Development of 90 new bovine microsatellite loci.
Polymorphic genetic markers are important tools in the construction of comprehensive genetic maps. This paper reports on the isolation and characterization of 90 new bovine microsatellite (ms) loci from enriched genomic libraries. The sequence of one clone (locus MNB-85) showed significant similarity to an intron of the human promyelocytic leukemia zinc finger protein (PLZF) gene. Screening of bovine and porcine somatic cell panels places the bovine PLZF homolog on BTA-15 and the porcine PLZF homolog on SSC-9. The 90 new microsatellite loci increase the number of microsatellites available for cattle by >5%.
Reed
K M
KM
Department of Veterinary PathoBiology, College of Veterinary Medicine, University of Minnesota, St. Paul 55108, USA.
Mendoza
K M
KM
Beattie
C W
CW
D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000235:genetics*; D003001:Cloning, Molecular; D004277:DNA, Single-Stranded; D004268:DNA-Binding Proteins / Q000235:genetics*; D015723:Gene Library; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D013552:Swine / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics*
D004277:DNA, Single-Stranded; D004268:DNA-Binding Proteins; D014157:Transcription Factors