Bacteriophage lambda-based expression vectors.
Bacteriophage lambda has been in use as a cloning vector for over 25 years, and has been used extensively as an expression vector. The efficiency of packaging and infection, and the simplicity of plaque screening are advantages of lambda as a cloning vector. A number of ingenious modifications help overcome the disadvantages associated with its mode of growth and its size. Some lambda vectors have been designed to be readily converted into plasmids or phagemids, and there are a variety of promoters and fusions that can be used to drive expression of foreign genes. Screening lambda libraries with antibodies or ligands is a powerful way of identifying novel genes.
Christensen
A C
AC
School of Biological Sciences, University of Nebraska, Lincoln, NE 68588-0118, USA. acc@biocomp.unl.edu
D010582:Bacteriophage lambda / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism; D018076:DNA, Complementary / Q000378:metabolism; D015723:Gene Library; D005821:Genetic Techniques; D005822:Genetic Vectors; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000378:metabolism
D018076:DNA, Complementary; D011993:Recombinant Fusion Proteins; D004247:DNA