Possible role of fas antigen (CD 95) in human amniotic epithelial cell death: an in vitro study.
Treatment of human amniotic epithelial (HAE) cells with anti-Fas monoclonal antibody (CH 11) at 100 ng/ml or 1 microg/ml for 12 or 24 h increased necrotic cell death. Apoptotic cell death induced by this antibody was significantly increased, although far fewer cells underwent apoptosis, as determined by the TUNEL method. This study suggests that Fas antigen is an important mediator in HAE cell death.
Surendran
S
S
National Institute of Neuroscience, National Center of Neurology and Psychiatry, Kodaira, Tokyo, 187-8502, Japan. ssurendr@utmb.edu
D000653:Amniotic Fluid / Q000166:cytology*; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D017209:Apoptosis / Q000502:physiology*; D004847:Epithelial Cells / Q000166:cytology*; D006801:Humans; D020287:In Situ Nick-End Labeling; D066298:In Vitro Techniques; D009336:Necrosis; D010920:Placenta / Q000166:cytology*; D019014:fas Receptor / Q000276:immunology / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D019014:fas Receptor