title: Development and application of a high resolution liquid chromatographic method for the analysis of complex pigment distributions.
abstract: Ternary and binary gradient systems have been developed for the high-performance liquid chromatographic analysis of complex pigment distributions typical of natural samples. Improved chromatographic resolution reveals significantly more pigment components in extracts from a sediment (Priest Pot, Cumbria, UK), a microbial mat (les Salines de la Trinital, South Catalonia, Spain) and a culture (C. phaeobacteroides) including novel bacteriochlorophyll derivatives. The methods developed are directly suited to LC-MS analysis and the automated acquisition of MS/MS data for pigments.
authors.affiliation: Department of Chemistry, University of York, Heslington, UK.
authors.forename: R L
authors.initials: RL
authors.lastname: Airs
authors.forename: J E
authors.initials: JE
authors.lastname: Atkinson
authors.forename: B J
authors.initials: BJ
authors.lastname: Keely
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D013058:Mass Spectrometry; D010860:Pigments, Biological / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D010860:Pigments, Biological