Stem cell factor and interleukin-3 induce stepwise generation of erythroid precursor cells from a basic fibroblast growth factor-dependent hematopoietic stem cell line, A-6.
A multipotent immature myeloid cell population was produced from a basic fibroblast growth factor (bFGF)-dependent hematopoietic stem cell line, A-6, when cultured with stem cell factor (SCF) replacing bFGF. Those cells were positive for stem cell markers, c-kit and CD34, and a myeloid cell marker, F4/80. Some cell fractions were also positive for Mac-1, a macrophage marker or Gr-1, a granulocytic maker, but negative for an erythroid marker TER119. They also showed the expression of mRNA for the myeloid-specific PU.1 but did not that for the erythroid-specific GATA-1. Among various cytokines, interleukin-3 (IL-3) induced erythroid precursor cells that expressed the erythroid-specific GATA-1 and beta-major globin. The quantitative analysis showed that erythroid precursor cells were newly produced from the immature myeloid cells by cultivation with IL-3. SCF and IL-3 induced stepwise generation of erythroid precursor cells from an A-6 hematopoietic stem cell line.
Anzai
H
H
Laboratory of Molecular Cell Science, RIKEN Tsukuba Institute, 3-1-1, Koyadai, Tsukuba, Ibaraki, 305-0074, Japan.
Ikawa
Y
Y
Atsumi
T
T
D000818:Animals; D000943:Antigens, Differentiation / Q000032:analysis; D002454:Cell Differentiation; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics; D015672:Erythroid Precursor Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D050981:Erythroid-Specific DNA-Binding Factors; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D050982:GATA1 Transcription Factor; D005914:Globins / Q000096:biosynthesis / Q000235:genetics; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D016130:Immunophenotyping; D007377:Interleukin-3 / Q000494:pharmacology*; D051379:Mice; D023461:Myeloid Progenitor Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis; D019089:Stem Cell Factor / Q000494:pharmacology*; D015534:Trans-Activators / Q000096:biosynthesis / Q000235:genetics; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics; D000099232:Proto-Oncogene Protein Spi-1
D000943:Antigens, Differentiation; D004268:DNA-Binding Proteins; D050981:Erythroid-Specific DNA-Binding Factors; D016222:Fibroblast Growth Factor 2; D050982:GATA1 Transcription Factor; D005914:Globins; D007377:Interleukin-3; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D019089:Stem Cell Factor; D015534:Trans-Activators; D014157:Transcription Factors; D000099232:Proto-Oncogene Protein Spi-1; C494689:Gata1 protein, mouse; C036763:monocyte-macrophage differentiation antigen