Development of a copro-antigen capture ELISA for detecting Ostertagia ostertagi infections in cattle.
The present study reports on the development of a copro-antigen capture ELISA for detecting Ostertagia ostertagi infections in cattle. The ELISA was based on polyclonal rabbit antibodies, which recognize O. ostertagi excretory/secretory antigens (ES). ES antigens are released by the metabolic active stages of the parasite in the abomasum, and passed in the faeces of the host. The detection limit of pure ES material was 30 ng ml(-1) in sample buffer and 125 ng ml(-1) in faecal extract. The test was evaluated using a follow up from six artificially infected calves. Elevated levels of Ostertagia coproantigens could be measured from 21 days after infection, indicating that only the presence of adult parasites can be detected. To evaluate the capacity of the assay to measure levels of infection, three groups of cattle were tested: 38 artificially infected calves, 17 naturally infected first grazing season calves and 16 naturally infected adult dairy cows. Optical densities were significantly correlated to the worm burdens of the animals and the ELISA had an overall sensitivity of 91% and a specificity of 45%. The test gave negative readings for faeces of animals carrying patent mono-infections with Cooperia oncophora.
Agneessens
J
J
Department of Parasitology, Faculty of Veterinary Medicine, Salisburylaan 133, B-9820, Merelbeke, Belgium. joost.agneessens@rug.ac.be
Claerebout
E
E
Vercruysse
J
J
D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis*; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000469:parasitology; D003612:Dairying; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary*; D005188:False Negative Reactions; D005243:Feces / Q000469:parasitology; D005260:Female; D007700:Kinetics; D010028:Ostertagia / Q000276:immunology*; D010029:Ostertagiasis / Q000175:diagnosis / Q000662:veterinary*; D010270:Parasite Egg Count / Q000662:veterinary; D012680:Sensitivity and Specificity
D000947:Antigens, Helminth