Genomic organization, transcription start sites, and chromosomal location of the Drosophila cortactin gene.
An actin filament binding protein cortactin was initially identified as a major phosphotyrosine-containing protein in v-Src-transformed chicken embryo fibroblast cells. The mouse, human, and Drosophila homologs were independently identified as a signaling molecule involved in a mitogenic response, as a product of a putative oncogene EMS1, and as a molecule interacting with a scaffolding protein ZO-1, respectively. In this report, we describe the cloning of the Drosophila cortactin gene, which consists of four exons and three introns, covering 3 kilobases in length. All exon-intron junctions are well matched with the GT/AG consensus sequence. S1 nuclease mapping revealed one major and several minor transcription start sites. The cytological location of the Drosophila cortactin gene is between chromosome segments 93B3 and 93B7.
Katsube
T
T
Neurogenetics Research Project, Mitsubishi Kasei Institute of Life Sciences, Machida-shi, Tokyo Japan. tkatsu@nirs.go.jp
Hashimoto
N
N
Sasaki
Y
Y
Yamashita
A
A
Takahisa
M
M
Ueda
R
R
Togashi
S
S
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D051356:Cortactin; D018076:DNA, Complementary / Q000378:metabolism; D004330:Drosophila / Q000235:genetics*; D029721:Drosophila Proteins; D005091:Exons; D017403:In Situ Hybridization; D007438:Introns; D008840:Microfilament Proteins / Q000235:genetics*; D008957:Models, Genetic; D008969:Molecular Sequence Data; D020033:Protein Isoforms; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015719:Single-Strand Specific DNA and RNA Endonucleases / Q000378:metabolism; D014158:Transcription, Genetic
D051356:Cortactin; D018076:DNA, Complementary; D029721:Drosophila Proteins; D008840:Microfilament Proteins; D020033:Protein Isoforms; C495866:cortactin protein, Drosophila; D015719:Single-Strand Specific DNA and RNA Endonucleases