[Determination of vitamin D3 in health-care foods fortified with calcium by reversed-phase high performance liquid chromatography].
"Bu Gai Bao" and "Gaoxiao Man Gai" are health-care foods fortified with calcium for people of all ages. This paper describes a method of reversed-phase high performance liquid chromatography for quantitating vitamin D3 in these products. The method is simple, sensitive, and suitable for routine analysis of vitamin D3 in a variety of health-care foods fortified with calcium. Shimadzu LC-4A high performance liquid chromatograph with SPD-2AS UV detector and C-R2AX data processor were used. The vitamin D3 in products was extracted with chloroform and separated with mu-Bondapak C18 column(300 mm x 3.9 mm i.d.) and methanol water(87:13, V/V) mobile phase with detection at UV-264 nm. Regression coefficient of correlation of calibration curves in the range of 10-50 mg/L was 0.9998. Average recoveries of vitamin D3 added to the products was 95.63%. The coefficients of variation of vitamin D3 in both products were 0.24%-0.49%.
Wu
H
H
Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, 350002.
Tang
G
G
D002118:Calcium; D002762:Cholecalciferol / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005527:Food, Fortified / Q000032:analysis*
D002762:Cholecalciferol; D002118:Calcium