title: Effects of salmon GnRH and chicken GnRH-II on testicular apoptosis in goldfish (Carassius auratus).
abstract: Apoptosis is a form of cell death, characterized by morphological and biochemical changes. Apoptosis occurs in the normal testis and in response to different agents. In this study, we investigated the effect of gonadotropin-releasing hormone (GnRH) in testicular apoptosis in the goldfish. GnRH is a decapeptide that is expressed in different tissues, including gonads in mammalian and non-mammalian species. While GnRH is considered to be a paracrine mediator of ovarian follicular atresia, the role of GnRH in the testis is less clear. In the present study, treatments with native salmon GnRH and chicken GnRH-II increased DNA fragmentation (a hallmark of apoptosis) in the mature goldfish testis. On the other hand, gonadotropin hormone was found to act as survival factor, by decreasing spontaneous and GnRH-induced DNA fragmentation in the goldfish testis. The results demonstrate that GnRH plays an important paracrine role in the control of apoptosis in the goldfish testis.
authors.affiliation: Department of Biological Sciences, University of Calgary, T2N 1N4, Calgary, AB, Canada.
authors.forename: C V
authors.initials: CV
authors.lastname: Andreu-Vieyra
authors.forename: H R
authors.initials: HR
authors.lastname: Habibi
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002478:Cells, Cultured; D002645:Chickens; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D006054:Goldfish / Q000502:physiology*; D007987:Gonadotropin-Releasing Hormone / Q000031:analogs & derivatives* / Q000494:pharmacology*; D008297:Male; D012474:Salmon; D013737:Testis / Q000166:cytology / Q000187:drug effects*
chemical_list: D006063:Chorionic Gonadotropin; D007987:Gonadotropin-Releasing Hormone; C047717:LHRH, His(5)-Trp(7)-Tyr(8)-