H(2)O(2) produced by viridans group streptococci may contribute to inhibition of methicillin-resistant Staphylococcus aureus colonization of oral cavities in newborns.
In an accompanying report, we showed that viridans group streptococci may prevent methicillin-resistant Staphylococcus aureus (MRSA) colonization of the oral cavities of newborns. In the present study, we investigated the mechanism of prevention in vitro. Most viridans group streptococci had bacteriocin-like activity and killed MRSA, Burkholderia cepacia, Enterobacter aerogenes, and Pseudomonas aeruginosa; however, Escherichia coli, Enterobacter cloacae, and Candida albicans were resistant. The activity was induced only by H(2)O(2)-secreting strains and was inhibited by horseradish peroxidase or catalase in a dose-dependent manner. The mean concentration of H(2)O(2) produced by 18 strains of viridans group streptococci (1 x 10(8) cfu in 200 microL of culture medium+/-standard deviation was 1.24+/-0.60 mmol. Viridans group streptococci inhibited MRSA growth in saliva as well as in culture media. These results indicate that H(2)O(2) produced by viridans group streptococci may inhibit MRSA colonization of oral cavities in newborns.
Uehara
Y
Y
Division of Clinical Pathology, Nagano Children's Hospital, Toyoshina, Japan. ue3527@jeans.ocn.ne.jp
Kikuchi
K
K
Nakamura
T
T
Nakama
H
H
Agematsu
K
K
Kawakami
Y
Y
Maruchi
N
N
Totsuka
K
K
D000898:Antibiosis; D001430:Bacteriocins / Q000037:antagonists & inhibitors; D002374:Catalase / Q000378:metabolism; D003470:Culture Media; D006735:Horseradish Peroxidase / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism* / Q000494:pharmacology; D007231:Infant, Newborn; D016106:Methicillin Resistance; D009055:Mouth / Q000382:microbiology*; D013203:Staphylococcal Infections / Q000382:microbiology / Q000517:prevention & control; D013211:Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development*; D013291:Streptococcus / Q000254:growth & development / Q000378:metabolism*
D001430:Bacteriocins; D003470:Culture Media; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase; D002374:Catalase