title: Complete development and long-term maintenance of Cryptosporidium parvum human and cattle genotypes in cell culture.
abstract: This study describes the complete development (from sporozoites to sporulated oocysts) of Cryptosporidium parvum (human and cattle genotypes) in the HCT-8 cell line. Furthermore, for the first time the complete life cycle was perpetuated in vitro for up to 25 days by subculturing. The long-term maintenance of the developmental cycle of the parasite in vitro appeared to be due to the initiation of the auto-reinfection cycle of C. parvum. This auto-reinfection is characterised by the production and excystation of new invasive sporozoites from thin-walled oocysts, with subsequent maintenance of the complete life cycle in vitro. In addition, thin-walled oocysts of the cattle genotype were infective for ARC/Swiss mice but similar oocysts of the human genotype were not. This culture system will provide a model for propagation of the complete life cycle of C. parvum in vitro.
authors: Division of Veterinary and Biomedical Sciences, Murdoch University, South Street, W.A. 6150, Murdoch, Australia.
authors: N S
authors: NS
authors: Hijjawi
authors: B P
authors: BP
authors: Meloni
authors: U M
authors: UM
authors: Morgan
authors: R C
authors: RC
authors: Thompson
mesh_terms: D000818:Animals; D001681:Biological Assay; D002417:Cattle; D018929:Cell Culture Techniques; D016785:Cryptosporidium parvum / Q000166:cytology / Q000235:genetics / Q000254:growth & development*; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D005838:Genotype; D006801:Humans; D051379:Mice; D008857:Microscopy, Interference; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
chemical_list: D016054:DNA, Protozoan; D004275:DNA, Ribosomal