Time-dependent expression and processing of a hypothetical protein of possible importance for regulation of the Chlamydia pneumoniae developmental cycle.
Chlamydia pneumoniae is an obligate intracellular human pathogen infecting epithelial cells of the upper respiratory tract. It is a Gram-negative bacteria and has a unique biphasic developmental cycle. In this study, we use two-dimensional gel electrophoresis in combination with radioactive labeling to investigate time-dependent expression and processing of C. pneumoniae proteins. We report on (i) the identification of a hypothetical protein which is expressed late in the developmental cycle and subsequently processed; we speculate that this protein may be of importance for the developmental cycle of Chlamydia; (ii) the identification of the major outer membrane protein in three different variants, which may all be present in vivo.
Vandahl
B B
BB
Department of Medical Microbiology and Immunology, University of Aarhus, Denmark. vandahl@medmicro.au.dk
Gevaert
K
K
Demol
H
H
Hoorelbeke
B
B
Holm
A
A
Vandekerckhove
J
J
Christiansen
G
G
Birkelund
S
S
D001426:Bacterial Proteins / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D016993:Chlamydophila pneumoniae / Q000502:physiology*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
D001426:Bacterial Proteins