[Genetic transformation of Streptomyces globisporus strain 1912: restriction barrier and plasmid compatibility].
Low efficiency of genetic transformation of protoplasts of different strains of Streptomyces globisporus 1912 by means of DNA preparations of three vectors pIJ487, pGM160 and pWHM4 is explained by presence of the restriction barrier in the recipients. This obstacle can be overcome by the use of modified DNA of the same vectors, isolated from not numerous transformants. The frequency of transformation by such modified vector DNA was increased by two-three orders in comparison with initial DNA, isolated from Streptomyces lividans TK24, loosing restriction-modification system. The vector pCNB4001, containing the replicon of endogeneous plasmid pSG1912-1, effectively transformed the protoplasts of all S. globisporus 1912 strains. Compatibility of pIJ487 and pSG1912-1 plasmids and incompatibility of the latter and pWHM4 was shown.
Matseliukh
A B
AB
Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, Kyiv.
D004269:DNA, Bacterial; D004587:Electrophoresis, Agar Gel; D010957:Plasmids; D013302:Streptomyces / Q000235:genetics*; D014170:Transformation, Genetic
D004269:DNA, Bacterial