Autofluorescence microscopy for the detection of nematode eggs and protozoa, in particular Isospora suis, in swine faeces.
Parasites from swine faeces were examined for autofluorescence. Oocysts of Eimeria polita, E. scabra and Isospora suis, cysts of Balantidium coli and eggs of Oesophagostomum dentatum, Strongyloides ransomi and Trichuris suis (but not those of Ascaris suum) emitted light after excitation with UV light. I. suis oocyst counts in McMaster chambers utilising autofluorescence were compared to those from conventional bright field microscopy. Similarly, faecal smears containing I. suis were examined using the same techniques. Autofluorescence was superior to bright field microscopy in detecting oocysts after flotation and was highly significantly more sensitive when direct smears were examined.
Daugschies
A
A
Institute for Parasitology, School of Veterinary Medicine, Hannover, Germany. arwid.daugschies@tiho-hannover.de
Bialek
R
R
Joachim
A
A
Mundt
H C
HC
D000818:Animals; D005243:Feces / Q000469:parasitology*; D007549:Isospora / Q000254:growth & development / Q000302:isolation & purification*; D021865:Isosporiasis / Q000175:diagnosis / Q000662:veterinary*; D008018:Life Cycle Stages; D008856:Microscopy, Fluorescence / Q000379:methods / Q000662:veterinary*; D010270:Parasite Egg Count / Q000379:methods / Q000662:veterinary*; D012680:Sensitivity and Specificity; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis / Q000469:parasitology*