title: Experimental deer-to-deer transmission of Mycobacterium bovis.
abstract: OBJECTIVE
  To determine whether Mycobacterium bovis can be transmitted from experimentally infected deer to uninfected in-contact deer.
  
  
  METHODS
  Twenty-three 6-month-old white-tailed deer.
  
  
  METHODS
  On day 0, M bovis (2 X 10(8) colony-forming units) was administered by intratonsillar instillation to 8 deer; 3 control deer received saline (0.9% NaCl) solution. Eight in-contact deer were comingled with inoculated deer from day 21. On day 120, inoculated deer were euthanatized and necropsied. On day 180, 4 in-contact deer were euthanatized, and 4 new in-contact deer were introduced. On day 360, all in-contact deer were euthanatized. Rectal, oral, and nasal swab specimens and samples of hay, pelleted feed, water, and feces were collected for bacteriologic culture. Tissue specimens were also collected at necropsy for bacteriologic culture and histologic analysis.
  
  
  RESULTS
  On day 90, inoculated and in-contact deer developed delayed-type hypersensitivity (DTH) reactions to purified protein derivative of M bovis. Similarly, new in-contact deer developed DTH reactions by 100 days of contact with original in-contact deer. Tuberculous lesions in in-contact deer were most commonly detected in lungs and tracheobronchial and medial retropharyngeal lymph nodes. Mycobacterium bovis was isolated from nasal secretions and saliva from inoculated and in-contact deer, urine and feces from in-contact deer, and hay and pelleted feed.
  
  
  CONCLUSIONS
  Mycobacterium bovis is efficiently transmitted from experimentally infected deer to uninfected in-contact deer through nasal secretions, saliva, or contaminated feed. Wildlife management practices that result in unnatural gatherings of deer may enhance both direct and indirect transmission of M bovis.
authors.affiliation: National Animal Diseases Center, Agricultural Research Service, USDA, Ames, IA 50010, USA.
authors.forename: M V
authors.initials: MV
authors.lastname: Palmer
authors.forename: D L
authors.initials: DL
authors.lastname: Whipple
authors.forename: W R
authors.initials: WR
authors.lastname: Waters
mesh_terms: D000821:Animal Feed / Q000382:microbiology; D000818:Animals; D003670:Deer / Q000382:microbiology*; D005243:Feces / Q000382:microbiology; D005260:Female; D007668:Kidney / Q000382:microbiology; D008168:Lung / Q000382:microbiology; D008198:Lymph Nodes / Q000382:microbiology; D008297:Male; D009163:Mycobacterium bovis / Q000254:growth & development / Q000302:isolation & purification; D011897:Random Allocation; D014132:Trachea / Q000382:microbiology; D014376:Tuberculosis / Q000473:pathology / Q000635:transmission / Q000662:veterinary*; D014871:Water Microbiology