Molecular epidemiology of HIV type 1 subtypes in equatorial guinea.
Equatorial Guinea is endemic for HIV-1. This country borders to the north with Cameroon, where different subtypes belonging to group M, as well as group O strains, are circulating simultaneously. To assess the molecular epidemiology of HIV-1 in Equatorial Guinea we analyzed 76 plasma samples collected throughout 1999 from seropositive individuals. Phylogenetic analysis of the gp41 region revealed that 53 were of subtype A, with 64% of these sequences clustering with CRF02_AG reference strains; 11 were of subtype C; 4 were of subtype D; 2 (closely related to subtype F2) were of subtype F; 3 were of subtype G, two of them forming a separate cluster with the recombinant circulating forms CRF06_cpx; 1 was of subtype H; and 2 were unclassifiable. Although subtype A is predominant, the presence of 14% of subtype C is also noteworthy. This work represents the first HIV-1 subtype distribution study in Equatorial Guinea.
Ortiz
M
M
Servicio de Diagnóstico y Referencia de Retrovirus, Centro Nacional de Microbiologia, Instituto de Salud Carlos III, 28220 Majadahonda-Madrid, Spain. mortiz@isciii.es
Sanchez
I
I
Gonzalez
M P
MP
León
M I
MI
Abeso
N
N
Asumu
E
E
García-Sáiz
A
A
D001483:Base Sequence; D004279:DNA, Viral; D018984:Epitopes, T-Lymphocyte / Q000235:genetics; D016703:Equatorial Guinea / Q000453:epidemiology; D015700:HIV Envelope Protein gp41 / Q000145:classification / Q000235:genetics*; D006679:HIV Seropositivity / Q000097:blood / Q000453:epidemiology / Q000821:virology*; D015497:HIV-1 / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology
D004279:DNA, Viral; D018984:Epitopes, T-Lymphocyte; D015700:HIV Envelope Protein gp41