title: Regulation of lactate production by FSH, iL1beta, and TNFalpha in rat Sertoli cells.
abstract: One of the "nurse cell" functions of Sertoli cells is to provide lactate for the energy production in spermatocytes and spermatids. The present study shows that, as in porcine Sertoli cells, interleukin (IL)1beta and follicle-stimulating hormone (FSH) increase lactate production in rat Sertoli cells (basal, 9.1 +/- 1.0; FSH (100 ng/ml), 16.6 +/- 2.0; IL1beta (50 ng/ml), 13.3 +/- 1.6 microg/microg DNA). Increments in glucose uptake (basal, 1083 +/- 70; FSH, 2686 +/- 128; IL1beta, 1899 +/- 74 dpm/microg DNA), lactic dehydrogenase (LDH) activity (basal, 36.6 +/- 4.1; FSH, 52.2 +/- 4.9; IL1beta, 55.3 +/- 5.1 mUI/microg DNA), LDH A mRNA levels, and redistribution of LDH isozymes are involved in these stimulatory effects. Differences in the period required by IL1beta to increase glucose uptake, as compared with the porcine model, have been observed. In addition, tumor necrosis factor alpha (TNFalpha), one of the major stimulators for lactate production in porcine Sertoli cells, does not control the secretion of this glucose metabolite in rat Sertoli cells. Lactate production may be regulated differently among mammals.
authors.affiliation: Centro de Investigaciones Endocrinológicas (CEDIE), Hospital de Niños "R Gutiérrez,", Gallo 1330, Buenos Aires, 1425, Argentina.
authors.forename: M F
authors.initials: MF
authors.lastname: Riera
authors.forename: S B
authors.initials: SB
authors.lastname: Meroni
authors.forename: G E
authors.initials: GE
authors.lastname: Gómez
authors.forename: H F
authors.initials: HF
authors.lastname: Schteingart
authors.forename: E H
authors.initials: EH
authors.lastname: Pellizzari
authors.forename: S B
authors.initials: SB
authors.lastname: Cigorraga
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D005640:Follicle Stimulating Hormone / Q000494:pharmacology*; D005947:Glucose / Q000378:metabolism; D007375:Interleukin-1 / Q000494:pharmacology*; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000235:genetics / Q000378:metabolism; D019344:Lactic Acid / Q000096:biosynthesis*; D008297:Male; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D012708:Sertoli Cells / Q000187:drug effects / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
chemical_list: D007375:Interleukin-1; D007527:Isoenzymes; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D019344:Lactic Acid; D005640:Follicle Stimulating Hormone; D007770:L-Lactate Dehydrogenase; D005947:Glucose