Identification of the self-interaction of rat TCTP/IgE-dependent histamine-releasing factor using yeast two-hybrid system.
To further understand the biological function of translationally controlled tumor protein (TCTP), also known as IgE-dependent histamine-releasing factor (HRF), the yeast two-hybrid system was used to screen interacting molecules. We isolated cDNA clones coding for TCTP/HRF, suggesting that it may have a self-interacting property. Domain mapping of the interaction revealed that the C-terminal region of residue 126-172 is involved in self-interaction. The self-interacting property of TCTP/HRF was further supported by FPLC gel-filtration chromatography and coimmunoprecipitation analysis from transfected COS-7 cells. Our data suggests that TCTP/HRF may have a potential to self-interact through the C-terminal region, and the self-interaction property may be related to its biological function.
Yoon
T
T
College of Pharmacy, Center for Cell Signaling Research, Ewha Woman's University, Seoul, Korea.
Jung
J
J
Kim
M
M
Lee
K M
KM
Choi
E C
EC
Lee
K
K
D000818:Animals; D014408:Biomarkers, Tumor; D019556:COS Cells; D002135:Calcium-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002850:Chromatography, Gel; D008222:Lymphokines / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011233:Precipitin Tests; D017434:Protein Structure, Tertiary; D051381:Rats; D011994:Recombinant Proteins / Q000378:metabolism; D017384:Sequence Deletion; D014162:Transfection; D000090324:Tumor Protein, Translationally-Controlled 1; D020798:Two-Hybrid System Techniques; D015003:Yeasts / Q000235:genetics
D014408:Biomarkers, Tumor; D002135:Calcium-Binding Proteins; D008222:Lymphokines; D011994:Recombinant Proteins; C000715649:Tpt1 protein, rat; D000090324:Tumor Protein, Translationally-Controlled 1