Detection of Aspergillus species DNA by PCR in bronchoalveolar lavage fluid.
The usefulness of a nested PCR assay for detection of Aspergillus sp. DNA was evaluated in 177 bronchoalveolar lavage (BAL) fluid specimens. This test was accurate both to diagnose culture-negative BAL fluid specimens from patients with invasive pulmonary aspergillosis and to confirm culture-positive samples. However, it did not differentiate between infection and colonization.
Hayette
M P
MP
Service de Microbiologie Médicale, Tour de Pathologie B23, Université de Liège, B-4000 Liège, Belgium. mphayette@ulg.ac.be
Vaira
D
D
Susin
F
F
Boland
P
P
Christiaens
G
G
Melin
P
P
De Mol
P
P
D001228:Aspergillosis / Q000382:microbiology*; D001230:Aspergillus / Q000235:genetics / Q000302:isolation & purification*; D001992:Bronchoalveolar Lavage Fluid / Q000382:microbiology*; D004271:DNA, Fungal / Q000032:analysis; D006801:Humans; D008172:Lung Diseases, Fungal / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods*
D004271:DNA, Fungal