[A study of Nexin 1 of skin and hair follicle during postnatal development period of rat].
OBJECTIVE
The changes of Nexin 1 mRNA of skin hair follicles of postnatal rats were investigated on tissue sections in this paper in order to get more information about the possible functions of Nexin 1 in regulating of hair growth and cycle.


METHODS
The distribution and quantity of Nexin 1 mRNA on the paraffin sections of skin and hair follicle of Wistar rats, aged from postnatal 1 day to 61 day, were investigated by radiolabel In Situ Hybridization technique.


RESULTS
When the rats were 4 days old, the signals of Nexin 1 mRNA in the follicular papillary cells were observed. The signals of Nexin 1 mRNA showed a regular changes in the hair cycles: the signal of Nexin 1 mRNA appeared in the early stage of anagen phase and became higher level gradually, the peak was in the middle-late(IV) stage of anagen and decreased in later anagen but it totally disappeared when the follicles entered into catagen and telegen phases of hair cycles. Nexin 1 message was only accumulated in follicular papillary cells but no signal of Nexin 1 mRNA was detected in any other skin and hair follicular cells, including dermal fibroblasts, mast cells, adipocytes, endothelial or muscle cells, keratinocytes of skin and in different layers of hair follicle were showing also negative.


CONCLUSIONS
These results suggested a possible role of Nexin 1 which was a potent serine-protease inhibitor and have been shown in many organs to play important roles in regulating cellular growth and differentiation in regulating hair follicular growth.
Xiang
M
M
Southwestern Hospital, Third Military Medical University, Chongqing 400038.
Yang
T
T
D016564:Amyloid beta-Protein Precursor; D000818:Animals; D000831:Animals, Newborn; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D006197:Hair / Q000254:growth & development; D018859:Hair Follicle / Q000378:metabolism*; D008297:Male; D058255:Protease Nexins; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D011956:Receptors, Cell Surface; D015842:Serine Proteinase Inhibitors / Q000096:biosynthesis / Q000235:genetics; D012867:Skin / Q000378:metabolism*
D016564:Amyloid beta-Protein Precursor; D002352:Carrier Proteins; D058255:Protease Nexins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D015842:Serine Proteinase Inhibitors