title: Tissue distribution and temperature-dependence of Anguilla anguilla L. EROD activity following exposure to model inducers and relationship with plasma cortisol, lactate and glucose levels.
abstract: Anguilla anguilla L. ethoxyresorufin-O-deethylase (EROD) elevation by 2.7 microM beta-naphthoflavone (BNF) 3 days water exposure, or 4 mg/kg ip exposure, was studied in four different organs--liver, kidney, gills, and intestine. The results demonstrated a significant increase in liver EROD activity for the two previous conditions, whereas kidney EROD activity only increased during the intraperitoneal exposure. A. anguilla was also exposed during 8, 16, and 24 h to water contaminated with 2.7 microM BNF or benzo[a]pyrene (BaP) at 20 degrees C and 25 degrees C. Both compounds significantly increased liver EROD activity from 8 up to 24 h. There was no significant difference in liver EROD activity elevation by both compounds, either at 20 degrees C or 25 degrees C. Liver EROD activity was demonstrated to be one of the first warning systems concerning the presence of polycyclic aromatic hydrocarbons (PAHs) in water. A. anguilla 3 h exposure to diesel oil water-soluble fraction (DWSF) significantly increased plasma cortisol and significantly decreased plasma lactate. A prolonged exposure beyond 3 h, i.e. 4 h, 2, 3, 4, and 6 days to the previous conditions demonstrated a significant liver EROD activity elevation from Day 2 up to 6, and a significant increase in erythrocytic nuclear abnormalities (ENA) at Day 6.
authors.affiliation: Biology Department, University of Aveiro, Portugal. mpacheco@bio.ua.pt
authors.forename: M
authors.initials: M
authors.lastname: Pacheco
authors.forename: M A
authors.initials: MA
authors.lastname: Santos
mesh_terms: D000818:Animals; D001564:Benzo(a)pyrene / Q000493:pharmacokinetics*; D001786:Blood Glucose; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism*; D004781:Environmental Exposure; D005399:Fishes / Q000378:metabolism*; D006854:Hydrocortisone / Q000097:blood; D019344:Lactic Acid / Q000097:blood; D013696:Temperature; D014874:Water Pollutants, Chemical / Q000493:pharmacokinetics*; D019324:beta-Naphthoflavone / Q000493:pharmacokinetics*
chemical_list: D001786:Blood Glucose; D014874:Water Pollutants, Chemical; D019344:Lactic Acid; D001564:Benzo(a)pyrene; D019324:beta-Naphthoflavone; D019363:Cytochrome P-450 CYP1A1; D006854:Hydrocortisone