title: Proliferation and apoptosis in the epithelium of the developing human cornea and conjunctiva.
abstract: To determine the distribution of proliferating and apoptotic cells in the human cornea during prenatal and early postnatal development, we examined sections of the bulbar conjunctiva, the limbus as well as the central and peripheral cornea between 11 weeks of gestation and 6 months after birth. The objective was to localize dividing cells by proliferating cell nuclear antigen-like immunoreactivity (PCNA-LI) and apoptotic cells by terminal transferase-mediated nick-end labeling (TUNEL). Before the 17th gestational week, PCNA-LI was absent in all 4 regions examined, indicating negligible cell proliferation during early development. After 20 weeks, strong PCNA-labeling was observed in all regions examined suggestive of high proliferative activity not only in the limbus and the bulbar conjunctiva, but also in the central and peripheral cornea. This rise in proliferative activity was followed by a steady decline: after 28 weeks, anti-PCNA staining gradually disappeared in the central and peripheral cornea, so that, at 6 months after birth, it was confined to the limbus and the bulbar conjunctiva, resembling the picture described for the adult cornea. TUNEL-positive cells were virtually absent in all 4 regions examined before the 38th gestational week. Apoptotic cells only started to appear at 38 weeks; at this stage, they were confined to the bulbar conjunctival epithelium. At 6 months after birth, TUNEL-positive cells were observed in the bulbar conjunctival epithelium and the entire cornea; the limbus, however remained devoid of apoptotic cells throughout the entire prenatal and early postnatal period. The present study for the first time localizes proliferating and apoptotic cells in the epithelium of the developing human cornea. Three stages of development can be distinguished: Minimal proliferation (until 17th week), vigorous proliferation over the entire cornea including the limbus and the bulbar conjunctiva (until 28th week) and gradual decrease in proliferative activity (after 28th week) accompanied by the appearance of apoptotic cells.
authors: Department of Anatomy, The Chinese University of Hong Kong, Shatin, New Territories. b175750@mailserv.cuhk.edu.hk
authors: D T
authors: DT
authors: Yew
authors: O
authors: O
authors: Sha
authors: W W
authors: WW
authors: Li
authors: T T
authors: TT
authors: Lam
authors: D E
authors: DE
authors: Lorke
mesh_terms: D000375:Aging / Q000502:physiology*; D017209:Apoptosis / Q000502:physiology*; D002455:Cell Division; D003228:Conjunctiva / Q000166:cytology* / Q000196:embryology / Q000254:growth & development / Q000502:physiology; D005314:Embryonic and Fetal Development / Q000502:physiology*; D004847:Epithelial Cells / Q000166:cytology* / Q000502:physiology; D019573:Epithelium, Corneal / Q000166:cytology* / Q000196:embryology / Q000254:growth & development; D005865:Gestational Age; D006801:Humans; D020287:In Situ Nick-End Labeling; D007223:Infant; D007231:Infant, Newborn; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis
chemical_list: D018809:Proliferating Cell Nuclear Antigen