title: Distribution of resistance genes tet(M), aph3'-III, catpC194 and the integrase gene of Tn1545 in clinical Streptococcus pneumoniae harbouring erm(B) and mef(A) genes in Spain.
abstract: The most prevalent macrolide resistance phenotype and genotype among pneumococcal isolates was the cMLSB phenotype [erm(B) or erm(B)/mef(A)] (91.3%). We studied the distribution of other resistance genes, tet(M), catpC194, aph3'-III, in these strains, seeing evolution at work in that some strains carried different combinations of resistance determinants. The most prevalent patterns associated with resistance to erythromycin [erm(B)] were resistance to tetracycline [tet(M)] and chloramphenicol (catpC194) (48.2%) or resistance to tetracycline [tet(M)] alone (42.2%). In our isolates of Streptococcus pneumoniae there was a strong association of the erm(B) and tet(M) genes with Tn1545-related elements.
authors.affiliation: Department of Microbiology, University Hospital 'Lozano Blesa', San Juan Bosco 15, 50009 Zaragoza, Spain. c.seral.000@recol.es
authors.forename: C
authors.initials: C
authors.lastname: Seral
authors.forename: F J
authors.initials: FJ
authors.lastname: Castillo
authors.forename: M C
authors.initials: MC
authors.lastname: Rubio-Calvo
authors.forename: A
authors.initials: A
authors.lastname: Fenoll
authors.forename: C
authors.initials: C
authors.lastname: García
authors.forename: R
authors.initials: R
authors.lastname: Gómez-Lus
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics*; D002702:Chloramphenicol Resistance / Q000235:genetics; D003227:Conjugation, Genetic / Q000235:genetics; D004251:DNA Transposable Elements / Q000235:genetics*; D004352:Drug Resistance, Microbial / Q000235:genetics; D006801:Humans; D019426:Integrases / Q000235:genetics*; D019868:Kanamycin Kinase / Q000235:genetics*; D018942:Macrolides; D008565:Membrane Proteins / Q000378:metabolism; D008780:Methyltransferases / Q000378:metabolism; D008826:Microbial Sensitivity Tests; D013030:Spain; D013296:Streptococcus pneumoniae / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D018942:Macrolides; C117866:MefA protein, Streptococcus; D008565:Membrane Proteins; C083138:Tet M resistance protein, Bacteria; D008780:Methyltransferases; C035181:rRNA (adenosine-O-2'-)methyltransferase; D019868:Kanamycin Kinase; D019426:Integrases