A standard of AmpliType PM typing from aged evidentiary samples.
In analyzing aged samples by the AmpliType PM PCR amplification and Typing kit, it was occasionally observed that color developed typing strips had dark allele dots on PM loci but no visible S dot. Since the S dot acts as a minimum dot intensity control to determine positive alleles on the PM loci, it is necessary to apply another control system. To achieve positive PM typing from a degraded DNA sample that is inferred to be derived from a single donor, a standard has been adopted wherein loci from which sufficient PCR products are observed on agarose gel can be typed. The objective determination of sufficient PCR was done by comparison between band peak height of each locus generated from a sample and that of the corresponding locus generated from two nanograms (recommended minimum quantity as template DNA) of the control DNA provided in the kit.
Mizuno
N
N
Division First Forensic Science, National Research Institute of Police Science, Kashiwa, Chiba, Japan. mizuno@nrips.go.jp
Senju
H
H
Sekiguchi
K
K
Yoshida
K
K
Kasai
K
K
Sato
H
H
D016477:Artifacts; D016172:DNA Fingerprinting / Q000379:methods*; D005554:Forensic Medicine / Q000379:methods; D006801:Humans; D016133:Polymerase Chain Reaction; D015203:Reproducibility of Results; D013048:Specimen Handling; D020080:Tandem Repeat Sequences / Q000235:genetics*; D013997:Time Factors