title: The use of hydrophilic lipophilic balanced (HLB) copolymer SPE cartridges for the extraction of diclofenac from small volume paediatric plasma samples.
abstract: The extraction of diclofenac from spiked aqueous and plasma samples by liquid-liquid extraction (LLE) and solid phase extraction (SPE) methods is compared. The SPE methodology utilised a hydrophilic lipophilic balanced (HLB) copolymer as the extraction phase. Using a literature HPLC method, a calibration curve for diclofenac was constructed in the range 1.0-50.0 microg/ml. Diclofenac spiked samples (aqueous and plasma) were extracted by LLE and SPE methodologies. The SPE resulted in higher extraction efficiencies (mean 94.9%) than the LLE (mean 78.9%) with %R.S.D.s similar in both methods (3.2 vs. 2.1%, respectively). The SPE method was suitable for the extraction of diclofenac from small volume paediatric plasma samples.
authors.affiliation: The School of Pharmacy, The Queen's University of Belfast, Medical Biology Centre, 97 Lisburn Road, BT9 7BL, Northern Ireland, Belfast, UK.
authors.forename: J S
authors.initials: JS
authors.lastname: Millership
authors.forename: L G
authors.initials: LG
authors.lastname: Hare
authors.forename: M
authors.initials: M
authors.lastname: Farry
authors.forename: P S
authors.initials: PS
authors.lastname: Collier
authors.forename: J C
authors.initials: JC
authors.lastname: McElnay
authors.forename: M D
authors.initials: MD
authors.lastname: Shields
authors.forename: D J
authors.initials: DJ
authors.lastname: Carson
mesh_terms: D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000097:blood*; D002138:Calibration; D002648:Child; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004008:Diclofenac / Q000097:blood*; D006801:Humans; D012015:Reference Standards
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D004008:Diclofenac