title: Vibrio cholerae tolC is required for bile resistance and colonization.
abstract: TolC and its homologues are outer membrane proteins that are essential for the transport of many molecules across the cell envelope. In this study we characterized the gene encoding Vibrio cholerae TolC. V. cholerae tolC mutants failed to secrete the RTX cytotoxin, were hypersensitive to antimicrobial agents, and were deficient in intestinal colonization.
authors.affiliation: Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA.
authors.forename: J E
authors.initials: JE
authors.lastname: Bina
authors.forename: J J
authors.initials: JJ
authors.lastname: Mekalanos
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D001427:Bacterial Toxins / Q000378:metabolism*; D001646:Bile / Q000276:immunology*; D001647:Bile Acids and Salts / Q000494:pharmacology; D004352:Drug Resistance, Microbial; D029968:Escherichia coli Proteins; D006460:Hemolysin Proteins; D006801:Humans; D026901:Membrane Transport Proteins; D051379:Mice; D016254:Mutagenesis, Insertional; D014407:Tumor Cells, Cultured; D014734:Vibrio cholerae / Q000187:drug effects / Q000254:growth & development* / Q000276:immunology / Q000472:pathogenicity
chemical_list: D000900:Anti-Bacterial Agents; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D001647:Bile Acids and Salts; D029968:Escherichia coli Proteins; D006460:Hemolysin Proteins; D026901:Membrane Transport Proteins; C073126:tolC protein, E coli; C087744:ApxI toxin, Bacteria