[Effect of Tripterine on mRNA expression of c-myc and platelet derived growth factor of vascular smooth muscle cell in rats].
OBJECTIVE
To observe the effect of Tripterine on mRNA expression of c-myc and platelet derived growth factor (PDGF) in vascular smooth muscle cell (VSMC) of rats.


METHODS
5-10th passage culture of VSMC was used. Tripterine and 20% fetal calf serum were added into the medium of cultured VSMC in concentration of 0.1, 0.2 and 0.3 mg/L after free-serum cultivation for 24 hours. The general RNA was isolated from VSMC at 6 and 12 hours after the drug addition for detection of mRNA expression of c-myc and PDGF respectively by dot blot hybridization. The cDNA probes were labeled by digoxin.


RESULTS
Tripterine inhibited the PDGF mRNA expression of VSMC and decreased c-myc mRNA expression in a dose-dependent manner, either vs. control.


CONCLUSIONS
Tripterine could inhibit mRNA expression of c-myc and PDGF in VSMC, therefore, it would inhibit overproliferation of VSMC.
Chen
X
X
Department of Cardiology, First Affiliated Hospital of Beijing Medical University, Beijing(100034).
Wang
L
L
Feng
M
M
D000818:Animals; D002478:Cells, Cultured; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000166:cytology*; D053978:Pentacyclic Triterpenes; D010982:Platelet-Derived Growth Factor / Q000096:biosynthesis* / Q000235:genetics; D016271:Proto-Oncogene Proteins c-myc / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D014315:Triterpenes / Q000494:pharmacology*
D053978:Pentacyclic Triterpenes; D010982:Platelet-Derived Growth Factor; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger; D014315:Triterpenes; C050414:celastrol