Candida albicans cell wall lytic enzyme produced by Streptomyces thermodiastaticus.
The production of lytic enzyme by Streptomyces thermodiastaticus was found to be affected by some growth conditions and nutritional factors. The highest enzyme production was obtained after 18 h of incubation at pH 5.5 and at 50 degrees C. The carbon source influenced the lytic enzyme production. A higher enzyme yield was obtained when Candida albicans cell wall (1 g/100 ml) was used as the sole carbon source. NaNO3 at 0.1 g/100 ml was the best nitrogen source for enzyme production. From all phosphorous sources, microelements, and growth factors tested, KH2PO4 (1 g/l), ZnSO4 (1 mg/I) and Tween 80 (0.1%), respectively, were found to favour the highest production of lytic enzymes by S. thermodiastaticus. The lytic enzymes mainly produced chitinolytic and proteolytic activities.
Mansour
F A
FA
Department of Botany, Faculty of Science, Mansoura University, Egypt.
Mohamedin
A H
AH
D018533:Biomass; D002176:Candida albicans / Q000378:metabolism*; D002473:Cell Wall / Q000201:enzymology; D002688:Chitinases / Q000096:biosynthesis / Q000378:metabolism; D010450:Endopeptidases / Q000096:biosynthesis / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D013302:Streptomyces / Q000201:enzymology* / Q000378:metabolism; D013696:Temperature; D013997:Time Factors
D002688:Chitinases; D010450:Endopeptidases