Quantitation of chondroitin-sulfates, disaccharides and hyaluronan in normal, early and advanced osteoarthritic sheep temporomandibular joints.
OBJECTIVE
To determine the relationship between synovial fluid, chondroitin sulfate disaccharide and hyaluronic acid to differing degrees of experimental temporomandibular joint (TMJ) osteoarthritis (OA).


METHODS
Twenty-four merino sheep were divided into three groups and had different TMJ surgical procedures to produce OA. Group I; control (six sheep), Group II; disc perforation (nine sheep) and Group III; disc perforation and articular damage (nine sheep). Synovial fluid was collected initially and at sacrifice at 3 months. Chondroitin 4-sulfate, chondroitin 6-sulfate and hyaluronic acid were measured and correlated to the OA histologic score.


RESULTS
The chondroitin-sulfate levels were significantly increased (Group I to Group II P< 0.001; Group I to Group III P< 0.001), the hyaluronic acid levels decreased (Group I to Group II P< 0.01; Group I to Group III P< 0.01) with the increasing OA score.


CONCLUSIONS
Chondroitin-sulfate and hyaluronic acid show a correlation with surgically created TMJ osteoarthritis in sheep model.
Ishimaru
J I
JI
Department of Oral and Maxillofacial Surgery, Gifu Prefectural Gifu Hospital, Japan. ishimaru@ktroad.ne.jp
Ogi
N
N
Mizuno
S
S
Goss
A N
AN
D000818:Animals; D002809:Chondroitin Sulfates / Q000378:metabolism*; D004187:Disaccharides / Q000378:metabolism*; D006820:Hyaluronic Acid / Q000378:metabolism*; D010003:Osteoarthritis / Q000378:metabolism*; D012756:Sheep; D013582:Synovial Fluid / Q000737:chemistry*; D013704:Temporomandibular Joint
D004187:Disaccharides; D006820:Hyaluronic Acid; D002809:Chondroitin Sulfates