Differences in splenic B-lymphocyte ganglioside expression and accessibility in normal and endotoxin-hyporesponsive mice.
Endotoxin-responsive (C3H/HeN) and -hyporesponsive (C3H/HeJ) murine B lymphocytes purified by adherence to anti-immunoglobulin ("antibody panning") possess identical gangliosides but different ganglioside surface accessibilities. We investigated the distribution and surface accessibility of gangliosides of B lymphocytes purified by adherence to plastic ("plastic panning") or by subtraction of non-B-lymphocyte components. As with antibody panning, there were no entirely new or absent gangliosides in plastic-panned or subtraction-purified B lymphocytes of each strain. However, striking changes in relative expression of five gangliosides were detected with each purification protocol. Moreover, five gangliosides of antibody-panned and plastic-panned B lymphocytes but only two gangliosides of subtraction-purified B lymphocytes were inaccessible to surface labeling. Unlike the situation for antibody-panned B lymphocytes, no interstrain (HeN vs. HeJ) surface accessibility differences existed in gangliosides of plastic-panned or subtraction-purified cells. Exposure of subtraction-purified B lymphocytes to anti-immunoglobulin failed to elicit changes in ganglioside expression. Murine B lymphocytes have distinct protocol-dependent differences in glycolipid phenotype which likely denote individual subpopulations.
Berenson
C S
CS
Infectious Diseases Section, Department of Veterans Affairs Western New York Healthcare System and State University of New York at Buffalo, Buffalo, New York, USA. berenson@acsu.buffalo.edu
Rasp
R H
RH
Gau
J T
JT
Ryan
J L
JL
Yohe
H C
HC
D000818:Animals; D016175:B-Lymphocyte Subsets / Q000378:metabolism*; D002448:Cell Adhesion; D002469:Cell Separation / Q000379:methods*; D002855:Chromatography, Thin Layer; D019446:Endotoxemia / Q000235:genetics / Q000276:immunology*; D004731:Endotoxins / Q000633:toxicity*; D005732:Gangliosides / Q000378:metabolism*; D020022:Genetic Predisposition to Disease; D007113:Immunity, Innate; D051379:Mice; D008809:Mice, Inbred C3H; D010969:Plastics; D011947:Receptors, Antigen, B-Cell / Q000032:analysis; D012794:Sialic Acids / Q000032:analysis; D013154:Spleen / Q000378:metabolism*
D004731:Endotoxins; D005732:Gangliosides; D010969:Plastics; D011947:Receptors, Antigen, B-Cell; D012794:Sialic Acids