title: CDNA for an immune response gene encoding low molecular weight polypeptide from flounder, Paralichthys olivaceus.
abstract: The cDNA for an immune response gene encoding the low molecular weight polypeptide (LMP7) was cloned and sequenced from a flounder (Paralichthys olivaceus) leukocyte cDNA library. The cDNA clone was 1,160 bp, and composed of an open reading frame of 822 bp that corresponded to a protein of 273 amino acid residues with a calculated mass of 30.5 kDa. The ScanProsite search indicated that the deduced amino acid sequence from the flounder LMP7 contains a proteasome beta-type subunit signature, which is well conserved during evolution. The sequence shares a high degree of identity with other LMP7 sequences varying from a 66% identity with zebra fish (Danio renio) to a 57% identity with the African clawed frog (Xenopus laevis), which was confirmed from a phylogenetic tree. A reverse transcription-polymerase chain reaction (RT-PCR) was used to determine tissue specificity, and the expression of LMP7 was detected from the liver, kidney, leukocyte, and spleen of the flounder.
authors.affiliation: Department of Microbiology, Pukyong National University, Pusan, Korea.
authors.forename: J J
authors.initials: JJ
authors.lastname: Cho
authors.forename: B K
authors.initials: BK
authors.lastname: Sung
authors.forename: J H
authors.initials: JH
authors.lastname: Lee
authors.forename: J K
authors.initials: JK
authors.lastname: Chung
authors.forename: T J
authors.initials: TJ
authors.lastname: Choi
authors.forename: Y T
authors.initials: YT
authors.lastname: Kim
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D003001:Cloning, Molecular; D003546:Cysteine Endopeptidases; D018076:DNA, Complementary; D019143:Evolution, Molecular; D005432:Flounder / Q000235:genetics* / Q000276:immunology; D015723:Gene Library; D005802:Genes, MHC Class II; D007962:Leukocytes / Q000502:physiology; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes; D010802:Phylogeny; D046988:Proteasome Endopeptidase Complex; D011506:Proteins / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment
chemical_list: D018076:DNA, Complementary; D009097:Multienzyme Complexes; D011506:Proteins; D003546:Cysteine Endopeptidases; C077436:LMP7 protein; D046988:Proteasome Endopeptidase Complex