Identification of Brucella abortus, B. canis, B. melitensis, and B. suis by carbon substrate assimilation tests.
By using the results of seven carbon substrate assimilation tests from the Biotype 100 system (bioMérieux, Marcy-l'Etoile, France), we correctly identified 79 (85.9%) of 92 Brucella strains tested. The specificity of the method varied from 97.4 to 100% depending on the species. Although a biological safety cabinet must be used, this method represents an easy and fast alternative for the identification of Brucella species.
López-Merino
A
A
Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, IPN, D.F., México, Mexico.
Monnet
D L
DL
Hernández
I
I
Sánchez
N L
NL
Boeufgras
J M
JM
Sandoval
H
H
Freney
J
J
D015373:Bacterial Typing Techniques / Q000379:methods / Q000662:veterinary*; D002002:Brucella / Q000302:isolation & purification*; D002003:Brucella abortus / Q000302:isolation & purification; D017347:Brucella melitensis / Q000302:isolation & purification; D002244:Carbon / Q000378:metabolism
D002244:Carbon