Genotypic and phenotypic characterization of Staphylococcus aureus strains isolated in subjects with atopic dermatitis. Higher prevalence of exfoliative B toxin production in lesional strains and correlation between the markers of disease intensity and colonization density.
Staphylococcus aureus strains generally colonize eczematous lesions of subjects with atopic dermatitis much more frequently than in the skin of normal individuals. The aim of this study was to provide a detailed genotypic and phenotypic analysis of S. aureus strains colonizing four different sites (lesional and non-lesional skin areas, nasal and pharyngeal mucosas) of 49 patients with atopic dermatitis. The 88 isolates were analyzed in duplicate by pulsed field gel electrophoresis and in their exfoliative toxin A or B production by latex test. The patients were characterized by age, sex, severity scoring of atopic dermatitis and serum eosinophil cationic protein. Fourteen (28.6%) of the patients were completely negative for S. aureus while 35 (71.4%) were positive in at least one site. The severity scores and eosinophil cationic protein levels were significantly correlated variables (P<0.001), linked to the colonization intensity (P ranging between 0.05 and <0.001 depending on the site) and to the number of colonized sites (P at least <0.01). The genotypic patterns, widely heterogeneous, showed no restriction to peculiar patterns. Only eight strains produced exfoliative toxin B which was significantly restricted to the lesional isolates (P=0.012).
Capoluongo
E
E
Laboratory of Clinical Pathology and Microbiology, Istituto San Gallicano, Rome, Italy. ecapoluongo@rm.unicatt.it
Giglio A
A
A
Lavieri
M M
MM
Lesnoni-La Parola
I
I
Ferraro
C
C
Cristaudo
A
A
Belardi
M
M
Leonetti
F
F
Mastroianni
A
A
Cambieri
A
A
Amerio
P
P
Ameglio
F
F
D000293:Adolescent; D000328:Adult; D001798:Blood Proteins / Q000378:metabolism; D002648:Child; D002675:Child, Preschool; D015169:Colony Count, Microbial; D003876:Dermatitis, Atopic / Q000097:blood / Q000382:microbiology*; D016521:Electrophoresis, Gel, Pulsed-Field; D047091:Eosinophil Granule Proteins; D005083:Exfoliatins / Q000096:biosynthesis; D005260:Female; D005838:Genotype; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D012260:Ribonucleases; D013211:Staphylococcus aureus / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity*
D001798:Blood Proteins; D047091:Eosinophil Granule Proteins; D005083:Exfoliatins; D012260:Ribonucleases