Impact of Celsior solution on hepatocellular, reperfusion and endothelial cell injury after liver transplantation.
BACKGROUND
Initially, preservation solutions were developed to maintain cell function of the transplanted organs. However, recently developed preservation solutions also contain a variety of substances to reduce the reperfusion injury.


OBJECTIVE
To study the effect of three different preservation solutions on the liver cell injury, endothelial cell function and reperfusion injury after liver transplantation.


METHODS
Large White X Landrace pigs of either sex were subjected to orthotopic liver transplantation. Donor livers were flushed and stored in University of Wisconsin Solution, Eurocollins Solution or Celsior Solution for 3 hours. Blood samples were taken at various times post transplantation for assessment of aspartate aminotransferase, hyaluronic acid, malondialdehyde and Vitamin A levels.


RESULTS
Serum aspartate aminotransferase levels were lower in the livers preserved in the Wisconsin solution. Plasma malondialdehyde levels were lower and serum Vitamin A levels were higher in the livers preserved in Celsior solution. Serum hyaluronic acid levels increased after liver transplantation but were similar with all three solutions.


CONCLUSIONS
There was less hepato-cellular injury in the livers preserved in Wisconsin solution and less reperfusion injury with the Celsior solution. The endothelial cell injury was similar with all three solutions.
van As
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Department of Surgery and the Medical Research Council, Liver Research Centre, University of Cape Town, South Africa. basvanas@iafrica.com
Lotz
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Tyler
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Kahn
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D000818:Animals; D001219:Aspartate Aminotransferases / Q000032:analysis; D004187:Disaccharides / Q000494:pharmacology*; D004195:Disease Models, Animal; D004573:Electrolytes / Q000494:pharmacology*; D004727:Endothelium / Q000166:cytology / Q000187:drug effects; D005260:Female; D005971:Glutamates / Q000494:pharmacology*; D005978:Glutathione / Q000494:pharmacology*; D006084:Graft Rejection; D006085:Graft Survival; D006639:Histidine / Q000494:pharmacology*; D006820:Hyaluronic Acid / Q000032:analysis; D016031:Liver Transplantation / Q000379:methods*; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D008353:Mannitol / Q000494:pharmacology*; D009926:Organ Preservation; D019188:Organ Preservation Solutions / Q000737:chemistry* / Q000494:pharmacology*; D011336:Probability; D015427:Reperfusion Injury / Q000517:prevention & control*; D012680:Sensitivity and Specificity; D013552:Swine; D014184:Transplantation, Homologous; D014801:Vitamin A / Q000032:analysis
C088453:Celsior; D004187:Disaccharides; D004573:Electrolytes; D005971:Glutamates; D019188:Organ Preservation Solutions; D014801:Vitamin A; D008353:Mannitol; D006639:Histidine; D008315:Malondialdehyde; D006820:Hyaluronic Acid; D001219:Aspartate Aminotransferases; D005978:Glutathione