title: Canine pubic symphysiodesis: investigation of electrocautery dose response by histologic examination and temperature measurement.
abstract: OBJECTIVE
  To determine thermal necrosis zone and the maximal temperature achieved with various electrocautery doses applied to the pubic symphysis of immature dogs, and to establish the minimal electrocautery dose needed to affect thermal necrosis of the germinal chondrocytes of the immature canine pubis.
  
  
  METHODS
  A randomized, controlled, prospective study.
  
  
  METHODS
  Twelve puppies, 15 to 17 weeks of age.
  
  
  METHODS
  Each treated puppy was randomly selected to receive 3 of 33 possible doses of electrical current applied at uniform intervals along the pelvic symphysis. Three treatment sites were sham-operated controls. All treatment zones were biopsied 7 days after application of electrocautery. Confocal laser microscopy with calcein green and ethidium homodimer red stains were used to determine cell type and viability within treatment and control zones. The physeal diameter and thermal necrosis zone were measured. Thermal necrosis zone and dose, and temperature and dose were compared using regression analysis. Prediction equation analysis was used to establish dose recommendations.
  
  
  RESULTS
  Doses of 40 W produced a highly significant linear relationship (R(2) =.88, P <.01) with thermal necrosis zone, and a significant linear relationship (R(2) =.76, P <.02) with maximal temperature.
  
  
  CONCLUSIONS
  The thermal necrosis zone within the pelvic symphysis and maximal temperature achieved can be reliably predicted using electrocautery doses of 40 W. For application of electrocautery to induce pubic symphysiodesis, doses should be chosen based on measured or estimated physeal size. Using prediction equation analysis, the surgeon can plan mean thermal necrosis zone with acceptable variance.
  
  
  CONCLUSIONS
  Unipolar needle electrocautery can be used to reliably achieve symphyseal necrosis. Prediction equation analysis can be used to determine 40-W doses.
authors.affiliation: Department of Surgical Sciences, University of Wisconsin, School of Veterinary Medicine, 2015 Linden Drive West, Madison, WI 53706, USA.
authors.forename: A J
authors.initials: AJ
authors.lastname: Patricelli
authors.forename: R T
authors.initials: RT
authors.lastname: Dueland
authors.forename: Y
authors.initials: Y
authors.lastname: Lu
authors.forename: J
authors.initials: J
authors.lastname: Fialkowski
authors.forename: K G
authors.initials: KG
authors.lastname: Mathews
mesh_terms: D000818:Animals; D001174:Arthrodesis / Q000379:methods / Q000592:standards / Q000662:veterinary*; D004283:Dog Diseases / Q000601:surgery*; D004285:Dogs; D004564:Electrocoagulation / Q000379:methods / Q000662:veterinary*; D018613:Microscopy, Confocal; D009336:Necrosis; D011446:Prospective Studies; D011631:Pubic Symphysis / Q000473:pathology / Q000601:surgery*; D013194:Staining and Labeling; D013696:Temperature