The effect of mild hypothermia and induced hypertension on long term survival rate and neurological outcome after asphyxial cardiac arrest in rats.
OBJECTIVE
we studied the long-term effect of a combined treatment with resuscitative mild hypothermia and induced hypertension on survival rate and neurological outcome after asphyxial cardiac arrest (CA) in rats.


METHODS
36 male Wistar rats, were randomised into three groups: Group I (n=10): anaesthetised with halothane and N(2)O/O(2) (70/30%) had vessel cannulation but no asphyxial CA; mechanical ventilation was continued to 1 h. Group II (n=13): under the same anaesthetic conditions and vessel cannulation, was subjected to asphyxial CA of 8 min, reversed by brief external heart massage and followed by mechanical ventilation to 1 h post restoration of spontaneous circulation (ROSC). Group III (n=13): received the same insult and resuscitation as described in group II, but in contrast to the previous group, a combination treatment of hypothermia (34 degrees C) and induced hypertension was started immediately after ROSC and maintained for 60 min ROSC. Survival rate and neurological deficit (ND) scores were determined before arrest, at 2 and 24 h, and each 24-h up to 4 weeks after ROSC.


RESULTS
Baseline variables were the same in the three groups. Comparison of the asphyxial CA groups (groups II and III), showed an increased, although not statistically significant, survival rate at 72 h after ROSC in group III, and it became highly significant at 4 weeks after ROSC. The ND scores were the same in both asphyxial CA groups (groups II and III).


CONCLUSIONS
Resuscitative mild hypothermia and induced hypertension after asphyxial CA in rats is associated with a better survival rate. This beneficial effect persisted for 4 weeks after ROSC.
Hachimi-Idrissi
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S
Department of Critical Care Medicine and Cerebral Resuscitation Research Group, Vrije Universiteit Brussel, Laarbeeklaan, 101, B-1090 Brussels, Belgium. ndphiis@az.vub.ac.be
Corne
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Huyghens
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D000818:Animals; D001237:Asphyxia / Q000401:mortality / Q000503:physiopathology; D006323:Heart Arrest / Q000401:mortality* / Q000503:physiopathology / Q000628:therapy; D006973:Hypertension / Q000503:physiopathology*; D007036:Hypothermia, Induced; D008297:Male; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012151:Resuscitation; D015996:Survival Rate; D013997:Time Factors