A high-density poly(ethylene glycol) polymer brush for immobilization on glass-type surfaces.
Label-free heterogeneous phase detection critically depends on the properties of the interfacial layer. We have obtained high-density monomolecular poly(ethylene glycol) (PEG) layers by solvent-free coupling of homo-bifunctional PEGs (2,000 g/mol) at 75 degrees C to silica surfaces silanized with glycidyloxipropyltrimethoxysilane (GOPTS). Characterization by ellipsometry and contact angles revealed that PEG layers up to 3.4 ng/mm2 with low roughness and flexibility were obtained. Specific and non-specific binding at these PEG surfaces was monitored by reflectometric interference spectroscopy (RIfS). No significant non-specific adsorption upon incubation of 1 mg/ml ovalbumin was detectable (< 10 pg/mm2), and 150 pg/mm2 upon incubation of 10% calf serum, less than 10% of the amount adsorbed to the solely silanized surfaces. The terminal functional groups of the PEG layers were utilized to couple ligands and a protein. Specific protein interaction with these immobilized compounds was detected with saturation loadings in the range of protein monolayers (2-4 ng/mm2). The excellent functional properties, the high stability of the layers, the generic and practical coupling procedure and the versatility for immobilizing compounds of very different functionality make these PEG layers very attractive for application in label-free detection with silica or metal-oxide based transducers.
Piehler
J
J
Institut für Physikalische Chemie, Tübingen, Germany. piehler@mailer.uni-marburg.de
Brecht
A
A
Valiokas
R
R
Liedberg
B
B
Gauglitz
G
G
D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D004355:Drug Stability; D005898:Glass; D066298:In Vitro Techniques; D008024:Ligands; D010047:Ovalbumin; D011092:Polyethylene Glycols; D011485:Protein Binding; D012822:Silicon Dioxide; D013499:Surface Properties
D008024:Ligands; D011092:Polyethylene Glycols; D012822:Silicon Dioxide; D010047:Ovalbumin