Detergent-mediated destaining of coomassie brilliant blue-stained SDS polyacrylamide gels.
A simple and one-step detergent-mediated destaining procedure for SDS Polyacrylamide gels for proteins is described. Suspension (5%, w/v) of a commercially available household detergent, Vim Ultra, has been found to be very efficient in destaining polyacrylamide gels without interfering with the resolution of proteins. As compared to the routinely used solvent (methanol-acetic acid-water)-mediated destaining procedure, the present method is economical and user-friendly.
Pal
J K
JK
Department of Biotechnology, University of Pune, Pune 411 007, India. jkpal@unipune.ernet.in
Sarkar
A
A
Katoch
B
B
D003902:Detergents / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D007202:Indicators and Reagents / Q000737:chemistry*; D012394:Rosaniline Dyes / Q000737:chemistry*
D003902:Detergents; D007202:Indicators and Reagents; D012394:Rosaniline Dyes; C048139:Coomassie blue