title: Molecular cloning and analysis of the 5'-flanking region of the human MYPT1 gene.
abstract: We have cloned and sequenced a 5 kb genomic fragment in the 5'-flanking region of the human myosin phosphatase target subunit 1. The transcription initiation site (+1) was 268 bp upstream from the translation start site. In this promoter there are no canonical TATA or CAAT box elements but there is a high GC-rich sequence. Basal promoter activity was due to the GC-rich region that contained one Sp1 transcription factor binding site, thus demonstrating that the MYPT1 gene is a housekeeping gene. Luciferase reporter assays showed the presence of two regions for positive elements and one for a negative element.
authors.affiliation: First Department of Internal Medicine, University School of Medicine, Tsu, Mie, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Machida
authors.forename: M
authors.initials: M
authors.lastname: Ito
authors.forename: R
authors.initials: R
authors.lastname: Okamoto
authors.forename: K
authors.initials: K
authors.lastname: Shiraki
authors.forename: N
authors.initials: N
authors.lastname: Isaka
authors.forename: D J
authors.initials: DJ
authors.lastname: Hartshorne
authors.forename: T
authors.initials: T
authors.lastname: Nakano
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D008156:Luciferases / Q000235:genetics; D008969:Molecular Sequence Data; D043263:Myosin-Light-Chain Phosphatase; D010749:Phosphoprotein Phosphatases / Q000737:chemistry / Q000235:genetics*; D011401:Promoter Regions, Genetic; D021122:Protein Subunits; D016329:Sp1 Transcription Factor / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D017931:DNA Primers; D021122:Protein Subunits; D016329:Sp1 Transcription Factor; D004247:DNA; D008156:Luciferases; D010749:Phosphoprotein Phosphatases; D043263:Myosin-Light-Chain Phosphatase