Morphine tolerance in mice is independent of polymorphisms in opioid receptor sequences.
Pharmacogenomics links individual drug response variation to genetic differences, such as single nucleotide polymorphisms (SNPs). In particular, pharmacogenomics will allow clinicians to use genetic diagnostics to predict the response of a patient to a drug. We investigated whether SNPs in opioid receptors correlated with the development of morphine tolerance in mouse strains that showed either high or low tolerance to morphine. Sequencing identified five silent SNPs in the delta opioid receptor that varied from the published sequence in some strains, but which were found in both high and low tolerance strains. The mu and kappa opioid receptor sequences had no SNPs. Taken together, these data definitively demonstrate that morphine tolerance development in mice is independent of opioid receptor sequence.
Gant
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Geraldine Brush Cancer Research Institute, California Pacific Medical Center Research Institute, San Francisco, CA 94115, USA.
Riba
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Lee
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D000818:Animals; D001483:Base Sequence / Q000502:physiology; D002490:Central Nervous System / Q000187:drug effects* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004361:Drug Tolerance / Q000235:genetics*; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009020:Morphine / Q000494:pharmacology; D009021:Morphine Dependence / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology; D009294:Narcotics / Q000494:pharmacology; D020641:Polymorphism, Single Nucleotide / Q000502:physiology*; D011957:Receptors, Opioid / Q000235:genetics* / Q000378:metabolism
D009294:Narcotics; D011957:Receptors, Opioid; D009020:Morphine