Pneumocystis carinii carriage among cystic fibrosis patients, as detected by nested PCR.
A total of 137 sputa from 95 consecutive cystic fibrosis (CF) patients undergoing routine bacteriological surveillance were analyzed for Pneumocystis carinii colonization using nested PCR. Seven of 95 patients (7.4%) were PCR positive, suggesting that P. carinii carriage may exist among CF patients due to their underlying pulmonary disease.
Sing
A
A
Max von Pettenkofer-Institut für Hygiene und Medizinische Mikrobiologie, 80336 Munich, Germany. sing@m3401.mpk.md.uni-muenchen.de
Geiger
A M
AM
Hogardt
M
M
Heesemann
J
J
D000328:Adult; D002353:Carrier State / Q000382:microbiology*; D003550:Cystic Fibrosis / Q000382:microbiology*; D004271:DNA, Fungal / Q000032:analysis; D005260:Female; D006801:Humans; D008297:Male; D011010:Pneumocystis / Q000235:genetics / Q000302:isolation & purification*; D016720:Pneumocystis Infections / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D013183:Sputum / Q000382:microbiology
D004271:DNA, Fungal