[Expression of delta 6-fatty acid desaturase gene from Mortierella alpina in Saccharomyces cerevisiae].
delta 6-fatty acid desaturase is the rate-limiting enzyme of the desaturation of linoleic acid in the production of an essential fatty acid, gamma-linolenic acid. The 1.4 kb fragment in plasmid pTMACL6 encoding delta 6-fatty acid desaturase from Mortieralla alpina ATCC16266 was subcloned into the yeast-E. coli shuttle vector pYES2.0, thus an expression recombinant plasmid pYMAD6 containing target gene was constructed and obtained in the SC-Ura media. The pYMAD6 was introduced into defective mutant INCSc1 of Saccharomyces cerevisiae by LiAc method. When linoleic acid was provided as an exogenous substrate to the yeast cultures expressing delta 6-fatty acid desaturase activity under appropriate media and temperature condition, the level of gamma-linolenic acid reached 31.6% of the total yeast fatty acids by GC-MS detecting, which is the highest report of delta 6-fatty-acid desaturase gene in S. cerevisiae.
Liu
L
L
Department of Microbiology, NanKai University, Tianjin 300071, China.
Li
M C
MC
Hu
G W
GW
Ge
J
J
Zhang
L
L
Cheng
Z H
ZH
Xing
L J
LJ
D044943:Fatty Acid Desaturases / Q000235:genetics*; D020094:Mortierella / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D017962:alpha-Linolenic Acid / Q000096:biosynthesis
D017962:alpha-Linolenic Acid; D044943:Fatty Acid Desaturases