Enzyme-assisted preparation of isotope-labeled 1-deoxy-d-xylulose 5-phosphate.
Recombinant 1-deoxy-D-xylulose 5-phosphate synthase of Bacillus subtilis was used for the preparation of isotope-labeled 1-deoxy-D-xylulose 5-phosphate using isotope-labeled glucose and/or isotope-labeled pyruvate as starting materials. The simple one-pot methods described afford almost every conceivable isotopomer of 1-deoxy-D-xylulose 5-phosphate carrying (13)C or (14)C from commercially available precursors with an overall yield around 50%.
Hecht
S
S
Lehrstuhl für Organische Chemie und Biochemie, Technische Universität München, Lichtenbergstr. 4, D-85747 Garching, Germany.
Kis
K
K
Eisenreich
W
W
Amslinger
S
S
Wungsintaweekul
J
J
Herz
S
S
Rohdich
F
F
Bacher
A
A
D001412:Bacillus subtilis / Q000201:enzymology; D007202:Indicators and Reagents; D007553:Isotope Labeling; D009682:Magnetic Resonance Spectroscopy; D010428:Pentosephosphates / Q000138:chemical synthesis*; D010957:Plasmids; D011994:Recombinant Proteins / Q000378:metabolism; D013237:Stereoisomerism; D014166:Transferases / Q000378:metabolism*
C109460:1-deoxylulose 5-phosphate; D007202:Indicators and Reagents; D010428:Pentosephosphates; D011994:Recombinant Proteins; D014166:Transferases; C109461:deoxyxylulose-5-phosphate synthase