title: Soluble CD23 in cerebrospinal fluid: a marker of AIDS-related non-Hodgkin's lymphoma in the brain.
abstract: BACKGROUND
  AIDS-related non-Hodgkin's lymphoma (NHL) includes systemic lymphomas, often with brain involvement, and primary central nervous system (CNS) lymphomas.
  
  
  OBJECTIVE
  To examine if measurement of soluble CD23 (sCD23) in cerebrospinal fluid (CSF) is useful in the diagnosis and follow-up of AIDS-related NHL.
  
  
  METHODS
  sCD23 was measured by enzyme-linked immunosorbent assay and EBV DNA by nested polymerase chain reaction for a group of 134 patients. The NHL group included 14 patients with primary HIV-1 CNS lymphoma, 12 patients with brain involvement of systemic HIV-1 NHL and 10 patients with systemic HIV-1 NHL without brain involvement. These were compared with HIV-1-infected patients with cerebral toxoplasmosis (19), progressive multifocal leukoencephalitis (PML; 8) and AIDS-related dementia (17) and with asymptomatic HIV-1 carriers (54) and uninfected individuals (50). The levels of sCD23 were compared with the presence of Epstein-Barr virus (EBV) DNA in CSF.
  
  
  RESULTS
  Significantly higher levels of sCD23 were found in the CSF of the patients with brain lymphoma than in those with systemic NHL (P < 0.002) or with cerebral toxoplasmosis, PML and AIDS-related dementia (P < 0.0001). The sensitivity and specificity of sCD23 in CSF as a marker for detection of brain NHL were 77% and 94%, respectively. High levels of sCD23 were found in CSF from patients with brain NHL independently of the presence (18 out of 26) or absence (8 out of 26) of EBV DNA.
  
  
  CONCLUSIONS
  The sCD23 in CSF of HIV-1-infected patients may represent an additional, non-invasive marker for diagnosis of brain involvement in AIDS-related NHL.
authors: Division of Infectious Diseases, San Raffaele Hospital, Milan, Italy.
authors: S
authors: S
authors: Bossolasco
authors: A
authors: A
authors: Nilsson
authors: A
authors: A
authors: de Milito
authors: A
authors: A
authors: Lazzarin
authors: A
authors: A
authors: Linde
authors: P
authors: P
authors: Cinque
authors: F
authors: F
authors: Chiodi
mesh_terms: D015415:Biomarkers; D001921:Brain / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D016483:Lymphoma, AIDS-Related / Q000134:cerebrospinal fluid / Q000276:immunology*; D017455:Receptors, IgE / Q000032:analysis*; D012995:Solubility
chemical_list: D015415:Biomarkers; D017455:Receptors, IgE