title: Neuron-specific expression of Cre recombinase during the late phase of brain development.
abstract: Gene targeting to disrupt gene expression in a temporal and spatial manner in a specific tissue using Cre recombinase-mediated gene inactivation has been proven to be useful to study in vivo gene function. To delete genes specifically in neurons during the late phase of brain development, we have generated transgenic mouse lines that express Cre recombinase under the control of the murine neurofilament-H (mNF-H) gene promoter. In this study, we report that one of these mouse lines expresses Cre recombinase specifically in the neurons of the brain and spinal cord during the late stage of their development. The transgenic line displays specific excision of the loxP-flanked gene in the neurons just after embryonic day 18.5 (E.18.5), which coincides with the later phase of brain maturation including spinal cord and olfactory bulb area. This mNF-H-cre transgenic mouse line will be valuable for studying in vivo functions of neuron-specific genes, particularly, defining their precise roles in the mature nervous system using conditional gene targeting strategies.
authors.affiliation: Functional Genomics Unit, National Institute of Dental and Craniofacial Research, National Institutes of Health, Room 529, Building 30, 30 Convent Drive, Bethesda, MD 20892-4395, USA.
authors.forename: M
authors.initials: M
authors.lastname: Hirasawa
authors.forename: A
authors.initials: A
authors.lastname: Cho
authors.forename: T
authors.initials: T
authors.lastname: Sreenath
authors.forename: B
authors.initials: B
authors.lastname: Sauer
authors.forename: J P
authors.initials: JP
authors.lastname: Julien
authors.forename: A B
authors.initials: AB
authors.lastname: Kulkarni
mesh_terms: D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000196:embryology* / Q000201:enzymology / Q000254:growth & development*; D002454:Cell Differentiation / Q000235:genetics; D005333:Fetus; D005697:Galactosides / Q000493:pharmacokinetics; D017353:Gene Deletion; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D018390:Gene Targeting; D017930:Genes, Reporter / Q000502:physiology; D007211:Indoles / Q000493:pharmacokinetics; D019426:Integrases / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic / Q000196:embryology / Q000235:genetics* / Q000254:growth & development; D016900:Neurofilament Proteins / Q000235:genetics; D009474:Neurons / Q000201:enzymology*; D011401:Promoter Regions, Genetic / Q000502:physiology; D019076:Transgenes / Q000235:genetics; D014764:Viral Proteins
chemical_list: D005697:Galactosides; D007211:Indoles; D016900:Neurofilament Proteins; D014764:Viral Proteins; C051774:neurofilament protein H; C045073:Cre recombinase; D019426:Integrases; C044888:5-bromo-4-chloro-3-indolyl beta-galactoside