[Determination of 10-hydroxy-2-decenoic acid (10-HDA) in Chinese traditional medicine preparations by high performance liquid chromatography].
This paper reports the quantitative determination of 10-HDA in Chinese traditional medicine preparations by HPLC. Spherisorb C18 column was used. The mobile phase consisted of methanol-distilled water and phosphoric acid(45:55:0.5, V/V). The elute was monitored at 210 nm. The good linearity was shown between the concentration of 10-HDA and peak area in the concentration range of 6.0-30.0 mg/L (r = 0.9999, n = 5). The detection limit was 0.2 mg/L (S:N = 3:1). The within-day and between-day RSD were 2.1%-2.2% and 2.9%-4.0%, respectively. Peaks of 10-HDA and impurities in these samples were separated completely. This method is simple, sensitive and accurate.
Yang
J
J
Hangzhou Institute for Drug Control, Hangzhou, 310014.
Chen
L
L
D000970:Antineoplastic Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005229:Fatty Acids, Monounsaturated / Q000032:analysis*
D000970:Antineoplastic Agents; D004365:Drugs, Chinese Herbal; D005229:Fatty Acids, Monounsaturated; C055543:10-hydroxy-2-decenoic acid