Identification of a novel nuclear guanosine triphosphate-binding protein differentially expressed in renal disease.
A novel guanosine triphosphate-binding protein, chronic renal failure gene (CRFG), was discovered by differential display PCR to be regulated differentially in renal disease. Within the rat kidney, CRFG mRNA was localized to the outer medulla and was highly expressed in epithelial cells. The specific renal expression of CRFG mRNA in the outer medulla was reduced dramatically in several rat models of renal disease, including diabetic nephropathy, partial nephrectomy, ischemia, and anti-Thy1.1-induced nephritis. CRFG was localized selectively in the nucleus of human and rodent cells, as determined by immunocytochemistry and green fluorescence fusion protein. Cellular mRNA levels of CRFG were also increased after serum administration, when cells proliferate. These data suggest that CRFG may be involved in regulating guanosine triphosphate-dependent nuclear events that are associated with cell proliferation and that are important in normal renal function and essential for growth and development.
Laping
Nicholas J
NJ
Department of Renal Pharmacology, SmithKline Beecham Pharmaceuticals, King of Prussia, Pennsylvania.
Olson
Barbara A
BA
Department of Renal Pharmacology, SmithKline Beecham Pharmaceuticals, King of Prussia, Pennsylvania.
Zhu
Yuan
Y
Department of Molecular Genetics, SmithKline Beecham Pharmaceuticals, King of Prussia, Pennsylvania.
D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D003928:Diabetic Nephropathies / Q000235:genetics / Q000378:metabolism; D004195:Disease Models, Animal; D019204:GTP-Binding Proteins / Q000235:genetics*; D015870:Gene Expression; D006801:Humans; D007511:Ischemia / Q000235:genetics / Q000378:metabolism; D007668:Kidney / Q000098:blood supply; D007674:Kidney Diseases / Q000235:genetics* / Q000378:metabolism; D007679:Kidney Medulla / Q000378:metabolism; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009393:Nephritis / Q000235:genetics / Q000378:metabolism; D009687:Nuclear Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D011916:Rats, Inbred F344; D011917:Rats, Inbred Lew; D017207:Rats, Sprague-Dawley; D011924:Rats, Zucker; D017386:Sequence Homology, Amino Acid
D017931:DNA Primers; D009687:Nuclear Proteins; D012333:RNA, Messenger; D004247:DNA; D019204:GTP-Binding Proteins; C498988:GTPBP4 protein, human; C498987:Gtpbp4 protein, rat