title: High-performance liquid chromatography coupled with chemiluminescence nitrogen detection for the study of ethoxyquin antioxidant and related organic bases.
abstract: The Chemiluminescent Nitrogen Detector (CLND) for use with high-performance liquid chromatography (HPLC) allows for the low-level detection of nitrogen-containing compounds with simple quantitation. The nitrogen selective detector's equimolar response (i.e., equal response for nitrogen independent of its chemical environment) allows for any nitrogen-containing compound to be quantitated as long as the number of nitrogens are known. The HPLC-CLND provides a new detection method for analytes that are not available in large quantities or have unknown chemical or physical characteristics such as oxidation products, metabolites, or impurities. Ethoxyquin is a primary antioxidant that is used to preserve many food products and animal feeds. HPLC-CLND is used in the study of the oxidation products of ethoxyquin because limited quantities of these compounds are available and subsequent calibration curves are difficult to maintain. HPLC-CLND as a new method of detection has been evaluated for its equimolarity of response, linear range, limit of detection, and limit of quantitation.
authors.affiliation: Department of Chemistry, Virginia Tech, Blacksburg 24061-0212, USA.
authors.forename: D
authors.initials: D
authors.lastname: Brannegan
authors.forename: M
authors.initials: M
authors.lastname: Ashraf-Khorassani
authors.forename: L T
authors.initials: LT
authors.lastname: Taylor
mesh_terms: D000975:Antioxidants / Q000737:chemistry*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005015:Ethoxyquin / Q000737:chemistry*; D008163:Luminescent Measurements; D009584:Nitrogen; D009930:Organic Chemicals / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000975:Antioxidants; D009930:Organic Chemicals; D005015:Ethoxyquin; D009584:Nitrogen