New method for determination of ten pesticides in human blood.
An analytical method was developed for precise identification and quantitation of 10 pesticides in human blood. The pesticides studied, which have appeared frequently in actual cases, were endosulfan, lindane, parathion, ethyl-azinphos, diazinon, malathion, alachlor, tetradifon, fenthion and dicofol (o-p' and p-p' isomers). The current method replaces an earlier method which involved liquid-liquid extraction with a mixture of n-hexane-benzene (1 + 1). The extraction is performed by solid-phase extraction, with C18 cartridges and 2 internal standards, perthane and triphenylphosphate. Eluates were analyzed by gas chromatography (GC) with nitrogen-phosphorus and electrochemical detectors. Results were confirmed by GC-mass spectrometry in the electron impact mode. Blood blank samples spiked with 2 standard mixtures and an internal standard were used for quantitation. Mean recoveries ranged from 71.83 to 97.10%. Detection and quantitation limits are reported for each pesticide. Examples are provided to show the application of the present method to actual samples.
García-Repetto
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Instituto Nacional de Toxicología, Departamento de Sevilla, Spain.
Giménez
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MP
Repetto
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M
D002138:Calibration; D002849:Chromatography, Gas; D004563:Electrochemistry; D005554:Forensic Medicine; D006801:Humans; D013058:Mass Spectrometry; D010575:Pesticides / Q000097:blood* / Q000506:poisoning; D011041:Poisoning / Q000175:diagnosis; D012015:Reference Standards; D015203:Reproducibility of Results
D010575:Pesticides