title: H4(D10S170), a gene frequently rearranged in papillary thyroid carcinoma, is fused to the platelet-derived growth factor receptor beta gene in atypical chronic myeloid leukemia with t(5;10)(q33;q22).
abstract: The molecular cloning of the t(5;10)(q33;q22) associated with atypical chronic myeloid leukemia (CML) is reported. Fluorescence in situ hybridization (FISH), Southern blot, and reverse transcriptase- polymerase chain reaction analysis demonstrated that the translocation resulted in an H4/platelet-derived growth factor receptor betaR (PDGFbetaR) fusion transcript that incorporated 5' sequences from H4 fused in frame to 3' PDGFbetaR sequences encoding the transmembrane, WW-like, and tyrosine kinase domains. FISH combined with immunophenotype analysis showed that t(5;10)(q33;q22) was present in CD13(+) and CD14(+) cells but was not observed in CD3(+) or CD19(+) cells. H4 has previously been implicated in pathogenesis of papillary thyroid carcinoma as a fusion partner of RET. The H4/RET fusion incorporates 101 amino acids of H4, predicted to encode a leucine zipper dimerization domain, whereas the H4/PDGFbetaR fusion incorporated an additional 267 amino acids of H4. Retroviral transduction of H4/PDGFbetaR, but not a kinase-inactive mutant, conferred factor-independent growth to Ba/F3 cells and caused a T-cell lymphoblastic lymphoma in a murine bone marrow transplantation assay of transformation. Mutational analysis showed that the amino-terminal H4 leucine zipper domain (amino acids 55-93), as well as H4 amino acids 101 to 386, was required for efficient induction of factor-independent growth of Ba/F3 cells. Tryptophan-to-alanine substitutions in the PDGFbetaR WW-like domain at positions 566/593, or tyrosine-to-phenylalanine substitutions at PDGFbetaR positions 579/581 impaired factor-independent growth of Ba/F3 cells. H4/PDGFbetaR is an oncoprotein expressed in t(5;10)(q33;q22) atypical CML and requires dimerization motifs in the H4 moiety, as well as residues implicated in signal transduction by PDGFbetaR, for efficient induction of factor-independent growth of Ba/F3 cells. (Blood. 2001;97:3910-3918)
authors.affiliation: Division of Hematology, Department of Medicine, Harvard Medical School, Boston, MA, USA.
authors.forename: J
authors.initials: J
authors.lastname: Schwaller
authors.forename: E
authors.initials: E
authors.lastname: Anastasiadou
authors.forename: D
authors.initials: D
authors.lastname: Cain
authors.forename: J
authors.initials: J
authors.lastname: Kutok
authors.forename: S
authors.initials: S
authors.lastname: Wojiski
authors.forename: I R
authors.initials: IR
authors.lastname: Williams
authors.forename: R
authors.initials: R
authors.lastname: LaStarza
authors.forename: B
authors.initials: B
authors.lastname: Crescenzi
authors.forename: D W
authors.initials: DW
authors.lastname: Sternberg
authors.forename: P
authors.initials: P
authors.lastname: Andreasson
authors.forename: R
authors.initials: R
authors.lastname: Schiavo
authors.forename: S
authors.initials: S
authors.lastname: Siena
authors.forename: C
authors.initials: C
authors.lastname: Mecucci
authors.forename: D G
authors.initials: DG
authors.lastname: Gilliland
mesh_terms: D002291:Carcinoma, Papillary / Q000235:genetics*; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D002879:Chromosomes, Human, Pair 10 / Q000235:genetics; D002895:Chromosomes, Human, Pair 5 / Q000235:genetics; D003001:Cloning, Molecular; D020732:Cytogenetic Analysis; D003598:Cytoskeletal Proteins; D004273:DNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D015321:Gene Rearrangement; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000209:etiology / Q000235:genetics* / Q000473:pathology; D016296:Mutagenesis; D022423:Myeloid Cells / Q000378:metabolism / Q000473:pathology; D015514:Oncogene Proteins, Fusion; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000235:genetics* / Q000378:metabolism; D020797:Receptor, Platelet-Derived Growth Factor beta / Q000235:genetics*; D013964:Thyroid Neoplasms / Q000235:genetics*; D014162:Transfection; D014178:Translocation, Genetic
chemical_list: C088832:CCDC6 protein, human; D003598:Cytoskeletal Proteins; D004273:DNA, Neoplasm; D015514:Oncogene Proteins, Fusion; D011506:Proteins; D020797:Receptor, Platelet-Derived Growth Factor beta