[Effects of receptor-mediated endocytosis on macrophage membrane potential, cytosolic pH and lysosomal pH].
The effects of Con A, WGA, Zymosan A on membrane potential, cytosolic pH and lysosomal pH of cultured mouse macrophages were measured with fluorescence method. The results showed all the three kinds of ligands caused macrophage membrane potential hyperpolarization, cytosolic acidification and lysosomal alkalization, but reached their stabilized conditions at different times. The acidification of cytosl might inhibit further endocytosis. Alkalization of lysosomes was a basic factor for exocytosis of lysosomal contents. These receptor-mediated induced changes reflected cellular self-regulation and self-protection.
Lei
G H
GH
Central Laboratory, First Military Medical University, Guangzhou 510515.
Piao
Y J
YJ
Wu
J C
JC
Bao
Y Y
YY
Huang
H
H
Zhang
W
W
D000818:Animals; D002478:Cells, Cultured; D003208:Concanavalin A; D003593:Cytoplasm / Q000378:metabolism; D004705:Endocytosis / Q000502:physiology*; D006863:Hydrogen-Ion Concentration; D008247:Lysosomes / Q000378:metabolism*; D008264:Macrophages / Q000166:cytology / Q000502:physiology*; D008564:Membrane Potentials; D051379:Mice; D011956:Receptors, Cell Surface / Q000502:physiology*
D011956:Receptors, Cell Surface; D003208:Concanavalin A