The polycystin-1 C-type lectin domain binds carbohydrate in a calcium-dependent manner, and interacts with extracellular matrix proteins in vitro.
Mutations in the PKD1 gene are responsible for 85% of cases of autosomal dominant polycystic kidney disease (ADPKD). This gene encodes a large membrane associated glycoprotein, polycystin-1, which is predicted to contain a number of extracellular protein motifs, including a C-type lectin domain between amino acids 403--532. We have cloned and expressed the PKD1 C-type lectin domain, and have demonstrated that it binds carbohydrate matrices in vitro, and that Ca(2+) is required for this interaction. This domain also binds to collagens type I, II and IV in vitro. This binding is greatly enhanced in the presence of Ca(2+) and can be inhibited by soluble carbohydrates such as 2-deoxyglucose and dextran. These results suggest that polycystin-1 may be involved in protein-carbohydrate interactions in vivo. The data presented indicate that there may a direct interaction between the PKD1 gene product and an ubiquitous extracellular matrix (ECM) protein.
Weston
B S
BS
Division of Life Sciences, King's College London, 150 Stamford Street, London SE1 9NN, UK.
Bagnéris
C
C
Price
R G
RG
Stirling
J L
JL
D000595:Amino Acid Sequence; D002118:Calcium / Q000737:chemistry / Q000378:metabolism*; D050260:Carbohydrate Metabolism; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000737:chemistry; D002413:Cations, Divalent; D002460:Cell Line; D003094:Collagen / Q000737:chemistry / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D037102:Lectins / Q000737:chemistry*; D037181:Lectins, C-Type; D008969:Molecular Sequence Data; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000737:chemistry; D016415:Sequence Alignment; D015398:Signal Transduction; D050396:TRPP Cation Channels
D002118:Calcium; D002241:Carbohydrates; D002413:Cations, Divalent; D003094:Collagen; D016326:Extracellular Matrix Proteins; D037102:Lectins; D037181:Lectins, C-Type; D011506:Proteins; D011993:Recombinant Fusion Proteins; D050396:TRPP Cation Channels