Human mast cell transcriptome project.
After draft reading of the human genome sequence, systemic analysis of the transcriptome (the whole transcripts present in a cell) is progressing especially in commonly available cell types. Until recently, human mast cells were not commonly available. We have succeeded to generate a substantial number of human mast cells from umbilical cord blood and from adult peripheral blood progenitors. Then, we have examined messenger RNA selectively transcribed in these mast cells using high-density oligonucleotide probe arrays. Many unexpected but important transcripts were selectively expressed in human mast cells. We discuss the results obtained from transcriptome screening by introducing our data regarding mast-cell-specific genes.
Saito
H
H
Department of Allergy and Immunology, National Children's Medical Research Center, Tokyo, Japan. hsaito@nch.go.jp
Nakajima
T
T
Matsumoto
K
K
D001773:Blood Cells / Q000276:immunology; D005312:Fetal Blood / Q000276:immunology; D020869:Gene Expression Profiling; D016045:Human Genome Project; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology; D008407:Mast Cells / Q000276:immunology / Q000502:physiology*; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014471:Umbilical Veins
D012333:RNA, Messenger