[Modulation of endothelin-1 on pulmonary surfactant secretion].
The effects of endothelin-1 (ET-1) at physiological level on the secretion of pulmonary surfactant (PS) were studied on isolated nonperfused rat lungs. The results showed that ET-1 (10(-12) and 10(-10) mol/L) increased the basal secretion of total phospholipids (TP) and phosphatidylcholine (PC), and potentiated the stimulatory effect of intermittent lung inflation on the secretion of TP and PC. The effect of ET-1 could be inhibited by H7, a protein kinase C (PKC) inhibitor. When the cultured lung tissue was exposed respectively to ET-1 (10(-12) and 10(-10) mol/L) for 1 h and 4 h, the content of ET in the lung tissue increased significantly. These results suggest that ET-1 at the physiological concentration is involved in modulating PS secretion through a PKC-dependent pathway, and can also promote ET production in lungs.
Luo
Z Q
ZQ
Department of Physiology, Hunan Medical University, Changsha 410078.
Sun
X H
XH
D000818:Animals; D046508:Culture Techniques; D019332:Endothelin-1 / Q000494:pharmacology*; D005260:Female; D008168:Lung / Q000378:metabolism*; D008297:Male; D010713:Phosphatidylcholines / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism; D011663:Pulmonary Surfactants / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D019332:Endothelin-1; D010713:Phosphatidylcholines; D010743:Phospholipids; D011663:Pulmonary Surfactants