title: Heterologous acellular matrix graft for reconstruction of the rabbit urethra: histological and functional evaluation.
abstract: OBJECTIVE
  In a rabbit model we evaluated urethral replacement by a free heterologous dog acellular matrix graft and compared these results with those of a homologous graft with the exclusion of antigenicity as a major goal.
  
  
  METHODS
  In 14 male New Zealand rabbits a 0.8 to 1.1 cm. segment of urethra was resected and replaced with a tubular acellular 1.0 to 1.5 cm. (mean 1.3) urethral matrix graft placed on an 8Fr feeding tube. Seven animals received a rabbit graft, 7 received a canine graft and 3 untreated rabbits served as controls. All animals underwent urethral pressure profile determination and retrograde urethrography before 8 and 6 were sacrificed at 6 and 8 months, respectively. Grafted and normal specimens were evaluated by histological testing.
  
  
  RESULTS
  In all animals the acellular matrix graft remained in its original position. Histological examination showed complete epithelialization and progressive vessel infiltration. At 6 months more than a third of the homologous grafts had smooth muscle bundles but the heterologous grafts had only poorly disseminated smooth muscle. Picrosirius red stain demonstrated a shift in the ratio of collagen types I-to-III with an increase in type III in the processed homologous and heterologous matrices that did not change significantly postoperatively. At 8 months the urethral pressure profile detected no difference in control and matrix grafted animals, and urethrography did not readily differentiate host from implant.
  
  
  CONCLUSIONS
  In the heterologous matrix all tissue components were present after 6 months with no signs of rejection and even gradual improvement with time. However, regenerated smooth muscle did not equal that in normal rabbit urethra and it was not well oriented. Even after 8 months only a few disseminated smooth muscle cells were evident. Most alpha-actin positive cells were surrounding the vessels. Although function was normal, the alteration in the collagen ratio effected by matrix production indicated that the matrix collagen appeared not to have been replaced by host collagen. The increase in collagen type III may explain the lack of stricture in the grafted animals on normal retrourethrography.
authors.affiliation: Department of Urology, University of California School of Medicine, San Francisco, California 94143-0738, USA.
authors.forename: K D
authors.initials: KD
authors.lastname: Sievert
authors.forename: J
authors.initials: J
authors.lastname: Wefer
authors.forename: M E
authors.initials: ME
authors.lastname: Bakircioglu
authors.forename: L
authors.initials: L
authors.lastname: Nunes
authors.forename: R
authors.initials: R
authors.lastname: Dahiya
authors.forename: E A
authors.initials: EA
authors.lastname: Tanagho
mesh_terms: D000818:Animals; D003094:Collagen; D008297:Male; D023421:Models, Animal; D011817:Rabbits; D019651:Plastic Surgery Procedures; D012038:Regeneration; D014521:Urethra / Q000601:surgery*
chemical_list: D003094:Collagen