Urinary excretion pattern of catecholestrogens in preovulatory LH surge during the 4-day estrous cycle of rats.
The formation of catecholestrogens represents a major pathway of estrogen metabolism and catecholestrogens are regarded as the main estrogen metabolite in non-pregnant state of various mammalian systems. In the present investigation, level of 2-hydroxyestrone, the major catecholestrogen excreted in rat urine, was measured by radioimmunoassay following acid hydrolysis and column chromatography of the 24-h urine samples of female Sprague Dawley non-pregnant rats during their 4-day estrous cycle. Urinary levels of estrone, estradiol and estriol were measured. Unlike the plasma level, urinary 2-hydroxyestrone showed a marked increase during the pre-ovulatory LH surge suggesting a plausible role of catecholestrogen in the mid-cycle elevation of the gonadotropin level in normal cycling rats.
Panda
S K
SK
Department of Chemistry, Maharaja Manindra Chandra College, Calcutta, India. drskpanda@hotmail.com
Chattoraj
S C
SC
D000818:Animals; D004031:Diestrus; D004958:Estradiol / Q000652:urine; D004964:Estriol / Q000652:urine; D002393:Estrogens, Catechol / Q000652:urine*; D004970:Estrone / Q000652:urine; D004971:Estrus / Q000502:physiology*; D005260:Female; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D006894:Hydroxyestrones / Q000097:blood / Q000652:urine; D007986:Luteinizing Hormone / Q000097:blood / Q000378:metabolism*; D008297:Male; D008686:Metestrus; D010060:Ovulation; D011359:Proestrus; D011817:Rabbits; D011863:Radioimmunoassay; D051381:Rats
D002393:Estrogens, Catechol; D006894:Hydroxyestrones; D004970:Estrone; D004958:Estradiol; D007986:Luteinizing Hormone; D004964:Estriol; C024980:2-hydroxyestrone