Genome sequence of a VP2/NS junction region of pillar cell necrosis virus (PCNV) in cultured Japanese eel Anguilla japonica.
Pillar cell necrosis virus (PCNV) is an aquatic birnavirus that was isolated from farmed Japanese eel experiencing mass mortality. In this study, a VP2/NS junction region in genome segment A of PCNV was amplified by reverse transcriptase polymerase chain reaction (RT-PCR) and sequenced. The VP2/NS region in PCNV had the highest homology with that of a strain Ab of infectious pancreatic necrosis virus (IPNV). This result revealed that PCNV belongs to birnavirus genogroup II.
Lee
N S
NS
Department of Aquatic Life Medicine, Pukyong National University, Nam-gu, Pusan, Korea. namsill@yahoo.co.kr
Miyata
M
M
Miyazaki
T
T
D000811:Anguilla / Q000821:virology*; D000818:Animals; D001483:Base Sequence; D018059:Birnaviridae / Q000235:genetics*; D003001:Cloning, Molecular; D005393:Fish Diseases / Q000821:virology*; D016679:Genome, Viral; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D017361:Viral Nonstructural Proteins / Q000235:genetics*
D017361:Viral Nonstructural Proteins