Lamellipodia in invasion.
In vivo imaging of GFP-labeled metastatic tumor cells reveals cell orientation towards blood vessels. Orientation of tumor cells during chemotactic responses to ligands such as EGF begins with lamellipod extension. Evaluation of some of the downstream events in lamellipod extension indicates: (1) plasma membrane distribution of the EGF receptor is uniform but internalized receptor accumulates on the side of the cell closest to the source of EGF; (2) the alpha p110 isoform of PI-3 kinase is required; and (3) protrusion of the lamellipod relies upon the combined actions of the Arp2/3 complex and cofilin for generation of filamentous actin.
Condeelis
J S
JS
Department of Anatomy and Structural Biology and the Intravital Imaging Program, Analytical Imaging Facility, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
Wyckoff
J B
JB
Bailly
M
M
Pestell
R
R
Lawrence
D
D
Backer
J
J
Segall
J E
JE
D051377:Actin-Related Protein 2; D000199:Actins / Q000378:metabolism; D000818:Animals; D002465:Cell Movement / Q000502:physiology*; D002633:Chemotaxis; D003598:Cytoskeletal Proteins; D004730:Endothelium, Vascular / Q000502:physiology*; D066246:ErbB Receptors / Q000502:physiology*; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000378:metabolism; D009361:Neoplasm Invasiveness; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D011485:Protein Binding; D011554:Pseudopodia / Q000378:metabolism*; D014162:Transfection
D051377:Actin-Related Protein 2; D000199:Actins; D003598:Cytoskeletal Proteins; D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins; D019869:Phosphatidylinositol 3-Kinases; D066246:ErbB Receptors