title: Fluorescence immunoassay of alpha-fetoprotein with iron(III) tetrasulfonatophthalocyanine as a mimetic enzyme labeling reagent.
abstract: A new fluorimetric immunoassay for alpha-fetoprotein (AFP) has been developed using a novel promising mimetic peroxidase, iron(III) tetrasulfonatophthalocyanine (FeTSPc), as a labeling reagent to catalyze the fluorescence reaction of P- hydroxyphenylacetic acid (P-HPA) and hydrogen peroxide (H2O2). In the competitive immunoassay, anti-AFP antibody was coated on a 96-well plate (polystyrene) and a constant amount of FeTSPc-labeled AFP and a known amount of test solution were added. Non-labeled and FeTSPc-labeled AFP compete for binding to the plate-bound antibody. After the immunoreaction, the immunochemically adsorbed FeTSPc-AFP conjugate moiety was determined by measuring the fluorescence produced in a solution containing P-HPA and H2O2. AFP can be determined in the concentration range of 1-300 ng mL(-1) with a detection limit of 0.5 ng mL(-1).
authors.affiliation: Department of Chemistry, Xiamen University, P.R. China.
authors.forename: H H
authors.initials: HH
authors.lastname: Yang
authors.forename: Q Z
authors.initials: QZ
authors.lastname: Zhu
authors.forename: D H
authors.initials: DH
authors.lastname: Li
authors.forename: P
authors.initials: P
authors.lastname: Lin
authors.forename: M T
authors.initials: MT
authors.lastname: Ding
authors.forename: J G
authors.initials: JG
authors.lastname: Xu
mesh_terms: D000906:Antibodies; D000936:Antigen-Antibody Complex; D001667:Binding, Competitive; D004306:Dose-Response Relationship, Immunologic; D005290:Ferric Compounds / Q000138:chemical synthesis* / Q000737:chemistry; D015200:Fluoroimmunoassay / Q000379:methods*; D006735:Horseradish Peroxidase; D006801:Humans; D008665:Metalloporphyrins / Q000138:chemical synthesis* / Q000737:chemistry; D010648:Phenylacetates / Q000737:chemistry; D000509:alpha-Fetoproteins / Q000032:analysis* / Q000276:immunology
chemical_list: D000906:Antibodies; D000936:Antigen-Antibody Complex; D005290:Ferric Compounds; D008665:Metalloporphyrins; D010648:Phenylacetates; D000509:alpha-Fetoproteins; C430149:iron(III) tetrasulfonatophthalocyanine; C008070:4-hydroxyphenylacetic acid; D006735:Horseradish Peroxidase