Cs(+) induces the kdp operon of Escherichia coli by lowering the intracellular K(+) concentration.
Cs(+) was found to induce expression of the kdpFABC operon, encoding a high-affinity K(+) uptake system of Escherichia coli. Quantitative expression analyses at the transcriptional and translational levels reveal that CsCl causes much higher induction of kdpFABC than does NaCl. A decrease of the intracellular K(+) concentration is found in cells exposed to CsCl. The results indicate that kdpFABC expression is induced when the intracellular K(+) concentration is lowered. Moreover, the results imply that the signal transduction cascade mediated by KdpD and KdpE is able to integrate multiple signals.
Jung
K
K
Abteilung Mikrobiologie, Fachbereich Biologie/Chemie, Universität Osnabrück, D-49069 Osnabrück, Germany. jung_k@biologie.uni-onasbrueck.de
Krabusch
M
M
Altendorf
K
K
D000251:Adenosine Triphosphatases / Q000235:genetics / Q000378:metabolism*; D015152:Blotting, Northern; D015153:Blotting, Western; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D027682:Cation Transport Proteins; D002586:Cesium / Q000378:metabolism* / Q000494:pharmacology; D002712:Chlorides / Q000378:metabolism* / Q000494:pharmacology; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins; D015964:Gene Expression Regulation, Bacterial; D009876:Operon; D011188:Potassium / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D014882:Water-Electrolyte Balance
D002352:Carrier Proteins; D027682:Cation Transport Proteins; D002712:Chlorides; D029968:Escherichia coli Proteins; D012333:RNA, Messenger; D002586:Cesium; D000251:Adenosine Triphosphatases; C058166:potassium translocating Kdp-ATPase, E coli; C028019:cesium chloride; D011188:Potassium