A novel filamentous Bacillus sp., strain NAF001, forming endospores and budding cells.
A novel filamentous bacterium, strain NAF001, was isolated from suspended water of a domestic wastewater treatment tank. It formed an extremely long filamentous trichome and produced endospores. It formed spore-like resting cells (SLRCs) which were heat-resistant. SLRCs grew by budding to form short filaments resembling the gonidia of filamentous bacteria such as LEUCOTHRIX: This is the first report of a Bacillus species that exhibits budding growth. The filamentous form was neither restricted to any particular growth stage nor dependent on cultural conditions. Phylogenetic analysis of the 16S rRNA gene revealed that this isolate was a member of the genus Bacillus, with no close relatives at the species level (sequence similarity <95.3%). Strain NAF001 thus probably belongs to a new and novel species of Bacillus.
Ajithkumar
Vasudevan P
VP
Laboratory of Ecological and Toxicological Chemistry, Faculty of Agriculture, Shinshu University (United Graduate School of Gifu University), 8304 Minamiminowa, Kamiina, Nagano 399-4598, Japan1.
Ajithkumar
Bindu
B
Laboratory of Ecological and Toxicological Chemistry, Faculty of Agriculture, Shinshu University (United Graduate School of Gifu University), 8304 Minamiminowa, Kamiina, Nagano 399-4598, Japan1.
Mori
Koji
K
Laboratory of Microbial Engineering and Technology, Faculty of Agriculture, Gifu University (United Graduate School of Gifu University), 1-1, Yanagido, Gifu 501-1193, Japan2.
Takamizawa
Kazuhiro
K
Laboratory of Microbial Engineering and Technology, Faculty of Agriculture, Gifu University (United Graduate School of Gifu University), 1-1, Yanagido, Gifu 501-1193, Japan2.
Iriye
Ryozo
R
Laboratory of Ecological and Toxicological Chemistry, Faculty of Agriculture, Shinshu University (United Graduate School of Gifu University), 8304 Minamiminowa, Kamiina, Nagano 399-4598, Japan1.
Tabata
Shinichiro
S
Laboratory of Ecological and Toxicological Chemistry, Faculty of Agriculture, Shinshu University (United Graduate School of Gifu University), 8304 Minamiminowa, Kamiina, Nagano 399-4598, Japan1.
D001407:Bacillus / Q000302:isolation & purification / Q000502:physiology* / Q000648:ultrastructure; D001482:Base Composition; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D006358:Hot Temperature; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D012689:Sequence Homology, Nucleic Acid; D012722:Sewage / Q000382:microbiology; D013171:Spores, Bacterial / Q000254:growth & development / Q000502:physiology / Q000648:ultrastructure
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012722:Sewage