Prevention of colonic mucosal regrowth after seromuscular enterocystoplasty.
OBJECTIVE
To find answers about preventing the colonic mucosal regrowth after seromuscular enterocystoplasty and the dissection pane, we performed an experimental study in dogs. We also report our experience with mucosal regrowth and bladder function after augmentation colocystoplasty.


METHODS
This study comprised 10 adult healthy female mongrel dogs. We performed this animal experiment in 2 stages. At stage 1 we constructed a low capacity bladder. Stage 2 was performed 6 months after stage 1. At the end of detrusorectomy a 15 cm. segment of sigmoid colon was detubularized. The animals were randomly divided into 2 groups. In the 5 group 1 dogs de-epithelialization was performed with needle point cautery at a low cut setting, and the mucosa, muscularis mucosa and submucosa were resected, leaving the fibers of the muscularis externa exposed. In the 5 group 2 dogs de-epithelialization was performed by the peeling technique, in which the dissection plane was developed between the mucosa and muscularis mucosa, and the muscularis mucosa and submucosa remained on the colonic muscle. The de-epithelialized patch of bowel was shaped as a cup patch and anastomosed to the edges of the detrusor muscle over the bulging urothelium. The animals were sacrificed at 12 months of followup. All bladders were inspected macroscopically for regrowth of the colonic mucosa. Multiple sections of the augmentation patches were obtained and processed routinely for histological evaluation to determine what happened to the urothelium.


RESULTS
We noted good urodynamic results in all dogs and there was no statistical difference in the groups in regard to bladder capacity. Mucosal regrowth did not develop in either group.


CONCLUSIONS
When dissection is performed successfully whether or not the submucosa is removed, there is no observed regrowth or loss of bladder capacity in either technique.
Kïbar
Y
Y
Department of Urology, Gülhane Military Medical Academy, Ankara, Turkey.
Tahmaz
L
L
Ongürü
O
O
Dayanç
M
M
D000818:Animals; D003106:Colon / Q000502:physiology* / Q000601:surgery; D004210:Dissection; D004285:Dogs; D005260:Female; D007413:Intestinal Mucosa / Q000502:physiology* / Q000601:surgery; D023421:Models, Animal; D011184:Postoperative Period; D011897:Random Allocation; D012704:Serous Membrane / Q000601:surgery; D001743:Urinary Bladder / Q000601:surgery*; D014563:Urodynamics; D013520:Urologic Surgical Procedures