title: Microbial dehalogenation.
abstract: Novel dehalogenases have been identified recently in various bacteria that utilise halogenated substrates. X-ray studies and sequence analysis have revealed insight into the molecular mechanisms of hydrolytic dehalogenases. Furthermore, genetic and biochemical studies have indicated that reductive dehalogenases are extra-cytoplasmic corrinoid-containing iron-sulphur proteins. Sequence analysis and mutagenesis studies indicate that several dehalogenases are homologous to enzymes that carry out transformations on non-halogenated substrates.
authors.affiliation: Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, NL-9747 AG, Groningen, The Netherlands. d.b.janssen@chem.rug.nl
authors.forename: D B
authors.initials: DB
authors.lastname: Janssen
authors.forename: J E
authors.initials: JE
authors.lastname: Oppentocht
authors.forename: G J
authors.initials: GJ
authors.lastname: Poelarends
mesh_terms: D000332:Aerobiosis / Q000235:genetics / Q000502:physiology; D000693:Anaerobiosis / Q000235:genetics / Q000502:physiology; D001419:Bacteria; D018407:Bacterial Physiological Phenomena; D001673:Biodegradation, Environmental; D002384:Catalysis; D006846:Hydrocarbons, Halogenated / Q000378:metabolism / Q000633:toxicity*; D006867:Hydrolases / Q000378:metabolism*
chemical_list: D006846:Hydrocarbons, Halogenated; D006867:Hydrolases; C034507:2-haloacid dehalogenase