Isolation and sequence analysis of the gene encoding triose phosphate isomerase from Zygosaccharomyces bailii.
The ZbTPI1 gene encoding triose phosphate isomerase (TIM) was cloned from a Zygosaccharomyces bailii genomic library by complementation of the Saccharomyces cerevisiae tpi1 mutant strain. The nucleotide sequence of a 1.5 kb fragment showed an open reading frame (ORF) of 746 bp, encoding a protein of 248 amino acid residues. The deduced amino acid sequence shares a high degree of homology with TIMs from other yeast species, including some highly conserved regions. The analysis of the promoter sequence of the ZbTPI1 revealed the presence of putative motifs known to have regulatory functions in S. cerevisiae. The GenBank Accession No. of ZbTPI1 is AF325852.
Merico
A
A
Dipartimento di Fisiologia e Biochimica Generali, Sezione Biochimica Comparata, Università degli Studi di Milano, Piazza della Scienza 2, 20126 Milan, Italy.
Rodrigues
F
F
Côrte-Real
M
M
Porro
D
D
Ranzi
B M
BM
Compagno
C
C
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D005800:Genes, Fungal / Q000235:genetics; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D012441:Saccharomyces cerevisiae / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014305:Triose-Phosphate Isomerase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D020068:Zygosaccharomyces / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development
D014305:Triose-Phosphate Isomerase