Enhancing the neuronal interaction on fluoropolymer surfaces with mixed peptides or spacer group linkers.
Embryonic hippocampal neurons cultured on surface modified fluoropolymers showed enhanced interaction and neurite extension. Poly(tetrafluoroethylene-co-hexafluoropropylene) (FEP) film surfaces were aminated by reaction with a UV-activated mercury ammonia system yielding FEP-[N/O]. Laminin-derived cell-adhesive peptides (YIGSR and IKVAV) were coupled to FEP surface functional groups using tresyl chloride activation. Embryonic (E18) hippocampal neurons were cultured in serum-free medium for up to 1 week on FEP film surfaces that were modified with either one or both of GYIGSR and SIKVAV or GGGGGGYIGSR and compared to control surfaces of FEP-[N/O] and poly(L-lysine)/laminin-coated tissue culture polystyrene. Neuron-surface interactions were analyzed over time in terms of neurite outgrowth (number and length of neurites), cell adhesion and viability. Neurite outgrowth and adhesion were significantly better on peptide-modified surfaces than on either FEP or FEP-[N/O]. Cells on the mixed peptide (GYIGSR/SIKVAV) and the spacer group peptide (GGGGGGYIGSR) surfaces demonstrated similar behavior to those on the positive PLL/laminin control. The specificity of the cell-peptide interaction was demonstrated with a competitive assay where dissociated neurons were incubated in media containing peptides prior to plating. Cell adhesion and neurite outgrowth diminished on all surfaces when hippocampal neurons were pre-incubated with dissolved peptides prior to plating.
Tong
Y W
YW
Department of Chemical Engineering and Applied Chemistry, University of Toronto, Ont., Canada.
Shoichet
M S
MS
D000595:Amino Acid Sequence; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002450:Cell Communication; D002478:Cells, Cultured; D004622:Embryo, Mammalian / Q000166:cytology; D006624:Hippocampus / Q000166:cytology; D007797:Laminin / Q000737:chemistry; D008422:Materials Testing; D016501:Neurites / Q000648:ultrastructure; D009474:Neurons / Q000166:cytology*; D009842:Oligopeptides / Q000737:chemistry; D011138:Polytetrafluoroethylene / Q000031:analogs & derivatives; D051381:Rats; D013499:Surface Properties; D005465:Fluorocarbon Polymers
D001672:Biocompatible Materials; D007797:Laminin; D009842:Oligopeptides; D011138:Polytetrafluoroethylene; C096305:fluorinated ethylene propylene; D005465:Fluorocarbon Polymers