title: Assaying mitochondrial respiratory complex activity in mitochondria isolated from human cells and tissues.
authors.affiliation: Department of Dermatology, Medical School, University of Newcastle upon Tyne, Newcastle upon Tyne, NE2 4HH, United Kingdom.
authors.forename: M A
authors.initials: MA
authors.lastname: Birch-Machin
authors.forename: D M
authors.initials: DM
authors.lastname: Turnbull
mesh_terms: D000818:Animals; D001681:Biological Assay / Q000379:methods; D002458:Cell Fractionation; D002478:Cells, Cultured; D003902:Detergents / Q000494:pharmacology; D004579:Electron Transport / Q000502:physiology; D042967:Electron Transport Complex I; D042963:Electron Transport Complex II; D014450:Electron Transport Complex III / Q000378:metabolism*; D003576:Electron Transport Complex IV / Q000378:metabolism*; D005347:Fibroblasts / Q000737:chemistry / Q000201:enzymology / Q000378:metabolism; D005960:Glucosides / Q000494:pharmacology; D006801:Humans; D007425:Intracellular Membranes / Q000378:metabolism; D008928:Mitochondria / Q000737:chemistry / Q000201:enzymology* / Q000378:metabolism; D008931:Mitochondria, Muscle / Q000737:chemistry / Q000201:enzymology / Q000378:metabolism; D009097:Multienzyme Complexes / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000737:chemistry / Q000166:cytology / Q000201:enzymology*; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism*; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000378:metabolism*; D021122:Protein Subunits; D017494:Proton Pumps / Q000378:metabolism; D013053:Spectrophotometry / Q000379:methods; D013385:Succinate Dehydrogenase / Q000378:metabolism*; D014020:Tissue Extracts / Q000378:metabolism
chemical_list: D003902:Detergents; D005960:Glucosides; D009097:Multienzyme Complexes; D021122:Protein Subunits; D017494:Proton Pumps; D014020:Tissue Extracts; C040358:dodecyl maltoside; D010088:Oxidoreductases; D042963:Electron Transport Complex II; D013385:Succinate Dehydrogenase; D009247:NADH, NADPH Oxidoreductases; D003576:Electron Transport Complex IV; D042967:Electron Transport Complex I; D014450:Electron Transport Complex III