Evaluation of oxidative stress during apoptosis and necrosis caused by carbon tetrachloride in rat liver.
After 12, 18, and 24 h of oral administration of carbon tetrachloride (as a 1:1 mixture with mineral oil: 4 ml/kg body weight) to rats, the activity of caspase-3-like protease in the liver increased significantly compared to that in the control group that was given mineral oil (4 ml/kg). In plasma, the activity of caspase-3 was barely detectable in the control rat, but increased significantly 24 h after drug administration along with a dramatic increase in glutamate oxaloacetate transaminase. These results indicate that carbon tetrachloride causes apoptosis in the liver by activating caspase-3, which is released to plasma by secondary necrosis. After 18 and 24 h of carbon tetrachloride administration, the liver concentration of hydrophilic vitamin C was decreased significantly, while that of hydrophobic vitamin E was not affected. The plasma concentration of vitamins C and E was not influenced significantly. These results suggest that carbon tetrachloride induces oxidative stress mainly in the aqueous phase of the liver cell.
Sun
F
F
Department of Food Science and Nutrition, Nara Women's University, 630-8506, Nara, Japan.
Hamagawa
E
E
Tsutsui
C
C
Ono
Y
Y
Ogiri
Y
Y
Kojo
S
S
D000818:Animals; D017209:Apoptosis; D001205:Ascorbic Acid / Q000032:analysis / Q000097:blood; D002251:Carbon Tetrachloride / Q000633:toxicity*; D053148:Caspase 3; D020169:Caspases / Q000032:analysis / Q000097:blood; D056487:Chemical and Drug Induced Liver Injury, Chronic / Q000209:etiology* / Q000378:metabolism / Q000473:pathology; D004789:Enzyme Activation / Q000187:drug effects; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D009336:Necrosis; D018384:Oxidative Stress; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D014810:Vitamin E / Q000032:analysis / Q000097:blood
D014810:Vitamin E; D002251:Carbon Tetrachloride; C505410:Casp3 protein, rat; D053148:Caspase 3; D020169:Caspases; D001205:Ascorbic Acid