title: Quantitative trait loci (QTLs) associated with resistance/susceptibility to infectious pancreatic necrosis virus (IPNV) in rainbow trout (Oncorhynchus mykiss).
abstract: Infectious pancreatic necrosis (IPN) is a well-known acute viral disease of salmonid species. We have identified quantitative trait loci (QTLs) associated with resistance to this disease in rainbow trout. We searched for linkage among 51 microsatellite markers used to construct a framework linkage map in backcross families of rainbow trout (Oncorhynchus mykiss), produced by crossing IPN-resistant (YN-RT201) and -susceptible (YK-RT101) strains. Two putative QTLs affecting disease resistance were detected on chromosomes A (IPN R S-1) and C (IPN R/S-2), respectively, suggesting that this is a polygenic trait in rainbow trout. These markers have great potential for use in marker-assisted selection (MAS) for IPN resistance and provide the basis for cloning of IPN resistance genes. Clarification of the genetic bases of complex traits has broad implications for fundamental research, but will also be of practical benefit to fish breeding.
authors.affiliation: Department of Aquatic Biosciences, Tokyo University of Fisheries, Minato, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Ozaki
authors.forename: T
authors.initials: T
authors.lastname: Sakamoto
authors.forename: S
authors.initials: S
authors.lastname: Khoo
authors.forename: K
authors.initials: K
authors.lastname: Nakamura
authors.forename: M R
authors.initials: MR
authors.lastname: Coimbra
authors.forename: T
authors.initials: T
authors.lastname: Akutsu
authors.forename: N
authors.initials: N
authors.lastname: Okamoto
mesh_terms: D000818:Animals; D018175:Birnaviridae Infections / Q000235:genetics / Q000276:immunology / Q000662:veterinary*; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D005393:Fish Diseases / Q000235:genetics* / Q000276:immunology; D005819:Genetic Markers; D007113:Immunity, Innate; D018061:Infectious pancreatic necrosis virus; D018895:Microsatellite Repeats; D017686:Oncorhynchus mykiss / Q000235:genetics* / Q000276:immunology; D019655:Quantitative Trait, Heritable
chemical_list: D005819:Genetic Markers