title: Oleate oxidation and mitochondrial substrate selection in vascular smooth muscle.
abstract: The purpose of this study was to determine the contribution of long-chain fatty acids relative to other mitochondrial substrates in active vascular smooth muscle (VSM). Hog carotid arteries were isometrically contracted in physiological saline solution containing 0.71 mM U-(13)C-oleic acid (bound to albumin at a ratio of 6.8:1), 5 mM 1-(13)C-glucose and 1 mM acetate in the presence or absence of 5 mM carnitine for 6 h at 37 degrees C. Substrate oxidation was determined using (13)C-isotopomer analysis of glutamate. Although oxidation of oleic acid could not be measured at physiological concentrations [0.5 mM (1:1)], oleic acid oxidation was approximately 5% of the total substrates oxidized at the higher concentration examined. Although insignificant, carnitine increased oleic acid oxidation to approximately 8%, and resulted in a decrease in endogenous lipid oxidation, which was 2-12% of the total substrates oxidized. Oxidation of glucose and acetate did not significantly change due to the inclusion of oleic acid in the incubation solutions. Therefore, we conclude that exogenous long-chain fatty acids are minor contributors to substrate oxidation (approximately 5%) in VSM compared to other mitochondrial substrates, such as glucose and acetate, which account for approximately 80% of the substrates oxidized by VSM.
authors.affiliation: Department of Physiology, University of Missouri, Columbia, MO 65212, USA.
authors.forename: T J
authors.initials: TJ
authors.lastname: Allen
authors.forename: C D
authors.initials: CD
authors.lastname: Hardin
mesh_terms: D000085:Acetates / Q000378:metabolism; D000818:Animals; D002331:Carnitine / Q000494:pharmacology; D002339:Carotid Arteries / Q000187:drug effects; D002952:Citric Acid Cycle; D046508:Culture Techniques; D005947:Glucose / Q000378:metabolism; D018698:Glutamic Acid / Q000032:analysis; D019344:Lactic Acid / Q000096:biosynthesis; D009682:Magnetic Resonance Spectroscopy; D008928:Mitochondria / Q000378:metabolism*; D009131:Muscle, Smooth, Vascular / Q000378:metabolism*; D019301:Oleic Acid / Q000378:metabolism*; D010084:Oxidation-Reduction; D013379:Substrate Specificity; D013552:Swine
chemical_list: D000085:Acetates; D019301:Oleic Acid; D019344:Lactic Acid; D018698:Glutamic Acid; D005947:Glucose; D002331:Carnitine