title: Sustained parasite burden in the spleen of Leishmania infantum-infected BALB/c mice is accompanied by expression of MCP-1 transcripts and lack of protection against challenge.
abstract: We analyzed differential responses of spleen and liver, major organ targets for viscerotropic Leishmania species, to experimental infection and examined if resistance to challenge was organ-specific. In liver, parasites were spontaneously cleared and iNOS trancripts expression paralleled that of amastigote load. In the spleen, amastigote multiplication was only partly controlled, and iNOS transcripts expression was transient. Total numbers of spleen cells, B cells, and T cells were decreased, while F4/80(+) and Mac1(+) cells were conserved. Expression of splenic MCP-1 transcripts remained constant, indicating its possible contribution to immigration of Leishmania host cells and to sustained parasite load. Spleen cells produced both, Th1- and Th2-type cytokines and Th2-type response was dominant, compatible with the sustained MCP-1 expression. Challenge experiments showed that in contrast to the liver, where initial infection conferred a progressively established immunity, in the spleen there was no induced protection against reinfection. Organ-specific resistance against challenge could be important for designing antileishmanial vaccines.
authors.affiliation: Groupe de Recherche en Immunopathologie de la Leishmaniose (EA 2675), Laboratoire de Parasitologie, Faculté de Médecine, avenue de Valombrose, 06107 Nice Cedex 2, France.
authors.forename: D
authors.initials: D
authors.lastname: Rousseau
authors.forename: S
authors.initials: S
authors.lastname: Demartino
authors.forename: F
authors.initials: F
authors.lastname: Anjuère
authors.forename: B
authors.initials: B
authors.lastname: Ferrua
authors.forename: K
authors.initials: K
authors.lastname: Fragaki
authors.forename: Y
authors.initials: Y
authors.lastname: Le Fichoux
authors.forename: J
authors.initials: J
authors.lastname: Kubar
mesh_terms: D000818:Animals; D001483:Base Sequence; D018932:Chemokine CCL2 / Q000235:genetics*; D006224:Cricetinae; D017931:DNA Primers; D006801:Humans; D018314:Leishmania infantum / Q000302:isolation & purification*; D007898:Leishmaniasis, Visceral / Q000276:immunology / Q000469:parasitology; D008099:Liver / Q000201:enzymology / Q000276:immunology / Q000469:parasitology; D008647:Mesocricetus; D051379:Mice; D008807:Mice, Inbred BALB C; D019001:Nitric Oxide Synthase / Q000235:genetics; D052247:Nitric Oxide Synthase Type II; D012333:RNA, Messenger / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013154:Spleen / Q000201:enzymology / Q000276:immunology / Q000469:parasitology*; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology
chemical_list: D018932:Chemokine CCL2; D017931:DNA Primers; D012333:RNA, Messenger; C496319:NOS2 protein, human; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse