Antibody responses of Wuchereria bancrofti patients to recombinant Brugia pahangi superoxide dismutase.
Lymphatic filarial parasite Brugia malayi contains significant amount of Cu/Zn superoxide dismutase (SOD) activity in the extract of different life stages and in the excretory-secretory product of adults. In the present study recombinant SOD from B. pahangi has been used to see the antibody response in Wuchereria bancrofti infected patients. The recombinant SOD from B. pahangi reacted specifically with W. bancrofti infected sera in ELISA and immunoblotting. The reactivity of IgM subclass was more as compared to IgG subclass both in the asymptomatic microfilaraemic and symptomatic amicrofilaraemic when tested by ELISA. Serum from other helminthic infection was very low and found to be insignificant. The antibody response to rec SOD was directly proportional to the number of microfilariae in infected patients. The circulating filarial SOD was detected in filarial patients using polyclonal antibodies raised against recombinant Cu/Zn SOD in rabbits. The apparent molecular masses as determined by immunoblotting were 29 and 22 kDa. The specificity of recombinant SOD could be explored for its use in immunodiagnosis of lymphatic filariasis.
Rathaur
S
S
Department of Biochemistry, Faculty of Science, Banaras Hindu University, Varanasi 221 005, India. sushma@banaras.ernet.in
Sharma
S
S
Singh
R N
RN
Henkle
K
K
Selkirk
M E
ME
D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis*; D015153:Blotting, Western; D017179:Brugia pahangi / Q000201:enzymology*; D004797:Enzyme-Linked Immunosorbent Assay; D005368:Filariasis / Q000276:immunology*; D006801:Humans; D011994:Recombinant Proteins / Q000494:pharmacology; D013482:Superoxide Dismutase / Q000494:pharmacology*; D014958:Wuchereria bancrofti / Q000302:isolation & purification*
D000909:Antibodies, Helminth; D011994:Recombinant Proteins; D013482:Superoxide Dismutase