title: Detection of malignant cells in pleural fluid or ascites by CD44v8-10/CD44v10 competitive RT-PCR.
abstract: BACKGROUND
  CD44 is a cell surface adhesion molecule which has been implicated in various biologic functions as lymphocyte homing and activation, cellular migration and extracellular matrix adhesion. Over-expression of CD44v8-10 has been found in several cancers and is considered to be associated with tumor progression and metastasis. Recently, a novel molecular method, CD44v8-10/CD44v10 competitive reverse transcription-polymerase chain reaction(RT-PCR) has been developed for detecting cancer cells over-expressing CD44v8-10.
  
  
  METHODS
  We analyzed from benign and malignant pleural effusion and ascites by CD44 competitive RT-PCR and compared to the conventional cytology.
  
  
  RESULTS
  The CD44 competitive RT-PCR analysis showed that all the 24 samples associated with benign disease presented a predominant expression of the CD44v10 transcript (v8-10/v10 ratio: 0.126-0.948), whereas 6 of 7 malignant pleural samples associated with cytology positive cancer expressed the CD44v8-10 transcript (v8-10/v10 ratio > 1.00).
  
  
  CONCLUSIONS
  These results indicate that CD44 competitive RT-PCR assay is a useful and adjunct to cytological examination in cancer diagnosis, especially in detecting exfoliated cancer cells in pleural effusion.
authors: Department of Internal Medicine, College of Medicine, Hanyang University.
authors: M J
authors: MJ
authors: Ahn
authors: Y H
authors: YH
authors: Noh
authors: H J
authors: HJ
authors: Yoon
authors: S C
authors: SC
authors: Yang
authors: J W
authors: JW
authors: Sohn
authors: J H
authors: JH
authors: Choi
authors: Y Y
authors: YY
authors: Lee
authors: I Y
authors: IY
authors: Choi
authors: I S
authors: IS
authors: Kim
authors: Y S
authors: YS
authors: Lee
authors: C K
authors: CK
authors: Park
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001201:Ascites / Q000276:immunology* / Q000473:pathology*; D001483:Base Sequence; D002289:Carcinoma, Non-Small-Cell Lung / Q000276:immunology / Q000473:pathology; D005260:Female; D005770:Gastrointestinal Neoplasms / Q000276:immunology / Q000473:pathology; D006801:Humans; D018960:Hyaluronan Receptors / Q000032:analysis*; D008175:Lung Neoplasms / Q000737:chemistry / Q000473:pathology; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016066:Pleural Effusion, Malignant / Q000737:chemistry* / Q000473:pathology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity
chemical_list: C117722:CD44v10 antigen; D018960:Hyaluronan Receptors