A DAF-1-binding protein BRA-1 is a negative regulator of DAF-7 TGF-beta signaling.
We have identified homologs of a human BMP receptor-associated molecule BRAM1 in Caenorhabditis elegans. One of them, BRA-1, has been found to bind DAF-1, the type I receptor in the DAF-7 transforming growth factor-beta pathway through the conserved C-terminal region. As analyzed using a BRA-1GFP (green fluorescent protein) fusion gene product, the bra-1 gene is expressed in amphid neurons such as ASK, ASI, and ASG, where daf-1 is also expressed. A loss-of-function mutation in bra-1 exhibits robust suppression of the Daf-c phenotype caused by the DAF-7 pathway mutations. We propose that BRA-1 represents a novel class of receptor-associated molecules that negatively regulate transforming growth factor-beta pathways.
Morita
K
K
Department of Developmental Biology, National Institute for Basic Biology, Okazaki 444-8585, Japan.
Shimizu
M
M
Shibuya
H
H
Ueno
N
N
D048868:Adaptor Proteins, Signal Transducing; D000595:Amino Acid Sequence; D000818:Animals; D019677:Artificial Gene Fusion; D001483:Base Sequence; D001665:Binding Sites; D019556:COS Cells; D017173:Caenorhabditis elegans / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D029742:Caenorhabditis elegans Proteins; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002522:Chlorocebus aethiops; D017124:Conserved Sequence; D018509:DNA, Helminth; D049452:Green Fluorescent Proteins; D015801:Helminth Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008164:Luminescent Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D009474:Neurons / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology*; D016212:Transforming Growth Factor beta / Q000378:metabolism*
D048868:Adaptor Proteins, Signal Transducing; C430459:BRA-1 protein, C elegans; D029742:Caenorhabditis elegans Proteins; D002352:Carrier Proteins; C064359:DAF-1 protein, C elegans; C102095:DAF-7 protein, C elegans; D018509:DNA, Helminth; D015801:Helminth Proteins; D008164:Luminescent Proteins; D011956:Receptors, Cell Surface; D016212:Transforming Growth Factor beta; D049452:Green Fluorescent Proteins