Detection of Chlamydia trachomatis in semen and urethral specimens from male members of infertile couples in Tunisia.
The sequelae to infection with Chlamydia trachomatis in women are an established cause of tubal infertility. However, little is known about chlamydial infection and male infertility. The main objective of this study was to evaluate the presence of asymptomatic C. trachomatis infections in urethral and semen specimens from the male members of infertile couples by means of four different methods: the direct fluorescence antibodies assay, cell culture, the Roche Cobas Amplicor polymerase chain reaction, and the presence of chlamydial local IgA antibodies by the recombinant antibody-enzyme-linked immunosorbent assay. One or more chlamydial infection markers were detected in 42 (45.7%) of the 92 examined urethral and semen specimens from the male partners of infertile couples. C. trachomatis was detected in 23.9% (22/92) of urethral specimens and in 35.9% (33/92) of semen specimens. Although there was a significant correlation between the detection of one or more chlamydial infection markers in urethral and semen specimens (p = 0.01), no significant correlation was found between the detection of C. trachomatis DNA in these samples. Furthermore, no significant association was found between the presence of chlamydial local IgA antibodies and the detection of C. trachomatis. The discrepancies in positive results found between some techniques for the detection of C. trachomatis in urethral and semen specimens might be explained by variations in the sensitivities and specificities of the tests carried out and the use of specimens from different anatomical locations. Our findings suggest that C. trachomatis seems to be widespread among the male partners of infertile couples in Tunisia. The detection of C. trachomatis in urethral or semen specimens can serve as a marker for the presence of this organism in the genital tract, which is not necessarily the cause of male infertility. The study of the correlation between the detection of chlamydial infection markers and the parameters of male fertility seems to be necessary in order to determine the direct link between chlamydial infection and male infertility and to choose the most efficient technique and most suitable specimen with which to diagnose C. trachomatis-associated male infertility.
Gdoura
R
R
Laboratory of Bacteriology-Virology, University School of Medicine, Sfax, Tunisia.
Daoudi
F
F
Bouzid
F
F
Ben Salah
F
F
Chaigneau
C
C
Sueur
J M
JM
Eb
F
F
Rekik
S
S
Hammami
A
A
Orfila
J
J
D000328:Adult; D000914:Antibodies, Viral / Q000302:isolation & purification; D002692:Chlamydia trachomatis / Q000302:isolation & purification*; D004279:DNA, Viral / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007248:Infertility, Male / Q000821:virology*; D008297:Male; D008875:Middle Aged; D012661:Semen / Q000821:virology*; D014416:Tunisia; D014521:Urethra / Q000821:virology*
D000914:Antibodies, Viral; D004279:DNA, Viral