[Determination of opiates in urine: interpretation of comparison between the EIA and FPIA and confirmation of data with GC/MS].
BACKGROUND
The aim of this study was to evaluate some urine samples and to analyse them using two immunochemical screening methods.


METHODS
A more reliable method has been used to obtain a critical analysis of the validity of the first analysis methods used. A total of 147 urine samples were examined using both the FPIA technique (Adx, Abbott) and the EIA technique (Random 120, Bracco); confirmation was obtained using GC/MS.


RESULTS
When the threshold was altered from 200 to 300 ng/ml the EIA technique is more severely affected by changes in reference values compared to FPIA. In different values between two techniques, confirmation with GC/MS was possible only 5 cases.


CONCLUSIONS
The study of data processing with immunochemical tests and after confirmation showed that the results with GC/MS are closer to Adx. The FPIA results are in agreement with the GC/MS technique both at 200 and 300 ng/ml with a percentage of 80.0%. The EIA technique has a different result, at 200 ng/ml it is in agreement for 40.0%, but at 300 ng/ml there is only 20.0%.
Giusiani
M
M
Dipartimento di Biomedicina, Sperimentale, Infettiva e Pubblica, Università degli Studi, Pisa, Italy.
Viviani
P
P
D016231:Fluorescence Polarization Immunoassay; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007124:Immunoenzyme Techniques; D009294:Narcotics / Q000652:urine*
D009294:Narcotics