Noncovalent immobilization of bovine serum albumin on oxidized cellulose.
Immobilization of bovine serum albumin (BSA) on oxidized cellulose (OC) containing carboxylic groups, a biocompatible and bioresorbable polymer, was investigated in water and different buffer solutions (pH 2-7) at 5 degrees C. The maximum amounts of BSA loaded on OC in pH 2, 3, 4, 6, and 7 buffer media were 9.82, 10.52, 8.86, 9.16, 6.05, and 2.69% (w/w), respectively. In water, the corresponding value was 9.82%. The release study was performed on powder, pellet and suspension (in castor and sesame oils) dosage forms of OC-BSA immobilization products prepared in water and pH 2 buffer, in pH 7.4 phosphate buffer at 37 degrees C. The results revealed the release of BSA to be the fastest from oil suspensions, intermediate from powders, and slowest from pellets. In conclusion, the results presented suggest that OC has the potential to be used as an immobilizing matrix for BSA and other proteins in water and pH 2-4 buffer solutions.
Kumar
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Pharamaceutics Division, College of Pharmacy, The University of Iowa, Iowa City 52242, USA. vijay-kumar@uiowa.edu
Deshpande
G S
GS
D002483:Cellulose, Oxidized / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D011485:Protein Binding; D012710:Serum Albumin, Bovine / Q000378:metabolism*
D002483:Cellulose, Oxidized; D012710:Serum Albumin, Bovine