Effects of leucocyanidines on activities of metabolizing enzymes and antioxidant enzymes.
Procyanidolic oligomers (leucocyanidines, LCs) extracted from grape seeds (Vitis vinifera) are known to have antioxidant and antimutagenic activities, and a protective effect against cardiovascular disease. In the present study we examined the influence of LCs on the activities of phase 1 enzymes and conjugation enzymes and on antioxidant enzymes such as superoxide dismutase, catalase, and glutathione peroxidase. Administration of LCs (25, 50, and 100 mg/kg. p.o. for 7 d) markedly decreased the activities of NADPH-cytochrome P450 reductase, P4501A1, P4501A2, and P4503A4, but significantly increased the activities of glutathione S-transferase and phenolsulfotransferase in rat liver. However, the activities of antioxidant enzymes were not affected by LC administration. The inhibition of P450s and increases in phase II enzyme activities indicate a role for LCs as a chemopreventive agent against toxic or carcinogenic metabolites of P450 isozymes.
Seo
K
K
Toxicology Department, National Institute of Toxicological Research, Korea Food and Drug Administration, Eunpyunggu, Seoul, Korea. kwseo@kfda.go.kr
Jung
S
S
Park
M
M
Song
Y
Y
Choung
S
S
D000818:Animals; D016587:Antimutagenic Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D065607:Cytochrome P-450 Enzyme Inhibitors; D005419:Flavonoids; D005979:Glutathione Peroxidase / Q000378:metabolism; D008297:Male; D010636:Phenols / Q000494:pharmacology*; D011108:Polymers / Q000494:pharmacology*; D059808:Polyphenols; D051381:Rats; D017207:Rats, Sprague-Dawley; D019685:Rosales / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry; D013482:Superoxide Dismutase / Q000378:metabolism
D016587:Antimutagenic Agents; D000975:Antioxidants; D065607:Cytochrome P-450 Enzyme Inhibitors; D005419:Flavonoids; D010636:Phenols; D011108:Polymers; D059808:Polyphenols; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase