The proliferative response of p53 knock-out mouse-derived vascular smooth muscle cell line, P53LMAC01, to PDGF, when compared with human aortic smooth muscle cells.
To develop an in vitro experimental model of vascular smooth muscle cell hyperplasia, a major feature in chronic cardiac rejection, we studied a novel vascular smooth muscle cell line, P53LMAC01 (AC01), which was established from aortic smooth muscles of p53 knock-out mice, to determine its response to a platelet-derived growth factor (PDGF) and to Cyclosporin A (CsA). The responses were compared with those of human aortic smooth muscle cells (AOSMC). The AC01 exhibited a distinct proliferative response to PDGF similar to that of AOSMC under serum-free conditions. 10 ng/ml of PDGF-BB increased by a factor of 4.5 and PDGF-AB doubled the thymidine uptake, but PDGF-AA caused only a slight increase. The proliferation was markedly inhibited by 10(-6) M of CsA but less affected by 10(-7) M. These results indicate that the AC01 cell line could provide a convenient experimental system for investigating chronic rejection in vitro and that the system might work as a screening model of agents for treating transplant-related arteriosclerosis.
Lu
H
H
Department of Experimental Surgery and Bioengineering, National, Children's Medical Research Cente, Toky, Japan.
Enosawa
S
S
Ohmi
K
K
Suzuki
S
S
D000818:Animals; D001011:Aorta / Q000166:cytology*; D001161:Arteriosclerosis / Q000473:pathology; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line / Q000187:drug effects; D016895:Culture Media, Serum-Free / Q000494:pharmacology; D016572:Cyclosporine / Q000494:pharmacology; D004261:DNA Replication / Q000187:drug effects; D016158:Genes, p53; D006084:Graft Rejection / Q000473:pathology; D016027:Heart Transplantation / Q000276:immunology; D006801:Humans; D006965:Hyperplasia; D051379:Mice; D018345:Mice, Knockout; D023421:Models, Animal; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects*; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology*; D013045:Species Specificity; D016159:Tumor Suppressor Protein p53 / Q000172:deficiency / Q000502:physiology*
D016895:Culture Media, Serum-Free; D010982:Platelet-Derived Growth Factor; D016159:Tumor Suppressor Protein p53; C075278:platelet-derived growth factor A; C095477:platelet-derived growth factor AB; D016572:Cyclosporine