Screening phage-displayed combinatorial peptide libraries.
Among the many techniques available to investigators interested in mapping protein-protein interactions is phage display. With a modest amount of effort, time, and cost, one can select peptide ligands to a wide array of targets from phage-display combinatorial peptide libraries. In this article, protocols and examples are provided to guide scientists who wish to identify peptide ligands to their favorite proteins.
Kay
B K
BK
Department of Pharmacology, University of Wisconsin-Madison, 1300 University Avenue, Madison, Wisconsin 53706-1532, USA. bkkay@facstaff.wisc.edu
Kasanov
J
J
Yamabhai
M
M
D020650:Combinatorial Chemistry Techniques; D004247:DNA / Q000138:chemical synthesis; D004797:Enzyme-Linked Immunosorbent Assay; D006863:Hydrogen-Ion Concentration; D019151:Peptide Library; D010957:Plasmids / Q000737:chemistry / Q000235:genetics; D011485:Protein Binding / Q000276:immunology*; D017422:Sequence Analysis, DNA
D019151:Peptide Library; D004247:DNA