Detection of DAZ mRNA distribution in human testis using reverse transcription in situ PCR technique (RT-ISPCR).
Normal course of spermatogenesis reflects appropriate expression of specific genes in spermatogenic cells. In the present study, the localization of DAZ mRNA in human testis obtained from two fertile organ donors has been determined. It was established that DAZ mRNA is located in the germinal epithelium of seminiferous tubules. The most intensive accumulation of DAZ transcript was observed in primary spermatocytes. The results indicate that expression of DAZ gene begins in spermatogonia and that distribution of DAZ mRNA most likely correlates with the stage of the human seminiferous cycle.
Warchoł
J B
JB
Department of Radiobiology and Cell Biology, University of Medical Sciences, Poznań, Poland. jwarchol@usoms.poznan.pl
Augustyniak
S
S
Stecewicz
D
D
Jankowska
A
A
D000075364:Deleted in Azoospermia 1 Protein; D006801:Humans; D008297:Male; D009845:Oligospermia / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012671:Seminiferous Tubules / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure; D013090:Spermatocytes / Q000378:metabolism / Q000648:ultrastructure; D013737:Testis / Q000378:metabolism* / Q000648:ultrastructure
C095668:DAZ1 protein, human; D000075364:Deleted in Azoospermia 1 Protein; D012333:RNA, Messenger; D016601:RNA-Binding Proteins