Organization of human ACAT-2 gene and its cell-type-specific promoter activity.
Acyl-CoA:cholesterol acyltransferase (ACAT) plays important roles in cellular cholesterol homeostasis. Two ACAT genes exist in mammals. We report here the genomic organization of human ACAT-2 gene and analysis of its promoter activity in various cell lines. The human ACAT-2 gene spans over 18 kb and contains 15 exons. Three transcription start sites and one poly(A) site are identified by the 5'/3'-RACE. In addition, the human ACAT-2 gene is linked to the insulin-like growth factor binding protein 6 (IGFBP-6) gene in a head-to-tail manner with a small intergenic region of about 1.2 kb. The 5'-flanking region of human ACAT-2 gene contains many potential cis-acting elements for multiple transcriptional regulatory factors but lacks TATA and CCAAT boxes. Using promoter-luciferase reporter assays, we demonstrate the transcriptional activity of ACAT-2 gene promoter is high in Caco-2 cells, especially after these cells become postconfluent and behave as intestinal enterocytes.
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Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai, 200031, China. boliang@server.shcnc.ac.cn
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D001483:Base Sequence; D018938:Caco-2 Cells; D002460:Cell Line; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D017930:Genes, Reporter; D008040:Genetic Linkage; D006801:Humans; D018976:Insulin-Like Growth Factor Binding Protein 6 / Q000235:genetics; D007527:Isoenzymes / Q000235:genetics; D008156:Luciferases / Q000235:genetics; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D002785:Sterol O-Acyltransferase / Q000235:genetics*; D014158:Transcription, Genetic; D000097587:Sterol O-Acyltransferase 2
D004247:DNA; D017931:DNA Primers; D018976:Insulin-Like Growth Factor Binding Protein 6; D007527:Isoenzymes; D008156:Luciferases; D002785:Sterol O-Acyltransferase; D000097587:Sterol O-Acyltransferase 2