title: Application of capillary electrochromatography using macroporous polyacrylamide columns for the analysis of lignans from seeds of Schisandra chinensis.
abstract: Capillary electrochromatography (CEC) using polymer-based monolithic stationary phase has been developed as a promising method for the determination of lignans of Schisandra chinensis. The columns were prepared by in situ copolymerisation of acrylamide, N,N'-methylenebisacrylamide, vinylsulfonic acid and lauryl acrylate in presence of poly(ethylene glycol) as a porogenic agent. The columns [33 cm (24.5 cm effective length) x 75 microm I.D.] were successfully used to analyse and quantify the major lignans in extract of the seeds of Schisandra chinensis. Good separations were achieved in less than 35 min. The calibration graphs were linear in the range 0.025-1.0 mg/ml of given lignan with correlation coefficients between 0.9951 and 0.9996. The inter-day reproducibility of the peak area were below 3.9% and the inter-day reproducibility of the migration time were below 4.2%. The results of quantitative CEC analyses were compared with those obtained by reversed-phase HPLC, the levels of schizandrin, gomisin A, gomisin N and wuweizisu C determined by CEC were in a good agreement with those determined by HPLC.
authors.affiliation: Department of Biochemistry, Faculty of Science, Masaryk University, Brno, Czech Republic.
authors.forename: L
authors.initials: L
authors.lastname: Kvasnicková
authors.forename: Z
authors.initials: Z
authors.lastname: Glatz
authors.forename: H
authors.initials: H
authors.lastname: Sterbová
authors.forename: V
authors.initials: V
authors.lastname: Kahle
authors.forename: J
authors.initials: J
authors.lastname: Slanina
authors.forename: P
authors.initials: P
authors.lastname: Musil
mesh_terms: D000180:Acrylic Resins; D002851:Chromatography, High Pressure Liquid; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000295:instrumentation / Q000379:methods*; D017705:Lignans / Q000032:analysis*; D019684:Magnoliopsida / Q000196:embryology*; D015203:Reproducibility of Results; D012639:Seeds / Q000737:chemistry*; D000099166:Polyacrylamides
chemical_list: D000180:Acrylic Resins; D017705:Lignans; D000099166:Polyacrylamides