Effects of carmustine on aminofluorene-DNA adducts formation in rat glial tumor cells.
Arylamine-DNA adducts formation in tissues has been correlated with the carcinogenic effect of heterocyclic aromatic amines. Carmustine (BCNU) is a commercially available nitrosourea derivative and chloroethyl nitrosourea compound which is applied in anti-neoplastic therapy especially in central nervous system. The effects of carmustine on Aminofluorene-DNA (AF-DNA) formation in rat glioma cells were investigated by gamma-[32p]-dATP and high performance liquid chromatography (HPLC) using 2-aminofluorene (AF) as substrate. In the presence of 8 microM carmustine, the AF-DNA adduct formation was decreased 10.6% and 15.6% in 30 microM and 60 microM of AF (p < 0.05), respectively; In the presence of 80 microM carmustine the AF-DNA adduct formation was decreased 39.6% and 40% in 30 microM and 60 microM of AF (p < 0.02), respectively. These results show that BCNU could decrease AF-DNA formation in rat glial tumor cells.
Hung
C F
CF
Department of Pediatrics, Jen-Ai Hospital, 483, Tong-Rong Road, Tali, Taichung, Taiwan.
Wong
K T
KT
Chung
J G
JG
D000818:Animals; D018906:Antineoplastic Agents, Alkylating / Q000494:pharmacology*; D002273:Carcinogens / Q000378:metabolism*; D002330:Carmustine / Q000494:pharmacology*; D018736:DNA Adducts / Q000032:analysis*; D005449:Fluorenes / Q000378:metabolism*; D005910:Glioma / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D051381:Rats; D014407:Tumor Cells, Cultured
D018906:Antineoplastic Agents, Alkylating; D002273:Carcinogens; D018736:DNA Adducts; D005449:Fluorenes; C012177:2-aminofluorene; D002330:Carmustine