Plaque mineral induction and inhibition properties in the formation of supragingival calculus.
Several individual species of dental plaque bacteria have the ability to initiate the precipitation of calcium phosphate minerals in vitro; other plaque components have been shown to inhibit mineralisation. We have examined subjects' overall plaque mineralisation promoter and inhibitor properties, and have attempted to correlate them with supragingival calculus development over 6 months. Three-day-old plaque was collected from 22 adult subjects at the start and end of the study. To detect promoter activity, the plaque was placed in a suspension of brushite, the liquid phase of which was supersaturated with respect to hydroxyapatite. The extent of mineralisation was determined by the rise in phosphate concentration over 4 days. To detect inhibitor activity, plaque was placed in a similar suspension, which also contained hydroxyapatite. Promoter activity was compared with that hydroxyapatite, and inhibitor activity was compared with polyaspartate. The subjects' teeth were scaled at the start of the study, and calculus deposition was measured at the end using the Volpe Manhold method. Most plaque samples showed some promoter or inhibitor activity, or both, but no significant correlation existed between these activities and a subject's development of calculus. A significant inverse correlation existed between plaque mineralisation promoter activity and its inhibitor activity at the start of the study. Our results suggest that the nucleating and mineralisation inhibitory properties of young plaque will probably not be a useful target for a practical preventive methodology for supragingival calculus.
Pearce
E I
EI
Dental Research Group, Department of Pathology and Molecular Medicine, Wellington School of Medicine, PO Box 7343, Wellington South.
Gordon
J A
JA
Sissons
C H
CH
D000328:Adult; D000368:Aged; D002130:Calcium Phosphates / Q000819:agonists / Q000037:antagonists & inhibitors / Q000378:metabolism*; D003460:Crystallization; D003728:Dental Calculus / Q000737:chemistry / Q000209:etiology / Q000378:metabolism*; D003773:Dental Plaque / Q000737:chemistry / Q000150:complications / Q000378:metabolism*; D003864:Depression, Chemical; D005260:Female; D006801:Humans; D006882:Hydroxyapatites / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D018709:Statistics, Nonparametric; D013268:Stimulation, Chemical
D002130:Calcium Phosphates; D006882:Hydroxyapatites; C494366:calcium phosphate, dibasic, dihydrate