[Effects of c-myc antisense oligodeoxynucleotide transfected by liposome on HL-60 cells].
OBJECTIVE
To investigate a delivery system for administering antisense oligodeoxynucleotide (AS-ODN) to HL-60 cells and study the antitumor effects of c-myc antisense oligodeoxynucleotide.


METHODS
Human leukemia cell line HL-60 was transfected with a synthetic oligodeoxynucleotide(ODN) complementary to the 5' end region of c-myc gene. In addition, the changes of function and morphology were observed after transfection of cationic liposome.


RESULTS
HL-60 cells incubated with c-myc AS-ODN/lipofectin complex were about 50% decreased in the 4th day as compared with control cells (P < 0.05). About 56% of HL-60 cells exposed to c-myc AS-ODN/lipofectin complex were NBT-positive and showed morphological changes with differentiated phenotype compared with 29% of untreated cells. The expression of the c-myc in the treated cells decreased markedly. The levels of c-myc protein after treatment decreased from (80.00 +/- 3.02)% to (41.67 +/- 4.56)% (P < 0.01). c-myc AS-ODN induced HL-60 cells apoptosis which morphological changes of apoptotic cells were investigated by fluorescence microsopy. A ladder-like pattern of DNA fragmentation was demonstrated on agorose gel electrophoresis and 17% apoptotic cells were found with flow cytometry.


CONCLUSIONS
Antisense oligodeoxynucleotide can suppress the malignant phenotype of HL-60 cells and enhancement of the effect was observed by adding lipofectin.
Chen
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L
Fujian Institute of Hematology, Union Hospital of Fujian Medical University, Fuzhou 350001, China.
Zhuo
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G
Chen
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Z
D017209:Apoptosis / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D016259:Genes, myc; D018922:HL-60 Cells / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D008081:Liposomes; D020319:Oligodeoxyribonucleotides, Antisense / Q000494:pharmacology*; D016271:Proto-Oncogene Proteins c-myc / Q000378:metabolism
D008081:Liposomes; D020319:Oligodeoxyribonucleotides, Antisense; D016271:Proto-Oncogene Proteins c-myc