Granulocyte integrins before and after activation in acute ischaemic stroke.
We examined in 19 subjects with acute ischaemic stroke (AIS) the PMN integrin pattern (CD11a, CD11b, CD11c, CD18), using indirect immunofluorescence and adopting a flow cytometer, at baseline and during activation, prolonged for 5 and 15 min, with 4-phorbol 12-myristate 13-acetate (PMA). At baseline, an increase in the expression of CD11c and CD18 and a decrease in the CD11b were evident in AIS subjects compared to normals. After activation, we found in normals a constant and significant increase of all PMN adhesive molecules, while in AIS subjects, we found an increase in CD11b and CD18, a decrease in CD11a and no variation in CD11c. While the basal upregulation of CD11c and CD18 may depend on the PMN spontaneous activation or on the increase of cytokines, the decrease of CD11b may be due to its self-consumption. After activation, the decrease in CD11a noted in AIS may be related to its cleavage or to an altered integrin phosphorylation/dephosphorylation balance.
Caimi
G
G
Istituto di Clinica Medica e Malattie Cardiovascolari, Università degli Studi di Palermo, Palermo, Italy. caimigre@unipa.it
Canino
B
B
Ferrara
F
F
Montana
M
M
Musso
M
M
Porretto
F
F
Carollo
C
C
Catania
A
A
Lo Presti
R
R
D000208:Acute Disease; D000368:Aged; D000369:Aged, 80 and over; D002545:Brain Ischemia / Q000276:immunology* / Q000378:metabolism*; D018821:CD18 Antigens / Q000378:metabolism; D002273:Carcinogens / Q000494:pharmacology; D002634:Chemotaxis, Leukocyte / Q000187:drug effects / Q000502:physiology; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006098:Granulocytes / Q000378:metabolism*; D006801:Humans; D016167:Integrin alphaXbeta2 / Q000378:metabolism; D016023:Integrins / Q000378:metabolism*; D016169:Lymphocyte Function-Associated Antigen-1 / Q000378:metabolism; D016177:Macrophage-1 Antigen / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D020521:Stroke / Q000276:immunology* / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D018821:CD18 Antigens; D002273:Carcinogens; D016167:Integrin alphaXbeta2; D016023:Integrins; D016169:Lymphocyte Function-Associated Antigen-1; D016177:Macrophage-1 Antigen; D013755:Tetradecanoylphorbol Acetate