title: Purification of Piscirickettsia salmonis and associated phage particles.
abstract: Piscirickettsia salmonis was isolated from cell culture using differential centrifugation and purified on a 30% Percol gradient. The purity of the preparation was assessed by transmission electron microscopy and phage-like particles were found to be associated with some of the P. salmonis isolates examined. This is believed to be the first report of a phage associated with rickettsia from fish.
authors.affiliation: Institute of Aquaculture, University of Stirling, Scotland, UK.
authors.forename: S A
authors.initials: SA
authors.lastname: Yuksel
authors.forename: K D
authors.initials: KD
authors.lastname: Thompson
authors.forename: A E
authors.initials: AE
authors.lastname: Ellis
authors.forename: A
authors.initials: A
authors.lastname: Adams
mesh_terms: D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient / Q000662:veterinary; D019084:Fluorescent Antibody Technique, Indirect / Q000662:veterinary; D020563:Gammaproteobacteria / Q000302:isolation & purification* / Q000472:pathogenicity; D008854:Microscopy, Electron / Q000662:veterinary; D012281:Rickettsia / Q000302:isolation & purification*; D012474:Salmon / Q000821:virology