Cell surface human alpha-L-fucosidase.
The acid alpha-L-fucosidase is usually found as a soluble component of lysosomes where fucoglycoconjugates are degraded. In the present investigation, we have demonstrated the existence of a cell surface protein with enzymatic alpha-L-fucosidase activity that crossreacts specifically with a rabbit anti-(alpha-L-fucosidase) Ig. By different approaches, this alpha-L-fucosidase, which represents 10-20% of the total cellular fucosidase activity, was detected in all the tested human cells (hemopoietic, epithelial, mesenchymal). Two bands of approximately 43-49 kDa were observed, although theoretical data support the possibility of having the same genetic origin that the known 50 to 55-kDa Mr alpha-L-fucosidase. We speculate about an alternative traffic pathway for the plasma membrane alpha-L-fucosidase to work on the rapid turnover of glycoproteins.
Cordero
O J
OJ
Department of Biochemistry and Molecular Biology, University of Santiago de Compostela, Faculty of Biology, Santiago de Compostela, Spain. bnojcord@usc.es
Merino
A
A
Páez de la Cadena
M
M
Bugía
B
B
Nogueira
M
M
Viñuela
J E
JE
Martínez-Zorzano
V S
VS
de Carlos
A
A
Rodríguez-Berrocal
F J
FJ
D000818:Animals; D000906:Antibodies / Q000276:immunology; D000918:Antibody Specificity; D015153:Blotting, Western; D002458:Cell Fractionation; D002460:Cell Line; D002462:Cell Membrane / Q000201:enzymology*; D003432:Cross-Linking Reagents; D004591:Electrophoresis, Polyacrylamide Gel; D004847:Epithelial Cells / Q000201:enzymology; D005434:Flow Cytometry; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D008214:Lymphocytes / Q000201:enzymology; D009000:Monocytes / Q000201:enzymology; D009504:Neutrophils / Q000201:enzymology; D011233:Precipitin Tests; D005644:alpha-L-Fucosidase / Q000032:analysis* / Q000276:immunology / Q000378:metabolism
D000906:Antibodies; D003432:Cross-Linking Reagents; D005644:alpha-L-Fucosidase