Disease activated drugs: a new concept for the treatment of asthma.
Disease activated drugs (DAD) are pro-drugs of one active principle or combinations of two drugs, which have a proven efficacy for the treatment of the target disease. In opposition to pro-drugs, DAD are activated in inflamed but not normal tissues. Due to the disease specific activation, the amount of locally released drug(s) should be related directly to the severity of the inflammation. To test this concept in asthma a PDE4 inhibitor, an isoquinoline derivative, was chemically derivatized into pro-drugs or combined with corticosteroids. These new compounds were more readily cleaved into active PDE4 inhibitor, in bronchoalveolar lavage fluid (BALF) from Brown-Norway rats with lung inflammation than in BALF from rats without airway inflammation. The DAD concept (local selective release and improved therapeutic window) was validated in vivo using the inhibition of methacholine induced bronchoconstriction in guinea pigs with or without ozone induced lung inflammation. An example of DAD hydrolysis (isoquinoline-dexamethasone) was also examined in BALF from asthmatics and healthy volunteers.
Charpiot
B
B
Research, Novartis Pharma AG, CH-4002 Basle, Switzerland. brigitte.charpiot@pharma.novatis.com
Bitsch
F
F
Buchheit
K H
KH
Channez
P
P
Mazzoni
L
L
Mueller
T
T
Vachier
I
I
Naef
R
R
D000818:Animals; D018927:Anti-Asthmatic Agents / Q000494:pharmacology / Q000627:therapeutic use*; D001249:Asthma / Q000188:drug therapy* / Q000201:enzymology; D001992:Bronchoalveolar Lavage Fluid; D016084:Bronchoconstriction; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D006168:Guinea Pigs; D006801:Humans; D006868:Hydrolysis; D066298:In Vitro Techniques; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D013058:Mass Spectrometry; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D051381:Rats
D018927:Anti-Asthmatic Agents; D010727:Phosphoric Diester Hydrolases