MALDI-ToF/MS as a diagnostic tool for the confirmation of sulfur mustard exposure.
The continual threat of chemical and biological warfare has prompted the need for unambiguous analytical methods for the confirmation of agent exposure. In this paper, we have investigated the use of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-ToF/MS) as a diagnostic tool for this purpose. Mass spectral studies of the interaction of sulfur mustard (bis-(2-chloroethyl) sulfide, HD) with hemoglobin and metallothioneine were conducted. In vitro experiments with purified proteins were performed, using both HD and chloroethylethyl sulfide (CEES), in an effort to determine the extent of alkylation and occurrence of HD cross-linking using the MALDI-ToF/MS technique. In a typical experiment, 50 ml of 5 mM HD in acetonitrile was added to an equal volume of 0.5 mM hemoglobin in deionized water followed by vortexing and incubation at room temperature. After 24 h, the samples were analyzed by MALDI-ToF/MS. Mass spectral results indicated the presence of at least two distinct alkylation adducts for both HD and CEES experiments. These results demonstrate that MALDI-ToF/MS is a useful analytical technique to investigate the interaction of HD with biomolecules and may be employed potentially as a diagnostic tool for the confirmation of exposure to chemical warfare agents.
Price
E O
EO
Pharmacology Division, Applied Pharmacology Branch, US Army Medical Research Institute of Chemical Defense, Aberdeen Proving Ground, MD 21010-5425, USA.
Smith
J R
JR
Clark
C R
CR
Schlager
J J
JJ
Shih
M L
ML
D000097:Acetonitriles; D000818:Animals; D003879:Dermatologic Agents / Q000506:poisoning*; D006454:Hemoglobins / Q000737:chemistry; D006801:Humans; D008668:Metallothionein / Q000737:chemistry; D009151:Mustard Gas / Q000031:analogs & derivatives / Q000506:poisoning*; D011041:Poisoning / Q000175:diagnosis; D011817:Rabbits; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D000097:Acetonitriles; D003879:Dermatologic Agents; D006454:Hemoglobins; C031278:2-chloroethyl ethyl sulfide; D008668:Metallothionein; D009151:Mustard Gas; C032159:acetonitrile