Active and selective transcytosis of cell-free human immunodeficiency virus through a tight polarized monolayer of human endometrial cells.
We report that both primary and laboratory-adapted infectious human immunodeficiency virus type 1 (HIV-1) isolates in a cell-free form are capable of transcytosis through a tight and polarized monolayer of human endometrial cells. Trancytosis of cell-free HIV occurs in a strain-selective fashion and appears to be dependent on interactions between HIV envelope glycoproteins and lectins on the apical membrane of the epithelial cells. These findings provide new insights into the initial events occurring during heterosexual transmission of the virus.
Hocini
H
H
INSERM U430, Paris, France. hakim.hocini@brs.ap-hop-paris.fr
Becquart
P
P
Bouhlal
H
H
Chomont
N
N
Ancuta
P
P
Kazatchkine
M D
MD
Bélec
L
L
D002474:Cell-Free System; D003584:Cytological Techniques; D004717:Endometrium / Q000166:cytology / Q000821:virology*; D005260:Female; D005455:Fluorescent Antibody Technique; D019302:HIV Envelope Protein gp160 / Q000032:analysis; D015658:HIV Infections / Q000635:transmission* / Q000821:virology; D015497:HIV-1 / Q000302:isolation & purification / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D066298:In Vitro Techniques; D013696:Temperature; D014407:Tumor Cells, Cultured
D019302:HIV Envelope Protein gp160