title: Overexpression of HER-2/neu enhances the sensitivity of human bladder cancer cells to urinary isoflavones.
abstract: Soybean foods have been suggested to be practical chemopreventives for human urinary tract cancers. Recently, we demonstrated that the co-operative action of isoflavones results in an increased dose-dependent growth inhibition and apoptosis than any single isoflavone compound. This study aimed to examine the potential of HER-2/neu as a biological target for soy isoflavones. The sensitivity of the bladder cancer cell lines (n=7) to the isoflavones was inversely related to the amount of HER-2/neu expressed. By using HER-2/neu transfection experiments, all three stable transfectants showed a significant growth inhibition by the isoflavone mixture at concentrations attainable in normal adult urine. An increased inhibition of tyrosine phosphorylation of proteins immunoprecipitated by HER-2/Neu was observed in the neu-transfectants compared with controls. The results of this study suggest that HER-2/neu may be a practical biochemical target for urinary isoflavones in vivo.
authors: Department of Medical Technology, Foo-Yin Institute of Technology, Kaohsiung, Republic of Taiwan, China.
authors: S
authors: S
authors: Su
authors: M
authors: M
authors: Lai
authors: T
authors: T
authors: Yeh
authors: N
authors: N
authors: Chow
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000627:therapeutic use*; D017209:Apoptosis / Q000502:physiology; D015153:Blotting, Western; D002455:Cell Division / Q000502:physiology; D015536:Down-Regulation; D015870:Gene Expression; D018734:Genes, erbB-2 / Q000235:genetics*; D006801:Humans; D007529:Isoflavones / Q000627:therapeutic use*; D019000:Phosphotyrosine / Q000378:metabolism; D008517:Phytotherapy; D013025:Glycine max; D014162:Transfection; D014407:Tumor Cells, Cultured; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000517:prevention & control
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D007529:Isoflavones; D019000:Phosphotyrosine