cDNA sequence and gene expression of storage protein-2--a juvenile hormone-suppressible hexamerin from the fall webworm, Hyphantria cunea Drury.
We isolated and sequenced a cDNA clone corresponding to storage protein-2 (SP-2) from the fall webworm, Hyphantria cunea. The cDNA for SP-2 (2572 bp) codes for a 747-residue protein with a predicted molecular mass of 88.5 kDa. The calculated isoelectric point is 7.6. Multiple alignment analysis of amino acid sequence revealed that SP-2 is most similar to BJHSP2 (74.3% identity). According to both the phylogenetic analyses and criteria for amino acid composition, SP-2 belongs to the subfamily of moderately methionine-rich storage proteins (3.2% methionine, 11.8% aromatic amino acid). Topical application of the JH analog, methoprene, after head ligation of larvae, suppressed transcription of the SP-2 gene, indicating hormonal effects at the transcriptional level. The SP-2 transcript was detected by Northern blot analysis in Malpighian tubules, in addition to the fat body where it was most abundant. The local expression of SP-2 in Malpighian tubules suggests that it may have some function in that organ.
Hwang
S J
SJ
Division of Life Science, College of Natural Sciences, Gyeongsang National University, 660-707, Chinju, South Korea.
Cheon
H M
HM
Kim
H J
HJ
Chae
K S
KS
Chung
D H
DH
Kim
M O
MO
Park
J S
JS
Seo
S J
SJ
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry; D000818:Animals; D015152:Blotting, Northern; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism; D015723:Gene Library; D019476:Insect Proteins / Q000096:biosynthesis* / Q000235:genetics*; D007915:Lepidoptera / Q000378:metabolism*; D008715:Methionine / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution; D014158:Transcription, Genetic
D000596:Amino Acids; D018076:DNA, Complementary; D019476:Insect Proteins; C041137:storage proteins, Insecta; D008715:Methionine