[Effects of glutamic acid and acetylcholine on induction of heat shock proteins 70 mRNA in PC12 cells].
The heat shock response has been found in many strains of bacteria to human beings. Besides heat stimuli, many kinds of factors could also induce the synthesis of heat shock proteins (hsp). It is still unknown whether neurotransmitter could induce the increase of hsp expression in mammalian cells. In the present study, the effects of glutamic acid and acetylcholine (ACh) on the induction of hsp70 mRNA in PC12 cells were studied by Northern blot method. The probe used is specific for inducible hsp70 mRNA. Our results showed that the glutamic acid under limited conditions (such as at 50-500 mumol/L and action time 5-30 min) could induce the expression of hsp70 mRNA, which was partly mediated by NMDA receptors. On the other hand, ACh (0.1-1,000 mumol/L) could not induce the expression of hsp 70 mRNA.
Wu
B Y
BY
Department of Physiology, Second Military Medical University, Shanghai 200433.
Lou
S J
SJ
Xu
R B
RB
Chen
Y Z
YZ
D000109:Acetylcholine / Q000494:pharmacology*; D000818:Animals; D018698:Glutamic Acid / Q000494:pharmacology*; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics; D016716:PC12 Cells / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats
D018840:HSP70 Heat-Shock Proteins; D012333:RNA, Messenger; D018698:Glutamic Acid; D000109:Acetylcholine