[The construction and expression of 6B11scFv anti-idiotypic antibody and human granulocyte-macrophage-colony stimulating factor in Escherichia coli].
OBJECTIVE
To enhance the antigenicity of an anti-idiotypic single chain antibody 6B11 mimicking ovarian cancer antigen.


METHODS
Using DNA recombinant technique, a recombinant fusion protein expression vector was constructed. This vector linked 6B11scFv cDNA and human GM-CSF with a linker. Protein productions were induced with the temperature change in E. Coli and analyzed with western blot.


RESULTS
The expressed fusion protein can be reacted with both anti-human antibody and COC166-9(AB1 of 6B11).


CONCLUSIONS
The recombinant fusion protein keeps the activities of both components and may be used as tumor vaccine for ovarian cancer.
Yang
F
F
Gynecologic Oncology Center, People's Hospital, Bejing Medical University, Beijing 100034.
Qian
H
H
Huang
H
H
D000888:Antibodies, Anti-Idiotypic / Q000235:genetics / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D000912:Antibodies, Neoplasm / Q000276:immunology*; D003536:Cystadenocarcinoma / Q000276:immunology*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000235:genetics / Q000276:immunology*; D006801:Humans; D010051:Ovarian Neoplasms / Q000276:immunology*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm; D011993:Recombinant Fusion Proteins; D016178:Granulocyte-Macrophage Colony-Stimulating Factor