title: PKA accelerates rate of force development in murine skinned myocardium expressing alpha- or beta-tropomyosin.
abstract: In myocardium, protein kinase A (PKA) is known to phosphorylate troponin I (TnI) and myosin-binding protein-C (MyBP-C). Here, we used skinned myocardial preparations from nontransgenic (NTG) mouse hearts expressing 100% alpha-tropomyosin (alpha-Tm) to examine the effects of phosphorylated TnI and MyBP-C on Ca2+ sensitivity of force and the rate constant of force redevelopment (k(tr)). Experiments were also done using transgenic (TG) myocardium expressing approximately 60% beta-Tm to test the idea that the alpha-Tm isoform is required to observe the mechanical effects of PKA phosphorylation. Compared with NTG myocardium, TG myocardium exhibited greater Ca2+ sensitivity of force and developed submaximal forces at faster rates. Treatment with PKA reduced Ca2+ sensitivity of force in NTG and TG myocardium, had no effect on maximum k(tr) in either NTG or TG myocardium, and increased the rates of submaximal force development in both kinds of myocardium. These results show that PKA-mediated phosphorylation of myofibrillar proteins significantly alters the static and dynamic mechanical properties of myocardium, and these effects occur regardless of the type of Tm expressed.
authors.affiliation: Department of Physiology, University of Wisconsin Medical School, Madison, Wisconsin 53706, USA. jrpatel@physiology.wisc.edu
authors.forename: J R
authors.initials: JR
authors.lastname: Patel
authors.forename: D P
authors.initials: DP
authors.lastname: Fitzsimons
authors.forename: S H
authors.initials: SH
authors.lastname: Buck
authors.forename: M
authors.initials: M
authors.lastname: Muthuchamy
authors.forename: D F
authors.initials: DF
authors.lastname: Wieczorek
authors.forename: R L
authors.initials: RL
authors.lastname: Moss
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism / Q000494:pharmacology; D002352:Carrier Proteins / Q000378:metabolism; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism* / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D066298:In Vitro Techniques; D007537:Isometric Contraction / Q000187:drug effects / Q000502:physiology; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D009124:Muscle Proteins / Q000032:analysis / Q000378:metabolism; D009200:Myocardial Contraction / Q000187:drug effects / Q000502:physiology*; D009206:Myocardium / Q000378:metabolism*; D009210:Myofibrils / Q000187:drug effects / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D013314:Stress, Mechanical; D014335:Tropomyosin / Q000235:genetics / Q000378:metabolism*; D019210:Troponin I / Q000378:metabolism; D000099163:Myosin Binding Protein C
chemical_list: D002118:Calcium; D002352:Carrier Proteins; D017868:Cyclic AMP-Dependent Protein Kinases; D009124:Muscle Proteins; D014335:Tropomyosin; D019210:Troponin I; D000099163:Myosin Binding Protein C