Transcriptional termination factors for RNA polymerase II in yeast.
The molecular connections between mRNA 3' end processing and transcriptional termination have been investigated in S. pombe using a genetic screen. By this approach, we have identified a RNAP II termination domain in the well-defined cleavage polyadenylation factor called CstF-64 in metazoans and Rna15p in S. cerevisiae. Furthermore, this C-terminal domain interacts with Res2, previously identified as a component of the G1/S transition-specific transcription factor MBF. Deletion of res2 in both fission and budding yeast results in a defect in 3' end formation. This raises the possibility that RNAP II transcriptional termination may in some situations be integrated with cell cycle events.
Aranda
A
A
Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, United Kingdom.
Proudfoot
N
N
D020413:3' Untranslated Regions / Q000235:genetics; D000595:Amino Acid Sequence; D002453:Cell Cycle / Q000235:genetics; D004268:DNA-Binding Proteins; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D017353:Gene Deletion; D008969:Molecular Sequence Data; D010454:Peptide Termination Factors / Q000235:genetics*; D012319:RNA Polymerase II / Q000235:genetics*; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D012568:Schizosaccharomyces / Q000235:genetics*; D029702:Schizosaccharomyces pombe Proteins; D016415:Sequence Alignment; D013728:Terminator Regions, Genetic; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic; D039221:mRNA Cleavage and Polyadenylation Factors
D020413:3' Untranslated Regions; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D010454:Peptide Termination Factors; D016601:RNA-Binding Proteins; D029702:Schizosaccharomyces pombe Proteins; D014157:Transcription Factors; D039221:mRNA Cleavage and Polyadenylation Factors; C087021:res2 protein, S pombe; D012319:RNA Polymerase II