title: Comparisons of intraosseous graft healing between the doubled flexor tendon graft and the bone-patellar tendon-bone graft in anterior cruciate ligament reconstruction.
abstract: OBJECTIVE
  The purpose of this study was to compare intraosseous graft healing between the doubled flexor tendon (FT) graft and the bone-patellar tendon-bone (BPTB) graft in anterior cruciate ligament (ACL) reconstruction.
  
  
  METHODS
  Randomized trial.
  
  
  METHODS
  A biomechanical and histologic study was conducted with 24 adult beagle dogs. Bilateral ACL reconstructions were performed in each animal. Autogenous doubled FT and BPTB grafts were used for the left and right knees, respectively. Each end of the 2 grafts was tethered with a polyester suture to a screw post with a washer. The animals were then allowed unrestricted activities in their cages. Eight animals were killed at 3, 6, and 12 weeks, respectively.
  
  
  RESULTS
  Histologically, the FT graft was anchored to the tunnel wall with newly formed collagen fibers resembling Sharpey's fibers by 12 weeks. These fibers were more abundant in the anterior (ventral) gap than in the posterior (dorsal) gap. In the BPTB graft, the bone plug was anchored with newly formed bone at 3 weeks, although osteocytes in the plug trabeculae were necrotic for 12 weeks. Degeneration of the tendon-bone junction in the plug progressed at 6 weeks. Tensile testing showed that the weakest site was different not only between the 2 grafts but also between the observation periods. In the FT graft, the weakest site was the graft-wall interface at 3 weeks and the intraosseously grafted tendon at 6 weeks. In the BPTB graft, the weakest site was the graft-wall interface at 3 weeks and the proximal site in the bone plug at 6 weeks. The ultimate failure load of the FT graft was significantly inferior (45.8%) to that of the BPTB graft at 3 weeks (P =.021). At 6 weeks, the load of the FT graft was 85% that of the BPTB graft without a significant difference (P =.395).
  
  
  CONCLUSIONS
  As to the clinical relevance, the fixation device chosen for soft-tissue fixation appears to be more important than comparing it to the BPTB graft, although this has yet to be conclusively proven.
authors.affiliation: Department of Orthopaedic Surgery, Hokkaido University School of Medicine, Sapporo, Japan.
authors.forename: F
authors.initials: F
authors.lastname: Tomita
authors.forename: K
authors.initials: K
authors.lastname: Yasuda
authors.forename: S
authors.initials: S
authors.lastname: Mikami
authors.forename: T
authors.initials: T
authors.lastname: Sakai
authors.forename: S
authors.initials: S
authors.lastname: Yamazaki
authors.forename: H
authors.initials: H
authors.lastname: Tohyama
mesh_terms: D000818:Animals; D016118:Anterior Cruciate Ligament / Q000503:physiopathology / Q000601:surgery*; D000070598:Anterior Cruciate Ligament Injuries; D003094:Collagen / Q000032:analysis; D004285:Dogs; D004548:Elasticity; D007719:Knee Joint / Q000503:physiopathology* / Q000601:surgery*; D013536:Suture Techniques; D013710:Tendons / Q000473:pathology / Q000503:physiopathology / Q000637:transplantation*; D013718:Tensile Strength; D013977:Tibia / Q000473:pathology / Q000601:surgery*; D016896:Treatment Outcome; D016474:Weight-Bearing; D014945:Wound Healing
chemical_list: D003094:Collagen