In vitro release of interferon-gamma by trichophytin-stimulated whole blood cell cultures from ringworm-vaccinated and control calves experimentally inoculated with Trichophyton verrucosum.
Of a group of seven calves, four were vaccinated against bovine ringworm with the vaccine Ringvac bovis LTF-130 (Alpharma, Oslo, Norway), while three calves were left unvaccinated. All calves were inoculated epicutaneously with a virulent strain of Trichophyton verrucosum. Clinical signs were recorded. In response to stimulation with trichophytin, in vitro interferon-gamma (IFN-gamma) production in whole blood cell cultures was assessed in samples obtained pre- and post-vaccination and pre- and post-inoculation. A commercial enzyme immunoas                say kit was used to measure IFN-gamma levels (Bovigam, CSL, Victoria, Australia). Control calves developed typical ringworm lesions, whereas vaccinated calves did not. Following vaccination, release of IFN-gamma in whole blood cell cultures indicated the presence of circulating trichophytin-specific lymphocytes. After inoculation with T. verrucosum, IFN-gamma production was demonstrated in samples from both vaccinated and control calves. This study showed that vaccination with Ringvac bovis LTF-130 elicits a protective immune response suggesting involvement of the cellular branch of the immune system. Experimental infection of naïve nonvaccinated calves with T. verrucosum, also indicated stimulation of a cell-mediated immune response essential for resolution of lesions.
Lund
A
A
Department of Immunoprohylaxis, National Veterinary Institute, POB 8156 Dep., 0033 Oslo, Norway. arve.lund@noragric.nlh.no
Bratberg
A M
AM
Solbakk
I T
IT
D000818:Animals; D000831:Animals, Newborn; D002417:Cattle; D002418:Cattle Diseases / Q000517:prevention & control*; D005657:Fungal Vaccines; D007371:Interferon-gamma / Q000097:blood*; D014005:Tinea / Q000517:prevention & control / Q000662:veterinary*; D014249:Trichophyton / Q000276:immunology*
D005657:Fungal Vaccines; D007371:Interferon-gamma