Fibroblasts require protein kinase C activation to respond to hyaluronan with increased locomotion.
Hyaluronan (HA) stimulates the motility of some but not all cell types. Here, we show that HA-promoted random motility of ras-transformed 10T1/2 (C3) fibroblasts requires activation of protein kinase C and is associated with rapid uptake of HA in a CD44 and RHAMM-dependent manner. The addition of HA to parental 10T1/2 fibroblasts (parental cells) does not stimulate random motility, but these cells can be 'primed' to respond to HA by treatment with the phorbol ester, PMA, for 4-6 h. This effect of PMA requires protein synthesis, PKC activity and is associated with enhanced uptake of HA. These results suggest that the ability of cells to respond to HA is regulated by a protein kinase C-dependent process that may promote uptake of HA.
Hall
C L
CL
Manitoba Institute of Cell Biology, University of Manitoba, Winnipeg, Manitoba, Canada.
Collis
L A
LA
Bo A
J
J
Lange
L
L
McNicol
A
A
Gerrard
J M
JM
Turley
E A
EA
D002460:Cell Line; D002461:Cell Line, Transformed; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology*; D004075:Diglycerides / Q000096:biosynthesis; D004789:Enzyme Activation; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D018960:Hyaluronan Receptors / Q000096:biosynthesis; D006820:Hyaluronic Acid / Q000378:metabolism* / Q000494:pharmacology; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D018631:ras Proteins / Q000235:genetics / Q000378:metabolism
D004075:Diglycerides; D018960:Hyaluronan Receptors; D006820:Hyaluronic Acid; D011493:Protein Kinase C; D018631:ras Proteins; D013755:Tetradecanoylphorbol Acetate