An upstream repressor element plays a role in Igf2 imprinting.
The imprinted Igf2 gene is associated with a small upstream region that is differentially methylated on the active paternal allele. We have identified a repressor element within this sequence and shown that repression is probably mediated through a trans- acting factor, GCF2. DNA methylation of this site abrogates both protein binding and repressor activity. Targeting experiments demonstrate that this element plays a role in the repression of the maternal Igf2 gene in vivo.
Eden
S
S
Department of Cellular Biochemistry, Hebrew University, Ein Kerem, Jerusalem 91120, Israel.
Constancia
M
M
Hashimshony
T
T
Dean
W
W
Goldstein
B
B
Johnson
A C
AC
Keshet
I
I
Reik
W
W
Cedar
H
H
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002478:Cells, Cultured; D002678:Chimera; D003433:Crosses, Genetic; D019175:DNA Methylation; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology; D005786:Gene Expression Regulation; D018392:Genomic Imprinting; D015698:Genomic Library; D007335:Insulin-Like Growth Factor II / Q000235:genetics*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000378:metabolism; D012097:Repressor Proteins / Q000378:metabolism*; D015183:Restriction Mapping; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection
C072278:GCFC2 protein, human; D011994:Recombinant Proteins; D012097:Repressor Proteins; D007335:Insulin-Like Growth Factor II