title: Cellular and humoral immune response in progressive multifocal leukoencephalopathy.
abstract: Progressive multifocal leukoencephalopathy (PML) is a fatal, demyelinating disease caused by JC virus (JCV) in patients with severe immunosuppression. We studied the JCV-specific cellular and humoral immune response in 7 healthy donors (HD), 6 human immunodeficiency virus-1 (HIV-1)-infected patients without PML (HIV), 4 HIV-1-negative patients with PML (PML), and 8 HIV-1-positive patients with PML (HIV/PML). As antigens, recombinant virus-like particles of the major structural protein VP1 (VP1-VLP) of JCV, tetanus toxoid (TT), or the mitogen phytohemagglutinin (PHA) were used. Proliferation of peripheral blood mononuclear cells (PBMC) after stimulation with the VP1-VLP was significantly suppressed in PML and HIV/PML patients compared to HD. After antigen stimulation the production of interferon-gamma (IFN-gamma) was reduced in PML, in HIV/PML, and in HIV patients. The production of interleukin-10 (IL-10), however, was elevated in HIV/PML patients. Neither proliferation nor cytokine production correlated with the presence of JCV DNA in PBMC. The immunoglobulin G serum antibody titer to the VP1-VLP was slightly elevated in HIV, elevated in PML, and highly elevated in HIV/PML patients compared to HD. The development of PML appears to coincide with a general impairment of the Th1-type T-helper cell function of cell-mediated immunity.
authors.affiliation: Department of Neurology, University of Göttingen, Germany. fweber@mpipsykl.mpg.de
authors.forename: F
authors.initials: F
authors.lastname: Weber
authors.forename: C
authors.initials: C
authors.lastname: Goldmann
authors.forename: M
authors.initials: M
authors.lastname: Krämer
authors.forename: F J
authors.initials: FJ
authors.lastname: Kaup
authors.forename: M
authors.initials: M
authors.lastname: Pickhardt
authors.forename: P
authors.initials: P
authors.lastname: Young
authors.forename: H
authors.initials: H
authors.lastname: Petry
authors.forename: T
authors.initials: T
authors.lastname: Weber
authors.forename: W
authors.initials: W
authors.lastname: Lüke
mesh_terms: D016207:Cytokines / Q000276:immunology; D015658:HIV Infections / Q000276:immunology*; D015497:HIV-1; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D007577:JC Virus / Q000276:immunology*; D007963:Leukocytes, Mononuclear / Q000276:immunology; D007968:Leukoencephalopathy, Progressive Multifocal / Q000276:immunology*; D016133:Polymerase Chain Reaction; D015678:Viral Structural Proteins / Q000276:immunology*
chemical_list: D016207:Cytokines; D007074:Immunoglobulin G; D015678:Viral Structural Proteins