[In vitro assay method for augmention of anti-Candida activity of murine bone marrow cells by cytokines].
It is important to develop a sensitive assay method for evaluation of immunomodulating candidates including granulocyte-colony stimulating factor (G-CSF) which may augment host defense function against opportunistic infections. Effects of CSFs on anti-Candida activity of bone marrow cells (BMC) obtained from normal or cyclophosphamide-treated immunocompromised mice (CY-mice) were investigated. When BMC from CY-mice were preincubated for 24 hours in the presense of more than 0.005 ng/ml of G-CSF, anti-Candida activity was enhanced at 160 of effector (BMC) to target (C. albicans) ratio, whereas anti-Candida activity of BMC from CY-untreated mice was not changed at concentrations less than only by more than 0.5 ng/ml of G-CSF. Similar enhancement of activity was also observed in the presence of more than 0.05 ng/ml of granulocyte-macrophage colony stimulating factor (GM-CSF) in this assay using BMC from CY-mice. These findings suggest that this in vitro assay method can evaluate the activity of G-CSF and GM-CSF to augment leukocyte's anti-Candida activity.
Morofuji
S
S
Department of Microbiology and Immunology, Teikyo University School of Medicine.
Abe
S
S
Tansho
S
S
Ono
Y
Y
Yamaguchi
H
H
Okinaga
K
K
D000818:Animals; D001854:Bone Marrow Cells / Q000382:microbiology*; D002176:Candida albicans / Q000187:drug effects* / Q000254:growth & development*; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D066298:In Vitro Techniques; D007962:Leukocytes / Q000502:physiology; D051379:Mice; D008813:Mice, Inbred ICR
D016179:Granulocyte Colony-Stimulating Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor