Mouse model of Bell's palsy induced by reactivation of herpes simplex virus type 1.
In order to investigate the mechanism of Bell's palsy, we developed an animal model of facial nerve paralysis induced by the reactivation of herpes simplex virus type 1 (HSV-1). Eight weeks after recovery from facial nerve paralysis caused by inoculation with HSV-1, the mice were treated with auricular skin scratch at the site of the previous inoculation, or with intraperitoneal injection of anti-CD3 monoclonal antibody (mAb), or combination of both procedures. No mice developed facial nerve paralysis when they were treated with either auricular scratch or mAb injection alone. In contrast, 20% of mice developed facial nerve paralysis with the combined treatment. With one exception, no mouse treated with either auricular scratch or mAb injection showed HSV-I DNA in their facial nerve tissue, whereas 4 out of 6 mice receiving both treatments showed HSV-1 DNA on day 10 after treatment. Histopathological findings showed neuronal degeneration in the geniculate ganglion and demyelination of the facial motor nerve in paralyzed mice. These findings suggest that a combination of stimuli, local skin irritation, and general immunosuppression is essential for successfully inducing facial nerve paralysis in mice with latent HSV-1 infection.
Takahashi
H
H
Department of Otolaryngology, Ehime University School of Medicine, Onsen-gun, Japan.
Hitsumoto
Y
Y
Honda
N
N
Hato
N
N
Mizobuchi
M
M
Murakami
S
S
Kisaki
H
H
Wakisaka
H
H
Gyo
K
K
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D020330:Bell Palsy / Q000821:virology*; D004279:DNA, Viral / Q000032:analysis; D004195:Disease Models, Animal; D004431:Ear, External / Q000293:injuries; D005260:Female; D005830:Geniculate Ganglion / Q000473:pathology / Q000821:virology; D006561:Herpes Simplex / Q000097:blood / Q000235:genetics / Q000473:pathology / Q000821:virology*; D018655:Lymphocyte Count; D051379:Mice; D008807:Mice, Inbred BALB C; D018139:Simplexvirus / Q000145:classification / Q000502:physiology*; D013601:T-Lymphocytes / Q000473:pathology; D014775:Virus Activation; D017735:Virus Latency
D000911:Antibodies, Monoclonal; D004279:DNA, Viral