Study of the reaction of proteins with Beryllon II-AlIII by the Rayleigh light scattering technique and its application.
The determination of proteins with tetrasodium 2-(3,6-disulfo-8-hydroxynaphthylazo)-1,8-dihydroxynaphthalene-3,6-disulfonate (Beryllon II) by Rayleigh light scattering (RLS) was studied. The weak RLS of the Beryllon II-bovine serum albumin (BSA) complex can be greatly enhanced by the addition of Al3+ in the pH range 5.6-7.2; there was a maximum RLS platform at 400-420 nm. Based on the reaction between Beryllon II, Al3+ and proteins, a new method for the determination of proteins was developed. This method is very sensitive [0.20-41.42 micrograms ml-1 for BSA and 0.18-48.15 micrograms ml-1 for human serum albumen (HSA)], rapid (< 2 min), simple (one step) and tolerant towards most interfering substances. The effects of different surfactants were also examined. Four samples of protein in human serum were determined; the maximum relative error was no more than 5% and the recovery was 96-105%.
Dong
L
L
Department of Chemistry, Lanzhou University, Lanzhou, 730000, China.
Jia
R
R
Li
Q
Q
Chen
X
X
Hu
Z
Z
D000535:Aluminum; D001391:Azo Compounds / Q000737:chemistry*; D008027:Light; D009281:Naphthalenes / Q000737:chemistry*; D010777:Photochemistry / Q000379:methods*; D011506:Proteins / Q000032:analysis*; D012542:Scattering, Radiation
C431717:2-(3,6-disulfo-8-hydroxynaphthylazo)-1,8-dihydroxynaphthalene-3,6-disulfonate tetrasodium; D001391:Azo Compounds; D009281:Naphthalenes; D011506:Proteins; D000535:Aluminum