title: The Aspergillus nidulans metE gene is regulated by a second system independent from sulphur metabolite repression.
abstract: Mutations in the Aspergillus nidulans metE gene lead to requirement for O-acetylhomoserine. The gene was cloned by complementation of the metE31 mutation. The coding sequence was found to be interrupted by two introns of 66 and 50 bp, respectively. metE codes for a peptide of 489 amino acids which belongs to the family of homoserine O-acetyltransferases and a well-defined superfamily of alpha/beta hydrolases. Transcription of the metE gene is strongly up-regulated by a severe limitation of methionine, but not of cysteine. This gene is the first sulphur metabolism gene described in A. nidulans which is not regulated by the sulphur metabolite repression system in which cysteine acts as the low-molecular-weight effector.
authors.affiliation: Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Grynberg
authors.forename: M
authors.initials: M
authors.lastname: Piotrowska
authors.forename: E
authors.initials: E
authors.lastname: Pizzinini
authors.forename: G
authors.initials: G
authors.lastname: Turner
authors.forename: A
authors.initials: A
authors.lastname: Paszewski
mesh_terms: D000595:Amino Acid Sequence; D001233:Aspergillus nidulans / Q000235:genetics* / Q000254:growth & development; D002244:Carbon; D003001:Cloning, Molecular; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D008715:Methionine / Q000172:deficiency / Q000378:metabolism; D008780:Methyltransferases / Q000235:genetics*; D008956:Models, Chemical; D008969:Molecular Sequence Data; D009584:Nitrogen; D017421:Sequence Analysis; D013455:Sulfur
chemical_list: D013455:Sulfur; D002244:Carbon; D008715:Methionine; D008780:Methyltransferases; C121199:methylcobalamin - homocysteine methyltransferase; D009584:Nitrogen