An efficient method for the culture of Helicobacter pylori from gastric biopsies with two-section petri dishes.
OBJECTIVE
Helicobacter pylori is the principal cause of chronic gastritis and is strongly associated with peptic ulcer disease and gastric cancer, as well as with gastric lymphoma (mucosa-associated lymphoid tissue [MALT] type). H. pylori could be recovered by many kinds of media, however, it could not be detected efficiently. This study was trying to ameliorate the isolation and detection rate with selective and non-selective media.


METHODS
First, type strain (ATCC 43504) was cultured with twelve different kinds of sheep blood agar based on brain heart infusion medium for five days. Then five better non-selective media and five selective media were chosen for the biopsy, which were incubated at 37 degrees C in a microaerophilic incubator for five days. Endoscopic biopsies were collected from 623 patients at Kaohsiung Medical University Hospital.


RESULTS
It was found that non-selective media, which contain 5% modified chocolate agar (the ratio of fresh and heated sheep blood is 2) with 1% Isovitalex, had the best detection rate (91.1 +/- 0.6)%. Furthermore, 5% modified chocolate agar with 1% Isovitalex and 1% antibiotics would increase the detection rate to (97.1 +/- 0.3)%.


CONCLUSIONS
According to Ansorg's method, the best detection rate (98.4 +/- 0.7)% could be obtained with two-section petri dishes which had 5% modified chocolate agar with antibiotics and the other without antibiotics.
Ding
H J
HJ
School of Technology for Medical Sciences, Kaohsiung Medical University, Kaohsiung City, Taiwan.
Liu
Y C
YC
Peng
C F
CF
Wang
W M
WM
Chen
Y W
YW
Huang
Y F
YF
Lin
C C
CC
Chen
C Y
CY
D000818:Animals; D001431:Bacteriological Techniques / Q000379:methods; D015169:Colony Count, Microbial; D003470:Culture Media; D005753:Gastric Mucosa / Q000382:microbiology; D016480:Helicobacter pylori / Q000254:growth & development* / Q000302:isolation & purification; D006801:Humans; D012756:Sheep
D003470:Culture Media