Localization of mouse CLC-6 and CLC-7 mRNA and their functional complementation of yeast CLC gene mutant.
CLC-6 and CLC-7 belong to the family of voltage-dependent chloride channels. To learn more about the in vivo roles of CLC-6 and CLC-7, we performed in situ hybridization of these CLC channels in various mouse organs. Mouse CLC-6 (mCLC-6) was expressed in the peripheral region of seminiferous tubules in the testis, tracheal epithelium, epithelium of bronchioles, alveolar cells in the lung, acinar cells in the pancreas, and intestinal epithelium, but we could not detect signals from pancreatic islets. Mouse CLC-7 (mCLC-7) was expressed in neurons in the medulla oblongata, Purkinje cells in the cerebellum, proximal tubules in the kidney, and hepatocytes in the liver. The distribution of mCLC-6 and mCLC-7 were similar in the lung, pancreas, and testis. mCLC-6 functionally complemented the gef1 phenotype of a yeast strain in which a single CLC channel (GEF1) had been disrupted by homologous recombination. In contrast, mCLC-7 did not complement this gef1 phenotype. This study identified the cell types that express mCLC-6 and mCLC-7 in the mouse tissues, and the complementation assay suggested that mCLC-6 functions as an intracellular chloride channel.
Kida
Y
Y
Graduate School, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8519, Japan.
Uchida
S
S
Miyazaki
H
H
Sasaki
S
S
Marumo
F
F
D000818:Animals; D018118:Chloride Channels / Q000235:genetics / Q000378:metabolism*; D005656:Fungal Proteins / Q000235:genetics*; D005816:Genetic Complementation Test; D017403:In Situ Hybridization; D008297:Male; D008565:Membrane Proteins / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D009154:Mutation; D009928:Organ Specificity; D012333:RNA, Messenger / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins
D018118:Chloride Channels; C490800:Clcn6 protein, mouse; C490803:Clcn7 protein, mouse; D005656:Fungal Proteins; C089713:GEF1 protein, S cerevisiae; D008565:Membrane Proteins; D012333:RNA, Messenger; D029701:Saccharomyces cerevisiae Proteins