title: Differential expression of ompA, ompB, pyk, nlpD and Cpn0585 genes between normal and interferon-gamma treated cultures of Chlamydia pneumoniae.
abstract: A common feature of many chlamydial infections is that they are often asymptomatic and may persist for long periods of time if left untreated. In addition to the well recognized lytic stage of the chlamydial developmental cycle, evidence is now emerging to support a persistent phase in the cycle in which the reticulate bodies are morphologically abnormal, viable but non-infectious and presumably also have altered gene expression patterns. We used an RT-PCR approach to study the differential levels of gene transcription for 14 genes (16SrRNA, ompA, ompB, omcB, 76 kDa, gseA, pmp1, gltX, hsp60, yaeT, pyk, nlpD, Cpn0585, Cpn1046) between normal and IFN-gamma treated Chlamydia pneumoniae cell cultures. Even though the level of morphologically abnormal reticulate bodies in our IFN-gamma treated cultures was low (approximately 10% morphologically discernible, although presumably a larger percentage were in the persistent state but not yet morphologically altered) we identified five genes (ompA, ompB, pyk, nlpD, Cpn0585) that were clearly upregulated when compared to normal cultures. This gene transcript profile may be characteristic of a general stress state in Chlamydia, induced by IFN-gamma treatment in this case, but perhaps more widely induced in other in vitro and in vivo situations.
authors.affiliation: Centre for Molecular Biotechnology, School of Life Sciences, Queensland University of Technology, Brisbane, Australia.
authors.forename: S
authors.initials: S
authors.lastname: Mathews
authors.forename: C
authors.initials: C
authors.lastname: George
authors.forename: C
authors.initials: C
authors.lastname: Flegg
authors.forename: D
authors.initials: D
authors.lastname: Stenzel
authors.forename: P
authors.initials: P
authors.lastname: Timms
mesh_terms: D016993:Chlamydophila pneumoniae / Q000187:drug effects* / Q000235:genetics* / Q000648:ultrastructure; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D007371:Interferon-gamma / Q000494:pharmacology*
chemical_list: D007371:Interferon-gamma