[Clinical significance of differential display cDNA fragment MRG98.2 expression in hepatocellular carcinoma].
OBJECTIVE
To search for the gene related to hepatocellular carcinoma (HCC), and to study its potential significance in liver oncogenesis.


METHODS
The difference in mRNA expression among HCC, nontumorous liver tissue, and normal liver tissue was investigated by differential display technique. Ten hepatic tissues of hepatoma versus surrounding noncancerous liver tissues were examined with MRG98.2 as a probe in Dot blot analysis. Expression of vascular endothelial growth factor (VEGF) mRNA in above-mentioned samples was examined with reverse transcription-polymerease chain reaction (RT-PCR).


RESULTS
Differential display cDNA fragment MRG98.2 was isolated from hepatoma samples. Dot blot analysis showed that MRG98.2 was expressed in 7 cases of HCC samples (7/10) but only in 2 of the surrounding noncancerous hepatic tissues (2/10). The expression of VEGF mRNA was upregulated in 6/7 of HCC samples expressed MRG98.2.


CONCLUSIONS
MRG98.2 may be a gene related to HCC. Its expression in HCC correlates with VEGF mRNA. MRG98.2 expression is helpful in predicting a tendency toward invasion and metastasis of HCC or a poor prognosis.
Shen
W
W
Department of Digestive Diseases, Second Affiliated Hospital of Chongqing University of Medical Sciences, Chongqing 400010, China.
Wu
X
X
D006528:Carcinoma, Hepatocellular / Q000235:genetics*; D018076:DNA, Complementary / Q000032:analysis*; D016228:Endothelial Growth Factors / Q000235:genetics; D020869:Gene Expression Profiling; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics*; D008222:Lymphokines / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
D018076:DNA, Complementary; D016228:Endothelial Growth Factors; D008222:Lymphokines; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors