Effect of anti-digoxin antiserum on endoxin and membrane ATPase activity in hypoxia-reoxygenation induced myocardial injury.
OBJECTIVE
To evaluate the protective effect of anti-digoxin antiserum on hypoxia-reoxygenation induced injured myocardium and its mechanism.


METHODS
Anti-digoxin antiserum of different concentrations was used, its effect on endoxin and ATPase activity in cell membrane in hypoxia-reoxygenation myocardium model was observed.


RESULTS
The level of endoxin was remarkably higher, ATPase activities in cell membrane were remarkably lower in hypoxic group and hypoxia-reoxygenation injury group than those of normal group; anti-digoxin antiserum could resume ATPase activity in a concentration-dependent manner.


CONCLUSIONS
Rise of endoxin was the molecular biological basis of myocardial damage during myocardial hypoxia-reoxygenation. Anti-digoxin antiserum had lessened myocardial injury and had a protective effect on hypoxia-reoxygenation myocardium by antagonizing effect of endoxin.
Ke
Y S
YS
Yijishan Hospital, Wannan Medical College, Wuhu 241001, China. editorys@mail.ahwhptt.net.cn
Liu
Z F
ZF
Yang
H
H
Yang
T
T
Huang
J S
JS
Rui
S B
SB
Cheng
G H
GH
Wang
Y X
YX
D000818:Animals; D000252:Calcium-Transporting ATPases / Q000378:metabolism*; D002298:Cardenolides; D015687:Cell Hypoxia; D002462:Cell Membrane / Q000378:metabolism; D004077:Digoxin / Q000276:immunology*; D004791:Enzyme Inhibitors / Q000378:metabolism; D005260:Female; D007106:Immune Sera / Q000494:pharmacology*; D008297:Male; D015428:Myocardial Reperfusion Injury / Q000378:metabolism; D009206:Myocardium / Q000166:cytology*; D011817:Rabbits; D012503:Saponins / Q000378:metabolism*
D002298:Cardenolides; D004791:Enzyme Inhibitors; D007106:Immune Sera; D012503:Saponins; C034504:digoxin-like factors; D004077:Digoxin; D000252:Calcium-Transporting ATPases