title: Enhanced low shear stress induced platelet aggregation by Shiga-like toxin 1 purified from Escherichia coli O157.
abstract: The effect of Shiga-like toxin 1 (Stx1) produced by Escherichia coli O157 on platelets was studied with an argon laser light-assisted shear-induced platelet aggregometer and with binding assays. Stx1 markedly enhanced the platelet aggregation under low shear stress but did not affect it under high shear stress. Minimal concentration of Stx1 required for the enhancement was 0.25 ng/ml, and almost maximal enhancement was observed at a final concentration of > or =2.5 ng/ml. This enhanced platelet aggregation disappeared after leukocyte depletion from normal platelet-rich plasma with a specific filter. In contrast, a standard platelet aggregometer was unable to detect this enhanced platelet aggregation in either the presence or the absence of ADP. 125I-labeled purified Stx1 did not specifically bind to normal washed platelets depleted of leukocytes, and thin-layer chromatographic analysis of glycolipids extracted from normal platelet lysates also confirmed that leukocyte-depleted normal platelets lack Stx1-specific receptor globotriaosylceramide (Gb3). Supernatant from the monocyte suspension stimulated with Stx1 exhibited the enhanced low shear stress induced platelet aggregation, but that from the polymorphonuclear cell suspension did not. Several cytokines produced from monocytes reproduced this event in vitro. Further, plasmas from six out of seven patients with hemolytic uremic syndrome (HUS) had activity similar to the purified Stx1. This activity was almost totally impaired after treatment of HUS plasmas with Gb3 in accord with reduction of plasma Stx1 levels. Taken together, these results indicate that platelets lack Gb3, and Stx1 appears to modulate platelet aggregation in an indirect fashion, presumably by the release of cytokines or chemical compounds from the target tissues.
authors: Department of Second Internal Medicine, Nara Medical University, Kashihara, Japan.
authors: H
authors: H
authors: Yagi
authors: N
authors: N
authors: Narita
authors: M
authors: M
authors: Matsumoto
authors: Y
authors: Y
authors: Sakurai
authors: H
authors: H
authors: Ikari
authors: A
authors: A
authors: Yoshioka
authors: E
authors: E
authors: Kita
authors: Y
authors: Y
authors: Ikeda
authors: K
authors: K
authors: Titani
authors: Y
authors: Y
authors: Fujimura
mesh_terms: D001427:Bacterial Toxins / Q000494:pharmacology; D002648:Child; D002675:Child, Preschool; D016207:Cytokines / Q000378:metabolism / Q000494:pharmacology; D019453:Escherichia coli O157 / Q000737:chemistry*; D005260:Female; D006463:Hemolytic-Uremic Syndrome / Q000097:blood / Q000382:microbiology; D006801:Humans; D007834:Lasers; D007962:Leukocytes / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009391:Nephelometry and Turbidimetry; D010974:Platelet Aggregation / Q000187:drug effects*; D011869:Radioligand Assay; D022622:Shiga Toxin 1 / Q000302:isolation & purification / Q000494:pharmacology*; D013314:Stress, Mechanical; D014281:Trihexosylceramides / Q000494:pharmacology
chemical_list: D001427:Bacterial Toxins; D016207:Cytokines; D022622:Shiga Toxin 1; D014281:Trihexosylceramides; C018549:globotriaosylceramide