title: Clearance of increased serum advanced glycosylation end products in patients with end stage renal disease by hemodialysis.
abstract: OBJECTIVE
  To study the effect of removal of hemodialysis using acetate membrane on serum advanced glycosylation end products (AGEP) in 36 patients (59.1 +/- 1.6) with end stage renal disease (ESRD).
  
  
  METHODS
  Serum AGEP levels were determined with quantitative fluorescence spectrometry, flow injection analysis (FIA), and competitive enzyme-linked immunosorbent assay (ELISA) using a polyclonal antibody directed against AGEP.
  
  
  RESULTS
  The serum AGEP levels in patients with ESRD quantified by fluorescence spectrometry, FIA, and ELISA were higher than those in controls [(25 +/- 5) vs (7.5 +/- 1.5), (6.1 +/- 1.8) vs (1.4 +/- 0.5), and (37 +/- 20) vs (9 +/- 10) kU/L, respectively] and markedly reduced to [(22 +/- 6), (4.2 +/- 1.4), and (19 +/- 14) kU/L, respectively] after hemodialysis.
  
  
  CONCLUSIONS
  Increased serum AGEP levels in the circulation of patients with ESRD were reduced effectively by hemodialysis.
authors.affiliation: Department of Cardiology, First Affiliated Hospital, Nanjing Railway Medical College, Nanjing 210009, China. nfliu@public1.ptt.js.cn
authors.forename: N F
authors.initials: NF
authors.lastname: Liu
authors.forename: Z L
authors.initials: ZL
authors.lastname: Sun
authors.forename: B C
authors.initials: BC
authors.lastname: Liu
mesh_terms: D000368:Aged; D002908:Chronic Disease; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D017022:Flow Injection Analysis; D005921:Glomerulonephritis / Q000150:complications; D017127:Glycation End Products, Advanced / Q000097:blood*; D006801:Humans; D007676:Kidney Failure, Chronic / Q000097:blood* / Q000209:etiology / Q000628:therapy; D008297:Male; D008875:Middle Aged; D009395:Nephritis, Interstitial / Q000150:complications; D006435:Renal Dialysis; D013050:Spectrometry, Fluorescence
chemical_list: D017127:Glycation End Products, Advanced