title: Isolation of symlandine from the roots of common comfrey (Symphytum officinale) using countercurrent chromatography.
abstract: Three pyrrolizidine alkaloids, symlandine, symphytine, and echimidine (1-3), were isolated from the roots of Symphytum officinale using a one-step countercurrent chromatography procedure. The structures of 1-3 were confirmed by several spectroscopic techniques including 2D NMR methods. This is the first description of the separation of symlandine (1) from its stereoisomer, symphytine (2).
authors: Chemistry and Life Sciences, Research Triangle Institute, 3040 Cornwallis Road, Research Triangle Park, North Carolina 27709, USA.
authors: N C
authors: NC
authors: Kim
authors: N H
authors: NH
authors: Oberlies
authors: D R
authors: DR
authors: Brine
authors: R W
authors: RW
authors: Handy
authors: M C
authors: MC
authors: Wani
authors: M E
authors: ME
authors: Wall
mesh_terms: D003377:Countercurrent Distribution; D009682:Magnetic Resonance Spectroscopy; D019684:Magnoliopsida / Q000737:chemistry*; D008956:Models, Chemical; D018517:Plant Roots / Q000737:chemistry; D011763:Pyrrolizidine Alkaloids / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D011763:Pyrrolizidine Alkaloids; C433586:symlandine