Restriction enzyme BstZ17I is sensitive to cytosine methylation.
The cleavage patterns of a subset of restriction enzymes are blocked or impaired when a methylated CpG is overlapped with either the 5' or 3' end of the canonical restriction site. BstZ17I restriction endonuclease is a blunt-end cutter, which recognises the hexanucleotide sequence GTA(downward arrow)TAC. In this report, I show that the BstZ17I restriction enzyme is sensitive to cytosine methylation. Using both in vitro-methylated episomal plasmids and lambdaDNA, I demonstrate that the BstZ17I restriction enzyme is sensitive to cytosine methylation that occurs 3' and/or 5' of the canonical recognition sequence.
Kanduri
C
C
Department of Animal Development and Genetics, Norbyvägen 18A, s-75236, Evolutionary Biology Centrum, Uppsala University, Uppsala, Sweden. kanduri.chandrasekhar@ebc.uu.se
D018899:CpG Islands / Q000502:physiology; D003596:Cytosine / Q000378:metabolism*; D019175:DNA Methylation; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics; D013379:Substrate Specificity
D003596:Cytosine; D004262:DNA Restriction Enzymes