Endothelial cell culture: protocol to obtain and cultivate human umbilical endothelial cells.
Endothelial cells play a key role in prominent immunological and pathological processes such as leukocyte trafficking, inflammation, atheroma or cancer cell metastasis. Umbilical veins are probably the most widely used source for human endothelial cells, since they are more easily available than many other vessels, they are free from any pathological process and they are physiologically more relevant than many established cell lines. Here, we describe a standard protocol for preparation, maintenance and quality control of these cells.
Marin
V
V
INSERM U387, Laboratoire d'immunologie, Hôpital Sainte Marguerite, 270 Bd Sainte Marguerite, 13009 Marseilles, France. marin@marseille.inserm.fr
Kaplanski
G
G
Grès
S
S
Farnarier
C
C
Bongrand
P
P
D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis; D014470:Umbilical Cord / Q000166:cytology*; D019010:Vascular Cell Adhesion Molecule-1 / Q000096:biosynthesis
D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1