eIF4E association with 4E-BP decreases rapidly following fertilization in sea urchin.
The eukaryotic translation initiation factor (eIF) 4F facilitates the recruitment of ribosomes to the mRNA 5' end. The 4E-BPs are small proteins with hypophosphorylated forms that interact with the cap binding protein eIF4E, preventing its interaction with eIF4G, thereby preventing ribosome interaction with mRNA. In sea urchin, fertilization triggers a rapid rise in protein synthesis. Here, we demonstrate that a 4E-BP homologue exists and is associated with eIF4E in unfertilized eggs. We also show that 4E-BP/eIF4E association diminishes a few minutes following fertilization. This decrease is correlated with a decrease in the total amount of 4E-BP in combination with an increase in the phosphorylation of the protein. We propose that 4E-BP acts as a repressor of protein synthesis in unfertilized sea urchin eggs and that 4E-BP/eIF4E dissociation plays an important role in the rise in protein synthesis that occurs shortly following fertilization.
Cormier
P
P
Station Biologique de Roscoff, Université Pierre et Marie Curie (UFR 937), Roscoff Cedex, 29682, France. cormier@sb-roscoff.fr
Pyronnet
S
S
Morales
J
J
Mulner-Lorillon
O
O
Sonenberg
N
N
Bellé
R
R
D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D039561:Eukaryotic Initiation Factor-4E; D005260:Female; D005306:Fertilization / Q000502:physiology*; D008297:Male; D010063:Ovum / Q000378:metabolism; D010442:Peptide Chain Initiation, Translational; D010448:Peptide Initiation Factors / Q000378:metabolism*; D010766:Phosphorylation; D011233:Precipitin Tests; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism; D012617:Sea Urchins / Q000378:metabolism*
D002352:Carrier Proteins; D039561:Eukaryotic Initiation Factor-4E; D010448:Peptide Initiation Factors; D012333:RNA, Messenger