Genomic organization of the CC chemokine mip-3alpha/CCL20/larc/exodus/SCYA20, showing gene structure, splice variants, and chromosome localization.
We describe the genomic organization of a recently identified CC chemokine, MIP3alpha/CCL20 (HGMW-approved symbol SCYA20). The MIP-3alpha/CCL20 gene was cloned and sequenced, revealing a four exon, three intron structure, and was localized by FISH analysis to 2q35-q36. Two distinct cDNAs were identified, encoding two forms of MIP-3alpha/CCL20, Ala MIP-3alpha/CCL20 and Ser MIP-3alpha/CCL20, that differ by one amino acid at the predicted signal peptide cleavage site. Examination of the sequence around the boundary of intron 1 and exon 2 showed that use of alternative splice acceptor sites could give rise to Ala MIP-3alpha/CCL20 or Ser MIP-3alpha/CCL20. Both forms of MIP-3alpha/CCL20 were chemically synthesized and tested for biological activity. Both flu antigen plus IL-2-activated CD4(+) and CD8(+) T lymphoblasts and cord blood-derived dendritic cells responded to Ser and Ala MIP-3alpha/CCL20. T lymphocytes exposed only to IL-2 responded inconsistently, while no response was detected in naive T lymphocytes, monocytes, or neutrophils. The biological activity of Ser MIP-3alpha/CCL20 and Ala MIP-3alpha/CCL20 and the tissue-specific preference of different splice acceptor sites are not yet known.
Nelson
R T
RT
Chemokine Biology Laboratory, University of Adelaide, Adelaide, South Australia, 5005, Australia.
Boyd
J
J
Gladue
R P
RP
Paradis
T
T
Thomas
R
R
Cunningham
A C
AC
Lira
P
P
Brissette
W H
WH
Hayes
L
L
Hames
L M
LM
Neote
K S
KS
McColl
S R
SR
D017398:Alternative Splicing / Q000235:genetics*; D001483:Base Sequence; D002118:Calcium / Q000378:metabolism; D054418:Chemokine CCL20; D019742:Chemokines, CC / Q000138:chemical synthesis / Q000235:genetics* / Q000502:physiology; D002874:Chromosome Mapping; D002889:Chromosomes, Human, Pair 2; D003001:Cloning, Molecular; D004247:DNA; D005091:Exons; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D007438:Introns; D019402:Macrophage Inflammatory Proteins / Q000138:chemical synthesis / Q000235:genetics* / Q000502:physiology; D008969:Molecular Sequence Data; D054399:Receptors, CCR6; D019707:Receptors, Chemokine; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism
C108355:CCL20 protein, human; C106311:CCR6 protein, human; D054418:Chemokine CCL20; D019742:Chemokines, CC; D019402:Macrophage Inflammatory Proteins; D054399:Receptors, CCR6; D019707:Receptors, Chemokine; D004247:DNA; D002118:Calcium