Phenylethanolamine N-methyltransferase kinetics: bovine versus recombinant human enzyme.
Epinephrine (Epi) acts as a neurotransmitter in the brain, but its function therein is not well understood. Phenylethanolamine N-methyltransferase (PNMT) catalyzes the final step in the biosynthesis of Epi and is thus a pharmacological target to investigate the function of Epi in the central nervous system. The kinetic differences between bovine adrenal PNMT and human brain PNMT for a number of substrates and inhibitors are examined and the results reported.
Grunewald
G L
GL
Department of Medicinal Chemistry, University of Kansas, 4060 Malott Hall, 1251 Wescoe Hall Drive, Lawrence, KS 66045-7582, USA. ggrunewald@ukans.edu
McLeish
M J
MJ
Criscione
K R
KR
D000818:Animals; D002417:Cattle; D004837:Epinephrine; D006801:Humans; D007700:Kinetics; D008958:Models, Molecular; D010625:Phenylethanolamine N-Methyltransferase / Q000737:chemistry / Q000378:metabolism*; D011994:Recombinant Proteins; D013237:Stereoisomerism; D013329:Structure-Activity Relationship; D013379:Substrate Specificity
D011994:Recombinant Proteins; D010625:Phenylethanolamine N-Methyltransferase; D004837:Epinephrine