title: [Co-integration of BLG-LAtPA and WAP improved the expression of LAtPA in transgenic mouse milk].
abstract: In order to improve the expression of longer acting tissue plasminogen activator in the mammary epithelium of transgenic mice, the fragment of BLG-LAtPA hydrid gene was microinjected into mouse embryos with mice whey acid protein gene. Three mouse were tested as being Co-integration of BLG-LAtPA and WAP transgene by PCR and Southern blot. Milk obtained from lactating females contains biologically active tPA, and the concentration of tPA was calculated to be about 10 micrograms/mL.
authors.affiliation: Institute of Beijing Biotechnology, Beijing 100071, China.
authors.forename: J
authors.initials: J
authors.lastname: Zhou
authors.forename: J X
authors.initials: JX
authors.lastname: Deng
authors.forename: X
authors.initials: X
authors.lastname: Cheng
authors.forename: Y F
authors.initials: YF
authors.lastname: Lu
authors.forename: X
authors.initials: X
authors.lastname: Yang
authors.forename: P T
authors.initials: PT
authors.lastname: Huang
mesh_terms: D000818:Animals; D005260:Female; D007782:Lactoglobulins / Q000235:genetics*; D051379:Mice; D008822:Mice, Transgenic; D008845:Microinjections; D008892:Milk / Q000378:metabolism*; D008894:Milk Proteins / Q000235:genetics*; D010959:Tissue Plasminogen Activator / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D007782:Lactoglobulins; D008894:Milk Proteins; C024471:whey acidic proteins; D010959:Tissue Plasminogen Activator