Identification of Actinobacillus actinomycetemcomitans serotypes by multiplex PCR.
Oligonucleotide primers specific for gene clusters involved in the biosynthesis of serotype-specific polysaccharide antigens were designed to identify Actinobacillus actinomycetemcomitans serotypes a to e using the multiplex PCR. This method may be useful for serotype-specific genotyping rapidly and directly from clinical samples containing various organisms.
Suzuki
N
N
Department of Preventive Dentistry, Kyushu University Faculty of Dental Science, Fukuoka 812-8582, Japan.
Nakano
Y
Y
Yoshida
Y
Y
Ikeda
D
D
Koga
T
T
D000189:Actinobacillus Infections / Q000382:microbiology*; D016976:Aggregatibacter actinomycetemcomitans / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D015373:Bacterial Typing Techniques; D006801:Humans; D010518:Periodontitis / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012703:Serotyping