An alternative open reading frame of the human macrophage colony-stimulating factor gene is independently translated and codes for an antigenic peptide of 14 amino acids recognized by tumor-infiltrating CD8 T lymphocytes.
We show that cytotoxic T lymphocytes (CTLs) infiltrating a kidney tumor recognize a peptide encoded by an alternative open reading frame (ORF) of the macrophage colony-stimulating factor (M-CSF) gene. Remarkably, this alternative ORF, which is translated in many tumors concurrently with the major ORF, is also translated in some tissues that do not produce M-CSF, such as liver and kidney. Such a dissociation of the translation of two overlapping ORFs from the same gene is unexpected. The antigenic peptide encoded by the alternative ORF is presented by human histocompatibility leukocyte antigen (HLA)-B*3501 and has a length of 14 residues. Peptide elution indicated that tumor cells naturally present this 14 mer, which is the longest peptide known to be recognized by CTLs. Binding studies of peptide analogues suggest that it binds by its two extremities and bulges out of the HLA groove to compensate for its length.
Probst-Kepper
M
M
Ludwig Institute for Cancer Research and Cellular Genetics Unit, Université Catholique de Louvain, Brussels 1200, Belgium.
Stroobant
V
V
Kridel
R
R
Gaugler
B
B
Landry
C
C
Brasseur
F
F
Cosyns
J P
JP
Weynand
B
B
Boon
T
T
Van Den Eynde
B J
BJ
D000595:Amino Acid Sequence; D000951:Antigens, Neoplasm / Q000235:genetics* / Q000276:immunology; D001483:Base Sequence; D002292:Carcinoma, Renal Cell / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D015797:HLA-B35 Antigen; D006801:Humans; D007680:Kidney Neoplasms; D016246:Lymphocytes, Tumor-Infiltrating / Q000276:immunology*; D016173:Macrophage Colony-Stimulating Factor / Q000235:genetics* / Q000276:immunology; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010455:Peptides / Q000235:genetics* / Q000276:immunology; D014176:Protein Biosynthesis; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D000951:Antigens, Neoplasm; D015797:HLA-B35 Antigen; D010455:Peptides; D016173:Macrophage Colony-Stimulating Factor