Visualizing chromosome dynamics with GFP.
By allowing easy labeling of chromosomal and nuclear proteins and the tagging of specific chromosomal regions, the use of green-fluorescent protein (GFP) has provided new and special opportunities for directly observing chromosome dynamics in vivo. Here, we review recent applications of this methodology, focusing particularly on examples where new biology has been learned, or at least sighted. In particular, we focus on active bacterial chromosome segregation, yeast mitosis and centromere dynamics, and large-scale chromatin structure and dynamics within eukaryotic interphase nuclei.
Belmont
A S
AS
Dept of Cell and Structural Biology, University of Illinois, Urbana-Champaign, B107 CLSL, 601 South Goodwin Ave., Urbana, IL 61801, USA. asbel@uiuc.edu
D000818:Animals; D002503:Centromere / Q000378:metabolism; D002842:Chromatids / Q000378:metabolism; D020090:Chromosome Segregation; D002876:Chromosomes, Bacterial / Q000378:metabolism*; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins; D007202:Indicators and Reagents; D008164:Luminescent Proteins; D008938:Mitosis
D007202:Indicators and Reagents; D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins