Association of IL-8 and MCP-1 with the development of reexpansion pulmonary edema in rabbits.
BACKGROUND
The aim of this study is to determine the relationships between the cytokines and the inflammatory response in reexpansion pulmonary edema (RPE).


METHODS
We examined the cell population, epithelial permeability measured by Evans blue dye (EB), betaglucuronidase and cytokine concentrations in bronchoalveolar lavage fluid (BALF) and/or blood using a rabbit RPE model.


RESULTS
We confirmed that RPE is characterized by recruitment of polymorphonuclear leukocytes (PMNs), the release of PMN granular contents into the air spaces, and increased vascular permeability. These findings were highly correlated with increased interleukin-8 (IL-8) and monocyte chemoattractant protein 1 (MCP-1) concentrations in the BALF. Growth related oncogene (GRO) was detected in the BALF from only 2 of the 7 reexpanded lungs while TNFalpha was not detected in any rabbits. A similar but less severe inflammatory response to the reexpanded lung was found in the contralateral lung.


CONCLUSIONS
IL-8 and MCP-1 may play important roles in the development of RPE; the inflammatory response is independent of TNFalpha and unilateral reexpansion of the lung induces an inflammatory response not only in the reexpanded lung but also in the contralateral lung.
Sakao
Y
Y
Department of Biochemistry, The University of Texas Health Center at Tyler, 75708-3154, USA.
Kajikawa
O
O
Martin
T R
TR
Nakahara
Y
Y
Hadden
W A
WA
Harmon
C L
CL
Miller
E J
EJ
D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology; D002199:Capillary Permeability / Q000276:immunology; D018932:Chemokine CCL2 / Q000097:blood*; D005260:Female; D016209:Interleukin-8 / Q000097:blood*; D008168:Lung / Q000098:blood supply; D009504:Neutrophils / Q000276:immunology; D001261:Pulmonary Atelectasis / Q000276:immunology*; D011654:Pulmonary Edema / Q000276:immunology*; D011817:Rabbits; D018746:Systemic Inflammatory Response Syndrome / Q000276:immunology*
D018932:Chemokine CCL2; D016209:Interleukin-8