title: Inhibition kinetics of green crab (Scylla serrata) alkaline phosphatase by zinc ions: a new type of complexing inhibition.
abstract: The Tsou method was used to study the kinetic course of inactivation of green crab alkaline phosphatase by zinc ions. The results show that the enzyme was inactivated by a complexing scheme which has not been previously identified. The enzyme first reversibly and quickly binds Zn(2+) and then undergoes a slow reversible course to inactivation and slow conformational change. The inactivation reaction is a single molecule reaction and the apparent inactivation rate constant is for a saturated reaction being independent of Zn(2+) concentration if the concentration is sufficiently high. The microscopic rate constants of inactivation and the association constant were determined from the measurements.
authors.affiliation: Department of Biological Science and Biotechnology, Center for Ocean Science and Engineering, Tsinghua University, Beijing, China.
authors.forename: R Q
authors.initials: RQ
authors.lastname: Zhang
authors.forename: Q X
authors.initials: QX
authors.lastname: Chen
authors.forename: R
authors.initials: R
authors.lastname: Xiao
authors.forename: L P
authors.initials: LP
authors.lastname: Xie
authors.forename: X G
authors.initials: XG
authors.lastname: Zeng
authors.forename: H M
authors.initials: HM
authors.lastname: Zhou
mesh_terms: D000469:Alkaline Phosphatase / Q000037:antagonists & inhibitors* / Q000737:chemistry; D000814:Aniline Compounds / Q000494:pharmacology; D000818:Animals; D003386:Brachyura / Q000201:enzymology*; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D007700:Kinetics; D009943:Organophosphorus Compounds / Q000494:pharmacology; D011487:Protein Conformation / Q000187:drug effects; D019345:Zinc Acetate / Q000494:pharmacology*
chemical_list: D000814:Aniline Compounds; D004791:Enzyme Inhibitors; D009943:Organophosphorus Compounds; C068798:4-aminophenylphosphate; D000469:Alkaline Phosphatase; D019345:Zinc Acetate