[Neurotoxicity of antibody against motoneuron on cultured rat cortical neurons].
OBJECTIVE
In order to determine the role of antibody against motoneuron in the process of neuronal death.


METHODS
Cultured rat cortical neurons growing in serum-free medium, were employed to observe the effects of antibody against motoneuron (anti-SMN) on the survival of these cultured neurons.


RESULTS
(1) Exposure of the cultures in serum-free medium to anti-SMN with dilution of 1:50 results in death of 31%-41% of neurons for 24 hours incubation, 50%-67% for 48 hours and above 90% after 72 hours. The neurotoxicity induced by anti-SMN was accompanied by concentration-dependent release of lactate dehydrogenase into the culture medium. Cultures exposed to normal rat IgG as controls exhibited no such sign. (2) The cultured neurons exposed to anti-SMN (1:200) expressed markedly higher levels of immunoreactive Calbindin-D28k especially after 48 hours incubation with it. There was a reduction in the number of acetylcholinesterase-positive neurons compared to the normal control.


CONCLUSIONS
The anti-SMN could directly initiate the process of cortical neuronal death and this effect was independent of complement. The alteration of Calbindin-D28k immunoreactivity implied that calcium may be involved in the neurotoxicity induced by anti-SMN.
Gao
Y
Y
Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005.
Li
Y
Y
Lin
J
J
Li
L
L
Wan
X
X
D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D001323:Autoantibodies / Q000276:immunology*; D064092:Calbindin 1; D064026:Calbindins; D002470:Cell Survival; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology*; D009046:Motor Neurons / Q000276:immunology*; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D064030:S100 Calcium Binding Protein G / Q000378:metabolism
D001323:Autoantibodies; C576500:Calb1 protein, rat; D064092:Calbindin 1; D064026:Calbindins; D009419:Nerve Tissue Proteins; D064030:S100 Calcium Binding Protein G; D000110:Acetylcholinesterase