title: Glyt-1 expression in cultured human Müller cells and intact retinae.
abstract: In this study, we demonstrate that Müller cells cultured from human retinas are capable of strongly expressing the glycine transporter Glyt-1 as assessed by immunocytochemistry. By contrast, intact normal and pathological human retinas exhibit Glyt-1 immunoreactivity only in neurons. These data suggest that Glyt-1 expression in cultured Müller cells is an epiphenomenon associated with culturing in vitro, rather than a normal physiological or even pathophysiological phenomenon in vivo.
authors.affiliation: Department of Physiology and Pharmacology, University of Queensland, Brisbane, Queensland, Australia.
authors.forename: P
authors.initials: P
authors.lastname: Reye
authors.forename: P
authors.initials: P
authors.lastname: Penfold
authors.forename: D V
authors.initials: DV
authors.lastname: Pow
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D026921:Amino Acid Transport Systems, Neutral; D002352:Carrier Proteins / Q000378:metabolism*; D002478:Cells, Cultured; D005260:Female; D050487:Glycine Plasma Membrane Transport Proteins; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D012016:Reference Values; D012160:Retina / Q000166:cytology* / Q000378:metabolism*; D012164:Retinal Diseases / Q000378:metabolism
chemical_list: D026921:Amino Acid Transport Systems, Neutral; D002352:Carrier Proteins; D050487:Glycine Plasma Membrane Transport Proteins; C493268:SLC6A9 protein, human