title: A novel technique that measures peptide secretion on a millisecond timescale reveals rapid changes in release.
abstract: Neuropeptides are ubiquitous transmitters that have been implicated in a wide variety of physiological and pathological conditions, and it is important to understand the processes that control their secretion. We have developed a technique that measures neuropeptide secretion with high temporal resolution. This method involves placing an electrophysiological "tag" in a neuropeptide prohormone. The tagged prohormone is subsequently expressed together with an ionotropic receptor that binds the tag. Because the neuropeptide of interest and the tag enter the same population of dense core granules, neuropeptide secretion gives rise to fast, synaptic-like currents. Using this method, we show that peptide secretion can be modulated on a millisecond time scale. This technique could be readily adapted to measure the secretion of any neuropeptide.
authors.affiliation: Department of Pharmacology, University College London, Gower Street, WC1E 6BT, London, United Kingdom. m.whim@ucl.ac.uk
authors.forename: M D
authors.initials: MD
authors.lastname: Whim
authors.forename: G W
authors.initials: GW
authors.lastname: Moss
mesh_terms: D000818:Animals; D015220:Calcium Channels / Q000187:drug effects / Q000378:metabolism; D004558:Electric Stimulation; D019835:FMRFamide / Q000235:genetics* / Q000378:metabolism; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D009478:Neuropeptide Y / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D009479:Neuropeptides / Q000378:metabolism*; D009482:Neurophysiology / Q000379:methods*; D009489:Neurosecretion / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D018010:Receptors, Invertebrate Peptide / Q000187:drug effects / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology; D013569:Synapses / Q000187:drug effects / Q000378:metabolism*; D009435:Synaptic Transmission / Q000502:physiology*; D013997:Time Factors
chemical_list: D015220:Calcium Channels; C055209:FMRFamide receptor; D009478:Neuropeptide Y; D009479:Neuropeptides; D018010:Receptors, Invertebrate Peptide; D019835:FMRFamide