Dethiosulfovibrio russensis sp. nov., Dethosulfovibrio marinus sp. nov. and Dethosulfovibrio acidaminovorans sp. nov., novel anaerobic, thiosulfate- and sulfur-reducing bacteria isolated from 'Thiodendron' sulfur mats in different saline environments.
Four strains of strictly anaerobic, sulfur- and thiosulfate-reducing bacteria, SR12T, SR13, SR15T and WS100T, were isolated from 'Thiodendron' sulfur mats obtained from different saline environments. All isolates were motile, Gram-negative, non-spore-forming curved rods with pointed or rounded ends. The sizes of cells varied from 0.9 x 3-5 microm for strains SR12T, SR13 and SR15T to 0.9 x 4.8 microm for strain WS100T. All strains could form long spiral filamentous cells up to 70-110 microm during the early stage of growth. All strains were motile by a tumbling movement and possessed lateral flagella arranged at the concave side of cells. Incomplete cross-septa were distinctive features of all strains. Growth occurred at temperatures of 10-40 degrees C with an optimum at 28 degrees C. The pH limits for growth were 5.5 to 8.0, with optimal growth at pH 6.5-7.0. All isolates were obligately anaerobic and slightly halophilic and grew in media containing 0.5-5% NaCl with an optimum at 2% NaCl. All strains were chemoorganoheterotrophic, having a fermentative type of metabolism and utilized proteins, peptides, amino acids and some organic acids, but not sugars, fatty acids or alcohols. Some organic substrates (isoleucine, valine, alanine, glutamate) were utilized only by strain SR12T in the presence of sulfur or thiosulfate. Fermentation of citrate yielded mainly acetate, CO2 and H2. Sulfur and thiosulfate were reduced to hydrogen sulfide during the fermentation of organic substances, which increased cell yields and growth rates. Sulfate, sulfite, fumarate, nitrate, Fe2O3, MnO2, DMSO and elemental selenium were not used as electron acceptors by these strains. The G+C contents of the DNA were 51 mol% for strains SR12T, SR13 and SR15T and 52 mol% for strain WS100T. Based on morphological, physiological and phylogenetic similarities, all four isolates could be assigned to three new species of the genus Dethiosulfovibrio, named Dethiosulfovibrio russensis (type strain DSM 12538T), Dethiosulfovibrio marinus (type strain DSM 12537T) and Dethiosulfovibrio acidaminovorans (type strain DSM 12590T).
Surkov
A V
AV
Laboratory of Ecology and Geochemical Activity of Microorganisms, Institute of Microbiology of Russian Academy of Sciences, Moscow, Russia.
Dubinina
G A
GA
Lysenko
A M
AM
Glöckner
F O
FO
Kuever
J
J
D015373:Bacterial Typing Techniques; D001482:Base Composition; D006090:Gram-Negative Bacteria / Q000145:classification* / Q000302:isolation & purification / Q000378:metabolism / Q000648:ultrastructure; D008900:Mineral Waters / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010084:Oxidation-Reduction; D017422:Sequence Analysis, DNA; D013455:Sulfur / Q000378:metabolism*; D009626:Terminology as Topic; D013885:Thiosulfates / Q000378:metabolism*; D014871:Water Microbiology
D008900:Mineral Waters; D013885:Thiosulfates; D013455:Sulfur