[Cloning and expression of a synthetic gene encoding magainin-melittin hybrid peptide in Escherichia coli and studies on its antibacterial activity].
A hybrid peptide gene was designed and synthesized. Its encoding peptide is constructed from residues 3-14 of magainin and residues 1-13 of melittin. The MA-E gene was cloned into plasmids pUC18 and pBV220. By DNA sequencing, the whole sequences of this gene is confirmed to be correct. The recombinant plasmid pBMA-E was expressed in E. coli DH5 alpha. A gene product band can be seen with Tricine-SDS-PAGE. The MA-E hybrid peptide was purified by immobilized metal affinity chromatography. Bioactivity assay was carried out in liquid turbidity method. The bactericide value to E. coli K12D31 is 0.182.
Huang
Y
Y
Department of Biotechnology, Jinan University, Guangzhou 510632, China.
Liu
F P
FP
Zhou
T H
TH
Zhu
J M
JM
D023181:Antimicrobial Cationic Peptides / Q000096:biosynthesis* / Q000235:genetics / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics*; D008555:Melitten / Q000096:biosynthesis* / Q000235:genetics / Q000494:pharmacology; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000494:pharmacology; D029867:Xenopus Proteins
D023181:Antimicrobial Cationic Peptides; D011993:Recombinant Fusion Proteins; D029867:Xenopus Proteins; C053300:magainin 1 peptide, Xenopus; D008555:Melitten