HIV Rev peptides conjugated with peptide nucleic acids and their efficient binding to RRE RNA.
HIV Rev peptides conjugated with peptide nucleic acids (PNAs) were designed and synthesized to develop a designing approach for a novel RNA-binding molecule. The binding affinities of PNA-peptides with the Rev responsive element (RRE) RNA were determined by the competition assay using a rhodamine-labeled Rev. The peptide conjugated with an antisense PNA (TGCGC) bound RRE RNA more efficiently than the molecule without the PNA or the peptide sequence.
Kumagai
I
I
Department of Bioengineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta, Yokohama, Japan.
Takahashi
T
T
Hamasaki
K
K
Ueno
A
A
Mihara
H
H
D000595:Amino Acid Sequence; D016880:Anisotropy; D002942:Circular Dichroism; D015497:HIV-1 / Q000737:chemistry*; D008969:Molecular Sequence Data; D020135:Peptide Nucleic Acids / Q000737:chemistry*; D012367:RNA, Viral / Q000737:chemistry* / Q000378:metabolism; D020218:Response Elements / Q000187:drug effects*; D013050:Spectrometry, Fluorescence
D020135:Peptide Nucleic Acids; D012367:RNA, Viral