title: Identification and quantitation of sodium-thyroxine and its degradation products by LC using electrochemical and MS detection.
abstract: High performance liquid chromatography (HPLC) was used in combination with an amperometric and mass spectrometric detection to elucidate and quantitate the degradation products and contaminants of the photo-sensitive Na-thyroxine. Using HPLC with amperometric detection, seven decomposition compounds were separated. These products, which occur mostly as contaminants, were then identified by a developed liquid chromatography-mass spectrometry technique. The same HPLC method was also employed to analyze Na-thyroxine and its degradation products in three commercially available brands of Na-thyroxine tablets.
authors.affiliation: College of Pharmacy, Medical Sciences University of Tehran, Tehran, Iran. kazemifa@sina.tums.ac.ir
authors.forename: A G
authors.initials: AG
authors.lastname: Kazemifard
authors.forename: D E
authors.initials: DE
authors.lastname: Moore
authors.forename: A
authors.initials: A
authors.lastname: Aghazadeh
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004563:Electrochemistry; D013058:Mass Spectrometry; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013974:Thyroxine / Q000032:analysis*
chemical_list: D013974:Thyroxine