Development of a human DNA quantitation system.
The AluQuant Human DNA Quantitation System has been developed for human-specific quantitation of forensic samples. This system uses probes specific to repetitive genetic elements allowing quantitation without target amplification. Target immobilization is unnecessary with employment of solution hybridization. The AluQuant Human DNA Quantitation System uses a series of enzymatic reactions to produce a luminescent signal proportional to the quantity of human DNA present. This report demonstrates a range of quantitation from 0.1-50 ng of human DNA. Signal from non-human DNAs tested was insignificant and addition of non-human DNAs into a human sample did not alter quantitation. Lastly, the system was unaffected by degradation of sample through sonication. The AluQuant Human DNA Quantitation System is a simple and sensitive method for quantitating the concentration of human DNA in forensic samples.
Mandrekar
M N
MN
Research and Development, Genetic Analysis, Promega Corporation, 2800 Woods Hollow Road, Madison, WI 53711-5399, USA. mmandrek@promega.com
Erickson
A M
AM
Kopp
K
K
Krenke
B E
BE
Mandrekar
P V
PV
Nelson
R
R
Peterson
K
K
Shultz
J
J
Tereba
A
A
Westphal
N
N
D004247:DNA / Q000032:analysis*; D016172:DNA Fingerprinting / Q000379:methods / Q000592:standards*; D005260:Female; D005554:Forensic Medicine / Q000379:methods*; D006801:Humans; D008297:Male; D021141:Nucleic Acid Amplification Techniques; D016133:Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity
D004247:DNA