title: Renal mRNA response to reduced perfusion pressure conserved despite denervation in mature ovine fetuses.
abstract: We hypothesized that renal denervation in mature ovine fetuses reduces renin mRNA response to 24 h of reduced renal perfusion pressure (RPP). Seven occluder (O) (132.4 +/- 1.2 days gestation) and six control (C) (131.5 +/- 1.2 days gestation) fetuses underwent left renal denervation. Postoperatively, O fetuses experienced 24 h of reduced RPP by suprarenal aortic occlusion. Femoral arterial blood pressure (FAB) and plasma active renin (pARC) and prorenin (pPRC) concentrations were obtained hourly for 6 h and at h 23 and 24. Renin mRNA was measured by RNase protection assay. We quantitated renin containing glomeruli by immunocytochemistry. Variables were compared by ANOVA. Mean O group FAB reduction from baseline was -6.60 +/- 0.41 mmHg. pARC and pPRC increased with occlusion, renal ARC and renal PRC did not increase with occlusion. No effect in renin mRNA or number of positive glomeruli was noted with denervation in the basal state; however, significant increases were noted in response to RPP irrespective of innervation status. In conclusion, 24 h or reduced RPP in mature ovine fetus increases renal renin mRNA and the immunocytochemical expression of renin. This response is conserved despite denervation.
authors.affiliation: Department of Obstetrics and Gynecology, Perinatal Research Laboratories, Wake Forest University School of Medicine, Winston-Salem, North Carolina 27157, USA. jrosnes@wfubmc.edu
authors.forename: J S
authors.initials: JS
authors.lastname: Rosnes
authors.forename: N
authors.initials: N
authors.lastname: Valego
authors.forename: J J
authors.initials: JJ
authors.lastname: Wang
authors.forename: F M
authors.initials: FM
authors.lastname: Perez
authors.forename: J C
authors.initials: JC
authors.lastname: Rose
mesh_terms: D000818:Animals; D001794:Blood Pressure / Q000502:physiology*; D002245:Carbon Dioxide / Q000097:blood; D003714:Denervation; D004792:Enzyme Precursors / Q000097:blood / Q000378:metabolism; D005263:Femoral Artery / Q000502:physiology; D005333:Fetus / Q000378:metabolism / Q000502:physiology*; D007150:Immunohistochemistry; D007668:Kidney / Q000196:embryology* / Q000294:innervation; D007678:Kidney Glomerulus / Q000378:metabolism; D009638:Norepinephrine / Q000378:metabolism; D010100:Oxygen / Q000097:blood; D012333:RNA, Messenger / Q000378:metabolism*; D012079:Renal Circulation / Q000502:physiology*; D012083:Renin / Q000097:blood / Q000235:genetics* / Q000378:metabolism; D012756:Sheep; D013997:Time Factors
chemical_list: D004792:Enzyme Precursors; D012333:RNA, Messenger; D002245:Carbon Dioxide; D012083:Renin; D010100:Oxygen; D009638:Norepinephrine