Efficient transfer of intact oligonucleotides into the nucleus of ligament scar fibroblasts by HVJ-cationic liposomes is correlated with effective antisense gene inhibition.
The efficacy of two different cationic liposomes, Lipofectin and hemagglutinating virus of Japan (HVJ)-cationic liposomes, on nuclear uptake of fluorescence-labeled phosphorothioate oligodeoxyribonucleotide (S-ODN) by ligament scar fibroblasts and suppression of decorin mRNA expression when antisense decorin S-ODN was transferred was investigated. There was no significant difference in nuclear uptake of fluorescent ODN between the two methods. However, only HVJ-cationic liposomes had a significant effect on suppression of decorin mRNA expression levels. To address the discrepancy, the molecular integrity of the transferred ODN in the cells was assessed by analysis of fluorescence resonance energy transfer (FRET) within double-fluorescence-labeled S-ODN. More than 70% of the ODN transfected by HVJ-cationic liposomes remained intact within the nucleus at 20 h after transfection, while the majority of the ODN transferred by Lipofectin was degraded at this point. These results suggest a strong relationship between the nuclear integrity of transfected antisense ODN and its suppression of target mRNA expression.
Nakamura
N
N
McCaig Centre for Joint Injury and Arthritis Research, University of Calgary, Alberta, Canada.
Hart
D A
DA
Frank
C B
CB
Marchuk
L L
LL
Shrive
N G
NG
Ota
N
N
Taira
K
K
Yoshikawa
H
H
Kaneda
Y
Y
D021581:Active Transport, Cell Nucleus; D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D058575:Decorin; D004337:Drug Carriers; D016326:Extracellular Matrix Proteins; D005347:Fibroblasts; D005452:Fluoresceins / Q000493:pharmacokinetics; D008055:Lipids / Q000737:chemistry*; D008081:Liposomes / Q000008:administration & dosage*; D020319:Oligodeoxyribonucleotides, Antisense / Q000493:pharmacokinetics*; D010714:Phosphatidylethanolamines / Q000008:administration & dosage*; D011509:Proteoglycans / Q000037:antagonists & inhibitors / Q000235:genetics; D012333:RNA, Messenger / Q000037:antagonists & inhibitors* / Q000235:genetics; D011817:Rabbits; D018108:Respirovirus / Q000737:chemistry*; D014018:Tissue Distribution
D058575:Decorin; D004337:Drug Carriers; D016326:Extracellular Matrix Proteins; D005452:Fluoresceins; D008055:Lipids; D008081:Liposomes; D020319:Oligodeoxyribonucleotides, Antisense; D010714:Phosphatidylethanolamines; D011509:Proteoglycans; D012333:RNA, Messenger; C024098:6-carboxyfluorescein; C020888:1,2-dielaidoylphosphatidylethanolamine