Effects of profound hemodilution on small-intestinal wound healing in rabbits.
BACKGROUND
Wound healing is influenced by tissue oxygen tension and blood perfusion, but not by moderate anemia or hemodilution. The effect of perioperative profound hemodilution on small-intestinal wound healing remains unclear.


METHODS
We performed jejunectomy followed by end-to-end anastomosis in rabbits subjected to a variety of perioperative hemodilutions: HD((HES)), hemodiluted with hydroxyethylstarch; HD((P+HES)), hemodiluted with autologous plasma and hydroxyethylstarch; HD((HES))/R, hemodiluted with hydroxyethylstarch and retransfused afterward. Intraoperative hemoglobin levels were 5 g 100 ml(-1). On Postoperative Day 5, the tensile strength (TS) of the anastomosis was measured and histological specimen was obtained. The time courses of hemoglobin, serum albumin (Alb), plasma fibrinogen (Fbg), and plasma activity of factor XIII (F XIII) were measured.


RESULTS
TS in HD((HES))/R (236.0 +/- 52.2 gf) was similar to that in control (266.5 +/- 41.6 gf); however, TS in HD((HES)) (179.8 +/- 17.9 gf) and HD((P+HES)) (165.5 +/- 14.7 gf) decreased significantly. The histological findings in HD((HES))/R were similar to those of control, whereas they demonstrated a delayed healing process in HD((HES)) and HD((P+HES)). Hemoglobin levels were still lower on Postoperative Day 5 in HD((HES)) and HD((P+HES)), but increased to 10.0 g 100 ml(-1) after retransfusion in HD((HES))/R. Hemodilution caused significant decreases in Alb, Fbg, and F XIII, but the values after retransfusion in HD((HES))/R were similar to postoperative values in HD((P+HES)).


CONCLUSIONS
Intraoperative profound hemodilution does not interfere with small-intestinal wound healing as long as postoperative hemoglobin levels were maintained above 10 g 100 ml(-1). Postoperative levels of other plasma constituents may not influence wound healing.
Mandai
R
R
Intensive Care Unit, Shiga University of Medical Science, Shiga, 520-2192, Japan. mandai@belle.shiga-med.ac.jp
Eguchi
Y
Y
Tanaka
M
M
Sai
Y
Y
Nosaka
S
S
D000714:Anastomosis, Surgical; D000818:Animals; D001804:Blood Transfusion, Autologous; D005176:Factor XIII / Q000032:analysis; D005340:Fibrinogen / Q000032:analysis; D006438:Hemodilution / Q000379:methods; D006454:Hemoglobins / Q000032:analysis; D006895:Hydroxyethyl Starch Derivatives / Q000627:therapeutic use; D007421:Intestine, Small / Q000473:pathology / Q000503:physiopathology* / Q000601:surgery*; D007583:Jejunum / Q000601:surgery; D010952:Plasma Substitutes / Q000627:therapeutic use; D011817:Rabbits; D012709:Serum Albumin / Q000032:analysis; D013718:Tensile Strength; D014945:Wound Healing / Q000502:physiology*
D006454:Hemoglobins; D006895:Hydroxyethyl Starch Derivatives; D010952:Plasma Substitutes; D012709:Serum Albumin; D005340:Fibrinogen; D005176:Factor XIII