title: Evaluation of a commercial ELISA for diagnosis of paratuberculosis in cattle.
abstract: OBJECTIVE
  To evaluate sensitivity and specificity of a new ELISA for antibodies against Mycobacterium avium subsp paratuberculosis.
  
  
  METHODS
  Cross-sectional observational survey.
  
  
  METHODS
  Serum samples from 590 cattle that were infected with M avium subsp paratuberculosis and 723 cattle that were not infected.
  
  
  METHODS
  Serum samples were tested by use of an ELISA for antibodies against M avium subsp paratuberculosis.
  
  
  RESULTS
  Sensitivity of the test varied from 15.4 to 88.1%, depending on the clinical stage and bacterial shedding status of the cattle.
  
  
  CONCLUSIONS
  Results obtained with use of the new ELISA agreed favorably with those of a previous ELISA. Practitioners must be aware of variability in the sensitivity of the test, which depends on the clinical and shedding status of the cattle, because this may affect interpretation of test results.
authors.affiliation: USDA:APHIS Centers for Epidemiology and Animal Health, Fort Collins, CO 80521, USA.
authors.forename: D A
authors.initials: DA
authors.lastname: Dargatz
authors.forename: B A
authors.initials: BA
authors.lastname: Byrum
authors.forename: L K
authors.initials: LK
authors.lastname: Barber
authors.forename: R W
authors.initials: RW
authors.lastname: Sweeney
authors.forename: R H
authors.initials: RH
authors.lastname: Whitlock
authors.forename: W P
authors.initials: WP
authors.lastname: Shulaw
authors.forename: R H
authors.initials: RH
authors.lastname: Jacobson
authors.forename: J R
authors.initials: JR
authors.lastname: Stabel
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis*; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology; D003430:Cross-Sectional Studies; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D016927:Mycobacterium avium subsp. paratuberculosis / Q000276:immunology*; D010283:Paratuberculosis / Q000175:diagnosis* / Q000276:immunology; D012680:Sensitivity and Specificity
chemical_list: D000907:Antibodies, Bacterial