title: [Analysis of paracetamol, caffeine and chlorphenamine maleate in suxiaoshangfeng capsules by capillary gas chromatography].
abstract: A simple and rapid method for simultaneous determination of paracetamol, caffeine and chlorphenamine maleate in Suxiaoshangfeng capsule by capillary gas chromatography with n-hexadecane as internal standard has been reported. In the capsule, the three components can be separated on a 5% Ph-Me-Silicone column (2.65 microns x 0.53 mm x 10 m), with programmed column temperature from 180 degrees C to 230 degrees C at 8 degrees C/min, hold 6 min and then 230 degrees C to 260 degrees C at 10 degrees C/min, hold 10 min. Both the injector and FID detetor temperatures were 280 degrees C. N2 flow rate was 3.5 mL/min and H2 35 mL/min. The injection volume was 1 uL with splitting ratio of 1/25. These components were identified by their retention times and quantitatively determined by their peak areas. The calibration curves were, r = 0.9996 for paracetamol, r = 0.9984 for caffeine and r = 0.9996 for chlorphenamine maleate. The linear ranges were 4.0-20 g/L, 0.15-0.75 g/L and 0.080-0.40 g/L, respectively. The average recoveries were 99.62% (RSD = 0.40%, n = 5), 96.46%. (RSD = 1.32%, n = 5) and 98.55% (RSD = 0.65%, n = 5), respectively.
authors.affiliation: Institute of Medicaments, Guangdong College of Pharmacy, Guangzhou, 510240.
authors.forename: X
authors.initials: X
authors.lastname: Guo
authors.forename: W
authors.initials: W
authors.lastname: Qian
authors.forename: C
authors.initials: C
authors.lastname: Yang
authors.forename: X
authors.initials: X
authors.lastname: Zhu
mesh_terms: D000082:Acetaminophen / Q000032:analysis*; D018712:Analgesics, Non-Narcotic / Q000737:chemistry*; D002110:Caffeine / Q000032:analysis*; D002214:Capsules; D002744:Chlorpheniramine / Q000032:analysis*; D002849:Chromatography, Gas; D004339:Drug Compounding; D004365:Drugs, Chinese Herbal / Q000737:chemistry*
chemical_list: D018712:Analgesics, Non-Narcotic; D002214:Capsules; D004365:Drugs, Chinese Herbal; D000082:Acetaminophen; D002110:Caffeine; D002744:Chlorpheniramine