title: Stealth polycyanoacrylate nanoparticles as tumor necrosis factor-alpha carriers: pharmacokinetics and anti-tumor effects.
abstract: The objective of this study was to investigate the pharmacokinetics and in vivo anti-tumor effect of recombinant human tumor necrosis factor-alpha (rHuTNF-alpha) encapsulated in poly(methoxypolyethyleneglycol cyanoacrylate-co-n-hexadecyl cyanoacrylate) (PEG-PHDCA) nanoparticles. Our experimental results showed that PEG-PHDCA nanoparticles could extend the half-life of rHuTNF-alpha to 7.42 h and obviously change the protein biodistribution in tissues, and in particular, increase accumulation of rHuTNF-alpha in tumor. Compared with PHDCA nanoparticles and free rHuTNF-alpha, PEG-PHDCA nanoparticles loaded with rHuTNF-alpha showed higher antitumor potency at the same dose, which might be related to its higher accumulation in tumor tissues and longer plasma circulation time. Therefore, PEG-PHDCA nanoparticles could be an effective carrier for rHuTNF-alpha.
authors: Department of Pharmaceutics, School of Pharmacy, Fudan University, Shanghai, China. ypli@mail.shcnc.ac.cn
authors: Y P
authors: YP
authors: Li
authors: Y Y
authors: YY
authors: Pei
authors: Z H
authors: ZH
authors: Zhou
authors: X Y
authors: XY
authors: Zhang
authors: Z H
authors: ZH
authors: Gu
authors: J
authors: J
authors: Ding
authors: J J
authors: JJ
authors: Zhou
authors: X J
authors: XJ
authors: Gao
authors: J H
authors: JH
authors: Zhu
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000493:pharmacokinetics / Q000494:pharmacology; D003487:Cyanoacrylates / Q000008:administration & dosage*; D004337:Drug Carriers; D005260:Female; D006207:Half-Life; D006801:Humans; D051379:Mice; D011108:Polymers; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000008:administration & dosage* / Q000493:pharmacokinetics / Q000494:pharmacology
chemical_list: D000970:Antineoplastic Agents; D003487:Cyanoacrylates; D004337:Drug Carriers; D011108:Polymers; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha