title: Erythrocyte membrane ATP binding cassette (ABC) proteins: MRP1 and CFTR as well as CD39 (ecto-apyrase) involved in RBC ATP transport and elevated blood plasma ATP of cystic fibrosis.
abstract: In addition to the better-known roles of the erythrocyte in the transport of oxygen and carbon dioxide, the concept that the red blood cell is involved in the transport and release of ATP has been evolving (J. Luthje, Blut 59, 367, 1989; G. R. Bergfeld and T. Forrester, Cardiovasc. Res. 26, 40, 1992; M. L. Ellsworth et al., Am. J. Physiol. 269, H2155, 1995; R. S. Sprague et al., Am. J. Physiol. 275, H1726, 1998). Membrane proteins involved in the release of ATP from erythrocytes now appear to include members of the ATP binding cassette (ABC) family (C. F. Higgins, Annu. Rev. Cell Biol. 8, 67, 1992; C. F. Higgins, Cell 82, 693, 1995). In addition to defining physiologically the presence of ABC proteins in RBCs, accumulating gel electrophoretic evidence suggests that the cystic fibrosis transmembrane conductance regulator (CFTR) and the multidrug resistance-associated protein (MRP1), respectively, constitute significant proteins in the red blood cell membrane. As such, this finding makes the mature erythrocyte compartment a major mammalian repository of these important ABC proteins. Because of its relative structural simplicity and ready accessibility, the erythrocyte offers an ideal system to explore details of the physiological functions of ABC proteins. Moreover, the presence of different ABC proteins in a single membrane implies that interaction among these proteins and with other membrane proteins may be the norm and not the exception in terms of modulation of their functions.
authors.affiliation: Department of Medicine, Dartmouth Medical School, Hanover, New Hampshire 03755, USA. edward.h.abraham@darmouth.edu
authors.forename: E H
authors.initials: EH
authors.lastname: Abraham
authors.forename: K M
authors.initials: KM
authors.lastname: Sterling
authors.forename: R J
authors.initials: RJ
authors.lastname: Kim
authors.forename: A Y
authors.initials: AY
authors.lastname: Salikhova
authors.forename: H B
authors.initials: HB
authors.lastname: Huffman
authors.forename: M A
authors.initials: MA
authors.lastname: Crockett
authors.forename: N
authors.initials: N
authors.lastname: Johnston
authors.forename: H W
authors.initials: HW
authors.lastname: Parker
authors.forename: W E
authors.initials: WE
authors.lastname: Boyle
authors.forename: A
authors.initials: A
authors.lastname: Hartov
authors.forename: E
authors.initials: E
authors.lastname: Demidenko
authors.forename: J
authors.initials: J
authors.lastname: Efird
authors.forename: J
authors.initials: J
authors.lastname: Kahn
authors.forename: S A
authors.initials: SA
authors.lastname: Grubman
authors.forename: D M
authors.initials: DM
authors.lastname: Jefferson
authors.forename: S C
authors.initials: SC
authors.lastname: Robson
authors.forename: J H
authors.initials: JH
authors.lastname: Thakar
authors.forename: A
authors.initials: A
authors.lastname: Lorico
authors.forename: G
authors.initials: G
authors.lastname: Rappa
authors.forename: A C
authors.initials: AC
authors.lastname: Sartorelli
authors.forename: P
authors.initials: P
authors.lastname: Okunieff
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000494:pharmacology*; D000251:Adenosine Triphosphatases / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000097:blood / Q000493:pharmacokinetics; D000818:Animals; D015703:Antigens, CD / Q000494:pharmacology; D001081:Apyrase; D001692:Biological Transport; D003550:Cystic Fibrosis / Q000097:blood* / Q000209:etiology / Q000503:physiopathology*; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000235:genetics / Q000494:pharmacology; D004910:Erythrocyte Membrane / Q000737:chemistry* / Q000378:metabolism / Q000473:pathology; D004912:Erythrocytes / Q000378:metabolism / Q000473:pathology; D006801:Humans; D051379:Mice; D018345:Mice, Knockout; D027425:ATP-Binding Cassette, Sub-Family C Proteins
chemical_list: D018528:ATP-Binding Cassette Transporters; D000251:Adenosine Triphosphatases; D000255:Adenosine Triphosphate; D015703:Antigens, CD; D001081:Apyrase; D019005:Cystic Fibrosis Transmembrane Conductance Regulator; D027425:ATP-Binding Cassette, Sub-Family C Proteins; C505032:CFTR protein, human; C067869:CD39 antigen