Geraniol, a component of plant essential oils, inhibits growth and polyamine biosynthesis in human colon cancer cells.
Geraniol and other monoterpenes found in essential oils of fruits and herbs have been suggested to represent a new class of agents for cancer chemoprevention. As a first step in clarifying the mode of action of geraniol on colon carcinogenesis, we studied its effects on the growth of a human colon cancer cell line (Caco-2). Geraniol (400 microM) caused a 70% inhibition of cell growth, with cells accumulating in the S transition phase of the cell cycle, and concomitant inhibition of DNA synthesis. No signs of cytotoxicity or apoptosis were detected. Geraniol caused a 50% decrease of ornithine decarboxylase activity, a key enzyme of polyamine biosynthesis, which is enhanced in cancer growth. This led to a 40% reduction of the intracellular pool of putrescine. Geraniol also activated the intracellular catabolism of polyamines, indicated by enhanced polyamine acetylation. These observations indicate that polyamine metabolism is presumably a target in the antiproliferative properties of geraniol.
Carnesecchi
S
S
Laboratory of Nutritional Chemoprevention in Digestive Oncology, Institut de Recherche contre les Cancers de l'Appareil Digestif, Strasbourg, France.
Schneider
Y
Y
Ceraline
J
J
Duranton
B
B
Gosse
F
F
Seiler
N
N
Raul
F
F
D000080462:Acyclic Monoterpenes; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D015317:Biogenic Polyamines / Q000096:biosynthesis*; D018938:Caco-2 Cells / Q000187:drug effects* / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D003110:Colonic Neoplasms / Q000188:drug therapy; D006801:Humans; D009955:Ornithine Decarboxylase / Q000096:biosynthesis / Q000187:drug effects*; D010938:Plant Oils / Q000494:pharmacology* / Q000627:therapeutic use; D013729:Terpenes / Q000494:pharmacology* / Q000627:therapeutic use
D000080462:Acyclic Monoterpenes; D015317:Biogenic Polyamines; D010938:Plant Oils; D013729:Terpenes; D009955:Ornithine Decarboxylase; C007836:geraniol