title: Regulation of zygotic gene activation in the preimplantation mouse embryo: global activation and repression of gene expression.
abstract: Superimposed on the activation of the embryonic genome in the preimplantation mouse embryo is the formation of a transcriptionally repressive state during the two-cell stage. This repression appears mediated at the level of chromatin structure, because it is reversed by inducing histone hyperacetylation or inhibiting the second round of DNA replication. We report that of more than 200 amplicons analyzed by mRNA differential display, about 45% of them are repressed between the two-cell and four-cell stages. This repression is scored as either a decrease in amplicon expression that occurs between the two-cell and four-cell stages or on the ability of either trichostatin A (an inhibitor of histone deacetylases) or aphidicolin (an inhibitor of replicative DNA polymerases) to increase the level of amplicon expression. Results of this study also indicate that about 16% of the amplicons analyzed likely are novel genes whose sequence doesn't correspond to sequences in the current databases, whereas about 20% of the sequences expressed during this transition likely are repetitive sequences. Lastly, inducing histone hyperacetylation in the two-cell embryos inhibits cleavage to the four-cell stage. These results suggest that genome activation is global and relatively promiscuous and that a function of the transcriptionally repressive state is to dictate the appropriate profile of gene expression that is compatible with further development.
authors.affiliation: Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104-6018, USA.
authors.forename: J
authors.initials: J
authors.lastname: Ma
authors.forename: P
authors.initials: P
authors.lastname: Svoboda
authors.forename: R M
authors.initials: RM
authors.lastname: Schultz
authors.forename: P
authors.initials: P
authors.lastname: Stein
mesh_terms: D000107:Acetylation; D000818:Animals; D016590:Aphidicolin / Q000494:pharmacology; D001345:Autoradiography; D002843:Chromatin / Q000648:ultrastructure; D002970:Cleavage Stage, Ovum; D046508:Culture Techniques; D004261:DNA Replication / Q000187:drug effects; D047108:Embryonic Development; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D005786:Gene Expression Regulation; D056572:Histone Deacetylase Inhibitors; D006657:Histones / Q000378:metabolism; D006877:Hydroxamic Acids / Q000494:pharmacology; D051379:Mice; D019384:Nucleic Acid Synthesis Inhibitors; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D012333:RNA, Messenger / Q000032:analysis; D012091:Repetitive Sequences, Nucleic Acid; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D015533:Transcriptional Activation; D015053:Zygote / Q000378:metabolism*
chemical_list: D002843:Chromatin; D004791:Enzyme Inhibitors; D056572:Histone Deacetylase Inhibitors; D006657:Histones; D006877:Hydroxamic Acids; D019384:Nucleic Acid Synthesis Inhibitors; D012333:RNA, Messenger; D016590:Aphidicolin; C012589:trichostatin A