Impact of prednisone on TGF-beta1 and collagen in diaphragm muscle from mdx mice.
The purpose of this study was to assess the impact of prednisone treatment for 8 weeks on the level of transforming growth factor-beta 1 (TGF-beta1), hydroxyproline (HYP) concentrations, and level of the mature, nonreducible collagen cross-link hydroxylysylpyridinoline (HP) in diaphragm muscle from 12-week-old mdx mice. Diaphragm muscle from untreated mdx mice had a significantly higher level of TGF-beta1, HYP, and HP cross-link compared with normal C57BL/10J (control) mice. Prednisone treatment significantly reduced the level of TGF-beta1 and HYP in diaphragm from mdx mice to values similar to control mice, but resulted in a higher level of the HP cross-link compared with untreated mdx mice. These findings indicate that short-term treatment of mdx mice with prednisone can attenuate the fibrotic response in diaphragm muscle, possibly by mediating the level of TGF-beta. Although prednisone was beneficial in preventing collagen accumulation, it resulted in a higher level of the HP cross-link, presumably by decreasing collagen turnover
Hartel
J V
JV
Department of Physical Therapy, Exercise and Nutrition Sciences, 405 Kimball Tower, State University of New York at Buffalo, Buffalo, New York 14214, USA.
Granchelli
J A
JA
Hudecki
M S
MS
Pollina
C M
CM
Gosselin
L E
LE
D000818:Animals; D003094:Collagen / Q000032:analysis*; D003432:Cross-Linking Reagents / Q000032:analysis; D003964:Diaphragm / Q000737:chemistry* / Q000187:drug effects; D005938:Glucocorticoids / Q000494:pharmacology*; D006909:Hydroxyproline / Q000032:analysis; D051379:Mice; D008810:Mice, Inbred C57BL; D018101:Mice, Inbred mdx; D011241:Prednisone / Q000494:pharmacology*; D016212:Transforming Growth Factor beta / Q000032:analysis*; D053773:Transforming Growth Factor beta1
D003432:Cross-Linking Reagents; D005938:Glucocorticoids; C508961:Tgfb1 protein, mouse; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; D003094:Collagen; D006909:Hydroxyproline; D011241:Prednisone