title: Antigen-induced translocation of PKC-theta to membrane rafts is required for T cell activation.
abstract: Protein kinase C-theta (PKC-theta) is essential for mature T cell activation; however, the mechanism by which it is recruited to the TCR signaling machinery is unknown. Here we show that T cell stimulation by antibodies or peptide-major histocompatibility complex (MHC) induces translocation of PKC-theta to membrane lipid rafts, which localize to the immunological synapse. Raft translocation was mediated by the PKC-theta regulatory domain and required Lck but not ZAP-70. In addition, PKC-theta was associated with Lck in the rafts. An isolated PKC-straight theta catalytic fragment did not partition into rafts or activate the transcription factor NF-kappa B, although addition of a Lck-derived raft-localization sequence restored these functions. Thus, physiological T cell activation translocates PKC-theta to rafts, which localize to the T cell synapse; this PKC-theta translocation is important for its function.
authors.affiliation: Division of Cell Biology, La Jolla Institute for Allergy and Immunology, 10355 Science Center Drive, San Diego, CA 92121, USA.
authors.forename: K
authors.initials: K
authors.lastname: Bi
authors.forename: Y
authors.initials: Y
authors.lastname: Tanaka
authors.forename: N
authors.initials: N
authors.lastname: Coudronniere
authors.forename: K
authors.initials: K
authors.lastname: Sugie
authors.forename: S
authors.initials: S
authors.lastname: Hong
authors.forename: M J
authors.initials: MJ
authors.lastname: van Stipdonk
authors.forename: A
authors.initials: A
authors.lastname: Altman
mesh_terms: D000938:Antigen-Presenting Cells / Q000276:immunology; D000941:Antigens / Q000378:metabolism*; D001693:Biological Transport, Active; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism*; D019169:Jurkat Cells; D008213:Lymphocyte Activation; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck) / Q000378:metabolism; D008563:Membrane Lipids / Q000276:immunology; D010766:Phosphorylation; D011493:Protein Kinase C / Q000378:metabolism*; D000074121:Protein Kinase C-theta; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D013601:T-Lymphocytes / Q000201:enzymology* / Q000276:immunology*; D051746:ZAP-70 Protein-Tyrosine Kinase
chemical_list: D000941:Antigens; D007527:Isoenzymes; D008563:Membrane Lipids; D011505:Protein-Tyrosine Kinases; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck); D051746:ZAP-70 Protein-Tyrosine Kinase; C497261:ZAP70 protein, human; C076233:PRKCQ protein, human; D011493:Protein Kinase C; D000074121:Protein Kinase C-theta