Polyaniline-dacron composite as solid phase in enzyme linked immunosorbent assay for Yersinia pestis antibody detection.
Polyaniline (PANI) was chemically synthesized on a dacron disk surface and an antigen (F1 fraction) obtained from Yersinia pestis was covalently fixed onto this composite via glutaraldehyde. The enzyme linked immunosorbent assay (ELISA) or rapid ELISA procedure detected immunoglobulin G (IgG) anti-F1 fraction in human serum employing this derivative. The appropriate conditions for carrying out the test were established as an antigen concentration of 2 microg/PANI-dacron disk, peroxidase labeled goat anti-human IgG conjugate diluted 4000 times, and a serum dilution of 1:100. The PANI-dacron disks showed greater antigen retention than conventional poly(vinyl chloride) plates and less antibody unspecific adsorption.
Coêlho
R A
RA
Departamento de Bioquímica and Laboratório de Imunopatologia Keizo Asami, Universidade Federal de Pernambuco, Cidade Universitária, 50670-420 Recife, PE, Brazil.
Santos
G M
GM
Azevêdo
P H
PH
Jaques
G de A
Gde A
Azevedo
W M
WM
Carvalho
L B
LB
D000814:Aniline Compounds / Q000138:chemical synthesis / Q000737:chemistry; D000907:Antibodies, Bacterial / Q000032:analysis*; D001426:Bacterial Proteins / Q000097:blood / Q000276:immunology*; D020099:Coated Materials, Biocompatible; D004355:Drug Stability; D004797:Enzyme-Linked Immunosorbent Assay; D004853:Epoxy Resins; D006801:Humans; D010930:Plague / Q000276:immunology*; D011093:Polyethylene Terephthalates / Q000737:chemistry; D011095:Polyethylenes; D013997:Time Factors; D015010:Yersinia pestis / Q000276:immunology*
D000814:Aniline Compounds; D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D020099:Coated Materials, Biocompatible; D004853:Epoxy Resins; D011093:Polyethylene Terephthalates; D011095:Polyethylenes; C086725:caf1 protein, Yersinia pestis; C416807:polyaniline; C049486:solid water polymer