title: [Enzymatic conversion of cephalosporin C to glutaryl-7-aminocephalosporanic acid using whole cells of the yeast Trigonopsis variabilis FA10].
abstract: A process for the production of glutaryl-7-aminocephalosporanic acid (GL-7ACA) from cephalosporin C(CPC) using permeabilized cells of yeast Trigonopsis variabilis FA10 containing D-amino acid oxidase (DAO) is described. It was found that the bioconversion of CPC to GL-7ACA was interfered by the catalase activity presented in the cells that hydrolyzed the hydrogen peroxide and resulted in the accumulation of alpha-keto-adipyl-7-ACA (AKA-7ACA) and decrease of GL-7ACA yield. the methods to overcome this problem including the addition of extra H2O2 and use of catalase inhibitor, NaN3, were developed and the rate of GL-7ACA from CPC were 73% and 70.1%, respectively. Another alternative method was to incubate the permeabilized FA10 cells at pH10.5-11.0 for 30 minutes at 20 degrees C which served to selectively inactivate the catalase. In the bioconversion of CPC to GL-7ACA using pH10.5-treated cells without catalase activity, the high reaction yield of GL-7ACA(84%) was achieved.
authors: Shanghai Institute of Plant Physiology, Chinese Academy of Sciences, Shanghai 200032, China.
authors: J
authors: J
authors: Chen
authors: T B
authors: TB
authors: Zhu
authors: Y F
authors: YF
authors: Zhang
authors: Y L
authors: YL
authors: Yang
authors: R S
authors: RS
authors: Jiao
mesh_terms: D002511:Cephalosporins / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D019810:Sodium Azide / Q000494:pharmacology; D015003:Yeasts / Q000254:growth & development / Q000378:metabolism*
chemical_list: D002511:Cephalosporins; C081063:glutaryl-7-aminocephalosporanic acid; C025163:cephalosporin C; D019810:Sodium Azide; D006861:Hydrogen Peroxide