GTG banding pattern on human metaphase chromosomes revealed by high resolution atomic-force microscopy.
Surface topography of human metaphase chromosomes following GTG banding was examined using high resolution atomic force microscopy (AFM). Although using a completely different imaging mechanism, which is based on the mechanical interaction of a probe tip with the chromosome, the observed banding pattern is comparable to results from light microscopy and a karyotype of the AFM imaged metaphase spread can be generated. The AFM imaging process was performed on a normal 2n = 46, XX karyotype and on a 2n = 46, XY, t(2;15)(q23;q15) karyotype as an example of a translocation of chromosomal bands.
Thalhammer
S
S
University München, Institute for Crystallography, Theresienstr. 41, 80333 München, Germany.
Koehler
U
U
Stark
R W
RW
Heckl
W M
WM
D001399:Azure Stains; D002871:Chromosome Banding; D002877:Chromosomes, Human / Q000648:ultrastructure*; D006801:Humans; D018625:Microscopy, Atomic Force; D014357:Trypsin
D001399:Azure Stains; D014357:Trypsin