[Concentrations of MMP-1, TIMP-1, MMP-1/TIMP-1 and I CTP complexes in follicular fluid as related to fertilization rate in women treated with in-vitro fertilization].
OBJECTIVE
Metalloproteinases and their inhibitors appear to control connective tissue remodelling during follicular rupture. The aim of the study was to establish if oocytes fertilisation rate after ovulation induction depends on the concentrations of MMP-1, its inhibitor TIMP-1, MMP-1/TIMP-1 complex and I CTP in follicular fluid (FF).


METHODS
FF were collected from 37 infertile patients undergoing ovulation induction using either short or long protocol. FF was obtained 36 hours after administration of hCG (Pregnyl). The level of MMP-1, TIMP-1, MMP-1/TIMP-1 complex were measured using ELISA kits and I CTP, E2, FSH, LH, using RIA assay kits.


RESULTS
Statistically significant (p < 0.05) difference was found in TIMP-1, E2, and FSH concentration, being higher in the group with more than 75% fertilisation rate: TIMP-1 728.8 + 100.1 ng/ml vs 666.3 + 94.5 ng/ml; E2 477.3 +/- 160.0 ng/ml vs 368.0 +/- 190.0 ng/ml and FSH 7.27 +/- 1.45 mIU/ml vs 6.24 +/- 1.6 mIU/ml.


CONCLUSIONS
Statistically significant increase in TIMP-1 concentration observed among patients with fertilisation rate above 75% indicates an important role of this substance in ovulation process.
Grabiec
M
M
Katedry i Kliniki Połoznictwa i Chorób Kobiecych AM w Bydgoszczy.
Szymański
W
W
Szymański
M
M
Jendryczka
J
J
Polak
G
G
Gogacz
M
M
Rechberger
T
T
D000328:Adult; D015415:Biomarkers / Q000032:analysis; D004958:Estradiol / Q000032:analysis; D005260:Female; D005307:Fertilization in Vitro; D005640:Follicle Stimulating Hormone / Q000032:analysis; D015571:Follicular Fluid / Q000737:chemistry*; D006801:Humans; D007247:Infertility, Female / Q000378:metabolism* / Q000628:therapy*; D020781:Matrix Metalloproteinase 1 / Q000032:analysis*; D010060:Ovulation / Q000502:physiology; D016305:Thymopentin / Q000031:analogs & derivatives / Q000032:analysis*; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000032:analysis*
D015415:Biomarkers; C106419:CTP-1 peptide; D019715:Tissue Inhibitor of Metalloproteinase-1; D004958:Estradiol; D005640:Follicle Stimulating Hormone; D020781:Matrix Metalloproteinase 1; D016305:Thymopentin