Growth-rate regulation of the Bacillus subtilis accBC operon encoding subunits of acetyl-CoA carboxylase, the first enzyme of fatty acid synthesis.
Acetyl-CoA carboxylase catalyses the synthesis of malonyl-CoA, the first intermediate in fatty acid (hence phospholipid) synthesis. The accBC operon from Bacillus subtilis codes for two subunits of acetyl-CoA carboxylase, biotin carboxyl-carrier and biotin carboxylase. In this work, the 5'-end of the accBC mRNA was determined by primer-extension, and transcriptional fusion analysis of the accBC promoter was carried out under a variety of growth conditions. A direct correlation between the levels of transcription of the accBC genes and the rate of cellular growth is reported. Consistent results were also obtained in nutritional upshift and downshift experiments.
Marini
P E
PE
Instituto de Biología Molecular y Celular de Rosario (IBR) and Departamento de Microbiología, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 531, 2000-Rosario, Argentina.
Perez
C A
CA
de Mendoza
D
D
D000103:Acetyl-CoA Carboxylase / Q000235:genetics*; D001412:Bacillus subtilis / Q000235:genetics / Q000254:growth & development*; D005227:Fatty Acids / Q000096:biosynthesis; D009876:Operon; D021122:Protein Subunits; D014158:Transcription, Genetic
D005227:Fatty Acids; D021122:Protein Subunits; D000103:Acetyl-CoA Carboxylase