title: Classical swine fever: pathogenesis of glomerular damage and immunocharacterization of immunocomplex deposits.
abstract: Twenty-six pigs were inoculated with a virulent isolate (Quillota strain) of classical swine fever (hog cholera) virus to determine the chronological development of lesions in the renal glomeruli and the pathogenesis of glomerular damage and immunocomplex deposition. The study included the use of histopathological, ultrastructural and immunohistochemical (detection of viral antigen gp55, myeloid-histiocyte antigen, IgM, IgG and C1q) techniques. The main changes in glomerular structure were observed from 7 days post-inoculation (dpi) onwards, at which time the glomeruli showed macrophage infiltrations in the mesangium, and viral infection in circulating cells, glomerular endothelial cells and podocytes. Moreover, significant subcellular changes were detected in podocytes, which appeared swollen, with fusion of foot processes. Immunocomplex deposits immunoreactive for IgM, IgG and C1q were detected in mesangial, subepithelial and subendothelial areas from 10 dpi, but viral antigen was not detected as a component of these deposits; fusion of foot processes had increased in severity, especially near immunocomplex deposits. All these changes had increased still further in the final phase of the experiment (14 dpi), with neutrophil infiltrations in the mesangium.
authors: Departamento de Anatomía Patológica, Facultad de Veterinaria, Universidad de Córdoba, Carretera de Madrid, 14014 Córdoba, Spain.
authors: E
authors: E
authors: Ruiz-Villamor
authors: M
authors: M
authors: Quezada
authors: M J
authors: MJ
authors: Bautista
authors: S
authors: S
authors: Romanini
authors: L
authors: L
authors: Carrasco
authors: F J
authors: FJ
authors: Salguero
authors: J C
authors: JC
authors: Gómez-Villamandos
mesh_terms: D000818:Animals; D000936:Antigen-Antibody Complex / Q000276:immunology*; D006691:Classical Swine Fever / Q000276:immunology* / Q000473:pathology / Q000821:virology; D006692:Classical Swine Fever Virus / Q000276:immunology / Q000302:isolation & purification / Q000502:physiology; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D005920:Glomerular Mesangium / Q000276:immunology / Q000648:ultrastructure / Q000821:virology; D005921:Glomerulonephritis / Q000276:immunology* / Q000473:pathology / Q000821:virology; D007124:Immunoenzyme Techniques / Q000662:veterinary; D007075:Immunoglobulin M / Q000032:analysis; D007678:Kidney Glomerulus / Q000276:immunology* / Q000473:pathology / Q000821:virology; D008297:Male; D008854:Microscopy, Electron / Q000662:veterinary; D013552:Swine
chemical_list: D000936:Antigen-Antibody Complex; D007075:Immunoglobulin M