[Human lens epithelial cells culture in vitro].
OBJECTIVE
To provide a simple and rapid procedure to culture human len epithelial cells in vitro.


METHODS
Specimens of capsule lentils were isolated from lens by microscissors. They were cut into small segments and then directly implanted into basks(culture bottles). The tissue pieces were cultured in a incubation at 37 degrees C with PR1640 until attached cells reached confluence. The len epithelium were passaged by tropism.


RESULTS
Len epithelial cells usually appeared after 4 days in incubation. The cells growed rapidly in a way of attachments.


CONCLUSIONS
Len epithelial cells were easily cultured with our method. The procedure is simple, rapid and highly successful.
Zheng
J
J
Zhongshan Ophthalmic Center, Sun Yat-sen University of Medical Sciences, Guangzhou 510060, China.
Liu
Y
Y
Peng
Y
Y
D018929:Cell Culture Techniques / Q000379:methods; D004847:Epithelial Cells / Q000166:cytology*; D006801:Humans; D007908:Lens, Crystalline / Q000166:cytology*