Pax5 determines the identity of B cells from the beginning to the end of B-lymphopoiesis.
Despite being one of the most intensively studied cell types, the molecular basis of B cell specification is largely unknown. The Pax5 gene encoding the transcription factor BSAP is required for progression of B-lymphopoiesis beyond the pro-B cell stage. Pax5-deficient pro-B cells are, however, not yet committed to the B-lymphoid lineage, but instead have a broad lymphomyeloid developmental potential. Pax5 appears to mediate B-lineage commitment by repressing the transcription of non-B-lymphoid genes and by simultaneously activating the expression of B-lineage-specific genes. Pax5 thus functions both as a transcriptional repressor and activator, depending on its interactions with corepressors of the Groucho protein family or with positive regulators such as the TATA-binding protein. Once committed to the B-lineage, B cells require Pax5 function to maintain their B-lymphoid identity throughout B cell development.
Nutt
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Research Institute of Molecular Pathology, Dr. Bohr-Gasse 7, A-1030 Vienna, Austria.
Eberhard
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Horcher
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Rolink
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Busslinger
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D000818:Animals; D018941:Antigens, CD19 / Q000096:biosynthesis / Q000235:genetics; D016175:B-Lymphocyte Subsets / Q000166:cytology*; D002454:Cell Differentiation; D019070:Cell Lineage; D002478:Cells, Cultured; D018920:Coculture Techniques; D004268:DNA-Binding Proteins / Q000502:physiology; D018507:Gene Expression Regulation, Developmental; D015322:Gene Rearrangement, B-Lymphocyte; D005803:Genes, Immunoglobulin; D016259:Genes, myc; D006410:Hematopoiesis / Q000235:genetics*; D006801:Humans; D015851:Interleukin-7 / Q000502:physiology; D051379:Mice; D018345:Mice, Knockout; D008954:Models, Biological; D051757:PAX5 Transcription Factor; D011506:Proteins / Q000235:genetics / Q000502:physiology*; D012097:Repressor Proteins / Q000502:physiology; D017154:Stromal Cells / Q000166:cytology; D035362:Transcription Factor TFIID; D014157:Transcription Factors / Q000502:physiology; D019473:Transcription Factors, TFII / Q000378:metabolism; D014158:Transcription, Genetic
D018941:Antigens, CD19; D004268:DNA-Binding Proteins; D015851:Interleukin-7; D051757:PAX5 Transcription Factor; C497281:PAX5 protein, human; C497282:Pax5 protein, mouse; D011506:Proteins; D012097:Repressor Proteins; D035362:Transcription Factor TFIID; D014157:Transcription Factors; D019473:Transcription Factors, TFII