A novel procedure for daily measurements of hemodynamical, hematological, and biochemical parameters in conscious unrestrained rats.
Accurate and chronic measurements of various parameters in conscious animals are fundamental for depicting pathological chronic conditions and their etiology in many experimental models, but they are often difficult to achieve. The aim of the present work was to develop and describe step-by-step a reproducible surgical procedure and daily manipulations for continuous, chronic use of conscious rats as models towards a better understanding of various cardiovascular and renal diseases and the testing of novel pharmacological drugs. The complete apparatus involved the use of a series of specialized devices (harness, rotating swivel, revolving arm) supporting a flexible, permanently implanted vascular catheter into the left femoral artery up to the abdominal aorta connected to a miniaturized individual peristaltic pump for delivering fluid at a constant rate. Such a set-up also enabled easy, quick, and reproducible daily blood sampling for the evaluation of more than 20 parameters, including the monitoring of heart rate (HR) and blood pressure in freely moving conscious rats. The overall success and survival rate reached 98% over 14 days and could be extended further. This model represents a much needed and valuable advance in surgical research techniques to evaluate the hemodynamic, hematological, biochemical, pharmacokinetic, and toxicological profile of any new drugs over time in conscious animal models such as rats. What makes this procedure satisfactory is the long-term reliable arterial access and reproducibility of the methodological approach for accurate and continuous measurements, minimizing the stress or invasiveness associated with the use of currently employed systems.
Blouin
A
A
Centre de recherche, Hôpital Laval, Institut de cardiologie et de pneumologie, Department of Medicine, Laval University, Ste-Foy, G1V 4G5, QC, Canada.
Molez
S
S
Pham
D
D
Ayach
B
B
Dussault
P
P
Escher
E
E
Jeng
A Y
AY
Battistini
B
B
D000818:Animals; D001794:Blood Pressure / Q000502:physiology; D002406:Catheterization, Peripheral / Q000295:instrumentation*; D002624:Chemistry, Clinical / Q000379:methods*; D003243:Consciousness; D015507:Drugs, Investigational / Q000493:pharmacokinetics / Q000633:toxicity; D005263:Femoral Artery; D006339:Heart Rate / Q000502:physiology; D006405:Hematology / Q000379:methods*; D006439:Hemodynamics; D008297:Male; D023421:Models, Animal; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
D015507:Drugs, Investigational