Atp11p and Atp12p are assembly factors for the F(1)-ATPase in human mitochondria.
Atp11p and Atp12p were first described as proteins required for assembly of the F(1) component of the mitochondrial ATP synthase in Saccharomyces cerevisiae (Ackerman, S. H., and Tzagoloff, A. (1990) Proc. Natl. Acad. Sci. U. S. A. 87, 4986-4990). Here we report the isolation of the cDNAs and the characterization of the human genes for Atp11p and Atp12p and show that the human proteins function like their yeast counterparts. Human ATP11 spans 24 kilobase pairs in 9 exons and maps to 1p32.3-p33, while ATP12 contains > or =8 exons and localizes to 17p11.2. Both genes are broadly conserved in eukaryotes and are expressed in a wide range of tissues, which suggests that Atp11p and Atp12p are essential housekeeping proteins of human cells. The information reported herein will be useful in the evaluation of patients with ascertained deficiencies in the ATP synthase, in which the underlying biochemical defect is unknown and may reside in a protein that influences the assembly of the enzyme.
Wang
Z G
ZG
Department of Surgery, Wayne State University School of Medicine, Detroit, Michigan 48201, USA.
White
P S
PS
Ackerman
S H
SH
D000595:Amino Acid Sequence; D018833:Chaperonins; D003001:Cloning, Molecular; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D006801:Humans; D008928:Mitochondria / Q000201:enzymology*; D024101:Mitochondrial Proteins; D025261:Mitochondrial Proton-Translocating ATPases; D018832:Molecular Chaperones; D008969:Molecular Sequence Data; D006180:Proton-Translocating ATPases / Q000737:chemistry* / Q000235:genetics / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins
C110382:ATP12 protein, S cerevisiae; D005656:Fungal Proteins; D024101:Mitochondrial Proteins; D018832:Molecular Chaperones; D029701:Saccharomyces cerevisiae Proteins; D018833:Chaperonins; C098664:ATP11 protein, S cerevisiae; C498318:ATPAF1 protein, human; C484671:ATPAF2 protein, human; D025261:Mitochondrial Proton-Translocating ATPases; D006180:Proton-Translocating ATPases