title: The three-dimensional architecture of retinal blood vessels in KK mice, with special reference to the smooth muscle cells and pericytes.
abstract: In the present study, the three-dimensional architecture of retinal vasculature was studied in KK mice, by combined use of resin injection, chemical treatment and scanning electron microscopy (SEM). In particular, Mercox/methylmethacrylate resin-injected eye tissues were subjected in sequence to NaClO immersion, ultrasonication and HCl treatment. The present technological innovation made possible SEM visualization of deeper retinal vasculature. In KK mice, the tunica media of stem arterioles and of first and second order branches consisted of a single layer of spindle-shaped smooth muscle cells provided with spine-like cytoplasmic processes. In addition, there occurred small triangular smooth muscle cells provided with slender cytoplasmic processes. The processes, giving off tiny secondary processes, overlapped with each other, thus forming assemblages around branching sites. Such a structure was particularly prominent for those branching sites where parent arterioles gave rise to their branches in a side arm-like pattern. The third (and occasionally fourth) order branches were surrounded by atypical smooth muscle cells, with considerable dimension of endothelial surface remaining uncovered. Capillary pericytes consisted of fusiform cell bodies and slender cytoplasmic processes. Smooth muscle cells of retinal venules differed from those of arterioles. They were stellate in shape, exhibiting several cytoplasmic processes.
authors.affiliation: Department of Ophthalmology, Oita Medical University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Ikebe
authors.forename: T
authors.initials: T
authors.lastname: Shimada
authors.forename: K
authors.initials: K
authors.lastname: Ina
authors.forename: H
authors.initials: H
authors.lastname: Kitamura
authors.forename: K
authors.initials: K
authors.lastname: Nakatsuka
mesh_terms: D000818:Animals; D001160:Arterioles / Q000648:ultrastructure; D002196:Capillaries / Q000648:ultrastructure; D006851:Hydrochloric Acid; D008297:Male; D020366:Methylmethacrylate; D051379:Mice; D008855:Microscopy, Electron, Scanning; D009130:Muscle, Smooth / Q000166:cytology / Q000648:ultrastructure*; D020286:Pericytes / Q000648:ultrastructure*; D012171:Retinal Vessels / Q000648:ultrastructure*; D012973:Sodium Hypochlorite; D013010:Sonication
chemical_list: D020366:Methylmethacrylate; D012973:Sodium Hypochlorite; D006851:Hydrochloric Acid