title: Cloning and characterization of tissue inhibitor of metalloproteinase-3 (TIMP-3) from shark, Scyliorhinus torazame.
abstract: We cloned the full-length cDNA encoding TIMP-3 from the cartilage of cloudy dogfish, Scyliorhinus torazame. The entire open reading frame was composed of 645 nucleotides and 214 residues including 12 conserved cysteines and asparagine-184, a putative site for N-linked sugars. It showed about 72% identity to those of other species based on the deduced amino acid sequence. The mRNA of shark TIMP-3 were expressed abundantly in brain and cartilage tissues. To investigate the roles of shark TIMP-3, an expression vector was constructed and transfected into HT1080 human fibrosarcoma cells. Overexpression of shark TIMP-3 reduced the activity of MMP-2 in gelatin zymography. Through human Alu PCR based CAM assay, we also confirmed that shark TIMP-3 transfected HT1080 cells had less intravasation effects.
authors.affiliation: Department of Molecular Biology, Pusan National University, Pusan 609-735, South Korea.
authors.forename: J T
authors.initials: JT
authors.lastname: Kim
authors.forename: M S
authors.initials: MS
authors.lastname: Kim
authors.forename: M K
authors.initials: MK
authors.lastname: Bae
authors.forename: H S
authors.initials: HS
authors.lastname: Song
authors.forename: M Y
authors.initials: MY
authors.lastname: Ahn
authors.forename: Y J
authors.initials: YJ
authors.lastname: Kim
authors.forename: S J
authors.initials: SJ
authors.lastname: Lee
authors.forename: K W
authors.initials: KW
authors.lastname: Kim
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002356:Cartilage / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000096:biosynthesis / Q000737:chemistry; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D012754:Sharks / Q000235:genetics* / Q000378:metabolism; D019717:Tissue Inhibitor of Metalloproteinase-3 / Q000737:chemistry / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D019717:Tissue Inhibitor of Metalloproteinase-3