[Preparation of novel macroporous silica-based amide-polymer-bonded packing and its application to the separation of proteins].
A novel macroporous silica-based amide-polymer-bonded packing for protein separations in HPLC is described. The macroporous silica support was bonded with diethoxymethyl vinyl silane and then copolymerized with methylacrylamide and divinylbenzene to produce a tailored stationary phase with high resolution and inertness. The repeatability of packing preparation is good. They were characterized through the application of a standard protein mixture of pepsin, glucose oxidase, bean trypsinogen inhibitor and ribonuclease. The time for elution of these proteins is less than 12 minutes. It is suggested that the macroporous silica-based amide-polymer-bonded packing can be used to quickly separate biopolymer. This packing is a better alternative for the biopolymer separation.
Wei
Y
Y
Lanzhou Institute of Chemical Physics, The Chinese Academy of Sciences, Lanzhou, 730000.
Fan
L
L
Jiang
S
S
Chen
L
L
D002851:Chromatography, High Pressure Liquid; D005949:Glucose Oxidase / Q000302:isolation & purification*; D010434:Pepsin A / Q000302:isolation & purification*; D011506:Proteins / Q000302:isolation & purification; D012260:Ribonucleases / Q000302:isolation & purification*; D012822:Silicon Dioxide
D011506:Proteins; D012822:Silicon Dioxide; D005949:Glucose Oxidase; D012260:Ribonucleases; D010434:Pepsin A