From the chromosome to DNA: Restriction fragment length polymorphism analysis and its clinical application.
Understanding how chromosomal alterations contribute to acquired and inherited human disease requires the ability to manage the enormous physical and informational complexity of the deoxyribonucleic acid (DNA) packaged within. Important concepts and techniques involved in the analysis of DNA include restriction enzymes, Southern blotting, and restriction fragment length polymorphism/linkage analysis. These techniques have been essential in the understanding and diagnosis of several syndromes associated with the head and neck. The purpose of this article is to introduce DNA structure, describe some techniques fundamental to DNA analysis, and provide a brief overview of the clinical applications of this technology with respect to dentinogenesis imperfecta and oral field cancerization.
Todd
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Department of Oral and Maxillofacial Surgery, Harvard School of Dental Medicine, Boston, MA, USA. randy_todd@hms.harvard.edu
Donoff
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Kim
Y
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Wong
D T
DT
D015139:Blotting, Southern; D004247:DNA / Q000737:chemistry; D004262:DNA Restriction Enzymes / Q000737:chemistry; D003811:Dentinogenesis Imperfecta / Q000235:genetics; D008040:Genetic Linkage; D006801:Humans; D009062:Mouth Neoplasms / Q000235:genetics; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA / Q000379:methods*
D004247:DNA; D004262:DNA Restriction Enzymes