title: Mass spectrometric studies of the path of carbon in photosynthesis: positional isotopic analysis of (13)C-labelled C(4) to C(7) sugar phosphates.
abstract: The (EIMS) electron ionization mass spectrometric fragmentation patterns of the methoxime- and ethoxime-trimethylsilyl (TMS) derivatives of C(4) to C(7) sugars involved as phosphates in the Calvin pathway of photosynthesis in plants were analysed by gas chromatography/EIMS using specifically labelled (13)C analogs. In general, most but not all of the major ions in the mass spectra arise from single carbon-carbon bond cleavages of the straight-chain derivatives. The results confirm that GC/MS of the alkoxime-TMS derivatives is a viable method for measuring (13)C incorporations at individual carbon atoms in each of the sugar phosphates during photosynthetic experiments with (13)CO(2).
authors.affiliation: Research School of Chemistry, Australian National University, Canberra, ACT 0200, Australia. macleod@rsc.anu.edu.au
authors.forename: J K
authors.initials: JK
authors.lastname: MacLeod
authors.forename: I L
authors.initials: IL
authors.lastname: Flanigan
authors.forename: J F
authors.initials: JF
authors.lastname: Williams
authors.forename: J G
authors.initials: JG
authors.lastname: Collins
mesh_terms: D002244:Carbon / Q000737:chemistry / Q000378:metabolism; D002247:Carbon Isotopes; D008401:Gas Chromatography-Mass Spectrometry; D015394:Molecular Structure; D010788:Photosynthesis; D021241:Spectrometry, Mass, Electrospray Ionization; D013403:Sugar Phosphates / Q000737:chemistry / Q000378:metabolism*
chemical_list: D002247:Carbon Isotopes; D013403:Sugar Phosphates; D002244:Carbon