On the isolation of polychlorinated dibenzo-p-dioxins and furans from serum samples using immunoaffinity chromatography prior to high-resolution gas chromatography-mass spectrometry.
Immunoaffinity chromatography (IAC) for the purification of polychlorinated dibenzo-p-dioxins and furans (PCDD/Fs) from biological samples was explored as a means to simplify the cleanup procedure and thereby decrease the time and cost of dioxin analysis. A monoclonal antibody (DD3) was used to produce IAC columns and to isolate the PCDD/Fs from serum. Native and 13C-labeled PCDD/Fs were spiked at the ppq to ppt range into serum. Quantitation of the PCDD/Fs was performed by a standard dioxin analytical method, i.e. high-resolution gas chromatography-mass spectrometry (GC-MS), which was easily compatible with IAC. Five of the most toxic PCDD/Fs consistently showed acceptable recoveries (>25%) and were reliably quantitated. The congeners specifically recovered by this method represent almost 80% of the toxic equivalency of dioxins and furans present in the serum samples. Dioxin-like polychlorinated biphenyls (PCBs) were not recognized by this antibody column. Compared to conventional dioxin cleanup methods, IAC decreased solvent usage by 1.5 l/sample and took only 2 h to process a sample for analysis.
Huwe
J K
JK
USDA, ARS, Biosciences Research Laboratory, Fargo, ND 58105, USA. huwej@fargo.ars.usda.gov
Shelver
W L
WL
Stanker
L
L
Patterson
D G
DG
Turner
W E
WE
D001572:Benzofurans / Q000097:blood / Q000302:isolation & purification*; D002846:Chromatography, Affinity / Q000379:methods*; D000072338:Dibenzofurans, Polychlorinated; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D000072317:Polychlorinated Dibenzodioxins / Q000031:analogs & derivatives* / Q000097:blood / Q000302:isolation & purification*
D001572:Benzofurans; D000072338:Dibenzofurans, Polychlorinated; D000072317:Polychlorinated Dibenzodioxins