Conditional gene knockout using Cre recombinase.
Cre recombinase has become an important instrument for achieving precise genetic manipulation in mice. Many of these desired genetic manipulations rely on Cre's ability to direct spatially and temporally specified excision of a predesignated DNA sequence that has been flanked by directly repeated copies of the loxP recombination site. Success in achieving such conditional mutagenesis in mice depends both on the careful design of conditional alleles and on reliable detection of cre gene expression. These procedures include PCR, immunohistochemistry and the use of a recombination-proficient GFP-tagged Cre protein.
Le
Y
Y
Sauer
B
B
D000818:Animals; D001483:Base Sequence; D002469:Cell Separation; D005434:Flow Cytometry; D005821:Genetic Techniques; D007150:Immunohistochemistry; D019426:Integrases / Q000235:genetics* / Q000378:metabolism*; D051379:Mice; D018345:Mice, Knockout; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D011995:Recombination, Genetic; D013234:Stem Cells / Q000166:cytology; D019076:Transgenes; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism*
D011993:Recombinant Fusion Proteins; D014764:Viral Proteins; C045073:Cre recombinase; D019426:Integrases