DNA breakage due to metronidazole treatment.
The mutagenicity of metronidazole [1-(hidroxyethyl)-2-methyl-5-nitroimidazole] (MTZ) has been shown in different prokaryotic systems. However, data on human cells are still contradictory. In this study DNA damage was determined by the single cell gel electrophoresis (SCGE) assay, in lymphocytes from 10 healthy subjects treated with therapeutic doses of this drug. Samples were obtained before treatment, as well as 1 and 15 days after ending treatment. Results showed a significant increase of DNA strand breaks 1 day after ending treatment, although, an inverse correlation between the amount of DNA damage and plasma concentrations of MTZ was obtained. Thus, the observed damage may be induced by some MTZ metabolite rather than by the parent drug. Interestingly, the amount of DNA damage returned to basal levels 15 days after ending treatment, except in two individuals. This persistent damage should be further investigated.
Menéndez
D
D
Departamento de Genética y Toxicología Ambiental, Instituto de Investigaciones Biomédicas, UNAM, P.O. Box 70228, Ciudad Universitaria, 04510, México, D.F., México.
Rojas
E
E
Herrera
L A
LA
López
M C
MC
Sordo
M
M
Elizondo
G
G
Ostrosky-Wegman
P
P
D000293:Adolescent; D000328:Adult; D000890:Anti-Infective Agents / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D020552:Comet Assay; D004247:DNA / Q000187:drug effects* / Q000235:genetics; D004249:DNA Damage; D006801:Humans; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008795:Metronidazole / Q000494:pharmacology*
D000890:Anti-Infective Agents; D008795:Metronidazole; D004247:DNA