title: High-performance liquid chromatographic method for the determination of di(2-ethylhexyl) phthalate in total parenteral nutrition and in plasma.
abstract: A simple, rapid and sensitive reversed-phase high-performance liquid chromatographic method with UV detection was developed for the quantification of di(2-ethylhexyl) phthalate (DEHP) in parenteral nutrition admixtures containing fat emulsion and in plasma samples of children daily treated by total parenteral nutrition. The analyte and the internal standard, di-n-heptyl phthalate, were extracted twice using hexane and the organic layer separated and dried under nitrogen. The residues were reconstituted with acetonitrile and 20 microl was injected into a Waters Spherisorb C18 column, the UV detector was set at 202 nm. The mobile phase was acetonitrile-aqueous buffer (triethylamine 0.08% adjusted to pH 2.8 with 1 M phosphoric acid) mixture (88:12, v/v) and it was pumped at 1 ml/min. Average recoveries were 97% or greater. This method was successfully used to investigate the amounts of DEHP which can leach from bags and tubing into fat emulsion and which could contaminate children under long-term parenteral nutrition. On the other hand, the circulating DEHP concentrations were estimated in four children under regular long-term parenteral nutrition.
authors.affiliation: Laboratoire de Pharmacologie, Pharmacocinétique et Pharmacie Clinique, Faculté des Sciences Pharmaceutiques et Biologiques, Lille, France.
authors.forename: K
authors.initials: K
authors.lastname: Kambia
authors.forename: T
authors.initials: T
authors.lastname: Dine
authors.forename: B
authors.initials: B
authors.lastname: Gressier
authors.forename: A F
authors.initials: AF
authors.lastname: Germe
authors.forename: M
authors.initials: M
authors.lastname: Luyckx
authors.forename: C
authors.initials: C
authors.lastname: Brunet
authors.forename: L
authors.initials: L
authors.lastname: Michaud
authors.forename: F
authors.initials: F
authors.lastname: Gottrand
mesh_terms: D002648:Child; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004051:Diethylhexyl Phthalate / Q000032:analysis / Q000097:blood*; D005217:Fat Emulsions, Intravenous / Q000032:analysis; D006801:Humans; D010289:Parenteral Nutrition, Total
chemical_list: D005217:Fat Emulsions, Intravenous; D004051:Diethylhexyl Phthalate