Prevalence of iron transport gene on pathogenicity-associated island of uropathogenic Escherichia coli in E. coli O157:H7 containing Shiga toxin gene.
Uropathogenc Escherichia coli (UPEC) CFT073 has a pathogenicity-associated island (PAI(CFT073)), which causes pyelonephritis and cystitis. Using PCR method, we found the prrA gene of PAI(CFT073) in E. coli O157:H7 EDL933. Further detailed PCR screening of 38 open reading frames, the right and left junction sequences of PAI(CFT073), revealed that it is the prrA-modD-yc73-fepC gene cluster but not the PAI(CFT073) present in E. coli O157:H7 EDL933. A rapid preliminary analysis suggested that the prrA-modD-yc73-fepC gene cluster of the PAI(CFT073), is present in 43 strains of E. coli O157:H7 containing Shiga toxin (Stx) gene but absent in 19 strains of E. coli O157:H7 without Stx gene. A strict co-occurrence of the prrA-modD-yc73-fepC gene cluster and Stx genes was observed, regardless of their origin. The prrA-modD-yc73-fepC gene cluster encode proteins probably involved in iron uptake system, which strongly suggests the importance of iron metabolism in the Stx-mediated virulence. In addition, the prrA-modD-yc73-fepC gene cluster may be used as a diagnostic marker to distinguish E. coli O157:H7 strains containing Stx gene from that without Stx gene, and possibly to quickly detect other pathogenic gram-negative bacteria containing the Stx gene.
Ye
C
C
Priority Laboratory of Medical Molecular Bacteriology of the Ministry of Health, Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Changping, Beijing 102206, People's Republic of China.
Xu
J
J
D000818:Animals; D003556:Cystitis / Q000382:microbiology; D019453:Escherichia coli O157 / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity*; D005798:Genes, Bacterial; D006801:Humans; D007501:Iron / Q000378:metabolism*; D005810:Multigene Family; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction / Q000379:methods; D011704:Pyelonephritis / Q000382:microbiology; D022603:Shiga Toxins / Q000235:genetics* / Q000378:metabolism; D014774:Virulence / Q000235:genetics
D022603:Shiga Toxins; D007501:Iron