title: Glut-1 expression in dysplastic and regenerative lesions of the colon.
abstract: Monosaccaride transporter proteins are responsible for transmembrane transport of monosaccarides into cells. Glucose transporter protein 1 (Glut-1) is most prevalent in the cell membranes of erythrocytes and facilitates transport of glucose in tissues with barrier functions, i.e. blood brain barrier. Expression of Glut-1 in malignant tumors is increased due to increased metabolic need of the proliferating cell populations. In colorectal adenomas and carcinomas, membranous expression of Glut-1 has been associated with higher grade of tumors and decreased survival time. We studied the expression of Glut-1 in dysplastic proliferations of the colon which included sporadic adenomas and dysplasia associated lesions (DALM) in patients with ulcerative colitis and reactive/regenerative proliferations of the colon, including non-dysplastic chronic colitis, acute colitis and ischemia. Two patterns of Glut-1 expression were detected. Most adenomas and DALMs showed at least focal membranous expression of Glut-1. In addition a second staining pattern was recognized which consisted of prominent supranuclear dots. This pattern of staining was not only seen in adenomas and DALM but also in non-dysplastic areas immediately surrounding sporadic adenomas, in regenerative chronic colitis and in areas surrounding acute inflammation. Areas away from dysplasia did not show any positive staining for Glut-1. We conclude that two distinct patterns of Glut-1 expression may be found in colonic epithelial proliferation: membranous staining, associated with dysplasia, and, heretofore not described, supranuclear staining which may be related to Glut-1 expression secondary to expression of specific growth factors and not necessarily related to dysplasia.
authors.affiliation: Department of Pathology, University of Pennsylvania, Presbyterian Medical Center, Philadelphia, PA 19104, USA.
authors.forename: F
authors.initials: F
authors.lastname: Fogt
authors.forename: A
authors.initials: A
authors.lastname: Wellmann
authors.forename: S J
authors.initials: SJ
authors.lastname: Urbanski
authors.forename: A
authors.initials: A
authors.lastname: Noffsinger
authors.forename: C
authors.initials: C
authors.lastname: Poremba
authors.forename: R L
authors.initials: RL
authors.lastname: Zimmerman
authors.forename: N
authors.initials: N
authors.lastname: Alsaigh
mesh_terms: D000236:Adenoma / Q000378:metabolism / Q000473:pathology; D002455:Cell Division; D002467:Cell Nucleus / Q000378:metabolism; D003092:Colitis / Q000378:metabolism / Q000473:pathology; D003106:Colon / Q000378:metabolism* / Q000473:pathology; D003108:Colonic Diseases / Q000378:metabolism* / Q000473:pathology; D003110:Colonic Neoplasms / Q000378:metabolism* / Q000473:pathology; D003424:Crohn Disease / Q000378:metabolism / Q000473:pathology; D051272:Glucose Transporter Type 1; D006801:Humans; D007511:Ischemia / Q000378:metabolism / Q000473:pathology; D009004:Monosaccharide Transport Proteins / Q000096:biosynthesis*
chemical_list: D051272:Glucose Transporter Type 1; D009004:Monosaccharide Transport Proteins; C495677:SLC2A1 protein, human