title: Molecular dynamics of point mutated I-A(k) molecules expressed on lymphocytes.
abstract: We have recently reported the lateral and rotational diffusion parameters for I-A(k) molecules expressing various cytoplasmic truncations (Int. Immunol. 12 (2000) 1319). We now describe the membrane dynamics of I-A(k) with various mutations in the presumed contact region between alphabeta-heterodimers in an (alphabeta)2 dimer of dimers structure. Such mutations are known to strongly affect the antigen presentation ability of these molecules (Int. Immunol. 10 (1998) 1237-1249) but cause relatively small changes in the molecular dynamics of I-A(k). Lateral diffusion coefficients of I-A(k) wild-type molecules and mutants obtained via fringe fluorescence photobleaching recovery (FPR) ranged from 1.1 to 2.3x10(-10)cm2/s at room temperature while fractional mobilities averaged 75+/-6%. For all cell types examined, treatment with either hen egg lysozyme 46-61 peptide or db-cAMP reduced the I-A(k) mobile fraction by about 10% relative to untreated cells, suggesting that these treatments may increase lateral confinement of class II in lipid rafts or cytoskeletal interactions of the molecules. Wild-type I-A(k) and mutants capable of normal or partial antigen presentation exhibited, as a group, slightly longer rotational correlation times (RCT) at 4 degrees C than did mutants inactive in antigen presentation, 14+/-4 versus 10+/-1 micros, respectively. Moreover, peptide, cAMP and anti-CD40 mAb treatment all increased rotational correlation times for fully- and partially-functional I-A(k) but not for non-functional molecules. For example, 16 h peptide treatment yielded average RCTs of 28+/-12 and 10+/-1 micros for the groups of functional and non-functional molecules, respectively. Such modulation of the dynamics of functional class II molecules is consistent with these treatments' stabilization of class II or induction of new gene expression. Measurements of fluorescence resonant energy transfer between I-A(k), though complicated by cellular autofluorescence, averaged 6+/-7% over 15 cells or treatments, a result consistent with the presence of a small fraction of I-A(k) as a dimer of dimers species. In summary, our results suggest subtle changes in the molecular motions of class II molecules correlate with a significant impact on class II function. Molecules active in antigen presentation exhibit more restricted motion in the membrane, and thus presumably more extensive intermolecular interactions, than non-functional molecules. Further, treatments, such as db-cAMP and anti-CD40, which rescue antigen presentation by partially defective mutants, appear to increase such interactions, several types of which have already been reported for class II. A more detailed understanding of these phenomena will require both more sensitive biophysical tools and a more refined model of the role of class II intermolecular interactions in antigen presentation.
authors: Department of Chemistry, Colorado State University, Fort Collins, CO 80523, USA.
authors: H M
authors: HM
authors: Munnelly
authors: C J
authors: CJ
authors: Brady
authors: G M
authors: GM
authors: Hagen
authors: R D
authors: RD
authors: Horvat
authors: W F
authors: WF
authors: Wade
authors: D A
authors: DA
authors: Roess
authors: B G
authors: BG
authors: Barisas
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D017951:Antigen Presentation; D001402:B-Lymphocytes / Q000276:immunology*; D003994:Bucladesine / Q000494:pharmacology; D019013:CD40 Antigens / Q000276:immunology; D004058:Diffusion; D019281:Dimerization; D004735:Energy Transfer; D005453:Fluorescence; D000949:Histocompatibility Antigens Class II / Q000737:chemistry* / Q000235:genetics / Q000276:immunology*; D051379:Mice; D009113:Muramidase / Q000494:pharmacology; D010446:Peptide Fragments / Q000494:pharmacology; D017354:Point Mutation; D012399:Rotation; D014407:Tumor Cells, Cultured
chemical_list: D000911:Antibodies, Monoclonal; D019013:CD40 Antigens; D000949:Histocompatibility Antigens Class II; C111388:I-Ak antigen; D010446:Peptide Fragments; C066759:hen egg lysozyme peptide (46-61); D003994:Bucladesine; D009113:Muramidase