Determination of retinoids in human serum, tocopherol and retinyl acetate in pharmaceuticals by RP-LC with electrochemical detection.
A liquid chromatography with a electrochemical detector method has been developed of the quantitative measurement for the three retinoids in human serum (13-cis and all-trans retinoic acid and retinol), as well as tocopherol acetate, retinyl acetate and retinol in pharmaceuticals. The detection cell consisted of a glassy carbon electrode held at 1.0 V versus an Ag/AgCl reference electrode. The maximum electrochemical signal was obtained with a supporting electrolyte containing 92% methanol 0.1 M acetate buffer (pH 4.72) as the mobile phase. The quantification limits are 0.5, 0.2, 0.4, 0.5, 0.8 and 0.8 ng for tocopherol acetate, all-trans-RA, 13-cis-RA, retinol, retinal and retinyl acetate, respectively. The electrooxidation process is applied for the simultaneous quantitative determination of retinoids in human serum. Comparison with results obtained from HPLC-UV shows agreement.
Wang
L H
LH
Department of Applied Chemistry, China Nan University of Pharmacy and Science, 71710, Tainan, Taiwan. e201466@ms47.hinet.net
Wang
J F
JF
D000328:Adult; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004224:Diterpenes; D004563:Electrochemistry; D005260:Female; D006801:Humans; D008297:Male; D012176:Retinoids / Q000032:analysis* / Q000097:blood*; D000084562:Retinyl Esters; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D014801:Vitamin A / Q000031:analogs & derivatives / Q000737:chemistry*; D014810:Vitamin E / Q000737:chemistry*
D004224:Diterpenes; D012176:Retinoids; D000084562:Retinyl Esters; D014801:Vitamin A; D014810:Vitamin E; C009166:retinol acetate