[Regulation of ACTH4-9 analogue on the Ca2+ level of mouse hippocampal synaptosomes].
The effect of ACTH4-9 analogue Org2766 on the [Ca2+]i level of mouse hippocampal synaptosomes was studied. Synaptosomal free calcium concentration ([Ca2+]i) and 45Ca2+ uptake were measured respectively by spex cation measurement system and Beckman LS-9800 liquid scintillation spectrometer. Low dose of Org2766 did not change synaptosomal [Ca2+]i level, but decreased 45Ca2+ uptake. High dose of Org2766 increased [Ca2+]i without affecting 45Ca2+ uptake. Low dose of Org2766 inhibited the anisomysin-induced increase of synaptosomal [Ca2+]i while high dose had no effect. These results suggest that the regulation of Org2766 on hippocampal synaptosomal Ca2+ level may be achieved by inhibiting the Ca2+ influx and increasing the release of synaptosomal calcium pool.
Luo
L
L
Department of Biological Science and Technology, Nanjing University, Nanjing 210093.
Zhang
W N
WN
Zheng
Y L
YL
Wu
F M
FM
D000324:Adrenocorticotropic Hormone / Q000031:analogs & derivatives* / Q000494:pharmacology*; D000818:Animals; D001693:Biological Transport, Active; D002118:Calcium / Q000378:metabolism*; D002132:Calcium Radioisotopes / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism*; D066298:In Vitro Techniques; D051379:Mice; D010446:Peptide Fragments / Q000494:pharmacology*; D013574:Synaptosomes / Q000378:metabolism*
D002132:Calcium Radioisotopes; D010446:Peptide Fragments; C013765:Org 2766; C028024:ACTH (4-9); D000324:Adrenocorticotropic Hormone; D002118:Calcium