title: [Study on frequencies of aneuploidy in mouse oocyte and female pronucleus of one cell zygote induced by benzene].
abstract: OBJECTIVE
  To study the effect of benzene on aneupoidy of oocytes and female pronucleus of one cell zygotes.
  
  
  METHODS
  The NIH adult female mice were treated with varies doses of benzene (942, 1,881 and 3,762 mg/kg respectively) via single gavage and (706, 1,922 and 4,864 mg/m3, respectively) multiple inhalation. After gavage, the mice were pair-matched (1:1) with males overnight. The ovulated oocytes and the one cell zygotes were collected for cytogenetic analysis and the frequencies of aneuploidy were detected.
  
  
  RESULTS
  The frequencies of aneuploid in M II oocytes significantly increased in the three groups treated with inhalation (7.06%, 7.50% and 7.76%, respectively, control group 0.00%, P < 0.05) of benzene, as compared with those in the control group (1.30%), with a dose-dependent response. Meiotic delay of M I oocytes was observed, with the frequency of M I oocytes of 1.16%, 3.61% and 5.75%, respectively, P < 0.05, in a dose-dependent response, too. In gavage treatment group, increase in frequency of M II oocyte aneuploidy was only observed in the high-dose group, and aneupliody frequency of female pronucleus in one cell zygotes was not induced.
  
  
  CONCLUSIONS
  Exposure to high-dose benzene via gavage or inhalation was able to induce increase in aneuploidy frequency of M II oocytes in mice.
authors: Department of Industrial Hygiene and Oocupational Diseases, School of Public Health, Sun Yat-Sen University of Medical Sciences, Guangzhou 510089, China.
authors: Q
authors: Q
authors: Zeng
authors: L
authors: L
authors: Zheng
authors: L
authors: L
authors: Deng
mesh_terms: D000782:Aneuploidy; D000818:Animals; D001554:Benzene / Q000633:toxicity*; D005260:Female; D051379:Mice; D009865:Oocytes / Q000187:drug effects* / Q000473:pathology; D015053:Zygote / Q000187:drug effects* / Q000473:pathology
chemical_list: D001554:Benzene