[Enhancement of GAP-43 mRNA expression in the rat medial vestibular nucleus by labyrinthectomy].
Vestibular compensation is the most extensively investigated model for neuroplasticity. Growth-associated protein (GAP-43) mRNA plays a significant role in nerve regeneration and synaptic remodeling. Using in situ hybridization with DIG (digoxigenin)-labeled GAP-43 cDNA probe, changes of GAP-43 mRNA expression in the medial vestibular nucleus in the labyrinthectomized rats at 5, 12, 20 and 30th day after operation were investigated. The results clearly demonstrated that labyrinthectomy increased GAP-43 mRNA expression to different extents and amplitudes in bilateral medial vestibular nuclei. This finding suggests that upregulation of GAP-43 mRNA expression is related to regenerative sprouting, synaptic remodeling and neuroplasticity in vestibular compensation.
Sun
J R
JR
Peking University, College of Life Sciences, Beijing 100871.
Yu
R Y
RY
Liu
W S
WS
Wang
Y F
YF
D000222:Adaptation, Physiological; D000818:Animals; D007758:Ear, Inner / Q000502:physiology / Q000601:surgery; D019922:GAP-43 Protein / Q000096:biosynthesis* / Q000235:genetics; D008297:Male; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D009435:Synaptic Transmission; D014726:Vestibular Nuclei / Q000502:physiology*
D019922:GAP-43 Protein; D012333:RNA, Messenger