Whole-mount in situ hybridization to mouse embryos.
The mouse is well-established as the major animal model for the study of mammalian development. Rapid progress in large-scale cDNA and also genomic sequencing projects is identifying new mouse genes at an unprecedented rate. As a first step toward understanding the function of these novel genes, it is important to determine their developmental expression pattern. Here we provide a reliable, sensitive method for whole-mount in situ hybridization using the mouse embryo.
Correia
K M
KM
Department of Genetics, Case Western Reserve University, Cleveland, Ohio 44106-4955, USA. kmc@stowers-institute.org
Conlon
R A
RA
D000818:Animals; D018076:DNA, Complementary / Q000378:metabolism; D004622:Embryo, Mammalian / Q000378:metabolism*; D019286:Endopeptidase K / Q000378:metabolism; D005821:Genetic Techniques; D017403:In Situ Hybridization / Q000379:methods*; D051379:Mice
D018076:DNA, Complementary; D019286:Endopeptidase K