[Genetic diversity of a natural population of Sinorhizobium meliloti, detected during analysis of a cryptic plasmid and ISRm2011-2 fingerprints].
Fifty-six natural strains of alfalfa nodule bacteria were isolated from samples of the soil under wild legume and alfalfa in two different field sites of Irkutsk oblast. Based on the results of analysis of plasmid profile, 11 different types of strains were detected, and 43 types were identified based on the results of hybridization with the insertion sequence element ISRm2011-2. Significant differences were found in the plasmid profile and IS fingerprints between strains isolated from the soil under alfalfa and the soil under legume. In contrast, strains growing at some distance from each other differed only in the IS fingerprints. From a comparison of results obtained in the assessment of plasmid profile and in analysis of IS fingerprints with results of RFLP analysis in strains, the conclusion about the transference of cryptic plasmids between strains and genetic rearrangements in strains of this population was drawn.
Andronov
E E
EE
All-Russia Research Institute of Agricultural Microbiology, Russian Academy of Agricultural Sciences, St. Petersburg, Pushkin, 196608 Russia.
Rumiantseva
M L
ML
Simarov
B V
BV
D016172:DNA Fingerprinting; D014644:Genetic Variation; D000455:Medicago sativa / Q000382:microbiology; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D012150:Polymorphism, Restriction Fragment Length; D016962:Sinorhizobium meliloti / Q000235:genetics*