title: Detection of Saccharomyces cerevisiae carboxylesterase activity after native and sodium dodecyl sulfate electrophoresis by using fluorescein diacetate as substrate.
abstract: A simple method for the visualisation of wine yeast esterase (carboxylesterase EC 3.1.1.1) activity on electrophoretic gels was developed, using the fluorescent substrate fluorescein diacetate. The zymogram system allows a sensitive detection of esterase bands in only 5 min of incubation of both native and sodium dodecyl sulfate gels.
authors.affiliation: Dipartimento di Biotecnologie Agrarie, Facoltà di Agraria, Università di Padova, Italy. lante@agripolis.unipd.it
authors.forename: G
authors.initials: G
authors.lastname: Lomolino
authors.forename: A
authors.initials: A
authors.lastname: Lante
authors.forename: A
authors.initials: A
authors.lastname: Crapisi
authors.forename: P
authors.initials: P
authors.lastname: Spettoli
authors.forename: A
authors.initials: A
authors.lastname: Curioni
mesh_terms: D043182:Carboxylesterase; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005452:Fluoresceins; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D012967:Sodium Dodecyl Sulfate; D013379:Substrate Specificity
chemical_list: D005452:Fluoresceins; D012967:Sodium Dodecyl Sulfate; D002265:Carboxylic Ester Hydrolases; D043182:Carboxylesterase; C018506:diacetylfluorescein