title: Expression of c-Fos in Alko alcohol rats responding for ethanol in an operant paradigm.
abstract: BACKGROUND
  Identification of the brain regions involved in ethanol administration is important for understanding the neurobiology of ethanol addiction. Animal studies with different brain mapping techniques found that voluntary ethanol self-administration leads to changes in activity of specific brain regions in patterns that only partially overlap with patterns of brain regions affected by involuntary (i.e., experimenter-administered) ethanol administration. As an extension of studies mapping changes in neural activity after voluntary ethanol drinking, this study analyzed expression of the inducible transcription factor c-Fos after ethanol consumption in an operant procedure.
  
  
  METHODS
  AA (Alko alcohol) rats were trained to operantly respond for water, 0.2% saccharin, 0.2% saccharin/10% (w/v) ethanol, or 10% ethanol in a 30-min limited-access procedure. Animals were allowed to self-administer solutions for at least 40 ethanol response sessions and were killed 1.5 hr after beginning of the last session. Forty-seven brain regions were immunohistochemically analyzed for c-Fos expression.
  
  
  RESULTS
  In this paradigm, ethanol dose-dependently increased c-Fos expression in the Edinger-Westphal nucleus (EW) and decreased expression in the dorsal tenia tecta compared with no-ethanol controls. No effects of saccharin on c-Fos expression were found.
  
  
  CONCLUSIONS
  Our results extend previous findings of preferential sensitivity of EW to alcohol in voluntary self-administration procedures to operant responding for ethanol and warrant further investigation of ethanol's effects on the EW. The finding that ethanol attenuated c-Fos expression in the tenia tecta is novel. Taken together, these findings confirm that voluntary ethanol self-administration leads to changes in activity of a limited number of brain regions with previously unexamined roles in ethanol sensitivity and addiction.
authors.affiliation: Department of Behavioral Neuroscience, Oregon Health Sciences University, Portland, Oregon 97201, USA.
authors.forename: A Z
authors.initials: AZ
authors.lastname: Weitemier
authors.forename: A
authors.initials: A
authors.lastname: Woerner
authors.forename: P
authors.initials: P
authors.lastname: Bäckström
authors.forename: P
authors.initials: P
authors.lastname: Hyytiä
authors.forename: A E
authors.initials: AE
authors.lastname: Ryabinin
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D002492:Central Nervous System Depressants / Q000494:pharmacology*; D003216:Conditioning, Operant / Q000187:drug effects* / Q000502:physiology; D000431:Ethanol / Q000494:pharmacology*; D008297:Male; D016760:Proto-Oncogene Proteins c-fos / Q000187:drug effects* / Q000378:metabolism; D051381:Rats
chemical_list: D002492:Central Nervous System Depressants; D016760:Proto-Oncogene Proteins c-fos; D000431:Ethanol