Links between the immune and coagulation systems: how do "antiphospholipid antibodies" cause thrombosis?
Inflammation and immune activation have been associated with thrombosis in a number of settings. We have been interested in the question of how the presence of a type of autoantibody, so-called "antiphospholipid" antibody, leads to thrombosis. Several mechanisms have been proposed including modulation of tissue factor expression, enhancement of procoagulant binding to platelets, and interference with antithrombotic mechanisms. We developed a cell-based model of coagulation that, unlike current coagulation assays, reflects some of the in vivo activities of "antiphospholipid" antibodies. "Antiphospholipid" antibodies against the phospholipid-binding protein beta-2-glycoprotein-1 enhance thrombin generation in this model system, primarily by enhancing procoagulant reactions on tissue factor-bearing cells.
Hoffman
M
M
Duke University Medical Center, Durham, NC, USA. maureane@med.unc.edu
Monroe
D M
DM
Roubey
R A
RA
D017152:Antibodies, Antiphospholipid / Q000276:immunology*; D001777:Blood Coagulation; D006801:Humans; D009000:Monocytes / Q000276:immunology; D013917:Thrombin / Q000096:biosynthesis / Q000276:immunology; D013927:Thrombosis / Q000209:etiology* / Q000276:immunology; D001620:beta-Thromboglobulin / Q000276:immunology
D017152:Antibodies, Antiphospholipid; D001620:beta-Thromboglobulin; D013917:Thrombin