Protein peroxidation processes in bovine retained and not-retained placenta.
Proteins as well as lipids and other biologically active substances may undergo peroxidative damage caused by reactive oxygen species (ROS). The aim of this study was the determination of this damage in cases of retained (RFM) and not-retained bovine foetal membranes. The levels of thiol groups were measured spectrophotometrically. Tryptophan, formylokinurenine and bityrosine bridges were determined using spectrofluorimetric methods. All parameters were determined in placental maternal and foetal homogenates collected from cows divided into six groups as follows: A, pre-term caesarian section without RFM; B, pre-term caesarian section with RFM; C, term caesarian section without RFM; D, term caesarian section with RFM; E, spontaneous delivery without RFM; F, spontaneous delivery with RFM. All parameters except tryptophan showed statistically significant differences between the maternal and foetal parts of the placenta. The concentrations of the thiol groups were lower in RFM than in not-RFM cases, whereas formylokinurenine and bityrosine concentrations showed the opposite relationship. Tryptophan levels were lower in cases of RFM rather than not-RFM samples but statistically significant differences occurred in groups C and D and the foetal part of groups E and F. Further experiments are necessary to determine the oxidative status of other biologically active substances in cases of retained bovine placenta.
Kankofer
M
M
Department of Biochemistry, Faculty of Veterinary Medicine, Agricultural University, 20-123 Lublin, Lubartowska 58 a, Poland.
D000818:Animals; D016022:Case-Control Studies; D002417:Cattle / Q000378:metabolism*; D002418:Cattle Diseases / Q000378:metabolism*; D005260:Female; D007736:Kynurenic Acid / Q000031:analogs & derivatives / Q000378:metabolism; D010920:Placenta / Q000378:metabolism*; D018457:Placenta, Retained / Q000378:metabolism / Q000662:veterinary*; D011247:Pregnancy; D011506:Proteins / Q000378:metabolism*; D013438:Sulfhydryl Compounds / Q000378:metabolism; D014364:Tryptophan / Q000378:metabolism; D014443:Tyrosine / Q000031:analogs & derivatives / Q000378:metabolism
D011506:Proteins; D013438:Sulfhydryl Compounds; D014443:Tyrosine; D014364:Tryptophan; C007543:dityrosine; D007736:Kynurenic Acid