title: New method for determination of ten pesticides in human blood.
abstract: An analytical method was developed for precise identification and quantitation of 10 pesticides in human blood. The pesticides studied, which have appeared frequently in actual cases, were endosulfan, lindane, parathion, ethyl-azinphos, diazinon, malathion, alachlor, tetradifon, fenthion and dicofol (o-p' and p-p' isomers). The current method replaces an earlier method which involved liquid-liquid extraction with a mixture of n-hexane-benzene (1 + 1). The extraction is performed by solid-phase extraction, with C18 cartridges and 2 internal standards, perthane and triphenylphosphate. Eluates were analyzed by gas chromatography (GC) with nitrogen-phosphorus and electrochemical detectors. Results were confirmed by GC-mass spectrometry in the electron impact mode. Blood blank samples spiked with 2 standard mixtures and an internal standard were used for quantitation. Mean recoveries ranged from 71.83 to 97.10%. Detection and quantitation limits are reported for each pesticide. Examples are provided to show the application of the present method to actual samples.
authors.affiliation: Instituto Nacional de Toxicología, Departamento de Sevilla, Spain.
authors.forename: R
authors.initials: R
authors.lastname: García-Repetto
authors.forename: M P
authors.initials: MP
authors.lastname: Giménez
authors.forename: M
authors.initials: M
authors.lastname: Repetto
mesh_terms: D002138:Calibration; D002849:Chromatography, Gas; D004563:Electrochemistry; D005554:Forensic Medicine; D006801:Humans; D013058:Mass Spectrometry; D010575:Pesticides / Q000097:blood* / Q000506:poisoning; D011041:Poisoning / Q000175:diagnosis; D012015:Reference Standards; D015203:Reproducibility of Results
chemical_list: D010575:Pesticides