Identification of an intragenic integration site for foreign gene expression in recombinant Streptococcus gordonii strains.
A new intragenic chromosomal integration site within the lacG gene of the lac operon has been identified in Streptococcus gordonii for use in the expression of foreign genes. Introduction of a portion of the Streptococcus pyogenes emm6 gene into the lacG locus resulted in the lactose-inducible surface expression of the S. pyogenes M6 protein. This result demonstrates the ability to modulate the in vitro or in vivo expression of a foreign gene in a S. gordonii recombinant using a biosynthetic metabolite.
Bolken
T C
TC
SIGA Research Laboratories, Corvallis, Oregon 97333, USA.
Franke
C A
CA
Zeller
G O
GO
Hruby
D E
DE
D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins / Q000096:biosynthesis / Q000235:genetics*; D002352:Carrier Proteins / Q000096:biosynthesis / Q000235:genetics*; D015870:Gene Expression; D020868:Gene Silencing; D005798:Genes, Bacterial; D006026:Glycoside Hydrolases; D007763:Lac Operon; D016254:Mutagenesis, Insertional; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000096:biosynthesis; D013291:Streptococcus / Q000235:genetics*; D013297:Streptococcus pyogenes / Q000235:genetics; D014169:Transformation, Bacterial; D001616:beta-Galactosidase / Q000235:genetics*
D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D002352:Carrier Proteins; D011994:Recombinant Proteins; C014689:streptococcal M protein; D006026:Glycoside Hydrolases; D001616:beta-Galactosidase; C022361:6-phospho-beta-galactosidase