Differential regulation of survival and growth in adult sympathetic neurons: an in vitro study of neurotrophin responsiveness.
The survival and growth of embryonic and postnatal sympathetic neurons is dependent on both NGF and NT3. While it has been established that adult sensory neurons survive independently of neurotrophins, the case is less clear for adult sympathetic neurons, where the studies of survival responses to neurotrophins have relied upon using long-term cultures of embryonic neurons. We have previously established a method to culture purified young (7 day) and adult (12 week) sympathetic neurons isolated from adult rat superior cervical ganglia (SCG) in order to examine their survival and growth responses to neurotrophins. We now show that by 12 weeks after birth virtually all neurons (90%) survive for 24 h in the absence of neurotrophins. Neuron survival is unaffected by treatment with anti-NGF antibodies (anti-NGF) or with the tyrosine kinase inhibitor, K252a, confirming the lack of dependence on extrinsic neurotrophins. Duration of neuron survival in culture increases significantly between E19 and day 7 and week 12 posnatally, and is similarly unaffected by the presence of anti-NGF or K252a. Saturating concentrations of NGF and NT3 are equipotent in promoting neurite extension and branching. However, we find that NGF is more potent than NT3 in promoting neurite growth, irrespective of postnatal age. The growth-promoting effects of NGF and NT3 are almost entirely blocked by K252a, demonstrating that these effects are mediated via activation of Trk receptors, which therefore appear to remain crucial to plasticity of adult neurons. Our results indicate that maturing neurons acquire protection against cell death, induced in the absence of neurotrophin, while retaining their growth responsiveness to these factors.
Orike
N
N
Department of Anatomy and Developmental Biology, Royal Free and University College Medical School, Royal Free Campus, London, United Kingdom.
Thrasivoulou
C
C
Wrigley
A
A
Cowen
T
T
D000818:Animals; D000831:Animals, Newborn / Q000502:physiology*; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D029721:Drosophila Proteins; D004622:Embryo, Mammalian / Q000502:physiology; D005314:Embryonic and Fetal Development; D019476:Insect Proteins / Q000502:physiology; D008297:Male; D020932:Nerve Growth Factor / Q000494:pharmacology / Q000502:physiology*; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D020933:Neurotrophin 3 / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction; D017783:Superior Cervical Ganglion / Q000166:cytology* / Q000196:embryology*; D013997:Time Factors
D029721:Drosophila Proteins; D019476:Insect Proteins; D020933:Neurotrophin 3; C096356:trk protein, Drosophila; D020932:Nerve Growth Factor