[The protective effect of removing circulatory TNF by immuno-adsorption on endotoxin-induced acute lung injury].
OBJECTIVE
To study the protective effect of removing circulatory TNF by immunoadsorption on endotoxin-induced acute lung injury in rabbits.


METHODS
New Zealand white rabbits injected with lethal dose of endotoxin underwent hemoperfusion with immunoadsorbent. TNF and IL-8 levels in BALF were measured, the number of leukocytes and percentage of PMN in BALF were analyzed and the ratio of wet lung to body weight and pulmonary microvascular permeability index were also studied. In addition, the survival rate was observed.


RESULTS
Treatment with immunoadsorption markedly attenuated the pulmonary microvascular permeability increased by endotoxin and improved the survival rate.


CONCLUSIONS
Immunoadsorption to remove circulating TNF is an effective way to protect rabbits against endotoxin-induced acute lung injury.
Tang
B
B
Department of Nephrology, Nanfang Hospital, Guangzhou 510515.
Zhang
X
X
Hou
F
F
D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D005260:Female; D006464:Hemoperfusion / Q000379:methods; D007163:Immunosorbent Techniques; D016209:Interleukin-8 / Q000378:metabolism; D008070:Lipopolysaccharides; D008297:Male; D011817:Rabbits; D011897:Random Allocation; D012128:Respiratory Distress Syndrome / Q000097:blood / Q000139:chemically induced / Q000628:therapy*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D016209:Interleukin-8; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha