title: Structural determination of lysophosphatidylcholines extracted from marine sponges by fast atom bombardment tandem mass spectrometry.
abstract: A series of lysophosphatidylcholines were isolated from the marine sponge Spirastrella abata by reversed-phase high performance liquid chromatography (HPLC) and analyzed by fast atom bombardment mass spectrometry (FAB-MS). Their structural elucidation was carried out with fast atom bombardment tandem mass spectrometry (FAB-MS/MS). The collision-induced dissociation (CID) of protonated and sodiated molecular ions produced diverse product ions via a series of dissociative processes. Because of the positive charge of the amine group at the end of the molecules, charge-remote fragmentation patterns of specific ions, [M + H](+) or [M + Na](+), were very helpful for the identification of product ions which are characteristic for choline and long hydrocarbon chains substituted at the glycerol back bone. Moreover, the CID-MS/MS spectra of sodium adducted molecular ions for lysophosphatidylcholines yielded common characteristic fragment ions for the choline moiety and characteristic ions [M + Na-103](+), [M + Na-85](+) and [M + Na-59](+) in the higher mass region.
authors.affiliation: Hazardous Substance Research Team, Korea Basic Science Institute, Anam 5-Ga, Sungbuk-Ku, Seoul 136-701, Korea. jongki@comp.kbsi.re.kr
authors.forename: J
authors.initials: J
authors.lastname: Hong
authors.forename: K
authors.initials: K
authors.lastname: Cho
authors.forename: Y H
authors.initials: YH
authors.lastname: Kim
authors.forename: C
authors.initials: C
authors.lastname: Cheong
authors.forename: K S
authors.initials: KS
authors.lastname: Lee
authors.forename: J H
authors.initials: JH
authors.lastname: Jung
mesh_terms: D000818:Animals; D008244:Lysophosphatidylcholines / Q000737:chemistry* / Q000302:isolation & purification; D013058:Mass Spectrometry / Q000379:methods; D015394:Molecular Structure; D011161:Porifera / Q000737:chemistry*; D012623:Seawater; D016339:Spectrometry, Mass, Fast Atom Bombardment / Q000379:methods
chemical_list: D008244:Lysophosphatidylcholines