Restriction enzyme and fluorochrome banding analysis of the constitutive heterochromatin of Saguinus species (Callitrichidae, Primates).
Metaphases of Saguinas fuscicollis fuscicollis and Saguinas mystax were subjected to restriction enzyme banding (Alu I, Hae III, Hin fI, Rsa I, Dde I, Mbo I and Msp I) and sequenced C-banding, together with fluorochrome staining (CMA3 and DAPI). Both species showed large C-bands in the pericentromeric regions. S. f. fuscicollis also manifested distal C-bands in both arms of pair 5 and in the short arms of pairs 8-15. In each species the heterochromatin revealed different reactions to the restriction enzymes and fluorochromes. This was related to its location in the genome (centromeric, pericentromeric, distal), making possible the identification of distinct categories of constitutive heterochromatin. In S. f. fuscicollis there were at least five types, namely centromeric in bi-armed chromosomes, centromeric in acrocentrics, pericentromeric, distal, and cryptic bands, detected only with the Alu I. There were three types in S. mystax, viz centromeric in bi-armed chromosomes, centromeric in acrocentric, and pericentromeric chromosomes. Several aspects of their constitution and origin are discussed.
Pieczarka
J C
JC
Departamento de Genetica, CCB, Universidade Federal do Pará, Belém, Brazil.
Nagamachi
C Y
CY
Muniz
J A
JA
Barros
R M
RM
Mattevi
M S
MS
D000818:Animals; D005456:Fluorescent Dyes / Q000378:metabolism; D006570:Heterochromatin / Q000235:genetics* / Q000378:metabolism; D007211:Indoles / Q000378:metabolism; D007364:Intercalating Agents / Q000378:metabolism; D007621:Karyotyping; D008856:Microscopy, Fluorescence; D015183:Restriction Mapping; D012452:Saguinus / Q000235:genetics* / Q000502:physiology
D005456:Fluorescent Dyes; D006570:Heterochromatin; D007211:Indoles; D007364:Intercalating Agents; C007293:DAPI