Comparison of the Reveal 20-hour method and the BAM culture method for the detection of Escherichia coli O157:H7 in selected foods and environmental swabs: collaborative study.
Four different food types along with environmental swabs were analyzed by the Reveal for E. coli O157:H7 test (Reveal) and the Bacteriological Analytical Manual (BAM) culture method for the presence of Escherichia coli O157:H7. Twenty-seven laboratories representing academia and private industry in the United States and Canada participated. Sample types were inoculated with E. coli O157:H7 at 2 different levels. Of the 1,095 samples and controls analyzed and confirmed, 459 were positive and 557 were negative by both methods. No statistical differences (p <0.05) were observed between the Reveal and BAM methods.
Bird
C B
CB
Neogen Corp, Lansing, MI 48912, USA.
Hoerner
R J
RJ
Restaino
L
L
D001628:Beverages / Q000382:microbiology; D003470:Culture Media; D004783:Environmental Microbiology / Q000592:standards; D019453:Escherichia coli O157 / Q000254:growth & development / Q000302:isolation & purification*; D005516:Food Microbiology / Q000592:standards; D005638:Fruit; D007163:Immunosorbent Techniques; D007753:Laboratories; D018545:Lactuca / Q000382:microbiology; D008828:Microbiological Techniques
D003470:Culture Media