Molecular cloning and analysis of the 5'-flanking region of the human MYPT1 gene.
We have cloned and sequenced a 5 kb genomic fragment in the 5'-flanking region of the human myosin phosphatase target subunit 1. The transcription initiation site (+1) was 268 bp upstream from the translation start site. In this promoter there are no canonical TATA or CAAT box elements but there is a high GC-rich sequence. Basal promoter activity was due to the GC-rich region that contained one Sp1 transcription factor binding site, thus demonstrating that the MYPT1 gene is a housekeeping gene. Luciferase reporter assays showed the presence of two regions for positive elements and one for a negative element.
Machida
H
H
First Department of Internal Medicine, University School of Medicine, Tsu, Mie, Japan.
Ito
M
M
Okamoto
R
R
Shiraki
K
K
Isaka
N
N
Hartshorne
D J
DJ
Nakano
T
T
D001483:Base Sequence; D001665:Binding Sites; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D008156:Luciferases / Q000235:genetics; D008969:Molecular Sequence Data; D043263:Myosin-Light-Chain Phosphatase; D010749:Phosphoprotein Phosphatases / Q000737:chemistry / Q000235:genetics*; D011401:Promoter Regions, Genetic; D021122:Protein Subunits; D016329:Sp1 Transcription Factor / Q000378:metabolism; D014158:Transcription, Genetic
D017931:DNA Primers; D021122:Protein Subunits; D016329:Sp1 Transcription Factor; D004247:DNA; D008156:Luciferases; D010749:Phosphoprotein Phosphatases; D043263:Myosin-Light-Chain Phosphatase