Rapid receptor-clustering assay to detect uropathogenic and diarrheal Escherichia coli isolates bearing adhesins of the Dr family.
Infections caused by Escherichia coli isolates expressing adhesins of the Dr family are associated with diarrhea and urinary tract infections, and these E. coli strains recognize the complement regulatory protein decay-accelerating factor (DAF) as their receptor. Clustering of the DAF receptor at the sites of bacterial adherence to epithelial cells is proposed as an alternative to PCR assay for rapid detection of Dr-positive E. coli.
Goluszko
P
P
Department of Obstetrics & Gynecology, The University of Texas Medical Branch, Galveston, TX 77555-1062, USA. pgoluszk@utmb.edu
Selvarangan
R
R
Nowicki
B J
BJ
Nowicki
S
S
Hart
A
A
Pawelczyk
E
E
Nguyen
K
K
D018830:Adhesins, Escherichia coli / Q000145:classification / Q000378:metabolism*; D018958:CD55 Antigens / Q000378:metabolism*; D003967:Diarrhea / Q000382:microbiology; D004926:Escherichia coli / Q000302:isolation & purification* / Q000378:metabolism; D004927:Escherichia coli Infections / Q000382:microbiology*; D005260:Female; D006367:HeLa Cells; D006801:Humans; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000382:microbiology; D011704:Pyelonephritis / Q000382:microbiology; D011956:Receptors, Cell Surface / Q000378:metabolism*; D013997:Time Factors
D018830:Adhesins, Escherichia coli; D018958:CD55 Antigens; D011956:Receptors, Cell Surface