title: The LZTFL1 gene is a part of a transcriptional map covering 250 kb within the common eliminated region 1 (C3CER1) in 3p21.3.
abstract: Deletions on 3p have been described in a large number of human tumors, suggesting the presence of a tumor suppressor gene(s). Using the elimination test, we previously defined a 1-Mb segment from human 3p21.3 (C3CER1). Genomic sequencing allowed us to construct a transcription map covering 250 kb containing five genes. We have characterized a human leucine zipper containing gene, leucine zipper transcription factor-like 1 (LZTFL1), and its mouse orthologue (Lztfl1), which was also mapped to mouse chromosome 9F. The LZTFL1 gene has two transcript isoforms displaying alternative polyadenylation. We have localized the human orthologue of the yeast SAC1 (suppressor of actin) gene as well as characterized and mapped the mouse Sac1 gene. Furthermore, the XT3 gene was characterized, encoding a member of the Na(+)/Cl(-) neurotransmitter superfamily. It has been shown that the XT3 gene had an alternatively spliced brain-specific isoform, predicted to remove 1 of 12 putative transmembrane domains. The transcription map also includes the CC chemokine receptor 9 gene (CCR9) and the LIM domain containing gene 1 (LIMD1). This work partially defines the gene content of C3CER1 that is a prerequisite for delineation of its role in tumorigenesis.
authors.affiliation: Microbiology and Tumor Biology Center (MTC), Karolinska Institute, Stockholm, S-17177, Sweden. Hajnalka.Kiss@mtc.ki.se
authors.forename: H
authors.initials: H
authors.lastname: Kiss
authors.forename: D
authors.initials: D
authors.lastname: Kedra
authors.forename: C
authors.initials: C
authors.lastname: Kiss
authors.forename: M
authors.initials: M
authors.lastname: Kost-Alimova
authors.forename: Y
authors.initials: Y
authors.lastname: Yang
authors.forename: G
authors.initials: G
authors.lastname: Klein
authors.forename: S
authors.initials: S
authors.lastname: Imreh
authors.forename: J P
authors.initials: JP
authors.lastname: Dumanski
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D002352:Carrier Proteins / Q000235:genetics; D002872:Chromosome Deletion; D002893:Chromosomes, Human, Pair 3 / Q000235:genetics*; D017931:DNA Primers; D016147:Genes, Tumor Suppressor; D006801:Humans; D016350:Leucine Zippers / Q000235:genetics*; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D051379:Mice; D008969:Molecular Sequence Data; D018377:Neurotransmitter Agents; D020161:Physical Chromosome Mapping; D011506:Proteins / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D016415:Sequence Alignment; D014157:Transcription Factors / Q000235:genetics*; D010744:Phosphoric Monoester Hydrolases
chemical_list: D002352:Carrier Proteins; D017931:DNA Primers; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D018377:Neurotransmitter Agents; D011506:Proteins; D012333:RNA, Messenger; D014157:Transcription Factors; C431701:LZTFL1 protein, human; C486612:Lztfl1 protein, mouse; C431703:SLC6A20 protein, human; C431702:SACM1L protein, human; D010744:Phosphoric Monoester Hydrolases