Effects of blockade of cerebral lymphatic drainage on cerebral ischemia after middle cerebral artery occlusion in rats.
This experiment aimed to investigate the effects of blockade of cerebral lymphatic drainage on cerebral ischemic damage. Seventy six Wistar rats were divided randomly into middle cerebral artery occlusion (MCAO) group and MCAO plus cerebral lymphatic blockade (MCAO+CLB) group for the experiment. The contents of water and electrolytes, the activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) in the ischemic brain tissue were detected at 24, 48 and 72 hours after the operation. The morphologic examination was also performed. In MCAO group, contents of water, sodium and calcium in the ischemic brain tissue increased significantly at any time after the operation. The SOD activity decreased while the MDA content increased markedly. The morphologic findings showed severe damage of ischemic brain tissue and neurons. In MCAO+CLB group, the above parameters were altered more obviously. The present observation suggests that blockade of cerebral lymphatic drainage may deteriorate ischemic brain damage after MCAO.
Sun
B L
BL
Institute of Microcirculation, Affiliated Hospital of Taishan Medical College, Taian, Shandong, China. sunbl88@263.net
Xia
Z L
ZL
Yan
Z W
ZW
Chen
Y S
YS
Yang
M F
MF
D000818:Animals; D001834:Body Water; D001923:Brain Chemistry; D002545:Brain Ischemia / Q000209:etiology / Q000378:metabolism*; D002118:Calcium / Q000032:analysis; D003250:Constriction; D005260:Female; D005609:Free Radicals; D020244:Infarction, Middle Cerebral Artery / Q000150:complications / Q000378:metabolism*; D008208:Lymphatic System / Q000503:physiopathology*; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D009419:Nerve Tissue Proteins / Q000032:analysis; D009474:Neurons / Q000737:chemistry / Q000473:pathology; D018384:Oxidative Stress; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D012964:Sodium / Q000032:analysis; D013482:Superoxide Dismutase / Q000032:analysis
D005609:Free Radicals; D009419:Nerve Tissue Proteins; D017382:Reactive Oxygen Species; D008315:Malondialdehyde; D012964:Sodium; D013482:Superoxide Dismutase; D002118:Calcium