title: Crystallization and quaternary structure analysis of an Lrp-like regulatory protein from the hyperthermophile Pyrococcus furiosus.
abstract: The LrpA transcriptional regulator from Pyrococcus furiosus, a member of the leucine-responsive regulatory protein (Lrp) family, has been crystallized by the hanging-drop method of vapour diffusion using ammonium sulfate as the precipitant. The crystals belong to the tetragonal system and are in space group I4(1)22, with unit-cell parameters a = b = 104.5, c = 245.1 A. Consideration of the values of V(M) and possible packing of the molecules within the cell suggest that the asymmetric unit contains a dimer. Examination of the behaviour of the protein on gel-filtration columns and analysis of the self-rotation function suggests that the molecule is an octamer in solution at around pH 5. Determination of the structure of this protein will provide insights into the mechanisms responsible for DNA-protein recognition at high temperature and into how the regulatory properties of the Lrp family are modified by the presence or absence of effector molecules.
authors.affiliation: Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, Sheffield S10 2TN, England.
authors.forename: S E
authors.initials: SE
authors.lastname: Sedelnikova
authors.forename: S H
authors.initials: SH
authors.lastname: Smits
authors.forename: P M
authors.initials: PM
authors.lastname: Leonard
authors.forename: A B
authors.initials: AB
authors.lastname: Brinkman
authors.forename: J
authors.initials: J
authors.lastname: van der Oost
authors.forename: J B
authors.initials: JB
authors.lastname: Rafferty
authors.forename: D W
authors.initials: DW
authors.lastname: Rice
mesh_terms: D019843:Archaeal Proteins / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D004268:DNA-Binding Proteins / Q000737:chemistry*; D051756:Leucine-Responsive Regulatory Protein; D011487:Protein Conformation; D020836:Protein Structure, Quaternary; D020027:Pyrococcus furiosus / Q000737:chemistry*; D014157:Transcription Factors / Q000737:chemistry*
chemical_list: D019843:Archaeal Proteins; D004268:DNA-Binding Proteins; C418107:LrpA protein, Pyrococcus furiosus; D014157:Transcription Factors; D051756:Leucine-Responsive Regulatory Protein