pUb6060: a broad-host-range, DNA polymerase-I-independent ColE2-like plasmid.
A ColE2-like, cryptic plasmid, pUB6060, of 5.8 kb has been found in a clinical isolate of Plesiomonas shigelloides. The complete sequence of pUB6060 has been determined and reveals a number of interesting features about the plasmid. The ColE2-like replication locus is linked to a functional ColE1-like mobilization locus. Replication is, unusually for ColE2 replicons, DNA polymerase-I-independent and may involve two, rather than the usual one, plasmid-encoded functions. Additionally, it carries two ORFs encoding products of unknown function. The pUB6060 replicon maintains a moderate plasmid copy number (10 per chromosome copy) and permits replication in diverse gram-negative bacteria.
Avison
M B
MB
Department of Pathology and Microbiology, Bristol Centre for Antimicrobial Research and Evaluation, Bristol, BS8 1TD, United Kingdom. Avison@bris.ac.uk
Walsh
T R
TR
Bennett
P M
PM
D001483:Base Sequence; D004256:DNA Polymerase I; D004261:DNA Replication; D004269:DNA, Bacterial / Q000502:physiology; D018628:Gene Dosage; D008969:Molecular Sequence Data; D010957:Plasmids / Q000502:physiology; D016981:Plesiomonas / Q000235:genetics*; D012093:Replicon / Q000502:physiology
D004269:DNA, Bacterial; D004256:DNA Polymerase I