title: Decrease in muscarinic M2 receptors from synaptosomes in the pons and hippocampus after REM sleep deprivation in rats.
abstract: The effects of both REM sleep deprivation and its recovery on pontine and hippocampus muscarinic M2 receptors were investigated in synaptosomes using [3H]-AF-DX 384 as a ligand. Animals were divided into three groups: REM sleep deprivation group (small platforms 6.5 cm of diameter); stress group (large platforms 14 cm of diameter) and cage control group. In a second experiment REM sleep-deprived animals were allowed 48 h of recovery. REM sleep-deprived rats showed a reduction in M2 receptors compared with both intact and stress groups. Changes in M2 receptors were also observed after 48 h of recovery from REM sleep deprivation only in hippocampus. The enhancement of acetylcholine release during both REM sleep deprivation and recovery could explain the present findings.
authors.affiliation: Departamento de Fisiología, Universidad Nacional Autonoma de Mexico, México, D.F. 04510, México. salin@servidor.unam.mx
authors.forename: R J
authors.initials: RJ
authors.lastname: Salín-Pascual
authors.forename: M
authors.initials: M
authors.lastname: Díaz-Muñoz
authors.forename: L
authors.initials: L
authors.lastname: Rivera-Valerdi
authors.forename: L
authors.initials: L
authors.lastname: Ortiz-López
authors.forename: C
authors.initials: C
authors.lastname: Blanco-Centurión
mesh_terms: D000818:Animals; D006624:Hippocampus / Q000378:metabolism*; D008297:Male; D010276:Parasympatholytics / Q000378:metabolism / Q000494:pharmacology; D010890:Pirenzepine / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology; D011149:Pons / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D043585:Receptor, Muscarinic M2; D011976:Receptors, Muscarinic / Q000187:drug effects / Q000378:metabolism*; D012892:Sleep Deprivation / Q000378:metabolism; D012895:Sleep, REM / Q000502:physiology*; D013312:Stress, Physiological / Q000378:metabolism; D013574:Synaptosomes / Q000378:metabolism*
chemical_list: D010276:Parasympatholytics; D043585:Receptor, Muscarinic M2; D011976:Receptors, Muscarinic; C065959:AFDX 384; D010890:Pirenzepine