A novel Coptis japonica multidrug-resistant protein preferentially expressed in the alkaloid-accumulating rhizome.
A full-length cDNA, Cjmdr1, which belongs to the multidrug-resistant (mdr) gene family, was isolated by nested RT-PCR from alkaloid-producing cultured cells of Coptis japonica. The cDNA is 4192 nucleotides long and has an ORF of 1289 amino acids. Northern analysis of the intact plant showed a clear preference in its expression in the rhizome, where alkaloids are highly accumulated compared to other organs.
Yazaki
K
K
Laboratory of Molecular and Cellular Biology of Totipotency, Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University, Kitashirakawa, Kyoto 606-8502, Japan.
Shitan
N
N
Takamatsu
H
H
Ueda
K
K
Sato
F
F
D000470:Alkaloids / Q000378:metabolism*; D000595:Amino Acid Sequence; D001483:Base Sequence; D017931:DNA Primers; D018076:DNA, Complementary; D019684:Magnoliopsida / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid
D000470:Alkaloids; C432805:CJMDR1 protein, Coptis japonica; D017931:DNA Primers; D018076:DNA, Complementary; D010940:Plant Proteins