title: Staphylococcus aureus cell extract transcription-translation assay: firefly luciferase reporter system for evaluating protein translation inhibitors.
abstract: The promoter for the Staphylococcus aureus capsule polysaccharide synthesis gene (cap1A) was cloned in front of the firefly luciferase gene for use in a cell extract S. aureus transcription-translation system. The assay is rapid, reproducible, and sensitive and has a lower background level than the radiolabeled amino acid incorporation translation assays. We present data evaluating a transcription inhibitor and a number of protein translation inhibitors in this system.
authors.affiliation: Pharmacia Corporation, Kalamazoo, Michigan 49007, USA.
authors.forename: R W
authors.initials: RW
authors.lastname: Murray
authors.forename: E P
authors.initials: EP
authors.lastname: Melchior
authors.forename: J C
authors.initials: JC
authors.lastname: Hagadorn
authors.forename: K R
authors.initials: KR
authors.lastname: Marotti
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D003001:Cloning, Molecular; D004353:Drug Evaluation, Preclinical / Q000379:methods; D017930:Genes, Reporter; D008156:Luciferases / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D010957:Plasmids; D014176:Protein Biosynthesis / Q000187:drug effects*; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics; D014158:Transcription, Genetic / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D008156:Luciferases