Semipreparative capillary electrochromatography.
Capillaries with inner diameters of 550 microm have successfully been packed with 1.5-microm octadecyl silica particles using frits made of macroporous polymers by the UV photopolymerization of a solution of glycidyl methacrylate and trimethylolpropane trimethacrylate. This type of frit is found superior to one made of low-melting point poly(styrene-co-divinylbenzene) beads. Bubble formation is not observed to occur within these capillary columns under our experimental conditions. Separations can be achieved with sample injection volumes as high as 1 microL. To demonstrate its semipreparative use, a mixture of 500 nL of taxol (20 mM) and its precursor, baccatin III (30 mM), is separated using such a column with a Tris buffer.
Chen
J R
JR
Department of Chemistry, Stanford University, California 94305-5080, USA.
Zare
R N
RN
Peters
E C
EC
Svec
F
F
Frechét
J J
JJ
D000470:Alkaloids / Q000737:chemistry / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D019075:Electrophoresis, Capillary; D008689:Methacrylates / Q000737:chemistry; D017239:Paclitaxel / Q000737:chemistry / Q000302:isolation & purification; D011108:Polymers / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry; D043823:Taxoids
D000470:Alkaloids; D008689:Methacrylates; D011108:Polymers; D043823:Taxoids; C073950:baccatin III; D012822:Silicon Dioxide; D017239:Paclitaxel