[Activity of cathepsin B and cystatins in the placenta during EPH-gestosis].
Activity of cathepsin B using Bz-DL-Arg-pNA contents of SH-group by means of Ellman method, activity of cystatins against papain using casein as a substrate and contents of deoxyribonuclein acids by Burton method were determined in 64 placentas of pregnancies with EPH-gestosis and in 36 placentas of physiological pregnancies. The placentas from pregnancies with EPH-gestosis showed markedly higher activity of cathepsin B, no difference in the contents of SH-group, slightly higher activity of cystatins and they contain less deoxyribonucleic acids than the placentas from physiological pregnancies. The obtained results show that proteolytic--anti-proteolytic balance in placentas from pregnacies with EPH-gestosis is changed to the advantage of cathepsin B. This protease may in formation of structural and functional changes observed in placentas during EPH-gestosis.
al-Hameri
M
M
Zakładu Analizy Instrumentalnej, Akademii Medycznej w Białymstoku.
Roszkowska-Jakimiec
W
W
Gacko
M
M
Chlabicz
M
M
D002401:Cathepsin B / Q000378:metabolism*; D015891:Cystatins / Q000378:metabolism*; D005260:Female; D006801:Humans; D010920:Placenta / Q000378:metabolism*; D011225:Pre-Eclampsia / Q000378:metabolism*; D011247:Pregnancy
D015891:Cystatins; D002401:Cathepsin B