title: Expression of CD2 and activation markers on blood T-helper cell subsets in patients with transfusional iron overload.
abstract: The aim of the present study was to investigate the relationship between different measures of iron status, and the expression of CD2, and the activation markers CD25, CD71, CD45RO, HLADR CD38 within the Th-cell subset in patients with progressive transfusional iron overload. We estimated the expression of the activation surface markers on the Th cells of peripheral blood by flow cytometry from 22 multiply transfused patients. The number of CD2 binding sites (BS) on Th cells was significantly higher in the patients (82 917 +/- 30 801) than in age-matched normal controls (41 145 +/- 6989, P < 0.0001). When investigating whether this difference could be due to the iron overload we found the number of CD2 BS closely related to the iron saturation of serum transferrin (TfS) (R2 = 0.78, P < 0.001). The relationship to the serum ferritin concentration and to the number of blood units given was weaker, but also significant (R2 = 0.22, P < 0.027, respectively, R2 = 0.21, P < 0.032). Also the fraction of mature memory Th cells which express CD45RO at a high level was directly related to the TfS (R2 = 0.57, P < 0.0001), while the expression of CD38 within the Th cell fraction was inversely related to the TfS (R2 = - 0.43, P = 0.009). The expression of HLA-DR (but not of CD25 and CD71) was also directly related to the TfS (R2 = 0.29, P = 0.01). Our results show a clear, statistical relationship between the iron status and the expression of surface markers within Th cells in multiply transfused patients.
authors.affiliation: Department of Haematology, Aarhus Amtssygehus, Aarhus University Hospital, Denmark.
authors.forename: P D
authors.initials: PD
authors.lastname: Jensen
authors.forename: L
authors.initials: L
authors.lastname: Heickendorff
authors.forename: I
authors.initials: I
authors.lastname: Carlson
authors.forename: F T
authors.initials: FT
authors.lastname: Jensen
authors.forename: T
authors.initials: T
authors.lastname: Christensen
authors.forename: J
authors.initials: J
authors.lastname: Ellegaard
mesh_terms: D036541:ADP-ribosyl Cyclase; D051997:ADP-ribosyl Cyclase 1; D015703:Antigens, CD / Q000097:blood*; D000943:Antigens, Differentiation / Q000097:blood; D000944:Antigens, Differentiation, B-Lymphocyte / Q000097:blood; D018801:CD2 Antigens / Q000097:blood*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D005805:Genes, MHC Class I; D005838:Genotype; D006680:HLA Antigens / Q000235:genetics; D006684:HLA-DR Antigens / Q000097:blood; D000071020:Hemochromatosis Protein; D015395:Histocompatibility Antigens Class I / Q000235:genetics; D006801:Humans; D007156:Immunologic Memory; D007501:Iron / Q000097:blood*; D019190:Iron Overload / Q000276:immunology*; D017493:Leukocyte Common Antigens / Q000097:blood; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D009244:NAD+ Nucleosidase / Q000097:blood; D011990:Receptors, Transferrin; D012016:Reference Values; D012044:Regression Analysis; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*; D065227:Transfusion Reaction
chemical_list: D015703:Antigens, CD; D000943:Antigens, Differentiation; D000944:Antigens, Differentiation, B-Lymphocyte; D018801:CD2 Antigens; C068126:CD71 antigen; C103673:HFE protein, human; D006680:HLA Antigens; D006684:HLA-DR Antigens; D000071020:Hemochromatosis Protein; D015395:Histocompatibility Antigens Class I; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D011990:Receptors, Transferrin; D007501:Iron; D017493:Leukocyte Common Antigens; D036541:ADP-ribosyl Cyclase; C498154:CD38 protein, human; D009244:NAD+ Nucleosidase; D051997:ADP-ribosyl Cyclase 1