BIACORE data processing: an evaluation of the global fitting procedure.
Data from real-time molecular interaction analysis using BIACORE are currently evaluated by numerical integration. We have investigated the ability of two software packages (BIAevaluation 3.0 and CLAMP99) to analyze complex interactions. Three experimental data sets of high quality obtained with BIACORE upgraded and 2000 instruments, representative of simple bimolecular, heterogeneous ligand, and mass-transport-limited interactions, were processed by the global fitting procedure. The two software, which differ mainly in the statistical assessment of the output values, were able to discriminate correctly between various interacting models and provided very close output parameters with satisfactory statistical tests.
Khalifa
M B
MB
Laboratoire d'Ingénierie des Macromolécules, Laboratoire de Biophysique Moléculaire, Institut de Biologie Structurale Jean-Pierre Ebel, 41 rue Jules Horowitz, Grenoble Cedex 1, 38027, France.
Choulier
L
L
Lortat-Jacob
H
H
Altschuh
D
D
Vernet
T
T
D000595:Amino Acid Sequence; D000937:Antigen-Antibody Reactions; D000941:Antigens / Q000378:metabolism; D001692:Biological Transport; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods*; D006801:Humans; D007700:Kinetics; D008024:Ligands; D008969:Molecular Sequence Data; D009010:Monte Carlo Method; D011485:Protein Binding; D012680:Sensitivity and Specificity; D012984:Software
D000941:Antigens; D008024:Ligands