Plasma osmolality dipsogenic thresholds and c-fos expression in the near-term ovine fetus.
In ovine and human pregnancy, fetal swallowing contributes importantly to amniotic fluid homeostasis. Fetal dipsogenic responsiveness to short-term plasma hyperosmolality develops in late gestation, although fetal swallowing is not stimulated in response to long-term plasma osmolality increases (2 to 3%), which typically stimulate adult drinking behavior. To explore the near-term fetal plasma osmolality threshold for swallowing stimulation, we examined the effects of i.v. hypertonic saline-induced subacute increases in plasma hypertonicity on fetal swallowing behavior. Central sites of activation were examined by c-fos expression in putative dipsogenic nuclei. The results demonstrate that subacute 2 to 3% plasma osmolality increases do not stimulate near-term ovine fetal swallowing. However, fetal swallowing activity significantly increased (3 times) after plasma osmolality increased >6% above basal values. Consistent with a specific dipsogenic response, i.v. hypertonic saline induced c-fos expression in the anterior third ventricle region, a putative dipsogenic center, as well as in the fetal hindbrain. The stimulation of fetal swallowing under conditions of higher osmotic stimulation and the correlation with forebrain c-fos expression indicates that near-term fetal osmoregulation mechanisms are functional, although not completely mature. Reduced fetal dipsogenic responsiveness may result from altered osmoreceptor sensitivity, downstream neuronal or synaptic immaturity, or potentially inhibitory actions of stimulated hindbrain nuclei.
Xu
Z
Z
Perinatal Research Laboratory, Department of Obstetrics and Gynecology, Harbor UCLA-Medical Center, Torrance, California 90502, USA. z_xu@prl.humc.edu
Nijland
M J
MJ
Ross
M G
MG
D000818:Animals; D001769:Blood; D003679:Deglutition; D005260:Female; D005333:Fetus / Q000502:physiology*; D015870:Gene Expression; D016762:Genes, fos; D006706:Homeostasis; D009994:Osmolar Concentration; D011247:Pregnancy; D012756:Sheep / Q000097:blood / Q000196:embryology*