Modelling production of extracellular polymeric substances in a Pseudomonas aeruginosa chemostat culture.
Formation of extracellular polymeric substances (EPSs) by mucoid Pseudomonas aeruginosa was investigated using literature data from chemostat cultures. The data were used to calibrate a product formation regime allowing substrate sufficient and endogenous EPS formation. Yield coefficients for both formation conditions were elucidated based on metabolic pathway analysis. Growth and non-growth related specific formation rates of 0.18 g CEPS/g Ccell/h and 0.03 1/h were estimated, respectively. The exogenous and endogenous EPS yield was found to be 0.77 g CEPS/g Cglu and 0.79 g CEPS/g Ccell, respectively. Being structurally equivalent to comprehensive maintenance models, this model allows for non-growth related product formation, showing the same quality of fit as previous models restricted to exogenous EPS precursors.
Kommedal
R
R
Telemark University College, Institute of Environmental Technology, Kjoelnes Ring 56, 3914 Porsgrunn, Norway. rok@imt.dtu.dk
Bakke
R
R
Dockery
J
J
Stoodley
P
P
D018441:Biofilms; D001704:Biopolymers / Q000096:biosynthesis*; D019149:Bioreactors; D005109:Extracellular Matrix / Q000378:metabolism*; D007700:Kinetics; D008954:Models, Biological; D011550:Pseudomonas aeruginosa / Q000254:growth & development / Q000378:metabolism*
D001704:Biopolymers