Elisa to quantify hexanal-protein adducts in a meat model system.
Monoclonal antibodies (MAb) were produced to hexanal-bovine serum albumin conjugates. An indirect competitive ELISA was developed with a detection range of 1-50 ng of hexanal/mL. Hexanal conjugated to three different proteins was recognized, whereas free hexanal and the native proteins were not detected. The antibody cross-reacted with pentanal, heptanal, and 2-trans-hexenal conjugated to chicken serum albumin (CSA) with cross-reactivities of 37.9, 76.6, and 45.0%, respectively. There was no cross-reactivity with propanal, butanal, octanal, and nonanal conjugated to CSA. The hexanal content of a meat model system was determined using MAb and polyclonal antibody-based ELISAs and compared with analysis by a dynamic headspace gas chromatographic (HS-GC) method and a thiobarbituric acid reactive substances (TBARS) assay. Both ELISAs showed strong correlations with the HS-GC and TBARS methods. ELISAs may be a fast and simple alternative to GC for monitoring lipid oxidation in meat.
Zielinski
T L
TL
Department of Food Science and Human Nutrition, Michigan State University, East Lansing, MI 48824, USA.
Smith
S A
SA
Pestka
J J
JJ
Gray
J I
JI
Smith
D M
DM
D000447:Aldehydes / Q000302:isolation & purification*; D000818:Animals; D000911:Antibodies, Monoclonal; D002849:Chromatography, Gas; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D050356:Lipid Metabolism; D008460:Meat / Q000032:analysis; D010084:Oxidation-Reduction; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis
D000447:Aldehydes; D000911:Antibodies, Monoclonal; D017392:Thiobarbituric Acid Reactive Substances; C010463:n-hexanal