Exposure of Caenorhabditis elegans to extremely low frequency high magnetic fields induces stress responses.
Responses of the small heat shock protein gene, hsp-16, were examined in transgenic Caenorhabditis elegans exposed to electromagnetic fields. Expression of the hsp-16-lacZ gene was enhanced when transgenic animals were exposed to magnetic fields up to 0.5 T at 60 Hz. The hsp-16 promoter was more efficiently expressed at the embryonic than at the post-embryonic stage irrespective of exposure. Promoter activity was more sensitive to the stimulus in the intestine at the post-embryonic stage. Evidence is presented that the induction occurs at the transcriptional step of hsp-16.
Miyakawa
T
T
Laboratory of Magnetic Fields Control and Applications, Faculty of Engineering, Kanazawa University, Ishikawa, Japan.
Yamada
S
S
Harada
S
S
Ishimori
T
T
Yamamoto
H
H
Hosono
R
R
D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D017173:Caenorhabditis elegans / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D029742:Caenorhabditis elegans Proteins; D017931:DNA Primers / Q000235:genetics; D015870:Gene Expression; D017238:Genes, Helminth; D006360:Heat-Shock Proteins / Q000235:genetics; D015801:Helminth Proteins / Q000235:genetics; D007763:Lac Operon; D008280:Magnetics / Q000009:adverse effects*; D011401:Promoter Regions, Genetic; D018510:RNA, Helminth / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013312:Stress, Physiological / Q000209:etiology / Q000235:genetics / Q000503:physiopathology
D029742:Caenorhabditis elegans Proteins; D017931:DNA Primers; D006360:Heat-Shock Proteins; D015801:Helminth Proteins; D018510:RNA, Helminth; D012333:RNA, Messenger