Allelic IGF2R repression does not correlate with expression of antisense RNA in human extraembryonic tissues.
In the mouse, expression of an antisense Igf2r RNA (Air) is correlated with Igf2r repression on the paternal allele. One of the possible models for Igf2r repression could be through promoter competition or through the action of the Air RNA, in, e.g., transcriptional interference or repressor binding. These models predict the conservation of AIR RNA in human samples with monoallelic IGF2R expression and the production of AIR RNA in first-trimester human tissues. However, by strand-specific RT-PCR and by ribonuclease protection assay we have not detected any AIR RNA in first-trimester placental tissue samples, not even in samples that downregulate IGF2R expression in an allele-specific manner. This indicates that in contrast to the mouse, allelic IGF2R repression in the developing human placenta does not correlate with AIR expression.
Oudejans
C B
CB
Molecular Biology Laboratory, Department of Clinical Chemistry, University Hospital 'Vrije Universiteit', De Boelelaan 1117, Amsterdam, 1081 HV, The Netherlands. cbm.oudejans@azvu.nl
Westerman
B
B
Wouters
D
D
Gooyer
S
S
Leegwater
P A
PA
van Wijk
I J
IJ
Sleutels
F
F
D000483:Alleles; D005260:Female; D015870:Gene Expression; D005786:Gene Expression Regulation / Q000235:genetics*; D018392:Genomic Imprinting; D006801:Humans; D023282:Nuclease Protection Assays; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D016372:RNA, Antisense / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics*; D017527:Receptor, IGF Type 2 / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013379:Substrate Specificity; D014158:Transcription, Genetic
D016372:RNA, Antisense; D017527:Receptor, IGF Type 2