Migration pathways and behavior of glial progenitors in the postnatal forebrain.
The postnatal subventricular zone (SVZ) gives rise to many of the glial cells in the forebrain. We investigated migration pathways and dynamics of motility of progenitors from the neonatal rat forebrain SVZ by labeling progenitors in vivo with a retrovirus encoding green fluorescent protein (GFP) and then visualizing the dynamics of their movements by time-lapse fluorescence microscopy in slice preparations. Cells within the dorso-lateral SVZ moved in an apparently undirected fashion, but migrated in a directed manner after emigration into white matter and cortex, displaying both radial and tangential migration. Cells in the striatal-SVZ, a region of SVZ along the lateral wall of the ventricle, migrated parallel to the ventricular surface, and entered the striatum, where they migrated both perpendicular and parallel to the ventricular surface. Sometimes, cells in all these regions reversed their migration back toward the SVZ. Migration involved either elongation of the leading process followed by a quick translocation of the nucleus or a synchronous advancement of the nucleus and the leading process. Two distinct patterns of cellular changes were observed at orthogonal turning: one involves the cessation of cell body movement and the formation of a new leading process, and the other involves continuous cell body movement and bending of the leading process. The dynamic behavior of progenitors may reflect local tissue architecture and contribute to the widespread distribution of glia.
Kakita
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Department of Pathology, Brain Disease Research Center, Brain Research Institute, Niigata University. kakita@bri.niigata-u.ac.jp
D000818:Animals; D000831:Animals, Newborn; D002465:Cell Movement; D002478:Cells, Cultured; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins; D018613:Microscopy, Confocal; D009457:Neuroglia / Q000502:physiology*; D016548:Prosencephalon / Q000166:cytology* / Q000254:growth & development; D051381:Rats; D017207:Rats, Sprague-Dawley; D012190:Retroviridae; D013234:Stem Cells / Q000502:physiology*; D013997:Time Factors
D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins