Inhibition of calmodulin-dependent calcium-ATPase and phosphodiesterase by various cyclopeptides and peptide alkaloids from the Zizyphus species.
The effects of various sedative cyclopeptides and peptide alkaloids from the Zizyphus species on calmodulin-dependent Ca2+-ATPase and phosphodiesterase were investigated. Calmodulin-induced activation of Ca2+-ATPase was strongly inhibited by sanjoinine-A dialdehyde (IC0O, 2.3 microM), -Ah1 (IC50, 4.0 microM), -A (IC50, 4.6 microM), and -G2 (IC50, 7.2 microM), while calmodulin-induced activation of phosphodiesterase was strongly inhibited by both deachuine-S10 (IC30, 4.9 microM) and sanjoinine-D (IC50, 9.0 microM). The inhibitory activity of the various cyclopeptides and peptide alkaloids on Ca2+-ATPase was found to correlate well with their sedative activity.
Hwang
K H
KH
Natural Products Research Institute, Seoul National University, Korea.
Han
Y N
YN
Han
B H
BH
D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000037:antagonists & inhibitors*; D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors*; D000470:Alkaloids / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D000252:Calcium-Transporting ATPases / Q000037:antagonists & inhibitors; D002147:Calmodulin / Q000037:antagonists & inhibitors*; D054677:Cyclic Nucleotide Phosphodiesterases, Type 1; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D018482:Muscle, Skeletal / Q000201:enzymology; D010455:Peptides / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry*; D014176:Protein Biosynthesis; D011506:Proteins / Q000737:chemistry; D051381:Rats
D000470:Alkaloids; D002147:Calmodulin; D004791:Enzyme Inhibitors; D010455:Peptides; D011506:Proteins; D015105:3',5'-Cyclic-AMP Phosphodiesterases; D054677:Cyclic Nucleotide Phosphodiesterases, Type 1; D000251:Adenosine Triphosphatases; D000252:Calcium-Transporting ATPases