title: [Identification and study on effect of new stress protein from Neisseria gonorrhoeae].
abstract: OBJECTIVE
  To investigate the influence of changing environmental growth conditions on the virulence of Neisseria gonorrhoeae.
  
  
  METHODS
  The gonococci were subjected to 1 mmol/L hydrogen peroxide (sublethol concentration) for one hour.
  
  
  RESULTS
  A novel gonococcal stress protein with molecular weight of 62,000 was identified by radioactive labeling and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Gonococci exposed to 1.0 mmol/L hydrogen peroxide made it significantly more resistant to higher concentrations of hydrogen peroxide and to neutrophils than control organisms (P < 0.01).
  
  
  CONCLUSIONS
  These results suggest that the 62,000 stress protein might increase the virulence of N. gonorrhoeae and might be an adapted regular protein produced by N. gonorrhoeae survived within human neutrophils.
authors.affiliation: PUMC Hospital, CAMS and PUMC, Beijing 100730.
authors.forename: H
authors.initials: H
authors.lastname: Zheng
authors.forename: J
authors.initials: J
authors.lastname: Wang
authors.forename: Z
authors.initials: Z
authors.lastname: He
mesh_terms: D018834:Chaperonin 60 / Q000096:biosynthesis*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D009344:Neisseria gonorrhoeae / Q000378:metabolism* / Q000472:pathogenicity; D009504:Neutrophils / Q000502:physiology; D014774:Virulence
chemical_list: D018834:Chaperonin 60; D006861:Hydrogen Peroxide