Crystallization and preliminary X-ray crystallographic studies on 4-alpha-glucanotransferase from Thermotoga maritima.
Thermotoga maritima 4-alpha-glucanotransferase (GTase), a 52 kDa molecular-weight amylolytic enzyme, has been crystallized by the hanging-drop vapour-diffusion method using PEG monomethylether 5000 as a precipitating agent. A complete data set has been collected to 2.6 A resolution using cryocooling conditions and synchrotron radiation. The crystals belong to space group I222 or I2(1)2(1)2(1), with unit-cell parameters a = 92.6, b = 180.3, c = 199.2 A.
Roujeinikova
A
A
Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, The University of Sheffield, England.
Raasch
C
C
Sedelnikova
S
S
Liebl
W
W
Rice
D W
DW
D018360:Crystallography, X-Ray; D006004:Glycogen Debranching Enzyme System / Q000737:chemistry*; D011487:Protein Conformation; D020124:Thermotoga maritima / Q000201:enzymology*
D006004:Glycogen Debranching Enzyme System; C020569:4 alpha-glucanotransferase