title: A simple technique to concentrate the protozoan Mikrocytos mackini, causative agent of Denman Island Disease in oysters.
abstract: This report describes a simple filtration technique to isolate the parasite Mikrocytos mackini from oyster tissue. The technique is based on successive filtration through filter papers and polycarbonate membrane filters of decreasing mesh using a low vacuum (<8 cm Hg). This technique allows for the recovery of about 1 x 10(8) parasites (microcells) from about 2 g of heavily infected oyster tissue. About 99% of the particulate material in the final preparation consisted of intact M. mackini.
authors.affiliation: IFREMER, La Trinité-sur-Mer, France.
authors.forename: J P
authors.initials: JP
authors.lastname: Joly
authors.forename: S M
authors.initials: SM
authors.lastname: Bower
authors.forename: G R
authors.initials: GR
authors.lastname: Meyer
mesh_terms: D000818:Animals; D056890:Eukaryota / Q000302:isolation & purification*; D005374:Filtration / Q000379:methods; D010125:Ostreidae / Q000469:parasitology*; D010274:Parasitology / Q000379:methods*