Co-operativity between mouse connexin30 gap junction channels.
HeLa cells stably transfected with mouse cDNA coding for connexin30 (Cx30) were used to study the electrical properties of gap junction channels. The experiments involved the measurement of intercellular currents (Ij) from cell pairs using dual whole-cell recording with the patch-clamp method. The aim was to compare Ij from cell pairs whose gap junctions consisted of a single channel and cell pairs whose gap junctions consisted of many channels. We found that both the ensemble average currents gained from single-channel records and the currents obtained from multichannel records inactivated exponentially with time. However, the former inactivated significantly slower than the latter. At ajunctional voltage (Vj) of 50 mV, the time constants of inactivation (tau(i)) were 8.1 s and 1.6 s, respectively. Moreover, the ratio tau(i)(single-channel)/tau(i)(multichannel) turned out to be voltage sensitive, i.e. it decreased with increasing V(j) These observations suggest that the operation of Cx30 gap junction channels in the multichannel configuration involves co-operative interactions.
Valiunas
V
V
Department of Physiology, University of Bern, Switzerland.
Weingart
R
R
D000818:Animals; D000075302:Connexin 30; D017630:Connexins / Q000235:genetics / Q000378:metabolism*; D004558:Electric Stimulation; D017629:Gap Junctions / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D015640:Ion Channel Gating / Q000502:physiology; D007473:Ion Channels / Q000502:physiology; D008564:Membrane Potentials / Q000502:physiology; D051379:Mice; D018408:Patch-Clamp Techniques; D014162:Transfection
D000075302:Connexin 30; D017630:Connexins; C491020:GJB6 protein, human; C100629:Gjb6 protein, mouse; D007473:Ion Channels