Influence of methylfenpropidine on growth, sterol content and fatty acid composition of Candida albicans.
The effect of methylfenpropidine on growth, lipid contents, sterol and fatty acid composition was investigated in 5 strains of Candida albicans. The sensitivity of the strains decreased in the order: wild strains > erg+ ade nysR > ade nysR erg (defective delta (8-7)-isomerase) > ade nysR erg (defective delta 5-desaturase). The presence of the inhibitor influenced fecosterol isomerization, episterol dehydrogenation, zymosterol transmethylation, ignosterol reduction and squalene epoxidation. Methylfenpropidine also induced changes in fatty acid composition, causing a reduction of the palmitic and oleic acid content with a concomitant elevation of stearic, linoleic and linolenic acid levels. The lipid unsaturation index slightly increased. Morphological changes of wild strains were observed after the fungicide treatment.
Sajbidor
J
J
Department of Biochemical Technology, Slovak University of Technology, 812 37 Bratislava. sajbidor@cvt.stuba.sk
Breierová
E
E
Lamacka
M
M
Bohov
P
P
D002176:Candida albicans / Q000737:chemistry / Q000187:drug effects* / Q000254:growth & development; D004305:Dose-Response Relationship, Drug; D004875:Ergosterol / Q000096:biosynthesis / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D005659:Fungicides, Industrial / Q000737:chemistry / Q000494:pharmacology*; D008956:Models, Chemical; D015394:Molecular Structure; D009154:Mutation; D010880:Piperidines / Q000494:pharmacology*; D013261:Sterols / Q000737:chemistry
D005227:Fatty Acids; D005659:Fungicides, Industrial; D010880:Piperidines; D013261:Sterols; C434366:methylfenpropidine; D004875:Ergosterol