A rapid and stable ITLC procedure for the determination of the radiochemical purity of 99Tcm-tetrofosmin.
99Tcm-tetrofosmin (Myoview, Amersham Healthcare) is widely used as a radiopharmaceutical for myocardial perfusion imaging. Control of its radiochemical purity after reconstitution is usually performed by means of ITLC-SG paper chromatography in a mobile phase of methylene chloride/acetone (65/35), as recommended by the manufacturer. The present study describes the application of tetrahydrofuran in phosphate buffer for the development of 99Tcm-tetrofosmin on ITLC-SG strips. The use of this mobile phase significantly improves the separation between labelled tetrofosmin and unbound pertechnetate. The time for development is about 1 min and the solvent is stable for at least 1 year. In addition, the volume spotted on the strip does not affect the migration of 99Tcm-tetrofosmin. The labelling efficiency of 99Tcm-tetrofosmin can successfully be monitored by means of this method as a daily routine procedure.
van Hemert
F J
FJ
Dept of Nuclear Medicine, Academic Medical Center, Amsterdam, The Netherlands. Formijn@Hotmail.com
Schimmel
K J
KJ
van Eck-Smit
B L
BL
D002854:Chromatography, Paper; D002855:Chromatography, Thin Layer; D004355:Drug Stability; D009943:Organophosphorus Compounds / Q000737:chemistry / Q000302:isolation & purification*; D015609:Organotechnetium Compounds / Q000737:chemistry / Q000302:isolation & purification*; D019275:Radiopharmaceuticals / Q000737:chemistry / Q000302:isolation & purification*; D013670:Sodium Pertechnetate Tc 99m / Q000302:isolation & purification; D012997:Solvents
D009943:Organophosphorus Compounds; D015609:Organotechnetium Compounds; D019275:Radiopharmaceuticals; D012997:Solvents; C078700:technetium tc-99m tetrofosmin; D013670:Sodium Pertechnetate Tc 99m