Bioskin as an affinity matrix for the separation of glycoproteins.
Bioskin is a natural product produced by a mixed culture of Acetobacter xylinum, Saccharomyces cerevisiae and S. pombe cultured on media containing sucrose. It is of fibrillar nature able to retain some proteins, such as cytochrome c, by adsorption, and mainly composed of glucosamine and N-acetyl-D-glucosamine. This makes it possible that, at an adequate pH value, proteins charged as polyanionic molecules, such as catalase, can be retained by ionic adsorption using the positively charged amino groups of the matrix. In addition, bioskin can also be used as an affinity matrix to retain glycoproteins able to perform specific affinity reactions with the amino sugars of the matrix, such as invertase, fetuin or ovalbumin. Its possible use as a chromatographic support is discussed.
Vicente
C
C
Department of Plant Physiology, Faculty of Biology, Complutense University, Madrid, Spain.
Sebastián
B
B
Fontaniella
B
B
Márquez
A
A
Xavier Filho
L
L
Legaz
M E
ME
D000327:Adsorption; D002374:Catalase / Q000737:chemistry / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D019075:Electrophoresis, Capillary; D005944:Glucosamine / Q000737:chemistry*; D006023:Glycoproteins / Q000302:isolation & purification*; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet
D006023:Glycoproteins; C437163:bioskin; D002374:Catalase; D005944:Glucosamine