title: RNA polymerase II and TBP occupy the repressed CYC1 promoter.
abstract: Saccharomyces cerevisiae CYC1 gene expression has been studied in great detail with regard to the response to oxygen availability and carbon source. In the absence of oxygen and the presence of glucose, the CYC1 gene is completely repressed. Chromatin structure is thought to play an important role in CYC1 gene regulation, as nucleosome depletion results in 94-fold derepression. In addition, the CYC1 core promoter has been used extensively in hybrid constructs to study activation by heterologous transcription factors. Therefore, we set out to map the chromatin structure of the CYC1 promoter and determine its role in CYC1 gene regulation. We report here that the repressed CYC1 promoter contains no positioned nucleosomes over the core promoter. However, we did find TFIID and RNA polymerase II bound in a complex on the repressed promoter. These results indicate that recruitment of TFIID and RNA polymerase II are not rate-limiting steps in CYC1 activation.
authors.affiliation: Department of Biochemistry and Molecular Biology, MRB Building, Room 231, Colorado State University, Fort Collins, CO 80523-1870, USA.
authors.forename: C
authors.initials: C
authors.lastname: Martens
authors.forename: B
authors.initials: B
authors.lastname: Krett
authors.forename: P J
authors.initials: PJ
authors.lastname: Laybourn
mesh_terms: D002843:Chromatin / Q000737:chemistry / Q000378:metabolism; D003432:Cross-Linking Reagents / Q000737:chemistry; D003574:Cytochrome c Group / Q000235:genetics* / Q000378:metabolism; D045304:Cytochromes c; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D008836:Micrococcal Nuclease / Q000378:metabolism; D009707:Nucleosomes / Q000378:metabolism; D011233:Precipitin Tests; D011401:Promoter Regions, Genetic; D012319:RNA Polymerase II / Q000235:genetics / Q000378:metabolism*; D012331:RNA, Fungal / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D016385:TATA Box; D035181:TATA-Box Binding Protein; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: C014059:CYC1 protein, S cerevisiae; D002843:Chromatin; D003432:Cross-Linking Reagents; D003574:Cytochrome c Group; D004268:DNA-Binding Proteins; D009707:Nucleosomes; D012331:RNA, Fungal; D029701:Saccharomyces cerevisiae Proteins; D035181:TATA-Box Binding Protein; D014157:Transcription Factors; D045304:Cytochromes c; D012319:RNA Polymerase II; D008836:Micrococcal Nuclease