title: Identification of the regions of Fv1 necessary for murine leukemia virus restriction.
abstract: The Fv1 gene restricts murine leukemia virus replication via an interaction with the viral capsid protein. To study this interaction, a number of mutations, including a series of N-terminal and C-terminal deletions, internal deletions, and a number of single-amino-acid substitutions, were introduced into the n and b alleles of the Fv1 gene and the effects of these changes on virus restriction were measured. A significant fraction of the Fv1 protein was not required for restriction; however, retention of an intact major homology region as well as of domains toward the N and C termini was essential. Binding specificity appeared to be a combinatorial property of a number of residues within the C-terminal portion of Fv1.
authors.affiliation: Division of Virology, National Institute for Medical Research, London NW7 1AA, United Kingdom.
authors.forename: K N
authors.initials: KN
authors.lastname: Bishop
authors.forename: M
authors.initials: M
authors.lastname: Bock
authors.forename: G
authors.initials: G
authors.lastname: Towers
authors.forename: J P
authors.initials: JP
authors.lastname: Stoye
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D016384:Consensus Sequence; D017353:Gene Deletion; D009052:Leukemia Virus, Murine / Q000235:genetics* / Q000472:pathogenicity; D007942:Leukemia, Experimental / Q000821:virology; D051379:Mice; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D011506:Proteins / Q000737:chemistry / Q000235:genetics*; D015183:Restriction Mapping; D012192:Retroviridae Infections / Q000821:virology; D014412:Tumor Virus Infections / Q000821:virology; D014774:Virulence; D014779:Virus Replication
chemical_list: C103189:Fv1 protein, mouse; D011506:Proteins