Preliminary studies by ELISA on the antigen and antibody dynamics in the early stages of experimental infections with Trypanosoma evansi in cattle.
Six 6-month-old bulls were experimentally infected with five different isolates of Trypanosoma evansi; two received the same isolate and the other four received different isolates. The parasitaemias and serum antigen levels were monitored regularly by the haematocrit centrifuge technique (HCT) and antigen-detection ELISA (Ag-ELISA), respectively. Trypanosomal antigen was demonstrated by the Ag-ELISA by 10-14 days post inoculation in four cattle, while parasitaemias were first found to be positive in individual cattle over a longer period of time post inoculation (6-28 days). In two cattle, the Ag-ELISA values were also positive when the animals were found to harbour trypanosomes by the HCT and only turned negative 3 days after treatment, while the ELISA values fluctuated during the experiment in another two bulls. The remaining two cattle never produced positive ELISA results despite positive parasitological results. The antibody titres in all six cattle started to rise around 10 days post inoculation and then stayed high throughout the experiment. It was concluded that the Ag-ELISA would produce some false negative results in the early stages of T. evansi infection owing to variations in the balance of parasitaemia and antibody levels in the circulation, and in the pathogenicity of parasite strains.
Thammasart
S
S
National Institute of Animal Health, Kaset-Klang, Bangkhen, Bangkok 10900, Thailand.
Kanitpun
R
R
Saithasao
M
M
Kashiwazaki
Y
Y
D000818:Animals; D000913:Antibodies, Protozoan / Q000302:isolation & purification*; D000953:Antigens, Protozoan / Q000302:isolation & purification*; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology / Q000469:parasitology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000592:standards / Q000662:veterinary; D006400:Hematocrit / Q000662:veterinary; D008297:Male; D011237:Predictive Value of Tests; D014345:Trypanosoma / Q000276:immunology* / Q000472:pathogenicity*; D014352:Trypanosomiasis / Q000276:immunology / Q000469:parasitology / Q000662:veterinary*
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan