Evidence that RME-1, a conserved C. elegans EH-domain protein, functions in endocytic recycling.
In genetic screens for new endocytosis genes in Caenorhabditis elegans we identified RME-1, a member of a conserved class of Eps15-homology (EH)-domain proteins. Here we show that RME-1 is associated with the periphery of endocytic organelles, which is consistent with a direct role in endocytic transport. Endocytic defects in rme-1 mutants indicate that the protein is likely to have a function in endocytic recycling. Evidence from studies of mammalian RME-1 also points to a function for RME-1 in recycling, specifically in the exit of membrane proteins from recycling endosomes. These studies show a conserved function in endocytic recycling for the RME-1 family of EH proteins.
Grant
B
B
Columbia University College of Physicians and Surgeons, Department of Biochemistry and Molecular Biophysics, 630 West 168th Street, New York, New York 10032, USA. grant@cuccfa.ccc.columbia.edu
Zhang
Y
Y
Paupard
M C
MC
Lin
S X
SX
Hall
D H
DH
Hirsh
D
D
D000818:Animals; D017173:Caenorhabditis elegans / Q000502:physiology*; D029742:Caenorhabditis elegans Proteins; D002135:Calcium-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D017124:Conserved Sequence; D004705:Endocytosis / Q000235:genetics; D011992:Endosomes / Q000502:physiology; D004848:Epithelium / Q000502:physiology; D008969:Molecular Sequence Data; D009154:Mutation; D010750:Phosphoproteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D017434:Protein Structure, Tertiary; D022161:Transport Vesicles / Q000502:physiology*
D029742:Caenorhabditis elegans Proteins; D002135:Calcium-Binding Proteins; D010750:Phosphoproteins; C486205:RME-1 protein, C elegans