title: Evaluation of formalin-fixed paraffin-embedded tissues from vaccine site-associated sarcomas of cats for polyomavirus DNA and antigen.
abstract: OBJECTIVE
  To determine whether vaccine site-associated sarcomas (VSS) from cats contain polyomavirus antigen or DNA.
  
  
  METHODS
  50 formalin-fixed paraffin-embedded tissue blocks of VSS from cats.
  
  
  METHODS
  Sections from each tissue block were evaluated for polyomavirus antigen by use of an avidin-biotin-complex immunohistochemical staining method, using rabbit anti-murine polyomavirus polyclonal antiserum as the primary antibody. The DNA was extracted from sections of each tissue block, and a polymerase chain reaction assay was performed, using primers designed to amplify regions of the bovine polyomavirus genome and consensus polyomavirus primers designed to detect unknown polyomaviruses.
  
  
  RESULTS
  Polyomavirus antigen and DNA were not detected in any of the VSS.
  
  
  CONCLUSIONS
  Results suggest that polyomaviruses likely do not have any direct involvement in the pathogenesis of VSS in cats.
authors.affiliation: Department of Veterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Canada.
authors.forename: B A
authors.initials: BA
authors.lastname: Kidney
authors.forename: D M
authors.initials: DM
authors.lastname: Haines
authors.forename: J A
authors.initials: JA
authors.lastname: Ellis
authors.forename: M
authors.initials: M
authors.lastname: Burnham
authors.forename: M L
authors.initials: ML
authors.lastname: Jackson
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D002371:Cat Diseases / Q000276:immunology / Q000473:pathology / Q000821:virology*; D002415:Cats; D004279:DNA, Viral / Q000032:analysis*; D005354:Fibrosarcoma / Q000276:immunology / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D005557:Formaldehyde; D007150:Immunohistochemistry / Q000662:veterinary; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011120:Polyomavirus / Q000235:genetics / Q000276:immunology; D017422:Sequence Analysis, DNA; D016707:Tissue Fixation / Q000662:veterinary; D014611:Vaccination / Q000009:adverse effects / Q000662:veterinary*; D014765:Viral Vaccines / Q000009:adverse effects* / Q000235:genetics / Q000276:immunology
chemical_list: D000956:Antigens, Viral; D004279:DNA, Viral; D014765:Viral Vaccines; D005557:Formaldehyde