Regulation of gene expression and cell growth in vivo by tetracycline using the hollow fiber assay.
The hollow fiber assay presents a potentially unique tool to study the effects of regulated gene expression in cell lines that do not form tumors in vivo. The hollow fibers allow small molecules to pass freely through while keeping the cells within the fibers and segregated from host cells. OSp16.1 cells, derived from the U24 clone of the U2-OS osteogenic sarcoma tumor line, express the p16INK4a tumor suppressor under the regulation of tetracycline (tet) (Mitra J et al. Mol Cell Bio 19:3916, 1999). The in vitro induction of p16 in the OSp16.1 cell line is regulated by tet. The hollow fiber assay was used to determine whether the regulation of the p16 gene could be achieved in vivo, since these cells did not grow in the xenograft model. There were no differences in the in vivo growth pattern of U24 cells loaded into the hollow fibers with and without tet: 807% and 839% net growth, respectively. OSp16.1 cells in fibers in mice receiving 3.33 mg/kg/day tet had a 644% net growth after 21 days. There was a 194% net growth without tet. Immunoblotting of extracts prepared from the hollow fibers confirmed that p16 was induced in the absence of tet. These data demonstrate this assay is a useful tool for studying the effects of regulated gene expression in vivo.
Krauthauser
C M
CM
DuPont Pharmaceuticals Co., Route 141 and Henry Clay Road, PO Box 80400, Wilmington, DE 19880-0400, USA.
Hall
L A
LA
Wexler
R S
RS
Slee
A M
AM
Mitra
J
J
Enders
G H
GH
Kerr
J S
JS
D000818:Animals; D002455:Cell Division; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000096:biosynthesis; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D008297:Male; D008567:Membranes, Artificial; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D011145:Polyvinyls; D012513:Sarcoma, Experimental / Q000378:metabolism*; D013752:Tetracycline / Q000494:pharmacology*; D014407:Tumor Cells, Cultured; D005465:Fluorocarbon Polymers
D019941:Cyclin-Dependent Kinase Inhibitor p16; D008567:Membranes, Artificial; D011145:Polyvinyls; D013752:Tetracycline; C024865:polyvinylidene fluoride; D005465:Fluorocarbon Polymers