title: Relationship between outcome of proliferative vitreoretinopathy and results of tissue culture of excised preretinal membranes.
abstract: The purpose of this study was to explore the relationship between clinical post-surgery outcome of proliferative vitreoretinopathy (PVR) and the laboratory results of tissue culture, the specimens of which were excised of pre- or subretinal membranes from PVR patients. Surgically excised membranes from 25 PVR patients were microdissected into small pieces and plated into culture dishes with F12 medium supplemented with 30% fetal bovine serum. After primary culture became confluent, cells were passaged in subculture with F12 medium (10% fetal bovine serum). PVR patients were followed-up after surgery for an average of 21 months. The clinical outcome was compared, according to the growth pattern of the cells derived from the explanted membranes. In 25 PVR patients, 16 cases showed cell migration in the membrane, and cells grew rapidly to confluence in the primary culture in 7 cases. All active growing cells were identified as retinal pigment epithelial (RPE) cells by immunocytochemistry. In 7 cases with active cell growth, all had recurrent retinal detachment. In 18 cases without active cell growth, only 4 cases had the same outcomes. Statistical study showed that the difference between these two groups was significant (P < 0.01). These results indicate that the growth capacity of cultured RPE derived from excised membranes of PVR patients strongly influenced the prognosis for surgery.
authors.affiliation: Department of Ophthalmology, Kaohsiung Medical University, No. 100, Shih-Chuan 1st Road, Kaohsiung, Taiwan.
authors.forename: W C
authors.initials: WC
authors.lastname: Wu
authors.forename: Y H
authors.initials: YH
authors.lastname: Kao
authors.forename: D N
authors.initials: DN
authors.lastname: Hu
mesh_terms: D002455:Cell Division; D046508:Culture Techniques; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D006801:Humans; D010857:Pigment Epithelium of Eye / Q000473:pathology*; D011379:Prognosis; D018630:Vitreoretinopathy, Proliferative / Q000473:pathology* / Q000601:surgery
chemical_list: D005904:Glial Fibrillary Acidic Protein