title: Enzyme-assisted preparation of isotope-labeled 1-deoxy-d-xylulose 5-phosphate.
abstract: Recombinant 1-deoxy-D-xylulose 5-phosphate synthase of Bacillus subtilis was used for the preparation of isotope-labeled 1-deoxy-D-xylulose 5-phosphate using isotope-labeled glucose and/or isotope-labeled pyruvate as starting materials. The simple one-pot methods described afford almost every conceivable isotopomer of 1-deoxy-D-xylulose 5-phosphate carrying (13)C or (14)C from commercially available precursors with an overall yield around 50%.
authors.affiliation: Lehrstuhl für Organische Chemie und Biochemie, Technische Universität München, Lichtenbergstr. 4, D-85747 Garching, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Hecht
authors.forename: K
authors.initials: K
authors.lastname: Kis
authors.forename: W
authors.initials: W
authors.lastname: Eisenreich
authors.forename: S
authors.initials: S
authors.lastname: Amslinger
authors.forename: J
authors.initials: J
authors.lastname: Wungsintaweekul
authors.forename: S
authors.initials: S
authors.lastname: Herz
authors.forename: F
authors.initials: F
authors.lastname: Rohdich
authors.forename: A
authors.initials: A
authors.lastname: Bacher
mesh_terms: D001412:Bacillus subtilis / Q000201:enzymology; D007202:Indicators and Reagents; D007553:Isotope Labeling; D009682:Magnetic Resonance Spectroscopy; D010428:Pentosephosphates / Q000138:chemical synthesis*; D010957:Plasmids; D011994:Recombinant Proteins / Q000378:metabolism; D013237:Stereoisomerism; D014166:Transferases / Q000378:metabolism*
chemical_list: C109460:1-deoxylulose 5-phosphate; D007202:Indicators and Reagents; D010428:Pentosephosphates; D011994:Recombinant Proteins; D014166:Transferases; C109461:deoxyxylulose-5-phosphate synthase