Labeling patterns of chloroplastidic isoprenoids in cultured cells of liverwort Ptychanthus striatus.
Incorporation studies administering 2H- and 13C-labeled mevalonate (MVA) and 13C-labeled glucose to suspension cultured cells of the liverwort, Ptychanthus striatus, were carried out in order to examine the biosynthesis of the phytyl side-chain of chlorophyll a. Administration of 13C- and 2H-labeled MVA provided evidence for the involvement of the MVA pathway in the phytyl side-chain biosynthesis and preferential labeling of the farnesyl diphosphate (FPP)-derived portion. An alternate labeling pattern in the phytyl side-chain was observed which was slightly different to the non-equivalent labeling in other liverworts, such as Heteroscyphus planus and Lophocolea heterophylla and in the hornwort, Anthoceros punctatus. The labeling pattern observed after the administration of 13C-labeled glucose revealed the simultaneous involvement of the non-MVA pathway in the phytol biosynthesis of P. striatus cells.
Karunagoda
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RP
The United Graduate School of Agricultural Science, Iwate University, Morioka, Japan.
Itoh
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D
Katoh
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Nabeta
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K
D000107:Acetylation; D002478:Cells, Cultured; D002736:Chloroplasts / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D008798:Mevalonic Acid / Q000378:metabolism; D015394:Molecular Structure; D059828:Plant Cells; D010944:Plants / Q000378:metabolism*; D011106:Polyisoprenyl Phosphates / Q000378:metabolism*; D012717:Sesquiterpenes
D011106:Polyisoprenyl Phosphates; D012717:Sesquiterpenes; C004808:farnesyl pyrophosphate; D005947:Glucose; D008798:Mevalonic Acid