Perturbation of the nucleus: a novel Hog1p-independent, Pkc1p-dependent consequence of hypertonic shock in yeast.
Hypertonic shock of Saccharomyces cerevisiae activates the Hog1p MAP kinase cascade. In contrast, protein kinase C (Pkc1p) and the "cell integrity" MAP kinase cascade are critical for the response to hypotonic shock. We observed that hypertonic shock transiently relocated many, but not all, nuclear and nucleolar proteins to the cytoplasm. We hypothesized that the relocation of nuclear proteins was due to activation of the Hog1p kinase cascade, yet, surprisingly, Hog1p was not required for these effects. In contrast, Pkc1p kinase activity was required, although the Pkc1p MAP kinase cascade and several factors known to lie upstream and downstream of Pkc1p were not. Moreover, sudden induction of a hyperactive form of Pkc1p was sufficient to relocate nuclear proteins. Taken together, these observations show that the scope of involvement of Pkc1p in the organization of the nucleus considerably exceeds what has been characterized previously. The relocation of nuclear proteins is likely to account for the profound inhibition of RNA synthesis that was observed during hypertonic shock.
Nanduri
J
J
Pathology Department and Cell Biology Program, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106, USA.
Tartakoff
A M
AM
D002467:Cell Nucleus / Q000201:enzymology / Q000378:metabolism*; D005656:Fungal Proteins / Q000378:metabolism*; D006982:Hypertonic Solutions; D015151:Immunoblotting; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D011493:Protein Kinase C; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins; D015398:Signal Transduction; D013696:Temperature; D013997:Time Factors
D005656:Fungal Proteins; D006982:Hypertonic Solutions; D029701:Saccharomyces cerevisiae Proteins; C097993:Pkc1 protein, Trichoderma reesei; D011493:Protein Kinase C; C088589:HOG1 protein, S cerevisiae; D020928:Mitogen-Activated Protein Kinases