Multiplet component separation for measurement of methyl 13C-1H dipolar couplings in weakly aligned proteins.
A simple spectral editing procedure is described that generates separate subspectra for the methyl 13C-[1H3] multiplet components of 1H-13C HSQC spectra. The editing procedure relies on co-addition of in-phase and antiphase spectra and yields 1H-coupled constant-time HSQC subspectra for the methyl region that have the simplicity of the regular decoupled CT-HSQC spectrum. Resulting spectra permit rapid and reliable measurement of 1H-13C J and dipolar couplings. The editing procedure is illustrated for a Ca2+-calmodulin sample in isotropic and liquid crystalline phases.
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Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892-0520, USA.
Bax
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D000465:Algorithms; D000818:Animals; D002147:Calmodulin / Q000737:chemistry; D002244:Carbon / Q000737:chemistry; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D006859:Hydrogen / Q000737:chemistry; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D011487:Protein Conformation; D012680:Sensitivity and Specificity
D002147:Calmodulin; D002244:Carbon; D006859:Hydrogen