title: Fast repair of the radical cations of dCMP and poly C by quercetin and rutin.
abstract: The effects of quercetin and rutin on the repair of the radical cations of dCMP and poly C were studied using the technique of pulse radiolysis. The radical cations of dCMP and poly C were formed by the reaction of dCMP and poly C with SO( 4)(-). After pulse irradiation of nitrogen-saturated aqueous solutions containing dCMP, 20 mM K(2)S(2)O(8), 200 mM t-BuOH and either rutin or quercetin, the initially formed radical cation of dCMP, detected spectrophotometrically, rapidly decayed with the concurrent formation of the phenoxyl radical of rutin or quercetin within 8-40 micros. The repair efficiencies of the tested compounds towards the poly C radical cation were also determined using the same procedure. The results indicate that dCMP and poly C radical cations can be rapidly repaired by quercetin and rutin. The rate constants of the repair reactions were determined to be 4.3-8.8x10(8) M/s and 1.5-3.6x10(8) M/s for dCMP and poly C radical cations, respectively. Together with findings from our previous studies, the present results demonstrate that non-enzymatic fast repair may be a universal form of repair involving phenolic antioxidants.
authors.affiliation: School of Life Science, Lanzhou University, Lanzhou, 730000, China.
authors.forename: C
authors.initials: C
authors.lastname: Zhao
authors.forename: Y
authors.initials: Y
authors.lastname: Shi
authors.forename: W
authors.initials: W
authors.lastname: Lin
authors.forename: W
authors.initials: W
authors.lastname: Wang
authors.forename: Z
authors.initials: Z
authors.lastname: Jia
authors.forename: S
authors.initials: S
authors.lastname: Yao
authors.forename: B
authors.initials: B
authors.lastname: Fan
authors.forename: R
authors.initials: R
authors.lastname: Zheng
mesh_terms: D000975:Antioxidants / Q000494:pharmacology; D002412:Cations; D003843:Deoxycytidine Monophosphate / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D005419:Flavonoids / Q000378:metabolism; D008956:Models, Chemical; D011066:Poly C / Q000378:metabolism*; D011794:Quercetin / Q000494:pharmacology*; D012431:Rutin / Q000494:pharmacology*; D013053:Spectrophotometry; D013997:Time Factors
chemical_list: D000975:Antioxidants; D002412:Cations; D005419:Flavonoids; D003843:Deoxycytidine Monophosphate; D011066:Poly C; D012431:Rutin; D011794:Quercetin