Bone marrow-derived macrophages grown in GM-CSF or M-CSF differ in their ability to produce IL-12 and to induce IFN-gamma production after stimulation with Trypanosoma cruzi antigens.
Trypanosoma cruzi is the etiological agent of Chagas' disease in man. Control of parasitism at the beginning of experimental infection depends on cytokine-activated macrophages that synthesize nitric oxide (NO). We investigated macrophage populations derived in the presence of M-CSF (M-MØ) or GM-CSF (GM-MØ) regarding their ability to control intracellular parasitism by T. cruzi and to synthesize IL-12 and NO. Both macrophage populations supported intracellular multiplication of the parasite; when activated by IFN-gamma, GM-MØ exerted better control of parasitism. Stimulation of GM-MØ with T. cruzi or Staphylococcus aureus resulted in IL-12 production and higher levels of NO synthesis in comparison with stimulated M-MØ. Mice immunized with parasite-Ag-pulsed GM-MØ but not with pulsed M-MØ had increased IFN-gamma and IL-2 production in lymph nodes. However, when immunization was followed by infection with live parasites, transient elevation of IFN-gamma production was observed in both GM-MØ- and M-MØ-immunized mice, without reduction of blood parasite levels.
Tadokoro
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Departamento de Imunologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, Av. Prof. Lineu Prestes, 1730, Ed. Biomédicas IV, São Paulo, 05508-900, SP, Brazil.
de Almeida Abrahamsohn
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D000818:Animals; D000953:Antigens, Protozoan / Q000276:immunology*; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects / Q000276:immunology* / Q000469:parasitology; D002455:Cell Division; D002478:Cells, Cultured; D014355:Chagas Disease / Q000517:prevention & control; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000276:immunology* / Q000494:pharmacology; D007371:Interferon-gamma / Q000096:biosynthesis*; D018664:Interleukin-12 / Q000096:biosynthesis*; D008198:Lymph Nodes; D016173:Macrophage Colony-Stimulating Factor / Q000276:immunology* / Q000494:pharmacology; D008264:Macrophages / Q000166:cytology / Q000187:drug effects / Q000276:immunology* / Q000469:parasitology; D051379:Mice; D008807:Mice, Inbred BALB C; D009569:Nitric Oxide / Q000096:biosynthesis; D014349:Trypanosoma cruzi / Q000254:growth & development / Q000276:immunology*; D014611:Vaccination
D000953:Antigens, Protozoan; D018664:Interleukin-12; D009569:Nitric Oxide; D016173:Macrophage Colony-Stimulating Factor; D007371:Interferon-gamma; D016178:Granulocyte-Macrophage Colony-Stimulating Factor