Effects of dermatan and dextran sulfates on arginine amidase activity released from isolated rabbit arteries.
We examined the levels of arginine amidase secreted from isolated rabbit arteries treated with dermatan and dextran sulfates, and the relation between the secretion of arginine amidase activity and the concentration of dermatan sulfate added to these arteries. The results showed that while dextran sulfate tended to accelerate the release of arginine amidase activity from the isolated rabbit ear artery, the induction was not significant. There was a significant increase in the level of arginine amidase released from the lower portion of isolated rabbit aorta (p<0.05), but no significant change in the upper portion of the aorta. In contrast, the addition of dermatan sulfate significantly increased the level of arginine amidase activity released from the isolated rabbit ear artery and the upper and lower portions of the aorta (p<0.05). Linear dose-response relationships were observed between the level of arginine amidase activity released from the isolated rabbit ear artery and aorta and the concentration of dermatan sulfate added.
Takeuchi
K
K
Department of Clinical Pharmacology, and Functional Bioanalysis, Meiji Pharmaceutical University, Kiyose, Tokyo, Japan. koichita@my-pharm.ac.jp
Miyazaki
K
K
Ishii-Nozawa
R
R
Chiba
R
R
Shimokawa
K
K
Katayama
M
M
Matsuda
Y
Y
D000818:Animals; D001158:Arteries / Q000201:enzymology*; D003871:Dermatan Sulfate / Q000494:pharmacology*; D016264:Dextran Sulfate / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D066298:In Vitro Techniques; D011817:Rabbits; D012697:Serine Endopeptidases / Q000378:metabolism*
D003871:Dermatan Sulfate; D016264:Dextran Sulfate; D012697:Serine Endopeptidases; C402942:arginine amidase