Measurement of nicotine in hair by reversed-phase high-performance liquid chromatography with electrochemical detection.
We have developed an assay for nicotine in hair based on reversed-phase HPLC with electrochemical detection. The method uses a low-metal, high-purity silica reversed-phase column. We have investigated the washing, digestion and extraction procedures and discuss the important points in the HPLC method development. The assay is presented as an application in a population of exposed and non-exposed children. Analytical parameters are satisfactory with linearity, recoveries, limit of quantitation and precision all suitable for epidemiological studies involving environmental tobacco smoke exposure assessment.
Mahoney
G N
GN
Laboratory Services, Capital Coast Health Limited, Wellington, New Zealand. graeme.mahoney@wnhealth.co.nz
Al-Delaimy
W
W
D002675:Child, Preschool; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004563:Electrochemistry / Q000379:methods*; D004781:Environmental Exposure; D006197:Hair / Q000737:chemistry*; D006801:Humans; D007223:Infant; D009538:Nicotine / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014028:Tobacco Smoke Pollution
D014028:Tobacco Smoke Pollution; D009538:Nicotine