Antibody levels in goats fed Toxoplasma gondii oocysts.
Outbred goats were fed 10(5) Toxoplasma gondii oocysts and were monitored twice weekly for 8 wk for rectal temperature, clinical signs, parasitemia, and antibody levels by indirect fluorescence antibody test (IFAT), latex agglutination test (LAT), and indirect enzyme-linked immunosorbent assay (ELISA). After 8 wk, all goats were killed, and samples of heart, skeletal muscle, brain, lymph nodes, kidneys, and liver were bioassayed in mice. Anorexia, fever, and lethargy were observed from day 3 to day 7 postinfection (PI). Parasitemia was detected by bioassay in 50% of infected goats from day 7 to day 14 PI. Viable T. gondii organisms were isolated from all infected goats. Antibodies to T. gondii were detected in some animals on day 10 PI by IFAT and LAT and on day 14 PI by ELISA. The infected goats were seropositive on day 17 PI.
Nishi
S M
SM
Department of Preventive Veterinary Medicine and Animal Health, Faculty of Veterinary Medicine,University of São Paulo, SP, Brazil.
Kasai
N
N
Gennari
S M
SM
D000818:Animals; D000855:Anorexia / Q000150:complications / Q000662:veterinary; D000913:Antibodies, Protozoan / Q000032:analysis*; D001831:Body Temperature; D002415:Cats; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D019084:Fluorescent Antibody Technique, Indirect / Q000662:veterinary; D015511:Goat Diseases / Q000276:immunology*; D006041:Goats; D007841:Latex Fixation Tests / Q000662:veterinary; D051379:Mice; D018512:Parasitemia / Q000150:complications / Q000662:veterinary; D014122:Toxoplasma / Q000276:immunology*; D014124:Toxoplasmosis, Animal / Q000150:complications / Q000276:immunology*
D000913:Antibodies, Protozoan