Helicobacter mustelae lipid A structure differs from that of Helicobacter pylori.
The lipid A structure of the Gram-negative bacterium Helicobacter mustelae, a ferret gastric pathogen responsible for the onset of gastric diseases in its host, was investigated. Two variant lipid A structures were found in the same strain. One structure contained a bisphosphorylated beta-(1-->6)-linked D-glucosamine backbone disaccharide with hydroxytetradecanoic acid in amide linkages. Unlike the structure described for the lipid A of the related human Helicobacter pylori gastric pathogen, which contains a C1 phosphate moiety, this lipid A presented phosphate groups at both the C1 and C4' positions, and contained no octadecanoyl fatty acid, which is present in H. pylori. The second lipid A structure had a different fatty acid composition in that 3-OH C(16) replaced most of the amide-linked 3-OH C(14).
Thérisod
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Equipe Endotoxines, UMR 8619 du Centre National de la Recherche Scientifique, Biochimie, Université de Paris-Sud, Orsay, France.
Monteiro
M A
MA
Perry
M B
MB
Caroff
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M
D000818:Animals; D002236:Carbohydrate Conformation; D005227:Fatty Acids / Q000032:analysis / Q000737:chemistry; D005289:Ferrets / Q000382:microbiology; D005944:Glucosamine / Q000378:metabolism; D016998:Helicobacter / Q000737:chemistry*; D016480:Helicobacter pylori / Q000737:chemistry*; D008050:Lipid A / Q000737:chemistry*; D008070:Lipopolysaccharides / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D009226:Myristates / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D005227:Fatty Acids; D008050:Lipid A; D008070:Lipopolysaccharides; D009226:Myristates; D005944:Glucosamine