[Effects of heat shock protein 70 overexpression on apoptosis of K562 cell caused by hyperthermia].
In order to observe the effects of heat shock protein 70(HSP70) on the apoptosis of K562 cell caused by hyperthermia, HSP70 was induced by pre-heating K562 cells at 40 degrees C and the levels of HSP70 were detected by RT-PCR. Cell apoptosis caused by hyperthermia at 43 degrees C was observed by Fluid cytometry (FCM) DNA content analyses. The results showed that HSP70 increased with the prolong of pre-heating time and reached the top level on pre-heating for 120 min and lasted for several hours. A lower apoptosis rate and less DNA ladder forming in the pre-heated cells than those not pre-heated. It is concluded that the higher expression of HSP70 induced by pre-heating treatment could inhibited the apoptosis caused by hyperthermia.
Wang
F
F
Faculty of Prevention Medicine, Fourth Military Medical University, Xi-an 710032, China.
Li
B
B
Yang
B
B
Yu
F
F
D017209:Apoptosis; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics; D006358:Hot Temperature; D006801:Humans; D020014:K562 Cells / Q000166:cytology / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics
D018840:HSP70 Heat-Shock Proteins; D012333:RNA, Messenger