Novel method using phytase for separating soybean beta-conglycinin and glycinin.
A novel method for separating soybean beta-conglycinin and glycinin from defatted soymilk by a phytase treatment was developed. Phytase was added to defatted soymilk (1000FYT/100 g of protein) at pH 6.0, and the mixture incubated for 1 h at 40 degrees C. This procedure separated beta-conglycinin and glycinin without needing a reducing agent or cooling into the soluble and insoluble fractions, respectively. Simultaneously, most of the phytate in both proteins was removed.
Saito
T
T
New Ingredients Research Institute, Tsukuba R&D Center, Fuji Oil Co. Ltd., Tsukuba-gun, Ibaraki, Japan. saituto@fujioil.co.jp
Kohno
M
M
Tsumura
K
K
Kugimiya
W
W
Kito
M
M
D010832:6-Phytase / Q000737:chemistry*; D052179:Antigens, Plant; D004591:Electrophoresis, Polyacrylamide Gel; D005916:Globulins / Q000302:isolation & purification*; D055314:Seed Storage Proteins; D030262:Soybean Proteins; D013025:Glycine max / Q000737:chemistry*
D052179:Antigens, Plant; D005916:Globulins; D055314:Seed Storage Proteins; D030262:Soybean Proteins; C013894:beta-conglycinin protein, Glycine max; C011474:glycinin; D010832:6-Phytase