Avidity analysis of the human immune response to a chitin binding protein of Toxoplasma gondii.
A 30-33 kDa electroeluted fraction of T. gondii tachyzoites improved discrimination between acute and chronic phase sera when used instead of the whole tachyzoite extract in an avidity-ELISA. In order to identify the components of these fractions, crude tachyzoite antigen was fractionated by anionic exchange chromatography. The 30-33 kDa antigen cluster eluted in the not-bound fraction could account for a large proportion of the antibody response against the 30-33 kDa electroeluted fraction. According to the N-terminal sequence data, this antigen fraction is composed mainly of SAG1 and another protein with high homology to chitin binding proteins from plants.
Villavedra
M
M
Cátedra de Inmunología, Facultad de Química - Facultad de Ciencias. Universidad de la República. Av. Alfredo Navarro 3051, Montevideo, Uruguay. mvilla@bilbo.edu.uy
Battistoni
J J
JJ
Rossi
S
S
Carol
H
H
Nieto
A
A
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood / Q000276:immunology*; D000915:Antibody Affinity / Q000276:immunology; D000918:Antibody Specificity; D000953:Antigens, Protozoan / Q000276:immunology* / Q000302:isolation & purification; D002686:Chitin / Q000378:metabolism; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D015800:Protozoan Proteins / Q000276:immunology* / Q000302:isolation & purification; D020539:Sequence Analysis, Protein; D014122:Toxoplasma / Q000276:immunology*; D014123:Toxoplasmosis / Q000175:diagnosis / Q000276:immunology*
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D007074:Immunoglobulin G; D015800:Protozoan Proteins; C047168:SAG1 antigen, Toxoplasma; D002686:Chitin