Purkinje cells in the adult cat cerebellar cortex possess a perineuronal net of proteoglycans.
The Purkinje cells in the adult cat cerebellar cortex were found to possess perineuronal proteoglycans which could be stained with our fine cationic iron colloid and Fujita's highly concentrated aldehyde fuchsin, and digested by chondroitinase ABC/keratanase/ heparitinase and hyaluronidase. The Purkinje cells are surrounded by some collagenous elements which are stained with Gömöri's ammoniacal silver and digested by collagenase. The Purkinje cells also express nerve cell surface glycoproteins which are labeled with lectin Vicia villosa agglutinin and digested by a double treatment with collagenase and endo-alpha-N-acetylgalactosaminidase. Sole digestion by endo-alpha-N-acetylgalactosaminidase never erased the lectin labeling of the nerve cell surface glycoproteins. These findings suggest that the collagenous elements mediate the linkage of the perineuronal proteoglycans to the nerve cell surface glycoproteins. It is presumed that in mice and rats, the perineuronal nets of proteoglycans and nerve cell surface glycoproteins of the Purkinje cells are so thin or coarse that they can not be sufficiently visualized under the light microscope.
Mabuchi
M
M
Department of Anatomy, Faculty of Medicine, Okayama University Medical School, Japan.
Murakami
S
S
Taguchi
T
T
Ohtsuka
A
A
Murakami
T
T
D000818:Animals; D002415:Cats; D002525:Cerebellar Cortex / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D019765:Chondroitin ABC Lyase; D006026:Glycoside Hydrolases; D006821:Hyaluronoglucosaminidase; D037102:Lectins; D008297:Male; D009474:Neurons / Q000378:metabolism* / Q000648:ultrastructure; D016612:Paraffin Embedding; D011133:Polysaccharide-Lyases; D011509:Proteoglycans / Q000378:metabolism*; D011689:Purkinje Cells / Q000378:metabolism* / Q000648:ultrastructure; D001616:beta-Galactosidase
D037102:Lectins; D011509:Proteoglycans; D006026:Glycoside Hydrolases; C026093:keratan-sulfate endo-1,4-beta-galactosidase; D001616:beta-Galactosidase; D006821:Hyaluronoglucosaminidase; D011133:Polysaccharide-Lyases; D019765:Chondroitin ABC Lyase; C021349:heparitinsulfate lyase