Peptide synthesis in organic media with subtilisin 72 immobilized on poly(vinyl alcohol)-cryogel carrier.
Serine proteinase subtilisin 72 was covalently attached to the beads of poly(vinyl alcohol)-cryogel, a macroporous hydrogel prepared by the freeze-thaw technique. The immobilized enzyme was examined as a catalyst in the synthesis of protected peptides Z-Ala-Ala-Xaa-Phe-pNA (Xaa = Leu, Glu, Lys) in acetonitrile/dimethylformamide mixtures. Immobilized subtilisin catalyzed with high yield the formation of peptide bonds between Phe-pNA and acyl donors including those with free carboxylic group and non-protected C-terminal basic and acidic amino acid residues.
Bacheva
A V
AV
Department of Chemistry of Natural Compounds, Chemical Faculty, M. V. Lomonosov Moscow State University, Russia.
Plieva
F M
FM
Lysogorskaya
E N
EN
Filippova IYu
Lozinsky
V I
VI
D000097:Acetonitriles / Q000737:chemistry / Q000494:pharmacology*; D001412:Bacillus subtilis / Q000201:enzymology; D002384:Catalysis; D004126:Dimethylformamide / Q000737:chemistry / Q000494:pharmacology*; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry; D010452:Peptide Biosynthesis; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism; D011142:Polyvinyl Alcohol / Q000737:chemistry; D012697:Serine Endopeptidases / Q000187:drug effects / Q000378:metabolism; D013381:Subtilisins / Q000187:drug effects* / Q000378:metabolism*; D013997:Time Factors
D000097:Acetonitriles; D004800:Enzymes, Immobilized; D010455:Peptides; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D004126:Dimethylformamide; D011142:Polyvinyl Alcohol; D012697:Serine Endopeptidases; D013381:Subtilisins; C032159:acetonitrile