[Determination of neohesperidin dihydrochalcone in foods].
An HPLC method was developed for the determination of neohesperidin dihydrochalcone (NHDC) in foods. A solid sample was extracted with methanol. The extract was evaporated and the residue was dissolved in methanol-water (2:8) mixture. In the case of fluid sample, the sample solution was prepared by dilution with methanol-water (2:8) mixture. The sample solution was cleaned up on a C18 cartridge. The cartridge was washed with water and methanol-water (3:7) mixture, and NHDC was eluted with methanol-water (7:3) mixture. NHDC in the eluate was separated on an ODS column and determined with a UV detector (282 nm). The identity of NHDC in foods was confirmed by means of HPLC with a photodiode-array detector. The recoveries of NHDC added to various kinds of foods were 75.2-134%. The determination limit of NHDC was 1 microgram/g.
Nakazato
M
M
Tama Branch Laboratory, Tokyo Metropolitan Research Laboratory of Public Health, 3-16-25, Shibazakicho, Tachikawa, Tokyo 190-0023, Japan.
Kobayashi
C
C
Yamajima
Y
Y
Kawano
M
M
Yasuda
K
K
D002599:Chalcone / Q000031:analogs & derivatives / Q000302:isolation & purification*; D047188:Chalcones; D002851:Chromatography, High Pressure Liquid; D005504:Food Analysis / Q000379:methods*; D006569:Hesperidin / Q000031:analogs & derivatives / Q000302:isolation & purification*; D013549:Sweetening Agents / Q000302:isolation & purification*
D047188:Chalcones; D013549:Sweetening Agents; C013613:neohesperidin dihydrochalcone; D002599:Chalcone; D006569:Hesperidin