Late preconditioning in rat retina: involvement of adenosine and ATP-sensitive K(+) channel.
To determine whether stimulation of adenosine receptors and opening of ATP-sensitive K(+) channels were involved in the protective effect of late preconditioning in the rat retina, rats were subjected to 60 min of retinal ischemia, and ischemic preconditioning was achieved by applying 5 min of ischemia 24 h before 60 min of ischemia. In non-preconditioned rats, cell loss in the ganglion cell layer and thinning of the inner plexiform and inner nuclear layer were observed 7 days after 60 min of ischemia. Ischemic preconditioning completely prevented the retinal tissue damage and 8-phenyltheophylline or 5-hydroxydecanoate reduced the protective effect of ischemic preconditioning. Therefore, stimulation of adenosine receptors and opening of ATP-sensitive K(+) channels might be involved in the mechanism of histological protection by late preconditioning in the retina.
Sakamoto
K
K
Department of Molecular Pharmacology, Kitasato University School of Pharmaceutical Sciences, 9-1 Shirokane 5-chome, Tokyo 108-8641, Minato-ku, Japan. sakamotok@pharm.kitasato-u.ac.jp
Kuwagata
M
M
Nakahara
T
T
Ishii
K
K
D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000818:Animals; D048429:Cell Size / Q000187:drug effects; D003652:Decanoic Acids / Q000494:pharmacology; D006880:Hydroxy Acids / Q000494:pharmacology; D015640:Ion Channel Gating / Q000187:drug effects*; D007511:Ischemia / Q000378:metabolism / Q000473:pathology; D019194:Ischemic Preconditioning; D008297:Male; D026902:Potassium Channel Blockers; D015221:Potassium Channels / Q000378:metabolism*; D058915:Purinergic P1 Receptor Antagonists; D051381:Rats; D017207:Rats, Sprague-Dawley; D018047:Receptors, Purinergic P1 / Q000378:metabolism*; D012160:Retina / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D013806:Theophylline / Q000031:analogs & derivatives* / Q000494:pharmacology
D003652:Decanoic Acids; D006880:Hydroxy Acids; D026902:Potassium Channel Blockers; D015221:Potassium Channels; D058915:Purinergic P1 Receptor Antagonists; D018047:Receptors, Purinergic P1; C052853:5-hydroxydecanoic acid; D000255:Adenosine Triphosphate; D013806:Theophylline; C028322:8-phenyltheophylline