Molecular cloning of the anther-preferential nonspecific lipid transfer protein cDNA in hot pepper by mRNA differential display.
To better understand the molecular control of anther development, an anther-preferential mRNA was isolated from hot pepper (Capsicum annuum) using mRNA differentially display. Using the displayed fragment as a probe, a full-length cDNA named CaLTP was isolated. A nucleotide sequence analysis of CaLTP revealed that the clone contains an open reading frame of 123 amino acids, which exhibits a 60-23% identity with nonspecific lipid transfer proteins (nsLTP). Northern and RT-PCR analysis of the clone confirmed that CaLTP mRNA was predominant to anther tissues. The basal expression level in the leaves was slightly induced only by abscisic acid (ABA) treatment. Southern analysis reveals that CaLTP is present as a single-copy gene in hot pepper genome. We hypothesize that CaLTP might have an important role in protecting the reproductive tissues from environmental stresses.
Hong
S T
ST
Department of Life Science, Sogang University, Seoul, Korea.
Lee
M Y
MY
Kim
S R
SR
D000595:Amino Acid Sequence; D052179:Antigens, Plant; D001483:Base Sequence; D002212:Capsicum / Q000033:anatomy & histology / Q000737:chemistry / Q000235:genetics*; D002352:Carrier Proteins / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D018744:DNA, Plant / Q000378:metabolism*; D020869:Gene Expression Profiling; D017930:Genes, Reporter; D015151:Immunoblotting; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D018514:Plant Structures / Q000502:physiology*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment
D052179:Antigens, Plant; D002352:Carrier Proteins; D018744:DNA, Plant; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; C511920:lipid transfer proteins, plant