title: Analytical method to examine the effects of carbon dioxide lasers on skin: a study using wrinkles induced in hairless mice.
abstract: OBJECTIVE
  Investigation of the wrinkle smoothing process elicited by CO(2) laser treatment is important for understanding the mechanism involved in their repair.
  
  
  METHODS
  Hairless mice with wrinkles induced in their dorsal skin by long-term exposure to ultraviolet radiation in the wavelength range of 290-320 nm were treated with a CO(2) laser. By using this model, we investigated the external appearance, histologic changes, and the mechanical properties of the skin during the wrinkle repair.
  
  
  RESULTS
  Laser treatment with an appropriate intensity caused wrinkles to smooth completely. In the healing process, reepithelialization and collagen tissue regeneration in the upper dermis was observed. However, marked changes in the skin were noted, such as increases in the collagen layer and in the skin thickness, and changes in the mechanical properties of the skin, despite the favorable external appearance.
  
  
  CONCLUSIONS
  An abnormal state characterized by excessive collagen regeneration and other changes in the dermis occur concomitantly with wrinkle smoothing.
authors.affiliation: Biological Science Laboratories, Kao Corporation, Tochigi, Japan. 301620@kastanet.kao.co.jp
authors.forename: T
authors.initials: T
authors.lastname: Fujimura
authors.forename: Y
authors.initials: Y
authors.lastname: Takema
authors.forename: S
authors.initials: S
authors.lastname: Moriwaki
authors.forename: K
authors.initials: K
authors.lastname: Tsukahara
authors.forename: G
authors.initials: G
authors.lastname: Imokawa
authors.forename: A
authors.initials: A
authors.lastname: Yamada
authors.forename: S
authors.initials: S
authors.lastname: Imayama
mesh_terms: D000818:Animals; D003094:Collagen / Q000502:physiology; D062109:Dermatologic Surgical Procedures; D004548:Elasticity; D005260:Female; D053685:Laser Therapy; D051379:Mice; D008812:Mice, Hairless; D008813:Mice, Inbred ICR; D015595:Skin Aging; D012879:Skin Physiological Phenomena; D013997:Time Factors
chemical_list: D003094:Collagen