Identification and characterization of a novel tospovirus species using a new RT-PCR approach.
A novel tospovirus serologically distinct from all established tospovirus species was found in Thailand in Physalis minima L. The S RNA of this virus was cloned by a new RT-PCR approach revealing a nucleotide sequence of 3257 nucleotides. The ambisense RNA segment encoded a nonstructural protein (NSs) of 469 amino acids, with a predicted Mr of 53.2 kDa, and a nucleoprotein (N) of 279 amino acids and a Mr of 31.0 kDa, so far the largest N protein known for any tospovirus species. N protein sequence comparisons revealed closet relationship to the species Watermelon bud necrosis virus (58% identity), Watermelon silver mottle virus and Peanut bud necrosis virus (57%) and a distant relationship to Peanut yellow spot virus (23%) and Peanut chlorotic fanspot virus (22%).
Cortez
I
I
Laboratory of Virology, Wageningen University, Wageningen, The Netherlands.
Saaijer
J
J
Wongjkaew
K S
KS
Pereira
A M
AM
Goldbach
R
R
Peters
D
D
Kormelink
R
R
D000595:Amino Acid Sequence; D001483:Base Sequence; D019143:Evolution, Molecular; D008969:Molecular Sequence Data; D008970:Molecular Weight; D019590:Nucleocapsid Proteins / Q000737:chemistry / Q000235:genetics*; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D019657:Solanaceae / Q000821:virology*; D013045:Species Specificity; D013785:Thailand; D017865:Tospovirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification
D019590:Nucleocapsid Proteins; D012367:RNA, Viral