Isolation and symbiotic characterization of aromatic amino acid auxotrophs of Sinorhizobium meliloti.
Ten aromatic amino acid auxotrophs of Sinorhizobium meliloti (previously called Rhizobium meliloti) Rmd201 were generated by random mutagenesis with transposon Tn5 and their symbiotic properties were studied. Normal symbiotic activity, as indicated by morphological features, was observed in the tryptophan synthase mutants and the lone tyrosine mutant. The trpE and aro mutants fixed trace amounts of nitrogen whereas the phe mutant was completely ineffective in nitrogen fixation. Histology of the nodules induced by trpE and aro mutants exhibited striking similarities. Each of these nodules contained an extended infection zone and a poorly developed nitrogen fixation zone. Transmission electron microscopic studies revealed that the bacteroids in the extended infection zone of these nodules did not show maturation tendency. A leaky mutant, which has a mutation in trpC, trpD, or trpF gene, was partially effective in nitrogen fixation. The histology of the nodules induced by this strain was like that of the nodules induced by the parental strain but the inoculated plants were stunted. These studies demonstrated the involvement of anthranilic acid and at least one more intermediate of tryptophan biosynthetic pathway in bacteroidal maturation and nitrogen fixation in S. meliloti. The alfalfa plant host seems to provide tryptophan and tyrosine but not phenylalanine to bacteroids in nodules.
Prasad
C K
CK
Department of Biosciences and Biotechnology, University of Roorkee, Roorkee 247 667, India.
Vineetha
K E
KE
Hassani
R
R
Gupta
R
R
Randhawa
G S
GS
D000596:Amino Acids / Q000378:metabolism*; D004251:DNA Transposable Elements; D000455:Medicago sativa / Q000382:microbiology*; D016296:Mutagenesis; D016962:Sinorhizobium meliloti / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism / Q000502:physiology; D013559:Symbiosis
D000596:Amino Acids; D004251:DNA Transposable Elements