Development of reverse transcriptase PCR assays for detection of active human herpesvirus 6 infection.
We developed reverse transcriptase (RT) PCR assays for the detection of mRNA from three spliced genes of human herpesvirus 6 (HHV-6), the immediate-early genes U16/U17 and U89/U90 and the late gene U60/U66. Sequence analysis determined the splicing sites of these genes. The new assays may be instrumental in investigating the association between HHV-6 and disease.
Van den Bosch
G
G
Laboratory of Experimental Hematology, University of Antwerp, Antwerp University Hospital, Wilrijkstraat 10, B-2650 Edegem, Belgium.
Locatelli
G
G
Geerts
L
L
Fagà
G
G
Ieven
M
M
Goossens
H
H
Bottiger
D
D
Oberg
B
B
Lusso
P
P
Berneman
Z N
ZN
D017781:Genes, Immediate-Early; D005814:Genes, Viral; D006566:Herpesviridae Infections / Q000175:diagnosis* / Q000821:virology; D015654:Herpesvirus 6, Human / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D017874:Immediate-Early Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D012326:RNA Splicing; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000235:genetics
D017874:Immediate-Early Proteins; D012333:RNA, Messenger; D014764:Viral Proteins