[Isotope tracer combined with trichloroacetic acid precipitation method for determination of nerve growth factor concentration in mouse plasma].
OBJECTIVE
To establish the radioisotope (125I) tracer method combined with trichloroacetic acid (TCA) precipitation for the determination of nerve growth factor (NGF) in mouse plasma.


METHODS
Radioisotope tracer labelling method combined with TCA precipitation was used to determine NGF in mouse plasma.


RESULTS
The detection limit of the method for NGF in plasma was 2.0 ng/ml. The intra-assay cv (CV) and the inter-assay CV were all less than 10%. The assay linearity was determined over the range of 12.5-400 ng/ml in plasma and the correlation coefficient was 0.9990. The average recovery of the method for NGF was 95.21%.


CONCLUSIONS
The radioisotope tracer method combined with TCA precipitation is stable, sensitive and reliable. It is suitable for the determination of NGF concentration in mouse plasma.
Tang
G
G
PUMC Hospital, CAMS and PUMC, Beijing 100730.
Jiang
G
G
Zhang
Y
Y
Chen
L
L
Wang
S
S
D000818:Animals; D011232:Chemical Precipitation; D007457:Iodine Radioisotopes; D051379:Mice; D009414:Nerve Growth Factors / Q000097:blood*; D014238:Trichloroacetic Acid
D007457:Iodine Radioisotopes; D009414:Nerve Growth Factors; D014238:Trichloroacetic Acid