Production of beta-carotene by a Rhodotorula glutinis mutant in sea water medium.
Mutant 32, derived from Rhodotorula glutinis NCIM 3353 produced 76-fold more beta-carotene than the parent strain. In the growth medium prepared in seawater, the total carotenoid content and dry cell mass was 86 mg/l and 16 g/l, respectively, as compared to 70 mg/l and 12 g/l obtained with a medium prepared in distilled water. A 2-fold increase in beta-carotene with simultaneous 2.3-fold decrease in torulene content was also observed. When grown in seawater medium at pH 6.0, 83 +/- 5% carotenoids could be extracted from the cells without any mechanical disintegration.
Bhosale
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Chemical Engineering Division, National Chemical Laboratory, Pune, India.
Gadre
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RV
D000362:Agar / Q000737:chemistry; D001487:Basidiomycota / Q000235:genetics* / Q000378:metabolism*; D002455:Cell Division; D002851:Chromatography, High Pressure Liquid; D006863:Hydrogen-Ion Concentration; D009154:Mutation; D012623:Seawater; D013997:Time Factors; D019207:beta Carotene / Q000096:biosynthesis*
D019207:beta Carotene; D000362:Agar