Novel findings in inhibition of mast cell-dependent immediate-type cutaneous reactions by Gahmi-Shini-San.
This report describes an inhibitory effect of Gahmi-Shini-San (GSS) on mast cell-mediated immediate-type allergic reactions. GSS is an Oriental herbal medication, which has been successfully used in Korea for the treatment of allergic disorders, mainly skin anaphylactic diseases. GSS inhibited the ear swelling response induced by intradermal injection of compound 48/80 in a mouse model on a concentration-dependent basis. The mast cells in mouse ear tissue were stained by alcian blue/nuclear fast red. GSS significantly inhibited the compound 48/80-induced degranulation from mast cells in ear tissue. GSS dose-dependently inhibited the histamine release from the rat peritoneal mast cells by compound 48/80. We also studied the effect of GSS on mast cell-dependent passive cutaneous anaphylaxis activated by dinitrophenyl IgE antibody. GSS showed inhibition of passive cutaneous anaphylaxis following oral administration. These results indicated that GSS has inhibitory effect on mast cell-dependent immediate type cutaneous reactions.
Hong
S H
SH
Department of Oriental Pharmacy, College of Pharmacy, and Center of Oriental Medicinal Science, Wonkwang University, Chonbuk, 570-749, Iksan, South Korea.
Kim
M S
MS
Lee
J Y
JY
Hwang
C Y
CY
Baek
S H
SH
Han
D S
DS
Jung
W Y
WY
Seo
S B
SB
Kajiuchi
T
T
Kim
H M
HM
D000423:Alcian Blue / Q000032:analysis; D000818:Animals; D015550:Cell Degranulation / Q000187:drug effects*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004423:Ear / Q000503:physiopathology; D006632:Histamine / Q000378:metabolism*; D006967:Hypersensitivity / Q000188:drug therapy / Q000503:physiopathology*; D008407:Mast Cells / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D009499:Neutral Red / Q000032:analysis; D010323:Passive Cutaneous Anaphylaxis / Q000187:drug effects* / Q000502:physiology; D010529:Peritoneal Cavity / Q000166:cytology; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D003189:p-Methoxy-N-methylphenethylamine / Q000037:antagonists & inhibitors
D010936:Plant Extracts; D009499:Neutral Red; D003189:p-Methoxy-N-methylphenethylamine; D006632:Histamine; D000423:Alcian Blue