title: Interleukin-1 gene polymorphism and periodontal status. A case-control study.
abstract: OBJECTIVE
  This case-control study examined polymorphisms at the interleukin-1 gene in relation to periodontal status, subgingival bacteria and systemic antibodies to periodontal microbiota.
  
  
  METHODS
  132 periodontitis patients were age- and gender-matched with 73 periodontally intact controls. Full-mouth clinical assessments of the periodontal tissues were performed. Subgingival plaque samples (2440 in total) were analyzed by genomic DNA probes, and serum IgG antibodies to periodontal microbiota were assessed by an immunoassay. Polymorphisms in the IL-1A gene at position +4845 and the IL-1B gene at position +3953 were studied by PCR. A composite positive genotype was defined as at least one rare (#2) allele present at each locus.
  
  
  RESULTS
  No skewed distribution of the composite genotype was observed between cases and controls (45.2% vs 41.7%). In cases, both the composite genotype and the number of #2 alleles were positively correlated with the severity of attachment loss. No relationship between genotype and subgingival microbial profiles was observed. Genotype positive patients revealed both overall lower serum antibody levels and specific titers against selected bacteria.
  
  
  CONCLUSIONS
  The composite genotype failed to distinguish between periodontitis patients and controls but correlated in patients with the severity of the disease and the antibody responses to periodontal microbiota.
authors.affiliation: Department of Oral Microbiology, Faculty of Odontology, Göteborg University, Sweden. pp192@columbia.edu
authors.forename: P N
authors.initials: PN
authors.lastname: Papapanou
authors.forename: A M
authors.initials: AM
authors.lastname: Neiderud
authors.forename: J
authors.initials: J
authors.lastname: Sandros
authors.forename: G
authors.initials: G
authors.lastname: Dahlén
mesh_terms: D000328:Adult; D000483:Alleles; D000907:Antibodies, Bacterial / Q000097:blood; D001419:Bacteria / Q000145:classification; D016022:Case-Control Studies; D002874:Chromosome Mapping; D004269:DNA, Bacterial / Q000032:analysis; D003773:Dental Plaque / Q000382:microbiology; D005260:Female; D005838:Genotype; D005884:Gingival Hemorrhage / Q000145:classification; D005889:Gingival Recession / Q000145:classification / Q000235:genetics; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007375:Interleukin-1 / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D017622:Periodontal Attachment Loss / Q000145:classification / Q000235:genetics; D010514:Periodontal Pocket / Q000145:classification / Q000235:genetics; D010518:Periodontitis / Q000145:classification / Q000235:genetics / Q000276:immunology*; D011110:Polymorphism, Genetic / Q000235:genetics*; D012044:Regression Analysis
chemical_list: D000907:Antibodies, Bacterial; D004269:DNA, Bacterial; D007074:Immunoglobulin G; D007375:Interleukin-1