title: Heteronuclear 2D-correlations in a uniformly [13C, 15N] labeled membrane-protein complex at ultra-high magnetic fields.
abstract: One- and two-dimensional solid-state NMR experiments on a uniformly labeled intrinsic membrane-protein complex at ultra-high magnetic fields are presented. Two-dimensional backbone and side-chain correlations for a [U-13C, 15N] labeled version of the LH2 light-harvesting complex indicate significant resolution at low temperatures and under Magic Angle Spinning. Tentative assignments of some of the observed correlations are presented and attributed to the alpha-helical segments of the protein, mostly found in the membrane interior.
authors.affiliation: Gorlaeus Laboratories, Leiden University, The Netherlands.
authors.forename: T A
authors.initials: TA
authors.lastname: Egorova-Zachernyuk
authors.forename: J
authors.initials: J
authors.lastname: Hollander
authors.forename: N
authors.initials: N
authors.lastname: Fraser
authors.forename: P
authors.initials: P
authors.lastname: Gast
authors.forename: A J
authors.initials: AJ
authors.lastname: Hoff
authors.forename: R
authors.initials: R
authors.lastname: Cogdell
authors.forename: H J
authors.initials: HJ
authors.lastname: de Groot
authors.forename: M
authors.initials: M
authors.lastname: Baldus
mesh_terms: D000595:Amino Acid Sequence; D002247:Carbon Isotopes; D003080:Cold Temperature; D008565:Membrane Proteins / Q000737:chemistry*; D008969:Molecular Sequence Data; D009587:Nitrogen Isotopes; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry*; D012680:Sensitivity and Specificity
chemical_list: D002247:Carbon Isotopes; D008565:Membrane Proteins; D009587:Nitrogen Isotopes; D045322:Photosynthetic Reaction Center Complex Proteins