Effect of divalent cations on antiparallel G-quartet structure of d(G4T4G4).
The thermodynamic parameters of an antiparallel G-quartet formation of d(G4T4G4) with 1 mM divalent cation (Mg(2+), Ca(2+), Mn(2+), Co(2+), and Zn(2+)) were obtained. The thermodynamic parameters showed that the divalent cation destabilizes the antiparallel G-quartet of d(G4T4G4) in the following order: Zn(2+)>Co(2+)>Mn(2+)>Mg(2+)>Ca(2+). In addition, a higher concentration of a divalent cation induced a transition from an antiparallel to a parallel G-quartet structure. These results indicate that these divalent cations are a good tool for regulating the G-quartet structures.
Miyoshi
D
D
Department of Chemistry, Faculty of Science and Engineering, Konan University, Kobe, Japan.
Nakao
A
A
Toda
T
T
Sugimoto
N
N
D002118:Calcium / Q000737:chemistry; D002412:Cations; D002851:Chromatography, High Pressure Liquid; D002942:Circular Dichroism; D003035:Cobalt / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D006147:Guanine / Q000737:chemistry*; D008274:Magnesium / Q000737:chemistry; D008345:Manganese / Q000737:chemistry; D009690:Nucleic Acid Conformation; D013696:Temperature; D013816:Thermodynamics; D015032:Zinc / Q000737:chemistry
D002412:Cations; D003035:Cobalt; D008345:Manganese; D006147:Guanine; D008274:Magnesium; D015032:Zinc; D002118:Calcium