Subtraction hybridization in microplates: an improved method to generate strain-specific PCR primers.
An improved subtraction hybridization technique was developed and evaluated. The hybridization is performed in a microplate with the subtractor-DNA immobilized in the plate while the probe-DNA is in solution. After hybridization the probe-specific DNA can easily be removed from the microwell and submitted to further analysis. This new technique has been successfully applied to generate several strain-specific PCR-primers for Lactococcus lactis subsp. lactis, Pediococcus spec., Saccharomyces spec. and Listeria monocytogenes.
Zwirglmaier
K
K
Lehrstuhl für Mikrobiologie, Technische Universität München-Weihenstephan, Freising, Germany.
Wassill
L
L
Ludwig
W
W
Schleifer
K H
KH
D017931:DNA Primers; D005516:Food Microbiology; D013294:Lactococcus lactis / Q000235:genetics / Q000302:isolation & purification; D008089:Listeria monocytogenes / Q000235:genetics / Q000302:isolation & purification; D009693:Nucleic Acid Hybridization / Q000379:methods*; D010376:Pediococcus / Q000235:genetics / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000302:isolation & purification; D013045:Species Specificity
D017931:DNA Primers