Visualization of biallelic expression of the imprinted SNRPN gene induced by inhibitors of DNA methylation and histone deacetylation.
We have adapted a fluorescence in situ hybridization (FISH) method to detect nascent RNA molecules of the imprinted SNRPN gene at the initial transcription sites in nuclei of human HL60 and WI38 cells. Simultaneous detection of RNA and DNA of SNRPN by FISH using the cosmid probe confirmed its monoallelic expression in these cell lines. Treatment of both cells by inhibitors of DNA methylation and histone deacetylation resulted in time-dependent increase of the cell population with the biallelic expression of SNRPN.
Kohda
A
A
Laboratory of Molecular and Cellular Biology, Faculty of Bioresources, Mie University, Tsu, Japan.
Taguchi
H
H
Okumura
K
K
D000107:Acetylation; D000483:Alleles; D001324:Autoantigens / Q000235:genetics*; D019175:DNA Methylation; D005786:Gene Expression Regulation / Q000187:drug effects*; D018392:Genomic Imprinting; D018922:HL-60 Cells; D006655:Histone Deacetylases / Q000378:metabolism; D006657:Histones / Q000378:metabolism; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D017411:Ribonucleoproteins, Small Nuclear; D055517:snRNP Core Proteins
D001324:Autoantigens; D006657:Histones; D017411:Ribonucleoproteins, Small Nuclear; C526417:SNRPN protein, human; D055517:snRNP Core Proteins; D006655:Histone Deacetylases