Structural characterisation of the exopolysaccharide produced by Streptococcus thermophilus EU20.
Streptococcus thermophilus EU20 when grown on skimmed milk secretes a high-molecular-weight exopolysaccharide that is composed of glucose, galactose and rhamnose in a molar ratio of 2:3:2. Using chemical techniques and 1D and 2D-NMR spectroscopy (1H and 13C) the polysaccharide has been shown to possess a heptasaccharide repeating unit having the following structure: [chemical structure: see text]. Treatment of the polysaccharide with mild acid (0.5 M TFA, 100 degrees C for 1 h) liberates two oligosaccharides; the components correspond to the repeating unit and a hexasaccharide equivalent to the repeating unit minus the terminal alpha-L-Rhap.
Marshall
V M
VM
Division of Food and Nutrition, University of Huddersfield, UK.
Dunn
H
H
Elvin
M
M
McLay
N
N
Gu
Y
Y
Laws
A P
AP
D002240:Carbohydrate Sequence; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D009844:Oligosaccharides / Q000737:chemistry*; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D017421:Sequence Analysis; D013291:Streptococcus / Q000737:chemistry*
D009844:Oligosaccharides; D011135:Polysaccharides, Bacterial; C098632:exopolysaccharide, Streptococcus