title: Candida albicans cell wall lytic enzyme produced by Streptomyces thermodiastaticus.
abstract: The production of lytic enzyme by Streptomyces thermodiastaticus was found to be affected by some growth conditions and nutritional factors. The highest enzyme production was obtained after 18 h of incubation at pH 5.5 and at 50 degrees C. The carbon source influenced the lytic enzyme production. A higher enzyme yield was obtained when Candida albicans cell wall (1 g/100 ml) was used as the sole carbon source. NaNO3 at 0.1 g/100 ml was the best nitrogen source for enzyme production. From all phosphorous sources, microelements, and growth factors tested, KH2PO4 (1 g/l), ZnSO4 (1 mg/I) and Tween 80 (0.1%), respectively, were found to favour the highest production of lytic enzymes by S. thermodiastaticus. The lytic enzymes mainly produced chitinolytic and proteolytic activities.
authors.affiliation: Department of Botany, Faculty of Science, Mansoura University, Egypt.
authors.forename: F A
authors.initials: FA
authors.lastname: Mansour
authors.forename: A H
authors.initials: AH
authors.lastname: Mohamedin
mesh_terms: D018533:Biomass; D002176:Candida albicans / Q000378:metabolism*; D002473:Cell Wall / Q000201:enzymology; D002688:Chitinases / Q000096:biosynthesis / Q000378:metabolism; D010450:Endopeptidases / Q000096:biosynthesis / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D013302:Streptomyces / Q000201:enzymology* / Q000378:metabolism; D013696:Temperature; D013997:Time Factors
chemical_list: D002688:Chitinases; D010450:Endopeptidases