title: The production and evaluation of antibodies for enzyme immunoassay of AZTTP.
abstract: We describe the development of the first enzyme immunoassay for quantifying AZTTP that does not use of radioactive labeling. Anti-AZTTP antibodies were raised in rabbits by immunizing with an AZTTP-kelhoyle limpet hemocyanin (KLH) conjugate. Competitive immunoassays indicated a nanomolar sensitivity to AZTTP. One of the antisera produced was specific for AZTTP.
authors.affiliation: Laboratoire de Chimie Bio-Organique, ESA CNRS 6001, Faculté des Sciences, Université de Nice-Sophia Antipolis, France.
authors.forename: F
authors.initials: F
authors.lastname: Akeb
authors.forename: C
authors.initials: C
authors.lastname: Creminon
authors.forename: J
authors.initials: J
authors.lastname: Grassi
authors.forename: R
authors.initials: R
authors.lastname: Guedj
authors.forename: D
authors.initials: D
authors.lastname: Duval
mesh_terms: D000110:Acetylcholinesterase / Q000737:chemistry; D000818:Animals; D019380:Anti-HIV Agents / Q000032:analysis* / Q000737:chemistry / Q000276:immunology*; D000906:Antibodies / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism; D002138:Calibration; D054306:Dideoxynucleotides; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006433:Hemocyanins / Q000737:chemistry / Q000276:immunology; D015394:Molecular Structure; D011817:Rabbits; D012680:Sensitivity and Specificity; D013942:Thymine Nucleotides / Q000032:analysis* / Q000737:chemistry / Q000276:immunology*; D015215:Zidovudine / Q000031:analogs & derivatives* / Q000032:analysis* / Q000737:chemistry / Q000276:immunology*
chemical_list: D019380:Anti-HIV Agents; D000906:Antibodies; D054306:Dideoxynucleotides; D013942:Thymine Nucleotides; D015215:Zidovudine; C052873:zidovudine triphosphate; D006433:Hemocyanins; D000110:Acetylcholinesterase; C032808:keyhole-limpet hemocyanin