Miniaturized real-time monitoring system for L-lactate and glucose using microfabricated multi-enzyme sensors.
A miniaturized on-line monitoring system for the detection of L-lactate and glucose is presented. The system is based on a microfabricated multi-enzyme silicon sensor chip with flow channels integrated on the chip. The sensors were fabricated in containment technology. They were characterized in test solutions. The cross-talking behaviour was investigated and was found to be practically negligible. The linear measurement ranges of both glucose and lactate sensors were large enough for most practical applications. As a result of the miniaturization the analyte consumption could be reduced to a few nmol min(-1). The system was equipped with a microdialysis probe whose recovery was 45% for lactate and 37% for glucose in test solutions using a flow rate of 3 microl min(-1). Lower flow rates of 0.5 microl min(-1) resulted in recoveries of over 90%. The long-term stability of the system was acceptable. Initial measurements have also been performed in vitro using human blood serum.
Perdomo
J
J
Centro de Investigaciones en Microelectrónica, Instituto Superior Politécnico Jose A. Echererria, Havana, Cuba.
Hinkers
H
H
Sundermeier
C
C
Seifert
W
W
Martínez Morell
O
O
Knoll
M
M
D015374:Biosensing Techniques / Q000295:instrumentation* / Q000706:statistics & numerical data; D001786:Blood Glucose / Q000032:analysis; D005947:Glucose / Q000032:analysis*; D006801:Humans; D066298:In Vitro Techniques; D019344:Lactic Acid / Q000032:analysis* / Q000097:blood; D017551:Microdialysis; D008991:Monitoring, Physiologic / Q000295:instrumentation; D009862:Online Systems; D012680:Sensitivity and Specificity; D012825:Silicon
D001786:Blood Glucose; D019344:Lactic Acid; D005947:Glucose; D012825:Silicon