High rate of IgE-mediated histamine release from rat mesenteric mast cells.
IgE-dependent histamine release from rat mesenteric mast cells was investigated. Excised mesenterium was cut into pieces and incubated with IgE overnight at 4 degrees C for sensitization. Over 10 pieces of mesenterium specimen could be prepared from a rat. Antigen-induced histamine release from mesenterium specimen was initiated rapidly and reached a plateau in 5 min. In an optimal condition, over 50% of total histamine was released. In contrast, unpurified and purified peritoneal mast cells released only 22.5% and 5.3% of total histamine, respectively, upon IgE stimulation. Tranilast, a mast cell stabilizer, inhibited the histamine release from mesenteric mast cells significantly. The mesenterium might be useful material for studying tissue-associated mast cell activation.
Inagaki
N
N
Department of Pharmacology, Gifu Pharmaceutical University 5-6-1 Mitahorahigashi, Gifu 502-8585, Japan.
Kawasaki
H
H
Nagai
H
H
D000818:Animals; D006634:Histamine H1 Antagonists / Q000494:pharmacology; D006636:Histamine Release / Q000187:drug effects; D007073:Immunoglobulin E / Q000276:immunology*; D007700:Kinetics; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000276:immunology*; D008643:Mesentery / Q000166:cytology*; D010537:Peritoneum / Q000166:cytology; D051381:Rats; D017208:Rats, Wistar; D062367:ortho-Aminobenzoates / Q000494:pharmacology
D006634:Histamine H1 Antagonists; D062367:ortho-Aminobenzoates; D007073:Immunoglobulin E; C012293:tranilast