title: Detection of immune system cells in paraffin wax-embedded ovine tissues.
abstract: This report describes a method (fixation, paraffin wax-embedding and immunolabelling) for the demonstration of several immune system cell epitopes (CD1, CD2, CD4, CD8, CD14, CD21, CD45R, WC-1, 28 kDa surface antigen, immunoglobulins and MHC II antigens) in ovine lymph nodes collected at necropsy. Cell surface epitopes considered to be sensitive to processing methods were successfully demonstrated by a procedure that included the use of a non-aldehyde-containing, zinc salts-based fixative, coupled with a sensitive system of immunolabelling. This novel method had the advantage of avoiding antigen-retrieval steps and of providing consistently good morphological definition.
authors: Moredun Research Institute, Pentlands Science Park, Penicuik, Midlothian, EH26 0PZ, UK.
authors: L
authors: L
authors: González
authors: I
authors: I
authors: Anderson
authors: D
authors: D
authors: Deane
authors: C
authors: C
authors: Summers
authors: D
authors: D
authors: Buxton
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D015703:Antigens, CD / Q000032:analysis*; D000939:Epitopes / Q000032:analysis; D019084:Fluorescent Antibody Technique, Indirect / Q000662:veterinary; D007124:Immunoenzyme Techniques / Q000662:veterinary; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology*; D016612:Paraffin Embedding / Q000662:veterinary*; D012756:Sheep / Q000276:immunology*; D014021:Tissue Preservation / Q000662:veterinary*
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000939:Epitopes