title: Acyl-CoA-binding protein in the armadillo Harderian gland: its primary structure and possible role in lipid secretion.
abstract: Similar to those of other species, the Harderian glands of armadillo produce an abundant lipid secretion, most of which is composed of 1-alkyl-2,3-diacylglycerol. Biosynthesis of this component is apparently performed with the participation of one cytosolic pool of acyl-CoA and another of free fatty acids. The acyl-CoA-binding protein (ACBP) is present at a concentration at least 7-fold that of the heart-type fatty acid-binding protein (H-FABP), though lower than that in other armadillo organs such as liver and brain. The ACBP complete amino acid sequence was determined by Edman degradation of peptides generated by cleavage of the protein with cyanogen bromide, endopeptidase Glu-C, and trypsin. ACBP consists of 86 residues and has a calculated molecular mass of 9783 Da, taking into account that an acetyl group is blocking the N-terminus. Identity percentages between armadillo Harderian gland ACBP and other known ACBPs show that the protein belongs to the liver-specific ACBP isoform (L-ACBP). The fact that the ACBP concentration is higher than that of FABP suggests that the Harderian gland is able to store acyl-CoA amounts in ACBP larger than those of fatty acids in H-FABP for 1-alkyl-2,3-diacylglycerol synthesis.
authors.affiliation: Instituto de Química y Fisicoquímica Biológicas, CONICET-UBA, Buenos Aires, Argentina.
authors.forename: B M
authors.initials: BM
authors.lastname: Cavagnari
authors.forename: N
authors.initials: N
authors.lastname: Sterin-Speziale
authors.forename: J M
authors.initials: JM
authors.lastname: Affanni
authors.forename: J
authors.initials: J
authors.lastname: Knudsen
authors.forename: J A
authors.initials: JA
authors.lastname: Santomé
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001135:Armadillos / Q000378:metabolism*; D002352:Carrier Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000502:physiology; D002850:Chromatography, Gel; D002855:Chromatography, Thin Layer; D003600:Cytosol / Q000737:chemistry; D026261:Diazepam Binding Inhibitor; D004075:Diglycerides / Q000096:biosynthesis; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D005260:Female; D006243:Harderian Gland / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D050356:Lipid Metabolism; D008322:Mammals / Q000378:metabolism; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins; D009928:Organ Specificity; D016415:Sequence Alignment; D013045:Species Specificity; D025521:Tumor Suppressor Proteins
chemical_list: D002352:Carrier Proteins; D026261:Diazepam Binding Inhibitor; D004075:Diglycerides; C107608:FABP7 protein, human; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D009363:Neoplasm Proteins; D025521:Tumor Suppressor Proteins