Reference intervals for the markers of proteinuria with a standardised bed-rest collection of urine.
Reference intervals for markers of proteinuria or glomerular charge selectivity were measured in 61 healthy female and 61 healthy male individuals. Timed bed-rest and daytime collections were used to assess significance of preanalytical variability of results. Bed-rest collections are advisable for research on renal damage, whereas in routine care, robust protein/creatinine ratios work as practical estimates of protein excretion rates, the correlations to excretion rates improving with increasing proteinuria. For glomerular charge selectivity, pancreatic/salivary isoamylase clearance ratio showed lower within-subject biological variation than IgG/IgG4 clearance ratio, allowing more accurate classification into normal and reduced charge selectivity. With our method, the lower 2.5% reference intervals for isoamylase clearance ratio were 1.1 in men and 1.9 in women.
Kouri
T
T
Tampereen Yliopistollinen Sairaala, Kliinisen kemian yksikkö, Tampere, Finland. timo.kouri@ppshp.fi
Harmoinen
A
A
Laurila
K
K
Ala-Houhala
I
I
Koivula
T
T
Pasternack
A
A
D000118:Acetylglucosaminidase / Q000652:urine*; D000293:Adolescent; D000328:Adult; D000510:Alpha-Globulins / Q000652:urine*; D000681:Amylases / Q000652:urine*; D001510:Bed Rest; D015415:Biomarkers / Q000652:urine*; D015992:Body Mass Index; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000652:urine*; D007527:Isoenzymes / Q000652:urine; D008297:Male; D008875:Middle Aged; D011507:Proteinuria / Q000652:urine*; D012016:Reference Values; D012463:Saliva / Q000201:enzymology; D013048:Specimen Handling
D000510:Alpha-Globulins; D015415:Biomarkers; D007074:Immunoglobulin G; D007527:Isoenzymes; C016001:alpha-1-microglobulin; D000681:Amylases; D000118:Acetylglucosaminidase