title: Antibody masking renders HIV-1 resistant to cationic membrane filtration through alteration of its electrostatic characteristics.
abstract: Previously, it was demonstrated that any human immunodeficiency virus type 1 (HIV-1) strain proliferating in peripheral blood mononuclear cells (PBMCs) in vitro, and resuspended in seronegative plasma, could be captured efficiently (mean > 95%) by a porous polypropylene (PP) membrane modified cationically. We investigated if this cationic membrane could capture HIV-1 obtained from seropositive plasma, and confirmed whether this membrane was effective for the preparation of safe plasma products against HIV-1 transmission. Thirty-six seropositive plasma samples derived from HIV-1 positive cohorts in New York and Lusaka (Republic of Zambia), including 18 cases of acquired immunodeficiency syndrome (AIDS) related complex, AIDS and five terminal cases of AIDS, were filtered through the cationic membrane to determine the reduction of RNA concentration, the gag p24 concentration, and infectious titer. Only a small reduction in RNA concentration (mean < 20%) and almost no decrease in gag concentration (mean < 2%) were obtained, despite the fact that the infectivity was eliminated entirely by the filtration. Due to the possibility that anti-HIV-1 antibodies in patients' plasma combine with HIV-1, laboratory-adapted HIV-1(HTLV-IIIB) was mixed with seropositive plasma to test the effect of antibodies on HIV-1 adsorption, and also to investigate the interfacial electrokinetic potential (zeta-potential) of both intact and plasma-treated HIV-1. The zeta-potential of HIV-1(HTLV-IIIB) in the presence of seropositive plasma was neutral as opposed to negative when stored in seronegative plasma or culture medium. Also the rate of HIV-1 capture by the membrane, as determined by the reduction in RNA concentration, sank from 95% to 20%, the same capture percentage observed when filtering plasma of patients. These findings suggested that in patients' plasma, the antibody-masked HIV-1 comprise most of the viral population, and was not trapped on the cationic membrane because of its electrostatic character. Conversely, the cationic membrane was thought to adsorb antibody-free HIV-1 exclusively. It was suggested that each viral swarm had its own zeta-potential, and this difference in electrostatic character determined the extent of the viral adsorption by the cationic membrane.
authors.affiliation: Department of New Materials Section, Terumo Research and Development Center, Hadano, 259-0151, Kanagawa, Japan. JZG00166@nifty.ne.jp
authors.forename: T
authors.initials: T
authors.lastname: Owada
authors.forename: T
authors.initials: T
authors.lastname: Motomura
authors.forename: Y
authors.initials: Y
authors.lastname: Miyashita-Ogawa
authors.forename: M
authors.initials: M
authors.lastname: Kawada-Homma
authors.forename: M
authors.initials: M
authors.lastname: Onishi
authors.forename: P
authors.initials: P
authors.lastname: Matondo
authors.forename: H
authors.initials: H
authors.lastname: Terunuma
authors.forename: Y
authors.initials: Y
authors.lastname: Numazaki
authors.forename: S
authors.initials: S
authors.lastname: Yamashita
authors.forename: N
authors.initials: N
authors.lastname: Yamamoto
mesh_terms: D002412:Cations; D015483:HIV Antibodies / Q000276:immunology*; D015497:HIV-1 / Q000235:genetics / Q000276:immunology* / Q000502:physiology; D006801:Humans; D008567:Membranes, Artificial; D011094:Polyethyleneimine; D011126:Polypropylenes; D012367:RNA, Viral / Q000097:blood; D055672:Static Electricity; D019562:Viral Load
chemical_list: D002412:Cations; D015483:HIV Antibodies; D008567:Membranes, Artificial; D011126:Polypropylenes; D012367:RNA, Viral; D011094:Polyethyleneimine