[Diagnostic value of human cytomegalovirus late-mRNA detection for intrauterine active human cytomegalovirus infection].
OBJECTIVE
To study diagnostic value of late-mRNA detection for intrauterine active human cytomegalovirus (HCMV) infection.


METHODS
The HCMV late-mRNA in peripheral blood of the pregnant women with HCMV-IgM positive and their fetal appendages (such as chorionic villi, amniotic fluid, umbilical blood and placenta) were detected by reverse transcription polymerase chain reaction(RT-PCR).


RESULTS
The late-mRNA was detected in 23 out of 42 HCMV-IgM positive pregnant cases, with the 54.3% of corresponding rate between the results of HCMV-IgM and HCMV late-mRNA. 7 samples out of 13 fetal appendages from m-RNA positive mothers were late-mRNA positive, while in 12 mothers with late-mRNA negative group only one fetal sample was late-mRNA positive, Between two groups there was significant difference in mother-fetus transmission rate.


CONCLUSIONS
The positive result of HCMV-IgM can not accurately reflect HCMV activity at the time being detected, which is closely related to the mother-fetus transmission rate. As a symbol of active HCMV infection, late-mRNA can not only reflect the mother-fetus transmission rate during active HCMV infection correctly, but also provide some information about activity of the HCMV in fetal tissue, It also has a great value in prediction for prognosis of infectious fetuses.
Jiang
H
H
Hunan Reproductive Engineering, Hunan Medical University, Changsha 410078.
Wen
L
L
Ling
X
X
D003587:Cytomegalovirus / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000635:transmission*; D005260:Female; D005315:Fetal Diseases / Q000175:diagnosis; D006801:Humans; D007231:Infant, Newborn; D018445:Infectious Disease Transmission, Vertical; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000821:virology*; D011296:Prenatal Diagnosis / Q000379:methods*; D012333:RNA, Messenger / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D012333:RNA, Messenger; D012367:RNA, Viral