title: Design and synthesis of 3alpha-helix peptides forming a cavity for a fluorescent ligand.
abstract: As a model of receptor protein, a series of 3alpha-helix bundle peptides constructed on a template peptide were designed so as to possess a hydrophobic cavity. The size of cavity was modulated by simple replacements of Leu residues to Ala residues in the hydrophobic core. Binding abilities to 8-anilino-1-naphthalenesulfonic acid (ANS) were estimated by the increase of fluorescence intensity. The peptide having three or four Ala residues in the hydrophobic core remarkably increased the binding ability for ANS, though the peptide having two Ala residues gave an inefficient cavity for ANS. The peptide having six Ala residues decreased the binding ability due to crucial destabilization of the helix bundle structure. This scaffold can be utilized to a receptor model, while further tuning of the sequence is necessary.
authors.affiliation: Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta, Yokohama 226-8501, Japan.
authors.forename: I
authors.initials: I
authors.lastname: Obataya
authors.forename: S
authors.initials: S
authors.lastname: Sakamoto
authors.forename: A
authors.initials: A
authors.lastname: Ueno
authors.forename: H
authors.initials: H
authors.lastname: Mihara
mesh_terms: D000595:Amino Acid Sequence; D000817:Anilino Naphthalenesulfonates; D002942:Circular Dichroism; D015195:Drug Design; D005456:Fluorescent Dyes; D007930:Leucine; D008024:Ligands; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry*; D017433:Protein Structure, Secondary; D013050:Spectrometry, Fluorescence
chemical_list: D000817:Anilino Naphthalenesulfonates; D005456:Fluorescent Dyes; D008024:Ligands; D010455:Peptides; C027132:1-anilino-8-naphthalenesulfonate; D007930:Leucine