Modular structure, local flexibility and cold-activity of a novel chitobiase from a psychrophilic Antarctic bacterium.
The gene archb encoding for the cell-bound chitobiase from the Antarctic Gram-positive bacterium Arthrobacter sp. TAD20 was cloned and expressed in Escherichia coli in a soluble form. The mature chitobiase ArChb possesses four functionally independent domains: a catalytic domain stabilized by Ca(2+), a galactose-binding domain and an immunoglobulin-like domain followed by a cell-wall anchorage signal, typical of cell-surface proteins from Gram-positive bacteria. Binding of saccharides was analyzed by differential scanning calorimetry, allowing to distinguish unequivocally the catalytic domain from the galactose-binding domain and to study binding specificities. The results suggest that ArChb could play a role in bacterium attachment to natural hosts. Kinetic parameters of ArChb demonstrate perfect adaptation to catalysis at low temperatures, as shown by a low activation energy associated with unusually low K(m) and high k(cat) values. Thermodependence of these parameters indicates that discrete amino acid substitutions in the catalytic center have optimized the thermodynamic properties of weak interactions involved in substrate binding at low temperatures. Microcalorimetry also reveals that heat-lability, a general trait of psychrophilic enzymes, only affects the active site domain of ArChb.
Lonhienne
T
T
Laboratory of Biochemistry, Institute of Chemistry B6, University of Liege, Liege, B-4000, Belgium.
Zoidakis
J
J
Vorgias
C E
CE
Feller
G
G
Gerday
C
C
Bouriotis
V
V
D000118:Acetylglucosaminidase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D000222:Adaptation, Physiological; D000864:Antarctic Regions; D001173:Arthrobacter / Q000201:enzymology* / Q000235:genetics; D001665:Binding Sites; D002118:Calcium / Q000378:metabolism; D002152:Calorimetry, Differential Scanning; D020134:Catalytic Domain; D003080:Cold Temperature; D004789:Enzyme Activation; D005690:Galactose / Q000378:metabolism; D007700:Kinetics; D018583:Pliability; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D013816:Thermodynamics
D011994:Recombinant Proteins; D000118:Acetylglucosaminidase; D002118:Calcium; D005690:Galactose