title: The effect of shaking regime on the rate and extent of enzymatic hydrolysis of cellulose.
abstract: In an attempt to elucidate the effect of mixing on the rate and extent of enzymatic hydrolysis of cellulosic substrates, alpha-cellulose was hydrolysed using a commercial cellulase preparation at varying levels of substrate concentration (2.5,5 and 7.5% (w/v)) and by using three shaking regimes: continuous at low-speed (25 rpm), continuous at high-speed (150 rpm) and an intermittent regime comprised of high and low-speed shaking intervals. The continuous, high-speed shaking produced the highest conversion yields, whereas the intermittent and low-speed shaking regimes resulted in lower conversions. After 72 h, at all shaking regimes (150 rpm,25 rpm and intermittent), using a low substrate concentration (2.5%) produced conversion yields (82,79 and 80%) higher than those obtained at high (7.5%) substrate concentration (68,63 and 68%). As the substrate concentration increased, the conversion yields at intermittent shaking gradually approached those resulting from high-speed shaking. Thus, it appears that intermittent shaking could be a beneficial process option as it can reduce the mixing energy requirements while producing reasonably high conversion yields.
authors.affiliation: Department of Chemical Engineering 1, Lund University, Lund, Sweden.
authors.forename: H
authors.initials: H
authors.lastname: Ingesson
authors.forename: G
authors.initials: G
authors.lastname: Zacchi
authors.forename: B
authors.initials: B
authors.lastname: Yang
authors.forename: A R
authors.initials: AR
authors.lastname: Esteghlalian
authors.forename: J N
authors.initials: JN
authors.lastname: Saddler
mesh_terms: D001709:Biotechnology / Q000379:methods*; D002480:Cellulase / Q000737:chemistry / Q000378:metabolism*; D002482:Cellulose / Q000378:metabolism*; D006868:Hydrolysis
chemical_list: D002482:Cellulose; D002480:Cellulase