title: Stability of fluoxetine in stored plasma, aqueous, and methanolic solutions determined by HPLC with UV detection.
abstract: The stability of fluoxetine was studied in plasma, and in aqueous and methanolic solutions at a concentration of 6 micrograms/ml under different storage temperatures and time intervals up to three months. Fluoxetine exhibited good stability at -20 and 5 degrees C, but was unstable at room temperature under the same conditions. A significant loss was observed at the second, third and fifth weeks in plasma, aqueous solution and methanolic solution respectively (P > 0.95). At the end of the experiment, the amount of fluoxetine-recovered was at least 55.25% regardless of the storage conditions. Chromatography was performed using a C8 column and the mobile phase consisted of methanol/acetonitrile/triethylamine solution (35:20:45) adjusted to pH 5.5. UV detection was at 230 nm. Fluoxetine was isolated from plasma by liquid-liquid extraction (LLE) with dichloromethane as extracting solvent after addition of 20% ammonia solution. The standard curve was linear over the range of 0.05-10 micrograms/ml. The inter-day coefficient of variation and the lower detectable limit were 6.92% and 0.05 microgram/ml respectively.
authors.affiliation: Forensic Medicine and Toxicology Division, Department of Pathology, Microbiology, and Forensic Medicine, Faculty of Medicine, University of Jordan, Amman, Jordan.
authors.forename: I A
authors.initials: IA
authors.lastname: Binsumait
authors.forename: K A
authors.initials: KA
authors.lastname: Hadidi
authors.forename: S A
authors.initials: SA
authors.lastname: Raghib
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004355:Drug Stability; D005473:Fluoxetine / Q000032:analysis* / Q000097:blood; D006801:Humans; D007202:Indicators and Reagents; D000432:Methanol; D017367:Selective Serotonin Reuptake Inhibitors / Q000032:analysis* / Q000097:blood; D012996:Solutions; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet; D014867:Water
chemical_list: D005473:Fluoxetine; D007202:Indicators and Reagents; D000432:Methanol; D017367:Selective Serotonin Reuptake Inhibitors; D012996:Solutions; D012997:Solvents; D014867:Water