Effects of inhalation of perfluorocarbon aerosol on oxygenation and pulmonary function compared to PGI2 inhalation in a sheep model of oleic acid-induced lung injury.
OBJECTIVE
To evaluate the effects of PFC aerosol compared to PGI2 aerosol and NaCl aerosol on gas exchange and lung mechanics in oleic acid-induced acute lung injury.


METHODS
A prospective, controlled, randomised, in vivo animal laboratory study.


METHODS
Research laboratory at an university hospital.


METHODS
Twenty one (n = 21) adult sheep of either gender weighing 26.8+/-6.4 kg.


METHODS
The animals were randomised to three groups: PFC aerosol (perfluorooctane), PFC group; prostacyclin aerosol (Flolan), PGI2 group; and NaCl aerosol (0.9% sodium chloride solution), control group. After induction of anaesthesia and placement of vascular catheters, lung injury was induced with 0.12 ml x kg(-1) oleic acid. Aerosols were continuously administered for 2 h using a jet nebuliser. Gas exchange, pulmonary mechanic, and haemodynamic parameters were obtained at regular intervals.


RESULTS
PFC aerosol increased oxygenation (PaO2) 15 min after the initiation of treatment up to 120 min (P < 0.05). Transpulmonary shunt improved in the PFC group (P < 0.05) while it did not change in the two other groups. PFC aerosol reduced maximum airway pressure (Pmax) (median) significantly from (median) 38 mbar to 32 mbar (P < 0.05). Static compliance improved significantly in the PFC group (P < 0.05).


CONCLUSIONS
The inhalation of a PFC aerosol led to a significant improvement in pulmonary mechanics and gas exchange, which was not observed in the other two groups. These data suggest that a small dose of perfluorocarbon will have beneficial effects on gas exchange and respiratory mechanics. Therefore, the non-invasive aerosol application technique seems to be a reasonable alternative to administer perfluorocarbons in severe lung injury.
Ragaller
M
M
Department of Anaesthesiology and Intensive Care Medicine, University Hospital Carl Gustav Carus, Medical Faculty, Technical University of Dresden, Germany. ragaller@rcs.urz.tu-dresden.de
Bleyl
J
J
Tschö
U
U
Winkler
T
T
Regner
M
M
Rasche
S
S
Koch
T
T
Albrecht
M
M
D000336:Aerosols / Q000008:administration & dosage; D000818:Animals; D004195:Disease Models, Animal; D016503:Drug Delivery Systems; D011464:Epoprostenol / Q000008:administration & dosage*; D005260:Female; D005466:Fluorocarbons / Q000008:administration & dosage*; D006439:Hemodynamics / Q000187:drug effects*; D008297:Male; D019301:Oleic Acid; D011175:Positive-Pressure Respiration; D011659:Pulmonary Gas Exchange / Q000187:drug effects*; D012128:Respiratory Distress Syndrome / Q000139:chemically induced / Q000503:physiopathology / Q000628:therapy*; D012756:Sheep
D000336:Aerosols; D005466:Fluorocarbons; D019301:Oleic Acid; C073608:perfluorooctane; D011464:Epoprostenol