Variant chromatin structure of the oriP region of Epstein-Barr virus and regulation of EBER1 expression by upstream sequences and oriP.
Most of the Epstein-Barr virus genome in latently infected cells is in a standard nucleosomal structure, but the region encompassing oriP and the Epstein-Barr virus-encoded small RNA (EBER) genes shows a distinctive pattern when digested with micrococcal nuclease. This pattern corresponds to a previously mapped nuclear matrix attachment region. Although the EBER genes are adjacent to oriP, there is only a two- to fourfold effect of oriP on EBER expression. However, sequences containing a consensus ATF site upstream of EBER1 are important for EBER1 expression.
Wensing
B
B
Ludwig Institute for Cancer Research, Imperial College School of Medicine, Norfolk Place, London W2 1PG, United Kingdom.
Stühler
A
A
Jenkins
P
P
Hollyoake
M
M
Karstegl
C E
CE
Farrell
P J
PJ
D002843:Chromatin / Q000737:chemistry*; D004261:DNA Replication; D004279:DNA, Viral / Q000378:metabolism; D015967:Gene Expression Regulation, Viral; D004854:Herpesvirus 4, Human / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012367:RNA, Viral / Q000235:genetics*; D014158:Transcription, Genetic
D002843:Chromatin; D004279:DNA, Viral; C099537:Epstein-Barr virus encoded RNA 1; D012367:RNA, Viral