title: PEAZ-1: a new human prostate neoplastic epithelial cell line.
abstract: BACKGROUND
  The generation of prostatic cell lines provides in vitro models for experimental studies of the pathogenesis of prostate carcinoma. Therefore, we established and characterized a new human prostate epithelial cell line, PEAZ-1 (prostate epithelial Arizona-1).
  
  
  METHODS
  The PEAZ-1 cells were grown from a primary human prostate carcinoma specimen obtained from radical prostatectomy. The isolated cells were characterized by immunobiochemistry, immunohistochemistry, and tumorigenicity studies.
  
  
  RESULTS
  PEAZ-1 cells are near diploid, tumorigenic, and androgen independent for cell growth. PEAZ-1 cells express N-cadherin, alpha- and beta-catenins, and p120 at cell-cell contacts, cytoplasmic laminin 5, vinculin, paxillin, and phosphotyrosine at focal adhesions, vimentin, and cytokeratins 8 and 18. They do not express plakoglobin, E-cadherin, and PSA, and do not form desmosomes and hemidesomomes. PEAZ-1 respond to ocadaic acid, a pro-apoptotic agent, by expression of p53.
  
  
  CONCLUSIONS
  PEAZ-1 cells is a human prostate cancer cell line that has a number of mesenchymal characteristics.
authors: Department of Pathology, University of Arizona Health Sciences Center, Tucson, Arizona 85724-5043, USA. schmelz@u.arizona.edu
authors: M
authors: M
authors: Schmelz
authors: A E
authors: AE
authors: Cress
authors: J
authors: J
authors: Barrera
authors: K M
authors: KM
authors: McDaniel
authors: T L
authors: TL
authors: Davis
authors: L
authors: L
authors: Fuchs
authors: B L
authors: BL
authors: Dalkin
authors: R B
authors: RB
authors: Nagle
mesh_terms: D000728:Androgens / Q000494:pharmacology; D015820:Cadherins / Q000494:pharmacology; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D016023:Integrins / Q000032:analysis; D008297:Male; D011468:Prostatectomy; D011471:Prostatic Neoplasms / Q000473:pathology*; D013048:Specimen Handling; D014407:Tumor Cells, Cultured
chemical_list: D000728:Androgens; D015820:Cadherins; D016023:Integrins