Ferrocene-avidin conjugates for bioelectrochemical applications.
Coupling of ferrocene moieties to avidin via a flexible spacer molecule yields a conjugate which combines the unique biotin-binding properties of avidin with the reversible redox characteristics of ferrocenes. Synthesis of the conjugate has been optimised and the conjugates were characterised bio- and electrochemically. Covalent immobilisation of the conjugate on gold electrodes in a dense monolayer results in electrodes with a high binding capacity for biotinylated molecules as well as good electron transfer properties. The application potential of such electrodes for bioelectrochemical systems is demonstrated by electrochemical reduction of hydrogen peroxide under mild conditions catalysed by a bound biotin-microperoxidase MP11 conjugate.
Padeste
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Laboratory for Micro- and Nanotechnology, Paul Scherrer Institut, Villigen PSI, Switzerland. celestino.padeste@psi.ch
Grubelnik
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Tiefenauer
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D001360:Avidin / Q000031:analogs & derivatives / Q000138:chemical synthesis / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D001710:Biotin; D004563:Electrochemistry; D004579:Electron Transport; D004800:Enzymes, Immobilized; D005296:Ferrous Compounds / Q000138:chemical synthesis / Q000737:chemistry; D006046:Gold; D006861:Hydrogen Peroxide; D000075163:Metallocenes; D008956:Models, Chemical; D010084:Oxidation-Reduction; D010544:Peroxidases
D004800:Enzymes, Immobilized; D005296:Ferrous Compounds; D000075163:Metallocenes; D001360:Avidin; D001710:Biotin; D006046:Gold; D006861:Hydrogen Peroxide; D010544:Peroxidases; C021799:microperoxidase; C004998:ferrocene