Expression of hMSH2 and hMLH1 proteins of the human DNA mismatch repair system in ameloblastoma.
The human DNA mismatch repair (hMMR) system plays an important role in reducing mutation and maintaining genomic stability. The MMR system in human cells is composed of at least six genes (hMSH2, hMLH1, hMSH3, hPMS1, hPMS2 and GTBP/hMSH6). In particular, hMSH2 and hMLH1 are expressed in human cells that are undergoing rapid renewal; their reduced expression has been reported in several tumors. We examined the protein expression pattern of hMSH2 and hMLH1 by immunohistochemistry in 25 ameloblastomas. All ameloblastomas expressed hMSH2 and hMLH1 proteins in the outer layer of epithelial cells. The localization of the staining was exclusively nuclear. These data suggest that the development and progression of these tumors do not depend on a defect in the hMMR system.
Castrilli
G
G
Department of Oncology, University of Chieti, Italy.
Piantelli
M
M
Artese
L
L
Perfetti
G
G
Rubini
C
C
Fioroni
M
M
Ranelletti
F O
FO
Piattelli
A
A
D000564:Ameloblastoma / Q000235:genetics* / Q000378:metabolism; D020137:Base Pair Mismatch; D004260:DNA Repair / Q000235:genetics*; D004268:DNA-Binding Proteins; D006801:Humans; D007150:Immunohistochemistry; D007573:Jaw Neoplasms / Q000235:genetics* / Q000378:metabolism; D018895:Microsatellite Repeats; D051718:MutS Homolog 2 Protein; D014176:Protein Biosynthesis; D011506:Proteins; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis*
D004268:DNA-Binding Proteins; D011506:Proteins; D011518:Proto-Oncogene Proteins; C497172:MSH2 protein, human; D051718:MutS Homolog 2 Protein