title: Potent neutralization of primary human immunodeficiency virus clade C isolates with a synergistic combination of human monoclonal antibodies raised against clade B.
abstract: OBJECTIVE
  We investigated the ability of several human neutralizing monoclonal antibodies (mAbs), originally raised against human immunodeficiency virus (HIV) clade B isolates, to neutralize primary clade C isolates as single agents and in combination.
  
  
  METHODS
  HIV clade C isolates from five different countries were tested for susceptibility to neutralization by anti-clade B mAbs in human peripheral blood mononuclear cells. Monoclonal antibody combinations were evaluated for possible synergy.
  
  
  RESULTS
  All 20 primary HIV clade C isolates could be neutralized 97.5% to 100% by a quadruple combination of mAbs IgG1b12, 2G12, 2F5, and 4E10. These mAbs recognized conserved epitopes and were highly synergistic, resulting in strong cross-clade neutralization.
  
  
  CONCLUSIONS
  In our previous experiment, a synergistic combination of human neutralizing mAbs protected all macaque neonates against oral challenge with a simian-human immunodeficiency virus encoding HIV env. Together, our data suggest that passive immunization with currently available anti-clade B mAbs could play a role in preventing HIV clade C transmission through breastfeeding.
authors: Department of Cancer Immunology and AIDS, Dana-Farber Cancer Institute, Boston, Massachusetts 02115, USA.
authors: W
authors: W
authors: Xu
authors: B A
authors: BA
authors: Smith-Franklin
authors: P L
authors: PL
authors: Li
authors: C
authors: C
authors: Wood
authors: J
authors: J
authors: He
authors: Q
authors: Q
authors: Du
authors: G J
authors: GJ
authors: Bhat
authors: C
authors: C
authors: Kankasa
authors: H
authors: H
authors: Katinger
authors: L A
authors: LA
authors: Cavacini
authors: M R
authors: MR
authors: Posner
authors: D R
authors: DR
authors: Burton
authors: T C
authors: TC
authors: Chou
authors: R M
authors: RM
authors: Ruprecht
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D004357:Drug Synergism; D015483:HIV Antibodies / Q000276:immunology; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000145:classification* / Q000276:immunology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000821:virology; D009500:Neutralization Tests
chemical_list: D000911:Antibodies, Monoclonal; D015483:HIV Antibodies