Crystallization and preliminary X-ray study of haem-binding protein from the bloodsucking insect Rhodnius prolixus.
Rhodnius haem-binding protein (RHBP) from the bloodsucking insect Rhodnius prolixus, a 15 kDa protein, has been crystallized using polyethylene glycol as a precipitant. X-ray diffraction data have been collected at a synchrotron source. The crystals belong to the space group P4(1(3))2(1)2, with unit-cell parameters a = b = 64.98, c = 210.68 A, and diffract beyond 2.6 A resolution.
Nagem
R A
RA
Gleb Wataghin Physics Institute, State University of Campinas, UNICAMP, Caixa Postal 6165, CEP 13083-970, Campinas, SP, Brazil.
Brandão Neto
J R
JR
Forrer
V P
VP
Sorgine
M H
MH
Paiva-Silva
G O
GO
Masuda
H
H
Meneghini
R
R
Oliveira
P L
PL
Polikarpov
I
I
D000818:Animals; D002352:Carrier Proteins / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D000081062:Heme-Binding Proteins; D006420:Hemeproteins / Q000737:chemistry*; D019476:Insect Proteins / Q000737:chemistry*; D008970:Molecular Weight; D011487:Protein Conformation; D012239:Rhodnius / Q000737:chemistry*
D002352:Carrier Proteins; D000081062:Heme-Binding Proteins; D006420:Hemeproteins; D019476:Insect Proteins