Synthesis and characterisation of dextran and pullulan sulphate.
Dextrans and pullulans of different molar masses in the range of 10(4)-10(5) g/mol were sulphated via a SO3-pyridine complex. The degree of substitution achieved was DS = 2.4 and DS = 1.4 for dextran sulphate and DS = 2.0 and DS = 1.4 for pullulan sulphate, respectively. Confirmation of sulphation was given by FTIR spectroscopy. Asymmetrical S=O and symmetrical C-O-S stretching vibrations were detected at 1260 and 820 cm(-1). Reactivity of the polysaccharide C-atoms was determined by 13C NMR spectroscopy: For dextran this was C-3 > C-2 > C-4, while for pullulan it was C-6 > C-3 > C-2 > C-4.
Mähner
C
C
Department of Physical Chemistry, University of Osnabrück, Germany.
Lechner
M D
MD
Nordmeier
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E
D016264:Dextran Sulfate / Q000138:chemical synthesis* / Q000737:chemistry; D005936:Glucans / Q000138:chemical synthesis* / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D017550:Spectroscopy, Fourier Transform Infrared
D005936:Glucans; C009109:pullulan; D016264:Dextran Sulfate