Expression of chloramphenicol acetyltransferase in Bacillus subtilis under the control of a phytoplasma promoter.
A cloned putative promoter region upstream of the 16S rRNA gene of the western X-disease phytoplasma was inserted behind the promoterless chloramphenicol acetyltransferase gene of plasmid pPL603. The DNA construct was used to transform Bacillus subtilis cells. The transformants were assayed for chloramphenicol acetyltransferase activity, showing that the phytoplasma promoter is efficiently expressed in a B. subtilis background.
Palmano
S
S
Dipartimento di Biologia Applicata alla Difesa delle Piante, Università di Udine, Italy. sabrina.palmano@pldef.uniud.it
Kirkpatrick
B C
BC
Firrao
G
G
D000129:Acholeplasmataceae / Q000235:genetics*; D001412:Bacillus subtilis / Q000201:enzymology / Q000235:genetics*; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D017930:Genes, Reporter; D005822:Genetic Vectors / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D011995:Recombination, Genetic; D014169:Transformation, Bacterial
D012336:RNA, Ribosomal, 16S; D015500:Chloramphenicol O-Acetyltransferase