Effect of ionophores on in vitro excystment of Paragonimus ohirai metacercariae.
Ca2+ ionophores (A23187 and ionomycin) induced in vitro excystment of Paragonimus ohirai metacercariae, whereas Na+ ionophore (monensin) and K+ ionophores (nigericin and valinomycin) did not. The effect of A23187 on in vitro excystment was dependent on the concentration of the ionophore. A high concentration of 1 microM or more induced rapid excystment, but resulted in the death of metacercarial juveniles. The appropriate concentration of 0.2 microM induced in vitro excystment by 5 h after incubation with little or no damage to the juveniles.
Ikeda
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Department of Medical Zoology, Kanazawa Medical University, Uchinada, Ishikawa, Japan. ikeda@kanazawa-med.ac.jp
D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D015759:Ionomycin / Q000494:pharmacology; D007476:Ionophores / Q000494:pharmacology*; D010238:Paragonimus / Q000187:drug effects* / Q000254:growth & development
D007476:Ionophores; D000001:Calcimycin; D015759:Ionomycin; D002118:Calcium