Distribution of somatostatin (SS) immunoreactivity using specific rabbit antibodies in preoptic-anterior hypothalamus (PO-AH) of female rat brain.
Distribution of somatostatin (SS) immunoreactivity was observed in the preoptic-anterior hypothalamus (PO-AH) of the female rat brain. Six specific rabbit antibodies A (SS-14), B (SS-28), C (SS-28 complete), D (SS 14 and SS-28 both), E (SS preprohormone) and F (GHRH) were used for immunostaining using Avidin-Biotin Complex (ABC) method (Hsu et al., 1981). Immunostaining was observed with all the six antibodies, in the serial sections passing through various bregma levels (-0.3 to -3.3 mm) of preoptic-anterior hypothalamic (PO-AH) region including median eminence (ME). In conclusion, the present study suggests that immunoreactive nerve terminals for both SS-14 and SS-28 are present in internal (IZ) and external zones (EZ) of ME. High intensity of SS-14 and SS-28 containing terminals in EZ suggests that both SS fractions are involved in regulating GH secretion in anterior pituitary. This is a first report on comparative distribution of immunoreactivities of four different fractions of SS, SS-preprohormone and GHRH in PO-AH.
Bhatnagar
M
M
Department of Zoology, University College of Science, M.L.S. University, 313001, Udaipur, India. mbhatnagar@yahoo.com
D000818:Animals; D005260:Female; D013007:Growth Hormone-Releasing Hormone / Q000378:metabolism; D007032:Hypothalamus, Anterior / Q000378:metabolism*; D007158:Immunologic Techniques; D008473:Median Eminence / Q000378:metabolism; D009411:Nerve Endings / Q000378:metabolism; D011301:Preoptic Area / Q000378:metabolism*; D011355:Prodrugs / Q000378:metabolism; D011817:Rabbits; D051381:Rats; D013004:Somatostatin / Q000378:metabolism*; D053561:Somatostatin-28; D013194:Staining and Labeling; D014018:Tissue Distribution
D011355:Prodrugs; D013004:Somatostatin; D053561:Somatostatin-28; D013007:Growth Hormone-Releasing Hormone