Reproductive efficiency and maternal-offspring transfer of gossypol in rainbow trout (Oncorhynchus mykiss) fed diets containing cottonseed meal.
In a preceding study, complete substitution of fish meal protein with cottonseed meal (CM) protein did not affect the survival or growth rate of adult rainbow trout over a 6-mo period. Gossypol, a naturally occurring compound in cottonseeds, has an antifertility effect in terrestrial animals, but information regarding salmonid fish is lacking. Female rainbow trout in this experiment were fed diets with either 0, 25, 50, 75, or 100% (diets 1 to 5) of the fish meal protein replaced with CM protein until first maturation and spawning to study long-term effects on growth and reproduction. Feeding diets containing CM over a total period of 10 mo did not result in differences in growth and mortality compared to the control group (P > 0.05). Increased CM incorporation levels resulted in decreased (P < 0.05) blood hemoglobin (10.6 +/- 1.3, 8.4 +/- 1.8, 7.3 +/- 1.1, 6.9 +/- 0.8, and 5.6 +/- 1.4 g/dL) and hematocrit (49.6 +/- 3.9, 38.5 +/- 9.3, 34.4 +/- 3.7, 34.8 +/- 4.9 and 28.0 +/- 6.8%) levels in diets 1 to 5, respectively. The CM incorporation level had no effect (P > 0.05) on the number of eggs produced per female but led to a reduction (P < 0.05) in egg weight. Eyed stage survival of embryos was low in all dietary groups and did not show differences (P > 0.05). However, an increasing CM incorporation level led to a linear increase (P < 0.05) in the number of females that produced no viable embryos (23.1, 37.5, 42.9, 60.0, and 71.4%). Gossypol in the diet was absorbed by the female trout and transferred to the eggs (0, 2.2 +/- 0.5, 6.7 +/- 1.6, 10.6 +/- 4.2, and 20.0 +/- 2.6 micrograms/g in diets 1 to 5, respectively). A high concentration of gossypol remained in the juveniles at the swim-up stage (endogenous yolk-absorbed) (0.6 +/- 0.3, 2.4 +/- 0.3, 3.4 +/- 0.0, and 4.7 +/- 1.0 micrograms/g, diets 2 to 5, respectively). The findings suggest that replacement of the dietary fish meal protein with CM protein has no effect on fish growth and mortality but may lead to a reduction in reproductive performance in female rainbow trout.
Blom
J H
JH
School of Natural Resources, Ohio State University, Columbus 43210, USA.
Lee
K J
KJ
Rinchard
J
J
Dabrowski
K
K
Ottobre
J
J
D000818:Animals; D003369:Cottonseed Oil / Q000378:metabolism / Q000494:pharmacology*; D004032:Diet / Q000662:veterinary*; D004044:Dietary Proteins / Q000378:metabolism / Q000494:pharmacology; D004958:Estradiol / Q000097:blood; D005260:Female; D006072:Gossypol / Q000378:metabolism / Q000494:pharmacology*; D006454:Hemoglobins / Q000032:analysis; D006908:Hydroxyprogesterones / Q000097:blood; D008431:Maternal-Fetal Exchange; D017686:Oncorhynchus mykiss / Q000254:growth & development*; D010060:Ovulation / Q000187:drug effects; D010063:Ovum / Q000187:drug effects; D011247:Pregnancy; D012098:Reproduction / Q000187:drug effects*; D013739:Testosterone / Q000097:blood
D003369:Cottonseed Oil; D004044:Dietary Proteins; D006454:Hemoglobins; D006908:Hydroxyprogesterones; C007433:17,20-dihydroxy-4-pregnen-3-one; D013739:Testosterone; D004958:Estradiol; D006072:Gossypol