Differentiation in chronic myelogenous leukemia cell K562 by spongean sesterterpene.
Scalarane-type sesterterpenes, PHC-1-PHC-7, which have been isolated from a marine sponge, increased hemoglobin production in human chronic myelogenous leukemia cell line K562 at the concentration of 0.1-5 microg/ml. PHC-1, the major constituent, induced the expression of glycophorin A and the enucleation for K562 cells. These sesterterpenes were found to induce erythroid differentiation in K562 cells. In addition, PHC-1 induced G1 arrest of the cell cycle of K562 cells.
Aoki
S
S
Graduate School of Pharmaceutical Sciences, Osaka University, Yamada-oka 1-6, Osaka, Suita, 565-0871, Japan.
Higuchi
K
K
Isozumi
N
N
Matsui
K
K
Miyamoto
Y
Y
Itoh
N
N
Tanaka
K
K
Kobayashi
M
M
D000818:Animals; D002453:Cell Cycle / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002467:Cell Nucleus / Q000187:drug effects; D004920:Erythropoiesis / Q000187:drug effects; D006021:Glycophorins / Q000378:metabolism; D006454:Hemoglobins / Q000096:biosynthesis; D006801:Humans; D020014:K562 Cells; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D011161:Porifera / Q000737:chemistry; D054830:Sesterterpenes; D013729:Terpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
D006021:Glycophorins; D006454:Hemoglobins; D054830:Sesterterpenes; D013729:Terpenes