title: Stirrer tank: an appropriate technology to immobilize the CB.Hep-1 monoclonal antibody for immunoaffinity purification.
abstract: The CB.Hep-1 monoclonal antibody was coupled to CNBr-activated Sepharose CL 4B at three different immobilization scales for purification of recombinant hepatitis B surface antigen. Standard laboratory apparatus to obtain immunosorbents of 1 l (scale I) and 3 l (scale II) as well as a stirrer tank to prepare 6 l immunosorbents (scale III) were used. The binding capacity at scale III was 2- and 1.5-fold higher with respect to the scales II and I, while a reduction in the ligand leakage of 5- and 2-folds was observed. Immunosorbents from scale II showed a significantly reduced adsorption, and an increased ligand leakage. Differences in the coupling efficiency were not observed. Antigen purity eluted from the immunosorbents was always above 85%.
authors.affiliation: Monoclonal Antibody Division, Center for Genetic Engineering and Biotechnology, Havana, Cuba. raul.hernandez@cigb.edu.cu
authors.forename: R
authors.initials: R
authors.lastname: Hernández
authors.forename: E
authors.initials: E
authors.lastname: Chong
authors.forename: R
authors.initials: R
authors.lastname: Morales
authors.forename: E
authors.initials: E
authors.lastname: Pérez
authors.forename: Y
authors.initials: Y
authors.lastname: Amador
authors.forename: J R
authors.initials: JR
authors.lastname: Zubiaurrez
authors.forename: R
authors.initials: R
authors.lastname: Valdés
authors.forename: A
authors.initials: A
authors.lastname: Figueroa
authors.forename: A
authors.initials: A
authors.lastname: Agraz
authors.forename: L
authors.initials: L
authors.lastname: Herrera
mesh_terms: D000911:Antibodies, Monoclonal; D006514:Hepatitis B Surface Antigens / Q000276:immunology / Q000302:isolation & purification*; D007163:Immunosorbent Techniques / Q000295:instrumentation*
chemical_list: D000911:Antibodies, Monoclonal; D006514:Hepatitis B Surface Antigens