title: [Continuously perfused cultivation of genetically-engineered CHO cells producing prothrombin in a modified Super-Spinner].
abstract: A Super-Spinner was Modified by mounting a stainless steel filter(pore size 75 microns) to the impeller shaft to retain cells while fresh nutrient is perfused. Using Macroporous microcarrier Cytopore 1, continuously perfused cultivation of a recombinant CHO cell line, CHO2DS producing prothrombin was performed with the perfusion of a protein-free medium DF6S. The cell retention rate was more than 90% during the 24 days continuously perfused cultivation. The viable cell density of CHO2DS and prothrombin concentration reached 4.62 x 10(6)(cells.m/L) and 11.3(mg/L) respectively after 9 days culture.
authors.affiliation: Institute of Biotechnology, Academy of Military Medical Sciences, Beijing 100071, China. chenzl@nic.bmi.ac.cn
authors.forename: Z L
authors.initials: ZL
authors.lastname: Chen
authors.forename: K
authors.initials: K
authors.lastname: Iding
authors.forename: D
authors.initials: D
authors.lastname: Lütkemeyer
authors.forename: J
authors.initials: J
authors.lastname: Lehmann
mesh_terms: D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D005818:Genetic Engineering; D010477:Perfusion; D011516:Prothrombin / Q000096:biosynthesis*; D011994:Recombinant Proteins / Q000096:biosynthesis*
chemical_list: D011994:Recombinant Proteins; D011516:Prothrombin