Azithromycin inhibits quorum sensing in Pseudomonas aeruginosa.
We report that 2 microg of azithromycin/ml inhibits the quorum-sensing circuitry of Pseudomonas aeruginosa strain PAO1. Addition of synthetic autoinducers partially restored the expression of the trancriptional activator-encoding genes lasR and rhlR but not that of the autoinducer synthase-encoding gene lasI. We propose that azithromycin interferes with the synthesis of autoinducers, by an unknown mechanism, leading to a reduction of virulence factor production.
Tateda
K
K
Department of Microbiology, Toho University School of Medicine, Tokyo, Japan.
Comte
R
R
Pechere
J C
JC
Köhler
T
T
Yamaguchi
K
K
Van Delden
C
C
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D017963:Azithromycin / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000187:drug effects*; D004268:DNA-Binding Proteins / Q000187:drug effects; D007763:Lac Operon / Q000187:drug effects; D026901:Membrane Transport Proteins; D011550:Pseudomonas aeruginosa / Q000187:drug effects*; D015534:Trans-Activators / Q000187:drug effects
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C080365:LasR protein, Pseudomonas aeruginosa; D026901:Membrane Transport Proteins; C408983:QsrP protein, Vibrio fischeri; D015534:Trans-Activators; C082573:xcpR protein, Pseudomonas aeruginosa; D017963:Azithromycin