Fibrous stationary phase in capillary electrochromatography.
Capillary electrochromatography (CEC) using fibrous cellulose acetate (CA) stationary phase was investigated. The advantage of this fiber-packed column is relatively easy preparation process compared with other conventional CEC columns, such as particle-packed and wall-coated capillaries. CA fibers are manually packed into a capillary with two guide liners and fixed with a frit at the column inlet. The separation characteristics of this column were investigated using n-alkyl p-hydroxybenzoates (parabens) as the sample probe. It has been demonstrated that the use of a short column length and a specially designed tee-connector as the injection device should make the separation performance and efficiency much higher on the fiber-packed columns. Sufficient separation between methyl and n-butylparabens is obtained on the 5-cm-packed column and linear relationships between the injection time and the peak area are observed. Bubble formation is not encountered during the analysis.
Jinno
K
K
School of Materials Science, Toyohashi University of Technology, 441-8580, Toyohashi, Japan. jinno@chrom.tutms.tut.ac.jp
Watanabe
H
H
Kiso
Y
Y
D055598:Chemical Phenomena; D002621:Chemistry / Q000379:methods; D002627:Chemistry, Physical; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000295:instrumentation* / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000379:methods*; D010226:Parabens / Q000737:chemistry; D011506:Proteins / Q000302:isolation & purification; D013997:Time Factors
D010226:Parabens; D011506:Proteins