Enzyme biosensor for studying therapeutics of Alzheimer's disease.
An electrochemical method for the investigation and comparison of anti-Alzheimer medications that is based on the inhibition of the acetylcholinesterase is presented. The developed amperometric biosensor determines the in-vitro inhibition of the acetylcholinesterase that is co-immobilized with choline oxidase on the working electrode surface of a three-electrode system using gel entrapment. The sensor has been applied to determine the IC50 values of two known and one newly developed Alzheimer remedy. A simultaneous measurement with the photometric standard method shows the applicability of our method for fast drug screening.
Lenigk
R
R
Department of Chemistry, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, PR China. chlenigk@ust.hk
Lam
E
E
Lai
A
A
Wang
H
H
Han
Y
Y
Carlier
P
P
Renneberg
R
R
D000110:Acetylcholinesterase; D000429:Alcohol Oxidoreductases; D000544:Alzheimer Disease / Q000188:drug therapy*; D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D002417:Cattle; D002800:Cholinesterase Inhibitors / Q000494:pharmacology; D004353:Drug Evaluation, Preclinical / Q000379:methods; D004800:Enzymes, Immobilized; D006801:Humans; D066298:In Vitro Techniques; D051381:Rats
D002800:Cholinesterase Inhibitors; D004800:Enzymes, Immobilized; D000429:Alcohol Oxidoreductases; C019888:choline oxidase; D000110:Acetylcholinesterase