Determination of free D-aspartic acid, D-serine and D-alanine in the brain of mutant mice lacking D-amino acid oxidase activity.
A simple and precise method for the simultaneous determination of free D-aspartic acid, D-serine and D-alanine in mouse brain tissues was established, using a reversed-phase HPLC system with widely used pre-column derivatizing reagents, o-phthaldialdehyde and N-t-butyloxycarbonyl-L-cysteine. With the present method, the contents of these three D-amino acids in hippocampus, hypothalamus, pituitary gland, pineal gland and medulla oblongata as well as cerebrum and cerebellum of mutant mice lacking D-amino-acid oxidase activity were determined and compared with those obtained for control mice. In both mice, extremely high contents of D-serine were observed in forebrain (100-400 nmol/g wet tissue), and the contents were small in pituitary and pineal glands. While, D-serine contents in cerebellum and medulla oblongata of mutant mice were about ten times higher than those in control mice. In contrast, D-alanine contents in mutant mice were higher than those in control mice in all brain regions and serum.
Morikawa
A
A
Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan.
Hamase
K
K
Inoue
T
T
Konno
R
R
Niwa
A
A
Zaitsu
K
K
D000409:Alanine / Q000032:analysis*; D000818:Animals; D001224:Aspartic Acid / Q000032:analysis*; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid; D003605:D-Amino-Acid Oxidase / Q000235:genetics / Q000378:metabolism*; D008297:Male; D051379:Mice; D008817:Mice, Mutant Strains; D012694:Serine / Q000032:analysis*
D001224:Aspartic Acid; D012694:Serine; D003605:D-Amino-Acid Oxidase; D000409:Alanine