Inhibitory effect of glycation on catalytic activity of alanine aminotransferase.
Non-enzymatic glycation is a common post-translational modification of tissue and plasma proteins which can impair their functions in living organisms. In this study, the authors have demonstrated for the first time an inhibitory effect of in vitro glycation on the catalytic activity of alanine aminotransferase (ALT, EC 2.6.1.2), a pyridoxal phosphate enzyme with several lysine residues in the molecule. The porcine heart enzyme was incubated with 50 mmol/l D-fructose, D-glucose, D,L-glyceraldehyde, or D-ribose in 0.1 mol/l phosphate buffer (pH 7.4) at 25 degrees C for up to 20 days. The strongest glycation effect was shown by D,L-glyceraldehyde, which caused complete enzyme inhibition within 6 days. After 20 days of incubation, the ALT activity in samples with D-fructose and D-ribose was less than 7% of the initial enzyme activity. A statistically significant effect of D-glucose on the enzymatic activity of ALT was not found. Incubation of ALT with D-fructose, D,L-glyceraldehyde and D-ribose minimized its catalytic activity both in the glycated and non-glycated fractions of the samples. Markedly higher activity was found in the glycated fraction with glucose. The inhibitory effect of glycation of ALT with D-fructose and D-ribose was found to be more intensive in the presence of L-alanine and weaker in the presence of 2-oxoglutarate. The findings suggest that glycation of the epsilon-amino group of Lys313 as a crucial part of the catalytic site of ALT may contribute to ALT inactivation in the presence of glycating sugars. Nevertheless, glycation of lysine residues outside the active center of ALT seems to be primary.
Beránek
M
M
Institute of Clinical Biochemistry and Diagnostics, Faculty Hospital, Hradec Králové, Czech Republic.
Drsata
J
J
Palicka
V
V
D000409:Alanine / Q000037:antagonists & inhibitors; D000410:Alanine Transaminase / Q000378:metabolism*; D000818:Animals; D002384:Catalysis; D004789:Enzyme Activation / Q000187:drug effects; D005632:Fructose / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D005985:Glyceraldehyde / Q000037:antagonists & inhibitors; D066298:In Vitro Techniques; D007656:Ketoglutaric Acids / Q000378:metabolism*; D008239:Lysine / Q000378:metabolism; D009206:Myocardium / Q000201:enzymology; D010600:Pharmacology; D011732:Pyridoxal Phosphate; D012266:Ribose / Q000378:metabolism*; D013552:Swine; D013997:Time Factors
D007656:Ketoglutaric Acids; D005632:Fructose; D005985:Glyceraldehyde; D011732:Pyridoxal Phosphate; D012266:Ribose; D000410:Alanine Transaminase; D005947:Glucose; D008239:Lysine; D000409:Alanine