title: Intracellular electrical activity in human urinary bladder smooth muscle: the effect of high sucrose medium.
abstract: BACKGROUND
  The primary key to pharmacotherapy of bladder instability is in the excitation-contraction coupling of detrusor smooth muscle cells. To study this process, simultaneous recordings of mechanical and electrical activity are required. However, recording of mechanical activity induces movement, which may affect the quality of intracellular recordings.
  
  
  METHODS
  We therefore compared the electrical activity of human detrusor smooth muscle cells in normal Krebs' solution and in a hypertonic solution, which immobilizes the tissue, enabling us to study the effect of movement on the membrane potential. Carbachol and KCl were applied to induce contractions.
  
  
  RESULTS
  Sucrose in the medium made the tissue rigid and abolished its movement, while the electrical response was not affected. When compared with recordings in normal Krebs' solution, the average resting membrane potential was not altered. However, the membrane potential was more stable, with far less spike-shaped potentials. The spike-shaped potential amplitude was larger, while the duration was decreased.
  
  
  CONCLUSIONS
  Impairing the ability of tissue movement resulted in changes in the electrophysiological properties of detrusor smooth muscle cells. The results suggest that stretch has an effect on L-type Ca2+ channels.
authors.affiliation: Department of Urology-Urodynamics, Erasmus University Rotterdam, The Netherlands.
authors.forename: A J
authors.initials: AJ
authors.lastname: Visser
authors.forename: R
authors.initials: R
authors.lastname: van Mastrigt
mesh_terms: D000200:Action Potentials / Q000187:drug effects*; D000368:Aged; D000369:Aged, 80 and over; D002478:Cells, Cultured; D003470:Culture Media / Q000494:pharmacology; D004594:Electrophysiology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009130:Muscle, Smooth / Q000502:physiology*; D013395:Sucrose / Q000494:pharmacology*; D001743:Urinary Bladder / Q000502:physiology*
chemical_list: D003470:Culture Media; D013395:Sucrose