title: RNA-catalyzed RNA polymerization: accurate and general RNA-templated primer extension.
abstract: The RNA world hypothesis regarding the early evolution of life relies on the premise that some RNA sequences can catalyze RNA replication. In support of this conjecture, we describe here an RNA molecule that catalyzes the type of polymerization needed for RNA replication. The ribozyme uses nucleoside triphosphates and the coding information of an RNA template to extend an RNA primer by the successive addition of up to 14 nucleotides-more than a complete turn of an RNA helix. Its polymerization activity is general in terms of the sequence and the length of the primer and template RNAs, provided that the 3' terminus of the primer pairs with the template. Its polymerization is also quite accurate: when primers extended by 11 nucleotides were cloned and sequenced, 1088 of 1100 sequenced nucleotides matched the template.
authors.affiliation: Whitehead Institute for Biomedical Research, and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02142, USA.
authors.forename: W K
authors.initials: WK
authors.lastname: Johnston
authors.forename: P J
authors.initials: PJ
authors.lastname: Unrau
authors.forename: M S
authors.initials: MS
authors.lastname: Lawrence
authors.forename: M E
authors.initials: ME
authors.lastname: Glasner
authors.forename: D P
authors.initials: DP
authors.lastname: Bartel
mesh_terms: D001483:Base Sequence; D017124:Conserved Sequence / Q000235:genetics; D019020:Directed Molecular Evolution; D008969:Molecular Sequence Data; D016296:Mutagenesis / Q000235:genetics; D009690:Nucleic Acid Conformation; D012313:RNA / Q000096:biosynthesis* / Q000235:genetics; D016337:RNA, Catalytic / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012324:RNA-Dependent RNA Polymerase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017423:Sequence Analysis, RNA; D013379:Substrate Specificity; D013698:Templates, Genetic
chemical_list: C034477:RNA primers; D016337:RNA, Catalytic; D012313:RNA; D012324:RNA-Dependent RNA Polymerase