Allele-specific amplification for preimplantation genetic diagnosis (PGD) of spinal muscular atrophy.
We have developed a new allele-specific amplification method for the preimplantation genetic diagnosis (PGD) of spinal muscular atrophy (SMA; Werdnig-Hoffmann disease) from a single cell. This method is based on the detection of the deletion of exon 7 of the telomeric copy of the survival motor neurone (SMN(t)) gene. An oligonucleotide was designed to be specific to the SMN(t) nucleotidic sequence with exonic mismatch G (for SMN(t))-->A (for SMN(c)) at its 3' end. This test produces reliable PCR products in 95% of single lymphoblasts (85/88) tested as well as in 16/16 blastomeres from normal controls. Specificity analysis showed that we were able to detect homozygous deletion of the SMN(t) gene in 99% of single lymphoblasts (103/104) from a SMA patient. No contamination was detected in 68 blanks tested. Multiple cell and DNA dilution analysis revealed that the test is accurate and specific up to 100 pg DNA and should thus also be suitable for PGD at the blastocyst stage. This rapid procedure requires a single round of fluorescent PCR and no restriction digestion, while previously described single cell methods include nested PCR followed by restriction enzyme digestion. Two PGD cycles for SMA using this procedure were performed in our centre.
Moutou
C
C
Service de Biologie de la Reproduction-SIHCUS - CMCO, 19 rue Louis Pasteur, BP120, 67303 Schiltigheim cedex, France.
Gardes
N
N
Rongières
C
C
Ohl
J
J
Bettahar-Lebugle
K
K
Wittemer
C
C
Gerlinger
P
P
Viville
S
S
D000328:Adult; D000483:Alleles; D001757:Blastomeres; D017931:DNA Primers; D005260:Female; D005784:Gene Amplification; D006801:Humans; D008214:Lymphocytes; D008297:Male; D009134:Muscular Atrophy, Spinal / Q000175:diagnosis* / Q000235:genetics; D009154:Mutation / Q000235:genetics*; D021141:Nucleic Acid Amplification Techniques; D016133:Polymerase Chain Reaction / Q000379:methods*; D011247:Pregnancy; D019836:Preimplantation Diagnosis / Q000379:methods
D017931:DNA Primers