Infrared-thermographic screening of the activity and enantioselectivity of enzymes.
The infrared radiation caused by the heat of reaction of an enantioselective enzyme-catalyzed transformation can be detected by modern photovoltaic infrared (IR)-thermographic cameras equipped with focal-plane array detectors. Specifically, in the lipase-catalyzed enantioselective acylation of racemic 1-phenylethanol, the (R)- and (S)-substrates are allowed to react separately in the wells of microtiter plates, the (R)-alcohol showing hot spots in the IR-thermographic images. Thus, highly enantioselective enzymes can be identified at kinetic resolution.
Reetz
M T
MT
Max-Planck-Institut für Kohlenforschung, Germany. reetz@mpi-muelheim.mpg.de
Hermes
M
M
Becker
M H
MH
D001709:Biotechnology; D002384:Catalysis; D004798:Enzymes / Q000378:metabolism*; D066298:In Vitro Techniques; D007259:Infrared Rays; D007700:Kinetics; D013237:Stereoisomerism; D013817:Thermography / Q000379:methods*
D004798:Enzymes