Characterization of the ToxB gene from Pyrenophora tritici-repentis.
The ToxB gene was cloned and characterized from a race 5 isolate of Pyrenophora tritici-repentis from North Dakota. ToxB contains a 261-bp open reading frame that encodes a 23 amino acid putative signal peptide and a 64 amino acid host-selective toxin, Ptr ToxB. Analysis of Ptr ToxB from heterologous expression in Pichia pastoris confirms that ToxB encodes a host-selective toxin.
Martinez
J P
JP
Department of Botany and Plant Pathology, Oregon State University, Corvallis 97331, USA.
Ottum
S A
SA
Ali
S
S
Franci
L J
LJ
Ciuffetti
L M
LM
D000595:Amino Acid Sequence; D001203:Ascomycota / Q000235:genetics* / Q000472:pathogenicity; D001483:Base Sequence; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010843:Pichia / Q000235:genetics; D010935:Plant Diseases / Q000382:microbiology; D021382:Protein Sorting Signals; D017422:Sequence Analysis, DNA; D020539:Sequence Analysis, Protein; D014908:Triticum / Q000382:microbiology*
D005656:Fungal Proteins; D021382:Protein Sorting Signals; C434209:ToxB protein, Pyrenophora tritici-repentis