Simultaneous determination of p-hydroxybenzaldehyde, p-hydroxybenzyl alcohol, 4-(beta-D-glucopyranosyloxy)-benzyl alcohol, and sugars in Gastrodia elata blume measured as their acetylated derivatives by GC-MS.
A method for the simultaneous separation and determination of the active constituents and three sugars in the roots of Gastrodia elata Blume (GE), which is used as a famous Chinese traditional herbal medicine, by gas chromatography-mass spectrometry is established. The samples are acetylated with pyridine-acetic anhydride. The contents of 4-hydroxybenzaldehyde, 4-hydroxybenzyl alcohol (HA), fructose, glucose, 4-(beta-D-glucopyranosyloxy)-benzyl alcohol (GA), and sucrose in GE are 0.004%, 0.03%, 1.36%, 1.12%, 1.97%, and 4.25%, respectively, and the detection limits are 1.5, 3.0, 11.0, 5.0, 33.0, and 35.0 pg, respectively. The contents of HA and GA in the urine and brain of a mouse are also determined. This method is simple, reliable, and quick for the simultaneous determination of the active constituents and sugars in GE.
Li
H X
HX
Chemistry Department of Tsinghua University, Beijing, China.
Ding
M Y
MY
Yu
J Y
JY
D000107:Acetylation; D000818:Animals; D001547:Benzaldehydes / Q000032:analysis* / Q000652:urine; D001592:Benzyl Alcohols / Q000032:analysis* / Q000652:urine; D001923:Brain Chemistry; D002241:Carbohydrates / Q000032:analysis* / Q000652:urine; D004365:Drugs, Chinese Herbal; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D005960:Glucosides / Q000032:analysis* / Q000652:urine; D019684:Magnoliopsida / Q000737:chemistry*; D051379:Mice; D012680:Sensitivity and Specificity
C429083:4-glucopyranosyloxybenzyl alcohol; D001547:Benzaldehydes; D001592:Benzyl Alcohols; D002241:Carbohydrates; D004365:Drugs, Chinese Herbal; D005960:Glucosides; C018966:4-hydroxybenzyl alcohol; C011483:4-hydroxybenzaldehyde