title: Does blood of healthy subjects contain bacterial ribosomal DNA?
abstract: Real-time PCR methods with primers and a probe targeting conserved regions of the bacterial 16S ribosomal DNA (rDNA) revealed a larger amount of rDNA in blood specimens from healthy individuals than in matched reagent controls. However, the origins and identities of these blood-associated bacterial rDNA sequences remain obscure.
authors.affiliation: Department of Microbiology and Immunology and Department of Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA. snikkari@cmgm.stanford.edu
authors.forename: S
authors.initials: S
authors.lastname: Nikkari
authors.forename: I J
authors.initials: IJ
authors.lastname: McLaughlin
authors.forename: W
authors.initials: W
authors.lastname: Bi
authors.forename: D E
authors.initials: DE
authors.lastname: Dodge
authors.forename: D A
authors.initials: DA
authors.lastname: Relman
mesh_terms: D001419:Bacteria / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000097:blood*; D004275:DNA, Ribosomal / Q000097:blood*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S