title: A multiprotein complex that interacts with RNA polymerase II elongator.
abstract: A three-subunit Hap complex that interacts with the RNA polymerase II Elongator was isolated from yeast. Deletions of genes for two Hap subunits, HAP1 and HAP3, confer pGKL killer-insensitive and weak Elongator phenotypes. Preferential interaction of the Hap complex with free rather than RNA polymerase II-associated Elongator suggests a role in the regulation of Elongator activity.
authors.affiliation: Department of Structural Biology, Stanford University School of Medicine, Stanford, California 94305-5400, USA. yli@tularik.com
authors.forename: Y
authors.initials: Y
authors.lastname: Li
authors.forename: Y
authors.initials: Y
authors.lastname: Takagi
authors.forename: Y
authors.initials: Y
authors.lastname: Jiang
authors.forename: M
authors.initials: M
authors.lastname: Tokunaga
authors.forename: H
authors.initials: H
authors.lastname: Erdjument-Bromage
authors.forename: P
authors.initials: P
authors.lastname: Tempst
authors.forename: R D
authors.initials: RD
authors.lastname: Kornberg
mesh_terms: D000595:Amino Acid Sequence; D023081:CCAAT-Binding Factor / Q000235:genetics; D019757:Carbon-Oxygen Lyases / Q000235:genetics; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000235:genetics; D008954:Models, Biological; D008969:Molecular Sequence Data; D009154:Mutation; D010641:Phenotype; D010766:Phosphorylation; D011233:Precipitin Tests; D011485:Protein Binding; D012319:RNA Polymerase II / Q000737:chemistry* / Q000302:isolation & purification*; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D029701:Saccharomyces cerevisiae Proteins; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000235:genetics
chemical_list: D023081:CCAAT-Binding Factor; D005656:Fungal Proteins; C490431:HAP3 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D012319:RNA Polymerase II; D019757:Carbon-Oxygen Lyases; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase