[Comparison of denaturing high performance liquid chromatography with direct sequencing in the detection of single nucleotide polymorphism].
OBJECTIVE
Investigate the sensitivity and accuracy of the denaturing high performance liquid chromatography(DHPLC) technique for the detection of single nucleotide polymorphism(SNP).


METHODS
Forty-one samples were detected by both DHPLC and direct sequencing.


RESULTS
The comparison demonstrated that DHPLC detected all heterozygous sequences found by direct sequencing. No false-positive signals were seen in the cases of homozygous sequences. Furthermore, no false-negative results were ever obtained with heterozygous mutations or polymorphisms, or both.


CONCLUSIONS
DHPLC is a potent method for SNP identification especially SNP typing in large scale screening.
Shi
J
J
Chinese Human Genome Center at Shanghai, Shanghai 201203 P. R. China. huangwei@chgc.sh.cn
Yang
S
S
Jiang
Z
Z
Jiang
H
H
Chen
T
T
Chen
Z
Z
Huang
W
W
D002851:Chromatography, High Pressure Liquid; D006801:Humans; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA