Engineering variegated floral patterns in tobacco plants using the Arabidopsis transposable element Tag1.
Variegated flower phenotypes were generated using the Arabidopsis transposon Tag1 and the maize R regulatory gene. Tag1 was inserted between the CaMV 35S promoter and the maize R gene and transformed into tobacco plants. In half of the transgenic plants, variegated flower patterns were observed. Each line had a different pattern, with varying intensities with three lines showing only tiny sectors indicative of late excision and one showing large sectors indicative of earlier excision.
Liu
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Division of Biology, University of California at San Diego, La Jolla, CA 92093-0116, USA.
Galli
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Crawford
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D017360:Arabidopsis / Q000235:genetics*; D018983:DNA Footprinting; D017931:DNA Primers; D018744:DNA, Plant / Q000235:genetics / Q000302:isolation & purification; D016254:Mutagenesis, Insertional; D018547:Plant Stems / Q000502:physiology; D030821:Plants, Genetically Modified / Q000502:physiology; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D014026:Nicotiana / Q000235:genetics*; D014158:Transcription, Genetic; D014170:Transformation, Genetic; D003313:Zea mays / Q000235:genetics
D017931:DNA Primers; D018744:DNA, Plant