Adhesion of alpha5beta1 receptors to biomimetic substrates constructed from peptide amphiphiles.
Biomimetic membrane surfaces functionalized with fragments of the extracellular matrix protein, fibronectin, are constructed from mixtures of peptide and polyethylene glycol (PEG) amphiphiles. Peptides from the primary binding loop, GRGDSP, were used in conjunction with the synergy site peptide, PHSRN, in the III(9-10) sites of human fibronectin. These peptides were attached to dialkyl lipid tails to form peptide amphiphiles. PEG amphiphiles were mixed in the layer to minimize non-specific adhesion in the background. GRGDSP and PEG amphiphiles or GRGDSP, PHSRN, and PEG amphiphiles were mixed in various ratios and deposited on solid substrates from the air-water interface using Langmuir-Blodgett techniques. In this method, peptide composition, density, and presentation could be controlled accurately. The effectiveness of these substrates to mimic native fibronectin is evaluated by their ability to generate adhesive forces when they are in contact with purified activated alpha5beta1 integrin receptors that are immobilized on an opposing surface. Adhesion is measured using a contact mechanical approach (JKR experiment). The effects of membrane composition, density, temperature, and peptide conformation on adhesion to activated integrins in this simulated cell adhesion setup were determined. Addition of the synergy site, PHSRN, was found to increase adhesion of alpha5beta1, to biomimetic substrates markedly. Increased peptide mobility (due to increased experimental temperature) increased integrin adhesion markedly at low peptide concentrations. A balance between peptide density and steric accessibility of the receptor binding face to alpha5beta1 integrin was required for highest adhesion.
Dillo
A K
AK
Department of Chemical Engineering and Materials Science, University Minnesota, Minneapolis 55455, USA.
Ochsenhirt
S E
SE
McCarthy
J B
JB
Fields
G B
GB
Tirrell
M
M
D000595:Amino Acid Sequence; D001665:Binding Sites; D001672:Biocompatible Materials / Q000737:chemistry*; D006020:Glycopeptides / Q000737:chemistry*; D007700:Kinetics; D008958:Models, Molecular; D009842:Oligopeptides / Q000737:chemistry*; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry*; D011312:Pressure; D011487:Protein Conformation; D017469:Receptors, Fibronectin / Q000737:chemistry*; D013499:Surface Properties; D013816:Thermodynamics
D001672:Biocompatible Materials; D006020:Glycopeptides; D009842:Oligopeptides; D010446:Peptide Fragments; D017469:Receptors, Fibronectin; D011092:Polyethylene Glycols