On-line sample preparation for high throughput reversed-phase LC/MS analysis of combinatorial chemistry libraries.
An on-line sample preparation method utilizing a time-programmed autosampler is described for high throughput liquid chromatography/mass spectrometry (LC/MS). This approach is particularly helpful for the LC/MS analysis of samples which require solvents incompatible with HPLC in the sample preparation process. The on-line sample preparation approach minimizes a bottleneck in throughput and improves sample recovery under some circumstances.
Tang
L
L
Affymax Research Institute, 4001 Miranda Avenue, Palo Alto, CA 94304, USA. ltang@tularik.com
Fitch
W L
WL
Smith
P
P
Tumelty
D
D
Cao
K
K
Ferla
S W
SW
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D020650:Combinatorial Chemistry Techniques; D013058:Mass Spectrometry / Q000379:methods*; D009862:Online Systems