Effect of LPS administration on the expression of POMC, NPY, galanin, CART and MCH mRNAs in the rat hypothalamus.
Anorexia and weight loss are manifestations of inflammation seen both in patients and in experimental animal models such as the lipopolysaccharide (LPS)-treated rat. Using in situ hybridization, the levels of mRNAs encoding proopiomelanocortin (POMC), neuropeptide Y (NPY), galanin, melanin-concentrating hormone (MCH) and cocaine- and amphetamine-regulated transcript (CART) were investigated in the rat hypothalamus after a single intraperitoneal dose (125 microg/kg) of LPS. Four hours after LPS injection the food intake was significantly decreased. POMC and CART mRNA levels were increased in the arcuate nucleus, and MCH, CART and galanin mRNAs were all decreased in the lateral hypothalamic area in LPS-treated rats. Levels of mRNAs for NPY and galanin in the arcuate nucleus, and for MCH and CART in the zona incerta did not change significantly after LPS treatment. These findings support the hypothesis that LPS-induced factors mediate signalling to the POMC/CART neurons in the arcuate nucleus which could lead to reduced food intake by decreasing MCH, CART and galanin synthesis in target lateral hypothalamic neurons.
Sergeyev
V
V
Department of Neuroscience, Karolinska Institutet, S-171 77, Stockholm, Sweden.
Broberger
C
C
Hökfelt
T
T
D000818:Animals; D000855:Anorexia / Q000503:physiopathology; D001111:Arcuate Nucleus of Hypothalamus / Q000502:physiology*; D004435:Eating / Q000187:drug effects / Q000502:physiology; D019004:Galanin / Q000235:genetics*; D015870:Gene Expression / Q000187:drug effects; D007026:Hypothalamic Area, Lateral / Q000502:physiology; D007028:Hypothalamic Hormones / Q000235:genetics; D017403:In Situ Hybridization; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008297:Male; D008543:Melanins / Q000235:genetics; D009419:Nerve Tissue Proteins / Q000235:genetics; D009478:Neuropeptide Y / Q000235:genetics*; D010907:Pituitary Hormones / Q000235:genetics; D011333:Pro-Opiomelanocortin / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D000098882:Cocaine- and Amphetamine-Regulated Transcript Protein
D007028:Hypothalamic Hormones; D008070:Lipopolysaccharides; D008543:Melanins; D009419:Nerve Tissue Proteins; D009478:Neuropeptide Y; D010907:Pituitary Hormones; D012333:RNA, Messenger; D000098882:Cocaine- and Amphetamine-Regulated Transcript Protein; D011333:Pro-Opiomelanocortin; C019463:melanin-concentrating hormone; D019004:Galanin