title: Vitamin E protects against iron-hexachlorobenzene induced porphyria and formation of 8-hydroxydeoxyguanosine in the liver of C57BL/10ScSn mice.
abstract: The effect of vitamin E treatment on total porphyrin content, lipid peroxidation (LOOH) and 8-hydroxydeoxyguanosine (8-OHdG) was studied in the livers of C57BL/10ScSn mice following hexachlorobenzene (HCB) and iron treatment. HCB was administered i.p. (totalling 300 mg/kg) twice, with 1 week interval. Three days after the first HCB injection iron-dextran was given i.p. (500 mg Fe per kg). Vitamin E was administered weekly (20 mg/kg) by s.c. injection. Both total hepatic porphyrin and LOOH levels were significantly (P<0.001) increased in the HCB-iron treated group as compared with the control group. Mice treated additionally with vitamin E had significant (P<0.001) lower levels as compared with the HCB-iron group. Similarly, the levels of 8-OHdG were significantly (P<0.001) increased above controls after HCB-iron treatment and this increase was reduced after co-treatment with vitamin E (P<0.02). The data support the hypothesis that the mechanism of hepatic porphyrinogenicity of HCB with iron overload is an oxidative free radical process.
authors.affiliation: Second Department of Medicine, Semmelweis University Medical School, Szentkriralyi u.46 1088, Budapest, Hungary.
authors.lastname: Horváth ME
authors.forename: S P
authors.initials: SP
authors.lastname: Faux
authors.forename: A G
authors.initials: AG
authors.lastname: Smith
authors.forename: A
authors.initials: A
authors.lastname: Blázovics
authors.forename: M
authors.initials: M
authors.lastname: van der Looij
authors.forename: J
authors.initials: J
authors.lastname: Fehér
authors.forename: K H
authors.initials: KH
authors.lastname: Cheeseman
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000818:Animals; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000378:metabolism*; D006581:Hexachlorobenzene / Q000633:toxicity*; D007501:Iron / Q000378:metabolism / Q000633:toxicity*; D008054:Lipid Peroxides / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D017094:Porphyrias, Hepatic / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D011166:Porphyrins / Q000378:metabolism; D014810:Vitamin E / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D008054:Lipid Peroxides; D011166:Porphyrins; D014810:Vitamin E; D006581:Hexachlorobenzene; D000080242:8-Hydroxy-2'-Deoxyguanosine; D007501:Iron; D003849:Deoxyguanosine