title: 1H NMR spectroscopic investigation of serum and urine in a case of acute tetrahydrofuran poisoning.
abstract: This article reports the investigation by 1H nuclear magnetic resonance (1H NMR) spectroscopy of biological fluids in a case of intentional poisoning with tetrahydrofuran (THF). Occupational exposures to this solvent are well documented, but acute poisoning cases are extremely rare, and the one presented here is the second known case of this kind. Urine and serum samples were collected. Without any pretreatment, the presence of THF was confirmed by characteristic resonances at 1.90 and 3.76 ppm; high lactate levels were also observed. The presence of gamma-hydroxybutyric acid (GHB) was noted. Quantitative analysis was performed by relative integration of peak areas. THF concentrations were 813 and 850 mg/L (11.3 and 11.8 mmol/L), and GHB concentrations 239 and 2,977 mg/L (2.3 and 28.6 mmol/L) in serum and urine, respectively. A gas chromatographic-mass spectrometric method confirmed 1H NMR observations. The origin of GHB detected in serum and urine is also discussed.
authors.affiliation: Laboratoire de Biochimie et de Biologie Moléculaire, Hĵpital Albert Calmette, Lille, France.
authors.forename: B
authors.initials: B
authors.lastname: Cartigny
authors.forename: N
authors.initials: N
authors.lastname: Azaroual
authors.forename: M
authors.initials: M
authors.lastname: Imbenotte
authors.forename: N
authors.initials: N
authors.lastname: Sadeg
authors.forename: F
authors.initials: F
authors.lastname: Testart
authors.forename: J
authors.initials: J
authors.lastname: Richecoeur
authors.forename: G
authors.initials: G
authors.lastname: Vermeersch
authors.forename: M
authors.initials: M
authors.lastname: Lhermitte
mesh_terms: D000208:Acute Disease; D005260:Female; D005663:Furans / Q000097:blood / Q000737:chemistry / Q000506:poisoning* / Q000652:urine; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D006885:Hydroxybutyrates / Q000097:blood / Q000652:urine; D019344:Lactic Acid / Q000097:blood / Q000652:urine; D009682:Magnetic Resonance Spectroscopy; D008875:Middle Aged; D012997:Solvents / Q000737:chemistry / Q000506:poisoning; D013057:Spectrum Analysis
chemical_list: D005663:Furans; D006885:Hydroxybutyrates; D012997:Solvents; C111420:4-hydroxybutyric acid; D019344:Lactic Acid; C018674:tetrahydrofuran