Probing the mechanism of a cyanobacterial Delta9 fatty acid desaturase from Spirulina platensis C1 (Arthrospira sp. PCC 9438).
The initial and rate determining step in the mechanism of fatty acid desaturases has been proposed to be breakage of one of the C&z.sbnd;H bonds at the site of the incipient double bond. This has been investigated and supported for a number of eukaryotic fatty acid desaturases through the use of kinetic isotope effect experiments with deuterated substrates. In order to probe the reaction catalyzed by the cyanobacterial Delta9 desaturase and compare it to the eukaryotic desaturases, the desC gene of Spirulina platensis, strain C1 (Arthrospira sp. PCC 9438) was expressed in a desaturase mutant of baker's yeast. Kinetic isotope effects were performed by culturing yeast transformants with deuterated thia-substituted stearic acids. A large kinetic isotope effect was found for the 9 position, in qualitative agreement with results from eukaryotic desaturases.
Meesapyodsuk
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National Research Council of Canada, Plant Biotechnology Institute, 110 Gymnasium Place, Saskatchewan, S7N 0W9, Saskatoon, Canada.
Reed
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Cheevadhanarak
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Deshnium
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Covello
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D000818:Animals; D000458:Cyanobacteria / Q000201:enzymology; D007700:Kinetics; D009154:Mutation; D010100:Oxygen / Q000378:metabolism; D011485:Protein Binding; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D013230:Stearoyl-CoA Desaturase / Q000737:chemistry*; D013997:Time Factors
D013230:Stearoyl-CoA Desaturase; D010100:Oxygen