Cloning and expression pattern of the human NDRG3 gene.
We report the cloning and expression pattern of a novel N-myc downstream-regulated gene 3 (NDRG3), located on human chromosome 20q11.21-11.23. The NDRG3 cDNA is 2588 base pair in length, encoding a 363 amino acid polypeptide highly related to mouse Ndr3 protein. Northern blot reveals that NDRG3 is highly expressed in testis, prostate and ovary. By in situ hybridization, the NDRG3 mRNA was localized to the outer layers of seminiferous epithelium, indicating that it may play a role in spermatogenesis.
Zhao
W
W
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, People's Republic of China.
Tang
R
R
Huang
Y
Y
Wang
W
W
Zhou
Z
Z
Gu
S
S
Dai
J
J
Ying
K
K
Xie
Y
Y
Mao
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000096:biosynthesis* / Q000737:chemistry; D004848:Epithelium / Q000378:metabolism; D005260:Female; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins; D010053:Ovary / Q000378:metabolism; D011467:Prostate / Q000378:metabolism; D014176:Protein Biosynthesis; D011506:Proteins / Q000737:chemistry / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D016415:Sequence Alignment; D013091:Spermatogenesis; D013737:Testis / Q000378:metabolism
D018076:DNA, Complementary; D047908:Intracellular Signaling Peptides and Proteins; C431706:NDRG3 protein, human; C402397:Ndrg3 protein, mouse; D009419:Nerve Tissue Proteins; D011506:Proteins; D012333:RNA, Messenger