title: Enantioselective determination of metoprolol and major metabolites in human urine by capillary electrophoresis.
abstract: The enantiomeric separation of metoprolol and its metabolites in human urine was undertaken using capillary electrophoresis (CE). Resolution of the enantiomers was achieved using carboxymethyl-beta-cyclodextrin (CM-beta-CD) as the chiral selector. A 100-mM acetate buffer (pH 4.0) containing 5% 2-propanol and 10 mM CM-beta-CD resulted in the optimum separation of the metoprolol enantiomers and its acidic metabolite in human urine. Following a single metoprolol oral administration of 100 mg racemic metoprolol tartrate, stereoselective pharmacokinetic analysis showed that urinary acidic metabolite 3 of metoprolol accounted for 62.3% of the dose with an R/S ratio of 1.23 and urinary unchanged metoprolol 1 accounted for 6.3% of the dose with an R/S ratio of 0.72.
authors.affiliation: College of Pharmacy, Chungnam National University, Taejon, South Korea.
authors.forename: H K
authors.initials: HK
authors.lastname: Lim
authors.forename: P T
authors.initials: PT
authors.lastname: Linh
authors.forename: C H
authors.initials: CH
authors.lastname: Hong
authors.forename: K H
authors.initials: KH
authors.lastname: Kim
authors.forename: J S
authors.initials: JS
authors.lastname: Kang
mesh_terms: D000319:Adrenergic beta-Antagonists / Q000652:urine*; D002021:Buffers; D019075:Electrophoresis, Capillary / Q000379:methods; D006801:Humans; D008790:Metoprolol / Q000378:metabolism / Q000493:pharmacokinetics / Q000652:urine*; D015394:Molecular Structure; D015203:Reproducibility of Results; D013237:Stereoisomerism
chemical_list: D000319:Adrenergic beta-Antagonists; D002021:Buffers; D008790:Metoprolol