Streptomyces matensis laminaripentaose hydrolase is an 'inverting' beta-1,3-glucanase.
The laminaripentaose-producing beta-1,3-glucanase of Streptomyces matensis is a member of the glycoside hydrolase family GH-64. We have constructed and purified a recombinant hexahistidine-tagged form of the enzyme for characterisation. The enzyme, which exists as a monomer in solution, hydrolyses beta-1,3-glucan by a mechanism leading to overall inversion of the anomeric configuration. This is the first determination of the mechanism prevailing in glycoside hydrolase family GH-64 and this is the first characterisation of an 'inverting' beta-1,3-glucanase.
Nishimura
T
T
Architecture et Fontion des Macromolécules Biologiques, CNRS, Universités d'Aix-Marseille I and II, Marseille, France.
Bignon
C
C
Allouch
J
J
Czjzek
M
M
Darbon
H
H
Watanabe
T
T
Henrissat
B
B
D001704:Biopolymers / Q000737:chemistry / Q000378:metabolism; D002850:Chromatography, Gel; D004926:Escherichia coli / Q000235:genetics; D043326:Glucan 1,3-beta-Glucosidase; D005936:Glucans / Q000737:chemistry / Q000378:metabolism; D006639:Histidine; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D010455:Peptides / Q000235:genetics / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D020836:Protein Structure, Quaternary; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D013237:Stereoisomerism; D013302:Streptomyces / Q000201:enzymology* / Q000235:genetics; D047071:beta-Glucans; D001617:beta-Glucosidase / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*
D001704:Biopolymers; D005936:Glucans; D010455:Peptides; D011134:Polysaccharides; D011993:Recombinant Fusion Proteins; D047071:beta-Glucans; C033223:polyhistidine; D006639:Histidine; C008247:laminaran; C033363:beta-1,3-glucan; D001617:beta-Glucosidase; D043326:Glucan 1,3-beta-Glucosidase