title: [clpP2 gene encoding peptidase in cyanobacteria Synechocystis sp. PCC 6803 controls the sensitivity of cells to photoinhibition].
abstract: A homozygous insertion mutant with the inactivated clpP2 gene, which encodes the proteolytic subunit of ATP-dependent peptidase, was obtained in the unicellular cyanobacterium Synechocystis sp. PCC 6803. The mutant cannot grow under photoautotrophic conditions, but cells grown under heterotrophic conditions in a glucose-containing medium have active photosystems I and II (PS I and PS II). The loss of capacity for photoautotrophic growth is determined by a high sensitivity of mutant cells to the inactivating effect of light. Their incubation under light with an intensity above 10 microE m-2 s-1 inhibits cell growth in culture and causes degradation of photosynthetic pigments. It is proposed that the ClpP2 peptidase is involved in the protection of Synechocystis 6803 cells from photoinhibition.
authors: Biological Faculty, Moscow State University, Vorob'evy gory, Moscow, 119889 Russia.
authors: V B
authors: VB
authors: Panichkin
authors: V M
authors: VM
authors: Glazer
authors: V V
authors: VV
authors: Zinchenko
authors: A
authors: A
authors: Sokolenko
authors: R G
authors: RG
authors: Herrmann
authors: S V
authors: SV
authors: Shestakov
mesh_terms: D001426:Bacterial Proteins; D003470:Culture Media; D000458:Cyanobacteria / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D005947:Glucose; D008027:Light; D016254:Mutagenesis, Insertional; D009154:Mutation; D045322:Photosynthetic Reaction Center Complex Proteins / Q000378:metabolism; D010860:Pigments, Biological / Q000378:metabolism; D012697:Serine Endopeptidases / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D045322:Photosynthetic Reaction Center Complex Proteins; D010860:Pigments, Biological; C119658:ClpP2 protein, bacteria; D012697:Serine Endopeptidases; D005947:Glucose