title: Expansion of CD4+CD16+ blood monocytes in patients with chronic renal failure undergoing dialysis: possible involvement of macrophage colony-stimulating factor.
abstract: A novel subpopulation of blood monocytes coexpressing CD16 antigen and low levels of CD14 antigen (CD14+CD16+ monocytes) has recently been identified, and expansion of these CD14+CD16+ monocytes has been reported under some pathological conditions. In this study, we examined the immunophenotype of blood monocytes in patients with chronic renal failure (CRF) who were undergoing hemodialysis (HD, n = 52) or continuous ambulatory peritoneal dialysis (CAPD, n = 36) using two-color immunofluorescence flow cytometry. The percentage and absolute number of CD14+CD16+ monocytes were significantly higher (p < 0.001) in both HD and CAPD patients compared with those in healthy control subjects. We also determined the plasma concentrations of hematopoietic growth factors and cytokines using an enzyme-linked immunosorbent immunoassay. The plasma levels of macrophage colony-stimulating factor (M-CSF) were markedly increased in both HD and CAPD patients relative to the normal controls. The plasma M-CSF levels correlated significantly with the number of CD14+CD16+ monocytes in the whole group of subjects. These findings suggest that elevated endogenous M-CSF levels may participate in the expansion of CD14+CD16+ monocytes in CRF patients undergoing dialysis.
authors.affiliation: Department of Laboratory Medicine, National Defense Medical College, Saitama, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Saionji
authors.forename: A
authors.initials: A
authors.lastname: Ohsaka
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016207:Cytokines / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D016298:Hematopoietic Cell Growth Factors / Q000097:blood; D006801:Humans; D016130:Immunophenotyping; D007676:Kidney Failure, Chronic / Q000097:blood* / Q000628:therapy; D007958:Leukocyte Count; D018950:Lipopolysaccharide Receptors / Q000032:analysis*; D016173:Macrophage Colony-Stimulating Factor / Q000097:blood; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000276:immunology* / Q000473:pathology*; D010531:Peritoneal Dialysis, Continuous Ambulatory; D017452:Receptors, IgG / Q000032:analysis*; D006435:Renal Dialysis
chemical_list: D016207:Cytokines; D016298:Hematopoietic Cell Growth Factors; D018950:Lipopolysaccharide Receptors; D017452:Receptors, IgG; D016173:Macrophage Colony-Stimulating Factor