CaT1 expression correlates with tumor grade in prostate cancer.
Ca(2+) signaling is important for growth and survival of prostatic carcinoma (PCa) cells. Here we report that the gene for CaT1, a channel protein highly selective for Ca(2+), is expressed at high levels in human PCa and in the LNCaP PCa cell line. CaT1 mRNA levels were elevated in PCa specimens in comparison to benign prostatic hyperplasia (BPH) specimens and positively correlated with Gleason grade in a PCa series. CaT1 mRNA was suppressed by androgen and was induced by a specific androgen receptor antagonist in LNCaP cells, suggesting that the gene is negatively regulated by androgen. These findings are the first to implicate a Ca(2+) channel in PCa progression and suggest that CaT1 may be a novel target for therapy.
Peng
J B
JB
Membrane Biology Program, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts, 02115, USA.
Zhuang
L
L
Berger
U V
UV
Adam
R M
RM
Williams
B J
BJ
Brown
E M
EM
Hediger
M A
MA
Freeman
M R
MR
D000726:Androgen Antagonists / Q000494:pharmacology; D000728:Androgens / Q000378:metabolism; D000813:Anilides / Q000494:pharmacology; D001483:Base Sequence; D015220:Calcium Channels / Q000235:genetics*; D020013:Calcium Signaling; D002460:Cell Line; D017931:DNA Primers / Q000235:genetics; D004847:Epithelial Cells / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D009570:Nitriles; D011467:Prostate / Q000378:metabolism; D011470:Prostatic Hyperplasia / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012334:RNA, Neoplasm / Q000235:genetics / Q000378:metabolism; D050916:TRPV Cation Channels; D014105:Tosyl Compounds; D014407:Tumor Cells, Cultured
D000726:Androgen Antagonists; D000728:Androgens; D000813:Anilides; D015220:Calcium Channels; D017931:DNA Primers; D009570:Nitriles; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D050916:TRPV Cation Channels; C120776:TRPV6 channel; D014105:Tosyl Compounds; C053541:bicalutamide