title: Estrogenic and antiestrogenic effect of in vitro treatment of follicular cells with 2,3,7,8-tetrachlorodibenzo-p-dioxin.
abstract: OBJECTIVE
  Two types of follicular cells from preovulatory ovary were cultured in vitro separately and in co-culture to test difference in 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) action on particular cell types.
  
  
  METHODS
  The accumulation of TCDD in follicular wall was analysed using coupled capillary gas chromatography mass spectrometry. Whole preovulatory follicles were isolated from ovary and incubated with prolonged exposure to 0.1 nM TCDD or single exposure to 10 nM TCDD for four days. In the second part of experiments direct effects of TCDD on steroidogenesis were investigated in porcine theca cells (Tc) and granulosa cells (Gc) cultured alone and in co-culture (GT). The media were collected after four days for steroid analysis.
  
  
  RESULTS
  59.3% and 81.2% of TCDD added to the culture medium was accumulated after 0.1 and 10 nM, respectively. TCDD in a dose-dependent manner increased estradiol secretion with concomitant progesterone secretion by theca interna cells. On the other hand decrease of both progesterone and estradiol secretion by granulosa cells cultured alone and in co-culture with theca cells was noted.
  
  
  CONCLUSIONS
  Different cell-specific estrogenic or antiestrogenic effect of TCDD were found in ovarian follicles.
authors.affiliation: Chemistry Department, Cracow University of Technology, Krakow, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Grochowalski
authors.forename: R
authors.initials: R
authors.lastname: Chrzaszcz
authors.forename: R
authors.initials: R
authors.lastname: Pieklo
authors.forename: E L
authors.initials: EL
authors.lastname: Gregoraszczuk
mesh_terms: D000818:Animals; D002450:Cell Communication; D018929:Cell Culture Techniques; D003470:Culture Media; D004305:Dose-Response Relationship, Drug; D004785:Environmental Pollutants / Q000633:toxicity*; D004958:Estradiol / Q000378:metabolism*; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006107:Granulosa Cells / Q000187:drug effects / Q000502:physiology; D006801:Humans; D006080:Ovarian Follicle / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D000072317:Polychlorinated Dibenzodioxins / Q000633:toxicity*; D011374:Progesterone / Q000378:metabolism*; D013552:Swine; D013799:Theca Cells / Q000187:drug effects / Q000502:physiology
chemical_list: D003470:Culture Media; D004785:Environmental Pollutants; D000072317:Polychlorinated Dibenzodioxins; D011374:Progesterone; D004958:Estradiol