title: Estimation of the number of polyhydroxyalkanoate (PHA)-degraders in soil and isolation of degraders based on the method of most probable number (MPN) using PHA-film.
abstract: A new method to estimate the number of polyhydroxyalkanoates (PHA)-degraders in soil and to isolate degraders, called the film-MPN method, is proposed. The incubation time was measured by the first order reaction (FOR) model. This method was used to estimate numbers of poly(3-hydroxybutyrate-co-3-hydroxyvalerate)[P(3HB-co-3HV)]- and poly(3-hydroxyvalerate-co-4-hydroxybutyrate)[P(3HB-co-4HB)]-degraders in garden soil (4.30 x 10(5) and 2.15 x 10(5) aerobic degraders per gram of dry soil, respectively). The number of P(3HB-co-3HV)-degraders in paddy field soil was 5.06 x 10(5) aerobic degraders per gram dry soil. Also, several P(3HB-co-3HV)-degraders were isolated directly from positive-growth tubes of high dilution.
authors: Department of System Engineering of Materials and Life Science, Faculty of Engineering, Toyama University, Japan.
authors: C
authors: C
authors: Song
authors: U
authors: U
authors: Uchida
authors: S
authors: S
authors: Ono
authors: C
authors: C
authors: Shimasaki
authors: M
authors: M
authors: Inoue
mesh_terms: D001673:Biodegradation, Environmental; D018441:Biofilms; D015169:Colony Count, Microbial; D011083:Polycyclic Compounds / Q000378:metabolism*; D012988:Soil Microbiology
chemical_list: D011083:Polycyclic Compounds