title: Modified version of pACT-2 that simplifies cloning with NdeI.
authors: Dept. of Cell Biology and Human Anatomy, 1 Shields Ave., University of California, Davis, CA 95616-8643, USA. jfhess@ucdavis.edu
authors: J F
authors: JF
authors: Hess
authors: J T
authors: JT
authors: Casselman
authors: P
authors: P
authors: FitzGerald
mesh_terms: D000818:Animals; D002417:Cattle; D003001:Cloning, Molecular / Q000379:methods*; D018076:DNA, Complementary / Q000235:genetics; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D005136:Eye Proteins / Q000235:genetics* / Q000378:metabolism; D005822:Genetic Vectors / Q000235:genetics*; D006801:Humans; D007381:Intermediate Filament Proteins / Q000235:genetics* / Q000378:metabolism; D016297:Mutagenesis, Site-Directed; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D020798:Two-Hybrid System Techniques; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D005136:Eye Proteins; D007381:Intermediate Filament Proteins; D011993:Recombinant Fusion Proteins; C079098:filensin; C037106:endodeoxyribonuclease NdeI; D015252:Deoxyribonucleases, Type II Site-Specific; D001616:beta-Galactosidase