[Alternative approaches to genome mapping and sequencing].
The main methods used for large-scale mapping of the human and other genomes are reviewed. These methods comprise two procedures of random mapping/sequencing and an approach using linking and jumping libraries. Importantly, no method used up to now has proved efficient in comparative genome analysis. A new method is presented basing on slalom libraries. These libraries provide 10-100 times higher efficiency and may be used for mapping and sequencing whole genomes by small research groups.
Zabarovskiĭ
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Microbibology and Tumor Biology Center and Center for Genomics Research, Karolinska Institute, 17177, Stockholm, Sweden. eugzab@ki.se
D000818:Animals; D002874:Chromosome Mapping / Q000379:methods; D016678:Genome; D015894:Genome, Human; D006801:Humans; D017422:Sequence Analysis, DNA / Q000379:methods