A single bout of eccentric exercise increases HSP27 and HSC/HSP70 in human skeletal muscle.
Changes in heat shock proteins (HSPs), HSP27 and HSC/HSP70 were characterized in human biceps brachii muscle following damaging high-force eccentric exercise. Male and female volunteers performed a maximal eccentric resistance exercise with the elbow flexor muscles of the non-dominant arm known to be sufficient to cause substantial muscle damage. Protein extracts of biopsy tissue samples taken 48 h post-exercise were immunoblotted for HSC/HSP70 and HSP27. Densitometric analysis demonstrated that these proteins increased significantly (P < 0.01) in the damaged biceps brachii relative to the control arm. The HSC/HSP70 increased 1064% in the exercised sample while HSP27 increased by 234%. Although the literature reports a muscular heat shock response following aerobic, oxidative exercise, this is the first documentation of increases in protein expression of both HSC/HSP70 and HSP27 in human skeletal muscle in response to a single bout of resistance exercise.
Thompson
H S
HS
Department of Biological Sciences, Smith College, Northampton, MA, USA.
Scordilis
S P
SP
Clarkson
P M
PM
Lohrer
W A
WA
D000199:Actins / Q000378:metabolism; D000328:Adult; D015444:Exercise / Q000502:physiology*; D005080:Exercise Test; D005260:Female; D055551:HSP27 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D006360:Heat-Shock Proteins; D006801:Humans; D015151:Immunoblotting; D008297:Male; D018832:Molecular Chaperones; D018482:Muscle, Skeletal / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000378:metabolism*
D000199:Actins; D055551:HSP27 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; C057725:HSPB1 protein, human; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; D009363:Neoplasm Proteins