title: Rotavirus G and P genotypes in rural Ghana.
abstract: An epidemiological study of rotavirus infection was conducted on specimens collected from patients with gastroenteritis and domiciled in the rural Upper Eastern Region of Ghana during 1998. Fifty isolates, randomly selected from 165 human group A rotavirus-positive samples, were G and P characterized by a reverse transcription (RT)-PCR assay using a seminested multiplex method. Rotaviruses of the G3 genotype were found to be the predominant strain (78%), followed by G2 (14%) and G1 (2%). Mixed infections, as shown by combinations of G3 and G2 (4%) and G3 and G1 (2%), were also observed. P typing showed P[4] (72.34%) to be the prevalent strain, followed by P[6] (21.3%), P[8] (2.13%), and a combination of P[4] and P[6] (4.3%).
authors.affiliation: Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana, Africa. RAsmah@noguchi.mimcon.net
authors.forename: R H
authors.initials: RH
authors.lastname: Asmah
authors.forename: J
authors.initials: J
authors.lastname: Green
authors.forename: G E
authors.initials: GE
authors.lastname: Armah
authors.forename: C I
authors.initials: CI
authors.lastname: Gallimore
authors.forename: J J
authors.initials: JJ
authors.lastname: Gray
authors.forename: M
authors.initials: M
authors.lastname: Iturriza-Gómara
authors.forename: F
authors.initials: F
authors.lastname: Anto
authors.forename: A
authors.initials: A
authors.lastname: Oduro
authors.forename: F N
authors.initials: FN
authors.lastname: Binka
authors.forename: D W
authors.initials: DW
authors.lastname: Brown
authors.forename: F
authors.initials: F
authors.lastname: Cutts
mesh_terms: D002675:Child, Preschool; D005759:Gastroenteritis / Q000453:epidemiology / Q000821:virology; D005838:Genotype; D005869:Ghana / Q000453:epidemiology; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012401:Rotavirus / Q000145:classification* / Q000235:genetics*; D012400:Rotavirus Infections / Q000453:epidemiology* / Q000821:virology; D012424:Rural Population