[Effect of aldosterone on the secretion of endothelin by ventricular fibroblasts].
Using cell culture, radioimmunoassay for endothelin and RT-PCR, the effect of aldosterone on the endothelin secretion of ventricular fibroblasts was studied. The results showed that aldosterone (1 x 10(-7) mol/L) promoted the expression of ppET-1 mRNA, which began to increase in 2 hours and attained the highest level in 4 hours, thereafter decreased; aldosterone increased the endothelin level in ventricular fibroblasts and fibroblast conditioned growth medium (FCGM) as well, which was blocked by spironolactone (1 x 10(-6) mol/L), an aldosterone receptor antagonist. The results suggest that aldosterone can increase endothelin secretion by ventricular fibroblasts, which can be inhibited by its receptor antagonist spironolactone.
Gong
S Z
SZ
Department of Physiology, Sun Yat-Sen University of Medical Sciences, Guangzhou 510089.
Liu
P Q
PQ
Lu
W
W
Wang
T H
TH
Fu
S G
SG
Tan
Z
Z
Pan
J Y
JY
D000450:Aldosterone / Q000494:pharmacology*; D000818:Animals; D002478:Cells, Cultured; D016232:Endothelins / Q000378:metabolism*; D005260:Female; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism*; D006352:Heart Ventricles / Q000166:cytology; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
D016232:Endothelins; D000450:Aldosterone