title: Isolation and characterization of hepatic and intestinal expressed sequence tags potentially involved in trait differentiation between cows of different metabolic type.
abstract: mRNA differential display was applied to identify hepatic and intestinal expressed sequence tags (ESTs) in lactating cows of different metabolic types (milk type, meat/milk type, meat type) that are potentially associated with energy turnover and involved in the regulation of these processes. Altogether, 277 ESTs (liver: 161, intestine: 116) were identified. For 150 transcripts (liver: 99, intestine: 51), the sequences showed similarity to previously described genes and ESTs. Many of these homologous sequences are reported to be involved in hepatic metabolism. Ninety-four ESTs (liver: 43, intestine: 51) did not match with any database entries. Semi-quantitative RT-PCR revealed quantitative differences in transcript represented by randomly chosen ESTs in liver samples of animals of the Holstein and Charolais breeds. One hundred twenty-two ESTs were mapped physically by using a bovine-hamster somatic cell hybrid panel (SCP) and a 5000-rad bovine whole genome radiation hybrid panel (WGRH). These ESTs were assigned to the bovine syntenic groups and positioned in the recently established RH-based ordered comparative map of the cattle and human genomes. The mapped, differentially expressed sequence tags are a useful prerequisite for cloning of genetic variation underlying economic traits.
authors: Research Unit for Molecular Biology, Research Institute for the Biology of Farm Animals Dummerstorf, Wilhelm-Stahl-Allee 2, D-18196 Dummerstorf, Germany.
authors: U
authors: U
authors: Dorroch
authors: T
authors: T
authors: Goldammer
authors: R M
authors: RM
authors: Brunner
authors: S R
authors: SR
authors: Kata
authors: C
authors: C
authors: Kühn
authors: J E
authors: JE
authors: Womack
authors: M
authors: M
authors: Schwerin
mesh_terms: D000818:Animals; D002417:Cattle / Q000235:genetics*; D002874:Chromosome Mapping / Q000662:veterinary*; D004247:DNA / Q000737:chemistry / Q000235:genetics; D017931:DNA Primers; D020224:Expressed Sequence Tags; D005260:Female; D005819:Genetic Markers; D006822:Hybrid Cells / Q000528:radiation effects; D007413:Intestinal Mucosa / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008969:Molecular Sequence Data; D019655:Quantitative Trait, Heritable; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D005819:Genetic Markers; D012333:RNA, Messenger; D004247:DNA