Organization and regulation of meta cleavage pathway genes for toluene and o-xylene derivative degradation in Pseudomonas stutzeri OX1.
Pseudomonas stutzeri OX1 meta pathway genes for toluene and o-xylene catabolism were analyzed, and loci encoding phenol hydroxylase, catechol 2,3-dioxygenase, 2-hydroxymuconate semialdehyde dehydrogenase, and 2-hydroxymuconate semialdehyde hydrolase were mapped. Phenol hydroxylase converted a broad range of substrates, as it was also able to transform the nongrowth substrates 2,4-dimethylphenol and 2,5-dimethylphenol into 3,5-dimethylcatechol and 3,6-dimethylcatechol, respectively, which, however, were not cleaved by catechol 2,3-dioxygenase. The identified gene cluster displayed a gene order similar to that of the Pseudomonas sp. strain CF600 dmp operon for phenol catabolism and was found to be coregulated by the tou operon activator TouR. A hypothesis about the evolution of the toluene and o-xylene catabolic pathway in P. stutzeri OX1 is discussed.
Arenghi
F L
FL
Dipartimento di Genetica e di Biologia dei Microrganismi, Università degli Studi di Milano, Milano, Italy.
Berlanda
D
D
Galli
E
E
Sello
G
G
Barbieri
P
P
D001673:Biodegradation, Environmental; D002874:Chromosome Mapping; D006899:Mixed Function Oxygenases / Q000235:genetics / Q000378:metabolism; D010105:Oxygenases / Q000235:genetics / Q000378:metabolism; D011549:Pseudomonas / Q000201:enzymology* / Q000235:genetics*; D014050:Toluene / Q000031:analogs & derivatives* / Q000378:metabolism*; D014992:Xylenes / Q000378:metabolism*
D014992:Xylenes; D014050:Toluene; C027804:2,4-dimethylphenol; D006899:Mixed Function Oxygenases; D010105:Oxygenases; C022336:phenol 2-monooxygenase; C026114:2-xylene