Structural elucidation of a capsular polysaccharide from a clinical isolate of Bacteroides vulgatus from a patient with Crohn's disease.
The structure of a capsular polysaccharide (CPS) from a clinical isolate of Bacteroides vulgatus was elucidated. B. vulgatus IMCJ 1204 was isolated from feces of a patient with Crohn's disease. CPS was prepared by phenol/water extraction of the bacterial cells followed by hydrophobic interaction chromatography and then gel filtration chromatography of the extract. The structure of CPS was determined by chemical analysis and NMR spectroscopy including DQF-COSY, TOCSY, ROESY, HSQC-TOCSY, HMQC and HMBC to be a polysaccharide composed of the following repeating unit: -->3)beta-D-Glcp(1-->6)[alpha-D-GalpNAc(1-->2)beta-D-Galp(1-->4)]beta-D-GlcpNAc(1-->3)alpha-D-Galp(1-->4)beta-D-Manp(1-->.
Hashimoto
M
M
Research Institute, International Medical Center of Japan, Tokyo, Japan.
Kirikae
F
F
Dohi
T
T
Kusumoto
S
S
Suda
Y
Y
Kirikae
T
T
D016667:Bacterial Capsules; D001439:Bacteroides / Q000737:chemistry / Q000302:isolation & purification* / Q000472:pathogenicity; D001442:Bacteroides Infections / Q000382:microbiology*; D002240:Carbohydrate Sequence; D003424:Crohn Disease / Q000382:microbiology*; D005243:Feces / Q000382:microbiology*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008969:Molecular Sequence Data; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000302:isolation & purification
D011135:Polysaccharides, Bacterial; C431064:capsular polysaccharide, Bacteroides vulgatus