Light-induced biogenesis of chlorophyll-protein complexes in developing wheat thylakoids.
It is shown that the apoproteins of core complexes (CC) I and II, the alpha- and beta-subunits of CF1 ATP-synthase complexes, are present in seedlings grown under intermittent light (IML). The levels of light-harvesting complex (LHC) apoproteins in the 30- to 18-kD region increase rapidly upon exposure to continuous light (CL). The newly synthesized LHC apoproteins appear to be present predominantly in monomeric forms that later assemble into higher-order oligomeric forms. During the early stages of greening of wheat seedlings, polypeptides in the 20.5-19 and 17.5-15.5 kD regions (so-called "early light-induced proteins" (ELIP)) are observed, but they disappear fully after 6 h. As greening proceeds, the 727-nm band in low-temperature fluorescence spectra (77 K) gradually shifts to longer wavelength (740-742 nm), which clearly demonstrates the light-driven biogenesis of LHC I and its assembly with CC I.
Aliev
J A
JA
Institute of Botany, Academy of Sciences, Baku, 370073, Azerbaijan. dj_aliev@baku.ab.az
Guseinova
I M
IM
Suleimanov
S Y
SY
Zulfugarov
I S
IS
D004591:Electrophoresis, Polyacrylamide Gel; D008027:Light; D045342:Light-Harvesting Protein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism*; D010860:Pigments, Biological / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry / Q000378:metabolism; D010944:Plants / Q000737:chemistry / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D020524:Thylakoids / Q000737:chemistry / Q000378:metabolism*; D014908:Triticum / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism*
D045342:Light-Harvesting Protein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins; D010860:Pigments, Biological