title: Identification of putative proteins involved in granule biogenesis of tick salivary glands.
abstract: Ticks secrete bioactive components during feeding that assist them in gaining a blood meal. Compounds secreted are stored in granules until a stimulus induces secretion during feeding. Biogenesis of tick secretory granules has not been investigated before. An adequate understanding of granule biogenesis could advance our understanding of tick salivary gland biology and could aid in the rational design of tick control methods. Putative tick salivary gland proteins 1-4 (TSGP1-4) involved in granule biogenesis were identified in this study based on their abundance in salivary gland extracts and granule preparations and their ability to aggregate under conditions of slight acidity and high calcium concentration. TSGP2 and TSGP3 have been identified as previously described toxic and nontoxic homologues, respectively, while toxicity was also associated with TSGP4.
authors: Department of Biochemistry, University of Pretoria, South Africa.
authors: B J
authors: BJ
authors: Mans
authors: J D
authors: JD
authors: Venter
authors: P J
authors: PJ
authors: Vrey
authors: A I
authors: AI
authors: Louw
authors: A W
authors: AW
authors: Neitz
mesh_terms: D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional; D019476:Insect Proteins / Q000032:analysis* / Q000378:metabolism; D012469:Salivary Glands / Q000378:metabolism* / Q000648:ultrastructure; D022142:Secretory Vesicles / Q000378:metabolism / Q000648:ultrastructure; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013987:Ticks / Q000378:metabolism*
chemical_list: D019476:Insect Proteins