title: Prolactin inhibition of FSH-induced tissue type plasminogen activator expression in cultured rat granulosa cells.
abstract: This study was designed to investigate whether prolactin (PRL) affects coordinated regulation of tissue plasminogen activator (tPA) and plasminogen activator inhibitor type-I(PAI-I) gene expression in rat granulosa cells in vitro. Several methods, such as SDS-PAGE, immunoblot etc. were used to detect the effect of PRL on tPA and PAI-I gene expression. The results demonstrated: (1) PRL increases PAI-I mRNA production in cultured granulosa cells. Inclusion of follicle stimulating hormone (FSH) with PRL has a synergistic effect on the increase of PAI-I mRNA levels. After 48 h culture in the presence of FSH with PRL, a 7.8-fold increase in PAI-I mRNA levels is observed as compared with PRL alone. The synergistic increase in PAI-I mRNA levels occurs in a dose- and time-dependent manner; (2) the increase in PAI-I mRNA synthesis in the cells by PRL alone, or PRL in combination with FSH, is well correlated with the changes of PAI-I activity levels in the conditioned media; (3) PRL in culture also decreases FSH-induced tPA activity level in a dose-dependent fashion. The decrease in FSH-induced tPA activity level by PRL is correlated with an increase in the amount of PA-PAI-I complexes in the conditioned media. This suggests that the decline of tPA activity is related to neutralization of tPA by the increased PAI-I activity in the media.
authors.affiliation: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080. Liuyx@panda.ioz.ac.cn
authors.forename: Y X
authors.initials: YX
authors.lastname: Liu
authors.forename: H Z
authors.initials: HZ
authors.lastname: Liu
authors.forename: Y J
authors.initials: YJ
authors.lastname: Chen
authors.forename: N Y
authors.initials: NY
authors.lastname: Tor
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D005260:Female; D005640:Follicle Stimulating Hormone / Q000037:antagonists & inhibitors*; D015870:Gene Expression; D006107:Granulosa Cells / Q000378:metabolism*; D006080:Ovarian Follicle / Q000378:metabolism; D017395:Plasminogen Activator Inhibitor 1 / Q000096:biosynthesis* / Q000235:genetics; D011388:Prolactin / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D010959:Tissue Plasminogen Activator / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D017395:Plasminogen Activator Inhibitor 1; D012333:RNA, Messenger; D011388:Prolactin; D005640:Follicle Stimulating Hormone; D010959:Tissue Plasminogen Activator