Analysis of thiocyanate in biological fluids by capillary zone electrophoresis.
A new sensitive and simple method has been developed for the determination of thiocyanate in human serum, urine and saliva. The determinations were performed in a fused-silica capillary [64.5 cm (56 cm effective length) x 75 microm] using 0.1 M beta-alanine-HCl (pH 3.50) as a background electrolyte, separation voltage 18 kV (negative polarity), temperature of capillary 25 degrees C and direct detection at 200 nm. Serum samples were 10-times diluted with deionised water and deproteinised with acetonitrile in the ratio 1:2. Urine and saliva samples need only 20-fold dilution with deionised water. The proposed method was successfully applied to the determination of thiocyanate in various human serum, saliva and urine samples.
Glatz
Z
Z
Department of Biochemistry, Faculty of Science, Masaryk University, Brno, Czech Republic. glatz@chemi.muni.cz
Nováková
S
S
Sterbová
H
H
D019075:Electrophoresis, Capillary / Q000379:methods*; D015203:Reproducibility of Results; D012463:Saliva / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D013861:Thiocyanates / Q000032:analysis* / Q000097:blood / Q000652:urine
D013861:Thiocyanates