Mouse model for thoracic congenital scoliosis.
This study sought to produce a dose-response curve for acute and chronic maternal carbon monoxide (CO) exposure versus vertebral anomalies in mouse offspring and to determine the critical day of exposure. In Part I, pregnant CD-1 mice were exposed to an acute dose of CO at 9 days of gestation. A positive dose-response relationship of acute maternal CO exposure and vertebral anomalies in the offspring was produced. In Part II, pregnant females were exposed to chronic CO for the first 11 days of gestation. Chronic exposure to CO did not produce significant vertebral anomalies. In Part III, pregnant females were exposed to an acute dose of 600 ppm of CO at gestation day 8, 9, or 10. Day 9 in this mouse breed is the critical day for maternal exposure to CO. The detected anomalies were predominately in the thoracic spine.
Farley
F A
FA
Section of Orthopaedic Surgery , University of Michigan Medical Center, Ann Arbor, Michigan 48109-0328, USA. fafarley@umich.edu
Loder
R T
RT
Nolan
B T
BT
Dillon
M T
MT
Frankenburg
E P
EP
Kaciroti
N A
NA
Miller
J D
JD
Goldstein
S A
SA
Hensinger
R N
RN
D000208:Acute Disease; D000818:Animals; D002249:Carbon Monoxide Poisoning / Q000097:blood / Q000150:complications*; D002908:Chronic Disease; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D005260:Female; D005865:Gestational Age; D018811:Maternal Exposure / Q000009:adverse effects*; D051379:Mice; D008815:Mice, Inbred Strains; D015588:Observer Variation; D011247:Pregnancy; D011859:Radiography; D012600:Scoliosis / Q000139:chemically induced* / Q000151:congenital* / Q000000981:diagnostic imaging; D013904:Thoracic Vertebrae; D013997:Time Factors