Perinuclear mRNA localisation by vimentin 3'-untranslated region requires a 100 nucleotide sequence and intermediate filaments.
The role of the vimentin 3'-untranslated region (3'-UTR) in mRNA localisation was studied in cells transfected with a reporter sequence linked to subregions of the 3'-UTR. In situ hybridisation showed that nucleotides 37-137, including a previously identified protein-binding domain, were sufficient to localise transcripts to perinuclear cytoplasm. Transfection of two SW13 cell lines that do and do not express vimentin showed that perinuclear localisation due to either the vimentin or c-myc 3'-UTR requires intermediate filaments. The data suggest that both a specific protein-binding region of the vimentin 3'-UTR and intermediate filaments themselves are required to determine the site of vimentin synthesis.
Bermano
G
G
Intrecellular Targeting Group, Rowett Research Institute Aberdeen, UK.
Shepherd
R K
RK
Zehner
Z E
ZE
Hesketh
J E
JE
D020413:3' Untranslated Regions / Q000032:analysis / Q000378:metabolism*; D000818:Animals; D016466:CHO Cells; D002467:Cell Nucleus / Q000378:metabolism*; D006224:Cricetinae; D003593:Cytoplasm / Q000378:metabolism; D017930:Genes, Reporter; D006801:Humans; D017403:In Situ Hybridization; D007382:Intermediate Filaments / Q000378:metabolism*; D011485:Protein Binding / Q000235:genetics; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism*; D012045:Regulatory Sequences, Nucleic Acid; D014162:Transfection; D014407:Tumor Cells, Cultured; D014746:Vimentin / Q000235:genetics* / Q000378:metabolism
D020413:3' Untranslated Regions; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger; D014746:Vimentin