[Zinc modulation of GABA receptors expressed in Xenopus oocytes injected with carp brain poly(A)+ RNA].
Two types of GABA receptors, GABAA receptors (approximately 85%) and GABAC-like receptors (approximately 15%), were expressed in Xenopus oocytes after injection of Poly(A)+ RNA with carp (Carassius carassius) brain. Using voltage-clamp technique and pharmacological methods, the effects of zinc on the two receptors were studied. It was found that zinc modulation of both GABAA and GABAC-like receptors by zinc was inhibitory and reversible with the former being stronger than the latter as indicated by a value of IC50 of 48.4 +/- 10.1 mumol/L against 255.6 +/- 21.5 mumol/L.
Bai
X C
XC
Shanghai Institute of Physiology, Chinese Academy of Sciences, Shanghai 200031.
Bao
Y D
YD
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002347:Carps; D005260:Female; D009865:Oocytes / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D018079:Receptors, GABA / Q000096:biosynthesis*; D018080:Receptors, GABA-B / Q000096:biosynthesis*; D014162:Transfection; D014982:Xenopus laevis; D015032:Zinc / Q000494:pharmacology*
C087200:GABA-C receptor; D012333:RNA, Messenger; D018079:Receptors, GABA; D018080:Receptors, GABA-B; D015032:Zinc