title: Classical swine fever: morphological and morphometrical study of pulmonary intravascular macrophages.
abstract: To gain further insight into the pathogenesis of classical swine fever (CSF), the changes induced by hog cholera (HC) virus in pulmonary intravascular macrophages (PIMs) were examined. Twelve pigs were inoculated by the intramuscular route with a virulent strain of HC virus (Quillota strain) and killed in groups of three at 4, 7, 10 and 14 days post-inoculation. Immunohistochemical and ultrastructural examination revealed HC virus infection in endothelial cells, PIMs, and interstitial and alveolar macrophages. In addition to viral replication, a predominant feature was the secretory activation of PIMs, characterized by expanded rough endoplasmic reticulum and hyperplastic Golgi complexes. The results obtained suggest that macrophage activation and the subsequent release of pro-inflammatory mediators play an important role in the pathogenesis of CSF.
authors: Departamento de Anatomía y Anatomía Patológica Comparadas, Edificio de Sanidad Animal, Facultad de Veterinaria, Córdoba, 14014, Spain.
authors: L
authors: L
authors: Carrasco
authors: E
authors: E
authors: Ruiz-Villamor
authors: J C
authors: JC
authors: Gómez-Villamandos
authors: F J
authors: FJ
authors: Salguero
authors: M J
authors: MJ
authors: Bautista
authors: M
authors: M
authors: Maciá
authors: M
authors: M
authors: Quezada
authors: A
authors: A
authors: Jover
mesh_terms: D000818:Animals; D002452:Cell Count; D006691:Classical Swine Fever / Q000209:etiology / Q000473:pathology*; D006692:Classical Swine Fever Virus / Q000502:physiology* / Q000648:ultrastructure; D004730:Endothelium, Vascular / Q000473:pathology / Q000821:virology; D005260:Female; D007124:Immunoenzyme Techniques / Q000662:veterinary; D008168:Lung / Q000098:blood supply / Q000473:pathology* / Q000821:virology; D016676:Macrophages, Alveolar / Q000473:pathology* / Q000821:virology; D008297:Male; D008854:Microscopy, Electron / Q000662:veterinary; D015388:Organelles / Q000648:ultrastructure; D013552:Swine; D014759:Viral Envelope Proteins / Q000032:analysis
chemical_list: D014759:Viral Envelope Proteins; C087086:glycoprotein E2, classical swine fever virus