Recombinant perciform GnRH-R activates different signaling pathways in fish and mammalian heterologous cell lines.
Perciforms have three forms of gonadotropin-releasing hormone (GnRH) in their brain. All three GnRHs are potent secretogogues for luteinizing hormone (LH) from the pituitary. The pivotal role of GnRH-R-GnRH interactions in reproductive homeostasis is well established; however, there is a paucity of information on how a GnRH-R responds to the three endogenous GnRH forms in a perciform species. In this study, a recombinant pituitary GnRH-R from striped bass (stb) was expressed in a mammalian cell line (COS-7) and a fish cell line (CHSE-214). Activation of the signaling pathways was monitored by reporter gene (luciferase) based assays, which were specific for cAMP-PKA or Ca 2+/calmodulin kinase (activated via c-fos promoter) signaling pathways. The stbGnRH-R expressed in two different cell lines triggered different downstream signaling in response to the treatments with chicken (c) GnRH II. Interestingly, when endogenous GnRHs were used in combinations, the luciferase activity was significantly attenuated in transfected CHSE-214 cells.
Alok
D
D
Center of Marine Biotechnology, University of Maryland Biotechnology Institute, 701 E. Pratt Street, 21202, Baltimore, MD, USA.
Kumar
R S
RS
Trant
J M
JM
Zohar
Y
Y
D000818:Animals; D001492:Bass; D002460:Cell Line; D002645:Chickens; D002522:Chlorocebus aethiops; D005399:Fishes; D017930:Genes, Reporter; D007987:Gonadotropin-Releasing Hormone / Q000494:pharmacology*; D011966:Receptors, LHRH / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012474:Salmon; D015398:Signal Transduction / Q000187:drug effects*; D013045:Species Specificity
D011966:Receptors, LHRH; D011994:Recombinant Proteins; D007987:Gonadotropin-Releasing Hormone