title: Epidemiologic Study of Pseudomonas aeruginosa in critical patients and reservoirs.
abstract: BACKGROUND
  Pseudomonas aeruginosa is a common cause of nosocomial infections, particularly in intensive care units (ICUs). The aim of this study was to characterize P. aeruginosa clinical isolates by comparing antimicrobial susceptibility patterns with the presence of plasmids and to establish the clonal relatedness by pulsed-field gel electrophoresis (PFGE) typing.
  
  
  METHODS
  The patients included those with isolation of P. aeruginosa hospitalized for more than 48 h in the ICU from April to May 1998. Environmental and staff cultures were obtained simultaneously. Minimal inhibitory concentrations, plasmid DNA profiles, and PFGE genomic patterns of enzyme restriction chromosomal DNA were compared.
  
  
  RESULTS
  Sixty P. aeruginosa isolates were obtained from 197 clinical specimens, 178 environmental samples, and 47 hand cultures of personnel. Antimicrobial resistance was as follows: tobramycin 100%; ticarcillin, cefotaxime, ceftriaxone, ceftazidime, and gentamicin 80%; cefepime 60%; amikacin, ticarcillin/clavulanate, imipenem, and meropenem 40%; piperacillin and norfloxacin 20%; carbenicillin 12%, and ciprofloxacin 0%. Plasmids were detected in 11 isolates (18%). PFGE typing showed that 23 isolates belonged to a common clone (pattern A), identified from five patients, two nurses, and 10 environmental samples. Ten isolates were grouped in four clusters and 27 isolates had unrelated genomic patterns. There was no relationship among DNA genomic patterns, plasmid profiles, and susceptibility patterns.
  
  
  CONCLUSIONS
  PFGE demonstrated the existence of a common clone in a critical care area. Reinforcement of infection control measures is needed to avoid horizontal transmission and severe infections.
authors.affiliation: Departamento de Infectología, Centro Médico del Occidente, Guadalajara, Jalisco, Mexico.
authors.forename: A L
authors.initials: AL
authors.lastname: Corona-Nakamura
authors.forename: M G
authors.initials: MG
authors.lastname: Miranda-Novales
authors.forename: B
authors.initials: B
authors.lastname: Leaños-Miranda
authors.forename: L
authors.initials: L
authors.lastname: Portillo-Gómez
authors.forename: A
authors.initials: A
authors.lastname: Hernández-Chávez
authors.forename: J
authors.initials: J
authors.lastname: Anthor-Rendón
authors.forename: S
authors.initials: S
authors.lastname: Aguilar-Benavides
mesh_terms: D016638:Critical Illness; D003428:Cross Infection / Q000150:complications / Q000453:epidemiology* / Q000382:microbiology; D004269:DNA, Bacterial; D004197:Disease Reservoirs; D004352:Drug Resistance, Microbial; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D010957:Plasmids; D011552:Pseudomonas Infections / Q000150:complications / Q000453:epidemiology* / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification
chemical_list: D004269:DNA, Bacterial