[Research on the selecting suspension cell line of higher productivity of flavonol glycoside by hypoxia stress as well as the stability in subcultures].
Investigate the influence of culture media to growth and flavonol glycoside synthesis of calli introduced from seedling of Ginkgo biloba. 6 cell lines were selected from calli by hypoxia stress. Among these cell lines the best one TZ-1 which growth index was 4.12 and the flavonol glycoside content was 1.25% in dried cell which was enhanced 257.1% compared with callus. The stability in subcultures was investigated: The average content of flavonol glycoside was 1.25% in dried cells and the growth index was 3.99 during 6 subcultures. Which variation coefficient was separately 0.065 and 0.048. The results show that hypoxia stress is a efficient method to select suspension cell line of higher productivity of flavonol glycoside.
Liu
J J
JJ
College of Chemistry and Chemical Engineering, Central-south University of Technology, Changsha 410082, China.
Guo
Y
Y
Zheng
S P
SP
Zhang
M H
MH
D015687:Cell Hypoxia; D002460:Cell Line; D003470:Culture Media; D005419:Flavonoids / Q000096:biosynthesis*; D044948:Flavonols; D020441:Ginkgo biloba / Q000378:metabolism*; D006027:Glycosides / Q000096:biosynthesis*; D010946:Plants, Medicinal; D013535:Suspensions
D003470:Culture Media; D005419:Flavonoids; D044948:Flavonols; D006027:Glycosides; D013535:Suspensions