[DNA from various tissues of the Far Eastern larch and its applicability for RAPD assay].
We studied applicability of RAPD assay for investigating genetic variability of far eastern larches. Techniques to isolate DNA from larch vegetative organs (needles, young shoots and stems) and megagametophytes were selected, and conditions of polymerase chain reaction were optimized. Fifty out of 84 random primers proved efficient, and 22 and 12 primers initiated weak and no synthesis, respectively. The tested primers worked efficiently on the DNA isolated from both diploid and haploid tissues.
Sazonova
I Iu
IIu
Institute of Biology and Soil Sciences, Far East Division, Russian Academy of Sciences, pr. Stoletiya Vladivostoka 159, Vladivostok, 690022 Russia.
Kozyrenko
M M
MM
Artiukova
E V
EV
Reunova
G D
GD
Zhuravlev
Iu N
IuN
D018744:DNA, Plant / Q000032:analysis*; D005202:Asia, Eastern; D018514:Plant Structures / Q000737:chemistry*; D019105:Random Amplified Polymorphic DNA Technique; D012800:Siberia; D014197:Trees / Q000737:chemistry*
D018744:DNA, Plant