MinK-related peptide 1: A beta subunit for the HCN ion channel subunit family enhances expression and speeds activation.
The HCN family of ion channel subunits underlies the currents I(f) in heart and I(h) and I(q) in the nervous system. In the present study, we demonstrate that minK-related peptide 1 (MiRP1) is a beta subunit for the HCN family. As such, it enhances protein and current expression as well as accelerating the kinetics of activation. Because MiRP1 also functions as a beta subunit for the cardiac delayed rectifier I(Kr), these results suggest that this peptide may have the unique role of regulating both the inward and outward channels that underlie cardiac pacemaker activity. The full text of this article is available at http://www.circresaha.org.
Yu
H
H
Institute of Molecular Cardiology, Department of Physiology & Biophysics, Stony Brook, NY, USA.
Wu
J
J
Potapova
I
I
Wymore
R T
RT
Holmes
B
B
Zuckerman
J
J
Pan
Z
Z
Wang
H
H
Shi
W
W
Robinson
R B
RB
El-Maghrabi
M R
MR
Benjamin
W
W
Dixon
J
J
McKinnon
D
D
Cohen
I S
IS
Wymore
R
R
D000818:Animals; D015152:Blotting, Northern; D002478:Cells, Cultured; D054815:Cyclic Nucleotide-Gated Cation Channels; D015870:Gene Expression; D064428:Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels; D015640:Ion Channel Gating / Q000502:physiology; D007473:Ion Channels / Q000235:genetics / Q000378:metabolism*; D008564:Membrane Potentials / Q000502:physiology; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family; D009124:Muscle Proteins; D009419:Nerve Tissue Proteins; D009865:Oocytes / Q000166:cytology / Q000378:metabolism; D018408:Patch-Clamp Techniques; D015221:Potassium Channels / Q000235:genetics / Q000378:metabolism*; D024642:Potassium Channels, Voltage-Gated; D021122:Protein Subunits; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D011817:Rabbits; D051381:Rats; D014162:Transfection; D014982:Xenopus laevis
D054815:Cyclic Nucleotide-Gated Cation Channels; D064428:Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels; D007473:Ion Channels; D009124:Muscle Proteins; D009419:Nerve Tissue Proteins; D015221:Potassium Channels; D024642:Potassium Channels, Voltage-Gated; D021122:Protein Subunits; D012333:RNA, Messenger; C059983:potassium channel protein I(sk)