title: Transcriptional repression by suppressor of hairless involves the binding of a hairless-dCtBP complex in Drosophila.
abstract: Notch is the receptor for a conserved signaling pathway that regulates numerous cell fate decisions during development [1]. Signal transduction involves the presenilin-dependent intracellular processing of Notch and the nuclear translocation of the intracellular domain of Notch, NICD [2-6]. NICD associates with Suppressor of Hairless [Su(H)], a DNA binding protein, and Mastermind (Mam), a transcriptional coactivator [7-9]. In the absence of Notch signaling, Su(H) acts as a transcriptional repressor [10, 11]. Repression by Su(H) is relieved by the activation of Notch [12-16]. In the Drosophila embryo, this transcriptional switch from repression to activation is important for patterning the expression of the single-minded (sim) gene along the dorsoventral axis [12]. Here, we investigate the mechanisms by which Su(H) inhibits the expression of Notch target genes in Drosophila. We show that Hairless, an antagonist of Notch signaling [17-19], is required to repress the transcription of the sim gene. Hairless forms a DNA-bound complex with Su(H). Furthermore, it directly binds the Drosophila C-terminal Binding Protein (dCtBP), which acts as a transcriptional corepressor. The dCtBP binding motif of Hairless is essential for the function of Hairless in vivo. We propose that Hairless mediates transcriptional repression by Su(H) via the recruitment of dCtBP.
authors.affiliation: Ecole Normale Supérieure, Unite Mixte de Recherche 8544, 46 rue d'Ulm, 75230, Cedex 05, Paris, France.
authors.forename: V
authors.initials: V
authors.lastname: Morel
authors.forename: M
authors.initials: M
authors.lastname: Lecourtois
authors.forename: O
authors.initials: O
authors.lastname: Massiani
authors.forename: D
authors.initials: D
authors.lastname: Maier
authors.forename: A
authors.initials: A
authors.lastname: Preiss
authors.forename: F
authors.initials: F
authors.lastname: Schweisguth
mesh_terms: D000429:Alcohol Oxidoreductases; D000595:Amino Acid Sequence; D000818:Animals; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004330:Drosophila / Q000196:embryology; D029721:Drosophila Proteins; D019476:Insect Proteins / Q000378:metabolism*; D010750:Phosphoproteins / Q000378:metabolism*; D011485:Protein Binding; D012097:Repressor Proteins / Q000502:physiology*; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors; D014158:Transcription, Genetic
chemical_list: D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D019476:Insect Proteins; D010750:Phosphoproteins; D012097:Repressor Proteins; C088731:Su(H) protein, Drosophila; D014157:Transcription Factors; C076560:H protein, Drosophila; D000429:Alcohol Oxidoreductases; C096794:C-terminal binding protein