Ectopic expression of MAFB gene in human myeloma cells carrying (14;20)(q32;q11) chromosomal translocations.
Chromosome 14q +, which represents a chromosomal rearrangement involving the immunoglobulin heavy chain gene (IgH) locus, is a genetic hallmark of human multiple myeloma (MM). Here, we report the identification of (14;20)(q32;q11) chromosomal translocations found in MM cells. Double color fluorescence in situ hybridization analyses pinpointed the breakpoints at the 20q11 locus in two MM cell lines within a length of at most 680 kb between the KIAA0823 and MAFB gene loci. Among the transcribed sequences in the vicinity of the breakpoints, an ectopic expression of the MAFB gene, which is located at 450 - 680 kb telomeric to one of the breakpoints and encodes a member of the MAF family basic region / leucine zipper transcription factor, was demonstrated to be associated with t(14;20). This finding, together with that of a previous study describing its transforming activity, suggests that the MAFB gene may be one of the targets deregulated by regulatory elements of the IgH gene as a result of t(14;20).
Hanamura
I
I
Second Department of Internal Medicine, Nagoya City University Medical School, 1 Kawasumi, Mizuho-chou, Mizuho-ku, Nagoya 467-8601, Japan.
Iida
S
S
Akano
Y
Y
Hayami
Y
Y
Kato
M
M
Miura
K
K
Harada
S
S
Banno
S
S
Wakita
A
A
Kiyoi
H
H
Naoe
T
T
Shimizu
S
S
Sonta
S I
SI
Nitta
M
M
Taniwaki
M
M
Ueda
R
R
D030161:Avian Proteins; D015152:Blotting, Northern; D018244:Chromosomes, Artificial, Yeast; D002883:Chromosomes, Human, Pair 14; D002890:Chromosomes, Human, Pair 20; D004268:DNA-Binding Proteins; D004742:Enhancer Elements, Genetic; D005803:Genes, Immunoglobulin / Q000235:genetics; D005819:Genetic Markers; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D051258:MafB Transcription Factor; D008957:Models, Genetic; D009101:Multiple Myeloma / Q000235:genetics* / Q000378:metabolism*; D015513:Oncogene Proteins / Q000096:biosynthesis*; D010641:Phenotype; D012333:RNA, Messenger / Q000378:metabolism; D016324:Sequence Tagged Sites; D015534:Trans-Activators / Q000096:biosynthesis*; D014157:Transcription Factors; D014178:Translocation, Genetic; D014407:Tumor Cells, Cultured
D030161:Avian Proteins; D004268:DNA-Binding Proteins; D005819:Genetic Markers; C490407:MAFB protein, human; D051258:MafB Transcription Factor; D015513:Oncogene Proteins; D012333:RNA, Messenger; D015534:Trans-Activators; D014157:Transcription Factors