title: Induction of a peroxisomal malate dehydrogenase isoform in liver of starved rats.
abstract: The influence of starvation on malate dehydrogenase (MDH) in rat liver was investigated. Native electrophoresis revealed two MDH isoforms in non-starved rats and three isoenzymes in starved rats. After sucrose density gradient centrifugation of cell organelles from liver, MDH activity was detected in the mitochondrial and cytosolic fractions from non-starved rats. However, additional activity was found in the peroxisomal fraction from starved rats. The latter was identified as the electrophoretically new isoform in starved animals. The three isoforms of malate dehydrogenase from hepatocytes were separated and partially purified by chromatography on DEAE-Toyopearl. Several kinetic and regulatory properties of the three isoforms were rather similar. It is suggested that the newly expressed isoform of MDH operates in the glyoxylate cycle of liver peroxisomes of food-starved animals.
authors.affiliation: Department of Plant Physiology and Biochemistry, Voronezh State University, Voronezh, 394693, Russia. bsbc366@main.vsu.ru
authors.forename: V N
authors.initials: VN
authors.lastname: Popov
authors.forename: S V
authors.initials: SV
authors.lastname: Volvenkin
authors.forename: T A
authors.initials: TA
authors.lastname: Kosmatykh
authors.forename: A
authors.initials: A
authors.lastname: Suad
authors.forename: C
authors.initials: C
authors.lastname: Schnarrenberger
authors.forename: A T
authors.initials: AT
authors.lastname: Eprintcev
mesh_terms: D000818:Animals; D002499:Centrifugation, Density Gradient; D002852:Chromatography, Ion Exchange; D004790:Enzyme Induction; D007527:Isoenzymes / Q000096:biosynthesis / Q000737:chemistry; D008099:Liver / Q000201:enzymology* / Q000648:ultrastructure; D008291:Malate Dehydrogenase / Q000096:biosynthesis* / Q000737:chemistry; D020675:Peroxisomes / Q000201:enzymology*; D051381:Rats; D013217:Starvation / Q000201:enzymology*; D014020:Tissue Extracts / Q000737:chemistry / Q000378:metabolism
chemical_list: D007527:Isoenzymes; D014020:Tissue Extracts; D008291:Malate Dehydrogenase