Idiotypic-anti-idiotypic B cell interactions generated against a protective antigen of a morbillivirus in mice.
The idiotypic network theory (N. K. Jerne, Ann. Immunol. 125, 373-389, 1974) predicts that any antibody that can be made by an individual would have its preexisting specific complementary B cells in its germline repertoire. We transplanted syngeneic BALB/c mice with live hybridoma cells and demonstrated the simultaneous presence of interacting idiotypic and anti-idiotypic B cells in an individual animal by immuno-cytoadherence assays. Furthermore, we demonstrate that interacting B cells displaying idiotypic and anti-idiotypic antibodies are subjected to lysis by complement. It is therefore tempting to speculate that this complement-sensitive interaction between idiotypic and complementary anti-idiotypic B cells in vivo may provide a mechanism for the regulation of B cell populations.
Mitra-Kaushik
S
S
Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, 560012, India.
Shaila
M S
MS
Karande
A
A
Nayak
R
R
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D000956:Antigens, Viral / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D002448:Cell Adhesion / Q000276:immunology; D003165:Complement System Proteins / Q000378:metabolism; D005260:Female; D006023:Glycoproteins / Q000276:immunology*; D006389:Hemagglutinins, Viral; D006825:Hybridomas; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D018448:Models, Immunological; D012301:Rinderpest / Q000517:prevention & control; D013154:Spleen / Q000166:cytology / Q000276:immunology; D014764:Viral Proteins / Q000276:immunology*
D000888:Antibodies, Anti-Idiotypic; D000956:Antigens, Viral; D006023:Glycoproteins; D006389:Hemagglutinins, Viral; D007130:Immunoglobulin Idiotypes; D014764:Viral Proteins; C079749:hemagglutinin protein, Rinderpest virus; D003165:Complement System Proteins