Novel baculovirus expression vectors that provide sialylation of recombinant glycoproteins in lepidopteran insect cells.
This report describes novel baculovirus vectors designed to express mammalian beta1,4-galactosyltransferase and alpha2,6-sialyltransferase genes at early times after infection. Sf9 cells infected with these viral vectors, unlike cells infected with a wild-type baculovirus, produced a sialylated viral glycoprotein during the late phase of infection. Thus, the two mammalian glycosyltransferases encoded by these viral vectors are necessary and sufficient for sialylation of a foreign glycoprotein in insect cells under the conditions used in this study. While some of the new baculovirus vectors described in this study produced less, one produced wild-type levels of infectious budded virus progeny.
Jarvis
D L
DL
Department of Molecular Biology, University of Wyoming, Laramie, WY 82071-3944, USA. DLJarvis@uwyo.edu
Howe
D
D
Aumiller
J J
JJ
D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D005822:Genetic Vectors; D006023:Glycoproteins / Q000378:metabolism*; D016695:Glycosyltransferases / Q000235:genetics; D037102:Lectins / Q000378:metabolism; D019158:N-Acetylneuraminic Acid / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D018411:Spodoptera; D015678:Viral Structural Proteins / Q000378:metabolism*
D006023:Glycoproteins; D037102:Lectins; D011994:Recombinant Proteins; D015678:Viral Structural Proteins; D016695:Glycosyltransferases; D019158:N-Acetylneuraminic Acid