A fucan from the brown seaweed Spatoglossum schröederi inhibits Chinese hamster ovary cell adhesion to several extracellular matrix proteins.
Fucans, a family of sulfated polysaccharides present in brown seaweed, have several biological activities. Their use as drugs would offer the advantage of no potential risk of contamination with viruses or particles such as prions. A fucan prepared from Spatoglossum schröederi was tested as a possible inhibitor of cell-matrix interactions using wild-type Chinese hamster ovary cells (CHO-K1) and the mutant type deficient in xylosyltransferase (CHO-745). The effect of this polymer on adhesion properties with specific extracellular matrix components was studied using several matrix proteins as substrates for cell attachment. Treatment with the polymer inhibited the adhesion of fibronectin to both CHO-K1 (2 x 10(5)) and CHO-745 (2 x 10(5) and 5 x 10(5)) cells. No effect was detected with laminin, using the two cell types. On the other hand, adhesion to vitronectin was inhibited in CHO-K1 cells and adhesion to type I collagen was inhibited in CHO-745 cells. In spite of this inhibition, the fucan did not affect either cell proliferation or cell cycle. These results demonstrate that this polymer is a new anti-adhesive compound with potential pharmacological applications.
Rocha
H A
HA
Departamento de Bioquímica, Universidade Federal do Rio Grande do Norte, Natal, RN, Brasil.
Franco
C R
CR
Trindade
E S
ES
Carvalho
L C
LC
Veiga
S S
SS
Leite
E L
EL
Dietrich
C P
CP
Nader
H B
HB
D000818:Animals; D000925:Anticoagulants / Q000737:chemistry* / Q000494:pharmacology; D016466:CHO Cells / Q000187:drug effects; D002448:Cell Adhesion / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D006224:Cricetinae; D016326:Extracellular Matrix Proteins / Q000037:antagonists & inhibitors / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology; D012624:Seaweed / Q000737:chemistry*; D013936:Thymidine / Q000378:metabolism
D000925:Anticoagulants; D016326:Extracellular Matrix Proteins; D011134:Polysaccharides; D013936:Thymidine