Use of Percoll for the infection of cells in vitro with Cryptosporidium parvum oocysts.
A method for the infection of non-adherent THP-1 cells and adherent MDBK cells with Cryptosporidium parvum oocysts using isotonic Percoll solutions was developed. Excystation was maximal after 2 h, but toxicity increased with the oocyst/cell ratio and the incubation time. The infection rates did not increase with the oocyst/cell ratio and both cell types were equally parasitized.
Lawton
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P
Département Pharmaceutique de Parasitologie et Mycologie Médicale, Faculté de Pharmacie, Université Claude-Bernard, 8 avenue Rockefeller, F-69373 Cedex 08, Lyon, France. lawton@univ-lyon1.fr
Mancassola
R
R
Naciri
M
M
Pétavy
A F
AF
D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D016785:Cryptosporidium parvum / Q000254:growth & development* / Q000472:pathogenicity; D066298:In Vitro Techniques; D010063:Ovum / Q000254:growth & development; D010270:Parasite Egg Count; D011205:Povidone; D012822:Silicon Dioxide
C016039:Percoll; D012822:Silicon Dioxide; D011205:Povidone