Specific identification of Lactobacillus helveticus by PCR with pepC, pepN and htrA targeted primers.
Specific regions in three genes coding for aminopeptidases C and N, and a trypsin-like serine protease were selected as species-specific primer sequences for rapid and reliable identification of Lactobacillus helveticus strains. The PCR procedures carried out gave specific 524-, 726- and 918-bp amplificates, with DNA isolated from L. helveticus. No PCR product was generated for closely related bacteria. The amplification products were also screened for their species specificity in dot blot hybridization with representatives of the most closely related genera and species and a number of other bacterial species.
Fortina
M G
MG
Dipartimento di Scienze e Tecnologie Alimentari e Microbiologiche, Sezione di Microbiologia Industriale, Università degli Studi di Milano, Via Celoria 2, 20133, Milan, Italy. grazia.fortina@unimi.it
Ricci
G
G
Mora
D
D
Parini
C
C
Manachini
P L
PL
D000626:Aminopeptidases; D001426:Bacterial Proteins / Q000235:genetics*; D017931:DNA Primers; D015342:DNA Probes; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D006360:Heat-Shock Proteins; D007778:Lactobacillus / Q000145:classification* / Q000235:genetics*; D033903:Periplasmic Proteins; D016133:Polymerase Chain Reaction; D012697:Serine Endopeptidases / Q000235:genetics*; D013045:Species Specificity
D001426:Bacterial Proteins; D017931:DNA Primers; D015342:DNA Probes; D004269:DNA, Bacterial; D006360:Heat-Shock Proteins; D033903:Periplasmic Proteins; C075633:pepN protein, Bacteria; D000626:Aminopeptidases; C061682:DegP protease; C080203:PEPC proteinase; D012697:Serine Endopeptidases