Autoradiographic distribution of adrenomedullin receptors in the rat brain.
The autoradiographic distribution of putative brain adrenomedullin receptors was investigated using [125I]human adrenomedullin(13-52) as a new radioligand. Specific [125I]human adrenomedullin(13-52) binding sites were very discretely distributed in the rat brain with enrichment seen in the choroid plexus and linings of the third, fourth and lateral ventricles, basolateral amygdaloid nuclei, neural lobe of the pituitary gland, the trigeminal nerves and in the granular cell layer of the cerebellum. To our knowledge, this is the first report on the discrete localization of adrenomedullin receptors in the mammalian brain.
Juaneda
C
C
Douglas Hospital Research Centre and Department of Psychiatry, McGill University, 6875 La Salle Boulevard, H4H 1R3, Verdun, QC, Canada.
Dumont
Y
Y
Chabot
J G
JG
Quirion
R
R
D053607:Adrenomedullin; D000818:Animals; D001345:Autoradiography; D001667:Binding, Competitive; D001921:Brain / Q000378:metabolism*; D007457:Iodine Radioisotopes; D008297:Male; D010446:Peptide Fragments / Q000378:metabolism; D011869:Radioligand Assay; D051381:Rats; D017207:Rats, Sprague-Dawley; D058265:Receptors, Adrenomedullin; D018000:Receptors, Peptide / Q000378:metabolism*
D007457:Iodine Radioisotopes; D010446:Peptide Fragments; D058265:Receptors, Adrenomedullin; D018000:Receptors, Peptide; C094546:adrenomedullin (13-52); D053607:Adrenomedullin