title: Gene profile of replicative senescence is different from progeria or elderly donor.
abstract: In vitro cellular senescence of human diploid fibroblast has been a good model for aging research, which shows similar phenotypes to in vivo aging. Gene expression profiling would provide an insight to understand the mechanism of senescence. Using cDNA microarray containing 384 known genes, we compared the expression profiles of three different types of aging models: replicative senescence, fibroblasts from progeria or from elderly donor. Although all of them showed senescence phenotypes, distinct sets of genes were altered in each group. Pairwise plots or cluster analysis of activation fold of gene expression revealed closer relationships between fibroblasts from progeria or from old individual, but not between replicative senescence fibroblasts and either models. Differential expression pattern of several genes were confirmed by RT-PCR. We suggest that the replicative senescence model might behave differently to other types of aging models due to the distinct gene expression.
authors.affiliation: Department of Biochemistry and Molecular Biology, Seoul National University, Seoul, Korea.
authors.forename: W Y
authors.initials: WY
authors.lastname: Park
authors.forename: C I
authors.initials: CI
authors.lastname: Hwang
authors.forename: M J
authors.initials: MJ
authors.lastname: Kang
authors.forename: J Y
authors.initials: JY
authors.lastname: Seo
authors.forename: J H
authors.initials: JH
authors.lastname: Chung
authors.forename: Y S
authors.initials: YS
authors.lastname: Kim
authors.forename: J H
authors.initials: JH
authors.lastname: Lee
authors.forename: H
authors.initials: H
authors.lastname: Kim
authors.forename: K A
authors.initials: KA
authors.lastname: Kim
authors.forename: H J
authors.initials: HJ
authors.lastname: Yoo
authors.forename: J S
authors.initials: JS
authors.lastname: Seo
mesh_terms: D000368:Aged; D000375:Aging / Q000235:genetics* / Q000378:metabolism; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000235:genetics; D005347:Fibroblasts / Q000378:metabolism; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D011371:Progeria / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis
chemical_list: D012333:RNA, Messenger