title: FEM1, a Fusarium oxysporum glycoprotein that is covalently linked to the cell wall matrix and is conserved in filamentous fungi.
abstract: As part of an investigation of the cell wall structure of plant pathogenic, filamentous fungi, we set out to characterize covalently bound cell wall glycoproteins (CWPs) of the tomato pathogen Fusarium oxysporum. N-terminal sequencing of an abundant 60-kDa CWP led to the cloning of the corresponding gene, which we have designated FEM1 (Fusarium extracellular matrix protein). The gene contains an ORF encoding a primary translation product of 212 amino acids, including an N-terminal 17-amino acid secretion signal sequence. Furthermore, FEM1p contains two potential N-glycosylation sites, and is rich in serine and threonine residues (29%) that could serve as O-glycosyl addition sites. At its C-terminus the protein contains a 22-amino acid sequence with the characteristics of a glycosyl-phosphatidylinositol (GPI) anchor addition signal. A mutant FEM1 protein lacking this GPI anchor addition signal is not retained in the fungal cell wall but released into the culture medium, indicating that in the wild-type protein this sequence functions to anchor the protein to the extracellular matrix. Southern analysis shows that FEM1 is present as a single-copy gene in all formae speciales of F. oxysporum tested and in F. solani. Database searches show that FEM1p homologous sequences are present in other filamentous fungi as well.
authors.affiliation: Section of Plant Pathology, Swammerdam Institute for Life Sciences, Amsterdam, The Netherlands.
authors.forename: E A
authors.initials: EA
authors.lastname: Schoffelmeer
authors.forename: J H
authors.initials: JH
authors.lastname: Vossen
authors.forename: A A
authors.initials: AA
authors.lastname: van Doorn
authors.forename: B J
authors.initials: BJ
authors.lastname: Cornelissen
authors.forename: M A
authors.initials: MA
authors.lastname: Haring
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D002473:Cell Wall / Q000378:metabolism; D003001:Cloning, Molecular; D016326:Extracellular Matrix Proteins / Q000235:genetics* / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D005670:Fusarium / Q000235:genetics* / Q000378:metabolism; D006023:Glycoproteins / Q000235:genetics* / Q000378:metabolism; D006031:Glycosylation; D017261:Glycosylphosphatidylinositols / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D016366:Open Reading Frames; D021382:Protein Sorting Signals; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment
chemical_list: D016326:Extracellular Matrix Proteins; C428243:FEM1 protein, Fusarium oxysporum; D005656:Fungal Proteins; D006023:Glycoproteins; D017261:Glycosylphosphatidylinositols; D021382:Protein Sorting Signals