Group A streptococcal genotypes from pediatric throat isolates in Rome, Italy.
In a study assessing genetic diversity, 114 group A streptococcus (GAS) isolates were recovered from pediatric pharyngitis patients in Rome, Italy. These isolates comprised 22 different M protein gene (emm) sequence types, 14 of which were associated with a distinct serum opacity factor/fibronectin binding protein gene (sof) sequence type. Isolates with the same emm gene sequence type generally shared a highly conserved chromosomal macrorestriction profile. In three instances, isolates with dissimilar macrorestriction profiles had identical emm types; in each of these cases multilocus sequence typing revealed that isolates with the same emm type were clones having the same allelic profiles. Ninety-eight percent of the pharyngeal isolates had emm types previously found to be highly associated with mga locus gene patterns commonly found in pharyngeal GAS isolates.
Dicuonzo
G
G
Department of Medicine and Microbiology, Università Campus Bio-Medico, Rome, Italy.
Gherardi
G
G
Lorino
G
G
Angeletti
S
S
De Cesaris
M
M
Fiscarelli
E
E
Bessen
D E
DE
Beall
B
B
D000293:Adolescent; D000328:Adult; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D002352:Carrier Proteins / Q000235:genetics; D002648:Child; D002675:Child, Preschool; D016521:Electrophoresis, Gel, Pulsed-Field; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007558:Italy; D008297:Male; D010447:Peptide Hydrolases / Q000235:genetics; D010612:Pharyngitis / Q000382:microbiology*; D010614:Pharynx / Q000382:microbiology*; D012150:Polymorphism, Restriction Fragment Length; D013290:Streptococcal Infections / Q000382:microbiology*; D013297:Streptococcus pyogenes / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification
D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D002352:Carrier Proteins; C021908:opacity factor; C014689:streptococcal M protein; D010447:Peptide Hydrolases