Sensitive ribonuclease protection assay employing glycogen as a carrier and a single inactivation/precipitation step.
Harju
S
S
University of Kansas Medical Center, 3901 Rainbow Boulevard, Kansas City, KS 66160-7421, USA.
Peterson
K R
KR
D000818:Animals; D020029:Base Pairing; D002021:Buffers; D011232:Chemical Precipitation; D018244:Chromosomes, Artificial, Yeast / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D005821:Genetic Techniques; D005914:Globins / Q000235:genetics; D006003:Glycogen / Q000737:chemistry*; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization; D015347:RNA Probes; D016372:RNA, Antisense; D012330:RNA, Double-Stranded / Q000302:isolation & purification; D012333:RNA, Messenger / Q000032:analysis* / Q000235:genetics; D006163:Ribonuclease T1 / Q000037:antagonists & inhibitors / Q000378:metabolism*; D012680:Sensitivity and Specificity; D013379:Substrate Specificity
D002021:Buffers; D015347:RNA Probes; D016372:RNA, Antisense; D012330:RNA, Double-Stranded; D012333:RNA, Messenger; D005914:Globins; D006003:Glycogen; D006163:Ribonuclease T1