[Chemical assessment of mutagenicity of halohydrocarbons: FTIR, UV spectrum characteristics of guanine-halohydrocarbon adducts].
Determination and identification of guanine-halohydrocarbon adduct isomers were carried out by high performance liquid chromatography (HPLC) and Fourier transform infrared spectroscopy (FTIR). The experimental results showed that the UV spectral overlay of O6-guanine adducts retrieved after HPLC at 260-270 nm. The UV spectra of N-side adducts are similarly between 240-250 nm. After thin layer chromatographic separation (silica gel-GF254), the all IR spectra of the N-side adducts of guanine with different halohydrocarbons showed a absorption near 1700 cm-1 assigned the characteristic peak of C=O. The IR spectra among the adducts of O6-guanine with different halohydrocarbons are quite similar.
Xu
W
W
Research Center for Eco-Environmental Science, Chinese Academy of Sciences, Beijing 100085, China.
Tong
B
B
Huang
Q
Q
D002851:Chromatography, High Pressure Liquid; D006147:Guanine / Q000378:metabolism*; D006846:Hydrocarbons, Halogenated / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared
D006846:Hydrocarbons, Halogenated; D006147:Guanine