Expression profiles of low molecular mass heat shock proteins by Mycobacterium avium and Mycobacterium intracellulare.
Closely related non-tuberculous mycobacterial species, Mycobacterium avium and Mycobacterium intracellulare, were compared for the profiles of their production of low molecular mass heat shock proteins at 45 degrees C, by performing polyacrylamide gel electrophoresis analysis of bacterial cell lysate proteins. All of the M. intracellulare but not M. avium strains potently increased the production of the 18-kDa heat shock protein, when cultured at 45 degrees C. Half of the M. intracellulare strains with high sensitivity to 45 degrees C produced not only the 18-kDa heat shock protein but also the 16-kDa heat shock protein at 45 degrees C. These findings indicate that M. avium and M. intracellulare differentially respond to 45 degrees C heat shock in terms of the production of low molecular mass heat shock proteins.
Tomioka
H
H
Department of Microbiology and Immunology, Shimane Medical University, Izumo, 693-8501, Shimane, Japan. tomioka@shimane-med.ac.jp
Shimizu
T
T
Kajitani
H
H
Sato
K
K
Sano
C
C
D006360:Heat-Shock Proteins / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D008970:Molecular Weight; D009162:Mycobacterium avium / Q000302:isolation & purification / Q000378:metabolism*; D015269:Mycobacterium avium Complex / Q000302:isolation & purification / Q000378:metabolism*; D020539:Sequence Analysis, Protein
D006360:Heat-Shock Proteins