Parallel acceleration of evolutionary rates in symbiont genes underlying host nutrition.
The overproduction of essential amino acids by Buchnera aphidicola, the primary bacterial mutualist of aphids, is considered an adaptation for increased production of nutrients that are lacking in aphids' diet of plant sap. Given their shared role in host nutrition, amino acid biosynthetic genes of Buchnera are expected to experience parallel changes in selection that depend on host diet quality, growth rate, and population structure. This study evaluates the hypothesis of parallel selection across biosynthetic pathways by testing for correlated changes in evolutionary rates at biosynthetic genes of Buchnera. Previous studies show fast evolutionary rates at tryptophan biosynthetic genes among Buchnera associated with the aphid genus Uroleucon and suggest reduced purifying selection on symbiont nutritional functions in this aphid group. Here, we test for parallel rate acceleration at other amino acid biosynthetic genes of Buchnera-Uroleucon, including those for leucine (leuABC) and isoleucine/valine biosynthesis (ilvC). Ratios of nonsynonymous to synonymous substitutions (d(N)/d(S)) were estimated using codon-based maximum-likelihood methods that account for the extreme AT compositional bias of Buchnera sequences. A significant elevation in d(N)/d(S) at biosynthetic loci but not at two housekeeping genes sampled (dnaN and tuf) suggests reduced host-level selection on biosynthetic capabilities of Buchnera-Uroleucon. In addition, the discovery of trpEG pseudogenes in Buchnera-U. obscurum further supports reduced selection on amino acid biosynthesis.
Wernegreen
J J
JJ
Josephine Bay Paul Center for Comparative Molecular Biology and Evolution, The Marine Biological Laboratory, 7 MBL Street, Woods Hole, Massachusetts 02543, USA. jwernegreen@mbl.edu
Richardson
A O
AO
Moran
N A
NA
D000429:Alcohol Oxidoreductases / Q000235:genetics; D000596:Amino Acids / Q000096:biosynthesis*; D000818:Animals; D000878:Anthranilate Synthase / Q000235:genetics; D001042:Aphids / Q000235:genetics* / Q000378:metabolism / Q000382:microbiology; D020635:Buchnera / Q000235:genetics* / Q000378:metabolism; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D019143:Evolution, Molecular; D050540:Ketol-Acid Reductoisomerase; D008969:Molecular Sequence Data; D019884:Nitrogenous Group Transferases / Q000235:genetics; D010802:Phylogeny; D011544:Pseudogenes; D017422:Sequence Analysis, DNA; D013559:Symbiosis / Q000235:genetics
D000596:Amino Acids; D004269:DNA, Bacterial; D000429:Alcohol Oxidoreductases; D050540:Ketol-Acid Reductoisomerase; D019884:Nitrogenous Group Transferases; D000878:Anthranilate Synthase; C020655:anthranilate synthase, glutamine amidotransferase subunit