Use of temporal temperature gradient gel electrophoresis to identify flaA and fim3 sequence types in Bordetella bronchiseptica.
OBJECTIVE
The aim of this study was to develop an approach to detect variation in the flaA and fim3 genes amongst animal isolates of Bordetella bronchiseptica using temporal temperature gradient gel electrophoresis (TTGE).


RESULTS
Amplicons representing three flaA and two fim3 sequence types were subjected to TTGE analysis. It was possible to distinguish clearly between each of the sequence types using TTGE.


CONCLUSIONS
This suggests that TTGE could be a useful tool for studying the epidemiology of B. bronchiseptica.


CONCLUSIONS
PCR amplification coupled to TTGE offers a general method for the rapid screening of large numbers of microbial strains for variations in gene sequences.
Shina
A
A
Department of Medical Microbiology and Genito-Urinary Medicine, University of Liverpool, UK.
Mon
S K
SK
Dawson
S
S
Gaskell
R M
RM
Hart
C A
CA
Winstanley
C
C
D000828:Animal Population Groups; D000818:Animals; D000942:Antigens, Bacterial; D001426:Bacterial Proteins / Q000235:genetics* / Q000302:isolation & purification; D001885:Bordetella Infections / Q000453:epidemiology / Q000382:microbiology; D016950:Bordetella bronchiseptica / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D036781:Fimbriae Proteins; D005408:Flagellin / Q000235:genetics* / Q000302:isolation & purification; D014644:Genetic Variation; D016133:Polymerase Chain Reaction / Q000379:methods; D013696:Temperature; D010566:Virulence Factors, Bordetella
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D010566:Virulence Factors, Bordetella; C101542:fim3 protein, Bordetella; D005408:Flagellin; C067138:flaA protein, bacteria; D036781:Fimbriae Proteins