Mechanical transport and transmission of Cryptosporidium parvum oocysts by wild filth flies.
Over the course of six months wild filth flies were collected from traps left for 7-10 days in a barn with or without a calf shedding Cryptosporidium parvum Genotype 2 oocysts in diarrheic feces. The oocysts of C. parvum transported on the flies' exoskeletons and eluted from their droplets left on visited surfaces were infectious for mice. The mean number of oocysts carried by a fly varied from 4 to 131, and the total oocyst number per collection varied from 56 to approximately 4.56 x 10(3). Fly abundance and intensity of mechanical transmission of infectious C. parvum oocysts were positively correlated, and both increased significantly when an infected calf was in the barn. Molecular data showed that the oocysts shed by infected calves were carried by flies for at least 3 weeks. Filth flies can acquire infectious C. parvum oocysts from unsanitary sites, deposit them on visited surfaces, and therefore may be involved in human or animal cryptosporidiosis.
Graczyk
T K
TK
Department of Molecular Microbiology and Immunology, School of Hygiene and Public Health, Johns Hopkins University, Baltimore, Maryland 21205, USA.
Fayer
R
R
Knight
R
R
Mhangami-Ruwende
B
B
Trout
J M
JM
Da Silva
A J
AJ
Pieniazek
N J
NJ
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000635:transmission*; D003457:Cryptosporidiosis / Q000635:transmission / Q000662:veterinary*; D016785:Cryptosporidium parvum / Q000235:genetics / Q000254:growth & development*; D017931:DNA Primers; D016054:DNA, Protozoan / Q000302:isolation & purification; D004175:Diptera / Q000469:parasitology*; D006801:Humans; D007303:Insect Vectors / Q000469:parasitology*; D008297:Male; D016133:Polymerase Chain Reaction / Q000662:veterinary
D017931:DNA Primers; D016054:DNA, Protozoan