PCR detection method of Clostridium scindens and C. hiranonis in human fecal samples.
Clostridium scindens and C. hiranonis have high bile acid 7alpha-dehydroxylating activity. Direct PCR and nested PCR with the specific primers for C. scindens and C. hiranonis were developed and tested for the detection of them in human fecal samples. Nested PCR detected C. scindens in all samples, but direct PCR detected it in 27 of 34 samples. Regarding C. hiranonis, nested PCR detected it in only 18 of 34 samples although nested PCR has a high level of sensitivity. Nested PCR was a specific and sensitive method for the detection of C. scindens and C. hiranonis in fecal samples.
Kitahara
M
M
Japan Collection of Microorganisms, RIKEN, Wako, Saitama. kitahara@jcm.riken.go.jp
Sakamoto
M
M
Benno
Y
Y
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D003013:Clostridium / Q000235:genetics / Q000302:isolation & purification*; D017931:DNA Primers / Q000235:genetics; D005243:Feces / Q000382:microbiology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods*; D011237:Predictive Value of Tests
D017931:DNA Primers