Functional role of the HIV-1 Rev exon 1 encoded region in complex formation and trans-dominant inhibition.
To study functional aspects of the exon 1 encoded region of the human immunodeficiency virus type 1 Rev protein, the viral Tev protein which exhibits low Rev activity but lacks the rev exon 1 encoded region was examined. Neither Rev-Tev heteromer complex formation nor inhibition of Rev by an export deficient Tev mutant was observed. Insertion of the rev exon 1 encoded region into the Tev mutant allowed it to oligomerize with Rev and act as a trans-dominant negative mutant. This showed that the exon 1 encoded region of Rev is essential for oligomerization and that oligomerization is a prerequisite for trans-dominant inhibition.
Gjerdrum
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Department of Molecular Biology, University of Bergen, HIB, P.O. Box 7800, N-5020, Bergen, Norway.
Stranda
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Szilvay
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AM
D000818:Animals; D016466:CHO Cells; D019556:COS Cells; D006224:Cricetinae; D005091:Exons / Q000235:genetics*; D005455:Fluorescent Antibody Technique; D015694:Gene Products, rev / Q000037:antagonists & inhibitors / Q000502:physiology*; D005799:Genes, Dominant; D015497:HIV-1 / Q000235:genetics*; D046911:Macromolecular Substances; D009154:Mutation; D011485:Protein Binding / Q000502:physiology; D013489:Suppression, Genetic / Q000502:physiology*; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism; D014162:Transfection; D014760:Viral Fusion Proteins / Q000235:genetics / Q000378:metabolism; D054321:rev Gene Products, Human Immunodeficiency Virus
D015694:Gene Products, rev; D046911:Macromolecular Substances; D015534:Trans-Activators; D014760:Viral Fusion Proteins; D054321:rev Gene Products, Human Immunodeficiency Virus; C064085:tev protein p28, Human immunodeficiency virus 1