Intracellular pH-dependent efflux of the fluorescent probe pyranine in the yeast Yarrowia lipolytica.
8-Hydroxypyrene-1,3,6-trisulfonic acid (pyranine) can be used as a vital intracellular pH (pH(i)) indicator. In the yeast Yarrowia lipolytica, a partial efflux of the probe was detected by using the pH-independent wavelength of 415 nm. A simplified correction of the fluorescent signals was applied, enabling to show for this species a good near-neutral pH(i) maintenance capacity in a pH 3.9 medium. Octanoic acid, which is known to have toxic effects on yeast, decreased the pH(i) and increased the 260-nm-absorbing compounds leakage. However, this acid inhibited the fluorescent probe efflux linearly with its concentration suggesting a pH(i)-dependent efflux of pyranine from cells.
Aguedo
M
M
Laboratoire de Biotechnologie, Equipe IMSA, ENSBANA, 1, Esplanade Erasme, Université de Bourgogne, 21000, Dijon, France.
Waché
Y
Y
Belin
J M
JM
D001190:Arylsulfonates / Q000493:pharmacokinetics*; D001692:Biological Transport; D002210:Caprylates / Q000494:pharmacology; D002463:Cell Membrane Permeability / Q000187:drug effects; D003593:Cytoplasm / Q000378:metabolism; D005456:Fluorescent Dyes / Q000493:pharmacokinetics*; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D004718:Saccharomycetales / Q000378:metabolism*
D001190:Arylsulfonates; D002210:Caprylates; D005456:Fluorescent Dyes; C005047:pyranine; C031492:octanoic acid