title: Characterization of three infectious bronchitis virus isolates from China associated with proventriculus in vaccinated chickens.
abstract: Outbreaks of an avian disease in infectious bronchitis-vaccinated chickens in China have led to the characterization of coronaviral isolates Q1, J2, and T3, which were isolated from proventricular tissues of the affected young layer flocks. Serologic analysis revealed that they could induce high titers of infectious bronchitis virus (IBV) antibodies in inoculated specific-pathogen-free (SPF) chickens in indirect enzyme-linked immunosorbent assay but were not neutralized by antisera specific to the IBV serotype M41 and the Australian T strain. In a pathogenicity experiment, the clinical signs and related gross lesions resembling those of field outbreaks were reproduced in SPF chickens, and viruses were reisolated from the damaged tissues, including trachea, proventriculus, duodenum, and cecal tonsil. Sequence data demonstrated the complete S1 amino acid sequences of these isolates were almost identical despite recovery from geographically different areas in China and had 47.3%-82.3% similarity in comparison with the 47 published S1 sequences. On the basis of genotyping and limited serology, the three isolates, which were responsible for field outbreaks of the disease, might be a new IBV variant.
authors: Animal Health Biotechnology Laboratory, Institute of Molecular Agrobiology, National University of Singapore, Singapore.
authors: L
authors: L
authors: Yu
authors: Y
authors: Y
authors: Jiang
authors: S
authors: S
authors: Low
authors: Z
authors: Z
authors: Wang
authors: S J
authors: SJ
authors: Nam
authors: W
authors: W
authors: Liu
authors: J
authors: J
authors: Kwangac
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D001483:Base Sequence; D002642:Chick Embryo; D002645:Chickens; D002681:China; D018352:Coronavirus Infections / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D004196:Disease Outbreaks / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D005838:Genotype; D001351:Infectious bronchitis virus / Q000145:classification* / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D008969:Molecular Sequence Data; D011201:Poultry Diseases / Q000473:pathology / Q000821:virology*; D011531:Proventriculus / Q000821:virology*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D012698:Serologic Tests / Q000662:veterinary; D013047:Specific Pathogen-Free Organisms
chemical_list: D000914:Antibodies, Viral