title: Type VIII collagen: heterotrimeric chain association.
abstract: Two chains, alpha1(VIII) and alpha2(VIII), have been described for type VIII collagen. Early work suggested that these chains were present in a 2:1 ratio, although recent work has shown that homotrimers can form and predominate in some tissues. In order to address the question of whether the alpha1(VIII) and alpha2(VIII) chains could co-polymerise we made a shortened alpha1(VIII) chain and expressed this with full length alpha2(VIII) chain in an in vitro translation system supplemented with semi-permeabilised cells. Heterotrimers containing either two or one alpha2(VIII) were evident. Interestingly, a point mutation in the NC1 domain of the alpha1(VIII) chain abrogated trimer formation. In addition we were able to demonstrate chain association of the alpha1(X) chain of type X collagen with the shortened alpha1(VIII) chain. Variations in chain association were seen when altered ratios of message were used. These results demonstrate the importance of the NC1 domain in chain association and suggest that gene expression regulates the composition and function of type VIII collagen by varying chain composition.
authors.affiliation: University of Manchester, Wellcome Trust Center for Cell/Matrix Research, 2.205 Stopford Building, Oxford Rd, M13 9PT, Manchester, UK.
authors.forename: C
authors.initials: C
authors.lastname: Illidge
authors.forename: C
authors.initials: C
authors.lastname: Kielty
authors.forename: A
authors.initials: A
authors.lastname: Shuttleworth
mesh_terms: D001345:Autoradiography; D003094:Collagen / Q000737:chemistry* / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D021122:Protein Subunits; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013696:Temperature; D014407:Tumor Cells, Cultured
chemical_list: D021122:Protein Subunits; D011994:Recombinant Proteins; D003094:Collagen