Incidence of enteroadherence in diarrhoegenic Escherichia coli in infants in Delhi.
Fifty-six isolates of Escherichia coli including 40 isolates from diarrhoeic infants and 16 from non-diarrhoeic infants were investigated. Twenty-two of the diarrhoeic isolates were typable, the most common serogroup being 086 (33%). None of the non-diarrheic isolates are typable with EPEC antisera with enteropathogenes. Adherence tests with HEp-2 cell line revealed localized adherence in 23%, diffuse adherence in 14% and aggregative adherence in 5.7% of the 35 isolates tested. Aggregative adherence was not observed in any of the EPEC isolates. None of the isolates in the control group exhibited localized or aggregative adherence. However, 25% of these isolates showed diffuse adherence (DA) which was not significantly different from the incidence of DA (34%) in the test group (p > 0.05). The importance of serogrouping and studying adherence pattern of E. coli isolates in establishing their pathogenic potential is thus emphasized.
Varma
M
M
Dept of Microbiology, Lady Hardinge Medical College, New Delhi.
Sachdeva
V
V
Diwan
N
N
D001422:Bacterial Adhesion; D015373:Bacterial Typing Techniques; D003967:Diarrhea / Q000453:epidemiology / Q000382:microbiology*; D004926:Escherichia coli / Q000145:classification / Q000472:pathogenicity / Q000502:physiology*; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000382:microbiology*; D006801:Humans; D007194:India / Q000453:epidemiology; D007223:Infant; D014774:Virulence