title: Screening phage-displayed combinatorial peptide libraries.
abstract: Among the many techniques available to investigators interested in mapping protein-protein interactions is phage display. With a modest amount of effort, time, and cost, one can select peptide ligands to a wide array of targets from phage-display combinatorial peptide libraries. In this article, protocols and examples are provided to guide scientists who wish to identify peptide ligands to their favorite proteins.
authors.affiliation: Department of Pharmacology, University of Wisconsin-Madison, 1300 University Avenue, Madison, Wisconsin 53706-1532, USA. bkkay@facstaff.wisc.edu
authors.forename: B K
authors.initials: BK
authors.lastname: Kay
authors.forename: J
authors.initials: J
authors.lastname: Kasanov
authors.forename: M
authors.initials: M
authors.lastname: Yamabhai
mesh_terms: D020650:Combinatorial Chemistry Techniques; D004247:DNA / Q000138:chemical synthesis; D004797:Enzyme-Linked Immunosorbent Assay; D006863:Hydrogen-Ion Concentration; D019151:Peptide Library; D010957:Plasmids / Q000737:chemistry / Q000235:genetics; D011485:Protein Binding / Q000276:immunology*; D017422:Sequence Analysis, DNA
chemical_list: D019151:Peptide Library; D004247:DNA