cDNA cloning, chromosomal localization and evolutionary analysis of mouse vacuolar ATPase subunit D, Atp6m.
The multi-subunit vacuolar ATPase pump uses ATP hydrolysis to move protons into membrane bound compartments. The pump is involved in a variety of cellular functions, including regulation of cytosolic pH, vesicular transport, endocytosis, secretion, and apoptosis. Here, we describe the cDNA cloning and chromosomal mapping of subunit D of murine V-ATPase. The mouse gene, designated Atp6m, maps to Chromosome 12, in a region of high homology with human chromosome 14q24. Evolutionary analysis of subunit D orthologs in a variety of other species reveals that this is a highly conserved protein that has been under remarkably strong negative selection during evolution, most likely reflecting its critical role in multiple cellular processes.
Kennell
J A
JA
Department of Cell and Developmental Biology, University of Michigan, Ann Arbor, MI 48109-0616, USA.
Richards
N W
NW
Schaner
P E
PE
Gumucio
D L
DL
D000251:Adenosine Triphosphatases / Q000737:chemistry / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002883:Chromosomes, Human, Pair 14 / Q000235:genetics; D003001:Cloning, Molecular; D017124:Conserved Sequence / Q000235:genetics; D003433:Crosses, Genetic; D018076:DNA, Complementary / Q000235:genetics; D019143:Evolution, Molecular; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D025261:Mitochondrial Proton-Translocating ATPases; D008969:Molecular Sequence Data; D010802:Phylogeny; D021122:Protein Subunits; D016415:Sequence Alignment
D018076:DNA, Complementary; C501005:MT-ATP6 protein, human; D021122:Protein Subunits; D000251:Adenosine Triphosphatases; D025261:Mitochondrial Proton-Translocating ATPases; C501006:mt-Atp6 protein, mouse