title: Sec-dependent membrane protein insertion: sequential interaction of nascent FtsQ with SecY and YidC.
abstract: Recent studies identified YidC as a novel membrane factor that may play a key role in membrane insertion of inner membrane proteins (IMPs), both in conjunction with the Sec-translocase and as a separate entity. Here, we show that the type II IMP FtsQ requires both the translocase and, to a lesser extent, YidC in vivo. Using photo-crosslinking we demonstrate that the transmembrane (TM) domain of the nascent IMP FtsQ inserts into the membrane close to SecY and lipids, and moves to a combined YidC/lipid environment upon elongation. These data are consistent with a crucial role for YidC in the lateral transfer of TM domains from the Sec translocase into the lipid bilayer.
authors: Department of Microbiology, Institute of Molecular Biological Sciences, De Boelelaan 1087, 1081 HV Amsterdam, The Netherlands.
authors: M L
authors: ML
authors: Urbanus
authors: P A
authors: PA
authors: Scotti
authors: L
authors: L
authors: Froderberg
authors: A
authors: A
authors: Saaf
authors: J W
authors: JW
authors: de Gier
authors: J
authors: J
authors: Brunner
authors: J C
authors: JC
authors: Samuelson
authors: R E
authors: RE
authors: Dalbey
authors: B
authors: B
authors: Oudega
authors: J
authors: J
authors: Luirink
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism*; D002462:Cell Membrane / Q000201:enzymology* / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology / Q000378:metabolism; D029968:Escherichia coli Proteins; D050356:Lipid Metabolism; D008565:Membrane Proteins / Q000378:metabolism*; D026901:Membrane Transport Proteins; D008954:Models, Biological; D010957:Plasmids / Q000378:metabolism; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism; D000069816:SEC Translocation Channels; D000081416:SecA Proteins
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D029968:Escherichia coli Proteins; C068023:FtsQ protein, E coli; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D012333:RNA, Messenger; D000069816:SEC Translocation Channels; C057841:SecY protein, E coli; C412443:YIDC protein, E coli; D000251:Adenosine Triphosphatases; D000081416:SecA Proteins