title: Effects of riluzole on N-methyl-D-aspartate-induced tyrosine phosphorylation in the rat hippocampus.
abstract: Since increased tyrosine phosphorylation has been observed in response to brain ischemia, we investigated whether riluzole (an inhibitor of glutamate neurotransmission with neuroprotective properties) affects tyrosine phosphorylation stimulated by N-methyl-D-aspartate (NMDA) in rat hippocampal slices. Riluzole produced an extremely potent concentration-related inhibition of NMDA (1 mM)-stimulated protein tyrosine phosphorylation (IC(50)=0.5+/-0.03 microM, mean+/-S.D.), but failed to affect that evoked by phorbol 12-myristate 13-acetate (PMA, an activator of protein kinase C, 0.1 and 1 microM). These results suggest that inhibition of tyrosine phosphorylation may contribute to the neuroprotective effects of riluzole against excitotoxic injury.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale U 408, Faculté Xavier Bichat, 16 rue Henri Huchard, 75018 Paris, France.
authors.forename: A
authors.initials: A
authors.lastname: Peyclit
authors.forename: H
authors.initials: H
authors.lastname: Keita
authors.forename: P
authors.initials: P
authors.lastname: Juvin
authors.forename: P
authors.initials: P
authors.lastname: Derkinderen
authors.forename: F
authors.initials: F
authors.lastname: Jardinaud
authors.forename: D
authors.initials: D
authors.lastname: Rouellé
authors.forename: J
authors.initials: J
authors.lastname: Boczkowski
authors.forename: J M
authors.initials: JM
authors.lastname: Desmonts
authors.forename: J A
authors.initials: JA
authors.lastname: Girault
authors.forename: J
authors.initials: J
authors.lastname: Mantz
mesh_terms: D000818:Animals; D002273:Carcinogens / Q000494:pharmacology; D018690:Excitatory Amino Acid Agonists / Q000494:pharmacology*; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D016202:N-Methylaspartate / Q000494:pharmacology*; D018696:Neuroprotective Agents / Q000494:pharmacology*; D009498:Neurotoxins / Q000378:metabolism; D010766:Phosphorylation; D011233:Precipitin Tests; D011493:Protein Kinase C / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D017470:Receptors, Glutamate / Q000378:metabolism; D019782:Riluzole / Q000494:pharmacology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014443:Tyrosine / Q000378:metabolism*
chemical_list: D002273:Carcinogens; D018690:Excitatory Amino Acid Agonists; D018696:Neuroprotective Agents; D009498:Neurotoxins; D017470:Receptors, Glutamate; D014443:Tyrosine; D016202:N-Methylaspartate; D019782:Riluzole; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate