A glia-derived acetylcholine-binding protein that modulates synaptic transmission.
There is accumulating evidence that glial cells actively modulate neuronal synaptic transmission. We identified a glia-derived soluble acetylcholine-binding protein (AChBP), which is a naturally occurring analogue of the ligand-binding domains of the nicotinic acetylcholine receptors (nAChRs). Like the nAChRs, it assembles into a homopentamer with ligand-binding characteristics that are typical for a nicotinic receptor; unlike the nAChRs, however, it lacks the domains to form a transmembrane ion channel. Presynaptic release of acetylcholine induces the secretion of AChBP through the glial secretory pathway. We describe a molecular and cellular mechanism by which glial cells release AChBP in the synaptic cleft, and propose a model for how they actively regulate cholinergic transmission between neurons in the central nervous system.
Smit
A B
AB
Department of Molecular and Cellular Neurobiology, Research Institute Neurosciences Vrije Universiteit, Faculty of Biology, De Boelelaan 1087, 1081 HV Amsterdam, The Netherlands. absmit@bio.vu.nl
Syed
N I
NI
Schaap
D
D
van Minnen
J
J
Klumperman
J
J
Kits
K S
KS
Lodder
H
H
van der Schors
R C
RC
van Elk
R
R
Sorgedrager
B
B
Brejc
K
K
Sixma
T K
TK
Geraerts
W P
WP
D000109:Acetylcholine / Q000378:metabolism* / Q000494:pharmacology; D000595:Amino Acid Sequence; D000818:Animals; D002038:Bungarotoxins / Q000378:metabolism / Q000494:pharmacology; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D002478:Cells, Cultured; D002490:Central Nervous System / Q000166:cytology / Q000378:metabolism; D018920:Coculture Techniques; D020128:Inhibitory Concentration 50; D008024:Ligands; D008195:Lymnaea / Q000737:chemistry / Q000235:genetics / Q000502:physiology; D008959:Models, Neurological; D008969:Molecular Sequence Data; D009457:Neuroglia / Q000737:chemistry / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D011485:Protein Binding; D021382:Protein Sorting Signals; D020836:Protein Structure, Quaternary; D017434:Protein Structure, Tertiary; D021381:Protein Transport; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D011978:Receptors, Nicotinic / Q000737:chemistry / Q000378:metabolism; D016415:Sequence Alignment; D012701:Serotonin / Q000378:metabolism / Q000494:pharmacology; D009435:Synaptic Transmission / Q000187:drug effects
C428944:AChBP protein, Lymnaea; D002038:Bungarotoxins; D002352:Carrier Proteins; D008024:Ligands; D021382:Protein Sorting Signals; D012333:RNA, Messenger; D011978:Receptors, Nicotinic; D012701:Serotonin; D000109:Acetylcholine