Piezoresponse of the cyo-operon coding for quinol oxidase subunits in a deep-sea piezophilic bacterium, Shewanella violacea.
We have isolated the genes for quinol oxidase from a deep-sea piezophilic bacterium, Shewanella violacea. Analysis of the deduced amino acid sequences of the cyo subunits showed that this oxidase has high similarity to Escherichia coli bo-type quinol oxidase. Northern blot analysis showed that these genes are expressed at a high level when the bacterium is grown at elevated pressure. Upstream in the cyo-operon, a sigma54-binding motif and an octamer sequence unit were found, suggesting that these elements may play a role in regulation of expression of the cyo-operon in response to changes in pressure.
Nakasone
K
K
The DEEPSTAR Group, Japan Marine Science and Technology Center, Yokosuka. nakasone@jamstec.go.jp
Yamada
M
M
Qureshi
M H
MH
Kato
C
C
Horikoshi
K
K
D001483:Base Sequence; D004269:DNA, Bacterial; D008969:Molecular Sequence Data; D009876:Operon; D010088:Oxidoreductases / Q000235:genetics*; D020592:Shewanella / Q000201:enzymology* / Q000235:genetics
D004269:DNA, Bacterial; D010088:Oxidoreductases; C020094:duroquinol oxidase