An endoplasmic reticulum protein implicated in chaperoning peptides to major histocompatibility of class I is an aminopeptidase.
gp96, an abundant peptide-binding chaperone of the lumen of the endoplasmic reticulum and an acceptor of peptides transported into the endoplasmic reticulum through transporter associated with antigen processing, is shown to be an aminopeptidase. gp96 can trim an amino-terminal extended 19-mer precursor of the K(b)-binding VSV8 epitope for recognition by the cognate cytotoxic T lymphocyte clone. These observations support a role for gp96 in the amino-terminal trimming of extended peptides in the endoplasmic reticulum.
Ménoret
A
A
Center for Immunotherapy of Cancer and Infectious Diseases (MC1601), University of Connecticut School of Medicine, Farmington, Connecticut 06030, USA.
Li
Z
Z
Niswonger
M L
ML
Altmeyer
A
A
Srivastava
P K
PK
D000626:Aminopeptidases / Q000737:chemistry*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism; D000951:Antigens, Neoplasm / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000737:chemistry*; D000939:Epitopes; D005805:Genes, MHC Class I / Q000235:genetics*; D006863:Hydrogen-Ion Concentration; D015151:Immunoblotting; D051379:Mice; D018832:Molecular Chaperones / Q000378:metabolism*; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism; D017354:Point Mutation; D011485:Protein Binding; D011994:Recombinant Proteins / Q000378:metabolism; D016622:Silver Staining; D013601:T-Lymphocytes / Q000378:metabolism; D013696:Temperature; D014721:Vesicular stomatitis Indiana virus / Q000737:chemistry
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D000939:Epitopes; D018832:Molecular Chaperones; D010455:Peptides; D011994:Recombinant Proteins; C052646:sarcoma glycoprotein gp96 rejection antigens; D010450:Endopeptidases; D000626:Aminopeptidases