TerplexDNA gene carrier system targeting artery wall cells.
The development of non-viral gene carrier systems becomes more urgent and important due to the major biosafety considerations involved with application of viral vector systems for clinical gene therapy. We recently developed a novel non-viral gene carrier system, termed TerplexDNA, which showed high gene transfer efficiency when compared to the lipofectamine gene delivery system both in HepG2 and A7R5 cell lines in vitro. In present studies, we demonstrated that the TerplexDNA gene carrier system specifically delivered the reporter genes (LacZ and Luciferase) and therapeutic gene (hrVEGF(165) cDNA) into bovine aortic artery wall cells (endothelial cells and smooth muscle cells) by receptor mediated endocytosis. We found that the transfection efficiency to these primary artery wall cells, when mediated by the TerplexDNA system, was dose-dependent, saturable and was significantly inhibited by excess free LDL. The transfection efficiency of the TerplexDNA gene carrier system was approximately 60-fold higher than that of the lipofectamine gene carrier system. The TerplexDNA gene carrier system is a useful and promising tool for artery wall gene transfer.
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Department of Pharmaceutics and Pharmaceutical Chemistry, Center for Controlled Chemical Delivery, BPRB Rm. 205, University of Utah, Salt Lake City, UT 84112, USA.
Nielsen
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Han
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Wan Kim
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D000818:Animals; D001158:Arteries / Q000166:cytology / Q000187:drug effects*; D015153:Blotting, Western; D002417:Cattle; D002470:Cell Survival; D002478:Cells, Cultured; D004337:Drug Carriers / Q000008:administration & dosage*; D004586:Electrophoresis; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects; D017930:Genes, Reporter / Q000235:genetics; D006651:Histocytochemistry; D008077:Lipoproteins, LDL; D008156:Luciferases / Q000378:metabolism; D011107:Polylysine; D011108:Polymers; D013228:Stearates; D014162:Transfection / Q000379:methods*; D001616:beta-Galactosidase / Q000378:metabolism
D004337:Drug Carriers; D008077:Lipoproteins, LDL; D011108:Polymers; D013228:Stearates; C115579:stearyl-poly(lysine); D011107:Polylysine; D008156:Luciferases; D001616:beta-Galactosidase