Expression pattern of the CsPK3 auxin-responsive protein kinase gene.
We have previously cloned a cDNA of a putative serine/threonine protein kinase gene named CsPK3 from cucumber, the mRNA level of which was up-regulated by auxin and down-regulated by light irradiation. To examine the CsPK3 gene expression in detail, we cloned a genomic DNA of CsPK3 gene and made transgenic tobacco (Nicotiana tabacum L. cv. Petit Havana SR1) plants containing the fused CsPK3 promoter-beta-glucuronidase gene. The beta-glucuronidase expression was detected in the shoot apex, vascular tissues, and the outermost layer of cortex. The histological distribution of CsPK3 mRNA in cucumber seedlings was supported by in situ hybridization, where the positive signals were observed in similar tissues as those observed by beta-glucuronidase staining. The responsiveness of the CsPK3 gene to auxin and light was also confirmed for beta-glucuronidase activity. The pattern of beta-glucuronidase staining changed during the development of the tobacco seedlings. The results of our experiment showed that CsPK3 was expressed in a wide variety of tissues and cells in which the developmental and growth controls by auxin are suggested.
Chono
M
M
Department of Applied Biological Chemistry, The University of Tokyo, Japan.
Suzuki
Y
Y
Nemoto
K
K
Yamane
H
H
Murofushi
N
N
Yamaguchi
I
I
D018553:Cucumis sativus / Q000201:enzymology* / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D018745:Genome, Plant; D007210:Indoleacetic Acids / Q000494:pharmacology*; D008027:Light; D010937:Plant Growth Regulators / Q000494:pharmacology*; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D011401:Promoter Regions, Genetic; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D017422:Sequence Analysis, DNA; D014018:Tissue Distribution; D014026:Nicotiana
D007210:Indoleacetic Acids; D010937:Plant Growth Regulators; C030737:indoleacetic acid; D017346:Protein Serine-Threonine Kinases