[Effect of a culturing method and growth phase on composition of lipopolysaccharides in Yersinia pseudotuberculosis].
The effects of the culturing method (suspension cultures in a liquid nutrient broth or colonies on a solid agarized medium) and the growth phase on the lypopolysaccharide (LPS) composition of Yersinia pseudotuberculosis (O:Ib serovar, strain KS 3058) grown in cold (5 degrees C) were studied. The amount the LPS synthesized by cells depended on the bacteria growth phase for both media. The LPS acylation degree was constant, whereas the length of the O-specific polysaccharide chain varied with the culture age and achieved maximum in the stationary growth phase for both media. The bacteria culturing on the nutrient agar stimulated more intensive synthesis of LPS, which were extracted more easily, had longer polysaccharide O-chains, and were more toxic than LPS of the bacteria cultured in the liquid medium. It was proposed that the culturing of Yersinia pseudotuberculosis in cold as colonies on the agar surface causes an increase in the bacterial virulence.
Bakholdina
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SI
Pacific Institute of Bioorganic Chemistry, Far East Division, Russian Academy of Sciences, pr. 100-letiya Vladivostoka 159, Vladivostok, 690022 Russia. piboc@stl.ru
Krasikova
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IN
Solov'eva
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TF
D000362:Agar; D000818:Animals; D002455:Cell Division; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D007928:Lethal Dose 50; D008070:Lipopolysaccharides / Q000096:biosynthesis / Q000737:chemistry* / Q000633:toxicity; D051379:Mice; D015011:Yersinia pseudotuberculosis / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism
D003470:Culture Media; D008070:Lipopolysaccharides; D000362:Agar