title: Green marker for colonies of Bacillus subtilis.
abstract: A Bacillus subtilis plasmid encoding a green fluorescence protein gene (gfp) was constructed. The fluorescence of B. subtilis colonies having this plasmid on agar plates was so high that they could be readily discerned visually under UV light. The fluorescence could be effectively expressed in three ways (i) through use of a strong bsr promoter (blasticidin S resistance gene), (ii) by efficient translation with the bsr translation system, and (iii) through increase in the copy number per cell. The high stability of the GFP plasmid was demonstrated by using more complicated growth conditions without any antibiotic for selection.
authors.affiliation: Mitsubishi Kasei Institute of Life Sciences, Machida-shi, Tokyo, Japan. ita@libra.ls.m-kagaku.co.jp
authors.forename: M
authors.initials: M
authors.lastname: Itaya
authors.forename: S M
authors.initials: SM
authors.lastname: Shaheduzzaman
authors.forename: K
authors.initials: K
authors.lastname: Matsui
authors.forename: A
authors.initials: A
authors.lastname: Omori
authors.forename: T
authors.initials: T
authors.lastname: Tsuji
mesh_terms: D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000235:genetics / Q000254:growth & development*; D001483:Base Sequence; D015415:Biomarkers; D004269:DNA, Bacterial; D005453:Fluorescence; D018628:Gene Dosage; D005798:Genes, Bacterial; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D010957:Plasmids
chemical_list: D015415:Biomarkers; D004269:DNA, Bacterial; D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins