Ubiquitin and the SCF(Grr1) ubiquitin ligase complex are involved in the signalling pathway activated by external amino acids in Saccharomyces cerevisiae.
In response to external amino acids, the permease-like sensor Ssy1 of Saccharomyces cerevisiae activates a pathway leading to transcriptional induction of several permease genes including AGP1 and PTR2. We previously reported that AGP1 induction requires Grr1, the F-box protein part of the SCF(Grr1) ubiquitin-ligase complex. We show here that ubiquitin, other components of SCF(Grr1) and the ubiquitin-conjugating enzyme Cdc34 are essential for AGP1 and PTR2 induction. This suggests that transduction to these genes of the amino acid signal generated by Ssy1 involves an SCF(Grr1)-catalysed ubiquitination step.
Bernard
F
F
Laboratoire de Physiologie Cellulaire, Université Libre de Bruxelles CP 300, Institut de Biologie et de Médecine Moléculaires, rue des Pr. Jeener et Brachet 10, 6041, Gosselies, Belgium.
André
B
B
D026905:Amino Acid Transport Systems; D000596:Amino Acids / Q000737:chemistry; D064173:Anaphase-Promoting Complex-Cyclosome; D002352:Carrier Proteins / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D044783:F-Box Proteins; D005656:Fungal Proteins / Q000378:metabolism* / Q000502:physiology; D017353:Gene Deletion; D005838:Genotype; D047908:Intracellular Signaling Peptides and Proteins; D008025:Ligases / Q000502:physiology; D008565:Membrane Proteins / Q000096:biosynthesis / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins; D015398:Signal Transduction; D013997:Time Factors; D014158:Transcription, Genetic; D014161:Transduction, Genetic; D044763:Ubiquitin-Conjugating Enzymes; D043743:Ubiquitin-Protein Ligase Complexes; D044767:Ubiquitin-Protein Ligases; D014452:Ubiquitins / Q000378:metabolism* / Q000502:physiology; D001616:beta-Galactosidase / Q000378:metabolism
D026905:Amino Acid Transport Systems; D000596:Amino Acids; D002352:Carrier Proteins; D044783:F-Box Proteins; D005656:Fungal Proteins; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; C093495:PTR2 protein, S cerevisiae; C120527:SSY1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014452:Ubiquitins; C071032:GRR1 protein, S cerevisiae; C577449:CDC34 protein, S cerevisiae; D044763:Ubiquitin-Conjugating Enzymes; D043743:Ubiquitin-Protein Ligase Complexes; D064173:Anaphase-Promoting Complex-Cyclosome; D044767:Ubiquitin-Protein Ligases; D001616:beta-Galactosidase; D008025:Ligases