title: Efficient synthesis of O-linked glycopeptide by a transglycosylation using endo alpha-N-acetylgalactosaminidase from Streptomyces sp.
abstract: Gal beta-(1-->3)-GalNAc-linked hexapeptide was synthesized by a transglycosylation using Gal beta-(1-->3)-GalNAc beta-pNP as a donor and a serine-containing hexapeptide as an acceptor using endo GalNAc-ase from Streptomyces sp.. The Gal beta-(1-->3)-GalNAc residue was transferred to the hydroxyl group of the serine residue of the peptide. The total yield of the glycopeptide via this process was better than that of the chemoenzymatic method. This process was confirmed to be a versatile method for the synthesis of O-linked glycopeptides.
authors: Nutrition Science Institute, Meiji Milk Products Co. Ltd., Odawara, Kanagawa, Japan. kajisaka@mbc.sphere.ne.jp
authors: K
authors: K
authors: Ajisaka
authors: M
authors: M
authors: Miyasato
authors: I
authors: I
authors: Ishii-Karakasa
mesh_terms: D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D006020:Glycopeptides / Q000096:biosynthesis* / Q000737:chemistry; D006031:Glycosylation; D006596:Hexosaminidases / Q000378:metabolism*; D019906:Nuclear Magnetic Resonance, Biomolecular; D048809:alpha-N-Acetylgalactosaminidase
chemical_list: D006020:Glycopeptides; D006596:Hexosaminidases; D048809:alpha-N-Acetylgalactosaminidase