title: Expedient liquid chromatographic method with fluorescence detection for montelukast sodium in micro-samples of plasma.
abstract: This study describes an expedient assay for the analysis of the asthma medication, montelukast sodium (Singulair, MK-0476), in human plasma samples. After a simple extraction of the plasma, the drug and internal standard, quinine bisulfate, were measured by HPLC. The chromatographic system consisted of a single pump, a refrigerated autosampler, a C8 4-microm particle size radial compression cartridge at 40 degrees C and a fluorescence detector with the excitation and emission wavelengths set at 350 and 400 nm, respectively. The mobile phase which was delivered at 1.0 ml/min, was prepared by adding 200 ml of 0.025 M sodium acetate, pH adjusted to 4.0 with acetic acid, to 800 ml of acetonitrile, with 50 microl triethylamine. With a run time of only 10 min per sample, this assay had an overall recovery of >97% with a detection limit of 1 ng/ml. The inter- and intra-run relative standard deviations at 0.05, 0.2 and 1.0 microg/ml were all <9.2%, while the analytical recovery at the same concentrations were within 7.7% of the amount added.
authors.affiliation: Biological and Medical Research Department, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia. sameer@kfshrc.edu.sa
authors.forename: S
authors.initials: S
authors.lastname: Al-Rawithi
authors.forename: S
authors.initials: S
authors.lastname: Al-Gazlan
authors.forename: W
authors.initials: W
authors.lastname: Al-Ahmadi
authors.forename: I A
authors.initials: IA
authors.lastname: Alshowaier
authors.forename: A
authors.initials: A
authors.lastname: Yusuf
authors.forename: D A
authors.initials: DA
authors.lastname: Raines
mesh_terms: D000085:Acetates / Q000097:blood*; D018927:Anti-Asthmatic Agents / Q000097:blood*; D001249:Asthma / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003521:Cyclopropanes; D005453:Fluorescence; D006801:Humans; D008027:Light; D011786:Quality Control; D011804:Quinolines / Q000097:blood*; D012015:Reference Standards; D013440:Sulfides
chemical_list: D000085:Acetates; D018927:Anti-Asthmatic Agents; D003521:Cyclopropanes; D011804:Quinolines; D013440:Sulfides; C093875:montelukast