Characterization of Penicillium roqueforti strains used as cheese starter cultures by RAPD typing.
Seventy-six strains of Penicillium roqueforti used as starter cultures for mould ripened blue cheeses have been analysed for their RAPD genotype by using three different primers. A comparison of the RAPD patterns within each primer group revealed that the genetic constitution of the strains was similar, as most of the strains showed very similar overall patterns. Despite these similarities with each primer, distinct RAPD genotype groups could be identified. With one of the primers, it was possible to detect two heteropolymorphic DNA regions resulting in 13 different groups. With the other two primers, three or four groups could be identified. Between the groups of the different primers marked correspondence with respect to strain distribution could be observed, indicating that the polymorphisms detected by the primers were not independent. The RAPD patterns were compared to the production of secondary metabolites. A correlation was observed between the RAPD patterns of all primers and the production of mycophenolic acid. In addition, one of the primer (ari1) was able to distinguish between P. roqueforti strains producing larger or smaller numbers of metabolites.
Geisen
R
R
Federal Research Centre for Nutrition, Institute for Hygiene and Toxicology, Karlsruhe, Germany.
Cantor
M D
MD
Hansen
T K
TK
Holzapfel
W H
WH
Jakobsen
M
M
D002611:Cheese / Q000382:microbiology*; D017931:DNA Primers; D004271:DNA, Fungal / Q000032:analysis*; D004873:Ergolines / Q000378:metabolism; D005800:Genes, Fungal; D005838:Genotype; D006576:Heterocyclic Compounds, 4 or More Rings; D007211:Indoles; D016533:Mycological Typing Techniques; D009173:Mycophenolic Acid / Q000378:metabolism; D009183:Mycotoxins / Q000378:metabolism; D009284:Naphthols / Q000378:metabolism; D010407:Penicillium / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D010879:Piperazines; D019105:Random Amplified Polymorphic DNA Technique
D017931:DNA Primers; D004271:DNA, Fungal; D004873:Ergolines; D006576:Heterocyclic Compounds, 4 or More Rings; D007211:Indoles; D009183:Mycotoxins; D009284:Naphthols; D010879:Piperazines; C012536:roquefortine; C010453:isofumigaclavine A; C015483:PR Toxin; D009173:Mycophenolic Acid