Adrenal gland SNAP-25 expression is altered in thyroid hormone receptor knock-out mice.
SNAP-25 is a protein in neurons and neuroendocrine cells, which is involved, together with syntaxin and VAMP, in neurotransmitter release and neurite outgrowth. Since the thyroid hormone receptors TR alpha and TR beta are essential for nervous system development, their possible role in regulating the expression of these vesicle trafficking proteins was examined by analysing SNAP-25 levels in TR alpha and TR beta knock-out mice. Immunoblotting and RT-PCR showed that SNAP-25 levels are increased in the adrenal gland, but not in cerebellum, in knock-out mice, while syntaxin-1 and VAMP-2 are unaffected in either tissue. Treatment of the pheochromocytoma-derived cell line PC12 with the thyroid hormone L-3,5,3'-triiodothyronine (T3) decreased SNAP-25 expression. Together, these data suggest that thyroid hormones exert a negative regulatory effect on SNAP-25 in adrenal medullary neuroendocrine cells.
Hepp
R
R
INSERM Unité 338, Biologie de la Communication Cellulaire, Strasbourg, France.
Grant
N J
NJ
Espliguero
G
G
Aunis
D
D
Sarlieve
L L
LL
Rodrigues-Pena
A
A
Langley
K
K
D000313:Adrenal Medulla / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism; D000818:Animals; D000954:Antigens, Surface / Q000378:metabolism; D002395:Catecholamines / Q000096:biosynthesis; D002531:Cerebellum / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D019439:Chromaffin Cells / Q000166:cytology / Q000378:metabolism*; D053379:Chromogranin A; D002864:Chromogranins / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects / Q000502:physiology; D008565:Membrane Proteins / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D018345:Mice, Knockout; D009419:Nerve Tissue Proteins / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D016716:PC12 Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D010446:Peptide Fragments / Q000378:metabolism; D021381:Protein Transport / Q000235:genetics*; D050683:R-SNARE Proteins; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011988:Receptors, Thyroid Hormone / Q000172:deficiency* / Q000235:genetics; D013570:Synaptic Membranes / Q000235:genetics* / Q000378:metabolism; D050825:Synaptosomal-Associated Protein 25; D050827:Syntaxin 1; D014284:Triiodothyronine / Q000378:metabolism / Q000494:pharmacology
D000954:Antigens, Surface; D002395:Catecholamines; D053379:Chromogranin A; D002864:Chromogranins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D010446:Peptide Fragments; D050683:R-SNARE Proteins; D012333:RNA, Messenger; D011988:Receptors, Thyroid Hormone; C494209:Snap25 protein, mouse; C494210:Snap25 protein, rat; C494214:Stx1a protein, mouse; C494213:Stx1a protein, rat; D050825:Synaptosomal-Associated Protein 25; D050827:Syntaxin 1; D014284:Triiodothyronine; C102106:vasostatin II