Reduced skeletal muscle calpain-10 transcript level is due to a cumulative decrease in major isoforms.
The DNA polymorphism SNP-43 in the calpain-10 gene is associated with insulin resistance and reduced skeletal muscle transcript in Pima Indians. Alternative splicing generates transcript isoforms calpain-10a to -10h. We determined the contribution of calpain-10 mRNA isoforms to the decreased total skeletal muscle calpain-10 mRNA levels observed in the G/G homozygotes. The expression levels of the major isoforms, calpain-10a and -10f, were positively correlated with the total calpain-10 mRNA levels, indicating a cumulative effect.
Yang
X
X
Clinical Diabetes and Nutrition Section, National Institute of Diabetes and Digestive and Kidney Diseases/NIH, 4212 N. 16th Street, Phoenix, AZ 85016, USA.
Pratley
R E
RE
Baier
L J
LJ
Horikawa
Y
Y
Bell
G I
GI
Bogardus
C
C
Permana
P A
PA
D002154:Calpain / Q000235:genetics*; D005260:Female; D005838:Genotype; D006801:Humans; D007198:Indians, North American / Q000235:genetics; D007527:Isoenzymes / Q000235:genetics; D008297:Male; D018482:Muscle, Skeletal / Q000201:enzymology / Q000378:metabolism*; D011110:Polymorphism, Genetic; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
D007527:Isoenzymes; D012333:RNA, Messenger; D002154:Calpain; C415197:calpain 10