The transcription factors SOX9 and SOX10 are vitiligo autoantigens in autoimmune polyendocrine syndrome type I.
Vitiligo is common in the hereditary disorder autoimmune polyendocrine syndrome type I (APS I). Patients with APS I are known to have high titer autoantibodies directed against various tissue-specific antigens. Using sera from APS I patients for immunoscreening of a cDNA library from human scalp, we identified the transcription factors SOX9 and SOX10 as novel autoantigens related to this syndrome. Immunoreactivity against SOX9 was found in 14 (15%) and against SOX10 in 20 (22%) of the 91 APS I sera studied. All patients reacting with SOX9 displayed reactivity against SOX10, suggesting shared epitopes. Among the 19 patients with vitiligo, 12 (63%) were positive for SOX10 (p < 0.0001). Furthermore, three of 93 sera from patients with vitiligo unrelated to APS I showed strong reactivity against SOX10, which may indicate a more general role of SOX10 as an autoantigen in vitiligo.
Hedstrand
H
H
Department of Medical Sciences, University Hospital, S.E.-751 85, Uppsala, Sweden. hakan.hedstrand@medsci.uu.se
Ekwall
O
O
Olsson
M J
MJ
Landgren
E
E
Kemp
E H
EH
Weetman
A P
AP
Perheentupa
J
J
Husebye
E
E
Gustafsson
J
J
Betterle
C
C
Kämpe
O
O
Rorsman
F
F
D001324:Autoantigens / Q000276:immunology / Q000502:physiology*; D001483:Base Sequence; D015153:Blotting, Western; D017931:DNA Primers; D004268:DNA-Binding Proteins / Q000276:immunology / Q000502:physiology*; D005260:Female; D006609:High Mobility Group Proteins / Q000276:immunology / Q000502:physiology*; D006801:Humans; D008297:Male; D016884:Polyendocrinopathies, Autoimmune / Q000276:immunology*; D011233:Precipitin Tests; D011994:Recombinant Proteins / Q000276:immunology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055758:SOX9 Transcription Factor; D055757:SOXE Transcription Factors; D014157:Transcription Factors / Q000276:immunology / Q000502:physiology*; D014820:Vitiligo / Q000276:immunology*
D001324:Autoantigens; D017931:DNA Primers; D004268:DNA-Binding Proteins; D006609:High Mobility Group Proteins; D011994:Recombinant Proteins; C527923:SOX10 protein, human; D055758:SOX9 Transcription Factor; C527932:SOX9 protein, human; D055757:SOXE Transcription Factors; D014157:Transcription Factors