Endonuclease IV homolog from Dictyostelium discoideum: sequencing and functional expression in AP endonuclease-deficient Escherichia coli.
We sequenced a gene encoding AP endonuclease DdAPN in Dictyostelium discoideum. The sequence predicts a protein of 542 amino acids, showing high homology to Escherichia coli Endonuclease IV (Endo IV). There is 45% identity to Endo IV using the C-terminal 282 amino acids of the Dictyostelium protein. The DdAPN conserves nine residues for the metal-binding identified in Endo IV. The truncated DdAPN protein containing these sites partially complemented E. coli RPC501 (xth(-), nfo(-)).
Tsuji
A
A
Division of Bioengineering, Faculty of Engineering, Toyama University, 930-8555, Toyama, Japan.
Kodaira
K
K
Inoue
M
M
Yasukawa
H
H
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D019757:Carbon-Oxygen Lyases / Q000096:biosynthesis* / Q000235:genetics* / Q000378:metabolism*; D017124:Conserved Sequence; D004249:DNA Damage; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase; D043223:Deoxyribonuclease IV (Phage T4-Induced); D004023:Dictyostelium / Q000201:enzymology* / Q000235:genetics*; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000201:enzymology*; D029968:Escherichia coli Proteins; D006861:Hydrogen Peroxide / Q000494:pharmacology; D016685:Mitomycin / Q000494:pharmacology; D008969:Molecular Sequence Data; D019384:Nucleic Acid Synthesis Inhibitors / Q000494:pharmacology; D010957:Plasmids / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
D029968:Escherichia coli Proteins; D019384:Nucleic Acid Synthesis Inhibitors; D016685:Mitomycin; D006861:Hydrogen Peroxide; D043223:Deoxyribonuclease IV (Phage T4-Induced); C468972:endonuclease IV, E coli; D019757:Carbon-Oxygen Lyases; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase