title: Characterization of whole blood aggregation with a new type of aggregometer by a screen filtration pressure method.
abstract: A new type of platelet aggregometer of whole blood, based on the screen filtration pressure method, has been developed and characterized. It measures resistance of flow of whole blood samples through a screen of microsieve with 30-microm(2) openings and provides pressure rate as an index of platelet aggregation. On optical microscopic observation, platelet aggregates, but not fibrin fibers, were found to be trapped on microsieves, and the pressure rate and protein amounts on microsieves are correlated. The aggregometer showed good reproducibility for investigations performed on different days. The time course of pressure rates indicated a bell curve change, where the pressure rate was very low immediately after blood collection and gradually increased up to 60 min thereafter. Use of the aggregometer was able to confirm that orally administered aspirin inhibits ADP- and collagen-induced whole blood aggregation as well as platelet aggregation. The results suggest that this platelet aggregometer might be useful in research and clinical diagnosis of thrombotic diseases.
authors.affiliation: 1st Institute of New Drug Research, Otsuka Pharmaceutical Co., Ltd., 463-10 Kagasuno, Kawauchi-cho, 771-0192, Tokushima, Japan. y_ozeki@research.otsuka.co.jp
authors.forename: Y
authors.initials: Y
authors.lastname: Ozeki
authors.forename: T
authors.initials: T
authors.lastname: Sudo
authors.forename: K
authors.initials: K
authors.lastname: Toga
authors.forename: Y
authors.initials: Y
authors.lastname: Nagamura
authors.forename: H
authors.initials: H
authors.lastname: Ito
authors.forename: T
authors.initials: T
authors.lastname: Ogawa
authors.forename: Y
authors.initials: Y
authors.lastname: Kimura
mesh_terms: D000244:Adenosine Diphosphate / Q000494:pharmacology; D001241:Aspirin / Q000008:administration & dosage / Q000494:pharmacology; D003094:Collagen / Q000494:pharmacology; D004867:Equipment Design; D005374:Filtration / Q000379:methods; D006801:Humans; D010974:Platelet Aggregation / Q000187:drug effects; D010979:Platelet Function Tests / Q000295:instrumentation*; D011312:Pressure; D015203:Reproducibility of Results
chemical_list: D000244:Adenosine Diphosphate; D003094:Collagen; D001241:Aspirin