Histopathological changes in juvenile Schistosoma haematobium harboured in hamsters treated with artemether.
Histopathological changes in juvenile Schistosoma haematobium, caused by artemether administered to the infected hamsters, were studied. Hamsters were infected with S. haematobium cercariae, and after 28 days, a single dose of artemether (300 mg/kg) was administered intragastrically. After 24 h, 72 h and 7 days, groups of two hamsters were sacrificed, and livers were removed, fixed and processed routinely, and examined by light microscopy. After 24 h, 93% of the schistosomulae examined showed degeneration, which included swelling of the tegument, adherence of inflammatory cells to the damaged tegument, collapsed and damaged intestine, and infiltration of inflammatory cells, predominantly lymphocytes. After 72 h, the intensity of damage increased, including severe swelling of the tegument, loss of definition in the internal structures, collapse of intestine accompanied by release of pigment particles to the parenchymal tissues, and emergence of dead schistosomulae. Seven days after treatment, the number of dead schistosomulae increased, and most of them developed to an early- or late stage of dead worm granuloma. Meanwhile, 12% of the schistosomulae showed a normal appearance, which suggested that those schistosomulae that had survived the treatment were recovered to normal. The results demonstrated that artemether effectively acts against the juvenile stages of S. haematobium and confirms earlier results with S. japonicum and S. mansoni.
Yang
Y
Y
Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine, 200025, Shanghai, China.
Xiao
S
S
Tanner
M
M
Utzinger
J
J
Chollet
J
J
Wu
J
J
Guo
J
J
D000818:Animals; D000077549:Artemether; D037621:Artemisinins; D006224:Cricetinae; D008099:Liver / Q000187:drug effects* / Q000473:pathology; D008297:Male; D012548:Schistosoma haematobium / Q000187:drug effects*; D012553:Schistosomiasis haematobia / Q000188:drug therapy*; D012556:Schistosomicides / Q000627:therapeutic use*; D012717:Sesquiterpenes / Q000627:therapeutic use*
D037621:Artemisinins; D012556:Schistosomicides; D012717:Sesquiterpenes; D000077549:Artemether