title: MBP fusion protein with a viral protease cleavage site: one-step cleavage/purification of insoluble proteins.
authors: Department of Biochemistry and Biophysics, Box 712, University of Rochester Medical Center, Rochester, NY 14642, USA. aalexand@mc.rochester.edu
authors: A
authors: A
authors: Alexandrov
authors: K
authors: K
authors: Dutta
authors: S M
authors: SM
authors: Pascal
mesh_terms: D000086042:3C Viral Proteases; D018528:ATP-Binding Cassette Transporters; D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D051017:Apoptosis Regulatory Proteins; D001483:Base Sequence; D002352:Carrier Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D003546:Cysteine Endopeptidases / Q000378:metabolism*; D018076:DNA, Complementary / Q000235:genetics; D004926:Escherichia coli; D029968:Escherichia coli Proteins; D015951:Factor Xa / Q000378:metabolism; D005813:Genes, Synthetic; D005822:Genetic Vectors / Q000235:genetics*; D006639:Histidine / Q000737:chemistry; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D058619:Maltose-Binding Proteins; D008969:Molecular Sequence Data; D009004:Monosaccharide Transport Proteins; D015202:Protein Engineering; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D012229:Rhinovirus / Q000201:enzymology; D012995:Solubility; D013379:Substrate Specificity; D014764:Viral Proteins / Q000378:metabolism*
chemical_list: D018528:ATP-Binding Cassette Transporters; D051017:Apoptosis Regulatory Proteins; D002352:Carrier Proteins; D018076:DNA, Complementary; D029968:Escherichia coli Proteins; D047908:Intracellular Signaling Peptides and Proteins; D058619:Maltose-Binding Proteins; D009004:Monosaccharide Transport Proteins; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins; C461599:maltose transport system, E coli; C101296:prostate apoptosis response-4 protein; D006639:Histidine; D015951:Factor Xa; D003546:Cysteine Endopeptidases; D000086042:3C Viral Proteases