Micellar electrokinetic capillary chromatography quantification of cytosine arabinoside and its metabolite, uracil arabinoside, in human serum.
Cytosine arabinoside (Ara-C) is widely used to induce remission in adult granulocytic leukemia. High doses can be infused in refractory leukemia or in relapse. After injection, Ara-C is quickly metabolized to uracil arabinoside (Ara-U), the main inactive metabolite. We here described a micellar electrokinetic capillary chromatography (MECC) method to simultaneously determine Ara-C/Ara-U in human serum using 6-O-methylguanine as an internal standard. The assay was linear from 6.25 to 200 microg/ml with a quantification limit between 3 and 6 microg/ml. The analytical precision was satisfactory between 2 and 4.3% (within-run) and 3.7 and 7.3% (between-runs). This assay was applied to the analysis of serum from acute granulocytic leukemia patient treated by high doses cytarabine (3 g/m2 body surface).
Houzé
P
P
Laboratoire de Biochmie A, H pital Saint Louis, Paris, France.
Deschamps
F
F
Dombret
H
H
Bousquet
B
B
Gourmel
B
B
D000964:Antimetabolites, Antineoplastic / Q000097:blood* / Q000627:therapeutic use; D001086:Arabinofuranosyluracil / Q000097:blood*; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D003561:Cytarabine / Q000097:blood* / Q000302:isolation & purification / Q000627:therapeutic use; D006801:Humans; D007951:Leukemia, Myeloid / Q000188:drug therapy; D009994:Osmolar Concentration; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013696:Temperature
D000964:Antimetabolites, Antineoplastic; D003561:Cytarabine; D001086:Arabinofuranosyluracil