[Determination of tranilast in human plasma by reversed-phase high performance liquid chromatography and its pharmacokinetics].
A simple and rapid method for the determination of tranilast in human plasma by RP-HPLC is described. The analytical column was packed with YWG-C18. The mobile phase consisted of methanol-0.02 mol/L KH2PO4(60:40, V/V; pH 4.2), and detected by UV detector at a wavelength of 333 nm. The plasma sample was injected directly into the HPLC system after precipitation of the protein with methanol. The calibration curve was linear within the concentration range of 0.625-40 mg/L. The average recovery was 100.0% +/- 4.1%, and minimum detectable concentration was 0.2 mg/L. By using this method, the pharmacokinetics of tranilast in the plasma of ten healthy volunteers after oral administration of 200 mg drug were studied. The plasma drug concentration-time curve of volunteers conforms to one-compartment open model.
Zhao
F
F
General Hospital of Nanjing Command, PLA, Nanjing, 210002.
Yuan
Y
Y
Tan
L
L
Zhang
X
X
D018926:Anti-Allergic Agents / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D062367:ortho-Aminobenzoates / Q000097:blood* / Q000493:pharmacokinetics
D018926:Anti-Allergic Agents; D062367:ortho-Aminobenzoates; C012293:tranilast