Functionalized biopolymers as soluble macromolecular chelating agents.
Two different conjugates of bovine serum albumin (BSA) with lysine and a derivative of imidazole have been synthesized to obtain watersoluble macromolecules with binding properties against bivalent transition metal ions. Syntheses have been carried out using the 60 aminogroups or the 99 carboxylic groups on BSA for the coupling reactions, with such molar ratios able to produce highly substituted BSA. The skill of each conjugate to bind metal ions in aqueous medium was studied through the use of titration curves with some metal ions, characterized by a good affinity for the free ligand. Both the conjugates allow us to recover a high number of metal ions per protein molecule, close to the number of ligand molecules on the BSA surface in the case of the lysine conjugate, whereas in the case of the imidazole conjugate M3L complexes are performed.
Giraudi
G
G
Dipartimento di Chimica Analitica, Università di Torino Via P. Giuria 5, 10125 Turin, Italy. giraudi@ch.unito.it
Baggiani
C
C
Giovannoli
C
C
Anfossi
L
L
Tozzi
C
C
Vanni
A
A
D001704:Biopolymers / Q000737:chemistry*; D002614:Chelating Agents / Q000138:chemical synthesis*; D006632:Histamine / Q000737:chemistry; D008239:Lysine / Q000737:chemistry; D019216:Metals, Heavy / Q000032:analysis / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D012995:Solubility; D013056:Spectrophotometry, Ultraviolet
D001704:Biopolymers; D002614:Chelating Agents; D019216:Metals, Heavy; D012710:Serum Albumin, Bovine; D006632:Histamine; D008239:Lysine