[Transdermal delivery mechanisms of lecithin nanoparticles with cyclosporin A through mice skin].
OBJECTIVE
To investigate the transdermal delivery mechanisms of the flexible nano-liposomes, conventional nano-liposomes and lecithin-cholate mixed micelles through mouse skin.


METHODS
Liposomes and micelles were applied onto the mouse skin non-occlusively in vitro and in vivo, then the drug concentrations in the skin, receiver and blood were determined.


RESULTS
In vitro permeation studies showed that flexible nano-liposomes and mixed micelles transported measurable amount of cyclosporin A through the skin. Conventional liposomes precluded drugs permeate through the skin while deposited it in the skin. In vivo studies indicated that blood concentration reached peak value at 8 h after the application of flexible nano-liposomes. Both conventional nano-liposomes and mixed micelles failed to deliver measurable amount of drug into the blood.


CONCLUSIONS
Liposomes fuse with the skin. Flexible nano-liposomes penetrate through the skin under the pressure of hydration force. Mixed micelles promote transfer in state of solution.
Guo
J X
JX
Department of Pharmaceutics, China Pharmaceutical University, Nanjing 210009, China.
Ping
Q N
QN
Wu
T
T
D000279:Administration, Cutaneous; D000818:Animals; D016572:Cyclosporine / Q000008:administration & dosage / Q000493:pharmacokinetics*; D008081:Liposomes; D051379:Mice; D008823:Micelles; D010539:Permeability; D012869:Skin Absorption
D008081:Liposomes; D008823:Micelles; D016572:Cyclosporine