Optical visualization of microsomal degranulation by a carcinogen.
We report here for the first time the optical visualization of microsomal degranulation induced by a carcinogen (p-dimethylaminoazobenzene), employing analytical ultracentrifugation. Our observations demonstrate that electrophiles of a carcinogen can disrupt ribosome-membrane interaction in rough microsomes by their attacks on nucleophilic components of the reticular membrane-ribosome complex involved in protein synthesis for export from the cytosol. Lack of exported proteins can adversely affect signal transduction across plasma membrane, possibly leading to carcinogenesis.
Dani
H M
HM
Biochemistry Department, University College, London. harryd8@yahoo.com
Kaur
P
P
D000818:Animals; D002273:Carcinogens / Q000494:pharmacology*; D002458:Cell Fractionation; D018870:Endoplasmic Reticulum, Rough / Q000187:drug effects; D066298:In Vitro Techniques; D008861:Microsomes / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014461:Ultracentrifugation / Q000379:methods; D004124:p-Dimethylaminoazobenzene / Q000494:pharmacology*
D002273:Carcinogens; D004124:p-Dimethylaminoazobenzene