title: The frequency of anti-C + anti-G in the absence of anti-D in alloimmunized pregnancies.
abstract: Anti-D+C are often initially identified in sera from alloimmunized women. Anti-G may be present in these samples, mimicking anti-D+C, and therefore the differentiation of anti-D, -C and -G may be important. Sera from 27 alloimmunized women, initially identified as containing anti-D + anti-C, were analysed by adsorption/elution studies in the presence of polyethylene glycol (PEG) using R(0)r (D+C-G+) and r'r(D-C+G+) red blood cells (RBC). Additionally, 15/27 samples were tested by adsorption in the presence of PEG and subsequently warm elution, using rGr (D-C-G+) RBC. Anti-G + anti-C, without anti-D, were identified in 4/27 samples (14.8%) and none of the newborn children needed postpartum treatment. The combination of D+G, D+C and D+C+G antibodies occurred in 25.9%, 11.1% and 48.1% of the women, respectively. Overall, anti-G was detected in 24/27 samples (88.9%). Pregnant women shown to have anti-G+C but not anti-D should receive Rh immune globulin. Additionally, the finding of apparent anti-D+C during pregnancy in D-negative spouses may lead to paternity testing and therefore a correct antibody identification is necessary.
authors: Transfusion Medicine & Clinical Immunology, University Hospital, Linköping, Sweden. Miodrag.Palfi@skane.se
authors: M
authors: M
authors: Palfi
authors: C
authors: C
authors: Gunnarsson
mesh_terms: D000017:ABO Blood-Group System / Q000276:immunology*; D000327:Adsorption; D001787:Blood Group Incompatibility / Q000276:immunology* / Q000628:therapy; D017218:Cordocentesis; D004899:Erythroblastosis, Fetal / Q000276:immunology; D005260:Female; D005865:Gestational Age; D006801:Humans; D007518:Isoantibodies / Q000097:blood; D011092:Polyethylene Glycols; D011247:Pregnancy; D012203:Rh Isoimmunization; D018029:Rho(D) Immune Globulin / Q000627:therapeutic use
chemical_list: D000017:ABO Blood-Group System; D007518:Isoantibodies; D018029:Rho(D) Immune Globulin; D011092:Polyethylene Glycols