[The demonstration of natural hybridization between two swallowtail species Parnassius nomion and Parnassius bremeri (Lepidoptera, Papilionidae) using RAPD-PCR technique].
Genetic evidence for interspecific hybridization between Parnassius nomion and Parnassius bremeri in nature is presented. To demonstrate hybridization between these species, RAPD analysis was used. By testing 25 decamer primers, three and two diagnostic markers were revealed for P. nomion and P. bremeri, respectively. Out of 28 animals examined, 4 were shown to be interspecific hybrids. According to the distribution of diagnostic markers, the interspecific hybrids were intermediate with regard to the parental species. Ecological and biological characteristics of two swallowtail species that promote their hybridization in nature are discussed.
Zakharov
E V
EV
Institute of Biology and Soil Sciences, Russian Academy of Sciences, Vladivostok, 690022 Russia. entomol@online.marine.su
D000818:Animals; D017931:DNA Primers; D017344:Genes, Insect; D005819:Genetic Markers; D016678:Genome; D006824:Hybridization, Genetic; D007915:Lepidoptera / Q000145:classification / Q000235:genetics*
D017931:DNA Primers; D005819:Genetic Markers