title: A new in vivo model for testing cartilage grafts and biomaterials: the 'rabbit pinna punch-hole' model.
abstract: In this study an animal model was developed for evaluation of the feasibility of cartilage grafts. In the cartilage of the external ear of the rabbit multiple holes, 6 mm in diameter, were punched, leaving the adherent skin intact. Different experimental groups were evaluated. First, the punch-hole model was validated under various conditions to study spontaneous or perichondrial initiated regeneration of the cartilage defect. When both cartilage and perichondrium was excised no spontaneous repair of the cartilage defect was observed. When perichondrium is present, variable patch-like closure of the punch hole was found. As 'golden standard' a punched out piece of cartilage was reimplanted directly. This condition showed adequate closure of the punch hole, however, no perfect integration of graft and surrounding cartilage was observed. Secondly, to evaluate the 'punch-hole model' a biomaterial, trabecular demineralized bovine bone matrix (DBM), was implanted and tested as a scaffold for tissue engineering techniques in vivo and in vitro. Direct implantation of DBM did not lead to any cartilage formation to close the defect. In vivo engineered cartilage, generated by enveloping DBM in perichondrium for 3 weeks, could adequately close the punch hole. When DBM was seeded with isolated chondrocytes in vitro before implantation in the defect, a highly fragmented graft, with some islets of viable cells was seen. To promote an efficient and reliable evaluation of cartilage grafts a semi-quantitative grading system was developed. Items such as quality, quantity and integrity of the cartilage graft were included in a histomorphological grading system to provide information about the properties of a specific cartilage graft. To validate the grading system, all conditions were scored by two independent observers. An excellent reliability (R = 0.96) was seen between the observers. In summary, the rabbit pinna punch-hole model is a reliable and efficient method for first evaluation of cartilage grafts. The results can be easily analyzed using a semi-quantitative grading system.
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, Erasmus University Medical Center Rotterdam, The Netherlands.
authors.forename: P G
authors.initials: PG
authors.lastname: ten Koppel
authors.forename: G J
authors.initials: GJ
authors.lastname: van Osch
authors.forename: C D
authors.initials: CD
authors.lastname: Verwoerd
authors.forename: H L
authors.initials: HL
authors.lastname: Verwoerd-Verhoef
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D002356:Cartilage / Q000637:transplantation*; D008954:Models, Biological; D011817:Rabbits
chemical_list: D001672:Biocompatible Materials