title: Cell wall-active antibiotic induced proteins of Staphylococcus aureus identified using a proteomic approach.
abstract: Proteins produced in elevated amounts in response to oxacillin challenge of Staphylococcus aureus strain RN450, were studied by comparing Coomassie blue stained two-dimensional gels of cellular proteins. At least nine proteins were produced in elevated amounts following exposure to growth inhibitory concentrations of oxacillin. N-terminal sequences were obtained for five of the proteins and the databases were searched to tentatively identify them. The proteins were identified as homologs of (i) methionine sulfoxide reductase (MsrA); (ii) a signal transduction protein (TRAP) involved in regulating RNAIII production encoded by the agr locus; (iii) transcription elongation factor GreA; (iv) the heat shock protein GroES; and (v) the enzyme IIA component of the phosphoenolpyruvate:sugar phosphotransferase system. A similar induction response was observed with the other cell wall-active antibiotics, but not with antibiotics that affect other cellular targets. Increased transcription of the msrA and groEL genes in response to cell wall-active antibiotics was also demonstrated. Although net protein synthesis is inhibited subsequent to inhibition of peptidoglycan biosynthesis by cell wall-active antibiotics, some proteins are induced in S. aureus, presumably in an attempt by the cell to counter the inhibitory effects of these agents.
authors.affiliation: Microbiology Group, Department of Biological Sciences, Illinois State University, Normal, IL 61790-4120, USA.
authors.forename: V K
authors.initials: VK
authors.lastname: Singh
authors.forename: R K
authors.initials: RK
authors.lastname: Jayaswal
authors.forename: B J
authors.initials: BJ
authors.lastname: Wilkinson
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002473:Cell Wall / Q000187:drug effects* / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D010068:Oxacillin / Q000494:pharmacology*; D010406:Penicillins / Q000494:pharmacology*; D020543:Proteome; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D010406:Penicillins; D020543:Proteome; D010068:Oxacillin