title: Comparison of bacteriological, genetic and pathological characters between Escherichia coli O115a,c:K(B) and Citrobacter rodentium.
abstract: Murine pathogenic Escherichia coli O115a,c:K(B) (MPEC) is the causative agent of mouse megaenteron, the pathology of which resembles that of transmissible murine colonic hyperplasia caused by Citrobacter rodentium. We compared their genetic and pathological features to reveal the relationship between these two bacteria. To evaluate the genetic distances, 16S rDNA genes were sequenced and biochemical reactions were tested. Mouse strain susceptibility tests, using CF1 MPEC-susceptible germfree mice and BALB/cA(Jic) resistant mice were performed. MPEC strains and C. rodentium showed more than 99.6% identity by comparison of 16S rDNA gene sequences. All results from biochemical reactions and the mouse strain susceptibility tests were identical. It is proposed that MPEC should be reclassified as C. rodentium.
authors.affiliation: Laboratory of Veterinary, Public Health, Graduate School of Agriculture and Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657.
authors.forename: A
authors.initials: A
authors.lastname: Okutani
authors.forename: T
authors.initials: T
authors.lastname: Tobe
authors.forename: C
authors.initials: C
authors.lastname: Sasakawa
authors.forename: R
authors.initials: R
authors.lastname: Nozu
authors.forename: K
authors.initials: K
authors.lastname: Gotoh
authors.forename: A
authors.initials: A
authors.lastname: Takakura
authors.forename: T
authors.initials: T
authors.lastname: Itoh
authors.forename: Y
authors.initials: Y
authors.lastname: Miyamoto
authors.forename: K
authors.initials: K
authors.lastname: Itoh
mesh_terms: D000818:Animals; D016971:Citrobacter freundii / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity; D003106:Colon / Q000473:pathology; D003108:Colonic Diseases / Q000382:microbiology; D004269:DNA, Bacterial / Q000737:chemistry; D004198:Disease Susceptibility; D004926:Escherichia coli / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity; D005260:Female; D005856:Germ-Free Life; D006965:Hyperplasia / Q000382:microbiology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012376:Rodent Diseases / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S