The NS2 protein generated by the parvovirus minute virus of mice is degraded by the proteasome in a manner independent of ubiquitin chain elongation or activation.
The NS2 protein generated by the parvovirus minute virus of mice is very labile, having a half-life during infection of approximately 90 min. The degradation of NS2 is blocked by proteasome inhibitors but is likely ubiquitin independent: NS2 does not form detectable higher molecular weight ubiquitin-containing conjugates, and NS2 degradation requires neither ubiquitin chain elongation nor intracellular ubiquitin activation. We have also identified a region in the carboxyl half of NS2 that is required for its proteasome-mediated degradation.
Miller
C L
CL
Department of Molecular Microbiology and Immunology, University of Missouri at Columbia School of Medicine, Columbia, Missouri 65212, USA.
Pintel
D J
DJ
D000590:Amino Acid Chloromethyl Ketones / Q000494:pharmacology; D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002460:Cell Line; D002874:Chromosome Mapping; D003546:Cysteine Endopeptidases / Q000378:metabolism*; D015853:Cysteine Proteinase Inhibitors / Q000494:pharmacology; D004789:Enzyme Activation; D007424:Intracellular Fluid / Q000378:metabolism; D051379:Mice; D008915:Minute Virus of Mice / Q000378:metabolism*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009097:Multienzyme Complexes / Q000037:antagonists & inhibitors / Q000378:metabolism*; D046988:Proteasome Endopeptidase Complex; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D014452:Ubiquitins / Q000378:metabolism*; D017361:Viral Nonstructural Proteins / Q000235:genetics / Q000378:metabolism*
D000590:Amino Acid Chloromethyl Ketones; D015853:Cysteine Proteinase Inhibitors; D009097:Multienzyme Complexes; C081367:NS1 protein, minute virus of mice; D011993:Recombinant Fusion Proteins; D014452:Ubiquitins; D017361:Viral Nonstructural Proteins; C096713:benzyloxycarbonylvalyl-alanyl-aspartyl fluoromethyl ketone; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex