A 1-kb Bcl-2-PCR fragment detection in a patient with follicular lymphoma and development of a new diagnostic PCR method.
The t(14;18) translocation is a useful marker to characterize follicular lymphoma and to monitor residual disease. The polymerase chain reaction (PCR) is a powerful technique to detect this translocation. Located on chromosome 18, within the Bcl-2 gene, breakpoints occur mainly in the 3; untranslated region, in the third exon of Bcl-2 (MBR region). In this study, the authors amplified MBR breakpoints by PCR and found an unexpectedly large fragment of 1 Kb that corresponds to a recently described new breakpoint in the Bcl-2 gene. With a new primer set, a further previously considered t(14;18)-unrelated tumor was in fact positive for this new breakpoint.
Hostein
I
I
Department of Molecular Oncology, Institut Bergonié, Bordeaux, France. ihostein@hotmail.com
Menard
A
A
Soubeyran
I
I
Eghbali
H
H
Debled
M
M
Gastaldello
B
B
Soubeyran
P
P
D001483:Base Sequence; D015139:Blotting, Southern; D019457:Chromosome Breakage; D002883:Chromosomes, Human, Pair 14; D002887:Chromosomes, Human, Pair 18; D017931:DNA Primers / Q000737:chemistry; D004273:DNA, Neoplasm / Q000032:analysis; D004587:Electrophoresis, Agar Gel; D019254:Genes, bcl-2 / Q000235:genetics*; D006801:Humans; D007124:Immunoenzyme Techniques; D008198:Lymph Nodes / Q000737:chemistry / Q000473:pathology; D008224:Lymphoma, Follicular / Q000737:chemistry / Q000235:genetics* / Q000473:pathology; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes / Q000737:chemistry; D016133:Polymerase Chain Reaction / Q000379:methods*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis; D014178:Translocation, Genetic / Q000235:genetics*
D017931:DNA Primers; D004273:DNA, Neoplasm; D015345:Oligonucleotide Probes; D019253:Proto-Oncogene Proteins c-bcl-2