title: Group C rotavirus NSP4 induces diarrhea in neonatal mice.
abstract: Nonstructural glycoprotein NSP4 of group A rotavirus induces diarrhea in neonatal mice by functioning as an enterotoxin. Previously, our laboratory reported that the structural features of group A and group C rotavirus NSP4 proteins are well conserved despite a lack of sequence homology between group A and group C rotavirus NSP4 proteins [Horie Y, et al., Arch Virol (1997) 142: 1865-1872]. To test whether group C rotavirus NSP4 has an enterotoxigenic activity, we expressed in Escherichia coli the carboxy two-thirds (corresponding to amino acid residues 55-150) of the NSP4 protein derived from group C rotavirus strain Ehime 9301. This truncated NSP4 protein was able to induce diarrhea in 5-day-old CD-1 mice when administered intraperitoneally. Thus, group C rotavirus NSP4 acts as an enterotoxin like group A rotavirus NSP4.
authors.affiliation: Department of Microbiology, Akita University School of Medicine, Akita, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Sasaki
authors.forename: Y
authors.initials: Y
authors.lastname: Horie
authors.forename: T
authors.initials: T
authors.lastname: Nakagomi
authors.forename: M
authors.initials: M
authors.lastname: Oseto
authors.forename: O
authors.initials: O
authors.lastname: Nakagomi
mesh_terms: D000828:Animal Population Groups; D000818:Animals; D000831:Animals, Newborn; D003967:Diarrhea / Q000821:virology*; D004768:Enterotoxins / Q000235:genetics / Q000502:physiology; D004926:Escherichia coli / Q000235:genetics; D006023:Glycoproteins / Q000235:genetics / Q000502:physiology*; D051379:Mice; D012400:Rotavirus Infections / Q000821:virology*; D014118:Toxins, Biological; D014162:Transfection; D017361:Viral Nonstructural Proteins / Q000235:genetics / Q000502:physiology*
chemical_list: D004768:Enterotoxins; D006023:Glycoproteins; C081369:NS28 protein, rotavirus; D014118:Toxins, Biological; D017361:Viral Nonstructural Proteins