title: A BAC-based STS-content map spanning a 35-Mb region of human chromosome 1p35-p36.
abstract: We have devised a mapping method for rapid assembly and ordering of bacterial artificial chromosome (BAC) clones on a radiation hybrid (RH) panel, using sequence-tagged sites (STSs) and PCR. The protocol consists of two rounds of two-dimensional screening from a limited number of BACs to correspond each to an STS. In the first round, STSs are assembled in the RH bins and ordered according to PCR signals derived from 384-well microtiter plates (MTPs) in which BAC clones have been arrayed. In the second round, individual BAC clones are isolated from the MTPs to build a contig. We applied this method to a 35-Mb region spanning human chromosome 1p35-p36 and assembled 1366 BACs in 11 contigs, the longest being about 20 Mb. The working draft sequences of the human genome have been integrated into the contigs to validate the accuracy.
authors.affiliation: Gene Bank, Tsukuba Institute, Institute of Physical and Chemical Research (RIKEN), 3-1-1 Koyadai, Tsukuba, 305-0074, Japan.
authors.forename: Y Z
authors.initials: YZ
authors.lastname: Chen
authors.forename: Y
authors.initials: Y
authors.lastname: Hayashi
authors.forename: J G
authors.initials: JG
authors.lastname: Wu
authors.forename: E
authors.initials: E
authors.lastname: Takaoka
authors.forename: K
authors.initials: K
authors.lastname: Maekawa
authors.forename: N
authors.initials: N
authors.lastname: Watanabe
authors.forename: J
authors.initials: J
authors.lastname: Inazawa
authors.forename: F
authors.initials: F
authors.lastname: Hosoda
authors.forename: Y
authors.initials: Y
authors.lastname: Arai
authors.forename: M
authors.initials: M
authors.lastname: Ohki
authors.forename: H
authors.initials: H
authors.lastname: Mizushima
authors.forename: A
authors.initials: A
authors.lastname: Morohashi
authors.forename: M
authors.initials: M
authors.lastname: Ohira
authors.forename: A
authors.initials: A
authors.lastname: Nakagawara
authors.forename: S Y
authors.initials: SY
authors.lastname: Liu
authors.forename: M
authors.initials: M
authors.lastname: Hoshi
authors.forename: A
authors.initials: A
authors.lastname: Horii
authors.forename: E
authors.initials: E
authors.lastname: Soeda
mesh_terms: D002874:Chromosome Mapping / Q000379:methods*; D022202:Chromosomes, Artificial, Bacterial; D002878:Chromosomes, Human, Pair 1 / Q000235:genetics*; D020451:Contig Mapping; D004247:DNA / Q000235:genetics; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D016324:Sequence Tagged Sites
chemical_list: D004247:DNA