title: Structural characterisation of the exopolysaccharide produced by Streptococcus thermophilus EU20.
abstract: Streptococcus thermophilus EU20 when grown on skimmed milk secretes a high-molecular-weight exopolysaccharide that is composed of glucose, galactose and rhamnose in a molar ratio of 2:3:2. Using chemical techniques and 1D and 2D-NMR spectroscopy (1H and 13C) the polysaccharide has been shown to possess a heptasaccharide repeating unit having the following structure: [chemical structure: see text]. Treatment of the polysaccharide with mild acid (0.5 M TFA, 100 degrees C for 1 h) liberates two oligosaccharides; the components correspond to the repeating unit and a hexasaccharide equivalent to the repeating unit minus the terminal alpha-L-Rhap.
authors.affiliation: Division of Food and Nutrition, University of Huddersfield, UK.
authors.forename: V M
authors.initials: VM
authors.lastname: Marshall
authors.forename: H
authors.initials: H
authors.lastname: Dunn
authors.forename: M
authors.initials: M
authors.lastname: Elvin
authors.forename: N
authors.initials: N
authors.lastname: McLay
authors.forename: Y
authors.initials: Y
authors.lastname: Gu
authors.forename: A P
authors.initials: AP
authors.lastname: Laws
mesh_terms: D002240:Carbohydrate Sequence; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D009844:Oligosaccharides / Q000737:chemistry*; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D017421:Sequence Analysis; D013291:Streptococcus / Q000737:chemistry*
chemical_list: D009844:Oligosaccharides; D011135:Polysaccharides, Bacterial; C098632:exopolysaccharide, Streptococcus