title: Effect of oral administration of high vitamin C and E dosages on the gilthead seabream (Sparus aurata L.) innate immune system.
abstract: The effect of the oral administration of high dosages of vitamins C and E on the innate immune system of the seabream was investigated. Gilthead seabream (Sparus aurata L.) were fed four different diets for 45 days: a commercial diet as control, a 3g/kg vitamin C-supplemented diet, a 1.2g/kg vitamin E-supplemented diet or a diet containing both vitamin supplements. After 15, 30 and 45 days, serum ascorbic acid and alpha-tocopherol levels, growth, complement titers and head-kidney leucocyte phagocytic and respiratory burst activities were evaluated. The results showed that serum vitamin levels reflected dietary input. Fish fed the vitamin C-supplemented diet showed an enhanced respiratory burst activity, while fish fed the vitamin E-supplemented diet exhibited increased complement and phagocytic activities. All of these positive effects were found in fish fed the joint diet, as well as a synergistically enhanced respiratory burst activity at day 30. The results demonstrate that in vivo vitamins C and E exert a synergistic effect enhancing the respiratory burst activity of seabream phagocytes.
authors.affiliation: Department of Cell Biology, Faculty of Biology, University of Murcia, 30071, Murcia, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Ortuño
authors.forename: A
authors.initials: A
authors.lastname: Cuesta
authors.forename: M
authors.initials: M
authors.lastname: Angeles Esteban
authors.forename: J
authors.initials: J
authors.lastname: Meseguer
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001205:Ascorbic Acid / Q000097:blood / Q000494:pharmacology*; D001835:Body Weight / Q000187:drug effects; D015941:Complement Hemolytic Activity Assay; D003165:Complement System Proteins / Q000187:drug effects / Q000276:immunology; D005434:Flow Cytometry / Q000662:veterinary; D007962:Leukocytes / Q000187:drug effects / Q000276:immunology; D010587:Phagocytosis / Q000187:drug effects; D011897:Random Allocation; D016897:Respiratory Burst / Q000187:drug effects; D021541:Sea Bream / Q000276:immunology*; D014810:Vitamin E / Q000097:blood / Q000494:pharmacology*
chemical_list: D014810:Vitamin E; D003165:Complement System Proteins; D001205:Ascorbic Acid