title: CD147 facilitates HIV-1 infection by interacting with virus-associated cyclophilin A.
abstract: Cyclophilin A (CyPA) is specifically incorporated into the virions of HIV-1 and has been shown to enhance significantly an early step of cellular HIV-1 infection. Our preliminary studies implicated CD147 as a receptor for extracellular CyPA. Here, we demonstrate a role for CyPA-CD147 interaction during the early steps of HIV-1 infection. Expression of human CD147 increased infection by HIV-1 under one-cycle conditions. However, susceptibility to infection by viruses lacking CyPA (simian immunodeficiency virus or HIV-1 produced in the presence of cyclosporin A) was unaffected by CD147. Virus-associated CyPA coimmunoprecipitated with CD147 from infected cells. Antibody to CD147 inhibited HIV-1 entry as evidenced by the delay in translocation of the HIV-1 core proteins from the membrane and inhibition of viral reverse transcription. Viruses whose replication did not require CyPA (SIV or mutant HIV-1) were resistant to the inhibitory effect of anti-CD147 antibody. These results suggest that HIV-1 entry depends on an interaction between virus-associated CyPA and CD147 on a target cell.
authors.affiliation: The Picower Institute for Medical Research, 350 Community Drive, Manhasset, NY 11030, USA.
authors.forename: T
authors.initials: T
authors.lastname: Pushkarsky
authors.forename: G
authors.initials: G
authors.lastname: Zybarth
authors.forename: L
authors.initials: L
authors.lastname: Dubrovsky
authors.forename: V
authors.initials: V
authors.lastname: Yurchenko
authors.forename: H
authors.initials: H
authors.lastname: Tang
authors.forename: H
authors.initials: H
authors.lastname: Guo
authors.forename: B
authors.initials: B
authors.lastname: Toole
authors.forename: B
authors.initials: B
authors.lastname: Sherry
authors.forename: M
authors.initials: M
authors.lastname: Bukrinsky
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism; D015703:Antigens, CD; D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D030161:Avian Proteins; D051926:Basigin; D001798:Blood Proteins; D016466:CHO Cells; D006224:Cricetinae; D021984:Cyclophilin A / Q000378:metabolism*; D015497:HIV-1 / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D030161:Avian Proteins; C497886:BSG protein, human; D001798:Blood Proteins; C102624:Bsg protein, Gallus gallus; C079432:Bsg protein, rat; D008562:Membrane Glycoproteins; D051926:Basigin; D021984:Cyclophilin A