title: Quenching of biotinylated aequorin bioluminescence by dye-labeled avidin conjugates: application to homogeneous bioluminescence resonance energy transfer assays.
abstract: [see reaction]. Avidin conjugates containing the covalently attached dyes QSY-7 and dabcyl were prepared and shown to quench the bioluminescence of biotinylated aequorin. Quenching efficiency was shown to be dependent on both the label-to-avidin ratio and the concentration of the avidin conjugate. These properties were exploited to develop a homogeneous bioluminescence resonance energy transfer (BRET) assay for biotin.
authors.affiliation: Department of Chemistry, Diagnostics Division, Abbott Laboratories, 100 Abbott Park Road, Abbott Park, Illinois 60064-6016, USA. maciej.adamczyk@abbott.com
authors.forename: M
authors.initials: M
authors.lastname: Adamczyk
authors.forename: J A
authors.initials: JA
authors.lastname: Moore
authors.forename: K
authors.initials: K
authors.lastname: Shreder
mesh_terms: D000331:Aequorin / Q000737:chemistry*; D001360:Avidin / Q000737:chemistry*; D001710:Biotin / Q000737:chemistry*; D004735:Energy Transfer; D008163:Luminescent Measurements
chemical_list: D001360:Avidin; D000331:Aequorin; D001710:Biotin