title: Rapid, high-throughput extraction of bacterial genomic DNA from selective-enrichment culture media.
abstract: OBJECTIVE
  To create a fast, sensitive and inexpensive high-throughput method for the extraction of bacterial genomic DNA from selective-enrichment culture media.
  
  
  RESULTS
  Lysis of bacteria was achieved using guanidinium isothiocyanate, and DNA was extracted using 96-well glass microfibre filtration plates. Extraction-PCR detected the presence of 1 cfu Yersinia ruckeri and 16 cfu Lactococcus garvieae 200 microl(-1) sample of selective-enrichment medium.
  
  
  CONCLUSIONS
  An efficient method for high-throughput extraction of bacterial genomic DNA from selective-enrichment culture media was achieved.
  
  
  CONCLUSIONS
  This method enables detection of covert bacterial infections in fish. The simultaneous extraction of large numbers of samples allows for its use in bacterial monitoring programmes and quarantine.
authors.affiliation: Fish Health Unit, Tasmanian Aquaculture and Fisheries Institute, University of Tasmania, Launceston, Australia. Teresa.Wilson@dpiwe.tas.gov.au
authors.forename: T
authors.initials: T
authors.lastname: Wilson
authors.forename: J
authors.initials: J
authors.lastname: Carson
mesh_terms: D001419:Bacteria / Q000254:growth & development / Q000302:isolation & purification*; D003470:Culture Media; D004269:DNA, Bacterial / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity
chemical_list: D003470:Culture Media; D004269:DNA, Bacterial