Tissue-specific in vivo inhibition of DNase I gene expression by somatostatin.
Administration of somatostatin to rats induced a transient reduction of serum levels of deoxyribonuclease I (DNase I) activity in a dose-dependent manner, followed by a substantial decrease of DNase I activity in the lower gut. Activity in the parotid gland, liver, and kidney did not change. Real-time PCR analysis of the DNase I gene transcript in ileum indicated that the decrease was due to down-regulation of gene expression. Based on these responses, rat tissues expressing DNase I could be classified into two types, somatostatin-sensitive and somatostatin-resistant, and the level of DNase I activity in the lower gut seems to be controlled by somatostatin.
Yasuda
T
T
Department of Biology, Fukui Medical University, Matsuoka, Fukui, 910-1193, Japan.
Takeshita
H
H
Ueki
M
M
Iida
R
R
Nakajima
T
T
Mori
S
S
Mogi
K
K
Kaneko
Y
Y
Kishi
K
K
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D003850:Deoxyribonuclease I / Q000097:blood / Q000235:genetics* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004351:Drug Resistance; D005260:Female; D015870:Gene Expression / Q000187:drug effects*; D007082:Ileum / Q000187:drug effects / Q000201:enzymology; D008297:Male; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013004:Somatostatin / Q000008:administration & dosage / Q000494:pharmacology*; D014018:Tissue Distribution
D017931:DNA Primers; D012333:RNA, Messenger; D013004:Somatostatin; D003850:Deoxyribonuclease I