title: [Human angiogenin: expression, purification, biological assay].
abstract: Angiogenin cDNA was obtained by RT-PCR, and cloned into the fusion expression vector pRSETB. The recombinant Angiogenin protein was fused with His6 at its N-terminal and expressed as inclusion body. The expression level was about 10% of the total bacteria protein. After dissolved in 8 mol/L urea, the recombinant protein was purified by Ni2(+)-NTA chelating resin, according to the high affinity of His6 with Ni2+. The biological assay indicated that purified rhANG could induced the new blood vessel formation of CAM and degraded tRNA in vitro.
authors.affiliation: Fourth Military Medical University Biotechnology Center, Xi'an, China. bio707@fmmu.edu.cn
authors.forename: H
authors.initials: H
authors.lastname: Yang
authors.forename: Y Q
authors.initials: YQ
authors.lastname: Zhang
authors.forename: Z
authors.initials: Z
authors.lastname: Yan
authors.forename: W
authors.initials: W
authors.lastname: Han
authors.forename: L B
authors.initials: LB
authors.lastname: Yao
authors.forename: C Z
authors.initials: CZ
authors.lastname: Su
mesh_terms: D043925:Angiogenesis Inducing Agents / Q000096:biosynthesis*; D000818:Animals; D002642:Chick Embryo; D002846:Chromatography, Affinity; D006801:Humans; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis*; D012259:Ribonuclease, Pancreatic / Q000096:biosynthesis* / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D043925:Angiogenesis Inducing Agents; D011993:Recombinant Fusion Proteins; C046366:angiogenin; D012259:Ribonuclease, Pancreatic