Equine rhinitis A virus: structural proteins and immune response.
Equine rhinitis A virus (ERAV) is a picornavirus that has been reclassified as a member of the Aphthovirus genus because of its resemblance to foot-and-mouth disease virus at the level of nucleotide sequence and overall genomic structure. The N-terminal amino acid sequence of three of the four capsid proteins of ERAV was determined and showed that the proteolytic cleavage sites within the precursor P1 polypeptide occur exactly as those predicted for an aphthovirus-like 3C protease, which generates the capsid proteins VP1 and VP3. However, the autocatalytic cleavage site between VP4 and VP2, which is independent of 3C protease cleavage, was different from that predicted previously. ERAV.393/76 antisera from horses and rabbits showed different reactivity to the viral structural proteins in both serum neutralization assays and Western blots. High neutralizing antibody titres appeared to correlate with strong reactivity to VP1 in Western blots.
Hartley
Carol A
CA
Centre for Equine Virology, School of Veterinary Science, The University of Melbourne, Parkville, Victoria 3010, Australia1.
Ficorilli
Nino
N
Centre for Equine Virology, School of Veterinary Science, The University of Melbourne, Parkville, Victoria 3010, Australia1.
Dynon
Kemperly
K
Centre for Equine Virology, School of Veterinary Science, The University of Melbourne, Parkville, Victoria 3010, Australia1.
Drummer
Heidi E
HE
St Vincent's Institute of Medical Research, 9 Princes Street, Fitzroy, Victoria 3065, Australia2.
Huang
Jin-An
JA
Centre for Equine Virology, School of Veterinary Science, The University of Melbourne, Parkville, Victoria 3010, Australia1.
Studdert
Michael J
MJ
Centre for Equine Virology, School of Veterinary Science, The University of Melbourne, Parkville, Victoria 3010, Australia1.
D000086042:3C Viral Proteases; D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D000956:Antigens, Viral / Q000276:immunology; D015153:Blotting, Western; D002213:Capsid / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D003546:Cysteine Endopeptidases / Q000032:analysis; D006736:Horses; D007106:Immune Sera / Q000032:analysis; D008970:Molecular Weight; D009500:Neutralization Tests; D010849:Picornaviridae / Q000737:chemistry* / Q000276:immunology* / Q000528:radiation effects; D010850:Picornaviridae Infections / Q000276:immunology* / Q000821:virology; D011817:Rabbits; D014466:Ultraviolet Rays; D014764:Viral Proteins
D000914:Antibodies, Viral; D000956:Antigens, Viral; D007106:Immune Sera; D014764:Viral Proteins; D003546:Cysteine Endopeptidases; D000086042:3C Viral Proteases