Electron microscopy of buffalo green monkey kidney cells persistently infected with hepatitis A virus and immunolocalization of HAV antigens.
Studies were carried out to analyse the ultrastructural changes and the distribution of hepatitis A virus (HAV)/antigens at subcellular level in buffalo green monkey kidney (BGMK) cells persistently infected with HM-175 strain of HAV. HAV infected BGMK cells showed distinct abnormalities in the endoplasmic reticulum and cytoplasmic membrane as compared to uninfected cells. The abnormalities were characterized by wavy arrays, structures like myelin, annulate lamellae, cytoplasmic inclusion bodies and vesicles. The wavy arrays within the cytoplasm of the host cells appeared to represent degenerating membranes. A complex myelin like body was found in close association with a group of virus like particles. Annulate lamellae like structures involving single paired membrane were detected infrequently whereas the cytoplasmic vesicles were numerous in these cells. An indirect immunogold technique was utilized to localize the HAV antigenin infected cells. A high density immunogold label for HIV like particles was predominantly detected in cytoplasmic vesicles. These results suggest a strong association of membrane substructure in vesicle forms with the compartmentalized replication of HAV within persistently infected host cells.
Basu
A
A
National Institute of Virology, 20A, Dr. Ambedkar Road, Pune.
Gangodkar
S V
SV
Joshi
M S
MS
Chitambar
S D
SD
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D002460:Cell Line; D019084:Fluorescent Antibody Technique, Indirect / Q000379:methods; D006506:Hepatitis A / Q000821:virology*; D035921:Hepatitis A Antigens; D006507:Hepatovirus / Q000302:isolation & purification / Q000472:pathogenicity*; D007150:Immunohistochemistry / Q000379:methods; D007668:Kidney / Q000166:cytology* / Q000648:ultrastructure* / Q000821:virology; D008854:Microscopy, Electron
D000956:Antigens, Viral; D035921:Hepatitis A Antigens