Imaging infection with LeuTech.
LeuTech is a 99Tcm labelled, anti-CD15, IgM, murine monoclonal antibody shown to have high affinity (Kd = 10(-11) M) for CD15 receptors (5.1 x 10(5)/cell) expressed on human neutrophils. LeuTech was injected directly, intravenously, and its efficacy in imaging infection in 46 consecutive patients was determined. Human anti-mouse antibody (HAMA) response was examined in 30 normal volunteers using a standard LeuTech dose reconstituted with decayed 99Tcm solution. There were 38 true positive, six true negative, and two false negative scans. Of the 38 positive images, 33 (92%) were positive within 10 min after injection of LeuTech. LeuTech accuracy in this group of patients was 96%, sensitivity 95%, specificity 100%, positive predictive value (PPV) 100%, and negative predictive value (NPV) 75%. No elevation of the HAMA titre was observed in any of the 30 normal volunteers and no adverse reaction was noted in any patient. LeuTech is a highly promising agent for rapid imaging of infectious foci.
Thakur
M L
ML
Department of Radiology, Thomas Jefferson University, Philadelphia, PA 19107, USA. Mathew.Thakur@mail.tju.edu
Marcus
C S
CS
Kipper
S L
SL
Ahdoot
R
R
Diggles
L
L
Pham
H L
HL
Minami
C
C
Rhodes
Patel
M C
MC
Smith
T
T
D000328:Adult; D000818:Animals; D000911:Antibodies, Monoclonal; D001064:Appendicitis / Q000000981:diagnostic imaging; D001847:Bone Diseases / Q000000981:diagnostic imaging; D005188:False Negative Reactions; D005260:Female; D006801:Humans; D007239:Infections / Q000000981:diagnostic imaging*; D016256:Lewis X Antigen / Q000276:immunology; D008171:Lung Diseases / Q000000981:diagnostic imaging; D008297:Male; D051379:Mice; D008875:Middle Aged; D011237:Predictive Value of Tests; D019275:Radiopharmaceuticals; D012016:Reference Values; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D015899:Tomography, Emission-Computed, Single-Photon
D000911:Antibodies, Monoclonal; D016256:Lewis X Antigen; D019275:Radiopharmaceuticals; C414128:techneticum TC-99M fanolesomab