Lack of effect of the flavonoids, myricetin, quercetin, and rutin, on repair of H2O2-induced DNA single-strand breaks in Caco-2, Hep G2, and V79 cells.
In the present study the effects of three flavonoids on the repair of H2O2-induced DNA strand breaks were investigated in Caco-2, Hep G2, and V79 cells. At the concentrations used, myricetin, quercetin, rutin, and H2O2 did not significantly affect cell viability in all the cell lines. Catalase activity was measured in V79 cells and was found to be considerably lower than activities previously measured in Caco-2 and Hep G2 cells. Cells were exposed to 50 microM H2O2 for 0.5 hour at 37 degrees C. After treatment, DNA strand break repair in H2O2-treated cells was monitored at various time points over a 48-hour period using the alkaline single-cell gel electrophoresis assay. Caco-2 cells repaired faster than Hep G2 cells, which repaired considerably faster than V79 cells. Preincubation with 50 microM quercetin for 24 hours significantly decreased the extent of H2O2-induced DNA single-strand breaks throughout repair time points in Caco-2 cells (p < 0.05), but not in Hep G2 cells. Myricetin (50 microM) and rutin (50 microM) had no effect on repair in Caco-2 and Hep G2 cells. Preincubation for 10 hours with quercetin and rutin, but not myricetin, significantly decreased the initial extent of DNA damage induced by H2O2 in V79 cells (p < 0.05). However, from the results of this study, none of the three flavonoids increased the rate of repair of strand breaks in any of the cell types.
Aherne
S A
SA
Nutritional Sciences, Department of Food Science and Technology, University College Cork, Republic of Ireland.
O'Brien
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NM
D018938:Caco-2 Cells; D002374:Catalase / Q000378:metabolism; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004249:DNA Damage / Q000187:drug effects*; D004260:DNA Repair / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004587:Electrophoresis, Agar Gel; D005419:Flavonoids / Q000494:pharmacology*; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity; D007700:Kinetics; D011794:Quercetin / Q000494:pharmacology*; D012431:Rutin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D005419:Flavonoids; D012431:Rutin; C040015:myricetin; D011794:Quercetin; D006861:Hydrogen Peroxide; D002374:Catalase