title: Cerebral protection by hypoxic preconditioning in a murine model of focal ischemia-reperfusion.
abstract: Sublethal periods of hypoxia or ischemia can induce adaptive mechanisms to protect against subsequent lethal ischemic insults in a process known as ischemic preconditioning. In the present study, we developed a murine model of cerebral preconditioning using several common strains of adult mice. Animals were exposed to sublethal hypoxia (11% oxygen for 2 h) 48 h prior to a 90 min period of transient focal middle cerebral artery occlusion, induced by an intraluminal filament; injury was assessed 24 h later by TTC staining. Infarct volume in hypoxia-preconditioned animals was reduced 46%, 58%, and 64% in C57Bl/6, 129SvEv, and Swiss-Webster ND4 mice relative to their respective untreated controls. This non-invasive murine model of ischemic tolerance should be useful for elucidating the molecular basis of this protection using transgenic and knockout mice.
authors.affiliation: Department of Neurosurgery, Washington University School of Medicine, St. Louis, MO 63110, USA.
authors.forename: B A
authors.initials: BA
authors.lastname: Miller
authors.forename: R S
authors.initials: RS
authors.lastname: Perez
authors.forename: A R
authors.initials: AR
authors.lastname: Shah
authors.forename: E R
authors.initials: ER
authors.lastname: Gonzales
authors.forename: T S
authors.initials: TS
authors.lastname: Park
authors.forename: J M
authors.initials: JM
authors.lastname: Gidday
mesh_terms: D000818:Animals; D001921:Brain / Q000503:physiopathology*; D002545:Brain Ischemia / Q000503:physiopathology*; D002544:Cerebral Infarction / Q000473:pathology; D000860:Hypoxia / Q000503:physiopathology; D019194:Ischemic Preconditioning / Q000379:methods; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D015427:Reperfusion Injury / Q000503:physiopathology*