Binding of dimeric aminoglycosides to the HIV-1 rev responsive element (RRE) RNA construct.
Through a series of elegant fluorescence measurements, particularly through stopped-flow kinetic measurements, it was recently demonstrated that aminoglycoside antibiotics are able to bind to the HIV-1 Rev responsive element (RRE) RNA construct in more than a 1:1 stoichiometry (Lacourciere, K. A.; Stivers, J. T.; Marino, J. P. Biocheminstry 2000, 39, 5630). Here, we present the binding study results of dimeric neomycin ligands through fluorescence anisotropy studies, to the HIV-1 RRE RNA construct. The dimeric neomycin molecules are observed to be able to bind the HIV-1 RRE RNA construct approximately 17-fold higher when compared to the monomeric neomycin, lending evidence that there are indeed two or more neomycin binding sites within the HIV-1 RRE construct.
Tok
J B
JB
Department of Chemistry, Indiana University-Purdue University Fort Wayne, 46805, USA. tok@york.cuny.edu
Dunn
L J
LJ
Des Jean
R C
RC
D000900:Anti-Bacterial Agents / Q000138:chemical synthesis / Q000494:pharmacology*; D001665:Binding Sites / Q000187:drug effects; D002240:Carbohydrate Sequence; D015497:HIV-1 / Q000187:drug effects* / Q000235:genetics; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009355:Neomycin / Q000031:analogs & derivatives / Q000378:metabolism; D012367:RNA, Viral / Q000096:biosynthesis* / Q000187:drug effects / Q000235:genetics; D020218:Response Elements / Q000187:drug effects* / Q000235:genetics*; D013050:Spectrometry, Fluorescence
D000900:Anti-Bacterial Agents; D012367:RNA, Viral; D009355:Neomycin