title: Crystal structure of an aldehyde reductase Y50F mutant-NADP complex and its implications for substrate binding.
abstract: Pig aldehyde reductase containing the active site mutation tyrosine(50) to phenylalanine has been crystallized in the presence of the cofactor NADP(H) to a resolution of 2.2 A. This structure clearly shows loss of the tyrosine hydroxyl group and no other significant perturbations compared with previously determined structures. The mutant binds cofactor (both oxidized and reduced) more tightly than the wild-type enzyme but shows a complete lack of binding of the aldehyde reductase inhibitor barbitone, as determined by fluorescence titrations. Numerous attempts at preparing a ternary complex with a range of small aldehyde substrates were unsuccessful. This result, in addition to the inability of the mutant protein to bind the inhibitor, provides strong evidence for the proposal that the tyrosine hydroxyl group is essential for substrate binding in addition to catalysis.
authors.affiliation: Department of Biochemistry, Queen's University, Kingston, Ontario, Canada.
authors.forename: Q
authors.initials: Q
authors.lastname: Ye
authors.forename: D
authors.initials: D
authors.lastname: Hyndman
authors.forename: N
authors.initials: N
authors.lastname: Green
authors.forename: X
authors.initials: X
authors.lastname: Li
authors.forename: B
authors.initials: B
authors.lastname: Korithoski
authors.forename: Z
authors.initials: Z
authors.lastname: Jia
authors.forename: T G
authors.initials: TG
authors.lastname: Flynn
mesh_terms: D000429:Alcohol Oxidoreductases / Q000737:chemistry* / Q000235:genetics*; D019943:Amino Acid Substitution / Q000235:genetics*; D000818:Animals; D001665:Binding Sites / Q000235:genetics / Q000502:physiology; D003067:Coenzymes / Q000737:chemistry* / Q000378:metabolism; D003460:Crystallization; D016297:Mutagenesis, Site-Directed / Q000235:genetics; D013379:Substrate Specificity / Q000502:physiology; D013552:Swine; D014443:Tyrosine / Q000737:chemistry* / Q000235:genetics
chemical_list: D003067:Coenzymes; D014443:Tyrosine; D000429:Alcohol Oxidoreductases; C019642:alcohol dehydrogenase (NADP+)