Hydrodynamics-based gene delivery.
The need for a safe and efficient method for gene delivery has stimulated the recent emergence of vectorless methods (e.g., naked DNA) as promising alternatives to the available viral- and non-viral-based systems. Among these methods, hydrodynamics-based gene delivery through systemic injection of DNA offers a convenient, efficient and powerful means for high-level gene expression in mice. This review deals with the background and progress made so far with this new gene delivery method, the potential mechanism underlying the hydrodynamics-based DNA transfer into cells and its potential applications towards gene function analysis, protein expression and gene therapy in whole animals.
Liu
D
D
Department of Pharmaceutical Sciences, University of Pittsburgh School of Pharmacy, Pittsburgh, PA 15261, USA. dliu@vms.cis.pitt.edu
Knapp
J E
JE
D000818:Animals; D004247:DNA / Q000008:administration & dosage* / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation; D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D005822:Genetic Vectors / Q000378:metabolism; D022781:Hepatocytes / Q000502:physiology; D006801:Humans
D004247:DNA