[Effect of trichosanthin of cell cycle and apoptosis of murine melanoma cells].
OBJECTIVE
To study the inhibitory effect of purified trichosanthin component on the proliferation of malignant melanoma.


METHODS
The effect of purified trichosanthin component on the DNA synthesis, cell cycle and cell apoptosis of murine melanoma cells were detected by flowcytometry when cultured in vitro.


RESULTS
The significant G0/G1 phase arrest was revealed by the increase of cells in G0/G1 phase and decrease of cells in S phase. The obvious apoptosis of melanoma cells was induced by purified trichosanthin component. G0/G1 phase arrest was highly correlated with apoptosis (r = 0.8705).


CONCLUSIONS
The purified trichosanthin component can markedly inhibit melanoma cells by the suppression of DNA synthesis in S phase and cell mitosis as well as induction of cell apoptosis.
Bi
L
L
Third Teaching Hospital, Norman Bethune University of Medical Sciences, Changchun(130031).
Li
H
H
Zhang
Y
Y
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects; D008546:Melanoma, Experimental / Q000473:pathology*; D051379:Mice; D015978:Trichosanthin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D000972:Antineoplastic Agents, Phytogenic; D015978:Trichosanthin