title: Reaction-induced infrared difference spectroscopy for the study of protein reaction mechanisms.
abstract: This paper reviews state-of-the-art reaction-induced infrared difference spectroscopy of proteins. This technique enables detailed characterization of enzyme function on the level of single bonds of proteins, cofactors, or substrates. The following methods to initiate a reaction in the infrared sample are discussed: (i) light-induced difference spectroscopy, (ii) attenuated total reflection with buffer exchange, (iii) the infrared variant of stopped and continuous flow, (iv) temperature and pressure jump, (v) photolytical release of effector substances from caged compounds, (vi) equilibrium electrochemistry, and (vii) photoreduction. Illustrating applications are given including hot topics from the fields of bioenergetics, protein folding, and molecule--protein interaction.
authors.affiliation: Institut für Biophysik, Johann Wolfgang Goethe-Universität, Theodor-Stern-Kai 7, Haus 74, D-60590 Frankfurt am Main, Germany. zscherp@biophysik.uni-frankfurt.de
authors.forename: C
authors.initials: C
authors.lastname: Zscherp
authors.forename: A
authors.initials: A
authors.lastname: Barth
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism*; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods; D010777:Photochemistry / Q000295:instrumentation / Q000379:methods; D013055:Spectrophotometry, Infrared / Q000295:instrumentation / Q000379:methods*; D017550:Spectroscopy, Fourier Transform Infrared / Q000295:instrumentation / Q000379:methods; D013329:Structure-Activity Relationship
chemical_list: D001426:Bacterial Proteins