Purification and characterization of murine beta-nerve growth factor.
Beta-nerve growth factor (beta-NGF) is a trophic factor in the nervous system. We aimed to isolate and characterize this protein in view of its potential therapeutic use in neurodegenerative diseases. For purification a two-step ion-exchange procedure was followed. The characterization was performed using separation and immunological techniques, as well as a biological assay. These studies showed that the obtained protein consisted of a mixture of beta-NGF molecules, intact at their NH2-terminal extreme, and molecules which have lost the NH2-terminal octapeptide and exhibit modifications increasing its hydrophobicity. All these molecular species were recognized immunologically and showed biological activity.
Castellanos Ortega
M R
MR
International Center for Neurological Restoration, Havana, Cuba. mariar@neubas.sld.cu
Cruz Aguado
R
R
Lorigados Pedré
L
L
de la Cuétara Bernal
K
K
D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D007525:Isoelectric Focusing; D051379:Mice; D020932:Nerve Growth Factor / Q000737:chemistry / Q000302:isolation & purification*; D015203:Reproducibility of Results
D020932:Nerve Growth Factor