title: Identification of differentially expressed genes in the denervated rat hippocampus by cDNA arrays.
abstract: To elucidate the molecular mechanism underlying the physiological responses to injury in the central nervous system, gene expression profiles in rodent hippocampus following perforant path transection were investigated using cDNA array hybridization. Of the 8000 arrayed clones, 47 exhibited differential expression by >3-fold difference in the denervated hippocampus from control, with 15 up-regulated and 22 down-regulated. They can be functionally assigned into several classes, among which the most prominent are those coding proteins involved in macromolecules synthesis and processing. Northern blot analysis verified the validation of the aforementioned array data. These results throw some new light on the physiological responses of the hippocampus to entorhinal deafferentation at molecular level.
authors.affiliation: Laboratory of Neurobiology, Institute of Physiology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue-Yang Road, 200031, P.R., Shanghai, China.
authors.forename: G
authors.initials: G
authors.lastname: Ying
authors.forename: C
authors.initials: C
authors.lastname: Huang
authors.forename: N
authors.initials: N
authors.lastname: Jing
authors.forename: C
authors.initials: C
authors.lastname: Zhou
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D001930:Brain Injuries / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology; D003714:Denervation / Q000009:adverse effects; D015536:Down-Regulation / Q000235:genetics; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology*; D006624:Hippocampus / Q000293:injuries / Q000378:metabolism* / Q000503:physiopathology; D009416:Nerve Regeneration / Q000235:genetics*; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D009473:Neuronal Plasticity / Q000235:genetics*; D009474:Neurons / Q000378:metabolism* / Q000473:pathology; D020411:Oligonucleotide Array Sequence Analysis; D019580:Perforant Pathway / Q000293:injuries / Q000378:metabolism / Q000503:physiopathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D015854:Up-Regulation / Q000235:genetics
chemical_list: D009419:Nerve Tissue Proteins; D000110:Acetylcholinesterase