title: Catalog of 320 single nucleotide polymorphisms (SNPs) in 20 quinone oxidoreductase and sulfotransferase genes.
abstract: Single nucleotide polymorphisms (SNPs) in genes encoding drug-metabolizing enzymes, transporters, receptors, and other drug targets have been widely implicated as contributors to differences among individuals as regards the efficacy and toxicity of many medications, as well as the susceptibility to complex diseases. By combining the polymerase chain reaction (PCR) technique with direct sequencing, we screened genomic DNAs from 48 Japanese volunteers for SNPs in genes encoding three quinone oxidoreductases (NQO1, NQO2, and PIG3) and 17 sulfotransferases (SULT1A1, SULT1A2, SULT1A3, SULT1C1, SULT1C2, SULT2A1, SULT2B1, ST1B2, TPST1, TPST2, SULTX3, STE, CST, HNK-1 ST, CHST2, CHST4, and CHST5). In all, we identified 320 SNPs from these 20 loci: 22 within coding elements, 21 in 5' flanking regions, 10 in 5' untranslated regions, 223 in introns, 19 in 3' untranslated regions, and 25 in 3' flanking regions. The ratio of transitions to transversions was approximately 2.3 to 1. Of the 22 coding SNPs, 6 were nonsynonymous substitutions that resulted in amino-acid substitutions. The high-density SNP maps we constructed from this data for each of the quinone oxidoreductases and sulfotransferases examined here should provide useful information for investigations designed to detect association(s) between genetic variations and common diseases or responsiveness to drug therapy.
authors.affiliation: Laboratory for Genotyping, The SNP Research Center, The Institute of Physical and Chemical Research (RIKEN), Tokyo, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Iida
authors.forename: A
authors.initials: A
authors.lastname: Sekine
authors.forename: S
authors.initials: S
authors.lastname: Saito
authors.forename: Y
authors.initials: Y
authors.lastname: Kitamura
authors.forename: T
authors.initials: T
authors.lastname: Kitamoto
authors.forename: S
authors.initials: S
authors.lastname: Osawa
authors.forename: C
authors.initials: C
authors.lastname: Mishima
authors.forename: Y
authors.initials: Y
authors.lastname: Nakamura
mesh_terms: D001483:Base Sequence; D005091:Exons; D014644:Genetic Variation; D006801:Humans; D007438:Introns; D007564:Japan; D008969:Molecular Sequence Data; D004364:Pharmaceutical Preparations / Q000378:metabolism; D020641:Polymorphism, Single Nucleotide; D011808:Quinone Reductases / Q000235:genetics*; D017422:Sequence Analysis, DNA; D015238:Sulfotransferases / Q000235:genetics*; D020506:Untranslated Regions
chemical_list: D004364:Pharmaceutical Preparations; D020506:Untranslated Regions; D011808:Quinone Reductases; D015238:Sulfotransferases