Chlorophyll fluorescence as a bioindicator of effects on growth in aquatic macrophytes from mixtures of polycyclic aromatic hydrocarbons.
Chlorophyll-a fluorescence induction is a rapid technique for measuring photosynthetic electron transport in plants. To assess chlorophyll-a fluorescence as a bioindicator of effects of polycyclic aromatic hydrocarbon mixtures, chlorophyll-a fluorescence parameters and plant growth responses to exposure to the wood preservative creosote were examined in the aquatic plants Lemna gibba and Myriophyllum spicatum. Exposure to creosote inhibited growth of L. gibba (EC50 = 7.2 mg/L total polycyclic aromatic hydrocarbons) and M. spicatum (EC50 = 2.6 mg/L) despite differences in physiology. Creosote also diminished maximum PSII efficiency (Fv/Fm) (EC50 = 36 and 13 mg/L for L. gibba and M. spicatum) and the effective yield of photosystem II photochemistry (deltaF/Fm') (EC50 = 13 and 15 mg/L for L. gibba and M. spicatum). The similarity between growth and chlorophyll-a fluorescence EC50s and slopes of the response curves suggests a close mechanistic link between these end points. The predictive power of chlorophyll-a fluorescence as a bioindicator of whole-organism effects applied to complex contaminant mixtures is discussed.
Marwood
C A
CA
Department of Biology, University of Waterloo, Ontario, Canada.
Solomon
K R
KR
Greenberg
B M
BM
D002734:Chlorophyll / Q000032:analysis*; D000077194:Chlorophyll A; D003407:Creosote / Q000633:toxicity*; D004784:Environmental Monitoring / Q000379:methods; D005618:Fresh Water / Q000032:analysis; D019684:Magnoliopsida / Q000187:drug effects* / Q000254:growth & development; D010777:Photochemistry / Q000379:methods; D018515:Plant Leaves / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis / Q000633:toxicity*; D012044:Regression Analysis; D013050:Spectrometry, Fluorescence / Q000379:methods; D013472:Sunlight; D014466:Ultraviolet Rays; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000633:toxicity*
D011084:Polycyclic Aromatic Hydrocarbons; D014874:Water Pollutants, Chemical; D002734:Chlorophyll; D003407:Creosote; D000077194:Chlorophyll A