title: Liquid chromatography-mass spectrometry and liquid chromatography-tandem mass spectrometry determination of N-methylpyrrolidinone in riverine biofilms.
abstract: A rugged procedure utilizing reversed-phase liquid chromatography with positive-ion electrospray ionization mass spectrometry (LC-MS) along with tandem MS is described for the quantification and confirmation of N-methylpyrrolidinone (NMP) in methanolic extracts of riverine biofilm. The LC-MS method provided a 100-fold improvement in detection limits (2 ng g(-1) with a repeatability of 80-95% based on triplicate analyses) compared to a conventional LC-UV detection procedure and was applicable to quantitative analysis of biofilm samples with little or no clean up. Under low-energy collision induced dissociation (CID) conditions (17 V, laboratory frame of reference, with argon as the collision gas), two product-ions of the [M+H]+ ion were formed at m/z 69 [MH-CH3NH2]+ and m/z 58 [MH-CH3NCH]+ with relative abundances of 30% and 5%, respectively. These CID transitions were used to demonstrate that biofilm uptake of a photocatalytically-generated mixture of NMP was rapid once acclimation was achieved.
authors.affiliation: National Water Research Institute, Saskatoon, Saskatchewan, Canada. john.headley@ec.gc.ca
authors.forename: J V
authors.initials: JV
authors.lastname: Headley
authors.forename: K M
authors.initials: KM
authors.lastname: Peru
authors.forename: D A
authors.initials: DA
authors.lastname: Friesen
authors.forename: T
authors.initials: T
authors.lastname: Neu
mesh_terms: D018441:Biofilms; D019149:Bioreactors; D002853:Chromatography, Liquid / Q000379:methods*; D010084:Oxidation-Reduction; D011760:Pyrrolidinones / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D011760:Pyrrolidinones; C038678:N-methylpyrrolidone