Ruscogenin glycoside (Lm-3) isolated from Liriope muscari improves liver injury by dysfunctioning liver-infiltrating lymphocytes.
The effects of ruscogenin 1-O-[beta-D-glucopyranosyl(1 --> 2)] [beta-D-xylopyranosyl(1 --> 3)]-beta-D-fucopyranoside (Lm-3) and its aglycone, ruscogenin, on liver injury induced in mice by delayed-type hypersensitivity to picryl chloride have been investigated. Lm-3 and ruscogenin significantly decreased liver injury when given during the effector phase of the delayed-type hypersensitivity reaction. The pretreatment of nonparenchymal cells, but not hepatocytes, with Lm-3 or ruscogenin in-vitro caused a concentration- and time-dependent inhibition against the damage. Lm-3 showed a stronger inhibition against the damage than ruscogenin (IC50: Lm-3 6.3 x 10(-10) M, ruscogenin 3.9 x 10(-7) M). However, neither Lm-3 nor ruscogenin blocked the hepatotoxic potential of CCl4, when used to pretreat hepatocytes. Moreover, Lm-3 and ruscogenin inhibited concanavalin A-induced lymphocyte proliferation only at high concentrations. These results suggested that Lm-3 and ruscogenin improved the immunological liver injury by selectively causing dysfunction of the liver-infiltrating cells rather than by protecting hepatocyte membranes. Such characteristics would be significant for treating immunologically related liver diseases as well as for developing new drugs.
Wu
F
F
Department of Pharmacology for Chinese Materia Medica, China Pharmaceutical University, Nanjing.
Cao
J
J
Jiang
J
J
Yu
B
B
Xu
Q
Q
D000818:Animals; D004305:Dose-Response Relationship, Drug; D005260:Female; D006027:Glycosides; D022781:Hepatocytes / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D008099:Liver / Q000187:drug effects / Q000276:immunology / Q000473:pathology*; D008107:Liver Diseases / Q000276:immunology / Q000503:physiopathology* / Q000517:prevention & control; D008214:Lymphocytes / Q000187:drug effects*; D051379:Mice; D008813:Mice, Inbred ICR; D010853:Picryl Chloride / Q000009:adverse effects / Q000494:pharmacology; D010936:Plant Extracts / Q000494:pharmacology; D013150:Spirostans / Q000302:isolation & purification / Q000494:pharmacology*
D006027:Glycosides; D010936:Plant Extracts; D013150:Spirostans; C012541:ruscogenin; D010853:Picryl Chloride