Characterization of the prostaglandin receptors in human osteoblasts in culture.
Prostaglandins have complex actions on bone metabolism that depend on interactions with different types and subtypes of receptors. Our objective was to characterize the prostaglandins receptors present in primary cultures of human osteoblasts. RT-PCR analysis revealed the presence of DP, EP(4), IP, FP and TP receptor mRNA in primary cultures of human osteoblasts. FP receptor mRNA was detected only after 3 weeks of confluency, all the others were detected at every culture time tested. To verify the functionality of these receptors we challenged the cells with the prostanoids and synthetic analogues and determined the intracellular levels of cAMP. All receptors found by RT-PCR were coupled to second messengers except for the DP subtype. These results clearly show the presence of functional EP(4), IP, FP and TP receptors in human osteoblasts in culture.
Sarrazin
P
P
Department of Medicine, Faculty of Medicine, Université de Sherbrooke, Sherbrooke, Canada.
Bkaily
G
G
Haché
R
R
Patry
C
C
Dumais
R
R
Rocha
F A
FA
de Brum-Fernandes
A J
AJ
D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D010006:Osteoblasts / Q000378:metabolism*; D015675:Osteocalcin / Q000096:biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D011982:Receptors, Prostaglandin / Q000096:biosynthesis* / Q000737:chemistry* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors
D012333:RNA, Messenger; D011982:Receptors, Prostaglandin; D015675:Osteocalcin; D000242:Cyclic AMP; D002118:Calcium