title: Aberrant high expression of B lymphocyte chemokine (BLC/CXCL13) by C11b+CD11c+ dendritic cells in murine lupus and preferential chemotaxis of B1 cells towards BLC.
abstract: We observed here that the expression of B lymphocyte chemokine (BLC/CXCL13) was markedly enhanced in the thymus and kidney in aged (NZB x NZW)F1 (BWF1) mice developing lupus nephritis, but not in similarly aged NZB and NZW mice. BLC-positive cells were present in the cellular infiltrates in the target organs with a reticular pattern of staining. CD11b+CD11c+ dendritic cells were increased in the thymus and spleen in aged BWF1 mice and identified as the major cell source for BLC. CD4+ T cells as well as B cells were dramatically increased in the thymus in aged BWF1 mice, whereas no increase was observed in aged NZB and NZW mice. B1/B2 ratio in the thymus was significantly higher than those in the spleen and peripheral blood in aged BWF1 mice. Interestingly, BLC showed preferential chemotactic activity for B1 cells derived from several mouse strains, including nonautoimmune mice. Cell surface CXCR5 expression on B1 cells was significantly higher than that on B2 cells. Thus, aberrant high expression of BLC by myeloid dendritic cells in the target organs in aged BWF1 mice may play a pivotal role in breaking immune tolerance in the thymus and in recruiting autoantibody-producing B cells in the development of murine lupus.
authors: Department of Molecular Preventive Medicine, School of Medicine, The University of Tokyo, Tokyo 113-0033, Japan.
authors: S
authors: S
authors: Ishikawa
authors: T
authors: T
authors: Sato
authors: M
authors: M
authors: Abe
authors: S
authors: S
authors: Nagai
authors: N
authors: N
authors: Onai
authors: H
authors: H
authors: Yoneyama
authors: Y
authors: Y
authors: Zhang
authors: T
authors: T
authors: Suzuki
authors: S
authors: S
authors: Hashimoto
authors: T
authors: T
authors: Shirai
authors: M
authors: M
authors: Lipp
authors: K
authors: K
authors: Matsushima
mesh_terms: D000375:Aging; D000818:Animals; D016175:B-Lymphocyte Subsets / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D002478:Cells, Cultured; D054382:Chemokine CXCL13; D019743:Chemokines, CXC / Q000096:biosynthesis* / Q000235:genetics; D002634:Chemotaxis, Leukocyte; D003713:Dendritic Cells / Q000276:immunology*; D016167:Integrin alphaXbeta2 / Q000032:analysis*; D007668:Kidney / Q000276:immunology; D008099:Liver / Q000276:immunology; D008168:Lung / Q000276:immunology; D008181:Lupus Nephritis / Q000276:immunology*; D016177:Macrophage-1 Antigen / Q000032:analysis*; D051379:Mice; D012333:RNA, Messenger / Q000096:biosynthesis; D013950:Thymus Gland / Q000276:immunology; D015533:Transcriptional Activation
chemical_list: D054382:Chemokine CXCL13; D019743:Chemokines, CXC; C486386:Cxcl13 protein, mouse; D016167:Integrin alphaXbeta2; D016177:Macrophage-1 Antigen; D012333:RNA, Messenger