title: Presence of proliferating (MiB-1-positive) cells in cervical smears of women infected with HIV is associated with clinical outcome: a study of Brazilian women.
abstract: The hypothesis tested was that there is an association between the presence of proliferating (MiB-1-positive) cervical cells and clinical outcome of women infected with human immunodeficiency virus (HIV). Female partners (attending the Gynecology Outpatients Clinic of the University Hospital of Rio Grande, Brazil) of known HIV-positive (HIV+) men were used for this pilot study. Among these women, 25 were also HIV+. Papanicolaou smears of these 25 HIV+ women and of 44 HIV- women were graded as negative, CIN I, CIN II, or CIN III, using neural network screening. MiB-1 grading and HPV identification were also performed. The immune status of patients was determined using the current Centers for Disease Control classification. In agreement with the scientific literature, in these Brazilian women both CIN and HPV were associated with HIV. In the HIV+ women, the immune status tends to correlate with MiB-1 grading. Also, in the one case in whom progression from CIN I to invasive cervical carcinoma was observed, the smear contained many MiB-1-positive cells. Staining cervical smears of HIV+ women is a simple procedure to get an indication of clinical outcome of the patient.
authors.affiliation: Leiden Cytology and Pathology Laboratory, Leiden, The Netherlands. m.e.boon@lcpl.nl
authors.forename: M E
authors.initials: ME
authors.lastname: Boon
authors.forename: G
authors.initials: G
authors.lastname: van der Veen
authors.forename: Y
authors.initials: Y
authors.lastname: Barlow
authors.forename: M S
authors.initials: MS
authors.lastname: Graudenz
authors.forename: L P
authors.initials: LP
authors.lastname: Kok
mesh_terms: D000328:Adult; D034961:Antigens, Nuclear; D015415:Biomarkers; D001938:Brazil / Q000453:epidemiology; D002455:Cell Division; D002584:Cervix Uteri / Q000737:chemistry / Q000473:pathology; D005260:Female; D005500:Follow-Up Studies; D015658:HIV Infections / Q000150:complications*; D006801:Humans; D019394:Ki-67 Antigen; D008297:Male; D009687:Nuclear Proteins / Q000032:analysis*; D065006:Papanicolaou Test; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification; D030361:Papillomavirus Infections / Q000821:virology; D010865:Pilot Projects; D014412:Tumor Virus Infections / Q000821:virology; D002583:Uterine Cervical Neoplasms / Q000150:complications / Q000453:epidemiology* / Q000473:pathology / Q000821:virology; D014626:Vaginal Smears; D016387:Women's Health; D002578:Uterine Cervical Dysplasia / Q000150:complications / Q000453:epidemiology* / Q000473:pathology / Q000821:virology
chemical_list: D034961:Antigens, Nuclear; D015415:Biomarkers; D019394:Ki-67 Antigen; D009687:Nuclear Proteins