[Comparison of endometrial histology and ultrastructure during different ovarian stimulation protocols in hamsters].
OBJECTIVE
To evaluate the influence of ovarian stimulation by pregnant mare's serum gonadotropin(PMSG) with or without gonadotropin releasing hormone agonist (GnRH-a) on endometrial histology and ultrastructure in hamsters.


METHODS
Thirty hamsters were randomly allocated into 3 groups. (1) LA + PMSG group: Leuprolide acetate (LA) was given first for desensitizing the pituitary, then PMSG added for ovarian stimulation. (2) PMSG group was injected with PMSG only. (3) Control group was given with saline of same volume as groups LA + PMSG and PMSG on the same time schedule. Endometrial histology and ultrastructure were investigated by light microscope and electron microscope.


RESULTS
Comparing to the controls, the changes of endometrial histology in group of LA + PMSG was not significantly different, but its ultrastructure had marked atterations: mitochondria swelling, undifferentiated crista and no basket-like structure. Delayed endometrial maturation was frequently found in group PMSG (P < 0.01), and the ultrastructure showed poorly developed mitochondria.


CONCLUSIONS
The degree of endometrial maturation varied considerably with different ovarian stimulation protocols. Ovarian stimulation with GnRH-a was better than PMSG alone, because the protocol LA + PMSG, in hamsters, facilitated the formation of a physiologic endometrium. But the protocol with GnRH-a still had a negative effect on endometrial ultrastructure.
Huang
H
H
Obstetrics and Gynecology Hospital, Zhejiang University, Hangzhou 310006.
Shi
Y
Y
Zhou
F
F
D000818:Animals; D006224:Cricetinae; D004717:Endometrium / Q000648:ultrastructure*; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000494:pharmacology; D006064:Gonadotropins, Equine / Q000494:pharmacology*; D008647:Mesocricetus; D010062:Ovulation Induction; D011897:Random Allocation; D013480:Superovulation
D006064:Gonadotropins, Equine; D007987:Gonadotropin-Releasing Hormone