Intrachannel versus interchannel application of angiogenic factors in transmyocardial laser revascularization.
OBJECTIVE
Theoretically myocardial angiogenesis of laser injury can be further enhanced by the addition of angiogenic growth factors. The influence of the way of administration of these factors on vascular growth around the channels is still unclear.


METHODS
18 pigs (mean weight 72 +/- 5.2 kg) were randomized to either triads of transmyocardial laser revascularization (TMLR) channels (group 1, n = 6) or isolated channels (group 2, n = 6), or a control group (n = 6). The animals had injections of bovine bone derived growth factor mixture either in the center of the triads in group 1 or within the channels themselves in group 2. Animals were sacrificed one month later for histological analysis.


RESULTS
The vascular densities of myocardial areas within the triads of group 1 and around the channels in group 2 were significantly larger than in the control group: 15.2 +/- 3.7/mm2 and 14.2 +/- 3.5/mm2 respectively vs 5.3 +/- 1.6/mm2 (p < 0.001 for both differences). Differences of densities between group 1 and 2 were not statistically significant (p = 0.6).


CONCLUSIONS
In this porcine model, the addition of a bovine bone derived growth factor mixture to TMLR significantly stimulates angiogenesis in the areas adjacent to the channels. The place of injection does not influence the angiogenesis intensity.
Mueller
X M
XM
Clinic for Cardiovascular Surgery, CHUV (Centre Hospitalier Universitaire Vaudois), Lausanne. xavier.mueller@chuv.hospvd.ch
Tevaearai
H T
HT
Genton
C Y
CY
Chaubert
P
P
von Segesser
L K
LK
D043925:Angiogenesis Inducing Agents / Q000494:pharmacology*; D000818:Animals; D003331:Coronary Vessels / Q000187:drug effects* / Q000473:pathology; D004338:Drug Combinations; D006133:Growth Substances / Q000494:pharmacology*; D006352:Heart Ventricles / Q000473:pathology / Q000601:surgery; D007267:Injections; D053685:Laser Therapy / Q000379:methods*; D009204:Myocardial Revascularization / Q000379:methods*; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D013552:Swine
D043925:Angiogenesis Inducing Agents; D004338:Drug Combinations; D006133:Growth Substances