title: Global gene expression during short-term ethanol stress in Saccharomyces cerevisiae.
abstract: DNA microarrays were used to investigate the expression profile of yeast genes in response to ethanol. Up to 3.1% of the genes encoded in the yeast genome were up-regulated by at least a factor of three after 30 min ethanol stress (7% v/v). Concomitantly, 3.2% of the genes were down-regulated by a factor of three. Of the genes up-regulated in response to ethanol 49.4% belong to the environmental stress response and 14.2% belong to the stress gene family. Our data show that in addition to the previously identified ethanol-induced genes, a very large number of genes involved in ionic homeostasis, heat protection, trehalose synthesis and antioxidant defence also respond to ethanol stress. It appears that a large number of the up-regulated genes are involved in energy metabolism. Thus, 'management' of the energy pool (especially ATP) seems to constitute an ethanol stress response and to involve different mechanisms.
authors.affiliation: UMR Sciences pour l'oenologie, Microbiologie et Technologie des fermentations, IPV, INRA, Place Viala, 34060 Montpellier Cedex 01, France.
authors.forename: H
authors.initials: H
authors.lastname: Alexandre
authors.forename: V
authors.initials: V
authors.lastname: Ansanay-Galeote
authors.forename: S
authors.initials: S
authors.lastname: Dequin
authors.forename: B
authors.initials: B
authors.lastname: Blondin
mesh_terms: D000431:Ethanol / Q000494:pharmacology*; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects*; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000032:analysis; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D000431:Ethanol