A review of factors affecting the performances of latex agglutination tests.
The present review describes the different strategies followed to improve the performance of latex agglutination tests. The analysis is mainly focused on the diverse parameters that affect the final colloidal stability of the immunoprotein-latex system. These parameters include: the surface properties of polymer carriers; the different kind of antibodies usually employed; the use of BSA as stabilizer; the co-adsorption of various macromolecules (BSA, surfactants and lipids) and antibodies; recent approaches to colloidal stability at high ionic strengths due to hydration forces; and the covalent coupling of antibodies on functionalized latex particles. Special emphasis is given to the relation between electrophoretic mobility and the colloidal stability of the sensitized particles and how this knowledge can be utilized for a better understanding of the immunoagglutination kinetic.
Ortega-Vinuesa
J L
JL
Department of Applied Physics, University of Granada, Spain.
Bastos-González
D
D
D000327:Adsorption; D000818:Animals; D000906:Antibodies; D000937:Antigen-Antibody Reactions; D002417:Cattle; D003102:Colloids; D004563:Electrochemistry; D006801:Humans; D007841:Latex Fixation Tests / Q000379:methods*; D008055:Lipids; D009994:Osmolar Concentration; D011108:Polymers; D012710:Serum Albumin, Bovine; D013499:Surface Properties; D013501:Surface-Active Agents
D000906:Antibodies; D003102:Colloids; D008055:Lipids; D011108:Polymers; D013501:Surface-Active Agents; D012710:Serum Albumin, Bovine