Ischaemic preconditioning in the rat brain: a longitudinal magnetic resonance imaging (MRI) study.
Ischaemic preconditioning in rats was studied using MRI. Ischaemic preconditioning was induced, using an intraluminal filament method, by 30 min middle cerebral artery occlusion (MCAO), and imaged 24 h later. The secondary insult of 100 min MCAO was induced 3 days following preconditioning and imaged 24 and 72 h later. Twenty-four hours following ischaemic preconditioning most rats showed small sub-cortical hyperintense regions not seen in sham-preconditioned rats. Twenty-four hours and 72 h following the secondary insult preconditioned animals showed significantly smaller lesions (24 h = 112 +/- 31 mm(3), mean +/- standard error; 72 h = 80 +/- 35 mm(3)), which were confined to the striatum, than controls (24 h = 234 +/- 32 mm(3), p = 0.026; 72 h = 275 +/- 37 mm(3), p = 0.003). In addition during lesion maturation from 24 to 72 h post-secondary MCAO, preconditioned rats displayed an average reduction in lesion size as measured by MRI whereas sham-preconditioned rats displayed increases in lesion size; this is the first report of such differential lesion volume evolution in cerebral ischaemic preconditioning.
Mullins
P G
PG
Safety Assessment, GlaxoSmithKline, The Frythe, Welwyn, Herts AL6 9AR, UK.
Reid
D G
DG
Hockings
P D
PD
Hadingham
S J
SJ
Campbell
C A
CA
Chalk
J B
JB
Doddrell
D M
DM
D000818:Animals; D002546:Ischemic Attack, Transient / Q000473:pathology*; D019194:Ischemic Preconditioning; D008137:Longitudinal Studies; D008279:Magnetic Resonance Imaging; D008297:Male; D020768:Middle Cerebral Artery; D051381:Rats; D017207:Rats, Sprague-Dawley