Apoptotic cells overexpress vinculin and induce vinculin-specific cytotoxic T-cell cross-priming.
Here we show that apoptotic cells overexpress vinculin and are ingested by dendritic cells, which subsequently cross-prime vinculin-specific cytotoxic T lymphocytes (CTLs). Successful cross-priming requires that the apoptotic cells provide maturation signals to dendritic cells through CD40-CD40 ligand (CD40L) interactions. If apoptotic cells are CD40L-, the help of a third T cell is needed for priming, indicating a regulatory role for apoptotic cells in determining priming or tolerance. Vinculin-specific CTL priming is also related to apoptosis in vivo, given that in HIV-seropositive individuals, the frequency of specific CTLs depends on the proportion of peripheral CD40L+ apoptotic cells.
Propato
A
A
Fondazione Andrea Cesalpino, Dipartimento di Medicina Interna, Università degli Studi di Roma La Sapienza, Rome, Italy.
Cutrona
G
G
Francavilla
V
V
Ulivi
M
M
Schiaffella
E
E
Landt
O
O
Dunbar
R
R
Cerundolo
V
V
Ferrarini
M
M
Barnaba
V
V
D000595:Amino Acid Sequence; D017209:Apoptosis; D001483:Base Sequence; D002460:Cell Line; D003429:Cross Reactions / Q000276:immunology*; D017931:DNA Primers; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D016596:Vinculin / Q000737:chemistry / Q000378:metabolism* / Q000502:physiology
D017931:DNA Primers; D016596:Vinculin