title: Allelic structure and distribution of two STR loci, D8S580 and D22S442, in the Japanese population.
abstract: The allelic frequency and structural characteristics of two STR loci D8S580 and D22S442 were investigated using blood samples from 143 unrelated healthy Japanese individuals. Thirty-eight alleles in D8S580 locus and 13 alleles in D22S442 locus were identified. The discrimination power, heterozygosity, and the polymorphic information content of those loci displayed high values (0.98, 0.88, and 0.87 in D8S580 and 0.97, 0.86 and 0.85 in D22S442), and their frequency distributions met Hardy-Weinberg equilibrium expectations. The allelic pattern of D8S580 was complex and differentiated into three groups (group I: alleles 184-194bp; group II: alleles 203-223, 235, 239, 243, 252 and 255bp; group III: alleles 227-286bp). Most of their alleles contained five categories of repeat units (A: aaaag; B: aaag; C: aagg; D: caag; E: agaa). On the other hand, D22S442 contained only two types of repeat units (A: agga; B: aggg). The present study, hence, proves that both D8S580 and D22S442 are highly polymorphic and represent stable genetic markers applicable to forensic investigations.
authors.affiliation: Department of Legal Medicine, Shinshu University School of Medicine, Asahi 3-1-1, 390-8621, Matsumoto, Japan. otamasao@sch.md.shinshu-u.ac.jp
authors.forename: M
authors.initials: M
authors.lastname: Ota
authors.forename: Y
authors.initials: Y
authors.lastname: Katsuyama
authors.forename: C
authors.initials: C
authors.lastname: Liu
authors.forename: K
authors.initials: K
authors.lastname: Takayanagi
authors.forename: H
authors.initials: H
authors.lastname: Asamura
authors.forename: H
authors.initials: H
authors.lastname: Fukushima
mesh_terms: D000483:Alleles; D001782:Blood Donors; D016172:DNA Fingerprinting / Q000379:methods* / Q000592:standards; D016002:Discriminant Analysis; D005787:Gene Frequency / Q000235:genetics*; D005819:Genetic Markers / Q000235:genetics; D006579:Heterozygote; D006801:Humans; D007564:Japan; D018598:Minisatellite Repeats / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000592:standards; D011110:Polymorphism, Genetic / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000379:methods* / Q000592:standards
chemical_list: D005819:Genetic Markers