Cyanide-resistant alternative respiration is strictly correlated to intracellular peroxide levels in Acremonium chrysogenum.
A strict correlation between the intensity of the cyanide-resistant alternative respiratory pathway and the intracellular peroxide levels in the cephalosporin C producer filamentous fungus Acremonium chrysogenum was demonstrated. Intracellular peroxide levels increased in a dose-dependent manner after addition of H2O2 to the culture media. A similar phenomenon was observed due to the specific inhibition of catalase by salicylic acid. In both cases, cyanide-resistant respiration was markedly stimulated. On the other hand, both cyanide-resistant respiration and intracellular peroxide levels were effectively suppressed by the lipid peroxyl radical scavenger DL-alpha-tocopherol, which breaks lipid peroxidation chains effectively. Our findings firmly supported the assumption that there is a connection between the intracellular peroxide levels and the intensity of the alternative respiratory pathway in fungi.
Karaffa
L
L
Department of Microbiology and Biotechnology Faculty of Sciences University of Debrecen P.O. Box 63, H-4010, Debrecen, Hungary. karaffal@tigris.klte.hu
Váczy
K
K
Sándor
E
E
Biró
S
S
Szentirmai
A
A
Pócsi
I
I
D000164:Acremonium / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D002374:Catalase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D002511:Cephalosporins / Q000378:metabolism*; D003486:Cyanides / Q000494:pharmacology; D003580:Cytochromes / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D005452:Fluoresceins; D016166:Free Radical Scavengers; D005947:Glucose / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D007700:Kinetics; D003904:Mitosporic Fungi; D010100:Oxygen; D010101:Oxygen Consumption / Q000187:drug effects; D010545:Peroxides / Q000032:analysis / Q000378:metabolism*; D011048:Polarography; D020156:Salicylic Acid / Q000494:pharmacology*; D013050:Spectrometry, Fluorescence; D013997:Time Factors; D014810:Vitamin E / Q000494:pharmacology*
D002511:Cephalosporins; D003486:Cyanides; D003580:Cytochromes; D005452:Fluoresceins; D016166:Free Radical Scavengers; D010545:Peroxides; D014810:Vitamin E; C049375:perhydroxyl radical; C037631:2',7'-dichlorofluorescein; D006861:Hydrogen Peroxide; D002374:Catalase; D005947:Glucose; D020156:Salicylic Acid; D010100:Oxygen