Simultaneous measurement of antibodies to three HIV-1 antigens in newborn dried blood-spot specimens using a multiplexed microsphere-based immunoassay.
We developed a fluorescent immunoassay to simultaneously measure antibodies to three HIV-1 antigens from newborn dried blood-spot specimens. The multiplexed assay uses fluorescent microspheres and a flow analyzer. The procedure is sensitive, precise and accurate, and can be expanded to simultaneously measure additional multiple analytes from a single specimen.
Bellisario
R
R
Division of Genetic Disorders, Wadsworth Center, New York State Department of Health, 12201-0509, Albany, NY, USA. bellisar@wadsworth.org
Colinas
R J
RJ
Pass
K A
KA
D000163:Acquired Immunodeficiency Syndrome / Q000097:blood* / Q000175:diagnosis; D001800:Blood Specimen Collection / Q000379:methods; D015200:Fluoroimmunoassay / Q000379:methods*; D015483:HIV Antibodies / Q000097:blood*; D016655:HIV Core Protein p24 / Q000276:immunology*; D015699:HIV Envelope Protein gp120 / Q000276:immunology*; D019302:HIV Envelope Protein gp160 / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D007231:Infant, Newborn; D008863:Microspheres; D015997:Neonatal Screening / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D015483:HIV Antibodies; D016655:HIV Core Protein p24; D015699:HIV Envelope Protein gp120; D019302:HIV Envelope Protein gp160