Protective effect of lipophilic antioxidants on phorbol-induced acute lung injury in rats.
OBJECTIVE
To determine whether the lipophilic antioxidant U-74389G can ameliorate the acute lung injury induced by phorbol myristate acetate (PMA) in our isolated lung model in rats, and to compare its activity with the intracellular enzymes superoxide dismutase (SOD) or catalase.


METHODS
Randomized, controlled study.


METHODS
Animal-care facility procedure room.


METHODS
Forty-two adult male Sprague-Dawley rats each weighing 250-350 g.


METHODS
Typical acute lung injury was induced successfully by PMA during 60 mins of observation. PMA (2 microg/kg) elicited a significant increase in microvascular permeability (measured by using the capillary filtration coefficient Kfc), lung weight gain, the lung weight/body weight ratio, pulmonary arterial pressure, and the protein concentration of the bronchoalveolar lavage fluid.


RESULTS
Pretreatment with 1 mg of U-74389G significantly attenuated the acute lung injury induced by PMA, all parameters having decreased significantly (p <.001). The protective effect of U-74389G was dose dependent, but SOD (6,000 U/kg) or catalase (50,000 U/kg) exhibited no protective effect.


CONCLUSIONS
U-74389G significantly ameliorates acute lung injury induced by PMA in rats.
Chu
S J
SJ
Department of Emergency Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, Republic of China. d1204812@ndmctsgh.edu.tw
Chang
D M
DM
Wang
D
D
Hsu
K
K
Chiang
C H
CH
D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use*; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D002199:Capillary Permeability / Q000187:drug effects; D002374:Catalase / Q000627:therapeutic use; D004305:Dose-Response Relationship, Drug; D015227:Lipid Peroxidation; D008171:Lung Diseases / Q000139:chemically induced / Q000188:drug therapy*; D008297:Male; D009929:Organ Size / Q000187:drug effects; D011281:Pregnatrienes / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000627:therapeutic use; D013755:Tetradecanoylphorbol Acetate / Q000037:antagonists & inhibitors / Q000633:toxicity*; D007507:Therapeutic Irrigation
D000975:Antioxidants; D011281:Pregnatrienes; C074965:U 74389F; D002374:Catalase; D013482:Superoxide Dismutase; D013755:Tetradecanoylphorbol Acetate