[Characteristic of mutants of Pseudomonas putida IPM-36 recombinant strains, selected by anticipating growth on a media containing an inducer of cry3A gene expression].
Induction of the expression of the delta-endotoxin gene from Bacillus thuringiensis var. tenebrionis in the recombinant strain Pseudomonas putida IPM-36 negatively affected the viability and the growth rate of the culture. In order to optimize the insecticide production by the recombinant strain, mutant clones exhibiting anticipating growth on an inducer-containing medium were selected and studied. These clones differed in such aspects as the localization of mutations (either in plasmid pBTN11, carrying the cry3A gene, or in the chromosome), growth rate, or the level of delta-endotoxin synthesis after induction. Several mutants obtained proved much superior to P. putida IPM-36 in their structural and segregation stability, although they were as efficient as the original strain with respect to the production of the insecticide (protei Cry3A).
Koretskaia
N G
NG
State Research Center for Applied Microbiology, Obolensk, Moscow oblast, 142279 Russia.
Svetoch
O E
OE
Loseva
O I
OI
Dobritsa
A P
AP
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000235:genetics*; D001427:Bacterial Toxins; D003470:Culture Media; D004731:Endotoxins / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D006460:Hemolysin Proteins; D009154:Mutation; D010572:Pest Control, Biological; D016958:Pseudomonas putida / Q000235:genetics* / Q000254:growth & development*; D011995:Recombination, Genetic
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D003470:Culture Media; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis