The first bovine beta 1,4-galactosyltransferase reaction with an acyclic acceptor substrate, 3-acetamido-1,2-propanediol, to yield a 3-O-beta-D-galactopyranosyl-sn-glycerol skeleton.
[figure: see text] Reactivity of bovine beta 1,4-galactosyltransferase was examined for a series of acyclic acceptor substrates both in the presence and the absence of alpha-lactalbumin (alpha-La). It was found that this enzyme could utilize (R)-3-acetamido-1,2-propanediol (1) as an acceptor substrate regardless of the cofactor protein. The product was determined to be 1-O-beta-D-galactopyranosyl-(R)-3-acetamido-1,2-propanediol (2). Glycerol without the acetamido group was inactive, indicating that this functional group plays a key role in the enzyme reaction.
Nishida
Y
Y
Department of Molecular Design and Engineering, Graduate School of Engineering, Nagoya University, Chikusa-ku, Nagoya 464-8603, Japan. nishida@mol.nagoya-u.ac.jp
Tamakoshi
H
H
Kobayashi
K
K
Thiem
J
J
D000818:Animals; D002384:Catalysis; D002417:Cattle; D005697:Galactosides / Q000737:chemistry; D007768:Lactalbumin / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D009005:Monosaccharides / Q000378:metabolism; D009237:N-Acetyllactosamine Synthase / Q000378:metabolism*; D011409:Propylene Glycols / Q000737:chemistry / Q000378:metabolism*; D013237:Stereoisomerism; D013329:Structure-Activity Relationship; D013379:Substrate Specificity
C430343:1-O-galactopyranosyl-3-acetamido-1,2-propanediol; C430342:3-acetamido-1,2-propanediol; D005697:Galactosides; D009005:Monosaccharides; D011409:Propylene Glycols; D007768:Lactalbumin; D009237:N-Acetyllactosamine Synthase