title: Bacteriophage WO and virus-like particles in Wolbachia, an endosymbiont of arthropods.
abstract: Wolbachia are intracellular symbionts mainly found in arthropods, causing various sexual alterations on their hosts by unknown mechanisms. Here we report the results that strongly suggest that Wolbachia have virus-like particles of phage WO, which was previously identified as a prophage-like element in the Wolbachia genome. Wolbachia (strain wTai) infection in an insect was detected with the antibody against Wsp, an outer surface protein of Wolbachia, by fluorescence microscopy and immunoelectron-microscopy for the first time. Virus-like particles in Wolbachia were observed by electron-microscopy. The 0.22-microm filtrate of insect ovary contained DAPI-positive particles, and PCR analysis demonstrated that a phage WO DNA passed through the filter while Wolbachia DNA were eliminated, suggesting that the DAPI-positive particles were phage WO.
authors.affiliation: Department of Biological Sciences, Graduate School of Science, University of Tokyo, Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan. masui777@yahoo.co.jp
authors.forename: S
authors.initials: S
authors.lastname: Masui
authors.forename: H
authors.initials: H
authors.lastname: Kuroiwa
authors.forename: T
authors.initials: T
authors.lastname: Sasaki
authors.forename: M
authors.initials: M
authors.lastname: Inui
authors.forename: T
authors.initials: T
authors.lastname: Kuroiwa
authors.forename: H
authors.initials: H
authors.lastname: Ishikawa
mesh_terms: D000818:Animals; D001435:Bacteriophages / Q000235:genetics / Q000302:isolation & purification* / Q000648:ultrastructure; D001483:Base Sequence; D005260:Female; D016680:Genome, Bacterial; D006135:Gryllidae / Q000382:microbiology*; D008242:Lysogeny; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010053:Ovary / Q000382:microbiology; D013559:Symbiosis / Q000502:physiology*; D020577:Wolbachia / Q000235:genetics / Q000502:physiology* / Q000821:virology*