title: An immunohistochemical study of adenohypophyseal cells in the viviparous reptile Chalcides chalcides.
abstract: The morphology of the hypophysis and the immunocharacteristics of the adenohypophyseal cells in the viviparous reptile Chalcides chalcides were studied by light microscopy, using conventional staining methods and an indirect antibody technique (ABC method), respectively. The general morphology of the C. chalcides hypophysis was comparable to that of other reptiles, showing three main regions: the pars distalis, the pars intermedia and the pars nervosa. The gland appeared as an elongated body in a cephalic-caudal direction and was almost completely enclosed in the sella turcica. For this reason, the hypophysis was studied in toto with the brain in decalcified specimens. The pars distalis accounted for most of the whole organ. The pars intermedia surrounded the pars nervosa as a goblet. The pars tuberalis was lacking. The immunohistochemical identification of the adenohypophyseal cells was performed using rabbit antisera against mammalian/synthetic hypophyseal hormones. Prolactin cells were clustered in small cellular cordons in the rostral pars distalis and in the medial pars distalis in both male and female specimens. Somatotropic cells were found in the caudal pars distalis. Corticotropic cells were observed in the medio-rostral pars distalis, as well as in the pars intermedia, where melanotropic cells were also present. Melanotropic cells were confined to the pars intermedia. Gonadotropic cells were mostly distributed in the ventral and lateral portions of the pars distalis, where they were found isolated or in small clusters. Thyrotropic cells were detected in the pars distalis with a distribution similar to that of the gonadotropic cells; however, atypically, they were also found in the pars intermedia. Therefore, the cytological characteristics of the adenohypophyseal cells appeared mostly conserved.
authors.affiliation: Department of Evolutionary and Comparative Biology, University of Naples Federico II, Italy.
authors.forename: I
authors.initials: I
authors.lastname: Ferrandino
authors.forename: G
authors.initials: G
authors.lastname: Viscardi
authors.forename: M C
authors.initials: MC
authors.lastname: Grimaldi
mesh_terms: D000818:Animals; D005260:Female; D007124:Immunoenzyme Techniques; D008116:Lizards / Q000033:anatomy & histology*; D008297:Male; D010503:Periodic Acid-Schiff Reaction; D010902:Pituitary Gland / Q000737:chemistry / Q000166:cytology*; D010908:Pituitary Hormones, Anterior / Q000032:analysis; D013194:Staining and Labeling
chemical_list: D010908:Pituitary Hormones, Anterior