[Effects of tripchlorolide (T4) of Tripterygium Wilfordii Hook on the production of immunoglobulins by peripheral blood mononuclear cells and by synovial cells of rheumatoid arthritis patients in vitro].
Tripchlorolide(T4) is an active ingredient recently isolated from Tripterygium Wilfordii Hook. The in vitro effects of T4 on the peripheral blood mononuclear cells(PBMC) of healthy persons(n = 10), the digested single synovium cells(DSSC) (n = 3) and PBMC(n = 6) from RA patients on production of immunoglobulins (Ig) were studied using Elisa method. The results showed that T4 at concentrations from 5 ng.ml-1 to 35 ng.ml-1 significantly reduced the production of Ig by PWM-stimulated PBMC of healthy persons in a dose dependent manner. At concentration of 25 ng.ml-1, T4 also reduced the Ig secretion of RA-PBMC and DSSC. It is hoped that T4 would be an encouraging new drug of herbal nature for the treatment of RA.
Zeng
X J
XJ
Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and PUMC, Beijing 100730.
Zhang
N Z
NZ
D000328:Adult; D001172:Arthritis, Rheumatoid / Q000276:immunology / Q000473:pathology*; D002478:Cells, Cultured; D004224:Diterpenes / Q000302:isolation & purification / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis*; D007075:Immunoglobulin M / Q000096:biosynthesis*; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008297:Male; D010616:Phenanthrenes; D013583:Synovial Membrane / Q000166:cytology / Q000378:metabolism
D004224:Diterpenes; D004365:Drugs, Chinese Herbal; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007166:Immunosuppressive Agents; D010616:Phenanthrenes; C068051:tripchlorolide