Class II-targeted antigen is superior to CD40-targeted antigen at stimulating humoral responses in vivo.
We examined the efficacy of using monoclonal antibodies to target antigen (avidin) to different surface molecules expressed on antigen presenting cells (APC). In particular, we targeted CD40 to test whether the "adjuvant" properties of CD40 signaling combined with targeted antigen would result in enhanced serologic responses. We targeted avidin to class II as a positive control and to CD11c as a negative control. These surface proteins represent an ensemble of surface molecules that signal upon ligation and that are expressed on professional APC, in particular dendritic cells (DC). We observed that targeting class II molecules on APC was superior to targeting CD40, or CD11c. However, CD40 and CD11c could function as targets for antigen bound monoclonal antibodies under certain conditions. Interestingly, inclusion of anti-CD40 mAb with the targeting anti-class II-targeted antigens negatively affects humoral response, suggesting that CD40 signaling under certain conditions may suppress processing and/or presentation of targeted antigen.
Frleta
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D
Department of Microbiology, Dartmouth Medical School, Lebanon, NH 03756, USA.
Demian
D
D
Wade
W F
WF
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000917:Antibody Formation; D000938:Antigen-Presenting Cells / Q000502:physiology; D001360:Avidin / Q000276:immunology*; D019013:CD40 Antigens / Q000502:physiology*; D005260:Female; D000949:Histocompatibility Antigens Class II / Q000502:physiology*; D007114:Immunization; D016167:Integrin alphaXbeta2 / Q000502:physiology; D051379:Mice; D008808:Mice, Inbred CBA
D000911:Antibodies, Monoclonal; D019013:CD40 Antigens; D000949:Histocompatibility Antigens Class II; D016167:Integrin alphaXbeta2; D001360:Avidin