title: Identification of delta- and mu-type opioid receptors on human and murine dendritic cells.
abstract: The purpose of this study was to evaluate mu- and delta-opioid receptors (OR) on human and murine dendritic cells (DC). Expression of mu- and delta-OR mRNA on DC was demonstrated by RT-PCR. The immunocytochemical and Western blot analyses revealed the expression of OR protein in DC. Radioreceptor assay demonstrated the specific saturated temperature-dependent binding of [3H]-labeled opioid ligand on DC and B(max)=2.8+/-0.3 fmol/10(6) cells and K(D)=4.8+/-1.0 nM were calculated by a Scatchard analysis. Finally, OR ligands DADLE and DAGO dose-dependently modulated the capacity of DC to induce T cell proliferation in an MLR assay. Importantly, expression of functional OR on DC was significantly increased upon TNF-alpha-induced DC maturation. Thus, these data suggest a new mechanism of opioid-dependent neuroendocrine immunomodulation.
authors: National Research Center for Mental Health, 113152, Moscow, Russia.
authors: V P
authors: VP
authors: Makarenkova
authors: C
authors: C
authors: Esche
authors: N V
authors: NV
authors: Kost
authors: G V
authors: GV
authors: Shurin
authors: B S
authors: BS
authors: Rabin
authors: A A
authors: AA
authors: Zozulya
authors: M R
authors: MR
authors: Shurin
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000737:chemistry*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D012333:RNA, Messenger / Q000032:analysis; D017465:Receptors, Opioid, delta / Q000032:analysis* / Q000235:genetics / Q000502:physiology; D017450:Receptors, Opioid, mu / Q000032:analysis* / Q000235:genetics / Q000502:physiology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
chemical_list: D012333:RNA, Messenger; D017465:Receptors, Opioid, delta; D017450:Receptors, Opioid, mu; D014409:Tumor Necrosis Factor-alpha