A quartz crystal resonant sensor (QCRS) study of HSA--drug interactions.
Human serum albumin (HSA) was immobilised on the gold surface of a quartz crystal resonance sensor (QCRS) and exposed to warfarin and diazepam. Distinct decreases in frequency of differing magnitudes were observed upon exposure of the protein to each of the compounds suggesting strongly that a ligand interaction was occurring. Moreover, as sequential exposure in any order was observed to yield distinct repeatable frequency decreases for the ligands indicated, screening for site specific binding may be possible. Identically immobilised bovine serum albumin (BSA) gave no response to either compound.
Pavey
K D
KD
School of Pharmacy and Biomolecular Sciences, University of Brighton, Lewes Road, Brighton, East Sussex, UK BN2 4GJ.
Lyle
E L
EL
Olliff
C J
CJ
Paul
F
F
D000818:Animals; D000925:Anticoagulants / Q000378:metabolism*; D001665:Binding Sites; D002417:Cattle; D003975:Diazepam / Q000378:metabolism*; D017022:Flow Injection Analysis; D006046:Gold; D006801:Humans; D006993:Hypnotics and Sedatives / Q000378:metabolism*; D009991:Oscillometry / Q000379:methods; D011485:Protein Binding; D011791:Quartz; D012709:Serum Albumin / Q000378:metabolism*; D012710:Serum Albumin, Bovine / Q000378:metabolism; D014859:Warfarin / Q000378:metabolism*
D000925:Anticoagulants; D006993:Hypnotics and Sedatives; D012709:Serum Albumin; D011791:Quartz; D012710:Serum Albumin, Bovine; D014859:Warfarin; D006046:Gold; D003975:Diazepam