[One simple and efficient method for purification of IgG McAb from mice ascites: caprylic acid/ammonium sulfate precipitation].
Several ascitic IgG McAbs were purificated by caprylic acid/ammonium sulfate precipitation (CA-AS), ammonium sulfate precipitation (AS) and SPA affinity chromatography. Purity of IgG purificated by CA-AS ranged from 87% to 91%, higher than that obtained by CA (65%-70%), lower than that obtained by SPA affinity chromatography (100%). The yield of IgG purificated by CA-AS ranged from 4.8 g/L to 5.5 g/L, lower than that obtained by CA (7.0-7.9 g/L), more higher than that obtained by SPA affinity chromatography (0.6 g/L). The recovery of IgG purificatied by CA-AS ranged from 39.0% to 44.4%, lower than that obtained by CA (56.0%-65.0%), more higher than that obtained by SPA affinity chromatography (7.4%). The ELISA titer of 4E2 or H6McAb was not impaired by purification of CA-AS. The above results suggested that CA-AS is one simple, efficient, rapid and low-cost method for purification of IgG McAb from ascitic fluid.
Liu
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Department of Immunology, School of Basic Medical Sciences, WCUMS, Chengdu 610041.
Cai
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Wang
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Li
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D000645:Ammonium Sulfate; D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D001201:Ascites / Q000276:immunology*; D002210:Caprylates; D011232:Chemical Precipitation; D002846:Chromatography, Affinity; D007074:Immunoglobulin G / Q000302:isolation & purification*; D051379:Mice
D000911:Antibodies, Monoclonal; D002210:Caprylates; D007074:Immunoglobulin G; C031492:octanoic acid; D000645:Ammonium Sulfate