Ascertainment of D-amino acids in germ-free, gnotobiotic and normal laboratory rats.
Free D-amino acids were ascertained in the blood serum, urine and aqueous ethanolic extracts of feces of germ-free laboratory rats and a rat made gnotobiotic (tetra-associated) with species of Streptococcus, Lactobacillus and Clostridium. D-Amino acids were also determined in the brains of two germ-free rats. For comparison, D-amino acids were also measured in the blood serum of normal rats and the blood plasma, urine and feces of normal white mice. D-Enantiomers of most protein L-amino acids were detected in all physiological samples of animals. Quantities of free D-amino acids were determined as N(O)-pentafluoropropionyl-(2)-propyl esters by enantioselective gas chromatography and mass spectrometry. Stereoisomers of the bacterial marker 2,6-diaminopimelic acid, analyzed as N-trifluoroacetyl-(2)-propyl esters, were detected in feces of the gnotobiotic but not of the germ-free rat.
Brückner
H
H
Department of Food Sciences, Institute of Nutritional Science, Interdisciplinary Research Center, Justus-Liebig-University of Giessen, 35392 Giessen, Germany. hans.brueckner@ernaehrung.uni-giessen.de
Schieber
A
A
D000596:Amino Acids / Q000032:analysis* / Q000097:blood; D000818:Animals; D003013:Clostridium / Q000302:isolation & purification; D005243:Feces / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry; D005856:Germ-Free Life; D007778:Lactobacillus / Q000302:isolation & purification; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013291:Streptococcus / Q000302:isolation & purification
D000596:Amino Acids