High-performance liquid chromatographic assay of zonisamide in human plasma using a non-porous silica column.
A new method for measuring zonisamide (ZNS) in plasma by high-performance liquid chromatography was developed by using a 2-microm reversed-phase non-porous silica column. ZNS in plasma was first purified with a column extraction technique and injected onto the non-porous silica column. Calibration curve was linear over the concentration range of 1-80 microg/ml in plasma. The recoveries of ZNS added to plasma were more than 95.4% with the coefficient of variation less than 9.0%. We developed a rapid routine method using the non-porous silica column that was accurate and improved solvent consumption in the measurement of ZNS.
Nakamura
M
M
Department of Pharmacy, Gifu University Hospital, Japan.
Hirade
K
K
Sugiyama
T
T
Katagiri
Y
Y
D000927:Anticonvulsants / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D007555:Isoxazoles / Q000097:blood* / Q000493:pharmacokinetics; D012822:Silicon Dioxide; D012997:Solvents; D000078305:Zonisamide
D000927:Anticonvulsants; D007555:Isoxazoles; D012997:Solvents; D000078305:Zonisamide; D012822:Silicon Dioxide