Procalcitonin does not influence the surface expression of inflammatory receptors on whole blood leukocytes.
The plasma levels of procalcitonin (PCT) are increased in patients with severe bacterial infections. Its cellular origin and potential pathophysiological function in sepsis is, however, unclear. White blood cells have recently been described to express both PCT mRNA and protein. The aim of this study was to determine whether PCT has any influence on the surface expression of receptors, relevant in inflammation, on human whole blood leukocytes under normal and septic conditions. Venous blood from healthy donors was incubated with PCT (40 ng/ml or 1200 ng/ml) alone or in combination with lipopolysaccharide (LPS, 10 ng/ml) or peptidoglycan (PepG, 10 micrograms/ml) for 6 h. The surface expression of CD14, CD54, CD64, CD80, CD86 and HLA-DR was determined by flow cytometry. We could not detect any influence of PCT on the expression of these receptors. Further studies on potential effects on other cell types during infection seem warranted.
Jørgensen
P F
PF
Institute for Surgical Research, University of Oslo, The National Hospital, N-0027 Oslo, Norway. p.f.jorgensen@klinmed.uio.no
Wang
J E
JE
Solberg
R
R
Thiemermann
C
C
Foster
S J
SJ
Aasen
A O
AO
D000704:Analysis of Variance; D002116:Calcitonin / Q000494:pharmacology*; D015740:Calcitonin Gene-Related Peptide; D005434:Flow Cytometry; D006801:Humans; D007962:Leukocytes / Q000378:metabolism*; D011498:Protein Precursors / Q000494:pharmacology*; D011971:Receptors, Immunologic / Q000187:drug effects* / Q000378:metabolism*; D018805:Sepsis / Q000378:metabolism*
C000606589:CALCA protein, human; D011498:Protein Precursors; D011971:Receptors, Immunologic; D002116:Calcitonin; D015740:Calcitonin Gene-Related Peptide