Evaluation of formalin-fixed paraffin-embedded tissues from vaccine site-associated sarcomas of cats for polyomavirus DNA and antigen.
OBJECTIVE
To determine whether vaccine site-associated sarcomas (VSS) from cats contain polyomavirus antigen or DNA.


METHODS
50 formalin-fixed paraffin-embedded tissue blocks of VSS from cats.


METHODS
Sections from each tissue block were evaluated for polyomavirus antigen by use of an avidin-biotin-complex immunohistochemical staining method, using rabbit anti-murine polyomavirus polyclonal antiserum as the primary antibody. The DNA was extracted from sections of each tissue block, and a polymerase chain reaction assay was performed, using primers designed to amplify regions of the bovine polyomavirus genome and consensus polyomavirus primers designed to detect unknown polyomaviruses.


RESULTS
Polyomavirus antigen and DNA were not detected in any of the VSS.


CONCLUSIONS
Results suggest that polyomaviruses likely do not have any direct involvement in the pathogenesis of VSS in cats.
Kidney
B A
BA
Department of Veterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Canada.
Haines
D M
DM
Ellis
J A
JA
Burnham
M
M
Jackson
M L
ML
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D002371:Cat Diseases / Q000276:immunology / Q000473:pathology / Q000821:virology*; D002415:Cats; D004279:DNA, Viral / Q000032:analysis*; D005354:Fibrosarcoma / Q000276:immunology / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D005557:Formaldehyde; D007150:Immunohistochemistry / Q000662:veterinary; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011120:Polyomavirus / Q000235:genetics / Q000276:immunology; D017422:Sequence Analysis, DNA; D016707:Tissue Fixation / Q000662:veterinary; D014611:Vaccination / Q000009:adverse effects / Q000662:veterinary*; D014765:Viral Vaccines / Q000009:adverse effects* / Q000235:genetics / Q000276:immunology
D000956:Antigens, Viral; D004279:DNA, Viral; D014765:Viral Vaccines; D005557:Formaldehyde