title: Low-pressure UV inactivation and DNA repair potential of Cryptosporidium parvum oocysts.
abstract: Because Cryptosporidium parvum oocysts are very resistant to conventional water treatment processes, including chemical disinfection, we determined the kinetics and extent of their inactivation by monochromatic, low-pressure (LP), mercury vapor lamp UV radiation and their subsequent potential for DNA repair of UV damage. A UV collimated-beam apparatus was used to expose suspensions of purified C. parvum oocysts in phosphate-buffered saline, pH 7.3, at 25 degrees C to various doses of monochromatic LP UV. C. parvum infectivity reductions were rapid, approximately first order, and at a dose of 3 mJ/cm(2) (=30 J/m(2)), the reduction reached the cell culture assay detection limit of approximately 3 log(10). At UV doses of 1.2 and 3 mJ/cm(2), the log(10) reductions of C. parvum oocyst infectivity were not significantly different for control oocysts and those exposed to dark or light repair conditions for UV-induced DNA damage. These results indicate that C. parvum oocysts are very sensitive to inactivation by low doses of monochromatic LP UV radiation and that there is no phenotypic evidence of either light or dark repair of UV-induced DNA damage.
authors: Department of Environmental Sciences and Engineering, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599-7400, USA. gwyam@isis.unc.edu
authors: G A
authors: GA
authors: Shin
authors: K G
authors: KG
authors: Linden
authors: M J
authors: MJ
authors: Arrowood
authors: M D
authors: MD
authors: Sobsey
mesh_terms: D000818:Animals; D002460:Cell Line; D003457:Cryptosporidiosis / Q000469:parasitology; D016785:Cryptosporidium parvum / Q000235:genetics / Q000254:growth & development* / Q000472:pathogenicity; D004249:DNA Damage; D004260:DNA Repair; D004203:Disinfection / Q000379:methods*; D004285:Dogs; D006863:Hydrogen-Ion Concentration; D051379:Mice; D008807:Mice, Inbred BALB C; D013696:Temperature; D014466:Ultraviolet Rays; D014774:Virulence