Cytosolic phospholipase A(2)and 15-hydroxyprostaglandin dehydrogenase mRNA expression in murine uterine and gestational tissues during late pregnancy.
We evaluated the changes in mRNA expression of cytosolic phospholipase A(2)(cPLA(2)) and 15-hydroxyprostaglandin dehydrogenase (PGDH) in intrauterine and gestational tissues during mid-late murine pregnancy. Tissues (decidual caps, fetal membranes, and placentae, uterus, and cervix) were collected from pregnant mice at days 12, 14, 16, 18, and 19 (am and pm) of gestation. Total RNA was isolated and evaluated for cPLA(2)and PGDH expression by northern blot analysis normalized to GAPDH expression. Expression of mRNA for cPLA(2)increased in the placentae and decidual caps on day 18 and 19 pm, respectively. There was also increased expression for PGDH mRNA in the placenta and fetal membranes at the later stages of pregnancy. The tissue specific differences in expression of cPLA(2)and PGDH suggest that changes in enzymatic regulation of PG production and degradation may be crucial for the initiation of labour.
Sato
T A
TA
Division of Pharmacology and Clinical Pharmacology and Liggins Institute, University of Auckland, Auckland, New Zealand. t.sato@auckland.ac.nz
Gupta
D K
DK
Keelan
J A
JA
Marvin
K W
KW
Mitchell
M D
MD
D000818:Animals; D015152:Blotting, Northern; D003600:Cytosol / Q000378:metabolism*; D004622:Embryo, Mammalian / Q000378:metabolism*; D005260:Female; D006911:Hydroxyprostaglandin Dehydrogenases / Q000096:biosynthesis*; D051379:Mice; D008809:Mice, Inbred C3H; D010741:Phospholipases A / Q000096:biosynthesis*; D011247:Pregnancy; D012313:RNA / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D013997:Time Factors; D014599:Uterus / Q000378:metabolism*
D012333:RNA, Messenger; D012313:RNA; D006911:Hydroxyprostaglandin Dehydrogenases; C035859:15-hydroxyprostaglandin dehydrogenase; D010741:Phospholipases A