title: In vivo regulation of glutamine synthetase activity in the marine chlorophyll b-containing cyanobacterium Prochlorococcus sp. strain PCC 9511 (oxyphotobacteria).
abstract: The physiological regulation of glutamine synthetase (GS; EC 6.3.1.2) in the axenic Prochlorococcus sp. strain PCC 9511 was studied. GS activity and antigen concentration were measured using the transferase and biosynthetic assays and the electroimmunoassay, respectively. GS activity decreased when cells were subjected to nitrogen starvation or cultured with oxidized nitrogen sources, which proved to be nonusable for Prochlorococcus growth. The GS activity in cultures subjected to long-term phosphorus starvation was lower than that in equivalent nitrogen-starved cultures. Azaserine, an inhibitor of glutamate synthase, provoked an increase in enzymatic activity, suggesting that glutamine is not involved in GS regulation. Darkness did not affect GS activity significantly, while the addition of diuron provoked GS inactivation. GS protein determination showed that azaserine induces an increase in the concentration of the enzyme. The unusual responses to darkness and nitrogen starvation could reflect adaptation mechanisms of Prochlorococcus for coping with a light- and nutrient-limited environment.
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Universidad de Córdoba, E-14071 Córdoba, Spain.
authors.forename: S
authors.initials: S
authors.lastname: El Alaoui
authors.forename: J
authors.initials: J
authors.lastname: Diez
authors.forename: L
authors.initials: L
authors.lastname: Humanes
authors.forename: F
authors.initials: F
authors.lastname: Toribio
authors.forename: F
authors.initials: F
authors.lastname: Partensky
authors.forename: J M
authors.initials: JM
authors.lastname: García-Fernández
mesh_terms: D000222:Adaptation, Physiological; D002734:Chlorophyll / Q000378:metabolism*; D003470:Culture Media; D000458:Cyanobacteria / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism; D003624:Darkness; D005974:Glutamate-Ammonia Ligase / Q000235:genetics / Q000378:metabolism*; D015151:Immunoblotting / Q000379:methods; D008027:Light; D009584:Nitrogen / Q000378:metabolism*; D010788:Photosynthesis / Q000187:drug effects
chemical_list: D003470:Culture Media; D002734:Chlorophyll; C037184:chlorophyll b; D005974:Glutamate-Ammonia Ligase; D009584:Nitrogen