Enhancement of Taxol production and excretion in Taxus chinensis cell culture by fungal elicitation and medium renewal.
An endophytic fungus, Aspergillus niger, isolated from the inner bark of a Taxus chinensis tree, was used as an elicitor to stimulate the Taxol (paclitaxel) production in a Taxus chinensis cell suspension culture. Different elicitor doses and elicitation times were tested in a batch culture; and the highest volumetric Taxol yield was achieved when 40 mg of the fungal elicitor (carbohydrate equivalent) l(-1) was added to the culture during the late exponential-growth phase. The elicitation resulted in a more than two-fold increase in the Taxol yield and about a six-fold increase in total secretion. The Taxol yield was further improved substantially by applying medium renewal and re-elicitation to the culture. In particular, with repeated medium renewal (in a way similar to medium perfusion) and a second elicitation of the culture, the volumetric Taxol yield was increased to 67.1+/-7.5 mg l(-1), which was about seven times the amount obtained in the non-elicited batch culture. The Taxol productivity of the perfusion-like culture with repeated fungal elicitation was 1.5 mg l(-1) day(-1), which was about 40% higher than that of the elicitor-treated batch culture and three times the productivity of the non-elicited batch culture.
Wang
C
C
Institute of Biotechnology, Huazhong University of Science and Technology, Wuhan, PR China.
Wu
J
J
Mei
X
X
D000972:Antineoplastic Agents, Phytogenic / Q000096:biosynthesis*; D002455:Cell Division; D002478:Cells, Cultured; D005658:Fungi / Q000302:isolation & purification / Q000502:physiology*; D017239:Paclitaxel / Q000096:biosynthesis*; D014197:Trees / Q000166:cytology / Q000378:metabolism* / Q000382:microbiology
D000972:Antineoplastic Agents, Phytogenic; D017239:Paclitaxel