Murine germ cells do not require functional androgen receptors to complete spermatogenesis following spermatogonial stem cell transplantation.
The spermatogonial stem cell transplantation technique was employed to determine if murine germ cells require functional androgen receptors to complete qualitatively normal spermatogenesis. Testicular cells from testicular feminized mice were injected into the seminiferous tubules of azoospermic mice expressing functional androgen receptors. Recipient testes were analyzed between 110 and 200 days following transplantation. Multiple colonies of complete and qualitatively normal donor-derived spermatogenesis were seen within the seminiferous tubules of each recipient testis, demonstrating that murine germ cells do not require functional androgen receptors to complete spermatogenesis.
Johnston
D S
DS
School of Molecular Biosciences and Center for Reproductive Biology, Washington State University, Pullman 99164, USA.
Russell
L D
LD
Friel
P J
PJ
Griswold
M D
MD
D000818:Animals; D002454:Cell Differentiation; D005260:Female; D017353:Gene Deletion; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009154:Mutation; D009845:Oligospermia / Q000235:genetics; D016133:Polymerase Chain Reaction; D011944:Receptors, Androgen / Q000235:genetics / Q000502:physiology*; D012671:Seminiferous Tubules / Q000166:cytology; D013091:Spermatogenesis; D013093:Spermatogonia / Q000637:transplantation*; D013094:Spermatozoa / Q000502:physiology* / Q000648:ultrastructure; D033581:Stem Cell Transplantation; D013737:Testis / Q000166:cytology
D011944:Receptors, Androgen