Expression of PR-5d and ERF genes in cultured tobacco cells and their NaCl stress-response.
The expression of pathogenesis-related protein-5d (PR-5d) and ethylene-responsive transcriptional factors (ERF1-4) for basic PR-proteins was measured in cultured tobacco cells. The PR-5d transcript markedly increased with culture, but only constant levels of transcripts of ERF1 (trans-activator) and ERF3 (repressor) were detected in cells. These different responses of PR-5d and ERF genes were also observed under NaCl stress; PR-5d transcript decreased and ERF levels were constant under NaCl stress. The effect of post-transcriptional regulation of ERF activity, especially ERF3, on the expression of basic PR genes including PR-5d is discussed.
Koyama
T
T
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Japan.
Kitajima
S
S
Sato
F
F
D002478:Cells, Cultured; D005030:Ethylenes / Q000494:pharmacology; D015870:Gene Expression; D010940:Plant Proteins / Q000235:genetics*; D010947:Plants, Toxic; D011401:Promoter Regions, Genetic; D012323:RNA Processing, Post-Transcriptional; D012965:Sodium Chloride / Q000494:pharmacology*; D014026:Nicotiana / Q000166:cytology / Q000235:genetics* / Q000502:physiology; D014157:Transcription Factors / Q000235:genetics*
D005030:Ethylenes; D010940:Plant Proteins; D014157:Transcription Factors; C053376:pathogenesis-related proteins, plant; D012965:Sodium Chloride; C036216:ethylene