Comparative study of blood group-recognizing lectins toward ABO blood group antigens on neoglycoproteins, glycoproteins and complex-type oligosaccharides.
Binding specificities of ABO blood group-recognizing lectins toward blood group antigens on neoglycoproteins, glycoproteins and complex-type oligosaccharides were studied by lectin-blotting analysis, enzyme linked immunosorbent assay and lectin-conjugated agarose column chromatography. Human serum albumin conjugated with A- and B-trisaccharides was clearly recognized by Helix pomatia (HPA), Phaseolus lunatus, Dolichos biflorus agglutinins, and Griffonia simplicifolia I agglutinin B(4), respectively. Almost the same results were obtained for human group A and B ovarian cyst and A-active hog gastric mucins, but Glycine max agglutinin only reacted to the group A hog mucin. When human plasma von Willebrand factor (vWF), having Asn-linked blood group antigens, was tested, HPA was highly sensitive to blood group A antigen on the vWF. Ulex europaeus agglutinin I (UEA-I) preferentially bound to the vWF from blood group O plasma. Within the GalNAc-recognizing lectins examined, a biantennary complex-type oligosaccharide having the blood group A structure retarded on an HPA-agarose column, and the affinity was diminished after digestion with alpha-N-acetylgalactosaminidase. This product bound to UEA-I agarose column. These results indicate that HPA and UEA-I are most sensitive for detection of glycoproteins possessing small amounts of blood group A and H antigens and also useful for fractionation of complex-type oligosaccharides with blood group A and H antigens, respectively.
Matsui
T
T
Division of Biomedical Polymer Science, Institute for Comprehensive Medical Science, Fujita Health Univeristy, Toyoake, Aichi, Japan. tmatsui@fujita-hu.ac.jp
Hamako
J
J
Ozeki
Y
Y
Titani
K
K
D000017:ABO Blood-Group System / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D000328:Adult; D000818:Animals; D002240:Carbohydrate Sequence; D006023:Glycoproteins / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D037102:Lectins / Q000378:metabolism*; D008969:Molecular Sequence Data; D009077:Mucins / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D009844:Oligosaccharides / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D014841:von Willebrand Factor / Q000737:chemistry / Q000276:immunology / Q000378:metabolism
D000017:ABO Blood-Group System; D006023:Glycoproteins; D037102:Lectins; D009077:Mucins; D009844:Oligosaccharides; D014841:von Willebrand Factor