A comparison of the induction of immortalized endothelial cell impermeability by astrocytes.
The suitability of various commercially available endothelial cell lines in studies of astrocytic/endothelial cell interactions was assessed. The endothelial-like cell line ECV304 was compared with T24/83, Eahy929, and b.End5 and rat cerebral endothelial cells in their ability, when co-cultured with rat (C6) glioma cells, to form a transendothelial electrical resistance (TEER), an indicator of tight junction formation which is an important property of the blood-brain barrier. As reported previously, the basal TEER of ECV304 cell monolayers was significantly enhanced upon co-culture, an effect reproduced by human 1321N1 astrocytes and primary rat astrocytes. T24/83 cells formed a patchy, gapped monolayer, which produced a poor basal TEER with little in the way of an increase upon co-culture. Similarly, all the other cell monolayers analysed demonstrated poor TEERs that were only moderately increased upon co-culture. These data confirm that while no endothelial cell line with ideal features is available, ECV304 cells remain an appropriate choice especially for studies of astrocyte/endothelial cell interactions.
Tan
K H
KH
Centre for Research in Biomedicine, Faculty of Applied Sciences, University of the West of England, Bristol, UK.
Dobbie
M S
MS
Felix
R A
RA
Barrand
M A
MA
Hurst
R D
RD
D000818:Animals; D001253:Astrocytes / Q000166:cytology* / Q000378:metabolism; D001812:Blood-Brain Barrier / Q000502:physiology*; D002450:Cell Communication / Q000502:physiology*; D002461:Cell Line, Transformed / Q000166:cytology* / Q000378:metabolism; D002463:Cell Membrane Permeability / Q000502:physiology*; D018920:Coculture Techniques; D003470:Culture Media / Q000494:pharmacology; D017097:Electric Impedance; D004558:Electric Stimulation; D004730:Endothelium, Vascular / Q000166:cytology* / Q000378:metabolism; D005333:Fetus; D006801:Humans; D008564:Membrane Potentials / Q000502:physiology; D051379:Mice; D008954:Models, Biological; D051381:Rats; D019108:Tight Junctions / Q000378:metabolism
D003470:Culture Media