Dexamethasone inhibits invasion of murine T cells through cultured fibroblastic monolayers.
Despite the wide clinical use of glucocorticoids in the chemotherapy of leukaemia and lymphoma, there have been limited efforts at understanding the effects of these hormones on metastasis formation. The purpose of this study was to investigate the effects of glucocorticoids on the tissue-infiltrating capability of lymphoid cells. Using an in vitro invasion assay, we found that dexamethasone, a synthetic glucocorticoid analogue, inhibited the invasion of a murine T-cell hybridoma through a monolayer of fibroblast-like cells. Even low doses of dexamethasone were effective at inhibiting cellular transmigration (EC50 = 0.4 nM). A maximal decrease was observed after an overnight culture in the presence of dexamethasone. The effect persisted for at least 24 h after removal of the drug and required the binding of the hormone to its intracellular glucocorticoid receptor. Our results suggest that the decreased invasiveness of dexamethasone-treated cells is not the consequence of reduced motility or deficient production of an autocrine factor required for cell migration. This in vitro study suggests that glucocorticoids may act to reduce dissemination of lymphoma cells in vivo.
Baus
E
E
Université Libre de Bruxelles, Département de Biologie Moléculaire, Gosselies, Belgium. ebaus@dbm.ulb.ac.be
Andris
F
F
Dewit
J
J
Van der Taelen
I
I
De Baetselier
P
P
Urbain
J
J
Leo
O
O
Verschueren
H
H
D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D003907:Dexamethasone / Q000378:metabolism / Q000494:pharmacology*; D005347:Fibroblasts / Q000502:physiology; D018799:Intercellular Adhesion Molecule-1 / Q000502:physiology; D051379:Mice; D008809:Mice, Inbred C3H; D009362:Neoplasm Metastasis / Q000517:prevention & control*; D013601:T-Lymphocytes / Q000187:drug effects* / Q000502:physiology
D018799:Intercellular Adhesion Molecule-1; D003907:Dexamethasone