title: Rice alpha-mannosidase digesting the high mannose glycopeptide of glutelin.
abstract: alpha-Mannosidase (EC 3.2.1.24) from rice dry seeds was purified to homogeneity. Optimum pH and Km for pNP-alpha-Man hydrolysis were pH 4.3-4.5 and 1.04 mM, respectively. The enzyme digested mannobioses such as Manalpha-1,2Man, Manalpha-1,6Man, Manalpha-1,3Man but Manalpha-1,4Man. Zn2+ ion was required for the activity, whereas EDTA and swainsonine inhibited the activity by 80 and 96%, respectively. The rice storage protein, glutelin was prepared and its basic subunits were shown to have high mannose-type sugar chains by two-dimensional mapping using NH2-P and C18 silica columns. They were Man9GlcNAc2, Man8GlcNAc2, Man7GlcNAc2, Man6GlcNAc2 and Man5GlcNAc2. All these oligosaccharides were digested by the purified alpha-mannosidase, and Man-GlcNAc2 and mannose were formed. Glycopeptides, having these high mannose-type sugar chains, could also be digested by the alpha-mannosidase. Subunits were prepared from glutelin basic subunit and the richest subunit among them, subunit 2 (isoform 2), was digested by the alpha-mannosidase. Isoform 2 was digested by V8 protease only partially and slowly. However, isoform 2, pre-treated with the alpha-mannosidase, was rapidly and completely digested by V8 protease.
authors.affiliation: Graduate School of Natural Science and Technology, Niigata University, Ikarashi, Niigata 950-2181, Japan.
authors.forename: Tadashi
authors.initials: T
authors.lastname: Kishimoto
authors.forename: Hidetaka
authors.initials: H
authors.lastname: Hori
authors.forename: Daisuke
authors.initials: D
authors.lastname: Takano
authors.forename: Yoshiyasu
authors.initials: Y
authors.lastname: Nakano
authors.forename: Mayumi
authors.initials: M
authors.lastname: Watanabe
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Mitsui