Effect of six flavonoids on proliferation of hepatic stellate cells in vitro.
OBJECTIVE
To study the effects of six flavonoids (fisetin, quercetin, apigenin, phloretin, hesperetin, and chalcone) on proliferation of hepatic stellate cell (HSC-T6 cells).


METHODS
Cell proliferation was measured by crystal violet staining assay.


RESULTS
Fisetin, quercetin, apigenin, phloretin, hesperetin, chalcone (6.25-50 mumol.L-1) inhibited the proliferation of HSC-T6 cells stimulated by serum, macrophage conditioned medium (MCM) and platelet-derived growth factor (PDGF) in a concentration-dependent manner. In the MCM-stimulated proliferation experiment, their IC50 were 21.48, 18.52, 19.75, 22.32, 30.32, and 30.85 mumol.L-1, respectively. In the PDGF-stimulated proliferation experiment, their IC50 were 9.47, 9.48, 9.25, 12.25, 25.22, and 30.40 mumol.L-1, respectively.


CONCLUSIONS
The six flavonoids inhibited the proliferation of hepatic stellate cells.
Zhang
M
M
Department of Biochemical Pharmacy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China.
Zhang
J P
JP
Ji
H T
HT
Wang
J S
JS
Qian
D H
DH
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D017077:Culture Media, Conditioned; D005260:Female; D005419:Flavonoids / Q000494:pharmacology*; D044948:Flavonols; D006569:Hesperidin; D007728:Kupffer Cells / Q000166:cytology / Q000187:drug effects*; D051379:Mice; D008813:Mice, Inbred ICR; D010982:Platelet-Derived Growth Factor / Q000037:antagonists & inhibitors; D011794:Quercetin / Q000494:pharmacology
D017077:Culture Media, Conditioned; D005419:Flavonoids; D044948:Flavonols; D010982:Platelet-Derived Growth Factor; D011794:Quercetin; D006569:Hesperidin; C017875:fisetin; C013015:hesperetin