Distribution of resistance genes tet(M), aph3'-III, catpC194 and the integrase gene of Tn1545 in clinical Streptococcus pneumoniae harbouring erm(B) and mef(A) genes in Spain.
The most prevalent macrolide resistance phenotype and genotype among pneumococcal isolates was the cMLSB phenotype [erm(B) or erm(B)/mef(A)] (91.3%). We studied the distribution of other resistance genes, tet(M), catpC194, aph3'-III, in these strains, seeing evolution at work in that some strains carried different combinations of resistance determinants. The most prevalent patterns associated with resistance to erythromycin [erm(B)] were resistance to tetracycline [tet(M)] and chloramphenicol (catpC194) (48.2%) or resistance to tetracycline [tet(M)] alone (42.2%). In our isolates of Streptococcus pneumoniae there was a strong association of the erm(B) and tet(M) genes with Tn1545-related elements.
Seral
C
C
Department of Microbiology, University Hospital 'Lozano Blesa', San Juan Bosco 15, 50009 Zaragoza, Spain. c.seral.000@recol.es
Castillo
F J
FJ
Rubio-Calvo
M C
MC
Fenoll
A
A
García
C
C
Gómez-Lus
R
R
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics*; D002702:Chloramphenicol Resistance / Q000235:genetics; D003227:Conjugation, Genetic / Q000235:genetics; D004251:DNA Transposable Elements / Q000235:genetics*; D004352:Drug Resistance, Microbial / Q000235:genetics; D006801:Humans; D019426:Integrases / Q000235:genetics*; D019868:Kanamycin Kinase / Q000235:genetics*; D018942:Macrolides; D008565:Membrane Proteins / Q000378:metabolism; D008780:Methyltransferases / Q000378:metabolism; D008826:Microbial Sensitivity Tests; D013030:Spain; D013296:Streptococcus pneumoniae / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D018942:Macrolides; C117866:MefA protein, Streptococcus; D008565:Membrane Proteins; C083138:Tet M resistance protein, Bacteria; D008780:Methyltransferases; C035181:rRNA (adenosine-O-2'-)methyltransferase; D019868:Kanamycin Kinase; D019426:Integrases