title: Mechanical properties and structure of carotid arteries in mice lacking desmin.
abstract: OBJECTIVE
  Our aim was to determine in desmin homozygous mutant mice the viscoelastic properties, the mechanical strength and the structure of the carotid artery.
  
  
  METHODS
  To assess the viscoelastic properties of large arteries, we have performed an in vivo analysis of the diameter-, and distensibility-pressure curves of the common carotid artery (CCA) in homozygous (Des -/-), heterozygous (Des +/-) and wild-type (Des +/+) mice. To evaluate the mechanical strength, we have measured the in vitro intraluminal pressure producing the rupture of the carotid artery wall. The structure analysis of the arterial wall was based on histology and electronic microscopy.
  
  
  RESULTS
  A lower distensibility and an increase of arterial wall viscosity were observed in Des -/- compared with Des +/+. Arterial thickness of Des -/- was similar to those of Des +/+, without changes in elastin and collagen contents. Electron microscopy revealed that the perimeter of cellular fingerlike-projections was smaller in Des -/-, indicating that the cells have lost part of their connections to the extracellular matrix. The rupture pressure was significantly lower in Des -/- (1500+/-200 mmHg) compared with Des +/+ (2100+/-80 mmHg) indicating a lower mechanical strength of the vascular wall. No significant difference was found between Des +/- and Des +/+.
  
  
  CONCLUSIONS
  The desmin is essential to maintain proper viscoelastic properties, structure and mechanical strength of the vascular wall.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale, INSERM U337, 15 Rue de l'Ecole de Médecine, 75270 Paris Cedex 06, France. lacolley@ccr.jussieu.fr
authors.forename: P
authors.initials: P
authors.lastname: Lacolley
authors.forename: P
authors.initials: P
authors.lastname: Challande
authors.forename: S
authors.initials: S
authors.lastname: Boumaza
authors.forename: G
authors.initials: G
authors.lastname: Cohuet
authors.forename: S
authors.initials: S
authors.lastname: Laurent
authors.forename: P
authors.initials: P
authors.lastname: Boutouyrie
authors.forename: J A
authors.initials: JA
authors.lastname: Grimaud
authors.forename: D
authors.initials: D
authors.lastname: Paulin
authors.forename: J M
authors.initials: JM
authors.lastname: Lamazière
authors.forename: Z
authors.initials: Z
authors.lastname: Li
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001011:Aorta / Q000737:chemistry; D001696:Biomechanical Phenomena; D015153:Blotting, Western; D017536:Carotid Artery, Common / Q000000981:diagnostic imaging / Q000502:physiology* / Q000648:ultrastructure; D003893:Desmin / Q000032:analysis / Q000172:deficiency* / Q000235:genetics; D004548:Elasticity; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D051379:Mice; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D008854:Microscopy, Electron; D009131:Muscle, Smooth, Vascular / Q000737:chemistry / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014463:Ultrasonography; D014746:Vimentin / Q000032:analysis; D014783:Viscosity
chemical_list: D003893:Desmin; D014746:Vimentin