title: Effects of medium perfusion on matrix production by bovine chondrocytes in three-dimensional collagen sponges.
abstract: Various culture systems have been used for examining the anabolic and catabolic functions of isolated chondrocytes as well as for tissue engineering purposes. Perfusion or frequent medium change is beneficial for three-dimensional (3D) cultures of many cell types. In this study, bovine articular chondrocytes (bACs) were grown in 3D collagen sponges with or without medium perfusion (0.33 mL/min) for up to 15 days. The influence of medium perfusion was evaluated using markers of cartilage matrix accumulation, synthesis, and gene expression. Metachromatic matrix, collagen type II, and hyaluronan accumulated around the cells within the collagen sponges. Sulfated glycosaminoglycans (S-GAGs) that accumulated in the sponge exposed to nonperfused control were 130% of that in the perfused sponge at day 7. S-GAG accumulation after 15 days in the nonperfused control was 230% more than at day 7 (p < 0.01). (35)S-sulfate incorporation during the final 18 h of culture in the sponge exposed to nonperfusion was 180% greater than that in the perfused sponge (p < 0.01). Quantitative analyses show that at day 7, aggrecan and collagen type II gene expression were 350% and 240% greater, respectively, in the nonperfused culture than in the perfused one. These results indicate that perfused conditions that are beneficial for other cell types inhibit chondrogenesis by articular chondrocytes in 3D culture.
authors.affiliation: Department of Orthopedic Surgery, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts, USA.
authors.forename: S
authors.initials: S
authors.lastname: Mizuno
authors.forename: F
authors.initials: F
authors.lastname: Allemann
authors.forename: J
authors.initials: J
authors.lastname: Glowacki
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D002417:Cattle; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000379:methods; D019902:Chondrocytes / Q000166:cytology* / Q000378:metabolism; D003094:Collagen; D003470:Culture Media; D005109:Extracellular Matrix / Q000378:metabolism; D010477:Perfusion
chemical_list: D001672:Biocompatible Materials; D003470:Culture Media; D003094:Collagen