Relaxation of activated airway smooth muscle: relative potency of isoproterenol vs. tidal stretch.
Both isoproterenol and tidal fluctuations of muscle length inhibit active force development in activated airway smooth muscle. In this study, we show that length fluctuations in the range of amplitudes expected during quiet tidal breathing produce force inhibition that is equipotent with high concentrations of isoproterenol. Active force fell to 50% of its isometric value when the amplitude of the tidal stretch was 4% of muscle length. The relaxing effects of length fluctuations were insensitive to the specific contractile agonist, suggesting that the mechanism of action is largely independent of the particular signal transduction pathway and lies instead at the level of bridge dynamics. This idea is reinforced by the results of combining the relaxation effects of tidal fluctuations with those produced by isoproterenol at all but the highest concentrations studied (10(-5) M). Such a combination produces multiplicative effects, indicating largely separate modes of action. These observations suggest that the tidal muscle stretches that are attendant to spontaneous breathing comprise the first line of defense against bronchospasm and that tidal muscle stretches may be the most important of all known bronchodilating agencies.
Gump
A
A
Physiology Program, Harvard School of Public Health, Boston, Massachusetts 02130, USA.
Haughney
L
L
Fredberg
J
J
D000818:Animals; D001986:Bronchial Spasm / Q000503:physiopathology; D001993:Bronchodilator Agents / Q000494:pharmacology*; D002417:Cattle; D004305:Dose-Response Relationship, Drug; D066298:In Vitro Techniques; D007537:Isometric Contraction / Q000187:drug effects; D007545:Isoproterenol / Q000494:pharmacology*; D009126:Muscle Relaxation / Q000187:drug effects / Q000502:physiology; D009470:Muscle Spindles / Q000502:physiology*; D009130:Muscle, Smooth / Q000502:physiology*; D011312:Pressure; D012143:Respiratory Physiological Phenomena
D001993:Bronchodilator Agents; D007545:Isoproterenol