He-Ne laser irradiation protects B-lymphoblasts from UVA-induced DNA damage.
The effect of He-Ne laser (632.8 nm) pre-irradiation on UVA (343 nm)-induced DNA damage in the human B-lymphoblast cell line NC37 was investigated using the comet assay. He-Ne laser pre-irradiation was observed to result in a dose-dependent decrease in UVA-induced DNA damage. This effect was also found to be dependent on the incubation period between He-Ne laser pre-irradiation and the UVA exposure. Whereas the control cells with a higher DNA damage point to an initial ability of faster repair, both the control and the He-Ne laser pre-irradiated cells subsequently show the same rate of DNA repair. The results suggest that He-Ne laser irradiation protect the cells from UVA-induced DNA damage primarily through an influence on processes that prevent an initial DNA damage.
Dube
A
A
Biomedical Applications Section, Laser R & D, Block D, Center for Advanced Technology, Indore 452013, India. okdube@cat.ernet.in
Bock
C
C
Bauer
E
E
Kohli
R
R
Gupta
P K
PK
Greulich
K O
KO
D001402:B-Lymphocytes / Q000528:radiation effects*; D002460:Cell Line; D020552:Comet Assay; D004247:DNA / Q000528:radiation effects; D004249:DNA Damage; D003627:Data Interpretation, Statistical; D006801:Humans; D007834:Lasers; D011835:Radiation Protection / Q000379:methods; D015203:Reproducibility of Results; D014466:Ultraviolet Rays
D004247:DNA