An aminoacyl tRNA synthetase whose sequence fits into neither of the two known classes.
Aminoacyl transfer RNA synthetases catalyse the first step of protein synthesis and establish the rules of the genetic code through the aminoacylation of tRNAs. There is a distinct synthetase for each of the 20 amino acids and throughout evolution these enzymes have been divided into two classes of ten enzymes each. These classes are defined by the distinct architectures of their active sites, which are associated with specific and universal sequence motifs. Because the synthesis of aminoacyl-tRNAs containing each of the twenty amino acids is a universally conserved, essential reaction, the absence of a recognizable gene for cysteinyl tRNA synthetase in the genomes of Archae such as Methanococcus jannaschii and Methanobacterium thermoautotrophicum has been difficult to interpret. Here we describe a different cysteinyl-tRNA synthetase from M. jannaschii and Deinococcus radiodurans and its characterization in vitro and in vivo. This protein lacks the characteristic sequence motifs seen in the more than 700 known members of the two canonical classes of tRNA synthetase and may be of ancient origin. The existence of this protein contrasts with proposals that aminoacylation with cysteine in M. jannaschii is an auxiliary function of a canonical prolyl-tRNA synthetase.
Fàbrega
C
C
The Skaggs Institute for Chemical Biology, The Scripps Research Institute, Beckman Center, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.
Farrow
M A
MA
Mukhopadhyay
B
B
de Crécy-Lagard
V
V
Ortiz
A R
AR
Schimmel
P
P
D000595:Amino Acid Sequence; D000604:Amino Acyl-tRNA Synthetases / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000378:metabolism; D019843:Archaeal Proteins; D003001:Cloning, Molecular; D003545:Cysteine / Q000378:metabolism; D004926:Escherichia coli; D016680:Genome, Bacterial; D017014:Methanobacterium / Q000201:enzymology*; D017017:Methanococcus / Q000201:enzymology*; D008969:Molecular Sequence Data; D016415:Sequence Alignment
D019843:Archaeal Proteins; D000604:Amino Acyl-tRNA Synthetases; C428084:mj1477 protein, Euryarchaeota; C019975:cysteinyl-tRNA synthetase; D003545:Cysteine