[The effect of EB virus bhrfl gene expressing on the topoisomerase I expression in nasopharyngeal carcinoma cell line].
OBJECTIVE
To study the effect of bhrfl expressing on the topoisomerase I expression in nasopharyngeal carcinoma cell line CNE2.


METHODS
bhrfl recombinant expressing vector was constructed and used to transfect the nasopharyngeal carcinoma cell line CNE2. The CNE2 cells were then tested for changes of the topoisomerase I expression and anti-apoptotic ability under the treatment of camptothecin.


RESULTS
bhrfl expressing could enhance the topoisomerase I expression in CNE2 cells, that in end reduced cell sensitivity to camptothecin increased cell survival span and decreased apoptotic rate(P < 0.01).


CONCLUSIONS
By increasing the expression of topoisomerase I, bhrfl expressing can enhance the resistance of CNE2 cells to apoptosis induced by camptothecin.
Huang
H
H
Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
Pan
X
X
Zhou
J
J
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D002166:Camptothecin / Q000494:pharmacology; D004264:DNA Topoisomerases, Type I / Q000096:biosynthesis* / Q000187:drug effects; D015870:Gene Expression; D005814:Genes, Viral; D004854:Herpesvirus 4, Human / Q000235:genetics*; D006801:Humans; D009303:Nasopharyngeal Neoplasms / Q000201:enzymology* / Q000473:pathology / Q000821:virology; D014407:Tumor Cells, Cultured; D014764:Viral Proteins / Q000096:biosynthesis*
D000972:Antineoplastic Agents, Phytogenic; C083278:BHRF1 protein, Human herpesvirus 4; D014764:Viral Proteins; D004264:DNA Topoisomerases, Type I; D002166:Camptothecin