Exploiting genotypic diversity of 2,4-diacetylphloroglucinol-producing Pseudomonas spp.: characterization of superior root-colonizing P. fluorescens strain Q8r1-96.
The genotypic diversity that occurs in natural populations of antagonistic microorganisms provides an enormous resource for improving biological control of plant diseases. In this study, we determined the diversity of indigenous 2,4-diacetylphloroglucinol (DAPG)-producing Pseudomonas spp. occurring on roots of wheat grown in a soil naturally suppressive to take-all disease of wheat. Among 101 isolates, 16 different groups were identified by random amplified polymorphic DNA (RAPD) analysis. One RAPD group made up 50% of the total population of DAPG-producing Pseudomonas spp. Both short- and long-term studies indicated that this dominant genotype, exemplified by P. fluorescens Q8r1-96, is highly adapted to the wheat rhizosphere. Q8r1-96 requires a much lower dose (only 10 to 100 CFU seed(-1) or soil(-1)) to establish high rhizosphere population densities (10(7) CFU g of root(-1)) than Q2-87 and 1M1-96, two genotypically different, DAPG-producing P. fluorescens strains. Q8r1-96 maintained a rhizosphere population density of approximately 10(5) CFU g of root(-1) after eight successive growth cycles of wheat in three different, raw virgin soils, whereas populations of Q2-87 and 1M1-96 dropped relatively quickly after five cycles and were not detectable after seven cycles. In short-term studies, strains Q8r1-96, Q2-87, and 1M1-96 did not differ in their ability to suppress take-all. After eight successive growth cycles, however, Q8r1-96 still provided control of take-all to the same level as obtained in the take-all suppressive soil, whereas Q2-87 and 1M1-96 gave no control anymore. Biochemical analyses indicated that the superior rhizosphere competence of Q8r1-96 is not related to in situ DAPG production levels. We postulate that certain rhizobacterial genotypes have evolved a preference for colonization of specific crops. By exploiting diversity of antagonistic rhizobacteria that share a common trait, biological control can be improved significantly.
Raaijmakers
J M
JM
Root Disease and Biological Control Research Unit, USDA-ARS, Washington State University, Pullman, Washington 99164-6430, USA. jos.raaijmakers@fyto@dpw.wau.nl
Weller
D M
DM
D000935:Antifungal Agents / Q000378:metabolism*; D001681:Biological Assay; D014644:Genetic Variation; D005838:Genotype; D010572:Pest Control, Biological / Q000379:methods; D010696:Phloroglucinol / Q000031:analogs & derivatives / Q000378:metabolism*; D018517:Plant Roots / Q000382:microbiology*; D011551:Pseudomonas fluorescens / Q000235:genetics / Q000378:metabolism*; D012988:Soil Microbiology; D014908:Triticum / Q000382:microbiology*
D000935:Antifungal Agents; C059817:2,4-diacetylphloroglucinol; D010696:Phloroglucinol