title: Effectiveness of manual cleaning and disinfection of gastroendoscopes with 3% glutaraldehyde for decreasing risk of transmission of hepatitis C virus.
abstract: OBJECTIVE
  The effectiveness of manual cleaning and disinfection of gastroendoscopes with 3% glutaraldehyde in decreasing the risk of transmission of hepatitis C virus (HCV) was examined.
  
  
  METHODS
  Gastroendoscopes used for treatment of endoscopic esophageal variceal ligation in patients with HCV infection were manually cleaned and disinfected with 3% glutaraldehyde (n = 25), 2% glutaraldehyde (n = 17), or 0.1% benzethonium chloride (n = 25). Samples were obtained by pouring 20 ml of 0.9% NaCl solution into the biopsy-suction channel of the scope before and after cleaning and disinfection. HCV was detected with the polymerase chain reaction.
  
  
  RESULTS
  Of the 25 scopes in 3% glutaraldehyde group, nine (36%) were positive for HCV before cleaning and disinfection, but all became negative after cleaning and disinfection; the difference was statistically significant (p < 0.01). In contrast, in 2% glutaraldehyde group and in the routine cleaning group, there were no significant differences in the number of positive samples between before and after cleaning and disinfection.
  
  
  CONCLUSIONS
  Manual cleaning and disinfection of gastroscopes with 3% glutaraldehyde is useful for decreasing the risk of transmission of HCV among patients.
authors: Department of Gastrointestinal Oncology, Osaka Medical Center for Cancer and Cardiovascular Diseases, Japan.
authors: N
authors: N
authors: Sakai
authors: M
authors: M
authors: Tatsuta
authors: H
authors: H
authors: Iishi
authors: H
authors: H
authors: Yano
authors: S
authors: S
authors: Osaka
authors: A
authors: A
authors: Aoki
mesh_terms: D001558:Benzethonium / Q000494:pharmacology; D004202:Disinfectants / Q000494:pharmacology*; D004932:Esophageal and Gastric Varices / Q000821:virology; D005260:Female; D020699:Gastroscopes / Q000821:virology*; D005976:Glutaral / Q000494:pharmacology*; D006526:Hepatitis C / Q000635:transmission*; D006801:Humans; D008297:Male; D008875:Middle Aged
chemical_list: D004202:Disinfectants; D001558:Benzethonium; D005976:Glutaral