title: Developmental program expression of myosin alkali light chain and skeletal actin genes in the rainbow trout Oncorhynchus mykiss.
abstract: We have isolated MLC1(F) (tMLC1(F)), MLC3(F) (tMLC3(F)) and skeletal actin cDNAs from the teleost Oncorhynchus mykiss. Sequence analysis indicates that tMLC1(F) and tMLC3(F) are not produced from differentially spliced mRNAs as reported in avians and rodents but are encoded by different genes. Results from RNase protection analysis showed that the corresponding transcripts are expressed in fast skeletal muscles. Whole-mount in situ hybridisation revealed distinct expression patterns of the myosin alkali light chains and skeletal actin genes during skeletal muscle development in the embryo.
authors.affiliation: Unité INSERM 441, Université Bordeaux, Pessac, France. pierre.thiebaud@bordeaux.inserm.fr
authors.forename: P
authors.initials: P
authors.lastname: Thiébaud
authors.forename: P Y
authors.initials: PY
authors.lastname: Rescan
authors.forename: W
authors.initials: W
authors.lastname: Barillot
authors.forename: C
authors.initials: C
authors.lastname: Rallière
authors.forename: N
authors.initials: N
authors.lastname: Thézé
mesh_terms: D000199:Actins / Q000096:biosynthesis / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D018076:DNA, Complementary / Q000096:biosynthesis / Q000302:isolation & purification; D004625:Embryo, Nonmammalian / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000196:embryology / Q000378:metabolism*; D018994:Myosin Light Chains / Q000096:biosynthesis / Q000235:genetics*; D017686:Oncorhynchus mykiss / Q000196:embryology / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D013045:Species Specificity
chemical_list: D000199:Actins; D018076:DNA, Complementary; D018994:Myosin Light Chains; D012333:RNA, Messenger