title: Variation of pH-measurement in platelet concentrates.
abstract: To measure pH in platelet concentrates, blood gas analysers with different calibration principles may be used. In this study, variances observed in pH measurements with two types of blood gas analysers were investigated. pH was measured in crystalloid solutions (platelet additive solution (PAS-II), phosphate-buffered solutions) and two types of platelet concentrates (containing 100% plasma, or 65% PAS-II/35% plasma) with two blood gas analysers: either using liquid and gas calibration (AVL 945), or only liquid calibration (AVL OMNI). These measurements were compared with a reference method. Especially for PAS-II, large variation in pH was observed between AVL 945, AVL OMNI and the reference method: 6.91 +/- 0.02, 7.35 +/- 0.02 and 7.188 +/- 0.010, respectively (mean +/- SD; n = 12, P < 0.0001, paired t-test). A significant difference in pH was also found for platelet concentrates in 65% PAS/35% plasma (6.88 +/- 0.09 on AVL 945 and 7.02 +/- 0.09 on AVL OMNI, n = 134, P < 0.0001). Comparison with the reference method revealed minor differences with AVL 945, whereas AVL OMNI gave a mean difference in pH of + 0.17. Platelets in 100% plasma revealed smaller differences (6.93 +/- 0.13 for AVL 945 and 6.99 +/- 0.13 for AVL OMNI, n = 95, P < 0.0001). We conclude that different blood gas analysers can yield different pH values, especially in weak buffered solutions such as platelet concentrates in PAS-II. Validation of blood gas analysers for pH measurement of these solutions is therefore mandatory.
authors.affiliation: Blood Bank North Holland, Amsterdam, The Netherlands. bnhams@hetnet.nl
authors.forename: P F
authors.initials: PF
authors.lastname: van der Meer
authors.forename: A P
authors.initials: AP
authors.lastname: van Zanten
authors.forename: R N
authors.initials: RN
authors.lastname: Pietersz
authors.forename: H W
authors.initials: HW
authors.lastname: Reesink
mesh_terms: D001784:Blood Gas Analysis / Q000295:instrumentation / Q000379:methods; D001792:Blood Platelets / Q000166:cytology / Q000502:physiology*; D002021:Buffers; D002138:Calibration; D002469:Cell Separation / Q000379:methods; D006801:Humans; D006863:Hydrogen-Ion Concentration; D017713:Platelet Transfusion
chemical_list: D002021:Buffers