title: Differentiation between reactive gliosis and diffuse astrocytoma by in situ hybridization.
abstract: The authors examined the use of chromosomal analysis by in situ hybridization to differentiate between nonneoplastic reactive gliosis and astrocytomas in cases in which routine histology was inconclusive. Numerical chromosomal aberrations were found in 80% of low-grade astrocytoma specimens and in none of the reactive gliosis specimens. Aneusomic tumor cells were detected in four of 13 stereotactic samples with an initially inconclusive tissue diagnosis, three of which were later diagnosed as astrocytoma. The in situ hybridization procedure may have additional value in the differential diagnosis of reactive gliosis versus low-grade astrocytoma.
authors.affiliation: Research Institute of Growth and Development (GROW), Department of Neurology, University Hospital Maastricht, the Netherlands. PWES@SNEU.AZM.NL
authors.forename: P H
authors.initials: PH
authors.lastname: Wessels
authors.forename: A H
authors.initials: AH
authors.lastname: Hopman
authors.forename: M I
authors.initials: MI
authors.lastname: Ummelen
authors.forename: B
authors.initials: B
authors.lastname: Krijne-Kubat
authors.forename: F C
authors.initials: FC
authors.lastname: Ramaekers
authors.forename: A
authors.initials: A
authors.lastname: Twijnstra
mesh_terms: D000328:Adult; D000368:Aged; D001254:Astrocytoma / Q000235:genetics / Q000473:pathology*; D001932:Brain Neoplasms / Q000235:genetics / Q000473:pathology*; D002878:Chromosomes, Human, Pair 1; D002879:Chromosomes, Human, Pair 10; D002897:Chromosomes, Human, Pair 7; D005911:Gliosis / Q000235:genetics / Q000473:pathology*; D006801:Humans; D017403:In Situ Hybridization; D008875:Middle Aged