Molecular cloning and characterization of chemokine-like factor 1 (CKLF1), a novel human cytokine with unique structure and potential chemotactic activity.
Cytokines are small proteins that have an essential role in the immune and inflammatory responses. The repertoire of cytokines is becoming diverse and expanding. Here we report the identification and characterization of a novel cytokine designated as chemokine-like factor 1 (CKLF1). The full-length cDNA of CKLF1 is 530 bp long and a single open reading frame encoding 99 amino acid residues. CKLF1 bears no significant similarity to any other known cytokine in its amino acid sequence. Expression of CKLF1 can be partly inhibited by interleukin 10 in PHA-stimulated U937 cells. Recombinant CKLF1 is a potent chemoattractant for neutrophils, monocytes and lymphocytes; moreover, it can stimulate the proliferation of murine skeletal muscle cells. These results suggest that CKLF1 might have important roles in inflammation and in the regeneration of skeletal muscle.
Han
W
W
Laboratory of Medical Immunology, School of Basic Medical Science, Peking University, 38 Xueyuan Road, Beijing, 100083, China.
Lou
Y
Y
Tang
J
J
Zhang
Y
Y
Chen
Y
Y
Li
Y
Y
Gu
W
W
Huang
J
J
Gui
L
L
Tang
Y
Y
Li
F
F
Song
Q
Q
Di
C
C
Wang
L
L
Shi
Q
Q
Sun
R
R
Xia
D
D
Rui
M
M
Tang
J
J
Ma
D
D
D000595:Amino Acid Sequence; D000818:Animals; D020029:Base Pairing; D001483:Base Sequence; D019556:COS Cells; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D018925:Chemokines / Q000737:chemistry / Q000235:genetics* / Q000494:pharmacology; D002633:Chemotaxis / Q000187:drug effects; D002634:Chemotaxis, Leukocyte / Q000187:drug effects / Q000502:physiology*; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004558:Electric Stimulation; D005091:Exons; D006801:Humans; D007438:Introns; D008214:Lymphocytes / Q000187:drug effects / Q000502:physiology; D062792:MARVEL Domain-Containing Proteins; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009000:Monocytes / Q000187:drug effects / Q000502:physiology; D018482:Muscle, Skeletal / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology; D016366:Open Reading Frames; D009928:Organ Specificity; D012333:RNA, Messenger / Q000235:genetics; D011994:Recombinant Proteins / Q000737:chemistry / Q000494:pharmacology; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic; D014162:Transfection; D020298:U937 Cells
C432676:CKLF protein, human; D018925:Chemokines; D018076:DNA, Complementary; D062792:MARVEL Domain-Containing Proteins; D012333:RNA, Messenger; D011994:Recombinant Proteins