Synthesis of a disulfide-linked octameric peptide construct carrying three different antigenic determinants.
In an effort to develop peptide vaccines against the influenza virus, we have successfully synthesized a disulfide-linked octameric homodimer that bears four copies of the influenza virus M2 protein ectodomain as well as two copies each of T-helper cell hemagglutinin epitopes, the I-E(d) restricted S1 and the I-A(d) restricted S2 fragments. Peptide attachment was via intermolecular disulfide formation from free sulfhydryl-bearing cysteine derivatives in solution. This reaction was efficient only when the amino-group of the cysteine was Fmoc-protected.
Kragol
G
G
The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA. kragol@wistar.upenn.edu
Otvos
L
L
Feng
J
J
Gerhard
W
W
Wade
J D
JD
D000595:Amino Acid Sequence; D000818:Animals; D003545:Cysteine / Q000737:chemistry / Q000378:metabolism; D004220:Disulfides / Q000737:chemistry*; D000939:Epitopes; D051379:Mice; D017434:Protein Structure, Tertiary; D022223:Vaccines, Subunit / Q000138:chemical synthesis / Q000737:chemistry / Q000276:immunology; D014763:Viral Matrix Proteins / Q000138:chemical synthesis* / Q000737:chemistry / Q000276:immunology; D000086082:Viroporin Proteins
D003545:Cysteine; D004220:Disulfides; D000939:Epitopes; D022223:Vaccines, Subunit; D014763:Viral Matrix Proteins; C031540:M-protein, influenza virus; C488937:M2 protein, Influenza A virus; D000086082:Viroporin Proteins