Improvement of quantitative PCR reproducibility by betaine as determined by fluorescence-based method.
Shammas
F V
FV
Rogaland Central Hospital, Stavanger, Norway. shammas@online.no
Heikkilä
R
R
Osland
A
A
D001622:Betaine; D019457:Chromosome Breakage / Q000235:genetics; D002892:Chromosomes, Human, Pair 22; D018076:DNA, Complementary / Q000032:analysis; D004121:Dimethyl Sulfoxide; D004587:Electrophoresis, Agar Gel; D005456:Fluorescent Dyes; D006801:Humans; D007202:Indicators and Reagents; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D019914:Taq Polymerase
D018076:DNA, Complementary; D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D001622:Betaine; D019914:Taq Polymerase; D004121:Dimethyl Sulfoxide