title: Isolation, characterization and quantification of microcystins (heptapeptides hepatotoxins) in Microcystis aeruginosa dominated bloom of Lalla Takerkoust lake-reservoir (Morocco).
abstract: This paper presents the first data on the identification, characterization and quantification of microcystins isolated from both an extract of a cyanobacteria natural bloom, collected from a eutrophic Moroccan reservoir (Lalla Takerkoust, Marrakesh) and an isolated strain cultivated under laboratory conditions. The isolation and purification of toxins was performed by reverse phase HPLC and then characterized by amino acid analysis and fast atom bombardment mass spectrometry (FAB-MS). Chemical characterization of the toxins from the bloom revealed variants of microcystins such as Mcyst-LR, Mcyst-RR, Mcyst-YR and [D-Asp3]Mcyst-LR. However, the Microcystis aeruginosa strain produced only Mcyst-RR. Using an ELISA assay the total microcystin contents of eight bloom samples collected from 1994 to 1997 ranged from 0.7 to 8.8 microg/mg of lyophilized material. The two isolated Microcystis strains contained higher amounts of microcystins (0.65 microg/ mg of dry weight) than the Pseudanabaena strains (0.021 microg/mg of dry weight). Our results show that the presence of cyanobacteria toxins in water used for drinking in a North African country may be regarded as an health hazard. These results are a contribution to the knowledge of the biogeography of toxic cyanobacteria and their toxins, namely in north African countries.
authors.affiliation: University Cadi Ayyad, Faculty of Sciences Marrakesh Semlalia PO Box. S/2390 Department of Biology, 40 000, Marrakesh, Morocco.
authors.forename: B
authors.initials: B
authors.lastname: Oudra
authors.forename: M
authors.initials: M
authors.lastname: Loudiki
authors.forename: B
authors.initials: B
authors.lastname: Sbiyyaa
authors.forename: R
authors.initials: R
authors.lastname: Martins
authors.forename: V
authors.initials: V
authors.lastname: Vasconcelos
authors.forename: N
authors.initials: N
authors.lastname: Namikoshi
mesh_terms: D001427:Bacterial Toxins / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification; D005618:Fresh Water / Q000382:microbiology*; D007928:Lethal Dose 50; D052998:Microcystins; D046931:Microcystis / Q000737:chemistry*; D008970:Molecular Weight; D009018:Morocco; D010456:Peptides, Cyclic / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013056:Spectrophotometry, Ultraviolet; D014871:Water Microbiology
chemical_list: D001427:Bacterial Toxins; D052998:Microcystins; D010456:Peptides, Cyclic; C078588:microcystin