[Inhibitory effect of genistein on the proliferation of the anterior pituitary cells of rats].
The effects of tyrosine kinase inhibitor genistein on the proliferation of rat anterior pituitary cells and mouse AtT-20 cells were studied using techniques of cell culture, 3H-TdR incorporation, flow cytometric analysis and electron microscope. Genistein significantly inhibited the proliferation of rat anterior pituitary cells and mouse AtT-20 cells. Genistein (50 and 100 mumol/L) blocked the proliferation of AtT-20 cells at G0/G1 and G2/M phases and evoked an apoptotic peak of these cells with an apoptotic ratio of 19.9% and 36.4%. The apoptotic cells were also observed under the electron microscope. In consequence, genistein, as a tyrosine kinase inhibitor, can significantly inhibit the proliferation of pituitary cells possibly by inducing apoptosis, and the tyrosine kinase activity may play a key role in the proliferation and differentiation of pituitary cells.
Zhang
Q H
QH
Department of Physiology, Fourth Military Medical University, Xi'an 710032. qinghong@fmmu.edu.cn
Hu
Y Z
YZ
Zhou
S S
SS
Wang
F Z
FZ
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D005260:Female; D019833:Genistein / Q000494:pharmacology*; D010903:Pituitary Gland, Anterior / Q000166:cytology*; D010911:Pituitary Neoplasms / Q000473:pathology; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014407:Tumor Cells, Cultured / Q000187:drug effects
D019833:Genistein; D011505:Protein-Tyrosine Kinases