UVB irradiation suppresses cytokine production and innate cellular immune functions in mice.
We examined whether ultraviolet-B (UVB) irradiation (6 kJ/m2) alters cytokine production and other innate immune reactions by murine peritoneal macrophages and peripheral neutrophils. Along with these experiments, serum IgG levels were also assessed. In addition, using scanning electron microscopy (SEM) we observed macrophages that had been exposed to UVB in vitro. Results showed that UVB irradiation: (1) decreased IL-12 production while increasing IL-1alpha secretion from macrophages, but had no effect on IL-1alpha from neutrophils; (2) suppressed phagocytosis of macrophages but not of neutrophils; (3) diminished active oxygen production of macrophages but not of neutrophils; (4) had no effect on serum IgG levels; and (5) caused significant cell destruction of macrophages in vitro. These results suggested: (1) that UVB irradiation could induce characteristic suppression of innate immunity; (2) that innate cellular immunity was more susceptible to the effects of UVB irradiation than humoral immunity.
Kasahara
S
S
Laboratory of Comparative Immunology, UCLA Medical Center, Los Angeles, California 90095-1763, USA.
Aizawa
K
K
Okamiya
M
M
Kazuno
N
N
Mutoh
S
S
Fugo
H
H
Cooper
E L
EL
Wago
H
H
D000818:Animals; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D004912:Erythrocytes / Q000276:immunology / Q000528:radiation effects; D005260:Female; D007074:Immunoglobulin G / Q000097:blood; D007375:Interleukin-1 / Q000096:biosynthesis*; D018664:Interleukin-12 / Q000096:biosynthesis*; D008247:Lysosomes / Q000201:enzymology / Q000528:radiation effects; D017737:Macrophages, Peritoneal / Q000276:immunology* / Q000378:metabolism / Q000528:radiation effects* / Q000648:ultrastructure; D051379:Mice; D008807:Mice, Inbred BALB C; D008855:Microscopy, Electron, Scanning; D009504:Neutrophils / Q000166:cytology / Q000276:immunology* / Q000378:metabolism / Q000528:radiation effects*; D010100:Oxygen / Q000378:metabolism; D010587:Phagocytosis / Q000528:radiation effects; D012756:Sheep; D014466:Ultraviolet Rays; D015003:Yeasts / Q000276:immunology / Q000528:radiation effects
D007074:Immunoglobulin G; D007375:Interleukin-1; D018664:Interleukin-12; D010100:Oxygen