title: Assessment of skeletal muscle ventricle tissue blood flow using positron emission tomography.
abstract: In this pilot study, we assessed the feasibility of using positron emission tomography (PET) imaging for in vivo measurement of skeletal muscle ventricle (SMV) tissue blood flow. In 4 dogs, with SMVs prepared from their latissimus dorsi muscle, we quantified SMV tissue blood flow by PET and related it to the tissue flow measured by radiolabeled microspheres under similar physiologic conditions. The tissue blood flow was estimated in SMVs wrapped around a mandrel (not in circulation) at rest and during SMV stimulation (30 and 90 contraction-cycles/min). SMV tissue perfusion was heterogeneous, especially during SMV contraction. Furthermore, there was a linear relationship between SMV tissue flows estimated by PET and those measured by microspheres. We conclude that in vivo imaging of SMV is feasible by PET. Quantification of SMV tissue blood flow by PET has promise as a means of assessing changes in blood flow, but further technical progress needs to be made before absolute flows can be reliably measured.
authors.affiliation: Department of Pediatrics, Wayne State University School of Medicine, Detroit, Michigan, USA. tsingh@dmc.org
authors.forename: T P
authors.initials: TP
authors.lastname: Singh
authors.forename: K
authors.initials: K
authors.lastname: Greer
authors.forename: O
authors.initials: O
authors.lastname: Muzik
authors.forename: R L
authors.initials: RL
authors.lastname: Hammond
authors.forename: L W
authors.initials: LW
authors.lastname: Stephenson
authors.forename: M F
authors.initials: MF
authors.lastname: Di Carli
mesh_terms: D000818:Animals; D004285:Dogs; D005240:Feasibility Studies; D008863:Microspheres; D009119:Muscle Contraction; D009590:Nitrogen Radioisotopes; D011868:Radioisotopes; D019739:Skeletal Muscle Ventricle / Q000098:blood supply* / Q000502:physiology; D014055:Tomography, Emission-Computed
chemical_list: D009590:Nitrogen Radioisotopes; D011868:Radioisotopes