[Immune response induced by hepatitis B DNA vaccine pCI-S-IRES- ProT alpha].
OBJECTIVE
To investigate immune response in mice inoculated with DNA vaccine.


METHODS
pCI-S and pCI-S-IRES- ProT alpha were constructed by gene technology, transferred into cell line NIH-3T3 mediated by lipofectamine. Mice were inoculated with these plasmids too. HBsAg and HBsAb were detected by ELISA, and transcriptions of these plasmids were detected by RT-PCR.


RESULTS
pCI-S -IRES- ProT alpha, and pCI-S were effectively expressed in cultured cells and vaccinated animals. Humoral immune and specific T-cell proliferative responses were stronger in pCI-S-IRES group than pCI-S group.


CONCLUSIONS
Plasmids coexpression of prothymosin alpha and hepatitis B surface antigen can improve immune responses significantly.
Wang
B
B
Department of Infectious Diseases, Ruijin Hospital, Shanghai 200025, China.
Zhou
X
X
Zhu
J
J
Xie
Q
Q
Zang
G
G
Yu
H
H
Guo
Q
Q
D000818:Animals; D006514:Hepatitis B Surface Antigens / Q000032:analysis; D017325:Hepatitis B Vaccines / Q000276:immunology*; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019444:Vaccines, DNA / Q000276:immunology*
D006514:Hepatitis B Surface Antigens; D017325:Hepatitis B Vaccines; D019444:Vaccines, DNA