title: Renal hypertension prevents run training modification of cardiomyocyte diastolic Ca2+ regulation in male rats.
abstract: The combined effects of endurance run training and renal hypertension on cytosolic Ca2+ concentration ([Ca2+]c) dynamics and Na+-dependent Ca2+ regulation in rat left ventricular cardiomyocytes were examined. Male Fischer 344 rats underwent stenosis of the left renal artery [hypertensive (Ht), n = 18] or a sham operation [normotensive (Nt), n = 20]. One-half of the rats from each group were treadmill trained for >16 wk. Cardiomyocyte fura 2 fluorescence ratio transients were recorded for 7 min during electrical pacing at 0.5 Hz, 2 mM extracellular Ca2+ concentration, and 29 degrees C. The rate of [Ca2+]c decline was not changed by run training in the Nt group but was reduced in the Ht group. At 7 min, cardiomyocytes were exposed to 10 mM caffeine in the absence of Na+ and Ca2+, which triggered sarcoplasmic reticular Ca2+ release and suppressed Ca2+ efflux via Na+/Ca2+ exchanger. External Na+ was then added, and Na+-dependent Ca2+ efflux rate was recorded. Treadmill training significantly enhanced Na+-dependent Ca2+ efflux rate under these conditions in the Nt group but not in the Ht group. These data provide evidence that renal hypertension prevents the normal run training-induced modifications in diastolic [Ca2+]c regulation mechanisms, including Na+/Ca2+ exchanger.
authors.affiliation: Department of Kinesiology and Applied Physiology, University of Colorado at Boulder, Boulder, Colorado 80309, USA.
authors.forename: B M
authors.initials: BM
authors.lastname: Palmer
authors.forename: J M
authors.initials: JM
authors.lastname: Lynch
authors.forename: S M
authors.initials: SM
authors.lastname: Snyder
authors.forename: R L
authors.initials: RL
authors.lastname: Moore
mesh_terms: D000818:Animals; D002110:Caffeine / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D002304:Cardiac Pacing, Artificial; D003971:Diastole / Q000502:physiology; D005456:Fluorescent Dyes; D016257:Fura-2; D006321:Heart / Q000502:physiology*; D006977:Hypertension, Renal / Q000503:physiopathology*; D008297:Male; D009206:Myocardium / Q000166:cytology* / Q000378:metabolism; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology; D010805:Physical Conditioning, Animal / Q000502:physiology*; D051381:Rats; D011916:Rats, Inbred F344; D012964:Sodium / Q000378:metabolism
chemical_list: D005456:Fluorescent Dyes; D010726:Phosphodiesterase Inhibitors; D002110:Caffeine; D012964:Sodium; D002118:Calcium; D016257:Fura-2