RT-PCR detection of the Na,K-ATPase beta3-isoform in human heart.
The Na,K-ATPase is a heterodimer composed of an alpha-catalytic and a beta-glycoprotein subunit. At present, three different alpha-polypeptides (alpha1, alpha2, alpha3) and two distinct beta-isoforms (beta1 and beta2) have been detected in human heart. The aim of the present study was to determine whether or not the beta3-isoform of the Na,K-ATPase can be detected in human heart. Using the highly sensitive method of RT-PCR, we here show that human heart expresses the beta3-isoform of the Na,K-ATPase. Given the differences in pharmacological properties of the nine different Na,K-ATPase isoenzymes (containing all combinations of the subunit isoforms), the study of beta3-isoform regulation in human heart may be of interest in understanding the altered response of human myocardium to digitalis therapy during heart failure.
Pierre
S
S
Laboratoire de Biochimie Fondamentale, Moléculaire et Clinique, UFR de Pharmacie, Marseille, France
Paganelli
F
F
Sennoune
S
S
Roche
R
R
Schwinger
R H
RH
Mesana
T
T
Maixent
J M
JM
D000818:Animals; D004586:Electrophoresis; D006801:Humans; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D008099:Liver / Q000201:enzymology; D009206:Myocardium / Q000201:enzymology*; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000254:Sodium-Potassium-Exchanging ATPase / Q000235:genetics* / Q000378:metabolism*
D007527:Isoenzymes; D000254:Sodium-Potassium-Exchanging ATPase