Rapid diagnosis of Histoplasma capsulatum endocarditis using the AccuProbe on an excised valve.
Histoplasma capsulatum is an infrequent but serious cause of endocarditis. The definitive diagnosis requires culture, which may require a long incubation. We demonstrated the ability of the Histoplasma capsulatum AccuProbe to accurately identify this organism when applied directly on an excised valve that contained abundant yeast forms consistent with H. capsulatum.
Chemaly
R F
RF
Department of Clinical Pathology, Section of Clinical Microbiology, The Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA.
Tomford
J W
JW
Hall
G S
GS
Sholtis
M
M
Chua
J D
JD
Procop
G W
GW
D001021:Aortic Valve; D015342:DNA Probes; D004275:DNA, Ribosomal / Q000032:analysis; D004696:Endocarditis / Q000175:diagnosis* / Q000382:microbiology / Q000601:surgery; D006350:Heart Valve Prosthesis / Q000382:microbiology*; D006658:Histoplasma / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D006660:Histoplasmosis / Q000175:diagnosis* / Q000382:microbiology / Q000601:surgery; D006801:Humans; D008297:Male; D008875:Middle Aged; D012335:RNA, Ribosomal / Q000235:genetics; D011933:Reagent Kits, Diagnostic
D015342:DNA Probes; D004275:DNA, Ribosomal; D012335:RNA, Ribosomal; D011933:Reagent Kits, Diagnostic