The production and evaluation of antibodies for enzyme immunoassay of AZTTP.
We describe the development of the first enzyme immunoassay for quantifying AZTTP that does not use of radioactive labeling. Anti-AZTTP antibodies were raised in rabbits by immunizing with an AZTTP-kelhoyle limpet hemocyanin (KLH) conjugate. Competitive immunoassays indicated a nanomolar sensitivity to AZTTP. One of the antisera produced was specific for AZTTP.
Akeb
F
F
Laboratoire de Chimie Bio-Organique, ESA CNRS 6001, Faculté des Sciences, Université de Nice-Sophia Antipolis, France.
Creminon
C
C
Grassi
J
J
Guedj
R
R
Duval
D
D
D000110:Acetylcholinesterase / Q000737:chemistry; D000818:Animals; D019380:Anti-HIV Agents / Q000032:analysis* / Q000737:chemistry / Q000276:immunology*; D000906:Antibodies / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism; D002138:Calibration; D054306:Dideoxynucleotides; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006433:Hemocyanins / Q000737:chemistry / Q000276:immunology; D015394:Molecular Structure; D011817:Rabbits; D012680:Sensitivity and Specificity; D013942:Thymine Nucleotides / Q000032:analysis* / Q000737:chemistry / Q000276:immunology*; D015215:Zidovudine / Q000031:analogs & derivatives* / Q000032:analysis* / Q000737:chemistry / Q000276:immunology*
D019380:Anti-HIV Agents; D000906:Antibodies; D054306:Dideoxynucleotides; D013942:Thymine Nucleotides; D015215:Zidovudine; C052873:zidovudine triphosphate; D006433:Hemocyanins; D000110:Acetylcholinesterase; C032808:keyhole-limpet hemocyanin