Polymerase chain reaction protocols for alpha globin haplotype polymorphisms.
Polymerase chain reaction protocols were designed specifically to amplify regions of the alpha globin complex that contain the nine common polymorphic haplotyping sites. These reactions provided a quicker and more sensitive approach to determining alpha globin haplotypes than Southern blotting methods.
Miles
K L
KL
MRC Molecular Haematology Unit, Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford, UK. kmiles@hammer.imm.ox.ac.uk
Norwich
J T
JT
Martinson
J J
JJ
Clegg
J B
JB
D001622:Betaine; D017931:DNA Primers / Q000235:genetics; D005914:Globins / Q000235:genetics*; D006239:Haplotypes; D006801:Humans; D008274:Magnesium; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D012680:Sensitivity and Specificity
D017931:DNA Primers; D001622:Betaine; D005914:Globins; D008274:Magnesium