Pantothenic acid protects jurkat cells against ultraviolet light-induced apoptosis.
Human leukemic T lymphocytes (Jurkat cells) were induced to undergo apoptosis by brief irradiation with ultraviolet C light (254 nm). This was accompanied by accumulation of lipid peroxidation products in the form of conjugated dienes, a decrease of total glutathione content, and a shift of its redox state towards the oxidized form. Preincubation of the cells with 1 mM pantothenate resulted in a significant elevation of total glutathione content of the cells, reaching its maximum level, 160% of the control, after 3 h. Similar increase was observed after preincubation with 5 mM N-acetylcysteine, a known precursor of glutathione. Both pantothenic acid and N-acetylcysteine alleviated the ultraviolet-induced decrease of glutathione content, diminished lipid peroxidation, and partly protected the cells against apoptosis produced by ultraviolet irradiation.
Slyshenkov
V S
VS
Nencki Institute of Experimental Biology, Polish Academy of Sciences, Warsaw, Poland.
Piwocka
K
K
Sikora
E
E
Wojtczak
L
L
D000111:Acetylcysteine / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects* / Q000528:radiation effects*; D001562:Benzimidazoles; D019610:Cytoprotection / Q000187:drug effects*; D005609:Free Radicals / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D019169:Jurkat Cells / Q000187:drug effects* / Q000528:radiation effects; D015227:Lipid Peroxidation / Q000187:drug effects / Q000528:radiation effects; D010084:Oxidation-Reduction; D010205:Pantothenic Acid / Q000494:pharmacology*; D014466:Ultraviolet Rays
D001562:Benzimidazoles; D005609:Free Radicals; C447680:hoechst 32258; D010205:Pantothenic Acid; D005978:Glutathione; D000111:Acetylcysteine