Determination of idebenone in plasma by HPLC/MS.
OBJECTIVE
To develop a sensitive method for the analysis of idebenone [Ide, 6-(10-hydroxydecyl)-2,3-dimethoxy-5-methyl-p-benzoquinone] in human plasma.


METHODS
The concentrations in plasma were determined by a high performance liquid chromatography-mass spectrometry coupled with an atmosphere pressure chemical impact ion source method (LC/MS-APCI).


RESULTS
The chromatograms indicated a good separation of the analytes and there was no interference by other compounds. The coefficients of determination of the calibration curves were above 0.999. The calibration range was 20-600 micrograms.L-1. Peak plasma idebenone concentration (Cmax) of (316 +/- 85) micrograms.L-1 was achieved within (96 +/- 34) min (Tpeak) after an oral dose of 30 mg. Lower limit of quantitation of the method for Ide determination was 20 micrograms.L-1.


CONCLUSIONS
The advantages of using LC/MS-APCI technique include better sensitivity, higher selectivity, and less time consumption, compared with HPLC method.
Hu
P
P
Clinical Pharmacological Research Center, Peking Union Medical College Hospital, Peking Union Medical College, Beijing 100730, China. peihu@public3.bta.net.cn
Jiang
J
J
Xie
H H
HH
D000975:Antioxidants / Q000032:analysis* / Q000494:pharmacology; D016227:Benzoquinones / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D014451:Ubiquinone / Q000031:analogs & derivatives
D000975:Antioxidants; D016227:Benzoquinones; D014451:Ubiquinone; C036619:idebenone