title: [Sources of error in the European Pharmacopoeia assay of halide salts of organic bases by titration with alkali].
abstract: A short overview has been given by the authors on the titrimetric assay methods of halide salts of organic bases in the pharmacopoeias of greatest importance. The alternative procedures introduced by the European Pharmacopoeia Commission some years ago to replace the non-aqueous titration with perchloric acid in the presence of mercuric acetate have also been presented and evaluated. The authors investigated the limits of applicability and the sources of systematic errors (bias) of the strongly preferred titration with sodium hydroxide in an alcoholic medium. To assess the bias due to the differences between the results calculated from the two inflexion points of the titration curves and the two real endpoints corresponding to the strong and weak acids, respectively, the mathematical analysis of the titration curve function was carried out. This bias, generally negligible when the pH change near the endpoint of the titration is more than 1 unit, is the function of the concentration, the apparent pK of the analyte and the ionic product of water (ethanol) in the alcohol-water mixtures. Using the validation data gained for the method with the titration of ephedrine hydrochloride the authors analysed the impact of carbon dioxide in the titration medium on the additive and proportional systematic errors of the method. The newly introduced standardisation procedure of the European Pharmacopoeia for the sodium hydroxide titrant to decrease the systematic errors caused by carbon dioxide has also been evaluated.
authors.affiliation: Országos Gyógyszerészeti Intézet, 1051 Budapest, Zrínyi u. 3. és Semmelweis Egyetem Gyógyszerfelügyeleti Tanszék.
authors.forename: S H
authors.initials: SH
authors.lastname: Kószeginé
authors.forename: R Z
authors.initials: RZ
authors.lastname: Ráfliné
authors.forename: T
authors.initials: T
authors.lastname: Paál
authors.forename: I
authors.initials: I
authors.lastname: Török
mesh_terms: D002245:Carbon Dioxide; D002264:Carboxylic Acids / Q000032:analysis*; D004809:Ephedrine / Q000032:analysis; D000431:Ethanol; D005060:Europe; D007202:Indicators and Reagents; D007700:Kinetics; D008628:Mercury; D010472:Perchlorates; D010602:Pharmacopoeias as Topic; D015203:Reproducibility of Results; D012492:Salts / Q000032:analysis*; D012972:Sodium Hydroxide; D017186:Titrimetry / Q000379:methods* / Q000592:standards; D014867:Water
chemical_list: D002264:Carboxylic Acids; D007202:Indicators and Reagents; D010472:Perchlorates; D012492:Salts; D014867:Water; D002245:Carbon Dioxide; D000431:Ethanol; D012972:Sodium Hydroxide; D008628:Mercury; D004809:Ephedrine; C029823:mercuric acetate