title: Evaluation of a rapid western immunoblotting procedure for the diagnosis of bovine spongiform encephalopathy (BSE) in the UK.
abstract: Bovine brain tissue samples from 625 UK cattle, clinically suspected as bovine spongiform encephalopathy (BSE) cases, were used in a blind analysis to assess a rapid Western immunoblotting technique (Prionics Check; Prionics AG, Zurich), which detects bovine disease-specific protease-resistant prion protein (PrP(Sc)). By means of statutory histopathological examination, 599 of the 625 cattle were confirmed as BSE cases by the demonstration of spongiform encephalopathy, the remaining 26 being classified as negative. Duplicate samples from the same animals were also examined by electron microscopy for the presence of abnormal brain fibrils (scrapie-associated fibrils; SAFs). The Prionics technique showed a high sensitivity, particularly when compared with the fibril detection test; the detection rates were 99.3% and 92.0% respectively, with histopathology being used as the "gold standard". The false negative results by the Prionics test were possibly related to the sampling procedure. Analysis of 50 BSE-positive samples revealed similar glycoprofiles, the majority of PrP(Sc)isoforms being di-glycosylated protein. The Prionics test also detected PrP(Sc)in the four brain samples from the 26 histopathologically negative animals, apparently reducing the specificity of the test to 84.6%; however, confirmatory positive results in these samples were obtained by demonstrating SAF or by immunohistochemical examination, or both. It was concluded that the Prionics test detected PrP(Sc)in a small percentage (0.64%) of clinically suspected BSE cases showing no spongiform change. Since January 2000, the Prionics Western blot test has been introduced as one of the statutory tests for the diagnosis of clinically suspected BSE and scrapie cases in the UK.
authors: Veterinary Laboratories Agency, Weybridge, Surrey, KT15 3NB, UK.
authors: W A
authors: WA
authors: Cooley
authors: J K
authors: JK
authors: Clark
authors: S J
authors: SJ
authors: Ryder
authors: L A
authors: LA
authors: Davis
authors: S S
authors: SS
authors: Farrelly
authors: M J
authors: MJ
authors: Stack
mesh_terms: D000818:Animals; D015153:Blotting, Western / Q000379:methods / Q000662:veterinary*; D001923:Brain Chemistry; D002417:Cattle; D016643:Encephalopathy, Bovine Spongiform / Q000175:diagnosis* / Q000453:epidemiology / Q000821:virology; D007150:Immunohistochemistry / Q000662:veterinary; D008854:Microscopy, Electron / Q000662:veterinary; D018622:PrP 27-30 Protein / Q000032:analysis* / Q000648:ultrastructure; D012680:Sensitivity and Specificity; D016037:Single-Blind Method; D006113:United Kingdom / Q000453:epidemiology
chemical_list: D018622:PrP 27-30 Protein