Discovery of a non-peptide small molecule that selectively mimics the biological actions of calcitonin.
Calcitonin (CT), a 32-amino acid peptide hormone secreted mainly from the thyroid gland, plays an important role in maintaining bone homeostasis. To discover non-peptide small molecules with biological actions similar to those of CT, a cell-based screening of an in-house chemical library was performed and a pyridone derivative (SUN B8155) was identified. Like CT, it elevated cyclic AMP (cAMP) levels in T47D and UMR106-06 cells which endogenously express human and rat CT receptor, respectively. SUN B8155 also stimulated cAMP formation in cells expressing recombinant human CT receptor, but not in those expressing human parathyroid hormone/parathyroid hormone-related peptide receptor. Accumulation of cAMP in T47D cells was blocked by a selective antagonist of CT receptor, salmon CT(8-32), whereas SUN B8155 did not displace the specific binding of [(125)I]CT to the receptor. Our results suggested that the compound selectively interacts with the CT receptor by a mechanism similar to but probably different from that of CT itself. In rats, intraperitoneal administration of SUN B8155 significantly lowered serum calcium levels, like CT. Our results demonstrate, for the first time, that the biological activities of the newly identified small molecule can mimic that of CT, acting via the CT receptor.
Katayama
T
T
Suntory Biomedical Research Limited, 1-1-1 Wakayamadai, Shimamoto-cho, Mishima-gun, 618-8503, Osaka, Japan.
Furuya
M
M
Yamaichi
K
K
Konishi
K
K
Sugiura
N
N
Murafuji
H
H
Magota
K
K
Saito
M
M
Tanaka
S
S
Oikawa
S
S
D000818:Animals; D001667:Binding, Competitive; D002116:Calcitonin / Q000235:genetics / Q000494:pharmacology / Q000502:physiology*; D002118:Calcium / Q000097:blood; D002460:Cell Line; D000242:Cyclic AMP / Q000378:metabolism; D005822:Genetic Vectors; D006801:Humans; D008956:Models, Chemical; D015394:Molecular Structure; D011725:Pyridines / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D051381:Rats; D018003:Receptors, Calcitonin / Q000819:agonists* / Q000096:biosynthesis / Q000235:genetics; D014162:Transfection
D011725:Pyridines; D018003:Receptors, Calcitonin; C427644:SUN B8155; D002116:Calcitonin; D000242:Cyclic AMP; D002118:Calcium