title: Inactivation of thrombin by a fucosylated chondroitin sulfate from echinoderm.
abstract: A polysaccharide extracted from the sea cucumber body wall has the same backbone structure as the mammalian chondroitin sulfate, but some of the glucuronic acid residues display sulfated fucose branches. These branches confer high anticoagulant activity to the polysaccharide. Since the sea cucumber chondroitin sulfate has analogy in structure with mammalian glycosaminoglycans and sulfated fucans from brown algae, we compared its anticoagulant action with that of heparin and of a homopolymeric sulfated fucan with approximately the same level of sulfation as the sulfated fucose branches found in the sea cucumber polysaccharide. These various compounds differ not only in their anticoagulant potencies but also in the mechanisms of thrombin inhibition. Fucosylated chondroitin sulfate, like heparin, requires antithrombin or heparin cofactor II for thrombin inhibition. Sulfated fucans from brown algae have an antithrombin effect mediated by antithrombin and heparin cofactor II, plus a direct antithrombin effect more pronounced for some fractions. But even in the case of these two polysaccharides, we observed some differences. In contrast with heparin, total inhibition of thrombin in the presence of antithrombin is not achieved with fucosylated chondroitin sulfate, possibly reflecting a less specific interaction. Fucosylated chondroitin sulfate is able to inhibit thrombin generation after stimulation by both contact-activated and thromboplastin-activated systems. It delayed only the contact-induced thrombin generation, as expected for an anticoagulant without direct thrombin inhibition. Overall, the specific spatial array of the sulfated fucose branches in the fucosylated chondroitin sulfate not only confer high anticoagulant activity to the polysaccharide but also determine differences in the way it inhibits thrombin.
authors.affiliation: Hospital Universitário Clementino Fraga Filho and Departamento de Bioquímica Médica, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Caixa Postal 68041, RJ 21941-590, Rio de Janeiro, Brazil. pmourao@hucff.ufrj.br
authors.forename: P A
authors.initials: PA
authors.lastname: Mourão
authors.forename: C
authors.initials: C
authors.lastname: Boisson-Vidal
authors.forename: J
authors.initials: J
authors.lastname: Tapon-Bretaudière
authors.forename: B
authors.initials: B
authors.lastname: Drouet
authors.forename: A
authors.initials: A
authors.lastname: Bros
authors.forename: A
authors.initials: A
authors.lastname: Fischer
mesh_terms: D000818:Animals; D000925:Anticoagulants / Q000737:chemistry / Q000494:pharmacology; D001780:Blood Coagulation Tests; D002809:Chondroitin Sulfates / Q000737:chemistry / Q000494:pharmacology*; D004447:Echinodermata / Q000737:chemistry*; D005643:Fucose / Q000032:analysis / Q000737:chemistry; D006490:Hemostatics / Q000037:antagonists & inhibitors; D006801:Humans; D020128:Inhibitory Concentration 50; D007700:Kinetics; D015394:Molecular Structure; D012616:Sea Cucumbers / Q000737:chemistry; D013917:Thrombin / Q000037:antagonists & inhibitors*
chemical_list: D000925:Anticoagulants; D006490:Hemostatics; D005643:Fucose; D002809:Chondroitin Sulfates; D013917:Thrombin