Dynamic micro-magnetic resonance imaging of liver micrometastasis in mice with a novel liver macromolecular magnetic resonance contrast agent DAB-Am64-(1B4M-Gd)(64).
DAB-Am64-(1B4M-Gd)(64) is a newly synthesized macromolecular liver magnetic resonance imaging (MRI) contrast agent with a polypropylenimine diaminobutane (DAB) dendrimer conjugated with a bifunctional diethylenetriaminepentaacetic acid (DTPA) derivative for complexing Gd(III) atoms. The characteristics of DAB-Am64-(1B4M-Gd)(64), which quickly accumulated in the liver, have been reported recently. In the present study, the dynamic micro-MRI with DAB-Am64-(1B4M-Gd)(64) was obtained in the mouse liver metastasis model using colon carcinoma cells to evaluate the ability to visualize the micrometastatic tumors compared with that using Gd-DTPA. The dynamic micro-MRI with DAB-Am64-(1B4M-Gd)(64) was able to homogeneously enhance the normal liver parenchyma and visualize micrometastatic tumors of 0.3-mm diameter in the liver of the mice with better contrast than that with Gd-DTPA. In conclusion, DAB-Am64-(1B4M-Gd)(64) is a new liver MRI contrast agent potentially useful for diagnosis of micrometastasis in the liver.
Kobayashi
H
H
Department of Diagnostic and Interventional Imagiology, Kyoto University, Kyoto 606-8507, Japan. hisataka@kuhp.kyoto-u.ac.jp
Saga
T
T
Kawamoto
S
S
Sato
N
N
Hiraga
A
A
Ishimori
T
T
Konishi
J
J
Togashi
K
K
Brechbiel
M W
MW
D000818:Animals; D015179:Colorectal Neoplasms / Q000473:pathology; D003287:Contrast Media; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D005260:Female; D019786:Gadolinium DTPA / Q000031:analogs & derivatives* / Q000627:therapeutic use* / Q000633:toxicity; D006801:Humans; D008114:Liver Neoplasms, Experimental / Q000175:diagnosis / Q000473:pathology / Q000556:secondary*; D008279:Magnetic Resonance Imaging; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D014183:Transplantation, Heterologous
D003287:Contrast Media; C430743:DAB-Am64-(1B4M-Gd)64; D019786:Gadolinium DTPA