Paramecium genome survey: a pilot project.
A consortium of laboratories undertook a pilot sequencing project to gain insight into the genome of Paramecium. Plasmid-end sequencing of DNA fragments from the somatic nucleus together with similarity searches identified 722 potential protein-coding genes. High gene density and uniform small intron size make random sequencing of somatic chromosomes a cost-effective strategy for gene discovery in this organism.
Dessen
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Service de Bioinformatique, UMS825 CNRS/SC13 INSERM, 7 rue Guy Môquet BP8, 94801 Villejuif Cedex, France.
Zagulski
M
M
Gromadka
R
R
Plattner
H
H
Kissmehl
R
R
Meyer
E
E
Bétermier
M
M
Schultz
J E
JE
Linder
J U
JU
Pearlman
R E
RE
Kung
C
C
Forney
J
J
Satir
B H
BH
Van Houten
J L
JL
Keller
A M
AM
Froissard
M
M
Sperling
L
L
Cohen
J
J
D000818:Animals; D018503:Genome, Protozoan; D006801:Humans; D010247:Paramecium / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D010865:Pilot Projects; D015800:Protozoan Proteins / Q000235:genetics
D015800:Protozoan Proteins