title: Expression of a plant gene with sequence similarity to animal TGF-beta receptor interacting protein is regulated by brassinosteroids and required for normal plant development.
abstract: Brassinosteroids (BRs) regulate the expression of numerous genes associated with plant development, and require the activity of a Ser/Thr receptor kinase to realize their effects. In animals, the transforming growth factor-beta (TGF-beta) family of peptides acts via Ser/Thr receptor kinases to have a major impact on several pathways involved in animal development and adult homeostasis. TGF-beta receptor-interacting protein (TRIP-1) was previously shown by others to be an intracellular substrate of the TGF-beta type II receptor kinase which plays an important role in TGF-beta signaling. TRIP-1 is a WD-repeat protein that also has a dual role as an essential subunit of the eukaryotic translation initiation factor eIF3 in animals, yeast and plants, thereby revealing a putative link between a developmental signaling pathway and the control of protein translation. In yeast, expression of a TRIP-1 homolog has also been closely associated with cell proliferation and progression through the cell cycle. We report here the novel observation that transcript levels of TRIP-1 homologs in plants are regulated by BR treatment under a variety of conditions, and that transgenic plants expressing antisense TRIP-1 RNA exhibit a broad range of developmental defects, including some that resemble the phenotype of BR-deficient and -insensitive mutants. This correlative evidence suggests that a WD-domain protein with reported dual functions in vertebrates and fungi might mediate some of the molecular mechanisms underlying the regulation of plant growth and development by BRs.
authors.affiliation: Department of Horticultural Science, North Carolina State University, Raleigh, NC 27695-7609, USA.
authors.forename: J
authors.initials: J
authors.lastname: Jiang
authors.forename: S D
authors.initials: SD
authors.lastname: Clouse
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D016375:Antisense Elements (Genetics); D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D029681:Arabidopsis Proteins; D015152:Blotting, Northern; D060406:Brassinosteroids; D002777:Cholestanols / Q000378:metabolism*; D016384:Consensus Sequence; D039621:Eukaryotic Initiation Factor-3; D007887:Fabaceae / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D010937:Plant Growth Regulators / Q000378:metabolism*; D018514:Plant Structures / Q000378:metabolism; D030821:Plants, Genetically Modified; D010946:Plants, Medicinal; D010947:Plants, Toxic; D016133:Polymerase Chain Reaction; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000235:genetics* / Q000378:metabolism; D018125:Receptors, Transforming Growth Factor beta / Q000378:metabolism*; D020449:Repetitive Sequences, Amino Acid; D017386:Sequence Homology, Amino Acid; D013260:Steroids, Heterocyclic / Q000378:metabolism*; D014026:Nicotiana / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism
chemical_list: D016375:Antisense Elements (Genetics); D029681:Arabidopsis Proteins; D060406:Brassinosteroids; D002777:Cholestanols; D039621:Eukaryotic Initiation Factor-3; D010937:Plant Growth Regulators; D011506:Proteins; D018125:Receptors, Transforming Growth Factor beta; D013260:Steroids, Heterocyclic; C484317:TRIP-1 protein, Arabidopsis; C096013:EIF3I protein, human; C023623:brassinolide