title: Differential effects of neuropeptide Y (NPY) on leukocyte subsets in the blood: mobilization of B-1-like B-lymphocytes and activated monocytes.
abstract: Sympathetic nervous system activation mobilizes leukocytes but it is unknown whether the concomitant neuropeptide Y (NPY)-release also alters blood leukocyte counts. Using chronic intravenous (i.v.) cannulation of freely moving rats and flow cytometry, time-, dose- and subset-specific effects of NPY on blood leukocytes were investigated 1-15 min after injection: High-dose NPY increases leukocytes numbers by preferentially mobilizing CD4(+) T-cells, activated NKR-P1A(+) monocytes and NK-cells. Low-dose NPY significantly decreases B-lymphocyte and NK-cell numbers. Furthermore, NPY dose-dependently mobilizes a previously undetected IgM(low)CD5(+)CD11b(+) B-cell subpopulation in rats ("B1-like" B-lymphocytes). These data suggest a role for the sympathetic neurotransmitter NPY in neuroimmune alterations in vivo.
authors: Department of Functional and Applied Anatomy, OE 4120, Hannover Medical School, Carl-Neuberg-Strasse 1, 30625, Hannover, Germany.
authors: S
authors: S
authors: Bedoui
authors: S
authors: S
authors: Kuhlmann
authors: H
authors: H
authors: Nave
authors: J
authors: J
authors: Drube
authors: R
authors: R
authors: Pabst
authors: S
authors: S
authors: von Hörsten
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects* / Q000502:physiology; D002465:Cell Movement / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D007958:Leukocyte Count; D008297:Male; D009000:Monocytes / Q000187:drug effects* / Q000502:physiology; D009478:Neuropeptide Y / Q000494:pharmacology*; D051381:Rats; D011917:Rats, Inbred Lew
chemical_list: D009478:Neuropeptide Y