Mapping and expression of microneme genes in Eimeria tenella.
Microneme organelles are located at the apical tip of invading stages of all apicomplexan parasites and they contain proteins that are critical for parasite adhesion to host cells. In this paper, we have utilised the process of oocyst sporulation in Eimeria tenella to investigate the timing of expression of components of the microneme organelle, at both mRNA and protein levels. Two time-course studies showed that there is a high level of synchrony in the sporulation process, especially during the time period when sporozoites are formed. Western blotting showed that the expression of five microneme proteins (EtMIC1-5) is differentially regulated and highly co-ordinated during sporulation with the proteins being detected only towards the end of the process, as the sporozoites matured within the sporocysts. In contrast, mRNA for all five of these microneme proteins was detected some 10-12 h earlier in sporulation than when the corresponding proteins were seen. Overall these data suggest that the expression of proteins destined for the microneme is regulated both at the transcriptional and translational level. The single copy genes encoding EtMIC1-5 are not clustered on the genome, but are found on four different chromosomes.
Ryan
R
R
Division of Molecular Biology, Institute for Animal Health Compton, Berkshire, UK.
Shirley
M
M
Tomley
F
F
D000818:Animals; D002874:Chromosome Mapping / Q000662:veterinary*; D018509:DNA, Helminth / Q000737:chemistry; D016786:Eimeria tenella / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field; D005786:Gene Expression Regulation; D015800:Protozoan Proteins / Q000096:biosynthesis / Q000235:genetics; D018510:RNA, Helminth / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary
D018509:DNA, Helminth; D015800:Protozoan Proteins; D018510:RNA, Helminth; D012333:RNA, Messenger