title: Invasive pneumococcal infections in children infected with HIV are not associated with splenic dysfunction.
abstract: OBJECTIVE
  Children infected with HIV-1 are more likely to acquire infections associated with the encapsulated bacterial pathogens of childhood than their non-HIV-infected peers. The goal of the current study was to determine what proportion of community-acquired, invasive pneumococcal disease in HIV-infected children could be attributed to splenic dysfunction, as measured by enumerating the number of pocked red blood cells (RBCs) in peripheral blood.
  
  
  METHODS
  Splenic reticuloendothelial function was assessed semiquantitatively by examining the morphology of the RBCs of 84 children born to HIV-infected mothers using phase interference microscopy. Surveillance of medical records, and a review of the Yale-New Haven Hospital Clinical Microbiology computerized database, revealed that all of the bacterial cultures of blood and cerebrospinal fluid from these patients were positive.
  
  
  RESULTS
  Of the 84 children assessed, 70 were infected with HIV (median age 66 months) and 14 were uninfected seroreverters (controls). Sixty-one of the 70 HIV-infected children met the CDC criteria for moderate or severe immunodeficiency and/or moderate or severe symptomatic conditions. Seventeen of the 70 HIV-infected children experienced 23 invasive bacterial infections. Streptococcus pneumoniae was responsible for all 23 infections. The median age at the time of first infection among these 17 subjects was 20 months (range, 10-58 months). There were no episodes of invasive bacterial infections in the remaining 53 HIV-infected children nor among the 14 controls. All 84 children studied, including those with invasive pneumococcal disease, had normal proportions (i.e., < 2%) of pocked erythrocytes in peripheral blood.
  
  
  CONCLUSIONS
  Splenic dysfunction, as measured by the pocked RBC count, does not account for the increased occurrence of invasive pneumococcal disease found in children infected with HIV.
authors.affiliation: Department of Pediatrics, Yale University School of Medicine, New Haven, Connecticut, USA.
authors.forename: W A
authors.initials: WA
authors.lastname: Andiman
authors.forename: J
authors.initials: J
authors.lastname: Simpson
authors.forename: C
authors.initials: C
authors.lastname: Holtkamp
authors.forename: H A
authors.initials: HA
authors.lastname: Pearson
mesh_terms: D017088:AIDS-Related Opportunistic Infections / Q000150:complications*; D016470:Bacteremia / Q000150:complications*; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D017714:Community-Acquired Infections / Q000150:complications*; D004906:Erythrocyte Count; D015497:HIV-1; D006801:Humans; D007223:Infant; D011008:Pneumococcal Infections / Q000150:complications*; D013158:Splenic Diseases / Q000097:blood / Q000821:virology*