5-Aminosalicylic acid protection against oxidative damage to synaptosomal membranes by alkoxyl radicals in vitro.
The antioxidant properties of 5-aminosalicylic acid in vitro were evaluated in a synaptosomal membrane system prepared from gerbil cortical synaptosomes using EPR spin labeling and spectroscopic techniques. MAL-6 (2,2,6,6-tetramethyl-4-maleimidopiperidin-1-oxyl) and 5-NS (5-nitroxide stearate) spin labels were used to assess changes in protein oxidation and membrane lipid fluidity, respectively. Synaptosomal membranes were subjected to oxidative stress by incubation with 1 mM azo-bis(isobutyronitrile) (AIBN) or 1 mM 2,2'-azobis(amidino propane) dihydrochloride (AAPH) at 37 degrees C for 30 minutes. The EPR analyses of the samples showed significant oxidation of synaptosomal proteins and a decrease in membrane fluidity. 5-Aminosalicylic acid also was evaluated by means of FRAP (the ferric reducing ability of plasma) test as a potential antioxidant. 5-Aminosalicylic acid also showed protection against the oxidation in gerbil cortical synaptosomes system caused by AIBN and AAPH. These results are consistent with the notion of antioxidant protection against free radical induced oxidative stress in synaptosomal membrane system by this agent.
Kanski
J
J
Department of Chemistry, Center of Membrane Sciences, and Sanders-Brown Center on Aging, University of Kentucky, Lexington 04506, USA.
Lauderback
C
C
Butterfield
D A
DA
D000438:Alcohols / Q000494:pharmacology*; D000578:Amidines / Q000494:pharmacology; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism; D004578:Electron Spin Resonance Spectroscopy; D005849:Gerbillinae; D007425:Intracellular Membranes / Q000187:drug effects / Q000378:metabolism; D008051:Lipid Bilayers; D008297:Male; D008560:Membrane Fluidity / Q000187:drug effects; D019804:Mesalamine / Q000494:pharmacology*; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009570:Nitriles / Q000494:pharmacology; D016877:Oxidants / Q000494:pharmacology; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D013574:Synaptosomes / Q000187:drug effects* / Q000378:metabolism*
D000438:Alcohols; D000578:Amidines; D000975:Antioxidants; D008051:Lipid Bilayers; D009419:Nerve Tissue Proteins; D009570:Nitriles; D016877:Oxidants; C059688:alkoxyl radical; D019804:Mesalamine; C046728:2,2'-azobis(2-amidinopropane); C004526:azobis(isobutyronitrile)