Effect of corticosteroid therapy on serum and CSF malondialdehyde and antioxidant proteins in multiple sclerosis.
OBJECTIVE
Multiple sclerosis (MS) is a disease characterised by perivascular infiltrates and demyelination of the white matter in the central nervous system. Although the precise cause of MS remains unknown, some investigations have been carried out on antioxidant mechanisms in these patients.


METHODS
In this study, malondialdehyde (MDA), as a lipid peroxidation marker, and ceruloplasmin (Cp) and transferrin (Trf), as antioxidant proteins, levels were determined in cerebrospinal fluid (CSF) and serum of 30 MS patients before and after corticosteroid therapy and in 20 control subjects. Transferrin and Cp levels were measured by the nephelometric method and MDA was measured spectrophotometrically.


RESULTS
Mean MDA(serum) and MDA(CSF) levels were found to be highest in the pretreatment group and lowest in the control group. Although there was no significant difference in terms of serum Trf level, serum Cp was found higher in pre- and posttreatment groups than in the control groups. Ceruloplasmin and Trf levels of CSF were not detectable using the nephelometric method. A significant correlation was found between MDA(CSF) and MDA(serum) in the pretreatment group (r=0.58).


CONCLUSIONS
These data revealed that lipid peroxidation was increased in serum and particulary in CSF of MS patients and was reduced with corticosteroid therapy.
Keles
M S
MS
Department of Biochemistry, Ataturk University, Faculty of Medicine, Erzurum, Turkey.
Taysi
S
S
Sen
N
N
Aksoy
H
H
Akçay
F
F
D000293:Adolescent; D000328:Adult; D000975:Antioxidants / Q000378:metabolism*; D002570:Ceruloplasmin / Q000378:metabolism; D005260:Female; D005938:Glucocorticoids / Q000008:administration & dosage*; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000097:blood* / Q000134:cerebrospinal fluid; D008775:Methylprednisolone / Q000008:administration & dosage*; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000097:blood / Q000134:cerebrospinal fluid / Q000188:drug therapy*; D014168:Transferrin / Q000378:metabolism
D000975:Antioxidants; D005938:Glucocorticoids; D014168:Transferrin; D008315:Malondialdehyde; D002570:Ceruloplasmin; D008775:Methylprednisolone