[Changes of prostaglandins in kidney tissue in gentamicin-induced acute renal tubular injury in rats].
OBJECTIVE
To examine the relationship between epidermal growth factor (EGF) and prostaglandins (PGs) in recovery of acute tubular necrosis (ATN) induced by gentamicin in rats and the changes of renal tissue PGs with EGF treatment.


METHODS
Female wistar rats were divided into three groups; normal(NL, n = 7); GM-treated only (Group G, n = 20); GM 200 mg/kg, i.p. x 3 d; GM and EGF-treated (Group G + E, n = 19): EGF(20 micrograms) was given after last GM injection. [3H]thymidine incorporation rate (3HTdR) of renal tissue, serum creatinine concentration (Scr), renal levels of PGE2, 6-keto-PGF1 alpha, TXB2 were measured at day 1,4,8,12 after GM administration.


RESULTS
[3H]thymidine incorporation rate of renal tissue in group G + E was significantly higher than that in group G after toxic injury. The histological lesions of group G + E was less severe than that in group G. 6-keto-PGF1 alpha in group G + E was increased significantly than that in group G, and renal TXB2 in group G + E was lower than that in group G. PGE2 and 6-keto-PGF1 alpha in group G + E was positively correlated with 3HTdR, respectively.


CONCLUSIONS
(1) changes of renal prostaglandins may be related to the injury/proliferation of renal tubular epithelial cells in ATN. (2) Administration of exogenous EGF may enhance the release of PGE2 and 6-keto-PGF1 alpha of renal tissue and inhibit the synthesis of renal TXB2. The results indicate that effect of ameliorating recovery of renal tubular epithelial cells of EGF could be partly related to the changes of renal PGs.
Yu
Y
Y
PUMC Hospital, CAMS and PUMC, Beijing 100730.
Zheng
F
F
Bo
Y
Y
Duan
J
J
Huang
H
H
D015121:6-Ketoprostaglandin F1 alpha / Q000378:metabolism*; D000818:Animals; D015232:Dinoprostone / Q000378:metabolism; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005260:Female; D005839:Gentamicins; D007668:Kidney / Q000378:metabolism*; D007683:Kidney Tubular Necrosis, Acute / Q000139:chemically induced / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013929:Thromboxane B2 / Q000378:metabolism*
D005839:Gentamicins; D013929:Thromboxane B2; D015121:6-Ketoprostaglandin F1 alpha; D004815:Epidermal Growth Factor; D015232:Dinoprostone