Structure and serological characterization of an Nepsilon-[(R)-1-carboxyethyl]-L-lysine-containing O-chain of the lipopolysaccharide of Proteus mirabilis O13.
In this paper we present the structure and describe serological properties of the O-specific polysaccharide of Proteus mirabilis O13 lipopolysaccharide, which contains a unique component: an amide of D-galacturonic acid (D-GalA) with an unusual amino acid, Nepsilon-[(R)-1-carboxyethyl]-L-lysine (alaninolysine, AlaLys). Selective chemical degradations of either GalA or AlaLys resulted in the loss of the serological reactivity of the polysaccharide with anti-O serum against P. mirabilis O13. Neither synthetic stereoisomers of AlaLys nor the isolated amide of GalA with AlaLys inhibited the reaction of the O-antiserum with the homologous lipopolysaccharide. The O-antiserum did not cross-react with the lipopolysaccharide of Providencia alcalifaciens O23 containing an amide of D-glucuronic acid with AlaLys. These data showed that both uronic acid and amino acid components of the amide play an important role in manifesting the P. mirabilis O13-specificity, but the full specific epitope also includes another O-specific polysaccharide component(s). A cross-reactivity of anti-O13 serum with some other P. mirabilis strains was observed and attributed to a common heat-stable antigen(s) different from the lipopolysaccharide.
Swierzko
A S
AS
Centre of Microbiology and Virology, Polish Academy of Sciences, Lodowa, Lódź.
Cedzyński
M
M
Ziółkowski
A
A
Senchenkova
S N
SN
Perepelov
A V
AV
Knirel
Y A
YA
Kaca
W
W
D000907:Antibodies, Bacterial; D002240:Carbohydrate Sequence; D003429:Cross Reactions; D000939:Epitopes / Q000737:chemistry; D006801:Humans; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000276:immunology*; D008969:Molecular Sequence Data; D015394:Molecular Structure; D019081:O Antigens / Q000737:chemistry / Q000276:immunology; D011513:Proteus mirabilis / Q000737:chemistry* / Q000276:immunology* / Q000472:pathogenicity; D014574:Uronic Acids / Q000737:chemistry
D000907:Antibodies, Bacterial; D000939:Epitopes; D008070:Lipopolysaccharides; D019081:O Antigens; D014574:Uronic Acids; C430212:lipopolysaccharide, Proteus