Antagonistic effect of CCK-8 on morphine-inhibited electrical and contractile activities of rat jejunum in vitro.
In the present investigation, antagonistic action of cholecystokinin octapeptide (CCK-8) against morphine on the electrical and contractile activity of rat jejunum in vitro was studied. The results showed that the potentiation of acetylcholine (ACh) on both the burst of spike and the contractility were inhibited by morphine, which could be completely antagonized by CCK-8. The CCK-8 effect, again, could be suppressed by CCK-A receptor antagonist devazepide (10 nmol/L), but partially by CCK-B receptor antagonist L-365, 260 at 10 nmol/L or completely at concentration of 30 nmol/L. The above results demonstrated that the antagonism of CCK-8 on morphine was mediated by both CCK-A and CCK-B receptors.
Xu
M Y
MY
Department of Physiology, Harbin Medical University, Harbin 150086.
Yang
D X
DX
Wang
S Z
SZ
Jin
H B
HB
Zou
X H
XH
Yang
X P
XP
Han
J S
JS
D000818:Animals; D001570:Benzodiazepinones / Q000494:pharmacology; D020109:Devazepide / Q000494:pharmacology*; D004594:Electrophysiology; D005260:Female; D007583:Jejunum / Q000187:drug effects / Q000502:physiology*; D008297:Male; D009020:Morphine / Q000037:antagonists & inhibitors*; D009119:Muscle Contraction / Q000187:drug effects*; D009130:Muscle, Smooth / Q000187:drug effects / Q000502:physiology*; D010671:Phenylurea Compounds / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D043886:Receptor, Cholecystokinin A; D043887:Receptor, Cholecystokinin B; D011949:Receptors, Cholecystokinin / Q000037:antagonists & inhibitors; D012844:Sincalide / Q000494:pharmacology*
D001570:Benzodiazepinones; D010671:Phenylurea Compounds; D043886:Receptor, Cholecystokinin A; D043887:Receptor, Cholecystokinin B; D011949:Receptors, Cholecystokinin; C058121:L 365260; D009020:Morphine; D020109:Devazepide; D012844:Sincalide