Expression of growth factors in Dictyostelium discoideum.
Growth factors and their binding proteins are important proteins regulating mammalian cell proliferation and differentiation so there is considerable interest in producing them as recombinant proteins, especially in hosts that do not already produce a complex mixture of growth factors. Many growth factors require post-translational modifications making them unsuitable for production in Escherichia coli or other prokaryotes. Since several expression vector systems have been recently developed for foreign protein production in the cellular slime mould, Dictyostelium discoideum, we attempted to use two of these systems to express human insulin-like growth factor binding protein 6 (hIGFBP6) and bovine beta-cellulin (bBTC) as secreted proteins. Although both proteins were successfully produced in stably transformed amoebae, no secretion was detected in spite of several attempts to facilitate this occurring.
Asgari
S
S
School of Biological Sciences, Flinders University of South Australia, Bedford Park. sassan.asgari@adelaide.edu.au
Arun
S
S
Slade
M B
MB
Marshall
J
J
Williams
K L
KL
Wheldrake
J F
JF
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D066259:Betacellulin; D001709:Biotechnology / Q000379:methods; D002417:Cattle; D004023:Dictyostelium / Q000254:growth & development / Q000378:metabolism*; D004926:Escherichia coli; D005822:Genetic Vectors; D006133:Growth Substances / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D006801:Humans; D018976:Insulin-Like Growth Factor Binding Protein 6 / Q000737:chemistry / Q000235:genetics*; D036341:Intercellular Signaling Peptides and Proteins; D008969:Molecular Sequence Data; D021382:Protein Sorting Signals; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D015183:Restriction Mapping
C587851:BTC protein, human; D066259:Betacellulin; D006133:Growth Substances; D018976:Insulin-Like Growth Factor Binding Protein 6; D036341:Intercellular Signaling Peptides and Proteins; D021382:Protein Sorting Signals; D011994:Recombinant Proteins