title: A marriage of bacteriology with cell biology results in twin arginines.
abstract: The Tat apparatus is a remarkable molecular machine required for the transmembrane translocation of folded proteins. Recent studies have identified jellyfish green fluorescent protein as an excellent reporter protein for the bacterial Tat pathway. Although the genetics of the Tat system are reasonably well-defined in Escherichia coli, the utilization of heterologous proteins as transport substrates promises to facilitate further mechanistic and structural characterization of this system.
authors.affiliation: Centre for Metalloprotein Spectroscopy and Biology, School of Biological Sciences, University of East Anglia, NR4 7TJ., Norwich, UK. f.sargent@uea.ac.uk
authors.forename: F
authors.initials: F
authors.lastname: Sargent
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism; D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001692:Biological Transport; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D020134:Catalytic Domain; D004926:Escherichia coli; D029968:Escherichia coli Proteins; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000235:genetics; D026901:Membrane Transport Proteins; D008969:Molecular Sequence Data; D009004:Monosaccharide Transport Proteins; D010089:Oxidoreductases, N-Demethylating / Q000235:genetics / Q000378:metabolism; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000235:genetics / Q000378:metabolism; D011485:Protein Binding; D000069816:SEC Translocation Channels; D000081416:SecA Proteins; D016415:Sequence Alignment
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D002352:Carrier Proteins; D029968:Escherichia coli Proteins; D008164:Luminescent Proteins; D026901:Membrane Transport Proteins; D009004:Monosaccharide Transport Proteins; D000069816:SEC Translocation Channels; C408585:twin-arginine translocase complex, E coli; D049452:Green Fluorescent Proteins; D010089:Oxidoreductases, N-Demethylating; C023186:trimethylamine dehydrogenase; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; C022358:mannitol PTS permease, E coli; D000251:Adenosine Triphosphatases; D000081416:SecA Proteins