Radiobiological effects of a low-energy ion beam on wheat.
The radiobiological effects of a low-energy nitrogen ion (N+) beam on wheat were studied, particularly with regard to the induction of chromosome aberrations. The results demonstrated that the three test varieties showed different sensitivities to ion implantation, and a higher dose of ion implantation had a marked effect on the germination and survival rate of the seeds exposed. The germination rate and survival rate curve basically followed a similar trend in the same variety. Cytological analysis indicated that ion beams were effective in producing chromosome aberrations. The frequencies of mitotic or meiotic cells with chromosome aberrations increased linearly with increasing doses. The aberration types included, for example, acentric fragments, chromosome deletions, lagging chromosomes, chromosome bridges and micronuclei. In the root tip cells, aberrations chiefly consisted of acentric fragments and deletions. Chromosome bridges and lagging chromosomes were the main aberration phenomena observed in the pollen mother cells. The highest frequencies of root tip cells and pollen mother cells with chromosome aberrations were 15.2% and 39.8%, respectively. Changes in morphology and mutant were also observed in the plants derived from exposed seeds.
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Laboratory of Ion Beam Bioengineering, Institute of Plasma Physics, Chinese Academy of Sciences, P.O. Box 1126, Hefei 230031, Anhui, PR China. cdhu@mail.ipp.ac.cn
Yu
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D002869:Chromosome Aberrations; D002872:Chromosome Deletion; D002875:Chromosomes / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D007621:Karyotyping; D048629:Micronuclei, Chromosome-Defective / Q000528:radiation effects; D008940:Mitotic Index; D009584:Nitrogen; D018517:Plant Roots / Q000528:radiation effects; D012639:Seeds / Q000502:physiology / Q000528:radiation effects; D014908:Triticum / Q000235:genetics / Q000502:physiology / Q000528:radiation effects*
D009584:Nitrogen