title: Multi-organ, multi-lineage engraftment by a single bone marrow-derived stem cell.
abstract: Purification of rare hematopoietic stem cell(s) (HSC) to homogeneity is required to study their self-renewal, differentiation, phenotype, and homing. Long-term repopulation (LTR) of irradiated hosts and serial transplantation to secondary hosts represent the gold standard for demonstrating self-renewal and differentiation, the defining properties of HSC. We show that rare cells that home to bone marrow can LTR primary and secondary recipients. During the homing, CD34 and SCA-1 expression increases uniquely on cells that home to marrow. These adult bone marrow cells have tremendous differentiative capacity as they can also differentiate into epithelial cells of the liver, lung, GI tract, and skin. This finding may contribute to clinical treatment of genetic disease or tissue repair.
authors.affiliation: Department of Laboratory Medicine and, Yale University School of Medicine, New Haven, CT 06520, USA. diane.krause@yale.edu
authors.forename: D S
authors.initials: DS
authors.lastname: Krause
authors.forename: N D
authors.initials: ND
authors.lastname: Theise
authors.forename: M I
authors.initials: MI
authors.lastname: Collector
authors.forename: O
authors.initials: O
authors.lastname: Henegariu
authors.forename: S
authors.initials: S
authors.lastname: Hwang
authors.forename: R
authors.initials: R
authors.lastname: Gardner
authors.forename: S
authors.initials: S
authors.lastname: Neutzel
authors.forename: S J
authors.initials: SJ
authors.lastname: Sharkis
mesh_terms: D000818:Animals; D018952:Antigens, CD34 / Q000378:metabolism*; D000950:Antigens, Ly / Q000378:metabolism*; D001854:Bone Marrow Cells / Q000166:cytology*; D019070:Cell Lineage; D002465:Cell Movement; D004847:Epithelial Cells / Q000166:cytology / Q000502:physiology; D005260:Female; D005456:Fluorescent Dyes / Q000378:metabolism; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000502:physiology; D006801:Humans; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D007421:Intestine, Small / Q000166:cytology; D007633:Keratins / Q000378:metabolism; D008168:Lung / Q000166:cytology; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D018345:Mice, Knockout; D009930:Organic Chemicals; D011663:Pulmonary Surfactants / Q000235:genetics / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology* / Q000502:physiology; D014998:Y Chromosome / Q000235:genetics / Q000378:metabolism
chemical_list: D018952:Antigens, CD34; D000950:Antigens, Ly; D005456:Fluorescent Dyes; C074650:Ly6a protein, mouse; D008565:Membrane Proteins; D009930:Organic Chemicals; C070080:PKH 26; D011663:Pulmonary Surfactants; D007633:Keratins