title: Innate immune mechanisms in the pathogenesis of systemic lupus erythematosus (SLE).
abstract: Immune imbalance in SLE increases the susceptibility to infectious diseases. The aim of this study was to analyze several mechanisms related to non-specific immunity in this autoimmune disorder. We studied in vivo CD11b expression, phagocytosis, and chemotaxis in polymorphonuclear cells (PMN) from SLE patients. All tests were also performed under hrIL-8 stimulating conditions and analyzed by flow cytometry. Intracellular leucocyte (monocytes and PMN) enzyme activity was evaluated using specific substrates for cathepsin B and D, collagenase, and oxidative burst by flow cytoenzymology. An exaggerated in vivo CD11b expression was observed on PMN from SLE patients without noticeably in vitro effect upon hrIL-8. Similarly both, phagocytosis and chemotaxis were diminished and showed no response to hrIL-8 stimulation. The opposite was found in PMN from controls. Intracellular enzyme activity was comparable between groups as far as cathepsin B and D are concerned. A tendency of decreased oxidative-burst induction was noted in monocytes and PMN from SLE patients, whereas collagenase activity was found clearly increased in both leucocyte subpopulations. Our results may represent a deficient ability of the innate immune mechanisms for the clearance of infectious agents, immune complexes, satisfactory resolution of inflammatory processes and tissue repair in SLE.
authors: Department of Immunology and Rheumatology, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Vasco de Quiroga 15, Tlalpan 14000, D.F., México, Mexico.
authors: H
authors: H
authors: de la Fuente
authors: Y
authors: Y
authors: Richaud-Patin
authors: J
authors: J
authors: Jakez-Ocampo
authors: R
authors: R
authors: González-Amaro
authors: L
authors: L
authors: Llorente
mesh_terms: D000293:Adolescent; D000328:Adult; D002401:Cathepsin B / Q000378:metabolism; D002402:Cathepsin D / Q000378:metabolism; D002634:Chemotaxis, Leukocyte; D017364:Collagenases / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007113:Immunity, Innate; D016209:Interleukin-8 / Q000494:pharmacology; D008180:Lupus Erythematosus, Systemic / Q000201:enzymology / Q000276:immunology*; D016177:Macrophage-1 Antigen / Q000032:analysis; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000201:enzymology / Q000276:immunology* / Q000378:metabolism; D009504:Neutrophils / Q000201:enzymology / Q000276:immunology* / Q000378:metabolism; D010587:Phagocytosis; D011994:Recombinant Proteins / Q000494:pharmacology; D016897:Respiratory Burst
chemical_list: D016209:Interleukin-8; D016177:Macrophage-1 Antigen; D011994:Recombinant Proteins; D002401:Cathepsin B; D002402:Cathepsin D; D017364:Collagenases