Immunohistochemical analysis of NY-ESO-1 antigen expression in normal and malignant human tissues.
NY-ESO-1, a member of the CT (cancer/testis) family of antigens, is expressed in normal testis and in a range of human tumor types. Knowledge of NY-ESO-1 expression has depended on RT-PCR detection of mRNA and there is a need for detecting NY-ESO-1 at the protein level. In the present study, a method for the immunochemical detection of NY-ESO-1 in paraffin-embedded tissues has been developed and used to define the expression pattern of NY-ESO-1 in normal tissues and in a panel of human tumors. No normal tissue other than testis showed NY-ESO-1 reactivity, and expression in testis was restricted to germ cells particularly spermatogonia. In human tumors, the frequency of NY-ESO-1 antigen expression corresponds with past analysis of NY-ESO-1 mRNA expression e.g., 20-30% of lung cancers, bladder cancers and melanoma, and no expression in colon and renal cancer. Co-typing of NY-ESO-1 antigen and mRNA expression in a large panel of lung cancers showed a good correlation. There is great variability in NY-ESO-1 expression in individual tumors, ranging from an infrequent homogeneous pattern of staining to highly heterogeneous antigen expression.
Jungbluth
A A
AA
Ludwig Institute for Cancer Research, Memorial Sloan-Kettering Cancer Center, New York, NY, USA. jungblua@mskcc.org
Chen
Y T
YT
Stockert
E
E
Busam
K J
KJ
Kolb
D
D
Iversen
K
K
Coplan
K
K
Williamson
B
B
Altorki
N
N
Old
L J
LJ
D000911:Antibodies, Monoclonal / Q000378:metabolism; D000951:Antigens, Neoplasm; D001943:Breast Neoplasms / Q000378:metabolism; D002292:Carcinoma, Renal Cell / Q000378:metabolism; D003110:Colonic Neoplasms / Q000378:metabolism; D005260:Female; D006258:Head and Neck Neoplasms / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D007680:Kidney Neoplasms / Q000378:metabolism; D008175:Lung Neoplasms / Q000378:metabolism; D008297:Male; D008545:Melanoma / Q000378:metabolism; D008565:Membrane Proteins; D014176:Protein Biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013093:Spermatogonia / Q000378:metabolism; D013737:Testis / Q000378:metabolism*; D014018:Tissue Distribution; D014407:Tumor Cells, Cultured; D001749:Urinary Bladder Neoplasms / Q000378:metabolism
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; C104948:CTAG1B protein, human; D008565:Membrane Proteins