Purification of Piscirickettsia salmonis and associated phage particles.
Piscirickettsia salmonis was isolated from cell culture using differential centrifugation and purified on a 30% Percol gradient. The purity of the preparation was assessed by transmission electron microscopy and phage-like particles were found to be associated with some of the P. salmonis isolates examined. This is believed to be the first report of a phage associated with rickettsia from fish.
Yuksel
S A
SA
Institute of Aquaculture, University of Stirling, Scotland, UK.
Thompson
K D
KD
Ellis
A E
AE
Adams
A
A
D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient / Q000662:veterinary; D019084:Fluorescent Antibody Technique, Indirect / Q000662:veterinary; D020563:Gammaproteobacteria / Q000302:isolation & purification* / Q000472:pathogenicity; D008854:Microscopy, Electron / Q000662:veterinary; D012281:Rickettsia / Q000302:isolation & purification*; D012474:Salmon / Q000821:virology