Cloning and characterization of the cDNA for a plastid sigma factor from the moss Physcomitrella patens.
We isolated a cDNA PpSig1 encoding a plastid sigma factor from the moss Physcomitrella patens. The PpSIG1 protein is composed of the conserved subdomains for recognition of -10 and -35 promoter elements, core complex binding and DNA melting. Southern blot analysis showed that the moss sig1 gene is likely a member of a small gene family. Transient expression assay using green fluorescent protein demonstrated that the N-terminal region of PpSIG1 functions as a chloroplast-targeting signal peptide. These observations suggest that multiple nuclear-encoded sigma factors regulate chloroplast gene expression in P. patens.
Hara
K
K
Division of Biological Informatics, Graduate School of Human Informatics, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8601, Japan.
Sugita
M
M
Aoki
S
S
D000595:Amino Acid Sequence; D019068:Bryopsida / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000096:biosynthesis / Q000737:chemistry; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D018087:Plastids / Q000378:metabolism; D021382:Protein Sorting Signals / Q000235:genetics; D016415:Sequence Alignment; D012808:Sigma Factor / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*
D018076:DNA, Complementary; D010940:Plant Proteins; D021382:Protein Sorting Signals; D012808:Sigma Factor; C113096:sigma factor Sig1