title: Differential induction of peroxisomal populations in subcellular fractions of rat liver.
abstract: In rat liver, peroxisome proliferators induce profound changes in the number and protein composition of peroxisomes, which upon subcellular fractionation is reflected in heterogeneity in sedimentation properties of peroxisome populations. In this study we have investigated the time course of induction of the peroxisomal proteins catalase, acyl-CoA oxidase (ACO) and the 70 kDa peroxisomal membrane protein (PMP70) in different subcellular fractions. Rats were fed a di(2-ethylhexyl)phthalate (DEHP) containing diet for 8 days and livers were removed at different time-points, fractionated by differential centrifugation into nuclear, heavy and light mitochondrial, microsomal and soluble fractions, and organelle marker enzymes were measured. Catalase was enriched mainly in the light mitochondrial and soluble fractions, while ACO was enriched in the nuclear fraction (about 30%) and in the soluble fraction. PMP70 was found in all fractions except the soluble fraction. DEHP treatment induced ACO, catalase and PMP70 activity and immunoreactive protein, but the time course and extent of induction was markedly different in the various subcellular fractions. All three proteins were induced more rapidly in the nuclear fraction than in the light mitochondrial or microsomal fractions, with catalase and PMP70 being maximally induced in the nuclear fraction already at 2 days of treatment. Refeeding a normal diet quickly normalized most parameters. These results suggest that induction of a heavy peroxisomal compartment is an early event and that induction of 'small peroxisomes', containing PMP70 and ACO, is a late event. These data are compatible with a model where peroxisomes initially proliferate by growth of a heavy, possibly reticular-like, structure rather than formation of peroxisomes by division of pre-existing organelles into small peroxisomes that subsequently grow. The various peroxisome populations that can be separated by subcellular fractionation may represent peroxisomes at different stages of biogenesis.
authors.affiliation: The Wenner-Gren Institute, Stockholm University, Sweden. mona.wilcke@eu.pnu.com
authors.forename: M
authors.initials: M
authors.lastname: Wilcke
authors.forename: S E
authors.initials: SE
authors.lastname: Alexson
mesh_terms: D018528:ATP-Binding Cassette Transporters; D042962:Acyl-CoA Oxidase; D000818:Animals; D015153:Blotting, Western; D002374:Catalase / Q000378:metabolism; D004051:Diethylhexyl Phthalate / Q000494:pharmacology; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000648:ultrastructure*; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D010088:Oxidoreductases / Q000378:metabolism; D020675:Peroxisomes / Q000201:enzymology / Q000648:ultrastructure*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013347:Subcellular Fractions / Q000201:enzymology / Q000648:ultrastructure*
chemical_list: D018528:ATP-Binding Cassette Transporters; C489272:Abcd3 protein, rat; D008565:Membrane Proteins; D004051:Diethylhexyl Phthalate; D010088:Oxidoreductases; D002374:Catalase; D042962:Acyl-CoA Oxidase