Determination of carbohydrates as their p-sulfophenylhydrazones by capillary zone electrophoresis.
p-Hydrazinobenzenesulfonic acid was explored as an ultraviolet labeling reagent for capillary electrophoresis of mono-, di- and trisaccharides. The labeling reaction that produces p-sulfophenylhydrazines took less than 8 min, and introduced both chromphore and charged groups into the carbohydrate molecules. The derivatives of nine mono- and disaccharides were completely separated in 9 min using a 100 mM borate buffer at pH 10.24. On-column UV detection at 200 nm allowed the detection of glucose with a mass detection limit of 17.6 fmol or a concentration limit of 3.6 microM. Reproducible quantification of carbohydrates was achieved in the concentration range of 0.1-9.1 mM in reaction solution. The method was applied successfully to determine the monosaccharide composition of laminaran.
Wang
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Center for Molecular Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing, People's Republic of China.
Chen
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Y
D000117:Acetylglucosamine / Q000032:analysis; D001089:Arabinose / Q000032:analysis; D001565:Benzoates / Q000737:chemistry*; D004396:Coloring Agents / Q000737:chemistry*; D019075:Electrophoresis, Capillary; D005936:Glucans; D006601:Hexoses / Q000032:analysis; D006835:Hydrazones / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D008320:Maltose / Q000032:analysis; D011134:Polysaccharides / Q000032:analysis; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D014312:Trisaccharides / Q000032:analysis* / Q000737:chemistry
D001565:Benzoates; D004396:Coloring Agents; D005936:Glucans; D006601:Hexoses; D006835:Hydrazones; D011134:Polysaccharides; D014312:Trisaccharides; D008320:Maltose; C008247:laminaran; C053198:4-hydrazinobenzoic acid; D001089:Arabinose; D000117:Acetylglucosamine