title: LC-NMR: a new tool to expedite the dereplication and identification of natural products.
abstract: The rapid identification of known or undesirable compounds from natural products extracts - "dereplication" - is an important step in an efficiently run natural products discovery program. Dereplication strategies use analytical techniques and database searching to determine the identity of an active compound at the earliest possible stage in the discovery process. In the past few years, advances in technology have allowed the development of tandem analytical techniques such as liquid chromatography mass spectrometry (LC-MS), LC-MS-MS, liquid chromatography nuclear magnetic resonance (LC-NMR), and LC-NMR-MS. LC-NMR, despite its lower sensitivity as compared to LC-MS, provides a powerful tool for rapid identification of known compounds and identification of structure classes of novel compounds. LC-NMR is especially useful in instances where the data from LC-MS are incomplete or do not allow confident identification of the active component of a sample. LC-NMR has been used to identify the marine alkaloid aaptamine as the active component in an extract of the sponge Aaptos sp. This extract had been identified as an enzyme inhibitor by a high throughput screening (HTS) effort. Isolated aaptamine exhibited an IC(50)=120 µM against this enzyme. Strategies for the identification of aaptamine and for the use of LC-NMR in a natural products HTS program are discussed.
authors.affiliation: Monsanto Nutrition and Consumer Products, Monsanto Company, St. Louis, MO 63167.
authors.forename: S C
authors.initials: SC
authors.lastname: Bobzin
authors.forename: S
authors.initials: S
authors.lastname: Yang
authors.forename: T P
authors.initials: TP
authors.lastname: Kasten