Antiallergic action of Magnolia officinalis on immediate hypersensitivity reaction.
We studied the effect of aqueous extract of Magnolia officinalis bark (Magnoliaceae) (MOAE) on the immediate hypersensitivity reaction. MOAE (0.01 to 1 g/kg) dose-dependently inhibited compound 48/80 induced systemic anaphylaxis in rats. MOAE (0.1 and 1 g/kg) also significantly inhibited local immunoglobulin E (IgE)-mediated passive cutaneous anaphylactic reaction. When MOAE was pretreated at concentrations ranging from 0.01 to 1 g/kg, the levels of plasma histamine were reduced in a dose-dependent manner. MOAE (0.001 to 1 mg/ml) dose-dependently inhibited the histamine release from rat peritoneal mast cells (RPMC) activated by compound 48/80 or anti-dinitrophenyl (DNP) IgE. The level of cyclic AMP (cAMP) in RPMC, when MOAE was added, significantly increased compared with that of the normal control. Moreover, MOAE (0.01 to 1 mg/ml) had a significant inhibitory effect on anti-DNP IgE-induced tumor necrosis factor-alpha production from RPMC. These results indicate that MOAE inhibits immediate hypersensitivity reaction in vivo and in vitro.
Shin
T Y
TY
College of Pharmacy, Woosuk University, Chonju, Chonbuk, Korea. tyshin@core.woosuk.ac.kr
Kim
D K
DK
Chae
B S
BS
Lee
E J
EJ
D000818:Animals; D018926:Anti-Allergic Agents / Q000627:therapeutic use*; D000242:Cyclic AMP / Q000096:biosynthesis; D006636:Histamine Release / Q000187:drug effects; D006969:Hypersensitivity, Immediate / Q000188:drug therapy*; D007202:Indicators and Reagents; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism; D010323:Passive Cutaneous Anaphylaxis / Q000187:drug effects; D008517:Phytotherapy; D010936:Plant Extracts / Q000627:therapeutic use*; D010946:Plants, Medicinal / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis; D003189:p-Methoxy-N-methylphenethylamine / Q000037:antagonists & inhibitors / Q000494:pharmacology
D018926:Anti-Allergic Agents; D007202:Indicators and Reagents; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D003189:p-Methoxy-N-methylphenethylamine; D000242:Cyclic AMP