title: Development of a methanogenic process to degrade exhaustively the organic fraction of municipal "grey waste" under thermophilic and hyperthermophilic conditions.
abstract: Different laboratory-scale, continuously driven reactor concepts (up to 3 reactors in series, max. 70 degrees C) for anaerobic digestion of the organic fraction of municipal grey waste were investigated. Over a period of 2 1/2 years several setups of reactors being daily fed and held in steady state balance were investigated. The perferred variant was a 2-stage setup with a HRT of 4.3 d for the 1st and 14.2 d for the 2nd reactor. Removal efficiencies of VS obtained by comparing the organic loading rate (OLR, g VS/l/d) of the effluent with the OLR of the feed could reach 80%. Removal efficiencies determined indirectly by the combined biogas yield of the 1st and 2nd reactor stage revealed even up to 91.5% of the theoretical possible yield of 807 l/kg VS. The produced gas had a methane content of 60-65%. A completely distinct hydrolysis stage with a gas production of only 1.6-5.5% of the theoretical yield could be reached by hyperthermophilic conditions (60-70 degrees C) or by a HRT of 1.25 d. It also demonstrated that a stable methanogenesis was not possible at temperatures of 60-70 degrees C. Kinetic analyses of the 2nd reactor stage revealed that the degradation of VS fell from 80 to 40% with raising organic loading rate (OLR) from 3 to 11 g VS/l/d. In contrast to this the VS-removal of the first hydrolysis reactor stage increased linearily from 5 to 20% at raising OLR's from 12 to 26 g VS/l/d. The same kinetics with linear increase exhibited the specific cellulose degradation with conversion rates of 0.1-3 x 109 g cellulose/single bacterium (10(-12) g)/d. This was an indication for the cellulose degradation as a rate limiting step. Both reactor stages combined allowed an optimal VS removal efficiency at OLR of 10 g VS/l/d. Analysis of bacterial populations of 28 reactors were referred either to eubacteria utilizing different sugars or cellulose or acetate or H2-CO2 or archaea (plus antibiotics) with acetate or H2-CO2 as substrate. H2-CO2 utilizers with numbers of 10(8)-10(10)/g TS dominated obviously the acetotrophic methanogens by the factor 10-10,000. This explained the observed short HRTs being possible.
authors.affiliation: University of Applied Sciences Hamburg, D-21033 Hamburg-Bergedorf, Germany.
authors.forename: P A
authors.initials: PA
authors.lastname: Scherer
authors.forename: G R
authors.initials: GR
authors.lastname: Vollmer
authors.forename: T
authors.initials: T
authors.lastname: Fakhouri
authors.forename: S
authors.initials: S
authors.lastname: Martensen
mesh_terms: D001421:Bacteria, Anaerobic / Q000378:metabolism*; D001673:Biodegradation, Environmental; D019149:Bioreactors / Q000382:microbiology / Q000592:standards; D005232:Fatty Acids, Volatile / Q000032:analysis; D005858:Germany; D006358:Hot Temperature; D017745:Incineration / Q000379:methods*; D008697:Methane / Q000378:metabolism; D000644:Quaternary Ammonium Compounds / Q000032:analysis; D012037:Refuse Disposal / Q000379:methods*
chemical_list: D005232:Fatty Acids, Volatile; D000644:Quaternary Ammonium Compounds; D008697:Methane