title: Solution structure of the PX domain, a target of the SH3 domain.
abstract: The phox homology (PX) domain is a novel protein module containing a conserved proline-rich motif. We have shown that the PX domain isolated from the human p47phox protein, a soluble subunit of phagocyte NADPH oxidase, binds specifically to the C-terminal SH3 domain derived from the same protein. The solution structure of p47 PX has an alpha + beta structure with a novel folding motif topology and reveals that the proline-rich motif is presented on the molecular surface for easy recognition by the SH3 domain. The proline-rich motif of p47 PX in the free state adopts a distorted left-handed polyproline type II helix conformation.
authors.affiliation: Department of Structural Biology, Biomolecular Engineering Research Institute, 6-2-3, Furuedai, Suita, Osaka 565-0874, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Hiroaki
authors.forename: T
authors.initials: T
authors.lastname: Ago
authors.forename: T
authors.initials: T
authors.lastname: Ito
authors.forename: H
authors.initials: H
authors.lastname: Sumimoto
authors.forename: D
authors.initials: D
authors.lastname: Kohda
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D001665:Binding Sites; D017124:Conserved Sequence; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D019255:NADPH Oxidases / Q000737:chemistry; D019906:Nuclear Magnetic Resonance, Biomolecular; D010750:Phosphoproteins / Q000737:chemistry* / Q000378:metabolism*; D011392:Proline / Q000378:metabolism; D011485:Protein Binding; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D021122:Protein Subunits; D016392:Proto-Oncogene Proteins pp60(c-src) / Q000737:chemistry; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism; D016415:Sequence Alignment; D018909:src Homology Domains
chemical_list: D010750:Phosphoproteins; D021122:Protein Subunits; D011993:Recombinant Fusion Proteins; D011392:Proline; D019255:NADPH Oxidases; C060505:neutrophil cytosolic factor 1; D016392:Proto-Oncogene Proteins pp60(c-src)