title: [Triterpene saponins from Pulsatilla cernua].
abstract: OBJECTIVE
  To investigate the chemical constituents of the roots of Pulsatilla cernua (Thunb.) Bercht. et Opiz.
  
  
  METHODS
  Column chromatography (including D101 macroporous resin, silica gel and ODS) and HPLC were used to separate the chemical constituents whose structures were elucidated by FAB-MS, NMR (1D and 2D) and hydrolysis methods.
  
  
  RESULTS
  Six compounds were isolated and established as hederagenin 3-O-alpha-L-rhamnopyranosyl (1-->2)-alpha-L-arabinopyranoside (pulsatilla saponin A, 1), hederagenin 3-O-beta-D-glucopyranosyl (1-->3)-alpha-L-rhamnopyranosyl (1-->2)-alpha-L- arabinopyranoside (2), hederagenin' 3-O-alpha-L-rhamnopyranosyl(1-->2)[beta-D- glucopyranosyl(1-->4)]-alpha-L-arabinopyranoside (pulsatilla saponin D, 3), 3-O-alpha-L-rhamnopyranosyl (1-->2)-alpha-L-arabinopyranosyl hederagenin 28-O-beta-D-glucopyranosyl (1-->6)-beta-D-glucopyranosyl ester (dipsacoside B, 4), 3-O-alpha-L-rhamnopyranosyl(1-->2) [beta-D-glucopyranosyl(1-->4)]-alpha-L-arabinopyranosyl hederagenin 28-O-beta-D-glucopyranosyl ester (cernuoside C, 5) and daucosterol (6), respectively.
  
  
  CONCLUSIONS
  Compound 5 is a new compound and named as cernuoside C. Compounds 2, 4 and 6 were isolated from Pulsatilla cernua for the first time.
authors.affiliation: Department of Phytochemistry, China Pharmaceutical University, Nanjing 210009, China.
authors.forename: Q W
authors.initials: QW
authors.lastname: Zhang
authors.forename: W C
authors.initials: WC
authors.lastname: Ye
authors.forename: C T
authors.initials: CT
authors.lastname: Che
authors.forename: S X
authors.initials: SX
authors.lastname: Zhao
mesh_terms: D018517:Plant Roots / Q000737:chemistry*; D029626:Ranunculaceae / Q000737:chemistry*; D012503:Saponins / Q000737:chemistry* / Q000302:isolation & purification; D014315:Triterpenes / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D012503:Saponins; D014315:Triterpenes