Expression of canine interferon-gamma by a recombinant vaccinia virus and its antiviral effect.
A recombinant vaccinia virus-expressing canine interferon (IFN)-gamma (vv/cIFN-gamma) was constructed. In rabbit kidney (RK13) and canine A72 cells infected with vv/cIFN-gamma, IFN activity was detected in the culture supernatants of both cell types. Canine IFN-gamma was also detected in both cell extracts by Western blot. The activity of the recombinant canine IFN-gamma in RK13 cells was higher than that in A72 cells. The vv/cIFN-gamma could not grow in A72 cells at a low multiplicity of infection, probably due to the antiviral activity of the canine IFN-gamma produced. Although exogenous IFN-gamma did not inhibit the growth of vaccinia virus, addition of anti-canine IFN-gamma serum recovered the growth of the vv/cIFN-gamma on A72 cells in a dose-dependent manner. These results suggest that the growth of vv/cIFN-gamma was inhibited by IFN-gamma produced in a paracrine and autocrine manner. In addition, the recombinant canine IFN-gamma inhibited the multiplication of canine herpesvirus, pseudorabies virus and canine adenovirus type 1 in Madin-Darby canine kidney cells. The antiviral effect of canine IFN-gamma was more effective than that of canine IFN-beta. From the present studies, we concluded the recombinant virus may be a useful suicide viral vector.
Nishikawa
Y
Y
National Research Center for Protozoan Diseases, Obihiro University, Inadacho, Obihiro, 080-8555, Hokkaido, Japan.
Iwata
A
A
Katsumata
A
A
Xuan
X
X
Nagasawa
H
H
Igarashi
I
I
Fujisaki
K
K
Otsuka
H
H
Mikami
T
T
D000818:Animals; D000998:Antiviral Agents / Q000138:chemical synthesis / Q000378:metabolism* / Q000494:pharmacology; D002460:Cell Line; D004285:Dogs; D005822:Genetic Vectors / Q000494:pharmacology; D007371:Interferon-gamma / Q000096:biosynthesis* / Q000235:genetics* / Q000502:physiology; D009500:Neutralization Tests; D011817:Rabbits; D011995:Recombination, Genetic; D014616:Vaccinia virus / Q000235:genetics* / Q000254:growth & development
D000998:Antiviral Agents; D007371:Interferon-gamma