PCR detection of Helicobacter pylori in string-absorbed gastric juice.
Molecular methods for detection of Helicobacter pylori infection have been shown to be highly sensitive in gastric biopsies and cultures. The objective of this work was to compare PCR detection of H. pylori DNA in string-absorbed gastric juice and in gastric biopsies. The study was performed in 47 dyspeptic adult patients undergoing endoscopy, and infection was detected by amplification of a segment of H. pylori ureA gene. Of the 29 patients positive in biopsy analysis, 23 (79%) were also positive in the gastric string. PCR analysis of gastric strings is a sensitive and safe procedure to detect H. pylori when endoscopy is not indicated, and may be of great clinical and epidemiological usefulness in determining effectiveness of eradication therapies, typing virulence genes and detecting antibiotic resistance mutations.
Domínguez-Bello
M G
MG
Instituto Venezolano de Investigaciones Cientificas, CBB, Caracas, Venezuela. mgdbello@ivic.ivic.ve
Cienfuentes
C
C
Romero
R
R
García
P
P
Gómez
I
I
Mago
V
V
Reyes
N
N
Gueneau de Novoa
P
P
D000328:Adult; D001706:Biopsy; D005750:Gastric Juice / Q000382:microbiology*; D016481:Helicobacter Infections / Q000175:diagnosis*; D016480:Helicobacter pylori / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D016133:Polymerase Chain Reaction; D011706:Pyloric Antrum / Q000382:microbiology; D012680:Sensitivity and Specificity; D013048:Specimen Handling; D014510:Urease / Q000235:genetics
D014510:Urease