title: Chemical characteristics and relative bioavailability of supplemental organic copper sources for poultry.
abstract: Five commercially available organic Cu products and reagent-grade CuSO4 x 5H2O (Cu Sulf) were evaluated by polarographic analysis and solubility in 0.1 M K2HPO4-KH2PO4 buffer (pH 5), 0.2 M HCl-KCl buffer (pH 2), or deionized water. Fractions from these solubility tests were evaluated by gel filtration chromatography for structural integrity. The organic sources were Cu lysine complex (Cu Lys), Cu amino acid chelate (Cu AA), Cu proteinate A (Cu ProA), Cu proteinate B (Cu ProB), and Cu proteinate C (Cu ProC). Separation of peaks in the chromatograms for the soluble Cu fraction from deionized water indicated that 77, 31, 69, 94, and 16% of the Cu remained chelated for the above sources, respectively. Two experiments were conducted to estimate the relative bioavailability of Cu from the organic Cu supplements for chicks when added at high dietary concentrations to practical corn-soybean meal diets. Liver Cu concentration increased (P < 0.0001) as dietary Cu increased in both experiments. When Cu Sulf was assigned a value of 100% as the standard, linear regression slope ratios of log10 liver Cu concentration regressed on added dietary Cu concentration gave estimated relative bioavailability values of 124 +/- 5.1, 122 +/- 5.3, and 111 +/- 6.0 for Cu Lys, Cu AA, and Cu ProC, respectively, in Exp. 1. The bioavailability estimates for Cu Lys and Cu AA were greater (P < 0.05) than that for Cu Sulf. Values in Exp. 2 were 111 +/- 7.6, 109 +/- 8.4, and 105 +/- 7.5 for Cu Lys, Cu ProA, and Cu ProB, respectively, and all sources were similar in value for chicks. Solubility of Cu in pH 2 buffer provided the best prediction of bioavailability (r2 = 0.924). Other indicators of chelation integrity and solubility had little value as predictors of bioavailability (r2 < or = 0.445).
authors.affiliation: Department of Animal Sciences, University of Florida, Gainesville 32611-0900, USA.
authors.forename: R
authors.initials: R
authors.lastname: Guo
authors.forename: P R
authors.initials: PR
authors.lastname: Henry
authors.forename: R A
authors.initials: RA
authors.lastname: Holwerda
authors.forename: J
authors.initials: J
authors.lastname: Cao
authors.forename: R C
authors.initials: RC
authors.lastname: Littell
authors.forename: R D
authors.initials: RD
authors.lastname: Miles
authors.forename: C B
authors.initials: CB
authors.lastname: Ammerman
mesh_terms: D000818:Animals; D001682:Biological Availability; D002645:Chickens / Q000378:metabolism*; D003300:Copper / Q000493:pharmacokinetics*; D019327:Copper Sulfate / Q000493:pharmacokinetics; D004032:Diet / Q000662:veterinary*; D019587:Dietary Supplements; D004492:Edetic Acid / Q000493:pharmacokinetics; D008099:Liver / Q000378:metabolism; D008239:Lysine / Q000493:pharmacokinetics; D008297:Male; D009584:Nitrogen / Q000378:metabolism; D012995:Solubility
chemical_list: C053976:copper-lysine complex; D003300:Copper; D004492:Edetic Acid; D008239:Lysine; D019327:Copper Sulfate; D009584:Nitrogen