title: Histomorphometric description of allograft bone remodeling and union in a canine segmental femoral defect model: a comparison of rhBMP-2, cancellous bone graft, and absorbable collagen sponge.
abstract: The purpose of this study was to determine the effect of recombinant human bone morphogenetic protein type 2 (rhBMP-2) on the histomorphometry of femoral allograft-host bone union and allograft remodeling. A 6 cm mid-diaphyseal femoral defect was created and filled with an allograft stabilized with an interlocking nail in 21 dogs. Dogs were randomly divided into three equal groups and the allograft-host bone junctions and the mid-diaphyses of the allografts were treated with either an absorbable collagen sponge (ACS) loaded with rhBMP-2 (BMP group), an autogenous cancellous bone graft (CBG group), or ACS loaded with buffer solution (ACS group). All dogs received daily tetracycline until sacrifice at 24 weeks to label new bone formation. Histomorphometric analyses on sections of proximal and distal allograft-host bone junctions and the mid-diaphyseal portion of allografts were performed using fluorescent and regular light microscopy. Analyses of the host bone and junctions between allograft and host bone revealed significantly greater new bone formation and larger osteon radii in the BMP group compared to CBG and ACS groups and contralateral intact bone. Porosity in CBG and ACS groups was significantly higher than in the BMP group, which had similar values to intact bone. In transverse sections of allografts, the largest pore diameters were present in the CBG group. Based on all parameters measured, significantly higher bone turnover occurred in the outer cortical area of the allograft in all groups as compared to the inner cortical and mid-cortical areas. New bone formation and osteon radius/osteon width in allografts were similar for all three groups. Higher porosity and larger pore diameters in the CBG and ACS groups suggested higher bone resorption versus formation in these groups compared to the BMP group. The results of this study reveal more balanced allograft bone resorption and bone formation in the BMP group, with greater resorptive activity in the CBG and ACS groups. However, neither rhBMP-2 nor autogenous bone graft increased allograft incorporation when compared to the negative control (ACS group).
authors.affiliation: Department of Medical Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, 53706, USA.
authors.forename: A G
authors.initials: AG
authors.lastname: Zabka
authors.forename: G E
authors.initials: GE
authors.lastname: Pluhar
authors.forename: R B
authors.initials: RB
authors.lastname: Edwards
authors.forename: P A
authors.initials: PA
authors.lastname: Manley
authors.forename: K
authors.initials: K
authors.lastname: Hayashi
authors.forename: J P
authors.initials: JP
authors.lastname: Heiner
authors.forename: V L
authors.initials: VL
authors.lastname: Kalscheur
authors.forename: H J
authors.initials: HJ
authors.lastname: Seeherman
authors.lastname: Markel
mesh_terms: D020341:Absorbable Implants; D000818:Animals; D001847:Bone Diseases / Q000601:surgery*; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000627:therapeutic use; D016723:Bone Remodeling / Q000187:drug effects; D016025:Bone Transplantation; D003094:Collagen; D004285:Dogs; D005260:Female; D005269:Femur / Q000187:drug effects / Q000503:physiopathology* / Q000601:surgery*; D006801:Humans; D011161:Porifera; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; D014184:Transplantation, Homologous
chemical_list: C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; C499469:recombinant human bone morphogenetic protein-2; D003094:Collagen