title: Mechanical stimulation induces CTGF expression in rat osteocytes.
abstract: Connective tissue growth factor (CTGF), which is encoded by an immediate early gene and a member of the CCN family, has been shown to be expressed in osteoblasts, fibroblasts, and chondrocytes. Although CTGF is expressed in bone and cartilage tissues, we tested the hypothesis that CTGF is regulated in mechanotransduction. In the alveolar bone during experimental tooth movement, CTGF mRNA was expressed in osteoblasts and in osteocytes localized around the periodontal ligament under control conditions. Interestingly, 12 hrs after the start of experimental tooth movement, the expression of CTGF mRNA in osteocytes and osteoblasts became more intense around the periodontal ligament, and the intense expression of CTGF extended to osteocytes situated deep in alveolar bone matrix apart from periodontal ligament in both tension and compression sides. Our present findings indicate that CTGF could play a role in regulation of osteocyte function during the mechanical stimulation of bone.
authors.affiliation: Department of Orthodontics, Okayama University Dental School, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Yamashiro
authors.forename: T
authors.initials: T
authors.lastname: Fukunaga
authors.forename: N
authors.initials: N
authors.lastname: Kobashi
authors.forename: H
authors.initials: H
authors.lastname: Kamioka
authors.forename: T
authors.initials: T
authors.lastname: Nakanishi
authors.forename: M
authors.initials: M
authors.lastname: Takigawa
authors.forename: T
authors.initials: T
authors.lastname: Takano-Yamamoto
mesh_terms: D000539:Alveolar Process / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D055513:Connective Tissue Growth Factor; D003799:Dental Stress Analysis; D005786:Gene Expression Regulation; D006133:Growth Substances / Q000096:biosynthesis* / Q000235:genetics; D017874:Immediate-Early Proteins / Q000096:biosynthesis* / Q000235:genetics; D017403:In Situ Hybridization; D036341:Intercellular Signaling Peptides and Proteins; D008297:Male; D008934:Mitogens / Q000096:biosynthesis*; D010006:Osteoblasts / Q000378:metabolism; D010011:Osteocytes / Q000378:metabolism*; D010513:Periodontal Ligament / Q000502:physiology; D010812:Physical Stimulation; D015347:RNA Probes; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D014087:Tooth Movement Techniques
chemical_list: C526316:CCN2 protein, rat; D006133:Growth Substances; D017874:Immediate-Early Proteins; D036341:Intercellular Signaling Peptides and Proteins; D008934:Mitogens; D015347:RNA Probes; D012333:RNA, Messenger; D055513:Connective Tissue Growth Factor