A comprehensive characterization of a group IB intron and its encoded maturase reveals that protein-assisted splicing requires an almost intact intron RNA.
The group I intron (AnCOB) of the mitochondrial apocytochrome b gene from Aspergillus nidulans encodes a bi-functional maturase protein that is also a DNA endonuclease. Although the AnCOB intron self-splices, the encoded maturase protein greatly facilitates splicing, in part, by stabilizing RNA tertiary structure. To determine their role in self-splicing and in protein-assisted splicing, several peripheral RNA sub-domains in the 313 nucleotide intron were deleted (P2, P9, P9.1) or truncated (P5ab, P6a). The sequence in two helices (P2 and P9) was also inverted. Except for P9, the deleted regions are not highly conserved among group I introns and are often dispensable for catalytic activity. Nevertheless, despite the very tight binding of AnCOB RNA to the maturase and the high activity of the bimolecular complex (the rate of 5' splice-site cleavage was >20 min(-1) with guanosine as the cofactor), the intron was surprisingly sensitive to these modifications. Several mutations inactivated splicing completely and virtually all impaired splicing to varying degrees. Mutants containing comparatively small deletions in various regions of the intron significantly decreased binding affinity (generally >10(4)-fold), indicating that none of the domains that remained constitutes the primary recognition site of the maturase. The data argue that tight binding requires tertiary interactions that can be maintained by only a relatively intact intron RNA, and that the binding mechanism of the maturase differs from those of two other well-characterized group I intron splicing factors, CYT-18 and Cpb2. A model is proposed in which the protein promotes widespread cooperative folding of an RNA lacking extensive initial tertiary structure.
Geese
W J
WJ
Department of Biology, Temple University, Philadelphia, PA 19122, USA.
Waring
R B
RB
D001059:Apoproteins / Q000235:genetics; D001233:Aspergillus nidulans / Q000235:genetics*; D001483:Base Sequence; D001667:Binding, Competitive; D003573:Cytochrome b Group / Q000235:genetics; D045303:Cytochromes b; D004706:Endodeoxyribonucleases / Q000235:genetics / Q000378:metabolism*; D006151:Guanosine / Q000235:genetics / Q000378:metabolism; D006868:Hydrolysis; D007438:Introns / Q000235:genetics*; D007700:Kinetics; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000235:genetics / Q000378:metabolism; D009690:Nucleic Acid Conformation; D011485:Protein Binding; D022821:RNA Splice Sites / Q000235:genetics; D012326:RNA Splicing / Q000235:genetics*; D020871:RNA Stability; D016337:RNA, Catalytic / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D012194:RNA-Directed DNA Polymerase / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins; D017384:Sequence Deletion / Q000235:genetics; D013379:Substrate Specificity
D001059:Apoproteins; D003573:Cytochrome b Group; D009097:Multienzyme Complexes; D022821:RNA Splice Sites; D016337:RNA, Catalytic; D016601:RNA-Binding Proteins; D029701:Saccharomyces cerevisiae Proteins; D006151:Guanosine; D045303:Cytochromes b; C066564:aI2 protein, S cerevisiae; D012194:RNA-Directed DNA Polymerase; D004706:Endodeoxyribonucleases