Identification and cloning of Lmairk, a member of the Aurora/Ipl1p protein kinase family, from the human protozoan parasite Leishmania.
Lmairk, a gene encoding a member of the Aurora/Ipl1p family of protein kinases (AIRK), was cloned from the protozoan parasite Leishmania major. Aurora kinases are key enzymes involved in the regulation of normal chromosome segregation during mitosis and cytokenesis of eukaryotic cells. This single-copy gene located on L. major chromosome 28 encodes a 301 amino acid polypeptide. All 11 conserved eukaryotic protein kinase catalytic subdomains are present and the proposed AIRK signature sequence was identified in the activation loop between subdomains VII and VIII. Lmairk is expressed, as an approximately 2.4 kb message, in at least three different species of Leishmania. This report represents the first identification of an AIRK from the trypanosomatid family of early divergent eukaryotes.
Siman-Tov
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Department of Parasitology, Hebrew University, Hadassah Medical School, Jerusalem, Israel.
Ivens
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Jaffe
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D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D020134:Catalytic Domain; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D016054:DNA, Protozoan; D018320:Leishmania major / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D011494:Protein Kinases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D017386:Sequence Homology, Amino Acid
D001426:Bacterial Proteins; D016054:DNA, Protozoan; D012333:RNA, Messenger; D011494:Protein Kinases; C432131:Airk protein, Leishmania mansoni