Evaluation of two sperm antigens, rSMP-B and YWK-II, as targets for immunocontraception.
To determine whether sperm membrane components, rSMP-B and YWK-II, are suitable candidates as immunocontraceptives in humans, antifertility activities of the antibodies to the peptide fragments, rSMP-229 and rSMP-230 of rSMP-B and YAL-198 of YWK-II, were examined. In a previous report, anti-rSMP-230 antibody was shown to immobilise human sperm and to block human fertilisation, and the antigen (rSMP-230) to interact with antisperm antibodies found in sera of infertile women. Antibody to the second synthetic peptide, rSMP-229, corresponding to a different segment of rSMP-B, mimicked the biological activities of the anti-rSMP-230 antibody. Anti-YAL-198 antibody significantly, although weakly, inhibited human fertilisation. In the murine model, the anti-rSMP-B antibodies blocked in vitro fertilisation of mouse eggs but had no influence on embryo growth. Anti-YAL-198 antibody, however, arrested the growth of zygotes. In conclusion, rSMP-B, a human sperm protein, is a promising candidate in the development of an immunocontraceptive for human application. A second sperm protein, YWK-II, is effective as an antifertility immunogen in experimental animals.
Takikawa
M
M
Department of Obstetrics and Gynecology, School of Medicine, The University of Tokushima, Japan.
Kamada
M
M
Maegawa
M
M
Yamano
S
S
Irahara
M
M
Aono
T
T
Futaki
S
S
Ohmoto
Y
Y
Koide
S S
SS
D016564:Amyloid beta-Protein Precursor; D000818:Animals; D000906:Antibodies / Q000276:immunology* / Q000494:pharmacology; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000494:pharmacology; D000941:Antigens / Q000276:immunology*; D000954:Antigens, Surface; D003269:Contraception, Immunologic / Q000379:methods*; D005260:Female; D005307:Fertilization in Vitro / Q000187:drug effects; D006801:Humans; D007106:Immune Sera / Q000276:immunology / Q000494:pharmacology; D007246:Infertility / Q000139:chemically induced / Q000276:immunology*; D008297:Male; D008565:Membrane Proteins / Q000276:immunology*; D051379:Mice; D023421:Models, Animal; D009419:Nerve Tissue Proteins; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000276:immunology; D013084:Sperm-Ovum Interactions / Q000187:drug effects; D013094:Spermatozoa / Q000276:immunology*
C489697:APLP1 protein, human; C084104:APLP2 protein, human; D016564:Amyloid beta-Protein Precursor; D000906:Antibodies; D000911:Antibodies, Monoclonal; D000941:Antigens; D000954:Antigens, Surface; C078201:Aplp1 protein, mouse; C492952:Aplp2 protein, mouse; D007106:Immune Sera; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D010446:Peptide Fragments; C049242:rSMP-B protein, human