title: Direct somatic embryogenesis and plantlet regeneration from leaf explants of niger, Guizotia abyssinica (L.f.) Cass.
abstract: Somatic embryogenesis was induced in niger (Guizotia abyssinica (L.f.) Cass.) using a simple one step method. Leaf explants were cultured on MS medium with 2,4-D and BAP/Kn individually and in combination. Somatic embryogenesis occurred directly without an intervening callus proliferation phase from subepidermal regions of leaf explants on MS medium supplemented with 2,4-D (2.0 and 5.0 microM) plus Kn/BAP (1.0 and 2.0 microM). Regenerated somatic embryos were successfully grown into whole plants.
authors.affiliation: Plant Tissue Culture Laboratory, Department of Botany, Karnatak University, Dharwad 580003, India.
authors.forename: H G
authors.initials: HG
authors.lastname: Kumar
authors.forename: H N
authors.initials: HN
authors.lastname: Murthy
authors.forename: V G
authors.initials: VG
authors.lastname: Jadimath
authors.forename: S S
authors.initials: SS
authors.lastname: Sheelavantmath
authors.forename: A N
authors.initials: AN
authors.lastname: Pyati
authors.forename: B V
authors.initials: BV
authors.lastname: Ravishankar
mesh_terms: D019659:Asteraceae / Q000166:cytology / Q000196:embryology* / Q000254:growth & development; D002455:Cell Division; D018515:Plant Leaves / Q000254:growth & development*; D012038:Regeneration; D012639:Seeds / Q000254:growth & development