title: N- and C-terminal halves of human annexin VI differ in ability to form low pH-induced ion channels.
abstract: Human recombinant annexin VI (AnxVI) or its N- (AnxVIA) and C-terminal (AnxVIB) fragments were expressed in E. coli. Their ability to form voltage-dependent ion channels in membranes, induced by low pH, was measured to verify the hypothesis that, upon acidification, the hydrophobicity of AnxVI at a specific domain significantly increases allowing the AnxVI interaction with lipids in a Ca(2+)-independent manner. By theoretically analyzing changes in protein hydrophobicity, we found that hydrophobicity of AnxVIA significantly differed from that of AnxVIB at low pH. These predictions were confirmed experimentally by using planar lipid bilayers and liposome pull-down assay. We found striking difference between AnxVIA and AnxVIB in the ion channel activity, as well as in the membrane binding, suggesting that the halves of AnxVI maybe functionally different. Moreover, we calculated and predicted that the ion channel activity at low pH should appear in other human annexins, as AnxII, AnxV (as known), AnxVIII, and AnxXIII. The possibility that AnxVI acts as cytosolic component of a transmembrane pH-sensing mechanism is proposed.
authors.affiliation: Department of Cellular Biochemistry, Nencki Institute of Experimental Biology, 3 Pasteur Street, 02-093 Warsaw, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Golczak
authors.forename: A
authors.initials: A
authors.lastname: Kirilenko
authors.forename: J
authors.initials: J
authors.lastname: Bandorowicz-Pikula
authors.forename: S
authors.initials: S
authors.lastname: Pikula
mesh_terms: D017317:Annexin A6 / Q000737:chemistry* / Q000502:physiology; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007473:Ion Channels / Q000737:chemistry / Q000502:physiology; D008051:Lipid Bilayers / Q000378:metabolism; D010446:Peptide Fragments / Q000737:chemistry / Q000502:physiology; D011994:Recombinant Proteins / Q000378:metabolism; D014162:Transfection
chemical_list: D017317:Annexin A6; D007473:Ion Channels; D008051:Lipid Bilayers; D010446:Peptide Fragments; D011994:Recombinant Proteins