title: Increase in serum S100A1-B and S100BB during cardiac surgery arises from extracerebral sources.
abstract: BACKGROUND
  Elevated levels of serum S100B after coronary artery bypass grafting may arise from extracerebral contamination. Serum S100B content was analyzed in several tissues, and the two dimers S100A1-B and S100BB were analyzed separately in blood.
  
  
  METHODS
  Serum, shed blood, marrow, fat, and muscle were studied in patients undergoing coronary artery bypass grafting with cardiopulmonary bypass using suction either to the cardiotomy reservoir (group 1, n = 10) or to a cell-saving device (group 2, n = 10), or operated on off-pump (group 3, n = 10).
  
  
  RESULTS
  Serum S100B was sixfold higher in group 1 than in groups 2 and 3, which were identical. The same ratio between S100A1-B and S100BB was found in all groups. When compared with serum, S100B was 10(2) to 10(4) times higher in marrow, fat, muscle tissue, and shed blood.
  
  
  CONCLUSIONS
  Separate analysis of S100A1-B and S100BB did not distinguish between S100B of cerebral and extracerebral origin. The concept that S100B only originates in astroglial and Schwann cells is wrong. Fat, muscle, and marrow in mediastinal blood contain high levels of S100B. Cardiopulmonary bypass caused no increase in S100B.
authors.affiliation: Department of Cardiothoracic Anaesthetics and Intensive Care, Karolinska Hospital, Stockholm, Sweden. russell.anderson@kirugi.ki.se
authors.forename: R E
authors.initials: RE
authors.lastname: Anderson
authors.forename: L O
authors.initials: LO
authors.lastname: Hansson
authors.forename: O
authors.initials: O
authors.lastname: Nilsson
authors.forename: J
authors.initials: J
authors.lastname: Liska
authors.forename: G
authors.initials: G
authors.lastname: Settergren
authors.forename: J
authors.initials: J
authors.lastname: Vaage
mesh_terms: D000328:Adult; D000368:Aged; D001921:Brain / Q000378:metabolism*; D002135:Calcium-Binding Proteins / Q000097:blood*; D003937:Diagnosis, Differential; D005260:Female; D006801:Humans; D007431:Intraoperative Complications / Q000097:blood* / Q000175:diagnosis; D008297:Male; D008875:Middle Aged; D009414:Nerve Growth Factors / Q000097:blood*; D011237:Predictive Value of Tests; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins; D020521:Stroke / Q000097:blood* / Q000175:diagnosis; D014018:Tissue Distribution
chemical_list: D002135:Calcium-Binding Proteins; D009414:Nerve Growth Factors; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins; C110294:S100A1 protein; C579160:S100B protein, human