Glia-synapse interaction through Ca2+-permeable AMPA receptors in Bergmann glia.
Glial cells express a variety of neurotransmitter receptors. Notably, Bergmann glial cells in the cerebellum have Ca2+-permeable alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-type glutamate receptors (AMPARs) assembled without the GluR2 subunit. To elucidate the role of these Ca2+-permeable AMPARs, we converted them into Ca2+-impermeable receptors by adenoviral-mediated delivery of the GluR2 gene. This conversion retracted the glial processes ensheathing synapses on Purkinje cell dendritic spines and retarded the removal of synaptically released glutamate. Furthermore, it caused multiple innervation of Purkinje cells by the climbing fibers. Thus, the glial Ca2+-permeable AMPARs are indispensable for proper structural and functional relations between Bergmann glia and glutamatergic synapses.
Iino
M
M
Gunma University School of Medicine, Maebashi, Gunma 371-8511, Japan.
Goto
K
K
Kakegawa
W
W
Okado
H
H
Sudo
M
M
Ishiuchi
S
S
Miwa
A
A
Takayasu
Y
Y
Saito
I
I
Tsuzuki
K
K
Ozawa
S
S
D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000502:physiology*; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling; D019706:Excitatory Postsynaptic Potentials; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins; D066298:In Vitro Techniques; D008164:Luminescent Proteins / Q000235:genetics; D008564:Membrane Potentials; D018408:Patch-Clamp Techniques; D010539:Permeability; D011689:Purkinje Cells / Q000166:cytology / Q000502:physiology*; D051381:Rats; D018091:Receptors, AMPA / Q000235:genetics / Q000378:metabolism*; D013569:Synapses / Q000378:metabolism / Q000502:physiology*; D009435:Synaptic Transmission; D014162:Transfection; D018350:alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid / Q000494:pharmacology
D002118:Calcium; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins; D018091:Receptors, AMPA; D018350:alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid; C104722:glutamate receptor ionotropic, AMPA 2