Application of the ethoxyresorufin-O-deethylase (EROD) assay to mixtures of halogenated aromatic compounds.
The ethoxyresorufin-O-deethylase (EROD) assay monitors the induction of the xenobiotic-metabolizing enzyme cytochrome P-450 (CYP) 1A1 and is a widely used biomarker for exposure of wildlife to substances that bind the aryl hydrocarbon (Ah) receptor. In this work the induction of EROD activity by single compounds and binary mixtures in primary rat hepatocytes was compared with the predictions of a kinetic model involving mixtures of inducers. The inducing agents were also examined for their ability to activate the Ah receptor to its DNA-binding form and for their ability to act as competitive inhibitors for CYP 1A1. Xenobiotics that failed to activate the Ah receptor did not induce EROD activity. Competitive inhibition for CYP 1A1 between the xenobiotic and 7-ethoxyresorufin caused EROD activity to fall at high xenobiotic concentrations. Competition for a limited number of Ah receptor sites depressed the EROD activity of a strong inducer such as 2,3,7,8-tetrachlorodibenzo-p-dioxin at high concentrations of a weak inducer. Application of the kinetic model to the example of a mixture of low concentrations of dibenzo-p-dioxins and much higher concentrations of polychlorinated biphenyls indicated that EROD assays often seriously underestimate the true potency of an environmental sample. Hence the EROD assay cannot be used for determining dioxin equivalent concentrations using the toxic equivalence factor approach.
Petrulis
J R
JR
Department of Chemistry and Biochemistry, University of Guelph, Ontario, Canada.
Chen
G
G
Benn
S
S
LaMarre
J
J
Bunce
N J
NJ
D000818:Animals; D001681:Biological Assay / Q000592:standards*; D019363:Cytochrome P-450 CYP1A1; D004147:Dioxins / Q000633:toxicity*; D004784:Environmental Monitoring / Q000592:standards*; D022781:Hepatocytes / Q000187:drug effects*; D006846:Hydrocarbons, Halogenated / Q000633:toxicity*; D015233:Models, Statistical; D051381:Rats; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D004147:Dioxins; D006846:Hydrocarbons, Halogenated; D014874:Water Pollutants, Chemical; D019363:Cytochrome P-450 CYP1A1