[Inhibition of voltage-activated outward delayed rectifier potassium channel currents in dorsal root ganglion neurons of rats by lead].
OBJECTIVE
To study the effect of lead on voltage-activated outward delayed rectifier potassium channel currents in acute isolated dorsal root ganglion (DRG) neurons of adult rats.


METHODS
Patch clamp technique was employed to record the whole cell of DRG in adult rats.


RESULTS
In the experiments, delayed rectifier (IK) potassium currents channel was recorded and activation was voltage-dependent. The threshold concentration of lead (Pb2+) for IK was 1 mumol/L, which reduced potassium current by (8.6 +/- 0.8)%, 4 mumol/L of Pb2+ reduced the current by (38.6 +/- 6.2)%, and 8 mumol/L of Pb2+ reduced the current by (63.0 +/- 5.1)%. The action of lead was rapid and its effect would be reversed rapidly to various extent by washing.


CONCLUSIONS
Higher outward delayed rectifier potassium channel currents, which could be reversed.
Xiao
H
H
Institute of Toxicology, Nanjing Medical University School of Public Health, Jiangsu 210029, China.
Dai
X
X
Mao
X
X
D000818:Animals; D002478:Cells, Cultured; D005260:Female; D005727:Ganglia, Spinal / Q000166:cytology*; D007854:Lead / Q000633:toxicity*; D008297:Male; D009474:Neurons / Q000187:drug effects* / Q000502:physiology; D018408:Patch-Clamp Techniques; D024642:Potassium Channels, Voltage-Gated / Q000187:drug effects* / Q000502:physiology; D051381:Rats
D024642:Potassium Channels, Voltage-Gated; D007854:Lead