title: In-vivo transfer of mecA DNA to Staphylococcus aureus [corrected].
abstract: Staphylococcus aureus is thought to have acquired mecA DNA by horizontal transfer. DNA fingerprints made by restriction nucleases that cut certain sequences of DNA are used to compare complete genomes or particular genes between bacteria. We isolated an epidemic mecA(-) meticillin-susceptible S aureus genotype and, subsequently, a rare isogeneic mecA(+) meticillin-resistant S aureus (MRSA) genotype from a neonate who had never been in contact with MRSA. This MRSA contained mecA DNA that was identical to that in a coagulase-negative staphylococcal strain isolated from this patient, but different from other MRSA genotypes. We believe that this MRSA was formed in vivo by horizontal transfer of the mecA DNA between two staphylococcal species.
authors: C L
authors: CL
authors: Wielders
authors: M R
authors: MR
authors: Vriens
authors: S
authors: S
authors: Brisse
authors: L A
authors: LA
authors: de Graaf-Miltenburg
authors: A
authors: A
authors: Troelstra
authors: A
authors: A
authors: Fleer
authors: F J
authors: FJ
authors: Schmitz
authors: J
authors: J
authors: Verhoef
authors: A C
authors: AC
authors: Fluit
mesh_terms: D016470:Bacteremia / Q000382:microbiology; D001426:Bacterial Proteins; D002352:Carrier Proteins / Q000235:genetics*; D003428:Cross Infection / Q000382:microbiology; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000235:genetics*; D022761:Gene Transfer, Horizontal / Q000235:genetics*; D005838:Genotype; D006602:Hexosyltransferases; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D008826:Microbial Sensitivity Tests; D002267:Muramoylpentapeptide Carboxypeptidase / Q000235:genetics*; D046915:Penicillin-Binding Proteins; D010458:Peptidyl Transferases; D013203:Staphylococcal Infections / Q000382:microbiology; D013211:Staphylococcus aureus / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D004269:DNA, Bacterial; D046915:Penicillin-Binding Proteins; D010458:Peptidyl Transferases; D006602:Hexosyltransferases; D002267:Muramoylpentapeptide Carboxypeptidase