[A simple method of isolation and identification of P cells in rat sinoatrial node].
A method of isolating P cells of rat sinoatrial node by collagenase is described. The method is feasible for morphological and electrophysiological investigation, with the advantages of taking a relatively easy procedure and consuming a small amount of collagenase. The main features of action potentials of these isolated cells including clear diastolic automatic depolarization, mean maximal diastolic potential of -55 mV, mean peak potential value of 58 mV, and mean APD50 of 18 ms, were similar to those of P cells in situ.
Zeng
Y M
YM
Department of Physiology, Medical College, Shantou University, Shantou 515031.
Li
C
C
D000200:Action Potentials; D000818:Animals; D002469:Cell Separation / Q000379:methods*; D017364:Collagenases; D051381:Rats; D017207:Rats, Sprague-Dawley; D012849:Sinoatrial Node / Q000166:cytology*
D017364:Collagenases