Genomic structure, chromosomal localization, and embryonic expression of the mouse homolog of PRCC, a gene associated with papillary renal cell carcinoma.
In a subset of papillary renal cell carcinomas a t(X;1)(p11;q21) chromosome translocation has repeatedly been reported. Positional cloning has demonstrated that, as a result of this translocation, the transcription factor TFE3 gene on the X-chromosome becomes fused to a novel gene, PRCC, on chromosome 1. Since as yet little is known about the function of PRCC, we sought to identify the mouse counterpart of the PRCC gene. Isolation and sequence analysis of a mouse Prcc cDNA revealed a high level of conservation between man and mouse, both at the nucleotide and protein level. As the human PRCC gene, the mouse Prcc gene is ubiquitously expressed. It shows low expression in all mouse fetal tissues examined. In addition, we identified a genomic cosmid clone containing the complete Prcc gene. The mouse Prcc gene consists of seven exons, all of which contain coding sequences. The small second exon, which was found to be located adjacent to the t(X;1) breakpoint in the human gene on chromosome 1, is also conserved between man and mouse. In mouse, Prcc is located on chromosome 3. These cDNA and genomic clones will be instrumental in the creation of mouse models for a further elucidation of the function of PRCC.
Weterman
M A
MA
Department of Human Genetics, University Medical Center, Nijmegen, The Netherlands. M.A.Weterman@amc.uva.nl
Wilbrink
M
M
Eleveld
M
M
Merkx
G
G
van Groningen
J J
JJ
van Rooijen
M
M
Geurts van Kessel
A
A
D000595:Amino Acid Sequence; D000818:Animals; D002291:Carcinoma, Papillary / Q000235:genetics*; D002292:Carcinoma, Renal Cell / Q000235:genetics*; D018797:Cell Cycle Proteins; D002875:Chromosomes / Q000235:genetics; D003001:Cloning, Molecular; D005091:Exons / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D017404:In Situ Hybridization, Fluorescence; D007438:Introns / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins; D020161:Physical Chromosome Mapping; D011506:Proteins / Q000737:chemistry / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D016415:Sequence Alignment
D018797:Cell Cycle Proteins; D009363:Neoplasm Proteins; C498808:Prcc protein, mouse; D011506:Proteins; D012333:RNA, Messenger