Degradation of lignin in wheat straw during growth of the oyster mushroom (Pleurotus ostreatus) using off-line thermochemolysis with tetramethylammonium hydroxide and solid-state (13)C NMR.
The oyster mushroom (Pleurotus ostreatus) is widely cultivated on wheat straw (Triticum aestivum); however, there is a need to better understand the relationship between the chemical composition of the compost and mushroom growth. Wheat straw was degraded over a period of 63 days by P. ostreatus during which time it was sampled at weekly intervals. Off-line thermochemolysis with tetramethylammonium hydroxide and solid-state (13)C NMR were then used in the molecular characterization of the undegraded wheat straw and the degraded samples. The degraded wheat straw samples had a lower proportion of syringyl- to guaiacyl-derived moieties and cinnamyl- to guaiacyl-derived moieties than the undegraded control. There were increases in both guaiacyl and syringyl acid to aldehyde ratios with composting time, which showed that side-chain oxidation has been mediated by P. ostreatus. The (13)C NMR spectra confirmed the increase in carboxyl content but indicated that the overall lignin and methoxyl contents remained relatively constant, although some nonsystematic variations were observed. The spectra also showed a decrease in amorphous noncellulosic polysaccharides in relation to the crystalline cellulose upon degradation.
Vane
C H
CH
Department of Fossil Fuels and Environmental Geochemistry (Postgraduate Institute), NRG, Drummond Building, University of Newcastle upon Tyne, Newcastle upon Tyne NE1 7RU, United Kingdom.
Martin
S C
SC
Snape
C E
CE
Abbott
G D
GD
D002247:Carbon Isotopes; D005731:Ganglionic Stimulants; D008031:Lignin / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D010084:Oxidation-Reduction; D020076:Pleurotus / Q000378:metabolism*; D000644:Quaternary Ammonium Compounds; D014908:Triticum / Q000737:chemistry / Q000378:metabolism*
D002247:Carbon Isotopes; D005731:Ganglionic Stimulants; D000644:Quaternary Ammonium Compounds; D008031:Lignin; C027917:tetramethylammonium