title: Lipid phosphate phosphatase-1 dephosphorylates exogenous lysophosphatidate and thereby attenuates its effects on cell signalling.
abstract: The serum-derived phospholipid growth factor, lysophosphatidate (LPA), activates cells through a family of G-protein-coupled EDG receptors. The present article examines the role of lipid phosphate phosphatase-1 (LPP-1, or phosphatidate phosphate 2A) in regulating cell activation by LPA. Overexpressing LPP-1 approximately doubled the rate of dephosphorylation of exogenous LPA by Rat2 fibroblasts. The amount of LPA dephosphorylation was restricted to less than 10% of the total exogenous LPA. Over-expression of LPP-1 attenuated cell activation as indicated by diminished responses including cAMP, Ca2+, activation of phospholipase D and ERK, DNA synthesis and cell division. LPP-1 therefore provides a novel level of regulation for controlling cell signalling by exogenous LPA.
authors.affiliation: Department of Biochemistry, University of Alberta, Edmonton, Canada.
authors.forename: C
authors.initials: C
authors.lastname: Pilquil
authors.forename: I
authors.initials: I
authors.lastname: Singh
authors.forename: Q X
authors.initials: QX
authors.lastname: Zhang
authors.forename: Z C
authors.initials: ZC
authors.lastname: Ling
authors.forename: K
authors.initials: K
authors.lastname: Buri
authors.forename: L M
authors.initials: LM
authors.lastname: Stromberg
authors.forename: J
authors.initials: J
authors.lastname: Dewald
authors.forename: D N
authors.initials: DN
authors.lastname: Brindley
mesh_terms: D000818:Animals; D008246:Lysophospholipids / Q000378:metabolism*; D009687:Nuclear Proteins / Q000378:metabolism; D010711:Phosphatidate Phosphatase / Q000378:metabolism*; D051381:Rats; D011956:Receptors, Cell Surface / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled; D049368:Receptors, Lysophosphatidic Acid; D015398:Signal Transduction; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D008246:Lysophospholipids; D009687:Nuclear Proteins; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; D049368:Receptors, Lysophosphatidic Acid; D014157:Transcription Factors; C112948:lipid phosphate phosphatase; D010711:Phosphatidate Phosphatase