title: The onset of collagen synthesis in sea urchin embryos.
abstract: In Arbacia punctulata and Strongylocentrotus purpuratus, two species of sea urchins, collagen synthesis begins during gastrulation and increases many-fold before reaching a plateau in the late pluteus stage. A collagen extraction method involving treatment with 0.1 M NaOH and hot 10% trichloroacetic acid provided the basis for a sensitive assay of collagen synthesis.
authors.affiliation: Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, Mass. 02138, USA.
authors.forename: R
authors.initials: R
authors.lastname: Golob
authors.forename: C J
authors.initials: CJ
authors.lastname: Chetsanga
authors.forename: P
authors.initials: P
authors.lastname: Doty
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000378:metabolism; D003094:Collagen / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism*; D004625:Embryo, Nonmammalian / Q000378:metabolism*; D005775:Gastrula / Q000378:metabolism; D006909:Hydroxyproline / Q000032:analysis; D009924:Organ Culture Techniques; D012617:Sea Urchins / Q000196:embryology / Q000378:metabolism*; D013997:Time Factors; D014316:Tritium
chemical_list: D014316:Tritium; D003094:Collagen; D006909:Hydroxyproline