title: Cactus-independent nuclear translocation of Drosophila RELISH.
abstract: Insects can effectively and rapidly clear microbial infections by a variety of innate immune responses including the production of antimicrobial peptides. Induction of these antimicrobial peptides in Drosophila has been well established to involve NF-kappaB elements. We present evidence here for a molecular mechanism of Lipopolysaccharide (LPS)-induced signaling involving Drosophila NF-kappaB, RELISH, in Drosophila S2 cells. We demonstrate that LPS induces a rapid processing event within the RELISH protein releasing the C-terminal ankyrin-repeats from the N-terminal Rel homology domain (RHD). Examination of the cellular localization of RELISH reveals that the timing of this processing coincides with the nuclear translocation of the RHD and the retention of the ankyrin-repeats within the cytoplasm. Both the processing and the nuclear translocation immediately precede the expression of antibacterial peptide genes cecropin A1, attacin, and diptericin. Over-expression of the RHD but not full-length RELISH results in an increase in the promoter activity of the cecropin A1 gene in the absence of LPS. Furthermore, the LPS-induced expression of these antibacterial peptides is greatly reduced when RELISH expression is depleted via RNA-mediated interference. In addition, loss of cactus expression via RNAi revealed that RELISH activation and nuclear translocation is not dependent on the presence of cactus. Taken together, these results suggest that this signaling mechanism involving the processing of RELISH followed by nuclear translocation of the RHD is central to the induction of at least part of the antimicrobial response in Drosophila, and is largely independent of cactus regulation.
authors.affiliation: Department of Gene Expression Sciences, GlaxoSmithKline Pharmaceuticals, King of Prussia, Pennsylvania, USA.
authors.forename: W D
authors.initials: WD
authors.lastname: Cornwell
authors.forename: R B
authors.initials: RB
authors.lastname: Kirkpatrick
mesh_terms: D021581:Active Transport, Cell Nucleus / Q000187:drug effects / Q000502:physiology; D000818:Animals; D017089:Ankyrin Repeat / Q000187:drug effects / Q000502:physiology; D000890:Anti-Infective Agents / Q000378:metabolism; D023181:Antimicrobial Cationic Peptides / Q000096:biosynthesis* / Q000378:metabolism; D002478:Cells, Cultured / Q000166:cytology / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D004330:Drosophila / Q000166:cytology; D029721:Drosophila Proteins; D019476:Insect Proteins / Q000096:biosynthesis / Q000378:metabolism; D008070:Lipopolysaccharides / Q000008:administration & dosage* / Q000378:metabolism; D016328:NF-kappa B / Q000187:drug effects* / Q000378:metabolism; D010750:Phosphoproteins / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology; D014157:Transcription Factors / Q000187:drug effects* / Q000378:metabolism
chemical_list: D000890:Anti-Infective Agents; D023181:Antimicrobial Cationic Peptides; C488990:AttA protein, Drosophila; C488991:AttB protein, Drosophila; C488989:AttC protein, Drosophila; D004268:DNA-Binding Proteins; C054756:DptA protein, Drosophila; D029721:Drosophila Proteins; D019476:Insect Proteins; D008070:Lipopolysaccharides; D016328:NF-kappa B; D010750:Phosphoproteins; C101626:Rel protein, Drosophila; D014157:Transcription Factors; C039711:attacin antibacterial protein, insect; C077938:cact protein, Drosophila; C031161:cecropin A