Biochemical characterisation of a hydrophobic ligand binding protein from the tapeworm Hymenolepis diminuta.
The cestode Hymenolepis diminuta contains an abundant, cytoplasmic, hydrophobic ligand, binding protein (H-HLBP). Studies with polarity sensitive probes suggest a single hydrophobic binding site, the results also indicate that the single tryptophan in the molecule (Trp41) is involved in ligand binding. Of the possible physiological ligands tested, only haematin and retinoids (retinol and retinoic acid) show appreciable binding in addition to fatty acids. H-HLBP also binds a range of anthelmintics, again with K(D) values in the nM range. The interaction of anthelmintics with hydrophobic binding proteins may be important in determining drug specificity and site of action and could have a role in the development of drug resistance.
Saghir
N
N
CRC Beatson Laboratories, Garscube Estate, Switchback Road, G61 1BD, Glasgow, UK.
Conde
P J
PJ
Brophy
P M
PM
Barrett
J
J
D000818:Animals; D001667:Binding, Competitive; D002352:Carrier Proteins / Q000378:metabolism*; D003619:Dansyl Compounds / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D005456:Fluorescent Dyes / Q000378:metabolism; D015801:Helminth Proteins; D006427:Hemin / Q000378:metabolism; D006926:Hymenolepis / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D011487:Protein Conformation; D051381:Rats; D017208:Rats, Wistar; D013050:Spectrometry, Fluorescence; D014212:Tretinoin / Q000378:metabolism
D002352:Carrier Proteins; D003619:Dansyl Compounds; D005227:Fatty Acids; D005456:Fluorescent Dyes; D015801:Helminth Proteins; C419583:hydrophobic ligand binding protein, Hymenolepis diminuta; D014212:Tretinoin; C054400:11-(dansylamino)undecanoic acid; D006427:Hemin