Synthesis and properties of oligonucleotides containing 8-bromo-2'-deoxyguanosine.
The preparation of oligonucleotides containing 8-bromo-2'-deoxyguanosine is described. Substitution of G by 8-bromoguanine on an alternating CG decamer stabilizes the Z-form in such a way that the B-form was not observed. Melting temperatures showed that duplexes in which 8-bromo-2'-deoxyguanosine paired with natural bases were much less stable.
Fàbrega
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European Molecular Biology Laboratory, Heidelberg, Germany.
Macías
M J
MJ
Eritja
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R
D002845:Chromatography / Q000379:methods; D002942:Circular Dichroism; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000032:analysis / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D009943:Organophosphorus Compounds / Q000737:chemistry
C439365:8-bromo-2'-deoxyguanosine; D009841:Oligonucleotides; D009943:Organophosphorus Compounds; C434331:phosphoramidite; D003849:Deoxyguanosine