[Effect of cultivation conditions on the functional activity of thyrocytes in the organ culture of the thyroid gland in newborn piglets].
In new-born piglets' thyroid gland culture, thyrocytes were capable of synthesising thyroxine and triiodothyronine for over 12 days. A 48-hour decline of the medium pH to 6.45 did not affect further functional activity of the cells. Decrease in temperature to 26-28 degrees C augmented the thyroid hormones level as opposed to continuous cultivation at 37 degrees C. Degradation of the thyroid gland follicular structure induced a change in the hormones biosynthesis. The thyrocytes not arranged in the follicles mainly produced triiodothyronine in concentrations 5 to 8-fold higher than the T4 contents.
Nadol'nik
L I
LI
Institute of Biochemistry, National Acad. Sci., Belorussia, 230017, Grodno, BLK-50.
Basinskiĭ
V A
VA
Martynchik
D I
DI
Vinogradov
V V
VV
D000818:Animals; D000831:Animals, Newborn; D017364:Collagenases; D006863:Hydrogen-Ion Concentration; D009924:Organ Culture Techniques; D013552:Swine; D013696:Temperature; D013961:Thyroid Gland / Q000166:cytology / Q000378:metabolism / Q000502:physiology; D013974:Thyroxine / Q000096:biosynthesis; D014284:Triiodothyronine / Q000096:biosynthesis
D014284:Triiodothyronine; D017364:Collagenases; D013974:Thyroxine