Applicability of micellar electrokinetic chromatography to the analysis of estrogens in water.
Estrone, beta-estradiol and ethynylestradiol spiked in water were extracted using solid-phase extraction (SPE) and then directly determined by micellar electrokinetic chromatography (MEKC) with online concentration (sweeping). A 350 mL original sample volume (10 nM each) was concentrated to 1 mL using SPE, and ca. 240 nL of this solution was injected onto an MEKC capillary column. After sweeping, the estrogen related compounds were detected using a commercial absorbance detection system with an LOD of 0.16-0.30 nM in the original sample.
Harino
H
H
Osaka City Institute of Public Health and Environmental Sciences, Tennoji, Japan.
Tsunoi
S
S
Sato
T
T
Tanaka
M
M
D002138:Calibration; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D004967:Estrogens / Q000032:analysis*; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D014874:Water Pollutants, Chemical / Q000032:analysis*
D004967:Estrogens; D014874:Water Pollutants, Chemical