Measurement of fecal sulfide using gas chromatography and a sulfur chemiluminescence detector.
We describe a simple technique to measure sulfide in fecal homogenates (or any other liquid milieu), which involves acidification followed by the G.C. measurement of H2S in a gas space equilibrated with a small quantity of homogenate. An internal standard of Zn35S added to the homogenate permits correction for incomplete recovery of H2S in the gas space. The use of a sulfur chemiluminescence detector, which specifically and sensitively responds to sulfur-containing compounds, greatly facilitates this measurement.
Fume
J K
JK
Department of Veterans Affairs, Medical Centre, Minneapolis, MN 55417, USA.
Springfield
J
J
Koenig
T
T
Suarez
F
F
Levitt
M D
MD
D002849:Chromatography, Gas / Q000379:methods*; D005243:Feces / Q000737:chemistry*; D006801:Humans; D006862:Hydrogen Sulfide / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D008163:Luminescent Measurements; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013440:Sulfides; D013455:Sulfur / Q000032:analysis; D017967:Zinc Compounds
D013440:Sulfides; D017967:Zinc Compounds; D013455:Sulfur; C031238:zinc sulfide; D006862:Hydrogen Sulfide