title: Effect of endotoxin-induced reactive oxygen species on sperm motility.
abstract: OBJECTIVE
  To define the mechanism of infection-induced damage of sperm.
  
  
  METHODS
  The effect of lipopolysaccharide (LPS)-induced reactive oxygen species (ROS) on sperm motility and its modification by scavengers were investigated.
  
  
  METHODS
  Research laboratory of a university hospital.
  
  
  METHODS
  Normozoospermic semen samples were obtained from 37 healthy volunteers.
  
  
  METHODS
  The sperms were incubated in the presence of LPS with or without scavengers.
  
  
  METHODS
  Sperm motility was evaluated by a sperm quality analyzer (SQAIIB). ROS formation in semen samples was measured by a Berthold luminometer (LB953).
  
  
  RESULTS
  Motility of spermatozoa was decreased in the LPS-treated samples compared with that in the control groups. ROS was significantly higher in the LPS-treated groups than in the control groups. The addition of ROS scavengers restored the motility index and suppressed ROS production in the LPS-treated semen samples.
  
  
  CONCLUSIONS
  These data suggest that endotoxin-induced excessive production of ROS is responsible for the decrease in sperm motility and that antioxidant therapy may be a therapeutic option for infertile men with bacterial genital tract infection.
authors: Department of Obstetrics and Gynecology, Oita Medical University, Hasama, Japan.
authors: K
authors: K
authors: Urata
authors: H
authors: H
authors: Narahara
authors: Y
authors: Y
authors: Tanaka
authors: T
authors: T
authors: Egashira
authors: F
authors: F
authors: Takayama
authors: I
authors: I
authors: Miyakawa
mesh_terms: D004731:Endotoxins / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000494:pharmacology; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013078:Sperm Immobilizing Agents / Q000378:metabolism*; D013081:Sperm Motility / Q000187:drug effects* / Q000502:physiology*; D013094:Spermatozoa / Q000187:drug effects / Q000502:physiology
chemical_list: D004731:Endotoxins; D016166:Free Radical Scavengers; D008070:Lipopolysaccharides; D017382:Reactive Oxygen Species; D013078:Sperm Immobilizing Agents