[Experimental study on erythrocyte of obstructive jaundice patient: its changes of membrane protein and mechanical properties].
The component of the erythrocyte membrane protein under three states obstruction, after surgical relief of obstruction, and normal) were analyzed qualitatively by using SDS-PAGE and the method of thin scanning. The result revealed that the membrane protein band 4.2 was absent under the state of obstruction and it recovered after surgical relief of obstruction. Then the mechanical properties of the above three cases of erythrocytes were tested by the micropipette aspiration system and the data were analyzed by Voigt model. The result showed that the elastic modulus and the coefficient of viscosity of the erythrocytes under the state of obstruction were greater than those of the normal; thus the deformability decreased, but "overcorrection" appeared after surgical relief of obstruction. The possible relations between the mechanical properties and the change of membrane protein were discussed.
Yang
R
R
Bioengineering Research Institute, Chongqin University, 630044.
Wu
Y
Y
Jiang
J
J
Wu
Z
Z
Long
M
M
Wu
Y
Y
D000328:Adult; D000368:Aged; D001457:Anion Exchange Protein 1, Erythrocyte / Q000378:metabolism; D002779:Cholestasis / Q000097:blood*; D004548:Elasticity; D004907:Erythrocyte Deformability; D004910:Erythrocyte Membrane / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism*; D008875:Middle Aged
D001457:Anion Exchange Protein 1, Erythrocyte; D008565:Membrane Proteins