title: Resuscitation with room air instead of 100% oxygen prevents oxidative stress in moderately asphyxiated term neonates.
abstract: BACKGROUND
  Traditionally, asphyxiated newborn infants have been ventilated using 100% oxygen. However, a recent multinational trial has shown that the use of room air was just as efficient as pure oxygen in securing the survival of severely asphyxiated newborn infants. Oxidative stress markers in moderately asphyxiated term newborn infants resuscitated with either 100% oxygen or room air have been studied for the first time in this work.
  
  
  METHODS
  Eligible term neonates with perinatal asphyxia were randomly resuscitated with either room air or 100% oxygen. The clinical parameters recorded were those of the Apgar score at 1, 5, and 10 minutes, the time of onset of the first cry, and the time of onset of the sustained pattern of respiration. In addition, reduced and oxidized glutathione concentrations and antioxidant enzyme activities (superoxide dismutase, catalase, and glutathione peroxidase) were determined in blood from the umbilical artery during delivery and in peripheral blood at 72 hours and at 4 weeks' postnatal age.
  
  
  RESULTS
  Our results show that the room-air resuscitated (RAR) group needed significantly less time to first cry than the group resuscitated with 100% oxygen (1.2 +/- 0.6 minutes vs 1.7 +/- 0.5). Moreover, the RAR group needed less time undergoing ventilation to achieve a sustained respiratory pattern than the group resuscitated with pure oxygen (4.6 +/- 0.7 vs 7.5 +/- 1.8 minutes). The reduced-to-oxidized-glutathione ratio, which is an accurate index of oxidative stress, of the RAR group (53 +/- 9) at 28 days of postnatal life showed no differences with the control nonasphyxiated group (50 +/- 12). However, the reduced-to-oxidized-glutathione ratio of the 100% oxygen-resuscitated group (OxR) (15 +/- 5) was significantly lower and revealed protracted oxidative stress. Furthermore, the activities of superoxide dismutase and catalase in erythrocytes were 69% and 78% higher, respectively, in the OxR group than in the control group at 28 days of postnatal life. Thus, this shows that these antioxidant enzymes, although higher than in controls, could not cope with the ongoing generation of free radicals in the OxR group. However, there were no differences in antioxidant enzyme activities between the RAR group and the control group at this stage.
  
  
  CONCLUSIONS
  There are no apparent clinical disadvantages in using room air for ventilation of asphyxiated neonates rather than 100% oxygen. Furthermore, RAR infants recover more quickly as assessed by Apgar scores, time to the first cry, and the sustained pattern of respiration. In addition, neonates resuscitated with 100% oxygen exhibit biochemical findings reflecting prolonged oxidative stress present even after 4 weeks of postnatal life, which do not appear in the RAR group. Thus, the current accepted recommendations for using 100% oxygen in the resuscitation of asphyxiated newborn infants should be further discussed and investigated.
authors.affiliation: Servicio de Pediatría, Hospital Virgen del Consuelo, Valencia, Spain. maximo.vento@uv.es
authors.forename: M
authors.initials: M
authors.lastname: Vento
authors.forename: M
authors.initials: M
authors.lastname: Asensi
authors.forename: J
authors.initials: J
authors.lastname: Sastre
authors.forename: F
authors.initials: F
authors.lastname: García-Sala
authors.forename: F V
authors.initials: FV
authors.lastname: Pallardó
authors.forename: J
authors.initials: J
authors.lastname: Viña
mesh_terms: D000388:Air; D001034:Apgar Score; D001238:Asphyxia Neonatorum / Q000175:diagnosis / Q000378:metabolism / Q000628:therapy*; D006801:Humans; D007231:Infant, Newborn; D018384:Oxidative Stress; D010102:Oxygen Inhalation Therapy; D012138:Respiratory Therapy / Q000379:methods*; D012151:Resuscitation / Q000379:methods*; D012720:Severity of Illness Index; D016896:Treatment Outcome