Preliminary X-ray crystallographic analysis of a Ca2+-binding protein human S100A1.
S100A1, a Ca(2+)-binding protein from the S100 protein family, has been crystallized by the vapour-diffusion method using polyethylene glycol 4000 as the precipitant at pH 8.5. The crystal belongs to space group P6(3). The unit-cell parameters are a = b = 57.3, c = 104.7 A. There appear to be two S100A1 molecules in the asymmetric unit. The crystals were stable during exposure to X-rays and diffract to 2.6 A resolution in-house.
Wang
Z
Z
Laboratory of Structural Biology, School of Life Science and Engineering and Protein Science Laboratory of the MOE, Tsinghua University, Beijing 100084, People's Republic of China.
Zhang
H
H
Ding
Y
Y
Wang
G
G
Wang
X
X
Ye
S
S
Bartlam
M
M
Tang
H
H
Liu
Y
Y
Jiang
F
F
Barraclough
R
R
Rudland
P S
PS
Rao
Z
Z
D002135:Calcium-Binding Proteins / Q000737:chemistry* / Q000302:isolation & purification; D003460:Crystallization; D018360:Crystallography, X-Ray; D006801:Humans; D011487:Protein Conformation; D009418:S100 Proteins; D013223:Statistics as Topic
D002135:Calcium-Binding Proteins; D009418:S100 Proteins; C110294:S100A1 protein