Direct resolution of (+/-)-ephedrine and atropine into their enantiomers by impregnated TLC.
Direct resolution of (+/-)-ephedrine and atropine into their enantiomers was achieved by normal-phase thin layer chromatography on silica gel plates impregnated with optically pure L-tartaric acid and L-histidine, respectively, as chiral selectors. The mobile phases enabling successful resolution were different combinations of acetonitrile-methanol-water. The spots were detected with iodine vapours and the detection limits were 2 and 6 microg, respectively, in terms of the racemate. The effects of concentration of the impregnating reagent, temperature and pH on resolution have been studied.
Bhushan
R
R
Department of Chemistry, University of Roorkee, Roorkee 247 667, India.
Martens
J
J
Arora
M
M
D000097:Acetonitriles; D001285:Atropine / Q000737:chemistry / Q000302:isolation & purification*; D002855:Chromatography, Thin Layer / Q000379:methods*; D004809:Ephedrine / Q000737:chemistry / Q000302:isolation & purification*; D006639:Histidine; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D000432:Methanol; D013237:Stereoisomerism; D013644:Tartrates; D013696:Temperature; D014867:Water
D000097:Acetonitriles; D007202:Indicators and Reagents; D013644:Tartrates; D014867:Water; D006639:Histidine; D001285:Atropine; D004809:Ephedrine; C029768:tartaric acid; D000432:Methanol; C032159:acetonitrile