Induction of marine mollusc stress proteins by chemical or physical stress.
The cellular stress responses of most organisms in part involve the induction of a class of proteins called heat shock or stress proteins (HSPs) as a result of damage to existing proteins. Cellular proteins can be damaged by chemical exposures known to induce various HSPs. In these experiments, we examine the HSP responses of mussel (Mytilus galloprovincialis) and abalone (Haliotis rufescens) tissues to both thermal and chemical exposures. HSP70 isoforms, HSP60, and HSP90 all show varying induction capabilities. The results demonstrate that the extent of stress exposure as both a time- and dose-dependent phenomena can be ascertained by examining changes in mollusc HSP protein levels. We also examined the relationship between HSP induction and levels of a mussel cytochrome P450 (CYP4Y1) mRNA in dose-response experiments with the products of biologically degraded weathered crude oil. The increases in HSP70 isoforms and HSP90 were correlated with decreases in CYP4Y1 expression levels in a dose-dependent manner. HSP responses may therefore be a valuable part of a suite of biomarkers in biomonitoring for hydrocarbon exposures in nearshore environments.
Snyder
M J
MJ
Bodega Marine Laboratory, University of California, Davis, P.O. Box 247, Bodega Bay, California, 94923, USA. mjsnyder@ucdavis.edu
Girvetz
E
E
Mulder
E P
EP
D000818:Animals; D060829:Arthropod Proteins; D015415:Biomarkers / Q000032:analysis; D049872:Bivalvia / Q000502:physiology*; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D006838:Hydrocarbons / Q000009:adverse effects*; D008974:Mollusca / Q000502:physiology*; D012333:RNA, Messenger; D014874:Water Pollutants, Chemical / Q000009:adverse effects*
D060829:Arthropod Proteins; D015415:Biomarkers; C400811:CYP41 protein, Boophilus microplus; D006360:Heat-Shock Proteins; D006838:Hydrocarbons; D012333:RNA, Messenger; D014874:Water Pollutants, Chemical; D003577:Cytochrome P-450 Enzyme System