title: A slow outward current and a hypoosmolality induced anion conductance in embryonic chicken osteoclasts.
abstract: In this paper we report on a hypoosmolality induced current, I(osmo), in embryonic chicken osteoclasts, which could only be studied when blocking a simultaneously active, unidentified slow outward current, I(slo). I(slo) was observed in all of the examined cells when both the intracellular and extracellular solutions contained sodium as the major cation and no potassium. The current was outwardly rectifying and activated at membrane potentials more positive than -44 +/- 12 mV (n = 31). The time to half activation of the current was also voltage dependent and was 350 ms at Vm = +80 mV, and 78 ms at Vm = +120 mV. The current did not inactivate during periods up to 5 s. Extracellular 4-AP (5 mM), TEA (5 mM) and Ba2+ (1 mM), blockers of K+ conductances in chicken osteoclasts, did not influence I(slo). However, I(slo) was inhibited by 50 microM extracellular verapamil, which allowed us to study I(osmo) in isolation. Exposure of the osteoclasts to hypotonic solution resulted in the development of a depolarization activated I(osmo). It developed after a 1-min delay and reached its maximum within 10 minutes. Half-maximal activation occurred after 4.4 +/- 0.9 min (n = 9). The current activated within a few ms upon depolarization and did not inactivate during at least 5 sec. I(osmo) reversed around the calculated Nernst potential for Cl- (E(Cl) = +7.3 mV and V(rev) = +5.4 +/- 3.6 mV, n = 9). The underlying conductance, G(osmo) exhibited moderate outward rectification around 0 mV in symmetrical Cl- solutions. Ion substitution experiments showed that G(osmo) is an anion conductance with P(Cl) approximately = P(F) > P(gluc) >> P(Na). I(osmo) was blocked by 0.5 mM SITS but 50 microM verapamil, 5 mM TEA, 5 mM 4-AP, 1 mM Ba2+, 50 microM cytochalasin D and 0.5 mM alendronate did not have any effect on the current. Cl- currents have been implicated in charge neutralization during osteoclastic acid secretion for bone resorption. The present results imply that osmolality may be a factor controlling this charge neutralization.
authors.affiliation: Department of Biophysics and Cell Biology, Faculty of Medicine, Medical and Health Science Center, University of Debrecen, Hungary. krasznai@jaguar.dote.hu
authors.forename: Z
authors.initials: Z
authors.lastname: Krasznai
authors.forename: F
authors.initials: F
authors.lastname: Weidema
authors.forename: D L
authors.initials: DL
authors.lastname: Ypey
authors.forename: S
authors.initials: S
authors.lastname: Damjanovich
authors.forename: R
authors.initials: R
authors.lastname: Gáspár
authors.forename: T
authors.initials: T
authors.lastname: Márián
mesh_terms: D000818:Animals; D000838:Anions; D002121:Calcium Channel Blockers / Q000494:pharmacology; D002642:Chick Embryo; D015638:Cytochalasin D / Q000494:pharmacology; D017136:Ion Transport; D008564:Membrane Potentials / Q000187:drug effects; D009994:Osmolar Concentration; D010010:Osteoclasts / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D011188:Potassium / Q000378:metabolism; D012964:Sodium / Q000378:metabolism; D014700:Verapamil / Q000494:pharmacology
chemical_list: D000838:Anions; D002121:Calcium Channel Blockers; D015638:Cytochalasin D; D012964:Sodium; D014700:Verapamil; D011188:Potassium