A 28 kDa protein of the Bacillus thuringiensis serovar shandongiensis isolate 89-T-34-22 induces a human leukemic cell-specific cytotoxicity.
A 28 kDa protein that exhibits cytocidal activity specific for human leukemic T (MOLT-4) cells was purified from proteinase K-digested parasporal inclusion of a Bacillus thuringiensis serovar shandongiensis isolate. The N-terminal sequence of the protein was identical with that of the 32 kDa protein, regarded as a protoxin, of the inclusion proteins. The median effective concentration of this protein was 0.23 microg/ml against MOLT-4 cells and its specific activity was 7.9 times greater than that of the whole inclusion proteins. The 28 kDa protein induced necrosis-like cytotoxicity against MOLT-4 cells and the cytopathic effect with the passage of time was characterized by cell swelling, nuclear membrane isolation and chromatin condensation.
Lee
D
D
Biotechnology and Food Research Institute, Fukuoka Industrial Technology Center, Kurume, Japan.
Katayama
H
H
Akao
T
T
Maeda
M
M
Tanaka
R
R
Yamashita
S
S
Saitoh
H
H
Mizuki
E
E
Ohba
M
M
D000970:Antineoplastic Agents / Q000494:pharmacology; D001413:Bacillus thuringiensis / Q000737:chemistry / Q000302:isolation & purification*; D001426:Bacterial Proteins / Q000302:isolation & purification* / Q000494:pharmacology; D002467:Cell Nucleus / Q000187:drug effects; D005591:Chemical Fractionation; D002852:Chromatography, Ion Exchange; D003435:Crotalid Venoms; D004591:Electrophoresis, Polyacrylamide Gel; D019286:Endopeptidase K; D004731:Endotoxins / Q000302:isolation & purification; D006367:HeLa Cells / Q000187:drug effects; D006801:Humans; D015458:Leukemia, T-Cell; D008933:Mitochondrial Swelling / Q000187:drug effects; D009284:Naphthols; D009498:Neurotoxins; D009685:Nuclear Envelope / Q000187:drug effects; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000970:Antineoplastic Agents; D001426:Bacterial Proteins; D003435:Crotalid Venoms; D004731:Endotoxins; C018107:Mojave toxin; D009284:Naphthols; D009498:Neurotoxins; C412914:parasporin; C024573:AA-497; D019286:Endopeptidase K