Enzymatic synthesis of two ginsenoside Re-beta-xylosides.
Two new beta-xylosyl derivatives of ginsenoside Re, 20(S)-protopanaxatriol 6-O-alpha-L-rhamnopyranosyl-(1 --> 2)-[beta-D-xylopyranosyl-(1 --> 4)]-beta-D-glucopyranosyl-20-O-beta-D-glucopyranoside and 20(S)-protopanaxatriol 6-O-alpha-L-rhamnopyranosyl-(1 --> 2)-[beta-D-xylopyranosyl-(1 --> 6)]-beta-D-glucopyranosyl-20-O-beta-D-glucopyranoside, were respectively synthesized from p-nitrophenyl beta-D-xylopyranoside and phenyl beta-D-xylopyranoside as donors and ginsenoside Re as the acceptor in 25% acetone and acetonitrile by a cellulase preparation from Trichoderma viride and a beta-galactosidase preparation from Aspergillus oryzae. The latter enzyme preparation also catalyzed the hydrolysis of ginsenoside Re to the minor saponin, ginsenoside Rg2.
Ko
S R
SR
Korea Ginseng and Tobacco Research Institute, Yusong-ku, Taejon.
Suzuki
Y
Y
Kim
Y H
YH
Choi
K J
KJ
D001236:Aspergillus oryzae / Q000201:enzymology; D002384:Catalysis; D002480:Cellulase / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D036145:Ginsenosides; D006027:Glycosides / Q000138:chemical synthesis*; D006868:Hydrolysis; D015394:Molecular Structure; D012503:Saponins / Q000096:biosynthesis* / Q000737:chemistry; D013057:Spectrum Analysis; D014242:Trichoderma / Q000201:enzymology; D001616:beta-Galactosidase / Q000378:metabolism*
D036145:Ginsenosides; D006027:Glycosides; D012503:Saponins; C010807:xylosides; C049864:ginsenoside Re; D001616:beta-Galactosidase; D002480:Cellulase