Conversion of cyclic nonaketides to lovastatin and compactin by a lovC deficient mutant of Aspergillus terreus.
Investigation of the post-PKS biosynthetic steps to the cholesterol-lowering agent lovastatin (1) using an Aspergillus terreus strain with a disrupted lovC gene, which is essential for formation of 4a,5-dihydromonacolin L (3), shows that 7 and 3 are precursors to 1, and demonstrates that lovastatin diketide synthase (lovF protein) does not require lovC.
Auclair
K
K
Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada T6G 2G2.
Kennedy
J
J
Hutchinson
C R
CR
Vederas
J C
JC
D000924:Anticholesteremic Agents / Q000378:metabolism; D001230:Aspergillus / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D005656:Fungal Proteins; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors / Q000378:metabolism; D008148:Lovastatin / Q000031:analogs & derivatives* / Q000096:biosynthesis*; D009097:Multienzyme Complexes / Q000235:genetics* / Q000378:metabolism
D000924:Anticholesteremic Agents; D005656:Fungal Proteins; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D009097:Multienzyme Complexes; C442940:lovC protein, Aspergillus terreus; C012258:mevastatin; D008148:Lovastatin