title: Identification and localization of connexin26 within the photoreceptor-horizontal cell synaptic complex.
abstract: Connexin26 (Cx26) is a member of the family of integral membrane proteins that normally form intercellular gap junctional channels. We have used Western blotting, immunofluorescence, immunoelectron microscopy, and single-cell reverse-transcriptase polymerase chain reaction amplification (RT-PCR) to analyze the expression and cellular localization of Cx26 in the carp retina. In the outer plexiform layer, strong clustered Cx26 immunolabeling was concentrated at and restricted to the terminal dendrites of horizontal cells. Single-cell RT-PCR confirmed the expression of Cx26 in carp retinal horizontal cells. 248-bp fragments amplified from cDNAs of four different horizontal cells were cloned and each nucleotide sequence encodes a protein fragment (AA 104-185) with highly significant homology to rat and mouse Cx26. Immunoelectron microscopy revealed that only the invaginating dendrites of horizontal cells in intimate lateral association with the presynaptic ribbon complex were labeled. No labeling was found at the photoreceptor membrane and there was no septalaminar structure, indicative of gap junctions, between photoreceptors and horizontal cells. The focal location of Cx26 at the membrane of the dendritic tips of horizontal cells and the lack of gap junctional morphology suggests that Cx26 might form hemichannels.
authors: Department of Neurobiology, University of Oldenburg, Germany. ulrike.janssen.bienhold@uni-oldenburg.de
authors: U
authors: U
authors: Janssen-Bienhold
authors: K
authors: K
authors: Schultz
authors: A
authors: A
authors: Gellhaus
authors: P
authors: P
authors: Schmidt
authors: J
authors: J
authors: Ammermüller
authors: R
authors: R
authors: Weiler
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D002347:Carps; D000072259:Connexin 26; D017630:Connexins / Q000032:analysis*; D003712:Dendrites / Q000737:chemistry; D019084:Fluorescent Antibody Technique, Indirect; D017629:Gap Junctions / Q000737:chemistry; D008856:Microscopy, Fluorescence; D016253:Microscopy, Immunoelectron; D008969:Molecular Sequence Data; D009474:Neurons / Q000737:chemistry* / Q000166:cytology / Q000648:ultrastructure; D020419:Photoreceptor Cells, Vertebrate / Q000737:chemistry* / Q000166:cytology / Q000648:ultrastructure; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid
chemical_list: D017630:Connexins; C000607044:Gjb2 protein, rat; D000072259:Connexin 26