title: Mixed epithelial and stromal tumor of the kidney lacks the genetic alterations of cellular congenital mesoblastic nephroma.
abstract: Mixed epithelial and stromal tumor of the kidney is a recently recognized neoplasm that occurs almost exclusively in perimenopausal women. Because it frequently contains areas of smooth muscle in which epithelial structures are embedded, some have concluded that it is the adult form of congenital mesoblastic nephroma. Others have concluded that the morphology and epidemiology of mixed epithelial and stromal tumor indicate that it is unrelated to congenital mesoblastic nephroma. Although the genetic alterations of mixed epithelial and stromal tumor have not been previously elucidated, much is known about the genetic alterations of cellular congenital mesoblastic nephroma. The present study was undertaken to determine if mixed epithelial and stromal tumors have any of the genetic alterations recognized as typical of cellular congenital mesoblastic nephroma. RNA extraction was performed on formalin-fixed, paraffin-embedded tissue from 7 mixed epithelial and stromal tumors followed by reverse-transcription polymerase chain reaction to detect the ETV6-NTRK3 gene fusion. Fluorescent in situ hybridization with centromere-specific probes for chromosomes 8, 11, and 17 was performed to evaluate polyploidy of these chromosomes in 11 cases of mixed epithelial and stromal tumor. None of the mixed epithelial and stromal tumors showed any of these genetic alterations. We conclude that mixed epithelial and stromal tumor of the kidney lacks the genetic alterations typical of cellular congenital mesoblastic nephroma, is unrelated to it, and the appellation "adult mesoblastic nephroma" should not be used for these tumors.
authors.affiliation: Harper Hospital and Karmanos Cancer Institute, Wayne State University, Detroit, MI 48201, USA.
authors.forename: C R
authors.initials: CR
authors.lastname: Pierson
authors.forename: M S
authors.initials: MS
authors.lastname: Schober
authors.forename: T
authors.initials: T
authors.lastname: Wallis
authors.forename: F H
authors.initials: FH
authors.lastname: Sarkar
authors.forename: P H
authors.initials: PH
authors.lastname: Sorensen
authors.forename: J N
authors.initials: JN
authors.lastname: Eble
authors.forename: J R
authors.initials: JR
authors.lastname: Srigley
authors.forename: E C
authors.initials: EC
authors.lastname: Jones
authors.forename: D J
authors.initials: DJ
authors.lastname: Grignon
authors.forename: V
authors.initials: V
authors.lastname: Adsay
mesh_terms: D000328:Adult; D000368:Aged; D002880:Chromosomes, Human, Pair 11; D002886:Chromosomes, Human, Pair 17; D002898:Chromosomes, Human, Pair 8; D004268:DNA-Binding Proteins / Q000235:genetics; D004847:Epithelial Cells / Q000473:pathology*; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007680:Kidney Neoplasms / Q000235:genetics* / Q000473:pathology; D008593:Menopause; D008875:Middle Aged; D018201:Nephroma, Mesoblastic / Q000151:congenital* / Q000235:genetics*; D015514:Oncogene Proteins, Fusion; D011003:Ploidies; D050783:Proto-Oncogene Proteins c-ets; D020812:Receptor, trkC / Q000235:genetics; D012097:Repressor Proteins; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017154:Stromal Cells / Q000473:pathology*; D014157:Transcription Factors / Q000235:genetics; D014178:Translocation, Genetic; D000097764:ETS Translocation Variant 6 Protein
chemical_list: D004268:DNA-Binding Proteins; D015514:Oncogene Proteins, Fusion; D050783:Proto-Oncogene Proteins c-ets; D020812:Receptor, trkC; D012097:Repressor Proteins; D014157:Transcription Factors; D000097764:ETS Translocation Variant 6 Protein