title: Localization of the netrin guidance receptor, DCC, in the developing peripheral and enteric nervous systems.
abstract: Over recent years the secreted guidance cue, netrin-1, and its receptor, DCC, have been shown to be an essential guidance system driving axon pathfinding within the developing vertebrate central nervous system (CNS). Mice lacking DCC exhibit severe defects in commissural axon extension towards the floor plate demonstrating that the DCC-netrin guidance system is largely responsible for directing axonal projections toward the ventral midline in the developing spinal cord (Fazeli et al., Nature 386 (1997) 796). In addition, these mutants lack several major commissures within the forebrain, including the corpus callosum and the hippocampal commissure. In contrast to the CNS, the role of the DCC guidance receptor in the development of the mammalian peripheral and enteric nervous systems (PNS and ENS) has not been investigated. Here we demonstrate using immunohistochemical analysis that the DCC receptor is present in the developing mouse PNS where it is found on spinal, segmental, and sciatic nerves, and in developing sensory ganglia and their associated axonal projections. In addition, DCC is present in the ENS throughout the early developmental phase.
authors.affiliation: Development and Neurobiology Unit, Walter and Eliza Hall Institute, Post Office, Royal Melbourne Hospital, Melbourne, Victoria 3050, Australia.
authors.forename: C
authors.initials: C
authors.lastname: Seaman
authors.forename: R
authors.initials: R
authors.lastname: Anderson
authors.forename: B
authors.initials: B
authors.lastname: Emery
authors.forename: H M
authors.initials: HM
authors.lastname: Cooper
mesh_terms: D000818:Animals; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D003337:Corpus Callosum / Q000196:embryology; D000075389:DCC Receptor; D006624:Hippocampus / Q000196:embryology; D007150:Immunohistochemistry; D051379:Mice; D009414:Nerve Growth Factors / Q000378:metabolism*; D000075388:Netrin-1; D017933:Peripheral Nervous System / Q000196:embryology*; D011956:Receptors, Cell Surface; D013116:Spinal Cord / Q000196:embryology; D013270:Stomach / Q000196:embryology* / Q000294:innervation*; D013997:Time Factors; D014018:Tissue Distribution; D025521:Tumor Suppressor Proteins
chemical_list: D015815:Cell Adhesion Molecules; D000075389:DCC Receptor; C501910:Dcc protein, mouse; D009414:Nerve Growth Factors; C000617353:Ntn1 protein, mouse; D011956:Receptors, Cell Surface; D025521:Tumor Suppressor Proteins; D000075388:Netrin-1