title: Primary structure and developmental expression of Dp ZP2, a vitelline envelope glycoprotein homolog of mouse ZP2, in Discoglossus pictus, one of the oldest living Anuran species.
abstract: A glycoprotein of the Xenopus vitelline envelope, gp 69/64, which mediates sperm binding, is closely related to the components of ZPA family, such as the mouse zona pellucida ZP2. To test the generality of these findings, we studied Discoglossus pictus, a species evolutionary distant from Xenopus and identified as a protein of 63 kDa in the vitelline envelope. Preliminary studies suggest that this protein may bind sperm at fertilization. We found that the 63-kDa protein is glycosylated and contains both N- and O-linked chains. We have cloned the cDNA encoding the Discoglossus protein of 63 kDa (Dp ZP2) by screening a Discoglossus cDNA library using Xenopus gp 69/64 cDNA as a probe. Analysis of the deduced sequence of Discoglossus protein revealed 48% identity with Xenopus gp 69/64 and 37-40% identity with mouse ZP2. The sequence conservation included a ZP domain, a potential furin cleavage site and a putative transmembrane domain. The N-terminus region of Dp ZP2 was 40% identical to the corresponding region of Xenopus gp 69/64 which has been shown to be essential for sperm binding to the VE. Although, as of yet, there is no evidence for sperm binding at the Dp ZP2 N-terminus, it is interesting that in this region three potential O-glycosylation sites are conserved in both species, in contrast to N-glycosylation sites. It was found that the Dp ZP2 mRNA is expressed in stage 1 oocytes and in the follicle cells surrounding the oocyte. Similarly, in Xenopus oocytes, the gp 69/64m RNA, was found in the oocytes, as well as in the somatic cells. Mol. Reprod. Dev. 59:133-143, 2001.
authors.affiliation: Dipartimento di Biologia Evolutiva e Comparata, Napoli, Italy.
authors.forename: M C
authors.initials: MC
authors.lastname: Vaccaro
authors.forename: M G
authors.initials: MG
authors.lastname: Santo
authors.forename: M
authors.initials: M
authors.lastname: Caputo
authors.forename: M
authors.initials: M
authors.lastname: Just
authors.forename: J D
authors.initials: JD
authors.lastname: Tian
authors.forename: H
authors.initials: H
authors.lastname: Gong
authors.forename: W J
authors.initials: WJ
authors.lastname: Lennarz
authors.forename: C
authors.initials: C
authors.lastname: Campanella
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D001001:Anura / Q000235:genetics / Q000378:metabolism* / Q000502:physiology*; D001483:Base Sequence; D003001:Cloning, Molecular; D004527:Egg Proteins / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D006031:Glycosylation; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008562:Membrane Glycoproteins / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D009865:Oocytes / Q000166:cytology / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D013084:Sperm-Ovum Interactions; D014817:Vitelline Membrane / Q000737:chemistry* / Q000378:metabolism; D014982:Xenopus laevis / Q000235:genetics / Q000378:metabolism; D000072216:Zona Pellucida Glycoproteins
chemical_list: D004527:Egg Proteins; D008562:Membrane Glycoproteins; D011956:Receptors, Cell Surface; C000606515:ZP2 protein, human; D000072216:Zona Pellucida Glycoproteins; C000606520:Zp2 protein, mouse; C035027:egg surface sperm receptor