Crystallization and preliminary crystallographic data of fructose-1,6-bisphosphatase from human muscle.
The enzyme human muscle fructose-1,6-bisphosphatase, which plays a critical role in gluconeogenesis, has been crystallized in the presence of 2-propanol, polyethylene glycol and magnesium chloride at pH 7.5. The space group was determined to be P4(2)2(1)2, with unit-cell parameters a = b = 73.57, c = 146.50 A, alpha = beta = lambda = 90 degrees and one subunit in the asymmetric unit. A 99.6% complete data set to 2.04 A has been collected at the National Synchrotron Light Source.
Zhu
D W
DW
The Laboratory of Molecular Endocrinology, CHUL Research Center and Laval University, Quebec G1V 4G2, Canada.
Xu
G J
GJ
Rehse
P H
PH
Shi
R
R
Zhao
F K
FK
Lin
S X
SX
D019840:2-Propanol / Q000737:chemistry; D003460:Crystallization; D018360:Crystallography, X-Ray; D006597:Fructose-Bisphosphatase / Q000737:chemistry*; D006801:Humans; D008274:Magnesium / Q000737:chemistry; D009132:Muscles / Q000201:enzymology*; D011092:Polyethylene Glycols / Q000737:chemistry; D011487:Protein Conformation
D011092:Polyethylene Glycols; D006597:Fructose-Bisphosphatase; D008274:Magnesium; D019840:2-Propanol