Primary study of vascular endothelial growth factor immunohistochemical staining in the diagnosis of early acute myocardial ischemia.
The expression of vascular endothelial growth factor (VEGF) in the model of rat early acute myocardial ischemia was studied by Strept-Avidin-Biotin-Peroxidase Complex (SABC) immunohistochemical staining. After ligating the anterior descending branch of the left coronary artery, an initial rapid (30min) positive expression of VEGF in myocardial ischemic areas was observed, the intensity of positive expression of VEGF increased with the continuation of myocardial ischemia. After 5h infarction, the strongly positive myocytes of SABC-VEGF staining were predominantly limited to perimyocardial infarction areas. No positive expression of VEGF was found in the control group. These findings suggested that SABC-VEGF method could give a sensitive, specific, simple and objective morphologic evidence to the diagnosis of sudden cardiac death caused by acute early myocardial ischemia.
Xu
X H
XH
Department of Forensic Pathology, Shantou University Medical College, 515031, Shantou, PR China.
Chen
J G
JG
Zhu
J Z
JZ
D000818:Animals; D016757:Death, Sudden, Cardiac; D016228:Endothelial Growth Factors / Q000302:isolation & purification*; D005260:Female; D005554:Forensic Medicine; D006801:Humans; D007202:Indicators and Reagents; D008222:Lymphokines / Q000302:isolation & purification*; D008297:Male; D017202:Myocardial Ischemia / Q000175:diagnosis* / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D019809:Streptavidin; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
D016228:Endothelial Growth Factors; D007202:Indicators and Reagents; D008222:Lymphokines; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; D019809:Streptavidin