title: Toxic-metabolite-producing bacteria and fungus in an indoor environment.
abstract: Toxic-metabolite-emitting microbes were isolated from the indoor environment of a building where the occupant was suffering serious building-related ill-health symptoms. Toxic substances soluble in methanol and inhibitory to spermatozoa at <10 microg (dry weight) ml(-1) were found from six bacterial isolates and one fungus. The substances from isolates of Bacillus simplex and from isolates belonging to the actinobacterial genera Streptomyces and Nocardiopsis were mitochondriotoxic. These substances dissipated the mitochondrial membrane potential (Deltapsi) of boar spermatozoa. The substances from the Streptomyces isolates also swelled the mitochondria. The substances from isolates of Trichoderma harzianum Rifai and Bacillus pumilus damaged the cell membrane barrier function of sperm cells.
authors: Division of Microbiology, Department of Applied Chemistry and Microbiology, University of Helsinki, FIN-00014 University of Helsinki, Finland. joanna.peltola@helsinki.fi
authors: J
authors: J
authors: Peltola
authors: M A
authors: MA
authors: Andersson
authors: T
authors: T
authors: Haahtela
authors: H
authors: H
authors: Mussalo-Rauhamaa
authors: F A
authors: FA
authors: Rainey
authors: R M
authors: RM
authors: Kroppenstedt
authors: R A
authors: RA
authors: Samson
authors: M S
authors: MS
authors: Salkinoja-Salonen
mesh_terms: D000391:Air Microbiology; D016902:Air Pollution, Indoor; D000818:Animals; D001427:Bacterial Toxins / Q000633:toxicity*; D020459:Genes, rRNA; D006094:Gram-Positive Bacteria / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism; D006798:Housing; D006801:Humans; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D008969:Molecular Sequence Data; D009183:Mycotoxins / Q000633:toxicity*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013094:Spermatozoa / Q000187:drug effects / Q000502:physiology; D014242:Trichoderma / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D001427:Bacterial Toxins; D009183:Mycotoxins; D012336:RNA, Ribosomal, 16S