Formation of a secondary corpus luteum after ultrasound-guided follicular aspiration in cows.
This paper reports the observed formation of a secondary corpus luteum (CL) in the presence of the cyclic corpus luteum, on the ovaries of a cow after ultrasound-guided follicular aspiration for oocyte recovery. The secondary structure, although smaller and lighter (4.97 g vs. 6.02 g) than the natural one, had the typical macroscopic appearance of a corpus luteum. Histological examination of the structure using electron microscopy revealed typical structural features of a natural CL. Mean tissue progesterone concentration was significantly lower in the secondary CL (31.15 +/- 3.11 compared with 58.29 +/- 6.32 micrograms/g tissue of the cyclic CL) and oestradiol-17 beta significantly higher than in the natural CL (108 +/- 11.6 compared with 74.2 +/- 7.81 pg/g tissue). P450scc and P450(17 alpha) mRNA was detected in both structures while P450arom and full-length mRNA FSH receptor were detected only in the secondary structure.
Amiridis
G S
GS
Department of Reproduction and Obstetrics, Faculty of Veterinary Medicine, University of Thessaly, Terma Trikalon, 43100 Karditsa, Greece. amiridis@spark.net.gr
Robertson
L
L
Jeffcoate
I A
IA
Belibasaki
S
S
Boyd
J S
JS
O'Shaughnessy
P J
PJ
D000818:Animals; D002417:Cattle / Q000502:physiology*; D003338:Corpus Luteum / Q000002:abnormalities* / Q000648:ultrastructure; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis; D005260:Female; D005307:Fertilization in Vitro / Q000662:veterinary; D001239:Inhalation; D008854:Microscopy, Electron; D009865:Oocytes; D006080:Ovarian Follicle; D012333:RNA, Messenger / Q000032:analysis; D018084:Ultrasonography, Interventional / Q000009:adverse effects / Q000662:veterinary*
D012333:RNA, Messenger; D003577:Cytochrome P-450 Enzyme System