Effect of human follicular fluid on sperm survival.
OBJECTIVE
To study the effect of follicular fluid (FF) on sperm survival.


METHODS
Swim-up sperm suspensions obtained from 20 men with normal semen analysis were incubated with Ham's F-10 only and Ham's F-10 supplemented with 20% FF or 20% serum. Sperm motility was recorded every 12 hours for 72 hours.


RESULTS
Sperm motility was maintained in all media for 48 hours. However, significantly more sperm samples remained motile at 72 hours in medium supplemented with FF and serum as compared to Ham's F-10 only.


CONCLUSIONS
FF has a positive effect on conserving sperm motility as a function of time.
Abu-Musa
A
A
Department of Obstetrics and Gynecology, American University of Beirut, Lebanon.
Khalil
A
A
Hannoun
A
A
Sakhel
K
K
Takahashi
K
K
D002470:Cell Survival; D005260:Female; D015571:Follicular Fluid; D006801:Humans; D008297:Male; D013081:Sperm Motility; D013094:Spermatozoa / Q000502:physiology*