[Herbal compound 861 inhibits NF-kappa B binding activity of hepatic stellate cells in vitro].
OBJECTIVE
To investigate the effect of Cpd 861 on nuclear factor-kappa B (NF-kappa B) binding activity of hepatic stellate cells (HSC) in vitro.


METHODS
The study was carried out on the culture of hepatic stellate cell line, 5mg/ml of Cpd 861 was added and incubated for 48 hours. NF-kappa B binding activity was evaluated by electrophoretic mobility shift assays. IL-6 and sICAM-1 levels in the cultured supernatant were detected by ELISA. Cell apoptosis was detected by flow cytometry and TUNEL.


RESULTS
Cpd 861 suppressed the binding activity of NF-kappa B in HSCs compared with the control group. Moreover, IL-6 and sICAM-1 levels in the cultured supernatant were decreased (P<0.05) and apoptosis rate of HSCs was increased after Cpd 861 incubation (P<0.01).


CONCLUSIONS
The inhibitory effect on NF-kappa B binding activity might be part of the cellular mechanism of Cpd 861 to treat liver fibrosis.
You
H
H
Beijing Friendship Hospital, Capital University of Medical Sciences, Beijing 100050, China.
Wang
B
B
Ma
X
X
D000818:Animals; D002460:Cell Line; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis; D015850:Interleukin-6 / Q000032:analysis; D008099:Liver / Q000166:cytology / Q000187:drug effects*; D008516:Medicine, Chinese Traditional; D016328:NF-kappa B / Q000037:antagonists & inhibitors* / Q000378:metabolism; D051381:Rats
D004365:Drugs, Chinese Herbal; D015850:Interleukin-6; D016328:NF-kappa B; C476499:herbal compound 861; D018799:Intercellular Adhesion Molecule-1