[Proliferation properties and telomerase activity of human embryonic tendon cells transformed by ptsA58H plasmid].
OBJECTIVE
To analysis the proliferation properties and telomerase activity of human embryonic tendon cells transformed by ptsA58H plasmid cultured in vitro continuously.


METHODS
The 40th, 70th, and 75th passages of transformed human embryonic tendon cells (THETC) were adopted. The collagen secretion of THETC was detected by immunohistochemical methods, the growth curve of different passages of THETC was compared, and chromosome karyotype was analyzed. Total RNA of THETC were extracted to detect human telomerase reverse transcriptase (hTERT) mRNA expression by RT-PCR technique.


RESULTS
When THETC were subcultured to 70 passages, the morphological characteristics of cells changed and began replicative senescence. THETC still could secret type I collagen normally. The chromosome of THETC was heteroploid (2n = 94). There were no hTERT mRNA expression.


CONCLUSIONS
SV40 transfection can not make human embryonic tendon cells immortalization, on the other hand, human embryonic tendon cells transformed by ptsA58H plasmid has no tendency of malignant transformation.
Xie
H Q
HQ
Department of Orthopedic Surgery, First University Hospital, West China University of Medical Sciences, Chengdu Sichuan, P. R. China 610041. orthop@public.cd.sc.cn
Yang
Z M
ZM
Qu
Y
Y
D002455:Cell Division; D002478:Cells, Cultured; D004268:DNA-Binding Proteins; D006801:Humans; D010957:Plasmids; D012333:RNA, Messenger / Q000378:metabolism; D019098:Telomerase / Q000032:analysis / Q000378:metabolism*; D013710:Tendons / Q000166:cytology* / Q000196:embryology; D023822:Tissue Engineering
D004268:DNA-Binding Proteins; D012333:RNA, Messenger; D019098:Telomerase