Involvement of the cytoskeleton in regulation of collagen synthesis and secretion.
11-day chick embryo skin fibroblasts administered in vitro with colchicine or cytochalasin B, have been investigated for collagen synthesis and secretion. The cytoskeleton, examined by confocal laser scanning immunofluorescence microscope, exibited in colchicine treated cells a clear loss of microtubules, in cytochalasin B treated cells a diffuse staining and patches of intracellular fluorescence caused by actin disassembly. Cytochalasin B treatment decreased endocellular type I collagen synthesis and the total uptake of 3H-proline, while enhanced collagen secretion. Colchicine did not interfere with the collagen secretion and the label uptake, whereas it increased endocellular collagen synthesis. In addition, both drugs induced an increase of the alpha2:alpha1 ratio of collagen chains. The results obtained suggest that the cytoskeleton controls not only the morphological organization of cell components, but also the organization of biochemical processes.
Carinci
P
P
Istituto di Istologia ed Embriologia Generale-Università di Ferrara, Italia.
Bodo
M
M
Evangelisti
R
R
Becchetti
E
E
D008841:Actin Cytoskeleton / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D000199:Actins / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D000818:Animals; D002478:Cells, Cultured / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D002642:Chick Embryo; D003078:Colchicine / Q000494:pharmacology; D003094:Collagen / Q000096:biosynthesis* / Q000187:drug effects / Q000378:metabolism*; D003571:Cytochalasin B / Q000494:pharmacology; D003599:Cytoskeleton / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006074:Gout Suppressants / Q000494:pharmacology; D008870:Microtubules / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D011392:Proline / Q000493:pharmacokinetics; D014316:Tritium / Q000493:pharmacokinetics
D000199:Actins; D006074:Gout Suppressants; D014316:Tritium; D003571:Cytochalasin B; D003094:Collagen; D011392:Proline; D003078:Colchicine