title: A coiled-coil from the RNA polymerase beta' subunit allosterically induces selective nontemplate strand binding by sigma(70).
abstract: For transcription to initiate, RNA polymerase must recognize and melt promoters. Selective binding to the nontemplate strand of the -10 region of the promoter is central to this process. We show that a 48 amino acid (aa) coiled-coil from the beta' subunit (aa 262--309) induces sigma(70) to perform this function almost as efficiently as core RNA polymerase itself. We provide evidence that interaction between the beta' coiled-coil and region 2.2 of sigma(70) promotes an allosteric transition that allows sigma(70) to selectively recognize the nontemplate strand. As the beta' 262--309 peptide can function with the previously crystallized portion of sigma(70), nontemplate recognition can be reconstituted with only 47 kDa, or 1/10 of holoenzyme.
authors: Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94143, USA.
authors: B A
authors: BA
authors: Young
authors: L C
authors: LC
authors: Anthony
authors: T M
authors: TM
authors: Gruber
authors: T M
authors: TM
authors: Arthur
authors: E
authors: E
authors: Heyduk
authors: C Z
authors: CZ
authors: Lu
authors: M M
authors: MM
authors: Sharp
authors: T
authors: T
authors: Heyduk
authors: R R
authors: RR
authors: Burgess
authors: C A
authors: CA
authors: Gross
mesh_terms: D000494:Allosteric Regulation; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008958:Models, Molecular; D009154:Mutation; D009691:Nucleic Acid Denaturation; D010446:Peptide Fragments / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic; D017434:Protein Structure, Tertiary; D012808:Sigma Factor / Q000737:chemistry / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D004268:DNA-Binding Proteins; D010446:Peptide Fragments; D012808:Sigma Factor; C081775:RNA polymerase sigma 70; D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit