A new multiplex PCR strategy for the simultaneous determination of four genetic polymorphisms affecting HIV-1 disease progression.
The CCR5 Delta32, CCR2 64I, SDF1 3'A, and CCR5 promoter 59029 polymorphisms have been suggested to influence HIV-1 disease progression. Furthermore, the CCR5 Delta32 and the CCR2 64I polymorphisms have been associated with various other diseases. The purpose of the present study was to develop a multiplex assay for the simultaneous determination of these four polymorphisms. Results obtained with the multiplex assay were compared to results obtained by conventional RFLP-PCR and no differences were observed. The multiplex assay offers a quick tool for the determination of the CCR5 Delta32, CCR2 64I, SDF1 3'A, and CCR5 promoter 59029 A/G polymorphisms.
Kristiansen
T B
TB
Clinical Research Unit 136, Copenhagen University Hospitals Hvidovre, Kettegaard Alle 30, 2650 Hvidovre, Copenhagen, Denmark.
Knudsen
T B
TB
Ohlendorff
S
S
Eugen-Olsen
J
J
D054377:Chemokine CXCL12; D019743:Chemokines, CXC / Q000235:genetics*; D018450:Disease Progression; D015658:HIV Infections / Q000235:genetics* / Q000503:physiopathology; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic; D054390:Receptors, CCR2; D019713:Receptors, CCR5 / Q000235:genetics*; D019707:Receptors, Chemokine / Q000235:genetics*
C515334:CCR2 protein, human; C515255:CXCL12 protein, human; D054377:Chemokine CXCL12; D019743:Chemokines, CXC; D054390:Receptors, CCR2; D019713:Receptors, CCR5; D019707:Receptors, Chemokine