Survival and neurite outgrowth of rat cortical neurons in three-dimensional agarose and collagen gel matrices.
To better understand interactions between neurons and extracellular matrix equivalents, embryonic day-18 rat cortical neurons were immobilized and maintained in culture for up to 24 days in agarose and type I collagen gels. Using live/dead staining, neuronal cultures in low density collagen gel lasted at least 3 weeks. At 14 days, over 50% of immobilized cells in collagen gel were found viable while in low density agarose gel no cells survived. In situ cell death detection showed that most, if not all, dead cells in either of the gels underwent apoptosis. The collagen-trapped neurons exhibited normal neuronal polarity and developed long neurites, estimated at over 500 microm. The results suggest that collagen, because it is a major extracellular matrix constituent, suppresses apoptosis and provides a suitable substrate for neuronal survival and differentiation.
O'Connor
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SM
Center for Bio/Molecular Science and Engineering, Code 6910, Naval Research Laboratory, 4555 Overlook Avenue. S.W., Washington, D.C. 20375, USA.
Stenger
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DA
Shaffer
K M
KM
Ma
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W
D000818:Animals; D017209:Apoptosis; D016764:Cell Polarity; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology / Q000502:physiology*; D003094:Collagen / Q000494:pharmacology*; D003584:Cytological Techniques; D004622:Embryo, Mammalian; D005782:Gels; D016501:Neurites / Q000502:physiology*; D009474:Neurons / Q000502:physiology*; D051381:Rats; D012685:Sepharose / Q000494:pharmacology*; D013997:Time Factors
D005782:Gels; D003094:Collagen; D012685:Sepharose