title: Partial purification and characterization of the short-chain prenyltransferases, gernayl diphospate synthase and farnesyl diphosphate synthase, from Abies grandis (grand fir).
abstract: In the conifer Abies grandis (grand fir), a secreted oleoresin rich in mono-, sesqui-, and diterpenes serves as a constitutive and induced defense against insects and pathogenic fungi. Geranyl diphosphate (GPP) and farnesyl diphosphate (FPP) synthase, two enzymes which form the principal precursors of the oleoresin mono- and sesquiterpenes, were isolated from the stems of 2-year-old grand fir saplings. These enzymes were partially purified by sequential chromatography on DEAE-Sepharose, Mono-Q, and phenyl-Sepharose to remove competing phosphohydrolase and isopentenyl diphosphate (IPP) isomerase activities. GPP and FPP synthase formed GPP and E,E-FPP, respectively, as the sole products of the enzymatic condensation of IPP and dimethylallyl diphosphate (DMAPP). The properties of both enzymes are broadly similar to those of other prenyltransferases. The apparent native molecular masses are 54 +/- 3 kDa for GPP synthase and 110 +/- 6 kDa fo
authors.affiliation: Max-Planck Institute of Chemical Ecology, Jena, Germany.
authors.forename: D
authors.initials: D
authors.lastname: Tholl
authors.forename: R
authors.initials: R
authors.lastname: Croteau
authors.forename: J
authors.initials: J
authors.lastname: Gershenzon
mesh_terms: D019883:Alkyl and Aryl Transferases / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D002384:Catalysis / Q000187:drug effects; D002413:Cations, Divalent / Q000494:pharmacology; D002849:Chromatography, Gas; D002852:Chromatography, Ion Exchange; D003067:Coenzymes / Q000494:pharmacology; D032462:Cycadopsida / Q000201:enzymology*; D004790:Enzyme Induction; D051231:Farnesyltranstransferase; D019753:Intramolecular Lyases / Q000378:metabolism; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology; D008970:Molecular Weight; D010935:Plant Diseases; D018547:Plant Stems / Q000201:enzymology; D011106:Polyisoprenyl Phosphates / Q000494:pharmacology; D012717:Sesquiterpenes
chemical_list: D002413:Cations, Divalent; D003067:Coenzymes; D011106:Polyisoprenyl Phosphates; D012717:Sesquiterpenes; D008345:Manganese; C004808:farnesyl pyrophosphate; D019883:Alkyl and Aryl Transferases; D051231:Farnesyltranstransferase; D019753:Intramolecular Lyases; C040265:pinene cyclase I; D008274:Magnesium