Dietary antioxidative potential in arteries.
Many researches show that traditional Indonesian diet is good for physical health. The present study examines the antioxidative, anti-inflammatory and antithrombotic potentials of aqueous extract of tempe (fermented soya-beans) and Curcuma domestica in an experimental photochemical thrombogenesis model using rat femoral artery. A total of 15 male Wistar rats weighing 250 g were used, and divided into three groups: control (group-1, n = 5), animals, treated orally with 0.25 ml aqueous extracts of 50 g tempe (fermented soybean cake) once daily for 7 consecutive days (group-2, n = 5) and animals treated orally with 0.25 ml aqueous extracts of 10 g roots of Curcuma domestica once daily for 5 consecutive days (group-3, n = 5). All animals were anesthetized, and Sn-pyrophosphate and Tc99m solutions were injected intravenously for in vivo red cell radioactive labeling. Femoral arterial occlusion was observed, using a gamma camera. Induction of femoral arterial thrombosis was effected following an endothelial injury by free radicals produced by green light-irradiated Rose Bengal (10 mg/kg). The results showed that in the control group arterial total flow occlusion was seen in 15 min of irradiation. The results of MDA absorbency was 0.3700 +/- 1.7 nmol/ml in control group-1, 0.0520 +/- 0.025 in group-2 (significant p < 0.05 in comparison to control group) and 0.2780 +/- 0.027 in group-3 (non-significant). Interleukin-1alpha plasma level was 14.44 +/- 2.3 in control group-1, 8.93 +/- 2.4 in group-2 (significant p < 0.05), and 6.21 +/- 2.5 in group-3 (significant p < 0.05). Plasma thromboxane B2 plasma level was 20.31 +/- 2.4 in control group-1, 14.32 +/- 2.2 in group-2 (significant p < 0.05), and 19.41 +/- 2.1 in group-3 (significant). This study suggests the potential antioxidative, anti-inflammatory and antithrombotic effect that the dietary aqueous extracts has in rat femoral artery.
Rilantono
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Faculty of Medicine, University of Indonesia, National Cardiac Center, Jakarta. kardio@dnet.id
Yuwono
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Nugrahadi
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D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology* / Q000627:therapeutic use; D000975:Antioxidants / Q000494:pharmacology* / Q000627:therapeutic use; D001157:Arterial Occlusive Diseases / Q000000981:diagnostic imaging / Q000209:etiology / Q000517:prevention & control*; D030024:Curcuma; D004032:Diet; D004195:Disease Models, Animal; D005263:Femoral Artery; D005343:Fibrinolytic Agents / Q000494:pharmacology* / Q000627:therapeutic use; D007214:Indonesia; D007375:Interleukin-1 / Q000096:biosynthesis; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D010777:Photochemistry; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D010940:Plant Proteins / Q000494:pharmacology* / Q000627:therapeutic use; D011134:Polysaccharides / Q000494:pharmacology* / Q000627:therapeutic use; D011877:Radionuclide Imaging; D051381:Rats; D017208:Rats, Wistar; D012395:Rose Bengal / Q000528:radiation effects / Q000633:toxicity; D045730:Soy Foods; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis; D013929:Thromboxane B2 / Q000096:biosynthesis
D000894:Anti-Inflammatory Agents, Non-Steroidal; D000975:Antioxidants; D005343:Fibrinolytic Agents; D007375:Interleukin-1; D010936:Plant Extracts; D010940:Plant Proteins; D011134:Polysaccharides; D017392:Thiobarbituric Acid Reactive Substances; C412882:okara, Glycine max; D012395:Rose Bengal; D008315:Malondialdehyde; D013929:Thromboxane B2; C012596:turmeric extract