[Influences of gestational hyperglycemia on the function of fetal rat pancreatic islets].
OBJECTIVE
To investigate the influences of gestational hyperglycemia on the function of fetal rat pancreatic islets.


METHODS
Twenty-eight pregnant SD rats were randomly allocated into two groups. The study group was continually infused with 30% glucose at the rate of 2 ml/min, while the controls with sterilized distilled water in the same time and at the same rate. The infusion began on day 16.5 post-coitus and lasted for 5 days until the removal of the fetuses. The functions of the beta-cells in fetal rat islets were studied by determining the content of insulin, using the tests of extracting, releasing and perfusing.


RESULTS
The data showed that the insulin content of study group (3,329.81 +/- 163.39) ng was significantly higher than that of controls (2,390.04 +/- 151.39) ng (P < 0.05); After 2 hours' incubation in Hanks solution which contained different concentration of glucose, increased insulin secretion was found in the study group (P < 0.05); When the islets were perfused, the insulin peak value of study group was higher and appeared earlier than that of controls.


CONCLUSIONS
Insulin synthesis and secretion function of beta-cells of fetal pancreas from hyperglycemia rats were enhanced and the pancreas had a hypersensitive response to glucose challenge.
Li
L
L
Shanghai First People's Hospital, Shanghai 200080.
Wang
Y
Y
Feng
Y
Y
D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000503:physiopathology; D016640:Diabetes, Gestational / Q000503:physiopathology*; D005260:Female; D005333:Fetus; D006801:Humans; D007328:Insulin / Q000096:biosynthesis* / Q000378:metabolism; D007515:Islets of Langerhans / Q000503:physiopathology*; D011247:Pregnancy; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
D007328:Insulin