title: The 5' untranslated region of the At-P5R gene is involved in both transcriptional and post-transcriptional regulation.
abstract: The steady-state level of transcripts coding for the pyrroline-5-carboxylate reductase of Arabidopsis (At-P5R) increased under salt and heat stress, mainly because of an enhanced mRNA stability. However, the At-P5R protein level was not induced, and its translation was inhibited at initiation stage and probably also at later stages. Replacement of the 5' untranslated region (5'UTR) and beta-glucuronidase (gus) fusion analysis revealed that the first 92 bp region of the At-P5R 5'UTR was sufficient to mediate transcript stabilization and translation inhibition during salt and heat stresses. Furthermore, the first 92 bp region of the At-P5R 5'UTR was also involved in transcription efficiency in a promoter-dependent manner. The results demonstrated that the stress regulation of At-P5R is complex and involves the 5'UTR which acts at three levels, partly in opposing directions.
authors.affiliation: Vakgroep Moleculaire Genetica & Departement Plantengenetica, Vlaams Interuniversitair Instituut voor Biotechnologie, Universiteit Gent, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium.
authors.forename: X J
authors.initials: XJ
authors.lastname: Hua
authors.forename: B
authors.initials: B
authors.lastname: Van de Cotte
authors.forename: M
authors.initials: M
authors.lastname: Van Montagu
authors.forename: N
authors.initials: N
authors.lastname: Verbruggen
mesh_terms: D020121:5' Untranslated Regions; D017360:Arabidopsis / Q000235:genetics*; D001483:Base Sequence; D015152:Blotting, Northern; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D005966:Glucuronidase / Q000235:genetics; D006358:Hot Temperature; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics*; D011132:Polyribosomes / Q000235:genetics; D011401:Promoter Regions, Genetic; D014176:Protein Biosynthesis; D011762:Pyrroline Carboxylate Reductases / Q000235:genetics*; D012313:RNA; D012323:RNA Processing, Post-Transcriptional; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018749:RNA, Plant; D011993:Recombinant Fusion Proteins; D012965:Sodium Chloride; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic; D000097502:delta-1-Pyrroline-5-Carboxylate Reductase
chemical_list: D020121:5' Untranslated Regions; D005966:Glucuronidase; D010940:Plant Proteins; D011762:Pyrroline Carboxylate Reductases; D012313:RNA; D012333:RNA, Messenger; D018749:RNA, Plant; D011993:Recombinant Fusion Proteins; D012965:Sodium Chloride; D014157:Transcription Factors; D000097502:delta-1-Pyrroline-5-Carboxylate Reductase; C034477:RNA primers