title: Immunocompetent astrocytes and microglia display major differences in the processing of the invariant chain and in the expression of active cathepsin L and cathepsin S.
abstract: The role of astrocytes and microglia as antigen-presenting cells in the brain is still controversial. In this study we have analyzed and compared aspects of the molecular machinery that underlies MHC class II trafficking in immunocompetent astrocytes and microglia. We show that IFN-gamma-stimulated microglia possess active cathepsin L and cathepsin S, and efficiently degrade the invariant chain, unlike IFN-gamma-stimulated astrocytes that express cathepsin L but not cathepsin S. The lack of cathepsin S proves to be dramatic for the antigen-presentation capacity of astrocytes, which is nearly abolished when these cells are stimulated by a combination of IFN-gamma and TNF-alpha. TNF-alpha indeed decreases cathepsin L activity as we show here, leading to alterations in invariant chain processing, and hence in MHC class II trafficking in astrocytes. Cystatin C inhibits cathepsin L activity in astrocytes, but does not regulate cathepsin L and cathepsin S activity in microglia. We therefore identify cathepsin L and cathepsin S as key components in the regulation of the immune potential of astrocytes and microglia, and provide evidence for a cell-specific regulation exerted by IFN-gamma and TNF-alpha on the expression and activity of cathepsins.
authors: Department of Neurobiology, Interdisziplinäres Zentrum für Neurowissenschaften, University of Heidelberg, Heidelberg, Germany.
authors: O
authors: O
authors: Gresser
authors: E
authors: E
authors: Weber
authors: A
authors: A
authors: Hellwig
authors: S
authors: S
authors: Riese
authors: A
authors: A
authors: Régnier-Vigouroux
mesh_terms: D017951:Antigen Presentation / Q000187:drug effects / Q000276:immunology*; D000944:Antigens, Differentiation, B-Lymphocyte / Q000276:immunology*; D001253:Astrocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D056668:Cathepsin L; D002403:Cathepsins / Q000276:immunology*; D002478:Cells, Cultured; D003546:Cysteine Endopeptidases; D010450:Endopeptidases; D011992:Endosomes / Q000648:ultrastructure; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D006801:Humans; D007121:Immunocompetence; D007371:Interferon-gamma / Q000276:immunology / Q000494:pharmacology; D008247:Lysosomes / Q000648:ultrastructure; D017628:Microglia / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology / Q000494:pharmacology
chemical_list: D000944:Antigens, Differentiation, B-Lymphocyte; D000949:Histocompatibility Antigens Class II; D014409:Tumor Necrosis Factor-alpha; C045084:invariant chain; D007371:Interferon-gamma; D002403:Cathepsins; D010450:Endopeptidases; D003546:Cysteine Endopeptidases; C534281:CTSL protein, human; D056668:Cathepsin L; C028217:cathepsin S