Effects of recombinant granulocyte-colony stimulating factor administration during Mycobacterium avium infection in mice.
Granulocyte colony-stimulating factor (G-CSF) administration in vivo has been shown to improve the defence mechanisms against infection by different microbes. Here we evaluated a possible protective role of this molecule in a mouse model of mycobacterial infection. The administration of recombinant G-CSF promoted an extensive blood neutrophilia but failed to improve the course of Mycobacterium avium infection in C57Bl/6 or beige mice. G-CSF administration also failed to improve the efficacy of a triple chemotherapeutic regimen (clarithromycin + ethambutol + rifabutin). G-CSF treatment did not protect interleukin-10 gene disrupted mice infected with M. avium. Spleen cells from infected mice treated with G-CSF had a decreased priming for antigen-specific production of interferon gamma compared to control infected mice. Our data do not substantiate previous reports on the protective activity of G-CSF in antimycobacterial immunity using mouse models.
Gonçalves
A S
AS
Laboratory of Microbiology and Immunology of Infection, Institute for Molecular and Cell Biology, University of Porto, Portugal.
Appelberg
R
R
D000818:Animals; D023181:Antimicrobial Cationic Peptides; D000995:Antitubercular Agents / Q000627:therapeutic use; D001798:Blood Proteins; D017291:Clarithromycin / Q000627:therapeutic use; D004977:Ethambutol / Q000627:therapeutic use; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000627:therapeutic use*; D007371:Interferon-gamma / Q000378:metabolism; D016753:Interleukin-10 / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D009162:Mycobacterium avium; D009504:Neutrophils / Q000166:cytology; D011994:Recombinant Proteins; D017828:Rifabutin / Q000627:therapeutic use; D014376:Tuberculosis / Q000188:drug therapy*
D023181:Antimicrobial Cationic Peptides; D000995:Antitubercular Agents; D001798:Blood Proteins; D011994:Recombinant Proteins; C030509:neutrophil basic proteins; D016753:Interleukin-10; D016179:Granulocyte Colony-Stimulating Factor; D017828:Rifabutin; D007371:Interferon-gamma; D004977:Ethambutol; D017291:Clarithromycin