title: [Effect of liposome-C-erbB2 antisense oligodeoxynucleotides on human ovarian cancer cells].
abstract: OBJECTIVE
  To explore the effects of liposome-C-erbB2 antisense phosphorothioate oligo deoxynucleotides(S-ODNs) on C-erbB2 protooncogene expression and cell proliferation in human ovarian cancer cells.
  
  
  METHODS
  The effects on C-erbB2 protooncogene expression, cell proliferation and cell cycle in human ovarian cancer cells were studied by flow cytometry and 3H-thymidine incorporation.
  
  
  RESULTS
  Liposome-C-erbB2 S-ODNs could reduce C-erbB2 expression and inhibit cell proliferation in human ovarian cancer cells; the effectiveness of liposome-C-erbB2 S-ODNs on the expression of C-erbB2 was much higher than that of C-erbB2 S-ODNs, about 40 times.
  
  
  CONCLUSIONS
  The data in this study suggest that antisense therapy is an useful method of gene therapy in ovarian cancer. The effectiveness of C-erbB2 S-ODNs could be greatly increased by C-erbB2 S-ODNs encapsulated in liposomes.
authors.affiliation: Gynecology and Obstetrics Hospital, Shanghai Medical University, Shanghai 200011.
authors.forename: M
authors.initials: M
authors.lastname: Shen
authors.forename: Y
authors.initials: Y
authors.lastname: Feng
authors.forename: B
authors.initials: B
authors.lastname: Ge
mesh_terms: D018283:Cystadenocarcinoma, Papillary / Q000473:pathology*; D005260:Female; D018734:Genes, erbB-2; D006801:Humans; D008081:Liposomes; D020319:Oligodeoxyribonucleotides, Antisense / Q000494:pharmacology*; D010051:Ovarian Neoplasms / Q000473:pathology*; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000096:biosynthesis*; D014407:Tumor Cells, Cultured
chemical_list: D008081:Liposomes; D020319:Oligodeoxyribonucleotides, Antisense; D018719:Erb-b2 Receptor Tyrosine Kinases