[Localization and expression of TR3 orphan receptor in mouse testis].
By means of in situ hybridization and immunohistochemistry, the expression and cellular localization of orphan receptor TR3 and its mRNA were observed. The results showed that both TR3 and its mRNA were expressed in a significant amount in mouse testis, and the expression level of TR3 mRNA was different in different seminiferous tubules. TR3 mRNA was specifically expressed in germ cells, mainly in spermatogonia and less advanced primary spermatocytes, whereas TR3 receptor protein was mainly localized in germ cells. It is suggested that TR3 may play an important role in regulating the early stage of germ cell development in mice.
Mu
X M
XM
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080.
Liu
Y X
YX
D000818:Animals; D004268:DNA-Binding Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D005854:Germ Cells / Q000737:chemistry / Q000378:metabolism; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D051379:Mice; D057105:Nuclear Receptor Subfamily 4, Group A, Member 1; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D018160:Receptors, Cytoplasmic and Nuclear; D011987:Receptors, Steroid; D011988:Receptors, Thyroid Hormone; D013091:Spermatogenesis; D013737:Testis / Q000737:chemistry* / Q000378:metabolism; D014157:Transcription Factors / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics
D004268:DNA-Binding Proteins; C539818:Nr4a1 protein, mouse; D057105:Nuclear Receptor Subfamily 4, Group A, Member 1; D012333:RNA, Messenger; D018160:Receptors, Cytoplasmic and Nuclear; D011987:Receptors, Steroid; D011988:Receptors, Thyroid Hormone; D014157:Transcription Factors