[DNA content and cell cycle phase analysis in uterine sarcomas and its clinical significance].
OBJECTIVE
To investigate the relationship among DNA content, cell cycle phase analysis with the clinical-pathological features and prognosis of uterine sarcomas.


METHODS
DNA content and cell cycle phase analysis were performed on 75 paraffin-embedded specimens of uterine sarcomas using flow cytometry.


RESULTS
Both the aneuploidy rate and DNA index (DI) in specimens from advanced stage and poorly-differentiated cases were significantly higher than those in specimens from early stage and well-differentiated cases (P < 0.05). S-phase fraction (SPF) and proliferation index (PI) were significantly higher in advanced stage than those in early stage (P < 0.05). The 5-year suvival rates for DNA aneuploid and diploid patients were 27% and 76%, respectively (P < 0.01). The 5-year suvival rates for patients with a SPF > 20% and those with SPF < or = 20% were 10% and 56% respectively (P < 0.01).


CONCLUSIONS
DI, SPF, PI and clinical stage are the most important factors affecting the prognosis.
Tan
X
X
Maternal and Children health Care Hospital of Guangdong Province, Guangzhou 510120.
Chen
G
G
D000782:Aneuploidy; D002453:Cell Cycle; D004273:DNA, Neoplasm / Q000032:analysis*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007890:Leiomyosarcoma / Q000235:genetics / Q000473:pathology; D008875:Middle Aged; D011379:Prognosis; D018203:Sarcoma, Endometrial Stromal / Q000235:genetics / Q000473:pathology; D014594:Uterine Neoplasms / Q000235:genetics* / Q000473:pathology*
D004273:DNA, Neoplasm