[Differential binding of hyaluronic acid in 2 CD44+ sublines. Relationship with tumor infiltration].
We have established and characterized a cell line (LBL) from a spontaneous murine T lymphoma LB. Histopathological analysis has demonstrated LB primary tumor infiltration in spleen, lymph nodes, liver, thymus, bone marrow and lung. However LBL cells infiltrated all these organs except lung. Two sublines with different growth behavior were derived from LBL cell line. One of them grew in suspension as clusters (LBLc) while the other one grew as adherent monolayers (LBLa). Growth rate, response to mitogenic stimuli and apoptosis induction were different among the parental cell line and the derived sublines. CD44 was expressed constitutively in LBL and LBLa cells. In contrast LBLc cells only expressed similar levels of this molecule when stimulated with PMA. LBLa cells showed hyaluronic acid (HA) binding properties, while LBL and LBLc cells were not able to bind HA even when activated with PMA. We postulate that differences in HA binding could be related with different infiltration behaviors.
Ernst
G M
GM
Instituto de Estudios de la Inmunidad Humoral (IDEHU), Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, 1113 Buenos Aires, Argentina.
Caldas Lopes
E
E
Cabrera
P V
PV
Alvarez
E
E
Hajos
S E
SE
D000276:Adjuvants, Immunologic / Q000378:metabolism*; D000818:Animals; D002455:Cell Division; D016001:Confidence Intervals; D005434:Flow Cytometry; D018960:Hyaluronan Receptors / Q000378:metabolism*; D006820:Hyaluronic Acid / Q000378:metabolism*; D015458:Leukemia, T-Cell / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009361:Neoplasm Invasiveness; D011485:Protein Binding; D014407:Tumor Cells, Cultured / Q000378:metabolism / Q000473:pathology*
D000276:Adjuvants, Immunologic; D018960:Hyaluronan Receptors; D006820:Hyaluronic Acid