A protease-resistant prion protein isoform is present in urine of animals and humans affected with prion diseases.
Prion protein (PrP)(Sc), the only known component of the prion, is present mostly in the brains of animals and humans affected with prion diseases. We now show that a protease-resistant PrP isoform can also be detected in the urine of hamsters, cattle, and humans suffering from transmissible spongiform encephalopathies. Most important, this PrP isoform (UPrP(Sc)) was also found in the urine of hamsters inoculated with prions long before the appearance of clinical signs. Interestingly, intracerebrally inoculation of hamsters with UPrP(Sc) did not cause clinical signs of prion disease even after 270 days, suggesting it differs in its pathogenic properties from brain PrP(Sc). We propose that the detection of UPrP(Sc) can be used to diagnose humans and animals incubating prion diseases, as well as to increase our understanding on the metabolism of PrP(Sc) in vivo.
Shaked
G M
GM
Department of Neurology, the Agnes Ginges Center for Human Neurogenetics, Hadassah University Hospital, Jerusalem 91120, Israel.
Shaked
Y
Y
Kariv-Inbal
Z
Z
Halimi
M
M
Avraham
I
I
Gabizon
R
R
D000818:Animals; D002417:Cattle; D006224:Cricetinae; D010450:Endopeptidases / Q000378:metabolism*; D006801:Humans; D018620:PrPSc Proteins / Q000652:urine*; D017096:Prion Diseases / Q000175:diagnosis / Q000652:urine; D020033:Protein Isoforms / Q000652:urine*
D018620:PrPSc Proteins; D020033:Protein Isoforms; D010450:Endopeptidases