The role of duck hepatitis B virus and aflatoxin B1 in the induction of oxidative stress in the liver.
The aim of our study was to use the Pekin duck model to investigate the interactions between hepadnaviral infection and aflatoxin B1 (AFB1) exposure including the role of both factors in the induction of oxidative stress in the liver. AFB1 exposure of duck hepatitis B virus (DHBV) infected Pekin ducks induced a significant increase in viral replication associated with an intense biliary ductular cells proliferation. Interestingly, extremely high levels of AFB1-DNA adducts (40-120 pmol AFB1-Fapy/mg DNA) and AFB1-albumin adducts (1,500-3,000 pg AFB1-lys Eq/mg albumin) were detected in duck liver and serum respectively, as compared to other animal species exposed to a similar AFB1 dose. DHBV infection was found to induce a non-significant increase in AFB1-albumin adduct levels in duck serum. During the treatment duration there was no effect on formation of oxidative base damage within DNA and no effect on oxidative lipid peroxidation following either viral infection or AFB1 exposure. In terms of hepatic antioxidant enzymes (catalase, superoxide dismutase (SOD), glutathione peroxidase) a significant increase in SOD activity occurred following AFB1 exposure, but not DHBV infection, but this was observed only after the cessation of treatment, when biliary ductular cells proliferation was reduced.
Barraud
L
L
INSERM U271, Grenoble, France.
Douki
T
T
Guerret
S
S
Chevallier
M
M
Jamard
C
C
Trepo
C
C
Wild
C P
CP
Cadet
J
J
Cova
L
L
D000080242:8-Hydroxy-2'-Deoxyguanosine; D016604:Aflatoxin B1 / Q000633:toxicity*; D000818:Animals; D002374:Catalase / Q000378:metabolism; D002417:Cattle; D004247:DNA / Q000378:metabolism; D018736:DNA Adducts / Q000378:metabolism; D004279:DNA, Viral / Q000097:blood / Q000378:metabolism; D003841:Deoxycytidine / Q000031:analogs & derivatives / Q000378:metabolism; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000378:metabolism; D004372:Ducks; D005979:Glutathione Peroxidase / Q000378:metabolism; D018347:Hepadnaviridae Infections / Q000378:metabolism* / Q000821:virology; D015611:Hepatitis B Virus, Duck / Q000502:physiology*; D006524:Hepatitis, Viral, Animal / Q000378:metabolism* / Q000821:virology; D015227:Lipid Peroxidation; D008099:Liver / Q000187:drug effects*; D018384:Oxidative Stress; D012710:Serum Albumin, Bovine / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism
D018736:DNA Adducts; D004279:DNA, Viral; D003841:Deoxycytidine; D012710:Serum Albumin, Bovine; C074071:5-hydroxy-2'-deoxycytidine; D000080242:8-Hydroxy-2'-Deoxyguanosine; D004247:DNA; D016604:Aflatoxin B1; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D003849:Deoxyguanosine