Rabbit EPO gene and cDNA: expression of rabbit EPO after intramuscular injection of pDNA.
Erythropoietin (EPO) cDNA was cloned from kidney total RNA of a NZW rabbit. The cDNA comprises a 588-bp open reading frame encoding a 195 amino acid protein with distinguishable regions of high of homology to other mammalian EPOs. Intramuscular injection of mice with a rabbit EPO expression plasmid resulted in a significant hematocrit increase. A rabbit genomic DNA fragment was also cloned using the rabbit EPO cDNA. This 4312-bp genomic DNA fragment contains sequences homologous to the mouse EPO promoter and hypoxia-responsive enhancer. In addition, the genomic DNA also presents a high degree of conservation to other regions involved in hypoxia response. Sequence divergence in the 3' UTR may indicate differences in regulation of mRNA stability or response to low oxygen tension.
Vilalta
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Department of Molecular Biology, Vical Incorporated, San Diego, California, 92121, USA. avilalta@vical.com
Wu
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Margalith
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Hobart
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P
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D017124:Conserved Sequence; D018076:DNA, Complementary / Q000008:administration & dosage; D004742:Enhancer Elements, Genetic; D004921:Erythropoietin / Q000235:genetics* / Q000378:metabolism; D015316:Genetic Therapy; D006400:Hematocrit; D007273:Injections, Intramuscular; D051379:Mice; D008819:Mice, Nude; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D011817:Rabbits; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D004921:Erythropoietin