[Comparison of polymerase chain reaction and culture method for assaying ureaplysma urealyticum in urogenital specimens].
OBJECTIVE
A comparison study of polymerase chain reaction (PCR) and culture method were used to detect urogenital ureaplasma urealyticum.


METHODS
Urogenital specimens from 116 patients were assayed in parallel by the two methods.


RESULTS
46 cases were positive by PCR, 42 cases positive by culture, and UuDNA were dedected in 42 cases of the 45 positive by culture. Compared with culture, the sensitivity and specificity of PCR were 93.3% and 94.4%, respectively. The positive rate of both methods were comparable (P > 0.05).


CONCLUSIONS
The PCR technique to detect Uu is highly sensitive and specific. As for culture method, it takes the advantage of being relative simple and takes much less time to give results.
Wang
J
J
PUMC Hospital, CAMS and PUMC, Beijing 100730.
Zheng
Z
Z
Wang
H
H
Liu
Y
Y
Wang
X
X
D000293:Adolescent; D000328:Adult; D003470:Culture Media; D004269:DNA, Bacterial / Q000032:analysis; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D016869:Ureaplasma Infections / Q000382:microbiology*; D014526:Urethritis / Q000382:microbiology*; D002575:Uterine Cervicitis / Q000382:microbiology*
D003470:Culture Media; D004269:DNA, Bacterial