[The effect of antioxidant sodium ferulate on human lymphocytes apoptosis induced by H2O2].
OBJECTIVE
Sodium ferulate is a potent antioxidant purified from ferula assafoetida L. The effect of sodium ferulate on apoptosis of human lymphocytes induced by H2O2 in vitro was studied.


METHODS
Apoptosis was detected by light and electron microscopic, DNA electrophoresis and Flow cytometric cell cycle analysis. mitochondrial function was detected by MTT method. BCL-2 and Bax expression was detected by confocal laser scanning microscope.


RESULTS
The morphological and biochemical changes characteristic of apoptosis were partially restored after treatment with sodium ferulate, Immunofluorescence assay showed a decrease in BCL-2 and an increase in Bax oncogene proteins in lymphocytes treated with H2O2 and an improvement of cell apoptosis was found after sodium ferulate treatment.


CONCLUSIONS
Oxidative stress can induce apoptosis in lymphocytes, and this induction can be partly prevented by sodium ferulate.
Lu
Y
Y
Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005.
Xu
C
C
Yang
Y
Y
Pan
H
H
D017209:Apoptosis / Q000187:drug effects*; D003373:Coumaric Acids / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000494:pharmacology*; D006801:Humans; D006861:Hydrogen Peroxide; D008214:Lymphocytes / Q000166:cytology*; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis; D051028:bcl-2-Associated X Protein
C494826:BAX protein, human; D003373:Coumaric Acids; D016166:Free Radical Scavengers; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; C004999:ferulic acid; D006861:Hydrogen Peroxide