Activation of the redox-regulated molecular chaperone Hsp33--a two-step mechanism.
BACKGROUND
Hsp33 is a novel redox-regulated molecular chaperone. Hsp33 is present in the reducing environment of the cytosol and is, under normal conditions, inactive. The four highly conserved cysteines found in Hsp33 constitute a novel zinc binding motif. Upon exposure to oxidative stress, Hsp33's chaperone activity is turned on. This activation process is initiated by the formation of two intramolecular disulfide bonds. Recently, the 2.2 A crystal structure of Hsp33 has been solved, revealing that Hsp33 is present as a dimer in the structure (Vijayalakshmi et al., this issue, 367-375 [1]).


RESULTS
We show here that oxidized, highly active Hsp33 is a dimer in solution. In contrast, reduced and inactive Hsp33 is monomeric. The incubation of reduced Hsp33 in H(2)O(2) leads to the simultaneous formation of two intramolecular disulfide bonds and the concomitant release of zinc. This concentration-independent step is followed by a concentration-dependent association reaction. The dimerization of Hsp33 requires highly temperature-sensitive structural rearrangements. This allows Hsp33's activation process to be greatly accelerated at heat shock temperatures.


CONCLUSIONS
The regulation of Hsp33's chaperone function is highly sophisticated. On a transcriptional level, Hsp33 is under heat shock control. This increases the concentration of Hsp33 under heat and oxidative stress, a process that favors dimerization, a critical step in Hsp33's activation reaction. On a posttranslational level, Hsp33 is redox regulated. Dimerization of disulfide-bonded Hsp33 monomers leads to the formation of two extended, putative substrate binding sites. These sites might explain Hsp33's high and promiscuous affinity for unstructured protein folding intermediates.
Graumann
J
J
Department of Biology, University of Michigan, 48109, Ann Arbor, MI, USA.
Lilie
H
H
Tang
X
X
Tucker
K A
KA
Hoffmann
J H
JH
Vijayalakshmi
J
J
Saper
M
M
Bardwell
J C
JC
Jakob
U
U
D001426:Bacterial Proteins; D019281:Dimerization; D004220:Disulfides; D004789:Enzyme Activation; D006360:Heat-Shock Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D018832:Molecular Chaperones / Q000737:chemistry / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D016296:Mutagenesis; D010084:Oxidation-Reduction; D011499:Protein Processing, Post-Translational; D017434:Protein Structure, Tertiary; D013696:Temperature; D015032:Zinc / Q000378:metabolism
D001426:Bacterial Proteins; D004220:Disulfides; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; D015032:Zinc