The distribution of cyclooxygenase-1 in human temporomandibular joint samples: an immunohistochemical study.
Cyclooxygenase-1,2 (COX-1,2) or prostaglandin (PG) H synthase, is the first enzyme of the pathway in which arachidonic acid is oxidized to PGs. Thus, we examined the expression of COX-1 in 16 human temporomandibular joint (TMJ) samples with internal derangement and in 10 control specimens by an immunohistological technique using paraffin-embedded tissue and specific antihuman COX-1 polyclonal antibody. There was obvious distinction of COX-1 immunoreactivity between the control specimens and internal derangement cases, at the endothelial cells and fibroblasts, in the region of posterior and/or anterior loose connective tissues and synovial membrane. The findings of the present study suggest that COX-1 might be an important mechanism for maintaining normal homeostasis at the endothelial cells and fibroblasts with internal derangement of TMJ.
Yoshida
H
H
Department of Oral and Maxillofacial Surgery, Fukui Prefectural Hospital, Fukui, Japan.
Fukumura
Y
Y
Fujita
S
S
Nishida
M
M
Iizuka
T
T
D000328:Adult; D000368:Aged; D000906:Antibodies; D001095:Arachidonic Acids / Q000378:metabolism; D002196:Capillaries / Q000201:enzymology / Q000473:pathology; D003238:Connective Tissue / Q000201:enzymology / Q000473:pathology; D051545:Cyclooxygenase 1; D004730:Endothelium, Vascular / Q000201:enzymology / Q000473:pathology; D005260:Female; D005347:Fibroblasts / Q000201:enzymology / Q000473:pathology; D006706:Homeostasis / Q000502:physiology; D006801:Humans; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000032:analysis*; D004204:Joint Dislocations / Q000201:enzymology / Q000473:pathology; D008297:Male; D008565:Membrane Proteins; D008875:Middle Aged; D010003:Osteoarthritis / Q000201:enzymology / Q000473:pathology; D011451:Prostaglandin-Endoperoxide Synthases / Q000032:analysis*; D013583:Synovial Membrane / Q000201:enzymology / Q000473:pathology; D013704:Temporomandibular Joint / Q000166:cytology / Q000201:enzymology*; D019224:Temporomandibular Joint Disc / Q000201:enzymology / Q000473:pathology; D013705:Temporomandibular Joint Disorders / Q000201:enzymology* / Q000473:pathology
D000906:Antibodies; D001095:Arachidonic Acids; D007527:Isoenzymes; D008565:Membrane Proteins; D051545:Cyclooxygenase 1; C496511:PTGS1 protein, human; D011451:Prostaglandin-Endoperoxide Synthases