Energy nutrients modulate the splanchnic sequestration of dietary nitrogen in humans: a compartmental analysis.
We used a previously developed compartmental model to assess the postprandial distribution and metabolism of dietary nitrogen (N) in the splanchnic and peripheral areas after the ingestion of a single meal containing milk protein either alone (MP) or with additional sucrose (SMP) or fat (FMP). The addition of fat was predicted to enhance splanchnic dietary N anabolism only transiently, without significantly affecting the global kinetics of splanchnic retention and peripheral uptake. In contrast, the addition of sucrose, which induced hyperinsulinemia, was predicted to enhance dietary N retention and anabolism in the splanchnic bed, thus leading to reduced peripheral dietary amino acid availability and anabolism. The incorporation of dietary N into splanchnic proteins was thus predicted to reach 18, 24, and 35% of ingested N 8 h after MP, FMP, and SMP, respectively. Such a model provides insight into the dynamics of the system in the nonsteady postprandial state and constitutes a useful, explanatory tool to determine the region-specific utilization of dietary N under different nutritional conditions.
Fouillet
H
H
Unité Mixte de Recherche de Physiologie de la Nutrition et du Comportement Alimentaire, Institut National de la Recherche Agronomique-Institut National Agronomique Paris-Grignon, F-75231 Paris, France. fouillet@inapg.inra.fr
Gaudichon
C
C
Mariotti
F
F
Bos
C
C
Huneau
J F
JF
Tomé
D
D
D000596:Amino Acids / Q000097:blood; D000641:Ammonia / Q000652:urine; D001692:Biological Transport / Q000187:drug effects / Q000502:physiology; D001825:Body Fluid Compartments / Q000502:physiology*; D004041:Dietary Fats / Q000378:metabolism* / Q000494:pharmacology; D019422:Dietary Sucrose / Q000378:metabolism* / Q000494:pharmacology; D016002:Discriminant Analysis; D005766:Gastrointestinal Contents / Q000737:chemistry; D006801:Humans; D007082:Ileum; D007328:Insulin / Q000097:blood; D007441:Intubation, Gastrointestinal; D007700:Kinetics; D008894:Milk Proteins / Q000378:metabolism; D008954:Models, Biological; D009584:Nitrogen / Q000378:metabolism*; D009587:Nitrogen Isotopes / Q000032:analysis; D019518:Postprandial Period / Q000502:physiology; D011237:Predictive Value of Tests; D015203:Reproducibility of Results; D013152:Splanchnic Circulation / Q000502:physiology*; D014508:Urea / Q000652:urine
D000596:Amino Acids; D004041:Dietary Fats; D019422:Dietary Sucrose; D007328:Insulin; D008894:Milk Proteins; D009587:Nitrogen Isotopes; D000641:Ammonia; D014508:Urea; D009584:Nitrogen