title: Infectivity of cysts of the ME-49 Toxoplasma gondii strain in bovine milk and homemade cheese.
abstract: OBJECTIVE
  Analyze the infectivity and storage resistance of cysts of the ME-49 strain of Toxoplasma gondii in artificially infected bovine milk and homemade fresh cheese.
  
  
  METHODS
  Pasteurized bovine milk was infected with 10 cysts/ml of the ME-49 strain of T.gondii and inoculated in different groups of mice, immediately or after storage at 4 degrees C for 5, 10 and 20 days. Homemade fresh cheese was prepared with artificially infected milk, and also tested in groups of mice, using the same storage process. Infection was identified by the presence of cysts in the brain or serological testing in challenged mice after 5 weeks, confirmed by Western Blot and histology.
  
  
  RESULTS
  The infectivity of cysts of the ME-49 strain of T.gondii was maintained in the milk even after storage for 20 days at refrigerator temperatures. Cysts were also able to survive the production process of homemade fresh cheese and storage for a period of 10 days in the same conditions.
  
  
  CONCLUSIONS
  These data demonstrated that milk and dairy products could be an important source of T.gondii in human contamination, reinforcing the importance of milk pasteurization before any processing or ingestion.
authors.affiliation: Laboratório de Protozoologia, Instituto de Medicina Tropical, Faculdade de Medicina, Universidade de São Paulo, São Paulo, SP, Brasil.
authors.forename: R M
authors.initials: RM
authors.lastname: Hiramoto
authors.forename: M
authors.initials: M
authors.lastname: Mayrbaurl-Borges
authors.forename: A J
authors.initials: AJ
authors.lastname: Galisteo
authors.forename: L R
authors.initials: LR
authors.lastname: Meireles
authors.forename: M S
authors.initials: MS
authors.lastname: Macre
authors.forename: H F
authors.initials: HF
authors.lastname: Andrade
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002611:Cheese / Q000469:parasitology*; D004797:Enzyme-Linked Immunosorbent Assay; D016266:Food Parasitology; D051379:Mice; D008810:Mice, Inbred C57BL; D008892:Milk / Q000469:parasitology*; D013170:Spores / Q000502:physiology*; D013997:Time Factors; D014122:Toxoplasma / Q000276:immunology / Q000472:pathogenicity*; D014123:Toxoplasmosis / Q000401:mortality / Q000635:transmission*