title: Evidence of increased endothelial cell turnover in brain arteriovenous malformations.
abstract: OBJECTIVE
  We hypothesized that human brain arteriovenous malformations (BAVMs) are nonstatic vascular lesions with active angiogenesis or vascular remodeling. To test this hypothesis, we assessed endothelial cell turnover in BAVMs.
  
  
  METHODS
  We identified nonresting endothelial cells by use of immunohistochemistry for the Ki-67 antigen. From archived paraffin blocks, we selected BAVM vessels without intravascular thrombosis or embolic material in areas nonadjacent to the nidus edge. For controls, we used 50- to 100-microm diameter cortical vessels from temporal lobe cortex removed for epilepsy treatment. The Ki-67 index was calculated as a percentage of Ki-67-positive endothelial cells. The data were analyzed by the nonparametric Mann-Whitney test and reported as mean +/- standard deviation.
  
  
  RESULTS
  Thirty-seven specimens that met the above criteria were selected. There were 26 +/- 15 vessels counted in each BAVM specimen versus 18 +/- 5 in each control cortex (n = 5). The mean Ki-67 index was higher for BAVM vessels than control cortical vessels (0.7 +/- 0.6 versus 0.1 +/- 0.2%; P = 0.005), which represented an approximately seven-fold increase in the number of nonresting endothelial cells. In the BAVM group, there was a trend for younger patients to have a wider variation and higher Ki-67 index than older patients; no trend was evident in the control group.
  
  
  CONCLUSIONS
  Compared with control vessels, BAVM vessels have higher endothelial cell turnover, which suggests the presence of active angiogenesis or vascular remodeling in BAVMs.
authors: Department of Anesthesia and Perioperative Care, University of California at San Francisco, 94110, USA.
authors: T
authors: T
authors: Hashimoto
authors: R
authors: R
authors: Mesa-Tejada
authors: C M
authors: CM
authors: Quick
authors: A W
authors: AW
authors: Bollen
authors: S
authors: S
authors: Joshi
authors: J
authors: J
authors: Pile-Spellman
authors: M T
authors: MT
authors: Lawton
authors: W L
authors: WL
authors: Young
mesh_terms: D000328:Adult; D001808:Blood Vessels / Q000378:metabolism / Q000473:pathology; D004730:Endothelium, Vascular / Q000378:metabolism / Q000473:pathology*; D004827:Epilepsy / Q000473:pathology; D005260:Female; D006801:Humans; D002538:Intracranial Arteriovenous Malformations / Q000473:pathology*; D019394:Ki-67 Antigen / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D013702:Temporal Lobe / Q000098:blood supply
chemical_list: D019394:Ki-67 Antigen