title: Functional and phenotypic characterization of distinct porcine dendritic cells derived from peripheral blood monocytes.
abstract: Dendritic cells (DCs) are bone marrow-derived antigen-presenting cells that have an exquisite capacity to interact with T cells and modulate their responses. Little is known about porcine DCs despite the fact that they represent an important target in strategies that are aimed at modulating resistance to infection in pigs and may be of major importance in transplantation biology. We generated immature monocyte-derived porcine dendritic cells (MoDCs) directly from adherent peripheral blood cells treated with porcine granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4). The cells were observed via electron microscopy and their phenotype was characterized using monoclonal antibodies. The functionality of the porcine MoDCs was demonstrated showing that the cells were capable of different specialized functions relevant to antigen capture and were potent stimulators in a primary allo-mixed leucocyte reaction. Treatment of the MoDCs with porcine cell line-derived necrotic factors resulted in the phenotypic and functional maturation of MoDCs. We confirmed also that monocyte-derived DCs were differentially regulated by cytokines, showing that transforming growth factor-beta1 (TGF-beta1) is able to redirect monocytic precursors into the differentiation pathway of Langerhans' cells presenting typical Birbeck granules. Interestingly, and in contrast to the human and murine model, we showed that the monocyte-derived porcine Langerhans'-type cells (MoLCs) were much more potent activators of allogeneic T cells than MoDCs obtained without TGF-beta1.
authors.affiliation: Discovery Research, Merial, 254 rue Marcel Mérieux BP 7009, 69 342 Cedex 07, Lyon France.
authors.forename: R
authors.initials: R
authors.lastname: Paillot
authors.forename: F
authors.initials: F
authors.lastname: Laval
authors.forename: J C
authors.initials: JC
authors.lastname: Audonnet
authors.forename: C
authors.initials: C
authors.lastname: Andreoni
authors.forename: V
authors.initials: V
authors.lastname: Juillard
mesh_terms: D000818:Animals; D017951:Antigen Presentation / Q000276:immunology; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology* / Q000648:ultrastructure; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000276:immunology; D016130:Immunophenotyping; D015847:Interleukin-4 / Q000276:immunology; D007801:Langerhans Cells / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology; D007959:Lymphocyte Culture Test, Mixed; D008297:Male; D009000:Monocytes / Q000276:immunology*; D013552:Swine / Q000276:immunology*; D016212:Transforming Growth Factor beta / Q000276:immunology; D053773:Transforming Growth Factor beta1
chemical_list: C508960:TGFB1 protein, human; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; D015847:Interleukin-4; D016178:Granulocyte-Macrophage Colony-Stimulating Factor