Effect of prostaglandin A1, arsenite and aspirin on stress proteins response in mosquito cells.
The stress response of eukaryotic cells is characterized by changes in the metabolism of responding cells, most notably by increased synthesis of a group of proteins known as heat shock (HSP) proteins In this paper the effect of prostaglandin A1 (PGA1), arsenite and aspirin in Aedes albopictus cells was investigated. In cells treated with PGA1 (10 microg/ml) we observed the induction of several polypeptides with molecular masses of 87, 80, 70, 57, 29 and 23 kDa. Immunoblot analysis revealed that arsenite induces a marked synthesis of HSP70, and aspirin administered during the hyperthermic treatment caused a small increase of HSP70 synthesized.
de Meneses
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MD
Departamento de Virologia, Instituto de Microbiologia Professor Paulo de Goés, Universidade Federal do Rio de Janeiro, Brasil.
Rebello
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MA
D000330:Aedes; D000818:Animals; D018053:Arsenites / Q000494:pharmacology*; D001241:Aspirin / Q000494:pharmacology*; D002460:Cell Line; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis*; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D015151:Immunoblotting; D008715:Methionine / Q000378:metabolism; D008970:Molecular Weight; D011454:Prostaglandins A / Q000494:pharmacology*
D018053:Arsenites; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D011454:Prostaglandins A; D008715:Methionine; C015001:arsenite; D001241:Aspirin; C100573:prostaglandin A1