Immunohistochemical investigation with carbohydrate monoclonal antibodies in cystic fibrosis.
BACKGROUND
Cystic fibrosis is one of the most common inherited diseases in Europe. In patients with cystic fibrosis, the serum levels of the tumour marker antigens CA19-9 and CA50, which are normally found in the epithelium of many organs, are increased. No such finding has been reported for CA242, another ubiquitous tumour marker antigen. We set out to investigate immunohistochemically the correlation of CA19-9 and CA50 with cystic fibrosis and to compare the effect of antibodies against these three tumour markers with regard to improved immunohistochemical results.


METHODS
Paraffin-embedded tissue from different organs of 18 patients with cystic fibrosis was studied. Immunohistochemical reactions were performed with antibodies against CA19-9, CA50 and CA242 followed by visualisation with the ABC method.


RESULTS
Immunohistochemical reactions in different tissues were uniform. Anti-CA19-9 reacted less intensive than anti-CA50 and anti-CA242. The antibodies generally bound to cell membranes and compounds of the cytoplasm.


CONCLUSIONS
This study demonstrates immunohistochemically the epitopes of monoclonal carbohydrate antigens (CA-19-9, CA-50 and CA 242) in cystic fibrosis for the first time. We also supposed that cystic fibrosis patients show increased serum levels of CA242. However, none of the three antibodies investigated seems suitable for the immunohistochemical diagnosis of cystic fibrosis.
Makovitzky
J
J
Universität Rostock, Medizinische Fakultät, Universitäts Frauenklinik, Doberaner Str., 142, 18057 Rostock, Germany.
Götze
S
S
D000911:Antibodies, Monoclonal / Q000276:immunology; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000032:analysis*; D018395:CA-19-9 Antigen / Q000032:analysis*; D002241:Carbohydrates / Q000276:immunology; D003550:Cystic Fibrosis / Q000378:metabolism* / Q000473:pathology; D018450:Disease Progression; D006801:Humans; D007150:Immunohistochemistry
D000911:Antibodies, Monoclonal; D015295:Antigens, Tumor-Associated, Carbohydrate; C074601:CA 242 antigen; D018395:CA-19-9 Antigen; C082699:CA-50 antigen; D002241:Carbohydrates