Rat liver responsiveness to gluconeogenic substrates during insulin-induced hypoglycemia.
Hepatic responsiveness to gluconeogenic substrates during insulin-induced hypoglycemia was investigated. For this purpose, livers were perfused with a saturating concentration of 2 mM glycerol, 5 mM L-alanine or 5 mM L-glutamine as gluconeogenic substrates. All experiments were performed 1 h after an ip injection of saline (CN group) or 1 IU/kg of insulin (IN group). The IN group showed higher (P<0.05) hepatic glucose production from glycerol, L-alanine and L-glutamine and higher (P<0.05) production of L-lactate, pyruvate and urea from L-alanine and L-glutamine. In addition, ip injection of 100 mg/kg glycerol, L-alanine and L-glutamine promoted glucose recovery. The results indicate that the hepatic capacity to produce glucose from gluconeogenic precursors was increased during insulin-induced hypoglycemia.
de Souza
H M
HM
Departamento de Ciências Fisiológicas, Universidade Estadual de Londrina, Londrina, PR, Brazil.
Borba-Murad
G R
GR
Ceddia
R B
RB
Curi
R
R
Vardanega-Peicher
M
M
Bazotte
R B
RB
D000409:Alanine / Q000097:blood / Q000494:pharmacology; D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D003451:Cryoprotective Agents / Q000494:pharmacology; D005943:Gluconeogenesis / Q000187:drug effects*; D005947:Glucose / Q000096:biosynthesis; D005973:Glutamine / Q000097:blood / Q000494:pharmacology; D005990:Glycerol / Q000097:blood / Q000494:pharmacology; D007003:Hypoglycemia / Q000139:chemically induced / Q000378:metabolism*; D007004:Hypoglycemic Agents; D007328:Insulin; D019344:Lactic Acid / Q000096:biosynthesis; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D019289:Pyruvic Acid / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D014508:Urea / Q000378:metabolism
D001786:Blood Glucose; D003451:Cryoprotective Agents; D007004:Hypoglycemic Agents; D007328:Insulin; D005973:Glutamine; D019344:Lactic Acid; D019289:Pyruvic Acid; D014508:Urea; D005947:Glucose; D000409:Alanine; D005990:Glycerol