Basic fibroblast growth factor enhances in vitro mineralization of rat bone marrow stromal cells grown on non-woven hyaluronic acid based polymer scaffold.
A biodegradable non-woven hyaluronic acid polymer scaffold (Hyaff 11) was analysed in vitro as a carrier vehicle for differentiation and mineralization of rat bone marrow stromal cells (BMSC). BMSC were grown on Hyaff 11 in a mineralizing medium in the presence/absence of basic fibroblast growth factor (bFGF). Osteoblastic differentiation was investigated by light and electron microscopy analysing the expression of osteogenic markers: calcium, alkaline phosphatase (AP), osteopontin (OP), bone sialoprotein (BSP) and collagen type 1. We also measured proliferation, AP activity and mRNA expression of AP and osteocalcin (OC). Electron microscopy and Toluidine-blue staining demonstrated that bFGF accelerated (day 20 vs. day 40) and increased mineralization. With bFGF, calcium, OP and BSP were strongly enhanced at day 40, whereas AP decreased. Our in vitro results demonstrate that Hyaff 11 is a useful vehicle for growth, differentiation and mineralization of rat BMSC, and that it permits bone development.
Lisignoli
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G
Laboratorio di Immunologia e Genetica. Istituti Ortopedici Rizzoli, Bologna, Italy.
Zini
N
N
Remiddi
G
G
Piacentini
A
A
Puggioli
A
A
Trimarchi
C
C
Fini
M
M
Maraldi
N M
NM
Facchini
A
A
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D001854:Bone Marrow Cells / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods; D002455:Cell Division; D002478:Cells, Cultured; D024042:Collagen Type I / Q000378:metabolism; D004396:Coloring Agents / Q000494:pharmacology; D003470:Culture Media; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D006820:Hyaluronic Acid / Q000737:chemistry*; D058574:Integrin-Binding Sialoprotein; D007700:Kinetics; D008854:Microscopy, Electron; D010006:Osteoblasts / Q000166:cytology; D015675:Osteocalcin / Q000378:metabolism; D053495:Osteopontin; D011108:Polymers / Q000737:chemistry*; D011485:Protein Binding; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011916:Rats, Inbred F344; D012795:Sialoglycoproteins / Q000378:metabolism; D017154:Stromal Cells / Q000166:cytology*; D013997:Time Factors; D014048:Tolonium Chloride / Q000494:pharmacology
D001672:Biocompatible Materials; D024042:Collagen Type I; D004396:Coloring Agents; D003470:Culture Media; C546873:Ibsp protein, rat; D058574:Integrin-Binding Sialoprotein; D011108:Polymers; D012333:RNA, Messenger; D012795:Sialoglycoproteins; C506897:Spp1 protein, rat; D016222:Fibroblast Growth Factor 2; D015675:Osteocalcin; D053495:Osteopontin; D014048:Tolonium Chloride; D006820:Hyaluronic Acid; D000469:Alkaline Phosphatase; D002118:Calcium