Characterization of soluble protein extracts from keratinized tissues: identification of ubiquitin universally distributed in hair, nail, and stratum corneum.
Partial protein extracts were prepared from hair, nail, and stratum corneum in the absence of urea and interfacial surfactant. Tricine-sodium dodecyl sulfate polyacrylamide gel electrophoreses of these extracts showed low-molecular weight protein-rich patterns apparently different from those of whole protein extracts, which mainly consist of keratin bands. Several protein bands characterized each keratinized tissue or its derived species. In addition, we identified a major band of approximately 7 kDa as ubiquitin, a ubiquitously distributed protein that mediates non-lysosomal protein degradation, through direct amino acid sequence analysis of the electro-blotted protein band. The partial extraction is useful for investigation of soluble proteins retained in the keratinized tissues.
Inoue
T
T
Basic Research Laboratory, Kanebo Ltd., Odawara, Japan. tinoue@oda.cos.kanebo.co.jp
Kizawa
K
K
Ito
M
M
D000818:Animals; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D006168:Guinea Pigs; D006197:Hair / Q000737:chemistry*; D006801:Humans; D007633:Keratins / Q000737:chemistry; D051379:Mice; D009262:Nails / Q000737:chemistry*; D012333:RNA, Messenger / Q000032:analysis; D012867:Skin / Q000737:chemistry*; D014452:Ubiquitins / Q000737:chemistry*
D012333:RNA, Messenger; D014452:Ubiquitins; D007633:Keratins