Determining the procedure for routine residue monitoring of sulfamethazine in edible animal tissues.
A simplified method to determine/identify residual sulfamethazine (SMZ) in edible tissues from cattle, pigs, chickens and sheep by a high-performance liquid chromatography (HPLC) with a photo-diode array detector is presented. The sample preparation was performed by homogenizing with 30% (v/v) ethanol in water followed by an Ultrafree-MC/PL as a centrifugal ultra-filtration unit. For determination/identification of SMZ, a reversed-phase C(4) column and a mobile phase of 15% (v/v) ethanol in water with a photo-diode array detector was used. Average recoveries from spiked SMZ (0.1-1.0 mg kg(-1)) were >or=80% with coefficients of variation between 1.3 and 4.3%. The limits of quantitation were calculated to be 0.057-0.060 mg kg(-1). The total time and solvent required for the analysis of one sample were <40 min and <2 mL of ethanol, respectively.
Furusawa
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Faculty of Human Life Science, Osaka City University, Sumiyoshi-ku, Osaka, Japan. furusawa@life.osaka-cu.ac.jp
D000818:Animals; D000890:Anti-Infective Agents / Q000032:analysis*; D002417:Cattle; D002645:Chickens; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004350:Drug Residues / Q000032:analysis*; D008461:Meat Products / Q000032:analysis*; D012015:Reference Standards; D012680:Sensitivity and Specificity; D012756:Sheep; D013418:Sulfamethazine / Q000032:analysis*; D013552:Swine
D000890:Anti-Infective Agents; D013418:Sulfamethazine