title: Escherichia coli strains from edema disease: O serogroups, and genes for Shiga toxin, enterotoxins, and F18 fimbriae.
abstract: The objectives of the research were to determine the presence of the gene sequences for Shiga Toxin 2e (Stx2e), enterotoxins (ST-I, ST-II and LT-I), and F18 fimbriae in 144 Escherichia coli strains isolated from pigs with edema disease; to assess the ability of stx2e(+) strains to produce Stx2e; and to determine the O serogroups of the E. coli strains. Presence of the genes was determined by polymerase chain reaction (PCR), production of Stx2e was assessed by cytotoxicity for Vero and Hela cells, O serogroups were identified by agglutination with specific antisera. Of the 144 strains tested, 99 were stx2e(+) by PCR, but only 45 of these were Stx2e(+) in the cell culture assays. Among the 99 stx2e(+) strains, PCR detected the genes for F18ab, ST-I, ST-II, LT-I in 76, 40, 31 and 16 strains, respectively. Forty-one of the 99 sxt2e(+) strains belonged to O group 139; the rest did not belong to the classical edema disease O serogroups. It is likely that the enterotoxins, whose genes were detected at high frequency, are responsible for diarrhea seem in pigs with edema disease in Brazil.
authors.affiliation: Departamento de Microbiologia e Imunologia -- IB, Universidade Estadual de Campinas, P.O. Box 6109, 13081-970 Campinas, SP, Brazil.
authors.forename: A S
authors.initials: AS
authors.lastname: da Silva
authors.forename: G F
authors.initials: GF
authors.lastname: Valadares
authors.forename: M P
authors.initials: MP
authors.lastname: Penatti
authors.forename: B G
authors.initials: BG
authors.lastname: Brito
authors.forename: D
authors.initials: D
authors.lastname: da Silva Leite
mesh_terms: D000372:Agglutination Tests; D000818:Animals; D001938:Brazil; D002522:Chlorocebus aethiops; D004488:Edema Disease of Swine / Q000382:microbiology*; D004926:Escherichia coli / Q000145:classification / Q000235:genetics*; D004927:Escherichia coli Infections / Q000382:microbiology / Q000662:veterinary*; D010861:Fimbriae, Bacterial / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D019081:O Antigens / Q000097:blood*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D022641:Shiga Toxin 2 / Q000235:genetics; D022603:Shiga Toxins / Q000032:analysis / Q000235:genetics*; D014709:Vero Cells
chemical_list: D019081:O Antigens; D022641:Shiga Toxin 2; D022603:Shiga Toxins