title: Copper(II)-lincomycin: complexation pattern and oxidative activity.
abstract: Coordination of Cu(II) to lincomycin was studied by potentiometry, UV-Vis, circular dichroism (CD), EPR, NMR, cyclic voltammetry (CV) and ESI-MS. Only mononuclear complexes of stoichiometries ranging from CuL to CuH(-3)L were found. In the main species present at neutral pH, CuH(-2)L, lincomycin bonds Cu(II) through both of its nitrogen donors, and a deprotonated oxygen donor at C4 of the sugar moiety. High pressure liquid chromatography (HPLC) of products of 2'-deoxyguanosine (dG) oxidation and agarose gel electrophoresis of plasmid DNA confirmed that lincomycin complexes effectively facilitate dG oxidation by H2O2, but are not able to cleave double-stranded plasmid DNA.
authors.affiliation: Faculty of Chemistry, University of Wrocław, F. Joliot-Curie, Poland. mjb@wchuwr.chem.uni.wroc.pl
authors.forename: M
authors.initials: M
authors.lastname: Jezowska-Bojczuk
authors.forename: W
authors.initials: W
authors.lastname: Lesniak
authors.forename: W
authors.initials: W
authors.lastname: Szczepanik
authors.forename: K
authors.initials: K
authors.lastname: Gatner
authors.forename: A
authors.initials: A
authors.lastname: Jezierski
authors.forename: M
authors.initials: M
authors.lastname: Smoluch
authors.forename: W
authors.initials: W
authors.lastname: Bal
mesh_terms: D000900:Anti-Bacterial Agents / Q000378:metabolism* / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D003300:Copper / Q000378:metabolism*; D003849:Deoxyguanosine / Q000378:metabolism; D004563:Electrochemistry; D006861:Hydrogen Peroxide / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008024:Ligands; D008034:Lincomycin / Q000378:metabolism* / Q000494:pharmacology; D015394:Molecular Structure; D010084:Oxidation-Reduction / Q000187:drug effects; D010957:Plasmids / Q000187:drug effects / Q000378:metabolism; D013057:Spectrum Analysis; D017186:Titrimetry
chemical_list: D000900:Anti-Bacterial Agents; D008024:Ligands; D003300:Copper; D006861:Hydrogen Peroxide; D008034:Lincomycin; D003849:Deoxyguanosine