Phosphorylation and metabolism of sucrose and its five linkage-isomeric alpha-D-glucosyl-D-fructoses by Klebsiella pneumoniae.
Not only sucrose but the five isomeric alpha-D-glucosyl-D-fructoses trehalulose, turanose, maltulose, leucrose, and palatinose are utilized by Klebsiella pneumoniae as energy sources for growth, thereby undergoing phosphorylation by a phosphoenolpyruvate-dependent phosphotransferase system uniformly at 0-6 of the glucosyl moiety. Similarly, maltose, isomaltose, and maltitol, when exposed to these conditions, are phosphorylated regiospecifically at O-6 of their non-reducing glucose portion. The structures of these novel compounds have been established unequivocally by enzymatic analysis, acid hydrolysis, FAB negative-ion spectrometry, and 1H and 13C NMR spectroscopy. In cells of K. pneumoniae, hydrolysis of sucrose 6-phosphate is catalyzed by sucrose 6-phosphate hydrolase from Family 32 of the glycosylhydrolase superfamily. The five 6'-O-phosphorylated alpha-D-glucosyl-fructoses are hydrolyzed by an inducible (approximately 49-50 Kda) phospho-alpha-glucosidase from Family 4 of the glycosylhydrolase superfamily.
Thompson
J
J
Oral Infection and Immunity Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892-4350, USA. jthompson@dir.nidcr.nih.gov
Robrish
S A
SA
Pikis
A
A
Brust
A
A
Lichtenthaler
F W
FW
D000595:Amino Acid Sequence; D002236:Carbohydrate Conformation; D002855:Chromatography, Thin Layer; D004187:Disaccharides / Q000378:metabolism*; D005632:Fructose / Q000737:chemistry*; D006026:Glycoside Hydrolases / Q000378:metabolism; D006868:Hydrolysis; D015151:Immunoblotting; D007534:Isomaltose / Q000031:analogs & derivatives* / Q000378:metabolism; D007536:Isomerism; D007711:Klebsiella pneumoniae / Q000201:enzymology / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D015394:Molecular Structure; D010766:Phosphorylation; D013395:Sucrose / Q000737:chemistry / Q000378:metabolism*; D000520:alpha-Glucosidases / Q000737:chemistry / Q000378:metabolism; D043324:beta-Fructofuranosidase
D004187:Disaccharides; D005632:Fructose; D013395:Sucrose; D007534:Isomaltose; C029454:turanose; D006026:Glycoside Hydrolases; C049150:maltose-6'-phosphate glucosidase; D000520:alpha-Glucosidases; D043324:beta-Fructofuranosidase; C042003:trehalulose; C008189:isomaltulose; C060525:leucrose