title: Impact of MK-886 on H2O2 generation by human neutrophils and cell degranulation.
abstract: Metabolic inhibitors can clearly affect different aspects of the functional activity of cells. This property was studied in the present work with respect to MK-886, a well-known inhibitor of the 5'-lipoxygenase-activating protein. It was found that this inhibitor in a micromolar concentration range (2-20 microM) induced in a dose-dependent manner H2O2 generation by human neutrophils and the release of lysozyme from the cells. The MK-886-induced activation of neutrophils was accompanied by a significant decrease in N-(1-pyrene)maleimide-accessible SH-groups in the cells. According to its activity, MK-886 can be considered an agonist that causes up-regulation of inherent neutrophil functions. In summary, the results indicate that during the application of MK-886 as a 5;-lipoxygenase inhibitor in neutrophils, the impact of the compound on the functional activity of the cells should be taken into consideration.
authors.affiliation: Belarusian State University, Minsk, 220050, Belarus. gorudko@phys.bsu.unibel.by
authors.forename: I V
authors.initials: IV
authors.lastname: Gorudko
authors.forename: A V
authors.initials: AV
authors.lastname: Timoshenko
mesh_terms: D015550:Cell Degranulation; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism*; D007211:Indoles / Q000494:pharmacology*; D016859:Lipoxygenase Inhibitors / Q000494:pharmacology*; D009113:Muramidase / Q000378:metabolism; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism*; D010084:Oxidation-Reduction; D013439:Sulfhydryl Reagents / Q000378:metabolism; D013997:Time Factors; D015854:Up-Regulation; D002722:Chlorobenzenes
chemical_list: D006861:Hydrogen Peroxide; D007211:Indoles; D016859:Lipoxygenase Inhibitors; D009113:Muramidase; D013439:Sulfhydryl Reagents; C060893:MK-886; D002722:Chlorobenzenes