DL-Buthionine-S,R-sulfoximine affects intestinal alkaline phosphatase activity.
The susceptibility of intestinal alkaline phosphatase to DL-buthionine-S,R-sulfoximine was investigated in chicks fed a commercial diet. The results show that DL-buthionine-S,R-sulfoximine produced inhibition of intestinal alkaline phosphatase activity. This effect showed dose- and time-dependency and it was caused by either in vivo DL-buthionine-S,R- sulfoximine administration or in vitro DL-buthionine-S,R-sulfoximine incubation with villus tip enterocytes. DL-Buthionine-S,R-sulfoximine did not act directly on intestinal alkaline phosphatase but it provoked glutathione depletion which led to changes in the redox state of the enterocyte as shown by the production of free hydroxyl radicals and an incremental increase in the carbonyl content of proteins. The reversibility of the buthionine sulfoximine effect on intestinal alkaline phosphatase was proved by addition of glutathione monoester to the duodenal loop.
Marchionatti
A
A
Cátedra de Química Biológica, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, C.C. 35, Suc. 16, 5016, Córdoba, Argentina.
Alisio
A
A
Díaz de Barboza
G
G
Baudino
V
V
Tolosa de Talamoni
N
N
D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D000963:Antimetabolites / Q000494:pharmacology; D019328:Buthionine Sulfoximine / Q000008:administration & dosage / Q000494:pharmacology*; D002118:Calcium / Q000493:pharmacokinetics*; D002645:Chickens / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004386:Duodenum / Q000201:enzymology; D004578:Electron Spin Resonance Spectroscopy; D005609:Free Radicals; D005978:Glutathione / Q000502:physiology; D066298:In Vitro Techniques; D007274:Injections, Intraperitoneal; D007408:Intestinal Absorption / Q000502:physiology; D007413:Intestinal Mucosa / Q000187:drug effects / Q000201:enzymology*; D007700:Kinetics; D018384:Oxidative Stress; D013054:Spectrophotometry, Atomic; D013997:Time Factors
D000963:Antimetabolites; D005609:Free Radicals; D019328:Buthionine Sulfoximine; D000469:Alkaline Phosphatase; D005978:Glutathione; D002118:Calcium