Recombinant hemagglutinin protein of rinderpest virus expressed in insect cells induces humoral and cell mediated immune responses in cattle.
Rinderpest virus causes a highly contagious and often fatal disease in domestic and wild ruminants. The surface glycoproteins, hemagglutinin (H) and fusion (F) proteins of this enveloped virus are known to confer protective immunity in cattle. We have reported the generation of a recombinant baculovirus expressing H protein and studied its protective properties in cattle. In this report, we demonstrate that the recombinant baculovirus encoded H protein expressed in insect cells gets incorporated into extracellular baculovirus. Single administration of low doses of purified recombinant extracellular virus with or without adjuvant induces virus neutralizing antibody responses and bovine leukocyte antigen (BoLA) class II restricted helper T cell responses in cattle.
Sinnathamby
G
G
Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore 560 012, India.
Naik
S
S
Renukaradhya
G J
GJ
Rajasekhar
M
M
Nayak
R
R
Shaila
M S
MS
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D016367:Baculoviridae / Q000235:genetics; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology / Q000517:prevention & control; D002460:Cell Line; D006023:Glycoproteins / Q000235:genetics / Q000276:immunology*; D006389:Hemagglutinins, Viral; D000949:Histocompatibility Antigens Class II / Q000378:metabolism; D007111:Immunity, Cellular; D008213:Lymphocyte Activation; D009500:Neutralization Tests; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D012301:Rinderpest / Q000276:immunology / Q000517:prevention & control; D012302:Rinderpest virus / Q000235:genetics / Q000276:immunology*; D018411:Spodoptera; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology; D014614:Vaccines, Synthetic / Q000235:genetics / Q000276:immunology / Q000494:pharmacology; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology*; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology / Q000494:pharmacology
D000914:Antibodies, Viral; D006023:Glycoproteins; D006389:Hemagglutinins, Viral; D000949:Histocompatibility Antigens Class II; D011994:Recombinant Proteins; D014614:Vaccines, Synthetic; D014764:Viral Proteins; D014765:Viral Vaccines; C079749:hemagglutinin protein, Rinderpest virus