Secondary ocular involvement in systemic "memory" B-cell lymphocytic leukemia.
OBJECTIVE
Recently, B-cell chronic lymphocytic leukemia (B-CLL) has been subdivided into "naive" B-CLL and "memory" B-CLL on the basis of the presence of somatic mutations in the variable region of the immunoglobulin heavy chain gene (IgH). The aim of the current paper was to report the clinical, histopathologic, and molecular biologic findings of intraocular and ocular adnexal involvement in a patient with systemic B-CLL.


METHODS
Case report.


METHODS
Treatment consisted of systemic chemotherapy, conjunctival biopsies, and, ultimately, enucleation of the left eye.


METHODS
Histopathologic findings of a bone marrow biopsy, the conjunctival biopsies, and the enucleated eye were compared. Further, extensive immunohistochemistry, polymerase chain reaction (PCR) for the detection of heavy chain (IgH) gene rearrangement, gene scan analysis, and DNA sequencing were performed on all tissues.


RESULTS
The tumor manifestations in all specimens demonstrated similar morphologic and immunophenotypic characteristics, consistent with the diagnosis of B-CLL. Immunoglobulin-H PCR and gene scan analysis showed that the B-CLL infiltrates consisted of B cells derived from the same clone. DNA sequencing demonstrated the presence of eight somatic mutations in the variable region of IgH, consistent with "memory" B-CLL.


CONCLUSIONS
Secondary ocular manifestations of B-CLL occur relatively commonly during disease progression. In the current case of memory B-CLL, ocular manifestation of the disease occurred 16 years after initial diagnosis, agreeing with clinical studies suggesting that a less aggressive course is seen in "memory" B-CLL than its counterpart, "naive" B-CLL. Somatic mutation analysis in the variable region of IgH in B-CLL should be part of routine staging investigations to aid the prediction of the individual clinical course in B-CLL patients and to determine new therapeutic strategies.
Coupland
S E
SE
Department of Pathology, University Hospital Benjamin Franklin, Freie Universität, Berlin, Germany. secoupland@yahoo.de
Foss
H D
HD
Bechrakis
N E
NE
Hummel
M
M
Stein
H
H
D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000276:immunology; D001853:Bone Marrow / Q000473:pathology; D004252:DNA Mutational Analysis; D004273:DNA, Neoplasm / Q000032:analysis; D015353:Eye Enucleation; D005134:Eye Neoplasms / Q000235:genetics / Q000473:pathology* / Q000601:surgery; D005260:Female; D005784:Gene Amplification; D015326:Gene Rearrangement, B-Lymphocyte, Heavy Chain / Q000235:genetics; D005803:Genes, Immunoglobulin / Q000235:genetics; D006801:Humans; D007124:Immunoenzyme Techniques; D016130:Immunophenotyping; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics / Q000473:pathology* / Q000601:surgery; D008875:Middle Aged; D016133:Polymerase Chain Reaction
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D004273:DNA, Neoplasm