title: Antagonistic forces generated by myosin II and cytoplasmic dynein regulate microtubule turnover, movement, and organization in interphase cells.
abstract: Photoactivation of caged fluorescent tubulin was used mark the microtubule (MT) lattice and monitor MT behavior in interphase cells. A broadening of the photoactivated region occurred as MTs moved bidirectionally. MT movement was not inhibited when MT assembly was suppressed with nocodazole or Taxol; MT movement was suppressed by inhibition of myosin light chain kinase with ML7 or by a peptide inhibitor. Conversely, MT movement was increased after inhibition of cytoplasmic dynein with the antibody 70.1. In addition, the half-time for MT turnover was decreased in cells treated with ML7. These results demonstrate that myosin II and cytoplasmic dynein contribute to a balance of forces that regulates MT organization, movement, and turnover in interphase cells.
authors: Program in Molecular and Cellular Biology and Department of Biology, University of Massachusetts, Amherst, MA 01003, USA.
authors: A M
authors: AM
authors: Yvon
authors: D J
authors: DJ
authors: Gross
authors: P
authors: P
authors: Wadsworth
mesh_terms: D000205:Actomyosin / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D003593:Cytoplasm / Q000378:metabolism; D004398:Dyneins / Q000378:metabolism*; D007399:Interphase; D016547:Kinesins / Q000378:metabolism; D008870:Microtubules / Q000378:metabolism / Q000502:physiology*; D008969:Molecular Sequence Data; D009218:Myosins / Q000378:metabolism*; D014404:Tubulin / Q000378:metabolism
chemical_list: D014404:Tubulin; D000205:Actomyosin; D009218:Myosins; D004398:Dyneins; D016547:Kinesins