Assimilation of D-malate by Rhodobacter capsulatus E1F1.
Rhodobacter capsulatus grew by using either L- or D-malate as carbon sources under light/anaerobic conditions. The cellular yields were the same with D- or L-malate. Both L-malate dehydrogenase and L-malic enzyme activities were detected in cell-free extracts from cells grown in both isomers. By contrast, a racemase activity converting D-malate into L-malate was induced only when D-malate was present in the culture medium. This racemase activity was Mn2+-dependent and was measured by coupling it either to the malate dehydrogenase or to the fumarase activities. The racemase activity was partially purified by anion-exchange chromatography.
Martínez-Luque
M
M
Departamento de Bioquímica y Biología Molecular, Campus de Rabanales, Edificio C6, 1 Planta, 14071 Córdoba, Spain.
Castillo
F
F
Blasco
R
R
D002852:Chromatography, Ion Exchange; D003470:Culture Media; D004790:Enzyme Induction; D008291:Malate Dehydrogenase / Q000378:metabolism*; D008293:Malates / Q000378:metabolism*; D008345:Manganese / Q000494:pharmacology; D009243:NAD / Q000378:metabolism; D010084:Oxidation-Reduction; D019998:Racemases and Epimerases / Q000302:isolation & purification / Q000378:metabolism*; D016354:Rhodobacter capsulatus / Q000201:enzymology / Q000378:metabolism*; D013237:Stereoisomerism
D003470:Culture Media; D008293:Malates; D009243:NAD; D008345:Manganese; C030298:malic acid; D008291:Malate Dehydrogenase; C034544:D-malate dehydrogenase (decarboxylating); D019998:Racemases and Epimerases