Hypertonicity-induced expression of aquaporin 3 in MDCK cells.
Aquaporins (AQPs) are water channel proteins that participate in water transport. In the principal cells of the kidney collecting duct, water reabsorption is mediated by the combined action of AQP2 in the apical membrane and both AQP3 and AQP4 in the basolateral membrane, and the expression of AQP2 and AQP3 is regulated by antidiuretic hormone and water restriction. The effect of hypertonicity on AQP3 expression in Madin-Darby canine kidney (MDCK) epithelial cells was investigated by exposing the cells to hypertonic medium containing raffinose or NaCl. Northern blot and immunoblot analyses revealed that the amounts of AQP3 mRNA and AQP3 protein, respectively, were markedly increased by exposure of cells to hypertonicity. These effects were maximal at 12 and 24 h, respectively. Immunofluorescence and immunoelectron microscopy also demonstrated that the abundance of AQP3 protein was increased in cells incubated in hypertonic medium and that the protein was localized at the basolateral plasma membrane. These results indicate that the expression of AQP3 is upregulated by hypertonicity.
Matsuzaki
T
T
Laboratory of Molecular and Cellular Morphology, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi, Gunma 371-8512, Japan.
Suzuki
T
T
Takata
K
K
D000818:Animals; D051400:Aquaporin 3; D020346:Aquaporins / Q000096:biosynthesis* / Q000235:genetics; D015152:Blotting, Northern; D002460:Cell Line; D004285:Dogs; D004848:Epithelium / Q000187:drug effects / Q000648:ultrastructure; D006982:Hypertonic Solutions; D015151:Immunoblotting; D007668:Kidney / Q000166:cytology / Q000378:metabolism; D007700:Kinetics; D008856:Microscopy, Fluorescence; D012333:RNA, Messenger / Q000378:metabolism; D011887:Raffinose / Q000494:pharmacology; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012965:Sodium Chloride / Q000494:pharmacology; D015854:Up-Regulation; D014867:Water / Q000378:metabolism
D020346:Aquaporins; D006982:Hypertonic Solutions; D012333:RNA, Messenger; D014867:Water; D051400:Aquaporin 3; D012965:Sodium Chloride; D011887:Raffinose