title: Shikonin production by p-fluorophenylalanine resistant cells of Lithospermum erythrorhizon.
abstract: Studies were conducted with a BK-39 callus culture of Lithospermum erythrorhizon, which produced seven shikonin derivatives (acetylshikonin, propionylshikonin, isobutyrylshikonin, beta,beta-dimethylacrylshikonin, isovalerylshikonin, beta-hydroxyisovalerylshikonin and alpha-methyl-n-butyrylshikonin). A selection of cell aggregates of BK-39 culture on a medium containing p-fluorophenylalanine (PFP) yields a cell line possessing a higher resistance to the inhibitor than the initial culture. Selected BK-39F cultures produced almost the same profile of shikonin naphthoquinones as the initial culture. The shikonin derivative content of PFP-resistant culture was approximately two times higher than that of the control, reaching 12.6% of DW cell biomass.
authors.affiliation: The Institute of Biology and Soil Science of the Far East Branch of Russian Academy of Sciences, 690022, Vladivostok, Russia. biotech@eastnet.febras.ru
authors.forename: V P
authors.initials: VP
authors.lastname: Bulgakov
authors.forename: M M
authors.initials: MM
authors.lastname: Kozyrenko
authors.forename: S A
authors.initials: SA
authors.lastname: Fedoreyev
authors.forename: N P
authors.initials: NP
authors.lastname: Mischenko
authors.forename: V A
authors.initials: VA
authors.lastname: Denisenko
authors.forename: L V
authors.initials: LV
authors.lastname: Zvereva
authors.forename: T V
authors.initials: TV
authors.lastname: Pokushalova
authors.forename: Y N
authors.initials: YN
authors.lastname: Zhuravlev
mesh_terms: D002478:Cells, Cultured / Q000187:drug effects; D006801:Humans; D019684:Magnoliopsida; D009285:Naphthoquinones / Q000378:metabolism*; D018517:Plant Roots; D010946:Plants, Medicinal; D010135:p-Fluorophenylalanine / Q000494:pharmacology*
chemical_list: D009285:Naphthoquinones; C016101:shikonin; D010135:p-Fluorophenylalanine