Reduced sperm counts in guppies (Poecilia reticulata) following exposure to low levels of tributyltin and bisphenol A.
There is increasing evidence that normal male reproductive function can be disrupted by exposure to pollutants in the environment that can exogenously mimic, antagonize or block sex-hormone function. One possible consequence of exposure to these xenobiotics is disruption to spermatogenesis, but results thus far provide only indirect and inconsistent evidence. In this study we exposed adult male guppies (Poeciliidae: Teleostei) to environmentally relevant levels of the common xenobiotics tributyltin (11.2-22.3 ngl-1) and bisphenol A (274-549 micrograms l-1) in experimental aquaria. After 21 days of exposure, we found significant declines (by 40-75%) in total sperm counts for male fishes exposed to tributyltin and bisphenol A compared with controls. This short-term decline in sperm count is unlikely to be the result of endocrine-mediated alteration of the germ line, and we found no change in testis size or sperm lengths between treatments. However, Sertoli cells, which facilitate the transport of maturing sperm into the testicular deferent duct (where they are stored prior to ejaculation), are directly sensitive to xenobiotic action and it is therefore possible that spermatogenesis was inhibited through in vivo interference with normal Sertoli-cell function.
Haubruge
E
E
Unit of General and Applied Zoology, University of Agricultural Sciences, B-5030 Gembloux, Belgium.
Petit
F
F
Gage
M J
MJ
D000818:Animals; D001559:Benzhydryl Compounds; D008297:Male; D010636:Phenols / Q000633:toxicity*; D011036:Poecilia / Q000033:anatomy & histology / Q000502:physiology*; D013076:Sperm Count; D013091:Spermatogenesis / Q000187:drug effects*; D014220:Trialkyltin Compounds / Q000633:toxicity*; D015262:Xenobiotics / Q000633:toxicity; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D010636:Phenols; D014220:Trialkyltin Compounds; D015262:Xenobiotics; C011559:tributyltin; C006780:bisphenol A; D000098826:Bisphenol A Compounds