Rescue of mouse embryos from 2-cell blocks by microinjection of maturation promoting factor.
OBJECTIVE
To examine the rescue of mouse embryos from 2-cell blocks by the microinjection of maturation promoting factor (MPF) extracted from matured Xenopus eggs into one of the blastomeres of 2-cell stage mouse embryos.


METHODS
Controlled laboratory study.


METHODS
First Department of Obstetrics and Gynecology, Toho University School of Medicine, Tokyo, Japan.


METHODS
Eight- to 10-week-old female Crj:CD-1(ICR) mice.


METHODS
One of the blastomeres of the mouse 2-cell embryos was injected with MPF (MI group) or mHTF medium (MED group) at 28--32 hours after insemination.


METHODS
The developmental rate to blastocyst.


RESULTS
The developmental rate to blastocyst in the MI group (48.0%) was significantly higher than that in the MED group (0%).


CONCLUSIONS
The 2-cell block was specifically rescued by the microinjection of MPF and not by the insertion of pipettes.
Nakano
H
H
First Department of Obstetrics and Gynecology, Toho University School of Medicine, Tokyo, Japan. hide-n@jg7.so-net.ne.jp
Kubo
H
H
D000818:Animals; D002970:Cleavage Stage, Ovum / Q000187:drug effects; D046508:Culture Techniques; D004622:Embryo, Mammalian / Q000166:cytology / Q000187:drug effects; D004625:Embryo, Nonmammalian; D005314:Embryonic and Fetal Development / Q000187:drug effects; D005260:Female; D008297:Male; D016200:Maturation-Promoting Factor / Q000302:isolation & purification / Q000494:pharmacology*; D000090204:Mesothelin; D051379:Mice / Q000196:embryology*; D008813:Mice, Inbred ICR; D008845:Microinjections; D014982:Xenopus laevis / Q000378:metabolism
C000715528:Msln protein, mouse; D016200:Maturation-Promoting Factor; D000090204:Mesothelin