Serial analysis of gene expression in chronic hepatitis C and hepatocellular carcinoma.
Hepatitis C virus (HCV) causes chronic hepatitis C (CH-C) and is epidemiologically linked with the occurrence of hepatocellular carcinoma (HCC). To elucidate the comprehensive gene expression profiles of CH-C and HCC, serial analysis of gene expression (SAGE) libraries were made from CH-C and HCC tissues of a patient, and compared with a reported SAGE library of a normal liver (NL). Scatter plots of the distribution of tags from the HCC library exhibited the existence of many differentially expressed genes compared with those from the CH-C and NL libraries. Up-regulation of IFN-gamma inducible genes and oxidative stress-inducible genes were identified in both the CH-C and HCC libraries, and some unpublished new genes were specifically up- or down-regulated in the HCC library. This genome-wide scanning study discloses the molecular portraits of CH-C and HCC, and provides novel candidate genes that should help clarify the mechanism of hepatocarcinogenesis in the chronically HCV-infected liver.
Yamashita
T
T
First Department of Internal Medicine & CREST, Kanazawa University School of Medicine, Kanazawa, 920-8641, Japan
Kaneko
S
S
Hashimoto
S
S
Sato
T
T
Nagai
S
S
Toyoda
N
N
Suzuki
T
T
Kobayashi
K
K
Matsushima
K
K
D001483:Base Sequence; D006528:Carcinoma, Hepatocellular / Q000209:etiology* / Q000235:genetics*; D016022:Case-Control Studies; D017931:DNA Primers / Q000235:genetics; D020224:Expressed Sequence Tags; D015870:Gene Expression; D020869:Gene Expression Profiling; D015723:Gene Library; D016147:Genes, Tumor Suppressor; D019698:Hepatitis C, Chronic / Q000150:complications* / Q000235:genetics*; D006801:Humans; D007372:Interferons / Q000235:genetics; D008113:Liver Neoplasms / Q000209:etiology* / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009857:Oncogenes; D018384:Oxidative Stress / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
D017931:DNA Primers; D007372:Interferons