title: CD1d-reactive T-cell activation leads to amelioration of disease caused by diabetogenic encephalomyocarditis virus.
abstract: A subset of CD161 (NK1) T cells express an invariant Valpha14Jalpha281 TCR-alpha chain (Valpha(invt) T cells) and produce Th2 and Th1 cytokines rapidly in response to CD1d, but their physiological function(s) remain unclear. We have found that CD1d-reactive T cells mediate to resistance against the acute, cytopathic virus diabetogenic encephalomyocarditis virus (EMCV-D) in relatively Th1-biased, C57BL/6-based backgrounds. We show now that these results generalize to Th2-biased, hypersensitive BALB/c mice. CD1d-KO BALB/c mice were more susceptible to EMCV-D. Furthermore, alpha-galactosylceramide (alpha-GalCer), a CD1d-presented lipid antigen that specifically activates Valpha(invt) T cells, protected wild-type (WT) mice against EMCV-D-induced encephalitis, myocarditis, and diabetes. In contrast, neither CD1d-KO nor Jalpha281-KO mice were protected by alpha-GALCER: Finally, disease in Jalpha281-KO mice was comparable to WT, indicating for the first time equivalent roles for CD1d-reactive Valpha(invt) and noninvariant T cells in resistance to acute viral infection. A model for how CD1d-reactive T cells can initiate immune responses, which synthesizes current results, is presented.
authors.affiliation: Cancer Biology Program, Hematology/Oncology Division, Beth Israel-Deaconess Medical Center, and Harvard Medical School, Boston, Massachusetts, USA. mexley@caregroup.harvard.edu
authors.forename: M A
authors.initials: MA
authors.lastname: Exley
authors.forename: N J
authors.initials: NJ
authors.lastname: Bigley
authors.forename: O
authors.initials: O
authors.lastname: Cheng
authors.forename: S M
authors.initials: SM
authors.lastname: Tahir
authors.forename: S T
authors.initials: ST
authors.lastname: Smiley
authors.forename: Q L
authors.initials: QL
authors.lastname: Carter
authors.forename: H F
authors.initials: HF
authors.lastname: Stills
authors.forename: M J
authors.initials: MJ
authors.lastname: Grusby
authors.forename: Y
authors.initials: Y
authors.lastname: Koezuka
authors.forename: M
authors.initials: M
authors.lastname: Taniguchi
authors.forename: S P
authors.initials: SP
authors.lastname: Balk
mesh_terms: D000818:Animals; D018949:Antigens, CD1 / Q000235:genetics / Q000276:immunology*; D055610:Antigens, CD1d; D018188:Cardiovirus Infections / Q000276:immunology*; D004686:Encephalomyocarditis virus / Q000276:immunology*; D005260:Female; D005699:Galactosylceramides / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009205:Myocarditis / Q000276:immunology; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D018418:Th2 Cells / Q000276:immunology
chemical_list: D018949:Antigens, CD1; D055610:Antigens, CD1d; D005699:Galactosylceramides; D016693:Receptors, Antigen, T-Cell, alpha-beta