Genetic diversity within the genus Trichinella as shown by cleavage fragment length polymorphism analysis.
The genetic diversity within the genus Trichinella was studied using cleavage fragment length polymorphism (CFLP) analysis. The CFLP method generates specific fingerprints based on single nucleotide mutations. By this method the amplified intergenic regions of the 5S rRNA genes of the eight different genotypes of Trichinella were analysed. The CFLP pattern of T. spiralis was completely different compared with the sylvatic species T. britovi, T. nativa, T. nelsoni, and the genotypes Trichinella T5, Trichinella T6 and Trichinella T8. The T. pseudospiralis intergenic region can be differentiated by size from the other species of Trichinella.
Rombout
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YB
National Institute of Public Health and the Environment (RIVM), Microbiological Laboratory for Health Protection, Antonie van Leeuwenhoeklaan 9, PO Box 1, 3720 BA Bilthoven, the Netherlands.
Bosch
S
S
Mandjes
B
B
Homan
W
W
van der Giessen
J W
JW
D000818:Animals; D016172:DNA Fingerprinting; D018509:DNA, Helminth / Q000235:genetics / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000235:genetics; D014644:Genetic Variation; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D015203:Reproducibility of Results; D014234:Trichinella / Q000235:genetics*
D018509:DNA, Helminth; D004275:DNA, Ribosomal