Kanamycin susceptibility testing of Mycobacterium tuberculosis using Mycobacterium Growth Indicator Tube and a colorimetric method.
Two novel systems were evaluated for performing indirect kanamycin susceptibility tests on 72 strains of Mycobacterium tuberculosis. The microplate Alamar blue colorimetric method (breakpoint, 2.5 microg/ml) and the Mycobacterium Growth Indicator Tube (MGIT) system (breakpoint, 5.0 microg/ml) both produced 98.6% agreement when compared with the conventional proportion method performed on 7H10 agar using 5.0 microg of kanamycin/ml. Both systems provided results within an average of 1 week.
Bastian
I
I
Mycobacteriology Unit, Institute of Tropical Medicine, Antwerp, Belgium. ivan.bastien@imvs.sa.gov.au
Rigouts
L
L
Palomino
J C
JC
Portaels
F
F
D000995:Antitubercular Agents / Q000494:pharmacology*; D003124:Colorimetry; D004396:Coloring Agents; D004352:Drug Resistance, Microbial; D007612:Kanamycin / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000254:growth & development; D010078:Oxazines; D014966:Xanthenes
D000995:Antitubercular Agents; D004396:Coloring Agents; D010078:Oxazines; D014966:Xanthenes; C005843:resazurin; D007612:Kanamycin