Involvement of aspirin-sensitive oxylipins in vulvovaginal candidiasis.
3(R)-Hydroxyoxylipins are produced via an aspirin-sensitive pathway in Candida albicans, an abundant pathogen in vulvovaginal candidiasis. In the present study, we have investigated the effect of aspirin on vaginal isolates of C. albicans from patients with recurrent candidiasis. Aspirin alone and with clotrimazole, a commonly used drug, strongly suppressed growth of C. albicans. 3(R)-Hydroxyoxylipins, which were selectively located in hyphae and other filamentous structures, but not in free blastospores, were almost totally suppressed by aspirin. Moreover, C. albicans stimulated prostaglandin E(2) (PGE(2)) production in HeLa cells. PGE(2) is a stimulus for germ tube formation in C. albicans. We conclude therefore that the administration of aspirin should be beneficial in the treatment of vulvovaginal candidiasis by dual ways: (i) by inhibition of 3(R)-hydroxyoxylipin formation, and (ii) by inhibition of PGE(2) formation in the infected host tissue.
Deva
R
R
Eicosanoid Research Division, Department of Gynecology and Obstetrics, University Medicine Center Benjamin Franklin, Free University Berlin, Hindenburgdamm 30, D-12200 Berlin, Germany.
Ciccoli
R
R
Kock
L
L
Nigam
S
S
D000935:Antifungal Agents / Q000494:pharmacology; D001095:Arachidonic Acids / Q000494:pharmacology*; D001241:Aspirin / Q000494:pharmacology*; D002176:Candida albicans / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D002181:Candidiasis, Vulvovaginal / Q000382:microbiology*; D003022:Clotrimazole / Q000494:pharmacology; D015232:Dinoprostone / Q000096:biosynthesis; D005260:Female; D006367:HeLa Cells; D006801:Humans; D008856:Microscopy, Fluorescence; D012008:Recurrence
D000935:Antifungal Agents; D001095:Arachidonic Acids; D003022:Clotrimazole; D015232:Dinoprostone; D001241:Aspirin