title: Hemoglobin adducts as biomarkers of 1,3-butadiene in occupationally low exposed Italian workers and a few diesel-exposed miners.
abstract: Hemoglobin adducts were determined as biomarkers of 1,3-butadiene (BD) in 30 workers and 10 controls from an Italian BD plant and in 14 diesel-exposed miners. N-(2,3,4-trihydroxybutyl)valine (THBVal), an N-terminal valine globin adduct of reactive butadiene metabolites, was analyzed by gas chromatography/high resolution mass spectrometry after a modified Edman degradation and further acetylation. The BD exposure for the plant workers was 31 microg/m(3) (personal sampling). Whereas there was no detectable difference in hemoglobin adduct levels (range 17.7-61.4 pmol/g globin) between the total group of exposed and controls, slight but significant differences could be found between two subgroups of workers from different production units as well as one subgroup and controls (P<0.05), between smoking (n=13) and non-smoking exposed workers (n=17; P=0.066) as well as between smoking exposed workers and controls (P=0.055). Adduct levels of the miners (all non-smokers) were in the same range as those of the Italian BD-workers and controls. The internal exposure and strain measured by THBVal levels resulting from a very low occupational BD exposure was in the range of the contribution of moderate smoking.
authors.affiliation: Department of Toxicology, University of Würzburg, Versbacher Strasse 9, D-97078 Würzburg, Germany.
authors.forename: P
authors.initials: P
authors.lastname: Begemann
authors.forename: P B
authors.initials: PB
authors.lastname: Upton
authors.forename: A
authors.initials: A
authors.lastname: Ranasinghe
authors.forename: J A
authors.initials: JA
authors.lastname: Swenberg
authors.forename: L
authors.initials: L
authors.lastname: Soleo
authors.forename: L
authors.initials: L
authors.lastname: Vimercati
authors.forename: A
authors.initials: A
authors.lastname: Gelormini
authors.forename: S
authors.initials: S
authors.lastname: Fustinoni
authors.forename: I
authors.initials: I
authors.lastname: Zwirner-Baier
authors.forename: H G
authors.initials: HG
authors.lastname: Neumann
mesh_terms: D015415:Biomarkers / Q000097:blood; D002070:Butadienes / Q000032:analysis / Q000633:toxicity*; D005742:Gasoline / Q000633:toxicity; D006454:Hemoglobins / Q000032:analysis / Q000737:chemistry* / Q000187:drug effects*; D006801:Humans; D007558:Italy; D008906:Mining; D016273:Occupational Exposure
chemical_list: D015415:Biomarkers; D002070:Butadienes; D005742:Gasoline; D006454:Hemoglobins; C031763:1,3-butadiene