title: On-line sample preparation for high throughput reversed-phase LC/MS analysis of combinatorial chemistry libraries.
abstract: An on-line sample preparation method utilizing a time-programmed autosampler is described for high throughput liquid chromatography/mass spectrometry (LC/MS). This approach is particularly helpful for the LC/MS analysis of samples which require solvents incompatible with HPLC in the sample preparation process. The on-line sample preparation approach minimizes a bottleneck in throughput and improves sample recovery under some circumstances.
authors.affiliation: Affymax Research Institute, 4001 Miranda Avenue, Palo Alto, CA 94304, USA. ltang@tularik.com
authors.forename: L
authors.initials: L
authors.lastname: Tang
authors.forename: W L
authors.initials: WL
authors.lastname: Fitch
authors.forename: P
authors.initials: P
authors.lastname: Smith
authors.forename: D
authors.initials: D
authors.lastname: Tumelty
authors.forename: K
authors.initials: K
authors.lastname: Cao
authors.forename: S W
authors.initials: SW
authors.lastname: Ferla
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D020650:Combinatorial Chemistry Techniques; D013058:Mass Spectrometry / Q000379:methods*; D009862:Online Systems