title: Recombinant Microdochium nivale carbohydrate oxidase and its application in an amperometric glucose sensor.
abstract: Biosensors containing recombinant carbohydrate oxidase from Microdochium nivale (rMnO) were developed by means of either chemically modified carbon paste or graphite electrode. 1-(N,N-dimethylamine)-4-(4-morpholine)benzene (AMB) and 1,1'-dimethylferrocene (DMFc) have been used as mediators. The biosensors showed a linear calibration graph up to 18 mM of glucose when operated at 0.04-0.36 V versus a saturated calomel electrode. Almost no change was detected in the sensitivity of the biosensors at pH 7.2-8.1. The biosensors responded to other aldoses in the D-configuration, however, maximal sensitivity of the biosensor was towards D-glucose. The biosensor did not response to polyhydroxylic compounds such as D-mannitol, D-sorbitol and inositol. The advantages of the biosensors based on rMnO in comparison to Aspergillus niger glucose oxidase is a wider linear range, low sensitivity to oxygen and (in some cases) broad specificity.
authors.affiliation: Institute of Biochemistry, Mokslininku 12, 2600 Vilnius, Lithuania. jkulys@bchi.lt
authors.forename: J
authors.initials: J
authors.lastname: Kulys
authors.forename: L
authors.initials: L
authors.lastname: Tetianec
authors.forename: P
authors.initials: P
authors.lastname: Schneider
mesh_terms: D001234:Aspergillus niger; D015374:Biosensing Techniques; D002237:Carbohydrate Dehydrogenases / Q000737:chemistry / Q000235:genetics; D005947:Glucose / Q000032:analysis*; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D011994:Recombinant Proteins; D002237:Carbohydrate Dehydrogenases; D005947:Glucose