Acoustic and optical transduction of BuChE binding to procainamide modified surfaces.
A novel polymer, poly(procainamide), PPA, containing numerous binding sites for cholinesterases was synthesized as a recognition layer for butyryl cholinesterase (BuChE) interaction with the ligand procainamide, utilizing TSM and SPR sensors. The polymer was synthesized by the reaction of methacryloyl chloride and procainamide followed by radical polymerization. Sensor surfaces (Au or SiO(2)) were spin-coated by the polymer solution to form thin layers. Binding of BuChE was found to be sensitive to the drying procedure of the polymer layer. The binding of BuChE to the polymer coated sensors was monitored on-line by following the response of thickness shear mode (TSM) and surface plasmon resonance (SPR) sensors. Binding of BuChE to PPA-coated TSM sensors were shown to follow a Langmuir isotherm giving association constant 3.4x10(6) M(-1).
Marx
S
S
Department of Physical Chemistry, Israel Institute for Biological Research, P.O. Box 19, Ness-Ziona 70450, Israel.
Kaushansky
N
N
Gratziany
N
N
Barness
I
I
Liron
Z
Z
D015374:Biosensing Techniques; D044926:Butyryl-CoA Dehydrogenase; D044943:Fatty Acid Desaturases / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D011342:Procainamide / Q000737:chemistry*; D011485:Protein Binding; D013499:Surface Properties
D011108:Polymers; D044943:Fatty Acid Desaturases; D044926:Butyryl-CoA Dehydrogenase; D011342:Procainamide