Structural enzymology of biotin biosynthesis.
Over the last years, significant progress has been made in the understanding of the genetics and enzymology of the biosynthetic pathway of the vitamin biotin. The enzymes catalyzing the last four steps of this pathway, from pimeloyl-CoA to biotin, provide an ensemble of intriguing reaction mechanisms, which are presently being unravelled. The three-dimensional structures for three of these enzymes are known and provide a framework to which on-going mechanistic studies can be related.
Schneider
G
G
Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Scheelevägen 2, S-171 77, Stockholm, Sweden. gunter@alfa.mbb.ki.se.
Lindqvist
Y
Y
D000214:Acyl Coenzyme A / Q000378:metabolism; D000217:Acyltransferases / Q000378:metabolism; D000596:Amino Acids / Q000096:biosynthesis; D001710:Biotin / Q000096:biosynthesis*; D019731:Carbon-Nitrogen Ligases / Q000378:metabolism; D004798:Enzymes / Q000737:chemistry* / Q000378:metabolism*; D008958:Models, Molecular; D013329:Structure-Activity Relationship; D000637:Transaminases / Q000378:metabolism
D000214:Acyl Coenzyme A; D000596:Amino Acids; D004798:Enzymes; C019527:pimeloyl-coenzyme A; C001502:7-keto-8-aminopelargonic acid; D001710:Biotin; D000217:Acyltransferases; C021929:8-amino-7-oxononanoate synthase; C020029:7,8-diaminopelargonic acid aminotransferase; D000637:Transaminases; D019731:Carbon-Nitrogen Ligases; C020024:dethiobiotin synthetase