title: Secretion of human growth hormone by the food-grade bacterium Staphylococcus carnosus requires a propeptide irrespective of the signal peptide used.
abstract: Staphylococcal exoproteins can be divided into two groups. One group comprises proteins bearing only a signal peptide, the other group requires an additional propeptide for secretion. The secretion signals of the propeptide-requiring lipase from Staphylococcus hyicus (Lip) have been frequently used to produce recombinant secretory proteins in the food-grade species Staphylococcus carnosus. However, it has been unclear whether recombinant proteins can be secreted using signal peptides of staphylococcal proteins without propeptide. The human growth hormone protein (hGH) was fused to various staphylococcal secretion signals of proteins without propeptide (Seb, SceA, and SceB) and of proteins requiring a propeptide (lipase, lysostaphin, and glycerol ester hydrolase). Secretory hGH was efficiently produced by S. carnosus after fusion with any propeptide-containing secretion signal, whereas precursor proteins were retained in the cells when only a signal peptide was used. Addition of the first six amino acid residues of mature SceA to the signal peptide did also not lead to secretion of hGH. It was concluded that the properties of the mature protein domains determine whether a propeptide is required for secretion or not. The Lip propeptide could be efficiently removed from hGH after introduction of an enterokinase cleavage site between the two protein domains.
authors.affiliation: Mikrobielle Genetik, Universität Tübingen, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Sturmfels
authors.forename: F
authors.initials: F
authors.lastname: Götz
authors.forename: A
authors.initials: A
authors.lastname: Peschel
mesh_terms: D000595:Amino Acid Sequence; D004765:Enteropeptidase / Q000378:metabolism; D019382:Human Growth Hormone / Q000378:metabolism*; D008969:Molecular Sequence Data; D021382:Protein Sorting Signals; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D013210:Staphylococcus / Q000378:metabolism*
chemical_list: D021382:Protein Sorting Signals; D011993:Recombinant Fusion Proteins; D019382:Human Growth Hormone; D004765:Enteropeptidase