Improved method for the measurement of large neutral amino acids in biological matrices.
A high-performance liquid chromatographic method for measuring neutral amino acids in rat sera, brain tissues, and perfusates was developed by using o-phthalaldehyde sulfite as a pre-column derivatization reagent. With the present method, it was possible to separate the neutral amino acids within a single run in 25 min, while the acidic amino acids were eluted near or at the solvent front. The recovery was above 88.8% with a relative standard deviation (RSD) below 4.2%. The within- and between-day assay reproducibility for the determination of rat serum amino acids showed RSDs below 1.35 and 7.61%, respectively. In the present study, the neutral amino acids were assayed with high sensitivity, accuracy and good reproducibility in a relatively short time and on a small sample size.
Bongiovanni
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R
Veterans Affairs Medical Center, GMH(B), Brecksville, OH 44141, USA. rbongi4333@aol.com
Yamamoto
B K
BK
Jaskiw
G E
GE
D000596:Amino Acids / Q000032:analysis / Q000097:blood*; D000818:Animals; D001921:Brain / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
D000596:Amino Acids