Migration and multipotentiality of PSA-NCAM+ neural precursors transplanted in the developing brain.
By optimizing the previously described strategy for obtention of spheres enriched in PSA-NCAM+ precursors, we prepared PSA-NCAM-immunoselected cell populations from cerebral hemispheres of neonatal MBP-LacZ transgenic mice. These cells expressed Nestin, exhibited clonal expansion potential and formed spheres, which were initially enriched in PSA-NCAM+ cells but became enriched in GD3+ oligodendrocyte progenitors after 1 week in B104 contionned medium. One month after their periventricular transplantation into the brain of wild-type and/or shiverer newborn mice, cells from PSA-NCAM+ spheres exhibited a higher rostral migration potential than cells from GD3+ spheres, and clearly contributed to myelination in the olfactory bulb. In shiverer hosts, both sphere populations generated oligodendrocytes with similar myelination potential. In addition PSA-NCAM+ sphere cells generated GFAP+ astrocytes and NeuN+ neurons, depending on their site of insertion. These results evidence the high plasticity of newborn PSA-NCAM+ neural precursors and suggest that they are promising tools for cell therapy of CNS diseases, including myelin disorders.
Vitry
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S
Institut National de la Santé et de la Recherche Médicale U546, CHU Pitié-Salpêtrière, Paris cedex 13, 75634, France.
Avellana-Adalid
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V
Lachapelle
F
F
Baron-Van Evercooren
A
A
D000818:Animals; D000943:Antigens, Differentiation / Q000378:metabolism; D001253:Astrocytes / Q000166:cytology / Q000378:metabolism; D001921:Brain / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism; D016380:Brain Tissue Transplantation / Q000379:methods; D002449:Cell Aggregation / Q000235:genetics; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation / Q000502:physiology*; D019070:Cell Lineage / Q000502:physiology*; D002465:Cell Movement / Q000502:physiology*; D002478:Cells, Cultured / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D002493:Central Nervous System Diseases / Q000601:surgery; D002999:Clone Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D003470:Culture Media / Q000494:pharmacology; D005732:Gangliosides / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D006085:Graft Survival / Q000502:physiology; D007381:Intermediate Filament Proteins / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D009419:Nerve Tissue Proteins; D064231:Nestin; D039842:Neural Cell Adhesion Molecule L1; D019006:Neural Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism*; D009474:Neurons / Q000166:cytology / Q000378:metabolism / Q000637:transplantation*; D009836:Oligodendroglia / Q000166:cytology / Q000378:metabolism; D012794:Sialic Acids / Q000235:genetics / Q000378:metabolism*; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism; D013788:Thalamus / Q000166:cytology / Q000254:growth & development / Q000601:surgery
D000943:Antigens, Differentiation; D003470:Culture Media; D005732:Gangliosides; D005904:Glial Fibrillary Acidic Protein; D007381:Intermediate Filament Proteins; D009419:Nerve Tissue Proteins; C577485:Nes protein, mouse; D064231:Nestin; D039842:Neural Cell Adhesion Molecule L1; D019006:Neural Cell Adhesion Molecules; D012794:Sialic Acids; C036505:oligodendrocyte O antigen, mouse; C103469:polysialyl neural cell adhesion molecule; C026226:ganglioside, GD3