Differentiation of environmental and clinical isolates of Vibrio mimicus from Vibrio cholerae by multilocus enzyme electrophoresis.
In this study, we demonstrated that analyzed strains of Vibrio mimicus and Vibrio cholerae could be separated in two groups by using multilocus enzyme electrophoresis (MEE) data from 14 loci. We also showed that the combination of four enzymatic loci enables us to differentiate these two species. Our results showed that the ribosomal intergenic spacer regions PCR-mediated identification system failed, in some cases, to differentiate between V. mimicus and V. cholerae. On the other hand, MEE proved to be a powerful molecular tool for the discrimination of these two species even when atypical strains were analyzed.
Vieira
V V
VV
Department of Biochemistry and Molecular Biology, Instituto Oswaldo Cruz, Avenida Brasil 4365, Rio de Janeiro, Brazil CEP 21045-900. vvieira@gene.dbbm.fiocruz.br
Teixeira
L F
LF
Vicente
A C
AC
Momen
H
H
Salles
C A
CA
D015373:Bacterial Typing Techniques; D002771:Cholera / Q000382:microbiology; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D004783:Environmental Microbiology; D004798:Enzymes / Q000235:genetics; D006801:Humans; D014733:Vibrio / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D014735:Vibrio Infections / Q000382:microbiology; D014734:Vibrio cholerae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification
D004798:Enzymes