title: Differential vulnerability of basal and apical hair cells is based on intrinsic susceptibility to free radicals.
abstract: The base of the cochlea is more vulnerable to trauma than the apex as seen in the pattern of hair cell damage by cisplatin or aminoglycosides. The differential vulnerability is maintained in organotypic cultures exposed directly to these drugs, suggesting there may be an intrinsic difference in sensitivity to damage along the cochlear spiral. We therefore investigated the survival capacity of isolated outer hair cells and strips dissected from different turns of the guinea pig organ of Corti in short-term culture. Cells were stained with fluorescent indicators of viable or dead cells, calcein-AM and ethidium homodimer. After 5 h at room temperature, up to 90% of outer hair cells from the apex survived, but less than 30% from the base. In contrast, basal inner hair cells remained viable, and supporting cells survived for at least 20 h. The difference in survival capacity between basal and apical outer hair cells coincided with a significantly lower level of the antioxidant glutathione in basal outer hair cells compared with apical outer hair cells. This suggested that basal outer hair cells may be more vulnerable to free-radical damage than apical outer hair cells. The survival of basal outer hair cells was significantly improved by addition of the radical scavengers n-acetyl cysteine, p-phenylenediamine, glutathione, mannitol or salicylate. The protection by antioxidants implies that the accelerated death of basal outer hair cells is due to free-radical damage. The results support an intrinsic susceptibility to free radicals that differs among cochlear cell populations. This differential provides a rational explanation for base-to-apex gradients observed in various forms of cochlear pathology.
authors.affiliation: Kresge Hearing Research Institute, University of Michigan, MI 48109-0506, USA.
authors.forename: S H
authors.initials: SH
authors.lastname: Sha
authors.forename: R
authors.initials: R
authors.lastname: Taylor
authors.forename: A
authors.initials: A
authors.lastname: Forge
authors.forename: J
authors.initials: J
authors.lastname: Schacht
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D016166:Free Radical Scavengers / Q000494:pharmacology; D005609:Free Radicals / Q000633:toxicity; D005978:Glutathione / Q000378:metabolism / Q000494:pharmacology; D006168:Guinea Pigs; D018072:Hair Cells, Auditory, Outer / Q000187:drug effects* / Q000293:injuries / Q000473:pathology; D066298:In Vitro Techniques; D008353:Mannitol / Q000494:pharmacology
chemical_list: D016166:Free Radical Scavengers; D005609:Free Radicals; D008353:Mannitol; D005978:Glutathione