Replacement of the canine inferior vena cava with a seeded graft.
The patency and microscopic findings of gelatin-coated Dacron grafts seeded with endothelial cells and implanted into the inferior vena cava of dogs was evaluated. A total of 25 mongrel dogs were divided into four experimental groups according to whether or not an antiplatelet agent was administered and a seeded or nonseeded graft was implanted. In the groups not given antiplatelet therapy that were implanted with a nonseeded graft, occlusion of the graft occurred soon after surgery. In contrast, in the majority of animals implanted with a seeded graft and given antiplatelet therapy, graft patency was evident for up to 4 weeks postoperatively. In the latter animals, scanning electron microscopy 4 weeks after surgery showed the formation of confluent endothelial-like cells within 5 mm from the anastomosis, as well as insular endothelial-like cells in the central part of the graft. The simple centrifugal seeding method enables endothelial-like cells to adhere to grafts, suggesting that it may be useful in venous replacement.
Shimizu
M
M
First Department of Surgery, Institute of Neurological Diseases, Hirosaki University School of Medicine, 5 Zaifucho, Hirosaki 036-8203, Japan.
Suzuki
S
S
Takaya
S
S
Satoh
K
K
D000818:Animals; D002448:Cell Adhesion; D002498:Centrifugation; D004195:Disease Models, Animal; D004285:Dogs; D004727:Endothelium / Q000166:cytology*; D005780:Gelatin; D006083:Graft Occlusion, Vascular / Q000503:physiopathology / Q000517:prevention & control*; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D011093:Polyethylene Terephthalates; D019736:Prostheses and Implants; D019919:Prosthesis Implantation / Q000379:methods; D014656:Vascular Surgical Procedures / Q000379:methods*; D014682:Vena Cava, Inferior / Q000473:pathology / Q000601:surgery*
D010975:Platelet Aggregation Inhibitors; D011093:Polyethylene Terephthalates; D005780:Gelatin