Platelet activating factor degradation in tear fluid from guinea pigs with allergic conjunctivitis.
The purpose of this study was to investigate the role of platelet-activating factor (PAF) and PAF acetylhydrolase (AH) in conjunctiva. The influence of PAF on conjunctival vascular permeability and the presence of PAF or its metabolites in tears from guinea pigs with allergic conjunctivitis were investigated. We instilled PAF to the eyes of guinea pigs and evaluated vascular permeability. Tear samples were collected from passively sensitized guinea pigs, and the concentration of PAF and its metabolites determined by liquid chromatography-tandem mass spectrometry. Exogenous PAF degradation in tear samples was evaluated with or without diisopropyl fluorophosphate (DFP). Topically applied PAF increased vascular permeability in conjunctiva. In the tear samples from guinea pigs with allergic conjunctivitis, PAF could not be detected. However, 40 +/- 6 ng/ml of lyso-platelet activating factor (lyso-PAF) and 230 +/- 50 ng/ml of 1-alkyl-2-acyl-sn-glycero-3-phosphocholine were detected at 10 min after challenge. Exogenous PAF was rapidly degraded in the tear samples from guinea pigs with allergic conjunctivitis, but not from normal guinea pigs. This PAF degradation was inhibited by DFP. These results suggest that PAF in the tear fluid is quickly hydrolyzed to lyso-PAF by PAF AH, which may be released or activated in allergic conjunctivitis.
Kato
M
M
Ophthalmic Research Division, Santen Pharmaceutical Co., Lt., Nara, Japan.
Mano
H
H
Ota
A
A
Konomi
K
K
Nakata
K
K
D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase; D000818:Animals; D002199:Capillary Permeability / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D003228:Conjunctiva / Q000098:blood supply; D003233:Conjunctivitis, Allergic / Q000378:metabolism*; D006168:Guinea Pigs; D007531:Isoflurophate / Q000494:pharmacology; D008297:Male; D010741:Phospholipases A / Q000378:metabolism; D010972:Platelet Activating Factor / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000378:metabolism*; D013666:Tears / Q000378:metabolism*
C036761:1-alkyl-2-acyl-sn-glycero-3-phosphocholine; C029271:O-deacetyl platelet activating factor; D010972:Platelet Activating Factor; D007531:Isoflurophate; D010741:Phospholipases A; D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase