Source of endothelin-1 in subarachnoid hemorrhage.
Endothelin-1 is the most potent vasoconstrictor known to date. This peptide is believed to play a pathophysiological role in the development of vasospasm, the most important complication of subarachnoid hemorrhage (SAH). In the present study we investigated the release of endothelin-1 in SAH and analyzed the cellular source of this peptide. At a protein and mRNA level we were able to show that endothelin-1 is produced by mononuclear leukocytes. Complementary in vitro studies revealed that aging and subsequent hemolysis of blood is sufficient to induce production of endothelin-1 by mononuclear leukocytes. Thus, cerebrospinal fluid-derived mononuclear leukocytes are a source of endothelin-1 in patients suffering from SAH. This finding may have important therapeutic implications as anti-leukocyte strategies could prevent cerebrovascular complications in SAH patients.
Bertsch
T
T
Department of Clinical Chemistry, Clinic Mannheim, University of Heidelberg, Germany.
Kuehl
S
S
Muehlhauser
F
F
Walter
S
S
Hodapp
B
B
Rossol
S
S
Schmeck
J
J
Ragoschke
A
A
Schütt
S
S
Fritzinger
M
M
Horn
P
P
Vajkoczy
P
P
Wellner
M W
MW
Fassbender
K
K
D000328:Adult; D000368:Aged; D000783:Aneurysm / Q000097:blood / Q000134:cerebrospinal fluid / Q000175:diagnosis; D001783:Blood Flow Velocity; D002545:Brain Ischemia / Q000097:blood / Q000134:cerebrospinal fluid / Q000175:diagnosis; D019332:Endothelin-1 / Q000097:blood* / Q000378:metabolism*; D005260:Female; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013345:Subarachnoid Hemorrhage / Q000378:metabolism*
D019332:Endothelin-1; D012333:RNA, Messenger