Identification of a murine cysteinyl leukotriene receptor by expression in Xenopus laevis oocytes.
We report the identification of an EST encoding a murine cysteinyl leukotriene (mCysLT) receptor. LTD4, LTC4 and LTE4 but not LTB4 or various nucleotides activated Ca2+-evoked Cl- currents in mCysLT1 expressing Xenopus laevis oocytes. The response to LTD4 was blocked by MK-571, reduced by pretreatment with pertussis toxin (PTX), and was partly dependent on extracellular Ca2+. The identified murine CysLT1 receptor differs from the hCysLT1 receptor with regard to PTX sensitivity, receptor-mediated Ca2+ influx, and antagonist sensitivity.
Mollerup
J
J
Institut of Molecular Biology, Department of Molecular Cell Biology, Copenhagen, Denmark.
Jørgensen
S T
ST
Hougaard
C
C
Hoffmann
E K
EK
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites / Q000235:genetics; D002118:Calcium / Q000378:metabolism / Q000494:pharmacology; D002712:Chlorides / Q000378:metabolism; D020224:Expressed Sequence Tags; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D020024:Leukotriene Antagonists; D015289:Leukotrienes / Q000494:pharmacology; D008565:Membrane Proteins; D051379:Mice; D008969:Molecular Sequence Data; D009865:Oocytes / Q000187:drug effects / Q000378:metabolism; D037342:Pertussis Toxin; D011422:Propionates / Q000494:pharmacology; D011804:Quinolines / Q000494:pharmacology; D018077:Receptors, Leukotriene / Q000235:genetics* / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D010566:Virulence Factors, Bordetella / Q000494:pharmacology; D014982:Xenopus laevis
D002712:Chlorides; D020024:Leukotriene Antagonists; D015289:Leukotrienes; D008565:Membrane Proteins; D011422:Propionates; D011804:Quinolines; D018077:Receptors, Leukotriene; D010566:Virulence Factors, Bordetella; C059141:verlukast; C412375:cysteinyl leukotriene receptor 2; D037342:Pertussis Toxin; C083169:leukotriene D4 receptor; D002118:Calcium