title: Detection of cytokines in human sural nerve biopsies: an immunohistochemical and molecular study.
abstract: In vitro and in vivo models have implicated numerous cytokines as major modulators of inflammation, destruction and repair in the peripheral nervous system (PNS). The in situ production of cytokines in human peripheral nerve disorders is still poorly documented. We studied the expression of interleukin (IL)-1 beta, tumor necrosis factor (TNF)-alpha, IL-6, IL-10, IL-4, IL-3 and nerve growth factor (NGF) in 35 human sural nerve biopsies using immunohistochemistry; additional reverse transcription-polymerase chain reaction and mRNA in situ hybridization were performed for IL-4 and NGF. Expression of IL-1 beta and TNF-alpha was shown in both morphologically normal nerves and various neuropathies, and macrophages appeared as their predominant source. Levels of IL-1 beta and TNF-alpha expression were significantly correlated (P < 0.01) with each other and with expression of NGF. Multiple endoneurial sources were suggested for IL-6 and IL-10 with low immunoreactivity in the vast majority of cases. Conversely, IL-4 and IL-3 expression were found in neuropathies of various etiologies and Schwann cells appeared to be a predominant source of IL-4 in double-labeling immunofluorescence studies. IL-3 immunoreactivity correlated with IL-1 beta, TNF-alpha and IL-6. In this retrospective study, no specific cytokine profile of expression could be assigned to a precise subgroup of neuropathies. This is the first report of IL-4 and IL-3 expression in human neuropathies, and it may be important given the potential role of these cytokines in modulating macrophage activity in the PNS.
authors.affiliation: Department of Pathology, Laboratory of Neuropathology, Tour de Pathologie + 1, University Hospital, CHU University of Liège, 4000 Sart Tilman, Belgium. Manuel.Deprez@ulg.ac.be
authors.forename: M
authors.initials: M
authors.lastname: Deprez
authors.forename: U
authors.initials: U
authors.lastname: Lübke
authors.forename: M
authors.initials: M
authors.lastname: Verlaet
authors.forename: S
authors.initials: S
authors.lastname: Debrus
authors.forename: P
authors.initials: P
authors.lastname: Delvenne
authors.forename: J J
authors.initials: JJ
authors.lastname: Martin
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D001706:Biopsy; D002648:Child; D002675:Child, Preschool; D016207:Cytokines / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D018076:DNA, Complementary / Q000235:genetics; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D020869:Gene Expression Profiling; D006801:Humans; D007124:Immunoenzyme Techniques; D017403:In Situ Hybridization; D007378:Interleukins / Q000032:analysis / Q000235:genetics / Q000276:immunology; D008297:Male; D008856:Microscopy, Fluorescence; D008875:Middle Aged; D009414:Nerve Growth Factors / Q000032:analysis / Q000235:genetics / Q000276:immunology; D009419:Nerve Tissue Proteins / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D010523:Peripheral Nervous System Diseases / Q000378:metabolism* / Q000473:pathology; D013497:Sural Nerve / Q000737:chemistry* / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis / Q000235:genetics / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D016207:Cytokines; D018076:DNA, Complementary; D007378:Interleukins; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D014409:Tumor Necrosis Factor-alpha