Retinol supplementation induces oxidative stress and modulates antioxidant enzyme activities in rat sertoli cells.
Recent intervention studies revealed that supplementation with retinoids resulted in a higher incidence of lung cancer. Recently the causal mechanism has begun to be clarified. We report here that retinol caused cellular oxidative stress and modulated superoxide dismutase, catalase and glutathione peroxidase activities. Retinol (7 microM) significantly increased TBARS, conjugated dienes, and hydroperoxide-initiated chemiluminescence in cultured Sertoli cells. In response to retinol treatment superoxide dismutase, catalase and glutathione peroxidase activities increased. TBARS content and catalase activities were decreased by a free radical scavenger. These findings suggest that retinol may induce oxidative stress and modulate antioxidant enzyme activities in Sertoli cells.
Dal-Pizzol
F
F
Laboratório de Estresse Oxidativo, Departamento de Bioquímica, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
Klamt
F
F
Benfato
M S
MS
Bernard
E A
EA
Moreira
J C
JC
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D002374:Catalase / Q000187:drug effects* / Q000494:pharmacology; D018929:Cell Culture Techniques; D016166:Free Radical Scavengers; D005979:Glutathione Peroxidase / Q000187:drug effects* / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species; D012708:Sertoli Cells / Q000187:drug effects* / Q000378:metabolism; D013482:Superoxide Dismutase / Q000187:drug effects* / Q000494:pharmacology; D017392:Thiobarbituric Acid Reactive Substances; D014801:Vitamin A / Q000494:pharmacology*
D000975:Antioxidants; D016166:Free Radical Scavengers; D017382:Reactive Oxygen Species; D017392:Thiobarbituric Acid Reactive Substances; D014801:Vitamin A; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase