Interleukin-1beta exacerbates hypoxia-induced neuronal damage, but attenuates toxicity produced by simulated ischaemia and excitotoxicity in rat organotypic hippocampal slice cultures.
Using organotypic hippocampal slice cultures we have investigated the actions of Interleukin-1 (IL-1) in a number of injury paradigms. Low concentrations of IL-1 potentiated hypoxia-induced neurodegeneration whilst high concentrations had no effect. In contrast, higher concentrations of IL-1 were strongly neuroprotective in models of combined oxygen/glucose deprivation and N-methyl-D-aspartate toxicity, but no potentiation was observed at low IL-1 concentrations. Both protective and toxic effects of IL-1 were fully antagonized by IL-1 receptor antagonist. These data demonstrate that the effects of IL-1 on neuronal injury are complex, and may be directly related to the injury paradigm studied.
Pringle
A K
AK
Centre for Neuroscience, University of Southampton, Boldrewood Building, Bassett Crescent East, SO16 7PX, Southampton, UK. akp @soton.ac.uk
Niyadurupola
N
N
Johns
P
P
Anthony
D C
DC
Iannotti
F
F
D000818:Animals; D002545:Brain Ischemia / Q000378:metabolism*; D046508:Culture Techniques; D004305:Dose-Response Relationship, Drug; D005947:Glucose / Q000172:deficiency; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology*; D000860:Hypoxia / Q000150:complications / Q000473:pathology*; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1 / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D016202:N-Methylaspartate / Q000037:antagonists & inhibitors / Q000494:pharmacology; D009410:Nerve Degeneration / Q000209:etiology / Q000473:pathology; D009474:Neurons / Q000473:pathology; D018696:Neuroprotective Agents / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D009498:Neurotoxins / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D009994:Osmolar Concentration; D051381:Rats; D017208:Rats, Wistar; D012795:Sialoglycoproteins / Q000494:pharmacology
D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1; D018696:Neuroprotective Agents; D009498:Neurotoxins; D012795:Sialoglycoproteins; D016202:N-Methylaspartate; D005947:Glucose