title: A model for a network of phosphorylation-dephosphorylation cycles displaying the dynamics of dominoes and clocks.
abstract: We consider a model for a network of phosphorylation-dephosphorylation cycles coupled through forward and backward regulatory interactions, such that a protein phosphorylated in a given cycle activates the phosphorylation of a protein by a kinase in the next cycle as well as the dephosphorylation of a protein by a phosphatase in a preceding cycle. The network is cyclically organized in such a way that the protein phosphorylated in the last cycle activates the kinase in the first cycle. We study the dynamics of the network in the presence of both forward and backward coupling, in conditions where a threshold exists in each cycle in the amount of protein phosphorylated as a function of the ratio of kinase to phosphatase maximum rates. We show that this system can display sustained (limit-cycle) oscillations in which each cycle in the pathway is successively turned on and off, in a sequence resembling the fall of a series of dominoes. The model thus provides an example of a biochemical system displaying the dynamics of dominoes and clocks (Murray & Kirschner, 1989). It also shows that a continuum of clock waveforms exists of which the fall of dominoes represents a limit. When the cycles in the network are linked through only forward (positive) coupling, bistability is observed, while in the presence of only backward (negative) coupling, the system can display multistability or oscillations, depending on the number of cycles in the network. Inhibition or activation of any kinase or phosphatase in the network immediately stops the oscillations by bringing the system into a stable steady state; oscillations resume when the initial value of the kinase or phosphatase rate is restored. The progression of the system on the limit cycle can thus be temporarily halted as long as an inhibitor is present, much as when a domino is held in place. These results suggest that the eukaryotic cell cycle, governed by a network of phosphorylation-dephosphorylation reactions in which the negative control of cyclin-dependent kinases plays a prominent role, behaves as a limit-cycle oscillator impeded in the presence of inhibitors. We contrast the case where the sequence of domino-like transitions constitutes the clock with the case where the sequence of transitions is passively coupled to a biochemical oscillator operating as an independent clock.
authors.affiliation: Faculté des Sciences, Université Libre de Bruxelles, Campus Plaine, Brussels, B-1050, Belgium.
authors.forename: D
authors.initials: D
authors.lastname: Gonze
authors.forename: A
authors.initials: A
authors.lastname: Goldbeter
mesh_terms: D000818:Animals; D001683:Biological Clocks / Q000502:physiology*; D002453:Cell Cycle / Q000502:physiology*; D000242:Cyclic AMP / Q000502:physiology; D005057:Eukaryotic Cells / Q000166:cytology; D008954:Models, Biological; D010766:Phosphorylation
chemical_list: D000242:Cyclic AMP