title: Evaluation of molecular typing methods for Salmonella enterica serovar Typhimurium DT104 isolated in Germany from healthy pigs.
abstract: The discriminatory power of four different DNA based typing methods was tested for the molecular subtyping of Salmonella Typhimurium phage type DT104 isolates. German DT104 strains (n = 133) originating from slaughter pigs were analysed by plasmid profiling, and 32 of them by pulsed-field gel electrophoresis (PFGE) using the restriction enzymes XbaI, SpeI or BlnI, random amplification of polymorphic DNA (RAPD) using 13 different primers and IS200 typing. A resulting subtyping scheme was obtained which is based on the most discriminatory power of the individual methods i.e. plasmid profiling and PFGE with all three enzymes. The index of discrimination obtained by the subtyping scheme was 0.909 closely approaching the maximum value of one. Although minor differences occurred in the molecular DNA pattern of single DT104 strains, a dominating subtyping pattern was observed confirming other studies which showed, that S. Typhimurium DT104 isolates are highly clonal.
authors.affiliation: Federal Institute for Health Protection of Consumers and Veterinary Medicine, Berlin, Germany.
authors.forename: B
authors.initials: B
authors.lastname: Malorny
authors.forename: A
authors.initials: A
authors.lastname: Schroeter
authors.forename: C
authors.initials: C
authors.lastname: Bunge
authors.forename: B
authors.initials: B
authors.lastname: Hoog
authors.forename: A
authors.initials: A
authors.lastname: Steinbeck
authors.forename: R
authors.initials: R
authors.lastname: Helmuth
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques / Q000379:methods / Q000662:veterinary*; D017931:DNA Primers; D004352:Drug Resistance, Microbial; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods / Q000662:veterinary; D005858:Germany / Q000453:epidemiology; D010957:Plasmids / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods / Q000662:veterinary; D015183:Restriction Mapping / Q000662:veterinary; D012481:Salmonella Infections, Animal / Q000453:epidemiology / Q000382:microbiology*; D012486:Salmonella typhimurium / Q000145:classification* / Q000235:genetics; D012680:Sensitivity and Specificity; D013552:Swine / Q000382:microbiology; D013553:Swine Diseases / Q000453:epidemiology / Q000382:microbiology*
chemical_list: D017931:DNA Primers