title: Rapid detection of chromosome aneuploidies by prenatal interphase FISH (fluorescence in situ hybridization) and its clinical utility in Japan.
abstract: OBJECTIVE
  The purpose of this study was to assess the accuracy, informative rate, detection rate, and clinical utility of prenatal interphase fluorescence in situ hybridization (FISH) analysis of amniotic fluid samples from Japanese women.
  
  
  METHODS
  Amniotic fluid specimens from 2,639 Japanese women were received for prenatal interphase FISH and chromosome analysis. A questionnaire was designed to evaluate FISH clinical utility by collaboration sites.
  
  
  RESULTS
  Based on 2,319 tested samples, the accuracy (100%), informative (94%), and detection (87.6%) rates were all high. The accuracy (100%), informative (90.2%), and detection (90.0%) rates were also remarkable in third-trimester pregnancies. We perceive significant advantages from this test regarding medical management and patient satisfaction.
  
  
  CONCLUSIONS
  This novel report shows that in Japan prenatal interphase FISH testing is highly informative and accurate, not only in second-trimester pregnancies but also in third-trimester pregnancies. This test provides advantages to both physicians and patients, provided that its capabilities and limitations are understood.
authors.affiliation: Department of Obstetrics and Gynecology, Nippon Medical School, Tokyo, Japan.
authors.forename: R
authors.initials: R
authors.lastname: Sawa
authors.forename: Z
authors.initials: Z
authors.lastname: Hayashi
authors.forename: T
authors.initials: T
authors.lastname: Tanaka
authors.forename: T
authors.initials: T
authors.lastname: Onda
authors.forename: K
authors.initials: K
authors.lastname: Hoshi
authors.forename: Y
authors.initials: Y
authors.lastname: Fukada
authors.forename: Y
authors.initials: Y
authors.lastname: Takai
authors.forename: Y
authors.initials: Y
authors.lastname: Taketani
authors.forename: T
authors.initials: T
authors.lastname: Kubo
authors.forename: H
authors.initials: H
authors.lastname: Hamada
authors.forename: K
authors.initials: K
authors.lastname: Yoshida
authors.forename: Y
authors.initials: Y
authors.lastname: Nakamura
authors.forename: T
authors.initials: T
authors.lastname: Okai
authors.forename: M
authors.initials: M
authors.lastname: Sakai
authors.forename: T
authors.initials: T
authors.lastname: Kaneoka
authors.forename: Y
authors.initials: Y
authors.lastname: Makino
authors.forename: T
authors.initials: T
authors.lastname: Aono
authors.forename: K
authors.initials: K
authors.lastname: Maeda
authors.forename: R
authors.initials: R
authors.lastname: Honda
authors.forename: H
authors.initials: H
authors.lastname: Okamura
authors.forename: H
authors.initials: H
authors.lastname: Sago
authors.forename: M
authors.initials: M
authors.lastname: Kitagawa
authors.forename: S
authors.initials: S
authors.lastname: Minoura
authors.forename: J
authors.initials: J
authors.lastname: Inaba
authors.forename: N
authors.initials: N
authors.lastname: Terakawa
authors.forename: N
authors.initials: N
authors.lastname: Nagata
authors.forename: K
authors.initials: K
authors.lastname: Shimomura
authors.forename: M J
authors.initials: MJ
authors.lastname: Sapeta
authors.forename: L L
authors.initials: LL
authors.lastname: Estabrooks
mesh_terms: D000328:Adult; D000653:Amniotic Fluid; D002869:Chromosome Aberrations / Q000175:diagnosis*; D025063:Chromosome Disorders; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D017060:Patient Satisfaction; D011237:Predictive Value of Tests; D011247:Pregnancy; D011262:Pregnancy Trimester, Second; D011263:Pregnancy Trimester, Third; D011296:Prenatal Diagnosis; D011795:Surveys and Questionnaires