Reduced expression of the BRCA1 gene and increased chromosomal instability in MCF-7 cell line.
Using clonal cell cultures, a significant increase in chromosomal aberrations (aneuplolidy, dicentrics and chromatid breaks) were observed in MCF-7 cells compared with HeLa. BRCA1 expression was lower in MCF-7 cells than in HeLa cells. Since BRCA1 is known to play a role in the maintenance of chromosomal integrity, the increase in chromosomal aberrations in MCF-7 clones suggests that downregulation of BRCA1 expression could be one of the possible mechanisms for increased chromosomal instability in this cell line.
Kachhap
S K
SK
Cell Biology Division, Cancer Research Institute, Tata Memorial Centre, Parel, Mumbai 400012, India.
Vetale
S P
SP
Dange
P
P
Ghosh
S N
SN
D001943:Breast Neoplasms; D002869:Chromosome Aberrations / Q000503:physiopathology*; D025063:Chromosome Disorders; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D019398:Genes, BRCA1 / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D018895:Microsatellite Repeats; D012333:RNA, Messenger / Q000032:analysis
D012333:RNA, Messenger