Genotypes of clustered cases of Pneumocystis carinii pneumonia.
Reports of outbreaks of Pneumocystis carinii pneumonia (PCP) among human immunodeficiency virus-negative immunocompromised patients have suggested a person-to-person transmission of P. carinii. In this study, 17 bronchoalveolar lavage isolates from patients in 3 PCP outbreaks were genotyped, 2 in renal transplant recipients and 1 outbreak among patients with haematological disorders. Genotypes in the P. carinii sp. f. hominis (P. carinii f.sp. hominis) mt large subunit ribosomal RNA site 85 were detected by 2 methods: direct sequencing and 3 different allele-specific polymerase chain reaction assays. Although limited data on patient contacts were available, the detected P. c. hominis genotypes do not support person-to-person transmission as the predominant transmission route of P. carinii in humans.
Olsson
M
M
Swedish Institute for Infectious Disease Control and Microbiology and Tumor Biology Center, Karolinska Institutet, Stockholm, Sweden.
Eriksson
B M
BM
Elvin
K
K
Strandberg
M
M
Wahlgren
M
M
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001992:Bronchoalveolar Lavage Fluid / Q000382:microbiology; D004196:Disease Outbreaks; D018562:Disease Transmission, Infectious; D005260:Female; D005838:Genotype; D006402:Hematologic Diseases / Q000150:complications / Q000276:immunology; D006761:Hospitals; D006801:Humans; D016867:Immunocompromised Host; D016030:Kidney Transplantation / Q000009:adverse effects / Q000276:immunology; D008297:Male; D008875:Middle Aged; D011010:Pneumocystis / Q000235:genetics*; D011020:Pneumonia, Pneumocystis / Q000453:epidemiology / Q000382:microbiology* / Q000635:transmission; D012331:RNA, Fungal / Q000032:analysis; D012335:RNA, Ribosomal / Q000032:analysis; D013548:Sweden / Q000453:epidemiology
D012331:RNA, Fungal; D012335:RNA, Ribosomal