title: The effect of sodium heparin on equine articular cartilage.
abstract: This study compared the effect of sodium heparin and gentamicin sulphate on equine articular cartilage (AC) explants in order to investigate the possible use of sodium heparin in the treatment of infectious arthritis. Six concentrations of sodium heparin and gentamicin sulphate were tested. The supernatant and explant digest were assayed for glycosaminoglycan (GAG) content with the dimethyl-methylene blue assay and the per cent loss of GAG was calculated. A significant (P< 0.001) increase in percentage GAG loss was noted for the sodium heparin groups when compared to the control, whilst no significant increase was found among the treatment groups (P =0.782). For gentamicin, no significant difference in percentage GAG loss was found between the control and three of the five treatment groups (P =0.667). The percentage GAG loss in the sodium heparin treated AC explants was greater than for any of the gentamicin-treated AC explants. It can be concluded that sodium heparin sulphate stimulates an increase in GAG release from equine articular cartilage explants, though no firm conclusions can be drawn on its use in treating equine infectious arthritis.
authors: Welsh Institute of Rural Studies, University of Wales Aberystwyth, Llanbadarn Fawr, Aberystwyth, Ceredigion, SY23 3AL, UK. helen75@liv.ac.uk
authors: H E
authors: HE
authors: McCarthy
authors: E R
authors: ER
authors: Singer
authors: M C
authors: MC
authors: Davies Morel
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology* / Q000627:therapeutic use; D000925:Anticoagulants / Q000494:pharmacology* / Q000627:therapeutic use; D001170:Arthritis, Infectious / Q000188:drug therapy / Q000662:veterinary; D002358:Cartilage, Articular / Q000187:drug effects* / Q000378:metabolism; D005839:Gentamicins / Q000494:pharmacology* / Q000627:therapeutic use; D006025:Glycosaminoglycans / Q000378:metabolism; D006493:Heparin / Q000494:pharmacology* / Q000627:therapeutic use; D006734:Horse Diseases / Q000188:drug therapy; D006736:Horses
chemical_list: D000900:Anti-Bacterial Agents; D000925:Anticoagulants; D005839:Gentamicins; D006025:Glycosaminoglycans; D006493:Heparin