[Isolation and purification of the active principle from agkistrodon acutus snake venom by high performance ion-exchange liquid chromatography].
The active component from Agkistrodon acutus snake venom was further purified by high performance ion-exchange liquid chromatography over a Protein-PAK DEAE-8HR column(10.0 mm i.d. x 100 mm). The elution was operated with a linear gradient from 10% to 100% NaCl-0.02 mol/L Tris-HCl buffer(pH 8.0). The quantitative results were calculated as 45.41% based on peak area nomalization and the purity of the active component was 90% determined by electrophoresis.
Qian
Y
Y
Department of Biology, University of Science and Technology of China, Hefei, 230027.
Shou
X
X
Cheng
X
X
Wang
C
C
D017836:Agkistrodon; D000721:Ancrod / Q000032:analysis*; D000818:Animals; D002852:Chromatography, Ion Exchange / Q000379:methods; D003435:Crotalid Venoms / Q000737:chemistry*
D003435:Crotalid Venoms; D000721:Ancrod