A Hodgkin's disease cell line, KM-H2, shows biphenotypic features of dendritic cells and B cells.
The origin of Reed-Sternberg (RS) cells, the neoplastic cells of Hodgkin's disease, has long remained controversial. Dendritic cells (DCs) are highly specialized antigen-presenting cells that have the unique capacity to prime naive T cells, and they may be progenitors of RS cells in a population of Hodgkin's disease cells. In this study, the KM-H2 cell line, previously established from a patient with Hodgkin's disease of mixed cellularity, was reevaluated for its cellular derivation, particularly in terms of DCs. KM-H2 cells were demonstrated to carry the newly proposed DC-associated molecules fascin, CD83, and DEC-205, as well as costimulatory molecules such as CD40, CD80, and CD86. In addition, KM-H2 cells were shown to be able to potently stimulate peripheral blood T cells and to have the strong endocytotic activity of fluorescein isothiocyanate-dextran. On the other hand, KM-H2 cells were shown to have variable-diversity-joining recombination of the immunoglobulin H gene, although they did not express any subclasses of immunoglobulin and they were negative for CD79a and CD79b. In addition, KM-H2 cells produced the messenger RNA of the Pax-5 gene. These findings lead to a hypothesis that KM-H2 cells originated from the cells that had differentiated through the possible common DC-B-cell progenitors along the newly proposed pathway.
Uehira
K
K
First Department of Internal Medicine, Kansai Medical University, Osaka, Japan.
Amakawa
R
R
Ito
T
T
Uehira
T
T
Ozaki
Y
Y
Shimizu
T
T
Fujimoto
M
M
Inaba
M
M
Fukuhara
S
S
D000328:Adult; D015703:Antigens, CD; D001402:B-Lymphocytes / Q000473:pathology*; D002352:Carrier Proteins / Q000378:metabolism; D019070:Cell Lineage; D004268:DNA-Binding Proteins / Q000378:metabolism; D003713:Dendritic Cells / Q000473:pathology*; D005434:Flow Cytometry; D006689:Hodgkin Disease / Q000473:pathology*; D006801:Humans; D016130:Immunophenotyping; D037181:Lectins, C-Type; D007959:Lymphocyte Culture Test, Mixed; D008297:Male; D008562:Membrane Glycoproteins / Q000378:metabolism; D008840:Microfilament Proteins / Q000378:metabolism; D015778:Minor Histocompatibility Antigens; D051757:PAX5 Transcription Factor; D011956:Receptors, Cell Surface / Q000378:metabolism; D016539:Reed-Sternberg Cells / Q000473:pathology; D014157:Transcription Factors / Q000378:metabolism; D014407:Tumor Cells, Cultured / Q000737:chemistry / Q000473:pathology*
D015703:Antigens, CD; D002352:Carrier Proteins; C093569:DEC-205 receptor; D004268:DNA-Binding Proteins; D037181:Lectins, C-Type; D008562:Membrane Glycoproteins; D008840:Microfilament Proteins; D015778:Minor Histocompatibility Antigens; D051757:PAX5 Transcription Factor; C497281:PAX5 protein, human; D011956:Receptors, Cell Surface; D014157:Transcription Factors; C020324:fascin