title: Effect of altered flow on the pattern of permeability around rabbit aortic branches.
abstract: Uptake of circulating macromolecules by the aortic wall is greater downstream than upstream of branch sites in immature rabbits, but the opposite pattern is seen at later ages. The mature pattern is nitric oxide dependent; we tested whether it is also flow dependent. Intercostal arteries of anesthetized rabbits were occluded, sham operated, or left alone. Uptake of rhodamine-labeled albumin was assessed by quantitative fluorescence microscopy of the sections through the aorta. In mature animals, uptake was higher upstream than downstream of the control and sham-operated branches, but the pattern was reversed at occluded branches. In young animals, uptake was not significantly different between regions upstream and downstream of control, sham-operated, or occluded branches. The absence of the normal immature pattern may reflect an influence of anesthesia and will assist in the elucidation of mechanisms underlying this pattern. The data for mature animals provide the first direct evidence that flow determines permeability near arterial branches and may account for the inverse spatial correlation between shear stress and disease prevalence at branches of adult human arteries.
authors.affiliation: School of Animal and Microbial Sciences, University of Reading, Reading RG6 6AJ, United Kingdom.
authors.forename: T J
authors.initials: TJ
authors.lastname: Staughton
authors.forename: M J
authors.initials: MJ
authors.lastname: Lever
authors.forename: P D
authors.initials: PD
authors.lastname: Weinberg
mesh_terms: D000375:Aging / Q000502:physiology; D000818:Animals; D001011:Aorta / Q000502:physiology*; D001158:Arteries / Q000502:physiology; D002199:Capillary Permeability / Q000502:physiology*; D005456:Fluorescent Dyes; D008297:Male; D008856:Microscopy, Fluorescence; D011817:Rabbits; D012039:Regional Blood Flow / Q000502:physiology; D012235:Rhodamines; D012272:Ribs / Q000294:innervation*; D012709:Serum Albumin / Q000378:metabolism
chemical_list: D005456:Fluorescent Dyes; D012235:Rhodamines; D012709:Serum Albumin