[Influence of electroporation on the biological activities of primary rat hepatocytes].
OBJECTIVE
To investigate the influence of electroporation on the biological activities of primary rat hepatocyte and to optimize the electroporation conditions introducing foreign genes into hepatocytes.


METHODS
A single-pulse procedure was performed at low voltage (220-400 V) but high capacitance (500-950 microF). Its influence on hepatocyte activities was detected by Trypan blue exclusion (TBE) and MTT analysis. Besides, ALB, ALT and LDH in the supernatants of hepatocytes were tested by biochemical assay.


RESULTS
Little hepatocyte damage and high survival rate (>90%) was found from 36 hours till 9th day of culture. At 36th hour after electroporation, ALB, ALT and LDH in the supernatants of Group B (220V, 950 microF) and C (400 V, 950 microF) were higher than those of control group. Whereas TBE and MTT analysis failed to indicate the significant difference of cell viability between electroporation groups and control group.


CONCLUSIONS
This electroporation procedure is one of the optimal choices to introduce foreign genes into primary rat hepatocytes.
Yao
Y
Y
Institute for Viral Hepatitis, Chongqing University of Medical Sciences, Chongqing 400010, China.
Zhang
D
D
Luo
Y
Y
Zhang
D
D
Huang
A
A
Zhou
W
W
Ren
H
H
D000818:Animals; D002470:Cell Survival; D018274:Electroporation; D022761:Gene Transfer, Horizontal; D022781:Hepatocytes / Q000502:physiology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar