Identification of Candida dubliniensis based on the specific amplification of mitochondrial cytochrome b gene.
Candida dubliniensis, a recently described Candida species, is frequently isolated from oral candidiasis in human immunodeficiency virus infected individuals. To detect the organism rapidly, we have developed specific oligonucleotide primers based on the sequences of mitochondrial cytochrome b gene. These primers selectively amplified DNA only from C. dubliniensis; the DNAs of all pathogenic Candida species tested, as well as those of medically relevant yeasts such as Cryptococcus neoformans, and Trichosporon cutaneum, were not amplified. This is the first report describing the effectiveness of cytochrome b gene in PCR based detection of an organism, and we hope the system will be useful as a microbiological tool for rapid detection of C. dubliniensis.
Biswas
S K
SK
Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, 1-8-1 Inohana Chuo-ku, Chiba 260-8673, Japan.
Yokoyama
K
K
Wang
L
L
Nishimura
K
K
Miyaji
M
M
D001483:Base Sequence; D002175:Candida / Q000302:isolation & purification*; D002180:Candidiasis, Oral / Q000382:microbiology; D003573:Cytochrome b Group / Q000235:genetics*; D017931:DNA Primers; D006801:Humans; D008928:Mitochondria / Q000201:enzymology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
D003573:Cytochrome b Group; D017931:DNA Primers