Tailor-made dyes for fluorescence correlation spectroscopy (FCS).
Two new fluorescent labels are presented that are optimized for excitation with He/Ne laser and red diode lasers. Application in FCS and labeling of proteins and oligomers are demonstrated. A strong rise of quantum yield and emission life time upon binding to biomolecules are characteristic features of the dyes.
Czerney
P
P
Dyomics GmbH, Bioinstrumentezentrum, Jena, Germany.
Lehmann
F
F
Wenzel
M
M
Buschmann
V
V
Dietrich
A
A
Mohr
G J
GJ
D001578:Benzopyrans / Q000737:chemistry*; D004247:DNA / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D007211:Indoles / Q000737:chemistry*; D007834:Lasers; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013329:Structure-Activity Relationship
D001578:Benzopyrans; C439044:DY 630; C439045:DY 635; D005456:Fluorescent Dyes; D007211:Indoles; D012710:Serum Albumin, Bovine; D004247:DNA