Fluorescence characteristic study of the ternary complex of fluoroquinolone antibiotics and cobalt (II) with ATP.
The results from the measurement of the fluorescence spectra of fluoroquinolone antibiotics including ofloxacin (OF), norfloxacin (NOR) and ciprofloxacin (CIP) complexed with cobalt (II) and ATP give information concerning the antibiotics-nucleotide interactions. From the fluorescence spectral data, it appears that the fluoroquinolone antibiotic cannot directly complex with ATP but indirectly complex with cobalt (II), which is playing an intermediary role. The interaction of fluoroquinolone antibiotic with the nucleotide occurs mainly through the phosphate group. The conclusion offers a more complete mechanism, which is important for understanding the interaction of these drugs with DNA.
Wu
S
S
Department of Chemistry, Lanzhou University, People's Republic of China.
Zhang
W
W
Chen
X
X
Hu
Z
Z
Hooper
M
M
Hooper
B
B
Zhao
Z
Z
D000255:Adenosine Triphosphate / Q000737:chemistry*; D000890:Anti-Infective Agents / Q000737:chemistry*; D002939:Ciprofloxacin / Q000737:chemistry*; D003035:Cobalt / Q000737:chemistry*; D005453:Fluorescence; D015394:Molecular Structure; D009643:Norfloxacin / Q000737:chemistry*; D015242:Ofloxacin / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
D000890:Anti-Infective Agents; D003035:Cobalt; D002939:Ciprofloxacin; D000255:Adenosine Triphosphate; D015242:Ofloxacin; D009643:Norfloxacin