title: Two enzymes of diacylglyceryl-O-4'-(N,N,N,-trimethyl)homoserine biosynthesis are encoded by btaA and btaB in the purple bacterium Rhodobacter sphaeroides.
abstract: Betaine lipids are ether-linked, nonphosphorous glycerolipids that resemble the more commonly known phosphatidylcholine in overall structure. Betaine lipids are abundant in many eukaryotes such as nonseed plants, algae, fungi, and amoeba. Some of these organisms are entirely devoid of phosphatidylcholine and, instead, contain a betaine lipid such as diacylglyceryl-O-4'-(N,N,N,-trimethyl)homoserine. Recently, this lipid also was discovered in the photosynthetic purple bacterium Rhodobacter sphaeroides where it seems to replace phosphatidylcholine under phosphate-limiting growth conditions. This discovery provided the opportunity to study the biosynthesis of betaine lipids in a bacterial model system. Mutants of R. sphaeroides deficient in the biosynthesis of the betaine lipid were isolated, and two genes essential for this process, btaA and btaB, were identified. It is proposed that btaA encodes an S-adenosylmethionine:diacylglycerol 3-amino-3-carboxypropyl transferase and btaB an S-adenosylmethionine-dependent N-methyltransferase. Both enzymatic activities can account for all reactions of betaine lipid head group biosynthesis. Because the equivalent reactions have been proposed for different eukaryotes, it seems likely that orthologs of btaA/btaB may be present in other betaine lipid-containing organisms.
authors.affiliation: Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI 48824-1319, USA.
authors.forename: R M
authors.initials: RM
authors.lastname: Klug
authors.forename: C
authors.initials: C
authors.lastname: Benning
mesh_terms: D019881:Aminoacyltransferases / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins; D001483:Base Sequence; D017931:DNA Primers; D008780:Methyltransferases / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D009876:Operon; D016133:Polymerase Chain Reaction; D012242:Rhodobacter sphaeroides / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D014280:Triglycerides / Q000096:biosynthesis*
chemical_list: C021660:1(3),2-diacylglyceryl-3(1)-O-4'-(N,N,N-trimethyl)homoserine; D001426:Bacterial Proteins; D017931:DNA Primers; D014280:Triglycerides; C427491:BtaB protein, Rhodobacter sphaeroides; D008780:Methyltransferases; D019881:Aminoacyltransferases; C427490:BtaA protein, Rhodobacter sphaeroides