title: Dermal benzene and trichloroethylene induce aneuploidy in immature hematopoietic subpopulations in vivo.
abstract: Accumulation of genetic damage in long-lived cell populations with proliferative capacity is implicated in tumorigenesis. Hematopoietic stem cells (hsc) maintain lifetime hematopoiesis, and recent studies demonstrate that hsc in leukemic patients are cytogenetically aberrant. We postulated that exposure to agents associated with increased leukemia risk would induce genomic changes in cells in the hsc compartment. Aneusomy involving chromosomes 2 and 11 in sorted hsc (Lin(-)c-kit(+)Sca-1(+)) and maturing lymphoid and myeloid cells from mice that received topical doses of benzene (bz) or trichloroethylene (TCE) was quantified using fluorescence in situ hybridization. Six days after bz or TCE exposure, aneuploid cells in the hsc compartment increase four- to eightfold in a dose- and schedule-independent manner. Aneuploid lymphoid and myeloid cells from bz- and TCE-treated mice approximate controls, except after repeated benzene exposures. Aneuploid cells are more frequent in the hsc compartment than in mature hematopoietic subpopulations. Hematotoxicity was also quantified in bz- and TCE-exposed hematopoietic subpopulations using two colony-forming assays: CFU-GM (colony-forming units/granulocyte-macrophage progenitors) and CAFC (cobblestone area-forming cells). Data indicate that bz is transiently cytotoxic (< or =1 week) to hsc subpopulations, and induces more persistent toxicity (>2 weeks) in maturing, committed progenitor subpopulations. TCE is not hematotoxic at the doses applied. In conclusion, we provide direct evidence for induction of aneuploidy in cells in the hsc compartment by topical exposure to bz and TCE. Disruption of genomic integrity and/or toxicity in hsc subpopulations may be one step in leukemic progression.
authors.affiliation: Cancer Center, University of California, San Francisco, California 94143-0808, USA.
authors.forename: C R
authors.initials: CR
authors.lastname: Giver
authors.forename: R
authors.initials: R
authors.lastname: Wong
authors.forename: D H
authors.initials: DH
authors.lastname: Moore
authors.forename: M G
authors.initials: MG
authors.lastname: Pallavicini
mesh_terms: D000279:Administration, Cutaneous; D000782:Aneuploidy; D000818:Animals; D000950:Antigens, Ly / Q000378:metabolism; D001554:Benzene / Q000008:administration & dosage / Q000633:toxicity*; D001854:Bone Marrow Cells / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D019070:Cell Lineage; D005260:Female; D006412:Hematopoietic Stem Cells / Q000187:drug effects* / Q000502:physiology; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D019009:Proto-Oncogene Proteins c-kit / Q000378:metabolism; D014241:Trichloroethylene / Q000008:administration & dosage / Q000633:toxicity*
chemical_list: D000950:Antigens, Ly; C074650:Ly6a protein, mouse; D008565:Membrane Proteins; D014241:Trichloroethylene; D019009:Proto-Oncogene Proteins c-kit; D001554:Benzene