Some features of intestinal absorption of intact fibrinolytic enzyme III-1 from Lumbricus rubellus.
In order to investigate whether earthworm fibrinolytic enzyme III-1 (EFE-III-1) isolated from Lumbricus rubellus is capable of transporting into blood through intestinal epithelium and keeping its biological function in circulation, we have raised an antibody against EFE-III-1. The immunological results showed that 10-15% of intact EFE-III-1 was absorbed by the intestinal epithelium with the incubation chamber method [Vilhardt and Lundin, Acta Physiol. Scand. 126 (1986) 601-607]. The enzyme could be detected in the intestinal epithelial cells by immunohistochemistry. Furthermore, immunoreactive intact EFE-III-1 was found in serum or plasma after intraperitoneal injection of rats. Approx. 10% of the full-size enzyme could transport through the intestinal epithelium. The maximum remaining activity in blood could be assayed around 60 min after the intraperitoneal injection.
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Laboratory of Visual Information Processing, Institute of Biophysics, Baiao Pharmaceuticals Beijing C.L., Chinese Academy of Sciences, 15 Da Tun Rd, Chaoyang District, 100101, Beijing, PR China.
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Li
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Fan
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Wu
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D000595:Amino Acid Sequence; D000818:Animals; D001692:Biological Transport; D002463:Cell Membrane Permeability; D004386:Duodenum / Q000187:drug effects / Q000378:metabolism; D010450:Endopeptidases / Q000097:blood / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism; D005343:Fibrinolytic Agents / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D005629:Frozen Sections; D015151:Immunoblotting; D007150:Immunohistochemistry; D007274:Injections, Intraperitoneal; D007408:Intestinal Absorption; D008969:Molecular Sequence Data; D009835:Oligochaeta / Q000201:enzymology*; D051381:Rats
D005343:Fibrinolytic Agents; D010450:Endopeptidases; C083421:lumbrokinase