Cloning, cellular distribution and functional expression of small intestinal epithelium guinea pig ClC-5 chloride channel.
We report the cloning of a guinea pig ClC-5 chloride channel (gpClC-5) from distal small intestinal epithelial cells by RT-PCR and RACE. The transcript is shown to be present in duodenum, jejunum and ileum epithelium by RT-PCR and Northern analysis. This is confirmed by in situ hybridisation which also shows the transcript to be homogeneously distributed in the crypt and villus regions. Expression of gpClC-5 in HEK-293 cells generated markedly outwardly rectified chloride currents with a perm-selectivity sequence of NO(3)(-)>Cl(-)>Br(-)>I(-)>F(-)>gluconate(-). The possible role of gpClC-5 in this epithelial location is discussed.
Cornejo
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I
Centro de Estudios Cientificos, Casilla, Valdivia, Chile.
Niemeyer
M I
MI
Sepúlveda
F V
FV
Cid
L P
LP
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology; D018118:Chloride Channels / Q000737:chemistry / Q000235:genetics* / Q000502:physiology*; D003001:Cloning, Molecular; D006168:Guinea Pigs; D006801:Humans; D017403:In Situ Hybridization; D007413:Intestinal Mucosa / Q000201:enzymology*; D007421:Intestine, Small / Q000201:enzymology; D007668:Kidney; D008536:Meglumine / Q000494:pharmacology; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic; D014162:Transfection
C097503:CLC-5 chloride channel; D018118:Chloride Channels; C115376:ClC-3 channel; D011994:Recombinant Proteins; D008536:Meglumine