title: An essential role of the NF-kappa B/Toll-like receptor pathway in induction of inflammatory and tissue-repair gene expression by necrotic cells.
abstract: Tissue damage induced by infection or injury can result in necrosis, a mode of cell death characterized by induction of an inflammatory response. In contrast, cells dying by apoptosis do not induce inflammation. However, the reasons for underlying differences between these two modes of cell death in inducing inflammation are not known. Here we show that necrotic cells, but not apoptotic cells, activate NF-kappaB and induce expression of genes involved in inflammatory and tissue-repair responses, including neutrophil-specific chemokine genes KC and macrophage-inflammatory protein-2, in viable fibroblasts and macrophages. Intriguingly, NF-kappaB activation by necrotic cells was dependent on Toll-like receptor 2, a signaling pathway that induces inflammation in response to microbial agents. These results have identified a novel mechanism by which cell necrosis, but not apoptosis, can induce expression of genes involved in inflammation and tissue-repair responses. Furthermore, these results also demonstrate that the NF-kappaB/Toll-like receptor 2 pathway can be activated both by exogenous microbial agents and endogenous inflammatory stimuli.
authors.affiliation: Department of Biological Sciences, Columbia University, New York, NY 10027, USA.
authors.forename: M
authors.initials: M
authors.lastname: Li
authors.forename: D F
authors.initials: DF
authors.lastname: Carpio
authors.forename: Y
authors.initials: Y
authors.lastname: Zheng
authors.forename: P
authors.initials: P
authors.lastname: Bruzzo
authors.forename: V
authors.initials: V
authors.lastname: Singh
authors.forename: F
authors.initials: F
authors.lastname: Ouaaz
authors.forename: R M
authors.initials: RM
authors.lastname: Medzhitov
authors.forename: A A
authors.initials: AA
authors.lastname: Beg
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics / Q000276:immunology; D002460:Cell Line; D002478:Cells, Cultured; D054360:Chemokine CXCL1; D018925:Chemokines; D019743:Chemokines, CXC; D016207:Cytokines / Q000096:biosynthesis; D029721:Drosophila Proteins; D004622:Embryo, Mammalian; D005347:Fibroblasts / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D005786:Gene Expression Regulation / Q000276:immunology*; D007249:Inflammation / Q000235:genetics / Q000276:immunology / Q000473:pathology; D018836:Inflammation Mediators / Q000378:metabolism; D007375:Interleukin-1 / Q000502:physiology; D016209:Interleukin-8 / Q000096:biosynthesis; D008264:Macrophages / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D008562:Membrane Glycoproteins / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D016328:NF-kappa B / Q000378:metabolism / Q000502:physiology*; D009336:Necrosis; D011956:Receptors, Cell Surface / Q000502:physiology*; D015398:Signal Transduction / Q000276:immunology; D051195:Toll-Like Receptor 2; D051193:Toll-Like Receptors; D051996:Transcription Factor RelA; D014945:Wound Healing / Q000235:genetics* / Q000276:immunology*
chemical_list: D054360:Chemokine CXCL1; D018925:Chemokines; D019743:Chemokines, CXC; C475895:Cxcl1 protein, mouse; D016207:Cytokines; D029721:Drosophila Proteins; D018836:Inflammation Mediators; D007375:Interleukin-1; D016209:Interleukin-8; D008562:Membrane Glycoproteins; D016328:NF-kappa B; D011956:Receptors, Cell Surface; D051195:Toll-Like Receptor 2; D051193:Toll-Like Receptors; D051996:Transcription Factor RelA; C074302:keratinocyte-derived chemokines