Isolation and expression of a gene (CGR1) regulated during the yeast-hyphal transition in Candida albicans.
We used RNA fingerprinting of arbitrarily primed PCR to isolate genes upregulated during the yeast-hyphal transition in Candida albicans. The sequence and expression of one of these genes (CGR1, Candida growth regulation) are presented. Our results suggest that CGR1 expression is associated with a growth cessation of yeast cells, a prerequisite for germination in this organism.
Cho
T
T
Department of Oral Microbiology, Fukuoka Dental College, 2-15-1 Tamura, Sawara-ku, Fukuoka 814-0193, Japan. chot1@college.fdcnet.ac.jp
Sudoh
M
M
Tanaka
T
T
Nakashima
Y
Y
Chibana
H
H
Kaminishi
H
H
D000595:Amino Acid Sequence; D015152:Blotting, Northern; D002176:Candida albicans / Q000235:genetics* / Q000254:growth & development; D005656:Fungal Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012331:RNA, Fungal / Q000302:isolation & purification; D016415:Sequence Alignment
C426046:CGR1 protein, Candida albicans; D005656:Fungal Proteins; D012331:RNA, Fungal