title: Molecular cloning and characterization of a novel peptidylprolyl isomerase (cyclophilin)-like gene (PPIL3) from human fetal brain.
abstract: During the large-scale sequencing analysis of a human fetal brain cDNA library, we isolated two cDNA clones encoding two novel proteins, which show 52% and 72% identity to the cyclophilin isoform 10 of C. elegans, respectively. Sequence analysis revealed these two cDNA clones are two different splicing variants of a novel cyclophilin-like gene (PPIL3). The PPIL3 gene was identified on a completely sequenced BAC (GenBank accession AC005037) from chromosome 2q33 between STS markers stSG2762 (proximal) and SHGC-3074 (distal), oriented toward the telomere. The PPIL3 gene consisted of eight exons spanning more than 18 kb of genomic DNA. RT-PCR analysis indicated that PPIL3 was ubiquitously expressed in adult human tissues.
authors: State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai, People's Republic of China.
authors: Z
authors: Z
authors: Zhou
authors: K
authors: K
authors: Ying
authors: J
authors: J
authors: Dai
authors: R
authors: R
authors: Tang
authors: W
authors: W
authors: Wang
authors: Y
authors: Y
authors: Huang
authors: W
authors: W
authors: Zhao
authors: Y
authors: Y
authors: Xie
authors: Y
authors: Y
authors: Mao
mesh_terms: D020121:5' Untranslated Regions / Q000235:genetics; D017398:Alternative Splicing / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D001921:Brain / Q000196:embryology / Q000201:enzymology / Q000378:metabolism; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics; D002889:Chromosomes, Human, Pair 2 / Q000235:genetics*; D003001:Cloning, Molecular; D019295:Computational Biology; D021983:Cyclophilins / Q000737:chemistry / Q000235:genetics*; D005091:Exons / Q000235:genetics*; D020869:Gene Expression Profiling; D006801:Humans; D007438:Introns / Q000235:genetics*; D008969:Molecular Sequence Data; D020161:Physical Chromosome Mapping; D022821:RNA Splice Sites / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D016415:Sequence Alignment; D017421:Sequence Analysis; D016324:Sequence Tagged Sites
chemical_list: D020121:5' Untranslated Regions; D022821:RNA Splice Sites; D012333:RNA, Messenger; D021983:Cyclophilins; C431697:Ppil3 protein, rat