title: Reveal 8-Hour Test System for detection of Escherichia coli O157:H7 in raw ground beef, raw beef cubes, and iceberg lettuce rinse: collaborative study.
abstract: Five different food types were analyzed by the Reveal for E. coli O157:H7 8-Hour Test System (Reveal 8) and either the U.S. Food and Drug Administration's Bacteriological Analytical Manual (BAM) culture method or the U.S. Department of Agriculture Food Safety Inspection Service (FSIS) culture method for the presence of E. coli O157:H7. A total of 27 laboratories representing academia and private industry in the United States and Canada participated. Food types were inoculated with E. coli O157:H7 at 2 different levels: a high level where predominantly positive results were expected, and a low level where fractional recovery was anticipated. During this study, 1,110 samples and controls were analyzed by both the Reveal 8 and by BAM or FSIS by each of the collaborators (2,220 samples in total). For each set of samples, 740 were artificially inoculated with E. coli O157:H7, and 370 were uninoculated controls. The Reveal 8 detected 528 presumptive positives of which 487 were confirmed positive by the BAM culture method. In comparison, BAM and FSIS detected 489 of the 740 artificially contaminated samples as positive. In an additional in-house study performed only on chilled and frozen raw ground beef, 240 artificially inoculated samples were analyzed by both the Reveal 8 and by FSIS. The Reveal 8 detected and confirmed 104 samples as positive compared to 79 confirmed positive by FSIS.
authors: Neogen Corp, Lansing, MI 48912, USA.
authors: C B
authors: CB
authors: Bird
authors: R J
authors: RJ
authors: Hoerner
authors: L
authors: L
authors: Restaino
mesh_terms: D000818:Animals; D001628:Beverages / Q000382:microbiology; D002417:Cattle; D019453:Escherichia coli O157 / Q000254:growth & development / Q000302:isolation & purification*; D005516:Food Microbiology / Q000592:standards*; D005615:Freezing; D005638:Fruit; D007753:Laboratories; D018545:Lactuca / Q000382:microbiology*; D008460:Meat / Q000382:microbiology*; D008828:Microbiological Techniques; D014481:United States; D016885:United States Department of Agriculture; D014486:United States Food and Drug Administration