Anabaena apoflavodoxin hydrogen exchange: on the stable exchange core of the alpha/beta(21345) flavodoxin-like family.
An important issue in modern protein biophysics is whether structurally homologous proteins share common stability and/or folding features. Flavodoxin is an archetypal alpha/beta protein organized in three layers: a central beta-sheet (strand order 21345) flanked by helices 1 and 5 on one side and helices 2, 3, and 4 on the opposite side. The backbone internal dynamics of the apoflavodoxin from Anabaena is analyzed here by the hydrogen exchange method. The hydrogen exchange rates indicate that 46 amide protons, distributed throughout the structure of apoflavodoxin, exchange relatively slowly at pH 7.0 (k(ex) < 10(-1) min(-1)). According to their distribution in the structure, protein stability is highest on the beta-sheet, helix 4, and on the layer formed by helices 1 and 5. The exchange kinetics of Anabaena apoflavodoxin was compared with those of the apoflavodoxin from Azotobacter, with which it shares a 48% sequence identity, and with Che Y and cutinase, two other alpha/beta (21345) proteins with no significant sequence homology with flavodoxins. Both similarities and differences are observed in the cores of these proteins. It is of interest that a cluster of a few structurally equivalent residues in the central beta-strands and in helix 5 is common to the cores.
Langdon
G M
GM
Instituto de Estructura de la Materia, C.S.I.C., Madrid, Spain.
Jiménez
M A
MA
Genzor
C G
CG
Maldonado
S
S
Sancho
J
J
Rico
M
M
D000595:Amino Acid Sequence; D017033:Anabaena / Q000737:chemistry*; D001059:Apoproteins / Q000737:chemistry*; D016384:Consensus Sequence; D000458:Cyanobacteria; D003903:Deuterium / Q000737:chemistry*; D005418:Flavodoxin / Q000737:chemistry*; D006859:Hydrogen / Q000737:chemistry* / Q000378:metabolism*; D006860:Hydrogen Bonding; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008958:Models, Molecular; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D011489:Protein Denaturation; D017510:Protein Folding; D017433:Protein Structure, Secondary; D011506:Proteins / Q000378:metabolism*; D011868:Radioisotopes; D016415:Sequence Alignment
D001059:Apoproteins; D005418:Flavodoxin; D011506:Proteins; D011868:Radioisotopes; C026932:apoflavodoxin; D006859:Hydrogen; D003903:Deuterium