Constant expression of cyclooxygenase-2 gene in prostate and the lower urinary tract of estrogen-treated male rats.
Expression of cyclooxygenase-2 (E. C. 1.14.99.1) in prostate and the lower urinary tract (LUT) of the neonatally estrogenized male rat has been studied by using a COX-2's PCR fragment of 724 nt spanning 3 introns and a 478nt internal standard for quantitative RT-PCR. The same fragment of 724 nt was used for RNA probe in Northern hybridization. Neonatal estrogenization (10 microg/day of diethylstilbestrol on days 1-5) had no effect on COX-2 expression in prostatic urethra, prostatic lobes, or bladder. Acute estrogen treatment of castrated animals did not induce COX-2 expression, either. In addition the differential expression of basal level of COX-2 in the different lobes of prostate in normal rat was demonstrated. Our results suggest a constant expression of COX-2 gene in prostate and the lower urinary tract of the neonatally estrogenized (neoDES) rats. The present study indicates that the increased expression of COX-2 is probably not essential for the estrogen-driven development of stromal inflammation or hyperplastic and dysplastic alterations in the prostate of neoDES rats.
Luo
C
C
Department of Pediatrics, University of Turku, Finland. Cheng.Luo@utu.fi
Strauss
L
L
Ristimäki
A
A
Streng
T
T
Santti
R
R
D000818:Animals; D000831:Animals, Newborn; D051546:Cyclooxygenase 2; D004054:Diethylstilbestrol / Q000494:pharmacology*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects / Q000502:physiology*; D007527:Isoenzymes / Q000235:genetics*; D008297:Male; D009919:Orchiectomy; D011451:Prostaglandin-Endoperoxide Synthases / Q000235:genetics*; D011467:Prostate / Q000187:drug effects / Q000201:enzymology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014521:Urethra / Q000187:drug effects / Q000201:enzymology*; D001743:Urinary Bladder / Q000187:drug effects / Q000201:enzymology*; D014649:Vas Deferens / Q000187:drug effects / Q000201:enzymology
D007527:Isoenzymes; D004054:Diethylstilbestrol; D051546:Cyclooxygenase 2; D011451:Prostaglandin-Endoperoxide Synthases