title: Chemorepellent signaling through the PACAP/lysozyme receptor is mediated through cAMP and PKC in Tetrahymena thermophila.
abstract: Pituitary adenylate cyclase-activating peptide and lysozyme are potent chemorepellents which act through the same receptor in Tetrahymena. Using in vivo behavioral studies, we have found that the pituitary adenylate cyclase-activating peptide/lysozyme receptor appears to signal through a G-protein pathway which is mediated through both adenosine 3'5'monophosphate and protein kinase C. Avoidance to pituitary adenylate cyclase-activating peptide and lysozyme is inhibited by the G-protein inhibitor, guanosine 5'-O-(2thiodiphosphate), the adenosine 3'5'monophate analog, Rp-adenosine-3', 5' cyclic monophosphorothioate, and the protein kinase C inhibitors, calphostin C and bisindolylmaleimide IV. A proposed model for signaling through the pituitary adenylate cyclase-activating peptide/lysozyme receptor is briefly outlined.
authors.affiliation: Cedarville University, Department of Science and Mathematics, OH 45314, USA.
authors.forename: D L
authors.initials: DL
authors.lastname: Hassenzahl
authors.forename: N K
authors.initials: NK
authors.lastname: Yorgey
authors.forename: M D
authors.initials: MD
authors.lastname: Keedy
authors.forename: A R
authors.initials: AR
authors.lastname: Price
authors.forename: J A
authors.initials: JA
authors.lastname: Hall
authors.forename: C C
authors.initials: CC
authors.lastname: Myzcka
authors.forename: H G
authors.initials: HG
authors.lastname: Kuruvilla
mesh_terms: D000818:Animals; D001522:Behavior, Animal; D002628:Chemoreceptor Cells; D000242:Cyclic AMP / Q000494:pharmacology*; D008124:Locomotion; D009113:Muramidase / Q000494:pharmacology; D009479:Neuropeptides / Q000494:pharmacology*; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D011493:Protein Kinase C / Q000494:pharmacology*; D016808:Tetrahymena thermophila / Q000502:physiology*
chemical_list: D009479:Neuropeptides; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D000242:Cyclic AMP; D011493:Protein Kinase C; D009113:Muramidase