Relationship between diurnal rhythm of cell cycle and interferon receptor expression in implanted-tumor cells.
Whether the diurnal rhythm of cell cycle is associated with that of interferon-alpha/beta receptor (IFNAR) expression was investigated in implanted-tumor cells. The expression of IFNAR mRNA significantly increased when the proportion of tumor cells in DNA synthesis (S) phase increased in vitro. A diurnal rhythm was observed for cell cycle distribution in implanted-tumor cells. The specific binding of interferon-alpha to receptor and IFNAR mRNA increased when the proportion of tumor cells in S phase increased in vivo. The time-dependent expression of IFNAR was supported by that of transcription factor level induced by interferon-beta. The present result suggests that the rhythm of IFNAR expression is closely related to that of cell cycle distribution in implanted-tumor cells.
Takane
H
H
Department of Clinical Pharmacokinetics, Graduate School, Kyushu University, Fukuoka, Japan.
Ohdo
S
S
Yamada
T
T
Koyanagi
S
S
Yukawa
E
E
Higuchi
S
S
D000818:Animals; D015153:Blotting, Western; D002453:Cell Cycle / Q000502:physiology*; D002940:Circadian Rhythm / Q000502:physiology*; D005434:Flow Cytometry; D016898:Interferon-alpha / Q000378:metabolism; D016899:Interferon-beta / Q000627:therapeutic use; D008545:Melanoma / Q000188:drug therapy / Q000378:metabolism*; D051379:Mice; D009368:Neoplasm Transplantation; D012333:RNA, Messenger / Q000096:biosynthesis; D053633:Receptor, Interferon alpha-beta; D017471:Receptors, Interferon / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
D016898:Interferon-alpha; D012333:RNA, Messenger; D017471:Receptors, Interferon; D053633:Receptor, Interferon alpha-beta; D016899:Interferon-beta