Evidence for gene silencing in Haemophilus influenzae.
Evidence for gene silencing of Haemophilus influenzae involved a beta-subunit of RNA polymerase. The gene presumed silenced was rifampin resistance. The evidence that it was silencing, rather than dominance of a rifampin-sensitive marker, was that it took place when the rifampin resistance marker was on both a plasmid and the chromosome, without the presence of a rifampin-sensitive marker, as judged by lack of transformation of a rifampin-resistant cell to rifampin sensitivity by the plasmid. In addition, three compounds that are known to decrease gene silencing in eukaryotes (trichostatin A, sodium butyrate and 5-azacytidine) also decreased the presumed silencing in H. influenzae. Silencing of rifampin-resistant Escherichia coli did not take place with the plasmid from H. influenzae.
Setlow
J K
JK
Department of Biology, Brookhaven National Laboratory, Upton, NY 11973-5000, USA.
Albritton
W L
WL
Cabrera-Juárez
E
E
D001374:Azacitidine / Q000494:pharmacology; D002087:Butyrates / Q000494:pharmacology; D002701:Chloramphenicol / Q000494:pharmacology; D012321:DNA-Directed RNA Polymerases / Q000235:genetics / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004352:Drug Resistance, Microbial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D005787:Gene Frequency; D020868:Gene Silencing; D006193:Haemophilus influenzae / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D006877:Hydroxamic Acids / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D009154:Mutation; D010957:Plasmids / Q000235:genetics; D012293:Rifampin / Q000494:pharmacology; D014169:Transformation, Bacterial
D002087:Butyrates; D006877:Hydroxamic Acids; C012589:trichostatin A; D002701:Chloramphenicol; D012321:DNA-Directed RNA Polymerases; D001374:Azacitidine; D012293:Rifampin