A Rsc3/Rsc30 zinc cluster dimer reveals novel roles for the chromatin remodeler RSC in gene expression and cell cycle control.
Chromatin remodeling complexes perform central roles in transcriptional regulation. Here, we identify Rsc3 and Rsc30 as novel components of the essential yeast remodeler RSC complex. Rsc3 and Rsc30 function requires their zinc cluster domain, a known site-specific DNA binding motif. RSC3 is essential, and rsc3 Ts- mutants display a G2/M cell cycle arrest involving the spindle assembly checkpoint pathway, whereas rsc30Delta mutants are viable and osmosensitive. Rsc3 and Rsc30 interact functionally and also physically as a stable Rsc3/Rsc30 heteromeric complex. However, DNA microarray analysis with rsc3 or rsc30 mutants reveals different effects on the expression levels of ribosomal protein genes and cell wall genes. We propose that Rsc3 and Rsc30 interact physically but have different roles in targeting or regulating RSC.
Angus-Hill
M L
ML
Howard Hughes Medical Institute, University of Utah School of Medicine, Salt Lake City, UT 84108, USA.
Schlichter
A
A
Roberts
D
D
Erdjument-Bromage
H
H
Tempst
P
P
Cairns
B R
BR
D001483:Base Sequence; D002453:Cell Cycle / Q000235:genetics; D002473:Cell Wall / Q000502:physiology; D002843:Chromatin / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D019281:Dimerization; D016195:G2 Phase / Q000235:genetics; D015966:Gene Expression Regulation, Fungal; D005804:Genes, Lethal / Q000502:physiology; D018816:Genes, cdc / Q000502:physiology; D016350:Leucine Zippers / Q000502:physiology; D008938:Mitosis / Q000235:genetics; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009687:Nuclear Proteins / Q000235:genetics; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D012269:Ribosomal Proteins / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D008941:Spindle Apparatus / Q000235:genetics / Q000378:metabolism; D013696:Temperature; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D015003:Yeasts; D015032:Zinc / Q000737:chemistry / Q000378:metabolism*
D002843:Chromatin; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; C097698:PAF1 protein, S cerevisiae; C103337:RSC complex, S cerevisiae; D012269:Ribosomal Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D015032:Zinc