title: Non-penetrating vascular clips anastomosis inhibited intimal thickening under poor runoff conditions in canine autogenous vein grafts.
abstract: OBJECTIVE
  Late graft failure is still a significant problem, particularly in cases with poor runoff vessels. The main cause of late graft failure is intimal thickening of the anastomotic region. Vascular closure system (VCS) clips may provide ideal anastomosis, since they do not penetrate the wall. Therefore, we examined whether the VCS clips affect intimal thickening under poor runoff conditions in the canine autogenous vein grafts.
  
  
  METHODS
  A canine poor runoff model was prepared at both femoral veins. Four weeks after the first surgical procedure, two groups were established according to the two different methods of anastomosis employed. The right femoral vein graft was performed using polypropylene sutures, conventional surgical anastomosis (control group), while the left femoral vein graft was performed using VCS clips anastomosis (VCS group). Four weeks after grafting, the vein grafts were removed and the intimal thickening of proximal, distal anastomosis and midportion of the vein grafts were examined histologically.
  
  
  RESULTS
  In the control group, flow rate and variation were 26+/-8 ml/min and 51+/-10 dynes/cm(2), respectively. In the VCS group, the flow rate and variation were 23+/-11 ml/min and 44+/-14 dynes/cm(2), respectively. There were no significant differences between the two groups. The average value of intimal thickening of both the anastomotic region and the midportion of the vein graft in the VCS group was significantly inhibited compared to that of the control group. The number of positive cells of masson trichrome stain in the VCS group was significantly less than that of the control group.
  
  
  CONCLUSIONS
  These experiments indicate that VCS clips significantly inhibit intimal thickening under poor runoff conditions in canine autogenous vein grafts to a greater extent compared to suture-constructed anastomosis. One mechanism that may account for the decreased intimal thickening is the inhibition of the expression of transforming growth factor-beta (TGF-beta), because the number of positive cells of masson trichrome stain in the VCS group was significantly less than that of the control group.
authors.affiliation: Department of Surgery and Science, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Komori
authors.forename: T
authors.initials: T
authors.lastname: Shoji
authors.forename: T
authors.initials: T
authors.lastname: Furuyama
authors.forename: Y
authors.initials: Y
authors.lastname: Yonemitsu
authors.forename: E
authors.initials: E
authors.lastname: Mori
authors.forename: T
authors.initials: T
authors.lastname: Yamaoka
authors.forename: T
authors.initials: T
authors.lastname: Matsumoto
authors.forename: K
authors.initials: K
authors.lastname: Sugimachi
mesh_terms: D000818:Animals; D001166:Arteriovenous Shunt, Surgical / Q000295:instrumentation*; D001783:Blood Flow Velocity; D004195:Disease Models, Animal; D004285:Dogs; D005268:Femoral Vein / Q000473:pathology* / Q000637:transplantation*; D006965:Hyperplasia; D018709:Statistics, Nonparametric; D013525:Surgical Instruments; D014182:Transplantation, Autologous; D017539:Tunica Intima / Q000473:pathology*