Suppression of apoptosis in hepatocytes by fructose-modified dendrimers.
By immobilizing fructose-modified dendrimers on a polystyrene culture plate, the number of initially adhered hepatocytes on it was increased. Moreover, increasing the number of generations of fructose-modified dendrimer (fructose-dendrimer) increased the number. Urea synthesis per unit area also was increased, corresponding to the increase in the number of initially adhered hepatocytes. This result suggests that the fructose-dendrimers do not cause a decline in cell function. On the other hand, apoptosis of hepatocytes occurs during cultivation, and results in a decrease in the number of adhered cells and a decline in cell function. Fructose-dendrimers were found to suppress apoptosis of hepatocytes. This characteristic is considered to be responsible for the increase in the number of initially adhered hepatocytes without a decline in cell function. Fructose-dendrimers are shown to be very suitable scaffolds for use in a high-performance bioartificial liver support system.
Kawase
M
M
Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Japan.
Shiomi
T
T
Matsui
H
H
Ouji
Y
Y
Higashiyama
S
S
Tsutsui
T
T
Yagi
K
K
D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology*; D002448:Cell Adhesion / Q000502:physiology; D018929:Cell Culture Techniques / Q000379:methods; D002455:Cell Division; D002478:Cells, Cultured; D005632:Fructose; D022781:Hepatocytes / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D008099:Liver / Q000166:cytology; D008297:Male; D011137:Polystyrenes; D051381:Rats; D017207:Rats, Sprague-Dawley
D001672:Biocompatible Materials; D011137:Polystyrenes; D005632:Fructose