The incorporation of wrong bases by DNA polymerase I following gamma-irradiation of DNA-like templates.
The synthesis of polydeoxyribose polymers by Escherichia coli DNA polymerase I has been investigated with control and gamma-irradiated DNA-like polymer templates containing only two bases. The results show that irradiation of a poly(dA) strand leads to the incorporation of dG, whereas irradiation of poly(dC) and poly(dG) strands both lead to the incorporation of dA. Irradiation of poly(dT) does not lead to the incorporation of any wrong base. The wrong bases are incorporated into the complementary strand of the newly synthesised DNA.
Saffhill
R
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Paterson Laboratories, Christie Hospital and Holt Radium Institute, Manchester M20 9BX, Great Britain.
D001665:Binding Sites; D004249:DNA Damage; D004256:DNA Polymerase I / Q000737:chemistry / Q000378:metabolism*; D018076:DNA, Complementary / Q000737:chemistry; D004307:Dose-Response Relationship, Radiation; D004926:Escherichia coli; D005720:Gamma Rays; D011061:Poly A / Q000737:chemistry; D011089:Polydeoxyribonucleotides / Q000737:chemistry / Q000528:radiation effects; D013698:Templates, Genetic
D018076:DNA, Complementary; D011089:Polydeoxyribonucleotides; C098036:poly(d(A-T))2; C093830:poly(dT)-2poly(dA); D011061:Poly A; C015465:poly(dA); C016681:poly(dG).poly(dC); D004256:DNA Polymerase I