Mitomycin c influence on inhibition of cellular proliferation and subsequent synthesis of type I collagen and laminin in primary and recurrent pterygia.
The purpose was to investigate the biological effects of mitomycin C (MMC) on the inhibition of cellular proliferation and the subsequent synthesis of extracellular matrix like type I collagen (PIP) and laminin on primary and recurrent pterygia. The 3H-thymidine assay for cellular proliferation was consistent with the MMC dose and time response of both pterygia. The cellular inhibitory effect of MMC was greater on recurrent pterygial cells than primary ones. The inhibition of laminin by MMC followed the dose and time-response curves, with a stronger effect found in recurrent pterygial cells, but no significant difference between the two types concerning type I collagen synthesis. The inhibition of fibroblast proliferation and subsequent synthesis of laminin by MMC was more marked in recurrent pterygium than the primary one.
Lee
J S
JS
Department of Ophthalmology, Research Institute of Medical Science, College of Medicine, Pusan National University, Pusan City, Korea.
Oum
B S
BS
Lee
S H
SH
D000328:Adult; D002455:Cell Division / Q000187:drug effects; D003094:Collagen / Q000096:biosynthesis*; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D005109:Extracellular Matrix / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D007797:Laminin / Q000096:biosynthesis*; D008297:Male; D016685:Mitomycin / Q000494:pharmacology*; D011625:Pterygium / Q000378:metabolism*; D012008:Recurrence; D013997:Time Factors
D007797:Laminin; D016685:Mitomycin; D003094:Collagen