Induction of unspliced c-fos messenger RNA in rodent brain by kainic acid and lipopolysaccharide.
The c-fos transcriptional factor forms an activator protein-1 (AP-1) complex with proteins from the Jun family, which plays an important role in the central nervous system. The responses of AP-1 transcriptional factors induced by kainic acid (KA) treatment have been well studied, although the transcriptional regulation of these KA-induced factors has not been clearly characterized. To investigate the role of different stimuli in controlling of the splicing of c-fos mRNA, we performed reverse transcriptional polymerase chain reaction. The results showed that spliced and unspliced c-fos is present in rat brain following KA treatment and in lipopolysaccharide (LPS)-treated primary mouse cortical brain cell cultures. Furthermore, tyrosine kinase and protein phosphatase inhibitors alter the preponderance of c-fos transcripts following LPS treatment.
Feng
Z
Z
Division of Neurology, University Department of Medicine, University of Hong Kong, Queen Mary Hospital, Hong Kong
Kong
L Y
LY
Qi
Q
Q
Ho
S L
SL
Tiao
N
N
Bing
G
G
Han
Y F
YF
D000818:Animals; D001483:Base Sequence / Q000235:genetics; D001921:Brain / Q000187:drug effects* / Q000378:metabolism*; D004274:DNA, Recombinant; D018690:Excitatory Amino Acid Agonists / Q000494:pharmacology*; D007608:Kainic Acid / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008297:Male; D008969:Molecular Sequence Data; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D011916:Rats, Inbred F344; D020133:Reverse Transcriptase Polymerase Chain Reaction
D004274:DNA, Recombinant; D018690:Excitatory Amino Acid Agonists; D008070:Lipopolysaccharides; D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger; D007608:Kainic Acid