Expression of Moloney murine leukemia virus RNase H rescues the growth defect of an Escherichia coli mutant.
A 157-amino-acid fragment of Moloney murine leukemia virus reverse transcriptase encoding RNase H is shown to rescue the growth-defective phenotype of an Escherichia coli mutant. In vitro assays of the recombinant wild-type protein purified from the conditionally defective mutant confirm that it is catalytically active. Mutagenesis of one of the presumptive RNase H-catalytic residues results in production of a protein variant incapable of rescue and which lacks activity in vitro. Analyses of additional active site mutants demonstrate that their encoded variant proteins lack robust activity yet are able to rescue the bacterial mutant. These results suggest that genetic complementation may be useful for in vivo screening of mutant viral RNase H gene fragments and in evaluating their function under conditions that more closely mimic physiological conditions. The rescue system may also be useful in verifying the functional outcomes of mutations based on protein structural predictions and modeling.
Campbell
A G
AG
Division of Biology and Medicine, Department of Molecular Microbiology and Immunology, Brown University, Providence, RI 02912, USA. Andrew_Campbell@Brown.edu
D000595:Amino Acid Sequence; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development*; D008969:Molecular Sequence Data; D008979:Moloney murine leukemia virus / Q000201:enzymology*; D009154:Mutation; D016366:Open Reading Frames; D016914:Ribonuclease H / Q000235:genetics / Q000502:physiology*
D016914:Ribonuclease H