High-resolution genetic and physical mapping of modifier-of-deafwaddler (mdfw) and Waltzer (Cdh23v).
Modifier-of-deafwaddler (mdfw) and waltzer (Cdh23v) are loci on mouse chromosome 10 encoding factors that are essential for the function of auditory hair cells. The BALB/cByJ-specific mdfw allele encodes a necessary and sufficient modifier that induces progressive early onset hearing loss in CBy-dfw2J heterozygotes. Recessive mutations in the waltzer locus result in circling behavior and congenital deafness. In this report we present a high-resolution integrated genetic and physical map of mdfw and Cdh23(v). Our genetic analyses localize mdfw between markers D10Mit60 and 148M13T7 within a 1.01-cM region. The Cdh23v critical interval is fully contained within the mdfw region and localizes between markers 146O23T7 and 148M13T7 within a 0.35-cM interval that is represented in an approximately 500-kb BAC contig. Our data suggest that mdfw and Cdh23v are allelic.
Bryda
E C
EC
Department of Microbiology, Immunology & Molecular Genetics, Huntington, West Virginia 25704, USA.
Kim
H J
HJ
Legare
M E
ME
Frankel
W N
WN
Noben-Trauth
K
K
D000483:Alleles; D000818:Animals; D002874:Chromosome Mapping; D020451:Contig Mapping; D003638:Deafness / Q000235:genetics*; D005260:Female; D005808:Genes, Recessive / Q000235:genetics; D006239:Haplotypes; D006309:Hearing / Q000235:genetics; D008297:Male; D051379:Mice; D008817:Mice, Mutant Strains; D009154:Mutation / Q000235:genetics*; D010641:Phenotype; D020161:Physical Chromosome Mapping; D011110:Polymorphism, Genetic / Q000235:genetics; D021101:Radiation Hybrid Mapping