Vitamin E protects against iron-hexachlorobenzene induced porphyria and formation of 8-hydroxydeoxyguanosine in the liver of C57BL/10ScSn mice.
The effect of vitamin E treatment on total porphyrin content, lipid peroxidation (LOOH) and 8-hydroxydeoxyguanosine (8-OHdG) was studied in the livers of C57BL/10ScSn mice following hexachlorobenzene (HCB) and iron treatment. HCB was administered i.p. (totalling 300 mg/kg) twice, with 1 week interval. Three days after the first HCB injection iron-dextran was given i.p. (500 mg Fe per kg). Vitamin E was administered weekly (20 mg/kg) by s.c. injection. Both total hepatic porphyrin and LOOH levels were significantly (P<0.001) increased in the HCB-iron treated group as compared with the control group. Mice treated additionally with vitamin E had significant (P<0.001) lower levels as compared with the HCB-iron group. Similarly, the levels of 8-OHdG were significantly (P<0.001) increased above controls after HCB-iron treatment and this increase was reduced after co-treatment with vitamin E (P<0.02). The data support the hypothesis that the mechanism of hepatic porphyrinogenicity of HCB with iron overload is an oxidative free radical process.
Horváth ME
Second Department of Medicine, Semmelweis University Medical School, Szentkriralyi u.46 1088, Budapest, Hungary.
Faux
S P
SP
Smith
A G
AG
Blázovics
A
A
van der Looij
M
M
Fehér
J
J
Cheeseman
K H
KH
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000818:Animals; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000378:metabolism*; D006581:Hexachlorobenzene / Q000633:toxicity*; D007501:Iron / Q000378:metabolism / Q000633:toxicity*; D008054:Lipid Peroxides / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D017094:Porphyrias, Hepatic / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D011166:Porphyrins / Q000378:metabolism; D014810:Vitamin E / Q000378:metabolism / Q000494:pharmacology*
D008054:Lipid Peroxides; D011166:Porphyrins; D014810:Vitamin E; D006581:Hexachlorobenzene; D000080242:8-Hydroxy-2'-Deoxyguanosine; D007501:Iron; D003849:Deoxyguanosine