Regulation of nitric oxide induced by mycobacterial lipoarabinomannan in murine macrophages: effects of interferon-beta and taurine-chloramine.
We examined the effects of interferon beta (IFN-beta) on the production of liporabinomannan (LAM)-induced nitric oxide (NO) in peritoneal macrophages from low-responder and high-responder (C3H/HeJ and C3H/OuJ) mice. NO was produced in a dose response when induced by lipo-polysaccharide (LPS) or LAM plus interferon gamma (IFN-gamma) or IFN-beta in both high- and low-responder mice. In contrast to IFN-gamma, both high- and low-responder mice failed to induce nitrite production when IFN-beta was added, except at a high concentration of IFN-beta. Tau-Cl (0.5 mM) inhibited NO production about 50% in the high-responder strain when cells were activated with LPS or LAM in combination with either IFN-beta or IFN-gamma, and almost abolished NO production at 1.0 mM. In the low-responder strain, Tau-Cl (0.5 mM) significantly inhibited NO production when cells were activated with IFN-gamma or IFN-beta in addition to LPS or LAM, but did not completely inhibit NO production at 1.0 mM. Tau-Cl appears to play a potent role in regulating inflammatory reaction-induced bacterial or mycobacterial organisms. These data indicate a pivotal role for IFN-gamma and IFN-beta for the production of LPS and LAM initiated NO in peritoneal macrophages from low-responder (C3H/HeJ) mice.
Park
E
E
Department of Immunology, New York State Institute for Basic Research in Developmental Disabilities, 1050 Forest Hill Road, Staten Island, New York 10314, USA.
Levis
W R
WR
Quinn
M R
MR
Park
S Y
SY
Schuller-Levis
G B
GB
D000818:Animals; D004305:Dose-Response Relationship, Drug; D005260:Female; D018836:Inflammation Mediators / Q000494:pharmacology*; D007370:Interferon Type I / Q000494:pharmacology*; D007371:Interferon-gamma / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology*; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008809:Mice, Inbred C3H; D009161:Mycobacterium; D009569:Nitric Oxide / Q000037:antagonists & inhibitors / Q000378:metabolism*; D011994:Recombinant Proteins; D013654:Taurine / Q000031:analogs & derivatives* / Q000494:pharmacology*
D018836:Inflammation Mediators; D007370:Interferon Type I; D008070:Lipopolysaccharides; D011994:Recombinant Proteins; C050016:lipoarabinomannan; D013654:Taurine; D009569:Nitric Oxide; C043410:N-chlorotaurine; D007371:Interferon-gamma