New plasmid system to select for Saccharomyces cerevisiae purine-cytosine permease affinity mutants.
The FCY2 gene of Saccharomyces cerevisiae encodes a purine-cytosine permease (PCP) that mediates the active transport of purines and cytosine. A structure-function model for this PCP has been recently proposed. In this study, we developed a plasmid-based system that generated a number of affinity-mutated alleles, enabling us to define new amino acids critical for permease function.
Wagner
R
R
Laboratoire de Microbiologie et de Génétique, ESA 7010 Université Louis Pasteur/CNRS, Institut de Botanique, F-67083 Strasbourg Cedex, France.
Straub
M L
ML
Souciet
J L
JL
Potier
S
S
de Montigny
J
J
D000483:Alleles; D002352:Carrier Proteins / Q000235:genetics*; D004271:DNA, Fungal; D026901:Membrane Transport Proteins / Q000235:genetics*; D009154:Mutation; D020125:Mutation, Missense; D033702:Nucleobase Transport Proteins; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins
D002352:Carrier Proteins; D004271:DNA, Fungal; C043181:FCY2 protein, S cerevisiae; D026901:Membrane Transport Proteins; D033702:Nucleobase Transport Proteins; D029701:Saccharomyces cerevisiae Proteins