Quantification of L-ascorbic acid and total ascorbic acid in fruits and spinach by capillary zone electrophoresis.
A standard curve for the quantification of L-ascorbic acid (L-AA) by capillary zone electrophoresis (CZE) was established, and the quantification of ascorbic acid and total ascorbic acid in fruits (lemon, Sunkist, and pineapple) and spinach were performed using D-isoascorbic acid (D-IAA) as an internal standard. The minimum detection limits (MDLs) for L-AA and D-IAA were determined to be 1 and 2 microg/mL, respectively, at 265 nm. Dehydroascorbic acid (DHAA) in fruits and spinach was quantified in the presence of DL-homocysteine. The recoveries for L-AA in these juices were between 95 and 105%.
Liao
T
T
Graduate Institute of Food Science and Technology, National Taiwan University, Taipei.
Jiang
C M
CM
Wu
M C
MC
Hwang
J Y
JY
Chang
H M
HM
D001205:Ascorbic Acid / Q000032:analysis*; D002957:Citrus / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D005638:Fruit / Q000737:chemistry*; D018724:Spinacia oleracea / Q000737:chemistry*
C005056:isoascorbic acid; D001205:Ascorbic Acid