Grid-immunoblotting: a fast and simple technique to test multiple allergens with small amounts of antibody for cross-reactivity.
Grid-immunoblotting is a procedure that allows the simultaneous testing of up to 20 different antibodies such as monoclonal antibody-containing hybridoma supernatants or human sera for specific antibodies to up to 20 different antigens or allergens on a single sheet of nitrocellulose membrane. Since only 150 to 200 microl of antibody-containing solution are required this technique is uniquely suited to test growing hybridomas and small amounts of sera (e.g. mouse and children's sera). Compared to a standard ELISA, approximately ten times less antibody is needed to obtain the same information.
Reese
G
G
Tulane University Medical Center, New Orleans, LA 70112, USA. greese@tulane.edu
Schmechel
D
D
Ayuso
R
R
Lehrer
S B
SB
D000485:Allergens / Q000276:immunology*; D000818:Animals; D000906:Antibodies / Q000276:immunology*; D001230:Aspergillus / Q000276:immunology; D015153:Blotting, Western / Q000379:methods*; D003429:Cross Reactions; D051379:Mice; D010407:Penicillium / Q000276:immunology
D000485:Allergens; D000906:Antibodies