Unique analytical capabilities of laser scanning cytometry (LSC) that complement flow cytometry.
Flow cytometry has become an indispensable instrumentation in many disciplines of biology and medicine. There are some limitations of flow cytometry, inherent to the fact that the cells are measured in flow, which limit its usefulness in some applications. The microscope-based laser scanning cytometer (LSC) has many features similar to flow cytometry but few restrictions of the latter and therefore it is useful in many new applications. This review briefly outlines the applications that are unique to LSC, particularly related to its morphometric capabilities and the possibility of cell relocation. Potential future applications of LSC are also discussed.
Deptala
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A
Department of Hematology, Oncology and Internal Medicine, Medical Academy, Warsaw, Poland. adeptala@amwaw.edu.pl
Bedner
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E
Darzynkiewicz
Z
Z
D000818:Animals; D002467:Cell Nucleus / Q000648:ultrastructure; D003593:Cytoplasm / Q000648:ultrastructure; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D019044:Image Cytometry / Q000379:methods*; D017404:In Situ Hybridization, Fluorescence; D018613:Microscopy, Confocal / Q000379:methods*