Determination of 3-chloro-1,2-propanediol in foods and food ingredients by gas chromatography with mass spectrometric detection: collaborative study.
The results of a collaborative study are reported for the determination of 3-chloro-1,2-propanediol (3-monochloropropane-1,2-diol; 3-MCPD) in a wide range of foods and food ingredients, using gas chromatography with mass spectrometric detection and incorporating the use of a deuterated internal standard. After a pretrial study, 12 laboratories (6 United Kingdom, 1 Switzerland, 1 Japan, 2 United States, 1 The Netherlands, and 1 from the European Commission) were asked to analyze 12 test materials (as known duplicates or split-level samples) by using a prescribed procedure. The test materials consisted of duplicate samples of acid-hydrolyzed vegetable protein (containing 3-MCPD at 0.029 mg/kg), malt extract (0.055 mg/kg), wholemeal bread crumbs (0.030 mg/kg), salami (0.016 mg/kg), cheese alternative (0.043 mg/kg), and soup powder (split levels at 0.045 and 0.041 mg/kg). Repeatability ranged from 0.005 to 0.013 mg/kg and reproducibility, from 0.010 to 0.027 mg/kg, for the samples tested. Precision values were well within statistically predicted levels (HORRAT values of <1 for 5 of the 6 matrixes tested) and within method criteria prepared by a joint working group composed of the United Kingdom Ministry of Agriculture, Fisheries and Food and industry representatives. The study demonstrated the satisfactory validation of the method for quantifying 3-MCPD at levels of > or = 0.010 mg/kg. The limit of detection derived from separate in-house studies was estimated to be 0.005 mg/kg. The method was adopted First Action by AOAC INTERNATIONAL.
Brereton
P
P
Ministry of Agriculture, Fisheries and Food, Central Science Laboratory, Norwich, UK.
Kelly
J
J
Crews
C
C
Honour
S
S
Wood
R
R
Davies
A
A
D000821:Animal Feed / Q000032:analysis; D002138:Calibration; D002611:Cheese / Q000032:analysis; D002849:Chromatography, Gas; D002523:Edible Grain / Q000737:chemistry; D005433:Flour / Q000032:analysis; D005504:Food Analysis; D008401:Gas Chromatography-Mass Spectrometry; D007202:Indicators and Reagents; D008460:Meat / Q000032:analysis; D000517:alpha-Chlorohydrin / Q000032:analysis*
D007202:Indicators and Reagents; D000517:alpha-Chlorohydrin