RNA extraction from ethanol-fixed leukocytes trapped on polyethylene terephthalate filter for RT-PCR analysis.
Yokota
M
M
Osaka City University Postgraduate Medical School, Osaka, Japan.
Hino
M
M
Tatsumi
N
N
Nishioka
T
T
Tsuda
I
I
D000328:Adult; D000925:Anticoagulants; D004492:Edetic Acid; D000431:Ethanol; D005404:Fixatives; D016044:Fusion Proteins, bcr-abl / Q000235:genetics; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics; D006801:Humans; D016898:Interferon-alpha / Q000627:therapeutic use; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics; D007962:Leukocytes / Q000737:chemistry*; D011093:Polyethylene Terephthalates; D012313:RNA / Q000302:isolation & purification*; D012333:RNA, Messenger / Q000097:blood; D012194:RNA-Directed DNA Polymerase; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013053:Spectrophotometry
D000925:Anticoagulants; D005404:Fixatives; D016898:Interferon-alpha; D011093:Polyethylene Terephthalates; D012333:RNA, Messenger; D000431:Ethanol; D012313:RNA; D004492:Edetic Acid; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D016044:Fusion Proteins, bcr-abl; D012194:RNA-Directed DNA Polymerase