Theileria annulata: identification, by differential mRNA display, of modulated host and parasite gene expression in cell lines that are competent or attenuated for differentiation to the merozoite.
To identify both host and parasite genes that show altered expression during differentiation of Theileria annulata from the macroschizont to the merozoite stage of the life cycle, the RNA profiles of two T. annulata-infected clonal cell lines (D7 and D7B12) with the same genetic background have been compared by RNA display. In the cloned cell line D7, T. annulata differentiates from the macroschizont to the merozoite at 41 degrees C, whereas in the cell line D7B12, which was derived by recloning D7, the parasite does not differentiate. Therefore, genes that show altered expression levels in either clone could be modulated by the differentiation event and are possible candidates for regulators of this process. Differential display was carried out initially on RNA extracted from D7 and D7B12 macroschizont-infected cells cultured at 37 degrees C and secondly on RNA extracted from the two cell lines incubated at 41 degrees C to induce differentiation to the merozoite. The first procedure identified 29 cDNA fragments that displayed altered levels between D7 and D7B12, 9 of which were confirmed to be differentially expressed by Northern blot analysis. Of these 9 gene fragments, 8 were found to be of host origin, while 1 was parasite derived. The second RNA display analysis identified 14 transcripts that showed altered levels during a differentiation time course, of which 6 were confirmed to be differentially expressed between D7B12 cells and differentiating D7 cells by Northern blot analysis. Of these 6 gene fragments, 1 was of host and 5 were of parasite origin. The parasite genes either showed levels of RNA consistent with constitutive gene expression or, in one case, a genuine upregulation of mRNA associated with the differentiation process.
Oura
C A
CA
Department of Veterinary Parasitology, Glasgow University, Bearsden Road, Glasgow, G61 1QH, Scotland. c.oura@vet.gla.ac.uk
Tait
A
A
Shiels
B R
BR
D000818:Animals; D015152:Blotting, Northern; D015139:Blotting, Southern; D002417:Cattle; D002460:Cell Line; D017931:DNA Primers; D018076:DNA, Complementary / Q000032:analysis; D018507:Gene Expression Regulation, Developmental; D012333:RNA, Messenger / Q000032:analysis; D016796:Theileria annulata / Q000235:genetics* / Q000254:growth & development
D017931:DNA Primers; D018076:DNA, Complementary; D012333:RNA, Messenger