Antigenic properties and experimental transmission to several fish species of a marine birnavirus isolated from sole (Solea senegalensis).
A cross-neutralization test was used to study the antigenic relationship of an aquabirnavirus isolated from sole (Solea senegalensis), named solevirus, and several infectious pancreatic necrosis virus (IPNV) strains. Solevirus was antigenically similar to IPNV strain Sp. Transmission of the solevirus to other fish species has been determined by inoculation to freshwater and marine fish species (two salmonids and gilt-head seabream). A higher pathogenicity was obtained for the marine fish species, although solevirus caused an asymptomatic infection in all species tested, as demonstrated by the detection of viral RNA and of viral antigens in fish leucocytes, respectively, using polymerase chain reaction (PCR) and flow cytometry (FC).
Perez-Prieto
S
S
Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas, Velázquez 144, 28006 Madrid, Spain.
Garcia-Rosado
E
E
Rodriguez
S
S
Castro
D
D
Borrego
J J
JJ
D000818:Animals; D000956:Antigens, Viral / Q000276:immunology*; D017756:Aquaculture; D018059:Birnaviridae / Q000302:isolation & purification / Q000472:pathogenicity; D018175:Birnaviridae Infections / Q000635:transmission / Q000662:veterinary*; D002460:Cell Line; D002469:Cell Separation / Q000662:veterinary; D004196:Disease Outbreaks / Q000662:veterinary; D004198:Disease Susceptibility; D005393:Fish Diseases / Q000635:transmission* / Q000821:virology; D005412:Flatfishes; D005434:Flow Cytometry / Q000662:veterinary; D009500:Neutralization Tests / Q000662:veterinary; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012487:Salmonidae / Q000821:virology*
D000956:Antigens, Viral