title: [Distribution of dopamine D1 receptors in the paraventricular nucleus of the rat hypothalamus and their colocalization with phosphorylated forms of CREB protein].
abstract: The aim of the present immunohistochemical study was to investigate distribution of dopamine D1 receptors in the paraventricular nucleus of hypothalamus and to estimate whether D1 receptors colocalize with pCREB protein, a functional marker of stimulation generated via receptors positively linked to cAMP/PKA system. D1 receptors were found in numerous neurones of the paraventricular nucleus of hypothalamus, especially in its magnocellular part. In double-staining experiment, 74% of all stained neurones showed colocalization of D1 receptors and pCREB protein, 23% of neurones was pCREB-positive only and 3%--D1 receptor-positive only. The presence of D1 receptors in the paraventricular nucleus of hypothalamus indicates that these receptors may be possibly engaged in regulation of the endocrine system (release of oxytocin, vasopressin or CRH). Almost complete colocalization of D1 receptors with pCREB protein suggests that these receptors are likely functional (active). The presence of D1 receptors and pCREB protein in studied structures of rat brain, as well as the specificity of applied antibodies were confirmed by Western Blot method. It was demonstrated that antibodies against D1 receptor and pCREB protein recognized main bands with molecular weight approximately 40 and approximately 46 kDa, respectively, what correlates well with the literature data.
authors.affiliation: Zakład Farmakologii Instytut Farmakologii PAN ul. Smetna 12, 31-343 Kraków.
authors.forename: A
authors.initials: A
authors.lastname: Chocyk
authors.forename: K
authors.initials: K
authors.lastname: Szumierz
authors.forename: K
authors.initials: K
authors.lastname: Wedzony
authors.forename: A
authors.initials: A
authors.lastname: Czyrak
mesh_terms: D000818:Animals; D015153:Blotting, Western; D017362:Cyclic AMP Response Element-Binding Protein / Q000032:analysis* / Q000737:chemistry; D007150:Immunohistochemistry; D008297:Male; D008970:Molecular Weight; D010286:Paraventricular Hypothalamic Nucleus / Q000737:chemistry* / Q000166:cytology; D010766:Phosphorylation; D051381:Rats; D017208:Rats, Wistar; D017447:Receptors, Dopamine D1 / Q000032:analysis*
chemical_list: D017362:Cyclic AMP Response Element-Binding Protein; D017447:Receptors, Dopamine D1