title: Relationship between tumour vascularity and circulating cancer cells in patients with colorectal carcinoma.
abstract: BACKGROUND
  Colorectal cancer vascularity correlates with risk of metastasis. Greater tumour vascularity may increase haematogenous dissemination by providing a larger vessel area for tumour cell invasion into the circulation. We assessed whether the prevalence of tumour cells in the circulation of colorectal carcinoma patients (CTC) increased with tumour vascularity.
  
  
  METHODS
  Pre-operative blood samples were assessed for circulating tumour cells using RT-PCR for carcinoembryonic antigen (CEA) and cytokeratin 20 (CK20) mRNA. Vessel count and volume were morphometrically assessed from tumour biopsies after vasculature staining.
  
  
  RESULTS
  Thirty-three colorectal cancer patients (M:F, 20:13; mean age 66 years, SD 11 years) were studied. One or more blood samples were RT-PCR positive for either CEA or CK20 mRNA or both, in 28 (85%) patients. There were no significant differences in the prevalence of RT-PCR positive patients between high and low tumour vascularity groups, or in tumour vessel counts or volume in RT-PCR positive compared with negative patients.
  
  
  CONCLUSIONS
  These results do not support vascularity related variation in access of tumour cells to the circulation as an explanation for the correlation between tumour vasculature and metastasis. Tumour vascularity and metastatic potential may be linked phenotypes rather than cause and effect.
authors: Department of Surgery, Imperial College School of Medicine, London SW10 9NH, UK.
authors: P
authors: P
authors: Mathur
authors: R Q
authors: RQ
authors: Wharton
authors: S K
authors: SK
authors: Jonas
authors: S
authors: S
authors: Saini
authors: T G
authors: TG
authors: Allen-Mersh
mesh_terms: D000368:Aged; D015179:Colorectal Neoplasms / Q000098:blood supply* / Q000473:pathology; D005260:Female; D006801:Humans; D008113:Liver Neoplasms / Q000556:secondary*; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D009360:Neoplastic Cells, Circulating