Fiber-optic fluorometer for microscale mapping of photosynthetic pigments in microbial communities.
Microscale fluorescence measurements were performed in photosynthetic biofilms at a spatial resolution of 100 to 200 microm with a new fiber-optic fluorometer which allowed four different excitation and emission wavelengths and was configured for measuring phycobiliproteins, chlorophylls, and bacteriochlorophylls. We present details of the measuring system and describe examples of applications in different microbial communities.
Thar
R
R
Marine Biological Laboratory, University of Copenhagen, DK-3000 Helsingør, Denmark.
Kühl
M
M
Holst
G
G
D001426:Bacterial Proteins / Q000032:analysis; D001429:Bacteriochlorophylls / Q000032:analysis; D018441:Biofilms; D002734:Chlorophyll / Q000032:analysis; D000460:Chlorophyta; D000458:Cyanobacteria; D017753:Ecosystem; D005336:Fiber Optic Technology; D005470:Fluorometry / Q000295:instrumentation*; D019015:Geologic Sediments / Q000382:microbiology; D045342:Light-Harvesting Protein Complexes; D008386:Marine Biology; D008829:Microbiology / Q000295:instrumentation*; D055100:Optical Fibers; D010788:Photosynthesis; D010940:Plant Proteins / Q000032:analysis
D001426:Bacterial Proteins; D001429:Bacteriochlorophylls; D045342:Light-Harvesting Protein Complexes; D010940:Plant Proteins; D002734:Chlorophyll