The MgtE Mg2+ transport protein is involved in Aeromonas hydrophila adherence.
Aeromonas hydrophila AH-3 strains carrying mutations in mgtE, which encodes a Mg2+ and Co2+ transport system, showed a 50% reduction of in vitro adherence to HEp-2 cells, a reduction in swarming in semisolid swarming agar, and decrease in biofilm formation of over 60% in comparison to the wild-type strain. The cloned A. hydrophila mgtE expressed from a plasmid complements a Salmonella typhimurium strain deleted for all Mg2+ transporters both phenotypically and by measurement of 57Co2+ uptake. Likewise, plasmid-borne mgtE was able to complement the changes observed in A. hydrophila mgtE mutants. We suggest that MgtE and thus Mg2+ and possibly Co2+ have a role in A. hydrophila related to their swarming ability and related consequences such as adherence and biofilm formation.
Merino
S
S
Departamento Microbiologia, Facultad Biología, Universidad de Barcelona, Spain.
Gavín
R
R
Altarriba
M
M
Izquierdo
L
L
Maguire
M E
ME
Tomás
J M
JM
D016980:Aeromonas hydrophila / Q000235:genetics / Q000502:physiology*; D017920:Antiporters / Q000235:genetics / Q000378:metabolism*; D001422:Bacterial Adhesion / Q000502:physiology*; D001426:Bacterial Proteins / Q000378:metabolism; D018441:Biofilms; D001692:Biological Transport; D003001:Cloning, Molecular; D003035:Cobalt / Q000378:metabolism; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000502:physiology; D005816:Genetic Complementation Test; D006801:Humans; D008274:Magnesium / Q000378:metabolism*; D009068:Movement / Q000502:physiology; D009154:Mutation; D015183:Restriction Mapping; D012486:Salmonella typhimurium / Q000235:genetics / Q000502:physiology; D017422:Sequence Analysis, DNA; D014407:Tumor Cells, Cultured
D017920:Antiporters; D001426:Bacterial Proteins; D004269:DNA, Bacterial; C092192:MgtE protein, bacteria; D003035:Cobalt; D008274:Magnesium