title: Histochemical study of acute and chronic intraperitoneal nicotine effects on several glycolytic and Krebs cycle dehydrogenase activities in the frontoparietal cortex and subcortical nuclei of the rat brain.
abstract: The effects of nicotine on the activity of different dehydrogenases in frontoparietal regions and subcortical nuclei of the rat brain have been studied using histochemical methods. Nicotine sulphate was intraperitoneally administered in acute (4 mg/kg/day x 3 days) or chronic (ALZET osmotic pump providing 2 mg/kg/day x 15 days) doses. The enzymes analyzed were glyceraldehyde-3-phosphate, lactate, malate and succinate dehydrogenases (gly3PDH, LDH, MDH, and SDH, respectively). The results demonstrate that chronic as well as acute administration of nicotine produced strong increases in all these enzymatic activities in the superior layers (I, II and III) of the frontoparietal cortex (cingulate, motor and somatosensory regions); but high increases were not seen in the deeper layers of the cortex or in the subcortical nuclei (substantia nigra, caudate-putamen, nucleus accumbens or nucleus basalis magnocellularis). These hyperactivities were produced in brain regions with normally low enzymatic activity (cortex), but not in those with great intensity (subcortical nuclei). The results are in rough agreement with previous reports on nicotine-induced increases in glucose utilization, gly3PDH genic expression and neuronal hyperactivity in the brain cortex; but significant discrepancies between the cortical enzymatic maps and those obtained both in these studies and others on nicotine(N)-receptor localization have been appreciated. The results support the hypothesis that nicotinic cholinergic drugs can have metabolic, long-lasting stimulant effects on cortical neurons at specific points (probably layer III pyramidal cells and structures with alpha7-N-receptors) of the cortical circuits that could be of great interest in improving altered cognitive functions that are present in Alzheimer disease, as well as in other less severe mental disturbances. Mitochondrial hyperfunction should also be evaluated as a possible side-effect (as an oxidative stress inductor) of these kinds of drugs.
authors.affiliation: Experimental Research Center, La Paz Hospital, INSALUD, Madrid, Spain.
authors.forename: L
authors.initials: L
authors.lastname: Turégano
authors.forename: R
authors.initials: R
authors.lastname: Martínez-Rodríguez
authors.forename: M I
authors.initials: MI
authors.lastname: Alvarez
authors.forename: R R
authors.initials: RR
authors.lastname: Gragera
authors.forename: A
authors.initials: A
authors.lastname: Gómez de Segura
authors.forename: E
authors.initials: E
authors.lastname: De Miguel
authors.forename: A
authors.initials: A
authors.lastname: Toledano
mesh_terms: D000818:Animals; D001479:Basal Ganglia / Q000187:drug effects / Q000201:enzymology; D020532:Basal Nucleus of Meynert / Q000187:drug effects / Q000201:enzymology; D002952:Citric Acid Cycle / Q000187:drug effects; D004789:Enzyme Activation / Q000187:drug effects; D005625:Frontal Lobe / Q000187:drug effects / Q000201:enzymology*; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism*; D006019:Glycolysis / Q000187:drug effects*; D006651:Histocytochemistry; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008291:Malate Dehydrogenase / Q000378:metabolism; D008297:Male; D008928:Mitochondria / Q000201:enzymology; D009538:Nicotine / Q000494:pharmacology*; D018722:Nicotinic Agonists / Q000494:pharmacology*; D010296:Parietal Lobe / Q000187:drug effects / Q000201:enzymology*; D051381:Rats; D017208:Rats, Wistar; D013378:Substantia Nigra / Q000187:drug effects / Q000201:enzymology; D013385:Succinate Dehydrogenase / Q000378:metabolism
chemical_list: D018722:Nicotinic Agonists; D009538:Nicotine; D007770:L-Lactate Dehydrogenase; D008291:Malate Dehydrogenase; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D013385:Succinate Dehydrogenase