Distribution of cells bearing B-cell alloantigen(s) in North Indian rheumatic fever/rheumatic heart disease patients.
Numerous investigators have developed monoclonal antibodies against B-cell alloantigen(s) of rheumatic fever. However, the developed monoclonals do not have the same significance in all the populations. We have developed a battery of monoclonals against B-cell alloantigens of North Indian rheumatic fever patients. In the present study, we have used these monoclonals to examine the frequency of rheumatic antigens in 30 patients with recurrence of rheumatic activity (RRA), 30 of rheumatic heart disease (RHD) patients and 50 controls using alkaline phosphatase anti-alkaline phosphatase (APAAP) technique. These patients were examined at the time of registry and after three months follow up. RRA patients showed higher percentage of lymphocyte positive as compare to RHD and controls. Interestingly, on follow-up RRA patients showed significant decline in positive lymphocyte as compare to first visit whereas no such change was observed in RHD patients. There were 90-93% of RRA and RHD patients positive with these monoclonals. A significant age variation of rheumatic cells was also noticed in all groups of rheumatic patients. We conclude that monoclonals raised from the same ethnic population are highly specific and cost effective to use them to develop an easy field test system such as APAAP, to identify the individual at risk, to develop rheumatic fever. It is also suggested that the alloantigen marker may persist through out life and gets activated after recurrence of the disease.
Kumar
D
D
Department of Experimental Medicine and Biotechnology, Postgraduate Institute of Medical Education and Research Chandigarh, India.
Kaul
P
P
Grover
A
A
Ganguly
N K
NK
D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D000954:Antigens, Surface; D001402:B-Lymphocytes / Q000276:immunology*; D002648:Child; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D005500:Follow-Up Studies; D006801:Humans; D007194:India; D007519:Isoantigens / Q000032:analysis / Q000097:blood*; D012213:Rheumatic Fever / Q000276:immunology*; D012214:Rheumatic Heart Disease / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; C082034:B-cell alloantigen D8-17; D007519:Isoantigens