IgA production without mu or delta chain expression in developing B cells.
Surface, membrane-bound, immunoglobulin M (IgM) or IgD expression early in B cell ontogeny is considered essential for the differentiation of antibody-producing cells in mammals; only in IgM+ B cells is the heavy chain locus rearranged to express antibodies of other classes. We show here that IgA is selectively expressed in muMT mice, which lack IgM or IgD expression and have a pro-B cell developmental block. muMT IgA binds proteins of commensal intestinal bacteria and is weakly induced by Salmonella infection, although not through conventional immunization. This muMT IgA pathway requires extrasplenic peripheral lymphoid tissues and may be an evolutionarily primitive system in which immature B cells switch to IgA production at peripheral sites.
Macpherson
A J
AJ
Institute of Experimental Immunology, Universitätsspital, Schmelzbergstrasse 12, CH8091 Zürich, Switzerland. amacpher@pathol.unizh.ch
Lamarre
A
A
McCoy
K
K
Harriman
G R
GR
Odermatt
B
B
Dougan
G
G
Hengartner
H
H
Zinkernagel
R M
RM
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D007070:Immunoglobulin A / Q000096:biosynthesis* / Q000097:blood; D007072:Immunoglobulin D / Q000032:analysis; D007138:Immunoglobulin delta-Chains / Q000235:genetics / Q000276:immunology*; D007148:Immunoglobulin mu-Chains / Q000235:genetics / Q000276:immunology*; D007413:Intestinal Mucosa / Q000276:immunology; D008221:Lymphoid Tissue / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D013154:Spleen / Q000276:immunology
D007070:Immunoglobulin A; D007072:Immunoglobulin D; D007138:Immunoglobulin delta-Chains; D007148:Immunoglobulin mu-Chains