Detection of antibodies to Babesia equi in horses by a latex agglutination test using recombinant EMA-1.
A latex agglutination test (LAT) using recombinant equi merozoite antigen 1 (EMA-1) for the detection of antibodies to Babesia equi was developed. The LAT was able to differentiate very clearly between sera from B. equi-infected horses and sera from Babesia caballi-infected horses or from normal horses. The LAT results were identical to those of a previously developed enzyme-linked immunosorbent assay. These results indicate that LAT using recombinant EMA-1 might be very useful as a routine screening method for the diagnosis of B. equi infection.
Xuan
X
X
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan. gen@obihiro.ac.jp
Igarashi
I
I
Tanaka
T
T
Fukumoto
S
S
Nagasawa
H
H
Fujisaki
K
K
Mikami
T
T
D000818:Animals; D000913:Antibodies, Protozoan / Q000276:immunology*; D000953:Antigens, Protozoan / Q000235:genetics / Q000276:immunology; D001403:Babesia / Q000276:immunology*; D001404:Babesiosis / Q000175:diagnosis / Q000276:immunology* / Q000469:parasitology; D006736:Horses; D007841:Latex Fixation Tests; D008565:Membrane Proteins / Q000235:genetics / Q000276:immunology; D015800:Protozoan Proteins / Q000235:genetics / Q000276:immunology; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; C085964:EMA-1 protein, Babesia equi; D008565:Membrane Proteins; D015800:Protozoan Proteins; D011994:Recombinant Proteins