title: Overlapping CRE and E-box promoter elements can independently regulate COX-2 gene transcription in macrophages.
abstract: Macrophage cyclooxygenase-2 (COX-2) transcription is mediated through the collaboration of different promoter elements. Here, the role of an overlapping cyclic AMP responsive element (CRE)/E-box was investigated. Nuclear proteins bound both the CRE and E-box, which synergized with other promoter elements to induce COX-2 transcription. Endotoxin induced binding of nuclear proteins to the CRE and E-box and each element independently induced higher COX-2 transcription levels than the overlapping CRE/E-box. Transcription factors associated with the CRE binding complex included c-Jun and CRE binding protein and with the E-box binding complex USF-1; their overexpression significantly induced COX-2 transcription. Therefore, both CRE and E-box promoter elements regulate COX-2 transcription in macrophages.
authors.forename: J R
authors.initials: JR
authors.lastname: Mestre
authors.forename: D E
authors.initials: DE
authors.lastname: Rivadeneira
authors.forename: P J
authors.initials: PJ
authors.lastname: Mackrell
authors.forename: M
authors.initials: M
authors.lastname: Duff
authors.forename: P P
authors.initials: PP
authors.lastname: Stapleton
authors.forename: V
authors.initials: V
authors.lastname: Mack-Strong
authors.forename: S
authors.initials: S
authors.lastname: Maddali
authors.forename: G P
authors.initials: GP
authors.lastname: Smyth
authors.forename: T
authors.initials: T
authors.lastname: Tanabe
authors.forename: J M
authors.initials: JM
authors.lastname: Daly
mesh_terms: D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D000242:Cyclic AMP / Q000378:metabolism; D051546:Cyclooxygenase 2; D004731:Endotoxins / Q000378:metabolism / Q000494:pharmacology; D005786:Gene Expression Regulation; D007527:Isoenzymes / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D008957:Models, Genetic; D009154:Mutation; D010957:Plasmids / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism*; D011485:Protein Binding; D016755:Proto-Oncogene Proteins c-jun / Q000378:metabolism; D014158:Transcription, Genetic; D014162:Transfection; D015854:Up-Regulation
chemical_list: D004731:Endotoxins; D007527:Isoenzymes; D016755:Proto-Oncogene Proteins c-jun; D000242:Cyclic AMP; D051546:Cyclooxygenase 2; D011451:Prostaglandin-Endoperoxide Synthases