Postnatal disappearance of type A intercalated cells in carbonic anhydrase II-deficient mice.
Despite chronic acidosis, collecting ducts in adult carbonic anhydrase II-deficient (CAD mice) are depleted of intercalated cells, including those of type A, which are acid-secreting cells. We hypothesized that this depletion could occur during postnatal development. Principal cells were identified by immunofluorescence using an antibody to rat aquaporin-2 (AQP-2), and type A intercalated cells using an antibody specific for anion exchanger (AE1). In CAD mice the proportion of AQP2-positive cells, normal at 11 days, increased progressively in the cortical (CCD) and outer medullary collecting duct (OMCD), to reach almost 100% in the OMCD in adults. The percentage of AE1-positive cells in the OMCD of CAD mice decreased by half by 6 weeks of age and further by adulthood. In controls, however, the proportion of AQP2-positive cells and that of AE1-positive cells in the OMCD remained stable after 10 days of age. AE1-positive cells accounted for the majority of intercalated cells in the OMCD. The mechanisms leading to selective postnatal cell depletion in the collecting duct in CAD mice remain to be determined.
Brion
L P
LP
Albert Einstein College of Medicine and Montefiore Medical Center, Department of Pediatrics, Division of Neonatology and Pediatric Endocrinology, Weiler Hospital Room 725, 1825 Eastchester Road, Bronx, NY 10461, USA. brion@aecom.yu.edu
Suarez
C
C
Saenger
P
P
D000375:Aging / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn / Q000033:anatomy & histology* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D017920:Antiporters / Q000378:metabolism; D051399:Aquaporin 2; D051403:Aquaporin 6; D020346:Aquaporins / Q000378:metabolism; D002256:Carbonic Anhydrases / Q000172:deficiency*; D027963:Chloride-Bicarbonate Antiporters; D005455:Fluorescent Antibody Technique; D006579:Heterozygote; D007685:Kidney Tubules, Collecting / Q000378:metabolism / Q000473:pathology*; D008297:Male; D051379:Mice; D008856:Microscopy, Fluorescence
D017920:Antiporters; C496024:Aqp2 protein, mouse; D051399:Aquaporin 2; D051403:Aquaporin 6; D020346:Aquaporins; D027963:Chloride-Bicarbonate Antiporters; D002256:Carbonic Anhydrases