Effect of novel platinum complexes on survival rate of B16 and Cl S91 melanoma cells and oxidation stress in vitro.
BACKGROUND
The aim of study was the assessment of two novel platinum complexes and their cytotoxic effect on murine melanoma cells. The evaluation of possible oxidation stress in neoplastic cells coexistent with the effect of these cytostatic agents.


METHODS
Two lines of murine metastatic melanomas: B16 and Cl S91 were selected for the experiments. Two platinum compounds were tested: cis-dichloro(dimethylsulphoxide)(1-beta-D-ribofuranosyl-1,2,4-triazol-3-carboxyamide)platinum (II) called Pt-complex 1 and chloro(dimethylsulphoxide)(1-beta-D-ribofuranosyl-1,2,4-triazol-3-carboxyamide)platinum (II) called Pt-complex 2. Melanoma cells were incubated for 24 hours with the platinum complexes mentioned above and culture medium at the concentration of 0.01, 0.05 and 0.5 mg/ml. Survival rate of melanoma cells, superoxide dismutase (SOD) activity, the concentration of thiobarbituric acid reactive substances (TBARS) and the content of conjugated dienes were estimated.


RESULTS
A number of statistically significant relationships between analysed parameters were observed. Negative correlation ratios were found between increasing levels of analysed platinum complexes and the survival rate of the cells of both melanoma lines as well as between the survival rate of Cl S91 melanoma cells and the level of lipid peroxidation products and SOD activity. On the other hand, there was a positive correlation between increasing platinum complexes and the level of lipid peroxidation products as well as between the survival rate of B16 melanoma cells and SOD activity and the content of CD.


CONCLUSIONS
Oxygen free radicals may play an important role in the development of melanoma and cytostatic agents disturb the balance between generation and removal of reactive oxygen forms in melanoma cells.
Drewa
T
T
Department of Biology, Medical University in Bydgoszcz, Poland.
Wozniak
A
A
Drewa
G
G
Olszewska
D
D
Wozniak
B
B
Wysocki
M
M
Szlyk
E
E
Lakomska
A
A
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects*; D008546:Melanoma, Experimental / Q000201:enzymology / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009944:Organoplatinum Compounds / Q000494:pharmacology*; D018384:Oxidative Stress; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D000970:Antineoplastic Agents; D009944:Organoplatinum Compounds; D017392:Thiobarbituric Acid Reactive Substances; D013482:Superoxide Dismutase