C terminal CYS-RICH region of mumps virus structural V protein correlates with block of interferon alpha and gamma signal transduction pathway through decrease of STAT 1-alpha.
It has been reported that interferon (IFN)-alpha/gamma signal transduction pathway is blocked in several cell lines persistently infected with mumps virus (MV) through decrease of STAT-1alpha. Expression of the MV structural V protein (MV-V) or C terminal CYS-RICH region of the V protein (MV-Vsp) inhibited the establishment of the antivirus state induced by IFN, but not by expression of the MV-P protein. Suppression of IFN-induced STAT-1alpha, STAT-2, and IRF-9 (p48) induction was also recognized in the cells transfected with expression vector of the MV-V (pTM-V) or MV-Vsp (pTM-Vsp) protein, even though it was in the absence of the other virus protein. It is supposed that the cysteine-rich domain of V protein (Vsp) is involved in the suppression of the IFN signal transduction pathway.
Kubota
T
T
Department of Microbiology, Sapporo Medical University School of Medicine, South 1, West 16, Chou-ku, Sapporo, Hokkaido, 060-8556, Japan.
Yokosawa
N
N
Yokota
S
S
Fujii
N
N
D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D002522:Chlorocebus aethiops; D002999:Clone Cells / Q000187:drug effects / Q000378:metabolism / Q000821:virology; D004268:DNA-Binding Proteins / Q000378:metabolism; D050832:Interferon-Stimulated Gene Factor 3; D016898:Interferon-alpha / Q000037:antagonists & inhibitors* / Q000378:metabolism / Q000494:pharmacology; D007371:Interferon-gamma / Q000037:antagonists & inhibitors* / Q000378:metabolism / Q000494:pharmacology; D009109:Mumps virus / Q000502:physiology; D010446:Peptide Fragments / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D050795:STAT2 Transcription Factor; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology*; D015534:Trans-Activators / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism*; D014162:Transfection; D014709:Vero Cells; D010948:Viral Plaque Assay; D015678:Viral Structural Proteins / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology
D004268:DNA-Binding Proteins; D050832:Interferon-Stimulated Gene Factor 3; D016898:Interferon-alpha; D010446:Peptide Fragments; D050795:STAT2 Transcription Factor; D015534:Trans-Activators; D014157:Transcription Factors; D015678:Viral Structural Proteins; C079868:gamma interferon activation factor; D007371:Interferon-gamma