UV-detection capillary electrophoresis for benzo[a]pyrene and pyrene following a two-step concentration system using homogeneous liquid-liquid extraction and a sweeping method.
10(-9) mol l-1 levels of polycyclic aromatic hydrocarbons (PAHs) suspected for certain noxious materials can be determined and separated by UV-detection capillary electrophoresis following a two-step concentration system. When the conditions of the homogeneous liquid-liquid extraction were [THF]T = 5 vol%, [HCl]T = 0.66 mol l-1, and [PFOA]T = 2 x 10(-3) mol l-1 (i.e., the volume of sample solution; 50 ml-->sedimented phase; 30 microliters), the extraction percentages of benzo[a]pyrene (Bap) and pyrene (Py) at 10(-6) mol l-1 were 102 and 97.5%, respectively. Also, when the total concentration factor(volume ratio), which includes that of the sweeping method, was 8335-fold, the determination range of Bap was 6.4 x 10(-9)-8.0 x 10(-7) mol l-1 and for Py, 8.0 x 10(-9)-7.0 x 10(-7) mol l-1. The detection limits (3 sigma) of Bap and Py were 1.6 x 10(-9) and 4.8 x 10(-9) mol l-1, respectively.
Takagai
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Y
Department of Materials Science, Faculty of Engineering, Ibaraki University, 4-12-1 Nakanarusawa, Hitachi, Ibaraki 316-8511, Japan.
Igarashi
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S
D000393:Air Pollutants / Q000032:analysis*; D001564:Benzo(a)pyrene / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods; D005456:Fluorescent Dyes / Q000032:analysis; D011721:Pyrenes / Q000032:analysis; D014466:Ultraviolet Rays
D000393:Air Pollutants; D005456:Fluorescent Dyes; D011721:Pyrenes; D001564:Benzo(a)pyrene; C030984:pyrene