title: Increased proteolytic activity and matrix metalloprotease expression in lungs during infection by porcine reproductive and respiratory syndrome virus.
abstract: The local increase in the secretion of extracellular proteases, allowing cleavage of the extracellular matrix and thereby facilitating the infiltration of T cells, monocytes and neutrophils, is a hallmark of chronic inflammation and autoimmunity. In pulmonary genetic diseases, such as emphysema and cystic fibrosis, proteases can also favour the development of local immunodeficiency by degrading key regulators of the immune response, such as CD4, CD8, IgG, ICAM-1 and C3b receptors. Since several infectious agents can give rise to severe pulmonary disorders associated with opportunistic infections, we sought to determine whether an increase in proteolytic activity occurred during infection with porcine reproductive and respiratory syndrome virus (PRRSV), the causative agent of a new disease in swine characterized by severe respiratory problems in young pigs. Piglets were infected with the virus and bronchoalveolar lavages were collected at various times post-infection to measure the net proteolytic activity. It was shown that PRRSV infection leads to a significant increase in proteolytic activity in pulmonary fluids. Maximal activity was found at 7 and 14 days post-infection, with a return towards normal levels at day 42. Zymographic analyses showed a significant increase in the secretion of matrix metalloproteases (MMPs) 2 and 9, two enzymes involved in tissue remodelling. Histological analyses showed a correlation between the increase in proteolytic activity and the appearance of lesions that were characterized by massive lymphomononuclear cell infiltration. These results suggest that virus infection of the lungs can lead to a transient increase in proteolytic activity that could favour opportunistic infection.
authors.affiliation: Centre de Recherche en Santé Humaine1 et Centre de Recherche en Microbiologie et Biotechnologie2, INRS-Institut Armand-Frappier, 531 boul. des Prairies, Laval, Laval-des-Rapides, Québec, CanadaH7N 4Z3.
authors.forename: Manon
authors.initials: M
authors.lastname: Girard
authors.affiliation: Centre de Recherche en Santé Humaine1 et Centre de Recherche en Microbiologie et Biotechnologie2, INRS-Institut Armand-Frappier, 531 boul. des Prairies, Laval, Laval-des-Rapides, Québec, CanadaH7N 4Z3.
authors.forename: Patrick
authors.initials: P
authors.lastname: Cléroux
authors.affiliation: Centre de Recherche en Santé Humaine1 et Centre de Recherche en Microbiologie et Biotechnologie2, INRS-Institut Armand-Frappier, 531 boul. des Prairies, Laval, Laval-des-Rapides, Québec, CanadaH7N 4Z3.
authors.forename: Pierre
authors.initials: P
authors.lastname: Tremblay
authors.affiliation: Centre de Recherche en Santé Humaine1 et Centre de Recherche en Microbiologie et Biotechnologie2, INRS-Institut Armand-Frappier, 531 boul. des Prairies, Laval, Laval-des-Rapides, Québec, CanadaH7N 4Z3.
authors.forename: Serge
authors.initials: S
authors.lastname: Dea
authors.affiliation: Centre de Recherche en Santé Humaine1 et Centre de Recherche en Microbiologie et Biotechnologie2, INRS-Institut Armand-Frappier, 531 boul. des Prairies, Laval, Laval-des-Rapides, Québec, CanadaH7N 4Z3.
authors.forename: Yves
authors.initials: Y
authors.lastname: St-Pierre
mesh_terms: D000818:Animals; D018893:Bronchoalveolar Lavage; D003094:Collagen / Q000378:metabolism; D004790:Enzyme Induction; D016679:Genome, Viral; D007249:Inflammation / Q000201:enzymology / Q000378:metabolism / Q000473:pathology / Q000821:virology; D008168:Lung / Q000201:enzymology* / Q000378:metabolism / Q000473:pathology / Q000821:virology; D020782:Matrix Metalloproteinases / Q000097:blood / Q000378:metabolism*; D019318:Porcine Reproductive and Respiratory Syndrome / Q000201:enzymology* / Q000378:metabolism / Q000473:pathology; D019316:Porcine respiratory and reproductive syndrome virus / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D012367:RNA, Viral / Q000032:analysis; D013154:Spleen / Q000821:virology; D013552:Swine / Q000821:virology*
chemical_list: D012367:RNA, Viral; D003094:Collagen; D020782:Matrix Metalloproteinases