Cloning of new members of heat shock protein HSP101 gene family in wheat (Triticum aestivum (L.) Moench) inducible by heat, dehydration, and ABA(1).
We have cloned two cDNAs, TaHSP101B and TaHSP101C, encoding two heat stress-inducible members of HSP101/ClpB family in bread wheat (Triticum aestivum (L.) Moench.). Proteins encoded by these cDNAs are highly similar at the primary sequence level and diverged from the previously reported TaHSP101 (designated TaHSP101A) both in the consensus ATP/GTP-binding region II and in the carboxy terminal region. The HSP101 gene was determined to be a single copy gene or a member of a small gene family in hexaploid wheat. Messages encoding HSP101 proteins were inducible by heat stress treatments in both wheat leaves and roots. Accumulation of the TaHSP101C mRNA was less abundant than that of TaHSP101B mRNA. We are showing for the first time that in addition to heat stress, expression of HSP101 mRNAs in wheat leaves was induced by a 2-h dehydration and a treatment with 5x10(-5)M ABA, but not affected by chilling or wounding, indicating that HSP101 proteins may be involved in both heat and drought responses in wheat.
Campbell
J L
JL
Department of Plant and Soil Science, Texas Tech University, Lubbock, TX 79409, USA.
Klueva
N Y
NY
Zheng
H G
HG
Nieto-Sotelo
J
J
Ho
T D
TD
Nguyen
H T
HT
D000040:Abscisic Acid / Q000494:pharmacology; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D016384:Consensus Sequence; D018076:DNA, Complementary / Q000096:biosynthesis / Q000737:chemistry; D049071:Endopeptidase Clp; D029968:Escherichia coli Proteins; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant; D006360:Heat-Shock Proteins / Q000737:chemistry / Q000235:genetics*; D006358:Hot Temperature; D008969:Molecular Sequence Data; D010802:Phylogeny; D018515:Plant Leaves / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D018517:Plant Roots / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D016415:Sequence Alignment; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics*; D014908:Triticum / Q000235:genetics*
D018076:DNA, Complementary; D029968:Escherichia coli Proteins; C115569:HSP101 protein, plant; D006360:Heat-Shock Proteins; D010940:Plant Proteins; D012333:RNA, Messenger; D014157:Transcription Factors; D000040:Abscisic Acid; D049071:Endopeptidase Clp; C069775:ClpB protein, E coli