Protein kinase C-alpha(1b)-adrenoceptor coimmunoprecipitation: effect of hormones and phorbol myristate acetate.
alpha(1b)-Adrenoceptors immunoprecipitated with protein kinase C alpha, delta, and epsilon isoforms under basal conditions and such coimmunoprecipitations were increased in cells treated with phorbol myristate acetate. The increased coimmunoprecipitations induced by phorbol myristate acetate were concentration-dependent and reached their maxima 1 to 2 min after the addition of the tumor promoter. No coimmunoprecipitation of protein kinase C zeta and alpha(1b)-adrenoceptors was detected. Norepinephrine, endothelin-1, lysophosphatidic acid and epidermal growth factor were also able to increase the coimmunoprecipitation of protein kinase C isoenzymes and alpha(1b)-adrenoceptors. These data support the idea that protein kinase-receptor complexes might form and could be relevant in receptor desensitization.
Alcántara-Hernández
R
R
Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Apartado Postal 70-248, D.F. 04510, Mexico.
Leyva-Illades
D
D
García-Sáinz
J A
JA
D000316:Adrenergic alpha-Agonists / Q000494:pharmacology; D000818:Animals; D002460:Cell Line; D019332:Endothelin-1 / Q000494:pharmacology; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005347:Fibroblasts; D006728:Hormones / Q000494:pharmacology*; D015151:Immunoblotting; D007527:Isoenzymes / Q000378:metabolism*; D008246:Lysophospholipids / Q000494:pharmacology; D009638:Norepinephrine / Q000494:pharmacology; D011233:Precipitin Tests; D011493:Protein Kinase C / Q000378:metabolism*; D051571:Protein Kinase C-alpha; D051381:Rats; D018340:Receptors, Adrenergic, alpha-1 / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D000316:Adrenergic alpha-Agonists; D019332:Endothelin-1; D006728:Hormones; D007527:Isoenzymes; D008246:Lysophospholipids; D018340:Receptors, Adrenergic, alpha-1; D004815:Epidermal Growth Factor; D011493:Protein Kinase C; D051571:Protein Kinase C-alpha; D013755:Tetradecanoylphorbol Acetate; D009638:Norepinephrine