title: Macrophage migration inhibitory factor of the parasitic nematode Trichinella spiralis.
abstract: cDNAs were obtained for macrophage migration-inhibitory factor (MIF)/L-dopachrome methyl ester tautomerase homologues from the parasitic nematodes Trichinella spiralis (TsMIF) and Trichuris trichiura (TtMIF). The translated sequences, which were partly confirmed by sequencing of proteolytic fragments, show 42 and 44% identity respectively with human or mouse MIF, and are shorter by one C-terminal residue. Unlike vertebrate MIF and MIF homologues of filarial nematodes, neither TsMIF nor TtMIF contain cysteine residues. Soluble recombinant TsMIF, expressed in Escherichia coli showed secondary structure (by CD spectroscopy) and quaternary structure (by light-scattering and gel filtration) similar to that of the trimeric mammalian MIFs and D-dopachrome tautomerase. The catalytic specificity of recombinant TsMIF in the ketonization of phenylpyruvate (1.4x10(6) M(-1) x s(-1)) was comparable with that of human MIF, while that of p-hydroxyphenylpyruvate (9.1x10(4) M(-1) x s(-1)) was 71-fold lower. TsMIF showed high specificity in tautomerization of the methyl ester of L-dopachrome compared with non-esterified L-dopachrome (>87000-fold) and a high kcat (approximately 4x10(4) s(-1). The crystal structure, determined to 1.65 A (1 A=0.1 nm), was generally similar to that of human MIF, but differed in the boundaries of the putative active-site pocket, which can explain the low activity towards p-hydroxyphenylpyruvate. The central pore was blocked, but was continuous, with the three putative tautomerase sites. Recombinant TsMIF (5 ng/ml-5 pg/ml) inhibited migration of human peripheral-blood mononuclear cells in a manner similar to that shown by human MIF, but had no effect from 5 to 500 ng/ml on anti-CD3-stimulated murine T-cell proliferation. TsMIF was detected in supernatants of T. spiralis larvae cultured in vitro at 6 ng/ml (55 ng/mg total secreted protein). In conclusion TsMIF has structural, catalytic and cell-migration-inhibitory properties which indicate that it is partially orthologous to mammalian MIF.
authors: Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK.
authors: T H
authors: TH
authors: Tan
authors: S A
authors: SA
authors: Edgerton
authors: R
authors: R
authors: Kumari
authors: M S
authors: MS
authors: McAlister
authors: S M
authors: SM
authors: Roe
authors: S
authors: S
authors: Nagl
authors: L H
authors: LH
authors: Pearl
authors: M E
authors: ME
authors: Selkirk
authors: A E
authors: AE
authors: Bianco
authors: N F
authors: NF
authors: Totty
authors: C
authors: C
authors: Engwerda
authors: C A
authors: CA
authors: Gray
authors: D J
authors: DJ
authors: Meyer
authors: S M
authors: SM
authors: Rowe
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D018360:Crystallography, X-Ray; D018076:DNA, Complementary; D004926:Escherichia coli; D006801:Humans; D019746:Intramolecular Oxidoreductases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D007700:Kinetics; D007814:Larva; D008263:Macrophage Migration-Inhibitory Factors / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008958:Models, Molecular; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis; D020836:Protein Structure, Quaternary; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D017160:Trichinella spiralis / Q000235:genetics / Q000502:physiology*; D014258:Trichuris / Q000235:genetics / Q000502:physiology*; D014714:Vertebrates
chemical_list: D018076:DNA, Complementary; D008263:Macrophage Migration-Inhibitory Factors; D011994:Recombinant Proteins; D019746:Intramolecular Oxidoreductases; C042501:dopachrome isomerase