Lentinula edodes produces a multicomponent protein complex containing manganese (II)-dependent peroxidase, laccase and beta-glucosidase.
A multicomponent protein complex containing manganese (II)-dependent peroxidase, laccase and beta-glucosidase was isolated from culture extracts of the white rot basidiomycete Lentinula edodes. This protein complex showed a single protein band on native polyacrylamide gel electrophoresis (PAGE). On sodium dodecyl sulfate (SDS)-PAGE, however, it displayed three major bands and several additional minor bands ranging in size from 60 kDa to 180 kDa, suggesting it being a complex of six to eight different proteins. The molecular mass of this complex was estimated to be approximately 660 kDa from the elution position of gel filtration. This enzyme complex was effective in transforming environmentally persistent xenobiotics, pentachlorophenol and 2,5-dichlorophenol.
Makkar
R S
RS
National Food Research Institute, Tsukuba, Ibakari, Japan.
Tsuneda
A
A
Tokuyasu
K
K
Mori
Y
Y
D042845:Laccase; D008970:Molecular Weight; D010088:Oxidoreductases / Q000378:metabolism*; D010544:Peroxidases / Q000378:metabolism*; D020942:Shiitake Mushrooms / Q000201:enzymology*; D001617:beta-Glucosidase / Q000378:metabolism*
D010088:Oxidoreductases; D042845:Laccase; D010544:Peroxidases; C051129:manganese peroxidase; D001617:beta-Glucosidase