[The protective effect of thermal treatment before irradiation on CFUs from bone marrow of mice: a potential involvement of heat shock proteins].
It was studied on mice how prior whole body hyperthemia affects a colony-forming ability of bone marrow after gamma-irradiation. It was found that heating of the animals (42 degrees C, 10 min) 18-22 h before their total irradiation (4 Gy) increases 2-fold the level of CFUs8 and CFUs12 determined in the spleen exotest. The induced radioresistance correlated with accumulation of heat shock proteins, HSP70 and HSP25, in tissues of preheated mice. Injection of quercetin (a selective inhibitor of the heat shock protein synthesis) 0.5 h before the heating fully abolished both the subsequent heat shock protein accumulation and the rise in CFUs populations as compared with control. It is suggested that heat shock proteins, whose expression increases in response to hyperthermia, can play a role of endogenous radioprotectors. Possible mechanisms of their protective action under irradiation are discussed.
Maliutina
Ia V
IaV
Medical Radiological Research Center, Russian Academy of Medical Sciences, Obninsk, 249020 Russia.
Semina
O V
OV
Semenets
T N
TN
Budagova
K R
KR
Shinkarkina
A P
AP
Kabakov
A E
AE
Poverennyĭ
A M
AM
D000818:Animals; D005260:Female; D055551:HSP27 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins / Q000502:physiology; D006360:Heat-Shock Proteins; D006410:Hematopoiesis / Q000502:physiology / Q000528:radiation effects; D006412:Hematopoietic Stem Cells / Q000502:physiology / Q000528:radiation effects*; D006979:Hyperthermia, Induced; D051379:Mice; D008808:Mice, Inbred CBA; D018832:Molecular Chaperones; D009363:Neoplasm Proteins / Q000502:physiology; D051381:Rats
D055551:HSP27 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins; C486113:Hsbp1 protein, mouse; C486114:Hspb1 protein, rat; D018832:Molecular Chaperones; D009363:Neoplasm Proteins