Chromatographic (GC-MS, HPLC) and virological evaluations of Salvia sclarea infected by BBWV-I.
Salvia sclarea cultivated at the Herb Garden of Casola-Valsenio (Emilia-Romagna region, Italy) has been found for the first time naturally infected by broad bean wilt fabavirus, serotype I (BBWV-I). Symptomatic plants showed malformed leaves, with chlorotic mosaic followed by yellowing and stunting. BBWV-I was identified by applying virological tests: mechanical inoculations on herbaceous plants, electron microscopy, DAS-ELISA and PAS-ELISA. The essential oil obtained from BBWV-infected material corresponded to 2/3 the quantity of that from healthy material. The GC-MS and HPLC analyses of these oils afforded a comparative analytical profile of the two plant materials attributed to BBWV-I infection. The oils from infected materials showed higher percentages of sesquiterpene hydrocarbons (e.g. germacrene D and beta-caryophyllene), monoterpene alcohols (e.g. alpha-terpineol) and diterpenoids (mainly sclareol). In contrast, lower levels of monoterpene hydrocarbons (e.g. myrcene, limonene and the two ocimene isomers) and the principal components (linalyl acetate and linalool) were observed.
Hudaib
M
M
Dipartimento di Scienze Farmaceutiche, University of Bologna, Via Belmeloro 6, 40126 Bologna, Italy.
Bellardi
M G
MG
Rubies-Autonell
C
C
Fiori
J
J
Cavrini
V
V
D002851:Chromatography, High Pressure Liquid; D019181:Fabavirus / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D007558:Italy; D013058:Mass Spectrometry; D009822:Oils, Volatile / Q000737:chemistry; D010935:Plant Diseases / Q000821:virology*; D010942:Plant Viruses / Q000737:chemistry*; D027543:Salvia officinalis / Q000737:chemistry* / Q000821:virology*
D009822:Oils, Volatile