A novel early estrogen-regulated gene gec1 encodes a protein related to GABARAP.
We have isolated, in guinea-pig endometrial cells, an estrogen-induced 1.8 kb RNA called gec1. Screening of a guinea-pig genomic library led to identification of gec1 gene consisting of 4 exons and 3 introns. Exon 1 contains the 5'UTR and the ATG initiation codon. A guinea-pig gec1 cDNA was obtained by 5'-RACE. The 351 bp coding sequence shares 76.8% identity with that of the human GABARAP 924 bp cDNA while UTRs of the two cDNAs differ. A gec1 probe from the 3'UTR revealed a 1.9 kb mRNA in human tissues and a human GEC1 cDNA was isolated from placenta. Its coding sequence shares 93 and 79% identity with that of guinea-pig gec1 and human GABARAP, respectively. The human and guinea-pig GEC1 proteins have 100% identity. GEC1 and GABARAP proteins have 87% identity and N terminus featuring a tubulin binding motif. Thus, estrogen-regulated gec1 is a new gene which could encode a microtubule-associated protein.
Vernier-Magnin
S
S
Institut d'Etude et de Transfert de Gènes, Bâtiment INSERM, Besançon, France.
Muller
S
S
Sallot
M
M
Radom
J
J
Musard
J F
JF
Adami
P
P
Dulieu
P
P
Rémy-Martin
J P
JP
Jouvenot
M
M
Fraichard
A
A
D020413:3' Untranslated Regions; D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D051017:Apoptosis Regulatory Proteins; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D004967:Estrogens / Q000494:pharmacology; D005091:Exons; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology*; D015698:Genomic Library; D006168:Guinea Pigs; D006801:Humans; D007438:Introns; D008869:Microtubule-Associated Proteins / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010920:Placenta; D012333:RNA, Messenger / Q000096:biosynthesis; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic / Q000187:drug effects
D020413:3' Untranslated Regions; D048868:Adaptor Proteins, Signal Transducing; D051017:Apoptosis Regulatory Proteins; D018076:DNA, Complementary; D004967:Estrogens; C116741:GABARAP protein, human; C430503:GABARAPL1 protein, human; D008869:Microtubule-Associated Proteins; D012333:RNA, Messenger