title: EndoS, a novel secreted protein from Streptococcus pyogenes with endoglycosidase activity on human IgG.
abstract: Streptococcus pyogenes is an important human pathogen that selectively interacts with proteins involved in the humoral defense system, such as immunoglobulins and complement factors. In this report we show that S.pyogenes has the ability to hydrolyze the chitobiose core of the asparagine-linked glycan on immuno globulin G (IgG) when bacteria are grown in the presence of human plasma. This activity is associated with the secretion of a novel 108 kDa protein denoted EndoS. EndoS has endoglycosidase activity on purified soluble IgG as well as IgG bound to the bacterial surface. EndoS is required for the activity on IgG, as an isogenic EndoS mutant could not hydrolyze the glycan on IgG. In addition, we show that the secreted streptococcal cysteine proteinase SpeB cleaves IgG in the hinge region in a papain-like manner. This is the first example of an endoglycosidase produced by a bacterial pathogen that selectively hydrolyzes human IgG, and reveals a novel mechanism which may contribute to S.pyogenes pathogenesis.
authors.affiliation: Department of Cell and Molecular Biology, Section for Molecular Pathogenesis, BMC-B14, SE-221 84, Lund, Sweden.
authors.forename: M
authors.initials: M
authors.lastname: Collin
authors.forename: A
authors.initials: A
authors.lastname: Olsén
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D002240:Carbohydrate Sequence; D004187:Disaccharides / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D006868:Hydrolysis; D007074:Immunoglobulin G / Q000378:metabolism*; D008969:Molecular Sequence Data; D016296:Mutagenesis; D011134:Polysaccharides / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013297:Streptococcus pyogenes / Q000201:enzymology* / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D004187:Disaccharides; D007074:Immunoglobulin G; D011134:Polysaccharides; C032438:chitobiose; D006026:Glycoside Hydrolases; C431325:NDOS protein, Streptococcus pyogenes