title: Different localization of SeqA-bound nascent DNA clusters and MukF-MukE-MukB complex in Escherichia coli cells.
abstract: MukF, MukE and MukB proteins form a complex that may participate in the organization of folded sister chromosomes in Escherichia coli. We have found that a MukB-GFPuv4 fusion protein is observed as discrete fluorescent foci, which are localized within cellular spaces occupied by nucleoids, but not at the constriction site of cell division in living cells. In contrast, MukB-GFPuv4 is distributed throughout the whole cell when either MukF or MukE is absent. Statistical analysis revealed that most newborn cells have two foci of mukB-gfpUV4 at one-quarter and three-quarter positions in the cell length and one focus of SeqA-bound nascent DNA at or near the middle of the cell. Subsequently, the single SeqA focus divides into two foci, and then these migrate to the one-quarter and three-quarter positions. Before cell division, most long cells have two SeqA foci and four MukB-GFPuv4 foci. In early stationary phase, SeqA foci disappear, but one or two foci of MukB-GFPuv4 remain. We discuss the reorganization and proper arrangement of folded sister chromosome in the cell quarter positions, which are performed after release from the long-time cohesion of sister chromosomes.
authors.affiliation: Department of Molecular Cell Biology, Institute of Molecular Embryology and Genetics, Kumamoto University, Kuhonji 4-24-1, Kumamoto 862-0976, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Ohsumi
authors.forename: M
authors.initials: M
authors.lastname: Yamazoe
authors.forename: S
authors.initials: S
authors.lastname: Hiraga
mesh_terms: D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D002455:Cell Division; D002868:Chromosomal Proteins, Non-Histone; D002876:Chromosomes, Bacterial; D003470:Culture Media; D004269:DNA, Bacterial / Q000378:metabolism*; D004268:DNA-Binding Proteins; D003627:Data Interpretation, Statistical; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D029968:Escherichia coli Proteins; D019084:Fluorescent Antibody Technique, Indirect; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008856:Microscopy, Fluorescence; D009154:Mutation; D009876:Operon; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012097:Repressor Proteins; D014157:Transcription Factors
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D002868:Chromosomal Proteins, Non-Histone; D003470:Culture Media; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D008164:Luminescent Proteins; C067035:MukB protein, E coli; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins; C089746:SeqA protein, E coli; D014157:Transcription Factors; C087115:mukE protein, E coli; C087116:mukF protein, E coli; D049452:Green Fluorescent Proteins