The effect of resveratrol on the platelet secretory process induced by endotoxin and thrombin.
The effect of resveratrol (trans-3,4',5-trihydroxystilbene) on the release of adenine nucleotides and proteins from blood platelets activated by lipopolysaccharide (LPS), from Proteus mirabilis and by thrombin, were studied. Thrombin stimulated the release of adenine nucleotides from dense granules and proteins from alpha-granules. The LPS (0.3 microg/10(8) platelets, 5 min, 37 degrees C), like thrombin (2.5 U/10(8) platelets, 5 min, 37 degrees C) was found to cause a release of adenine nucleotides and proteins (p <0.05). Resveratrol (6.25-100 microg/ml, 30 min, 37 degrees C) had a different effect on the platelet release reaction caused by either LPS or thrombin. The results indicated that resveratrol inhibited, in dose-dependent manner, the secretory process (release of adenine nucleotides and proteins) induced by thrombin (p <0.05), but it significantly stimulated the liberation of proteins from blood platelets activated by LPS (p <0.05).
Olas
B
B
Department of General Biochemistry, University of Lódź, Poland.
Wachowicz
B
B
Szewczuk
J
J
Saluk-Juszczak
J
J
Kaca
W
W
D000227:Adenine Nucleotides / Q000097:blood / Q000378:metabolism; D000818:Animals; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D001798:Blood Proteins / Q000378:metabolism; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D006490:Hemostatics / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D011513:Proteus mirabilis / Q000737:chemistry; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*; D013552:Swine; D013917:Thrombin / Q000494:pharmacology*
D000227:Adenine Nucleotides; D001798:Blood Proteins; D006490:Hemostatics; D008070:Lipopolysaccharides; D010975:Platelet Aggregation Inhibitors; D013267:Stilbenes; D013917:Thrombin; D000077185:Resveratrol