Quantitative monitoring of circulating Epstein-Barr virus DNA for predicting the development of posttransplantation lymphoproliferative disease.
Epstein-Barr virus (EBV)-DNA was quantitatively measured to assess posttransplantation virus reactivation by real-time polymerase chain reaction (PCR). In the first retrospective analysis of a 7-year-old boy with lymphoproliferative disease (LPD) after an unrelated cord blood transplantation, serum EBV-DNA progressively increased to 4 x 10(5) copies/mL. EBV load was then prospectively monitored in peripheral blood from posttransplantation patients. The second case was an 8 year-old boy with aplastic anemia who received a CD34+ cell transplantation. This patient died of LPD with the progression of pulmonary nodules. EBV-DNA increased to 4 x 10(4) copies/mL after the control of cytomegalovirus reactivation. On the other hand, EBV-DNA was undetectable (<200 copies/mL) in the series of all 58 samples from 10 patients who did not develop LPD after hematopoietic stem cell transplantation. Sequential monitoring of circulating EBV-DNA by quantitative PCR may be a useful indicator for predicting the development of posttransplantation LPD.
Ohga
S
S
Department of Pediatrics, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan. ohgas@pediatr.med.kyushu-u.ac.jp
Kubo
E
E
Nomura
A
A
Takada
H
H
Suga
N
N
Ishii
E
E
Suminoe
A
A
Inamitsu
T
T
Matsuzaki
A
A
Kasuga
N
N
Hara
T
T
D000741:Anemia, Aplastic / Q000628:therapy; D002648:Child; D003587:Cytomegalovirus / Q000302:isolation & purification; D003586:Cytomegalovirus Infections / Q000821:virology; D004279:DNA, Viral / Q000097:blood*; D020031:Epstein-Barr Virus Infections / Q000209:etiology / Q000635:transmission / Q000821:virology*; D005199:Fanconi Anemia / Q000628:therapy; D017809:Fatal Outcome; D005312:Fetal Blood / Q000166:cytology / Q000821:virology; D005500:Follow-Up Studies; D018380:Hematopoietic Stem Cell Transplantation / Q000009:adverse effects*; D004854:Herpesvirus 4, Human / Q000254:growth & development / Q000302:isolation & purification*; D006801:Humans; D016867:Immunocompromised Host; D008232:Lymphoproliferative Disorders / Q000209:etiology / Q000821:virology*; D008297:Male; D016133:Polymerase Chain Reaction; D012189:Retrospective Studies; D014019:Tissue Donors; D019562:Viral Load; D014766:Viremia / Q000821:virology*; D014775:Virus Activation
D004279:DNA, Viral