title: Isolation of a family of organic anion transporters from human liver and kidney.
abstract: Five distinct organic anion transporter cDNAs, hOAT1-5, were isolated from human liver and kidney. hOAT1, 2, and 3 are homologous to their respective rat orthologues OAT1-3, whereas hOAT4 and 5 are novel clones that have not been identified in other species. hOAT1- and hOAT3-transfected cells showed uptake of p-aminohippurate and fluorescein. Cells expressing hOAT2 showed uptake of p-aminohippurate, methotrexate, cAMP, and alpha-ketoglutarate. Northern blot analysis indicated differential tissue distribution for the transporter transcripts. These results indicate the existence of a family of organic anion transporting proteins in humans distinct from the oatp-like family of transporters.
authors.affiliation: Metabasis Therapeutics, Inc, San Diego, California 92121, USA. sun@mbasis.com
authors.forename: W
authors.initials: W
authors.lastname: Sun
authors.forename: R R
authors.initials: RR
authors.lastname: Wu
authors.forename: P D
authors.initials: PD
authors.lastname: van Poelje
authors.forename: M D
authors.initials: MD
authors.lastname: Erion
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D027321:Anion Transport Proteins; D002352:Carrier Proteins / Q000235:genetics* / Q000302:isolation & purification* / Q000378:metabolism; D002460:Cell Line; D003001:Cloning, Molecular; D000242:Cyclic AMP / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007656:Ketoglutaric Acids / Q000378:metabolism; D007668:Kidney / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008727:Methotrexate / Q000378:metabolism; D008969:Molecular Sequence Data; D009865:Oocytes / Q000378:metabolism; D027361:Organic Anion Transporters; D029362:Organic Anion Transporters, Sodium-Independent; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D014162:Transfection; D014982:Xenopus laevis; D010130:p-Aminohippuric Acid / Q000378:metabolism
chemical_list: D027321:Anion Transport Proteins; D002352:Carrier Proteins; D018076:DNA, Complementary; D007656:Ketoglutaric Acids; D027361:Organic Anion Transporters; D029362:Organic Anion Transporters, Sodium-Independent; D012333:RNA, Messenger; C427208:SLC22A10 protein, human; C427207:SLC22A11 protein, human; D000242:Cyclic AMP; D010130:p-Aminohippuric Acid; D008727:Methotrexate