title: Heparin inhibits retrovirus binding to fibronectin as well as retrovirus gene transfer on fibronectin fragments.
abstract: Fibronectin fragments have been shown to improve retrovirus gene transfer efficiency by binding retrovirus and target cells. Using a novel virus adhesion assay, we confirmed binding of type C oncoretrovirus vectors to the heparin II domain of fibronectin and demonstrated inhibition of viral binding and gene transfer by heparin.
authors.affiliation: Max Delbrück Center for Molecular Medicine, Berlin, Germany. dirk.carstanjen@berlin.de
authors.forename: D
authors.initials: D
authors.lastname: Carstanjen
authors.forename: P
authors.initials: P
authors.lastname: Dutt
authors.forename: T
authors.initials: T
authors.lastname: Moritz
mesh_terms: D018952:Antigens, CD34 / Q000032:analysis; D002499:Centrifugation, Density Gradient; D005353:Fibronectins / Q000502:physiology*; D022761:Gene Transfer, Horizontal; D006412:Hematopoietic Stem Cells / Q000378:metabolism; D006493:Heparin / Q000494:pharmacology*; D008715:Methionine / Q000378:metabolism; D012190:Retroviridae / Q000187:drug effects* / Q000235:genetics / Q000502:physiology
chemical_list: D018952:Antigens, CD34; D005353:Fibronectins; D006493:Heparin; D008715:Methionine