Decreased susceptibility of Th1 cells to infection with primary HIV-1 isolates is associated with lower expression of CXCR4 in these cells.
The HIV-1 X4-isolates replicate more efficiently in Th2 than in Th1 cells, isolated from peripheral blood lymphocytes of HIV-1 seronegative individuals. The semiquantitative RT-PCR revealed higher level of transcripts of the HIV-1 co-receptor CXCR4 in Th2 cells than in Th1 cells which might explain better replication of HIV-1 X4-isolates in these cells. Lower expression of CXCR4 in Th1 cells render these cells less susceptible to infection with HIV-1.
Jagodzinski
P P
PP
Department of Biochemistry and Molecular Biology, University of Medical Sciences, Poznan, Poland. pjagodzi@eucalyptus.usoms.poznan.pl
Trzeciak
W H
WH
D002469:Cell Separation; D002999:Clone Cells; D015658:HIV Infections / Q000235:genetics / Q000378:metabolism* / Q000821:virology*; D015497:HIV-1; D006801:Humans; D066298:In Vitro Techniques; D012367:RNA, Viral / Q000096:biosynthesis; D019718:Receptors, CXCR4 / Q000096:biosynthesis* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018417:Th1 Cells / Q000378:metabolism* / Q000821:virology*; D018418:Th2 Cells / Q000378:metabolism / Q000821:virology
D012367:RNA, Viral; D019718:Receptors, CXCR4