Co-existing proteins interfere with the action of nordihydroguaiaretic acid on retrograde Golgi-to-ER protein trafficking in NRK cells and alpha-glucosidase reaction in vitro.
Induction of retrograde trafficking of mannosidase II and TGN38 in NRK cells and inhibition of alpha-glucosidase in vitro by nordihydroguaiaretic acid (NDGA) were strongly interfered with by serum, serum albumin, or other unrelated proteins added to the medium or incubation mixture. These observations indicate that NDGA interacts with diverse kinds of proteins, and therefore, pharmacological effects of NDGA at cellular levels should be carefully interpreted.
Sato
K
K
Animal and Cellular Systems Laboratory, RIKEN (The Institute of Physical and Chemical Research) Hirosawa, Wako-shi, Saitama, Japan.
Muroi
M
M
Nakamura
M
M
Fujimura
M
M
Takatsuki
A
A
D000818:Animals; D002460:Cell Line; D003110:Colonic Neoplasms / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000187:drug effects / Q000378:metabolism*; D005959:Glucosidases / Q000378:metabolism*; D006056:Golgi Apparatus / Q000187:drug effects / Q000378:metabolism*; D009637:Masoprocol / Q000494:pharmacology*; D011506:Proteins / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D011506:Proteins; D009637:Masoprocol; D005959:Glucosidases