Purification and characterization of the enantioselective nitrile hydratase from Rhodococcus equi A4.
The nitrile hydratase from Rhodococcus equi A4 consisted of two kinds of subunits which slightly differed in molecular weight (both approximately 25 kDa) and showed a significant similarity in the N-terminal amino acid sequences to those of the nitrile hydratase from Rhodococcus sp. N-774. The enzyme preferentially hydrated the S-isomers of racemic 2-(2-, 4-methoxyphenyl)propionitrile, 2-(4-chlorophenyl)propionitrile and 2-(6-methoxynaphthyl)propionitrile (naproxennitrile) with E-values of 5-15. The enzyme functioned in the presence of 5-98% (v/v) of different hydrocarbons, alcohols or diisopropyl ether. The addition of 5% (v/v) of n-hexane, n-heptane, isooctane, n-hexadecane, pristane and methanol increased the E-value for the enzymatic hydration of 2-(6-methoxynaphthyl)propionitrile.
Prepechalová
I
I
Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague.
Martínková
L
L
Stolz
A
A
Ovesná
M
M
Bezouska
K
K
Kopecký
J
J
Kren
V
V
D000595:Amino Acid Sequence; D006836:Hydro-Lyases / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D006838:Hydrocarbons / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007526:Isoelectric Point; D000432:Methanol / Q000494:pharmacology; D008970:Molecular Weight; D009570:Nitriles / Q000378:metabolism*; D021122:Protein Subunits; D016929:Rhodococcus equi / Q000201:enzymology*; D012997:Solvents / Q000494:pharmacology; D013237:Stereoisomerism; D013379:Substrate Specificity; D013696:Temperature
D006838:Hydrocarbons; D009570:Nitriles; D021122:Protein Subunits; D012997:Solvents; D006836:Hydro-Lyases; C048831:nitrile hydratase; D000432:Methanol