High-level expression of a foreign gene from the most 3'-proximal locus of a recombinant Newcastle disease virus.
A previous report showed that insertion of a foreign gene encoding chloramphenicol acetyltransferase (CAT) between the HN and L genes of the full-length cDNA of a virulent Newcastle disease virus (NDV) yielded virus with growth retardation and attenuation. The NDV vector used in that study was pathogenic to chickens; it is therefore not suitable for use as a vaccine vector. In the present study, an avirulent NDV vector was generated and its potential to express CAT protein was evaluated. The CAT gene was under the control of NDV transcriptional start and stop signals and was inserted immediately before the open reading frame of the viral 3'-proximal nucleocapsid protein gene. A recombinant NDV expressing CAT activity at a high level was recovered. The replication and pathogenesis of the CAT-expressing recombinant NDV were not modified significantly. These results indicate the potential utility of an avirulent NDV as a vaccine vector.
Huang
Zhuhui
Z
Virginia-Maryland Regional College of Veterinary Medicine, University of Maryland, College Park, MD 20742, USA1.
Krishnamurthy
Sateesh
S
Virginia-Maryland Regional College of Veterinary Medicine, University of Maryland, College Park, MD 20742, USA1.
Panda
Aruna
A
Virginia-Maryland Regional College of Veterinary Medicine, University of Maryland, College Park, MD 20742, USA1.
Samal
Siba K
SK
Virginia-Maryland Regional College of Veterinary Medicine, University of Maryland, College Park, MD 20742, USA1.
D000818:Animals; D002645:Chickens; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics; D005822:Genetic Vectors; D009521:Newcastle Disease / Q000517:prevention & control; D009522:Newcastle disease virus / Q000235:genetics* / Q000472:pathogenicity; D019590:Nucleocapsid Proteins; D016366:Open Reading Frames; D011995:Recombination, Genetic; D014162:Transfection; D014774:Virulence; D014779:Virus Replication
D018076:DNA, Complementary; D019590:Nucleocapsid Proteins; D015500:Chloramphenicol O-Acetyltransferase