Direct somatic embryogenesis and plantlet regeneration from leaf explants of niger, Guizotia abyssinica (L.f.) Cass.
Somatic embryogenesis was induced in niger (Guizotia abyssinica (L.f.) Cass.) using a simple one step method. Leaf explants were cultured on MS medium with 2,4-D and BAP/Kn individually and in combination. Somatic embryogenesis occurred directly without an intervening callus proliferation phase from subepidermal regions of leaf explants on MS medium supplemented with 2,4-D (2.0 and 5.0 microM) plus Kn/BAP (1.0 and 2.0 microM). Regenerated somatic embryos were successfully grown into whole plants.
Kumar
H G
HG
Plant Tissue Culture Laboratory, Department of Botany, Karnatak University, Dharwad 580003, India.
Murthy
H N
HN
Jadimath
V G
VG
Sheelavantmath
S S
SS
Pyati
A N
AN
Ravishankar
B V
BV
D019659:Asteraceae / Q000166:cytology / Q000196:embryology* / Q000254:growth & development; D002455:Cell Division; D018515:Plant Leaves / Q000254:growth & development*; D012038:Regeneration; D012639:Seeds / Q000254:growth & development