[A modification of the in ovo-method for the determination of the antihemorrhagic activity of plant extracts].
Up to now there has been only a method available for testing the antihaemorrhagic activity of plant extracts, in which chicken embryos had to be transferred to a special container on the fifth day of incubation. Furthermore, the test could not be conducted on the extraembryonal circulatory system until two days later. In the test method described here the embryos remain inside the egg. After removal of the eggshell through the air cell and removal of the inner shell membrane, direct access to the extraembryonal circulatory system is possible, minimising external influences on the test system. This represents a dramatic simplification in technique and permits testing already on the fourth day of incubation. The application of this method to determine the antihaemorrhagic properties of samples has the advantage of permitting the simple and quick production of haemorrhages by snake venom on the yolk sac circulatory system.
Glünder
G
G
Chemisches Institut, Tierärztlichen Hochschule Hannover.
Jantzen
K
K
Habermehl
G G
GG
D000818:Animals; D002642:Chick Embryo / Q000098:blood supply / Q000187:drug effects*; D004528:Egg Shell; D006470:Hemorrhage / Q000139:chemically induced / Q000517:prevention & control*; D006490:Hemostatics / Q000494:pharmacology* / Q000627:therapeutic use; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D012910:Snake Venoms / Q000633:toxicity
D006490:Hemostatics; D010936:Plant Extracts; D012910:Snake Venoms