[Recombinant human growth hormone downregulates the apoptosis of HepG(2) cells induced by LPS].
OBJECTIVE
To investigate the effect of LPS on human hepatocytes and study whether recombinant human growth hormone (rhGH) could protect hepatocytes from apoptosis induced by LPS.


METHODS
HepG(2) cells were treated with LPS (20 microg/ml) or LPS and rhGH for 16 hours. The apoptosis of HepG(2) cells was detected by terminal deoxynucleotide transferase-mediated dUTP nick end labeling (TUNEL) or electron microscopy.


RESULTS
HepG(2) cells treated with LPS exhibited some specific morphological features of typical apoptosis. the percentage of apoptotic cells in HepG(2) cells treated with LPS and hrGH was significantly lower than that treated with LPS (36+/-5.6)% vs (99+/-0.8)%, P<0.001).


CONCLUSIONS
LPS can induce apoptosis of HepG(2) cells, and hrGH can downregulate the apoptotic role of LPS on HepG(2) cells.
Zhang
D
D
The Institute of Viral Hepatitis, Chongqing University of Medical Sciences, Chongqing 400010, China.
Zhang
D
D
Ren
H
H
Zhou
W
W
D017209:Apoptosis / Q000187:drug effects*; D015536:Down-Regulation; D022781:Hepatocytes / Q000187:drug effects*; D019382:Human Growth Hormone / Q000494:pharmacology*; D006801:Humans; D020287:In Situ Nick-End Labeling; D008070:Lipopolysaccharides / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D008070:Lipopolysaccharides; D011994:Recombinant Proteins; D019382:Human Growth Hormone