Application of genetic semihomology algorithm to theoretical studies on various protein families.
Several protein families of different nature were studied for genetic relationship, correct alignment at non-homologous fragments, optimal sequence consensus construction, and confirmation of their actual relevance. A comparison of the genetic semihomology approach with statistical approaches indicates a high accuracy and cognition significance of the former. This is particularly pronounced in the study of related proteins that show a low degree of homology. The sequence multiple alignments were verified and corrected with respect to the questionable, non-homologous fragments. The verified alignments were the basis for consensus sequence formation. The frequency of six-codon amino acids occurrence versus position variability was studied and their possible role in amino acid mutational exchange at variable positions is discussed.
Leluk
J
J
Institute of Biochemistry and Molecular Biology, University of Wrocław, Poland. lulu@bf.univ.wroc.pl
Hanus-Lorenz
B
B
Sikorski
A F
AF
D000465:Algorithms; D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry; D000818:Animals; D003062:Codon; D019992:Databases as Topic; D004912:Erythrocytes / Q000737:chemistry; D006801:Humans; D008957:Models, Genetic; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D017386:Sequence Homology, Amino Acid; D012984:Software; D013049:Spectrin / Q000737:chemistry
D000596:Amino Acids; D003062:Codon; D013049:Spectrin