Estimation of changes in tubulin induced with etoposide in human leukemia cells line K-562 by immunofluorescence microscope.
This study was undertaken to examine the influence of etoposide on the distribution of tubulin in cells of human leukemia cell line K-562 by using immunofluorescence method. The cells were cultured with 5 different doses of etoposide: 0.2, 2, 20, 200, 2000 microM/ml. Changes in tubulin were dependant on concentration of the drug compared to untreated control cells. The changes occurred in distribution of tubulin in cells K-562 after treating them with 200 and 2000 microM/ml etoposide. Cells treated with 200 microM/ml etoposide showed intense diffuse staining of the entire cells. At the surface of the cells there were also intensively stained structures extended outwards from the surface as lamellipodias. Cells cultured with 2000 microM/ml etoposide were much bigger from other cells exposed to lower doses of etoposide and control cells. Distribution of tubulin throughout these cells with diffuse intensive labeling of the cell periphery was seen. Very bright protrusions formed in lamellipodias at the surface of the cells were also observed.
Grzanka
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Department of Clinical Pathomorphology, University School of Medical Sciences, Bydgoszcz, Poland. grzanka@wsp.bydgoszcz.pl
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005047:Etoposide / Q000494:pharmacology*; D006801:Humans; D004915:Leukemia, Erythroblastic, Acute / Q000473:pathology*; D008856:Microscopy, Fluorescence; D011554:Pseudopodia / Q000187:drug effects; D014404:Tubulin / Q000378:metabolism*; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000972:Antineoplastic Agents, Phytogenic; D014404:Tubulin; D005047:Etoposide