title: Immunosuppressive effects of aflatoxin B1 in Indian major carp (Labeo rohita).
abstract: Aflatoxin B1 (AFB1) is a potent immunomodulator in endotherms. The experiment was carried out to study the immunosuppressive nature of AFB1 in one ectothermic species of Indian major carp. Graded levels (0, 1.25, 5.00 mg/kg of body weight) of purified AFB1 were intraperitoneally (i.p.) injected into rohu (Labeo rohita) fingerlings weighing 30-50 g, and the fish were observed for a period of 90 days. At the end of the trial, blood samples were collected from the control group as well as the AFB1 injected fish and were screened for nitroblue tetrazolium (NBT) assay, serum total protein, albumin, globulin, albumin-globulin ratio (A:G), serum bactericidal activity and bacterial agglutination titre against Edwardsiella tarda. The aflatoxin-treated fish revealed a reduction of total protein, globulin levels, bacterial agglutination titre, NBT and serum bactericidal activities, as well as an enhanced A:G ratio without change in albumin concentration, irrespective of dose levels of toxin treatment, when compared to the control group. Thus, AFB1 proved to be immunosuppressive in rohu even at the lowest dose (1.25 mg/kg body weight) of toxin treatment. This could be of economic significance in intensive culture systems of rohu.
authors: Division of Aquatic Animal Health, Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar, India. cifa@xori.nic.in
authors: P K
authors: PK
authors: Sahoo
authors: S C
authors: SC
authors: Mukherjee
mesh_terms: D016604:Aflatoxin B1 / Q000276:immunology / Q000494:pharmacology*; D000372:Agglutination Tests / Q000662:veterinary; D000818:Animals; D002347:Carps / Q000276:immunology*; D020609:Edwardsiella tarda / Q000254:growth & development; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D007194:India; D007202:Indicators and Reagents / Q000737:chemistry; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology; D009580:Nitroblue Tetrazolium / Q000737:chemistry; D011897:Random Allocation
chemical_list: D007166:Immunosuppressive Agents; D007202:Indicators and Reagents; D009580:Nitroblue Tetrazolium; D016604:Aflatoxin B1