D-galactose-specific sea urchin lectin sugar-specifically inhibited histamine release induced by datura stramonium agglutinin: differences between sugar-specific effects of sea urchin lectin and those of D-galactose- or L-fucose-specific plant lectins.
A new sea urchin lectin from Toxopneustes pileolus, is D(+)galactose (Gal)-, D(+)fucose (Fuc)-specific. Incubation of rat peritoneal mast cells with the lectin in the presence of 0.3 mM CaCl2 for 10 min significantly and dose-dependently inhibited the histamine release induced by N-acetyl glucosamine (GlcNAc)-specific Datura stramonium agglutinin (DSA), an activator of the Gi-protein-dependent pathway in mast cells. This inhibition by the sea urchin lectin was sugar-specifically reversed in the presence of D(+)Gal or D(+)Fuc but not L(-)Fuc. The sea urchin lectin had no effect on the histamine release induced by compound 48/80, slightly inhibited the histamine release induced by substance P and mastoparan, and slightly enhanced the histamine release induced by melittin, but these effects were not dose-dependent. Compound 48/80, substance P, mastoparan and melittin are mast cell activators without sugar residues. It is suggested that the lectin binds to D(+)Gal residues of DSA to interfere with mast cell activation induced by DSA, a glycoprotein with arabinose and Gal residues. The effects of plant lectins with affinity to D(+)Gal, N-acetyl galactosamine and/or sialic acid and L(-)Fuc on the histamine release induced by DSA, compound 48/80 and substance P were also examined.
Suzuki-Nishimura
T
T
Department of Pharmacology, Meiji Pharmaceutical University, Tokyo, Japan.
Nakagawa
H
H
Uchida
M K
MK
D000373:Agglutinins / Q000494:pharmacology*; D000818:Animals; D005690:Galactose / Q000494:pharmacology*; D006636:Histamine Release / Q000187:drug effects* / Q000502:physiology; D037102:Lectins / Q000302:isolation & purification / Q000494:pharmacology*; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism; D037121:Plant Lectins; D051381:Rats; D017207:Rats, Sprague-Dawley; D012617:Sea Urchins / Q000378:metabolism*; D013373:Substance P / Q000494:pharmacology; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
D000373:Agglutinins; C041384:Datura stramonium lectin; D037102:Lectins; D037121:Plant Lectins; C027112:fucose-binding lectin; D013373:Substance P; D003189:p-Methoxy-N-methylphenethylamine; D005690:Galactose