title: The neuron restrictive silencer factor can act as an activator for dynamin I gene promoter activity in neuronal cells.
abstract: The neuron restrictive silencer element (NRSE) has been identified in several neuronal genes and confers neuron specificity by silencing transcription in nonneuronal cells. We have previously reported that Sp1 and an NF-kappaB-like element (NE-1) are required for the promoter activity of mouse dynamin I gene. In the present study, we found that the upstream regulatory region of the dynamin I promoter has an NRSE-like sequence and showed that neuron restrictive silencer factor (NRSF) binds to this element in neuronal cells as well as in nonneuronal cells. We also showed that NRSF activates the promoter activity of dynamin I gene in neuronal cells. From the results in this study, we suggest that NRSE might be involved in the neuron restriction of dynamin I expression, and NRSF could act as an activator for promoter activity of dynamin I gene in neuronal cells.
authors.affiliation: Cell Biology Laboratory, Korea Research Institute of Bioscience and Biotechnology, Yusung, Taejon, 305-600, Korea.
authors.forename: J
authors.initials: J
authors.lastname: Yoo
authors.forename: M J
authors.initials: MJ
authors.lastname: Jeong
authors.forename: S S
authors.initials: SS
authors.lastname: Lee
authors.forename: K I
authors.initials: KI
authors.lastname: Lee
authors.forename: B M
authors.initials: BM
authors.lastname: Kwon
authors.forename: D S
authors.initials: DS
authors.lastname: Kim
authors.forename: Y M
authors.initials: YM
authors.lastname: Park
authors.forename: M Y
authors.initials: MY
authors.lastname: Han
mesh_terms: D000818:Animals; D001483:Base Sequence; D004247:DNA; D034284:Dynamin I; D034281:Dynamins; D020558:GTP Phosphohydrolases / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D009474:Neurons / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D012097:Repressor Proteins / Q000502:physiology*; D014157:Transcription Factors / Q000502:physiology*; D000099229:RE1-Silencing Transcription Factor
chemical_list: D004247:DNA; D034284:Dynamin I; D034281:Dynamins; D020558:GTP Phosphohydrolases; D012097:Repressor Proteins; D014157:Transcription Factors; D000099229:RE1-Silencing Transcription Factor