[Troponine T as possible myocardial injury marker. Its application in myocardial stunning and silent ischemia].
OBJECTIVE
The need for more specific, more sensitive and earlier biochemical markers of acute myocardial infarction, has led to the development of alternative methods to CK-MB). The aim of this work is to assess the usefulness of TnT measurement, in comparison with other markers for detecting transitory ischemic processes without necrosis in some experimental models.


METHODS
The plasma levels of Troponine T, CK, CK-MB and adenosine were assessed as markers of ischemic myocardial injury. Two protocols were used: in Series I and II very brief (2 min ischemia with 3-min reperfusion) repeated (20 episodes) ischemias were induced, while Series III involved a single 15-min ischemia with a 60-min reperfusion. In Series I the coronary occlusor was placed close to the anterior descending coronary artery (AD); in Series II and III it was placed distally in the AD. Blood samples were taken from the peripheral vein (PVB) and corresponding coronary segment vein; in a basal situation, during ischemia, upon reperfusion, after 24 hours, and after 5 and 10 days. The plasma levels of adenosine, troponine T, CK and CK-MB as well as general and regional function parameters were measured.


RESULTS
In Series I we observed hypokinesis that lasted 10 days, reaching its maximum on days 4-5. In Series II and III regional function was restored by 24 hours. CK and CK-MB showed similar behaviour; they rose significantly when the chest was opened (p < 0.05) reaching the highest value at 24 hours in all the series. Adenosine rose significantly only during reperfusion (p < 0.05). Troponine T increased after ischemia but not before, remained high for 5 days in all series (PVB).


CONCLUSIONS
Troponine T rises in absence of necrosis, preferably when the ischemia is longer.
Capdevila
C
C
Centro de Investigación del Hospital Universitario La Fe. Unidades de Investigación Cardiovascular y Biología y Patología Celular. Valencia.
Portolés
M
M
Hernándiz
A
A
Pallarés
V
V
Cosín
J
J
D000241:Adenosine / Q000097:blood; D000818:Animals; D015415:Biomarkers; D003402:Creatine Kinase / Q000097:blood; D004285:Dogs; D017682:Myocardial Stunning / Q000175:diagnosis*; D020107:Troponin T / Q000097:blood*
D015415:Biomarkers; D020107:Troponin T; D003402:Creatine Kinase; D000241:Adenosine