[Determination of valproic acid level in serum by high performance liquid chromatography].
A method for the determination of valproic acid level in serum with high performance liquid chromatography was established. The conditions were Zorbax ODS (250 mm x 4.6 mm, 5 microns) column, 0.8 mL/min sulfuric acid solution (pH 4.2) eluent and UV-210 nm detector. The linear range was 0.82 mumol/L to 1.8 mumol/L (r = 0.996) and the detection limit was 0.82 mumol/L. The average recovery was 98.32% +/- 2.27% and the coefficients of variation were 3.31% (n = 5) and 4.82% (n = 7) for within-day and day-to-day tests respectively. This direct method was compared with the derivatization method.
Xiao
H
H
Nanjing Brain Hospital, Nanjing Medical University, Nanjing, 210029.
Zhang
S
S
D000927:Anticonvulsants / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D014635:Valproic Acid / Q000097:blood*
D000927:Anticonvulsants; D014635:Valproic Acid