A unique murine monoclonal antibody recognizing HLA-B53, B37, B51, B52, +/-B44.
Monoclonal antibodies have played an important role in studying the biochemistry of the HLA-Class I molecules. Some murine anti-HLA mAbs can identify configurations of HLA epitopes that have never been reported in human allosera. One of these configurations is identified by an IgM mAb designated as: BHA-1441. This antibody was produced using a lymphoblastoid cell line typed as: A*02, A*25; B*38, B*4402/4405; C*0501, C*07, BW4, as the immunogen. A lymphocytotoxicity test of this mAb over a panel of 109 frozen, 452 fresh and, later, 44 DNA typed T cells revealed its specificity as B53, 37, 51, 52, +/- 44. All of the antigens recognized by this mAb share the Bw4 motif at positions 81-83, except for the HLA-B37, which shares only 82L and 83R. Furthermore, while B37 and B44 cross-react due to the aspartic acid (D) substitution at position 156, the reactivity with B53, B5 (51,52), B37 and 60% of B44 cells, makes it unlikely that the target epitope could be due only to the primary amino-acid sequence. The antibody-binding site might involve changes in tertiary structure and peptides bound by the MHC. BHA-1441 is an interesting tool to study and type the HLA-B53 antigen and its cross-reactive epitopes.
Etessami
S
S
One Lambda, Inc, Canoga Park, California 91303, USA. setessami@llambda.com
Tarsitani
C
C
Lias
M
M
Broadous
M
M
Conger
N
N
Randol
G
G
Delory
M
M
Bahrami
S
S
Lee
J H
JH
Randall
G
G
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism*; D000937:Antigen-Antibody Reactions; D002461:Cell Line, Transformed; D003601:Cytotoxicity Tests, Immunologic; D005260:Female; D006680:HLA Antigens / Q000276:immunology*; D015235:HLA-B Antigens / Q000276:immunology*; D059912:HLA-B37 Antigen; D059948:HLA-B44 Antigen; D059949:HLA-B51 Antigen; D059950:HLA-B52 Antigen; D006650:Histocompatibility Testing; D006801:Humans; D006825:Hybridomas; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data
D000911:Antibodies, Monoclonal; D006680:HLA Antigens; D015235:HLA-B Antigens; D059912:HLA-B37 Antigen; D059948:HLA-B44 Antigen; D059949:HLA-B51 Antigen; D059950:HLA-B52 Antigen; C078438:HLA-B53 antigen