The complete genome sequence of the murine respiratory pathogen Mycoplasma pulmonis.
Mycoplasma pulmonis is a wall-less eubacterium belonging to the Mollicutes (trivial name, mycoplasmas) and responsible for murine respiratory diseases. The genome of strain UAB CTIP is composed of a single circular 963 879 bp chromosome with a G + C content of 26.6 mol%, i.e. the lowest reported among bacteria, Ureaplasma urealyticum apart. This genome contains 782 putative coding sequences (CDSs) covering 91.4% of its length and a function could be assigned to 486 CDSs whilst 92 matched the gene sequences of hypothetical proteins, leaving 204 CDSs without significant database match. The genome contains a single set of rRNA genes and only 29 tRNAs genes. The replication origin oriC was localized by sequence analysis and by using the G + C skew method. Sequence polymorphisms within stretches of repeated nucleotides generate phase-variable protein antigens whilst a recombinase gene is likely to catalyse the site-specific DNA inversions in major M.pulmonis surface antigens. Furthermore, a hemolysin, secreted nucleases and a glyco-protease are predicted virulence factors. Surprisingly, several of the genes previously reported to be essential for a self-replicating minimal cell are missing in the M.pulmonis genome although this one is larger than the other mycoplasma genomes fully sequenced until now.
Chambaud
I
I
INRA-Université de Bordeaux 2, Institut de Biologie Végétale Moléculaire, 71 avenue Edouard Bourleaux, BP 81, 33883 Villenave D'Ornon Cedex, France.
Heilig
R
R
Ferris
S
S
Barbe
V
V
Samson
D
D
Galisson
F
F
Moszer
I
I
Dybvig
K
K
Wróblewski
H
H
Viari
A
A
Rocha
E P
EP
Blanchard
A
A
D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology; D001482:Base Composition; D018388:Codon, Terminator / Q000235:genetics; D019295:Computational Biology; D019143:Evolution, Molecular; D005815:Genetic Code; D016678:Genome; D015698:Genomic Library; D006801:Humans; D020407:Internet; D008074:Lipoproteins / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D009174:Mycoplasma / Q000235:genetics* / Q000276:immunology / Q000472:pathogenicity*; D016366:Open Reading Frames / Q000235:genetics; D011110:Polymorphism, Genetic / Q000235:genetics; D012329:RNA, Bacterial / Q000235:genetics; D011995:Recombination, Genetic / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D018741:Replication Origin / Q000235:genetics; D012137:Respiratory System / Q000382:microbiology*; D014774:Virulence / Q000235:genetics
D000942:Antigens, Bacterial; D018388:Codon, Terminator; D008074:Lipoproteins; D012329:RNA, Bacterial