Rapid approach to determine rrn arrangement in Salmonella serovars.
A PCR method was developed by which to rapidly and accurately determine the rrn arrangement of Salmonella enterica serovars. Primers were designed to the genomic regions flanking each of the seven rrn operons. PCR analysis using combinations of these primers will distinguish each of the possible arrangements of the rrn skeleton.
Helm
R A
RA
Department of Microbiology, University of Illinois, Urbana, Illinois 61801, USA.
Maloy
S
S
D002874:Chromosome Mapping / Q000379:methods*; D017931:DNA Primers; D005798:Genes, Bacterial; D020459:Genes, rRNA; D016133:Polymerase Chain Reaction / Q000379:methods*; D012335:RNA, Ribosomal / Q000235:genetics*; D019779:Salmonella enterica / Q000145:classification / Q000235:genetics*; D013997:Time Factors
D017931:DNA Primers; D012335:RNA, Ribosomal