Phosphodiesterase production in an aqueous two-phase system by Nicotiana tabacum 1507.
Studies were conducted on the production of phosphodiesterases by Nicotiana tabacum 1507 cell suspension in an aqueous two-phase system formed by adding 4% polyethylene glycol (mol wt 20,000) and 7.5% dextran (mol wt 70,000) to the medium. The time course of growth, biosynthesis, secretion, and partitioning of phosphodiesterases was followed in comparison with N. tabacum 1507 cultivation as a free suspension. Partitioning of phosphodiesterases took place mainly in the bottom dextran phase, and a possibility was revealed for obtaining an enzyme preparation with high phosphodiesterase activity.
Ilieva
M
M
Bulgarian Academy of Sciences, Institute of Microbiology, Laboratory of Biotechnology and Industrial Microbiology, Plovdiv. stoilov@plovdiv.techno-link.com
Pavlov
A
A
Bacalova
A
A
D001671:Biochemistry / Q000379:methods*; D002455:Cell Division; D006863:Hydrogen-Ion Concentration; D010727:Phosphoric Diester Hydrolases / Q000096:biosynthesis* / Q000737:chemistry; D010944:Plants / Q000378:metabolism; D010947:Plants, Toxic; D011092:Polyethylene Glycols / Q000494:pharmacology; D013997:Time Factors; D014026:Nicotiana / Q000201:enzymology*
D011092:Polyethylene Glycols; D010727:Phosphoric Diester Hydrolases