Vitamin E increases natural cytotoxic activity in seabream (Sparus aurata L.).
The natural cytotoxic activity of head-kidney leucocytes from gilthead seabream (Sparus aurata L.), after in vitro and in vivo vitamin E treatment, against tumor cells was studied by flow cytometry. Leucocytes were incubated in culture medium with different vitamin E supplementations (0.01-10 microg ml(-1)) for 6, 24 or 48 h and the results demonstrate that all the assayed vitamin E supplementations significantly enhanced the natural cytotoxic activity of leucocytes. To determine the effect of a high dietary level of vitamin E on this activity, fish were fed with 0 (control), 600, 1200 or 1800 mg of vitamin E supplementation kg(-1) diet for 2, 4 or 6 weeks. After 2 and 4 weeks of treatment, the natural cytotoxic activity was significantly enhanced at the highest (1.8 g kg(-1) diet) and lowest (600 mg kg(-1) diet) vitamin E supplement dosage, respectively. No effect of the vitamin E supplemented diet on seabream leucocyte natural cytotoxic cell activity was observed after 6 weeks of treatment.
Cuesta
A
A
Department of Cell Biology, Faculty of Biology, University of Murcia, Spain.
Esteban
M A
MA
Ortuño
J
J
Meseguer
J
J
D000818:Animals; D003601:Cytotoxicity Tests, Immunologic / Q000662:veterinary; D003602:Cytotoxicity, Immunologic / Q000187:drug effects*; D004032:Diet; D005434:Flow Cytometry / Q000662:veterinary; D007962:Leukocytes / Q000187:drug effects* / Q000276:immunology*; D051379:Mice; D011897:Random Allocation; D021541:Sea Bream / Q000276:immunology*; D014407:Tumor Cells, Cultured / Q000187:drug effects; D014810:Vitamin E / Q000008:administration & dosage / Q000494:pharmacology*
D014810:Vitamin E