Basis of the superiority of cefoperazone amphotericin teicoplanin for isolating Campylobacter upsaliensis from stools.
The optimum method for isolating Campylobacter upsaliensis from stools has not been clearly defined. In a preliminary study, cefoperazone amphotericin teicoplanin (CAT) selective medium isolated six C. upsaliensis strains which were not detected using modified cefoperazone charcoal deoxycholate (mCCDA). In order to identify the factors that underlie the superiority of CAT over mCCDA for isolating C. upsaliensis, we examined the effect of incubation time and antibiotic content of culture media on the growth of C. upsaliensis isolates using semiquantitative methods. The recovery of a subgroup of C. upsaliensis isolates from seeded stool specimens was also evaluated. Differences in growth of C. upsaliensis on CAT and mCCDA were modest and were not explained by the antibiotic profiles of the two media. Recovery of C. upsaliensis from spiked human feces on CAT was superior to that on mCCDA at lower concentrations of organisms (10(3) CFU/ml). We conclude that although CAT is more suitable than mCCDA for the isolation of C. upsaliensis from stools, the superiority of CAT for detecting this organism is not accounted for by the antibiotic composition of the medium.
Byrne
C
C
Children's Research Centre, Our Lady's Hospital for Sick Children, Dublin 12, Ireland.
Doherty
D
D
Mooney
A
A
Byrne
M
M
Woodward
D
D
Johnson
W
W
Rodgers
F
F
Bourke
B
B
D000666:Amphotericin B / Q000494:pharmacology; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002167:Campylobacter / Q000187:drug effects* / Q000254:growth & development / Q000302:isolation & purification*; D002169:Campylobacter Infections / Q000382:microbiology* / Q000662:veterinary; D002371:Cat Diseases / Q000382:microbiology; D002415:Cats; D002438:Cefoperazone / Q000494:pharmacology; D002648:Child; D003470:Culture Media / Q000737:chemistry; D004283:Dog Diseases / Q000382:microbiology; D004285:Dogs; D005243:Feces / Q000382:microbiology*; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods; D011817:Rabbits; D017334:Teicoplanin / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D003470:Culture Media; D017334:Teicoplanin; D002438:Cefoperazone; D000666:Amphotericin B