[Effect of AVP(4-8) administration on Ca2+/CaM-dependent protein kinase II autophosphorylation in rat brain].
The extent increase of Ca2+/CaM-dependent protein kinase II (CaMK II) autophosphorylation in various brain regions of rat reached a maximum value, one hour after s.c. administration of AVP(4-8). The increase in the cortex amounted to 192% of the control (P < 0.001), while in the hippocampus only 40% (P < 0.05). The autophosphorylation of CaMK II was dependent on both Ca2+ and CaM. Western blotting with anti-CaMK II alpha monoclonal antibody showed that the content of CaMK II alpha in cortex did not show detectable change in 1 h as compared to the control group. ZDC(C)PR, an antagonist of AVP(4-8), markedly blocked the effect of AVP(4-8), suggesting that AVP (4-8) stimulated CaMK II autophosphorylation is mediated through its receptor.
Qiao
L Y
LY
Shanghai Institute of Biochemistry, Chinese Academy of Sciences, Shanghai 200031.
Chen
X F
XF
Gu
B X
BX
Wang
T X
TX
Du
Y C
YC
D000818:Animals; D001127:Arginine Vasopressin / Q000031:analogs & derivatives* / Q000494:pharmacology*; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000378:metabolism*; D002540:Cerebral Cortex / Q000201:enzymology*; D006624:Hippocampus / Q000201:enzymology*; D008297:Male; D010446:Peptide Fragments / Q000494:pharmacology*; D010766:Phosphorylation; D051381:Rats
D010446:Peptide Fragments; C066224:argipressin (4-8) cysteinyl methyl ester; D001127:Arginine Vasopressin; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; D017871:Calcium-Calmodulin-Dependent Protein Kinases