[Construction of luciferase reporter plasmid which is under the control of dioxin-responsive enhancers].
To improve the bio-detection of dioxin-type chemicals, we constructed a plasmid containing reporter under the control of dioxin-responsive enhancer. After connected the enhancer from the plasmid pHAV with the MMTV promoter of the plasmid pCatM, the connected segment was linked with the luciferase vector. Then the recombinant plasmid was transfected to the HepG2 human hepatoma cell line, and the luciferase activity was induced by 2,3,7,8-TCDD. The result suggested that expression of the recombinant luciferase was controlled by the dioxin-responsive enhancer, and there were a linear relationship between the activity of luciferase and certain range of 2,3,7,8-TCDD concentration. It is promising that the transfected cell line could be used for rapid screen and semi quantitation of dioxin-type chemicals, and a standard and routine method for the detection of dioxin-type chemicals by this recombinant plasmid is being under way.
Zhang
Z R
ZR
Institute of Environmental Monitoring, Department of Environmental Medicine, Tongji Medical University, Wuhan 430030, China. zhangzhiren@yahoo.com
Xu
S Q
SQ
Zhou
Y K
YK
Ren
S
S
Lü
B
B
D004742:Enhancer Elements, Genetic; D017930:Genes, Reporter; D008156:Luciferases / Q000235:genetics*; D010957:Plasmids; D000072317:Polychlorinated Dibenzodioxins / Q000032:analysis / Q000494:pharmacology*
D000072317:Polychlorinated Dibenzodioxins; D008156:Luciferases