[Hypoglycemic effect of sodium salt of monoketocholic acid in diabetic rats].
BACKGROUND
In recent years experiments have showed that synthetic derivatives of bile acid affect blood glucose level. The aim of this work was to study the influence of sodium salt of monoketcholanate (MKH) on blood glucose level in rats with induced diabetes.


METHODS
Diabetes was induced with streptozotocin applied intraperitoneally in a dose of 70 mg/kg body weight. The experiments were carried out in white laboratory rats. They were divided in two groups: group A--rats with induced diabetes (n = 10) and group B--rats with normal blood glucose level (control group, n = 10). Both groups of animals were anesthetized with urethan applied intraperitoneally in a dose of 0.75 g/kg body weight and were divided in two subgroups. The subgroups, one of each group of animals, were treated intranasally with 2% solution of MHK, in dose of 0.2 ml/kg body weight. The other two subgroups (control subgroups) received saline solution intranasally in a dose of 0.2 ml/kg body weight. Blood was taken periodically from the tail vein and blood glucose level was determined at 0, 15, 30, 45, 60, 90, 120 and 180-th minutes after treatment.


RESULTS
In the group of rats with normal blood glucose level (group B) MKH did not show any statistically important changes in blood glucose level. In group of rats with induced diabetes (group A). MKH significantly decreased blood glucose level at 30, 45, 60 and 90-th minute in comparison to the diabetic rats treated with saline solution.


CONCLUSIONS
The results of the study show that intranasally applied 2% solution of MKH has a hypoglycemic effect, but only in rats with diabetes. The same substance has no influence on blood glucose level in normoglycemic rats.
Tenji
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Medicinski fakultet, Zavod za farmakologiju, toksikologiju i klinicku farmakologiju, Univerzitet u Novom Sadu.
D000818:Animals; D001786:Blood Glucose / Q000187:drug effects*; D002793:Cholic Acids / Q000494:pharmacology*; D003921:Diabetes Mellitus, Experimental / Q000097:blood*; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D001786:Blood Glucose; D002793:Cholic Acids; D007004:Hypoglycemic Agents