Comparison of some different methods for analysis of thyroid autoantibodies: importance of thyroglobulin autoantibodies.
Blood samples from 141 children and adolescents were used to evaluate differences between commercial kits and radioimmunoassay (RIA) methods for detecting thyroid autoantibodies. Thyroglobulin autoantibodies (Tg-Ab) were analyzed with a hemagglutination kit and a RIA; thyroid peroxidase autoantibodies (TPO-Ab) were measured with a gelagglutination assay and a RIA. The results of the antibody tests were compared with thyroid function tests (triiodothyronine [T3], thyroxine [T4], thyrotropin [TSH]) and with the results of ultrasound of the thyroid in antibody-positive patients. The correlation of antibody levels between the two methods was higher for TPO-Ab than for Tg-Ab. Moderate to high levels of TPO-Ab correlated to elevated TSH levels. Autoimmune thyroiditis (AIT) was found in 6 of the 141 children. The RIA-based thyroglobulin assay was the only test that identified autoantibodies in all 6 cases. In contrast, the hemagglutination kit thyroglobulin assay failed to identify 4 of the 6 AIT cases.
Lindberg
B
B
Department of Pediatrics, University of Lund, University Hospital, Malmö, Sweden. bengt.lindberg@pediatrik.mas.lu.se
Svensson
J
J
Ericsson
U B
UB
Nilsson
P
P
Svenonius
E
E
Ivarsson
S A
SA
D000293:Adolescent; D000328:Adult; D000372:Agglutination Tests; D001323:Autoantibodies / Q000097:blood*; D001707:Biopsy, Needle; D002648:Child; D002675:Child, Preschool; D005188:False Negative Reactions; D005260:Female; D006386:Hemagglutination Tests; D006801:Humans; D007453:Iodide Peroxidase / Q000276:immunology; D008297:Male; D011863:Radioimmunoassay; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D013954:Thyroglobulin / Q000276:immunology*; D013961:Thyroid Gland / Q000000981:diagnostic imaging / Q000276:immunology* / Q000473:pathology; D013967:Thyroiditis, Autoimmune / Q000175:diagnosis / Q000276:immunology; D013972:Thyrotropin / Q000097:blood; D013974:Thyroxine / Q000097:blood; D014284:Triiodothyronine / Q000097:blood; D014463:Ultrasonography
D001323:Autoantibodies; D011933:Reagent Kits, Diagnostic; D014284:Triiodothyronine; D013972:Thyrotropin; D013954:Thyroglobulin; D007453:Iodide Peroxidase; D013974:Thyroxine