Semiquantitative analysis of urinary low protein levels using silver dot blot assay.
We designed a semiquantitative analysis of urinary low protein levels using silver dot blot assay. In this method, 3 microl of urine are blotted to one dot onto a cellulose acetate membrane, which is stained by a colloidal silver staining reagent, and the optical density of the silver stained urinary protein is measured at 500 nm using a densitometer. There was a good linearity between 2.5 mg/L and 100 mg/L and a gentle linearity between 100 mg/L and 200 mg/L, and the minimum sensitivity was 2.5 mg/L. This method is suitable for semiquantitative analysis of urinary protein levels less than 300 mg/L, which can not be determined precisely by dipstick.
Matsuda
K
K
School of Allied Health Sciences, Faculty of Medicine, Tokyo Medical and Dental University, Tokyo, Japan.
Hiratsuka
N
N
Kurihara
Y
Y
Shiba
K
K
D000419:Albuminuria / Q000652:urine; D000814:Aniline Compounds; D001536:Bence Jones Protein / Q000652:urine; D002482:Cellulose / Q000031:analogs & derivatives; D003102:Colloids; D006801:Humans; D007202:Indicators and Reagents; D009088:Mucoproteins / Q000652:urine; D011507:Proteinuria / Q000652:urine*; D012680:Sensitivity and Specificity; D016622:Silver Staining; D058949:Uromodulin; D005719:gamma-Globulins / Q000652:urine
D000814:Aniline Compounds; D003102:Colloids; D007202:Indicators and Reagents; D009088:Mucoproteins; C548612:UMOD protein, human; D058949:Uromodulin; D005719:gamma-Globulins; C005062:acetylcellulose; C002712:nigrosin; D002482:Cellulose; D001536:Bence Jones Protein