Crystallization and preliminary X-ray analysis of cardiotoxic phospholipase A2 from Ophiophagus hannah (king cobra).
An acidic phospholipase A2 exhibiting cardiotoxicity, myotoxicity and anti-platelet activity was isolated from Ophiophagus hannah (king cobra) from Guangxi, China. It contains an unusual 'pancreatic loop'. The enzyme was purified to homogeneity and crystallized using polyethylene glycol and ethylene glycol as precipitants. The crystal belongs to space group C2, with unit-cell parameters a = 117.92, b = 62.94, c = 57.16 A, beta = 100.93 degrees. Diffraction data were collected to 2.6 A.
Wang
Z
Z
Guangxi Medical University, Nanning 530021, China.
Zhuang
M
M
Shu
Y
Y
Zhang
H
H
Song
S
S
Lin
Z
Z
D000818:Animals; D003460:Crystallization; D018360:Crystallography, X-Ray; D004546:Elapid Venoms / Q000201:enzymology*; D017815:Elapidae; D006321:Heart / Q000187:drug effects; D010741:Phospholipases A / Q000737:chemistry* / Q000633:toxicity; D054467:Phospholipases A2; D011487:Protein Conformation
D004546:Elapid Venoms; D010741:Phospholipases A; D054467:Phospholipases A2