Carcinogenicity assays of wood dust and wood additives in rats exposed by long-term inhalation.
OBJECTIVE
This study evaluates whether wood dust and/or wood preservatives develop a carcinogenic potential against the tissues of the airways of rats.


METHODS
The formation of tumors of the respiratory tract after exposure to wood dust was studied in six groups of approximately 60 female Fischer 344 rats exposed by long-term inhalation to mean concentrations of (1) 18 mg/m3 of untreated oak wood dust, (2) wood preservatives containing ca. 1 microgram/m3 lindane and 0.2 microgram/m3 of pentachlorophenol (PCP) in the exposure air, or lindane and 18 micrograms/m3 of PCP (group lindane/PCP vapors, and group oak wood treated with lindane/PCP), (3) 21 or 39 micrograms/m3 of sodium dichromate (calculated as CrO3, group chromate aerosol and group oak wood with chromate), and 72 micrograms/m3 of N-nitrosodimethylamine vapors as positive control. The negative control group consisted of 115 animals (sham-exposed).


RESULTS
Tumors of the nasal cavity developed in two rats exposed to chromate aerosol or in combination with wood dust (2/102, 2%). Malignant tumors of the lower respiratory tract were induced only in exposed groups of rats (three adenocarcinomas of the lung and four bronchiolar lung carcinomas, 7/254, 2.8%). More respiratory tract tumors were observed in rats exposed to chromate or wood with chromate (5/102, 5%), also in groups exposed to oak wood dust (oak untreated, oak + chromate, oak + lindane/PCP; together 5/155, 3.2%). Analysis of 'unpreserved' oak wood dust revealed up to 5 micrograms/m3 of chromate. When this exposure was taken into account, eight of nine animals with respiratory tract tumors (including nasal cavity) had exposure to chromate, while only one tumor occurred in the group lindane/PCP. Otherwise the incidence of systemic tumors was increased in animals exposed to lindane/PCP, due in particular to a significantly increased incidence of liver tumors (OR = 3.7; 1.24-11.3; P = 0.019). Fatal (mucoepidermoid) tumors were induced by N-nitrosodimethylamine (NDMA) in the positive control (14/46, 30%). No such tumors of the respiratory tract were observed in the negative control.


CONCLUSIONS
Tumors in the respiratory tract were found only in exposed animals, predominantly in the groups which inhaled oak wood dust and chromate stain. Chromate may play a decisive role for the etiology of tumors of the nasal cavity in wood workers. This assumption should be supported by further dose-response studies.
Klein
R G
RG
Division of Toxicology and Cancer Risk Factors, German Cancer Research Center, Im Neuenheimer Feld 280, 69120 Heidelberg, Germany. r.klein@dkfz-heidelberg.de
Schmezer
P
P
Amelung
F
F
Schroeder
H G
HG
Woeste
W
W
Wolf
J
J
D000393:Air Pollutants / Q000633:toxicity*; D000818:Animals; D015197:Carcinogenicity Tests; D002840:Chromates / Q000633:toxicity*; D004391:Dust / Q000032:analysis*; D005260:Female; D001556:Hexachlorocyclohexane / Q000633:toxicity*; D008175:Lung Neoplasms / Q000139:chemically induced / Q000473:pathology; D009669:Nose Neoplasms / Q000139:chemically induced / Q000473:pathology; D016017:Odds Ratio; D010416:Pentachlorophenol / Q000633:toxicity*; D051381:Rats; D011916:Rats, Inbred F344; D012142:Respiratory Tract Neoplasms / Q000139:chemically induced / Q000473:pathology; D016019:Survival Analysis; D014934:Wood
D000393:Air Pollutants; D002840:Chromates; D004391:Dust; D001556:Hexachlorocyclohexane; C016104:sodium bichromate; D010416:Pentachlorophenol