Circulating bone marrow cells can contribute to neointimal formation.
To examine the source of smooth muscle-like cells during vascular healing, C57BL/6 (Ly 5.2) female mice underwent whole body irradiation followed by transfusion with 10(6) nucleated bone marrow cells from congenic (Ly 5.1) male donors. Successful repopulation (88.4 +/- 4.9%) by donor marrow was demonstrated in the female mice by flow cytometry with FITC-conjugated A20.1/Ly 5.1 monoclonal antibody after 4 weeks. The arteries of the female mice were then subjected to two types of insult: (1) The iliac artery was scratch-injured by 5 passes of a probe causing severe medial damage. After 4 weeks, the arterial lumen was obliterated by a cell-rich neointima, with cells containing alpha smooth muscle actin present around the residual lumen. Approximately half of these cells were of male donor origin, as evidenced by in situ hybridization with a Y-chromosome-specific probe. (2) In an organized arterial thrombus formed by inserting an 8-0 silk suture into the left common carotid artery, donor cells staining with alpha smooth muscle actin were found in those arteries sustaining serious damage but not in arteries with minimal damage. Our results suggest that bone marrow-derived cells are recruited in vascular healing as a complementary source of smooth muscle-like cells when the media is severely damaged and few resident smooth muscle cells are available to effect repair.
Han
C I
CI
Centre for Research in Vascular Biology, University of Queensland, Brisbane, Australia.
Campbell
G R
GR
Campbell
J H
JH
D000818:Animals; D001854:Bone Marrow Cells / Q000473:pathology*; D016026:Bone Marrow Transplantation; D020212:Carotid Artery Injuries / Q000473:pathology* / Q000503:physiopathology; D005260:Female; D005434:Flow Cytometry; D017403:In Situ Hybridization; D008297:Male; D051379:Mice; D020297:Mice, Congenic; D008810:Mice, Inbred C57BL; D009131:Muscle, Smooth, Vascular / Q000473:pathology*; D011828:Radiation Chimera; D017154:Stromal Cells / Q000473:pathology*; D013927:Thrombosis / Q000473:pathology / Q000503:physiopathology; D017539:Tunica Intima / Q000473:pathology; D014945:Wound Healing; D014998:Y Chromosome