Fidelity of enzymatic ligation for DNA computing.
We describe a convenient assay for rapid qualitative evaluation of hybridization/ligation fidelity. The approach uses randomized probe strands of DNA and restriction enzyme digestion after amplification of reaction products by the polymerase chain reaction (PCR). We report ligation efficiencies and fidelities of two DNA ligases, T4 DNA ligase and Thermus aquaticus (Taq) DNA ligase, over a range of temperatures.
Faulhammer
D
D
Department of Ecology and Evolutionary Biology, Princeton University, Princeton, NJ 08544, USA.
Lipton
R J
RJ
Landweber
L F
LF
D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D011088:DNA Ligases / Q000737:chemistry / Q000378:metabolism; D004798:Enzymes / Q000737:chemistry / Q000378:metabolism*; D008954:Models, Biological; D008967:Molecular Biology / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D019914:Taq Polymerase / Q000737:chemistry / Q000378:metabolism
D004798:Enzymes; D004247:DNA; D019914:Taq Polymerase; D011088:DNA Ligases