Integrin regulation of receptor tyrosine kinase and G protein-coupled receptor signaling to mitogen-activated protein kinases.
Assays for use in integrin-mediated or -modulated signaling are essentially the same as those used in signaling studies involving growth factors or hormones. The major differences are the manipulations of the cells to compare the effects of gain or loss of anchorage, or the role of specific adhesion receptors. This needs to be done with some care, and with thought given to the overall biology of the particular cell type under investigation. Harsh treatment of cells, for example, prolonged suspension culture, may result in irreversible nonphysiological effects in some types of cells.
Juliano
R L
RL
Department of Pharmacology, School of Medicine, University of North Carolina, Chapel Hill, North Carolina 27599-7365, USA.
Aplin
A E
AE
Howe
A K
AK
Short
S
S
Lee
J W
JW
Alahari
S
S
D016475:3T3 Cells; D000818:Animals; D000906:Antibodies; D002118:Calcium / Q000378:metabolism; D002448:Cell Adhesion; D002478:Cells, Cultured; D004789:Enzyme Activation; D005455:Fluorescent Antibody Technique; D019204:GTP-Binding Proteins / Q000378:metabolism*; D006801:Humans; D016023:Integrins / Q000032:analysis / Q000276:immunology / Q000502:physiology*; D020935:MAP Kinase Signaling System; D051379:Mice; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D011233:Precipitin Tests; D019908:Proto-Oncogene Proteins c-raf / Q000378:metabolism; D020794:Receptor Protein-Tyrosine Kinases / Q000378:metabolism / Q000502:physiology*; D011956:Receptors, Cell Surface / Q000378:metabolism / Q000502:physiology*; D014162:Transfection
D000906:Antibodies; D016023:Integrins; D011956:Receptors, Cell Surface; D020794:Receptor Protein-Tyrosine Kinases; D019908:Proto-Oncogene Proteins c-raf; D020928:Mitogen-Activated Protein Kinases; D019204:GTP-Binding Proteins; D002118:Calcium