[The expression of tPA directed by the bovine BLG regulatory elements in the mammary gland of transgenic mice].
In order to get the regulatory elements which are essential for generating mammary gland bioreactors, the whole 8.4 kb bovine BLG gene was obtained by PCR amplification. The 1.6 kb chicken lysozyme matrix attachment region (MAR) was used to overcome position effects. The bovine BLG-tPA expression vector was constructed and the BLG-tPA fusion gene was introduced into fertilized eggs of mice by microinjection to generate transgenic mouse. 170 offsprings were obtained, of which 9 were proved to be transgenic mice based on PCR and Southern-blot analysis. The tPA expression level amounted to 12 micrograms/mL in the milk of mice. The bovine BLG-tPA fusion gene integrated in the founders was inheritable.
Chen
H X
HX
Institute of Biotechnology, Academy of Military Medical Sciences, Beijing 100071, China.
Chen
X
X
Yang
X
X
Deng
J X
JX
Su
G F
GF
Huang
P T
PT
D000818:Animals; D002417:Cattle; D007782:Lactoglobulins / Q000235:genetics*; D008321:Mammary Glands, Animal / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis*; D010959:Tissue Plasminogen Activator / Q000235:genetics*
D007782:Lactoglobulins; D011993:Recombinant Fusion Proteins; D010959:Tissue Plasminogen Activator