Determination of phytic acid by gas chromatography-mass spectroscopy: application to biological samples.
A GC-MS method is reported for the determination of phytic acid based on purification by anion-exchange chromatography, enzymatic hydrolysis of phytic acid to myo-inositol and derivation to trimethylsilyl derivative, with scyllo-inositol as an internal standard. Analytical features of the method are: limit of detection 9 microg l(-1) phytic acid, linear working range 18-500 microg l(-1) phytic acid, and coefficient of variation 1.9%. The method has been successfully applied to a variety of biological samples: various rat organs (kidney, liver, brain and bone), human plasma and urine and kidney stones. A comparative study of sample treatments, including deproteization, lipid extraction and the presence of a chelator, is also reported. Phytic acid amounts found in rat organs ranged from 1.07 g kg(-1) for bone to 32.0 g kg(-1) for brain. Phytic acid in human plasma was of the order of 0.14 mg l(-1). In kidney stones, phytic acid was found in calcium containing stones.
March
J G
JG
Department of Chemistry , University of Balearic Islands, Palma de Mallorca, Spain. joan.march@clust.uib.es
Simonet
B M
BM
Grases
F
F
D000818:Animals; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D006868:Hydrolysis; D010833:Phytic Acid / Q000032:analysis* / Q000097:blood / Q000652:urine; D051381:Rats; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D010833:Phytic Acid