Topical and oral administration of the natural water-soluble antioxidant from spinach reduces the multiplicity of papillomas in the Tg.AC mouse model.
The Tg.AC mouse carrying the v-Ha-ras structural gene is a useful model for the study of chemical carcinogens, especially those acting via non-genotoxic mechanisms. This study evaluated the efficacy of the non-toxic, water-soluble antioxidant from spinach, natural antioxidant (NAO), in reducing skin papilloma induction in female hemizygous Tg.AC mice treated dermally five times over 2.5 weeks with 2.5 microg 12-O-tetradecanoylphorbol-13-acetate (TPA). The TPA-only group was considered as a control; the other two groups received, additionally, NAO topically (2 mg) or orally (100 mg/kg), 5 days/week for 5 weeks. Papilloma counts made macroscopically during the clinical observations showed a significant decrease in multiplicity (P<0.01) in the NAO topically treated group. According to histological criteria, papilloma multiplicity were lower in both topical-NAO and oral-NAO groups, but significantly so only in the oral-NAO mice (P<0.01). The beneficial effect of NAO in the Tg.AC mouse is reported.
Nyska
A
A
Laboratory of Experimental Pathology, National Institute of Environmental Health Sciences (NIEHS), Research Triangle Park, NC 27709, USA. nyska@niehs.nih.gov
Lomnitski
L
L
Spalding
J
J
Dunson
D B
DB
Goldsworthy
T L
TL
Ben-Shaul
V
V
Grossman
S
S
Bergman
M
M
Boorman
G
G
D000279:Administration, Cutaneous; D000284:Administration, Oral; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001835:Body Weight / Q000187:drug effects; D002273:Carcinogens / Q000009:adverse effects; D004195:Disease Models, Animal; D005260:Female; D011905:Genes, ras / Q000235:genetics; D005838:Genotype; D051379:Mice; D008822:Mice, Transgenic; D010212:Papilloma / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D010936:Plant Extracts / Q000494:pharmacology; D012878:Skin Neoplasms / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D018724:Spinacia oleracea / Q000737:chemistry; D016019:Survival Analysis; D013755:Tetradecanoylphorbol Acetate / Q000009:adverse effects
D000975:Antioxidants; D002273:Carcinogens; D010936:Plant Extracts; D013755:Tetradecanoylphorbol Acetate