Plasma concentrations of estradiol 17beta and PGF2alpha metabolite and placental fatty acid composition and antioxidant enzyme activity in cows with and without retained fetal membranes.
Fetal membrane retention is one of the most common problems in Holstein cattle after parturition. To investigate mechanisms involved, the following parameters were studied in the peri-parturition period: plasmatic concentrations of estradiol-17beta (E2) and PGFM (PGF2alpha metabolite), activity of antioxidant enzymes (superoxide dismutase-SOD, catalase-CAT and glutathione peroxidase-GSH-Px), thiobarbituric acid reagent substances (TBAR) concentrations and fatty acid composition of the placentae. E2 at parturition in the NPR group (control cows, n = 10) was higher than in PR cows (placental retention, n = 10) (P < 0.05). Activity of SOD in fetal tissue of NPR animals was higher than that of the PR group. In contrast, there was no difference between the two groups in activity of GSH-Px and CAT and the TBAR content of placental tissues. PR maternal tissues had proportionally more arachidonic and linoleic acid than tissues from NPR cows. Therefore, a complex of sequential events may cause placenta retention, starting with an unbalance of antioxidant capacity of the placenta, followed by a decrease in production of estrogen, which leads to the accumulation of arachidonic and linoleic acid in placental tissues.
Wischral
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Department of Veterinary Medicine, Rural Federal University of Pernambuco, Recife, PE, Brazil. aureaman@elogica.com.br
Nishiyama-Naruke
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Curi
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Barnabe
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RC
D000818:Animals; D000975:Antioxidants / Q000032:analysis*; D002374:Catalase / Q000378:metabolism; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood*; D015237:Dinoprost / Q000031:analogs & derivatives / Q000097:blood*; D004958:Estradiol / Q000097:blood*; D005227:Fatty Acids / Q000032:analysis*; D005260:Female; D005979:Glutathione Peroxidase / Q000378:metabolism; D007743:Labor, Obstetric / Q000097:blood; D010920:Placenta / Q000737:chemistry; D018457:Placenta, Retained / Q000097:blood / Q000662:veterinary*; D011247:Pregnancy; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis
D000975:Antioxidants; D005227:Fatty Acids; D017392:Thiobarbituric Acid Reactive Substances; C008228:15-keto-13,14-dihydroprostaglandin F2alpha; D004958:Estradiol; D015237:Dinoprost; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase