title: [Immune response induced by hepatitis B DNA vaccine pCI-S-IRES- ProT alpha].
abstract: OBJECTIVE
  To investigate immune response in mice inoculated with DNA vaccine.
  
  
  METHODS
  pCI-S and pCI-S-IRES- ProT alpha were constructed by gene technology, transferred into cell line NIH-3T3 mediated by lipofectamine. Mice were inoculated with these plasmids too. HBsAg and HBsAb were detected by ELISA, and transcriptions of these plasmids were detected by RT-PCR.
  
  
  RESULTS
  pCI-S -IRES- ProT alpha, and pCI-S were effectively expressed in cultured cells and vaccinated animals. Humoral immune and specific T-cell proliferative responses were stronger in pCI-S-IRES group than pCI-S group.
  
  
  CONCLUSIONS
  Plasmids coexpression of prothymosin alpha and hepatitis B surface antigen can improve immune responses significantly.
authors.affiliation: Department of Infectious Diseases, Ruijin Hospital, Shanghai 200025, China.
authors.forename: B
authors.initials: B
authors.lastname: Wang
authors.forename: X
authors.initials: X
authors.lastname: Zhou
authors.forename: J
authors.initials: J
authors.lastname: Zhu
authors.forename: Q
authors.initials: Q
authors.lastname: Xie
authors.forename: G
authors.initials: G
authors.lastname: Zang
authors.forename: H
authors.initials: H
authors.lastname: Yu
authors.forename: Q
authors.initials: Q
authors.lastname: Guo
mesh_terms: D000818:Animals; D006514:Hepatitis B Surface Antigens / Q000032:analysis; D017325:Hepatitis B Vaccines / Q000276:immunology*; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019444:Vaccines, DNA / Q000276:immunology*
chemical_list: D006514:Hepatitis B Surface Antigens; D017325:Hepatitis B Vaccines; D019444:Vaccines, DNA