Identification of essential residues for catalysis of rat intestinal phospholipase B/lipase.
Intestinal brush border membrane-associated phospholipase B/lipase (PLB/LIP) consists of four tandem homologous domains (repeats 1 through 4) and a COOH-terminal membrane binding domain, and repeat 2 is the catalytic domain that catalyzes phospholipase A2, lysophospholipase, and lipase activities. We examined the structural basis of the catalysis of PLB/LIP with this unique substrate specificity by site-directed mutagenesis of recombinant repeat 2 enzyme. Ser414 and Ser459 within the active serine-containing consensus sequence G-X-S-X-G in the best-established lipase family were dispensable for activity. In contrast, substitution of Ala for Ser404 almost completely inactivated the three lipolytic activities of PLB/LIP, even though the gross conformation was not altered as determined by CD spectroscopy. Notably, this Ser is located within the conserved G-D-S-L sequence on the NH2-terminal side in lipolytic enzymes of another group proposed recently. Furthermore, mutagenesis and CD spectroscopic analyses suggested that Asp518 and His659, lying within conserved short stretches in the latter group of lipolytic enzymes, were essential for activity. These three essential residues are conserved in the known PLB/LIP enzymes, suggesting that they form the catalytic triad in the active site. These results indicate that PLB/LIP represents a distinct class of the lipase family. PLB/LIP is the first mammalian member of that family. Repeat 2 is equipped with the triad, but not the other repeats, accounting for why only repeat 2 is the catalytic domain. Replacing Thr406 with Gly, matching the enzyme's sequence to the lipase consensus sequence exactly, led to a great decrease in secretion and accumulation of inactive enzyme in the cells, suggesting a role of Thr406 in the structural stability.
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Department of Molecular Physiological Chemistry, Osaka University Medical School, 2-2 Yamadaoka, Suita, Osaka 565-0785, Japan.
Ito
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Tchoua
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Takemori
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Okamoto
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Tojo
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D000596:Amino Acids / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001224:Aspartic Acid / Q000235:genetics / Q000378:metabolism; D019556:COS Cells; D020134:Catalytic Domain / Q000235:genetics; D004789:Enzyme Activation / Q000235:genetics; D005998:Glycine / Q000235:genetics; D006168:Guinea Pigs; D006639:Histidine / Q000235:genetics / Q000378:metabolism; D007422:Intestines / Q000201:enzymology*; D008245:Lysophospholipase / Q000235:genetics / Q000378:metabolism*; D016297:Mutagenesis, Site-Directed; D011817:Rabbits; D051381:Rats; D020449:Repetitive Sequences, Amino Acid; D012694:Serine / Q000235:genetics / Q000378:metabolism; D013912:Threonine / Q000235:genetics
D000596:Amino Acids; D013912:Threonine; D001224:Aspartic Acid; D012694:Serine; D006639:Histidine; C110540:phospholipase B-lipase; D008245:Lysophospholipase; D005998:Glycine