title: Influence of mild hypothermia on myocardial contractility and circulatory function.
abstract: Myocardial contractility depends on temperature. We investigated the influence of mild hypothermia (37-31 degrees C) on isometric twitch force, sarcoplasmic reticulum (SR) Ca2+-content and intracellular Ca2+-transients in ventricular muscle strips from human and porcine myocardium, and on in vivo hemodynamic parameters in pigs. In vitro experiments: muscle strips from 5 nonfailing human and 8 pig hearts. Electrical stimulation (1 Hz), simultaneous recording of isometric force and rapid cooling contractures (RCCs) as an indicator of SR Ca2+-content, or intracellular Ca2+-transients (aequorin method). In vivo experiments: 8 pigs were monitored with Millar-Tip (left ventricle) and Swan-Ganz catheter (pulmonary artery). Hemodynamic parameters were assessed at baseline conditions (37 degrees C), and after stepwise cooling on cardiopulmonary bypass to 35, 33 and 31 degrees C. Hypothermia increased isometric twitch force significantly by 91 +/- 16 % in human and by 50 +/- 9 % in pig myocardium (31 vs. 37 degrees C; p < 0.05, respectively). RCCs or aequorin light emission did not change significantly. In anesthetized pigs, mild hypothermia resulted in an increase in hemodynamic parameters of myocardial contractility. While heart rate decreased from 111 +/- 3 to 73 +/- 1 min(-1), cardiac output increased from 2.4 +/- 0.1 to 3.1 +/- 0.31/min, and stroke volume increased from 21 +/- 1 to 41 +/- 3 ml. +dP/dtmax increased by 25 +/- 8% (37 vs. 31 degrees C; p < 0.05 for all values). Systemic and pulmonary vascular resistance did not change significantly during cooling. Mild hypothermia exerts significant positive inotropic effects in human and porcine myocardium without increasing intracellular Ca2+-transients or SR Ca2+-content. These effects translate into improved hemodynamic parameters of left ventricular function.
authors.affiliation: Abteilung Kardiologie und Pneumologie, Zentrum Innere Medizin, Georg-August-Universität Göttingen, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Weisser
authors.forename: J
authors.initials: J
authors.lastname: Martin
authors.forename: E
authors.initials: E
authors.lastname: Bisping
authors.forename: L S
authors.initials: LS
authors.lastname: Maier
authors.forename: F
authors.initials: F
authors.lastname: Beyersdorf
authors.forename: G
authors.initials: G
authors.lastname: Hasenfuss
authors.forename: B
authors.initials: B
authors.lastname: Pieske
mesh_terms: D000331:Aequorin; D000818:Animals; D002118:Calcium / Q000378:metabolism; D003326:Coronary Circulation / Q000502:physiology*; D006333:Heart Failure / Q000503:physiopathology / Q000628:therapy; D007036:Hypothermia, Induced; D066298:In Vitro Techniques; D007537:Isometric Contraction / Q000502:physiology; D009200:Myocardial Contraction / Q000502:physiology*; D013318:Stroke Volume / Q000502:physiology; D013552:Swine; D014655:Vascular Resistance / Q000502:physiology; D016277:Ventricular Function, Left / Q000502:physiology*; D017725:Ventricular Pressure / Q000502:physiology
chemical_list: D000331:Aequorin; D002118:Calcium