Changes in molecular weight of hyaluronan and hyaluronidase activity in uterine cervical mucus in cervical ripening.
BACKGROUND
This study was undertaken to investigate changes in the forms of hyaluronan and hyaluronidase activity in cervical mucus during cervical ripening.


METHODS
Uterine cervical mucus was obtained from 57 pregnant women (25 at preterm gestation, ten at term gestation, 11 within 1 week before labor, and 11 during the first stage of labor). We determined 1) concentration of hyaluronan, 2) hyaluronidase activity, and 3) molecular weight of hyaluronan in cervical mucus. Data are presented as mean and range.


RESULTS
The hyaluronan concentration in mucus in the 1st stage of labor (1.58 microg/ml, 0.46-23.96) was significantly (p<0.05) higher than that in all other groups (preterm: 0.29, 0.10-0.88; term: 0.24, 0.11-0.80; within 1 week before labor: 0.30, 0.18-0.62). Hyaluronidase activity both within 1 week before labor group (3.03 min., 1.12-3.95) and in 1st stage of labor group (3.52, 0.43-5.15) was significantly (p<0.05) higher than that in preterm group (1.70, 0.00-5.47). The molecular weight of hyaluronan in cervical mucus in the 1st stage of labor (0.97x106, 0.86-1.41) was significantly (p<0.05) lower than in the preterm and term groups (preterm: 1.60, 1.21-2.20, term: 1.41, 1.21-2.20). There was a significant correlation between hyaluronidase activity and molecular weight of hyaluronan (p<0.05, r=-0.41, n=23).


CONCLUSIONS
These findings suggest that either hyaluronidase or low-molecular weight hyaluronan could be one of the most important regulators in the process of cervical ripening.
Obara
M
M
Department of Obstetrics and Gynecology, Akita University School of Medicine, Akita, Japan.
Hirano
H
H
Ogawa
M
M
Tsubaki
H
H
Hosoya
N
N
Yoshida
Y
Y
Miyauchi
S
S
Tanaka
T
T
D000704:Analysis of Variance; D015415:Biomarkers / Q000032:analysis; D020070:Cervical Ripening / Q000378:metabolism / Q000502:physiology*; D002582:Cervix Mucus / Q000737:chemistry* / Q000201:enzymology*; D002851:Chromatography, High Pressure Liquid; D005260:Female; D005865:Gestational Age; D006801:Humans; D006820:Hyaluronic Acid / Q000032:analysis* / Q000378:metabolism; D006821:Hyaluronoglucosaminidase / Q000032:analysis* / Q000378:metabolism; D007747:Labor Stage, First; D007749:Labor Stage, Third; D008970:Molecular Weight; D011247:Pregnancy; D011336:Probability; D011446:Prospective Studies; D012680:Sensitivity and Specificity; D018709:Statistics, Nonparametric
D015415:Biomarkers; D006820:Hyaluronic Acid; D006821:Hyaluronoglucosaminidase