title: Level of Flt3-ligand in plasma: a possible new bio-indicator for radiation-induced aplasia.
abstract: OBJECTIVE
  To follow plasma Flt3-ligand (FL) concentrations in irradiated animals in order to evaluate it as an indicator of bone marrow damage for the management of accidental radiation-induced aplasia.
  
  
  METHODS
  Non-human primates were irradiated at doses ranging from 2 to 8 Gy, using whole- or partial-body irradiation. Plasma FL concentrations and blood cell counts were determined daily.
  
  
  RESULTS
  FL concentrations increased as early as day 2 after irradiation, whatever the irradiation dose. Increase in plasma FL concentration on day 5 post-irradiation was correlated with radiation dose and with the severity of radiation-induced aplasia. During the course of aplasia, FL concentrations in plasma were inversely correlated with neutrophil counts. A peak in FL concentration appeared before the neutrophil nadir, and the subsequent decrease in FL concentration was correlated with the recovery of blood-cell populations.
  
  
  CONCLUSIONS
  Monitoring plasma FL concentration can be used as an indicator of radiation-induced marrow aplasia, and this may be of use in accidental irradiation situations.
authors.affiliation: Institut de Protection et de Sûreté Nucléaire, Département de Protection de la Santé de L'homme et de Dosimétrie, Fontenay-aux-Roses Cedex, France.
authors.forename: J M
authors.initials: JM
authors.lastname: Bertho
authors.forename: C
authors.initials: C
authors.lastname: Demarquay
authors.forename: J
authors.initials: J
authors.lastname: Frick
authors.forename: C
authors.initials: C
authors.lastname: Joubert
authors.forename: S
authors.initials: S
authors.lastname: Arenales
authors.forename: N
authors.initials: N
authors.lastname: Jacquet
authors.forename: I
authors.initials: I
authors.lastname: Sorokine-Durm
authors.forename: Q
authors.initials: Q
authors.lastname: Chau
authors.forename: M
authors.initials: M
authors.lastname: Lopez
authors.forename: J
authors.initials: J
authors.lastname: Aigueperse
authors.forename: N C
authors.initials: NC
authors.lastname: Gorin
authors.forename: P
authors.initials: P
authors.lastname: Gourmelon
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood; D001853:Bone Marrow / Q000293:injuries / Q000528:radiation effects; D001855:Bone Marrow Diseases / Q000097:blood* / Q000209:etiology / Q000628:therapy; D016026:Bone Marrow Transplantation; D002940:Circadian Rhythm; D003114:Colony-Forming Units Assay; D003429:Cross Reactions; D005260:Female; D006801:Humans; D007958:Leukocyte Count; D008252:Macaca fascicularis; D008297:Male; D008565:Membrane Proteins / Q000097:blood* / Q000276:immunology; D009504:Neutrophils; D010976:Platelet Count; D011518:Proto-Oncogene Proteins / Q000276:immunology; D011833:Radiation Injuries, Experimental / Q000097:blood* / Q000209:etiology / Q000628:therapy; D020794:Receptor Protein-Tyrosine Kinases / Q000276:immunology; D014182:Transplantation, Autologous; D014916:Whole-Body Irradiation; D051941:fms-Like Tyrosine Kinase 3
chemical_list: D015415:Biomarkers; D008565:Membrane Proteins; D011518:Proto-Oncogene Proteins; C084881:flt3 ligand protein; C497970:FLT3 protein, human; D020794:Receptor Protein-Tyrosine Kinases; D051941:fms-Like Tyrosine Kinase 3