Stimulation of myelopoiesis in Listeria monocytogenes-infected mice by an aggregated polymer isolated from Aspergillus oryzae.
In this work, we investigated the effects of the proteic aggregated polymer of magnesium ammonium phospholinoleate-palmitoleate anhydride (MAPA) isolated from Aspergillus oryzae on the growth and differentiation of bone marrow granulocyte-macrophage progenitor cells (CFU-GM) in Listeriamonocytogenes-infected mice. A significant reduction in the CFU-GM number was observed in the initial phase of infection with a sublethal dose of Listeria. Treatment of mice with 0.5, 2.0 and 5.0 mg/kg MAPA for 7 days prior to infection significantly stimulated myelopoiesis in a dose-dependent manner. Moreover, treatment with 0.5 and 5.0 mg/kg MAPA resulted in 30% and 40% cures of mice lethally infected with Listeria, respectively. MAPA added directly to the culture dishes hardly affected colony formation by bone marrow cells, suggesting an indirect effect ofthis compound on myelopoiesis in vivo. In summary, the data show that MAPA can modulate the CFU-GM generation and antibacterial resistance in listeriosis. As the ability of hematopoietic tissues to produce phagocytes is of particular significance to mediate resistance to Listeria, the promotion of bone marrow CFU-GM by MAPA may contribute to a rapid restoration of phagocyte numbers in infected sites, thus mitigating the course of infection.
de Melo
A
A
Department of Pharmacology and Hemocentre, Faculdade de Ciências Médicas, Universidade Estadual de Campinas, SP, Brazil.
Justo
G Z
GZ
de Souza Queiroz
M L
ML
D000818:Animals; D001236:Aspergillus oryzae / Q000737:chemistry*; D001854:Bone Marrow Cells / Q000187:drug effects; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D007279:Injections, Subcutaneous; D019891:Leukopoiesis / Q000187:drug effects*; D008088:Listeriosis / Q000473:pathology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011108:Polymers / Q000627:therapeutic use*; D011994:Recombinant Proteins; D013234:Stem Cells / Q000187:drug effects; D016019:Survival Analysis
D011108:Polymers; D011994:Recombinant Proteins; D016178:Granulocyte-Macrophage Colony-Stimulating Factor