title: Element concentrations and cataract: an experimental animal model.
abstract: The determination of inorganic ions in cataractous human lenses has been the subject of several investigations; nevertheless, few studies have been concerned with trace element contents in lenses, and data are sometimes contradictory. An animal experimental model of induced cataract is here proposed with the aim of evaluating the changes of Ca, Na, K, Cu and Zn concentrations. The cataract was produced by an Nd:YAG Laser treatment of the right eye of sexteen male rabbits. The determination of the elements was performed by atomic absorption spectrometry (both flame and flameless methods) after an acid digestion of samples. Compared with the results obtained in left lenses used as a control (Ca 14.4+/-5.7 mg/kg d.w.; Na 1.3+/-0.5 g/kg d.w.; K 9.9+/-1.1 g/kg d.w.; Cu 0.24+/-0.09 mg/kg d.w.; Zn 24.8+/-2.3 mg/kg d.w.), the mean concentration values of opaque lenses showed some significant changes for Ca, Na, and Cu (Ca 123.7+/-106.6 mg/kg d.w.; Na 4.5+/-4.3 g/kg d.w; Cu 0.43+/-0.21 mg/kg d.w.). Potassium showed a tendency to decrease, and zinc to increase. Positive correlations were found between calcium and sodium both in controls (r=0.73, p<0.001) and in treated lenses (r= 0.87, p<0.0001). An inverse correlation between Ca and K confirmed the tendency of potassium to decrease.
authors.affiliation: Istituto Superiore di Sanità, Applied Toxicology Department, Rome, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Ciaralli
authors.forename: R
authors.initials: R
authors.lastname: Giordano
authors.forename: S
authors.initials: S
authors.lastname: Costantini
authors.forename: A
authors.initials: A
authors.lastname: Sepe
authors.forename: F
authors.initials: F
authors.lastname: Cruciani
authors.forename: A
authors.initials: A
authors.lastname: Moramarco
authors.forename: B
authors.initials: B
authors.lastname: Antonelli
authors.forename: C
authors.initials: C
authors.lastname: Balacco-Gabrieli
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D002386:Cataract / Q000145:classification / Q000378:metabolism*; D003300:Copper / Q000378:metabolism; D004195:Disease Models, Animal; D007908:Lens, Crystalline / Q000378:metabolism*; D008297:Male; D011188:Potassium / Q000378:metabolism; D011817:Rabbits; D012964:Sodium / Q000378:metabolism; D014131:Trace Elements / Q000378:metabolism*; D015032:Zinc / Q000378:metabolism
chemical_list: D014131:Trace Elements; D003300:Copper; D012964:Sodium; D015032:Zinc; D011188:Potassium; D002118:Calcium