title: Karyotypic analysis of the cotton boll weevil, Anthonomus grandis Boheman.
abstract: The diploid chromosome number of the cotton boll weevil, Anthonomus grandis Boheman, is 44. Both C- and N-banding techniques of mitotic cells demonstrated constitutive heterochromatin in the p arm of the eight largest chromosomes, the p arm of the X chromosome, and the centromeric region of autosomal groups A-D. Neither the y nor the group E autosomes appeared to contain constitutive heterochromatin. Supernumerary chromosomes were not found in the boll weevil. Restriction endonuclease banding of primary spermatocytes revealed a rod-shaped Xy tetrad in which the X and y were terminally associated. The p arm of the large, submetacentric X was C-band positive. While two of the autosomal tetrads were typically ring-shaped in primary spermatocytes, the remaining 19 autosomal tetrads were rod-shaped.
authors: Department of Biology, University of Memphis, TN 38152, USA.
authors: L R
authors: LR
authors: McNally
authors: M L
authors: ML
authors: Beck
authors: C J
authors: CJ
authors: Biggers
mesh_terms: D000818:Animals; D002875:Chromosomes; D001517:Coleoptera / Q000235:genetics*; D005260:Female; D017344:Genes, Insect; D007621:Karyotyping; D008297:Male