title: NADPH and NADH serve as electron donor for the superoxide-generating enzyme in tilapia (Oreochromis niloticus) neutrophils.
abstract: NADPH oxidase has been identified as the superoxide-generating enzyme in fish neutrophils. To clarify the electron-donating ability of this enzyme, we examine the requirement of NADPH as the electron donor in superoxide generation in tilapia (Oreochromis niloticus) neutrophils using CLA-dependent chemiluminescence (CL). Phorbol ester-induced CL responses were terminated upon the addition of a detergent, Renex-30. The addition of graded amounts of NADPH or NADH restored the CL in a dose-dependent manner. The restoration of CL was completely eliminated by superoxide dismutase, suggesting that the restored CL was due to superoxide generation. NADPH tended to have a greater effect than NADH on the CL responses of tilapia neutrophils.
authors.affiliation: United Graduate School of Agricultural Science, Kagoshima University, Korimoto 1-21-24, 890-0065, Kagosima, Japan. si-bashi@brs.nihon-u.ac.jp
authors.forename: T
authors.initials: T
authors.lastname: Shiibashi
authors.forename: T
authors.initials: T
authors.lastname: Iida
mesh_terms: D000818:Animals; D003902:Detergents / Q000494:pharmacology; D004583:Electrons; D004798:Enzymes / Q000378:metabolism; D009243:NAD / Q000378:metabolism* / Q000494:pharmacology; D009249:NADP / Q000378:metabolism* / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D011092:Polyethylene Glycols / Q000494:pharmacology; D011719:Pyrazines; D013481:Superoxides / Q000378:metabolism*; D017210:Tilapia
chemical_list: D003902:Detergents; D004798:Enzymes; D011719:Pyrazines; D009243:NAD; C048193:2-methyl-6-phenyl-3,7-dihydroimidazo(1,2-a)pyrazin-3-one; D013481:Superoxides; C007590:emulphogene BC 720; D011092:Polyethylene Glycols; D009249:NADP