A protein with characteristics of factor H is present on rodent platelets and functions as the immune adherence receptor.
Complement-coated particles interact with specific immune adherence receptors (IAR). In primates, this function is served by complement receptor 1 (CR1) on erythrocytes. In contrast, rodent platelets bear IAR distinct from CR1, the identity of which was studied here. A 150-kDa C3b-binding protein was isolated from rat platelets, which had immunochemical and biochemical identity to plasma factor H. Immunofluorescence microscopy and flow cytometry demonstrated that factor H was present on the surface of rat and mouse platelets, which could be removed by treatment with neuraminidase. Sheep erythrocytes bearing C3b underwent immune adherence with rat and mouse platelets, which was blocked with anti-factor H F(ab')(2) antibodies, but not with antibodies binding to the complement regulator, Crry, on the platelet surface. By reverse transcription-polymerase chain reaction using rat platelet RNA and primers designed from mouse factor H, a 472-base pair product was generated that was identical in sequence to that produced from rat liver RNA. The translated protein product was 85% similar to mouse liver factor H. The 3'-nucleotide sequence from platelets predicted a soluble factor H protein. By Northern analysis, liver and platelets had identically sized factor H mRNA. Thus, rat and mouse platelets have a membrane protein with characteristics of factor H that is linked via sialic acid residues and functions as the IAR. Whether platelet factor H is acquired by passive adsorption from sera and/or is produced by platelets remains to be determined.
Alexander
J J
JJ
Section of Nephrology, Department of Medicine, The University of Chicago, Chicago, Illinois 60637, USA. jealexand@medicine.uchicago.edu
Hack
B K
BK
Cunningham
P N
PN
Quigg
R J
RJ
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001792:Blood Platelets / Q000378:metabolism*; D017242:Complement Factor H / Q000378:metabolism* / Q000502:physiology; D017931:DNA Primers; D051379:Mice; D008969:Molecular Sequence Data; D051381:Rats; D011971:Receptors, Immunologic / Q000502:physiology*; D017386:Sequence Homology, Amino Acid
D017931:DNA Primers; D011971:Receptors, Immunologic; D017242:Complement Factor H