[Detection of infection with classical swine fever virus in wild boar: a comparison of different laboratory diagnostic methods].
The aim of this study was to evaluate two commercially available ELISAs for routine diagnosis of classical swine fever virus (CSFV) in wild boar. For this, 222 tissue samples from wild boar were tested in the ELISAs and the results were compared to those obtained using standard methods. First, frozen spleen sections were examined by direct immunofluorescence, and organ suspensions were prepared and tested for CSFV antigen samples were simultaneously examined with the Chekit-ELISA (Dr. Bommeli AG) and the Herd-Chek-ELISA (IDEXX). From the 222 organ suspensions examined in cell culture 102 were positive for CSFV, while no virus could be isolated from the remaining 120 samples. Taking virus isolation as a standard, the Chekit-ELISAs showed a sensitivity of 97%, and the Herd-Chek-ELISA of 72.5%. Both ELISAs revealed high specificities ranging between 99 and 100%. No correlation was found between false negative results obtained in one or in both of the ELISAs with the positive findings in the immunofluorescence test and in the PLA, nor with the clinical reports. Due to the fact that a big number of samples can be processed in a short time with accurate results, the Chekit-ELISA may be considered useful for routine testing of wild boar samples for CSFV.
Hergarten
G
G
Landesuntersuchungsamt Rheinland-Pfalz, Fachbereich Tiermedizin, Institut für Tierseuchendiagnostik, Koblenz.
Hürter
K P
KP
Hess
R G
RG
D000818:Animals; D000835:Animals, Wild; D006691:Classical Swine Fever / Q000175:diagnosis* / Q000276:immunology / Q000821:virology; D006692:Classical Swine Fever Virus / Q000276:immunology / Q000302:isolation & purification*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D019085:Fluorescent Antibody Technique, Direct / Q000662:veterinary; D012680:Sensitivity and Specificity; D013154:Spleen / Q000821:virology; D013552:Swine