title: Diverse transcriptional initiation revealed by fine, large-scale mapping of mRNA start sites.
abstract: Determination of the mRNA start site is the first step in identifying the promoter region, which is of key importance for transcriptional regulation of gene expression. The 'oligo-capping' method enabled us to introduce a sequence tag to the first base of an mRNA by replacing the cap structure of the mRNA. Using cDNA libraries made from oligo-capped mRNAs, we could identify the transcriptional start site of an individual mRNA just by sequencing the 5'-end of the cDNA. The fine mapping of transcriptional start sites was performed for 5880 mRNAs in 276 human genes. Contrary to our expectations, the majority of the genes showed a diverse distribution of transcriptional start sites. They were distributed over 61.7 bp with a standard deviation of 19.5. Our finding may reflect the dynamic nature of transcriptional initiation events of human genes in vivo.
authors.affiliation: Department of Virology, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan. ysuzuki@manage.ims.u-tokyo.ac.jp
authors.forename: Y
authors.initials: Y
authors.lastname: Suzuki
authors.forename: H
authors.initials: H
authors.lastname: Taira
authors.forename: T
authors.initials: T
authors.lastname: Tsunoda
authors.forename: J
authors.initials: J
authors.lastname: Mizushima-Sugano
authors.forename: J
authors.initials: J
authors.lastname: Sese
authors.forename: H
authors.initials: H
authors.lastname: Hata
authors.forename: T
authors.initials: T
authors.lastname: Ota
authors.forename: T
authors.initials: T
authors.lastname: Isogai
authors.forename: T
authors.initials: T
authors.lastname: Tanaka
authors.forename: S
authors.initials: S
authors.lastname: Morishita
authors.forename: K
authors.initials: K
authors.lastname: Okubo
authors.forename: Y
authors.initials: Y
authors.lastname: Sakaki
authors.forename: Y
authors.initials: Y
authors.lastname: Nakamura
authors.forename: A
authors.initials: A
authors.lastname: Suyama
authors.forename: S
authors.initials: S
authors.lastname: Sugano
mesh_terms: D001483:Base Sequence; D016208:Databases, Factual; D005786:Gene Expression Regulation; D015723:Gene Library; D006801:Humans; D008969:Molecular Sequence Data; D009843:Oligoribonucleotides / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012315:RNA Caps; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D016324:Sequence Tagged Sites; D014158:Transcription, Genetic
chemical_list: D009843:Oligoribonucleotides; D012315:RNA Caps; D012333:RNA, Messenger