title: Polar production of interleukin-8 by mesothelial cells promotes the transmesothelial migration of neutrophils: role of intercellular adhesion molecule-1.
abstract: Migration of polymorphonuclear neutrophils (PMNL) from the vascular compartment into the pleural space occurs rapidly during the development of parapneumonic effusions. This study investigated the polarized secretion of interleukin (IL)-8 in activated pleural mesothelial cells (PMC) and the migration of PMNL across resting, activated PMC monolayers. Results show that PMC produce IL-8 in a polar manner. When PMC were stimulated with Staphylococcus aureus or IL-1beta at the basal or at the apical surface, significantly (P< .05) more IL-8 was released toward the apical surface. This polarized production of IL-8 was confirmed by in situ hybridization. PMNL migration was higher from the basilar to apical than from the apical to basilar surface of PMC. Neutralizing antibodies against IL-8 and intercellular adhesion molecule (ICAM)-1 significantly (P< .001) blocked PMNL migration across activated monolayers. Thus, during pleural inflammation, PMC regulate the influx of PMNL into the pleural space by polar production of IL-8 and expression of ICAM-1.
authors.affiliation: Division of Pulmonary and Critical Care Medicine, Department of Veterans Affairs Medical Center, and Indiana University School of Medicine, Indianapolis, IN, USA.
authors.forename: N
authors.initials: N
authors.lastname: Nasreen
authors.forename: K A
authors.initials: KA
authors.lastname: Mohammed
authors.forename: J
authors.initials: J
authors.lastname: Hardwick
authors.forename: R D
authors.initials: RD
authors.lastname: Van Horn
authors.forename: K L
authors.initials: KL
authors.lastname: Sanders
authors.forename: C M
authors.initials: CM
authors.lastname: Doerschuk
authors.forename: J W
authors.initials: JW
authors.lastname: Hott
authors.forename: V B
authors.initials: VB
authors.lastname: Antony
mesh_terms: D002465:Cell Movement; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000276:immunology / Q000382:microbiology; D004848:Epithelium / Q000276:immunology*; D005434:Flow Cytometry; D006801:Humans; D017403:In Situ Hybridization; D066298:In Vitro Techniques; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis / Q000502:physiology*; D016209:Interleukin-8 / Q000032:analysis / Q000494:pharmacology; D007963:Leukocytes, Mononuclear; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology*; D010994:Pleura / Q000166:cytology; D013211:Staphylococcus aureus
chemical_list: D016209:Interleukin-8; D018799:Intercellular Adhesion Molecule-1