title: Rho-kinase--mediated contraction of isolated stress fibers.
abstract: It is widely accepted that actin filaments and the conventional double-headed myosin interact to generate force for many types of nonmuscle cell motility, and that this interaction occurs when the myosin regulatory light chain (MLC) is phosphorylated by MLC kinase (MLCK) together with calmodulin and Ca(2+). However, recent studies indicate that Rho-kinase is also involved in regulating the smooth muscle and nonmuscle cell contractility. We have recently isolated reactivatable stress fibers from cultured cells and established them as a model system for actomyosin-based contraction in nonmuscle cells. Here, using isolated stress fibers, we show that Rho-kinase mediates MLC phosphorylation and their contraction in the absence of Ca(2+). More rapid and extensive stress fiber contraction was induced by MLCK than was by Rho-kinase. When the activity of Rho-kinase but not MLCK was inhibited, cells not only lost their stress fibers and focal adhesions but also appeared to lose cytoplasmic tension. Our study suggests that actomyosin-based nonmuscle contractility is regulated by two kinase systems: the Ca(2+)-dependent MLCK and the Rho-kinase systems. We propose that Ca(2+) is used to generate rapid contraction, whereas Rho-kinase plays a major role in maintaining sustained contraction in cells.
authors.affiliation: Department of Structural Analysis, National Cardiovascular Center Research Institute, Suita, Osaka 565-8565, Japan. katoichi@ri.ncuc.go.jp
authors.forename: K
authors.initials: K
authors.lastname: Katoh
authors.forename: Y
authors.initials: Y
authors.lastname: Kano
authors.forename: M
authors.initials: M
authors.lastname: Amano
authors.forename: H
authors.initials: H
authors.lastname: Onishi
authors.forename: K
authors.initials: K
authors.lastname: Kaibuchi
authors.forename: K
authors.initials: K
authors.lastname: Fujiwara
mesh_terms: D002118:Calcium / Q000378:metabolism; D002458:Cell Fractionation / Q000379:methods; D002474:Cell-Free System; D003285:Contractile Proteins / Q000378:metabolism*; D005347:Fibroblasts / Q000166:cytology; D005990:Glycerol / Q000494:pharmacology; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D008297:Male; D008954:Models, Biological; D009068:Movement / Q000502:physiology*; D018994:Myosin Light Chains / Q000378:metabolism; D009219:Myosin-Light-Chain Kinase / Q000378:metabolism; D017830:Octoxynol / Q000494:pharmacology; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D022502:Stress Fibers / Q000187:drug effects / Q000502:physiology*; D020741:rho GTP-Binding Proteins / Q000378:metabolism*; D054460:rho-Associated Kinases
chemical_list: D003285:Contractile Proteins; D047908:Intracellular Signaling Peptides and Proteins; D018994:Myosin Light Chains; D017830:Octoxynol; D017346:Protein Serine-Threonine Kinases; D054460:rho-Associated Kinases; D009219:Myosin-Light-Chain Kinase; D020741:rho GTP-Binding Proteins; D005990:Glycerol; D002118:Calcium