Down-regulation of L-type calcium channel in pups born to 52 kDa SSA/Ro immunized rabbits.
Congenital heart block is considered a model of passively acquired autoimmune disease in which the mother generates anti-SSA/Ro and/or anti-SSB/La antibodies that cross the placenta and presumably injure the heart of developing fetus. CHB is accompanied by ECG abnormalities including AV block, sinus bradycardia, and ventricular dysfunction. Our previous data indicate that these abnormalities are caused by maternal autoantibody-mediated disturbance of L-type Ca channels. To investigate the consequence of chronic exposure of L-type Ca channels in newborn pups to maternal autoantibodies during pregnancy, we immunized female rabbits with human 52 kDa-SSA/Ro (Ro52) recombinant protein. ECG revealed that pups from the immunized group had varying degrees of conduction defects. In addition, I(CaL) density and protein were reduced in hearts of pups from the immunized group. Sera and purified IgG from immunized rabbits inhibited I(Ba) recorded from oocytes with expressed alpha(1C) and beta(2a) subunits of L-type Ca channel. Pups born to Ro52 immunized mothers exhibited down-regulation of L-type calcium channels in heart. The data provide new insight into the pathogenesis of congenital heart block.
Xiao
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Molecular and Cellular Cardiology Program, New York Harbor HealthCare System and SUNY Health Science Center, Brooklyn, New York 11209, USA.
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Hu
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Boutjdir
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D000818:Animals; D001323:Autoantibodies; D001324:Autoantigens / Q000008:administration & dosage* / Q000276:immunology; D020746:Calcium Channels, L-Type / Q000378:metabolism* / Q000502:physiology; D015536:Down-Regulation; D004562:Electrocardiography; D004594:Electrophysiology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006321:Heart / Q000502:physiology; D007114:Immunization; D007074:Immunoglobulin G / Q000276:immunology / Q000494:pharmacology; D008970:Molecular Weight; D009206:Myocardium / Q000378:metabolism; D009865:Oocytes; D020733:RNA, Small Cytoplasmic; D011817:Rabbits; D012261:Ribonucleoproteins / Q000008:administration & dosage* / Q000276:immunology; D014982:Xenopus laevis; D000099207:SS-A Antigen
D001323:Autoantibodies; D001324:Autoantigens; D020746:Calcium Channels, L-Type; D007074:Immunoglobulin G; D020733:RNA, Small Cytoplasmic; D012261:Ribonucleoproteins; D000099207:SS-A Antigen; C116006:RO60 protein, human