title: Calcium store depletion induced by mitochondrial uncoupling in prostatic cells.
abstract: The effects of mitochondrial uncoupling on the calcium homeostasis of prostatic cells were investigated using the prostatic cancer cell line LNCaP and indo-1 spectrofluorimetry. Carbonyl cyanide m-chloro-phenylhydrazone (CCCP) was used as uncoupler. Resting LNCaP cells responded to CCCP by a biphasic increase in [Ca2+]i. The first phase of increase which corresponded to the release of a mitochondrial CCCP-sensitive Ca2+ store was followed by a second increase phase consisting of Ca2+ influx through the plasma membrane. The relationship between the CCCP- and the InsP3-sensitive stores was investigated using thapsigargin (TG). The release part of the Ca2+ response to TG was reduced in a time-dependent manner by previous exposure of the cells to CCCP, suggesting that CCCP also acts on non-mitochondrial stores. Our results show that CCCP releases Ca2+ from both mitochondrial and non-mitochondrial stores in prostatic cells. The possible mechanisms of these effects are discussed.
authors.affiliation: Laboratoire de Neurophysiologie, CNRS UMR 5543, Université Victor Segalen-Bordeaux II, France.
authors.forename: S
authors.initials: S
authors.lastname: Vaur
authors.forename: P
authors.initials: P
authors.lastname: Sartor
authors.forename: L
authors.initials: L
authors.lastname: Dufy-Barbe
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000968:Antimycin A / Q000031:analogs & derivatives* / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology; D002462:Cell Membrane / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005456:Fluorescent Dyes / Q000494:pharmacology; D006801:Humans; D007211:Indoles / Q000494:pharmacology; D008297:Male; D008928:Mitochondria / Q000378:metabolism*; D009840:Oligomycins / Q000494:pharmacology; D011471:Prostatic Neoplasms / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D019284:Thapsigargin / Q000494:pharmacology; D013997:Time Factors; D014407:Tumor Cells, Cultured; D014475:Uncoupling Agents / Q000494:pharmacology*
chemical_list: D004791:Enzyme Inhibitors; D005456:Fluorescent Dyes; D007211:Indoles; D009840:Oligomycins; D014475:Uncoupling Agents; C032456:antimycin; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D000968:Antimycin A; D019284:Thapsigargin; D000255:Adenosine Triphosphate; C048960:indo-1; D002118:Calcium