title: Transforming growth factor beta receptor induction in herniated intervertebral disc tissue: an immunohistochemical study.
abstract: Transforming growth factor beta (TGF-beta) is a potent inducer of angiogenesis and fibrogenesis. There is presently little information about the pathophysiological function of TGF-beta in herniated disc tissue. In order to analyze the cellular role and activation of TGF-beta after disc herniation we immunostained frozen material from 38 disc herniation operations and from eight macroscopically normal discs from organ donors. Polyclonal TGF-beta-I, TGF-beta-II and TGF-beta receptor type II antibodies were used with the avidin biotin complex (ABC-) immunoperoxidase method. All the herniated discs were TGF-beta immunopositive. Such immunoreactivity was mainly associated with disc cells. In a few samples, capillaries were also TGF-beta immunopositive. Immunopositivity was similarly observed in the control discs. To analyze possible differences between the two groups, we calculated the ratio of immunopositive disc cells. For all three antibodies, a statistically significantly (Mann-Whitney test, P = 0.0001) higher number of disc cells showed immunopositivity in the herniated discs. The increase in TGF-beta receptor immunopositivity suggested induction of TGF-beta receptors in herniated discs. Our results support an active regulatory role for TGF-beta in disc cell metabolism.
authors.affiliation: Research Laboratory, Spine Research Unit, Department of Orthopaedics and Traumatology, Helsinki University Central Hospital, Helsinki, Finland.
authors.forename: J
authors.initials: J
authors.lastname: Tolonen
authors.forename: M
authors.initials: M
authors.lastname: Grönblad
authors.forename: J
authors.initials: J
authors.lastname: Virri
authors.forename: S
authors.initials: S
authors.lastname: Seitsalo
authors.forename: T
authors.initials: T
authors.lastname: Rytömaa
authors.forename: E
authors.initials: E
authors.lastname: Karaharju
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002196:Capillaries / Q000378:metabolism; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007403:Intervertebral Disc / Q000098:blood supply / Q000378:metabolism / Q000473:pathology; D007405:Intervertebral Disc Displacement / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D018125:Receptors, Transforming Growth Factor beta / Q000378:metabolism*; D012016:Reference Values
chemical_list: D018125:Receptors, Transforming Growth Factor beta