Fluorometric determination of EDTA and EGTA using terbium-salicylate complex.
A method for determining EDTA (ethylenediaminetetraacetic acid) based on a highly fluorescent terbium-EDTA-salicylic acid complex formation was developed. EDTA from as low as a few picomoles to as high as several nanomoles can be determined in a microtiter plate in 10-20 min. Ethyleneglycol-bis(2-aminoethoxy)-tetraacetic acid (EGTA) also can be determined by the same method, but its sensitivity is ca. 14-fold lower. Interestingly, diethylenetriamine tetraacetic acid did not form fluorescent complex with terbium under the same conditions.
Lee
Y C
YC
Biology Department, Johns Hopkins University, 3400 North Charles Street, Baltimore, MD 21218, USA. yclee@jhu.edu
D002118:Calcium / Q000032:analysis; D004492:Edetic Acid / Q000032:analysis*; D004533:Egtazic Acid / Q000032:analysis*; D005470:Fluorometry / Q000379:methods*; D004369:Pentetic Acid / Q000378:metabolism; D012459:Salicylates / Q000032:analysis / Q000378:metabolism*; D013725:Terbium / Q000378:metabolism*
D012459:Salicylates; D013725:Terbium; D004533:Egtazic Acid; D004369:Pentetic Acid; D004492:Edetic Acid; D002118:Calcium