One-stage full-mouth disinfection. Long-term microbiological results analyzed by checkerboard DNA-DNA hybridization.
BACKGROUND
Recent studies reported significant, additional clinical and microbiological improvements when severe adult periodontitis was treated via the one-stage full-mouth (OSFM) disinfection approach, instead of a standard treatment scheme with staged instrumentation per quadrant. The OSFM disinfection involves dealing with the remaining oropharyngeal niches such as tonsils, saliva, tongue, and mucosa. The OSFM disinfection procedure involves scaling and root planing of all pockets within 24 hours in combination with chlorhexidine application to all oropharyngeal niches (chairside and at home for 2 months). This study aimed to compare the microbiological shifts with the OSFM approach versus standard therapy.


METHODS
Nineteen patients with advanced chronic periodontitis (AP) and 12 patients with early-onset periodontitis (EOP) were randomly assigned to the test and control groups. The control group (9 AP patients, 6 EOP patients) was scaled and root planed, per quadrant, with 2-week intervals. The test group (10 AP patients and 6 EOP patients) underwent OSFM disinfection treatment. At baseline and after 2, 4, and 8 months, pooled subgingival plaque samples were taken from single- and multi-rooted teeth. The presence and levels of 30 subgingival taxa were determined using whole genomic DNA probes and checkerboard DNA-DNA hybridization.


RESULTS
Both treatments resulted in important reductions of the pathogenic species up to 8 months after therapy, both for their detection level and frequency. The OSFM disinfection resulted in an additional improvement, especially in the AP group. P. gingivalis and B. forsythus were reduced below detection level. The number of beneficial species remained nearly unchanged.


CONCLUSIONS
The OSFM disinfection results in supplementary reductions of periodontal pathogens even after 8 months in the treatment of patients with advanced or early-onset periodontitis.
De Soete
M
M
Catholic University of Leuven, Faculty of Medicine, School of Dentistry, Oral Pathology and Maxillo-Facial Surgery, Department of Periodontology, Belgium.
Mongardini
C
C
Peuwels
M
M
Haffajee
A
A
Socransky
S
S
van Steenberghe
D
D
Quirynen
M
M
D000328:Adult; D000368:Aged; D010520:Aggressive Periodontitis / Q000382:microbiology / Q000628:therapy; D000891:Anti-Infective Agents, Local / Q000008:administration & dosage / Q000627:therapeutic use; D001419:Bacteria / Q000187:drug effects / Q000235:genetics / Q000254:growth & development*; D001439:Bacteroides / Q000187:drug effects / Q000254:growth & development; D002710:Chlorhexidine / Q000008:administration & dosage / Q000627:therapeutic use; D002908:Chronic Disease; D012534:Dental Scaling; D004203:Disinfection / Q000379:methods*; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008297:Male; D008875:Middle Aged; D009055:Mouth / Q000382:microbiology*; D009061:Mouth Mucosa / Q000382:microbiology; D009693:Nucleic Acid Hybridization; D009960:Oropharynx / Q000382:microbiology; D014066:Palatine Tonsil / Q000382:microbiology; D010514:Periodontal Pocket / Q000382:microbiology / Q000628:therapy; D010518:Periodontitis / Q000382:microbiology / Q000628:therapy*; D016966:Porphyromonas gingivalis / Q000187:drug effects / Q000254:growth & development; D016745:Root Planing; D012463:Saliva / Q000382:microbiology; D014059:Tongue / Q000382:microbiology
D000891:Anti-Infective Agents, Local; D002710:Chlorhexidine