[Persistence of type 1 human immunodeficiency virus (HIV-1) in MT-4 cells in presence of alpha-interferon].
The development of HIV infection during serial passages of HIV-1 strains with different replication capacity was studied in the presence of natural human alpha-interferon (IFN-alpha). The virus activity was evaluated by accumulation of virus-specific proteins in cells and culture fluid, determined by Western blot method and analysis of viral DNA in the cells by polymerase chain reaction. IFN-alpha suppressed replication of HIV-1/IIIB and 1974. The replication of strain 1974 (with a 10-fold lower replicative activity than strain IIIB) was inhibited during the first passage and of strain IIIb during the second passage. After the fourth passage IFN-2 alpha completely suppressed the replication of strain 1974, and the virus activity did not manifest after 4 consecutive co-culturings of cells from fourth viral passage with intact cells without IFN-alpha. A population of viral particles whose replicative activity was virtually undetected in the presence of IFN-alpha seemed to persist during serial passages of strain IIIB in MT-4 cells. On the other hand, the virus replication was restored during the first co-culturing of cells from the virus subculture with intact cells in the absence of IFN-alpha. Presumably, an increase in the level of IFN-alpha in the blood of HIV-infected patients at late stages of AIDS is not accidental and is caused by appearance of more virulent variants of the virus.
Selimova
L M
LM
Iordanskiĭ
S N
SN
Kazennova
E V
EV
Bobkov
A V
AV
D000998:Antiviral Agents / Q000494:pharmacology*; D002461:Cell Line, Transformed; D018920:Coculture Techniques; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D016898:Interferon-alpha / Q000494:pharmacology* / Q000502:physiology; D013601:T-Lymphocytes / Q000166:cytology / Q000821:virology; D014779:Virus Replication / Q000187:drug effects
D000998:Antiviral Agents; D016898:Interferon-alpha