title: The ORFO product of Potato leafroll virus is indispensable for virus accumulation.
abstract: Using a cDNA expression cassette in combination with agroinoculation of potato leaf discs we have investigated the role the protein encoded by ORF0 of Potato leafroll virus (PLRV) and have shown its importance for virus accumulation. Two mutations introduced into ORF0 by site-directed mutagenesis prevented expression of the corresponding protein and completely abolished virus accumulation in plant cells. They did not, however, affect translation of ORF1 and ORF2. We therefore conclude that ORF0 of PLRV produces a protein essential for virus accumulation, a hitherto undescribed finding.
authors.affiliation: Institut des Sciences Végétales CNRS, Av. de la Terrasse, 91 198 Gif-sur-Yvette, France2.|Institute of Biochemistry and Biophysics PAS, Ul. Pawińskiego 5A, 02-106 Warsaw, Poland1.
authors.forename: Ewa
authors.initials: E
authors.lastname: Sadowy
authors.affiliation: Institute of Biochemistry and Biophysics PAS, Ul. Pawińskiego 5A, 02-106 Warsaw, Poland1.
authors.forename: Anna
authors.initials: A
authors.lastname: Maasen
authors.affiliation: Institute of Biochemistry and Biophysics PAS, Ul. Pawińskiego 5A, 02-106 Warsaw, Poland1.
authors.forename: Marek
authors.initials: M
authors.lastname: Juszczuk
authors.affiliation: Institut des Sciences Végétales CNRS, Av. de la Terrasse, 91 198 Gif-sur-Yvette, France2.
authors.forename: Chantal
authors.initials: C
authors.lastname: David
authors.affiliation: Institute of Biochemistry and Biophysics PAS, Ul. Pawińskiego 5A, 02-106 Warsaw, Poland1.
authors.forename: Wlodzimierz
authors.initials: W
authors.lastname: Zagórski-Ostoja
authors.affiliation: Institut des Sciences Végétales CNRS, Av. de la Terrasse, 91 198 Gif-sur-Yvette, France2.
authors.forename: Bruno
authors.initials: B
authors.lastname: Gronenborn
authors.affiliation: Institute of Biochemistry and Biophysics PAS, Ul. Pawińskiego 5A, 02-106 Warsaw, Poland1.
authors.forename: M Danuta
authors.initials: MD
authors.lastname: Hulanicka
mesh_terms: D020043:Genes, Essential / Q000235:genetics; D017793:Luteovirus / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D016297:Mutagenesis, Site-Directed / Q000235:genetics; D016366:Open Reading Frames / Q000235:genetics; D018515:Plant Leaves / Q000166:cytology / Q000821:virology; D014176:Protein Biosynthesis; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism; D011198:Solanum tuberosum / Q000166:cytology / Q000821:virology*; D014764:Viral Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D014779:Virus Replication / Q000235:genetics
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins