Overlapping CRE and E-box promoter elements can independently regulate COX-2 gene transcription in macrophages.
Macrophage cyclooxygenase-2 (COX-2) transcription is mediated through the collaboration of different promoter elements. Here, the role of an overlapping cyclic AMP responsive element (CRE)/E-box was investigated. Nuclear proteins bound both the CRE and E-box, which synergized with other promoter elements to induce COX-2 transcription. Endotoxin induced binding of nuclear proteins to the CRE and E-box and each element independently induced higher COX-2 transcription levels than the overlapping CRE/E-box. Transcription factors associated with the CRE binding complex included c-Jun and CRE binding protein and with the E-box binding complex USF-1; their overexpression significantly induced COX-2 transcription. Therefore, both CRE and E-box promoter elements regulate COX-2 transcription in macrophages.
Mestre
J R
JR
Rivadeneira
D E
DE
Mackrell
P J
PJ
Duff
M
M
Stapleton
P P
PP
Mack-Strong
V
V
Maddali
S
S
Smyth
G P
GP
Tanabe
T
T
Daly
J M
JM
D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D000242:Cyclic AMP / Q000378:metabolism; D051546:Cyclooxygenase 2; D004731:Endotoxins / Q000378:metabolism / Q000494:pharmacology; D005786:Gene Expression Regulation; D007527:Isoenzymes / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D008957:Models, Genetic; D009154:Mutation; D010957:Plasmids / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism*; D011485:Protein Binding; D016755:Proto-Oncogene Proteins c-jun / Q000378:metabolism; D014158:Transcription, Genetic; D014162:Transfection; D015854:Up-Regulation
D004731:Endotoxins; D007527:Isoenzymes; D016755:Proto-Oncogene Proteins c-jun; D000242:Cyclic AMP; D051546:Cyclooxygenase 2; D011451:Prostaglandin-Endoperoxide Synthases