title: Transplanted MHC class I-deficient nonobese diabetic mouse islets are protected from autoimmune injury in diabetic nonobese recipients.
abstract: The injury of transplanted islets may occur by both autoimmune and alloimmune processes directed against MHC targets. To examine the role of MHC class I in islet graft injury, we transplanted syngeneic and allogeneic beta2-microglobulin-deficient islets into diabetic nonobese diabetic (NOD) mice. Loss of graft function was observed within 14 days using allogeneic C57BL/6 and BALB/c MHC class I deficient as well as wild-type MHC class I-bearing NOD donor islets. However, islets isolated from MHC class I-deficient NOD mice (NOD-B2 m-/-) survived indefinitely when transplanted under the kidney capsule of diabetic NOD recipients. Transplanted NOD-B2 m-/- islets were surrounded by a nondestructive periinsular infiltrate that expressed interleukin-4 in addition to interferon-gamma. These studies demonstrate the primary role of MHC class I molecules in causing autoimmune destruction or recurrent diabetes in transplanted islets.
authors.affiliation: Department of Microbiology & Immunology, University of Western Ontario, London Health Sciences Centre and the John P. Robarts Research Institute, Canada.
authors.forename: S
authors.initials: S
authors.lastname: Prange
authors.forename: P
authors.initials: P
authors.lastname: Zucker
authors.forename: A M
authors.initials: AM
authors.lastname: Jevnikar
authors.forename: B
authors.initials: B
authors.lastname: Singh
mesh_terms: D000818:Animals; D001327:Autoimmune Diseases / Q000517:prevention & control*; D003921:Diabetes Mellitus, Experimental / Q000503:physiopathology / Q000601:surgery*; D003922:Diabetes Mellitus, Type 1 / Q000276:immunology*; D006085:Graft Survival; D015395:Histocompatibility Antigens Class I / Q000032:analysis*; D007371:Interferon-gamma / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D007515:Islets of Langerhans / Q000276:immunology* / Q000473:pathology / Q000503:physiopathology; D016381:Islets of Langerhans Transplantation / Q000276:immunology*; D051379:Mice; D016688:Mice, Inbred NOD / Q000276:immunology*; D013601:T-Lymphocytes / Q000378:metabolism / Q000473:pathology
chemical_list: D015395:Histocompatibility Antigens Class I; D015847:Interleukin-4; D007371:Interferon-gamma