DNA copy number changes in familial malignant mesothelioma.
Malignant mesothelioma (MM) is predominantly a sporadic malignancy linked to exposure to asbestos. Clustering of MM in families suggests genetic susceptibility as a contributing factor. We performed comparative genomic hybridization (CGH) analysis on tumor samples from members of a family with MM of the pleura and a history of parental cancer. Our specific aim was to find a recurrent copy number loss indicating the chromosomal area to which a gene underlying the development of MM could be assigned according to the Knudson two-hit hypothesis. We found losses at 1p, 6q, 9p, 13q, and 14q. The copy number changes were very similar to those reported in sporadic cases. Our findings and results from sporadic cases highlight the importance of cloning the genes in the loss sites at 1p, 6q, 14q, and 22q.
Ascoli
V
V
Department of Experimental Medicine and Pathology, La Sapienza University, Viale Regina Elena 324, 00161 Rome, Italy. valeria.ascoli@uniroma1.it
Aalto
Y
Y
Carnovale-Scalzo
C
C
Nardi
F
F
Falzetti
D
D
Mecucci
C
C
Knuutila
S
S
D000368:Aged; D002869:Chromosome Aberrations / Q000235:genetics*; D004273:DNA, Neoplasm / Q000032:analysis*; D005260:Female; D018628:Gene Dosage; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D008297:Male; D008654:Mesothelioma / Q000235:genetics* / Q000473:pathology; D008875:Middle Aged; D009693:Nucleic Acid Hybridization; D010375:Pedigree; D010997:Pleural Neoplasms / Q000235:genetics* / Q000473:pathology; D017384:Sequence Deletion
D004273:DNA, Neoplasm