Monitoring signal transduction in cancer: from chips to fish.
The microarray format of RNA transcript analysis should provide new clues to carcinogenic processes. Because of the complex and heterogeneous nature of most tumor samples, histochemical techniques, particularly RNA fluorescent in situ hybridization (FISH), are required to test the predictions from microarray expression experiments. Here we describe our approach to verify new microarray data by examining RNA expression levels of five to seven different transcripts in a very few cells via FISH. (J Histochem Cytochem 49:925-926, 2001)
Lersch
R A
RA
Life Science Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA. ralersch@lbl.gov
Fung
J
J
Hsieh
H B
HB
Smida
J
J
Weier
H U
HU
D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D005583:Fourier Analysis; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000295:instrumentation / Q000379:methods*; D008856:Microscopy, Fluorescence; D008857:Microscopy, Interference; D009369:Neoplasms / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D011505:Protein-Tyrosine Kinases / Q000235:genetics / Q000378:metabolism; D012334:RNA, Neoplasm / Q000378:metabolism; D012815:Signal Processing, Computer-Assisted; D015398:Signal Transduction; D013050:Spectrometry, Fluorescence
D014408:Biomarkers, Tumor; D012334:RNA, Neoplasm; D011505:Protein-Tyrosine Kinases