Analysis of the activity of promoters from two photosynthesis-related genes psaF and petH of spinach in a monocot plant, rice.
The subunit III of photosystem I and ferredoxin-NADP(+)-oxidoreductase are encoded by nuclear genes, namely psaF and petH. The activity of their promoters from spinach has been evaluated in transgenic tobacco earlier. Evaluation of the activity of these Dicotyledoneae-specific promoters has been carried out in a monocot system (i.e. rice) by transient gene expression system, based on electroporation-mediated gene delivery into protoplasts from leaves and roots. It has been found that various promoter deletions show higher activity in leaf protoplasts and elements for quantitative response are widely distributed. Transgenic rice has also been produced with a petH promoter and gus reporter gene construct. Although petH promoter is a weak promoter in comparison to the 35S promoter, it expresses well in green tissues and could be useful for plant genetic engineering.
Mohanty
A
A
Centre for Plant Molecular Biology and Department of Plant Molecular Biology, University of Delhi, South Campus, Benito Juarez Road, New Delhi 110021, India.
Grover
M
M
Chaudhury
A
A
Rizwan-ul-Haq
Q
Q
Sharma
A K
AK
Maheshwari
S C
SC
Tyagi
A K
AK
D001426:Bacterial Proteins / Q000235:genetics*; D005287:Ferredoxin-NADP Reductase / Q000235:genetics*; D005420:Flavoproteins; D017930:Genes, Reporter; D008565:Membrane Proteins / Q000235:genetics*; D012275:Oryza / Q000235:genetics*; D010788:Photosynthesis / Q000235:genetics*; D045331:Photosystem I Protein Complex; D010940:Plant Proteins; D030821:Plants, Genetically Modified / Q000235:genetics; D011401:Promoter Regions, Genetic; D018724:Spinacia oleracea / Q000235:genetics*
D001426:Bacterial Proteins; D005420:Flavoproteins; D008565:Membrane Proteins; D045331:Photosystem I Protein Complex; D010940:Plant Proteins; D005287:Ferredoxin-NADP Reductase; C082502:petH protein, Bacteria