title: Imaging infection with LeuTech.
abstract: LeuTech is a 99Tcm labelled, anti-CD15, IgM, murine monoclonal antibody shown to have high affinity (Kd = 10(-11) M) for CD15 receptors (5.1 x 10(5)/cell) expressed on human neutrophils. LeuTech was injected directly, intravenously, and its efficacy in imaging infection in 46 consecutive patients was determined. Human anti-mouse antibody (HAMA) response was examined in 30 normal volunteers using a standard LeuTech dose reconstituted with decayed 99Tcm solution. There were 38 true positive, six true negative, and two false negative scans. Of the 38 positive images, 33 (92%) were positive within 10 min after injection of LeuTech. LeuTech accuracy in this group of patients was 96%, sensitivity 95%, specificity 100%, positive predictive value (PPV) 100%, and negative predictive value (NPV) 75%. No elevation of the HAMA titre was observed in any of the 30 normal volunteers and no adverse reaction was noted in any patient. LeuTech is a highly promising agent for rapid imaging of infectious foci.
authors.affiliation: Department of Radiology, Thomas Jefferson University, Philadelphia, PA 19107, USA. Mathew.Thakur@mail.tju.edu
authors.forename: M L
authors.initials: ML
authors.lastname: Thakur
authors.forename: C S
authors.initials: CS
authors.lastname: Marcus
authors.forename: S L
authors.initials: SL
authors.lastname: Kipper
authors.forename: R
authors.initials: R
authors.lastname: Ahdoot
authors.forename: L
authors.initials: L
authors.lastname: Diggles
authors.forename: H L
authors.initials: HL
authors.lastname: Pham
authors.forename: C
authors.initials: C
authors.lastname: Minami
authors.lastname: Rhodes
authors.forename: M C
authors.initials: MC
authors.lastname: Patel
authors.forename: T
authors.initials: T
authors.lastname: Smith
mesh_terms: D000328:Adult; D000818:Animals; D000911:Antibodies, Monoclonal; D001064:Appendicitis / Q000000981:diagnostic imaging; D001847:Bone Diseases / Q000000981:diagnostic imaging; D005188:False Negative Reactions; D005260:Female; D006801:Humans; D007239:Infections / Q000000981:diagnostic imaging*; D016256:Lewis X Antigen / Q000276:immunology; D008171:Lung Diseases / Q000000981:diagnostic imaging; D008297:Male; D051379:Mice; D008875:Middle Aged; D011237:Predictive Value of Tests; D019275:Radiopharmaceuticals; D012016:Reference Values; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D015899:Tomography, Emission-Computed, Single-Photon
chemical_list: D000911:Antibodies, Monoclonal; D016256:Lewis X Antigen; D019275:Radiopharmaceuticals; C414128:techneticum TC-99M fanolesomab