title: Saccharomyces cerevisiae Adr1p governs fatty acid beta-oxidation and peroxisome proliferation by regulating POX1 and PEX11.
abstract: Saccharomyces cerevisiae Adr1p is essential for fatty acid degradation and peroxisome proliferation. Here, the role of Adr1p was examined with respect to the transcriptional regulation of the Pip2p-Oaf1p dependent genes POX1 and PEX11. POX1 encodes the rate-limiting enzyme of peroxisomal beta-oxidation, acyl-CoA oxidase. The POX1 promoter was shown to contain a canonical Adr1p element (UAS1), within which the oleate response element (ORE) was nested. PEX11 codes for a peroxin that is critical for normal peroxisome proliferation, and its promoter was shown similarly to contain a UAS1-like element overlapping the ORE. Northern analysis demonstrated that transcriptional up-regulation of both POX1 and PEX11 was abolished in adr1 Delta mutant cells, and immunoblotting confirmed that the abundance of their gene products was dramatically reduced. Studies of an overlapping ORE/UAS1 arrangement in the CTA1 promoter revealed synergy between these elements. We conclude that overlapping ORE and UAS1 elements in conjunction with their binding factors Pip2p-Oaf1p and Adr1p coordinate the carbon flux through beta-oxidation with peroxisome proliferation.
authors: Institut für Biochemie und Molekulare Zellbiologie der Universität Wien and Ludwig Boltzmann-Forschungsstelle für Biochemie, Vienna Biocenter, Dr Bohrgasse 9, A-1030 Vienna, Austria. AG@abc.univie.ac.at
authors: A
authors: A
authors: Gurvitz
authors: J K
authors: JK
authors: Hiltunen
authors: R
authors: R
authors: Erdmann
authors: B
authors: B
authors: Hamilton
authors: A
authors: A
authors: Hartig
authors: H
authors: H
authors: Ruis
authors: H
authors: H
authors: Rottensteiner
mesh_terms: D042962:Acyl-CoA Oxidase; D001483:Base Sequence; D017931:DNA Primers; D004268:DNA-Binding Proteins / Q000502:physiology*; D005227:Fatty Acids / Q000378:metabolism*; D005656:Fungal Proteins / Q000502:physiology*; D015966:Gene Expression Regulation, Fungal / Q000502:physiology*; D008565:Membrane Proteins / Q000235:genetics*; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000235:genetics*; D000074401:Peroxins; D020675:Peroxisomes / Q000648:ultrastructure*; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors / Q000502:physiology*
chemical_list: C074682:ADR1 protein, S cerevisiae; D017931:DNA Primers; D004268:DNA-Binding Proteins; D005227:Fatty Acids; D005656:Fungal Proteins; D008565:Membrane Proteins; C092128:PEX11 protein, S cerevisiae; D000074401:Peroxins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D010088:Oxidoreductases; D042962:Acyl-CoA Oxidase; C467991:POX1 protein, S cerevisiae