title: Antibodies against a truncated Staphylococcus aureus fibronectin-binding protein protect against dissemination of infection in the rat.
abstract: Staphylococcus aureus bacteraemia (SAB) originating from local infections can lead to severe secondary infections such as endocarditis. The protective effect of antibodies against secondary infections was studied in a rat model, where a local joint infection leads to bacteraemia and endocarditis on damaged aortic valves. In this study, immunizations with a truncated D2-domain of the S. aureus fibronectin-binding protein displayed on a cow-pea mosaic virus (CPMV-D) carrier induced protection against endocarditis (P < 0.05). Opsonization of S. aureus with antibodies raised against CPMV-D stimulated both neutrophil activity and macrophage phagocytosis in vitro. Furthermore, intravenous administration of these antibodies protected mice from weight loss due to SAB.
authors.affiliation: Department of Microbiology, Pathology and Immunology, Karolinska Institute, Huddinge, Sweden.
authors.forename: A
authors.initials: A
authors.lastname: Rennermalm
authors.forename: Y H
authors.initials: YH
authors.lastname: Li
authors.forename: L
authors.initials: L
authors.lastname: Bohaufs
authors.forename: C
authors.initials: C
authors.lastname: Jarstrand
authors.forename: A
authors.initials: A
authors.lastname: Brauner
authors.forename: F R
authors.initials: FR
authors.lastname: Brennan
authors.forename: J I
authors.initials: JI
authors.lastname: Flock
mesh_terms: D018829:Adhesins, Bacterial; D000595:Amino Acid Sequence; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D000918:Antibody Specificity; D016470:Bacteremia / Q000276:immunology / Q000517:prevention & control; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology*; D001428:Bacterial Vaccines / Q000235:genetics / Q000494:pharmacology; D002352:Carrier Proteins / Q000235:genetics / Q000276:immunology*; D002678:Chimera / Q000235:genetics; D017798:Comovirus / Q000235:genetics; D004697:Endocarditis, Bacterial / Q000276:immunology / Q000517:prevention & control; D005260:Female; D008969:Molecular Sequence Data; D009895:Opsonin Proteins / Q000276:immunology; D010446:Peptide Fragments / Q000235:genetics / Q000276:immunology; D051381:Rats; D017208:Rats, Wistar; D013203:Staphylococcal Infections / Q000276:immunology / Q000517:prevention & control*; D013211:Staphylococcus aureus / Q000235:genetics / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000235:genetics / Q000494:pharmacology
chemical_list: D018829:Adhesins, Bacterial; D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D002352:Carrier Proteins; D009895:Opsonin Proteins; D010446:Peptide Fragments; D014614:Vaccines, Synthetic; C078635:fibronectin-binding proteins, bacterial