[Study on effect of ABA, PEG4000 and AgNO3 on number of somatic embryos of Larix Principis-Rupprechtii by 311-A regression design].
This paper, the effects of ABA, PEG400 and AgNO3 on the number of somatic embryos of Larix Principis-Rupprechtii were carried out by 311-A regression design. The regression equation was established which expresses the number of somatic embryos of Larix Principis-Rupprechtii as function, and the ABA, PEG400 and AgNO3 on as variable respectively. Using the regression equation, the single factor effect and mutual effect between the number of somatic embryos of Larix Principis-Rupprechtii and ABA, PEG400 and AgNO3 was studied; the optimum concentration recipes can be obtained by computer processing, that are ABA: 18.9138 mg/L, PEG400: 88.8007 g/L and AgNO3: 10.7513 mg/L, the target somatic embryos number is 107.5278 each gram callus. The results of experiment showed that this method is simple, practical and rapid for selecting several hormone category and concentration recipes media of conifer somatic embryogenesis.
Qi
L W
LW
Molecular Genetic Department, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China. lwqi@rif.forestry.ac.cn
Han
Y F
YF
Li
L
L
Ewald
D
D
Han
S Y
SY
D000040:Abscisic Acid / Q000494:pharmacology*; D010944:Plants / Q000196:embryology*; D011092:Polyethylene Glycols / Q000494:pharmacology*; D012044:Regression Analysis; D012835:Silver Nitrate / Q000494:pharmacology*
D011092:Polyethylene Glycols; D000040:Abscisic Acid; D012835:Silver Nitrate