Identification of three new DRB3* (DRB3*0106, DRB3*0107 and DRB3*02022) alleles.
Three novel DRB3* alleles were identified using CANTYPE reverse hybridization assay. The initial unusual hybridization patterns of DRB3-specific polymerase chain reaction (PCR)-amplified DNA from each subject were confirmed by cloning and sequencing analysis. DRB3*0106 allele is identical to DRB3*0101 except for a single nucleotide substitution (CTG-->GTG) changing codon 38 from Leu to Val. This polymorphism is commonly found in DRB3*03 alleles. Compared with DRB3*0202, DRB3*02022 contains a single silent nucleotide substitution (AAT-->AAC, both encoding for Asn) at codon 77. This polymorphism is also present in DRB3*0204 allele. The new DRB3*0107 allele has a sequence unique to DRB3 alleles. From codon 5 to codon 36 the sequence is identical to that of DRB3*0101 allele. From codon 37 to codon 87 the sequence of DRB1*0107 allele is identical to that of DRB3*0202. This sequence would thus explain the CANTYPE(R) DRB3-specific unusual pattern of reactions. The new DRB3*0107 could have arisen from a gene conversion between DRB3*0101 and DRB3*0202 alleles, but the DRB3*0106 and the DRB3*02022 may have been generated by a point mutation event. The DRB3*0107 allele was identified in a Caucasoid individual. The ethnic origin of the subjects carrying the other two alleles are unknown. The three alleles presented here were only identified once, in a total population of 49,000.
Tavoularis
S
S
Head Office, Canadian Blood Services, Ottawa, Ontario, Canada. sofia.tavoularis@bloodservices.ca
Ouellet
S
S
Stephens
S
S
D000483:Alleles; D001483:Base Sequence; D016026:Bone Marrow Transplantation / Q000276:immunology; D005091:Exons / Q000235:genetics / Q000276:immunology; D006684:HLA-DR Antigens / Q000235:genetics*; D059826:HLA-DRB3 Chains; D006801:Humans; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction
D006684:HLA-DR Antigens; D059826:HLA-DRB3 Chains; C553542:HLA-DRB3*01:06 antigen; C553543:HLA-DRB3*01:07 antigen; C553544:HLA-DRB3*02:02:02 antigen; D015345:Oligonucleotide Probes