Serotonin transporter in rat platelets. Level of protein expression underlies inherited differences in uptake kinetics.
By breeding selection for the extreme values of platelet serotonin level (PSL), two sublines of Wistar-derived rats, with constitutionally high or low PSL and platelet serotonin uptake (PSU), have been developed. Searching for the basis of these differences, we performed quantitative western blot analysis of serotonin transporter (5HTt) in platelet membranes isolated from both rat sublines. A polyclonal anti-5HTt antibody labeled a single, 5HTt-related 94 kDa protein band in platelet membranes, with significantly stronger intensity in membranes from rats that exhibited a high PSL. We conclude that the inherited differences in PSL and PSU in rats, following breeding selection, are determined by the level of 5HTt expression in platelet membranes.
Hranilović
D
D
Laboratory of Neurochemistry and Molecular Neurobiology, Ruder Bosković Institute, Zagreb, Croatia.
Herak-Kramberger
C M
CM
Cicin-Sain
L
L
Sabolić
I
I
Jernej
B
B
D000818:Animals; D001692:Biological Transport; D001792:Blood Platelets / Q000378:metabolism*; D015153:Blotting, Western; D002352:Carrier Proteins / Q000097:blood* / Q000235:genetics* / Q000302:isolation & purification; D002462:Cell Membrane / Q000378:metabolism; D007700:Kinetics; D008297:Male; D008562:Membrane Glycoproteins / Q000097:blood* / Q000235:genetics* / Q000302:isolation & purification; D026901:Membrane Transport Proteins; D009419:Nerve Tissue Proteins; D051381:Rats; D017208:Rats, Wistar; D012701:Serotonin / Q000097:blood*; D050486:Serotonin Plasma Membrane Transport Proteins
D002352:Carrier Proteins; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D009419:Nerve Tissue Proteins; D050486:Serotonin Plasma Membrane Transport Proteins; C493232:Slc6a4 protein, rat; D012701:Serotonin