title: The adventitia of atherosclerotic coronary arteries frequently contains Chlamydia pneumoniae.
abstract: The presence of Chlamydia pneumoniae in the human arterial system has mainly been determined in atherosclerotic plaque, whereas the adventitia has remained relatively unexplored. We assessed the presence of C. pneumoniae in all three vessel wall layers of coronary (n=72) and brachial (n=48) arteries in relation to local atherosclerosis. Immunohistochemical staining of C. pneumoniae was observed in plaque and adventitia. Cells stained for C. pneumoniae were detected in the same areas as cells stained for macrophages in adjacent sections. C. pneumoniae staining in the adventitia was associated with the extent and severity of atherosclerosis. Coronary sections with C. pneumoniae staining in both adventitia and plaque more often contained advanced atherosclerosis than sections with staining only in the adventitia. Staining was observed more often in the coronary artery than in the brachial artery (24/72 vs. 5/48 and 51/72 vs. 8/48 for plaque and adventitia, respectively, P=0.004 and P<0.001). PCR confirmed the presence of C. pneumoniae DNA in the adventitia. In summary, the adventitia of atherosclerotic coronary arteries frequently contains C. pneumoniae that seems to be located within macrophages. These results might indicate a possible route for infected circulating macrophages to home into atherosclerotic lesions in the artery via vasa vasorum.
authors: Department of Cardiology, Room G02-523, University Medical Center Utrecht, Heidelberglaan 100, 3584 CX Utrecht, The Netherlands.
authors: A
authors: A
authors: Vink
authors: G
authors: G
authors: Pasterkamp
authors: M
authors: M
authors: Poppen
authors: A H
authors: AH
authors: Schoneveld
authors: D P
authors: DP
authors: de Kleijn
authors: P J
authors: PJ
authors: Roholl
authors: J
authors: J
authors: Fontijn
authors: S
authors: S
authors: Plomp
authors: C
authors: C
authors: Borst
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D023521:Chlamydophila Infections / Q000150:complications / Q000473:pathology; D016993:Chlamydophila pneumoniae / Q000302:isolation & purification*; D003324:Coronary Artery Disease / Q000209:etiology / Q000382:microbiology* / Q000473:pathology; D005260:Female; D006801:Humans; D008297:Male; D017539:Tunica Intima / Q000382:microbiology / Q000473:pathology; D017540:Tunica Media / Q000382:microbiology / Q000473:pathology