title: Nucleotide sequence of the coat protein gene of Lettuce big-vein virus.
abstract: A sequence of 1425 nt was established that included the complete coat protein (CP) gene of Lettuce big-vein virus (LBVV). The LBVV CP gene encodes a 397 amino acid protein with a predicted M(r) of 44486. Antisera raised against synthetic peptides corresponding to N-terminal or C-terminal parts of the LBVV CP reacted in Western blot analysis with a protein with an M(r) of about 48000. RNA extracted from purified particles of LBVV by using proteinase K, SDS and phenol migrated in gels as two single-stranded RNA species of approximately 7.3 kb (ss-1) and 6.6 kb (ss-2). After denaturation by heat and annealing at room temperature, the RNA migrated as four species, ss-1, ss-2 and two additional double-stranded RNAs (ds-1 and ds-2). The Northern blot hybridization analysis using riboprobes from a full-length clone of the LBVV CP gene indicated that ss-2 has a negative-sense nature and contains the LBVV CP gene. Moreover, ds-2 is a double-stranded form of ss-2. Database searches showed that the LBVV CP most resembled the nucleocapsid proteins of rhabdoviruses. These results indicate that it would be appropriate to classify LBVV as a negative-sense single-stranded RNA virus rather than as a double-stranded RNA virus.
authors.affiliation: Shikoku National Agricultural Experiment Station, Zentsuji, Kagawa 765-8508, Japan1.
authors.forename: Takahide
authors.initials: T
authors.lastname: Sasaya
authors.affiliation: Shikoku National Agricultural Experiment Station, Zentsuji, Kagawa 765-8508, Japan1.
authors.forename: Koichi
authors.initials: K
authors.lastname: Ishikawa
authors.affiliation: Shikoku National Agricultural Experiment Station, Zentsuji, Kagawa 765-8508, Japan1.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Koganezawa
mesh_terms: D000595:Amino Acid Sequence; D020029:Base Pairing; D001483:Base Sequence; D015153:Blotting, Western; D002213:Capsid / Q000737:chemistry / Q000235:genetics*; D003001:Cloning, Molecular; D016679:Genome, Viral; D018545:Lactuca / Q000821:virology*; D008969:Molecular Sequence Data; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization; D010942:Plant Viruses / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D012328:RNA Viruses / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D012330:RNA, Double-Stranded / Q000032:analysis / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D012367:RNA, Viral / Q000032:analysis / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D012330:RNA, Double-Stranded; D012367:RNA, Viral