Equipotent Generation of Protective Antitumor Immunity by Various Methods of Dendritic Cell Loading With Whole Cell Tumor Antigens.
Multiple clinically applicable methods have been used to induce dendritic cells (DCs) to express whole cell tumor antigens, including pulsing DCs with tumor lysate, and mixing DCs with apoptotic or live tumor cells. Herein we demonstrate, using two different tumor systems, that these methods are equipotent inducers of systemic antitumor immunity. Furthermore, tumor lysate pulsed DC vaccines generate more potent antitumor immunity than immunization with irradiated tumor cells plus the classic adjuvant, Corynebacterium parvum.
Lambert
Laura A.
LA
Department of Surgery, Dartmouth Medical School and Norris Cotton Cancer Center, Lebanon, New Hampshire, U.S.A.
Gibson
Glen R.
GR
Maloney
Marybeth
M
Barth
Richard J.
RJ