Synthesis, purification and biodistribution of (205)Bi-DOTMP, visualizing bone deposition patterns with autoradiography.
A HPLC system has been developed for carrier free and rapid delivery in a physiological buffer of the alpha-particle emitting bone-seeking radiopharmaceutical (212)Bi-DOTMP. (205)Bi-DOTMP was synthesized and HPLC purified to mimic and visualize the deposition pattern in bony tissues of (212)Bi-DOTMP by autoradiography. Inhomogeneous bone deposits were found with the highest concentration in the bone matrix, the endosteum and in the growth zones of young mice. Analysis of urine samples showed that (205)Bi-DOTMP was cleared as an intact complex.
Hassfjell
S
S
Department of Chemistry, Nuclear Chemistry Section, University of Oslo, N-0315, Blindern, Oslo, Norway. s.p.hassfjell@kjemi.uio.no
Ingebrigtsen
K
K
Bruland
Ø S
ØS
D000818:Animals; D001345:Autoradiography; D001729:Bismuth / Q000737:chemistry*; D001842:Bone and Bones / Q000000981:diagnostic imaging* / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D005260:Female; D051379:Mice; D008819:Mice, Nude; D009943:Organophosphorus Compounds / Q000138:chemical synthesis* / Q000302:isolation & purification / Q000493:pharmacokinetics*; D011868:Radioisotopes; D011877:Radionuclide Imaging; D019275:Radiopharmaceuticals / Q000138:chemical synthesis* / Q000302:isolation & purification / Q000493:pharmacokinetics*; D014018:Tissue Distribution
D009943:Organophosphorus Compounds; D011868:Radioisotopes; D019275:Radiopharmaceuticals; C082084:1,4,7,10-tetraazacyclododecane-1,4,7,10-tetrakis(methylenephosphonic acid); D001729:Bismuth