title: Radioimmunoassay to detect antitransglutaminase autoantibodies is the most sensitive and specific screening method for celiac disease.
abstract: OBJECTIVE
  The aim of this study was to establish the most sensitive and specific screening method for celiac disease. We tested three methods based on different principles, which all detect autoantibodies against the same antigen (tissue transglutaminase).
  
  
  METHODS
  Sixty-two celiac children at the first biopsy (group 1), 78 celiac children on a gluten-free diet (group 2), 14 celiac children on a gluten-challenge (group 3), and 56 controls with a normal duodenal mucosa (group 4) were studied. The methods used were: 1) radioimmunoprecipitation assay using recombinant tissue transglutaminase (RIA); 2) commercial enzyme immunoassay using guinea pig tissue transglutaminase (ELISA); and 3) indirect immunofluorescence method for detection of antiendomysium antibodies (IF-EMA).
  
  
  RESULTS
  RIA antitransglutaminase autoantibodies were detected in 100% of group 1, 43.6% of group 2, 100% of group 3, and none of the control subjects. ELISA antitransglutaminase autoantibodies were detected in 90.3% of group 1, 9% of group 2, 78.6% of group 3, and in none of the control subjects. IF-EMA were detected in 95.2% of group 1, 11.5% of group 2, 92.3% of group 3, and 1.8% of the controls.
  
  
  CONCLUSIONS
  Our results demonstrate a very high sensitivity and specificity of the RIA method to detect antitransglutaminase autoantibodies in comparison to ELISA and IF-EMA assays. We can explain this finding with the use of human recombinant antigen and the increased capacity of the RIA method to detect low titers of autoantibodies. If our data are confirmed by studies on larger series, tissue transglutaminase RIA could be proposed as the best screening method for celiac patients.
authors.affiliation: Istituto di Clinica Pediatrica, Dipartimento di Scienze Cliniche, Medicina Sperimentale e Patologia, Università La Sapienza, Rome, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Bonamico
authors.forename: C
authors.initials: C
authors.lastname: Tiberti
authors.forename: A
authors.initials: A
authors.lastname: Picarelli
authors.forename: P
authors.initials: P
authors.lastname: Mariani
authors.forename: D
authors.initials: D
authors.lastname: Rossi
authors.forename: E
authors.initials: E
authors.lastname: Cipolletta
authors.forename: M
authors.initials: M
authors.lastname: Greco
authors.forename: M D
authors.initials: MD
authors.lastname: Tola
authors.forename: L
authors.initials: L
authors.lastname: Sabbatella
authors.forename: B
authors.initials: B
authors.lastname: Carabba
authors.forename: F M
authors.initials: FM
authors.lastname: Magliocca
authors.forename: P
authors.initials: P
authors.lastname: Strisciuglio
authors.forename: U
authors.initials: U
authors.lastname: Di Mario
mesh_terms: D000293:Adolescent; D000328:Adult; D001323:Autoantibodies / Q000032:analysis*; D002446:Celiac Disease / Q000175:diagnosis* / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007223:Infant; D008297:Male; D008403:Mass Screening / Q000379:methods*; D009132:Muscles / Q000276:immunology; D011863:Radioimmunoassay / Q000592:standards*; D012680:Sensitivity and Specificity; D011503:Transglutaminases / Q000276:immunology*
chemical_list: D001323:Autoantibodies; D011503:Transglutaminases