Growth factor treatment enhances vestibular hair cell renewal and results in improved vestibular function.
The vestibules of adult guinea pigs were lesioned with gentamicin and then treated with perilymphatic infusion of either of two growth factor mixtures (i.e., GF I or GF II). GF I contained transforming growth factor alpha (TGFalpha), insulin-like growth factor type one (IGF-1), and retinoic acid (RA), whereas GF II contained those three factors and brain-derived neurotrophic factor. Treatment with GF I significantly enhanced vestibular hair cell renewal in ototoxin-damaged utricles and the maturation of stereociliary bundle morphology. The addition of brain-derived neurotrophic factor to the GF II infusion mixture resulted in the return of type 1 vestibular hair cells in ototoxin-damaged cristae, and improved vestibular function. These results suggest that growth factor therapy may be an effective treatment for balance disorders that are the result of hair cell dysfunction and/or loss.
Kopke
R D
RD
Departments of Defense Spatial Orientation Center and Otolaryngology, Naval Medical Center, San Diego, CA 92134, USA.
Jackson
R L
RL
Li
G
G
Rasmussen
M D
MD
Hoffer
M E
ME
Frenz
D A
DA
Costello
M
M
Schultheiss
P
P
Van De Water
T R
TR
D000818:Animals; D005839:Gentamicins / Q000008:administration & dosage; D006133:Growth Substances / Q000494:pharmacology*; D006168:Guinea Pigs; D018069:Hair Cells, Vestibular / Q000187:drug effects* / Q000502:physiology / Q000648:ultrastructure; D008854:Microscopy, Electron
D005839:Gentamicins; D006133:Growth Substances