[HIM82 protects RBC from the ROS damage produced by activated PMN].
OBJECTIVE
To investigate the protection of RBC from ROS damage produced by activated PMN with McAb-HIM82.


METHODS
RBC was injured by ROS produced from PMN activated with PMA and the injury was antagonised by adding HIM82(30 micrograms/ml) to the medium with PMN.


RESULTS
Comparing antagonitic group with injured group, the activity of AchE on RBC membrane after adding HIM82 was recovered from (71.36 +/- 22.15)% to (87.66 +/- 14.78)% (n = 6 P < 0.001). The proportion of MDA was decreased from (52.42 +/- 20.63)% to (23.81 +/- 12.4)%(n = 9 P < 0.001); the hemolysis rate was decreased from (80.76 +/- 20.23)% to (66.30 +/- 7.08)% (n = 6 P < 0.05);


CONCLUSIONS
The result showed that the McAb HIM82 could protect RBC from injury by down-reguration of ROS level in activated PMN. It could help to establish a newer approach to study the regulation effect of McAb HIM82 on ROS production from PMN actived with relerant cytokines as well as mediators.
Guo
H
H
Institute of Hematology, CAMS and PUMC, Tianjin 300020.
Fa
X
X
Tian
P
P
Yang
L
L
D000110:Acetylcholinesterase / Q000378:metabolism*; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D004910:Erythrocyte Membrane / Q000201:enzymology*; D006801:Humans; D008315:Malondialdehyde / Q000378:metabolism; D009504:Neutrophils / Q000502:physiology*; D017382:Reactive Oxygen Species / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D017382:Reactive Oxygen Species; D008315:Malondialdehyde; D000110:Acetylcholinesterase