Tetramethylpyrazine inhibits phytohemagglutinin-induced upregulation of ICAM-1 and LFA-1 mediated leukocyte adhesion.
The aim of this work was to study the effects of tetramethylpyrazine (TMP) on the interaction between lymphocyte function-associated antigen-1 (LFA-1) and intercellular adhesive molecule-1 (ICAM-1) by micropipette technique. A pair of cells, i.e., a human lymphocyte or a S KW-3 cell (human T cell leukemia), LFA-1 was expressed on which cellular surfaces, and a RBC coupled with ICAM-1, were the carriers for LFA-1 and ICAM-1. The adhesion probabilities of this pair of cells were mediated by specific interactions between ICAM-1 and LFA-1. Phytohemagglutinin (PHA) stimulation resulted in the significant increase in the adhesion probability compared to the resting lymphocyte. TMP treatment can inhibit such increase and even make the adhesion probability lower than the resting state. While the LFA-1 expression has not changed significantly with PHA stimulation or with TMP treatment, which indicated that TMP inhibiting effects was realized by a possible conformation change.
Zhao
H
H
Research Institute, China-Japan Friendship Hospital, Beijing. hong.zhao@hemato.u-nancy.fr
Zhu
C
C
Li
X
X
Dong
X
X
Zhuang
F
F
Wang
X
X
Stoltz
J F
JF
D000328:Adult; D002448:Cell Adhesion / Q000187:drug effects / Q000235:genetics; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004907:Erythrocyte Deformability; D005260:Female; D006801:Humans; D007091:Image Processing, Computer-Assisted; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D015458:Leukemia, T-Cell / Q000473:pathology; D008213:Lymphocyte Activation / Q000187:drug effects*; D016169:Lymphocyte Function-Associated Antigen-1 / Q000737:chemistry / Q000378:metabolism*; D008297:Male; D008846:Micromanipulation; D008875:Middle Aged; D010835:Phytohemagglutinins / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D011485:Protein Binding / Q000187:drug effects; D011487:Protein Conformation / Q000187:drug effects; D011719:Pyrazines / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D013601:T-Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism
D004365:Drugs, Chinese Herbal; D016169:Lymphocyte Function-Associated Antigen-1; D010835:Phytohemagglutinins; D011719:Pyrazines; D018799:Intercellular Adhesion Molecule-1; C017953:tetramethylpyrazine