Role of lipid transfer particle in delivery of diacylglycerol from midgut to lipophorin in larval Manduca sexta.
The present work analyzed the function of lipid transfer particle (LTP) in the process of exporting diacylglycerol from larval Manduca sexta midgut cells to lipophorin. When midgut sacs, which had been prelabeled in vivo with [(3)H]oleic acid, were incubated in vitro with a lipophorin-containing medium, a significant amount of radiolabeled diacylglycerol was transferred to lipophorin. Negligible amounts of diacylglycerol were released into lipophorin-free medium. In contrast, lipid-labeled lipophorin did not transfer diacylglycerol to the midgut sacs. The transfer of diacylglycerol from the midgut sac to lipophorin was blocked by preincubation of midgut sacs with antibody against LTP. Diacylglycerol transfer was restored to control values by the addition of purified LTP to midgut sacs that had been treated with antibody against LTP. Under these conditions the amount of diacylglycerol transferred was a function of the LTP concentration. These are the first results showing that LTP is required to export diacylglycerol from the midgut to lipophorin.
Canavoso
L E
LE
Department of Biochemistry and Molecular Biophysics and Center for Insect Science, Biological Sciences West, The University of Arizona, Tucson, AZ 85721-0088, USA.
Wells
M A
MA
D000818:Animals; D001053:Apolipoproteins / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism*; D004064:Digestive System / Q000378:metabolism; D004075:Diglycerides / Q000378:metabolism*; D007814:Larva; D050356:Lipid Metabolism; D008074:Lipoproteins / Q000378:metabolism*; D018402:Manduca / Q000378:metabolism; D011817:Rabbits
D001053:Apolipoproteins; D002352:Carrier Proteins; D004075:Diglycerides; D008074:Lipoproteins; C032830:lipophorin