Functional restoration of the bursa of Fabricius following in ovo infectious bursal disease vaccination.
The primary role of the avian bursa of Fabricius is to provide an essential microenvironment for B-lymphocytes to diversify their immunoglobulin genes by gene hyperconversion. Infectious bursal disease (IBD) vaccination using intermediate plus vaccine strains can temporarily deplete the bursal follicles and interrupt the normal B-cell development, which is generally followed by B-cell repopulation and histological regeneration. To find evidence that functional restoration of the bursa of Fabricius occurs in addition to the histological regeneration, we have analysed the chB1 gene expression, which indicates active bursal B-lymphocytes, and also the surface expression of a carbohydrate structure Lewis(x), a marker which identifies those bursal B-lymphocytes that are undergoing gene hyperconversion. In ovo vaccination with an immune complex vaccine (IBDV-BDA) caused transient bursal destruction in both the SPF and the maternally protected broiler groups with differences evident in the starting time, the severity and the duration of the effect. After the depletion phase, signs of histological regeneration appeared together with chB1- and Lewis(x) expression indicating that B-lymphocytes were functionally active and the bursa of Fabricius was serving again as an efficient primary lymphoid organ providing an appropriate microenvironment for B-cell development.
Iván
J
J
Veterinary Medical Research Institute, Hungarian Academy of Sciences, P.O. Box 18, H-1581, Budapest, Hungary. judit@novell.vmri.hu
Nagy
N
N
Magyar
A
A
Kacskovics
I
I
Mészáros
J
J
D000818:Animals; D000936:Antigen-Antibody Complex / Q000276:immunology; D001426:Bacterial Proteins; D018175:Birnaviridae Infections / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D015152:Blotting, Northern / Q000662:veterinary; D002060:Bursa of Fabricius / Q000276:immunology* / Q000502:physiology; D002352:Carrier Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification; D002642:Chick Embryo; D002645:Chickens / Q000276:immunology*; D007113:Immunity, Innate / Q000276:immunology; D007150:Immunohistochemistry; D007243:Infectious bursal disease virus / Q000276:immunology*; D047908:Intracellular Signaling Peptides and Proteins; D016256:Lewis X Antigen / Q000096:biosynthesis / Q000097:blood / Q000235:genetics; D009500:Neutralization Tests / Q000662:veterinary; D011201:Poultry Diseases / Q000276:immunology* / Q000821:virology; D012367:RNA, Viral / Q000096:biosynthesis / Q000235:genetics; D012038:Regeneration / Q000276:immunology; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D013047:Specific Pathogen-Free Organisms; D014158:Transcription, Genetic; D014611:Vaccination / Q000662:veterinary; D014765:Viral Vaccines / Q000009:adverse effects / Q000276:immunology*
D000936:Antigen-Antibody Complex; D001426:Bacterial Proteins; C091405:CHB1 protein, Streptomyces; D002352:Carrier Proteins; D047908:Intracellular Signaling Peptides and Proteins; D016256:Lewis X Antigen; D012367:RNA, Viral; D014765:Viral Vaccines