Electrical properties of a light-addressable microelectrode chip with high electrode density for extracellular stimulation and recording of excitable cells.
A light-addressable microelectrode chip with 3600 TiN electrodes was fabricated. Amorphous silicon (a-Si:H) serves as a photo conductor. The electrodes on the chip are addressed by a laser spot and electrical properties of the system are determined. DC measurements show a dark to bright dynamic of 10(6)-10(7). The AC impedance dynamic @ 1 kHz/100 mV and thus the signal-to-noise-ratio is determined to 60. This value is quite sufficient for electrophysiological measurements. For the first time, recordings from cardiac myocytes are reported using the principle of light-addressing. Measurements were done with a standard laser scan microscope (Zeiss LSM 410).
Bucher
V
V
Natural and Medical Sciences Institute, Markwiesenstrasse 55, D-72770, Reutlingen, Germany. bucher@nmi.de
Brunner
B
B
Leibrock
C
C
Schubert
M
M
Nisch
W
W
D004558:Electric Stimulation; D004563:Electrochemistry; D004573:Electrolytes; D008027:Light; D008839:Microelectrodes; D009206:Myocardium / Q000378:metabolism
D004573:Electrolytes