title: Pathophysiological significance of in vivo ESR signal decay in brain damage caused by X-irradiation. Radiation effect on nitroxyl decay of a lipophilic spin probe in the head region.
abstract: X-irradiation of mice decreased the decay rate of the in vivo ESR signal in the head region to 75% of the control when 3-methoxycarbonyl-2,2,5,5-tetramethylpyrrolidine-1-yloxy (MCPROXYL), a lipophilic and blood-brain barrier-permeable spin probe, was used. We attempted to identify the specific factor responsible for the decrease in the signal decay rate caused by X-irradiation. The signal decay of MCPROXYL in the head region depends on the following three factors: (1) blood concentration of MCPROXYL, (2) reduction to the corresponding hydroxylamine in the brain tissue, and (3) effusion of MCPROXYL from the brain tissue. Irradiation at 15 Gy did not significantly change the rate of decrease of blood concentration of MCPROXYL at 1 h post-irradiation. The reducing activity of the brain homogenate was not changed by the X-irradiation (15 Gy). The contents of MCPROXYL and its hydroxylamine derivative in the brain of 15 Gy-irradiated mice remained higher than in non-irradiated mice. These findings suggest that the effect of X-irradiation observed by in vivo ESR is attributable not to the redox reaction of MCPROXYL in the brain but to the change of the efflux rate of the MCPROXYL from the brain.
authors.affiliation: Department of Bioregulation Research, National Institute of Radiological Sciences, Chiba, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Miura
authors.forename: K
authors.initials: K
authors.lastname: Anzai
authors.forename: J I
authors.initials: JI
authors.lastname: Ueda
authors.forename: T
authors.initials: T
authors.lastname: Ozawa
mesh_terms: D000818:Animals; D001930:Brain Injuries / Q000503:physiopathology*; D003497:Cyclic N-Oxides; D004578:Electron Spin Resonance Spectroscopy; D005260:Female; D066298:In Vitro Techniques; D051379:Mice; D009589:Nitrogen Oxides / Q000737:chemistry / Q000528:radiation effects; D011759:Pyrrolidines / Q000737:chemistry / Q000528:radiation effects; D011833:Radiation Injuries, Experimental / Q000503:physiopathology*; D013113:Spin Labels
chemical_list: D003497:Cyclic N-Oxides; C109806:MCPROXYL; D009589:Nitrogen Oxides; D011759:Pyrrolidines; D013113:Spin Labels; C039900:nitroxyl