LC determination of Z-338, novel gastroprokinetic agent in dog plasma by SCX solid phase extraction.
A simple high-performance liquid chromatographic assay with using UV detection (266 nm) was developed to determine a novel gastroprokinetic agent, Z-338 in dog plasma. The extraction procedure using solid-phase extraction with a Isolute SCX column produces extremely clean eluates and a high recovery. Intra- and inter-day variabilities were lower than 5%. The limit of quantitation of the method was 2.5 ng/ml. This assay was applied to the monitoring of Z-338 concentrations in dogs after oral administration. The method also appeared rapid, simple and suitable for therapeutic Z-338 monitoring.
Furuta
S
S
Central Research Laboratories, Zeria Pharmaceutical Co., Ltd., 2512-1 Oshikiri, Kohnan-machi, Ohsato-gun, 360-0111, Saitama-ken, Japan. ken-taisya@zeria.co.jp
Miyahara
H
H
Sugimoto
T
T
Sano
H
H
D000818:Animals; D001549:Benzamides / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid; D004285:Dogs; D005765:Gastrointestinal Agents / Q000097:blood*; D008297:Male; D013844:Thiazoles / Q000097:blood* / Q000493:pharmacokinetics
D001549:Benzamides; D005765:Gastrointestinal Agents; D013844:Thiazoles; C410959:Z 338