Expression of anaphase-promoting complex 5 in balloon angioplasty-injured rat carotid arteries and mitogen-stimulated human vascular smooth muscle cells.
We utilized differential display to identify differentially expressed mRNAs induced by balloon angioplasty injury. A recently described subunit of the anaphase-promoting complex, APC5, is not expressed in uninjured rat carotid arteries. Expression of APC5 mRNA is detectable as early as 1 day post-injury, reached maximal levels by 3-7 days, and declined by 14 days post-injury. APC5 is not expressed in serum-starved cultured human vascular smooth muscle cells (VSMC), but is strongly induced by mitogenic factors, and to a lesser extent by nonmitogenic cytokines. The kinetics of APC5 expression is associated with cell cycle progression, and corresponds with PCNA expression in PDGF-stimulated human VSMC. Interestingly, APC5 is expressed in most human tissues examined, regardless of their proliferative state. These data are the first description of the cytokine-inducible expression of APC5 and suggest that expression of this gene may represent an important event in the pathogenesis of vascular proliferative diseases.
Autieri
M V
MV
Department of Physiology, Heart Failure Research Group, Temple University School of Medicine, 3420 North Broad Street, Philadelphia, Pennsylvania 19140, USA. mautieri@unix.temple.edu
D000595:Amino Acid Sequence; D064173:Anaphase-Promoting Complex-Cyclosome; D000800:Angioplasty, Balloon / Q000009:adverse effects*; D000818:Animals; D064193:Apc5 Subunit, Anaphase-Promoting Complex-Cyclosome; D020212:Carotid Artery Injuries; D017536:Carotid Artery, Common / Q000378:metabolism*; D002478:Cells, Cultured; D016207:Cytokines / Q000494:pharmacology; D018076:DNA, Complementary / Q000235:genetics; D005346:Fibroblast Growth Factors / Q000494:pharmacology; D015870:Gene Expression; D006801:Humans; D008025:Ligases / Q000235:genetics*; D008297:Male; D008934:Mitogens / Q000494:pharmacology; D008969:Molecular Sequence Data; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012689:Sequence Homology, Nucleic Acid; D043743:Ubiquitin-Protein Ligase Complexes; D044767:Ubiquitin-Protein Ligases
C577375:ANAPC5 protein, human; D064193:Apc5 Subunit, Anaphase-Promoting Complex-Cyclosome; D016207:Cytokines; D018076:DNA, Complementary; D008934:Mitogens; D010982:Platelet-Derived Growth Factor; D018809:Proliferating Cell Nuclear Antigen; D012333:RNA, Messenger; D005346:Fibroblast Growth Factors; D043743:Ubiquitin-Protein Ligase Complexes; D064173:Anaphase-Promoting Complex-Cyclosome; D044767:Ubiquitin-Protein Ligases; D008025:Ligases