[Airborne dust particles in indoor environment and allergy].
BACKGROUND
The overall aim of this study was to investigate how airborne house dust particles may contribute to an allergic immune response, and thereby also to asthma and allergic diseases.


METHODS
Using transmission electron microscopy, we quantified and characterized airborne house dust particles, with regard to elemental and size distribution. Furthermore, an immunogold labelling technique was used to study whether some common allergens were present on the surface of airborne house dust particles. Finally, a mouse model was used to study the adjuvant activity of airborne house dust on the IgE antibody response.


RESULTS
A vast majority of the airborne particles samples from homes in Oslo were found to be less than 2.5 microns in diameter, thus they are liable to penetrate deep into the respiratory tree. This PM2.5 fraction contained, in addition to sulphur aerosols and silicates, many soot particles, most of them being less than 1 micron in diameter. These soot particles were found to carry allergens on their surface. We also found that diesel exhaust particles, which is probably a main soot component of airborne house dust, absorbed several wellknown allergens in vitro. Furthermore, the airborne house dust particles were found to elicit a local lymph node response, and to have an adjuvant activity on the production of IgE antibodies to ovalbumin as a model allergen.


CONCLUSIONS
These results show that indoor suspended particulate matter contains a lot of potential allergen carriers, i.e. soot particles (carbon aggregates), most of them being less that 1 micron in diameter and thereby able to transport allergens deep into the airways. In addition, our results indicate that suspended particulate matter may have an adjuvant effect on the production of IgE to common environmental allergens, and also may provoke a local inflammatory response.
Ormstad
H
H
Avdeling for miljømedisin, Statens institutt for folkehelse, Postboks 4404, Nydalen, 0403 Oslo. heidi.ormstad@folkehelsa.no
D000393:Air Pollutants / Q000009:adverse effects* / Q000032:analysis; D016902:Air Pollution, Indoor / Q000009:adverse effects* / Q000032:analysis; D000485:Allergens / Q000009:adverse effects* / Q000032:analysis; D004391:Dust / Q000009:adverse effects* / Q000032:analysis; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000139:chemically induced / Q000209:etiology / Q000276:immunology; D017348:Microscopy, Electron, Scanning Transmission; D009664:Norway; D010316:Particle Size; D012130:Respiratory Hypersensitivity / Q000139:chemically induced / Q000209:etiology* / Q000276:immunology; D013457:Sulfur Compounds / Q000009:adverse effects / Q000032:analysis
D000393:Air Pollutants; D000485:Allergens; D004391:Dust; D013457:Sulfur Compounds