Differential expression of metallopanstimulin/S27 ribosomal protein in hepatic regeneration and neoplasia.
We have previously shown that human metallopanstimulin-1 (MPS-1) is a ubiquitous 9.4-kd multifunctional ribosomal S27/nuclear "zinc finger" protein that is expressed at high levels in a wide variety of actively proliferating cells and tumor tissues. In this study, we examined the expression of MPS-1 in chronic hepatitis, cirrhosis, and hepatocellular carcinoma. Tissue samples were obtained at the time of tumor resection, needle biopsy, or liver transplantation. MPS- 1 was studied by immunohistochemistry by use of specific antibodies to the N-terminus of MPS-1 in a biotin/streptavidin-amplified system. In chronic hepatitis, hepatocytes had very weak MPS-1 immunostaining. In contrast, hepatocytes in regenerating cirrhotic nodules stained strongly for MPS-1. In well-differentiated hepatocellular carcinoma, MPS-1 presence was intense at the periphery of the malignant nodule. In poorly differentiated hepatocellular carcinoma, MPS-1 presence was notably intense in malignant hepatocytes invading the septal tissues, in close contact with neovascular structures. These results suggest that MPS-1 may be involved in both progression toward malignancy in regenerating cirrhotic nodules and in subsequent steps of hepatocarcinogenesis.
Ganger
D R
DR
Section of Hepatology, Rush Presbyterian St Luke's Medical Center, Chicago, Illinois, USA.
Hamilton
P D
PD
Klos
D J
DJ
Jakate
S
S
McChesney
L
L
Fernandez-Pol
J A
JA
D000906:Antibodies / Q000032:analysis; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000473:pathology; D002454:Cell Differentiation; D002471:Cell Transformation, Neoplastic; D018450:Disease Progression; D015972:Gene Expression Regulation, Neoplastic; D006521:Hepatitis, Chronic / Q000235:genetics* / Q000473:pathology; D022781:Hepatocytes / Q000276:immunology / Q000502:physiology; D006801:Humans; D007150:Immunohistochemistry; D008103:Liver Cirrhosis / Q000235:genetics* / Q000473:pathology; D008113:Liver Neoplasms / Q000235:genetics* / Q000473:pathology; D008115:Liver Regeneration / Q000235:genetics / Q000502:physiology; D008667:Metalloproteins / Q000096:biosynthesis*; D009687:Nuclear Proteins / Q000096:biosynthesis*; D016601:RNA-Binding Proteins; D012269:Ribosomal Proteins / Q000096:biosynthesis*
D000906:Antibodies; D008667:Metalloproteins; D009687:Nuclear Proteins; D016601:RNA-Binding Proteins; C083686:RPS27 protein, human; D012269:Ribosomal Proteins; C096227:ribosomal protein S27a