Streptomycetes in micro-cultures: growth, production of secondary metabolites, and storage and retrieval in the 96-well format.
Mycelium-forming Streptomyces strains were grown in one milliliter liquid micro-cultures in square deep-well microtiter plates. Growth was evaluated with respect to biomass formation and production of secondary metabolites which were found to be very similar in the micro-cultures, bioreactor, and shake flask cultivations, respectively. Despite repetitive sampling and extensive growth on the walls of the wells, no cross contamination occurred. Furthermore, we successfully employed cold storage at -20 degrees C of spore suspensions (in the 96-well format), directly prepared from cultures grown on agar in the microtitre plate. Cultures were retrieved by replicating aliquots from the frozen spore suspensions.
Minas
W
W
Institute of Biotechnology, ETH-Zürich, Switzerland. minas@biotech.biol.ethz.ch
Bailey
J E
JE
Duetz
W
W
D001431:Bacteriological Techniques / Q000379:methods*; D018533:Biomass; D003470:Culture Media; D007700:Kinetics; D013171:Spores, Bacterial; D013302:Streptomyces / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D013997:Time Factors
D003470:Culture Media