title: Dysfunctional Epstein-Barr virus (EBV)-specific CD8(+) T lymphocytes and increased EBV load in HIV-1 infected individuals progressing to AIDS-related non-Hodgkin lymphoma.
abstract: Acquired immunodeficiency syndrome-related non-Hodgkin lymphomas (AIDS-NHL) are thought to arise because of loss of Epstein-Barr Virus (EBV)-specific cellular immunity. Here, an investigation was done to determine whether cellular immunity to EBV is lost because of physical loss or dysfunction of EBV-specific cytotoxic T cells. Data on EBV-specific cellular immunity were correlated with EBV load. For comparison, individuals who progressed to AIDS with opportunistic infections (AIDS-OI) and long-term asymptomatics (LTAs) were studied. The number of virus-specific T cells was detected using tetrameric HLA-EBV-peptide complexes; function of these EBV-specific T cells was determined using the interferon-gamma (IFN-gamma) Elispot assay. It was observed that EBV-specific CD8(+) T cells were present in normal numbers in human immunodeficiency virus (HIV)-infected individuals. However, their functional capacity was decreased compared with HIV(-) individuals. In AIDS-NHL patients, EBV-specific T cells were not physically lost in the course of HIV-1 infection but showed progressive loss of their capability to produce IFN-gamma in response to EBV peptides. This loss of function correlated with lower CD4(+) T-cell numbers and was accompanied by increasing EBV load. In HIV-1-infected LTA individuals, in whom CD4(+) T-cell numbers were maintained, and progressors to AIDS-OI, IFN-gamma-producing EBV-specific T cells were stable and EBV load remained stable or decreased in the course of HIV infection, suggestive of immune control. Our data indicate that functional loss of EBV-specific CD8(+) T cells with a concomitant increase in EBV load may play a role in the pathogenesis of AIDS-NHL.
authors: Department of Hematology, University of Amsterdam, Amsterdam, The Netherlands. d_van_baarle@clb.nl
authors: D
authors: D
authors: van Baarle
authors: E
authors: E
authors: Hovenkamp
authors: M F
authors: MF
authors: Callan
authors: K C
authors: KC
authors: Wolthers
authors: S
authors: S
authors: Kostense
authors: L C
authors: LC
authors: Tan
authors: H G
authors: HG
authors: Niesters
authors: A D
authors: AD
authors: Osterhaus
authors: A J
authors: AJ
authors: McMichael
authors: M H
authors: MH
authors: van Oers
authors: F
authors: F
authors: Miedema
mesh_terms: D017088:AIDS-Related Opportunistic Infections; D000328:Adult; D000956:Antigens, Viral / Q000097:blood / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000473:pathology* / Q000821:virology; D004279:DNA, Viral / Q000097:blood; D015658:HIV Infections / Q000097:blood / Q000276:immunology* / Q000821:virology; D020276:HIV Long-Term Survivors; D015497:HIV-1; D004854:Herpesvirus 4, Human / Q000276:immunology* / Q000472:pathogenicity; D006801:Humans; D016483:Lymphoma, AIDS-Related / Q000097:blood / Q000209:etiology / Q000821:virology*; D008297:Male; D008875:Middle Aged; D013997:Time Factors; D019562:Viral Load
chemical_list: D000956:Antigens, Viral; D004279:DNA, Viral