Induction of sigma(B)-dependent general stress genes by amino acid starvation in a spo0H mutant of Bacillus subtilis.
Solely sigma(B)-dependent genes like gsiB and gspA are not significantly induced in amino acid-starved wild-type cells, since amino acid starvation does not trigger activation of sigma(B). The general stress gene yvyD is subject to the control of both sigma(B) and sigma(H) therefore displaying induction in response to amino acid starvation at the sigma(H)-dependent promoter. Surprisingly, the proteins YvyD, GsiB and GspA were significantly induced in amino acid-starved cells of a strain lacking sigma(H) activity. Transcriptional studies provided evidence that sigma(B)-dependent transcription is indeed induced in a spo0H mutant during amino acid starvation and depends on RsbP but not on RsbU indicating that the stress signal transduction is not required for this induction. A similar phenomenon of sigma(B) activation was observed in amino acid-starved cells of a spo0A deletion mutant. The sigma(B)-dependent transcription in a spo0H mutant further needs an active RelA protein which is responsible for strong repression of house-keeping genes after amino acid starvation (stringent response). Our data indicate that in the absence of sigma(H) and under conditions which provoke the stringent response, RsbP-dependent levels of active sigma(B) can more effectively compete for increased levels of free RNA polymerase core enzyme leading to the induction of the probably strongest sigma(B)-dependent genes.
Eymann
C
C
Institut für Mikrobiologie, Ernst-Moritz-Arndt-Universität Greifswald, F.-L.-Jahnstrasse 15, D-17487, Greifswald, Germany.
Hecker
M
M
D000596:Amino Acids / Q000172:deficiency / Q000378:metabolism*; D001412:Bacillus subtilis / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics; D005786:Gene Expression Regulation; D006360:Heat-Shock Proteins / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis; D012808:Sigma Factor / Q000096:biosynthesis* / Q000235:genetics
D000596:Amino Acids; D001426:Bacterial Proteins; D006360:Heat-Shock Proteins; D012333:RNA, Messenger; C079011:SigB protein, Bacteria; C120328:SigH protein, bacteria; D012808:Sigma Factor; C116647:yvyD protein, Bacillus subtilis