Production of beta-mannanase and beta-mannosidase from Aspergillus awamori K4 and their properties.
beta-Mannanase and beta-mannosidase from Aspergillus awamori K4 was produced by solid culture with coffee waste and wheat bran. The optimum composition for enzyme production was 40% coffee waste-60% wheat bran. Two enzymes were partially purified. Optimum pH was about 5 for both enzymes, and optimum temperature was around 80 degrees C for beta-mannanase and 60-70 degrees C for beta-mannosidase. These enzymes produced some oligosaccharides from glucomannan and galactomannan by their hydrolyzing and transferring activities. beta-Mannanase hydrolyzed konjak and locust bean gum 39.1% and 15.8%, respectively. Oligosaccharides of various molecular size were released from glucomannan of konjak, but on the addition of cellulase, mannobiose was released selectively. In locust bean gum, tetra-, tri-, and disaccharides (mannobiose) were mainly released by K4 beta-mannanase. Tetra- and trisaccharides were heterooligosaccharides consisting of galactose and mannose residues. K4 beta-mannosidase had a transglycosylation action, transferring mannose residue to alcohols and sugars like fructose.
Kurakake
M
M
Department of Applied Biological Science, Fukuyama University, Sanzou, Gakuenchou 1 banchi, Fukuyama, Hiroshima 729-0292, Japan. kurakake@fubac.fukuyama-u.ac.jp
Komaki
T
T
D001230:Aspergillus / Q000201:enzymology* / Q000254:growth & development; D001673:Biodegradation, Environmental; D003069:Coffee; D004043:Dietary Fiber; D004790:Enzyme Induction; D005685:Galactans / Q000378:metabolism; D006031:Glycosylation; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008351:Mannans / Q000378:metabolism; D008361:Mannosidases / Q000096:biosynthesis* / Q000302:isolation & purification*; D013379:Substrate Specificity; D044902:beta-Mannosidase
D003069:Coffee; D004043:Dietary Fiber; D005685:Galactans; D008351:Mannans; D008361:Mannosidases; D044902:beta-Mannosidase