[The different responses to anoxia in cultured CA1 and DG neurons from newborn rats].
Tissue culture from hippocampal CA1 or dentate gyrus (DG) region was established on the basis of previous neuronal culture technique. The viability, intracellular calcium concentration and brain-derived neurotrophic factor (BDNF) mRNA expression of the two kinds of neurons after anoxia were observed in the counting in the confocal microscopic field and in situ hybridization. It is found that DG neurons are not only more resistant to anoxia, but also have a stronger ability to keep calcium homeostasis and to express BDNF mRNA than CA1 neurons.
Yao
H
H
Institute of Basic Medical Sciences, Beijing 100850.
Huang
Y H
YH
Liu
Z W
ZW
Wan
Q
Q
Ding
A S
AS
Zhao
B
B
Fan
M
M
Wang
F Z
FZ
D000818:Animals; D000831:Animals, Newborn; D019208:Brain-Derived Neurotrophic Factor / Q000096:biosynthesis* / Q000235:genetics; D015687:Cell Hypoxia; D002470:Cell Survival; D002478:Cells, Cultured; D018891:Dentate Gyrus / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D006624:Hippocampus / Q000166:cytology / Q000502:physiology*; D009474:Neurons / Q000166:cytology; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar
D019208:Brain-Derived Neurotrophic Factor; D012333:RNA, Messenger