title: Indirect laser-induced fluorescence detection for capillary electrophoresis using a violet diode laser.
abstract: The violet (415 nm) diode laser is used for indirect laser-induced fluorescence detection in capillary electrophoretic separations of inorganic anions and chemical warfare agent degradation products. Inorganic anions were detected using 8-hydroxypyrene-1,3,6-trisulfonic acid as the indirect probe and achieved submicromolar (40-80 ppb) detection limits in a 2-min separation. The chemical warfare agent degradation products methylphosphonic acid, ethyl methylphosphonate, isopropyl methylphosphonate, and pinacolyl methylphosphonate were detected using the porphyrin tetrakis(4-sulfophenyl)porphine as the indirect probe and achieved detection limits of 0.1 microM (9 ppb), which are 1 order of magnitude better than that achieved using indirect UV detection. Baseline stability achieved with the violet diode laser was excellent, with dynamic reserve (DR) values of > 1000, which are 15 times better than that achieved using an unstabilized HeCd laser.
authors.affiliation: Department of Chemistry, University of Alberta, Edmonton, Alberta, T6G 2G2 Canada.
authors.forename: J E
authors.initials: JE
authors.lastname: Melanson
authors.forename: C A
authors.initials: CA
authors.lastname: Boulet
authors.forename: C A
authors.initials: CA
authors.lastname: Lucy