Cloning and characterization of a chitosanase gene from the koji mold Aspergillus oryzae strain IAM 2660.
A genomic copy of the gene coding for chitosanase (csnA) was isolated from Aspergillus oryzae IAM 2660. A. oryzae csnA contains an open reading frame that encodes a polypeptide of 245 amino acids with a calculated molecular mass of 26,500 Da. The deduced amino acid sequence of A. oryzae csnA indicates extensive similarities to those of other fungal chitosanases.
Zhang
X Y
XY
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, Ueda, Nagano, Japan.
Dai
A L
AL
Kuroiwa
K
K
Kodaira
R
R
Nogawa
M
M
Shimosaka
M
M
Okazaki
M
M
D000595:Amino Acid Sequence; D001232:Aspergillus fumigatus / Q000201:enzymology / Q000235:genetics; D001236:Aspergillus oryzae / Q000201:enzymology* / Q000235:genetics*; D003001:Cloning, Molecular; D005670:Fusarium / Q000201:enzymology / Q000235:genetics; D015723:Gene Library; D006026:Glycoside Hydrolases / Q000235:genetics*; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013045:Species Specificity
D006026:Glycoside Hydrolases; C019868:chitosanase