[Hepatitis C virus culture systems].
Hepatitis C virus pathogenesis and cycle are difficult to study because of the lack of culture system able to replicate efficiently the virus. Furthermore such a system will permit screen new antiviral drugs. Studies were realized to select cell culture system able to allow hepatitis C virus replication. Primary cell cultures and cell lines were used to performed HCV culture. Most of these works used lymphocyte and hepatocyte primary cultures or cell lines because of HCV tropism in these cells in vivo. Animals and arthropods cell lines were used as well for their capacity to bind and replicate HCV. The aim of this review is to present the different cell systems used to replicate HCV in culture and the results obtained.
Germi
R
R
Laboratoire de virologie moléculaire et structurale, faculté de médecine/pharmacie de Grenoble, France. raphaele.germi@ujf-grenoble.fr
Crance
J M
JM
Garin
D
D
Zarski
J P
JP
Drouet
E
E
D000330:Aedes / Q000166:cytology; D000818:Animals; D001402:B-Lymphocytes / Q000821:virology; D002415:Cats; D018929:Cell Culture Techniques / Q000379:methods; D002478:Cells, Cultured / Q000821:virology; D002522:Chlorocebus aethiops; D006224:Cricetinae; D004847:Epithelial Cells / Q000821:virology; D005347:Fibroblasts / Q000821:virology; D005704:Gallbladder / Q000166:cytology; D016174:Hepacivirus / Q000254:growth & development* / Q000502:physiology; D022781:Hepatocytes / Q000821:virology; D006801:Humans; D007668:Kidney / Q000166:cytology; D007963:Leukocytes, Mononuclear / Q000821:virology; D009928:Organ Specificity; D013601:T-Lymphocytes / Q000821:virology; D014407:Tumor Cells, Cultured / Q000821:virology; D014776:Virus Cultivation / Q000379:methods*; D014779:Virus Replication