title: Cell-free entry of human T-cell leukemia virus type 1 to mouse cells.
abstract: Human T-cell leukemia virus type 1 (HTLV-1) is the etiologic agent for adult T-cell leukemia and HTLV-1-associated myelopathy / tropical spastic paraparesis. Recently we infected newborn mice by inoculating HTLV-1-producing human cells, and found that T-cells, B-cells and granulocytes were infected in vivo. To understand the mechanism of viral-cell interaction and the pathogenesis of HTLV-1 using the mouse model, it is important to clarify the cellular tropism using a cell-free HTLV-1 transmission system. We employed a highly transmissible cell-free HTLV-1 produced by a feline kidney cell line, c77, and studied the susceptibility of 9 kinds of mouse cell lines, EL4, RLm1, CTLL-2, J774.1, DA-1, Ba / F3, WEHI-3, NIH3T3 and B1, and two kinds of human cell lines, Molt-4 and Hut78. HTLV-1 proviral sequence was found by PCR in all 9 mouse cell lines as well as in 2 human cell lines and viral entry was blocked with sera from an HTLV-1 carrier and an adult T-cell leukemia patient. Unexpectedly, mouse cell lines EL4 and RLm1 and human cell lines Molt-4 and Hut78 showed similar efficiency for viral entry. These results suggest a wide distribution of HTLV-1 receptor in mouse cells.
authors.affiliation: Department of Biochemistry and Molecular Oncology, Institute of Basic Medical Sciences and Center for Tsukuba Advanced Research Alliance, University of Tsukuba, Tsukuba, Ibaraki 305-8575, Japan.
authors.forename: R
authors.initials: R
authors.lastname: Feng
authors.forename: A
authors.initials: A
authors.lastname: Kabayama
authors.forename: K
authors.initials: K
authors.lastname: Uchida
authors.forename: H
authors.initials: H
authors.lastname: Hoshino
authors.forename: M
authors.initials: M
authors.lastname: Miwa
mesh_terms: D016475:3T3 Cells / Q000821:virology; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D002353:Carrier State / Q000097:blood / Q000276:immunology / Q000821:virology; D002415:Cats; D002460:Cell Line; D002474:Cell-Free System; D004279:DNA, Viral / Q000032:analysis; D015683:Gene Products, gag / Q000096:biosynthesis; D015368:Human T-lymphotropic virus 1 / Q000235:genetics / Q000276:immunology / Q000472:pathogenicity*; D006801:Humans; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000097:blood / Q000276:immunology / Q000821:virology; D051379:Mice; D016133:Polymerase Chain Reaction; D015682:Retroviridae Proteins, Oncogenic / Q000096:biosynthesis; D013601:T-Lymphocytes / Q000821:virology; D014771:Virion / Q000472:pathogenicity; D054301:gag Gene Products, Human Immunodeficiency Virus
chemical_list: D000914:Antibodies, Viral; D004279:DNA, Viral; D015683:Gene Products, gag; D015682:Retroviridae Proteins, Oncogenic; D054301:gag Gene Products, Human Immunodeficiency Virus; C060955:p19 protein, Human T-lymphotropic virus 1