Carotenoids as antioxidants: spin trapping EPR and optical study.
The role of several natural and synthetic carotenoids as scavengers of free radicals was studied in homogeneous solutions. A set of free radicals: *OH, *OOH, and *CH(3) were generated by using the Fenton reaction in dimethyl sulfoxide. It was shown that the spin trapping technique is more informative than optical methods for the experimental conditions under study. 5,5-Dimethyl-pyrroline-N-oxide (DMPO) and N-tert-butyl-alpha-phenylnitrone (PBN) were used as spin traps for the EPR studies. The results show that the scavenging ability of the carotenoids towards radical *OOH correlates with their redox properties.
Polyakov
N E
NE
Institute of Chemical Kinetics and Combustion, Novosibirsk, Russia.
Kruppa
A I
AI
Leshina
T V
TV
Konovalova
T A
TA
Kispert
L D
LD
D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D002338:Carotenoids / Q000737:chemistry / Q000494:pharmacology*; D004121:Dimethyl Sulfoxide / Q000378:metabolism; D004578:Electron Spin Resonance Spectroscopy; D016166:Free Radical Scavengers / Q000378:metabolism; D005609:Free Radicals / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000737:chemistry; D007501:Iron / Q000737:chemistry; D019097:Spin Trapping
D000975:Antioxidants; C045076:Fenton's reagent; D016166:Free Radical Scavengers; D005609:Free Radicals; D002338:Carotenoids; D006861:Hydrogen Peroxide; D007501:Iron; D004121:Dimethyl Sulfoxide