[Changes of K+ channel current in cerebellar cortical neuron of apoptosis induced with H2O2].
The neurons dissociated from the cerebellar cortex of neonatal SD rat were cultured, while the growth of non-neuron cells were suppressed by Ara-c. Apoptosis of the neurons were induced with H2O2. The changes of Ik were studied during apoptosis using the patch clamp technique in cell-attached configurations. The results are as follows: (1) the Ik amplitudes of the neurons were lower than that of normal at various corresponding clamp voltages; (2) the conductances, mean open time, open probability, long and short opening time constants were all lower, as compared with normal neurons. All these results indicate that the activities of K+ channels on cerebellar neurons decrease during apoptosis.
Chi
X X
XX
Department of Physiology, Sun Yat-Sen University of Medical Science, Guangzhou 510089.
Feng
J Q
JQ
Chen
P X
PX
D000818:Animals; D000831:Animals, Newborn; D017209:Apoptosis; D001693:Biological Transport, Active; D002478:Cells, Cultured; D002525:Cerebellar Cortex / Q000166:cytology* / Q000378:metabolism / Q000502:physiology; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D009474:Neurons / Q000166:cytology / Q000378:metabolism / Q000502:physiology; D011188:Potassium / Q000378:metabolism; D015221:Potassium Channels / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
D015221:Potassium Channels; D006861:Hydrogen Peroxide; D011188:Potassium