Neutrophil infiltration is involved in 5-fluorouracil induced lipid peroxidative damage of the small intestine.
This study evaluated the mechanisms of 5-fluorouracil (5-FU) induced intestinal damage by investigating the lipid peroxide (LPO) level, myeloperoxidase (MPO) activity and disaccharidase activity in rats. Group I animals (n = 6) were sacrificed and served as a normal control group. Group II animals (n = 24) were given a single intraperitoneal injection of 5-FU (150 mg/kg) and every 8 rats were sacrificed on day 1, 3 and 5 after injection respectively.


RESULTS
LPO concentration in blood and intestinal mucosa was significantly higher in the group II than in the group I on day 1 and 3 (P < 0.05). MPO activity was significantly higher in the group II than in the group I at different times (P < 0.01). Lactase activity on day 5 (P < 0.01); sucrase activity (P < 0.01 and P < 0.05 respectively) and maltase activity (P < 0.01) on day 3 and 5, were significantly lower in the group II than in the group I.


CONCLUSIONS
The results indicate that neutrophil infiltration may be involved in 5-FU-induced lipid peroxidative damage of the small intestine which was reflected by the decreased disaccharidases activities.
Chen
D
D
Department of Gastroenteric Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an 710032.
Wu
G
G
Wang
W
W
Wang
J
J
D000818:Animals; D000963:Antimetabolites / Q000494:pharmacology*; D004186:Disaccharidases / Q000378:metabolism; D005472:Fluorouracil / Q000494:pharmacology*; D007413:Intestinal Mucosa / Q000378:metabolism / Q000473:pathology; D007421:Intestine, Small / Q000378:metabolism* / Q000473:pathology; D008054:Lipid Peroxides / Q000378:metabolism*; D008297:Male; D009504:Neutrophils / Q000502:physiology*; D009195:Peroxidase / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013393:Sucrase / Q000378:metabolism; D000520:alpha-Glucosidases / Q000378:metabolism; D001616:beta-Galactosidase / Q000378:metabolism
D000963:Antimetabolites; D008054:Lipid Peroxides; D009195:Peroxidase; D004186:Disaccharidases; D000520:alpha-Glucosidases; D001616:beta-Galactosidase; D013393:Sucrase; D005472:Fluorouracil