Phenotypic and genotypic characterization of antagonistic bacteria associated with roots of transgenic and non-transgenic potato plants.
Rhizobacteria obtained during a risk assessment study from parental and transgenic T4 lysozyme-expressing potato plants were investigated to determine whether or not the strains could be grouped based on the source of isolation, transgenic or non-transgenic plants, respectively. A total of 68 representative bacterial strains of the group of enterics and pseudomonads were investigated by phenotypic profiling (the antagonistic activity towards bacterial and fungal plant pathogens, the production of the plant growth hormone indole-3-acetic acid [auxin], and the sensitivity to T4 lysozyme in vitro) and genotypic profiling by PCR fingerprints using BOX primers. All isolates were identified by fatty acid methyl ester (FAME) analysis. Computer-based analysis of the phenotypic characteristics showed that both, enterics and Pseudomonas strains clustered into six to seven groups at an Euclidian distance of 10. According to their BOX-PCR-generated fingerprints the Pseudomonas strains clustered into seven groups and the enterobacteria into two groups at the same genetic distance level of 10. The majority of groups were heterogeneous and contained isolates from all plant lines. In conclusion, cluster analysis of the phenotypic and genotypic features did not reveal correlations between bacterial isolates and transgenic character of plants.
Lottmann
J
J
University of Rostock, Department of Biological Science, Institute of Molecular Physiology and Biotechnology, Microbiology, Germany. jl@imppm.bio4.uni-rostock.de
Berg
G
G
D016000:Cluster Analysis; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D004755:Enterobacteriaceae / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D005227:Fatty Acids / Q000032:analysis; D007210:Indoleacetic Acids / Q000032:analysis; D009113:Muramidase / Q000494:pharmacology; D016973:Pectobacterium carotovorum / Q000254:growth & development; D018517:Plant Roots / Q000382:microbiology; D030821:Plants, Genetically Modified / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D011549:Pseudomonas / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D011198:Solanum tuberosum / Q000382:microbiology*; D020172:Verticillium / Q000254:growth & development
D004269:DNA, Bacterial; D005227:Fatty Acids; D007210:Indoleacetic Acids; C030737:indoleacetic acid; D009113:Muramidase