Temperature stability of vaginal specimens for Chlamydia trachomatis detection by Amplicor polymerase chain reaction assay.
Vaginal introital specimens were collected from 17 women - 10 positive and 7 negative for Chlamydia trachomatis, and kept in Amplicor collection medium at ambient temperature. Aliquots were removed at intervals for up to 34 days and frozen at -80 degrees C. Samples were thawed and assayed for C. trachomatis by polymerase chain reaction (PCR). The results of all specimens remained unchanged over this time interval.
Witkin
S S
SS
Division of Immunology, Department of Obstetrics and Gynecology, Weill Medical College of Cornell University, New York, New York 10021, USA. switkin@mail.med.cornell.edu
Tolbert
V
V
Sharma
G
G
Polaneczky
M
M
D002690:Chlamydia Infections / Q000382:microbiology*; D002692:Chlamydia trachomatis / Q000235:genetics / Q000302:isolation & purification*; D005260:Female; D006801:Humans; D016133:Polymerase Chain Reaction; D013048:Specimen Handling; D013696:Temperature; D014623:Vaginal Diseases / Q000382:microbiology*; D014626:Vaginal Smears