Identification of a novel T-cell epitope in soluble egg antigen of Schistosoma japonicum.
Identification of T-cell epitopes harbored in soluble egg antigen (SEA) of Schistosoma japonicum and study of the immunological properties are essential for understanding the immunopathology and the control of schistosomiasis. As a follow-up to our previous work, the 66- to 80-kDa fragment from SEA was partially digested with protease, fractionated by reverse-phase high-pressure liquid chromatography, and found to be carrying a peptide which stimulated proliferation and gamma interferon (IFN-gamma) production of Th1 clones specific to SEA. Sequence analysis showed that the peptide was composed of 12 amino acids lined up as DLAVELAYLGNL. A synthetic homologue induced proliferation and IFN-gamma and interleukin-2 (IL-2) production, but not IL-4 or IL-6 production, by the Th1 clones as well as by the spleen cells from SEA-immunized mice, thus indicating that the peptide carries a Th1 epitope of SEA.
Liu
J
J
Department of Parasitology and Immunology, Yamanashi Medical University, Yamanashi 409-3898, Japan.
Tasaka
K
K
Yang
J
J
Itoh
T
T
Yamada
M
M
Yoshikawa
H
H
Nakajima
Y
Y
D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology*; D002999:Clone Cells; D016207:Cytokines / Q000378:metabolism; D018984:Epitopes, T-Lymphocyte / Q000276:immunology*; D007114:Immunization; D051379:Mice; D008809:Mice, Inbred C3H; D010063:Ovum / Q000276:immunology*; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology; D012549:Schistosoma japonicum / Q000276:immunology*; D013154:Spleen / Q000166:cytology / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology
D000947:Antigens, Helminth; D016207:Cytokines; D018984:Epitopes, T-Lymphocyte; D010455:Peptides