Cosuppression of the alpha subunits of beta-conglycinin in transgenic soybean seeds induces the formation of endoplasmic reticulum-derived protein bodies.
The expression of the alpha and alpha' subunits of beta-conglycinin was suppressed by sequence-mediated gene silencing in transgenic soybean seed. The resulting seeds had similar total oil and protein content and ratio compared with the parent line. The decrease in beta-conglycinin protein was apparently compensated by an increased accumulation of glycinin. In addition, proglycinin, the precursor of glycinin, was detected as a prominent polypeptide band in the protein profile of the transgenic seed extract. Electron microscopic analysis and immunocytochemistry of maturing transgenic soybean seeds indicated that the process of storage protein accumulation was altered in the transgenic line. In normal soybeans, the storage proteins are deposited in pre-existing vacuoles by Golgi-derived vesicles. In contrast, in transgenic seed with reduced beta-conglycinin levels, endoplasmic reticulum (ER)-derived vesicles were observed that resembled precursor accumulating-vesicles of pumpkin seeds and the protein bodies accumulated by cereal seeds. Their ER-derived membrane of the novel vesicles did not contain the protein storage vacuole tonoplast-specific protein alpha-TIP, and the sequestered polypeptides did not contain complex glycans, indicating a preGolgi and nonvacuolar nature. Glycinin was identified as a major component of these novel protein bodies and its diversion from normal storage protein trafficking appears to be related to the proglycinin buildup in the transgenic seed. The stable accumulation of proteins in a protein body compartment instead of vacuolar accumulation of proteins may provide an alternative intracellular site to sequester proteins when soybeans are used as protein factories.
Kinney
A J
AJ
DuPont Nutrition and Health, P.O. Box 80402, Experimental Station, Wilmington, Delaware 19880-0402, USA.
Jung
R
R
Herman
E M
EM
D052179:Antigens, Plant; D004721:Endoplasmic Reticulum / Q000502:physiology* / Q000648:ultrastructure; D020868:Gene Silencing; D005916:Globulins / Q000235:genetics* / Q000378:metabolism; D008565:Membrane Proteins / Q000032:analysis; D008954:Models, Biological; D015388:Organelles / Q000502:physiology* / Q000648:ultrastructure; D010940:Plant Proteins / Q000032:analysis / Q000235:genetics*; D030821:Plants, Genetically Modified; D011134:Polysaccharides / Q000032:analysis; D011498:Protein Precursors / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D021122:Protein Subunits; D055314:Seed Storage Proteins; D012639:Seeds / Q000502:physiology / Q000648:ultrastructure; D030262:Soybean Proteins; D013025:Glycine max / Q000502:physiology* / Q000648:ultrastructure; D013489:Suppression, Genetic; D014617:Vacuoles / Q000201:enzymology
D052179:Antigens, Plant; D005916:Globulins; D008565:Membrane Proteins; D010940:Plant Proteins; D011134:Polysaccharides; D011498:Protein Precursors; D021122:Protein Subunits; D055314:Seed Storage Proteins; D030262:Soybean Proteins; C013894:beta-conglycinin protein, Glycine max; C078044:alpha-TIP protein, Phaseolus vulgaris; C011474:glycinin