Serum prolactin is associated with apoptosis in men with human immunodeficiency virus infection.
We examined the in vivo and in vitro production of prolactin (PRL) in 20 untreated HIV-infected men compared to 14 uninfected men and its association with the cell cycle and apoptosis. Compared to uninfected men, the HIV-infected men had: (i) higher fasting serum bioactive (BIO) PRL; (ii) lower serum immunoreactive (RIA) and BIO-PRL responses to intravenous metoclopramide; (iii) greater BIO-RIA PRL ratio both fasting and during intravenous metoclopramide; (iv) lower percentage of non-stimulated PBMC in the G0/G1 phase, but a higher percentage in the S phase, of the cell cycle with normal response to Concanavalin-A; and (v) higher in vitro production of BIO-PRL by non-stimulated PBMC, which was blocked after Concanavalin-A. Fasting serum BIO-PRL positively correlated with the percent of non-stimulated PBMC in S + G2/M phases. The percentage of apoptotic PBMC negatively correlated with CD4+ T lymphocytes and with the area under the serum RIA-PRL curve, but positively correlated with the area under the curve for the BIO/RIA ratio. These results suggest that in these HIV-infected men: (i) a diminished dopaminergic tone may exist, as an adaptive mechanism attempting to survive; and (ii) BIO-PRL may participate as a cofactor in the stimulation of T-cell proliferation.
Parra
A
A
Department of Endocrinology, Instituto Nacional de Perinatología, Mexico City, México. parra12@hotmail.com
Ramírez-Peredo
J
J
Larrea
F
F
Pérez-Romano
B
B
Cabrera
V
V
Torres
I
I
Reyes-Núñez
V
V
Ruiz-Argüelles
G
G
Ruiz-Argüelles
A
A
D000328:Adult; D000818:Animals; D017209:Apoptosis; D019540:Area Under Curve; D018791:CD4 Lymphocyte Count; D015496:CD4-Positive T-Lymphocytes / Q000502:physiology; D002453:Cell Cycle; D002478:Cells, Cultured; D018492:Dopamine Antagonists / Q000494:pharmacology; D006678:HIV / Q000378:metabolism*; D015658:HIV Infections / Q000097:blood / Q000503:physiopathology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism / Q000502:physiology*; D008297:Male; D008787:Metoclopramide / Q000494:pharmacology; D011388:Prolactin / Q000097:blood*; D011863:Radioimmunoassay; D013223:Statistics as Topic
D018492:Dopamine Antagonists; D011388:Prolactin; D008787:Metoclopramide