Validation of DIAGNOdent for quantification of smooth-surface caries: an in vitro study.
The aims were 1) to validate a laser-based device, KaVo DIAGNOdent, for quantification of caries lesions on smooth surfaces, using histopathologic and microradiographic analyses as the gold standard, and 2) to test inter- and intra-observer agreements. We also investigated the influence on DIAGNOdent readings of the storage medium used for extracted teeth. Two observers measured independently the tooth surfaces of 40 extracted premolars that had been stored in thymol-saturated saline. After subsequent storage in neutral-buffered formalin for 14 days they were re-measured. The teeth were then sectioned for histopathologic and microradiographic analysis. The Spearman rank correlation coefficients between lesion depth and DIAGNOdent readings were 0.78-0.83 and 0.85 for teeth stored in thymol-saturated saline and formalin, respectively. Inter- and intra-observer agreements were 0.94 and 0.95 when the teeth were stored in thymol-saturated saline. The DIAGNOdent reading was almost 1.5 times higher for teeth stored in formalin than for those stored in thymol saline. The Spearman rank correlation coefficient between mineral loss in enamel (deltaZ) and DIAGNOdent readings ranged from 0.64 to 0.68. It was concluded that DIAGNOdent may be helpful for assessing smooth-surface caries, but the cut-off points need to be assessed under clinical conditions. The increase in fluorescence associated with storage of teeth in formalin warrants further investigation.
Shi
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Department of Cariology and Endodontology, Karolinska Institutet, Huddinge, Sweden.
Tranaeus
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Angmar-Månsson
B
B
D000704:Analysis of Variance; D001641:Bicuspid; D003731:Dental Caries / Q000175:diagnosis*; D003732:Dental Caries Activity Tests / Q000295:instrumentation; D005453:Fluorescence; D005557:Formaldehyde; D006801:Humans; D007834:Lasers; D016014:Linear Models; D008852:Microradiography; D015588:Observer Variation; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D018709:Statistics, Nonparametric; D013943:Thymol; D014021:Tissue Preservation / Q000379:methods
D005557:Formaldehyde; D013943:Thymol