[Generation of human recombinant antibody Fab fragment to influenza virus from combined antibody library].
OBJECTIVE
To acquire the recombinant human monoclonal antibodies to influenza virus which can be used in mucous membrane treatment to prevent the infection of virus in animal model in the future.


METHODS
Construct and pan human Fab antibody library to influenza virus Sydney strain (H3N2) from vaccinated donors by using phage display technology and secreted expression in E. Coli system. The positive Fab clones with neutralization activity to influenza virus were selected and characterized through HI test, IFA and VN test.


RESULTS
Separate two Fab clones, IV-2 and IV-6, appear positive responses in ELISA and IFA. These two clones can inhibit hemagglutination in HI test and can decrease viral titer by 30 and 20 times in viral neutralization assay, respectively.


CONCLUSIONS
We have got two clones of Fab fragments with neutralization activity to influenza virus Sydney strain (H3N2).
Yang
G
G
State Key Lab for Molecular Virology and Genetic Engineering, Institute of Virology, Chinese Academy of Preventive Medicine, Beijing 100052, China.
Han
F
F
Liang
M
M
D002478:Cells, Cultured; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000096:biosynthesis*; D009980:Influenza A virus / Q000276:immunology*; D011994:Recombinant Proteins / Q000096:biosynthesis
D007140:Immunoglobulin Fab Fragments; D011994:Recombinant Proteins