Quantifying DNA damage by gel electrophoresis, electronic imaging and number-average length analysis.
DNA damages that can be converted to single- or double strand breaks can be quantified by separating DNA by gel electrophoresis and obtaining a quantitative image of the resulting distribution of DNA in the gel. We review the theory of this method and discuss its implementation, including the charge-coupled device (CCD) camera systems we developed to acquire images of fluorophore labeled DNA.
Sutherland
J C
JC
Biology Department, Brookhaven National Laboratory, Upton, NY 11973, USA. jcs@bnl.gov
Monteleone
D C
DC
Trunk
J G
JG
Bennett
P V
PV
Sutherland
B M
BM
D004247:DNA / Q000032:analysis / Q000302:isolation & purification; D004249:DNA Damage; D004587:Electrophoresis, Agar Gel / Q000295:instrumentation; D019075:Electrophoresis, Capillary; D005456:Fluorescent Dyes; D007091:Image Processing, Computer-Assisted / Q000295:instrumentation; D007834:Lasers; D010781:Photography; D012680:Sensitivity and Specificity
D005456:Fluorescent Dyes; D004247:DNA