title: Quantitative measurement of thyroglobulin mRNA in peripheral blood of patients after total thyroidectomy.
abstract: Previous studies have reported the clinical usefulness of reverse transcription-polymerase chain reaction (RT-PCR) detection of thyroglobulin (TG) mRNA in the peripheral blood of patients with differentiated thyroid carcinoma. To evaluate this usefulness, we measured TG mRNA in the peripheral blood of patients diagnosed with thyroid carcinoma after total thyroidectomy by real-time quantitative RT-PCR using glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA as an internal control. Surprisingly, we detected TG mRNA in all samples obtained after total thyroidectomy, including those from 4 medullary carcinomas. Further, there was no statistical difference in expression levels of TG mRNA in the patients with or without metastasis, and no significant correlation was found between serum TG concentrations and the expression levels of TG mRNA. These results give rise to a question regarding the clinical applications of not only RT-PCR detection but also quantitative measurement of TG mRNA in peripheral blood.
authors: Department of Laboratory Medicine, Osaka University Medical School, D2 2-2 Yamadaoka, Suita, Osaka, 565-0871.
authors: T
authors: T
authors: Takano
authors: A
authors: A
authors: Miyauchi
authors: H
authors: H
authors: Yoshida
authors: Y
authors: Y
authors: Hasegawa
authors: K
authors: K
authors: Kuma
authors: N
authors: N
authors: Amino
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D005260:Female; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics; D006801:Humans; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000097:blood*; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013954:Thyroglobulin / Q000097:blood / Q000235:genetics*; D013964:Thyroid Neoplasms / Q000097:blood / Q000235:genetics* / Q000601:surgery; D013965:Thyroidectomy
chemical_list: D012333:RNA, Messenger; D013954:Thyroglobulin; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases