Rational approaches to the design of cationic gemini surfactants for gene delivery.
We report a new class of amphiphilic gemini surfactants as vehicles for gene delivery into cells, and the beginnings of a systematic structure-activity study. Preliminary results suggest that combining gemini surfactants with dioleoylphosphatidylethanolamine (DOPE) should allow the preparation of liposomes of various sizes and lipid compositions. Control of such colloidal changes could be as significant as the changes in the molecular composition of the gemini surfactants in delivering optimum gene expression in animal models.
McGregor
C
C
University Chemical Laboratory, Lensfield Road, Cambridge CB2 1EW, U.K.
Perrin
C
C
Monck
M
M
Camilleri
P
P
Kirby
A J
AJ
D000595:Amino Acid Sequence; D000818:Animals; D016466:CHO Cells; D002478:Cells, Cultured; D006224:Cricetinae; D004247:DNA / Q000008:administration & dosage* / Q000378:metabolism; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000737:chemistry*; D006801:Humans; D008081:Liposomes / Q000138:chemical synthesis*; D008156:Luciferases / Q000235:genetics / Q000378:metabolism*; D008239:Lysine / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008563:Membrane Lipids / Q000737:chemistry*; D051379:Mice; D008854:Microscopy, Electron; D015394:Molecular Structure; D009132:Muscles / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D009447:Neuroblastoma / Q000201:enzymology; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry*; D010714:Phosphatidylethanolamines / Q000737:chemistry*; D012694:Serine / Q000737:chemistry; D013329:Structure-Activity Relationship; D013501:Surface-Active Agents / Q000138:chemical synthesis* / Q000737:chemistry*; D014407:Tumor Cells, Cultured
D008081:Liposomes; D008563:Membrane Lipids; D010455:Peptides; D010714:Phosphatidylethanolamines; D013501:Surface-Active Agents; C094877:dioleoyl phosphatidylethanolamine; D012694:Serine; D004247:DNA; D008156:Luciferases; D008239:Lysine