Preferential expression of reg I beta gene in human adult pancreas.
In human pancreas two genes, reg I alpha and reg I beta, have been characterized but only the reg I alpha protein has been isolated from human pancreatic secretion. To examine their respective physiological roles in fetal and adult pancreas we have compared the patterns of gene expression using a specific RT-PCR method. No progressive evolution in the two mRNAs levels was observed during fetal development (16--41 weeks). A discoordinate expression of the two genes was found with a higher level of reg I alpha mRNA in fetus and a higher level of regI beta in adult. In addition, if reg I alpha mRNA level was correlated with the expression of genes encoding exocrine proteins in adults, reg I beta mRNA level presented no correlation with any ductular, endocrine, or exocrine gene expression. In human pancreatic cell lines we showed the only expression of reg I beta gene and protein. All these data suggest that the two reg genes and proteins could play different roles in the pancreas.
Sanchez
D
D
Groupe de Recherche sur les Glandes Exocrines, Faculté de Médecine, 27, boulevard Jean Moulin, 13385 Marseille Cedex 5, France.
Figarella
C
C
Marchand-Pinatel
S
S
Bruneau
N
N
Guy-Crotte
O
O
D000368:Aged; D015415:Biomarkers / Q000032:analysis; D002135:Calcium-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics; D002460:Cell Line; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D017403:In Situ Hybridization; D051847:Lithostathine; D008875:Middle Aged; D009419:Nerve Tissue Proteins; D010179:Pancreas / Q000196:embryology / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis; D014158:Transcription, Genetic
D015415:Biomarkers; D002135:Calcium-Binding Proteins; D051847:Lithostathine; D009419:Nerve Tissue Proteins; C497617:REG1A protein, human; C497618:REG1B protein, human; D012333:RNA, Messenger