[Sequential isolation of superoxide dismutase and ajmaline from a tissue culture of Rauwolfia serpentina Benth].
Two biologically active compounds, the enzyme superoxide dismutase (EC 1.15.1.1) and the antiarrhythmic indole alkaloid ajmaline, were isolated from a callus culture of Rauwolfia serpentina Benth. Sequential isolation of biologically active compounds by metal-chelate affinity chromatography followed by azoadsorbent affinity chromatography allowed us to obtain highly purified products. The yields of superoxide dismutase and ajmaline were 180 mg/kg biomass and 16.5 g/kg dry weight, respectively.
Kirillova
N V
NV
St. Petersburg State Chemico-Pharmaceutical Academy, St. Petersburg, 197376 Russia.
Smirnova
M G
MG
Komov
V P
VP
D000404:Ajmaline / Q000302:isolation & purification*; D000889:Anti-Arrhythmia Agents / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D010946:Plants, Medicinal; D011926:Rauwolfia / Q000201:enzymology / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000302:isolation & purification*
D000889:Anti-Arrhythmia Agents; D000404:Ajmaline; D013482:Superoxide Dismutase