title: RANTES production from CD4+ lymphocytes correlates with host genotype and rates of human immunodeficiency virus type 1 disease progression.
abstract: Several chemokine and chemokine receptor parameters were measured in peripheral blood mononuclear cells obtained from patients before they became infected with human immunodeficiency virus type 1 (HIV-1). After HIV-1 infection, the parameters were compared with plasma HIV-1 RNA levels and with rates of CD4(+) lymphocyte decline. Patients who were heterozygous for the Delta32CCR5 allele had significantly higher levels of RANTES production from their CD4(+) lymphocytes than did patients who did not carry the Delta32CCR5 allele (P=.01). Higher RANTES production levels from ex vivo-activated CD4(+)-enriched lymphocytes, but not CD8(+) lymphocytes, correlated with lower plasma HIV-1 RNA levels 9-12 months after infection (P= .01) and with slower rates of CD4(+) lymphocyte decline (P= .002). CCR5 expression levels on ex vivo-activated CD4(+) lymphocytes did not correlate with markers of disease progression. These results further support the hypothesis that chemokine production levels are associated with HIV-1 replication in vivo.
authors.affiliation: Department of Human Retrovirology, Academic Medical Center, Amsterdam, 1105 AZ, The Netherlands. w.a.paxton@amc.uva.nl
authors.forename: W A
authors.initials: WA
authors.lastname: Paxton
authors.forename: A U
authors.initials: AU
authors.lastname: Neumann
authors.forename: S
authors.initials: S
authors.lastname: Kang
authors.forename: L
authors.initials: L
authors.lastname: Deutch
authors.forename: R C
authors.initials: RC
authors.lastname: Brown
authors.forename: R A
authors.initials: RA
authors.lastname: Koup
authors.forename: S M
authors.initials: SM
authors.lastname: Wolinsky
mesh_terms: D000483:Alleles; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D018946:Chemokine CCL5 / Q000032:analysis / Q000096:biosynthesis*; D015331:Cohort Studies; D018450:Disease Progression; D005838:Genotype; D015658:HIV Infections / Q000235:genetics* / Q000276:immunology / Q000821:virology; D015497:HIV-1 / Q000472:pathogenicity; D006801:Humans; D008297:Male; D012333:RNA, Messenger / Q000032:analysis; D012367:RNA, Viral / Q000097:blood; D019713:Receptors, CCR5 / Q000096:biosynthesis / Q000235:genetics*; D015728:Receptors, HIV / Q000235:genetics; D019562:Viral Load
chemical_list: D018946:Chemokine CCL5; D012333:RNA, Messenger; D012367:RNA, Viral; D019713:Receptors, CCR5; D015728:Receptors, HIV