LytB, a novel gene of the 2-C-methyl-D-erythritol 4-phosphate pathway of isoprenoid biosynthesis in Escherichia coli.
The mevalonate-independent 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway for isoprenoid biosynthesis is essential in many eubacteria, plants, and the malaria parasite. Using genetically engineered Escherichia coli cells able to utilize exogenously provided mevalonate for isoprenoid biosynthesis by the mevalonate pathway we demonstrate that the lytB gene is involved in the trunk line of the MEP pathway. Cells deleted for the essential lytB gene were viable only if the medium was supplemented with mevalonate or the cells were complemented with an episomal copy of lytB.
Altincicek
B
B
Jomaa Pharmaka GmbH, Giessen, Germany. boran.altincicek@biochemie.med.uni-giessen.de
Kollas
A
A
Eberl
M
M
Wiesner
J
J
Sanderbrand
S
S
Hintz
M
M
Beck
E
E
Jomaa
H
H
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D004896:Erythritol / Q000031:analogs & derivatives / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029968:Escherichia coli Proteins; D017353:Gene Deletion; D005798:Genes, Bacterial / Q000235:genetics; D020043:Genes, Essential / Q000235:genetics; D005816:Genetic Complementation Test; D006801:Humans; D008798:Mevalonic Acid / Q000378:metabolism*; D010088:Oxidoreductases; D011106:Polyisoprenyl Phosphates / Q000096:biosynthesis* / Q000378:metabolism; D017385:Sequence Homology; D013403:Sugar Phosphates / Q000378:metabolism*
C114232:2-C-methylerythritol 4-phosphate; D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D011106:Polyisoprenyl Phosphates; D013403:Sugar Phosphates; D010088:Oxidoreductases; C109866:ispH protein, E coli; D004896:Erythritol; D008798:Mevalonic Acid