title: Structure of epiglucan, a highly side-chain/branched (1 --> 3;1 --> 6)-beta-glucan from the micro fungus Epicoccum nigrum Ehrenb. ex Schlecht.
abstract: The extracellular fungal polysaccharide, epiglucan, synthesised by Epicoccum nigrum is a side-chain/branched (1 --> 3;1 --> 6)-D-beta-glucan. Methylation analysis, 13C DEPT NMR and specific enzymic digestion data show slight variation in branching frequency among the epiglucans from the three strains examined. The (1 --> 3)-beta-linked backbone has (1 --> 6)-beta-linked branches at frequencies greater than the homologous glucans, scleroglucan and schizophyllan, from Sclerotium spp. and Schizophyllum commune, respectively. The structural analyses do not allow a distinction to be made between structures I and II. [structures: see text] Epiglucan displays non-Newtonian shear thinning rheological properties, typical of these glucans.
authors.affiliation: Biotechnology Research Centre, La Trobe University, Bendigo, Vic., Australia.
authors.forename: F
authors.initials: F
authors.lastname: Schmid
authors.forename: B A
authors.initials: BA
authors.lastname: Stone
authors.forename: B M
authors.initials: BM
authors.lastname: McDougall
authors.forename: A
authors.initials: A
authors.lastname: Bacic
authors.forename: K L
authors.initials: KL
authors.lastname: Martin
authors.forename: R T
authors.initials: RT
authors.lastname: Brownlee
authors.forename: E
authors.initials: E
authors.lastname: Chai
authors.forename: R J
authors.initials: RJ
authors.lastname: Seviour
mesh_terms: D001203:Ascomycota / Q000737:chemistry*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002855:Chromatography, Thin Layer; D043326:Glucan 1,3-beta-Glucosidase; D005936:Glucans / Q000737:chemistry* / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D015394:Molecular Structure; D013055:Spectrophotometry, Infrared; D001617:beta-Glucosidase / Q000378:metabolism
chemical_list: D005936:Glucans; C033671:epiglucan; D001617:beta-Glucosidase; D043326:Glucan 1,3-beta-Glucosidase