Maintenance of TCR clonality in T cells expressing genes for two TCR heterodimers.
T cell receptor (TCR) allelic exclusion is believed to be primarily mediated by suppression of further recombination at the TCR locus after the expression of a functional TCR protein. Genetic allelic exclusion has been shown to be leaky for the beta chain and, more commonly, for the alpha chain. Here, we demonstrate an additional mechanism by which T cells can maintain monoclonality. T cells from double TCR transgenic mice express only one or the other of the two available TCRs at the cell surface. This "functional allelic exclusion" is apparently due to control of the TCR assembly process because these T cells express RNA and protein for all four transgenic TCR proteins. Lack of cell surface expression of the second TCR may be controlled by a failure to assemble the TCR heterodimer.
Sant'Angelo
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DB
Immunology Program, Memorial Sloan-Kettering Cancer Center, Weill Graduate School of Medical Sciences of Cornell University, New York, NY 10021, USA. d-sant'angelo@ski.mskcc.org
Cresswell
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Janeway
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CA
Denzin
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LK
D000483:Alleles; D019281:Dimerization; D005434:Flow Cytometry; D006801:Humans; D012333:RNA, Messenger / Q000032:analysis; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013601:T-Lymphocytes / Q000378:metabolism*; D019076:Transgenes
D012333:RNA, Messenger; D016693:Receptors, Antigen, T-Cell, alpha-beta