Metabolism of the veterinary fluoroquinolone sarafloxacin by the fungus Mucor ramannianus.
To investigate the microbial biotransformation of veterinary fluoroquinolones, Mucor ramannianus was grown in sucrose/peptone broth with sarafloxacin for 18 days. Cultures were extracted with ethyl acetate and extracts were analyzed by liquid chromatography. The two metabolites (26% and 15% of the A280, respectively) were identified by mass and 1H nuclear magnetic resonance spectra as N-acetylsarafloxacin and desethylene-N-acetylsarafloxacin. The biological formation of desethylene-N-acetylsarafloxacin has not been previously observed.
Parshikov
I A
IA
National Center for Toxicological Research, Food and Drug Administration, Jefferson, AR 72079, USA.
Freeman
J P
JP
Lay
J O
JO
Moody
J D
JD
Williams
A J
AJ
Beger
R D
RD
Sutherland
J B
JB
D000818:Animals; D000890:Anti-Infective Agents / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002939:Ciprofloxacin / Q000031:analogs & derivatives* / Q000378:metabolism*; D024841:Fluoroquinolones; D009089:Mucor / Q000378:metabolism*; D012989:Soil Pollutants / Q000378:metabolism; D014730:Veterinary Medicine / Q000379:methods
D000890:Anti-Infective Agents; D024841:Fluoroquinolones; D012989:Soil Pollutants; D002939:Ciprofloxacin; C047224:sarafloxacin