Proteomic dissection of dome formation in a mammary cell line: role of tropomyosin-5b and maspin.
In this work we extended the study of genes controlling the formation of specific differentiation structures called "domes" formed by the rat mammary adenocarcinoma cell line LA7 under the influence of DMSO. We have reported previously that an interferon-inducible gene, rat-8, and the beta-subunit of the epithelial sodium channel (ENaC) play a fundamental role in this process. Now, we used a proteomic approach to identify proteins differentially expressed either in DMSO-induced LA7 or in 106A10 cells. Two differentially expressed proteins were investigated. The first, tropomyosin-5b, strongly expressed in DMSO-induced LA7 cells, is needed for dome formation because its synthesis inhibition by the antisense RNA technology abolished domes. The second protein, maspin, strongly expressed in the uninduced 106A10 cell line, inhibits dome formation because 106A10 cells, transfected with rat8 cDNA (the function of which is required for the organization of these structures), acquired the ability to develop domes when cultured in presence of an antimaspin antibody. Dome formation in these cultures are accompanied by ENaC beta-subunit expression in the absence of DMSO. Therefore, dome formation requires the expression of tropomyosin-5b, in addition to the ENaC beta-subunit and the rat8 proteins, and is under the negative control of maspin.
Zucchi
I
I
Istituto Tecnologie Biomediche Avanzate, Consiglio Nazionale delle Ricerche, Via F. lli Cervi 93, 20090 Segrate-Milan, Italy. zucchi@itba.mi.cnr.it
Bini
L
L
Valaperta
R
R
Ginestra
A
A
Albani
D
D
Susani
L
L
Sanchez
J C
JC
Liberatori
S
S
Magi
B
B
Raggiaschi
R
R
Hochstrasser
D F
DF
Pallini
V
V
Vezzoni
P
P
Dulbecco
R
R
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D017931:DNA Primers; D053503:Epithelial Sodium Channels; D016147:Genes, Tumor Suppressor; D008321:Mammary Glands, Animal / Q000166:cytology / Q000378:metabolism*; D011506:Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000502:physiology*; D020543:Proteome; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D015843:Serpins / Q000235:genetics / Q000502:physiology*; D015222:Sodium Channels / Q000378:metabolism; D014335:Tropomyosin / Q000235:genetics / Q000502:physiology*; D014407:Tumor Cells, Cultured
D017931:DNA Primers; D053503:Epithelial Sodium Channels; D011506:Proteins; D020543:Proteome; D012333:RNA, Messenger; C085344:SERPIN-B5; C507050:Scnn1b protein, rat; D015843:Serpins; D015222:Sodium Channels; C498784:Tpm3 protein, rat; D014335:Tropomyosin