title: DNA breakage induced by 1,2,4-benzenetriol: relative contributions of oxygen-derived active species and transition metal ions.
abstract: We report here the relative roles of metals and selected reactive oxygen species in DNA damage by the genotoxic benzene metabolite 1,2,4-benzenetriol, and the interactions of antioxidants in affording protection. 1,2,4-Benzenetriol induces scission in supercoiled phage DNA in neutral aqueous solution with an effective dose (ED(50)) of 6.7 microM for 50% cleavage of 2.05 microg/ml supercoiled PM2 DNA. In decreasing order of effectiveness: catalase (20 U/ml), formate (25 mM), superoxide dismutase (20 U/ml), and mannitol (50 mM) protected, from 85 to 28%. Evidently, H(2)O(2) is the dominant active species, with O(2)(*)(-) and *OH playing subordinate roles. Desferrioxamine or EDTA inhibited DNA breakage by 81-85%, despite accelerating 1,2,4-benzenetriol autoxidation. Consistent with this suggestion of a crucial role for metals, addition of cupric, cuprous, ferric, or ferrous ions enhanced DNA breakage, with copper being more active than iron. Combinations of scavengers protected more effectively than any single scavenger alone, with implications for antioxidants acting in concert in living cells. Synergistic combinations were superoxide dismutase with *OH scavengers, superoxide dismutase with desferrioxamine, and catalase with desferrioxamine. Antagonistic (preemptive) combinations were catalase with superoxide dismutase, desferrioxamine with *OH scavengers, and catalase with *OH scavengers. The most striking aspect of synergism was the extent to which metal chelation (desferrioxamine) acted synergistically with either catalase or superoxide dismutase to provide virtually complete protection. Concluding, 1,2,4-benzenetriol-induced DNA damage occurs mainly by site-specific, Fenton-type mechanisms, involving synergism between several reactive intermediates. Multiple antioxidant actions are needed for effective protection.
authors.affiliation: Bioenergetics Research Laboratory, Faculty of Applied Sciences, Simon Fraser University, Burnaby, BC, Canada.
authors.forename: A S
authors.initials: AS
authors.lastname: Li
authors.forename: B
authors.initials: B
authors.lastname: Bandy
authors.forename: S
authors.initials: S
authors.lastname: Tsang
authors.forename: A J
authors.initials: AJ
authors.lastname: Davison
mesh_terms: D002374:Catalase / Q000494:pharmacology; D002614:Chelating Agents / Q000494:pharmacology; D004249:DNA Damage; D004269:DNA, Bacterial / Q000187:drug effects / Q000378:metabolism; D004347:Drug Interactions; D005561:Formates / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000378:metabolism; D006873:Hydroquinones / Q000633:toxicity*; D008353:Mannitol / Q000494:pharmacology; D008670:Metals / Q000378:metabolism; D009153:Mutagens / Q000633:toxicity; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000494:pharmacology
chemical_list: D002614:Chelating Agents; D004269:DNA, Bacterial; D005561:Formates; D016166:Free Radical Scavengers; D006873:Hydroquinones; D008670:Metals; D009153:Mutagens; D017382:Reactive Oxygen Species; C030544:formic acid; C013038:hydroxyhydroquinone; D008353:Mannitol; D006861:Hydrogen Peroxide; D002374:Catalase; D013482:Superoxide Dismutase