Investigation of varicella-zoster virus DNA in lymphocyte subpopulations by quantitative PCR assay.
To investigate the nature of viremia during the acute phase of varicella, we studied the viral load in nine otherwise healthy children with varicella. Plasma and peripheral blood mononuclear cells (PBMC) were obtained, then PBMC were divided into CD4+T, CD8+T, and B lymphocytes and monocyte/macrophage fractions. The viral DNA in each component was quantified using a real-time quantitative polymerase chain reaction assay. Varicella-zoster virus (VZV) DNA was detected in plasma, PBMC and all subpopulations. The amount of viral DNA was similar in each PBMC subpopulation, suggesting that each lymphocyte fraction and monocytes carry similar amounts of VZV DNA during viremia.
Ito
Y
Y
Department of Pediatrics/Developmental Pediatrics, Nagoya University Graduate School of Medicine, Aichi, Japan. yyito@med.nagoya-u.ac.jp
Kimura
H
H
Hara
S
S
Kido
S
S
Ozaki
T
T
Nishiyama
Y
Y
Morishima
T
T
D000818:Animals; D002644:Chickenpox / Q000097:blood*; D002645:Chickens; D004279:DNA, Viral / Q000097:blood*; D014645:Herpesvirus 3, Human / Q000235:genetics / Q000302:isolation & purification*; D016131:Lymphocyte Subsets / Q000821:virology*; D016133:Polymerase Chain Reaction / Q000379:methods; D019562:Viral Load
D004279:DNA, Viral