title: The dispersed cell culture as model for functional studies of the subcommissural organ: preparation and characterization of the culture system.
abstract: The subcommissural organ (SCO) is an enigmatic secretory gland of the brain, which is believed to be derived from ependymal (glial) precursor cells. We here developed a dispersed cell culture system of the bovine SCO as an approach to functional analyses of this brain gland. Tissue of the bovine SCO obtained from the slaughterhouse was papain dissociated either directly after dissection or after preparation of SCO explants. The latter had been maintained for 4-6 weeks in organ culture. The dispersed cells were cultured for up to 14 days and continuously tested for their secretory state by immunostaining of their secretory product. With respect to the morphology of the SCO cells (shape, processes, nucleus), no difference was found between the culture of freshly dissociated SCOs and that of dissociated SCO explants. In all cases, the dissociation caused a dedifferentiation; typical elongated cells were formed increasingly after 1 day of culture. Thereafter, only the cellular size increased, whereas the shape and the viability of the cells remained unchanged. Proliferating SCO cells were never observed. The culture obtained from fresh SCO tissue contained more glia cells and fibrocytes than the culture prepared from SCO explants. The proliferation of glia cells and fibrocytes was suppressed by blocking the mitotic activity with cytosine-beta-D-arabino furanoside (CAF). The cytophysiological features of the cultured dispersed cells of both origins did not differ as demonstrated by classical histology, by immunocytochemistry for the secretory products of the SCO, by the characteristics of calcium influx into the cytoplasm ([Ca2+]i) and cyclic adenosine monophosphate (cAMP) after stimulation with adenosine-5-triphosphate, substance P or serotonin, and by the activation of the transcription factor cAMP-responsive element-binding protein. Because of the maintenance of their viability, their capacity to release the secretory product into the culture medium, their receptive capacity, and their signal transduction pathways, we conclude that the dispersed cell culture system, especially that obtained from SCO explants, represents an appropriate and useful model for functional studies of the mammalian SCO.
authors.affiliation: Dr Senckenbergische Anatomie Institut für Anatomie II, Klinikum der J.W. Goethe-Universität, Theodor-Stern-Kai 7, D-60590, Frankfurt, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Schöniger
authors.forename: S
authors.initials: S
authors.lastname: Wehming
authors.forename: C
authors.initials: C
authors.lastname: Gonzalez
authors.forename: K
authors.initials: K
authors.lastname: Schöbitz
authors.forename: E
authors.initials: E
authors.lastname: Rodríguez
authors.forename: A
authors.initials: A
authors.lastname: Oksche
authors.forename: C R
authors.initials: CR
authors.lastname: Yulis
authors.forename: F
authors.initials: F
authors.lastname: Nürnberger
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D012634:Bodily Secretions / Q000502:physiology*; D001973:Bromodeoxyuridine / Q000493:pharmacokinetics; D002118:Calcium / Q000378:metabolism; D002417:Cattle; D015816:Cell Adhesion Molecules, Neuronal / Q000276:immunology / Q000378:metabolism; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D048429:Cell Size / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000096:biosynthesis; D017362:Cyclic AMP Response Element-Binding Protein / Q000187:drug effects / Q000378:metabolism; D007150:Immunohistochemistry; D009457:Neuroglia / Q000166:cytology / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D013351:Subcommissural Organ / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism
chemical_list: D015816:Cell Adhesion Molecules, Neuronal; D017362:Cyclic AMP Response Element-Binding Protein; C108402:SCO-spondin; D005576:Colforsin; D000255:Adenosine Triphosphate; D000242:Cyclic AMP; D001973:Bromodeoxyuridine; D002118:Calcium