Effects of Gabapentin and Topiramate in primary rat astrocyte cultures.
We studied in vitro the effects of anticonvulsant drugs Gabapentin and Topiramate on the production of reactive oxygen species and nitric oxide (NO), the activity of glutamine synthetase (GS), and cell viability in primary cultures of rat cortical astrocytes which are intimately involved in the normal functioning of neurons. We investigated the effects of these drugs at concentrations within the therapeutic range (1, 10 and 50 microg/ml). We observed that, in cultured astrocytes, Gabapentin induced a weak increase in the biosynthesis of NO, a mild decrease in GS activity and cell viability, and minor induction of a stress condition. Topiramate was observed to induce even greater stressor effects on these cells.
Cardile
V
V
Department of Physiological Sciences, University of Catania, Italy.
Pavone
A
A
Renis
M
M
Maci
T
T
Perciavalle
V
V
D000085:Acetates / Q000494:pharmacology*; D000588:Amines; D000818:Animals; D000927:Anticonvulsants / Q000494:pharmacology*; D001253:Astrocytes / Q000187:drug effects* / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003509:Cyclohexanecarboxylic Acids; D005632:Fructose / Q000031:analogs & derivatives* / Q000494:pharmacology*; D000077206:Gabapentin; D005974:Glutamate-Ammonia Ligase / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D009569:Nitric Oxide / Q000378:metabolism; D009994:Osmolar Concentration; D018384:Oxidative Stress; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D000077236:Topiramate; D005680:gamma-Aminobutyric Acid
D000085:Acetates; D000588:Amines; D000927:Anticonvulsants; D003509:Cyclohexanecarboxylic Acids; D008070:Lipopolysaccharides; D017382:Reactive Oxygen Species; D000077236:Topiramate; D005632:Fructose; D009569:Nitric Oxide; D005680:gamma-Aminobutyric Acid; D000077206:Gabapentin; D007770:L-Lactate Dehydrogenase; D005974:Glutamate-Ammonia Ligase