title: Measurement of homonuclear three-bond J(H(N)Halpha) coupling constants in unlabeled peptides complexed with labeled proteins: application to a decapeptide inhibitor bound to the proteinase domain of the NS3 protein of hepatitis C virus (HCV).
abstract: A new isotope-filtered experiment has been designed to measure homonuclear three-bond J(H(N)Halpha) coupling constants of unlabeled peptides complexed with labeled proteins. The new experiment is based on the 3D HNHA pulse scheme, and belongs to the 'quantitative J-correlation' type. It has been applied to a decapeptide inhibitor bound to the proteinase domain of the NS3 protein of human hepatitis C virus (HCV).
authors: IRBM P. Angeletti, Pomezia (Rome), Italy.
authors: D O
authors: DO
authors: Cicero
authors: G
authors: G
authors: Barbato
authors: U
authors: U
authors: Koch
authors: P
authors: P
authors: Ingallinella
authors: E
authors: E
authors: Bianchi
authors: S
authors: S
authors: Sambucini
authors: P
authors: P
authors: Neddermann
authors: R
authors: R
authors: De Francesco
authors: A
authors: A
authors: Pessi
authors: R
authors: R
authors: Bazzo
mesh_terms: D000465:Algorithms; D000998:Antiviral Agents / Q000737:chemistry* / Q000378:metabolism; D016174:Hepacivirus / Q000737:chemistry / Q000187:drug effects*; D046911:Macromolecular Substances; D019906:Nuclear Magnetic Resonance, Biomolecular; D009842:Oligopeptides / Q000737:chemistry* / Q000378:metabolism; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D011480:Protease Inhibitors / Q000737:chemistry* / Q000378:metabolism; D017434:Protein Structure, Tertiary; D017361:Viral Nonstructural Proteins / Q000737:chemistry* / Q000378:metabolism; D012697:Serine Endopeptidases; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases
chemical_list: D000998:Antiviral Agents; D046911:Macromolecular Substances; D009842:Oligopeptides; D010446:Peptide Fragments; D011480:Protease Inhibitors; D017361:Viral Nonstructural Proteins; C084422:NS3 protein, hepatitis C virus; D012697:Serine Endopeptidases; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases; C441458:P775 protein, synthetic