title: Effects of external beam irradiation on the TRAM flap: an experimental model.
abstract: The rat model of the transverse rectus abdominis musculocutaneous (TRAM) flap was used in the present study to determine the effects of external beam radiation on myocutaneous flap histology and pathophysiology. A total of 57 adult Sprague-Dawley rats underwent a TRAM procedure. A pilot study with 17 animals was first performed to determine proper radiation dosages, and the remaining 40 rats were then used in the definitive study. In half of the definitive study group, the flaps were subjected to fractionated doses of external beam radiation, whereas the other half served as controls. Six weeks after the last radiation dose, all animals were killed and the flaps were harvested for mechanical assessment and histopathologic evaluation. All TRAM flaps survived in both groups. The irradiated and nonirradiated flaps were minimally distinguishable in viscoelastic properties, as well as by histopathologic examination. Growth of the flap in the irradiated animals was significantly diminished (48 percent average surface area increase in irradiated flaps, versus 92 percent increase in nonirradiated flaps, p < 0.05). These findings suggest that the myocutaneous flap is relatively resistant to some of the known adverse affects of radiation on living tissues.
authors.affiliation: Department of Plastic Surgery, Box 800376, University of Virginia Health Sciences Center, Charlottesville, VA 22908, USA. kyl5s@virginia.edu
authors.forename: K Y
authors.initials: KY
authors.lastname: Lin
authors.forename: J W
authors.initials: JW
authors.lastname: Patterson
authors.forename: J
authors.initials: J
authors.lastname: Simmons
authors.forename: M D
authors.initials: MD
authors.lastname: Long
authors.forename: R O
authors.initials: RO
authors.lastname: Schultz
authors.forename: L R
authors.initials: LR
authors.lastname: Amiss
authors.forename: J A
authors.initials: JA
authors.lastname: Molloy
authors.forename: M D
authors.initials: MD
authors.lastname: Kelly
mesh_terms: D000818:Animals; D005260:Female; D011829:Radiation Dosage; D011833:Radiation Injuries, Experimental / Q000473:pathology*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013524:Surgical Flaps