Mitochondrial membrane potential in density-separated trout erythrocytes exposed to oxidative stress in vitro.
Previous literature reports have demonstrated that nucleated trout erythrocytes in condition of oxidative stress are subjected to DNA and membrane damage, and inactivation of glutathione peroxidase. The present study was undertaken to investigate if mitochondrial membrane potential in stressed conditions was also influenced. Density-separated trout erythrocyte fractions, obtained using a discontinuous Percoll gradient, were submitted to stress conditions and the mitochondrial membrane potential was determined by means of cytofluorimetric analysis after incubation of each subfraction with JC-1, a mitochondrial specific fluorescent probe. The results clearly show that the mitochondrial membrane potential decreased significantly in all erythrocyte fractions, also if the oxidative effect on mitochondria is more severe with increased density (age) of the cell. Ebselen was very effective in preventing mitochondrial depolarization in young as well as in old erythrocytes.
Tiano
L
L
Department of Biology MCA, University of Camerino, MC, Italy. l.tiano@cambio.unicam.it
Fedeli
D
D
Ballarini
P
P
Santoni
G
G
Falcioni
G
G
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001393:Azoles / Q000494:pharmacology; D001562:Benzimidazoles; D002232:Carbocyanines; D016922:Cellular Senescence; D002839:Chromans / Q000494:pharmacology; D003497:Cyclic N-Oxides / Q000494:pharmacology; D004912:Erythrocytes / Q000737:chemistry*; D005434:Flow Cytometry; D005456:Fluorescent Dyes; D005609:Free Radicals / Q000032:analysis; D066298:In Vitro Techniques; D007425:Intracellular Membranes / Q000737:chemistry*; D054833:Isoindoles; D008564:Membrane Potentials; D018613:Microscopy, Confocal; D008928:Mitochondria / Q000737:chemistry*; D015394:Molecular Structure; D016566:Organoselenium Compounds / Q000494:pharmacology; D018384:Oxidative Stress / Q000187:drug effects; D017382:Reactive Oxygen Species; D014337:Trout
D000975:Antioxidants; D001393:Azoles; D001562:Benzimidazoles; D002232:Carbocyanines; D002839:Chromans; D003497:Cyclic N-Oxides; D005456:Fluorescent Dyes; D005609:Free Radicals; D054833:Isoindoles; D016566:Organoselenium Compounds; D017382:Reactive Oxygen Species; C068624:5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolocarbocyanine; C042986:ebselen; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid; C003959:TEMPO