Physical and radiation hybrid mapping of canine chromosome 12, in a region corresponding to human chromosome 6p12-q12.
The positional cloning of the hypocretin receptor 2, the gene for autosomal recessive canine narcolepsy, has led to the development of a physical map spanning a large portion of canine chromosome 12 (CFA12), in a region corresponding to human chromosome 6p12-q13. More than 40 expressed sequence tags (ESTs) were used in homology search experiments, together with chromosome walking, to build both physical and radiation hybrid maps of the CFA12 13-21 region. The resulting map of bacterial artificial chromosome ends, ESTs, and microsatellite markers represents the longest continuous high-density map of the dog genome reported to date. These data further establish the dog as a system for studying disease genes of interest to human populations and highlight feasible approaches for positional cloning of disease genes in organisms where genomic resources are limited.
Li
R
R
Room P-114, Stanford Center for Narcolepsy Research, 1201 Welch Road, Stanford, California 94305-5485, USA.
Faraco
J H
JH
Lin
L
L
Lin
X
X
Hinton
L
L
Rogers
W
W
Lowe
J K
JK
Ostrander
E A
EA
Mignot
E
E
D000818:Animals; D001324:Autoantigens / Q000235:genetics; D002352:Carrier Proteins; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics; D002896:Chromosomes, Human, Pair 6 / Q000235:genetics*; D003094:Collagen / Q000235:genetics; D020451:Contig Mapping; D003598:Cytoskeletal Proteins; D017931:DNA Primers; D004285:Dogs; D000071821:Dystonin; D020224:Expressed Sequence Tags; D008040:Genetic Linkage / Q000235:genetics; D006801:Humans; D018895:Microsatellite Repeats / Q000235:genetics; D009290:Narcolepsy / Q000235:genetics; D009419:Nerve Tissue Proteins; D024041:Non-Fibrillar Collagens; D064446:Orexin Receptors; D011110:Polymorphism, Genetic / Q000235:genetics; D021101:Radiation Hybrid Mapping; D043562:Receptors, G-Protein-Coupled; D018013:Receptors, Neuropeptide / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D016324:Sequence Tagged Sites; D000097805:Collagen Type XVII
D001324:Autoantigens; D002352:Carrier Proteins; D003094:Collagen; D003598:Cytoskeletal Proteins; D017931:DNA Primers; D000071821:Dystonin; D009419:Nerve Tissue Proteins; D024041:Non-Fibrillar Collagens; D064446:Orexin Receptors; D043562:Receptors, G-Protein-Coupled; D018013:Receptors, Neuropeptide; D000097805:Collagen Type XVII; C486268:DST protein, human