Trophic factor secreting kidney cell lines: in vitro characterization and functional effects following transplantation in ischemic rats.
Several kidney cell lines were investigated for their ability to produce glial cell line-derived neurotrophic factor (GDNF). Cell line-conditioned medium was analyzed using ELISA and two cell lines were identified which produce GDNF in physiologically active concentrations. ELISA analyses revealed that conditioned medium from these two cell lines also contained PDGF, bFGF, TGFbeta1 and TGFbeta2. Both of these cell lines were then transplanted into the striatal penumbra of rats, 1 h following middle cerebral artery occlusion. Behavioral testing revealed that both cell lines reduced the deficit associated with cerebral ischemia and reduced the infarct volume relative to controls. Reduction of infarct volume was likely achieved by the action of GDNF and/or other growth factors produced by the cells.
Johnston
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RE
Development and Plasticity Section, Cellular Neurobiology Research Branch, National Institute on Drug Abuse, 5500 Nathan Shock Drive, Baltimore, MD 21224, USA. rjohnsto@intra.nida.nih.gov
Dillon-Carter
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Freed
W J
WJ
Borlongan
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D000818:Animals; D001522:Behavior, Animal; D002545:Brain Ischemia / Q000523:psychology / Q000601:surgery*; D002460:Cell Line; D017690:Cell Transplantation; D002544:Cerebral Infarction / Q000473:pathology / Q000523:psychology / Q000601:surgery; D004797:Enzyme-Linked Immunosorbent Assay; D016332:Fetal Tissue Transplantation; D005333:Fetus; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism; D051100:Glial Cell Line-Derived Neurotrophic Factor; D006801:Humans; D007668:Kidney / Q000166:cytology* / Q000378:metabolism*; D008297:Male; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D010982:Platelet-Derived Growth Factor / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D016212:Transforming Growth Factor beta / Q000378:metabolism
C495039:GDNF protein, human; C495041:Gdnf protein, rat; D051100:Glial Cell Line-Derived Neurotrophic Factor; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D010982:Platelet-Derived Growth Factor; D016212:Transforming Growth Factor beta; D016222:Fibroblast Growth Factor 2