Unique subpopulations of CD56+ NK and NK-T peripheral blood lymphocytes identified by chemokine receptor expression repertoire.
CD56, an adhesion molecule closely related to neural cell adhesion molecule, is an immunophenotypic marker for several unique populations of PBLS: Although CD56(+) cells derive from multiple lymphocyte lineages, they share a role in immunosurveillance and antitumor responses. We have studied the chemokine receptor expression patterns and functional migratory responses of three distinct CD56(+) populations from human peripheral blood. NK-T cells were found to differ greatly from NK cells, and CD16(+) NK cells from CD16(-) NK cells. CD16(+) NK cells were the predominant population responding to IL-8 and fractalkine, whereas NK-T cells were the predominant population responding to the CCR5 ligand macrophage-inflammatory protein-1beta. CD16(-) NK cells were the only CD56(+) population that uniformly expressed trafficking molecules necessary for homing into secondary lymphoid organs through high endothelial venule. These findings describe a diverse population of cells that may have trafficking patterns entirely different from each other, and from other lymphocyte types.
Campbell
J J
JJ
Joint Program in Transfusion Medicine, Children's Hospital, 300 Longwood Avenue, Room BD-401, Boston, MA 02115. campbell_ja@tch.harvard.edu
Qin
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Unutmaz
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Soler
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Murphy
K E
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Hodge
M R
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Wu
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Butcher
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D000328:Adult; D019002:CD56 Antigen / Q000096:biosynthesis* / Q000097:blood; D002465:Cell Movement / Q000276:immunology; D018925:Chemokines / Q000097:blood / Q000502:physiology; D002634:Chemotaxis, Leukocyte / Q000276:immunology; D006801:Humans; D016130:Immunophenotyping; D007694:Killer Cells, Natural / Q000378:metabolism* / Q000502:physiology; D008221:Lymphoid Tissue / Q000166:cytology / Q000276:immunology; D009928:Organ Specificity / Q000276:immunology; D019707:Receptors, Chemokine / Q000096:biosynthesis* / Q000097:blood; D017452:Receptors, IgG / Q000096:biosynthesis / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000378:metabolism* / Q000502:physiology
D019002:CD56 Antigen; D018925:Chemokines; D019707:Receptors, Chemokine; D017452:Receptors, IgG