Method for recording spinal reflexes in mice: effects of thyrotropin-releasing hormone, DOI, tolperisone and baclofen on monosynaptic spinal reflex potentials.
Mice were used to record the spinal reflex potentials and to examine the effects of some drugs upon them. In anesthetized mice, laminectomy was performed in the lumbo-sacral region, and monosynaptic reflex potential (MSR) and polysynaptic reflex potential were recorded from the L5 ventral root after stimulation of the L5 dorsal root. Thyrotropin-releasing hormone (TRH) and 1-(4-iodo-2,5-dimethoxyphenyl)-2-aminopropane hydrochloride (DOI) produced transient and long-lasting increases in the MSR amplitude, respectively. Tolperisone hydrochloride and baclofen produced transient and long-lasting MSR depressions, respectively. These results show that mice can be used to record spinal reflex potentials, and that it may be possible to study the spinal cord function of mutant and knockout mice using this method.
Okada
H
H
Laboratory of Pharmacology, Faculty of Pharmaceutical Sciences, Science University of Tokyo, Japan.
Honda
M
M
Ono
H
H
D000662:Amphetamines / Q000494:pharmacology*; D000818:Animals; D001418:Baclofen / Q000494:pharmacology*; D005071:Evoked Potentials / Q000187:drug effects; D008297:Male; D051379:Mice; D009125:Muscle Relaxants, Central / Q000494:pharmacology*; D012023:Reflex, Monosynaptic / Q000187:drug effects*; D017366:Serotonin Receptor Agonists / Q000494:pharmacology*; D013116:Spinal Cord / Q000187:drug effects*; D013973:Thyrotropin-Releasing Hormone / Q000494:pharmacology*; D014049:Tolperisone / Q000494:pharmacology*
D000662:Amphetamines; D009125:Muscle Relaxants, Central; D017366:Serotonin Receptor Agonists; D013973:Thyrotropin-Releasing Hormone; D014049:Tolperisone; D001418:Baclofen; C015952:4-iodo-2,5-dimethoxyphenylisopropylamine