title: Differential localization of prohormone convertases PC1 and PC2 in two distinct types of secretory granules in rat pituitary gonadotrophs.
abstract: Prohormone convertases PC1 and PC2 are endoproteases involved in prohormone cleavage at pairs of basic amino acids. There is a report that prohormone convertase exists in the rat anterior pituitary gonadotrophs, where it had previously been considered that proprotein processing does not take place. In addition to luteinizing hormone and follicle-stimulating hormone, rat pituitary gonadotrophs contain chromogranin A (CgA) and secretogranin II (SgII), two members of the family of granin proteins, which have proteolytic sites in their molecules. In the present study we examined whether there is a close correlation between subcellular localization of prohormone convertases and granin proteins. Ultrathin sections of rat anterior pituitary were immunolabeled with anti-PC1 or -PC2 antisera and then stained with immunogold. Immunogold particles for PC1 were exclusively found in large, lucent secretory granules, whereas those for PC2 were seen in both large, lucent and small, dense granules. The double-immunolabeling also demonstrated colocalization of PC2 and SgII in small, dense granules and of PC1, PC2, and CgA in large, lucent granules. These immunocytochemical results suggest that PC2 may be involved in the proteolytic processing of SgII and that both PC1 and PC2 may be necessary to process CgA.
authors.affiliation: Department of Biology, Faculty of Science, Shizuoka University, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Uehara
authors.forename: Y
authors.initials: Y
authors.lastname: Yaoi
authors.forename: M
authors.initials: M
authors.lastname: Suzuki
authors.forename: K
authors.initials: K
authors.lastname: Takata
authors.forename: S
authors.initials: S
authors.lastname: Tanaka
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D016282:Aspartic Acid Endopeptidases / Q000032:analysis*; D014408:Biomarkers, Tumor / Q000032:analysis; D053379:Chromogranin A; D002864:Chromogranins / Q000032:analysis; D003594:Cytoplasmic Granules / Q000737:chemistry / Q000201:enzymology* / Q000648:ultrastructure; D019085:Fluorescent Antibody Technique, Direct; D019084:Fluorescent Antibody Technique, Indirect; D007074:Immunoglobulin G / Q000276:immunology; D007150:Immunohistochemistry; D007986:Luteinizing Hormone / Q000032:analysis / Q000737:chemistry / Q000276:immunology; D008297:Male; D016253:Microscopy, Immunoelectron; D009363:Neoplasm Proteins / Q000737:chemistry; D009479:Neuropeptides / Q000032:analysis; D010903:Pituitary Gland, Anterior / Q000166:cytology / Q000201:enzymology* / Q000378:metabolism / Q000648:ultrastructure; D045706:Proprotein Convertase 2; D043484:Proprotein Convertases; D011506:Proteins / Q000032:analysis; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar; D013381:Subtilisins / Q000032:analysis*
chemical_list: D000888:Antibodies, Anti-Idiotypic; D014408:Biomarkers, Tumor; D053379:Chromogranin A; D002864:Chromogranins; D007074:Immunoglobulin G; D009363:Neoplasm Proteins; D009479:Neuropeptides; D011506:Proteins; D007986:Luteinizing Hormone; D043484:Proprotein Convertases; D013381:Subtilisins; D045706:Proprotein Convertase 2; D016282:Aspartic Acid Endopeptidases