Elevated plasma stromal cell-derived factor 1 protein level in the progression of HIV type 1 infection/AIDS.
Stromal cell-derived factor 1 (SDF-1) is a unique chemokine involved in multiple organogenesis as well as in the regulation of HIV infection. Here we determined the plasma SDF-1 concentrations of 193 HIV-1-infected individuals and 154 normal Japanese volunteers by developing a highly sensitive measurement system based on time-resolved fluoroimmunoassay (SDF-1 TR-FIA). This system is also valid for the mouse model to quantitate circulating SDF-1 concentration in vivo and thereby its correlation with CXCR4 expression level on CD4(+) T cells. Interestingly, plasma SDF-1 concentrations in HIV-1-infected individuals were three times higher than those in a normal control group and plasma SDF-1 protein levels showed an inverse correlation with CD4(+) T cell count and a positive correlation with plasma HIV-1 RNA load. Notably, individuals with later stage HIV-1 infection, who maintained fewer than 200 CD4(+) T cells per cubic milliliter and more than 10,000 copies of HIV-1 RNA per milliliter, showed the highest plasma SDF-1 level among individuals at any stage of HIV-1 infection. These results suggest that endogenous SDF-1 is upregulated by HIV-1 infection, particularly in late-stage HIV-1 infection/AIDS.
Ikegawa
M
M
Department of Medical Chemistry, Kyoto University Faculty of Medicine, Sakyo-ku, Kyoto 606-8501, Japan.
Yuan
J
J
Matsumoto
K
K
Herrmann
S
S
Iwamoto
A
A
Nakamura
T
T
Matsushita
S
S
Kimura
T
T
Honjo
T
T
Tashiro
K
K
D000293:Adolescent; D000328:Adult; D000818:Animals; D019380:Anti-HIV Agents / Q000097:blood*; D018791:CD4 Lymphocyte Count; D015496:CD4-Positive T-Lymphocytes; D016022:Case-Control Studies; D054377:Chemokine CXCL12; D019743:Chemokines, CXC / Q000097:blood*; D018450:Disease Progression; D015200:Fluoroimmunoassay / Q000379:methods; D015658:HIV Infections / Q000097:blood* / Q000821:virology; D015497:HIV-1; D006801:Humans; D051379:Mice; D012680:Sensitivity and Specificity; D017154:Stromal Cells / Q000378:metabolism; D015854:Up-Regulation; D019562:Viral Load
D019380:Anti-HIV Agents; C515255:CXCL12 protein, human; D054377:Chemokine CXCL12; D019743:Chemokines, CXC; C515256:Cxcl12 protein, mouse