Automated serological compatibility testing using a solid-phase test and standard laboratory equipment.
OBJECTIVE
To prove the feasibility of a semi-automated cross-match procedure using a commercially available solid-phase microplate test and standard laboratory equipment.


METHODS
The new procedure was evaluated against the conventional spin tube technique and the gel centrifugation system.


RESULTS
The sensitivity of the method and the rate of non-specific reactions were equal to those for the other test systems. The samples taken from the red cell concentrates for cross-matching remained stable for the shelf-life of the product.


CONCLUSIONS
The semi-automated cross-match was successfully introduced in our routine laboratory as a means to process large numbers of tests.
Ostendorf
N
N
Department of Transfusion Medicine and Transplantation Immunology, Westfaelische Wilhelms-Universitaet, Münster, Germany. transfusionsmedizin@uni-muenster.de
Niefhoff
D
D
Cassens
U
U
Sibrowski
W
W
D001788:Blood Grouping and Crossmatching / Q000295:instrumentation / Q000379:methods; D006801:Humans; D012680:Sensitivity and Specificity