title: Glycosylation changes during differentiation of the murine uterine epithelium.
abstract: In mouse uterine luminal epithelium (LE) several terminal carbohydrate structures are regulated by ovarian steroids and show stage-specific expression during early pregnancy. We have demonstrated that expression of H-type-1 antigen (Fucalpha1-2Galbeta1-3GlcNAcbeta1-) is regulated by oestrogenic stimulation of alpha1-2fucosyltransferase (fut1) mRNA levels in LE. H-type-1 expression is high after ovulation but becomes negligible after implantation. In contrast, NeuNAcalpha2-3Galbeta1-, and specifically sialyl Le-x (NeuAcalpha2,3Galbeta1,4[Fucalpha1-3]GlcNAcbeta1-), is stimulated by progesterone. It is not expressed on LE after ovulation but is expressed maximally on apical LE at the time of and after implantation. However, mRNA levels for 4 out of 5 known Gal/GalNAc alpha2-3sialyltransferases appear not to change in LE during early pregnancy, suggesting an alternative level of control.
authors.affiliation: School of Biological Sciences, University of Manchester, 3.239 Stopford Building, Oxford Rd., Manchester M13 9PT, U.K. skimber@fs1.scg.man.ac.uk
authors.forename: S J
authors.initials: SJ
authors.lastname: Kimber
authors.forename: R E
authors.initials: RE
authors.lastname: Stones
authors.forename: S S
authors.initials: SS
authors.lastname: Sidhu
mesh_terms: D000818:Animals; D000943:Antigens, Differentiation / Q000378:metabolism; D050260:Carbohydrate Metabolism; D002454:Cell Differentiation; D010064:Embryo Implantation; D004717:Endometrium / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D004847:Epithelial Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D004967:Estrogens / Q000378:metabolism / Q000494:pharmacology; D005260:Female; D005647:Fucosyltransferases / Q000378:metabolism; D006031:Glycosylation / Q000187:drug effects; D008562:Membrane Glycoproteins / Q000378:metabolism*; D051379:Mice; D010052:Ovariectomy; D011247:Pregnancy; D012794:Sialic Acids / Q000378:metabolism; D012799:Sialyltransferases / Q000378:metabolism; D000097631:Galactoside 2-alpha-L-fucosyltransferase
chemical_list: D000943:Antigens, Differentiation; D004967:Estrogens; D005647:Fucosyltransferases; D008562:Membrane Glycoproteins; D012794:Sialic Acids; D012799:Sialyltransferases; D000097631:Galactoside 2-alpha-L-fucosyltransferase; C403410:H-type-1 antigen