Detection of abundantly transcribed genes and gene translocation in human immunodeficiency virus-associated non-Hodgkin's lymphoma.
Several novel, differentially transcribed genes were identified in one centroblastic and one immunoblastic HIV-associated B-cell non-Hodgkin's lymphoma (B-NHL) by subtractive cloning. In both lymphomas, we detected an upregulated transcription of several mitochondrial genes. In the centroblastic B-NHL, we found a high level transcription of nuclear genes including the interferon-inducible gene (INF-ind), the immunoglobulin light chain gene (IgL), the set oncogene, and several unknown genes. The data obtained on upregulated expression of the genes in human B-NHL of HIV-infected patients considerably overlap with those obtained earlier for the B-NHL of simian immunodeficiency virus-infected monkeys. In the centroblastic lymphoma, one transcript revealed a fusion of the 3'-untranslated region of the set gene and the C-terminal region of the IgL gene. This chimeric sequence was confirmed by a site-directed polymerase chain reaction performed with total cDNA and genomic DNA. The expected amplification product was obtained in both cases pointing to a genomic rearrangement. The IgL-set fusion sequence was not found in cDNA preparations and genomic DNA of the immunoblastic HIV-associated B-NHL. Further studies are necessary to determine whether these genes contribute to lymphoma development or can be used as therapeutic targets.
Tarantul
V
V
Department of Viral and Cellular Molecular Genetics, Institute of Molecular Genetics, Moscow 123182, Russia. tarantul@img.ras.ru
Nikolaev
A
A
Hannig
H
H
Kalmyrzaev
B
B
Muchoyan
I
I
Maximov
V
V
Nenasheva
V
V
Dubovaya
V
V
Hunsmann
G
G
Bodemer
W
W
D020413:3' Untranslated Regions; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism; D016208:Databases, Factual; D004305:Dose-Response Relationship, Drug; D006801:Humans; D015151:Immunoblotting; D007136:Immunoglobulins / Q000378:metabolism; D008223:Lymphoma / Q000378:metabolism; D016483:Lymphoma, AIDS-Related / Q000378:metabolism*; D008228:Lymphoma, Non-Hodgkin / Q000821:virology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism*; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic; D015854:Up-Regulation
D020413:3' Untranslated Regions; D018076:DNA, Complementary; D007136:Immunoglobulins; D012333:RNA, Messenger