Hermes-mediated germ-line transformation of the Mediterranean fruit fly Ceratitis capitata.
We report the use of the Hermes transposable element for germ-line transformation of the Mediterranean fruit fly, Ceratitis capitata. Hermes was able to genetically transform this insect at an estimated frequency between 0.6 and 1.1%, which is comparable to the transformation frequencies obtained for this species when using other transposable elements. Hermes integrates into the medfly genome by a cut-and-paste mechanism and the sequences integrated into the genome are delimited by the terminal nucleotides of the Hermes inverted terminal repeats. Integration resulted in the generation of 8 bp target site duplications, the sequences of which conformed to the target site duplications generated by hAT element transposition in insects. The Hermes element is one additional genetic tool that can be deployed in manipulating and characterizing the medfly genome.
Michel
K
K
Department of Entomology, University of California, Riverside, CA 92521, USA.
Stamenova
A
A
Pinkerton
A C
AC
Franz
G
G
Robinson
A S
AS
Gariou-Papalexiou
A
A
Zacharopoulou
A
A
O'Brochta
D A
DA
Atkinson
P W
PW
D000818:Animals; D004251:DNA Transposable Elements; D004175:Diptera / Q000235:genetics*; D005854:Germ Cells; D014170:Transformation, Genetic
D004251:DNA Transposable Elements