Separation and characterization of the 1,3-propanediol and glycerol dehydrogenase activities from Clostridium butyricum E5 wild-type and mutant D.
OBJECTIVE
Clostridium butyricum E5 wild-type and mutant E5-MD were cultivated in chemostat culture on glycerol in order to compare the properties of two key enzymes of glycerol catabolism, i.e. propanediol and glycerol dehydrogenase.


RESULTS
These two enzymes, which belong to the dha regulon, were separated by gel filtration. Both dehydrogenase activities displayed similar properties, such as pH optimum values, specificity towards physiological substrates and dependence on Mn2+. Both strains accumulate glycerol at high levels.


CONCLUSIONS
The mutant D strain contained a propanediol dehydrogenase activity which had a low affinity for its physiological substrate, leading to the conclusion that this strain would seem more resistant to the toxic effect of 3-hydroxypropionaldehyde than the wild-type.


CONCLUSIONS
These properties make Cl. butyricum mutant D strain the best candidate so far to be used as a biotechnological agent for the bioconversion of glycerol to 1,3-propanediol.
Malaoui
H
H
Laboratoire de Biochimie des Bactéries Gram +, Université Henri Poincaré, Vandoeuvre lès Nancy cédex, France.
Marczak
R
R
D000426:Alcohol Dehydrogenase; D000429:Alcohol Oxidoreductases / Q000302:isolation & purification*; D002413:Cations, Divalent; D002414:Cations, Monovalent; D002850:Chromatography, Gel; D003013:Clostridium / Q000201:enzymology* / Q000235:genetics; D004338:Drug Combinations; D007700:Kinetics; D009154:Mutation; D009821:Oils; D010636:Phenols; D013379:Substrate Specificity; D013401:Sugar Alcohol Dehydrogenases / Q000302:isolation & purification*
D002413:Cations, Divalent; D002414:Cations, Monovalent; D004338:Drug Combinations; D009821:Oils; C027222:P & S Liquid; D010636:Phenols; D000429:Alcohol Oxidoreductases; D013401:Sugar Alcohol Dehydrogenases; D000426:Alcohol Dehydrogenase; C052370:1,3-propanediol dehydrogenase; C020672:glycerol dehydrogenase