title: Antibodies against a putative heparin receptor slow cell proliferation and decrease MAPK activation in vascular smooth muscle cells.
abstract: Heparin has long been known to slow the growth of vascular smooth muscle cells. However, the mechanism(s) by which heparin acts has yet to be resolved. The identification of a putative heparin receptor in endothelial cells with antibodies that blocked heparin binding to the cells provided the means to further examine the possible involvement of a heparin receptor in smooth muscle cell responses to heparin. Immunoprecipitation of a smooth muscle cell protein with the anti-heparin receptor antibodies provided evidence that the protein was present in smooth muscle cells. Experiments with the anti-heparin receptor antibodies indicate that the antibodies can mimic heparin in decreasing PDGF induced thymidine and BrdU incorporation. The anti-heparin receptor antibodies were also found to decrease MAPK activity levels after activation similarly to heparin. These results support the identification of a heparin receptor and its role in heparin effects on vascular smooth muscle cell growth.
authors.affiliation: Department of Biological Sciences, Lehigh University, Bethlehem, Pennsylvania 18015, USA.
authors.forename: J M
authors.initials: JM
authors.lastname: Savage
authors.forename: A C
authors.initials: AC
authors.lastname: Gilotti
authors.forename: C A
authors.initials: CA
authors.lastname: Granzow
authors.forename: F
authors.initials: F
authors.lastname: Molina
authors.forename: L J
authors.initials: LJ
authors.lastname: Lowe-Krentz
mesh_terms: D000818:Animals; D000906:Antibodies / Q000494:pharmacology*; D001011:Aorta; D015153:Blotting, Western; D001973:Bromodeoxyuridine; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D004789:Enzyme Activation / Q000187:drug effects; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000378:metabolism*; D010982:Platelet-Derived Growth Factor / Q000037:antagonists & inhibitors / Q000494:pharmacology; D011956:Receptors, Cell Surface / Q000037:antagonists & inhibitors* / Q000378:metabolism; D013552:Swine; D013936:Thymidine / Q000378:metabolism
chemical_list: D000906:Antibodies; D010982:Platelet-Derived Growth Factor; D011956:Receptors, Cell Surface; C037680:heparin receptor; D004247:DNA; D020928:Mitogen-Activated Protein Kinases; D001973:Bromodeoxyuridine; D013936:Thymidine