[Study of antiproliferative effect of gamma-interferon and heparin on rabbit lens epithelial cell (RLEC) cultured in vitro].
OBJECTIVE
To study the antiproliferative effect of gamma-interferon and heparin on rabbit lens epithelial cell(RLEC).


METHODS
First passage RLEC was obtained from healthy New zealand white rabbits. Third passage cells were incubated with gamma-interferon with concentration from 0.1 u/ml to 10,000 u/ml and heparin with concentration from 100-5,000 u/ml for 48 hours. The Mosmann's MTT colorimeltric assay was used to quantitate the RLEC proliferative activation.


RESULTS
In lower concentration groups (0.1-100 u/ml), gamma-interferon had no antiproliferative effect on RLEC, while in higher concentration groups (1,000 u/ml-10,000 u/ml) it exhibited some antiproliferative effect on RLEC, the rate of inhibition were 27% and 38%, respectively. Heparin of all experimental groups had antiproliferative effect on RLEC.


CONCLUSIONS
gamma-interferon and heparin with certain concentration can inhibit proliferation of RLEC directly.
Wang
J
J
Zhonghsan Ophthalmic Center, Sun Yat-sen University of Medical Sciences, Guangzhou 510060, China.
Li
S
S
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000187:drug effects; D005260:Female; D006493:Heparin / Q000494:pharmacology*; D007371:Interferon-gamma / Q000494:pharmacology*; D007908:Lens, Crystalline / Q000166:cytology* / Q000187:drug effects; D008297:Male; D011817:Rabbits; D011994:Recombinant Proteins
D011994:Recombinant Proteins; D007371:Interferon-gamma; D006493:Heparin