Human CARD12 is a novel CED4/Apaf-1 family member that induces apoptosis.
The CED4/Apaf-1 family of proteins functions as critical regulators of apoptosis and NF-kappaB signaling pathways. A novel human member of this family, called CARD12, was identified that induces apoptosis when expressed in cells. CARD12 is most similar in structure to the CED4/Apaf-1 family member CARD4, and is comprised of an N-terminal caspase recruitment domain (CARD), a central nucleotide-binding site (NBS), and a C-terminal domain of leucine-rich repeats (LRR). The CARD domain of CARD12 interacts selectively with the CARD domain of ASC, a recently identified proapoptotic protein. In addition, CARD12 coprecipitates caspase-1, a caspase that participates in both apoptotic signaling and cytokine processing. CARD12 may assemble with proapoptotic CARD proteins to coordinate the activation of downstream apoptotic and inflammatory signaling pathways.
Geddes
B J
BJ
Millennium Pharmaceuticals, Inc., Cambridge, Massachusetts 02139, USA.
Wang
L
L
Huang
W J
WJ
Lavellee
M
M
Manji
G A
GA
Brown
M
M
Jurman
M
M
Cao
J
J
Morgenstern
J
J
Merriam
S
S
Glucksmann
M A
MA
DiStefano
P S
PS
Bertin
J
J
D000818:Animals; D000918:Antibody Specificity; D017209:Apoptosis; D053477:Apoptotic Protease-Activating Factor 1; D029742:Caenorhabditis elegans Proteins; D002135:Calcium-Binding Proteins / Q000235:genetics*; D020170:Caspase 1 / Q000378:metabolism; D002460:Cell Line; D002522:Chlorocebus aethiops; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D016208:Databases, Factual; D015870:Gene Expression; D017930:Genes, Reporter; D015801:Helminth Proteins / Q000235:genetics*; D006801:Humans; D015151:Immunoblotting; D007668:Kidney / Q000166:cytology / Q000378:metabolism; D008969:Molecular Sequence Data; D005810:Multigene Family; D009928:Organ Specificity; D017434:Protein Structure, Tertiary / Q000502:physiology; D011506:Proteins / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction / Q000502:physiology; D014162:Transfection; D020798:Two-Hybrid System Techniques; D014709:Vero Cells
C506592:APAF1 protein, human; D053477:Apoptotic Protease-Activating Factor 1; D029742:Caenorhabditis elegans Proteins; D002135:Calcium-Binding Proteins; C089224:Ced-4 protein, C elegans; D018076:DNA, Complementary; D015801:Helminth Proteins; D011506:Proteins; D020170:Caspase 1