Enhanced expression and release of C-type natriuretic peptide in freshwater eels.
C-type natriuretic peptide (CNP) is recognized as a paracrine factor acting locally in the brain and periphery. To assess the role of CNP in teleost fish, a cDNA encoding a CNP precursor was initially cloned from the eel brain. CNP message subsequently detected by ribonuclease protection assay, using the cDNA as probe, was most abundant in the brain followed by liver, gut, gills, and heart. Expression was generally higher in freshwater (FW) than in seawater (SW) eels, but not in the brain. Plasma CNP concentration measured by a newly developed homologous radioimmunoassay for eel CNP was higher in FW than in SW eels. The CNP concentration was also higher in the heart of FW eels but not in the brain. These results show that CNP is abundantly synthesized in peripheral tissues of FW eels and secreted constitutively into the circulation. Therefore, CNP is a circulating hormone as well as a paracrine factor in eels. Together with our previous demonstration that CNP-specific receptor expression is enhanced in FW eels, it appears that CNP is a hormone important for FW adaptation. Because atrial NP (ANP) promotes SW adaptation in eels, CNP and ANP, despite high sequence identity, appear to have opposite effects on environmental adaptation of the euryhaline fish.
Takei
Y
Y
Ocean Research Institute, the University of Tokyo, Nakano-ku, Tokyo 164-8639, Japan. takei@ori.u-tokyo.ac.jp
Inoue
K
K
Ando
K
K
Ihara
T
T
Katafuchi
T
T
Kashiwagi
M
M
Hirose
S
S
D000595:Amino Acid Sequence / Q000235:genetics; D000818:Animals; D001483:Base Sequence / Q000235:genetics; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D004524:Eels / Q000378:metabolism*; D005618:Fresh Water; D008969:Molecular Sequence Data; D020098:Natriuretic Peptide, C-Type / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D009994:Osmolar Concentration; D012333:RNA, Messenger / Q000378:metabolism; D011863:Radioimmunoassay; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012260:Ribonucleases; D012623:Seawater; D014018:Tissue Distribution
D018076:DNA, Complementary; D012333:RNA, Messenger; D020098:Natriuretic Peptide, C-Type; D012260:Ribonucleases