Micronuclei and other nuclear lesions as genotoxicity indicators in rainbow trout Oncorhynchus mykiss.
The induction of micronuclei and other nuclear abnormalities in renal erythrocytes of rainbow trout Oncorhynchus mykiss by six genotoxic compounds is evaluated. Colchicine, mitomycin, cyclophosphamide, acrylamide, methyl-methanesulfonate, and N-ethyl-N-nitrosourea were intraperitoneally injected in trout. Our results show that cyclophosphamide induces the formation of micronuclei and also the other nuclear abnormalities; N-ethyl-N-nitrosourea, acrylamide, and colchicine induce only micronuclei; mitomycin-C induces only other nuclear abnormalities but not micronuclei. Methyl-methanesulfonate does not induce nuclear abnormalities in rainbow trout at the dose assayed in this work. The possible genotoxic origin for the different nuclear abnormalities is discussed.
Ayllón
F
F
Departamento de Biologia Funcional, Area de Genetica, Facultad de Medicina, Universidad de Oviedo, c/Julian Claveria s/n, 33006 Oviedo, Spain. ayllon@sauron.quimica.uniovi.es
Garcia-Vazquez
E
E
D020106:Acrylamide / Q000008:administration & dosage / Q000633:toxicity; D000818:Animals; D002467:Cell Nucleus / Q000187:drug effects*; D003078:Colchicine / Q000008:administration & dosage / Q000633:toxicity; D003520:Cyclophosphamide / Q000008:administration & dosage / Q000633:toxicity; D004912:Erythrocytes / Q000187:drug effects; D007274:Injections, Intraperitoneal; D008741:Methyl Methanesulfonate / Q000008:administration & dosage / Q000633:toxicity; D008770:Methylnitrosourea / Q000008:administration & dosage / Q000633:toxicity; D048629:Micronuclei, Chromosome-Defective / Q000187:drug effects*; D015162:Micronucleus Tests; D016685:Mitomycin / Q000008:administration & dosage / Q000633:toxicity; D009153:Mutagens / Q000008:administration & dosage / Q000633:toxicity*; D017686:Oncorhynchus mykiss / Q000502:physiology
D009153:Mutagens; D020106:Acrylamide; D016685:Mitomycin; D008770:Methylnitrosourea; D003520:Cyclophosphamide; D008741:Methyl Methanesulfonate; D003078:Colchicine