title: Desulfurization of benzothiophene by the Gram-negative bacterium, Sinorhizobium sp. KT55.
abstract: Sinorhizobium sp. KT55 was the first Gram-negative isolate to be capable of utilizing benzothiophene as the sole source of sulfur. By GC-MS analysis of metabolites of benzothiophene by this strain, benzothiophene sulfone, benzo[e][1,2]oxathiin S-oxide and o-hydroxystyrene were detected, suggesting that the benzothiophene desulfurization pathway of this strain is benzothiophene-->benzothiophene sulfoxide-->benzothiophene sulfone-->benzo[e][1,2]oxathiin S-oxide-->o-hydroxystyrene. Desulfurization activity of this strain was significantly repressed by methionine, cysteine, sulfate, dimethyl sulfoxide, and Casamino acids.
authors.affiliation: Tsukuba Branch of Bio-Refining Process Laboratory, Advanced Technology and Research Institute, Japan Petroleum Energy Center, 1-1 Higashi Tsukuba, Ibaraki 305-8566, Japan. spweek@nibh.go.jp
authors.forename: Y
authors.initials: Y
authors.lastname: Tanaka
authors.forename: T
authors.initials: T
authors.lastname: Onaka
authors.forename: T
authors.initials: T
authors.lastname: Matsui
authors.forename: K
authors.initials: K
authors.lastname: Maruhashi
authors.forename: R
authors.initials: R
authors.lastname: Kurane
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D001673:Biodegradation, Environmental; D003470:Culture Media; D004121:Dimethyl Sulfoxide / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D020579:Sinorhizobium / Q000254:growth & development / Q000378:metabolism*; D013455:Sulfur / Q000378:metabolism; D013876:Thiophenes / Q000378:metabolism*
chemical_list: D000596:Amino Acids; D003470:Culture Media; D013876:Thiophenes; C088015:benzothiophene; D013455:Sulfur; D004121:Dimethyl Sulfoxide