title: Analysis of Asn-linked glycans from vegetable foodstuffs: widespread occurrence of Lewis a, core alpha1,3-linked fucose and xylose substitutions.
abstract: The N-glycans from 27 "plant" foodstuffs, including one from a gymnospermic plant and one from a fungus, were prepared by a new procedure and examined by means of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). For several samples, glycan structures were additionally investigated by size-fractionation and reverse-phase high-performance liquid chromatography in conjunction with exoglycosidase digests and finally also (1)H-nuclear magnetic resonance spectroscopy. The glycans found ranged from the typical vacuolar "horseradish peroxidase" type and oligomannose to complex Le(a)-carrying structures. Though the common mushroom exclusively contained N-glycans of the oligomannosidic type, all plant foods contained mixtures of the above-mentioned types. Apple, asparagus, avocado, banana, carrot, celery, hazelnut, kiwi, onion, orange, pear, pignoli, strawberry, and walnut were particularly rich in Le(a)-carrying N-glycans. Although traces of Le(a)-containing structures were also present in almond, pistachio, potato, and tomato, no such glycans could be found in cauliflower. Coconut exhibited almost exclusively N-glycans containing only xylose but no fucose. Oligomannosidic N-glycans dominated in buckwheat and especially in the legume seeds mung bean, pea, peanut, and soybean. Papaya presented a unique set of hybrid type structures partially containing the Le(a) determinant. These results are not only compatible with the hypothesis that the carbohydrate structures are another potential source of immunological cross-reaction between different plant allergens, but they also demonstrate that the Le(a)-type structure is very widespread among plants.
authors.affiliation: Institut für Chemie der Universität für Bodenkultur, Muthgasse 18, A-1190 Wien, Austria.
authors.forename: I B
authors.initials: IB
authors.lastname: Wilson
authors.forename: R
authors.initials: R
authors.lastname: Zeleny
authors.forename: D
authors.initials: D
authors.lastname: Kolarich
authors.forename: E
authors.initials: E
authors.lastname: Staudacher
authors.forename: C J
authors.initials: CJ
authors.lastname: Stroop
authors.forename: J P
authors.initials: JP
authors.lastname: Kamerling
authors.forename: F
authors.initials: F
authors.lastname: Altmann
mesh_terms: D001216:Asparagine / Q000378:metabolism*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D000939:Epitopes / Q000032:analysis / Q000737:chemistry / Q000276:immunology; D005512:Food Hypersensitivity; D005638:Fruit / Q000737:chemistry; D005643:Fucose / Q000031:analogs & derivatives / Q000032:analysis* / Q000737:chemistry; D005658:Fungi / Q000737:chemistry; D005690:Galactose / Q000032:analysis; D006026:Glycoside Hydrolases / Q000378:metabolism; D007983:Lewis Blood Group Antigens / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009844:Oligosaccharides / Q000032:analysis / Q000737:chemistry / Q000276:immunology; D011134:Polysaccharides / Q000032:analysis* / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D012639:Seeds / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014675:Vegetables / Q000737:chemistry* / Q000276:immunology; D014994:Xylose / Q000032:analysis*
chemical_list: D000939:Epitopes; D007983:Lewis Blood Group Antigens; D009844:Oligosaccharides; D011134:Polysaccharides; C028569:oligomannoside; D005643:Fucose; D001216:Asparagine; D014994:Xylose; D006026:Glycoside Hydrolases; D005690:Galactose