title: Real-time PCR assay targeting IS481 of Bordetella pertussis and molecular basis for detecting Bordetella holmesii.
abstract: Detection of Bordetella holmesii by a real-time PCR assay targeting IS481 of Bordetella pertussis is reported. Sequencing of IS481-specific PCR products from B. pertussis and B. holmesii isolates revealed sequence homology. Restriction fragment length polymorphism demonstrated a low copy number of IS481-like sequences in B. holmesii. These results, and culture of B. holmesii from patients with cough, suggest that the specificity and predictive value of IS481-based PCR assays for pertussis may be compromised.
authors.affiliation: Institute of Medical Microbiology and Hygiene, University of Regensburg, Franz-Josef-Strauss-Allee 11, D-93053 Regensburg, Germany. Udo.Reischl@klinik.uni-regensburg.de
authors.forename: U
authors.initials: U
authors.lastname: Reischl
authors.forename: N
authors.initials: N
authors.lastname: Lehn
authors.forename: G N
authors.initials: GN
authors.lastname: Sanden
authors.forename: M J
authors.initials: MJ
authors.lastname: Loeffelholz
mesh_terms: D001884:Bordetella / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001885:Bordetella Infections / Q000175:diagnosis / Q000382:microbiology*; D001886:Bordetella pertussis / Q000235:genetics*; D004251:DNA Transposable Elements / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D012680:Sensitivity and Specificity
chemical_list: D004251:DNA Transposable Elements