Uniformly (14)C-ring-labeled 2,4-dichlorophenoxyacetic acid: a metabolism study in bluegill sunfish, Lepomis macrochirus.
The fate of 2,4-dichlorophenoxyacetic acid (2,4-D), a mixture of [phenyl(U)-(14)C]-2,4-D and unlabeled 2,4-D, in bluegill sunfish was investigated after exposure to approximately 11 ppm under static conditions for 4 days. Total radioactive residues (TRR) in whole fish increased from 0.41 ppm on day 1 to 0.60 ppm on day 3. TRR levels in fillet (edible) and viscera (nonedible) of treated fish on day 4 were 0.41 and 1.9 ppm, respectively. Most residues in both matrices were acetonitrile soluble; small amounts were hexane soluble or unextractable with solvents. Acid and base hydrolyses with ethyl acetate partitioning were used to release the fillet unextractable residues. The identification of 2,4-D and 2,4-dichlorophenol (2,4-DCP) in the fillet was conclusively confirmed by GC-MS analysis. On the basis of the experimental data from this study, a metabolic pathway for 2,4-D in bluegill sunfish in which the 2,4-D is metabolized to 2,4-DCP and conjugates of 2,4-D and 2,4-DCP is proposed.
Barnekow
D E
DE
ABC Laboratories, Inc., 7200 East ABC Lane, Columbia, Missouri 65205, USA. debarnekow@dowagro.com
Premkumar
N D
ND
Stewart
S
S
Hamburg
A W
AW
D015084:2,4-Dichlorophenoxyacetic Acid / Q000378:metabolism*; D000818:Animals; D002247:Carbon Isotopes; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D010473:Perciformes / Q000378:metabolism*; D010573:Pesticide Residues / Q000032:analysis*; D014018:Tissue Distribution
D002247:Carbon Isotopes; D010573:Pesticide Residues; D015084:2,4-Dichlorophenoxyacetic Acid