Green tea polyphenol extract attenuates inflammation in interleukin-2-deficient mice, a model of autoimmunity.
Green tea polyphenols (GrTP) have been previously shown to decrease endotoxin-induced tumor necrosis factor-alpha production and lethality in mice. Our present studies demonstrate that GrTP inhibit inflammatory responses and may be useful in treating chronic inflammatory states, such as inflammatory bowel disease. In this preliminary study, we examined whether GrTP decrease disease activity in interleukin-2-deficient (IL-2(-/-) mice. Eight-week old IL-2(-/-) C57BL/6J mice who were fed nonpurified diet were randomly assigned to receive water with GrTP (5 g/L) or water alone (control) for up to 6 wk. After 1 wk, explant colon and lamina propria lymphocyte (LPL) cultures were established from a subgroup of mice and supernatants collected. Culture supernatants from GrTP-treated mice showed decreased spontaneous interferon-gamma and tumor necrosis factor-alpha secretion compared with that of controls. At 6 wk, the GrTP group had less severe colitis as demonstrated by lower histologic scores and wet colon weights. This was associated with lower plasma levels of serum amyloid A, increased weight gain and improved hematocrits. These results show that GrTP attenuated inflammation in IL-2(-/-) mice and suggest a role for GrTP in treating chronic inflammatory diseases such as inflammatory bowel disease.
Varilek
G W
GW
Graduate Program in Nutritional Sciences, University of Kentucky, Lexington, KY 40536, USA.
Yang
F
F
Lee
E Y
EY
deVilliers
W J
WJ
Zhong
J
J
Oz
H S
HS
Westberry
K F
KF
McClain
C J
CJ
D000682:Amyloid / Q000097:blood; D000744:Anemia, Hemolytic, Autoimmune / Q000097:blood / Q000188:drug therapy; D000818:Animals; D001327:Autoimmune Diseases / Q000188:drug therapy* / Q000276:immunology; D002478:Cells, Cultured; D003106:Colon / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D046508:Culture Techniques; D004195:Disease Models, Animal; D005260:Female; D005419:Flavonoids; D006400:Hematocrit; D015212:Inflammatory Bowel Diseases / Q000188:drug therapy* / Q000276:immunology / Q000517:prevention & control; D007371:Interferon-gamma / Q000378:metabolism; D007376:Interleukin-2 / Q000172:deficiency* / Q000276:immunology; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010636:Phenols / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D008517:Phytotherapy; D011108:Polymers / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D059808:Polyphenols; D011897:Random Allocation; D013662:Tea / Q000737:chemistry* / Q000627:therapeutic use; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D015430:Weight Gain
D000682:Amyloid; D005419:Flavonoids; D007376:Interleukin-2; D010636:Phenols; D011108:Polymers; D059808:Polyphenols; D013662:Tea; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma