Determination of bisphenol A in blood using high-performance liquid chromatography-electrochemical detection with solid-phase extraction.
A method for the determination of bisphenol A (BPA) in blood was investigated using high-performance liquid chromatography-electrochemical detection (HPLC-ED) with solid-phase extraction. When BPA at the concentrations of 25-100 ng/ml were added to whole blood, BPA recoveries were 26-48%. When BPA was added to water, plasma or hemolyzed red blood cells (H-RBC), BPA recoveries in water and plasma were almost similar (94%). However, the recovery in H-RBC was very low (36-46%). When BPA and plasma were added to H-RBC, the recovery was 70-85%. In authentic bovine metHb solution, BPA decreased depending on the metHb concentration, however, BPA recovery in the solution added with more than 17% plasma was higher than that in metHb only. These suggest that metHb influences the BPA recovery in whole blood. However, an accurate determination of BPA using HPLC was easily made possible by separating RBC from plasma.
Sajiki
J
J
Public Health Laboratory of Chiba Prefecture, Japan. sajiki@beige.ocn.ne.jp
D000818:Animals; D001559:Benzhydryl Compounds; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004563:Electrochemistry; D004912:Erythrocytes / Q000737:chemistry / Q000187:drug effects; D004968:Estrogens, Non-Steroidal / Q000032:analysis* / Q000097:blood; D006461:Hemolysis; D006801:Humans; D008706:Methemoglobin / Q000032:analysis / Q000494:pharmacology; D010636:Phenols / Q000032:analysis* / Q000097:blood; D015203:Reproducibility of Results; D012756:Sheep; D012977:Sodium Nitrite / Q000494:pharmacology; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D004968:Estrogens, Non-Steroidal; D008706:Methemoglobin; D010636:Phenols; D012977:Sodium Nitrite; C006780:bisphenol A; D000098826:Bisphenol A Compounds