Cytoprotective role of Solanum nigrum against gentamicin-induced kidney cell (Vero cells) damage in vitro.
The 50% ethanol extract of the whole plant of Solanum nigrum was tested in vitro for its cytoprotection against gentamicin-induced toxicity on Vero cells. Cytotoxicity was significantly inhibited as assessed by the Trypan blue exclusion assay and mitochondrial dehydrogenase activity (MTT) assay. The test extract also exhibited significant hydroxyl radical scavenging potential, thus suggesting its probable mechanism of cytoprotection.
Prashanth Kumar
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Department of Pharmacognosy, Government College of Pharmacy, Bangalore-5600 27, India.
Shashidhara
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S
Kumar
M M
MM
Sridhara
B Y
BY
D000818:Animals; D002522:Chlorocebus aethiops; D016166:Free Radical Scavengers / Q000494:pharmacology*; D005839:Gentamicins; D006801:Humans; D007668:Kidney / Q000378:metabolism; D008928:Mitochondria / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology; D010946:Plants, Medicinal; D020011:Protective Agents / Q000494:pharmacology*; D019657:Solanaceae; D014343:Trypan Blue; D014709:Vero Cells / Q000187:drug effects
D016166:Free Radical Scavengers; D005839:Gentamicins; D010936:Plant Extracts; D020011:Protective Agents; D014343:Trypan Blue