title: Effect of mirabilitum in formularization: change of prolyl endopeptidase inhibitory activity and of constituents using the preparation method of Tokaku-joki-to.
abstract: To clarify the effect of Mirabilitum in formularization, change of prolyl endopeptidase inhibitory activity and of constituents using the preparation method of a Kampo formula Tokaku-joki-to ([Japanese characters: see text], Persia and Rhubarb combination) was examined by the liquid chromatography-mass spectroscopy (LC-MS) method. Mirabilitum under boiling condition caused qualitative and quantitative change of the constituents through hydrolysis which caused a change of its activity. This was considered to be the main reason the classical Chinese medical book "Shang han lun ([Japanese characters: see text])" specified that Mirabilitum should be added at a later stage of decoction.
authors.affiliation: Institute of Natural Medicine, Toyama Medical and Pharmaceutical University, Sugitani, Japan.
authors.forename: W
authors.initials: W
authors.lastname: Fan
authors.forename: Y
authors.initials: Y
authors.lastname: Tezuka
authors.forename: S
authors.initials: S
authors.lastname: Kadota
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D013058:Mass Spectrometry; D010946:Plants, Medicinal / Q000737:chemistry*; D000085822:Prolyl Oligopeptidases; D011480:Protease Inhibitors / Q000494:pharmacology*; D012015:Reference Standards; D012250:Rheum / Q000737:chemistry*; D012697:Serine Endopeptidases / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet; D013431:Sulfates / Q000494:pharmacology*
chemical_list: D004365:Drugs, Chinese Herbal; D011480:Protease Inhibitors; D013431:Sulfates; C112001:tokaku-joki-to; C012036:sodium sulfate; D012697:Serine Endopeptidases; D000085822:Prolyl Oligopeptidases