title: Ultraviolet-B radiation induces modulation of antigen presentation of herpes simplex virus by human epidermal cells.
abstract: Although ultraviolet (UV) B radiation is known to be immunosuppressive, there is little information regarding a relevant immunological endpoint to assess human subjects in vivo. Therefore, we have examined the effect of in vivo UV radiation on the ability of human epidermal cells (EC) to present herpes simplex virus (HSV) antigens to memory T cells. Human volunteers, who were seropositive for HSV, were exposed to one minimal erythemal dose (MED) for four consecutive days. EC, prepared from suction blister roofs, were co-cultured with autologous T cells in the presence of HSV. HSV antigen presentation by UV-exposed EC was increased compared with control, nonexposed EC. This up-regulation correlated with an influx of macrophages into the epidermis, which are considered to be associated with UV-induced tolerance. Altering the UV protocol to a sub-erythemal UV dose for four consecutive days or to a single high dose of 2 MED, resulted in suppressed HSV antigen presentation, without the influx of the UV-macrophages. One of the goals of the present study was to eventually use this HSV system to investigate sunscreen immunoprotection. A pilot study with a TiO2-containing sunscreen suggested that the endpoint for UV-induced immunosuppression presented here is promising to be used for human in vivo sunscreen immunoprotection studies.
authors.affiliation: Center for Electron Microscopy, Leiden University Medical Center, Leiden, The Netherlands. RG.Vandermolen@ed.ac.uk
authors.forename: R G
authors.initials: RG
authors.lastname: van der Molen
authors.forename: C
authors.initials: C
authors.lastname: Out-Luiting
authors.forename: F H
authors.initials: FH
authors.lastname: Claas
authors.forename: M
authors.initials: M
authors.lastname: Norval
authors.forename: H K
authors.initials: HK
authors.lastname: Koerten
authors.forename: A M
authors.initials: AM
authors.lastname: Mommaas
mesh_terms: D000328:Adult; D017951:Antigen Presentation / Q000276:immunology / Q000528:radiation effects*; D018949:Antigens, CD1 / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology*; D002455:Cell Division; D004307:Dose-Response Relationship, Radiation; D000078404:Epidermal Cells; D004817:Epidermis / Q000187:drug effects / Q000276:immunology / Q000528:radiation effects*; D006684:HLA-DR Antigens / Q000276:immunology; D018259:Herpesvirus 1, Human / Q000276:immunology*; D006801:Humans; D007801:Langerhans Cells / Q000166:cytology; D008264:Macrophages / Q000276:immunology; D008875:Middle Aged; D013473:Sunscreening Agents / Q000494:pharmacology; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology; D013997:Time Factors; D014025:Titanium / Q000494:pharmacology; D014466:Ultraviolet Rays; D014838:Volunteers
chemical_list: D018949:Antigens, CD1; D000956:Antigens, Viral; C419393:CD1a antigen; D006684:HLA-DR Antigens; D013473:Sunscreening Agents; C009495:titanium dioxide; D014025:Titanium