title: Production of a novel compound, 7,10,12-trihydroxy-8(E)-octadecenoic acid from ricinoleic acid by Pseudomonas aeruginosa PR3.
abstract: The production and its potential use of a novel trihydroxy unsaturated fatty acid, 7,10,12-trihydroxy-8(E)-octadecenoic acid (TOD), were investigated. TOD was formed by Pseudomonas aeruginosa PR3 (NRRL B-18602) in a culture supplied with exogenous ricinoleic acid. The yield of TOD production was always higher in a rich culture medium than in minimal screening medium. Extending the conversion time from 48 to 72 h prior to lipid extraction led to a 65% reduction in yield, indicating that TOD was further metabolized by strain PR3 and that control of reaction time is important to achieving a maximum yield. The optimum culture density, reaction time, pH, temperature, and substrate concentration for the production of TOD were: 20-24 h culture growth, 48 h, 7.0, 25 degrees C, and 1% (vol/vol), respectively. Under optimum conditions, the yield of TOD production was greater than 45%. TOD was found to be an antifungal agent most active against the fungus that causes blast disease in rice plants, the most important fungal disease affecting rice production worldwide.
authors.affiliation: Microbial Properties Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, 1815 North University Street, U.S. Department of Agriculture, Peoria, IL 61604, USA. kuotm@mail.ncaur.usda
authors.forename: T M
authors.initials: TM
authors.lastname: Kuo
authors.forename: H
authors.initials: H
authors.lastname: Kim
authors.forename: C T
authors.initials: CT
authors.lastname: Hou
mesh_terms: D003470:Culture Media; D005658:Fungi / Q000187:drug effects; D005659:Fungicides, Industrial / Q000378:metabolism / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D006880:Hydroxy Acids / Q000378:metabolism* / Q000494:pharmacology; D009829:Oleic Acids / Q000378:metabolism* / Q000494:pharmacology; D011550:Pseudomonas aeruginosa / Q000254:growth & development / Q000378:metabolism*; D012277:Ricinoleic Acids / Q000378:metabolism*; D013696:Temperature
chemical_list: C432089:7,10,12-trihydroxy-8-octadecenoic acid; D003470:Culture Media; D005659:Fungicides, Industrial; D006880:Hydroxy Acids; D009829:Oleic Acids; D012277:Ricinoleic Acids; C030521:ricinoleic acid