Differential palmitoylation of two mouse glutamate receptor interacting protein 1 forms with different N-terminal sequences.
Glutamate receptor interacting protein (GRIP) is a member of the PDZ domain-containing protein family that is localized in the postsynaptic density area. This protein has been reported to interact specifically with the C-termini of AMPA-selective glutamate receptor channel subunits, GluRalpha2 and GluRalpha3 through its PDZ domains. To clarify the physiological functions of GRIP, we cloned mouse GRIP1, and found that there are three sites for alternative splicing and two putative translational start codons by characterizing GRIP1 cDNA clones and reverse transcription-polymerase chain reaction products. Metabolic labeling of COS-7 cells expressing two N-terminal GRIP1 proteins demonstrated that these proteins differed in their pattern of palmitoylation. These findings suggested that the molecular diversity of GRIP1 underlies the localization and functional heterogeneity of this protein.
Yamazaki
M
M
Department of Cellular Neurobiology, Brain Research Institute, Niigata University, 1-757 Asahimachi-dori, 951-8585, Niigata, Japan.
Fukaya
M
M
Abe
M
M
Ikeno
K
K
Kakizaki
T
T
Watanabe
M
M
Sakimura
K
K
D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D000831:Animals, Newborn; D019556:COS Cells / Q000378:metabolism; D002352:Carrier Proteins / Q000378:metabolism*; D002531:Cerebellum / Q000254:growth & development / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000378:metabolism*; D015723:Gene Library; D051379:Mice; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D019308:Palmitic Acid / Q000378:metabolism*; D016548:Prosencephalon / Q000254:growth & development / Q000378:metabolism*; D022821:RNA Splice Sites / Q000502:physiology; D018091:Receptors, AMPA / Q000378:metabolism
D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; D004791:Enzyme Inhibitors; C491309:Grip1 protein, mouse; D009419:Nerve Tissue Proteins; D022821:RNA Splice Sites; D018091:Receptors, AMPA; D019308:Palmitic Acid