title: Multiresidue determination of organophosphorus and organochlorine pesticides in human biological fluids by capillary gas chromatography.
abstract: Two multiresidue analytical methods for the simultaneous determination of organophosphorus and organochlorine pesticides in human urine and serum samples are described. The first approach is based on liquid-liquid microextraction with dichloromethane, and the second uses solid-phase extraction with C18. In both methods, the extracts are analyzed by capillary gas chromatography using nitrogen-phosphorus detection (NPD) and electron-capture detection (ECD). Limits of detection of the overall procedure of analysis are at the low ng mL(-1) level. Stability experiments have been performed with spiked urine and serum samples stored at 4 degrees C for 1 month. Finally, the solid-phase extraction procedure was applied to real-world samples. Quantification was performed by NPD or ECD, and peak identity was confirmed by use of mass-selective detection (MSD).
authors.affiliation: Experimental Sciences Dept., University Jaume I, Castellón, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Pitarch
authors.forename: F J
authors.initials: FJ
authors.lastname: López
authors.forename: R
authors.initials: R
authors.lastname: Serrano
authors.forename: F
authors.initials: F
authors.lastname: Hernández
mesh_terms: D002849:Chromatography, Gas / Q000379:methods*; D006801:Humans; D006843:Hydrocarbons, Chlorinated; D007306:Insecticides / Q000032:analysis* / Q000097:blood / Q000652:urine; D009943:Organophosphorus Compounds; D010573:Pesticide Residues / Q000032:analysis*; D012015:Reference Standards; D015203:Reproducibility of Results
chemical_list: D006843:Hydrocarbons, Chlorinated; D007306:Insecticides; D009943:Organophosphorus Compounds; D010573:Pesticide Residues