A comparison of routine and rapid microwave tissue processing in a surgical pathology laboratory. Quality of histologic sections and advantages of microwave processing.
Rapid processing of histopathologic material is becoming increasingly desirable to fulfill the needs of clinicians treating acutely ill patients. Traditional techniques for rapid processing of paraffin-embedded tissues require 4 to 5 hours, delaying treatment for some critically ill patients and requiring additional shifts of technologists in the laboratory. Microwave processing further shortens this time, allowing even more rapid histopathologic diagnosis. Few data exist comparing quality of microwave-processed tissue with that processed by more traditional techniques. We randomly selected 158 paired specimens from 111 patients. One member of the pair was processed routinely overnight, while the other was processed by the rapid microwave technique. The slides then were compared for quality of histologic preparation in a blinded fashion by 2 pathologists. Eight routinely processed specimens were judged as suboptimal, while 6 microwave-processed specimens were judged as suboptimal and 1 was considered unsatisfactory for evaluation. In the remaining cases, the material obtained by the 2 techniques was considered of identical quality. Microwave processing considerably shortens the preparation time for permanent histologic sections without a demonstrable decrease in section quality or "readability."
Rohr
L R
LR
Department of Pathology, University of Utah, Salt Lake City, and Associated Regional and University Pathologists (ARUP), Salt Lake City, USA.
Layfield
L J
LJ
Wallin
D
D
Hardy
D
D
D005260:Female; D016591:Histocytological Preparation Techniques / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D008297:Male; D008872:Microwaves; D016612:Paraffin Embedding / Q000379:methods; D010340:Pathology, Surgical / Q000295:instrumentation / Q000379:methods*; D013997:Time Factors; D014021:Tissue Preservation / Q000379:methods