[Expression of surfactant protein SP-A, SP-B, and SP-C mRNA in lungs of rats with bleomycin-induced pulmonary fibrosis].
OBJECTIVE
The expressions of surfactant protein (SP)SP-A, SP-B, and SP-CmRNA in lungs of rats with bleomycin-induced pulmonary fibrosis were studied.


METHODS
A single dose of bleomycin(BLM) was intratracheal injected to induce pulmonary fibrosis of rats. Animals were killed at day 3, 7, 14 and 28 after BLM administration. The total RNA was extracted from the lung tissue. The expressions of SPsmRNA were analyzed with Northern blot.


RESULTS
The number of alveolar type II epithelial cells increased in BLM-administered rats. The expressions of SP-A, SP-B and SP-CmRNA decreased at day 3 after BLM administration and decreased maximally at day 7, and then began to increase at day 14 and significantly increased at day 28, though they were still below the control levels.


CONCLUSIONS
The results show that the changes of expressions of SP-A, SP-B and SP-CmRNA occur during the development of bleomycin-induced pulmonary fibrosis in rats and they may play a role in the pathogenesis of lung fibrosis.
Song
M
M
First Hospital of Beijing Medical University Beijing 100034.
He
B
B
Qiu
Z
Z
D000818:Animals; D001761:Bleomycin; D008297:Male; D011510:Proteolipids / Q000096:biosynthesis* / Q000235:genetics; D011658:Pulmonary Fibrosis / Q000139:chemically induced / Q000378:metabolism*; D037662:Pulmonary Surfactant-Associated Protein A; D037661:Pulmonary Surfactant-Associated Proteins; D011663:Pulmonary Surfactants / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
D011510:Proteolipids; D037662:Pulmonary Surfactant-Associated Protein A; D037661:Pulmonary Surfactant-Associated Proteins; D011663:Pulmonary Surfactants; D012333:RNA, Messenger; D001761:Bleomycin