title: Evaluation of formalin-fixed paraffin-embedded tissues from vaccine site-associated sarcomas of cats for papillomavirus DNA and antigen.
abstract: OBJECTIVE
  To determine whether vaccine site-associated sarcomas (VSS) from cats contain papillomavirus antigen or DNA.
  
  
  METHODS
  50 formalin-fixed paraffin-embedded tissue blocks of VSS from cats.
  
  
  METHODS
  Sections from each tissue block were evaluated for papillomavirus antigen by use of an avidin-biotin-complex immunohistochemical staining method, using rabbit anti-bovine papillomavirus type-1 antibody. The DNA was extracted from sections of each tissue block, and polymerase chain reaction assays were performed, using primers designed to amplify regions of the E5 gene of bovine papillomavirus and consensus primers designed to amplify a region of the L1 gene of animal papillomaviruses. Sections from 20 of the tissue blocks were evaluated by use of nonradioactive in situ hybridization for bovine papillomavirus DNA.
  
  
  RESULTS
  Papillomavirus antigen and DNA were not detected in any of the VSS.
  
  
  CONCLUSIONS
  Results suggest that papillomaviruses likely do not have any direct involvement in the pathogenesis of VSS in cats.
authors.affiliation: Department of Veterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Canada.
authors.forename: B A
authors.initials: BA
authors.lastname: Kidney
authors.forename: D M
authors.initials: DM
authors.lastname: Haines
authors.forename: J A
authors.initials: JA
authors.lastname: Ellis
authors.forename: M L
authors.initials: ML
authors.lastname: Burnham
authors.forename: J P
authors.initials: JP
authors.lastname: Teifke
authors.forename: G
authors.initials: G
authors.lastname: Czerwinski
authors.forename: M L
authors.initials: ML
authors.lastname: Jackson
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D002371:Cat Diseases / Q000473:pathology / Q000821:virology*; D002415:Cats; D004279:DNA, Viral / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D005354:Fibrosarcoma / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D007150:Immunohistochemistry / Q000662:veterinary; D017403:In Situ Hybridization / Q000662:veterinary; D027383:Papillomaviridae / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D053918:Papillomavirus Vaccines; D016133:Polymerase Chain Reaction / Q000662:veterinary; D017422:Sequence Analysis, DNA; D014611:Vaccination / Q000009:adverse effects / Q000662:veterinary*; D014765:Viral Vaccines / Q000009:adverse effects*
chemical_list: D000956:Antigens, Viral; D004279:DNA, Viral; D053918:Papillomavirus Vaccines; D014765:Viral Vaccines