[Establishment of ELISA with TT virus(TTV) and its application to patients with hepatitis and healthy people].
OBJECTIVE
To establish the EIA method for detection of antibody to TT virus (anti-TTV) and to investigate the distribution of anti-TTV in patients with hepatitis and in healthy people.


METHODS
The ELISA method using the recombinant expressed product of truncated gene of TTV ORF1 as antigen was developed and was applied to detect anti-TTV in patients with hepatitis and in healthy control. The TTV DNA were detected by nested-PCR.


RESULTS
The positive rates of anti-TTV antibodies in different populations were as follows: 1.3% in healthy people, 10.5% in patients with hepatitis A, 12.5% in patients with hepatitis B, 8.3% in patients with hepatitis C, 7.7% in patients with hepatitis D, 12.0% in patients with hepatitis E, 6.5% in patients with hepatitis G, 32.3% in patients with hepatitis non-A to G. The positive rate of TTV DNA was significantly related to that of anti-TTV (P < 0.05).


CONCLUSIONS
The anti-TTV antibodies were detected positive in patients with hepatitis and healthy people, however, its positive rate in patients with hepatitis non-A to G was significantly higher than that in other groups. The fact that anti-TTV antibodies coexist with TTV DNA suggests that the antibody to TTV ORF1 antigen may be an infectious marker of TTV similar to that of anti-HCV.
Chen
X
X
Shenzhen Eastlake Hospital, Guangdong 518020, China.
Li
M
M
Wang
H
H
D000914:Antibodies, Viral; D004797:Enzyme-Linked Immunosorbent Assay; D006525:Hepatitis, Viral, Human / Q000175:diagnosis / Q000821:virology; D006801:Humans; D022783:Torque teno virus / Q000235:genetics / Q000276:immunology*
D000914:Antibodies, Viral