Disruption of thylakoid-associated kinase 1 leads to alteration of light harvesting in Arabidopsis.
To survive fluctuations in quality and intensity of light, plants and algae are able to preferentially direct the absorption of light energy to either one of the two photosystems, PSI or PSII. This rapid process is referred to as a state transition and has been correlated with the phosphorylation and migration of the light-harvesting complex protein (LHCP) between PSII and PSI. We show here that thylakoid protein kinases (TAKs) are required for state transitions in Arabidopsis. Antisense TAK1 expression leads to a loss of LHCP phosphorylation and a reduction in state transitions. Preferential activation of PSII causes LHCP to accumulate with PSI, and TAK1 mutants disrupt this process. Finally, TAKs also influence the phosphorylation of multiple thylakoid proteins.
Snyders
S
S
Department of Biology, Duke University, Durham, North Carolina 27708, USA.
Kohorn
B D
BD
D017360:Arabidopsis / Q000201:enzymology* / Q000502:physiology / Q000528:radiation effects*; D029681:Arabidopsis Proteins; D001483:Base Sequence; D003580:Cytochromes / Q000378:metabolism; D045348:Cytochromes f; D017931:DNA Primers; D008027:Light; D020930:MAP Kinase Kinase Kinases; D009154:Mutation; D010766:Phosphorylation; D045322:Photosynthetic Reaction Center Complex Proteins / Q000378:metabolism*; D011485:Protein Binding; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D000099151:MAP Kinase Kinase Kinase 7
D029681:Arabidopsis Proteins; D003580:Cytochromes; D045348:Cytochromes f; D017931:DNA Primers; D020930:MAP Kinase Kinase Kinases; D045322:Photosynthetic Reaction Center Complex Proteins; D011494:Protein Kinases; D011994:Recombinant Proteins; D000099151:MAP Kinase Kinase Kinase 7; C472828:PETA protein, Arabidopsis