Molecular cloning and mapping of the human nicotinic acetylcholine receptor alpha10 (CHRNA10).
We report the isolation and initial characterization of a new member of the human nicotinic acetylcholine receptor (nAChR) subunit family, alpha10 (CHRNA10), from both inner-ear neuroepithelium and lymphoid tissue. The cDNA is 1959 nucleotides in length, with a coding region predicting a protein of 451 amino acids that is 90% identical to rat alpha10. The alpha10 gene was localized to chromosome 11p15.5. Human alpha10 was detected in human inner-ear tissue, tonsil, immortalized B-cells, cultured T-cells and peripheral blood lymphocytes using reverse transcriptase-polymerase chain reaction, Northern blot hybridization, and immunohistochemistry. We also detected the expression of the human nAChR alpha9 (CHRNA9) mRNA in these same tissues using RT-PCR and Northern blot hybridization.
Lustig
L R
LR
The Center for Hearing Sciences, Johns Hopkins University, Baltimore, Maryland 21205, USA. llustig@bme.jhu.edu
Peng
H
H
Hiel
H
H
Yamamoto
T
T
Fuchs
P A
PA
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D002460:Cell Line; D002880:Chromosomes, Human, Pair 11 / Q000235:genetics*; D003001:Cloning, Molecular; D005091:Exons / Q000235:genetics; D006801:Humans; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D007438:Introns / Q000235:genetics; D008969:Molecular Sequence Data; D021122:Protein Subunits; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D021101:Radiation Hybrid Mapping; D051381:Rats; D011978:Receptors, Nicotinic / Q000032:analysis / Q000737:chemistry / Q000235:genetics*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014162:Transfection
D021122:Protein Subunits; D012333:RNA, Messenger; D011978:Receptors, Nicotinic