title: [Depolymerization of chitosan by chinolytic complex from Bacillus sp. 739].
abstract: Low-molecular-weight (3-6 kDa) water-soluble chitosan was obtained by enzymatic depolymerization. Hydrolysis of crab chitosan was induced by O-glycoside hydrolase (EC 3.2.1), an extracellular chitinolytic complex from Bacillus sp. 739. The optimum conditions for hydrolysis were found (sodium-acetate buffer, pH 5.2; 55 degrees C; an enzyme/substrate ratio 4 U/g chitosan; 1 h).
authors.affiliation: Bioinzheneriya Center, Russian Academy of Sciences, Moscow, 117312 Russia.
authors.forename: A V
authors.initials: AV
authors.lastname: Il'ina
authors.forename: V P
authors.initials: VP
authors.lastname: Varlamov
authors.forename: A I
authors.initials: AI
authors.lastname: Melent'ev
authors.forename: G E
authors.initials: GE
authors.lastname: Aktuganov
mesh_terms: D000118:Acetylglucosaminidase / Q000378:metabolism*; D001407:Bacillus / Q000201:enzymology*; D002686:Chitin / Q000031:analogs & derivatives / Q000378:metabolism*; D002688:Chitinases / Q000378:metabolism*; D048271:Chitosan; D006868:Hydrolysis
chemical_list: D002686:Chitin; D048271:Chitosan; D002688:Chitinases; D000118:Acetylglucosaminidase