title: Induction of interleukin-8 in human retinal pigment epithelial cells after denuding injury.
abstract: OBJECTIVE
  To determine interleukin 8 (IL-8) and monocyte chemoattractant protein 1 (MCP-1) expression in response to mechanical injury in human retinal pigment epithelial (HRPE) cells.
  
  
  METHODS
  Enzyme linked immunosorbent assay (ELISA) was performed to determine IL-8 and MCP-1 secretion by HRPE cells after mechanical denudation. IL-8 and MCP-1 mRNA expression by HRPE cells was assessed using semiquantitative RT-PCR. The effects of immunosuppressive drugs, dexamethasone (DEX) and cyclosporin A (CSA), as well as immunosuppressive cytokines, interleukin 4 (IL-4), interleukin 10 (IL-10), and interleukin 13 (IL-13), on chemokine expression in HRPE cells after denuding injury were analysed.
  
  
  RESULTS
  Mechanical injury induced HRPE IL-8 mRNA and IL-8 secretion. Although MCP-1 mRNA was enhanced slightly after denuding injury, MCP-1 secretion was not increased. DEX and CSA inhibited HRPE chemokine expression after injury. IL-4 and IL-13 enhanced IL-8 and MCP-1 production by HRPE cells after injury while IL-10 had no effect.
  
  
  CONCLUSIONS
  These results suggest that IL-8 may be involved in retinal inflammatory responses to injury and that DEX and/or CSA treatment may help control the inflammatory components of retinal diseases such as proliferative vitreoretinopathy.
authors: Department of Ophthalmology, University of Michigan, Ann Arbor, MI 48105, USA.
authors: A
authors: A
authors: Yoshida
authors: S G
authors: SG
authors: Elner
authors: Z M
authors: ZM
authors: Bian
authors: V M
authors: VM
authors: Elner
mesh_terms: D000328:Adult; D000704:Analysis of Variance; D018932:Chemokine CCL2 / Q000032:analysis* / Q000235:genetics; D016572:Cyclosporine / Q000494:pharmacology; D003907:Dexamethasone / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D004847:Epithelial Cells / Q000276:immunology; D006801:Humans; D007150:Immunohistochemistry; D007166:Immunosuppressive Agents / Q000494:pharmacology; D016753:Interleukin-10 / Q000494:pharmacology; D018793:Interleukin-13 / Q000494:pharmacology; D015847:Interleukin-4 / Q000494:pharmacology; D016209:Interleukin-8 / Q000032:analysis* / Q000235:genetics; D008875:Middle Aged; D010857:Pigment Epithelium of Eye / Q000276:immunology* / Q000293:injuries*; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018630:Vitreoretinopathy, Proliferative / Q000188:drug therapy
chemical_list: D018932:Chemokine CCL2; D007166:Immunosuppressive Agents; D018793:Interleukin-13; D016209:Interleukin-8; D012333:RNA, Messenger; D016753:Interleukin-10; D015847:Interleukin-4; D003907:Dexamethasone; D016572:Cyclosporine