Oxygen free radical production in rat liver: dose-related effect of ethanol on reperfusion injury.
Ethanol is known to have a deleterious effect on liver ischemia-reperfusion injury, but recent reports suggest that light ethanol consumption may produce a protective effect in several organs. We aimed to investigate effects of different doses of ethanol on liver oxidative injury. Rats were fed with ethanol-containing diets (24, 30, 36, 40% for groups A, B, C, D, respectively). After four weeks, livers were exposed to ischemia-reperfusion. Chemiluminescence was recorded; total lipids, adenosine triphosphate, malondialdehyde, reduced glutathione and lactic dehydrogenase were assessed. In all groups, ischemia resulted in the disappearance of O2*-, a decrease in glutathione and adenosine triphosphate, and stable malondialdehyde values. During the reperfusion phase, O2*- production, malondialdehyde and lactic dehydrogenase increased, reaching significantly higher values in groups C and D and significantly lower values in group B. The effect of ethanol on ischemia-reperfusion injury seems to be a dose-related response, with an additional toxic effect only at high doses of ethanol.
Addolorato
G
G
Patologia Medica and Medicina Interna, Catholic University of Rome, Italy.
Di Campli
C
C
Simoncini
M
M
Pasini
P
P
Nardo
B
B
Cavallari
A
A
Pola
P
P
Roda
A
A
Gasbarrini
G
G
Gasbarrini
A
A
D000255:Adenosine Triphosphate / Q000032:analysis; D000818:Animals; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000008:administration & dosage*; D005609:Free Radicals / Q000378:metabolism; D005978:Glutathione / Q000032:analysis; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D008055:Lipids / Q000032:analysis; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008163:Luminescent Measurements; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D010100:Oxygen / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000378:metabolism*
D005609:Free Radicals; D008055:Lipids; D000431:Ethanol; D008315:Malondialdehyde; D000255:Adenosine Triphosphate; D007770:L-Lactate Dehydrogenase; D005978:Glutathione; D010100:Oxygen