The tumor suppressor candidate p33(ING1) mediates repair of UV-damaged DNA.
The biological functions of the tumor suppressor, ING1, have been studied extensively in the last 5 years since it was cloned. It shares many biological functions with those of p53 and has been reported to mediate growth arrest, senescence, apoptosis, anchorage-dependent growth, and chemosensitivity. Some of these functions, such as cell cycle arrest and apoptosis, have been shown to be dependent on the activity of both ING1 and p53 proteins. In this study, we report that p33(ING1) (one of ING1 isoforms) is also involved in the modulation of DNA repair. We found that overexpression of p33(ING1) enhances repair of UV-damaged DNA and that p53 is required for the repair process. Furthermore, binding between ING1 and GADD45 has been detected. These observations suggest that p33(ING1) cooperates with p53 in nucleotide excision repair and that GADD45 may be one of its components.
Cheung
K J
KJ
Division of Dermatology, Department of Medicine, University of British Columbia, and Vancouver Hospital and Health Sciences Center, Vancouver, British Columbia V6H 3Z6, Canada.
Mitchell
D
D
Lin
P
P
Li
G
G
D018797:Cell Cycle Proteins; D004249:DNA Damage; D004260:DNA Repair / Q000235:genetics / Q000502:physiology*; D004268:DNA-Binding Proteins; D004307:Dose-Response Relationship, Radiation; D015870:Gene Expression / Q000528:radiation effects; D016147:Genes, Tumor Suppressor; D006367:HeLa Cells; D006801:Humans; D000076023:Inhibitor of Growth Protein 1; D047908:Intracellular Signaling Peptides and Proteins; D008545:Melanoma / Q000235:genetics / Q000378:metabolism; D009687:Nuclear Proteins; D011506:Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D015398:Signal Transduction / Q000502:physiology; D014162:Transfection; D016159:Tumor Suppressor Protein p53 / Q000502:physiology; D025521:Tumor Suppressor Proteins; D014466:Ultraviolet Rays; D000097642:GADD45 Proteins
D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D000076023:Inhibitor of Growth Protein 1; D047908:Intracellular Signaling Peptides and Proteins; D009687:Nuclear Proteins; D011506:Proteins; D016159:Tumor Suppressor Protein p53; D025521:Tumor Suppressor Proteins; D000097642:GADD45 Proteins; C501503:ING1 protein, human