Pretreatment with urea-hydrochloric acid enhances the isolation of Helicobacter pylori from contaminated specimens.
Human saliva seeded with H. pylori was incubated in urea-HCl and then cultured on nonselective media. Pretreatment with 0.06 N HCl-0.08 M urea for 5 min at 37 degrees C resulted in reproducible isolation of H. pylori, even at low inocula (< or =10(2) CFU/ml of saliva), despite the presence of large numbers of contaminating organisms.
Song
Q
Q
Foodborne and Diarrheal Diseases Branch, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.
Zirnstein
G W
GW
Swaminathan
B
B
Gold
B D
BD
D016481:Helicobacter Infections / Q000382:microbiology; D016480:Helicobacter pylori / Q000302:isolation & purification*; D006801:Humans; D006851:Hydrochloric Acid / Q000494:pharmacology*; D015203:Reproducibility of Results; D012463:Saliva / Q000382:microbiology*; D013048:Specimen Handling / Q000379:methods*; D014508:Urea / Q000494:pharmacology*
D014508:Urea; D006851:Hydrochloric Acid