Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-3-hydroxyhexanoate) by metabolically engineered Escherichia coli strains.
The recombinant Escherichia coli strain, equipped with the newly cloned Aeromonas PHA biosynthesis genes, could produce a terpolymer of 3-hydroxybutyrate (3HB), 3-hydroxyvalerate (3HV), and 3-hydroxyhexanoate (3HHx) [P(3HB-co-3HV-co-3HHx)] from dodecanoic acid plus odd carbon number fatty acid. In addition, the orf1 gene of Aeromonas hydrophila was found to play a critical role in assimilating the 3HV monomer and in regulating the monomer fraction in the terpolymer.
Park
S J
SJ
Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical Engineering and BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology, 373-1 Kusong-dong, Yusong-gu, Taejon 305-701, Korea.
Ahn
W S
WS
Green
P R
PR
Lee
S Y
SY
D000217:Acyltransferases / Q000235:genetics / Q000378:metabolism; D000333:Aeromonas / Q000235:genetics; D004746:Enoyl-CoA Hydratase / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D005285:Fermentation; D005818:Genetic Engineering; D007850:Lauric Acids / Q000378:metabolism; D011108:Polymers / Q000378:metabolism*; D000094122:Polyhydroxybutyrates
D000217:Acyltransferases; D004746:Enoyl-CoA Hydratase; D007850:Lauric Acids; D011108:Polymers; C435024:poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-3-hydroxyhexanoate); D000094122:Polyhydroxybutyrates; C030358:lauric acid; C077225:poly(3-hydroxyalkanoic acid) synthase