Determination of hydrogen sulfide and acid-labile sulfur in animal tissues by gas chromatography and ion chromatography.
A sensitive and reliable method was developed for the determination of hydrogen sulfide and acid-labile sulfur (ALS) in animal tissues using gas chromatography with flame photometric detector (GC-FPD) and ion chromatography (IC). Hydrogen sulfide trapped in alkaline solution was determined by GC-FPD as hydrogen sulfide or by IC as sulfate after oxidation with hydrogen peroxide. Sodium sulfide used as a source of hydrogen sulfide was standardized by IC. Fresh rat liver and heart tissues contained 112.2+/-23.0 and 274.1+/-34.6 nmol/g of ALS respectively. Free hydrogen sulfide was not detected.
Ubuka
T
T
Department of Clinical Nutrition, Kawasaki University of Medical Welfare, Kurashiki, Okayama, Japan. ubukatos@mw.kawasaki-m.ac.jp
Abe
T
T
Kajikawa
R
R
Morino
K
K
D000143:Acids; D000818:Animals; D002849:Chromatography, Gas / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods*; D006862:Hydrogen Sulfide / Q000032:analysis*; D008099:Liver / Q000737:chemistry*; D009206:Myocardium / Q000737:chemistry*; D051381:Rats; D013455:Sulfur / Q000032:analysis*
D000143:Acids; D013455:Sulfur; D006862:Hydrogen Sulfide