Characterization of human bone cells in culture.
Osteoblast-like cells isolated from human bone bioptic specimens were established in culture. Their osteoblast-like phenotype was studied by biochemical, histochemical and immunohistochemical methods and by electron microscopy examination. Third-passage cell cultures exhibited high level of alkaline phosphatase activity and the exposure to human parathyroid hormone produced an increase of intracellular cAMP. Cultured cells were immunoreactive for type I and type III collagen, osteonectin, and fibronectin; when ascorbic acid and beta-glycerophosphate were added, they synthesized a rich extracellular matrix. This characterization ensures the reliability of osteoblast-like cultures when they are used as experimental models.
Toesca
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Institute of Human Anatomy, Catholic University, Rome, Italy. ibiau@rm.unicatt.it
Pagnotta
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A
Specchia
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N
D000328:Adult; D000469:Alkaline Phosphatase / Q000032:analysis; D001205:Ascorbic Acid / Q000494:pharmacology; D001857:Bone Matrix / Q000378:metabolism; D002478:Cells, Cultured; D003094:Collagen / Q000032:analysis; D000242:Cyclic AMP / Q000378:metabolism; D005353:Fibronectins / Q000032:analysis; D005994:Glycerophosphates / Q000494:pharmacology; D006651:Histocytochemistry; D006801:Humans; D007150:Immunohistochemistry; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D015676:Osteonectin / Q000032:analysis; D010281:Parathyroid Hormone / Q000494:pharmacology
D005353:Fibronectins; D005994:Glycerophosphates; D015676:Osteonectin; D010281:Parathyroid Hormone; D003094:Collagen; D000242:Cyclic AMP; D000469:Alkaline Phosphatase; D001205:Ascorbic Acid; C031463:beta-glycerophosphoric acid