title: Ion interaction reagent reversed-phase high-performance liquid chromatography determination of anti-tuberculosis drugs and metabolites in biological fluids.
abstract: New methods of ion interaction reagent (IIR) RP-HPLC are presented for the determination of anti-tuberculosis drugs and their metabolites, singly or in multi-component mixtures, in biological fluids. The following analytes are considered: isoniazid, ethionamide, pyrazinamide, morphazinamide, p-aminosalicylic acid, nicotinic and isonicotinic acids. Aqueous solutions of three different ion interaction reagents are alternatively or comparatively used as the mobile phases, namely: (A) 5.00 mM octylamine at pH 3.00 for o-phosphoric acid, (B) 5.00 mM octylamine at pH 8.00 for o-phosphoric acid, and (C) 5.00 mM 1,6 diaminohexane at pH 6.00 for o-phosphoric acid. The response linearity between peak area and analyte concentration is verified for all the analytes in the concentration range within the determination limits and 2.00 mg/l. Detection limits are always lower than 82 microg/l for standard solutions; in the analysis of samples of rat serum, rat plasma and human serum, the matrix effect is negligible, the detection limits are always lower than 94 microg/l and the average recovery yield is always greater than 96%.
authors.affiliation: Dipartimento di Scienze e Tecnologie Avanzate, Università del Piedmonte Orientale Amadeo Avogadro, Alessandria, Italy. gennaro@mfn.unipmn.it
authors.forename: M C
authors.initials: MC
authors.lastname: Gennaro
authors.forename: R
authors.initials: R
authors.lastname: Calvino
authors.forename: C
authors.initials: C
authors.lastname: Abrigo
mesh_terms: D010131:Aminosalicylic Acid / Q000097:blood / Q000378:metabolism; D000818:Animals; D000995:Antitubercular Agents / Q000097:blood* / Q000378:metabolism; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005000:Ethionamide / Q000097:blood / Q000378:metabolism; D006801:Humans; D007538:Isoniazid / Q000097:blood / Q000378:metabolism; D007539:Isonicotinic Acids / Q000302:isolation & purification; D009525:Niacin / Q000302:isolation & purification; D011718:Pyrazinamide / Q000097:blood / Q000378:metabolism; D011719:Pyrazines / Q000097:blood / Q000378:metabolism; D011786:Quality Control; D051381:Rats
chemical_list: D000995:Antitubercular Agents; D007539:Isonicotinic Acids; D011719:Pyrazines; D009525:Niacin; D011718:Pyrazinamide; D010131:Aminosalicylic Acid; C038993:morphazinamide; D005000:Ethionamide; D007538:Isoniazid