[Studies on lipase production from Candida rugosa].
We studied some factors affecting the lipase production from candida rugosa, they mainly included medium compositions and culture condition. The result showed that the optimal medium compositions for lipase production are 0.1% glucose 4.0% olive oil (carbon source), 0.3% NH4NO3(nitrogen source), 1.2% K2HPO4 and 0.4% MgSO4.7H2O. And the optimal culture condition is initial pH6.5, temperature 30 degrees C, agitation 180 r/min and time 60 h. As a result, and the lipase activity could reach 19.5 u/mL. Meanwhile we found that the surfactant could be helpful to the lipase production, and the optimal surfactant concentration was 0.03% GPE. The lipase activity was improved by more than 170% after we optimized the medium compositions and culture condition. While in a 5L fermentator, the lipase activity of fermentation broth could reach 33.5 u/mL within 48 hours.
Song
Q X
QX
State Key Laboratory of Bioreactor Engineering, Research Institute of Biochemistry, East China University of Science & Technology, Shanghai 200237, China.
Lin
J P
JP
Rong
Y P
YP
Wei
D Z
DZ
D002175:Candida / Q000201:enzymology*; D003470:Culture Media; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D008049:Lipase / Q000096:biosynthesis*; D013696:Temperature
D003470:Culture Media; D008049:Lipase