title: Candida albicans colonization of surface-sealed interim soft liners.
abstract: OBJECTIVE
  This in-vivo investigation evaluated the effect of 2 denture sealer agents on the microbial colonization of a newly placed soft interim denture liner during a period of 14 days.
  
  
  METHODS
  An interim soft denture liner (Coe-Soft; GC America, Alsip, IL) was coated with 2 different denture surface sealants (Palaseal [Heraeus Kulzer, Irvine, CA] and Mono-Poly [Plastodent, New York, NY]). Three rectangular wells of 1 cm wide x 2 cm long x 2 mm deep were placed in the intaglio of 10 maxillary complete dentures and filled with the soft liner material. The soft liner surface was treated with Palaseal (first well) and Mono-Poly (second well), and the unsealed (third well) was used as a control. These were exposed to the oral cavity for 14 days. The effect the sealant had in the prevention of Candidal colonization in vivo of the soft liner material was evaluated. Microbiological specimens were recovered from all samples and cultivated. Microbiological data from the control and 2-test samples in each denture were tabulated, and statistical analyses were performed.
  
  
  RESULTS
  This investigation showed clear differences (p <.001) between the sealed and unsealed soft liners. The sealed material showed significantly less colonization by yeast and bacteria. Intercomparison of the surface denture sealers, Palaseal versus Mono-Poly, showed no statistically significant differences (p < .005) in total yeast or bacterial colonization.
  
  
  CONCLUSIONS
  Coating of Coe-Soft denture liner with either Palaseal or Mono-Poly significantly decreased yeast and bacterial colonization. .
authors.affiliation: Advanced Education Program in Prosthodontics, Department of Restorative Dentistry, University of Maryland, Baltimore, MD, USA.
authors.forename: L
authors.initials: L
authors.lastname: Olan-Rodriguez
authors.forename: G E
authors.initials: GE
authors.lastname: Minah
authors.forename: C F
authors.initials: CF
authors.lastname: Driscoll
mesh_terms: D000328:Adult; D000368:Aged; D000704:Analysis of Variance; D002176:Candida albicans / Q000254:growth & development*; D015169:Colony Count, Microbial; D003764:Dental Materials / Q000737:chemistry*; D017220:Dentin-Bonding Agents / Q000737:chemistry; D003822:Denture Liners / Q000382:microbiology*; D003827:Denture, Complete, Upper / Q000382:microbiology*; D005260:Female; D006801:Humans; D007764:Lacquer; D008297:Male; D008689:Methacrylates / Q000737:chemistry; D008875:Middle Aged; D009055:Mouth / Q000382:microbiology; D012116:Resins, Plant; D013223:Statistics as Topic; D018709:Statistics, Nonparametric; D013499:Surface Properties; D013997:Time Factors; D015003:Yeasts / Q000254:growth & development
chemical_list: D003764:Dental Materials; D017220:Dentin-Bonding Agents; D008689:Methacrylates; C072648:Palaseal; D012116:Resins, Plant; C069457:Coe-Soft denture lining material; C023806:Copalite