Evaluation of tissue-factor production by human endothelial cells incubated with three acrylic bone cements.
The effect of three methacrylate-based cements used for the fixation of joint prostheses on tissue-factor production by human umbilical vein endothelial cells was evaluated in vitro. The extracts in the culture medium of the cements were tested after 1-h and 7-day curing. The endothelial cells were incubated with the cement extracts for 4 h, and then the tissue factor was determined in cell lysates with both the recalcification time and enzyme immuno assay. The cements did not induce significant production of tissue factor and, therefore, did not activate the extrinsic pathway of coagulation within the limits of the mechanism considered.
Cenni
E
E
Dipartimento Putti, Istituti Ortopedici Rizzoli, Bologna, Italy. elisabetta.cenni@ior.it
Ciapetti
G
G
Granchi
D
D
Savarino
L
L
Stea
S
S
Corradini
A
A
Di Leo
A
A
D001205:Ascorbic Acid / Q000737:chemistry; D001466:Barium Sulfate / Q000737:chemistry; D001585:Benzoyl Peroxide / Q000737:chemistry; D001843:Bone Cements / Q000737:chemistry / Q000494:pharmacology*; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000187:drug effects / Q000502:physiology*; D000431:Ethanol / Q000737:chemistry; D006801:Humans; D007124:Immunoenzyme Techniques; D007700:Kinetics; D008689:Methacrylates / Q000494:pharmacology*; D019904:Polymethyl Methacrylate / Q000737:chemistry; D013329:Structure-Activity Relationship; D013925:Thromboplastin / Q000096:biosynthesis*; D013997:Time Factors; D014471:Umbilical Veins
D001843:Bone Cements; D008689:Methacrylates; D001466:Barium Sulfate; D000431:Ethanol; D019904:Polymethyl Methacrylate; D013925:Thromboplastin; D001205:Ascorbic Acid; D001585:Benzoyl Peroxide