title: In situ transcription and splicing in the Balbiani ring 3 gene.
abstract: The Balbiani ring 3 (BR3) gene contains 38 introns, and more than half of them are co-transcriptionally excised. We have determined the in situ structure of the active BR3 gene by electron tomography. Each of the 20-25 nascent transcripts on the gene is present together with splicing factors and the RNA polymerase II in a nascent transcript and splicing complex, here called the NTS complex. The results indicate that extensive changes in overall shape, substructure and molecular mass take place repeatedly within an NTS complex as it moves along the gene. The volume and calculated mass of the NTS complexes show that, maximally, one complete spliceosome is assembled on the multi-intron transcript at any given time point. The structural data show that the spliceosome is not a structurally well-defined unit in situ and that the C-terminal domain of the elongating RNA polymerase II cannot carry spliceosomal components for all introns in the BR3 transcript. Our data indicate that spliceosomal factors are continuously added to and released from the NTS complexes during transcription elongation.
authors.affiliation: Department of Cell and Molecular Biology, Medical Nobel Institute, Karolinska Institute, SE-171 77 Stockholm, Sweden.
authors.forename: I
authors.initials: I
authors.lastname: Wetterberg
authors.forename: J
authors.initials: J
authors.lastname: Zhao
authors.forename: S
authors.initials: S
authors.lastname: Masich
authors.forename: L
authors.initials: L
authors.lastname: Wieslander
authors.forename: U
authors.initials: U
authors.lastname: Skoglund
mesh_terms: D000818:Animals; D002683:Chironomidae / Q000235:genetics; D017344:Genes, Insect; D019476:Insect Proteins / Q000235:genetics*; D009690:Nucleic Acid Conformation; D012319:RNA Polymerase II / Q000378:metabolism; D012326:RNA Splicing; D017413:Ribonucleoprotein, U2 Small Nuclear / Q000378:metabolism; D012471:Salivary Proteins and Peptides / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: C112569:BR3 protein, Chironomus tentans; D019476:Insect Proteins; D017413:Ribonucleoprotein, U2 Small Nuclear; D012471:Salivary Proteins and Peptides; D012319:RNA Polymerase II