Seminal plasma and IVF potential. Biochemical constituents of seminal plasma of males from in vitro fertilization couples.
OBJECTIVE
Many components of seminal plasma play a role in sperm motility by serving as energy sources. Human seminal plasma contains over 30 proteins, including forward motility proteins, antifertility proteins, and coagulation/liquefaction proteins. This study was designed to determine any correlation between motility or fertilization rates and concentrations of fructose, lactic acid, citric acid, carnitine, and protein in human seminal plasma.


METHODS
Fertilization rates were determined by in vitro methods. Fructose, lactic acid, citric acid, and carnitine concentrations were ascertained using high performance liquid chromatography. Protein concentration was determined by Bradford assay.


RESULTS
Protein concentrations were significantly different as a function of sperm motility levels. Other constituents of human seminal plasma showed an overall correlation, though not significant. No constituent exhibited significant differences as a function of fertility levels.


CONCLUSIONS
Protein concentration was significantly lower for samples with high motility. No significant differences between fertility levels and constituents measured were found.
Lay
M F
MF
Animal and Veterinary Sciences Department, Clemson University Clemson, South Carolina, USA.
Richardson
M E
ME
Boone
W R
WR
Bodine
A B
AB
Thurston
R J
RJ
D000328:Adult; D002331:Carnitine / Q000032:analysis; D019343:Citric Acid / Q000032:analysis; D005260:Female; D005307:Fertilization in Vitro; D005632:Fructose / Q000032:analysis; D006801:Humans; D019344:Lactic Acid / Q000032:analysis; D008297:Male; D011247:Pregnancy; D011506:Proteins / Q000032:analysis; D012661:Semen / Q000737:chemistry* / Q000502:physiology; D013081:Sperm Motility / Q000502:physiology*; D018709:Statistics, Nonparametric
D011506:Proteins; D019343:Citric Acid; D005632:Fructose; D019344:Lactic Acid; D002331:Carnitine