title: Genetic variability among archival cultures of Salmonella typhimurium.
abstract: The existence in our laboratory of over 10000 Salmonella typhimurium LT2 cultures sealed in agar stab vials for 33-46 years offers an opportunity for evolutionary and mutational studies. In each of 77 vials examined, 10(3)-10(5) colony forming units per vial were recovered (less than 0.01% of the original population) even after decades of undisturbed storage. Considerable genetic variability was observed in these populations. Three genetic variables, chromosome fragment size as determined by pulsed-field gel electrophoresis, extensive mutational reversions from nutritional auxotrophy to prototrophy, and differences in protein content as assayed by sodium dodecyl sulfate polyacrylamide gel electrophoresis, were measured.
authors.affiliation: Cancer Research Center, Columbia, MO 65201, USA.
authors.forename: K
authors.initials: K
authors.lastname: Edwards
authors.forename: I
authors.initials: I
authors.lastname: Linetsky
authors.forename: C
authors.initials: C
authors.lastname: Hueser
authors.forename: A
authors.initials: A
authors.lastname: Eisenstark
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods; D005809:Genes, Regulator; D014644:Genetic Variation; D016680:Genome, Bacterial; D009154:Mutation; D012486:Salmonella typhimurium / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins