Helicobacter pylori infection in the cat: evaluation of gastric colonization, inflammation and function.
BACKGROUND
Further elucidation of the consequences of Helicobacter pylori infection on gastric mucosal inflammation and gastric secretory function would be facilitated by an animal model that is susceptible to infection with H. pylori, is broadly similar in gastric physiology and pathology to people, and is amenable to repeated non-invasive evaluation. The goal of this study was to examine the interrelationship of bacterial colonization, mucosal inflammation and gastric secretory function in cats with naturally acquired H. pylori infection.


METHODS
Twenty clinically healthy cats with naturally acquired H. pylori infection (cagA-, picB) and 19 Helicobacter-free cats were evaluated. Gastric colonization was determined by tissue urease activity, light microscopy, culture and PCR. The mucosal inflammatory response was evaluated by light microscopy, and by RT-PCR of the pro-inflammatory cytokines IL-1alpha, IL-1beta, IL-8 and TNF-alpha in gastric mucosa. Gastric secretory function was assessed by measuring pentagastrin-stimulated acid secretion, fasting plasma gastrin, and antral mucosal gastrin and somatostatin immunoreactivity.


RESULTS
H. pylori colonized the pylorus, fundus and cardia in similar density. Bacteria were observed free in the lumen of gastric glands and were also tightly adherent to epithelial cells where they were associated with microvillus effacement. Mononuclear inflammation, lymphoid follicle hyperplasia, atrophy and fibrosis were observed primarily in H. pylori-infected cats, with the pylorus most severely affected. Neutrophilic and eosinophilic infiltrates, epithelial dysplasia, and up-regulation of mucosal IL-1beta and IL-8 were observed solely in infected cats. Fasting plasma gastrin concentrations and pentagastrin-stimulated acid output were similar in both infected and uninfected cats. There was no relationship of bacterial colonization density or gastric inflammation to plasma gastrin concentrations or gastric acid output.


CONCLUSIONS
The pattern of colonization and the mucosal inflammatory response in cats with naturally acquired H. pylori are broadly similar to those in infected people, particularly children, and non-human primates. The upregulation of IL-8 in infected cats was independent of cagA and picB. Our findings argue against a direct acid-suppressing effect of H. pylori on the gastric secretory-axis in chronically infected cats.
Simpson
K W
KW
College of Veterinary Medicine, Cornell University, Ithaca NY 14853, USA.
Strauss-Ayali
D
D
Straubinger
R K
RK
Scanziani
E
E
McDonough
P L
PL
Straubinger
A F
AF
Chang
Y F
YF
Esteves
M I
MI
Fox
J G
JG
Domeneghini
C
C
Arebi
N
N
Calam
J
J
D000818:Animals; D000942:Antigens, Bacterial; D001426:Bacterial Proteins / Q000378:metabolism; D002299:Cardia / Q000382:microbiology / Q000473:pathology; D002371:Cat Diseases / Q000378:metabolism / Q000382:microbiology* / Q000473:pathology; D002415:Cats; D004195:Disease Models, Animal; D005260:Female; D005745:Gastric Acidity Determination; D005748:Gastric Fundus / Q000382:microbiology / Q000473:pathology; D005753:Gastric Mucosa / Q000378:metabolism / Q000382:microbiology*; D005755:Gastrins / Q000378:metabolism; D005756:Gastritis / Q000378:metabolism / Q000382:microbiology / Q000662:veterinary*; D016481:Helicobacter Infections / Q000378:metabolism / Q000473:pathology / Q000662:veterinary*; D016480:Helicobacter pylori; D007375:Interleukin-1 / Q000096:biosynthesis; D016209:Interleukin-8 / Q000096:biosynthesis; D008297:Male; D011706:Pyloric Antrum / Q000378:metabolism / Q000382:microbiology / Q000473:pathology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013004:Somatostatin / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D005755:Gastrins; D007375:Interleukin-1; D016209:Interleukin-8; C103937:PicB protein, Helicobacter pylori; D014409:Tumor Necrosis Factor-alpha; C080866:cagA protein, Helicobacter pylori; D013004:Somatostatin