title: Time-dependent expression and processing of a hypothetical protein of possible importance for regulation of the Chlamydia pneumoniae developmental cycle.
abstract: Chlamydia pneumoniae is an obligate intracellular human pathogen infecting epithelial cells of the upper respiratory tract. It is a Gram-negative bacteria and has a unique biphasic developmental cycle. In this study, we use two-dimensional gel electrophoresis in combination with radioactive labeling to investigate time-dependent expression and processing of C. pneumoniae proteins. We report on (i) the identification of a hypothetical protein which is expressed late in the developmental cycle and subsequently processed; we speculate that this protein may be of importance for the developmental cycle of Chlamydia; (ii) the identification of the major outer membrane protein in three different variants, which may all be present in vivo.
authors: Department of Medical Microbiology and Immunology, University of Aarhus, Denmark. vandahl@medmicro.au.dk
authors: B B
authors: BB
authors: Vandahl
authors: K
authors: K
authors: Gevaert
authors: H
authors: H
authors: Demol
authors: B
authors: B
authors: Hoorelbeke
authors: A
authors: A
authors: Holm
authors: J
authors: J
authors: Vandekerckhove
authors: G
authors: G
authors: Christiansen
authors: S
authors: S
authors: Birkelund
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D016993:Chlamydophila pneumoniae / Q000502:physiology*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
chemical_list: D001426:Bacterial Proteins