title: Mitochondrial ATP-sensitive potassium channel plays a dominant role in ischemic preconditioning of rabbit heart.
abstract: BACKGROUND
  The ATP-sensitive potassium (K(ATP)) channel has been shown to be important in the ischemic preconditioning (IPC) response. Recently, the mitochondrial rather than the sarcolemmal K(ATP) channel has been focused on due to its energy-modulating property. Hence, this study was undertaken to elucidate the role of the mitochondrial K(ATP) channel in IPC by modulating the mitochondrial K(ATP) channel in isolated perfused rabbit hearts.
  
  
  METHODS
  Seven hearts served as a control with no interventions. Seven hearts underwent IPC consisting of two 5-min cycles of global ischemia followed by 5 min of reperfusion. Seven hearts received the selective mitochondrial K(ATP) channel blocker 5-dehydroxydecanoate (5-HD, 100 microM) for 5 min before IPC, and 7 hearts received the selective mitochondrial K(ATP) channel opener diazoxide (50 microM) for 5 min. Then, all hearts were subjected to 1 h of left anterior descending coronary artery ischemia and 1 h of reperfusion. Left ventricular pressures, monophasic action potentials and coronary flow were measured throughout the experiment and infarct size was detected at the end of experiment.
  
  
  RESULTS
  (1) The mitochondria-selective K(ATP) channel opener diazoxide reduced infarct size as compared to control (p < 0.05); (2) IPC reduced infarct size and preserved postischemic diastolic function as compared to control (p < 0.05), and (3) the mitochondria-selective K(ATP) channel blocker 5-HD reversed these effects.
  
  
  CONCLUSIONS
  The mitochondrial ATP-sensitive potassium channel may be a potential site of cardioprotection.
authors.affiliation: Department of Thoracic and Cardiovascular Surgery, Osaka Medical College, Takatsuki, Japan. tho028@poh.osaka-med.ac.jp
authors.forename: Y
authors.initials: Y
authors.lastname: Nakai
authors.forename: H
authors.initials: H
authors.lastname: Horimoto
authors.forename: S
authors.initials: S
authors.lastname: Mieno
authors.forename: S
authors.initials: S
authors.lastname: Sasaki
mesh_terms: D000200:Action Potentials / Q000502:physiology; D000255:Adenosine Triphosphate / Q000502:physiology*; D000818:Animals; D003326:Coronary Circulation; D003971:Diastole; D004594:Electrophysiology; D008297:Male; D008929:Mitochondria, Heart / Q000378:metabolism*; D009203:Myocardial Infarction / Q000473:pathology; D015221:Potassium Channels / Q000502:physiology*; D011312:Pressure; D011817:Rabbits; D011930:Reaction Time / Q000502:physiology; D016277:Ventricular Function, Left
chemical_list: D015221:Potassium Channels; D000255:Adenosine Triphosphate