Separation of apple procyanidins into different degrees of polymerization by high-speed counter-current chromatography.
Apple procyanidins were fractionated by high-speed counter-current chromatography in a one-step operation from apple condensed tannins using a type-J multilayer coil planet centrifuge. The separation of procyanidins was performed with a two-phase solvent system composed of methyl acetate-water (1:1) by eluting the upper phase at a flow-rate of 1.0 ml/min. Each fraction was examined by time-of-flight mass spectrometry. Procyanidins were separated according to their degrees of polymerization.
Shibusaw
Y
Y
Department of Analytical Chemistry, School of Pharmacy, Tokyo University of Pharmacy and Life Science, Hachioji, Japan.
Yanagida
A
A
Isozaki
M
M
Shindo
H
H
Ito
Y
Y
D044946:Biflavonoids; D002392:Catechin / Q000737:chemistry / Q000302:isolation & purification*; D003377:Countercurrent Distribution / Q000379:methods*; D011108:Polymers / Q000737:chemistry; D044945:Proanthocyanidins; D019685:Rosales / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D044946:Biflavonoids; D011108:Polymers; D044945:Proanthocyanidins; C017674:procyanidin; D002392:Catechin