Fungicidal effect of antimicrobial peptide, PMAP-23, isolated from porcine myeloid against Candida albicans.
The antifungal activity and mechanism of a 23-mer peptide, PMAP-23, derived from pig myeloid was investigated. PMAP-23 displayed strong antifungal activity against yeast and mold. To investigate the antifungal mechanism of PMAP-23, fluorescence activated flow cytometry and confocal laser scanning microscopy were performed. Candida albicans treated with PMAP-23 showed higher fluorescence intensity by propidium iodide(PI) staining, which was similar to that of Melittin than untreated cells. Confocal microscopy showed that the peptide was located in the plasma membrane. The action of peptides against fungal cell membranes was examined by treating prepared protoplasts of C. albicans with the peptide and lipid vesicle titration test. The result showed that the peptide prevented the regeneration of fungal cell walls and induced release of the fluorescent dye trapped in the artificial membrane vesicles, indicating that the peptide exerts its antifungal activity by acting on the plasma lipid membrane.
Lee
D G
DG
Research Center for Proteineous Materials, Department of Pharmacology, School of Medicine, Chosun University, 375 Seosuk-Dong, Kwangju, Dong-Ku, 501-759, Korea
Kim
D H
DH
Park
Y
Y
Kim
H K
HK
Kim
H N
HN
Shin
Y K
YK
Choi
C H
CH
Hahm
K S
KS
D000595:Amino Acid Sequence; D000818:Animals; D000935:Antifungal Agents / Q000138:chemical synthesis / Q000302:isolation & purification* / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides / Q000235:genetics / Q000302:isolation & purification* / Q000494:pharmacology*; D002176:Candida albicans / Q000187:drug effects*; D002462:Cell Membrane / Q000187:drug effects; D008081:Liposomes; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D022423:Myeloid Cells / Q000737:chemistry; D011523:Protoplasts / Q000187:drug effects; D013552:Swine
D000935:Antifungal Agents; D023181:Antimicrobial Cationic Peptides; D008081:Liposomes; C086301:porcine myeloid antibacterial peptide 23