Carbohydrate binding properties of banana (Musa acuminata) lectin I. Novel recognition of internal alpha1,3-linked glucosyl residues.
Examination of lectins of banana (Musa acuminata) and the closely related plantain (Musa spp.) by the techniques of quantitative precipitation, hapten inhibition of precipitation, and isothermal titration calorimetry showed that they are mannose/glucose binding proteins with a preference for the alpha-anomeric form of these sugars. Both generate precipitin curves with branched chain alpha-mannans (yeast mannans) and alpha-glucans (glycogens, dextrans, and starches), but not with linear alpha-glucans containing only alpha1,4- and alpha1,6-glucosidic bonds (isolichenan and pullulan). The novel observation was made that banana and plantain lectins recognize internal alpha1,3-linked glucosyl residues, which occur in the linear polysaccharides elsinan and nigeran. Concanavalin A and lectins from pea and lentil, also mannose/glucose binding lectins, did not precipitate with any of these linear alpha-glucans. This is, the authors believe, the first report of the recognition of internal alpha1,3-glucosidic bonds by a plant lectin. It is possible that these lectins are present in the pulp of their respective fruit, complexed with starch.
Mo
H
H
Department of Biological Chemistry, The University of Michigan Medical School, USA.
Winter
H C
HC
Van Damme
E J
EJ
Peumans
W J
WJ
Misaki
A
A
Goldstein
I J
IJ
D001665:Binding Sites; D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000737:chemistry; D011232:Chemical Precipitation; D002846:Chromatography, Affinity; D005638:Fruit / Q000737:chemistry / Q000378:metabolism*; D037102:Lectins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D037121:Plant Lectins
D002241:Carbohydrates; D037102:Lectins; D037121:Plant Lectins