Differential expression of the subunits of the glucose-6-phosphatase system in the clear cell type of human renal cell carcinoma - no evidence for an overexpression of protein kinase B.
The expression of two components of the glucose-6-phosphatase system, the catalytic subunit (G6PaseC) and the glucose-6-phosphate transporter, was analyzed in the clear cell type of human renal cell carcinoma. The expression of G6PaseC was decreased in tumours compared with non-tumourous tissue of the same patient. The expression of G6PaseT varied with no general trend between tumours and control tissue. The expression of protein kinase B (PKB) was unchanged in the tumours, suggesting that the down-regulation of G6PaseC in clear cells and the maintenance of the transformed phenotype are not predominantly caused by an overexpression of PKB.
Schmoll
D
D
Department of Medical Biochemistry and Molecular Biology, University of Greifswald, D-17487, Greifswald, Germany. schmoll@mail.uni-greifswald.de
Balabanov
S
S
Schwarck
D
D
Burchell
A
A
Kleist
B
B
Zimmermann
U
U
Walther
R
R
D000368:Aged; D017920:Antiporters / Q000096:biosynthesis* / Q000235:genetics; D015152:Blotting, Northern; D002292:Carcinoma, Renal Cell / Q000201:enzymology* / Q000235:genetics; D020134:Catalytic Domain / Q000235:genetics; D015536:Down-Regulation / Q000502:physiology; D005260:Female; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology; D005952:Glucose-6-Phosphatase / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D007680:Kidney Neoplasms / Q000201:enzymology* / Q000235:genetics; D008297:Male; D008875:Middle Aged; D009004:Monosaccharide Transport Proteins / Q000096:biosynthesis* / Q000235:genetics; D017346:Protein Serine-Threonine Kinases; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis* / Q000235:genetics; D051057:Proto-Oncogene Proteins c-akt; D020133:Reverse Transcriptase Polymerase Chain Reaction
D017920:Antiporters; D009004:Monosaccharide Transport Proteins; D011518:Proto-Oncogene Proteins; C503531:SLC37A4 protein, human; C111261:glucose 6-phosphate(transporter); D017346:Protein Serine-Threonine Kinases; D051057:Proto-Oncogene Proteins c-akt; D005952:Glucose-6-Phosphatase