Effect of trehalose in low concentration on the binding and transport of porphyrins in liposome-human serum albumin system.
The influence of trehalose on the interaction of liposomes with porphyrins and with human serum albumin (HSA) was studied. Small unilamellar liposomes were prepared from 1,2-dimyristoyl-sn-glycero-3-phosphatidylcholine (DMPC) and from DMPC/1,2-dimyristoyl-sn-glycero-3-phosphatidylglycerol (DMPG) 19:1 w/w% and incorporated with mesoporphyrin IX (MP) or magnesium mesoporphyrin (MgMP). The fluorescence intensity and anisotropy of porphyrins were measured within the temperature range of 15-33 degrees C, in the presence and in the absence of 3x10(-2) M trehalose, to study the location of the porphyrins inside the liposomes and their partition between the liposomes and HSA. Based on the presented data and our earlier results (I. Bárdos-Nagy, R. Galántai, A.D. Kaposi, J. Fidy, Int. J. Pharm. 175 (1998) 255-267) we conclude that trehalose - even at this relatively low concentration - interacts with the head groups of the liposomes and that the presence of DMPG enhances the effect. This effect seems to hinder the binding of HSA to the liposome surface and influences the location of MgMP within the liposomes. In the case of MP, the porphyrin partition between the liposomes and HSA was affected by trehalose, while for MgMP, trehalose changed the structural conditions of porphyrin binding to the liposomes. The amount of trehalose used did not have a general trend to modify the association constants of porphyrin derivatives either to liposomes or to HSA.
Bárdos-Nagy
I
I
Department of Biophysics and Radiation Biology, Semmelweis University, Faculty of Medicine, POB 263, H-1444, Budapest, Hungary. inagy@puskin.sote.hu
Galántai
R
R
Fidy
J
J
D001692:Biological Transport / Q000187:drug effects; D004337:Drug Carriers; D005454:Fluorescence Polarization; D006801:Humans; D006860:Hydrogen Bonding; D008081:Liposomes / Q000737:chemistry; D008652:Mesoporphyrins / Q000737:chemistry; D011166:Porphyrins / Q000737:chemistry / Q000378:metabolism*; D012709:Serum Albumin / Q000737:chemistry / Q000378:metabolism*; D013053:Spectrophotometry; D013696:Temperature; D014199:Trehalose / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*
D004337:Drug Carriers; D008081:Liposomes; D008652:Mesoporphyrins; D011166:Porphyrins; D012709:Serum Albumin; C039630:magnesium mesoporphyrin; D014199:Trehalose