Decrease in Cryptosporidium parvum oocyst infectivity in vitro by using the membrane filter dissolution method for recovering oocysts from water samples.
Exposure of Cryptosporidium parvum oocysts to solutions used for cellulose acetate membrane (CAM) dissolution filtration reduced their infectivity in HCT-8 cells. Ethanol (95% [vol/vol] and 70% [vol/vol]) alone and short exposure times to acetone decreased infectivity. These findings contrast with similar experiments using excystation assays and infectivity in mice.
Carreno
R A
RA
Department of Environmental Biology, University of Guelph, Guelph, Ontario, Canada N1G 2W1.
Pokorny
N J
NJ
Weir
S C
SC
Lee
H
H
Trevors
J T
JT
D000818:Animals; D002460:Cell Line; D002482:Cellulose / Q000031:analogs & derivatives; D003457:Cryptosporidiosis / Q000469:parasitology; D016785:Cryptosporidium parvum / Q000254:growth & development* / Q000302:isolation & purification / Q000472:pathogenicity*; D005374:Filtration / Q000379:methods; D006801:Humans; D008567:Membranes, Artificial; D014867:Water / Q000469:parasitology*
D008567:Membranes, Artificial; D014867:Water; C005062:acetylcellulose; D002482:Cellulose