Oxidative stress before and after operation in patients with chronic cholecystitis containing gallstone.
To study the relationship of oxidative, antioxidative constituents and antioxidases in blood with chronic cholecystitis containing gallstone, levels of lipoperoxides (LPO), nitric oxide (NO), vitamin C(VC), vitamin E (VE) and beta-carotene (beta-CAR) in plasma as well as level of LPO, activities of superoxide dismulase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) in erythrocytes were investigated by spectrophotometric assay in 107 patients with this condition (PCg) and 100 healthy volunteers (HVs). Compared with HVs group, the average value of LPO and NO in plasma and that of LPO in erythrocytes of PCg group were significantly increased (P < 0.0001), while that of VC, VE and beta-CAR in plasma and the average activities of SOD, CAT and GSH-Px in erythrocytes were significantly decreased (P < 0.0001). Linear regression and correlation analysis for 107 preoperative PCg showed that the value of LPO and NO in plasma and that of LPO in erythrocytes of PCg gradually increased (P < 0.0001), representing a significant linear positive correlation. The value of VC, VE and beta-CAR in plasma and that of SOD, CAT and GSH-Px in erythrocytes of PCg gradually decreased (P < 0.0001), representing a significant linear negative correlation. Stepwise regression and correlation analysis for 107 preoperative PCg suggested that the closest correlation was observed between the course of disease and the value of NO and VC in plasma and that of SOD, GSH-Px and LPO in erythrocytes, r = 0.7306, F = 32.1408, P < 0.0001. Compared with the preoperative PCg group, the average value of LPO and NO in plasma and that of LPO in erythrocytes of the postoperative PCg group were significantly decreased (P < 0.0001). Furthermore, the average value of VC in plasma and that of SOD, CAT and GSH-Px in erythrocytes of the postoperative PCg group were significantly increased (P < 0.0001), whereas no significant difference was found between their average value of VE and beta-CAR in plasma. These findings suggested that oxidative stress was an aggravating pathological condition in PCg group. Therefore, we recommend that in treating PCg, antioxidants such as VC, VE, beta-CAR should be given in order to alleviate their potential oxidative damages.
Zhou
J F
JF
2nd Affiliated Hospital, Medical College of Zhejiang University, 88 Jiefang Road, Hangzhou, 310009, Zhejiang Province, China.
Chen
P
P
Yang
J L
JL
Zhu
Y G
YG
Peng
C H
CH
Wu
Y L
YL
D000328:Adult; D000368:Aged; D000975:Antioxidants / Q000032:analysis; D002764:Cholecystitis / Q000097:blood / Q000150:complications*; D002769:Cholelithiasis / Q000097:blood / Q000150:complications* / Q000601:surgery; D004912:Erythrocytes / Q000378:metabolism; D005260:Female; D006801:Humans; D008054:Lipid Peroxides / Q000032:analysis / Q000097:blood; D008297:Male; D008875:Middle Aged; D009569:Nitric Oxide / Q000032:analysis / Q000097:blood; D018384:Oxidative Stress; D011184:Postoperative Period; D011300:Preoperative Care; D012044:Regression Analysis; D014810:Vitamin E / Q000032:analysis / Q000097:blood
D000975:Antioxidants; D008054:Lipid Peroxides; D014810:Vitamin E; D009569:Nitric Oxide