SwellGel: an affinity chromatography technology for high-capacity and high-throughput purification of recombinant-tagged proteins.
The revolution in genomics and proteomics is having a profound impact on drug discovery. Today's protein scientist demands a faster, easier, more reliable way to purify proteins. A high capacity, high-throughput new technology has been developed in Perbio Sciences for affinity protein purification. This technology utilizes selected chromatography media that are dehydrated to form uniform aggregates. The SwellGel aggregates will instantly rehydrate upon addition of the protein sample, allowing purification and direct performance of multiple assays in a variety of formats. SwellGel technology has greater stability and is easier to handle than standard wet chromatography resins. The microplate format of this technology provides high-capacity, high-throughput features, recovering milligram quantities of protein suitable for high-throughput screening or biophysical/structural studies. Data will be presented applying SwellGel technology to recombinant 6x His-tagged protein and glutathione-S-transferase (GST) fusion protein purification.
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Perbio Sciences, 2202 N. Bartlett Avenue, Milwaukee, Wisconsin, 53202-1009, USA.
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D001665:Binding Sites; D002614:Chelating Agents / Q000737:chemistry; D002846:Chromatography, Affinity / Q000295:instrumentation / Q000379:methods* / Q000639:trends; D003890:Desiccation; D015195:Drug Design; D005612:Freeze Drying; D005782:Gels; D005978:Glutathione / Q000737:chemistry; D005982:Glutathione Transferase / Q000737:chemistry / Q000302:isolation & purification; D006244:Hardness; D006639:Histidine / Q000737:chemistry; D008863:Microspheres; D009532:Nickel / Q000737:chemistry; D011993:Recombinant Fusion Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D014867:Water
D002614:Chelating Agents; D005782:Gels; D011993:Recombinant Fusion Proteins; D014867:Water; D006639:Histidine; D009532:Nickel; D005982:Glutathione Transferase; D005978:Glutathione