Sphingomonas cloacae sp. nov., a nonylphenol-degrading bacterium isolated from wastewater of a sewage-treatment plant in Tokyo.
A nonylphenol-assimilating bacterium isolated at a sewage-treatment plant in Tokyo was studied phenotypically, genotypically and phylogenetically. Analysis of the 16S rDNA sequence, the G+C content of the DNA (63 mol%) and the isoprenoid quinone composition, as well as the presence of sphingoglycolipid and the whole-cell fatty acid profile, revealed that the isolate is a member of the genus Sphingomonas. However, the sequence similarity of the 16S rDNA with that of known Sphingomonas spp. was found to be at most 96%, implying that the isolate is distinctive. Furthermore, the results of DNA-DNA hybridization experiments and its physiological characteristics clearly indicated that the isolate represents a new Sphingomonas species, for which the name Sphingomonas cloacae is proposed; strain S-3T (= JCM 10874T = IAM 14885) is the type strain.
Fujii
K
K
Urano
N
N
Ushio
H
H
Satomi
M
M
Kimura
S
S
D015373:Bacterial Typing Techniques; D001673:Biodegradation, Environmental; D004275:DNA, Ribosomal / Q000235:genetics; D004783:Environmental Microbiology; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D010636:Phenols / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012722:Sewage / Q000382:microbiology*; D020578:Sphingomonas / Q000145:classification* / Q000378:metabolism*; D009626:Terminology as Topic
D004275:DNA, Ribosomal; D005227:Fatty Acids; D010636:Phenols; D012336:RNA, Ribosomal, 16S; D012722:Sewage; C025256:nonylphenol