title: Molecular characterization of a novel plasmid-encoded cefotaximase (CTX-M-12) found in clinical Klebsiella pneumoniae isolates from Kenya.
abstract: Nine Klebsiella pneumoniae isolates, six from blood and three from cerebrospinal fluid of newborn babies at Kenyatta National Hospital, Nairobi, Kenya, were analyzed for the mechanism of cephalosporin resistance. By using pulsed-field gel electrophoresis of XbaI-digested chromosomal DNA, all the nine isolates were found to be clonal. PCR and direct sequencing revealed a novel extended-spectrum beta-lactamase, which we designated CTX-M-12. It has a more potent hydrolytic activity against cefotaxime than against ceftazidime and a pI of 9.0 and is encoded on a large self-transferable ca. 160-kbp plasmid.
authors.affiliation: Centre for Microbiology Research, Kenya Medical Research Institute, Nairobi, Kenya. skariuki@wtrl.or.ke
authors.forename: S
authors.initials: S
authors.lastname: Kariuki
authors.forename: J E
authors.initials: JE
authors.lastname: Corkill
authors.forename: G
authors.initials: G
authors.lastname: Revathi
authors.forename: R
authors.initials: R
authors.lastname: Musoke
authors.forename: C A
authors.initials: CA
authors.lastname: Hart
mesh_terms: D019943:Amino Acid Substitution; D002439:Cefotaxime / Q000378:metabolism; D002511:Cephalosporins / Q000378:metabolism; D019143:Evolution, Molecular; D006801:Humans; D007630:Kenya; D007711:Klebsiella pneumoniae / Q000201:enzymology* / Q000235:genetics; D008969:Molecular Sequence Data; D001618:beta-Lactamases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D002511:Cephalosporins; C071167:beta-lactamase CTX-2; D001618:beta-Lactamases; D002439:Cefotaxime