Poly(A)-associated RNA in plants.
The RNA associated with poly(A) sequences from Euglena gracilis and Vicia faba has been isolated by binding to millipore filters and characterized by sedimentation velocity centrifugation and electrophoretic mobility. Poly(A)-associated RNA as isolated in solution was highly aggregated. When denatured, it sedimented as a broad peak with a mean value of 16-18 S. This RNA was shown to be covalently linked to poly(A) sequences which are 150-250 nucleotides long. Our size estimates for plant poly(A) and poly(A)-associated RNA are similar to those obtained for animal cells.
Sagher
D
D
Department of Plant Genetics, Weizmann Institute of Science, Rehovot, Israel.
Edelman
M
M
Jakob
K M
KM
D000818:Animals; D001482:Base Composition; D005056:Euglena gracilis; D010944:Plants / Q000235:genetics*; D012333:RNA, Messenger / Q000737:chemistry; D018749:RNA, Plant / Q000737:chemistry / Q000302:isolation & purification*
D012333:RNA, Messenger; D018749:RNA, Plant