title: Gastrointestinal transit and anorectal manometry in children with colonic substance P deficiency.
abstract: OBJECTIVE
  Severe intractable constipation in children may be associated with a reduction of substance P (SP)- containing fibers in colonic circular muscle. The aim of this study was to characterize gastrointestinal transit (GIT), anorectal manometry (ARM) and electromyographic (EMG) changes in these children.
  
  
  METHODS
  Seromuscular laparoscopic biopsies of the colon were obtained from 35 children with severe constipation. Immunofluorescent staining for SP and vasoactive intestinal peptide (VIP) were then performed on these specimens. The cohort of patients studied included a SP-deficient group (SPD, n = 25) who had reduced numbers of SP-immunoreactive nerve fibers. The other group consisted of patients with normal staining for both SP and VIP (SPN, n = 10). Gastrointestinal transit studies (gastric emptying, orocecal and colonic transit) suitable for analysis were available for 17 patients (SPD, n = 9 and SPN, n = 8). The colon was divided into segments and radioactivity counts in each segment were expressed as a percentage of the total colonic count at each time point (6, 24, 32 and 48 h). The geometric center (GC), ARM, EMG, clinical and demographic data characteristics of both groups of patients were compared.
  
  
  RESULTS
  There were no differences in demographic data, gastric emptying, orocecal transit or geometric center of transit in the colon between the two patient groups. The ARM and EMG studies suggested that the SPN group have a higher mean threshold volume of balloon distension required to initiate a rectoanal inhibitory reflex, and a higher incidence of anismus; however, this did not reach statistical significance.
  
  
  CONCLUSIONS
  These data suggest a trend that the SPN patients have a greater problem with obstructive defecation and abnormal rectal sensation than those with SPD. We were unable to confirm any defect in colonic transit in the SPD patients compared with the SPN group.
authors: Departments of Gastroenterology and Clinical Nutrition, Royal Children's Hospital, Parkville, Victoria, Australia.
authors: S
authors: S
authors: Treepongkaruna
authors: J M
authors: JM
authors: Hutson
authors: J
authors: J
authors: Hughes
authors: D
authors: D
authors: Cook
authors: A G
authors: AG
authors: Catto-Smith
authors: C W
authors: CW
authors: Chow
authors: M R
authors: MR
authors: Oliver
mesh_terms: D001003:Anal Canal / Q000503:physiopathology; D002648:Child; D002675:Child, Preschool; D003106:Colon / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D003248:Constipation / Q000209:etiology*; D004576:Electromyography; D005260:Female; D005455:Fluorescent Antibody Technique; D005772:Gastrointestinal Transit; D006801:Humans; D007223:Infant; D008297:Male; D008365:Manometry; D012189:Retrospective Studies; D013373:Substance P / Q000172:deficiency* / Q000378:metabolism; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism
chemical_list: D013373:Substance P; D014660:Vasoactive Intestinal Peptide