title: Genetic and molecular features of Su(P), a gene that interacts with ref(2)P in male fertility of Drosophila melanogaster.
abstract: The ref(2)P gene is involved in the control of sigma rhabdovirus multiplication in Drosophila melanogaster. ref(2)P activity is also necessary for male fertility. However, in one-third of laboratory strains tested, males that lacked ref(2)P activity were fertile. In all such strains studied, the male sterility phenotype was abolished due to the presence of a particular allele at the Su(P) locus, at 73B1-2. These spontaneous suppressor alleles were dominant. We were able to induce dominant suppressor alleles at the Su(P) locus by X-ray mutagenesis and hybrid dysgenesis, suggesting that null alleles of Su(P) confer the dominant suppressor phenotype. The Su(P) gene was cloned by P element tagging. The P element-tagged alleles identified a Su(P) transcript as a 1.4-kb mRNA produced in the soma of both males and females, which is also abundant in ovaries.
authors.affiliation: Centre de Génétique Moléculaire, CNRS, Gif-sur-Yvette, France.
authors.forename: A
authors.initials: A
authors.lastname: Bichon
authors.forename: N
authors.initials: N
authors.lastname: Boukhatem
authors.forename: P
authors.initials: P
authors.lastname: Gay
authors.forename: P
authors.initials: P
authors.lastname: Dru
authors.forename: H
authors.initials: H
authors.lastname: Terzian
authors.forename: A M
authors.initials: AM
authors.lastname: Petitjean
authors.forename: F
authors.initials: F
authors.lastname: Lemeunier
authors.forename: D
authors.initials: D
authors.lastname: Contamine
mesh_terms: D000483:Alleles; D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000235:genetics*; D005260:Female; D005298:Fertility / Q000502:physiology*; D017344:Genes, Insect / Q000502:physiology*; D019476:Insect Proteins / Q000235:genetics*; D008297:Male; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics*; D009687:Nuclear Proteins; D012313:RNA; D050825:Synaptosomal-Associated Protein 25
chemical_list: D004251:DNA Transposable Elements; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D019476:Insect Proteins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; C428923:Su(P) protein, Drosophila; D050825:Synaptosomal-Associated Protein 25; C080596:ref(2)P protein, Drosophila; D012313:RNA