Reversed-phase planar chromatography of some enantiomeric amino acids and oxazolidinones.
The resolution of two enantiomeric amino acids and four structurally related oxazolidinones was evaluated by reversed-phase planar chromatography using both home-made microcrystalline cellulose triacetate (MCTA) layers and mobile phase additivies, such as beta-cyclodextrins (beta-Cy), modified alpha, beta, gamma cyclodextrins and bovine serum albumin (BSA), on commercially available RP-18W/F254 and SilC18-50/UV254 plates. All the enantiomers were partially or baseline resolved by at least one of the chiral phases tested. Densitograms of optical antipodes and their mixtures were measured on MCTA layers developed with hydroalcoholic solvents.
Lepri
L
L
Department of Public Health, Epidemiology and Environmental Analytical Chemistry, University of Florence, Florence, Italy. lepri@dsp.igiene.unfi.it
Boddi
L
L
Bubba
M D
MD
Cincinelli
A
A
D000596:Amino Acids / Q000737:chemistry / Q000302:isolation & purification*; D002482:Cellulose / Q000031:analogs & derivatives*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003460:Crystallization; D003505:Cyclodextrins; D007202:Indicators and Reagents; D008567:Membranes, Artificial; D023303:Oxazolidinones / Q000737:chemistry / Q000302:isolation & purification*; D012710:Serum Albumin, Bovine; D013237:Stereoisomerism; D047392:beta-Cyclodextrins
D000596:Amino Acids; D003505:Cyclodextrins; D007202:Indicators and Reagents; D008567:Membranes, Artificial; D023303:Oxazolidinones; D047392:beta-Cyclodextrins; D012710:Serum Albumin, Bovine; D002482:Cellulose; C024671:cellulose triacetate; C031215:betadex