title: A 'cassette' RNase: site-selective cleavage of RNA by RNase S equipped with RNA-recognition segment.
abstract: RNase S is a unique protein comprising the non-covalent association of two components, the S-peptide and the S-protein. An RNA-recognition segment derived from the human immunodeficiency virus (HIV)-1 Rev protein was conjugated with the S-peptide to form a complex with the S-protein. The resulting RNase S bearing the RNA-recognition segment preferentially hydrolyzed a single position of the RNA stem-loop derived from the specific binding site for the Rev protein.
authors.affiliation: Institute for Chemical Research, Kyoto University, Uji, Japan. futaki@scl.kyoto-u.ac.jp
authors.forename: S
authors.initials: S
authors.lastname: Futaki
authors.forename: M
authors.initials: M
authors.lastname: Araki
authors.forename: T
authors.initials: T
authors.lastname: Kiwada
authors.forename: I
authors.initials: I
authors.lastname: Nakase
authors.forename: Y
authors.initials: Y
authors.lastname: Sugiura
mesh_terms: D000595:Amino Acid Sequence; D054303:HIV Reverse Transcriptase / Q000737:chemistry; D015497:HIV-1 / Q000737:chemistry; D006868:Hydrolysis; D008958:Models, Molecular; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000187:drug effects*; D012260:Ribonucleases / Q000494:pharmacology*; D013379:Substrate Specificity
chemical_list: D012367:RNA, Viral; D054303:HIV Reverse Transcriptase; D012260:Ribonucleases; C022542:ribonuclease S