A simple method for constructing microinjectrodes for reversible inactivation in behaving monkeys.
A method for constructing a simple, durable injection-microelectrode (injectrode) is described. The injectrode can record neuronal activity, stimulate neuronal tissues, or inject substances locally through its tip. The injectrode is lightweight and is easy to construct from commercially available parts, and it can be used repeatedly for multiple recordings and injections. Since dura penetration can damage fragile electrode tips, a reliable method to pass the injectrode through an intact dura matter is described.
Chen
L L
LL
Division of Neuroscience, Baylor College of Medicine, Houston, TX 77030, USA. lchen@cns.neusc.bcm.tmc.edu.
Goffart
L
L
Sparks
D L
DL
D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects / Q000502:physiology; D001921:Brain / Q000187:drug effects / Q000502:physiology / Q000601:surgery; D016503:Drug Delivery Systems / Q000295:instrumentation* / Q000379:methods; D004388:Dura Mater / Q000601:surgery; D004594:Electrophysiology / Q000295:instrumentation* / Q000379:methods; D000882:Haplorhini / Q000033:anatomy & histology / Q000502:physiology / Q000601:surgery*; D008839:Microelectrodes / Q000592:standards*; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D013238:Stereotaxic Techniques / Q000295:instrumentation*; D013594:Syringes / Q000592:standards*