A linkage map for CRINKLED PETAL: a homeotic gene of Clarkia tembloriensis (Onagraceae).
Homeotic mutations in flowers lead to the development of floral organs in abnormal locations. In most laboratory-induced examples of this type of mutation, two adjacent whorls of organs are affected, resulting in two whorls of abnormal organ formation. However, the crinkled petal mutant of Clarkia tembloriensis is interesting because it is a naturally occurring mutation and it affects only the second whorl of organs, producing sepaloid petals. In this study one wild-type population (Cantua Creek-2) and one crinkled petal mutant population (Red Rocks) were compared using 181 different primers in random amplified polymorphic DNA (RAPD) analysis. Bulk DNA from each parent population and their subsequent crosses were used to compare the genetic differences between the two populations and to search for molecular markers linked with the CRINKLED PETAL locus. A linkage map was developed for the CRINKLED PETAL gene, and markers were discovered which flanked both sides of the locus.
Leong
L L
LL
Department of Botany, Miami University, Oxford, Ohio 45056, USA. Leongll@aol.com
Wilson
K G
KG
Smith-Huerta
N L
NL
D002874:Chromosome Mapping / Q000379:methods*; D005801:Genes, Homeobox; D017343:Genes, Plant; D008040:Genetic Linkage; D010641:Phenotype; D010944:Plants / Q000235:genetics*; D019105:Random Amplified Polymorphic DNA Technique