[Automated analysis for determination of PCBs in fish].
An automated analytical method for determination of polychlorinated biphenyls (PCBs) in fish was developed using supercritical fluid extraction (SFE) in combination with an automated sample preparation instrument (Prep) and GC/MS. By incorporating basic alumina with the sample in the extraction process, and optimizing the amount of carbon dioxide used, fish lipid was selectively reduced. The extract was cleaned up on a Florisil cartridge with Prep. The method was evaluated using naturally contaminated tissues and by comparison of automated analytical method results with those obtained by the conventional method. Mean recovery of PCBs from 3 kinds of fish including hairtail, mackerel and yellowtail were 69.8%, 90.2% and 81.1%, respectively. This method is less laborious and requires far less organic solvent than the conventional method, but produced comparable results.
Yasumura
K
K
Nara Prefectural Institute of Public Health, 57-6, Ohmori-cho, Nara 630-8131, Japan.
Kitamura
E
E
Uno
M
M
Tamaki
M
M
D000818:Animals; D001322:Autoanalysis / Q000379:methods*; D004785:Environmental Pollutants / Q000032:analysis*; D005399:Fishes; D011078:Polychlorinated Biphenyls / Q000032:analysis*
D004785:Environmental Pollutants; D011078:Polychlorinated Biphenyls