Expression of a recombinant human anti-MUC1 scFv fragment in protease-deficient Escherichia coli mutants.
The proteolytic activity of Escherichia coli periplasmic proteases can affect the expression efficiency of many heterologous proteins such as antibody fragments that are transported to the host periplasm for folding. We investigated whether four E. coli strains that were deficient in the periplasmic proteases tsp, protease III, degP and ompT, in different combinations, affect the expression levels of an anti-MUC1 scFv fragment. The ompT protease appeared to be involved in partial degradation of the scFv since degradation products were observed in all ompT unmutated strains in Western blotting, whereas such products were absent in the ompT mutated strains. The HM120 strain that contained most mutations, expressed the scFv protein efficiently but the level of functional antibody activity was low. This was probably due to an accumulation of incorrectly folded antibody molecules in the periplasm as it was characterised by low enzyme immunoassay reactions in contrast to the intense staining of the tag in Western blots. Improved understanding of the periplasmic protease involvement in the process of the antibody expression in bacteria may allow us to design host E. coli strains that are more efficient in producing functional antibodies.
Kandilogiannaki
M
M
Department of Applied Biochemistry and Immunology, Institute of Molecular Biology and Biotechnology, FORTH, Heraklion, Crete, Greece.
Koutsoudakis
G
G
Zafiropoulos
A
A
Krambovitis
E
E
D001425:Bacterial Outer Membrane Proteins; D015153:Blotting, Western; D003720:Densitometry; D010450:Endopeptidases / Q000235:genetics*; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D029968:Escherichia coli Proteins; D005821:Genetic Techniques; D006360:Heat-Shock Proteins; D006801:Humans; D007124:Immunoenzyme Techniques; D007128:Immunoglobulin Fragments / Q000737:chemistry* / Q000378:metabolism; D008666:Metalloendopeptidases / Q000235:genetics; D008954:Models, Biological; D008957:Models, Genetic; D018396:Mucin-1 / Q000737:chemistry / Q000276:immunology*; D009154:Mutation; D010446:Peptide Fragments / Q000737:chemistry / Q000276:immunology*; D010447:Peptide Hydrolases; D019897:Periplasm / Q000378:metabolism; D033903:Periplasmic Proteins; D018272:Porins / Q000235:genetics; D017510:Protein Folding; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism*; D012697:Serine Endopeptidases / Q000235:genetics
D001425:Bacterial Outer Membrane Proteins; D029968:Escherichia coli Proteins; D006360:Heat-Shock Proteins; D007128:Immunoglobulin Fragments; C116055:MUC1 tandem repeat peptide; D018396:Mucin-1; D010446:Peptide Fragments; D033903:Periplasmic Proteins; D018272:Porins; D011994:Recombinant Proteins; C499588:ompT protein, E coli; D010450:Endopeptidases; D010447:Peptide Hydrolases; C061682:DegP protease; D012697:Serine Endopeptidases; C072135:C-terminal processing peptidase; D008666:Metalloendopeptidases; C046583:pitrilysin