A scanning near-field optical microscope approach to biomolecule patterning.
In view of future generations of biosensors and advanced biomaterials, photochemistry in the near field using scanning near-field optical microscopy is investigated. The potential of direct near-field-induced photoactivation is demonstrated on standard photoresist. Photoimmobilization of maleimidoaryldiazirine on silicon substrates and bovine serum albumin on glass substrates is achieved, opening the way to a controlled biopatterning of surfaces with submicrometer feature size. The obtained patterns are characterized using atomic force microscopy, time-of-flight secondary ion mass spectroscopy (ToF-SIMS), and near-field fluorescence microscopy.
Philipona
C
C
Institut d'Optique Appliquée, Ecole polytechnique Fédérale de Lausanne (EPFL), CH-1015 Lausanne, Switzerland.
Chevolot
Y
Y
Léonard
D
D
Mathieu
H J
HJ
Sigrist
H
H
Marquis-Weible
F
F
D000327:Adsorption; D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D002417:Cattle; D018625:Microscopy, Atomic Force; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D010777:Photochemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism; D018629:Spectrometry, Mass, Secondary Ion; D013499:Surface Properties; D014159:Transducers
D012710:Serum Albumin, Bovine