Protective effect of phenolic compounds isolated from the hooks and stems of Uncaria sinensis on glutamate-induced neuronal death.
We isolated the phenolic compounds epicatechin, catechin, procyanidin B-1, procyanidin B-2, hyperin and caffeic acid from the hooks and stems of Uncaria sinensis (HSUS), and studied their protective effects against glutamate-induced neuronal death in cultured rat cerebellar granule cells. Cell viability evaluated by MTT assay was significantly increased by application of epicatechin (100-300 microM), catechin (300 microM), procyanidin B-1 (30-300 microM) and procyanidin B-2 (100-300 microM) compared with exposure to glutamate only. 45Ca2+ influx into cells induced by glutamate was also significantly inhibited by administration ofepicatechin (300 microM), catechin (300 microM), procyanidin B-1 (100-300 microM) and procyanidin B-2 (100-300 microM). These results suggest that epicatechin, catechin, procyanidin B-1 and procyanidin B-2 are the active components of HSUS that protect against glutamate-induced neuronal death in cultured cerebellar granule cells by inhibition of Ca2+ influx.
Shimada
Y
Y
Department of'Japanese Oriental Medicine, Faculty of Medicine, Toyama Medical and Pharmaceutical University.
Goto
H
H
Kogure
T
T
Shibahara
N
N
Sakakibara
I
I
Sasaki
H
H
Terasawa
K
K
D000818:Animals; D044946:Biflavonoids; D002109:Caffeic Acids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism; D002392:Catechin / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology; D004305:Dose-Response Relationship, Drug; D018698:Glutamic Acid / Q000009:adverse effects* / Q000494:pharmacology; D015394:Molecular Structure; D009474:Neurons / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D010936:Plant Extracts; D018547:Plant Stems; D010946:Plants, Medicinal / Q000737:chemistry*; D044945:Proanthocyanidins; D011794:Quercetin / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D019666:Rubiaceae / Q000737:chemistry*
D044946:Biflavonoids; D002109:Caffeic Acids; D010636:Phenols; D010936:Plant Extracts; D044945:Proanthocyanidins; C479578:procyanidin B; D018698:Glutamic Acid; C017674:procyanidin; C021304:hyperoside; D002392:Catechin; D011794:Quercetin; D002118:Calcium; C040048:caffeic acid