Time-resolved fluorescence emission measurements of photosystem I particles of various cyanobacteria: a unified compartmental model.
Photosystem I (PS-I) contains a small fraction of chlorophylls (Chls) that absorb at wavelengths longer than the primary electron donor P700. The total number of these long wavelength Chls and their spectral distribution are strongly species dependent. In this contribution we present room temperature time-resolved fluorescence data of five PS-I core complexes that contain different amounts of these long wavelength Chls, i.e., monomeric and trimeric photosystem I particles of the cyanobacteria Synechocystis sp. PCC 6803, Synechococcus elongatus, and Spirulina platensis, which were obtained using a synchroscan streak camera. Global analysis of the data reveals considerable differences between the equilibration components (3.4-15 ps) and trapping components (23-50 ps) of the various PS-I complexes. We show that a relatively simple compartmental model can be used to reproduce all of the observed kinetics and demonstrate that the large kinetic differences are purely the result of differences in the long wavelength Chl content. This procedure not only offers rate constants of energy transfer between and of trapping from the compartments, but also well-defined room temperature emission spectra of the individual Chl pools. A pool of red shifted Chls absorbing around 702 nm and emitting around 712 nm was found to be a common feature of all studied PS-I particles. These red shifted Chls were found to be located neither very close to P700 nor very remote from P700. In Synechococcus trimeric and Spirulina monomeric PS-I cores, a second pool of red Chls was present which absorbs around 708 nm, and emits around 721 nm. In Spirulina trimeric PS-I cores an even more red shifted second pool of red Chls was found, absorbing around 715 nm and emitting at 730 nm.
Gobets
B
B
Division of Physics and Astronomy of the faculty of Sciences and Institute of Molecular Biological Sciences, Vrije Universiteit, 1081 HV Amsterdam, The Netherlands. bas@nat.vu.nl
van Stokkum
I H
IH
Rögner
M
M
Kruip
J
J
Schlodder
E
E
Karapetyan
N V
NV
Dekker
J P
JP
van Grondelle
R
R
D002734:Chlorophyll / Q000737:chemistry* / Q000378:metabolism*; D000458:Cyanobacteria / Q000737:chemistry*; D004735:Energy Transfer; D007700:Kinetics; D008954:Models, Biological; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry* / Q000378:metabolism*; D013050:Spectrometry, Fluorescence
D045322:Photosynthetic Reaction Center Complex Proteins; D002734:Chlorophyll