title: Biosynthetic response of cultured articular chondrocytes to mechanical vibration.
abstract: The objective of this study was to determine the effects of mechanical vibration loading on DNA and proteoglycan syntheses in cultured rabbit articular chondrocytes. Chondrocyte culture plates were placed in a vibratory apparatus and subjected to a mechanical vibratory load at various frequencies and periods in culture. Mechanical vibration was applied at a sinusoidal waveform of 1.4 g acceleration with frequencies of 200, 300, 400, 800, and 1600 Hz. 3H-Thymidine and H2(35)SO4 incorporation were used to detect radiolabeled DNA and proteoglycan syntheses, respectively. A frequency of 300 Hz showed a time-dependent augmentation of DNA synthesis and gave a maximal increase at day 3 with periodic vibration (8 h per day) and at 72 h or longer with continuous vibration. It also promoted proteoglycan synthesis in long-term culture (from 3 to 15 days) by periodic vibration. However. frequencies above 400 Hz suppressed biosynthesis. These results suggest that mechanical vibration at certain frequencies may modulate biosynthetic response of articular chondrocytes.
authors: Department of Orthopaedic Surgery, Nagoya City University Medical School, Nagoya, Japan. liu@med.nagoya-cu.ac.jp
authors: J
authors: J
authors: Liu
authors: I
authors: I
authors: Sekiya
authors: K
authors: K
authors: Asai
authors: T
authors: T
authors: Tada
authors: T
authors: T
authors: Kato
authors: N
authors: N
authors: Matsui
mesh_terms: D000818:Animals; D002358:Cartilage, Articular / Q000166:cytology; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology / Q000378:metabolism*; D004247:DNA / Q000096:biosynthesis; D006621:Hip Joint / Q000166:cytology; D007719:Knee Joint / Q000166:cytology; D008297:Male; D010812:Physical Stimulation; D011509:Proteoglycans / Q000096:biosynthesis; D011817:Rabbits; D013462:Sulfur Radioisotopes; D013464:Sulfuric Acids / Q000493:pharmacokinetics; D013936:Thymidine / Q000493:pharmacokinetics; D014316:Tritium; D014732:Vibration
chemical_list: D011509:Proteoglycans; D013462:Sulfur Radioisotopes; D013464:Sulfuric Acids; D014316:Tritium; D004247:DNA; C033158:sulfuric acid; D013936:Thymidine