Regenerative capacity of human satellite cells: the mitotic clock in cell transplantation.
In this communication, we will review the problems caused by cell-mediated gene therapy, taking skeletal muscle as a physiological model. In particular we have utilised vectors transferring telomerase under the control of retroviral promoters into human satellite cells. The set of results presented here has several implications regarding gene therapy trials. Nevertheless, more experiments will be required to fully validate this cellular model and to use telomerase to safely extend the lifespan of putative gene therapy vectors.
Di Donna
S
S
CNRS UMR 7000, Cytoskeleton and Development, 105 bd de l'Hôpital, 75634 Paris, France.
Renault
V
V
Forestier
C
C
Piron-Hamelin
G
G
Thiesson
D
D
Cooper
R N
RN
Ponsot
E
E
Decary
S
S
Amouri
R
R
Hentati
F
F
Butler-Browne
G S
GS
Mouly
V
V
D000818:Animals; D001683:Biological Clocks / Q000235:genetics; D016922:Cellular Senescence / Q000502:physiology; D015316:Genetic Therapy; D005822:Genetic Vectors / Q000502:physiology; D006801:Humans; D008938:Mitosis / Q000502:physiology; D018485:Muscle Fibers, Skeletal / Q000166:cytology / Q000378:metabolism / Q000637:transplantation*; D009136:Muscular Dystrophies / Q000235:genetics / Q000503:physiopathology / Q000628:therapy; D016615:Telomere / Q000235:genetics; D016378:Tissue Transplantation / Q000379:methods* / Q000639:trends