Bifidobacterium longum as a delivery system for gene therapy of chemically induced rat mammary tumors.
A fundamental obstacle in cancer gene therapy is the specific targeting of therapy directly to a solid tumor, and no systemic delivery system yet exists. A strain of domestic bacteria, Bifidobacterium longum, which is nonpathogenic and anaerobic, selectively localized to and proliferated in 7,12-dimethylbenz[a]anthracene-induced rat mammary tumors after systemic application. We further ascertained the tumor specificity of genetically engineered, as well as wild-type, Bifidobacterium longum. This is the first demonstration that Bifidobacterium longum can be utilized as a specific gene delivery vector for gene therapy on solid breast tumors.
Yazawa
K
K
Department of Surgery, Research Center on Aging and Adaptation, Shinshu University School of Medicine, Matsumoto, Japan.
Fujimori
M
M
Nakamura
T
T
Sasaki
T
T
Amano
J
J
Kano
Y
Y
Taniguchi
S
S
D015127:9,10-Dimethyl-1,2-benzanthracene; D000818:Animals; D001644:Bifidobacterium / Q000235:genetics*; D017931:DNA Primers; D016503:Drug Delivery Systems; D005260:Female; D005818:Genetic Engineering; D015316:Genetic Therapy; D008325:Mammary Neoplasms, Experimental / Q000139:chemically induced / Q000628:therapy*; D016133:Polymerase Chain Reaction; D051381:Rats; D017207:Rats, Sprague-Dawley
D017931:DNA Primers; D015127:9,10-Dimethyl-1,2-benzanthracene