Differences in arginine vasotocin gene transcripts and cortisol secretion in trout with high or low endogenous melanin-concentrating hormone secretion.
Previous studies on trout suggest that melanin-concentrating hormone (MCH) acts at both hypothalamic and pituitary levels to restrain the release of adrenocorticotropic hormone and hence cortisol during stress. Using in situ hybridization, the present work examined whether high rates of MCH secretion were associated with changes in the synthesis of arginine vasotocin (AVT), one of the corticotropin secretogogues. It also examined whether high endogenous MCH secretion restrains cortisol secretion during intense as well as mild stress, and how exogenous MCH affects the rise in plasma cortisol following injection stress. Trout were reared in black- or white-coloured tanks for 1 year or more to achieve maximal differences in MCH secretion. Following a mild stress, cortisol secretion was greater in black-reared fish with low MCH secretion which is in line with previous findings but, following a more severe stress, plasma cortisol concentrations were similar in the two groups. Injection of MCH into black-adapted fish restrained the stress-induced rise in plasma cortisol concentration during the first hour but did not affect final cortisol values. In two separate experiments, AVT mRNA levels were significantly lower in the hypothalamus of black-reared fish. Possible explanations for this include a greater negative-feedback restraint by cortisol, which is likely to rise higher in black-adapted fish during the moderate, daily stresses of aquarium life; or the possibility that exposure to a white background may be psychologically stressful, stimulating AVT transcription. The possibility that MCH directly stimulates AVT transcription cannot be excluded but seems less likely. The results suggest that while MCH may restrain the release of hypothalamo-pituitary stress hormones under moderately stressful conditions, it does not restrain AVT synthesis.
Gilchriest
B J
BJ
Department of Biology and Biochemistry, University of Bath, Bath, UK.
Tipping
D J
DJ
Hake
L
L
Levy
A
A
Baker
B I
BI
D000818:Animals; D003116:Color; D015870:Gene Expression / Q000502:physiology; D006799:Housing, Animal; D006854:Hydrocortisone / Q000097:blood / Q000378:metabolism*; D007028:Hypothalamic Hormones / Q000378:metabolism*; D017403:In Situ Hybridization; D008543:Melanins / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism; D017686:Oncorhynchus mykiss / Q000502:physiology*; D010907:Pituitary Hormones / Q000378:metabolism*; D011301:Preoptic Area / Q000166:cytology / Q000378:metabolism; D011333:Pro-Opiomelanocortin / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D013312:Stress, Physiological / Q000378:metabolism / Q000503:physiopathology; D014668:Vasotocin / Q000235:genetics*
D007028:Hypothalamic Hormones; D008543:Melanins; D010907:Pituitary Hormones; D012333:RNA, Messenger; D011333:Pro-Opiomelanocortin; C019463:melanin-concentrating hormone; D014668:Vasotocin; D006854:Hydrocortisone