title: Two consecutive zinc fingers in Sp1 and in MAZ are essential for interactions with cis-elements.
abstract: The zinc finger proteins Sp1 and Myc-associated zinc finger protein (MAZ) are transcription factors that control the expression of various genes. Regulation of transcription by these factors is based on interactions between GC-rich DNA-binding sites (GGGCGG for Sp1 and GGGAGGG for MAZ) and the carboxyl-terminal zinc finger motifs of the two proteins. Sp1 and MAZ have three and six zinc fingers, respectively, and the details of their interactions with cis-elements remain to be clarified. We demonstrate here that Sp1 and MAZ interact with the same GC-rich DNA-binding sites, apparently sharing DNA-binding sites with each other. We found that the DNA binding activities of Sp1 and MAZ depended mainly on consecutive zinc fingers, namely the second and third zinc fingers in Sp1 and the third and fourth zinc fingers in MAZ. Furthermore, the interactions of the zinc finger proteins with the same cis-elements appear to play a critical role in the regulation of gene expression. It seems plausible that two consecutive zinc finger motifs in a zinc finger protein might be essential for interaction of the protein with DNA.
authors.affiliation: RIKEN (The Institute of Physical and Chemical Research), Tsukuba Institute, Bioresource Center, 3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Song
authors.forename: H
authors.initials: H
authors.lastname: Ugai
authors.forename: K
authors.initials: K
authors.lastname: Ogawa
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.forename: A
authors.initials: A
authors.lastname: Sarai
authors.forename: Y
authors.initials: Y
authors.lastname: Obata
authors.forename: I
authors.initials: I
authors.lastname: Kanazawa
authors.forename: K
authors.initials: K
authors.lastname: Sun
authors.forename: K
authors.initials: K
authors.lastname: Itakura
authors.forename: K K
authors.initials: KK
authors.lastname: Yokoyama
mesh_terms: D016475:3T3 Cells; D020816:Amino Acid Motifs; D000818:Animals; D001665:Binding Sites; D002460:Cell Line; D004247:DNA / Q000378:metabolism; D004268:DNA-Binding Proteins; D004305:Dose-Response Relationship, Drug; D005982:Glutathione Transferase / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008958:Models, Molecular; D010957:Plasmids; D011485:Protein Binding; D016329:Sp1 Transcription Factor / Q000737:chemistry*; D014157:Transcription Factors / Q000737:chemistry*; D016335:Zinc Fingers
chemical_list: D004268:DNA-Binding Proteins; D016329:Sp1 Transcription Factor; D014157:Transcription Factors; C076035:c-MYC-associated zinc finger protein; D004247:DNA; D005982:Glutathione Transferase