title: Quinolones alter defense reactions mediated by macrophages.
abstract: The innate host defense system is regulated in part by the number and activation of the function of neutrophils and macrophages. The immunological effects of a variety of quinolones on host defense functions of macrophage are reported. Ofloxacin (OFLX), lomefloxacin (LFLX), tosufloxacin (TFLX), fleroxacin (FLRX), sparfloxacin (SPFX) and levofloxacin (LVFX) significantly inhibited phagocytosis of Escherichica coli by macrophages. Moreover, TFLX markedly potentiated the expression of the adhesion molecule Mac-1 by macrophages. No significant alteration was detected in the adherence and the expression of adhesion molecule Mac-1 in macrophages treated with the other quinolones. In contrast, OFLX, LFLX, TFLX, and LVFX were effective in significantly increasing the production of hydrogen peroxide, while the other agents did not. These results suggest that the quinolones at a therapeutic concentration differentially affect phagocytosis, adhesion, and the production of hydrogen peroxide by macrophages.
authors.affiliation: Department of Pharmacology, Osaka Dental University, Hirakata, Japan. aazuma@cc.osaka-dent.ac.jp
authors.forename: Y
authors.initials: Y
authors.lastname: Azuma
authors.forename: M
authors.initials: M
authors.lastname: Shinohara
authors.forename: P L
authors.initials: PL
authors.lastname: Wang
authors.forename: K
authors.initials: K
authors.lastname: Ohura
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D024841:Fluoroquinolones; D006861:Hydrogen Peroxide / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects* / Q000502:physiology; D008297:Male; D010587:Phagocytosis / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D013481:Superoxides / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D000890:Anti-Infective Agents; D024841:Fluoroquinolones; D013481:Superoxides; D006861:Hydrogen Peroxide; D013755:Tetradecanoylphorbol Acetate