Effect of the universal base 3-nitropyrrole on the selectivity of neighboring natural bases.
[structure: see text] The effect of the universal base 3-nitropyrrole on the pairing selectivity of neighboring nucleosides has been determined for every combination of complementary and neighboring nucleosides. In a subset of cases the discriminatory ability of the neighboring nucleoside for its Watson-Crick complement is compromised. The results have implications for the hybridization of oligonucleotides that contain 3-nitropyrrole and suggest caution in the use of oligonucleotides that contain other universal bases.
Oliver
J S
JS
Department of Chemistry, Brown University, Providence, Rhode Island 02912, USA. john_oliver@brown.edu
Parker
K A
KA
Suggs
J W
JW
D020137:Base Pair Mismatch; D020029:Base Pairing; D009841:Oligonucleotides / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry*
D009841:Oligonucleotides; D011758:Pyrroles; C094911:3-nitropyrrole