Collaborative work to evaluate toxicity on male reproductive organs by repeated dose studies in rats 20). Testicular toxicity of nitrofurazone after 2 and 4 weeks.
Nitrofurazone (NF) has been previously demonstrated to induce testicular toxicity with 4 weeks of oral administration in rats. In the present study, rats were administered NF to assess whether testicular toxicity becomes evident with a 2-week administration period. Male Sprague-Dawley rats were administered oral doses of NF at 50 mg/kg for 2 or 4 weeks. Another group was administered NF at 100 mg/kg for 2 weeks. The control animals received the vehicle (0.5% methylcellulose) for 4 weeks. Organ weights of the testis and epididymis were significantly decreased in all NF-administered animals, and seminiferous tubules were severely atrophied, due to a total absence of spermatids and degeneration and desquamation of spermatocytes. In the epididymis, decreased numbers of spermatozoa were evident in the ducts. In rats that were administered NF at 50 mg/kg, the changes in the epididymis in the 2-week group were less prominent than those in the 4-week group. In the testis, however, the changes were similar in both groups. Thus it was demonstrated that NF-induced testicular toxicity comparable to that observed after 4 weeks of administration is also detectable after 2 weeks.
Ito
K
K
Safety Research Laboratories, Yamanouchi Pharmaceutical Co., Ltd., 1-1-8 Azusawa, Itabashi-ku, Tokyo 174-8511, Japan.
Sasaki
S
S
Yoshida
K
K
Nii
A
A
Okamiya
H
H
Sakai
T
T
D000284:Administration, Oral; D000818:Animals; D000891:Anti-Infective Agents, Local / Q000008:administration & dosage / Q000633:toxicity*; D001284:Atrophy / Q000139:chemically induced / Q000473:pathology; D001835:Body Weight / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004822:Epididymis / Q000187:drug effects / Q000473:pathology; D008297:Male; D009043:Motor Activity / Q000187:drug effects; D009583:Nitrofurazone / Q000008:administration & dosage / Q000633:toxicity*; D009929:Organ Size / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D012670:Seminiferous Epithelium / Q000187:drug effects / Q000473:pathology; D013737:Testis / Q000187:drug effects* / Q000473:pathology; D013997:Time Factors; D018675:Toxicity Tests; D014344:Trypanocidal Agents / Q000008:administration & dosage / Q000633:toxicity*
D000891:Anti-Infective Agents, Local; D014344:Trypanocidal Agents; D009583:Nitrofurazone