title: Evolutionarily conserved interaction between CstF-64 and PC4 links transcription, polyadenylation, and termination.
abstract: Tight connections exist between transcription and subsequent processing of mRNA precursors, and interactions between the transcription and polyadenylation machineries seem especially extensive. Using a yeast two-hybrid screen to identify factors that interact with the polyadenylation factor CstF-64, we uncovered an interaction with the transcriptional coactivator PC4. Both human proteins have yeast homologs, Rna15p and Sub1p, respectively, and we show that these two proteins also interact. Given evidence that certain polyadenylation factors, including Rna15p, are necessary for termination in yeast, we show that deletion or overexpression of SUB1 suppresses or enhances, respectively, both growth and termination defects detected in an rna15 mutant strain. Our findings provide an additional, unexpected connection between transcription and polyadenylation and suggest that PC4/Sub1p, via its interaction with CstF-64/Rna15p, possesses an evolutionarily conserved antitermination activity.
authors.affiliation: Department of Biological Sciences, Columbia University, New York, NY 10027, USA.
authors.forename: O
authors.initials: O
authors.lastname: Calvo
authors.forename: J L
authors.initials: JL
authors.lastname: Manley
mesh_terms: D017124:Conserved Sequence / Q000235:genetics; D004268:DNA-Binding Proteins; D019143:Evolution, Molecular; D005656:Fungal Proteins / Q000187:drug effects / Q000235:genetics / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D017874:Immediate-Early Proteins; D008565:Membrane Proteins; D009687:Nuclear Proteins / Q000187:drug effects / Q000235:genetics; D010443:Peptide Chain Termination, Translational / Q000502:physiology*; D011061:Poly A / Q000378:metabolism*; D011233:Precipitin Tests; D011485:Protein Binding; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins; D013728:Terminator Regions, Genetic / Q000235:genetics; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000494:pharmacology; D014158:Transcription, Genetic / Q000187:drug effects*; D020798:Two-Hybrid System Techniques; D039221:mRNA Cleavage and Polyadenylation Factors
chemical_list: D004268:DNA-Binding Proteins; D005656:Fungal Proteins; C490043:IFRD2 protein, human; D017874:Immediate-Early Proteins; D008565:Membrane Proteins; D009687:Nuclear Proteins; D016601:RNA-Binding Proteins; D012097:Repressor Proteins; C101206:SUB1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D015534:Trans-Activators; D014157:Transcription Factors; D039221:mRNA Cleavage and Polyadenylation Factors; C512635:RNA15 protein, S cerevisiae; D011061:Poly A