title: An improved heat pulse method to measure low and reverse rates of sap flow in woody plants.
abstract: The compensation heat pulse method (CHPM) is of limited value for measuring low rates of sap flow in woody plants. Recent application of the CHPM to woody roots has further illustrated some of the constraints of this technique. Here we present an improved heat pulse method, termed the heat ratio method (HRM), to measure low and reverse rates of sap flow in woody plants. The HRM has several important advantages over the CHPM, including improved measurement range and resolution, protocols to correct for physical and thermal errors in sensor deployment, and a simple linear function to describe wound effects. We describe the theory and methodological protocols of the HRM, provide wound correction coefficients, and validate the reliability and accuracy of the technique against gravimetric measurements of transpiration.
authors.affiliation: Department of Integrative Biology, University of California, Berkeley, CA 94720-3140, USA.
authors.forename: S S
authors.initials: SS
authors.lastname: Burgess
authors.forename: M A
authors.initials: MA
authors.lastname: Adams
authors.forename: N C
authors.initials: NC
authors.lastname: Turner
authors.forename: C R
authors.initials: CR
authors.lastname: Beverly
authors.forename: C K
authors.initials: CK
authors.lastname: Ong
authors.forename: A A
authors.initials: AA
authors.lastname: Khan
authors.forename: T M
authors.initials: TM
authors.lastname: Bleby
mesh_terms: D005052:Eucalyptus / Q000502:physiology; D006358:Hot Temperature; D018526:Plant Transpiration / Q000502:physiology; D013696:Temperature; D014197:Trees / Q000502:physiology*