title: A novel retroviral vector that allows the magnetic selection of infected cells.
abstract: Retroviral vectors are used widely in research and are also being designed for use in gene therapy trials. In practice, these vectors usually contain a marker gene, which is often a drug selection gene. In this report, a novel retroviral vector has been constructed which contains a gene that allows selection for infected cells by a magnet. This gene is a single-chain antibody (sFv) to a specific hapten molecule 4-ethoxymethylene-2-phenyl-2-oxazolin-5-one (phOx). sFv specific for phOx is displayed on the surface of infected cells. This feature allows binding to phOx-BSA coated magnetic beads which are used to isolate the infected cells.
authors.affiliation: Department of Microbiology and Immunology, Markey Cancer Center, University of Kentucky, Lexington, KY40536-0096, USA. jzhanl@pop.uky.edu
authors.forename: J
authors.initials: J
authors.lastname: Zhang
authors.forename: C M
authors.initials: CM
authors.lastname: Sapp
mesh_terms: D002460:Cell Line; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins; D006241:Haptens / Q000276:immunology; D006801:Humans; D007128:Immunoglobulin Fragments / Q000235:genetics; D007135:Immunoglobulin Variable Region / Q000235:genetics; D018189:Immunomagnetic Separation / Q000379:methods*; D008164:Luminescent Proteins / Q000235:genetics; D008280:Magnetics; D010080:Oxazoles / Q000276:immunology; D012190:Retroviridae
chemical_list: C119678:4-ethoxymethylene-2-phenyl-2-oxazoline-5-one; D006241:Haptens; D007128:Immunoglobulin Fragments; D007135:Immunoglobulin Variable Region; D008164:Luminescent Proteins; D010080:Oxazoles; C055966:immunoglobulin Fv; D049452:Green Fluorescent Proteins