Phosphate decreases osteoclastogenesis in coculture of osteoblast and bone marrow.
Alkaline phosphatase (ALP) activity increases dramatically during osteoblast maturation, a phenomenon that is related to calcification of teeth and bone. Although the relation between ALP and calcification is widely known, there is a paucity of date relating ALP and osteoclast formation. Very recently, we showed that osteoblast maturation suppresses osteoclast formation. However, the relation between osteoblast maturation and osteoclastogenesis remains unclear. In the present study, we examined the effect of extracellular phosphate on osteoclastogenesis. As osteoblasts matured, osteoclast formation decreased, and ALP activity and inorganic phosphate in extracellular matrix increased. Inorganic phosphate in extracellular matrix and extracellular phosphate was suppressed osteoclast formation. These results suggest that phosphate released by ALP may be related not only to calcification but also to suppression of osteoclastogenesis.
Takeyama
S
S
Oral Medicine and Diagnosis, Department of Oral Functional Science, Graduate School of Dental Medicine, Hokkaido University, Kita 13 Nishi 7, Kika-ku, Hokkaido, Sapporo, 060-8586, Japan.
Yoshimura
Y
Y
Deyama
Y
Y
Sugawara
Y
Y
Fukuda
H
H
Matsumoto
A
A
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000378:metabolism; D016723:Bone Remodeling / Q000187:drug effects / Q000502:physiology; D002113:Calcification, Physiologic; D002450:Cell Communication; D002454:Cell Differentiation; D002460:Cell Line; D018920:Coculture Techniques; D005109:Extracellular Matrix / Q000378:metabolism; D051379:Mice; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism; D010010:Osteoclasts / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism / Q000494:pharmacology*
D010710:Phosphates; D000469:Alkaline Phosphatase