The Sinorhizobium meliloti nutrient-deprivation-induced tyrosine degradation gene hmgA is controlled by a novel member of the arsR family of regulatory genes.
The regulation of the nutrient-deprivation-induced Sinorhizobium meliloti homogentisate dioxygenase (hmgA) gene, involved in tyrosine degradation, was examined. hmgA expression was found to be independent of the canonical nitrogen regulation (ntr) system. To identify regulators of hmgA, secondary mutagenesis of an S. meliloti strain harboring a hmgA-luxAB reporter gene fusion (N4) was carried out using transposon Tn1721. Two independent Tn1721 insertions were found to be located in a positive regulatory gene (nitR), encoding a protein sharing amino acid sequence similarity with proteins of the ArsR family of regulators. NitR was found to be a regulator of S. meliloti hmgA expression under nitrogen deprivation conditions, suggesting the presence of a ntr-independent nitrogen deprivation regulatory system. nitR insertion mutations were shown not to affect bacterial growth, nodulation of Medicago sativa (alfalfa) plants, or symbiotic nitrogen fixation under the physiological conditions examined. Further analysis of the nitR locus revealed the presence of open reading frames encoding proteins sharing amino acid sequence similarities with an ATP-binding phosphonate transport protein (PhnN), as well as transmembrane efflux proteins.
Milcamps
A
A
MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824, USA. milcamps@pilot.msu.edu
Struffi
P
P
de Bruijn
F J
FJ
D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D003470:Culture Media; D049308:Dioxygenases; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D005798:Genes, Bacterial; D005809:Genes, Regulator; D017930:Genes, Reporter; D005816:Genetic Complementation Test; D050560:Homogentisate 1,2-Dioxygenase; D000455:Medicago sativa / Q000382:microbiology; D008969:Molecular Sequence Data; D005810:Multigene Family; D016254:Mutagenesis, Insertional; D009584:Nitrogen / Q000172:deficiency; D010105:Oxygenases / Q000235:genetics* / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D016962:Sinorhizobium meliloti / Q000201:enzymology / Q000235:genetics*; D013559:Symbiosis; D015534:Trans-Activators / Q000235:genetics*; D014443:Tyrosine / Q000378:metabolism*
D001426:Bacterial Proteins; D003470:Culture Media; C101823:NitR protein, Rhodococcus rhodochrous; D015534:Trans-Activators; D014443:Tyrosine; D010105:Oxygenases; D049308:Dioxygenases; D050560:Homogentisate 1,2-Dioxygenase; D009584:Nitrogen