Real-time PCR as a new tool for quantifying Leishmania infantum in liver in infected mice.
The parasitic loads of mouse livers experimentally infected with Leishmania infantum were determined using a double real-time quantitative PCR test targeted to the parasite DNA polymerase gene and to the mouse brain-derived neutrophic factor gene. The Leishmania DNA copy number was normalized to the number of mouse gene copies in order to quantify the former independently of liver weight. The correlation coefficient with the microtitration method was 0.66. This PCR assay can be considered for experimental pharmaceutical studies.
Bretagne
S
S
Laboratoire de Parasitologie-Mycologie, Hôpital Henri Mondor-APHP and Université Paris XII, Créteil, Paris, France. bretagne@univ-paris12.fr
Durand
R
R
Olivi
M
M
Garin
J F
JF
Sulahian
A
A
Rivollet
D
D
Vidaud
M
M
Deniau
M
M
D000818:Animals; D016054:DNA, Protozoan / Q000032:analysis; D004195:Disease Models, Animal; D005260:Female; D018314:Leishmania infantum / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification; D007898:Leishmaniasis, Visceral / Q000469:parasitology*; D008099:Liver / Q000469:parasitology*; D051379:Mice; D008807:Mice, Inbred BALB C; D016133:Polymerase Chain Reaction / Q000379:methods*; D013997:Time Factors
D016054:DNA, Protozoan