Expression and induction of collagenases (MMP-8 and -13) in plasma cells associated with bone-destructive lesions.
Matrix metalloproteinases (MMPs) collectively degrade extracellular matrix and basement membrane proteins in chronic inflammation and bone-destructive lesions. This study examined the ability of immunoglobulin-producing plasma cells, typically present in sites of chronic inflammation, to express collagenases (MMP-8 and -13) in vivo and in vitro. Phorbol-12-myristate-13-acetate, interleukin-6, and tumour necrosis factor-alpha and heparin with the tumour promoter or cytokines potently enhanced (up to nine-fold) MMP-8 and -13 expression by the RPMI 8226 myeloma cell line, as evidenced by western blotting and semi-quantitative reverse transcriptase-polymerase chain reaction. Immunohistochemical analysis and in situ hybridization revealed that plasma cells expressed MMP-8 and -13 focally in periapical granulomas, odontogenic cysts, and malignant plasmacytomas. MMP-8 and MMP-13 from plasma cells can participate in bone organic matrix destruction at sites of chronic inflammation and neoplastic growth. Since MMP-13 was more frequently expressed than MMP-8 in plasma cells of strongly recurring keratocysts and malignant plasmacytomas, it is concluded that plasma cell MMP-13 has a particularly important role in benign and malignant bone-destructive lesions.
Wahlgren
J
J
Faculty of Medicine and Biomedicum, University of Helsinki, Finland. jaaharko@hammas.helsinki.fi
Maisi
P
P
Sorsa
T
T
Sutinen
M
M
Tervahartiala
T
T
Pirilä
E
E
Teronen
O
O
Hietanen
J
J
Tjäderhane
L
L
Salo
T
T
D001847:Bone Diseases / Q000201:enzymology*; D001859:Bone Neoplasms / Q000201:enzymology; D016009:Chi-Square Distribution; D017364:Collagenases / Q000032:analysis / Q000235:genetics; D006801:Humans; D015151:Immunoblotting; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D053509:Matrix Metalloproteinase 13; D020784:Matrix Metalloproteinase 8 / Q000032:analysis / Q000235:genetics; D020782:Matrix Metalloproteinases / Q000032:analysis*; D009101:Multiple Myeloma / Q000201:enzymology; D009807:Odontogenic Cysts / Q000201:enzymology; D010484:Periapical Granuloma / Q000201:enzymology; D010950:Plasma Cells / Q000201:enzymology*; D010954:Plasmacytoma / Q000201:enzymology; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured / Q000201:enzymology
D012333:RNA, Messenger; D017364:Collagenases; C507079:MMP13 protein, human; D053509:Matrix Metalloproteinase 13; D020782:Matrix Metalloproteinases; D020784:Matrix Metalloproteinase 8