The celA gene, encoding a glycosyl hydrolase family 3 beta-glucosidase in Azospirillum irakense, is required for optimal growth on cellobiosides.
The CelA beta-glucosidase of Azospirillum irakense, belonging to glycosyl hydrolase family 3 (GHF3), preferentially hydrolyzes cellobiose and releases glucose units from the C(3), C(4), and C(5) oligosaccharides. The growth of a DeltacelA mutant on these cellobiosides was affected. In A. irakense, the GHF3 beta-glucosidases appear to be functional alternatives for the GHF1 beta-glucosidases in the assimilation of beta-glucosides by other bacteria.
Faure
D
D
F. A. Janssens Laboratory of Genetics, Katholieke Universiteit Leuven, K. Mercierlaan 92, B-3001 Heverlee, Belgium.
Henrissat
B
B
Ptacek
D
D
Bekri
M A
MA
Vanderleyden
J
J
D000595:Amino Acid Sequence; D016949:Azospirillum / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development*; D002475:Cellobiose / Q000378:metabolism; D002480:Cellulase / Q000235:genetics* / Q000378:metabolism; D003470:Culture Media; D005960:Glucosides / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000145:classification* / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D010641:Phenotype; D017422:Sequence Analysis, DNA; D001617:beta-Glucosidase / Q000235:genetics* / Q000378:metabolism*
D003470:Culture Media; D005960:Glucosides; D002475:Cellobiose; D006026:Glycoside Hydrolases; D001617:beta-Glucosidase; C074775:CelA endoglucanase; D002480:Cellulase