Expression of three vitelline envelope protein genes in arctic char.
Several studies have shown effects of estrogenic substances on endocrine and reproductive systems in wildlife. Measurement of plasma vitellogenin (VTG) is a commonly used method to determine exposure to estrogenic substances in fish. There is, however, a growing need for additional sensitive and accurate methods to detect estrogenic substances in vivo. The vitelline envelope proteins (VEPs) have been suggested, in other studies, as suitable biomarkers for estrogenic substances. The present study investigates the induction of VEPs in juvenile Arctic char (Salvelinus alpinus). The results demonstrate that VEP mRNA exhibits earlier induction than estrogen receptor mRNA or VTG mRNA following injection of juvenile Arctic char with a single dose of 17beta-estradiol (E2; 10 mg/kg bw). These results indicate that the VEPs have a higher sensitivity for E2 than VTG. However, an early and sex-independent expression of VEPbeta in estrogen-unchallenged juvenile Arctic char was observed. These findings suggests that the regulatory mechanisms of VEPs might be more complex than previously thought, which in turn may have implications for the usage of VEPs as biomarkers for xenoestrogen exposure.
Westerlund
L
L
Department of Cell and Molecular Biology, Umeå University, Umeå, SE-901 87, Sweden.
Hyllner
S J
SJ
Schopen
A
A
Olsson
P E
PE
D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D004527:Egg Proteins / Q000097:blood / Q000235:genetics*; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D015870:Gene Expression / Q000187:drug effects*; D008297:Male; D012333:RNA, Messenger / Q000097:blood; D011960:Receptors, Estrogen / Q000235:genetics; D012727:Sex Characteristics; D012741:Sexual Maturation; D014337:Trout / Q000378:metabolism*; D014819:Vitellogenins / Q000097:blood / Q000235:genetics
D004527:Egg Proteins; D012333:RNA, Messenger; D011960:Receptors, Estrogen; D014819:Vitellogenins; C035651:vitelline membrane proteins; D004958:Estradiol