title: PKCgamma contributes to a subset of the NMDA-dependent spinal circuits that underlie injury-induced persistent pain.
abstract: In previous studies we provided evidence that the gamma isoform of protein kinase C (PKCgamma) is an important contributor to the increased pain sensitivity that occurs after injury. Here we combined electrophysiological and behavioral approaches in wild-type and PKCgamma-null mice to compare the hyperexcitability of wide dynamic range neurons in lamina V of the spinal cord dorsal horn with the behavioral hyperexcitability produced by the same injury [application of a C-fiber irritant, mustard oil (MO), to the hindpaw]. Wild-type and null mice did not differ in their response to mechanical or thermal stimuli before tissue injury, and the magnitude of the response to the MO stimuli was comparable. In wild-type mice, MO produced a dramatic and progressive enhancement of the response of lamina V neurons to innocuous mechanical and thermal stimuli. The time course of the neuronal hyperexcitability paralleled the time course of the MO-induced behavioral allodynia (nocifensive behavior in response to a previously innocuous mechanical stimulus). Neuronal hyperexcitability was also manifest in the PKCgamma-null mice, but it lasted <30 min. By contrast, the behavioral allodynia produced by MO in the PKCgamma-null mice, although reduced to approximately half that of the wild-type mice, persisted long after the lamina V hyperexcitability had subsided. Because the MO-induced behavioral allodynia was completely blocked by an NMDA receptor antagonist, we conclude that PKCgamma mediates the transition from short- to long-term hyperexcitability of lamina V nociresponsive neurons but that the persistence of injury-induced pain must involve activity within multiple NMDA-dependent spinal cord circuits.
authors: Departments of Anatomy, W. M. Keck Foundation Center for Integrative Neuroscience, University of California, San Francisco, San Francisco, California 94143-0452, USA.
authors: W J
authors: WJ
authors: Martin
authors: A B
authors: AB
authors: Malmberg
authors: A I
authors: AI
authors: Basbaum
mesh_terms: D000200:Action Potentials / Q000187:drug effects / Q000235:genetics; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D004195:Disease Models, Animal; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D006930:Hyperalgesia / Q000139:chemically induced / Q000503:physiopathology / Q000517:prevention & control; D007527:Isoenzymes / Q000172:deficiency* / Q000235:genetics; D051379:Mice; D018345:Mice, Knockout; D009149:Mustard Plant; D016202:N-Methylaspartate / Q000378:metabolism*; D009437:Neuralgia / Q000503:physiopathology*; D010147:Pain Measurement / Q000187:drug effects; D010812:Physical Stimulation; D010936:Plant Extracts / Q000494:pharmacology; D010938:Plant Oils; D020671:Posterior Horn Cells / Q000503:physiopathology; D011493:Protein Kinase C / Q000172:deficiency* / Q000235:genetics; D016194:Receptors, N-Methyl-D-Aspartate / Q000037:antagonists & inhibitors; D013116:Spinal Cord / Q000503:physiopathology*
chemical_list: D018691:Excitatory Amino Acid Antagonists; D007527:Isoenzymes; D010936:Plant Extracts; D010938:Plant Oils; D016194:Receptors, N-Methyl-D-Aspartate; D016202:N-Methylaspartate; C087857:protein kinase C gamma; D011493:Protein Kinase C; C027793:mustard oil