title: Phenotypic and functional outcome of human monocytes or monocyte-derived dendritic cells in a dermal equivalent.
abstract: The dermis harbors a true dendritic cell population that could elicit primary allogeneic T cell responses in vitro and contact hypersensitivity reactions in vivo. The origin of dermal dendritic cells remains poorly understood, however. In this study, we analyzed the fate of monocytes or monocyte-derived dendritic cells in a dermal equivalent. Freshly isolated monocytes or monocytes cultured for 6 d with either GM-CSF/IL-4 or GM-CSF/IL-4/TGF-beta 1 (TGF-DC) were seeded in a collagen solution with normal human fibroblasts. The lattices were cultured for 7--14 d in the presence, or absence, of the exogenous cytokines, before phenotypic and functional studies were performed. Supply of exogenous cytokines allows the appearance of typical CD1a(+)/CD14(-)/CD68(low) dendritic cells with significant allostimulatory property, regardless of the cell type incorporated into the lattices. In cytokine-free conditions, monocytes and GM-CSF/IL-4-derived dendritic cells give rise to a CD1a(-)/CD14(+)/CD68(high) monocyte/macrophage population with no allostimulatory property. When incorporated into the lattices in the absence of exogenous cytokines the TGF-DC express few CD68 and FXIIIa. Interestingly, these cells do not all convert into the CD14(+)/CD1a(-) population. Indeed, a small HLA-DR(+)/CD1a(+)/CD14(-) subset was consistently found, which represents about one-third of the HLA-DR(+) cells. Moreover, TGF-DC recovered from the lattices after culture without cytokines do display a significant allostimulatory function. Thus, in the absence of exogenous cytokines, only Langerhans-cell-like dendritic cells can retain the typical dendritic cell features when inserted in a dermal environment. Taken together, these results may provide evidence supporting an epidermal origin of dermal dendritic cells.
authors.affiliation: Department of Dermatology, INSERM U346, Hôpital E. Herriot, Lyon, France.
authors.forename: G
authors.initials: G
authors.lastname: Guironnet
authors.forename: C
authors.initials: C
authors.lastname: Dezutter-Dambuyant
authors.forename: A
authors.initials: A
authors.lastname: Gaudillère
authors.forename: S
authors.initials: S
authors.lastname: Maréchal
authors.forename: D
authors.initials: D
authors.lastname: Schmitt
authors.forename: J
authors.initials: J
authors.lastname: Péguet-Navarro
mesh_terms: D018949:Antigens, CD1 / Q000032:analysis; D003713:Dendritic Cells / Q000502:physiology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D015847:Interleukin-4 / Q000494:pharmacology; D018950:Lipopolysaccharide Receptors / Q000032:analysis; D009000:Monocytes / Q000502:physiology*; D010641:Phenotype; D012867:Skin / Q000166:cytology* / Q000276:immunology
chemical_list: D018949:Antigens, CD1; C419393:CD1a antigen; D006684:HLA-DR Antigens; D018950:Lipopolysaccharide Receptors; D015847:Interleukin-4; D016178:Granulocyte-Macrophage Colony-Stimulating Factor