title: Unusual intracellular trafficking of Salmonella typhimurium in human melanoma cells.
abstract: Salmonella spp. are enterobacteria capable of invading and replicating in both professional and non-professional phagocytes. Here, we investigate the fate of S. typhimurium in human melanoma MelJuSo cells. The bacterium entered MelJuSo cells by a trigger mechanism and resided within a unique organelle, the Salmonella-containing vacuole (SCV). The SCV acquired early endosomal markers transiently and then underwent a series of membrane modifications. In HeLa cells, vacuole maturation is characterized by the simultaneous acquisition of the lysosomal membrane glycoproteins (Lgps) Lamp1, CD63 and vacuolar (v)-ATPase; in MelJuSo cells, however, acquisition of CD63 and v-ATPase preceded that of Lamp1. A very striking event in MelJuSo cells was the arrest of bacterial septation starting from 8 h after infection. Bacteria nevertheless continued to elongate, remained morphologically intact and viable and were eventually exocytosed. This original feature was observed in several skin-related cells including melanocytes, suggesting that it may provide the basis for an efficient host defence mechanism against Salmonella infection.
authors: Centre d'Immunologie de Marseille-Luminy, Case 906, 13288 Marseille Cedex 9, France.
authors: M J
authors: MJ
authors: Martínez-Lorenzo
authors: S
authors: S
authors: Méresse
authors: C
authors: C
authors: de Chastellier
authors: J P
authors: JP
authors: Gorvel
mesh_terms: D002455:Cell Division; D006367:HeLa Cells; D006801:Humans; D008546:Melanoma, Experimental / Q000382:microbiology*; D008954:Models, Biological; D010587:Phagocytosis; D012486:Salmonella typhimurium / Q000166:cytology / Q000472:pathogenicity*; D012867:Skin / Q000166:cytology / Q000382:microbiology*; D014617:Vacuoles / Q000382:microbiology