title: Transcripts of the malaria vector Anopheles gambiae that are differentially regulated in the midgut upon exposure to invasive stages of Plasmodium falciparum.
abstract: Understanding the interactions between the most deadly malaria parasite, Plasmodium falciparum, and its main vector, Anopheles gambiae, would be of great help in developing new malaria control strategies. The malaria parasite undergoes several developmental transitions in the mosquito midgut and suffers population losses to which mosquito factors presumably contribute. To identify such factors, we analysed An. gambiae midgut transcripts whose expression is regulated upon ingestion of invasive or non-invasive forms of P. falciparum using a differential display approach. Sixteen cDNA were studied in detail; 12 represent novel genes of An. gambiae including a gene encoding profilin. Four transcripts were specifically regulated by P. falciparum gametocytes (invasive forms), whereas the others were regulated by either non-invasive or both non-invasive and invasive forms of the parasite. This differential regulation of some genes may reflect the adaptation of P. falciparum to its natural vector. These genes may be involved in the development of P. falciparum in An. gambiae or in the defence reaction of the mosquito midgut towards the parasite.
authors: Ecologie des Systèmes Vectoriels, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris Cedex 15, France. IRD-OCEAC, PO Box 288, Yaoundé, Cameroon.
authors: S
authors: S
authors: Bonnet
authors: G
authors: G
authors: Prévot
authors: J C
authors: JC
authors: Jacques
authors: C
authors: C
authors: Boudin
authors: C
authors: C
authors: Bourgouin
mesh_terms: D000818:Animals; D000852:Anopheles / Q000235:genetics* / Q000469:parasitology* / Q000502:physiology; D001769:Blood / Q000469:parasitology; D004064:Digestive System / Q000469:parasitology*; D004068:Digestive System Physiological Phenomena; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods; D006801:Humans; D007303:Insect Vectors / Q000235:genetics* / Q000502:physiology; D008297:Male; D008969:Molecular Sequence Data; D010963:Plasmodium falciparum / Q000472:pathogenicity*; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic; D014357:Trypsin / Q000235:genetics
chemical_list: D014357:Trypsin