Isotachophoretic determination of naproxen in the presence of its metabolite in human serum.
An isotachophoretic method with conductivity detection was developed to determine naproxen in the presence of its metabolite 6-O-desmethylnaproxen in human serum. The leading electrolyte contained 10 mM hydrochloric acid, beta-alanine, pH 4.0 and 0.1% methylhydroxypropylcellulose. The terminating electrolyte was 10 mM 2-(N-morpholino)ethanesulfonic acid-tris(hydroxymethyl)aminomethane, pH 6.9, containing 20% (v/v) of ethanol. Naproxen was determined in serum supernatant after simple deproteination of the sample with ethanol. The isotachophoretic results were compared with those obtained by synchronous fluorescence spectrometry.
Cakrt
M
M
Department of Analytical Chemistry, Faculty of Chemical Technology, Slovak University of Technology, Bratislava, Slovak Republic.
Hercegová
A
A
Lesko
J
J
Polonský
J
J
Sádecká
J
J
Skacáni
I
I
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000097:blood*; D002138:Calibration; D004586:Electrophoresis / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009288:Naproxen / Q000097:blood*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence
D000894:Anti-Inflammatory Agents, Non-Steroidal; D009288:Naproxen