Detection of P2X purinergic receptors on human B lymphocytes.
B lymphocytes are known to synthesise the P2X7 subtype of the P2X purinergic receptor family; however, the identification of the other six P2X subtypes on these cells has been limited by the absence of specific antibodies. In this study, we used a panel of anti-P2X polyclonal antibodies and confocal microscopy to examine the presence of each P2X receptor on human B lymphocytes. We observed that P2X1, P2X2, P2X4 and P2X7 subtypes, but not P2X3, P2X5 and P2X6 subtypes, are present on B lymphocytes.
Sluyter
R
R
Department of Medicine, University of Sydney, Nepean Hospital, Penrith, NSW, Australia.  rons@med.usyd.edu.au
Barden
J A
JA
Wiley
J S
JS
D000255:Adenosine Triphosphate / Q000378:metabolism; D000906:Antibodies / Q000378:metabolism; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D003429:Cross Reactions; D000939:Epitopes; D006801:Humans; D007150:Immunohistochemistry; D015640:Ion Channel Gating / Q000502:physiology; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000097:blood*; D018613:Microscopy, Confocal; D011983:Receptors, Purinergic / Q000032:analysis*; D018048:Receptors, Purinergic P2 / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D058469:Receptors, Purinergic P2X; D013329:Structure-Activity Relationship
D000906:Antibodies; D000939:Epitopes; D011983:Receptors, Purinergic; D018048:Receptors, Purinergic P2; D058469:Receptors, Purinergic P2X; D000255:Adenosine Triphosphate