Data quality in predictive toxicology: reproducibility of rodent carcinogenicity experiments.
We compared 121 replicate rodent carcinogenicity assays from the two parts (National Cancer Institute/National Toxicology Program and literature) of the Carcinogenic Potency Database (CPDB) to estimate the reliability of these experiments. We estimated a concordance of 57% between the overall rodent carcinogenicity classifications from both sources. This value did not improve substantially when additional biologic information (species, sex, strain, target organs) was considered. These results indicate that rodent carcinogenicity assays are much less reproducible than previously expected, an effect that should be considered in the development of structure-activity relationship models and the risk assessment process.
Gottmann
E
E
Institute for Cancer Research, Institute for Environmental Hygiene, University Vienna, Vienna, Austria. eva.gottmann@bmbwk.gv.at
Kramer
S
S
Pfahringer
B
B
Helma
C
C
D000818:Animals; D015197:Carcinogenicity Tests / Q000592:standards / Q000706:statistics & numerical data*; D002273:Carcinogens / Q000737:chemistry / Q000633:toxicity*; D016208:Databases, Factual; D005260:Female; D008297:Male; D051379:Mice; D009369:Neoplasms / Q000139:chemically induced*; D011237:Predictive Value of Tests; D051381:Rats; D015203:Reproducibility of Results; D018570:Risk Assessment; D013045:Species Specificity; D013329:Structure-Activity Relationship
D002273:Carcinogens