Induction of specific cytotoxic T-cell activity against xenogeneic target cells in carp (Cyprinus carpio).
OBJECTIVE
To investigate the induction of cytotoxic T cells in carp (Cyprinus carpio) after inoculation of fish with 2 xenogeneic line cells and to examine specificity of the cytotoxic activity.


METHODS
22 carp.


METHODS
Fish were inoculated with mouse myeloma line cells P3.NS-1/1Ag4.1 (NS-1) or chicken Marek's disease tumor-derived lymphoma line cells (MDCC MSB-1). Cytotoxic activity of immune lymphocytes was evaluated by incubating effector cells with homologous and heterologous target cells. Populations of effector cells were identified by blocking T-lymphocytes from effector cells, using anti-carp T-cell monoclonal antibody and complement.


RESULTS
Lymphocytes in blood, spleen, and head kidney of carp inoculated with NS-1 cells or MDCC MSB-1 cells had dose-dependent cytotoxic effects against homologous target cells but not against heterologous target cells. Lymphocytes from noninoculated carp did not have cytotoxic effects. Depletion of T-lymphocytes in spleen cells from NS-1-inoculated carp resulted in a decrease of cytotoxic activity against NS-1 cells. Cytotoxic activity of spleen lymphocytes from NS-1-inoculated or noninoculated carp was not evident when cytotoxic tests were performed after addition of anti-NS-1 carp serum.


CONCLUSIONS
Inoculation with xenogeneic target cells induces a specific cytotoxic T-cell response in carp. Thus, cell-mediated immunity plays a role in defense against infection of parasitic organisms such as protozoa and helminths.
Yamamoto
K
K
Division of Veterinary Science, Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University, Osaka, Japan.
Mukamoto
M
M
Watarai
S
S
Kodama
H
H
Nakayasu
C
C
Okamoto
N
N
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D015478:Antigens, Heterophile / Q000276:immunology*; D002347:Carps / Q000276:immunology*; D002645:Chickens; D003165:Complement System Proteins / Q000276:immunology; D003602:Cytotoxicity, Immunologic / Q000276:immunology; D018984:Epitopes, T-Lymphocyte / Q000276:immunology; D005434:Flow Cytometry / Q000662:veterinary; D007770:L-Lactate Dehydrogenase / Q000032:analysis / Q000096:biosynthesis; D008380:Marek Disease / Q000276:immunology; D051379:Mice; D009101:Multiple Myeloma / Q000276:immunology; D013154:Spleen / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D015478:Antigens, Heterophile; D018984:Epitopes, T-Lymphocyte; D003165:Complement System Proteins; D007770:L-Lactate Dehydrogenase