Development and assignment of bovine-specific PCR systems for the Texas nomenclature marker genes and isolation of homologous BAC probes.
In 1996, Popescu et al. published the Texas standard nomenclature of the bovine karyotype in which 31 marker genes, already mapped in man, were chosen to permit unambiguous identification and numbering of each bovine chromosome. However, specific PCR systems were not available for each marker gene thus preventing the assignment of part of these markers by somatic cell hybrid analysis. In addition, some difficulties remained with the nomenclature of BTA25, BTA27 and BTA29. In this work, specific PCR systems were developed for each of the marker genes except VIL1 (see results), from either existing bovine or human sequences, and a bovine BAC library was screened to obtain the corresponding BAC clones. These PCR systems were used successfully to confirm the assignment of each marker gene (except for LDHA, see results) by analysis on the INRA hamster-bovine somatic cell hybrid panel. The difficulties observed for LDHA and VIL1 are probably due to the fact that these genes belong to large gene families and therefore suggest that they may not be the most appropriate markers for a standardisation effort. This panel of BACs is available to the scientific community and has served as a basis for the establishment of a revised standard nomenclature of bovine chromosomes.
Gautier
M
M
Laboratoire de génétique biochimique et de cytogénétique, Département de génétique animale, Institut national de la recherche agronomique, 78352 Jouy-en-Josas Cedex, France.
Laurent
P
P
Hayes
H
H
Eggen
A
A
D000818:Animals; D002417:Cattle / Q000235:genetics*; D002871:Chromosome Banding / Q000592:standards; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics; D006224:Cricetinae; D017931:DNA Primers; D015342:DNA Probes; D019992:Databases as Topic / Q000145:classification; D005819:Genetic Markers; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D020161:Physical Chromosome Mapping / Q000592:standards; D016133:Polymerase Chain Reaction / Q000379:methods; D009626:Terminology as Topic; D013781:Texas
D017931:DNA Primers; D015342:DNA Probes; D005819:Genetic Markers