Alternative splicing is associated with decreased expression of the redox proto-oncogene thioredoxin-1 in human cancers.
Human thioredoxin-1 (Trx-1) is a small redox protein that is overexpressed in a number of human primary tumors, where it is associated with rapid cell proliferation and inhibited apoptosis. Mutation scanning denaturing high-performance liquid chromatography of Trx-1 mRNA in 58 human tumor cell lines found no evidence for changes in the base sequence of human Trx-1 mRNA. An alternatively spliced form of Trx-1 mRNA lacking exons 2 and 3 was found in 7 of the cell lines but it was not translated. The cell lines having the alternatively spliced Trx-1 mRNA had 73% lower total Trx-1 mRNA than the other cell lines, suggesting that alternative splicing may control the level of Trx-1 mRNA in some cancer cells.
Berggren
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MM
Arizona Cancer Center, University of Arizona, Tucson 85724-5024, USA.
Powis
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D017398:Alternative Splicing; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D003110:Colonic Neoplasms / Q000378:metabolism; D004252:DNA Mutational Analysis / Q000379:methods; D005091:Exons; D005260:Female; D020180:Heteroduplex Analysis; D006801:Humans; D007680:Kidney Neoplasms / Q000378:metabolism; D007938:Leukemia / Q000378:metabolism; D008545:Melanoma / Q000378:metabolism; D009369:Neoplasms / Q000378:metabolism*; D010084:Oxidation-Reduction; D020033:Protein Isoforms; D000090063:Proto-Oncogene Mas; D011519:Proto-Oncogenes / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013696:Temperature; D013879:Thioredoxins / Q000235:genetics* / Q000378:metabolism*; D014407:Tumor Cells, Cultured
C000715470:MAS1 protein, human; D020033:Protein Isoforms; D000090063:Proto-Oncogene Mas; D012333:RNA, Messenger; D013879:Thioredoxins