title: Relative abundance and inhibitory distribution of protease inhibitors in potato juice from cv. Elkana.
abstract: Protease inhibitors from potato juice of cv. Elkana were purified and quantified. The protease inhibitors represent ca. 50% of the total soluble proteins in potato juice. The protease inhibitors were classified into seven different families: potato inhibitor I (PI-1), potato inhibitor II (PI-2), potato cysteine protease inhibitor (PCPI), potato aspartate protease inhibitor (PAPI), potato Kunitz-type protease inhibitor (PKPI), potato carboxypeptidase inhibitor (PCI), and "other serine protease inhibitors". The most abundant families were the PI-2 and PCPI families, representing 22 and 12% of all proteins in potato juice, respectively. Potato protease inhibitors show a broad spectrum of enzyme inhibition. All the families (except PCI) inhibited trypsin and/or chymotrypsin. PI-2 isoforms exhibit 82 and 50% of the total trypsin and chymotrypsin inhibiting activity, respectively. A strong variation within the latter activities was shown within one family and between protease inhibitor families.
authors: Centre for Protein Technology, TNO-WU, P.O. Box 8129, 6700 EV Wageningen, The Netherlands.
authors: L
authors: L
authors: Pouvreau
authors: H
authors: H
authors: Gruppen
authors: S R
authors: SR
authors: Piersma
authors: L A
authors: LA
authors: van den Broek
authors: G A
authors: GA
authors: van Koningsveld
authors: A G
authors: AG
authors: Voragen
mesh_terms: D002918:Chymotrypsin / Q000037:antagonists & inhibitors; D013058:Mass Spectrometry; D011480:Protease Inhibitors / Q000145:classification / Q000302:isolation & purification*; D011198:Solanum tuberosum / Q000737:chemistry*; D014361:Trypsin Inhibitors
chemical_list: D011480:Protease Inhibitors; D014361:Trypsin Inhibitors; D002918:Chymotrypsin