Application of colloidal gold to ultrastructural studies.
Progress in recognition of structure and function in a variety of cells has always been linked to development of new research techniques. Beyond doubt, immunocytochemical techniques belong to such new methods. However, the techniques had not been widely applied to electron microscopy until colloidal gold was introduced as a label. This paper presents the pre-embedding and the post-embedding techniques and the applied markers, which are used in immunocytochemical techniques at the ultrastructural level. The potential of colloidal gold techniques is discussed. Particular attention is paid to methods of reaction amplification. Application of gold is illustrated with our own results.
Seidel
J
J
Department of Histology and Embryology, University of Medical Sciences, Poznań, Poland. josei@usoms.poznan.pl
Zabel
M
M
D000818:Animals; D002477:Cells / Q000648:ultrastructure*; D017979:Gold Colloid; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D016610:Tissue Embedding
D017979:Gold Colloid