New crystal forms of Trypanosoma cruzi dUTPase.
The dUTPase from Trypanosoma cruzi has been crystallized in two crystal forms, both belonging to space group P6(3)22, with unit-cell parameters a = b = 134.67, c = 148.66 A (form I, two molecules per asymmetric unit) and a = b = 136.43, c = 68.71 A (form II, one molecule per asymmetric unit). Single-wavelength data have been collected using synchrotron radiation to 3.0 A for crystal form I and to 2.4 A for crystal form II and structure solution is under way. T. cruzi dUTPase is a potential target for anti-protozoan drug design.
Harkiolaki
M
M
Structural Biology Laboratory, Department of Chemistry, University of York, Heslington, York YO10 5DD, England.
Brzozowski
A M
AM
Gonzalez-Pacanowska
D
D
Hidalgo-Zarco
F
F
Wilson
K S
KS
D000818:Animals; D003460:Crystallization; D018360:Crystallography, X-Ray; D011487:Protein Conformation; D011755:Pyrophosphatases / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry; D014349:Trypanosoma cruzi / Q000201:enzymology*
D011994:Recombinant Proteins; D011755:Pyrophosphatases; C020020:dUTP pyrophosphatase