Rsk2 allosterically activates estrogen receptor alpha by docking to the hormone-binding domain.
We describe a novel mechanism for transcriptional regulation, in which docking of p90 ribosomal S6 kinase 2 (Rsk2) to the hormone-binding domain (HBD) of estrogen receptor alpha (ERalpha) induces a conformational change that enhances the transcriptional activation function contained in the HBD. A constitutively active mutant of Rsk2 specifically enhances ERalpha-mediated transcription by phosphorylation of Ser167 in ERalpha and by physically associating with residues 326-394 of the ERalpha HBD. The anti-estrogen 4-hydroxytamoxifen blocks Rsk2-mediated activation of ERalpha, by inducing a conformation of ERalpha in which the Rsk2 docking site is masked. Transcriptional activation and docking are specific for ERalpha and do not occur with the related isoform, ERbeta. ERalpha phosphorylation, docking and transcriptional activation are regulated by the Rsk2 N-terminal kinase domain. The allosteric regulation of a target protein, independent of phosphorylation, may be paradigmatic of a general function for protein kinase docking sites.
Clark
D E
DE
Center for Cell Signaling and PharmaBiologicals, University of Virginia, Charlottesville, VA 22908-0577, USA.
Poteet-Smith
C E
CE
Smith
J A
JA
Lannigan
D A
DA
D000494:Allosteric Regulation; D019943:Amino Acid Substitution; D000818:Animals; D001665:Binding Sites; D001943:Breast Neoplasms; D002460:Cell Line; D006224:Cricetinae; D004958:Estradiol / Q000378:metabolism* / Q000494:pharmacology; D047628:Estrogen Receptor alpha; D005260:Female; D006801:Humans; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D010766:Phosphorylation; D010768:Phosphoserine / Q000378:metabolism; D015221:Potassium Channels / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D024681:Potassium Channels, Calcium-Activated; D017433:Protein Structure, Secondary; D011960:Receptors, Estrogen / Q000737:chemistry / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D051640:Small-Conductance Calcium-Activated Potassium Channels; D014158:Transcription, Genetic; D015533:Transcriptional Activation / Q000502:physiology*; D014407:Tumor Cells, Cultured
D047628:Estrogen Receptor alpha; D015221:Potassium Channels; D024681:Potassium Channels, Calcium-Activated; D011960:Receptors, Estrogen; D011994:Recombinant Proteins; D051640:Small-Conductance Calcium-Activated Potassium Channels; D010768:Phosphoserine; D004958:Estradiol