title: [An animal experiment and clinical investigation on the protective effect of selenium on the microcirculation induced by free radical damaged RBCs].
abstract: Fluorescent labeling image analysis was used to evaluate the changes in cerebral arteriole and veinlet diameters (D), circulation velocities (FV) and permeability (VP) in rats; while in clinics, a laser-doppler device was used for assessing changes of skins and muscles microcirculation. The results show that in control rats, equal volume perfusion of free radical damaged RBCs resulted in decreases of D and FV significantly but VP was increased, whereas in the case when free radical damaged RBCs were perfused together with selenium, no disturbances in the D and VP were observed with FV even improved. In the human control group, either average skin microcirculative perfusions (ASMP) at 25 degrees C or maximal skin microcirculative perfusions (MSMP) at 42 degrees C were evidently decreased during myocardial ischemia/reperfusion period, while ASMP at 24th hour of post-surgery was kept reducing. At the same time, the changes in muscles microcirculative perfusions (MMP) tended to be similar to the skin, but overloaded than the pre-surgery levels at 24th hour of post-surgery period. In the selenium group before surgery, the ratio of MSMP to ASMP was obviously increased than the control group (3.95 in Se group vs 1.74 in control group, P < 0.05), but did not have significantly differences in ASMP, MSMP and MMP between the two groups during surgery period. RBC deformabilities were not changed. At 24th hour post-surgery, the ASMP were almost restored to pre-surgery levels. However, MMP were still lower than the pre-surgery levels.
  
  
  CONCLUSIONS
  (1) free radical damaged RBC perfusion leads to damage of microcirculation; (2) selenium is highly efficient in protecting microcirculation from free radical damaged RBC disturbance; and (3) Oral administration of selenium may improve pre-surgery maximal skin microcirculative perfusion and promote recovery of the worsened skin microcirculation in addition to prevent the occurrence of RBC deformability.
authors.affiliation: Hemorheology Department, Beijing Institute of Heart Lung and Blood Vessel Disease, Beijing 100029.
authors.forename: Y M
authors.initials: YM
authors.lastname: Huang
authors.forename: S
authors.initials: S
authors.lastname: Liu
authors.forename: Y X
authors.initials: YX
authors.lastname: Liu
authors.forename: D J
authors.initials: DJ
authors.lastname: Lin
authors.forename: C G
authors.initials: CG
authors.lastname: Duan
authors.forename: H W
authors.initials: HW
authors.lastname: Li
authors.forename: R J
authors.initials: RJ
authors.lastname: Xiu
authors.forename: J
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D002560:Cerebrovascular Circulation / Q000187:drug effects*; D002648:Child; D004907:Erythrocyte Deformability / Q000187:drug effects; D004910:Erythrocyte Membrane / Q000502:physiology*; D005260:Female; D005609:Free Radicals; D006343:Heart Septal Defects / Q000097:blood / Q000503:physiopathology / Q000601:surgery; D006801:Humans; D008297:Male; D008833:Microcirculation / Q000187:drug effects; D015428:Myocardial Reperfusion Injury / Q000503:physiopathology*; D011300:Preoperative Care; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012643:Selenium / Q000494:pharmacology* / Q000627:therapeutic use; D012867:Skin / Q000098:blood supply
chemical_list: D005609:Free Radicals; D012643:Selenium