title: Structure and dynamics of 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase.
abstract: Folates are essential for life. Unlike mammals, most microorganisms must synthesize folates de novo. 6-Hydroxymethyl-7,8-dihydropterin pyrophosphokinase (HPPK) catalyzes pyrophosphoryl transfer from ATP to 6-hydroxymethyl-7,8-dihydropterin (HP), the first reaction in folate pathway, and therefore, is an ideal target for developing novel antimicrobial agents. Because of its small size and high thermal stability, E. coli HPPK is also an excellent model enzyme for studying the mechanisms of enzymatic pyrophosphoryl transfer. We have determined the crystal structures of HPPK in the unligated form and in complex with HP, two Mg2+ ions, and AMPCPP (an ATP analog that inhibits the enzymatic reaction). Comparison of the two crystal structures reveals dramatic conformational changes of three flexible loops and many side chains and possible roles of the active site residues.
authors.affiliation: Department of Biochemistry, Michigan State University, East Lansing, MI, USA. yanh@pilot.msu.edu
authors.forename: H
authors.initials: H
authors.lastname: Yan
authors.forename: J
authors.initials: J
authors.lastname: Blaszczyk
authors.forename: B
authors.initials: B
authors.lastname: Xiao
authors.forename: G
authors.initials: G
authors.lastname: Shi
authors.forename: X
authors.initials: X
authors.lastname: Ji
mesh_terms: D000255:Adenosine Triphosphate / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D000595:Amino Acid Sequence; D001051:Apoenzymes / Q000737:chemistry; D001665:Binding Sites; D018360:Crystallography, X-Ray; D017854:Diphosphotransferases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D008274:Magnesium / Q000737:chemistry / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment
chemical_list: D001051:Apoenzymes; D000255:Adenosine Triphosphate; D017854:Diphosphotransferases; C019684:2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase; D008274:Magnesium; C002630:alpha,beta-methyleneadenosine 5'-triphosphate