[Effect of ligustrazine on CD34 antigen expression of bone marrow cells in immune-mediated aplastic anemia mice].
OBJECTIVE
To explore the effect of ligustrazine on CD34 antigen expression of bone marrow cells in immune-mediated aplastic anemia (AA) mice.


METHODS
The model of immune aplastic anemia mice was induced by means of 6.0 Gy60Co gamma-ray irradiation and lymphocyte infusion through tail vein. The mice were divided into 3 groups: the normal group, the AA control group and the ligustrazine group. Mice of the ligustrazine group were fed by 4 mg of ligustrazine injection twice a day by gastrogavage. On the 10th day, CD34 antigen expression intensity of bone marrow cell membrane was measured by flow cytometer analysis system.


RESULTS
CD34 antigen expression intensity of ligustrazine group was 77.6 +/- 6.5, with no statistic difference from that in the normal group (80.0 +/- 2.6), while that of the control group was much higher (68.6 +/- 4.5, P < 0.05).


CONCLUSIONS
Ligustrazine could promote proliferation of stem and progenitor cell of AA mice through influencing on bone marrow micro-environment so as to increase the CD34 antigen expression of bone marrow cells.
Shu
Y
Y
Tongji Hospital, Tongji Medical University, Wuhan 430030.
Sun
H
H
Dong
L
L
D000741:Anemia, Aplastic / Q000188:drug therapy / Q000209:etiology / Q000276:immunology*; D000818:Animals; D018952:Antigens, CD34 / Q000096:biosynthesis* / Q000378:metabolism; D001854:Bone Marrow Cells / Q000276:immunology*; D003037:Cobalt Radioisotopes; D005260:Female; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008811:Mice, Inbred DBA; D011719:Pyrazines / Q000494:pharmacology* / Q000627:therapeutic use; D014665:Vasodilator Agents / Q000494:pharmacology* / Q000627:therapeutic use; D014916:Whole-Body Irradiation
D018952:Antigens, CD34; D003037:Cobalt Radioisotopes; D011719:Pyrazines; D014665:Vasodilator Agents; C017953:tetramethylpyrazine