Negative regulation of YY1 transcription factor on the dynamin I gene promoter.
Dynamin I is highly expressed in brain and plays a critical role in clathrin-mediated endocytosis and synaptic vesicle recycling. To elucidate the molecular mechanism by which expression of dynamin I is tissue-specifically regulated, we previously cloned and characterized the promoter of the mouse dynamin I gene and suggested that there is a negative regulatory element in this promoter region. In the present study, we showed that YY1 binds to this negative regulatory element located at -111 to -107 by using the EMSA and supershift analyses. Cotransfection experiment using an YY1 expression vector revealed that YY1 exerts a repressive role on the dynamin I gene promoter activity. These results demonstrate that transcription factor YY1 negatively regulates dynamin I expression via binding to the negative regulatory element.
Yoo
J
J
Cell Biology Laboratory, Korea Research Institute of Bioscience and Biotechnology, Taejon, Yusung, 305-600, Korea.
Jeong
M J
MJ
Lee
S S
SS
Lee
K I
KI
Kwon
B M
BM
Park
Y M
YM
Han
M Y
MY
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D001667:Binding, Competitive; D002460:Cell Line; D004247:DNA / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism*; D034284:Dynamin I; D034281:Dynamins; D050981:Erythroid-Specific DNA-Binding Factors; D020558:GTP Phosphohydrolases / Q000235:genetics*; D005786:Gene Expression Regulation; D051379:Mice; D011401:Promoter Regions, Genetic; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism*; D014162:Transfection; D050986:YY1 Transcription Factor
D004268:DNA-Binding Proteins; D050981:Erythroid-Specific DNA-Binding Factors; D014157:Transcription Factors; D050986:YY1 Transcription Factor; C494699:Yy1 protein, mouse; D004247:DNA; D034284:Dynamin I; D020558:GTP Phosphohydrolases; D034281:Dynamins