Changes in zinc levels and superoxide dismutase activities in the skin of acute, ultraviolet-B-irradiated mice after treatment with ginkgo biloba extract.
Acute ultraviolet-B (UV-B) irradiation is known to act as an initiator in the formation of reactive oxygen species. These oxygen products are highly reactive and they are able to cause irreversible damage to cellular components. Oxygen free radicals are normally neutralized by very efficient systems in the body. These include antioxidant enzymes like superoxide dismutase (SOD). In a healthy subject, there is a balance between free radicals and the levels of antioxidants. In some pathological conditions such as oxidative stress, the level of antioxidants is significantly reduced. The skin contains relatively high levels of zinc (Zn), an essential element known to be a cofactor in some metabolic pathways. Zinc has also been reported to have antioxidant properties. In the present study, we investigated the effect of ginkgo biloba extract (Gbe), a potent free-radical scavenger, on UV-B-irradiated skin by measuring SOD activity and Zn levels in the skin, before and after treatment. The SOD activity was decreased after UV-B exposure, in comparison with the control group (p < 0.05). After Gbe treatment, the SOD activity increased (p < 0.05) as compared with the untreated UV-B irradiated group. The Zn levels changed in the same pattern as the SOD activity values.
Aricioglu
A
A
Department of Biochemistry, Medical Faculty, Gazi University, Besevler, Ankara, Turkey.
Bozkurt
M
M
Balabanli
B
B
Kilinç
M
M
Nazaroglu
N K
NK
Türközkan
N
N
D000818:Animals; D005260:Female; D005419:Flavonoids / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000494:pharmacology*; D020441:Ginkgo biloba; D051379:Mice; D008807:Mice, Inbred BALB C; D010936:Plant Extracts / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism; D012867:Skin / Q000187:drug effects* / Q000378:metabolism* / Q000528:radiation effects; D013482:Superoxide Dismutase / Q000378:metabolism*; D014466:Ultraviolet Rays / Q000009:adverse effects; D015032:Zinc / Q000378:metabolism*
D005419:Flavonoids; D016166:Free Radical Scavengers; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D013482:Superoxide Dismutase; D015032:Zinc