[Search for the spirochete Borrelia burgdorferi sensu lato by polymerase chain reaction in wild Chilean ticks].
BACKGROUND
Lyme disease is a tick-borne human disease caused by the spirochete Borrelia burgdorferi. Main vectors of Lyme disease are ticks of the Ixodes and Amblyomma genera. Cases with clinical manifestations of Lyme disease and favorable responses to antimicrobial agents have been reported in Chile, some of them with erythema migrans, the hallmark of B burgdorferi infection.


OBJECTIVE
To detect the presence of B burgdorferi in Chilean ticks.


METHODS
A total of 62 ticks were recollected from wild rodents and cervidae in the Southern region of Chile. Infected and non infected ticks of the species Ixodes ricimus, were used as controls. Insects were homogenised and B burgdorferi was detected using classical and nested polymerase chain reactions.


RESULTS
B burgdorferi was not detected in the studied ticks.


CONCLUSIONS
Although all the elements required for the enzootic cycle of B burgdorferi are present in Chile, its direct detection in Chilean ticks using the nested polymerase chain reaction assay was negative.
Osorio
G
G
Programa de Microbiología y Micología, Facultad de Medicina, Universidad de Chile, Independencia 1027, Casilla 13898-Correo 21, Santiago, Chile. gosorio@canela.med.uchile.cl
D000818:Animals; D001483:Base Sequence; D015748:Borrelia burgdorferi Group / Q000235:genetics / Q000302:isolation & purification*; D016022:Case-Control Studies; D002677:Chile; D003670:Deer / Q000469:parasitology; D018884:Ixodes / Q000382:microbiology*; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012377:Rodentia / Q000469:parasitology