Absence of beta-catenin gene mutations in pancreatic duct lesions induced by N-nitrosobis(2-oxopropyl)amine in hamsters.
The involvement of beta-catenin gene alterations in pancreatic duct carcinogenesis induced by N-nitrosobis(2-oxopropyl)amine (BOP) in hamsters was examined by polymerase chain reaction-single strand conformation polymorphism analysis and the expression of beta-catenin protein was examined by immunohistochemistry. No mutations of the beta-catenin gene were detected in 20 pancreatic duct adenocarcinomas (PDAs). Immunohistochemical staining showed the beta-catenin protein to be ubiquitously localized in the cell membranes. beta-Catenin accumulation was not identified in the cytoplasm and/or nucleus in any of 102 hyperplasias, 35 atypical hyperplasias, and 73 PDAs, as well as normal pancreatic duct cells. These results suggest that the Wnt/beta-catenin signaling pathway may not play an important role in pancreatic duct carcinogenesis induced by BOP in hamsters.
Kubozoe
T
T
Department of Oncological Pathology, Cancer Center, Nara Medical University, Kashihara, 634-8521, Nara, Japan.
Tsujiuchi
T
T
Murata
N
N
Sasaki
Y
Y
Tsunoda
T
T
Konishi
Y
Y
Tsutsumi
M
M
D000818:Animals; D002273:Carcinogens; D021441:Carcinoma, Pancreatic Ductal / Q000139:chemically induced* / Q000235:genetics*; D002462:Cell Membrane / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D006224:Cricetinae; D003593:Cytoplasm / Q000378:metabolism; D003598:Cytoskeletal Proteins / Q000096:biosynthesis* / Q000235:genetics*; D005260:Female; D007150:Immunohistochemistry; D008647:Mesocricetus; D009154:Mutation; D009374:Neoplasms, Experimental / Q000139:chemically induced; D009602:Nitrosamines; D010183:Pancreatic Ducts / Q000378:metabolism / Q000473:pathology; D010190:Pancreatic Neoplasms / Q000139:chemically induced* / Q000235:genetics*; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D015398:Signal Transduction; D015534:Trans-Activators; D051176:beta Catenin
D002273:Carcinogens; D003598:Cytoskeletal Proteins; D009602:Nitrosamines; D015534:Trans-Activators; D051176:beta Catenin; C013622:nitrosobis(2-oxopropyl)amine