Kinetic differences of purified laccases from six Pleurotus ostreatus strains.
OBJECTIVE
Enzyme kinetics of purified laccases from six different Pleurotus ostreatus strains were determined in the oxidation of syringaldazine, guaiacol and ABTS.


RESULTS
Significant differences in the kinetic constants were found. Catalytic activity (kcat) ranged from 19 to 941 U mg(-1) for syringaldazine, from 18 to 1565 U mg(-1) for ABTS, and from 4 to 44 U mg(-1) for guaiacol. The apparent affinity constants (KM) also showed significant differences between the different strains, from 12 to 52 micromol l(-1) for syringaldazine, from 8 to 79 micromol l(-1) for ABTS, and from 0.46 to 6.61 mmol l(-1) for guaiacol. No differences were found either on the effect of increasing concentrations of organic solvent (acetonitrile) or on the activity pH profile. The temperature profile was the same for all the P. ostreatus strains, except for the IE8 strain, which seems to be more sensitive to temperature. The kinetic and stability data from the six P. ostreatus strains were also compared with those obtained from other white rot fungi, Coriolopsis gallica and Trametes versicolor, showing clear differences.


CONCLUSIONS
The different P. ostreatus isolates showed different kinetic constants.


CONCLUSIONS
The different enzymatic properties of laccases from various P. ostreatus strains should be considered for a potential industrial or environmental application.
Tinoco
R
R
Instituto de Biotecnologia UNAM, Cuernavaca, Morelos, Mexico.
Pickard
M A
MA
Vazquez-Duhalt
R
R
D000097:Acetonitriles / Q000494:pharmacology; D000363:Agaricales / Q000201:enzymology*; D002384:Catalysis; D004795:Enzyme Stability; D005656:Fungal Proteins / Q000737:chemistry / Q000378:metabolism; D006835:Hydrazones / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D042845:Laccase; D010088:Oxidoreductases / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D020076:Pleurotus / Q000201:enzymology*; D013696:Temperature
D000097:Acetonitriles; D005656:Fungal Proteins; D006835:Hydrazones; C009370:syringaldazine; D010088:Oxidoreductases; D042845:Laccase; C032159:acetonitrile