Mass spectrometric evidence for the deficiency in the dark-color-inducing hormone,.
A factor present in the brain and corpus cardiacum responsible for the induction of dark colour in Locusta migratoria was recently isolated and identified from the corpora cardiaca of normally pigmented locusts. The purification of this factor, designated as [His7]-corazonin was monitored using an albino mutant from a laboratory colony of an Okinawa (Japan) strain. In this study, we provide unequivocal mass spectrometric evidence that the brain and the corpora cardiaca of this albino Locusta mutant are deficient in [His7]-corazonin. Previously, [His7]-corazonin was shown to be responsible for the induction of dark body colour patterns as observed in crowded locusts. Using nanoflow-liquid chromatography-mass spectrometry, we demonstrated that this dark colour-inducing hormone is, however, present in the corpora cardiaca of solitary locusts (Schistocerca gregaria). Arch.
Baggerman
G
G
Zoological Institute, K.U.Leuven, Leuven, Belgium.
Clynen
E
E
Mazibur
R
R
Veelaert
D
D
Breuer
M
M
De Loof
A
A
Tanaka
S
S
Schoofs
L
L
D000818:Animals; D000918:Antibody Specificity; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid; D003116:Color; D006110:Grasshoppers / Q000737:chemistry*; D007150:Immunohistochemistry; D019476:Insect Proteins; D013058:Mass Spectrometry; D009479:Neuropeptides / Q000032:analysis*; D009490:Neurosecretory Systems / Q000737:chemistry*
D019476:Insect Proteins; D009479:Neuropeptides; C060602:corazonin protein, insect