Genomic comparison of an avirulent strain of Orf virus with that of a virulent wild type isolate reveals that the Orf virus G2L gene is non-essential for replication.
The genomic structure of two strains of orf virus (OV), a field isolate (MRI-Scab) which has never been passaged in cell culture, and a multiple-passage cell culture-adapted strain (Orf-11) were compared. The Orf-11 genome is approximately 8.0 kb longer than that of the MRI-Scab due to a duplication of the right-hand end. The duplicated region has been translocated to the left-hand end of the genome with a loss of sequence from that end. The lost sequence contains three complete genes, namely E2L, E3L and G1L and 80% of a fourth gene, namely G2L. The sequence lost from G2L in Orf-11 has been replaced by a region of unrelated sequence, encoding 98 amino acids. Northern analysis shows that mRNA is expressed from this "new" gene. The two viruses were also compared for in vivo virulence and ability to protect against subsequent OV challenge. In vivo, the field isolate was fully virulent and conferred good protection against challenge, whereas the cell culture-adapted virus produced only mild lesions and reduced protection against challenge.
McInnes
C J
CJ
Moredun Research Institute, Edinburgh, UK. mcinc@mri.sari.ac.uk
Wood
A R
AR
Nettleton
P E
PE
Gilray
J A
JA
D000595:Amino Acid Sequence; D000818:Animals; D002874:Chromosome Mapping; D004262:DNA Restriction Enzymes; D005814:Genes, Viral; D016679:Genome, Viral; D008969:Molecular Sequence Data; D009923:Orf virus / Q000235:genetics* / Q000472:pathogenicity; D015183:Restriction Mapping; D012756:Sheep; D014158:Transcription, Genetic; D014774:Virulence; D014779:Virus Replication / Q000235:genetics*
D004262:DNA Restriction Enzymes