Molecular characterization of human UDP-glucuronic acid/UDP-N-acetylgalactosamine transporter, a novel nucleotide sugar transporter with dual substrate specificity.
A novel human nucleotide sugar transporter (NST) which transports both UDP-glucuronic acid (UDP-GlcA) and UDP-N-acetylgalactosamine (UDP-GalNAc) has been identified, cloned and characterized. The strategy for the identification of the novel NST involved a search of the expressed sequence tags database for genes related to the human UDP-galactose transporter-related isozyme 1, followed by heterologous expression of a candidate gene (hUGTrel7) in Saccharomyces cerevisiae and biochemical analyses. Significantly more UDP-GlcA and UDP-GalNAc were translocated from the reaction medium into the lumen of microsomes prepared from the hUGTrel7-expressing yeast cells than into the control microsomes from cells not expressing hUGTrel7. The possibility that this transporter participates in glucuronidation and/or chondroitin sulfate biosynthesis is discussed.
Muraoka
M
M
Department of Physiological Chemistry, The Tokyo Metropolitan Institute of Medical Science (Rinshoken), 18-22 Honkomagome 3-chome, Bunkyo-ku, 113-8613, Tokyo, Japan.
Kawakita
M
M
Ishida
N
N
D000595:Amino Acid Sequence; D000818:Animals; D001427:Bacterial Toxins / Q000494:pharmacology; D001483:Base Sequence; D001692:Biological Transport / Q000187:drug effects; D016466:CHO Cells; D006224:Cricetinae; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D015870:Gene Expression; D006460:Hemolysin Proteins / Q000494:pharmacology; D006801:Humans; D008861:Microsomes / Q000378:metabolism; D008969:Molecular Sequence Data; D009004:Monosaccharide Transport Proteins / Q000235:genetics* / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013379:Substrate Specificity / Q000502:physiology; D014170:Transformation, Genetic; D022161:Transport Vesicles / Q000378:metabolism; D014535:Uridine Diphosphate Glucuronic Acid / Q000378:metabolism* / Q000493:pharmacokinetics; D014536:Uridine Diphosphate N-Acetylgalactosamine / Q000378:metabolism* / Q000493:pharmacokinetics
D001427:Bacterial Toxins; D018076:DNA, Complementary; D006460:Hemolysin Proteins; D009004:Monosaccharide Transport Proteins; C425807:SLC35D1 protein, human; C021091:staphylococcal alpha-toxin; D014535:Uridine Diphosphate Glucuronic Acid; D014536:Uridine Diphosphate N-Acetylgalactosamine