Opioid neurotransmission in the post-ictal analgesia: involvement of mu(1)-opioid receptor.
Pentylenetetrazol (PTZ), a non-competitive antagonist that blocks GABA-mediated Cl(-) flux, was used in the present work to induce seizures in animals. The aim of this work is to study the neurochemical basis of the antinociception induced by convulsions elicited by peripheral administration of PTZ (64 mg/kg). The analgesia was measured by the tail-flick test, in eight rats per group. Convulsions were followed by significative increase in the tail-flick latencies (TFL), for at least 120 min of the post-ictal period. Peripheral administration of naltrexone (5 mg/kg, 10 mg/kg and 20 mg/kg) caused a significant decrease in the TFL in seizing animals, as compared to controls. These data were corroborated with peripheral administration of naloxonazine (10 mg/kg and 20 mg/kg), a mu(1)-opioid blocker, in the same doses used for non-specific antagonist. These results indicate that endogenous opioids may be involved in the post-ictal analgesia. The involvement of mu(1)-opioid receptor was also considered.
Coimbra
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NC
Laboratório de Neuroanatomia e Neuropsicobiologia, Departamento de Farmacologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, 14049-900, Avenida dos Bandeirantes, 3900, Ribeirão Preto (SP), Brazil. nccoimbr@fmrp.usp.br
Freitas
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RL
Savoldi
M
M
Castro-Souza
C
C
Segato
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EN
Kishi
R
R
Weltson
A
A
Resende
G C
GC
D000698:Analgesia; D000818:Animals; D003292:Convulsants; D004833:Epilepsy, Temporal Lobe / Q000139:chemically induced / Q000378:metabolism; D004830:Epilepsy, Tonic-Clonic / Q000139:chemically induced / Q000378:metabolism*; D009271:Naltrexone / Q000494:pharmacology; D009292:Narcotic Antagonists / Q000494:pharmacology; D018847:Opioid Peptides / Q000378:metabolism*; D017288:Pain Threshold / Q000502:physiology*; D010433:Pentylenetetrazole; D051381:Rats; D017208:Rats, Wistar; D017450:Receptors, Opioid, mu / Q000037:antagonists & inhibitors / Q000378:metabolism*; D009435:Synaptic Transmission / Q000502:physiology*
D003292:Convulsants; D009292:Narcotic Antagonists; D018847:Opioid Peptides; D017450:Receptors, Opioid, mu; D009271:Naltrexone; D010433:Pentylenetetrazole