title: Potential impact of the VITEK 2 system and the Advanced Expert System on the clinical laboratory of a university-based hospital.
abstract: A study was designed to assess the impact of the VITEK 2 automated system and the Advanced Expert System (AES) on the clinical laboratory of a typical university-based hospital. A total of 259 consecutive, nonduplicate isolates of Enterobacteriaceae members, Pseudomonas aeruginosa, and Staphylococcus aureus were collected and tested by the VITEK 2 system for identification and antimicrobial susceptibility testing, and the results were analyzed by the AES. The results were also analyzed by a human expert and compared to the AES analyses. Among the 259 isolates included in this study, 245 (94.6%) were definitively identified by VITEK 2, requiring little input from laboratory staff. For 194 (74.9%) isolates, no inconsistencies between the identification of the strain and the antimicrobial susceptibility determined by VITEK 2 were detected by the AES. Thus, no input from laboratory staff was required for these strains. The AES suggested one or more corrections to results obtained with 65 strains to remove inconsistencies. The human expert thought that most of these corrections were appropriate and that some resulted from a failure of the VITEK 2 system to detect certain forms of resistance. Antimicrobial phenotypes assigned to the strains by the AES for beta-lactams, aminoglycosides, quinolones, macrolides, tetracyclines, and glycopeptides were similar to those assigned by the human expert for 95.7 to 100% of strains. These results indicate that the VITEK 2 system and AES can provide accurate information in tests for most of the clinical isolates examined and remove the need for human analysis of results for many. Certain problems were identified in the study that should be remediable with further work on the software supporting the AES.
authors: Center for Research in Anti-Infectives and Biotechnology, Department of Medical Microbiology and Immunology, Creighton University School of Medicine, Omaha, Nebraska 68178, USA.
authors: C C
authors: CC
authors: Sanders
authors: M
authors: M
authors: Peyret
authors: E S
authors: ES
authors: Moland
authors: S J
authors: SJ
authors: Cavalieri
authors: C
authors: C
authors: Shubert
authors: K S
authors: KS
authors: Thomson
authors: J M
authors: JM
authors: Boeufgras
authors: W E
authors: WE
authors: Sanders
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D015373:Bacterial Typing Techniques / Q000295:instrumentation / Q000379:methods; D004352:Drug Resistance, Microbial; D004755:Enterobacteriaceae / Q000145:classification / Q000187:drug effects; D005103:Expert Systems; D006785:Hospitals, University; D006801:Humans; D007755:Laboratories, Hospital; D017056:Medical Laboratory Personnel; D008826:Microbial Sensitivity Tests / Q000295:instrumentation / Q000379:methods; D011550:Pseudomonas aeruginosa / Q000145:classification / Q000187:drug effects; D011933:Reagent Kits, Diagnostic; D013211:Staphylococcus aureus / Q000145:classification / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D011933:Reagent Kits, Diagnostic