IL-10 augments the IFN-gamma and TNF-alpha induced TARC production in HaCaT cells: a possible mechanism in the inflammatory reaction of atopic dermatitis.
The CC-chemokine TARC is known to be a ligand for the CCR4 receptor which in turn is known to be expressed selectively on the Th(2)-subset of lymphocytes. Atopic dermatitis is generally believed to be a Th(2)-type disease, and TARC has been shown to be expressed in the skin lesions of a murine model of AD. IL-10 is an interleukine generally known for its ability to inhibit cytokine production, however it has been found to be highly expressed in the skin from AD patients. We show in this report that IL-10 is able to augment the TARC inducing effects of TNFalpha and IFNgamma in HaCaT cells, a property that may be important in the determination of the composition of the cells of the inflammation in the skin of AD patients. In addition, we show that the IL10 agonist IT 9302, a nona-peptide from the carboxylic end of IL-10, has the same effect on TARC production from HaCaT cells.
Vestergaard
C
C
Department of Dermatology, Marselisborg Hospital, University of Aarhus, Research Laboratory B, Oerumsgade 11, 8000 Aarhus C, Aarhus, Denmark. chr-vest@post9.tele.dk
Kirstejn
N
N
Gesser
B
B
Mortensen
J T
JT
Matsushima
K
K
Larsen
C G
CG
D002461:Cell Line, Transformed; D054414:Chemokine CCL17; D019742:Chemokines, CC / Q000276:immunology*; D003876:Dermatitis, Atopic / Q000209:etiology / Q000276:immunology; D004347:Drug Interactions; D006801:Humans; D007249:Inflammation; D007371:Interferon-gamma / Q000276:immunology* / Q000494:pharmacology; D016753:Interleukin-10 / Q000276:immunology* / Q000494:pharmacology; D015603:Keratinocytes / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology* / Q000494:pharmacology
C106230:CCL17 protein, human; D054414:Chemokine CCL17; D019742:Chemokines, CC; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D007371:Interferon-gamma