Physical mapping of CSF2RA, ANT3 and STS on the pseudoautosomal region of bovine chromosome X.
The pseudoautosomal region (PAR) of bovine chromosome X (BTA X) has a particularly low representation of genes and markers, making comparative gene mapping in this region difficult. We describe the localization of three genes, colony-stimulating factor 2 receptor alpha (CSF2RA), ADP/ATP translocase 3 (ANT3) and steroid sulphatase (STS) on PAR of BTA X using a 5000 rad whole-genome radiation hybrid panel. The relationship of these genes to a number of previously mapped simple sequence repeat (microsatellite) markers is determined by physical and radiation hybrid mapping methods. The resulting radiation hybrid map resolves a discrepancy between the two major bovine linkage maps in the PAR of BTA X.
Moore
S S
SS
Department of Agriculture Food and Nutritional Science, University of Alberta, 4-10 Forestry/Agriculture Centre, University of Alberta T6G 2P5, Edmonton, Alberta, Canada. smoore@afns.ualberta.ca
Byrne
K
K
Johnson
S E
SE
Kata
S
S
Womack
J E
JE
D000818:Animals; D001192:Arylsulfatases / Q000235:genetics*; D002417:Cattle / Q000235:genetics*; D005260:Female; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics; D000226:Mitochondrial ADP, ATP Translocases / Q000235:genetics*; D021101:Radiation Hybrid Mapping; D016184:Receptors, Colony-Stimulating Factor / Q000235:genetics*; D043266:Steryl-Sulfatase; D014960:X Chromosome / Q000235:genetics*
D016184:Receptors, Colony-Stimulating Factor; D000226:Mitochondrial ADP, ATP Translocases; D001192:Arylsulfatases; D043266:Steryl-Sulfatase