Screening for microorganisms that produce only endo-inulinase.
Sixteen fungal strains reported in the literature as endo-inulinase producers and three bacterial strains, isolated from the dahlia rhizosphere, were analysed for endo-inulinase production. From four isolated strains (one fungus and three bacteria) the results were evaluated in terms of substrate consumption, cell growth and production of endo-inulinases. All three bacterial strains were sole endo-inulinase producers and, among these, strain Paenibacillus sp. CDB 003 was the most suitable for endo-inulinase production, as this enzyme produced inulobiose as the principal substrate as well as inulo-oligosaccharides with polymerisation degrees of 3-5.
Gern
R M
RM
Universidade da Região de Joinville, SC, Brazil.
Furlan
S A
SA
Ninow
J L
JL
Jonas
R
R
D001419:Bacteria / Q000201:enzymology* / Q000254:growth & development / Q000302:isolation & purification; D001709:Biotechnology; D005658:Fungi / Q000201:enzymology* / Q000254:growth & development; D006026:Glycoside Hydrolases / Q000096:biosynthesis*; D016937:Gram-Positive Endospore-Forming Rods / Q000201:enzymology / Q000254:growth & development / Q000302:isolation & purification; D012988:Soil Microbiology; D013045:Species Specificity
D006026:Glycoside Hydrolases; C023086:inulinase