title: Lysophosphatidic acid- and Gbeta-dependent activation of Dictyostelium MAP kinase ERK2.
abstract: Exogenous lysophosphatidic acid (LPA) has been shown to evoke a chemotactic response in aggregative cells of the social amoeba Dictyostelium discoideum. In this paper, we demonstrate that extracellular LPA is also able to induce activation of mitogen-activated protein (MAP) kinase DdERK2 (extracellular signal regulated kinase 2) in these cells. This activation is independent of cyclic AMP receptors, yet fully dependent on the single Gbeta subunit, hinting to the presence of functional heptahelical LPA receptors in a primitive eukaryote. We did not observe LPA-dependent cyclic GMP accumulation, which suggests that the pathways for LPA-induced and "classical" chemotaxis of D. discoideum cells are substantially different.
authors.affiliation: Institute of Molecular Plant Sciences, Leiden University, Leiden, 2300 RA, The Netherlands.
authors.forename: P W
authors.initials: PW
authors.lastname: Schenk
authors.forename: S J
authors.initials: SJ
authors.lastname: Epskamp
authors.forename: M L
authors.initials: ML
authors.lastname: Knetsch
authors.forename: V
authors.initials: V
authors.lastname: Harten
authors.forename: E L
authors.initials: EL
authors.lastname: Lagendijk
authors.forename: B
authors.initials: B
authors.lastname: van Duijn
authors.forename: B E
authors.initials: BE
authors.lastname: Snaar-Jagalska
mesh_terms: D000818:Animals; D002633:Chemotaxis / Q000187:drug effects; D000242:Cyclic AMP / Q000378:metabolism; D006152:Cyclic GMP / Q000378:metabolism; D004023:Dictyostelium / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D004789:Enzyme Activation / Q000187:drug effects; D020962:Heterotrimeric GTP-Binding Proteins / Q000378:metabolism*; D008246:Lysophospholipids / Q000378:metabolism / Q000494:pharmacology*; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000187:drug effects / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled; D049368:Receptors, Lysophosphatidic Acid; D015398:Signal Transduction
chemical_list: D008246:Lysophospholipids; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; D049368:Receptors, Lysophosphatidic Acid; D000242:Cyclic AMP; D019950:Mitogen-Activated Protein Kinase 1; D020962:Heterotrimeric GTP-Binding Proteins; D006152:Cyclic GMP