Phospholipase D1: a key factor for the exocytotic machinery in neuroendocrine cells.
Phospholipase D (PLD) has been proposed to mediate cytoskeletal remodeling and vesicular trafficking along the secretory pathway. We recently described the activation of an ADP ribosylation factor-regulated PLD at the plasma membrane of chromaffin cells undergoing secretagogue-stimulated exocytosis. We show here that the isoform involved is PLD1b, and, using a real-time assay for individual cells, that PLD activation and exocytosis are closely correlated. Moreover, overexpressed PLD1, but not PLD2, increases stimulated exocytosis in a phosphatidylinositol 4,5-bisphosphate-dependent manner, whereas catalytically inactive PLD1 inhibits it. These results provide the first direct evidence that PLD1 is an important component of the exocytotic machinery in neuroendocrine cells.
Vitale
N
N
CNRS-UPR 2356 Neurotransmission et Sécrétion Neuroendocrine, 5 rue Blaise Pascal, 67084 Strasbourg, France.
Caumont
A S
AS
Chasserot-Golaz
S
S
Du
G
G
Wu
S
S
Sciorra
V A
VA
Morris
A J
AJ
Frohman
M A
MA
Bader
M F
MF
D000199:Actins / Q000378:metabolism; D000818:Animals; D002384:Catalysis; D002417:Cattle; D002478:Cells, Cultured; D019439:Chromaffin Cells / Q000166:cytology / Q000201:enzymology* / Q000502:physiology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005089:Exocytosis / Q000502:physiology*; D007424:Intracellular Fluid / Q000201:enzymology; D007527:Isoenzymes / Q000037:antagonists & inhibitors / Q000378:metabolism; D009490:Neurosecretory Systems / Q000166:cytology; D016716:PC12 Cells; D019269:Phosphatidylinositol 4,5-Diphosphate / Q000378:metabolism; D010739:Phospholipase D / Q000037:antagonists & inhibitors / Q000378:metabolism*; D011493:Protein Kinase C / Q000378:metabolism; D051381:Rats; D013110:Sphingosine / Q000031:analogs & derivatives / Q000494:pharmacology
D000199:Actins; D004791:Enzyme Inhibitors; D007527:Isoenzymes; C064769:N-acetylsphingosine; D019269:Phosphatidylinositol 4,5-Diphosphate; D011493:Protein Kinase C; D010739:Phospholipase D; C106801:phospholipase D1; D013110:Sphingosine