Development of a simple whole blood panel test for detection of human heart-type fatty acid-binding protein.
OBJECTIVE
For the diagnosis of acute myocardial infarction (AMI), we have developed a rapid and simple whole blood panel test for the detection of human heart-type fatty acid-binding protein (H-FABP) using one-step immunochromatography.


RESULTS
We have developed a whole blood panel test for rapid detection of human H-FABP using a one-step immunochromatography technique. The result of this panel test was not affected by the other contents of the blood such as bilirubin, hemoglobin and others. Furthermore, no cross-reactivity of the antibodies was found with other cardiac markers or other tissue-type FABPs. The result of this panel test was similar to the diagnostic cut-off value, 6.2 ng of H-FABP per mL of serum which was evaluated by the enzyme-linked immunosorbent assay (ELISA).


CONCLUSIONS
We have developed a simple one-step immunochromatography technique to detect H-FABP in whole blood sample. Further studies are required to identify the value of this point-of-care testing (POCT) as a diagnostic marker for AMI.
Watanabe
T
T
Institute for Medical Research, Wakunaga Pharmaceutical Co., Ltd., 1624 Shimokotachi, Kodacho, Takatagun, Hiroshima 739-1195, Japan.
Ohkubo
Y
Y
Matsuoka
H
H
Kimura
H
H
Sakai
Y
Y
Ohkaru
Y
Y
Tanaka
T
T
Kitaura
Y
Y
D000911:Antibodies, Monoclonal / Q000378:metabolism; D002352:Carrier Proteins / Q000097:blood*; D002624:Chemistry, Clinical / Q000379:methods*; D002845:Chromatography / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D017979:Gold Colloid / Q000737:chemistry; D006801:Humans; D009203:Myocardial Infarction / Q000175:diagnosis; D009363:Neoplasm Proteins; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013997:Time Factors; D025521:Tumor Suppressor Proteins
D000911:Antibodies, Monoclonal; D002352:Carrier Proteins; C107608:FABP7 protein, human; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D017979:Gold Colloid; D009363:Neoplasm Proteins; D025521:Tumor Suppressor Proteins