Comparative study using amplified fragment length polymorphism fingerprinting, PCR genotyping, and phenotyping to differentiate Campylobacter fetus strains isolated from animals.
A collection of Campylobacter fetus strains, including both C. fetus subsp. fetus and C. fetus subsp. venerealis, were phenotypically identified to the subspecies level and genotypically typed by PCR and amplified fragment length polymorphism (AFLP) analysis. Phenotypic subspecies determination methods were unreliable. Genotyping of the strains by PCR and AFLP showed a clear discrimination between the two subspecies.
Wagenaar
J A
JA
Institute for Animal Science and Health, 8200 AB Lelystad, The Netherlands. j.a.wagenaar@id.wag-ur.nl
van Bergen
M A
MA
Newell
D G
DG
Grogono-Thomas
R
R
Duim
B
B
D000818:Animals; D015373:Bacterial Typing Techniques; D002168:Campylobacter fetus / Q000145:classification* / Q000235:genetics / Q000378:metabolism; D016172:DNA Fingerprinting / Q000379:methods*; D005838:Genotype; D010641:Phenotype; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic