Development of a serum-free medium for a human immortalized fibroblast cell line (KMST-6/TNF) producing tumor necrosis factor-alpha (TNF-alpha) and growth inhibitory effects of its conditioned medium on malignant cells in culture.
Oh
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Miyazaki
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Namba
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D002455:Cell Division / Q000187:drug effects; D002461:Cell Line, Transformed; D017077:Culture Media, Conditioned; D016895:Culture Media, Serum-Free; D019856:Ethanolamine / Q000494:pharmacology; D005347:Fibroblasts / Q000378:metabolism*; D015870:Gene Expression; D006131:Growth Inhibitors / Q000494:pharmacology; D006801:Humans; D009369:Neoplasms / Q000473:pathology*; D010461:Peptones / Q000494:pharmacology; D014162:Transfection; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis* / Q000235:genetics
D017077:Culture Media, Conditioned; D016895:Culture Media, Serum-Free; D006131:Growth Inhibitors; D010461:Peptones; D014409:Tumor Necrosis Factor-alpha; D019856:Ethanolamine