title: Structural and functional stability of the mature transferrin receptor from human placenta.
abstract: The transferrin receptor (TfR) is a N- and O-glycosylated transmembrane protein mediating the cellular iron uptake by binding and internalization of diferric transferrin. In this study, rate constants and dissociation constants of 125I-ferri-transferrin binding to the human TfR were examined dependent on receptor glycan composition, pH, bivalent cations, and temperature. To do so, purified human placental TfR was noncovalently immobilized to polystyrene surfaces and subjected to alterations in various parameters. We found that transferrin binding was clearly dependent on a receptor pretreatment with buffers of various pH in that most of the TfR molecules irreversibly lost transferrin binding activity below pH 6.5. However, the dissociation constant of the remaining active binding sites was not affected. Similarly, we were able to define the thermal stability of the receptor as a function of transferrin binding ability. Binding of transferrin was completely lost provided that the receptor was pretreated at temperatures of at least 65 degrees C. Treatment with EDTA also caused an irreversible loss of transferrin binding activity, indicating that the functionally active conformation of the mature TfR depends on bivalent cations. In order to examine the role of the receptor glycans, we enzymatically removed the sialic acid residues, the hybrid and oligomannosidic N-glycans, or all types of N-glycans. In contrast to the parameters described above, all desialylated and N-deglycosylated TfR variants had exactly the same transferrin binding properties as the native TfR. To assess changes in the secondary structure of the receptor, circular dichroic spectra were recorded from TfR at pH 5.0, from heat pretreated receptor and from deglycosylated TfR. Since the receptor did not exhibit detectable changes in the CD spectrum of the deglycosylated receptor, it can be concluded that the N-linked carbohydrates of the mature, fully processed TfR are not essential for transferrin binding and conformational stability.
authors.affiliation: Institut für Laboratoriumsmedizin und Pathobiochemie, Medizinische Fakultät Charité der Humboldt-Universität zu Berlin, Germany.
authors.forename: G
authors.initials: G
authors.lastname: Orberger
authors.forename: H
authors.initials: H
authors.lastname: Fuchs
authors.forename: R
authors.initials: R
authors.lastname: Geyer
authors.forename: R
authors.initials: R
authors.lastname: Gessner
authors.forename: E
authors.initials: E
authors.lastname: Köttgen
authors.forename: R
authors.initials: R
authors.lastname: Tauber
mesh_terms: D001665:Binding Sites; D002118:Calcium / Q000494:pharmacology; D002412:Cations; D002846:Chromatography, Affinity; D002942:Circular Dichroism; D004305:Dose-Response Relationship, Drug; D004492:Edetic Acid / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D006031:Glycosylation; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007501:Iron / Q000493:pharmacokinetics; D007700:Kinetics; D037102:Lectins / Q000378:metabolism; D008024:Ligands; D010920:Placenta / Q000378:metabolism*; D011134:Polysaccharides / Q000494:pharmacology; D011485:Protein Binding; D011487:Protein Conformation; D011489:Protein Denaturation; D017433:Protein Structure, Secondary; D011990:Receptors, Transferrin / Q000737:chemistry* / Q000378:metabolism / Q000502:physiology*; D013696:Temperature; D013997:Time Factors; D014168:Transferrin / Q000378:metabolism / Q000493:pharmacokinetics
chemical_list: D002412:Cations; D037102:Lectins; D008024:Ligands; D011134:Polysaccharides; D011990:Receptors, Transferrin; D014168:Transferrin; D004492:Edetic Acid; D007501:Iron; D002118:Calcium