title: Evaluation of scavenging activity assessed by Co(II)/EDTA-induced luminol chemiluminescence and DPPH* (2,2-diphenyl-1-picrylhydrazyl) free radical assay.
abstract: The scavenging activities of three standard antioxidants, quercetin, ascorbic acid, and trolox, were evaluated by Co(II)/ethylenediamine-tetraacetic acid (EDTA)-induced luminol chemiluminescence and the 2,2-diphenyl-1-picrylhydrazyl (DPPH*) free radical assay. Therefore, the aim of this study was to characterise an enzyme-free and time-independent chemiluminescence method for the assessment of the scavenging profile of compounds in a cell-free system using the Co(II)/EDTA-luminol-peroxide system. These results showed that the three standards were efficient and effective in inhibiting both Co(II)/EDTA-induced luminol chemiluminescence and the free radical DPPH*. For all the data obtained in this work, the scavenging activity for the standards tested decreased in the following order: quercetin > trolox > ascorbic acid. The present study has applied a simple and precise procedure for the study of hydroxyl radical scavenging activity by Co(II)/EDTA-induced luminol chemiluminescence, and this was assessed by DPPH* free radical scavenging.
authors.affiliation: Department of Natural Products, Faculty of Pharmacy, University of Barcelona, Avda. Diagonal 643, 08028, Catalonia, Barcelona, Spain.
authors.forename: I
authors.initials: I
authors.lastname: Parejo
authors.forename: C
authors.initials: C
authors.lastname: Codina
authors.forename: C
authors.initials: C
authors.lastname: Petrakis
authors.forename: P
authors.initials: P
authors.lastname: Kefalas
mesh_terms: D000975:Antioxidants / Q000032:analysis; D001205:Ascorbic Acid / Q000032:analysis; D015764:Bepridil / Q000031:analogs & derivatives* / Q000737:chemistry*; D001713:Biphenyl Compounds; D002839:Chromans / Q000032:analysis; D003035:Cobalt / Q000737:chemistry*; D004492:Edetic Acid / Q000737:chemistry*; D016166:Free Radical Scavengers / Q000032:analysis*; D066298:In Vitro Techniques; D007202:Indicators and Reagents / Q000737:chemistry*; D008163:Luminescent Measurements; D008165:Luminol / Q000737:chemistry*; D010851:Picrates; D011794:Quercetin / Q000032:analysis
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D002839:Chromans; D016166:Free Radical Scavengers; D007202:Indicators and Reagents; D010851:Picrates; D003035:Cobalt; D008165:Luminol; D015764:Bepridil; D004492:Edetic Acid; D011794:Quercetin; C004931:1,1-diphenyl-2-picrylhydrazyl; D001205:Ascorbic Acid; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid