Cloning and characterisation of tandem-repeat type galectin in rainbow trout (Oncorhynchus mykiss).
Fish beta-galactoside binding lectin (galectin) cDNA was cloned from the cDNA library of rainbow trout (Oncorhynchus mykiss) head kidney. The clone contained a single open reading frame encoding 341 amino acids (aa) (38 kDa protein), including the initiator methionine. Significant sequence homology to mammalian galectin-9 (40-55% identity) was observed. Its amino acid sequence showed two distinct N- and C-terminal domains (148 and 130 aa, respectively) connected by a peptide linker (63 aa). The galectin contains two consensus WG-E-R/K motifs thought to play an essential role in sugar-binding, indicating that this lectin is a member of the tandem-repeat type galectins which have not been identified in fish. The 1.6 kDa mRNA of the lectin was found by Northern blot analyses to be widely expressed in the spleen, head kidney, thymus, peritoneal exudate cells, ovary, gills and heart. Southern blot analyses with the probe for C-terminal of the lectin showed the existence of two hybridising genes. These results suggest that rainbow trout has at least one tandem-repeat type galectin as well as proto-type galectin.
Inagawa
H
H
Institute for Health Sciences, Tokushima Bunri University, Japan.
Kuroda
A
A
Nishizawa
T
T
Honda
T
T
Ototake
M
M
Yokomizo
U
U
Nakanishi
T
T
Soma
G
G
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern / Q000662:veterinary; D015139:Blotting, Southern / Q000662:veterinary; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics*; D037161:Galectins; D006388:Hemagglutinins / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D017686:Oncorhynchus mykiss / Q000235:genetics*; D012313:RNA / Q000737:chemistry / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D017386:Sequence Homology, Amino Acid; D020080:Tandem Repeat Sequences
D018076:DNA, Complementary; D037161:Galectins; D006388:Hemagglutinins; D012313:RNA