Molecular cloning and expression pattern of porcine myeloid DAP12-associating lectin-1.
DAP12 is an ITAM-bearing membrane protein that is associated with activating receptors in natural killer cells, granulocytes, macrophages, and monocytes. Myeloid DAP12-associating lectin-1 (MDL-1) is a type II membrane protein that associates with DAP12. In this study, we report the molecular cloning of two isoforms of porcine MDL-1 cDNA from pulmonary alveolar macrophages. The porcine MDL-1 short form has 165 amino acids and 70% sequence identity with the mouse MDL-1 short form. The long form has 20 more amino acids in the stalk region and 71% sequence identity with human MDL-1 and 67% with the mouse MDL-1 long form. Porcine MDL-1 contains a conserved lysine in the transmembrane domain. There are six putative N-linked glycosylation sites in the MDL-1 long form. MDL-1 transcripts were detected exclusively in macrophages and monocytes by RT-PCR. When transfected into 293 cells, porcine MDL-1 is expressed on the cell surface associated with DAP12.
Yim
D
D
Department of Microbiology and Immunology, Finch University of Health Sciences/The Chicago Medical School, 3333 Green Bay Road, North Chicago, Illinois 60064, USA.
Jie
H B
HB
Sotiriadis
J
J
Kim
Y S
YS
Kim
Y B
YB
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D037102:Lectins / Q000235:genetics*; D037181:Lectins, C-Type; D016676:Macrophages, Alveolar; D008969:Molecular Sequence Data; D009000:Monocytes; D020033:Protein Isoforms / Q000235:genetics; D011956:Receptors, Cell Surface / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D013552:Swine / Q000235:genetics*; D014018:Tissue Distribution
C509771:CLEC5A protein, human; C509772:Clec5a protein, mouse; D037102:Lectins; D037181:Lectins, C-Type; D020033:Protein Isoforms; D011956:Receptors, Cell Surface