[Study of mechanism of transformation of ATP-G actin to F actin in Mg(2+)-containing solutions by UV absorption spectra].
The mechanism of transformation of ATP-G actin to F actin by the action of Mg2+ ions was studied by measuring UV absorption spectra of solutions of monomeric Ca(2+)-ATP-G actin in the presence of MgCl2 and of Mg(2+)-ATP-G actin. The protein solutions contained no excessive free ATP. The changes in the UV absorption spectra of monomeric ATP-G actin that result from the interaction with Mg2+ are explained by the formation of ADP-G actin from monomeric ATP-G actin due to hydrolysis of ATP which is considered as the initial step of the transformation to F actin.
Umetskaia
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VN
Institute of Cytology, Russian Academy of Sciences, Tikhoretskii 4, St. Petersburg, 194064 Russia.
Pinaev
G P
GP
D000199:Actins / Q000737:chemistry*; D000255:Adenosine Triphosphate / Q000031:analogs & derivatives / Q000737:chemistry*; D002413:Cations, Divalent; D008274:Magnesium; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet
C022920:ATP-G-actin; D000199:Actins; D002413:Cations, Divalent; D012996:Solutions; D000255:Adenosine Triphosphate; D008274:Magnesium