Unusual adaptive, cross protection responses and growth phase resistance against peroxide killing in a bacterial shrimp pathogen, Vibrio harveyi.
Oxidant induced protection against peroxide killing was investigated in a prawn bacterial pathogen, Vibrio harveyi. Exposure to 250 microM H(2)O(2) induced adaptive protection against subsequent exposure to killing concentrations of H(2)O(2). In addition, 200 microM t-butyl hydroperoxide (tBOOH) induced cross protection to H(2)O(2) killing. On the other hand, peroxide pretreatment did not induce protection against tBOOH killing. Peroxide induced adaptive and cross protection responses required new protein synthesis and were abolished by addition of a protein synthesis inhibitor. Pretreatments of V. harveyi with 250 microM H(2)O(2) and 200 microM tBOOH induced an increase in peroxide scavenging enzymes, catalase and alkyl hydroperoxide reductase subunit C. In addition, stationary phase cells of V. harveyi were more resistant to H(2)O(2) and iodoacetamide killing but highly susceptible to tBOOH killing compared to exponential phase cells. Many aspects of the oxidative stress response of V. harveyi are different from those of other bacteria and these factors may be important for bacterial survival in the environment and during interactions with host shrimp.
Vattanaviboon
P
P
Laboratory of Biotechnology, Chulabhorn Research Institute, Lak Si, Bangkok, Thailand. paiboon@tubtim.cri.or.th
Mongkolsuk
S
S
D000220:Adaptation, Biological; D000818:Animals; D001426:Bacterial Proteins; D002374:Catalase / Q000096:biosynthesis; D033364:Decapoda / Q000382:microbiology*; D004351:Drug Resistance; D004790:Enzyme Induction; D006861:Hydrogen Peroxide / Q000633:toxicity*; D016877:Oxidants / Q000633:toxicity; D018384:Oxidative Stress; D010544:Peroxidases / Q000096:biosynthesis; D054464:Peroxiredoxins; D014733:Vibrio / Q000187:drug effects*; D014735:Vibrio Infections / Q000662:veterinary
D001426:Bacterial Proteins; D016877:Oxidants; D006861:Hydrogen Peroxide; D010544:Peroxidases; D054464:Peroxiredoxins; D002374:Catalase; C090103:KATA protein, Bacillus subtilis