Kinetic analysis of the effect of poliovirus receptor on viral uncoating: the receptor as a catalyst.
We examined the role of soluble poliovirus receptor on the transition of native poliovirus (160S or N particle) to an infectious intermediate (135S or A particle). The viral receptor behaves as a classic transition state theory catalyst, facilitating the N-to-A conversion by lowering the activation energy for the process by 50 kcal/mol. In contrast to earlier studies which demonstrated that capsid-binding drugs inhibit thermally mediated N-to-A conversion through entropic stabilization alone, capsid-binding drugs are shown to inhibit receptor-mediated N-to-A conversion through a combination of enthalpic and entropic effects.
Tsang
S K
SK
Committee on Higher Degrees in Biophysics, Harvard University, Cambridge, Massachusetts 02138, USA.
McDermott
B M
BM
Racaniello
V R
VR
Hogle
J M
JM
D000998:Antiviral Agents / Q000494:pharmacology*; D002213:Capsid / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D007700:Kinetics; D008962:Models, Theoretical; D017955:Poliovirus / Q000187:drug effects / Q000502:physiology*; D011991:Receptors, Virus / Q000378:metabolism / Q000502:physiology*
D000998:Antiviral Agents; D011991:Receptors, Virus