title: Mast cells and their secretory granules are angiogenic in the chick embryo chorioallantoic membrane.
abstract: BACKGROUND
  Many data suggest that the density of mast cells is highly correlated with the extent of both normal and pathological angiogenesis.
  
  
  OBJECTIVE
  In this study we have compared in an in vivo assay, the chick embryo chorioallantoic membrane, the angiogenic potential of mast cell suspensions isolated from rats, degranulated mast cells and their secretory granules.
  
  
  METHODS
  Gelatin sponges adsorbed with cell suspensions of rat mast cells, degranulated mast cells and their secretory granules were implanted on the top of the chorioallantoic membrane at day 8 of incubation. At day 12 the angiogenic response was evaluated macroscopically, microscopically and by a morphometric method of 'point counting'.
  
  
  RESULTS
  Isolated mast cells and their secretory granules, but not degranulated mast cells, induced an angiogenic response in the chorioallantoic membrane. The addition of antifibroblast growth factor-2 or antivascular endothelial growth factor antibodies reduced the angiogenic response of both mast cells and their secretory granules by 50% and 30%, respectively.
  
  
  CONCLUSIONS
  These data support the evidence that the angiogenic properties of mast cells depend on the angiogenic molecules contained in their secretory granules and indicate that fibroblast growth factor-2 and vascular endothelial growth factor are the angiogenic cytokines primarily and perhaps synergistically responsible for this vasoproliferative activity.
authors.affiliation: Department of Human Anatomy and Histology, University of Bari Medical School, Bari, Italy. ribatti@anatomia.uniba.it
authors.forename: D
authors.initials: D
authors.lastname: Ribatti
authors.forename: E
authors.initials: E
authors.lastname: Crivellato
authors.forename: L
authors.initials: L
authors.lastname: Candussio
authors.forename: B
authors.initials: B
authors.lastname: Nico
authors.forename: A
authors.initials: A
authors.lastname: Vacca
authors.forename: L
authors.initials: L
authors.lastname: Roncali
authors.forename: F
authors.initials: F
authors.lastname: Dammacco
mesh_terms: D000482:Allantois / Q000502:physiology*; D000818:Animals; D002642:Chick Embryo; D002823:Chorion / Q000502:physiology*; D016228:Endothelial Growth Factors / Q000276:immunology / Q000494:pharmacology; D016222:Fibroblast Growth Factor 2 / Q000276:immunology / Q000494:pharmacology; D008222:Lymphokines / Q000276:immunology / Q000494:pharmacology; D008407:Mast Cells / Q000502:physiology*; D008853:Microscopy; D023421:Models, Animal; D018919:Neovascularization, Physiologic / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D022142:Secretory Vesicles / Q000502:physiology*; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
chemical_list: D016228:Endothelial Growth Factors; D008222:Lymphokines; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; D016222:Fibroblast Growth Factor 2