Incorporation of alpha- and beta-LNA (locked nucleic acid) monomers in oligodeoxynucleotides with polarity reversals.
The thymidine monomers of LNA with both alpha- and beta-configuration are incorporated with polarity reversals (i.e., with 3'-3' and 5'-5' junctions) in oligodeoxynucleotides with beta- and alpha-configuration, respectively. A 5'-O-phosphoramidite of the beta-LNA monomer is synthesised. Large destabilisations of duplexes with both complementary DNA and RNA are observed for oligodeoxynucleotides containing the alpha-LNA monomer, whereas a duplex with complementary RNA of an alpha-oligodeoxynucleotide containing the beta-LNA monomer is not destabilised.
Christensen
N K
NK
Department of Chemistry, University of Southern Denmark, Odense University, DK-5230, Odense M, Denmark.
Dahl
B M
BM
Nielsen
P
P
D009696:Nucleic Acids / Q000737:chemistry*; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*
D009696:Nucleic Acids; D009838:Oligodeoxyribonucleotides