Familial thoracic aortic aneurysms and dissections: genetic heterogeneity with a major locus mapping to 5q13-14.
BACKGROUND
Aneurysms and dissections affecting the ascending aorta are associated primarily with degeneration of the aortic media, called medial necrosis. Families identified with dominant inheritance of thoracic aortic aneurysms and dissections (TAA/dissections) indicate that single gene mutations can cause medial necrosis in the absence of an associated syndrome.


RESULTS
Fifteen families were identified with multiple members with TAAs/dissections. DNA from affected members from 2 of the families was used for a genome-wide search for the location of the defective gene by use of random polymorphic markers. The data were analyzed by the affected-pedigree-member method of linkage analysis. This analysis revealed 3 chromosomal loci with multiple markers demonstrating evidence of linkage to the phenotype. Linkage analysis using further markers in these regions and DNA from 15 families confirmed linkage of some of the families to 5q13-14. Genetic heterogeneity for the condition was confirmed by a heterogeneity test. Data from 9 families with the highest conditional probability of being linked to 5q were used to calculate the pairwise and multipoint logarithm of the odds (LOD) scores, with a maximum LOD of 4.74, with no recombination being obtained for the marker D5S2029. In 6 families, the phenotype was not linked to the 5q locus.


CONCLUSIONS
A major locus for familial TAAs and dissections maps to 5q13-14, with the majority (9 of 15) of the families identified demonstrating evidence of linkage to this locus. The condition is genetically heterogeneous, with 6 families not demonstrating evidence of linkage to any loci previously associated with aneurysm formation.
Guo
D
D
Department of Internal Medicine, University of Texas-Houston Medical School, Houston, TX 77030, USA.
Hasham
S
S
Kuang
S Q
SQ
Vaughan
C J
CJ
Boerwinkle
E
E
Chen
H
H
Abuelo
D
D
Dietz
H C
HC
Basson
C T
CT
Shete
S S
SS
Milewicz
D M
DM
D000784:Aortic Dissection / Q000235:genetics* / Q000473:pathology; D017545:Aortic Aneurysm, Thoracic / Q000235:genetics* / Q000473:pathology; D011508:Chondroitin Sulfate Proteoglycans / Q000235:genetics; D002874:Chromosome Mapping; D002895:Chromosomes, Human, Pair 5 / Q000235:genetics; D016326:Extracellular Matrix Proteins; D005192:Family Health; D005260:Female; D018740:Genetic Heterogeneity; D015894:Genome, Human; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D037181:Lectins, C-Type; D008126:Lod Score; D008297:Male; D018895:Microsatellite Repeats; D010375:Pedigree; D020641:Polymorphism, Single Nucleotide; D011506:Proteins / Q000235:genetics; D011509:Proteoglycans; D017422:Sequence Analysis, DNA; D019699:Thrombospondins / Q000235:genetics; D053675:Versicans; D000099249:Proteoglycan Link Protein
D011508:Chondroitin Sulfate Proteoglycans; D016326:Extracellular Matrix Proteins; D037181:Lectins, C-Type; D011506:Proteins; D011509:Proteoglycans; D019699:Thrombospondins; D053675:Versicans; D000099249:Proteoglycan Link Protein; C508322:VCAN protein, human