title: The potential of bone marrow-derived cells to differentiate to glomerular mesangial cells.
abstract: Bone marrow stem cells (BMC) develop into hematopoietic and mesenchymal lineages but have not been known to differentiate into glomerular cells. To investigate whether such differentiation is possible, a search was made for donor glomerular cells in lethally irradiated C57BL/6j (B6) mice given transplants of BMC from syngeneic mice transgenic for green fluorescence protein (GFP) ([GFP-->B6] mice). After the recipients of donor BMC manifested GFP-positive cells in their glomeruli, the numbers of such cells increased markedly, in a time-dependent manner, from 2 wk to 24 wk after bone marrow transplantation. Immunohistochemical analyses revealed that most GFP-positive cells in the glomeruli were neither macrophages nor T cells. With the use of a laser-scanning confocal microscope, GFP-positive cells were observed within the mesangium of [GFP-->B6] mice. Furthermore, indirect immunofluorescence assays demonstrated that desmin-positive cells in the glomeruli of these chimeric mice were also positive for GFP. Among glomerular cells isolated from [GFP-->B6] mice 24 wk after bone marrow transplantation and then cultured, the majority of cells (approximately 84%) stained for desmin and approximately 60% of the desmin-positive cells expressed GFP. In addition, these GFP-positive cells in the cultures contracted in response to angiotensin II stimulation. These results suggest that bone marrow-derived cells may have the potential to differentiate into glomerular mesangial cells.
authors: Department of Internal Medicine, Jikei University School of Medicine, Tokyo, Japan.|Department of Microbiology, Jikei University School of Medicine, Tokyo, Japan.|Department of Molecular Pathology, Tokyo Metropolitan Institute of Gerontology, Tokyo, Japan.
authors: Toshiyuki
authors: T
authors: Imasawa
authors: Department of Internal Medicine, Jikei University School of Medicine, Tokyo, Japan.
authors: Yasunori
authors: Y
authors: Utsunomiya
authors: Department of Internal Medicine, Jikei University School of Medicine, Tokyo, Japan.
authors: Tetsuya
authors: T
authors: Kawamura
authors: Department of Microbiology, Jikei University School of Medicine, Tokyo, Japan.
authors: Y U
authors: YU
authors: Zhong
authors: Department of Internal Medicine, Saitama Medical Center, Saitama Medical School, Saitama, Japan.|Department of Molecular Pathology, Tokyo Metropolitan Institute of Gerontology, Tokyo, Japan.
authors: Ryuji
authors: R
authors: Nagasawa
authors: Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.
authors: Masaru
authors: M
authors: Okabe
authors: Department of Molecular Pathology, Tokyo Metropolitan Institute of Gerontology, Tokyo, Japan.
authors: Naoki
authors: N
authors: Maruyama
authors: Department of Internal Medicine, Jikei University School of Medicine, Tokyo, Japan.
authors: Tatsuo
authors: T
authors: Hosoya
authors: Department of Microbiology, Jikei University School of Medicine, Tokyo, Japan.
authors: Tsuneya
authors: T
authors: Ohno
mesh_terms: D000804:Angiotensin II / Q000494:pharmacology; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D016026:Bone Marrow Transplantation; D002454:Cell Differentiation; D002478:Cells, Cultured; D003893:Desmin / Q000378:metabolism; D005260:Female; D005920:Glomerular Mesangium / Q000166:cytology* / Q000378:metabolism; D049452:Green Fluorescent Proteins; D007202:Indicators and Reagents / Q000378:metabolism; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic / Q000235:genetics; D013997:Time Factors; D014019:Tissue Donors
chemical_list: D003893:Desmin; D007202:Indicators and Reagents; D008164:Luminescent Proteins; D000804:Angiotensin II; D049452:Green Fluorescent Proteins