Ebselen protection against hydrogen peroxide-induced cytotoxicity and DNA damage in HL-60 cells.
OBJECTIVE
To study the protective effect of ebselen (Ebs) on hydrogen peroxide (H2O2)-induced cytotoxicity and DNA damage in human leukemia cell line HL-60.


METHODS
The inhibitory effect of H2O2 on cell growth was determined using the tetrazolium dye colorimetric test, and the lipid peroxidation was estimated by malondialdehyde (MDA) formation. DNA damage was detected using single cell gel electrophoresis, and intracellular reactive oxygen species (ROS) formation was measured using a fluorescent probe, 2',7'-dichlorofluorescin diacetate (DCFH-DA).


RESULTS
H2O2 (100 mumol.L-1) suppressed the growth of HL-60 cells and the addition of Ebs (1-20 mumol.L-1) reduced the suppression in a concentration-dependent manner. Furthermore, Ebs also displayed a concentration-dependent reduction of MDA formation in H2O2-treated cells, at the concentration of 20 mumol.L-1 the inhibitory rate was 56.4%. Ebs was able to reduce the ROS formation and DNA damage caused by H2O2 in a concentration-dependent manner.


CONCLUSIONS
Ebs has a strong protective ability against the cytotoxicity and DNA damage caused by reactive oxygen species (ROS).
Li
J
J
College of Basic Medical Sciences, West China University of Medical Sciences. liji58@hotmail.com
Chen
J J
JJ
Zhang
F
F
Zhang
C
C
D000975:Antioxidants / Q000494:pharmacology*; D001393:Azoles / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D004249:DNA Damage / Q000187:drug effects*; D018922:HL-60 Cells; D006801:Humans; D006861:Hydrogen Peroxide / Q000037:antagonists & inhibitors; D054833:Isoindoles; D008315:Malondialdehyde / Q000378:metabolism; D016566:Organoselenium Compounds / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism*
D000975:Antioxidants; D001393:Azoles; D054833:Isoindoles; D016566:Organoselenium Compounds; D017382:Reactive Oxygen Species; C042986:ebselen; D008315:Malondialdehyde; D006861:Hydrogen Peroxide