Determination of nucleic acids with crystal violet by a resonance light-scattering technique.
For the first time, Crystal Violet (CV) was used to determine nucleic acid concentrations using the resonance light-scattering (RLS) technique. Based on the enhancement of the RLS of CV by nucleic acids, a new quantitative determination method for nucleic acids in aqueous solutions has been developed. At pH 5.03 and ionic strength 0.005 mol kg-1, the interaction of CV with nucleic acids results in three characteristic RLS peaks at 344.0, 483.0 and 666.0 nm. With 4.0 x 10(-5) mol l-1 of CV, linear relationships were found between the enhanced intensity of RLS at 666.0 nm and the concentration of nucleic acids in the range 0-2.5 micrograms ml-1 for herring sperm DNA, 0-4.0 micrograms ml-1 for calf thymus DNA and 0-4.5 micrograms ml-1 for yeast RNA. The limits of determination were 13.8 ng ml-1 for herring sperm DNA, 36.8 ng ml-1 for calf thymus DNA and 69.0 ng ml-1 for yeast RNA. The assay is convenient, rapid, inexpensive and simple.
Zhang
W
W
Department of Chemistry, Lanzhou University, Lanzhou 730000, China. wjzhang0075@sina.com.cn
Xu
H
H
Wu
S
S
Chen
X
X
Hu
Z
Z
D000818:Animals; D002417:Cattle; D004247:DNA / Q000032:analysis; D005399:Fishes / Q000235:genetics; D005840:Gentian Violet; D008027:Light; D009696:Nucleic Acids / Q000032:analysis*; D012331:RNA, Fungal / Q000032:analysis; D012542:Scattering, Radiation; D013050:Spectrometry, Fluorescence / Q000295:instrumentation
D009696:Nucleic Acids; D012331:RNA, Fungal; D004247:DNA; D005840:Gentian Violet