Antigen presentation using novel particulate organelles from halophilic archaea.
A presentation vehicle was developed based on particulate gas vesicles produced by halophilic archaea. Gas vesicle epitope displays were prepared using standard coupling methods or recombinant DNA technology. When presented in the context of gas vesicle preparations, either the hapten, TNP, or a model six amino acid recombinant insert in the outer gas vesicle protein, GvpC was rendered immunogenic. Assays to quantify humoral responses indicated that each preparation elicited strong antibody responses in the absence of exogenous adjuvant. Thus, each preparation elicited a humoral response when injected into mice and this response was long lived and exhibited immunologic memory. Recombinant gas vesicle preparations therefore constitute a new, self-adjuvanting carrier/display vehicle for presentation of an array of peptidyl epitopes.
Stuart
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Department of Microbiology, University of Massachusetts, Amherst, MA 01003, USA. esstuart@microbio.umass.edu
Morshed
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Sremac
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DasSarma
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D000276:Adjuvants, Immunologic; D000818:Animals; D000941:Antigens / Q000737:chemistry* / Q000276:immunology; D019843:Archaeal Proteins; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D006217:Halobacterium / Q000166:cytology* / Q000235:genetics; D006241:Haptens / Q000737:chemistry / Q000276:immunology; D007106:Immune Sera; D007156:Immunologic Memory; D008565:Membrane Proteins; D051379:Mice; D008807:Mice, Inbred BALB C; D015388:Organelles / Q000737:chemistry / Q000276:immunology*; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology; D010851:Picrates / Q000737:chemistry / Q000276:immunology; D011506:Proteins; D012710:Serum Albumin, Bovine / Q000737:chemistry
D000276:Adjuvants, Immunologic; D000941:Antigens; D019843:Archaeal Proteins; D001425:Bacterial Outer Membrane Proteins; D006241:Haptens; D007106:Immune Sera; D008565:Membrane Proteins; D010455:Peptides; D010851:Picrates; D011506:Proteins; C038524:gas vesicle protein; D012710:Serum Albumin, Bovine; C005858:picric acid