title: In vivo expression profiles of cytokine and iNOS mRNAs in rats infected with Eimeria separata.
abstract: Studies on cytokine (IFN-gamma, IL-2, IL-4, IL-5, IL-10) and inducible NO-synthase (iNOS) gene transcription in mesenteric lymph nodes (MLN) and the caecum wall were performed 0, 48, and 72h after primary and challenge infections of rats with Eimeria separata using RT-PCR. The amount of IFN-gamma mRNA was elevated in MLN and caeca 72h after primary and 48-72h after challenge infection when compared with uninfected controls. Increased amounts of IL-2 mRNA were only found in MLN of infected rats 72h post-infection (p.i.). In case of IL-10, infections did not affect the amount of mRNA in MLN, but led to markedly increased levels in the caecum wall of both infected groups 48 and 72h p.i. Levels of IL-4 mRNA remained unchanged after infections and IL-5 gene transcripts were undetectable. Amounts of iNOS mRNA (not investigated in MLN) were found strongly enhanced 48 and 72h p.i. in the caecum walls of all infected animals when compared with naive controls. The data are discussed in regard of the cellular source of the cytokines and their immunological role.
authors.affiliation: Institute of Parasitology, Justus Liebig University Giessen, Rudolf-Buchheim-Strasse 2, D-35392 Giessen, Germany.
authors.forename: M Q
authors.initials: MQ
authors.lastname: Shi
authors.forename: J
authors.initials: J
authors.lastname: Hirzmann
authors.forename: T H
authors.initials: TH
authors.lastname: Dafa'alla
authors.forename: H
authors.initials: H
authors.lastname: Zahner
mesh_terms: D000818:Animals; D002432:Cecum / Q000469:parasitology; D003048:Coccidiosis / Q000378:metabolism / Q000662:veterinary*; D016207:Cytokines / Q000096:biosynthesis* / Q000235:genetics; D004539:Eimeria; D007371:Interferon-gamma / Q000096:biosynthesis / Q000235:genetics; D016753:Interleukin-10 / Q000096:biosynthesis / Q000235:genetics; D007376:Interleukin-2 / Q000096:biosynthesis / Q000235:genetics; D015847:Interleukin-4 / Q000096:biosynthesis / Q000235:genetics; D015848:Interleukin-5 / Q000096:biosynthesis / Q000235:genetics; D008297:Male; D019001:Nitric Oxide Synthase / Q000096:biosynthesis* / Q000235:genetics; D052247:Nitric Oxide Synthase Type II; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D011917:Rats, Inbred Lew; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D012376:Rodent Diseases / Q000378:metabolism* / Q000469:parasitology
chemical_list: D016207:Cytokines; D007376:Interleukin-2; D015848:Interleukin-5; D012333:RNA, Messenger; D016753:Interleukin-10; D015847:Interleukin-4; D007371:Interferon-gamma; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496318:Nos2 protein, rat