Luminescence investigations of redox cycling of adriamycin.
The light emission from the adriamycin + Co2+ + H2O2 system has been studied. Chemiluminescence, fluorescence and absorption spectra were measured. The fluorescence spectra were time-dependent exhibiting maxima at 555, 590 and 645 nm. The chemiluminescence spectra consist of four bands with maxima at around 460-500, 550-580, 640 and 700 nm. Free radical reaction inhibitors, (1)O2-quenchers and catalase inhibited the light emission indicating that hydroxyl radical, superoxide anion radical and singlet oxygen are generated during the redox cycling of adriamycin. Chemiluminescence studies revealed that adriamycin undergoes chemiexcitation under our experimental conditions.
Kruk
I
I
Institute of Physics, Technical University of Szczecin, Poland. kruk@arcadia.tuniv.szczecin.pl
Michalska
T
T
Kładny
J
J
Kubera-Nowakowska
L
L
D000970:Antineoplastic Agents / Q000032:analysis / Q000737:chemistry*; D004317:Doxorubicin / Q000032:analysis / Q000737:chemistry*; D008163:Luminescent Measurements; D010084:Oxidation-Reduction; D013050:Spectrometry, Fluorescence; D013054:Spectrophotometry, Atomic
D000970:Antineoplastic Agents; D004317:Doxorubicin