Regions of FtsZ important for self-interaction in Staphylococcus aureus.
Since FtsZ is structurally similar to eukaryotic tubulin, selective regions of S. aureus FtsZ have been swapped with equivalent regions from tubulin. These mutant FtsZ proteins were analyzed for their interaction with both wildtype FtsZ and FtsA using the yeast two-hybrid assay. Specific regions were identified which abrogated FtsZ-FtsZ binding but unaffected the FtsA-FtsZ interaction. This work will further our understanding of the regions of FtsZ critical for its physiological role.
Yan
K
K
Department of Microbiology, UP1345, GlaxoSmithKline, 1250 South Collegeville Road, Collegeville, Pennsylvania 19426, USA. Kang_Yan-1@sbphrd.com
Sossong
T M
TM
Payne
D J
DJ
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001665:Binding Sites / Q000502:physiology; D003001:Cloning, Molecular; D003598:Cytoskeletal Proteins; D017930:Genes, Reporter; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D017354:Point Mutation; D011485:Protein Binding / Q000502:physiology; D017434:Protein Structure, Tertiary / Q000502:physiology; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013211:Staphylococcus aureus; D013329:Structure-Activity Relationship; D014404:Tubulin / Q000235:genetics / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
D001426:Bacterial Proteins; D003598:Cytoskeletal Proteins; C071525:FtsZ protein, Bacteria; D011993:Recombinant Fusion Proteins; D014404:Tubulin