The mechanism of inhibition of DNA synthesis by HH07A, a derivative of hainanensine.
The synthesis of DNA, RNA and protein in L1210 cells was inhibited by HH07A in a dose-dependent and time-dependent way. HH07A showed much stronger inhibitory effect on [3H]-thymidine incorporation than that on [3H]-uridine or [3H]-tyrosine incorporation. The methods of shift of absorption spectrum, shift of fluorescence emission spectrum and excitation spectrum, and isotope technique were used to investigate the mechanism of action of HH07A on DNA synthesis. The results suggested that the action of HH07A on DNA synthesis was not probably due to direct damage to the DNA template, it might mainly act by interfering with the metabolism of DNA.
Ye
Y
Y
Institute of Materia Medica, CAMS & PUMC, Beijing 100050.
Xu
C
C
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D004273:DNA, Neoplasm / Q000096:biosynthesis* / Q000378:metabolism; D006248:Harringtonines / Q000494:pharmacology*; D007939:Leukemia L1210 / Q000378:metabolism* / Q000473:pathology; D008297:Male; D009691:Nucleic Acid Denaturation; D014407:Tumor Cells, Cultured
D000972:Antineoplastic Agents, Phytogenic; D004273:DNA, Neoplasm; C095422:HH07A; D006248:Harringtonines