Antenatal screening for hepatitis B and antibodies to Toxoplasma gondii and rubella virus: evaluation of two commercial immunoassay systems.
A comparative evaluation of the Abbott AxSYM and DPC Immulite random-access analyzers was performed using 497 prospectively collected serum samples. These samples were sent to the laboratory for routine antenatal screening for hepatitis B surface antigen and immunoglobulin G (IgG) and IgM antibodies to Toxoplasma gondii and rubella virus. The overall agreement between the two assay systems ranged from 97.4 to 100%. After discrepancy analysis, the outcome in terms of sensitivity and specificity varied from 98.2 to 100% for all but one of the assays tested. The AxSYM rubella virus IgG assay tended to report protective or indeterminate antibody levels in 1% of the samples. This shortcoming might be overcome by raising the cutoff of the microparticle enzyme immunoassay system.
Diepersloot
R J
RJ
Laboratory for Medical Microbiology, Diakonessen Hospital, Utrecht, The Netherlands. Rdiepersloot@diakhuis.nl
Dunnewold-Hoekstra
H
H
Kruit-Den Hollander
J
J
Vlaspolder
F
F
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood* / Q000276:immunology; D000914:Antibodies, Viral / Q000097:blood* / Q000276:immunology; D005260:Female; D006509:Hepatitis B / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D006514:Hepatitis B Surface Antigens / Q000097:blood*; D006515:Hepatitis B virus / Q000276:immunology / Q000302:isolation & purification; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D011247:Pregnancy; D011296:Prenatal Diagnosis / Q000379:methods*; D011446:Prospective Studies; D011933:Reagent Kits, Diagnostic; D012409:Rubella / Q000175:diagnosis* / Q000276:immunology; D014122:Toxoplasma / Q000276:immunology; D014123:Toxoplasmosis / Q000097:blood / Q000175:diagnosis* / Q000276:immunology
D000913:Antibodies, Protozoan; D000914:Antibodies, Viral; D006514:Hepatitis B Surface Antigens; D011933:Reagent Kits, Diagnostic