Inhibition of androgen receptor-mediated transcription by amino-terminal enhancer of split.
A yeast two-hybrid assay has identified an androgen-dependent interaction of androgen receptor (AR) with amino-terminal enhancer of split (AES), a member of the highly conserved Groucho/TLE family of corepressors. Full-length AR, as well as the N-terminal fragment of AR, showed direct interactions with AES in in vitro protein-protein interaction assays. AES specifically inhibited AR-mediated transcription in a well-defined cell-free transcription system and interacted specifically with the basal transcription factor (TFIIE) in HeLa nuclear extract. These observations implicate AES as a selective repressor of ligand-dependent AR-mediated transcription that acts by directly interacting with AR and by targeting the basal transcription machinery.
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Laboratory of Biochemistry and Molecular Biology, The Rockefeller University, New York, New York 10021, USA.
Li
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Roeder
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Wang
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D059002:Androgen Receptor Antagonists; D056970:Co-Repressor Proteins; D004847:Epithelial Cells / Q000166:cytology; D005786:Gene Expression Regulation; D006367:HeLa Cells; D006801:Humans; D008297:Male; D011467:Prostate / Q000166:cytology; D011506:Proteins; D011944:Receptors, Androgen / Q000235:genetics / Q000378:metabolism*; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology; D019473:Transcription Factors, TFII; D014158:Transcription, Genetic
D059002:Androgen Receptor Antagonists; D056970:Co-Repressor Proteins; D011506:Proteins; D011944:Receptors, Androgen; D012097:Repressor Proteins; C498445:TLE5 protein, human; D014157:Transcription Factors; D019473:Transcription Factors, TFII; C066644:transcription factor TFIIE