title: Inactivation of catalase and superoxide dismutase by singlet oxygen derived from photoactivated dye.
abstract: Both superoxide dismutase (SOD) and catalase are key enzymes in the antioxidant system of the cells that work to maintain low steady-state concentrations of the reactive oxygen species. When exposed to a singlet oxygen-producing system composed of dye, such as methylene blue or rose bengal, and visible light both SOD and catalase were susceptible to oxidative modification and damage as indicated by the loss of activity, fragmentation and aggregation of peptide as well as by the formation of carbonyl groups. Histidine, a powerful quenching agent for singlet oxygen, and the polyamines, such as spermine and spermidine, were effective at protecting the activity loss mediated by illuminated dye, whereas spin traps were only mildly effective. The structural alterations of modified enzymes were indicated by the increase in susceptibility to proteases, the change in absorption spectra and in fluorescence spectra. The singlet oxygen-mediated damage to SOD and catalase may result in the perturbation of cellular antioxidant defense mechanisms and subsequently lead to a pro-oxidant condition.
authors.affiliation: Department of Biochemistry, College of Natural Sciences, Kyungpook National University, 702-701, Taegu, South Korea.
authors.forename: S Y
authors.initials: SY
authors.lastname: Kim
authors.forename: O J
authors.initials: OJ
authors.lastname: Kwon
authors.forename: J W
authors.initials: JW
authors.lastname: Park
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001386:Azides / Q000494:pharmacology; D002374:Catalase / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D002417:Cattle; D002614:Chelating Agents / Q000494:pharmacology; D002918:Chymotrypsin / Q000494:pharmacology; D004396:Coloring Agents / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases / Q000378:metabolism; D004789:Enzyme Activation; D004791:Enzyme Inhibitors / Q000494:pharmacology; D018384:Oxidative Stress; D010100:Oxygen / Q000378:metabolism*; D020539:Sequence Analysis, Protein; D013053:Spectrophotometry; D019097:Spin Trapping; D013482:Superoxide Dismutase / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D013997:Time Factors; D014357:Trypsin / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D001386:Azides; D002614:Chelating Agents; D004396:Coloring Agents; D004791:Enzyme Inhibitors; D002374:Catalase; D013482:Superoxide Dismutase; D010450:Endopeptidases; D002918:Chymotrypsin; D014357:Trypsin; D010100:Oxygen