Comparison of the genome of ovine adenovirus types 1 through 5 by restriction enzyme analysis and DNA hybridisation.
The DNA of the prototype strains of ovine adenovirus (OAdV) 1 through 5 was analysed by restriction enzyme (RE) digestion. The RE patterns generated by HindIII and PstI enzymes were characteristic of the examined strains. OAdV-2 and 3 resembled each other the most, and their EcoRI and HindIII patterns seemed to be identical. Considering the number of comigrating fragments, serotypes OAdV-2, 3, 4 and 5 looked more closely related to each other than to OAdV-1. This finding was strengthened by Southern blot hybridisations probed with random HindIII clones of OAdV-3. The estimated genome size of the examined OAdV types ranged between 31.9 and 32.8 kilobase pairs. The results supported the new genus classification of OAdVs.
Benkö
M
M
Veterinary Medical Research Institute, Hungarian Academy of Sciences, H-1581 Budapest, P.O. Box 18, Hungary. benko@novell.vmri.hu
D000818:Animals; D004279:DNA, Viral / Q000145:classification / Q000235:genetics*; D016679:Genome, Viral; D017921:Mastadenovirus / Q000145:classification / Q000235:genetics*; D015183:Restriction Mapping / Q000379:methods; D012703:Serotyping; D012756:Sheep
D004279:DNA, Viral