title: A field evaluation of in situ biodegradation of trichloroethylene through methane injection.
abstract: A field study of biodegradation of trichloroethylene (TCE) through methane injection was conducted at the yard of a home in Japan. Methane was selected as the safest substrate for injection into groundwater. Methane, oxygen, nitrate, and phosphate were introduced into groundwater contaminated with 220 microg/L of TCE. After a week of biostimulation, methane concentrations gradually decreased below the detection limit. Methane oxidizing bacterial numbers increased from 10 to 10(4) cells/mL with methane consumptions. During methane injection. 10-20% of TCE removal was observed. The biotransformation yield was 3-13 mgTCE/gCH4 in this field test. After methane injections were stopped, TCE removal was not observed. These results indicated that bioremediation using methane was useful as a safe technology for a TCE-contaminated area near homes.
authors.affiliation: Central Research Laboratories, Organo Corporation, Toda, Japan. eguchi-m@organo.co.jp
authors.forename: M
authors.initials: M
authors.lastname: Eguchi
authors.forename: M
authors.initials: M
authors.lastname: Kitagawa
authors.forename: Y
authors.initials: Y
authors.lastname: Suzuki
authors.forename: M
authors.initials: M
authors.lastname: Nakamuara
authors.forename: T
authors.initials: T
authors.lastname: Kawai
authors.forename: K
authors.initials: K
authors.lastname: Okamura
authors.forename: S
authors.initials: S
authors.lastname: Sasaki
authors.forename: Y
authors.initials: Y
authors.lastname: Miyake
mesh_terms: D001673:Biodegradation, Environmental; D007564:Japan; D008697:Methane / Q000378:metabolism; D008772:Methylococcaceae / Q000378:metabolism; D012449:Safety; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000302:isolation & purification / Q000378:metabolism; D014241:Trichloroethylene / Q000302:isolation & purification / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D012989:Soil Pollutants; D014874:Water Pollutants, Chemical; D014241:Trichloroethylene; D008697:Methane