Correlation between EGF receptor expression and peplomycin cytotoxicity in squamous cell carcinoma cell lines.
The relationship between sensitivity to anti-cancer agents and EGF receptor expression on squamous cell carcinoma (SCC) cells was investigated. The cytotoxicity of peplomycin (PEP) was correlated with the number of the EGF receptors expressed on the cancer cells, but no correlations were found between the cytotoxicity of adriamycin and cisplatin and EGF receptors. Addition of TNFalpha increased the number of EGF receptors in the SCC cell lines 1.5- to 2-fold. The cytotoxic effect of combined administration of PEP and TNFalpha was correlated with the number of EGF receptors, and produced a 2- to 5-fold increase in IC50 compared with administration of PEP alone. These observations suggest that EGF receptor expression is closely associated with the cytotoxic effect of PEP on SCC cells.
Osaku
M
M
Department of Surgery, Kitasato Institute Hospital, Tokyo 108-8642, Japan.
Ueda
M
M
Miyakawa
T
T
Toyoda
H
H
Uesato
K
K
Yamada
Y
Y
Asanuma
F
F
Ando
N
N
Ozawa
S
S
Kitagawa
Y
Y
Kitajima
M
M
D000903:Antibiotics, Antineoplastic / Q000633:toxicity*; D000911:Antibodies, Monoclonal / Q000627:therapeutic use; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy* / Q000378:metabolism; D002470:Cell Survival; D002945:Cisplatin / Q000633:toxicity; D004317:Doxorubicin / Q000633:toxicity; D004354:Drug Screening Assays, Antitumor; D066246:ErbB Receptors / Q000378:metabolism*; D004938:Esophageal Neoplasms / Q000188:drug therapy* / Q000378:metabolism; D005260:Female; D005887:Gingival Neoplasms / Q000188:drug therapy* / Q000378:metabolism; D006801:Humans; D017663:Peplomycin / Q000633:toxicity*; D012044:Regression Analysis; D014407:Tumor Cells, Cultured / Q000187:drug effects* / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D014846:Vulvar Neoplasms / Q000188:drug therapy* / Q000378:metabolism
D000903:Antibiotics, Antineoplastic; D000911:Antibodies, Monoclonal; D014409:Tumor Necrosis Factor-alpha; D017663:Peplomycin; D004317:Doxorubicin; D066246:ErbB Receptors; D002945:Cisplatin