title: Impact of Mitomycin-C application time on the scleral Mitomycin-C concentration.
abstract: The aim of this study was to determine the effect of varying the application time of Mitomycin-C (MMC) on the scleral concentration of MMC. The sclerae of 14 human donor eyes were used for this study. The episcleral sides of the 4 scleral quadrants of each donor eye were exposed for 0.5, 1, 3 and 5 min to round, 8 mm-diameter sponges soaked with 50 microl of 0.2 mg/ml MMC. After 40-ml irrigation with saline, a central 8-mm diameter scleral disk was punched out, homogenized and analyzed with high performance liquid chromatography (HPLC). The scleral MMC concentrations (microg/g) after 0.5, 1, 3 and 5 min application times were 6.40 (+/-3.38), 9.02 (+/-2.40), 12.31 (+/-3.37), and 13.97 (+/-3.83). The differences of scleral MMC concentration in paired t-tests were statistically significant comparing 0.5 with 1 and 1 with 5 min application. However the effect was relatively small within the range of usual application times (1 to 5 min), and 64% of the MMC was delivered to the sclera within the first min.
authors.affiliation: Department of Ophthalmology, University of Vienna, Austria. clemens.vass@akh-wien.ac.at
authors.forename: C
authors.initials: C
authors.lastname: Vass
authors.forename: M
authors.initials: M
authors.lastname: Georgopoulos
authors.forename: I
authors.initials: I
authors.lastname: El Menyawi
authors.forename: S
authors.initials: S
authors.lastname: Radda
authors.forename: P
authors.initials: P
authors.lastname: Nimmerrichter
mesh_terms: D000704:Analysis of Variance; D000903:Antibiotics, Antineoplastic / Q000493:pharmacokinetics*; D006801:Humans; D016685:Mitomycin / Q000493:pharmacokinetics*; D012590:Sclera / Q000378:metabolism*; D013997:Time Factors; D014018:Tissue Distribution
chemical_list: D000903:Antibiotics, Antineoplastic; D016685:Mitomycin