Expression and function of the Drosophila melanogaster ADH in male Ceratitis capitata adults: a potential strategy for medfly genetic sexing based on gene-transfer technology.
The aim of development of a Mediterranean fruit fly Ceratitis capitata genetic sexing strain derives from the large scale SIT programmes being carried out to control this pest. Toward this direction, we present here the male-specific expression of the Drosophila melanogaster alcohol dehydrogenase (ADH) in medfly transgenic adults generated by Minos-mediated germ line transformation. This expression pattern is obtained by using a promoter fragment of the male-specific gene MSSP-alpha2 of the medfly. We show that the heterologous enzyme is functional in the medfly oxidizing both ethanol and 2-propanol. Although leading to an approximately twofold increase of total ADH activity in male compared to female transgenic adults, these expression levels are not enough for performing genetic sexing when high doses of environmental alcohol are applied. This could be achieved either by further enhancement of the transgene expression or by generating an Adh- line to host the Minos insertions.
Christophides
G K
GK
Department of Genetics and Biotechnology, School of Biological Sciences, University of Athens, Athens, Greece.
Savakis
C
C
Mintzas
A C
AC
Komitopoulou
K
K
D000426:Alcohol Dehydrogenase; D000429:Alcohol Oxidoreductases / Q000235:genetics* / Q000502:physiology; D000438:Alcohols / Q000494:pharmacology; D000818:Animals; D030801:Animals, Genetically Modified; D004175:Diptera / Q000187:drug effects / Q000235:genetics; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000201:enzymology* / Q000235:genetics; D005260:Female; D015870:Gene Expression; D018014:Gene Transfer Techniques; D008297:Male
D000438:Alcohols; D029721:Drosophila Proteins; D000429:Alcohol Oxidoreductases; C073796:ADH protein, Drosophila; D000426:Alcohol Dehydrogenase