title: Crystallization and preliminary crystallographic data of fructose-1,6-bisphosphatase from human muscle.
abstract: The enzyme human muscle fructose-1,6-bisphosphatase, which plays a critical role in gluconeogenesis, has been crystallized in the presence of 2-propanol, polyethylene glycol and magnesium chloride at pH 7.5. The space group was determined to be P4(2)2(1)2, with unit-cell parameters a = b = 73.57, c = 146.50 A, alpha = beta = lambda = 90 degrees and one subunit in the asymmetric unit. A 99.6% complete data set to 2.04 A has been collected at the National Synchrotron Light Source.
authors.affiliation: The Laboratory of Molecular Endocrinology, CHUL Research Center and Laval University, Quebec G1V 4G2, Canada.
authors.forename: D W
authors.initials: DW
authors.lastname: Zhu
authors.forename: G J
authors.initials: GJ
authors.lastname: Xu
authors.forename: P H
authors.initials: PH
authors.lastname: Rehse
authors.forename: R
authors.initials: R
authors.lastname: Shi
authors.forename: F K
authors.initials: FK
authors.lastname: Zhao
authors.forename: S X
authors.initials: SX
authors.lastname: Lin
mesh_terms: D019840:2-Propanol / Q000737:chemistry; D003460:Crystallization; D018360:Crystallography, X-Ray; D006597:Fructose-Bisphosphatase / Q000737:chemistry*; D006801:Humans; D008274:Magnesium / Q000737:chemistry; D009132:Muscles / Q000201:enzymology*; D011092:Polyethylene Glycols / Q000737:chemistry; D011487:Protein Conformation
chemical_list: D011092:Polyethylene Glycols; D006597:Fructose-Bisphosphatase; D008274:Magnesium; D019840:2-Propanol