The use of 7-amino-actinomycin D in the analysis of Candida albicans phagocytosis and opsonization.
We describe the use of 7-amino-actinomycin D (7AAD) to measure phagocytosis and the opsonizing capacity of serum. Heat-inactivated Candida albicans was previously stained with 7AAD and incubated with resident peritoneal macrophages. The samples were analyzed by flow cytometry and phagocytic cells were identified by their bright red fluorescence. This is a rapid, reproducible and reliable one-step procedure and provides a means of evaluating low levels of phagocytosis.
Ortega
E
E
Microbiology Unit, Department of Health Sciences, Faculty of Experimental Sciences, University of Jaén, Paraje Las Lagunillas s/n, 23071 Jaén, Spain.
Algarra
I
I
Serrano
M J
MJ
Alvarez de Cienfuegos
G
G
Gaforio
J J
JJ
D000818:Animals; D002176:Candida albicans / Q000276:immunology*; D002478:Cells, Cultured; D003609:Dactinomycin / Q000031:analogs & derivatives / Q000737:chemistry*; D005434:Flow Cytometry / Q000379:methods; D005456:Fluorescent Dyes / Q000737:chemistry*; D017737:Macrophages, Peritoneal / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D009895:Opsonin Proteins / Q000032:analysis*; D010587:Phagocytosis
D005456:Fluorescent Dyes; D009895:Opsonin Proteins; D003609:Dactinomycin; C025942:7-aminoactinomycin D