Perichondrium-wrapped collagenous matrices to induce chondroneogenesis: an in vitro study.
OBJECTIVE
To develop a model for cartilage regeneration in vitro, to be used for cartilage reconstruction in vivo.


METHODS
Collagenous matrices were wrapped in a perichondrium layer. The matrices served as carriers to allow migration of cells from the perichondrium into the matrix. Culture conditions stimulated cell growth and proliferation.


RESULTS
After 4 weeks of culturing, microscopic examination showed an increase of cell layers around the matrices but also of cells migrated into porous matrices. Immunohistochemical staining of these cultured cells expressed type II collagen intracellularly.


CONCLUSIONS
This model seems appropriate to culture mucoperichondrial explants in combination with collagenous matrices. Cells migrate into the pores of the matrix, survive, and synthesize matrix components. Actual formation of cartilage has not been shown to occur. Adding growth factors to this model may influence induction of this activity.
van Lange
J W
JW
Department of Otorhinolaryngology, Academical Medical Center, University of Amsterdam, PO Box 22660, 1100 DD Amsterdam, The Netherlands. j.w.vanlange@amc.uva.nl
de Roo
K
K
Middelkoop
E
E
van den Bos
T
T
Everts
V
V
Nolst Trenité
G J
GJ
D000818:Animals; D021522:Bioartificial Organs; D001672:Biocompatible Materials; D002356:Cartilage / Q000166:cytology* / Q000378:metabolism; D002478:Cells, Cultured; D020219:Chondrogenesis; D003094:Collagen; D003239:Connective Tissue Cells / Q000166:cytology* / Q000378:metabolism; D005260:Female; D007150:Immunohistochemistry; D011392:Proline / Q000378:metabolism; D011817:Rabbits
D001672:Biocompatible Materials; D003094:Collagen; D011392:Proline