Microsomal malonyl-CoA-sensitive carnitine acyltransferase.
Liver microsomes contain two carnitine acyltransferase activities. One of these has properties closely corresponding to those of 88 kDa mitochondrial carnitine palmitoyltransferase-1 (CPT-1). Antisera against CPT-1 cross-react with an 88 kDa microsomal protein, suggesting that CPT-1 may be targeted to both microsomal and mitochondrial membranes. However, no experiments using cDNAs corresponding to CPT-1 involving in vitro translation with microsomes or involving in vivo COS-1 cell transfection provided any evidence to support this hypothesis.
Broadway
N M
NM
Department of Biochemistry and Molecular Biology, University College London, Gower Street, London WC1E 6BT, U.K.
Pease
R J
RJ
Birdsey
G
G
Turner
N A
NA
Shayeghi
M
M
Saggerson
E D
ED
D000818:Animals; D002333:Carnitine Acyltransferases / Q000378:metabolism*; D002334:Carnitine O-Palmitoyltransferase / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000201:enzymology / Q000378:metabolism; D008099:Liver / Q000201:enzymology / Q000378:metabolism; D008316:Malonyl Coenzyme A / Q000378:metabolism*; D008862:Microsomes, Liver / Q000201:enzymology* / Q000378:metabolism; D008928:Mitochondria / Q000201:enzymology / Q000378:metabolism; D020033:Protein Isoforms / Q000378:metabolism; D021381:Protein Transport
D020033:Protein Isoforms; D008316:Malonyl Coenzyme A; D002333:Carnitine Acyltransferases; D002334:Carnitine O-Palmitoyltransferase