Is the oxidation of high-density lipoprotein lipids different than the oxidation of low-density lipoprotein lipids?
This article gives detailed insight into the kinetics of high-density lipoprotein (HDL) oxidation catalyzed by azobis(2-amidinopropane).dihydrochloride (ABAP) or by copper. ABAP initialized oxidation of human HDL 3-4 times faster than non-human primate HDL with a similar composition. The oxidizability of non-human primate HDL was 1000 times lower than the oxidizability calculated from rate constants derived from liposome oxidation, suggesting that there is a slow step in HDL oxidation not present in liposomes. Saturable binding of copper to HDL was a significant feature of copper-catalyzed oxidation. Binding constants (K(m)) for non-human primate HDL were 2-3-fold lower than those for human HDL. Copper-catalyzed oxidation of non-human primate HDL was slower than that of human HDL, but human HDL(2) and HDL(3) oxidized at about the same rate. Overall, the kinetics describing the oxidation of HDL were mechanistically similar to those reported for LDL, suggesting that HDL lipids were as easily oxidized as LDL lipids and that HDL will be easily oxidized in vivo when exposed to agents that oxidize LDL.
Thomas
M J
MJ
Department of Biochemistry, Wake Forest University School of Medicine, Medical Center Blvd., Winston-Salem, NC 27157, USA. mthomas@wfubmc.edu
Chen
Q
Q
Zabalawi
M
M
Anderson
R
R
Wilson
M
M
Weinberg
R
R
Sorci-Thomas
M G
MG
Rudel
L L
LL
D000578:Amidines / Q000737:chemistry; D000818:Animals; D001665:Binding Sites; D002522:Chlorocebus aethiops; D002788:Cholesterol Esters / Q000737:chemistry / Q000378:metabolism; D003300:Copper / Q000737:chemistry; D005609:Free Radicals / Q000737:chemistry; D006801:Humans; D015227:Lipid Peroxidation; D008075:Lipoproteins, HDL / Q000737:chemistry / Q000378:metabolism*; D008077:Lipoproteins, LDL / Q000737:chemistry / Q000378:metabolism*; D016877:Oxidants / Q000737:chemistry; D012996:Solutions
D000578:Amidines; D002788:Cholesterol Esters; D005609:Free Radicals; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D016877:Oxidants; D012996:Solutions; C046728:2,2'-azobis(2-amidinopropane); D003300:Copper