title: Inhibitory effect of lactoperoxidase-generated hypothiocyanite upon black pigmented anaerobe growth.
abstract: This study aimed to evaluate the in vitro effect of lactoperoxidase with or without its substrates (hydrogen peroxide, thiocyanate) on the growth of 4 different black pigmented anaerobe (BPA) strains associated with the development and progress of periodontal diseases: Porphyromonas gingivalis ATCC 33277, Prevotella intermedia NCTC 9336, Prevotella loescheii ATCC 15930, and Prevotella melaninogenica NCTC 9338. A 5-min lactoperoxidase-generated OSCN--HOSCN incubation at pH 6.0, 7.0 or 8.0 resulted in a decrease of the growth rate (tested by turbidimetry in liquid cultures) of the 4 BPA strains, whilst lactoperoxidase alone actually promoted bacterial growth.
authors.affiliation: Laboratory of Experimental Medicine, Free University of Brussels, Brussels, Belgium.
authors.forename: M
authors.initials: M
authors.lastname: Fadel
authors.forename: P
authors.initials: P
authors.lastname: Courtois
mesh_terms: D000704:Analysis of Variance; D002455:Cell Division / Q000187:drug effects; D005949:Glucose Oxidase / Q000378:metabolism / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007784:Lactoperoxidase / Q000378:metabolism / Q000494:pharmacology*; D016966:Porphyromonas gingivalis / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D018720:Prevotella / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D001443:Prevotella melaninogenica / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D013861:Thiocyanates / Q000378:metabolism / Q000494:pharmacology*; D013997:Time Factors
chemical_list: D013861:Thiocyanates; C014607:hypothiocyanite ion; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D007784:Lactoperoxidase; C031760:thiocyanate