Upregulation of the netrin receptor (DCC) gene during activation of b lymphocytes and modulation by interleukins.
The DCC (deleted in colon cancer) gene has a brain restricted high expression pattern. It encodes a transmembrane protein of the immunoglobulin superfamily identified as the netrin-1 receptor. It might be a member of the so called "brain-lymphoid" molecules, which control key cell surface events. To test this hypothesis we have assessed the DCC mRNA level in human normal and malignant myeloid and lymphoid cells. A high mRNA content has been observed only in mature B cells at the secreting or presecreting stage. Expression of DCC was also assessed in the anti-CD40 model of immunopoiesis. Activation of purified tonsillar B cells by anti-CD 40 antibody strongly increased the DCC mRNA level and this effect was dramatically enhanced by the association of IL-2 + IL-10, which is a potent and selective in vitro inducer of the B cell memory phenotype. In contrast no effect has been detected after activation of T cells by anti-CD3. These data suggest that the DCC encoded netrin receptor is involved in B cell immunopoiesis.
Teyssier
J R
JR
Laboratory of Molecular Genetics, University of Burgundy, Dijon, France. teyssier@u-bourgogne.fr
Rousset
F
F
Garcia
E
E
Cornillet
P
P
Laubriet
A
A
D000328:Adult; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000502:physiology*; D001921:Brain / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000235:genetics; D002460:Cell Line; D000075389:DCC Receptor; D016163:Genes, DCC; D006801:Humans; D007156:Immunologic Memory; D016753:Interleukin-10 / Q000494:pharmacology*; D007376:Interleukin-2 / Q000494:pharmacology*; D007378:Interleukins / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects / Q000502:physiology*; D016853:Muromonab-CD3 / Q000494:pharmacology; D000075386:Netrin Receptors; D014066:Palatine Tonsil / Q000276:immunology; D012333:RNA, Messenger / Q000235:genetics; D011956:Receptors, Cell Surface / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured; D025521:Tumor Suppressor Proteins; D015854:Up-Regulation / Q000187:drug effects
D015815:Cell Adhesion Molecules; D000075389:DCC Receptor; C501909:DCC protein, human; D007376:Interleukin-2; D007378:Interleukins; D016853:Muromonab-CD3; D000075386:Netrin Receptors; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D025521:Tumor Suppressor Proteins; D016753:Interleukin-10