title: A 49-residue peptide from adhesin F1 of Streptococcus pyogenes inhibits fibronectin matrix assembly.
abstract: F1 is an adhesin of Streptococcus pyogenes which binds the N-terminal 70-kDa region of fibronectin with high affinity. The fibronectin binding region of F1 is comprised of a 43-residue upstream domain and a repeat domain comprised of five tandem 37-residue sequences. We investigated the effects of these domains on the assembly of fibronectin matrix by human dermal fibroblasts, MG63 osteosarcoma cells, or fibroblasts derived from fibronectin-null stem cells. Subequimolar or equimolar concentrations of recombinant proteins containing both the upstream and repeat domains or just the repeat domain enhanced binding of fibronectin or its N-terminal 70-kDa fragment to cell layers; higher concentrations of these recombinant proteins inhibited binding. The enhanced binding did not result in greater matrix assembly and was caused by increased ligand binding to substratum. In contrast, recombinant or synthetic protein containing the 43 residues of the upstream domain and the first 6 residues from the repeat domain exhibited monophasic inhibition with an IC(50) of approximately 10 nm. Truncation of the 49-residue sequence at its N or C terminus caused loss of inhibitory activity. The 49-residue upstream sequence blocked incorporation of both endogenous cellular fibronectin and exogenous plasma fibronectin into extracellular matrix and inhibited binding of 70-kDa fragment to fibronectin-null cells in a fibronectin-free system. Inhibition of matrix assembly by the 49-mer had no effect on cell adhesion to substratum, cell growth, formation of focal contacts, or formation of stress fibers. These results indicate that the 49-residue upstream sequence of F1 binds in an inhibitory mode to N-terminal parts of exogenous and endogenous fibronectin which are critical for fibronectin fibrillogenesis.
authors.affiliation: Department of Medicine and the Molecular and Cellular Pharmacology Program, University of Wisconsin, Madison, Wisconsin 53706, USA.
authors.forename: B R
authors.initials: BR
authors.lastname: Tomasini-Johansson
authors.forename: N R
authors.initials: NR
authors.lastname: Kaufman
authors.forename: M G
authors.initials: MG
authors.lastname: Ensenberger
authors.forename: V
authors.initials: V
authors.lastname: Ozeri
authors.forename: E
authors.initials: E
authors.lastname: Hanski
authors.forename: D F
authors.initials: DF
authors.lastname: Mosher
mesh_terms: D018829:Adhesins, Bacterial / Q000737:chemistry*; D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002448:Cell Adhesion; D002455:Cell Division; D002478:Cells, Cultured; D005109:Extracellular Matrix / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000648:ultrastructure; D005353:Fibronectins / Q000378:metabolism*; D022001:Focal Adhesions / Q000648:ultrastructure; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D010455:Peptides / Q000494:pharmacology; D013297:Streptococcus pyogenes / Q000472:pathogenicity*; D022502:Stress Fibers / Q000648:ultrastructure; D014407:Tumor Cells, Cultured
chemical_list: D018829:Adhesins, Bacterial; D005353:Fibronectins; D010455:Peptides