[Cloning and sequence analysis of a pseudogene of liver regeneration augmenter in rats].
OBJECTIVE
To investigate the status of the augmenter of liver regeneration (ALR) in rat's genome.


METHODS
Polymerase chain reaction (PCR) was used to amplify the genomic DNA of rat, with a set of specific primers designed according to the cDNA sequence of ALR. The products were ligated into pGEM Teasy vector. Two positive clones were sequenced separately.


RESULTS
Two products were amplified from the rat's genome by PCR. After sequencing, one pseudogene was identified. The homology of the amino acid sequence between the ALR and its pseudogene was 88.8%.


CONCLUSIONS
ALR pseudogene is found in rat's genome, implying that there is an ALR multigene family. This finding lays a foundation for further study of ALR molecular evolution mode.
Dong
J
J
Gene Therapy Research Center, Institute of Infectious Diseases, 302 nd Hospital of PLA, Beijing 100039, China.
Cheng
J
J
Liu
Y
Y
Wang
Q
Q
Wang
G
G
Shi
S
S
Si
C
C
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry; D006133:Growth Substances / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011506:Proteins; D011544:Pseudogenes; D051381:Rats; D017208:Rats, Wistar; D050862:Oxidoreductases Acting on Sulfur Group Donors
D018076:DNA, Complementary; D006133:Growth Substances; D011506:Proteins; C088825:GFER protein, rat; D050862:Oxidoreductases Acting on Sulfur Group Donors