title: Phenotypic characterization of five dendritic cell subsets in human tonsils.
abstract: Heterogeneous expression of several antigens on the three currently defined tonsil dendritic cell (DC) subsets encouraged us to re-examine tonsil DCs using a new method that minimized DC differentiation and activation during their preparation. Three-color flow cytometry and dual-color immunohistology was used in conjunction with an extensive panel of antibodies to relevant DC-related antigens to analyze lin(-) HLA-DR(+) tonsil DCs. Here we identify, quantify, and locate five tonsil DC subsets based on their relative expression of the HLA-DR, CD11c, CD13, and CD123 antigens. In situ localization identified four of these DC subsets as distinct interdigitating DC populations. These included three new interdigitating DC subsets defined as HLA-DR(hi) CD11c(+) DCs, HLA-DR(mod) CD11c(+) CD13(+) DCs, and HLA-DR(mod) CD11c(-) CD123(-) DCs, as well as the plasmacytoid DCs (HLA-DR(mod) CD11c(-) CD123(+)). These subsets differed in their expression of DC-associated differentiation/activation antigens and co-stimulator molecules including CD83, CMRF-44, CMRF-56, 2-7, CD86, and 4-1BB ligand. The fifth HLA-DR(mod) CD11c(+) DC subset was identified as germinal center DCs, but contrary to previous reports they are redefined as lacking the CD13 antigen. The definition and extensive phenotypic analysis of these five DC subsets in human tonsil extends our understanding of the complexity of DC biology.
authors: Hematology/Immunology Research Group, Christchurch Hospital, Christchurch, New Zealand.
authors: K L
authors: KL
authors: Summers
authors: B D
authors: BD
authors: Hock
authors: J L
authors: JL
authors: McKenzie
authors: D N
authors: DN
authors: Hart
mesh_terms: D018826:CD13 Antigens / Q000378:metabolism; D003713:Dendritic Cells / Q000145:classification* / Q000502:physiology*; D005434:Flow Cytometry; D006684:HLA-DR Antigens / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D016167:Integrin alphaXbeta2 / Q000378:metabolism; D053650:Interleukin-3 Receptor alpha Subunit; D014066:Palatine Tonsil / Q000166:cytology* / Q000378:metabolism; D010641:Phenotype; D016185:Receptors, Interleukin-3 / Q000378:metabolism
chemical_list: D006684:HLA-DR Antigens; C115022:IL3RA protein, human; D016167:Integrin alphaXbeta2; D053650:Interleukin-3 Receptor alpha Subunit; D016185:Receptors, Interleukin-3; D018826:CD13 Antigens