Mutants of tobacco mosaic virus with temperature-sensitive coat proteins induce heat shock response in tobacco leaves.
We analyzed, with respect to heat shock proteins (HSPs), systemically reacting tobacco leaves inoculated with Tobacco mosaic virus (TMV), wild-type vulgare, and temperature-sensitive coat protein (CP) mutants Ni 118 (P20L) and flavum (D19A), kept at 23 or 30 degrees C. HSP18 and HSP70 mRNAs and proteins were induced with temperature-sensitive CP mutants after 1 to 2 days at 30 degrees C. After 4 to 6 days, HSP70 was also induced at 23 degrees C. The induction of HSPs paralleled the amount of insoluble TMV CP in leaf extracts, indicating that denatured TMV CP by itself induces a heat-shock response.
Jockusch
H
H
Developmental Biology and Molecular Pathology, University of Bielefeld, Germany. h.jockusch@biologie.uni-bielefeld.de
Wiegand
C
C
Mersch
B
B
Rajes
D
D
D002213:Capsid / Q000235:genetics* / Q000502:physiology; D005814:Genes, Viral; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis / Q000235:genetics; D006360:Heat-Shock Proteins / Q000096:biosynthesis / Q000235:genetics; D018869:Heat-Shock Response; D009154:Mutation; D010935:Plant Diseases / Q000821:virology; D018515:Plant Leaves / Q000378:metabolism / Q000821:virology; D010940:Plant Proteins; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D013696:Temperature; D014026:Nicotiana / Q000235:genetics / Q000502:physiology / Q000821:virology*; D014027:Tobacco Mosaic Virus / Q000235:genetics* / Q000472:pathogenicity*
C489905:HSP18 protein, plant; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D010940:Plant Proteins; D012333:RNA, Messenger; D018749:RNA, Plant