title: [Removal of lepirudin used as an anticoagulant in mechanical autotransfusion with Cell-Saver 5].
abstract: Former studies demonstrated that small amounts of heparin might remain in the prepared retransfusion blood during intraoperative autotransfusion. This could lead to serious complications in patients suffering from heparin-induced-thrombocytopenia type II (HIT II). Lepirudin is an approved anticoagulant in HIT II-patients. We studied to what extent lepirudin is washed out during the preparation of retransfusion blood, when it is used as anticoagulant for the autotransfusion device cell saver 5.
  
  
  METHODS
  We investigated four different concentrations of lepirudin solutions, 5 mg, 10 mg, 20 mg and 30 mg per litre normal saline. In order to imitate a clinical situation, each lepirudin solution was mixed with human blood in a 1:5-ratio and put into the reservoir of the cell saver. The device was started in the automatic mode using 1000 ml saline as washing solution. Several runs were carried out (five times using the 5 mg/l solution, ten times the 10 mg/l, eleven times the 20 mg/l and eleven times the 30 mg/l solution). The lepirudin concentration in the prepared retransfusion blood was measured.
  
  
  RESULTS
  The median percentage reduction of the lepirudin content from the reservoir blood to the retransfusion blood was 100% for the 5 mg/l, 90.4% for the 10 mg/l, 94.3% for the 20 mg/l and 86.3% for the 30 mg/l solution. The differences of percentage reduction are not significant. But the different lepirudin concentrations in the anticoagulant solution have a significant influence on the lepirudin concentration in the retransfusion blood. The lepirudin concentration (median) in the retransfusion blood was 0.00 microgram/ml for the 5 mg/l, 0.16 microgram/ml for the 10 mg/l, 0.19 microgram/ml for the 20 mg/l and 0.66 microgram/l for the 30 mg/l lepirudin solution.
  
  
  CONCLUSIONS
  Lepirudin as an anticoagulant for intraoperative autotransfusion is effectively eliminated using the cell saver 5 device in the automatic mode with 1000 ml saline as washing solution. A clinically relevant disturbance of coagulation is not to be expected, even if the highest concentration of lepirudin anticoagulant solution investigated in this study is used.
authors.affiliation: Abteilung für Anästhesie und Intensivmedizin, Berufsgenossenschaftliche Unfallklinik, Frankfurt am Main.
authors.forename: A
authors.initials: A
authors.lastname: Marx
authors.forename: U
authors.initials: U
authors.lastname: von Lüpke
authors.forename: R
authors.initials: R
authors.lastname: Tessmann
authors.forename: E
authors.initials: E
authors.lastname: Lindhoff-Last
mesh_terms: D000925:Anticoagulants / Q000009:adverse effects / Q000097:blood* / Q000627:therapeutic use; D001804:Blood Transfusion, Autologous / Q000295:instrumentation / Q000379:methods*; D024764:Hirudin Therapy; D006629:Hirudins / Q000009:adverse effects / Q000031:analogs & derivatives* / Q000097:blood*; D006801:Humans; D007432:Intraoperative Period; D011994:Recombinant Proteins / Q000009:adverse effects / Q000097:blood* / Q000627:therapeutic use
chemical_list: D000925:Anticoagulants; D006629:Hirudins; D011994:Recombinant Proteins; C083544:lepirudin