title: Production of glucose oxidase using Aspergillus niger and corn steep liquor.
abstract: Glucose oxidase production was optimized using an isolated strain of Aspergillus niger and an economical nutrient source, corn steep liquor (CSL). The culture produced 580 +/- 30 units/ml of the enzyme using 70 g/l sucrose as the carbon source. Using CSL as the sole nutrient source enzyme synthesis was increased to 640 +/- 36 units/ml. None of the nitrogen sources (nitrates of calcium, sodium, ammonium, potassium and yeast extract, malt extract, and peptone) was beneficial to the enzyme synthesis. Aeration and agitation enhanced enzyme synthesis to 850 +/- 45 units/ml. Glucose oxidase has numerous applications in food industry and clinical fields.
authors.affiliation: Department of Chemical Technology, University of Bombay, Matunga, India.
authors.forename: R P
authors.initials: RP
authors.lastname: Kona
authors.forename: N
authors.initials: N
authors.lastname: Qureshi
authors.forename: J S
authors.initials: JS
authors.lastname: Pai
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology*; D019149:Bioreactors; D001709:Biotechnology; D002244:Carbon / Q000378:metabolism; D003470:Culture Media; D005285:Fermentation; D005949:Glucose Oxidase / Q000096:biosynthesis*; D009584:Nitrogen / Q000378:metabolism; D003313:Zea mays
chemical_list: D003470:Culture Media; D002244:Carbon; D005949:Glucose Oxidase; D009584:Nitrogen