S-phase dependent cytogenetic toxicity of 5-fluorouracil in bone marrow cells of mice.
The in vivo cytogenetic toxicity of three different doses (5,10 and 15 mg/kg) of 5-fluorouracil (5-FU) (in Fluracil) was assessed in bone marrow cells of mice. At 24 hrs post-treatment the induced chromosomal aberrations, mostly chromatid breaks and fragments, by all the three different concentrations of 5-FU were found statistically significant. However, mitotic index study at 24 hrs post-treatment indicated it as nonmitotoxic. At 30 hrs posttreatment, all the three doses of 5-FU induced a statistically significant number of micronuclei per thousand polychromatic erythrocytes. This indicated 5-FU as nonmitotoxic but highly clastogenic in bone marrow cells of mice.
Choudhury
R C
RC
Dept. of Zoology, Berhampur University, Orissa, India. rameshchoudary@hotmail.com
Misra
S
S
Jagdale
M B
MB
Palo
A K
AK
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects* / Q000473:pathology; D002460:Cell Line; D002842:Chromatids / Q000187:drug effects; D002869:Chromosome Aberrations; D006224:Cricetinae; D003520:Cyclophosphamide / Q000494:pharmacology / Q000633:toxicity; D004331:Drosophila melanogaster / Q000235:genetics; D005260:Female; D005472:Fluorouracil / Q000494:pharmacology / Q000633:toxicity*; D006801:Humans; D007700:Kinetics; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008815:Mice, Inbred Strains; D015162:Micronucleus Tests; D008940:Mitotic Index; D009152:Mutagenicity Tests; D009153:Mutagens / Q000494:pharmacology*; D016196:S Phase / Q000187:drug effects*; D012486:Salmonella typhimurium / Q000187:drug effects; D013997:Time Factors
D009153:Mutagens; D003520:Cyclophosphamide; D005472:Fluorouracil