title: Diagnostic investigation of chronic porcine reproductive and respiratory syndrome virus in a breeding herd of pigs.
abstract: Forty-five sows and 15 boars were selected at random from a breeding herd known to be chronically infected with porcine reproductive and respiratory syndrome virus (PRRSV) and lymphoid, immune-privileged, and non-lymphoid/non-immune-privileged tissues were tested for the presence of the virus by PCR, virus isolation, and immunohistochemistry. The virus was isolated from the lateral retropharyngeal lymph node of one sow; the isolate was nucleic acid sequenced and determined to be of field origin, and it was inoculated into two PRRSV-naive pregnant sows (A and B) at 95 days of gestation. They were necropsied 14 days later and samples of maternal and fetal tissue and blood samples were collected. Sow A had 10 fresh, six partially autolysed, and two mummified fetuses, and sow B had six fresh and viable fetuses. Viral nucleic acid was detected by PCR in tissue pools from each sow and also from pooled fetal tissues, and the virus was isolated from fetal pools from sow A.
authors: Department of Clinical and Population Sciences, University of Minnesota College of Veterinary Medicine, St Paul 55108, USA.
authors: M D
authors: MD
authors: Bierk
authors: S A
authors: SA
authors: Dee
authors: K D
authors: KD
authors: Rossow
authors: J E
authors: JE
authors: Collins
authors: M I
authors: MI
authors: Guedes
authors: C
authors: C
authors: Pijoan
authors: T W
authors: TW
authors: Molitor
mesh_terms: D000818:Animals; D002908:Chronic Disease; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D008297:Male; D016133:Polymerase Chain Reaction; D019318:Porcine Reproductive and Respiratory Syndrome / Q000175:diagnosis* / Q000821:virology; D019316:Porcine respiratory and reproductive syndrome virus / Q000302:isolation & purification* / Q000472:pathogenicity; D013552:Swine