Production of transgenic rodents by the microinjection of cloned DNA into fertilized one-cell eggs.
Transgenic technologies that enable rapid movement between genotype and phenotype through specific loss-of-function, overexpression, or misexpression phenotypes will be crucial in the elucidation of gene sequences emerging from genome projects. This article describes detailed procedures for the generation of transgenic mice and rats by the injection of cloned DNA into the pronuclei of fertilized one-cell eggs.
Si-Hoe
S L
SL
Molecular Neuroendocrinology Research Group, Univ. Res. Centre for Neuroendocrinology, Univ. Bristol, Bristol Royal Infirmary, Marlborough Street, Bristol, BS2 8HW, UK.
Wells
S
S
Murphy
D
D
D000822:Animal Husbandry; D000827:Animal Welfare; D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics*; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary; D004247:DNA / Q000008:administration & dosage / Q000235:genetics / Q000302:isolation & purification; D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods / Q000662:veterinary; D015870:Gene Expression; D005821:Genetic Techniques; D008297:Male; D051379:Mice; D008822:Mice, Transgenic / Q000235:genetics*; D008845:Microinjections / Q000295:instrumentation; D008957:Models, Genetic; D010062:Ovulation Induction / Q000379:methods / Q000662:veterinary; D011247:Pregnancy; D051381:Rats; D014659:Vasectomy / Q000379:methods / Q000662:veterinary; D015053:Zygote
D004247:DNA