Capillary isoelectric focusing with UV-induced fluorescence detection.
Low-molecular-mass fluorescent compounds excitable in the near UV region with suitable acidobasic and electrophoretic properties are suggested as isoelectric point (pI) markers for isoelectric focusing (IEF) with UV photometric and UV excited fluorometric detection. The experimental set-up of capillary IEF with UV excited fluorometric detection and properties of new UV-induced fluorescent pI markers are given. The pI values of 18 new pI markers determined independently of IEF methods range from 2.1 to 10.3. The examples of separation of new pI markers together with derivatized proteins by capillary IEF with photometric or fluorometric detection are presented.
Horká
M
M
Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, Brno.
Willimann
T
T
Blum
M
M
Nording
P
P
Friedl
Z
Z
Slais
K
K
D007525:Isoelectric Focusing / Q000379:methods*; D007526:Isoelectric Point; D011506:Proteins / Q000032:analysis / Q000302:isolation & purification; D013050:Spectrometry, Fluorescence / Q000379:methods*; D014466:Ultraviolet Rays
D011506:Proteins