title: Increasing the intra-Golgi pH of cultured LS174T goblet-differentiated cells mimics the decreased mucin sulfation and increased Thomsen-Friedenreich antigen (Gal beta1-3GalNac alpha-) expression seen in colon cancer.
abstract: Mucins in ulcerative colitis and colon cancer share common properties of reduced sulfation and increased oncofetal carbohydrate antigen expression. It has previously been shown that there is no simple correlation between these changes and the activity of the relevant glycosyl-, sialyl-, and sulfo-transferases. We examined mucin sulfation and expression of oncofetal Thomsen-Friedenreich (TF) antigen (galactosyl beta1-3N-acetylgalactosamine alpha-) in the goblet cell-differentiated human colon cancer cell line LS174T following treatment with bafilomycin A(1, )which raises intra-Golgi pH, or monensin, which disrupts medial-trans Golgi transport. Cells were dual-labeled with sodium [(35)S]-sulfate and D-[6-(3)H(N)]-glucosamine hydrochloride, or labeled with L-[U-(14)C]-threonine alone. Mucin was purified using Sepharose CL-4B gel filtration. Mucin sulfo-Lewis(a) and TF antigen expression were assessed using the F2 anti-sulfo-Lewis(a) monoclonal antibody and peanut agglutinin binding respectively. Bafilomycin (0.01 microM; 48 h) reduced total mucin sulfation, expressed relative to incorporation of glucosamine, to 0.50 +/- 0.04 d.p.m. [(35)S]-sulfate per d.p.m. [(3)H]-glucosamine compared to control, 0.84 +/- 0.05 (p < 0.001, n = 16). This was accompanied by 50.3 +/- 8.0% increased expression of TF antigen (p < 0.01) and 50.1 +/- 5.5% decreased expression of sulfo-Lewis(a) (p < 0.01). The reduced sulfate:glucosamine ratio was largely due to increased incorporation of glucosamine into newly synthesized mucin rather than reduction in total sulfate incorporation. In contrast, monensin only reduced total mucin glycosylation at concentrations > 0.1 microM and had no significant effect on mucin sulfation or TF expression. Intra-Golgi alkalinization affects mucin glycosylation, resulting in decreased mucin sulfation and increased expression of TF antigen, changes that mimic those seen in cancerous and premalignant human colonic epithelium.
authors: Glycobiology Group, Gastroenterology Research Unit, Department of Medicine, University of Liverpool, Daulby Street, Liverpool, L69 3GA, UK.
authors: B J
authors: BJ
authors: Campbell
authors: G E
authors: GE
authors: Rowe
authors: K
authors: K
authors: Leiper
authors: J M
authors: JM
authors: Rhodes
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000378:metabolism*; D002240:Carbohydrate Sequence; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D003110:Colonic Neoplasms / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D004273:DNA, Neoplasm / Q000378:metabolism; D005944:Glucosamine / Q000378:metabolism; D006031:Glycosylation / Q000187:drug effects; D020397:Goblet Cells / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D006056:Golgi Apparatus / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007983:Lewis Blood Group Antigens; D018942:Macrolides; D008969:Molecular Sequence Data; D008985:Monensin / Q000494:pharmacology; D009077:Mucins / Q000737:chemistry / Q000378:metabolism*; D009844:Oligosaccharides / Q000378:metabolism; D013431:Sulfates / Q000378:metabolism; D014407:Tumor Cells, Cultured
chemical_list: D000900:Anti-Bacterial Agents; D015295:Antigens, Tumor-Associated, Carbohydrate; D004273:DNA, Neoplasm; D007983:Lewis Blood Group Antigens; D018942:Macrolides; D009077:Mucins; D009844:Oligosaccharides; D013431:Sulfates; C116504:sulfo-Lewis(a); C016467:Thomsen-Friedenreich antigen; C040929:bafilomycin A1; D008985:Monensin; D005944:Glucosamine