The alternatively folded state of the antibody C(H)3 domain.
The C(H)3 domain of antibodies is characterized by two antiparallel beta-sheets forming a disulfide-linked sandwich-like structure. At acidic pH values and low ionic strength, C(H)3 becomes completely unfolded. The addition of salt transforms the acid-unfolded protein into an alternatively folded state exhibiting a characteristic secondary structure. The transition from native to alternatively folded C(H)3 is a fast reaction. Interestingly, this reaction involves the formation of a defined oligomer consisting of 12-14 subunits. Association is completely reversible and the native dimer is quantitatively reformed at neutral pH. This alternatively folded protein is remarkably stable against thermal and chemical denaturation and the unfolding transitions are highly cooperative. With a t(m) of 80 degrees C, the stability of the alternatively folded state is comparable to that of the native state of C(H)3. The defined oligomeric structure of C(H)3 at pH 2 seems to be a prerequisite for the cooperative unfolding transitions.
Thies
M J
MJ
Institut für Organische Chemie & Biochemie, Technische Universität München, Lichtenbergstr. 4, Garching, 85747, Germany.
Kammermeier
R
R
Richter
K
K
Buchner
J
J
D000143:Acids / Q000494:pharmacology; D000818:Animals; D000838:Anions / Q000494:pharmacology; D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000378:metabolism*; D002152:Calorimetry, Differential Scanning; D002850:Chromatography, Gel; D002942:Circular Dichroism; D006863:Hydrogen-Ion Concentration; D007127:Immunoglobulin Constant Regions / Q000737:chemistry* / Q000378:metabolism*; D007700:Kinetics; D008027:Light; D051379:Mice; D008970:Molecular Weight; D009994:Osmolar Concentration; D011489:Protein Denaturation / Q000187:drug effects; D017510:Protein Folding; D020836:Protein Structure, Quaternary / Q000187:drug effects; D017434:Protein Structure, Tertiary / Q000187:drug effects; D021122:Protein Subunits; D012492:Salts / Q000494:pharmacology; D012542:Scattering, Radiation; D012997:Solvents; D013696:Temperature; D013816:Thermodynamics; D014461:Ultracentrifugation
D000143:Acids; D000838:Anions; D000911:Antibodies, Monoclonal; D007127:Immunoglobulin Constant Regions; D021122:Protein Subunits; D012492:Salts; D012997:Solvents