Large-scale preparation of the oligosaccharide phosphate fraction of Pichia holstii NRRL Y-2448 phosphomannan for use in the manufacture of PI-88.
Mild acid-catalysed hydrolysis of the extracellular phosphomannan of the yeast Pichia holstii NRRL Y-2448 produces a high-molecular-weight phosphomannan core, a low-molecular-weight oligosaccharide phosphate fraction, and a neutral oligosaccharide fraction. A method was developed for the large-scale preparation of the oligosaccharide phosphate fraction, consisting predominantly of the pentasaccharide phosphate, 6-O-PO3H2-alpha-D-Man-(1 --> 3)-alpha-D-Man-(1 --> 3)-alpha-D-Man-(1 C 3)-alpha-D-Man-(1 --> 2)-D-Man, for use in the manufacture of the promising new anti-cancer agent, PI-88. Further insights were also gained into the structure of the phosphomannan by HPLC analysis of the time course of the hydrolysis reaction.
Ferro
V
V
Department of Research and Development, Progen Industries Ltd, Richlands BC, Qld, Australia. vito.ferro@progen.com.au
Fewings
K
K
Palermo
M C
MC
Li
C
C
D000970:Antineoplastic Agents / Q000138:chemical synthesis*; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007218:Industrial Microbiology / Q000379:methods; D008351:Mannans / Q000737:chemistry / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D019906:Nuclear Magnetic Resonance, Biomolecular; D009844:Oligosaccharides / Q000138:chemical synthesis* / Q000737:chemistry / Q000302:isolation & purification*; D010843:Pichia / Q000737:chemistry*
C440773:6-phosphomannopyranosyl-1-3-mannopyranosyl-1-3-mannopyranosyl-1-3-mannopyranosyl-1-2-mannose; D000970:Antineoplastic Agents; D008351:Mannans; D009844:Oligosaccharides; C120158:phosphomannopentaose sulfate; C005437:phosphomannan