[Apoptosis and necrosis of neuron after focal ischemia in brain of rats].
This article reports the apoptosis and necrosis of neurons following ischemia-reperfusion in the brain of rats. The focal ischemia model was established by occluding the middle cerebral artery for 2 h and reperfusing for 0.5-48 h. The neuronal changes were investigated by HE staining, TUNEL and agarose gel-electrophoresis. The foci of ischemia were in the preoptic area, striatum and cortex. At 0.5 h of reperfusion, there were quite a few apoptotic neurons in the preoptic ischemic focus, but only a few scattered apoptotic cells were seen in the striatum and cortex. During 3-6 h of reperfusion, the number of apoptotic cells increased and the necrotic cells appeared. The number of apoptotic cells reached a peak at 12-24 h and the in morphology varied, and they were mainly located in the inner boundary of infarct. At 48 h, the apoptotic cells decreased; and the necrotic cells increased. Agarose gel-electrophoresis showed the dense smear and ladder pattern of DNA fragments, the apoptosis and necrosis of neurons coexisted, and apoptosis contributed to the enlargement of the ischemic infarct.
Yuan
Q
Q
Department of Anatomy, School of Basic Medical Sciences, WCUMS, Chengdu 610041.
Li
R
R
Zhang
G
G
Yang
H
H
Chen
H
H
D000818:Animals; D017209:Apoptosis; D002545:Brain Ischemia / Q000473:pathology*; D002540:Cerebral Cortex / Q000473:pathology*; D003342:Corpus Striatum / Q000473:pathology; D008297:Male; D009336:Necrosis; D009474:Neurons / Q000473:pathology; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000473:pathology*