Limiting light-induced lipid peroxidation and vitamin loss in infant parenteral nutrition by adding multivitamin preparations to Intralipid.
Parenteral lipids are susceptible to light-induced peroxidation, particularly under phototherapy. Ascorbic acid is protective. The aim of this study was to investigate whether dark delivery tubing and/or coadministration of multivitamin preparations could prevent peroxidation of Intralipid without undue vitamin loss. In experiments carried out on the benchtop, lipid peroxidation occurred in ambient light and was more extensive under phototherapy. Dark tubing decreased peroxide formation, but only by about 65%. In simulated clinical conditions in which solutions were pumped through standard clear or dark minibore plastic tubing. Intralipid accumulated lipid peroxides as measured by the FOX assay (280 microM) or as triglyceride hydroperoxides (52 microM). Multivitamin preparations (MVIP or Soluvit/Vitlipid) inhibited peroxide formation almost completely, and were fully protective when used with dark tubing. There was loss of riboflavin (65% from Soluvit and 35% from MVIP) in clear tubing but this was decreased to 18% and 11%, respectively, in dark tubing. Ascorbate loss was 20% (MVIP) and 50% (Soluvit) and only slightly less in dark tubing. Ascorbate loss was also seen in the absence of Intralipid and is due to riboflavin-induced photo-oxidation.



Multivitamin preparations protect Intralipid against light-induced formation of lipid hydroperoxides, and administering multivitamins with Intralipid via dark delivery tubing provides a practical way of preventing peroxidation of the lipid while limiting vitamin loss. This procedure should be considered for routine use as well as with phototherapy.
Silvers
K M
KM
Free Radical Research Group, Department of Pathology, Christchurch School of Medicine, New Zealand.
Sluis
K B
KB
Darlow
B A
BA
McGill
F
F
Stocker
R
R
Winterbourn
C C
CC
D001205:Ascorbic Acid; D005217:Fat Emulsions, Intravenous / Q000378:metabolism*; D005526:Food, Formulated; D006801:Humans; D007231:Infant, Newborn; D007234:Infant, Premature; D008027:Light / Q000009:adverse effects*; D015227:Lipid Peroxidation / Q000528:radiation effects; D010288:Parenteral Nutrition / Q000295:instrumentation; D010789:Phototherapy; D012256:Riboflavin; D014810:Vitamin E; D014815:Vitamins / Q000378:metabolism*
D005217:Fat Emulsions, Intravenous; D014815:Vitamins; D014810:Vitamin E; D001205:Ascorbic Acid; D012256:Riboflavin