The est1 regulation depends on the oxygen concentration in Acetobacter pasteurianus.
The regulation mechanism for expression of the ethanol inducible esterase gene, est1, was investigated in A. pasteurianus. Deletion analysis of the 5' non coding region of est1 showed that the FNR-binding consensus sequence is important in the induction of est1 by ethanol. Cells grown under oxygen starvation produced esterase-1 in not only the presence but also the absence of ethanol. These results suggest that the induction of est1-expression depends on the oxygen concentration, and the gene may be induced by a FNR-like factor activated by a decrease in the intracellular oxygen concentration.
Kashima
Y
Y
Department of Fermentation Science, Faculty of Applied Bio-Science, Tokyo University of Agriculture, Japan.
Nakajima
Y
Y
Kosugi
A
A
Tayama
K
K
Koizumi
Y
Y
Udaka
S
S
Yanagida
F
F
D000426:Alcohol Dehydrogenase / Q000235:genetics; D001483:Base Sequence; D004950:Esterases / Q000235:genetics*; D000431:Ethanol; D015971:Gene Expression Regulation, Enzymologic; D008969:Molecular Sequence Data; D010100:Oxygen / Q000378:metabolism
D000431:Ethanol; D000426:Alcohol Dehydrogenase; D004950:Esterases; D010100:Oxygen