title: Isolation and characterization of a full-length molecular DNA clone of Ghanaian HIV type 1 intersubtype A/G recombinant CRF02_AG, which is replication competent in a restricted host range.
abstract: We have isolated a replication-competent, full-length molecular clone of HIV-1 CRF02_AG, designated p97GH-AG1, by reconstituting two separately amplified genomic regions of an HIV-1 provirus of a 1997 Ghanaian isolate. The phylogenetic and recombination breakpoint analyses revealed that 97GH-AG1 had an A/G recombinant structure similar to that of prototype Nigerian isolate IbNG. The 17-nucleotide insertion downstream of the primer-binding site appeared to be a common sequence signature specific to most CRF02_AG strains, including 97GH-AG1. 97GH-AG1 showed an R5 phenotype and exerted productive infection in both HOS and NP2 cell infectivity assays, whereas it failed to show a detectable level of progeny production in peripheral blood mononuclear cells (PBMCs). The data may suggest the presence of unknown determinant(s) that dictate efficient replication in PBMCs, but that are not required for replication in immortalized cell lines.
authors.affiliation: AIDS Research Center, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Kusagawa
authors.forename: Y
authors.initials: Y
authors.lastname: Takebe
authors.forename: R
authors.initials: R
authors.lastname: Yang
authors.forename: K
authors.initials: K
authors.lastname: Motomura
authors.forename: W
authors.initials: W
authors.lastname: Ampofo
authors.forename: J
authors.initials: J
authors.lastname: Brandful
authors.forename: Y
authors.initials: Y
authors.lastname: Koyanagi
authors.forename: N
authors.initials: N
authors.lastname: Yamamoto
authors.forename: T
authors.initials: T
authors.lastname: Sata
authors.forename: K
authors.initials: K
authors.lastname: Ishikawa
authors.forename: Y
authors.initials: Y
authors.lastname: Nagai
authors.forename: M
authors.initials: M
authors.lastname: Tatsumi
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D004279:DNA, Viral; D005260:Female; D005869:Ghana; D006679:HIV Seropositivity / Q000821:virology*; D015497:HIV-1 / Q000145:classification* / Q000235:genetics*; D006801:Humans; D008875:Middle Aged; D008969:Molecular Sequence Data; D009710:Nucleotide Mapping; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011995:Recombination, Genetic; D014779:Virus Replication
chemical_list: D004279:DNA, Viral