title: Identification of the modifying sites of mono-PEGylated salmon calcitonins by capillary electrophoresis and MALDI-TOF mass spectrometry.
abstract: A capillary electrophoretic method (CE) was developed for the determination of the PEG-modification sites of three positional isomers of mono-PEG modified salmon calcitonins (mono-PEG-sCTs). Resistance to proteolytic degradation on the PEG modification sites resulted in different patterns of CE electropherograms for the tryptic digested mono-PEG-sCTs isomers, and the PEG modification sites were assigned accordingly. The PEG-modification sites were also confirmed directly by determining the molecular masses of the tryptic digested PEG-modified fragments of respective mono-PEG-sCT by the matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry.
authors.affiliation: Drug Targeting Laboratory, College of Pharmacy, SungKyunKwan University, Suwon, South Korea.
authors.forename: D H
authors.initials: DH
authors.lastname: Na
authors.forename: M O
authors.initials: MO
authors.lastname: Park
authors.forename: S Y
authors.initials: SY
authors.lastname: Choi
authors.forename: Y S
authors.initials: YS
authors.lastname: Kim
authors.forename: S S
authors.initials: SS
authors.lastname: Lee
authors.forename: S D
authors.initials: SD
authors.lastname: Yoo
authors.forename: H S
authors.initials: HS
authors.lastname: Lee
authors.forename: K C
authors.initials: KC
authors.lastname: Lee
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D002116:Calcitonin / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods; D007536:Isomerism; D008969:Molecular Sequence Data; D011092:Polyethylene Glycols / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014357:Trypsin
chemical_list: D011092:Polyethylene Glycols; C028815:salmon calcitonin; D002116:Calcitonin; D014357:Trypsin