[Genetic analysis of 35 microsatellite loci in 5 lineages of xishuangbanna miniature pig inbred line].
The polymorphism of 35 microsatellites in 5 lineages of Xishuangbanna small-ear miniature pig inbred line (XMI) was analysed. Nmuber of alleles of each lineage was counted, and rates of homozygote for 35 microsatellite loci in 5 lineages were calculated. According to gene frequencies of 35 microsatellites polymorphism information content (PIC) and mean heterozygosity were calculated for each lineage, and genetic distances between these lineage were estimated. The dendrograms were obtained based on genetic distances. The results suggest that rates of homozygote in these lineage are all high, and that is the highest in lineage 151. The results also suggest that polymorphism information content and mean heterozygosity in all the lineages are low. Composition of alleles of each lineage was quite different and the genetic relationship between lineages accorded with the process of inbred line. So it is suggested that the inbreeding degree of 5 lineages of XMI are all high, and the richness of genetic diversity is lower than general commercial pig breeds. It also shows each lineage has been different groups with individual genes.
Niu
R
R
Laboratory Animal Centre, Third Military Medical University, Chongqing 400038, China.
Shang
H T
HT
Wei
H
H
Huang
Z B
ZB
Zeng
Y Z
YZ
D000483:Alleles; D000818:Animals; D002874:Chromosome Mapping; D018895:Microsatellite Repeats; D013552:Swine; D013556:Swine, Miniature / Q000235:genetics*