Release of recombinant human bone morphogenetic protein-2 from heterocyclic methacrylate polymer systems.
The release of recombinant human bone morphogenetic protein-2 (rhBMP-2) from three room temperature polymerising methacrylate systems has been studied. These all contained poly(ethyl methacrylate) powder, but the monomer liquids comprised, respectively, tetrahydrofurfuryl methacrylate (THFM), 90/10 THFM/hydroxyethyl methacrylate (HEMA), and 70/30 THFM/ HEMA. In all cases, rhBMP-2 was released, but the addition of 10% HEMA accelerated release (a nine-fold increase in diffusion coefficient); a further increase to 30% HEMA had no additional effect. For most of the release process, a diffusion process operated, although the early stages were not well defined. At the end of the 15 day period, the release, respectively, for the PEM/THFM, PEM:90/10 THFM/HEMA and PEM:70/30 THFM/HEMA systems was 596, 878 and 923 ng (i.e. up to 92% of the rhBMP-2 added).
Patel
M P
MP
Department of Biomaterials in Relation to Dentistry, St Bartholomews and the Royal London School of Medicine and Dentistry, Queen Mary and Westfield College, UK. m.patel@mds.qmw.ac.uk
Pavlovic
P
P
Hughes
F J
FJ
King
G N
GN
Cruchley
A
A
Braden
M
M
D001672:Biocompatible Materials / Q000737:chemistry; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D008689:Methacrylates / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D011485:Protein Binding; D011994:Recombinant Proteins / Q000378:metabolism*; D013696:Temperature; D013997:Time Factors; D016212:Transforming Growth Factor beta
C525718:BMP2 protein, human; D001672:Biocompatible Materials; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D008689:Methacrylates; D011108:Polymers; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; C499469:recombinant human bone morphogenetic protein-2