Gene families from the Arabidopsis thaliana pollen coat proteome.
The pollen extracellular matrix contains proteins mediating species specificity and components needed for efficient pollination. We identified all proteins >10 kilodaltons in the Arabidopsis pollen coating and showed that most of the corresponding genes reside in two genomic clusters. One cluster encodes six lipases, whereas the other contains six lipid-binding oleosin genes, including GRP17, a gene that promotes efficient pollination. Individual oleosins exhibit extensive divergence between ecotypes, but the entire cluster remains intact. Analysis of the syntenic region in Brassica oleracea revealed even greater divergence, but a similar clustering of the genes. Such allelic flexibility may promote speciation in plants.
Mayfield
J A
JA
Department of Molecular Genetics and Cell Biology, Howard Hughes Medical Institute, The University of Chicago, Chicago, IL 60637, USA.
Fiebig
A
A
Johnstone
S E
SE
Preuss
D
D
D000483:Alleles; D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000737:chemistry / Q000235:genetics*; D029681:Arabidopsis Proteins; D001937:Brassica / Q000737:chemistry / Q000235:genetics; D020224:Expressed Sequence Tags; D017343:Genes, Plant; D014644:Genetic Variation; D018745:Genome, Plant; D008049:Lipase / Q000737:chemistry* / Q000235:genetics; D008969:Molecular Sequence Data; D005810:Multigene Family; D010770:Phosphotransferases / Q000737:chemistry / Q000235:genetics; D010940:Plant Proteins / Q000737:chemistry* / Q000235:genetics; D011058:Pollen / Q000737:chemistry*; D017434:Protein Structure, Tertiary; D020543:Proteome; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment
D029681:Arabidopsis Proteins; D010940:Plant Proteins; D020543:Proteome; C079353:oleosin protein, Arabidopsis; D010770:Phosphotransferases; D008049:Lipase