Effect of tea polyphenols and tea pigments on the inhibition of precancerous liver lesions in rats.
The objective of this study was to investigate the inhibitory effect of tea components, tea polyphenols and tea pigments, on precancerous liver lesions in rats. A rat liver precancerous lesion model was established by multiple low-dosage N-nitrosodiethylamine (NDEA) injections, followed by intraperitoneal CCl4 injection and partial hepatectomy (PH). Tea pigments (0.1%) or tea polyphenols (0.1%) were given to Wistar rats in drinking water during the eight weeks of the experiment. The number and area of glutathione S-transferase Pi-positive foci in the rat liver were used as biomarkers of precancerous liver lesions. Western and Northern blot techniques were used to detect rat liver GST-Pi expression at the protein and mRNA levels. At the end of the experiment, tea polyphenols and tea pigments significantly decreased the number and area of GST-Pi-positive foci that were overexpressed in the NDEA-CCl4-PH-treated rats compared with the positive control group. The results also showed that GST-Pi mRNA and protein expression increased significantly in the NDEA-CCl4-PH-treated group, which is consistent with the changing of GST-Pi-positive foci. Tea pigments and tea polyphenols had an inhibitory effect on the overexpression of GST-Pi mRNA and protein in NDEA-CCl4-PH-treated rats. These results suggest that tea pigments and tea polyphenols are effective in preventing the occurrence and progression of precancerous liver lesions in rats.
Gong
Y
Y
Institute of Nutrition and Food Hygiene, Chinese Academy of Preventive Medicine, Beijing 100050, China.
Han
C
C
Chen
J
J
D000818:Animals; D015415:Biomarkers; D015152:Blotting, Northern; D015153:Blotting, Western; D004195:Disease Models, Animal; D004326:Drinking; D005419:Flavonoids; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism; D008099:Liver / Q000201:enzymology; D008114:Liver Neoplasms, Experimental / Q000517:prevention & control*; D008297:Male; D010636:Phenols / Q000008:administration & dosage / Q000494:pharmacology*; D010860:Pigments, Biological / Q000008:administration & dosage / Q000494:pharmacology*; D011108:Polymers / Q000008:administration & dosage / Q000494:pharmacology*; D059808:Polyphenols; D011230:Precancerous Conditions / Q000517:prevention & control*; D012333:RNA, Messenger / Q000037:antagonists & inhibitors; D051381:Rats; D017208:Rats, Wistar; D013662:Tea / Q000737:chemistry*
D015415:Biomarkers; D005419:Flavonoids; D010636:Phenols; D010860:Pigments, Biological; D011108:Polymers; D059808:Polyphenols; D012333:RNA, Messenger; D013662:Tea; D005982:Glutathione Transferase