Insertion of polydeoxyadenosine-rich sequences into an intergenic region increases transcription in Chlamydomonas reinhardtii chloroplasts.
In this study, chloroplast transformation in Chlamydomonas reinhardtii was used to insert a tract of polydeoxyadenosine, which is known to influence DNA structure and transcription in other systems, between the 3' end of the atpB gene, encoding the beta-subunit of the chloroplast ATP synthase, and a downstream chimeric gene, aadA, encoding antibiotic resistance. Run-on transcription and RNA analyses revealed that in cells containing (dA)40 and (dAAAGGG)8, aadA was transcribed at a higher rate, and its RNA accumulated to a relatively high level. It is concluded that poly(dA/dT) can function in the chloroplast as a transcription enhancer element. Therefore, the insertion of poly(dA/dT) sequence into the intergenic region of a multicistronic transcription unit may modulate gene expression at the transcriptional level.
Lisitsky
I
I
Department of Biology, Technion-Israel Institute of Technology, Haifa 32000, Israel.
Rott
R
R
Schuster
G
G
D000818:Animals; D016825:Chlamydomonas reinhardtii / Q000201:enzymology / Q000235:genetics*; D002736:Chloroplasts / Q000235:genetics / Q000378:metabolism*; D021901:DNA, Intergenic; D005786:Gene Expression Regulation; D011061:Poly A / Q000235:genetics*; D011067:Poly dA-dT / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D006180:Proton-Translocating ATPases / Q000235:genetics*; D012323:RNA Processing, Post-Transcriptional; D012333:RNA, Messenger / Q000096:biosynthesis; D015183:Restriction Mapping; D014158:Transcription, Genetic
D021901:DNA, Intergenic; D012333:RNA, Messenger; D011061:Poly A; C015465:poly(dA); D011067:Poly dA-dT; D006180:Proton-Translocating ATPases