title: Modulation of carbon tetrachloride-induced lipid peroxidation and xenobiotic-metabolizing enzymes in rats fed browned yam flour diet.
abstract: The modulatory effect of browned yam flour diet, a dietary, staple in south-western Nigeria, on carbon tetrachloride (CCl4)-mediated lipid peroxidation and on the activities of liver microsomal and cytosolic enzymes was studied in male rats. Browned yam flour diet fed at the level of 25% and 50% to rats for 5 weeks significantly reduced the lipid peroxidation induced by CCl4 (0.5 ml/kg/wk) administered two weeks after starting the animals with the diets. The diets elicited 62% and 79% reductions in CCl4-mediated peroxidation, respectively, in the absence of exogenously added oxidants. The activities of microsomal aniline hydroxylase (AH), aminopyrine-N-demethylase (APD), pentoxyresorufin-O-dealkylase (PROD) and cytosolic GSH S-transferase (GST) were increased when rats were fed the 25% or 50% browned yam flour diets. Browned yam flour fed at the level of 25% to rats decreased the CCl4-mediated reduction in the activities of microsomal AH, APD, PROD and GST by 64%, 28%, 58% and 25%, respectively, and by 82%, 48%, 83% and 55% when rats were fed with 50% of the diet. The results suggest that browned yam flour diet could protect against chemically-mediated lipid peroxidation and tissue damage possibly by scavenging chemically generated reactive species and enhancing carcinogen-detoxifying system.
authors.affiliation: Department of Biochemistry, Drug Metabolism and Toxicology Unit, College of Medicine, University of Ibadan, Nigeria.
authors.forename: E O
authors.initials: EO
authors.lastname: Farombi
authors.forename: J O
authors.initials: JO
authors.lastname: Nwankwo
authors.forename: G O
authors.initials: GO
authors.lastname: Emerole
mesh_terms: D000633:Aminopyrine N-Demethylase / Q000032:analysis; D000815:Aniline Hydroxylase / Q000032:analysis; D000818:Animals; D002252:Carbon Tetrachloride Poisoning / Q000178:diet therapy* / Q000378:metabolism*; D019362:Cytochrome P-450 CYP2B1 / Q000032:analysis; D003600:Cytosol / Q000737:chemistry / Q000201:enzymology*; D004195:Disease Models, Animal; D004353:Drug Evaluation, Preclinical; D005433:Flour; D016166:Free Radical Scavengers / Q000627:therapeutic use*; D005982:Glutathione Transferase / Q000032:analysis; D019668:Liliaceae / Q000737:chemistry*; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D008862:Microsomes, Liver / Q000737:chemistry / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D015262:Xenobiotics / Q000378:metabolism* / Q000506:poisoning*
chemical_list: D016166:Free Radical Scavengers; D017392:Thiobarbituric Acid Reactive Substances; D015262:Xenobiotics; D000815:Aniline Hydroxylase; D019362:Cytochrome P-450 CYP2B1; D000633:Aminopyrine N-Demethylase; D005982:Glutathione Transferase