title: [Sequential isolation of superoxide dismutase and ajmaline from a tissue culture of Rauwolfia serpentina Benth].
abstract: Two biologically active compounds, the enzyme superoxide dismutase (EC 1.15.1.1) and the antiarrhythmic indole alkaloid ajmaline, were isolated from a callus culture of Rauwolfia serpentina Benth. Sequential isolation of biologically active compounds by metal-chelate affinity chromatography followed by azoadsorbent affinity chromatography allowed us to obtain highly purified products. The yields of superoxide dismutase and ajmaline were 180 mg/kg biomass and 16.5 g/kg dry weight, respectively.
authors.affiliation: St. Petersburg State Chemico-Pharmaceutical Academy, St. Petersburg, 197376 Russia.
authors.forename: N V
authors.initials: NV
authors.lastname: Kirillova
authors.forename: M G
authors.initials: MG
authors.lastname: Smirnova
authors.forename: V P
authors.initials: VP
authors.lastname: Komov
mesh_terms: D000404:Ajmaline / Q000302:isolation & purification*; D000889:Anti-Arrhythmia Agents / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D010946:Plants, Medicinal; D011926:Rauwolfia / Q000201:enzymology / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000302:isolation & purification*
chemical_list: D000889:Anti-Arrhythmia Agents; D000404:Ajmaline; D013482:Superoxide Dismutase