title: Protein-binding polynomials.
abstract: Using a new method recently published for analyzing the binding isotherms of biopolymers (Poland, 2000a), we calculate the complete binding polynomials for lysozyme, insulin, and serum albumin from published titration data. These three proteins have, respectively, 22, 32, and 184 dissociable protons and hence are represented by series in powers of the hydrogen ion concentration with the highest powers in the series being the numbers just indicated. Given the complete binding polynomial, the distribution function giving the concentration of all states of proton binding can then be calculated at any pH.
authors.affiliation: Department of Chemistry, Johns Hopkins University, Baltimore, Maryland 21218, USA. poland@jhunix.hcf.jhu.edu
authors.forename: D
authors.initials: D
authors.lastname: Poland
mesh_terms: D006863:Hydrogen-Ion Concentration; D007328:Insulin / Q000737:chemistry / Q000378:metabolism; D008956:Models, Chemical; D009113:Muramidase / Q000737:chemistry / Q000378:metabolism; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism*; D011522:Protons; D012709:Serum Albumin / Q000737:chemistry / Q000378:metabolism; D017186:Titrimetry
chemical_list: D007328:Insulin; D011506:Proteins; D011522:Protons; D012709:Serum Albumin; D009113:Muramidase