Intrapulmonary pharmacokinetics of ofloxacin in drug-resistant tuberculosis.
OBJECTIVE
To determine the lung epithelial lining fluid (ELF) and serum levels of ofloxacin in drug-resistant tuberculosis patients during treatment.


METHODS
Ten drug-resistant tuberculosis patients treated with ofloxacin containing regimens for at least 2 weeks were enrolled in the study. Subjects ingested ofloxacin 10 mg/kg and other anti-tuberculosis agents after overnight fasting.


RESULTS
Serum and bronchoalveolar lavage fluid were collected at 4 hours after treatment and assayed by high performance liquid chromatography. The mean concentrations of ofloxacin in serum and ELF were 5.889 +/- 1.096 and 16.583 +/- 8.697 mg/L, respectively. The mean ratio of ELF-to-serum ofloxacin concentration was 2.825 +/- 1.275.


CONCLUSIONS
Ofloxacin can penetrate well into the intra-alveolar fluid of patients treated for drug-resistant tuberculosis. The lung ELF concentrations were consistently higher than the minimal inhibitory concentrations of Mycobacterium tuberculosis as determined in vitro.
Chierakul
N
N
Division of Respiratory Disease and Tuberculosis, Department of Medicine, Faculty of Medicine, Siriraj Hospital, Mahidol University, 10700 Bangkok, Thailand. chana2508@hotmail.com
Klomsawat
D
D
Chulavatnatol
S
S
Chindavijak
B
B
D000328:Adult; D000890:Anti-Infective Agents / Q000493:pharmacokinetics* / Q000627:therapeutic use; D000995:Antitubercular Agents / Q000493:pharmacokinetics / Q000627:therapeutic use*; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D008168:Lung / Q000378:metabolism*; D008297:Male; D015242:Ofloxacin / Q000493:pharmacokinetics* / Q000627:therapeutic use; D018088:Tuberculosis, Multidrug-Resistant / Q000188:drug therapy / Q000378:metabolism*; D014397:Tuberculosis, Pulmonary / Q000188:drug therapy / Q000378:metabolism*
D000890:Anti-Infective Agents; D000995:Antitubercular Agents; D015242:Ofloxacin