Polymerase chain reaction for detection of Legionellae DNA in urine samples from patients with community-acquired pneumonia.
Polymerase chain reaction (PCR) was used for detecting Legionella DNA in water, sputum, tracheal aspirate and bronchoalveolar lavage fluid. There is paucity of data on the use of PCR for detection of Legionella in serum and urine samples. In 82 patients admitted with community-acquired pneumonia, urinary PCR was used in addition to urinary antigen assay for Legionella pneumophila serogroup 1 and serological tests (indirect immunofluorescence and ELISA) in paired sera. PCR was positive in urine samples from 21 patients (26%): in six of seven patients with acute legionellosis by CDC criteria, and 15 patients with negative urine antigen showing no fourfold rise in antibody titers in immunofluorescence test.
Socan
M
M
Department of Infectious Diseases, University Medical Centre, Ljubljana, Slovenia. maja.socan@ivz-rs.si
Kese
D
D
Marinic-Fiser
N
N
D000293:Adolescent; D000328:Adult; D000368:Aged; D000907:Antibodies, Bacterial / Q000097:blood; D017714:Community-Acquired Infections / Q000382:microbiology; D004269:DNA, Bacterial / Q000097:blood / Q000652:urine*; D004797:Enzyme-Linked Immunosorbent Assay; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D016952:Legionella pneumophila / Q000235:genetics / Q000302:isolation & purification*; D007877:Legionnaires' Disease / Q000175:diagnosis* / Q000382:microbiology; D008875:Middle Aged; D018410:Pneumonia, Bacterial / Q000175:diagnosis* / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D014556:Urine / Q000382:microbiology*
D000907:Antibodies, Bacterial; D004269:DNA, Bacterial