title: Characterization of Escherichia coli type 1 pilus mutants with altered binding specificities.
abstract: PCR mutagenesis and a unique enrichment scheme were used to obtain two mutants, each with a single lesion in fimH, the chromosomal gene that encodes the adhesin protein (FimH) of Escherichia coli type 1 pili. These mutants were noteworthy in part because both were altered in the normal range of cell types bound by FimH. One mutation altered an amino acid at a site previously shown to be involved in temperature-dependent binding, and the other altered an amino acid lining the predicted FimH binding pocket.
authors.affiliation: Department of Microbiology, Pathology, and Parasitology, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina 27606, USA.
authors.forename: S L
authors.initials: SL
authors.lastname: Harris
authors.forename: P A
authors.initials: PA
authors.lastname: Spears
authors.forename: E A
authors.initials: EA
authors.lastname: Havell
authors.forename: T S
authors.initials: TS
authors.lastname: Hamrick
authors.forename: J R
authors.initials: JR
authors.lastname: Horton
authors.forename: P E
authors.initials: PE
authors.lastname: Orndorff
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics / Q000378:metabolism*; D018830:Adhesins, Escherichia coli; D000483:Alleles; D002876:Chromosomes, Bacterial; D004926:Escherichia coli / Q000502:physiology*; D036781:Fimbriae Proteins; D010861:Fimbriae, Bacterial / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009154:Mutation; D010641:Phenotype; D010957:Plasmids; D011995:Recombination, Genetic
chemical_list: D018829:Adhesins, Bacterial; D018830:Adhesins, Escherichia coli; C058769:fimH protein, E coli; D036781:Fimbriae Proteins