Subunit dissociation and reassociation leads to preferential crystallization of haemoglobin Bart's (gamma4) from solutions of human embryonic haemoglobin Portland (zeta2gamma2) at low pH.
A variety of human haemoglobins (Hbs) are produced at different stages of human development, including three embryonic Hbs, foetal Hb and adult Hb. All are heterotetramers. During crystallization experiments on human embryonic Hb Portland (zeta(2)gamma(2)), it was discovered by crystallographic and biochemical analysis that the homotetramer Hb Bart's (gamma(4)) preferentially crystallizes from zeta(2)gamma(2) solutions below pH 5. This results from dissociation of Hb Portland into gamma(2) dimers and zeta monomers and has interesting implications for subunit interactions and tetramer stability in Hbs. It also makes possible a full crystallographic analysis of Hb Bart's, which is of considerable medical significance because of its presence in the red blood cells of millions of people worldwide who suffer from alpha-thalassaemia.
Kidd
R D
RD
School of Biological Sciences, University of Auckland, Private Bag 92019, Auckland, New Zealand.
Mathews
A
A
Baker
H M
HM
Brittain
T
T
Baker
E N
EN
D002851:Chromatography, High Pressure Liquid; D003460:Crystallization; D018360:Crystallography, X-Ray; D006455:Hemoglobins, Abnormal / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D011487:Protein Conformation; D012996:Solutions
D006455:Hemoglobins, Abnormal; D012996:Solutions; C030447:hemoglobin Portland; C030448:hemoglobin Bart's