Imaging of various surface properties of fluorescently labelled phospholipid Langmuir-Blodgett films with a combined scanning probe microscope.
We present results of phase separation of a single-component system of 1,2-dihexadecanoyl-sn-glycero-3-phospho-[N-(4-nitrobenz)-2-oxa-1,3-diazolyl]ethanolamine in which a liquid-condensed (LC) phase co-exists with a liquid-expanded (LE) phase. Domain formation in the co-existence region was studied using a newly developed combined scanning near-field optical microscope-atomic force microscope (SNOM-AFM). We demonstrate for the first time that the topographic, friction, fluorescence and surface potential distributions for a phase-separated single-component Langmuir-Blodgett film between the LE and LC phases can be simultaneously observed using the SNOM-AFM with a thin-step etched optical fibre probe.
Horiuchi
Y
Y
Department of Biomolecular Engineering, Tokyo Institute of Technology, Yokohama, Japan.
Yagi
K
K
Hosokawa
T
T
Yamamoto
N
N
Muramatsu
H
H
Fujihira
M
M
D005456:Fluorescent Dyes; D008853:Microscopy / Q000379:methods; D010714:Phosphatidylethanolamines / Q000737:chemistry*
C430567:1,2-dihexadecanoyl-sn-glycero-3-phospho-(N-(4-nitrobenz)-2-oxa-1,3-diazolyl)ethanolamine; D005456:Fluorescent Dyes; D010714:Phosphatidylethanolamines