Toxicity and mutagenesis of chrysotile asbestos to Agrobacterium radiobacter.
The mutation of Agrobacterium radiobacter cells exposed to chrysotile asbestos was examined by the random amplified polymorphic DNA (RAPD) method. Approximately 1.4 kbp of DNA in A. radiobacter, which was not amplified strongly in the cells that were not exposed to asbestos, was amplified in the cells that were exposed to asbestos. Mutation in genomic DNA of A. radiobacter was found to be induced by asbestos. Specific DNA that was amplified by asbestos present in PCR products and that which exists latently in genomic DNA were cloned, and these sequences were then determined and compared. It was shown that one of the mutations by the asbestos in the A. radiobacter occurred only in the primer annealed region and was a point mutation or deletion.
Yoshida
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N
Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, Miyazaki University, 1-1 Gakuen Kibanadai-Nishi, Miyazaki-shi 889-2192, Japan. a04109u@cc.miyazaki-u.ac.jp
Naka
T
T
Sengoku
T
T
Ogawa
K
K
D017632:Asbestos, Serpentine / Q000633:toxicity*; D001483:Base Sequence; D003001:Cloning, Molecular; D004252:DNA Mutational Analysis; D004269:DNA, Bacterial; D018095:Germ-Line Mutation; D008969:Molecular Sequence Data; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D019105:Random Amplified Polymorphic DNA Technique; D012231:Rhizobium / Q000187:drug effects*; D017384:Sequence Deletion
D017632:Asbestos, Serpentine; D004269:DNA, Bacterial; D009153:Mutagens