Chemorepellent signaling through the PACAP/lysozyme receptor is mediated through cAMP and PKC in Tetrahymena thermophila.
Pituitary adenylate cyclase-activating peptide and lysozyme are potent chemorepellents which act through the same receptor in Tetrahymena. Using in vivo behavioral studies, we have found that the pituitary adenylate cyclase-activating peptide/lysozyme receptor appears to signal through a G-protein pathway which is mediated through both adenosine 3'5'monophosphate and protein kinase C. Avoidance to pituitary adenylate cyclase-activating peptide and lysozyme is inhibited by the G-protein inhibitor, guanosine 5'-O-(2thiodiphosphate), the adenosine 3'5'monophate analog, Rp-adenosine-3', 5' cyclic monophosphorothioate, and the protein kinase C inhibitors, calphostin C and bisindolylmaleimide IV. A proposed model for signaling through the pituitary adenylate cyclase-activating peptide/lysozyme receptor is briefly outlined.
Hassenzahl
D L
DL
Cedarville University, Department of Science and Mathematics, OH 45314, USA.
Yorgey
N K
NK
Keedy
M D
MD
Price
A R
AR
Hall
J A
JA
Myzcka
C C
CC
Kuruvilla
H G
HG
D000818:Animals; D001522:Behavior, Animal; D002628:Chemoreceptor Cells; D000242:Cyclic AMP / Q000494:pharmacology*; D008124:Locomotion; D009113:Muramidase / Q000494:pharmacology; D009479:Neuropeptides / Q000494:pharmacology*; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D011493:Protein Kinase C / Q000494:pharmacology*; D016808:Tetrahymena thermophila / Q000502:physiology*
D009479:Neuropeptides; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D000242:Cyclic AMP; D011493:Protein Kinase C; D009113:Muramidase