Evidence for the presence of DNA-binding proteins involved in regulation of the gene expression of indole-3-pyruvic acid decarboxylase, a key enzyme in indole-3-acetic acid biosynthesis in Azospirillum lipoferum FS.
We isolated the ipdc gene coding for indole-3-pyruvic acid decarboxylase (IPDC), a key enzyme in the indole-3-pyruvic acid pathway for indole-3-acetic acid biosynthesis, in the plant growth-promoting rhizobacterium Azospirillum lipoferum FS. Gel mobility-shift assay showed the presence of two DNA-binding proteins that might be involved in regulation of the ipdc gene expression.
Yagi
K
K
Biotechnology Research Center, The University of Tokyo, Japan.
Chujo
T
T
Nojiri
H
H
Omori
T
T
Nishiyama
M
M
Yamane
H
H
D016949:Azospirillum / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D002262:Carboxy-Lyases / Q000235:genetics*; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins / Q000378:metabolism / Q000502:physiology*; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D007210:Indoleacetic Acids / Q000378:metabolism*; D008969:Molecular Sequence Data
D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; D007210:Indoleacetic Acids; C030737:indoleacetic acid; C068832:indolepyruvate decarboxylase; D002262:Carboxy-Lyases