Vascular smooth muscle cells of recipient origin mediate intimal expansion after aortic allotransplantation in mice.
Intimal expansion by vascular smooth muscle cells (SMCs) is a characteristic feature of graft vascular disease. Whether graft intimal SMCs arise from donor or recipient tissue is not well established but has important pathogenetic implications. We examined for the presence of male cells in the expanded intima of sex-mismatched mouse aortic allografts (C57BL/6-to-BALB/c) at 30 or 60 days after transplant by in situ hybridization using a Y-chromosome probe. Study groups included male-to-female allografts, female-to-male allografts, and female-to-female allografts in recipients previously engrafted with male bone marrow. Although intimal expansion developed in all allografts, male-to-female allografts lacked Y-chromosome-positive intimal cells. In contrast, such cells were abundant in female-to-male allografts and most of these cells co-labeled for smooth muscle alpha-actin by immunostain. Female-to-female allografts in recipients with male bone marrow showed a limited number of intimal Y-chromosome-positive cells. However, none of these clearly co-labeled for smooth muscle alpha-actin and their numbers declined throughout time, consistent with graft-infiltrating inflammatory cells. We conclude that intimal expansion of mouse aortic allografts is mediated by SMCs that originated from the recipient. There was little evidence of their derivation from the bone marrow, suggesting instead the adjacent host aorta as the primary source of intimal SMCs.
Li
J
J
Vascular Biology and Transplant Immunobiology Groups, the John P. Robarts Research Institute, and The University of Western Ontario, London, Canada.
Han
X
X
Jiang
J
J
Zhong
R
R
Williams
G M
GM
Pickering
J G
JG
Chow
L H
LH
D000818:Animals; D001011:Aorta / Q000637:transplantation*; D016026:Bone Marrow Transplantation; D006083:Graft Occlusion, Vascular / Q000209:etiology* / Q000473:pathology / Q000503:physiopathology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009131:Muscle, Smooth, Vascular / Q000503:physiopathology*; D014184:Transplantation, Homologous; D014998:Y Chromosome