title: Glucosyltransferase inactivation reduces dental caries.
abstract: Dental caries has been an intractable disease in spite of intense dental research. The metabolic acids produced by mutans streptococci demineralize the tooth surface and lead to dental caries. The enzyme glucosyltransferase (GTF) produced by mutans streptococci is the key factor in this process. Oral bacterial GTFs use sucrose as a substrate in synthesis of either water-soluble or insoluble glucans. In this investigation, kinetic studies with divalent metal ions revealed their strong binding affinity to GTF. The metal ions also proved to be strong inhibitors of the enzyme. Here we describe a simple method of inactivating the enzyme that actively participates in dental caries by taking advantage of a Fenton reaction which requires metal ions such as iron or copper and peroxide. The hydroxyl radical ions produced via the Fenton reaction inactivate GTF, a factor in the production of dental caries.
authors.affiliation: Department of Basic Sciences, School of Dentistry, University of Southern California, Los Angeles 90089-0641, USA.
authors.forename: K S
authors.initials: KS
authors.lastname: Devulapalle
authors.forename: G
authors.initials: G
authors.lastname: Mooser
mesh_terms: D001667:Binding, Competitive; D003731:Dental Caries / Q000201:enzymology* / Q000517:prevention & control*; D005296:Ferrous Compounds / Q000737:chemistry / Q000494:pharmacology*; D005964:Glucosyltransferases / Q000037:antagonists & inhibitors*; D017665:Hydroxyl Radical / Q000737:chemistry / Q000378:metabolism; D007700:Kinetics; D012044:Regression Analysis; D013295:Streptococcus mutans / Q000201:enzymology*
chemical_list: D005296:Ferrous Compounds; D017665:Hydroxyl Radical; C020748:ferrous sulfate; D005964:Glucosyltransferases