title: Colonic epithelial hPepT1 expression occurs in inflammatory bowel disease: transport of bacterial peptides influences expression of MHC class 1 molecules.
abstract: OBJECTIVE
  hPepT1 is an intestinal epithelial apical membrane transporter responsible for uptake of di/tripeptides (including bacterial derived proinflammatory n-formyl peptides). hPepT1 expression normally has a strict axial gradient-highest in the proximal small intestine with no expression in the colon.
  
  
  METHODS
  Small intestinal-like cells (Caco2-BBE), and colonic-like cells (HT29-Cl.19A), and colonic mucosa from diseased and control patients were used in the present study.
  
  
  RESULTS
  hPepT1 expression occurs aberrantly in the colon with chronic ulcerative colitis (6 patients) and Crohn's disease (4 patients), but not in normal colon (4 patients) or colon with microscopic colitis (4 patients). To model expression of hPepT1 by colonic-like cells in inflamed states, we stably transfected HT29-Cl.19A cells with a modified hPepT1 tagged on the N-terminus with green fluorescence protein. Analysis of transfected cells revealed that: GFP-hPepT1 protein, like the natural protein, is targeted to the apical plasma membrane. In addition, the tagged protein retains the capability of di/tripeptide absorption, and the expression of the tagged protein by HT29-Cl.19A cells permits absorption of N-formyl-methionyl-leucyl-phenylalanine (fMLP), as occurs in hPepT1 expressing Caco2-BBE cells. fMLP uptake by colonic cells expressing GFP-hPepT1 specifically enhances major histocompatibility complex class I surface expression.
  
  
  CONCLUSIONS
  These data collectively indicate that, in some states of chronic inflammation, hPepT1 may be anomolously expressed in the colon. Further, transport of fMLP by hPepT1 potentially stimulates expression of key accessory immune molecule, MHC-1.
authors.affiliation: Epithelial Pathobiology Unit, Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia 30322, USA. dmerlin@emory.edu
authors.forename: D
authors.initials: D
authors.lastname: Merlin
authors.forename: M
authors.initials: M
authors.lastname: Si-Tahar
authors.forename: S V
authors.initials: SV
authors.lastname: Sitaraman
authors.forename: K
authors.initials: K
authors.lastname: Eastburn
authors.forename: I
authors.initials: I
authors.lastname: Williams
authors.forename: X
authors.initials: X
authors.lastname: Liu
authors.forename: M A
authors.initials: MA
authors.lastname: Hediger
authors.forename: J L
authors.initials: JL
authors.lastname: Madara
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000378:metabolism*; D001692:Biological Transport; D018938:Caco-2 Cells; D002352:Carrier Proteins / Q000032:analysis / Q000502:physiology*; D003092:Colitis / Q000378:metabolism; D003106:Colon / Q000737:chemistry; D019073:HT29 Cells; D015395:Histocompatibility Antigens Class I / Q000032:analysis*; D006801:Humans; D015212:Inflammatory Bowel Diseases / Q000378:metabolism*; D007421:Intestine, Small / Q000737:chemistry; D008969:Molecular Sequence Data; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000378:metabolism*; D000074011:Peptide Transporter 1; D027981:Symporters
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D015395:Histocompatibility Antigens Class I; D000074011:Peptide Transporter 1; C082186:SLC15A1 protein, human; D027981:Symporters; D009240:N-Formylmethionine Leucyl-Phenylalanine