title: Ssb1 chaperone is a [PSI+] prion-curing factor.
abstract: Yeast SUP7' or SUP11 nonsense suppressors have no phenotypic expression in strains deficient in the isopentenylation of A37 in tRNA. Here we show that such strains spontaneously produce cells with a nonsense suppressor phenotype which is related to the cytoplasmically inherited determinant and manifests all the key features of the [PSI+] prion. A screen of a multicopy yeast genomic library for genes that inactivate the [PSI+]-related suppressor phenotype resulted in the isolation of the SSB1 gene. Moreover, we demonstrate that multicopy plasmid encoding the Ssb1 chaperone cures cells of the [PSI+] prion.
authors.affiliation: Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5A, 02106 Warsaw, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Chacinska
authors.forename: B
authors.initials: B
authors.lastname: Szczesniak
authors.forename: N V
authors.initials: NV
authors.lastname: Kochneva-Pervukhova
authors.forename: V V
authors.initials: VV
authors.lastname: Kushnirov
authors.forename: M D
authors.initials: MD
authors.lastname: Ter-Avanesyan
authors.forename: M
authors.initials: M
authors.lastname: Boguta
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D018389:Codon, Nonsense; D005656:Fungal Proteins / Q000235:genetics*; D005800:Genes, Fungal / Q000235:genetics; D005838:Genotype; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D018832:Molecular Chaperones / Q000235:genetics* / Q000378:metabolism; D009154:Mutation; D010641:Phenotype; D010957:Plasmids / Q000235:genetics; D011328:Prions / Q000235:genetics / Q000378:metabolism*; D012343:RNA, Transfer / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D013489:Suppression, Genetic
chemical_list: D018389:Codon, Nonsense; D005656:Fungal Proteins; D018840:HSP70 Heat-Shock Proteins; D018832:Molecular Chaperones; D011328:Prions; C095192:SSB1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D012343:RNA, Transfer