title: Adherence of Shigella dysenteriae 1 to human colonic mucin.
abstract: The pathogenic potential of Shigella is correlated with the ability of the organism to invade and multiply within the cells of colonic epithelium. Although invasion is the ultimate event, a preceding step is adherence. Shigella dysenteriae 1 preferentially adhered to colonic mucin and not to small intestinal mucin. The pathogen showed a very strong adherence pattern to human colonic mucin when compared with guinea pig and rat mucin. The adherence pattern of S. dysenteriae 1 was not altered on preincubation with monosaccharides present in mucins, suggesting that the receptor for the pathogen is not a simple sugar. Binding of S. dysenteriae 1 to human colonic mucin was not by weak hydrophobic forces. The bacterium also adhered to glycolipids, emphasizing the role of glycoconjugates as receptors for S. dysenteriae 1.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Madras, Guindy Campus, Chennai-600 025, India.
authors.forename: P S
authors.initials: PS
authors.lastname: Sudha
authors.forename: H
authors.initials: H
authors.lastname: Devaraj
authors.forename: N
authors.initials: N
authors.lastname: Devaraj
mesh_terms: D018829:Adhesins, Bacterial / Q000378:metabolism; D000818:Animals; D001422:Bacterial Adhesion; D001665:Binding Sites; D002460:Cell Line; D002850:Chromatography, Gel / Q000379:methods; D003106:Colon / Q000382:microbiology*; D004846:Epithelial Attachment; D006168:Guinea Pigs; D006801:Humans; D066298:In Vitro Techniques; D009077:Mucins / Q000378:metabolism*; D051381:Rats; D011971:Receptors, Immunologic / Q000378:metabolism; D012762:Shigella dysenteriae / Q000378:metabolism* / Q000472:pathogenicity*
chemical_list: D018829:Adhesins, Bacterial; D009077:Mucins; D011971:Receptors, Immunologic; C047105:bacterial adhesin receptor