Interleukin-10 expression is correlated with growth fraction in human non-small cell lung cancer xenografts.
We examined IL-10 and IL-10 receptor (IL-10R) gene expression in 44 xenografts of human non-small cell lung cancer (NSCLC) by reverse transcription-polymerase chain reaction (RT-PCR) assay with human-specific primers. IL-10 gene expression was detected in 10 (22.7%) of 44 xenografts (0/11 adenocarcinomas, 6/19 squamous cell carcinomas, 4/14 large cell carcinomas) and IL-10R expression in 8 (18.2%) xenografts. The IL-10-positive xenografts immunohistochemically showed significantly increased growth fractions of neoplastic cells (471.0 +/- 112.2) relative to IL-10-negative xenografts (208.4 +/- 96.3) (p=0.009, Mann-Whitney U test), and showed significantly increased vascular number (p=0.008, Mann-Whitney U test). These results suggested that IL-10 expression is correlated with growth features in human NSCLC.
Naruke
M
M
Department of Surgery, School of Medicine, Keio University, Tokyo 160-8582, Japan.
Abe
Y
Y
Hatanaka
H
H
Ohnishi
Y
Y
Yamazaki
H
H
Kijima
H
H
Nakamura
M
M
Ueyama
Y
Y
Kobayashi
K
K
D000818:Animals; D002289:Carcinoma, Non-Small-Cell Lung / Q000378:metabolism / Q000473:pathology*; D017931:DNA Primers / Q000737:chemistry; D015870:Gene Expression; D006133:Growth Substances / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D007124:Immunoenzyme Techniques; D016753:Interleukin-10 / Q000096:biosynthesis / Q000235:genetics*; D008175:Lung Neoplasms / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D009389:Neovascularization, Pathologic / Q000473:pathology; D012333:RNA, Messenger / Q000378:metabolism; D018123:Receptors, Interleukin / Q000096:biosynthesis / Q000235:genetics; D053701:Receptors, Interleukin-10; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014183:Transplantation, Heterologous
D017931:DNA Primers; D006133:Growth Substances; D012333:RNA, Messenger; D018123:Receptors, Interleukin; D053701:Receptors, Interleukin-10; D016753:Interleukin-10