title: HIV Rev peptides conjugated with peptide nucleic acids and their efficient binding to RRE RNA.
abstract: HIV Rev peptides conjugated with peptide nucleic acids (PNAs) were designed and synthesized to develop a designing approach for a novel RNA-binding molecule. The binding affinities of PNA-peptides with the Rev responsive element (RRE) RNA were determined by the competition assay using a rhodamine-labeled Rev. The peptide conjugated with an antisense PNA (TGCGC) bound RRE RNA more efficiently than the molecule without the PNA or the peptide sequence.
authors.affiliation: Department of Bioengineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta, Yokohama, Japan.
authors.forename: I
authors.initials: I
authors.lastname: Kumagai
authors.forename: T
authors.initials: T
authors.lastname: Takahashi
authors.forename: K
authors.initials: K
authors.lastname: Hamasaki
authors.forename: A
authors.initials: A
authors.lastname: Ueno
authors.forename: H
authors.initials: H
authors.lastname: Mihara
mesh_terms: D000595:Amino Acid Sequence; D016880:Anisotropy; D002942:Circular Dichroism; D015497:HIV-1 / Q000737:chemistry*; D008969:Molecular Sequence Data; D020135:Peptide Nucleic Acids / Q000737:chemistry*; D012367:RNA, Viral / Q000737:chemistry* / Q000378:metabolism; D020218:Response Elements / Q000187:drug effects*; D013050:Spectrometry, Fluorescence
chemical_list: D020135:Peptide Nucleic Acids; D012367:RNA, Viral