Preparative separation of the naphthopyranone glycosides by high-speed counter-current chromatography.
High-speed counter-current chromatography was applied to the preparative separation and purification of naphthopyranone glycosides from a crude 70% ethanolic extract of the capitula of Paepalanthus microphyllus. The solvent system used was composed of water-ethanol-ethyl acetate-hexane (10:4:10:4, v/v). This technique led to the separation of four different naphthopyranone glycosides in pure form in only 7 h.
Campaner dos Santos
L
L
Instituto de Química, UNESP, Araraquara, SP, Brazil.
Vilegas
W
W
D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D003377:Countercurrent Distribution / Q000379:methods*; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification*; D019684:Magnoliopsida / Q000737:chemistry*; D009281:Naphthalenes / Q000737:chemistry*; D011753:Pyrones / Q000737:chemistry*
D006027:Glycosides; D009281:Naphthalenes; D011753:Pyrones; C432445:naphthopyrone dimer