Failure to detect an association with IL1 genotypes in European Caucasians with generalised early onset periodontitis.
OBJECTIVE
In order to elucidate the genetic background to EOP it is useful to investigate associations with genetic polymorphisms of immune response genes, whose products play a r le in the inflammatory process. The aim of this study was to examine IL1A and IL1B genetic polymorphisms in unrelated European white Caucasian patients with generalised early-onset periodontitis (GEOP).


METHODS
Polymerase chain reaction (PCR) amplification of the ILIA (-889) gene and IL1B (+3953) gene (56 patients, 56 controls) was carried out and PCR products subjected to restriction fragment length polymorphism (RFLP) analysis.


RESULTS
There were no significant differences between patients and controls for any of the genotype or allele frequencies investigated (p = 1.0). Smoking status was also included as a covariate but this did not alter the results. Furthermore, expression of the composite genotype described by Kornman and coworkers was also investigated in these subjects. No significant differences were found between patients and controls whether smoking was included as a covariate or not.


CONCLUSIONS
The lack of any association between the IL1 polymorphisms and GEOP, in the population presented here, brings into doubt the usefulness of these candidate genes as markers of susceptibility to this form of periodontitis.
Hodge
P J
PJ
Periodontal and Oral Immunology Group, University of Glasgow Dental School, UK. pjh@pennyhodge.freeserve.co.uk
Riggio
M P
MP
Kinane
D F
DF
D000293:Adolescent; D000328:Adult; D010520:Aggressive Periodontitis / Q000235:genetics / Q000276:immunology*; D000483:Alleles; D015415:Biomarkers / Q000032:analysis; D016009:Chi-Square Distribution; D016001:Confidence Intervals; D004198:Disease Susceptibility; D005060:Europe; D005260:Female; D005787:Gene Frequency; D005802:Genes, MHC Class II / Q000235:genetics; D005838:Genotype; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D007375:Interleukin-1 / Q000235:genetics*; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics; D008875:Middle Aged; D009010:Monte Carlo Method; D016017:Odds Ratio; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics; D012150:Polymorphism, Restriction Fragment Length; D012907:Smoking; D013223:Statistics as Topic; D044465:White People / Q000235:genetics*
D015415:Biomarkers; D007375:Interleukin-1