Endocarditis caused by culture-negative organisms visible by Brown and Brenn staining: utility of PCR and DNA sequencing for diagnosis.
Two cases of culture-negative endocarditis with cocci seen in valve vegetations are presented. The organisms were identified by molecular analysis using broad-range PCR primers complementary to the 16S rRNA gene, sequencing, and database search using BLAST software. The results and utility of this method are discussed.
Wilck
M B
MB
Department of Laboratory Medicine, Yale University School of Medicine, New Haven, CT 06504, USA.
Wu
Y
Y
Howe
J G
JG
Crouch
J Y
JY
Edberg
S C
SC
D000328:Adult; D003470:Culture Media; D004697:Endocarditis, Bacterial / Q000175:diagnosis* / Q000382:microbiology*; D005260:Female; D020459:Genes, rRNA; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012984:Software; D013194:Staining and Labeling; D013203:Staphylococcal Infections / Q000175:diagnosis / Q000382:microbiology; D013210:Staphylococcus / Q000145:classification* / Q000235:genetics; D013290:Streptococcal Infections / Q000175:diagnosis / Q000382:microbiology; D013291:Streptococcus / Q000145:classification* / Q000235:genetics
D003470:Culture Media; D012336:RNA, Ribosomal, 16S