[A new rat brain-glioma model].
OBJECTIVE
To build a new rat brain glioma model with LacZ as a reporter gene.


METHODS
Plasmid BAG was transfected into C6 glioma cell. The cell cycle, cell proliferation, malignant infiltrative ability of wild and transfected glioma cell were observed. Rat glioma model was built using stereotaxic implantation.


RESULTS
There was no difference between transfected and wild glioma cell in cell growth characteristics and tumorigenesis.


CONCLUSIONS
It was easy to observe the infiltrative area on cellular level from the rat glioma model made by transfected tumor cell. The method is satisfactory for radiation and chemotherapeutic trials of glioma.
Xing
B
B
PUMC Hospital, CAMS and PUMC, Beijing 100730.
Ren
Z
Z
Su
C
C
D000818:Animals; D001932:Brain Neoplasms / Q000235:genetics / Q000473:pathology*; D004195:Disease Models, Animal; D017930:Genes, Reporter; D005910:Glioma / Q000235:genetics / Q000473:pathology*; D007763:Lac Operon; D009368:Neoplasm Transplantation; D051381:Rats; D017208:Rats, Wistar; D012190:Retroviridae / Q000235:genetics; D014162:Transfection; D014407:Tumor Cells, Cultured