Determination of sulfametoxazole, sulfadiazine and associated compounds in pharmaceutical preparations by capillary zone electrophoresis.
A capillary zone electrophoresis method is presented to separate sulfadiazine, sulfamethoxazole, trimethoprim, bromhexine and guaiacol by using a fused-silica capillary (60.2 cm x 75 microm I.D.). The separation was carried out at 30 kV and 25 degrees C in a 15 mM phosphate buffer adjusted to pH 6.2 as electrolyte. Under these conditions, the run time was 6 min and the limits of quantification were about 1 mg/l for every component. The method was applied to pharmaceutical preparations and the results provided recoveries close to 100%.
Berzas Nevado
J J
JJ
Departamento de Química Analítica y Tecnología de Alimentos, Facultad de Ciencias Químicas, Universidad de Castilla-La Mancha, Ciudad Real, Spain. jjberzas@qata-cr.uclm.es
Castañeda Peñalvo
G
G
Guzmán Bernardo
F J
FJ
D000890:Anti-Infective Agents / Q000032:analysis*; D002021:Buffers; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013411:Sulfadiazine / Q000032:analysis*; D013420:Sulfamethoxazole / Q000032:analysis*; D013696:Temperature
D000890:Anti-Infective Agents; D002021:Buffers; D004364:Pharmaceutical Preparations; D013411:Sulfadiazine; D013420:Sulfamethoxazole