title: C26-CoA-dependent ceramide synthesis of Saccharomyces cerevisiae is operated by Lag1p and Lac1p.
abstract: Lag1p and Lac1p are two highly homologous membrane proteins of the endoplasmic reticulum (ER). When both genes are deleted, cells cannot transport glycosylphosphatidylinositol (GPI)-anchored proteins from the ER to the Golgi at a normal rate. Here we show that microsomes or detergent extracts from lag1lac1 double mutants lack an activity transferring C26 fatty acids from C26-coenzyme A onto dihydrosphingosine or phytosphingosine. As a consequence, in intact cells, the normal ceramides and inositolphosphorylceramides are drastically reduced. lag1lac1 cells compensate for the lack of normal sphingolipids by making increased amounts of C26 fatty acids, which become incorporated into glycerophospholipids. They also contain 20- to 25-fold more free long chain bases than wild type and accumulate very large amounts of abnormally polar ceramides. They make small amounts of abnormal mild base-resistant inositolphospholipids. The lipid remodelling of GPI-anchored proteins is severely compromised in lag1lac1 double mutants since only few and mostly abnormal ceramides are incorporated into the GPI anchors. The participation of Lag1p and Lac1p in ceramide synthesis may explain their role in determining longevity.
authors.affiliation: Institute of Biochemistry, University of Fribourg, CH-1700 Fribourg, Switzerland.
authors.forename: I
authors.initials: I
authors.lastname: Guillas
authors.forename: P A
authors.initials: PA
authors.lastname: Kirchman
authors.forename: R
authors.initials: R
authors.lastname: Chuard
authors.forename: M
authors.initials: M
authors.lastname: Pfefferli
authors.forename: J C
authors.initials: JC
authors.lastname: Jiang
authors.forename: S M
authors.initials: SM
authors.lastname: Jazwinski
authors.forename: A
authors.initials: A
authors.lastname: Conzelmann
mesh_terms: D000214:Acyl Coenzyme A / Q000378:metabolism*; D002518:Ceramides / Q000096:biosynthesis*; D004721:Endoplasmic Reticulum / Q000378:metabolism; D005227:Fatty Acids / Q000096:biosynthesis*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D005838:Genotype; D017261:Glycosylphosphatidylinositols / Q000378:metabolism; D007700:Kinetics; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D008861:Microsomes / Q000378:metabolism*; D009154:Mutation; D021381:Protein Transport; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins; D013107:Sphingolipids / Q000096:biosynthesis*; D013110:Sphingosine / Q000031:analogs & derivatives* / Q000096:biosynthesis
chemical_list: D000214:Acyl Coenzyme A; D002518:Ceramides; D005227:Fatty Acids; D005656:Fungal Proteins; D017261:Glycosylphosphatidylinositols; C087436:LAG1 protein, S cerevisiae; C101054:LAG2 protein, S cerevisiae; D008565:Membrane Proteins; D029701:Saccharomyces cerevisiae Proteins; D013107:Sphingolipids; C012491:phytosphingosine; D013110:Sphingosine; C005682:safingol