A novel phytyltransferase from Synechocystis sp. PCC 6803 involved in tocopherol biosynthesis.
The deduced polypeptide sequence of open reading frame slr1736 reveals homology to chlorophyll synthase and 1,4-dihydroxy-2-naphthoic acid phytyltransferase in Synechocystis sp. strain PCC 6803. In tocopherol and plastoquinone biosynthesis, a condensation reaction mechanistically similar to that of these two enzymes is performed. To analyze the function of this novel prenyltransferase, a deletion mutant of slr1736 was generated by homologous recombination. The mutant showed a markedly decreased tocopherol content, while plastoquinone levels remained unchanged. Since the aromatic precursor homogentisic acid accumulated in the mutant, the function of the enzyme was proven to be a novel tocopherol phytyltransferase.
Schledz
M
M
Greenovation Pflanzenbiotechnologie GmBH, Freiburg, Germany. mschledz@greenovation.com
Seidler
A
A
Beyer
P
P
Neuhaus
G
G
D019883:Alkyl and Aryl Transferases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D003001:Cloning, Molecular; D000458:Cyanobacteria / Q000201:enzymology* / Q000235:genetics; D017353:Gene Deletion; D006713:Homogentisic Acid / Q000378:metabolism; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D010860:Pigments, Biological / Q000378:metabolism; D010971:Plastoquinone / Q000378:metabolism; D016415:Sequence Alignment; D014810:Vitamin E / Q000096:biosynthesis* / Q000378:metabolism
D010860:Pigments, Biological; D014810:Vitamin E; D019883:Alkyl and Aryl Transferases; C412723:phytyltransferase; D006713:Homogentisic Acid; D010971:Plastoquinone