title: Arrest of rat molar tooth development by lactational exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin.
abstract: The interference with tooth development by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) was studied in dioxin-resistant Han/Wistar rats. Lactating dams were given a single dose of 50 or 1000 microg TCDD/kg body wt 1 day after delivery and the pup heads were analyzed radiographically or histologically at postnatal days 9 and/or 22. Of 19 animals studied histologically, 10 lacked one or more third molars, which were at the bud stage at the start of the experiment. A higher proportion of pups exposed to the higher dose (9/13) lacked third molars than those exposed to the lower dose (1/6) (27/52 and 2/24 teeth missing, respectively). Missing upper third molars (19/38) were more frequent than were lower (10/38). The development of the third molars present was retarded. The root tips of the more advanced first and second molars were prematurely closed and root formation was arrested, but eruption was not affected. Dentinogenesis of the continuously erupting lower incisor teeth was preeruptively arrested because of pulpal cell death. All the teeth of the control rat pups developed normally. In contrast to the control pups, none of the 11 experimental pups examined radiographically (6 exposed to the higher dose and 5 to the lower) showed mineralization of their third molar cusps. The results show that the effects of TCDD on rat tooth development depend on not only the dose but also the tooth type and developmental stage. Inasmuch as early tooth development is under the control of inductive interactions between the epithelium and the mesenchyme, the interference by TCDD with tooth morphogenesis with the consequent arrest of development is likely to involve epithelial-mesenchymal signaling.
authors.affiliation: Department of Oral Pathology, Institute of Dentistry, University of Helsinki, Helsinki, Finland.
authors.forename: P L
authors.initials: PL
authors.lastname: Lukinmaa
authors.forename: C
authors.initials: C
authors.lastname: Sahlberg
authors.forename: A
authors.initials: A
authors.lastname: Leppäniemi
authors.forename: A M
authors.initials: AM
authors.lastname: Partanen
authors.forename: O
authors.initials: O
authors.lastname: Kovero
authors.forename: R
authors.initials: R
authors.lastname: Pohjanvirta
authors.forename: J
authors.initials: J
authors.lastname: Tuomisto
authors.forename: S
authors.initials: S
authors.lastname: Alaluusua
mesh_terms: D000818:Animals; D004785:Environmental Pollutants / Q000633:toxicity*; D005260:Female; D007180:Incisor / Q000000981:diagnostic imaging / Q000254:growth & development; D007774:Lactation; D008297:Male; D008963:Molar / Q000000981:diagnostic imaging / Q000254:growth & development* / Q000473:pathology; D000072317:Polychlorinated Dibenzodioxins / Q000008:administration & dosage / Q000633:toxicity*; D011859:Radiography; D051381:Rats; D017208:Rats, Wistar; D014071:Tooth Abnormalities / Q000139:chemically induced / Q000473:pathology
chemical_list: D004785:Environmental Pollutants; D000072317:Polychlorinated Dibenzodioxins