Derivatization and chromatographic behavior of the o-phthaldialdehyde amino acid derivatives obtained with various SH-group-containing additives.
An overview is presented of HPLC methods currently in use to determine amino acids as their o-phthaldialdyde derivatives in the presence of various SH-group-containing additives. Crucial points that proved to influence the stability of the amino acid OPA derivatives have been discussed in detail: (i) the mol ratios of the OPA-SH-group-containing additive amino acid; (ii) the preparation and storage conditions of the OPA reagents; (iii) the optimum pH conditions for the interactions and elutions; (iv) the behavior of the, believed to be, less stable amino acids, such as glycine, beta-alanine, gamma-aminobutyric acid, histidine, ornithine and lysine.
Molnár-Perl
I
I
Institute of Inorganic and Analytical Chemistry, L. Eötvös University, Budapest, Hungary. perlne@para.chem.elte.hu
D000596:Amino Acids / Q000502:physiology*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D013438:Sulfhydryl Compounds / Q000737:chemistry*; D009764:o-Phthalaldehyde / Q000737:chemistry*
D000596:Amino Acids; D013438:Sulfhydryl Compounds; D009764:o-Phthalaldehyde