Inflammatory stimuli increase prostaglandin D synthase levels in cerebrospinal fluid of rats.
To examine the possibility that prostaglandin D synthase in CSF is involved in the brain responses to inflammation, we monitored the enzyme levels before and after administration of a bacterial endotoxin lipopolysaccharide (LPS) or proinflammatory cytokines. After an i.p. injection of LPS, the synthase levels began to rise within 1 day, peaked at 2 days and returned to the basal by 5 days after injection. The enzyme levels increased in a similar manner in response to tumor necrosis factor-alpha or interleukin (IL)-1 beta injected i.v. Centrally administered IL-1 beta had a similar effect. These findings support the hypothesis that PGD synthase in CSF participates in the brain responses to inflammation.
Ishizaka
M
M
Institute for Molecular and Cellular Regulation, Gunma University, Showa-machi, Maebashi, Gunma 371, Japan.
Ohe
Y
Y
Senbongi
T
T
Wakabayashi
K
K
Ishikawa
K
K
D000818:Animals; D007249:Inflammation / Q000139:chemically induced* / Q000201:enzymology*; D007274:Injections, Intraperitoneal; D007275:Injections, Intravenous; D007276:Injections, Intraventricular; D007375:Interleukin-1 / Q000008:administration & dosage; D019746:Intramolecular Oxidoreductases / Q000134:cerebrospinal fluid*; D054834:Lipocalins; D008070:Lipopolysaccharides / Q000008:administration & dosage; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D014409:Tumor Necrosis Factor-alpha / Q000008:administration & dosage
D007375:Interleukin-1; D054834:Lipocalins; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D019746:Intramolecular Oxidoreductases; C022466:prostaglandin R2 D-isomerase