Multi-residue analysis of avermectins in swine liver by immunoaffinity extraction and liquid chromatography-mass spectrometry.
A multi-residue LC-MS method was developed to determine avermectin residues in swine liver. Abamectin and ivermectin (22,23-dihydroabamectin) were extracted, and cleaned up by immunoaffinty columns with immobilized anti-avermectin polyclonal antibodies. The cleaned samples were separated by high-performance liquid chromatography (HPLC) with a C8 column and determined by negative-ion atmospheric pressure chemical ionization (APCI) mass spectrometry (MS) using selective ion monitoring (SIM) of [M-H]-. Recoveries of abamectin and ivermectin from fortified samples at 5-100 microg kg(-1) levels ranged from 74 to 94% and from 65 to 87%, respectively. The limits of detection were 5 microg of abamectin or ivermectin in 1 kg sample.
Wu
Z
Z
College of Chemistry and Molecular Engineering, Peking University, Beijing, China.
Li
J
J
Zhu
L
L
Luo
H
H
Xu
X
X
D000818:Animals; D000871:Anthelmintics / Q000032:analysis* / Q000737:chemistry; D000981:Antiprotozoal Agents / Q000032:analysis* / Q000737:chemistry; D002846:Chromatography, Affinity; D002853:Chromatography, Liquid / Q000379:methods; D007164:Immunosorbents; D007559:Ivermectin / Q000031:analogs & derivatives / Q000032:analysis* / Q000737:chemistry; D008099:Liver / Q000737:chemistry* / Q000378:metabolism; D013058:Mass Spectrometry; D015394:Molecular Structure; D012680:Sensitivity and Specificity; D013552:Swine
D000871:Anthelmintics; D000981:Antiprotozoal Agents; D007164:Immunosorbents; D007559:Ivermectin; C019264:avermectin