24-hour 51Cr post-transfusion survival, 51Cr life span and haemolysis of red blood cells stored at 4 degrees C for 56 days in AS-3.
OBJECTIVE
Red blood cells (RBC) were collected either by a manual method using a 16-gauge needle or by an apheresis procedure using an 18-gauge needle, and were stored at 4 degrees C in a solution of CP2D (anticoagulant)/AS-3 (Nutricel) for 56 days. The purpose was to compare the outcome of the autotransfused red cells collected by both techniques.


METHODS
Five healthy male volunteers were studied on two occasions.


RESULTS
The autotransfusions of the manual and apheresed RBC resulted in a mean 24-h post-transfusion survival of 71%, a normal mean 51Cr RBC life span, a 2,3 DPG level that was less than 10% of normal, and 0.6% haemolysis.


CONCLUSIONS
Whether collected manually or by apheresis, the outcomes were similar for RBC stored at 4 degrees C for 56 days in CP2D/AS-3.
Valeri
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Naval Blood Research Laboratory, Boston University School of Medicine, 615 Albany Street, Boston, MA 02118, USA. navblood@BU.edu
Pivacek
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Cassidy
G P
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Ragno
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D019794:2,3-Diphosphoglycerate / Q000097:blood; D000225:Adenine / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000097:blood; D000328:Adult; D000925:Anticoagulants / Q000494:pharmacology; D001781:Blood Component Removal; D001793:Blood Preservation; D002469:Cell Separation; D002470:Cell Survival; D002498:Centrifugation; D002860:Chromium Radioisotopes; D002951:Citrates / Q000494:pharmacology; D015925:Cryopreservation; D004905:Erythrocyte Aging; D004912:Erythrocytes / Q000737:chemistry*; D005947:Glucose / Q000494:pharmacology; D006454:Hemoglobins / Q000032:analysis; D006461:Hemolysis; D006801:Humans; D008297:Male; D008353:Mannitol / Q000494:pharmacology; D018962:Phlebotomy; D011188:Potassium / Q000097:blood; D012965:Sodium Chloride / Q000494:pharmacology; D013997:Time Factors
C037839:ADSOL; D000925:Anticoagulants; D002860:Chromium Radioisotopes; D002951:Citrates; D006454:Hemoglobins; D019794:2,3-Diphosphoglycerate; D008353:Mannitol; D012965:Sodium Chloride; C007077:citrate phosphate dextrose; D000255:Adenosine Triphosphate; D005947:Glucose; D000225:Adenine; D011188:Potassium