title: Allium sativum potentiates suicide gene therapy for murine transitional cell carcinoma.
abstract: This study evaluated the synergistic effect of Allium sativum (AS) with suicide gene therapy for transitional cell carcinoma (TCC) of the bladder. Subcutaneous TCCs were established in syngeneic C3H/He mice with 1 x 10(5) MBT-2 cells. AS liquid extract was injected at the site of tumor transplantation on Day 1 for three weeks (Experiment I) and into the established tumors weekly for five weeks (Experiment II) in combination with or without gene therapy using a replication-defective adenoviral vector containing a herpes simplex virus thymidine kinase (HSV-TK) gene under the transcriptional control of Rous sarcoma virus (RSV) promoter (Ad-RSV-TK, 5 x 10(8) plaque-forming units) plus ganciclovir (20 mg/kg/day i.p.). AS demonstrated a statistically significant reduction in incidence of TCC (cumulative dose 25 mg of AS). Combination AS-suicide gene therapy significantly inhibited the tumor growth compared with the controls, which was evidenced by apoptosis on histomorphological and immunohistochemical studies. These results suggest that AS had a definite antitumor effect in inhibiting tumorigenesis and growth of TCC in a murine model. AS treatment combined with suicide gene therapy had significant additive antitumor effects on TCC and may provide a novel and effective treatment modality for TCC of the bladder.
authors.affiliation: Department of Urology, Korea University College of Medicine, Seoul, Korea.
authors.forename: D G
authors.initials: DG
authors.lastname: Moon
authors.forename: J
authors.initials: J
authors.lastname: Cheon
authors.forename: D H
authors.initials: DH
authors.lastname: Yoon
authors.forename: H S
authors.initials: HS
authors.lastname: Park
authors.forename: H K
authors.initials: HK
authors.lastname: Kim
authors.forename: J J
authors.initials: JJ
authors.lastname: Kim
authors.forename: S K
authors.initials: SK
authors.lastname: Koh
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D017209:Apoptosis; D002295:Carcinoma, Transitional Cell / Q000628:therapy*; D003131:Combined Modality Therapy; D004195:Disease Models, Animal; D005260:Female; D015774:Ganciclovir; D005737:Garlic; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors; D007150:Immunohistochemistry; D051379:Mice; D008809:Mice, Inbred C3H; D008517:Phytotherapy; D011897:Random Allocation; D013937:Thymidine Kinase / Q000235:genetics; D001743:Urinary Bladder / Q000473:pathology; D001749:Urinary Bladder Neoplasms / Q000628:therapy*
chemical_list: D013937:Thymidine Kinase; D015774:Ganciclovir