title: Modulation of intestinal and liver fatty acid-binding proteins in Caco-2 cells by lipids, hormones and cytokines.
abstract: Intestinal and liver fatty acid binding proteins (I- and L-FABP) are thought to play a role in enterocyte fatty acid (FA) trafficking. Their modulation by cell differentiation and various potential effectors was investigated in the human Caco-2 cell line. With the acquisition of enterocytic features, Caco-2 cells seeded on plastic progressively increased L-FABP quantities, whereas I-FABP was not detectable even very late in the maturation process. On permeable filters that improved differentiation markers (sucrase, alkaline phosphatase, transepithelial resistance), Caco-2 cells furthered their L-FABP content and expressed I-FABP. Western blot analysis showed a significant increase in I- and L-FABP expression following an 8-hour incubation period with butyric acid, oleic acid, and phosphatidylcholine. However, in all cases, I-FABP levels were higher than L-FABP concentrations regardless of the lipid substrates added. Similarly, hydrocortisone and insulin enhanced the cellular content of I- and L-FABP whereas leptin triggered I-FABP expression only after an 8-hour incubation. Finally, tumor necrosis factor-alpha was more effective in increasing the cytosolic amount of I-FABP levels. In conclusion, our data demonstrate that I-FABP expression is limited to fully differentiated Caco-2 cells and can be more easily regulated than L-FABP by lipids, hormones, and cytokines.
authors.affiliation: Centre de Recherche, Hôpital Sainte-Justine and Department of Nutrition, Université de Montréal, Montréal, Quebec, Canada H3T 1C5.
authors.forename: N
authors.initials: N
authors.lastname: Dubé
authors.forename: E
authors.initials: E
authors.lastname: Delvin
authors.forename: W
authors.initials: W
authors.lastname: Yotov
authors.forename: C
authors.initials: C
authors.lastname: Garofalo
authors.forename: M
authors.initials: M
authors.lastname: Bendayan
authors.forename: J H
authors.initials: JH
authors.lastname: Veerkamp
authors.forename: E
authors.initials: E
authors.lastname: Levy
mesh_terms: D018938:Caco-2 Cells / Q000166:cytology / Q000378:metabolism; D002352:Carrier Proteins / Q000378:metabolism*; D002454:Cell Differentiation / Q000502:physiology; D016207:Cytokines / Q000378:metabolism*; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids / Q000378:metabolism*; D006728:Hormones / Q000378:metabolism*; D006801:Humans; D020738:Leptin / Q000378:metabolism; D009363:Neoplasm Proteins; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D025521:Tumor Suppressor Proteins
chemical_list: D002352:Carrier Proteins; D016207:Cytokines; C493319:FABP1 protein, human; C107608:FABP7 protein, human; C493320:Fabp1 protein, mouse; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids; D006728:Hormones; D020738:Leptin; D009363:Neoplasm Proteins; D014409:Tumor Necrosis Factor-alpha; D025521:Tumor Suppressor Proteins