title: Immunostimulating effect of pule (Alstonia scholaris L. R.Br., Apocynaceae) bark extracts.
abstract: The immunostimulating effect of "Pule" (Alstonia scholaris (L.) R.Br., Apocynaceae) bark extracts was studied in BALB/c mouse. The extracts were administered orally, once a day for 7 consecutive days. The results showed that at the same doses (50, 100 and 200 mg/kg b.w.) the aqueous extract had higher phagocytic index (1.39-1.79) than the ethanolic extracts (0.81-0.93) in normal mice. The aqueous extract at 50 mg/kg b.w. also enhanced phagocytic activity of immunosuppressed mice significantly (P < 0.01). At 50 and 100 mg/kg b.w. the extract prevents the decrease of immune system induced by prednisone. The aqueous extract at 100 mg/kg b.w. increased lytic activity of peritoneal exudate cells against Escherichia coli significantly (P < 0.05). At the doses of 50 and 100 mg/kg b.w. the aqueous extract had no effect on primary antibody level. The aqueous extract at 50 mg/kg b.w. induced the cellular immune response while at 100 mg/kg b.w. inhibited the delayed type of hypersensitivity reaction.
authors.affiliation: Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Institut Teknologi Bandung, Ganesa, Indonesia. immaiwo@yahoo.com
authors.forename: M I
authors.initials: MI
authors.lastname: Iwo
authors.forename: A A
authors.initials: AA
authors.lastname: Soemardji
authors.forename: D S
authors.initials: DS
authors.lastname: Retnoningrum
authors.lastname: Sukrasno
authors.forename: U M
authors.initials: UM
authors.lastname: U
mesh_terms: D000276:Adjuvants, Immunologic / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D000917:Antibody Formation / Q000187:drug effects; D001433:Bacteriolysis / Q000187:drug effects*; D004353:Drug Evaluation, Preclinical; D004926:Escherichia coli; D000431:Ethanol; D005260:Female; D006386:Hemagglutination Tests; D006968:Hypersensitivity, Delayed / Q000276:immunology; D007111:Immunity, Cellular / Q000187:drug effects*; D007166:Immunosuppressive Agents / Q000037:antagonists & inhibitors; D007214:Indonesia; D008099:Liver / Q000187:drug effects; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D012157:Mononuclear Phagocyte System / Q000187:drug effects / Q000502:physiology; D009113:Muramidase / Q000494:pharmacology; D009929:Organ Size / Q000187:drug effects; D010529:Peritoneal Cavity / Q000166:cytology; D010587:Phagocytosis / Q000187:drug effects*; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology; D018547:Plant Stems / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011241:Prednisone / Q000037:antagonists & inhibitors; D013154:Spleen / Q000187:drug effects; D014197:Trees / Q000737:chemistry*; D014867:Water
chemical_list: D000276:Adjuvants, Immunologic; D007166:Immunosuppressive Agents; D010936:Plant Extracts; D014867:Water; D000431:Ethanol; D009113:Muramidase; D011241:Prednisone