Marked neointimal lipoprotein lipase increase in distinct models of proclivity to atherosclerosis: a feature independent of endothelial layer integrity.
Lipoprotein lipase (LPL) in the arterial wall has been proposed to enhance the retention of apoB-containing lipoproteins, an early event in atherosclerosis. As the neointima is considered the primary site of lipid accumulation in atherogenesis, the arterial expression and location of LPL was investigated in distinct experimental models of neointimal formation in normolipidemic rabbits and rats. Neointima elicited by balloon aortic denudation or raised beneath an anatomically intact endothelial layer by placing a silastic collar around the common carotid artery, both showed a striking LPL immunostaining that mostly co-localized with neointimal smooth muscle cells. Besides, increased LPL protein and mRNA in deendothelialized aortas was demonstrated by Western and Northern blot analysis, respectively, suggesting an enhanced expression of LPL in injured arteries. It was concluded that LPL is increased in neointima developed in either denuded vessels or arteries with a preserved endothelium, a finding which suggests that LPL abundance may be an attribute of the neointima, whatever the stimulus that promotes its formation. On the basis of former evidence concerning the role of LPL in lipid retention, this study provides a possible explanation for the injury-induced vessel susceptibility to atherosclerosis, and the particular proneness of the neointimal layer to lipid accretion.
Werba
J P
JP
Lipid and Atherosclerosis Research Laboratory, Department of Basic Sciences of Pathology, Proyect Id. 008-99, Favaloro University, Solis 453 (1078), Buenos Aires, Argentina. jpwerba@favaloro.edu.ar
Martinez
V
V
Abulafia
D P
DP
Levy
R
R
Magariños
G
G
Rey
R H
RH
Baetta
R
R
Soma
M R
MR
Añel
A D
AD
Cuniberti
L A
LA
D000818:Animals; D001011:Aorta / Q000201:enzymology* / Q000378:metabolism; D001013:Aorta, Thoracic / Q000473:pathology; D001161:Arteriosclerosis / Q000209:etiology*; D002339:Carotid Arteries / Q000201:enzymology* / Q000378:metabolism / Q000473:pathology; D004730:Endothelium, Vascular / Q000502:physiology; D008071:Lipoprotein Lipase / Q000235:genetics / Q000378:metabolism*; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar; D012307:Risk Factors; D017539:Tunica Intima / Q000201:enzymology*
D012333:RNA, Messenger; D008071:Lipoprotein Lipase