title: Apoptotic cells overexpress vinculin and induce vinculin-specific cytotoxic T-cell cross-priming.
abstract: Here we show that apoptotic cells overexpress vinculin and are ingested by dendritic cells, which subsequently cross-prime vinculin-specific cytotoxic T lymphocytes (CTLs). Successful cross-priming requires that the apoptotic cells provide maturation signals to dendritic cells through CD40-CD40 ligand (CD40L) interactions. If apoptotic cells are CD40L-, the help of a third T cell is needed for priming, indicating a regulatory role for apoptotic cells in determining priming or tolerance. Vinculin-specific CTL priming is also related to apoptosis in vivo, given that in HIV-seropositive individuals, the frequency of specific CTLs depends on the proportion of peripheral CD40L+ apoptotic cells.
authors: Fondazione Andrea Cesalpino, Dipartimento di Medicina Interna, Università degli Studi di Roma La Sapienza, Rome, Italy.
authors: A
authors: A
authors: Propato
authors: G
authors: G
authors: Cutrona
authors: V
authors: V
authors: Francavilla
authors: M
authors: M
authors: Ulivi
authors: E
authors: E
authors: Schiaffella
authors: O
authors: O
authors: Landt
authors: R
authors: R
authors: Dunbar
authors: V
authors: V
authors: Cerundolo
authors: M
authors: M
authors: Ferrarini
authors: V
authors: V
authors: Barnaba
mesh_terms: D000595:Amino Acid Sequence; D017209:Apoptosis; D001483:Base Sequence; D002460:Cell Line; D003429:Cross Reactions / Q000276:immunology*; D017931:DNA Primers; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D016596:Vinculin / Q000737:chemistry / Q000378:metabolism* / Q000502:physiology
chemical_list: D017931:DNA Primers; D016596:Vinculin