The effect of thyroid stimulating hormone on the proliferation and viability of HEP-2 laryngeal cells in culture.
The objective of this experiment was to study the effect of TSH on Hep-2 cells. A total of sixteen tubes (5 x 10(4) cells per tube) were divided into four equal groups (media alone (control), serum containing 0 TSH, 10.3 microliters/ml TSH, and 49 microliters/ml TSH). The supernatants and cells were collected at 24, 48, and 72 hours after incubation. The result show that TSH caused an increase in cell number after 24 hours in comparison to control media alone. Analysis of supernatants for cellular damage showed an increased pattern in MDA levels in serum exposed cells at 24, 48, and 72 hours. In contrast, MDA levels in TSH treated cells were similar at 24, 48, and 72 hours. The levels obtained at 48 and 72 hours were statistically (P < 0.05) lower than those obtained for control and serum treated or 0 TSH group. This observation suggests that TSH could provide a protective measure against membrane lipid peroxidation. Morphological evaluation of the cells at 24, 48, and 72 hours, suggests that TSH exposure did induce noticeable cellular injury and most adaptive responses observed were shape changes (round up), cellular detachment, and hyperchromatic nuclei (decrease in cell number).
Sodachanh
D
D
University of Mississippi Medical Center, Jackson, MS 39216, USA.
Benghuzzi
H
H
Tucci
M
M
Cason
Z
Z
D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D007830:Larynx / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D011506:Proteins / Q000378:metabolism; D013972:Thyrotropin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D011506:Proteins; D008315:Malondialdehyde; D013972:Thyrotropin