title: Measurement of fecal sulfide using gas chromatography and a sulfur chemiluminescence detector.
abstract: We describe a simple technique to measure sulfide in fecal homogenates (or any other liquid milieu), which involves acidification followed by the G.C. measurement of H2S in a gas space equilibrated with a small quantity of homogenate. An internal standard of Zn35S added to the homogenate permits correction for incomplete recovery of H2S in the gas space. The use of a sulfur chemiluminescence detector, which specifically and sensitively responds to sulfur-containing compounds, greatly facilitates this measurement.
authors.affiliation: Department of Veterans Affairs, Medical Centre, Minneapolis, MN 55417, USA.
authors.forename: J K
authors.initials: JK
authors.lastname: Fume
authors.forename: J
authors.initials: J
authors.lastname: Springfield
authors.forename: T
authors.initials: T
authors.lastname: Koenig
authors.forename: F
authors.initials: F
authors.lastname: Suarez
authors.forename: M D
authors.initials: MD
authors.lastname: Levitt
mesh_terms: D002849:Chromatography, Gas / Q000379:methods*; D005243:Feces / Q000737:chemistry*; D006801:Humans; D006862:Hydrogen Sulfide / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D008163:Luminescent Measurements; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013440:Sulfides; D013455:Sulfur / Q000032:analysis; D017967:Zinc Compounds
chemical_list: D013440:Sulfides; D017967:Zinc Compounds; D013455:Sulfur; C031238:zinc sulfide; D006862:Hydrogen Sulfide