Identification and isolation of a full-length clone of mouse GMFB (Gmfb), a putative intracellular kinase regulator, differentially expressed in telencephalon.
We identified new transcribed sequences, using a differential display paradigm to select genes expressed in proliferating neuroblasts from mouse telencephalon at 10 days of embryonic development. In this systematic search, we isolated a 361-bp partial 3' untranslated region (3' UTR) homologous to the 3' UTR of the human gene encoding a putative intracellular kinase regulator, glia maturation factor beta (GMFB). We cloned a full-length, 4,311-bp mouse cDNA containing a 270-bp 5' UTR, a 3,615-bp 3' UTR, and an open reading frame of 426 nucleotides encoding a putative 142 amino-acid protein, identical to human GMFB, with the exception of two amino acids. This 4.3-kb transcript is present in a variety of adult tissues and is developmentally regulated as shown by Northern blot analysis. Differential expression in telencephalon was demonstrated by quantification of radioactive relative RT-PCR and confirmed by in situ hybridization. The isolation of this full-length clone of mouse Gmfb should facilitate investigation of the intracellular mechanisms involved in the development of telencephalon.
Bourgeois
F
F
Neurogénétique, INSERM E9935, Hôpital Robert Debré, Paris, France.
Guimiot
F
F
Mas
C
C
Bulfone
A
A
Levacher
B
B
Moalic
J M
JM
Simonneau
M
M
D020413:3' Untranslated Regions / Q000032:analysis / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D020224:Expressed Sequence Tags; D018507:Gene Expression Regulation, Developmental; D020904:Glia Maturation Factor / Q000737:chemistry / Q000235:genetics* / Q000494:pharmacology; D006801:Humans; D017403:In Situ Hybridization; D051379:Mice; D008969:Molecular Sequence Data; D011494:Protein Kinases / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D016415:Sequence Alignment; D017385:Sequence Homology; D013687:Telencephalon / Q000166:cytology / Q000196:embryology / Q000201:enzymology / Q000378:metabolism*
D020413:3' Untranslated Regions; D018076:DNA, Complementary; D020904:Glia Maturation Factor; D012333:RNA, Messenger; D011494:Protein Kinases