Enantioselective determination of metoprolol and major metabolites in human urine by capillary electrophoresis.
The enantiomeric separation of metoprolol and its metabolites in human urine was undertaken using capillary electrophoresis (CE). Resolution of the enantiomers was achieved using carboxymethyl-beta-cyclodextrin (CM-beta-CD) as the chiral selector. A 100-mM acetate buffer (pH 4.0) containing 5% 2-propanol and 10 mM CM-beta-CD resulted in the optimum separation of the metoprolol enantiomers and its acidic metabolite in human urine. Following a single metoprolol oral administration of 100 mg racemic metoprolol tartrate, stereoselective pharmacokinetic analysis showed that urinary acidic metabolite 3 of metoprolol accounted for 62.3% of the dose with an R/S ratio of 1.23 and urinary unchanged metoprolol 1 accounted for 6.3% of the dose with an R/S ratio of 0.72.
Lim
H K
HK
College of Pharmacy, Chungnam National University, Taejon, South Korea.
Linh
P T
PT
Hong
C H
CH
Kim
K H
KH
Kang
J S
JS
D000319:Adrenergic beta-Antagonists / Q000652:urine*; D002021:Buffers; D019075:Electrophoresis, Capillary / Q000379:methods; D006801:Humans; D008790:Metoprolol / Q000378:metabolism / Q000493:pharmacokinetics / Q000652:urine*; D015394:Molecular Structure; D015203:Reproducibility of Results; D013237:Stereoisomerism
D000319:Adrenergic beta-Antagonists; D002021:Buffers; D008790:Metoprolol