Regulation of glutamine transport by the type I insulin-like growth factor receptor in a human neuroblastoma cell line.
We examined the effects of the monoclonal antibody against the type I insulin-like growth factor receptor (IGF-IR), alphaIR3, on cell growth and membrane glutamine (Gln) transport in a human neuroblastoma cell line, SK-N-SH. In the presence of alphaIR3 (2 microg/ml), the cell proliferation rate was significantly attenuated. Gln transport was decreased in the alphaIR3-treated group with the mechanism of decreasing maximum transport velocity without affecting transport affinity. The addition of alphaIR3 significantly decreased DNA and protein biosynthesis. Our results support the concept that the activation of IGF-IR partially mediates neuroblastoma cell proliferation by regulating membrane Gln transport.
Wang
H S
HS
Department of Pediatric Surgery, Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita, 565-0871, Osaka, Japan.
Wasa
M
M
Okada
A
A
D017209:Apoptosis; D002455:Cell Division; D002462:Cell Membrane / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005973:Glutamine / Q000378:metabolism*; D006801:Humans; D007930:Leucine / Q000378:metabolism; D009447:Neuroblastoma / Q000378:metabolism*; D021381:Protein Transport; D017526:Receptor, IGF Type 1 / Q000378:metabolism*; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors; D014407:Tumor Cells, Cultured
D005973:Glutamine; D017526:Receptor, IGF Type 1; D007930:Leucine; D013936:Thymidine