Involvement of reactive oxygen species in the induction of (S)-N-p-coumaroyloctopamine accumulation by beta-1,3-glucooligosaccharide elicitors in potato tuber tissues.
Treatment of potato tuber tissues with beta-1,3-glucooligosaccharide induces accumulation of (S)-N-p-coumaroyloctopamine (p-CO). We examined the role of reactive oxygen species (ROS) and nitric oxide (NO) in the signal transduction leading to p-CO accumulation. Induction was suppressed by an NADPH-oxidase inhibitor, diphenyleneiodonium chloride, and oxygen radical scavengers. H2O2 was generated in the tuber tissue within a few minutes of treatment with beta-1,3-glucooligosaccharide. On the other hand, treatment with NO specific scavenger, nitric oxide synthase inhibitor, and serine protease inhibitor did not inhibit p-CO induction. Our findings suggest that ROS generated by the action of NADPH-oxidase play an important role in this system, while NO and serine protease are unlikely to be involved in this process.
Matsuda
F
F
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Japan. matsuda@colorado.kais.kyoto-u.ac.jp
Miyagawa
H
H
Ueno
T
T
D014013:1,2-Dihydroxybenzene-3,5-Disulfonic Acid Disodium Salt / Q000494:pharmacology; D003373:Coumaric Acids / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005936:Glucans; D005947:Glucose; D006861:Hydrogen Peroxide / Q000378:metabolism; D019255:NADPH Oxidases / Q000378:metabolism; D009655:Octopamine / Q000031:analogs & derivatives* / Q000378:metabolism*; D009844:Oligosaccharides / Q000494:pharmacology*; D009861:Onium Compounds / Q000494:pharmacology; D010650:Phenylalanine Ammonia-Lyase / Q000378:metabolism; D011134:Polysaccharides / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism*; D015398:Signal Transduction; D011198:Solanum tuberosum / Q000187:drug effects / Q000378:metabolism*; D014445:Tyrosine Decarboxylase / Q000378:metabolism
D003373:Coumaric Acids; D004791:Enzyme Inhibitors; D005936:Glucans; C410948:N-4-coumaroyloctopamine; D009844:Oligosaccharides; D009861:Onium Compounds; D011134:Polysaccharides; D017382:Reactive Oxygen Species; D009655:Octopamine; D014013:1,2-Dihydroxybenzene-3,5-Disulfonic Acid Disodium Salt; C007517:diphenyleneiodonium; C008247:laminaran; D006861:Hydrogen Peroxide; D019255:NADPH Oxidases; D014445:Tyrosine Decarboxylase; D010650:Phenylalanine Ammonia-Lyase; D005947:Glucose