title: Concentration of interleukin-1beta and neutrophil elastase activity in gingival crevicular fluid during experimental gingivitis.
abstract: OBJECTIVE
  The aim of the present study was to measure interleukin-1beta concentrations and neutrophil elastase activity in gingival crevicular fluid (GCF) during experimental gingivitis in humans.
  
  
  METHODS
  12 healthy young men participated. After prophylaxis, they performed optimal hygiene to reach plaque and gingivitis indices of or approaching zero. All oral hygiene measures were then ceased for a period of 18 days. The Quigley-Hein plaque index (PLI) and Saxer & Mühlemann papillary bleeding index (PBI) were assessed. GCF samples were taken from the mesiobuccal site of two contralateral teeth in the upper jaw by means of periopapers at baseline and on days 3, 7, 14 and 18. After measuring the gingival crevicular fluid volume (GCFV) with the Periotron 8000, the samples were analyzed in our laboratory for the detection of IL-1beta concentration by ELISA.
  
  
  RESULTS
  PLI and PBI showed a reduction prior to baseline reaching almost zero, both increasing from day 0 to day 18 (PLI=from 0.1 to 2.9, PBI=from 0 to 2.0). IL-1beta concentration increased from 229.25 ng/ml (day 0) to 526.13 ng/ml (day 18). Clinical data and IL-1beta concentrations were correlated with elastase activity (EA). No significant correlation could be demonstrated between the clinical parameters assessed and IL-1beta or EA (Spearman rank correlation coefficient). A correlation between GCFV and PBI from day 0 to day 18 could be demonstrated.
  
  
  CONCLUSIONS
  Overall, both IL-1beta and EA showed an increase from baseline throughout the whole study.
authors.affiliation: Department of Periodontology, University of Giessen, Germany. jose.gonzales@dentist.med.uni-giessen.de
authors.forename: J R
authors.initials: JR
authors.lastname: Gonzáles
authors.forename: J M
authors.initials: JM
authors.lastname: Herrmann
authors.forename: R H
authors.initials: RH
authors.lastname: Boedeker
authors.forename: P I
authors.initials: PI
authors.lastname: Francz
authors.forename: H
authors.initials: H
authors.lastname: Biesalski
authors.forename: J
authors.initials: J
authors.lastname: Meyle
mesh_terms: D000328:Adult; D003774:Dental Plaque Index; D004797:Enzyme-Linked Immunosorbent Assay; D005500:Follow-Up Studies; D005883:Gingival Crevicular Fluid / Q000737:chemistry* / Q000201:enzymology / Q000276:immunology; D005891:Gingivitis / Q000201:enzymology / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D007375:Interleukin-1 / Q000032:analysis*; D019272:Leukocyte Elastase / Q000032:analysis*; D008297:Male; D010209:Paper; D010512:Periodontal Index; D013223:Statistics as Topic; D018709:Statistics, Nonparametric
chemical_list: D007375:Interleukin-1; D019272:Leukocyte Elastase