Isolation and characterization of a veratrol:corrinoid protein methyl transferase from Acetobacterium dehalogenans.
From 3-methoxyphenol-grown cells of Acetobacterium dehalogenans, an inducible enzyme was purified that mediated the transfer of the methyl groups of veratrol (1,2-dimethoxybenzene) to a corrinoid protein enriched from the same cells. In this reaction, veratrol was converted via 2-methoxyphenol to 1,2-dihydroxybenzene. The veratrol:corrinoid protein methyl transferase, designated MTIver, had an apparent molecular mass of about 32 kDa. With respect to the N-terminal amino acid sequence and other characteristics, MTIver is different from the vanillate:corrinoid protein methyl transferase (MTIvan) isolated earlier from the same bacterium. For the methyl transfer from veratrol to tetrahydrofolate, two additional protein fractions were required, one of which contained a corrinoid protein. This protein was not identical with the corrinoid protein of the vanillate O-demethylase system. However, the latter corrinoid protein could also serve as methyl acceptor for the veratrol:corrinoid protein methyl transferase. MTIver catalyzed the demethylation of veratrol, 3,4-dimethoxybenzoate, 2-methoxyphenol, and 3-methoxyphenol. Vanillate (3-methoxy-4-hydroxybenzoate), 2-methoxybenzoate, or 4-methoxybenzoate could not serve as substrates.
Engelmann
T
T
Institut für Mikrobiologie, FSU Jena, Germany.
Kaufmann
F
F
Diekert
G
G
D000091:Acetobacter / Q000201:enzymology*; D000595:Amino Acid Sequence; D000840:Anisoles / Q000378:metabolism*; D004790:Enzyme Induction; D008745:Methylation; D008780:Methyltransferases / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D008969:Molecular Sequence Data; D010090:Oxidoreductases, O-Demethylating / Q000302:isolation & purification / Q000378:metabolism; D013379:Substrate Specificity; D014641:Vanillic Acid / Q000378:metabolism
D000840:Anisoles; C005299:veratrole; D010090:Oxidoreductases, O-Demethylating; D008780:Methyltransferases; D014641:Vanillic Acid; C094702:3-hydroxyanisole