Plasma membrane requirements for cell fusion induced by herpes simplex virus type 1 glycoproteins gB, gD, gH and gL.
Herpes simplex virus type 1 (HSV-1) glycoproteins gB, gD and gHL are capable of inducing cell fusion when expressed from plasmid vectors in the absence of any other virus components. Fusion requires the expression of all four glycoproteins on the same membrane, since they are unable to cooperate in trans to induce syncytium formation. In addition, the fusion event is dependent on the expression of a gD receptor on target cell membranes and does not require the presence of cell-surface glycosaminoglycans.
Browne
Helena
H
Division of Virology, Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, UK1.
Bruun
Birgitte
B
Division of Virology, Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, UK1.
Minson
Tony
T
Division of Virology, Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, UK1.
D000818:Animals; D016466:CHO Cells; D019556:COS Cells; D002459:Cell Fusion; D002462:Cell Membrane / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism* / Q000821:virology; D002522:Chlorocebus aethiops; D018920:Coculture Techniques; D006224:Cricetinae; D006493:Heparin / Q000494:pharmacology; D018259:Herpesvirus 1, Human / Q000378:metabolism*; D014162:Transfection; D014357:Trypsin / Q000378:metabolism; D014709:Vero Cells; D014759:Viral Envelope Proteins / Q000235:genetics / Q000378:metabolism*
D014759:Viral Envelope Proteins; C040495:glycoprotein B, Simplexvirus; C039447:glycoprotein D, Human herpesvirus 1; C044200:glycoprotein H, herpes simplex virus type 1; C077407:glycoprotein L, Human herpesvirus 1; D006493:Heparin; D014357:Trypsin