Reconstruction of ureteral defects with microvascular vein grafts in a rat model.
This study was undertaken to test the performance of an autologous vein graft as a ureteral replacement in the rat model. Twenty-six rats were divided into three groups. In Group 1 (n = 10), the animals had a 3-mm segment of the ipsilateral ureter excised and the ureteral defect repaired, using a superficial epigastric vein graft. In Group 2 (n = 10), the same ureteral defect was created and again repaired, using a superficial epigastric vein graft, with the addition of a Silastic stent. The control, Group 3 (n = 6), had the ureter transected and repaired solely by means of primary anastomosis. Animals from each group underwent urography and were sacrificed for histology at three different postoperative intervals: 1, 4, and 12 weeks. Postoperative urography results showed normal renal function in the animals with ureteral reconstruction using vein grafting aided by a stent, as well as in those with primary ureteral anastomosis. No renal function return was seen in the animals with ureteral reconstruction by vein grafting alone, without stent support. Histologically, a progressive loss of the vascular endothelium, and replacement with the urothelium typical of the ureter, was seen in the stented vein grafts. Severe ureteral obstruction at the proximal site of the graft and hydronephrosis were seen in the vein-graft group without stenting. This study demonstrates that autologous vein grafts can be used successfully to correct a ureteral deficit, contingent on accurate microsurgical technique and immediate stenting.
Zhang
F
F
Division of Plastic Surgery, University of Mississippi Medical Center, Jackson 39216, USA.
Sones
W D
WD
Guo
M
M
Xu
X Z
XZ
Buncke
H J
HJ
Dorsett-Martin
W
W
Lineaweaver
W C
WC
D000714:Anastomosis, Surgical; D000818:Animals; D004730:Endothelium, Vascular / Q000473:pathology; D008297:Male; D008866:Microsurgery; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D019651:Plastic Surgery Procedures; D015607:Stents; D014182:Transplantation, Autologous; D014513:Ureter / Q000293:injuries* / Q000473:pathology; D014515:Ureteral Diseases / Q000473:pathology / Q000601:surgery*; D014680:Veins / Q000637:transplantation*