Brownian dynamics simulations of fluorescence fluctuation spectroscopy.
We have developed a program for the simulation of the fluorescence fluctuations as detected from highly diluted samples of (bio)molecules. The model is applied to translational diffusion and takes into account the hydrodynamic interactions. The solution concentration is kept constant by assuming periodic boundary conditions and spans here the range 0.5< C < 10 nM. We show that the fluorescence correlation functions can be accurately computed on systems of limited size (a few molecules per simulation box) by simulating for a total time approximately 100-300 times the diffusion relaxation time of the fluorescence autocorrelation function. The model is applied also to the simulation of the scanning fluorescence correlation spectroscopy (FCS) and of the photon counting histograms for the confocal collection configuration. Scanning FCS simulations of highly diluted samples (C approximately equals 0.5 nM) show anticorrelation effects in the autocorrelation functions of the fluorescence signal that are less evident for higher concentrations. We suggest here that this effect may be due to the non-uniform occupancy of the scanning area by the fluorophores.
Huertas de la Torre
M
M
Dipartimento di Fisica G. Occhialini, Università di Milano Bicocca, Italy.
Forni
R
R
Chirico
G
G
D000465:Algorithms; D001703:Biophysics / Q000379:methods; D003198:Computer Simulation; D004058:Diffusion; D007700:Kinetics; D017785:Photons; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry*; D012996:Solutions; D013050:Spectrometry, Fluorescence / Q000379:methods*
D011506:Proteins; D012996:Solutions