Rme-1 regulates the distribution and function of the endocytic recycling compartment in mammalian cells.
RME-1 is an Eps15-homology (EH)-domain protein that was identified in a genetic screen for endocytosis genes in Caenorhabditis elegans. When expressed in a CHO cell line, the worm RME-1 protein and a mouse homologue are both associated with the endocytic recycling compartment. Here we show that expression of a dominant-negative construct with a point mutation near the EH domain results in redistribution of the endocytic recycling compartment and slowing down of transferrin receptor recycling. The delivery of a TGN38 chimaeric protein to the trans-Golgi network is also slowed down. The function of Rme-1 in endocytic recycling is evolutionarily conserved in metazoans as shown by the protein's properties in C. elegans.
Lin
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SX
Department of Biochemistry, Weill Medical College of Cornell University, 1300 York Avenue, New York, New York 10021, USA.
Grant
B
B
Hirsh
D
D
Maxfield
F R
FR
D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D016466:CHO Cells; D029742:Caenorhabditis elegans Proteins; D002135:Calcium-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D002451:Cell Compartmentation / Q000502:physiology*; D006224:Cricetinae; D045683:Furin; D006023:Glycoproteins; D047908:Intracellular Signaling Peptides and Proteins; D008562:Membrane Glycoproteins / Q000378:metabolism; D008565:Membrane Proteins; D051379:Mice; D009154:Mutation; D010750:Phosphoproteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D017434:Protein Structure, Tertiary; D013381:Subtilisins / Q000378:metabolism; D014168:Transferrin / Q000378:metabolism; D022161:Transport Vesicles / Q000502:physiology*
D048868:Adaptor Proteins, Signal Transducing; D029742:Caenorhabditis elegans Proteins; D002135:Calcium-Binding Proteins; C082729:Eps15 protein, mouse; D006023:Glycoproteins; D047908:Intracellular Signaling Peptides and Proteins; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D010750:Phosphoproteins; C486205:RME-1 protein, C elegans; C513047:Tgoln1 protein, mouse; C513046:Tgoln2 protein, mouse; D014168:Transferrin; D013381:Subtilisins; D045683:Furin