Parasitological, pathological and functional studies on the effects of IFN alpha 2b in murine hepatic schistosomiasis.
The effects of different doses of Interferon alpha 2b (IFN alpha 2b), alone and in combination with praziquantel (PZQ), on hepatic schistosomiasis were tested. An experimental murine model of hepatic schistosomiasis was used. Four parameters were assessed; hepatic fibrosis by estimation of OH-proline content/g dry weight liver, hepatocyte proliferative activity by the PCNA/LI, schistosomal egg load by digesting parts of the liver by KOH and hepatocyte function by measuring parenchymal liver enzyme levels. IFN alpha 2b was found to increase hepatic fibrosis in a dose dependent manner both alone and in combination with PZQ. An augmentation of the regenerative activity of the liver was observed. A reduction in the number of the granulomas and egg counts was observed only when PZQ was added. However, no effect on the size of the granulomas was observed apart from the normal process of modulation. Caution should be exercised when treating patients with concomitant hepatic schistosomiasis and hepatitis with IFN alpha 2b as it increases both hepatocyte regenerative activity and hepatic fibrosis; two main components of cirrhosis.
Eissa
M M
MM
Department of Parasitology, Faculty of Medicine, Alexandria University, Egypt.
Baddour
N M
NM
D000818:Animals; D000871:Anthelmintics / Q000008:administration & dosage / Q000627:therapeutic use*; D004359:Drug Therapy, Combination; D000077190:Interferon alpha-2; D016898:Interferon-alpha / Q000008:administration & dosage / Q000627:therapeutic use*; D008109:Liver Diseases, Parasitic / Q000188:drug therapy* / Q000473:pathology; D008111:Liver Function Tests; D051379:Mice; D010270:Parasite Egg Count; D011223:Praziquantel / Q000008:administration & dosage / Q000627:therapeutic use*; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D011994:Recombinant Proteins; D012552:Schistosomiasis / Q000188:drug therapy* / Q000473:pathology
D000871:Anthelmintics; D000077190:Interferon alpha-2; D016898:Interferon-alpha; D018809:Proliferating Cell Nuclear Antigen; D011994:Recombinant Proteins; D011223:Praziquantel