Preparation of recombinant coat protein of Prunus necrotic ringspot virus.
The coat protein (CP) gene of Prunus necrotic ringspot virus (PNRSV) was cloned into pET 16b vector and expressed in Escherichia coli. CP-enriched fractions were prepared from whole cell lysate by differential centrifugation. The fraction sedimenting at 20,000 x g for 30 mins was used for preparation of a rabbit antiserum to CP. This antiserum had a titer of 1:2048 and reacted in a double-antibody sandwich ELISA (DAS-ELISA).
Petrzik
K
K
Department of Plant Virology, Institute of Plant Molecular Biology, Academy of Sciences of the Czech Republic, Ceské Budejovice.
Mráz
I
I
Kubelková
D
D
D001483:Base Sequence; D002213:Capsid / Q000235:genetics / Q000302:isolation & purification*; D003001:Cloning, Molecular; D005814:Genes, Viral; D019179:Ilarvirus / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification; D014197:Trees / Q000821:virology
D011994:Recombinant Proteins