title: Preparation of recombinant coat protein of Prunus necrotic ringspot virus.
abstract: The coat protein (CP) gene of Prunus necrotic ringspot virus (PNRSV) was cloned into pET 16b vector and expressed in Escherichia coli. CP-enriched fractions were prepared from whole cell lysate by differential centrifugation. The fraction sedimenting at 20,000 x g for 30 mins was used for preparation of a rabbit antiserum to CP. This antiserum had a titer of 1:2048 and reacted in a double-antibody sandwich ELISA (DAS-ELISA).
authors.affiliation: Department of Plant Virology, Institute of Plant Molecular Biology, Academy of Sciences of the Czech Republic, Ceské Budejovice.
authors.forename: K
authors.initials: K
authors.lastname: Petrzik
authors.forename: I
authors.initials: I
authors.lastname: Mráz
authors.forename: D
authors.initials: D
authors.lastname: Kubelková
mesh_terms: D001483:Base Sequence; D002213:Capsid / Q000235:genetics / Q000302:isolation & purification*; D003001:Cloning, Molecular; D005814:Genes, Viral; D019179:Ilarvirus / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification; D014197:Trees / Q000821:virology
chemical_list: D011994:Recombinant Proteins