Differential expression of the lung resistance-related protein/major vault protein in the histological compartments of nephroblastomas.
Nephroblastomas (Wilms' tumors) are curable with survival rates above 80%. Some tumors, however, fail to respond to therapy and those patients have a poor prognosis. In a search for molecular markers of drug resistance, we investigated the expression of lung resistance protein (LRP) in tissue samples from 32 children with nephroblastoma by means of immunohistochemistry. LRP is a human major vault protein (MVP) and is associated with multidrug resistance of tumors. LRP/MVP expression was found in the blastemal and epithelial compartments but to a significantly lesser extent in the stromal compartment of Wilms' tumors. Expression was generally heterogeneous with respect to staining intensity and percentage of positive cells. We found significant relationships between LRP/MVP expression and chemotherapeutic pre-treatment of tumors and tumor stage. The immunohistochemical results were validated with a real-time RT-PCR technique and a significant association between protein and mRNA expression was observed.
Efferth
T
T
Virtual Campus Rhineland-Palatinate, D-55033 Mainz, Germany. efferth@vcrp.de
Bode
M E
ME
Schulten
H G
HG
Thelen
P
P
Granzen
B
B
Beniers
A J
AJ
Mertens
R
R
Gefeller
O
O
Ringert
R H
RH
Jakse
G
G
Fuzesi
L
L
D000903:Antibiotics, Antineoplastic / Q000627:therapeutic use; D000972:Antineoplastic Agents, Phytogenic / Q000627:therapeutic use; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D002648:Child; D002675:Child, Preschool; D017931:DNA Primers / Q000737:chemistry; D003609:Dactinomycin / Q000627:therapeutic use; D018432:Drug Resistance, Multiple; D006801:Humans; D007124:Immunoenzyme Techniques; D007680:Kidney Neoplasms / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020394:Vault Ribonucleoprotein Particles / Q000235:genetics / Q000378:metabolism*; D014750:Vincristine / Q000627:therapeutic use; D009396:Wilms Tumor / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D001613:beta 2-Microglobulin / Q000378:metabolism; D000099187:Major Vault Protein
D000903:Antibiotics, Antineoplastic; D000972:Antineoplastic Agents, Phytogenic; D014408:Biomarkers, Tumor; D017931:DNA Primers; D003609:Dactinomycin; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D020394:Vault Ribonucleoprotein Particles; D014750:Vincristine; D001613:beta 2-Microglobulin; D000099187:Major Vault Protein