title: LI-cadherin gene expression during mouse intestinal development.
abstract: LI-cadherin (Liver-Intestine cadherin) is a member of a subclass (7-D cadherins) within the cadherin superfamily. Although its cellular function as a cell-cell adhesion molecule has been demonstrated in cell culture studies, its physiological function still needs to be explored in the intact organism. After isolating the cDNA for mouse LI-cadherin, we generated specific antibodies against the overexpressed protein and studied its expression pattern in adult mouse tissues and mouse embryos. The mouse LI-cadherin sequence is 91% identical to the sequence of rat LI-cadherin and exhibits the same structural features described for rat LI-cadherin. In mouse adult tissue, LI-cadherin is expressed in the intestine and in small amounts in the spleen. In contrast to rat, Mouse LI-cadherin was not expressed in liver. During mouse embryogenesis, LI-cadherin expression begins at embryonic day 12.5. With the exception of transient expression in the urogenital sinus and the common bile duct on day 13.5, LI-cadherin was found exclusively in the intestinal epithelium. Its expression coincides with the formation of intestinal villi, a developmental stage that includes major tissue remodeling, growth, and differentiation. LI-cadherin is expressed along the entire anterior-posterior axis of the developing intestine as well as along the entire villus axis once villi begin to form. LI-cadherin occupies all cell surfaces of the deeper layers of the epithelium, distributing to basolateral surfaces only in the cells of the outer epithelial layer. LI-cadherin was found to be always co-expressed with E-cadherin.
authors.affiliation: Institut für Laboratoriumsmedizin und Pathobiochemie, Charite, Campus Virchow-Klinikum, Humboldt-Universität zu Berlin, Hindenburgdamm 30, 12200 Berlin, Germany. bmangres@clontech.com
authors.forename: B
authors.initials: B
authors.lastname: Angres
authors.forename: L
authors.initials: L
authors.lastname: Kim
authors.forename: R
authors.initials: R
authors.lastname: Jung
authors.forename: R
authors.initials: R
authors.lastname: Gessner
authors.forename: R
authors.initials: R
authors.lastname: Tauber
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001652:Bile Ducts / Q000378:metabolism; D015152:Blotting, Northern; D015153:Blotting, Western; D015820:Cadherins; D002352:Carrier Proteins / Q000096:biosynthesis*; D002454:Cell Differentiation; D002455:Cell Division; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004847:Epithelial Cells / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D015151:Immunoblotting; D007422:Intestines / Q000196:embryology*; D008099:Liver / Q000378:metabolism; D026901:Membrane Transport Proteins; D051379:Mice; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D013154:Spleen / Q000378:metabolism; D013997:Time Factors; D014018:Tissue Distribution; D014566:Urogenital System / Q000378:metabolism
chemical_list: D015820:Cadherins; D002352:Carrier Proteins; C490585:Cdh17 protein, mouse; C490586:Cdh17 protein, rat; D018076:DNA, Complementary; D026901:Membrane Transport Proteins; D012333:RNA, Messenger; C067802:intestinal peptide-proton cotransporter