title: Tissue distribution of the human CD97 EGF-TM7 receptor.
abstract: CD97 is a founding member of the EGF-TM7 family of class II seven-span transmembrane (7-TM) receptors. CD97 has an extended extracellular region with several N-terminal epidermal growth factor (EGF)-like domains, which mediate binding to CD55. Previous studies demonstrated the expression of CD97 on activated lymphocytes, monocytes, macrophages, granulocytes, and numerous haematopoietic and nonhaematopoietic cell lines. Here, we determined the cellular distribution of human CD97 in situ by immunohistochemistry (IH) and immunofluorescence (IF). Abundant expression of CD97 was detected on all types of macrophages and dendritic cells, except for microglia. Within the lymphoid lineage, most T cells but only a few B cells express CD97. Germinal centre B cells do not express the molecule. Except for smooth muscle cells, no staining was found on other cells outside the immune system. However, analysis of a restricted set of epithelial tumors revealed CD97 expression on the malignant cells in thyroid and gastrointestinal tract cancer.
authors.affiliation: Department of Pathology, Academic Hospital, Free University, Amsterdam, The Netherlands.
authors.forename: L H
authors.initials: LH
authors.lastname: Jaspars
authors.forename: W
authors.initials: W
authors.lastname: Vos
authors.forename: G
authors.initials: G
authors.lastname: Aust
authors.forename: R A
authors.initials: RA
authors.lastname: Van Lier
authors.forename: J
authors.initials: J
authors.lastname: Hamann
mesh_terms: D000911:Antibodies, Monoclonal / Q000378:metabolism; D015703:Antigens, CD; D001666:Binding Sites, Antibody; D018958:CD55 Antigens / Q000096:biosynthesis / Q000276:immunology / Q000378:metabolism; D002277:Carcinoma / Q000276:immunology; D004815:Epidermal Growth Factor / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D008221:Lymphoid Tissue / Q000166:cytology / Q000276:immunology; D008562:Membrane Glycoproteins / Q000096:biosynthesis / Q000276:immunology / Q000378:metabolism*; D009130:Muscle, Smooth / Q000166:cytology / Q000276:immunology; D022423:Myeloid Cells / Q000276:immunology; D009928:Organ Specificity / Q000276:immunology*; D043562:Receptors, G-Protein-Coupled
chemical_list: C095004:ADGRE5 protein, human; D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D018958:CD55 Antigens; D008562:Membrane Glycoproteins; D043562:Receptors, G-Protein-Coupled; D004815:Epidermal Growth Factor