Novel mutations in ndh in isoniazid-resistant Mycobacterium tuberculosis isolates.
Novel mutations in NADH dehydrogenase (ndh) were detected in 8 of 84 (9.5%) isoniazid (INH)-resistant isolates (T110A [n = 1], R268H [n = 7]), but not in 22 INH-susceptible isolates of Mycobacterium tuberculosis. Significantly, all eight isolates with mutations at ndh did not have mutations at katG, kasA, or the promoter regions of inhA or ahpC, except for one isolate. Mutations in ndh appear to be an additional molecular mechanism for isoniazid resistance in M. tuberculosis.
Lee
A S
AS
Department of Clinical Research, Singapore General Hospital, 169608 Singapore.
Teo
A S
AS
Wong
S Y
SY
D000595:Amino Acid Sequence; D000995:Antitubercular Agents / Q000494:pharmacology; D004352:Drug Resistance, Microbial / Q000235:genetics; D005838:Genotype; D006801:Humans; D007538:Isoniazid / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D009154:Mutation; D009169:Mycobacterium tuberculosis / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D009245:NADH Dehydrogenase / Q000235:genetics*; D016133:Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid
D000995:Antitubercular Agents; C088908:NADH dehydrogenase II; D009245:NADH Dehydrogenase; D007538:Isoniazid