Effects of 22K or 20K human growth hormone on lipolysis, leptin production in adipocytes in the presence and absence of human growth hormone binding protein.
OBJECTIVE
We studied the effects of human growth hormone (hGH) on leptin production and lipolysis stimulation in the presence or absence of human growth hormone binding protein (hGHBP) using 3T3- L1-hGHR adipocytes which efficiently express human growth hormone receptor.


CONCLUSIONS
It was clarified that (1) hGH decreases leptin secretion after hGH-induced lipolysis stimulation, and (2) the reduction of leptin production and lipolysis stimulation by 22K hGH was attenuated with hGHBP, whereas that by 20K hGH, which is a naturally occurring isoform of 22K hGH, was not affected with hGHBP.
Asada
N
N
Pharmaceuticals Group, Life Science Laboratory, Mitsui Chemicals, Inc., Mobara-shi, Chiba, Japan. noriaki.asada@mitsui-chem.co.jp
Takahashi
Y
Y
Honjo
M
M
D016475:3T3 Cells; D017667:Adipocytes / Q000187:drug effects* / Q000378:metabolism*; D000818:Animals; D002352:Carrier Proteins / Q000494:pharmacology*; D005230:Fatty Acids, Nonesterified / Q000378:metabolism; D015870:Gene Expression; D019382:Human Growth Hormone / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D020738:Leptin / Q000096:biosynthesis*; D008066:Lipolysis / Q000187:drug effects*; D051379:Mice; D008970:Molecular Weight; D054411:Receptors, Leptin; D011986:Receptors, Somatotropin / Q000235:genetics
D002352:Carrier Proteins; D005230:Fatty Acids, Nonesterified; C515448:LEPR protein, human; D020738:Leptin; D054411:Receptors, Leptin; D011986:Receptors, Somatotropin; C515447:leptin receptor, mouse; D019382:Human Growth Hormone; C051815:somatotropin-binding protein