Stochastic modeling of affinity adsorption.
A stochastic model is described that allows surface proximity and packing effects to be incorporated into predictions of adsorption kinetics and equilibrium of affinity adsorption. Equilibrium predictions show that, depending on conditions chosen, the results obtained for equilibrium conditions can exhibit either a Freundlich- or a Langmuir-type relationship. Under conditions of surface density imposed adsorption constraints, the time taken for equilibrium to be reached increases as the "off" constant is decreased. This suggests that for resins having a high immobilized ligand density binding kinetics may be more highly limited by the "off" constant than by mass transfer limitations.
Hubble
J
J
Department of Chemical Engineering, University of Bath, Claverton Down, Bath, BA2 7AY, U.K. j.hubble@bath.ac.uk
D000327:Adsorption; D001665:Binding Sites; D008024:Ligands; D008962:Models, Theoretical; D011506:Proteins / Q000737:chemistry / Q000378:metabolism; D013269:Stochastic Processes
D008024:Ligands; D011506:Proteins