Meiotic segregation analysis in a t(4;8) carrier: comparison of FISH methods on sperm chromosome metaphases and interphase sperm nuclei.
Meiotic segregation of a t(4;8)(q28;p23) translocation carrier was determined by two different methods to compare the final results. A total of 352 sperm chromosome complements, obtained after human-hamster in vitro fertilisation, were analysed by whole chromosome painting, and 6590 sperm heads were studied by fluorescence in situ hybridisation (FISH). Frequencies of alternate, adjacent I, adjacent II and 3 : 1 segregations were, for sperm chromosomes, 35.5, 33.2, 19.9 and 11.3% respectively. For sperm head analysis, results were 30.5, 28.5, 20.5 and 19.5% respectively. There were no statistically significant differences between the two methods with respect to the observed frequencies of sperm with balanced and unbalanced chromosome constitutions. Among unbalanced gametes, the methods differed only in the frequency of 3 : 1 segregation (chi(2), P<0.0001). Different factors that could explain this result are discussed. To determine possible interchromosomal effects, multicolour FISH was used on sperm heads. Disomy rates of sex and 18 chromosomes were higher in the translocation carrier than in the control. The differences observed were statistically significant (P<0.0001 for chromosomes X and 18, and P=0.0091 for chromosome Y).
Oliver-Bonet
M
M
Unitat de Biologia, Facultat de Medicina, Departament de Biologia Cel.lular, Fisiologia i d'Immunologia, Universitat Autònoma de Barcelona, Spain. moliver@servet.uab.es
Navarro
J
J
Codina-Pascual
M
M
Carrera
M
M
Egozcue
J
J
Benet
J
J
D000328:Adult; D000818:Animals; D002467:Cell Nucleus / Q000648:ultrastructure*; D020223:Chromosome Painting; D002894:Chromosomes, Human, Pair 4; D002898:Chromosomes, Human, Pair 8; D006224:Cricetinae; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D007399:Interphase; D008297:Male; D008540:Meiosis / Q000235:genetics*; D008677:Metaphase; D013094:Spermatozoa / Q000502:physiology / Q000648:ultrastructure*; D014178:Translocation, Genetic