[Mineral salt-dependent inhibition of light emission from the luminescent microorganism Escherichia coli Z9051].
The influence of some mineral salts on the recombinant strain Escherichia coli Z9051 was investigated. It was shown that the composition (NaCl, Na2SO4, MgCl2 and MgSO4) and concentration (5 and 10%) of the salts substantially affect the expression of genes for the luminescence system of light-emitting bacteria cloned in the plasmid under the control of the lac-promoter. In some cases, the luminescence level of the microorganism in the presence of salts was similar to the luminescence level under catabolite repression by glucose, the more strong influence of the salts exceeding the effect of catabolite repression. The possibility of adaptation of the genetically modified microorganism to the salinity factor is discussed.
Boiandin
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Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Akademgorodok, 660036 Russia.
Popova
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LIu
D002712:Chlorides / Q000494:pharmacology*; D003470:Culture Media; D003864:Depression, Chemical; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D049449:Luminescence; D008274:Magnesium / Q000494:pharmacology*; D010776:Photobacterium / Q000235:genetics; D012492:Salts; D012964:Sodium / Q000494:pharmacology*; D013431:Sulfates / Q000494:pharmacology*
D002712:Chlorides; D003470:Culture Media; D012492:Salts; D013431:Sulfates; D012964:Sodium; D008274:Magnesium