The effects of live Streptococcus pneumoniae and tumor necrosis factor-alpha on neutrophil oxidative burst and beta 2-integrin expression.
OBJECTIVE
To study the effects of TNF-alpha and live Streptococcus pneumoniae on human neutrophil oxidative burst and beta 2-integrin expression using flow cytometry.


METHODS
Six clinical isolates of S. pneumoniae (serotypes 3, 19A, 22F, 6A, 33F and 9N) from patients with bacteremic pneumonia or upper respiratory tract infections were studied. Whole blood was incubated either alone, with TNF-alpha or with S. pneumoniae or with both TNF-alpha and pneumococci at a ratio of one neutrophil per 1--5 bacteria. After 30 min of incubation, the tubes were put into ice, fixed and analysed.


RESULTS
S. pneumoniae caused an increase in oxidative burst but not greater than that caused by TNF-alpha alone. When whole blood was preincubated with TNF-alpha for 30 min before the addition of pneumococci, a further increase in the oxidative burst response was seen. The variation in CD11b expression was not significant. Both S. pneumoniae and TNF-alpha caused increases in CD18 expression. The addition of TNF-alpha directly with the bacteria caused no further increase, but preincubation of blood with TNF-alpha 30 min before the addition of the bacteria caused a significant increase in CD18 expression.


CONCLUSIONS
Live S. pneumoniae stimulates polymorphonuclear leukocytes to produce an oxidative burst and increases expression of CD18, and these effects are enhanced by TNF-alpha.
Kragsbjerg
P
P
Department of Internal Medicine, Division of Hematology, Orebro Medical Center Hospital, Orebro, Sweden. peter.kragsbjerg@orebroll.se
Fredlund
H
H
D016470:Bacteremia / Q000382:microbiology; D018821:CD18 Antigens / Q000378:metabolism*; D002675:Child, Preschool; D006801:Humans; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D011008:Pneumococcal Infections / Q000382:microbiology; D016897:Respiratory Burst / Q000276:immunology*; D013296:Streptococcus pneumoniae / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000494:pharmacology*
D018821:CD18 Antigens; D014409:Tumor Necrosis Factor-alpha