S-myotrophin promotes the hypertrophy of myotube as insulin-like growth factor-I does.
We have reported previously that a novel muscle cell growth factor, having a structure of a peptide with sugar chains, was successfully purified from porcine skeletal muscle. It was named s-myotrophin. To determine the role of s-myotrophin in skeletal muscle growth, the effect of s-myotrophin on primary cultured chick skeletal muscle cells (composed almost totally of multinucleated myotubes) was investigated by comparing s-myotrophin with Insulin-like growth factor-I (IGF-I). Both s-myotrophin and IGF-I significantly increased creatine kinase activity of the cultures; both substances gave similar responses. Intracellar protein content was also increased by the addition of these factors. The content of myosin and actin in s-myotrophin treated culture in the differentiation medium was significantly higher than that of the control (unstimulated). The content of those proteins in IGF-I treated culture was also higher than that of control, but the differences were not statistically significant. Immunoblot analysis confirmed that the amounts of myosin and actin in the myocytes were greatly increased by s-myotrophin stimulation and also by IGF-I stimulation. Morphological observations using an anti-desmin antibody staining procedure demonstrated that the size of both s-myotrophin and IGF-I treated myotubes was appreciably larger than that of control myotubes. These results suggest that s-myotrophin is a potent mediator of skeletal muscle cell hypertrophy thorough the accumulations of muscle structural proteins.
Hayashi
T
T
Laboratory of Chemistry and Technology of Animal Products, Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan.
Ogawa
T
T
Sato
M
M
Tsuchida
N
N
Fotovati
A
A
Iwamoto
H
H
Ikeuchi
Y
Y
Cassens
R G
RG
Ito
T
T
D000199:Actins / Q000378:metabolism; D000818:Animals; D002458:Cell Fractionation; D002478:Cells, Cultured; D002642:Chick Embryo; D003402:Creatine Kinase / Q000378:metabolism*; D006133:Growth Substances / Q000494:pharmacology*; D006984:Hypertrophy; D015151:Immunoblotting; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D036341:Intercellular Signaling Peptides and Proteins; D024510:Muscle Development; D018482:Muscle, Skeletal / Q000166:cytology / Q000201:enzymology / Q000254:growth & development*; D009218:Myosins / Q000378:metabolism
D000199:Actins; D006133:Growth Substances; D036341:Intercellular Signaling Peptides and Proteins; C065700:myotrophin; D007334:Insulin-Like Growth Factor I; D003402:Creatine Kinase; D009218:Myosins