CD4(+) and CD8(+) T cell death during human immunodeficiency virus infection in vitro.
We have evaluated the death of CD4(+) and CD8(+) T cells during in vitro human immunodeficiency virus (HIV) infection of peripheral blood mononuclear cells (PBMC) and tonsilar tissue. Acute infections with several X4 and R5 HIV isolates induced a decrease in cell viability that was higher in infections with X4 viruses and correlated with an increased rate of CD4(+) T-cell death. In CD4(+) T cells, the primary X4 isolate AOM induced higher levels of death than the laboratory X4 isolates IIIB and NL4-3 or the R5 isolates BaL and MDM. An effect on CD8(+) T-cell viability was exclusively observed in infections by X4 viruses, including the NL4-3 strain, in both PBMC and tonsilar tissue. This effect was dependent on the env gene of the infecting isolate and required productive HIV replication in CD4(+) but not in CD8(+) T cells. Our results suggest that X4 and R5 HIV isolates depleted CD4(+) T cells to a different extent and that CD8(+) T-cell viability may also be affected by mechanisms other than those acting in CD4(+) T cells.
Blanco
J
J
Fundació irsiCaixa, Laboratori de Retrovirologia, Hospital Universitari Germans Trias i Pujol, Universitat Autònoma de Barcelona, Ctra. Canyet s/n, Badalona, Catalonia, 08916, Spain. jbalanco@ns.hugtip.scs.es
Barretina
J
J
Cabrera
C
C
Gutiérrez
A
A
Clotet
B
B
Esté
J A
JA
D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000821:virology*; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000821:virology*; D016923:Cell Death; D002470:Cell Survival; D002478:Cells, Cultured; D015699:HIV Envelope Protein gp120 / Q000235:genetics / Q000502:physiology; D015497:HIV-1 / Q000302:isolation & purification / Q000502:physiology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000821:virology; D008221:Lymphoid Tissue / Q000166:cytology; D010446:Peptide Fragments / Q000235:genetics / Q000502:physiology; D014779:Virus Replication
D015699:HIV Envelope Protein gp120; C059762:HIV envelope protein gp120 (305-321); D010446:Peptide Fragments