The gene for alternative oxidase-2 (AOX2) from Arabidopsis thaliana consists of five exons unlike other AOX genes and is transcribed at an early stage during germination.
We investigated the expressions of genes for alternative oxidase (AOX1a, AOX1b, AOX1c and AOX2) and genes for cytochrome c oxidase (COX5b and COX6b) during germination of Arabidopsis thaliana, and examined oxygen uptakes of the alternative respiration and the cytochrome respiration in imbibed Arabidopsis seeds. A Northern blot analysis showed that AOX2 mRNA has already accumulated in dry seeds and subsequently decreased, whereas accumulation ofAOX1a mRNA was less abundant from 0 hours to 48 hours after imbibition and then increased. The increase of the capacity of the alternative pathway appeared to be dependent on the expressions of both AOX2 and AOX1a. On the other hand, steady-state mRNA levels of COX5b and COX6b were gradually increased during germination, and the capacity of the cytochrome pathway was correlated with the increase of expressions of the COX genes. Antimycin A, the respiratory inhibitor, strongly increased the expression of AOX1a but had no effect on the expression of AOX2. A 5'RACE analysis showed that AOX2 consists of five exons, which is different from the case of most AOX genes identified so far. Analysis of subcellular localization of AOX2 using green fluorescent protein indicated that the AOX2 protein is imported into the mitochondria.
Saish
D
D
Laboratory of Plant Molecular Genetics, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan.
Nakazono
M
M
Lee
K H
KH
Tsutsumi
N
N
Akita
S
S
Hirai
A
A
D000595:Amino Acid Sequence; D000968:Antimycin A / Q000494:pharmacology; D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics*; D015152:Blotting, Northern; D003001:Cloning, Molecular; D005091:Exons; D049452:Green Fluorescent Proteins; D007438:Introns; D008164:Luminescent Proteins / Q000378:metabolism; D008856:Microscopy, Fluorescence; D024101:Mitochondrial Proteins; D008957:Models, Genetic; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000235:genetics*; D010100:Oxygen / Q000378:metabolism; D010940:Plant Proteins; D012333:RNA, Messenger / Q000378:metabolism; D013997:Time Factors; D000099188:Alternative Oxidase
D000968:Antimycin A; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins; D024101:Mitochondrial Proteins; D010088:Oxidoreductases; D010100:Oxygen; D010940:Plant Proteins; D012333:RNA, Messenger; D000099188:Alternative Oxidase