title: Prolactin signalling to milk protein secretion but not to gene expression depends on the integrity of the Golgi region.
abstract: Prolactin added to the incubation medium of lactating mammary epithelial cells is transported from the basal to the apical region of cells through the Golgi region and concomitantly stimulates arachidonic acid release and protein milk secretion. We report that when PRL is added after disorganisation of the Golgi apparatus by brefeldin A treatment, prolactin signalling to expression of genes for milk proteins and prolactin endocytosis are not affected. However, prolactin transport to the apical region of cells (transcytosis), as well as prolactin-induced arachidonic acid release and subsequent stimulation of the secretion of caseins, which are located in a post-Golgi compartment, are inhibited. This inhibition was not a consequence of damage to the secretory machinery, as under the same conditions, protein secretion could be stimulated by the addition of arachidonic acid to the incubation medium. Thus, it is possible to discriminate between prolactin-induced actions that are dependent (signalling to milk protein secretion) or independent (signalling to milk gene expression) on the integrity of the Golgi apparatus. These results suggest that these two biological actions may be transduced via distinct intracellular pathways, and support the hypothesis that prolactin signals may be emitted at various cellular sites.
authors.affiliation: Faculté des Sciences, UCD, El Jadida, Maroc.
authors.forename: M
authors.initials: M
authors.lastname: Lkhider
authors.forename: B
authors.initials: B
authors.lastname: Pétridou
authors.forename: A
authors.initials: A
authors.lastname: Aubourg
authors.forename: M
authors.initials: M
authors.lastname: Ollivier-Bousquet
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D016718:Arachidonic Acid / Q000493:pharmacokinetics; D001940:Breast / Q000166:cytology; D020126:Brefeldin A / Q000494:pharmacology; D002250:Carbon Radioisotopes; D004268:DNA-Binding Proteins / Q000378:metabolism; D004705:Endocytosis / Q000502:physiology; D018870:Endoplasmic Reticulum, Rough / Q000502:physiology; D005260:Female; D015870:Gene Expression / Q000502:physiology; D006056:Golgi Apparatus / Q000187:drug effects / Q000502:physiology*; D066298:In Vitro Techniques; D007774:Lactation / Q000502:physiology*; D018942:Macrolides; D008894:Milk Proteins / Q000235:genetics* / Q000378:metabolism*; D010766:Phosphorylation; D011388:Prolactin / Q000493:pharmacokinetics*; D011817:Rabbits; D011981:Receptors, Prolactin / Q000378:metabolism; D050799:STAT5 Transcription Factor; D022142:Secretory Vesicles / Q000502:physiology; D015398:Signal Transduction / Q000502:physiology*; D015534:Trans-Activators / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D002250:Carbon Radioisotopes; D004268:DNA-Binding Proteins; D018942:Macrolides; D008894:Milk Proteins; D011981:Receptors, Prolactin; D050799:STAT5 Transcription Factor; D015534:Trans-Activators; D020126:Brefeldin A; D016718:Arachidonic Acid; D011388:Prolactin