title: Cell surface CD28 levels define four CD4+ T cell subsets: abnormal expression in rheumatoid arthritis.
abstract: CD28 is a costimulatory receptor expressed in most CD4(+) T cells. Despite the long-standing evidence for up- and downregulation of surface CD28 expression in vitro, and the key regulatory role assigned to the upregulation of CD28 counterreceptor [the CD152 (CTLA-4) molecule], in vivo CD28 induction has attracted little attention. We studied CD28 and CD152 expression and function in 33 rheumatoid arthritis (RA) patients, 20 clinically active and 13 inactive, and in 24 healthy donors. Four subsets of CD28(-), CD28(low), CD28(int), and CD28(high) peripheral blood human CD4(+) T cells were defined using three-color flow cytometry. The three CD28(+) subsets displayed a one-, two-, or threefold quantitative difference in their relative number of CD28 antibody binding sites, respectively (P < 0.01). RA patients, whether active or inactive, showed a distinct phenotype when compared to healthy donors: (i) the percentage of CD4(+)CD28(high) cells was increased twofold and the CD4(+)CD28(low) subset was reduced twofold (P < 0.01) and (ii) the CD4(+)CD28(high) cells from RA patients showed an in vivo activated phenotype, CD45RO(+)CD5(high)IL-2Ralpha(+) (P < 0.01). Active RA patients were different from inactive patients. They showed a twofold increase in mean CD28 expression (P < 0.05), whereas each of the CD28(+) subsets in the inactive RA patients showed reduced expression when compared to healthy donors. Notably, both active and inactive RA patients showed abnormal CD28 upregulation when T cells were activated in vitro with CD3 antibodies, but only inactive RA patients showed a hypoproliferative response to TCR/CD3 triggering when compared to healthy donors (P < 0.01). This defective proliferation was normalized by concurrent crosslinking with CD28 antibody. No differences were noted in the expression of CD152 or CD80, a CD28 and CD152 shared ligand. The disregulated in vivo expression of CD28 was related to the RA patients' disease activity and suggests that modulation of CD28 surface levels may be an additional mechanism to finely tune the delicate responsiveness/tolerance balance.
authors.affiliation: Laboratory of Immunology and Oncology, School of Medicine, University of Alcalá-Centro de Investigaciones Biológicas (CSIC) Associated Unit, Ctra. Madrid-Barcelona Km. 33, 28871 Alcalá de Henares, Madrid, Spain.
authors.forename: L I
authors.initials: LI
authors.lastname: Salazar-Fontana
authors.forename: E
authors.initials: E
authors.lastname: Sanz
authors.forename: I
authors.initials: I
authors.lastname: Mérida
authors.forename: A
authors.initials: A
authors.lastname: Zea
authors.forename: A
authors.initials: A
authors.lastname: Sanchez-Atrio
authors.forename: L
authors.initials: L
authors.lastname: Villa
authors.forename: C
authors.initials: C
authors.lastname: Martínez-A
authors.forename: A
authors.initials: A
authors.lastname: de la Hera
authors.forename: M
authors.initials: M
authors.lastname: Alvarez-Mon
mesh_terms: D001172:Arthritis, Rheumatoid / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D001483:Base Sequence; D018106:CD28 Antigens / Q000235:genetics / Q000378:metabolism*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000473:pathology; D016022:Case-Control Studies; D017931:DNA Primers / Q000235:genetics; D006801:Humans; D007108:Immune Tolerance; D066298:In Vitro Techniques; D007376:Interleukin-2 / Q000235:genetics; D017493:Leukocyte Common Antigens / Q000378:metabolism; D008213:Lymphocyte Activation; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015375:Receptors, Interleukin-2 / Q000378:metabolism; D013583:Synovial Membrane / Q000276:immunology / Q000473:pathology; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000473:pathology; D015854:Up-Regulation
chemical_list: D018106:CD28 Antigens; D017931:DNA Primers; D007376:Interleukin-2; D012333:RNA, Messenger; D015375:Receptors, Interleukin-2; D017493:Leukocyte Common Antigens