Direct measurement of microkeratome gap width by electron microscopy.
OBJECTIVE
To perform an accurate direct measurement of the microkeratome gap width using scanning electron microscopy (SEM).


METHODS
Electron Microscope Unit, University of Hong Kong, Hong Kong, China.


METHODS
The Cambridge Stereoscan S440 scanning electron microscope was used to measure the gap width of 4 SCMD microkeratomes with high accuracy (+/-1.5 microm).


RESULTS
The manufacturer's gap specification for the 4 microkeratomes was 150.0 microm. The gap width measurements using SEM were 164.7 microm, 190.0 microm, 200.6 microm, and 145.9 microm and the respective errors, 9.8%, 26.7%, 33.7%, and 2.7%. Two of the 4 microkeratomes had more than a 25% error in gap width from the specification.


CONCLUSIONS
The great variation in gap width from the manufacturer's specification for the 4 SCMD microkeratomes was beyond the standard of tolerance normally accepted in laser in situ keratomileusis (LASIK). Many unexpected LASIK-related keratectasia and corneal perforations may be related to substandard microkeratome manufacturing and calibration. All new microkeratomes and blades should be validated before use to avoid keratectasia and other flap problems in LASIK.
Liu
K Y
KY
Department of Ophthalmology & Visual Sciences, The Chinese University of Hong Kong, Hong Kong Eye Hospital, Kowloon, Hong Kong, China. liukingyu@hotmail.com
Lam
D S
DS
D002138:Calibration; D003319:Corneal Stroma / Q000601:surgery; D006801:Humans; D020731:Keratomileusis, Laser In Situ / Q000295:instrumentation*; D008855:Microscopy, Electron, Scanning; D054140:Refractive Surgical Procedures; D013524:Surgical Flaps; D014894:Weights and Measures