title: CENTA as a chromogenic substrate for studying beta-lactamases.
abstract: CENTA, a chromogenic cephalosporin, is readily hydrolyzed by beta-lactamases of all classes except for the Aeromonas hydrophila metalloenzyme. Although it cannot practically be used for the detection of beta-lactamase-producing strains on agar plates, it should be quite useful for kinetic studies and the detection of the enzymes in crude extracts and chromatographic fractions.
authors.affiliation: Centre for Protein Engineering, University of Liège, Institut de Chimie B6, Sart Tilman, 4000 Liège, Belgium.
authors.forename: C
authors.initials: C
authors.lastname: Bebrone
authors.forename: C
authors.initials: C
authors.lastname: Moali
authors.forename: F
authors.initials: F
authors.lastname: Mahy
authors.forename: S
authors.initials: S
authors.lastname: Rival
authors.forename: J D
authors.initials: JD
authors.lastname: Docquier
authors.forename: G M
authors.initials: GM
authors.lastname: Rossolini
authors.forename: J
authors.initials: J
authors.lastname: Fastrez
authors.forename: R F
authors.initials: RF
authors.lastname: Pratt
authors.forename: J M
authors.initials: JM
authors.lastname: Frère
authors.forename: M
authors.initials: M
authors.lastname: Galleni
mesh_terms: D002511:Cephalosporins; D002863:Chromogenic Compounds; D007202:Indicators and Reagents; D007700:Kinetics; D009169:Mycobacterium tuberculosis / Q000201:enzymology; D011550:Pseudomonas aeruginosa / Q000201:enzymology; D013211:Staphylococcus aureus / Q000201:enzymology; D001618:beta-Lactamases / Q000378:metabolism*
chemical_list: D002511:Cephalosporins; D002863:Chromogenic Compounds; D007202:Indicators and Reagents; C034025:CENTA; D001618:beta-Lactamases; C021720:nitrocefin