title: Biofilm formation by gram-negative bacteria on central venous catheter connectors: effect of conditioning films in a laboratory model.
abstract: Human blood components have been shown to enhance biofilm formation by gram-positive bacteria. We investigated the effect of human blood on biofilm formation on the inner lumen of needleless central venous catheter connectors by several gram-negative bacteria, specifically Enterobacter cloacae, Pseudomonas aeruginosa, and Pantoea agglomerans. Results suggest that a conditioning film of blood components promotes biofilm formation by these organisms in an in vitro system.
authors.affiliation: Biofilm Research Laboratory, Division of Healthcare Quality Promotion, National Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30333, USA. rmurga@cdc.gov
authors.forename: R
authors.initials: R
authors.lastname: Murga
authors.forename: J M
authors.initials: JM
authors.lastname: Miller
authors.forename: R M
authors.initials: RM
authors.lastname: Donlan
mesh_terms: D001422:Bacterial Adhesion; D018441:Biofilms / Q000254:growth & development*; D001769:Blood; D002405:Catheterization, Central Venous / Q000009:adverse effects / Q000295:instrumentation*; D002408:Catheters, Indwelling / Q000382:microbiology*; D015169:Colony Count, Microbial; D006090:Gram-Negative Bacteria / Q000502:physiology*; D006801:Humans; D008954:Models, Biological