title: Detection of abundantly transcribed genes and gene translocation in human immunodeficiency virus-associated non-Hodgkin's lymphoma.
abstract: Several novel, differentially transcribed genes were identified in one centroblastic and one immunoblastic HIV-associated B-cell non-Hodgkin's lymphoma (B-NHL) by subtractive cloning. In both lymphomas, we detected an upregulated transcription of several mitochondrial genes. In the centroblastic B-NHL, we found a high level transcription of nuclear genes including the interferon-inducible gene (INF-ind), the immunoglobulin light chain gene (IgL), the set oncogene, and several unknown genes. The data obtained on upregulated expression of the genes in human B-NHL of HIV-infected patients considerably overlap with those obtained earlier for the B-NHL of simian immunodeficiency virus-infected monkeys. In the centroblastic lymphoma, one transcript revealed a fusion of the 3'-untranslated region of the set gene and the C-terminal region of the IgL gene. This chimeric sequence was confirmed by a site-directed polymerase chain reaction performed with total cDNA and genomic DNA. The expected amplification product was obtained in both cases pointing to a genomic rearrangement. The IgL-set fusion sequence was not found in cDNA preparations and genomic DNA of the immunoblastic HIV-associated B-NHL. Further studies are necessary to determine whether these genes contribute to lymphoma development or can be used as therapeutic targets.
authors: Department of Viral and Cellular Molecular Genetics, Institute of Molecular Genetics, Moscow 123182, Russia. tarantul@img.ras.ru
authors: V
authors: V
authors: Tarantul
authors: A
authors: A
authors: Nikolaev
authors: H
authors: H
authors: Hannig
authors: B
authors: B
authors: Kalmyrzaev
authors: I
authors: I
authors: Muchoyan
authors: V
authors: V
authors: Maximov
authors: V
authors: V
authors: Nenasheva
authors: V
authors: V
authors: Dubovaya
authors: G
authors: G
authors: Hunsmann
authors: W
authors: W
authors: Bodemer
mesh_terms: D020413:3' Untranslated Regions; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism; D016208:Databases, Factual; D004305:Dose-Response Relationship, Drug; D006801:Humans; D015151:Immunoblotting; D007136:Immunoglobulins / Q000378:metabolism; D008223:Lymphoma / Q000378:metabolism; D016483:Lymphoma, AIDS-Related / Q000378:metabolism*; D008228:Lymphoma, Non-Hodgkin / Q000821:virology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism*; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic; D015854:Up-Regulation
chemical_list: D020413:3' Untranslated Regions; D018076:DNA, Complementary; D007136:Immunoglobulins; D012333:RNA, Messenger