title: Detection of mutant k-ras sequences in the urine of cancer patients.
abstract: DNA fragments from apoptotic cells crossing the renal barrier retain their matrix functions, which allows PCR identification of mutant sequences in excreted DNA. We investigated the possibility of detecting k-ras mutations in urinary DNA of tumor patients (colon cancer). In some patients with k-ras codon 12 mutations in tumor cell DNA the same changes were detected in the urinary DNA. The possibility of using this approach for early diagnosis and monitoring of tumors is discussed.
authors: N. N. Blokhin National Cancer Research Center, Moscow.
authors: O I
authors: OI
authors: Serdyuk
authors: I V
authors: IV
authors: Botezatu
authors: V P
authors: VP
authors: Shelepov
authors: G I
authors: GI
authors: Potapova
authors: R P
authors: RP
authors: Alekhina
authors: Y K
authors: YK
authors: Molyaka
authors: V S
authors: VS
authors: Anan'ev
authors: V I
authors: VI
authors: Knysh
authors: O S
authors: OS
authors: Melkonyan
authors: S R
authors: SR
authors: Umanskii
authors: A V
authors: AV
authors: Lichtenshtein
mesh_terms: D003062:Codon; D003110:Colonic Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000652:urine*; D011905:Genes, ras; D006801:Humans; D009154:Mutation; D016133:Polymerase Chain Reaction
chemical_list: D003062:Codon