Reduction in mitochondrial respiratory capacity in Saccharomyces cerevisiae induced by expression of hepatitis B virus surface antigen.
Transformants of Saccharomyces cerevisiae strain TL154 (MATalpha, trp1, leu2) expressing hepatitis B virus surface antigen showed reduced rates of cell growth compared with those of nontransformed cells. The rates of phosphorylative, nonphosphorylative, and uncoupled respiration in mitochondria isolated from the transformants were reduced relative to those of mitochondria derived from nontransformed cells, regardless of whether the cells were cultured in rich or minimal medium. The electrophoretic protein profiles of cell and mitochondrial extracts did not differ substantially between transformed and nontransformed cells. These results suggest that the reduced rate of mitochondrial respiration in the transformants may be due to impairment of metabolic function rather than to inhibition of the expression of components of the respiratory chain.
Chien
L F
LF
Institute of Molecular Biology, Academia Sinica, Taiwan, Republic of China.
Kuo
T T
TT
D003470:Culture Media; D015966:Gene Expression Regulation, Fungal; D006514:Hepatitis B Surface Antigens / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D007700:Kinetics; D008928:Mitochondria / Q000378:metabolism*; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development / Q000276:immunology* / Q000378:metabolism; D014170:Transformation, Genetic
D003470:Culture Media; D006514:Hepatitis B Surface Antigens