title: Pimprinine, an extracellular alkaloid produced by Streptomyces CDRIL-312: fermentation, isolation and pharmacological activity.
abstract: Pimprinine, an extracellular alkaloid has been isolated from the culture filtrate of Streptomyces CDRIL-312. Pimprinine was subsequently purified using silica gel column chromatography and also by preparatory thin layer chromatography. Some physicochemical properties, antimicrobial activities (in-vitro) and pharmacological activities of pimprinine were studied. Pimprinine showed promising anticonvulsant activity in both minimum and maximum electric seizure threshold test in mice. Its anticonvulsant activity is very much comparable to that of phenyl hydantion sodium. Pimprinine also inhibited effectively tremorine-induced tremors and analgesia in mice.
authors.affiliation: Laboratory of Industrial Microbiology and Fermentation, Antibiotics Research Centre, Hindustan Antibiotics Limited, Pimpri, 411018, Pune, India. kmkcp@bom3.vsnl.net.in
authors.forename: S R
authors.initials: SR
authors.lastname: Naik
authors.forename: J
authors.initials: J
authors.lastname: Harindran
authors.forename: A B
authors.initials: AB
authors.lastname: Varde
mesh_terms: D000818:Animals; D000927:Anticonvulsants / Q000494:pharmacology*; D001709:Biotechnology / Q000379:methods; D002855:Chromatography, Thin Layer / Q000379:methods; D004305:Dose-Response Relationship, Drug; D005285:Fermentation; D007928:Lethal Dose 50; D051379:Mice; D008815:Mice, Inbred Strains; D008826:Microbial Sensitivity Tests; D010080:Oxazoles / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology*; D010672:Phenytoin / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology*; D012131:Respiratory Insufficiency / Q000139:chemically induced; D012640:Seizures / Q000188:drug therapy; D013302:Streptomyces / Q000378:metabolism*; D018675:Toxicity Tests; D014202:Tremor / Q000139:chemically induced / Q000188:drug therapy; D014203:Tremorine / Q000633:toxicity
chemical_list: D000927:Anticonvulsants; D010080:Oxazoles; C008912:pimprinine; D010672:Phenytoin; D014203:Tremorine