Structural investigations on the cell surface of Erysipelothrix rhusiopathiae.
The cell wall of Erysipelothrix rhusiopathiae was investigated. The corrected structure of the murein, which was published earlier, is reported here. The murein belongs to the B1delta type. It has L-alanine in position three of the peptide subunit and is crosslinked via a glycine-L-lysine-L-lysine interpeptide bridge. As expected by the complex serological situation in E. rhusiopathiae the carbohydrate moiety of the cell wall proofed to be very intricate. As accessory polymers polysaccharide(s) and teichoic acid(s) were identified. The teichoic acid(s) possess two novel polyols of which one could not be identified, the other one behaved like 6-O-methylgalactitol in gas liquid chromatography. Glucose and N-acetylgalactosamine were determined as substituents. The polysaccharide(s) is composed of mainly N-acetylfucosamine and smaller amounts of galactose, N-acetylglucosamine, and a further unidentified sugar appearing later than hexaacetylsorbitol in GLC.
Schubert
K
K
Institut für Genetik und Mikrobiologie der Universität München, Munich, Germany. K.Schubert@lrz.uni-muenchen.de
Fiedler
F
F
D000596:Amino Acids / Q000032:analysis; D000606:Amino Sugars / Q000032:analysis; D002473:Cell Wall / Q000737:chemistry*; D004888:Erysipelothrix / Q000737:chemistry*; D010457:Peptidoglycan / Q000737:chemistry*; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D013682:Teichoic Acids / Q000737:chemistry*
D000596:Amino Acids; D000606:Amino Sugars; D010457:Peptidoglycan; D011135:Polysaccharides, Bacterial; D013682:Teichoic Acids