title: Cell lysis in Escherichia coli cultures stimulates growth and biosynthesis of recombinant proteins in surviving cells.
abstract: Cell growth and production of recombinant proteins in stationary phase cultures of Escherichia coli recover concomitantly with spontaneous lysis of a fraction of the ageing cell population. Further exploration of this event has indicated that sonic cell disruption stimulates both cell growth and synthesis of plasmid-encoded recombinant proteins, even in exponentially growing cultures. These observations indicate an efficient cell utilisation of released intracellular material and also that this capability is not restricted to extreme nutrient-starving conditions. In addition, the efficient re-conversion of waste cell material can be viewed as a potential strategy for an extreme exploitation of carbon sources and cell metabolites in production processes of both recombinant and non-recombinant microbial products.
authors.affiliation: Institut de Biologia Fonamental and Departament de Genètica i Microbiologia, Universitat Autònoma de Barcelona, Bellaterra, Spain.
authors.forename: J L
authors.initials: JL
authors.lastname: Corchero
authors.forename: R
authors.initials: R
authors.lastname: Cubarsí
authors.forename: P
authors.initials: P
authors.lastname: Vila
authors.forename: A
authors.initials: A
authors.lastname: Arís
authors.forename: A
authors.initials: A
authors.lastname: Villaverde
mesh_terms: D004926:Escherichia coli / Q000254:growth & development / Q000378:metabolism*; D011994:Recombinant Proteins / Q000032:analysis / Q000096:biosynthesis*; D013010:Sonication; D013696:Temperature; D001616:beta-Galactosidase / Q000032:analysis / Q000096:biosynthesis
chemical_list: D011994:Recombinant Proteins; D001616:beta-Galactosidase