Replacement of the coat protein gene of plum pox potyvirus with that of zucchini yellow mosaic potyvirus: characterization of the hybrid potyvirus.
Infectious hybrid virus was generated by replacing part of the coat protein gene of plum pox potyvirus with that of the zucchini yellow mosaic potyvirus. This viable hybrid contains 84.5% of zucchini yellow mosaic potyvirus coat protein gene while the rest of the sequence was derived from plum pox potyvirus. Changing the coat protein gene between these two viruses had no effect on the experimental host range. Pathogenicity, stability and replication capacity of the hybrid virus were nearly identical to the parent viruses.
Tóbiás
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Plant Protection Institute, Hungarian Academy of Sciences, H-1515 P.O. Box 102, Budapest, Hungary.
Palkovics
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L
Tzekova
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L
Balázs
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E
D000595:Amino Acid Sequence; D019943:Amino Acid Substitution / Q000235:genetics*; D000818:Animals; D015153:Blotting, Western; D002213:Capsid / Q000235:genetics / Q000378:metabolism*; D036022:Capsid Proteins; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000821:virology; D010944:Plants / Q000821:virology*; D017801:Plum Pox Virus / Q000235:genetics / Q000472:pathogenicity* / Q000502:physiology*; D017800:Potyvirus / Q000235:genetics / Q000472:pathogenicity* / Q000502:physiology*; D013045:Species Specificity; D014675:Vegetables / Q000821:virology; D014764:Viral Proteins / Q000032:analysis; D014779:Virus Replication
D036022:Capsid Proteins; D014764:Viral Proteins; C086081:coat protein, Zucchini yellow mosaic virus