Effects of combined treatment of rats with cadmium and ionizing radiation on nucleic acids in the kidneys, liver and haemopoietic organs.
The influence of Cd (1 mg/rat CdCl2 i.p.) and/or gamma radiation (6 Gy) on RNA and DNA content and/or concentration in the intact kidney and hypertrophic kidney (on the 44th hour after UN) and in other slowly and quickly proliferating organs was studied. The period between administration of Cd and Ir in the group with combined treatment was 30 min; between treatment (administration of Cd, Ir and combination of both treatments--Cd + Ir) and UN it was 1, 7, 14 and 21 days. The total extent of damage caused by the treatments in the investigated organs was following: intact kidney < liver < hypertrophic kidney and bone marrow < spleen < thymus. In the intact and hypertrophic kidney and liver, the administration of Cd caused more extensive changes in comparison with gamma irradiation, and the effects of combination of the treatments were similar to those of Cd alone. In the bone marrow, spleen and thymus, more profound changes were observed after Ir in comparison with Cd administration, and the effects of combined treatment were similar to the effects of Ir alone. The changes in the hypertrophic kidney after administration of Cd and/or Ir were more extensive than in the intact kidney, which suggests latent injury induction in the rat kidney by these noxa. The higher effectiveness of the treatments in the hypertrophic kidney than in the intact one was manifested mostly by the decrease in the RNA and DNA content, which was mainly due to inhibition of growth induced by UN and not by a real decrease in DNA and RNA contents caused by loss of damaged cells.
Slovinská
L
L
Department of Cellular and Molecular Biology, Faculty of Sciences, P. J. Safárik University, Kosice, Slovakia.
Kropácová
K
K
Kolesárová
M
M
Misúrová
E
E
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D002104:Cadmium / Q000494:pharmacology*; D004247:DNA / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D005720:Gamma Rays / Q000009:adverse effects; D006984:Hypertrophy; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D008297:Male; D009929:Organ Size; D012313:RNA / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D051381:Rats; D017208:Rats, Wistar; D013154:Spleen / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D013950:Thymus Gland / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects
D002104:Cadmium; D012313:RNA; D004247:DNA