Expression of bovine leukemia virus protein p24 in Escherichia coli and its use in the immunoblotting assay.
The gag gene encoded protein, p24 of bovine leukemia virus (BLV), was cloned and expressed as thioredoxin-6xHis-p24 protein in Escherichia coli. The bacterial cells carrying plasmid pT7THis-p24 expressed the protein of 38 kDa that was detected by immunoblotting analysis using anti-p24 monoclonal antibodies and sera from BLV infected cattle and sheep. The purified p24 fusion protein was shown to be sensitive and specific for detection of BLV antibodies in the infected cattle.
Bicka
L
L
Department of Biochemistry, National Veterinary Research Institute, Puławy, Poland.
Kuźmak
J
J
Kozaczyńska
B
B
Plucienniczak
A
A
Skorupska
A
A
D000818:Animals; D015153:Blotting, Western; D002417:Cattle; D004926:Escherichia coli / Q000378:metabolism*; D015683:Gene Products, gag / Q000378:metabolism; D015151:Immunoblotting / Q000379:methods; D001909:Leukemia Virus, Bovine / Q000276:immunology; D010957:Plasmids / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012756:Sheep; D013879:Thioredoxins / Q000378:metabolism; D014758:Viral Core Proteins / Q000096:biosynthesis* / Q000737:chemistry*
D015683:Gene Products, gag; D011993:Recombinant Fusion Proteins; D014758:Viral Core Proteins; C089189:core protein p24, bovine leukemia virus; D013879:Thioredoxins