Sensitive measurement of positron emitters eluted from HPLC.
For sensitive analysis of the radioactive-metabolite in human PET, a radio-HPLC system coupled to a newly designed positron detector was constructed. The detector had the advantages of low noise level (1.7 +/- 1.0 cpm) and high sensitivity (32 +/- 1%) due to coincidence counting and large BGO crystals. Furthermore, the detector was easy to move, since a pair of the BGO housings coupled to photomultipliers was effectively arranged in parallel and a HPLC cell with different volume could be inserted between the BGO housing. This radio-HPLC system was useful for analyzing samples with low radioactivity. It was applied to the measurement of [11C]FLB457 in plasma, having high affinity and high selectivity with dopamine D2 receptors. Extremely low radioactivity of [11C]FLB457 (2500 dpm) could be analyzed by using the radio-HPLC system. The performance of this detector was compared with those of commercially available systems that had been used as sensitive detectors for HPLC.
Takei
M
M
Division of Advanced Technology for Medical Imaging, National Institute of Radiological Sciences, Chiba, Japan.
Kida
T
T
Suzuki
K
K
D000818:Animals; D001729:Bismuth / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D003042:Cocaine / Q000031:analogs & derivatives*; D006801:Humans; D051379:Mice; D008815:Mice, Inbred Strains; D011759:Pyrrolidines / Q000097:blood; D011834:Radiation Monitoring / Q000295:instrumentation / Q000379:methods; D011954:Receptors, Dopamine / Q000378:metabolism; D012457:Salicylamides / Q000097:blood; D014055:Tomography, Emission-Computed / Q000295:instrumentation / Q000379:methods
D011759:Pyrrolidines; D011954:Receptors, Dopamine; D012457:Salicylamides; C065065:FLB 457; C021279:(1R-(exo,exo))-3-(4-fluorophenyl)-8-methyl-8- azabicyclo(3.2.1)octane-2-carboxylic acid, methyl ester; D003042:Cocaine; D001729:Bismuth