title: Creation of chimeric mutant axolotls: a model to study early embryonic heart development in Mexican axolotls.
abstract: The Mexican axolotl (Ambystoma mexicanum) provides an excellent model for studying heart development since it carries a cardiac lethal mutation in gene c that results in failure of contraction of mutant embryonic myocardium. In cardiac mutant axolotls (c/c) the hearts do not beat, apparently because of an absence of organized myofibrils. To date, there has been no way to analyze the genotypes of embryos from heterozygous spawnings (+/c x +/c) until stage 35 when the normal (+/c or +/+) embryos first begin to have beating hearts; mutant (c/c) embryos fail to develop normal heartbeats. In the present study, we created chimeric axolotls by using microsurgical techniques. The general approach was to transect tailbud embryos and join the anterior and posterior halves of two different individuals. The chimeric axolotl is composed of a normal head and heart region (+/+), permitting survival and a mutant body containing mutant gonads (c/c) that permits the production of c/c mutant offspring: 100% c/c offspring were obtained by mating c/c chimeras (c/c x c/c). The mutant phenotypes were confirmed by the absence of beating hearts and death at stage 41 in 100% of the embryos. Examination of the mutant hearts with electron microscopy and comfocal microscopy after immunofluorescent staining for tropomyosin showed identical images to those described previously in naturally-occurring c/c mutant axolotls (i.e., lacking organized sarcomeric myofibrils). These "c/c chimeric" axolotls provide a useful and unique way to investigate early embryonic heart development in cardiac mutant Mexican axolotls.
authors: Department of Medical Physiology, TX A&M University System Health Science Center, 108 Reynolds Medical Building, College Station, Texas 77843, USA. l-lemanski@tamu.edu
authors: L F
authors: LF
authors: Lemanski
authors: F
authors: F
authors: Meng
authors: S L
authors: SL
authors: Lemanski
authors: N
authors: N
authors: Dawson
authors: C
authors: C
authors: Zhang
authors: D
authors: D
authors: Foster
authors: Q
authors: Q
authors: Li
authors: M
authors: M
authors: Nakatsugawa
authors: R W
authors: RW
authors: Zajdel
authors: D K
authors: DK
authors: Dube
authors: X
authors: X
authors: Huang
mesh_terms: D000557:Ambystoma / Q000196:embryology* / Q000235:genetics*; D000818:Animals; D002678:Chimera; D005260:Female; D005838:Genotype; D006321:Heart / Q000196:embryology*; D008297:Male; D018613:Microscopy, Confocal; D008854:Microscopy, Electron; D023421:Models, Animal; D009154:Mutation; D009206:Myocardium / Q000648:ultrastructure; D010641:Phenotype; D013997:Time Factors