title: Dietary cholesterol is secreted in intestinally derived chylomicrons during several subsequent postprandial phases in healthy humans.
abstract: BACKGROUND
  The process of intestinal absorption and chylomicron resecretion of dietary cholesterol in humans is poorly understood.
  
  
  OBJECTIVE
  The present study aimed to test the hypothesis that dietary cholesterol ingested during a given meal is resecreted into chylomicrons (and plasma) during several subsequent postprandial periods.
  
  
  METHODS
  Seven healthy subjects ingested 3 comparable mixed test meals (at 0, 8, and 24 h) containing a given amount of fat (49 g) and cholesterol (157 mg); blood samples were taken 3 and 6 h after each test meal and 48 and 72 h after the beginning of the experiment. Heptadeuterated dietary cholesterol was present in the first test meal only, enabling its specific determination with use of gas chromatography-mass spectrometry. Chylomicrons, LDL, and HDL were isolated and lipids were quantified.
  
  
  RESULTS
  In apolipoprotein B-48-containing chylomicrons, deuterated cholesterol concentrations were moderate after the first meal (1.3 x 10(-4) mmol/L), reached a maximum after the second meal (2.4 x 10(-4) mmol/L), and were still elevated after the third meal (1.7 x 10(-4) mmol/L). In plasma, LDL and HDL cholesterol enrichment in deuterated cholesterol was lower than in chylomicrons and plateaued after 24--48 h. Estimates of newly secreted exogenous deuterated cholesterol in chylomicrons indicate that 30.7%, 55.2%, and 14.1% of the total was secreted after the first, second, and third meals, respectively.
  
  
  CONCLUSIONS
  Ingested dietary cholesterol is secreted by the small intestine in chylomicrons into the circulation during > or =3 subsequent postprandial periods in healthy humans. This likely results from a complex multistep intestinal processing of cholesterol with dietary fat as a driving force.
authors.affiliation: Unité 476, Human Nutrition and Lipids, INSERM, Marseille, France.
authors.forename: G
authors.initials: G
authors.lastname: Beaumier-Gallon
authors.forename: C
authors.initials: C
authors.lastname: Dubois
authors.forename: M
authors.initials: M
authors.lastname: Senft
authors.forename: M F
authors.initials: MF
authors.lastname: Vergnes
authors.forename: A M
authors.initials: AM
authors.lastname: Pauli
authors.forename: H
authors.initials: H
authors.lastname: Portugal
authors.forename: D
authors.initials: D
authors.lastname: Lairon
mesh_terms: D000328:Adult; D053283:Apolipoprotein B-48; D001055:Apolipoproteins B / Q000097:blood; D002784:Cholesterol / Q000097:blood; D002791:Cholesterol, Dietary / Q000493:pharmacokinetics*; D002914:Chylomicrons / Q000097:blood / Q000378:metabolism*; D003903:Deuterium; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007408:Intestinal Absorption; D007421:Intestine, Small / Q000502:physiology; D008075:Lipoproteins, HDL / Q000097:blood; D008077:Lipoproteins, LDL / Q000097:blood; D008297:Male; D019518:Postprandial Period / Q000502:physiology*; D012016:Reference Values; D014280:Triglycerides / Q000097:blood
chemical_list: D053283:Apolipoprotein B-48; D001055:Apolipoproteins B; D002791:Cholesterol, Dietary; D002914:Chylomicrons; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D014280:Triglycerides; D002784:Cholesterol; D003903:Deuterium