title: Characterization of the manganese-containing superoxide dismutase and its gene regulation in stress response of Schizosaccharomyces pombe.
abstract: Fission yeast Schizosaccharomyces pombe contains two superoxide dismutases (SODs), one in the cytosol and the other in mitochondria. The sod2+ gene encoding putative mitochondrial superoxide dismutase containing manganese (MnSOD) has been isolated. Purification and analysis of the sod2+ gene product revealed that it contained only manganese as a cofactor, thus verified to be a genuine MnSOD. It was localized in mitochondria as expected. Its N-terminal amino acid sequence indicated that the mitochondrial targeting sequence of 21 amino acids was removed. The native form consisted of two identical subunits. The sod2+ expression was induced by external stresses, such as treatments with superoxide generators, high osmolarity, and heat. The induction by these stress treatments depended on Wis1-Spc1 MAPK signal transduction pathway being independent of transcription factors Atf1 or Pap1. The sod2 disruption rendered cells sensitive to various superoxide-generators, heat, and high osmolarity, suggesting that the mitochondrial MnSOD acts as a general defense agent against multiple stresses.
authors.affiliation: Laboratory of Molecular Microbiology, Seoul National University, Seoul, 151-742, Korea.
authors.forename: J H
authors.initials: JH
authors.lastname: Jeong
authors.forename: E S
authors.initials: ES
authors.lastname: Kwon
authors.forename: J H
authors.initials: JH
authors.lastname: Roe
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D017931:DNA Primers; D015971:Gene Expression Regulation, Enzymologic; D015966:Gene Expression Regulation, Fungal; D008969:Molecular Sequence Data; D009997:Osmotic Pressure; D000073718:Pancreatitis-Associated Proteins; D012568:Schizosaccharomyces / Q000201:enzymology / Q000235:genetics*; D013347:Subcellular Fractions / Q000502:physiology; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism*
chemical_list: D017931:DNA Primers; D000073718:Pancreatitis-Associated Proteins; C000612141:REG3A protein, human; D013482:Superoxide Dismutase