title: Prolonged immobilization-induced stress delays alveolar bone healing. A histometric study in rats.
abstract: The purpose of the present study was to investigate the effect of prolonged immobilization-induced stress on reparative bone formation, using the rat alveolar healing as an experimental protocol. Stress was attained by immobilization for 2 hours a day, beginning three days before extraction of the upper right incisors and continuing until sacrifice. The stress condition was assayed on the basis of plasma corticosterone concentration (measured by double-antibody radioimmunoassay), which increased by 2.5 to 4 times in rats submitted to immobilization. The volume density of neoformed bone filling the alveolar socket was quantified by a histometric differential point-counting method 7 to 21 days following tooth extraction. Stress caused a significant delay in reparative bone increment, somewhat related to impairment of coagulum remission and/or organization.
authors.affiliation: Department of Morphology, Stomatology and Physiology, Dental School of Ribeirão Preto, University of São Paulo, Brasil.
authors.forename: K F
authors.initials: KF
authors.lastname: Prado
authors.forename: T L
authors.initials: TL
authors.lastname: Carvalho
authors.forename: J A
authors.initials: JA
authors.lastname: Franci
authors.forename: L G
authors.initials: LG
authors.lastname: Brentegani
mesh_terms: D000818:Animals; D001861:Bone Regeneration; D003345:Corticosterone / Q000032:analysis / Q000097:blood; D007103:Immobilization / Q000009:adverse effects*; D008297:Male; D016555:Matched-Pair Analysis; D011863:Radioimmunoassay; D051381:Rats; D017208:Rats, Wistar; D013312:Stress, Physiological; D013997:Time Factors; D020390:Tooth Socket / Q000502:physiology*; D014945:Wound Healing
chemical_list: D003345:Corticosterone