Cloning of two novel P450 cDNAs from German cockroaches, Blattella germanica (L.): CYP6K1 and CYP6J1.
Two novel P450 cDNAs, CYP6K1 and CYP6J1, were isolated from German cockroaches, Blattella germanica (L). Both CYP6K1 and CYP6J1 are typical microsomal P450s and their deduced amino acid sequences share a number of common characteristics with other members of the P450 superfamily. Both CYP6K1 and CYP6J1 showed the highest per cent identity (based on the deduced amino acid sequence) to CYP6L1 from B. germanica and CYP6H1, a putative ecdysone 20-hydroxylase from Locusta migratoria. Using a CYP6K1 probe, two mRNA signals (~2.5 and ~2.1 kb) were detected in all life stages. Both signals were just detectable in the eggs and became stronger in later instars. The strongest signals were detected in the fifth and sixth instars as well as in adults. These two bands were also detected in the abdomens and in the remainder of bodies of both male and female adults. Southern blots suggest the two mRNA bands detected in the Northern blot might be a result of alternative splicing. No signal could be detected at any life stage using the CYP6J1 probe, suggesting that CYP6J1 was expressed at a low level.
Wen
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Department of Entomology, Comstock Hall, Cornell University, Ithaca, NY 14853-0901, USA.
Scott
J G
JG
D000595:Amino Acid Sequence; D001483:Base Sequence; D020048:Blattellidae / Q000201:enzymology* / Q000235:genetics; D015152:Blotting, Northern / Q000379:methods; D015139:Blotting, Southern / Q000379:methods; D003001:Cloning, Molecular; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D018076:DNA, Complementary; D008969:Molecular Sequence Data
D018076:DNA, Complementary; D003577:Cytochrome P-450 Enzyme System; C434940:cytochrome P-450 CYP6J1 (German cockroach); C434939:cytochrome P-450 CYP6K1 (German cockroach)