Physico-chemical analysis of lipid A fractions of lipopolysaccharide from Erwinia carotovora in relation to bioactivity.
Highly purified bisphosphoryl, monophosphoryl and dephosphoryl lipids A from Erwinia carotovora with different acylation patterns were characterized physico-chemically. Applying matrix assisted laser desorption/ionization mass spectrometry, the purity of the lipid A fractions was determined, and from monolayer measurements the molecular space requirement was estimated. Fourier transform infrared spectroscopy allowed the elucidation of the gel to liquid crystalline phase transition of the acyl chains as well as the determination of the tilt angle of the diglucosamine backbone with respect to the acyl chain direction applying dichroitic measurements with attenuated total reflectance. With synchrotron radiation small-angle X-ray diffraction the supramolecular aggregate structure was determined, and with fluorescence resonance energy transfer spectroscopy the lipopolysaccharide binding protein induced intercalation of lipid A into a phospholipid matrix corresponding to that of the macrophage membrane was investigated. From the results, a clear dependence of the physico-chemical parameters on the particular lipid A structure can be followed. Furthermore, these parameters correlate well with the biological activities of the various lipids A as deduced from their ability to induce biological activity (Limulus assay and cytokine induction in mononuclear cells). These results contribute to a closer interpretation of the physico-chemical prerequisites for endotoxic activity as found for enterobacterial lipid A.
Fukuoka
S
S
Shikoku National Industrial Research Institute, Takamatsu, Japan. fukuoka@sniri.go.jp
Brandenburg
K
K
Müller
M
M
Lindner
B
B
Koch
M H
MH
Seydel
U
U
D000388:Air; D004885:Erwinia / Q000737:chemistry / Q000502:physiology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000378:metabolism; D008033:Limulus Test; D008050:Lipid A / Q000031:analogs & derivatives / Q000032:analysis* / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000737:chemistry*; D008968:Molecular Conformation; D015394:Molecular Structure; D010743:Phospholipids / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013696:Temperature; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis; D014867:Water; D014961:X-Ray Diffraction
D008050:Lipid A; D008070:Lipopolysaccharides; D010743:Phospholipids; D014409:Tumor Necrosis Factor-alpha; D014867:Water