title: Testicular sperm aspiration (TESA) and its application in oocyte donation.
abstract: This study was conducted to assess the outcomes of TESA in women undergoing oocyte donation. Descriptive reports from a university IVF practice were reviewed to evaluate the outcomes of women (n = 10) who underwent 12 cycles of ovum donation and required testicular sperm aspiration due to obstructive azoospermia (n = 6) and nonobstructive azoospermia (n = 4). Percutaneous needle aspiration of the testes was performed prior to oocyte retrieval to recover sperm, and intracytoplasmic sperm injection was subsequently performed on the donated oocytes. Fertilization was documented 16-18 h later and transcervical embryo transfer performed 72 h postretrieval. Successful retrieval of testicular spermatozoa utilizing the percutaneous technique was achieved in all 12 cycles. All cycles resulted in an ET with the achievement of 7 (58%) pregnancies, of which 5 (42%) are ongoing or delivered. The individual embryo implantation rate was 18.4% (9/49). Two cycles (16.6%) produced supernumerary embryos for cryopreservation. TESA represents a simple procurement technique associated with minimal morbidity. It may be offered even in cases of nondestructive azoospermia.
authors.affiliation: Department of Obstetrics/Gynecology, Columbia University, College of Physicians/Surgeons, New York, New York, USA. Steven.Lindheim@womenshealthusa.com
authors.forename: S R
authors.initials: SR
authors.lastname: Lindheim
authors.forename: K
authors.initials: K
authors.lastname: Crumm
authors.forename: H
authors.initials: H
authors.lastname: Fisch
authors.forename: M V
authors.initials: MV
authors.lastname: Sauer
mesh_terms: D000328:Adult; D002469:Cell Separation; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D007248:Infertility, Male; D008297:Male; D008875:Middle Aged; D018587:Oocyte Donation; D010043:Outcome and Process Assessment, Health Care; D011247:Pregnancy; D013094:Spermatozoa; D013396:Suction; D013737:Testis / Q000166:cytology*; D014019:Tissue Donors