Normal-phase high-performance liquid chromatographic determination and identification of aldrin, dieldrin, and DDTs in eggs.
A method for the routine monitoring of residual aldrin, dieldrin, pp'-DDT, op'-DDT, pp'-DDE, and pp'-DDD in eggs by high-performance liquid chromatography (HPLC) with a photo-diode array (PDA) detector is described. The lipids extracted from a whole egg are cleaned by a solid-phase extraction (SPE) ISOLUTE NH2 column with a diethyl ether-hexane (5:95, v/v) eluent. The HPLC separation is carried out using a normal-phase (LiChrosorb NH2) column, a heptane-hexane eluent (97:3, v/v), and a PDA detector. The average recoveries from fortified target compounds (0.1, 0.2, 0.3, and 0.4 microg/g, respectively) are > or = 83%, with standard deviations (SDs) between 2 and 5%. The interassay variabilities and their SDs are < or = 4.8% and < or =0.7%, with intra-assay variabilities of 2.1-3.3%. The limits of determination for these compounds range from 0.04 to 0.08 microg/g.
Furusawa
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N
Faculty of Human Life Science, Osaka City University, Japan.
D000452:Aldrin / Q000032:analysis*; D000818:Animals; D002645:Chickens; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003634:DDT / Q000032:analysis*; D004026:Dieldrin / Q000032:analysis*; D004531:Eggs / Q000032:analysis*; D005504:Food Analysis; D007306:Insecticides / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
D007306:Insecticides; D003634:DDT; D004026:Dieldrin; D000452:Aldrin