Chemical force microscopy with active enzymes.
The adhesion forces have been measured between an atomic force microscope tip derivatized with an active enzyme, shikimate kinase, and an ATP mimic immobilized on a gold surface. Experiments with competitive binding of other ligands in solution show that the observed adhesion forces arise predominantly from specific interactions between the immobilized enzyme and surface-bound adenine derivative. These experiments represent a step in the development of a screening methodology based upon chemical force microscopy.
Fiorini
M
M
Department of Chemistry, University of Cambridge, Cambridge CB2 1EW, United Kingdom.
McKendry
R
R
Cooper
M A
MA
Rayment
T
T
Abell
C
C
D001667:Binding, Competitive; D002384:Catalysis; D002448:Cell Adhesion; D004798:Enzymes / Q000737:chemistry*; D006046:Gold / Q000737:chemistry; D007700:Kinetics; D008024:Ligands; D018625:Microscopy, Atomic Force / Q000379:methods*; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000737:chemistry; D011485:Protein Binding; D012765:Shikimic Acid / Q000378:metabolism; D013055:Spectrophotometry, Infrared; D020349:Surface Plasmon Resonance; D013997:Time Factors
D004798:Enzymes; D008024:Ligands; D012765:Shikimic Acid; D006046:Gold; D017853:Phosphotransferases (Alcohol Group Acceptor); C022642:shikimate kinase