Dendritic cells recruitment and in vivo priming of CD8+ CTL induced by a single topical or transepithelial immunization via the buccal mucosa with measles virus nucleoprotein.
The buccal mucosa, a prototype of pluristratified mucosal epithelia, contains a network of directly accessible class II(+) epithelial dendritic cells (DC), similar to skin Langerhans cells. We showed that a single buccal immunization with measles virus nucleoprotein (NP), by either topical application onto or intradermal injection in the buccal mucosa, induced in vivo priming of protective class I-restricted specific CD8(+) CTL. Both routes of immunization with NP induced a rapid recruitment of DC into the mucosa, which peaked at 2 h and decreased by 24 h. Treatment of mice with Flt3 ligand resulted in an increased number of DC in the buccal mucosa and enhanced the frequency of IFN-gamma-producing NP-specific effectors and the NP-specific CTL response generated after buccal immunization with NP. Finally, NP-pulsed bone marrow-derived DC induced NP-specific IFN-gamma-producing cells upon adoptive transfer to naive mice. These data demonstrate that a viral protein delivered to DC of the buccal mucosa induces in vivo priming of protective anti-viral CD8(+) CTL.
Etchart
N
N
Institut National de la Santé et de la Recherche Médical, Unité 404, Immunité et Vaccination, Lyon, France.
Desmoulins
P O
PO
Chemin
K
K
Maliszewski
C
C
Dubois
B
B
Wild
F
F
Kaiserlian
D
D
D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000278:Administration, Buccal; D000279:Administration, Cutaneous; D000818:Animals; D017951:Antigen Presentation; D001854:Bone Marrow Cells / Q000276:immunology / Q000821:virology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D002455:Cell Division / Q000276:immunology; D002465:Cell Movement / Q000276:immunology*; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology* / Q000637:transplantation / Q000821:virology; D004216:Distemper / Q000401:mortality / Q000517:prevention & control; D004217:Distemper Virus, Canine / Q000276:immunology; D004306:Dose-Response Relationship, Immunologic; D018984:Epitopes, T-Lymphocyte / Q000276:immunology; D005260:Female; D007271:Injections, Intradermal; D008213:Lymphocyte Activation / Q000276:immunology*; D008458:Measles Vaccine / Q000008:administration & dosage / Q000276:immunology; D008565:Membrane Proteins / Q000008:administration & dosage; D051379:Mice; D008807:Mice, Inbred BALB C; D009061:Mouth Mucosa / Q000166:cytology / Q000276:immunology* / Q000821:virology; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology* / Q000821:virology; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000276:immunology; D014764:Viral Proteins / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology
D000276:Adjuvants, Immunologic; D018984:Epitopes, T-Lymphocyte; D008458:Measles Vaccine; D008565:Membrane Proteins; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins; D014614:Vaccines, Synthetic; D014764:Viral Proteins; C084881:flt3 ligand protein; C058314:nucleoprotein, Measles virus