Oligoclonal expansion of CD4(+)CD28(-) T lymphocytes in recipients of allogeneic hematopoietic cell grafts and identification of the same T cell clones within both CD4(+)CD28(+) and CD4(+)CD28(-) T cell subsets.
Recipients of allogeneic bone marrow grafts have clonally expanded CD8(+)CD28(-) T lymphocytes during the early period after transplantation, which leads to skewing of T cell receptor (TCR) repertoires. Here, we have addressed the question of whether clonal expansion of CD28(-) T cells is also observed in CD4(+) T lymphocytes after human allogeneic hematopoietic cell transplantation. We found that the fraction of T cells lacking CD28 expression in the CD4(+) subset was increased after transplantation, and expanded CD4(+)CD28(-) T lymphocytes carrying certain TCRBV subfamilies showed limited TCR diversity. In order to further study the ontogeny of CD4(+)CD28(-) T cells, we analyzed the complementarity-determining region 3 (CDR3) of the TCR-beta chain of CD4(+)CD28(+) and CD4(+)CD28(-) cells. We identified the same T cell clones within both CD4(+)CD28(-) and CD4(+)CD28(+) T cell subsets. These results suggest that both subsets are phenotypic variants of the same T cell lineage.
Hirokawa
M
M
Department of Internal Medicine III, Akita University School of Medicine, Akita, Japan.
Horiuchi
T
T
Kawabata
Y
Y
Kitabayashi
A
A
Saitoh
H
H
Ichikawa
Y
Y
Matsutani
T
T
Yoshioka
T
T
Tsuruta
Y
Y
Suzuki
R
R
Miura
A
A
D000595:Amino Acid Sequence; D018106:CD28 Antigens / Q000032:analysis*; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology*; D002455:Cell Division; D019070:Cell Lineage; D002999:Clone Cells; D022801:Complementarity Determining Regions / Q000737:chemistry / Q000235:genetics; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D016693:Receptors, Antigen, T-Cell, alpha-beta; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D014184:Transplantation, Homologous
D018106:CD28 Antigens; D022801:Complementarity Determining Regions; D016693:Receptors, Antigen, T-Cell, alpha-beta