Chromosome analysis and rDNA FISH in the stag beetle Dorcus parallelipipedus L. (Coleoptera: Scarabaeoidea: Lucanidae).
In the present work the chromosome complement (2n = 18; 8AA + XY) of the stag beetle Dorcus parallelipipedus L. (Scarabaeoidea: Lucanidae) is analyzed using conventional Giemsa staining, banding techniques and ribosomal fluorescent in situ hybridization (rDNA FISH). rDNA FISH remains the unique tool for providing a clear-cut identification of Nucleolar Organizer Regions (NORs) when conventional banding methods such as silver- and CMA3-staining proved to be inadequate. The dull, homogeneous CMA3 fluorescence of all chromosomes indicates the absence of markedly GC rich compartmentalized regions in D. parallelipipedus genome. Silver impregnation inadequacy in detecting NOR regions is to be sought in the unusual extensive silver stainability of heterochromatic material which, on the contrary of what stated for vertebrates, seems to be a common feature in Scarabaeoidea species.
Colomba
M S
MS
Dipartimento di Biologia Animale, Università di Palermo, Palermo, Italy.
Vitturi
R
R
Zunino
M
M
D000818:Animals; D002871:Chromosome Banding; D002875:Chromosomes / Q000648:ultrastructure*; D001517:Coleoptera / Q000235:genetics*; D004275:DNA, Ribosomal / Q000648:ultrastructure*; D005260:Female; D006570:Heterochromatin / Q000648:ultrastructure; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007621:Karyotyping; D008297:Male; D009697:Nucleolus Organizer Region / Q000648:ultrastructure; D016622:Silver Staining
D004275:DNA, Ribosomal; D006570:Heterochromatin