Unusual electrophoretic patterns for phosphoglucomutase and fumarase in a population of Lecithochirium rufoviride (Trematoda: Hemiuridae), a parasite of Conger conger.
Electrophoretic analyses of phosphoglucomutase (PGM) and fumarase (FH) in a population of Lecithochirium rufoviride parasitizing Conger conger, revealed 2 independent activity zones for each enzyme on starch gel electrophoresis. However, some individuals exhibited only 1 activity zone for 1 or both enzymes. The banding patterns observed strongly suggest that (1) PGM is coded by 2 polymorphic loci, Pgm-1 (expressed in all individuals) with allelic frequencies not significantly different from those expected under Hardy-Weinberg equilibrium, and Pgm-2 (expressed in a subset of individuals); and (2) FH is coded by 2 loci, Fh-2 (monomorphic and expressed in all individuals) and Fh-1 (expressed in a subset of individuals). A high degree of concordance (88.75%) was observed between the expression and nonexpression of Pgm-2 and Fh-1. The most likely explanations for these findings are either variation in enzyme expression with developmental stage or the presence of null alleles at high frequencies in the population.
Vilas
R
R
Departamento de Microbiología y Parasitología, Facultad de Farmacia, Universidad de Santiago de Compostela, Spain.
Paniagua
E
E
Sanmartín
M L
ML
Santamarina
M T
MT
Ubeira
F M
FM
D000818:Animals; D004524:Eels / Q000469:parasitology*; D004592:Electrophoresis, Starch Gel / Q000662:veterinary; D005393:Fish Diseases / Q000469:parasitology*; D005649:Fumarate Hydratase / Q000032:analysis* / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic; D005787:Gene Frequency; D010733:Phosphoglucomutase / Q000032:analysis* / Q000235:genetics; D014200:Trematoda / Q000201:enzymology* / Q000235:genetics; D014201:Trematode Infections / Q000469:parasitology / Q000662:veterinary*
D005649:Fumarate Hydratase; D010733:Phosphoglucomutase