title: De novo-designed peptide transforms Golgi-specific lipids into Golgi-like nanotubules.
abstract: Cellular organelles, such as the Golgi apparatus and the endoplasmic reticulum, adopt characteristic structures depending on their function. While the tubular shapes of these structures result from complex protein-lipid interactions that are not fully understood, some fundamental machinery must be required. We show here that a de novo-designed 18-mer amphipathic alpha-helical peptide, Hel 13-5, transforms spherical liposomes made from a Golgi-specific phospholipid mixture into nanotubules on the scale of and resembling the shape of the nanotubules that form the Golgi apparatus. Furthermore, we show that that the size and the shape of such nanotubules depend on lipid composition and peptide properties such as length and the ratio of hydrophobic to hydrophilic amino acids. Although the question of precisely how nature engineers organellar membranes remains unknown, our simple novel system provides a basic set of tools to begin addressing this question.
authors.affiliation: Department of Chemistry, Faculty of Science, Fukuoka University, Fukuoka 814-0180, Japan. leesan@cis.fukuoka-u.ac.jp
authors.forename: S
authors.initials: S
authors.lastname: Lee
authors.forename: T
authors.initials: T
authors.lastname: Furuya
authors.forename: T
authors.initials: T
authors.lastname: Kiyota
authors.forename: N
authors.initials: N
authors.lastname: Takami
authors.forename: K
authors.initials: K
authors.lastname: Murata
authors.forename: Y
authors.initials: Y
authors.lastname: Niidome
authors.forename: D E
authors.initials: DE
authors.lastname: Bredesen
authors.forename: H M
authors.initials: HM
authors.lastname: Ellerby
authors.forename: G
authors.initials: G
authors.lastname: Sugihara
mesh_terms: D002942:Circular Dichroism; D006056:Golgi Apparatus / Q000378:metabolism*; D050356:Lipid Metabolism; D008854:Microscopy, Electron; D010455:Peptides / Q000737:chemistry / Q000378:metabolism*
chemical_list: C440034:Hel 13-5 peptide; D010455:Peptides