title: Renaut bodies in the sciatic nerve of beagle dogs.
abstract: 182 control Beagle dogs from 23 historical studies (14 chronic, 9 subchronic) were reviewed histologically for the presence of Renaut bodies in the sciatic nerve. Renaut bodies were found in 36.1 percent of the subchronic-study dogs and in 46.4 percent of the chronic-study dogs. The Renaut bodies most often resided in the distal sections of the sciatic nerve, specifically in the tibial branch as it traversed the knee joint in situ. There was no sex predilection. Renaut bodies were located predominately in the endoneurium, in the center of the nerve sections. There was no associated axonal degeneration, reactive gliosis, or encapsulation. The Renaut bodies were characterized as large (20 to 500 microns diameter in cross section), well-demarcated elliptical structures with an onion-skin arrangement of loosely textured, filamentous strands intermixed with sparse numbers of dark spindle-shaped nuclei. Occasionally the core displayed a more dense, intensely eosinophilic arrangement of fibers. Histochemical results included: positive acidic alcian blue, Gomori's trichrome, and Verhoeff Van Gieson's; and negative Periodic-acid Schiff, Congo Red, and Luxol fast blue/cresyl violet. Immunohistochemical results included: positive vimentin and collagen (subtypes I, II, and VI); and negative NSE, S-100, GFAP, amyloid A component, desmin, alpha-sarcomeric actin, pancytokeratin, EMA, and von Willebrand factor. Transmission electron microscopy revealed loosely arrayed, circumferentially oriented collagen fibers intermixed with varying amounts of amorphous substance and finely fibrillar material. Most of the cells comprising the Renaut body were identified as fibroblasts. No nerve fibers entered or left the Renaut body, and nearby nerves appeared to be normal structurally. Based on this characterization of Renaut bodies and in conjunction with the past literature, Renaut bodies appear to have little or no pathological significance, but rather are suggestive of a physiological adaptation in response to mechanical stress imposed on nerves.
authors.affiliation: Bayer Corporation, Toxicology Department, Stilwell, Kansas 66085, USA. laura.elock.b@bayer.com
authors.forename: L E
authors.initials: LE
authors.lastname: Elcock
authors.forename: B P
authors.initials: BP
authors.lastname: Stuart
authors.forename: H E
authors.initials: HE
authors.lastname: Hoss
authors.forename: K
authors.initials: K
authors.lastname: Crabb
authors.forename: D M
authors.initials: DM
authors.lastname: Millard
authors.lastname: Bopp BILL
authors.forename: R E
authors.initials: RE
authors.lastname: Mueller
authors.forename: T F
authors.initials: TF
authors.lastname: Hastings
authors.forename: S G
authors.initials: SG
authors.lastname: Lake
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis; D004285:Dogs; D018604:Epitope Mapping; D005260:Female; D007124:Immunoenzyme Techniques; D008297:Male; D008854:Microscopy, Electron; D009940:Organoids / Q000737:chemistry / Q000648:ultrastructure*; D010525:Peripheral Nerves / Q000648:ultrastructure; D012584:Sciatic Nerve / Q000737:chemistry / Q000166:cytology*; D013194:Staining and Labeling
chemical_list: D015415:Biomarkers