[Construction of chicken hypothalamus cDNA library and primary analysis of ESTs sequences from partial clones].
Using hypothalamus as material and lambda gt10 as vector, chicken hypothalamus cDNA library has been constructed. The results showed that the titration of the cDNA library we constructed was 3.8 x 10(6), and the fragment length of inserted DNA ranged mainly from 1.0-3.0 kb. The ESTs was sequenced and primarily analyzed for ten clones randomly selected from above library. After analysis with BLASTn and FASTA3.1, it was found that four ESTs developed matched homologous sequence in chicken, four also had similar sequences in human and other species, have other two sequences were function-unknown gene. The sequences of ten ESTs we studied have been loaded into GenBank.
Zhao
Z H
ZH
National Lab of AgroBiotechnology, China Agriculture University, Beijing 100094, China.
Li
N
N
Liu
Z L
ZL
Zhang
Y J
YJ
Wu
C X
CX
D000818:Animals; D002645:Chickens; D018076:DNA, Complementary / Q000737:chemistry*; D020224:Expressed Sequence Tags; D015723:Gene Library; D007031:Hypothalamus / Q000378:metabolism*
D018076:DNA, Complementary