Cellular localization of group IIA phospholipase A2 in rats.
It has been known that group II phospholipase A2 (PLA2) mRNA and protein are present in the homogenates of the spleen, lung, liver, and kidney in normal rats, but the cellular origin of this enzyme has not been yet identified. At present, five subtypes of group II PLA2 have been identified in mammals. Antibodies or mRNA probes previously used for detecting group II PLA2 need to be evaluated to identify the subtypes of group II PLA2. In this study we tried to identify group IIA PLA2-producing cells in normal rat tissues by in situ hybridization (ISH) using an almost full-length RNA probe for rat group IIA enzyme. Group IIA PLA2 mRNA was detected in megakaryocytes in the spleen and Paneth cells in the intestine by ISH. These cells were also immunopositive for an antibody raised against group IIA PLA(2) isolated from rat platelets. Group IIA PLA2 mRNA-positive cells were not detected in lung, liver, kidney, and pancreas. Under normal conditions, group IIA PLA2-producing cells are splenic megakaryocytes and intestinal Paneth cells in rats.
Yoshikawa
T
T
Department of Internal Medicine II, Nagoya University School of Medicine, Nagoya, Japan.
Naruse
S
S
Kitagawa
M
M
Ishiguro
H
H
Nagahama
M
M
Yasuda
E
E
Semba
R
R
Tanaka
M
M
Nomura
K
K
Hayakawa
T
T
D000818:Animals; D054501:Group II Phospholipases A2; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007583:Jejunum / Q000201:enzymology; D008297:Male; D008533:Megakaryocytes / Q000201:enzymology; D019879:Paneth Cells / Q000201:enzymology; D010741:Phospholipases A / Q000302:isolation & purification*; D054467:Phospholipases A2; D015347:RNA Probes; D051381:Rats; D017208:Rats, Wistar; D013154:Spleen / Q000201:enzymology; D014018:Tissue Distribution
D015347:RNA Probes; D010741:Phospholipases A; D054501:Group II Phospholipases A2; D054467:Phospholipases A2