title: Interactions of the COP9 signalosome with the E3 ubiquitin ligase SCFTIRI in mediating auxin response.
abstract: The COP9 signalosome is an evolutionary conserved multiprotein complex of unknown function that acts as a negative regulator of photomorphogenic seedling development in Arabidopsis. Here, we show that plants with reduced COP9 signalosome levels had decreased auxin response similar to loss-of-function mutants of the E3 ubiquitin ligase SCFTIR1. Furthermore, we found that the COP9 signalosome and SCFTIR1 interacted in vivo and that the COP9 signalosome was required for efficient degradation of PSIAA6, a candidate substrate of SCFTIR1. Thus, the COP9 signalosome may play an important role in mediating E3 ubiquitin ligase-mediated responses.
authors.forename: C
authors.initials: C
authors.lastname: Schwechheimer
authors.forename: G
authors.initials: G
authors.lastname: Serino
authors.forename: J
authors.initials: J
authors.lastname: Callis
authors.forename: W L
authors.initials: WL
authors.lastname: Crosby
authors.forename: S
authors.initials: S
authors.lastname: Lyapina
authors.forename: R J
authors.initials: RJ
authors.lastname: Deshaies
authors.forename: W M
authors.initials: WM
authors.lastname: Gray
authors.forename: M
authors.initials: M
authors.lastname: Estelle
authors.forename: X W
authors.initials: XW
authors.lastname: Deng
mesh_terms: D017360:Arabidopsis / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D001937:Brassica; D000075686:COP9 Signalosome Complex; D003624:Darkness; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017930:Genes, Reporter / Q000235:genetics; D007210:Indoleacetic Acids / Q000494:pharmacology*; D008025:Ligases / Q000235:genetics / Q000378:metabolism*; D046912:Multiprotein Complexes; D009154:Mutation / Q000235:genetics; D018532:Pisum sativum; D010447:Peptide Hydrolases; D010641:Phenotype; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000187:drug effects / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D030821:Plants, Genetically Modified; D011233:Precipitin Tests; D011485:Protein Binding; D014176:Protein Biosynthesis; D021122:Protein Subunits; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D016372:RNA, Antisense / Q000235:genetics; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D020798:Two-Hybrid System Techniques; D044767:Ubiquitin-Protein Ligases
chemical_list: D007210:Indoleacetic Acids; D046912:Multiprotein Complexes; D010940:Plant Proteins; D021122:Protein Subunits; D011506:Proteins; D016372:RNA, Antisense; D018749:RNA, Plant; D044767:Ubiquitin-Protein Ligases; D010447:Peptide Hydrolases; D000075686:COP9 Signalosome Complex; D008025:Ligases