Relationships between the shedding of Coxiella burnetii, clinical signs and serological responses of 34 sheep.
Two abortions associated with Coxiella burnetii occurred in a group of 34 pregnant ewes. The seroprevalence of C. burnetii infection was studied by using an ELISA and the immunofluorescence (IF) assay was applied to the contents of vaginal swabs. In addition, a PCR assay, with primers based on a transposon-like repetitive region of the C. burnetii genome (trans-PcR), was used for the highly sensitive and specific detection of C. burnetii in vaginal swabs, milk and faeces. Of the 34 animals tested at parturition, eight (24 per cent) were positive by ELISA, 11 (32 per cent) were positive by IF, and 15 (44 per cent) were positive when the vaginal swab extract was subjected to the trans-PCR assay. C. burnetii was therefore detected by PCR in the vaginal swabs of seven seronegative ewes. However, five weeks after lambing, 16 (47 per cent) of the animals tested were ELISA positive but only two animals (6 per cent) were positive by PCR. Among the ELISA- and PCR-positive animals, eight (25 per cent) shed coxiella in their milk and six (18 per cent) did so in their faeces.
Berri
M
M
Pathologie Infectieuse et Immunologie, INRA Tours-Nouzilly, France.
Souriau
A
A
Crosby
M
M
Crochet
D
D
Lechopier
P
P
Rodolakis
A
A
D000034:Abortion, Veterinary / Q000209:etiology*; D000818:Animals; D016997:Coxiella burnetii / Q000302:isolation & purification*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D019085:Fluorescent Antibody Technique, Direct; D005602:France / Q000453:epidemiology; D008892:Milk / Q000382:microbiology; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D011778:Q Fever / Q000150:complications / Q000453:epidemiology / Q000662:veterinary*; D016036:Seroepidemiologic Studies; D012756:Sheep; D012757:Sheep Diseases / Q000382:microbiology*; D014621:Vagina / Q000382:microbiology