title: The double mechanism of incompatibility between lambda plasmids and Escherichia coli dnaA(ts) host cells.
abstract: For plasmids derived from bacteriophage lambda, the initiation of bidirectional DNA replication from orilambda depends on the stimulation of transcription from the p(R) promoter by the host replication initiator protein DnaA. Certain Escherichia coli dnaA(ts) mutants cannot be transformed by wild-type lambda plasmids even at the temperature permissive to cell growth. This plasmid-host incompatibility appeared to be due to inefficient stimulation of transcription from the p(R) promoter by the mutant DnaA protein. This paper shows that there is a second mechanism for the incompatibility between lambda plasmids and dnaA(ts) hosts, exemplified in this study by the dnaA46 mutant. This is based on the competition between the lambda P protein and the host DnaA and DnaC proteins for DnaB helicase. Both mechanisms must be operative for the incompatibility.
authors: Department of Molecular Biology, University of Gdańsk, Kladki 24, 80-822 Gdańsk, Poland1.
authors: Monika
authors: M
authors: Glinkowska
authors: Department of Molecular Biology, University of Gdańsk, Kladki 24, 80-822 Gdańsk, Poland1.
authors: Grażyna
authors: G
authors: Konopa
authors: Laboratory of Molecular Biology (affiliated with the University of Gdańsk), Polish Academy of Sciences, Kładki 24, 80-822 Gdańsk, Poland2.
authors: Alicja
authors: A
authors: Węgrzyn
authors: Department of Molecular Biology, University of Gdańsk, Kladki 24, 80-822 Gdańsk, Poland1.
authors: Anna
authors: A
authors: Herman-Antosiewicz
authors: Max-Planck-Institut für Molekulare Genetik, Ihnestrasse 73, D-14195 Berlin-Dahlem, Germany3.
authors: Christoph
authors: C
authors: Weigel
authors: Max-Planck-Institut für Molekulare Genetik, Ihnestrasse 73, D-14195 Berlin-Dahlem, Germany3.
authors: Harald
authors: H
authors: Seitz
authors: Max-Planck-Institut für Molekulare Genetik, Ihnestrasse 73, D-14195 Berlin-Dahlem, Germany3.
authors: Walter
authors: W
authors: Messer
authors: Marine Biology Centre, Polish Academy of Sciences, Św. Wojciecha 5, 81-347 Gdynia, Poland4.|Department of Molecular Biology, University of Gdańsk, Kladki 24, 80-822 Gdańsk, Poland1.
authors: Grzegorz
authors: G
authors: Węgrzyn
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D010582:Bacteriophage lambda / Q000235:genetics*; D004265:DNA Helicases / Q000378:metabolism; D004261:DNA Replication; D004268:DNA-Binding Proteins / Q000235:genetics*; D053488:DnaB Helicases; D004926:Escherichia coli / Q000821:virology*; D009154:Mutation; D010957:Plasmids / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics; D018741:Replication Origin / Q000235:genetics; D015533:Transcriptional Activation
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C066312:DnaA protein, Bacteria; D004265:DNA Helicases; D053488:DnaB Helicases