title: Introduction of single base substitutions at homologous chromosomal sequences by adeno-associated virus vectors.
abstract: Adeno-associated virus (AAV) vectors can modify homologous chromosomal sequences at high rates. This gene targeting transduction pathway is distinct from the integrating and episomal pathways used in gene addition approaches. In previous studies, AAV vectors were used to introduce small insertion and deletion mutations at homologous chromosomal loci. Here we show that AAV-mediated gene targeting can also be used to introduce all possible types of single base substitution mutations at the endogenous single-copy hypoxanthine phosphoribosyl transferase locus. Southern blot and sequence analysis showed that the point mutations were introduced with high fidelity. We also show that AAV vectors can repair chromosomal alkaline phosphatase genes containing point mutations. Our results suggest that AAV vectors can be used to introduce single base substitutions at high frequencies in normal human cells, including the correction of point mutations responsible for genetic diseases.
authors.affiliation: Division of Hematology, University of Washington, Seattle, Washington 98195, USA.
authors.forename: N
authors.initials: N
authors.lastname: Inoue
authors.forename: R
authors.initials: R
authors.lastname: Dong
authors.forename: R K
authors.initials: RK
authors.lastname: Hirata
authors.forename: D W
authors.initials: DW
authors.lastname: Russell
mesh_terms: D000469:Alkaline Phosphatase / Q000235:genetics; D015139:Blotting, Southern; D002478:Cells, Cultured; D002875:Chromosomes / Q000648:ultrastructure*; D000229:Dependovirus / Q000235:genetics*; D005347:Fibroblasts / Q000378:metabolism; D017353:Gene Deletion; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D008297:Male; D008957:Models, Genetic; D009154:Mutation; D010957:Plasmids / Q000378:metabolism; D017354:Point Mutation; D012326:RNA Splicing; D012190:Retroviridae / Q000235:genetics
chemical_list: D000469:Alkaline Phosphatase