title: The hemagglutinin-neuraminidase protein of peste des petits ruminants virus is biologically active when transiently expressed in mammalian cells.
abstract: The genes coding for the surface glycoproteins hemagglutinin-neuraminidase (HN) of the peste des petits ruminants virus (PPRV) and hemagglutinin (H) of rinderpest virus (RPV) were cloned in a cytomagalovirus promoter driven expression vector and expressed transiently in mammalian cells. The protein expression was apparent 24 h after transfection and the expressed proteins were detected at the cell surface. The transiently expressed PPRV HN protein was found to be biologically active in possessing hemadsorption and neuraminidase activities. On the other hand, RPV H protein exhibited neuraminidase activity but was deficient in hemadsorption activity. The substrate specificity of the neuraminidase activity of these two proteins differed distinctly. The presence of neuraminidase activity in both PPRV HN and RPV H proteins is unusual among members of the morbillivirus genus.
authors.affiliation: Department of Microbiology and Cell Biology, Indian Institute of Science, 560012, Bangalore, India.
authors.forename: S
authors.initials: S
authors.lastname: Seth
authors.forename: M S
authors.initials: MS
authors.lastname: Shaila
mesh_terms: D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D003587:Cytomegalovirus / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics; D015967:Gene Expression Regulation, Viral / Q000235:genetics; D005814:Genes, Viral / Q000235:genetics; D015707:HN Protein / Q000096:biosynthesis* / Q000235:genetics*; D006382:Hemadsorption / Q000235:genetics; D006801:Humans; D009439:Neuraminidase / Q000235:genetics; D018107:Peste-des-petits-ruminants virus / Q000201:enzymology / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis; D014162:Transfection; D014709:Vero Cells; D015678:Viral Structural Proteins / Q000235:genetics
chemical_list: D015707:HN Protein; D011994:Recombinant Proteins; D015678:Viral Structural Proteins; D009439:Neuraminidase