Cloning of glycerol-3-phosphate dehydrogenase genes (ZrGPD1 and ZrGPD2) and glycerol dehydrogenase genes (ZrGCY1 and ZrGCY2) from the salt-tolerant yeast Zygosaccharomyces rouxii.
The ZrGPD1 and ZrGPD2 genes encoding putative glycerol-3-phosphate dehydrogenases were isolated from the salt-tolerant yeast, Zygosaccharomyces rouxii. Both genes are homologous to GPD1 of Saccharomyces cerevisiae and are constitutively expressed in Z. rouxii cells. Putative glycerol dehydrogenase genes, ZrGCY1 and ZrGCY2, which are highly homologous to GCY1 of S. cerevisiae, were also isolated. Since the level of transcripts of ZrGCY1 and ZrGCY2 increased in Z. rouxii cells subjected to salt stress, it is suggested that the pathway of the signal transduction of salt stress controls the expression of these genes. The Accession Nos of these sequences in GenBank are as follows: ZrGPD1, AB047394; ZrGPD2, AB047395; ZrGCY1, AB047396; ZrGCY2, AB047397.
Iwaki
T
T
Department of Biological Resources, Faculty of Agriculture, Ehime University, Matsuyama, Ehime 790-8566, Japan.
Kurono
S
S
Yokose
Y
Y
Kubota
K
K
Tamai
Y
Y
Watanabe
Y
Y
D000595:Amino Acid Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D005800:Genes, Fungal; D005993:Glycerolphosphate Dehydrogenase / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D012331:RNA, Fungal / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D015398:Signal Transduction; D012965:Sodium Chloride / Q000378:metabolism; D013401:Sugar Alcohol Dehydrogenases / Q000737:chemistry / Q000235:genetics*; D020068:Zygosaccharomyces / Q000201:enzymology / Q000235:genetics*
D012331:RNA, Fungal; D012965:Sodium Chloride; D005993:Glycerolphosphate Dehydrogenase; D013401:Sugar Alcohol Dehydrogenases; C020672:glycerol dehydrogenase