The Aplysia californica Cl- pump is a P-type ATPase: evidence through inhibition studies.
Utilizing a proteoliposomal preparation containing Cl(-)-ATPase from Aplysia californica foregut, it was shown that orthovanodate inhibited Cl(-)-ATPase activity, ATP-dependent Cl- transport, ATP-dependent membrane potential change and ATP-dependent phosphorylation. N-ethylmalemide and p-chloromercurobenzoate also inhibited the Cl- pump biochemical and physiological transport characteristics. However, bafilomycin, azide, N, N'-dicyclohexylcarboiimide (DCCD), and efrapeptin had no effect on the Cl- pump biochemical or physiological characteristics, suggesting that this Cl- pump was a P-type ATPase. It was concluded that this P-type ATPase Cl- pump is the mechanism that is responsible for the net absorptive flux of Cl- in the A. californica foregut.
Gerencser
G A
GA
Department of Physiology, College of Medicine, University of Florida, Gainesville 32610, USA.
Zhang
J
J
D002731:4-Chloromercuribenzenesulfonate / Q000494:pharmacology; D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000818:Animals; D027321:Anion Transport Proteins; D001048:Aplysia / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D018118:Chloride Channels / Q000037:antagonists & inhibitors / Q000378:metabolism*; D002712:Chlorides / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005033:Ethylmaleimide / Q000494:pharmacology; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D010766:Phosphorylation / Q000187:drug effects; D011510:Proteolipids / Q000187:drug effects / Q000378:metabolism; D014638:Vanadates / Q000494:pharmacology
D027321:Anion Transport Proteins; D018118:Chloride Channels; D002712:Chlorides; D004791:Enzyme Inhibitors; D011510:Proteolipids; C020485:proteoliposomes; D014638:Vanadates; D002731:4-Chloromercuribenzenesulfonate; D000251:Adenosine Triphosphatases; C031652:anion-sensitive ATPases; D005033:Ethylmaleimide