On-line sample concentration in micellar electrokinetic chromatography with cationic micelles in a coated capillary.
The electroosmotic flow was successfully suppressed even in the presence of cationic surfactants, when a polyacrylamide-coated capillary was employed. Two on-line sample concentration techniques of sample stacking and sweeping were evaluated in micellar electrokinetic chromatography (MEKC) using the polyacrylamide-coated capillary. Cationic surfactants were used as pseudostationary phases in MEKC. At least 60-fold and about 600-fold increases in detection sensitivity were obtained in terms of peak heights by sample stacking and sweeping, respectively.
Kim
J B
JB
Faculty of Science, Himeji Institute of Technology, Kamigori, Hyogo, Japan. jbkim@sci.himeji-tech.ac.jp
Otsuka
K
K
Terabe
S
S
D000180:Acrylic Resins; D002412:Cations; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D008823:Micelles; D000099166:Polyacrylamides
D000180:Acrylic Resins; D002412:Cations; D008823:Micelles; D000099166:Polyacrylamides