title: Accurate mass measurement of synthetic analogues of prazosine by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry.
abstract: Matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOFMS) has been investigated as a tool for accurate determination of the molecular mass of synthetic analogues of prazosine, a molecule used for the treatment of hypertension. Samples were dissolved in methanol, mixed with mass calibration standards, and crystallised on the target with alpha-cyano-4-hydroxycinnamic acid as matrix. Acquisition of spectra was rapidly completed in reflectron mode, allowing high resolution (6000-10000) and sensitive (about 1-10 pmol of sample on target) determination of the synthetic products. The results show the effectiveness of MALDI-TOFMS for accurate mass determination of these fairly large molecules, which are otherwise difficult to analyse by other high-resolution mass spectrometric techniques.
authors.affiliation: Department of Chemistry 'G. Ciamician', University of Bologna, Italy. andalo@ciam.unibo.it
authors.forename: C
authors.initials: C
authors.lastname: Andalò
authors.forename: P
authors.initials: P
authors.lastname: Bocchini
authors.forename: R
authors.initials: R
authors.lastname: Pozzi
authors.forename: G C
authors.initials: GC
authors.lastname: Galletti
mesh_terms: D000317:Adrenergic alpha-Antagonists / Q000032:analysis*; D002138:Calibration; D011224:Prazosin / Q000031:analogs & derivatives* / Q000032:analysis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000317:Adrenergic alpha-Antagonists; D011224:Prazosin