title: Disruption and phenotypic analysis of six open reading frames from chromosome VII of Saccharomyces cerevisiae reveals one essential gene.
abstract: Six open reading frames (ORFs) located on chromosome VII of Saccharomyces cerevisiae (YGR205w, YGR210c, YGR211w, YGR241c, YGR243w and YGR244c) were disrupted in two different genetic backgrounds using short-flanking homology (SFH) gene replacement. Sporulation and tetrad analysis showed that YGR211w, recently identified as the yeast ZPR1 gene, is an essential gene. The other five genes are non-essential, and no phenotypes could be associated to their inactivation. Two of these genes have recently been further characterized: YGR241c (YAP1802) encodes a yeast adaptor protein and YGR244c (LSC2) encodes the beta-subunit of the succinyl-CoA ligase. For each ORF, a replacement cassette with long flanking regions homologous to the target locus was cloned in pUG7, and the cognate wild-type gene was cloned in pRS416.
authors: Escola Superior de Tecnologia da Saúde de Lisboa, R. José Carlos dos Santos 7, P-2725-256 Lisboa, Portugal.
authors: P
authors: P
authors: Guerreiro
authors: C
authors: C
authors: Rodrigues-Pousada
mesh_terms: D015825:Chromosomes, Fungal / Q000235:genetics*; D017353:Gene Deletion; D020043:Genes, Essential / Q000235:genetics*; D005800:Genes, Fungal / Q000235:genetics*; D016254:Mutagenesis, Insertional; D016366:Open Reading Frames / Q000235:genetics*; D010641:Phenotype; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development; D014170:Transformation, Genetic