Use of cultured cells in assessing ethanol toxicity and ethanol-related metabolism.
This article represents the proceedings of a symposium at the 2000 ISBRA Meeting in Yokohama, Japan. The chairs were Terrence M. Donohue, Jr, and Dahn L. Clemens. The presentations were (1) Characterization of single and double recombinant hepatoma cells that express ethanol-metabolizing enzymes, by Terrence M. Donohue, Jr; (2) Inhibition of cell growth by ethanol metabolism, by Dahn L. Clemens; (3) Use of transfected HeLa cells to study the genesis of alcoholic fatty liver, by Andrea Galli and David Crabb; (4) CYP2E1-mediated oxidative stress induces COL1A2 mRNA in hepatic stellate cells and in a coculture system of HepG2 and stellate cells, by Natalia Nieto; (5) Transforming growth factor-alpha secreted from ethanol-exposed hepatocytes contributes to development of alcoholic hepatic fibrosis, by Junji Kato; and (6) Effect of ethanol on Fas-dependent caspase-3 activation and apoptosis in CD4+ T cells, by Shirish S. Barve.
Donohue
T M
TM
Liver Study Unit, Omaha Veterans Affairs Medical Center, University of Nebraska Medical Center 68105, USA. tdonohue@unc.edu
Clemens
D L
DL
Galli
A
A
Crabb
D
D
Nieto
N
N
Kato
J
J
Barve
S S
SS
D000079:Acetaldehyde / Q000378:metabolism; D000426:Alcohol Dehydrogenase / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D002492:Central Nervous System Depressants / Q000378:metabolism / Q000633:toxicity*; D019392:Cytochrome P-450 CYP2E1 / Q000187:drug effects / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism / Q000633:toxicity*; D005235:Fatty Liver, Alcoholic / Q000378:metabolism*; D006367:HeLa Cells / Q000187:drug effects; D022781:Hepatocytes / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D051381:Rats; D016211:Transforming Growth Factor alpha / Q000187:drug effects / Q000378:metabolism
D002492:Central Nervous System Depressants; D016211:Transforming Growth Factor alpha; D000431:Ethanol; D000426:Alcohol Dehydrogenase; D019392:Cytochrome P-450 CYP2E1; D000079:Acetaldehyde