title: In vivo PC3 overexpression by retroviral vector affects cell differentiation of rat cortical precursors.
abstract: The PC3 gene is a marker of dividing neuroepithelial (NE) cells. We transduced single cortical precursors of the ventricular zone (VZ) with a PC3-carrying retroviral vector at E16 stage, and analysed the effects of transgene expression on their progeny in 3-week-old animals. Unlike control-transduced cells, all viable PC3-transduced cells remained close to the ventricle and displayed a round-shaped, undifferentiated morphology.
authors: Dipartimento di Fisiologia e Biochimica, Sezione di Biologia Cellulare e dello Sviluppo, Università di Pisa, via Carducci 13, 56010 Ghezzano, Pisa, Italy.
authors: P
authors: P
authors: Malatesta
authors: P
authors: P
authors: Sgadò
authors: L
authors: L
authors: Caneparo
authors: G
authors: G
authors: Barsacchi
authors: F
authors: F
authors: Cremisi
mesh_terms: D000818:Animals; D016282:Aspartic Acid Endopeptidases / Q000235:genetics*; D002454:Cell Differentiation / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D002540:Cerebral Cortex / Q000166:cytology* / Q000196:embryology*; D005260:Female; D018507:Gene Expression Regulation, Developmental; D015967:Gene Expression Regulation, Viral; D005822:Genetic Vectors; D009474:Neurons / Q000166:cytology / Q000502:physiology; D011247:Pregnancy; D043484:Proprotein Convertases; D051381:Rats; D017207:Rats, Sprague-Dawley; D012190:Retroviridae / Q000235:genetics; D013234:Stem Cells / Q000166:cytology / Q000502:physiology
chemical_list: D043484:Proprotein Convertases; D016282:Aspartic Acid Endopeptidases