Branching projections of catecholaminergic ventrolateral reticular neurons to the fastigial nucleus and superior colliculus in the rat: triple labelling procedure.
In this study, we employed triple fluorescent-labelling to reveal the distribution of the catecholaminergic neurons within rostral ventrolateral reticular nucleus which supply branching collateral input to the superior colliculus (SC) and to the cerebellar fastigial nucleus (FN). The catecholaminergic identity of the neurons was revealed by immunocytochemical detection of the biosynthetic enzyme, tyrosine hydroxylase. The projections were defined by injections of two retrograde tracers: rhodamine and fluoro gold in the SC and FN, respectively.
Stanzani
S
S
Department of Physiological Sciences; University of Catania, Viale A. Doria, 6-95125, Catania, Italy.
Russo
A
A
Pellitteri
R
R
Storaci
G
G
Cataudella
T
T
D000818:Animals; D001369:Axons / Q000378:metabolism / Q000648:ultrastructure*; D002395:Catecholamines / Q000378:metabolism*; D002529:Cerebellar Nuclei / Q000166:cytology* / Q000378:metabolism; D005456:Fluorescent Dyes / Q000494:pharmacology; D007150:Immunohistochemistry; D008297:Male; D008526:Medulla Oblongata / Q000166:cytology* / Q000378:metabolism; D008863:Microspheres; D009434:Neural Pathways / Q000166:cytology* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012154:Reticular Formation / Q000166:cytology* / Q000378:metabolism; D013477:Superior Colliculi / Q000166:cytology* / Q000378:metabolism; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
D002395:Catecholamines; D005456:Fluorescent Dyes; D014446:Tyrosine 3-Monooxygenase