title: Nucleotide sequence analysis of the binding site on the inositol 1,4,5-trisphosphate type-1 receptor in bipolar disorder -- a negative study.
abstract: Pharmacological studies of bipolar disorder suggest that dysfunction of calcium mobilization via phosphatidylinositol-mediated transduction may be involved in its pathogenesis. The present study tests the hypothesis that dysfunction of calcium mobilization in bipolar disorder is due to the mutation of the nucleotide sequence in the FKBP12 binding site on the inositol 1,4,5-trisphosphate type-1 receptor (IP(3)R1). Nucleotide sequence analysis of the FKBP12 binding site on IP(3)R1 was performed using reverse transcription-polymerase chain reaction and DNA sequencing. The nucleotide sequence in this region was preserved in all subjects. This finding suggests that IP(3)R1 dysfunction through the FKBP12 binding site is not involved in the pathogenesis of bipolar disorder.
authors.affiliation: Department of Psychiatry, Shiga University of Medical Science, Otsu, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Fujimaki
authors.forename: S
authors.initials: S
authors.lastname: Morinobu
authors.forename: J
authors.initials: J
authors.lastname: Takahashi
authors.forename: S
authors.initials: S
authors.lastname: Yamawaki
authors.forename: N
authors.initials: N
authors.lastname: Kato
authors.forename: M
authors.initials: M
authors.lastname: Kanno
authors.forename: N
authors.initials: N
authors.lastname: Okuyama
authors.forename: S
authors.initials: S
authors.lastname: Kawakatsu
authors.forename: K
authors.initials: K
authors.lastname: Otani
authors.forename: I
authors.initials: I
authors.lastname: Kusumi
authors.forename: T
authors.initials: T
authors.lastname: Koyama
mesh_terms: D000328:Adult; D001483:Base Sequence; D001714:Bipolar Disorder / Q000235:genetics / Q000503:physiopathology*; D015220:Calcium Channels / Q000235:genetics*; D004252:DNA Mutational Analysis; D005260:Female; D006801:Humans; D015544:Inositol 1,4,5-Trisphosphate / Q000235:genetics* / Q000494:pharmacology; D053496:Inositol 1,4,5-Trisphosphate Receptors; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D022061:Tacrolimus Binding Protein 1A / Q000235:genetics* / Q000494:pharmacology
chemical_list: D015220:Calcium Channels; C506926:ITPR1 protein, human; D053496:Inositol 1,4,5-Trisphosphate Receptors; D018160:Receptors, Cytoplasmic and Nuclear; D015544:Inositol 1,4,5-Trisphosphate; D022061:Tacrolimus Binding Protein 1A