title: Functional complementation of pyran ring formation in actinorhodin biosynthesis in Streptomyces coelicolor A3(2) by ketoreductase genes for granaticin biosynthesis.
abstract: A mutation in actVI-ORF1, which controls C-3 reduction in actinorhodin biosynthesis by Streptomyces coelicolor, was complemented by gra-ORF5 and -ORF6 from the granaticin biosynthetic gene cluster of Streptomyces violaceoruber Tü22. It is hypothesized that, while gra-ORF5 alone is a ketoreductase for C-9, gra-ORF6 gives the enzyme regiospecificity also for C-3.
authors.affiliation: Graduate School of Pharmaceutical Sciences, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan. ichinose@mol.f.u.tokyo.ac.jp
authors.forename: K
authors.initials: K
authors.lastname: Ichinose
authors.forename: T
authors.initials: T
authors.lastname: Taguchi
authors.forename: D J
authors.initials: DJ
authors.lastname: Bedford
authors.forename: Y
authors.initials: Y
authors.lastname: Ebizuka
authors.forename: D A
authors.initials: DA
authors.lastname: Hopwood
mesh_terms: D000429:Alcohol Oxidoreductases / Q000235:genetics* / Q000378:metabolism; D000880:Anthraquinones / Q000737:chemistry / Q000378:metabolism*; D001426:Bacterial Proteins; D003227:Conjugation, Genetic; D005816:Genetic Complementation Test; D009154:Mutation; D009285:Naphthoquinones / Q000378:metabolism*; D011714:Pyrans / Q000378:metabolism*; D013302:Streptomyces / Q000235:genetics / Q000378:metabolism*; D000095622:Benzoisochromanequinones
chemical_list: D000429:Alcohol Oxidoreductases; D000880:Anthraquinones; D001426:Bacterial Proteins; D009285:Naphthoquinones; D011714:Pyrans; C009811:granaticin; D000095622:Benzoisochromanequinones; C087192:polyketide synthase ketoreductase; C013390:actinorhodin