title: Intracameral and Lenticular Penetration of Locally Applied Deuterium-Labeled Vitamin E in Rats.
abstract: Purpose: To confirm the intraocular dynamics of 1% deuterium (D) labeled alpha-tocopheral acetate (VEA) solution.Methods: The concentrations of D(3)-VEA and D(3)-alpha-tocopherol (VE) derived from D(3)-VEA in the aqueous humor and lens were measured after instilling 1% D(3)-VEA continuously into the cul-de-sac of rat eyes for one and three weeks. D(3)-VEA and D(3)-VE concentrations were determined by gas chromatograph/mass spectrometry.Results: D(3)-VEA and D(3)-VE concentrations in the aqueous humor after one and three weeks of continuous administration were 93.1 and 498.9, and 9.4 and 21.5 ng/mL, respectively. The concentrations in the lens were 15.0 and 6.1, and 9.8 and 4.8 ng/g, respectively.Conclusion: The penetration of VEA into the aqueous humor and lens by eyedrop application was confirmed.
authors.affiliation: Department of Ophthalmology, Kanazawa Medical University, Takayan, Japan
authors.forename: M
authors.initials: M
authors.lastname: Nagata
authors.forename: K
authors.initials: K
authors.lastname: Kawazu
authors.forename: Y
authors.initials: Y
authors.lastname: Midori
authors.forename: M
authors.initials: M
authors.lastname: Kojima
authors.forename: E
authors.initials: E
authors.lastname: Shirasawa
authors.forename: K
authors.initials: K
authors.lastname: Sasaki