Simultaneous determination of the HIV-protease inhibitors indinavir, amprenavir, ritonavir, saquinavir and nelfinavir in human plasma by reversed-phase high-performance liquid chromatography.
A rapid, simple and sensitive high-performance liquid chromatographic (HPLC) assay has been developed for the simultaneous quantification of the HIV-protease inhibitors indinavir, amprenavir, ritonavir, saquinavir and nelfinavir in human plasma. The method involved the solid-phase extraction of the five drugs and the internal standard (I.S., verapamil) from 400 microl of human plasma. The HPLC analysis used a reversed-phase C18 analytical column and a mobile phase consisting of a gradient with 15 mM phosphate buffer (pH 5.75)-acetonitrile and UV monitoring. The method was linear over the therapeutic concentration range for the five HIV-protease inhibitors. The accuracy of the method ranged from 98.2 to 106.7% and the precision values ranged from 1.4 to 8.1% for intra-day precision and from 3.1 to 6.4% for the inter-day values.
Sarasa-Nacenta
M
M
Clinical Pharmacology Unit, Hospital Clínic, Barcelona, Spain. msarasa@clinic.ub.es
López-Púa
Y
Y
Mallolas
J
J
Blanco
J L
JL
Gatell
J M
JM
Carné
X
X
D002219:Carbamates; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005663:Furans; D015658:HIV Infections / Q000097:blood; D017320:HIV Protease Inhibitors / Q000097:blood*; D006801:Humans; D019469:Indinavir / Q000097:blood; D019888:Nelfinavir / Q000097:blood; D012015:Reference Standards; D015203:Reproducibility of Results; D019438:Ritonavir / Q000097:blood; D019258:Saquinavir / Q000097:blood; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D013449:Sulfonamides / Q000097:blood
D002219:Carbamates; D005663:Furans; D017320:HIV Protease Inhibitors; D013449:Sulfonamides; C095108:amprenavir; D019469:Indinavir; D019888:Nelfinavir; D019258:Saquinavir; D019438:Ritonavir