[Determination of uric acid, creatinine and pseudouridine in human urine by high performance capillary zone electrophoresis].
A simultaneous determination of uric acid, creatinine and pseudouridine in human urine by high performance capillary zone electrophoresis(HPCZE) is described. The urine samples were refrigerated and filtered. Then the samples were directly introduced into the capillary and a phosphate buffer solution(pH 6.1) was employed. The capillary used was 36 cm x 50 microns i.d. and detection was carried out with a UV monitor at 210 nm. The calibration curve showed a good linearity for uric acid, creatinine, and pseudouridine in the concentration range of 2-80 mg/L and their correlation coefficients were 0.996, 0.996 and 0.999 respectively. Within day CV for assaying the urine samples of uric acid, creatinine and pseudouridine were 5.0%, 3.0%, and 4.4%, and the corresponding between day data were 6.5%, 5.9%, and 5.3%, respectively. The recoveries of uric acid, creatinine and pseudouridine were 99.0%, 102.%, 92.2%, respectively. The total time for separation and determination was within 10 min. This method is characterized by direct assay of urine samples without any pretreatment. The results show that HPCZE is a simple, rapid, sensitive and reliable assay method.
Zhou
J
J
General Hospital of Nanjing Command, PLA, Nanjing, 210002.
Yuan
Y
Y
Xu
D
D
D003404:Creatinine / Q000652:urine*; D019075:Electrophoresis, Capillary / Q000379:methods; D006801:Humans; D011560:Pseudouridine / Q000652:urine*; D014527:Uric Acid / Q000652:urine*
D011560:Pseudouridine; D014527:Uric Acid; D003404:Creatinine