Insights into the physiological function of cellular prion protein.
Prions have been extensively studied since they represent a new class of infectious agents in which a protein, PrPsc (prion scrapie), appears to be the sole component of the infectious particle. They are responsible for transmissible spongiform encephalopathies, which affect both humans and animals. The mechanism of disease propagation is well understood and involves the interaction of PrPsc with its cellular isoform (PrPc) and subsequently abnormal structural conversion of the latter. PrPc is a glycoprotein anchored on the cell surface by a glycosylphosphatidylinositol moiety and expressed in most cell types but mainly in neurons. Prion diseases have been associated with the accumulation of the abnormally folded protein and its neurotoxic effects; however, it is not known if PrPc loss of function is an important component. New efforts are addressing this question and trying to characterize the physiological function of PrPc. At least four different mouse strains in which the PrP gene was ablated were generated and the results regarding their phenotype are controversial. Localization of PrPc on the cell membrane makes it a potential candidate for a ligand uptake, cell adhesion and recognition molecule or a membrane signaling molecule. Recent data have shown a potential role for PrPc in the metabolism of copper and moreover that this metal stimulates PrPc endocytosis. Our group has recently demonstrated that PrPc is a high affinity laminin ligand and that this interaction mediates neuronal cell adhesion and neurite extension and maintenance. Moreover, PrPc-caveolin-1 dependent coupling seems to trigger the tyrosine kinase Fyn activation. These data provide the first evidence for PrPc involvement in signal transduction.
Martins
V R
VR
Centro de Tratamento e Pesquisa, Hospital do Câncer, Universidade de São Paulo, São Paulo, SP, Brasil.
Mercadante
A F
AF
Cabral
A L
AL
Freitas
A R
AR
Castro
R M
RM
D000818:Animals; D003300:Copper / Q000378:metabolism; D004705:Endocytosis; D006801:Humans; D007797:Laminin / Q000502:physiology; D008024:Ligands; D008565:Membrane Proteins / Q000235:genetics / Q000502:physiology*; D051379:Mice; D010641:Phenotype; D018621:PrPC Proteins / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D018620:PrPSc Proteins / Q000235:genetics; D017096:Prion Diseases / Q000503:physiopathology*; D015398:Signal Transduction
D007797:Laminin; D008024:Ligands; D008565:Membrane Proteins; D018621:PrPC Proteins; D018620:PrPSc Proteins; D003300:Copper