Effect of disaggregation on calpain activity in explants from rat thyroid glands.
We studied the regulation of calpains in explants from rat thyroid glands. Collagenase disaggregation decreased proteinase activity in thyrocytes. It was hypothesized that this effect is mediated by heterotrimeric G proteins, protein kinase C, and tyrosine kinase, but not via Ca(2+) and cAMP-dependent signal pathway.
Stroev
E A
EA
Department of Biochemistry, I. P. Pavlov Ryazan State Medical University.
Makarova
V G
VG
Ryazanova
E A
EA
Plyakhin
I V
IV
D000818:Animals; D002118:Calcium / Q000378:metabolism; D002154:Calpain / Q000378:metabolism*; D000242:Cyclic AMP / Q000378:metabolism; D020962:Heterotrimeric GTP-Binding Proteins / Q000502:physiology; D066298:In Vitro Techniques; D008297:Male; D011493:Protein Kinase C / Q000378:metabolism; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D051381:Rats; D015398:Signal Transduction; D013961:Thyroid Gland / Q000166:cytology / Q000201:enzymology*
D000242:Cyclic AMP; D011505:Protein-Tyrosine Kinases; D011493:Protein Kinase C; D002154:Calpain; D020962:Heterotrimeric GTP-Binding Proteins; D002118:Calcium