title: Screening of Bcr-Abl transcripts in Philadelphia negative essential thrombocythemia.
abstract: Essential thrombocythemia (ET) is a chronic myeloproliferative disorder characterised by the absence of the Philadelphia (Ph+) chromosome. Recent studies have reported controversial results relating to BCR-ABL rearrangements in ET patients. We studied 44 Ph-negative ET patients with the RT-PCR technique at diagnosis or during the follow-up. None of them showed any of the BCR-ABL transcript actually described by others in ET; neither the "classical" P210 nor the P190 or P230 variants. Our results confirm the absence of BCR-ABL abnormalities in Ph-negative ET patients.
authors.affiliation: Chair and Division of Hematology, Department of Medical and Morphological Research, University of Udine, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Tiribelli
authors.forename: A
authors.initials: A
authors.lastname: Michelutti
authors.forename: G
authors.initials: G
authors.lastname: Damante
authors.forename: L
authors.initials: L
authors.lastname: Pellizzari
authors.forename: G
authors.initials: G
authors.lastname: Martinelli
authors.forename: M
authors.initials: M
authors.lastname: Amabile
authors.forename: D
authors.initials: D
authors.lastname: Russo
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D016044:Fusion Proteins, bcr-abl / Q000235:genetics*; D005820:Genetic Testing; D014644:Genetic Variation / Q000235:genetics; D006801:Humans; D008297:Male; D008875:Middle Aged; D010677:Philadelphia Chromosome; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013920:Thrombocythemia, Essential / Q000175:diagnosis / Q000235:genetics*
chemical_list: D012333:RNA, Messenger; D016044:Fusion Proteins, bcr-abl