Sequence comparisons among dispersed members of the Brassica S multigene family in an S9 genome.
Self-incompatibility (SI) systems prevent self-pollination and promote outbreeding. In Brassica, the SI genes SLG (for S-locus glycoprotein) and SRK (for S-receptor kinase) are members of the S multigene family, which share the SLG-like domain (S domain), which encodes a putative receptor. We have cloned members of the S multigene family from the S9 haplotype of B. campestris (syn. rapa). In addition, eight distinct genomic regions harboring 10 SLG/SRK-like genes were characterized in the present study. Sequence analysis revealed two novel SRK-like genes, BcRK3 and BcRK6 (for B. campestris receptor kinases 3 and 6, respectively). Other genes that were characterized included SFR2 (for S gene family receptor 2), SLR2 (for S locus related gene 2), and a pseudogene. Based on phylogenetic analysis of the nucleotide sequences of the S domain regions, SLG and SRK appear to be distinct from other members of the S multigene family. Linkage analysis showed that most members of the S multigene family are dispersed in the Brassica genome, and that SLR1 (S locus related gene 1) is not linked to the SLR2 in B. campestris.
Kai
N
N
Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
Suzuki
G
G
Watanabe
M
M
Isogai
A
A
Hinata
K
K
D015139:Blotting, Southern; D001937:Brassica / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism; D019143:Evolution, Molecular; D008040:Genetic Linkage; D014644:Genetic Variation; D018745:Genome, Plant; D006239:Haplotypes; D006720:Homozygote; D008957:Models, Genetic; D005810:Multigene Family; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D017434:Protein Structure, Tertiary; D017422:Sequence Analysis, DNA
D018076:DNA, Complementary