title: [Comparison of seminal vitamin B12, folate, reactive oxygen species and various sperm parameters between fertile and infertile males].
abstract: Vitamin B12(VB12), folate and reactive oxygen species(ROS) in human semen from both fertile and infertile males, and their relationships with other parameters were observed. Semen samples from 44 infertile and 176 fentile control subjects were collected and measured based on the guidelines issued by WHO. The results showed that no significant differences on semen VB12 and folate were observed between fertile and infertile groups(P > 0.05). However, the levels of ROS in infertile group were significantly higher than those in fertile group(P < 0.01). The morphological defects and low motility of sperm in infertile group was significantly higher than those in fertile group(P < 0.01). A positive relation was observed between the levels of ROS production and the morphological defect of sperm(P < 0.01). There was a significant negative correlation between the levels of VB12, folate and ROS(P < 0.01) in semen. It is interesting to note that the values of ROS also show a significantly positive correlation with sperm motility (P < 0.01). A significant differences of ROS levels between fertile and infertile group with negative white blood cells in sperm was also observed. It is concluded that the increase of ROS on the morphological defect of sperm is one of the most important factors related to poor sperm quality and human infertility.
authors.affiliation: Department of Clinical Nutrition, School of Public Health, Sun Yat-sen University of Medical Sciences, Guangzhou 510089, China.
authors.forename: Q
authors.initials: Q
authors.lastname: Chen
authors.forename: V
authors.initials: V
authors.lastname: Ng
authors.forename: J
authors.initials: J
authors.lastname: Mei
authors.forename: S E
authors.initials: SE
authors.lastname: Chia
mesh_terms: D000328:Adult; D005492:Folic Acid / Q000032:analysis*; D006801:Humans; D007248:Infertility, Male / Q000378:metabolism / Q000503:physiopathology; D008297:Male; D017382:Reactive Oxygen Species / Q000032:analysis*; D012661:Semen / Q000737:chemistry*; D013076:Sperm Count; D013081:Sperm Motility; D013094:Spermatozoa / Q000166:cytology; D014805:Vitamin B 12 / Q000032:analysis*
chemical_list: D017382:Reactive Oxygen Species; D005492:Folic Acid; D014805:Vitamin B 12