A transgenic model for listeriosis: role of internalin in crossing the intestinal barrier.
Listeria monocytogenes is responsible for severe food-borne infections, but the mechanisms by which bacteria cross the intestinal barrier are unknown. Listeria monocytogenes expresses a surface protein, internalin, that interacts with a host receptor, E-cadherin, to promote entry into human epithelial cells. Murine E-cadherin, in contrast to guinea pig E-cadherin, does not interact with internalin, excluding the mouse as a model for addressing internalin function in vivo. In guinea pigs and transgenic mice expressing human E-cadherin, internalin was found to mediate invasion of enterocytes and crossing of the intestinal barrier. These results illustrate how relevant animal models for human infections can be generated.
Lecuit
M
M
Unité des Interactions Bactéries-Cellules, Station Centrale de Microscopie Electronique, Institut Pasteur, 75724 Paris cedex 15, France.
Vandormael-Pournin
S
S
Lefort
J
J
Huerre
M
M
Gounon
P
P
Dupuy
C
C
Babinet
C
C
Cossart
P
P
D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism*; D018988:Bacterial Translocation; D015820:Cadherins / Q000235:genetics / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics; D015169:Colony Count, Microbial; D004195:Disease Models, Animal; D020895:Enterocytes / Q000378:metabolism / Q000382:microbiology*; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D006168:Guinea Pigs; D006801:Humans; D007413:Intestinal Mucosa / Q000382:microbiology / Q000473:pathology; D007421:Intestine, Small / Q000382:microbiology / Q000473:pathology; D008089:Listeria monocytogenes / Q000254:growth & development / Q000378:metabolism / Q000472:pathogenicity*; D008088:Listeriosis / Q000382:microbiology* / Q000473:pathology; D008099:Liver / Q000382:microbiology / Q000473:pathology; D008198:Lymph Nodes / Q000382:microbiology / Q000473:pathology; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D011401:Promoter Regions, Genetic; D013154:Spleen / Q000382:microbiology / Q000473:pathology; D019076:Transgenes; D025521:Tumor Suppressor Proteins; D014774:Virulence
D001426:Bacterial Proteins; D015820:Cadherins; D002352:Carrier Proteins; C107608:FABP7 protein, human; C082667:Fabp5 protein, mouse; C086936:Fabp7 protein, mouse; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D025521:Tumor Suppressor Proteins; C069761:internalin protein, Bacteria