title: Oxacillinase-mediated resistance to cefepime and susceptibility to ceftazidime in Pseudomonas aeruginosa.
abstract: Pseudomonas aeruginosa clinical isolate SOF-1 was resistant to cefepime and susceptible to ceftazidime. This resistance phenotype was explained by the expression of OXA-31, which shared 98% amino acid identity with a class D beta-lactamase, OXA-1. The oxa-31 gene was located on a ca. 300-kb nonconjugative plasmid and on a class 1 integron. No additional efflux mechanism for cefepime was detected in P. aeruginosa SOF-1. Resistance to cefepime and susceptibility to ceftazidime in P. aeruginosa were conferred by OXA-1 as well.
authors.affiliation: Service de Bactériologie-Virologie, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris-Sud, 94275 Le Kremlin-Bicêtre, France.
authors.forename: D
authors.initials: D
authors.lastname: Aubert
authors.forename: L
authors.initials: L
authors.lastname: Poirel
authors.forename: J
authors.initials: J
authors.lastname: Chevalier
authors.forename: S
authors.initials: S
authors.lastname: Leotard
authors.forename: J M
authors.initials: JM
authors.lastname: Pages
authors.forename: P
authors.initials: P
authors.lastname: Nordmann
mesh_terms: D000077723:Cefepime; D002442:Ceftazidime / Q000494:pharmacology*; D002511:Cephalosporins / Q000494:pharmacology*; D004352:Drug Resistance, Microbial; D006801:Humans; D007223:Infant; D008826:Microbial Sensitivity Tests; D010641:Phenotype; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D002511:Cephalosporins; D000077723:Cefepime; D002442:Ceftazidime; C039404:beta-lactamase OXA-2; D001618:beta-Lactamases