Simultaneous high-performance liquid chromatographic determination of urinary mandelic and phenylglyoxylic acids as indirect evaluation of styrene exposure.
Styrene is rapidly metabolised to mandelic acid (MA) and phenylglyoxylic acid (PGA), which are excreted in urine. In this work, we have developed a simple, sensitive and specific high-performance liquid chromatographic method with minor sample preparation procedures for the simultaneous determination of MA and PGA in urine of workers exposed to styrene. Moreover, urine samples from workers of two plastic factories were analysed, styrene exposure levels of the workers were estimated and data obtained from the two factories were compared.
Laffon
B
B
Departmento de Biología Celular y Molecular, Facultad de Ciencias, Universidade da Coruña, Spain.
Lema
M
M
Méndez
J
J
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006038:Glyoxylates / Q000652:urine*; D006801:Humans; D008333:Mandelic Acids / Q000652:urine*; D016273:Occupational Exposure; D012680:Sensitivity and Specificity; D020058:Styrene / Q000009:adverse effects*
D006038:Glyoxylates; D008333:Mandelic Acids; C012482:phenylglyoxylic acid; D020058:Styrene; C037938:mandelic acid