Effects of emphysema on diaphragm microvascular oxygen pressure.
Pulmonary emphysema impairs lung and respiratory muscle function leading to restricted physical capacity and accelerated morbidity and mortality consequent to respiratory muscle failure. In the absence of direct evidence, an O2 supply-demand imbalance within the diaphragm and other respiratory muscles in emphysema has been considered the most likely explanation for this failure. To test this hypothesis, we utilized phosphorescence quenching techniques to measure mean microvascular PO2 (PO2m) within the medial costal diaphragm of control (C, n = 10) and emphysematous (E, elastase instilled, n = 7) hamsters. PO2m and mean arterial pressure (MAP) were measured in the spontaneously breathing anesthetized hamster at inspired O2 percentages of 10, 21, and 100, and across a range of mean MAPs from 40 to 115 mm Hg. At each inspired O2, diaphragm PO2m was significantly (p < 0.05) lower in E animals (10%: C, 19 +/- 3; E, 9 +/- 2; 21%: C, 32 +/- 2; E, 21 +/- 2; 100%: C, 60 +/- 8; E, 36 +/- 9 mm Hg). At 21% inspired O2, the PO2m decrease was correlated with reduced MAP in both C (r = 0.968) and E (r = 0.976) animals. We conclude that diaphragmatic PO2m (and therefore microvascular O2 content) is decreased in emphysematous hamsters reflecting a greater diaphragmatic O2 utilization at rest and a lower O2 extraction reserve. According to Fick's law, this lower PO2m will mandate an exaggerated fall in intramyocyte PO2, which is expected to accelerate muscle glycogen depletion and consequently fatigue. This provides empirical evidence in support of one possible mechanism for respiratory muscle failure in emphysema.
Poole
D C
DC
Department of Anatomy and Physiology, Kansas State University, Manhattan, Kansas 66506-5602, USA. poole@vet.ksu.edu
Kindig
C A
CA
Behnke
B J
BJ
D000704:Analysis of Variance; D000818:Animals; D006224:Cricetinae; D003964:Diaphragm / Q000098:blood supply*; D018496:Hyperoxia / Q000503:physiopathology; D000860:Hypoxia / Q000503:physiopathology; D008173:Lung Diseases, Obstructive / Q000503:physiopathology; D008652:Mesoporphyrins; D008665:Metalloporphyrins; D008833:Microcirculation; D018763:Muscle Fatigue / Q000502:physiology*; D010100:Oxygen / Q000097:blood*; D010313:Partial Pressure; D011656:Pulmonary Emphysema / Q000503:physiopathology*; D011897:Random Allocation; D012720:Severity of Illness Index
D008652:Mesoporphyrins; D008665:Metalloporphyrins; C079625:palladium-meso-tetra(4-carboxyphenyl)porphine; D010100:Oxygen