Efficacy of nonwoven fabric bioreactor immobilized with porcine hepatocytes for ex vivo xenogeneic perfusion treatment of liver failure in dogs.
We have developed a new bioartificial liver bioreactor filled with porcine hepatocytes immobilized on polyester nonwoven fabric (NWF). In this study, we investigated the efficacy of our hybrid bioartificial liver system incorporating the NWF bioreactors and an immunoglobulin adsorbent column for perfusion treatment in a canine liver failure model. Xenogeneic perfusion treatment for operative canine liver failure models were performed for 3 h, and survival time, intracranial pressure, and blood and cerebrospinal fluid data were documented. Treatment was carried out without obstruction by immunological rejection when immunoglobulin adsorbent columns were used with the NWF bioreactors in combination. Dogs treated with this system exhibited a restricted increase of intracranial pressure and significant compensatory effects on blood and cerebrospinal amino acid imbalances as shown by a significant improvement of Fischer's ratio. On the other hand, relatively low capacity for ammonia elimination was shown as compared with homologous direct hemoperfusion.
Naruse
K
K
Division of Artificial Organs and Transplantation, Department of Surgery, Faculty of Medicine, University of Tokyo, Tokyo, Japan. narusek-sur@h.u-tokyo.ac.jp
Sakai
Y
Y
Lei
G
G
Sakamoto
Y
Y
Kobayashi
T
T
Puliatti
C
C
Aronica
G
G
Morale
W
W
Leone
F
F
Qiang
S
S
Ming
S G
SG
Ming
S
S
Li
Z
Z
Chang
S J
SJ
Suzuki
M
M
Makuuchi
M
M
D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D019149:Bioreactors; D001774:Blood Chemical Analysis; D018914:Cells, Immobilized; D002555:Cerebrospinal Fluid / Q000737:chemistry; D004285:Dogs; D006464:Hemoperfusion / Q000295:instrumentation; D022781:Hepatocytes; D007164:Immunosorbents; D007427:Intracranial Pressure; D017093:Liver Failure / Q000378:metabolism / Q000503:physiopathology / Q000628:therapy*; D019164:Liver, Artificial; D011091:Polyesters; D013552:Swine
D007164:Immunosorbents; D011091:Polyesters; D001219:Aspartate Aminotransferases