title: [Value and limits of microscopy of exudates in Mycobacterium ulcerans cutaneous infection in Côte d'Ivoire].
abstract: The objective of this study was to estimate the interest and the limits of skin exudate microscopy for patients with chronic ulceration with clinically suspected Buruli's ulcer and living in an endemic area in Côte d'Ivoire. Two stained smears, one with Ziehl-Neelsen and the other Dugomier staining, were produced from 140 samples obtained after a swab of skin lesions. The positive smear rate for the acid fast bacilli (AFB) was respectively 16.4% and 12.9% for Dugomier and Ziehl-Neelsen staining. The ulceration with less than one year of evolution have high AFB rates of 15.8% for Ziehl-Neelsen staining and 21.1% for Dugomier's. Microscopic examination of skin exudate in face of ulceration, clinically suspected to be a Buruli's ulcer is not the best tool for biological diagnosis, due to poor technical sensibility. Nevertheless, it remains a good first means of investigation in an endemoepidemic area.
authors.affiliation: Laboratoire de bactériologie-virologie de l'Institut Pasteur, 01BP 490, Abidjan 01, Côte d'Ivoire.
authors.forename: K
authors.initials: K
authors.lastname: N'Guessan
authors.forename: Y
authors.initials: Y
authors.lastname: Kouassi
authors.forename: S
authors.initials: S
authors.lastname: Bouzid
authors.forename: P
authors.initials: P
authors.lastname: Ehuie
authors.forename: K
authors.initials: K
authors.lastname: Koffi
authors.forename: C
authors.initials: C
authors.lastname: Oniangue
authors.forename: N
authors.initials: N
authors.lastname: Aka
authors.forename: M
authors.initials: M
authors.lastname: Dosso
mesh_terms: D007560:Cote d'Ivoire; D005122:Exudates and Transudates / Q000382:microbiology*; D006801:Humans; D009165:Mycobacterium Infections, Nontuberculous / Q000175:diagnosis*; D019911:Mycobacterium ulcerans / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D012874:Skin Diseases, Infectious / Q000382:microbiology*