title: The RNA 5 of Prunus necrotic ringspot virus is a biologically inactive copy of the 3'-UTR of the genomic RNA 3.
abstract: In addition to the four RNAs known to be encapsidated by Prunus necrotic ringspot virus (PNRSV) and Apple mosaic virus (ApMV), an additional small RNA (RNA 5) was present in purified preparations of several isolates of both viruses. RNA 5 was always produced following infection of a susceptible host by an artificial mixture of RNAs 1, 2, 3, and 4 indicating that it was a product of viral replication. RNA 5 does not activate the infectivity of mixtures that contain the three genomic RNAs (RNA 1 + RNA 2 + RNA 3) nor does it appear to modify symptom expression. Results from hybridization studies suggested that RNA 5 had partial sequence homology with RNAs 1, 2, 3, and 4. Cloning and sequencing the RNA 5 of isolate CH 57/1-M of PNRSV, and the 3' termini of the RNA 1, RNA 2 and RNA 3 of this isolate indicated that it was a copy of the 3' untranslated terminal region (3'-UTR) of the genomic RNA 3.
authors.affiliation: Istituto Agronomico Mediterraneo, Valenzano, Italy.
authors.forename: B
authors.initials: B
authors.lastname: Di Terlizzi
authors.forename: L J
authors.initials: LJ
authors.lastname: Skrzeczkowski
authors.forename: G I
authors.initials: GI
authors.lastname: Mink
authors.forename: S W
authors.initials: SW
authors.lastname: Scott
authors.forename: M T
authors.initials: MT
authors.lastname: Zimmerman
mesh_terms: D020413:3' Untranslated Regions; D001483:Base Sequence; D002213:Capsid / Q000235:genetics*; D036022:Capsid Proteins; D019179:Ilarvirus / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000821:virology*; D012367:RNA, Viral / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid
chemical_list: D020413:3' Untranslated Regions; D036022:Capsid Proteins; D012367:RNA, Viral; C087151:RNA 3 coat protein, prune dwarf ilarvirus