Enhanced protein levels of protein thiol/disulphide oxidoreductases in placentae from pre-eclamptic subjects.
Recent studies have indicated that pre-eclampsia is closely associated with oxidative stress both in maternal circulation and in the placenta. Protein thiol/disulphide oxidoreductases, such as thioredoxin, glutaredoxin, and protein disulphide isomerase have recently been found to eliminate reactive oxygen species (ROS) and regenerate oxidatively damaged proteins. Protein thiol/disulphide oxidoreductases may also play a role in combating pre-eclampsia. In this study, we examined the accumulation of 4-hydroxy-2-nonenal (HNE)-modified proteins, which are markers of lipid peroxidation, in human placentae of normal and pre-eclamptic subjects. We also examined the protein levels of thioredoxin, glutaredoxin, and protein disulphide isomerase in placentae. Immunoblotting and immunohistochemistry showed that HNE-modified proteins accumulated to a greater extent in pre-eclamptic placentae than in normal placentae. In both normal and pre-eclamptic placentae, thioredoxin, glutaredoxin, and protein disulphide isomerase were detected in the trophoblasts of the floating villi. The levels of these proteins were increased approximately 2- to 3-fold in the pre-eclamptic placentae compared to the normal placentae. These results indicated that the pre-eclamptic placentae were exposed to oxidative stress and that the protein thiol/disulphide oxidoreductases were adaptively induced in pre-eclamptic placentae, suggesting possible roles for thioredoxin, glutaredoxin, and protein disulphide isomerase in protecting placental functions against oxidative stress caused by pre-eclampsia.
Shibata
E
E
Department of Obstetrics and Gynecology, University of Occupational and Environmental Health, Iseigaoka 1-1, Yahatanishi-ku, Kitakyushu 807-8555, Japan.
Ejima
K
K
Nanri
H
H
Toki
N
N
Koyama
C
C
Ikeda
M
M
Kashimura
M
M
D000328:Adult; D000447:Aldehydes / Q000378:metabolism; D005260:Female; D005865:Gestational Age; D054477:Glutaredoxins; D006801:Humans; D015151:Immunoblotting; D007150:Immunohistochemistry; D015227:Lipid Peroxidation; D018384:Oxidative Stress; D010088:Oxidoreductases; D010920:Placenta / Q000737:chemistry / Q000201:enzymology*; D011225:Pre-Eclampsia / Q000201:enzymology*; D011247:Pregnancy; D011490:Protein Disulfide Reductase (Glutathione) / Q000378:metabolism*; D019704:Protein Disulfide-Isomerases / Q000032:analysis / Q000378:metabolism; D011506:Proteins / Q000032:analysis / Q000378:metabolism; D013879:Thioredoxins / Q000032:analysis / Q000378:metabolism
D000447:Aldehydes; D054477:Glutaredoxins; D011506:Proteins; D013879:Thioredoxins; D010088:Oxidoreductases; D011490:Protein Disulfide Reductase (Glutathione); D019704:Protein Disulfide-Isomerases; C027576:4-hydroxy-2-nonenal