[Identification of seeds of Cuscuta australis and C. chinensis by TLC and HPLC].
Identification of seeds of Cuscuta australis R. Br. and C. chinensis Lam. was carried out by TLC and HPLC. Polyamide membrane was used as stationary phase, MeOH-HOAc-H2O and CHCl3-MeOH-HOAc were used as mobile phase for TLC. For HPLC, Hypersil-ODS column was used; the mobile phase was MeOH-0.025 M H3PO4; the flow rate was 1.0 ml.min-1; detection wavelength was 360 nm; and column temperature was 40 degrees C. Both methods represented significant identification characteristics, and were simple, accurate and reproducible.
Ye
M
M
School of Pharmacognosy, Beijing Universty of Traditional Chinese Medicine, 100029.
Zhou
P
P
Yan
Y
Y
Li
Y
Y
Liu
H
H
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002855:Chromatography, Thin Layer / Q000379:methods*; D027722:Convolvulaceae / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005419:Flavonoids / Q000032:analysis*; D044949:Kaempferols; D012639:Seeds / Q000737:chemistry*
D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D044949:Kaempferols; C006552:kaempferol