Capacitive detection of glucose using molecularly imprinted polymers.
A novel glucose biosensor based on capacitive detection has been developed using molecularly imprinted polymers. The sensitive layer was prepared by electropolymerization of o-phenylenediamine on a gold electrode in the presence of the template (glucose). Cyclic voltammetry and capacitive measurements monitored the process of electropolymerization. Surface uncovered areas were plugged with 1-dodecanethiol to make the layer dense, and the insulating properties of the layer were studied in the presence of redox couples. The template molecules and the nonbound thiol were removed from the modified electrode surface by washing with distilled water. A capacitance decrease could be obtained after injection of glucose. The electrode constructed similarly but with ascorbic acid or fructose only showed a small response compared with glucose. The stability and reproducibility of the biosensor were also investigated.
Cheng
Z
Z
Laboratory of Electroanalytical Chemistry and National Analytical Research Center of Electrochemistry and Spectroscopy, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, Jilin, People's Republic of China.
Wang
E
E
Yang
X
X
D015374:Biosensing Techniques / Q000379:methods*; D004553:Electric Conductivity; D004563:Electrochemistry; D004579:Electron Transport; D005947:Glucose / Q000032:analysis*; D006046:Gold; D010655:Phenylenediamines / Q000737:chemistry; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry
D010655:Phenylenediamines; D011108:Polymers; D006046:Gold; C034193:1,2-diaminobenzene; D005947:Glucose