Expression of genes encoding Th1 cell-activating cytokines and lymphoid homing chemokines by chlamydia-pulsed dendritic cells correlates with protective immunizing efficacy.
We studied the expression of cytokines, chemokines, and chemokine receptors by the RNase protection assay in chlamydia-pulsed dendritic cells to better understand their potent anti-chlamydial immunizing properties. We found that chlamydia-pulsed dendritic cells express a complex profile of inflammatory and immunomodulatory molecules. These include CCR-7, interleukin-12, and interferon-induced protein 10, molecules that might influence the homing of pulsed dendritic cells to the site of chlamydial infection and the induction of a local protective CD4(+) Th1 cellular immunity.
Shaw
J H
JH
Laboratory of Intercellular Parasites, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rocky Mountain Laboratory, Hamilton, Montana 59840, USA.
Grund
V R
VR
Durling
L
L
Caldwell
H D
HD
D000818:Animals; D002465:Cell Movement; D002478:Cells, Cultured; D018925:Chemokines / Q000235:genetics*; D002690:Chlamydia Infections / Q000517:prevention & control*; D002692:Chlamydia trachomatis / Q000276:immunology*; D016207:Cytokines / Q000235:genetics*; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology* / Q000382:microbiology; D005260:Female; D015870:Gene Expression; D006367:HeLa Cells; D006801:Humans; D007114:Immunization; D007275:Injections, Intravenous; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D019707:Receptors, Chemokine / Q000235:genetics*; D013601:T-Lymphocytes / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology*
D018925:Chemokines; D016207:Cytokines; D019707:Receptors, Chemokine