Individuality of Ag-selected and preimmune TCR repertoires.
We have combined flow cytometry and single-cell PCR to characterize the TCRBV repertoires selected by individual mice in a model CD8 response against a defined peptide/MHC complex (CW3 170-1 79/Kd). Ourresults established thatdifferent mice select individually distinct yet structurally similar CW3-specific repertoires. Repertoire selection appears to be flexible depending on the immunizing cell dose. Using a single-donor, matched-pair-recipient adoptive transfer strategy, we demonstrated that the CW3-specific TCR repertoires of normal mice are already distinct at the preimmune level. We combine our data with computer simulations to test models for the composition of an Ag-specific preimmune repertoire and its selection during an immune response.
Maryanski
J L
JL
INSERM Unit 503, Lyon, France. maryanski@cervi-lyon.inserm.fr
Attuil
V
V
Hamrouni
A
A
Mutin
M
M
Rossi
M
M
Aublin
A
A
Bucher
P
P
D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D003198:Computer Simulation; D005434:Flow Cytometry; D015723:Gene Library; D015236:HLA-C Antigens / Q000276:immunology*; D006801:Humans; D051379:Mice; D016133:Polymerase Chain Reaction; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000276:immunology*
D015236:HLA-C Antigens; C038653:HLA-C*03 antigen; D016693:Receptors, Antigen, T-Cell, alpha-beta