HSP 47 and collagen mRNA expression in L929 cells adhered to lipid films.
L929 cell adhesion on various lipid films prepared by Langmuir-Blodgett methods (LB method) were studied. L929 cells adhered to every lipid film similar to tissue culture poly(styrene) (TCPS). The mRNA expression of both collagen and HSP47 in adherent cells were evaluated by the RT-PCR method. mRNA expression of collagen was not altered during adhesion and proliferation duration, while HSP47 mRNA expression changed depending on culture time. L929 cells adhered to L-alpha-dipalmitoylphosphatidylcholine (DPPC)-films showed little HSP47 mRNA expression. It was suggested that DPPC films regulate L929 cell function via unique serum protein adsorption.
Kato
S
S
Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Kagoshima University, Korimoto, Japan.
Matsuyama
T
T
Serizawa
T
T
Kishida
A
A
Akashi
M
M
D015060:1,2-Dipalmitoylphosphatidylcholine; D000818:Animals; D001672:Biocompatible Materials; D002448:Cell Adhesion; D002460:Cell Line; D003094:Collagen / Q000235:genetics*; D016895:Culture Media, Serum-Free; D015870:Gene Expression; D050958:HSP47 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000235:genetics*; D008055:Lipids; D008422:Materials Testing; D051379:Mice; D011137:Polystyrenes; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism*
D001672:Biocompatible Materials; D016895:Culture Media, Serum-Free; D050958:HSP47 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D008055:Lipids; D011137:Polystyrenes; D012333:RNA, Messenger; C494661:Serpinh1 protein, mouse; D015060:1,2-Dipalmitoylphosphatidylcholine; D003094:Collagen