Polar production of interleukin-8 by mesothelial cells promotes the transmesothelial migration of neutrophils: role of intercellular adhesion molecule-1.
Migration of polymorphonuclear neutrophils (PMNL) from the vascular compartment into the pleural space occurs rapidly during the development of parapneumonic effusions. This study investigated the polarized secretion of interleukin (IL)-8 in activated pleural mesothelial cells (PMC) and the migration of PMNL across resting, activated PMC monolayers. Results show that PMC produce IL-8 in a polar manner. When PMC were stimulated with Staphylococcus aureus or IL-1beta at the basal or at the apical surface, significantly (P< .05) more IL-8 was released toward the apical surface. This polarized production of IL-8 was confirmed by in situ hybridization. PMNL migration was higher from the basilar to apical than from the apical to basilar surface of PMC. Neutralizing antibodies against IL-8 and intercellular adhesion molecule (ICAM)-1 significantly (P< .001) blocked PMNL migration across activated monolayers. Thus, during pleural inflammation, PMC regulate the influx of PMNL into the pleural space by polar production of IL-8 and expression of ICAM-1.
Nasreen
N
N
Division of Pulmonary and Critical Care Medicine, Department of Veterans Affairs Medical Center, and Indiana University School of Medicine, Indianapolis, IN, USA.
Mohammed
K A
KA
Hardwick
J
J
Van Horn
R D
RD
Sanders
K L
KL
Doerschuk
C M
CM
Hott
J W
JW
Antony
V B
VB
D002465:Cell Movement; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000276:immunology / Q000382:microbiology; D004848:Epithelium / Q000276:immunology*; D005434:Flow Cytometry; D006801:Humans; D017403:In Situ Hybridization; D066298:In Vitro Techniques; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis / Q000502:physiology*; D016209:Interleukin-8 / Q000032:analysis / Q000494:pharmacology; D007963:Leukocytes, Mononuclear; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology*; D010994:Pleura / Q000166:cytology; D013211:Staphylococcus aureus
D016209:Interleukin-8; D018799:Intercellular Adhesion Molecule-1