title: Development of reverse transcriptase PCR assays for detection of active human herpesvirus 6 infection.
abstract: We developed reverse transcriptase (RT) PCR assays for the detection of mRNA from three spliced genes of human herpesvirus 6 (HHV-6), the immediate-early genes U16/U17 and U89/U90 and the late gene U60/U66. Sequence analysis determined the splicing sites of these genes. The new assays may be instrumental in investigating the association between HHV-6 and disease.
authors.affiliation: Laboratory of Experimental Hematology, University of Antwerp, Antwerp University Hospital, Wilrijkstraat 10, B-2650 Edegem, Belgium.
authors.forename: G
authors.initials: G
authors.lastname: Van den Bosch
authors.forename: G
authors.initials: G
authors.lastname: Locatelli
authors.forename: L
authors.initials: L
authors.lastname: Geerts
authors.forename: G
authors.initials: G
authors.lastname: Fagà
authors.forename: M
authors.initials: M
authors.lastname: Ieven
authors.forename: H
authors.initials: H
authors.lastname: Goossens
authors.forename: D
authors.initials: D
authors.lastname: Bottiger
authors.forename: B
authors.initials: B
authors.lastname: Oberg
authors.forename: P
authors.initials: P
authors.lastname: Lusso
authors.forename: Z N
authors.initials: ZN
authors.lastname: Berneman
mesh_terms: D017781:Genes, Immediate-Early; D005814:Genes, Viral; D006566:Herpesviridae Infections / Q000175:diagnosis* / Q000821:virology; D015654:Herpesvirus 6, Human / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D017874:Immediate-Early Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D012326:RNA Splicing; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000235:genetics
chemical_list: D017874:Immediate-Early Proteins; D012333:RNA, Messenger; D014764:Viral Proteins