Electrophoresis separation in open microchannels. A method for coupling electrophoresis with MALDI-MS.
The separation of biological mixtures in open micro-channels using electrophoresis with rapid and simple coupling to mass spectrometry is introduced. Rapid open-access channel electrophoresis employs microchannels that are manufactured on microchips. Separation is performed in the open channels, and the chips are transferred to a matrix-assisted laser desorption/ionization (MALDI) source after the solvent is evaporated. The matrix (2,5-dihydroxybenzoic acid) is placed in the solution with the run buffer before the separation of the analyte components. After separation, the solvent is evaporated and the microchip is ready for MALDI-MS analysis. The microchip is placed directly into a specially designed ion source of an external source Fourier transform mass spectrometry instrument. Separation of simple mixtures containing oligosaccharides and peptides is shown.
Liu
J
J
Department of Chemistry, School of Engineering, University of California, Davis 95616, USA.
Tseng
K
K
Garcia
B
B
Lebrilla
C B
CB
Mukerjee
E
E
Collins
S
S
Smith
R
R
D000818:Animals; D003505:Cyclodextrins / Q000032:analysis / Q000302:isolation & purification; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D005583:Fourier Analysis; D005841:Gentisates; D062385:Hydroxybenzoates / Q000737:chemistry; D007609:Kallidin / Q000032:analysis / Q000302:isolation & purification; D008297:Male; D009844:Oligosaccharides / Q000032:analysis* / Q000302:isolation & purification; D051381:Rats; D017207:Rats, Sprague-Dawley; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation / Q000379:methods*; D047392:beta-Cyclodextrins
D003505:Cyclodextrins; D005841:Gentisates; D062385:Hydroxybenzoates; D009844:Oligosaccharides; D047392:beta-Cyclodextrins; D007609:Kallidin; C016549:maltohexaose; C031215:betadex; C010925:2,5-dihydroxybenzoic acid