Utility of HHV8 RNA detection for differentiating Kaposi's sarcoma from its mimics.
BACKGROUND
The diagnostic distinction of atypical vascular lesion or angiomatoid/hemosiderotic dermatofibroma and Kaposi's sarcoma can be difficult, especially in AIDS patients. Given the strong association between human herpes virus 8 (HHV8) and Kaposi's sarcoma, this study attempts to determine if HHV8 RNA detection by reverse transcription (RT) in situ polymerase chain reaction (PCR) could help in differentiating these entities.


METHODS
Twenty-three Kaposi's sarcoma cases, 13 dermatofibromas, including 7 angiomatoid or hemosiderotic variants and eight atypical vascular lesions were tested for HHV8 by RT in situ PCR; five of the patients in the latter two groups were known to have AIDS.


RESULTS
HHV8 RNA was detected in each of the 23 cases considered on histologic grounds to represent Kaposi's sarcoma; viral RNA and DNA localized to the majority of the endothelial and stromal spindle cells. None of the cases of dermatofibroma were HHV8 positive, whereas two of the atypical vascular lesions were viral positive, leading to a final diagnosis of Kaposi's sarcoma.


CONCLUSIONS
RT in situ PCR for HHV8 RNA is a useful tool in the differential diagnosis between Kaposi's sarcoma and its mimics.
Nuovo
M
M
Department of Pathology, Ohio State University Medical Center, Columbus, Ohio 43210-1228, USA.
Nuovo
G
G
D000163:Acquired Immunodeficiency Syndrome / Q000150:complications; D000783:Aneurysm / Q000175:diagnosis; D000798:Angiomatosis / Q000175:diagnosis; D001808:Blood Vessels / Q000473:pathology; D003937:Diagnosis, Differential; D015497:HIV-1 / Q000235:genetics; D006486:Hemosiderosis / Q000175:diagnosis; D019288:Herpesvirus 8, Human / Q000235:genetics / Q000302:isolation & purification*; D018219:Histiocytoma, Benign Fibrous / Q000175:diagnosis* / Q000821:virology; D006801:Humans; D009389:Neovascularization, Pathologic / Q000473:pathology; D012367:RNA, Viral / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012514:Sarcoma, Kaposi / Q000175:diagnosis* / Q000821:virology; D012878:Skin Neoplasms / Q000175:diagnosis* / Q000821:virology
D012367:RNA, Viral