Genomic homogeneity in fibrolamellar carcinomas.
BACKGROUND
Fibrolamellar carcinoma (FLC) is a variant of hepatocellular carcinoma (HCC) with distinctive clinical and histological features. To date there have been few studies on the genotypic aspects of FLC and no previous attempts have been made to use the arbitrarily primed-polymerase chain reaction (AP-PCR) technique to detect genetic alterations in this disease.


OBJECTIVE
The aim of this study was to assess the degree of genomic heterogeneity of FLC using the AP-PCR technique.


METHODS
A total of 50 tissue samples of primary and metastatic FLCs from seven patients were microdissected. AP-PCR amplification of each genomic DNA sample was carried out using two arbitrary primers.


RESULTS
DNA fingerprints of the primary FLCs and all their metastatic lesions (both synchronous and metachronous disease) were identical in an individual patient. The fingerprints were different between tumours of different patients. No evidence of intratumour heterogeneity was observed.


CONCLUSIONS
Such genomic homogeneity in FLCs may explain their indolent growth. The absence of clonal evolution, which is present in other tumours (particularly HCCs), may explain the distinct behaviour in this tumour. The tumorigenic pathway and degree of somatic genomic changes in this disease may be less complex than in HCC.
Sirivatanauksorn
Y
Y
University Department of Surgery, Royal Free and University College Medical School, Royal Free Hospital, Pond Street, London NW3 2QG, UK.
Sirivatanauksorn
V
V
Lemoine
N R
NR
Williamson
R C
RC
Davidson
B R
BR
D006528:Carcinoma, Hepatocellular / Q000235:genetics*; D016172:DNA Fingerprinting; D019143:Evolution, Molecular; D005260:Female; D018740:Genetic Heterogeneity; D015894:Genome, Human; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics*; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods