Rabbit tissue distribution of 3H-ouabain by digital radioautography.
3H-ouabain is useful to evaluate the tissue localization of Na,K-ATPase. In this work we determined the distribution of 3H-ouabain in rabbit tissue by digital radioautography. Using this method, we were able to obtain a comparison of various organs in a relatively short time (6.5 days), while with traditional radioautography, only the kidney was detectable after seven months of film exposure. The kidney has the highest intensity (concentration of 3H-ouabain), followed by the heart, liver, muscle, lung, spleen and finally brain, which was almost undetectable. In kidney the activity was higher in the cortex, while the other tissues displayed a more uniform intensity, suggesting that Na,K-ATPase was evenly distributed.
Balzan
S
S
CNR Istituto di Fisiologia Clinica, Pisa, Italy. balzan@nsifc.ifc.pi.cnr.its
Di Luca
L
L
Taddei
L
L
Salvador
P
P
Montali
U
U
D000818:Animals; D001345:Autoradiography / Q000379:methods*; D004791:Enzyme Inhibitors / Q000378:metabolism; D007668:Kidney / Q000201:enzymology / Q000378:metabolism; D008099:Liver / Q000201:enzymology / Q000378:metabolism; D008297:Male; D009132:Muscles / Q000201:enzymology / Q000378:metabolism; D009206:Myocardium / Q000201:enzymology / Q000378:metabolism; D010042:Ouabain / Q000378:metabolism*; D011817:Rabbits; D011869:Radioligand Assay; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D013223:Statistics as Topic; D013997:Time Factors; D014018:Tissue Distribution; D014316:Tritium
D004791:Enzyme Inhibitors; D014316:Tritium; D010042:Ouabain; D000254:Sodium-Potassium-Exchanging ATPase