title: Effect of anti-digoxin antiserum on endoxin and membrane ATPase activity in hypoxia-reoxygenation induced myocardial injury.
abstract: OBJECTIVE
  To evaluate the protective effect of anti-digoxin antiserum on hypoxia-reoxygenation induced injured myocardium and its mechanism.
  
  
  METHODS
  Anti-digoxin antiserum of different concentrations was used, its effect on endoxin and ATPase activity in cell membrane in hypoxia-reoxygenation myocardium model was observed.
  
  
  RESULTS
  The level of endoxin was remarkably higher, ATPase activities in cell membrane were remarkably lower in hypoxic group and hypoxia-reoxygenation injury group than those of normal group; anti-digoxin antiserum could resume ATPase activity in a concentration-dependent manner.
  
  
  CONCLUSIONS
  Rise of endoxin was the molecular biological basis of myocardial damage during myocardial hypoxia-reoxygenation. Anti-digoxin antiserum had lessened myocardial injury and had a protective effect on hypoxia-reoxygenation myocardium by antagonizing effect of endoxin.
authors.affiliation: Yijishan Hospital, Wannan Medical College, Wuhu 241001, China. editorys@mail.ahwhptt.net.cn
authors.forename: Y S
authors.initials: YS
authors.lastname: Ke
authors.forename: Z F
authors.initials: ZF
authors.lastname: Liu
authors.forename: H
authors.initials: H
authors.lastname: Yang
authors.forename: T
authors.initials: T
authors.lastname: Yang
authors.forename: J S
authors.initials: JS
authors.lastname: Huang
authors.forename: S B
authors.initials: SB
authors.lastname: Rui
authors.forename: G H
authors.initials: GH
authors.lastname: Cheng
authors.forename: Y X
authors.initials: YX
authors.lastname: Wang
mesh_terms: D000818:Animals; D000252:Calcium-Transporting ATPases / Q000378:metabolism*; D002298:Cardenolides; D015687:Cell Hypoxia; D002462:Cell Membrane / Q000378:metabolism; D004077:Digoxin / Q000276:immunology*; D004791:Enzyme Inhibitors / Q000378:metabolism; D005260:Female; D007106:Immune Sera / Q000494:pharmacology*; D008297:Male; D015428:Myocardial Reperfusion Injury / Q000378:metabolism; D009206:Myocardium / Q000166:cytology*; D011817:Rabbits; D012503:Saponins / Q000378:metabolism*
chemical_list: D002298:Cardenolides; D004791:Enzyme Inhibitors; D007106:Immune Sera; D012503:Saponins; C034504:digoxin-like factors; D004077:Digoxin; D000252:Calcium-Transporting ATPases