Cycloprodigiosin hydrochloride, a H+/Cl- symporter, induces apoptosis in human colon cancer cell lines in vitro.
Recently, we reported that cycloprodigiosin hydrochloride (cPrG.HCl), a novel H+/Cl- symporter, induces acidification of the cytosol and leads to apoptosis on rat and human liver cancer cells. In the present study, the effects of cPrG.HCl, a H+/Cl- symporter, were examined in colon cancer cell lines in vitro. In the MTT assay, cPrG.HCl inhibited the growth of two colon cancer cell lines (WiDr and SW480) in a dose- and time-dependent manner. The cPrG.HCl treatment of both types of cells induced apoptosis as confirmed by the appearance of a sub-G1 population and intranucleosomal DNA fragmentation. In addition, cPrG.HCl lowered pHi (below pH 6.8) respectively. Therefore, these results suggest that cPrG.HCl may be useful for the treatment of colon cancer cells.
Yamamoto
C
C
Department of Internal Medicine III, Kansai Medical University, Moriguchi, Osaka 570-8506, Japan. yamamotd@takii.kmu.ac.jp
Takemoto
H
H
Kuno
K
K
Yamamoto
D
D
Nakai
K
K
Baden
T
T
Kamata
K
K
Hirata
H
H
Watanabe
T
T
Inoue
K
K
D017920:Antiporters / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D003110:Colonic Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D004273:DNA, Neoplasm / Q000032:analysis; D005434:Flow Cytometry; D005562:Formazans; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D007211:Indoles / Q000494:pharmacology*; D011758:Pyrroles / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects; D013778:Tetrazolium Salts; D014407:Tumor Cells, Cultured / Q000187:drug effects* / Q000473:pathology
D017920:Antiporters; D004273:DNA, Neoplasm; D005562:Formazans; D007166:Immunosuppressive Agents; D007211:Indoles; D011758:Pyrroles; D013778:Tetrazolium Salts; C114260:hydrogen-chloride symporter; C079782:MTT formazan; C108328:cycloprodigiosin