title: Noninstrumented enzyme-linked immunosorbant assay for detection of early pregnancy in macaques.
abstract: A practical, noninstrumented enzyme-linked immunosorbant assay (NELISA) for the measurement of urinary monkey chorionic gonadotropin (mCG) has been developed for the detection of early pregnancy in macaque monkeys for use in both the laboratory and the field. Five rhesus monkeys (Macaca mulatta) and six crab-eating monkeys (Macaca fascicularis) were tested for the presence of mCG in urine on gestational days (GDs) 12 to 35. The mCG NELISA detected pregnancy as early as GD 14, with an average earliest detection at GD 16.5 +/- 1.4 (n = 11). Out of 90 tests, 27 false-negative and zero false-positive tests were obtained, for an accuracy of 70.0%. Without the aid of a spectrophotometer, the presence of mCG in pregnant monkey samples was indicated by a dark green color change. Nonpregnant monkey urine samples, on the other hand, exhibited no color change. These findings suggest that the simple, economical, and reliable urinary mCG NELISA may be useful for diagnosing early pregnancy in these and related species. Because capture and restraint are unnecessary for collecting urine samples, the mCG NELISA has widespread potential for confined and free-ranging animals.
authors.affiliation: Department of Cellular and Molecular Biology, Primate Research Institute, Kyoto University, Inuyama, Aichi, Japan. shimizu@pri.kyoto-u.ac.jp
authors.forename: K
authors.initials: K
authors.lastname: Shimizu
authors.forename: P N
authors.initials: PN
authors.lastname: Lohstroh
authors.forename: L S
authors.initials: LS
authors.lastname: Laughlin
authors.forename: N A
authors.initials: NA
authors.lastname: Gee
authors.forename: H
authors.initials: H
authors.lastname: Todd
authors.forename: S E
authors.initials: SE
authors.lastname: Shideler
authors.forename: B L
authors.initials: BL
authors.lastname: Lasley
mesh_terms: D000818:Animals; D000830:Animals, Laboratory; D000835:Animals, Wild; D006063:Chorionic Gonadotropin / Q000276:immunology / Q000652:urine*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D005260:Female; D008253:Macaca mulatta / Q000502:physiology*; D011247:Pregnancy; D011259:Pregnancy Tests, Immunologic / Q000379:methods / Q000662:veterinary*; D012680:Sensitivity and Specificity
chemical_list: D006063:Chorionic Gonadotropin