Enantioseparation of ofloxacin in urine by capillary electrokinetic chromatography using charged cyclodextrins as chiral selectors and assessment of enantioconversion.
A method was developed for the enantioseparation of ofloxacin, a member of the fluoroquinolones, using an anionic cyclodextrin-derivative with or without combination with a neutral cyclodextrin-derivative, as the chiral selector (s) in an electrokinetic chromatography system. The best results were obtained with 0.35 mM sulfated beta-cyclodextrin dissolved in a 50 mM phosphate buffer, pH 2.5, and at 15 degrees C. Under these conditions, a resolution of 2 was readily achieved. Furthermore, under adequate separation conditions, studies were performed in order to assess possible in vitro and in vivo enantioconversion of levofloxacin. The current method allows detection of 2 microg R-(+)-ofloxacine/mL diluted urine without the necessity of sample cleanup.
de Boer
T
T
Department of Analytical Chemistry and Toxicology, University Center for Pharmacy, Groningen, The Netherlands. t.de.boer@farm.rug.nl
Mol
R
R
de Zeeuw
R A
RA
de Jong
G J
GJ
Ensing
K
K
D002845:Chromatography / Q000379:methods; D003505:Cyclodextrins; D019075:Electrophoresis, Capillary / Q000379:methods; D006801:Humans; D064704:Levofloxacin; D015242:Ofloxacin / Q000652:urine*
D003505:Cyclodextrins; D064704:Levofloxacin; D015242:Ofloxacin