[Rapid effect of glucocorticoid on excitatory postsynaptic potential of B cells in bullfrog paravertebral ganglia].
In the present study, the rapid effect of hydrocortisone 21-hemisuccinate (F-suc) on the amplitude of f-EPSP of B cells in isolated bullfrog sympathetic ganglia was examined with the use of intracellular recording technique. It was found that the amplitude of f-EPSP of B cells was decreased by F-suc, with a latency less than 3 min after the beginning of perfusion of F-suc. The inhibition of F-suc was concentration-dependent and could be blocked by the antagonist of intracellular glucocorticoid receptor RU38486, but not by the protein synthesis inhibitor actidione. These findings suggest that the inhibition of f-EPSP of B cells by F-suc is probably mediated through a nongenomic mechanism.
Ma
B
B
Department of Physiology, Second Military Medical University, Shanghai 200433.
Yi
B D
BD
Xing
B R
BR
D000818:Animals; D001402:B-Lymphocytes / Q000502:physiology; D019706:Excitatory Postsynaptic Potentials / Q000187:drug effects*; D005260:Female; D005726:Ganglia, Parasympathetic / Q000166:cytology / Q000502:physiology*; D006854:Hydrocortisone / Q000031:analogs & derivatives* / Q000494:pharmacology*; D066298:In Vitro Techniques; D008297:Male; D011892:Rana catesbeiana
C007133:hydrocortisone hemisuccinate; D006854:Hydrocortisone