title: Urinary type IV collagen excretion reflects renal morphological alterations and type IV collagen expression in patients with type 2 diabetes mellitus.
abstract: OBJECTIVE
  We tried to establish the significance of quantifying urinary type IV collagen (IV-C) excretion for the evaluation of renal involvement of type 2-diabetic patients.
  
  
  METHODS
  Twenty patients (13 males and 7 females; age range 31 to 69 years) with type 2 diabetes mellitus had undergone renal biopsy and relationship between the severity of morphological alteration, IV-C expression and urinary IV-C excretion were examined.
  
  
  RESULTS
  Urinary IV-C excretion significantly correlated with mesangial expansion score (p = 0.49, p < 0.05) and tubulointerstitial injury score (p = 0.56, p < 0.05). Furthermore, urinary IV-C excretion significantly correlated with both glomerular (r = 0.56, p < 0.01) and tubulointerstitial IV-C expression areas. Urinary protein excretion also correlated with mesangial expansion score and tubulointerstitial injury score. However, it did not correlate with the expression of IV-C in the kidney.
  
  
  CONCLUSIONS
  These results suggest that urinary IV-C excretion reflects the pathogenetic process of diabetic nephropathy, which urinary protein excretion alone cannot do sufficiently. It can be concluded that urinary IV-C excretion could be a more useful marker for the evaluation of renal involvement of type 2-diabetic patients.
authors.affiliation: Department of Internal Medicine, Sakura National Hospital, Chiba, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Okonogi
authors.forename: M
authors.initials: M
authors.lastname: Nishimura
authors.forename: Y
authors.initials: Y
authors.lastname: Utsunomiya
authors.forename: K
authors.initials: K
authors.lastname: Hamaguchi
authors.forename: H
authors.initials: H
authors.lastname: Tsuchida
authors.forename: Y
authors.initials: Y
authors.lastname: Miura
authors.forename: S
authors.initials: S
authors.lastname: Suzuki
authors.forename: T
authors.initials: T
authors.lastname: Kawamura
authors.forename: T
authors.initials: T
authors.lastname: Hosoya
authors.forename: K
authors.initials: K
authors.lastname: Yamada
mesh_terms: D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000652:urine; D003094:Collagen / Q000032:analysis / Q000652:urine*; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism / Q000473:pathology / Q000652:urine*; D003928:Diabetic Nephropathies / Q000175:diagnosis / Q000378:metabolism / Q000473:pathology; D005260:Female; D005920:Glomerular Mesangium / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D007668:Kidney / Q000737:chemistry / Q000473:pathology*; D007678:Kidney Glomerulus / Q000737:chemistry; D008297:Male; D008875:Middle Aged
chemical_list: D015415:Biomarkers; D003094:Collagen