title: Genome sequence of a VP2/NS junction region of pillar cell necrosis virus (PCNV) in cultured Japanese eel Anguilla japonica.
abstract: Pillar cell necrosis virus (PCNV) is an aquatic birnavirus that was isolated from farmed Japanese eel experiencing mass mortality. In this study, a VP2/NS junction region in genome segment A of PCNV was amplified by reverse transcriptase polymerase chain reaction (RT-PCR) and sequenced. The VP2/NS region in PCNV had the highest homology with that of a strain Ab of infectious pancreatic necrosis virus (IPNV). This result revealed that PCNV belongs to birnavirus genogroup II.
authors.affiliation: Department of Aquatic Life Medicine, Pukyong National University, Nam-gu, Pusan, Korea. namsill@yahoo.co.kr
authors.forename: N S
authors.initials: NS
authors.lastname: Lee
authors.forename: M
authors.initials: M
authors.lastname: Miyata
authors.forename: T
authors.initials: T
authors.lastname: Miyazaki
mesh_terms: D000811:Anguilla / Q000821:virology*; D000818:Animals; D001483:Base Sequence; D018059:Birnaviridae / Q000235:genetics*; D003001:Cloning, Molecular; D005393:Fish Diseases / Q000821:virology*; D016679:Genome, Viral; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D017361:Viral Nonstructural Proteins / Q000235:genetics*
chemical_list: D017361:Viral Nonstructural Proteins