Spectral karyotyping (SKY) of mouse meiotic chromosomes.
The spectral karyotyping procedure of in situ hybridization with chromosome-specific probes assigns a unique colour code to each of the 21 mouse mitotic chromosomes. We have adapted this procedure to meiotic prophase chromosomes, and the results show that each of the pachytene or metaphase I bivalents can be identified. This technique has the potential to recognize synaptic anomalies and chromosome-specific structural and behavioural characteristics. We confirm these potentials by the recognition of the heterologous synapsis of the X and Y chromosomes and by the variances of synaptonemal complex lengths for each of the colour-coded bivalents in eight prophase nuclei.
Heng
H H
HH
Center for Molecular Medicine and Genetics, Department of Pathology, Karmanos Cancer Institute, Wayne State University School of Medicine, Detroit, MI 48202, USA. hheng@cmb.biosci.wayne.edu
Liu
G
G
Lu
W
W
Bremer
S
S
Ye
C J
CJ
Hughes
M
M
Moens
P
P
D000818:Animals; D020223:Chromosome Painting / Q000379:methods*; D007621:Karyotyping / Q000379:methods*; D008297:Male; D008540:Meiosis / Q000235:genetics*; D051379:Mice; D011418:Prophase / Q000235:genetics; D013737:Testis / Q000166:cytology; D014960:X Chromosome / Q000235:genetics; D014998:Y Chromosome / Q000235:genetics