title: Use of temporal temperature gradient gel electrophoresis to identify flaA and fim3 sequence types in Bordetella bronchiseptica.
abstract: OBJECTIVE
  The aim of this study was to develop an approach to detect variation in the flaA and fim3 genes amongst animal isolates of Bordetella bronchiseptica using temporal temperature gradient gel electrophoresis (TTGE).
  
  
  RESULTS
  Amplicons representing three flaA and two fim3 sequence types were subjected to TTGE analysis. It was possible to distinguish clearly between each of the sequence types using TTGE.
  
  
  CONCLUSIONS
  This suggests that TTGE could be a useful tool for studying the epidemiology of B. bronchiseptica.
  
  
  CONCLUSIONS
  PCR amplification coupled to TTGE offers a general method for the rapid screening of large numbers of microbial strains for variations in gene sequences.
authors: Department of Medical Microbiology and Genito-Urinary Medicine, University of Liverpool, UK.
authors: A
authors: A
authors: Shina
authors: S K
authors: SK
authors: Mon
authors: S
authors: S
authors: Dawson
authors: R M
authors: RM
authors: Gaskell
authors: C A
authors: CA
authors: Hart
authors: C
authors: C
authors: Winstanley
mesh_terms: D000828:Animal Population Groups; D000818:Animals; D000942:Antigens, Bacterial; D001426:Bacterial Proteins / Q000235:genetics* / Q000302:isolation & purification; D001885:Bordetella Infections / Q000453:epidemiology / Q000382:microbiology; D016950:Bordetella bronchiseptica / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D036781:Fimbriae Proteins; D005408:Flagellin / Q000235:genetics* / Q000302:isolation & purification; D014644:Genetic Variation; D016133:Polymerase Chain Reaction / Q000379:methods; D013696:Temperature; D010566:Virulence Factors, Bordetella
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D010566:Virulence Factors, Bordetella; C101542:fim3 protein, Bordetella; D005408:Flagellin; C067138:flaA protein, bacteria; D036781:Fimbriae Proteins