title: Mutations in the csgD promoter associated with variations in curli expression in certain strains of Escherichia coli O157:H7.
abstract: Single-base-pair csgD promoter mutations in human outbreak Escherichia coli O157:H7 strains ATCC 43894 and ATCC 43895 coincided with differential Congo red dye binding from curli fiber expression. Red phenotype csgD::lacZ promoter fusions had fourfold-greater expression than white promoter fusions. Cloning the red variant csgDEFG operon into white variants induced the red phenotype. Substrate utilization differed between red and white variants.
authors.affiliation: Roman L. Hruska U.S. Meat Animal Research Center, Agricultural Research Service, U.S. Department of Agriculture, Clay Center, Nebraska 68933, USA. guhlich@arserrc.gov
authors.forename: G A
authors.initials: GA
authors.lastname: Uhlich
authors.forename: J E
authors.initials: JE
authors.lastname: Keen
authors.forename: R O
authors.initials: RO
authors.lastname: Elder
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D001483:Base Sequence; D004927:Escherichia coli Infections / Q000382:microbiology; D019453:Escherichia coli O157 / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D029968:Escherichia coli Proteins; D014644:Genetic Variation; D006801:Humans; D009154:Mutation; D011401:Promoter Regions, Genetic / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D001426:Bacterial Proteins; C109182:CsgB protein, E coli; D029968:Escherichia coli Proteins; C489688:csgA protein, E coli; C077481:Crl protein, Bacteria