Application of capillary electrophoresis with laser-induced fluorescence detection to the determination of biogenic amines and amino acids in brain microdialysate and homogenate samples.
A procedure is described to derivatize 16 primary-amine-containing biogenic amines and amino acids in brain mixtures with the fluorogenic reagent 5-furoylquinoline-3-carboxaldehyde (FQ). These FQ-tagged compounds in the brain sample were resolved in less than 16 min based on micellar electrokinetic chromatography and laser-induced fluorescence. There was a linear relationship between the concentration of analyte and the fluorescence intensity, with correlation coefficients in the range of 0.96-1.00. The utility of this method for the quantification of the important inhibitory neurotransmitter gamma-aminobutyric acid in microdialysates and brain homogenates from rats is illustrated.
Chen
Z
Z
Department of Chemistry, University of Alberta, Edmonton, Canada.
Wu
J
J
Baker
G B
GB
Parent
M
M
Dovichi
N J
NJ
D000596:Amino Acids / Q000032:analysis*; D000818:Animals; D000928:Antidepressive Agents / Q000008:administration & dosage; D001679:Biogenic Amines / Q000032:analysis*; D001923:Brain Chemistry; D002138:Calibration; D019075:Electrophoresis, Capillary / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry; D005663:Furans / Q000737:chemistry; D007834:Lasers; D008297:Male; D017551:Microdialysis; D010624:Phenelzine / Q000008:administration & dosage; D011804:Quinolines / Q000737:chemistry; D051381:Rats; D013050:Spectrometry, Fluorescence / Q000379:methods*
C413243:5-furoylquinoline-3-carboxaldehyde; D000596:Amino Acids; D000928:Antidepressive Agents; D001679:Biogenic Amines; D005456:Fluorescent Dyes; D005663:Furans; D011804:Quinolines; D010624:Phenelzine