[Dynamic of immune response in primary cytomegalovirus infection and after reactivation of cytomegalovirus in patients with organ allotransplantation].
A total of 44 serum specimens from 7 patients with kidneys or liver transplanted from donors who had antibodies (Ab) to human cytomegalovirus (CMV) were studied. In 4 recipients anti-CMV Ab were found before transplantation and in 3 others they were not detected. It was shown by EIA that IgM and IgG anti-CMV appeared in the sera of primarily infected patients after 1-2 weeks and their titers were 5-10 times lower than in patients with reactivated CMV infection. Immunoblotting of Ab to individual CMV proteins showed a narrower spectrum of Ab during the initial period of primary CMV infection in comparison with the same period of reactivation and delayed production of AB to conformation-dependent determinants. Hence, analysis of anti-CMV Ab during the first 4-6 weeks after organ transplantation by EIA and immunoblotting differentiates primary CMV infection from its reactivation by Ab titers and spectrum. These parameters vary in some patients.
Pustowoit
B
B
Herrmann
F
F
Makarova
N E
NE
Kushch
A A
AA
D000914:Antibodies, Viral / Q000276:immunology*; D000956:Antigens, Viral / Q000737:chemistry / Q000276:immunology; D003587:Cytomegalovirus / Q000276:immunology*; D003586:Cytomegalovirus Infections / Q000209:etiology / Q000276:immunology*; D006801:Humans; D007109:Immunity; D016056:Immunodominant Epitopes; D009894:Opportunistic Infections / Q000276:immunology; D016377:Organ Transplantation / Q000009:adverse effects; D011487:Protein Conformation; D013329:Structure-Activity Relationship; D014181:Transplantation Immunology; D014184:Transplantation, Homologous
D000914:Antibodies, Viral; D000956:Antigens, Viral; D016056:Immunodominant Epitopes