Dysfunction of microvascular endothelial cells induced by tumor necrosis factor (TNFalpha): cellular and molecular mechanism.
The dysfunction of rat pulmonary microvascular endothelial cells (RPMVEC) induced by tumor necrosis factor alpha (TNFalpha) was investigated in cell culture. The TNFalpha concentrations added into culture were 1000-5000 u/ml. The study was done at different steps as follows: (1) cell proliferation dynamic, (2) coagulation and anti-coagulation, (3) adhesive molecule, (4) nitric oxide (NO), (5) oncogens, and (6) apoptosis. It was concluded that attention should be paid to the metabolic disorder and dysfunction of RPMVEC induced by TNFalpha which can not be found easily according to the morphological changes. This might be the common pathway in the pathogenesis of some diseases related to TNFalpha.
Jin
H M
HM
Department of Pathophysiology, Shanghai Medical University, China. hmjin@shmu.edu.cn
Liu
Q H
QH
Cao
X
X
Wu
Z H
ZH
Zhang
G P
GP
Zhang
M
M
Sha
Z Y
ZY
D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics; D002455:Cell Division; D002478:Cells, Cultured / Q000187:drug effects; D004730:Endothelium, Vascular / Q000187:drug effects*; D004790:Enzyme Induction / Q000187:drug effects; D011464:Epoprostenol / Q000096:biosynthesis / Q000235:genetics; D005353:Fibronectins / Q000096:biosynthesis / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects; D016762:Genes, fos; D008833:Microcirculation / Q000187:drug effects*; D009569:Nitric Oxide / Q000096:biosynthesis; D019001:Nitric Oxide Synthase / Q000096:biosynthesis / Q000235:genetics; D052247:Nitric Oxide Synthase Type II; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis; D011651:Pulmonary Artery / Q000166:cytology; D051381:Rats; D010959:Tissue Plasminogen Activator / Q000096:biosynthesis / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology* / Q000633:toxicity
D005353:Fibronectins; D016760:Proto-Oncogene Proteins c-fos; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; D011464:Epoprostenol; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496318:Nos2 protein, rat; D010959:Tissue Plasminogen Activator