Effects of antisense IRAK-2 oligonucleotides on PGI2 release induced by IL-1 and TNF.
OBJECTIVE
To explore the effects of antisense interleukin-1 receptor associated kinase-2 oligonucleotide (IRAK-2 ODN) on the prostacyclin (PGI2) synthesis in human umbilical vein endothelial cells (HUVEC) induced by interleukin-1 (IL-1) and tumor necrosis factor (TNF).


METHODS
The HUVEC were transfected with antisense IRAK-2 ODN and stimulated with IL-1 and TNF. The levels of PGI2 release were analyzed by competitive ELISA.


RESULTS
Pre-transfection with antisense IRAK-2 ODN could remarkably decrease the levels of PGI2 synthesis induced by IL-1 in a time- and concentration-dependent manner, whereas it could not attenuate the one stimulated by TNF.


CONCLUSIONS
The responses of antisense IRAK-2 ODN to IL-1 and TNF-stimulated PGI2 release are different. IRAK-2 plays a key role in the IL-1 signaling events leading to PGI2 release.
Li
Y L
YL
Institute of Pharmaceutics Sciences, The First Military Medical University, Guangzhou 510515, China.
Guo
F K
FK
Wu
S G
SG
D015121:6-Ketoprostaglandin F1 alpha / Q000096:biosynthesis; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D011464:Epoprostenol / Q000096:biosynthesis*; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology; D053592:Interleukin-1 Receptor-Associated Kinases; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D011494:Protein Kinases / Q000494:pharmacology*; D017472:Receptors, Interleukin-1; D015398:Signal Transduction; D014162:Transfection; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D007375:Interleukin-1; D016376:Oligonucleotides, Antisense; D017472:Receptors, Interleukin-1; D014409:Tumor Necrosis Factor-alpha; D015121:6-Ketoprostaglandin F1 alpha; D011464:Epoprostenol; D011494:Protein Kinases; D053592:Interleukin-1 Receptor-Associated Kinases