Structure of the H subunit of the photosynthetic reaction center from the thermophilic purple sulfur bacterium, Thermochromatium tepidum Implications for the specific binding of the lipid molecule to the membrane protein complex.
The photosynthetic reaction center (RC) is a transmembrane protein complex that catalyzes light-driven electron transport across the photosynthetic membrane. The complete amino-acid sequence of the H subunit of the RC from a thermophilic purple sulfur bacterium, Thermochromatium tepidum, has been determined for the first time among purple sulfur bacteria. The H subunit consists of 259 amino acids and has a molecular mass of 28 187. The deduced amino-acid sequences of this H subunit showed a significant (40%) degree of identity with those from mesophilic purple nonsulfur bacteria. The determined primary structure of the H subunit was compared with the structures of mesophilic B. viridis and R. sphaeroides based on the three-dimensional structure of the H subunit from T. tepidum, which has been recently determined by X-ray crystallography. One lipid molecule was found in the crystal structure of the T. tepidum RC, and the head group of the lipid appears to be stabilized by the electrostatic interactions with the conserved basic residues in the H subunit. The above comparison has suggested the existence of a lipid-binding site on the molecular surface at which a lipid molecule can interact with the RC in a specific manner.
Fathir
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Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University, Aoba-ku, Sendai, Japan.
Mori
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T
Nogi
T
T
Kobayashi
M
M
Miki
K
K
Nozawa
T
T
D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites; D002841:Chromatiaceae / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D018360:Crystallography, X-Ray; D017931:DNA Primers / Q000235:genetics; D008563:Membrane Lipids / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D021122:Protein Subunits; D012242:Rhodobacter sphaeroides / Q000737:chemistry / Q000235:genetics; D012241:Rhodopseudomonas / Q000737:chemistry / Q000235:genetics; D017386:Sequence Homology, Amino Acid
D017931:DNA Primers; D008563:Membrane Lipids; D008565:Membrane Proteins; D045322:Photosynthetic Reaction Center Complex Proteins; D021122:Protein Subunits