title: Muscular dystrophy in female dogs.
abstract: The most common form of muscular dystrophy in dogs and humans is caused by mutations in the dystrophin gene. The dystrophin gene is located on the X chromosome, and, therefore, disease-causing mutations in dystrophin occur most often in males. Therefore, females with dystrophin deficiency or other forms of muscular dystrophy may be undiagnosed or misdiagnosed. Immunohistochemistry was used to analyze dystrophin and a number of other muscle proteins associated with muscular dystrophy in humans, including sarcoglycans and laminin alpha2, in muscle biopsy specimens from 5 female dogs with pathologic changes consistent with muscular dystrophy. The female dogs were presented with a variety of clinical signs including generalized weakness, muscle wasting, tremors, exercise intolerance, gait abnormalities, and limb deformity. Serum creatine kinase activity was variably high. One dog had no detectable dystrophin in the muscle; another was mosaic, with some fibers normal and others partly dystrophin-deficient. A 3rd dog had normal dystrophin but no detectable laminin alpha2. Two dogs could not be classified. This study demonstrates the occurrence of dystrophin- and laminin alpha2-associated muscular dystrophy and the difficulty in clinical diagnosis of these disorders in female dogs.
authors.affiliation: Department of Pathology, University of California, San Diego, La Jolla 92093-0612, USA. gshelton@ucsd.edu
authors.forename: G D
authors.initials: GD
authors.lastname: Shelton
authors.forename: L A
authors.initials: LA
authors.lastname: Liu
authors.forename: L T
authors.initials: LT
authors.lastname: Guo
authors.forename: G K
authors.initials: GK
authors.lastname: Smith
authors.forename: J S
authors.initials: JS
authors.lastname: Christiansen
authors.forename: W B
authors.initials: WB
authors.lastname: Thomas
authors.forename: M O
authors.initials: MO
authors.lastname: Smith
authors.forename: K L
authors.initials: KL
authors.lastname: Kline
authors.forename: P A
authors.initials: PA
authors.lastname: March
authors.forename: T
authors.initials: T
authors.lastname: Flegel
authors.forename: E
authors.initials: E
authors.lastname: Engvall
mesh_terms: D000818:Animals; D001947:Breeding; D003402:Creatine Kinase / Q000097:blood; D004283:Dog Diseases / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D004285:Dogs; D016189:Dystrophin / Q000032:analysis* / Q000172:deficiency; D005260:Female; D007150:Immunohistochemistry; D007797:Laminin / Q000032:analysis* / Q000172:deficiency; D009137:Muscular Dystrophy, Animal / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology
chemical_list: D016189:Dystrophin; D007797:Laminin; D003402:Creatine Kinase