[Detection of ET-1, TNF-alpha, oxidative radicals in sera of asthmatics and in sera and lung tissue of asthmatic guinea pigs].
OBJECTIVE
To investigate the role of the ET-1, TNF-alpha and oxidative radicals of MDA and SOD in the pathogenesis of asthma and the effect of the nitric oxide synthase inhibitor, L-NAME to above factors.


METHODS
ET-1 was detected by the radio-immunoassay and TNF-alpha was detected by ELISA, SOD and MDA were detected by using thiobarbituric acid method.


RESULTS
The contents of ET-1, TNF(and MDA in the serum of the asthmatic patients and in the serum and lung tissue of asthma models of guinea pigs were significantly increased, in the contrast, the content of SOD was decreased compared with those in the controls(P < 0.01). All the changes in the serum and in the lung tissues of the asthma models of guinea pigs returned to the levels of the controls by dexamethasone inhalation or L-NAME by intraperitoneal injection.


CONCLUSIONS
ET-1, TNF alpha and the oxidative radicals and NO may play important roles in the pathogenesis of asthma, L-NAME, which is similar to the dexathamethsone, may either decrease the content of NO or decrease the content of ET-1, TNF alpha and oxidative radicals in asthma.
Du
J
J
Division of Respiratory Medicine, 304 Hospital, Beijing 100037.
Cui
D
D
Guo
Y
Y
D000328:Adult; D000818:Animals; D001249:Asthma / Q000097:blood* / Q000139:chemically induced; D019332:Endothelin-1 / Q000097:blood*; D005260:Female; D006168:Guinea Pigs; D006801:Humans; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008875:Middle Aged; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors; D010047:Ovalbumin; D011897:Random Allocation; D013482:Superoxide Dismutase / Q000097:blood; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D019332:Endothelin-1; D014409:Tumor Necrosis Factor-alpha; D008315:Malondialdehyde; D010047:Ovalbumin; D019001:Nitric Oxide Synthase; D013482:Superoxide Dismutase; D019331:NG-Nitroarginine Methyl Ester