title: Aplysia ror forms clusters on the surface of identified neuroendocrine cells.
abstract: The ror receptors are a highly conserved family of receptor tyrosine kinases genetically implicated in the establishment of cellular polarity. We have cloned a ror receptor from the marine mollusk Aplysia californica. Aplysia ror (Apror) is expressed in most developing neurons and some adult neuronal populations, including the neuroendocrine bag-cell neurons. The Apror protein is present in peripheral neuronal processes and ganglionic neuropil, implicating the kinase in the regulation of growth and/or synaptic events. In cultured bag-cell neurons, the majority of the protein is stored in intracellular organelles, whereas only a small fraction is expressed on the surface. When expressed on the cell surface, the protein is clustered on neurites, suggesting that Apror is involved in the organization of functional domains within neurons. Apror immunoreactivity partially colocalizes with the P-type calcium channel BC-alpha1A at bag-cell neuron varicosities, suggesting a role for Apror in organizing neuropeptide release sites.
authors.affiliation: Department of Pharmacology, Yale University School of Medicine, New Haven, Connecticut 06520, USA.
authors.forename: S E
authors.initials: SE
authors.lastname: McKay
authors.forename: J
authors.initials: J
authors.lastname: Hislop
authors.forename: D
authors.initials: D
authors.lastname: Scott
authors.forename: A G
authors.initials: AG
authors.lastname: Bulloch
authors.forename: L K
authors.initials: LK
authors.lastname: Kaczmarek
authors.forename: T J
authors.initials: TJ
authors.lastname: Carew
authors.forename: W S
authors.initials: WS
authors.lastname: Sossin
mesh_terms: D000367:Age Factors; D000595:Amino Acid Sequence / Q000502:physiology; D000818:Animals; D000918:Antibody Specificity; D001048:Aplysia / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism; D001483:Base Sequence / Q000502:physiology; D029742:Caenorhabditis elegans Proteins; D002451:Cell Compartmentation / Q000502:physiology; D002478:Cells, Cultured / Q000166:cytology / Q000378:metabolism; D003001:Cloning, Molecular; D017952:Ganglia, Invertebrate / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D009490:Neurosecretory Systems / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D020794:Receptor Protein-Tyrosine Kinases / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D057050:Receptor Tyrosine Kinase-like Orphan Receptors; D011956:Receptors, Cell Surface / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D029742:Caenorhabditis elegans Proteins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; C526267:CAM-1 protein, C elegans; C078716:ROR1 protein, human; D020794:Receptor Protein-Tyrosine Kinases; D057050:Receptor Tyrosine Kinase-like Orphan Receptors