title: Heparin inhibits plaque formation by cell-free Marek's disease viruses in vitro.
abstract: The mechanisms of Marek's disease virus (MDV) entry to host cells have not yet been analyzed. Heparan sulfate (HS) on the cell surface serves as a receptor for several herpesviruses in mammalian species. In this study, we demonstrated that plaque formation by cell-free MDV is inhibited by the addition of soluble heparin to the cell culture. Moreover, pretreatment of susceptible cells, chicken embryo fibroblasts, with heparinase, partially reduced infectivity of the cell-free MDV. From these results, it was suggested that the MDV entry, at least in the case of cell-free MDV, is dependent on the presence of cell surface glycosaminoglycans, principally HS.
authors.affiliation: Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
authors.forename: S I
authors.initials: SI
authors.lastname: Lee
authors.forename: K
authors.initials: K
authors.lastname: Ohashi
authors.forename: C
authors.initials: C
authors.lastname: Sugimoto
authors.forename: M
authors.initials: M
authors.lastname: Onuma
mesh_terms: D000818:Animals; D002642:Chick Embryo; D002645:Chickens; D004279:DNA, Viral / Q000737:chemistry / Q000302:isolation & purification; D006493:Heparin / Q000494:pharmacology* / Q000502:physiology; D019763:Heparin Lyase / Q000494:pharmacology; D006497:Heparan Sulfate / Q000378:metabolism / Q000502:physiology; D008381:Herpesvirus 2, Gallid / Q000235:genetics / Q000254:growth & development* / Q000472:pathogenicity; D008380:Marek Disease / Q000473:pathology / Q000821:virology*; D016133:Polymerase Chain Reaction / Q000662:veterinary
chemical_list: D004279:DNA, Viral; D006493:Heparin; D019763:Heparin Lyase; D006497:Heparan Sulfate