title: Revisiting retinoblastoma protein phosphorylation during the mammalian cell cycle.
abstract: It is widely accepted that phosphorylation of the retinoblastoma (Rb) protein during the G1 phase of the mammalian division cycle is a major control element regulating passage of cells into S phase and through the division cycle. The experiments supporting G1-phase-specific Rb phosphorylation and the historical development of this idea are reviewed. By making a rigorous distinction between 'growth cessation' and the phenomena of 'cell cycle exit' or 'G1-phase arrest', the evidence for the G1-phase-specific phosphorylation of Rb protein is reinterpreted. We show that the evidence for G1-phase phosphorylation of Rb rests on few experiments and a chain of reasoning with some weak links. Evidence is reviewed that growth conditions regulate the phosphorylation of Rb. A growth-regulated control system that is independent of the cell cycle explains much of the evidence adduced to support cycle-specific phosphorylation of Rb. We propose that additional experimental evidence is needed to decide whether there is a G1-phase-specific phosphorylation of Rb protein.
authors.affiliation: Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor 48109-0620, USA. cooper@umich.edu
authors.forename: S
authors.initials: S
authors.lastname: Cooper
authors.forename: J A
authors.initials: JA
authors.lastname: Shayman
mesh_terms: D000818:Animals; D002453:Cell Cycle; D002455:Cell Division; D002472:Cell Transformation, Viral; D016193:G1 Phase; D006801:Humans; D007700:Kinetics; D008322:Mammals; D010766:Phosphorylation; D016160:Retinoblastoma Protein / Q000378:metabolism*; D013539:Simian virus 40 / Q000235:genetics
chemical_list: D016160:Retinoblastoma Protein