Determination of ochratoxin A in pig liver-derived pâtés by high-performance liquid chromatography.
A solid phase extraction procedure followed by HPLC analysis with fluorescence detection has been developed for ochratoxin A (OTA) in pâtés derived from pig liver. After a previous extraction of OTA with 60% acetonitrile, all the samples were purified through C8 columns. The percentage recovery was 85.7% and the lower limits calculated for accurate detection and quantitation were 0.56 ng/g and 0.84 ng/g respectively. The HPLC procedure showed a good linearity in the interval of equivalent OTA concentrations of 0.84 to 3 ng/g. Values <10% were obtained for precision in HPLC determinations performed (n = 3) and (n = 9). Stability of calibration standards and samples during the analytical procedure was also demonstrated. This method was successfully applied to 38 pig-derived pâtés and three samples were found to be positive with OTA levels above the detection limit. The highest concentration (1.77 ng/g) has been found in a home-made pâté.
Jiménez
A M
AM
Department of Food Science and Toxicology, Faculty of Pharmacy, University of Navarra, Pamplona, Spain.
López de Cerain
A
A
Gonzalez-Peñas
E
E
Bello
J
J
D000818:Animals; D002273:Carcinogens / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005506:Food Contamination; D006801:Humans; D008099:Liver / Q000737:chemistry*; D008461:Meat Products / Q000032:analysis*; D009793:Ochratoxins / Q000032:analysis*; D013552:Swine
D002273:Carcinogens; D009793:Ochratoxins; C025589:ochratoxin A