title: [Role of Herpes simplex virus in the immune stromal keratitis].
abstract: BACKGROUND
  Herpes simplex virus (HSV) infection of the cornea is a leading cause of blindness in occidental countries and a common recurrent manifestation of it is the immune stromal keratitis (ISK). However, it is not known whether active viral replication occurs during the acute phase of the disease, because isolation of the virus by conventional culture techniques has not been accomplished.
  
  
  OBJECTIVE
  To establish the presence of HSV in patients with ISK.
  
  
  METHODS
  Fourteen corneal swabbing samples, from active diseased eyes of patients with clinical diagnosis of ISK, were submitted to Herpchek and PCR for the identification of HSV antigens and genome.
  
  
  RESULTS
  All ISK samples were negative by both techniques.
  
  
  CONCLUSIONS
  It was not possible to identify HSV antigens nor their genome by the methodology used. It is likely that, they can't be detected in corneal superficial layers or probably there is no viral replication at this stage of the disease, so antiviral therapy should be reconsidered.
authors.affiliation: Departamento de Oftalmología, Facultad de Medicina, Universidad de Chile. mmartine@machi.med.uchile.cl
authors.forename: C
authors.initials: C
authors.lastname: Vinagre
authors.forename: M J
authors.initials: MJ
authors.lastname: Martínez
authors.forename: M
authors.initials: M
authors.lastname: Vogel
authors.forename: L
authors.initials: L
authors.lastname: Traipe
authors.forename: J
authors.initials: J
authors.lastname: Stoppel
authors.forename: O
authors.initials: O
authors.lastname: Squella
authors.forename: M
authors.initials: M
authors.lastname: Srur
authors.forename: R
authors.initials: R
authors.lastname: Charlín
mesh_terms: D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D000956:Antigens, Viral / Q000032:analysis; D002648:Child; D002675:Child, Preschool; D002908:Chronic Disease; D003319:Corneal Stroma / Q000821:virology; D005260:Female; D016679:Genome, Viral; D006801:Humans; D016849:Keratitis, Herpetic / Q000821:virology*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012720:Severity of Illness Index; D018139:Simplexvirus / Q000235:genetics* / Q000276:immunology*
chemical_list: D000956:Antigens, Viral