title: Two-dimensional structure of the native light-harvesting complex LH2 from Rubrivivax gelatinosus and of a truncated form.
abstract: The light-harvesting complex LH2 of Rubrivivax gelatinosus has an oligomeric structure built from alpha-beta heterodimers containing three bacteriochlorophylls and one carotenoid each. The alpha subunit (71 residues) presents a C-terminal hydrophobic extension (residues 51-71) which is prone to attack by an endogenous protease. This extension can also be cleaved by a mild thermolysin treatment, as demonstrated by electrophoresis and by matrix-assisted laser desorption-time of flight mass spectrometry. This cleavage does not affect the pigment binding sites as shown by absorption spectroscopy. Electron microscopy was used to investigate the structures of the native and thermolysin cleaved forms of the complexes. Two-dimensional crystals of the reconstituted complexes were examined after negative staining and cryomicroscopy. Projection maps at 10 A resolution were calculated, demonstrating the nonameric ring-like organization of alpha-beta subunits. The cleaved form presents the same structural features. We conclude that the LH2 complex is structurally homologous to the Rhodopseudomonas acidophila LH2. The hydrophobic C-terminal extension does not fold back in the membrane, but lays out on the periplasmic surface of the complex.
authors: Institut Curie, CNRS-UMR 168, Paris, France. jean-luc.ranck@curie.fr
authors: J
authors: J
authors: Ranck
authors: T
authors: T
authors: Ruiz
authors: G
authors: G
authors: Péhau-Arnaudet
authors: B
authors: B
authors: Arnoux
authors: F
authors: F
authors: Reiss-Husson
mesh_terms: D000595:Amino Acid Sequence; D003460:Crystallization; D003902:Detergents; D004591:Electrophoresis, Polyacrylamide Gel; D020689:Exopeptidases / Q000737:chemistry; D006217:Halobacterium / Q000737:chemistry*; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D015394:Molecular Structure; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry* / Q000648:ultrastructure; D012965:Sodium Chloride; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013820:Thermolysin / Q000737:chemistry
chemical_list: D003902:Detergents; D045322:Photosynthetic Reaction Center Complex Proteins; D012965:Sodium Chloride; D020689:Exopeptidases; D013820:Thermolysin