Sensitive 1H-31P correlations with 5' methylene protons of DNA via homonuclear double-quantum coherence.
A novel pulse sequence is presented for the correlation of 5' and 5'' protons in DNA with phosphorus. Double-quantum coherence between the methylene protons is used to generate 1H5'-31P and 1H5''-31P cross peaks in an HMQC-type experiment. The resolution for these cross peaks is significantly improved over that of conventional HSQC experiments, as cross peaks between 1H4' and 31P are largely suppressed and a 3D version of the experiment can be performed with little penalty in sensitivity. In addition, sensitivity is favoured by slower relaxation of the double-quantum coherence and a more favourable multiplet fine structure in the acquisition dimension.
Kaikkonen
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A
Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden.
Otting
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G
D004247:DNA / Q000737:chemistry*; D006859:Hydrogen / Q000737:chemistry; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D010759:Phosphorus Isotopes; D011522:Protons
D010759:Phosphorus Isotopes; D011522:Protons; D006859:Hydrogen; D004247:DNA