[Cloning, sequencing and chromosome location of Sry gene of Muntjak munticus vaginalis by DOP-PCR and microdissection].
We isolated 1, Y1, Y2 chromosomes of the male M.m vaginalis by microdissection and amplified the DNA copies by DOP-PCR. Using the DOP-PCR products of 1, Y1, Y2 chromosomes of M.m vaginalis as template respectively and the primers designed within human SRY HMG box, the Sry gene of the male M.m vaginalis was amplified, and it was cloned and sequenced. It is proved that Y2 is the real sex chromosome in the male M.m vaginalis at molecular level for the first time, and Sry was localized on Y2 chromosome.
Zhang
Y
Y
Department of Biology, Medical College, Southweast University, Nanjing 210009, China.
Shan
X N
XN
Lu
X X
XX
Liu
N S
NS
Yu
D W
DW
D000818:Animals; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000235:genetics*; D004210:Dissection; D008297:Male; D018668:Muntjacs / Q000235:genetics*; D009687:Nuclear Proteins; D016133:Polymerase Chain Reaction; D051876:Sex-Determining Region Y Protein; D014157:Transcription Factors; D014998:Y Chromosome
D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D051876:Sex-Determining Region Y Protein; D014157:Transcription Factors