title: Characterization of  glutathione amide reductase from Chromatium gracile. Identification of a novel thiol peroxidase (Prx/Grx) fueled by glutathione amide redox cycling.
abstract: Among the Chromatiaceae, the glutathione derivative gamma-l-glutamyl-l-cysteinylglycine amide, or glutathione amide, was reported to be present in facultative aerobic as well as in strictly anaerobic species. The gene (garB) encoding the central enzyme in glutathione amide cycling, glutathione amide reductase (GAR), has been isolated from Chromatium gracile, and its genomic organization has been examined. The garB gene is immediately preceded by an open reading frame encoding a novel 27.5-kDa chimeric enzyme composed of one N-terminal peroxiredoxin-like domain followed by a glutaredoxin-like C terminus. The 27.5-kDa enzyme was established in vitro to be a glutathione amide-dependent peroxidase, being the first example of a prokaryotic low molecular mass thiol-dependent peroxidase. Amino acid sequence alignment of GAR with the functionally homologous glutathione and trypanothione reductases emphasizes the conservation of the catalytically important redox-active disulfide and of regions involved in binding the FAD prosthetic group and the substrates glutathione amide disulfide and NADH. By establishing Michaelis constants of 97 and 13.2 microm for glutathione amide disulfide and NADH, respectively (in contrast to K(m) values of 6.9 mm for glutathione disulfide and 1.98 mm for NADPH), the exclusive substrate specificities of GAR have been documented. Specificity for the amidated disulfide cofactor partly can be explained by the substitution of Arg-37, shown by x-ray crystallographic data of the human glutathione reductase to hydrogen-bond one of the glutathione glycyl carboxylates, by the negatively charged Glu-21. On the other hand, the preference for the unusual electron donor, to some extent, has to rely on the substitution of the basic residues Arg-218, His-219, and Arg-224, which have been shown to interact in the human enzyme with the NADPH 2'-phosphate group, by Leu-197, Glu-198, and Phe-203. We suggest GAR to be the newest member of the class I flavoprotein disulfide reductase family of oxidoreductases.
authors.affiliation: Laboratory of Protein Biochemistry and Protein Engineering, Gent University, 9000 Gent, Belgium.
authors.forename: B
authors.initials: B
authors.lastname: Vergauwen
authors.forename: F
authors.initials: F
authors.lastname: Pauwels
authors.forename: F
authors.initials: F
authors.lastname: Jacquemotte
authors.forename: T E
authors.initials: TE
authors.lastname: Meyer
authors.forename: M A
authors.initials: MA
authors.lastname: Cusanovich
authors.forename: R G
authors.initials: RG
authors.lastname: Bartsch
authors.forename: J J
authors.initials: JJ
authors.lastname: Van Beeumen
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D001483:Base Sequence; D002844:Chromatium / Q000201:enzymology* / Q000235:genetics*; D004912:Erythrocytes / Q000201:enzymology; D004926:Escherichia coli / Q000201:enzymology; D005798:Genes, Bacterial; D054477:Glutaredoxins; D005978:Glutathione / Q000031:analogs & derivatives / Q000378:metabolism*; D005980:Glutathione Reductase / Q000737:chemistry; D006801:Humans; D007700:Kinetics; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010084:Oxidation-Reduction; D010088:Oxidoreductases; D010544:Peroxidases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D011506:Proteins / Q000737:chemistry; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000737:chemistry; D015183:Restriction Mapping; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity
chemical_list: D001426:Bacterial Proteins; C516005:GLRX protein, human; D054477:Glutaredoxins; D011506:Proteins; D011993:Recombinant Fusion Proteins; C101019:glutathione amide; D010088:Oxidoreductases; D010544:Peroxidases; C430873:garB protein, Marichromatium gracile; D005980:Glutathione Reductase; D005978:Glutathione