title: Semen sample collection in medium enhances the implantation rate following ICSI in patients with severe oligoasthenoteratozoospermia.
abstract: Ejaculation in medium increases the proportion of antibody-free spermatozoa in semen samples containing anti-sperm antibodies and thereby enhances the fertilization rate in vitro. The aim of this study was to investigate whether this technique is also beneficial in semen samples with severe oligoasthenoteratozoospermia (OAT) where bacteria and detritus are often present. A prospective randomized controlled trial was carried out to study the results of sperm preparation and fertilization and pregnancy rates after intracytoplasmic sperm injection (ICSI) for OAT. Of the 114 couples (one cycle per couple) studied between 1998 and 2000, 55 men were randomized to have semen collection into sterile dry pots (group A) and the remaining 59 had samples collected into 20 ml HEPES buffered Ham's F-10 medium with 10% human serum albumin (group B). In group B the ejaculates were incubated for 30 min and mixed gently. The samples were then processed by mild centrifugation and washing followed by a mini-swim-up technique. The ejaculates in group A were prepared by the swim-up procedure only. The overall fertilization rate was 71.8% and was similar in groups A (fertilization rate = 66.7%) and B (fertilization rate = 64.3%). In group A, 10/55 clinical pregnancies were recorded (pregnancy rate 18%), with an implantation rate (IR) of 6.9% per embryo. In group B, 16 of 59 patients conceived leading to significantly higher implantation (9.9%, P < 0.001) and clinical pregnancy rates (27%, P < 0.001). It is postulated that the addition of medium before liquefaction could inhibit the binding of bacteria and detritus to the sperm surface and may diminish DNA damage caused by reactive oxygen species, leading to improved efficiency of fertilization. The results demonstrate that the addition of HEPES buffered Ham's F-10 medium to sample collection pots significantly improves the pregnancy rate after ICSI in patients with severe OAT.
authors.affiliation: Department of Obstetrics and Gynaecology, University of Würzburg, Josef-Schneider-Strasse 4, D-97080 Würzburg, Germany. u.zollner@mail.uni-wuerzburg.de [corrected]
authors.forename: U
authors.initials: U
authors.lastname: Zollner
authors.forename: K P
authors.initials: KP
authors.lastname: Zollner
authors.forename: J
authors.initials: J
authors.lastname: Dietl
authors.forename: T
authors.initials: T
authors.lastname: Steck
mesh_terms: D002021:Buffers; D010064:Embryo Implantation; D004624:Embryo Transfer; D005260:Female; D006531:HEPES; D006801:Humans; D007248:Infertility, Male / Q000628:therapy; D008297:Male; D009845:Oligospermia / Q000628:therapy*; D011247:Pregnancy; D011446:Prospective Studies; D012661:Semen / Q000502:physiology*; D013048:Specimen Handling / Q000379:methods*; D013076:Sperm Count; D020554:Sperm Injections, Intracytoplasmic; D013081:Sperm Motility; D013094:Spermatozoa / Q000002:abnormalities*; D016896:Treatment Outcome
chemical_list: D002021:Buffers; D006531:HEPES