Differential expression of cytokines and cytokine receptors in renal cell carcinoma.
Modulation of immunomechanisms by tumor cells can be caused by secretion of cytokines. In vitro data are usually gained in culture systems. It is debatable whether these systems are representative of the conditions inside the respective tumor tissues. Immunohistochemical studies of tumor tissue cryostats were compared with those in matched primary renal cell carcinoma tumor cell cultures. Results were correlated with histopathological characteristics and the in vitro cytotoxic effect of autologous tumor infiltrating lymphocytes (TILs). Expression of all studied cytokines and cytokine receptors could be shown in cryostats and cell cultures, but the detection pattern varied individually. The immunohistochemical results in cryostats were in good accordance with those in cell cultures. Expression of TGFbeta1 both in cryostats and cell cultures significantly correlated with the lack of cytotoxic activity of autologous TILs. Representative data can be obtained in tumor culture systems of primary renal cell carcinoma. TGFbeta1 secretion could play an important role in the interactions between tumor and cytotoxic cells.
Steiner
T
T
Department of Urology, Friedrich Schiller University, Jena, Germany. thomas.steiner@med.uni-jena.de
Junker
U
U
Salamon
A
A
Nuske
K
K
Schubert
J
J
D002292:Carcinoma, Renal Cell / Q000276:immunology* / Q000378:metabolism; D016207:Cytokines / Q000096:biosynthesis*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007680:Kidney Neoplasms / Q000276:immunology* / Q000378:metabolism; D018121:Receptors, Cytokine / Q000096:biosynthesis*; D014407:Tumor Cells, Cultured
D016207:Cytokines; D018121:Receptors, Cytokine