title: Bone marrow-derived macrophages grown in GM-CSF or M-CSF differ in their ability to produce IL-12 and to induce IFN-gamma production after stimulation with Trypanosoma cruzi antigens.
abstract: Trypanosoma cruzi is the etiological agent of Chagas' disease in man. Control of parasitism at the beginning of experimental infection depends on cytokine-activated macrophages that synthesize nitric oxide (NO). We investigated macrophage populations derived in the presence of M-CSF (M-MØ) or GM-CSF (GM-MØ) regarding their ability to control intracellular parasitism by T. cruzi and to synthesize IL-12 and NO. Both macrophage populations supported intracellular multiplication of the parasite; when activated by IFN-gamma, GM-MØ exerted better control of parasitism. Stimulation of GM-MØ with T. cruzi or Staphylococcus aureus resulted in IL-12 production and higher levels of NO synthesis in comparison with stimulated M-MØ. Mice immunized with parasite-Ag-pulsed GM-MØ but not with pulsed M-MØ had increased IFN-gamma and IL-2 production in lymph nodes. However, when immunization was followed by infection with live parasites, transient elevation of IFN-gamma production was observed in both GM-MØ- and M-MØ-immunized mice, without reduction of blood parasite levels.
authors.affiliation: Departamento de Imunologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, Av. Prof. Lineu Prestes, 1730, Ed. Biomédicas IV, São Paulo, 05508-900, SP, Brazil.
authors.forename: C E
authors.initials: CE
authors.lastname: Tadokoro
authors.forename: I
authors.initials: I
authors.lastname: de Almeida Abrahamsohn
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000276:immunology*; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects / Q000276:immunology* / Q000469:parasitology; D002455:Cell Division; D002478:Cells, Cultured; D014355:Chagas Disease / Q000517:prevention & control; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000276:immunology* / Q000494:pharmacology; D007371:Interferon-gamma / Q000096:biosynthesis*; D018664:Interleukin-12 / Q000096:biosynthesis*; D008198:Lymph Nodes; D016173:Macrophage Colony-Stimulating Factor / Q000276:immunology* / Q000494:pharmacology; D008264:Macrophages / Q000166:cytology / Q000187:drug effects / Q000276:immunology* / Q000469:parasitology; D051379:Mice; D008807:Mice, Inbred BALB C; D009569:Nitric Oxide / Q000096:biosynthesis; D014349:Trypanosoma cruzi / Q000254:growth & development / Q000276:immunology*; D014611:Vaccination
chemical_list: D000953:Antigens, Protozoan; D018664:Interleukin-12; D009569:Nitric Oxide; D016173:Macrophage Colony-Stimulating Factor; D007371:Interferon-gamma; D016178:Granulocyte-Macrophage Colony-Stimulating Factor