Detection of kinetoplast DNA of Trypanosoma cruzi from dried feces of triatomine bugs by PCR.
It is important to clarify the distribution of infected triatomine bugs in the endemic area of Chagas' disease for proper control. In the present study, we tried to detect T. cruzi kinetoplast DNA by PCR from dried triatomine feces collected from the house wall of an endemic area to assess the distribution of infected bugs more easily. The primers (P35/P36) were chosen to amplify the conserved region within the minirepeats of T. cruzi kinetoplast minicircle DNA. The kinetoplast DNA of T. cruzi could be actually detected in the dried feces collected from the wall of a brick-built house in Santa Cruz, Bolivia. Next, we examined the stability of T. cruzi kinetoplast DNA in the feces exposed to artificial environments. T. cruzi DNA was also detected by PCR in the feces left for 26 weeks at 25 degrees C and in those left for 4 weeks at 40 degrees C. The present study indicates that examination of dried feces on the wall can be an effective tool for surveillance of the natural infection of triatomine bugs that live in houses.
Hamano
S
S
Department of Parasitology, Faculty of Medical Sciences, Kyushu University, Maidashi 3-1-1, Higashi-ku, 812-8582, Fukuoka, Japan. hamano@parasite.med.kyushu-u.ac.jp
Horio
M
M
Miura
S
S
Higo
H
H
Iihoshi
N
N
Noda
K
K
Tada
I
I
Takeuchi
T
T
D000818:Animals; D001838:Bolivia; D017931:DNA Primers; D018105:DNA, Kinetoplast / Q000032:analysis*; D005243:Feces / Q000469:parasitology; D006798:Housing; D016133:Polymerase Chain Reaction; D013696:Temperature; D014224:Triatoma / Q000469:parasitology*; D014349:Trypanosoma cruzi / Q000235:genetics / Q000302:isolation & purification*
D017931:DNA Primers; D018105:DNA, Kinetoplast