title: Ongoing twin pregnancy after ICSI of PESA-retrieved spermatozoa into in-vitro matured oocytes: case report.
abstract: The recovery of immature oocytes from unstimulated ovaries followed by in-vitro maturation (IVM) is an attractive alternative to conventional IVF in the treatment of female infertility. Similarly, surgical recovery of spermatozoa from the epididymis by percutaneous sperm aspiration (PESA) has simplified the retrieval of the male gamete in treatment of men with obstructive azoospermia. We report the first ongoing clinical twin pregnancy resulting from intracytoplasmic sperm injection (ICSI) of spermatozoa retrieved by PESA into IVM oocytes. In the treatment of a 24-year old woman, 12 immature oocytes were retrieved. Six oocytes matured (maturation rate 50%) after 24-hour incubation and were inseminated by ICSI. Four oocytes had two pronuclei (fertilization rate 67%) and 3 good quality embryos were transferred. A viable twin pregnancy was confirmed by ultrasound scan. This report illustrates the use of a combination of less invasive assisted reproductive techniques in overcoming barriers to infertility.
authors: Department of Obstetrics and Gynecology, McGill Reproductive Center, Royal Victoria Hospital F6.58, McGill University, 687 Pine Avenue West, Montreal, Quebec, H3A 1A1, Canada. ahmad.kamal@muhc.mcgill.ca
authors: A K
authors: AK
authors: Abdul-Jalil
authors: T J
authors: TJ
authors: Child
authors: S
authors: S
authors: Phillips
authors: N
authors: N
authors: Dean
authors: S
authors: S
authors: Carrier
authors: S L
authors: SL
authors: Tan
mesh_terms: D000328:Adult; D004624:Embryo Transfer; D004822:Epididymis / Q000166:cytology; D005260:Female; D006801:Humans; D007248:Infertility, Male / Q000628:therapy*; D008297:Male; D009865:Oocytes / Q000502:physiology; D011247:Pregnancy; D020554:Sperm Injections, Intracytoplasmic; D013094:Spermatozoa; D013396:Suction; D020858:Tissue and Organ Harvesting / Q000379:methods*; D014427:Twins; D016216:Ultrasonography, Prenatal