Cloning, sequencing and expression in the seeds and radicles of two Lupinus albus conglutin gamma genes.
Two genes encoding conglutin gamma have been isolated from a Lupinus albus genomic library and sequenced. The expression of conglutin gamma was studied by partial amino acid sequencing of the mature seed protein and by nucleotide sequencing of reverse transcriptase-polymerase chain reaction products from various tissues during the plant life cycle.
Scarafoni
A
A
Dipartmento di Scienze Molecolari Agroalimentari, Università degli Studi di Milano, Milan, Italy. alessio.scarafoni@unimi.it
Di Cataldo
A
A
Vassilevskaia
T D
TD
Bekman
E P
EP
Rodrigues-Pousada
C
C
Ceciliani
F
F
Duranti
M
M
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D015698:Genomic Library; D018525:Germination; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D018517:Plant Roots / Q000378:metabolism; D012639:Seeds / Q000378:metabolism; D016415:Sequence Alignment
D010940:Plant Proteins; C079483:conglutin-gamma protein, Lupinus angustifolius