Determination of etoposide in human plasma and leukemic cells by high-performance liquid chromatography with electrochemical detection.
This paper describes a high-performance liquid chromatographic method with electrochemical detection for the determination of etoposide levels in plasma, total and non-protein bound concentration, and in leukemic cells. The precision for between-runs (n=6) was 7.0, 4.9, and 9.5%, the accuracy was 3.7, 7.1 and 6.3%, and within-runs precision (n=6) was 3.9, 2.9 and 5.1% for total plasma, non-protein bound plasma fraction and leukemic cells, respectively. The correlation coefficients (R2) were 1.00 for all calibration curves. These assays have been applied to analyze samples from one patient with acute myelogenous leukemia during 24 h after i.v. infusion of etoposide (100 mg/m2).
Zhou
R
R
Department of Oncology-Pathology at Radiumhemmet, Karolinska Institute and Hospital, Stockholm, Sweden. rong.zhou@cck.ki.se
Frostvik-Stolt
M
M
Liliemark
E
E
D000972:Antineoplastic Agents, Phytogenic / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004563:Electrochemistry; D005047:Etoposide / Q000097:blood*; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000097:blood*; D011786:Quality Control; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000972:Antineoplastic Agents, Phytogenic; D005047:Etoposide