Evaluation of genetic damage induced by a nitroimidazole derivative in human lymphocytes: Tinidazole (TNZ).
One of the useful drugs in the treatment against infestations caused by Trichomonas vaginalis, Entamoeba histolytica and Giardia lamblia is Tinidazole (TNZ) 1-[2-(ethylsulfonyl) ethyl]-2-methyl-5-nitroimidazole) (Gilman R.H., Marquis G.S., Miranda E., Vestegui M., Martinez H., 1988. Rapid reinfection by Giardia lamblia after treatment in a hyperendemic third world community. Lancet i, 343-345). We decided to evaluate the potential genetic damage induced by TNZ using different biological biomarkers such as the mitotic index (MI), sister chromatid exchange (SCE) and cell proliferation kinetics (CPK). We observed a significant decrease (P<0.0005) in the MI as well as an increase (P<0.0005) in SCE frequency and no modifications in the replication index (RI). The results obtained suggest a potential genotoxic and cytotoxic effect of TNZ in human peripheral blood cultures in vitro.
López Nigro
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CIGETOX - Citogenética y Genética Toxicológica, Depto de Bioquímica Clínica, Fac. de Farmacia y Bioquímica (FFyB), UBA, Buenos Aires, Argentina.
Gadano
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Carballo
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MA
D000328:Adult; D000704:Analysis of Variance; D000994:Antitrichomonal Agents / Q000633:toxicity*; D002455:Cell Division / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000648:ultrastructure; D008297:Male; D008940:Mitotic Index; D009153:Mutagens / Q000633:toxicity*; D012854:Sister Chromatid Exchange / Q000187:drug effects; D014011:Tinidazole / Q000633:toxicity*
D000994:Antitrichomonal Agents; D009153:Mutagens; D014011:Tinidazole