title: Lamotrigine analysis in blood and brain by high-performance liquid chromatography.
abstract: A reversed-phase high-performance liquid chromatography assay was developed and validated to determine plasma and brain lamotrigine concentrations allowing pharmacokinetic-pharmacodynamic studies of this new antiepileptic drug in patients and laboratory animals. Lamotrigine and its internal standard were extracted, under alkaline conditions, from plasma and brain homogenate, into ethyl acetate; brain proteins were previously precipitated with trichloroacetic acid. The method was linear between 0.1 and 15.0 mg/l for plasma, with a detection limit of 0.008 mg/l, and between 0.1 and 5.0 mg/l for brain homogenate, with a detection limit of 0.023 mg/l. The method proved to be simple, useful and appropriate, not only for clinical and experimental research, but also for routine monitoring of lamotrigine concentrations in patients.
authors.affiliation: Laboratory of Pharmacology, Faculty of Pharmacy, Coimbra University, Portugal.
authors.forename: M M
authors.initials: MM
authors.lastname: Castel-Branco
authors.forename: A M
authors.initials: AM
authors.lastname: Almeida
authors.forename: A C
authors.initials: AC
authors.lastname: Falcão
authors.forename: T A
authors.initials: TA
authors.lastname: Macedo
authors.forename: M M
authors.initials: MM
authors.lastname: Caramona
authors.forename: F G
authors.initials: FG
authors.lastname: Lopez
mesh_terms: D000818:Animals; D000927:Anticonvulsants / Q000032:analysis* / Q000097:blood; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004355:Drug Stability; D004827:Epilepsy / Q000097:blood; D006801:Humans; D000077213:Lamotrigine; D015394:Molecular Structure; D051381:Rats; D012680:Sensitivity and Specificity; D014227:Triazines / Q000032:analysis* / Q000097:blood / Q000592:standards
chemical_list: D000927:Anticonvulsants; D014227:Triazines; D000077213:Lamotrigine