title: SHIRPA, a protocol for behavioral assessment: validation for longitudinal study of neurological dysfunction in mice.
abstract: Mouse models of neurological abnormalities are only valuable if accurately assessed. The three-stage SHIRPA procedure is used for the standardised assessment of mouse phenotype and has been reported in a high throughput experiment in which different mutants were ascertained at one age point using stage 1 of the protocol. In this study we have validated SHIRPA using a large cohort with one single mutation, 'legs at odd angles that causes neurological dysfunction. The cohort aged from 1 to 16 months during this study and this is the first longitudinal SHIRPA analysis.
authors.affiliation: Neuroscience Research, SmithKline Beecham Pharmaceuticals, New Frontiers Science Park, Third Avenue, Harlow, CM19 5AW, Essex, UK. Derek_C_Rogers@sbphrd.com
authors.forename: D C
authors.initials: DC
authors.lastname: Rogers
authors.forename: J
authors.initials: J
authors.lastname: Peters
authors.forename: J E
authors.initials: JE
authors.lastname: Martin
authors.forename: S
authors.initials: S
authors.lastname: Ball
authors.forename: S J
authors.initials: SJ
authors.lastname: Nicholson
authors.forename: A S
authors.initials: AS
authors.lastname: Witherden
authors.forename: M
authors.initials: M
authors.lastname: Hafezparast
authors.forename: J
authors.initials: J
authors.lastname: Latcham
authors.forename: T L
authors.initials: TL
authors.lastname: Robinson
authors.forename: C A
authors.initials: CA
authors.lastname: Quilter
authors.forename: E M
authors.initials: EM
authors.lastname: Fisher
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000502:physiology*; D001835:Body Weight / Q000502:physiology; D002493:Central Nervous System Diseases / Q000151:congenital* / Q000175:diagnosis* / Q000503:physiopathology; D004195:Disease Models, Animal; D005260:Female; D020233:Gait Disorders, Neurologic / Q000151:congenital / Q000175:diagnosis / Q000473:pathology; D005838:Genotype; D008137:Longitudinal Studies; D008297:Male; D051379:Mice; D008818:Mice, Neurologic Mutants / Q000002:abnormalities* / Q000502:physiology; D009068:Movement / Q000502:physiology; D010641:Phenotype; D010808:Physical Examination / Q000379:methods*; D011187:Posture / Q000502:physiology; D011597:Psychomotor Performance / Q000502:physiology; D015203:Reproducibility of Results; D012727:Sex Characteristics