title: Solution structure of a C-terminal coiled-coil domain from bovine IF(1): the inhibitor protein of F(1) ATPase.
abstract: Bovine IF(1) is a basic, 84 amino acid residue protein that inhibits the hydrolytic action of the F(1)F(0) ATP synthase in mitochondria under anaerobic conditions. Its oligomerization state is dependent on pH. At a pH value below 6.5 it forms an active dimer. At higher pH values, two dimers associate to form an inactive tetramer. Here, we present the solution structure of a C-terminal fragment of IF(1) (44-84) containing all five of the histidine residues present in the sequence. Most unusually, the molecule forms an anti-parallel coiled-coil in which three of the five histidine residues occupy key positions at the dimer interface.
authors.affiliation: MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 2QH, UK.
authors.forename: D J
authors.initials: DJ
authors.lastname: Gordon-Smith
authors.forename: R J
authors.initials: RJ
authors.lastname: Carbajo
authors.forename: J C
authors.initials: JC
authors.lastname: Yang
authors.forename: H
authors.initials: H
authors.lastname: Videler
authors.forename: M J
authors.initials: MJ
authors.lastname: Runswick
authors.forename: J E
authors.initials: JE
authors.lastname: Walker
authors.forename: D
authors.initials: D
authors.lastname: Neuhaus
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002417:Cattle; D019281:Dimerization; D006639:Histidine / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008958:Models, Molecular; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D020836:Protein Structure, Quaternary; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism; D006180:Proton-Translocating ATPases / Q000037:antagonists & inhibitors*; D012996:Solutions; D013816:Thermodynamics; D000097795:ATPase Inhibitory Protein
chemical_list: D006639:Histidine; D010446:Peptide Fragments; D011506:Proteins; D006180:Proton-Translocating ATPases; D012996:Solutions; D000097795:ATPase Inhibitory Protein