Production of human papillomavirus type 16 early proteins in Bac-To-Bac Expression System (GibcoBRL).
Human papillomavirus type 16 (HPV16) is a major agent in cervical cancer etiology. Its early proteins are responsible for virus persistence, replication and initiation of neoplastic disease. In the present study we describe a use of baculovirus-insect cell expression system for production and study of HPV16 E2 and E4 proteins. The E2 protein binds specifically to viral DNA and E4 protein shows characteristic cytopathic effects on cells.
Schmidt
M
M
Department of Molecular Virology, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University, Poznań, Poland. mschmidt@amu.edu.pl
Olejnik
A K
AK
Stepień
K
K
Olejnik
A M
AM
Butowska
W
W
Grajek
W
W
Warchoł
J B
JB
Gozdzicka-Józefiak
A
A
D000818:Animals; D001345:Autoradiography; D016367:Baculoviridae / Q000235:genetics*; D002460:Cell Line; D002467:Cell Nucleus / Q000235:genetics; D004268:DNA-Binding Proteins; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007313:Insecta; D009856:Oncogene Proteins, Viral / Q000096:biosynthesis* / Q000235:genetics; D027383:Papillomaviridae / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics
D004268:DNA-Binding Proteins; C054855:E2 protein, Human papillomavirus type 16; D009856:Oncogene Proteins, Viral