title: Long-term persistence and recall of immune responses in aged mice after mucosal immunization.
abstract: To evaluate the retention of memory in the mucosal immune system of aged animals, 2-year-old mice that had been immunized intragastrically at 3 months of age with Streptococcus mutans protein AgI/II coupled to the B subunit of cholera toxin (CTB) were evaluated by ELISA for antibodies to AgI/II and CT in serum, saliva, and vaginal wash. To evaluate recall responses, mice were then immunized intragastrically with AgI/II-CTB, in comparison with previously unimmunized controls. Those that had been primed in their youth showed a more rapid antibody response in serum (immunoglobulin G (IgG)) and secretions (IgA), but all animals eventually responded to a similar degree after the third dose. Mice immunized at 3 months also retained for 2 years spleen cells capable of proliferating in vitro in response to AgI/II. These data show that aged mice retain the ability to mount immune responses to mucosally presented immunogens and that memory to mucosally presented immunogens can persist for almost the whole lifetime of a mouse.
authors.affiliation: Department of Microbiology, University of Alabama at Birmingham, USA.
authors.forename: T
authors.initials: T
authors.lastname: Harrod
authors.forename: M
authors.initials: M
authors.lastname: Martin
authors.forename: M W
authors.initials: MW
authors.lastname: Russell
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000282:Administration, Intravaginal; D000375:Aging / Q000276:immunology*; D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis / Q000097:blood; D000942:Antigens, Bacterial / Q000008:administration & dosage / Q000276:immunology; D001426:Bacterial Proteins / Q000008:administration & dosage / Q000276:immunology; D002772:Cholera Toxin / Q000008:administration & dosage / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D018928:Immunity, Mucosal / Q000276:immunology*; D007114:Immunization; D007117:Immunization, Secondary; D007071:Immunoglobulin A, Secretory / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis / Q000097:blood; D007156:Immunologic Memory / Q000276:immunology*; D016014:Linear Models; D051379:Mice; D008807:Mice, Inbred BALB C; D012463:Saliva / Q000276:immunology; D013154:Spleen / Q000276:immunology; D013223:Statistics as Topic; D013270:Stomach; D013295:Streptococcus mutans / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D014621:Vagina / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D007071:Immunoglobulin A, Secretory; D007074:Immunoglobulin G; C081813:wapA protein, Bacillus subtilis; D002772:Cholera Toxin