[The cloning and verification of the Sox9 gene of the rice field eel].
The Sox 9 gene plays important roles in sex determination, which is conserved in a variety of species including mammals and chicken. The positive lambda clone of the Sox 9 gene of the rice field eel was analyzed by restriction enzymes and the restriction map was constructed. This clone was further verified to be the Sox9 gene by PCR amplification and sequencing of the HMG box region. The cloning of the Sox9 gene of the rice field eel further suggests the evolutionary conservation of this gene.
Liu
L
L
College of Life Sciences, Wuhan University, Wuhan 430072, China.
Guo
Y Q
YQ
Zhou
R J
RJ
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004524:Eels / Q000235:genetics*; D006609:High Mobility Group Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D055758:SOX9 Transcription Factor; D014157:Transcription Factors / Q000235:genetics*
D006609:High Mobility Group Proteins; D055758:SOX9 Transcription Factor; D014157:Transcription Factors