Biodiversity of Anabaena azollae isolates from different Azolla cultures.
The random amplified polymorphic DNA (RAPD) profile of A. azollae strains isolated from four different Azolla cultures was studied by using different primers. The objective of this study was to determine whether polymerase chain reaction (PCR) with different primers could differentiate the isolated A. azollae strains from one another. The primers amplified specific sequences of the isolates and generated fingerprinting pattern characteristic of each isolate. Clear polymorphism was noticed among all the strains which depends on the primer sequence.
Subhashini
R
R
Department of Agricultural Microbiology, Tamil Nadu Agricultural University, Coimbatore 641 003, India.
Kumar
K
K
Kannaiyan
S
S
D017033:Anabaena / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D001483:Base Sequence; D017931:DNA Primers; D010944:Plants / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D019105:Random Amplified Polymorphic DNA Technique
D017931:DNA Primers