Conditional restoration of hippocampal synaptic potentiation in Glur-A-deficient mice.
Plasticity of mature hippocampal CA1 synapses is dependent on l-alpha-amino-3-hydroxy-5-methylisoxazole-4-propionate (AMPA) receptors containing the glutamate receptor A (GluR-A) subunit. In GluR-A-deficient mice, plasticity could be restored by controlled expression of green fluorescent protein (GFP)-tagged GluR-A, which contributes to channel formation and displayed the developmental redistribution of AMPA receptors in CA1 pyramidal neurons. Long-term potentiation (LTP) induced by pairing or tetanic stimulation was rescued in adult GluR-A(-/-) mice when (GFP)GluR-A expression was constitutive or induced in already fully developed pyramidal cells. This shows that GluR-A-independent forms of synaptic plasticity can mediate the establishment of mature hippocampal circuits that are prebuilt to express GluR-A-dependent LTP.
Mack
V
V
Department of Molecular Neurobiology, Max-Planck-Institute for Medical Research, D-69120 Heidelberg, Germany.
Burnashev
N
N
Kaiser
K M
KM
Rozov
A
A
Jensen
V
V
Hvalby
O
O
Seeburg
P H
PH
Sakmann
B
B
Sprengel
R
R
D000375:Aging; D000818:Animals; D003712:Dendrites / Q000378:metabolism; D004318:Doxycycline / Q000494:pharmacology; D004558:Electric Stimulation; D019706:Excitatory Postsynaptic Potentials; D049452:Green Fluorescent Proteins; D006624:Hippocampus / Q000378:metabolism / Q000502:physiology*; D017774:Long-Term Potentiation; D008164:Luminescent Proteins; D051379:Mice; D008822:Mice, Transgenic; D009473:Neuronal Plasticity; D018408:Patch-Clamp Techniques; D017966:Pyramidal Cells / Q000378:metabolism / Q000502:physiology*; D018091:Receptors, AMPA / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D013569:Synapses / Q000378:metabolism / Q000502:physiology*; D019076:Transgenes
D004318:Doxycycline; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins; D018091:Receptors, AMPA; D011993:Recombinant Fusion Proteins