The histochemical profiles of fibre types in porcine skeletal muscle.
Using a variety of histochemical methods -mATPase staining after alkaline and acid preincubations, NADH-TR and alpha-MGPDH- we have investigated the fibre types in porcine skeletal muscle. The results reveal that four major fibre types -I, IIA, IIB and II*- can be separated histochemically in Longissimus lumborum muscle of Landrace pigs. The histochemical properties of the muscle fibre type II* are very similar to that of type IIX described in other mammals. The existence of IIX fibres in pig muscle has been recently demonstrated by molecular biology techniques and our results validate the use of histochemistry (mATPase) as an easy methodology to differentiate the three fast myosins (type II fibres) in pig muscle.
Gil
F
F
Department of Anatomy and Embryology, Faculty of Veterinary, University of Murcia, Spain. cano@um.es
López-Albors
O
O
Vázquez
J M
JM
Latorre
R
R
Ramírez-Zarzosa
G
G
Moreno
F
F
D000251:Adenosine Triphosphatases / Q000032:analysis; D000818:Animals; D005993:Glycerolphosphate Dehydrogenase / Q000032:analysis; D006651:Histocytochemistry / Q000379:methods; D018485:Muscle Fibers, Skeletal / Q000737:chemistry* / Q000201:enzymology; D018482:Muscle, Skeletal / Q000033:anatomy & histology* / Q000201:enzymology; D009246:NADH Tetrazolium Reductase / Q000032:analysis; D013552:Swine / Q000033:anatomy & histology*
D005993:Glycerolphosphate Dehydrogenase; D009246:NADH Tetrazolium Reductase; D000251:Adenosine Triphosphatases