Methods for the spiral Salmonella mutagenicity assay including specialized applications.
An automated approach to bacterial mutagenicity testing - the spiral Salmonella assay - was developed to simplify testing and to reduce the labor and materials required to generate dose-responsive mutagenicity information. This document provides the reader with an overview of the spiral assay and a discussion of its application for examining the mutagenic potential of pure compounds, complex environmental mixtures, and interactive effects. Guidelines for performing a routine spiral assay are presented, and alternative test methods intended to overcome a variety of technical difficulties (such as restricted sample availability, sample viscosity or volatility, etc.) are recommended. Methods for the computerized analysis of data and the interpretation of results are discussed.
Claxton
L D
LD
Environmental Carcinogenesis Division, US Environmental Protection Agency, Research Triangle Park, NC 27711, USA. claxton.larry@epa.gov
Houk
V S
VS
Warren
S
S
D000818:Animals; D001331:Automation; D001431:Bacteriological Techniques / Q000295:instrumentation; D001711:Biotransformation; D003470:Culture Media; D004347:Drug Interactions; D004867:Equipment Design; D005798:Genes, Bacterial / Q000187:drug effects; D005838:Genotype; D007202:Indicators and Reagents; D008862:Microsomes, Liver / Q000201:enzymology; D009152:Mutagenicity Tests / Q000295:instrumentation / Q000379:methods*; D009153:Mutagens / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology; D009994:Osmolar Concentration; D051381:Rats; D012449:Safety; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development; D012984:Software
D003470:Culture Media; D007202:Indicators and Reagents; D009153:Mutagens