title: Complexing of basic pancreatic proteinase inhibitor with soybean phospholipid multilamellar vesicles.
abstract: The formation of complexes of basic pancreatic proteinase inhibitor (BPTI) with multilamellar vesicles (MLV) from six preparations of soybean phospholipids of various composition was studied. When BPTI, a non-membrane protein, interacts with MLV, the vesicles aggregate, forming a precipitate of protein-lipid complexes. The BPTI content in the protein-lipid complexes increases with decreasing pH of the medium and on addition of negatively charged components into the lipid mixture. The protein-induced aggregation of the phospholipid vesicles is suggested to be mainly determined by electrostatic forces. The antiproteinase activity of BPTI in the complexes was rather low but increased up to 70% of the initial activity on addition of an ionic detergent (sodium deoxycholate).
authors.affiliation: School of Chemistry, Lomonosov Moscow State University, Moscow, 119899, Russia.
authors.forename: O P
authors.initials: OP
authors.lastname: Tiourina
authors.forename: T V
authors.initials: TV
authors.lastname: Sharf
authors.forename: A A
authors.initials: AA
authors.lastname: Selishcheva
authors.forename: G M
authors.initials: GM
authors.lastname: Sorokoumova
authors.forename: V I
authors.initials: VI
authors.lastname: Shvets
authors.forename: N I
authors.initials: NI
authors.lastname: Larionova
mesh_terms: D007611:Aprotinin / Q000737:chemistry* / Q000378:metabolism; D003840:Deoxycholic Acid / Q000494:pharmacology; D003902:Detergents / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006863:Hydrogen-Ion Concentration; D050356:Lipid Metabolism; D008055:Lipids / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D013025:Glycine max / Q000737:chemistry*; D013053:Spectrophotometry; D014357:Trypsin / Q000494:pharmacology; D014867:Water / Q000378:metabolism
chemical_list: D003902:Detergents; D008055:Lipids; D010743:Phospholipids; D003840:Deoxycholic Acid; D014867:Water; D007611:Aprotinin; D014357:Trypsin