Serum lipid levels and M/L55 allele distribution of HDL paraoxonase gene in Saami and Finnish men.
Paraoxonase (PON) is an antioxidative enzyme, which eliminates lipid peroxides. The mutation in codon 55 of PON1 gene causes a change of methionine (M-allele) to leucine (L-allele) and influences PON activity. The Saami are a population living in the northern part of Fennoscandia. In previous studies their death rate from coronary artery disease (CAD) was found to be low. We compared PON M/L55 allele frequencies of 68 Saami and 68 Finnish men and related the PON genotypes to plasma lipid levels and to the levels of autoantibodies against oxidized LDL. The M/L55 genotypes were determined by PCR and restriction enzyme digestion. ELISA was used to measure antibodies against oxidized LDL. The L- and M-allele frequencies were 64% and 36% in Saami population and 64% and 36% in Finnish men, respectively (p = NS, Fisher's exact test). There were also no significant differences in plasma lipid levels or in antibody levels against oxidized LDL between PON genotypes or between Saami and Finnish men. Our results indicate that the PON M/L55 genotype is not associated with plasma lipid levels or the levels of autoantibodies against oxidized LDL in these populations. The Saami men have the same PON M/L55 allele distribution as the Finnish men and the PON genotype might thus not be one factor protecting Saami against CAD.
Malin
R
R
University of Tampere, Medical School, Department of Medical Biochemistry, Tampere, Finland. riikka.malin@csc.fi
Lehtinen
S
S
Luoma
P
P
Näyhä
S
S
Hassi
J
J
Koivula
T
T
Lehtimäki
T
T
D000704:Analysis of Variance; D043303:Aryldialkylphosphatase; D002784:Cholesterol / Q000097:blood*; D004950:Esterases / Q000235:genetics* / Q000378:metabolism*; D005006:Ethnicity / Q000235:genetics*; D005387:Finland; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D014280:Triglycerides / Q000097:blood*
D014280:Triglycerides; D002784:Cholesterol; D004950:Esterases; D043303:Aryldialkylphosphatase; C468406:PON1 protein, human