Model systems: Studying molecular recognition using bacterial n-hybrid systems.
Since the first description of the yeast two-hybrid system, related genetic assays for protein-protein interactions have become popular and powerful tools for structure-function analysis on the scale of individual proteins or whole proteomes. After a somewhat surprising lag, similar systems have recently been described for use in bacterial hosts. n-hybrid modifications of the original yeast system have been used to examine interactions with DNA, RNA and small molecules, and other modifications have improved throughput for genomic applications. Bacterial n-hybrid systems are being designed for a similar array of uses. Will the bacterial systems be as popular as the yeast n-hybrid systems? Only time will tell.
Hu
J C
JC
Dept of Biochemistry and Biophysics, Texas A&M University, College Station, TX 77843-2128, USA. jimhu@tamu.edu
D001419:Bacteria / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D016680:Genome, Bacterial; D020798:Two-Hybrid System Techniques
D001426:Bacterial Proteins