Sensitivity of the polymerase chain reaction for the diagnosis of cutaneous leishmaniasis due to Leishmania (Viannia) guyanensis.
The sensitivity of the polymerase chain reaction (PCR) in 35 consecutive outpatients with cutaneous leishmaniasis caused by Leishmania (Viannia) guyanensis was evaluated using, as gold standard, the in vitro isolation of the parasite through culture of aspirates of the cutaneous ulcers. All isolates were identified using electrophoretic enzyme analysis. Patients were mainly young males with recent onset disease without prior specific treatment. PCR was performed using DNA extracted from fresh frozen biopsies of cutaneous ulcers. The reaction used a pair of oligonucleotides that amplify the conserved region of the minicircle molecule. PCR showed 100% sensitivity (95% CI from 90.0 to 100.0). These results were similar to the visualization of amastigotes in imprint preparations of cutaneous biopsy tissue and the inoculation of biopsy material in golden hamsters. Despite the high sensitivity of the PCR, in this particular clinical setting of cutaneous leishmaniasis caused by L. (V.) guyanensis in the Brazilian Amazon, it appears that the method of choice for diagnosis should be the direct visualization of amastigotes using imprint preparations and the PCR reserved for those patients with negative imprint results.
Romero
G A
GA
Núcleo de Medicina Tropical, Universidade de Brasília, Campus Universitário, Asa Norte, P.O. Box 04517, DF. 70919-970, Brasília, Brazil. gromero@unb.br
Guerra
M V
MV
Paes
M G
MG
Cupolillo
E
E
Bentin Toaldo
C
C
Macêdo
V O
VO
Fernandes
O
O
D000293:Adolescent; D000328:Adult; D000818:Animals; D001938:Brazil / Q000453:epidemiology; D002648:Child; D016054:DNA, Protozoan / Q000032:analysis; D005260:Female; D006801:Humans; D018334:Leishmania guyanensis / Q000302:isolation & purification*; D016773:Leishmaniasis, Cutaneous / Q000175:diagnosis* / Q000453:epidemiology / Q000469:parasitology; D008297:Male; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D012883:Skin Ulcer / Q000469:parasitology
D016054:DNA, Protozoan