title: Focal atrial fibrillation: experimental evidence for a pathophysiologic role of the autonomic nervous system.
abstract: BACKGROUND
  Focal paroxysmal atrial fibrillation (AF) was shown recently to originate in the pulmonary veins (PVs) and superior vena cava (SVC). In the present study, we describe an animal model in which local high-frequency electrical stimulation produces focal atrial activation and AF/AT (atrial tachycardia) with electrogram characteristics consistent with clinical reports.
  
  
  RESULTS
  In 21 mongrel dogs, local high-frequency electrical stimulation was performed by delivering trains of electrical stimuli (200 Hz, impulse duration 0.1 msec) to the PVs/SVC during atrial refractoriness. Atrial premature depolarizations (APDs), AT, and AF occurred with increasing high-frequency electrical stimulation voltage. APD/AT/AF originated adjacent to the site of high-frequency electrical stimulation and were inducible in 12 of 12 dogs in the SVC and in 8 of 9 dogs in the left superior PV (left inferior PV: 7/8, right superior PV: 6/8; right inferior PV: 4/8). In the PVs, APDs occurred at 13+/-8 V and AT/AF at 15+/-9 V (P < 0.01; n = 25). In the SVC, APDs were elicited at 19+/-6 V and AT/AF at 26+/-6 V (P < 0.01; n = 12). High-frequency electrical stimulation led to local refractory period shortening in the PVs. The response to high-frequency electrical stimulation was blunted or prevented after beta-receptor blockade and abolished by atropine. In vitro, high-frequency electrical stimulation induced a heterogeneous response, with shortening of the action potential in some cells (from 89+/-35 msec to 60+/-22 msec; P < 0.001; n = 7) but lengthening of the action potential and development of early afterdepolar-izations that triggered APD/AT in other cells. Action potential shortening was abolished by atropine.
  
  
  CONCLUSIONS
  High-frequency electrical stimulation evokes rapid ectopic beats from the PV/SVC, which show variable degrees of conduction block to the atria and induce AF, resembling findings in patients with focal idiopathic paroxysmal AF. The occurrence of the arrhythmia in this animal model was likely due to alterations in local autonomic tone by high-frequency electrical stimulation. Further research is needed to prove absolutely that the observed effects of high-frequency electrical stimulation were caused by autonomic nerve stimulation.
authors.affiliation: Department of Internal Medicine, University of Oklahoma Health Sciences Center, Oklahoma City, USA. psch@pcserver.mk1.rwth-aachen.de
authors.forename: P
authors.initials: P
authors.lastname: Schauerte
authors.forename: B J
authors.initials: BJ
authors.lastname: Scherlag
authors.forename: E
authors.initials: E
authors.lastname: Patterson
authors.forename: M A
authors.initials: MA
authors.lastname: Scherlag
authors.forename: K
authors.initials: K
authors.lastname: Matsudaria
authors.forename: H
authors.initials: H
authors.lastname: Nakagawa
authors.forename: R
authors.initials: R
authors.lastname: Lazzara
authors.forename: W M
authors.initials: WM
authors.lastname: Jackman
mesh_terms: D000818:Animals; D001281:Atrial Fibrillation / Q000503:physiopathology*; D018880:Atrial Premature Complexes / Q000503:physiopathology; D001341:Autonomic Nervous System / Q000503:physiopathology; D004195:Disease Models, Animal; D004285:Dogs; D004558:Electric Stimulation / Q000295:instrumentation / Q000379:methods; D006325:Heart Atria / Q000503:physiopathology; D023421:Models, Animal; D011667:Pulmonary Veins / Q000503:physiopathology; D014683:Vena Cava, Superior / Q000503:physiopathology