Participation of type I interferon in the decreased virulence of the UL13 gene-deleted mutant of herpes simplex virus type 1.
We isolated a UL13 gene-deleted mutant of the herpes simplex virus type 1 (HSV-1) strain VR3 (VRDelta13) and its revertant virus (VRDelta13R). This deletion mutant still had virus host shutoff (vhs) activity, although a previous report had suggested the possibility of a functional relation between the UL13 product, that is protein kinase (PK), and vhs activity. We compared the in vivo growth of these viruses in BALB/c mice. VRDelta13 was cleared in the early period of intraperitoneal infection. VRDelta13 had a higher sensitivity to the mouse type I interferon (IFN) and showed a higher level of IFN induction in the study period of infection than did VR3 and VRDelta13R. These results suggest that a nonspecific antiviral response (i.e., the IFN system) may contribute to this rapid inhibition of viral replication in vivo.
Shibaki
T
T
Department of Microbiology and First Department of Surgery, Asahikawa Medical College, Asahikawa, 078-8510, Japan.
Suzutani
T
T
Yoshida
I
I
Ogasawara
M
M
Azuma
M
M
D016475:3T3 Cells; D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D004195:Disease Models, Animal; D004351:Drug Resistance / Q000276:immunology; D005260:Female; D017353:Gene Deletion; D005814:Genes, Viral / Q000235:genetics*; D006561:Herpes Simplex / Q000276:immunology* / Q000401:mortality / Q000821:virology*; D018259:Herpesvirus 1, Human / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity*; D006801:Humans; D007370:Interferon Type I / Q000096:biosynthesis / Q000502:physiology*; D007928:Lethal Dose 50; D051379:Mice; D008807:Mice, Inbred BALB C; D011494:Protein Kinases / Q000235:genetics*; D013379:Substrate Specificity / Q000235:genetics; D014709:Vero Cells; D010948:Viral Plaque Assay; D015678:Viral Structural Proteins / Q000235:genetics*; D014774:Virulence; D014779:Virus Replication / Q000235:genetics
D007370:Interferon Type I; D015678:Viral Structural Proteins; D011494:Protein Kinases; C082380:UL13 protein, Simplexvirus