Matrix metalloproteinase-2 in synovial lavage fluid of patients with disorders of the temporomandibular joint.
We examined the matrix metalloproteinase-2 (MMP-2) activity in synovial lavage fluid of patients with disorders of the temporomandibular joint (TMJ) and explored the possible correlationship between MMP-2 activity and radiological changes. We studied 86 patients and 10 healthy volunteers. An arthrogram and a double contrast arthrotomogram were taken to evaluate intra-articular morphological changes. The patients were divided into three groups: no abnormality (n = 36), internal derangement (n = 39), and osteoarthritis (n = 11). Samples of synovial fluid were studied by gelatin zymography, and we sought a correlation between the band detected and radiological findings. ProMMP-2 was detected in all samples and active MMP-2 was detected in 9/36 with no abnormality, 14/39 with internal derangement and 5/11 with osteoarthritis. No active form of MMP-2 was detected in the control group. The incidence of active MMP-2 was high in the internal derangement group and highest in the osteoarthritis group, which suggests that active MMP-2 plays an important part in the development of conditions of the TMJ.
Mizui
T
T
Department of Oral and Maxillofacial Surgery, Gifu Prefectural Gifu Hospital, Noishiki, Gifu, Japan.
Ishimaru
J
J
Miyamoto
K
K
Kurita
K
K
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D004204:Joint Dislocations / Q000201:enzymology; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D008875:Middle Aged; D010003:Osteoarthritis / Q000201:enzymology; D013582:Synovial Fluid / Q000201:enzymology*; D013705:Temporomandibular Joint Disorders / Q000000981:diagnostic imaging / Q000201:enzymology*; D014057:Tomography, X-Ray Computed
D020778:Matrix Metalloproteinase 2