A recombinant Semliki Forest virus particle vaccine encoding the prME and NS1 proteins of louping ill virus is effective in a sheep challenge model.
This study has examined the efficacy following intramuscular administration of a recombinant Semliki Forest virus (rSFV) vaccine, encoding the prME and NS1 proteins of louping ill virus (LIV), in sheep. Administration of rSFV-LIV vaccine resulted in transient detection at the injection site and draining lymph node only and no dissemination to distal sites. In addition, the recombinant vaccine offered complete protection against subcutaneous challenge with LIV, and partial protection following intranasal administration of LIV. Protected animals had no pathological changes normally associated with LIV infection, and had developed high antibody titres. In contrast, the two animals not protected exhibited classical clinical signs and neuropathological lesions of LIV infection. These findings indicate that rSFV-based vaccines have the potential to be developed as effective prototype vaccines for LIV.
Morris-Downes
M M
MM
Department of Microbiology, Moyne Institute of Preventive Medicine, Trinity College, Dublin 2, Ireland.
Sheahan
B J
BJ
Fleeton
M N
MN
Liljeström
P
P
Reid
H W
HW
Atkins
G J
GJ
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004669:Encephalitis Viruses, Tick-Borne / Q000235:genetics* / Q000276:immunology*; D004675:Encephalitis, Tick-Borne / Q000276:immunology / Q000473:pathology / Q000517:prevention & control / Q000662:veterinary; D005822:Genetic Vectors; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012672:Semliki forest virus / Q000235:genetics* / Q000276:immunology*; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology / Q000473:pathology / Q000517:prevention & control; D014614:Vaccines, Synthetic / Q000235:genetics / Q000276:immunology / Q000494:pharmacology; D017361:Viral Nonstructural Proteins / Q000235:genetics* / Q000276:immunology*; D015678:Viral Structural Proteins / Q000235:genetics* / Q000276:immunology*; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology / Q000494:pharmacology
D000914:Antibodies, Viral; D017931:DNA Primers; D014614:Vaccines, Synthetic; D017361:Viral Nonstructural Proteins; D015678:Viral Structural Proteins; D014765:Viral Vaccines