No evidence for deletions of the NBS1 gene in lymphomas.
Patients with Nijmegen breakage syndrome (NBS) have a high risk to develop malignant diseases, most frequently B-cell lymphomas. The NBS gene product, nibrin, is involved in DNA recombination repair, a function shared with known tumor suppressor genes like BRCA1 and BRCA2. This led us to investigate whether NBS acts as tumor suppressor gene in the development of non-Hodgkin lymphomas. Therefore, we performed fluorescence in situ hybridization analysis using a BAC clone containing the entire NBS1 region on eight B-cell and eight T-cell lymphomas, including one B-cell and two T-cell lymphomas with structural abnormalities of 8q. None of the tumors showed a deletion of the NBS1 gene, demonstrating that deletion of the NBS1 gene is not a major cause or a primary event in tumorigenesis of human B- and T-cell lymphomas.
Stumm
M
M
Department of Human Genetics, Otto-von-Guericke University, Leipziger Strasse 44, 39120, Magdeburg, Germany. markus.stumm@medizin.uni-magdeburg.de
von Ruskowsky
A
A
Siebert
R
R
Harder
S
S
Varon
R
R
Wieacker
P
P
Schlegelberger
B
B
D017353:Gene Deletion; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D016393:Lymphoma, B-Cell / Q000235:genetics*; D016399:Lymphoma, T-Cell / Q000235:genetics*; D009687:Nuclear Proteins / Q000235:genetics*
D009687:Nuclear Proteins