title: Purinergic-independent calcium signaling mediates recovery from hepatocellular swelling: implications for volume regulation.
abstract: Swelling of hepatocytes and other epithelia activates volume-sensitive ion channels that facilitate fluid and electrolyte efflux to restore cell volume, but the responsible signaling pathways are incompletely defined. Previous work in model HTC rat hepatoma cells has indicated that swelling elicits ATP release, which stimulates P2 receptors and activates Cl(-) channels, and that this mechanism is essential for hepatocellular volume recovery. Since P2 receptors are generally coupled to Ca(2+) signaling pathways, we determined whether hepatocellular swelling affected cytosolic [Ca(2+)], and if this involved a purinergic mechanism. Exposure of HTC cells to hypotonic media evoked an increase in cytosolic [Ca(2+)], which was followed by activation of K(+) and Cl(-) currents. Maneuvers that interfered with swelling-induced increases in cytosolic [Ca(2+)], including extracellular Ca(2+) removal and intracellular Ca(2+) store depletion with thapsigargin, inhibited activation of membrane currents and volume recovery. However, the swelling-induced increases in cytosolic [Ca(2+)] were unaffected by either extracellular ATP depletion with apyrase or blockade of P2 receptors with suramin. These findings indicate that swelling elicits an increase in hepatocellular Ca(2+), which is essential for ion channel activation and volume recovery, but that this increase does not stem from activation of volume-sensitive P2 receptors. Collectively, these observations imply that regulatory responses to hepatocellular swelling involve a dual requirement for a purinergic-independent Ca(2+) signaling cascade and a Ca(2+)-independent purinergic signaling pathway.
authors: Departments of Medicine and Pharmacology, University of Vermont College of Medicine, Burlington, Vermont 05401, USA.
authors: M W
authors: MW
authors: Roe
authors: A L
authors: AL
authors: Moore
authors: S D
authors: SD
authors: Lidofsky
mesh_terms: D000255:Adenosine Triphosphate / Q000502:physiology*; D000818:Animals; D002118:Calcium / Q000502:physiology*; D015220:Calcium Channels / Q000502:physiology; D048429:Cell Size; D022781:Hepatocytes / Q000502:physiology*; D053496:Inositol 1,4,5-Trisphosphate Receptors; D015221:Potassium Channels / Q000502:physiology; D051381:Rats; D018160:Receptors, Cytoplasmic and Nuclear / Q000502:physiology; D018048:Receptors, Purinergic P2 / Q000502:physiology; D014407:Tumor Cells, Cultured
chemical_list: D015220:Calcium Channels; D053496:Inositol 1,4,5-Trisphosphate Receptors; D015221:Potassium Channels; D018160:Receptors, Cytoplasmic and Nuclear; D018048:Receptors, Purinergic P2; D000255:Adenosine Triphosphate; D002118:Calcium