Proteins immunoreactive with antibody against a human leptin fragment are found in serum and tissues of the sea lamprey, Petromyzon marinus L.
An affinity-purified, polyclonal antibody raised against a peptide corresponding to amino acids 137-156 at the carboxy terminus of human leptin (16 kD) was used to search for immunoreactive protein(s) in the lamprey, Petromyzon marinus. Immunoblots of serum from different phases of the life cycle showed the presence of a 65-kD immunoreactive protein in the larvae and all stages of metamorphosis but not in feeding juvenile and upstream migrant adults. Extracts of tissues known to store fat were also examined using the same antibody. Muscle and fat column from all phases tested (larvae, stage 2 and 4 metamorphosing animals, feeding juveniles and upstream migrants) showed 100- and 50-kD immunoreactive proteins. Extracts of nephric fold, the primary site of fat storage during metamorphosis, lacked the 100-kD protein but had the 50 kD; they also had a 16 kD immunoreactive protein not found in the other tissues. The immunoreactivity of the proteins of both serum and tissue extracts was blocked by pretreatment of the antibody with the leptin-derived antigen. The results indicate that P. marinus has proteins that share at least one epitope with mammalian leptin.
Yaghoubian
S
S
Department of Zoology, Division of Life Sciences, University of Toronto at Scarborough, 1265 Military Trail, M1C 1A4, Toronto, ON, Canada.
Filosa
M F
MF
Youson
J H
JH
D000273:Adipose Tissue / Q000196:embryology / Q000378:metabolism; D000818:Animals; D000906:Antibodies / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes; D006801:Humans; D015151:Immunoblotting; D007798:Lampreys; D020738:Leptin / Q000737:chemistry* / Q000276:immunology*; D009132:Muscles / Q000196:embryology / Q000378:metabolism; D017434:Protein Structure, Tertiary; D013997:Time Factors
D000906:Antibodies; D000939:Epitopes; D020738:Leptin