Light-induced photoactivation of hypericin inhibits cellular growth in pituitary adenoma cell line AtT20/D16v-F2 (hypericin inhibits cellular growth of AtT20/D16v-F2).
Cultivation with 4-8 mumol/l hypericin (specific proteinkinase C inhibitor) activated by light induced high inhibition of the rate of HL-60 cell growth. When hypericin treated cells were not exposed to light growth inhibition was insignificant. Cultivation with light activated hypericin in concentration 16 mumol/l slightly inhibited growth rate of AtT20/D16v-F2 cells. AtT20/D16v-F2 cells did not proliferate in presence of light activated 32 mumol/l hypericin. Evidence of apoptosis was found in HL-60 cells treated with 1-8 mumol/l light activated hypericin, with maximum of apoptotic cells detected after 8 mumol/l light activated hypericin. Light activated hypericin induces both apoptosis and necrosis in dose and time dependent manners in HL-60 cells, but causes only necrosis in AtT20/D16v-F2 cells.
Mareková
M
M
Department of Medical Biochemistry, Charles University, Faculty of Medicine in Hradec Králové, Prague. marekova@lfhk.cuni.cz
Vávrová
J
J
Vokurková
D
D
Cerman
J
J
D000236:Adenoma / Q000473:pathology*; D000818:Animals; D000873:Anthracenes; D017209:Apoptosis / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D004273:DNA, Neoplasm / Q000032:analysis / Q000187:drug effects; D004307:Dose-Response Relationship, Radiation; D018922:HL-60 Cells / Q000187:drug effects / Q000473:pathology; D006801:Humans; D008027:Light; D051379:Mice; D010569:Perylene / Q000031:analogs & derivatives* / Q000494:pharmacology* / Q000528:radiation effects; D017319:Photosensitizing Agents / Q000494:pharmacology*; D010911:Pituitary Neoplasms / Q000473:pathology*; D011493:Protein Kinase C / Q000037:antagonists & inhibitors*; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000473:pathology
D000873:Anthracenes; D004273:DNA, Neoplasm; D017319:Photosensitizing Agents; D010569:Perylene; C004965:hypericin; D011493:Protein Kinase C