title: Adult-onset alcohol consumption induces osteopenia in female rats.
abstract: BACKGROUND
  Alcohol is a known risk factor for osteopenia and fracture in humans, and its effects on the skeleton have been studied extensively in animal models. Almost all studies of rats, however, have begun rats on alcohol diets while the animals were young and still growing. The purpose of the current study was to examine the effects of alcohol consumption on rats that began drinking alcohol as adults, so that the confounding effects of growth might be minimized.
  
  
  METHODS
  Nine-month-old female Sprague-Dawley rats were studied for two durations (8 and 14 weeks). The following diet groups were used for both durations: alcohol (n = 7), in which rats were fed a liquid diet containing ethanol (8.1% v/v; Lieber-DeCarli method); pair-fed (n = 7), in which rats were fed a caloric-equivalent liquid diet matched to the alcohol-fed animals; and pellet (n = 6), in which rats consumed standard rat chow and water. A cessation protocol was also used in which alcohol- and pair-fed groups were fed liquid diets for 8 weeks and then given pellet chow and water for 6 weeks, with pair feeding maintained during the cessation period.
  
  
  RESULTS
  Only minor effects developed in the rats in the 8-week group, but after 14 weeks, the cancellous bone of the proximal tibia was severely osteopenic in the alcohol-fed animals. The bone volume and trabecular number were both significantly lower in the alcohol-fed animals than in the pair-fed and pellet-fed control animals and also lower than in the alcohol-fed animals in the 8-week group. Mechanical properties of the cancellous bone in the distal femur also were significantly diminished in the 14-week alcohol-fed group. Composition and mechanical properties of the cortical bone in the femur diaphysis were largely unaffected, but the yield stress was significantly lower in the 14-week alcohol-fed group than in the 8-week alcohol-fed group. No significant effects were found in the cessation groups with regard to almost all parameters measured.
  
  
  CONCLUSIONS
  Our study results demonstrate that chronic adult-onset alcohol consumption leads to significantly diminished cancellous bone properties and that these effects depend on the duration of alcohol use.
authors.affiliation: Department of Human Anatomy and Neurobiology, College of Medicine, Texas A&M University, College Station 77843-1114, USA.
authors.forename: H A
authors.initials: HA
authors.lastname: Hogan
authors.forename: F
authors.initials: F
authors.lastname: Argueta
authors.forename: L
authors.initials: L
authors.lastname: Moe
authors.forename: L P
authors.initials: LP
authors.lastname: Nguyen
authors.forename: H W
authors.initials: HW
authors.lastname: Sampson
mesh_terms: D000367:Age Factors; D000428:Alcohol Drinking / Q000009:adverse effects*; D000818:Animals; D001851:Bone Diseases, Metabolic / Q000139:chemically induced*; D002492:Central Nervous System Depressants / Q000009:adverse effects*; D000431:Ethanol / Q000009:adverse effects*; D005260:Female; D051381:Rats; D017207:Rats, Sprague-Dawley; D013977:Tibia / Q000187:drug effects* / Q000378:metabolism
chemical_list: D002492:Central Nervous System Depressants; D000431:Ethanol