Induction of interleukin-8 in human retinal pigment epithelial cells after denuding injury.
OBJECTIVE
To determine interleukin 8 (IL-8) and monocyte chemoattractant protein 1 (MCP-1) expression in response to mechanical injury in human retinal pigment epithelial (HRPE) cells.


METHODS
Enzyme linked immunosorbent assay (ELISA) was performed to determine IL-8 and MCP-1 secretion by HRPE cells after mechanical denudation. IL-8 and MCP-1 mRNA expression by HRPE cells was assessed using semiquantitative RT-PCR. The effects of immunosuppressive drugs, dexamethasone (DEX) and cyclosporin A (CSA), as well as immunosuppressive cytokines, interleukin 4 (IL-4), interleukin 10 (IL-10), and interleukin 13 (IL-13), on chemokine expression in HRPE cells after denuding injury were analysed.


RESULTS
Mechanical injury induced HRPE IL-8 mRNA and IL-8 secretion. Although MCP-1 mRNA was enhanced slightly after denuding injury, MCP-1 secretion was not increased. DEX and CSA inhibited HRPE chemokine expression after injury. IL-4 and IL-13 enhanced IL-8 and MCP-1 production by HRPE cells after injury while IL-10 had no effect.


CONCLUSIONS
These results suggest that IL-8 may be involved in retinal inflammatory responses to injury and that DEX and/or CSA treatment may help control the inflammatory components of retinal diseases such as proliferative vitreoretinopathy.
Yoshida
A
A
Department of Ophthalmology, University of Michigan, Ann Arbor, MI 48105, USA.
Elner
S G
SG
Bian
Z M
ZM
Elner
V M
VM
D000328:Adult; D000704:Analysis of Variance; D018932:Chemokine CCL2 / Q000032:analysis* / Q000235:genetics; D016572:Cyclosporine / Q000494:pharmacology; D003907:Dexamethasone / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D004847:Epithelial Cells / Q000276:immunology; D006801:Humans; D007150:Immunohistochemistry; D007166:Immunosuppressive Agents / Q000494:pharmacology; D016753:Interleukin-10 / Q000494:pharmacology; D018793:Interleukin-13 / Q000494:pharmacology; D015847:Interleukin-4 / Q000494:pharmacology; D016209:Interleukin-8 / Q000032:analysis* / Q000235:genetics; D008875:Middle Aged; D010857:Pigment Epithelium of Eye / Q000276:immunology* / Q000293:injuries*; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018630:Vitreoretinopathy, Proliferative / Q000188:drug therapy
D018932:Chemokine CCL2; D007166:Immunosuppressive Agents; D018793:Interleukin-13; D016209:Interleukin-8; D012333:RNA, Messenger; D016753:Interleukin-10; D015847:Interleukin-4; D003907:Dexamethasone; D016572:Cyclosporine