title: Immunocytochemical localization of different cell types in the adenohypophysis of the cichlid fish Cichlasoma dimerus (Heckel, 1840).
abstract: The adenohypophysis of the cichlid fish Cichlasoma dimerus was studied using the avidin-biotin-peroxidase method with antisera raised against piscine pituitary hormones and heterologous antisera against mammalian pituitary hormones. Antiserum raised against rabbit ACTH recognized a group of cells bordering the neurohypophysis (NH) in the rostral pars distalis (RPD). Anti-chum salmon prolactin (PRL) identified a compact group of cells in the periphery of the RPD. Gonadotropin II (GTH II), thyrotropin (TSH) and growth hormone (GH)-ir cells were localized in the proximal pars distalis. Ir-GTH II cells were also observed in the dorsal area of the pars intermedia (PI). Ir-GTH I cells could not be identified using anti-chum salmon GTH I, this may be due either to a failure of the antisera to recognize the gonadotropin or to a low expression of the hormone in adults of this species. PAS positive cells from the PI bound specifically with three different antisera raised against somatolactin (SL) of four different fish species. These cells surrounded deep branches of the NH in the PI.
authors.affiliation: Laboratorio de Embriología Animal, Dpto. de Ciencias Biológicas, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, (C1428EHA), Buenos Aires, Argentina. pandolfi@bg.fcen.uba.ar
authors.forename: M
authors.initials: M
authors.lastname: Pandolfi
authors.forename: D A
authors.initials: DA
authors.lastname: Paz
authors.forename: C
authors.initials: C
authors.lastname: Maggese
authors.forename: F J
authors.initials: FJ
authors.lastname: Meijide
authors.forename: P G
authors.initials: PG
authors.lastname: Vissio
mesh_terms: D000324:Adrenocorticotropic Hormone / Q000032:analysis; D000818:Animals; D029941:Fish Proteins; D006023:Glycoproteins / Q000032:analysis; D006062:Gonadotropins / Q000032:analysis; D013006:Growth Hormone / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D009474:Neurons / Q000166:cytology; D010471:Perches / Q000033:anatomy & histology*; D010903:Pituitary Gland, Anterior / Q000737:chemistry* / Q000166:cytology*; D010907:Pituitary Hormones / Q000032:analysis; D011388:Prolactin / Q000032:analysis; D013972:Thyrotropin / Q000032:analysis
chemical_list: D029941:Fish Proteins; D006023:Glycoproteins; D006062:Gonadotropins; D010907:Pituitary Hormones; C064448:somatolactin protein, fish; D000324:Adrenocorticotropic Hormone; D011388:Prolactin; D013972:Thyrotropin; D013006:Growth Hormone