Effects of deflected droplet electrostatic cell sorting on the viability and exoproteolytic activity of bacterial cultures and marine bacterioplankton.
The cell-sorting capability of flow cytometers makes it possible to isolate specific populations of cells with pre-defined cytometric characteristics. A better knowledge of the biological effects of the sorting process is necessary for the future cell sorting applications. In this paper we report the effects of flow cytometric sorting on bacterial viability and exoproteolytic activity (EPA) of bacterial cultures and marine bacterioplankton. Sorting bacterial cultures and bacterioplankton samples reduce viability as assessed by plate counts and produce variations in the exoproteolytic activity. These effects indicate that deflected electrostatic sorting may significantly alter the biological properties of the sorted bacteria.
Resina-Pelfort
O
O
Departament de Microbiologia, Universitat de Barcelona, Spain.
Comas-Riu
J
J
Vives-Rego
J
J
D000818:Animals; D001386:Azides / Q000633:toxicity; D001419:Bacteria / Q000166:cytology* / Q000302:isolation & purification; D002469:Cell Separation / Q000379:methods*; D015169:Colony Count, Microbial; D004121:Dimethyl Sulfoxide / Q000633:toxicity; D020689:Exopeptidases / Q000032:analysis; D005434:Flow Cytometry / Q000379:methods*; D008386:Marine Biology / Q000379:methods*; D010933:Plankton / Q000166:cytology / Q000302:isolation & purification; D055672:Static Electricity; D014871:Water Microbiology
D001386:Azides; C010596:Isoton; D020689:Exopeptidases; D004121:Dimethyl Sulfoxide