Influence of a combination of herbs on appetite suppression and weight loss in rats.
OBJECTIVE
We evaluated the ability of a Chinese herbal formulation previously associated with weight loss to influence appetite and weight loss in a carefully controlled laboratory study performed on rats. As a secondary gain, results with this herbal formulation were compared with those from a commonly available phenylpropanolamine (PPA) compound.


METHODS
Eight rats were placed in each arm of a three-arm study, a total of 24 rats. All rats were gavaged with a 2-ml fluid volume containing no addition (control) or the two test substances (combined herbs or PPA) for the first 4 days of the week over 6 consecutive weeks; no gavages were given over weekends. Rats in the two-test groups were given a relatively low dose of the test substance for 3 weeks, followed by a higher dose over the next 3-week periods. Food and water intake were measured for 24-h periods over the ensuing week days. The average daily values for food and water intake for an individual rat were calculated on the basis of collected data over each 3-week period. The mean values for each rat obtained over the low- and high-dose periods comprised results from averaging at least 10 measurements.


RESULTS
Average daily food intake was decreased only with the herbal formulation, not the PPA compound at the low and high doses. Both the PPA compound and the herbal formulation lowered water intake significantly at the low and high doses. Rats ingesting the herbal formulation at the lower dose had statistically significant lower daily body weight changes over the 3 weeks than those ingesting the PPA compound. At the higher dose, body weight changes for both agents were significantly less than the control, but not significantly different from each other. No evidence of toxicity was seen in the blood chemistries or after histopathological examination.


CONCLUSIONS
Data collected on rats suggest that the herbal formulation examined might be a useful and safe combination to overcome the overweight state and obesity.
Talpur
N A
NA
Department of Medicine, Renal Division, Georgetown University Medical Center, Washington, DC 20007, USA.
Echard
B W
BW
Manohar
V
V
Preuss
H G
HG
D000818:Animals; D001067:Appetite Depressants / Q000008:administration & dosage / Q000494:pharmacology*; D001069:Appetite Regulation / Q000187:drug effects*; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology*; D007441:Intubation, Gastrointestinal; D010665:Phenylpropanolamine / Q000008:administration & dosage / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D015431:Weight Loss / Q000187:drug effects*
D001067:Appetite Depressants; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D010665:Phenylpropanolamine