title: Accuracy limits in the determination of absolute optical properties using time-resolved NIR spectroscopy.
abstract: We assess typical systematic experimental errors involved in a time-resolved measurement as applied to NIR diffuse optical spectroscopy and investigate their effect on the quantification accuracy of the absorption and the reduced scattering coefficient. We demonstrate that common systematic experimental uncertainties may lead to quantification errors of 10% or more, even when excellent signal to noise ratio conditions exist and accurate photon propagation models are employed. We further demonstrate that the accuracy of the calculation depends nonlinearly on the optical properties of the medium measured. High scattering and low absorbing media can be quantified more accurately than media with low scattering or high absorption using measurements of the same signal to noise ratio. We further discuss curve-shape fitting schemes that aid in improving the quantification accuracy in the presence of experimental errors. Finally, we identify uncertainties that set quantification accuracy limits and we find temporal resolution as the ultimate limiting factor in the quantification accuracy achieved. Our findings suggest that temporal resolution of the order of 10 ps is necessary for quantifying the absorption and reduced scattering coefficient of diffuse media with accuracy better than 5% using curve fitting methods. In that sense this analysis can be used in time-resolved system design and in predicting the expected errors given the technology selected for time-resolved measurements.
authors: Departments of Bioengineering, Biochemistry/Biophysics, University of Pennsylvania, Philadelphia, Pennsylvania 19104-6089, USA.vasilis@helix.mgh.harvard.edu
authors: V
authors: V
authors: Ntziachristos
authors: B
authors: B
authors: Chance
mesh_terms: D055592:Biophysical Phenomena; D001703:Biophysics; D005336:Fiber Optic Technology; D006801:Humans; D008962:Models, Theoretical; D055095:Optics and Photonics; D017785:Photons; D012542:Scattering, Radiation; D012680:Sensitivity and Specificity; D019265:Spectroscopy, Near-Infrared / Q000379:methods / Q000706:statistics & numerical data*