title: Entorhinal cortex projection cells to the hippocampal formation in vitro.
abstract: Retrogradely labeled cells in superficial and deep layers of the entorhinal cortex (EC) were analyzed following application of the fluorescent tracer rhodamine-dextran-amine in different sites of the hippocampal formation in a slice preparation. The results demonstrate a strong projection from layer IV/V to the dentate gyrus, that is in slices significantly stronger than that from layer II. In deep layers a large number of multipolar cells were found which were only labeled by dye application to the subiculum. Patch-clamp recordings from these cells revealed intrinsic low threshold membrane potential oscillations, suggesting their possible contribution to oscillatory network activity of the EC and subiculum.
authors: Johannes-Müller-Institute of Physiology at the Charité, Humboldt University, Berlin, Tucholskystrasse 2, 10117 Berlin, Germany.
authors: T
authors: T
authors: Dugladze
authors: U
authors: U
authors: Heinemann
authors: T
authors: T
authors: Gloveli
mesh_terms: D000818:Animals; D001369:Axons / Q000502:physiology / Q000648:ultrastructure*; D001683:Biological Clocks / Q000502:physiology; D048429:Cell Size / Q000502:physiology*; D003712:Dendrites / Q000502:physiology / Q000648:ultrastructure*; D003911:Dextrans / Q000493:pharmacokinetics; D018728:Entorhinal Cortex / Q000166:cytology* / Q000502:physiology; D005456:Fluorescent Dyes / Q000493:pharmacokinetics; D006624:Hippocampus / Q000166:cytology* / Q000502:physiology; D008239:Lysine / Q000031:analogs & derivatives / Q000494:pharmacology; D008564:Membrane Potentials / Q000502:physiology; D009434:Neural Pathways / Q000166:cytology* / Q000502:physiology; D009924:Organ Culture Techniques; D051381:Rats; D012235:Rhodamines / Q000493:pharmacokinetics
chemical_list: D003911:Dextrans; D005456:Fluorescent Dyes; D012235:Rhodamines; C005358:tetramethylrhodamine; C013411:biocytin; D008239:Lysine