Identification of delta- and mu-type opioid receptors on human and murine dendritic cells.
The purpose of this study was to evaluate mu- and delta-opioid receptors (OR) on human and murine dendritic cells (DC). Expression of mu- and delta-OR mRNA on DC was demonstrated by RT-PCR. The immunocytochemical and Western blot analyses revealed the expression of OR protein in DC. Radioreceptor assay demonstrated the specific saturated temperature-dependent binding of [3H]-labeled opioid ligand on DC and B(max)=2.8+/-0.3 fmol/10(6) cells and K(D)=4.8+/-1.0 nM were calculated by a Scatchard analysis. Finally, OR ligands DADLE and DAGO dose-dependently modulated the capacity of DC to induce T cell proliferation in an MLR assay. Importantly, expression of functional OR on DC was significantly increased upon TNF-alpha-induced DC maturation. Thus, these data suggest a new mechanism of opioid-dependent neuroendocrine immunomodulation.
Makarenkova
V P
VP
National Research Center for Mental Health, 113152, Moscow, Russia.
Esche
C
C
Kost
N V
NV
Shurin
G V
GV
Rabin
B S
BS
Zozulya
A A
AA
Shurin
M R
MR
D000818:Animals; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000737:chemistry*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D012333:RNA, Messenger / Q000032:analysis; D017465:Receptors, Opioid, delta / Q000032:analysis* / Q000235:genetics / Q000502:physiology; D017450:Receptors, Opioid, mu / Q000032:analysis* / Q000235:genetics / Q000502:physiology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D012333:RNA, Messenger; D017465:Receptors, Opioid, delta; D017450:Receptors, Opioid, mu; D014409:Tumor Necrosis Factor-alpha