title: Assessment of the serodiagnostic potential of nine novel proteins from Mycobacterium tuberculosis.
abstract: To identify antigens that would improve the accuracy of serological diagnosis of active tuberculosis, we cloned the genes encoding nine potentially immunogenic secreted or surface-associated proteins of Mycobacterium tuberculosis. Recombinant proteins were reacted with sera from HIV-negative individuals with extrapulmonary tuberculosis (EP-TB) or HIV-positive individuals with pulmonary tuberculosis (TBH). Specific and high level antibody responses were obtained for four recombinant proteins, of which antigen GST-822 was recognized by 60% of EP-TB and 42% of TBH and antigen MBP-506 was recognized by 45% of EP-TB and 61% of TBH. These results suggest that these proteins are strong candidates as subunits in a polyvalent serodiagnostic test.
authors.affiliation: Department of Biochemistry and microbiology, University of Victoria, Victoria BC, Canada V8W 3P6, Brazil.
authors.forename: A J
authors.initials: AJ
authors.lastname: Moran
authors.forename: J D
authors.initials: JD
authors.lastname: Treit
authors.forename: J L
authors.initials: JL
authors.lastname: Whitney
authors.forename: B
authors.initials: B
authors.lastname: Abomoelak
authors.forename: R
authors.initials: R
authors.lastname: Houghton
authors.forename: Y A
authors.initials: YA
authors.lastname: Skeiky
authors.forename: D P
authors.initials: DP
authors.lastname: Sampaio
authors.forename: R
authors.initials: R
authors.lastname: Badaró
authors.forename: F E
authors.initials: FE
authors.lastname: Nano
mesh_terms: D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology*; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology; D003001:Cloning, Molecular; D004797:Enzyme-Linked Immunosorbent Assay; D005798:Genes, Bacterial; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000276:immunology*; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction; D011993:Recombinant Fusion Proteins / Q000276:immunology / Q000378:metabolism; D014376:Tuberculosis / Q000175:diagnosis*
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D011993:Recombinant Fusion Proteins