Lack of neighborhood effects from a transcriptionally active phosphoglycerate kinase-neo cassette located between the murine beta-major and beta-minor globin genes.
For the treatment of beta-globin gene defects, a homologous recombination-mediated gene correction approach would provide advantages over random integration-based gene therapy strategies. However, "neighborhood effects" from retained selectable marker genes in the targeted locus are among the key issues that must be taken into consideration for any attempt to use this strategy for gene correction. An Ala-to-Ile mutation was created in the beta6 position of the mouse beta-major globin gene (beta(6I)) as a step toward the development of a murine model system that could serve as a platform for therapeutic gene correction studies. The marked beta-major gene can be tracked at the level of DNA, RNA, and protein, allowing investigation of the impact of a retained phosphoglycerate kinase (PGK)-neo cassette located between the mutant beta-major and beta-minor globin genes on expression of these 2 neighboring genes. Although the PGK-neo cassette was expressed at high levels in adult erythroid cells, the abundance of the beta(6I) mRNA was indistinguishable from that of the wild-type counterpart in bone marrow cells. Similarly, the output from the beta-minor globin gene was also normal. Therefore, in this specific location, the retained, transcriptionally active PGK-neo cassette does not disrupt the regulated expression of the adult beta-globin genes. (Blood. 2001;98:65-73)
Kaufman
R M
RM
Department of Pathology/Laboratory Medicine, the Division of Oncology, Section of Stem Cell Biology, Washington University School of Medicine, St Louis, MO, USA.
Lu
Z H
ZH
Behl
R
R
Holt
J M
JM
Ackers
G K
GK
Ley
T J
TJ
D000818:Animals; D001853:Bone Marrow / Q000378:metabolism; D004912:Erythrocytes / Q000378:metabolism; D005786:Gene Expression Regulation; D018390:Gene Targeting; D005914:Globins / Q000235:genetics*; D006454:Hemoglobins / Q000378:metabolism; D066298:In Vitro Techniques; D051379:Mice; D008817:Mice, Mutant Strains; D016254:Mutagenesis, Insertional; D016297:Mutagenesis, Site-Directed; D010100:Oxygen / Q000378:metabolism; D010735:Phosphoglycerate Kinase / Q000235:genetics*; D012313:RNA / Q000378:metabolism; D011995:Recombination, Genetic; D014158:Transcription, Genetic / Q000235:genetics*
D006454:Hemoglobins; D012313:RNA; D005914:Globins; D010735:Phosphoglycerate Kinase; D010100:Oxygen