title: Structure of the transmembrane dimer interface of glycophorin A in membrane bilayers.
abstract: The hydrophobic transmembrane domain of glycophorin A contains a sequence motif that mediates dimerization in membrane environments. Long-range interhelical distance measurements using magic angle spinning NMR spectroscopy provide high-resolution structural constraints on the packing of the dimer interface in membrane bilayers. We show that direct packing contacts occur between glycine residues at positions 79 and 83 in the transmembrane sequence. Additional interhelical constraints between Ile76 and Gly79 and between Val80 and Gly83 restrict the rotational orientation and crossing angle of the interacting helices. These results refine our previously proposed structure of the glycophorin A dimer [Smith, S. O., and Bormann, B. J. (1995) Proc. Natl. Acad. Sci. U.S.A. 92, 488-491] which revealed that the methyl groups of Val80 and Val84 are packed against Gly79 and Gly83, respectively.
authors.affiliation: Department of Biochemistry and Cell Biology, Center for Structural Biology, State University of New York at Stony Brook, 11794-5115, USA. steven.o.smith@sunysb.edu
authors.forename: S O
authors.initials: SO
authors.lastname: Smith
authors.forename: D
authors.initials: D
authors.lastname: Song
authors.forename: S
authors.initials: S
authors.lastname: Shekar
authors.forename: M
authors.initials: M
authors.lastname: Groesbeek
authors.forename: M
authors.initials: M
authors.lastname: Ziliox
authors.forename: S
authors.initials: S
authors.lastname: Aimoto
mesh_terms: D000595:Amino Acid Sequence; D019281:Dimerization; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry; D006021:Glycophorins / Q000737:chemistry*; D006801:Humans; D008051:Lipid Bilayers / Q000737:chemistry*; D008562:Membrane Glycoproteins / Q000737:chemistry*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods; D010446:Peptide Fragments / Q000737:chemistry*; D010713:Phosphatidylcholines / Q000737:chemistry; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D013499:Surface Properties
chemical_list: D006021:Glycophorins; D008051:Lipid Bilayers; D008562:Membrane Glycoproteins; D010446:Peptide Fragments; D010713:Phosphatidylcholines; C028694:1-palmitoyl-2-oleoylphosphatidylcholine; D004134:Dimyristoylphosphatidylcholine