PCR detection of bovine papilloma virus DNA in superficial swabs and scrapings from equine sarcoids.
The purpose of this study was to examine if bovine papilloma virus (BPV) DNA can be detected in superficial swabs or scrapings from equine sarcoids. Samples were obtained from 92 sarcoids and 20 non-sarcoidal control lesions. The polymerase chain reaction technique was used with a first primer set to check whether DNA extraction was successful, and with a second primer set specific for BPV-DNA. DNA isolation was successful in 88% of the swabs and 93% of the scrapings. All control lesions were negative for BPV-DNA.
Martens
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Department of Large Animal Surgery and Anaesthesiology, Ghent University, Salisburylaan, Belgium. ann.martens@rug.ac.be
De Moor
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Ducatelle
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R
D000818:Animals; D052688:Bovine papillomavirus 1 / Q000235:genetics / Q000302:isolation & purification*; D002417:Cattle; D017931:DNA Primers; D004279:DNA, Viral / Q000302:isolation & purification*; D006734:Horse Diseases / Q000175:diagnosis* / Q000473:pathology / Q000821:virology*; D006736:Horses; D030361:Papillomavirus Infections / Q000175:diagnosis / Q000662:veterinary*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011237:Predictive Value of Tests; D012507:Sarcoidosis / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D012680:Sensitivity and Specificity; D012878:Skin Neoplasms / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D014412:Tumor Virus Infections / Q000175:diagnosis / Q000662:veterinary*
D017931:DNA Primers; D004279:DNA, Viral