Assessment of sediment contamination by spermiotoxicity and embryotoxicity bioassays with sea urchins (Paracentrotus lividus) and oysters (Crassostrea gigas).
Gametes (sperm) and fertilized eggs (embryos) of the Mediterranean sea urchin, Paracentrotus lividus, and the Japanese oyster, Crassostrea gigas, were used to investigate the toxicity of two marine sediments, one polluted by polycyclic aromatic hydrocarbons (PAH) and the other by heavy metals. The sediment samples were freeze-dried for storage, and three different treatments were used for analysis: whole sediment, unfiltered elutriate, and filtered elutriate. The two sediments were toxic to sea urchin spermatozoa but not to oyster spermatozoa, and embryotoxicity was almost always the more sensitive endpoint for toxicity assessment. As a rule, whole sediment was more toxic than the elutriates by nearly two orders of magnitude. With respect to embryotoxicity, the whole sediments and the elutriates of the PAH-contaminated sediment were more toxic to oyster embryos, whereas the elutriates of the sediment polluted by heavy metals had stronger effects on sea urchin embryos. The results confirm that bioassays with Japanese oyster embryos provide a more sensitive appraisal of toxicity in the marine environment than bioassays with other developmental stages. As a whole, Mediterranean sea urchins and Japanese oysters were similar in overall sensitivity and are therefore both equally suited as bioassay organisms, but tests with oysters are more reproducible because of the better performance of the controls.
Geffard
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IFREMER, DEL/PC, Station d'Arcachon, Quai Silhouette, 33120 Arcachon, France. ogeffard@ifremer.fr
Budzinski
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H
Augagneur
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S
Seaman
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MN
His
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E
D000818:Animals; D001681:Biological Assay; D004625:Embryo, Nonmammalian / Q000187:drug effects*; D023381:Endpoint Determination; D004781:Environmental Exposure; D004785:Environmental Pollutants / Q000009:adverse effects*; D019015:Geologic Sediments / Q000737:chemistry*; D008297:Male; D019216:Metals, Heavy / Q000009:adverse effects*; D010125:Ostreidae / Q000254:growth & development / Q000502:physiology*; D011084:Polycyclic Aromatic Hydrocarbons / Q000009:adverse effects*; D015203:Reproducibility of Results; D012617:Sea Urchins / Q000254:growth & development / Q000502:physiology*; D012680:Sensitivity and Specificity; D013094:Spermatozoa / Q000187:drug effects* / Q000502:physiology; D018675:Toxicity Tests
D004785:Environmental Pollutants; D019216:Metals, Heavy; D011084:Polycyclic Aromatic Hydrocarbons