Controlling self-incompatibility by CO2 gas treatment in Brassica campestris: structural alteration of papillae cell and differential gene expression by increased CO2 gas.
Self-imcompatibility is a controlling genetic mechanism to prevent self-pollination for Chinese cabbage (Brassica campestris), one of the major vegetable crops in Korea. To maintain inbred lines of the crop plant, a method in that high CO2 gas is treated to the pistils to overcome the self-incompatibility and thereby self-pollens can successfully make germination and fertilization has been widely used in seed companies. Despite the common utilization of this method, any molecular and cellular studies on how the self-incompatibility is removed from the Chinese cabbage plant have not been done. In this study, we show that the increased CO2 gas causes a structural alteration of the papillae cell and thereby the self-incompatibility is removed from the Chinese cabbage plant, allowing the self-pollens to germinate and penetrate the papillae cell. Also, gene expression in the pistil treated with CO2 gas was studied by DD/RT-PCR and reverse northern hybridization experiments. The results suggest that the failure in self-incompatible reaction resulted not only from the structural alteration of the papillae cell but also from change in the pistil component production that is either positively or negatively regulated by the environmental stimulation.
Lee
S H
SH
Department of Biology, Korea University, Seoul.
Hong
M Y
MY
Kim
S
S
Lee
J S
JS
Kim
B D
BD
Min
B H
BH
Baek
N K
NK
Chung
Y Y
YY
D015152:Blotting, Northern; D001937:Brassica / Q000187:drug effects / Q000235:genetics / Q000502:physiology* / Q000648:ultrastructure; D002245:Carbon Dioxide / Q000494:pharmacology*; D018556:Crops, Agricultural / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D010940:Plant Proteins / Q000378:metabolism; D012098:Reproduction; D020133:Reverse Transcriptase Polymerase Chain Reaction
D010940:Plant Proteins; D002245:Carbon Dioxide