Comparison of MB/BacT system and agar proportion method in drug susceptibility testing of Mycobacterium tuberculosis.
The drug susceptibilities of 105 isolates/strains of Mycobacterium tuberculosis (101 clinical isolates and four control strains from the American Type Culture Collection) were assessed by the MB/BacT system and conventional agar proportion method. The agreement rates between the two methods were 99.0% for streptomycin, 95.2% for isoniazid and 100% for rifampin. The mean times to detection for drug-resistant isolates were 4.7 days (range: 2.5-13.7 days) using the MB/BacT system and 14.8 days (range: 14-21 days) using the agar proportion method. For drug-susceptible isolates, the times to detection were 10.8 days (mean) and 21 days respectively. Thus, these data have demonstrated that the automated, non-radiometric MB/BacT system is an efficient, accurate and reliable method for assessing drug susceptibilities of M. tuberculosis compared with the conventional agar proportion method.
Yew
W W
WW
Tuberculosis & Chest Unit, Grantham Hospital, 125 Wong Chuk Hang Road, Aberdeen, Hong Kong, China
Tong
S C
SC
Lui
K S
KS
Leung
S K
SK
Chau
C H
CH
Wang
E P
EP
D000904:Antibiotics, Antitubercular / Q000494:pharmacology*; D015374:Biosensing Techniques; D002245:Carbon Dioxide / Q000032:analysis; D015169:Colony Count, Microbial; D003124:Colorimetry / Q000379:methods; D004352:Drug Resistance, Microbial; D006801:Humans; D007538:Isoniazid / Q000494:pharmacology; D008826:Microbial Sensitivity Tests / Q000379:methods*; D009169:Mycobacterium tuberculosis / Q000187:drug effects*; D012293:Rifampin / Q000494:pharmacology; D013307:Streptomycin / Q000494:pharmacology
D000904:Antibiotics, Antitubercular; D002245:Carbon Dioxide; D007538:Isoniazid; D012293:Rifampin; D013307:Streptomycin