title: Preliminary study of the analysis of oligogalacturonic acids by electrospray ionization mass spectrometry.
abstract: During systematic studies of the behavior of oligogalacturonic acids (OGAs) under different conditions using electrospray ionization mass spectrometry (ESI-MS), cation adduction, fragmentation and non-covalent binding were found to be the three major problems that compromised the analysis of OGAs by ESI-MS. By adjusting solution components, capillary temperature and capillary voltage in the ESI source, an optimized condition was found that gave a clean and clear spectrum of trigalacturonic acid. A direct injection ESI-MS technique based on the use of aqueous acetonitrile and acetic acid and triethylamine (TEA) as modifiers has been applied to analyze a mixture including mono-, di- and trigalacturonic acids, which will facilitate further applications of ESI-MS in the analysis of mixtures of OGAs.
authors.affiliation: Department of Chemistry, National University of Singapore, 3 Science Drive 3, Republic of Singapore 117543.
authors.forename: L
authors.initials: L
authors.lastname: Zhu
authors.forename: H K
authors.initials: HK
authors.lastname: Lee
mesh_terms: D009844:Oligosaccharides / Q000032:analysis*; D010944:Plants / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D009844:Oligosaccharides; C412763:oligogalacturonic acid