Column-switching chromatographic determination of itraconazole and its metabolite hydroxy-itraconazole in human serum.
A column-switching HPLC assay is described for the determination of the antifungal agent itraconazole and its main metabolite hydroxy-itraconazole in serum samples. Three precolumns packed with alkyl-diol silica sorbents differing in alkyl chain length are compared in the sample clean-up step. Chromatographic separation is achieved with a Symmetry C8 column. The assay presented shows a robust and selective analytical procedure with low requirements for sample quantity, no manual sample treatment and high sample throughput.
Kubalec
P
P
Department of Analytical Chemistry, Slovak Technical University, Bratislava, Slovakia.
Brandsteterová
E
E
D000935:Antifungal Agents / Q000097:blood*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007202:Indicators and Reagents; D017964:Itraconazole / Q000031:analogs & derivatives* / Q000097:blood*; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
D000935:Antifungal Agents; D007202:Indicators and Reagents; C084794:hydroxyitraconazole; D017964:Itraconazole