title: Differential gene expression in a murine model of cancer cachexia.
abstract: Murine adenocarcinoma 16 (MAC16) tumors and cell lines induce cachexia in NMRI nude mice, whereas histologically similar MAC13 tumors do not. After confirming these findings in BALB/c nude mice, we demonstrated that this tissue wasting was not related to decreased food intake or increased total body oxidative metabolism. Previous studies have suggested that MAC16's cachexigenic properties may involve the production of tumor-specific factors. We therefore screened for genes having increased expression in the MAC16 compared with the MAC13 cell line by performing hybridization to a murine cDNA expression array, by generation and comparison of cDNA libraries from each cell line, and by PCR-based subtractive hybridization. Northern blot hybridization was performed to confirm differences in transcript expression. Transcripts encoding insulin-like growth factor binding protein-4, cathepsin B, ferritin light and heavy chain, endogenous long-terminal repeat sequences, and a viral envelope glycoprotein demonstrated increased expression in the MAC16 cell line. The roles of a number of these genes in known metabolic pathways identify them as potential participants in the induction of cachexia.
authors: Department of Anesthesiology and Critical Care Medicine, The Johns Hopkins Hospital, Baltimore, Maryland 21287, USA. cmonitto@welchlink.welch.jhu.edu
authors: C L
authors: CL
authors: Monitto
authors: D
authors: D
authors: Berkowitz
authors: K M
authors: KM
authors: Lee
authors: S
authors: S
authors: Pin
authors: D
authors: D
authors: Li
authors: M
authors: M
authors: Breslow
authors: B
authors: B
authors: O'Malley
authors: M
authors: M
authors: Schiller
mesh_terms: D000230:Adenocarcinoma / Q000150:complications / Q000235:genetics*; D000818:Animals; D015152:Blotting, Northern; D002100:Cachexia / Q000209:etiology / Q000235:genetics*; D002401:Cathepsin B / Q000096:biosynthesis / Q000235:genetics; D002999:Clone Cells; D003110:Colonic Neoplasms / Q000150:complications / Q000235:genetics*; D004195:Disease Models, Animal; D020077:Endogenous Retroviruses / Q000235:genetics; D005293:Ferritins / Q000096:biosynthesis / Q000235:genetics; D020869:Gene Expression Profiling; D015723:Gene Library; D018974:Insulin-Like Growth Factor Binding Protein 4 / Q000096:biosynthesis / Q000235:genetics; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis; D014407:Tumor Cells, Cultured; D015854:Up-Regulation
chemical_list: D018974:Insulin-Like Growth Factor Binding Protein 4; D012333:RNA, Messenger; D005293:Ferritins; D002401:Cathepsin B