Crystallization and preliminary X-ray analysis of the complex between human CTLA-4 and B7-2.
CTLA-4 is a dimeric T-cell surface receptor responsible for transducing signals that down-regulate activated T cells upon binding B7 ligands. The disulfide-linked homodimer of the extracellular segment of human CTLA-4 and the receptor-binding domain of human B7-2 were purified and cocrystallized. Diffraction from these crystals is consistent with the monoclinic space group P2(1) (unit-cell parameters a = 47.85, b = 54.56, c = 103.09 A, beta = 91.63); native data have been collected to 3.2 A resolution.
Zhang
X
X
Department of Cell Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA.
Schwartz
J C
JC
Nathenson
S G
SG
Almo
S C
SC
D000069594:Abatacept; D015703:Antigens, CD / Q000737:chemistry* / Q000235:genetics; D000943:Antigens, Differentiation / Q000737:chemistry* / Q000235:genetics; D051940:B7-2 Antigen; D060908:CTLA-4 Antigen; D003460:Crystallization; D018360:Crystallography, X-Ray; D017353:Gene Deletion; D006801:Humans; D018796:Immunoconjugates; D008562:Membrane Glycoproteins / Q000737:chemistry* / Q000235:genetics; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry
D015703:Antigens, CD; D000943:Antigens, Differentiation; D051940:B7-2 Antigen; C497964:CD86 protein, human; D060908:CTLA-4 Antigen; C556706:CTLA4 protein, human; D018796:Immunoconjugates; D008562:Membrane Glycoproteins; D011994:Recombinant Proteins; D000069594:Abatacept