beta-Elimination of glucosyluronic residues during methylation of an acidic polysaccharide from Erwinia chrysanthemi CU 643.
The Erwinia chrysanthemi CU643 EPS has a linear hexasaccharide repeating unit in which a 4-linked uronic acid residue is present. The EPS was methylated by either the NaOH-Me2SO-MeI or Li-dimsyl procedure. MALDI-TOF MS analysis of the methylated products indicates that the beta-eliminative degradation occurs during the methylation, as characterized by serial fragments of the hexasaccharide repeating units. The degradation was clearly defined from the methylation of a glucosyluronic-containing pyruvated pentasaccharide.
Yun Yang
B
B
Department of Biochemistry, College of Medicine, University of Iowa, Iowa City, Iowa, IA 52242, USA.
Montgomery
R
R
D016974:Dickeya chrysanthemi / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D005965:Glucuronates / Q000378:metabolism*; D008745:Methylation; D009844:Oligosaccharides / Q000378:metabolism; D011134:Polysaccharides / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D005965:Glucuronates; D009844:Oligosaccharides; D011134:Polysaccharides