Effect of partial and sham hepatectomy on the growth of a hepatocellular carcinoma.
We examined the effect of partial hepatectomy on the proliferation of hepatoma ES12a grafted into C3H/S mice compared to tumor growth in sham-hepatectomized controls. The animals were sacrificed every 4 hrs during three days from the 6th to the 78th h following each type of surgery. Unoperated tumor-bearing mice were likewise killed as controls, but only during one complete circadian period. All animals received 2 microg of colchicine per g of body weight intraperitoneally 4 hrs before decapitation. Measurement of mitotic indices in hematoxylineosin-stained tumor samples revealed a decrease in proliferation and a modification of the diurnal mitotic-activity profile in the hepatectomized and sham-operated animals from the first day after surgery. These differences persisted by the third postoperative day only in the hepatectomized animals. Thus, although surgical stress may initially affect tumor growth, the latter results must be the effect of the influence of the hepatic regeneration.
Barbeito
C G
CG
Cátedra de Histología y Embriología A, Facultad de Ciencias Médicas, Universidad Nacional de La Plata, Republica Argentina. barbeito@fcv.medvet.unlp.edu.ar
Garcia
M N
MN
Flamini
M A
MA
Andrini
L B
LB
Badrán
A F
AF
D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000473:pathology* / Q000601:surgery*; D002455:Cell Division; D002940:Circadian Rhythm; D003078:Colchicine; D004396:Coloring Agents; D006498:Hepatectomy; D008113:Liver Neoplasms / Q000473:pathology* / Q000601:surgery*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008938:Mitosis; D008940:Mitotic Index; D013997:Time Factors
D004396:Coloring Agents; D003078:Colchicine