Light-dependent generation of reactive oxygen species in cell culture media.
Cell culture media (RPMI 1640, Dulbecco's Minimal Essential Medium and yeast extract-peptone-glucose medium) were found to oxidize dichlorodihydrofluorescein diacetate and dihydrorhodamine 123, and to generate spin adduct of 5,5'-dimethyl-1-pyrroline N-oxide, which indicates formation of reactive oxygen species (ROS). The production of ROS was light dependent. The main component of the media responsible for the generation of ROS was riboflavin, but tryptophan, tyrosine, pyridoxine, and folic acid enhanced the effect of riboflavin. These observations point to exposure of cells to ROS under in vitro culture conditions.
Grzelak
A
A
Department of Molecular Biophysics, University of Lódz, Lódz, Poland.
Rychlik
B
B
Bartosz
G
G
D001386:Azides / Q000494:pharmacology; D003470:Culture Media / Q000737:chemistry / Q000528:radiation effects*; D003497:Cyclic N-Oxides; D004578:Electron Spin Resonance Spectroscopy; D005452:Fluoresceins / Q000528:radiation effects; D005456:Fluorescent Dyes / Q000528:radiation effects; D005492:Folic Acid / Q000528:radiation effects; D006868:Hydrolysis; D017665:Hydroxyl Radical; D008027:Light; D010084:Oxidation-Reduction; D010100:Oxygen; D010637:Phenolsulfonphthalein / Q000528:radiation effects; D010777:Photochemistry; D010879:Piperazines / Q000494:pharmacology; D011736:Pyridoxine / Q000528:radiation effects; D017382:Reactive Oxygen Species; D012235:Rhodamines / Q000528:radiation effects; D012256:Riboflavin / Q000528:radiation effects; D026082:Singlet Oxygen; D013113:Spin Labels; D013481:Superoxides / Q000032:analysis; D014364:Tryptophan / Q000528:radiation effects; D014443:Tyrosine / Q000528:radiation effects
C110400:2',7'-dichlorodihydrofluorescein diacetate; D001386:Azides; D003470:Culture Media; D003497:Cyclic N-Oxides; D005452:Fluoresceins; D005456:Fluorescent Dyes; D010879:Piperazines; D017382:Reactive Oxygen Species; D012235:Rhodamines; D013113:Spin Labels; C058319:dihydrorhodamine 123; D013481:Superoxides; D026082:Singlet Oxygen; D017665:Hydroxyl Radical; D014443:Tyrosine; C017245:5,5-dimethyl-1-pyrroline-1-oxide; D014364:Tryptophan; D005492:Folic Acid; D010637:Phenolsulfonphthalein; D011736:Pyridoxine; D010100:Oxygen; D012256:Riboflavin; C007306:triethylenediamine