gp49B1-alpha(v)beta3 interaction inhibits antigen-induced mast cell activation.
We have identified the integrin alpha(v)beta3 as a ligand for mouse gp49B1, thus identifying a new class of ligand for a member of the family of inhibitory immunoreceptors that bear C2-type immunoglobulin-like domains. The specific interaction was shown by both cell-protein and cell-cell binding assays. In addition, we found that the interaction of alpha(v)beta3 with gp49B1 on bone marrow-derived mouse mast cells inhibited antigen-induced immunoglobulin E-mediated cell activation. Because neither gp49B1 nor alpha(v)beta3 exhibit substantive allelic variation, their newly appreciated interaction may reflect an innate pathway for down-regulating the activity of mast cells.
Castells
M C
MC
Department of Medicine, Harvard Medical School and Division of Rheumatology, Immunology and Allergy, Brigham and Women's Hospital, Boston, MA 02115, USA. mariana_castells@hms.harvard.edu
Klickstein
L B
LB
Hassani
K
K
Cumplido
J A
JA
Lacouture
M E
ME
Austen
K F
KF
Katz
H R
HR
D000818:Animals; D000954:Antigens, Surface / Q000235:genetics / Q000378:metabolism*; D001667:Binding, Competitive; D001854:Bone Marrow Cells / Q000276:immunology; D007073:Immunoglobulin E / Q000276:immunology; D016023:Integrins / Q000235:genetics / Q000378:metabolism*; D008024:Ligands; D008407:Mast Cells / Q000276:immunology*; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D011485:Protein Binding; D011971:Receptors, Immunologic / Q000235:genetics / Q000378:metabolism*; D019030:Receptors, Vitronectin / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D019096:Vitronectin / Q000378:metabolism
D000954:Antigens, Surface; C489447:Gp49a protein, mouse; D016023:Integrins; D008024:Ligands; C489448:Lilrb4 protein, mouse; D008562:Membrane Glycoproteins; D011971:Receptors, Immunologic; D019030:Receptors, Vitronectin; D011994:Recombinant Proteins; D019096:Vitronectin; D007073:Immunoglobulin E