Characterization of four members of a multigene family encoding outer membrane proteins of Helicobacter pylori and their potential for vaccination.
In search of protective antigens which can be used in a vaccine to prevent Helicobacter pylori infection, we report on the identification of four genes, hopV, hopW, hopX and hopY, and the characterization of the corresponding proteins which belong to the H. pylori outer membrane protein (Hop) family containing 32 homologous members, some of which were shown to function as porins. Sequence analysis of 16 different H. pylori strains revealed that the proteins HopV, HopW, HopX and HopY are highly conserved. Localization of HopV, HopW, HopX and HopY at the surface of the bacteria was investigated by immunofluorescence. Using a planar lipid bilayer system the proteins HopV and HopX were shown to form pores with single-channel conductances of 1.4 and 3.0 nS, respectively.
Peck
B
B
Chiron Behring GmbH & Co, Preclinical Research, D-35006 Marburg, Germany.
Ortkamp
M
M
Nau
U
U
Niederweis
M
M
Hundt
E
E
Knapp
B
B
D000595:Amino Acid Sequence; D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D015153:Blotting, Western; D005455:Fluorescent Antibody Technique; D016480:Helicobacter pylori / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D007106:Immune Sera; D008969:Molecular Sequence Data; D018272:Porins / Q000737:chemistry / Q000276:immunology; D011817:Rabbits; D011994:Recombinant Proteins / Q000276:immunology; D016415:Sequence Alignment
D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D007106:Immune Sera; D018272:Porins; D011994:Recombinant Proteins; C110353:hor protein, bacteria