Expression of two novel zebrafish iroquois homologues (ziro1 and ziro5) during early development of axial structures and central nervous system.
Previously, we reported a zebrafish iroquois gene, ziro3, and its expression during early embryogenesis (Mech. Dev. 87 (1999) 165). In the present study, we have isolated two novel zebrafish iroquois genes, ziro1 and ziro5, homologs of mouse Irx1 and mouse Irx5, respectively. The expression of both genes is initiated in dorsal neuroectoderm and mesoderm during gastrulation. Later, their expression appears in the central nervous system (CNS), excluding the telencephalon and most of the diencephalon. ziro1 expression is complementary to that of ziro3 in the notochord and later in the gut. In contrast, ziro5 expression mostly overlaps with that of ziro3. Interestingly, all three iroquois zebrafish genes are expressed in the notochord while only Irx3 is active in the mouse notochord. Their expression in later stages of embryogenesis was also compared.
Wang
X
X
Department of Biological Sciences, National University of Singapore, 119260, Singapore, Singapore.
Emelyanov
A
A
Sleptsova-Friedrich
I
I
Korzh
V
V
Gong
Z
Z
D000595:Amino Acid Sequence; D000818:Animals; D002490:Central Nervous System / Q000196:embryology*; D018076:DNA, Complementary / Q000378:metabolism; D004027:Diencephalon / Q000378:metabolism; D018398:Homeodomain Proteins / Q000096:biosynthesis*; D017403:In Situ Hybridization; D051379:Mice; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009672:Notochord / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013687:Telencephalon / Q000378:metabolism; D013997:Time Factors; D014157:Transcription Factors / Q000096:biosynthesis*; D015027:Zebrafish; D029961:Zebrafish Proteins
D018076:DNA, Complementary; D018398:Homeodomain Proteins; C501592:Irx1 protein, mouse; C501599:Irx3 protein, mouse; C501601:Irx5 protein, mouse; D014157:Transcription Factors; D029961:Zebrafish Proteins; C501593:irx1a protein, zebrafish; C459599:irx1b protein, zebrafish