The ORFO product of Potato leafroll virus is indispensable for virus accumulation.
Using a cDNA expression cassette in combination with agroinoculation of potato leaf discs we have investigated the role the protein encoded by ORF0 of Potato leafroll virus (PLRV) and have shown its importance for virus accumulation. Two mutations introduced into ORF0 by site-directed mutagenesis prevented expression of the corresponding protein and completely abolished virus accumulation in plant cells. They did not, however, affect translation of ORF1 and ORF2. We therefore conclude that ORF0 of PLRV produces a protein essential for virus accumulation, a hitherto undescribed finding.
Sadowy
Ewa
E
Institut des Sciences Végétales CNRS, Av. de la Terrasse, 91 198 Gif-sur-Yvette, France2.|Institute of Biochemistry and Biophysics PAS, Ul. Pawińskiego 5A, 02-106 Warsaw, Poland1.
Maasen
Anna
A
Institute of Biochemistry and Biophysics PAS, Ul. Pawińskiego 5A, 02-106 Warsaw, Poland1.
Juszczuk
Marek
M
Institute of Biochemistry and Biophysics PAS, Ul. Pawińskiego 5A, 02-106 Warsaw, Poland1.
David
Chantal
C
Institut des Sciences Végétales CNRS, Av. de la Terrasse, 91 198 Gif-sur-Yvette, France2.
Zagórski-Ostoja
Wlodzimierz
W
Institute of Biochemistry and Biophysics PAS, Ul. Pawińskiego 5A, 02-106 Warsaw, Poland1.
Gronenborn
Bruno
B
Institut des Sciences Végétales CNRS, Av. de la Terrasse, 91 198 Gif-sur-Yvette, France2.
Hulanicka
M Danuta
MD
Institute of Biochemistry and Biophysics PAS, Ul. Pawińskiego 5A, 02-106 Warsaw, Poland1.
D020043:Genes, Essential / Q000235:genetics; D017793:Luteovirus / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D016297:Mutagenesis, Site-Directed / Q000235:genetics; D016366:Open Reading Frames / Q000235:genetics; D018515:Plant Leaves / Q000166:cytology / Q000821:virology; D014176:Protein Biosynthesis; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism; D011198:Solanum tuberosum / Q000166:cytology / Q000821:virology*; D014764:Viral Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D014779:Virus Replication / Q000235:genetics
D012367:RNA, Viral; D014764:Viral Proteins