title: Localization of oestrogen receptors alpha and beta in human testis.
abstract: Cellular localization of oestrogen receptor alpha (ERalpha) and beta (ERbeta) proteins were studied in human testis samples using immunohistochemistry, and the expression of the corresponding mRNA was examined with reverse transcription-polymerase chain reaction (RT-PCR). Seven men, aged 28-48 years, who underwent diagnostic testicular biopsy because of azoospermia or to give spermatozoa for intracytoplasmic injection for infertility treatment, donated tissue for the study. One of them had anejaculation but normally functioning testes, and one was diagnosed as having Sertoli cell-only syndrome (SCOS). In addition, expression of ERbeta protein was examined in one testis sample obtained from a man undergoing a sex change operation. Strong ERbeta immunoreactivity was detected in the nuclei of spermatogonia, spermatocytes and early developing spermatids. Elongating spermatids, mature spermatozoa, Sertoli and Leydig cells were all negative for ERbeta. The presence of ERbeta protein was confirmed in Western analysis. With RT-PCR, both wild-type ERbeta and ERbetacx, the isoform which represses wild-type ER function, were easily detected. In most cases, ERbetacx mRNA was more abundantly expressed than wild-type ERbeta. The patient with SCOS expressed neither ERbeta isoform. Neither ERalpha protein nor ERalpha mRNA was detected in any of the samples. We conclude that in the human testis, ERbeta is likely to be the ER that mediates the effects of oestrogen.
authors.affiliation: Karolinska Institutet, Department of Medical Nutrition, Huddinge University Hospital, S-141 86 Huddinge, Sweden.
authors.forename: S
authors.initials: S
authors.lastname: Mäkinen
authors.forename: S
authors.initials: S
authors.lastname: Mäkelä
authors.forename: Z
authors.initials: Z
authors.lastname: Weihua
authors.forename: M
authors.initials: M
authors.lastname: Warner
authors.forename: B
authors.initials: B
authors.lastname: Rosenlund
authors.forename: S
authors.initials: S
authors.lastname: Salmi
authors.forename: O
authors.initials: O
authors.lastname: Hovatta
authors.forename: J A
authors.initials: JA
authors.lastname: Gustafsson
mesh_terms: D000328:Adult; D000818:Animals; D015153:Blotting, Western / Q000379:methods; D002499:Centrifugation, Density Gradient / Q000379:methods; D003600:Cytosol / Q000737:chemistry; D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D051379:Mice; D008875:Middle Aged; D012333:RNA, Messenger; D011960:Receptors, Estrogen / Q000032:analysis* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013737:Testis / Q000737:chemistry* / Q000473:pathology
chemical_list: D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D012333:RNA, Messenger; D011960:Receptors, Estrogen