[DNA-cytometry in dysplasias of the uterine cervix].
The diagnostic group of dysplasias has been described as "a group of diagnostic impotence". DNA-aneuploidy detected by image cytometry of Feulgen stained pap smears indicates a potentially progressive lesion, representing a high-grade lesion. DNA-cytometry can identify cases of dysplasias which are likely to progress: DNA-aneuploid dysplasias are HSIL. DNA-aneuploidy is the expression of an integral HPV infection. CIN-lesions with episomal HPV infections are DNA-diploid. With the newly developed laserscanning cytometer (LSC) of ThinPrep-Preparation an automatisation of ploidy measurement is possible in combination with HPV-PCR. We recommend DNA-Image-cytometry as a routine method for classification of uterine cervical borderline lesion into regressive and progressive.
Bollmann
R
R
Institut für Pathologie, Bonn. Reinhard@Bollmann.com
D000782:Aneuploidy; D015344:DNA Probes, HPV; D004279:DNA, Viral / Q000032:analysis*; D003937:Diagnosis, Differential; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D019044:Image Cytometry / Q000379:methods*; D065006:Papanicolaou Test; D027383:Papillomaviridae / Q000302:isolation & purification*; D011003:Ploidies; D011379:Prognosis; D002583:Uterine Cervical Neoplasms / Q000145:classification / Q000175:diagnosis* / Q000473:pathology; D014626:Vaginal Smears; D002578:Uterine Cervical Dysplasia / Q000145:classification / Q000175:diagnosis* / Q000473:pathology
D015344:DNA Probes, HPV; D004279:DNA, Viral