Influence of Ukrain and cyclophosphamide administration on HA-1 murine hepatoma and LS lymphoma on aspartic proteinase cathepsin D.
Cathepsin D, the major lysosomal aspartyl proteinase and a mediator of interferon-gamma and tumor necrosis factor-alpha-induced apoptosis, was studied in murine models of LS lymphosarcoma treated by cyclophosphamide (possible apoptosis induction), and HA-1 hepatoma treated by Ukrain (positive antitumor effect). It was found that cyclophosphamide, as well as cyclophosphamide plus Ukrain, increased cathepsin D specific activity in mice with LS lymphosarcoma. Ukrain alone had no effect on cathepsin D activity in LS lymphosarcoma. In HA-1 hepatoma cells cathepsin D activity was not changed compared with intact normal murine liver (day 10) and activity decreased during tumor development (on day 12). Ukrain significantly increased cathepsin D activity in ascitic fluid (day 10) and had a tendency to increase cathepsin D activity in ascitic cells but not to the normal value.
Djanayeva
S J
SJ
Institute of Physiology, Siberian Branch, Russian Academy of Medical Sciences, Novosibirsk, Russia.
Korolenko
T A
TA
Svechnikova
I G
IG
Falameyeva
O V
OV
Korolenko
E
E
Kaledin
V I
VI
Nowicky
J
J
D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D018906:Antineoplastic Agents, Alkylating / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D001600:Berberine Alkaloids; D014408:Biomarkers, Tumor; D002402:Cathepsin D / Q000378:metabolism*; D003520:Cyclophosphamide / Q000494:pharmacology*; D004797:Enzyme-Linked Immunosorbent Assay; D008099:Liver / Q000473:pathology; D008114:Liver Neoplasms, Experimental / Q000378:metabolism* / Q000473:pathology; D008228:Lymphoma, Non-Hodgkin / Q000378:metabolism* / Q000473:pathology; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D010617:Phenanthridines
D000470:Alkaloids; D018906:Antineoplastic Agents, Alkylating; D000972:Antineoplastic Agents, Phytogenic; D001600:Berberine Alkaloids; D014408:Biomarkers, Tumor; D010617:Phenanthridines; C060921:ukrain; D003520:Cyclophosphamide; D002402:Cathepsin D