Cleavage of mitochondria-like transfer RNAs expressed in Escherichia coli.
Mitochondrial (mt) transfer RNAs (tRNAs) often harbor unusual structural features causing their secondary structure to differ from the conventional cloverleaf. tRNAs designed with such irregularities, termed mt-like tRNAs, are active in Escherichia coli as suppressors of reporter genes, although they display low steady-state levels. Characterization of fragments produced during mt-like tRNA processing in vitro and in vivo suggests that these RNAs are not fully processed at their 5' ends and are cleaved internally. These abnormal processing events may account for the low levels of mature mt-like RNAs in vivo and are most likely related to defective processing by RNase P.
Bourdeau
V
V
Département de Biochimie, Université de Montréal, Montréal, QC, Canada. bourdeau@cshl.org
Paquin
B
B
Cedergren
R
R
Delihas
N
N
D020121:5' Untranslated Regions / Q000378:metabolism; D015152:Blotting, Northern; D004926:Escherichia coli / Q000378:metabolism*; D008928:Mitochondria / Q000235:genetics*; D009690:Nucleic Acid Conformation; D016376:Oligonucleotides, Antisense / Q000378:metabolism; D012323:RNA Processing, Post-Transcriptional / Q000502:physiology*; D020871:RNA Stability / Q000502:physiology; D012343:RNA, Transfer / Q000378:metabolism*
D020121:5' Untranslated Regions; D016376:Oligonucleotides, Antisense; D012343:RNA, Transfer