title: Identification of an in vitro ribonucleoprotein complex between a viroid RNA and a phloem protein from cucumber plants.
abstract: We used the interaction of Hop stunt viroid (HSVd) and cucumber plants to investigate the involvement of phloem proteins in the systemic transport of RNA molecules. A ribonucleoprotein complex, stable even at high salt and temperature conditions, was detected in vitro between HSVd-RNA and the phloem exudate obtained from sectioned internodes from cucumber plants. The phloem protein 2 was recovered from this ribonucleoprotein complex and its RNA-binding properties as demonstrated by gel retardation analysis. The involvement of this protein in the movement of RNAs in cucumber is discussed.
authors: Instituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia-CSIC, Spain.
authors: G
authors: G
authors: Gómez
authors: V
authors: V
authors: Pallás
mesh_terms: D018553:Cucumis sativus / Q000378:metabolism* / Q000821:virology*; D046911:Macromolecular Substances; D010935:Plant Diseases / Q000821:virology; D037121:Plant Lectins; D010940:Plant Proteins / Q000302:isolation & purification / Q000378:metabolism*; D012367:RNA, Viral / Q000302:isolation & purification / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism; D012261:Ribonucleoproteins / Q000302:isolation & purification / Q000378:metabolism*; D014772:Viroids / Q000378:metabolism*
chemical_list: D046911:Macromolecular Substances; D037121:Plant Lectins; D010940:Plant Proteins; D012367:RNA, Viral; D016601:RNA-Binding Proteins; D012261:Ribonucleoproteins; C091028:phloem lectin