title: Frog oocytes: a living test tube for studies on metabolic regulation.
abstract: This review is intended to illustrate how live frog oocytes may be advantageously used to address the study of some problems of in vivo glucose metabolism. Glucose microinjected into the cells is preferentially committed to glycogen synthesis. We present evidence showing that both the direct and indirect pathways for polysaccharide deposition are operative in oocytes. A small amount of the injected glucose (<5%) is released as labeled CO2 mainly through the pentose-P pathway. Coinjection of NADP+ and glucose significantly stimulates 14CO2 production, half-maximal stimulation being obtained at 0.13 mM. Finally, we show the use of frog oocytes to measure in vivo the control coefficient of hexokinase on glycogen synthesis and the pentose-P pathway. A value of 0.7 was found for the control coefficient of hexokinase on glycogen synthesis, while the enzyme has no control at all over the pentose-P pathway. Therefore, the frog oocyte may be used as a living test tube for the study of almost any metabolic process of interest.
authors: Departamento de Biología, Facultad de Ciencias, Universidad de Chile, Santiago. tiureta@uchile.cl
authors: T
authors: T
authors: Ureta
authors: A
authors: A
authors: Preller
authors: E
authors: E
authors: Kessi
mesh_terms: D000818:Animals; D001001:Anura; D002022:Bufo arenarum; D002245:Carbon Dioxide / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006003:Glycogen / Q000096:biosynthesis; D006593:Hexokinase / Q000378:metabolism; D008954:Models, Biological; D009249:NADP / Q000378:metabolism; D009865:Oocytes / Q000378:metabolism*; D010427:Pentose Phosphate Pathway; D011894:Rana pipiens; D011898:Ranidae; D014982:Xenopus laevis
chemical_list: D002245:Carbon Dioxide; D009249:NADP; D006003:Glycogen; D006593:Hexokinase; D005947:Glucose