title: Ischaemic preconditioning in the rat brain: a longitudinal magnetic resonance imaging (MRI) study.
abstract: Ischaemic preconditioning in rats was studied using MRI. Ischaemic preconditioning was induced, using an intraluminal filament method, by 30 min middle cerebral artery occlusion (MCAO), and imaged 24 h later. The secondary insult of 100 min MCAO was induced 3 days following preconditioning and imaged 24 and 72 h later. Twenty-four hours following ischaemic preconditioning most rats showed small sub-cortical hyperintense regions not seen in sham-preconditioned rats. Twenty-four hours and 72 h following the secondary insult preconditioned animals showed significantly smaller lesions (24 h = 112 +/- 31 mm(3), mean +/- standard error; 72 h = 80 +/- 35 mm(3)), which were confined to the striatum, than controls (24 h = 234 +/- 32 mm(3), p = 0.026; 72 h = 275 +/- 37 mm(3), p = 0.003). In addition during lesion maturation from 24 to 72 h post-secondary MCAO, preconditioned rats displayed an average reduction in lesion size as measured by MRI whereas sham-preconditioned rats displayed increases in lesion size; this is the first report of such differential lesion volume evolution in cerebral ischaemic preconditioning.
authors.affiliation: Safety Assessment, GlaxoSmithKline, The Frythe, Welwyn, Herts AL6 9AR, UK.
authors.forename: P G
authors.initials: PG
authors.lastname: Mullins
authors.forename: D G
authors.initials: DG
authors.lastname: Reid
authors.forename: P D
authors.initials: PD
authors.lastname: Hockings
authors.forename: S J
authors.initials: SJ
authors.lastname: Hadingham
authors.forename: C A
authors.initials: CA
authors.lastname: Campbell
authors.forename: J B
authors.initials: JB
authors.lastname: Chalk
authors.forename: D M
authors.initials: DM
authors.lastname: Doddrell
mesh_terms: D000818:Animals; D002546:Ischemic Attack, Transient / Q000473:pathology*; D019194:Ischemic Preconditioning; D008137:Longitudinal Studies; D008279:Magnetic Resonance Imaging; D008297:Male; D020768:Middle Cerebral Artery; D051381:Rats; D017207:Rats, Sprague-Dawley