Anticancer treatment of endostatin gene therapy by targeting tumor neovasculature in C57/BL mice.
Antiangiogenesis strategy has been widely recognized as a viable approach to fight cancer. Considering the high cost and inconvenience of protein therapy of endostatin (ES), which is a potent antiangiogenic protein, we attempted to explore the inhibitory effect of ES gene therapy on tumor growth and metastasis. In this experiment, Lewis lung carcinoma (LLC)-bearing C57/BL mice were used to evaluate the antitumor effect of ES gene therapy and its impairment of tumor neovasculature. The data showed that the ectopic ES in circulation expressed by intramuscular administration of formulated ES-encoding plasmid DNA significantly suppressed primary tumor growth and lung metastasis in LLC-bearing C57/BL mice. Hence, our results demonstrated the inhibitory effect of ES gene therapy on angiogenesis-dependent tumor growth and metastasis.
Jia
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S
Institute of Microcirculation, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing. xiurj@cdm.imicam.ac.cn
Zhu
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F
Li
H
H
He
F
F
Xiu
R
R
D020533:Angiogenesis Inhibitors / Q000235:genetics*; D000818:Animals; D019556:COS Cells; D018827:Carcinoma, Lewis Lung / Q000098:blood supply / Q000628:therapy*; D002455:Cell Division; D002522:Chlorocebus aethiops; D003094:Collagen / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D004354:Drug Screening Assays, Antitumor; D043169:Endostatins; D004730:Endothelium, Vascular / Q000166:cytology; D005260:Female; D015316:Genetic Therapy; D005822:Genetic Vectors / Q000008:administration & dosage / Q000627:therapeutic use*; D006801:Humans; D007273:Injections, Intramuscular; D008175:Lung Neoplasms / Q000098:blood supply / Q000556:secondary*; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009362:Neoplasm Metastasis; D009389:Neovascularization, Pathologic / Q000628:therapy*; D010446:Peptide Fragments / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000235:genetics; D014162:Transfection
D020533:Angiogenesis Inhibitors; D018076:DNA, Complementary; D043169:Endostatins; D010446:Peptide Fragments; D011993:Recombinant Fusion Proteins; D003094:Collagen