1,8-cineole protects against liver failure in an in-vivo murine model of endotoxemic shock.
The effects of 1,8-cineole on D-galactosamine/lipopolysaccharide (GalN/LPS)-induced shock model of liver injury was investigated in mice. The co-administration of GalN (700 mg kg(-1), i.p.) and LPS (5 microg kg(-1), i.p.) greatly elevated serum concentrations of tumour necrosis factor-alpha (TNF-alpha), alanine aminotransferase and aspartate aminotransferase, and induced massive hepatic necrosis and lethality in 100% of control mice. Pretreatment with 1,8-cineole (400 mg kg(-1), p.o.) and dexamethasone (1 mg kg(-1), s.c.), 60 min before GalN/LPS, offered complete protection (100%) against the lethal shock and acute elevation in serum TNF-alpha and serum transaminases. Hepatic necrosis induced by GalN/LPS was also greatly reduced by both 1,8-cineole and dexamethasone treatment. The results indicate that 1,8-cineole protects mice against GalN/LPS-induced liver injury through the inhibition of TNF-alpha production, and suggest that 1,8-cineole may be a promising agent to combat septic-shock-associated pathologies.
Santos
F A
FA
Department of Physiology and Pharmacology, Federal University of Ceará, Fortaleza, Brazil.
Silva
R M
RM
Tomé
A R
AR
Rao
V S
VS
Pompeu
M M
MM
Teixeira
M J
MJ
De Freitas
L A
LA
De Souza
V L
VL
D000818:Animals; D003511:Cyclohexanols; D004195:Disease Models, Animal; D000077591:Eucalyptol; D005688:Galactosamine / Q000008:administration & dosage / Q000009:adverse effects; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000009:adverse effects; D017093:Liver Failure / Q000209:etiology / Q000517:prevention & control*; D008297:Male; D008610:Menthol / Q000031:analogs & derivatives* / Q000494:pharmacology*; D051379:Mice; D039821:Monoterpenes; D012772:Shock, Septic / Q000150:complications / Q000503:physiopathology*; D012997:Solvents / Q000494:pharmacology*; D013729:Terpenes; D000637:Transaminases / Q000096:biosynthesis / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D003511:Cyclohexanols; D008070:Lipopolysaccharides; D039821:Monoterpenes; D012997:Solvents; D013729:Terpenes; D014409:Tumor Necrosis Factor-alpha; D008610:Menthol; D005688:Galactosamine; D000637:Transaminases; D000077591:Eucalyptol