The interaction between Newcastle disease virus and Escherichia coli endotoxin in chickens.
The interaction between Newcastle disease virus (NDV) and Escherichia coli endotoxin was studied in cell cultures, embryonated chicken eggs, and 8-wk-old chickens. These interactions were evaluated according to the induction of specific or nonspecific resistance in the host system and the virus titer produced in both chicken embryos and chickens. The endotoxin of E. coli induced a decrease in the size of the bursa of Fabricius in live chickens. Escherichia coli endotoxin given intravenously induced plasma antiviral activity in chickens that was interpreted to be interferon, as detected in a vesicular stomatitis virus plaque reduction assay. Endotoxin failed to produced toxic effects in the chicken embryo fibroblasts (CEFs) or to result in any antiviral effect because no change was noted in the number of NDV plaques formed in CEF cultures. When endotoxin was given 3 days before NDV exposure in chickens, the virus titers were significantly (P < 0.05) decreased from a peak of 10(2) to 10(0.18), 10(2.5) to 10(0.18), and 10(2.5) to 0 in the spleens, lungs, and kidneys, respectively, at 72 hr post-NDV inoculation. When endotoxin was given 24 hr after NDV inoculation, the NDV titer significantly (P < 0.05) increased from 10(2.0) to 10(3.5), 10(2.5) to 10(6.5), 10(2.5) to 10(4.5), 0 to 10(2.5) in the spleen, lungs, kidneys, and liver, respectively, at 72 hr after NDV inoculation. In chicken sera, hemagglutination inhibition (HI) titer to NDV was significantly (P < 0.05) enhanced from 1164 to 3127 when endotoxin was given prior to virus inoculation. However, there was a decrease in HI to NDV from 1164 to 727 without a significant difference in chicken sera when NDV was given prior to endotoxin inoculation.
El Tayeb
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Department of Veterinary Preventive Medicine, The Ohio State University, Columbus 43210, USA.
Hanson
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D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D002060:Bursa of Fabricius / Q000187:drug effects* / Q000382:microbiology; D002478:Cells, Cultured; D002642:Chick Embryo; D002645:Chickens; D004731:Endotoxins / Q000008:administration & dosage / Q000494:pharmacology*; D004927:Escherichia coli Infections / Q000150:complications / Q000382:microbiology / Q000662:veterinary*; D005260:Female; D008297:Male; D009521:Newcastle Disease / Q000097:blood* / Q000150:complications; D009522:Newcastle disease virus / Q000187:drug effects / Q000472:pathogenicity / Q000502:physiology*; D011201:Poultry Diseases / Q000382:microbiology
D000914:Antibodies, Viral; D004731:Endotoxins; C044101:endotoxin, Escherichia coli