Actin is oxidized during myocardial ischemia.
Exposure of isolated rat hearts to 30 min global ischemia followed by 60 min reperfusion resulted in a significant 80% increase (p <.05) in actin content of carbonyl groups, which was associated with significant depression (p <.05) of postischemic contractile function. This result supports the hypothesis that one mechanism of postischemic contractile dysfunction may be oxidation of contractile proteins.
Powell
S R
SR
Maternal/Fetal Medicine Research Lab., Department of Obstetrics and Gynecology, Winthrop University Hospital, Mineola, NY 11501, USA. spowell@winthrop.org
Gurzenda
E M
EM
Wahezi
S E
SE
D000199:Actins / Q000378:metabolism*; D000818:Animals; D001794:Blood Pressure; D006321:Heart / Q000503:physiopathology; D006339:Heart Rate; D015151:Immunoblotting; D066298:In Vitro Techniques; D008297:Male; D009200:Myocardial Contraction; D017202:Myocardial Ischemia / Q000378:metabolism* / Q000503:physiopathology; D015425:Myocardial Reperfusion; D015428:Myocardial Reperfusion Injury / Q000378:metabolism / Q000503:physiopathology; D017682:Myocardial Stunning; D009206:Myocardium / Q000737:chemistry / Q000378:metabolism; D010084:Oxidation-Reduction; D010477:Perfusion; D051381:Rats; D017207:Rats, Sprague-Dawley; D020107:Troponin T / Q000032:analysis / Q000378:metabolism; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000378:metabolism; D016277:Ventricular Function, Left
D000199:Actins; D020107:Troponin T; C002744:3-nitrotyrosine; D014443:Tyrosine