The TFIID components human TAF(II)140 and Drosophila BIP2 (TAF(II)155) are novel metazoan homologues of yeast TAF(II)47 containing a histone fold and a PHD finger.
The RNA polymerase II transcription factor TFIID comprises the TATA binding protein (TBP) and a set of TBP-associated factors (TAF(II)s). TFIID has been extensively characterized for yeast, Drosophila, and humans, demonstrating a high degree of conservation of both the amino acid sequences of the constituent TAF(II)s and overall molecular organization. In recent years, it has been assumed that all the metazoan TAF(II)s have been identified, yet no metazoan homologues of yeast TAF(II)47 (yTAF(II)47) and yTAF(II)65 are known. Both of these yTAF(II)s contain a histone fold domain (HFD) which selectively heterodimerizes with that of yTAF(II)25. We have cloned a novel mouse protein, TAF(II)140, containing an HFD and a plant homeodomain (PHD) finger, which we demonstrated by immunoprecipitation to be a mammalian TFIID component. TAF(II)140 shows extensive sequence similarity to Drosophila BIP2 (dBIP2) (dTAF(II)155), which we also show to be a component of Drosophila TFIID. These proteins are metazoan homologues of yTAF(II)47 as their HFDs selectively heterodimerize with dTAF(II)24 and human TAF(II)30, metazoan homologues of yTAF(II)25. We further show that yTAF(II)65 shares two domains with the Drosophila Prodos protein, a recently described potential dTAF(II). These conserved domains are critical for yTAF(II)65 function in vivo. Our results therefore identify metazoan homologues of yTAF(II)47 and yTAF(II)65.
Gangloff
Y G
YG
Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS/INSERM/ULP, 67404 Illkirch Cédex, C.U. de Strasbourg, France.
Pointud
J C
JC
Thuault
S
S
Carré
L
L
Romier
C
C
Muratoglu
S
S
Brand
M
M
Tora
L
L
Couderc
J L
JL
Davidson
I
I
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D019556:COS Cells; D002176:Candida albicans / Q000737:chemistry; D003001:Cloning, Molecular; D017124:Conserved Sequence; D019281:Dimerization; D004330:Drosophila; D029721:Drosophila Proteins; D019143:Evolution, Molecular; D005816:Genetic Complementation Test; D006367:HeLa Cells; D006657:Histones / Q000737:chemistry*; D006801:Humans; D017403:In Situ Hybridization; D051379:Mice; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D010957:Plasmids / Q000378:metabolism; D011233:Precipitin Tests; D017434:Protein Structure, Tertiary; D029701:Saccharomyces cerevisiae Proteins; D012469:Salivary Glands / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D035381:TATA-Binding Protein Associated Factors; D013696:Temperature; D013997:Time Factors; D015534:Trans-Activators / Q000737:chemistry; D035362:Transcription Factor TFIID; D014157:Transcription Factors / Q000737:chemistry*; D019473:Transcription Factors, TFII / Q000737:chemistry*; D020798:Two-Hybrid System Techniques; D014981:Xenopus; D015027:Zebrafish
C432688:BIP2 protein, Drosophila; D029721:Drosophila Proteins; D006657:Histones; D029701:Saccharomyces cerevisiae Proteins; C432690:TAF3 protein, S cerevisiae; C432689:TAF3 protein, human; D035381:TATA-Binding Protein Associated Factors; C418995:Taf8 protein, Drosophila; D015534:Trans-Activators; D035362:Transcription Factor TFIID; D014157:Transcription Factors; D019473:Transcription Factors, TFII