[Presynaptic effects of botulinum toxin type A on the neuronally evoked response of albino and pigmented rabbit iris sphincter and dilator muscles].
OBJECTIVE
To investigate the effects of botulinum toxin type A(botulinum A toxin) on the autonomic and other non-adrenergic, non-cholinergic nerve terminals.


METHODS
The effects of neurotoxin on twitch contractions evoked by electrical field stimulation (EFS) were studied in isolated rabbit iris sphincter and dilator muscles using isometric tension recording.


RESULTS
Botulinum A toxin(150 nM) inhibited the fast cholinergic and slow substance P-ergic component of contraction evoked by EFS in the rabbit iris sphincter muscle without affecting the response to carbachol and substance P. Botulinum A toxin(150 nM) did not affect the twitch contraction evoked by EFS in the rabbit iris dilator muscle.


CONCLUSIONS
These data indicated that botulinum A toxin may inhibit not only the acetylcholine release in the cholinergic nerve terminals, but also substance P release from the trigeminal nerve terminals of the rabbit iris sphincter muscle. However, neurotoxin has little effect on the adrenergic nerve terminals of the rabbit iris dilator muscle.
Ishikawa
H
H
Department of Ophthalmology Kitasato University, School of Medicine, 1-15-1 Kitasato, Sagamihara, 228-8555, Japan.
Mitsui
Y
Y
Yoshitomi
T
T
Mashimo
K
K
Aoki
S
S
Mukuno
K
K
Shimizu
K
K
D000818:Animals; D019274:Botulinum Toxins, Type A / Q000494:pharmacology*; D066298:In Vitro Techniques; D007498:Iris / Q000187:drug effects*; D008297:Male; D009132:Muscles / Q000187:drug effects; D017729:Presynaptic Terminals / Q000187:drug effects*; D011817:Rabbits
D019274:Botulinum Toxins, Type A