Purification and characterization of anti-complementary polysaccharide from leaves of Thymus vulgaris L.
For the purification of the anti-complementary polysaccharide from Thymus vulgaris L., the hot-water extract of thyme leaves was successively fractionated by ethanol precipitation and ultra-filtration. The retentate with 300-kDa membrane cartridge showed a potent anti-complementary activity. It was further purified by open column chromatographies on DEAE-Toyopearl 650C, Butyl-Toyopearl 650M and Sephadex G-100, obtaining TV3-IIA-I, the purified anti-complementary polysaccharide. The anti-complementary polysaccharide exhibited the anti-complementary activity via both classical and alternative pathways. Based upon the methylation analysis and the reaction with beta-glucosyl Yariv reagent, the anti-complementary polysaccharide from thyme might contain an arabinogalactan moiety, at least in part.
Chun
H
H
Graduate School of Biotechnology, Korea University, Seoul.
Jun
W J
WJ
Shin
D H
DH
Hong
B S
BS
Cho
H Y
HY
Yang
H C
HC
D002852:Chromatography, Ion Exchange; D003169:Complement Inactivator Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D019686:Lamiaceae / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology
D003169:Complement Inactivator Proteins; D011134:Polysaccharides