Total antioxidant levels, gender, and age as risk factors for DNA damage in lymphocytes of the elderly.
During past years, the association of oxidative stress with DNA damage and its possible clinical translation into chronic degenerative illnesses, such as atherosclerosis, cancer, diabetes mellitus and Alzheimer's disease, has been demonstrated. In addition, it has been pointed out that age and gender are factors that influence the generation of DNA damage; however, this is still controversial. We have previously reported the results of a study of 88 subjects older than 60 years of age in whom DNA damage is related with serum levels of total antioxidants. The results of this study demonstrate a greater frequency of DNA damage in elderly persons with normal levels of antioxidants, in addition to males, and in the younger group of subjects, i.e., 60-69 years. In this work, we enlarged our study sample to 160 elderly subjects; in this way, we were able to evaluate the consistency of the influence of total antioxidants, age, and gender on the magnitude and grade of DNA damage in lymphocytes of the elderly. The results demonstrated that 45% of the subjects showed DNA damage, measured by an alkaline unicellular electrophoresis technique (comet assay). Similarly, 62% of the subjects presented low levels of total antioxidant levels measured by a colorimetric method (Randox Kit). A greater percentage of DNA damage was observed in subjects with normal levels of antioxidants (48%) compared with subjects with low levels (43%), although the difference was not statistically significant. The group of subjects 70 years of age or older showed a greater percentage of DNA damage (50%) than the group of subjects of 60-69 years of age (41%). However, the difference was again not statistically significant (P>0.05). With respect to gender, 64% of males and 38% of females had DNA damage with an odds ratio (OR) of 2.86 and a 95% confidence interval (CI) of 1.31-6.32 (P<0.05). In the logistic regression analysis, the interaction of the male sex variables with low antioxidants had an OR of 2.5 (CI 95%, 1.33-4.68; P<0.01). We conclude that the interaction of male sex factors-low levels of antioxidants would justify the indication of antioxidant dietetic supplements.
Mendoza-Núñez
V M
VM
Unidad de Investigación en Gerontología, FES Zaragoza, UNAM, Batalla 5 de Mayo s/n, esq. Fuerte de Loreto, Col. Ejército de Oriente, C.P. 09230, Mexico City, Mexico. mendovic@servidor.unam.mx
Sánchez-Rodríguez
M A
MA
Retana-Ugalde
R
R
Vargas-Guadarrama
L A
LA
Altamirano-Lozano
M A
MA
D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000097:blood / Q000235:genetics*; D000975:Antioxidants / Q000378:metabolism*; D004249:DNA Damage; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D012307:Risk Factors; D012737:Sex Factors
D000975:Antioxidants