A comparison of the solution structures of tobacco rattle and tobacco mosaic viruses from Raman optical activity.
Vibrational Raman optical activity (ROA) spectra of tobacco rattle virus (TRV) and tobacco mosaic virus (TMV) were measured and compared with a view to obtaining new information about the coat protein subunit structure of TRV. A sharp strong positive band observed at approximately 1344 cm(-1) in the ROA spectra of the two viruses is evidence that both contain a significant amount of a hydrated form of alpha-helix, but more in TRV than in TMV. Although the ROA spectrum of TMV shows significant positive intensity in the range approximately 1297-1312 cm(-1) characteristic of alpha-helix in a hydrophobic environment, as expected from the helix interface residues in the four-helix bundles that constitute the basic motif of the TMV coat protein fold, that of TRV shows little positive ROA intensity here. Instead TRV shows a strong positive ROA band at approximately 1315 cm(-1), of much greater intensity than bands shown here by TMV, that is characteristic of polyproline II (PPII) helix. This suggests that the additional long central and C-terminal sequences of the TRV coat proteins contain a significant amount of PPII structure, plus perhaps some beta-strand judging by a prominent sharp negative ROA band shown by TRV at approximately 1236 cm(-1), but little alpha-helix. The open flexible hydrated nature of PPII helical structure is consistent with the earlier suggestions that the additional sequences are exposed and, together with a larger amount of hydrated alpha-helix, could serve to fill the extra volume required by the larger diameter of the cylindrical TRV particles relative to those of TMV.
Blanch
Ewan W
EW
Department of Chemistry, University of Glasgow, Glasgow G12 8QQ, UK1.
Robinson
David J
DJ
Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, UK2.
Hecht
Lutz
L
Department of Chemistry, University of Glasgow, Glasgow G12 8QQ, UK1.
Barron
Laurence D
LD
Department of Chemistry, University of Glasgow, Glasgow G12 8QQ, UK1.
D002213:Capsid / Q000737:chemistry* / Q000378:metabolism; D036022:Capsid Proteins; D018583:Pliability; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D021122:Protein Subunits; D012328:RNA Viruses / Q000737:chemistry*; D012996:Solutions; D013059:Spectrum Analysis, Raman; D014027:Tobacco Mosaic Virus / Q000737:chemistry*; D014732:Vibration; D014764:Viral Proteins / Q000737:chemistry* / Q000378:metabolism
D036022:Capsid Proteins; C009496:H protein, Tobacco mosaic virus; D021122:Protein Subunits; D012996:Solutions; D014764:Viral Proteins; C085220:coat protein, Tobacco rattle virus