Structure of the extracellular polysaccharide produced by Lactobacillus delbrueckii subsp. bulgaricus 291.
The lactic acid bacterium Lactobacillus delbrueckii subsp. bulgaricus 291, when grown in skimmed milk, produced 80 mg/L exopolysaccharide with an average molecular mass of 1.4 x 10(3) kDa. Monosaccharide analysis, methylation analysis, MS, and 1D/2D NMR (1H and 13C) studies performed on the native polysaccharide, and on oligosaccharides obtained from a mild acid hydrolysate of the native polysaccharide, showed the polysaccharide to consist of branched pentasaccharide repeating units with the following structure: [structure: see text].
Faber
E J
EJ
Department of Bio-Organic Chemistry, Bijvoet Center, Utrecht University, The Netherlands.
Kamerling
J P
JP
Vliegenthart
J F
JF
D000818:Animals; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002845:Chromatography; D007778:Lactobacillus / Q000737:chemistry / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008892:Milk / Q000382:microbiology; D008969:Molecular Sequence Data; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D011135:Polysaccharides, Bacterial