Anthelmintic activity of the stem bark extracts of Berlina grandiflora and one of its active principles, Betulinic acid.
This study examines the anthelmintic activity of a methanol, hexane and ethylacetate extracts of Berlina grandiflora and purified betulinic acid, the major triterpenoid found in the extract. Caenorhabditis elegans, a free living soil nematode was used as in vitro model in the study. A suspension of worms was treated with the extracts. After seven days of incubation activity was assessed in terms of number of worms exhibiting motiliy. The results showed that methanol, hexane and ethylacetate fractions at 500 ppm showed anthelmintic activities in the order ethylacetate > methanol > hexane from the alcoholic crude extract. The isolated betulinic acid from the ethylacetate fraction at 100 and 500 ppm showed strong anthelmintic activities comparable to piperazine. These results confirm the traditional use of Berlina grandiflora as an anthelmintic and indicate that betulinic acid is the active component.
Enwerem
N M
NM
National Institute for Pharmaceutical Research and Development, Abuja, Nigeria. Nkyenwerem@aol.com
Okogun
J I
JI
Wambebe
C O
CO
Okorie
D A
DA
Akah
P A
PA
D000818:Animals; D000871:Anthelmintics / Q000494:pharmacology*; D000962:Antimalarials / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D017173:Caenorhabditis elegans / Q000187:drug effects*; D007887:Fabaceae / Q000737:chemistry*; D053978:Pentacyclic Triterpenes; D010879:Piperazines / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D018547:Plant Stems; D010946:Plants, Medicinal; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000094062:Betulinic Acid
D000871:Anthelmintics; D000962:Antimalarials; D053978:Pentacyclic Triterpenes; D010879:Piperazines; D010936:Plant Extracts; D014315:Triterpenes; C006589:piperazine citrate; D000094062:Betulinic Acid