Evaluation of renal arteries with use of gadoterate meglumine-, CO(2)-, and iodixanol-enhanced DSA measurements versus histomorphometry in renal artery restenosis in rabbits.
OBJECTIVE
To experimentally evaluate gadolinium (Gd)-, carbon dioxide (CO(2))-, and iodixanol-enhanced digital subtraction angiography (DSA) versus histomorphometry in the assessment of renal artery stenosis.


METHODS
Fifteen male New Zealand White rabbits weighing 4.0 kg underwent percutaneous catheterization. Renal artery stenosis was induced by bilateral overdilation-deendothelialization (balloon diameter = 2 mm). The percentage of artery overdilation was 33%. After 4 weeks, the rabbits were randomized into two groups: group A underwent right-sided therapeutic percutaneous transluminal renal angioplasty (PTRA) (balloon diameter = 1.5 mm). After another 4 weeks, the renal arteries were evaluated by gadoterate-, iodixanol-, and CO(2)-enhanced selective quantitative DSA. The rabbits were then killed and renal arteries were perfusion-fixed for 60 minutes. Serial orcein-stained 4-um-thick slices were prepared for histomorphometry.


RESULTS
Based on morphometric data of single-stenosis versus post-PTRA restenosis lesions, no significant difference was observed between Gd- and iodixanol-enhanced quantitative DSA (r(2) > 0.95), although the iodine/Gd density ratio was equal to 3.5. Carbon dioxide less reliably allowed quantitative DSA (r(2) < 0.75).


CONCLUSIONS
Gd-based contrast agents represent a highly reliable alternative in experimental quantitative DSA evaluation of renal artery restenosis.
Le Blanche
A F
AF
Department of Radiology and Medical Imaging, Charles Foix-Jean Rostand University Hospital, Ivry-sur-Seine, France. alaine.le-blanche@cfx.ap-hop-paris.fr
Bazot
M J
MJ
Bonneau
M
M
Farres
M T
MT
Wassef
M
M
Levy
B
B
Bigot
J M
JM
Boudghene
F
F
D015901:Angiography, Digital Subtraction / Q000379:methods*; D000818:Animals; D002245:Carbon Dioxide; D003287:Contrast Media; D004195:Disease Models, Animal; D006571:Heterocyclic Compounds; D008297:Male; D008536:Meglumine; D009942:Organometallic Compounds; D011817:Rabbits; D012077:Renal Artery / Q000000981:diagnostic imaging*; D012078:Renal Artery Obstruction / Q000175:diagnosis / Q000000981:diagnostic imaging*; D014283:Triiodobenzoic Acids
D003287:Contrast Media; D006571:Heterocyclic Compounds; D009942:Organometallic Compounds; D014283:Triiodobenzoic Acids; D002245:Carbon Dioxide; D008536:Meglumine; C050823:gadolinium 1,4,7,10-tetraazacyclododecane-N,N',N'',N'''-tetraacetate; C044834:iodixanol; C072417:gadoterate meglumine