[Role of the antioxidant system in response of Escherichia coli bacteria to cold stress].
The response of aerobically grown Escherichia coli cells to the cold shock induced by the rapid lowering of growth temperature from 37 to 20 degrees C was found to be basically the same as the oxidative stress response. The enhanced sensitivity of cells deficient in two superoxide dismutases, Mn-SOD and Fe-SOD, and the increased expression of the Mn-SOD gene, sodA, in response to cold stress were interpreted as both oxidative and cold stresses are due to a rise in the intracellular level of superoxide anion. The long-term cultivation of E. coli at 20 degrees C was also accompanied by the typical oxidative stress response reactions--an enhanced expression of the Mn-SOD and catalase HPI genes and a decrease in the intracellular level of reduced glutathione (GSH) and in the GSH/GSSG ratio.
Smirnova
G V
GV
Institute of Ecology and Genetics of Microorganisms, Ural Division, Russian Academy of Sciences, ul. Lenina 11, Perm, 614600 Russia.
Zakirova
O N
ON
Oktiabr'skiĭ
O N
ON
D000975:Antioxidants / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D003080:Cold Temperature; D004926:Escherichia coli / Q000201:enzymology / Q000378:metabolism* / Q000502:physiology; D005978:Glutathione / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism
D000975:Antioxidants; D002374:Catalase; D013482:Superoxide Dismutase; D005978:Glutathione