Phosphate stimulates differentiation and mineralization of the chondroprogenitor clone ATDC5.
ATDC5 cells were employed to examine how inorganic phosphate (Pi) influences chondrocytic bone formation. 1) Pi (3 - 30 mM) plus ascorbic acid (50 microg/ml) dose-dependently accelerated proliferative differentiation and mineralization of ATDC5. 2) Northern blot analysis revealed that 10 mM Pi suppressed expression of type II collagen and PTH (parathyroid hormone) / PTH-related peptide (PTHrP) receptor, while it accelerated type X collagen expression. 3) Pi (3 - 30 mM) dose-dependently increased luciferase activity in the cells transfected with 3000 bp type X collagen promoter fused to the luciferase gene. The results suggest a regulatory role of Pi in endochondral osteogenesis.
Fujita
T
T
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Setsunan University, Hirakata, Japan.
Meguro
T
T
Izumo
N
N
Yasutomi
C
C
Fukuyama
R
R
Nakamuta
H
H
Koida
M
M
D000818:Animals; D015415:Biomarkers / Q000032:analysis; D015152:Blotting, Northern; D002113:Calcification, Physiologic / Q000502:physiology*; D002454:Cell Differentiation; D019902:Chondrocytes / Q000166:cytology* / Q000378:metabolism; D002999:Clone Cells; D003094:Collagen / Q000378:metabolism; D051379:Mice; D010281:Parathyroid Hormone / Q000378:metabolism; D044162:Parathyroid Hormone-Related Protein; D010710:Phosphates / Q000378:metabolism* / Q000494:pharmacology; D011506:Proteins / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism
D015415:Biomarkers; D010281:Parathyroid Hormone; D044162:Parathyroid Hormone-Related Protein; D010710:Phosphates; D011506:Proteins; D003094:Collagen