title: Immunohistochemical evaluation of cathepsin D expression in colorectal cancer.
abstract: Cathepsin D is one of the main proteolytic enzymes contributing to the development of cancer. The aim of the present study was to evaluate the expression of cathepsin D in 48 colorectal adenocarcinomas at pT3 stage of clinical advancement and G2 histologic grade. The correlation between cathepsin D expression, anatomo-clinical advancement and the presence of chosen anatomo-clinical properties of the tumours was also analysed. Formalin-fixed and paraffin-embedded tissues were investigated with anti-cathepsin D antibody. Immunolocalisation of cathepsin D was performed using Labelled Streptavidin Biotin (LSAB) method. A statistical correlation was found between high catepsin D expression in the cells of the main mass of the cancer and low cathepsin D expression in low-differentiated cancer cells which formed nests at the border of cancer invasion. There was no correlation between cathepsin D expression in the cells of colorectal cancer and other anatomo-clinical parameters of the tumours.
authors.affiliation: Department of Physiology, Medical Academy, Białystok, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Chabowski
authors.forename: M
authors.initials: M
authors.lastname: Sulkowska
authors.forename: M
authors.initials: M
authors.lastname: Sulkowski
authors.forename: W
authors.initials: W
authors.lastname: Famulski
authors.forename: E
authors.initials: E
authors.lastname: Skrzydlewska
authors.forename: W
authors.initials: W
authors.lastname: Kisielewski
mesh_terms: D000230:Adenocarcinoma / Q000201:enzymology* / Q000473:pathology; D000368:Aged; D002402:Cathepsin D / Q000096:biosynthesis*; D015179:Colorectal Neoplasms / Q000201:enzymology* / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D016612:Paraffin Embedding; D016707:Tissue Fixation
chemical_list: D002402:Cathepsin D