Alteration of cardiovascular and neuronal function in M1 knockout mice.
We used gene targeting to generate mice lacking the M1 muscarinic acetylcholine receptor. These mice exhibit a decreased susceptibility to pilocarpine-induced seizures, loss of regulation of M-current potassium channel activity and of a specific calcium channel pathway in sympathetic neurons, a loss of the positive chronotropic and inotropic responses to the novel muscarinic agonist McN-A-343, and impaired learning in a hippocampal-dependent test of spatial memory.
Hamilton
S E
SE
Department of Pharmacology, University of Washington, Seattle 98195-7750, USA.
Hardouin
S N
SN
Anagnostaras
S G
SG
Murphy
G G
GG
Richmond
K N
KN
Silva
A J
AJ
Feigl
E O
EO
Nathanson
N M
NM
D000818:Animals; D015220:Calcium Channels / Q000378:metabolism*; D002478:Cells, Cultured; D004594:Electrophysiology; D019204:GTP-Binding Proteins / Q000378:metabolism; D018390:Gene Targeting; D006321:Heart / Q000187:drug effects / Q000502:physiology*; D006624:Hippocampus / Q000166:cytology / Q000502:physiology; D006801:Humans; D007858:Learning / Q000502:physiology; D008568:Memory / Q000502:physiology; D051379:Mice; D018345:Mice, Knockout; D018721:Muscarinic Agonists / Q000494:pharmacology; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D010095:Oxotremorine / Q000494:pharmacology; D010862:Pilocarpine / Q000494:pharmacology; D015221:Potassium Channels / Q000378:metabolism*; D051381:Rats; D043643:Receptor, Muscarinic M1; D011976:Receptors, Muscarinic / Q000235:genetics / Q000378:metabolism*; D012640:Seizures / Q000139:chemically induced; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology*; D013687:Telencephalon / Q000166:cytology / Q000502:physiology
D015220:Calcium Channels; D018721:Muscarinic Agonists; D015221:Potassium Channels; D043643:Receptor, Muscarinic M1; D011976:Receptors, Muscarinic; D010862:Pilocarpine; D010095:Oxotremorine; D019204:GTP-Binding Proteins