title: Assessment of a rapid clearance blood pool MR contrast medium (P792) for assays of microvascular characteristics in experimental breast tumors with correlations to histopathology.
abstract: The diagnostic potential of a new rapid clearance blood pool contrast medium (P792; MW = 6.47 kDa) for the MR assessment of microvessel characteristics was assessed in 42 chemically-induced breast tumors, with comparisons to albumin-(Gd-DTPA). Microvessel characteristics, including the transendothelial permeability (K(PS)) and the fractional blood volume (fPV), were estimated by using dynamic MR data fit to a bidirectional two-compartment model. The MR-derived estimates for K(PS) and fPV using each contrast agent were compared, and assays using each contrast agent were correlated to the histologic tumor grade (SBR score) and the microvascular density (MVD) counts. Using P792-enhanced data, neither K(PS) nor fPV showed a statistically significant correlation with the tumor grade or the MVD (P >.05). Conversely, using albumin-(GdDTPA)(30), K(PS) values correlated significantly with the histologic tumor grade (r =.55; P <.0005) and the MVD (r =.34, P <.05), whereas no correlation was established for fPV. In conclusion, based on P792 data no correlation between tumor microvascular characteristics and histologic markers (SBR score or MVD) was found in this breast tumor model. Our analysis suggests that contrast media of relatively large (on the order of 90 kDa) molecular size, such as albumin-(GdDTPA)(30), are more accurate for the characterization of tumor microvessels.
authors.affiliation: Center for Pharmaceutical and Molecular Imaging, Department of Radiology, University of California-San Francisco, 94143-0628, USA.
authors.forename: K
authors.initials: K
authors.lastname: Turetschek
authors.forename: E
authors.initials: E
authors.lastname: Floyd
authors.forename: D M
authors.initials: DM
authors.lastname: Shames
authors.forename: T P
authors.initials: TP
authors.lastname: Roberts
authors.forename: A
authors.initials: A
authors.lastname: Preda
authors.forename: V
authors.initials: V
authors.lastname: Novikov
authors.forename: C
authors.initials: C
authors.lastname: Corot
authors.forename: W O
authors.initials: WO
authors.lastname: Carter
authors.forename: R C
authors.initials: RC
authors.lastname: Brasch
mesh_terms: D000818:Animals; D003287:Contrast Media; D005260:Female; D019786:Gadolinium DTPA; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008325:Mammary Neoplasms, Experimental / Q000098:blood supply* / Q000473:pathology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D003287:Contrast Media; D019786:Gadolinium DTPA