title: Modulation of potassium current and calcium influx by somatostatin in rod bipolar cells isolated from the rabbit retina via sst2 receptors.
abstract: Somatostatin (somatotropin release-inhibiting factor, SRIF) receptor subtypes are expressed by several retinal neurons, suggesting that SRIF acts at multiple levels of the retinal circuitry, although functional data on this issue are scarce. Of the SRIF receptors, the sst2A isoform is expressed by rod bipolar cells (RBCs) of the rabbit retina, and in isolated RBCs we studied the role of sst2 receptors in modulating both K+ current (IK) and the intracellular free [Ca2+] ([Ca2+]i) using both voltage-clamp and Ca2+-imaging techniques. SRIF and octreotide (a SRIF agonist that binds to sst2 receptors) inhibited that component of IK corresponding to the activation of large-conductance, Ca2+- and voltage-dependent K+ channels (IBK) and reduced the K+-induced [Ca2+]i accumulation, suggesting that SRIF effects on IBK may have been secondary to inhibition of Ca2+ channels. Octreotide effects on IBK or on [Ca2+]i accumulation were prevented by RBC treatment with L-Tyr8-Cyanamid 154806, a novel sst2 receptor antagonist, indicating that SRIF effects were mediated by sst2 receptor activation. The present data indicate that SRIF may modulate the information flow through second-order retinal neurons via an action predominantly at sst2 receptors, contribute to the proposition that SRIF be added to the growing list of retinal neuromodulators, and suggest that one of its possible roles in the retina is to regulate transmitter release from RBCs.
authors: Dipartimento di Fisiologia e Biochimica G. Moruzzi, Università di Pisa, Italy.
authors: C
authors: C
authors: Petrucci
authors: V
authors: V
authors: Resta
authors: F
authors: F
authors: Fieni
authors: A
authors: A
authors: Bigiani
authors: P
authors: P
authors: Bagnoli
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D004594:Electrophysiology; D015282:Octreotide / Q000494:pharmacology; D018408:Patch-Clamp Techniques; D011817:Rabbits; D017481:Receptors, Somatostatin / Q000819:agonists / Q000502:physiology*; D017948:Retinal Rod Photoreceptor Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D013004:Somatostatin / Q000494:pharmacology*
chemical_list: D017481:Receptors, Somatostatin; D013004:Somatostatin; C093733:somatostatin receptor 2; D015282:Octreotide; D002118:Calcium