Glycogen accumulation in cultured tumoral or normal pancreatic islet and acinar cells.
Tumoral pancreatic islet cells of either the RINm5F or INS-1 cell lines, when cultured in the presence of 30.0 mM D-glucose, accumulate about 50 times more glycogen than tumoral pancreatic acinar cells of the AR42J line cultured under the same experimental conditions. Expressed per nl of intracellular water, the glycogen content of the RINm5F or INS-1 cells is even higher than that found in rat pancreatic islets also cultured under the same experimental conditions. Moreover, at variance with normal islet cells, tumoral islet cells do not require to be exposed to a high concentration of D-glucose to accumulate large amounts of glycogen. Based on these findings, it is proposed that the labelling of the glycogen pool, e.g. by 11C-labelled D-glucose or 2-deoxy-2-[18F]fluoro-D-glucose, may allow identification and localization of insulinomas in the pancreatic gland by a non-invasive imaging procedure.
Rasschaert
J
J
Laboratory of Experimental Medicine, Brussels Free University, Brussels, Belgium.
Malaisse
W J
WJ
D000818:Animals; D002250:Carbon Radioisotopes; D004305:Dose-Response Relationship, Drug; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology; D006003:Glycogen / Q000378:metabolism*; D066298:In Vitro Techniques; D007515:Islets of Langerhans / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D010179:Pancreas / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000187:drug effects / Q000378:metabolism
D002250:Carbon Radioisotopes; D006003:Glycogen; D005947:Glucose