title: Accelerated tissue aging and increased oxidative stress in broiler chickens fed allopurinol.
abstract: Uric acid has been hypothesized as being one of the more important antioxidants in limiting the accumulation of glycosylated endproducts in birds. Study 1 was designed to quantitatively manipulate the plasma concentrations of uric acid using hemin and allopurinol while study 2 determined their effects on skin pentosidine, the shear force value of Pectoralis major muscle, plasma glucose, body weight and chemiluminescence monitored oxidative stress in broiler chickens. Hemin was hypothesized to raise uric acid concentrations thereby lowering oxidative stress whereas allopurinol was hypothesized to lower uric acid concentrations and raise measures of oxidative stress. In study 1 feeding allopurinol (10 mg/kg body weight) to 8-week-old broiler chicks (n=50) for 10 days decreased plasma uric acid by 57%. However, hemin (10 mg/kg body weight) increased uric acid concentrations 20%. In study 2, 12-week-old broiler chicks (n=90) were randomly assigned to either an ad libitum (AL) diet or a diet restricted (DR) group. Each group was further divided into three treatments (control, allopurinol or hemin fed). Unexpectedly, hemin did not significantly effect uric acid concentrations but increased (P<0.05) measures of chemiluminescence dependent oxidative stress in both the DR and AL birds probably due to the ability of iron to generate oxygen radicals. Allopurinol lowered concentrations of uric acid and increased (P<0.05) the oxidative stress in the AL birds at week 22, reduced (P<0.05) body weight in both the AL and DR fed birds at 16 and 22 weeks of age, and markedly increased (P<0.001) shear force values of the pectoralis major muscle. Skin pentosidine levels increased (P<0.05) in AL birds fed allopurinol or hemin fed birds, but not in the diet restricted birds at 22 weeks. The significance of these studies is that concentrations of plasma uric acid can be related to measures of oxidative stress, which can be linked to tissue aging.
authors: Division of Animal & Veterinary Sciences, West Virginia University, 26506-6108, Morgantown, WV, USA. hkland@wvu.edu
authors: H
authors: H
authors: Klandorf
authors: D S
authors: DS
authors: Rathore
authors: M
authors: M
authors: Iqbal
authors: X
authors: X
authors: Shi
authors: K
authors: K
authors: Van Dyke
mesh_terms: D000375:Aging / Q000378:metabolism*; D000493:Allopurinol / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D000963:Antimetabolites / Q000494:pharmacology; D001120:Arginine / Q000031:analogs & derivatives* / Q000494:pharmacology*; D001786:Blood Glucose / Q000032:analysis / Q000187:drug effects; D001835:Body Weight / Q000187:drug effects; D002645:Chickens / Q000097:blood / Q000378:metabolism*; D003432:Cross-Linking Reagents / Q000494:pharmacology; D004032:Diet; D017127:Glycation End Products, Advanced / Q000378:metabolism*; D006031:Glycosylation; D006427:Hemin / Q000008:administration & dosage / Q000494:pharmacology*; D008163:Luminescent Measurements; D008239:Lysine / Q000031:analogs & derivatives* / Q000494:pharmacology*; D018482:Muscle, Skeletal / Q000187:drug effects; D018384:Oxidative Stress; D012867:Skin / Q000378:metabolism; D014527:Uric Acid / Q000097:blood / Q000378:metabolism*
chemical_list: D000963:Antimetabolites; D001786:Blood Glucose; D003432:Cross-Linking Reagents; D017127:Glycation End Products, Advanced; D014527:Uric Acid; D000493:Allopurinol; D006427:Hemin; D001120:Arginine; C062187:pentosidine; D008239:Lysine