title: Involvement of CD 8+ and CD 3+ lymphocytes in the transport of Eimeria necatrix sporozoites within the intestinal mucosa of chickens.
abstract: The phenotype of cells transporting sporozoites of Eimeria necatrix during a primary infection was determined using a panel of six monoclonal antibodies to various chicken lymphocyte surface markers. Sporozoites and cells harboring them were examined at 8, 12 and 18 h postinfection using two-color immunoflorescence and confocal microscopy. The majority of parasites observed within lymphocytes were found in CD 8 + (15%) or CD 3+ (13-22%) cells at all time periods examined. Smaller numbers were found within deltagamma TCR+ (5%) and alphabeta TCR+ (5%) lymphocytes. No sporozoites were found within CD 4+ or IgM+ lymphocytes at any of the time periods.
authors.affiliation: Department of Pathobiology, University of Guelph, Ontario, Canada.
authors.forename: S E
authors.initials: SE
authors.lastname: Beattie
authors.forename: J R
authors.initials: JR
authors.lastname: Barta
authors.forename: M A
authors.initials: MA
authors.lastname: Fernando
mesh_terms: D000818:Animals; D017252:CD3 Complex / Q000032:analysis; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000469:parasitology*; D002645:Chickens / Q000469:parasitology* / Q000502:physiology; D003048:Coccidiosis / Q000276:immunology / Q000469:parasitology / Q000662:veterinary*; D004539:Eimeria / Q000472:pathogenicity*; D019085:Fluorescent Antibody Technique, Direct; D006790:Host-Parasite Interactions; D007411:Intestinal Diseases, Parasitic / Q000469:parasitology / Q000662:veterinary*; D007413:Intestinal Mucosa / Q000276:immunology / Q000469:parasitology*; D008018:Life Cycle Stages; D016131:Lymphocyte Subsets / Q000276:immunology / Q000469:parasitology; D008297:Male; D018613:Microscopy, Confocal; D010641:Phenotype; D011201:Poultry Diseases / Q000276:immunology / Q000469:parasitology*
chemical_list: D017252:CD3 Complex