title: Pulmonary edema fluid from patients with acute lung injury augments in vitro alveolar epithelial repair by an IL-1beta-dependent mechanism.
abstract: Efficient alveolar epithelial repair is crucial for the restoration of the injured alveolar epithelial barrier in patients with acute lung injury (ALI) and the acute respiratory distress syndrome (ARDS). We hypothesized that pulmonary edema fluid from patients with ALI /ARDS would inhibit alveolar epithelial repair as measured in an in vitro epithelial wound-repair model using the human alveolar epithelial-like cell line A549. In contrast to our initial hypothesis, pulmonary edema fluid from patients with ALI/ARDS increased alveolar epithelial repair by 33 +/- 3% compared with pooled plasma from healthy donors (p < 0.01). By contrast, the plasma and the pulmonary edema fluid from patients with hydrostatic pulmonary edema, and the plasma from patients with ALI/ARDS had similar effects on epithelial repair as pooled plasma from healthy donors. Inhibition of interleukin-1beta (IL-1beta) activity by IL-1 receptor antagonist reduced alveolar epithelial repair induced by ALI/ARDS edema fluid by 46 +/- 4% (p < 0.001), indicating that IL-1beta contributed significantly to the increased epithelial repair. In summary, pulmonary edema fluid collected early in the course of ALI/ARDS increased alveolar epithelial repair in vitro by an IL-1beta-dependent mechanism. These data demonstrate a novel role for IL-1beta in patients with ALI/ARDS, indicating that IL-1beta may promote repair of the injured alveolar epithelium.
authors.affiliation: Cardiovascular Research Institute, University of California San Francisco, San Francisco, CA, USA. Thomas.Geiser@insel.ch
authors.forename: T
authors.initials: T
authors.lastname: Geiser
authors.forename: K
authors.initials: K
authors.lastname: Atabai
authors.forename: P H
authors.initials: PH
authors.lastname: Jarreau
authors.forename: L B
authors.initials: LB
authors.lastname: Ware
authors.forename: J
authors.initials: J
authors.lastname: Pugin
authors.forename: M A
authors.initials: MA
authors.lastname: Matthay
mesh_terms: D000328:Adult; D000368:Aged; D000704:Analysis of Variance; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology*; D016022:Case-Control Studies; D002460:Cell Line; D004353:Drug Evaluation, Preclinical; D004848:Epithelium / Q000276:immunology*; D006801:Humans; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1 / Q000037:antagonists & inhibitors / Q000276:immunology* / Q000627:therapeutic use*; D008875:Middle Aged; D010949:Plasma / Q000276:immunology; D011650:Pulmonary Alveoli / Q000166:cytology* / Q000276:immunology*; D011654:Pulmonary Edema / Q000150:complications / Q000473:pathology* / Q000628:therapy*; D012038:Regeneration / Q000276:immunology*; D012128:Respiratory Distress Syndrome / Q000150:complications / Q000473:pathology* / Q000628:therapy*; D012795:Sialoglycoproteins / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology / Q000627:therapeutic use
chemical_list: C507763:IL1RN protein, human; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1; D012795:Sialoglycoproteins; D014409:Tumor Necrosis Factor-alpha