[Lipopolysaccharide upregulates expression of CD14 gene and CD14 proteins of hepatocytes in rats].
OBJECTIVE
To observe the expression of CD14 mRNA and its protein in rat hepatocytes during endotoxemia.


METHODS
The acute endotoxemia model of wistar rats was established by injection of a dose of LPS (5mg/kg, Escherichia coli O111:B4) via the tail vein, then the rats were sacrificed at 3, 6, 12, and 24 hour, respectively. Hepatocytes were isolated from normal and LPS-injected rats by in situ collagenase perfusion technique. Hepatocytes and hepatic tissue were collected to measure the expression of CD14 mRNA and CD14 protein by reverse transcriptase-polymerase chain reaction (RT-PCR) or Western blot analysis. The binding of fluorescein isothiocyanate (FITC)-CD14 polyclone antibody to isolated hepatocytes was also assessed by flow cytometric analysis (FCM).


RESULTS
In the rats with endotoxemia, the expression of CD14 mRNA and CD14 protein in hepatic tissue and isolated hepatocytes were stronger at 6, 12 and 24 hour than that in control rats (P<0.01). FCM showed that positive cell numbers of FITC-CD14 in the rats with endotoxemia increased when compared with normal control group (P<0.01).


CONCLUSIONS
LPS can markedly upregulate the expression of CD14 mRNA and CD14 proteins in hepatic tissue and isolated hepatocytes.
Li
S
S
Second College of Clinical Medicine & Second Affiliated Hospital of Chongqing University of Medical Sciences, Chongqing 400010, China.
Wu
C
C
Shi
Y
Y
Liu
C
C
D000818:Animals; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D022781:Hepatocytes / Q000187:drug effects* / Q000378:metabolism; D018950:Lipopolysaccharide Receptors / Q000096:biosynthesis / Q000235:genetics*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008297:Male; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D015854:Up-Regulation
D018950:Lipopolysaccharide Receptors; D008070:Lipopolysaccharides; D012333:RNA, Messenger