title: The fetal liver counterpart of adult common lymphoid progenitors gives rise to all lymphoid lineages, CD45+CD4+CD3- cells, as well as macrophages.
abstract: We identified an IL-7Ralpha(+)Sca-1(low)c-Kit(low) population in E14 fetal liver, which is the phenotypical analog of common lymphoid progenitors (CLP) in adult bone marrow. After transfer into newborn mice, the IL-7Ralpha(+)Sca-1(low)c-Kit(low) population rapidly differentiated into CD45(+)CD4(+)CD3(-) cells, which are candidate cells for initiating lymph node and Peyer's patch formation. In addition, this population also gave rise to B, T, NK, and CD8alpha(+) and CD8alpha(-) dendritic cells. The fetal liver precursors expressed a significantly lower level of the myeloid-suppressing transcription factor Pax-5, than adult CLP, and retained differentiation activity for macrophages in vitro. We propose that the transition from fetal liver IL-7Ralpha(+)Sca-1(low)c-Kit(low) cells to adult CLP involves a regulated restriction of their developmental potential, controlled, at least in part, by Pax-5 expression.
authors.affiliation: Department of Cell Biology and Immunology, Faculty of Medicine, Vrije Universiteit, v.d. Boechorststraat 7, 1081 BT Amsterdam, The Netherlands. r.mebius.cell@med.vu.nl
authors.forename: R E
authors.initials: RE
authors.lastname: Mebius
authors.forename: T
authors.initials: T
authors.lastname: Miyamoto
authors.forename: J
authors.initials: J
authors.lastname: Christensen
authors.forename: J
authors.initials: J
authors.lastname: Domen
authors.forename: T
authors.initials: T
authors.lastname: Cupedo
authors.forename: I L
authors.initials: IL
authors.lastname: Weissman
authors.forename: K
authors.initials: K
authors.lastname: Akashi
mesh_terms: D000818:Animals; D000831:Animals, Newborn / Q000276:immunology; D000950:Antigens, Ly / Q000096:biosynthesis; D001402:B-Lymphocytes / Q000166:cytology; D017252:CD3 Complex / Q000096:biosynthesis*; D015704:CD4 Antigens / Q000096:biosynthesis*; D002454:Cell Differentiation / Q000276:immunology; D019070:Cell Lineage / Q000276:immunology; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000637:transplantation; D015672:Erythroid Precursor Cells / Q000166:cytology; D005333:Fetus / Q000166:cytology / Q000276:immunology; D018507:Gene Expression Regulation, Developmental / Q000276:immunology; D016130:Immunophenotyping; D017493:Leukocyte Common Antigens / Q000096:biosynthesis*; D008099:Liver / Q000166:cytology / Q000196:embryology* / Q000276:immunology* / Q000378:metabolism; D016031:Liver Transplantation / Q000276:immunology; D008198:Lymph Nodes / Q000166:cytology; D016131:Lymphocyte Subsets / Q000166:cytology* / Q000378:metabolism / Q000637:transplantation; D008264:Macrophages / Q000166:cytology* / Q000378:metabolism; D008565:Membrane Proteins / Q000096:biosynthesis; D051379:Mice; D008810:Mice, Inbred C57BL; D022423:Myeloid Cells / Q000166:cytology; D019009:Proto-Oncogene Proteins c-kit / Q000096:biosynthesis; D020395:Receptors, Interleukin-7 / Q000096:biosynthesis; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology / Q000276:immunology* / Q000378:metabolism
chemical_list: D000950:Antigens, Ly; D017252:CD3 Complex; D015704:CD4 Antigens; C074650:Ly6a protein, mouse; D008565:Membrane Proteins; D020395:Receptors, Interleukin-7; C415350:interleukin-7 receptor, alpha chain; D019009:Proto-Oncogene Proteins c-kit; D017493:Leukocyte Common Antigens