[Lung autofluorescence. Preliminary study of two systems without laser illumination or photosensitization].
When illuminated with a certain wavelength, lung tissue emits a weak fluorescence. The fluorescence from normal tissue is different from that from diseased tissue. This technique can thus be used for early diagnosis of precancerous lesions. Technical manipulations are required to amplify the fluorescence signal. Numerous studies have used the LIFE (Lung Imaging Fluorescent Endoscope) to study lung diseases. This system of detection uses a laser helium-cadmium excitation source; the principal photosensitizing agents are hematoporphyrin derivatives. The cost, bulkiness and secondary effects have limited diffusion of this type of photodynamic diagnostic system. We have used 2 systems (SAFE 1000 Pentax and D-LIGHT Storz) successively in a prospective study to analyze autofluorescence of lung tissue without laser illumination or photosensitization. Fifty-four patients were selected for their risk factors. We were able to detect one case of severe dysplasia but did not identify any case of carcinoma in situ. Among 18 cases, 16 were evident with white light. Two were detected with autofluorescence used to orient biopsies while white light had only revealed a common inflammatory aspect. Thirty-nine low-grade lesions (metaplasia, dysplasia) were found. The systems are easy to use. Lower cost would allow more widespread use of these systems currently reserved for risk populations. These techniques broaden the scope of interventional endoscopy.
Homasson
J P
JP
Centre Hospitalier Spécialisé en Pneumologie, 24, rue Albert Thuret, 94669 Chevilly-Larue.
Capron
F
F
Angebault
M
M
Nguyen Bich
N
N
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001706:Biopsy; D001999:Bronchoscopy / Q000191:economics / Q000379:methods*; D004867:Equipment Design; D005260:Female; D006801:Humans; D007834:Lasers; D008171:Lung Diseases / Q000175:diagnosis* / Q000209:etiology; D008175:Lung Neoplasms / Q000175:diagnosis* / Q000209:etiology; D008297:Male; D008875:Middle Aged; D017319:Photosensitizing Agents; D011230:Precancerous Conditions / Q000175:diagnosis* / Q000209:etiology; D011446:Prospective Studies; D012680:Sensitivity and Specificity; D012907:Smoking / Q000009:adverse effects; D013050:Spectrometry, Fluorescence / Q000191:economics / Q000295:instrumentation / Q000379:methods*
D017319:Photosensitizing Agents