Spontaneous erythromycin resistance mutation in a 23S rRNA gene, rrlA, of the extreme thermophile Thermus thermophilus IB-21.
Spontaneous, erythromycin-resistant mutants of Thermus thermophilus IB-21 were isolated and found to carry the mutation A2058G in one of two 23S rRNA operons. The heterozygosity of these mutants indicates that A2058G confers a dominant or codominant phenotype in this organism. This mutation provides a valuable tool for the genetic manipulation of the 23S rRNA genes of Thermus.
Gregory
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J. W. Wilson Laboratory, Department of Molecular and Cell Biology and Biochemistry, Brown University, Providence, Rhode Island 02912, USA. Steven_Gregory@Brown.edu
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Dahlberg
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D001483:Base Sequence; D004352:Drug Resistance, Microbial / Q000235:genetics; D018432:Drug Resistance, Multiple; D004917:Erythromycin / Q000494:pharmacology*; D005798:Genes, Bacterial; D008034:Lincomycin / Q000494:pharmacology; D008969:Molecular Sequence Data; D009154:Mutation; D009690:Nucleic Acid Conformation; D012329:RNA, Bacterial / Q000737:chemistry / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000737:chemistry / Q000235:genetics*; D016963:Thermus thermophilus / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification
D012329:RNA, Bacterial; D012338:RNA, Ribosomal, 23S; D004917:Erythromycin; D008034:Lincomycin