title: A novel in vitro co-culture system for the study of maternal decidual endothelial cell-trophoblast interactions in human pregnancy.
abstract: Investigation of the pathophysiology of pre-eclampsia (characterised by insufficient invasion of the intrauterine vasculature by cytotrophoblasts) has been hampered by the absence of a suitable animal model, and ethical constraints in clinical studies. We have developed a novel in vitro human cell co-culture system allowing direct assessment of cytotrophoblast invasion of a decidual endothelial cell monolayer from the abluminal side, as occurs in vivo. This model will facilitate detection, at the cellular level, of abnormal endothelial cell-trophoblast functional interactions in pre-eclampsia and other pregnancy disorders with abnormal placentation.
authors: Department of Renal Medicine, Sydney University at Royal North Shore Hospital, St Leonards, NSW, Australia.
authors: E D
authors: ED
authors: Gallery
authors: S
authors: S
authors: Campbell
authors: B
authors: B
authors: Ilkovski
authors: M J
authors: MJ
authors: Sinosich
authors: C
authors: C
authors: Jackson
mesh_terms: D002450:Cell Communication / Q000502:physiology; D002465:Cell Movement / Q000502:physiology; D002478:Cells, Cultured; D018920:Coculture Techniques / Q000379:methods; D003656:Decidua / Q000502:physiology*; D005260:Female; D006801:Humans; D011225:Pre-Eclampsia / Q000473:pathology*; D011247:Pregnancy; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014327:Trophoblasts / Q000502:physiology*; D014591:Uterine Diseases / Q000473:pathology*
chemical_list: D013755:Tetradecanoylphorbol Acetate