Islet-brain1/JNK-interacting protein-1 is required for early embryogenesis in mice.
Islet-brain1/JNK-interacting protein-1 (IB1/JIP-1) is a scaffold protein that organizes the JNK, MKK7, and MLK1 to allow signaling specificity. Targeted disruption of the gene MAPK8IP1 encoding IB1/JIP-1 in mice led to embryonic death prior to blastocyst implantation. In culture, no IB1/JIP-1(-/-) embryos were identified indicating that accelerated cell death occurred during the first cell cycles. IB1/JIP-1 expression was detected in unfertilized oocytes, in spermatozoa, and in different stages of embryo development. Thus, despite the maternal and paternal transmission of the IB1/JIP-1 protein, early transcription of the MAPK8IP1 gene is required for the survival of the fertilized oocytes.
Thompson
N A
NA
Department of Internal Medicine and Institute of Cellular Biology and Morphology and the Reproductive Medicine Unit, CHUV-University Hospital, 1011 Lausanne, Switzerland.
Haefliger
J A
JA
Senn
A
A
Tawadros
T
T
Magara
F
F
Ledermann
B
B
Nicod
P
P
Waeber
G
G
D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D017209:Apoptosis; D001755:Blastocyst / Q000378:metabolism; D015153:Blotting, Western; D002352:Carrier Proteins / Q000502:physiology*; D016923:Cell Death; D002455:Cell Division; D002470:Cell Survival; D018076:DNA, Complementary / Q000378:metabolism; D004622:Embryo, Mammalian / Q000378:metabolism; D005260:Female; D006579:Heterozygote; D048688:MAP Kinase Kinase 7; D020930:MAP Kinase Kinase Kinases / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008856:Microscopy, Fluorescence; D048055:Mitogen-Activated Protein Kinase 8; D020929:Mitogen-Activated Protein Kinase Kinases / Q000378:metabolism; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D009687:Nuclear Proteins / Q000502:physiology*; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D013094:Spermatozoa / Q000378:metabolism; D013737:Testis / Q000378:metabolism; D015534:Trans-Activators / Q000502:physiology*; D014162:Transfection; D015053:Zygote / Q000378:metabolism
D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; D018076:DNA, Complementary; C490666:MAPK8IP1 protein, human; C490665:Mapk8ip protein, mouse; C110528:Mapk8ip1 protein, rat; D009687:Nuclear Proteins; D015534:Trans-Activators; D048055:Mitogen-Activated Protein Kinase 8; D020928:Mitogen-Activated Protein Kinases; D020930:MAP Kinase Kinase Kinases; C486650:Map3k9 protein, mouse; C486651:Map3k9 protein, rat; D048688:MAP Kinase Kinase 7; C482569:MAP2K7 protein, human; C482570:Map2k7 protein, mouse; D020929:Mitogen-Activated Protein Kinase Kinases