Immobilization of Candida rugosa lipase on colloidal gas aphrons (CGAs).
A novel technique for immobilization of Candida rugosa lipase onto anionic colloidal gas aphrons (CGAs) is described. CGAs are spherical microbubbles (10-100 microm) composed of an inner gas core surrounded by a surfactant shell. In this initial study, greater than 80% lipase (w/w) was effectively retained on the CGAs. Leakage of protein from the CGAs and the activity of the adsorbed lipase decreased with increasing enzyme loading; this indicates that multilayers of lipase may be adsorbing onto the CGAs. The CGA-immobilised lipase displayed normal Michaelis-Menten dependence on substrate concentration and also exhibited greater activity than the free enzyme.
O'Connell
P J
PJ
Biotechnology and Biochemical Engineering Group, University of Reading, P.O. Box 226, Reading, RG6 6AP, UK.
Varley
J
J
D001709:Biotechnology / Q000379:methods*; D003102:Colloids; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D008049:Lipase / Q000737:chemistry* / Q000378:metabolism
D003102:Colloids; D004800:Enzymes, Immobilized; D008049:Lipase