Characteristics of a column suitable for capacity gradient chromatography with a borate eluent.
In capacity gradient elution, the gradient separation of ionic species is achieved by decreasing the ion-exchange capacity of a column during the course of the separation. Diol-type hydroxy groups on the resin surface form anionic complexes with borate as an eluting reagent. Thus, a chemically bonded anion-exchange column enriched with residual hydroxy groups allows the creation of a capacity gradient. An increase in the amount of the complex formed gradually brings about a decrease in the ion-exchange capacity of the column, and strongly bound analyte ions are eluted. We investigated the characteristics of a column suitable for this eluent system. The concentration of borate eluent required to remove the ion-exchange capacity depended inversely on the ratio of the residual hydroxy groups to functional groups. On a column in which this ratio was approximately 100, the ion-exchange capacity could easily be adjusted by using a low concentration of mannitol as a competing reagent. Use of this column led to very small baseline shifts during the borate-mannitol gradients, and to the simultaneous determination of anions with widely varying retention times.
Yamamoto
A
A
Toyama Institute of Health, 17-1 Nakataikoyama, Kosugi-machi, Toyama 939-0363, Japan.
Kodama
S
S
Matsunaga
A
A
Inoue
Y
Y
Aoyama
T
T
Kumagai
Y
Y
D001881:Borates; D002852:Chromatography, Ion Exchange; D005504:Food Analysis
D001881:Borates