Determination of trace amounts of lead in mussels by flow-injection flame atomic-absorption spectrometry coupled with on-line minicolumn preconcentration.
A minicolumn packed with poly(aminophosphonic acid) chelating resin incorporated in an on-line preconcentration system for flame atomic-absorption spectrometry was used to determine ultratrace amounts of lead in mussel samples at microg L(-1) level. The preconcentrated lead was eluted with hydrochloric acid and injected directly into the nebulizer for atomization in an air-acetylene flame for measurement. The performance characteristics of the determination of lead were: preconcentration factor 26.8 for 1 min preconcentration time, detection limit (3sigma) in the sample digest was 0.25 microg g(-1) (dry weight) for a sample volume of 3.5 mL and 0.2 g sample (preconcentration time 1 min), precision (RSD) 2.3% for 25 microg L(-1) and 2.0% for 50 microg L(-1). The sampling frequency was 45 h(-1). The method was highly tolerant of interferences, and the results obtained for the determination of lead in a reference material testify to the applicability of the proposed procedure to the determination of lead at ultratrace level in biological materials such as mussel samples.
Yebra
M C
MC
Department of Analytical Chemistry, Nutrition and Bromatology, Chemistry Faculty, University of Santiago, Santiago de Compostela, Spain. qncayebi@usc.es
Enríquez
M F
MF
García
A
A
Moreno-Cid
A
A
D000818:Animals; D049872:Bivalvia / Q000737:chemistry*; D002614:Chelating Agents / Q000737:chemistry; D006851:Hydrochloric Acid / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007854:Lead / Q000032:analysis*; D063065:Organophosphonates / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D012015:Reference Standards; D013030:Spain; D013054:Spectrophotometry, Atomic / Q000295:instrumentation* / Q000379:methods*; D014131:Trace Elements / Q000032:analysis*
D002614:Chelating Agents; D063065:Organophosphonates; D011108:Polymers; D014131:Trace Elements; C430363:poly(aminophosphonic acid); D007854:Lead; D006851:Hydrochloric Acid