Quenching of biotinylated aequorin bioluminescence by dye-labeled avidin conjugates: application to homogeneous bioluminescence resonance energy transfer assays.
[see reaction]. Avidin conjugates containing the covalently attached dyes QSY-7 and dabcyl were prepared and shown to quench the bioluminescence of biotinylated aequorin. Quenching efficiency was shown to be dependent on both the label-to-avidin ratio and the concentration of the avidin conjugate. These properties were exploited to develop a homogeneous bioluminescence resonance energy transfer (BRET) assay for biotin.
Adamczyk
M
M
Department of Chemistry, Diagnostics Division, Abbott Laboratories, 100 Abbott Park Road, Abbott Park, Illinois 60064-6016, USA. maciej.adamczyk@abbott.com
Moore
J A
JA
Shreder
K
K
D000331:Aequorin / Q000737:chemistry*; D001360:Avidin / Q000737:chemistry*; D001710:Biotin / Q000737:chemistry*; D004735:Energy Transfer; D008163:Luminescent Measurements
D001360:Avidin; D000331:Aequorin; D001710:Biotin