Isolation and characterization of velutin, a novel low-molecular-weight ribosome-inactivating protein from winter mushroom (Flammulina velutipes) fruiting bodies.
From the fruiting bodies of the edible mushroom Flammulina velutipes a single-chained ribosome inactivating protein with a molecular weight of 13.8 kDa was isolated with a procedure involving ion exchange chromatography on DEAE-cellulose and SP-Sepharose and affinity chromatography on Affi-gel blue gel. The protein was novel in that it possessed a molecular weight lower than those of previously reported RIPs and that it was capable of inhibiting human immunodeficiency virus (HIV-1) reverse transcriptase, beta-glucosidase and beta-glucuronidase. Its N-terminal sequence exhibited a certain degree of similarity to those of plant ribosome inactivating proteins.
Wang
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Department of Microbiology, China Agricultural University, Beijing.
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D000363:Agaricales / Q000737:chemistry*; D000595:Amino Acid Sequence; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D005656:Fungal Proteins / Q000737:chemistry / Q000302:isolation & purification*; D005966:Glucuronidase / Q000037:antagonists & inhibitors; D054303:HIV Reverse Transcriptase / Q000037:antagonists & inhibitors; D015497:HIV-1 / Q000187:drug effects / Q000201:enzymology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009699:N-Glycosyl Hydrolases / Q000037:antagonists & inhibitors*; D011500:Protein Synthesis Inhibitors; D018894:Reverse Transcriptase Inhibitors / Q000378:metabolism; D054788:Ribosome Inactivating Proteins; D012270:Ribosomes / Q000187:drug effects*; D001617:beta-Glucosidase / Q000037:antagonists & inhibitors
D004791:Enzyme Inhibitors; D005656:Fungal Proteins; D005966:Glucuronidase; D054303:HIV Reverse Transcriptase; D009699:N-Glycosyl Hydrolases; D011500:Protein Synthesis Inhibitors; D018894:Reverse Transcriptase Inhibitors; D054788:Ribosome Inactivating Proteins; D001617:beta-Glucosidase; C425465:Velutin protein, Flammulina velutipes