Determination of aloesin in rat plasma using a column-switching high-performance liquid chromatographic assay.
A column-switching high-performance liquid chromatography (HPLC) method for the determination of aloesin in rat plasma using column-switching and ultraviolet (UV) absorbance detection is described. Plasma was directly injected onto the HPLC system consisting of a clean-up column, a concentrating column, and an analytical column, which were connected with a six-port switching valve. The determination of aloesin was accurate and repeatable, with a limit of quantitation of 10 ng/ml in plasma. The standard calibration curve for aloesin was linear (r=0.998) over the concentration range of 10-1000 ng/ml in rat plasma. The intra- and inter-day assay variabilities of aloesin ranged from 1.0 to 4.7% and 1.1 to 8.8%, respectively. This highly sensitive and simple method has been successfully applied to a pharmacokinetic study after oral administration of aloesin to rats.
Baek
M
M
Bioanalysis and Biotransformation Research Center, Korea Institute of Science and Technology, Seoul, South Korea.
Jeong
J H
JH
Kim
D H
DH
D000284:Administration, Oral; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D002867:Chromones / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D005960:Glucosides / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D015394:Molecular Structure; D051381:Rats; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D002867:Chromones; D005960:Glucosides; C069868:aloesin