Metabolite and enzyme profiles of glycogen metabolism in Methanococcoides methylutens.
When a buffered anaerobic cell suspension of Methanococcoides methylutens was maintained under methanol-limited conditions, intracellular glycogen and hexose phosphates were consumed rapidly and a very small amount of methane formed at 4 h of a starvation period. When methanol was supplemented after a total of 20 h of starvation, a reverse pattern was observed: the glycogen level and the hexose phosphate pool increased, and formation of methane took place after a lag period of 90 min. A considerable amount of methane was formed in 120 min after its detection with a rate of 0.18 micromol mg(-1) protein min(-1). When methane formation decreased after 270 min of incubation and finally came to a halt, probably due to complete assimilation of supplemented methanol, the levels of glycogen and hexose monophosphates decreased once again. However fructose 1,6-diphosphate levels showed a continuous increase even after exhaustion of methane formation. In contrast to the hexose phosphate pool, levels of other metabolites showed a small increase after addition of methanol. The enzyme profile of glycogen metabolism showed relatively high levels of triose phosphate isomerase. Glyceraldehyde 3-phosphate dehydrogenase reacted with NADPH with a three-fold higher activity as compared to that with NADH.
Maitra
P K
PK
Agharkar Research Institute, Agarkar Road, 411 004, Pune, India. bhosale@airpune.ernet.in
Bhosale
S B
SB
Kshirsagar
D C
DC
Yeole
T Y
TY
Shanbhag
A N
AN
D000244:Adenosine Diphosphate / Q000378:metabolism; D000249:Adenosine Monophosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D002245:Carbon Dioxide / Q000378:metabolism; D003470:Culture Media; D005943:Gluconeogenesis; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism; D006003:Glycogen / Q000378:metabolism*; D006019:Glycolysis; D006600:Hexosephosphates / Q000378:metabolism; D008697:Methane / Q000378:metabolism; D000432:Methanol / Q000378:metabolism; D017019:Methanosarcinaceae / Q000201:enzymology / Q000378:metabolism*; D009243:NAD / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D014305:Triose-Phosphate Isomerase / Q000378:metabolism
D003470:Culture Media; D006600:Hexosephosphates; D009243:NAD; D002245:Carbon Dioxide; D000249:Adenosine Monophosphate; D009249:NADP; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D006003:Glycogen; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D014305:Triose-Phosphate Isomerase; D008697:Methane; D000432:Methanol