title: Rapid in vivo PGC-1 mRNA upregulation in brown adipose tissue of Wistar rats by a beta(3)-adrenergic agonist and lack of effect of leptin.
abstract: Peroxisome proliferator-activated receptor-gamma coactivator-1 (PGC-1) is highly expressed in brown adipose tissue (BAT) and plays an important role in adaptive thermogenesis. The aim of this study was to assess the acute effect of a beta(3)-adrenergic agonist (Trecadrine) and leptin on the expression of PGC-1 and PPARgamma2 mRNA in BAT. Trecadrine produced a marked increase (4.5-fold) in PGC-1 mRNA compared to controls (P<0.001) without changes in PPARgamma2 mRNA, whereas leptin administration did not alter either PGC-1 or PPARgamma2 expression. These results show that selective stimulation of the beta(3)-adrenoceptor rapidly upregulates the expression of PGC-1 in brown adipocytes without a concomitant increase in PPARgamma2. Moreover, our results show that PGC-1 and PPARgamma2 expression in BAT seems not to be acutely regulated by leptin.
authors.affiliation: Department of Physiology and Nutrition, University of Navarra, 31008 Pamplona, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Gómez-Ambrosi
authors.forename: G
authors.initials: G
authors.lastname: Frühbeck
authors.forename: J A
authors.initials: JA
authors.lastname: Martínez
mesh_terms: D002001:Adipose Tissue, Brown / Q000187:drug effects* / Q000378:metabolism; D000318:Adrenergic beta-Agonists / Q000494:pharmacology*; D000818:Animals; D001592:Benzyl Alcohols / Q000494:pharmacology*; D001831:Body Temperature / Q000187:drug effects; D002352:Carrier Proteins / Q000235:genetics; D005215:Fasting; D005230:Fatty Acids, Nonesterified / Q000097:blood; D007473:Ion Channels; D020738:Leptin / Q000494:pharmacology*; D008297:Male; D008565:Membrane Proteins / Q000235:genetics; D024101:Mitochondrial Proteins; D010101:Oxygen Consumption / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000235:genetics*; D000071256:Uncoupling Protein 1; D015854:Up-Regulation / Q000187:drug effects*
chemical_list: D000318:Adrenergic beta-Agonists; D001592:Benzyl Alcohols; D002352:Carrier Proteins; D005230:Fatty Acids, Nonesterified; D007473:Ion Channels; D020738:Leptin; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; D012333:RNA, Messenger; D018160:Receptors, Cytoplasmic and Nuclear; D014157:Transcription Factors; D000071256:Uncoupling Protein 1; C111264:peroxisome-proliferator-activated receptor-gamma coactivator-1; C101319:trecadrine