[Construction of a pufferfish bacterial artificial chromosome library].
OBJECTIVE
Study of human genome by taking advantage of investigating pufferfish's (Fugu rubripes), compact model vertebrate genome.


METHODS
Construct a pufferfish bacterial artificial chromosome (BAC) library using Japanese pufferfish (Fugu rubripes) sperm DNA and pBelo BAC11 vector.


RESULTS
The library consists of 23,040 clones, which have been arrayed in 240,96-well microtiter plates. 100 BAC clones have been selected at random and analyzed for estimation of the average insert size, which turns out to be 100 kb indicating its nearly 6-fold coverage of pufferfish haploid genome. Restrict analysis of 10 BAC clones show that these inserts are stable even propagated for at least 100 cell generations.


CONCLUSIONS
The library accords with the demands of a BAC library.
Liu
S
S
Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005.
Liu
Y
Y
Zhang
X
X
Zhou
W
W
Yang
L
L
D000818:Animals; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics*; D005400:Fishes, Poisonous / Q000235:genetics*; D015698:Genomic Library