Evaluation of retinal damage induced by air/fluid exchange using a trypan blue inclusion test in rabbits.
OBJECTIVE
To investigate whether retina damaged in the process of air/fluid exchange could be visualized in situ using trypan blue staining.


METHODS
Vitrectomy and air/fluid exchange with continuous air infusion for various periods of time was performed in adult pigmented rabbits, followed by injection of 0.4% trypan blue solution into the vitreous cavity. Retinal staining was evaluated intraoperatively under the operating microscope and by light microscopy after enucleation.


RESULTS
Intraoperative examination showed localized trypan blue staining of areas of the retina opposite the infusion cannula. Light microscopy of the same areas revealed intranuclear trypan blue inclusion in the retina.


CONCLUSIONS
Trypan blue staining allows for the immediate in situ evaluation of damage to the retina associated with air/fluid exchange and continuous air infusion in rabbit eyes.
Oshitari
K
K
Kyorin Eye Center, Mitaka, Tokyo, Japan. oshitara@eye-center.org
Hirakata
A
A
Nagamoto
T
T
Okada
A A
AA
Hida
T
T
D000388:Air; D000818:Animals; D004396:Coloring Agents; D007267:Injections; D011184:Postoperative Period; D011817:Rabbits; D012160:Retina / Q000473:pathology*; D012965:Sodium Chloride; D014343:Trypan Blue; D014821:Vitrectomy
D004396:Coloring Agents; D012965:Sodium Chloride; D014343:Trypan Blue