The suppression effect of light rare earth elements on proliferation of two cancer cell lines.
To study the suppression effect of light rare earth elements (RE) on proliferation of two cancer cell lines. Two cancer cell lines PAMC82 and K562 were used to examine their colony-forming ability in soft agar, microtubule structure, calmodulin levels and regulation of some gene expressions by Northern blot analysis with and without treatment by RE. The results showed that on soft agar culture the colony-forming ability of human gastric cancer cell line PAMC82 treated by RE chloride decreased and the PAMC82 cell microtubule abnormal structure became normal. The calmodulin (CaM) levels decreased in human leukemia cells (K562) treated with cerium chloride and neodymium chloride. The Northern blot analysis revealed marked up-regulation of p53, p16(MTS1), p21 (WAF1) gene expressions in PAMC82 cells treated with lanthanum chloride and cerium chloride, as compared to control PAMC82 cells. The light rare earth elements studied have certain suppression effects on proliferation of cancer cells. This effect might be related to the decrease of calmodulin and up-regulation of some gene expressions in cancer cells.
Ji
Y J
YJ
Beijing Institute of Labor Hygiene and Occupational Diseases, Beijing 100020, China.
Xiao
B
B
Wang
Z H
ZH
Cui
M Z
MZ
Lu
Y Y
YY
D002147:Calmodulin / Q000032:analysis; D002455:Cell Division / Q000187:drug effects; D003114:Colony-Forming Units Assay; D016147:Genes, Tumor Suppressor; D006801:Humans; D007938:Leukemia; D008674:Metals, Rare Earth / Q000494:pharmacology*; D008870:Microtubules / Q000648:ultrastructure; D013274:Stomach Neoplasms; D014407:Tumor Cells, Cultured / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure
D002147:Calmodulin; D008674:Metals, Rare Earth