[Frequency dependence of somatostatin and calcitonin gene related peptide release induced by electroacupuncture in rat spinal cord].
Radioimmunoassay (RIA) was used to determine the changes of the immunoreactivity (ir) of somatostatin (SOM) and calcitonin gene-related peptide (CGRP) in the spinal perfusate of rat induced by electroacupuncture (EA) of different frequencies. The frequency of EA was chosen to be 2, 15 and 100 Hz and the spinal perfusate was collected in three periods of 30 min before, during and after EA. The results indicate: (1) low frequency EA (2 Hz) increased the spinal SOM-ir level by 39% (P < 0.05) but decreased that of CGRP-ir by 47% (P < 0.05); (2) conversely, 15 Hz EA decreased spinal fluid SOM-ir level by 37% (P < 0.05) but increased that of CGRP-ir by 92% (P < 0.05); (3) 100 Hz EA behaved like 15 Hz in inhibiting SOM-ir level (P < 0.01), but without effect on CGRP-ir. The above results show that specific frequency is required for peripheral electrical stimulation to activate or suppress the release of spinal neuropeptides SOM and CGRP, a fact that may have clinical implications.
Tian
J B
JB
Neuroscience Research Center, Beijing Medical University, Beijing 100083.
Shen
S
S
Han
J S
JS
D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000134:cerebrospinal fluid*; D015671:Electroacupuncture; D005260:Female; D008297:Male; D010477:Perfusion; D011863:Radioimmunoassay; D051381:Rats; D017208:Rats, Wistar; D013004:Somatostatin / Q000134:cerebrospinal fluid*; D013116:Spinal Cord / Q000378:metabolism*; D013346:Subarachnoid Space
D013004:Somatostatin; D015740:Calcitonin Gene-Related Peptide