A phosphodiesterase from ascites carcinoma Krebs II cells specifically cleaves the bond between VPg and RNA of encephalomyocarditis virus.
The substrate specificity of the "unlinking" enzyme from ascite carcinoma Krebs II cells has been investigated. The enzyme specifically splits the interpolymeric phosphodiester bond between Kp and the 5;-terminal phosphate group of the uridylic acid residue in the Kp-pUpUpGp complex.
Gulevich
A Y
AY
Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, 119899, Russia.
Yusupova
R A
RA
Drygin
Y F
YF
D000818:Animals; D001201:Ascites / Q000201:enzymology*; D002277:Carcinoma / Q000201:enzymology*; D002855:Chromatography, Thin Layer; D004686:Encephalomyocarditis virus / Q000378:metabolism*; D006868:Hydrolysis; D051379:Mice; D010727:Phosphoric Diester Hydrolases / Q000737:chemistry*; D011485:Protein Binding; D012313:RNA / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D014758:Viral Core Proteins / Q000378:metabolism*
D014758:Viral Core Proteins; D012313:RNA; D010727:Phosphoric Diester Hydrolases