Determination of essential amino acids involved in the CD4-independent tropism of the X4 human immunodeficiency virus type 1 m7NDK isolate: role of potential N glycosylations in the C2 and V3 regions of gp120.
Seven mutations in the C2, V3, and C3 regions of gp120 are implicated in the tropism of the first CD4-independent human immunodeficiency virus type 1 isolate, m7NDK. Site-directed mutagenesis revealed that three amino acids are essential to maintain this tropism, one in the C2 region and two in the V3 loop. Two mutations implied N glycosylation modifications.
Dumonceaux
J
J
INSERM Unite 380, Laboratoire de Génétique et Pathologie Expérimentales, Institut Cochin de Génétique Moléculaire, 75014 Paris, France.
Goujon
C
C
Joliot
V
V
Briand
P
P
Hazan
U
U
D000595:Amino Acid Sequence; D000601:Amino Acids, Essential / Q000032:analysis; D015704:CD4 Antigens / Q000502:physiology; D006031:Glycosylation; D015699:HIV Envelope Protein gp120 / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D015497:HIV-1 / Q000235:genetics* / Q000276:immunology; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation
D000601:Amino Acids, Essential; D015704:CD4 Antigens; D015699:HIV Envelope Protein gp120