Influence of cultivation media on genetic regulatory patterns in Borrelia burgdorferi.
Barbour-Stoenner-Kelly II (BSKII) medium and BSKH medium both are routinely used for the cultivation of Borrelia burgdorferi. However, heretofore there have been no studies to compare how these two media affect gene expression patterns in virulent B. burgdorferi. In the present study, we found that some B. burgdorferi strain 297 genes (e.g., ospA, mlp-7A, mlp-8, p22, and lp6.6) that typically are regulated by temperature or pH displayed their predicted pattern of expression when B. burgdorferi was cultivated in BSKH medium; this was not true when spirochetes were cultivated in conventional BSKII medium. The results suggest that BSKH medium is superior to BSKII medium for gene expression studies with B. burgdorferi.
Yang
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X
Department of Microbiology, University of Texas Southwestern Medical Center, Dallas 75390, USA.
Popova
T G
TG
Goldberg
M S
MS
Norgard
M V
MV
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D015748:Borrelia burgdorferi Group / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism / Q000472:pathogenicity; D003470:Culture Media; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D012710:Serum Albumin, Bovine / Q000494:pharmacology; D013696:Temperature
D001426:Bacterial Proteins; D003470:Culture Media; D012710:Serum Albumin, Bovine