title: Isolation of the third component of complement and its derivative with anaphylatoxin-like activity from the plasma of the newt Cynops pyrrhogaster.
abstract: The third component of complement (C3) of a newt, Cynops pyrrhogaster, was purified using a fast protein liquid chromatography technique. The purified newt C3 consists of two polypeptide chains (the molecular masses of the alpha and beta-chains of C3 were 120,000 and 70,000, respectively) linked by disulfide bonds. The alpha-chain retained an internal thiolester bond that was cleaved with methylamine, and the N-terminal amino acid sequence of the alpha-chain was XVQLIDAKAGKAAKF. Digestion of newt C3 with trypsin yielded fragments that induced significant histamine release from newt peritoneal cells. These results indicate that newt C3 retains structural and functional properties shared with mammalian C3.
authors.affiliation: Department of Material Science, Faculty of Science, Ehime University, Matsuyama, Japan. yoji@dpc.ehime-u.ac.jp
authors.forename: Y
authors.initials: Y
authors.lastname: Shimazaki
authors.forename: K
authors.initials: K
authors.lastname: Maeyama
authors.forename: T
authors.initials: T
authors.lastname: Fujii
mesh_terms: D000595:Amino Acid Sequence; D000706:Anaphylatoxins / Q000276:immunology* / Q000302:isolation & purification; D000818:Animals; D015932:Complement C3c / Q000276:immunology* / Q000302:isolation & purification; D015180:Electrophoresis, Gel, Two-Dimensional; D004952:Esters; D005260:Female; D006636:Histamine Release; D006801:Humans; D015151:Immunoblotting / Q000379:methods; D008297:Male; D008969:Molecular Sequence Data; D012455:Salamandridae / Q000276:immunology*; D013438:Sulfhydryl Compounds
chemical_list: D000706:Anaphylatoxins; D004952:Esters; D013438:Sulfhydryl Compounds; D015932:Complement C3c