Osmotic perturbations induce differential movements in the core and periphery of proteins, membranes and micelles.
Polymeric structures, namely, micelles, membranes and globular proteins share the property of two distinct regions: a hydrophobic core and a hydrophilic exterior. The dynamics of these regions of the polymeric structures were probed using selective fluorophores 1,6-diphenyl-1,3,5-hexatriene (DPH) and 1-anilinonaphthalene-8-sulfonate (ANS), respectively. Perturbation of the polymers by external osmotic pressure, ionic strength and temperature was monitored in the two regions using steady state measurements of fluorescence intensity and anisotropy. While the fluorescence lifetime of DPH and ANS did not change significantly, parallel change in steady state anisotropy values and the rotational correlation time indicated mobility in the probe/probe-domain. Osmotic perturbation of the polymers in electrolyte media led to decreased DPH mobility. Enhanced ellipticity at 222 nm in bovine serum albumin was observed in 1.5 M NaCl and sucrose media. ANS exhibited a decreased anisotropy with progressive dehydration in proteins in NaCl media, in dimyristoylphosphatidylcholine (DMPC) vesicles in sucrose media, and in neutral laurylmaltoside micelles in both NaCl and sucrose media. Thus, ANS showed responses opposite to that of DPH in these systems. A comparison with several domain selective probes indicated that DPH reported findings common to depth probes while ANS reported data common to interfacial probes used for voltage monitoring.
Madhavarao
C N
CN
Department of Biotechnology, University of Pune, India.
Sauna
Z E
ZE
Srivastava
A
A
Sitaramam
V
V
D000817:Anilino Naphthalenesulfonates; D002462:Cell Membrane / Q000737:chemistry; D002942:Circular Dichroism; D004161:Diphenylhexatriene; D005456:Fluorescent Dyes; D008823:Micelles; D009997:Osmotic Pressure; D011506:Proteins / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
D000817:Anilino Naphthalenesulfonates; D005456:Fluorescent Dyes; D008823:Micelles; D011506:Proteins; D004161:Diphenylhexatriene; C027132:1-anilino-8-naphthalenesulfonate