Detection and determination of anabolic steroids in nutritional supplements.
A method is described for the determination of anabolic steroids including testosterone, 19-nor-4-androstene-3,17-dione, 4-androstene-3,17-dione and nandrolone in food supplements. Initial clean-up is done by HPLC followed by determination with GC/MS. A 'contaminated' food supplement was analysed and appeared to contain 19-nor-4-androstene-3,17-dione and 4-androstene-3,17-dione. One capsule of this nutritional supplement was ingested by five male volunteers. Urine samples were collected and analysed by GC/MS and GC/MS-MS. Neither the ratio testosterone/epitestosterone, nor the ratio androstenedione/epitestosterone increased significantly. Concentrations above 2 ng/ml for norandrosterone, the major metabolite of nandrolone, were detected until 48-144 h after ingestion of the food supplement.
De Cock
K J
KJ
Department of Pharmacology, Pharmacy and Toxicology, Doping Control Unit, Faculty of Veterinary Science, Ghent University, Salisburylaan 133, B-9820, Merelbeke, Belgium.
Delbeke
F T
FT
Van Eenoo
P
P
Desmet
N
N
Roels
K
K
De Backer
P
P
D045930:Anabolic Agents / Q000032:analysis* / Q000652:urine; D000735:Androstenedione / Q000031:analogs & derivatives / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D019587:Dietary Supplements / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D008297:Male; D009277:Nandrolone / Q000032:analysis; D013739:Testosterone / Q000032:analysis
D045930:Anabolic Agents; D013739:Testosterone; D000735:Androstenedione; D009277:Nandrolone; C002828:19-norandrostenedione