Preliminary immunohistochemical investigations of human thyroid C cells in the simple and hyperactive nodular goitre.
The aim of this study was to carry out histomorphological and immunohistochemical analysis of thyroid C cells in 30 patients with simple goitre and hyperactive goitre including Graves-Basedow (G-B) disease, treated surgically. Four tissue samples were always taken from the same internal parts of the gland where the number of C cells in physiological conditions was the highest. C cells were detected in paraffin sections after impregnation with silver salt (Grimelius method) or immunohistochemically, with antibodies against calcitonin, synaptophysin, chromogranin A and neuron-specific enolase. Distinctly less numerous C cells were found in simple and hyperactive goitre than in normal thyroid parenchyma. The majority of C cells showed weak intensity of the examined immunohistochemical reactions. C cells were not observed at all in the texture of nodules well separated by the connective tissue. Proliferative changes concerned only follicular cells.
Dadan
J
J
1st Department of General Surgery, Medical Academy, Białystok, Poland. klchirog@amb.ac.bialystok.pl
Sawicki
B
B
Chyczewski
L
L
Azzadin
A
A
Malla
H
H
Dziecioł
J
J
Puchalski
Z
Z
D000328:Adult; D000368:Aged; D005260:Female; D006044:Goiter, Nodular / Q000473:pathology* / Q000601:surgery; D006111:Graves Disease / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D013961:Thyroid Gland / Q000473:pathology* / Q000601:surgery