title: Evidence for a novel polymorphism affecting both N-linked glycosylation and ligand binding of the IgG receptor IIIB (CD16).
abstract: Immunoglobulin G Fc receptor IIIb (FcgammaRIIIb) is constitutively expressed on neutrophils, and has three allelic forms: FcgammaRIIIb-NA1, FcgammaRIIIb-NA2, and FcgammaRIIIb-SH. We identified two Japanese subjects in whom an A to G substitution at nt 221 changes asparagine (N) to serine (S) at amino acid position 45 in the FcgammaRIIIb-NA2 gene. FcgammaRIIIb-NA2-specific monoclonal antibodies (GRM1 and PEN1) did not bind to mutant neutrophils, which lack an N-linked glycosylation site. Furthermore, IgG3-mediated neutrophil phagocytosis by mutant was slightly increased as compared to wild-type donors (Note).
authors.affiliation: Department of Periodontology, Faculty of Dentistry, Niigata University, Niigata, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Yamamoto
authors.forename: N
authors.initials: N
authors.lastname: Sugita
authors.forename: T
authors.initials: T
authors.lastname: Kobayashi
authors.forename: K
authors.initials: K
authors.lastname: Okuda
authors.forename: J G
authors.initials: JG
authors.lastname: Van De Winkel
authors.forename: H
authors.initials: H
authors.lastname: Yoshie
mesh_terms: D000595:Amino Acid Sequence; D019943:Amino Acid Substitution / Q000235:genetics; D001483:Base Sequence; D002236:Carbohydrate Conformation; D005260:Female; D006031:Glycosylation; D006579:Heterozygote; D006801:Humans; D008024:Ligands; D008297:Male; D008969:Molecular Sequence Data; D009504:Neutrophils / Q000276:immunology; D010587:Phagocytosis / Q000235:genetics; D011110:Polymorphism, Genetic / Q000276:immunology*; D017452:Receptors, IgG / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D008024:Ligands; D017452:Receptors, IgG