Primary structure and developmental expression of zebrafish sodium channel Na(v)1.6 during neurogenesis.
A zebrafish sodium channel cDNA encoding a 1949-amino acid polypeptide, Na(v)1.6, was isolated. Two transcripts were detected in zebrafish adult brain but not in cardiac or skeletal muscle. The RNase protection analysis confirmed the neural specificity of zebrafish Na(v)1.6 24 hours postfertilization (hpf) Na(v)1.6 was expressed in the trigeminal ganglion, anterior and posterior lateral line ganglia, rhombomeres, and Rohon-Beard neurons. This preferential localization suggests that Na(v)1.6 plays an important role in tactile sensitivity. The abundance of zebrafish Na(v) 1.6 mRNA in the central and peripheral nervous systems increased markedly between 48 and 72 hpf, during the maturation of the nervous system.
Tsai
C W
CW
Institute of Neuroscience, School of Life Science, National Yang-Ming University, Taipei, Taiwan, ROC.
Tseng
J J
JJ
Lin
S C
SC
Chang
C Y
CY
Wu
J L
JL
Horng
J F
JF
Tsay
H J
HJ
D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000378:metabolism; D003001:Cloning, Molecular; D004625:Embryo, Nonmammalian / Q000378:metabolism; D005727:Ganglia, Spinal / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D009475:Neurons, Afferent / Q000737:chemistry / Q000378:metabolism*; D010802:Phylogeny; D011487:Protein Conformation; D012165:Retinal Ganglion Cells / Q000737:chemistry / Q000378:metabolism; D015222:Sodium Channels / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D013477:Superior Colliculi / Q000737:chemistry / Q000378:metabolism; D014110:Touch; D015027:Zebrafish / Q000196:embryology / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029961:Zebrafish Proteins
D015222:Sodium Channels; D029961:Zebrafish Proteins; C430124:scn8ab protein, zebrafish