A 'cassette' RNase: site-selective cleavage of RNA by RNase S equipped with RNA-recognition segment.
RNase S is a unique protein comprising the non-covalent association of two components, the S-peptide and the S-protein. An RNA-recognition segment derived from the human immunodeficiency virus (HIV)-1 Rev protein was conjugated with the S-peptide to form a complex with the S-protein. The resulting RNase S bearing the RNA-recognition segment preferentially hydrolyzed a single position of the RNA stem-loop derived from the specific binding site for the Rev protein.
Futaki
S
S
Institute for Chemical Research, Kyoto University, Uji, Japan. futaki@scl.kyoto-u.ac.jp
Araki
M
M
Kiwada
T
T
Nakase
I
I
Sugiura
Y
Y
D000595:Amino Acid Sequence; D054303:HIV Reverse Transcriptase / Q000737:chemistry; D015497:HIV-1 / Q000737:chemistry; D006868:Hydrolysis; D008958:Models, Molecular; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000187:drug effects*; D012260:Ribonucleases / Q000494:pharmacology*; D013379:Substrate Specificity
D012367:RNA, Viral; D054303:HIV Reverse Transcriptase; D012260:Ribonucleases; C022542:ribonuclease S