Extraction and quantification of epibatidine in plasma.
Epibatidine was extracted from human and mouse plasma into a hexane-isopropanol mixture and back-extracted into a phosphate buffer, pH 2.5, then identified by HPLC isocratically using a CN column and quantified with ultraviolet detection at a fixed wavelength of 214 nm. The percent recovery of epibatidine from spiked plasma samples was 83.6% and the percent extraction was linear between 10 and 1,000 ng/ml. Desipramine was used as the internal standard. For spiked control samples containing 50 and 750 ng/ml, between-day precisions were 20.8 and 7.2% (RSD%), respectively; accuracy was 87.0 and 99.1%, respectively. The limit of detection was 2 ng/ml. Using this method, an intraperitoneal dose of 0.1 mg/kg of epibatidine produced mean levels of 7.3 and 37.1 ng/ml in pooled male and female plasma samples from C57BL/10 J mice, respectively. This is a simple and straightforward procedure by which plasma samples may be analyzed for epibatidine.
Javors
M A
MA
Department of Psychiatry, The University of Texas HSC, San Antonio 78229, USA. javors@uthscsa.edu
Sanchez
J J
JJ
King
T S
TS
Rohde
A R
AR
Wilson
S G
SG
Flores
C M
CM
D000818:Animals; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000097:blood* / Q000302:isolation & purification / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018722:Nicotinic Agonists / Q000097:blood* / Q000302:isolation & purification / Q000493:pharmacokinetics; D011725:Pyridines / Q000097:blood* / Q000302:isolation & purification / Q000493:pharmacokinetics; D015203:Reproducibility of Results; D012737:Sex Factors; D013045:Species Specificity
D019086:Bridged Bicyclo Compounds, Heterocyclic; D018722:Nicotinic Agonists; D011725:Pyridines; C082748:epibatidine