Effect of bile acids on formation of azoxymethane-induced aberrant crypt foci in colostomized F344 rat colon.
The effect of bile acids on the formation of azoxymethane induced aberrant crypt foci (ACF) was investigated using the fecal stream-excluded colons of colostomized F344 rats. The excluded colon was irrigated with saline or bile acids (1 mg/0.5 ml per day, 5 days/week) for 4 weeks. The mean numbers of ACF per colon in rats given cholic acid, deoxycholic acid (DCA), chenodeoxycholic acid (CDCA), lithocholic acid, and ursodeoxycholic acid (UDCA) were 160.8, 118.2, 227.8, 150.7 and 87.3, respectively, while that of the control was 174.0. The number of ACF was significantly larger in CDCA, but smaller in UDCA and DCA-treated rats than the control (P<0.01). DCA did not induce apoptosis in the colon under the present conditions.
Lin
C X
CX
Second Department of Surgery, School of Medicine, University of Tokushima, 3-chome, Kuramoto-cho, 770-8503, Tokushima, Japan.
Umemoto
A
A
Seraj
M J
MJ
Mimura
S
S
Monden
Y
Y
D000818:Animals; D017209:Apoptosis; D001397:Azoxymethane / Q000494:pharmacology*; D001647:Bile Acids and Salts / Q000627:therapeutic use*; D002635:Chenodeoxycholic Acid / Q000627:therapeutic use; D002756:Cholagogues and Choleretics / Q000627:therapeutic use; D019826:Cholic Acid / Q000627:therapeutic use; D003106:Colon / Q000002:abnormalities* / Q000187:drug effects / Q000473:pathology*; D003840:Deoxycholic Acid / Q000627:therapeutic use; D003902:Detergents / Q000627:therapeutic use; D004032:Diet; D004305:Dose-Response Relationship, Drug; D005765:Gastrointestinal Agents / Q000627:therapeutic use; D008095:Lithocholic Acid / Q000627:therapeutic use; D008297:Male; D009092:Mucous Membrane / Q000187:drug effects; D051381:Rats; D011916:Rats, Inbred F344; D014580:Ursodeoxycholic Acid / Q000627:therapeutic use
D001647:Bile Acids and Salts; D002756:Cholagogues and Choleretics; D003902:Detergents; D005765:Gastrointestinal Agents; D003840:Deoxycholic Acid; D002635:Chenodeoxycholic Acid; D008095:Lithocholic Acid; D014580:Ursodeoxycholic Acid; D019826:Cholic Acid; D001397:Azoxymethane