Transforming growth factor and neutralizing antibodies in subglottic stenosis.
Transforming growth factor beta 1 (TGF-beta1), which is implicated in the pathogenesis of fibrotic diseases such as interstitial fibrosis, may be associated with subglottic stenosis. To study this hypothesis, we measured TGF-beta1 expression sequentially in 28 rats after posterior cricoid injury, using both standard immunohistochemistry and reverse transcriptase-polymerase chain reaction. In addition, an osmotic pump infused TGF-beta1 in 18 rats, normal saline solution in 9 rats, and neutralizing antibodies in 9 rats. Specimens were stained for fibronectin and procollagen at 1, 7, and 21 days and underwent optical density analysis. In the injured airway, TGF-beta1 expression peaked at 1 day and returned to baseline by 21 days. The TGF-beta1 infusion led to an increase in the expression of extracellular matrix proteins relative to controls. In contrast, neutralizing antibodies led to a decrease in extracellular matrix protein expression. These findings suggest that TGF-beta1 may possibly play a role in the pathogenesis of subglottic stenosis.
Dillard
D G
DG
Department of Otolaryngology, Atlanta Veterans Administration Medical Center and Emory University School of Medicine, Georgia, USA.
Gal
A A
AA
Roman-Rodriguez
J
J
White
S
S
Jacobs
I N
IN
D000818:Animals; D005353:Fibronectins / Q000378:metabolism; D007150:Immunohistochemistry; D007829:Laryngostenosis / Q000188:drug therapy* / Q000378:metabolism*; D008297:Male; D011347:Procollagen / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016212:Transforming Growth Factor beta / Q000276:immunology / Q000378:metabolism* / Q000494:pharmacology*; D014945:Wound Healing / Q000187:drug effects
D005353:Fibronectins; D011347:Procollagen; D016212:Transforming Growth Factor beta