title: Analysis of FISH-painted chromosomes in individuals living near the Semipalatinsk nuclear test site.
abstract: The Semipalatinsk nuclear test site (STS) is located in the Republic of Kazakhstan. A total of 498 nuclear weapons tests were conducted in this area between 1949 and 1989. The radiation exposure to people who lived close to the STS resulted mostly from the above-ground explosions. Blood samples for chromosome analysis were obtained from 10 subjects who were born before the first explosion in August 1949 and lived continuously in the village of Dolon. The individual calculated effective doses were about 3 Sv. Chromosomes 2, 4 and 8 were painted by means of the FISH technique. In total, 22,240 cells were analyzed. The mean frequency of translocations in the subjects who were irradiated during childhood (2.4/1000 cells) did not differ from the control value (3.1 translocations/1000 cells). It is assumed, therefore, that the calculated physical dose is too high. A significantly increased level of complex cells was determined, however, and this was assumed to have been induced in circulating lymphocytes. The reason for this may be the incorporation of radionuclides from fallout which were not distributed homogeneously within the body, but accumulated instead in tissues that were well supplied with peripheral blood.
authors.affiliation: Institute of Radiation Hygiene of the Federal Office for Radiation Protection, Ingolstädter Landstrasse 1, D-85764 Oberschleissheim, Germany.
authors.forename: G
authors.initials: G
authors.lastname: Stephan
authors.forename: S
authors.initials: S
authors.lastname: Pressl
authors.forename: G
authors.initials: G
authors.lastname: Koshpessova
authors.forename: B I
authors.initials: BI
authors.lastname: Gusev
mesh_terms: D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D002869:Chromosome Aberrations; D020223:Chromosome Painting; D006801:Humans; D007223:Infant; D007623:Kazakhstan; D009689:Nuclear Warfare