[Correlation between electrokinetic parameters of isolated hepatocyte nuclei and their functional activity in rats].
The results of investigations of the electrokinetic properties of cell nuclei in various functional states were summarized. A positive correlation between the value of the electrostatic charge on the nuclear membrane surface and the functional state of the genetic apparatus upon activation was established. It was shown that the value of the electrokinetic potential reflects differences in the mechanisms of action of biologically active substances, and that this may be related to the peculiarity of their action on both membrane surface and the cell genome. It is concluded that the electrokinetic properties of the nuclear membrane reflect changes occurring in the genome of the eukaryotic cell nucleus, and from the value of surface charge density, the extent of involvement of the genome under various influences can be determined.
Vardevanian
P O
PO
Yerevan State University, ul. A. Manukyana 1, Yerevan, 375025 Armenia.
Gonian
S A
SA
Tiratsuian
S G
SG
D000596:Amino Acids / Q000494:pharmacology; D000818:Animals; D002467:Cell Nucleus / Q000201:enzymology / Q000235:genetics / Q000502:physiology*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D003609:Dactinomycin / Q000494:pharmacology; D004586:Electrophoresis; D016678:Genome; D022781:Hepatocytes / Q000648:ultrastructure*; D006854:Hydrocortisone / Q000494:pharmacology; D066298:In Vitro Techniques; D007425:Intracellular Membranes / Q000502:physiology; D008564:Membrane Potentials; D051381:Rats; D014158:Transcription, Genetic
D000596:Amino Acids; D003609:Dactinomycin; D012321:DNA-Directed RNA Polymerases; D006854:Hydrocortisone