title: Roles of the hinge region and the DNA binding domain of the bovine papillomavirus type 1 E2 protein in initiation of DNA replication.
abstract: The bovine papillomavirus (BPV-1) E2 protein is the regulator of extrachromosomal replication of papillomaviruses. The mutants with C-terminal truncations and in-frame internal deletions were constructed to study the role of structural domains of E2 in the initiation of DNA replication. We show that the replication initiation function of E2 is absolutely dependent on the ability of the protein to bind to DNA. Our study also confirms the borders of the functional domains of the E2 protein; residues 1-192 form the activation domain and residues 310-410 the DNA binding-dimerization domain. Some critical length and flexibility, but not the particular amino acid sequence between these two functional domains is required for the activity of the protein to support replication. The hinge region, including the major phosphorylation sites of E2, is also dispensable for the mediation of attachment of the BPV1 genome to the mitotic chromosomes.
authors.affiliation: Department of Microbiology and Virology, Institute of Molecular and Cell Biology, Tartu University and Estonian Biocentre, 23 Riia Street, 51010, Tartu, Estonia.
authors.forename: A
authors.initials: A
authors.lastname: Allikas
authors.forename: D
authors.initials: D
authors.lastname: Ord
authors.forename: R
authors.initials: R
authors.lastname: Kurg
authors.forename: S
authors.initials: S
authors.lastname: Kivi
authors.forename: M
authors.initials: M
authors.lastname: Ustav
mesh_terms: D000818:Animals; D001665:Binding Sites / Q000235:genetics; D052688:Bovine papillomavirus 1 / Q000235:genetics / Q000502:physiology*; D016466:CHO Cells; D019556:COS Cells; D002417:Cattle; D002460:Cell Line; D006224:Cricetinae; D004261:DNA Replication / Q000235:genetics; D004279:DNA, Viral / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D010446:Peptide Fragments / Q000502:physiology*; D010766:Phosphorylation; D010957:Plasmids / Q000138:chemical synthesis; D017434:Protein Structure, Tertiary / Q000235:genetics; D017384:Sequence Deletion; D014764:Viral Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D014779:Virus Replication / Q000235:genetics
chemical_list: D004279:DNA, Viral; D004268:DNA-Binding Proteins; C051550:E2 protein, Bovine papillomavirus; D010446:Peptide Fragments; D014764:Viral Proteins