Comparison of polymerase chain reaction on fresh tissue samples and fecal drops on filter paper for detection of Trypanosoma cruzi in Rhodnius prolixus.
PCR detection of Trypanosoma cruzi in Rhodnius prolixus using fresh tissue or fecal drops on filter paper showed comparable results: 38.7% infection rate using the fresh tissue sample and 37.9% by dried fecal drop.
Dorn
P L
PL
Department of Biological Sciences, Loyola University, New Orleans, LA, USA. dorn@loyno.edu
Flores
J
J
Brahney
B
B
Gutierrez
A
A
Rosales
R
R
Rodas
A
A
Monroy
C
C
D000818:Animals; D004587:Electrophoresis, Agar Gel; D005243:Feces / Q000469:parasitology*; D005374:Filtration; D007303:Insect Vectors / Q000469:parasitology; D007422:Intestines / Q000469:parasitology*; D010209:Paper; D016133:Polymerase Chain Reaction / Q000379:methods*; D012239:Rhodnius / Q000469:parasitology*; D014349:Trypanosoma cruzi / Q000302:isolation & purification*