title: The replacement H3.3 histone gene in Paracentrotus lividus sea urchin: structure and regulatory elements.
abstract: We have isolated the Paracentrotus lividus sea urchin H3.3 histone gene and characterized the nucleotide sequences of the gene and its proximal promoter. Band shift experiments showed that two cAMP/PMA responsive elements (CRE/TRE), present in the proximal promoter, bind nuclear factors present in embryos at the blastula and gastrula stages (CRE1) and at the blastula stage (CRE2). The putative H3.3 coding region activating sequences (CRAS) failed to bind nuclear factors while the corresponding elements of the two replication-dependent genes (H3L and late H3) clearly recognized nuclear proteins. These results suggest some role of the CRE/TRE elements but not CRAS elements in the transcriptional regulation of the replication-independent histone genes in invertebrates.
authors.affiliation: Department of Genetics, General and Molecular Biology, University of Naples Frederico II, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Mancini
authors.forename: M
authors.initials: M
authors.lastname: Dentice
authors.forename: F
authors.initials: F
authors.lastname: Aniello
authors.forename: M
authors.initials: M
authors.lastname: Branno
authors.forename: M
authors.initials: M
authors.lastname: Piscopo
authors.forename: G
authors.initials: G
authors.lastname: Pulcrano
authors.forename: L
authors.initials: L
authors.lastname: Fucci
mesh_terms: D000818:Animals; D001435:Bacteriophages / Q000235:genetics; D001483:Base Sequence; D003062:Codon; D004261:DNA Replication; D018076:DNA, Complementary / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D015698:Genomic Library; D006657:Histones / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics; D011401:Promoter Regions, Genetic; D012617:Sea Urchins / Q000196:embryology / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D003062:Codon; D018076:DNA, Complementary; D006657:Histones; D009687:Nuclear Proteins