title: [Purification of recombinant erythropoietin by reversed-phase high performance liquid chromatography (RP-HPLC)].
abstract: Recombinant erythropoietin is a glycoprotein which is strongly hydrophobic. We purified recombinant erythropoietin by RP-HPLC. The results showed that 30 nm Spherisorb C4 reversed-phase column and acetonitrile-TFA mobile phase with gradient elution, could readily isolate recombinant erythropoietin from crude samples with high speed and efficiency. Erythropoietin could be eluted at the concentration of about 50% acetonitrile with appropriate sample purity and elution gradient. When RP-HPLC is used as a final step of purification, the purity of sample should be at least 60%. The purity of the product tested by SDS-PAGE was nearly 100%. And the specific activity of the product was about 1.96 x 10(3) IU/g protein.
authors.affiliation: China Medical University, Shenyang, 110001.
authors.forename: Z
authors.initials: Z
authors.lastname: Zou
authors.forename: K
authors.initials: K
authors.lastname: Sun
authors.forename: D
authors.initials: D
authors.lastname: Lou
authors.forename: M
authors.initials: M
authors.lastname: Hu
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004921:Erythropoietin / Q000302:isolation & purification*; D011994:Recombinant Proteins
chemical_list: D011994:Recombinant Proteins; D004921:Erythropoietin