Systematic approaches to mouse mutagenesis.
A major challenge in post-genomics is the systematic determination of mammalian gene function. A variety of mouse mutagenesis technologies, both gene- and phenotype-driven, are being used to underpin systematic and comprehensive approaches to mammalian gene function studies. Recently, a number of centres have completed large-scale ENU mutagenesis programmes that employ a phenotype-driven approach to the generation of mouse mutants. The use of ENU mutagenesis represents a powerful and efficient approach to mammalian gene-function studies, but many parallel developments are needed in downstream technologies to properly harness the new enlarged mouse-mutant resources that are being created.
Brown
S D
SD
MRC Mammalian Genetics Unit and UK Mouse Genome Centre, OX11 ORD, Harwell, UK. s.brown@har.mrc.ac.uk
Balling
R
R
D000818:Animals; D005038:Ethylnitrosourea; D014644:Genetic Variation; D006801:Humans; D051379:Mice; D008817:Mice, Mutant Strains / Q000235:genetics*; D016296:Mutagenesis; D010641:Phenotype
D005038:Ethylnitrosourea