title: Mechanism of actin-based motility.
abstract: Spatially controlled polymerization of actin is at the origin of cell motility and is responsible for the formation of cellular protrusions like lamellipodia. The pathogens Listeria monocytogenes and Shigella flexneri, which undergo actin-based propulsion, are acknowledged models of the leading edge of lamellipodia. Actin-based motility of the bacteria or of functionalized microspheres can be reconstituted in vitro from only five pure proteins. Movement results from the regulated site-directed treadmilling of actin filaments, consistent with observations of actin dynamics in living motile cells and with the biochemical properties of the components of the synthetic motility medium.
authors.affiliation: Dynamique du Cytosquelette, Laboratoire d'Enzymologie et Biochimie Structurales, CNRS, Avenue de la Terrasse, 91198 Gif-sur-Yvette, France.
authors.forename: D
authors.initials: D
authors.lastname: Pantaloni
authors.forename: C
authors.initials: C
authors.lastname: Le Clainche
authors.forename: M F
authors.initials: MF
authors.lastname: Carlier
mesh_terms: D051339:Actin Depolymerizing Factors; D051377:Actin-Related Protein 2; D051378:Actin-Related Protein 3; D000199:Actins / Q000378:metabolism / Q000502:physiology*; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism; D001704:Biopolymers; D002465:Cell Movement; D003598:Cytoskeletal Proteins; D051345:Destrin; D008089:Listeria monocytogenes / Q000502:physiology*; D008840:Microfilament Proteins / Q000378:metabolism; D008954:Models, Biological; D009068:Movement; D011506:Proteins / Q000378:metabolism; D011554:Pseudopodia / Q000502:physiology; D015398:Signal Transduction; D051316:Wiskott-Aldrich Syndrome Protein
chemical_list: D051339:Actin Depolymerizing Factors; D051377:Actin-Related Protein 2; D051378:Actin-Related Protein 3; D000199:Actins; D001426:Bacterial Proteins; D001704:Biopolymers; D003598:Cytoskeletal Proteins; D051345:Destrin; D008840:Microfilament Proteins; D011506:Proteins; D051316:Wiskott-Aldrich Syndrome Protein; D000255:Adenosine Triphosphate