title: Effects of heat pretreatment on histopathology, cytokine production, and surfactant in endotoxin-induced acute lung injury.
abstract: To determine the effect of heat stress on histopathology of acute lung injury (ALI) caused by administration of lipopolysaccharide (LPS), and to determine the roles of tumor necrosis factor (TNF)-alpha, interleukin (IL)-1beta, interferon (IFN)-gamma, IL-10 and surfactants in heat-induced tolerance to ALI, we administered either saline or LPS (3 mg/kg of body weight) intravenously to male Sprague-Dawley rats without and with heat pretreatment. Five hours after LPS or saline treatment (23 h after heat-pretreatment), samples were obtained. We found that the histopathologic features of LPS-induced ALI were attenuated by heat-pretreatment. Heat-pretreatment did not decrease the elevated plasma or BAL fluid levels of TNF-alpha, IL-1beta, and IFN-gamma by LPS. The plasma level of IL-10 in LPS-treated rats with heat-pretreatment, however, was increased compared to that of LPS-treated rats without heat-pretreatment (P = 0.001). There were no differences in the BAL fluid concentrations of light or heavy density pulmonary surfactant phospholipids depending on heat-pretreatment in LPS-treated rats. These observations suggest that IL-10 might play a role in decreasing LPS-induced acute lung injury after heat-pretreatment.
authors.affiliation: Department of Internal Medicine, Asan Medical Center, Ulsan University College of Medicine, Seoul, Korea.
authors.forename: Y
authors.initials: Y
authors.lastname: Koh
authors.forename: Y M
authors.initials: YM
authors.lastname: Lee
authors.forename: C M
authors.initials: CM
authors.lastname: Lim
authors.forename: S S
authors.initials: SS
authors.lastname: Lee
authors.forename: T S
authors.initials: TS
authors.lastname: Shim
authors.forename: S D
authors.initials: SD
authors.lastname: Lee
authors.forename: W S
authors.initials: WS
authors.lastname: Kim
authors.forename: D S
authors.initials: DS
authors.lastname: Kim
authors.forename: W D
authors.initials: WD
authors.lastname: Kim
mesh_terms: D000208:Acute Disease; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology; D016207:Cytokines / Q000096:biosynthesis*; D004731:Endotoxins / Q000633:toxicity*; D050884:HSP72 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000378:metabolism; D018869:Heat-Shock Response; D006358:Hot Temperature; D007371:Interferon-gamma / Q000096:biosynthesis; D007375:Interleukin-1 / Q000096:biosynthesis; D016753:Interleukin-10 / Q000096:biosynthesis; D008070:Lipopolysaccharides / Q000633:toxicity; D008168:Lung / Q000187:drug effects* / Q000276:immunology / Q000473:pathology; D055370:Lung Injury; D008297:Male; D011663:Pulmonary Surfactants / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
chemical_list: D016207:Cytokines; D004731:Endotoxins; D050884:HSP72 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D007375:Interleukin-1; D008070:Lipopolysaccharides; D011663:Pulmonary Surfactants; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D007371:Interferon-gamma