The UDPase activity of the Kluyveromyces lactis Golgi GDPase has a role in uridine nucleotide sugar transport into Golgi vesicles.
In Saccharomyces cerevisiae a Golgi lumenal GDPase (ScGda1p) generates GMP, the antiporter required for entry of GDP-mannose, from the cytosol, into the Golgi lumen. Scgda1 deletion strains have severe defects in N- and O-mannosylation of proteins and glycosphingolipids. ScGda1p has also significant UDPase activity even though S. cerevisiae does not utilize uridine nucleotide sugars in its Golgi lumen. Kluyveromyces lactis, a species closely related to S. cerevisiae, transports UDP-N-acetylglucosamine into its Golgi lumen, where it is the sugar donor for terminal N-acetylglucosamine of the mannan chains. We have identified and cloned a K. lactis orthologue of ScGda1p. KlGda1p is 65% identical to ScGda1p and shares four apyrase conserved regions with other nucleoside diphosphatases. KlGda1p has UDPase activity as ScGda1p. Transport of both GDP-mannose, and UDP-GlcNAc was decreased into Golgi vesicles from Klgda1 null mutants, demonstrating that KlGda1p generates both GMP and UMP required as antiporters for guanosine and uridine nucleotide sugar transport into the Golgi lumen. Membranes from Klgda1 null mutants showed inhibition of glycosyltransferases utilizing uridine- and guanosine-nucleotide sugars, presumably due to accumulation of nucleoside diphosphates because the inhibition could be relieved by addition of apyrase to the incubations. KlGDA1 and ScGDA1 restore the wild-type phenotype of the other yeast gda1 deletion mutant. Surprisingly, KlGDA1 has only a role in O-glycosylation in K. lactis but also complements N-glycosylation defects in S. cerevisiae. Deletion mutants of both genes have altered cell wall stability and composition, demonstrating a broader role for the above enzymes.
Lopez-Avalos
M D
MD
Department of Molecular and Cell Biology, Boston University Goldman School of Dental Medicine, 700 Albany Street Boston, MA 02118, USA.
Uccelletti
D
D
Abeijon
C
C
Hirschberg
C B
CB
D000595:Amino Acid Sequence; D001483:Base Sequence; D001693:Biological Transport, Active; D002473:Cell Wall / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics; D017353:Gene Deletion; D005800:Genes, Fungal; D005816:Genetic Complementation Test; D006031:Glycosylation; D006056:Golgi Apparatus / Q000201:enzymology / Q000378:metabolism; D006150:Guanine Nucleotides / Q000378:metabolism; D007716:Kluyveromyces / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D008364:Mannosyltransferases / Q000037:antagonists & inhibitors; D008969:Molecular Sequence Data; D017351:N-Acetylglucosaminyltransferases / Q000037:antagonists & inhibitors; D011755:Pyrophosphatases / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D017386:Sequence Homology, Amino Acid; D014500:Uracil Nucleotides / Q000378:metabolism; D014537:Uridine Diphosphate N-Acetylglucosamine / Q000378:metabolism
D017931:DNA Primers; D006150:Guanine Nucleotides; D029701:Saccharomyces cerevisiae Proteins; D014500:Uracil Nucleotides; D014537:Uridine Diphosphate N-Acetylglucosamine; C068701:KRE2 protein, S cerevisiae; D008364:Mannosyltransferases; D017351:N-Acetylglucosaminyltransferases; D011755:Pyrophosphatases; C023273:uridine diphosphatase; C010357:guanosine-diphosphatase