title: Volume-load capacity in fast-gradient liquid chromatography effect of sample solvent composition and injection volume on chromatographic performance.
abstract: We studied the effects of sample solvent composition and injection volumes on the chromatographic performance of ODS-bonded silica columns under fast-gradient running conditions. Chromatographic performance is compromised as a function of both sample injection volume and sample solvent strength, with earlier-eluting analytes being much more affected than later-eluting ones. In general, when injecting samples dissolved in a strong solvent, performance was improved by diluting the strong injection solvent and injecting a proportionally larger volume. Volume loading capacity can be increased by using a longer column, or by using a column of equivalent length, but with a larger inner diameter. Data also suggest that sample solvent strength, not viscosity, is responsible for the noted effects.
authors.affiliation: Phenomenex Inc., Torrance, CA 90501, USA. jeffl@phenomenex.com
authors.forename: J
authors.initials: J
authors.lastname: Layne
authors.forename: T
authors.initials: T
authors.lastname: Farcas
authors.forename: I
authors.initials: I
authors.lastname: Rustamov
authors.forename: F
authors.initials: F
authors.lastname: Ahmed
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D012997:Solvents / Q000737:chemistry*
chemical_list: D012997:Solvents