Cytokine- and microbially induced sleep responses of interleukin-10 deficient mice.
Interleukin (IL)-1 and tumor necrosis factor (TNF) promote slow-wave sleep (SWS), whereas IL-10 inhibits the synthesis of IL-1 and TNF and promotes waking. We evaluated the impact of endogenous IL-10 on sleep-wake behavior by studying mice that lack a functional IL-10 gene. Under baseline conditions, C57BL/6-IL-10 knockout (KO) mice spent more time in SWS during the dark phase of the light-dark cycle than did genetically intact C57BL/6 mice. The two strains of mice showed generally comparable responses to treatment with IL-1, IL-10, or influenza virus, but differed in their responses to lipopolysaccharide (LPS). In IL-10 KO mice, LPS induced an initial transient increase and a subsequent prolonged decrease in SWS, as well as profound hypothermia. These responses were not observed in LPS-treated C57BL/6 mice. These data demonstrate that in the absence of endogenous IL-10, spontaneous SWS is increased and the impact of LPS on vigilance states is altered. Collectively, these observations support a role for IL-10 in sleep regulation and provide further evidence for the involvement of cytokines in the regulation of sleep.
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Department of Pharmacology, Southern Illinois University School of Medicine, Springfield, Illinois 62794, USA.
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D000818:Animals; D001831:Body Temperature; D016207:Cytokines / Q000494:pharmacology*; D007375:Interleukin-1 / Q000494:pharmacology; D016753:Interleukin-10 / Q000172:deficiency* / Q000235:genetics; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout / Q000235:genetics; D009043:Motor Activity / Q000502:physiology; D009976:Orthomyxoviridae Infections / Q000503:physiopathology; D012890:Sleep / Q000187:drug effects* / Q000502:physiology*
D016207:Cytokines; D007375:Interleukin-1; D008070:Lipopolysaccharides; D016753:Interleukin-10