Preparation of extended metaphase chromosomes from human cultured cells using a topoisomerase II inhibitor, ICRF-193.
Effects of ICRF-193, a topoisomerase II inhibitor, on metaphase chromosome preparations were examined. A short-time exposure of this drug to human HL60 cells in a suspension culture before harvest resulted in obtaining more extended metaphase chromosomes. The length of chromosome 6 identified by fluorescence in situ hybridization was twice as long with this drug treatment. Together with effectiveness for adherent HepG2 cells, these results suggest that treatments with ICRF-193 provide a simple and reliable method for extended metaphase chromosome preparations from cultured cells.
Kohda
A
A
Laboratory of Molecular and Cellular Biology, Faculty of Bioresources, Mie University, Tsu, Japan.
Taguchi
H
H
Okumura
K
K
D002877:Chromosomes, Human; D054659:Diketopiperazines; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008677:Metaphase; D010879:Piperazines / Q000494:pharmacology*; D059005:Topoisomerase II Inhibitors; D014407:Tumor Cells, Cultured
D054659:Diketopiperazines; D004791:Enzyme Inhibitors; D010879:Piperazines; D059005:Topoisomerase II Inhibitors; C070899:4,4'-(1,2-dimethyl-1,2-ethanediyl)bis-2,6-piperazinedione