A fibronectin-binding protein from rice bran with cell adhesion activity for animal tumor cells.
A rice bran 57-kDa protein was isolated by affinity chromatography with fibronectin immobilized on agarose. This fibronectin-binding protein designated as RB-57 had an amino-terminal amino acid sequence identical with that of a putative mature form of rice hydroxyproline-rich glycoprotein. A distinct feature of the amino acid composition of RB-57 was the high contents of hydroxyproline and proline representing about 45% of the total amino acids. The sugar analysis indicated that arabinose represented 46.8% of the total carbohydrates. RB-57 showed cell adhesion activity for murine Lewis lung carcinoma cells. The result suggests that RB-57 may play a role in plant cell adhesion, although cell adhesion-promoting activity for plant cells remains to be tested.
Shoji
Y
Y
School of Food and Nutritional Sciences, University of Shizuoka, Yada, Japan.
Mita
T
T
Isemura
M
M
Mega
T
T
Hase
S
S
Isemura
S
S
Aoyagi
Y
Y
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects*; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D005353:Fibronectins / Q000378:metabolism*; D009374:Neoplasms, Experimental / Q000473:pathology*; D012275:Oryza / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D000596:Amino Acids; D005353:Fibronectins; D010940:Plant Proteins