Cloning of dynein intermediate chain cDNA of the newt Cynops pyrrhogaster.
To isolate genes whose expression is up-regulated after initiation of meiosis, we employed an mRNA differential display method using RNA extracted from newt testis fragments in the spermatogonial and spermatocyte stages. We report here isolation of a spermatocyte stage-specific cDNA clone encoding a newt homologue of dynein intermediate chain (IC). The newt dynein IC cDNA was found to encode a polypeptide consisting of 694 amino acid residues with 66.8% and 45.8% amino acid sequence similarity to sea urchin dynein IC3 and Chlamydomonas IC69, respectively. The predicted protein contains five WD repeats and a novel repeated motif in the C-terminal region. Northern blot analysis revealed that newt dynein IC mRNA was expressed in the spermatocyte and round spermatid stages, suggesting that dynein IC plays a role in formation of flagella as well as in meiotic events.
Yamamoto
T
T
Department of Biological Science, Faculty of Science, Graduate School of Science and Technology, Kumamoto University, Japan. tybig@gpo.kumamoto-u.ac.jp
Matsuda
Y
Y
Hikino
T
T
Nakayama
Y
Y
Abé
S
S
D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004398:Dyneins; D008297:Male; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology; D020449:Repetitive Sequences, Amino Acid; D012455:Salamandridae / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D013090:Spermatocytes / Q000378:metabolism; D013091:Spermatogenesis / Q000235:genetics*; D013093:Spermatogonia / Q000378:metabolism; D013737:Testis / Q000378:metabolism / Q000502:physiology; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
D018076:DNA, Complementary; D010455:Peptides; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; D004398:Dyneins