Regiospecific dechlorination of spiked tetra- and trichlorodibenzo-p-dioxins by anaerobic bacteria from PCDD/F-contaminated Spittelwasser sediments.
Samples were taken from sediment of the River Spittelwasser (district Bitterfeld, Germany), which is highly polluted with PCDD/Fs and other chloroorganic compounds. The sediment cores were separated into 10-20 cm thick layers, spiked with 50 microM of 1,2,3,4-tetrachlorodibenzo-p-dioxin and incubated for 8 months under anaerobic conditions in the presence of cosubstrates. Reductive dechlorination of the tetrachlorinated congener and formation of tri- and dichlorinated products were observed in all biologically active incubations. Analysis of subcultures spiked with 1,2,3- and 1,2,4-trichlorodibenzo-p-dioxin, respectively, revealed two different dechlorination pathways within the sediment cores. Pathway M was characterized by the simultaneous dechlorination of peri- and lateralchlorine atoms, whereas sequence SP was restricted to the dechlorination at positions flanked by chlorine atoms on both sides.
Bunge
M
M
Institute of Microbiology, Martin Luther University, Halle, Germany. m.bunge@mikrobiologie.uni-halle.de
Ballerstedt
H
H
Lechner
U
U
D001421:Bacteria, Anaerobic / Q000378:metabolism*; D001572:Benzofurans / Q000378:metabolism*; D001673:Biodegradation, Environmental; D017606:Chlorine Compounds / Q000378:metabolism; D000072338:Dibenzofurans, Polychlorinated; D004784:Environmental Monitoring; D019015:Geologic Sediments / Q000737:chemistry / Q000382:microbiology; D000072317:Polychlorinated Dibenzodioxins / Q000031:analogs & derivatives / Q000378:metabolism*; D012989:Soil Pollutants / Q000378:metabolism*
D001572:Benzofurans; D017606:Chlorine Compounds; D000072338:Dibenzofurans, Polychlorinated; D000072317:Polychlorinated Dibenzodioxins; D012989:Soil Pollutants