Pharmacological properties of the mouse neurotensin receptor 3. Maintenance of cell surface receptor during internalization of neurotensin.
We recently reported the molecular identification of a new type of receptor for the neuropeptide neurotensin (NT), the neurotensin receptor 3 (NTR3), identical to sortilin, which binds receptor-associated protein. Here, we demonstrate that the cloned mouse NTR3 is expressed on the plasma membrane of transfected COS-7 cells. The mouse NTR3 is detectable by photoaffinity labeling and immunoblotting at the cell surface as a 100 kDa N-glycosylated protein. Biochemical analysis and confocal microscopic imaging clearly indicate that NT is efficiently internalized after binding to NTR3, and that despite this internalization, the amount of receptor present on the cell surface is maintained.
Navarro
V
V
Institut de Pharmacologie Moléculaire et Cellulaire, CNRS, UMR 6097, 660 route des Lucioles, 06560 Valbonne, France.
Martin
S
S
Sarret
P
P
Nielsen
M S
MS
Petersen
C M
CM
Vincent
J
J
Mazella
J
J
D033942:Adaptor Proteins, Vesicular Transport; D000818:Animals; D001692:Biological Transport; D015153:Blotting, Western; D019556:COS Cells; D002462:Cell Membrane / Q000378:metabolism; D015870:Gene Expression; D015151:Immunoblotting; D007457:Iodine Radioisotopes; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D018613:Microscopy, Confocal; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D009496:Neurotensin / Q000378:metabolism* / Q000493:pharmacokinetics; D019920:Photoaffinity Labels; D011869:Radioligand Assay; D018028:Receptors, Neurotensin / Q000235:genetics / Q000378:metabolism; D013347:Subcellular Fractions / Q000378:metabolism; D013379:Substrate Specificity; D014162:Transfection; D000099183:Sortilin
D033942:Adaptor Proteins, Vesicular Transport; D007457:Iodine Radioisotopes; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; D009496:Neurotensin; D019920:Photoaffinity Labels; D018028:Receptors, Neurotensin; D000099183:Sortilin