title: Chloride channels regulate HIT cell volume but cannot fully account for swelling-induced insulin secretion.
abstract: Insulin-secreting pancreatic islet beta-cells possess anion-permeable Cl- channels (I(Cl,islet)) that are swelling-activated, but the role of these channels in the cells is unclear. The Cl- channel blockers 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS) and niflumic acid were evaluated for their ability to inhibit I(Cl,islet) in clonal beta-cells (HIT cells). Both drugs blocked the channel, but the blockade due to niflumic acid was less voltage-dependent than the blockade due to DIDS. HIT cell volume initially increased in hypotonic solution and was followed by a regulatory volume decrease (RVD). The addition of niflumic acid and, to a lesser extent, DIDS to the hypotonic solution potentiated swelling and blocked the RVD. In isotonic solution, niflumic acid produced swelling, suggesting that islet Cl- channels are activated under basal conditions. The channel blockers glyburide, gadolinium, or tetraethylammonium-Cl did not alter hypotonic-induced swelling or volume regulation. The Na/K/2Cl transport blocker furosemide produced cell shrinkage in isotonic solution and blocked cell swelling normally induced by hypotonic solution. Perifused HIT cells secreted insulin when challenged with hypotonic solutions. However, this could not be completely attributed to I(Cl,islet)-mediated depolarization, because secretion persisted even when Cl- channels were fully blocked. To test whether blocker-resistant secretion occurred via a distal pathway, distal secretion was isolated using 50 mmol/l potassium and diazoxide. Under these conditions, glucose-dependent secretion was blunted, but hypotonically induced secretion persisted, even with Cl- channel blockers present. These results suggest that beta-cell swelling stimulates insulin secretion primarily via a distal I(Cl,islet)-independent mechanism, as has been proposed for K(ATP)-independent glucose- and sulfonylurea-stimulated insulin secretion. Reverse transcriptase-polymerase chain reaction of HIT cell mRNA identified a CLC-3 transcript in HIT cells. In other systems, CLC-3 is believed to mediate swelling-induced outwardly rectifying Cl- channels. This suggests that the proximal effects of swelling to regulate cell volume may be mediated by CLC-3 or a closely related Cl- channel.
authors.affiliation: Department of Pharmacology and Toxicology, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298-0524, USA.
authors.forename: T A
authors.initials: TA
authors.lastname: Kinard
authors.forename: P B
authors.initials: PB
authors.lastname: Goforth
authors.forename: Q
authors.initials: Q
authors.lastname: Tao
authors.forename: M E
authors.initials: ME
authors.lastname: Abood
authors.forename: J
authors.initials: J
authors.lastname: Teague
authors.forename: L S
authors.initials: LS
authors.lastname: Satin
mesh_terms: D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid / Q000494:pharmacology*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism; D048429:Cell Size / Q000502:physiology*; D018118:Chloride Channels / Q000037:antagonists & inhibitors / Q000235:genetics / Q000502:physiology*; D002999:Clone Cells; D006224:Cricetinae; D003981:Diazoxide / Q000494:pharmacology; D005665:Furosemide / Q000494:pharmacology; D005682:Gadolinium / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology; D005905:Glyburide / Q000494:pharmacology; D006706:Homeostasis; D007038:Hypotonic Solutions; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D007700:Kinetics; D008969:Molecular Sequence Data; D009544:Niflumic Acid / Q000494:pharmacology*; D028021:Sodium-Potassium-Chloride Symporters; D019789:Tetraethylammonium / Q000494:pharmacology
chemical_list: D002352:Carrier Proteins; D018118:Chloride Channels; D007038:Hypotonic Solutions; D007328:Insulin; D028021:Sodium-Potassium-Chloride Symporters; D009544:Niflumic Acid; D019789:Tetraethylammonium; D005665:Furosemide; D005682:Gadolinium; D005947:Glucose; D003981:Diazoxide; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; D005905:Glyburide