Quantitative comparison of two types of planar lipid bilayers--folded and painted--with respect to fusion with vesicles.
Two major types of planar lipid bilayers, painted and folded, were compared with respect to vesicle fusion using one chamber for the preparation of both bilayers. Liposomes containing ion channels composed of nystatin and ergosterol were used as the vesicle sample. Fusion of the liposome to either bilayer elicited a spike-like current change, which corresponds to a fusion event. The lag time between the first fusion event and the addition of the vesicles is an index of the ease with which the vesicles fuse with the bilayers. The lag time in the painted bilayer at a KCl concentration (cis) of 450 mM was 1.58+/-1.18 min, similar to that in the folded bilayer (1.65+/-0.64 min). The lag time decreased with increase of the osmotic difference across the painted bilayer, whereas this change was small in the folded bilayer. The fusion of the liposomes to the painted bilayer was markedly reduced by stopping the stirring in the cis compartment, whereas the fusion to the folded bilayer was not affected significantly. These results imply that no practical difference exists in the ability of vesicles to fuse with the painted and folded bilayers. For the study of single channel behavior, the painted bilayer could have an advantage because simply stopping the stirring prevents excess fusion.
Anzai
K
K
Bioregulation Research Group, National Institute of Radiological Sciences, 4-9-1 Anagawa, Chiba 263-8555, Inage, Japan. anzai@nirs.go.jp
Ogawa
K
K
Ozawa
T
T
Yamamoto
H
H
D000818:Animals; D001703:Biophysics / Q000379:methods*; D002417:Cattle; D004531:Eggs; D008051:Lipid Bilayers / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry; D009995:Osmosis; D010713:Phosphatidylcholines / Q000737:chemistry; D011189:Potassium Chloride / Q000494:pharmacology; D013997:Time Factors
D008051:Lipid Bilayers; D008081:Liposomes; D010713:Phosphatidylcholines; D011189:Potassium Chloride