Construction of a BAC library derived from the inbred Hd-rR strain of the teleost fish, Oryzias latipes.
A large insert genomic bacterial artificial chromosome (BAC) library was constructed from the inbred Hd-rR strain of the medaka, Oryzias latipes. Approximately 92,000 clones were gridded on high-density replica filters. Insert analysis of randomly selected clones indicated a mean insert size of 210 kb and predicted a 24 times coverage of the medaka genome. The library was hybridized with a single locus DNA fragment, and the resulting positive clones were characterized and shown to be compatible with a 24-fold redundant library. This first large insert genomic library of the medaka should increase the speed of genomic analyses for this fish species.
Matsuda
M
M
Laboratory of Reproductive Biology, National Institute for Basic Biology, Okazaki, Japan.
Kawato
N
N
Asakawa
S
S
Shimizu
N
N
Nagahama
Y
Y
Hamaguchi
S
S
Sakaizumi
M
M
Hori
H
H
D000818:Animals; D017849:Animals, Inbred Strains; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics; D015723:Gene Library; D005821:Genetic Techniques; D009990:Oryzias / Q000235:genetics*