title: Differential effects of parainfluenza virus type 3 on human monocytes and dendritic cells.
abstract: To understand the lack of protective immunity observed after infection with parainfluenza virus type 3 (PIV3), we tested the effect of the virus on human monocytes and monocyte-derived immature dendritic cells (DCs). Expression of viral antigens on the cell surfaces correlated with replication of the virus, which was marginal in monocytes but extremely efficient in DCs. The virus increased monocyte survival at least in part through the production of granulocyte-macrophage colony-stimulating factor but, in contrast, accelerated DC apoptosis. In addition, PIV3 infection failed to activate monocytes but induced maturation of DCs with increased expression of CD54, HLA-DR, CD86, and CD83 and production of bioactive IL-12. However, PIV3-infected DCs demonstrated low stimulatory properties in DC-T cell cocultures, a finding that could not be attributed to the production of infectious virus or IL-10. These results demonstrate for the first time that PIV3 dramatically modifies the survival and/or the function of antigen-presenting cells and might therefore prevent the development of efficient antiviral immune responses.
authors: Centre d'Immunologie Pierre Fabre, 5, Av. Napoléon III, St Julien en Genevois, 74 164, France. helene.pltnicky..gilquin@pierre-fabre.com
authors: H
authors: H
authors: Plotnicky-Gilquin
authors: D
authors: D
authors: Cyblat
authors: J P
authors: JP
authors: Aubry
authors: Y
authors: Y
authors: Delneste
authors: A
authors: A
authors: Blaecke
authors: J Y
authors: JY
authors: Bonnefoy
authors: N
authors: N
authors: Corvaïa
authors: P
authors: P
authors: Jeannin
mesh_terms: D015703:Antigens, CD / Q000032:analysis; D000956:Antigens, Viral / Q000032:analysis; D017209:Apoptosis; D051940:B7-2 Antigen; D002454:Cell Differentiation; D002470:Cell Survival; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology / Q000821:virology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000032:analysis / Q000096:biosynthesis; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D007136:Immunoglobulins / Q000032:analysis; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis; D018664:Interleukin-12 / Q000032:analysis; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000821:virology*; D008562:Membrane Glycoproteins / Q000032:analysis; D010224:Parainfluenza Virus 3, Human / Q000502:physiology*; D012333:RNA, Messenger / Q000096:biosynthesis; D014779:Virus Replication; D000097802:CD83 Antigen
chemical_list: D015703:Antigens, CD; D000956:Antigens, Viral; D051940:B7-2 Antigen; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006684:HLA-DR Antigens; D007136:Immunoglobulins; D018799:Intercellular Adhesion Molecule-1; D018664:Interleukin-12; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; D000097802:CD83 Antigen; C497964:CD86 protein, human