[Evaluation of an immunoblotting technique for the detection of anti-SSA and anti-SSB antibodies].
Immunoblotting and ELISA methods are now employed for the detection of anti Ro/SSA and anti La/SSB antibodies sometimes more often than double immunodiffusion or counter-immunoelectrophoresis. We have developed an immunoblotting technique for the detection of anti Ro/SSA and anti-La/SSB antibodies. This assay named IB-Hela, which uses a Hela cell extract, was compared with the double immunodiffusion technique like two other commercial immunoassays (Elisa test from Sanofi-diagnostic Pasteur and Line immunoassay from Innogenetics). This evaluation shows a good agreement especially in the case of Ro/SSA antibodies detection. IB-Hela seems to be useful in the La/SSB detection with a better sensitivity than the double immunodiffusion technique. We conclude that the IB Hela is helpful for the search for anti-Ro/SSA and anti-La/SSB autoantibodies and that it can be used as an alternative method to the classical techniques of immunodiffusion and counter immunoelectrophoresis.
Yalaoui
S
S
Laboratoire d'immunologie, hôpital Charles Nicolle, boulevard 9 avril 1938, Tunis, Tunisie.
Gorgi
Y
Y
Ayed
K
K
D000974:Antibodies, Antinuclear / Q000097:blood*; D000918:Antibody Specificity; D001324:Autoantigens / Q000276:immunology*; D001327:Autoimmune Diseases / Q000097:blood / Q000276:immunology; D002457:Cell Extracts; D003240:Connective Tissue Diseases / Q000097:blood / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006367:HeLa Cells; D006801:Humans; D015151:Immunoblotting / Q000379:methods*; D005779:Immunodiffusion; D008180:Lupus Erythematosus, Systemic / Q000097:blood / Q000276:immunology; D008947:Mixed Connective Tissue Disease / Q000097:blood / Q000276:immunology; D020733:RNA, Small Cytoplasmic; D011933:Reagent Kits, Diagnostic; D012261:Ribonucleoproteins / Q000276:immunology*; D012595:Scleroderma, Systemic / Q000097:blood / Q000276:immunology; D012680:Sensitivity and Specificity; D000097585:SS-B Antigen; D000099207:SS-A Antigen
D000974:Antibodies, Antinuclear; D001324:Autoantigens; D002457:Cell Extracts; D020733:RNA, Small Cytoplasmic; D011933:Reagent Kits, Diagnostic; D012261:Ribonucleoproteins; D000097585:SS-B Antigen; D000099207:SS-A Antigen; C116006:RO60 protein, human