[Effects of acute hypoxia on some second messengers in pulmonary and cerebral arterial endothelial and smooth muscle cells].
OBJECTIVE
The alterations of second messengers induced by acute hypoxia were studied.


METHODS
The porcine pulmonary and cerebral arterial endothelial cells (PAEC and CAEC) as well as smooth muscle cells (PASMC and CASMC) were cultured, and the hypoxic alteration of [Ca2+]i, inositol phosphates and cyclic nucleotides were measured.


RESULTS
During acute hypoxia both [Ca2+]i and cGMP levels increased and IP3, cAMP remained unchanged in PAEC, while in CAEC the intracellular Ca2+ release, the IP3, cAMP and cGMP also increased significantly. A difference in the alteration of cGMP level was observed between PASMC and CASMC.


CONCLUSIONS
The heterogeneity of endothelial cells might play a role in the different responses of pulmonary and cerebral vessels to hypoxia.
Hu
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Q
Tongji Medical University, Wuhan 430030.
Wang
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D
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D015687:Cell Hypoxia; D002478:Cells, Cultured; D002536:Cerebral Arteries / Q000166:cytology / Q000378:metabolism; D000242:Cyclic AMP / Q000378:metabolism; D006152:Cyclic GMP / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism*; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000378:metabolism*; D011651:Pulmonary Artery / Q000166:cytology / Q000378:metabolism; D015290:Second Messenger Systems; D013552:Swine
D015544:Inositol 1,4,5-Trisphosphate; D000242:Cyclic AMP; D006152:Cyclic GMP; D002118:Calcium