title: Autologous blood transfusion as an immunomodulator in experimental sepsis.
abstract: BACKGROUND
  Homologous blood transfusion is associated with immunosuppressive consequences. Some clinical and experimental studies have suggested an immunostimulating action of autologous blood transfusion. The aim of this paper is to ascertain the effects of either homologous blood transfusion or autologous blood transfusion on the lymphocyte subsets and cytokines in a model of intra-abdominal sepsis.
  
  
  METHODS
  There were three study groups. Group A: 10 Wistar-Furth (WF) rats underwent cecal ligation and puncture (CLP) aimed at causing an intra-abdomial sepsis; Group B: 10 WF rats underwent CLP plus 1 ml homologous blood perioperative transfusion obtained from Fisher-344 rat while Group C: 10 WF rats underwent CLP plus 1 ml autologous blood perioperative transfusion. Changes of peripheral lymphocyte subsets, percentages of total T-lymphocytes (CD3), Helper T-lymphocytes (CD4), supressor/cytotoxic T-lymphocytes (CD8), CD4/CD8 ratio, Interleukin-2 receptor expression (IL-2R) and cytokines IL-1 and TNF-alpha were measured in peripheral blood on the preoperative, 1st, 3rd and 7th postsepsis (PO) days.
  
  
  RESULTS
  Rats in homologous transfused group showed a decrease of %CD4 on the 3rd PO (from preoperative to 3rd PO;p < 0.01; and from 1st to 3rd PO; p < 0.05) and on the 7th PO (from preoperative to 7th PO; p < 0.05); %CD8 increased from preoperative to 3rd PO (p < 0.05), from 1st to 3rd PO (p < 0.01) and from 1st to 7th PO (p < 0.05). An initial decrease on day 1 (p < 0.01) followed by an increase on the 3rd PO (p < 0.01) with regard to IL-2R and a significant increase of IL-1 levels within the first 24h (p < 0.01). Rats in autologous transfused group showed an increase of %CD3 from preoperative to 7th PO (p < 0.05), and from 3rd to 7th PO (p < 0.01).
  
  
  CONCLUSIONS
  We observed that homologous blood transfusions induce a greater alteration in the cellular immune response and of the cascade of cytokines than autologous transfusions. This modulates the variations of the immune response induced by sepsis.
authors.affiliation: Department of General and Thoracic Surgery, University Hospital Lozano Blesa, University of Zaragoza, Spain. soucur@comz.org
authors.forename: R
authors.initials: R
authors.lastname: Sousa
authors.forename: J C
authors.initials: JC
authors.lastname: Salinas
authors.forename: M
authors.initials: M
authors.lastname: Navarro
authors.forename: A
authors.initials: A
authors.lastname: Güemes
authors.forename: J
authors.initials: J
authors.lastname: Torcal
authors.forename: F
authors.initials: F
authors.lastname: García-Alvarez
authors.forename: R
authors.initials: R
authors.lastname: Cabezalí
authors.forename: L
authors.initials: L
authors.lastname: Larrad
authors.forename: R
authors.initials: R
authors.lastname: Lozano
mesh_terms: D000818:Animals; D001424:Bacterial Infections / Q000097:blood / Q000401:mortality / Q000628:therapy*; D001772:Blood Cell Count; D001804:Blood Transfusion, Autologous; D007114:Immunization; D007375:Interleukin-1 / Q000097:blood; D008297:Male; D051381:Rats; D011916:Rats, Inbred F344; D011920:Rats, Inbred WF; D015375:Receptors, Interleukin-2 / Q000097:blood; D016176:T-Lymphocyte Subsets / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
chemical_list: D007375:Interleukin-1; D015375:Receptors, Interleukin-2; D014409:Tumor Necrosis Factor-alpha