The fission yeast ortholog of the coregulator SKIP interacts with the small subunit of U2AF.
The mode of action of transcriptional coregulators may involve the recruitment of spliceosome components. Using the two-hybrid screen, we examined the interaction partners of spSNW1, the S. pombe ortholog of the human coregulator SNW1/SKIP/NCoA-62, and found it to interact with the small subunit of the splicing factor U2AF (spU2AF23). The interaction involves the C-terminal parts of spU2AF23 and spSNW1. Tagged variants of both proteins were expressed in S. pombe and the interaction was proved by coprecipitation in nuclear extracts. This interaction would explain the finding of SKIP in nuclear speckles (Mintz, P. J., et al., EMBO J. 18, 4308-4320, 1999) and in reconstituted spliceosomes (Neubauer, G., et al., Nat. Genet. 20, 46-50, 1998). We deleted the spSNW1 gene in the diploid strain and demonstrated that spSNW1 is an essential gene in S. pombe.
Ambrozková
M
M
Department of Physiology and Developmental Biology, Charles University, Vinicná 7, Prague 2, 128 00, Czech Republic.
Půta
F
F
Fuková
I
I
Skruzný
M
M
Brábek
J
J
Folk
P
P
D002453:Cell Cycle; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism*; D012261:Ribonucleoproteins / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D012568:Schizosaccharomyces / Q000378:metabolism*; D000072668:Splicing Factor U2AF; D015533:Transcriptional Activation; D020798:Two-Hybrid System Techniques
D009687:Nuclear Proteins; D012261:Ribonucleoproteins; D000072668:Splicing Factor U2AF; C000607494:U2AF2 protein, human