title: Selective ex vivo expansion of cytomegalovirus-specific CD4+ and CD8+ T lymphocytes using dendritic cells pulsed with a human leucocyte antigen A*0201-restricted peptide.
abstract: Adoptive transfer of ex vivo-generated cytomegalovirus (CMV)-specific T lymphocytes may be effective in preventing CMV disease in allogeneic haematopoietic stem cell transplantation (HSCT) recipients. We developed a procedure for expansion of CMV-specific T lymphocytes based on the antigen-presenting function of donor dendritic cells (DCs), pulsed with a human leucocyte antigen A*0201-restricted pp65 nonamer peptide. CMV-specific T lymphocytes were identified following induction of interferon gamma (IFN-gamma) secretion prompted by peptide exposure. Both CD8+ and CD4+ CMV-specific T lymphocytes were selectively produced in these cultures and showed CMV-restricted cytotoxicity. The simultaneous and selective expansion of CD4+ and CD8+ CMV-specific lymphocytes might be instrumental for more efficient in vivo function of infused CMV-specific lymphocytes.
authors.affiliation: The Bone Marrow Transplant Unit, Division of Haematology, Azienda Ospedaliera Careggi, University of Florence, Florence, Italy. a.vannucchi@dfc.unifi.it
authors.forename: A M
authors.initials: AM
authors.lastname: Vannucchi
authors.forename: S
authors.initials: S
authors.lastname: Glinz
authors.forename: A
authors.initials: A
authors.lastname: Bosi
authors.forename: R
authors.initials: R
authors.lastname: Caporale
authors.forename: P
authors.initials: P
authors.lastname: Rossi-Ferrini
mesh_terms: D019264:Adoptive Transfer / Q000379:methods*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D003587:Cytomegalovirus / Q000276:immunology*; D003713:Dendritic Cells / Q000276:immunology*; D015234:HLA-A Antigens / Q000276:immunology; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D015234:HLA-A Antigens; D007371:Interferon-gamma