EGR-1, a UV-inducible gene in p53(-/-) mouse cells.
Changes in gene expression were examined in p53(-/-) and p53(+/+) mouse cells after ultraviolet (UV) irradiation. Differential display was used to identify differentially expressed gene(s) in UV-treated p53(-/-) and p53(+/+) cells. One of the differentially expressed genes was EGR-1 (early growth response gene-1), which was shown to be induced only in p53(-/-) cells. The induction of this gene by UV was detected as early as 0.5 h, peaked at 2 h, and returned to normal levels by 4 h. De novo protein synthesis was not required for UV-induced EGR-1 expression in p53(-/-) cells. Pretreatment of p53(-/-) cells with suramin, an inhibitor of growth factor receptors, completely suppressed UV-induced EGR-1 expression, suggesting that the induction may be mediated via the growth factor receptors. The presence of wild-type p53 suppressed the induction of EGR-1 after UV treatment. Overexpression of EGR-1 promoted the UV-induced transformation in p53(+/+) cells, but not in p53(-/-) cells. These data suggested that EGR-1 may be an important player in the UV responses of mammalian cells and may influence UV-induced transformation.
Zhang
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Laboratory for Cancer Research, College of Pharmacy, Rutgers, the State University of New Jersey, 164 Frelinghuysen Road, Piscataway, New Jersey 08854, USA.
Chen
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D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology; D002461:Cell Line, Transformed / Q000187:drug effects / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D051766:Early Growth Response Protein 1; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D017874:Immediate-Early Proteins; D051379:Mice; D018345:Mice, Knockout / Q000235:genetics / Q000378:metabolism; D009369:Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology; D017978:Receptors, Growth Factor / Q000187:drug effects / Q000378:metabolism; D013498:Suramin / Q000494:pharmacology; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000172:deficiency* / Q000235:genetics*; D014466:Ultraviolet Rays / Q000009:adverse effects*
D000970:Antineoplastic Agents; D004268:DNA-Binding Proteins; D051766:Early Growth Response Protein 1; C486109:Egr1 protein, mouse; D017874:Immediate-Early Proteins; D017978:Receptors, Growth Factor; D014157:Transcription Factors; D016159:Tumor Suppressor Protein p53; D013498:Suramin