Disrupting Il13 impairs production of IL-4 specified by the linked allele.
Interleukin 13-deficient (IL-13-/-) mice express a defect in priming for IL-4 production that is not corrected by adding IL-13 to the priming culture. This is partly accounted for by the consumption of IL-4 without endogenous replacement during culture of IL-13-/- CD4+ T cells. We examined cells from mice in which disrupted Il13 was linked to wild-type Il4 on one chromosome and wild-type Il13 was linked to a "knocked-in" green fluorescent protein (Gfp) gene in the Il4 locus. Our results show that the deficit in IL-4 production was due, at least in part, to a cis effect, in which disrupted Il13 diminished IL-4 production from the linked Il4 gene.
Guo
L
L
Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
Hu-Li
J
J
Zhu
J
J
Pannetier
C
C
Watson
C
C
McKenzie
G J
GJ
McKenzie
A N
AN
Paul
W E
WE
D000483:Alleles; D000818:Animals; D015870:Gene Expression; D005786:Gene Expression Regulation; D008040:Genetic Linkage; D018793:Interleukin-13 / Q000096:biosynthesis / Q000235:genetics*; D015847:Interleukin-4 / Q000096:biosynthesis / Q000235:genetics*; D051379:Mice; D008817:Mice, Mutant Strains; D015398:Signal Transduction; D013601:T-Lymphocytes / Q000276:immunology*; D018418:Th2 Cells / Q000276:immunology
D018793:Interleukin-13; D015847:Interleukin-4