Commentary: possible mechanisms for strand-breaks in DNA induced by stirring.
When aqueous solutions of DNA are stirred at room temperature, strand breaks occur extensively. Using various spin-traps, coupled with epr spectroscopy, we have shown that this does not proceed via homolysis. It is suggested that breaks occur by hydrolysis at strongly bent regions, momentarily induced by the stirring.
Symons
M C
MC
The Bone and Joint Research Unit St. Bartholomews Hospital School of Medicine London EC1M 6BQ, UK.
D001671:Biochemistry / Q000379:methods*; D003497:Cyclic N-Oxides / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D004249:DNA Damage; D005609:Free Radicals; D009589:Nitrogen Oxides / Q000737:chemistry; D009603:Nitroso Compounds / Q000737:chemistry; D013113:Spin Labels
D003497:Cyclic N-Oxides; D005609:Free Radicals; D009589:Nitrogen Oxides; D009603:Nitroso Compounds; D013113:Spin Labels; C029217:phenyl-N-tert-butylnitrone; C017245:5,5-dimethyl-1-pyrroline-1-oxide; D004247:DNA; C034526:tert-nitrosobutane