Methodology for optimizing functional miniature proteins based on avian pancreatic polypeptide using phage display.
Synthetic genes for avian pancreatic polypeptide (aPP) and for the miniature DNA binding protein PPBR4 were cloned and expressed on the surface of M13 bacteriophage. We anticipate that these constructs will have utility optimizing the properties of miniature proteins based on aPP that result from our previously described protein grafting procedure.
Chin
J W
JW
Department of Chemistry, Yale University, New Haven, CT 06520-8107, USA.
Grotzfeld
R M
RM
Fabian
M A
MA
Schepartz
A
A
D000818:Animals; D017104:Bacteriophage M13 / Q000378:metabolism; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000138:chemical synthesis; D015195:Drug Design; D008722:Methods; D010191:Pancreatic Polypeptide / Q000737:chemistry*; D010446:Peptide Fragments / Q000737:chemistry*; D019151:Peptide Library; D017433:Protein Structure, Secondary; D013329:Structure-Activity Relationship
D004268:DNA-Binding Proteins; D010446:Peptide Fragments; D019151:Peptide Library; C009725:pancreatic polypeptide, avian; D010191:Pancreatic Polypeptide