Identification of goose (Anser anser) and mule duck (Anasplatyrhynchos x Cairina moschata) foie gras by multiplex polymerase chain reaction amplification of the 5S RDNA gene.
Polymerase chain reaction (PCR) amplification of the nuclear 5S rDNA gene has been used for the identification of goose and mule duck foie gras. Two species-specific reverse primers were designed and used in a multiplex reaction, together with a forward universal primer, to amplify specific fragments of the 5S rDNA in each species. The different sizes of the species-specific amplicons, separated by agarose gel electrophoresis, allowed clear identification of goose and mule duck foie gras samples. This genetic marker can be useful for detecting fraudulent substitution of the duck liver for the more expensive goose liver.
Rodríguez
M A
MA
Departamento de Nutrición y Bromatología III (Higiene y Tecnología de los Alimentos), Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain.
García
T
T
González
I
I
Asensio
L
L
Fernández
A
A
Lobo
E
E
Hernández
P E
PE
Martín
R
R
D000818:Animals; D001483:Base Sequence; D004275:DNA, Ribosomal / Q000235:genetics*; D004372:Ducks / Q000145:classification* / Q000235:genetics; D005234:Fatty Liver; D005777:Geese / Q000145:classification* / Q000235:genetics; D005784:Gene Amplification; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D011202:Poultry Products; D012341:RNA, Ribosomal, 5S / Q000235:genetics*
D004275:DNA, Ribosomal; D012341:RNA, Ribosomal, 5S