Effect of arachidonic acid-rich oil on lipids and arachidonate metabolites in ethanol- treated rats.
The effects of dietary arachidonic acid-rich oil (AAoil) on lipids and arachidonate metabolites in the liver and plasma were evaluated in ethanol-treated rats. Rats were fed a purified diet containing 10% weight of lard or AAoil for 14 days. Ethanol was administered by gavage at a single daily dose of 3 g/kg body weight. Comparing with the lard group, a decrease was observed in liver fatty vacuoles in the AAoil group. Plasma 6-keto-prostaglandin (PG) F1 alpha and thromboxane (TX) B(2)levels and the 6-keto-PGF1 alpha/TXB(2)ratio increased significantly in the AAoil group. Liver 6-keto-PGF1 alpha also increased but not leukotriene B(4)in the AAoil group. In the phospholipid fraction of liver tissue, plasma and red blood cells, arachidonic acid (20:4n-6) and docosatetraenoic acid (22:4n-6) increased and oleic acid (18:1n-9) and linoleic acid (18:2n-6) decreased significantly in the AAoil group compared with the lard group. These observations suggest that AAoil supplementation reduces liver injury of ethanol-treated rats, although longer observation will be necessary for confirmation.
Okita
M
M
Department of Nutritional Science, Okayama Prefectural University, Soja, 719-1197, Japan. okita@fhw.oka-pu.ac.jp
Sasagawa
T
T
Ohta
Y
Y
Kaneyuki
T
T
Suzuki
K
K
D015121:6-Ketoprostaglandin F1 alpha / Q000097:blood; D000818:Animals; D016718:Arachidonic Acid / Q000097:blood / Q000378:metabolism* / Q000494:pharmacology*; D001835:Body Weight / Q000187:drug effects; D004883:Erucic Acids / Q000378:metabolism; D004912:Erythrocytes / Q000378:metabolism; D000431:Ethanol / Q000494:pharmacology*; D005227:Fatty Acids / Q000378:metabolism; D007975:Leukotriene B4 / Q000378:metabolism; D019787:Linoleic Acid / Q000378:metabolism; D050356:Lipid Metabolism; D008055:Lipids / Q000097:blood; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008297:Male; D019301:Oleic Acid / Q000378:metabolism; D009929:Organ Size / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D017392:Thiobarbituric Acid Reactive Substances; D013929:Thromboxane B2 / Q000097:blood / Q000378:metabolism; D013997:Time Factors
D004883:Erucic Acids; D005227:Fatty Acids; D008055:Lipids; D017392:Thiobarbituric Acid Reactive Substances; D007975:Leukotriene B4; D016718:Arachidonic Acid; D019301:Oleic Acid; D000431:Ethanol; D013929:Thromboxane B2; D015121:6-Ketoprostaglandin F1 alpha; D019787:Linoleic Acid