[Laboratory diagnosis of toxoplasmosis using polymerase chain reaction].
Polymorphism of clinical manifestations in Toxoplasma infection and variegated disease patterns virtually rule out the diagnosis based solely on clinical symptoms, which makes modern laboratory tests particularly important. Amplification test system based on the polymerase chain reaction has been developed for the diagnosis of Toxoplasma infection. Computer analysis of nucleotide sequence of Toxoplasma gondii surface antigen gene P30 was analyzed, which helped choose and synthesize specific oligonucleotide primers. A method for biological material processing was selected, allowing sufficient DNA output. Optimal conditions for amplification reaction, ensuring absolute specificity and high (10-100 cells/sample) sensitivity, were determined.
Kuznetsova
E A
EA
Romanova
Iu M
IuM
Nesterenko
L N
LN
Benediktov
I I
II
D000818:Animals; D016054:DNA, Protozoan / Q000032:analysis*; D006801:Humans; D016133:Polymerase Chain Reaction; D014122:Toxoplasma / Q000302:isolation & purification*; D014123:Toxoplasmosis / Q000175:diagnosis*
D016054:DNA, Protozoan