title: MAGE-E1, a new member of the melanoma-associated antigen gene family and its expression in human glioma.
abstract: To unearth glioma-specific genes in human glioblastoma, the serial analysis of gene expression technique was applied to a primary glioblastoma, using cultured human astrocytes as a normal control. Among the top 147 most-expressed tags in glioblastoma, we found a tag, TTTTGGGTAT, that originated from an unidentified gene and which was not detected in human astrocyte cultures. Real-time quantitative reverse transcription-PCR showed that MAGE-E1 expression was 2.6-15-fold enriched in glioblastoma relative to human astrocytes. Expressed sequence tags containing this tag were homologous to the melanoma-associated antigen gene (MAGE) family, and this new cDNA, named MAGE-E1, was cloned by the 5'-rapid amplification of cDNA ends technique. Three alternatively spliced variants (MAGE-E1a-c) were found, and deduced amino acid sequence showed that MAGE-E1a and -E1b shared the MAGE-conserved region, whereas -E1c did not. This suggests that although MAGE-E1c is expressed from one of the MAGE family, it has distinct functions from other members. Tissue distribution analysis showed that MAGE-E1 was distinct from other MAGEs. MAGE-E1 expression was detected only in brain and ovary among normal tissues. Interestingly, MAGE-E1a and/or -E1b were specifically expressed in glioma cells among cancer cells. These results indicate that MAGE-E1 is a novel and glioma-specific member of MAGE family.
authors.affiliation: National Institute for Physiological Sciences, Okazaki, 444-8585 Japan.
authors.forename: M
authors.initials: M
authors.lastname: Sasaki
authors.forename: K
authors.initials: K
authors.lastname: Nakahira
authors.forename: Y
authors.initials: Y
authors.lastname: Kawano
authors.forename: H
authors.initials: H
authors.lastname: Katakura
authors.forename: T
authors.initials: T
authors.lastname: Yoshimine
authors.forename: K
authors.initials: K
authors.lastname: Shimizu
authors.forename: S U
authors.initials: SU
authors.lastname: Kim
authors.forename: K
authors.initials: K
authors.lastname: Ikenaka
mesh_terms: D000951:Antigens, Neoplasm / Q000096:biosynthesis / Q000235:genetics*; D001253:Astrocytes / Q000276:immunology; D001483:Base Sequence; D001932:Brain Neoplasms / Q000235:genetics* / Q000276:immunology; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D005909:Glioblastoma / Q000235:genetics* / Q000276:immunology; D006801:Humans; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D015854:Up-Regulation
chemical_list: D000951:Antigens, Neoplasm; D018076:DNA, Complementary; C430404:MAGE-E1 antigen, human; D012333:RNA, Messenger