title: Protein overexport in a Saccharomyces cerevisiae mutant depends on mitochondrial genome integrity and function.
abstract: The wild-type yeast Saccharomyces cerevisiae (S. cerevisiae) is able to export less than 1 percent of the protein to be secreted. The reasons for retention of most of the secretory proteins on the cell surface of S. cerevisiae are unknown. Recently, temperature-sensitive (ts) mutants of S. cerevisiae showing an oversecretion phenotype were isolated. In order to study the influence of the mitochondrial genome status on protein export in yeast cells, we have isolated several types of respiratory impaired mitochondrial mutants of either the parental S. cerevisiae strain or their derivative ts protein-overexporting mutants. In this paper we demonstrate by quantitative analyses of exported proteins and by SDS-PAGE analysis that protein overexport in ts mutants requires mitochondrial genome integrity and function.
authors.affiliation: Institute of Molecular Biology, Bulgarian Academy of Science, Sofia.
authors.forename: I
authors.initials: I
authors.lastname: Alexandar
authors.forename: P
authors.initials: P
authors.lastname: Venkov
authors.forename: A
authors.initials: A
authors.lastname: Del Giudice
authors.forename: K
authors.initials: K
authors.lastname: Wolf
authors.forename: D R
authors.initials: DR
authors.lastname: Massardo
authors.forename: L
authors.initials: L
authors.lastname: Del Giudice
mesh_terms: D015139:Blotting, Southern; D004272:DNA, Mitochondrial / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000378:metabolism*; D008928:Mitochondria / Q000235:genetics / Q000502:physiology*; D016296:Mutagenesis; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism / Q000502:physiology*
chemical_list: D004272:DNA, Mitochondrial; D005656:Fungal Proteins