title: An efficient purification process for sweet potato beta-amylase by affinity precipitation with alginate.
abstract: beta-amylases are used in production of maltose syrup. It is shown that sweet potato beta-amylase can be purified by affinity precipitation with alginate with 80% activity yield and 44 fold purification. SDS-PAGE of the purified protein showed a single band and a subunit weight of 50 kDa. Preliminary data with soybean and barley enzymes indicate that this may be a general method for purification of beta-amylases.
authors.affiliation: Chemistry Department, Indian Institute of Technology, Hauz Khas, 110016, New Delhi, India
authors.forename: S
authors.initials: S
authors.lastname: Teotia
authors.forename: S K.
authors.initials: SK
authors.lastname: Khare
authors.forename: M N.
authors.initials: MN
authors.lastname: Gupta