Thiophilic interaction chromatography of prostate-specific antigen.
Prostate-specific antigen (PSA) protein and complexes of PSA with alpha1-antichymotrypsin (PSA-ACT) or alpha2-macroglobulin (PSA-A2M) prepared in vitro, have strong affinity for different thiophilic gels (T-gel). Free PSA, and these PSA complexes can be isolated due to their affinity for T-gels. The average recovery of PSA from several of the T-gels, based upon ELISA measurements, was 84 to 94%. The data suggest that T-gel affinity can be explored for the purification of free and complexed PSA from various biologic fluids.
Chadha
K C
KC
Roswell Park Cancer Institute, Department of Molecular and Cellular Biology, Buffalo, NY 14263, USA. kailash.chadha@roswellpark.org
Kawinski
E
E
Sulkowski
E
E
D000906:Antibodies / Q000276:immunology; D015153:Blotting, Western; D002846:Chromatography, Affinity / Q000379:methods*; D005260:Female; D005782:Gels / Q000737:chemistry; D006801:Humans; D008297:Male; D017430:Prostate-Specific Antigen / Q000032:analysis* / Q000276:immunology
D000906:Antibodies; D005782:Gels; D017430:Prostate-Specific Antigen