title: Comparison of salt effects on the reactions of acetylcholinesterase with cationic and anionic inhibitors.
abstract: The influence of inorganic salts on the inhibition of acetylcholinesterase by charged organophosphorous inhibitors has been studied. It has been shown that the salt effect on the reaction of acetylcholinesterase with anionic bis(p-nitrophenyl) phosphate is determined by the influence of added salts on the activity coefficient of the inhibitor. In contrast to the salt effects on the reaction of acetylcholinesterase with cationic compounds, it does not include contribution from the enzyme charges. The smaller salt effect in the case of anionic inhibitor can be explained assuming that the anionic inhibitor does not form a non-covalent complex with the enzyme before the phosphorylation step of the reaction. Comparison of salt effects on the substrate turnover showed that in the case of cholinesterases from natural sources they are larger than in the case of enzymes expressed in recombinant cell clones. The enhanced salt effects may result from post-translational modification of the enzyme.
authors.affiliation: Laboratory of Bioorganic Chemistry, National Institute of Chemical Physics and Biophysics, Akadeemia tee 23, 12618, Tallinn, Estonia. touugu@kbfi.ee
authors.forename: V
authors.initials: V
authors.lastname: Tõugu
authors.forename: T
authors.initials: T
authors.lastname: Kesvatera
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism*; D000838:Anions; D002412:Cations; D002800:Cholinesterase Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D004546:Elapid Venoms / Q000201:enzymology; D055672:Static Electricity
chemical_list: D000838:Anions; D002412:Cations; D002800:Cholinesterase Inhibitors; D004546:Elapid Venoms; D000110:Acetylcholinesterase