2-Chloroacetaldehyde induces epsilondA DNA adducts in DNA of Raji cells as demonstrated by an improved HPLC-fluorimetry method.
Etheno adducts in DNA are promutagenic lesions and are formed from vinyl chloride, urethane, and lipid peroxidation-derived products such as 4-hydroxynonenal. Such adducts were induced in a B-lymphoid cell line (Raji) by a metabolite of vinyl chloride, 2-chloroacetaldehyde. By modification of a method of Bedell et al., etheno dA was determined in 20 microg DNA by HPLC-fluorimetry. Our method has a detection limit of 5 fmol which is one two-hundredth that of the original method. By use of this method initial evidence was found that cigarette smoke can also induce etheno dA adducts in Raji cell DNA.
Zielinski
B
B
Div. Genetic Alterations in Carcinogenesis C0700, DKFZ Heidelberg, Germany.
Hergenhahn
M
M
D000079:Acetaldehyde / Q000031:analogs & derivatives* / Q000633:toxicity*; D002273:Carcinogens / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004247:DNA / Q000187:drug effects*; D018736:DNA Adducts; D004249:DNA Damage; D006801:Humans; D015448:Leukemia, B-Cell / Q000378:metabolism*; D015227:Lipid Peroxidation / Q000187:drug effects; D010947:Plants, Toxic; D012906:Smoke / Q000009:adverse effects; D013050:Spectrometry, Fluorescence / Q000379:methods; D014026:Nicotiana / Q000009:adverse effects; D014407:Tumor Cells, Cultured / Q000187:drug effects*; D014520:Urethane / Q000633:toxicity; D014752:Vinyl Chloride / Q000633:toxicity
D002273:Carcinogens; D018736:DNA Adducts; D012906:Smoke; D014520:Urethane; D004247:DNA; C004656:chloroacetaldehyde; D000079:Acetaldehyde; D014752:Vinyl Chloride