title: Longitudinal analysis of CD8(+) T-cell phenotype and IL-7, IL-15 and IL-16 mRNA expression in different tissues during primary simian immunodeficiency virus infection.
abstract: Infection of macaques with pathogenic isolates of simian immunodeficiency virus (SIV) represents a useful model of HIV infection that offers the unique opportunity to investigate the very early modifications that affect CD8(+) T-lymphocyte subsets and related cytokines during lentiviral infection. Herein, three cynomolgus macaques were inoculated intravenously with a pathogenic isolate of SIVmac 251. In fresh isolated mononuclear cells from blood, lymph node and bronchoalveolar lavage, we analyzed changes in the phenotype of CD8(+) T cells and we used reverse transcription-PCR to monitor the expression of IL-7, IL-15 and IL-16 mRNA. We demonstrated that an expansion of CD8(+)CD28(-) T cells occurs from the third week of infection on in the peripheral blood and in the lung, whereas CD8(+)CD28(+) T cells expand in the lymph nodes. Concomitantly, we evidenced mRNA modulations in IL-16, IL-15 and IL-7 expression in the three compartments studied. The containment of systemic viral replication was associated with an overexpression of IL-16 mRNA in the lung and in the peripheral blood. Given the immunomodulatory properties of IL-15 and IL-7 and the potential antiviral ability of IL-16, these perturbations could have important implications in early viral dissemination and HIV immunopathogenesis.
authors.affiliation: CEA, service de neurovirologie, DSV/DRM, CRSSA, institut Paris-Sud sur les cytokines, Fontenay-aux-Roses, France.
authors.forename: P
authors.initials: P
authors.lastname: Caufour
authors.forename: R
authors.initials: R
authors.lastname: Le Grand
authors.forename: A
authors.initials: A
authors.lastname: Chéret
authors.forename: O
authors.initials: O
authors.lastname: Neildez
authors.forename: H
authors.initials: H
authors.lastname: Thiébot
authors.forename: F
authors.initials: F
authors.lastname: Théodoro
authors.forename: B
authors.initials: B
authors.lastname: Boson
authors.forename: B
authors.initials: B
authors.lastname: Vaslin
authors.forename: A
authors.initials: A
authors.lastname: Venet
authors.forename: D
authors.initials: D
authors.lastname: Dormont
mesh_terms: D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology; D018106:CD28 Antigens / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D004195:Disease Models, Animal; D019409:Interleukin-15 / Q000235:genetics; D019410:Interleukin-16 / Q000235:genetics; D015851:Interleukin-7 / Q000235:genetics; D007700:Kinetics; D008137:Longitudinal Studies; D008198:Lymph Nodes / Q000276:immunology; D018655:Lymphocyte Count; D008252:Macaca fascicularis; D010641:Phenotype; D012333:RNA, Messenger / Q000032:analysis; D012367:RNA, Viral / Q000097:blood; D016097:Simian Acquired Immunodeficiency Syndrome / Q000097:blood / Q000276:immunology* / Q000821:virology; D015302:Simian Immunodeficiency Virus / Q000235:genetics
chemical_list: D018106:CD28 Antigens; D019409:Interleukin-15; D019410:Interleukin-16; D015851:Interleukin-7; D012333:RNA, Messenger; D012367:RNA, Viral