Two novel CNRs from the CNR gene cluster have molecular features distinct from those of CNR1 to 8.
Cadherin-related neuronal receptor (CNR) family proteins are known as synaptic cadherins and Reelin receptors. Here we have identified two novel mouse CNR genes, CNRc1 and CNRc2, orthologues of human protocadherin (Pcdh) alpha-c1 and Pcdhalpha-c2, respectively. While the variable large exons of CNRc1 and c2 contain six conserved extracellular cadherin repeats (EC1-6) and are linked to the constant exons, both contain several molecular features distinct from CNR1-8. CNRc1 and c2 lack the Arg-Gly-Asp (RGD) sequence that is conserved in the EC1 of CNR1-8, which is necessary for binding to Reelin. The present studies confirm that CNRc1 and c2 failed to immunoprecipitate with Reelin. In addition, the regulation of novel CNR expression patterns during brain development is slightly different from that of CNR1. The identification of these new CNR genes characterized by their distinct extracellular function and expression is indicative of the novel diversity of the processes of brain structuring and synapse regulation.
Takei
Y
Y
Laboratory of Neurobiology and Behavioral Genetics, National Institute for Physiological Sciences, Myodaiji, Okazaki, 444-8585, Japan.
Hamada
S
S
Senzaki
K
K
Mutoh
T
T
Sugino
H
H
Yagi
T
T
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D001921:Brain / Q000196:embryology / Q000378:metabolism; D015820:Cadherins / Q000235:genetics* / Q000378:metabolism; D015816:Cell Adhesion Molecules, Neuronal / Q000378:metabolism; D002460:Cell Line; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry / Q000235:genetics; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics*; D009419:Nerve Tissue Proteins; D009479:Neuropeptides / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny; D011233:Precipitin Tests; D011485:Protein Binding; D000091363:Protocadherins; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011956:Receptors, Cell Surface; D000091042:Reelin Protein; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012697:Serine Endopeptidases
D015820:Cadherins; D015816:Cell Adhesion Molecules, Neuronal; D016326:Extracellular Matrix Proteins; D009419:Nerve Tissue Proteins; D009479:Neuropeptides; C484820:PCDHA4 protein, human; C485131:PCDHA6 protein, human; C113143:Pcdha4 protein, mouse; C113144:Pcdha6 protein, mouse; D000091363:Protocadherins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D000091042:Reelin Protein; D004247:DNA; C000717127:RELN protein, human; C000717128:Reln protein, mouse; D012697:Serine Endopeptidases