title: Allergenic cross-reactivity between the nematode Anisakis simplex and the dust mites Acarus siro, Lepidoglyphus destructor, Tyrophagus putrescentiae, and Dermatophagoides pteronyssinus.
abstract: BACKGROUND
  The nematode Anisakis simplex is a common parasite on fish and other seafood. It is considered to be a food allergen and to induce IgE-mediated reactions. Allergenic cross-reactivity between A. simplex and other nematodes has been reported, as has cross-reactivity with arthropods: red mosquito larvae and German cockroach. We have here studied the allergenic relationship between A. simplex and four different dust-mite species.
  
  
  METHODS
  Serum samples collected from 69 farmers allergic to dust mites were analyzed for IgE to A. simplex by CAP FEIA. Allergenic cross-reactivity between A. simplex and dust mites was studied in two of the sera by CAP FEIA and immunoblotting inhibition.
  
  
  RESULTS
  We found that 14/69 farmers had detectable levels of IgE antibodies to A. simplex. The IgE response in CAP FEIA to A. simplex was inhibited to various degrees in the two studied sera by extracts of the dust mites Acarus siro, Lepidoglyphus destructor, Tyrophagus putrescentiae, and Dermatophagoides pteronyssinus. In the reverse inhibition experiment, extract of A. simplex inhibited the response in both sera to A. siro and T. putrescentiae, but not to L. destructor. The IgE binding to D. pteronyssinus was inhibited in one of the two sera. In blotting inhibition experiments, the IgE binding to several allergens in A. simplex was inhibited by each of the four mite extracts, especially by A. siro and T. putrescentiae, which completely inhibited the IgE binding to several allergens.
  
  
  CONCLUSIONS
  The results show allergenic cross-reactivity between several allergens in A. simplex and four dust-mite species. The clinical significance of this cross-reactivity remains to be evaluated.
authors: Department of Medicine, Unit of Clinical Immunology and Allergy, Karolinska Institutet and Hospital, Stockholm, Sweden.
authors: E
authors: E
authors: Johansson
authors: M
authors: M
authors: Aponno
authors: M
authors: M
authors: Lundberg
authors: M
authors: M
authors: van Hage-Hamsten
mesh_terms: D000818:Animals; D017163:Anisakis / Q000276:immunology*; D000906:Antibodies / Q000032:analysis / Q000276:immunology; D039741:Antigens, Dermatophagoides; D003429:Cross Reactions / Q000276:immunology*; D004391:Dust / Q000009:adverse effects*; D006023:Glycoproteins / Q000276:immunology*; D006801:Humans; D006967:Hypersensitivity / Q000209:etiology* / Q000276:immunology*; D015151:Immunoblotting; D007073:Immunoglobulin E / Q000276:immunology / Q000378:metabolism; D008222:Lymphokines / Q000276:immunology / Q000378:metabolism; D008925:Mites / Q000276:immunology*; D029584:Prostatic Secretory Proteins; D011852:Radioallergosorbent Test; D018709:Statistics, Nonparametric
chemical_list: D000906:Antibodies; D039741:Antigens, Dermatophagoides; D004391:Dust; D006023:Glycoproteins; D008222:Lymphokines; D029584:Prostatic Secretory Proteins; C043076:beta-microseminoprotein; C013059:immunoglobulin-binding factors; D007073:Immunoglobulin E