Determination of ketorolac in human plasma by reversed-phase high-performance liquid chromatography using solid-phase extraction and ultraviolet detection.
An improved high-performance liquid chromatographic method has been developed to measure human plasma concentrations of the analgesic nonsteroidal anti-inflammatory drug ketorolac for use in pharmacokinetic studies. Samples were prepared for analysis by solid-phase extraction using Bond-Elut PH columns, with nearly complete recovery of both ketorolac and the internal standard tolmetin. The two compounds were separated on a Radial-Pak C18 column using a mobile phase consisting of water-acetonitrile-1.0 mol/l dibutylamine phosphate (pH 2.5) (30:20:1) and detected at a UV wavelength of 313 nm. Using only 250 microl of plasma, the standard curve was linear from 0.05 to 10.0 microg/ml.
Wang
Z
Z
Department of Anesthesiology, Northwestern University Medical School, Chicago, IL 60611, USA.
Dsida
R M
RM
Avram
M J
MJ
D000293:Adolescent; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000097:blood* / Q000302:isolation & purification / Q000627:therapeutic use; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D020910:Ketorolac / Q000097:blood* / Q000302:isolation & purification; D020911:Ketorolac Tromethamine / Q000493:pharmacokinetics / Q000627:therapeutic use; D008297:Male
D000894:Anti-Inflammatory Agents, Non-Steroidal; D020911:Ketorolac Tromethamine; D020910:Ketorolac