Uptake of a cholesterol-rich emulsion by neoplastic ovarian tissues.
OBJECTIVE
Previously, it was shown that a lipidic emulsion (LDE) composed of phospholipids and cholesterol esters which binds to low-density lipoprotein (LDL) receptors may concentrate in acute myeloid leukemia cells. In this study, we aimed to verify whether LDE also has the ability to concentrate in malignant ovarian cancer after being injected into the blood circulation of the patients.


METHODS
Three groups of women scheduled for surgery were included in the survey: 13 bearing malignant tumors, 9 with benign ovarian tumors, and 13 without ovarian tumor who were scheduled to undergo oophorectomy due to malignant disease of the uterine cervix or endometrium. On the day prior to surgery they were injected with LDE labeled with [(14)C]cholesteryl oleate. Specimens of tumors and normal ovaries excised during surgery were lipid extracted and analyzed for radioactivity counting. Results were expressed in radioactive count (cpm) per gram of tissue.


RESULTS
The mean of the uptakes of the emulsion radioactivity by the malignant tumors was roughly eightfold greater when compared with that of the contralateral normal ovaries (2261 +/- 1444 and 275 +/- 137 cpm/g, respectively, P < 0.012), benign tumors, and normal ovaries of the patients without ovarian tumors.


CONCLUSIONS
LDE has the ability to concentrate in malignant ovarian tumor tissue. Therefore, it can be used as a vehicle to direct cytotoxic drugs against malignant ovarian tumors, thus diminishing the side effects of chemotherapy.
Ades
A
A
Department of Obstetrics and Gynecology, University of São Paulo, São Paulo, Brazil.
Carvalho
J P
JP
Graziani
S R
SR
Amancio
R F
RF
Souen
J S
JS
Pinotti
J A
JA
Maranhão
R C
RC
D000328:Adult; D000368:Aged; D002788:Cholesterol Esters / Q000737:chemistry / Q000493:pharmacokinetics*; D008078:Cholesterol, LDL / Q000493:pharmacokinetics; D004655:Emulsions; D005260:Female; D006801:Humans; D008875:Middle Aged; D010051:Ovarian Neoplasms / Q000000981:diagnostic imaging / Q000378:metabolism* / Q000601:surgery; D011877:Radionuclide Imaging; D011973:Receptors, LDL / Q000378:metabolism
D002788:Cholesterol Esters; D008078:Cholesterol, LDL; D004655:Emulsions; D011973:Receptors, LDL; C014409:cholesteryl oleate