Desulfurization of benzothiophene by the Gram-negative bacterium, Sinorhizobium sp. KT55.
Sinorhizobium sp. KT55 was the first Gram-negative isolate to be capable of utilizing benzothiophene as the sole source of sulfur. By GC-MS analysis of metabolites of benzothiophene by this strain, benzothiophene sulfone, benzo[e][1,2]oxathiin S-oxide and o-hydroxystyrene were detected, suggesting that the benzothiophene desulfurization pathway of this strain is benzothiophene-->benzothiophene sulfoxide-->benzothiophene sulfone-->benzo[e][1,2]oxathiin S-oxide-->o-hydroxystyrene. Desulfurization activity of this strain was significantly repressed by methionine, cysteine, sulfate, dimethyl sulfoxide, and Casamino acids.
Tanaka
Y
Y
Tsukuba Branch of Bio-Refining Process Laboratory, Advanced Technology and Research Institute, Japan Petroleum Energy Center, 1-1 Higashi Tsukuba, Ibaraki 305-8566, Japan. spweek@nibh.go.jp
Onaka
T
T
Matsui
T
T
Maruhashi
K
K
Kurane
R
R
D000596:Amino Acids / Q000378:metabolism; D001673:Biodegradation, Environmental; D003470:Culture Media; D004121:Dimethyl Sulfoxide / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D020579:Sinorhizobium / Q000254:growth & development / Q000378:metabolism*; D013455:Sulfur / Q000378:metabolism; D013876:Thiophenes / Q000378:metabolism*
D000596:Amino Acids; D003470:Culture Media; D013876:Thiophenes; C088015:benzothiophene; D013455:Sulfur; D004121:Dimethyl Sulfoxide