title: Effect of genistein analogs on oxygen radical production in leukocytes stimulated by unopsonized zymosan.
abstract: Effects of genistein analogs on oxygen radical production have been analyzed in human neutrophils, human monocytes or murine macrophages Raw264.7 stimulated with unopsonized zymosan by lucigenin- and luminol-enhanced chemiluminescence assays. Genistein exhibited IC50 values of 10.7-11.5 microM on the oxygen radical production in human neutrophils, 10.9-11.0 microM in human monocytes, and 14.8-27.3 microM in Raw264.7 cells. Orobol, a genistein analog with an additional hydroxy group at the 3' position, exhibited IC50 values of 3.0-3.3 microM on the oxygen radical production in human neutrophils, 2.8-3.1 microM in human monocytes, and 1.5-3.9 microM in Raw264.7 cells. Genistin and sophoricoside are genistein glycosides with a glucose moiety at 7 or 4' position, respectively. The genistein glycosides exhibited 23-37% inhibitory effects at 100 microM on the oxygen radical production.
authors.forename: J
authors.initials: J
authors.lastname: Yun
authors.forename: S R
authors.initials: SR
authors.lastname: Oh
authors.forename: H K
authors.initials: HK
authors.lastname: Lee
authors.forename: I M
authors.initials: IM
authors.lastname: Chang
authors.forename: K
authors.initials: K
authors.lastname: Takatsu
authors.forename: Y P
authors.initials: YP
authors.lastname: Yun
authors.forename: K R
authors.initials: KR
authors.lastname: Min
authors.forename: Y
authors.initials: Y
authors.lastname: Kim
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D019833:Genistein / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D007962:Leukocytes / Q000187:drug effects*; D008163:Luminescent Measurements; D008264:Macrophages / Q000187:drug effects; D051379:Mice; D009000:Monocytes / Q000187:drug effects; D009504:Neutrophils / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D013481:Superoxides / Q000378:metabolism*; D015054:Zymosan
chemical_list: D013481:Superoxides; D015054:Zymosan; D019833:Genistein