Enhanced immunogenicity of a contraceptive vaccine using diverse synthetic carriers with permissible adjuvant.
A vaccine directed against human chorionic gonadotropin (hCG) has previously undergone clinical testing demonstrating the feasibility of the approach in preventing pregnancy in women. Some individuals, however, did not respond adequately despite employing highly immunogenic bacterial toxoids as carriers. We investigated the potential of three promiscuous pathogen-derived Th peptides as carriers, employing alum as the adjuvant. While conjugation with each peptide improved the antibody response against hCG in mice of different haplotypes, immunisation with a combination of these peptide-conjugates generated anti-hCG responses higher than those achieved with the individual peptides or tetanus toxoid (TT). Antibodies were of high affinity and capable of neutralising the bioactivity of hCG but were devoid of anti-peptide reactivity. These results have implication for the design of hCG vaccine with improved immunogenicity for diverse population.
Gupta
A
A
Immunoendocrinology Laboratory, National Institute of Immunology, 110067, New Delhi, India.
Pal
R
R
Ahlawat
S
S
Bhatia
P
P
Singh
O
O
D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000595:Amino Acid Sequence; D000818:Animals; D000917:Antibody Formation; D006063:Chorionic Gonadotropin / Q000276:immunology*; D005260:Female; D006183:H-2 Antigens / Q000235:genetics; D006239:Haplotypes; D006801:Humans; D007700:Kinetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D010455:Peptides / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D011247:Pregnancy; D013045:Species Specificity; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology; D013745:Tetanus Toxoid / Q000008:administration & dosage; D018074:Vaccines, Conjugate / Q000008:administration & dosage; D022642:Vaccines, Contraceptive / Q000008:administration & dosage*
D000276:Adjuvants, Immunologic; D006063:Chorionic Gonadotropin; D006183:H-2 Antigens; D010455:Peptides; D013745:Tetanus Toxoid; D018074:Vaccines, Conjugate; D022642:Vaccines, Contraceptive