title: Cloning and characterization of a chitosanase gene from the koji mold Aspergillus oryzae strain IAM 2660.
abstract: A genomic copy of the gene coding for chitosanase (csnA) was isolated from Aspergillus oryzae IAM 2660. A. oryzae csnA contains an open reading frame that encodes a polypeptide of 245 amino acids with a calculated molecular mass of 26,500 Da. The deduced amino acid sequence of A. oryzae csnA indicates extensive similarities to those of other fungal chitosanases.
authors.affiliation: Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, Ueda, Nagano, Japan.
authors.forename: X Y
authors.initials: XY
authors.lastname: Zhang
authors.forename: A L
authors.initials: AL
authors.lastname: Dai
authors.forename: K
authors.initials: K
authors.lastname: Kuroiwa
authors.forename: R
authors.initials: R
authors.lastname: Kodaira
authors.forename: M
authors.initials: M
authors.lastname: Nogawa
authors.forename: M
authors.initials: M
authors.lastname: Shimosaka
authors.forename: M
authors.initials: M
authors.lastname: Okazaki
mesh_terms: D000595:Amino Acid Sequence; D001232:Aspergillus fumigatus / Q000201:enzymology / Q000235:genetics; D001236:Aspergillus oryzae / Q000201:enzymology* / Q000235:genetics*; D003001:Cloning, Molecular; D005670:Fusarium / Q000201:enzymology / Q000235:genetics; D015723:Gene Library; D006026:Glycoside Hydrolases / Q000235:genetics*; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013045:Species Specificity
chemical_list: D006026:Glycoside Hydrolases; C019868:chitosanase