Occurrence of an unusual phosphorylated N-acetyllactosamine in horse colostrum.
The colostrum of horses (thoroughbreds) was extracted and fractionated to yield Gal(beta1-4)GlcNAcalpha1-phosphate, which has not previously been detected in any mammalian milk or colostrum, as well as Neu5Ac(alpha2-3)Gal(beta1-4)Glc. The structures of these saccharides were established by NMR spectroscopy and MALDI-TOF mass spectrometry.
Nakamura
T
T
Department of Bioresource, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan. nakamura@obihiro.ac.jp
Amikawa
S
S
Harada
T
T
Saito
T
T
Arai
I
I
Urashima
T
T
D000606:Amino Sugars / Q000737:chemistry* / Q000302:isolation & purification*; D000818:Animals; D002240:Carbohydrate Sequence; D003126:Colostrum / Q000737:chemistry*; D005260:Female; D006736:Horses; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry / Q000302:isolation & purification; D010766:Phosphorylation; D011247:Pregnancy; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
C421467:3'-sialyllactose; D000606:Amino Sugars; D009844:Oligosaccharides; C000458:N-acetyllactosamine