title: Decrease in Cryptosporidium parvum oocyst infectivity in vitro by using the membrane filter dissolution method for recovering oocysts from water samples.
abstract: Exposure of Cryptosporidium parvum oocysts to solutions used for cellulose acetate membrane (CAM) dissolution filtration reduced their infectivity in HCT-8 cells. Ethanol (95% [vol/vol] and 70% [vol/vol]) alone and short exposure times to acetone decreased infectivity. These findings contrast with similar experiments using excystation assays and infectivity in mice.
authors: Department of Environmental Biology, University of Guelph, Guelph, Ontario, Canada N1G 2W1.
authors: R A
authors: RA
authors: Carreno
authors: N J
authors: NJ
authors: Pokorny
authors: S C
authors: SC
authors: Weir
authors: H
authors: H
authors: Lee
authors: J T
authors: JT
authors: Trevors
mesh_terms: D000818:Animals; D002460:Cell Line; D002482:Cellulose / Q000031:analogs & derivatives; D003457:Cryptosporidiosis / Q000469:parasitology; D016785:Cryptosporidium parvum / Q000254:growth & development* / Q000302:isolation & purification / Q000472:pathogenicity*; D005374:Filtration / Q000379:methods; D006801:Humans; D008567:Membranes, Artificial; D014867:Water / Q000469:parasitology*
chemical_list: D008567:Membranes, Artificial; D014867:Water; C005062:acetylcellulose; D002482:Cellulose