title: No background concentrations of di(2-ethylhexyl) phthalate and mono(2-ethylhexyl) phthalate in blood of rats.
abstract: We measured the background levels of di(2-ethylhexyl) phthalate (DEHP) and its hydrolytic metabolite mono(2-ethylhexyl) phthalate (MEHP) in blood from naive female Sprague-Dawley rats and in de-ionized charcoal-purified water using an analytical procedure that is based on sample treatment with acetonitrile, n-hexane extraction and analysis by gas chromatography. In blood, blank values of 91.3 +/- 34.7 micrograms DEHP/l (n = 31) and 30.1 +/- 13.1 micrograms MEHP/l (n = 20) were obtained, and in water, values of 91.6 +/- 44.2 micrograms DEHP/l (n = 26) and 26.7 +/- 10.4 micrograms MEHP/l (n = 15) were found. Since there is no difference between the background valves obtained from blood of naive rats and water, we conclude that DEHP and MEHP result from contamination during the analytical procedure.
authors.affiliation: GSF-Forschungszentrum für Umwelt und Gesundheit, Institut für Toxikologie, Ingolstädter Landstrasse 1, 85764 Neuherberg, Germany.
authors.forename: W
authors.initials: W
authors.lastname: Kessler
authors.forename: W
authors.initials: W
authors.lastname: Phokha
authors.forename: G A
authors.initials: GA
authors.lastname: Csanády
authors.forename: J G
authors.initials: JG
authors.lastname: Filser
mesh_terms: D000818:Animals; D002849:Chromatography, Gas; D004051:Diethylhexyl Phthalate / Q000031:analogs & derivatives / Q000097:blood*; D005260:Female; D051381:Rats; D017207:Rats, Sprague-Dawley; D014867:Water / Q000032:analysis
chemical_list: D014867:Water; D004051:Diethylhexyl Phthalate; C016599:mono-(2-ethylhexyl)phthalate