title: Cloning and characterization of Aspergillus nidulans vpsA gene which is involved in vacuolar biogenesis.
abstract: In Saccharomyces cerevisiae, vacuoles play very important roles in pH and osmotic regulation, protein degradation and storage of amino acids, small ions as well as polyphosphates. In filamentous fungi, however, little is known about vacuolar functions at a molecular level. In this paper, we report the isolation of the vpsA gene from the filamentous fungus Aspergillus nidulans as a homologue of the VPS1 gene of S. cerevisiae which encodes a dynamin-related protein. The vpsA gene encodes a polypeptide consisting of 696 amino acids that is nearly 60% homologous to the S. cerevisiae Vps1. Similar to Vps1, VpsA contains a highly conserved tripartite GTPase domain but lacks the pleckstrin homology domain and proline-rich region. The vpsA disruptant shows poor growth and contains highly fragmented vacuoles. These results suggest that A. nidulans VpsA functions in the vacuolar biogenesis.
authors.affiliation: Department of Biotechnology, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, 113-8657, Tokyo, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Tarutani
authors.forename: K
authors.initials: K
authors.lastname: Ohsumi
authors.forename: M
authors.initials: M
authors.lastname: Arioka
authors.forename: H
authors.initials: H
authors.lastname: Nakajima
authors.forename: K
authors.initials: K
authors.lastname: Kitamoto
mesh_terms: D000595:Amino Acid Sequence; D001233:Aspergillus nidulans / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D001426:Bacterial Proteins; D001483:Base Sequence; D001665:Binding Sites; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D002455:Cell Division / Q000235:genetics; D003001:Cloning, Molecular; D034281:Dynamins; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D020558:GTP Phosphohydrolases / Q000235:genetics* / Q000378:metabolism; D019204:GTP-Binding Proteins; D005816:Genetic Complementation Test; D006160:Guanosine Triphosphate / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D017386:Sequence Homology, Amino Acid; D014617:Vacuoles / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D033921:Vesicular Transport Proteins
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D005656:Fungal Proteins; D033921:Vesicular Transport Proteins; C114922:VpsA protein, Shigella flexneri; D006160:Guanosine Triphosphate; D020558:GTP Phosphohydrolases; D019204:GTP-Binding Proteins; C064553:VPS1 protein, S cerevisiae; C431138:VpsA protein, Aspergillus nidulans; D034281:Dynamins