[Analysis of the major components in cannabis by capillary gas chromatography].
A capillary GC method for the simultaneous determination of cannabidiol (CBD), tetrahydrocannabinol (THC), and cannabinol (CBN) in cannabis is described. The major components in cannabis were extracted with chloroform. After being concentrated by evaporation, the residue was dissolved in methanol. The resulting solution was used for analysis. The conditions for determination were: FID detector, HP-5 capillary column, 10 m x 0.53 mm x 2.65 microns film, column temperature 220 degrees C. The recoveries of the three major components were 97.3%-104.0%(CBD), 97.3%-106.6%(THC) and 95.3%-102.4% (CBN). The coefficients of variation are less than 3%. The limit of detection for the three components was 0.2 mg/L, and their linearity was in the range of 20-120 mg/L. Identification of each component was achieved by comparison of retention times with the standards. This method has the advantages of simplicity, rapidity, sensitivity and accuracy.
Peng
X
X
Shanghai Institute for Drug Control, Shanghai, 200233.
D002185:Cannabidiol / Q000032:analysis*; D002187:Cannabinol / Q000032:analysis; D002188:Cannabis / Q000737:chemistry*; D002849:Chromatography, Gas / Q000379:methods*; D013759:Dronabinol / Q000032:analysis*
D002185:Cannabidiol; D013759:Dronabinol; D002187:Cannabinol