[Effects of 3-N-butylphthalide on cortical calcineurin and calpain activities in focal cerebral ischemia rats].
OBJECTIVE
To explore if the inhibitory effect of 3-n-butylphthalide(NBP) on apoptosis induced by transient focal cerebral ischemia in rats is relevant to cortical calcineurin and calpain activities.


METHODS
The model of cerebral ischemia-reperfusion was used. The activities of the two enzymes were measured by using biochemical methods.


RESULTS
DL-NBP and D-NBP 20 mg.kg-1 were found to significantly reduce ischemia ipsilateral cortical calcineurin and calpain activities. However, L-NBP 20 mg.kg-1 showed no obvious effect.


CONCLUSIONS
The anti-apoptotic effect of NBP may be relevant to its inhibition of calcineurin and calpain activities in focal cerebral ischemia rats.
Dong
G X
GX
Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
Feng
Y P
YP
D000818:Animals; D001572:Benzofurans / Q000494:pharmacology*; D002545:Brain Ischemia / Q000378:metabolism*; D019703:Calcineurin / Q000187:drug effects*; D002154:Calpain / Q000187:drug effects*; D002540:Cerebral Cortex / Q000187:drug effects*; D008297:Male; D018696:Neuroprotective Agents / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000378:metabolism
D001572:Benzofurans; D018696:Neuroprotective Agents; C027125:3-n-butylphthalide; D019703:Calcineurin; D002154:Calpain