End-labeling of long DNA fragments with biotin and detection of DNA immobilized on magnetic beads.
To immobilize DNA fragments onto magnetic beads coated with streptavidin for isolation purpose, it is important to label one biotin molecule at one terminus of DNA fragment. After failure to label long DNA with biotin by PCR and filling-in reaction, a 9.2 kb DNA was labeled with biotin by a modified ligation strategy. A simple method is also reported to detect the quantity and integrity of DNA immobilized on the magnetic beads.
Xu
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H
National Laboratory of Medical Molecular Biology, Institute of Basic Medical Science, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, P.R. China.
Zhang
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Liu
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D
Liang
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CC
D001483:Base Sequence; D001709:Biotechnology; D001710:Biotin; D004247:DNA / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D017931:DNA Primers / Q000235:genetics; D005914:Globins / Q000235:genetics; D006801:Humans; D008280:Magnetics; D008863:Microspheres; D016133:Polymerase Chain Reaction; D019809:Streptavidin
D017931:DNA Primers; D001710:Biotin; D005914:Globins; D004247:DNA; D019809:Streptavidin