title: Voltage-clamp recordings of postsynaptic currents in substantia gelatinosa neurons in vitro and its applications to assess synaptic transmission.
abstract: We describe here procedures for recording postsynaptic currents in substantia gelatinosa neurons on a transverse spinal cord slice preparation with an attached dorsal root. At the holding potential of -70 mV, glutamatergic spontaneous excitatory postsynaptic currents (EPSCs) and dorsal root (A delta and/or C fiber) stimulation-evoked EPSCs could be observed. Whereas at the holding potential of 0 mV, spontaneous inhibitory postsynaptic currents (IPSCs) and dorsal root A delta fiber stimulation-evoked IPSCs could be encountered. The methods make it possible to evaluate synaptic transmission by analysing the postsynaptic currents on dorsal root attached spinal cord slice.
authors: Department of Anatomy and K. K. Leung Brain Research Centre, The Fourth Military Medical University, Xi'an 710032, PR China. deptanat@fmmu.edu.cn
authors: K
authors: K
authors: Yang
authors: Y
authors: Y
authors: Li
authors: E
authors: E
authors: Kumamoto
authors: H
authors: H
authors: Furue
authors: M
authors: M
authors: Yoshimura
mesh_terms: D000818:Animals; D004594:Electrophysiology; D005071:Evoked Potentials / Q000502:physiology; D019706:Excitatory Postsynaptic Potentials / Q000502:physiology*; D066298:In Vitro Techniques; D009474:Neurons / Q000502:physiology*; D018408:Patch-Clamp Techniques / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000166:cytology; D013376:Substantia Gelatinosa / Q000166:cytology / Q000502:physiology*; D009435:Synaptic Transmission / Q000502:physiology*