Potential diagnostic test for experimental and natural ovine Taenia hydatigena cysticercosis.
An ion-exchange chromatographic fraction of Taenia hydatigena metacestode was evaluated for use in the immunodiagnosis of ovine cysticercosis. Analysis of the fraction by sodium dodecyl sulphate--polyacrylamide gel electrophoresis revealed the presence of a 68 KDa protein. Antibodies against the isolated protein were detected in 7 out of 10 experimentally infected lambs. The diagnostic potential of the 68 KDa protein was further confirmed by testing sera from naturally infected post-mortem positive (PM+) and from apparently healthy groups of animals. Eighty % and 8% of animals were found positive by enzyme-linked immunosorbent assay (ELISA) in the groups of PM+ and apparently non-infected lambs, respectively.
Panda
M R
MR
Division of Parasitology, Indian Veterinary Research Institute, Izatnagar 243 122, India.
Ghosh
S
S
Varma
T K
TK
D000818:Animals; D000909:Antibodies, Helminth / Q000032:analysis*; D000954:Antigens, Surface / Q000032:analysis / Q000276:immunology; D003551:Cysticercosis / Q000175:diagnosis / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary*; D012680:Sensitivity and Specificity; D012756:Sheep; D012757:Sheep Diseases / Q000175:diagnosis / Q000469:parasitology*; D013621:Taenia / Q000276:immunology / Q000472:pathogenicity*
C406532:4D4 antigen; D000909:Antibodies, Helminth; D000954:Antigens, Surface