Molecular cloning of cgrA, the gene encoding the Aspergillus nidulans ortholog of Saccharomyces cerevisiae CGR1.
Saccharomyces cerevisiae CGR1 encodes a 120-amino acid protein with a predominant nucleolar localization. In this study we report the identification and cloning of the ortholog, cgrA, from Aspergillus nidulans. The cgrA gene is comprised of three exons on A. nidulans Chromosome 7. The cDNA contains a single open reading frame (ORF) that would encode a protein of 114 amino acids with 44% sequence identity to yeast Cgr1p. A plasmid expressing cgrA complemented the impaired growth phenotype of a yeast strain that can be inducibly depleted of CGR1, and a green fluorescent protein (GFP)-tagged CgrA protein had the same nucleolar localization as the corresponding yeast protein. These results identify cgrA as the A. nidulans ortholog of yeast CGR1 and suggest evolutionary conservation of nucleolar localization mechanisms used by these proteins.
Sun
J
J
University of Cincinnati College of Medicine, Department of Pathology & Laboratory Medicine, P.O. Box 670529, Cincinnati, OH 45267-0529, USA.
Boettner
D
D
Huebner
N
N
Xu
F
F
Rhodes
J C
JC
Askew
D S
DS
D000595:Amino Acid Sequence; D001233:Aspergillus nidulans / Q000235:genetics* / Q000254:growth & development; D003001:Cloning, Molecular; D005656:Fungal Proteins / Q000235:genetics* / Q000502:physiology; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D016366:Open Reading Frames; D010957:Plasmids / Q000235:genetics; D016601:RNA-Binding Proteins; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development; D016415:Sequence Alignment
C433726:CGRA protein, Aspergillus fumigatus; D005656:Fungal Proteins; D009687:Nuclear Proteins; D016601:RNA-Binding Proteins