title: Smooth muscle length-dependent PI(4,5)P2 synthesis and paxillin tyrosine phosphorylation.
abstract: We studied effects of increasing the length of porcine trachealis muscle on 5.5 microM carbachol (CCh)-evoked phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2] synthesis and other parameters of phosphatidylinositol (PI) turnover. PI(4,5)P2 resynthesis rates in muscle held at 1.0 optimal length (L(o)), measured over the first 6 min of CCh stimulation, were 140 +/- 12 and 227 +/- 14% of values found in muscle held at 0.5 L(o) and in free-floating muscle, respectively. Time-dependent changes in cellular masses of PI(4,5)P2, PI, and phosphatidic acid, and PI resynthesis rates, were also altered by the muscle length at which contraction occurred. In free-floating muscle, CCh did not evoke increases in tyrosine-phosphorylated paxillin (PTyr-paxillin), an index of beta1-integrin signaling; however, there were progressive increases in PTyr-paxillin in muscle held at 0.5 and 1.0 L(o) during contraction, which correlated with increases in PI(4,5)P2 synthesis rates. These data indicate that PI(4,5)P2 synthesis rates and other parameters of CCh-stimulated inositol phospholipid turnover are muscle length-dependent and provide evidence that supports the hypothesis that length-dependent beta1-integrin signals may exert control on CCh-activated PI(4,5)P2 synthesis.
authors.affiliation: Department of Physiology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA.
authors.forename: D
authors.initials: D
authors.lastname: Sul
authors.forename: C B
authors.initials: CB
authors.lastname: Baron
authors.forename: R
authors.initials: R
authors.lastname: Broome
authors.forename: R F
authors.initials: RF
authors.lastname: Coburn
mesh_terms: D000818:Animals; D002217:Carbachol / Q000494:pharmacology*; D018679:Cholinergic Agonists / Q000494:pharmacology*; D003598:Cytoskeletal Proteins / Q000378:metabolism*; D015151:Immunoblotting; D066298:In Vitro Techniques; D007294:Inositol / Q000378:metabolism; D019012:Integrin beta1 / Q000378:metabolism; D009130:Muscle, Smooth / Q000033:anatomy & histology / Q000187:drug effects / Q000378:metabolism*; D051419:Paxillin; D019269:Phosphatidylinositol 4,5-Diphosphate / Q000096:biosynthesis* / Q000378:metabolism; D010750:Phosphoproteins / Q000378:metabolism*; D010766:Phosphorylation; D015398:Signal Transduction; D013552:Swine; D014132:Trachea / Q000033:anatomy & histology
chemical_list: D018679:Cholinergic Agonists; D003598:Cytoskeletal Proteins; D019012:Integrin beta1; D051419:Paxillin; D019269:Phosphatidylinositol 4,5-Diphosphate; D010750:Phosphoproteins; D007294:Inositol; D002217:Carbachol