Tumour enhancement with newly developed Mn-metalloporphyrin (HOP-9P) in magnetic resonance imaging of mice.
The purpose of the study is to evaluate the tumour enhancing characteristics and biodistribution of a newly developed metalloporphyrin derivative, HOP-9P (13, 17-bis (1-carboxypropionyl) carbamoylethyl-3, 8-bis (1-phenylpropyloxyethyl)-2,7,12,18-tetra- methyl-porphynato manganese (III)). Seven mice bearing SCC VII tumours were imaged using T1-weighted conventional spin echo magnetic resonance images before and 5 min, 2 h and 24 h after intravenous injection of 0.1 mmol/kg of HOP-9P. For the acquired images, signal intensities of the tumour, muscle and oil-phantom were measured. Then, tumor/oil and tumor/muscle signal intensity ratios were calculated. Nineteen mice were sacrificed before or after the administration of HOP-9P (at 5 min, 2 h and 24 h), and the biodistribution of manganese in the tumour, muscle, liver, blood and kidneys was measured using optical emission spectrometers and was expressed as micrograms of manganese per gram of tissue. The tumour/muscle signal intensity ratio at 24 h (3.18 +/- 0.34) was significantly higher than precontrast ratio (1.77 +/- 0.20) (P < 0.05). The biodistribution assessment of manganese demonstrated that HOP-9P gradually and consistently accumulated in the tumour to reach the highest concentration at 24 h (3.49 +/- 1.22 micro gMn/g). It is concluded that HOP-9P is a potential tumour-specific MR contrast agent.
Takehara
Y
Y
Department of Radiology, Hamamatsu University School of Medicine, 3600 Handa, Hamamtsu 431-3192, Japan.
Sakahara
H
H
Masunaga
H
H
Isogai
S
S
Kodaira
N
N
Takeda
H
H
Saga
T
T
Nakajima
S
S
Sakata
I
I
D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D003287:Contrast Media / Q000008:administration & dosage / Q000493:pharmacokinetics*; D007275:Injections, Intravenous; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D008345:Manganese; D008665:Metalloporphyrins / Q000008:administration & dosage / Q000493:pharmacokinetics*; D051379:Mice; D009374:Neoplasms, Experimental; D012878:Skin Neoplasms / Q000473:pathology*; D014018:Tissue Distribution
D003287:Contrast Media; C431026:HOP-9P; D008665:Metalloporphyrins; D008345:Manganese