Simultaneous amplification of DNA and RNA virus using multiplex PCR system.
A large number of disease-causing bacteria and viruses are being sequenced and PCR is increasingly used for the diagnosis of the diseases. We have designed a multiplex PCR system for hepatitis B virus (HBV), a DNA virus, and hepatitis E virus (HEV), an RNA virus. A modified technique has been standardized for simultaneous extraction of DNA and RNA, followed by a one-step RT-PCR/PCR.
Singh
V K
VK
School of Biotechnology, Devi Ahilya University, Khandwa Road, Indore, 452 017, India.
Naik
S
S
D004247:DNA / Q000097:blood / Q000737:chemistry* / Q000302:isolation & purification; D004267:DNA Viruses / Q000737:chemistry*; D006515:Hepatitis B virus / Q000737:chemistry; D016752:Hepatitis E virus / Q000737:chemistry; D006801:Humans; D021141:Nucleic Acid Amplification Techniques; D016133:Polymerase Chain Reaction / Q000379:methods; D012313:RNA / Q000097:blood / Q000737:chemistry* / Q000302:isolation & purification; D012328:RNA Viruses / Q000737:chemistry*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D012313:RNA; D004247:DNA