title: Micromorphological investigation of noncarious cervical lesions treated with demineralizing agents.
abstract: OBJECTIVE
  The purpose of this study was to examine the micromorphology of noncarious cervical lesions (NCCL) and the change occurring after acid conditioning.
  
  
  METHODS
  Nine wedge-shaped lesions, 9 saucer-shaped lesions, and 5 third molars with prepared cervical cavities were used. All NCCLs exhibited a hard, smooth surface with no discoloration. The teeth were sectioned and divided into three groups, each containing the three lesion types. Teeth in Group I were not etched, and teeth in Groups II and III were etched with 35% phosphoric acid and 20% polyacrylic acid/3% aluminum chloride, respectively. Field emission SEM and atomic force microscopy were used to examine the lesion surfaces.
  
  
  RESULTS
  The surfaces of the prepared cervical (control) lesions were covered with a smear layer. Treatment with phosphoric acid and polyacrylic acid resulted in removal of the smear layer, although some residual smear layer was found on the surface treated with polyacrylic acid. The images of the untreated surfaces of NCCL showed highly mineralized dentin with complete obliteration of the dentinal tubules. After treatment with phosphoric and polyacrylic acids, the images revealed demineralization of the surface, which was more pronounced on those treated with phosphoric acid.
  
  
  CONCLUSIONS
  The mineral deposits on the surface of NCCL decreased the effect of acid conditioning. Although there was no apparent difference between the two forms of NCCL used in this study, further work is needed to better understand these lesions.
authors.affiliation: School of Dental Science, University of Melbourne, 711 Elizabeth Street, Melbourne, Victoria 3000, Australia. r.sakoolnamarka@pgrad.unimelb.edu.au
authors.forename: R
authors.initials: R
authors.lastname: Sakoolnamarka
authors.forename: M F
authors.initials: MF
authors.lastname: Burrow
authors.forename: S
authors.initials: S
authors.lastname: Prawer
authors.forename: M J
authors.initials: MJ
authors.lastname: Tyas
mesh_terms: D000134:Acid Etching, Dental; D000180:Acrylic Resins / Q000494:pharmacology; D000077410:Aluminum Chloride; D017607:Aluminum Compounds / Q000494:pharmacology; D001252:Astringents / Q000494:pharmacology; D001641:Bicuspid; D002712:Chlorides / Q000494:pharmacology; D003737:Dental Cavity Preparation; D003804:Dentin / Q000187:drug effects / Q000648:ultrastructure*; D006801:Humans; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D008903:Minerals; D008964:Molar, Third; D010756:Phosphoric Acids / Q000494:pharmacology; D016369:Smear Layer; D019237:Tooth Cervix / Q000187:drug effects / Q000648:ultrastructure*; D014076:Tooth Diseases / Q000473:pathology* / Q000503:physiopathology
chemical_list: D000180:Acrylic Resins; D017607:Aluminum Compounds; D001252:Astringents; D002712:Chlorides; D008903:Minerals; D010756:Phosphoric Acids; D000077410:Aluminum Chloride; C006903:carbopol 940