Occurrence of Listeria species in retail poultry meat and comparison of a cultural/immunoassay for their detection.
The prevalence of Listeria spp. on the skin of a hundred fresh chicken carcasses purchased from 20 retail stores in León was investigated using the routine test procedure recommended by the United States Department of Agriculture (USDA). PALCAM and Modified Oxford agar (MOX) were used for isolation. Listeria spp., Listeria monocytogenes, L. innocua, L. welshimeri, L. grayi and L. ivanovii were present in 95%, 32%, 66%, 7%, 4% and 2% of the samples, respectively. Next, an immunoassay test (Listeria Rapid Test; Oxoid, Unipath) and the routine test procedure (USDA) were compared for their ability to detect Listeria spp. on 40 chicken carcasses. When we used MOX for the isolations, the sensitivity of the immunoassay test was 100% and the specificity 85.7%. When we used PALCAM medium the sensitivity and specificity of the test was 94.29% and 80%, respectively.
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Department of Food Hygiene and Food Technology, Faculty of Veterinary, University of León, Spain. dhtrcg@unileon.es
Alonso-Calleja
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Moreno
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García-Fernandez
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D000818:Animals; D002645:Chickens; D015169:Colony Count, Microbial / Q000379:methods; D005516:Food Microbiology; D007118:Immunoassay / Q000379:methods; D008087:Listeria / Q000302:isolation & purification*; D011200:Poultry / Q000382:microbiology*; D012680:Sensitivity and Specificity