title: Cytochemical localisation of calcium ATPase activity during the erythrocytic cell cycle of Plasmodium falciparum.
abstract: Using a cytochemical technique, we evaluated the levels of Ca(2+)-ATPase activity in the plasmatic and in the parasitophorous vacuole membrane through the different developmental stages of the Plasmodium falciparum parasitised erythrocyte. We found that the activity is detectable and remains unaltered in the plasma membrane throughout the 48 h cell cycle. However, in the parasitophorous membrane, although the activity was very similar to that measured in the plasma membrane of the young stages (younger than 20-h-old parasites), it diminished gradually with maturation and in schizonts it was almost undetectable. These data suggest that the plasma membrane Ca(2+)-ATPase is important in the maintenance of a low erythrocyte cytoplasmic Ca(2+) concentration, and that in addition it could be a way to supply the vital cation to the parasite at the beginning of the infection, when other transport mechanisms have not yet developed.
authors.affiliation: Image Analysis and Microscopy Unit, Instituto Nacional de Salud, Av. Eldorado Cra. 50, Bogota, Colombia.
authors.forename: M L
authors.initials: ML
authors.lastname: Caldas
authors.forename: M
authors.initials: M
authors.lastname: Wasserman
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D000252:Calcium-Transporting ATPases / Q000378:metabolism*; D002453:Cell Cycle; D003593:Cytoplasm / Q000378:metabolism; D004912:Erythrocytes / Q000469:parasitology*; D006651:Histocytochemistry; D006801:Humans; D010963:Plasmodium falciparum / Q000201:enzymology*
chemical_list: D000252:Calcium-Transporting ATPases; D002118:Calcium