Effect of IFN-gamma receptor gene deletion on vaccinia virus virulence.
Two vaccina virus (VV) strains, WR and Praha, were selected for a study undertaken to determine whether the virus-encoded interferon-gamma receptor (IFN-gamma R) plays any role in virus virulence. Both of the viruses expressed the B8R gene coding for IFN-gamma R in infected cell cultures. The nucleotide sequence of the Praha virus B8R gene was determined, and, when compared with the published sequence of the WR virus, it only displayed one silent nucleotide substitution. Mutants of the WR and Praha viruses with deleted B8R gene were constructed. In rabbits, skin lesions produced by the WR B8R-deleted mutants were smaller and tended to disappear earlier than those caused by wild-type WR virus. Similar results were obtained with both independently prepared WR B8R-deleted mutants. These data strongly suggested that the product of B8R gene did play a role in virus virulence. A similar comparison of the wild-type Praha virus and its mutant could not be done because of the very low virulence of the parental virus for rabbits.
Sroller
V
V
Institute of Hematology and Blood Transfusion, Praha, Czech Republic.
Ludvíková
V
V
Maresová
L
L
Hainz
P
P
Nĕmecková
S
S
D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D017931:DNA Primers; D017353:Gene Deletion; D006801:Humans; D007371:Interferon-gamma / Q000502:physiology; D051379:Mice; D016133:Polymerase Chain Reaction; D011817:Rabbits; D017471:Receptors, Interferon / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D014615:Vaccinia / Q000276:immunology; D014616:Vaccinia virus / Q000472:pathogenicity* / Q000502:physiology*; D014774:Virulence; D000096743:Interferon gamma Receptor
D017931:DNA Primers; D007371:Interferon-gamma; D017471:Receptors, Interferon; D000096743:Interferon gamma Receptor