title: Orexin-A regulates body temperature in coordination with arousal status.
abstract: Orexins, hypothalamic neuropeptides, are involved in modulation of food intake and arousal status. To further examine their physiological roles in brain function, the effect of centrally administered orexin-A on body temperature was investigated in rats. Assessed by a telemetry sensor system implanted into the abdominal cavity, infusion of orexin-A into the third cerebroventricle (i3vt) increased body temperature in a dose-responsive manner. Expression of uncoupling protein 1 (UCP1) mRNA in brown adipose tissue (BAT), as a marker for peripheral thermogenesis, failed to increase after the infusion. Expression of UCP3 mRNA in skeletal muscle was up-regulated, whereas UCP2 in white adipose tissue was unchanged after the infusion. The resulting information indicates that orexin neurons regulate body temperature in coordination with arousal status independently of peripheral thermogenesis, which is regulated by BAT UCP1.
authors.affiliation: Department of Internal Medicine I, School of Medicine, Oita Medical University, Japan.
authors.forename: G
authors.initials: G
authors.lastname: Yoshimichi
authors.forename: H
authors.initials: H
authors.lastname: Yoshimatsu
authors.forename: T
authors.initials: T
authors.lastname: Masaki
authors.forename: T
authors.initials: T
authors.lastname: Sakata
mesh_terms: D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism; D002001:Adipose Tissue, Brown / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D001143:Arousal / Q000187:drug effects* / Q000502:physiology; D001483:Base Sequence; D001786:Blood Glucose / Q000378:metabolism; D001833:Body Temperature Regulation / Q000187:drug effects* / Q000502:physiology; D002352:Carrier Proteins / Q000008:administration & dosage / Q000235:genetics / Q000494:pharmacology* / Q000502:physiology; D002940:Circadian Rhythm / Q000187:drug effects / Q000502:physiology; D017931:DNA Primers / Q000235:genetics; D005230:Fatty Acids, Nonesterified / Q000097:blood; D007263:Infusions, Parenteral; D007328:Insulin / Q000097:blood; D047908:Intracellular Signaling Peptides and Proteins; D007473:Ion Channels; D008297:Male; D008565:Membrane Proteins / Q000235:genetics; D026901:Membrane Transport Proteins; D024101:Mitochondrial Proteins; D009043:Motor Activity / Q000187:drug effects; D018482:Muscle, Skeletal / Q000187:drug effects / Q000378:metabolism; D009479:Neuropeptides / Q000008:administration & dosage / Q000494:pharmacology* / Q000502:physiology; D000068797:Orexins; D011506:Proteins / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D000071256:Uncoupling Protein 1; D000071246:Uncoupling Protein 2; D000071247:Uncoupling Protein 3
chemical_list: D001786:Blood Glucose; D002352:Carrier Proteins; D017931:DNA Primers; D005230:Fatty Acids, Nonesterified; D007328:Insulin; D047908:Intracellular Signaling Peptides and Proteins; D007473:Ion Channels; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D024101:Mitochondrial Proteins; D009479:Neuropeptides; D000068797:Orexins; D011506:Proteins; D012333:RNA, Messenger; C000604373:Ucp1 protein, rat; C000604391:Ucp2 protein, rat; C000604394:Ucp3 protein, rat; D000071256:Uncoupling Protein 1; D000071246:Uncoupling Protein 2; D000071247:Uncoupling Protein 3