title: Individuality of Ag-selected and preimmune TCR repertoires.
abstract: We have combined flow cytometry and single-cell PCR to characterize the TCRBV repertoires selected by individual mice in a model CD8 response against a defined peptide/MHC complex (CW3 170-1 79/Kd). Ourresults established thatdifferent mice select individually distinct yet structurally similar CW3-specific repertoires. Repertoire selection appears to be flexible depending on the immunizing cell dose. Using a single-donor, matched-pair-recipient adoptive transfer strategy, we demonstrated that the CW3-specific TCR repertoires of normal mice are already distinct at the preimmune level. We combine our data with computer simulations to test models for the composition of an Ag-specific preimmune repertoire and its selection during an immune response.
authors: INSERM Unit 503, Lyon, France. maryanski@cervi-lyon.inserm.fr
authors: J L
authors: JL
authors: Maryanski
authors: V
authors: V
authors: Attuil
authors: A
authors: A
authors: Hamrouni
authors: M
authors: M
authors: Mutin
authors: M
authors: M
authors: Rossi
authors: A
authors: A
authors: Aublin
authors: P
authors: P
authors: Bucher
mesh_terms: D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D003198:Computer Simulation; D005434:Flow Cytometry; D015723:Gene Library; D015236:HLA-C Antigens / Q000276:immunology*; D006801:Humans; D051379:Mice; D016133:Polymerase Chain Reaction; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000276:immunology*
chemical_list: D015236:HLA-C Antigens; C038653:HLA-C*03 antigen; D016693:Receptors, Antigen, T-Cell, alpha-beta