Cloning of the Aspergillus niger pmrA gene, a homologue of yeast PMR1, and characterization of a pmrA null mutant.
The pmrA gene, a yeast PMR1 homologue, was isolated from Aspergillus niger. Sequence analysis of the pmrA cDNA and the genomic DNA revealed that two introns exist in the coding region, and that an open reading frame in the cDNA encodes a polypeptide of 1056 amino acids containing all the conserved regions present in P-type Ca2+-ATPases. The predicted pmrA protein exhibited a high degree of sequence similarity to the Pmr1 proteins from yeasts and mammalians (50-59% identity). The expression of the pmrA cDNA partially restored the growth defect of Yarrowia lipolytica pmr1 null mutant on EGTA-containing medium. This indicates that the A. niger pmrA gene encodes a functional homologue of the yeast P-type Ca2+-ATPase involved in the secretory pathway. An A. niger pmrA null mutant exhibited growth retardation on EGTA-containing medium and the growth defect was overcome by adding Ca2+ or Mn2+ into the medium. This suggests an involvement of the pmrA protein in Ca2+ and Mn2+ homeostasis in A. niger.
Yang
J
J
Department of Microbiology, Chungnam National University, Taejon, South Korea.
Kang
H A
HA
Ko
S M
SM
Chae
S K
SK
Ryu
D D
DD
Kim
J Y
JY
D018528:ATP-Binding Cassette Transporters / Q000235:genetics; D000595:Amino Acid Sequence; D001234:Aspergillus niger / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D001483:Base Sequence; D000252:Calcium-Transporting ATPases / Q000235:genetics* / Q000378:metabolism*; D003001:Cloning, Molecular; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D017353:Gene Deletion; D005816:Genetic Complementation Test; D018832:Molecular Chaperones; D008969:Molecular Sequence Data; D029701:Saccharomyces cerevisiae Proteins; D004718:Saccharomycetales / Q000235:genetics / Q000254:growth & development; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
D018528:ATP-Binding Cassette Transporters; D005656:Fungal Proteins; D018832:Molecular Chaperones; C101767:SSC1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; C433210:pmrA protein, Aspergillus niger; D000252:Calcium-Transporting ATPases