title: Induction of aneuploidy by nickel sulfate in V79 Chinese hamster cells.
abstract: The ability of nickel sulfate (NiSO(4)) to induce chromosome aneuploidy was investigated in vitro using the V79 Chinese hamster cell line. V79 cells were treated with 100-400 microM NiSO(4) for 24h, and monitored up to 72 h following treatment with a chromosome aberration assay, a micronuclei assay using antikinetochore antibodies (CREST assay) and an anaphase/telophase assay. Aneuploid cells were induced in a significant fraction of the cell population 24-48 h following treatment with nickel sulfate. The majority of these cells were hyperdiploid. In addition, nickel sulfate caused increased frequency of cells with kinetochore-positive micronuclei as well as kinetochore-negative micronuclei. Abnormal chromosome segregation such as lagging chromosomes, chromosome bridges and asymmetric segregation were also observed in more than 50% of anaphase or telophase cells following treatment with NiSO(4). The incidences of these abnormalities were dose-dependent in general, although the effects were prominent in a sublethal dose. These results indicate that NiSO(4) has the ability to induce aneuploidy in V79 cells. In addition, the results in anaphase/telophase assay suggest that the compound may have an effect on spindle apparatus, which could result in aneuploidy following abnormal chromosome segregation.
authors.affiliation: Division of Morphological Science, Biomedical Research Center, Saitama Medical School, 38 Morohongo, Moroyama, Iruma, 350-0495, Saitama, Japan. sohshima@saitama-med.ac.jp
authors.forename: S
authors.initials: S
authors.lastname: Ohshima
mesh_terms: D000705:Anaphase / Q000187:drug effects; D000782:Aneuploidy; D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002875:Chromosomes / Q000187:drug effects / Q000276:immunology; D006224:Cricetinae; D003412:Cricetulus; D004305:Dose-Response Relationship, Drug; D007621:Karyotyping; D018386:Kinetochores / Q000187:drug effects / Q000276:immunology; D015162:Micronucleus Tests; D009153:Mutagens / Q000633:toxicity*; D009532:Nickel / Q000633:toxicity*; D013692:Telophase / Q000187:drug effects
chemical_list: D009153:Mutagens; C029938:nickel sulfate; D009532:Nickel