The RNA-binding specificity of the mouse Dazl protein.
DAZ is an RNA-binding protein encoded by a region on the Y chromosome implicated in infertility, and DAZ-like (Dazl) proteins are master regulators of germ line gene expression in all animals. In mice Dazl is only expressed in germ cells and is necessary for meiosis. A dual approach was taken to understand the RNA-binding specificity of the Dazl protein: (i) traditional SELEX and (ii) a novel tri-hybrid screen. Both approaches led to the same conclusion, namely that Dazl binds oligo(U) stretches interspersed by G or C residues. In a directed tri-hybrid assay the strongest interaction was with the consensus (GUn)n. This motif is found in the 5' UTR of CDC25C whose homologue is thought to be the target of Boule, the Dazl homologue in flies. CDC25C 5' UTR also interacted specifically with Dazl in vitro. The tri-hybrid screen retrieved UTRs of known genes that may be physiological substrates of Dazl.
Venables
J P
JP
Medical Research Council Human Genetics Unit, Western General Hospital, Crewe Road, Edinburgh EH4 2XU, UK. j.venables@ncl.ac.uk
Ruggiu
M
M
Cooke
H J
HJ
D020121:5' Untranslated Regions / Q000235:genetics*; D000818:Animals; D001665:Binding Sites; D018797:Cell Cycle Proteins / Q000235:genetics*; D016384:Consensus Sequence / Q000235:genetics; D005786:Gene Expression Regulation; D051379:Mice; D011072:Poly U / Q000235:genetics; D011485:Protein Binding; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics; D013379:Substrate Specificity; D020798:Two-Hybrid System Techniques; D020687:cdc25 Phosphatases / Q000235:genetics*
D020121:5' Untranslated Regions; D018797:Cell Cycle Proteins; C101358:DAZL protein, human; D011506:Proteins; D016601:RNA-Binding Proteins; D011993:Recombinant Fusion Proteins; D011072:Poly U; C517009:CDC25C protein, human; C517010:Cdc25c protein, mouse; D020687:cdc25 Phosphatases