[The synergistic effect of tetrandrine and rotundine on the action potential of rabbit sinus node dominant pacemaker cell and calcium currents of ventricular myocytes from guinea-pig].
Standard microelectrode technique and the whole-cell clamp technique were used to investigate the effects of tetrandrine and rotundine on the action potential of rabbit sinus node dominant pacemaker cell and calcium currents of ventricular myocytes from guinea-pig. The results indicate that: tetrandrine 1-200 mumol.L-1 and rotundine 3-300 mumol.L-1 decreased the action potential amplitude(APA), the maximal upstroke velocity (Vmax), spontaneous depolarizing velocity of phase 4(SP4) and prolonged the sinus circle length(SCL) in a dose-dependent manner. Tetrandrine 0.1 mumol.L-1 in combination with rotundine 0.3 mumol.L-1 was shown to effectively reduce the ICa of ventricular myotytes from guinea-pig, the rate of inhibition is 19.6%. The results suggest that the two drugs have a good synergistic effect on inhibiting calcium current. These results might also put forward a valuable theoretic base for using the two drugs together in treating patients.
Yan
S
S
Department of Pharmacology, Tongji Medical University, Wuhan 430030.
Yao
W X
WX
Xia
G J
GJ
Tong
Q S
QS
Jiang
M X
MX
D000200:Action Potentials / Q000187:drug effects; D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D044182:Benzylisoquinolines; D001600:Berberine Alkaloids / Q000494:pharmacology*; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D015220:Calcium Channels / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D005260:Female; D006168:Guinea Pigs; D008297:Male; D009206:Myocardium / Q000166:cytology*; D011817:Rabbits; D012849:Sinoatrial Node / Q000166:cytology / Q000502:physiology*
D000470:Alkaloids; D044182:Benzylisoquinolines; D001600:Berberine Alkaloids; D002121:Calcium Channel Blockers; D015220:Calcium Channels; C009438:tetrandrine; C014215:tetrahydropalmatine