title: Antioxidant enzyme peroxiredoxin 5 is upregulated in degenerative human tendon.
abstract: Peroxiredoxin 5 (PRDX5) is a novel thioredoxin peroxidase recently identified in a variety of human cells and tissues, which is considered to play an important role in oxidative stress protection mechanisms. However, little is known about its expression in tendon degeneration, a common and disabling condition that primarily affects older people, in which oxidative stress may be implicated. The present study demonstrated that normal human tendon expresses PRDX5 and its expression is significantly increased in degenerative tendon. In addition, we have localized PRDX5 to fibroblasts in normal tendon and to both fibroblasts and endothelial cells in degenerate tendon. The differential expression of PRDX5 in normal and degenerate tendon shows that a thioredoxin peroxidase with antioxidant properties is upregulated under pathophysiological conditions and suggests that oxidative stress may be involved in the pathogenesis of tendon degeneration. PRDX5 may play a protective role against oxidative stress during this pathophysiological process.
authors.affiliation: Orthopaedic Research Institute, St. George Hospital, University of New South Wales, 4-10 South Street, Sydney, New South Wales 2217, Australia.
authors.forename: M X
authors.initials: MX
authors.lastname: Wang
authors.forename: A
authors.initials: A
authors.lastname: Wei
authors.forename: J
authors.initials: J
authors.lastname: Yuan
authors.forename: A
authors.initials: A
authors.lastname: Clippe
authors.forename: A
authors.initials: A
authors.lastname: Bernard
authors.forename: B
authors.initials: B
authors.lastname: Knoops
authors.forename: G A
authors.initials: GA
authors.lastname: Murrell
mesh_terms: D005347:Fibroblasts / Q000201:enzymology; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D018384:Oxidative Stress; D010544:Peroxidases / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D054464:Peroxiredoxins; D012333:RNA, Messenger / Q000096:biosynthesis; D013708:Tendon Injuries / Q000201:enzymology*; D013710:Tendons / Q000166:cytology / Q000201:enzymology; D015854:Up-Regulation
chemical_list: D012333:RNA, Messenger; D010544:Peroxidases; C515974:PRDX5 protein, human; D054464:Peroxiredoxins