The Saccharomyces cerevisiae RAD9 cell cycle checkpoint gene is required for optimal repair of UV-induced pyrimidine dimers in both G(1) and G(2)/M phases of the cell cycle.
Cells respond to DNA damage by activating both cellular growth arrest and DNA repair processes. In Saccharomyces cerevesiae the RAD9 gene controls DNA damage-mediated cell cycle arrest that is known to allow efficient repair. To ascertain whether RAD9 plays a role in DNA repair per se, the removal of UV-induced photolesions was assessed in synchronized isogenic normal and rad9 cells using the high resolution primer extension technique. The results show that RAD9 is indeed involved in the removal of photolesions from both the transcribed and the non-transcribed strands of the reporter GAL10 gene, in G(1)- as well as G(2)/M-arrested cells. Interestingly, these data also reveal that in both normal and rad9 mutant, the repair strand bias towards the transcribed stand is more pronounced in G(2)/M- than in G(1)-arrested cells. These data indicate that RAD9 coordinate the cellular response to DNA damage by activating both cell cycle checkpoint and excision repair.
Al-Moghrabi
N M
NM
King Faisal Specialist Hospital and Research Center, Department of Biological and Medical Research, MBC-03, PO Box 3354, Riyadh 11211, Kingdom of Saudi Arabia.
Al-Sharif
I S
IS
Aboussekhra
A
A
D002453:Cell Cycle / Q000187:drug effects / Q000528:radiation effects; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D004249:DNA Damage / Q000235:genetics / Q000528:radiation effects*; D004260:DNA Repair / Q000187:drug effects / Q000235:genetics*; D016193:G1 Phase / Q000187:drug effects / Q000528:radiation effects; D016195:G2 Phase / Q000187:drug effects / Q000528:radiation effects; D017353:Gene Deletion; D005800:Genes, Fungal / Q000235:genetics; D017930:Genes, Reporter / Q000235:genetics; D000072235:Mating Factor; D008938:Mitosis / Q000187:drug effects / Q000528:radiation effects; D010455:Peptides / Q000494:pharmacology; D011740:Pyrimidine Dimers / Q000235:genetics* / Q000528:radiation effects; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects; D014158:Transcription, Genetic / Q000187:drug effects / Q000235:genetics; D014466:Ultraviolet Rays
D018797:Cell Cycle Proteins; D010455:Peptides; D011740:Pyrimidine Dimers; C069930:rad9 protein; D000072235:Mating Factor