Quantitative PCR method for diagnosis of citrus bacterial canker.
For diagnosis of citrus bacterial canker by PCR, an internal standard is employed to ensure the quality of the DNA extraction and that proper requisites exist for the amplification reaction. The ratio of PCR products from the internal standard and bacterial target is used to estimate the initial bacterial concentration in citrus tissues with lesions.
Cubero
J
J
University of Florida, Citrus Research and Education Center, Lake Alfred, Florida 33850, USA.
Graham
J H
JH
Gottwald
T R
TR
D002957:Citrus / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D010935:Plant Diseases / Q000382:microbiology*; D018515:Plant Leaves / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000592:standards; D014974:Xanthomonas / Q000235:genetics* / Q000302:isolation & purification*
D004269:DNA, Bacterial