Experimental osteoinduction by recombinant human bone morphogeneticprotein 2 in tissue with low blood flow: a study in rats.
We have compared osteoinduction in soft tissues by 0, 2, 10, and 50 microg of recombinant human bone morphogenetic protein-2 (rhBMP-2) in Wistar rats with a surgically compromised blood supply and controls with a normal blood supply (n = 5 in each group). Three weeks later in the radiographic evaluation, the volume of bone formation in the low-blood-flow groups was similar to that in the controls, and was dependent on the concentration of implanted rhBMP-2. On histological evaluation much woven bone was noted in the low-blood-flow groups, but the volume of bone was less than in the controls. Alkaline phosphatase activity was less and calcium content in the low-blood-flow groups were also less than in the control rats. However, their values increased dose-dependently with implanted rhBMP-2. These results suggest that the osteoinducting activity of rhBMP-2 is reduced in tissues with a low blood supply, but higher doses of rhBMP-2 can be used in compromised tissue.
Fujimura
K
K
Department of Oral and Maxillofacial Surgery, Kanazawa Medical University, Ishikawa Prefecture, Japan.
Bessho
K
K
Kusumoto
K
K
Konishi
Y
Y
Ogawa
Y
Y
Iizuka
T
T
D000469:Alkaline Phosphatase / Q000096:biosynthesis; D000818:Animals; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000494:pharmacology*; D005263:Femoral Artery; D005269:Femur / Q000098:blood supply / Q000000981:diagnostic imaging; D006801:Humans; D008026:Ligation; D008297:Male; D018482:Muscle, Skeletal / Q000098:blood supply; D010012:Osteogenesis / Q000187:drug effects*; D011859:Radiography; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000494:pharmacology; D012039:Regional Blood Flow; D016212:Transforming Growth Factor beta
C525718:BMP2 protein, human; C525720:Bmp2 protein, rat; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; C499469:recombinant human bone morphogenetic protein-2; D000469:Alkaline Phosphatase