title: Recruitment of HAT complexes by direct activator interactions with the ATM-related Tra1 subunit.
abstract: Promoter-specific recruitment of histone acetyltransferase activity is often critical for transcriptional activation. We present a detailed study of the interaction between the histone acetyltransferase complexes SAGA and NuA4, and transcription activators. We demonstrate by affinity chromatography and photo-cross-linking label transfer that acidic activators directly interact with Tra1p, a shared subunit of SAGA and NuA4. Mutations within the COOH-terminus of Tra1p disrupted its interaction with activators and resulted in gene-specific transcriptional defects that correlated with lowered promoter-specific histone acetylation. These data demonstrate that the essential Tra1 protein serves as a common target for activators in both SAGA and NuA4 acetyltransferases.
authors: Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biology, Department of Chemistry, The Pennsylvania State University, 306 Althouse Laboratory, University Park, PA 16802, USA.
authors: C E
authors: CE
authors: Brown
authors: L
authors: L
authors: Howe
authors: K
authors: K
authors: Sousa
authors: S C
authors: SC
authors: Alley
authors: M J
authors: MJ
authors: Carrozza
authors: S
authors: S
authors: Tan
authors: J L
authors: JL
authors: Workman
mesh_terms: D000107:Acetylation; D000123:Acetyltransferases / Q000737:chemistry / Q000378:metabolism*; D000483:Alleles; D023081:CCAAT-Binding Factor / Q000378:metabolism; D003432:Cross-Linking Reagents; D004268:DNA-Binding Proteins / Q000378:metabolism; D005656:Fungal Proteins / Q000378:metabolism; D051548:Histone Acetyltransferases; D006657:Histones / Q000378:metabolism; D009154:Mutation; D011401:Promoter Regions, Genetic; D011494:Protein Kinases / Q000378:metabolism; D021122:Protein Subunits; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D035381:TATA-Binding Protein Associated Factors; D013696:Temperature; D015534:Trans-Activators / Q000378:metabolism*; D035362:Transcription Factor TFIID; D014157:Transcription Factors / Q000378:metabolism; D015533:Transcriptional Activation; D015003:Yeasts / Q000235:genetics / Q000378:metabolism
chemical_list: C090575:ADA2 protein, S cerevisiae; D023081:CCAAT-Binding Factor; D003432:Cross-Linking Reagents; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; C061437:HAP4 protein, S cerevisiae; D006657:Histones; D021122:Protein Subunits; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D029701:Saccharomyces cerevisiae Proteins; C116141:TAF9 protein, S cerevisiae; D035381:TATA-Binding Protein Associated Factors; D015534:Trans-Activators; D035362:Transcription Factor TFIID; D014157:Transcription Factors; D000123:Acetyltransferases; D051548:Histone Acetyltransferases; D011494:Protein Kinases