Improved detection of methicillin-resistant Staphylococcus aureus using phenyl mannitol broth containing aztreonam and ceftizoxime.
We tested a phenyl mannitol broth containing ceftizoxime and aztreonam (PHMB(+)) for detection of methicillin-resistant Staphylococcus aureus (MRSA) with reference MRSA strains and, subsequently, with clinical samples (n = 1,098). All reference MRSA strains induced color change in PHMB(+) after 24 to 72 h of incubation. In a clinical setting, 40 MRSA strains were detected with PHMB(+), compared with only 23 detected with a routine method. Thus, this selective broth significantly (P < 0.001) improved the rate of MRSA detection.
Wertheim
H
H
Infectious Diseases, Erasmus University Medical Center Rotterdam, Rotterdam, The Netherlands. wertheim@bacl.azr.nl
Verbrugh
H A
HA
van Pelt
C
C
de Man
P
P
van Belkum
A
A
Vos
M C
MC
D001398:Aztreonam / Q000494:pharmacology; D001431:Bacteriological Techniques; D015296:Ceftizoxime / Q000494:pharmacology; D002511:Cephalosporins / Q000494:pharmacology; D003470:Culture Media / Q000737:chemistry; D006801:Humans; D008353:Mannitol / Q000378:metabolism*; D016106:Methicillin Resistance; D008997:Monobactams / Q000494:pharmacology; D010637:Phenolsulfonphthalein / Q000378:metabolism*; D013203:Staphylococcal Infections / Q000382:microbiology; D013211:Staphylococcus aureus / Q000145:classification / Q000187:drug effects* / Q000254:growth & development / Q000302:isolation & purification*
D002511:Cephalosporins; D003470:Culture Media; D008997:Monobactams; D008353:Mannitol; D015296:Ceftizoxime; D001398:Aztreonam; D010637:Phenolsulfonphthalein