Biochemical mechanisms of resistance in strains of Oryzaephilus surinamensis (Coleoptera: Silvanidae) resistant to malathion and chlorpyrifos-methyl.
The acetylcholinesterase, carboxylesterase, and cytochrome P450 monooxygenase activities of three strains of Oryzaephilus srinamensis (L.) were examined to better understand biochemical mechanisms of resistance. The three strains were VOS49 and VOSCM, selected for resistance to malathion and chlorpyrifos-methyl, respectively, and VOS48, a standard susceptible strain. Cross-resistance to malathion and chlorpyrifos-methyl was confirmed in VOS49 and VOSCM. Acetylcholinesterase activity was not correlated to resistance among these strains. VOS49 and VOSCM showed elevated levels of carboxylesterase activity based on p-nitrophenylacetate, alpha-naphthyl acetate, or beta-naphthyl acetate substrates. PAGE zymograms showed major differences in caboxylesterase isozyme banding among strains. VOSCM had one strongly staining isozyme band. A band having the same Rf-value was very faint in VOS48. The VOS49 carboxylesterase banding pattern was different from both VOSCM and VOS48. Cytochrome P450 monooxygenase activity was based on cytochrome P450 content, aldrin epoxidase activity, and oxidation of organophosphate insecticides, all elevated in resistant strains. The monooxygenase activity varied with insecticide substrate and resistant strain, suggesting specific cytochromes P450 may exist for different insecticides. The monooxygenase activity of the VOS49 strain was much higher with malathion than chlorpyrifos-methyl as substrates, whereas VOSCM monooxygenase activity was higher with malathion than chlorpyrifos-methyl as substrates. Results are discussed in the context of resistance mechanisms to organophosphate insecticides in O. surinamensis.
Lee
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Plant Protection Research Unit, WRRC, USDA-ARS, Albany, CA 94710, USA.
Lees
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D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D043182:Carboxylesterase; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism; D004390:Chlorpyrifos / Q000031:analogs & derivatives* / Q000378:metabolism* / Q000494:pharmacology; D002800:Cholinesterase Inhibitors / Q000378:metabolism* / Q000494:pharmacology; D001517:Coleoptera / Q000187:drug effects / Q000201:enzymology*; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D005278:Fenitrothion / Q000378:metabolism / Q000494:pharmacology; D007305:Insecticide Resistance; D007306:Insecticides / Q000378:metabolism* / Q000494:pharmacology; D007928:Lethal Dose 50; D008294:Malathion / Q000378:metabolism* / Q000494:pharmacology
D002800:Cholinesterase Inhibitors; D007306:Insecticides; D003577:Cytochrome P-450 Enzyme System; D002265:Carboxylic Ester Hydrolases; D043182:Carboxylesterase; D000110:Acetylcholinesterase; D004390:Chlorpyrifos; C007031:chlorpyrifos-methyl; D008294:Malathion; D005278:Fenitrothion