[The analysis of fatty acid composition in syncytiotrophoblast plasma membranes of human placenta].
The fatty acids were determined in syncytiotrophoblast plasma membranes of the eighteen human placenta from the normal pregnant women. With the method of Landon, the fat was purified in syncytiotrophoblast plasma membranes, which was extracted from the placenta by using the procedure of Whitsett. The methylated fatty acids were separated with gas chromatography in which a glass column packed with DEGS was used. In comparing the retention time of the sample with the standards and referring to the results of GC/MS, thirteen peaks were identified and quantified. The area normalization method was used to determine the distribution, the compositions and contents of fatty acids in syncytiotrophoblast plasma membranes. It is shown that the amount of identified fatty acids attains 74.1% of total peak area, in which the amount of saturated fatty acids was 30.64%, and the unsaturated was 43.46%.
Sang
C
C
First Hospital Affiliated Dalian Medical University, Dalian, 116011.
Yin
X
X
Man
H
H
Chen
J
J
D002462:Cell Membrane / Q000737:chemistry; D002849:Chromatography, Gas; D005227:Fatty Acids / Q000032:analysis*; D005260:Female; D006801:Humans; D010920:Placenta / Q000737:chemistry / Q000166:cytology*; D011247:Pregnancy; D014327:Trophoblasts / Q000737:chemistry*
D005227:Fatty Acids