Heat shock stimulation of a tilapia heat shock protein 70 promoter is mediated by a distal element.
We reported previously that a tilapia (Oreochromis mossambicus) heat shock protein 70 (HSP70) promoter is able to confer heat shock response on a reporter gene after transient expression both in cell culture and in microinjected zebrafish embryos. Here we present the first functional analysis of a fish HSP70 promoter, the tiHSP70 promoter. Using transient expression experiments in carp EPC (epithelioma papulosum cyprini) cells and in microinjected zebrafish embryos, we show that a distal heat shock response element (HSE1) at approx. -800 is predominantly responsible for the heat shock response of the tiHSP70 promoter. This element specifically binds an inducible transcription factor, most probably heat shock factor, and a constitutive factor. The constitutive complex is not observed with the non-functional, proximal HSE3 sequence, suggesting that both factors are required for the heat shock response mediated by HSE1.
Molina
A
A
Laboratoire de Biologie Moléculaire et Génie Génétique, Université de Liège, Institut de Chimie B6, B-4000 Sart-Tilman, Belgium.
Di Martino
E
E
Martial
J A
JA
Muller
M
M
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D002347:Carps / Q000235:genetics; D002478:Cells, Cultured; D004247:DNA / Q000235:genetics / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics; D015870:Gene Expression; D017930:Genes, Reporter; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*; D006358:Hot Temperature; D016297:Mutagenesis, Site-Directed; D011401:Promoter Regions, Genetic; D017386:Sequence Homology, Amino Acid; D017210:Tilapia / Q000235:genetics*; D014157:Transcription Factors / Q000378:metabolism; D015027:Zebrafish / Q000196:embryology / Q000235:genetics
D017931:DNA Primers; D018840:HSP70 Heat-Shock Proteins; D014157:Transcription Factors; D004247:DNA