title: Chromatographic purification of some 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitors.
abstract: The purification of pravastatin, simvastatin and lovastatin in the sodium salt or lactone form and of mevastatin in the lactone form by reversed-phase displacement chromatography is presented. The mobile phases consisted of water or mixtures of water-methanol and water-acetonitrile. Six different displacers were successfully used. Up to 0.14 g of raw sample per gram of stationary phase was loaded on a column packed with silica-based octadecyl phase. Crude substances from 85 to 88% chromatographic purity were purified and at least 99.5% purity was achieved.
authors.affiliation: Lek d.d., Research and Development, Ljubljana, Slovenia.
authors.forename: R
authors.initials: R
authors.lastname: Grahek
authors.forename: D
authors.initials: D
authors.lastname: Milivojevic
authors.forename: A
authors.initials: A
authors.lastname: Bastarda
authors.forename: M
authors.initials: M
authors.lastname: Kracun
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors / Q000302:isolation & purification*; D008148:Lovastatin / Q000031:analogs & derivatives* / Q000302:isolation & purification*; D017035:Pravastatin / Q000302:isolation & purification*; D019821:Simvastatin / Q000302:isolation & purification*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; C012258:mevastatin; D008148:Lovastatin; D019821:Simvastatin; D017035:Pravastatin