Degradation of bisphenol A by purified laccase from Trametes villosa.
Degradation of bisphenol A (BPA), an endocrine-disrupting chemical, was studied with a purified laccase from the basidiomycete Trametes villosa. SDS--polyacrylamide gel electrophoresis of the purified laccase gave one single band with a mobility corresponding to MW 65 kDa. The absorption spectrum showed the characteristics of a blue copper protein with a maximum peak at 600 nm. HPLC analysis revealed that 2.2 micromol BPA were degraded by incubation with 1.5 units of the purified laccase in a total volume of 1.0 ml at pH 6.0 and 60 degrees C for 3 h. The enzyme reaction proceeded rapidly without requirement of mediators for the electron transfer. Isolation and identification of several reaction products are in progress, in which one product was identified as 4-isopropenylphenol by a gas chromatography--mass spectrophotometer.
Fukuda
T
T
Applied Chemistry and Biotechnology, Faculty of Engineering, Fukui University, 9-1, Bunkyou 3-Chome, Fukui-shi 910-8507, Japan.
Uchida
H
H
Takashima
Y
Y
Uwajima
T
T
Kawabata
T
T
Suzuki
M
M
D001559:Benzhydryl Compounds; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000302:isolation & purification / Q000494:pharmacology; D042845:Laccase; D013058:Mass Spectrometry; D008667:Metalloproteins / Q000302:isolation & purification / Q000494:pharmacology; D015591:Microspectrophotometry; D010088:Oxidoreductases / Q000302:isolation & purification / Q000494:pharmacology*; D010636:Phenols / Q000378:metabolism*; D020072:Polyporales / Q000201:enzymology*; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D005656:Fungal Proteins; D042845:Laccase; D008667:Metalloproteins; D010088:Oxidoreductases; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds