Revised sequence and expression of cyclin B cDNA from the starfish Asterina pectinifera.
Cyclin B cDNA was cloned from the ovary of the starfish Asterina pectinifera and analyzed by RT-PCR and 3'- and 5'-RACE techniques. The cDNA consists of a 0.13-kb upstream untranslated region, a 1.22-kb coding region, and a 0.86-kb downstream untranslated region. The open reading frame encoded a polypeptide of 404 amino acid residues with a calculated molecular weight of 45,692. All the characteristic sequences, such as destruction and cyclin boxes, cyclin B motif, and cytoplasmic retention and nuclear export signals, were found in the newly cloned cyclin B cDNA. The deduced amino acid sequence of the cyclin B cDNA was highly homologous in the middle and carboxy terminal regions to that from mature eggs of the same organism, but quite different in the amino terminal region. Evidence was obtained which suggested that this cyclin B is expressed in immature and maturing oocytes and is the same as that cloned from mature eggs.
Miyake
Y
Y
Department of Bioscience and Biotechnology, Faculty of Engineering, Okayama University, Tsushima-Naka, Japan.
Deshimaru
S
S
Toraya
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D019926:Cyclin B / Q000737:chemistry / Q000235:genetics*; D018076:DNA, Complementary; D005260:Female; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010053:Ovary / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011494:Protein Kinases / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D013215:Starfish
D019926:Cyclin B; D018076:DNA, Complementary; D012333:RNA, Messenger; D011994:Recombinant Proteins; D011494:Protein Kinases; C102333:histone H1 kinase