Protein kinase C isozyme-specific phosphorylation of profilin.
Profilin, a cytoskeletal protein, is emerging as an important link between signal transduction pathways and cytoskeletal dynamics. Profilin is phosphorylated on its C-terminal serine by protein kinase C (PKC). The protein kinase used for the in vitro phosphorylation studies reported earlier was a mixture of isozymes, and therefore, attempts were made to address the isozyme specificity on profilin phosphorylation under in vitro conditions. Profilin was subjected to phosphorylation by PKCalpha, PKCepsilon, and PKCzeta isozymes individually, and it was observed that profilin phosphorylation is cofactor-independent. PKCzeta phosphorylates profilin to a higher extent, but exhibits cofactor dependency with respect to phosphoinositides. The stoichiometry of phosphorylation was measured in the presence of these different isozymes, and a maximum stoichiometry of 0.8 (mole phosphate incorporated/mole profilin) was obtained in the presence of PKCzeta. Phosphorylation of profilin by PKCzeta was maximal in the presence of phosphatidylinositol4,5-bisphosphate (PI4,5-P2) when compared to the other phosphoinositides studied.
Vemuri
B
B
Department of Biochemistry, University College of Science, Osmania University, 500 007, A.P, Hyderabad, India.
Singh
S S
SS
D000818:Animals; D002417:Cattle; D002460:Cell Line; D003285:Contractile Proteins; D003599:Cytoskeleton / Q000378:metabolism*; D007313:Insecta; D007527:Isoenzymes / Q000737:chemistry / Q000378:metabolism; D008024:Ligands; D008081:Liposomes / Q000378:metabolism; D008840:Microfilament Proteins / Q000378:metabolism*; D019269:Phosphatidylinositol 4,5-Diphosphate / Q000378:metabolism; D010766:Phosphorylation; D051304:Profilins; D011493:Protein Kinase C / Q000737:chemistry* / Q000378:metabolism*; D051571:Protein Kinase C-alpha; D051744:Protein Kinase C-epsilon; D011994:Recombinant Proteins / Q000378:metabolism; D012694:Serine / Q000737:chemistry; D015398:Signal Transduction; D013154:Spleen / Q000737:chemistry; D013997:Time Factors; D000099221:Protein Kinase C zeta
D003285:Contractile Proteins; D007527:Isoenzymes; D008024:Ligands; D008081:Liposomes; D008840:Microfilament Proteins; D019269:Phosphatidylinositol 4,5-Diphosphate; D051304:Profilins; D011493:Protein Kinase C; D051571:Protein Kinase C-alpha; D051744:Protein Kinase C-epsilon; D011994:Recombinant Proteins; D012694:Serine; D000099221:Protein Kinase C zeta