Congruent phylogenies of most common small-subunit rRNA and dissimilatory sulfite reductase gene sequences retrieved from estuarine sediments.
The diversity of sulfate-reducing bacteria (SRB) in brackish sediment was investigated using small-subunit rRNA and dissimilatory sulfite reductase (DSR) gene clone libraries and cultivation. The phylogenetic affiliation of the most commonly retrieved clones for both genes was strikingly similar and produced Desulfosarcina variabilis-like sequences from the inoculum but Desulfomicrobium baculatum-like sequences from a high dilution in natural media. Related organisms were subsequently cultivated from the site. PCR bias appear to be limited (or very similar) for the two primersets and target genes. However, the DSR primers showed a much higher phylogenetic specificity. DSR gene analysis is thus a promising and specific approach for investigating SRB diversity in complex habitats.
Joulian
C
C
Institute of Biological Sciences, Department of Microbial Ecology, Aarhus University, Ny Munkegade, DK-8000 Aarhus C, Denmark. catherine.joulian@biology.aau.dk
Ramsing
N B
NB
Ingvorsen
K
K
D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D020564:Deltaproteobacteria / Q000145:classification* / Q000201:enzymology / Q000235:genetics; D004586:Electrophoresis / Q000379:methods; D005798:Genes, Bacterial; D020459:Genes, rRNA; D019015:Geologic Sediments / Q000382:microbiology*; D008969:Molecular Sequence Data; D050862:Oxidoreductases Acting on Sulfur Group Donors / Q000235:genetics*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012335:RNA, Ribosomal / Q000235:genetics; D012623:Seawater / Q000382:microbiology; D017422:Sequence Analysis, DNA; D016999:Sulfur-Reducing Bacteria / Q000145:classification* / Q000201:enzymology / Q000235:genetics
D004269:DNA, Bacterial; D012335:RNA, Ribosomal; D050862:Oxidoreductases Acting on Sulfur Group Donors