[Co-integration of BLG-LAtPA and WAP improved the expression of LAtPA in transgenic mouse milk].
In order to improve the expression of longer acting tissue plasminogen activator in the mammary epithelium of transgenic mice, the fragment of BLG-LAtPA hydrid gene was microinjected into mouse embryos with mice whey acid protein gene. Three mouse were tested as being Co-integration of BLG-LAtPA and WAP transgene by PCR and Southern blot. Milk obtained from lactating females contains biologically active tPA, and the concentration of tPA was calculated to be about 10 micrograms/mL.
Zhou
J
J
Institute of Beijing Biotechnology, Beijing 100071, China.
Deng
J X
JX
Cheng
X
X
Lu
Y F
YF
Yang
X
X
Huang
P T
PT
D000818:Animals; D005260:Female; D007782:Lactoglobulins / Q000235:genetics*; D051379:Mice; D008822:Mice, Transgenic; D008845:Microinjections; D008892:Milk / Q000378:metabolism*; D008894:Milk Proteins / Q000235:genetics*; D010959:Tissue Plasminogen Activator / Q000096:biosynthesis* / Q000235:genetics
D007782:Lactoglobulins; D008894:Milk Proteins; C024471:whey acidic proteins; D010959:Tissue Plasminogen Activator