Vaxfectin enhances antigen specific antibody titers and maintains Th1 type immune responses to plasmid DNA immunization.
Antigen specific immune responses were characterized after intramuscular immunization of BALB/c mice with 5 antigen encoding plasmid DNAs (pDNAs) complexed with Vaxfectin, a cationic lipid formulation. Vaxfectin increased IgG titers for all of the antigens with no effect on the CTL responses to the 2 antigens for which CTL assays were performed. Both antigen specific IgG1 and IgG2a were increased, although IgG2a remained greater than IgG1. Furthermore, Vaxfectin had no effect on IFN-gamma or IL-4 production by splenocytes re-stimulated with antigen, suggesting that the Th1 type responses typical of intramuscular pDNA immunization were not altered. Studies with IL-6 -/- mice suggest that the antibody enhancement is IL-6 dependent and results in a correlative increase in antigen specific antibody secreting cells.
Reyes
L
L
Department of Cell Biology, Vical Incorporated, 9373 Towne Centre Dr., Suite 100, San Diego, CA 92121, USA.
Hartikka
J
J
Bozoukova
V
V
Sukhu
L
L
Nishioka
W
W
Singh
G
G
Ferrari
M
M
Enas
J
J
Wheeler
C J
CJ
Manthorpe
M
M
Wloch
M K
MK
D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000818:Animals; D000918:Antibody Specificity / Q000276:immunology*; D016207:Cytokines / Q000096:biosynthesis; D004337:Drug Carriers; D004983:Ethanolamines; D005260:Female; D007111:Immunity, Cellular / Q000276:immunology; D007074:Immunoglobulin G / Q000096:biosynthesis; D007273:Injections, Intramuscular; D015850:Interleukin-6 / Q000172:deficiency / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D009227:Myristic Acids; D010957:Plasmids / Q000008:administration & dosage / Q000276:immunology*; D018417:Th1 Cells / Q000276:immunology*
D000276:Adjuvants, Immunologic; D016207:Cytokines; D004337:Drug Carriers; D004983:Ethanolamines; D007074:Immunoglobulin G; D015850:Interleukin-6; D009227:Myristic Acids