Exhaled nitric oxide in patients with PiZZ phenotype-related alpha1-anti-trypsin deficiency.
There is no report of exhaled NO (eNO) in subjects with different phenotypes of alpha1-anti-trypsin (AAT) deficiency. Exhaled nitric oxide was evaluated by means of single-breath chemiluminescence analysis (fractional exhaled concentration at the plateau level [plFE(NO)]) in 40 patients with AAT deficiency. Patients were divided according to the protease inhibitor (Pi) phenotype: PiMZ/MS, n = 25; PiSZ n = 6; PiZZ, n = 9. Nineteen healthy subjects served as controls. Levels of eNO in PiZZ patients were also compared with those of subjects, without AAT deficiency (PiMM), matched for diagnosis, sex, age, smoking habit and forced expiratory volume in 1 sec (FEV1). In AAT deficiency subjects airway hyper-responsiveness to methacholine (PD20 FEV1) was also assessed. plFE(NO) was significantly lower in the PiZZ group (4.5+/-1.4 ppb) than in matched PiMM subjects (8.2+/-3.8 ppb), in healthy controls (9.3+/-2.8 ppb) and in patients of other phenotypes. Dynamic lung volumes and DL(CO) were significantly lower in PiZZ than in other AAT-deficient patients. Bronchial hyper-responsiveness was not different among AAT phenotypes. These results suggest that eNO may be significantly reduced in PiZZ as compared to healthy control subjects and to AAT subjects with other phenotypes, independent of the level of airway obstruction. Whether, at least potentially, eNO may be considered as an early marker of lung involvement in AAT deficiency must be confirmed with studies on larger number of subjects.
Malerba
M
M
Institute of Internal Medicine University of Brescia, Italy.
Clini
E
E
Cremona
G
G
Radaeli
A
A
Bianchi
L
L
Corda
L
L
Pini
L
L
Ricciardolo
F
F
Grassi
V
V
Ambrosino
N
N
Ricclardolo
F
F
D000328:Adult; D000704:Analysis of Variance; D001944:Breath Tests; D001985:Bronchial Provocation Tests; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D005260:Female; D005541:Forced Expiratory Volume; D006801:Humans; D008163:Luminescent Measurements; D008297:Male; D016210:Methacholine Chloride; D009569:Nitric Oxide / Q000378:metabolism*; D010641:Phenotype; D012130:Respiratory Hypersensitivity / Q000175:diagnosis / Q000209:etiology; D018709:Statistics, Nonparametric; D019896:alpha 1-Antitrypsin Deficiency / Q000150:complications / Q000235:genetics / Q000378:metabolism*
D016210:Methacholine Chloride; D009569:Nitric Oxide