A novel plant alpha4-fucosyltransferase (Vaccinium myrtillus L.) synthesises the Lewis(a) adhesion determinant.
We have partially characterised an alpha4-fucosyltransferase (alpha4-FucT) from Vaccinium myrtillus, which catalysed the biosynthesis of the Lewis(a) adhesion determinant. The enzyme was stable up to 50 degrees C. The optimum pH was 7.0, both in the presence and in the absence of Mn(2+). The enzyme was inhibited by Mn(2+) and Co(2+), and showed resistance towards inhibition with N-ethylmaleimide. It transferred fucose to N-acetylglucosamine in the type I Galbeta3GlcNAc motif from oligosaccharides linked to a hydrophobic tail and glycoproteins (containing the type I motif). Sialylated oligosaccharides containing the type II Galbeta4GlcNAc motif were not acceptors. The catalytic mechanism of the plant alpha4-FucT possibly involves a His residue, and it must have arisen by convergent evolution relative to its mammalian counterparts.
Palma
A S
AS
Instituto de Tecnologia Quimica e Biológica, Oeiras, Portugal.
Vila-Verde
C
C
Pires
A S
AS
Fevereiro
P S
PS
Costa
J
J
D002448:Cell Adhesion / Q000502:physiology; D005647:Fucosyltransferases / Q000302:isolation & purification / Q000378:metabolism*; D007983:Lewis Blood Group Antigens / Q000096:biosynthesis*; D019684:Magnoliopsida / Q000201:enzymology* / Q000378:metabolism; D013379:Substrate Specificity
D007983:Lewis Blood Group Antigens; D005647:Fucosyltransferases