Differentiation by in vitro treatment of lidocaine-epinephrine and prilocaine-felypressine in neutrophils.
Neutrophils are often the first cells of the immune system to encounter an invader, such as bacteria and fungi. Lidocaine-epinephrine induced transient potentiation of the production of superoxide anion, while prilocaine-felypressine induced persistent inhibition of the production in neutrophils. Moreover, lidocaine-epinephrine inhibited the production of hydrogen peroxide in spite that it potentiated the production of superoxide anion, while prilocaine-felypressine inhibited the production of hydrogen peroxide as well as superoxide anion. By contrast, lidocaine-epinephrine and prilocaine-felypressine are both effective in significantly inhibiting adhesion and phagocytosis. Using flow cytometric analysis, both local anesthetics were found to be effective in inhibiting the expression of Mac-1 (CD11b/CD18) in neutrophils. These results suggest that lidocaine-epinephrine and prilocaine-felypressine differentially modulate the production of superoxide anion, and could similarly inhibit adhesion, phagocytosis, and the production of hydrogen peroxide by neutrophils.
Azuma
Y
Y
Department of Pharmacology, Osaka Dental University, 8-1 Kuzuhahanazono-cho, Hirakata, 573-1121, Osaka, Japan. aazuma@cc.osaka-dent.ac.jp
Wang
P L
PL
Shinohara
M
M
Ohura
K
K
D000779:Anesthetics, Local / Q000494:pharmacology*; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D004837:Epinephrine / Q000494:pharmacology; D005259:Felypressin / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000378:metabolism; D008012:Lidocaine / Q000494:pharmacology; D016177:Macrophage-1 Antigen / Q000378:metabolism; D008297:Male; D018375:Neutrophil Activation / Q000187:drug effects; D009504:Neutrophils / Q000187:drug effects* / Q000276:immunology / Q000502:physiology; D010587:Phagocytosis / Q000187:drug effects; D011318:Prilocaine / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D013481:Superoxides / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D000779:Anesthetics, Local; D016177:Macrophage-1 Antigen; D011318:Prilocaine; D013481:Superoxides; D005259:Felypressin; D008012:Lidocaine; D006861:Hydrogen Peroxide; D013755:Tetradecanoylphorbol Acetate; D004837:Epinephrine