title: Msps/XMAP215 interacts with the centrosomal protein D-TACC to regulate microtubule behaviour.
abstract: The XMAP215/ch-TOG/Msps family of microtubule-associated proteins (MAPs) promote microtubule growth in vitro and are concentrated at centrosomes in vivo. We show here that Msps (mini-spindles protein) interacts with the centrosomal protein D-TACC, and that this interaction strongly influences microtubule behaviour in Drosophila embryos. If D-TACC levels are reduced, Msps does not concentrate at the centrosomes efficiently and the centrosomal microtubules appear to be destabilized. If D-TACC levels are increased, both D-TACC and Msps accumulate around the centrosomes/spindle poles, and the centrosomal microtubules appear to be stabilized. We show that the interaction between D-TACC and Msps is evolutionarily conserved. We propose that D-TACC and Msps normally cooperate to stabilize centrosomal microtubules by binding to their minus ends and binding to their plus ends as they grow out from the centrosome.
authors: Department of Genetics, Wellcome/CRC Institute, Tennis Court Road, Cambridge CB2 1QR, UK.
authors: M J
authors: MJ
authors: Lee
authors: F
authors: F
authors: Gergely
authors: K
authors: K
authors: Jeffers
authors: S Y
authors: SY
authors: Peak-Chew
authors: J W
authors: JW
authors: Raff
mesh_terms: D000818:Animals; D015153:Blotting, Western; D018385:Centrosome / Q000378:metabolism* / Q000648:ultrastructure; D003001:Cloning, Molecular; D004330:Drosophila / Q000196:embryology; D029721:Drosophila Proteins; D004347:Drug Interactions; D019143:Evolution, Molecular; D006801:Humans; D019476:Insect Proteins / Q000378:metabolism / Q000494:pharmacology; D008856:Microscopy, Fluorescence; D008869:Microtubule-Associated Proteins / Q000378:metabolism / Q000494:pharmacology*; D008870:Microtubules / Q000187:drug effects* / Q000378:metabolism; D011485:Protein Binding; D014162:Transfection; D029867:Xenopus Proteins
chemical_list: C486738:CKAP5 protein, Xenopus; D029721:Drosophila Proteins; D019476:Insect Proteins; D008869:Microtubule-Associated Proteins; C404402:TACC protein, Drosophila; D029867:Xenopus Proteins; C121511:msps protein, Drosophila