Human septin 3 on chromosome 22q13.2 is upregulated by neuronal differentiation.
An expression sequence tag identified in a screen for genes upregulated by retinoic acid induced neuronal differentiation of the human teratocarcinoma cell line Ntera2/D1 was found in close genomic proximity to a region of high sequence homology to the septin subfamily of GTPase genes. We could show that the tag corresponds to the 3' untranslated region of this novel gene named septin 3 and cloned three isoforms A (2191 bp), B (4378 bp), and C (1896 bp) from human Ntera2/D1 cDNA. We present the genomic localization and organization on chromosome 22q13.2, a chromosomal hot spot for translocations implicated in leukemia. Interestingly, MSF the closest paralog of septin 3 is a fusion partner in a therapy-related acute myeloid leukemia. Quantitative PCR confirmed the upregulation of the putative septin by neuronal differentiation and northern blotting showed only one band corresponding to sep3B with a neurospecific expression pattern in adult human tissues.
Methner
A
A
Department of Neurology and Zentrum für Molekulare Neurobiologie, University Hospital Hamburg, Falkenried 94, Hamburg, D-20251, Germany. methner@uke.uni-hamburg.de
Leypoldt
F
F
Joost
P
P
Lewerenz
J
J
D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics; D002892:Chromosomes, Human, Pair 22 / Q000235:genetics*; D003001:Cloning, Molecular; D020558:GTP Phosphohydrolases / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D009928:Organ Specificity; D010802:Phylogeny; D020161:Physical Chromosome Mapping; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D058112:Septins; D017386:Sequence Homology, Amino Acid; D018243:Teratocarcinoma / Q000235:genetics / Q000378:metabolism; D014212:Tretinoin / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D009419:Nerve Tissue Proteins; D020033:Protein Isoforms; D014212:Tretinoin; D020558:GTP Phosphohydrolases; D058112:Septins; C407343:septin 3