Comparison of the growth inhibitory effects of canine IFN-alpha, -beta and -gamma on canine cells infected with Neospora caninum tachyzoites.
The growth inhibitory effects of recombinant canine interferon alpha (IFN-alpha), beta (IFN-beta) and gamma (IFN-gamma) were examined on Madin-Darby canine kidney cells infected with Neospora caninum tachyzoites. The parasite growth was inhibited by all IFNs in a dose-dependent manner. IFN-gamma inhibited the parasite growth with greater efficacy than IFN-alpha or IFN-beta. Moreover, the effect of IFNs on N. caninum growth associated with the suppression of the host cell viability. The present study indicates IFN-alpha and -beta, besides IFN-gamma, play a crucial role for N. caninum growth in host cells.
Nishikawa
Y
Y
National Research Center for Protozoan Diseases, Obihiro University, Hokkaido, Japan.
Iwata
A
A
Nagasawa
H
H
Fujisaki
K
K
Otsuka
H
H
Mikami
T
T
D000818:Animals; D016367:Baculoviridae / Q000737:chemistry / Q000235:genetics; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003001:Cloning, Molecular; D003048:Coccidiosis / Q000188:drug therapy / Q000662:veterinary*; D004247:DNA / Q000737:chemistry; D004283:Dog Diseases / Q000469:parasitology*; D004285:Dogs; D006801:Humans; D007372:Interferons / Q000494:pharmacology*; D007668:Kidney / Q000166:cytology / Q000469:parasitology; D018688:Neospora / Q000187:drug effects* / Q000254:growth & development; D011994:Recombinant Proteins / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary
D011994:Recombinant Proteins; D004247:DNA; D007372:Interferons