title: [Experimental study on endothelialization of autologous pericardial heart valves in vitro].
abstract: OBJECTIVE
  To investigate a method of autologous pericardial heart valves (APHV) treatment beneficial to endothelialization.
  
  
  METHODS
  Bovine aortic endothelium cells (BAEC) were cultured and seeded in autologous pericardial pieces treated with different methods in vitro. The influence of different treatment on cellular growing, amount of 3H-TdR incorporation and PGI2 releasing, contents of intracellular cAMP was studied by means of transmission electron microscopy, 3H-TdR incorporation experimentation and radioimmunity method.
  
  
  RESULTS
  The BAEC seeded in bovine pericardial pieces treated with three different concentrations of GA 10 minutes could alive. In the group with method of treatment with 0.2% GA, the growing of BAEC was best, the cellular density and the amount of 3H-TdR incorporation at different time spots were higher than that of the other groups and it did not enhance the abnormal synthesis of cAMP and PGI2 in BAEC.
  
  
  CONCLUSIONS
  The pericardium treated with 0.2% GA 10 minutes has little toxicity to endothelium cell, which indicates that this method is beneficial to endothelialization of APHV postoperatively.
authors.affiliation: Institute of Thoracic and Cardiovascular Surgery, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
authors.forename: Z
authors.initials: Z
authors.lastname: Song
authors.forename: Z
authors.initials: Z
authors.lastname: Xu
authors.forename: B
authors.initials: B
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001705:Bioprosthesis; D002417:Cattle; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects*; D005976:Glutaral / Q000633:toxicity*; D006350:Heart Valve Prosthesis; D066298:In Vitro Techniques; D010496:Pericardium / Q000166:cytology / Q000187:drug effects*; D013997:Time Factors
chemical_list: D005976:Glutaral