Development of NMR instrumentation to achieve excitation of large bandwidths in high-resolution spectra at high field.
A prototype 2.5-mm (1)H high-resolution probe for an 18.8-T (800 MHz) nuclear magnetic resonance spectrometer has been designed, together with a dedicated amplifier capable of delivering up to 1 kW of power. This probe permits a 90 degrees pulse length of 2 mus to be achieved at 300 W, corresponding to an excitation bandwidth of +/-125 kHz. Probe performances were tested on samples commonly used for this purpose as well as on protein and paramagnetic model compound samples. It is shown that this probe is useful for a wide range of applications at high magnetic field, especially in the study of systems characterized by very broad and far-shifted resonances and in experiments that require high-power radiofrequency irradiation.
Luchinat
C
C
Magnetic Resonance Center, University of Florence, I-50019 Sesto Fiorentino, Florence, Italy.
Piccioli
M
M
Pierattelli
R
R
Engelke
F
F
Marquardsen
T
T
Ruin
R
R