Regulation of human oviductin mRNA expression in vivo.
OBJECTIVE
To examine changes in oviductin mRNA expression in oviductal mucosal tissue from fertile women throughout an ovulatory cycle.


METHODS
Semiquantitative reverse-transcriptase-polymerase chain reaction (RT-PCR) analysis of oviductin mRNA.


METHODS
University-based obstetrics and gynecology department.


METHODS
Twenty women undergoing laparoscopy for tubal sterilization or hysterectomy for uterine fibroids.


METHODS
The mucosal layer was isolated from the oviduct tissue, and semiquantitative RT-PCR was performed.


METHODS
The relationship between serum estradiol, luteinizing hormone, and progesterone concentrations and the expression of oviductin mRNA.


RESULTS
There was a significant positive correlation between serum estradiol and luteinizing hormone concentrations and oviductin mRNA expression. There was a significant inverse correlation between serum progesterone concentrations and oviductin mRNA expression.


CONCLUSIONS
Little is known about the regulation of human oviductin. This study was the first to examine the relationship between oviductin mRNA expression and serum estradiol and luteinizing hormone and progesterone concentrations in fertile women. Estradiol and luteinizing hormone both have a stimulatory effect on oviductin mRNA in humans, however, it is difficult to determine whether the effects are independent of one another, as the luteinizing hormone surge is dependent on the estradiol increase. Progesterone shows a clear inhibitory effect on oviductin mRNA.
Briton-Jones
C
C
Department of Obstetrics and Gynaecology, Prince of Wales Hospital, Shatin, New Territories, Hong Kong, China. chrisbj@cuhk.edu.hk
Lok
I H
IH
Yuen
P M
PM
Chiu
T T
TT
Cheung
L P
LP
Haines
C
C
D000328:Adult; D004958:Estradiol / Q000097:blood; D005187:Fallopian Tubes / Q000378:metabolism* / Q000502:physiology; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D007986:Luteinizing Hormone / Q000097:blood; D008597:Menstrual Cycle / Q000502:physiology*; D011374:Progesterone / Q000097:blood; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012697:Serine Endopeptidases / Q000096:biosynthesis / Q000235:genetics*; D018709:Statistics, Nonparametric
D012333:RNA, Messenger; D011374:Progesterone; D004958:Estradiol; D007986:Luteinizing Hormone; D012697:Serine Endopeptidases; C074599:oviductin