title: Identification of RFC(Ctf18p, Ctf8p, Dcc1p): an alternative RFC complex required for sister chromatid cohesion in S. cerevisiae.
abstract: We have identified and characterized an alternative RFC complex RFC(Ctf18p, Ctf8p, Dcc1p) that is required for sister chromatid cohesion and faithful chromosome transmission. Ctf18p, Ctf8p, and Dcc1p interact physically in a complex with Rfc2p, Rfc3p, Rfc4p, and Rfc5p but not with Rfc1p or Rad24p. Deletion of CTF18, CTF8, or DCC1 singly or in combination (ctf18Deltactf8Deltadcc1Delta) leads to sensitivity to microtubule depolymerizing drugs and a severe sister chromatid cohesion defect. Furthermore, temperature-sensitive mutations in RFC4 result in precocious sister chromatid separation. Our results highlight a novel function of the RFC proteins and support a model in which sister chromatid cohesion is established at the replication fork via a polymerase switching mechanism and a replication-coupled remodeling of chromatin.
authors.affiliation: Graduate Program in Biochemistry, Cellular and Molecular Biology, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA.
authors.forename: M L
authors.initials: ML
authors.lastname: Mayer
authors.forename: S P
authors.initials: SP
authors.lastname: Gygi
authors.forename: R
authors.initials: R
authors.lastname: Aebersold
authors.forename: P
authors.initials: P
authors.lastname: Hieter
mesh_terms: D001542:Benomyl / Q000494:pharmacology; D002453:Cell Cycle / Q000187:drug effects; D002842:Chromatids / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D004261:DNA Replication; D004268:DNA-Binding Proteins / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D017353:Gene Deletion; D018398:Homeodomain Proteins; D015778:Minor Histocompatibility Antigens; D008958:Models, Molecular; D009154:Mutation; D011233:Precipitin Tests; D011485:Protein Binding; D021122:Protein Subunits; D019253:Proto-Oncogene Proteins c-bcl-2; D051818:Replication Protein C; D012097:Repressor Proteins; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins
chemical_list: C096816:BCL2-related protein A1; C092693:CTF18 protein, S cerevisiae; D002868:Chromosomal Proteins, Non-Histone; C429768:Ctf8 protein, S cerevisiae; D004268:DNA-Binding Proteins; C429769:Dcc1 protein, S cerevisiae; D005656:Fungal Proteins; D018398:Homeodomain Proteins; C096063:MATA1 protein, S cerevisiae; D015778:Minor Histocompatibility Antigens; D021122:Protein Subunits; D019253:Proto-Oncogene Proteins c-bcl-2; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D051818:Replication Protein C; D001542:Benomyl