T cell receptor Vbeta expression in patients with allergic asthma before and after repeated low-dose allergen inhalation.
The objective of this study was to identify disease-associated T cell subsets by characterizing the lung and blood T cell receptor (TCR) repertoires in allergic asthmatics before and after repeated low-dose allergen challenge. Peripheral blood lymphocyte (PBL) and bronchoalveolar lavage (BAL) samples were obtained from eight patients with allergic asthma before and after a period of repeated low-dose allergen inhalations. RT-PCR followed by Southern blot allowed the quantification of relative Vbeta gene segment usage. Thirteen healthy individuals served as controls at PBL level. PBL as well as BAL T cells of asthmatics displayed a higher usage of Vbeta3, Vbeta5.2, and Vbeta6.1-3 and a lower usage of Vbeta16, Vbeta18, and Vbeta19 compared to PBL of healthy controls. Interestingly, TCR Vbeta7 and Vbeta9 usage was significantly higher in BAL than in PBL in asthmatics before as well as after challenge. TCR repertoire alterations after allergen challenge differed between individuals, with relatively mild changes.
Wahlström
J
J
Microbiology and Tumour Biology Centre, Karolinska Institutet, Stockholm, Sweden.
Gigliotti
D
D
Roquet
A
A
Wigzell
H
H
Eklund
A
A
Grunewald
J
J
D000280:Administration, Inhalation; D000328:Adult; D000485:Allergens / Q000008:administration & dosage; D001249:Asthma / Q000276:immunology* / Q000473:pathology; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D018076:DNA, Complementary / Q000235:genetics; D005260:Female; D015333:Gene Rearrangement, beta-Chain T-Cell Antigen Receptor; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000276:immunology* / Q000473:pathology; D016130:Immunophenotyping; D008168:Lung / Q000473:pathology; D008297:Male; D011058:Pollen / Q000276:immunology; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D000485:Allergens; D018076:DNA, Complementary; D016693:Receptors, Antigen, T-Cell, alpha-beta