title: Novel signal amplification technology with applications in DNA and protein detection systems.
abstract: A non-enzymatic approach to signal amplification has practical advantages over conventional target amplification methods. We have designed a simple, cost-efficient signal amplification system that can be used to enhance the detection of nucleic acids or protein. The signal amplification process requires initial capture of analyte by a specific probe, which, depending on the analyte, can be an oligomer or an antibody. Once the analyte is captured, amplification moieties are applied to significantly enhance the sensitivity of analyte detection. Nucleic acid amplification is typically greater than 1000-fold, increasing the sensitivity of target detection to less than 1 amol/100 microL. This amplification strategy presents a very flexible system with components that are easily altered to accommodate diverse assay requirements.
authors: Tm Bioscience Corporation, 439 University Avenue, Suite 1100, Toronto, ON M5G 1Y8, Canada. conniem@tmbioscience.com
authors: C M
authors: CM
authors: Lisle
authors: S
authors: S
authors: Bortolin
authors: A S
authors: AS
authors: Benight
authors: R A
authors: RA
authors: Janeczko
authors: R L
authors: RL
authors: Zastawny
mesh_terms: D000818:Animals; D019921:Biotinylation; D004247:DNA / Q000032:analysis*; D016650:Fluorescein-5-isothiocyanate / Q000032:analysis*; D005456:Fluorescent Dyes / Q000032:analysis*; D005470:Fluorometry / Q000379:methods*; D005838:Genotype; D006801:Humans; D008863:Microspheres; D009693:Nucleic Acid Hybridization; D011061:Poly A / Q000032:analysis* / Q000737:chemistry; D011071:Poly T / Q000032:analysis* / Q000737:chemistry; D011506:Proteins / Q000032:analysis*; D012680:Sensitivity and Specificity; D019809:Streptavidin / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D011506:Proteins; D011061:Poly A; D011071:Poly T; C015465:poly(dA); D004247:DNA; D019809:Streptavidin; D016650:Fluorescein-5-isothiocyanate