Determination of inorganic cations and anions by ion-exchange chromatography with evaporative light-scattering detection.
Evaporative light-scattering detection (ELSD) was investigated for the direct determination of alkali and alkaline-earth cations by cation-exchange chromatography. Successful single run analysis of Na+, K+, Mg2+ and Ca2+ was achieved in 11 min on the Hamilton PRP-X200 column using an aqueous solution of ammonium formate as mobile phase under a salt concentration step gradient mode (20 mM and 100 mM). Surprisingly the use of ELSD reveals a weak retention of inorganic anions (Cl-, NO3-, SO4(2-)) onto the polymeric cation exchanger, which enables the simultaneous determination of inorganic anions (C1- and NO3-) associated with the cations analysed (Na+ and K+).
Mouchère
F
F
Institut de Chimie Organique & Analytique, Université d'Orleans, UMR 6005, France.
El Haddad
M
M
Elfakir
C
C
Dreux
M
M
D000838:Anions / Q000032:analysis; D002412:Cations / Q000032:analysis; D002852:Chromatography, Ion Exchange / Q000379:methods*; D008027:Light; D008670:Metals / Q000032:analysis* / Q000737:chemistry; D012542:Scattering, Radiation
D000838:Anions; D002412:Cations; D008670:Metals