Quantitative two-color fluorescence cross-correlation spectroscopy in the analysis of polymerase chain reaction.
We present results of an approach in which low-density labeled DNA itself provides an amplification of the cross-correlated fluorescent signal in the two-color cross-correlation function. Tetramethylrhodamine-4-dUTP and Cy5-dCTP are incorporated by polymerase chain reaction at multiple positions of the same 217 bp target DNA. We call this novel approach the 'two-color FCS signal amplification'. The signal amplification is an example for interactions of two ligands with different colors at multiple positions of the same target.
Földes-Papp
Z
Z
Department of Medical Biophysics, MBB, Karolinska Institute, Stockholm, Sweden. zeno.foldes-papp@kfunigraz.ac.at
Rigler
R
R
D002232:Carbocyanines / Q000737:chemistry; D004247:DNA / Q000737:chemistry; D003856:Deoxyuracil Nucleotides / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D008962:Models, Theoretical; D016133:Polymerase Chain Reaction / Q000379:methods*; D012235:Rhodamines / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000379:methods*
C088942:3'-deoxy-5-(cyanine dye 5)uridine 5'-trisphosphate; D002232:Carbocyanines; D003856:Deoxyuracil Nucleotides; D005456:Fluorescent Dyes; D012235:Rhodamines; C027078:deoxyuridine triphosphate; C005358:tetramethylrhodamine; D004247:DNA