title: Styrene-induced hearing loss: a membrane insult.
abstract: Styrene is an aromatic solvent widely used as a precursor for polystyrene plastics in many factories which produce glass-reinforced plastic. This solvent has been shown to disrupt the auditory system in both humans and animals. In order to study the sequence of events which could explain the cochlear impairments, a time course experiment was carried out with 6-month-old rats. Male Long Evans rats were exposed to 1000 ppm styrene for 6 h/day, 5 days/week, for either 1, 2, 3, or 4 consecutive weeks. Auditory function was tested by recording the near field evoked potentials from the inferior colliculus, and histological analyses of the cochleae were performed with light and transmission electron microscopy. The electrophysiological results support a toxic mid-frequency process which keeps worsening even after the end of the exposure. The histological findings demonstrate that supporting cells are the first targets of the solvent. Then, the outer hair cells of the third row (OHC3) are disrupted, followed successively by OHC2 and OHC1 from the basal (20 kHz) to the upper turn (4 kHz) of the cochlea. Basically, the disorganization of the membranous structures could be the starting point for the cochlear injury induced by styrene. This paper presents a hypothesis that the accumulation of K+ in the spaces of Nuel underlies the toxic effects of styrene.
authors: Institut National de Recherche et de Sécurité, Laboratoire de Neurotoxicité, Vandoeuvre, France. campo@inrs.fr
authors: P
authors: P
authors: Campo
authors: R
authors: R
authors: Lataye
authors: G
authors: G
authors: Loquet
authors: P
authors: P
authors: Bonnet
mesh_terms: D000818:Animals; D001300:Audiometry, Evoked Response; D003051:Cochlea / Q000187:drug effects / Q000473:pathology; D003638:Deafness / Q000139:chemically induced* / Q000473:pathology / Q000503:physiopathology; D005072:Evoked Potentials, Auditory / Q000187:drug effects; D018072:Hair Cells, Auditory, Outer / Q000187:drug effects / Q000473:pathology; D006801:Humans; D008297:Male; D008854:Microscopy, Electron; D051381:Rats; D012997:Solvents / Q000633:toxicity; D020058:Styrene / Q000633:toxicity*
chemical_list: D012997:Solvents; D020058:Styrene