BimD/SPO76 is at the interface of cell cycle progression, chromosome morphogenesis, and recombination.
BIMD of Aspergillus nidulans belongs to a highly conserved protein family implicated, in filamentous fungi, in sister-chromatid cohesion and DNA repair. We show here that BIMD is chromosome associated at all stages, except from late prophase through anaphase, during mitosis and meiosis, and is involved in several aspects of both programs. First, bimD(+) function must be executed during S through M. Second, in bimD6 germlings, mitotic nuclear divisions and overall cellular program occur more rapidly than in wild type. Thus, BIMD, an abundant chromosomal protein, is a negative regulator of normal cell cycle progression. Third, bimD6 reduces the level of mitotic interhomolog recombination but does not alter the ratio between crossover and noncrossover outcomes. Moreover, bimD6 is normal for intrachromosomal recombination. Therefore, BIMD is probably not involved in the enzymology of recombinational repair per se. Finally, during meiosis, staining of the Sordaria ortholog Spo76p delineates robust chromosomal axes, whereas BIMD stains all chromatin. SPO76 and bimD are functional homologs with respect to their roles in mitotic chromosome metabolism but not in meiosis. We propose that BIMD exerts its diverse influences on cell cycle progression as well as chromosome morphogenesis and recombination by modulating chromosome structure.
van Heemst
D
D
Institut de Génétique et Microbiologie, UMR 8621, Université Paris Sud, 91405 Orsay Cedex, France.
Kafer
E
E
John
T
T
Heyting
C
C
van Aalderen
M
M
Zickler
D
D
D001233:Aspergillus nidulans / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D002453:Cell Cycle; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D015825:Chromosomes, Fungal; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D016193:G1 Phase; D005800:Genes, Fungal; D006361:Heating; D008540:Meiosis; D008741:Methyl Methanesulfonate / Q000494:pharmacology; D008938:Mitosis; D009024:Morphogenesis; D016296:Mutagenesis; D009153:Mutagens / Q000494:pharmacology; D011995:Recombination, Genetic; D016196:S Phase; D029701:Saccharomyces cerevisiae Proteins; D020037:Sordariales
C083254:BIMD protein, Emericella nidulans; D018797:Cell Cycle Proteins; D005656:Fungal Proteins; D009153:Mutagens; D029701:Saccharomyces cerevisiae Proteins; C120540:Spo76 protein, Neurospora crassa; D008741:Methyl Methanesulfonate