Allopurinol suppresses para-nonylphenol and 1-methyl-4-phenylpyridinium ion (MPP(+))-induced hydroxyl radical generation in rat striatum.
We recently demonstrated that para-nonylphenol, an environmental estrogen-like chemical, enhances hydroxyl radical (*OH) generation in the rat striatum. In the present study we have examined whether para-nonylphenol enhanced 1-methyl-4-phenylpyridinium ion (MPP(+))-induced *OH generation in the rat striatum using a microdialysis technique. Para-nonylphenol significantly enhanced MPP(+)-induced *OH generation. Further, we studied the effect of allopurinol, a xanthine oxidase inhibitor, on para-nonylphenol and MPP(+)-induced *OH generation. Allopurinol significantly suppressed para-nonylphenol and MPP(+)-induced *OH generation. The results indicate that para-nonylphenol enhanced *OH generation based on superoxide anion production, and allopurinol may have preventive effect on para-nonylphenol and MPP(+)-induced *OH generation.
Obata
T
T
Department of Pharmacology, Oita Medical University, Hasama-machi, 879-5593, Oita, Japan. tobata@oita-med.ac.jp
Kubota
S
S
Yamanaka
Y
Y
D015655:1-Methyl-4-phenylpyridinium / Q000633:toxicity*; D000493:Allopurinol / Q000494:pharmacology*; D000818:Animals; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions / Q000502:physiology; D016166:Free Radical Scavengers / Q000494:pharmacology*; D015386:Hazardous Substances / Q000009:adverse effects; D017665:Hydroxyl Radical / Q000378:metabolism*; D008297:Male; D017551:Microdialysis; D017072:Neostriatum / Q000187:drug effects* / Q000378:metabolism / Q000503:physiopathology; D009410:Nerve Degeneration / Q000139:chemically induced / Q000378:metabolism / Q000503:physiopathology; D010300:Parkinson Disease / Q000378:metabolism / Q000503:physiopathology; D010302:Parkinson Disease, Secondary / Q000139:chemically induced*; D010636:Phenols / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar
D016166:Free Radical Scavengers; D015386:Hazardous Substances; D010636:Phenols; D017665:Hydroxyl Radical; D000493:Allopurinol; C041594:4-nonylphenol; D015655:1-Methyl-4-phenylpyridinium