[Isolation and culture of bovine choriocapillary endothelial cells using paramagnetic beads coated with Lycopersicon esculentum].
OBJECTIVE
To establish a pure culture of choriocapillary endothelial cells as a model of angiogenesis in vitro.


METHODS
Bovine choriocapillary endothelial cells (BCEC) were obtained by the method described by Hoffmann et al. (6) using the polystyrene paramagnetic beads coated with Lycopersicon esculentum, which attach specifically to the rest of fucose on the surface of microvascular endothelial cells. The endothelial characteristic of the cultured cells was evaluated by immunocytochemistry using anti von Willebrand factor and anti-CD 31 antibodies. Proliferation and survival of BCEC were tested using haemacytometer of Mallasez.


RESULTS
The purity of obtained BCEC culture was confirmed by positive immunocytochemical staining with anti von Willebrand and anti factor CD 31 antibodies in more than 95% of cells. The proliferation of cells in Endothelial Cell Medium resulted in twofold increase of number of cells during 4-day observation period. After reaching the confluence, the cells continued to proliferate with increase of the cell number by 60% during 4-day observation.


CONCLUSIONS
The use of paramagnetic beads coated with specific lectine provide a pure isolation of BCEC, which can be maintained in culture with preservation of their characteristic.
Swiech-Zubilewicz
A
A
Katedry i l Kliniki Okulistyki AM w Lublinie.
Soubrane
G
G
Mascarelli
F
F
D000818:Animals; D002196:Capillaries / Q000166:cytology*; D002417:Cattle; D018929:Cell Culture Techniques / Q000379:methods*; D002455:Cell Division; D002478:Cells, Cultured; D002823:Chorion / Q000098:blood supply*; D020099:Coated Materials, Biocompatible; D004730:Endothelium, Vascular / Q000166:cytology*; D007150:Immunohistochemistry; D018189:Immunomagnetic Separation; D018551:Solanum lycopersicum; D011137:Polystyrenes; D013499:Surface Properties
D020099:Coated Materials, Biocompatible; D011137:Polystyrenes