title: The role of preoptic area and anterior hypothalamus and median raphe nucleus on thermoregulatory system in freely moving rats.
abstract: To clarify the role of the preoptic area and anterior hypothalamus (PO/AH) on thermoregulatory system and the effects of serotonergic innervation from the median raphe nucleus (MRN) on body temperature (Tb), we perfused tetrodotoxin (TTX) solution into the PO/AH or MRN by using a microdialysis technique at different ambient temperatures (5, 23 and 35 degrees C) in freely moving rats. Tb was continuously monitored by using a telemetry system. In the MRN, perfusion of TTX solution induced significant hypothermia in the normal environment, a greater decrease in Tb during cold exposure and had no effect on Tb during heat exposure. In the PO/AH, perfusion of TTX solution induced significant hyperthermia in normal environment, a greater increase in Tb during heat exposure and had no effect on Tb during cold exposure. Our results indicate that the PO/AH regulates mainly heat loss or inhibits the loci regulating heat production. Furthermore, heat production appears to be regulated by other loci receiving serotonergic innervation from the MRN.
authors.affiliation: Department of Biology, Faculty of Science, Tokyo Metropolitan University, 1-1 Minami-ohsawa, Hachioji, 192-0397, Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Ishiwata
authors.forename: H
authors.initials: H
authors.lastname: Hasegawa
authors.forename: M
authors.initials: M
authors.lastname: Yasumatsu
authors.forename: F
authors.initials: F
authors.lastname: Akano
authors.forename: T
authors.initials: T
authors.lastname: Yazawa
authors.forename: M
authors.initials: M
authors.lastname: Otokawa
authors.forename: Y
authors.initials: Y
authors.lastname: Aihara
mesh_terms: D000818:Animals; D007025:Anterior Hypothalamic Nucleus / Q000187:drug effects / Q000502:physiology*; D001833:Body Temperature Regulation / Q000187:drug effects / Q000502:physiology*; D003080:Cold Temperature / Q000009:adverse effects; D005334:Fever / Q000139:chemically induced / Q000503:physiopathology; D006358:Hot Temperature / Q000009:adverse effects; D007035:Hypothermia / Q000139:chemically induced / Q000503:physiopathology; D008297:Male; D017551:Microdialysis; D009068:Movement / Q000187:drug effects / Q000502:physiology; D009434:Neural Pathways / Q000187:drug effects / Q000502:physiology*; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D011149:Pons / Q000187:drug effects / Q000502:physiology*; D011301:Preoptic Area / Q000187:drug effects / Q000502:physiology*; D011903:Raphe Nuclei / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D013779:Tetrodotoxin / Q000494:pharmacology
chemical_list: D013779:Tetrodotoxin