[Continuously perfused cultivation of genetically-engineered CHO cells producing prothrombin in a modified Super-Spinner].
A Super-Spinner was Modified by mounting a stainless steel filter(pore size 75 microns) to the impeller shaft to retain cells while fresh nutrient is perfused. Using Macroporous microcarrier Cytopore 1, continuously perfused cultivation of a recombinant CHO cell line, CHO2DS producing prothrombin was performed with the perfusion of a protein-free medium DF6S. The cell retention rate was more than 90% during the 24 days continuously perfused cultivation. The viable cell density of CHO2DS and prothrombin concentration reached 4.62 x 10(6)(cells.m/L) and 11.3(mg/L) respectively after 9 days culture.
Chen
Z L
ZL
Institute of Biotechnology, Academy of Military Medical Sciences, Beijing 100071, China. chenzl@nic.bmi.ac.cn
Iding
K
K
Lütkemeyer
D
D
Lehmann
J
J
D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D005818:Genetic Engineering; D010477:Perfusion; D011516:Prothrombin / Q000096:biosynthesis*; D011994:Recombinant Proteins / Q000096:biosynthesis*
D011994:Recombinant Proteins; D011516:Prothrombin