Identification and molecular cloning of a novel brain-specific receptor protein that binds to brain injury-derived neurotrophic peptide. Possible role for neuronal survival.
Brain injury-derived neurotrophic peptide (BINP) is a synthetic 13-mer peptide that supports neuronal survival and protects hippocampal neurons in primary cultures from cell death caused by glutamate. We have developed a monoclonal antibody named mAb 6A22 against the 40-kDa BINP-binding protein, p40BBP. mAb 6A22 inhibits binding between BINP and rat brain synaptosomes and abolishes the protective effect of BINP. The antigen of mAb 6A22 should be the BINP-binding protein that mediates the neuroprotective action of BINP. Using an expression cloning approach with mAb 6A22, we isolated a cDNA encoding a novel receptor protein that shows binding activity of BINP. COS7 cells transfected with the cloned cDNA show binding of BINP and cell surfaces that are stained by 6A22. The mRNA for p40BBP is specific for the rat brain and is increased after birth. From immunohistochemical studies using mAb 6A22, p40BBP increased after kainic acid treatment in rat hippocampal neurons.
Hama
T
T
Mitsubishi Kagaku Institute of Life Sciences, 11 Minamiooya, Machida-shi, Tokyo 194-8511, Japan. hama@libra.ls.m-kagaku.co.jp
Maruyama
M
M
Katoh-Semba
R
R
Takizawa
M
M
Iwashima
M
M
Nara
K
K
D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001483:Base Sequence; D015153:Blotting, Western; D019556:COS Cells; D002470:Cell Survival; D003001:Cloning, Molecular; D018076:DNA, Complementary; D006624:Hippocampus / Q000378:metabolism; D006801:Humans; D007106:Immune Sera; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D019169:Jurkat Cells; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics*; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D009690:Nucleic Acid Conformation; D011485:Protein Binding; D051381:Rats; D011956:Receptors, Cell Surface / Q000737:chemistry / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D014162:Transfection
D018076:DNA, Complementary; D007106:Immune Sera; D009419:Nerve Tissue Proteins; D011956:Receptors, Cell Surface; C434956:Tmem158 protein, rat; C102215:brain injury-derived neurotrophic peptide