Anticancer drugs as inducers of thermotolerance in yeast.
Yeast cell viability was evaluated microscopically following exposure to heat shock for 30 min at 53 degrees C. The cells were previously grown in the presence of potential stressors (anticancer drugs; e.g., 5-fluorouracil, methotrexate, cisplatin, bleomycin, mitomycin-C and camptothecin-11). The induction of thermotolerance was documented by significantly increased viability after heat shock. This effect, which was reversed by cycloheximide, was comparable to that observed following exposure to a mild heat stress. These data demonstrate that pretreatment with sub-toxic concentrations of some of the clinically used antineoplastic agents conferres thermotolerance to yeast, possibly through the synthesis of protein components.
Miligkos
V
V
Department of Experimental Pharmacology, Medical School, University of Athens, 115 27 Athens, Greece.
Tiligada
E
E
Papamichael
K
K
Ypsilantis
E
E
Delitheos
A
A
D000970:Antineoplastic Agents / Q000494:pharmacology*; D001761:Bleomycin / Q000494:pharmacology; D002166:Camptothecin / Q000031:analogs & derivatives / Q000494:pharmacology; D002945:Cisplatin / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005472:Fluorouracil / Q000494:pharmacology; D006358:Hot Temperature; D000077146:Irinotecan; D008727:Methotrexate / Q000494:pharmacology; D016685:Mitomycin / Q000494:pharmacology; D012440:Saccharomyces / Q000187:drug effects* / Q000502:physiology
D000970:Antineoplastic Agents; D001761:Bleomycin; D016685:Mitomycin; D000077146:Irinotecan; D002945:Cisplatin; D005472:Fluorouracil; D002166:Camptothecin; D008727:Methotrexate