Characterization of a Na+-Ca2+ exchanger NCX1 isoform in bovine fasciculata cells of adrenal gland.
The Na+-Ca2+ exchanger (NCX) is well known to regulate intracellular Ca2+ concentration in many excitable cells. To date, three members of the NCX exchanger gene family have been identified: NCX1, NCX2 and NCX3. In zona fasciculata of the bovine adrenal gland, a Na+-Ca2+ exchanger has been recently found to display biochemical similarities with the NCX1 exchanger of renal basolateral cells. Although a Na+-Ca2+ exchanger has already been characterized in zona medulla of adrenal gland, such an exchanger has never been studied in the adrenal cortex. Thus, we have used a reverse transcriptase-polymerase chain reaction (RT-PCR) assay with specific NCX primers on fasciculata cell cultures. The sequencing of the amplified fragment revealed, for the first time, the presence of a NACA3 isoform of the NCX1 exchanger in the adrenal cortex, similar to the one found in adrenal chromaffin cells and in renal cortex. This isoform is 92 and 94% identical in the portion compared with kidney Na+-Ca2+ exchanger NACA3 of rat and pig, respectively.
Morales
A
A
Laboratoire de Physiologie des Régulations Energétiques, Cellulaires et Moléculaires, UMR 5578 CNRS-UCB LYON 1, Faculté des Sciences, Villeurbanne, France.
Lachuer
J
J
Bilbaut
A
A
Georges
B
B
Andrieu
J L
JL
Diez
J
J
Ojeda
C
C
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002478:Cells, Cultured; D005091:Exons; D008969:Molecular Sequence Data; D009206:Myocardium / Q000378:metabolism; D020033:Protein Isoforms / Q000235:genetics; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D019831:Sodium-Calcium Exchanger / Q000235:genetics / Q000378:metabolism*; D013552:Swine; D015383:Zona Fasciculata / Q000378:metabolism*
D020033:Protein Isoforms; D019831:Sodium-Calcium Exchanger; C110374:sodium-calcium exchanger 1