title: Effect of PMMA particles and movement on an implant interface in a canine model.
abstract: The pathogenesis of aseptic loosening of total joint prostheses is not clearly understood. Two features are associated with loosened prostheses, namely, particulate debris and movement of the implant. While numerous studies have evaluated the cellular response to particulate biomaterials, few have investigated the influence of movement of the implant on the biological response to particles. Our aim was therefore to test the hypothesis that excessive mechanical stimulation of the periprosthetic tissues induces an inflammatory response and that the addition of particulate biomaterials intensifies this. We allocated 66 adult Beagle dogs to four groups as follows: stable implants with (I) and without (II) particulate polymethylmethacrylate (PMMA) and moving implants with (III) and without (IV) particulate PMMA. They were then evaluated at 2, 4, 6, 12 and 24 weeks. The stable implants were well tolerated and a thin, fibrous membrane of connective tissue was observed. There was evidence of positive staining in some cells for interleukin-6 (IL-6). Addition of particulate PMMA around the stable implants resulted in an increase in the fibroblastic response and positive staining for IL-6 and tumour necrosis factor-alpha (TNF-alpha). By contrast, movement of the implant resulted in an immediate inflammatory response characterised by large numbers of histiocytes and cytokine staining for IL-1beta, TNF-alpha and IL-6. Introduction of particulate PMMA aggravated this response. Animals with particulate PMMA and movement of the implant have an intense inflammatory response associated with accelerated bone loss. Our results indicate that the initiation of the inflammatory response to biomaterial particles was much slower than that to gross mechanical instability. Furthermore, when there was both particulate debris and movement, there was an amplification of the adverse tissue response as evidenced by the presence of osteolysis and increases in the presence of inflammatory cells and their associated cytokines.
authors.affiliation: Department of Orthopaedic Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland 21239, USA.
authors.forename: L C
authors.initials: LC
authors.lastname: Jones
authors.forename: C
authors.initials: C
authors.lastname: Frondoza
authors.forename: D S
authors.initials: DS
authors.lastname: Hungerford
mesh_terms: D000818:Animals; D004285:Dogs; D005260:Female; D006644:Histiocytes / Q000473:pathology; D007150:Immunohistochemistry; D007249:Inflammation / Q000209:etiology; D007375:Interleukin-1 / Q000032:analysis; D015850:Interleukin-6 / Q000032:analysis; D007595:Joint Prosthesis; D008297:Male; D009068:Movement; D010014:Osteolysis / Q000209:etiology; D019904:Polymethyl Methacrylate / Q000009:adverse effects*; D011475:Prosthesis Failure; D014409:Tumor Necrosis Factor-alpha
chemical_list: D007375:Interleukin-1; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D019904:Polymethyl Methacrylate