title: Utility of 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenyl-amino)carbonyl]-2H-tetrazolium hydroxide (XTT) and minimum effective concentration assays in the determination of antifungal susceptibility of Aspergillus fumigatus to the lipopeptide class compounds.
abstract: The susceptibility of Aspergillus fumigatus to mulundocandin, an echinocandin-like compound, and other antifungal agents was assessed by the National Committee for Clinical Laboratory Standards (NCCLS) M38-P method, a 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenyl-amino)carbonyl]-2H-tetrazolium hydroxide (XTT)-based colorimetric assay, and determination of morphologic alterations by microscopy. In contrast to the NCCLS M38-P method, which does not predict the activity in vivo, the XTT-based assay showed that A. fumigatus is susceptible to mulundocandin. Thus, the XTT-based assay might be useful for determination of the susceptibilities of molds to echinocandins. Further evaluation is warranted.
authors: Aventis, F-93235 Romainville, France.
authors: S P
authors: SP
authors: Hawser
authors: C
authors: C
authors: Jessup
authors: J
authors: J
authors: Vitullo
authors: M A
authors: MA
authors: Ghannoum
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D001232:Aspergillus fumigatus / Q000187:drug effects*; D054714:Echinocandins; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods / Q000592:standards; D010456:Peptides, Cyclic / Q000494:pharmacology*; D013778:Tetrazolium Salts / Q000494:pharmacology*
chemical_list: D000935:Antifungal Agents; D054714:Echinocandins; D010456:Peptides, Cyclic; D013778:Tetrazolium Salts; C059087:2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-((phenylamino)carbonyl)-2H-tetrazolium hydroxide; C052098:mulundocandin