Use of real-time quantitative PCR to detect Chlamydophila felis infection.
A real-time PCR assay was developed to detect and quantify Chlamydophila felis infection of cats. The assay uses a molecular beacon to specifically identify the major outer membrane protein gene, is highly reproducible, and is able to detect fewer than 10 genomic copies.
Helps
C
C
Division of Molecular and Cellular Biology, Department of Clinical Veterinary Science, University of Bristol, Langford, Bristol, BS40 5DU, United Kingdom. c.r.helps@Bristol.ac.uk
Reeves
N
N
Tasker
S
S
Harbour
D
D
D000818:Animals; D002371:Cat Diseases / Q000175:diagnosis* / Q000382:microbiology; D002415:Cats; D023501:Chlamydophila / Q000235:genetics / Q000302:isolation & purification*; D023521:Chlamydophila Infections / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D003228:Conjunctiva / Q000382:microbiology; D003234:Conjunctivitis, Bacterial / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary; D004269:DNA, Bacterial / Q000032:analysis; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results
D004269:DNA, Bacterial