title: Comparative study of in vivo and in vitro phagocytosis including germicidal capacity in Odontaster validus (Koehler, 1906) at 0 degree C.
abstract: The phagocytosis and germicidal capacity of Saccharomyces cerevisiae by phagocytic amoebocytes (PA) of the Antarctic starfish Odontaster validus were studied in vivo (after incubation periods of 1, 2, and 4 h) and in vitro (after incubation periods of 1, 2, 4, 8, and 12 h) at 0 degree C. The total number of PA and the phagocytic capacity (PC), phagocytic index (PI), and germicidal capacity (GC) of the PA were calculated. Results showed significant variability of the total PA number in different animals. There was a significant increase in PC and no significant differences in PI and GC for different in vitro incubation times. In vivo, experiments showed no significant difference of PC and PI, but there was a significant increase in GC as incubation periods increased. Comparison between in vitro and in vivo results revealed that PI and PC were significantly higher in vitro and that GC was significantly higher in vivo. The present study shows for the first time the phagocytosis and GC of an Antarctic invertebrate in vivo at low temperature (0 degree C), and the results are comparing with the available literature for echinoderms.
authors.affiliation: Instituto de Ciências Biomédicas I, Departamento de Histologia e Embriologia, University of São Paulo, Av. Prof. Lineu Prestes, 1524, Cidade Universitária, São Paulo, SP, 05508-900, Brazil. jrmcs@usp.br
authors.forename: J R
authors.initials: JR
authors.lastname: Silva
authors.forename: F J
authors.initials: FJ
authors.lastname: Hernandez-Blazquez
authors.forename: L R
authors.initials: LR
authors.lastname: Porto-Neto
authors.forename: J C
authors.initials: JC
authors.lastname: Borges
mesh_terms: D000818:Animals; D000864:Antarctic Regions; D015169:Colony Count, Microbial; D010587:Phagocytosis / Q000502:physiology*; D012441:Saccharomyces cerevisiae / Q000166:cytology; D013215:Starfish / Q000382:microbiology* / Q000502:physiology*