title: Identification and analysis of the Georgia 98 serotype, a new serotype of infectious bronchitis virus.
abstract: Twenty-five field infectious bronchitis viruses (IBVs) similar to, but genetically distinct from, the DE072 serotype were isolated from several states in the United States from 1990 through 1999 and were examined molecularly and antigenically. A 421-bp sequence in the hypervariable region of the S1 gene was examined, and phylogenetic analysis on that region indicated that these viruses are closely related but fall into unique groups. Cross-virus neutralization testing and entire S1 sequence analysis on selected isolates further confirmed that fact, and we divided the viruses into the DE072 serotype and two other unique groups. In a vaccine protection trial, the commercially available DE072 vaccine showed less than 50% protection against viruses in one of the groups. The majority of the recent isolates belong to that group and share very low antigenic relatedness to the DE072 strain as well as other serotypes of IBV. Consequently, we designated this group as a new serotype, Georgia 98. We developed a restriction fragment length polymorphism analysis that can differentiate this new serotype from all other serotypes of IBV.
authors.affiliation: Department of Avian Medicine, College of Veterinary Medicine, The University of Georgia, Athens 30602, USA.
authors.forename: C W
authors.initials: CW
authors.lastname: Lee
authors.forename: D A
authors.initials: DA
authors.lastname: Hilt
authors.forename: M W
authors.initials: MW
authors.lastname: Jackwood
mesh_terms: D000818:Animals; D000940:Antigenic Variation / Q000235:genetics; D002642:Chick Embryo; D002645:Chickens; D004279:DNA, Viral / Q000737:chemistry; D001351:Infectious bronchitis virus / Q000145:classification* / Q000235:genetics; D009500:Neutralization Tests / Q000662:veterinary; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D012703:Serotyping; D014481:United States; D014765:Viral Vaccines
chemical_list: D004279:DNA, Viral; D014765:Viral Vaccines