title: Tissue-specific in vivo inhibition of DNase I gene expression by somatostatin.
abstract: Administration of somatostatin to rats induced a transient reduction of serum levels of deoxyribonuclease I (DNase I) activity in a dose-dependent manner, followed by a substantial decrease of DNase I activity in the lower gut. Activity in the parotid gland, liver, and kidney did not change. Real-time PCR analysis of the DNase I gene transcript in ileum indicated that the decrease was due to down-regulation of gene expression. Based on these responses, rat tissues expressing DNase I could be classified into two types, somatostatin-sensitive and somatostatin-resistant, and the level of DNase I activity in the lower gut seems to be controlled by somatostatin.
authors.affiliation: Department of Biology, Fukui Medical University, Matsuoka, Fukui, 910-1193, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Yasuda
authors.forename: H
authors.initials: H
authors.lastname: Takeshita
authors.forename: M
authors.initials: M
authors.lastname: Ueki
authors.forename: R
authors.initials: R
authors.lastname: Iida
authors.forename: T
authors.initials: T
authors.lastname: Nakajima
authors.forename: S
authors.initials: S
authors.lastname: Mori
authors.forename: K
authors.initials: K
authors.lastname: Mogi
authors.forename: Y
authors.initials: Y
authors.lastname: Kaneko
authors.forename: K
authors.initials: K
authors.lastname: Kishi
mesh_terms: D000818:Animals; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D003850:Deoxyribonuclease I / Q000097:blood / Q000235:genetics* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004351:Drug Resistance; D005260:Female; D015870:Gene Expression / Q000187:drug effects*; D007082:Ileum / Q000187:drug effects / Q000201:enzymology; D008297:Male; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013004:Somatostatin / Q000008:administration & dosage / Q000494:pharmacology*; D014018:Tissue Distribution
chemical_list: D017931:DNA Primers; D012333:RNA, Messenger; D013004:Somatostatin; D003850:Deoxyribonuclease I