Origin of neointimal endothelium and alpha-actin-positive smooth muscle cells in transplant arteriosclerosis.
The development of transplant arteriosclerosis (TA) is today's most important problem in clinical organ transplantation. Histologically, TA is characterized by perivascular inflammation and progressive intimal thickening. Current thought on this process of vascular remodeling assumes that neointimal vascular smooth muscle (VSM) cells and endothelium in TA are graft-derived, holding that medial VSM cells proliferate and migrate into the subendothelial space in response to signals from inflammatory cells and damaged graft endothelium. Using MHC class I haplotype-specific immunohistochemical staining and single-cell PCR analyses, we show that the neointimal alpha-actin-positive VSM cells in rat aortic or cardiac allografts are of recipient and not of donor origin. In aortic but not in cardiac allografts, recipient-derived endothelial cells (ECs) replaced donor endothelium. Cyclosporine treatment prevents neointima formation and preserves the vascular media in aortic allografts. Recipient-derived ECs do not replace graft endothelium after cyclosporine treatment. We propose that, although it progresses beyond the needs of functional repair, TA reflects the activity of a normal healing process that restores vascular wall function following allograft-induced immunological injury.
Hillebrands
J L
JL
Department of Cell Biology, Immunology Section, University of Groningen, The Netherlands.
Klatter
F A
FA
van den Hurk
B M
BM
Popa
E R
ER
Nieuwenhuis
P
P
Rozing
J
J
D000199:Actins / Q000378:metabolism*; D000818:Animals; D001011:Aorta / Q000637:transplantation; D001161:Arteriosclerosis / Q000209:etiology* / Q000503:physiopathology; D016572:Cyclosporine / Q000494:pharmacology; D005260:Female; D005805:Genes, MHC Class I / Q000235:genetics; D016027:Heart Transplantation; D006801:Humans; D007150:Immunohistochemistry; D007166:Immunosuppressive Agents / Q000494:pharmacology; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D016133:Polymerase Chain Reaction; D051381:Rats; D011919:Rats, Inbred Strains; D014180:Transplantation / Q000009:adverse effects*; D017539:Tunica Intima / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*
D000199:Actins; D007166:Immunosuppressive Agents; D016572:Cyclosporine