[Human angiogenin: expression, purification, biological assay].
Angiogenin cDNA was obtained by RT-PCR, and cloned into the fusion expression vector pRSETB. The recombinant Angiogenin protein was fused with His6 at its N-terminal and expressed as inclusion body. The expression level was about 10% of the total bacteria protein. After dissolved in 8 mol/L urea, the recombinant protein was purified by Ni2(+)-NTA chelating resin, according to the high affinity of His6 with Ni2+. The biological assay indicated that purified rhANG could induced the new blood vessel formation of CAM and degraded tRNA in vitro.
Yang
H
H
Fourth Military Medical University Biotechnology Center, Xi'an, China. bio707@fmmu.edu.cn
Zhang
Y Q
YQ
Yan
Z
Z
Han
W
W
Yao
L B
LB
Su
C Z
CZ
D043925:Angiogenesis Inducing Agents / Q000096:biosynthesis*; D000818:Animals; D002642:Chick Embryo; D002846:Chromatography, Affinity; D006801:Humans; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis*; D012259:Ribonuclease, Pancreatic / Q000096:biosynthesis* / Q000302:isolation & purification / Q000494:pharmacology
D043925:Angiogenesis Inducing Agents; D011993:Recombinant Fusion Proteins; C046366:angiogenin; D012259:Ribonuclease, Pancreatic