A novel C1-using denitrifier alcaligenes sp. STC1 and its genes for copper-containing nitrite reductase and azurin.
A novel denitrifier Alcaligenes sp. STC1 was identified. The strain efficiently denitrifies under an atmosphere of 10% oxygen (O2) where Paracoccus denitrificans, one of the most studied aerobic denitrifiers, had less denitrifying activity, indicating that the strain has an O2-torelant denitrifying system. It denitrified by using C1-carbon sources such as formate and methanol as well as glucose, glycerol, and succinate. The genes for the copper-containing nitrite reductase and azurin of this C1-using denitrifier were cloned. Their predicted products of them were similar to those of their counterparts and the maximum similarities were 90% and 92%, respectively.
Ozeki
S
S
Institute of Applied Biochemistry, University of Tsukuba, Ibaraki, Japan.
Baba
I
I
Takaya
N
N
Shoun
H
H
D000421:Alcaligenes / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D001400:Azurin / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D002712:Chlorides / Q000378:metabolism*; D003300:Copper / Q000378:metabolism*; D017931:DNA Primers; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009572:Nitrite Reductases / Q000737:chemistry / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
D002712:Chlorides; D017931:DNA Primers; D001400:Azurin; D003300:Copper; D009572:Nitrite Reductases