title: [Determination of morphine in compound liquorice tablets by high performance liquid chromatography with SEP-PAK C18 cartridge pretreatment].
abstract: An efficient method combining SEP-PAK C18 cartridge solid phase extraction (SPE) with reversed-phase high performance liquid chromatography for the quantitation of morphine in the Compound Liquorice Tablet is presented. The tablets have been used for making expectoration easy and relieving cough for years. The tablet powder (approximately 1 tablet) was added into a stoppered centrifuge tube, and vertically extracted with 5 mL of 0.5% HAc for 5 min. After centrifugation the supernatant was transferred to a beaker and the extraction was repeated twice with 4 mL and then 3 mL of 0.5% HAc. Five millilitres of carbonate buffer (pH 8.9) was added to the combined extracts. A SEP-PAK C18 cartridge was pretreated by passing methanol and distilled water, using a glass syringe. The mixture was applied on it and allowed to flow through. The cartridge was washed first with 5 mL of 10% methanol solution and then the morphine was eluted with 4 mL of 70% methanol solution into a 5 mL volumetric flask and was finally diluted to volume with 70% methanol solution. Analysis was performed on a mu-Bondapak C18 column(300 mm x 4.6 mm i.d., 10 microns) with 0.1 mol/L NaH2PO4-methanol(5:1) as the mobile phase and detection at 286 nm. The average recovery of morphine was (101.2 +/- 1.5)% and RSD was 1.5%. The method is simple, rapid, accurate, and reproducible, and can be used in drug control.
authors.affiliation: Fujian Institute for Drug Control, Fuzhou, 350001.
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.forename: J
authors.initials: J
authors.lastname: Zeng
mesh_terms: D000996:Antitussive Agents / Q000737:chemistry*; D004339:Drug Compounding; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005100:Expectorants / Q000737:chemistry*; D009020:Morphine / Q000032:analysis*; D013607:Tablets
chemical_list: D000996:Antitussive Agents; D004365:Drugs, Chinese Herbal; D005100:Expectorants; D013607:Tablets; D009020:Morphine