title: Hormone-induced nucleosome positioning in the MMTV promoter is reversible.
abstract: The mouse mammary tumor virus (MMTV) promoter is induced by glucocorticoid hormone via the glucocorticoid receptor (GR). The hormone-triggered effects on MMTV transcription and chromatin structure were studied in Xenopus oocytes. We previously showed that the nucleosomes organizing the MMTV promoter became translationally positioned upon hormone induction. A single GR-binding site was necessary and sufficient for the chromatin events to occur, while transcription and basal promoter elements were dispensable. Here we show that addition of the hormone antagonists RU486 or RU43044 to the previously hormone-induced MMTV promoter results in cessation of transcription and loss of chromatin remodeling and nucleosome positioning. In vivo footprinting demonstrated agonist- and RU486-induced GR binding to its DNA response element (GRE), while the other antagonist, RU43044, did not promote GR-GRE interaction. These results demonstrate that induction and maintenance of nucleosome positioning is an active process that requires constant 'pressure' of agonist-GR-recruited chromatin-modifying factor(s) rather than GR-DNA binding itself.
authors.affiliation: Department of Cell and Molecular Biology, The Medical Nobel Institute, Box 285, Karolinska Institutet, SE-17177 Stockholm, Sweden.
authors.forename: S
authors.initials: S
authors.lastname: Belikov
authors.forename: B
authors.initials: B
authors.lastname: Gelius
authors.forename: O
authors.initials: O
authors.lastname: Wrange
mesh_terms: D000818:Animals; D002843:Chromatin / Q000235:genetics / Q000502:physiology; D005260:Female; D005938:Glucocorticoids / Q000494:pharmacology* / Q000502:physiology*; D006727:Hormone Antagonists / Q000494:pharmacology*; D006889:Hydroxycorticosteroids; D008324:Mammary Tumor Virus, Mouse / Q000235:genetics*; D051379:Mice; D015735:Mifepristone / Q000494:pharmacology*; D009707:Nucleosomes / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D009865:Oocytes / Q000166:cytology / Q000502:physiology*; D011401:Promoter Regions, Genetic; D014176:Protein Biosynthesis; D051381:Rats; D011965:Receptors, Glucocorticoid / Q000235:genetics / Q000502:physiology*; D011994:Recombinant Proteins / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects / Q000502:physiology*; D014982:Xenopus laevis
chemical_list: D002843:Chromatin; D005938:Glucocorticoids; D006727:Hormone Antagonists; D006889:Hydroxycorticosteroids; D009707:Nucleosomes; D011965:Receptors, Glucocorticoid; D011994:Recombinant Proteins; C085917:RU 43044; D015735:Mifepristone