title: Peritoneal lavage with oxygenated perfluorochemical preserves intestinal mucosal barrier function after ischemia-reperfusion and ameliorates lung injury.
abstract: OBJECTIVE
  To evaluate the effect of peritoneal lavage with an oxygenated perfluorochemical (PFC) on intestinal ischemia-reperfusion injury (IIR), we assessed intestinal barrier function in terms of bacterial translocation and endotoxemia, morphologic changes, and changes of intestinal luminal pH in rats subjected to IIR. We also examined lung injury after IIR to test the effect of oxygenated PFC lavage on remote organ failure.
  
  
  METHODS
  Prospective, randomized, and controlled animal study.
  
  
  METHODS
  Laboratory of a university hospital.
  
  
  METHODS
  Male Sprague-Dawley rats.
  
  
  METHODS
  Rats were subjected to ischemia by clipping the superior mesenteric artery. Reperfusion was achieved by release of the clip. Lavage of the abdominal cavity was performed by inflow and outflow of oxygenated PFC solution during ischemia.
  
  
  RESULTS
  Rats undergoing peritoneal lavage with oxygenated PFC (PFC group) showed significantly better survival after IIR. The frequency of bacterial translocation and the endotoxin concentration in superior mesenteric venous blood were significantly lower in the PFC group. Luminal acidosis also was alleviated in the PFC group. Furthermore, PFC lavage preserved the intestinal mucosal architecture and inhibited interstitial edema and infiltration of inflammatory cells in the lungs.
  
  
  CONCLUSIONS
  We conclude that peritoneal lavage with oxygenated PFC protects the intestinal mucosa and maintains mucosal barrier function after IIR. Preservation of the intestinal mucosa ameliorates lung injury after IIR.
authors.affiliation: Department of Surgery II, Hamamatsu University School of Medicine, Hamamatsu, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Ohara
authors.forename: N
authors.initials: N
authors.lastname: Unno
authors.forename: H
authors.initials: H
authors.lastname: Mitsuoka
authors.forename: H
authors.initials: H
authors.lastname: Kaneko
authors.forename: S
authors.initials: S
authors.lastname: Nakamura
mesh_terms: D000818:Animals; D018988:Bacterial Translocation; D001835:Body Weight; D004731:Endotoxins / Q000097:blood; D005466:Fluorocarbons / Q000008:administration & dosage / Q000627:therapeutic use*; D007082:Ileum / Q000473:pathology; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000382:microbiology; D008168:Lung / Q000473:pathology; D008297:Male; D009929:Organ Size; D010533:Peritoneal Lavage / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000628:therapy*
chemical_list: D004731:Endotoxins; D005466:Fluorocarbons