Microwave treatment of contact lens cases contaminated with acanthamoeba.
OBJECTIVE
Microbially contaminated contact lens cases are a predisposing risk factor for Acanthamoeba keratitis. Several findings have shown that microwave irradiation kills the six Food and Drug Administration test challenge microorganisms. We aimed to determine what effect microwave irradiation has on Acanthamoeba trophozoites and cysts.


METHODS
Different types of contact lens cases were contaminated with trophozoites and cysts of three different Acanthamoeba species (A. comandoni, A. castellanii, A. hatchetti) and were exposed to microwave irradiation for 3, 5, and 8 minutes, respectively.


RESULTS
Trophozoites, as well as cysts of the different Acanthamoeba strains, were effectively killed, even by only 3 minutes of microwave irradiation, and there were no negative effects of irradiation on the contact lens cases themselves.


CONCLUSIONS
We demonstrate that microwave treatment is a very effective, easy, and cheap method to keep contact lens cases free of Acanthamoeba, thus considerably reducing the risk of an Acanthamoeba keratitis.
Hiti
K
K
Department of Ophthalmology, University of Graz, Graz, Austria.
Walochnik
J
J
Faschinger
C
C
Haller-Schober
E M
EM
Aspöck
H
H
D000048:Acanthamoeba / Q000528:radiation effects*; D015823:Acanthamoeba Keratitis / Q000517:prevention & control; D000818:Animals; D003261:Contact Lenses; D004203:Disinfection / Q000379:methods*; D006801:Humans; D008872:Microwaves; D019064:Product Packaging