Roles of thromboxane A2 and leukotriene B4 in radicular pain induced by herniated nucleus pulposus.
Biologically active substances, such as prostaglandins, thromboxanes, and leukotrienes, which are metabolites involved in the arachidonic acid cascade, are detected in herniated disc samples obtained from patients with lumbar disc herniation. However, little is known concerning the relationships between these substances and clinical symptoms such as radicular pain. Thromboxane A2 (TXA2) induces not only potent platelet aggregation, but also blood vessel contraction. Leukotriene B4 (LTB4), a potent chemotactic agent, plays a role in inflammatory reactions by recruiting neutrophils and lymphocytes. The purpose of this study was to examine the roles of TXA2 and LTB4 in the hyperalgesia induced by application of nucleus pulposus to the lumbar nerve root in the rat. TXA2 synthetase inhibitor and LTB4 receptor antagonist, which were injected into the epidural space, decreased mechanical hyperalgesia at both three and seven days after epidural injection. There were no significant differences in sensitivity to noxious thermal stimuli following application of the nucleus pulposus or an epidural injection. Epidural injection of LTB4 receptor antagonist and/or TXA2 synthetase inhibitor may attenuate the painful radiculopathy due to lumbar disc herniation. In conclusion, our findings suggest that TXA2 and LTB4 may play significant roles in mechanical hyperalgesia induced by autologous nucleus pulposus.
Kawakami
M
M
Department of Orthopaedic Surgery, Wakayama Medical College, Wakayama City, Japan. kawakami@wakayama-med.ac.jp
Matsumoto
T
T
Tamaki
T
T
D000818:Animals; D004195:Disease Models, Animal; D004791:Enzyme Inhibitors / Q000627:therapeutic use; D006930:Hyperalgesia / Q000188:drug therapy; D007405:Intervertebral Disc Displacement / Q000150:complications / Q000473:pathology*; D007975:Leukotriene B4 / Q000502:physiology*; D008297:Male; D008689:Methacrylates / Q000627:therapeutic use; D009043:Motor Activity / Q000187:drug effects; D010666:Phenylpropionates / Q000627:therapeutic use; D051381:Rats; D017207:Rats, Sprague-Dawley; D018102:Receptors, Leukotriene B4 / Q000037:antagonists & inhibitors; D020069:Shoulder Pain / Q000188:drug therapy / Q000209:etiology / Q000473:pathology*; D013928:Thromboxane A2 / Q000502:physiology*; D013930:Thromboxane-A Synthase / Q000037:antagonists & inhibitors
D004791:Enzyme Inhibitors; D008689:Methacrylates; D010666:Phenylpropionates; D018102:Receptors, Leukotriene B4; C067523:ONO-LB 457; D007975:Leukotriene B4; D013928:Thromboxane A2; D013930:Thromboxane-A Synthase; C034364:ozagrel