title: Characterization of bovine atrial angiotensin-converting enzyme.
abstract: Bovine atrial angiotensin-converting enzyme (ACE) was purified to electrophoretic homogeneity. The purification procedure included ion-exchange chromatography on DEAE-Toyopearl 650M, affinity chromatography on lisinopril-agarose and gel filtration on Sephadex G-100. The bovine atrial ACE exhibited similar sensitivities to inhibition by lisinopril and captopril as lung ACE (the Ki values for the atrial and lung enzymes differed insignificantly). However, the kinetic parameters of hydrolysis of some synthetic tripeptide substrates (FA-Phe-Gly-Gly, FA-Phe-Phe-Arg, Cbz-Phe-His-Leu, Hip-His-Leu) catalyzed by bovine atrial and lung ACE varied to a greater extent. The enzymes were also characterized by some differences in activation by chloride, nitrate, and sulfate anions. These data support the hypothesis of tissue specificity of ACEs.
authors.affiliation: School of Chemistry, Lomonosov Moscow State University, Moscow, 119899, Russia.
authors.forename: E V
authors.initials: EV
authors.lastname: Garats
authors.forename: I I
authors.initials: II
authors.lastname: Nikolskaya
authors.forename: P V
authors.initials: PV
authors.lastname: Binevski
authors.forename: V F
authors.initials: VF
authors.lastname: Pozdnev
authors.forename: O A
authors.initials: OA
authors.lastname: Kost
mesh_terms: D000818:Animals; D002216:Captopril / Q000378:metabolism; D002384:Catalysis; D002417:Cattle; D002846:Chromatography, Affinity / Q000379:methods; D002850:Chromatography, Gel / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D006325:Heart Atria / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007700:Kinetics; D017706:Lisinopril / Q000378:metabolism; D008168:Lung / Q000201:enzymology*; D009842:Oligopeptides / Q000378:metabolism; D007703:Peptidyl-Dipeptidase A / Q000302:isolation & purification / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D009842:Oligopeptides; D002216:Captopril; D017706:Lisinopril; D007703:Peptidyl-Dipeptidase A