[Depolymerization of chitosan by chinolytic complex from Bacillus sp. 739].
Low-molecular-weight (3-6 kDa) water-soluble chitosan was obtained by enzymatic depolymerization. Hydrolysis of crab chitosan was induced by O-glycoside hydrolase (EC 3.2.1), an extracellular chitinolytic complex from Bacillus sp. 739. The optimum conditions for hydrolysis were found (sodium-acetate buffer, pH 5.2; 55 degrees C; an enzyme/substrate ratio 4 U/g chitosan; 1 h).
Il'ina
A V
AV
Bioinzheneriya Center, Russian Academy of Sciences, Moscow, 117312 Russia.
Varlamov
V P
VP
Melent'ev
A I
AI
Aktuganov
G E
GE
D000118:Acetylglucosaminidase / Q000378:metabolism*; D001407:Bacillus / Q000201:enzymology*; D002686:Chitin / Q000031:analogs & derivatives / Q000378:metabolism*; D002688:Chitinases / Q000378:metabolism*; D048271:Chitosan; D006868:Hydrolysis
D002686:Chitin; D048271:Chitosan; D002688:Chitinases; D000118:Acetylglucosaminidase