A synthetic dDAF (CD55) gene based on optimal codon usage for transgenic animals.
The human DAF (CD55) gene was chosen as a representative molecule in a xenotransplantation study. The gene was synthesized in order to adapt its codons to those which are more frequent in mammals, especially pigs, and the expression levels were then examined in Chinese hamster ovarian (CHO) cells, swine endothelial cell (SEC) and transgenic mice. A significant increase in protein production with no detectable mRNA elevation was observed in the transfectants of synthetic DAF (sDAF), compared with the wild-type DAF (wtDAF) and delta-SCR1 wild-type DAF (Delta1wtDAF). Consistent with the in vitro data, the expression of DAF in mice that carry sDAF was higher than Delta1wtDAF in many organs, especially the pancreas. The sDAF showed a high level of expression in SEC and transgenic mice, suggesting that it will be useful in the development of transgenic pigs with high levels of expression.
Miyagawa
S
S
Division of Organ Transplantation, Osaka University Graduate School of Medicine, Yamadaoka, Suita, Osaka 565-0871, Japan. miyagawa@orgtrp.med.osaka-u.ac.jp
Yamada
M
M
Matsunami
K
K
Koresawa
Y
Y
Ikawa
M
M
Okabe
M
M
Shirakura
R
R
D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics / Q000378:metabolism; D018958:CD55 Antigens / Q000096:biosynthesis* / Q000235:genetics*; D016466:CHO Cells / Q000378:metabolism*; D003062:Codon / Q000235:genetics; D006224:Cricetinae; D004727:Endothelium / Q000166:cytology / Q000378:metabolism; D015870:Gene Expression / Q000235:genetics; D005813:Genes, Synthetic / Q000235:genetics*; D008322:Mammals / Q000235:genetics; D051379:Mice; D008822:Mice, Transgenic / Q000235:genetics* / Q000378:metabolism; D010179:Pancreas / Q000378:metabolism; D013552:Swine / Q000235:genetics*; D014162:Transfection / Q000379:methods; D014183:Transplantation, Heterologous / Q000379:methods
D018958:CD55 Antigens; D003062:Codon