Biosynthesis of fatty acids and triacylglycerols by 2,6,10,14-tetramethyl pentadecane-grown cells of Nocardia globerula 432.
Nocardia globerula strain 432 was able to synthesize triacylglycerols (TAG) during cultivation on 2,6,10,14-tetramethyl pentadecane (pristane) under nitrogen-limiting conditions. Within these cells, 4,8,12-trimethyl tridecanoic acid was the major fatty acid detected. Fatty acids with an odd number of carbon atoms and minor amounts of even-numbered fatty acids were also observed. Experiments carried out with acrylic acid, an inhibitor of beta-oxidation, suggested that odd-numbered fatty acids such as C15:0, C17:0 and 10-methyl C17:0 were synthesized de novo using propionyl-CoA, the beta-oxidation product, as precursor. Although N. globerula 432 incorporated mainly straight chain fatty acids into TAG, the branched fatty acid 4,8,12-trimethyl tridecanoic acid also appeared, to some extent, in the acylglycerols. The importance of TAG biosynthesis by pristane-grown cells of N. globerula strain 432 is discussed.
Alvarez
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CEIMA, Facultad de Ciencias Naturales, Universidad Nacional de la Patagonia SanJuan Bosco, Chubut, Argentina. halvarez@unpata.edu.ar
Souto
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Viale
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Pucci
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D000085:Acetates / Q000494:pharmacology; D000179:Acrylates / Q000494:pharmacology; D002273:Carcinogens / Q000494:pharmacology*; D004347:Drug Interactions; D005227:Fatty Acids / Q000096:biosynthesis / Q000737:chemistry*; D009615:Nocardia / Q000187:drug effects* / Q000378:metabolism; D013729:Terpenes / Q000494:pharmacology*; D014280:Triglycerides / Q000096:biosynthesis*
D000085:Acetates; D000179:Acrylates; D002273:Carcinogens; D005227:Fatty Acids; D013729:Terpenes; D014280:Triglycerides; C009042:pristane; C036658:acrylic acid