The flavonoids, quercetin and isorhamnetin 3-O-acylglucosides diminish neutrophil oxidative metabolism and lipid peroxidation.
Two natural flavonoids, quercetin and isorhamnetin 3-O-acylglucosides, were examined for their inhibitory influence on the in vitro production and release of reactive oxygen species in polymorphonuclear neutrophils (PMNs). The generation of superoxide radical, hydrogen peroxide and hypochlorous acid were measured by, respectively, cytochrome c reduction, dichlorofluorescin oxidation and taurine chlorination. Membrane lipid oxidation was studied by the thiobarbituric acid method in mouse spleen microsomes. The addition of flavonoids at the concentration range 1-100 microM inhibited PMNs oxidative metabolism and lipid peroxidation in a dose-dependent manner. The results suggest that these flavonoids suppress the oxidative burst of PMNs and protect membranes against lipid peroxidation.
Zielińska
M
M
Department of Pharmaceutical Biochemistry, Karol Marcinkowski University of Medical Sciences, Poznań, Poland. mzielin@mail.usoms.poznan.pl
Kostrzewa
A
A
Ignatowicz
E
E
Budzianowski
J
J
D000328:Adult; D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D002713:Chlorine / Q000378:metabolism; D005260:Female; D005419:Flavonoids / Q000494:pharmacology*; D044948:Flavonols; D005434:Flow Cytometry; D005452:Fluoresceins / Q000378:metabolism; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects*; D051379:Mice; D008861:Microsomes / Q000378:metabolism; D008956:Models, Chemical; D009504:Neutrophils / Q000187:drug effects*; D010100:Oxygen / Q000378:metabolism*; D011485:Protein Binding; D011794:Quercetin / Q000031:analogs & derivatives* / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013654:Taurine / Q000378:metabolism
D005419:Flavonoids; D044948:Flavonols; D005452:Fluoresceins; D017382:Reactive Oxygen Species; D013654:Taurine; D002713:Chlorine; C037631:2',7'-dichlorofluorescein; D011794:Quercetin; C432511:isorhamnetin 3-O-glucoside; D010100:Oxygen