title: Analysis of the mouse Scnn1a promoter in cortical collecting duct cells and in transgenic mice.
abstract: We have isolated and characterised the promoter of the mouse Scnn1a (alpha ENaC) gene. Using transient transfections of serial deletion mutants into Scnn1a-expressing cells, we demonstrate that 1.56 kb of 5' upstream sequence is required for cell-specific expression and corticosteroid-mediated regulation. These 5' sequences are not sufficient to drive expression of a lacZ reporter gene or a rat Scnn1a cDNA in transgenic mice, where they failed to rescue Scnn1a deficiency.
authors.affiliation: Institute of Pharmacology and Toxicology, University of Lausanne, Switzerland.
authors.forename: S
authors.initials: S
authors.lastname: Kohler
authors.forename: S
authors.initials: S
authors.lastname: Pradervand
authors.forename: C
authors.initials: C
authors.lastname: Verdumo
authors.forename: A M
authors.initials: AM
authors.lastname: Mérillat
authors.forename: M
authors.initials: M
authors.lastname: Bens
authors.forename: A
authors.initials: A
authors.lastname: Vandewalle
authors.forename: F
authors.initials: F
authors.lastname: Beermann
authors.forename: E
authors.initials: E
authors.lastname: Hummler
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D016384:Consensus Sequence; D053503:Epithelial Sodium Channels; D005786:Gene Expression Regulation; D007672:Kidney Cortex / Q000378:metabolism*; D007685:Kidney Tubules, Collecting / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D015222:Sodium Channels / Q000235:genetics*; D014162:Transfection
chemical_list: D053503:Epithelial Sodium Channels; C507047:Scnn1a protein, mouse; D015222:Sodium Channels