Effects of oxidative stress on the erythrocyte Na+,K+ ATPase activity in female hyperthyroid patients.
This study was planned to determine the effects of free-radical-induced damage on the Na+,K+-ATPase activity of erythrocytes during hyperthyroidism and 4 wk after propylthiouracil (PTU) therapy (400 mg/d). The levels of plasma thiobarbituric acid-reactive substances (TBARS) as a marker of lipid peroxidation, erythrocyte glutathione (GSH) concentration as an antioxidant, blood ATP concentration, and erythrocyte membrane Na+,K+-ATPase activity were determined in female hyperthyroid patients (n = 22, mean age 40.5 +/- 6.5 yr). Before the PTU therapy, plasma TBARS concentration was significantly higher and the levels of blood ATP and erythrocyte GSH and the activity of membrane Na+,K-+-ATPase were significantly lower in the hyperthyroid patients (n = 15 women, mean age 40.8 +/- 7.3 yr). Four weeks after PTU therapy, plasma TBARS concentration was decreased, and levels of erythrocyte GSH and blood ATP and of Na+,K+-ATPase activity of erythrocytes were elevated in the treated patients. There was a significant positive correlation between blood ATP concentration and Na+,K+-ATPase activity, and a negative correlation between plasma TBARS concentration and Na+,K+-ATPase activity before PTU. Our results might help to clarify the effects of the oxidative mechanisms on the erythrocyte membrane Na+,K+-ATPase activity in hyperthyroid patients.
Konukoglu
D
D
Department of Biochemistry, Cerrahpasa Medical Faculty, Istanbul University, Turkey. dkonuk@yahoo.com
Yelke
H K
HK
Hatemi
H
H
Sabuncu
T
T
D000255:Adenosine Triphosphate / Q000097:blood; D000328:Adult; D013956:Antithyroid Agents / Q000494:pharmacology; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000201:enzymology; D004912:Erythrocytes / Q000201:enzymology*; D005260:Female; D006801:Humans; D006980:Hyperthyroidism / Q000201:enzymology*; D018384:Oxidative Stress / Q000502:physiology*; D011441:Propylthiouracil / Q000494:pharmacology; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D013956:Antithyroid Agents; D017392:Thiobarbituric Acid Reactive Substances; D011441:Propylthiouracil; D000255:Adenosine Triphosphate; D000254:Sodium-Potassium-Exchanging ATPase