title: Similar PAI-1 expression in visceral and subcutaneous fat of postmenopausal women.
abstract: Recent studies showed that intraabdominal visceral fat located in the mesenterium and omentum may significantly influence the circulating plasminogen activator inhibitor type 1 (PAI-1). To substantiate this link, we performed analysis of PAI-1 expression in human visceral and subcutaneous adipose tissues in peri- and postmenopausal women. The samples of both visceral and subcutaneous fat from 28 generally healthy women (aged 45-69 years) with a wide range of body mass index (BMI; 22.30-38.67 kg/m2), who underwent surgical operation due to benign ovary and uterine tumours, were obtained. In these samples, expression of mRNAs for PAI-1, tumour necrosis factor alpha (TNFalpha), acyl-CoA synthetase (ACS), and glucose transporter (GLUT-4) was analysed by relative quantitative RT PCR and correlated with plasma PAI-1 antigen. In addition, visceral fat area was measured with computer tomography. Both types of fat tissues contained similar quantities of PAI-1 mRNA, and there was no correlation between plasma PAI-1, measured both by antigen and activity, with either visceral or subcutaneous fat PAI-1 mRNA. Furthermore, there was no significant association between the expression of PAI-1 mRNA and TNFalpha mRNA in tested fat samples. PAI-1 mRNA in both fat tissues was significantly correlated with plasma levels of estradiol (positive correlation) and follicle-stimulating hormone (FSH; negative correlation). Finally, the expression of PAI-1 mRNA was negatively correlated with mRNA of ACS present in both fat tissues. In summary, this study directly indicates that PAI-1 mRNA is similarly expressed in both subcutaneous and visceral fat of peri- and postmenopausal women and its expression strongly depends upon lipid metabolism.
authors.affiliation: Polish Mother's Memorial Research Institute, Polish Academy of Sciences, Warsaw, Poland.
authors.forename: I
authors.initials: I
authors.lastname: Polac
authors.forename: A
authors.initials: A
authors.lastname: Cierniewska-Cieslak
authors.forename: G
authors.initials: G
authors.lastname: Stachowiak
authors.forename: T
authors.initials: T
authors.lastname: Pertynski
authors.forename: C S
authors.initials: CS
authors.lastname: Cierniewski
mesh_terms: D000273:Adipose Tissue / Q000737:chemistry*; D000368:Aged; D002979:Climacteric / Q000097:blood / Q000378:metabolism; D003066:Coenzyme A Ligases / Q000235:genetics; D005260:Female; D051275:Glucose Transporter Type 4; D006801:Humans; D050356:Lipid Metabolism; D008875:Middle Aged; D009004:Monosaccharide Transport Proteins / Q000235:genetics; D009124:Muscle Proteins; D017395:Plasminogen Activator Inhibitor 1 / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D017698:Postmenopause / Q000097:blood / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics; D014781:Viscera / Q000737:chemistry / Q000166:cytology
chemical_list: D051275:Glucose Transporter Type 4; D009004:Monosaccharide Transport Proteins; D009124:Muscle Proteins; D017395:Plasminogen Activator Inhibitor 1; D012333:RNA, Messenger; C495692:SLC2A4 protein, human; D014409:Tumor Necrosis Factor-alpha; D003066:Coenzyme A Ligases