title: Separation of apple procyanidins into different degrees of polymerization by high-speed counter-current chromatography.
abstract: Apple procyanidins were fractionated by high-speed counter-current chromatography in a one-step operation from apple condensed tannins using a type-J multilayer coil planet centrifuge. The separation of procyanidins was performed with a two-phase solvent system composed of methyl acetate-water (1:1) by eluting the upper phase at a flow-rate of 1.0 ml/min. Each fraction was examined by time-of-flight mass spectrometry. Procyanidins were separated according to their degrees of polymerization.
authors.affiliation: Department of Analytical Chemistry, School of Pharmacy, Tokyo University of Pharmacy and Life Science, Hachioji, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Shibusaw
authors.forename: A
authors.initials: A
authors.lastname: Yanagida
authors.forename: M
authors.initials: M
authors.lastname: Isozaki
authors.forename: H
authors.initials: H
authors.lastname: Shindo
authors.forename: Y
authors.initials: Y
authors.lastname: Ito
mesh_terms: D044946:Biflavonoids; D002392:Catechin / Q000737:chemistry / Q000302:isolation & purification*; D003377:Countercurrent Distribution / Q000379:methods*; D011108:Polymers / Q000737:chemistry; D044945:Proanthocyanidins; D019685:Rosales / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D044946:Biflavonoids; D011108:Polymers; D044945:Proanthocyanidins; C017674:procyanidin; D002392:Catechin