Improved method for the rapid determination of terpenoid aldehydes in cotton.
A simple, rapid method for the extraction of cotton terpenoid aldehydes from green tissues and seed is described. Samples were treated by ultrasonification with acidified acetonitrile/water followed by centrifugation. The resulting extract was injected directly onto a C(18) HPLC column and no sample concentration or further cleanup steps were required. The level of gossypol, the most labile of the target analytes, decreased by only 2% after 12 h of storage at room temperature, thus enabling automated analysis of individual terpenoid aldehydes by HPLC. The method gives excellent reproducibility and enables large numbers of samples to be screened quickly and accurately.
Benson
C G
CG
Centre for Biostructural and Biomolecular Research, University of Western Sydney, Hawkesbury, Richmond, New South Wales, 2753, Australia. c.benson@microtech.com.au
Wyllie
S G
SG
Leach
D N
DN
Mares
C L
CL
Fitt
G P
GP
D000447:Aldehydes / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D003368:Gossypium / Q000737:chemistry*; D006072:Gossypol / Q000032:analysis; D010936:Plant Extracts / Q000032:analysis*; D015203:Reproducibility of Results; D012639:Seeds / Q000737:chemistry; D012680:Sensitivity and Specificity; D013997:Time Factors
D000447:Aldehydes; D010936:Plant Extracts; D006072:Gossypol