title: Natural paratuberculosis infection in rabbits in Scotland.
abstract: Natural paratuberculosis infection of rabbits (Oryctolagus cuniculus) was recently diagnosed in Scotland, and an investigation into the pathology of the disease in wild rabbits is reported in this paper. Evidence of Mycobacterium avium subsp. paratuberculosis (M.a. paratuberculosis) infection was detected in 22% of 110 rabbits; the organism was cultured from 17 of 110 rabbits, and histopathological lesions consistent with M.a. paratuberculosis infection were noted in 18 of 98 rabbits examined. No macroscopical lesions suggestive of M.a. paratuberculosis infection were observed. The histopathological lesions were either severe or mild. Severe lesions consisted of extensive macrophage granulomata and numerous giant cells, with many intracellular acid-fast bacteria in the small intestine. For the examination of formalin-fixed, paraffin wax-embedded tissues, neither immunohistochemistry nor the polymerase chain reaction was as sensitive a method of diagnosis as histopathology.
authors: Moredun Research Institute, International Research Centre, Pentlands Science Park, Bush Loan, Penicuik, Midlothian, EH26 OPZ, Scotland.
authors: P M
authors: PM
authors: Beard
authors: S M
authors: SM
authors: Rhind
authors: D
authors: D
authors: Buxton
authors: M J
authors: MJ
authors: Daniels
authors: D
authors: D
authors: Henderson
authors: A
authors: A
authors: Pirie
authors: K
authors: K
authors: Rudge
authors: A
authors: A
authors: Greig
authors: M R
authors: MR
authors: Hutchings
authors: K
authors: K
authors: Stevenson
authors: J M
authors: JM
authors: Sharp
mesh_terms: D000818:Animals; D000835:Animals, Wild; D000942:Antigens, Bacterial / Q000032:analysis; D002417:Cattle; D004269:DNA, Bacterial / Q000032:analysis; D019084:Fluorescent Antibody Technique, Indirect / Q000662:veterinary; D006099:Granuloma / Q000382:microbiology / Q000473:pathology; D007421:Intestine, Small / Q000382:microbiology / Q000473:pathology; D008198:Lymph Nodes / Q000382:microbiology / Q000473:pathology; D008643:Mesentery / Q000382:microbiology / Q000473:pathology; D016927:Mycobacterium avium subsp. paratuberculosis / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D010283:Paratuberculosis / Q000453:epidemiology / Q000382:microbiology / Q000473:pathology*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011817:Rabbits; D012606:Scotland / Q000453:epidemiology
chemical_list: D000942:Antigens, Bacterial; D004269:DNA, Bacterial