H+,K+-ATPase is not involved in endolymph pH homeostasis.
In endolymph, the calculated electrochemical gradients suggest the presence of active transport of H+ and K+. The presence of H+,K+-ATPase, an active transporter that mediates the exchange of intracellular H+ for extracellular K+, was investigated using reverse transcription-polymerase chain reaction on rat microdissected inner ear tissues. Distal colon and kidney were used as positive controls. The two H+,K+-ATPase colonic or gastric isoforms were not detected in the rat cochlea and endolymphatic sac. This result suggests that H+,K+-ATPase is not involved in H+ and K+ endolymph homeostasis.
Couloigner
V
V
INSERM U.426, Faculté Xavier Bichat, Paris, France.
Teixeira
M
M
Bozorg-Grayeli
A
A
Friedlander
G
G
Sterkers
O
O
Ferrary
E
E
D000136:Acid-Base Equilibrium / Q000502:physiology*; D000818:Animals; D004710:Endolymph / Q000502:physiology*; D004712:Endolymphatic Sac / Q000502:physiology; D006706:Homeostasis / Q000502:physiology*; D006863:Hydrogen-Ion Concentration; D008297:Male; D051381:Rats; D020318:Rats, Long-Evans; D000254:Sodium-Potassium-Exchanging ATPase / Q000502:physiology*
D000254:Sodium-Potassium-Exchanging ATPase