Crystallization and preliminary X-ray analysis of the complex of the epsilon-subunit and the central domain of the gamma-subunit from the Escherichia coli ATP synthase.
A complex of the epsilon-subunit and the central domain of the gamma-subunit from the ATP synthase of Escherichia coli has been purified and crystallized and preliminary X-ray analysis has been carried out. The crystals belong to space group C222(1), with unit-cell parameters a = 76.7, b = 176.1, c = 67.1 A (at 100 K). Determination of the structure of this protein complex promises to greatly improve the understanding of energy coupling between the F(0) and F(1) sectors within the enzyme complex.
Rodgers
A
A
Department of Pharmacology/Crystallography Centre, University of Western Australia and Western Australian Institute for Medical Research, Nedlands WA 6907, Australia.
Ewart
G
G
Cox
G
G
Wilce
M
M
D001483:Base Sequence; D003460:Crystallization; D018360:Crystallography, X-Ray; D017931:DNA Primers; D004926:Escherichia coli / Q000201:enzymology*; D010957:Plasmids; D006180:Proton-Translocating ATPases / Q000737:chemistry*
D017931:DNA Primers; D006180:Proton-Translocating ATPases