In vitro taurocholate-induced segmentation and clustering of Mesocestoides vogae (syn. corti) tetrathyridia (Cestoda)--inhibition byh cestocidal drugs.
Mesocestoides vogae (syn. M. corti) tetrathyridia were cultured in the presence of sodium taurocholate, for the purpose of exploring the suitability of this organism for the in vitro assay of cestocidal drugs. Parasite clustering and segmentation were observed as taurocholate-dependent effects in biphasic and monophasic media, respectively. Interestingly, representative members of two major classes of known cestocidal agents (namely, albendazole and praziquantel) blocked these effects. Furthermore, it was possible to determine a specific concentration of the drugs that inhibited clustering and segmentation (minimum inhibitory concentration). In contrast, no inhibition was obtained in the presence of anthelmintics without cestocidal activity. These observations open the way for further studies focused at understanding how the activity of the drugs is involved in the suppression of the taurocholate-induced effects.
Saldaña
J
J
Departamento de Farmacia, Facultad de Química, Universidad de la República, Montevideo, Uruguay.
Marín
M
M
Fernández
C
C
Domínguez
L
L
D000818:Animals; D000923:Anticestodal Agents / Q000494:pharmacology*; D003470:Culture Media; D008297:Male; D008645:Mesocestoides / Q000187:drug effects* / Q000254:growth & development / Q000502:physiology; D051379:Mice; D013656:Taurocholic Acid / Q000494:pharmacology*
D000923:Anticestodal Agents; D003470:Culture Media; D013656:Taurocholic Acid