title: Somatic embryogenesis in Vigna radiata (L.) Wilczek.
abstract: Somatic embryogenesis was achieved from immature cotyledon derived callus of mungbean, V.radiata (L.) Wilczek in MS liquid medium. Embryogenic callus was induced on MS medium with NAA (5 mg/L). Differentiation of somatic embryos was observed when embryogenic callus was transferred to MS liquid medium containing 2,4-D (1.5 mg/L) and L-proline (50 mg/L). The torpedo shaped embryos were transferred to MS liquid medium with BAP and ABA (1 mg/L each) for maturation and germination. Fifty per cent of torpedo shaped embryos were converted into tiny plants (8-9 plants out of 17) after one week of culture. The germinated embryos were isolated and transferred to MS half strength basal (solid) medium for further development.
authors: Department of Biotechnology, School of Life Sciences, Bharathidasan University, Tiruchirappalli 620 024, India.
authors: S
authors: S
authors: Girija
authors: A
authors: A
authors: Ganapathi
authors: G
authors: G
authors: Ananthakrishnan
mesh_terms: D015084:2,4-Dichlorophenoxyacetic Acid / Q000494:pharmacology; D000383:Agriculture; D003470:Culture Media; D007887:Fabaceae / Q000187:drug effects / Q000196:embryology*; D007194:India; D009280:Naphthaleneacetic Acids / Q000494:pharmacology; D010946:Plants, Medicinal
chemical_list: D003470:Culture Media; D009280:Naphthaleneacetic Acids; D015084:2,4-Dichlorophenoxyacetic Acid