Enhanced systemic and mucosal antibody responses to a model protein antigen after intranasal and intratracheal immunisation using Bacillus firmus as an adjuvant.
Bacillus firmus, a non-pathogenic Gram positive (G+) bacterium of the external environment was investigated for immunomodulatory properties. It stimulated an increase in anti-ovalbumin IgG in sera, bronchoalveolar lavages and intestinal washings after both intranasal (i.n.) and intratracheal (i.t.) immunisation, and enhanced anti-ovalbumin IgA in intestinal secretions and in bronchoalveolar lavage fluid after i.n. or i.t. immunisation, respectively. The immunomodulatory effect of B. firmus on antibody formation was antigen specific.
Mlcková
P
P
Charles University, First Faculty of Medicine, Institute of Clinical Immunology, Studnickova7, Prague 2 12800, Czech Republic. ppisk@lfl.cuni.cz
Cechová
D
D
Chalupná
P
P
Novotná
O
O
Prokesová
L
L
D000276:Adjuvants, Immunologic; D000281:Administration, Intranasal; D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000907:Antibodies, Bacterial / Q000097:blood; D000917:Antibody Formation; D000941:Antigens / Q000276:immunology; D001407:Bacillus / Q000276:immunology*; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology; D005260:Female; D018928:Immunity, Mucosal / Q000276:immunology; D007267:Injections; D007413:Intestinal Mucosa / Q000276:immunology*; D007422:Intestines / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000276:immunology; D020545:Respiratory Mucosa / Q000276:immunology*; D014132:Trachea
D000276:Adjuvants, Immunologic; D000906:Antibodies; D000907:Antibodies, Bacterial; D000941:Antigens; D010047:Ovalbumin