Isolation of a novel candidate oncogene within a frequently amplified region at 3q26 in ovarian cancer.
Amplification of 3q25-q26 was one of the most frequent chromosomal alterations in human ovarian carcinoma. A chromosome microdissection-hybrid selection method was applied to isolate transcribed sequences from a primary ovarian cancer containing high-copy-number amplification of 3q26 using 3q26 band-specific DNAs generated by chromosome microdissection. Using this method, we have isolated a novel candidate oncogene eIF-5A2 (eukaryotic initiation factor 5A2). eIF-5A2 shares 82% identity of amino acid sequence with eIF-5A including the minimum domain needed for eIF-5A maturation by hypusine modification at lysine-50 residue. Amplification and overexpression of eIF-5A2 was frequently detected in primary ovarian cancers and ovarian cancer cell lines. The proliferation-related function of eIF-5A supports that eIF-5A2 is a candidate oncogene related to the development of ovarian cancer.
Guan
X Y
XY
Department of Clinical Oncology, Queen Mary Hospital, The University of Hong Kong. xyguan@hkucc.hku.hk
Sham
J S
JS
Tang
T C
TC
Fang
Y
Y
Huo
K K
KK
Yang
J M
JM
D000595:Amino Acid Sequence; D000818:Animals; D002645:Chickens; D002869:Chromosome Aberrations; D002874:Chromosome Mapping; D002893:Chromosomes, Human, Pair 3; D004273:DNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D005784:Gene Amplification; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010051:Ovarian Neoplasms / Q000235:genetics*; D010448:Peptide Initiation Factors / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
D004273:DNA, Neoplasm; D010448:Peptide Initiation Factors; C425967:eIF-5A2