Effect of lectin from Chelidonium majus L. on normal and cancer cells in culture.
Lectin from Chelidonium majus L. (CML) significantly stimulates the proliferation of human lymphocytes and has hemagglutination activity towards group B human erythrocytes and potent antimicrobial properties against multiresistant enterococci and staphylococci. In the present work we describe the effect of lectin from Chelidonium majus L on normal and cancercells in culture in vitro. The studies were performed on three types of cells: CHO, R2C and on normal mouse fibroblasts. Effects on the cultures were examined 24 h after addition of CML. Exposure to CML resulted in growth inhibition of CHO and R2C cells but not of fibroblasts. Moreover, evident apoptotic lesions were observed in CHO cells and less well marked apoptotic lesions in R2C cells. In contrast, only insignificant numbers of fibroblasts reacted to the applied lectin.
Fik
E
E
Department of Molecular Virology, University of Poznań, Poland.
Wołuń-Cholewa
M
M
Kistowska
M
M
Warchoł
J B
JB
Goździcka-Józefiak
A
A
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D016466:CHO Cells; D002851:Chromatography, High Pressure Liquid; D006224:Cricetinae; D004247:DNA / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D005347:Fibroblasts; D037102:Lectins / Q000737:chemistry* / Q000494:pharmacology*; D051379:Mice; D010207:Papaver / Q000737:chemistry*; D037121:Plant Lectins; D010946:Plants, Medicinal; D013056:Spectrophotometry, Ultraviolet; D014407:Tumor Cells, Cultured
D000972:Antineoplastic Agents, Phytogenic; D037102:Lectins; D037121:Plant Lectins; D004247:DNA