Screening of Bcr-Abl transcripts in Philadelphia negative essential thrombocythemia.
Essential thrombocythemia (ET) is a chronic myeloproliferative disorder characterised by the absence of the Philadelphia (Ph+) chromosome. Recent studies have reported controversial results relating to BCR-ABL rearrangements in ET patients. We studied 44 Ph-negative ET patients with the RT-PCR technique at diagnosis or during the follow-up. None of them showed any of the BCR-ABL transcript actually described by others in ET; neither the "classical" P210 nor the P190 or P230 variants. Our results confirm the absence of BCR-ABL abnormalities in Ph-negative ET patients.
Tiribelli
M
M
Chair and Division of Hematology, Department of Medical and Morphological Research, University of Udine, Italy.
Michelutti
A
A
Damante
G
G
Pellizzari
L
L
Martinelli
G
G
Amabile
M
M
Russo
D
D
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D016044:Fusion Proteins, bcr-abl / Q000235:genetics*; D005820:Genetic Testing; D014644:Genetic Variation / Q000235:genetics; D006801:Humans; D008297:Male; D008875:Middle Aged; D010677:Philadelphia Chromosome; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013920:Thrombocythemia, Essential / Q000175:diagnosis / Q000235:genetics*
D012333:RNA, Messenger; D016044:Fusion Proteins, bcr-abl