title: Structural features of arabinogalactan-proteins from the fruit of Lycium chinense Mill.
abstract: In addition to the other arabinogalactan-proteins (AGPs) (Cp-1-C and -D) already reported, two kinds of AGP (Cp-2-B and Hp-2-C) were obtained from the fruit of Lycium chinense Mill. The ratio of arabinose to galactose was approximately 1:1 in both samples, and the carbohydrate was linked O-glycosidically to serine in Cp-2-B, and to both serine and threonine residues of the protein in Hp-2-C. The weight-average molecular weight was 71,000 for Cp-2-B and 120,000 for Hp-2-C. Both samples also contained non-reducing terminal 3-O- and 4-O-substituted galacturonic acids. The ratio of 6-O-substituted galactose (linear part) and 3,6-di-O-substituted galactose (branching point) was almost unity in both samples, being obviously different from the case of Cp-1-C (predominant in the branching domain) and Cp-1-D (predominant in the linear domain). These results offer fresh insight into the grouping of the AGPs, based on the ratio of 6-O- and 3,6-di-O-substituted galactosyl residues.
authors: The United Graduate School of Agricultural Science, Gifu University, Science of Biological Resources, 1-1 Yanagido, Gifu 501-1193, Japan.
authors: X
authors: X
authors: Qin
authors: R
authors: R
authors: Yamauchi
authors: K
authors: K
authors: Aizawa
authors: T
authors: T
authors: Inakuma
authors: K
authors: K
authors: Kato
mesh_terms: D000596:Amino Acids / Q000032:analysis; D002236:Carbohydrate Conformation; D005638:Fruit / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D008970:Molecular Weight; D009088:Mucoproteins / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry*
chemical_list: D000596:Amino Acids; D009088:Mucoproteins; D010940:Plant Proteins; C006619:arabinogalactan proteins