title: Feline leukaemia provirus load during the course of experimental infection and in naturally infected cats.
abstract: Feline leukaemia virus (FeLV) infection in domestic cats can vary in its outcome (persistent, transient, no infection) for reasons that are not entirely known. It was hypothesized that the initial virus and provirus load could significantly influence the course of retrovirus infection. To determine the role of provirus loads, two methods of PCR, a nested PCR and a fluorogenic probe-based (TaqMan) real-time quantitative PCR, which were specific to the U3 region of FeLV-A were established. FeLV provirus in naturally and experimentally infected cats was then measured. Only 3 weeks after experimental FeLV-A infection, persistently infected cats demonstrated higher provirus loads and lower humoral immune responses than cats that had overcome antigenaemia. Lower initial provirus loads were associated with successful humoral immune responses. Unexpectedly, provirus in the buffy-coat cells of two cats that tested negative for the p27 antigen (a marker for viraemia) was also detected. In 597 Swiss cats, comparison of p27 antigen levels with PCR results revealed broad agreement. However, similar to the experimental situation, a significant number of animals (10%) was negative for the p27 antigen and FeLV-positive by PCR. These cats had a mean provirus load 300-fold lower than that of animals testing positive for the p27 antigen. In conclusion, an association between the provirus load and the outcome of FeLV infection was found. Detection of provirus carriers should contribute to further the control of FeLV. In addition, quantification of provirus loads will lead to a better understanding of FeLV pathogenesis and anti-retrovirus protective mechanisms.
authors: Clinical Laboratory, Department of Internal Veterinary Medicine, Faculty of Veterinary Medicine, University of Zurich, Winterthurerstrasse 260, CH-8057 Zurich, Switzerland1.
authors: Regina
authors: R
authors: Hofmann-Lehmann
authors: Clinical Laboratory, Department of Internal Veterinary Medicine, Faculty of Veterinary Medicine, University of Zurich, Winterthurerstrasse 260, CH-8057 Zurich, Switzerland1.
authors: Jon B
authors: JB
authors: Huder
authors: Clinical Laboratory, Department of Internal Veterinary Medicine, Faculty of Veterinary Medicine, University of Zurich, Winterthurerstrasse 260, CH-8057 Zurich, Switzerland1.
authors: Sabine
authors: S
authors: Gruber
authors: Clinical Laboratory, Department of Internal Veterinary Medicine, Faculty of Veterinary Medicine, University of Zurich, Winterthurerstrasse 260, CH-8057 Zurich, Switzerland1.
authors: Felicitas
authors: F
authors: Boretti
authors: Clinical Laboratory, Department of Internal Veterinary Medicine, Faculty of Veterinary Medicine, University of Zurich, Winterthurerstrasse 260, CH-8057 Zurich, Switzerland1.
authors: Brigitte
authors: B
authors: Sigrist
authors: Clinical Laboratory, Department of Internal Veterinary Medicine, Faculty of Veterinary Medicine, University of Zurich, Winterthurerstrasse 260, CH-8057 Zurich, Switzerland1.
authors: Hans
authors: H
authors: Lutz
mesh_terms: D000818:Animals; D002371:Cat Diseases / Q000821:virology*; D002415:Cats; D004195:Disease Models, Animal; D005256:Leukemia Virus, Feline / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D011533:Proviruses / Q000302:isolation & purification*; D012367:RNA, Viral / Q000302:isolation & purification; D012192:Retroviridae Infections / Q000662:veterinary*; D013047:Specific Pathogen-Free Organisms; D014412:Tumor Virus Infections / Q000662:veterinary*; D019562:Viral Load; D014766:Viremia
chemical_list: D018809:Proliferating Cell Nuclear Antigen; D012367:RNA, Viral; C413374:p27 antigen