Angiotensin stimulates the expression of interferon-inducible genes in H295R cells.
Angiotensin-II (A-II) induces proliferation of zona glomerulosa cells and stimulates expression of cytochrome P-450 aldosterone synthase. The genes activated during this adrenal remodeling are not well defined. To clarify this mechanism, we sought to identify the genes whose expression is stimulated by A-II in the H295R cell line. Using a subtractive hybridization technique, we identified one clone whose expression was stimulated by A-II. The sequence of this gene was homologous to the human interferon-inducible genes, 9-27, 1-8D and 1-8U. The 5' portion of the gene was identical to the 1-8D gene product and the 3' was identical to the 9-27 gene product, but the existence of a transcript was not demonstrated by RT-PCR. The expression of these three genes was stimulated by A-II, with the 9-27 gene being most abundant. Potassium and forskolin also stimulated the expression of the 9-27 gene in the H295R cells, but not as effectively as did A-II or interferon-gamma.
Daido
H
H
Division of Endocrinology, University of Missouri, Columbia, MO 65212, USA.
Zhou
M Y
MY
Gomez-Sanchez
C E
CE
D000302:Adrenal Cortex / Q000187:drug effects* / Q000378:metabolism; D000804:Angiotensin II / Q000494:pharmacology*; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D005576:Colforsin / Q000494:pharmacology; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D008969:Molecular Sequence Data; D023282:Nuclease Protection Assays; D009693:Nucleic Acid Hybridization; D011188:Potassium / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015533:Transcriptional Activation / Q000187:drug effects*; D014407:Tumor Cells, Cultured
D012333:RNA, Messenger; D000804:Angiotensin II; D005576:Colforsin; D007371:Interferon-gamma; D011188:Potassium