Identification of human and mouse HIRA-interacting protein-5 (HIRIP5), two mammalian representatives in a family of phylogenetically conserved proteins with a role in the biogenesis of Fe/S proteins.
The human HIRA protein is encoded from a region of chromosome 22q that is critical for the DiGeorge syndrome and the velocardiofacial syndrome. We have previously reported that it directly interacts with core histones, with a novel histone-binding protein, HIRIP3, and during mouse embryogenesis, with the developmentally regulated homeodomain protein Pax3, suggesting a promoter-targeted function at the chromatin level. We here report on HIRA-interacting protein 5 (HIRIP5), a small acidic protein that interacted with HIRA in a double-hybrid screen performed in yeast and in in vitro protein interaction experiments. HIRIP5 has highly conserved homologs in both prokaryotes and eukaryotes, including the NFU1 gene product which has been implicated in iron metabolism in mitochondria of the yeast Saccharomyces cerevisiae. By radioactive in situ hybridization, the HIRIP5 gene was mapped to the 2p13-p15 chromosomal region, separate from any region previously associated with DiGeorge syndrome.
Lorain
S
S
Interactions Moléculaires et Cancer, CNRS UMR 1598, Institut Gustave-Roussy, Villejuif, France.
Lécluse
Y
Y
Scamps
C
C
Mattéi
M G
MG
Lipinski
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism*; D018797:Cell Cycle Proteins; D002874:Chromosome Mapping; D017124:Conserved Sequence; D018076:DNA, Complementary / Q000235:genetics; D006367:HeLa Cells; D056488:Histone Chaperones; D006801:Humans; D017403:In Situ Hybridization; D066298:In Vitro Techniques; D007506:Iron-Sulfur Proteins / Q000096:biosynthesis*; D051379:Mice; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D018076:DNA, Complementary; C086057:HIRA protein, human; C484187:Hira protein, mouse; C501993:Hirip5 protein, mouse; D056488:Histone Chaperones; D007506:Iron-Sulfur Proteins; C426067:NFU1 protein, human; D009687:Nuclear Proteins; D014157:Transcription Factors