Binding features of chloroplast fructose-1,6-bisphosphatase-thioredoxin interaction.
It has been proposed that a hydrophobic groove surrounded by positively charged amino acids on thioredoxin (Trx) serves as the recognition and docking site for the interaction of Trx with target proteins. This model for Trx-protein interactions fits well with the Trx-mediated fructose-1,6-bisphosphatase (FBPase) activation, where a protruding negatively charged loop of FBPase would bind to this Trx groove, in a process involving both electrostatic and hydrophobic interactions. This model facilitates the prediction of Trx amino acid residues likely to be involved in enzyme binding. Site-directed mutagenesis of some of these amino acids, in conjunction with measurements of the FBPase activation capacity of the wild type and mutated Trxs, was used to check the model and provided evidence that lysine-70 and arginine-74 of pea Trx m play an essential role in FBPase binding. The binding parameters for the interaction between chloroplast FBPase and the wild type pea Trxs f and m, as well as mutated pea Trx m, determined by equilibrium dialysis in accordance with the Koshland-Nemethy-Filmer model of saturation kinetics, provided additional support for the role of these basic Trx residues in the interaction with FBPase. These data, in conjunction with the midpoint redox potential (E(m)) determinations of Trxs, support the hydrophobic groove model for the interaction between chloroplast FBPase and Trx. This model predicts that differences in the FBPase activation capacity of Trxs arise from their different binding abilities.
Wangensteen
O S
OS
Department of Biochemistry, Cell and Molecular Biology of Plants, Estación Experimental del Zaidin, Granada, Spain.
Chueca
A
A
Hirasawa
M
M
Sahrawy
M
M
Knaff
D B
DB
López Gorgé
J
J
D001120:Arginine / Q000737:chemistry; D001665:Binding Sites; D002736:Chloroplasts / Q000201:enzymology*; D004789:Enzyme Activation; D004926:Escherichia coli / Q000378:metabolism; D006597:Fructose-Bisphosphatase / Q000378:metabolism*; D005822:Genetic Vectors; D006863:Hydrogen-Ion Concentration; D008239:Lysine / Q000737:chemistry; D008962:Models, Theoretical; D009154:Mutation; D010084:Oxidation-Reduction; D018532:Pisum sativum; D011199:Potentiometry; D013879:Thioredoxins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
D013879:Thioredoxins; D001120:Arginine; D006597:Fructose-Bisphosphatase; D008239:Lysine