title: Complexes with truncated RNAs from the large domain of Archaeoglobus fulgidus signal recognition particle.
abstract: Protein SRP19 is an important component of the signal recognition particle (SRP) as it promotes assembly of protein SRP54 with SRP RNA and recognizes a tetranucleotide loop. Structural features and RNA binding activities of SRP19 of the hyperthermophilic archaeon Archaeoglobus fulgidus were investigated. An updated alignment of SRP19 sequences predicted three conserved regions and two alpha-helices. With Af-SRP RNA the Af-SRP54 protein assembled into an A. fulgidus SRP which remained intact for many hours. Stable complexes were formed between Af-SRP19 and truncated SRP RNAs, including a 36-residue fragment representing helix 6 of A. fulgidus SRP RNA.
authors: Department of Molecular Biology, The University of Texas Health Science Center at Tyler, 75708-3154, USA.
authors: S H
authors: SH
authors: Bhuiyan
authors: O N
authors: ON
authors: Pakhomova
authors: A P
authors: AP
authors: Hinck
authors: C
authors: C
authors: Zwieb
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D019611:Archaeoglobus fulgidus / Q000235:genetics*; D001419:Bacteria / Q000235:genetics; D001483:Base Sequence; D001665:Binding Sites; D017124:Conserved Sequence; D005658:Fungi / Q000737:chemistry; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D017433:Protein Structure, Secondary; D019642:RNA, Archaeal / Q000737:chemistry* / Q000302:isolation & purification; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D018271:Signal Recognition Particle / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D019642:RNA, Archaeal; C117074:SRP19 protein, human; D018271:Signal Recognition Particle