Detection of Saccharomyces cerevisiae carboxylesterase activity after native and sodium dodecyl sulfate electrophoresis by using fluorescein diacetate as substrate.
A simple method for the visualisation of wine yeast esterase (carboxylesterase EC 3.1.1.1) activity on electrophoretic gels was developed, using the fluorescent substrate fluorescein diacetate. The zymogram system allows a sensitive detection of esterase bands in only 5 min of incubation of both native and sodium dodecyl sulfate gels.
Lomolino
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Dipartimento di Biotecnologie Agrarie, Facoltà di Agraria, Università di Padova, Italy. lante@agripolis.unipd.it
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D043182:Carboxylesterase; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005452:Fluoresceins; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D012967:Sodium Dodecyl Sulfate; D013379:Substrate Specificity
D005452:Fluoresceins; D012967:Sodium Dodecyl Sulfate; D002265:Carboxylic Ester Hydrolases; D043182:Carboxylesterase; C018506:diacetylfluorescein