Cytochrome P450 1A1 and 4A activities in isolated rat spleen lymphocytes.
In this study the activity of Cytochrome P 450 (CYP) enzymes in isolated spleen lymphocytes of Wistar rats was estimated. 7-Ethoxyresorufin-O-deethylase was analysed to determine the activity of CYP 1A1/2. The activity was increased 2.2-fold by pretreating the animals with 3-methylcholanthrene, and 1.3-fold after the addition of the mitogen concanavalin A to the lymphocyte culture. Pretreatment of the rats with phenobarbitone did not enhance the basal enzyme activity. To estimate reductive activities the azoreduction of 4-(N,N-dimethyl-amino)azobenzene was analysed. Again detectable turnovers were found which could be increased 4-fold after the addition of concanavalin A. Azo-reductase activity could also be increased 2-fold after clofibrate was added to the lymphocyte cultures, and was decreased by adding metyrapone so that it was concluded that azoreduction may be partly due to the CYP 4A-family.
Mühlenfeld
K
K
Institute of Pharmacy, Humboldt University, Berlin, Germany.
Langner
A
A
D000818:Animals; D002478:Cells, Cultured; D002994:Clofibrate / Q000494:pharmacology; D003208:Concanavalin A / Q000494:pharmacology; D019363:Cytochrome P-450 CYP1A1 / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000378:metabolism*; D042926:Cytochrome P-450 CYP4A; D065607:Cytochrome P-450 Enzyme Inhibitors; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis / Q000378:metabolism*; D004790:Enzyme Induction / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D000960:Hypolipidemic Agents / Q000494:pharmacology; D007700:Kinetics; D008214:Lymphocytes / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D008797:Metyrapone / Q000494:pharmacology; D006899:Mixed Function Oxygenases / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*
D065607:Cytochrome P-450 Enzyme Inhibitors; D004791:Enzyme Inhibitors; D000960:Hypolipidemic Agents; D003208:Concanavalin A; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases; D019363:Cytochrome P-450 CYP1A1; D042926:Cytochrome P-450 CYP4A; D002994:Clofibrate; D008797:Metyrapone