Molecular cloning and characterization of a mu-class glutathione S-transferase from Clonorchis sinensis.
In biliary passages, Clonorchis sinensis causes epithelial hyperplasia and is assumed to promote carcinogenesis. Glutathione S-transferase (GST) is an antioxidant enzyme involved in phase II defense in trematodes. A clone (pcsGSTM1) encoding a GST was identified by screening a C. sinensis cDNA library with a PCR-synthesized cDNA probe. The predicted amino acid sequence encoded by pcsGSTM1 cDNA had a high degree of sequence identity and folding topology similar to the mu-class GSTs. The estimated molecular mass of the protein, 26 kDa, was consistent with an expression by pcsGSTM1 cDNA. The bacterially expressed recombinant csGSTM1 protein possessed an enzymatic GST activity and conjugated GSH to reactive carbonyls of lipid peroxidation. The recombinant csGSTM1 protein did not share antigenic epitope(s) with GSTs of Fasciola hepatica, Paragonimus westermani and Schistosoma japonicum. The csGSTM1 was identified to a mu-class GST in C. sinensis.
Hong
S J
SJ
Department of Parasitology and Institute of Medical Science, Chung-Ang University College of Medicine, Tongjak-gu, 156-756, Seoul, South Korea. hongsj@cau.ac.kr
Lee
J Y
JY
Lee
D H
DH
Sohn
W M
WM
Cho
S Y
SY
D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D015139:Blotting, Southern; D003001:Cloning, Molecular; D003004:Clonorchis sinensis / Q000201:enzymology* / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D018509:DNA, Helminth / Q000032:analysis; D005982:Glutathione Transferase / Q000737:chemistry / Q000235:genetics* / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D010802:Phylogeny; D011817:Rabbits; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D018076:DNA, Complementary; D018509:DNA, Helminth; D005982:Glutathione Transferase; D011994:Recombinant Proteins