title: Analysis of IgG response to experimental infection with RH Toxoplasma gondii in goats.
abstract: The IgG response of goats experimentally infected with RH Toxoplasma gondii has been analysed using an indirect ELISA and Western-blot analysis. Specific IgG antibodies were first detected at 14 days post-inoculation (p.i.), reaching a peak by day 35 p.i. and showing slight fluctuations until the end of the experiment (91 p.i.). Specific IgG showed a reactivity over a whole range of peptides (125-24 kDa approximately), but the highest reactivity was observed against a group of antigens with a molecular weight between 34 and 28 kDa, in particular against a 30 kDa fraction which is considered to represent the major surface protein of T. gondii named p30 or SAG-1.
authors: Departamento de Patología Animal (Parasitología y Enfermedades Parasitarias), Facultad de Veterinaria de la Universidad de Las Palmas de Gran Canaria, Spain.
authors: M
authors: M
authors: Conde
authors: J M
authors: JM
authors: Caballero
authors: E
authors: E
authors: Rodríguez-Ponce
authors: A
authors: A
authors: Ruiz
authors: J
authors: J
authors: González
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000096:biosynthesis* / Q000097:blood; D015153:Blotting, Western / Q000662:veterinary; D016054:DNA, Protozoan / Q000737:chemistry / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D015511:Goat Diseases / Q000276:immunology* / Q000469:parasitology; D006041:Goats; D006651:Histocytochemistry / Q000662:veterinary; D007074:Immunoglobulin G / Q000096:biosynthesis* / Q000097:blood; D016133:Polymerase Chain Reaction / Q000662:veterinary; D014122:Toxoplasma / Q000235:genetics / Q000276:immunology*; D014124:Toxoplasmosis, Animal / Q000097:blood / Q000276:immunology* / Q000469:parasitology
chemical_list: D000913:Antibodies, Protozoan; D016054:DNA, Protozoan; D007074:Immunoglobulin G