Five-color-based high-information-content fingerprinting of bacterial artificial chromosome clones using type IIS restriction endonucleases.
We have developed a high-information-content fingerprinting (HICF) system for bacterial artificial chromosome (BAC) clones using a Type IIS restriction endonuclease, HgaI, paired with a Type II restriction endonuclease, RsaI. In the method described, unknown five-base overhangs generated with HgaI are partially or fully sequenced by modified fluorescent dideoxy terminators. Using an in-lane size standard labeled with a fifth dye, fragments are characterized by both the size and the sequence of its terminal one to five bases. The enhanced information content associated with this approach significantly increases the accuracy and efficiency of detecting shared fragments among BAC clones. We have compared data obtained from this method to predicted HICF patterns of 10 fully sequenced BACs. We have further applied HICF to 555 BAC clones to assemble contigs spanning 16p11.2 to 16p13.1 of human chromosome 16.
Ding
Y
Y
Beckman Institute, Division of Biology, California Institute of Technology, Pasadena, California 91125, USA.
Johnson
M D
MD
Chen
W Q
WQ
Wong
D
D
Chen
Y J
YJ
Benson
S C
SC
Lam
J Y
JY
Kim
Y M
YM
Shizuya
H
H
D022202:Chromosomes, Artificial, Bacterial; D002885:Chromosomes, Human, Pair 16; D020451:Contig Mapping; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D006801:Humans; D008957:Models, Genetic; D017422:Sequence Analysis, DNA / Q000379:methods*
D004262:DNA Restriction Enzymes