title: Age differences in immunohistochemical localizations of large proteoglycan, PG-M/versican, and small proteoglycan, decorin, in the dermis of rats.
abstract: Proteoglycans were localized immunohistochemically in the dermis of Donryu rats, using monoclonal antibodies raised against large proteoglycan (PG-M/versican) and small proteoglycan (decorin). The localizations of these proteoglycans in the dermis were compared between young rats (22-day old) and old ones (24 or 30 months of age), and distinct age differences were observed. In the young dermis, PG-M/versican was observed to be abundant in almost all fibroblastic cells (both cytoplasm and cell processes) whose cellularity was very rich compared with the dermis of old rats. Decorin was only faintly visible in the interstitial fibrous elements of young dermis. In the old dermis, however, decorin was distinctly detected on the fibrous elements, which were diffusely distributed as a fibrous network, and likewise PG-M/versican was visible in only a few fibrous elements which seemed to be the fine processes of fibroblastic cells. In the border layer between epidermis and dermis as well as the basal layer surrounding hair follicles, both large and small proteoglycans could be observed in old dermis. Since decorin, which was abundant in old dermis, has been found to have a growth inhibitory effect, it is conceivable that decorin may be one of the Cell Growth Inhibitory Factors in aging as proposed by Tauchi et al. [17, 18].
authors.affiliation: Institute for Medical Science of Aging, Aichi Medical University, 21 Karimata, Nagakute-cho, Aichi-gun, Aichi 480-1195, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Ito
authors.forename: J
authors.initials: J
authors.lastname: Takeuchi
authors.forename: K
authors.initials: K
authors.lastname: Yamamoto
authors.forename: Y
authors.initials: Y
authors.lastname: Hashizume
authors.forename: T
authors.initials: T
authors.lastname: Sato
authors.forename: H
authors.initials: H
authors.lastname: Tauchi
mesh_terms: D000375:Aging; D000818:Animals; D000911:Antibodies, Monoclonal; D011508:Chondroitin Sulfate Proteoglycans / Q000032:analysis*; D058575:Decorin; D020405:Dermis / Q000737:chemistry*; D004817:Epidermis / Q000737:chemistry; D016326:Extracellular Matrix Proteins; D005260:Female; D005347:Fibroblasts / Q000737:chemistry; D007150:Immunohistochemistry; D037181:Lectins, C-Type; D008297:Male; D011509:Proteoglycans / Q000032:analysis*; D051381:Rats; D053675:Versicans
chemical_list: D000911:Antibodies, Monoclonal; D011508:Chondroitin Sulfate Proteoglycans; C546880:Dcn protein, rat; D058575:Decorin; D016326:Extracellular Matrix Proteins; D037181:Lectins, C-Type; D011509:Proteoglycans; C508324:Vcan protein, rat; D053675:Versicans