FIA of vitamin C in blood serum in humans at increasing ethanol concentration.
The evaluation of the ethanol influence on the changes in vitamin C levels has been done in humans at increasing ethanol concentration. Flow injection analysis method (FIA) with the spectrophotometric detection was used to determine L-ascorbic acid levels. The procedure is based on the oxidation of the analysis with iron (III) and 2,2'-dipyrydyl. Concentrations of vitamin C in the range of 0.4-1.3 ppm have been determined with a relative standard deviation (R.S.D.) 1.2% (n=15). The injection rate was 40 samples h(-1). The comparison of L-ascorbic acid levels in humans showed a statistical difference at P<0.05 in the analysed group.
Kleszczewski
T
T
Institute of Biophysics, Medical Academy, Mickiewicza 2, 15-230, Bialystok, Poland. kleszczt@cksr.ac.bialystok.pl
Kleszczewska
E
E
D001205:Ascorbic Acid / Q000097:blood*; D000431:Ethanol / Q000494:pharmacology*; D017022:Flow Injection Analysis; D006801:Humans
D000431:Ethanol; D001205:Ascorbic Acid