Chromatographic purification of some 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitors.
The purification of pravastatin, simvastatin and lovastatin in the sodium salt or lactone form and of mevastatin in the lactone form by reversed-phase displacement chromatography is presented. The mobile phases consisted of water or mixtures of water-methanol and water-acetonitrile. Six different displacers were successfully used. Up to 0.14 g of raw sample per gram of stationary phase was loaded on a column packed with silica-based octadecyl phase. Crude substances from 85 to 88% chromatographic purity were purified and at least 99.5% purity was achieved.
Grahek
R
R
Lek d.d., Research and Development, Ljubljana, Slovenia.
Milivojevic
D
D
Bastarda
A
A
Kracun
M
M
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors / Q000302:isolation & purification*; D008148:Lovastatin / Q000031:analogs & derivatives* / Q000302:isolation & purification*; D017035:Pravastatin / Q000302:isolation & purification*; D019821:Simvastatin / Q000302:isolation & purification*; D013056:Spectrophotometry, Ultraviolet
D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; C012258:mevastatin; D008148:Lovastatin; D019821:Simvastatin; D017035:Pravastatin