Mapping and promoter sequencing of HNF-1beta gene in diabetes-prone and -resistant mice.
By using a novel single nucleotide polymorphism (SNP) in the coding sequence, the chromosomal location of Tcf2, encoding hepatic nuclear factor (HNF)-1beta, was determined in F2 intercrosses between Nagoya-Shibata-Yasuda (NSY) mice, an animal model of type 2 diabetes, and control C3H/He mice. The promoter region of Tcf2 gene was sequenced in NSY, non-obese diabetic (NOD) and control C3H/He mice. Tcf2 was mapped between genetic markers D11MIT320 and D11MIT195 with the following distances: D11MIT320-(7.3 cM)-Tcf2-(0.5 cM)-D11MIT195. A variant with insertion of C between -205 and -204 in the promoter region of Tcf2 was identified in NSY mice, but not NOD and C3H/He mice.
Ueda
H
H
Department of Geriatric Medicine, Osaka University Medical School, 2-2 Yamadaoka, Suita, 565-0871, Osaka, Japan.
Ikegami
H
H
Kawaguchi
Y
Y
Fujisawa
T
T
Nojima
K
K
Babaya
N
N
Yamada
K
K
Shibata
M
M
Yamato
E
E
Ogihara
T
T
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D004268:DNA-Binding Proteins / Q000235:genetics*; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics*; D004195:Disease Models, Animal; D005819:Genetic Markers; D020022:Genetic Predisposition to Disease; D051539:Hepatocyte Nuclear Factor 1-beta; D051379:Mice; D008809:Mice, Inbred C3H; D016688:Mice, Inbred NOD; D008815:Mice, Inbred Strains; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D012016:Reference Values; D014157:Transcription Factors / Q000235:genetics*
D004268:DNA-Binding Proteins; D005819:Genetic Markers; C496496:Hnf1b protein, mouse; D014157:Transcription Factors; D051539:Hepatocyte Nuclear Factor 1-beta