title: [Comparison of denaturing high performance liquid chromatography with direct sequencing in the detection of single nucleotide polymorphism].
abstract: OBJECTIVE
  Investigate the sensitivity and accuracy of the denaturing high performance liquid chromatography(DHPLC) technique for the detection of single nucleotide polymorphism(SNP).
  
  
  METHODS
  Forty-one samples were detected by both DHPLC and direct sequencing.
  
  
  RESULTS
  The comparison demonstrated that DHPLC detected all heterozygous sequences found by direct sequencing. No false-positive signals were seen in the cases of homozygous sequences. Furthermore, no false-negative results were ever obtained with heterozygous mutations or polymorphisms, or both.
  
  
  CONCLUSIONS
  DHPLC is a potent method for SNP identification especially SNP typing in large scale screening.
authors.affiliation: Chinese Human Genome Center at Shanghai, Shanghai 201203 P. R. China. huangwei@chgc.sh.cn
authors.forename: J
authors.initials: J
authors.lastname: Shi
authors.forename: S
authors.initials: S
authors.lastname: Yang
authors.forename: Z
authors.initials: Z
authors.lastname: Jiang
authors.forename: H
authors.initials: H
authors.lastname: Jiang
authors.forename: T
authors.initials: T
authors.lastname: Chen
authors.forename: Z
authors.initials: Z
authors.lastname: Chen
authors.forename: W
authors.initials: W
authors.lastname: Huang
mesh_terms: D002851:Chromatography, High Pressure Liquid; D006801:Humans; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA