[The possibilities of GC/MS and HPLC in the analysis of sugars and acids in natural matrices].
A GC/MS procedure is described for the simultaneous quantitation of the minor and major constituents of several natural matrices, including fruits, vegetables, mushrooms, drug/industrial plants and honeys, as their trimethylsilyl derivatives, from one solution, by one injection. Selected minor components have been determined on the basis of their characteristic fragment ions, in the presence of extremely high excess of saccharides of various degree of polymerization. Selective fragmentation of these minor compounds in the ion trap detector provided possibilities for distinguishing them. In the case of honeys the method permitted the simultaneous quantitation of o-phosphoric, malic, shikimic, citric/isocitric, quinic, margaric, oleic and stearic acids, 5-hydroxymethylfurfurol and proline with the extremely high sugar contents of honeys (fructose, glucose, galacturonic acid, inositol, sucrose, trehalose, turanose, maltose, gentiobiose, isomaltose, raffinose, erlose, melezitose, maltotriose, panose, isomaltotriose) and allowed the fast evaluation of sugar and acid constituents of fifteen honeys from various floral and geological origin. Results revealed that (i) the minor components varied in the concentration range of 0.0001 to 0.43%, and, (ii) together with the saccharides of honeys made up the total of identified and determined constituents from 87.8% to 98.5%. Quantitative evaluation of the minor constituents was performed on the basis of their selective fragment ion values with an average reproducibility of 6.7% (RSD).
Perlné
M I
MI
ELTE, Szervetlen és Analitikai Kémiai Tanszék, H-1518, Budapest 112, Pf. 32. perlne@para.chem.elte.hu
Horváth
K
K
Katona
Z
Z
D001685:Biological Factors / Q000737:chemistry*; D002241:Carbohydrates / Q000032:analysis*; D002264:Carboxylic Acids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005638:Fruit / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006722:Honey / Q000032:analysis; D007221:Industry; D004364:Pharmaceutical Preparations / Q000032:analysis; D014675:Vegetables / Q000737:chemistry
D001685:Biological Factors; D002241:Carbohydrates; D002264:Carboxylic Acids; D004364:Pharmaceutical Preparations