A metabolic model for acetate uptake under anaerobic conditions by glycogen accumulating organisms: Stoichiometry, kinetics, and the effect of pH.
A metabolic model for the stoichiometry of acetate uptake under anaerobic conditions by an enriched culture of glycogen accumulating organisms (GAOs) was developed and tested by experimental studies. Glycogen served as the source of both reducing power and energy to drive the process of acetate uptake. The amount of glycogen consumed and poly-beta-hydroxyvalerate (PHV) accumulated in the cells increased with increasing pH, indicating that the energy requirements for acetate uptake increased with pH. The composition of the accumulated poly-beta-hydroxyalkanoates (PHAs) was adequately predicted using the assumption that acetyl-CoA and propionyl-CoA condense randomly to produce PHA. In addition, the rate of acetate uptake was strongly affected by the pH. The rate decreased with increasing pH and this dependence could be described with a saturation type of expression. A comparison of the rate of acetate uptake at low pH with the rates observed in enriched cultures of phosphorus accumulating organisms (PAOs) indicated that GAOs are able to compete effectively with PAOs in nutrient removal systems under certain conditions.
Filipe
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CD
Department of Environmental Engineering and Science, L. G. Rich Environmental Research Laboratory, Clemson Research Park, Clemson University, Clemson, South Carolina 29634-0919, USA.
Daigger
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GT
Grady
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CP
D000085:Acetates / Q000378:metabolism*; D000105:Acetyl Coenzyme A / Q000378:metabolism; D000214:Acyl Coenzyme A / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000693:Anaerobiosis; D018533:Biomass; D019149:Bioreactors; D001709:Biotechnology / Q000379:methods; D002138:Calibration; D002849:Chromatography, Gas; D006003:Glycogen / Q000378:metabolism*; D006019:Glycolysis; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008954:Models, Biological; D008956:Models, Chemical; D009243:NAD / Q000378:metabolism; D011091:Polyesters / Q000378:metabolism; D011422:Propionates / Q000378:metabolism; D013386:Succinates / Q000378:metabolism; D014631:Valerates / Q000378:metabolism
D000085:Acetates; D000214:Acyl Coenzyme A; D011091:Polyesters; D011422:Propionates; D013386:Succinates; D014631:Valerates; D009243:NAD; C009061:propionyl-coenzyme A; D000105:Acetyl Coenzyme A; C073314:poly(3-hydroxyvalerate); D000255:Adenosine Triphosphate; D006003:Glycogen