[Effect of liposome-C-erbB2 antisense oligodeoxynucleotides on human ovarian cancer cells].
OBJECTIVE
To explore the effects of liposome-C-erbB2 antisense phosphorothioate oligo deoxynucleotides(S-ODNs) on C-erbB2 protooncogene expression and cell proliferation in human ovarian cancer cells.


METHODS
The effects on C-erbB2 protooncogene expression, cell proliferation and cell cycle in human ovarian cancer cells were studied by flow cytometry and 3H-thymidine incorporation.


RESULTS
Liposome-C-erbB2 S-ODNs could reduce C-erbB2 expression and inhibit cell proliferation in human ovarian cancer cells; the effectiveness of liposome-C-erbB2 S-ODNs on the expression of C-erbB2 was much higher than that of C-erbB2 S-ODNs, about 40 times.


CONCLUSIONS
The data in this study suggest that antisense therapy is an useful method of gene therapy in ovarian cancer. The effectiveness of C-erbB2 S-ODNs could be greatly increased by C-erbB2 S-ODNs encapsulated in liposomes.
Shen
M
M
Gynecology and Obstetrics Hospital, Shanghai Medical University, Shanghai 200011.
Feng
Y
Y
Ge
B
B
D018283:Cystadenocarcinoma, Papillary / Q000473:pathology*; D005260:Female; D018734:Genes, erbB-2; D006801:Humans; D008081:Liposomes; D020319:Oligodeoxyribonucleotides, Antisense / Q000494:pharmacology*; D010051:Ovarian Neoplasms / Q000473:pathology*; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000096:biosynthesis*; D014407:Tumor Cells, Cultured
D008081:Liposomes; D020319:Oligodeoxyribonucleotides, Antisense; D018719:Erb-b2 Receptor Tyrosine Kinases