Identification of N-terminal autodigestion target site in subtilisin ALP I.
Autodigestion of subtilisin ALP I (ALP I), secreted from the alkalophilic Bacillus sp. NKS-21 and its predicted amino acid sequence having about 60% identity with other alkaline subtilisins, was examined under alkaline conditions. At the alkaline pH of 12, ALP I was rapidly degraded, and almost no breakdown products were detectable. However, by incubating ALP I at 5 degrees C for an extended time, a couple of specific peptides (26.7 kDa and 25.6 kDa) were accumulated. Each of them was purified and amino acid sequences of these fragments were found. Both peptides appeared to start at Gly-19 of the mature sequence of ALP I.
Maeda
H
H
Division of Life Science, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan. maeda@biochem.tohoku.ac.jp
Yamagata
Y
Y
Ichishima
E
E
Nakajima
T
T
D000595:Amino Acid Sequence; D001407:Bacillus / Q000201:enzymology; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation; D013381:Subtilisins / Q000737:chemistry / Q000378:metabolism*
D013381:Subtilisins; C435719:subtilisin ALP I