title: Production of beta-thujaplicin in Cupressus lusitanica suspension cultures fed with organic acids and monoterpenes.
abstract: Effects of some organic acids and monoterpenes on production of beta-thujaplicin were studied in Cupressus lusitanica suspension cultures. The fungal elicitor-induced biosynthesis of beta-thujaplicin was promoted by the feedings of malate, pyruvate, fumarate, succinate, and acetate. These results suggest some relationships between acetate/pyruvate metabolism and beta-thujaplicin biosynthesis, or between tricarboxylic acid cycle and beta-thujaplicin biosynthesis. Feedings of C. lusitanica suspension cultures with some monoterpenes inhibited elicitor-triggered beta-thujaplicin biosynthesis, but 2-carene and terpinyl acetate feedings significantly improved the beta-thujaplicin production of C. lusitanica suspension cultures. These results indicate a possible involvement of terpinyl acetate and 2-carene in beta-thujaplicin biosynthesis, as well as potential uses of these monoterpenes in large-scale beta-thujaplicin production.
authors: Department of Forest Products Science, Faculty of Agriculture, Kyushu University, Fukuoka, Japan.
authors: J
authors: J
authors: Zhao
authors: K
authors: K
authors: Fujita
authors: K
authors: K
authors: Sakai
mesh_terms: D000143:Acids / Q000378:metabolism*; D018929:Cell Culture Techniques; D002952:Citric Acid Cycle; D003470:Culture Media; D007700:Kinetics; D039821:Monoterpenes; D013729:Terpenes / Q000378:metabolism*; D014197:Trees / Q000166:cytology / Q000378:metabolism*; D014334:Tropolone / Q000031:analogs & derivatives / Q000378:metabolism*
chemical_list: D000143:Acids; D003470:Culture Media; D039821:Monoterpenes; D013729:Terpenes; D014334:Tropolone; C009479:beta-thujaplicin