title: Study of the mode of action of endopolygalacturonase from Fusarium moniliforme.
abstract: One endopolygalacturonase from Fusarium moniliforme was purified from the culture broth of a transformed strain of Saccharomyces cerevisiae. Its kinetic parameters and mode of action were studied on galacturonic acid oligomers and homogalacturonan. The dimer was not a substrate for the enzyme. The enzyme was shown to follow Michaelis-Menten behaviour towards the other substrates tested. Affinity and maximum rate of hydrolysis increased with increasing chain length, up to the hexamer or heptamer, for which V(max) was in the same range as with homogalacturonan. The enzyme was demonstrated to have a multi-chain attack mode of action and its active site included five subsites ranging from -3 to +2. The final products of hydrolysis of homogalacturonan were the monomer and the dimer of galacturonic acid.
authors: Unité de Recherche sur les Polysaccharides, leurs Organisations et Interactions, INRA, Nantes, France. bonnin@nantes.inra.fr
authors: E
authors: E
authors: Bonnin
authors: A
authors: A
authors: Le Goff
authors: R
authors: R
authors: Körner
authors: G W
authors: GW
authors: Van Alebeek
authors: T M
authors: TM
authors: Christensen
authors: A G
authors: AG
authors: Voragen
authors: P
authors: P
authors: Roepstorff
authors: C
authors: C
authors: Caprari
authors: J
authors: J
authors: Thibault
mesh_terms: D001665:Binding Sites; D005670:Fusarium / Q000201:enzymology*; D006603:Hexuronic Acids / Q000378:metabolism; D007700:Kinetics; D008956:Models, Chemical; D010368:Pectins / Q000378:metabolism; D011096:Polygalacturonase / Q000302:isolation & purification / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D006603:Hexuronic Acids; C007819:galacturonic acid; D010368:Pectins; D011096:Polygalacturonase; C003181:polygalacturonic acid