Lack of protective action of cysteine against the fetotoxic effect of monocrotaline.
Monocrotaline (MCT), a pyrrolizidine alkaloid present in Crotalaria species, has hepatotoxic, nephrotoxic, pneumotoxic and fetotoxic effects. However, the toxic effects of exposure to MCT in adult rats can be prevented by cysteine. Thus, the present study was conducted to evaluate the possible prevention by cysteine of the toxic effects of MCT on pregnant rats. Thirty-six pregnant rats were used. The females in the experimental groups were fed ration containing 0.02% MCT, 0.02% MCT + 1% cysteine, or 1% cysteine from day 6 to day 21 of pregnancy; the control group was fed only common ration for the same period of time. All rats were killed on day 21 of pregnancy and their blood was collected for determination of liver and kidney function. General toxicity to pregnant dams was assessed. Fetuses were removed by caesarian section and embryofetotoxic parameters were examined. Results showed impaired body weight gain in rats fed MCT, with or without cysteine supplementation. Plasma levels of AST, ALT, LDH, GGT, urea and creatinine were increased in MCT animals compared to controls. The pathology study revealed lesions only in dams from the MCT group. The weights of the placentas and fetuses of the MCT and MCT + cysteine groups were significantly lower than those of the control group. Thus, the present data suggests some protective action of 1% of cysteine in ration against the toxic effects of MCT on the dams but not on the litter.
Soto-Blanco
B
B
Research Center for Veterinary Toxicology (CEPTOX), Department of Pathology, School of Veterinary Medicine, University of São Paulo, Av. Orlando Marques de Paiva 87, 05508-900, São Paulo, Brazil.
Medeiros
R M
RM
Guerra
J L
JL
Górniak
S L
SL
D000410:Alanine Transaminase; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001835:Body Weight; D003404:Creatinine / Q000097:blood; D003545:Cysteine / Q000627:therapeutic use*; D004435:Eating; D005260:Female; D005313:Fetal Death / Q000517:prevention & control; D005333:Fetus / Q000187:drug effects*; D008297:Male; D016686:Monocrotaline / Q000633:toxicity*; D009929:Organ Size; D010936:Plant Extracts / Q000633:toxicity; D011041:Poisoning / Q000097:blood / Q000517:prevention & control; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar; D017211:Treatment Failure; D014508:Urea / Q000097:blood
D010936:Plant Extracts; D016686:Monocrotaline; D014508:Urea; D003404:Creatinine; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D003545:Cysteine