[Role of metallothionein in the resistance of rat osteoblasts to zinc excess and deficiency].
This study is expected to investigate the role of metallothionein(MT) in the resistance of osteoblasts to high or low zinc conditions. Antisense MT oligonucleotide was used to reduce the content of MT in osteoblast. Cellular MT content was determined by silver/hemolysate saturation method, and the cytotoxicity was measured by the activity of LDH releasing from damaged cells. The results showed that antisense MT oligonucleotide markedly decreased the resistance of osteoblast to zinc excess and zinc deficiency. It suggested that MT played an important role in the modulation and maintenance of intracellular zinc homeostasis of osteoblasts.
Cen
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National Safety Assessment Center for Traditional Chinese Medicine, First University Hospital, West China University of Medical Sciences, Chengdu 610041, China.
Zhao
Y
Y
Wang
L
L
Wang
R
R
D000818:Animals; D006706:Homeostasis; D008668:Metallothionein / Q000502:physiology*; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D010006:Osteoblasts / Q000378:metabolism*; D051381:Rats; D015032:Zinc / Q000378:metabolism*
D016376:Oligonucleotides, Antisense; D008668:Metallothionein; D015032:Zinc