Pretreatment of guinea-pigs with iron or desferal influences the course of Yersinia enterocolitica infections.
The effects of iron excess and desferrioxamine in pretreated guinea-pigs on the immune response (production of Yops) and on the histological changes in infections with Yersinia enterocolitica 0:3 and Y. enterocolitica 0:8 were investigated. The prior overload of the guinea pigs with Dextrofer or treatment with Desferal increased the pathogenic activity of Y. entercolitica 0:3 and led to a generalized infection. Immunoblot analysis showed that in conditions of iron overload the expression of outer membrane proteins (Yops) of Y. enterocolitica 0:8 was blocked. This was accompanied by weak changes in the tissues. The iron limited conditions stimulated production of a low molecular weight protein (17 kDa) on day 6 and easier proliferation of the bacterium. This in vivo study intends to show that in Y. enterocolitica infections a leading role is played not only by iron itself but also by the bacterial strain.
Nikolova
S
S
Institute of Microbiology, Department of Pathogenic Bacteria, Bulgarian Academy of Sciences, Sofia.
Wesselinova
D
D
Vesselinova
A
A
D000284:Administration, Oral; D000818:Animals; D000942:Antigens, Bacterial / Q000097:blood; D002614:Chelating Agents / Q000008:administration & dosage*; D003676:Deferoxamine / Q000008:administration & dosage*; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D005260:Female; D006168:Guinea Pigs; D015151:Immunoblotting / Q000662:veterinary; D007279:Injections, Subcutaneous / Q000662:veterinary; D007505:Iron-Dextran Complex / Q000008:administration & dosage*; D008297:Male; D011292:Premedication / Q000662:veterinary; D015009:Yersinia Infections / Q000276:immunology*; D015008:Yersinia enterocolitica / Q000145:classification* / Q000276:immunology / Q000472:pathogenicity*
D000942:Antigens, Bacterial; D002614:Chelating Agents; D007505:Iron-Dextran Complex; D003676:Deferoxamine