title: In vitro and in vivo electron transfer to the triheme cytochrome subunit bound to the photosynthetic reaction center complex in the purple bacterium Rhodovulum sulfidophilum.
abstract: The cytochrome subunit bound to the photosynthetic reaction center (RC) complex in Rhodovulum sulfidophilum lacks one heme-binding motif (CXXCH) out of four motifs found in other purple bacteria resulting in the absence of the most distal heme from the RC-core complex (S. Masuda et al., J. Biol. Chem. 274 (1999) 10795). Cytochrome c(2), which acts as the electron donor to the RC was purified, and its gene was cloned and sequenced. The redox midpoint potential of cytochrome c(2) was determined to be E(m)=357 mV. The photo-oxidation and re-reduction of purified cytochrome c(2) were observed in the presence of membrane preparations. Flash-induced photo-oxidation and re-reduction of the RC-bound cytochrome were also observed in intact cells. Despite the unusual nature of the RC-bound cytochrome subunit, the cyclic electron transfer system in Rdv. sulfidophilum was shown to be similar to those in other purple bacteria.
authors: Department of Biology, Tokyo Metropolitan University, Tokyo, Japan. yoshida-makoto@c.metro-u.ac.jp
authors: M
authors: M
authors: Yoshida
authors: S
authors: S
authors: Masuda
authors: K V
authors: KV
authors: Nagashima
authors: A
authors: A
authors: Verméglio
authors: K
authors: K
authors: Shimada
authors: K
authors: K
authors: Matsuura
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D002338:Carotenoids / Q000737:chemistry; D003001:Cloning, Molecular; D003574:Cytochrome c Group / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics; D045362:Cytochromes c2; D004579:Electron Transport; D006217:Halobacterium / Q000737:chemistry* / Q000235:genetics; D006418:Heme / Q000737:chemistry*; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010782:Photolysis; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry*; D016415:Sequence Alignment; D013053:Spectrophotometry
chemical_list: D003574:Cytochrome c Group; D045322:Photosynthetic Reaction Center Complex Proteins; D002338:Carotenoids; D006418:Heme; D045362:Cytochromes c2