title: Hydrogen peroxide mediates the cell growth and transformation caused by the mitogenic oxidase Nox1.
abstract: Nox1, a homologue of gp91phox, the catalytic moiety of the superoxide (O(2)(-))-generating NADPH oxidase of phagocytes, causes increased O(2)(-) generation, increased mitotic rate, cell transformation, and tumorigenicity when expressed in NIH 3T3 fibroblasts. This study explores the role of reactive oxygen species (ROS) in regulating cell growth and transformation by Nox1. H(2)O(2) concentration increased approximately 10-fold in Nox1-expressing cells, compared with <2-fold increase in O(2)(-). When human catalase was expressed in Nox1-expressing cells, H(2)O(2) concentration decreased, and the cells reverted to a normal appearance, the growth rate normalized, and cells no longer produced tumors in athymic mice. A large number of genes, including many related to cell cycle, growth, and cancer (but unrelated to oxidative stress), were expressed in Nox1-expressing cells, and more than 60% of these returned to normal levels on coexpression of catalase. Thus, H(2)O(2) in low concentrations functions as an intracellular signal that triggers a genetic program related to cell growth.
authors.affiliation: Department of Biochemistry, Atlanta Veterans Affairs Medical Center and Winship Cancer Institute, Emory University School of Medicine, Atlanta, GA 30322, USA.
authors.forename: R S
authors.initials: RS
authors.lastname: Arnold
authors.forename: J
authors.initials: J
authors.lastname: Shi
authors.forename: E
authors.initials: E
authors.lastname: Murad
authors.forename: A M
authors.initials: AM
authors.lastname: Whalen
authors.forename: C Q
authors.initials: CQ
authors.lastname: Sun
authors.forename: R
authors.initials: R
authors.lastname: Polavarapu
authors.forename: S
authors.initials: S
authors.lastname: Parthasarathy
authors.forename: J A
authors.initials: JA
authors.lastname: Petros
authors.forename: J D
authors.initials: JD
authors.lastname: Lambeth
mesh_terms: D000818:Animals; D001483:Base Sequence; D002374:Catalase / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects* / Q000502:physiology; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D017931:DNA Primers; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D051379:Mice; D008819:Mice, Nude; D009247:NADH, NADPH Oxidoreductases / Q000502:physiology*; D000074624:NADPH Oxidase 1; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D006861:Hydrogen Peroxide; D002374:Catalase; D009247:NADH, NADPH Oxidoreductases; D000074624:NADPH Oxidase 1; C000614711:NOX1 protein, mouse