Developmental expression of mouse Krüppel-like transcription factor KLF7 suggests a potential role in neurogenesis.
To identify potential functions for the Krüppel-like transcription factor KLF7, we have determined the spatiotemporal pattern of gene expression during embryogenesis and in the adult organism. We show that the profile of Klf7 expression predominantly involves the central and peripheral nervous systems and is broadly identified by three separate phases. The first phase occurs early in embryogenesis with increasingly strong expression in the spinal cord, notably in motor neurons of the ventral horn, in dorsal root ganglia, and in sympathetic ganglia. The second robust phase of Klf7 expression is confined to the early postnatal cerebral cortex and is downregulated thereafter. The third phase is characterized by high and sustained expression in the adult cerebellum and dorsal root ganglia. Functionally, these three phases coincide with establishment of neuronal phenotype in embryonic spinal cord, with synaptogenesis and development of mature synaptic circuitry in the postnatal cerebral cortex, and with survival and/or maintenance of function of adult sensory neurons and cerebellar granule cells. Consistent with Klf7 expression in newly formed neuroblasts, overexpression of the gene in cultured fibroblasts and neuroblastoma cells repressed cyclin D1, activated p21, and led to G1 growth arrest. Based on these data, we argue for multiple potential functions for KLF7 in the developing and adult nervous system; they include participating in differentiation and maturation of several neuronal subtypes and in phenotypic maintenance of mature cerebellar granule cells and dorsal root ganglia.
Laub
F
F
Brookdale Center, Department of Biochemistry and Molecular Biology, Mount Sinai School of Medicine--New York University, One Gustave L. Levy Place, New York, New York 10029, USA.
Aldabe
R
R
Friedrich
V
V
Ohnishi
S
S
Yoshida
T
T
Ramirez
F
F
D000595:Amino Acid Sequence; D000818:Animals; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental; D017404:In Situ Hybridization, Fluorescence; D051741:Kruppel-Like Transcription Factors; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009420:Nervous System / Q000196:embryology* / Q000254:growth & development; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution; D014157:Transcription Factors / Q000235:genetics* / Q000502:physiology*; D014162:Transfection
D004268:DNA-Binding Proteins; C426008:Klf7 protein, mouse; D051741:Kruppel-Like Transcription Factors; D014157:Transcription Factors