[Study on the analysis of zinc 5-aminosalicylate and related compounds by high performance liquid chromatography].
In this paper, the separation and determination conditions of zinc 5-aminosalicylate (ZASA) and related compounds by high performance liquid chromatography(HPLC) have been studied systematically. The baseline separation was achieved at 20 degrees C on a CLC-ODS column(150 mm x 6.0 mm i.d., 10 microns) within 10 minutes by using 1% (V/V) HAc-MeOH(4:6) as mobile phase at a flow-rate of 1 mL/min. The RSDs of rention time within day and between days were less than 2% and 5% respectively. The injection volume was 20 microL, and relationships between the peak area with 280 nm detection and the analyte concentration were linear within certain ranges. This method was used for determination of ZASA, impurities and thermal decomposition compounds. The results showed that the impurities in the crude ZASA were 5-aminosalicylic acid and salicylic acid. The thermal decomposition compounds in acid medium were 5-aminosalicylic acid, 5-aminophenol and 5-benzeneazosalicylic acid.
Liu
H
H
Center of Analysis & Testing, Zhengzhou University, Zhengzhou, 450052.
Zhang
S
S
Yuan
Z
Z
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D004339:Drug Compounding; D019804:Mesalamine / Q000032:analysis*; D020156:Salicylic Acid / Q000032:analysis*; D015032:Zinc / Q000032:analysis*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D019804:Mesalamine; D015032:Zinc; D020156:Salicylic Acid