[Prolongation of MGE 412 transposition induction after gamma-irradiation in an isogenic line of Drosophila melanogaster]].
The dose dependence of the rate of gamma-induced transpositions and consequent dynamics of the MGE 412 pattern after gamma-irradiation were investigated in isogenic line 49 in generations F1, F12, F140, and F170. It was shown that the results on dose dependence of transpositions was very similar with the corresponding results of the classic works by Timofeeff-Ressovsky et al. (1935). It is suggested that the transcribed copies of retrotransposon 412 "cure" gamma-radiation-induced double-strand DNA breaks. The phenomenon of prolongation of MGE transposition induction during early generations after treatment was shown. In this period (F1-F12), the maximum transposition rate (lambda approximately equal to 2 x 10(-2) events per MGE copy, per haploid genome, per generation) and the maximum number of heterozygous MGE copies were achieved. In the late generations (F140 and F170), the reduced induction level (lambda approximately 10(-3) was established. In the population of effective size Ne = 2000 individuals, this corresponds to the state when lambda >> 1/4Ne, i.e., when the transposition flow prevails over the MGE copy loss by genetic drift. These data together with some indirect evidence argue for the hypothesis that the spontaneous transposition rate is proportional to the average number of heterozygous MGE copies per diploid genome.
Ratner
V A
VA
Institute of Cytology and Genetics, Russian Academy of Sciences, Novosibirsk, 630090 Russia. ratner@bionet.nsc.ru
Bubenshchikova
E V
EV
Vasil'eva
L A
LA
D000818:Animals; D004251:DNA Transposable Elements / Q000235:genetics* / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D004331:Drosophila melanogaster / Q000235:genetics* / Q000528:radiation effects; D005720:Gamma Rays; D005786:Gene Expression Regulation / Q000528:radiation effects; D017344:Genes, Insect / Q000528:radiation effects; D016678:Genome; D006579:Heterozygote
D004251:DNA Transposable Elements