Peroxidase reaction as a parameter for discrimination of Streptococcus mutans and Streptococcus sobrinus.
425 strains of mutans streptococci and 12 reference strains were investigated by membrane fatty acid spectra (MFAS) and peroxidase reaction (PR) after aerobic and anaerobic incubation. 423 strains were identified as Streptococcus mutans. The remaining 2 strains were identified as Streptococcus sobrinus. The PR of 29 strains was doubtful; immediately after anaerobic incubation a negative PR changed into a slightly positive PR. To test the diagnostic value of PR the strains were additionally investigated by means of species-specific polymerase chain reactions (PCR). The species-specific PCRs were developed on the basis of the respective genes of 16S rRNA of the pathogens S. mutans and S. sobrinus. Specificity and sensitivity were tested on reference strains (n = 17) and negative control strains (n = 39). The results of this investigation showed that an anaerobic incubation regime could lead to false-positive (S. mutans) or false-negative (S. sobrinus) PR. The 425 MS strains were classified as either S. mutans (n = 420) or S. sobrinus (n = 5). The findings on the reference strains required a reclassification of S. mutans V 100 into S. sobrinus V 100. Summarising, it is possible now to differentiate strains of mutans streptococci by MFAS and PR after aerobic incubation.
Rupf
S
S
Department of Operative Dentistry and Periodontology, School of Medicine, University of Leipzig, Germany.
Merte
K
K
Eschrich
K
K
Stösser
L
L
Kneist
S
S
D000332:Aerobiosis; D000693:Anaerobiosis; D002648:Child; D002849:Chromatography, Gas; D003470:Culture Media; D003773:Dental Plaque / Q000382:microbiology; D004587:Electrophoresis, Agar Gel; D005188:False Negative Reactions; D005189:False Positive Reactions; D005227:Fatty Acids / Q000032:analysis; D006735:Horseradish Peroxidase; D006801:Humans; D008563:Membrane Lipids / Q000032:analysis; D016133:Polymerase Chain Reaction; D011237:Predictive Value of Tests; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012680:Sensitivity and Specificity; D013045:Species Specificity; D013295:Streptococcus mutans / Q000145:classification* / Q000235:genetics; D016986:Streptococcus sobrinus / Q000145:classification* / Q000235:genetics
D003470:Culture Media; D005227:Fatty Acids; D008563:Membrane Lipids; D012336:RNA, Ribosomal, 16S; D006735:Horseradish Peroxidase