Cytokine expression of skin T-lymphocytes from patients with atopic dermatitis.
We analysed the cytokine profile of skin T cells by establishing 11 T-cell lines from adult patients with moderate-to-severe atopic eczema using T-cell growth factors interleukin-2 and interleukin-4. We compared T-cell lines from lesional skin of atopic dermatitis patients with those from non-atopic skin of patients with other skin diseases, observing that T-cell lines of patients with atopic dermatitis unstimulated cultures expressed a Th1 profile. After stimulation with anti-CD3 and anti-CD28 monoclonal antibodies, the cytokine expression showed rapid initial upregulation of Th2 followed by a Th1 profile. Furthermore, strong upregulation of interleukin-10 was observed after 24 h stimulation. Our findings suggest that skin T-lymphocytes from atopic dermatitis patients seem to consist of a heterogenous population of Th1 and Th2 or Th0 cells and the results for secreted cytokines indicate that T-cell lines from each inflammatory skin disease showed the corresponding disease-specific original cytokine profile.
Higashi
N
N
Department of Dermatology, Marselisborg Hospital, University of Aarhus, Denmark. nhi_ton@hotmail.com
Bang
K
K
Gesser
B
B
Lund
M
M
Thestrup-Pedersen
K
K
D000328:Adult; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D001483:Base Sequence; D015415:Biomarkers / Q000032:analysis; D001707:Biopsy, Needle; D002478:Cells, Cultured; D016207:Cytokines / Q000032:analysis / Q000378:metabolism*; D003876:Dermatitis, Atopic / Q000276:immunology* / Q000473:pathology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D011336:Probability; D012333:RNA, Messenger / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D012867:Skin / Q000166:cytology; D018709:Statistics, Nonparametric; D018417:Th1 Cells / Q000187:drug effects / Q000276:immunology*; D018418:Th2 Cells / Q000187:drug effects / Q000276:immunology*
D000911:Antibodies, Monoclonal; D015415:Biomarkers; D016207:Cytokines; D012333:RNA, Messenger