Antigenic comparison of a truncated form of VP2 of infectious pancreatic necrosis (IPN) virus expressed in four different cell types.
A truncated form of the structural protein VP2 (truncVP2) of infectious pancreatic necrosis (IPN) virus encompassing amino acids 147-307 was expressed in bacterial, yeast, piscine and mammalian cells. All four recombinant antigens were recognised by a VP2-specific monoclonal antibody by ELISA and immunoblot analysis. However, the minimum amount of r-truncVP2 needed for detection by these methods varies depending on the cell type used for expression. Furthermore, all four recombinant preparations, when used to immunise Atlantic salmon, were capable of inducing antibodies reactive with whole IPNV in ELISA.
Labus
M B
MB
Department of Molecular and Cell Biology, University of Aberdeen, Scotland, UK.
Breeman
S
S
Ellis
A E
AE
Smail
D A
DA
Kervick
M
M
Melvin
W T
WT
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D001483:Base Sequence; D018175:Birnaviridae Infections / Q000276:immunology* / Q000517:prevention & control; D015153:Blotting, Western / Q000662:veterinary; D016466:CHO Cells; D002213:Capsid / Q000235:genetics / Q000276:immunology*; D036022:Capsid Proteins; D006224:Cricetinae; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D000939:Epitopes / Q000276:immunology; D004926:Escherichia coli / Q000235:genetics; D007114:Immunization / Q000662:veterinary; D018061:Infectious pancreatic necrosis virus / Q000235:genetics / Q000276:immunology*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D010843:Pichia / Q000235:genetics; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D012474:Salmon / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology / Q000592:standards
D000914:Antibodies, Viral; D000956:Antigens, Viral; D036022:Capsid Proteins; D000939:Epitopes; D010446:Peptide Fragments; D011994:Recombinant Proteins; D014765:Viral Vaccines