title: RAD51-independent break-induced replication to repair a broken chromosome depends on a distant enhancer site.
abstract: Without the RAD51 strand exchange protein, Saccharomyces cerevisiae cannot repair a double-strand break (DSB) by gene conversion. However, cells can repair DSBs by recombination-dependent, break-induced replication (BIR). RAD51-independent BIR is initiated more than 13 kb from the DSB. Repair depends on a 200-bp sequence adjacent to ARS310, located approximately 34 kb centromere-proximal to the DSB, but does not depend on the origin activity of ARS310. We conclude that the ability of a recombination-induced replication fork to copy > 130 kb to the end of the chromosome depends on a special site that enhances assembly of a processive repair replication fork.
authors.affiliation: Rosenstiel Center and Department of Biology, Brandeis University, Waltham, Massachusetts 02254-9110, USA.
authors.forename: A
authors.initials: A
authors.lastname: Malkova
authors.forename: L
authors.initials: L
authors.lastname: Signon
authors.forename: C B
authors.initials: CB
authors.lastname: Schaefer
authors.forename: M L
authors.initials: ML
authors.lastname: Naylor
authors.forename: J F
authors.initials: JF
authors.lastname: Theis
authors.forename: C S
authors.initials: CS
authors.lastname: Newlon
authors.forename: J E
authors.initials: JE
authors.lastname: Haber
mesh_terms: D001483:Base Sequence; D015825:Chromosomes, Fungal; D004260:DNA Repair / Q000502:physiology*; D004261:DNA Replication; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D004742:Enhancer Elements, Genetic; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D051135:Rad51 Recombinase; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins
chemical_list: D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D029701:Saccharomyces cerevisiae Proteins; C495151:RAD51 protein, S cerevisiae; D051135:Rad51 Recombinase