title: Effects of common topical otic preparations on the morphology of isolated cochlear outer hair cells.
abstract: Otic drops are commonly used not only for otitis externa but also for otorrhea in the presence of tympanostomy tube or tympanic membrane perforation. Many studies have demonstrated the ototoxicity of common otic preparations such as Cortisporin otic drops. Recent studies have suggested the use of fluoroquinolone antibiotic drops as an alternative owing to their excellent antimicrobial coverage and no ototoxic effect. The purpose of this study was to assess the relative ototoxicity of four common otic preparations by direct exposure to isolated cochlear outer hair cells (OHCs). OHCs from adult chinchilla cochlea were exposed to standard bathing solution (control), Cortisporin, Cipro HC, Ciloxan, and Floxin. The cells were observed using an inverted microscope, and the images recorded in digital still-frame and video, and analyzed on the Image Pro-Plus 3.0 program. As measured by time to cell death and change in morphology of OHCs, Cortisporin was most toxic to OHCs. Among the fluoroquinolone drops, Floxin was more toxic than Ciloxan or Cipro HC.
authors.affiliation: Division of Otolaryngology, Head and Neck Surgery, Loma Linda University School of Medicine, CA, USA.
authors.forename: P T
authors.initials: PT
authors.lastname: Russell
authors.forename: C A
authors.initials: CA
authors.lastname: Church
authors.forename: T H
authors.initials: TH
authors.lastname: Jinn
authors.forename: D J
authors.initials: DJ
authors.lastname: Kim
authors.forename: E O
authors.initials: EO
authors.lastname: John
authors.forename: T T
authors.initials: TT
authors.lastname: Jung
mesh_terms: D000287:Administration, Topical; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002682:Chinchilla; D002939:Ciprofloxacin / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations; D018072:Hair Cells, Auditory, Outer / Q000187:drug effects*; D006854:Hydrocortisone / Q000633:toxicity*; D009355:Neomycin / Q000633:toxicity*; D015242:Ofloxacin / Q000633:toxicity*; D011112:Polymyxin B / Q000633:toxicity*
chemical_list: D004338:Drug Combinations; C033225:hydrocortisone, neomycin, polymyxin B drug combination; D002939:Ciprofloxacin; D015242:Ofloxacin; D009355:Neomycin; D011112:Polymyxin B; D006854:Hydrocortisone