Immunohistochemical study on proliferative activity of experimental cholesteatoma.
In this study, we induced canal ligation cholesteatoma using Mongolian gerbils and investigated the hyperproliferative nature of canal ligation cholesteatoma by using an immunohistochemical technique with proliferation markers (cytokeratin 13/16, PCNA, EGFR, thrombomodulin). Canal ligation cholesteatoma using Mongolian gerbils had a hyperproliferative character in the suprabasal cell layer similar to human cholesteatomas. This result will provide a good morphological basis for future cholesteatoma animal research concerning the pathogenesis of cholesteatoma.
Park
K
K
Department of Otolaryngology, Ajou University School of Medicine, 5 Wonchon-Dong, Paldal-Gu, Suwon 442-721, Korea. parkkh@madang.ajou.ac.kr
Park
H J
HJ
Chun
Y M
YM
D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D002455:Cell Division / Q000502:physiology*; D018424:Cholesteatoma, Middle Ear / Q000473:pathology*; D004424:Ear Canal / Q000473:pathology; D004848:Epithelium / Q000473:pathology; D066246:ErbB Receptors / Q000032:analysis; D006168:Guinea Pigs; D007124:Immunoenzyme Techniques; D007633:Keratins / Q000032:analysis; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D018180:Thrombomodulin / Q000032:analysis
D018809:Proliferating Cell Nuclear Antigen; D018180:Thrombomodulin; D007633:Keratins; D066246:ErbB Receptors