A simple method for measuring EEG in freely moving guinea pigs.
This paper describes a method of measuring EEG in guinea pigs by means of temporarily attached superficial electrodes with radiotelemetry equipment. This represents an alternative approach to more conventional methods of monitoring EEG in animals, which necessitate surgical implantation of electrodes. The novel approach of using superficial electrodes was compared with the cortical screw electrode technique, in a nerve agent-induced seizure model. In both cases, radiotelemetry techniques were used for data capture to facilitate monitoring of nerve agent-induced seizures in freely moving non-tethered guinea pigs. The advantages and disadvantages of the technique are discussed, for example the saving in resources and the benefits to animal welfare.
Mumford
H
H
Biomedical Sciences Department, DERA, CBD Porton Down, Wiltshire SP4 0JQ, Salisbury, UK. hmumford@dera.gov.uk
Wetherell
J R
JR
D000818:Animals; D000927:Anticonvulsants / Q000494:pharmacology; D002540:Cerebral Cortex / Q000187:drug effects / Q000502:physiology*; D002619:Chemical Warfare Agents / Q000494:pharmacology; D003292:Convulsants / Q000494:pharmacology; D004566:Electrodes / Q000592:standards*; D004569:Electroencephalography / Q000187:drug effects / Q000295:instrumentation* / Q000379:methods*; D006168:Guinea Pigs; D008297:Male; D009068:Movement / Q000187:drug effects / Q000502:physiology; D012640:Seizures / Q000139:chemically induced / Q000503:physiopathology; D013686:Telemetry / Q000295:instrumentation* / Q000379:methods*
D000927:Anticonvulsants; D002619:Chemical Warfare Agents; D003292:Convulsants