title: Recombinant equine arteritis virus as an expression vector.
abstract: Equine arteritis virus (EAV) is the prototypic member of the family Arteriviridae, which together with the Corona- and Toroviridae constitutes the order Nidovirales. A common trait of these positive-stranded RNA viruses is the 3'-coterminal nested set of six to eight leader-containing subgenomic mRNAs which are generated by a discontinuous transcription mechanism and from which the viral open reading frames downstream of the polymerase gene are expressed. In this study, we investigated whether the unique gene expression strategy of the Nidovirales could be utilized to convert them into viral expression vectors by introduction of an additional transcription unit into the EAV genome directing the synthesis of an extra subgenomic mRNA. To this end, an expression cassette consisting of the gene for a green fluorescent protein (GFP) flanked at its 3' end by EAV-specific transcription-regulating sequences was constructed. This genetic module was inserted into the recently obtained mutant infectious EAV cDNA clone pBRNX1.38-5/6 (A. A. F. de Vries, et al., 2000, Virology 270, 84-97) between the genes for the M and the G(L) proteins. Confocal fluorescence microscopy of BHK-21 cells electroporated with capped RNA transcripts derived from the resulting plasmid (pBRNX1.38-5/6-GFP) demonstrated that the GFP gene was expressed in the transfected cells, while the gradual spread of the infection through the cell monolayer showed that the recombinant virus was replication competent. The development of the cytopathic effect was, however, much slower than in cells that had received equivalent amounts of pBRNX1.38-5/6 RNA, indicating that the vector virus had a clear growth disadvantage compared to its direct precursor. Immunoprecipitation analyses of proteins from metabolically labeled BHK-21 cells infected with supernatant of the transfected cultures confirmed that the recombinant virus vector was viable and expressed viral genes as well as the GFP gene. Reverse transcription-PCR of the viral mRNAs extracted from cells infected with the vector virus revealed that it directed the synthesis of nine instead of eight different EAV RNAs. These findings were corroborated by hybridization analyses. Mapping of the leader-to-body junctions of the ninth mRNA indicated that the 3' part of the GFP gene contains cryptic transcription signals which gave rise to at least five different RNA species ranging in size from 1277 to 1439 nt [without oligo(A) tract]. Furthermore, translation of the unintended mRNA resulted in the production of an extended version of the EAV M protein. Serial passage of the recombinant virus vector led to its gradual replacement by viral mutants carrying deletions in the GFP gene. The reduction in viral fitness associated with the insertion of the expression cassette into the EAV genome apparently caused genetic instability of the recombinant virus.
authors.affiliation: Virology Division, Department of Infectious Diseases and Immunology, Veterinary Faculty, Utrecht University, Yalelaan 1, Utrecht, 3584 CL, The Netherlands.
authors.forename: A A
authors.initials: AA
authors.lastname: de Vries
authors.forename: A L
authors.initials: AL
authors.lastname: Glaser
authors.forename: M J
authors.initials: MJ
authors.lastname: Raamsman
authors.forename: P J
authors.initials: PJ
authors.lastname: Rottier
mesh_terms: D000595:Amino Acid Sequence; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D003593:Cytoplasm / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D007240:Equartevirus / Q000235:genetics* / Q000472:pathogenicity; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins; D015151:Immunoblotting; D008164:Luminescent Proteins / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics; D018613:Microscopy, Confocal; D008969:Molecular Sequence Data; D012313:RNA / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D012367:RNA, Viral / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic; D014170:Transformation, Genetic; D015678:Viral Structural Proteins / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics; D014779:Virus Replication
chemical_list: D018076:DNA, Complementary; D008164:Luminescent Proteins; D012333:RNA, Messenger; D012367:RNA, Viral; D015678:Viral Structural Proteins; D049452:Green Fluorescent Proteins; D012313:RNA