title: Differential inheritance modes of DNA methylation between euchromatic and heterochromatic DNA sequences in ageing fetal bovine fibroblasts.
abstract: To elucidate overall changes in DNA methylation occurring by inappropriate epigenetic control during ageing, we compared fetal bovine fibroblasts and their aged neomycin-resistant versions using bisulfite-PCR technology. Reduction in DNA methylation was observed in euchromatic repeats (18S-rRNA/art2) and promoter regions of single-copy genes (the cytokeratin/beta-lactoglobulin/interleukin-13 genes). Contrastingly, a stable maintenance of DNA methylation was revealed in various heterochromatic sequences (satellite I/II/alphoid and Bov-B). The differential inheritance mode of DNA methylation was confirmed through the analysis of individual neomycin-resistant clones. These global, multi-locus analyses provide evidence on the tendency of differential epigenetic modification between genomic DNA regions during ageing.
authors.affiliation: Animal Developmental Biotechnology Laboratory, Korea Research Institute of Bioscience and Biotechnology (KRIBB), P.O. Box 115, Yusong, Taejon 305-600, South Korea.
authors.forename: Y K
authors.initials: YK
authors.lastname: Kang
authors.forename: D B
authors.initials: DB
authors.lastname: Koo
authors.forename: J S
authors.initials: JS
authors.lastname: Park
authors.forename: Y H
authors.initials: YH
authors.lastname: Choi
authors.forename: K K
authors.initials: KK
authors.lastname: Lee
authors.forename: Y M
authors.initials: YM
authors.lastname: Han
mesh_terms: D000818:Animals; D002417:Cattle; D016922:Cellular Senescence / Q000235:genetics*; D019175:DNA Methylation; D022041:Euchromatin / Q000378:metabolism*; D005333:Fetus / Q000166:cytology; D005347:Fibroblasts / Q000502:physiology*; D006570:Heterochromatin / Q000378:metabolism*; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics
chemical_list: D022041:Euchromatin; D006570:Heterochromatin