[Expression in Escherichia coli and immunogenicity of rotavirus VP7].
Rotaviruses are the single most important cause of severe diarrhea in young children all over the world. VP7 is the major outer capsid and is a primary candidate for inclusion in a subunit or recombinant vaccine. Part of the VP7 gene containing all the three antigenic regions was expressed as a chimeric protein with glutathione S-transferase (GST) in E. coli. The chimeric protein, representing about 30% of the total protein of the recombinant-plasmid-carrying bacteria, reacted with polyclonal antibodies raised against whole virus. Immunization of sero-negative rabbits and mice with purified fusion-protein generated both virus-binding and neutralizing antibodies.
Yuan
L Y
LY
Institute of Medical Biology, CAMS & PUMC, Kunming 650118, China. Liuyongsd@21cn.com
Liu
Y
Y
Li
C H
CH
Sun
M S
MS
Dai
C B
CB
D000818:Animals; D000956:Antigens, Viral; D015153:Blotting, Western; D002213:Capsid / Q000235:genetics* / Q000276:immunology; D036022:Capsid Proteins; D004926:Escherichia coli / Q000235:genetics*; D007114:Immunization; D051379:Mice; D016133:Polymerase Chain Reaction; D011817:Rabbits; D011994:Recombinant Proteins / Q000096:biosynthesis; D012401:Rotavirus / Q000276:immunology*
D000956:Antigens, Viral; D036022:Capsid Proteins; D011994:Recombinant Proteins; C050779:VP7 protein, Rotavirus