[The reaction mechanism between ofloxacin and bovine serum albumin].
OBJECTIVE
To study the reaction mechanism between ofloxacin and bovine serum albumin (BSA) in aqueous solution.


METHODS
Fluorescence spectra and microcalorimetry was used.


RESULTS
The binding constant K was found to be 1.20 x 10(5) L.mol-1 and the number of binding site n was 1.20. Microcalorimetric measurements showed that the molar enthalpy change was delta rHm approximately 0 for the reaction. The effect of ofloxacin on the conformation of BSA was analyzed using synchronous fluorescence spectrometry. The binding distance (r = 2.59 nm) and transfer efficiency (E = 0.38) between ofloxacin and BSA were also obtained according to the theory of Forster's non-radiation energy transfer.


CONCLUSIONS
The interaction between ofloxacin and BSA is stronger and the main binding force is hydrophobic interactions.
Yi
P G
PG
Department of Chemistry, Zhejiang University, Hangzhou 310027, China.
Yu
Q S
QS
Shang
Z C
ZC
Zong
H X
HX
D002151:Calorimetry; D004347:Drug Interactions; D015242:Ofloxacin / Q000737:chemistry / Q000378:metabolism*; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism*; D013050:Spectrometry, Fluorescence
D012710:Serum Albumin, Bovine; D015242:Ofloxacin