title: Production of wheat doubled haploids by pollination with Job's tears (Coix lachryma-jobi. L.).
abstract: Wheat (Triticum aestivum L.) haploids were produced by crossing with Job's tears (Coix lachryma-jobi L.) as the pollen parent. Pollination was followed by 2,4-D treatment, detached tiller culture, and embryo culture, as described for maize pollination. The frequency of embryo formation was similar to that obtained by crossing wheat with maize pollen. Job's tears is a perennial plant which forms several stalks and its pollen can be collected throughout the year when the plant is maintained in a controlled environment. Our results indicate that Job's tears can be used as the pollen parent for wheat crosses for haploid production without requiring synchronization of flowering dates.
authors.affiliation: Graduate School of Integrated Science and Kihara Institute for Biological Research, Yokohama City University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Mochida
authors.forename: H
authors.initials: H
authors.lastname: Tsujimoto
mesh_terms: D003433:Crosses, Genetic; D006238:Haploidy; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D010946:Plants, Medicinal / Q000235:genetics*; D014908:Triticum / Q000166:cytology / Q000235:genetics* / Q000254:growth & development