Continuous monitoring of arylesterase in human serum.
Arylesterase (EC 3.1.1.2) activity in serum was specifically measured using thiophenyl acetate in a mechanized assay at 37 degrees C with 4-bromophenylboronic acid as inhibitor of cholinesterase and hexacyanoferrate-III as indicator. The systematic development of a routine method, apparent limitations of thiophenyl compounds as arylesterase substrates, some kinetic constants of the enzyme, analytical variables such as precision (within-run <2% and between-run <2.5% relative standard deviation) and a preliminary reference interval (19.5-52.4 kU/l) for adults are described.
Lorentz
K
K
Institut für Klinische Chemie, Medizinische Universität zu Lübeck, Ratzeburger Allee 160, 23538 Lübeck, Germany.
Wirtz
W
W
Weiss
T
T
D000293:Adolescent; D002021:Buffers; D002265:Carboxylic Ester Hydrolases / Q000037:antagonists & inhibitors / Q000097:blood*; D004789:Enzyme Activation; D004791:Enzyme Inhibitors / Q000378:metabolism*; D005260:Female; D005292:Ferricyanides / Q000032:analysis*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008297:Male; D008875:Middle Aged; D008991:Monitoring, Physiologic / Q000379:methods; D016011:Normal Distribution; D010648:Phenylacetates / Q000737:chemistry / Q000378:metabolism*; D012016:Reference Values; D013379:Substrate Specificity; D013501:Surface-Active Agents / Q000737:chemistry
D002021:Buffers; D004791:Enzyme Inhibitors; D005292:Ferricyanides; D010648:Phenylacetates; D013501:Surface-Active Agents; C007931:hexacyanoferrate III; C012577:thiophenyl acetate; D002265:Carboxylic Ester Hydrolases; C019734:arylesterase