Detection of immune system cells in paraffin wax-embedded ovine tissues.
This report describes a method (fixation, paraffin wax-embedding and immunolabelling) for the demonstration of several immune system cell epitopes (CD1, CD2, CD4, CD8, CD14, CD21, CD45R, WC-1, 28 kDa surface antigen, immunoglobulins and MHC II antigens) in ovine lymph nodes collected at necropsy. Cell surface epitopes considered to be sensitive to processing methods were successfully demonstrated by a procedure that included the use of a non-aldehyde-containing, zinc salts-based fixative, coupled with a sensitive system of immunolabelling. This novel method had the advantage of avoiding antigen-retrieval steps and of providing consistently good morphological definition.
González
L
L
Moredun Research Institute, Pentlands Science Park, Penicuik, Midlothian, EH26 0PZ, UK.
Anderson
I
I
Deane
D
D
Summers
C
C
Buxton
D
D
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D015703:Antigens, CD / Q000032:analysis*; D000939:Epitopes / Q000032:analysis; D019084:Fluorescent Antibody Technique, Indirect / Q000662:veterinary; D007124:Immunoenzyme Techniques / Q000662:veterinary; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology*; D016612:Paraffin Embedding / Q000662:veterinary*; D012756:Sheep / Q000276:immunology*; D014021:Tissue Preservation / Q000662:veterinary*
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000939:Epitopes