[Characteristics of bacterial lipopolysaccharides depending on extraction method].
Carbohydrate-containing biopolymers have been isolated from Erwinia carotovora subsp. atroseptica 549 by two methods--the aqueous-phenol and with using physiological solution--with addition and without addition of ethylenediaminetetraacetate (EDTA). The biopolymers yield from the cells of bacteria are shown to depend on the extraction method. Lipopolysaccharide-protein complex have been isolated by the sparing method. The purest lipopolysaccharide have been isolated by the aqueousphenol method. The preliminary treatment of cells by EDTA increased the biopolymers output. Carbohydrate containing biopolymers isolated from the cells of bacteria by different methods possess the similar qualitative composition of monosaccharides but they differ in the quantitative content of monosaccharides, spectrum of fatty acids of lipid components as well as in the protein content.
Zherebylo
O Ie
OIe
Zabolotny Institute of Microbiology and Virology, NAS of Ukraine, Kyiv.
Moroz
S M
SM
Hvozdiak
R I
RI
D001704:Biopolymers; D004492:Edetic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000032:analysis; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D016973:Pectobacterium carotovorum / Q000737:chemistry*
D001704:Biopolymers; D005227:Fatty Acids; D008070:Lipopolysaccharides; D004492:Edetic Acid