Characterization of a novel carotenoid cleavage dioxygenase from plants.
The plant hormone abscisic acid is derived from the oxidative cleavage of a carotenoid precursor. Enzymes that catalyze this carotenoid cleavage reaction, nine-cis epoxy-carotenoid dioxygenases, have been identified in several plant species. Similar proteins, whose functions are not yet known, are present in diverse organisms. A putative cleavage enzyme from Arabidopsis thaliana contains several highly conserved motifs found in other carotenoid cleavage enzymes. However, the overall homology with known abscisic acid biosynthetic enzymes is low. To determine the biochemical function of this protein, it was expressed in Escherichia coli and used for in vitro assays. The recombinant protein was able to cleave a variety of carotenoids at the 9-10 and 9'-10' positions. In most instances, the enzyme cleaves the substrate symmetrically to produce a C(14) dialdehyde and two C(13) products, which vary depending on the carotenoid substrate. Based upon sequence similarity, orthologs of this gene are present throughout the plant kingdom. A similar protein in beans catalyzes the same reaction in vitro. The characterization of these activities offers the potential to synthesize a variety of interesting, natural products and is the first step in determining the function of this gene family in plants.
Schwartz
S H
SH
Department of Energy--Plant Research Laboratory, Michigan State University, East Lansing, MI 48824, USA.
Qin
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Zeevaart
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D000040:Abscisic Acid / Q000378:metabolism; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology*; D002851:Chromatography, High Pressure Liquid; D049308:Dioxygenases; D004926:Escherichia coli; D008956:Models, Chemical; D008969:Molecular Sequence Data; D010105:Oxygenases / Q000737:chemistry* / Q000235:genetics; D016415:Sequence Alignment
D000040:Abscisic Acid; D010105:Oxygenases; D049308:Dioxygenases; C431939:carotenoid cleavage dioxygenase 1