Identification of mutans streptococcal species by the PCR products of the dex genes.
A pair of polymerase chain reaction (PCR) primers was designed on the basis of the nucleotide sequence homology of dextranase genes (dex) of Streptococcus mutans, S. sobrinus and S. downei. The primer pair amplified a 530-bp DNA fragment on the dex genes of mutans streptococcal species: S. mutans, S. sobrinus, S. downei, S. rattus and S. cricetus. HaeIII digestion of the 530-bp fragments generated species-specific subfragments, which were easily distinguishable from each other by agarose gel electrophoresis. These results suggest that the PCR-amplification of the dex gene followed by the HaeIII digestion is useful for rapid identification of the five species of mutans streptococci.
Igarashi
T
T
Department of Oral Microbiology, Showa University School of Dentistry, 1-5-8 Hatanodai, Tokyo 142-8555, Shinagawa, Japan. igatakes@dent.showa-u.ac.jp
Ichikawa
K
K
Yamamoto
A
A
Goto
N
N
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D003910:Dextranase / Q000235:genetics*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D051381:Rats; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D013295:Streptococcus mutans / Q000201:enzymology / Q000235:genetics*
D017931:DNA Primers; D003910:Dextranase