Development and application of a high resolution liquid chromatographic method for the analysis of complex pigment distributions.
Ternary and binary gradient systems have been developed for the high-performance liquid chromatographic analysis of complex pigment distributions typical of natural samples. Improved chromatographic resolution reveals significantly more pigment components in extracts from a sediment (Priest Pot, Cumbria, UK), a microbial mat (les Salines de la Trinital, South Catalonia, Spain) and a culture (C. phaeobacteroides) including novel bacteriochlorophyll derivatives. The methods developed are directly suited to LC-MS analysis and the automated acquisition of MS/MS data for pigments.
Airs
R L
RL
Department of Chemistry, University of York, Heslington, UK.
Atkinson
J E
JE
Keely
B J
BJ
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D013058:Mass Spectrometry; D010860:Pigments, Biological / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet
D010860:Pigments, Biological