Characterization of a three-component vanillate O-demethylase from Moorella thermoacetica.
The Moorella thermoacetica aromatic O-demethylase was characterized as an inducible three-component system with similarity to the methanogenic methanol, methylamine, and methanethiol methyltransferases and to the O-demethylase system from Acetobacterium dehalogenans. MtvB catalyzes methyl transfer from a phenylmethylether to the cobalt center of MtvC, a corrinoid protein. MtvA catalyzes transmethylation from MtvC to tetrahydrofolate, forming methyltetrahydrofolate. Cobalamin can substitute for MtvC.
Naidu
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Department of Biochemistry, Beadle Center, University of Nebraska-Lincoln, Lincoln, NE 68588-0664, USA.
Ragsdale
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D000595:Amino Acid Sequence; D000693:Anaerobiosis; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D003013:Clostridium / Q000201:enzymology* / Q000254:growth & development; D003996:Dicamba / Q000378:metabolism; D016987:Gram-Positive Rods / Q000201:enzymology / Q000254:growth & development; D006879:Hydroxocobalamin / Q000378:metabolism; D007700:Kinetics; D008745:Methylation; D008780:Methyltransferases / Q000378:metabolism; D008969:Molecular Sequence Data; D010090:Oxidoreductases, O-Demethylating / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification* / Q000378:metabolism; D013763:Tetrahydrofolates / Q000378:metabolism; D014641:Vanillic Acid / Q000378:metabolism*; D014805:Vitamin B 12 / Q000031:analogs & derivatives* / Q000378:metabolism
D001426:Bacterial Proteins; D013763:Tetrahydrofolates; C030371:5,6,7,8-tetrahydrofolic acid; C019476:mecobalamin; D010090:Oxidoreductases, O-Demethylating; C023284:vanillate O-demethylase; D008780:Methyltransferases; D014641:Vanillic Acid; D014805:Vitamin B 12; D006879:Hydroxocobalamin; D003996:Dicamba