Cloning and molecular characterization of three ubiquitin fusion degradation 1 (Ufd1) ortholog genes from Xenopus laevis, Gallus gallus and Drosophila melanogaster.
The yeast ubiquitin fusion degradation 1 (Ufd1) protein is involved in a degradation pathway for ubiquitin fused products. The human ortholog gene (UFD1-like, UFD1L) is deleted in patients affected by the DiGeorge/velocardiofacial syndromes. We report the cloning of UFD1L orthologs from Drosophila melanogaster (dufd1l), Xenopus laevis and Gallus gallus. The 1,125-bp Drosophila cDNA encodes a protein of 316 amino acids, showing 60% identity with the human and murine proteins. The identity to the G. gallus, X. laevis, C. elegans and S. cerevisiae proteins is 95%, 83%, 32%, and 36%, respectively. Northern expression data in Drosophila indicate that dufd1l is expressed through embryonic, larval and pupal development, as well as in the adult fly.
Ratti
A
A
Istituto di Clinica Neurologica - Università di Milano, Italy.
Amati
F
F
Bozzali
M
M
Conti
E
E
Sangiuolo
F
F
Berloco
M
M
Palumbo
G
G
Botta
A
A
Pizzuti
A
A
Novelli
G
G
Dallapiccola
B
B
D033942:Adaptor Proteins, Vesicular Transport; D000375:Aging / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D002645:Chickens / Q000235:genetics*; D003001:Cloning, Molecular; D017124:Conserved Sequence / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D004331:Drosophila melanogaster / Q000196:embryology / Q000235:genetics*; D020224:Expressed Sequence Tags; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D047908:Intracellular Signaling Peptides and Proteins; D007814:Larva / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D011506:Proteins / Q000737:chemistry / Q000235:genetics*; D011679:Pupa / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D029867:Xenopus Proteins; D014982:Xenopus laevis / Q000235:genetics*
D033942:Adaptor Proteins, Vesicular Transport; D018076:DNA, Complementary; D036341:Intercellular Signaling Peptides and Proteins; D047908:Intracellular Signaling Peptides and Proteins; D011506:Proteins; D012333:RNA, Messenger; C491498:UFD1 protein, Xenopus; C491497:UFD1 protein, human; C107396:Ufd1 protein, mouse; D029867:Xenopus Proteins