title: Co-adsorbed fibrinogen and von Willebrand factor augment platelet procoagulant activity and spreading.
abstract: Previously we observed that platelets adherent to surfaces preadsorbed with blood plasma exhibited 1.3 to 2.4 times greater procoagulant activity than platelets on surfaces adsorbed with fibrinogen (Fg) only. These observations suggested that the adhesion proteins adsorbed from plasma may activate platelets in a cooperative, or synergistic manner. In the present study, polystyrene surfaces adsorbed with both Fg and vWF induced up to three times greater procoagulant activity than surfaces adsorbed with Fg or vWF only. The amounts of Fg and vWF adsorbed from binary mixtures that resulted in increased procoagulant activity were found to be similar to the amounts that adsorbed to PS from 100% plasma. The effect of adsorbed adhesion proteins on platelet spreading was also investigated. The proportion of fully spread platelets increased, depending on the adhesion protein preadsorbed to the surface, in the following order: vWF < Fg < Fn < (vWF + Fg) < Vn < plasma.
authors.affiliation: Department of Bioengineering, University of Washington, Seattle 98195, USA.
authors.forename: J M
authors.initials: JM
authors.lastname: Grunkemeier
authors.forename: W B
authors.initials: WB
authors.lastname: Tsai
authors.forename: T A
authors.initials: TA
authors.lastname: Horbett
mesh_terms: D001792:Blood Platelets / Q000378:metabolism* / Q000648:ultrastructure; D002448:Cell Adhesion / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D003029:Coagulants / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D005340:Fibrinogen / Q000737:chemistry*; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008855:Microscopy, Electron, Scanning; D010949:Plasma / Q000737:chemistry; D010974:Platelet Aggregation / Q000187:drug effects*; D011137:Polystyrenes / Q000737:chemistry; D013925:Thromboplastin / Q000378:metabolism; D014841:von Willebrand Factor / Q000737:chemistry*
chemical_list: D003029:Coagulants; D011137:Polystyrenes; D014841:von Willebrand Factor; D005340:Fibrinogen; D013925:Thromboplastin; D007770:L-Lactate Dehydrogenase