title: Multi-task flow system for potentiometric analysis: its application to the determination of vitamin B6 in pharmaceuticals.
abstract: A flow set-up based on the sequential injection analysis concept was designed, aiming at increased automation and robustness of procedures related with potentiometric detection in pharmaceutical control. In this sense, programmable set-up calibration, ion-selective electrode characterization, standard addition techniques and titration procedures could be carried out without any stock solutions conventional handling or modification on the physical structure of the flow system. Evaluation of a flow-through vitamin B6 selective electrode and its application to routine analysis of pharmaceuticals were selected as models to demonstrate the system potentialities. The system ability to generate in-line calibrating solutions was verified by comparing the results with those obtained with solutions prepared by the manual procedure. The vitamin B6 determination in pharmaceutical products was carried out and in-line performed recoveries gave values within 97.4-103.5%. The system ability to perform titrations was ascertained using the precipitation reaction of vitamin B6 with tetraphenylborate. Other profitable features such as lower reagent consumption with a low effluent generation volume were also achieved.
authors.affiliation: Centro de Energia Nuclear na Agricultura, Av. Centenário 303, Caixa Postal 96, CEP 13400-970, Piracicaba, Sao Paulo, Brazil.
authors.forename: R N
authors.initials: RN
authors.lastname: Fernandes
authors.forename: M G
authors.initials: MG
authors.lastname: Sales
authors.forename: B F
authors.initials: BF
authors.lastname: Reis
authors.forename: E A
authors.initials: EA
authors.lastname: Zagatto
authors.forename: A N
authors.initials: AN
authors.lastname: Araújo
authors.forename: M C
authors.initials: MC
authors.lastname: Montenegro
mesh_terms: D002138:Calibration; D004566:Electrodes; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D011199:Potentiometry; D011736:Pyridoxine / Q000032:analysis*
chemical_list: D004364:Pharmaceutical Preparations; D011736:Pyridoxine