Detection of herpes simplex and varicella zoster viruses in clinical specimens using direct immunofluorescence and cell culture assays.
Patients (33 in toto) with a clinical diagnosis of herpes infections (simplex, zoster or chickenpox) were investigated for the presence of herpes simplex virus (HSV) and varicella zoster virus (VZV) in skin samples, using direct immunofluorescence and cell culture assays. Five patients with nonherpetic vesiculobullous disorders were included as negative controls. Of the 33 patients, nineteen (57.6%) were positive for HSV or VZV and fourteen (42.4%) were negative. Five controls were all negative for HSV or VZV. Of the nineteen positive patients, HSV was isolated from eight (42.1%) patients, by both direct immunofluorescence and cell culture assays. VZV was isolated from eleven (57.9%) patients, eleven (100%) by direct immunofluorescence assay, and six (54.5%) by cell culture assays. HSV was isolated from one patient clinically diagnosed as chickenpox (VZV), but otherwise the positive laboratory results were concordant with the clinical diagnosis. For epidemiological studies, atypical cases and immunocompromised patients the clinical diagnosis should be confirmed in the laboratory.
Meqdam
M M
MM
Department of Applied Biology, Jordan University of Science and Technology, Irbid.
Todd
D
D
Al-Abosi
M
M
D000914:Antibodies, Viral / Q000032:analysis; D019085:Fluorescent Antibody Technique, Direct; D006561:Herpes Simplex / Q000821:virology*; D006562:Herpes Zoster / Q000821:virology*; D014645:Herpesvirus 3, Human / Q000302:isolation & purification*; D006801:Humans; D007597:Jordan; D018139:Simplexvirus / Q000302:isolation & purification*
D000914:Antibodies, Viral