In vivo nuclear delivery of oligonucleotides via hybridizing bifunctional peptides.
Linking proteins directly to nucleic acids has been a complex task. By hybridizing a bifunctional peptide nucleic acid (PNA) consisting of a nucleic acid binding moiety and a nuclear localization signal (NLS) we have previously demonstrated that it is possible to link protein functions directly to nucleic acids containing a PNA target site. By hybridizing fluorescently labeled oligonucleotides to PNA-NLS molecules and subsequently transfecting different organs in vivo we demonstrate an active nuclear translocation of the PNA-NLS/oligonucleotide complex in different mouse organs.
Brandén
L J
LJ
Department of Biosciences, Karolinska Institutet, NOVUM, and Department of Immunology, Microbiology, Pathology and Infectious Diseases, Huddinge University Hospital, Huddinge, Sweden.
Christensson
B
B
Smith
C I
CI
D000818:Animals; D002467:Cell Nucleus; D051379:Mice; D008808:Mice, Inbred CBA; D019913:Nuclear Localization Signals / Q000235:genetics; D009841:Oligonucleotides / Q000235:genetics*; D020135:Peptide Nucleic Acids / Q000235:genetics*; D014162:Transfection / Q000379:methods*; D014178:Translocation, Genetic
D019913:Nuclear Localization Signals; D009841:Oligonucleotides; D020135:Peptide Nucleic Acids