title: Functional analysis of secreted and transmembrane proteins critical to mouse development.
abstract: We describe the successful application of a modified gene-trap approach, the secretory trap, to systematically analyze the functions in vivo of large numbers of genes encoding secreted and membrane proteins. Secretory-trap insertions in embryonic stem cells can be transmitted to the germ line of mice with high efficiency and effectively mutate the target gene. Of 60 insertions analyzed in mice, one-third cause recessive lethal phenotypes affecting various stages of embryonic and postnatal development. Thus, secretory-trap mutagenesis can be used for a genome-wide functional analysis of cell signaling pathways that are critical for normal mammalian development and physiology.
authors: Department of Molecular and Cell Biology, University of California, Berkeley, California 94720, USA.
authors: K J
authors: KJ
authors: Mitchell
authors: K I
authors: KI
authors: Pinson
authors: O G
authors: OG
authors: Kelly
authors: J
authors: J
authors: Brennan
authors: J
authors: J
authors: Zupicich
authors: P
authors: P
authors: Scherz
authors: P A
authors: PA
authors: Leighton
authors: L V
authors: LV
authors: Goodrich
authors: X
authors: X
authors: Lu
authors: B J
authors: BJ
authors: Avery
authors: P
authors: P
authors: Tate
authors: K
authors: K
authors: Dill
authors: E
authors: E
authors: Pangilinan
authors: P
authors: P
authors: Wakenight
authors: M
authors: M
authors: Tessier-Lavigne
authors: W C
authors: WC
authors: Skarnes
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000166:cytology; D001947:Breeding; D005804:Genes, Lethal; D005822:Genetic Vectors; D005838:Genotype; D008565:Membrane Proteins / Q000235:genetics*; D051379:Mice / Q000235:genetics*; D008967:Molecular Biology / Q000379:methods*; D016254:Mutagenesis, Insertional; D010641:Phenotype; D016133:Polymerase Chain Reaction; D011506:Proteins / Q000378:metabolism*; D012641:Selection, Genetic; D016324:Sequence Tagged Sites; D013234:Stem Cells / Q000166:cytology
chemical_list: D008565:Membrane Proteins; D011506:Proteins