title: Location of O-acetyl groups in S-657 using the reductive-cleavage method.
abstract: A two-step procedure is described in this paper to identify the position of O-acetyl groups in S-657 polysaccharide. Reductive-cleavage experiments performed on the fully methylated (base-catalyzed) polysaccharide, followed by acetylation of anhydroalditols, identified individual sugar residues and their position of linkage. In a second experiment, the polysaccharide was methylated under neutral conditions leaving native acetate groups intact. Reductive cleavage of the neutral methylated polysaccharide using CF3SO3SiMe3 as a catalyst, followed by acetylation in situ, identified sugar residues containing native acetate groups and established their position of substitution. Using this two-step procedure of analysis, S-657 polysaccharide is shown to contain O-acetyl groups on the 2-position and the 2,6-positions of 3-linked glucopyranosyl residues.
authors.affiliation: CP Kelco, San Diego, CA 92123, USA.
authors.forename: S
authors.initials: S
authors.lastname: Diltz
authors.forename: S G
authors.initials: SG
authors.lastname: Zeller
mesh_terms: D000107:Acetylation; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002849:Chromatography, Gas; D006868:Hydrolysis; D008722:Methods; D008745:Methylation; D008969:Molecular Sequence Data; D015394:Molecular Structure; D010084:Oxidation-Reduction; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D020578:Sphingomonas / Q000737:chemistry
chemical_list: D011135:Polysaccharides, Bacterial; C071624:S 657