Toxicology of Kalopanax pictus extract and hematological effect of the isolated anti-rheumatoidal kalopanaxsaponin A on the Freunds complete adjuvant reagent-treated rat.
We have reported that kalopanaxsaponin A (KPS-A) isolated from Kalopanax pictus have anti-rheumatoidal activity in the rat treated with Freunds complete adjuvant (FCA) reagent. In addition, it has been also reported that KPS-A is a potent antioxidant in the rheumatoidal rat. This research was undertaken to examine whether the saponins of KPS-A and -I could adjust the abnormal lipid metabolisms and hematological changes in immunological diseases. KPS-A significantly inhibited the increases in both triglycerides and total proteins in addition to the decrease in total cholesterol induced by FCA reagent treatment. KPS-A treatment decreased the number of leucocytes elevated by FCA reagent treatment. Excess dose of the methanol extract produced no severe toxicity on the body weight, wet organ weights and hepatic functions. Since LD50 value of K. pictus methanol extract was shown to be 4,033 mg/kg, it could be estimated to be a safe agent for anti-rheumatoidal herbal medicines.
Choi
J
J
College of Pharmacy, Kyungsung University, Pusan, Korea.
Huh
K
K
Kim
S H
SH
Lee
K T
KT
Kwon
S H
SH
Park
H J
HJ
D000818:Animals; D018501:Antirheumatic Agents / Q000494:pharmacology* / Q000633:toxicity*; D001169:Arthritis, Experimental / Q000188:drug therapy*; D001772:Blood Cell Count; D001835:Body Weight / Q000187:drug effects; D002784:Cholesterol / Q000097:blood; D004365:Drugs, Chinese Herbal / Q000494:pharmacology* / Q000633:toxicity*; D005620:Freund's Adjuvant; D007202:Indicators and Reagents; D007770:L-Lactate Dehydrogenase / Q000097:blood; D007928:Lethal Dose 50; D008111:Liver Function Tests; D051379:Mice; D008813:Mice, Inbred ICR; D009828:Oleanolic Acid / Q000031:analogs & derivatives*; D009929:Organ Size / Q000187:drug effects; D010936:Plant Extracts / Q000633:toxicity; D010946:Plants, Medicinal / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012503:Saponins / Q000494:pharmacology* / Q000633:toxicity*; D014280:Triglycerides / Q000097:blood
D018501:Antirheumatic Agents; D004365:Drugs, Chinese Herbal; D007202:Indicators and Reagents; D010936:Plant Extracts; D012503:Saponins; D014280:Triglycerides; C010177:beta-hederin; D009828:Oleanolic Acid; D005620:Freund's Adjuvant; D002784:Cholesterol; D007770:L-Lactate Dehydrogenase