Effects of proteasome inhibitor PSI on neoplastic and non-transformed cell lines.
In this study we compared the sensitivity of different human and murine cell lines varying in the stage of transformation to dose- and time-dependent cytostatic and/or cytotoxic effects of PSI (a selective proteasome inhibitor), measured by a standard MTT assay. It was found that intensively proliferating cell lines were more sensitive to very small doses of PSI after 24 h incubation than the slow proliferating ones. Non-transformed cell lines showed no sensitivity to PSI, as there was no difference in cell viability in comparison with the control group even after 72 h incubation.
Pleban
E
E
Department of Histology and Embryology, Medical Academy, Warsaw, Poland.
Bury
M
M
Młynarczuk
I
I
Wójcik
C
C
D000465:Algorithms; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D003546:Cysteine Endopeptidases; D011905:Genes, ras / Q000187:drug effects; D006801:Humans; D051379:Mice; D009097:Multienzyme Complexes / Q000037:antagonists & inhibitors*; D009842:Oligopeptides / Q000494:pharmacology*; D011480:Protease Inhibitors / Q000494:pharmacology*; D046988:Proteasome Endopeptidase Complex; D013778:Tetrazolium Salts; D013844:Thiazoles; D014407:Tumor Cells, Cultured
D009097:Multienzyme Complexes; D009842:Oligopeptides; D011480:Protease Inhibitors; D013778:Tetrazolium Salts; D013844:Thiazoles; C090535:benzyloxycarbonyl-isoleucyl-glutamyl(O-tert-butyl)-alanyl-leucinal; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex; C022616:thiazolyl blue