title: Characterization of the genomic Xist locus in rodents reveals conservation of overall gene structure and tandem repeats but rapid evolution of unique sequence.
abstract: The Xist locus plays a central role in the regulation of X chromosome inactivation in mammals, although its exact mode of action remains to be elucidated. Evolutionary studies are important in identifying conserved genomic regions and defining their possible function. Here we report cloning, sequence analysis, and detailed characterization of the Xist gene from four closely related species of common vole (field mouse), Microtus arvalis. Our analysis reveals that there is overall conservation of Xist gene structure both between different vole species and relative to mouse and human Xist/XIST. Within transcribed sequence, there is significant conservation over five short regions of unique sequence and also over Xist-specific tandem repeats. The majority of unique sequences, however, are evolving at an unexpectedly high rate. This is also evident from analysis of flanking sequences, which reveals a very high rate of rearrangement and invasion of dispersed repeats. We discuss these results in the context of Xist gene function and evolution.
authors.affiliation: Institute of Cytology and Genetics, Russian Academy of Sciences, Siberian Department, Novosibirsk 630090, Russia. tatyana.nesterova@csc.mrc.ac.uk
authors.forename: T B
authors.initials: TB
authors.lastname: Nesterova
authors.forename: S Y
authors.initials: SY
authors.lastname: Slobodyanyuk
authors.forename: E A
authors.initials: EA
authors.lastname: Elisaphenko
authors.forename: A I
authors.initials: AI
authors.lastname: Shevchenko
authors.forename: C
authors.initials: C
authors.lastname: Johnston
authors.forename: M E
authors.initials: ME
authors.lastname: Pavlova
authors.forename: I B
authors.initials: IB
authors.lastname: Rogozin
authors.forename: N N
authors.initials: NN
authors.lastname: Kolesnikov
authors.forename: N
authors.initials: N
authors.lastname: Brockdorff
authors.forename: S M
authors.initials: SM
authors.lastname: Zakian
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D020121:5' Untranslated Regions / Q000235:genetics; D000818:Animals; D000835:Animals, Wild / Q000235:genetics; D003411:Arvicolinae / Q000235:genetics; D001483:Base Sequence / Q000235:genetics; D002478:Cells, Cultured; D002874:Chromosome Mapping; D017124:Conserved Sequence / Q000235:genetics*; D004247:DNA / Q000032:analysis*; D019143:Evolution, Molecular; D005260:Female; D005796:Genes; D005819:Genetic Markers; D006801:Humans; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D062085:RNA, Long Noncoding; D022661:RNA, Untranslated / Q000235:genetics*; D020080:Tandem Repeat Sequences / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic; D014960:X Chromosome / Q000235:genetics
chemical_list: D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D005819:Genetic Markers; D062085:RNA, Long Noncoding; D022661:RNA, Untranslated; D014157:Transcription Factors; C077885:XIST non-coding RNA; D004247:DNA