Analysis of clonal relationship using single-cell polymerase chain reaction in a patient with concomitant mantle cell lymphoma and multiple myeloma.
We report a case of concomitant mantle cell lymphoma (MCL) and multiple myeloma (MM) in which we investigated the possibility of a clonal relationship. A 76-year-old man was diagnosed with MCL [immunoglobulin (Ig)M,D-kappa; stage IVB] and MM (IgG-kappa; stage I). Ig heavy chain (IgH) gene complementarity-determining region 3 in DNA from both the MCL tumor and from single MM cells from bone marrow smears was amplified to investigate whether there was a clonal relationship between MCL and MM. Sequence analysis revealed no clonal relationship between MCL and MM in our patient.
Yamaguchi
M
M
Second Department of Internal Medicine, Mie University School of Medicine, Tsu, Japan. waniwani@clin.medic.mie-u.ac.jp
Ohno
T
T
Miyata
E
E
Toyoda
H
H
Nishii
K
K
Masuya
M
M
Kita
K
K
Shiku
H
H
D000368:Aged; D001853:Bone Marrow / Q000473:pathology; D002880:Chromosomes, Human, Pair 11 / Q000235:genetics / Q000648:ultrastructure; D002883:Chromosomes, Human, Pair 14 / Q000235:genetics / Q000648:ultrastructure; D002999:Clone Cells / Q000737:chemistry / Q000473:pathology; D004273:DNA, Neoplasm / Q000032:analysis; D005803:Genes, Immunoglobulin; D006801:Humans; D007078:Ileal Neoplasms / Q000235:genetics / Q000473:pathology*; D007080:Ileocecal Valve / Q000473:pathology*; D007074:Immunoglobulin G / Q000235:genetics; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D007145:Immunoglobulin kappa-Chains / Q000235:genetics; D020522:Lymphoma, Mantle-Cell / Q000235:genetics / Q000473:pathology*; D008297:Male; D009101:Multiple Myeloma / Q000235:genetics / Q000473:pathology*; D009194:Myeloma Proteins / Q000235:genetics; D009363:Neoplasm Proteins / Q000235:genetics; D009378:Neoplasms, Multiple Primary / Q000235:genetics / Q000473:pathology*; D014411:Neoplastic Stem Cells / Q000737:chemistry / Q000473:pathology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D014178:Translocation, Genetic
D004273:DNA, Neoplasm; D007074:Immunoglobulin G; D007143:Immunoglobulin Heavy Chains; D007145:Immunoglobulin kappa-Chains; D009194:Myeloma Proteins; D009363:Neoplasm Proteins