Glycoinositolphospholipids from Trypanosoma cruzi induce B cell hyper-responsiveness in vivo.
The surface of the protozoan Trypanosoma cruzi, the etiologic agent of Chagas' disease, is covered by a dense glycolipid layer, composed mainly by a structurally related family of glycoinositolphospholipids (GIPLs). In the present study we evaluated the in vivo effects of the GIPL on B cell function and immunoglobulin (Ig) secretion. We observed that GIPL injection led to a sustained increase in circulating IgM levels. B cells from GIPL injected mice showed higher response when activated in vitro with either LPS or dextran-conjugated anti-IgD antibodies or purified cytokines. GIPL purified from T. cruzi also showed an adjuvant effect, since this glycophospholipid boosted a polysaccharide-(TNP-Ficoll) induced IgG response. Taken together, our data indicate that T. cruzi-derived GIPL could be at least partially responsible for the remarkable B cell activation observed during T. cruzi acute infection in vivo.
Bilate
A M
AM
Instituto de Microbiologia, Prof. Paulo de Góes, CCS, Universidade Federal do Rio de Janeiro, Cidade Universitária, Rio de Janeiro, RJ, Brazil.
Previato
J O
JO
Mendonça-Previato
L
L
Peçanha
L M
LM
D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D005260:Female; D006017:Glycolipids / Q000276:immunology / Q000494:pharmacology*; D007075:Immunoglobulin M / Q000097:blood / Q000187:drug effects; D008070:Lipopolysaccharides; D008213:Lymphocyte Activation / Q000187:drug effects; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010743:Phospholipids / Q000276:immunology / Q000494:pharmacology*; D014349:Trypanosoma cruzi / Q000737:chemistry*
D006017:Glycolipids; D007075:Immunoglobulin M; D008070:Lipopolysaccharides; D010743:Phospholipids; C115336:glycoinositolphospholipid, Trypanosoma