title: Rabbit tissue distribution of 3H-ouabain by digital radioautography.
abstract: 3H-ouabain is useful to evaluate the tissue localization of Na,K-ATPase. In this work we determined the distribution of 3H-ouabain in rabbit tissue by digital radioautography. Using this method, we were able to obtain a comparison of various organs in a relatively short time (6.5 days), while with traditional radioautography, only the kidney was detectable after seven months of film exposure. The kidney has the highest intensity (concentration of 3H-ouabain), followed by the heart, liver, muscle, lung, spleen and finally brain, which was almost undetectable. In kidney the activity was higher in the cortex, while the other tissues displayed a more uniform intensity, suggesting that Na,K-ATPase was evenly distributed.
authors.affiliation: CNR Istituto di Fisiologia Clinica, Pisa, Italy. balzan@nsifc.ifc.pi.cnr.its
authors.forename: S
authors.initials: S
authors.lastname: Balzan
authors.forename: L
authors.initials: L
authors.lastname: Di Luca
authors.forename: L
authors.initials: L
authors.lastname: Taddei
authors.forename: P
authors.initials: P
authors.lastname: Salvador
authors.forename: U
authors.initials: U
authors.lastname: Montali
mesh_terms: D000818:Animals; D001345:Autoradiography / Q000379:methods*; D004791:Enzyme Inhibitors / Q000378:metabolism; D007668:Kidney / Q000201:enzymology / Q000378:metabolism; D008099:Liver / Q000201:enzymology / Q000378:metabolism; D008297:Male; D009132:Muscles / Q000201:enzymology / Q000378:metabolism; D009206:Myocardium / Q000201:enzymology / Q000378:metabolism; D010042:Ouabain / Q000378:metabolism*; D011817:Rabbits; D011869:Radioligand Assay; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D013223:Statistics as Topic; D013997:Time Factors; D014018:Tissue Distribution; D014316:Tritium
chemical_list: D004791:Enzyme Inhibitors; D014316:Tritium; D010042:Ouabain; D000254:Sodium-Potassium-Exchanging ATPase