title: A possible solvent effect of adenosine diphosphate influences the binding of 1,N6 ethenoadenosine diphosphate to myosin from skeletal muscle.
abstract: Skeletal muscle myosin displays two independent and equivalent binding sites for 1,N6 ethenoadenosine diphosphate, with a dissociation constant of 24.7 microM. MgADP, 10 to 40 microM, behaves as a pure competitive type inhibitor (K(SI)=8-9 microM) for the binding of 1,N6 ethenoadenosine diphosphate to skeletal muscle myosin. On the contrary, the inhibition by MgADP, 0.11-1.54 mM, is neither competitive nor non-competitive nor mixed, as is revealed by the analysis with the general kinetic equation (K.J. Laidler, P.S. Bunting, The Chemical Kinetics of Enzyme Action, 2nd ed., Clarendon, Oxford, 1973, p. 94). To explain our finding we propose that MgADP operates a complex type of inhibition, acting both directly as a competitor for myosin active sites, and indirectly by perturbing the regions of the solvent near to the protein.
authors.affiliation: Dipartimento di Biochimica e Biologia Molecolare, Università di Ferrara, Via Borsari 46, 44100, Ferrara, Italy. gre@ifeuniv.unife.it
authors.forename: E
authors.initials: E
authors.lastname: Grazi
authors.forename: O
authors.initials: O
authors.lastname: Cintio
authors.forename: E
authors.initials: E
authors.lastname: Magri
authors.forename: G
authors.initials: G
authors.lastname: Trombetta
mesh_terms: D020106:Acrylamide; D000244:Adenosine Diphosphate / Q000031:analogs & derivatives* / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites; D001667:Binding, Competitive; D066298:In Vitro Techniques; D007700:Kinetics; D018482:Muscle, Skeletal / Q000378:metabolism; D009218:Myosins / Q000378:metabolism*; D011122:Polyphosphates / Q000494:pharmacology; D011485:Protein Binding; D011817:Rabbits; D012997:Solvents; D013050:Spectrometry, Fluorescence
chemical_list: D011122:Polyphosphates; D012997:Solvents; D020106:Acrylamide; C015195:1,N(6)-ethenoadenosine diphosphate; D000244:Adenosine Diphosphate; D009218:Myosins; C009293:trimetaphosphoric acid