title: [Neurotoxicity of antibody against motoneuron on cultured rat cortical neurons].
abstract: OBJECTIVE
  In order to determine the role of antibody against motoneuron in the process of neuronal death.
  
  
  METHODS
  Cultured rat cortical neurons growing in serum-free medium, were employed to observe the effects of antibody against motoneuron (anti-SMN) on the survival of these cultured neurons.
  
  
  RESULTS
  (1) Exposure of the cultures in serum-free medium to anti-SMN with dilution of 1:50 results in death of 31%-41% of neurons for 24 hours incubation, 50%-67% for 48 hours and above 90% after 72 hours. The neurotoxicity induced by anti-SMN was accompanied by concentration-dependent release of lactate dehydrogenase into the culture medium. Cultures exposed to normal rat IgG as controls exhibited no such sign. (2) The cultured neurons exposed to anti-SMN (1:200) expressed markedly higher levels of immunoreactive Calbindin-D28k especially after 48 hours incubation with it. There was a reduction in the number of acetylcholinesterase-positive neurons compared to the normal control.
  
  
  CONCLUSIONS
  The anti-SMN could directly initiate the process of cortical neuronal death and this effect was independent of complement. The alteration of Calbindin-D28k immunoreactivity implied that calcium may be involved in the neurotoxicity induced by anti-SMN.
authors.affiliation: Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005.
authors.forename: Y
authors.initials: Y
authors.lastname: Gao
authors.forename: Y
authors.initials: Y
authors.lastname: Li
authors.forename: J
authors.initials: J
authors.lastname: Lin
authors.forename: L
authors.initials: L
authors.lastname: Li
authors.forename: X
authors.initials: X
authors.lastname: Wan
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D001323:Autoantibodies / Q000276:immunology*; D064092:Calbindin 1; D064026:Calbindins; D002470:Cell Survival; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology*; D009046:Motor Neurons / Q000276:immunology*; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D064030:S100 Calcium Binding Protein G / Q000378:metabolism
chemical_list: D001323:Autoantibodies; C576500:Calb1 protein, rat; D064092:Calbindin 1; D064026:Calbindins; D009419:Nerve Tissue Proteins; D064030:S100 Calcium Binding Protein G; D000110:Acetylcholinesterase