Histone acetylation induced by granulocyte colony-stimulating factor in a map kinase-dependent manner.
Histone acetylation has been shown to affect chromatin structure and gene expression. The mitogen-activated protein (MAP) kinase pathway is activated by a number of cytokines and plays critical roles in hematopoietic cell survival, proliferation, and differentiation. We focused on the part of the MAP kinase cascade and granulocyte colony-stimulating factor (G-CSF)in histone acetylation at one of the critical myeloid differentiation-associated genes, myeloperoxidase (MPO). G-CSF caused rapid acetylation of histone H3 and H4 at the promoter of MPO as revealed by chromatin immunoprecipitation. In addition, CBP and p300 were recruited to the promoter in response to G-CSF. Furthermore, we showed that rapid histone acetylation induced by G-CSF is MAP kinase-dependent. These results illustrate how myeloid-differentiating signals via G-CSF may be coupled with histone acetylation during the process of gene expression.
Miyata
Y
Y
First Department of Internal Medicine, Nagoya University School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, 466-8550, Japan.
Towatari
M
M
Maeda
T
T
Ozawa
Y
Y
Saito
H
H
D000107:Acetylation; D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation; D002461:Cell Line, Transformed; D017931:DNA Primers / Q000235:genetics; D050881:E1A-Associated p300 Protein; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005419:Flavonoids / Q000494:pharmacology; D015870:Gene Expression; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006657:Histones / Q000378:metabolism*; D051379:Mice; D020928:Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D009687:Nuclear Proteins / Q000378:metabolism; D009195:Peroxidase / Q000235:genetics; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D015534:Trans-Activators / Q000378:metabolism
D017931:DNA Primers; D004791:Enzyme Inhibitors; D005419:Flavonoids; D006657:Histones; D009687:Nuclear Proteins; D012333:RNA, Messenger; D015534:Trans-Activators; D016179:Granulocyte Colony-Stimulating Factor; D009195:Peroxidase; D050881:E1A-Associated p300 Protein; C494361:Ep300 protein, mouse; D020928:Mitogen-Activated Protein Kinases; C093973:2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one