[The determination of diuron and chlortoluron residues in beef and beef products by high performance liquid chromatography].
Amethod for the determination of diuron and chlortoluron in beef and beef products with high performance liquid chromatography is described. The sample was extracted with a mixture of acetonitrile and methanol (50:50, V/V). After filtration, the filtrate was defatted with petroleum ether, then water was added and further extracted with chloroform. The chloroform collected was evaporated in a rotary evaporator(45 degrees C). The residue was dissolved in acetonitrile-methanol(50:50, V/V) mixture, poured into an Al2O3 column and eluted with the same mixture. The eluate was collected for HPLC analysis. The analytical column was Selectosil C18 (5 microns, 250 mm x 4.6 mm i.d.), mobile phase was methanol-water(60:40, V/V) and detection wavelength was UV-245 nm. The minimum amounts of detection were 0.4 ng for diuron and 0.5 ng for chlortoluron. Recoveries were 87.34%-87.64% for diuron and 88.78%-91.94% for chlortoluron.
Han
H
H
Shandong Agricultural University, Tai'an, 271018.
Li
J
J
Cao
S
S
Huang
H
H
D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D004237:Diuron / Q000032:analysis*; D006540:Herbicides / Q000032:analysis; D008460:Meat / Q000032:analysis*; D008461:Meat Products / Q000032:analysis*; D010573:Pesticide Residues / Q000032:analysis*
D006540:Herbicides; D010573:Pesticide Residues; D004237:Diuron