title: Preparation, characterization and in vitro biocompatibility evaluation of poly(butylene terephthalate)/wollastonite composites.
abstract: The aim of the study was to prepare composites of poly(butylene terepthalate)/wollastonite (PBT/W), evaluate their properties and in vitro biocompatibility. Composites of PBT with wollastonite in two different proportions, viz. 70/30 (PW-30), 50/50 (PW-50) were prepared. The DSC studies indicate marginal changes in the melting behavior and enhanced crystallization in PBT/W composites. The mechanical properties of the composites such as tensile modulus shows remarkable improvement as a result of incorporation of wollastonite. SEM studies of fractured surfaces of impact samples showed no evidence of bonding between PBT and wollastonite. Water contact angle of PW30 and PW50 was 73.7 and 78.7, respectively. In vitro biocompatibility of PW-30 was evaluated as a representative composite. Direct cell contact test did not show deleterious effects on NIH3T3 fibroblast morphology and DNA integrity indicating its compatibility. Leach out products (LOP) of PW-30 were evaluated non-toxic as tested by MTT assay. Mouse peritoneal macrophages in contact with PW-30 showed comparable expression of CD 11b/18, CD45, CD14 and B7.2 to macrophages in contact with PTFE control indicating its non-activating nature. LOP did not induce proliferation of mouse splenic lymphocytes suggesting its immuno-tolerance. PW-30 also exhibited preliminary blood compatibility. These physical properties and biocompatibility of PBT/W composites show their suitability as potential biomaterials.
authors.affiliation: Tissue Engineering and Banking Laboratory, National Centre for Cell Science, Ganeshkhind, Pune, India.
authors.forename: M
authors.initials: M
authors.lastname: Risbud
authors.forename: D N
authors.initials: DN
authors.lastname: Saheb
authors.forename: J
authors.initials: J
authors.lastname: Jog
authors.forename: R
authors.initials: R
authors.lastname: Bhonde
mesh_terms: D016475:3T3 Cells; D000818:Animals; D018786:Bone Substitutes / Q000737:chemistry* / Q000494:pharmacology; D017610:Calcium Compounds / Q000737:chemistry* / Q000494:pharmacology; D002152:Calorimetry, Differential Scanning / Q000379:methods; D002470:Cell Survival / Q000187:drug effects*; D003460:Crystallization; D005614:Freeze Fracturing; D006461:Hemolysis / Q000187:drug effects*; D006801:Humans; D066298:In Vitro Techniques; D008213:Lymphocyte Activation / Q000187:drug effects*; D008422:Materials Testing / Q000379:methods; D051379:Mice; D008807:Mice, Inbred BALB C; D008855:Microscopy, Electron, Scanning; D010795:Phthalic Acids / Q000737:chemistry* / Q000494:pharmacology; D011090:Polyenes / Q000737:chemistry* / Q000494:pharmacology; D017640:Silicates / Q000737:chemistry* / Q000494:pharmacology; D013154:Spleen / Q000276:immunology; D013314:Stress, Mechanical; D013329:Structure-Activity Relationship; D013718:Tensile Strength; D013816:Thermodynamics
chemical_list: D018786:Bone Substitutes; D017610:Calcium Compounds; D010795:Phthalic Acids; D011090:Polyenes; D017640:Silicates; C008952:polybutene; C031293:calcium silicate