title: Potentiation by methyl pyruvate of GLP-1 insulinotropic action in normal rats.
abstract: Methyl pyruvate (MP) stimulates insulin release both in vivo and in vitro. The present study aims at investigating whether MP is also able to enhance the B-cell secretory response to glucagon-like peptide 1 (GLP-1). In anaesthetized rats receiving a primed constant infusion of MP, the ester augmented plasma insulin concentration before GLP-1 injection and potentiated the insulinotropic action of the intestinal hormone. MP infusion also augmented plasma D-glucose concentration, whether in the absence or presence of GLP-1. A further rise in plasma D-glucose concentration was observed when the infusion of MP was halted, this coinciding with a fall in plasma insulin concentration. Whilst documenting that MP indeed enhances the B-cell secretory response to GLP-1, these findings do not suggest that MP is an appropriate tool for optimizing the hypoglycaemic action of the enteric hormone in the treatment of type-2 diabetes mellitus.
authors.affiliation: Fundación Jiménez Díaz, Madrid, Spain.
authors.forename: I
authors.initials: I
authors.lastname: Valverde
authors.forename: J
authors.initials: J
authors.lastname: Cancelas
authors.forename: M L
authors.initials: ML
authors.lastname: Villanueva-Peñacarrillo
authors.forename: W J
authors.initials: WJ
authors.lastname: Malaisse
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism; D001786:Blood Glucose / Q000378:metabolism; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism; D005934:Glucagon / Q000378:metabolism* / Q000494:pharmacology; D052216:Glucagon-Like Peptide 1; D005947:Glucose / Q000378:metabolism; D007003:Hypoglycemia / Q000235:genetics; D007328:Insulin / Q000097:blood / Q000378:metabolism*; D008297:Male; D010446:Peptide Fragments / Q000378:metabolism* / Q000494:pharmacology; D011498:Protein Precursors / Q000378:metabolism* / Q000494:pharmacology; D011773:Pyruvates / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
chemical_list: D001786:Blood Glucose; D007328:Insulin; D010446:Peptide Fragments; D011498:Protein Precursors; D011773:Pyruvates; C104813:methyl pyruvate; D052216:Glucagon-Like Peptide 1; D005934:Glucagon; D005947:Glucose