[Two virus strains of isolated in China are recombinant alphaviruses].
OBJECTIVE
To determine the classification, phylogenetic and genetic type of XJ-90260 and XJ-91006 viruses isolated in China.


METHODS
Two pairs of specific primers were designed to amplify the NSP4 and E1-3' UTR genes of XJ-90260 and XJ-91006 viruses and the nucleotide sequences between them and with that of other Alphavirus were compared.


RESULTS
The nucleotide sequences identity between XJ-90260 and XJ-91006 viruses was 100% and has showed the highest with WEE, comparing with the other 26 Alphaviruses in 3'-UTR. NSP4 gene of XJ-90260 and XJ-91006 viruses high homologous with EEE virus and E-1 gene high homologous wih SIN virus. XJ-90260 and XJ-91006 viruses belong to the B group of WEE and are closest to the Russian strain (Y62-33) by phylogenetic analysis.


CONCLUSIONS
XJ-90260 and XJ-91006 viruses isolated in China are recombinant Alphaviruses, they belong to the same genetic type as WEE does.
He
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H
Institute of Virology, Chinese Academy of Preventive Medicine, Beijing 100052, China.
Lu
X
X
Yang
Y
Y
D000524:Alphavirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D020241:Encephalomyelitis, Western Equine / Q000235:genetics*; D010802:Phylogeny; D012689:Sequence Homology, Nucleic Acid