[Application of MVR-PCR at D1S8 locus with HRP-labeled MS32 oligonucleotides probes].
The purpose of this study is to detect small amount of biological materials using the method of MVR-PCR at D1S8 locus with HRP-labeled MS32 oligonucleotides probe. The results showed that the MVR-PCR patterns, obtained from trace blood stain (equal to 5 ul blood), trace saliva stain (equal to 4 ul saliva) or a hair root, can be read clearly and easily. The sensitivity of the method is 1 ng DNA. The applications of the method in personal identification were discussed.
Zheng
X
X
Institute of Forensic Science, P.R. China, Beijing 100038.
Ye
J
J
Ni
J
J
Li
B
B
D001801:Blood Stains; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D005260:Female; D005554:Forensic Medicine; D006197:Hair / Q000737:chemistry; D006735:Horseradish Peroxidase; D006801:Humans; D018598:Minisatellite Repeats; D016133:Polymerase Chain Reaction; D012463:Saliva / Q000737:chemistry
D004247:DNA; D006735:Horseradish Peroxidase