[Study of the inhibitory effects of retrovirus-mediated p16 gene on human ovarian cancer cell line].
OBJECTIVE
To study the inhibitory effects of retro-virus-mediated p16 gene on human ovarian cancer cell line CAOV3.


METHODS
Recombinant eukaryotic expression vector pDOR-p16 containing exogenous human wt-p16 cDNA and vector with neomycin resistance gene only were introduced by lipofectamine-mediated gene transfection into CAOV3 cell line which does not express p16 endogenously. By using revert-polymerase chain reaction amplification, mRNA in situ hybridization and immunocytochemistry, the clones obtained were detected for their efficiency of transfection and effects of vector expression and observed for their biologic behavior.


RESULTS
Exogenous wt-p16 had successfully been transferred into CAVO3 cells and obtained permanent expression. The growth rate of these transfected CAOV3 cells in regular medium and soft agar was inhibited, and the tumorigenicity in nude mice showed that two in four mice failed to form tumor and the others suffered from tumor later than contrast group by 7 to 14 days. The percentage of phase G1 cells increased and that of phase S cells decreased by analysing cell cycle. The ultrastructural changes of the cells were observed under electron microscope, revealing necrosis and growth retardation.


CONCLUSIONS
p16 gene played an important role in generation and development of ovarian carcinoma. This study might provide the experimental evidence for the gene therapy in human ovarian cancer.
Wang
M
M
Second Clinical College, China Medical University, Shenyang 110003.
Jiang
S
S
Kong
B
B
D002455:Cell Division; D018284:Cystadenocarcinoma, Serous / Q000235:genetics / Q000473:pathology*; D005260:Female; D019942:Genes, p16 / Q000235:genetics*; D015316:Genetic Therapy; D006801:Humans; D010051:Ovarian Neoplasms / Q000235:genetics / Q000473:pathology*; D014162:Transfection; D014407:Tumor Cells, Cultured