title: The missense Glu298Asp variant of the endothelial nitric oxide synthase gene is strongly associated with placental abruption.
abstract: We recently identified a missense variant (Glu298Asp) that lies within exon 7 of the endothelial nitric oxide synthase (eNOS) gene, and that is associated with severe preeclampsia (proteinuric hypertension that develops as a consequence of pregnancy). Maternal hypertension is the most consistently identified factor predisposing to placental abruption. Our objective, therefore, was to analyze the association between the Glu298Asp eNOS gene variant and placental abruption. The study participants included 35 patients with histories of placental abruption and 170 control subjects. Screening for the Glu298Asp eNOS gene variant was carried out by analysis of polymerase chain reaction/restriction fragment length polymorphism. The analyses revealed that the frequency of the Glu298Asp variant (Glu298Asp homozygotes and heterozygotes) was significantly (P<0.001) higher in the placental abruption group (n=14; 40%) than in the control group (n=24; 14%). We conclude that the presence of the Glu298Asp eNOS gene variant could be a marker of increased risk of developing placental abruption.
authors.affiliation: Department of Obstetrics and Gynecology, Kumamoto University School of Medicine, Japan. yoshimur@kaiju.medic.kumamoto-u.ac.jp
authors.forename: T
authors.initials: T
authors.lastname: Yoshimura
authors.forename: M
authors.initials: M
authors.lastname: Yoshimura
authors.forename: A
authors.initials: A
authors.lastname: Tabata
authors.forename: H
authors.initials: H
authors.lastname: Yasue
authors.forename: H
authors.initials: H
authors.lastname: Okamura
mesh_terms: D000037:Abruptio Placentae / Q000201:enzymology / Q000235:genetics*; D000328:Adult; D001224:Aspartic Acid / Q000235:genetics; D004247:DNA / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D005820:Genetic Testing; D005838:Genotype; D018698:Glutamic Acid / Q000235:genetics; D006801:Humans; D020125:Mutation, Missense / Q000235:genetics*; D019001:Nitric Oxide Synthase / Q000235:genetics*; D052250:Nitric Oxide Synthase Type III; D011247:Pregnancy
chemical_list: D001224:Aspartic Acid; D018698:Glutamic Acid; D004247:DNA; C496314:NOS3 protein, human; D019001:Nitric Oxide Synthase; D052250:Nitric Oxide Synthase Type III