title: T2 mapping of rat patellar cartilage.
abstract: OBJECTIVE
  To investigate the usefulness of magnetic resonance (MR) T2 mapping in characterizing the evolution of cartilage matrix content and thickness during the maturation and aging process.
  
  
  METHODS
  Patellae from four groups of rats aged 4 weeks, 8 weeks, 4 months, and more than 6 months ("old rats") were studied ex vivo with an 8.5-T microimager. T2 values were calculated on transverse rat patellar sections and displayed with a color scale (the T2 map) on a pixel-by-pixel basis. Biochemical and histologic studies were performed to evaluate the influence of proteoglycans and collagen contents on T2 values of the patellar cartilage.
  
  
  RESULTS
  On the T2 map, the maturation process until 10 weeks was characterized by a decrease in T2 values and in cartilage thickness. The biochemical data revealed a global decrease in proteoglycans and a progressive global increase in collagen content, whereas the histologic study revealed subtle zonal variation in matrix constituents with depth. As aging progressed, the T2 values were low, without important variations, whereas the global cartilage thickness decreased. The cartilage matrix became globally more fibrotic, especially in the deepest zone. Biochemical analysis revealed that collagen content was more determinant of MR signal intensity than was proteoglycans content during maturation and aging.
  
  
  CONCLUSIONS
  T2 mapping allows characterization of variations in cartilage matrix constituents and thickness.
authors.affiliation: Department of Pharmacology, UMR 7561, CNRS-Université Henri Poincaré, Nancy I, France.
authors.forename: A
authors.initials: A
authors.lastname: Watrin
authors.forename: J P
authors.initials: JP
authors.lastname: Ruaud
authors.forename: P T
authors.initials: PT
authors.lastname: Olivier
authors.forename: N C
authors.initials: NC
authors.lastname: Guingamp
authors.forename: P D
authors.initials: PD
authors.lastname: Gonord
authors.forename: P A
authors.initials: PA
authors.lastname: Netter
authors.forename: A G
authors.initials: AG
authors.lastname: Blum
authors.forename: G M
authors.initials: GM
authors.lastname: Guillot
authors.forename: P M
authors.initials: PM
authors.lastname: Gillet
authors.forename: D H
authors.initials: DH
authors.lastname: Loeuille
mesh_terms: D000375:Aging; D000818:Animals; D002358:Cartilage, Articular / Q000737:chemistry* / Q000166:cytology / Q000254:growth & development*; D011508:Chondroitin Sulfate Proteoglycans / Q000032:analysis; D003094:Collagen / Q000032:analysis; D006909:Hydroxyproline / Q000032:analysis; D008279:Magnetic Resonance Imaging; D008297:Male; D010329:Patella; D051381:Rats; D017208:Rats, Wistar
chemical_list: D011508:Chondroitin Sulfate Proteoglycans; D003094:Collagen; D006909:Hydroxyproline