Efficient transformation of filamentous fungus Pleurotus ostreatus using single-strand carrier DNA.
The effects of carrier DNAs on the transformation of the basidiomycete Pleurotus ostreatus were analyzed. When lambda phage DNA was added to a transformation mixture containing protoplasts and CbxR vector plasmid, an increased number of drug-resistant transformants was observed on a screening plate containing 2 microg carboxin/ml. The highest efficiency (about 200 transformants/microg vector plasmid) was obtained by the addition of heat-denatured lambda DNA, which gave yields approximately 50-fold higher than the control experiment without a carrier DNA. To our knowledge, this is the first report on enhancement in transformation efficiency of fungal protoplasts by single strand carrier DNA.
Irie
T
T
Wood Research Institute, Kyoto University, Japan.
Honda
Y
Y
Watanabe
T
T
Kuwahara
M
M
D010582:Bacteriophage lambda / Q000235:genetics; D001709:Biotechnology; D004277:DNA, Single-Stranded / Q000008:administration & dosage*; D004279:DNA, Viral / Q000008:administration & dosage / Q000235:genetics; D004337:Drug Carriers; D025141:Drug Resistance, Fungal / Q000235:genetics; D005800:Genes, Fungal; D005819:Genetic Markers; D005822:Genetic Vectors; D010957:Plasmids / Q000008:administration & dosage / Q000235:genetics; D020076:Pleurotus / Q000235:genetics*; D014170:Transformation, Genetic
D004277:DNA, Single-Stranded; D004279:DNA, Viral; D004337:Drug Carriers; D005819:Genetic Markers