A mechanism-based affinity-labeling agent for possible use in isolating N-acetylglucosaminidase.
We have prepared several mechanism-based affinity-labeling agents for possible use in isolating N-acetylglucosaminidase, in which an N-acetylglucosamine is linked to an o-monofluoro- or difluoro-methyl phenoxy glycoside with or without a cleavable disulfide group in the tether to biotin.
Ichikawa
M
M
Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, 21205, Baltimore, MD, USA.
Ichikawa
Y
Y
D000118:Acetylglucosaminidase / Q000737:chemistry*; D000345:Affinity Labels; D004591:Electrophoresis, Polyacrylamide Gel
D000345:Affinity Labels; D000118:Acetylglucosaminidase