Analysis of IgG response to experimental infection with RH Toxoplasma gondii in goats.
The IgG response of goats experimentally infected with RH Toxoplasma gondii has been analysed using an indirect ELISA and Western-blot analysis. Specific IgG antibodies were first detected at 14 days post-inoculation (p.i.), reaching a peak by day 35 p.i. and showing slight fluctuations until the end of the experiment (91 p.i.). Specific IgG showed a reactivity over a whole range of peptides (125-24 kDa approximately), but the highest reactivity was observed against a group of antigens with a molecular weight between 34 and 28 kDa, in particular against a 30 kDa fraction which is considered to represent the major surface protein of T. gondii named p30 or SAG-1.
Conde
M
M
Departamento de Patología Animal (Parasitología y Enfermedades Parasitarias), Facultad de Veterinaria de la Universidad de Las Palmas de Gran Canaria, Spain.
Caballero
J M
JM
Rodríguez-Ponce
E
E
Ruiz
A
A
González
J
J
D000818:Animals; D000913:Antibodies, Protozoan / Q000096:biosynthesis* / Q000097:blood; D015153:Blotting, Western / Q000662:veterinary; D016054:DNA, Protozoan / Q000737:chemistry / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D015511:Goat Diseases / Q000276:immunology* / Q000469:parasitology; D006041:Goats; D006651:Histocytochemistry / Q000662:veterinary; D007074:Immunoglobulin G / Q000096:biosynthesis* / Q000097:blood; D016133:Polymerase Chain Reaction / Q000662:veterinary; D014122:Toxoplasma / Q000235:genetics / Q000276:immunology*; D014124:Toxoplasmosis, Animal / Q000097:blood / Q000276:immunology* / Q000469:parasitology
D000913:Antibodies, Protozoan; D016054:DNA, Protozoan; D007074:Immunoglobulin G