title: [Cloning and sequence analysis of truncated S gene from circulation of patients with chronic hepatitis B virus infection].
abstract: OBJECTIVE
  To find different mutated status of HBV DNA in circulation from chronic HBV patients.
  
  
  METHODS
  Specially designed primers and polymerase chain reaction method were applied to amplify the whole S gene of HBV from the serum of 2 patients. After being sequenced, 4 clones were compared with HBV adr subtype (China strain) to identify the mutant sites.
  
  
  RESULTS
  Sequencing results implied that there was a truncated large/middle S gene in the serum of the patients. Besides that, HBsAg and HBV DNA polymerase defective clones were also detected.
  
  
  CONCLUSIONS
  Truncated middle S gene is found in the circulation of patients with chronical HBV infection, suggestive of a poor prognosis.
authors: Gene Therapy Research Center, Institute of Infectious Diseases, 302nd Hospital of PLA, Beijing 100039, China.
authors: J
authors: J
authors: Dong
authors: J
authors: J
authors: Cheng
authors: Q
authors: Q
authors: Wang
authors: G
authors: G
authors: Wang
authors: S
authors: S
authors: Shi
authors: Y
authors: Y
authors: Liu
authors: X
authors: X
authors: Xia
authors: C
authors: C
authors: Si
mesh_terms: D003001:Cloning, Molecular; D004279:DNA, Viral / Q000097:blood*; D005260:Female; D005814:Genes, Viral; D006514:Hepatitis B Surface Antigens / Q000235:genetics*; D019694:Hepatitis B, Chronic / Q000097:blood / Q000821:virology*; D006801:Humans; D008875:Middle Aged; D011498:Protein Precursors / Q000235:genetics
chemical_list: D004279:DNA, Viral; D006514:Hepatitis B Surface Antigens; D011498:Protein Precursors; C050806:presurface protein 1, hepatitis B surface antigen; C050807:presurface protein 2, hepatitis B surface antigen