[Cloning of rat beta-defensin rBD-1 cDNA].
This is a study aimed at the molecular mechanisms of beta-defensins gene expression and it's gene transfer experiments for preventing mucosal infection. A rat rBD-1 cDNA was cloned. The total RNA was isolated from rat kidney. The cDNA fragment was amplified by RT-PCR with specific primers. The purified RT-PCR product was cloned in pGEM-T Easy vector. The results of restriction endonuclease pattern analysis of the recombinant plasmid, DNA sequencing, and aligning of the putative amino acid sequence with hBD-1 and mBD-1 demonstrated that rat beta-defensin rBD-1 gene was cloned successfully.
Huang
N
N
Research Unit of Infection & Immunity, School of Basic Medical Sciences, WCUMS, Chengdu 610041.
Tang
B
B
Wu
Q
Q
Chen
X
X
Pan
X
X
Wang
B
B
D000595:Amino Acid Sequence; D000818:Animals; D000890:Anti-Infective Agents; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D005786:Gene Expression Regulation; D007668:Kidney / Q000378:metabolism; D008969:Molecular Sequence Data; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D023083:beta-Defensins / Q000096:biosynthesis / Q000235:genetics*
D000890:Anti-Infective Agents; D018076:DNA, Complementary; D011994:Recombinant Proteins; D023083:beta-Defensins