Bioactive 5-reduced 16 alpha,17 alpha-cyclohexanoprogesterone derivatives weakly interact with proteins of the soluble uterine fraction.
We studied competitive activities of 16 alpha,17 alpha-cyclohexano-5 alpha- and 5 beta-dihydroprogesterone in replacing(3)H-progesterone and(3)H-16 alpha,17 alpha-cyclohexano-6 alpha-methylprogesterone from protein complexes. Direct binding of(3)H-5-reduced derivatives with proteins of soluble fractions from rat and rabbit uteri was also assayed. C(d) values for 5-reduced derivatives were in the micro- or submicromolar range. The data suggest that biological effects of these analogues are not mediated via soluble uterine receptors.
Smirnov
A N
AN
Laboratory of Endocrinology, Department of Biology, M. V. Lomonosov Moscow State University.
Pokrovskaya
E V
EV
Levina
I S
IS
Kulikova
L E
LE
Kamernitskii
A V
AV
Shevchenko
V P
VP
D000818:Animals; D005260:Female; D009124:Muscle Proteins / Q000378:metabolism*; D011374:Progesterone / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism*; D011817:Rabbits; D011869:Radioligand Assay; D051381:Rats; D014316:Tritium; D014599:Uterus / Q000378:metabolism*
D009124:Muscle Proteins; D014316:Tritium; C085261:pentarane A; D011374:Progesterone