title: A chemically defined medium for the growth of Cowdria ruminantium.
abstract: Chemically defined media, termed SFMC-23 and SFMC-36, were devised for the in vitro culture of Cowdria ruminantium, the causative agent of heartwater in domestic ruminants. Both media were based on Dulbecco's modified Eagle's medium nutrient mixture Ham F-12 (DME/F-12) containing various supplements. Medium SFMC-23 and SFMC-36 supported the long-term growth of the Welgevonden stock of C. ruminantium for a total of 55 and 28 passages, respectively, with regular passage intervals of 3 days. Using SFMC-23, split ratios varied from 5-10, depending on which host cell line was used. Other stocks of C. ruminantium (Sankat, Blaauwkrantz, Senegal) were successfully propagated for a test period of ten passages.
authors.affiliation: Parasitology Division, Onderstepoort Veterinary Institute, South Africa.
authors.forename: E
authors.initials: E
authors.lastname: Zweygarth
authors.forename: A I
authors.initials: AI
authors.lastname: Josemans
mesh_terms: D000818:Animals; D001431:Bacteriological Techniques; D002460:Cell Line; D016895:Culture Media, Serum-Free / Q000737:chemistry*; D016995:Ehrlichia ruminantium / Q000254:growth & development*; D004730:Endothelium, Vascular / Q000166:cytology / Q000382:microbiology; D006357:Heartwater Disease / Q000382:microbiology; D012418:Ruminants
chemical_list: D016895:Culture Media, Serum-Free