title: Carbohydrate binding properties of banana (Musa acuminata) lectin II. Binding of laminaribiose oligosaccharides and beta-glucans containing beta1,6-glucosyl end groups.
abstract: This paper extends our knowledge of the rather bizarre carbohydrate binding poperties of the banana lectin (Musa acuminata). Although a glucose/mannose binding protein which recognizes alpha-linked gluco-and manno-pyranosyl groups of polysaccharide chain ends, the banana lectin was shown to bind to internal 3-O-alpha-D-glucopyranosyl units. Now we report that this lectin also binds to the reducing glucosyl groups of beta-1,3-linked glucosyl oligosaccharides (e.g. laminaribiose oligomers). Additionally, banana lectin also recognizes beta1,6-linked glucosyl end groups (gentiobiosyl groups) as occur in many fungal beta1,3/1,6-linked polysaccharides. This behavior clearly distinguishes the banana lectin from other mannose/glucose binding lectins, such as concanavalin A and the pea, lentil and Calystegia sepium lectins.
authors.affiliation: Department of Biological Chemistry, The University of Michigan Medical School, Ann Arbor, MI, USA. igoldste@umich.edu
authors.forename: I J
authors.initials: IJ
authors.lastname: Goldstein
authors.forename: H C
authors.initials: HC
authors.lastname: Winter
authors.forename: H
authors.initials: H
authors.lastname: Mo
authors.forename: A
authors.initials: A
authors.lastname: Misaki
authors.forename: E J
authors.initials: EJ
authors.lastname: Van Damme
authors.forename: W J
authors.initials: WJ
authors.lastname: Peumans
mesh_terms: D001665:Binding Sites; D050260:Carbohydrate Metabolism; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000737:chemistry; D004187:Disaccharides / Q000737:chemistry / Q000378:metabolism; D005638:Fruit / Q000378:metabolism*; D005936:Glucans / Q000737:chemistry / Q000378:metabolism; D037102:Lectins / Q000302:isolation & purification / Q000378:metabolism*; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry / Q000378:metabolism; D037121:Plant Lectins
chemical_list: D002241:Carbohydrates; D004187:Disaccharides; D005936:Glucans; D037102:Lectins; D009844:Oligosaccharides; D037121:Plant Lectins; C014637:laminaribiose