Ultrastructural and functional changes in the jejunal epithelium of spontaneously hypertensive rats.
Ultrastructural studies on the epithelium, sugar transport and immunocytochemistry of Na+-glucose cotransporter (SGLT1) were carried out in the jejunum of Spontaneously Hypertensive Rats (SHR) and their normotensive genetic control, Wistar-Kyoto (WKY) rats. Electron microscopy studies showed a regular brush-border membrane in the jejunal enterocytes of WKY rats, with colloidal gold particles, representing SGLTI, localized at the microvilli of the absorptive epithelial cells. However, a patchy loss of microvilli was detected in the jejunal sections from SHR, with no presence of colloidal gold particles, indicating the absence of the SGLT1 protein. Most adjacent microvilli were normal in size like those found in WKY rats, and SGLT1 labeling was observed. All these changes were accompanied by a reduction in Na+-dependent D-glucose and D-galactose uptakes in the jejunal BBMVs isolated from SHR, when compared to WKY rats. We conclude that ultrastructural changes were paralleled by modifications in the sugar transport and in the localization of SGLT1 in the jejunal epithelium of SHR.
Sánchez-Aguayo
I
I
Department of Cellular Biology, Faculty of Biology, University of Seville, Sevilla, Spain.
Torreblanca
J
J
de La Hermosa
M L
ML
Mate
A
A
Planas
J M
JM
Vázquez
C M
CM
D000818:Animals; D001692:Biological Transport; D001794:Blood Pressure / Q000502:physiology; D020895:Enterocytes / Q000378:metabolism / Q000648:ultrastructure*; D005690:Galactose / Q000493:pharmacokinetics; D005947:Glucose / Q000493:pharmacokinetics; D006973:Hypertension / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000502:physiology / Q000648:ultrastructure*; D007583:Jejunum / Q000378:metabolism / Q000648:ultrastructure*; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism*; D008871:Microvilli / Q000378:metabolism / Q000648:ultrastructure; D009004:Monosaccharide Transport Proteins / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D051269:Sodium-Glucose Transporter 1
D008562:Membrane Glycoproteins; D009004:Monosaccharide Transport Proteins; C495676:Slc5a1 protein, rat; D051269:Sodium-Glucose Transporter 1; D005947:Glucose; D005690:Galactose