title: The molecular basis of allergenicity: comparative analysis of the three dimensional structures of diverse allergens reveals a common structural motif.
abstract: BACKGROUND
  Although a large number of allergens have been characterised, the structural, functional, and biochemical features that these molecules have in common, and that could explain their ability to elicit powerful IgE antibody responses, are still uncertain. Recently, there has been considerable interest in the role of the cysteine protease activity of the house dust mite allergen Der p 1 in biasing the immune response in favour of IgE production.
  
  
  OBJECTIVE
  To search for remote homologues of Der p 1 with sequences similar to the 30 conserved amino acids surrounding the catalytic cysteine residue (Cys34).
  
  
  METHODS
  Potential homologues were analysed by examining their three dimensional structures and multiple sequence alignments using the programs PROPSEARCH, ClustalW, GeneDoc, and Swiss Pdb Viewer.
  
  
  RESULTS
  Diverse allergens (for example, the plant cysteine protease papain, the transport protein lipocalin Mus m 1, and the ragweed allergen Amb a 5) have a similar structural motif; namely, a groove resembling the substrate binding groove of Der p 1. The groove is located inside an alpha-beta motif, between an alpha helix on one side and an antiparallel beta sheet on the other side. A similar common motif (a cysteine stabilised alpha-beta fold) can also be found in some toxins and defensins.
  
  
  CONCLUSIONS
  Allergens of diverse sources have a common structural motif, namely a groove located inside an alpha-beta motif, which could potentially serve as a ligand binding site.
authors.affiliation: Division of Molecular and Clinical Immunology, Faculty of Medicine and Health Sciences, Queen's Medical Centre, University of Nottingham, Nottingham NG7 2UH, UK.
authors.forename: R
authors.initials: R
authors.lastname: Furmonaviciene
authors.forename: F
authors.initials: F
authors.lastname: Shakib
mesh_terms: D000485:Allergens / Q000737:chemistry*; D000818:Animals; D039741:Antigens, Dermatophagoides; D001665:Binding Sites; D002352:Carrier Proteins / Q000737:chemistry; D003545:Cysteine / Q000737:chemistry*; D003546:Cysteine Endopeptidases / Q000737:chemistry; D006023:Glycoproteins / Q000737:chemistry*; D054835:Lipocalin 1; D010947:Plants, Toxic; D012674:Senecio / Q000737:chemistry; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein; D012984:Software
chemical_list: D000485:Allergens; D039741:Antigens, Dermatophagoides; D002352:Carrier Proteins; D006023:Glycoproteins; D054835:Lipocalin 1; D003546:Cysteine Endopeptidases; D003545:Cysteine