title: Determination of a new oral iron chelator, ICL670, and its iron complex in plasma by high-performance liquid chromatography and ultraviolet detection.
abstract: ICL670 is a representative of a new class of orally active tridentate selective iron chelators. Two molecules of ICL670 are required to form a complete hexacoordinate chelate Fe-[ICL670]2 with one ferric iron. A simple and rapid HPLC-UV method for the separate determination of ICL670 and Fe-[ICL670]2 in the plasma of iron-overloaded patients is described. Plasma samples were prepared as rapidly as possible, the tubes being kept at 4 degrees C. Plasma proteins were precipitated with methanol. The supernatant was diluted with water and placed on the refrigerated sample rack of an autosampler before injection. The chromatographic separations were achieved on an Alltima C18 column using 0.05 M Na2HPO4 and 0.01 M tetrabutylammonium hydrogen sulfate-acetonitrile-methanol (41:9:50, v/v/v) as mobile phase. The analytes were detected at 295 nm. Calibration and quality control samples were prepared in normal human plasma. The mean accuracy (n=6) over the entire investigated concentration range 0.25-20 microg/ml ranged from 91 to 109% with a coefficient of variation (C.V.) from 4 to 8% for ICL670, and from 95 to 105% with a C.V. from 2 to 20% for the iron complex. The dissociation of the complex during analysis was shown to be marginal. The iron removal from plasma of iron-overloaded patients by free ICL670 during analysis was low. The in vitro iron transfer from the iron pools of iron-overloaded plasma onto ICL670 was shown to be a slow process.
authors.affiliation: Drug Metabolism and Pharmacokinetics, Novartis Pharma SA, Rueil-Malmaison, France.
authors.forename: M C
authors.initials: MC
authors.lastname: Rouan
authors.forename: F
authors.initials: F
authors.lastname: Marfil
authors.forename: P
authors.initials: P
authors.lastname: Mangoni
authors.forename: R
authors.initials: R
authors.lastname: Séchaud
authors.forename: H
authors.initials: H
authors.lastname: Humbert
authors.forename: G
authors.initials: G
authors.lastname: Maurer
mesh_terms: D001565:Benzoates / Q000032:analysis*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D000077588:Deferasirox; D000077543:Deferiprone; D003676:Deferoxamine / Q000737:chemistry; D004355:Drug Stability; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007501:Iron / Q000097:blood*; D007502:Iron Chelating Agents / Q000032:analysis*; D019190:Iron Overload / Q000097:blood; D015394:Molecular Structure; D011728:Pyridones / Q000737:chemistry; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature; D013789:Thalassemia / Q000097:blood; D014230:Triazoles / Q000032:analysis*
chemical_list: D001565:Benzoates; D007502:Iron Chelating Agents; D011728:Pyridones; D014230:Triazoles; D000077543:Deferiprone; D007501:Iron; D003676:Deferoxamine; D000077588:Deferasirox