Crucial importance of PKC-beta(I) in LFA-1-mediated locomotion of activated T cells.
Crawling T cell locomotion in which activated lymphocyte function-associated antigen 1 (LFA-1) integrins participate is associated with translocation of the protein kinase C-beta (PKC-beta) isoenzyme to the microtubule cytoskeleton. In normal T cells and T lymphoma cell lines, this type of motility is accompanied by PKC-beta-sensitive cytoskeletal rearrangements and the formation of trailing cell extensions, which are supported by microtubules. Expression of PKC-beta(I) and enhanced green fluorescent protein (EGFP) in nonmotile PKC-beta-deficient T cells restored their locomotory behavior in response to a triggering stimulus delivered via LFA-1 and correlated directly with the degree of cell polarization. We have also shown that PKC-beta(I) is a component of the tubulin-enriched LFA-1-cytoskeletal complex assembled upon LFA-1 cross-linking. These observations may have physiological equivalents at advanced (post-integrin activation) stages of lymphocyte extravasation.
Volkov
Y
Y
Dublin Molecular Medicine Centre, Trinity College, Dublin 8, Ireland. yvolkov@tcd.ie
Long
A
A
McGrath
S
S
Ni Eidhin
D
D
Kelleher
D
D
D002460:Cell Line; D002465:Cell Movement / Q000502:physiology; D016764:Cell Polarity / Q000276:immunology / Q000502:physiology; D003599:Cytoskeleton / Q000502:physiology; D049452:Green Fluorescent Proteins; D006801:Humans; D066298:In Vitro Techniques; D007527:Isoenzymes / Q000235:genetics / Q000502:physiology*; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008213:Lymphocyte Activation; D016169:Lymphocyte Function-Associated Antigen-1 / Q000502:physiology*; D008870:Microtubules / Q000502:physiology; D011493:Protein Kinase C / Q000235:genetics / Q000502:physiology*; D064546:Protein Kinase C beta; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology* / Q000502:physiology*
D007527:Isoenzymes; D008164:Luminescent Proteins; D016169:Lymphocyte Function-Associated Antigen-1; D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins; D011493:Protein Kinase C; D064546:Protein Kinase C beta