A modified rapid method of nucleic acid isolation from suspension of matured virus: applied in restriction analysis of DNA from an adenovirus prototype strain and a patient isolate.
This report describes a method for the isolation of nucleic acid from a suspension of matured virus. Nucleic acid (DNA) was isolated from a prototype strain of adenovirus type 7 and a clinical isolate of adenovirus type 7. Instead of the usual method of ultracentifugation, a filtration method was applied to concentrate the virus rapidly and nucleic acid was then isolated by a standard phenol/chloroform/isoamyl-alcohol extraction procedure. The DNA was found to be sufficiently purified to generate a reproducible restriction endonuclease digestion pattern. The clinical isolate of adenovirus type 7 revealed loss of restriction site for the endonuclease HindIII when compared with the prototype strain.
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Naval Health Research Center, DoD Center for Deployment Health Research PO Box 85122, San Diego, CA 92186-5122, USA.
Gray
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Naval Health Research Center, DoD Center for Deployment Health Research PO Box 85122, San Diego, CA 92186-5122, USA.
Poddar
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Naval Health Research Center, DoD Center for Deployment Health Research PO Box 85122, San Diego, CA 92186-5122, USA.
D000257:Adenoviridae Infections / Q000821:virology*; D000260:Adenoviruses, Human / Q000235:genetics*; D002478:Cells, Cultured; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004279:DNA, Viral / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D006801:Humans; D015183:Restriction Mapping
D004279:DNA, Viral; D004262:DNA Restriction Enzymes