title: Pulmonary surfactant phosphatidylcholine transport bypasses the brefeldin A sensitive compartment of alveolar type II cells.
abstract: Brefeldin A (BFA) causes disassembly of the Golgi apparatus and blocks protein transport to this organelle from the endoplasmic reticulum. However, there still remains considerable ambiguity regarding the involvement of the Golgi apparatus in glycerolipid transport pathways. We examined the effects of BFA upon the intracellular translocation of phosphatidylcholine in alveolar type II cells, that synthesize, transport, store and secrete large amounts of phospholipid for regulated exocytosis. BFA at concentrations as high as 10 microg/ml failed to alter the assembly of phosphatidylcholine into lamellar bodies, the specialized storage organelles for pulmonary surfactant. The same concentration of BFA was also ineffective at altering the secretion of newly synthesized phosphatidylcholine from alveolar type II cells. In contrast, concentrations of the drug of 2.5 microg/ml completely arrested newly synthesized lysozyme secretion from the same cells, indicating that BFA readily blocked protein transport processes in alveolar type II cells. The disassembly of the Golgi apparatus in alveolar type II cells following BFA treatment was also demonstrated by showing the redistribution of the resident Golgi protein MG-160 to the endoplasmic reticulum. These results indicate that intracellular transport of phosphatidylcholine along the secretory pathway in alveolar type II cells proceeds via a BFA insensitive route and does not require a functional Golgi apparatus.
authors.affiliation: National Jewish Medical and Research Center, 1400 Jackson St., Denver, CO 80206, USA.
authors.forename: K
authors.initials: K
authors.lastname: Osanai
authors.forename: R J
authors.initials: RJ
authors.lastname: Mason
authors.forename: D R
authors.initials: DR
authors.lastname: Voelker
mesh_terms: D000818:Animals; D000935:Antifungal Agents / Q000494:pharmacology*; D001692:Biological Transport; D020126:Brefeldin A / Q000494:pharmacology*; D002478:Cells, Cultured; D006056:Golgi Apparatus / Q000187:drug effects / Q000378:metabolism; D008297:Male; D009113:Muramidase / Q000378:metabolism; D010713:Phosphatidylcholines / Q000032:analysis / Q000378:metabolism*; D011650:Pulmonary Alveoli / Q000187:drug effects* / Q000378:metabolism; D011663:Pulmonary Surfactants / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000935:Antifungal Agents; D010713:Phosphatidylcholines; D011663:Pulmonary Surfactants; D020126:Brefeldin A; D009113:Muramidase