Characterization of three members of murine alpha1,2-fucosyltransferases: change in the expression of the Se gene in the intestine of mice after administration of microbes.
We cloned three members of a GDP-fucose:beta-galactoside alpha1,2-fucosyltransferase (alpha1,2-fucosyltransferase) family, MFUT-I, -II, and -III, from a cDNA of murine small intestine, and determined their enzymatic properties after transfection of the genes into COS-7 cells, and their expression in murine tissues by Northern blotting. MFUT-I, -II, and -III exhibited sequence homology with the human H (78.4%), Se (79.0%), and Sec1 (74.9%) gene products, respectively. COS-7 cells transfected with MFUT-I and -II exhibited alpha1,2-fucosyltransferase activity and the best acceptor substrate for both gene products was GA1 to yield a fucosyl GA1 structure, but no activity was detected in COS-7 cells with MFUT-III. MFUT-II yielded a 3.5-kb mRNA transcript in several tissues, whereas MFUT-I and -III were predominantly expressed in epididymis and testis, respectively. The administration of microbes into germ-free mice resulted in a rapid increase of the MFUT-II gene (Se gene) for the synthesis of fucosyl GA1 in the intestine.
Lin
B
B
Department of Biochemistry and Molecular Biology, Graduate School of Medicine, University of Tokyo, Japan.
Saito
M
M
Sakakibara
Y
Y
Hayashi
Y
Y
Yanagisawa
M
M
Iwamori
M
M
D000595:Amino Acid Sequence; D000818:Animals; D000942:Antigens, Bacterial / Q000737:chemistry; D000947:Antigens, Helminth; D001483:Base Sequence; D019556:COS Cells; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis; D005647:Fucosyltransferases / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D015801:Helminth Proteins / Q000737:chemistry; D006801:Humans; D007422:Intestines / Q000201:enzymology* / Q000382:microbiology; D051379:Mice; D008969:Molecular Sequence Data; D051938:Munc18 Proteins; D009419:Nerve Tissue Proteins / Q000737:chemistry; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014018:Tissue Distribution; D014162:Transfection; D033921:Vesicular Transport Proteins; D000097631:Galactoside 2-alpha-L-fucosyltransferase
D000942:Antigens, Bacterial; D000947:Antigens, Helminth; D004247:DNA; D005647:Fucosyltransferases; D015801:Helminth Proteins; D051938:Munc18 Proteins; D009419:Nerve Tissue Proteins; D033921:Vesicular Transport Proteins; D000097631:Galactoside 2-alpha-L-fucosyltransferase; C022863:H antigen; C079442:gp50 protein, Schistosoma