title: Two-dimensional electrophoresis and characterization of antigens from Paracoccidioides brasiliensis.
abstract: Paracoccidioides brasiliensis is a fungal pathogen of humans. To identify antigens from P. brasiliensis we fractionated a crude preparation of proteins from the fungus and detected the IgG reactive proteins by immunoblot assays of yeast cellular extracts with sera of patients with paracoccidioidomycosis (PCM). We identified and characterized six new antigens by amino acid sequencing and homology search analyses with other proteins deposited in a database. The newly characterized antigens were highly homologous to catalase, fructose-1,6-biphosphate aldolase (aldolase), glyceraldehyde-3-phosphate dehydrogenase, malate dehydrogenase and triosephosphate isomerase from several sources. The characterized antigens presented preferential synthesis in yeast cells, the host fungus phase.
authors: Laboratório de Biologia Molecular, ICBII, UFG, Universidade Federal de Goiás, 74001-970, Go, Goiânia, Brazil.
authors: C A
authors: CA
authors: da Fonseca
authors: R S
authors: RS
authors: Jesuino
authors: M S
authors: MS
authors: Felipe
authors: D A
authors: DA
authors: Cunha
authors: W A
authors: WA
authors: Brito
authors: C M
authors: CM
authors: Soares
mesh_terms: D000595:Amino Acid Sequence; D000946:Antigens, Fungal / Q000032:analysis* / Q000737:chemistry; D015180:Electrophoresis, Gel, Two-Dimensional; D005656:Fungal Proteins / Q000032:analysis* / Q000737:chemistry; D006801:Humans; D015151:Immunoblotting; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D008969:Molecular Sequence Data; D010228:Paracoccidioides / Q000276:immunology*; D010229:Paracoccidioidomycosis / Q000276:immunology*; D017386:Sequence Homology, Amino Acid
chemical_list: D000946:Antigens, Fungal; D005656:Fungal Proteins; D007074:Immunoglobulin G