Osteoclastic resorption of biomimetic calcium phosphate coatings in vitro.
A new biomimetic method for coating metal implants enables the fast formation of dense and homogeneous calcium phosphate coatings. Titanium alloy (Ti6Al4V) disks were coated with a thin, carbonated, amorphous calcium phosphate (ACP) by immersion in a saturated solution of calcium, phosphate, magnesium, and carbonate. The ACP-coated disks then were processed further by incubation in calcium phosphate solutions to produce either crystalline carbonated apatite (CA) or octacalcium phosphate (OCP). The resorption behavior of these three biomimetic coatings was studied using osteoclast-enriched mouse bone-marrow cell cultures for 7 days. Cell-mediated degradation was observed for both carbonated apatite and octacalcium phosphate coatings. Numerous resorption lacunae characteristic of osteoclastic resorption were found on carbonated apatite after cell culture. The results showed that carbonated apatite coatings are resorbed by osteoclasts in a manner consistent with normal osteoclastic resorption. Osteoclasts also degraded the octacalcium phosphate coatings but not by classical pit formation.
Leeuwenburgh
S
S
Laboratory for Inorganic Chemistry, Delft University of Technology, Delft, The Netherlands.
Layrolle
P
P
Barrère
F
F
de Bruijn
J
J
Schoonman
J
J
van Blitterswijk
C A
CA
de Groot
K
K
D000497:Alloys; D000818:Animals; D001031:Apatites / Q000737:chemistry / Q000494:pharmacology*; D001672:Biocompatible Materials; D001854:Bone Marrow Cells / Q000166:cytology; D018786:Bone Substitutes; D002117:Calcitriol / Q000494:pharmacology; D002130:Calcium Phosphates / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology*; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible; D003460:Crystallization; D051379:Mice; D008819:Mice, Nude; D008855:Microscopy, Electron, Scanning; D010010:Osteoclasts / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D019736:Prostheses and Implants; D012996:Solutions; D013499:Surface Properties; D013997:Time Factors; D014025:Titanium / Q000737:chemistry
D000497:Alloys; D001031:Apatites; D001672:Biocompatible Materials; D018786:Bone Substitutes; D002130:Calcium Phosphates; D020099:Coated Materials, Biocompatible; D012996:Solutions; C519480:amorphous calcium phosphate; C031462:titanium alloy (TiAl6V4); D014025:Titanium; D002117:Calcitriol