[Cloning of human papillomavirus type 16 E7 gene and expression in E. coli].
OBJECTIVE
To study the biological activity of the E7 gene of human papillomavirus 16 (HPV16).


METHODS
E7 gene from the cervical carcinoma of Chinese women was amplified by the PCR. The E7 DNA was sequenced and compared with the prototype E7 gene of HPV 16.


RESULTS
It showed no mutations. However when E7 gene was inserted into the prokaryotic expression vector pGEX-2T and the fused E7 protein was efficiently expressed in E. Coli (DH5 alpha). The E7 antibody could combine specifically with this fused protein by Western blot technique.


CONCLUSIONS
The result might aid for epidemiological investigation and preparation of the vaccine.
Liu
C
C
Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005.
Si
J
J
Liu
S
S
Xu
X
X
Song
G
G
D001483:Base Sequence; D004926:Escherichia coli / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D005814:Genes, Viral; D006801:Humans; D008969:Molecular Sequence Data; D009856:Oncogene Proteins, Viral / Q000096:biosynthesis / Q000235:genetics*; D027383:Papillomaviridae / Q000235:genetics*; D050725:Papillomavirus E7 Proteins; D002583:Uterine Cervical Neoplasms / Q000821:virology*
D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; C059731:oncogene protein E7, Human papillomavirus type 16