Biosensors as new analytical tool for detection of Genetically Modified Organisms (GMOs).
Three different biosensors for detection of Genetically Modified Organisms (GMOs) are presented. The sensing principle is based on the affinity interaction between nucleic acids: the probe is immobilised on the sensor surface and the target analyte is free in solution. The immobilised probes are specific for most inserted sequences in GMOs: the promoter P35S and the terminator TNOS. Electrochemical methods with screen-printed electrodes, piezoelectric and optical (SPR) transduction principles were applied.
Minunni
M
M
Università degli Studi di Firenze, Dipartimento di Chimica, Italy. minunni@unifi.it
Tombelli
S
S
Mariotti
E
E
Mascini
M
M
Mascini
M
M
D015374:Biosensing Techniques; D004247:DNA / Q000032:analysis* / Q000235:genetics*; D004251:DNA Transposable Elements; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods; D004566:Electrodes; D005818:Genetic Engineering; D030821:Plants, Genetically Modified; D016133:Polymerase Chain Reaction / Q000379:methods; D011401:Promoter Regions, Genetic; D013728:Terminator Regions, Genetic
D004251:DNA Transposable Elements; D004247:DNA