Quality control of PCR primers used in multiplex STR amplification reactions.
Reliable amplification of short tandem repeat (STR) DNA markers with the polymerase chain reaction (PCR) is dependent on high quality PCR primers. The particular primer combinations and concentrations are especially important with multiplex amplification reactions where multiple STR loci are simultaneously copied. Commercially available kits are now widely used for STR amplification and subsequent DNA typing. We present here the use of high performance liquid chromatography (HPLC) and time-of-flight mass spectrometry (TOF-MS) methods for characterization of commercially available STR kits.
Butler
J M
JM
Biotechnology Division, National Institute of Standards and Technology, 100 Bureau Drive, Mail Stop 8311, Gaithersburg, MD 20899, USA. john.butler@nist.gov
Devaney
J M
JM
Marino
M A
MA
Vallone
P M
PM
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D017931:DNA Primers / Q000592:standards*; D016002:Discriminant Analysis; D005554:Forensic Medicine / Q000379:methods*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D018598:Minisatellite Repeats / Q000235:genetics*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011786:Quality Control; D013997:Time Factors
D017931:DNA Primers