title: Secretion of the novel Trichinella protein TSJ5 by T. spiralis and T. pseudospiralis muscle larvae.
abstract: The Trichinella tsJ5 gene is preferentially expressed in muscle larvae of Trichinella spiralis and encodes a novel protein. Previous observations have shown tsJ5 to be expressed at higher levels in encapsulating species than in non-encapsulating species and down-regulation of gene expression in T. pseudospiralis to be correlated with a lower protein abundance in the muscle larva of this species. In the present study we have determined the full-length cDNA sequence of the tsJ5 homologue in T. pseudospiralis (tpJ5). Antigens recognised by an anti-J5 antibody are found on the cuticular surface of both T. spiralis and T. pseudospiralis muscle larvae, as well as in the body wall muscle. We show that both the TSJ5 and TPJ5 proteins are found in the excretory/secretory fractions collected from muscle larva cultured in vitro and that despite the absence of a typical N-terminal signal sequence, secretion of pTSJ5 is mediated through the classical ER/Golgi secretory pathway.
authors: Institute of Parasitology, University of Bern, CH-3012, Bern, Switzerland.
authors: S
authors: S
authors: Kuratli
authors: A
authors: A
authors: Hemphill
authors: J
authors: J
authors: Lindh
authors: D F
authors: DF
authors: Smith
authors: B
authors: B
authors: Connolly
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D018076:DNA, Complementary; D018509:DNA, Helminth / Q000032:analysis / Q000235:genetics; D005455:Fluorescent Antibody Technique / Q000379:methods; D015801:Helminth Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D007814:Larva / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D008969:Molecular Sequence Data; D009124:Muscle Proteins; D018482:Muscle, Skeletal / Q000469:parasitology*; D012097:Repressor Proteins / Q000737:chemistry / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D014234:Trichinella / Q000254:growth & development / Q000378:metabolism*; D017160:Trichinella spiralis / Q000254:growth & development / Q000378:metabolism; D014235:Trichinellosis / Q000469:parasitology
chemical_list: D018076:DNA, Complementary; D018509:DNA, Helminth; D015801:Helminth Proteins; C435910:J5 protein, Trichinella pseudospiralis; D009124:Muscle Proteins; D012097:Repressor Proteins; C112551:tsJ5 protein, Trichinella spiralis