A novel highly informative polyA microsatellite on the telomeric side of the INK4a/ARF locus.
The INK4a/ARF locus encodes two cell cycle-regulatory proteins, p16(INK4a)and p14(ARF). Inactivation of the p16(INK4a)(MTS1) tumor suppressor gene by mutations, promoter methylation or gene deletions is a common event in the development of many different human tumors. The present report describes a novel polyA mononucleotide repeat situated 7.2 kb on the telomeric side of the INK4a/ARF locus. This highly polymorphic microsatellite marker (heterozygote frequency: 0.78) proved to be efficient for p16 allele loss and microsatellite instability analyses in human colon cancer.
Chaubert
P
P
Institut Universitaire de Pathologie, Centre Hospitalier Universitaire Vaudois, Bugnon 25, Lausanne, CH-1011, Switzerland. pascal.chaubert@chuv.hospvd.ch
Burri
N
N
Cousin
P
P
Shaw
P
P
D000230:Adenocarcinoma / Q000235:genetics; D000483:Alleles; D003106:Colon / Q000473:pathology; D003110:Colonic Neoplasms / Q000235:genetics; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000235:genetics*; D006579:Heterozygote; D006801:Humans; D019656:Loss of Heterozygosity; D018895:Microsatellite Repeats; D009092:Mucous Membrane / Q000473:pathology; D011061:Poly A / Q000235:genetics*; D011110:Polymorphism, Genetic; D011506:Proteins / Q000235:genetics*; D016615:Telomere / Q000648:ultrastructure*; D025803:Tumor Suppressor Protein p14ARF
D019941:Cyclin-Dependent Kinase Inhibitor p16; D011506:Proteins; D025803:Tumor Suppressor Protein p14ARF; D011061:Poly A