title: Effect of six flavonoids on proliferation of hepatic stellate cells in vitro.
abstract: OBJECTIVE
  To study the effects of six flavonoids (fisetin, quercetin, apigenin, phloretin, hesperetin, and chalcone) on proliferation of hepatic stellate cell (HSC-T6 cells).
  
  
  METHODS
  Cell proliferation was measured by crystal violet staining assay.
  
  
  RESULTS
  Fisetin, quercetin, apigenin, phloretin, hesperetin, chalcone (6.25-50 mumol.L-1) inhibited the proliferation of HSC-T6 cells stimulated by serum, macrophage conditioned medium (MCM) and platelet-derived growth factor (PDGF) in a concentration-dependent manner. In the MCM-stimulated proliferation experiment, their IC50 were 21.48, 18.52, 19.75, 22.32, 30.32, and 30.85 mumol.L-1, respectively. In the PDGF-stimulated proliferation experiment, their IC50 were 9.47, 9.48, 9.25, 12.25, 25.22, and 30.40 mumol.L-1, respectively.
  
  
  CONCLUSIONS
  The six flavonoids inhibited the proliferation of hepatic stellate cells.
authors.affiliation: Department of Biochemical Pharmacy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China.
authors.forename: M
authors.initials: M
authors.lastname: Zhang
authors.forename: J P
authors.initials: JP
authors.lastname: Zhang
authors.forename: H T
authors.initials: HT
authors.lastname: Ji
authors.forename: J S
authors.initials: JS
authors.lastname: Wang
authors.forename: D H
authors.initials: DH
authors.lastname: Qian
mesh_terms: D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D017077:Culture Media, Conditioned; D005260:Female; D005419:Flavonoids / Q000494:pharmacology*; D044948:Flavonols; D006569:Hesperidin; D007728:Kupffer Cells / Q000166:cytology / Q000187:drug effects*; D051379:Mice; D008813:Mice, Inbred ICR; D010982:Platelet-Derived Growth Factor / Q000037:antagonists & inhibitors; D011794:Quercetin / Q000494:pharmacology
chemical_list: D017077:Culture Media, Conditioned; D005419:Flavonoids; D044948:Flavonols; D010982:Platelet-Derived Growth Factor; D011794:Quercetin; D006569:Hesperidin; C017875:fisetin; C013015:hesperetin