Simple high-performance liquid chromatographic assay for the determination of ciprofloxacin in human plasma with ultraviolet detection.
A simple high-performance liquid chromatographic method is described for the quantitative analysis of ciprofloxacin in human plasma. Following protein precipitation from plasma by means of 6% perchloric acid, the upper layer which contains the analyte and the internal standard lomefloxacin, was analysed on a reverse phase column LiChrospher 60 RP-select B (5 microm) (EcoCART 125-3) with ultraviolet detection at 280 nm. The mobile phase was acetic acid 5%-methanol-acetonitrile (90:5:5, v/v/v). The assay was linear for ciprofloxacin over the concentration range of 0.050 to 6.00 microg ml(-1). The limit of quantification (LOQ) was 0.050 microg ml(-1). The method was successfully applied to a bioavailability study with five different ciprofloxacin formulations.
Maya
M T
MT
Centro de Metabolismos e Genética, Faculdade de Farmáicia da Universidade de Lisboa, Portugal. mmaya@correio.ff.ul.pt
Gonçalves
N J
NJ
Silva
N B
NB
Morais
J A
JA
D000890:Anti-Infective Agents / Q000097:blood* / Q000737:chemistry / Q000592:standards; D001682:Biological Availability; D002626:Chemistry, Pharmaceutical; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002939:Ciprofloxacin / Q000097:blood* / Q000493:pharmacokinetics / Q000592:standards; D024841:Fluoroquinolones; D006801:Humans; D015394:Molecular Structure; D015363:Quinolones / Q000737:chemistry / Q000592:standards; D015203:Reproducibility of Results
D000890:Anti-Infective Agents; D024841:Fluoroquinolones; D015363:Quinolones; D002939:Ciprofloxacin; C053091:lomefloxacin