A beta-lactamase belonging to group 2e from oral clinical isolates of Prevotella intermedia.
A beta-lactamase in oral clinical isolates of Prevotella intermedia that hydrolyzed cefuroxime and cephalothin with rates of 600 and 53.3 respectively, relative to that for cephaloridine (100), was characterized as a 2e-cephalosporinase. Inhibition was observed by clavulanic acid (IC50 0.72 microM), tazobactam (IC50 0.21 microM) and sulbactam (IC50 0.07 microM) and was not inhibited by cloxacillin, EDTA, NaCl or p-CMB. The pI and pH optima were 4.7 and 5.4, respectively.
Valle
G
G
Laboratory of Oral Microbiology, Faculty of Medicine, University of Oviedo, 3306 Oviedo, Spain.
Quirós
L M
LM
Andrés
M T
MT
Fierro
J F
JF
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D016866:Bacteroidaceae Infections / Q000382:microbiology*; D002511:Cephalosporins / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D010518:Periodontitis / Q000382:microbiology; D018713:Prevotella intermedia / Q000201:enzymology*; D065093:beta-Lactamase Inhibitors; D001618:beta-Lactamases / Q000302:isolation & purification* / Q000378:metabolism
D000900:Anti-Bacterial Agents; D002511:Cephalosporins; D065093:beta-Lactamase Inhibitors; D001618:beta-Lactamases