title: Enzymatic computing.
abstract: The conformational dynamics of enzymes is a computational resource that fuses milieu signals in a nonlinear fashion. Response surface methodology can be used to elicit computational functionality from enzyme dynamics. We constructed a tabletop prototype to implement enzymatic signal processing in a device context and employed it in conjunction with malate dehydrogenase to perform the linearly inseparable exclusive-or operation. This shows that proteins can execute signal processing operations that are more complex than those performed by individual threshold elements. We view the experiments reported, though restricted to the two-variable case, as a stepping stone to computational networks that utilize the precise reproducibility of proteins, and the concomitant reproducibility of their nonlinear dynamics, to implement complex pattern transformations.
authors.affiliation: Department of Computer Science, Wayne State University, Detroit, Michigan 48202, USA.
authors.forename: K P
authors.initials: KP
authors.lastname: Zauner
authors.forename: M
authors.initials: M
authors.lastname: Conrad
mesh_terms: D002118:Calcium / Q000737:chemistry / Q000378:metabolism; D004798:Enzymes / Q000737:chemistry* / Q000378:metabolism*; D007091:Image Processing, Computer-Assisted; D008274:Magnesium / Q000737:chemistry / Q000378:metabolism; D008291:Malate Dehydrogenase / Q000737:chemistry / Q000378:metabolism; D008956:Models, Chemical; D008958:Models, Molecular; D009994:Osmolar Concentration; D011487:Protein Conformation
chemical_list: D004798:Enzymes; D008291:Malate Dehydrogenase; D008274:Magnesium; D002118:Calcium