[Study on interactions between TPO molecules tested with yeast two-hybrid system].
OBJECTIVE
To explore the interactions between TPO molecules and their locations.


METHODS
Full-length TPO, TPO(N) and TPO(C) were inserted respectively into the plasmids of the yeast two-hybrid system. Six different recombinant plasmids were generated. Every 2 recombinant plasmids were transformed into the yeast cell SFY526. The possible interactions between TPOs were then tested by the bioactivity of beta-galactosidase.


RESULTS
There are interactions between full-length TPO themselves TPO(N) themselves and TPO with TPO(N), but not between TPO(C)themselves, TPO with TPO(C) and TPO(N) with TPO(C).


CONCLUSIONS
Naturally, the molecular interaction domain of TPO may exist in the N terminus but not in the C terminus.
Wang
Z
Z
Institute of Virology, Chinese Academy of Preventive Medicine, Beijing 100052, China.
Yang
X
X
Tong
Y
Y
D013926:Thrombopoietin / Q000737:chemistry / Q000235:genetics*; D020798:Two-Hybrid System Techniques; D015003:Yeasts / Q000235:genetics
D013926:Thrombopoietin