title: Immunohistochemistry of the liver and biliary tree in extrahepatic biliary atresia.
abstract: BACKGROUND
  Progressive destruction of intrahepatic bile ducts may determine outcome in extrahepatic biliary atresia (EHBA) despite successful portoenterostomy. The aim of this study was to characterize the inflammatory infiltrate of a large series of cases of biliary atresia and relate these findings to clinical outcome.
  
  
  METHODS
  Immunohistochemical analysis was performed on frozen tissue sections of extrahepatic biliary tree and liver biopsies obtained (August 1996 to March 1998) from 28 infants with EHBA and 8 liver biopsy specimens from age-matched controls with other cholestatic liver disorders. A semiquantitative scoring system was designed to evaluate the staining with a panel of antibodies to the CD4, CD8, CD25, CD56, CD68, CD71 antigens and to HLA-DR, ICAM-1, VCAM-1, E-selectin and LFA-1. The infants then underwent followup prospectively and divided into 2 prognostic groups at 12 months postoperatively: those who had cleared their jaundice (graded as a good outcome [n = 19]), and those who required liver transplantation or who had failed to clear their jaundice (defined as > 50 micromol/L; graded as poor outcome [n = 9]).
  
  
  RESULTS
  CD4(+) lymphocytes and CD56(+) (NK cells) predominated in the liver of infants with EHBA as compared with controls. The infiltrating cells exhibited marked proliferation (CD71 expression) and activation (particularly LFA-1 but also CD25 expression). A smaller subpopulation of the cells also expressed VCAM and E-selectin. HLA-DR was strongly expressed on Kupffer cells and to a lesser extent on proliferating bile ducts and sinusoidal endothelium. Expression of the majority of markers was lower in the remnant bile duct tissue than in the liver of EHBA (P <.05) with only HLA-DR and LFA-1 (on infiltrating cells) and ICAM (on endothelium) expressed strongly in the remnant bile duct tissue. Although quantitatively less pronounced, all of these immunohistochemical features also were noted in non-EHBA cholestatic liver tissue. A good outcome at 12 months was associated with lower CD68 (macrophage) expression in both the liver (P <.05) and biliary tree (P <.05) and with reduced expression of ICAM-1 (P =.05) on infiltrating cells in the biliary remnant.
  
  
  CONCLUSIONS
  Immunohistochemical patterns of immune-mediated liver injury and inflammation were prevalent features at the time of portoenterostomy. They were neither exclusive to nor characteristic of EHBA. A reduction in the expression of the macrophage marker (CD68) within the liver and biliary remnants and reduction of ICAM-1 expression on infiltrating cells in the biliary remnants appear to be associated with a better postoperative prognosis.
authors: Department of Paediatric Surgery, Child Health, Institute of Liver Studies, King's College Hospital, Denmark Hill, London, England, UK.
authors: M
authors: M
authors: Davenport
authors: C
authors: C
authors: Gonde
authors: R
authors: R
authors: Redkar
authors: G
authors: G
authors: Koukoulis
authors: M
authors: M
authors: Tredger
authors: G
authors: G
authors: Mieli-Vergani
authors: B
authors: B
authors: Portmann
authors: E R
authors: ER
authors: Howard
mesh_terms: D015703:Antigens, CD / Q000032:analysis; D000944:Antigens, Differentiation, B-Lymphocyte / Q000032:analysis; D015214:Antigens, Differentiation, Myelomonocytic / Q000032:analysis; D017734:Bile Ducts, Extrahepatic / Q000737:chemistry / Q000473:pathology*; D001656:Biliary Atresia / Q000378:metabolism / Q000473:pathology* / Q000601:surgery; D016516:CD4-CD8 Ratio; D019040:E-Selectin / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D007223:Infant; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis; D007694:Killer Cells, Natural / Q000473:pathology; D008099:Liver / Q000737:chemistry / Q000473:pathology*; D016169:Lymphocyte Function-Associated Antigen-1 / Q000032:analysis; D008264:Macrophages / Q000473:pathology; D011171:Portoenterostomy, Hepatic; D011379:Prognosis; D015375:Receptors, Interleukin-2 / Q000032:analysis; D011990:Receptors, Transferrin; D019010:Vascular Cell Adhesion Molecule-1 / Q000032:analysis; D000097382:CD68 Molecule
chemical_list: D015703:Antigens, CD; D000944:Antigens, Differentiation, B-Lymphocyte; D015214:Antigens, Differentiation, Myelomonocytic; D019040:E-Selectin; D018799:Intercellular Adhesion Molecule-1; D016169:Lymphocyte Function-Associated Antigen-1; D015375:Receptors, Interleukin-2; D011990:Receptors, Transferrin; D019010:Vascular Cell Adhesion Molecule-1; C067980:CD68 antigen, human; D000097382:CD68 Molecule; C068126:CD71 antigen