title: No effect of methacrylate-based bone cement CMW 1 on the plasmatic phase of coagulation, red blood cells and endothelial cells in vitro.
abstract: The compatibility of a methacrylate-based bone cement (CMW 1, DePuy International Ltd, England) used for the fixation of joint prostheses was evaluated on plasma, an erythrocyte suspension and cultured human endothelial cells. The extract of the cement was tested, following 1 hour and 7 days of curing. After the contact in vitro of the extract with plasma, activated partial thromboplastin time, antithrombin III, thrombin-antithrombin complexes and fibrin degradation products were assayed. Hemolytic activity was tested by adding the cement extracts to a suspension of erythrocytes. After 4 hours of incubation at 37 degrees C, the hemoglobin concentration was determined on the supernatants by the colorimetric method. The effect of the cement on tissue factor and thrombomodulin production was evaluated on human umbilical vein endothelial cell cultures. Tissue factor was determined in cell lysates by enzyme immunoassay, following 4 hours' incubation of cultures with the cement extract. Thrombomodulin was assayed in cell lysates by enzyme immuno assay, after 24 hours' incubation with the cement extract. The response to all trans-retinoic acid (ATRA) was tested. The cement caused no significant modifications of the coagulation tests, had no hemolytic activity, did not determine tissue factor production and did not modify thrombomodulin, compared to the negative control. The response to stimulation with ATRA was similar to that of the negative control. We conclude that the cement extract does not affect the plasmatic phase of coagulation, has no effect on erythrocytes, does not induce the expression of procoagulant activity by endothelial cells and does not impair their antithrombotic property, within the limits of the tests performed.
authors.affiliation: Dipartimento Putti, Istituti  Ortopedici Rizzoli, Bologna, Italy. elisabetta.cenni@ior.it
authors.forename: E
authors.initials: E
authors.lastname: Cenni
authors.forename: G
authors.initials: G
authors.lastname: Ciapetti
authors.forename: D
authors.initials: D
authors.lastname: Granchi
authors.forename: S
authors.initials: S
authors.lastname: Stea
authors.forename: L
authors.initials: L
authors.lastname: Savarino
authors.forename: A
authors.initials: A
authors.lastname: Corradini
authors.forename: A
authors.initials: A
authors.lastname: Di Leo
mesh_terms: D001777:Blood Coagulation / Q000187:drug effects*; D001780:Blood Coagulation Tests; D001843:Bone Cements / Q000494:pharmacology*; D004353:Drug Evaluation, Preclinical; D004727:Endothelium / Q000166:cytology / Q000187:drug effects*; D004912:Erythrocytes / Q000187:drug effects*; D006454:Hemoglobins / Q000032:analysis / Q000187:drug effects; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D007124:Immunoenzyme Techniques; D008422:Materials Testing; D010949:Plasma / Q000187:drug effects*; D019904:Polymethyl Methacrylate / Q000494:pharmacology*; D018180:Thrombomodulin / Q000032:analysis / Q000187:drug effects; D013925:Thromboplastin / Q000032:analysis / Q000187:drug effects; D013997:Time Factors
chemical_list: D001843:Bone Cements; D006454:Hemoglobins; D018180:Thrombomodulin; D019904:Polymethyl Methacrylate; D013925:Thromboplastin