Hepatocarcinogenesis inhibition by caffeine in ACI rats treated with 2-acetylaminofluorene.
The inhibitory effects of caffeine have been demonstrated on the development of various organs in animals. The purpose of the present study was to examine the inhibitory effect of caffeine on hepatocarcinogenesis and to determine the responsive dose of caffeine on hepatocarcinogenesis in young male ACI rats. Animals given a diet containing 2-acetylaminofluorene (2-AAF) for 12 weeks and then a basal diet and tap water containing caffeine for 18 weeks showed statistically significant decreases in the incidence, multiplicity (the number of hepatic tumors per rat) and histological grade compared with rats fed a diet containing carcinogen for 12 weeks followed by tap water alone. Dose-dependent inhibition of hepatocarcinogenesis by caffeine was also seen. The inhibitory effect of caffeine on hepatocarcinogenesis in rats was found when caffeine was administered during the initiation phase.
Hosaka
S
S
The Second Department of Internal Medicine, Shinshu University School of Medicine, 3-1-1 Asahi Matsumoto, Nagano-ken 390-8621, Japan. hosakas@hsp.md.shinshu-u.ac.jp
Kawa
S
S
Aoki
Y
Y
Tanaka
E
E
Yoshizawa
K
K
Karasawa
Y
Y
Hosaka
N
N
Kiyosawa
K
K
D015073:2-Acetylaminofluorene / Q000633:toxicity*; D000818:Animals; D002110:Caffeine / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D002273:Carcinogens / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D004326:Drinking; D015994:Incidence; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced / Q000517:prevention & control*; D008297:Male; D051381:Rats; D011912:Rats, Inbred ACI; D013997:Time Factors
D002273:Carcinogens; D002110:Caffeine; D015073:2-Acetylaminofluorene