title: Chemical force microscopy with active enzymes.
abstract: The adhesion forces have been measured between an atomic force microscope tip derivatized with an active enzyme, shikimate kinase, and an ATP mimic immobilized on a gold surface. Experiments with competitive binding of other ligands in solution show that the observed adhesion forces arise predominantly from specific interactions between the immobilized enzyme and surface-bound adenine derivative. These experiments represent a step in the development of a screening methodology based upon chemical force microscopy.
authors.affiliation: Department of Chemistry, University of Cambridge, Cambridge CB2 1EW, United Kingdom.
authors.forename: M
authors.initials: M
authors.lastname: Fiorini
authors.forename: R
authors.initials: R
authors.lastname: McKendry
authors.forename: M A
authors.initials: MA
authors.lastname: Cooper
authors.forename: T
authors.initials: T
authors.lastname: Rayment
authors.forename: C
authors.initials: C
authors.lastname: Abell
mesh_terms: D001667:Binding, Competitive; D002384:Catalysis; D002448:Cell Adhesion; D004798:Enzymes / Q000737:chemistry*; D006046:Gold / Q000737:chemistry; D007700:Kinetics; D008024:Ligands; D018625:Microscopy, Atomic Force / Q000379:methods*; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000737:chemistry; D011485:Protein Binding; D012765:Shikimic Acid / Q000378:metabolism; D013055:Spectrophotometry, Infrared; D020349:Surface Plasmon Resonance; D013997:Time Factors
chemical_list: D004798:Enzymes; D008024:Ligands; D012765:Shikimic Acid; D006046:Gold; D017853:Phosphotransferases (Alcohol Group Acceptor); C022642:shikimate kinase