title: Study of a GABAC receptor antagonist on sleep-waking behavior in rats.
abstract: BACKGROUND
  gamma-Aminobutyric acid (GABA) is the main inhibitory transmitter of the brain. The involvement of GABAA and GABAB receptors in sleep-waking processes is well established.
  
  
  OBJECTIVE
  This research studied the influence of GABAC receptors.
  
  
  METHODS
  The rats were randomly infused in the fourth ventricle with vehicle and 25, 50, and 100 micrograms (1,2,5,6,-tetrahydropyridine)-methylphosphinic acid (TPMPA), a specific antagonist of GABAC receptors.
  
  
  RESULTS
  Principally at 50 micrograms, the molecule induced an increase of waking from 44.7% to 61.7% (P < 0.003), which was the consequence of enhancement of both active and quiet wakefulness. Total slow wave sleep was decreased, particularly the slow-wave stage from 39% to 27.7% (P < 0.02). Paradoxical sleep was also decreased from 14.5% to 9.1% (P < 0.01).
  
  
  CONCLUSIONS
  GABAC receptors are also involved in sleep-waking regulation. Since the sensitivity of GABAC receptors to GABA is much higher than that of GABAA and GABAB receptors, GABAC receptor modulators could be potential medications acting at low doses with fewer side effects.
authors.affiliation: Laboratoire de Psychophysiologie, Faculté des Sciences, Université de Nice-Sophia Antipolis, 06108 Nice, France.
authors.forename: C
authors.initials: C
authors.lastname: Arnaud
authors.forename: P
authors.initials: P
authors.lastname: Gauthier
authors.forename: C
authors.initials: C
authors.lastname: Gottesmann
mesh_terms: D000818:Animals; D018756:GABA Antagonists / Q000494:pharmacology*; D007276:Injections, Intraventricular; D008297:Male; D010721:Phosphinic Acids / Q000494:pharmacology*; D011725:Pyridines / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D018079:Receptors, GABA / Q000502:physiology*; D012890:Sleep / Q000187:drug effects* / Q000502:physiology; D012895:Sleep, REM / Q000187:drug effects / Q000502:physiology; D014851:Wakefulness / Q000187:drug effects* / Q000502:physiology
chemical_list: C101879:(1,2,5,6-tetrahydropyridin-4-yl)methylphosphinic acid; D018756:GABA Antagonists; C087200:GABA-C receptor; D010721:Phosphinic Acids; D011725:Pyridines; D018079:Receptors, GABA