Sugar uptake and utilisation in Streptomyces coelicolor: a PTS view to the genome.
Our research group is studying the phosphotransferase system (PTS) of Streptomyces coelicolor, which, in other bacteria, is centrally involved in carbon source uptake and regulation. We have surveyed the public available S. coelicolor genome sequence produced by the ongoing genome sequencing project for pts gene homologues (http://www.sanger.ac.uk/Projects/S_coelicolor/). Three genes encoding homologues of the general PTS components enzyme I (ptsI), HPr (ptsH), and enzyme IIA(Crr) (crr; IIA(Glc)-homologue) and six genes encoding homologues of sugar-specific PTS components were identified. The deduced primary sequences of the sugar-specific components shared significant similarities to PTS permeases of the mannitol/fructose family and of the glucose/sucrose family. A model is presented, in which possible functions of the novel described PTS homologues are discussed.
Parche
S
S
Lehrstuhl für Mikrobiologie, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany.
Nothaft
H
H
Kamionka
A
A
Titgemeyer
F
F
D000595:Amino Acid Sequence; D001419:Bacteria / Q000145:classification / Q000235:genetics; D001426:Bacterial Proteins; D001692:Biological Transport; D016384:Consensus Sequence; D029968:Escherichia coli Proteins; D019143:Evolution, Molecular; D005816:Genetic Complementation Test; D016680:Genome, Bacterial; D008969:Molecular Sequence Data; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D017852:Phosphotransferases (Nitrogenous Group Acceptor) / Q000235:genetics; D010802:Phylogeny; D010957:Plasmids; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D012984:Software; D013302:Streptomyces / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism*
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; C032242:crr protein, E coli; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; C030182:phosphocarrier protein HPr; D017852:Phosphotransferases (Nitrogenous Group Acceptor); C022360:phosphoenolpyruvate-protein phosphotransferase