A sensitive nonradioisotopic method for detecting polymerase chain reaction products and its application to the quantification of mRNA levels in brown adipose tissue.
Takahashi
Y
Y
Laboratory of Nutrition Biochemistry, National Food Research Institute, Ministry of Agriculture, Forestry, and Fisheries, 2-1-2 Kannondai, Tsukuba Science City, Ibaraki 305-8642, Japan.
Ide
T
T
D002001:Adipose Tissue, Brown / Q000737:chemistry*; D000818:Animals; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D017931:DNA Primers / Q000737:chemistry; D018752:Diet, Fat-Restricted; D015870:Gene Expression; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics* / Q000378:metabolism; D007473:Ion Channels; D008163:Luminescent Measurements; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D024101:Mitochondrial Proteins; D016133:Polymerase Chain Reaction / Q000379:methods*; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D000071256:Uncoupling Protein 1
D002352:Carrier Proteins; D017931:DNA Primers; D007473:Ion Channels; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; D012333:RNA, Messenger; D000071256:Uncoupling Protein 1; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases