title: Three days of incubation may be sufficient for routine blood cultures with BacT/Alert FAN blood culture bottles.
abstract: BacT/Alert FAN blood culture bottles have been shown to enhance the recovery of bacteria and yeast from blood compared with standard BacT/Alert bottles. It is well established that standard BacT/Alert blood culture bottles require no more than 5 days of incubation for the detection of routine bacteria and yeast. It is less clear, however, whether FAN bottles also routinely require 5 days of incubation. To address this question, we recently reviewed the results of 17,887 blood culture sets collected in FAN blood culture bottles at Geisinger Medical Center. Of these cultures, 1,780 were positive for bacteria or yeast, yielding a total of 1,242 clinically significant isolates. The numbers of isolates recovered on days 1, 2, 3, 4, and 5 were as follows: (values in parentheses are percentages of total significant isolates): 877 (71%), 269 (22%), 65 (5%), 18 (1%) and, 13 (1%), respectively. In total, 97.5% of all clinically significant isolates were detected in the first 3 days of incubation. Of the 31 significant isolates detected on day 4 or 5 of incubation, 17 were detected in concurrent blood cultures within the first 3 days of incubation. Chart reviews were conducted for the 13 patients with the remaining 14 isolates detected on day 4 or 5 to determine whether therapy was changed due to this blood culture result. Therapy was changed for only 1 patient. These results suggest that it may not be necessary to routinely incubate FAN blood culture bottles for more than 3 days.
authors.affiliation: Division of Laboratory Medicine, Geisinger Medical Center, Danville, PA 17822-0131, USA. pbourbeau@geisinger.edu
authors.forename: P P
authors.initials: PP
authors.lastname: Bourbeau
authors.forename: J K
authors.initials: JK
authors.lastname: Pohlman
mesh_terms: D000332:Aerobiosis; D000693:Anaerobiosis; D001419:Bacteria / Q000302:isolation & purification*; D001424:Bacterial Infections / Q000382:microbiology; D001769:Blood / Q000382:microbiology*; D003470:Culture Media; D006801:Humans; D009181:Mycoses / Q000382:microbiology; D011933:Reagent Kits, Diagnostic; D013997:Time Factors; D015003:Yeasts / Q000302:isolation & purification*
chemical_list: D003470:Culture Media; D011933:Reagent Kits, Diagnostic