Determination of loratadine in human plasma by high-performance liquid chromatographic method with ultraviolet detection.
A HPLC-UV determination of loratadine in human plasma is presented. After simple liquid-liquid extraction with 2-methylbutane-hexane (2:1) and evaporation of organic phase the compounds were re-dissolved in 0.01 M HCl, evaporated again and finally separated on a Supelcosil LC-18-DB column. The analyses were done at ambient temperature under isocratic conditions using the mobile phase: CH3CN-water-0.5 M KH2PO4-H3PO4 (440:480:80:1, v/v). UV detection was performed at 200 nm with a limit of quantification of 0.5 ng/ml. The precision was found to be satisfactory over the whole range tested (0.5-50 ng/ml) with relative standard deviations of 2.3-6.3 and 5.2-14.1% for intra- and inter-assays, respectively.
Kunicki
P K
PK
Department of Clinical Biochemistry, National Institute of Cardiology, Warsaw, Poland. biblnauk@ikard.waw.pl
D018926:Anti-Allergic Agents / Q000097:blood*; D001682:Biological Availability; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D016903:Drug Monitoring; D006634:Histamine H1 Antagonists / Q000097:blood*; D006801:Humans; D017336:Loratadine / Q000097:blood* / Q000302:isolation & purification / Q000493:pharmacokinetics; D011786:Quality Control; D015203:Reproducibility of Results
D018926:Anti-Allergic Agents; D006634:Histamine H1 Antagonists; D017336:Loratadine