title: Oxidative stress could precede endothelial dysfunction and insulin resistance in Indian Mauritians with impaired glucose metabolism.
abstract: OBJECTIVE
  To measure oxidative stress, endothelial dysfunction and insulin resistance in Indian Mauritians at different stages of development of Type II (non-insulin-dependent) diabetes mellitus.
  
  
  METHODS
  Plasma total 8-epi-PGF2alpha, an indicator of oxidative stress, was determined in age-matched subjects with normal glucose metabolism (n = 39), impaired glucose tolerance (n = 14), newly diagnosed diabetes (n = 8) and established diabetes (n = 14). Plasma glucose and insulin were measured at baseline and 2 h following an oral glucose tolerance test. Endothelial function was assessed by non-invasive digital pulse wave photoplethysmography.
  
  
  RESULTS
  Plasma 8-epi-PGF2alpha increased in subjects with impaired glucose tolerance (p < 0.05) compared with control subjects, and was even higher in newly diagnosed diabetic patients (p < 0.01) and established (p < 0.01) diabetic patients. A tendency towards reduced endothelial function in subjects with impaired glucose tolerance became significant in patients with newly diagnosed and established diabetes (p < 0.01), and was correlated with 8-epi-PGF2alpha (r = 0.36, p < 0.01). Insulin resistance (homeostasis model assessment) did not change in subjects with impaired glucose tolerance compared with control subjects, but increased in newly diagnosed (p < 0.01) and established (p < 0.001) diabetic subjects. The 8-epi-PGF2alpha was correlated with fasting glucose (r = 0.50, p < 0.001), triglycerides (r = 0.40, p < 0.001) and insulin resistance (r = 0.35, p < 0.001).
  
  
  CONCLUSIONS
  Oxidant stress is an early event in the evolution of Type II diabetes and could precede the development of endothelial dysfunction and insulin resistance.
authors: Department of Experimental Therapeutics, St Bartholomew and the Royal London School of Medicine and Dentistry, Queen Mary, UK.
authors: N K
authors: NK
authors: Gopaul
authors: M D
authors: MD
authors: Manraj
authors: A
authors: A
authors: Hébé
authors: S
authors: S
authors: Lee Kwai Yan
authors: A
authors: A
authors: Johnston
authors: M J
authors: MJ
authors: Carrier
authors: E E
authors: EE
authors: Anggård
mesh_terms: D001786:Blood Glucose / Q000032:analysis; D003924:Diabetes Mellitus, Type 2 / Q000503:physiopathology*; D015237:Dinoprost / Q000031:analogs & derivatives*; D004730:Endothelium, Vascular / Q000503:physiopathology*; D028441:F2-Isoprostanes / Q000097:blood; D005215:Fasting / Q000097:blood; D005260:Female; D005947:Glucose / Q000378:metabolism*; D018149:Glucose Intolerance; D006801:Humans; D007194:India / Q000208:ethnology; D007333:Insulin Resistance; D008297:Male; D008436:Mauritius; D008875:Middle Aged; D018384:Oxidative Stress; D012016:Reference Values; D014280:Triglycerides / Q000097:blood
chemical_list: D001786:Blood Glucose; D028441:F2-Isoprostanes; D014280:Triglycerides; C075750:8-epi-prostaglandin F2alpha; D015237:Dinoprost; D005947:Glucose