Mouse hepatocyte growth factor activator inhibitor type 1 (HAI-1) and type 2 (HAI-2)/placental bikunin genes and their promoters.
Hepatocyte growth factor (HGF) activator inhibitor type 1 (HAI-1) and type 2 (HAI-2) were recently discovered as specific inhibitors of HGF activator. Each of them contains two Kunitz-type serine protease inhibitor domains and a transmembrane domain, so that their overall structures are similar to each other. In this study, mouse genes encoding HAI-1 and HAI-2 were cloned by screening of a mouse genomic bacterial artificial chromosome library and by polymerase chain reaction of mouse genomic DNA, respectively. The genes (mHAI-1 and mHAI-2) were defined to consist of 11 and eight exons spanning 11 kbp and 9.5 kbp, respectively. Neither a TATA nor CAAT box was found in 5'-flanking regions of both genes and no apparent homologous portion was observed between mHAI-1 and mHAI-2 promoter regions. Promoter assay of mHAI-1 and human HAI-1 revealed that the potential binding sites of a complex of Egr-1-3 and Sp1, which was well-conserved between human (-42 to -58) and mouse (-44 to -57), might be a key portion of its transcriptional regulation to function as not only house-keeping but also early responsive genes.
Itoh
H
H
Second Department of Pathology, Miyazaki Medical College, Kiyotake, Japan. hitoh@post.miyazaki-med.ac.jp
Kataoka
H
H
Meng
J Y
JY
Hamasuna
R
R
Kitamura
N
N
Koono
M
M
D000818:Animals; D001483:Base Sequence; D006367:HeLa Cells; D006801:Humans; D008562:Membrane Glycoproteins / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D010920:Placenta / Q000378:metabolism; D011401:Promoter Regions, Genetic; D053491:Proteinase Inhibitory Proteins, Secretory; D012697:Serine Endopeptidases; D014360:Trypsin Inhibitor, Kunitz Soybean
D008562:Membrane Glycoproteins; D053491:Proteinase Inhibitory Proteins, Secretory; C109149:SPINT1 protein, human; C109148:SPINT2 protein, human; C504591:Spint1 protein, mouse; D014360:Trypsin Inhibitor, Kunitz Soybean; C081092:HGF activator; D012697:Serine Endopeptidases