Cell contact dependent extended release of adenovirus by microparticles in vitro.
Adenoviral vectors remain one of the most promising methods of gene delivery but are plagued by several inherent problems including immune inactivation and transient expression. This paper reports a novel microparticle-based delivery system for adenovirus that allows high uptake of virus, stable complex formation and extended release. In addition, this microparticle/adenovirus complex has been demonstrated to only release virus upon cell contact. The significant clinical implications of this delivery system are discussed.
Cavanagh
H M
HM
School of Biomedical Sciences, Charles Sturt University, Locked Bag 588, NSW 2678, Wagga Wagga, Australia. hcavanagh@csu.edu.au
Dingwall
D
D
Steel
J
J
Benson
J
J
Burton
M
M
D000260:Adenoviruses, Human / Q000276:immunology / Q000502:physiology*; D002460:Cell Line; D006801:Humans; D014407:Tumor Cells, Cultured