title: Separation of phenolic compounds by high-performance liquid chromatography with absorbance and fluorimetric detection.
abstract: Phenolic compounds including phenolic aldehydes, acids and flavonoids are separated by high-performance liquid chromatography (HPLC) with analysis time shorter than described in the literature. The use of a fluorescence detector in series with absorbance detector allowed increasing selectivity and sensitivity for the determination of catechin, vanillic acid, syringic acid, epicatechin and trans-resveratrol in wine samples. An optimised sample preparation method using liquid-liquid extraction with diethyl ether at pH 2.0 was used. The optimised method was applied to analyse wine samples with good results.
authors.affiliation: Department of Analytical Chemistry, Nutrition and Food Science, University of La Laguna, Tenerife, Spain. mrguez@ull.es
authors.forename: M A
authors.initials: MA
authors.lastname: Rodríguez-Delgado
authors.forename: S
authors.initials: S
authors.lastname: Malovaná
authors.forename: J P
authors.initials: JP
authors.lastname: Pérez
authors.forename: T
authors.initials: T
authors.lastname: Borges
authors.forename: F J
authors.initials: FJ
authors.lastname: García Montelongo
mesh_terms: D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D010636:Phenols / Q000302:isolation & purification*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
chemical_list: D010636:Phenols