Developmental regulation of transcription by a tissue-specific TAF homolog.
Alternate forms of the general transcription machinery have been described in several tissues or cell types. However, the role of tissue-specific TBP-associated factors (TAF(II)s) and other tissue-specific transcription components in regulating differential gene expression during development was not clear. Here we show that the cannonball gene of Drosophila encodes a cell type-specific homolog of a more ubiquitously expressed component of the general transcription factor TFIID. cannonball is required in vivo for high level transcription of a set of stage- and tissue-specific target genes during male gametogenesis. Regulation of transcription by cannonball is absolutely required for spermatogenesis, as null mutations block meiotic cell cycle progression and result in a complete failure of spermatid differentiation. Our results demonstrate that cell type-specific TAF(II)s play an important role in developmental regulation of gene expression.
Hiller
M A
MA
Department of Developmental Biology, Stanford University School of Medicine, Stanford, California 94305-5329, USA.
Lin
T Y
TY
Wood
C
C
Fuller
M T
MT
D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D002453:Cell Cycle / Q000235:genetics; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism* / Q000502:physiology; D004330:Drosophila / Q000235:genetics; D029721:Drosophila Proteins; D018507:Gene Expression Regulation, Developmental; D017930:Genes, Reporter; D008297:Male; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009154:Mutation; D020033:Protein Isoforms; D012191:Retroviridae Proteins / Q000737:chemistry / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D013091:Spermatogenesis / Q000235:genetics / Q000502:physiology; D015534:Trans-Activators / Q000737:chemistry / Q000378:metabolism*; D035362:Transcription Factor TFIID; D014157:Transcription Factors / Q000235:genetics* / Q000502:physiology; D019473:Transcription Factors, TFII / Q000235:genetics; D014158:Transcription, Genetic
D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D020033:Protein Isoforms; D012191:Retroviridae Proteins; D015534:Trans-Activators; D035362:Transcription Factor TFIID; D014157:Transcription Factors; D019473:Transcription Factors, TFII; C074061:bel1 protein, Human foamy virus; C426105:can protein, Drosophila