Characterization of vitronectin-binding proteins of Staphylococcus epidermidis.
Staphylococcus epidermidis is the most common microorganism that is isolated from the cerebrospinal fluid (CSF) shunt infection patients. Vitronectin adsorbed on the surface of implants may mediate bacterial adhesion and colonization. To characterize vitronectin-binding properties, we analyzed S. epidermidis BD5703 isolated from a CSF shunt infection. Expression of vitronectin-binding protein(s) depended on culture media. Two proteins (60 and 52 kDa) were purified from vitronectin affinity chromatography. Two other vitronectin-binding proteins (21 and 16 kDa) were purified from an ion-exchange column. All purified proteins blocked bacterial binding of immobilized vitronectin significantly except the 16-kDa protein. The N-terminal sequences of the 21- and 16-kDa proteins did not show any appreciable amino acid sequence homology. The 52-kDa protein was sequenced by mass spectrometry and identified as an autolysin. This report demonstrates that interaction of vitronectin with multiple recognition sites on BD5703 surface may contribute to bacterial colonization.
Li
D Q
DQ
Department of Infectious Diseases and Medical Microbiology, Lund University Hospital, Sölvegatan 23, S-223 62 Lund, Sweden.
Lundberg
F
F
Ljungh A
D001345:Autoradiography / Q000379:methods; D001426:Bacterial Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D002557:Cerebrospinal Fluid Shunts / Q000009:adverse effects; D004268:DNA-Binding Proteins; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D013203:Staphylococcal Infections / Q000382:microbiology; D013212:Staphylococcus epidermidis / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D019096:Vitronectin / Q000037:antagonists & inhibitors / Q000378:metabolism*
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D019096:Vitronectin; C094734:vitronectin-binding protein, Staphylococcus aureus