Genetic material is present in cadaveric dermis and cadaveric fascia lata.
OBJECTIVE
We determined whether genetic material is present in the commercially processed cadaveric allografts used in sling surgery.


METHODS
We evaluated 16 samples from 2 commercial sources of human allograft, including 8 each of freeze-dried gamma irradiated cadaveric fascia lata and acellular cadaveric dermis. Fresh human rectus fascia and sterile saline served as positive and negative controls, respectively. All samples underwent a standard proteinase K/sodium dodecyl sulfate/phenol extraction technique to isolate DNA. Polymerase chain reaction was done to amplify the retrieved DNA material, spectrophotometry to quantify DNA concentration and agarose gel electrophoresis to determine the size of DNA fragments.


RESULTS
Of the 16 samples tested from 2 commercial sources of human allograft fascia 14 (87.5%) contained DNA. Mean DNA concentration plus or minus standard error was 258.3 +/- 80.1 and 272.8 +/- 168.8 microg./gm. tissue for cadaveric fascia lata and cadaveric dermis, respectively. Polymerase chain reaction amplified DNA segments of 2,000 bp from 1 of each of the 8 samples of cadaveric fascia lata and cadaveric dermis.


CONCLUSIONS
Freeze-dried gamma irradiated cadaveric fascia lata and acellular cadaveric dermis contained intact DNA.
Choe
J M
JM
Urodynamics and Continence Center, Division of Urology, University of Cincinnati Medical Center, Cincinnati, Ohio, USA.
Bell
T
T
D001705:Bioprosthesis; D002102:Cadaver; D004247:DNA / Q000032:analysis*; D020405:Dermis / Q000737:chemistry* / Q000637:transplantation; D004586:Electrophoresis; D005206:Fascia Lata / Q000737:chemistry* / Q000637:transplantation; D005260:Female; D005612:Freeze Drying; D015870:Gene Expression; D006801:Humans; D016133:Polymerase Chain Reaction; D012016:Reference Values; D012680:Sensitivity and Specificity; D013718:Tensile Strength; D014015:Tissue Banks; D014184:Transplantation, Homologous; D014549:Urinary Incontinence / Q000601:surgery
D004247:DNA