Hepatoprotective activity of indigtone--a bioactive fraction from Indigofera tinctoria Linn.
A bioactive fraction, indigtone (FA), obtained by fractionation of a petroleum ether extract of the aerial parts of Indigofera tinctoria, showed significant dose related hepatoprotective activity against CCl(4) induced liver injury in rats and mice. Hexobarbitone induced 'sleeptime', zoxazolamine induced 'paralysis time', levels of transaminases, bilirubin and total protein in serum were employed as indices of liver injury. Pre and post treatment with FA significantly reversed the majority of the parameters altered by the hepatotoxin. This indicated the preventive and restorative effect of FA in the process of CCl(4) induced liver damage. The fraction possessed a high therapeutic ratio, as no mortality was observed up to a dose of 2 g/kg p.o. in mice.
Singh
B
B
Regional Research Laboratory, Canal Road, Jammu Tawi-180001, India.
Saxena
A K
AK
Chandan
B K
BK
Bhardwaj
V
V
Gupta
V N
VN
Suri
O P
OP
Handa
S S
SS
D000818:Animals; D002251:Carbon Tetrachloride; D056486:Chemical and Drug Induced Liver Injury; D007887:Fabaceae; D005260:Female; D022781:Hepatocytes / Q000187:drug effects*; D008107:Liver Diseases / Q000517:prevention & control*; D008297:Male; D051379:Mice; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use; D018514:Plant Structures; D010946:Plants, Medicinal; D051381:Rats
D010936:Plant Extracts; D002251:Carbon Tetrachloride