title: Design of functionalized lipids and evidence for their binding to photosystem II core complex by oxygen evolution measurements, atomic force microscopy, and scanning near-field optical microscopy.
abstract: Photosystem II core complex (PSII CC) absorbs light energy and triggers a series of electron transfer reactions by oxidizing water while producing molecular oxygen. Synthetic lipids with different alkyl chains and spacer lengths bearing functionalized headgroups were specifically designed to bind the Q(B) site and to anchor this large photosynthetic complex (240 kDa) in order to attempt two-dimensional crystallization. Among the series of different compounds that have been tested, oxygen evolution measurements have shown that dichlorophenyl urea (DCPU) binds very efficiently to the Q(B) site of PSII CC, and therefore, that moiety has been linked covalently to the headgroup of synthetic lipids. The analysis of the monolayer behavior of these DCPU-lipids has allowed us to select ones bearing long spacers for the anchoring of PSII CC. Oxygen evolution measurements demonstrated that these long-spacer DCPU-lipids specifically bind to PSII CC and inhibit electron transfer. With the use of atomic force microscopy (AFM) and scanning near-field optical microscopy (SNOM), it was possible to visualize domains of PSII CC bound to DCPU-lipid monolayers. SNOM imaging has enabled us to confirm that domains observed by AFM were composed of PSII CC. Indeed, the SNOM topography images presented similar domains as those observed by AFM, but in addition, it allowed us to determine that these domains are fluorescent. Electron microscopy of these domains, however, has shown that the bound PSII CC was not crystalline.
authors: Département de Chimie-Biologie, Université du Québec à Trois-Rivières, Trois-Rivières, Québec G9A 5H7, Canada.
authors: E
authors: E
authors: Trudel
authors: J
authors: J
authors: Gallant
authors: S
authors: S
authors: Mons
authors: C
authors: C
authors: Mioskowski
authors: L
authors: L
authors: Lebeau
authors: K
authors: K
authors: Jeuris
authors: P
authors: P
authors: Foubert
authors: F
authors: F
authors: De Schryver
authors: C
authors: C
authors: Salesse
mesh_terms: D004237:Diuron / Q000737:chemistry / Q000378:metabolism; D006540:Herbicides / Q000737:chemistry / Q000378:metabolism; D050356:Lipid Metabolism; D008055:Lipids / Q000138:chemical synthesis / Q000737:chemistry*; D008853:Microscopy / Q000379:methods; D018625:Microscopy, Atomic Force; D008958:Models, Molecular; D008968:Molecular Conformation; D010100:Oxygen / Q000378:metabolism*; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D045332:Photosystem II Protein Complex; D011485:Protein Binding; D018724:Spinacia oleracea
chemical_list: D006540:Herbicides; D008055:Lipids; D045322:Photosynthetic Reaction Center Complex Proteins; D045332:Photosystem II Protein Complex; D004237:Diuron; D010100:Oxygen