title: Changes of HSP72-expression in leukocytes are associated with adaptation to exercise under conditions of high environmental temperature.
abstract: Overexpression of the heat shock protein HSP72 provides thermotolerance. We asked if two consecutive endurance runs 1 week apart (CR1, CR2) and additional environmental heat stress affect HSP72-expression in leukocytes of nonheat-acclimated endurance athletes. Twelve subjects were allocated randomly into two groups. Group HH completed both runs at 28 degrees C ambient temperature, and group NH performed CR1 at 18 degrees C and CR2 at 28 degrees C. HSP72-expression was determined by flow cytometry and RT-PCR before and 0, 24, and 48 h after exercise. Additionally, post-exercise cells were exposed to in vitro heat shock (HS; 2 h, 42 degrees C). The prolonged, high HSP72 protein level after CR1 in HH compared with NH may reflect thermotolerance induced by endurance exercise at high ambient temperature. Adaptation of cardiocirculatory/thermoregulatory capacity after CR2 in HH went along with a more rapid down-regulation of HSP72 compared with CR1. HSP72 mRNA demonstrated temperature-related changes after exercise. The reduced HS response in vitro after CR2 may represent exercise-related adaptation mechanisms. HSP72 concentrations in leukocytes may indicate previous exercise- and temperature-related stress conditions and adaptation in immunocompetent cells.
authors.affiliation: Department of Transfusion Medicine, Department of Sports Medicine, University of Tuebingen, Tuebingen, Germany. elvira.fehrenbach@med.uni-tuebingen.de
authors.forename: E
authors.initials: E
authors.lastname: Fehrenbach
authors.forename: A M
authors.initials: AM
authors.lastname: Niess
authors.forename: R
authors.initials: R
authors.lastname: Veith
authors.forename: H H
authors.initials: HH
authors.lastname: Dickhuth
authors.forename: H
authors.initials: H
authors.lastname: Northoff
mesh_terms: D000222:Adaptation, Physiological / Q000502:physiology*; D001831:Body Temperature; D002478:Cells, Cultured; D004777:Environment; D015870:Gene Expression; D050884:HSP72 Heat-Shock Proteins; D006339:Heart Rate; D006360:Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics; D018869:Heat-Shock Response; D006361:Heating; D006801:Humans; D019344:Lactic Acid / Q000097:blood; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008297:Male; D010101:Oxygen Consumption; D012420:Running
chemical_list: D050884:HSP72 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D019344:Lactic Acid