Analysis of increased leukocyte ratio induced by tumor necrosis factor-alpha using several inhibitors in the undisturbed microcirculation of the rat mesentery.
Increased leukocyte ratio induced by intraperitoneal injection of TNF-alpha was analyzed in undisturbed rat mesentery venules, a long-term experimental model. TNF-alpha induced a biphasic increase in PMNL ratio in the mesentery venules; in particular, there was a large increase in PMNL ratio 3 h after injection of TNF-alpha. Anti-P and anti-L-selectin compounds, fucoidin and dextran sulfate, inhibited the PMNL ratio by about 40%. Dexamethasone completely inhibited the increased ratio. These results suggest that all selectins may be involved in the increased ratio, i.e. rolling and adhesion reaction.
Yamaki
K
K
National Food Research Institute, Ministry of Agriculture, Forestry and Fisheries, 2-1-2 Kannondai, Tsukuba, Ibaraki 305-8642, Japan. kyamaki@nfri.affrc.go.jp
Takano-Ishikawa
Y
Y
Kobori
M
M
D000818:Animals; D002465:Cell Movement / Q000187:drug effects; D003907:Dexamethasone / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D007958:Leukocyte Count; D007962:Leukocytes / Q000187:drug effects* / Q000276:immunology* / Q000502:physiology; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000276:immunology; D008297:Male; D008833:Microcirculation / Q000187:drug effects / Q000276:immunology; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000494:pharmacology; D019087:Selectins / Q000187:drug effects; D013152:Splanchnic Circulation / Q000187:drug effects / Q000276:immunology; D014372:Tubercidin / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000008:administration & dosage / Q000494:pharmacology*
D011994:Recombinant Proteins; D019087:Selectins; D014409:Tumor Necrosis Factor-alpha; C018258:3-deazaadenosine; D003907:Dexamethasone; D014372:Tubercidin