Sensitive detection of tetracyclines using europium-sensitized fluorescence with EDTA as co-ligand and cetyltrimethylammonium chloride as surfactant.
The determination of tetracyclines (TC) in aqueous solutions, based on europium-sensitized fluorescence, has been improved using EDTA as co-ligand and cetyltrimethylammonium chloride as surfactant. The method involves working in slightly alkaline solutions with the formation of a new chelate where the lanthanide ion is bound to the beta-diketone group. The method is about 6 times more sensitive than that with the Eu-TC-Triton system and LODs are 2.5 x 10(-10), 5 x 10(-10), 1.5 x 10(-9) and 2 x 10(-9) mol l-1 for TC, oxytetracycline, chlortetracycline and doxycycline, respectively. The method has been applied to the determination of TC in calf serum without sample pretreatment. The mean recovery was close to 102% and the lowest concentration attainable in serum samples was better than 0.1 microgram ml-1.
Arnaud
N
N
Laboratoire des Sciences et Stratégies Analytiques, Bâtiment CPE, Domaine Scientifique de la Doua, 69622 Villeurbanne, France.
Georges
J
J
D000818:Animals; D000900:Anti-Bacterial Agents / Q000097:blood*; D002417:Cattle; D000077286:Cetrimonium; D002593:Cetrimonium Compounds; D004492:Edetic Acid; D005063:Europium; D013050:Spectrometry, Fluorescence / Q000379:methods; D013501:Surface-Active Agents; D013754:Tetracyclines / Q000097:blood*
D000900:Anti-Bacterial Agents; D002593:Cetrimonium Compounds; D013501:Surface-Active Agents; D013754:Tetracyclines; D005063:Europium; D004492:Edetic Acid; D000077286:Cetrimonium