Nuclear magnetic resonance micro-imaging in the investigation of plant cell metabolism.
Micro-imaging based on nuclear magnetic resonance offers the possibility to map metabolites in plant tissues non-invasively. Major metabolites such as sucrose and amino acids can be observed with high spatial resolution. Stable isotope tracers, such as (13)C-labelled metabolites can be used to measure the in vivo conversion rates in a metabolic network. This review summarizes the different nuclear magnetic resonance micro-imaging techniques that are available to obtain spatially resolved information on metabolites in plants. A short general introduction into NMR imaging techniques is provided. Particular emphasis is given to the difficulties encountered when NMR micro-imaging is applied to plant systems.
Köckenberger
W
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Magnetic Resonance Centre, School of Physics and Astronomy, University of Nottingham, Nottingham NG7 2RD, UK. Walter.Kockenberger@nottingham.ac.uk
D000596:Amino Acids / Q000378:metabolism; D002451:Cell Compartmentation; D002477:Cells / Q000378:metabolism; D008279:Magnetic Resonance Imaging; D009682:Magnetic Resonance Spectroscopy / Q000295:instrumentation / Q000379:methods*; D008846:Micromanipulation; D059828:Plant Cells; D010944:Plants / Q000378:metabolism; D013395:Sucrose / Q000378:metabolism
D000596:Amino Acids; D013395:Sucrose