Exo-arabinanase of Penicillium chrysogenum able to release arabinobiose from alpha-1,5-L-arabinan.
An exo-arabinanase, designated Abnx, was purified from a culture filtrate of Penicillium chrysogenum 31B by ammonium sulfate precipitation, anion-exchange chromatography, and hydrophobic chromatography. Abnx had an apparent molecular mass of 47 kDa. The enzyme released only arabinobiose from the nonreducing terminus of alpha-1,5-L-arabinan and showed no activity towards p-nitrophenyl-alpha-L-arabinofuranoside and alpha-1,5-L-arabinofuranobiose. Abnx is the first enzyme with this mode of action.
Sakamoto
T
T
Division of Applied Biochemistry, Graduate School of Agriculture and Biological Science, Osaka Prefecture University, Osaka 599-8531, Japan. sakamoto@nantes.inra.fr
Thibault
J F
JF
D003470:Culture Media; D004187:Disaccharides / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D010408:Penicillium chrysogenum / Q000201:enzymology* / Q000254:growth & development; D011134:Polysaccharides / Q000378:metabolism*; D013379:Substrate Specificity; D013696:Temperature
D003470:Culture Media; D004187:Disaccharides; D011134:Polysaccharides; C434937:arabinobiose; C030080:araban; D006026:Glycoside Hydrolases; C434938:exo-alpha-L-arabinanase