The role of IL-5, IL-6 and IL-10 in primary and vaccine-primed immune responses to infection with Friend retrovirus (Murine leukaemia virus).
The defence of a host against viral infections is strongly influenced by cytokines. We investigated the role of the B-cell stimulating cytokines IL-5 and IL-6, and the immuno-suppressive cytokine IL-10, during primary and secondary immune responses in mice against infection with Friend retrovirus (FV) (Murine leukaemia virus). IL-5(-/-) mice were comparable to C57BL/6 wild-type mice in their ability to control acute FV infection. In contrast, IL-6(-/-) and IL-10(-/-) mice showed significantly enhanced virus loads in spleen cells. However, this impaired control of acute FV replication did not alter the long-term control over persistent FV in IL-6(-/-) and IL-10(-/-) mice. Immunization with a live attenuated vaccine virus prior to challenge protected all three types of cytokine-deficient mice from high levels of spleen virus, despite the finding that the vaccinated IL-5- and IL-6-deficient mice had significantly reduced titres of virus-neutralizing IgG class antibodies. The results indicate that IL-6 and IL-10 contribute to primary immune responses against FV, but are dispensable during persistent infection and vaccine-primed secondary responses.
Strestik
Beatrice D
BD
Institut für Virologie der Universität Würzburg, Versbacher Str.7, 97078 Würzburg, Germany1.
Olbrich
Anke R M
ARM
Institut für Virologie der Universität Würzburg, Versbacher Str.7, 97078 Würzburg, Germany1.
Hasenkrug
Kim J
KJ
Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, MT 59840, Hamilton, USA2.
Dittmer
Ulf
U
Institut für Virologie der Universität Würzburg, Versbacher Str.7, 97078 Würzburg, Germany1.
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D005622:Friend murine leukemia virus / Q000276:immunology* / Q000502:physiology; D017353:Gene Deletion; D007074:Immunoglobulin G / Q000276:immunology; D016753:Interleukin-10 / Q000172:deficiency / Q000235:genetics / Q000276:immunology*; D015848:Interleukin-5 / Q000172:deficiency / Q000235:genetics / Q000276:immunology*; D015850:Interleukin-6 / Q000172:deficiency / Q000235:genetics / Q000276:immunology*; D007942:Leukemia, Experimental / Q000276:immunology* / Q000517:prevention & control / Q000821:virology; D051379:Mice; D008810:Mice, Inbred C57BL; D009500:Neutralization Tests; D012192:Retroviridae Infections / Q000276:immunology / Q000821:virology; D013154:Spleen / Q000276:immunology / Q000821:virology; D014412:Tumor Virus Infections / Q000276:immunology / Q000821:virology; D014611:Vaccination; D014613:Vaccines, Attenuated / Q000276:immunology; D019562:Viral Load; D014765:Viral Vaccines / Q000276:immunology*; D014779:Virus Replication
D000914:Antibodies, Viral; D007074:Immunoglobulin G; D015848:Interleukin-5; D015850:Interleukin-6; D014613:Vaccines, Attenuated; D014765:Viral Vaccines; D016753:Interleukin-10