title: Differential control of clustering of the sodium channels Na(v)1.2 and Na(v)1.6 at developing CNS nodes of Ranvier.
abstract: Na(v)1.6 is the main sodium channel isoform at adult nodes of Ranvier. Here, we show that Na(v)1.2 and its beta2 subunit, but not Na(v)1.6 or beta1, are clustered in developing central nervous system nodes and that clustering of Na(v)1.2 and Na(v)1.6 is differentially controlled. Oligodendrocyte-conditioned medium is sufficient to induce clustering of Na(v)1.2 alpha and beta2 subunits along central nervous system axons in vitro. This clustering is regulated by electrical activity and requires an intact actin cytoskeleton and synthesis of a non-sodium channel protein. Neither soluble- or contact-mediated glial signals induce clustering of Na(v)1.6 or beta1 in a nonmyelinating culture system. These data reveal that the sequential clustering of Na(v)1.2 and Na(v)1.6 channels is differentially controlled and suggest that myelination induces Na(v)1.6 clustering.
authors.affiliation: Department of Neurobiology, Stanford University School of Medicine, Stanford, CA 94305, USA.
authors.forename: M R
authors.initials: MR
authors.lastname: Kaplan
authors.forename: M H
authors.initials: MH
authors.lastname: Cho
authors.forename: E M
authors.initials: EM
authors.lastname: Ullian
authors.forename: L L
authors.initials: LL
authors.lastname: Isom
authors.forename: S R
authors.initials: SR
authors.lastname: Levinson
authors.forename: B A
authors.initials: BA
authors.lastname: Barres
mesh_terms: D000818:Animals; D001681:Biological Assay / Q000379:methods; D002454:Cell Differentiation / Q000502:physiology; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002478:Cells, Cultured / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D002490:Central Nervous System / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism; D003599:Cytoskeleton / Q000187:drug effects / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000187:drug effects; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D009836:Oligodendroglia / Q000166:cytology / Q000378:metabolism; D009900:Optic Nerve / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism; D020033:Protein Isoforms / Q000378:metabolism; D021381:Protein Transport / Q000187:drug effects / Q000502:physiology; D011901:Ranvier's Nodes / Q000378:metabolism* / Q000648:ultrastructure; D051381:Rats; D015222:Sodium Channels / Q000378:metabolism*
chemical_list: D009419:Nerve Tissue Proteins; D020033:Protein Isoforms; D015222:Sodium Channels