Surface modification of stainless steel by grafting of poly(ethylene glycol) for reduction in protein adsorption.
The surface of stainless steel was first modified by the silane coupling agent (SCA), (3-mercaptopropyl)trimethoxysilane. The silanized stainless-steel surface (SCA-SS surface) was subsequently activated by argon plasma and then subjected to UV-induced graft polymerization of poly(ethylene glycol)methacrylate (PEGMA). The chemical structures and composition of the pristine, silane-treated, plasma-treated and PEGMA graft-polymerized stainless-steel coupon surfaces were characterized by X-ray photoelectron spectroscopy (XPS) and attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy. The graft polymerization of PEGMA onto the plasma-pretreated SCA-SS surface was studied with different argon plasma pretreatment time, macromonomer concentration, and UV graft polymerization time. In general, a brief plasma pretreatment, high PEGMA concentration, and long UV graft polymerization time readily resulted in a high graft concentration. The PEGMA graft-polymerized stainless-steel coupon (PEGMA-g-SCA-SS) with a high graft concentration, and thus a high PEG content, was found to be very effective in preventing bovine serum albumin and gamma-globulin adsorption.
Zhang
F
F
Department of Chemical Engineering, National University of Singapore, Crescent.
Kang
E T
ET
Neoh
K G
KG
Wang
P
P
Tan
K L
KL
D000327:Adsorption; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D005026:Ethylene Glycols / Q000737:chemistry*; D008422:Materials Testing / Q000379:methods; D017646:Organosilicon Compounds; D011092:Polyethylene Glycols / Q000737:chemistry*; D011109:Polymethacrylic Acids / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry; D012821:Silanes / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013193:Stainless Steel / Q000737:chemistry*; D013499:Surface Properties
D020099:Coated Materials, Biocompatible; D005026:Ethylene Glycols; D017646:Organosilicon Compounds; D011109:Polymethacrylic Acids; D011506:Proteins; D012821:Silanes; D013193:Stainless Steel; C024546:diethylene glycol dimethacrylate polymer; D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols; C102833:(3-mercaptopropyl)trimethoxysilane