title: Two functional O-polysaccharide polymerase wzy (rfc) genes are present in the rfb gene cluster of Group E1 Salmonella enterica serovar Anatum.
abstract: Defined regions of the rfb gene cluster of Group E1 Salmonella enterica serovar Anatum were introduced into a mutated derivative of this strain that lacks O-polysaccharide polymerase activity. Three different kinds of assays performed on the various transformants all indicate that two functional wzy (rfc) genes reside within the Group E1 Salmonella rfb gene cluster. The product of ORF9.6, positioned near the center of the rfb gene cluster, joins O-polysaccharide repeat units together by alpha-glycosidic linkages to produce antigen O10, the major serological determinant of Group E1 S. enterica. The product of ORF17.4, positioned at the downstream end of the rfb gene cluster, can join repeat units together by beta-glycosidic linkages to produce antigen O15, the major serological determinant of Group E2 S. enterica.
authors.affiliation: Department of Biology, Point Loma Nazarene University, 3900 Lomaland Drive, San Diego, CA 92106, USA. mmcconne@ptloma.edu
authors.forename: M R
authors.initials: MR
authors.lastname: McConnell
authors.forename: K R
authors.initials: KR
authors.lastname: Oakes
authors.forename: A N
authors.initials: AN
authors.lastname: Patrick
authors.forename: D M
authors.initials: DM
authors.lastname: Mills
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001435:Bacteriophages / Q000502:physiology; D004586:Electrophoresis; D004797:Enzyme-Linked Immunosorbent Assay; D016680:Genome, Bacterial; D006602:Hexosyltransferases / Q000235:genetics* / Q000378:metabolism; D008070:Lipopolysaccharides / Q000378:metabolism; D005810:Multigene Family; D019779:Salmonella enterica / Q000235:genetics* / Q000276:immunology / Q000378:metabolism / Q000821:virology; D012703:Serotyping
chemical_list: D001426:Bacterial Proteins; D008070:Lipopolysaccharides; C079397:rfb protein, Bacteria; D006602:Hexosyltransferases; C085652:O-antigen polymerase