Deletion of the cytoplasmic tail of the fusion protein of the paramyxovirus simian virus 5 affects fusion pore enlargement.
The fusion (F) protein of the paramxyovirus simian parainfluenza virus 5 (SV5) promotes virus-cell and cell-cell membrane fusion. Previous work had indicated that removal of the SV5 F protein cytoplasmic tail (F Tail- or FDelta19) caused a block in fusion promotion at the hemifusion stage. Further examination has shown that although the F Tail- mutant is severely debilitated in promotion of fusion as measured by using two reporter gene assays and is debilitated in the formation of syncytia relative to the wild-type F protein, the F Tail- mutant is capable of promoting the transfer of small aqueous dyes. These data indicate that F Tail- is fully capable of promoting formation of small fusion pores. However, enlargement of fusion pores is debilitated, suggesting that either the cytoplasmic tail of the F protein plays a direct role in pore expansion or that it interacts with other components which control pore growth.
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Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, Illinois 60208-3500, USA.
Lamb
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D000818:Animals; D001692:Biological Transport; D002460:Cell Line; D004396:Coloring Agents; D004910:Erythrocyte Membrane / Q000378:metabolism / Q000473:pathology; D004912:Erythrocytes / Q000378:metabolism; D015726:Giant Cells; D006801:Humans; D050356:Lipid Metabolism; D008561:Membrane Fusion; D009154:Mutation; D019213:Rubulavirus / Q000235:genetics*; D014162:Transfection; D014760:Viral Fusion Proteins / Q000032:analysis / Q000235:genetics* / Q000378:metabolism
D004396:Coloring Agents; D014760:Viral Fusion Proteins