Computer-assisted evaluation of isoelectric focusing patterns in electrophoretic gels: identification of smoothhounds (Mustelus mustelus, Mustelus asterias) and comparison with lower value shark species.
In this work, the most commercially important Selachian species, smoothhound (Mustelus mustelus) and starry smoothhound (Mustelus asterias), have been identified by polyacrylamide isoelectric focusing (IEF-PAGE), along with several shark species of minor commercial value. In Italy, these two smoothhound species are commonly subjected to fraudulent substitution with lesser valued sharks. After the electrophoretic runs, the band patterns of the two Mustelus species were compared with those of dogfish (Scyliorhinus canicula), spurdog (Squalus acanthias), blue shark (Prionace glauca) and black-mouthed dogfish (Galeus melanostomus), both visually and with gel analysis software. The actual isoelectric points were then submitted to cluster analysis to differentiate the single species, despite the possible occurrence of electrophoretic variations or protein polymorphism. Every shark showed species-specific band patterns and could therefore be well differentiated, as confirmed by statistical analysis.
Renon
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P
Istituto di Ispezione degli Alimenti di Origine Animale, Facoltà di Medicina Veterinaria, Università degli Studi di Milano, Italy.
Colombo
M M
MM
Colombo
F
F
Malandra
R
R
Biondi
P A
PA
D000180:Acrylic Resins; D000818:Animals; D002816:Chordata, Nonvertebrate / Q000737:chemistry* / Q000145:classification; D001330:Electronic Data Processing; D007525:Isoelectric Focusing / Q000379:methods; D011506:Proteins / Q000032:analysis*; D012754:Sharks / Q000145:classification
D000180:Acrylic Resins; D011506:Proteins; C016680:polyacrylamide gels