Effects of acute handling stress on whitefish Coregonus lavaretus after prolonged exposure to biologically treated and untreated bleached kraft mill effluent.
Exposure of fish to water of impaired quality has been shown to disrupt the function of the hypothalamo-pituitary-interrenal (HPI) axis and alter the interpretation of data from field studies due to the varying effects of handling and delayed sampling on exposed and reference animals. In the present study, juvenile whitefish, Coregonus lavaretus, were exposed for 6 weeks to diluted (4-8%) untreated and biologically treated bleached kraft mill effluent (BKME) and their response to acute handling was investigated. Liver microsomal EROD activity and glycogen phosphorylase (GPase) activity, in addition to gill Na+-K+-ATPase activity, and blood hemoglobin and hematocrit levels were increased in whitefish exposed for 6 weeks to untreated BKME, whereas those exposed to treated BKME exhibited increased blood hemoglobin and red blood cell K+ concentrations. Both handling procedures, exposure to a shallow water (10 cm, 5 min) and to an air challenge (10 s air/10 s water/30 s air/10 s water/10 s air), resulted in acute physiological stress, as recorded after 5-, 60-, and 120-min recovery periods. Following air exposure, the levels of plasma cortisol, blood glucose, hemoglobin, and hematocrit as well as the liver GPase activity were increased, and liver glycogen concentration decreased in control fish. These responses were attenuated in fish exposed to untreated or treated BKME. Plasma estradiol and testosterone levels were not affected by the BKME exposures or by the air challenge. Handling also resulted in attenuated EROD induction in fish exposed to untreated BKME. According to the present findings, the sensitivity of some widely used cellular and physiological variables may be improved by time-dependent standardization when interpreting data obtained following delayed sampling.
Lappivaara
J
J
Department of Biological and Environmental Science, University of Jyväskylä, P.O. Box 35, FIN-40351 Jyväskylä, Finland. jrmo.lappivaara@uku.fi
D000822:Animal Husbandry; D000818:Animals; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism; D004790:Enzyme Induction; D005399:Fishes / Q000502:physiology*; D006400:Hematocrit; D006454:Hemoglobins / Q000032:analysis*; D010209:Paper; D006005:Phosphorylases / Q000378:metabolism*; D015203:Reproducibility of Results; D013048:Specimen Handling; D013997:Time Factors; D014865:Waste Disposal, Fluid; D014874:Water Pollutants, Chemical / Q000009:adverse effects*
D006454:Hemoglobins; D014874:Water Pollutants, Chemical; D019363:Cytochrome P-450 CYP1A1; D006005:Phosphorylases