Isolation and characterization of a human thiamine pyrophosphokinase cDNA.
A human thiamine pyrophosphokinase cDNA clone (hTPK1) was isolated and sequenced. When the intact hTPK1 open reading frame was expressed as a histidine-tag fusion protein in Escherichia coli, marked enzyme activity was detected in the bacterial cells. The hTPK1 mRNA was widely expressed in various human tissues at a very low level, and the mRNA content in cultured fibroblasts was unaffected by the thiamine concentration of the medium. The chromosome localization of the hTPK1 gene was assigned to 7q34.
Nosaka
K
K
Department of Chemistry, Kyoto Prefectural University of Medicine, Kita-ku, Kyoto 603-8334, Japan. knosaka@basic.kpu-m.ac.jp
Onozuka
M
M
Kakazu
N
N
Hibi
S
S
Nishimura
H
H
Nishino
H
H
Abe
T
T
D000595:Amino Acid Sequence; D000749:Anemia, Megaloblastic / Q000201:enzymology / Q000235:genetics; D015152:Blotting, Northern; D002897:Chromosomes, Human, Pair 7; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005347:Fibroblasts / Q000201:enzymology; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007668:Kidney / Q000201:enzymology; D007962:Leukocytes / Q000201:enzymology; D008969:Molecular Sequence Data; D009206:Myocardium / Q000201:enzymology; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D016415:Sequence Alignment; D013836:Thiamin Pyrophosphokinase / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*
D018076:DNA, Complementary; D012333:RNA, Messenger; D013836:Thiamin Pyrophosphokinase