Inexpensive purification of P450 reductase and other proteins using 2',5'-adenosine diphosphate agarose affinity columns.
Two reductases, P450 oxidoreductase and P450Bm-3 reductase, were purified on a 2',5'-adenosine diphosphate solid support. Although the efficiency of these columns is well established, the cost of the resin and the eluting material 2'-adenosine can be prohibitive. Herein we show that the less costly 2',3'-adenosine monophosphate is an excellent eluting material.
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Department of Chemistry, Washington State University, Pullman, WA 99164-4630, USA. DAR73@mailcity.com
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Jones
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D000244:Adenosine Diphosphate / Q000378:metabolism; D000249:Adenosine Monophosphate / Q000378:metabolism; D001426:Bacterial Proteins; D002846:Chromatography, Affinity; D002847:Chromatography, Agarose; D003577:Cytochrome P-450 Enzyme System / Q000302:isolation & purification* / Q000378:metabolism; D006899:Mixed Function Oxygenases / Q000302:isolation & purification* / Q000378:metabolism; D009251:NADPH-Ferrihemoprotein Reductase / Q000302:isolation & purification* / Q000378:metabolism; D011485:Protein Binding
D001426:Bacterial Proteins; C045716:adenosine 2',5'-diphosphate; D000249:Adenosine Monophosphate; D000244:Adenosine Diphosphate; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases; D009251:NADPH-Ferrihemoprotein Reductase; C052423:flavocytochrome P450 BM3 monoxygenases