Opposing pharmacological actions of cepharanthin on lipopolysaccharide-induced histidine decarboxylase activity in mice spleens.
A biscoclaurin alkaloid preparation, cepharanthin (Ceph), is reported to have opposing pharmacological effects, enhancement or depression, on several cells and tissues, although detailed mechanisms remain unclear. Previously, we reported that Ceph enhanced lipopolysaccharide (LPS)-induced histidine decarboxylase (HDC) activity in mice spleens by consecutive pre-administration. In this study, we examined the pharmacological effects on HDC activity of a single Ceph pre-administration to test the influence of the administration method. Consequently, HDC activities were decreased by a single administration 15 minutes before LPS challenge in ddY and ICR mice spleens. Moreover, to further examine this suppressing effect, we employed genetically mast cell-deficient WBB6F1 W/Wv (W/Wv) mice to avoid the influence of mast cells. In W/Wv mice, HDC activity was enhanced, but not in the congenic WBB6F1 +/+ mice. These findings suggest that mast cells influence the depressant effect on HDC activity by a Ceph single administration in mast cell sufficient mice.
Sogawa
N
N
Department of Dental Pharmacology, Okayama University of Dental School, Japan. sogawa@dent.okayama-u.ac.jp
Sogawa
C A
CA
Abuku
E I
EI
Inoue
T
T
Oda
N
N
Kishi
K
K
Furuta
H
H
D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D044182:Benzylisoquinolines; D015550:Cell Degranulation / Q000187:drug effects / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D004926:Escherichia coli / Q000276:immunology; D006640:Histidine Decarboxylase / Q000096:biosynthesis*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000473:pathology / Q000502:physiology; D051379:Mice; D008813:Mice, Inbred ICR / Q000235:genetics; D018345:Mice, Knockout; D012867:Skin / Q000187:drug effects / Q000473:pathology; D013154:Spleen / Q000187:drug effects* / Q000201:enzymology; D052117:Benzodioxoles
D000470:Alkaloids; D000894:Anti-Inflammatory Agents, Non-Steroidal; D044182:Benzylisoquinolines; D006640:Histidine Decarboxylase; D008070:Lipopolysaccharides; C006947:cepharanthine; D052117:Benzodioxoles