title: Estrogen receptors alpha and beta exhibit different estradiol and estrogen response element binding in the presence of nonspecific DNA.
abstract: Estrogen receptors (ER) alpha and beta bind estradiol (E(2)) and estrogen response element (ERE) DNA sequences with high affinities. The different migration of ER--ERE complexes in the presence or absence of nonspecific DNA suggests that DNA may affect ER conformation and function. We measured the rate of E(2)--ER association and specific ER--ERE binding capacity (ERE--SBC) in the presence or absence of nonspecific DNA. Whereas DNA did not alter the rate of E(2)--ER alpha association, both ERE-containing and plasmid DNA decreased the rate of E(2) association with ER beta. Poly(dI-dC) decreased ERE--SBC of ER alpha, but did not affect the ERE--SBC of ER beta. Salmon sperm genomic DNA decreased the ERE--SBC of ER alpha, but increased the ERE--SBC of ER beta. We speculate that interaction of ER with genomic DNA may contribute to ER activation and play a role in the observed differences in transcriptional activity of ER alpha and ER beta.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Louisville School of Medicine, Louisville, Kentucky 40292, USA.
authors.forename: V V
authors.initials: VV
authors.lastname: Tyulmenkov
authors.forename: C M
authors.initials: CM
authors.lastname: Klinge
mesh_terms: D001672:Biocompatible Materials / Q000494:pharmacology; D004247:DNA / Q000187:drug effects / Q000378:metabolism*; D017886:Durapatite / Q000494:pharmacology; D004958:Estradiol / Q000378:metabolism*; D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D004967:Estrogens / Q000378:metabolism*; D015894:Genome, Human; D006801:Humans; D007700:Kinetics; D010957:Plasmids / Q000235:genetics; D011960:Receptors, Estrogen / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D012097:Repressor Proteins / Q000378:metabolism
chemical_list: D001672:Biocompatible Materials; D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D004967:Estrogens; D011960:Receptors, Estrogen; D011994:Recombinant Proteins; D012097:Repressor Proteins; D004958:Estradiol; D004247:DNA; D017886:Durapatite