In vivo and in vitro techniques to determine the biological activity of food allergens.
Methods for determination of the biological activity of food allergens comprise both determination of the allergenic potency, i.e. the capability to elicit an allergic reaction in an already sensitized individual, and the allergenic potential, i.e. the risk for sensitizing a hitherto non-allergic individual. Several methods are discussed for determination of potency including the double-blinded placebo-controlled food challenge, skin testing, in vitro effector cell assays such as basophil histamine release, and IgE-based techniques such as RAST and RAST inhibition. No reliable methods have yet been developed which can predict the allergenic potential of a food or a food allergen. The progress in the areas of stability studies and animal models for food allergy are discussed.
Poulsen
L K
LK
Allergy Unit, National University Hospital, København N, Denmark. lkpallgy@inet.uni2.dk
D000485:Allergens / Q000378:metabolism / Q000502:physiology*; D000818:Animals; D004311:Double-Blind Method; D005512:Food Hypersensitivity / Q000276:immunology*; D006801:Humans; D007073:Immunoglobulin E / Q000378:metabolism; D010919:Placebos; D012882:Skin Tests
D000485:Allergens; D010919:Placebos; D007073:Immunoglobulin E