Human placental calreticulin characterization of domain structure and post-translational modifications.
The domain organization and the post-translational modifications of human placenta calreticulin were analysed by MS in combination with proteolytic digestion. Prolonged treatment with trypsin, chymotrypsin, elastase, Staphylococcus aureus V8 protease, or proteinase K all led to a 6- to 7-kDa decrease in the molecular mass of calreticulin. The decrease was found to be due to cleavages in the region around residue 340. In addition, minor fragments resulting from secondary cleavages close to the N-terminus were observed, but no stable fragments of intermediate size were found. These results show that the C-domain of calreticulin is susceptible to proteolytic cleavage and that the N- and P-domains form a proteolytically stable tight association. A disulfide bridge between the first two cysteines was mapped in the N-domain, and the third cysteine was found in the reduced form. No post-translational modifications in the form of glycosylation or phosphorylation were found. A modified form of calreticulin lacking the C-terminal hexapeptide including the KDEL endoplasmic reticulum retention sequon was isolated. Such a truncation may point to a mechanism that allows escape of calreticulin from the endoplasmic reticulum.
Højrup
P
P
Institute of Biochemistry and Molecular Biology, Odense University, University of Southern Denmark, Odense, Denmark. php@bmb.sdu.dk
Roepstorff
P
P
Houen
G
G
D000595:Amino Acid Sequence; D002135:Calcium-Binding Proteins / Q000737:chemistry* / Q000378:metabolism*; D037282:Calreticulin; D004721:Endoplasmic Reticulum / Q000378:metabolism; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D011499:Protein Processing, Post-Translational; D017434:Protein Structure, Tertiary; D012261:Ribonucleoproteins / Q000737:chemistry* / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D002135:Calcium-Binding Proteins; D037282:Calreticulin; D010446:Peptide Fragments; D012261:Ribonucleoproteins