title: The diagnostic value of crude or boiled extracts to identify tolerant versus nontolerant lentil-sensitive children.
abstract: OBJECTIVE
  The aim of this study was to compare two types of lentil extracts for use in skin prick tests for the diagnosis of lentil clinical allergy.
  
  
  METHODS
  Thirty-six patients with a history of allergic reactions after the ingestion of lentils were skin tested with two types of lentil extracts at 0.05, 0.5, 5, and 10 mg/mL. Both extracts were extracted at 40 degrees C and afterward, one of them was boiled for 15 minutes. Thirty-three of these patients underwent oral challenges with lentils and three had a convincing recent history of lentil anaphylaxis.
  
  
  RESULTS
  Twenty patients had a positive oral challenge; 13 were negative. Skin prick tests performed with the boiled extract at 0.5 and 5 mg/mL were positive in 96% and 100% of patients with positive food challenge, and in 31% and 85% of those with negative food challenge, respectively; positive skin test results were similar in both groups using the crude extract. Mean wheal sizes using the boiled extract at 0.5, 5, and 10 mg/mL were significantly greater in patients with a positive oral challenge than in those with a negative one (4.9, 6.8, and 7.4 mm versus 1.9, 3.5, and 5.1 mm, respectively; P < 0.05) These mean values were not statistically different using the crude extract.
  
  
  CONCLUSIONS
  These data suggest that lentil extracts for the diagnosis of lentil hypersensitivity should be heated, since boiled extracts, used at a concentration of 0.5 or 5 mg/mL, best identify clinically sensitive individuals.
authors: Allergy Department, Hospital Niño Jesús, Madrid, Spain.
authors: M
authors: M
authors: Martínez San Ireneo
authors: M D
authors: MD
authors: Ibáñez Sandín
authors: E
authors: E
authors: Fernández-Caldas
authors: F
authors: F
authors: Marañón
authors: M C
authors: MC
authors: Muñoz Martínez
authors: M T
authors: MT
authors: Laso Borrego
mesh_terms: D000284:Administration, Oral; D000293:Adolescent; D000485:Allergens / Q000009:adverse effects; D002648:Child; D002675:Child, Preschool; D007887:Fabaceae / Q000009:adverse effects*; D005260:Female; D005512:Food Hypersensitivity / Q000175:diagnosis* / Q000209:etiology; D006358:Hot Temperature; D006801:Humans; D007108:Immune Tolerance; D007223:Infant; D008297:Male; D010936:Plant Extracts / Q000008:administration & dosage / Q000276:immunology*; D010946:Plants, Medicinal; D012882:Skin Tests
chemical_list: D000485:Allergens; D010936:Plant Extracts