Interleukin-6 expression by osteoblast-like MG63 cells challenged with four acrylic bone cements.
Periprosthetic osteolysis is a major clinical problem in total hip and total knee arthroplasty and polymethylmethacrylate (PMMA) is a possible etiologic factor. Recently, increasing importance was ascribed to interleukin-6 (IL-6) as an agent favouring bone resorption. The aim of the present study was to investigate the role of bone cements on IL-6 production by MG63. The effect of four acrylic bone cements (Sulfix-60, CMW 1, CMW 2, and CMW 3) on the protein release and mRNA expression of IL-6 in osteoblast-like cell line MG63 was examined using IL-1beta (0.2 microg ml(-1)) as the positive control. The extracts in minimum essential medium (MEM) of the cements were tested, following 1-h and 7-day curing. CMW 1 and CMW 2 significantly increased the IL-6 release into the culture media (p < 0.01). The cells incubated with Sulfix-60 and CMW 3 produced no significantly different levels of IL-6 than the basal production. A positive correlation was found between the concentration of IL-6 and the contents of benzoylperoxide (p = 0.0003) and barium sulphate (p < 0.0001). MG63 expressed IL-6 mRNA constitutively, as demonstrated by the positivity of the negative controls too. We conclude that CMW 1 and CMW 2 increase the production of IL-6 in MG63 cells. The response to Sulfix-60 and CMW 3 is not significantly greater than the negative control.
Cenni
E
E
Laboratorio di Fisiopatologia degli Impianti Ortopedici, Istituti Ortopedici Rizzoli, Bologna, Italy.
Granchi
D
D
Ciapetti
G
G
Stea
S
S
Savarino
L
L
Corradini
A
A
D001843:Bone Cements / Q000633:toxicity*; D001862:Bone Resorption / Q000209:etiology; D002460:Cell Line; D017077:Culture Media, Conditioned; D015870:Gene Expression / Q000187:drug effects; D006622:Hip Prosthesis; D006801:Humans; D015850:Interleukin-6 / Q000096:biosynthesis* / Q000235:genetics; D007720:Knee Prosthesis; D008422:Materials Testing; D010006:Osteoblasts / Q000187:drug effects* / Q000276:immunology* / Q000378:metabolism; D010014:Osteolysis / Q000209:etiology; D011475:Prosthesis Failure
D001843:Bone Cements; D017077:Culture Media, Conditioned; D015850:Interleukin-6