Role of endogenous ATP at the incision area in a rat model of postoperative pain.
The aim of the present study is to characterize the role of endogenous ATP leaked from damaged cells in a rat model of postoperative pain using behavioural and immunocytochemical approaches. We found that systemic (i.v.) and local (incision area) administration of a P2 receptor antagonist, pyridoxalphosphate-6-azophenyl-2',4'-disulphonic acid (PPADS) before surgery significantly attenuated mechanical allodynia caused by an incision of the plantar surface of the hindpaw. Furthermore, PPADS significantly reduced the incision-evoked c-Fos protein expression, a marker of neuronal activity, in the dorsal horn of the spinal cord. The present findings suggest that excitatory signaling by endogenous ATP leaked from damaged cells via PPADS-sensitive P2 receptors is necessary for the induction of the postoperative pain characterized by mechanical allodynia.
Tsuda
M
M
Division of Pharmacology, National Institute of Health Sciences, Tokyo, Japan.
Koizumi
S
S
Inoue
K
K
D000255:Adenosine Triphosphate / Q000502:physiology*; D000818:Animals; D006614:Hindlimb / Q000601:surgery*; D006930:Hyperalgesia / Q000209:etiology / Q000503:physiopathology / Q000517:prevention & control; D007150:Immunohistochemistry; D008297:Male; D010149:Postoperative Pain / Q000503:physiopathology*; D011300:Preoperative Care; D016760:Proto-Oncogene Proteins c-fos / Q000037:antagonists & inhibitors; D011732:Pyridoxal Phosphate / Q000031:analogs & derivatives* / Q000627:therapeutic use; D051381:Rats; D017207:Rats, Sprague-Dawley; D014950:Wounds, Penetrating / Q000150:complications / Q000503:physiopathology*
D016760:Proto-Oncogene Proteins c-fos; C077792:pyridoxal phosphate-6-azophenyl-2',4'-disulfonic acid; D011732:Pyridoxal Phosphate; D000255:Adenosine Triphosphate