Soluble p185/her2 and S100 in yolk sac blood from human melanoma metastases xenotransplanted to chick embryo chorioallantoic membrane.
BACKGROUND
The chorionallantoic membrane (CAM) of the chick embryo has been used as an experimental model for studying tumor invasion and metastasis of human malignant melanoma. In search for a model to show graft-host-interactions in vivo, tumor markers in peripheral blood of the host were investigated.


METHODS
Before collecting melanoma metastasis xenografts, blood samples were taken from CAM and a control group. S100 and sp185/her2 in peripheral blood were evaluated in a blinded manner.


RESULTS
23/28 samples deriving from successfully performed human melanoma metastasis CAM xenografts were positive for S100 versus 2/22 samples for sp185/her2.


CONCLUSIONS
Regarding melanoma, in this model sp185/her2 gave no additional information. S100 levels corresponded to clinical and immunohistological findings concerning adherence of tumors and extravasation of human melanoma cells. Based on these data S100 levels in the peripheral blood could help to determine the effect of exogenous stimuli such as radiation and therapeutic agents on metastatisation of the xenografts.
Kaskel
P
P
Department of Dermatology, University of Ulm, Ulm/Donau, Germany. kaskel@medizin.uni-ulm.de
Kunzi-Rapp
K
K
Leiter
U
U
Peter
R U
RU
Krähn
G
G
D000818:Animals; D002642:Chick Embryo; D006801:Humans; D008545:Melanoma / Q000097:blood* / Q000473:pathology; D008954:Models, Biological; D009362:Neoplasm Metastasis; D009368:Neoplasm Transplantation; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000097:blood*; D009418:S100 Proteins / Q000097:blood*; D014183:Transplantation, Heterologous; D015017:Yolk Sac / Q000098:blood supply / Q000378:metabolism
D018719:Erb-b2 Receptor Tyrosine Kinases; D009418:S100 Proteins; C110294:S100A1 protein