Location of O-acetyl groups in S-657 using the reductive-cleavage method.
A two-step procedure is described in this paper to identify the position of O-acetyl groups in S-657 polysaccharide. Reductive-cleavage experiments performed on the fully methylated (base-catalyzed) polysaccharide, followed by acetylation of anhydroalditols, identified individual sugar residues and their position of linkage. In a second experiment, the polysaccharide was methylated under neutral conditions leaving native acetate groups intact. Reductive cleavage of the neutral methylated polysaccharide using CF3SO3SiMe3 as a catalyst, followed by acetylation in situ, identified sugar residues containing native acetate groups and established their position of substitution. Using this two-step procedure of analysis, S-657 polysaccharide is shown to contain O-acetyl groups on the 2-position and the 2,6-positions of 3-linked glucopyranosyl residues.
Diltz
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CP Kelco, San Diego, CA 92123, USA.
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D000107:Acetylation; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002849:Chromatography, Gas; D006868:Hydrolysis; D008722:Methods; D008745:Methylation; D008969:Molecular Sequence Data; D015394:Molecular Structure; D010084:Oxidation-Reduction; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D020578:Sphingomonas / Q000737:chemistry
D011135:Polysaccharides, Bacterial; C071624:S 657