Production and separation of alpha-agarase from Altermonas agarlyticus strain GJ1B.
This paper presents results on the production of alpha-agarase by a fermentation process and its separation using membrane microfiltration (MF). Optimization of fermentation conditions for alpha-agarase production using Altermonas agarlyticus grown on medium containing agar as a carbon source was done in batch, fed-batch and continuous fermentations. Continuous culture at a dilution rate of 0.03 h(-1) appeared to be best suited for production of alpha-agarase by this organism. At 0.03 h(-1) dilution rate, enzyme activity was 0.9 U/ml. Clarification of broth was done using a hollow-fibre microfiltration membrane. The influence of hydrodynamic parameters on permeate flux and enzyme activity was studied. The best performance was obtained with prefiltered fermentation broth. A stable permeate flux of about 250-270 ml/min.m2 and an enzyme retention rate between 0% and 25% was obtained at temperatures between 6 degrees C and 22 degrees C, transmembrane pressure of 100 mm Hg and fluid cross-flow velocity of 4 x 10(-2) m/s. From the experiments on concentration of fermentation broth, the best compromise between enzyme activity transmission and permeate flux was obtained at a concentration factor of 2.
Hassairi
I
I
Centre de Biotechnologie de Sfax, Tunisia.
Ben Amar
R
R
Nonus
M
M
Gupta
B B
BB
D020591:Alteromonas / Q000201:enzymology*; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000302:isolation & purification*; D003470:Culture Media / Q000737:chemistry; D005285:Fermentation; D006026:Glycoside Hydrolases / Q000096:biosynthesis* / Q000302:isolation & purification*; D013696:Temperature
D001426:Bacterial Proteins; D003470:Culture Media; D006026:Glycoside Hydrolases; C019624:agarase