Protease XXIV increases detection of mucin gene expression during in situ hybridization in archival tissue.
Digoxigenin-labeled riboprobes of six groups of human mucins were evaluated for sensitivity in archival tissue, using protease XXIV or proteinase K during in situ hybridization. (J Histochem Cytochem 49:923-924, 2001)
Rheinhardt
J M
JM
Department of Pathology, University of California-Davis, Davis, California, USA. jmrheinhardt@ucdavis.edu
Finkbeiner
W E
WE
D004076:Digoxigenin; D019286:Endopeptidase K; D015870:Gene Expression; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods; D009077:Mucins / Q000235:genetics / Q000378:metabolism*; D015347:RNA Probes; D020860:Subtilisin
D009077:Mucins; D015347:RNA Probes; C430466:protease XXIV; D020860:Subtilisin; D019286:Endopeptidase K; D004076:Digoxigenin