title: Polymerase chain reaction protocols for alpha globin haplotype polymorphisms.
abstract: Polymerase chain reaction protocols were designed specifically to amplify regions of the alpha globin complex that contain the nine common polymorphic haplotyping sites. These reactions provided a quicker and more sensitive approach to determining alpha globin haplotypes than Southern blotting methods.
authors.affiliation: MRC Molecular Haematology Unit, Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford, UK. kmiles@hammer.imm.ox.ac.uk
authors.forename: K L
authors.initials: KL
authors.lastname: Miles
authors.forename: J T
authors.initials: JT
authors.lastname: Norwich
authors.forename: J J
authors.initials: JJ
authors.lastname: Martinson
authors.forename: J B
authors.initials: JB
authors.lastname: Clegg
mesh_terms: D001622:Betaine; D017931:DNA Primers / Q000235:genetics; D005914:Globins / Q000235:genetics*; D006239:Haplotypes; D006801:Humans; D008274:Magnesium; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D012680:Sensitivity and Specificity
chemical_list: D017931:DNA Primers; D001622:Betaine; D005914:Globins; D008274:Magnesium