title: Infrared-thermographic screening of the activity and enantioselectivity of enzymes.
abstract: The infrared radiation caused by the heat of reaction of an enantioselective enzyme-catalyzed transformation can be detected by modern photovoltaic infrared (IR)-thermographic cameras equipped with focal-plane array detectors. Specifically, in the lipase-catalyzed enantioselective acylation of racemic 1-phenylethanol, the (R)- and (S)-substrates are allowed to react separately in the wells of microtiter plates, the (R)-alcohol showing hot spots in the IR-thermographic images. Thus, highly enantioselective enzymes can be identified at kinetic resolution.
authors: Max-Planck-Institut für Kohlenforschung, Germany. reetz@mpi-muelheim.mpg.de
authors: M T
authors: MT
authors: Reetz
authors: M
authors: M
authors: Hermes
authors: M H
authors: MH
authors: Becker
mesh_terms: D001709:Biotechnology; D002384:Catalysis; D004798:Enzymes / Q000378:metabolism*; D066298:In Vitro Techniques; D007259:Infrared Rays; D007700:Kinetics; D013237:Stereoisomerism; D013817:Thermography / Q000379:methods*
chemical_list: D004798:Enzymes