Generation of an immortalized human CD4+ T cell clone inhibiting tumor growth in mice.
Tumor antigen-specific T cell clones represent a useful tool in tumor immunology; however, their long-term culture is limited. To generate an immortalized cytotoxic T cell clone against the human tumor antigen mucin, we exposed a previously generated T cell culture to Herpesvirus saimiri. We obtained an immortalized human CD4+ T cell clone, termed SITAM. Clonality of these cells was shown by analysis of the alpha/beta-T cell receptor (TCR) repertoire. Cytolytic activity was demonstrated against several mucin-expressing tumor cell lines and could not be detected against non-mucin-expressing cells. SITAM cells maintained their features stably for 2 years. Furthermore, growth of the tumor cell line Capan-2 in NOD/SCID mice was inhibited when SITAM cells were coinjected subcutaneously with tumor cells. SITAM cells provide an unlimited source of clonal T cells for analysis of tumor recognition and may be of help in TCR-targeted immunotherapy.
Pecher
G
G
AG Molecular Gene- and Immunotherapy, Humboldt-University Berlin, Charité Campus Mitte, Hessische Strasse 3-4, Berlin, 10115, Germany. gpecher@mdc-berlin.de
Harnack
U
U
Günther
M
M
Hummel
M
M
Fichtner
I
I
Schenk
J A
JA
D000818:Animals; D001483:Base Sequence; D001943:Breast Neoplasms / Q000473:pathology*; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology*; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D002999:Clone Cells; D004247:DNA; D015329:Gene Rearrangement, T-Lymphocyte; D006567:Herpesvirus 2, Saimiriine / Q000502:physiology; D006801:Humans; D051379:Mice; D016513:Mice, SCID; D014407:Tumor Cells, Cultured
D004247:DNA