title: Prevalence of Prevotella intermedia and Prevotella nigrescens in infected root canals from a Brazilian population.
abstract: The purpose of the present study was to evaluate the prevalence of Prevotella intermedia and Prevotella nigrescens in symptomatic and asymptomatic endodontic infections from a Brazilian population. DNA extracted from samples obtained from 28 cases of endodontic infection were examined by the 16S rDNA-directed Polymerase Chain Reaction (PCR) method. PCR detected P. intermedia in 7.1% of the cases (2 out of 28 teeth) and P. nigrescens in only one sample (3.6%). The low prevalence of P. intermedia and P. nigrescens as reported in the present study is probably justified for geographical reasons.
authors.affiliation: Department of Endodontics, School of Dentistry, Estácio de Sá University, Rio de Janeiro. siqueira@estacio.br
authors.forename: J
authors.initials: J
authors.lastname: Siqueira Júnior
authors.forename: I N
authors.initials: IN
authors.lastname: Rôças
authors.forename: J C
authors.initials: JC
authors.lastname: Oliveira
mesh_terms: D000293:Adolescent; D000328:Adult; D016866:Bacteroidaceae Infections / Q000453:epidemiology*; D001938:Brazil / Q000453:epidemiology; D004269:DNA, Bacterial / Q000032:analysis; D003790:Dental Pulp Necrosis / Q000453:epidemiology / Q000382:microbiology*; D006801:Humans; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D015995:Prevalence; D018720:Prevotella / Q000302:isolation & purification*; D018713:Prevotella intermedia / Q000302:isolation & purification
chemical_list: D004269:DNA, Bacterial