title: Quantification of L-ascorbic acid and total ascorbic acid in fruits and spinach by capillary zone electrophoresis.
abstract: A standard curve for the quantification of L-ascorbic acid (L-AA) by capillary zone electrophoresis (CZE) was established, and the quantification of ascorbic acid and total ascorbic acid in fruits (lemon, Sunkist, and pineapple) and spinach were performed using D-isoascorbic acid (D-IAA) as an internal standard. The minimum detection limits (MDLs) for L-AA and D-IAA were determined to be 1 and 2 microg/mL, respectively, at 265 nm. Dehydroascorbic acid (DHAA) in fruits and spinach was quantified in the presence of DL-homocysteine. The recoveries for L-AA in these juices were between 95 and 105%.
authors.affiliation: Graduate Institute of Food Science and Technology, National Taiwan University, Taipei.
authors.forename: T
authors.initials: T
authors.lastname: Liao
authors.forename: C M
authors.initials: CM
authors.lastname: Jiang
authors.forename: M C
authors.initials: MC
authors.lastname: Wu
authors.forename: J Y
authors.initials: JY
authors.lastname: Hwang
authors.forename: H M
authors.initials: HM
authors.lastname: Chang
mesh_terms: D001205:Ascorbic Acid / Q000032:analysis*; D002957:Citrus / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D005638:Fruit / Q000737:chemistry*; D018724:Spinacia oleracea / Q000737:chemistry*
chemical_list: C005056:isoascorbic acid; D001205:Ascorbic Acid