Modulation of the host immune system by phosphorylcholine-containing glycoproteins secreted by parasitic filarial nematodes.
Phosphorylcholine (PC) is increasingly becoming recognised as a carbohydrate-associated component of a wide variety of procaryotic and eucaryotic pathogens. Studies employing nematode PC-containing molecules indicate that it possesses a plethora of immunomodulatory activities. ES-62 is a PC-containing glycoprotein, which is secreted by the rodent filarial nematode Acanthocheilonema viteae and which provides a model system for the dissection of the mechanisms of immune evasion induced by related PC-containing glycoproteins expressed by human filarial nematodes. At concentrations equivalent to those found for PC-containing molecules in the bloodstream of parasitised humans, ES-62 is able to inhibit antigen receptor-stimulated proliferation of B and T lymphocytes in vitro and in vivo. The active component of ES-62 appears to be PC, as PC conjugated to albumin or even PC alone broadly mimic the results obtained with ES-62. PC-induced impaired lymphocyte responsiveness appears to reflect uncoupling of the antigen receptors from key intracellular proliferative signalling events such as the phosphoinositide 3-kinase, protein kinase C and Ras mitogen-activating protein kinase pathways. Although PC-ES-62 can desensitise B and T cells, not all cells are affected, and in fact it is still possible to generate an antibody response to the molecule. Dissection of this response indicates that it is of the TH-2 type. This appears to reflect the ability of ES-62 to direct the polarity of the T cell response by suppressing the production of proinflammatory cytokines, inducing the induction of anti-inflammatory cytokines and by driving the maturation of dendritic cells that direct TH-2 T cell responses.
Harnett
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Department of Immunology, University of Strathclyde, Glasgow, UK. w.harnett@strath.ac.uk
Harnett
M M
MM
D000276:Adjuvants, Immunologic / Q000032:analysis; D000818:Animals; D016207:Cytokines / Q000096:biosynthesis; D005370:Filarioidea / Q000276:immunology* / Q000472:pathogenicity; D006023:Glycoproteins / Q000737:chemistry / Q000276:immunology*; D006801:Humans; D007107:Immune System / Q000469:parasitology*; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology; D010767:Phosphorylcholine / Q000032:analysis / Q000494:pharmacology; D015398:Signal Transduction; D014329:Tropical Climate
D000276:Adjuvants, Immunologic; D016207:Cytokines; D006023:Glycoproteins; D010767:Phosphorylcholine