The anti-idiotypic antibody to chlamydial glycolipid exoantigen (GLXA) protects mice against genital infection with a human biovar of Chlamydia trachomatis.
Despite more than three decades of anti-chlamydial vaccine research and improved vaccine strategies with new technologies, no vaccine candidate has protected against heterologous challenge, nor at more than one site of infection. The majority of experimental anti-chlamydial vaccines to date have targeted the chlamydial major outer membrane protein (MOMP). Many MOMP-directed vaccine candidates have been highly immunogenic, but have failed to protect against infectious challenge. We have extended our previous studies of a different anti-chlamydial vaccine, a monoclonal anti-idiotypic antibody (anti-Id; mAb2) which is a molecular mimic of the chlamydial glycolipid exoantigen (GLXA). The present studies demonstrate that the mAb2 vaccine is protective in a murine genital infection model utilizing a human urogenital strain. After either mucosal (oral or intranasal) or systemic (subcutaneous) immunization with the poly (lactide) encapsulated-mAb2 to GLXA, C3H/HeJ mice were significantly protected against topical vaginal challenge with Chlamydia trachomatis (K serovar; UW-31). Reduced vaginal shedding of organism and genital tract inflammation were associated with GLXA-specific and/or anti-EB neutralizing serum antibody. Our results demonstrate that the anti-Id (mAb2) vaccine is protective against an additional human biovar of C. trachomatis in C3H/HeJ mice, which are allogeneic to the source of mAb2 (BALB/c).
Whittum-Hudson
J A
JA
Department of Internal Medicine, Wayne State University School of Medicine, 119 Lande-Immunology, 550 East Canfield Avenue, Detroit, MI 48201, USA. jhudson@med.wayne.edu
Rudy
D
D
Gèrard
H
H
Vora
G
G
Davis
E
E
Haller
P K
PK
Prattis
S M
SM
Hudson
A P
AP
Saltzman
W M
WM
Stuart
E S
ES
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000008:administration & dosage*; D000907:Antibodies, Bacterial / Q000097:blood; D000911:Antibodies, Monoclonal / Q000008:administration & dosage; D001428:Bacterial Vaccines / Q000008:administration & dosage; D002690:Chlamydia Infections / Q000276:immunology* / Q000473:pathology / Q000517:prevention & control*; D002692:Chlamydia trachomatis / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D005260:Female; D019085:Fluorescent Antibody Technique, Direct; D005798:Genes, Bacterial; D005831:Genital Diseases, Female / Q000276:immunology* / Q000473:pathology / Q000517:prevention & control*; D006017:Glycolipids / Q000276:immunology*; D006801:Humans; D051379:Mice; D008809:Mice, Inbred C3H; D009500:Neutralization Tests; D016133:Polymerase Chain Reaction; D011135:Polysaccharides, Bacterial / Q000276:immunology*
D000888:Antibodies, Anti-Idiotypic; D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D001428:Bacterial Vaccines; D006017:Glycolipids; D011135:Polysaccharides, Bacterial; C101572:chlamydial exoglycolipid antigen