[Assessment of nitric oxide level in aqueous humor under physiological conditions and after lens extraction with PMMA during experimental work in rabbits].
OBJECTIVE
To evaluate the presence of nitric oxide and measure its level in the aqueous humor of the rabbit's eye, in physiological conditions and after extracapsular lens extraction and PMMA artificial lens implantation. We also investigated nitric oxide maintenance during early postoperative period (between 1-5 day after surgery).


METHODS
We used 30 rabbits (weighing 3.0-3.5 kg) Just before surgery samples of aqueous humor were aspirated by anterior chamber puncture. Lens was extracted with extracapsular (envelope) technique. In 15 eyes PMMA IOL was implanted in the bag and 15 eyes were left aphakic. The aqueous samples were collected on 1st, 3rd, 5th days after surgery. Nitric oxide in each sample was measured with respect to fluorometric assay.


RESULTS
In aqueous humor in physiological conditions we detected nitric oxide. Its level was estimated on the value of 26.52 nM/dl. After extracapsular lens extraction in both groups the level of nitric oxide was significantly higher than in control group. The day and value of NO level was different among examined groups. Nitric oxide level diminished significantly on 5th postoperative day.


CONCLUSIONS
We came to conclusion that after ECCE and PMMA IOL implantation NO level was significantly higher as compared with control. This higher NO level after lens extraction can be responsible for the blood aqueous breakdown.
Jurowski
P
P
Kliniki Okulistycznej Instytutu Chirurgii WAM w Łodzi.
Goś
R
R
Goś
A
A
Kedziora
J
J
Błaszczyk
J
J
Piasecka
G
G
D000818:Animals; D001082:Aqueous Humor / Q000378:metabolism*; D019654:Lens Implantation, Intraocular; D009569:Nitric Oxide / Q000378:metabolism*; D019904:Polymethyl Methacrylate; D011184:Postoperative Period; D011817:Rabbits; D012016:Reference Values
D009569:Nitric Oxide; D019904:Polymethyl Methacrylate