[Effects of mifepristone on choriocarcinoma cell line JAR in vitro].
OBJECTIVE
To investigate the effects of mifepristone on cell proliferation and hCG secretion of human choriocarcinoma cell line JAR and the cytotoxic effects of mifepristone on this cell line.


METHODS
The cytotoxic effects of four different concentration of mifepristone (1,5,10,20 mumol/L) were assessed with methyl thiazolyl tetrazolium (MTT) colorimetric assay. The expression of nuclear proliferative antigen Ki-67 and the amount of hCG secreation in JAR cells were detected with avidin-biotin complex method, immuno-histochemical method and enzyme-linked immunosorbent assay (ELISA) respectively.


RESULTS
There were dose-dependent cytotoxic effects on JAR cells in four different concentrations of mifepristone. This effect plateaued at 20 mumol/L. When the JAR cells were exposed to 5 mumol/L mifepristone for 72 h, the expression of Ki-67 antigen was declined and the amount of hCG secretion was decreased significantly at the third day.


CONCLUSIONS
In vitro, mifepristone has cytostatic and cytotoxic effects on JAR cell line and may inhibit the secretion of hCG in JAR cells.
Sun
L
L
Second Affiliated Hospital, Henan Medical University, Zhengzhou 450003.
Zheng
Y
Y
Wang
C
C
D002455:Cell Division / Q000187:drug effects; D002822:Choriocarcinoma / Q000473:pathology*; D006063:Chorionic Gonadotropin / Q000378:metabolism; D005260:Female; D006801:Humans; D019394:Ki-67 Antigen / Q000032:analysis; D015735:Mifepristone / Q000494:pharmacology*; D011247:Pregnancy; D014407:Tumor Cells, Cultured / Q000187:drug effects; D014594:Uterine Neoplasms / Q000473:pathology*
D006063:Chorionic Gonadotropin; D019394:Ki-67 Antigen; D015735:Mifepristone