title: An improved method for the rapid preparation of 2-amino-4,4a-dihydro-4a,7-dimethyl-3H-phenoxazine-3-one, a novel antitumor agent.
abstract: A simple and rapid preparation method for a novel antitumor agent, 2-amino-4,4a-dihydro-4a,7-dimethyl-3H-phenoxazine-3-one (Phx) was described. The procedure included (1) the reaction of bovine hemolysates with 2-amino-5-methylphenol, (2) one-shot denaturation of hemoglobin and proteins by methanol, and removal of the denatured hemoglobin and proteins, (3) concentration of the reaction products, and (4) purification by a Sephadex column. These procedures yielded Phx in 34% yield.
authors.affiliation: Department of Biochemistry, Tokyo Medical University, Japan. tomoda@tokyo-med.ac.jp
authors.forename: A
authors.initials: A
authors.lastname: Tomoda
authors.forename: S
authors.initials: S
authors.lastname: Arai
authors.forename: R
authors.initials: R
authors.lastname: Ishida
authors.forename: T
authors.initials: T
authors.lastname: Shimamoto
authors.forename: K
authors.initials: K
authors.lastname: Ohyashiki
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000138:chemical synthesis* / Q000494:pharmacology*; D002417:Cattle; D003408:Cresols / Q000138:chemical synthesis*; D004247:DNA / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D018922:HL-60 Cells; D006454:Hemoglobins / Q000737:chemistry* / Q000187:drug effects; D006801:Humans; D000432:Methanol / Q000737:chemistry / Q000494:pharmacology; D010078:Oxazines / Q000138:chemical synthesis* / Q000494:pharmacology*; D011489:Protein Denaturation / Q000187:drug effects; D011506:Proteins / Q000737:chemistry / Q000187:drug effects
chemical_list: C104202:2-amino-4,4alpha-dihydro-4alpha,7-dimethyl-3H-phenoxazine-3-one; D000970:Antineoplastic Agents; D003408:Cresols; D006454:Hemoglobins; D010078:Oxazines; D011506:Proteins; D004247:DNA; C077618:2-amino-5-methylphenol; D000432:Methanol