[CYP11B2 expression in rat liver and the efficacy of antisterone on liver fibrosis].
OBJECTIVE
To identify aldosterone synthase gene-CYP11B2 mRNA expression in rat livers and evaluate the efficacy of antisterone.


METHODS
One hundred and twenty male Wistar rats were divided into 3 groups: model group (subcutaneous injection of CCl(4), 0.25 ml/100 mg, 3 times/week), antisterone group (besides same dosage of CCl(4) given, gastropufusion of antisterone of 20mg x kg(-1) x d(-1)), and control group (subcutaneous injection of olive oil). The area of collagen was examined by image analyzing system. The expression of aldosterone synthase gene-CYP11B2 mRNA was detected by RT-PCR and in situ hybridization.


RESULTS
The expression of CYP11B2 mRNA, which located in the endoplasm of HSC, was upregulated when fibrogenesis occurred. The grade of fibrosis and the area of collagen in antisterone group were less than those in model group before 6th week (P<0.05). After the 6th week however, there was no significant difference between the two groups (P>0.05).


CONCLUSIONS
The expression of CYP11B2 mRNA is upregulated in fibrotic liver. Antisterone can partly have a fibrogenesis-inhibiting effect on the early stage of CCl(4)-induced hepatic fibrosis.
Li
X
X
Institute for Digestive Diseases of PLA, Nanfang Hospital of First Military Medical University, Guangzhou 510515, China.
Meng
Y
Y
Yang
X
X
Wu
P
P
Zhang
H
H
Li
S
S
Lai
W
W
D000818:Animals; D019405:Cytochrome P-450 CYP11B2 / Q000235:genetics*; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D015971:Gene Expression Regulation, Enzymologic; D008099:Liver / Q000166:cytology / Q000201:enzymology*; D008106:Liver Cirrhosis, Experimental / Q000188:drug therapy* / Q000201:enzymology; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D004365:Drugs, Chinese Herbal; D019405:Cytochrome P-450 CYP11B2