Expression of receptor for ecotropic murine leukemia virus on hematopoietic cells.
A fusion protein (F-SU/GFP) which is comprised of the surface (SU) subunit of the Friend MuLV envelope glycoprotein and the green fluorescence protein (GFP) was generated by a baculovirus expression system. The F-SU/GFP specifically bound to mammalian tissue cultured cells expressing the mCAT-1, the receptor for ecotropic murine leukemia virus (ECO-MuLV). The expression level of mCAT-1 on hematopoietic cells was measured based on the capacity of cells to absorb the F-SU/GFP. In BALB/c mice susceptible to Eco-MuLV infection, all hematopoietic cell subpopulations absorbed the F-SU/GFP with the most prominent absorption observed in the TER119+ erythroblasts. Hematopoietic cells from C4W and AKR mice did not absorb the F-SU/GFP as readily as the BALB/c cells, probably due to expression of the envelope glycoproteins of endogenous Eco-MuLVs in these mice. Although AKR mice are susceptible to Eco-MuLV infection while C4W mice are resistant, these mice showed no apparent difference in the F-SU/GFP-absorbing capacity.
Suzuki
T
T
Laboratory of Immunogenetics, National Institute of Animal Health, Tsukuba, Ibaraki, Japan.
Aizawa
S
S
Ikeda
H
H
D026923:Amino Acid Transport Systems, Basic; D000818:Animals; D016367:Baculoviridae / Q000235:genetics; D002352:Carrier Proteins / Q000378:metabolism*; D002460:Cell Line; D004900:Erythroblasts / Q000378:metabolism*; D005622:Friend murine leukemia virus / Q000378:metabolism*; D005822:Genetic Vectors; D008562:Membrane Glycoproteins / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D008806:Mice, Inbred AKR; D008807:Mice, Inbred BALB C; D011485:Protein Binding; D011991:Receptors, Virus / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D014759:Viral Envelope Proteins / Q000235:genetics / Q000378:metabolism*; D014760:Viral Fusion Proteins / Q000378:metabolism
D026923:Amino Acid Transport Systems, Basic; D002352:Carrier Proteins; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D011991:Receptors, Virus; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins; D014760:Viral Fusion Proteins; C070506:ecotropic murine leukemia virus receptor