Structure of the exopolysaccharide produced by Streptococcus thermophilus S3.
The exopolysaccharide of Streptococcus thermophilus S3, produced in skimmed milk, is composed of D-galactose and L-rhamnose in a molar ratio of 2:1. The polysaccharide contains 0.4 equiv of O-acetyl groups per repeating unit. Linkage analysis and 1D/2D NMR (1H and 13C) studies on native and O-deacetylated EPS together with nanoES-CID tandem mass spectrometry studies on oligosaccharides generated by a periodate oxidation protocol, show the polysaccharide to have the following structure: [structure: see text].
Faber
E J
EJ
Department of Bio-Organic Chemistry, Bijvoet Center, Utrecht University, The Netherlands.
van den Haak
M J
MJ
Kamerling
J P
JP
Vliegenthart
J F
JF
D000818:Animals; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008892:Milk / Q000382:microbiology*; D008969:Molecular Sequence Data; D015394:Molecular Structure; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000302:isolation & purification; D021241:Spectrometry, Mass, Electrospray Ionization; D013291:Streptococcus / Q000378:metabolism*
D011135:Polysaccharides, Bacterial; C098632:exopolysaccharide, Streptococcus