title: Determination of trovafloxacin in human serum by time resolved terbium-sensitised luminescence.
abstract: A sensitive time-resolved luminescence method for the determination of trovafloxacin is described. The method is based on the time-resolved luminescence signal from the terbium(III)-trovafloxacin complex, in a micellar solution of sodium dodecyl sulfate (SDS), using a chemical deoxygenation agent (Na(2)SO(3)). The method allows the determination of 20-450 ng ml(-1) of trovafloxacin in 7.5 mM SDS solution containing 0.16 M acetic acid-sodium acetate buffer (pH 6.0) and 7.5 mM Na(2)SO(3) with lambda(exc)=270 nm and lambda(em)=546 nm. In these experimental conditions luminescence signal for trovafloxacin increases 20-fold with respect to native fluorescence of the compound in aqueous solution at pH 6.5. Terbium-sensitised luminescence was applied to trovafloxacin determination in human serum, spiked at levels found after drug administration at normal clinical doses. Recovery is 90+/-1% and day-to-day precision is 3.5%. The proposed method tolerates high concentrations of other co-administrated drugs and excipients.
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemistry, 41012 Seville, Spain.
authors.forename: J A
authors.initials: JA
authors.lastname: Ocaña
authors.forename: M
authors.initials: M
authors.lastname: Callejón
authors.forename: F J
authors.initials: FJ
authors.lastname: Barragán
mesh_terms: D000890:Anti-Infective Agents / Q000097:blood*; D005079:Excipients; D024841:Fluoroquinolones; D007202:Indicators and Reagents; D008163:Luminescent Measurements; D009287:Naphthyridines / Q000097:blood*; D012967:Sodium Dodecyl Sulfate; D013050:Spectrometry, Fluorescence; D013501:Surface-Active Agents; D013725:Terbium / Q000737:chemistry*
chemical_list: D000890:Anti-Infective Agents; D005079:Excipients; D024841:Fluoroquinolones; D007202:Indicators and Reagents; D009287:Naphthyridines; D013501:Surface-Active Agents; D013725:Terbium; D012967:Sodium Dodecyl Sulfate; C080163:trovafloxacin