title: Na+,K+-ATPase and Ca2+-ATPase activities in the cochlear lateral wall following surgical induction of hydrops.
abstract: Na+,K+-ATPase and Ca2+-ATPase activities have not been studied quantitatively in the cochlea affected by endolymphatic hydrops. The present study was designed to measure quantitatively the Na+,K+-ATPase and Ca2+-ATPase activities in the cochlear lateral wall and the threshold of auditory brainstem response (ABR) for guinea pigs in the early stages (=2 months) of experimentally induced endolymphatic hydrops. A significant negative association was demonstrated between Ca2+-ATPase activity and the change in ABR threshold for hydropic cochleae (P=0.014), but not for control cochleae (P=0.123), although no such significant association was revealed between Na+,K+-ATPase activity and any change in ABR threshold for both hydropic cochleae (P=0.751) and control cochleae (P=0.352). A significant increase in Ca2+-ATPase activity in the cochlear lateral wall was observed for the hydropic ear, in which normal ABR thresholds were maintained, as compared to the control ear. On the contrary, a mild decrease in Ca2+-ATPase activity in the cochlear lateral wall was observed for the hydropic ear, in which ABR thresholds increased significantly. The present findings suggest that alterations of Ca2+-ATPase activity in the cochlear lateral wall may implicate disturbed calcium-homeostasis in the inner ear, resulting in hearing dysfunction in the early stages of experimentally induced endolymphatic hydrops.
authors.affiliation: Department of Otorlaryngology, National Taiwan University Hospital, Taipei. cjhsu@ha.mc.ntu.edu.tw
authors.forename: C J
authors.initials: CJ
authors.lastname: Hsu
authors.forename: C T
authors.initials: CT
authors.lastname: Tan
authors.forename: W Y
authors.initials: WY
authors.lastname: Shau
authors.forename: Y S
authors.initials: YS
authors.lastname: Chen
authors.forename: T H
authors.initials: TH
authors.lastname: Yeh
authors.forename: S Y
authors.initials: SY
authors.lastname: Lin-Shiau
mesh_terms: D000818:Animals; D001309:Auditory Threshold; D000252:Calcium-Transporting ATPases / Q000378:metabolism*; D003051:Cochlea / Q000201:enzymology* / Q000503:physiopathology; D004487:Edema / Q000201:enzymology* / Q000503:physiopathology; D016057:Evoked Potentials, Auditory, Brain Stem; D006168:Guinea Pigs; D008297:Male; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*
chemical_list: D000252:Calcium-Transporting ATPases; D000254:Sodium-Potassium-Exchanging ATPase