C-27 and C-3 glucosylation of diosgenin by cell suspension cultures of Costus speciosus.
3-O-[beta-D-glucopyranosyl-(1"--> 2')-beta-D-glucopyranosyl], 27-O-beta-D-glucopyranosyl-(25R)-spirost-5-ene-3beta,27-diol was isolated from cell suspension cultures of Costus speciosus, following incubation with diosgenin, and its structure was elucidated using a combination of one- and two-dimensional 1H and 13C NMR spectral data, and positive and negative ion ESMS spectral data.
Indrayanto
G
G
Laboratory of Pharmaceutical Biotechnology, Faculty of Pharmacy, Airlangga University, Surabaya, Indonesia. indrayanto@hotmail.com
Zumaroh
S
S
Syahrani
A
A
Wilkins
A L
AL
D001711:Biotransformation; D002240:Carbohydrate Sequence; D002478:Cells, Cultured; D046508:Culture Techniques; D004144:Diosgenin / Q000302:isolation & purification / Q000378:metabolism*; D005960:Glucosides / Q000378:metabolism*; D006031:Glycosylation; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010636:Phenols / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry; D019862:Zingiberales / Q000737:chemistry*
D005960:Glucosides; D010636:Phenols; D010936:Plant Extracts; D004144:Diosgenin