Heparin column analysis of serum type 5 tartrate-resistant acid phosphatase isoforms.
The objective of the present study was to develop a specific method for the separation of tartrate-resistant acid phosphatase (TRAP) derived exclusively from osteoclasts. Heparin column-bound TRAP in human serum was separated into three peaks of TRAP activity when eluted with a linear gradient of sodium chloride. The last peak corresponded to TRAP 5b which was first named according to its electrophoretic mobility [Clin. Chem. 24 (1978) 309] and was considered to be derived from osteoclasts [J. Bone Miner. Res. 13 (1998) 683]. The second peak was found to be TRAP 5a. The height of the last peak varied from age to age.
Igarashi
Y
Y
Dokkyo University School of Medicine, Department of Biochemistry, Mibu Tochigi, Japan. bioiga@dokkyomed.ac.jp
Lee
M Y
MY
Matsuzaki
S
S
D000135:Acid Phosphatase / Q000032:analysis*; D002853:Chromatography, Liquid / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D006493:Heparin / Q000737:chemistry*; D006801:Humans; D007527:Isoenzymes / Q000032:analysis*; D010010:Osteoclasts / Q000201:enzymology; D013056:Spectrophotometry, Ultraviolet; D000071681:Tartrate-Resistant Acid Phosphatase
D007527:Isoenzymes; D006493:Heparin; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase