High-performance liquid chromatographic determination of methyl anthranilate, hydroxymethylfurfural and related compounds in honey.
A high-performance liquid chromatographic method for determining 5-hydroxymethyl-2-furaldehyde (hydroxymethylfurfural), 2-furaldehyde (furfural), furan-2-carboxylic acid (2-furoic acid), furan-3-carboxylic acid (3-furoic acid), furan-3-carboxaldehyde (3-furaldehyde) and 2-aminobenzoic acid methyl ester (methyl anthranilate) in honey and honeydew samples is described. To prevent matrix interference and to isolate the compounds, a clean-up step which implies a solid-phase extraction on polymeric cartridges and an elution with 0.5 ml methanol is recommended. The compounds are separated on a reversed-phase column with a gradient of (A) 1% aqueous acetic acid-acetonitrile (97:3, v/v) and (B) acetonitrile-water (50:50, v/v), with UV detection at 250 nm. The method is applied to the analysis of samples from different botanical origin.
Nozal
M J
MJ
Analytical Chemistry Department, Faculty of Sciences, University of Valladolid, Spain. mjdnozal@qa.uva.es
Bernal
J L
JL
Toribio
L
L
Jiménez
J J
JJ
Martín
M T
MT
D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005662:Furaldehyde / Q000031:analogs & derivatives* / Q000032:analysis*; D006722:Honey / Q000032:analysis*; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D062367:ortho-Aminobenzoates / Q000032:analysis*
D062367:ortho-Aminobenzoates; C008046:5-hydroxymethylfurfural; C038892:methyl anthranilate; D005662:Furaldehyde