Effects of intra-arterial administration of prostaglandin E1 on rat cremaster muscle microcirculation.
In plastic and reconstructive surgery, postoperative drug therapy with prostaglandin E1 (PGE1) has been widely used to prevent flap necrosis and to extend flap surviving area. In the present study, we assessed the effects of intra-arterial administration of PGE1 on microcirculation. The left cremaster muscle of male Wistar rats were used to measure microcirculatory hemodynamic parameters. The values of internal vessel diameter and erythrocyte velocity were measured by using the confocal laser-scanning microscope (CLSM) system and fluorescent dyes. The blood flow rate was calculated from measured internal vessel diameter and erythrocyte velocity. Internal vessel diameter and flowing erythrocytes were satisfactorily visualized by using the CLSM system. The blood flow rate of arteriole, venule and capillary were dose dependently increased by the intra-arterial administration of PGE1 from the minimum dose of 0.01 ng/kg/min. It is suggested that the intra-arterial administration of PGE1 is a more effective way of postoperative drug therapy than intravenous injection for flap surgery.
Natsui
H
H
Department of Biomedical Engineering, Institute of Basic Medical Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba Science City, Ibaraki, 305-8575, Japan.
Yanagi
K
K
Nakayama
Y
Y
Ohshima
N
N
D000527:Alprostadil / Q000494:pharmacology*; D000818:Animals; D001794:Blood Pressure; D007269:Injections, Intra-Arterial; D008297:Male; D008833:Microcirculation / Q000187:drug effects; D008853:Microscopy / Q000379:methods; D018482:Muscle, Skeletal / Q000098:blood supply* / Q000601:surgery*; D051381:Rats; D017208:Rats, Wistar; D013524:Surgical Flaps; D014665:Vasodilator Agents / Q000494:pharmacology*
D014665:Vasodilator Agents; D000527:Alprostadil