Purification and characterization of a natural agglutinin in the hemolymph of the prawn Penaeus indicus H. Milne Edwards.
A natural agglutinin in the hemolymph of the marine prawn Penaeus indicus was isolated by gel filtration chromatography, purified using polyacrylamide gel electrophoresis, and characterized. Prawn agglutinin has a native molecular mass of 181 kDa and consists of two monomeric units (97 and 84 kDa), maintains some agglutinating activity over a wide pH range (7-9), and is inactivated at 85 degrees C. The agglutinin was denatured upon mixing with trichloroacetic acid, phenol, chloroform, and 45% ammonium sulfate. It was also sensitive to trypsin digestion. The results indicate that prawn agglutinin is proteinaceous in nature, with agglutinating, hemolytic, and antibacterial properties against marine bacteria and erythrocytes with carbohydrate binding sites.
Jayasree
S
S
Biochemistry and Genetic Engineering Research Unit, Cochin University of Science and Technology, Cochin, 682 022, India. tkd@kfri.org
D000373:Agglutinins / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification / Q000502:physiology; D000818:Animals; D002850:Chromatography, Gel / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006458:Hemolymph / Q000276:immunology*; D006863:Hydrogen-Ion Concentration; D008297:Male; D033561:Penaeidae / Q000276:immunology*; D012967:Sodium Dodecyl Sulfate
D000373:Agglutinins; D012967:Sodium Dodecyl Sulfate