Human corneal epithelial extracellular matrix perlecan serves as a site for Pseudomonas aeruginosa binding.
OBJECTIVE
Previous data has shown that basement membrane associated perlecan serves as a binding site for Pseudomonas aeruginosa in the wounded mouse cornea. The current study determined whether it also provides a binding site for Pseudomonas aeruginosa in transformed human corneal epithelium.


METHODS
Bacterial adherence to transformed human corneal epithelial cells grown in normal or in media containing various inhibitors of glycosaminoglycan synthesis was tested. Bacterial binding was similarly tested in wild-type and in mutant Chinese hamster ovary cell lines naturally deficient in glycosaminoglycan synthesis. Transformed human corneal epithelial extracellular matrix also was tested before and after treatment with anti-proteoglycan monoclonal antibodies or heparinase III before bacterial inoculation. Scanning electron microscopy was used to quantitate adherent bacteria. Intact transformed human corneal epithelial cells or extracellular matrix, the latter either treated or not treated with heparinase III or chondroitin ABC lyase were stained to localize perlecan.


RESULTS
Examination of the binding of bacteria to transformed human corneal epithelial cells (normal media vs with inhibitors) and Chinese hamster ovary cell lines suggested that bacterial binding was not associated with the surface of either cell type. In contrast, anti-perlecan antibody, as well as heparinase III decreased the binding of bacteria to corneal extracellular matrix. Fluorescence staining localized perlecan to the extracellular matrix beneath the corneal epithelial cells.


CONCLUSIONS
Perlecan localized to the extracellular matrix but not the apical surface of transformed human corneal epithelial cells, provides a binding site for Pseudomonas aeruginosa.
Chen
L
L
Department of Anatomy/Cell Biology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA.
Hazlett
L D
LD
D000818:Animals; D000911:Antibodies, Monoclonal; D001422:Bacterial Adhesion; D016466:CHO Cells / Q000382:microbiology; D002478:Cells, Cultured; D006224:Cricetinae; D019573:Epithelium, Corneal / Q000378:metabolism* / Q000382:microbiology* / Q000648:ultrastructure; D005109:Extracellular Matrix / Q000378:metabolism* / Q000382:microbiology* / Q000648:ultrastructure; D005136:Eye Proteins / Q000378:metabolism; D019084:Fluorescent Antibody Technique, Indirect; D019812:Heparan Sulfate Proteoglycans / Q000378:metabolism*; D006801:Humans; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D011550:Pseudomonas aeruginosa / Q000502:physiology*
D000911:Antibodies, Monoclonal; D005136:Eye Proteins; D019812:Heparan Sulfate Proteoglycans; C071927:perlecan