Potential role of beta 1 integrin and collagen biosynthesis in estrogen-dependent reduction of apoptosis in tamoxifen-treated breast cancer cells.
It was found that 10 microM tamoxifen induced apoptosis and a significant (approximately 50%) depletion of beta 1 integrin levels in human breast cancer cells. Estradiol-treated MCF-7 cells exhibited exceptional viability and adherence, high levels of beta 1 integrin and increased (by 100%) collagen biosynthesis. Pretreatment of MCF-7 cells with 1 nM estradiol prevented tamoxifen-induced cell death, loss of cell adherence and decrease in beta 1 integrin level. Tamoxifen and estradiol had an opposite effect on the beta 1 integrin level and adherence in breast cancer cells, suggesting that the decrease in the beta 1 integrin level may be an early event during tamoxifen-induced apoptosis in breast cancer cells.
Dabrowska
M
M
Department of Hematological Diagnostics, Medical Academy of Bialystok, Bialystok, Poland. mdabrows@amb.ac.bialystok.pl
Palka
J
J
Wolczynski
S
S
Pietruczuk
M
M
Osada
J
J
D017209:Apoptosis / Q000187:drug effects*; D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology*; D002448:Cell Adhesion / Q000187:drug effects; D003094:Collagen / Q000096:biosynthesis*; D004958:Estradiol / Q000494:pharmacology*; D006801:Humans; D019012:Integrin beta1 / Q000032:analysis / Q000096:biosynthesis*; D009336:Necrosis; D013629:Tamoxifen / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D019012:Integrin beta1; D013629:Tamoxifen; D004958:Estradiol; D003094:Collagen