Disulfide structure of alfimeprase: a recombinant analog of fibrolase.
The disulfide structure of alfimeprase, a recombinant analog of fibrolase, was experimentally determined by a combination of peptide mapping, Edman degradation, and mass spectrometry. The three disulfide bonds were determined to be Cys-116/196, Cys-156 /180, and Cys-158/163 with the residue number system of alfimeprase.
Jones
G
G
Analytical Research & Development Department, Amgen Inc., Thousand Oaks, California 91320, USA.
Ronk
M
M
Mori
F
F
Zhang
Z
Z
D000595:Amino Acid Sequence; D004220:Disulfides / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry; D013058:Mass Spectrometry; D008666:Metalloendopeptidases / Q000737:chemistry*; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry; D010843:Pichia / Q000201:enzymology; D011994:Recombinant Proteins / Q000737:chemistry; D020539:Sequence Analysis, Protein
D004220:Disulfides; D010455:Peptides; D011994:Recombinant Proteins; D008666:Metalloendopeptidases; C436755:alfimeprase; C058789:fibrolase