Model of interaction of the IL-1 receptor accessory protein IL-1RAcP with the IL-1beta/IL-1R(I) complex.
A preliminary model has been calculated for the activating interaction of the interleukin 1 receptor (IL-1R) accessory protein IL-1RAcP with the ligand/receptor complex IL-1beta/IL-1R(I). First, IL-1RAcP was modeled on the crystal structure of IL-1R(I) bound to IL-1beta. Then, the IL-1RAcP model was docked using specific programs to the crystal structure of the IL-1beta/IL-1R(I) complex. Two types of models were predicted, with comparable probability. Experimental data obtained with the use of IL-1beta peptides and antibodies, and with mutated IL-1beta proteins, support the BACK model, in which IL-1RAcP establishes contacts with the back of IL-1R(I) wrapped around IL-1beta.
Casadio
R
R
Laboratory of Biocomputing, University of Bologna, Italy. casadio@alma.unibo.it
Frigimelica
E
E
Bossù
P
P
Neumann
D
D
Martin
M U
MU
Tagliabue
A
A
Boraschi
D
D
D000818:Animals; D000906:Antibodies / Q000276:immunology; D000918:Antibody Specificity; D002460:Cell Line; D018360:Crystallography, X-Ray; D006801:Humans; D007375:Interleukin-1 / Q000737:chemistry* / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D053577:Interleukin-1 Receptor Accessory Protein; D051379:Mice; D008958:Models, Molecular; D009154:Mutation / Q000235:genetics; D011485:Protein Binding; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism*; D011817:Rabbits; D017472:Receptors, Interleukin-1 / Q000737:chemistry* / Q000378:metabolism*; D013601:T-Lymphocytes; D013950:Thymus Gland / Q000166:cytology
D000906:Antibodies; C507765:IL1RAP protein, human; C507767:Il1rap protein, mouse; D007375:Interleukin-1; D053577:Interleukin-1 Receptor Accessory Protein; D011506:Proteins; D017472:Receptors, Interleukin-1