Stem cells and diabetes.
Diabetes mellitus is a metabolic disorder affecting 2-5% of the population. Transplantation of isolated islets of Langerhans from donor pancreata could be a cure for diabetes; however, such an approach is limited by the scarcity of the transplantation material and the long-term side effects of immunosuppressive therapy. These problems may be overcome by using a renewable source of cells, such as islet cells derived from stem cells. Stem cells are defined as clonogenic cells capable of both self-renewal and multilineage differentiation. This mean that these cells can be expanded in vivo or in vitro and differentiated to produce the desired cell type. There exist several sources of stem cells that have been demonstrated to give rise to pluripotent cell lines: 1) embryonic stem cells; 2) embryonic germ cells; 3) embryonic carcinoma cells; and 4) adult stem cells. By using in vitro differentiation and selection protocols, embryonic stem cells can be guided into specific cell lineages and selected by applying genetic selection when a marker gene is expressed. Recently, differentiation and cell selection protocols have been used to generate embryonic stem cell-derived insulin-secreting cells that normalise blood glucose when transplanted into diabetic animals. Some recent reports suggest that functional plasticity of adult stem cells may be greater than expected. The use of adult stem cells will circumvent the ethical dilemma surrounding embryonic stem cells and will allow autotransplantation. These investigations have increased the expectations that cell therapy could be one of the solutions to diabetes.
Berná
G
G
Institute of Bioengineering, University Miguel Hernández, Alicante, Spain
León-Quinto
T
T
Enseñat-Waser
R
R
Montanya
E
E
Martín
F
F
Soria
B
B
D002454:Cell Differentiation; D002460:Cell Line; D019070:Cell Lineage; D003920:Diabetes Mellitus / Q000628:therapy*; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D016381:Islets of Langerhans Transplantation; D013234:Stem Cells / Q000502:physiology*