title: Analysis of canine parvovirus sequences from wolves and dogs isolated in Italy.
abstract: The VP2 genes of Italian canine parvovirus (CPV) type 2 strains isolated from dogs and wolves were sequenced and a three-dimensional model of the VP2 capsid protein was constructed. Two mutations were detected in the VP2 sequences of the Italian strains: one at residue 297 and one at residue 265. Variant 297 is the predominant CPV isolate in Europe, whereas variant 265 has never been detected before. The mutation at residue 265 causes a disruption in a G strand of the beta-barrel in the VP2 protein. Data on strains isolated from wolves demonstrated that the same strain of CPV can circulate among domestic and wild canids; therefore, this result leads us to exclude the possibility that a separate parvovirus pool exists in wild populations.
authors: Dipartimento di Sanità Pubblica Veterinaria e Patologia Animale, Università degli Studi di Bologna, Via Tolara di Sopra 50, 40064-Ozzano Emilia (BO), Italy1.
authors: Mara
authors: M
authors: Battilani
authors: Dipartimento di Sanità Pubblica Veterinaria e Patologia Animale, Università degli Studi di Bologna, Via Tolara di Sopra 50, 40064-Ozzano Emilia (BO), Italy1.
authors: Alessandra
authors: A
authors: Scagliarini
authors: Istituto Zooprofilattico Sperimentale delle Venezie, Via Romea 14/A, 35020-Legnaro (Padova), Italy2.
authors: Ernesto
authors: E
authors: Tisato
authors: Istituto Zooprofilattico Sperimentale delle Venezie, Via Romea 14/A, 35020-Legnaro (Padova), Italy2.
authors: Carlo
authors: C
authors: Turilli
authors: Unità di Biocomputing Centro Interdipartimentale per le Ricerche Biotecnologiche (CIRB)3 and Dipartimento di Biologia4, Università degli Studi di Bologna, Via Irnerio 42, 40126-Bologna, Italy.
authors: Irene
authors: I
authors: Jacoboni
authors: Unità di Biocomputing Centro Interdipartimentale per le Ricerche Biotecnologiche (CIRB)3 and Dipartimento di Biologia4, Università degli Studi di Bologna, Via Irnerio 42, 40126-Bologna, Italy.
authors: Rita
authors: R
authors: Casadio
authors: Dipartimento di Sanità Pubblica Veterinaria e Patologia Animale, Università degli Studi di Bologna, Via Tolara di Sopra 50, 40064-Ozzano Emilia (BO), Italy1.
authors: Santino
authors: S
authors: Prosperi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002213:Capsid / Q000737:chemistry / Q000235:genetics; D004283:Dog Diseases / Q000821:virology*; D004285:Dogs; D005243:Feces / Q000821:virology; D005759:Gastroenteritis / Q000662:veterinary*; D005814:Genes, Viral; D007558:Italy; D008969:Molecular Sequence Data; D009154:Mutation; D010322:Parvoviridae Infections / Q000662:veterinary*; D017993:Parvovirus, Canine / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment; D019596:Wolves / Q000821:virology*