[Evaluation of immunochromatography method for rapid detection of Shigatoxin].
The Shigatoxin detection kit based on the immunochromatography system is commercially available. To obtain the identification result rapidly, we devised the improved method (ICG-Imp) replaced to an original method (ICG). Modification provided that Shigatoxins extracted directly from the strains grown on TSI medium without centrifugation. ICG-Imp was compared with ICG, RPLA and PCR. Comparing with RPLA, the sensitivity, specificity, and concordance rate of Shigatoxin 1 showed 77.5, 100 and 90.5%, respectively on ICG, 93.8, 100 and 97.4%, respectively on ICG-Imp, and 100, 99.1 and 99.5%, respectively on PCR. On the other hand, the patterns of Shigatoxin 2 showed 95.3, 100 and 96.3%, respectively on ICG, 100, 100, and 100%, respectively on ICG-Imp, and 100, 100 and 100%, respectively on PCR. The time required from TSI medium to the final result are 24 h, 30-60 min, 48 h, and 6 h, respectively by ICG, ICG-Imp, RPLA, and PCR. It seems that the ICG-Imp is recommended for the identification by means of the accuracy and rapidness.
Kurokawa
M
M
Department of Bacteriology, Kobe Institute of Health.
Miyata
T
T
Murase
M
M
Nakanishi
H
H
D002498:Centrifugation; D002845:Chromatography / Q000379:methods*; D004926:Escherichia coli / Q000378:metabolism; D019453:Escherichia coli O157 / Q000378:metabolism; D006801:Humans; D011933:Reagent Kits, Diagnostic / Q000592:standards; D012680:Sensitivity and Specificity; D022621:Shiga Toxin / Q000302:isolation & purification*
D011933:Reagent Kits, Diagnostic; D022621:Shiga Toxin