Addition of ATP and MgCl2 to the preservation solution attenuates lung reperfusion injury following cold ischemia.
BACKGROUND
In lung transplantation, reperfusion injury following cold ischemia is one of the crucial problems for recipients.


OBJECTIVE
We evaluated the protective effect of adding a combination of ATP and MgCl2 to the preservation solution against lung reperfusion injury following cold ischemia.


METHODS
Using an isolated rat lung perfusion model with fresh rat blood as the perfusate, the rats were divided into five groups (n = 6). In the fresh group, the study lungs were flushed with phosphate-buffered saline (PBS), then immediately reperfused for 120 min. In the control group, the study lungs were flushed with PBS, then cold ischemia was induced for 9 h (4 degrees C), after which reperfusion was performed. In the other three groups, the protocols were the same as for the control group except that ATP and/or MgCl2 were added to the PBS: ATP group (100 microM ATP), MgCl2 group (100 microM MgCl2) and ATP + MgCl2 group (100 microM ATP + 100 microM MgCl2).


RESULTS
In the ATP + MgCl2 group, the intrapulmonary shunt fraction, peak airway pressure and wet to dry lung weight ratio were significantly lower than those in the control group. No improvement was observed in the ATP or MgCl2 groups. Histological examination supported these physiological results. In all groups, flush time and lipid peroxide levels in the lungs after cold ischemia did not show any significant differences.


CONCLUSIONS
The addition of ATP and MgCl2 to the preservation solution attenuated reperfusion injury following cold ischemia in rat lungs.
Hirata
T
T
Department of Thoracic Surgery, Faculty of Medicine, Kyoto University, Kyoto, Japan. thirata@kuhp.kyoto-u.ac.jp
Fukuse
T
T
Ishikawa
S
S
Miyahara
R
R
Wada
H
H
D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000818:Animals; D066298:In Vitro Techniques; D015636:Magnesium Chloride / Q000494:pharmacology*; D008297:Male; D023421:Models, Animal; D019188:Organ Preservation Solutions / Q000737:chemistry*; D051381:Rats; D011917:Rats, Inbred Lew; D015427:Reperfusion Injury / Q000517:prevention & control*
D019188:Organ Preservation Solutions; D015636:Magnesium Chloride; D000255:Adenosine Triphosphate