Cloning of full-length genomic DNA encoding human FcepsilonRI alpha-chain and its transcriptional regulation.
Two novel exons, named exon 1A and exon 2A, were found at 18.4 and 12.6 kb upstream from the exon known as the first exon of human FcepsilonRI alpha-chain gene. Transcription from the promoter present in the upstream of exon 1A was decreased by mutations introduced into the "first intron" between exon 1A and exon 2A, suggesting the presence of an intronic regulatory element in the intron. Consistent with this, electrophoretic mobility shift assay revealed the presence of a nuclear factor which bound the region in FcepsilonRI alpha-chain positive cells.
Nishiyama
C
C
Allergy Research Center, Juntendo University School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo, 113-8421, Japan. chinishi@med.juntendo.ac.jp
Hasegawa
M
M
Nishiyama
M
M
Takahashi
K
K
Yokota
T
T
Okumura
K
K
Ra
C
C
D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics; D005091:Exons; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D006801:Humans; D019169:Jurkat Cells; D008156:Luciferases / Q000235:genetics; D046911:Macromolecular Substances; D008407:Mast Cells; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D017455:Receptors, IgE / Q000235:genetics*; D015183:Restriction Mapping; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic; D014162:Transfection
D017931:DNA Primers; D018076:DNA, Complementary; D046911:Macromolecular Substances; D017455:Receptors, IgE; D008156:Luciferases