Identification of a cadmium-induced gene in Mycobacterium bovis and Mycobacterium tuberculosis.
A 17-kDa protein (CadI) was induced by cadmium in Mycobacterium bovis and Mycobacterium tuberculosis. Comparison of the N-terminal sequence from M. bovis CadI with the annotated M. tuberculosis genome database identified Rv2641 as the encoding gene. Long and short promoter fragments from M. bovis cadI were fused to the lacZ reporter gene in pYUB76. Only the long fragment directed cadmium-inducible activity when electroporated into M. bovis. The cadI promoter has potential for both constitutive and inducible expression studies in M. bovis and M. tuberculosis.
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AgResearch, Wallaceville Animal Research Centre, P.O. Box 40063, Upper Hutt, New Zealand.
Wilson
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Collins
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D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics / Q000302:isolation & purification; D002104:Cadmium / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009163:Mycobacterium bovis / Q000187:drug effects* / Q000235:genetics; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000187:drug effects; D017386:Sequence Homology, Amino Acid
D001426:Bacterial Proteins; C434314:CadI protein, Mycobacterium; D002104:Cadmium