Determination of moxifloxacin in growth media by high-performance liquid chromatography.
A direct injection high-performance liquid chromatographic method with column switching has been developed to determine moxifloxacin in Mueller-Hinton broth. A LiChrocart 4-4 pre-column filled with a LiChrospher 100 RP 18, 5 microm and a 150 x 4.6 mm I.D. column packed with a Supelcozil ABZ+ Plus were used and led to a retention time of 5.70 min. Fluorescence detection allowed one to reach a quantification limit of 0.05 microg/ml with a 100-microl sample size. The standard curves were linear from 0.05 to 3.2 microg/ml. Intra- and inter-day imprecisions within the linearity range were < or =4.76 and < or =5.75%, respectively. The mean relative errors for the same day and the day-to-day inaccuracies ranged from -2.93 to +4.50% and from -1.10 to +6.00%, respectively. The method was demonstrated to be useful for pharmacokinetic-pharmacodynamic studies of moxifloxacin in an in vitro model.
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Laboratoire de Pharmacocinétique et de Pharmacie Clinique, Faculté de Pharmacie, Université Victor Segalen Bordeaux 2, France. boubakar.ba@phcocin.u-bordeaux2.fr
Etienne
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Ducint
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Quentin
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Saux
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D000890:Anti-Infective Agents / Q000032:analysis* / Q000493:pharmacokinetics; D019540:Area Under Curve; D001372:Aza Compounds; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D003470:Culture Media / Q000737:chemistry*; D024841:Fluoroquinolones; D008962:Models, Theoretical; D000077266:Moxifloxacin; D011804:Quinolines; D015203:Reproducibility of Results
D000890:Anti-Infective Agents; D001372:Aza Compounds; D003470:Culture Media; D024841:Fluoroquinolones; D011804:Quinolines; D000077266:Moxifloxacin