Enhanced production of IL-1beta and IL-6 following endotoxin challenge in rats with dietary magnesium deficiency.
Serum IL-1beta, IL-6 and TNFalpha were not detected in control and Mg-deficient rats. These three cytokine levels in serum were increased after endotoxin challenge (1 mg/kg., i.p.), and the increase of IL-1beta and IL-6, but not TNFalpha, was significantly larger in Mg-deficient rats than in controls. Levels of mRNA for IL-1beta, IL-6 and TNFalpha in alveolar macrophages showed a tendency to decrease during Mg deficiency, but the levels of IL-1beta and TNFalpha mRNAs after endotoxin challenge were higher in Mg-deficient rats than in controls. These results suggest that the increased synthesis of cytokines by alveolar macrophages might contribute, in part, to high sensitivity to endotoxin during Mg deficiency.
Nakagawa
M
M
Department of Veterinary Pharmacology, Faculty of Agriculture, Kagoshima University, Japan.
Oono
H
H
Nishio
A
A
D000818:Animals; D007375:Interleukin-1 / Q000096:biosynthesis* / Q000097:blood / Q000235:genetics; D015850:Interleukin-6 / Q000096:biosynthesis* / Q000097:blood / Q000235:genetics; D008070:Lipopolysaccharides / Q000494:pharmacology*; D016676:Macrophages, Alveolar / Q000187:drug effects / Q000378:metabolism*; D008274:Magnesium / Q000097:blood; D008275:Magnesium Deficiency / Q000097:blood* / Q000473:pathology; D008297:Male; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism
D007375:Interleukin-1; D015850:Interleukin-6; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D008274:Magnesium