title: Differential expression and function of the Drosophila Pax6 genes eyeless and twin of eyeless in embryonic central nervous system development.
abstract: We analyzed the expression and function of eyeless (ey) and twin of eyeless (toy) in the embryonic central nervous system (CNS) of Drosophila. Both genes are differentially expressed in specific neuronal subsets (but not in glia) in every CNS neuromere, and in the brain, specific cell populations co-expressing both proteins define a longitudinal domain which is intercalated between broad exclusive expression domains of ey and toy. Studies of genetic null alleles and dsRNA interference did not reveal any gross neuroanatomical effects of ey, toy, or ey/toy elimination in the embryonic CNS. In contrast, targeted misexpression of ey, but not of toy, resulted in profound axonal abnormalities in the embryonic ventral nerve cord and brain.
authors.affiliation: Institute of Zoology, Biocenter/Pharmacenter, University of Basel, Klingelbergstrasse 50, CH-4056 Basel, Switzerland.
authors.forename: L
authors.initials: L
authors.lastname: Kammermeier
authors.forename: R
authors.initials: R
authors.lastname: Leemans
authors.forename: F
authors.initials: F
authors.lastname: Hirth
authors.forename: S
authors.initials: S
authors.lastname: Flister
authors.forename: U
authors.initials: U
authors.lastname: Wenger
authors.forename: U
authors.initials: U
authors.lastname: Walldorf
authors.forename: W J
authors.initials: WJ
authors.lastname: Gehring
authors.forename: H
authors.initials: H
authors.lastname: Reichert
mesh_terms: D000483:Alleles; D000818:Animals; D002490:Central Nervous System / Q000196:embryology*; D003433:Crosses, Genetic; D004268:DNA-Binding Proteins / Q000096:biosynthesis* / Q000502:physiology*; D004330:Drosophila / Q000196:embryology*; D029721:Drosophila Proteins; D005136:Eye Proteins; D018398:Homeodomain Proteins / Q000096:biosynthesis* / Q000502:physiology*; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D018613:Microscopy, Confocal; D000071841:PAX6 Transcription Factor; D051761:Paired Box Transcription Factors; D017434:Protein Structure, Tertiary; D012330:RNA, Double-Stranded / Q000378:metabolism; D012097:Repressor Proteins; D014018:Tissue Distribution; D015534:Trans-Activators / Q000096:biosynthesis* / Q000502:physiology*
chemical_list: D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D005136:Eye Proteins; D018398:Homeodomain Proteins; D000071841:PAX6 Transcription Factor; D051761:Paired Box Transcription Factors; D012330:RNA, Double-Stranded; D012097:Repressor Proteins; D015534:Trans-Activators; C113278:ey protein, Drosophila; C118464:toy protein, Drosophila