title: Prevalence of genotypes 1 and 2 of bovine viral diarrhea virus in Lower Saxony, Germany.
abstract: The aim of this study was to find whether an antigenic drift had occurred in Lower Saxony in the past 40 years. For this, the genetic diversity of bovine viral diarrhea virus (BVDV) isolates mainly from Lower Saxony was estimated by RT-PCR and sequencing of a 420 bp fragment of the E2 glycoprotein gene. Sixty-one field virus isolates collected during routine diagnostics between 1960 and 2000 in Lower Saxony, Northern Germany, were analyzed. Phylogenetic analysis allowed discrimination of genotypes BVDV 1 and 2. Excepting two isolates, which were of BVDV type 2, most of the isolates were classified as BVDV type 1. This group could be further subdivided into four subgroups and one disparate isolate. Independent of the year of isolation and geographical localization, 54 isolates clustered in two subtypes (BVDV subtypes 1b and 1d). Only one isolate was classified as BVDV type 1a, thus being similar to the North American NADL strain, and to the vaccine strain Oregon C24V, which was extensively used for vaccination in Germany. The remaining isolates belonged to new clusters tentatively designated as BVDV subtypes 1g and 1f. To compare the cluster designation with that of other studies, phylogenetic analysis of representatives of each of the subgroups based on the 5' untranslated region (5'UTR) was performed. It grouped the viruses similarly. The results indicate that the BVDV population seems to be relatively stable over 40 years in Lower Saxony.
authors.affiliation: Department of Clinical Sciences, School of Veterinary Medicine, Hokkaido University, 060-0818, Sapporo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Tajima
authors.forename: H R
authors.initials: HR
authors.lastname: Frey
authors.forename: O
authors.initials: O
authors.lastname: Yamato
authors.forename: Y
authors.initials: Y
authors.lastname: Maede
authors.forename: V
authors.initials: V
authors.lastname: Moennig
authors.forename: H
authors.initials: H
authors.lastname: Scholz
authors.forename: I
authors.initials: I
authors.lastname: Greiser-Wilke
mesh_terms: D020121:5' Untranslated Regions / Q000235:genetics; D000818:Animals; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000453:epidemiology / Q000821:virology*; D002417:Cattle; D030241:Diarrhea Virus 1, Bovine Viral / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D030242:Diarrhea Virus 2, Bovine Viral / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D005787:Gene Frequency / Q000235:genetics; D005838:Genotype; D005858:Germany; D010802:Phylogeny; D015995:Prevalence; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014759:Viral Envelope Proteins / Q000235:genetics
chemical_list: D020121:5' Untranslated Regions; D014759:Viral Envelope Proteins