Isolation and characterization of myosin from the adrenal medulla.
A protein with adenosine triphosphatase activity was isolated from bovine adrenal medulla and subsequently purified by ammonium sulfate precipitation and agarose gel filtration using a discontinuous two-buffer system. Characterization of this protein by polyacrylamide gel electrophoresis in the presence of sodium dodecylsulfate, by assay of the activity of Ca2+, K(+)-EDTA and Mg2+ dependent adenosine triphosphatases by amino acid analysis and by electron microscopy has shown that the adrenal medullary myosin closely resembles those myosins isolated from muscle and other non-muscle cells. The possible roles of myosin in the adrenal medulla are discussed.
Trifaró
J M
JM
Department of Pharmacology and Therapeutics, McGill University, Montreal, H3G 1Y6, Quebec, Canada.
Ulpian
C
C
D000313:Adrenal Medulla / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D000595:Amino Acid Sequence / Q000502:physiology; D000818:Animals; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel; D008854:Microscopy, Electron; D009218:Myosins / Q000737:chemistry / Q000302:isolation & purification* / Q000648:ultrastructure; D012967:Sodium Dodecyl Sulfate
D012967:Sodium Dodecyl Sulfate; D009218:Myosins