Normal IncA expression and fusogenicity of inclusions in Chlamydia trachomatis isolates with the incA I47T mutation.
To investigate the correlation between the incA I47T mutation in Chlamydia trachomatis and the nonfusogenic phenotype, the incA genes of 25 isolates were sequenced. Four major sequence types were identified. Seven isolates (28%) had the I47T mutation. Isolates representing the four sequence types expressed IncA in the membrane of one large single inclusion. In conclusion, the incA I47T mutation is not associated with the nonfusogenic phenotype.
Pannekoek
Y
Y
Department of Medical Microbiology, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands. y.pannekoek@amc.uva.nl
van der Ende
A
A
Eijk
P P
PP
van Marle
J
J
de Witte
M A
MA
Ossewaarde
J M
JM
van den Brule
A J
AJ
Morré
S A
SA
Dankert
J
J
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D002692:Chlamydia trachomatis / Q000235:genetics* / Q000302:isolation & purification; D004269:DNA, Bacterial; D015870:Gene Expression; D002479:Inclusion Bodies; D008969:Molecular Sequence Data; D009154:Mutation; D010750:Phosphoproteins / Q000235:genetics*
D001426:Bacterial Proteins; D004269:DNA, Bacterial; C094163:IncA protein, bacteria; D010750:Phosphoproteins