Expression of Fushi tarazu factor 1 homolog and Pit-1 genes in the pituitaries of pre-spawning chum and sockeye salmon.
Fushi tarazu factor-1 (FTZ-F1) and Pit-1 are major pituitary transcription factors, controlling expression of genes coding for gonadotropin (GTH) subunits and growth hormone/prolactin/somatolactin family hormone, respectively. As a first step to investigate physiological factors regulating gene expression of these transcription factors, we determined their mRNA levels in the pituitaries of chum salmon (Oncorhynchus keta) at different stages of sexual maturation. FTZ-F1 gene expression was increased in males at the stage before spermiation, where the levels of GTH alpha and IIbeta subunit mRNAs were elevated. Pit-1 mRNA showed maximum levels at the final stage of sexual maturation in both sexes, when expression of somatolactin gene peaked. To clarify whether gonadotropin-releasing hormone (GnRH) is involved in these increases in FTZ-F1 and Pit-1 gene expression, we examined effects of GnRH analog (GnRHa) administration on their gene expression in maturing sockeye salmon (Oncorhynchus nerka). GnRHa stimulated Pit-1 gene expression in females only, but failed to stimulate FTZ-F1 gene expression in both sexes. The up-regulated expression of FTZ-F1 and Pit-1 genes at the pre-spawning stages suggest that the two transcription factors have roles in sexual maturation of salmonids. Physiological factors regulating gene expression of FTZ-F1 and Pit-1 are discussed in this review.
Higa
M
M
Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo, 060-0810, Hokkaido, Japan. higa@sci.hokudai.ac.jp
Ando
H
H
Urano
A
A
D000818:Animals; D004268:DNA-Binding Proteins / Q000235:genetics*; D051717:Fushi Tarazu Transcription Factors; D018398:Homeodomain Proteins; D017685:Oncorhynchus keta / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D010902:Pituitary Gland / Q000378:metabolism*; D018160:Receptors, Cytoplasmic and Nuclear; D012474:Salmon / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D054339:Steroidogenic Factor 1; D050817:Transcription Factor Pit-1; D014157:Transcription Factors / Q000235:genetics*
D004268:DNA-Binding Proteins; D051717:Fushi Tarazu Transcription Factors; D018398:Homeodomain Proteins; D018160:Receptors, Cytoplasmic and Nuclear; D054339:Steroidogenic Factor 1; D050817:Transcription Factor Pit-1; D014157:Transcription Factors