title: Internal standard high-performance liquid chromatography method for the determination of obidoxime in urine of organophosphate-poisoned patients.
abstract: Obidoxime is an antidote approved for reactivation of inhibited acetylcholinesterase in organophosphate poisoning. HPLC methods were described for its determination in blood or aqueous solutions but not for the determination in urine. Since data for renal obidoxime excretion ranged from 2.2 to 84% of administered dose in healthy volunteers depending on the route of administration and little is known about pharmacokinetics of obidoxime in severely intoxicated patients we developed an internal standard (HI 6) reversed-phase HPLC method for determining obidoxime in urine. The mobile phase consisted of methanol, the counter ion 1-heptane sulfonic acid and tetrabutylammonium phosphate, the stationary phase involved a 5 microm reversed-phase column (125x4 mm). Obidoxime was detected spectrophotometrically at 288 nm. The limit of quantification (LOQ) was 1 microM, the limit of detection (LOD) 0.5 microM. Linear calibration curves were obtained in a concentration range from 1 to 1000 microM. Intra- and inter-day precision C.V.s were below 4%. Accuracy was 95.9% in the LOQ range. Using this method, we were able to quantify obidoxime in urine of an organophosphate poisoned patient. Based on this data we calculated that 58% of the administered dose was excreted in the urine.
authors.affiliation: Institut fuer Pharmakologie und Toxikologie, Garching-Hochbrueck, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Grasshoff
authors.forename: H
authors.initials: H
authors.lastname: Thiermann
authors.forename: T
authors.initials: T
authors.lastname: Gillessen
authors.forename: T
authors.initials: T
authors.lastname: Zilker
authors.forename: L
authors.initials: L
authors.lastname: Szinicz
mesh_terms: D002801:Cholinesterase Reactivators / Q000493:pharmacokinetics / Q000652:urine*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D006801:Humans; D007306:Insecticides / Q000506:poisoning*; D008297:Male; D008875:Middle Aged; D009768:Obidoxime Chloride / Q000493:pharmacokinetics / Q000652:urine*; D010278:Parathion / Q000506:poisoning*; D011041:Poisoning / Q000188:drug therapy; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
chemical_list: D002801:Cholinesterase Reactivators; D007306:Insecticides; D009768:Obidoxime Chloride; D010278:Parathion