Neurodegeneration changes in primary central nervous system neurons transfected with the Alzheimer-associated neuronal thread protein gene.
The AD7c-NTP gene is over-expressed in brains with Alzheimer's disease (AD), and increased levels of the corresponding protein are detectable in cortical neurons, brain tissue extracts, cerebrospinal fluid, and urine beginning early in the course of AD neurodegeneration. In the present study, we utilized a novel method to transfect post-mitotic primary neuronal cell cultures, and demonstrated that over-expression of the AD7c-NTP gene causes cell death and neuritic sprouting, two prominent abnormalities associated with AD. These results provide further evidence that aberrantly increased AD7c-NTP expression may have a role in AD-type neurodegeneration. In addition, we demonstrate that primary post-mitotic neurons can be efficiently transfected with conventional recombinant plasmid DNA to evaluate the effects of gene over-expression in relevant in vitro models.
de la Monte
S M
SM
Rhode Island Hospital, Brown University Medical School, Providence 02903, USA. delamonte@hotmail.com
Wands
J R
JR
D000544:Alzheimer Disease / Q000235:genetics* / Q000473:pathology*; D000818:Animals; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D048429:Cell Size; D002470:Cell Survival; D002478:Cells, Cultured; D002531:Cerebellum / Q000473:pathology*; D015870:Gene Expression; D051847:Lithostathine; D009419:Nerve Tissue Proteins; D009474:Neurons / Q000378:metabolism* / Q000473:pathology*; D051381:Rats; D014162:Transfection
D002135:Calcium-Binding Proteins; D051847:Lithostathine; D009419:Nerve Tissue Proteins; C497616:Reg1a protein, rat