be converted to single- or double strand breaks can be quantified by separating DNA by gel electrophoresis and obtaining a quantitative image of the resulting distribution of DNA in the gel. We review the theory of this method and discuss its implementation, including the charge-coupled device (CCD) camera systems we developed to acquire images of fluorophore labeled DNA.
authors.affiliation: Biology Department, Brookhaven National Laboratory, Upton, NY 11973, USA. jcs@bnl.gov
authors.forename: J C
authors.initials: JC
authors.lastname: Sutherland
authors.forename: D C
authors.initials: DC
authors.lastname: Monteleone
authors.forename: J G
authors.initials: JG
authors.lastname: Trunk
authors.forename: P V
authors.initials: PV
authors.lastname: Bennett
authors.forename: B M
authors.initials: BM
authors.lastname: Sutherland
mesh_terms: D004247:DNA / Q000032:analysis / Q000302:isolation & purification; D004249:DNA Damage; D004587:Electrophoresis, Agar Gel / Q000295:instrumentation; D019075:Electrophoresis, Capillary; D005456:Fluorescent Dyes; D007091:Image Processing, Computer-Assisted / Q000295:instrumentation; D007834:Lasers; D010781:Photography; D012680:Sensitivity and Specificity
chemical_list: D005456:Fluorescent Dyes; D004247:DNA