Luminol-dependent chemiluminescence analysis of chitooligosaccharide-induced rapid production of hydrogen peroxide by intact wheat seedlings.
The feasibility of a luminol-dependent chemiluminescence analysis of hydrogen peroxide production by intact wheat seedlings using a KhL-003 chemiluminometer was determined. It was shown that the minimal H2O2 concentration that can be detected in a 0.5-ml sample with this instrument is 0.125 microM. Analysis of biological activity of a mixture of chitooligosaccharides with molecular masses from 5 to 10 kD and acetylation degree of 65% demonstrated that, at a concentration of 1 microg/ml, they induce rapid overproduction of H2O2 in roots of 3-day-old wheat seedlings.
Khairullin
R M
RM
Institute of Biochemistry and Genetics, Ufa Scientific Center, Russian Academy of Sciences, Ufa, 450054, Russia. phyto@anrb.ru
Akhmetova
I E
IE
D002686:Chitin / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D006861:Hydrogen Peroxide / Q000378:metabolism*; D007202:Indicators and Reagents / Q000494:pharmacology*; D008163:Luminescent Measurements; D008165:Luminol / Q000494:pharmacology*; D010100:Oxygen / Q000378:metabolism; D013997:Time Factors; D014908:Triticum / Q000378:metabolism*
D007202:Indicators and Reagents; D002686:Chitin; D008165:Luminol; D006861:Hydrogen Peroxide; D010100:Oxygen