title: The effect of apramycin on colonization of pathogenic Escherichia coli in the intestinal tract of chicks.
abstract: The purpose of the present study was to examine the effect of apramycin sulphate on the colonization of pathogenic E. coli in the intestines of chicks. Apramycin treatment (0.5g/l in the drinking water) of 3-to 5-week-old Leghorn chicks for 24 or 48 hours resulted in a reduction, to an undetectable level, in the number of coliforms in the digestive tract for at least the first 24 h. Per os inoculation of E. coli (O2:K1) after 24 to 48 h of treatment resulted in a significant decrease in colony forming units (cfu) in the digestive tract of the treated chicks. Food deprivation from the time of inoculation did not significantly change the results. However, food and water deprivation caused bacteraemia in a number of the control chicks but not in the treated chicks. Comparison of the level of protection between Leghorn and broiler (Anak strain) chicks revealed that there was a significantly higher (P<0.05) level of bacteraemia in the broiler than in the Leghorn chicks. Chicks treated with 0.25 g/l or 0.125 g/l apramycin for 24 or 48 h before E. coli inoculation showed significantly lower cfu in the colon and caecum than untreated control chicks, but significantly higher cfu were found in the colon than in chicks treated with 0.5 g/l apramycin. Although in vitro preincubation of apramycin with ileum cells did not decrease the percentage of cells to which the bacteria adhered, the number of bacteria adhered per cell decreased significantly. Taken together, our in vitro and in vivo results show that apramycin is effective against E. coli by preventing colonization of the gut by the bacteria, which could lead to a reduction of colibacillosis in poultry.
authors.affiliation: Kimron Veterinary Institute, Bet Dagan, Israel.
authors.forename: G
authors.initials: G
authors.lastname: Leitner
authors.forename: R
authors.initials: R
authors.lastname: Waiman
authors.forename: E D
authors.initials: ED
authors.lastname: Heller
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D016470:Bacteremia / Q000209:etiology / Q000662:veterinary; D001422:Bacterial Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D002645:Chickens; D015169:Colony Count, Microbial / Q000662:veterinary; D004926:Escherichia coli / Q000187:drug effects* / Q000472:pathogenicity; D004927:Escherichia coli Infections / Q000188:drug therapy / Q000517:prevention & control / Q000662:veterinary*; D005508:Food Deprivation; D007422:Intestines / Q000382:microbiology; D009328:Nebramycin / Q000031:analogs & derivatives* / Q000494:pharmacology*; D011201:Poultry Diseases / Q000188:drug therapy / Q000382:microbiology / Q000517:prevention & control*; D014868:Water Deprivation
chemical_list: D000900:Anti-Bacterial Agents; D009328:Nebramycin; C011666:apramycin