title: A scanning near-field optical microscope approach to biomolecule patterning.
abstract: In view of future generations of biosensors and advanced biomaterials, photochemistry in the near field using scanning near-field optical microscopy is investigated. The potential of direct near-field-induced photoactivation is demonstrated on standard photoresist. Photoimmobilization of maleimidoaryldiazirine on silicon substrates and bovine serum albumin on glass substrates is achieved, opening the way to a controlled biopatterning of surfaces with submicrometer feature size. The obtained patterns are characterized using atomic force microscopy, time-of-flight secondary ion mass spectroscopy (ToF-SIMS), and near-field fluorescence microscopy.
authors.affiliation: Institut d'Optique Appliquée, Ecole polytechnique Fédérale de Lausanne (EPFL), CH-1015 Lausanne, Switzerland.
authors.forename: C
authors.initials: C
authors.lastname: Philipona
authors.forename: Y
authors.initials: Y
authors.lastname: Chevolot
authors.forename: D
authors.initials: D
authors.lastname: Léonard
authors.forename: H J
authors.initials: HJ
authors.lastname: Mathieu
authors.forename: H
authors.initials: H
authors.lastname: Sigrist
authors.forename: F
authors.initials: F
authors.lastname: Marquis-Weible
mesh_terms: D000327:Adsorption; D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D002417:Cattle; D018625:Microscopy, Atomic Force; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D010777:Photochemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism; D018629:Spectrometry, Mass, Secondary Ion; D013499:Surface Properties; D014159:Transducers
chemical_list: D012710:Serum Albumin, Bovine