Molecular population genetic analysis of Campylobacter jejuni HS:19 associated with Guillain-Barré syndrome and gastroenteritis.
Infection with Campylobacter jejuni serotype HS:19 is associated with the development of Guillain-Barré syndrome (GBS). To determine whether a particular HS:19 clone is associated with GBS, multilocus enzyme electrophoresis (MLEE) was used to analyze a worldwide collection of isolates. There were 34 electropherotypes (ETs) in 3 phylogenetic clusters among 83 C. jejuni isolates. Cluster I contained all HS:19 strains, and a single ET (ET4) accounted for most HS:19 strains. HS:19 strains did not occur in any of the other clusters. ET4 contained isolates from different geographic locations, indicating global spread of this clone. Furthermore, ET4 contained isolates from patients with uncomplicated enteritis and GBS, as well as isolates from animal sources. The results of this study show that HS:19 strains comprise a clonal, although not monomorphic, population, which is distinct from non-HS:19 strains within C. jejuni. A unique clone associated with GBS was not identified by use of MLEE.
Nachamkin
I
I
Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA 19104-4283, USA. nachamki@mail.med.upenn.edu
Engberg
J
J
Gutacker
M
M
Meinersman
R J
RJ
Li
C Y
CY
Arzate
P
P
Teeple
E
E
Fussing
V
V
Ho
T W
TW
Asbury
A K
AK
Griffin
J W
JW
McKhann
G M
GM
Piffaretti
J C
JC
D002169:Campylobacter Infections / Q000150:complications* / Q000382:microbiology; D016123:Campylobacter jejuni / Q000145:classification / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D002170:Canada; D002681:China; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics*; D003718:Denmark; D004586:Electrophoresis / Q000379:methods; D005759:Gastroenteritis / Q000150:complications* / Q000382:microbiology; D005784:Gene Amplification; D020275:Guillain-Barre Syndrome / Q000382:microbiology*; D006801:Humans; D007564:Japan; D008800:Mexico; D013019:South Africa; D006113:United Kingdom; D014481:United States
D004269:DNA, Bacterial