Ectopic expression of the Arabidopsis AtMYB23 gene induces differentiation of trichome cells.
The control of epidermal cell fate is a complex molecular process and requires the regulatory activity of different transcription factors. Here, we describe the isolation of a member of the Arabidopsis MYB transcription factor family, AtMYB23, that is involved in trichome development. Expression of the AtMYB23 gene under the control of the viral CaMV 35S promoter causes the development of ectopic trichomes. The formation of ectopic trichomes depends on TRANSPARENT TESTA GLABRA1 but not on GLABRA1. The absence of the negative regulator TRIPTYCHON leads to branching of the ectopic trichomes on cotyledons and the formation of ectopic trichomes in the leaf subepidermal cell layer. The CaMV 35S promoter-controlled expression of AtMYB23 can partially rescue the glabra1 mutant phenotype. Together, the presented data indicate that the AtMYB23 gene has partially overlapping functions with GLABRA1 in controlling the initiation of trichome development.
Kirik
V
V
Institut für Pflanzengenetik und Kulturpflanzenforschung, Corrensstrasse 3, 06466 Gatersleben, Germany.
Schnittger
A
A
Radchuk
V
V
Adler
K
K
Hülskamp
M
M
Bäumlein
H
H
D017360:Arabidopsis / Q000235:genetics* / Q000648:ultrastructure; D029681:Arabidopsis Proteins; D002454:Cell Differentiation; D018076:DNA, Complementary / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics*; D020570:Genes, myb / Q000235:genetics*; D005822:Genetic Vectors; D008855:Microscopy, Electron, Scanning; D010641:Phenotype; D010940:Plant Proteins / Q000096:biosynthesis* / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D020598:Proto-Oncogene Proteins c-myb; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014170:Transformation, Genetic
C511988:ATR1 protein, Arabidopsis; D029681:Arabidopsis Proteins; C511989:CPC protein, Arabidopsis; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D010940:Plant Proteins; D020598:Proto-Oncogene Proteins c-myb; C121067:TTG1 protein, Arabidopsis