Aromatase expression and activity in male and female cultured rat hypothalamic neurons: effect of androgens.
Aromatase is possibly involved in male brain sexual differentiation. Aim of these experiments was to evaluate the role of testosterone (T) and of DHT, in the regulation of aromatase expression and activity. The experiments were done utilizing rat primary cultures of hypothalamic neurons from 16-day old embryos sex-screened by SRY gene. Aromatase expression was assessed semiquantitatively by RT-PCR using a neuronal marker (MAP2c) as coamplification product; enzymatic activity was estimated by the 3H(2)O method. The results indicate that (1) cultured neurons possess a functional aromatase, which increases significantly during a 5-days culture period; (2) neurons from males possess a higher expression and activity of the enzyme than females; (3) androgens negatively control expression/activity of aromatase in males, DHT is more active than T; (4) on the contrary, in females T produces a small stimulation of aromatase expression, but not of activity (DHT has produced inconsistent results). The results obtained in this model indicate that T does not stimulate aromatase; therefore, it is not responsible for triggering the perinatal enzymatic peak, nor for the sexual dimorphic aromatase expression. A model is proposed in which DHT might induce, at least in males, the descending phase of the aromatase peak.
Negri-Cesi
P
P
Department of Endocrinology, University of Milano, via Balzaretti 9, 20133, Milan, Italy. paola.negricesi@unimi.it
Colciago
A
A
Motta
M
M
Martini
L
L
Celotti
F
F
D000818:Animals; D001141:Aromatase / Q000235:genetics* / Q000378:metabolism*; D001483:Base Sequence; D002478:Cells, Cultured; D017931:DNA Primers / Q000235:genetics; D013196:Dihydrotestosterone / Q000494:pharmacology; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D015870:Gene Expression; D007031:Hypothalamus / Q000187:drug effects / Q000196:embryology / Q000201:enzymology*; D008297:Male; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism; D008954:Models, Biological; D009474:Neurons / Q000187:drug effects / Q000201:enzymology; D011374:Progesterone / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012733:Sex Differentiation; D013739:Testosterone / Q000494:pharmacology
D017931:DNA Primers; C489787:MAP2 protein, rat; D008869:Microtubule-Associated Proteins; D012333:RNA, Messenger; D013196:Dihydrotestosterone; D013739:Testosterone; D011374:Progesterone; D004958:Estradiol; D001141:Aromatase