Expression of mRNAs for ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), interleukin-6 (IL-6), and their receptors (CNTFR alpha, LIFR beta, IL-6R alpha, and gp130) in human peripheral neuropathies.
The mRNA levels of neuropoietic cytokines, ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and interleukin-6 (IL-6), and their receptor components (CNTFR alpha, LIFR beta, IL-6R alpha, and gp130) were examined in seventy-six patients with various peripheral neuropathies to determine the extent of expression of these cytokines and receptors, and their relationship to nerve fiber pathology and cell infiltration in the diseased nerves. The CNTF mRNA levels were significantly decreased in the diseased nerves and were correlated to residual myelinated fiber population. In contrast, the mRNA levels of LIF, IL-6 and the ligand-binding receptor components (CNTFR alpha, LIFR beta and IL-6R alpha) were elevated to variable extent in the diseased nerves. The CNTFR alpha, LIFR beta, and IL-6R alpha mRNA levels showed a weak positive correlation with the extent of demyelinating pathology and their levels were related to each other. Moreover, the CNTF and LIF mRNA levels were inversely proportional to the extent of macrophage invasion, whereas the CNTFR alpha and IL-6R alpha mRNA expressions were correlated to the increase in macrophage infiltration. The neuropoietic cytokine family and its receptor expressions in the diseased human nerves are regulated by an underlying pathology-related process rather than type of diseases, and could play a role in peripheral nerve regeneration and repair.
Ito
Y
Y
Department of Neurology, Nagoya University School of Medicine, Japan.
Yamamoto
M
M
Mitsuma
N
N
Li
M
M
Hattori
N
N
Sobue
G
G
D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000235:genetics; D020934:Ciliary Neurotrophic Factor / Q000235:genetics*; D050822:Cytokine Receptor gp130; D006131:Growth Inhibitors / Q000235:genetics*; D006801:Humans; D015850:Interleukin-6 / Q000235:genetics*; D053605:Leukemia Inhibitory Factor; D053680:Leukemia Inhibitory Factor Receptor alpha Subunit; D008222:Lymphokines / Q000235:genetics*; D008562:Membrane Glycoproteins / Q000235:genetics; D008875:Middle Aged; D010523:Peripheral Nervous System Diseases / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism*; D020801:Receptor, Ciliary Neurotrophic Factor / Q000235:genetics; D011956:Receptors, Cell Surface / Q000235:genetics*; D018121:Receptors, Cytokine / Q000235:genetics; D019947:Receptors, Interleukin-6 / Q000235:genetics; D053606:Receptors, OSM-LIF; D013497:Sural Nerve / Q000378:metabolism
D015703:Antigens, CD; D020934:Ciliary Neurotrophic Factor; D006131:Growth Inhibitors; C494199:IL6ST protein, human; D015850:Interleukin-6; C507837:LIF protein, human; C507840:LIFR protein, human; D053605:Leukemia Inhibitory Factor; D053680:Leukemia Inhibitory Factor Receptor alpha Subunit; D008222:Lymphokines; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; D020801:Receptor, Ciliary Neurotrophic Factor; D011956:Receptors, Cell Surface; D018121:Receptors, Cytokine; D019947:Receptors, Interleukin-6; D053606:Receptors, OSM-LIF; D050822:Cytokine Receptor gp130