Determination of growth hormone-releasing hexapeptide by reversed-phase high-performance liquid chromatography with electrochemical detection.
A novel HPLC method with electrochemical detection is described for the determination of a growth-hormone-releasing hexapeptide (GHRP-6). HPLC conditions, such as the column, mobile phase, and oxidation potential, were optimized for sensitivity and selectivity of analysis. GHRP-6 was separated on a reversed-phase CN column with 37% acetonitrile in 100 mM phosphate buffer (pH 7.0) as the mobile phase. The optimum electrochemical oxidation signal was obtained at 0.85 V vs. Ag/AgCl in a glassy carbon working electrode due to two electroactive tryptophans and a histidine residue. Solid-phase extraction using octadecyl cartridges was optimized for sample cleanup of GHRP-6 from serum samples and the method was successfully applied over the concentration range of 5 to 100 ng/ml of analyte. reserved.
Choi
S J
SJ
Bioanalysis Laboratory, College of Pharmacy, Wonkwang University, Iksan, South Korea.
Lee
H Y
HY
Kim
S B
SB
Kim
J H
JH
Lee
S S
SS
Yoo
S D
SD
Lee
K C
KC
Lee
H S
HS
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004563:Electrochemistry; D006801:Humans; D009842:Oligopeptides / Q000097:blood* / Q000378:metabolism; D010084:Oxidation-Reduction; D015203:Reproducibility of Results
D009842:Oligopeptides; C041048:growth hormone releasing hexapeptide