Constitutive expression and delayed light response of casein kinase Iepsilon and Idelta mRNAs in the mouse suprachiasmatic nucleus.
Casein kinase Iepsilon (CKIepsilon) and casein kinase Idelta (CKIdelta) phosphorylate clock oscillating mPER proteins, and play a key role in the transcription (post)translation feedback loop that generates circadian rhythm. In the present study, the expression profiles of CKIepsilon and CKIdelta mRNAs were examined in the mice clock center, suprachiasmatic nucleus (SCN). Moderate levels of CKIepsilon and CKIdelta mRNAs were constantly expressed in the SCN in both light:dark and constant dark conditions. This finding supports the hypothesis that CKI may form a constant threshold to the nuclear entry of mPER proteins as in the Drosophila homologue, double-time. Further, we demonstrated that the light exposure at subjective night induced a delayed increase in CKIepsilon and CKIdelta mRNAs in the SCN. CKIepsilon and CKIdelta proteins may play a role on light-induced phase-shift.
Ishida
Y
Y
Department of Brain Sciences, Kobe University Graduate School of Medicine, Kobe, Japan.
Yagita
K
K
Fukuyama
T
T
Nishimura
M
M
Nagano
M
M
Shigeyoshi
Y
Y
Yamaguchi
S
S
Komori
T
T
Okamura
H
H
D000818:Animals; D047388:Casein Kinases; D018797:Cell Cycle Proteins; D002940:Circadian Rhythm / Q000502:physiology*; D003624:Darkness; D015971:Gene Expression Regulation, Enzymologic; D008029:Lighting; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009687:Nuclear Proteins / Q000235:genetics; D056950:Period Circadian Proteins; D011494:Protein Kinases / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D013493:Suprachiasmatic Nucleus / Q000201:enzymology*
D018797:Cell Cycle Proteins; D009687:Nuclear Proteins; C539023:Per1 protein, mouse; D056950:Period Circadian Proteins; D012333:RNA, Messenger; D011494:Protein Kinases; D047388:Casein Kinases