title: Developmental expression, tissue distribution and hormonal regulation of fish (Sparus aurata) serum retinol-binding protein.
abstract: Retinol-binding protein (RBP) is the specific carrier of retinol in vertebrates and forms a 1:1 complex with transthyretin (TTR). A cDNA encoding serum RBP was cloned from liver and 7-day larvae of the marine fish Sparus aurata. The mature protein is 176 amino acids long and shows sequence identity of 77-78%, 56%, 63% and 62% with rainbow trout, Xenopus, chicken and human RBP, respectively. Northern blot analysis of hepatic RBP revealed two transcripts: a major one of approximately 1.4-1.5 kb and a minor of approximately 0.7 kb. Distribution of RBP mRNA in various tissues was studied by RT-PCR and showed high expression in liver and skin, and low expression in brain, kidney and gill filament (20-35% of the level in liver). RBP expression in intestine, pyloric caeca, muscle and pituitary was estimated to be approximately 7-14% of the level in liver. The ontogeny of RBP expression in S. aurata was examined in unfertilized eggs, embryos and larvae by using RT-PCR followed by hybridization with a specific probe. RBP transcript was found in all larval stages studied. Very low levels of RBP mRNA were detected in unfertilized eggs and in embryos 8 h after fertilization with a gradual increase at 12 h and 15-16 h post-fertilization. A single injection of estradiol-17beta to S. aurata immature, bisexual fish or to adult males reduced steady-state levels of hepatic RBP by 37 and 25%, respectively. The same treatment induced vitellogenin expression. The present data suggest that in fish, liver is the main site of RBP synthesis, but that RBP may have an important function in fish skin. RBP is expressed early in embryonic development and in fish its expression can be down regulated by estrogen.
authors.affiliation: National Institute of Oceanography, Israel Oceanographic and Limnological Research, Tel Shikmona, P.O. Box 8030, 31080, Haifa, Israel. bruria@ocean.org.il
authors.forename: B
authors.initials: B
authors.lastname: Funkenstein
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D004958:Estradiol / Q000494:pharmacology; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D008297:Male; D009928:Organ Specificity; D012313:RNA / Q000235:genetics / Q000378:metabolism; D012177:Retinol-Binding Proteins / Q000737:chemistry / Q000235:genetics*; D021541:Sea Bream / Q000196:embryology / Q000235:genetics* / Q000254:growth & development; D017386:Sequence Homology, Amino Acid
chemical_list: D012177:Retinol-Binding Proteins; D004958:Estradiol; D012313:RNA