[Expression of rasp21, C-myc, C-erbB-2, and AFP in 2-FAA induced experimental hepatocarcinogenesis].
OBJECTIVE
To investigate the distribution of oncogenes and their relationships in 2-FAA induced experimental hepatocarcinogenesis.


METHODS
Rasp21, C-myc, C-erbB-2, and AFP were detected and analyzed by the SP method of immunohistochemistry.


RESULTS
Some cells expressed rasp21, C-myc, and AFP in peripheral liver lobules and alternated hepatocyte foci in the early stage of the experiment. And with the formation and progression of hyperplastic hepatocytic nodules, expressed cells increased and often accompanied each other. Expression of C-erbB-2 appeared in the middle stage of the hepatocarcinogenesis.


CONCLUSIONS
Expression of rasp21, C-myc, and AFP in the experimental hepatocarcinogenesis is an early molecule change, which may relate to the initiation of hepatocarcinogenesis and be considered to be one of the molecular bases for the morphogenesis of hepatocarcinogenesis. During the development of lesion, C-erbB-2 may have promoting effects. It is also clear that malignant transformation of hepatocyte needs the cooperation of multiple oncogenes.
Shi
G
G
Department of Pathology, Affiliated Hospital, Nantong Medical College, Nantong 226001, China.
Sun
C
C
Han
X
X
Meng
X
X
Wang
M
M
Gu
M
M
D015073:2-Acetylaminofluorene; D000818:Animals; D008099:Liver / Q000473:pathology; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced / Q000737:chemistry* / Q000473:pathology; D008297:Male; D016271:Proto-Oncogene Proteins c-myc / Q000032:analysis*; D016283:Proto-Oncogene Proteins p21(ras) / Q000032:analysis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000032:analysis*; D000509:alpha-Fetoproteins / Q000032:analysis*
D015073:2-Acetylaminofluorene; D016271:Proto-Oncogene Proteins c-myc; D016283:Proto-Oncogene Proteins p21(ras); D018719:Erb-b2 Receptor Tyrosine Kinases; D000509:alpha-Fetoproteins