Dual control of hydrogen cyanide biosynthesis by the global activator GacA in Pseudomonas aeruginosa PAO1.
The global response regulator GacA of Pseudomonas aeruginosa PAO1 positively controls the production of the quorum sensing signal molecule N-butanoyl-homoserine-lactone (C4-HSL) and hence the synthesis of several C4-HSL-dependent virulence factors, including hydrogen cyanide (HCN). This study presents evidence that GacA positively influences the transcription of the rhlI gene, specifying C4-HSL synthase, explaining the quorum sensing-dependent transcriptional control of the HCN biosynthetic genes (hcnABC). In addition, GacA was found to modulate hcn gene expression positively at a post-transcriptional level involving the hcnA ribosome-binding site. Thus, the activating effect of GacA on cyanogenesis results from both transcriptional and post-transcriptional mechanisms.
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Laboratoire de Biologie Microbienne, Université de Lausanne, CH-1015 Lausanne, Switzerland.
Haas
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D015107:4-Butyrolactone / Q000031:analogs & derivatives / Q000096:biosynthesis / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D006856:Hydrogen Cyanide / Q000378:metabolism*; D009097:Multienzyme Complexes / Q000096:biosynthesis / Q000235:genetics*; D010088:Oxidoreductases / Q000096:biosynthesis / Q000235:genetics*; D050891:Oxidoreductases Acting on CH-NH2 Group Donors; D011550:Pseudomonas aeruginosa / Q000201:enzymology / Q000235:genetics*; D012323:RNA Processing, Post-Transcriptional
D001426:Bacterial Proteins; C091100:GacA protein, Bacteria; D009097:Multienzyme Complexes; C092312:N-butyrylhomoserine lactone; C119941:RhiI protein, Rhizobium leguminosarum; D006856:Hydrogen Cyanide; D010088:Oxidoreductases; D050891:Oxidoreductases Acting on CH-NH2 Group Donors; C112800:glycine dehydrogenase (cyanide-forming); D015107:4-Butyrolactone