title: Differential expression of CD44 isoforms during liver regeneration in rats.
abstract: BACKGROUND
  CD44 is a transmembrane glycoprotein known to bind hyaluronic acid (HA). This molecule is a multifunctional cell surface glycoprotein involved in lymphocyte homing and activation, tumor growth and metastasis. We have investigated the qualitative modification of CD44 in the regenerating liver as a model for studying cellular proliferation in vivo. Molecules involved in cell adhesion and the extracellular matrix (ECM), which influence differentiation, growth, cell-cell interactions and cellular polarity, play an important role in the liver regeneration. We studied the modulation of CD44 gene expression and its post-transcriptional modifications, analyzing the expression of different isoforms containing exon v6 in the regenerating liver, in sham operated liver and in the hepatoma cells H-35.
  
  
  METHODS
  The expression of CD44 and CD44v6 were analyzed in RNA extracted from regenerating liver at different times after partial hepatectomy (PH), and H-35 hepatoma cells by Northern blot, RT-PCR and Southern blot, and in protein extracts from regenerating liver by Western blot. H-35 hepatoma cells were assayed with the antibody cross-linked technique with CD44 antibodies.
  
  
  RESULTS
  The standard CD44 form is expressed in regenerating liver and its levels were not modified following PH. However, our analysis revealed CD44 isoforms containing v6 in the first hours after PH as well as in the H-35 hepatoma cell line. H-35 cells treated with cross-linked anti-CD44 antibodies or HA show an increased rate of incorporation of [3H]thymidine (30 and 25%, respectively) with respect to the control.
  
  
  CONCLUSIONS
  These findings suggest that CD44 may play a role in the proliferation of residual hepatocytes following PH.
authors.affiliation: Dipartimento di Scienze Biochimiche e di Biotecnologie Molecolari, Università di Perugia, Policlinico Monteluce, Italy.
authors.forename: M A
authors.initials: MA
authors.lastname: Della Fazia
authors.forename: V
authors.initials: V
authors.lastname: Pettirossi
authors.forename: E
authors.initials: E
authors.lastname: Ayroldi
authors.forename: C
authors.initials: C
authors.lastname: Riccardi
authors.forename: M V
authors.initials: MV
authors.lastname: Magni
authors.forename: G
authors.initials: G
authors.lastname: Servillo
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D018960:Hyaluronan Receptors / Q000276:immunology / Q000378:metabolism*; D008099:Liver / Q000166:cytology; D008115:Liver Regeneration / Q000502:physiology*; D008297:Male; D020033:Protein Isoforms / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D014407:Tumor Cells, Cultured
chemical_list: D000911:Antibodies, Monoclonal; D018960:Hyaluronan Receptors; D020033:Protein Isoforms