title: Fish gonadotropin and thyrotropin receptors: the evolution of glycoprotein hormone receptors in vertebrates.
abstract: We have cloned and characterized, for the first time in fish, two different gonadotropin receptors (GTHR) and a single thyrotropin receptor (TSHR) from amago salmon (Oncorhynchus rhodurus) and Nile tilapia (Oreochromis niloticus). Phylogenetic analyses and intron/exon structure suggest that the two GTHRs in fish are comparable to tetrapod follicle stimulating hormone and luteinizing hormone receptors. Temporal and spatial expression patterns, examined by Northern blot analysis and in situ hybridization, paralleled those seen in mammals and birds. Consequently, genetic and functional divergence of two GTHRs and TSHR probably occurred before the teleost and tetrapod split.
authors.affiliation: Laboratory of Reproductive Biology, National Institute for Basic Biology, 444-8585, Okazaki, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Oba
authors.forename: T
authors.initials: T
authors.lastname: Hirai
authors.forename: Y
authors.initials: Y
authors.lastname: Yoshiura
authors.forename: T
authors.initials: T
authors.lastname: Kobayashi
authors.forename: Y
authors.initials: Y
authors.lastname: Nagahama
mesh_terms: D000818:Animals; D019143:Evolution, Molecular; D005399:Fishes / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D011967:Receptors, Gonadotropin / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D011989:Receptors, Thyrotropin / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D014714:Vertebrates / Q000235:genetics*
chemical_list: D011967:Receptors, Gonadotropin; D011989:Receptors, Thyrotropin