Microbial pathway engineering for industrial processes: evolution, combinatorial biosynthesis and rational design.
Microbial pathway engineering has made significant progress in multiple areas. Many examples of successful pathway engineering for specialty and fine chemicals have been reported in the past two years. Novel carotenoids and polyketides have been synthesized using molecular evolution and combinatorial strategies. In addition, rational design approaches based on metabolic control have been reported to increase metabolic flux to specific products. Experimental and computational tools have been developed to aid in design, reconstruction and analysis of non-native pathways. It is expected that a hybrid of evolutionary, combinatorial and rational design approaches will yield significant advances in the near future.
Rohlin
L
L
Department of Chemical Engineering, 405 Hilgard Avenue, University of California, Los Angeles, California 90095, USA.
Oh
M K
MK
Liao
J C
JC
D002338:Carotenoids / Q000378:metabolism; D007218:Industrial Microbiology / Q000379:methods / Q000639:trends; D008828:Microbiological Techniques; D011106:Polyisoprenyl Phosphates / Q000378:metabolism
D011106:Polyisoprenyl Phosphates; D002338:Carotenoids