Direct profiling of multiple enzyme activities in human cell lysates by affinity chromatography/electrospray ionization mass spectrometry: application to clinical enzymology.
We describe a new method for enzyme analysis using affinity capture followed by electrospray ionization mass spectrometry (ACESIMS) for the quantitative determination of the initial velocities of four heparin-modifying enzymes. These enzymes, when defective in affected children, lead to the lysosomal storage disease known as Sanfilippo syndrome. The method relies on substrates and internal standards conjugated to the molecular handle biotin via a heavy isotope-encodable, mass-adjustable linker. Reaction velocities of the Sanfilippo enzymes in a crude lysate prepared from as little as 2500 human skin fibroblasts can be determined. In addition, the ACESIMS method is widely applicable to the simultaneous analysis of multiple enzymes in a complex biological sample by a single analytical technique and will thus serve as a useful tool in basic and clinical biomedical research.
Gerber
S A
SA
Department of Chemistry, University of Washington, Seattle, Washington 98195-1700, USA.
Scott
C R
CR
Turecek
F
F
Gelb
M H
MH
D002478:Cells, Cultured; D002846:Chromatography, Affinity; D004798:Enzymes / Q000032:analysis*; D005347:Fibroblasts / Q000201:enzymology; D006801:Humans; D009084:Mucopolysaccharidosis III / Q000201:enzymology; D010641:Phenotype; D012867:Skin / Q000166:cytology; D021241:Spectrometry, Mass, Electrospray Ionization
D004798:Enzymes