title: Angiotensinogen and endothelial nitric oxide synthase gene polymorphisms among Hispanic patients with preeclampsia.
abstract: OBJECTIVE
  We sought to establish an association between preeclampsia and the methionine to threonine polymorphism at amino acid residue 235 (Met235Thr) in angiotensinogen in a Hispanic population. We looked for a relationship between this allele and the allele in the endothelial nitric oxide synthase gene (NOS3) that produces the A form (NOS3*A) with respect to preeclampsia.
  
  
  METHODS
  Clinical data were collected from 87 patients with preeclampsia and 53 control subjects. Patients and controls were genotyped for the angiotensinogen polymorphism allele (AGT*T) and the NOS3*A polymorphism. We then compared patients with preeclampsia and control subjects and investigated disease severity within the preeclampsia group as a function of genotype.
  
  
  RESULTS
  The AGT*T allelic frequencies among patients with preeclampsia and control subjects were 0.72 and 0.70, respectively (P =.84). The blood pressure of patients with an AGT*T allele who also carried a NOS3*A allele was higher at earlier gestational ages (r = -0.052; P =.02). Analysis suggested that the systolic blood pressure differences were due to gestational age effects and the presence of a NOS3*A allele (P <.10).
  
  
  CONCLUSIONS
  The AGT*T allele was not associated with the development of preeclampsia. Independently of the presence of an AGT*T allele, the NOS3*A allele was associated with a higher blood pressure at an earlier gestational age.
authors.affiliation: Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA.
authors.forename: M T
authors.initials: MT
authors.lastname: Bashford
authors.forename: L A
authors.initials: LA
authors.lastname: Hefler
authors.forename: T W
authors.initials: TW
authors.lastname: Vertrees
authors.forename: B B
authors.initials: BB
authors.lastname: Roa
authors.forename: A R
authors.initials: AR
authors.lastname: Gregg
mesh_terms: D000328:Adult; D000483:Alleles; D000808:Angiotensinogen / Q000235:genetics*; D001794:Blood Pressure; D005260:Female; D005787:Gene Frequency; D005865:Gestational Age; D006630:Hispanic or Latino / Q000706:statistics & numerical data*; D006801:Humans; D019001:Nitric Oxide Synthase / Q000235:genetics*; D052250:Nitric Oxide Synthase Type III; D011110:Polymorphism, Genetic; D011225:Pre-Eclampsia / Q000235:genetics* / Q000503:physiopathology; D011247:Pregnancy; D012016:Reference Values
chemical_list: D000808:Angiotensinogen; C496314:NOS3 protein, human; D019001:Nitric Oxide Synthase; D052250:Nitric Oxide Synthase Type III