Dimethylamiloride-induced differentiation of HL-60 cells.
OBJECTIVE
To study the effect of 5-(N,N-dimethyl) amiloride (DMA) on the proliferation and differentiation of HL-60 cells in vitro.


METHODS
MTT assay to test cytotoxicity; cell staining and NBT reduction to test cell differentiation.


RESULTS
DMA inhibited HL-60 cells growth in a concentration-dependent manner, and IC50 value for 96 h was 31.7 (95% confidence limits: 6.3-57.1) mumol.L-1. DMA also induced granulocytic differentiation in HL-60 cells. The percentage of differentiating cells increased from 6.5% to 70% after DMA 100 mumol.L-1 treatment for 3 d. The differentiating effect of DMA was distinguishable from amiloride, 5-(N-ethyl-N-isopropyl)amiloride (EIPA), and (5-(N-ethyl-N-isopropyl)amiloride (MIA). None among the amiloride, EIPA, and MIA were capable of triggering the differentiation of HL-60 cells.


CONCLUSIONS
DMA inhibited the proliferation of HL-60 cells and induced differentiation of HL-60 cells.
Li
Y Y
YY
Institute of Medical Biochemistry, Guangdong Medical College, Zhanjiang, Guangdong 524023, China. Guangdong Key Lab for Research & Development of Natural Medicines, Zhanjiang, Guangdong 524023, China. liyunyuan@163.net
Liang
N C
NC
Jiang
L
L
Wu
T
T
Lin
X Y
XY
D000584:Amiloride / Q000031:analogs & derivatives / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D018922:HL-60 Cells / Q000187:drug effects; D006801:Humans; D020128:Inhibitory Concentration 50
C039465:5-dimethylamiloride; D000584:Amiloride