title: Potentiation of baroreceptor reflex response by heat shock protein 70 in nucleus tractus solitarii confers cardiovascular protection during heatstroke.
abstract: BACKGROUND
  Whereas hypotension and bradycardia seen during the onset of heatstroke may be protected by prior induction of heat shock protein 70 (HSP70) in the brain, the underlying mechanism is not fully understood. We evaluated the hypothesis that HSP70 may confer cardiovascular protection during heatstroke by potentiating the baroreceptor reflex (BRR) control of peripheral hemodynamic performance.
  
  
  RESULTS
  Adult male Sprague-Dawley rats subjected to a brief hyperthermic heat shock (HS; 42 degrees C for 15 minutes) induced discernible expression of HSP70 in the bilateral nucleus tractus solitarii (NTS), the terminal site in the brain stem for primary baroreceptor afferents. This HSP70 expression was detected at 8 hours, peaked at 24 hours, and returned to baseline by 48 hours after HS. Brief hyperthermia also significantly potentiated the BRR response in a temporal profile that correlated positively with changes in HSP70 expression at the NTS. Prior HS also appreciably alleviated hyperthermia, severe hypotension, and bradycardia manifested during the onset of heatstroke (45 degrees C for 60 minutes) elicited 24 hours later. Microinjection bilaterally of anti-HSP70 antiserum (1:20) into the NTS or denervation of the sinoaortic baroreceptor afferents significantly reversed the enhancement of BRR response and cardiovascular protection during heatstroke induced by prior HS.
  
  
  CONCLUSIONS
  These results suggest that HS-induced expression of HSP70 in the NTS may alleviate severe hypotension and bradycardia exhibited during the onset of heatstroke by potentiating both the sensitivity and capacity of BRR response.
authors.affiliation: Department of Biological Science and Center for Neuroscience, National Sun Yat-sen University, Taiwan, Republic of China.
authors.forename: P L
authors.initials: PL
authors.lastname: Li
authors.forename: Y M
authors.initials: YM
authors.lastname: Chao
authors.forename: S H
authors.initials: SH
authors.lastname: Chan
authors.forename: J Y
authors.initials: JY
authors.lastname: Chan
mesh_terms: D000818:Animals; D017704:Baroreflex / Q000502:physiology; D001831:Body Temperature / Q000187:drug effects; D001919:Bradycardia / Q000188:drug therapy / Q000209:etiology / Q000503:physiopathology; D002319:Cardiovascular System / Q000503:physiopathology*; D018840:HSP70 Heat-Shock Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D006339:Heart Rate / Q000187:drug effects; D018883:Heat Stroke / Q000150:complications / Q000503:physiopathology*; D006979:Hyperthermia, Induced; D007022:Hypotension / Q000188:drug therapy / Q000209:etiology / Q000503:physiopathology; D007106:Immune Sera / Q000008:administration & dosage; D008297:Male; D008845:Microinjections; D051381:Rats; D017207:Rats, Sprague-Dawley; D017552:Solitary Nucleus / Q000187:drug effects / Q000503:physiopathology*; D013562:Sympathectomy; D014666:Vasomotor System / Q000503:physiopathology
chemical_list: D018840:HSP70 Heat-Shock Proteins; D007106:Immune Sera