title: Flow cytometric analysis of the localization of Helicobacter pylori antigens during different growth phases.
abstract: Previous studies on the localization of several different Helicobacter pylori antigens have been contradictory. We have therefore examined by using both one- and two-color flow cytometry (FCM), immunofluorescence (IF), and immunoelectron microscopy (IEM), the possible surface localization of some H. pylori antigens that may be important virulence factors. All four methods detected the lipopolysaccharide and the N-acetyl-neuroaminyllactose-binding hemagglutinin protein (HpaA) as surface-exposed, while the urease enzyme was not detected at all and the neutrophil activating protein only in low concentration on the surface of the H. pylori bacteria during culture of H. pylori in liquid broth for 11 days. The FCM analysis was found to be quite sensitive and specific and also extremely fast compared with IF and IEM, and therefore the preferred method for detection of surface-localized antigens of H. pylori.
authors.affiliation: Department of Medical Microbiology and Immunology, Göteborg University, P.O. Box 435, SE-405 30, Göteborg, Sweden.
authors.forename: K
authors.initials: K
authors.lastname: Blom
authors.forename: B S
authors.initials: BS
authors.lastname: Lundin
authors.forename: I
authors.initials: I
authors.lastname: Bölin
authors.forename: A
authors.initials: A
authors.lastname: Svennerholm
mesh_terms: D018829:Adhesins, Bacterial; D000942:Antigens, Bacterial / Q000032:analysis*; D000954:Antigens, Surface / Q000032:analysis; D001426:Bacterial Proteins; D003470:Culture Media; D005434:Flow Cytometry / Q000379:methods*; D005455:Fluorescent Antibody Technique; D016480:Helicobacter pylori / Q000201:enzymology / Q000254:growth & development / Q000276:immunology*; D006388:Hemagglutinins / Q000032:analysis; D037102:Lectins; D008070:Lipopolysaccharides / Q000032:analysis; D008074:Lipoproteins; D016253:Microscopy, Immunoelectron; D012680:Sensitivity and Specificity; D014510:Urease / Q000032:analysis
chemical_list: D018829:Adhesins, Bacterial; D000942:Antigens, Bacterial; D000954:Antigens, Surface; D001426:Bacterial Proteins; D003470:Culture Media; D006388:Hemagglutinins; C096437:HpaA protein, bacteria; D037102:Lectins; D008070:Lipopolysaccharides; D008074:Lipoproteins; C092412:neutrophil-activating protein A, Helicobacter pylori; C095977:adhesin A, Helicobacter; D014510:Urease