title: Mild heat shock induces proliferation, alkaline phosphatase activity, and mineralization in human bone marrow stromal cells and Mg-63 cells in vitro.
abstract: Bone formation has been shown to be stimulated by local diathermy in vivo; however, the mechanisms involved in this heat-induced osteogenesis are unclear. In this study, we investigated the direct effect of temperature on human bone marrow-derived stromal cells (BMSCs) and the human osteoblast-like, osteosarcoma-derived MG-63 cells in culture conditions. Both cell types were shown to tolerate the transient exposure to mild heat shock conditions (1 h at 39-41 degrees C), and long-term (96 h) exposure at 39 degrees C stimulated DNA synthesis in BMSC but caused growth arrest in MG-63 cells. Furthermore, 1-h exposure to higher temperatures (42.5-45 degrees C) or continuous 96-h exposure to 40 degrees C or 41 degrees C inhibited the proliferation of both BMSCs and MG63 cells. The level of alkaline phosphatase (ALP) in these cells linearly correlated with the increase in temperature, and the ALP expression, either at the basal level or in response to 1,25-dihydroxyvitamin D3 [1,25(OH)2D3], was enhanced after a single 1-h exposure to 42.5 degrees C. In addition, continuous incubation at 39 degrees C or repeated transient exposure to 39/41 degrees C greatly enhanced the ability of BMSCs to form mineralizing nodules. The heat shock protein HSP70, which was expressed constitutively by BMSCs, was found to be up-regulated by hyperthermia (39 degrees C) and down-regulated at 33 degrees C. The expression of HSP70 could be induced in MG-63 cells by both low- and high-temperature conditions. These data suggest that treatment with a mild heat shock induces the proliferation and differentiation of osteoprogenitor cells, and the direct effects of temperature on bone-forming cells might be one of the mechanisms involved in heat-induced bone formation in vivo.
authors.affiliation: Human Metabolism and Clinical Biochemistry, Division of Biochemical and Musculoskeletal Medicine, University of Sheffield Medical School, United Kingdom.
authors.forename: C
authors.initials: C
authors.lastname: Shui
authors.forename: A
authors.initials: A
authors.lastname: Scutt
mesh_terms: D000469:Alkaline Phosphatase / Q000096:biosynthesis* / Q000235:genetics; D001854:Bone Marrow Cells / Q000187:drug effects / Q000378:metabolism*; D001859:Bone Neoplasms / Q000378:metabolism / Q000473:pathology*; D002113:Calcification, Physiologic; D002117:Calcitriol / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured / Q000187:drug effects / Q000378:metabolism; D004261:DNA Replication / Q000187:drug effects; D003972:Diathermy; D004790:Enzyme Induction / Q000187:drug effects; D005786:Gene Expression Regulation; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics; D006358:Hot Temperature; D006801:Humans; D006979:Hyperthermia, Induced; D008297:Male; D008875:Middle Aged; D010006:Osteoblasts / Q000187:drug effects / Q000378:metabolism*; D012516:Osteosarcoma / Q000378:metabolism / Q000473:pathology*; D017154:Stromal Cells / Q000187:drug effects / Q000378:metabolism; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism
chemical_list: D018840:HSP70 Heat-Shock Proteins; D000469:Alkaline Phosphatase; D002117:Calcitriol