[Clinical significance of stress-protein content in peripheral blood lymphocytes of patients with myocardial infarction].
We studied the role of inducible stress-protein with molecular mass 70 kD (heat shock protein--HSP) in myocardial protection in ischemia. Content of HSP was measured in 25 patients with Q myocardial infarction (QMI) and 5 healthy controls. Western-blot was made on QMI day 1, 2, 7, 14 and 21, and once in the controls. Three types of HSP synthesis reaction manifested on QMI day 1. Type 1--HSP was absent in lymphocyte cytoplasm at 37 degrees C and after heat shock (HS); type 2--HSP accumulated after HS and was absent at 37 degrees C; type 3--HSP was present at 37 degrees C and after HS. Healthy subjects exhibited HSP after HS and the HSP synthesis was enhanced by HS. Type 1 was associated with favourable short-term prognosis of MI. Vast myocardial damage was accompanied with lowering synthesis with its activation at 37 degrees C by MI day 2. High synthesis of HSP on MI day 2-14 indicated moderate heart damage. Thus, HSP reactivity can be used as a factor of MI prognosis reflecting mobilization of intracellular defense proteins.
Ivashkin
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Zadorozhnaia
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D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000097:blood; D015153:Blotting, Western; D016022:Case-Control Studies; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000097:blood*; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009203:Myocardial Infarction / Q000097:blood* / Q000175:diagnosis*; D011237:Predictive Value of Tests; D011379:Prognosis; D013997:Time Factors
D015415:Biomarkers; D018840:HSP70 Heat-Shock Proteins