title: Sex dependent differences in physiological ageing in the immune system of lower airways in healthy non-smoking volunteers: study of lymphocyte subsets in bronchoalveolar lavage fluid and blood.
abstract: BACKGROUND
  Age related changes in the immune system have been studied frequently but a possible relation to sex has not, to our knowledge, previously been examined. The effect of age and sex on the composition of lymphocyte subsets in bronchoalveolar lavage (BAL) fluid and peripheral blood was therefore examined.
  
  
  METHODS
  Bronchoscopy with lavage was performed in 32 healthy non-atopic, non-smoking volunteers (16 women aged 26-63 years (mean 44) and 16 men aged 23-63 years (mean 39)). Cytospin preparations for differential counts of BAL fluid cells and surface antigen expression of lymphocytes from BAL fluid and blood were analysed by flow cytometry.
  
  
  RESULTS
  Most parameters in the BAL fluid changed with age in women. The percentage of CD4+ lymphocytes increased with age from a mean of 48 (SD10)% in women aged < or =40 years to 69 (11)% in women aged >43 years (p=0.001). The percentage of CD8+ lymphocytes tended to decrease with age and the CD4/CD8 ratio was 5.8 (1.2) in women aged >43 years compared with 2.1 (0.7) in those aged < or =40 years (p<0.0001). Women aged >43 years differed from men aged >43 years as well as from younger subjects of both sexes with respect to CD4+ cells and CD4/CD8 ratio, and from younger women with respect to CD8+ cells. There was no age related change in the CD4/CD8 ratio in blood. No sex related differences were seen in the blood or BAL fluid of adults below the age of 40 years.
  
  
  CONCLUSIONS
  The composition of lymphocytes with different phenotypes in the lower respiratory tract changes with age in women but not in men. This may have implications for some clinical conditions such as chronic dry cough which are observed predominantly in women.
authors.affiliation: Division of Respiratory Medicine and Allergology, Department of Medicine, Karolinska Institutet at Huddinge University Hospital, S-141 86 Huddinge, Sweden. em.mund@stockholm.mail.telia.com
authors.forename: E
authors.initials: E
authors.lastname: Mund
authors.forename: B
authors.initials: B
authors.lastname: Christensson
authors.forename: K
authors.initials: K
authors.lastname: Larsson
authors.forename: R
authors.initials: R
authors.lastname: Grönneberg
mesh_terms: D000328:Adult; D000375:Aging / Q000276:immunology*; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry* / Q000166:cytology; D016516:CD4-CD8 Ratio; D002452:Cell Count; D002469:Cell Separation; D005260:Female; D006801:Humans; D008168:Lung / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D012737:Sex Factors; D016176:T-Lymphocyte Subsets / Q000276:immunology*