Comparative metabolism of albendazole and albendazole sulphoxide by different helminth parasites.
Albendazole (ABZ) is a broad-spectrum benzimidazole anthelmintic widely used in human and veterinary medicine. The aim of the current work was to characterise the sulphoxidative metabolism of ABZ, and the sulphoreduction of ABZ sulphoxide (ABZSO), by microsomal (Ms) and cytosolic (Cyt) fractions of three different helminth species: Fasciola hepatica, Moniezia expansa and Ascaris suum. After the incubation assays, parasite material was analysed by HPLC to characterise the metabolic product formed. Both the Ms and Cyt fractions of the three parasites studied were able to oxidise ABZ into ABZSO in a non-enantioselective fashion. Oxidation of ABZ was greater in the Ms fraction of the trematode (50%) than in both cestode (19%) and nematode (14%) parasites. Only the incubation of ABZSO with both subcellular fractions of M. expansa generated ABZ as a metabolic product. The results obtained here indicate that helminths have the capacity to biotransform benzimidazole compounds; however, this metabolic activity differs qualitatively and quantitatively among helminth species.
Solana
H D
HD
Laboratorio de Biología Celular y Molecular, Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de la Provincia de Buenos Aires 7000, Tandil, Argentina. hsolana@vet.unicen.edu.ar
Rodriguez
J A
JA
Lanusse
C E
CE
D015766:Albendazole / Q000031:analogs & derivatives* / Q000378:metabolism*; D000818:Animals; D000871:Anthelmintics / Q000378:metabolism*; D017165:Ascaris suum / Q000378:metabolism*; D002589:Cestoda / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D005210:Fasciola hepatica / Q000378:metabolism*; D006374:Helminthiasis, Animal / Q000469:parasitology; D008861:Microsomes / Q000378:metabolism; D010084:Oxidation-Reduction; D012756:Sheep; D012757:Sheep Diseases / Q000469:parasitology; D013552:Swine; D013553:Swine Diseases / Q000469:parasitology
D000871:Anthelmintics; D015766:Albendazole; C027186:albendazole sulfoxide