Utilization of an alternative carbon source for efficient production of human alpha(1)-antitrypsin by genetically engineered rice cell culture.
Human alpha(1)-antitrypsin was produced by genetically engineered rice cells using promoter and signal peptide of a rice alpha-amylase isozyme. Batch and continuous cultures were employed to investigate the effects of alternative carbon sources on the alpha(1)-antitrypsin production. While this expression system is inducible by sugar depletion, we have found that the productivity of alpha(1)-antitrypsin increased 2.4- to 3.4-fold, compared with the control medium without carbon source, in medium containing an alternative carbon source, such as pyruvic acid and glyoxylic acid. The accumulated alpha(1)-antitrypsin in the medium containing pyruvic acid reached 18.2-24.2 mg/g-dry cell in 50-70 h by batch culture.
Terashima
M
M
Department of Chemical Engineering, Graduate School of Engineering, Osaka Prefecture University, 1-1, Gakuen-cho, Sakai, 599-8531, Osaka Japan. terasima@chemeng.osakafu-u.ac.jp
Ejiri
Y
Y
Hashikawa
N
N
Yoshida
H
H
D002244:Carbon / Q000378:metabolism*; D002478:Cells, Cultured; D005818:Genetic Engineering; D006801:Humans; D008353:Mannitol / Q000378:metabolism; D012275:Oryza / Q000235:genetics* / Q000378:metabolism; D015202:Protein Engineering / Q000379:methods*; D019289:Pyruvic Acid / Q000378:metabolism*; D000515:alpha 1-Antitrypsin / Q000235:genetics* / Q000378:metabolism; D000516:alpha-Amylases / Q000235:genetics / Q000378:metabolism
D000515:alpha 1-Antitrypsin; D008353:Mannitol; D002244:Carbon; D019289:Pyruvic Acid; D000516:alpha-Amylases