Hydrolysis of rice hull by crosslinked Aspergillus niger cellulase.
A. niger cellulase was crosslinked by glutaraldehyde to obtain a heat-stable enzyme preparation for rice hull cellulose hydrolysis. Under optimized crosslinking conditions of 0.12 M glutaraldehyde, pH 7.0, temperature 40 degrees C and at 45 min of crosslinking, a preparation having 15% more activity than free enzyme was obtained which also had considerable improvement in heat stability at 65 degrees C and 70 degrees C. Whereas the free enzyme lost 80% of its activity in 4 h at 65 degrees C, the crosslinked preparation lost only 30% activity. The crosslinked preparation hydrolyzed cellulosic biomass more effectively giving 2.2 mg/ml glucose and 52% corresponding saccharification in 4 h at 65 degrees C as compared to 14% saccharification by free enzyme under similar conditions.
Sharma
A
A
Chemistry Department, Indian Institute of Technology, New Delhi.
Khare
S K
SK
Gupta
M N
MN
D000821:Animal Feed; D000818:Animals; D001234:Aspergillus niger / Q000201:enzymology*; D018533:Biomass; D002480:Cellulase / Q000737:chemistry / Q000378:metabolism*; D002482:Cellulose / Q000378:metabolism*; D003432:Cross-Linking Reagents; D004795:Enzyme Stability; D005976:Glutaral / Q000494:pharmacology*; D006358:Hot Temperature; D006868:Hydrolysis; D007700:Kinetics; D012275:Oryza; D013816:Thermodynamics
D003432:Cross-Linking Reagents; D002482:Cellulose; D002480:Cellulase; D005976:Glutaral