title: Age-related alterations of cardiac tissue microstructure and material properties in Fischer 344 rats.
abstract: The cardiac aging process is accompanied by global mechanical dysfunction that reflects increased myocardial stiffness. Accordingly, age-related changes in microscopic material properties of myocardium were delineated with high-frequency ultrasound (US) (30 to 44 MHz) tissue characterization methods for aging Fischer 344 rats at 6 (adult), 18 (aged), and 24 (senescent) months of age. The excised lateral wall of the left ventricle of rats (n = 10 per group) was insonified with a 50-MHz acoustic microscope for determination of integrated backscatter, backscatter coefficient and attenuation coefficient. Histological and biochemical analyses for collagen content and cardiac myocyte diameter were performed. Collagen concentration increased progressively with age, with the greatest increments occurring from 6 to 18 months (38.0 +/- 6.3 to 53.0 +/- 7.1 mg/g dry wt), and leveling off at 24 months (60.0 +/- 7.4 mg/g dry wt). Tissue microscopic material properties also changed progressively from 6 to 24 months of age, as determined by US methods: integrated backscatter increased (-44.7 +/- 1.8 vs. -40.8 +/- 1.9 dB, p < 0.05), attenuation increased (47.1 +/- 5.9 to 65.3 +/- 7.8 dB/cm, p < 0.05), and the backscatter coefficient increased (0.73 +/- 0.16 x 10(-5) to 3.76 +/- 1.6 x 10(-5) cm(-1), p < 0.05), from 6 to 24 months of age in each case. Age-related alterations in indices of cardiac microscopic material properties were closely correlated with the changes in cardiac microstructure. Ultrasonic tissue characterization may prove to be a sensitive tool to monitor changes in the cardiac microstructure, such as increased collagen deposition, that occur within age-related diastolic dysfunction.
authors.affiliation: Cardiovascular Division, Barnes-Jewish Hospital at Washington University School of Medicine, St. Louis, MO 63110, USA.
authors.forename: C T
authors.initials: CT
authors.lastname: Nguyen
authors.forename: C S
authors.initials: CS
authors.lastname: Hall
authors.forename: M J
authors.initials: MJ
authors.lastname: Scott
authors.forename: Q
authors.initials: Q
authors.lastname: Zhu
authors.forename: J
authors.initials: J
authors.lastname: Marsh
authors.forename: S A
authors.initials: SA
authors.lastname: Wickline
mesh_terms: D000375:Aging / Q000473:pathology* / Q000502:physiology*; D000818:Animals; D004195:Disease Models, Animal; D004452:Echocardiography; D006333:Heart Failure / Q000000981:diagnostic imaging* / Q000473:pathology / Q000503:physiopathology; D008297:Male; D009206:Myocardium / Q000166:cytology*; D011846:Radio Waves; D051381:Rats; D011916:Rats, Inbred F344; D017207:Rats, Sprague-Dawley