Bias-corrected confidence intervals for the concentration parameter in a dilution assay.
Interval estimates of the concentration of target entities from a serial dilution assay are usually based on the maximum likelihood estimator. The distribution of the maximum likelihood estimator is skewed to the right and is positively biased. This bias results in interval estimates that either provide inadequate coverage relative to the nominal level or yield excessively long intervals. Confidence intervals based on both log transformation and bias reduction are proposed and are shown through simulations to provide appropriate coverage with shorter widths than the commonly used intervals in a variety of designs. An application to feline AIDS research, which motivated this work, is also presented.
Wang
J
J
Center for Human Nutrition, University of Texas Southwestern Medical Center at Dallas, 75235-9052, USA. wang@crcdec.swmed.edu
Basu
S
S
D000163:Acquired Immunodeficiency Syndrome / Q000821:virology; D000818:Animals; D015982:Bias; D001699:Biometry; D002415:Cats; D016001:Confidence Intervals; D016181:Feline Acquired Immunodeficiency Syndrome / Q000821:virology; D006678:HIV / Q000302:isolation & purification; D006801:Humans; D016089:Immunodeficiency Virus, Feline / Q000302:isolation & purification; D007201:Indicator Dilution Techniques / Q000706:statistics & numerical data*; D016013:Likelihood Functions