title: Micellar electrokinetic capillary chromatography quantification of cytosine arabinoside and its metabolite, uracil arabinoside, in human serum.
abstract: Cytosine arabinoside (Ara-C) is widely used to induce remission in adult granulocytic leukemia. High doses can be infused in refractory leukemia or in relapse. After injection, Ara-C is quickly metabolized to uracil arabinoside (Ara-U), the main inactive metabolite. We here described a micellar electrokinetic capillary chromatography (MECC) method to simultaneously determine Ara-C/Ara-U in human serum using 6-O-methylguanine as an internal standard. The assay was linear from 6.25 to 200 microg/ml with a quantification limit between 3 and 6 microg/ml. The analytical precision was satisfactory between 2 and 4.3% (within-run) and 3.7 and 7.3% (between-runs). This assay was applied to the analysis of serum from acute granulocytic leukemia patient treated by high doses cytarabine (3 g/m2 body surface).
authors.affiliation: Laboratoire de Biochmie A, H pital Saint Louis, Paris, France.
authors.forename: P
authors.initials: P
authors.lastname: Houzé
authors.forename: F
authors.initials: F
authors.lastname: Deschamps
authors.forename: H
authors.initials: H
authors.lastname: Dombret
authors.forename: B
authors.initials: B
authors.lastname: Bousquet
authors.forename: B
authors.initials: B
authors.lastname: Gourmel
mesh_terms: D000964:Antimetabolites, Antineoplastic / Q000097:blood* / Q000627:therapeutic use; D001086:Arabinofuranosyluracil / Q000097:blood*; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D003561:Cytarabine / Q000097:blood* / Q000302:isolation & purification / Q000627:therapeutic use; D006801:Humans; D007951:Leukemia, Myeloid / Q000188:drug therapy; D009994:Osmolar Concentration; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013696:Temperature
chemical_list: D000964:Antimetabolites, Antineoplastic; D003561:Cytarabine; D001086:Arabinofuranosyluracil