title: [Distribution of tryptophan groups in molecules of troponin T, troponin I-troponin T complex, and alpha-actinin].
abstract: The method of fluorescence quenching was used to experimentally determine the distribution of tryptophan residues in molecules of troponin T, troponin T-troponin I complexes, and alpha-actinin. Iodide and cesium ions, and acrylamide were used as quenchers. It was shown that cesium ions decrease the fluorescence intensity of troponin T and its complex with troponin I by the mode of dynamic quenching. For alpha-actinin such a dynamic quencher is anionic iodide. By using the modified Stern-Volmer equation, the quenching was found to be about 90% of total fluorescence intensity for troponin T, approximately 70% for the troponin T-troponin I complexes, and 20% for alpha-actinin. The penetration of cesium ions to tryptophan 206 (tryptophan 204) in the troponin T-troponin I complex is hindered, probably due to the participation of this tryptophan in the formation of bonds between troponin subunits.
authors.affiliation: Institute of Molecular Biology and Biololical Physics, Georgian Academy of Sciences, ul. Gotua 12, Tbilisi, 380060 Georgia.
authors.forename: A G
authors.initials: AG
authors.lastname: Gvritishvili
authors.forename: S O
authors.initials: SO
authors.lastname: Simonishvili
authors.forename: N I
authors.initials: NI
authors.lastname: Butkhuzi
authors.forename: K Sh
authors.initials: KSh
authors.lastname: Kuridze
authors.forename: M Sh
authors.initials: MSh
authors.lastname: Simonidze
authors.forename: E V
authors.initials: EV
authors.lastname: But
authors.forename: M M
authors.initials: MM
authors.lastname: Zaalishvili
mesh_terms: D000185:Actinin / Q000737:chemistry*; D000818:Animals; D018482:Muscle, Skeletal / Q000737:chemistry; D011817:Rabbits; D013050:Spectrometry, Fluorescence; D019210:Troponin I / Q000737:chemistry*; D020107:Troponin T / Q000737:chemistry*; D014364:Tryptophan / Q000737:chemistry*
chemical_list: D019210:Troponin I; D020107:Troponin T; D000185:Actinin; D014364:Tryptophan