Activated protein C and inflammation in human myocardium after heart surgery.
To assess possible interactions between inflammation and activation of the anticoagulant protein C system during post-ischemic reperfusion protein C, APC, neutrophil L-selectin expression and myocardial myeloperoxidase activity (MPO) were measured in 19 patients undergoing cardiopulmonary bypass. After reperfusion for 10 min, APC to protein C ratio (APC/PC) increased from pre-reperfusion value 1.43 +/- 0.12 (mean +/- SEM) to 2.25 +/- 0.29, p = 0.015. Negative correlations were observed between APC/PC and MPO activity (Spearman r -0.64, p = 0.007) and APC/PC and neutrophil L-selectin expression (r = -0.7, p = 0.007, demonstrating that post-ishemic protein C activation was associated with decreased neutrophil tissue sequestration. Thus, physiological protein C activation may be involved in regulation of the inflammatory injury during reperfusion of human ischemic coronary circulation.
Petäjä
J
J
Hospital for Children and Adolescents, University of Helsinki, Helsinki, Finland. jari.petaja@dlc.fi
Pesonen
E
E
Fernández
J A
JA
Griffin
J H
JH
Repo
H
H
Jansson
S E
SE
Vento
A E
AE
Rämö
O J
OJ
D000328:Adult; D000368:Aged; D002315:Cardiopulmonary Bypass / Q000009:adverse effects*; D004789:Enzyme Activation; D006324:Heart Arrest, Induced / Q000009:adverse effects; D006801:Humans; D019041:L-Selectin / Q000032:analysis; D008297:Male; D008875:Middle Aged; D015428:Myocardial Reperfusion Injury / Q000097:blood* / Q000209:etiology; D009205:Myocarditis / Q000209:etiology*; D009504:Neutrophils / Q000201:enzymology; D009195:Peroxidase / Q000032:analysis; D011486:Protein C / Q000032:analysis*
D011486:Protein C; D019041:L-Selectin; D009195:Peroxidase