CCK mRNA expression in neuroendocrine CRH neurons is increased in rats subjected to an immune challenge.
The expression of cholecystokinin (CCK) mRNA in neuroendocrine corticotropin-releasing hormone (CRH) neurons of the hypothalamic paraventricular nucleus (PVN) of male rats was examined 8 h following an acute immune challenge by intraperitoneal lipopolysaccharide (LPS, 250 microg/kg). Both quantitative, macroautoradiographic, single-label radioactive in situ hybridization histochemistry (ISHH) and qualitative dual-label ISHH were performed. Compared to controls, LPS-injected rats displayed increased (185%) parvicellular CCK mRNA expression levels, occurring in a majority (70%) of CRH neurons as revealed by dual-label ISHH.
Juaneda
C
C
Laboratoire de Neurocytochimie Fonctionnelle, Université Bordeaux I, EA 2972, avenue des Facultés, 33405 cedex, Talence, France.
Lafon-Dubourg
P
P
Ciofi
P
P
Sarrieau
A
A
Wenger
T
T
Tramu
G
G
Corio
M
M
D000818:Animals; D002766:Cholecystokinin / Q000235:genetics*; D003346:Corticotropin-Releasing Hormone / Q000378:metabolism*; D007030:Hypothalamo-Hypophyseal System / Q000276:immunology / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D015213:Neuroimmunomodulation / Q000187:drug effects / Q000502:physiology; D009474:Neurons / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D010286:Paraventricular Hypothalamic Nucleus / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D010913:Pituitary-Adrenal System / Q000276:immunology / Q000378:metabolism*; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013312:Stress, Physiological / Q000378:metabolism / Q000503:physiopathology
D008070:Lipopolysaccharides; D012333:RNA, Messenger; D002766:Cholecystokinin; D003346:Corticotropin-Releasing Hormone