Isolation of novel heart-specific genes using the BodyMap database.
Two novel heart-specific genes, C3orf3 (chromosome 3 open reading frame 3) and MMGL (myomegalin-like), were isolated using BodyMap, a gene expression database based on site-directed 3' expressed sequence tags (3'-ESTs) which were collected from nonbiased cDNA libraries of various tissues. The cDNA of C3orf3 was 1667 bp and was composed of 12 exons within a 10-kb-long genomic sequence. MMGL consisted of 8 exons within a genomic sequence of over 70 kb, leading to four alternatively spliced transcripts. Both genes were strongly expressed in heart and also in skeletal muscle. C3orf3 and MMGL were mapped to 3p22 and 1q1, respectively. Subcellular localizations of their putative proteins were determined as being in the cytoplasm for C3orf3 and in the cytoplasm and nucleus for MMGL. This study showed that BodyMap is a useful database for the isolation of tissue-specific genes.
Soejima
H
H
Department of Biochemistry, Saga Medical School, 5-1-1 Nabeshima, Saga, 849-8501, Japan. soejimah@post.saga-med.ac.jp
Kawamoto
S
S
Akai
J
J
Miyoshi
O
O
Arai
Y
Y
Morohka
T
T
Matsuo
S
S
Niikawa
N
N
Kimura
A
A
Okubo
K
K
Mukai
T
T
D048868:Adaptor Proteins, Signal Transducing; D017398:Alternative Splicing; D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D002874:Chromosome Mapping; D002878:Chromosomes, Human, Pair 1 / Q000235:genetics; D002893:Chromosomes, Human, Pair 3 / Q000235:genetics; D003598:Cytoskeletal Proteins; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D016208:Databases, Factual; D005091:Exons; D020224:Expressed Sequence Tags; D005260:Female; D015723:Gene Library; D005796:Genes / Q000235:genetics*; D006801:Humans; D007438:Introns; D008565:Membrane Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D009124:Muscle Proteins; D009206:Myocardium / Q000378:metabolism*; D009687:Nuclear Proteins / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D014018:Tissue Distribution
D048868:Adaptor Proteins, Signal Transducing; D003598:Cytoskeletal Proteins; D018076:DNA, Complementary; C432970:HHATL protein, human; D008565:Membrane Proteins; D009124:Muscle Proteins; D009687:Nuclear Proteins; C432971:PDE4DIP protein, human; D012333:RNA, Messenger