title: Negative regulation of YY1 transcription factor on the dynamin I gene promoter.
abstract: Dynamin I is highly expressed in brain and plays a critical role in clathrin-mediated endocytosis and synaptic vesicle recycling. To elucidate the molecular mechanism by which expression of dynamin I is tissue-specifically regulated, we previously cloned and characterized the promoter of the mouse dynamin I gene and suggested that there is a negative regulatory element in this promoter region. In the present study, we showed that YY1 binds to this negative regulatory element located at -111 to -107 by using the EMSA and supershift analyses. Cotransfection experiment using an YY1 expression vector revealed that YY1 exerts a repressive role on the dynamin I gene promoter activity. These results demonstrate that transcription factor YY1 negatively regulates dynamin I expression via binding to the negative regulatory element.
authors.affiliation: Cell Biology Laboratory, Korea Research Institute of Bioscience and Biotechnology, Taejon, Yusung, 305-600, Korea.
authors.forename: J
authors.initials: J
authors.lastname: Yoo
authors.forename: M J
authors.initials: MJ
authors.lastname: Jeong
authors.forename: S S
authors.initials: SS
authors.lastname: Lee
authors.forename: K I
authors.initials: KI
authors.lastname: Lee
authors.forename: B M
authors.initials: BM
authors.lastname: Kwon
authors.forename: Y M
authors.initials: YM
authors.lastname: Park
authors.forename: M Y
authors.initials: MY
authors.lastname: Han
mesh_terms: D000818:Animals; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D001667:Binding, Competitive; D002460:Cell Line; D004247:DNA / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism*; D034284:Dynamin I; D034281:Dynamins; D050981:Erythroid-Specific DNA-Binding Factors; D020558:GTP Phosphohydrolases / Q000235:genetics*; D005786:Gene Expression Regulation; D051379:Mice; D011401:Promoter Regions, Genetic; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism*; D014162:Transfection; D050986:YY1 Transcription Factor
chemical_list: D004268:DNA-Binding Proteins; D050981:Erythroid-Specific DNA-Binding Factors; D014157:Transcription Factors; D050986:YY1 Transcription Factor; C494699:Yy1 protein, mouse; D004247:DNA; D034284:Dynamin I; D020558:GTP Phosphohydrolases; D034281:Dynamins