title: Intracellular persistence of Staphylococcus aureus small-colony variants within keratinocytes: a cause for antibiotic treatment failure in a patient with darier's disease.
abstract: Intracellular persistence assays were performed with small-colony variants (SCVs) derived from a patient with Darier's disease from whom different phenotypes and genotypes of Staphylococcus aureus were isolated over a 28-month period; the assays revealed that >100-fold more SCV cells persisted intracellularly relative to the normal phenotype. The presence of intracellular S. aureus SCVs may protect against host defenses and antibiotic therapy and thus may have contributed to this patient's very prolonged skin infection.
authors.affiliation: Institute of Medical Microbiology, Westfälische Wilhelms-Universität Münster, Münster, Germany. eiffc@uni-muenster.de
authors.forename: C
authors.initials: C
authors.lastname: von Eiff
authors.forename: K
authors.initials: K
authors.lastname: Becker
authors.forename: D
authors.initials: D
authors.lastname: Metze
authors.forename: G
authors.initials: G
authors.lastname: Lubritz
authors.forename: J
authors.initials: J
authors.lastname: Hockmann
authors.forename: T
authors.initials: T
authors.lastname: Schwarz
authors.forename: G
authors.initials: G
authors.lastname: Peters
mesh_terms: D004269:DNA, Bacterial / Q000032:analysis; D007644:Darier Disease / Q000188:drug therapy / Q000382:microbiology*; D006801:Humans; D007424:Intracellular Fluid / Q000382:microbiology; D015603:Keratinocytes / Q000382:microbiology*; D010641:Phenotype; D013211:Staphylococcus aureus / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification / Q000502:physiology*; D017211:Treatment Failure
chemical_list: D004269:DNA, Bacterial