Production of 3-nitrocatechol by oxygenase-containing bacteria: optimization of the nitrobenzene biotransformation by Nocardia S3.
Twenty-one microorganisms were screened for their ability to convert nitroaromatics into 3-nitrocatechol as a result of the action of an oxygenase. Cultures containing toluene dioxygenases and phenol monooxygenases accumulated 3-nitrocatechol during incubation with nitrobenzene and nitrophenol, respectively. Nocardia S3 was selected and studied in more detail. Toluene-pregrown cultures were able to degrade nitrobenzene with a concomitant formation of 3-nitrocatechol. The rates of nitrobenzene utilization decreased throughout the biotransformation period and finally the accumulation ceased. The gradual deterioration of the biotransformation rates was not a consequence of depletion of the NADH pool, but was due to the accumulation of 3-nitrocatechol. The inhibition of nitrobenzene biotransformation by 3-nitrocatechol greatly impacts 3-nitrocatechol production processes.
Kieboom
J
J
Department of Food Technology and Nutritional Sciences, Wageningen Agricultural University, The Netherlands. Jasper.Kieboom@imb.ftns.wau.nl
van den Brink
H
H
Frankena
J
J
de Bont
J A
JA
D001711:Biotransformation; D002396:Catechols / Q000378:metabolism*; D003470:Culture Media; D009578:Nitrobenzenes / Q000378:metabolism*; D009596:Nitrophenols / Q000378:metabolism; D009615:Nocardia / Q000201:enzymology / Q000378:metabolism*; D010088:Oxidoreductases / Q000378:metabolism; D010101:Oxygen Consumption; D010105:Oxygenases / Q000378:metabolism*; D014050:Toluene / Q000378:metabolism
D002396:Catechols; D003470:Culture Media; D009578:Nitrobenzenes; D009596:Nitrophenols; D014050:Toluene; C036077:nitrobenzene; D010088:Oxidoreductases; D010105:Oxygenases; C023172:toluene dioxygenase; C034221:catechol