title: Effects of storage and type of blood collection tubes on hepatitis C virus level in whole blood samples.
abstract: In this study, we compared serum hepatitis C virus (HCV) RNA concentrations with HCV RNA concentrations in whole blood collection tubes, including two different types of EDTA tubes and nucleic acid stabilization tubes (NASTs). We also investigated the impact of a processing delay on HCV RNA concentration in these tubes. In NASTs, the mean HCV RNA concentration was comparable to the mean serum HCV RNA concentration at "date zero." In EDTA tubes, mean baseline HCV RNA concentrations were higher. Storage at room temperature up to 96 h did not result in a decline of HCV RNA concentration in any of the whole blood collection tubes. In NASTs, HCV RNA concentrations remained stable during the whole study period, whereas a significant increase of HCV RNA was observed in both types of EDTA tubes at 96 h compared to date zero. We concluded that HCV RNA remains stable in NASTs at room temperature for at least 96 h, allowing greater flexibility in sample collection and transport.
authors.affiliation: Molecular Diagnostics Laboratory, Institute of Hygiene, Karl-Franzens-University Graz, Universitaetsplatz 4, A-8010 Graz, Austria. harald.kessler@uni-graz.at
authors.forename: H H
authors.initials: HH
authors.lastname: Kessler
authors.forename: E
authors.initials: E
authors.lastname: Stelzl
authors.forename: R B
authors.initials: RB
authors.lastname: Raggam
authors.forename: J
authors.initials: J
authors.lastname: Haas
authors.forename: F
authors.initials: F
authors.lastname: Kirchmeir
authors.forename: K
authors.initials: K
authors.lastname: Hegenbarth
authors.forename: E
authors.initials: E
authors.lastname: Daghofer
authors.forename: B I
authors.initials: BI
authors.lastname: Santner
authors.forename: E
authors.initials: E
authors.lastname: Marth
authors.forename: R E
authors.initials: RE
authors.lastname: Stauber
mesh_terms: D000328:Adult; D001769:Blood / Q000821:virology*; D001800:Blood Specimen Collection / Q000295:instrumentation / Q000379:methods; D005260:Female; D016174:Hepacivirus / Q000302:isolation & purification*; D006526:Hepatitis C / Q000821:virology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D012367:RNA, Viral / Q000097:blood*
chemical_list: D012367:RNA, Viral