title: Alternative splicing of fibronectin transcripts in osteochondrogenic tumors.
abstract: BACKGROUND
  Fibronectin (FN) acts in many fundamental biological processes including cell adhesion, migration, proliferation and apoptosis. Although FN shows an ubiquitous expression pattern, alternative mRNA splicing modulates tissue-specific molecular heterogeneity at three regions: extra domain-B, extra domain-A and variable region.
  
  
  METHODS
  In the present study, we analyzed the FN mRNA splicing in osteochondrogenic tumors as well as in various normal tissues using the RT-PCR method.
  
  
  RESULTS
  Normal cartilage almost exclusively contained short mRNA lacking alternative exons in all regions. However, 14 osteochondrogenic tumors including osteochondroma, enchondroma, chondroblastoma, chondrosarcoma and osteosarcoma uniformly contained long mRNA with various patterns of alternative exons in the three regions.
  
  
  CONCLUSIONS
  These results indicated that the lack of regulation excluding the alternative exons may be associated with tumorigenesis in chondrocytes.
authors.affiliation: Department of Orthopaedic Surgery, Osaka Medical Center and Research Institute for Maternal and Child Health, 840 Murodocho, Izumi, Osaka 594-1101, Japan. ymatsui@sam.hi-ho.ne.jp
authors.forename: Y
authors.initials: Y
authors.lastname: Matsui
authors.forename: K
authors.initials: K
authors.lastname: Nakata
authors.forename: N
authors.initials: N
authors.lastname: Araki
authors.forename: K
authors.initials: K
authors.lastname: Ozono
authors.forename: Y
authors.initials: Y
authors.lastname: Fujita
authors.forename: N
authors.initials: N
authors.lastname: Tsumaki
authors.forename: H
authors.initials: H
authors.lastname: Kawabata
authors.forename: N
authors.initials: N
authors.lastname: Yasui
authors.forename: K
authors.initials: K
authors.lastname: Sekiguchi
authors.forename: H
authors.initials: H
authors.lastname: Yoshikawa
mesh_terms: D017398:Alternative Splicing; D001859:Bone Neoplasms / Q000235:genetics / Q000473:pathology; D002804:Chondroblastoma / Q000235:genetics / Q000473:pathology; D002812:Chondroma / Q000235:genetics / Q000473:pathology; D002813:Chondrosarcoma / Q000235:genetics / Q000473:pathology; D005353:Fibronectins / Q000235:genetics*; D006801:Humans; D009372:Neoplasms, Connective Tissue / Q000235:genetics* / Q000473:pathology; D015831:Osteochondroma / Q000235:genetics / Q000473:pathology; D012516:Osteosarcoma / Q000235:genetics / Q000473:pathology; D012333:RNA, Messenger
chemical_list: D005353:Fibronectins; D012333:RNA, Messenger