Indirect neurotrophic effect of neuropeptide ZNC(C)PR on PC12 cells via peptide-stimulation of C6 cells.
OBJECTIVE
To understand the mechanism of neurotrophic action of neuropeptide ZNC(C)PR and its effect on which could affect both growth and apoptosis of C6 cells.


METHODS
Effects of ZNC(C)PR-treated C6 conditioned medium was observed on on culture of PC12 cells. The development of PC12 cells was determined by ratio of neurite-bearing cells in the total cells. The specific binding of ZNC(C)PR on C6 cells was determined by radioligand binding assay (RBA).


RESULTS
ZNC(C)PR-treated C6 conditioned medium increased the ratio of neurite-bearing PC12 cells by 36% compared to the untreated C6 conditioned medium or to a mixture of ZNC(C)PR with the untreated C6 conditioned medium. RBA showed a specific binding site of ZNC(C)PR on C6 cells with Kd value of 2.74 nmol.L-1 and Bmax value of 19 pmol.g-1 protein.


CONCLUSIONS
ZNC(C)PR enhanced C6 cells induced secretion of some neurotrophic factors which acted as enhancers for PC12 cells differentiation, through its specific receptor sites on the neuroglioma cell.
Yan
Q W
QW
Shanghai Institute of Biochemistry, Chinese Academy of Sciences, Shanghai 200031, China.
Zhen
X G
XG
Wang
T X
TX
Xu
K Y
KY
Dong
M
M
Du
Y C
YC
D000818:Animals; D001127:Arginine Vasopressin / Q000494:pharmacology*; D001665:Binding Sites; D001932:Brain Neoplasms / Q000473:pathology; D002454:Cell Differentiation; D002455:Cell Division; D002462:Cell Membrane; D017077:Culture Media, Conditioned; D005910:Glioma / Q000473:pathology; D009414:Nerve Growth Factors / Q000494:pharmacology*; D016716:PC12 Cells; D010446:Peptide Fragments / Q000494:pharmacology*; D051381:Rats; D014407:Tumor Cells, Cultured
C094362:(phenylmethoxy)carbonyl-asparaginyl-(cysteinyl)cysteinyl-prolyl-arginine; D017077:Culture Media, Conditioned; D009414:Nerve Growth Factors; D010446:Peptide Fragments; D001127:Arginine Vasopressin