Inhibition of human gelatinases by metals released from dental amalgam.
The interaction between metal ions and the oral environment is a major subject matter in dental research. Matrix metalloproteinases (MMPs) have been implicated in pathologic oral processes such as periodontal tissue destruction, root caries, tumor invasion and temporomandibular joint disorders. The aim of this study was to test the effect of metal ions released from dental amalgam on the major gingival gelatinolytic MMPs. Gingival human explants were cultured overnight in DMEM and the activity of secreted enzymes was analyzed by gelatin zymography in buffers conditioned with dispersed phase or concentional phase dental amalgams. The major enzymes present in conditioned media were characterized as MMP-2 and MMP-9 by immunoprecipitation. The proteolytic activities of MMP-2 and MMP-9 were strongly inhibited by dispersed phase amalgams conditioned buffers. Inhibition of MMP-2 and MMP-9 activities was partly prevented by the addition of 1,10 phenanthroline, a divalent metal chelator, to the amalgam conditioned buffers. Dental amalgam conditioned buffer also inhibited the degradation of denatured type I collagen by purified MMP-2 on liquid phase assays. These findings suggest that the activity of oral tissue MMPs may be modulated by metal ions released from dental amalgam.
Souza
A P
AP
Department de Morfologia, Faculdade de Odontologia de Piracicaba, UNICAMP, Piracicaba, SP, Brazil.
Gerlach
R F
RF
Line
S R
SR
D000328:Adult; D002413:Cations, Divalent / Q000494:pharmacology; D017077:Culture Media, Conditioned; D003723:Dental Amalgam / Q000737:chemistry*; D004492:Edetic Acid / Q000494:pharmacology; D005033:Ethylmaleimide / Q000494:pharmacology; D005260:Female; D018093:Gelatinases / Q000037:antagonists & inhibitors*; D005881:Gingiva / Q000201:enzymology; D006801:Humans; D008297:Male; D061965:Matrix Metalloproteinase Inhibitors; D008875:Middle Aged; D010618:Phenanthrolines / Q000494:pharmacology; D010664:Phenylmethylsulfonyl Fluoride / Q000494:pharmacology; D011480:Protease Inhibitors / Q000494:pharmacology*; D015032:Zinc / Q000494:pharmacology*
D002413:Cations, Divalent; D017077:Culture Media, Conditioned; D061965:Matrix Metalloproteinase Inhibitors; D010618:Phenanthrolines; D011480:Protease Inhibitors; D010664:Phenylmethylsulfonyl Fluoride; D003723:Dental Amalgam; D004492:Edetic Acid; D018093:Gelatinases; D015032:Zinc; D005033:Ethylmaleimide; C025205:1,10-phenanthroline