Cloning and sequencing of a novel human gene which encodes a putative hydroxylase.
Using a conventional two-hybrid technique with MAWD as bait protein, a novel full-length cDNA was isolated and sequenced from a human liver cDNA library. This cDNA consists of 2,575 base pairs and has a predicted open reading frame encoding 255 amino acids. Overall, it is similar to the catalytic enzyme PHZF, catalyzing the hydroxylation of phenazine-1-carboxylic acid to 2-hydroxyphenazine-1-carboxylic acid. Polymerase chain reaction-based mapping with both a monochromosomal hybrid panel and radiation hybrid cell panels placed the gene to human chromosome 10q21.1 near the marker D10S210.
Iriyama
C
C
Department of Molecular Pathogenesis, Nagoya University School of Medicine, Japan.
Matsuda
S
S
Katsumata
R
R
Hamaguchi
M
M
D001483:Base Sequence; D002879:Chromosomes, Human, Pair 10 / Q000235:genetics; D003001:Cloning, Molecular; D018076:DNA, Complementary; D006801:Humans; D008099:Liver / Q000201:enzymology; D006899:Mixed Function Oxygenases / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000378:metabolism; D009928:Organ Specificity; D010619:Phenazines / Q000378:metabolism; D020161:Physical Chromosome Mapping; D011506:Proteins; D016601:RNA-Binding Proteins; D021101:Radiation Hybrid Mapping; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D020798:Two-Hybrid System Techniques
D018076:DNA, Complementary; D009363:Neoplasm Proteins; C434327:PBLD protein, human; D010619:Phenazines; D011506:Proteins; D016601:RNA-Binding Proteins; C403447:STRAP protein, human; C037165:1-phenazinecarboxylic acid; D006899:Mixed Function Oxygenases