Response of electrically stimulated cells of Pseudomonas oleovorans strain ATCC 29347 suspended in silicone oil.
A high intensity direct current was applied for more than 10 min onto a bacterial suspension of Pseudomonas oleovorans ATCC 29347 suspended in silicone oil. The application of a gradually increased electric field from 0 to 2500 V x cm(-1) resulted in a decrease of the optical density of the bacterial suspension and the occurrence of a peak current of several hundred microA for living cells instead of a linear increase (few microA) for killed or lyophilised cells. This procedure is not only a rapid way of investigating the living state of cell cultures but also an efficient experimental tool to study the cellular effects of a controlled electrical stress.
Anglade
J
J
Laboratoire de Microbiologie, IMEP, CNRS UPRES A 6116, Faculté des Sciences et Techniques de Saint-Jérome, Marseille, France. m8700576@educ005-u.3mrs.fr
Hirschler
A
A
Le Petit
J
J
Matheron
R
R
Scarpitta
A
A
Iacazio
G
G
D004558:Electric Stimulation / Q000295:instrumentation / Q000379:methods; D011549:Pseudomonas / Q000502:physiology*; D012827:Silicone Oils
D012827:Silicone Oils