title: Hybridation of different chiral separation techniques with ICP-MS detection for the separation and determination of selenomethionine enantiomers: chiral speciation of selenized yeast.
abstract: Enantioseparation and determination of selenomethionine enantiomers in selenized yeast was investigated using chiral separation techniques based on different principles, coupled on-line to inductively coupled plasma mass spectrometry (ICP-MS) for selenium-specific detection. High performance liquid chromatography (HPLC) on a beta-cyclodestrin (beta-CD) column, cyclodextrin-modified micellar electrokinetic chromatography (CD-MEKC), gas chromatography (GC) on a Chirasil-L-Val column, and HPLC on a Chirobiotic T column have been investigated as the chiral separation techniques. For HPLC separation on the beta-CD column, and also for CD-MEKC, selenomethionine enantiomers were derivatized with NDA/CN(-). For chiral separation by GC, selenomethionine enantiomers were converted into their N-trifluoroacetyl (TFA)-O-alkyl esters. The developed hybridation methodologies are compared with respect to enantioselectivity, sensitivity and analysis time. The usefulness of the best-suited method [HPLC (Chirobiotic T)-ICP-MS] was demonstrated by its application to the successful chiral speciation of selenium and D-and L-selenomethionine content determination in selenized yeast.
authors.affiliation: Department of Physical and Analytical Chemistry. University of Oviedo, C/Julián Clavería, 8, 33006- Oviedo, Spain.
authors.forename: S P
authors.initials: SP
authors.lastname: Méndez
authors.forename: E B
authors.initials: EB
authors.lastname: González
authors.forename: A
authors.initials: A
authors.lastname: Sanz-Medel
mesh_terms: D002849:Chromatography, Gas / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D020374:Chromatography, Micellar Electrokinetic Capillary; D003505:Cyclodextrins; D019075:Electrophoresis, Capillary / Q000379:methods; D013058:Mass Spectrometry / Q000379:methods*; D012643:Selenium / Q000378:metabolism*; D012645:Selenomethionine / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D013237:Stereoisomerism; D015003:Yeasts / Q000737:chemistry* / Q000378:metabolism; D047392:beta-Cyclodextrins
chemical_list: D003505:Cyclodextrins; D047392:beta-Cyclodextrins; D012645:Selenomethionine; D012643:Selenium; C031215:betadex