The phosphocholine motif in membranes of Mycoplasma fermentans strains.
Mycoplasma fermentans strains differ in the profile of choline-containing phosphoglycolipids (PGL) present in their cell membrane. MfGL-II [Zähringer et al. (1997) J. Biol. Chem. 272, 26262-26270] was found to be the major PGL in most strains tested. However, in the pulmonary isolates, M52 and M39 the major choline-containing PGLs were MfGL-I [Matsuda et al. (1994) J. Biol. Chem. 269, 33123-33129] and MfEL, a unique choline-containing ether lipid recently identified by us [Wagner et al. (2000) Eur. J. Biochem. 267, 6276-6286]. MfGL-I, MfGL-II and MfEL were metabolically labeled by growing the cells with radioactive choline but only MfGL-I and MfGL-II [corrected] reacted with antiphosphocholine antibodies. All tested strains fused with Molt-3 cells at almost the same rate and to about the same extent and in all the strains membrane proteins that reacted with anti-phosphocholine antibodies were detected, indicating that some membrane proteins are decorated with phosphocholine moieties.
Ben-Menachem
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G
Department of Membrane and Ultrastructure Research, The Hebrew University-Hadassah Medical School, Jerusalem, Israel.
Zähringer
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U
Rottem
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S
D015153:Blotting, Western; D002460:Cell Line; D002462:Cell Membrane / Q000737:chemistry*; D006017:Glycolipids / Q000737:chemistry; D006801:Humans; D008214:Lymphocytes; D009175:Mycoplasma Infections / Q000382:microbiology; D016858:Mycoplasma fermentans / Q000737:chemistry* / Q000254:growth & development; D010767:Phosphorylcholine / Q000032:analysis*; D012141:Respiratory Tract Infections / Q000382:microbiology
D006017:Glycolipids; D010767:Phosphorylcholine