Molecular detection of human calicivirus in young children hospitalized with acute gastroenteritis in Melbourne, Australia, during 1999.
Reverse transcription-PCR and sequence analysis identified calciviruses in 32 of 60 stool specimens (negative for other enteric pathogens) obtained from children admitted to our hospital with acute gastroenteritis. The overall annual incidence rate for calcivirus was 9% (32 of 354 children). Molecular analysis identified 30 "Norwalk-like virus" genogroup II (predominantly Lordsdale cluster) and 2 "Sapporo-like virus" strains.
Kirkwood
C D
CD
Department of Gastroenterology and Clinical Nutrition, Murdoch Childrens Research Institute, Royal Children's Hospital, Victoria, Australia. Kirkwooc@cryptic.rch.unimelb.edu.au
Bishop
R F
RF
D000208:Acute Disease; D001315:Australia / Q000453:epidemiology; D002139:Caliciviridae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D017250:Caliciviridae Infections / Q000453:epidemiology* / Q000821:virology; D002675:Child, Preschool; D005759:Gastroenteritis / Q000453:epidemiology* / Q000821:virology; D006760:Hospitalization; D006801:Humans; D007223:Infant; D009663:Norwalk virus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA