title: Naturally-occurring Neospora caninum infection in an adult sheep and her twin fetuses.
abstract: Neospora caninum tissue cysts were found in the brains of surgically delivered twin fetuses at 119 days of gestation. In the brains of both fetuses, there was an inflammatory reaction involving perivascular cuffings of mononuclear cells, glial nodules. The dam of these fetuses died because of metritis. Histopathological examination of the ewe revealed N. caninum tissue cysts and focal gliosis with mononuclear cell cuffings. A N. caninum-specific DNA fragment was detected in a brain homogenate of the ewe by the polymerase chain reaction method. This is the first report of N. caninum infection in twin ovine fetuses and in an adult sheep.
authors.affiliation: Department of Veterinary Pathology, Obihiro University of Agriculture and Veterinary Medicine, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Kobayashi
authors.forename: M
authors.initials: M
authors.lastname: Yamada
authors.forename: Y
authors.initials: Y
authors.lastname: Omata
authors.forename: T
authors.initials: T
authors.lastname: Koyama
authors.forename: A
authors.initials: A
authors.lastname: Saito
authors.forename: T
authors.initials: T
authors.lastname: Matsuda
authors.forename: K
authors.initials: K
authors.lastname: Okuyama
authors.forename: S
authors.initials: S
authors.lastname: Fujimoto
authors.forename: H
authors.initials: H
authors.lastname: Furuoka
authors.forename: T
authors.initials: T
authors.lastname: Matsui
mesh_terms: D000818:Animals; D001921:Brain / Q000469:parasitology; D003048:Coccidiosis / Q000469:parasitology / Q000635:transmission / Q000662:veterinary*; D016054:DNA, Protozoan / Q000032:analysis / Q000302:isolation & purification; D017809:Fatal Outcome; D005260:Female; D005315:Fetal Diseases / Q000469:parasitology / Q000662:veterinary*; D005911:Gliosis / Q000469:parasitology / Q000662:veterinary; D018445:Infectious Disease Transmission, Vertical / Q000662:veterinary*; D018688:Neospora / Q000235:genetics / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011247:Pregnancy; D015597:Pregnancy Complications, Parasitic / Q000469:parasitology / Q000662:veterinary*; D012756:Sheep; D012757:Sheep Diseases / Q000469:parasitology* / Q000635:transmission
chemical_list: D016054:DNA, Protozoan