title: Lack of effect of single high doses of buprenorphine on arterial blood gases in the rat.
abstract: High dose buprenorphine, a potent semisynthetic agonist-antagonist for opiate receptors, is now used in substitution treatment of human heroin addiction. Deaths have been reported in addicts misusing buprenorphine. We determined the median lethal dose (LD(50)) and studied the effects of high doses of intravenous buprenorphine on arterial blood gases in rats. Male Sprague-Dawley rats were administered buprenorphine intravenously to determine the LD(50) using the up-and-down method. Subsequently, catheterized groups of 10 restrained rats received no drug, saline, acid-alcohol aqueous solvent (required to dissolve buprenorphine at a high concentration), or 3, 30, or 90 mg/kg of buprenorphine intravenously. Serial arterial blood gases were obtained over 3 h. The LD(50) determined in triplicate was 146.5 mg/kg (median of 3 series, range: 142.6-176.5). The mean dose received by surviving animals was 96.9 +/- 46.7 mg/kg. There was a significant effect of the acid-alcohol aqueous solvent on arterial blood gases. Excluding the solvent effect, 3, 30, and 90-mg/kg buprenorphine doses had no significant effects on arterial blood gases. The toxicity of intravenous buprenorphine in adult rats, assessed by the LD(50), is low. These data are consistent with a wide margin of safety of buprenorphine. The mechanism of death after the intravenous administration of a lethal dose of buprenorphine remains to be determined.
authors.affiliation: INSERM U26, Université Paris VII, Hôpital Fernand Widal, 75475 Paris, France. papa.gueye@inserm.lrb.ap-hop-paris.fr
authors.forename: P N
authors.initials: PN
authors.lastname: Gueye
authors.forename: S W
authors.initials: SW
authors.lastname: Borron
authors.forename: P
authors.initials: P
authors.lastname: Risède
authors.forename: C
authors.initials: C
authors.lastname: Monier
authors.forename: F
authors.initials: F
authors.lastname: Buneaux
authors.forename: M
authors.initials: M
authors.lastname: Debray
authors.forename: F J
authors.initials: FJ
authors.lastname: Baud
mesh_terms: D000818:Animals; D001784:Blood Gas Analysis; D002047:Buprenorphine / Q000633:toxicity*; D002245:Carbon Dioxide / Q000097:blood*; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D005263:Femoral Artery; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D007275:Injections, Intravenous; D008297:Male; D009292:Narcotic Antagonists / Q000633:toxicity*; D019055:No-Observed-Adverse-Effect Level; D010100:Oxygen / Q000097:blood*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012997:Solvents / Q000494:pharmacology; D013997:Time Factors
chemical_list: D009292:Narcotic Antagonists; D012997:Solvents; D002245:Carbon Dioxide; D002047:Buprenorphine; D010100:Oxygen