Potential intervention of Campylobacter jejuni in the modulation of murine immune response.
Campylobacter jejuni has been reported to produce different toxins that may modulate the immune response in both animals and humans. The effect of C. jejuni enterotoxin on the immune response was investigated in two groups of Balb/c mice. One of them was inoculated intraperitoneally with 1010 colony forming units (CFU) of an enterotoxigenic strain (CCUG 7580), and the second one with a non-enterotoxigenic strain (CCUG 7440). The number of polymorphonuclear (PMN) cells from spleen increased in both enterotoxigenic and non-enterotoxigenic strains as a consequence of C. jejuni infection. Notwithstanding, lymphocyte proliferation stimulated by lipopolysaccharide (LPS) was increased by both enterotoxigenic and non-enterotoxigenic strains. Interleukin-2 (IL-2) production from splenic cells was increased significantly by infection with the enterotoxigenic strain. Both enterotoxigenic and non-enterotoxigenic strains reduced the splenic response to sheep erythrocytes; the response was significantly suppressed for immunoglobulin M (Ig M) and for immunoglobulin G (Ig G) synthesis. These results suggest that C. jejuni is able to modify some components of the immune response in mice, and also that the enterotoxigenic strain has more immunomodulating activity than the non-enterotoxigenic strain.
Pancorbo
P L
PL
University of Jaén, Faculty of Experimental Sciences, Department of Health Sciences, Unit of Microbiology, 23071 Jaén, Spain.
de Pablo
M A
MA
Ortega
E
E
Puertollano
M A
MA
Gallego
A M
AM
Alvarez de Cienfuegos
G
G
D000818:Animals; D000917:Antibody Formation; D001427:Bacterial Toxins / Q000096:biosynthesis; D002169:Campylobacter Infections / Q000276:immunology*; D016123:Campylobacter jejuni / Q000276:immunology* / Q000378:metabolism / Q000472:pathogenicity; D004768:Enterotoxins / Q000096:biosynthesis / Q000502:physiology*; D007136:Immunoglobulins / Q000096:biosynthesis; D007376:Interleukin-2 / Q000096:biosynthesis; D007958:Leukocyte Count; D007962:Leukocytes / Q000276:immunology*; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009000:Monocytes / Q000276:immunology; D009504:Neutrophils / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000276:immunology
D001427:Bacterial Toxins; D004768:Enterotoxins; D007136:Immunoglobulins; D007376:Interleukin-2