A buckwheat protein product suppresses 1,2-dimethylhydrazine-induced colon carcinogenesis in rats by reducing cell proliferation.
This study was conducted to examine the effect of consumption of buckwheat protein product (BWP) on 1,2-dimethylhydrazine (DMH)-induced colon tumor in rats. Male growing Sprague-Dawley rats were fed diets containing either casein or BWP (net protein level, 200 g/kg; n = 20/group) for 124 d. The rats were gavaged weekly with DMH (20 mg/kg body) for the first 8 wk. Food intake and growth were unaffected by dietary manipulation. Dietary BWP caused a 47% reduction in the incidence of colonic adenocarcinoma (P < 0.05), but did not affect the incidence of colonic adenomas. BWP intake tended to reduce the number of colon adenocarcinomas (P = 0.16). Consumption of BWP significantly reduced cell proliferation and expression of c-myc and c-fos proteins in colonic epithelium. The results suggest that dietary BWP has a protective effect against DMH-induced colon carcinogenesis in rats by reducing cell proliferation.
Liu
Z
Z
Department of Applied Biochemistry, Hiroshima University, Higashi-Hiroshima 739-8528, Japan.
Ishikawa
W
W
Huang
X
X
Tomotake
H
H
Kayashita
J
J
Watanabe
H
H
Kato
N
N
D019813:1,2-Dimethylhydrazine; D000818:Animals; D001835:Body Weight; D002273:Carcinogens; D002455:Cell Division / Q000187:drug effects; D003110:Colonic Neoplasms / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D004044:Dietary Proteins / Q000008:administration & dosage / Q000494:pharmacology*; D004435:Eating; D019612:Fagopyrum; D008297:Male; D010940:Plant Proteins / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D002273:Carcinogens; D004044:Dietary Proteins; D010940:Plant Proteins; D019813:1,2-Dimethylhydrazine