Partial restoration of defective chloride conductance in DeltaF508 CF mice by trimethylamine oxide.
This study was designed to test the in vivo efficacy of the chemical chaperone trimethylamine oxide (TMAO) in correcting the Cl- transport defect in a mouse model of cystic fibrosis (CF). Rectal potential difference (RPD) measurements were done in matched wild-type and DeltaF508 CF mice. Mice were treated by subcutaneous injections of TMAO. Wild-type mice demonstrated a forskolin-stimulated, Cl--dependent hyperpolarization of -6.4 +/- 0.8 mV (n = 11), which was significantly increased to -13.1 +/- 1.4 mV after treatment with TMAO. DeltaF508 CF mice showed no significant responses to forskolin. Treatment with TMAO recovered a forskolin-activated RPD in DeltaF508 CF mice (-1.1 +/- 0.2 mV; n = 17) but not in CFTR null mice. The effects of TMAO were dose dependent, resulting in a slope of -0.4 +/- 0.1 mV x g(-1) x kg(-1) in DeltaF508 CF mice. The forskolin-stimulated RPD in TMAO-treated DeltaF508 CF mice was partially blocked by glibenclamide and further stimulated by apigenin. The total response to forskolin plus apigenin was -2.5 +/- 0.45 mV (n = 6 mice), corresponding to 39% of the response evoked by forskolin only in wild-type mice.
Fischer
H
H
Children's Hospital Oakland Research Institute, Oakland, California 94609, USA. hfischer@chori.org
Fukuda
N
N
Barbry
P
P
Illek
B
B
Sartori
C
C
Matthay
M A
MA
D000818:Animals; D047310:Apigenin; D018118:Chloride Channels / Q000187:drug effects* / Q000502:physiology*; D005576:Colforsin / Q000494:pharmacology; D003550:Cystic Fibrosis / Q000235:genetics* / Q000503:physiopathology*; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000235:genetics*; D004305:Dose-Response Relationship, Drug; D004553:Electric Conductivity; D004594:Electrophysiology; D005419:Flavonoids / Q000494:pharmacology; D005905:Glyburide / Q000494:pharmacology; D008744:Methylamines / Q000494:pharmacology*; D051379:Mice; D016877:Oxidants / Q000494:pharmacology*; D012007:Rectum / Q000187:drug effects / Q000503:physiopathology; D012016:Reference Values
D018118:Chloride Channels; D005419:Flavonoids; D008744:Methylamines; D016877:Oxidants; C093349:cystic fibrosis transmembrane conductance regulator delta F508; D019005:Cystic Fibrosis Transmembrane Conductance Regulator; D005576:Colforsin; D047310:Apigenin; C005855:trimethyloxamine; D005905:Glyburide