[Large-capacity expanded cytoline-induced killer cells and its cytotoxic activity].
This study was conducted to establish the large-capacity culture methd of cytokine-induced killer (CIK) cells for clinical therapy and assess its effect on the fuction of cell-mediated immunity following autologous CIK cells reinfusion. Autologus CIK cells were expanded in 1000 ml culture-bag and reinfused back. The MTT method was used to test the cytotoxic activity of CIK cells before and after reinfusion. The results showed that the total amount of autologous CIK cells reinfusion exceeded 1.6 x 10(10) with the use of the culture method of large-capacity. The PBMNC from patients treated by CIK cells showed significant increase in cytotoxic activity, no side effects were observed, and therefore the large-capacity culture method of CIK cells is a simle and safe therapy for treating the minimum residue of diseases.
Shi
Y
Y
Department of Hematology, First Hosital, Beijing University, Beijing 100034.
Yu
J
J
Cen
X
X
Zhu
P
P
Ma
M
M
D000328:Adult; D000368:Aged; D016207:Cytokines / Q000494:pharmacology; D019337:Hematologic Neoplasms / Q000276:immunology / Q000628:therapy*; D006801:Humans; D007111:Immunity, Cellular; D016219:Immunotherapy, Adoptive; D008875:Middle Aged; D016260:Monocytes, Activated Killer / Q000187:drug effects / Q000276:immunology*; D014407:Tumor Cells, Cultured
D016207:Cytokines