A novel system for in vitro maturation of human oocytes.
OBJECTIVE
To compare in vitro maturation of cumulus-free oocytes in glucose-free medium (P1) and standard medium (TC199).


METHODS
Prospective, cohort study.


METHODS
Assisted reproductive technology program.


METHODS
One hundred eight patients undergoing ICSI.


METHODS
Germinal vesicle-stage or metaphase I--stage oocytes were allocated to culture with P1 or TC199. Metaphase II oocytes were fixed for immunofluorescence analysis or fluorescence in situ hybridization at 24 or 48 hours (or both). Media were compared by performing conditional logistic regression analysis that controlled for egg-specific factors.


METHODS
Proportion of mature oocytes and appearance of normal spindle-chromosome cytoarchitecture.


RESULTS
At 24 hours, more P1 oocytes than TC199 oocytes reached metaphase II (59.7% vs. 44.9%). At 48 hours, 71.7% of P1 oocytes and 61.0% of TC199 oocytes reached metaphase II, but this difference was not significant. Metaphase II oocytes in P1 were 34.3% more likely than those in TC199 to have a bipolar spindle with aligned chromosomes. Compared with oocytes at the germinal vesicle stage at 0 hour, those at metaphase I at 0 hour were more likely to progress to metaphase II (72.6% vs. 46.1% at 24 hours; 84.1% vs. 60.6% at 48 hours).


CONCLUSIONS
P1 is superior to TC199 for in vitro maturation of granulosa-free human oocytes.
Cekleniak
N A
NA
Department of Obstetrics and Gynecology and Reproductive Biology, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts, USA. nataliec@sbivf.com
Combelles
C M
CM
Ganz
D A
DA
Fung
J
J
Albertini
D F
DF
Racowsky
C
C
D000328:Adult; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000187:drug effects / Q000502:physiology; D002875:Chromosomes / Q000648:ultrastructure; D015331:Cohort Studies; D003470:Culture Media / Q000494:pharmacology; D005260:Female; D006801:Humans; D008677:Metaphase; D009865:Oocytes / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D011446:Prospective Studies; D008941:Spindle Apparatus / Q000648:ultrastructure; D013997:Time Factors
D003470:Culture Media