Pronounced effect of minor changes in body temperature on ischemia and reperfusion injury in rat liver.
This study examined the effects of 1 degrees C hypo- or hyperthermia on in vivo liver ischemia and reperfusion (I/R) injury in 15 fasted male Wistar rats. Rats were ventilated, and rectal temperature was maintained at 36, 37 (normothermic), or 38 degrees C. In all rats, 70% liver ischemia was induced by clamping the afferent vessels to the median and left lateral lobes for 60 min, and reperfusion was allowed for 90 min. Changes in plasma aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alpha-glutathione S-transferase (alpha-GST) levels were measured, hemodynamics and bile secretion were monitored, and arterial blood-gas analysis was performed. All ventilated rats showed a normal pH, arterial PCO(2), and arterial PO(2). AST, ALT, and alpha-GST levels were significantly higher in the 38 degrees C group when compared with the 36 and 37 degrees C groups after ischemia. No differences in bile secretion were found between all groups. Histopathological alterations were in agreement with AST, ALT, and alpha-GST levels in plasma. We conclude that a decrease of only 1 degrees C in body temperature significantly attenuates liver I/R injury, whereas an increase of 1 degrees C significantly increases liver I/R injury.
Heijnen
B H
BH
Surgical Laboratory, Academic Medical Center, 1105 AZ Amsterdam, The Netherlands. b.h.heijnen@amc.uva.nl
van Veen
S Q
SQ
Straatsburg
I H
IH
van Gulik
T M
TM
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001831:Body Temperature / Q000502:physiology*; D005740:Gases / Q000097:blood; D005982:Glutathione Transferase / Q000097:blood; D006439:Hemodynamics; D007511:Ischemia / Q000473:pathology* / Q000503:physiopathology*; D008099:Liver / Q000473:pathology; D008102:Liver Circulation; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000473:pathology* / Q000503:physiopathology*
D005740:Gases; D005982:Glutathione Transferase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase