[Detection of antibodies to the nucleocapsid protein of Hepatitis C virus by immunoenzyme analysis using synthetic peptides of nucleocapsid N-terminal part].
Solid-phase synthesis of 7 linear peptides overlapping the immunodominant N-terminal region of hepatitis C virus (HCV) core protein (aa 7-75) was carried out. Their antigen reactivity was studied by non-competitive ELISA with sera from patients with chronic HCV infection. Three B-epitopes located within aa 7-19, 20-34, and 39-75 appeared to be the most immunoreactive. Use of the set of synthetic peptides in ELISA detected anti-HCV core antibodies with 93% efficiency in comparison with ELISA and commercial anti-HCV Recomblot based on the use of commercial recombinant HCV core protein.
Firsova
T V
TV
Alekseenkova
T I
TI
Shibnev
V A
VA
Froesner
G
G
Semiletov
Iu A
IuA
D004797:Enzyme-Linked Immunosorbent Assay; D016174:Hepacivirus / Q000276:immunology*; D006526:Hepatitis C / Q000175:diagnosis* / Q000276:immunology / Q000821:virology; D018936:Hepatitis C Antigens / Q000276:immunology; D006801:Humans; D016056:Immunodominant Epitopes / Q000737:chemistry / Q000276:immunology; D010455:Peptides / Q000737:chemistry / Q000276:immunology; D014758:Viral Core Proteins / Q000737:chemistry / Q000276:immunology*
D018936:Hepatitis C Antigens; D016056:Immunodominant Epitopes; D010455:Peptides; D014758:Viral Core Proteins; C069130:nucleocapsid protein, Hepatitis C virus