A helical lid converts a sulfotransferase to a dehydratase.
We report here the crystal structure of retinol dehydratase, an enzyme that catalyzes the synthesis of anhydroretinol. The enzyme is a member of the sulfotransferase superfamily and its crystal structure reveals the insertion of a helical lid into a canonical sulfotransferase fold. Site-directed mutations demonstrate that this inserted lid is necessary for anhydroretinol production but not for sulfonation; thus, insertion of a helical lid can convert a sulfotransferase into a dehydratase.
Pakhomova
S
S
Biochemistry Department, Vanderbilt University School of Medicine, Nashville, Tennessee 37232-0146, USA.
Kobayashi
M
M
Buck
J
J
Newcomer
M E
ME
D000595:Amino Acid Sequence; D019943:Amino Acid Substitution / Q000235:genetics; D000818:Animals; D001547:Benzaldehydes / Q000378:metabolism; D001665:Binding Sites; D018360:Crystallography, X-Ray; D006836:Hydro-Lyases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D017433:Protein Structure, Secondary; D016415:Sequence Alignment; D013329:Structure-Activity Relationship; D013431:Sulfates / Q000378:metabolism; D015238:Sulfotransferases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D014801:Vitamin A / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology
D001547:Benzaldehydes; D013431:Sulfates; D014801:Vitamin A; C031469:anhydrovitamin A; C100058:vanillin; D015238:Sulfotransferases; D006836:Hydro-Lyases; C100603:retinol dehydratase