title: Synthesis of 5'-deoxy-5'-thioguanosine-5'-monophosphorothioate and its incorporation into RNA 5'-termini.
abstract: [figure: see text] 5'-Deoxy-5'-thioguanosine-5'-monophosphorothioate (GSMP) was synthesized in four steps with 35% overall yield. GSMP serves as a good substrate for in vitro transcription with T7 RNA polymerase to yield 5'-GSMP-RNA, which was converted to 5'-HS-RNA by dephosphorylation with alkaline phosphatase. The thiol-reactive agents can be efficiently introduced into the 5'-terminus of RNA.
authors: Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA. biliang.zhang@umassmed.edu
authors: B
authors: B
authors: Zhang
authors: Z
authors: Z
authors: Cui
authors: L
authors: L
authors: Sun
mesh_terms: D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D006151:Guanosine / Q000031:analogs & derivatives / Q000138:chemical synthesis* / Q000737:chemistry / Q000493:pharmacokinetics*; D007202:Indicators and Reagents; D012367:RNA, Viral / Q000096:biosynthesis* / Q000737:chemistry; D013379:Substrate Specificity; D013873:Thionucleotides / Q000138:chemical synthesis* / Q000737:chemistry / Q000493:pharmacokinetics*; D014764:Viral Proteins
chemical_list: C432004:5'-deoxy-5'-thioguanosine-5'-monophosphorothioate; D007202:Indicators and Reagents; D012367:RNA, Viral; D013873:Thionucleotides; D014764:Viral Proteins; D006151:Guanosine; C062210:bacteriophage T7 RNA polymerase; D012321:DNA-Directed RNA Polymerases