Use of a reverse dot blot procedure to identify the presence of multiple serovars in Chlamydia trachomatis urogenital infection.
Epidemiologic research requires identification of Chlamydia trachomatis serovars and detection of mixed infection. Antibody-based serotyping is unworkable when specimens are urine or vaginal swabs. We developed a reverse dot blot (RDB) to screen for multiple serotypes in these specimens. RDB yielded the predicted results on all artificially mixed samples and on seven of eight clinically mixed samples.
Stothard
D R
DR
Department of Medicine, Division of Infectious Diseases, Indiana University School of Medicine, Indianapolis, Indiana 46202, USA. dstothar@iupui.edu
D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D001483:Base Sequence; D002690:Chlamydia Infections / Q000382:microbiology*; D002692:Chlamydia trachomatis / Q000145:classification* / Q000235:genetics; D005260:Female; D052776:Female Urogenital Diseases / Q000382:microbiology*; D006801:Humans; D007223:Infant; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D018272:Porins; D012680:Sensitivity and Specificity; D012703:Serotyping
D001425:Bacterial Outer Membrane Proteins; D009841:Oligonucleotides; D018272:Porins; C071677:omp1 protein, Chlamydia trachomatis