title: Intravenous endotoxin triggers pulmonary vasoconstriction and pulmonary hypertension in broiler chickens.
abstract: Bacterial endotoxins stimulate endothelin-mediated, thromboxane-dependent increases in pulmonary vascular resistance in mammals, and thromboxane has been shown to cause an immediate but transient pulmonary vasoconstriction in broiler chickens. In the present study, i.v. injections of 1 mg endotoxin into anesthetized male broilers caused a pulmonary vasoconstrictive response that was delayed in onset by 15 min and that elevated the pulmonary arterial pressure by 10 mm Hg within 25 min postinjection. Thereafter, pulmonary hemodynamic variables gradually (> or = 15 min) returned toward pre-injection levels, and supplemental injections of 4 mg endotoxin during this recovery period failed to reinitiate pulmonary hypertension. In contrast, injecting the thromboxane A2 mimetic U44069 during the endotoxin recovery period triggered pulmonary vasoconstriction and pulmonary hypertension similar in magnitude to the responses triggered by U44069 before endotoxin had been administered. The time course and magnitude of the pulmonary hemodynamic responses to endotoxin were highly variable among individual broilers, whereas the individual responses to U44069 were more consistent. Unanesthetized broilers resembled anesthetized broilers in the time course, magnitude, and variability of their pulmonary hemodynamic responses to endotoxin. Overall, these observations are consistent with the hypothesis that endotoxin initiates a biochemical cascade, culminating in the delayed onset of pulmonary vasoconstriction and pulmonary hypertension within 20 min postinjection. Subsequently, the pulmonary vasculature remains responsive to large bolus injections of exogenous thromboxane mimetic; however depletion of endogenous vasoconstrictive components of the endotoxin-mediated cascade, a compensatory increase in endogenous vasodilators, or the induction of a transient cellular tolerance to endotoxin prevented fourfold higher doses of endotoxin from reversing the return toward a normal pulmonary vascular tone. Individual differences among broilers in their susceptibility to pulmonary hypertension syndrome (ascites) may be related to innate or acquired variability in their pulmonary vascular responsiveness to vasoactive mediators.
authors.affiliation: Department of Poultry Science, University of Arkansas, Fayetteville 72701, USA. rwideman@mail.uark.edu
authors.forename: R F
authors.initials: RF
authors.lastname: Wideman
authors.forename: G F
authors.initials: GF
authors.lastname: Erf
authors.forename: M E
authors.initials: ME
authors.lastname: Chapman
mesh_terms: D000818:Animals; D002645:Chickens / Q000502:physiology*; D004731:Endotoxins / Q000008:administration & dosage*; D006976:Hypertension, Pulmonary / Q000209:etiology / Q000662:veterinary*; D007275:Injections, Intravenous; D008297:Male; D011201:Poultry Diseases / Q000209:etiology; D011450:Prostaglandin Endoperoxides, Synthetic / Q000494:pharmacology*; D011652:Pulmonary Circulation / Q000187:drug effects; D013928:Thromboxane A2 / Q000494:pharmacology; D014655:Vascular Resistance; D014661:Vasoconstriction / Q000187:drug effects*
chemical_list: D004731:Endotoxins; D011450:Prostaglandin Endoperoxides, Synthetic; C028429:U 44069; D013928:Thromboxane A2