Suppression of Mannheimia (Pasteurella) haemolytica serovar 1 infection in lambs by intrapulmonary administration of ovine antimicrobial anionic peptide.
In this study, the efficacy of ovine antimicrobial anionic peptide (AP) was assessed in a lamb model of acute pneumonia. A single intratracheal dose of the peptide, H-DDDDDDD-OH (0.5 mg) reduced pulmonary inflammation and the concentration of Mannheimia (Pasteurella) haemolytica in infected lung tissue. Administration of H-DDDDDDD-OH after infection was more effective in reducing the consolidation and lesion scores at the deposition site than its administration prior to infection. Hence, the in vivo effectiveness of AP suggests that it may have applications in the treatment of pulmonary infections. Further studies are needed to confirm these findings and also to determine the optimal doses and intervals of H-DDDDDDD-OH therapy.
Kalfa
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VC
U.S. Department of Agriculture, Respiratory Diseases of Livestock Research Unit, National Animal Disease Center, Agricultural Research Service, P. O. Box 70, Ames, IA 50010, USA.
Palmquist
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D
Ackermann
M R
MR
Brogden
K A
KA
D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000737:chemistry; D008168:Lung / Q000473:pathology; D016978:Mannheimia haemolytica / Q000145:classification / Q000187:drug effects; D010326:Pasteurella Infections / Q000188:drug therapy / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary*; D010455:Peptides / Q000008:administration & dosage* / Q000737:chemistry; D018410:Pneumonia, Bacterial / Q000188:drug therapy / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary*; D012756:Sheep; D012757:Sheep Diseases / Q000188:drug therapy* / Q000382:microbiology / Q000473:pathology / Q000517:prevention & control; D014132:Trachea
D000900:Anti-Bacterial Agents; D010455:Peptides