Molecular analysis of p53 tumor-suppressor gene and microsatellites in preneoplastic and neoplastic lesions of the colon and esophagus.
Mutations in exons 4-8 of the p53 gene by the PCR-SSCP analysis in preneoplastic and neoplastic lesions of the colon (n=11) and esophagus (n=18) were screened. p53 overexpression by immunohistochemistry in 11 colonic lesions and 13 microsatellites, in all the patients (n=29), were also studied. A positive result concordancy between the three techniques was found in 1 adenoma and 2 adenocarcinomas of the colon, each with loss of heterozygocity of microsatellites. Metaplastic lesions of esophagus showed biallelic mutations and low frequency of microsatellite alterations. The relationship between genetic alterations in p53, microsatellites and type of colon and esophageal lesions is discussed.
Copelli
S B
SB
Laboratorio de Biologia Molecular, Servicio de Patologia, Instituto de Estudios Oncologicos, Fundacion Maissa, Academia Nacional de Medicina de Buenos Aires, Av. Las Heras 3092, 1425 Buenos Aires, Argentina. sic@caece.edu.ar
Mazzeo
C
C
Gimenez
A
A
Casco
C
C
Meiss
R
R
Rubio
H
H
Speroni
A
A
Vanzulli
S
S
D000230:Adenocarcinoma / Q000209:etiology / Q000235:genetics* / Q000378:metabolism; D000236:Adenoma / Q000209:etiology / Q000235:genetics* / Q000378:metabolism; D003110:Colonic Neoplasms / Q000209:etiology / Q000235:genetics* / Q000378:metabolism; D004252:DNA Mutational Analysis; D004273:DNA, Neoplasm / Q000032:analysis; D004938:Esophageal Neoplasms / Q000209:etiology / Q000235:genetics* / Q000378:metabolism; D005260:Female; D016147:Genes, Tumor Suppressor / Q000235:genetics*; D016158:Genes, p53; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*; D008875:Middle Aged; D009154:Mutation; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis / Q000235:genetics*
D004273:DNA, Neoplasm; D016159:Tumor Suppressor Protein p53