title: Differential expression of growth factors in irradiated mouse testes.
abstract: OBJECTIVE
  By using as an experimental model the male mouse gonad, which contains both radiosensitive (germ) and radioresistant (somatic) cells, we have studied the growth factor (and/or receptor) expression of transforming growth factor-beta receptor (TGFbeta RI), stem cell factor (SCF), c-kit, Fas-L, Fas, tumor necrosis factor receptor (TNF R55), and leukemia inhibiting factor receptor (LIF-R) after local irradiation.
  
  
  METHODS
  Adult male mice were locally irradiated on the testes. Induction of apoptosis in the different testicular cell types following X-ray radiation was identified by the TdT-mediated dUTP Nick End Labeling (TUNEL) approach. Growth factor expression was evidenced by semiquantitative RT-PCR and Western blot analyses.
  
  
  RESULTS
  Apoptosis, identified through the TUNEL approach, occurred in radiosensitive testicular (premeotic) germ cells with the following kinetics: the number of apoptotic cells increased after 24 h (p < 0.001) and was maximal 48 h after a 2-Gy ionizing radiation (p < 0.001). Apoptotic cells were no longer observed 72 h after a 2-Gy irradiation. The number of apoptotic cells increased with the dose of irradiation (1-4 Gy). In the seminiferous tubules, the growth factor expression in premeiotic radiosensitive germ cells was modulated by irradiation. Indeed Fas, c-kit, and LIF-R expression, which occurs in (radiosensitive) germ cells, decreased 24 h after a 2-Gy irradiation, and the maximal decrease was observed with a 4-Gy irradiation. The decrease in Stra8 expression occurred earlier, at 4 h after a 2-Gy irradiation. In addition, a significant (p < 0.03) decrease in Stra8 mRNA levels was observed at the lowest dose used (0.5 Gy, 48 h). Moreover, concerning a growth factor receptor, such as TGFbeta RI, which is expressed both in radiosensitive and radioresistant cells, we observed a differential expression depending on the cell radiosensitivity after irradiation. Indeed, TGFbeta RI expression was increased after irradiation in interstitial radioresistant testicular cells in a dose- and time-dependent manner, while it decreased in seminiferous radiosensitive (germ cells) testicular cells. Such a differential expression between radioresistant and radiosensitive cells in TGFbeta RI levels was observed in terms of both mRNA and protein. In contrast, the growth factors specifically expressed in the somatic radioresistant (Sertoli) cells in the seminiferous tubules (SCF, Fas-L, TNF R55) were not affected by ionizing radiation (up to 4 Gy, 72 h).
  
  
  CONCLUSIONS
  Growth factor expression decreased in the radiosensitive testicular cells after irradiation. Such a decrease occurred before the detection of apoptosis using the TUNEL approach. TGFbeta RI mRNA levels decreased in the radiosensitive cells, whereas it increased in the radioresistant cells, suggesting that TGFbeta RI may represent a biomarker of the intrinsic radiosensitivity of cells.
authors.affiliation: Laboratoire de Recherche sur les Communications Cellulaires en Biologie de la Reproduction, Institut National de la Santé et de la Recherche Médicale, Faculté de Médecine Lyon-Sud, BP 12, 69 921 Oullins cedex, France. mauduit@lsgrisn1.univ-lyon1.fr
authors.forename: C
authors.initials: C
authors.lastname: Mauduit
authors.forename: A
authors.initials: A
authors.lastname: Siah
authors.forename: M
authors.initials: M
authors.lastname: Foch
authors.forename: O
authors.initials: O
authors.lastname: Chapet
authors.forename: S
authors.initials: S
authors.lastname: Clippe
authors.forename: J P
authors.initials: JP
authors.lastname: Gerard
authors.forename: M
authors.initials: M
authors.lastname: Benahmed
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000528:radiation effects; D006133:Growth Substances / Q000096:biosynthesis*; D008297:Male; D051379:Mice; D011836:Radiation Tolerance / Q000502:physiology; D017978:Receptors, Growth Factor / Q000096:biosynthesis; D018125:Receptors, Transforming Growth Factor beta / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012708:Sertoli Cells / Q000378:metabolism / Q000528:radiation effects; D013094:Spermatozoa / Q000378:metabolism / Q000528:radiation effects; D013737:Testis / Q000166:cytology / Q000378:metabolism* / Q000528:radiation effects*
chemical_list: D006133:Growth Substances; D017978:Receptors, Growth Factor; D018125:Receptors, Transforming Growth Factor beta