Antibacterial activity of 4,5-dihydroxy-2-cyclopentan-1-one (DHCP) and cloning of a gene conferring DHCP resistance in Escherichia coli.
In the present study we report that 4,5-dihydroxy-2-cyclopentan-1-one (DHCP), which is derived from heat-treatment of uronic acid or its derivatives, has antibacterial activity against Escherichia coli. The compound causes complete growth inhibition at 350 microM concentration. We have cloned a gene from E. coli, which confers DHCP resistance when present in multicopy. The putative protein encoded by this gene (dep- DHCP efflux protein) is a transmembrane efflux protein with a high homology to other antibiotic-efflux proteins including those for chloramphenicol, bicyclomycin and tetracycline. However, the Dep protein does not confer cross-resistance to any of the antibiotics tested.
Phadtare
S
S
Department of Biochemistry, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA.
Yamanaka
K
K
Kato
I
I
Inouye
M
M
D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics*; D002352:Carrier Proteins / Q000737:chemistry; D003001:Cloning, Molecular; D003517:Cyclopentanes / Q000494:pharmacology*; D004352:Drug Resistance, Microbial / Q000235:genetics*; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics* / Q000254:growth & development; D029968:Escherichia coli Proteins; D015698:Genomic Library; D007700:Kinetics; D008565:Membrane Proteins / Q000737:chemistry; D026901:Membrane Transport Proteins; D008969:Molecular Sequence Data; D019151:Peptide Library; D011487:Protein Conformation; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D012984:Software
C436749:4,5-dihydroxy-2-cyclopentan-1-one; D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D002352:Carrier Proteins; D003517:Cyclopentanes; D029968:Escherichia coli Proteins; C118438:Mdfa protein, E coli; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D019151:Peptide Library; C436750:dep-DHCP efflux protein, E coli