title: Involvement of the Arg-Asp-His catalytic triad in enzymatic cleavage of the phosphodiester bond.
abstract: Phosphatidylinositol-specific phospholipase C (PI-PLC) catalyzes the cleavage of the P-O bond in phosphatidylinositol via intramolecular nucleophilic attack of the 2-hydroxyl group of inositol on the phosphorus atom. Our earlier stereochemical and site-directed mutagenesis studies indicated that this reaction proceeds by a mechanism similar to that of RNase A, and that the catalytic site of PI-PLC consists of three major components analogous to those observed in RNase A, the His32 general base, the His82 general acid, and Arg69 acting as a phosphate-activating residue. In addition, His32 is associated with Asp274 in forming a catalytic triad with inositol 2-hydroxyl, and His82 is associated with Asp33 in forming a catalytic diad. The focus of this work is to provide a global view of the mechanism, assess cooperation between various catalytic residues, and determine the origin of enzyme activation by the hydrophobic leaving group. To this end, we have investigated kinetic properties of Arg69, Asp33, and His82 mutants with phosphorothioate substrate analogues which feature leaving groups of varying hydrophobicity and pK(a). Our results indicate that interaction of the nonbridging pro-S oxygen atom of the phosphate group with Arg69 is strongly affected by Asp33, and to a smaller extent by His82. This result in conjunction with those obtained earlier can be rationalized in terms of a novel, dual-function triad comprised of Arg69, Asp33, and His82 residues. The function of this triad is to both activate the phosphate group toward the nucleophilic attack and to protonate the leaving group. In addition, Asp33 and His82 mutants displayed much smaller degrees of activation by the fatty acid-containing leaving group as compared to the wild-type (WT) enzyme, and the level of activation was significantly reduced for substrates featuring the leaving group with low pK(a) values. These results strongly suggest that the assembly of the above three residues into the fully catalytically competent triad is controlled by the hydrophobic interactions of the enzyme with the substrate leaving group.
authors.affiliation: Department of Medicinal Chemistry and Pharmacognosy, University of Illinois at Chicago, 60612, USA.
authors.forename: R J
authors.initials: RJ
authors.lastname: Kubiak
authors.forename: X
authors.initials: X
authors.lastname: Yue
authors.forename: R J
authors.initials: RJ
authors.lastname: Hondal
authors.forename: C
authors.initials: C
authors.lastname: Mihai
authors.forename: M D
authors.initials: MD
authors.lastname: Tsai
authors.forename: K S
authors.initials: KS
authors.lastname: Bruzik
mesh_terms: D019943:Amino Acid Substitution / Q000235:genetics; D001120:Arginine / Q000737:chemistry* / Q000235:genetics; D001216:Asparagine / Q000737:chemistry* / Q000235:genetics; D001409:Bacillus cereus / Q000201:enzymology; D001665:Binding Sites / Q000235:genetics; D002384:Catalysis; D020134:Catalytic Domain / Q000235:genetics; D006639:Histidine / Q000737:chemistry* / Q000235:genetics; D006868:Hydrolysis; D007295:Inositol Phosphates / Q000737:chemistry; D010755:Organophosphates / Q000737:chemistry*; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D054801:Phosphoinositide Phospholipase C; D012259:Ribonuclease, Pancreatic / Q000737:chemistry; D013025:Glycine max / Q000201:enzymology; D013329:Structure-Activity Relationship; D013379:Substrate Specificity / Q000235:genetics; D013455:Sulfur / Q000737:chemistry; D013873:Thionucleotides / Q000737:chemistry; D010738:Type C Phospholipases / Q000737:chemistry* / Q000235:genetics
chemical_list: D007295:Inositol Phosphates; D010755:Organophosphates; D013873:Thionucleotides; D006639:Histidine; D001216:Asparagine; D013455:Sulfur; C091533:glycerophosphoinositol 4,5-bisphosphate; D001120:Arginine; D012259:Ribonuclease, Pancreatic; D010738:Type C Phospholipases; D054801:Phosphoinositide Phospholipase C; D043265:Phosphatidylinositol Diacylglycerol-Lyase