Modulators with convergent cellular actions elicit distinct circuit outputs.
Six neuromodulators [proctolin, Cancer borealis tachykinin-related peptide Ia, crustacean cardioactive peptide (CCAP), red pigment-concentrating hormone, TNRNFLRFamide, and pilocarpine] converge onto the same voltage-dependent inward current in stomatogastric ganglion (STG) neurons of the crab C. borealis. We show here that each of these modulators acts on a distinct subset of pyloric network neurons in the STG. To ask whether the differences in cell targets could account for their differential effects on the pyloric rhythm, we systematically compared the motor patterns produced by proctolin and CCAP. The motor patterns produced in proctolin and CCAP differed quantitatively in a number of ways. Proctolin and CCAP both act on the lateral pyloric neuron and the inferior cardiac neuron. Proctolin additionally acts on the pyloric dilator (PD) neurons, the pyloric (PY) neurons, and the ventricular dilator neuron. Using the dynamic clamp, we introduced an artificial peptide-elicited current into the PD and PY neurons, in the presence of CCAP, and converted the CCAP rhythm into a rhythm that was statistically similar to that seen in proctolin. This suggests that the differences in the network effects of these two modulators can primarily be attributed to the known differential distributions of their receptors onto distinct subsets of neurons, despite the fact that they activate the same current.
Swensen
A M
AM
Volen Center and Biology Department, Brandeis University, Waltham, Massachusetts 02454, USA. andrew_swensen@hms.harvard.edu
Marder
E
E
D000818:Animals; D003386:Brachyura; D004064:Digestive System / Q000294:innervation*; D004305:Dose-Response Relationship, Drug; D017952:Ganglia, Invertebrate / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D009415:Nerve Net / Q000187:drug effects / Q000502:physiology; D009424:Nervous System Physiological Phenomena / Q000187:drug effects*; D009474:Neurons / Q000145:classification / Q000187:drug effects* / Q000502:physiology; D009479:Neuropeptides / Q000494:pharmacology; D018377:Neurotransmitter Agents / Q000494:pharmacology*; D009842:Oligopeptides / Q000494:pharmacology; D018408:Patch-Clamp Techniques; D010507:Periodicity; D010862:Pilocarpine / Q000494:pharmacology; D011761:Pyrrolidonecarboxylic Acid / Q000031:analogs & derivatives; D015320:Tachykinins / Q000494:pharmacology
D009479:Neuropeptides; D018377:Neurotransmitter Agents; D009842:Oligopeptides; D015320:Tachykinins; C058620:crustacean cardioactive peptide; D010862:Pilocarpine; C070157:TNRNFLRFamide; C022059:red pigment-concentrating hormone; C011536:proctolin; D011761:Pyrrolidonecarboxylic Acid