title: In utero ethanol exposure causes mitochondrial dysfunction, which can result in apoptotic cell death in fetal brain: a potential role for 4-hydroxynonenal.
abstract: BACKGROUND
  In utero ethanol exposure causes abnormal fetal brain development that may partly be due to enhanced cell death. The mechanisms underlying this remain to be defined, but ethanol-induced oxidative stress may play a role. The following studies investigated the effects of short-term in utero ethanol exposure on fetal brain mitochondrial events that are known to elicit apoptotic cell death. Evidence is presented suggesting that 4-hydroxynonenal (HNE), a toxic product of lipid oxidation, is a causal factor in the observed mitochondrial damage.
  
  
  METHODS
  Mitochondria were isolated from control and ethanol-exposed fetal brains (days 17 and 18 of gestation). Permeability transition was determined spectrophotometrically, and cytochrome c and apoptosis-inducing factor (AIF) release were assessed by Western blotting. Caspase-3 activity and DNA fragmentation were determined both as markers for mitochondrially mediated apoptosis and as consequences of cytochrome c and AIF release.
  
  
  RESULTS
  Maternal ethanol intake caused an increase in mitochondrial permeability transition, and this was accompanied by cytochrome c and AIF release from fetal brain mitochondria that exceeded control values by 62 and 25%, respectively (p < 0.05). In utero ethanol exposure resulted in a 30% increase in caspase-3 activity and a 25% increase in DNA fragmentation (p < 0.05) in the fetal brain. HNE levels were increased by 23% (p < 0.05) in mitochondria by in vivo ethanol exposure. In vitro treatment of fetal brain mitochondria with HNE (25-100 microM) also caused increases in mitochondrial permeability transition, as well as dose-dependent releases of cytochrome c and AIF.
  
  
  CONCLUSIONS
  These studies illustrate that in utero ethanol exposure can elicit a cascade of events in the fetal brain that are consistent with mitochondrially mediated apoptotic cell death. Additionally, the increase in mitochondrial content of HNE after ethanol intake and the ability of HNE added to fetal brain mitochondria to mimic these effects of in vivo ethanol exposure support a potential role for HNE in the proapoptotic responses to ethanol.
authors: University of Texas Health Science Center at San Antonio, Department of Medicine, Division of Gastroenterology and Nutrition, San Antonio, Texas 78229-3900, USA. ramachandrav@uthscsa.edu
authors: V
authors: V
authors: Ramachandran
authors: A
authors: A
authors: Perez
authors: J
authors: J
authors: Chen
authors: D
authors: D
authors: Senthil
authors: S
authors: S
authors: Schenker
authors: G I
authors: GI
authors: Henderson
mesh_terms: D000447:Aldehydes / Q000032:analysis / Q000378:metabolism*; D000818:Animals; D017209:Apoptosis; D051033:Apoptosis Inducing Factor; D001278:Atractyloside / Q000494:pharmacology; D001921:Brain / Q000196:embryology* / Q000378:metabolism / Q000648:ultrastructure*; D002118:Calcium / Q000494:pharmacology; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism; D003574:Cytochrome c Group / Q000378:metabolism; D053938:DNA Fragmentation; D004791:Enzyme Inhibitors / Q000494:pharmacology; D000431:Ethanol / Q000009:adverse effects*; D005260:Female; D005420:Flavoproteins / Q000378:metabolism; D008431:Maternal-Fetal Exchange; D008565:Membrane Proteins / Q000378:metabolism; D008928:Mitochondria / Q000187:drug effects* / Q000502:physiology; D000226:Mitochondrial ADP, ATP Translocases / Q000037:antagonists & inhibitors; D010539:Permeability; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: C494831:Aifm1 protein, rat; D000447:Aldehydes; D051033:Apoptosis Inducing Factor; D003574:Cytochrome c Group; D004791:Enzyme Inhibitors; D005420:Flavoproteins; D008565:Membrane Proteins; D001278:Atractyloside; D000431:Ethanol; D000226:Mitochondrial ADP, ATP Translocases; C505410:Casp3 protein, rat; D053148:Caspase 3; D020169:Caspases; C027576:4-hydroxy-2-nonenal; D002118:Calcium