Green tea catechins enhance tumor development in the colon without effects in the lung or thyroid after pretreatment with 1,2-Dimethylhydrazine or 2,2'-dihydroxy-di-n-propylnitrosamine in male F344 rats.
Modifying effects of green tea catechins (GTCs) on the post-initiation stage of colon, lung and thyroid carcinogenesis were examined in F344 male rats. Groups of 20 animals were given subcutaneous injections of 40 mg/kg body wt of 1,2-dimethylhydrazine twice a week for 2 weeks or oral administration of 0.1% 2,2'-dihydroxy-di-n-propylnitrosamine (DHPN) in the drinking water for 2 weeks for initiation. They then received diet containing 1 or 0.1% green tea catechin or basal diet alone for 33 weeks. Histopathological examination after final sacrifice showed that although total incidence and multiplicity of colon tumors were not significantly different from controls, values for colon adenomas were decreased while those for carcinomas and the average size of tumors were significantly increased in the 0.1% GTC group. A similar tendency was observed for the 1% GTC group. Incidences and/or multiplicity of lung hyperplasia and tumors, and thyroid lesions did not significantly vary among the DHPN-treated groups. These results indicate that GTCs do not inhibit, but rather may enhance colon carcinogenesis, while not influencing lung and thyroid carcinogenesis under the present experimental conditions.
Hirose
M
M
Division of Pathology, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, 158-8501, Tokyo, Japan. m-hirose@nihs.go.jp
Hoshiya
T
T
Mizoguchi
Y
Y
Nakamura
A
A
Akagi
K
K
Shirai
T
T
D019813:1,2-Dimethylhydrazine; D000236:Adenoma / Q000139:chemically induced*; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002273:Carcinogens; D002277:Carcinoma / Q000139:chemically induced*; D002392:Catechin / Q000627:therapeutic use*; D003110:Colonic Neoplasms / Q000139:chemically induced*; D008175:Lung Neoplasms / Q000139:chemically induced*; D008297:Male; D009602:Nitrosamines; D009929:Organ Size / Q000187:drug effects; D008517:Phytotherapy; D051381:Rats; D011916:Rats, Inbred F344; D013662:Tea / Q000627:therapeutic use*; D013964:Thyroid Neoplasms / Q000139:chemically induced*
D002273:Carcinogens; D009602:Nitrosamines; D013662:Tea; C012457:diisopropanolnitrosamine; D002392:Catechin; D019813:1,2-Dimethylhydrazine