Identification of a new water channel (Rp-MIP) in the Malpighian tubules of the insect Rhodnius prolixus.
Malpighian tubules (MT) of Rhodnius prolixus transport fluid at very high rates. To identify whether aquaporins (AQPs) are present in the MT of R. prolixus, total ribonucleic acid (RNA) was isolated from MT and used in a reverse transcription, polymerase chain reaction (RT-PCR), with two degenerate primers to highly conserved regions of the members of the AQPs family. A deoxyribonucleic acid (DNA) fragment of 370 bp was amplified; its sequence revealed a novel protein, representing a new member of the major intrinsic protein (MIP) family. The complementary DNA (cDNA) sequence of this new MIP protein was cloned by using RNA from MT and the rapid amplification of cDNA ends (RACE) technique. The cDNA had 1133 bp and the largest open reading frame coded for a protein of 286 amino acids, named R. prolixus major intrinsic protein (Rp-MIP). The hydrophobicity profile of the amino acid sequence predicts six transmembrane domains. Northern blot analysis of MT RNA showed a single transcript of about 1-1.3 kb for Rp-MIP. RT-PCR of single isolated MT and in situ hybridization analysis showed Rp-MIP transcripts in both proximal and distal segments. Expression of Rp-MIP in Xenopus laevis oocytes doubled the osmotic water permeability Pf, indicating that Rp-MIP may function as an aquaporin protein in the MT of the insect and thus may participate in urine formation in R. prolixus.
Echevarría
M
M
Departamento de Fisiología y Laboratorio de Investigaciones Biomédicas, Universidad de Sevilla y Hospital Universitario Virgen del Rocío, Edificio de Laboratorios, Av. Manuel Siurot s/n, 41013 Sevilla, Spain. irusta@cica.es
Ramírez-Lorca
R
R
Hernández
C S
CS
Gutiérrez
A
A
Méndez-Ferrer
S
S
González
E
E
Toledo-Aral
J J
JJ
Ilundáin
A A
AA
Whittembury
G
G
D000595:Amino Acid Sequence; D000818:Animals; D020346:Aquaporins / Q000235:genetics; D001483:Base Sequence; D002462:Cell Membrane / Q000737:chemistry; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis / Q000737:chemistry; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D017403:In Situ Hybridization; D019476:Insect Proteins / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D008317:Malpighian Tubules / Q000737:chemistry*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D012313:RNA / Q000302:isolation & purification; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012239:Rhodnius; D016415:Sequence Alignment
D020346:Aquaporins; D018076:DNA, Complementary; D019476:Insect Proteins; D012333:RNA, Messenger; C437971:Rp-MIP protein, Rhodnius; D012313:RNA; D004247:DNA