title: Identification of choroidal ovotransferrin as a potential ocular growth regulator.
abstract: OBJECTIVE
  In an effort to identify choroidal factors potentially involved in the regulation of ocular growth, proteins released into culture medium of organ-cultured choroids were compared between control eyes and eyes recovering from form deprivation myopia.
  
  
  METHODS
  The choroids were obtained from the posterior poles of control and recovering chick eyes, and placed into organ culture containing ( 35)S-methionine/(35)S-cysteine. Culture medium was collected after 24 hours and proteins were separated and identified by SDS-polyacrylamide gel electrophoresis (SDS-PAGE), fluorography, immunoprecipitation, western blot analysis and by amino acid sequencing. Choroidal proteins were tested for their effect on scleral proteoglycan synthesis by measuring (35)SO( 4) incorporation into scleral glycosaminoglycans (GAG) in vitro. Choroidal thickness and axial elongation were measured in control and recovering eyes using high frequency A-scan ultrasound.
  
  
  RESULTS
  The synthesis of an 80 kD protein was greatly increased in the choroids of recovering eyes compared with those of control eyes. Amino acid sequencing and immunoprecipitation indicated that the newly synthesized 80 kD protein was ovotransferrin (transferrin, conalbumin). Ovotransferrin release into the culture medium by isolated recovering choroids was associated with a decrease in the rate of axial elongation in recovering eyes. When tested in vitro, ovotransferrin (500 ng/micro) inhibited scleral proteoglycan synthesis in the sclera by 62% in a dose-dependent manner.
  
  
  CONCLUSIONS
  Chick choroids of recovering eyes synthesize and release ovotransferrin during the recovery from form deprivation myopia. Ovotransferrin significantly inhibited proteoglycan synthesis by the sclera, indicating that ovotransferrin may play a role in slowing the rate of vitreous chamber elongation and facilitating the recovery from induced myopia.
authors.affiliation: Department of Anatomy & Cell Biology, School of Medicine and Health Sciences, University of North Dakota, Grand Forks, North Dakota 58202, USA. jarada@medicine.nodak.edu
authors.forename: J A
authors.initials: JA
authors.lastname: Rada
authors.forename: Y
authors.initials: Y
authors.lastname: Huang
authors.forename: K G
authors.initials: KG
authors.lastname: Rada
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D002645:Chickens; D002829:Choroid / Q000378:metabolism*; D003207:Conalbumin / Q000096:biosynthesis* / Q000494:pharmacology; D003470:Culture Media; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D005136:Eye Proteins / Q000096:biosynthesis* / Q000494:pharmacology; D006025:Glycosaminoglycans / Q000378:metabolism; D006133:Growth Substances / Q000096:biosynthesis* / Q000494:pharmacology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009216:Myopia / Q000378:metabolism*; D009924:Organ Culture Techniques; D011233:Precipitin Tests; D011509:Proteoglycans / Q000037:antagonists & inhibitors; D012590:Sclera / Q000187:drug effects; D012683:Sensory Deprivation
chemical_list: D003470:Culture Media; D005136:Eye Proteins; D006025:Glycosaminoglycans; D006133:Growth Substances; D011509:Proteoglycans; D003207:Conalbumin