title: Oxidative stress in cystic fibrosis: dietary and metabolic factors.
abstract: OBJECTIVE
  To examine oxidative stress in CF by measuring 8-iso-PGF2alpha and antioxidant defenses, in relation to dietary intake, immune function and clinical status.
  
  
  METHODS
  We measured total plasma concentrations of 8-iso-PGF2alpha and dietary antioxidants (vitamin E, vitamin C, beta-carotene), erythrocyte antioxidant enzyme activities (glutathione peroxidase and superoxide dismutase), lung function and dietary intake in 21 CF subjects and 21 healthy age- and gender-matched controls.
  
  
  RESULTS
  Total plasma 8-iso-PGF2alpha concentration (median [quartile 1-quartile 3]) was significantly higher in CF subjects compared to controls (214 pg/mL (155-331) vs. 135 pg/mL (101-168), p = 0.001). Neutrophil, monocyte and total white cell counts were elevated in the CF group and these correlated with 8-iso-PGF2alpha concentration. Despite similar dietary intake, lower plasma antioxidant concentrations were observed in the CF group (vitamin E, p < 0.001, vitamin C, p = 0.004, beta-carotene, p = 0.001). 8-iso-PGF2alpha correlated negatively with plasma vitamin E, C and beta-carotene concentrations.
  
  
  CONCLUSIONS
  Oxidative stress is increased in CF patients, despite normal dietary antioxidant intake. The immune response appears to be a key factor causing oxidative stress. Antioxidant intervention aimed at reducing oxidative stress in CF needs to be assessed.
authors.affiliation: Discipline of Nutrition and Dietetics, University of Newcastle, Callaghan, New South Wales, Australia.
authors.forename: L G
authors.initials: LG
authors.lastname: Wood
authors.forename: D A
authors.initials: DA
authors.lastname: Fitzgerald
authors.forename: P G
authors.initials: PG
authors.lastname: Gibson
authors.forename: D M
authors.initials: DM
authors.lastname: Cooper
authors.forename: C E
authors.initials: CE
authors.lastname: Collins
authors.forename: M L
authors.initials: ML
authors.lastname: Garg
mesh_terms: D000293:Adolescent; D000975:Antioxidants / Q000032:analysis*; D016022:Case-Control Studies; D003550:Cystic Fibrosis / Q000097:blood / Q000276:immunology / Q000378:metabolism*; D015237:Dinoprost / Q000031:analogs & derivatives / Q000097:blood*; D004912:Erythrocytes / Q000201:enzymology*; D028441:F2-Isoprostanes; D005260:Female; D005979:Glutathione Peroxidase / Q000378:metabolism; D006801:Humans; D007962:Leukocytes; D008297:Male; D018384:Oxidative Stress; D012129:Respiratory Function Tests; D013482:Superoxide Dismutase / Q000378:metabolism; D014815:Vitamins / Q000008:administration & dosage / Q000097:blood*
chemical_list: D000975:Antioxidants; D028441:F2-Isoprostanes; D014815:Vitamins; C075750:8-epi-prostaglandin F2alpha; D015237:Dinoprost; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase