Structure of the O-specific polysaccharide of Vibrio cholerae O9 containing 2-acetamido-2-deoxy-D-galacturonic acid.
The O-specific polysaccharide (OPS) was isolated by mild-acid degradation of the lipopolysaccharide of Vibrio cholerae O9 and studied by carboxyl reduction, sugar and methylation analyses, Smith degradation, and two-dimensional NMR spectroscopy, including COSY, TOCSY, NOESY, and H-detected 1H,(13)C HMQC experiments. The following structure of the pentasaccharide-repeating unit of the OPS was established:
Kocharova
N A
NA
Karolinska Institute, Clinical Research Centre, Huddinge University Hospital, Novum, 141 86, Huddinge, Sweden.
Knirel
Y A
YA
Jansson
P
P
Weintraub
A
A
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D006603:Hexuronic Acids / Q000737:chemistry*; D008969:Molecular Sequence Data; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D019081:O Antigens / Q000737:chemistry* / Q000302:isolation & purification; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000302:isolation & purification; D014734:Vibrio cholerae / Q000737:chemistry*
C439356:2-acetamido-2-deoxy-galacturonic acid; D006603:Hexuronic Acids; D019081:O Antigens; D011135:Polysaccharides, Bacterial