Direct measurement of HDL cholesterol: method eliminating apolipoprotein E-rich particles.
It has been reported that the existing direct method of high density lipoprotein (HDL) cholesterol measures particles enriched with apolipoprotein E (apoE). The aim of our study was to investigate a new analytical protocol to directly measure HDL cholesterol that eliminates apoE-rich particles. The interactions of four lipoproteins (HDL(3), HDL(2), LDL, and VLDL + chylomicron) with surfactants, divalent cations, sugars, and lectins were investigated. By analyzing sera, HDL(3), and HDL(2), we examined the relationships among the measurements obtained by our protocol, a precipitation method using heparin-MnCl(2), and a commercially available kit for this direct method. A significant difference was found between the direct method and the heparin-MnCl(2) method, but not between our protocol and the heparin-MnCl(2) method. Multiple regression analysis showed that the difference between the direct method and the heparin MnCl(2) method is dependent on sources of apoE-rich HDL. In conclusion, our protocol enables a direct measurement of HDL cholesterol that eliminates apoE-rich particles.
Okada
M
M
Department of Laboratory Medicine, Niigata University School of Medicine, Niigata City, Japan. okadar@med.niigata-u.ac.jp
Matsui
H
H
Ito
Y
Y
Fujiwara
A
A
D001057:Apolipoproteins E / Q000097:blood*; D002374:Catalase; D002413:Cations, Divalent; D011232:Chemical Precipitation; D002712:Chlorides; D008076:Cholesterol, HDL / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D002914:Chylomicrons / Q000097:blood; D006493:Heparin; D006801:Humans; D007202:Indicators and Reagents; D037102:Lectins; D008075:Lipoproteins, HDL / Q000097:blood; D053439:Lipoproteins, HDL2; D053440:Lipoproteins, HDL3; D008077:Lipoproteins, LDL / Q000097:blood; D008079:Lipoproteins, VLDL / Q000097:blood; D015636:Magnesium Chloride; D017895:Manganese Compounds; D011786:Quality Control; D012044:Regression Analysis; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D012680:Sensitivity and Specificity; D002787:Sterol Esterase; D013501:Surface-Active Agents
D001057:Apolipoproteins E; D002413:Cations, Divalent; D002712:Chlorides; D008076:Cholesterol, HDL; D002914:Chylomicrons; D007202:Indicators and Reagents; D037102:Lectins; D008075:Lipoproteins, HDL; D053439:Lipoproteins, HDL2; D053440:Lipoproteins, HDL3; D008077:Lipoproteins, LDL; D008079:Lipoproteins, VLDL; D017895:Manganese Compounds; D013501:Surface-Active Agents; D015636:Magnesium Chloride; D006493:Heparin; D002374:Catalase; D002787:Sterol Esterase; C025340:manganese chloride