Tracking of phenol degrading genotype.
A simple and rapid protocol has been developed for monitoring of the phenol degrading population in the environmental samples. PCR protocol uses the total DNA prepared from the samples as a template in the amplification reaction. Primers have been designed from the sequence data for Dmp and Phe operon encoding multi-component and single component phenol oxidizing system, respectively. The phenol degrading genotype in various samples have been demonstrated using the developed PCR protocol.
Kapley
A
A
Purohit
H J
HJ
D004269:DNA, Bacterial / Q000235:genetics; D004784:Environmental Monitoring / Q000379:methods; D004785:Environmental Pollutants / Q000378:metabolism*; D009876:Operon / Q000235:genetics; D010636:Phenols / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000379:methods; D011549:Pseudomonas / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D012680:Sensitivity and Specificity; D013698:Templates, Genetic
D004269:DNA, Bacterial; D004785:Environmental Pollutants; D010636:Phenols