Cancer chemopreventive agents, 4-phenylcoumarins from Calophyllum inophyllum.
In a search for anti-tumor-promoting agents, we carried out a primary screening of ten 4-phenylcoumarins isolated from Calophyllum inophyllum L. (Guttiferae), by examining their possible inhibitory effects on Epstein--Barr virus early antigen (EBV-EA) activation induced by 12-O-tetradecanoylphorbol-13-acetate in Raji cells. All of the compounds tested in this study showed inhibitory activity against EBV, without showing any cytotoxicity. Calocoumarin-A (5) showed more potent activity than any of the other compounds tested. Furthermore, calocoumarin-A (5) exhibited a marked inhibitory effect on mouse skin tumor promotion in an in vivo two-stage carcinogenesis test. The results of the present investigation indicate that some of these 4-phenylcoumarins might be valuable as potential cancer chemopreventive agents (anti-tumor-promoters).
Itoigawa
M
M
Faculty of Pharmacy, Meijo University, Tempaku, Nagoya 468-8503, Japan. itoigawa@tokaigakuen-u.ac.jp
Ito
C
C
Tan
H T
HT
Kuchide
M
M
Tokuda
H
H
Nishino
H
H
Furukawa
H
H
D000818:Animals; D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D000956:Antigens, Viral / Q000378:metabolism; D002273:Carcinogens; D003374:Coumarins / Q000494:pharmacology*; D005260:Female; D004854:Herpesvirus 4, Human / Q000254:growth & development / Q000276:immunology; D006801:Humans; D051379:Mice; D008813:Mice, Inbred ICR; D010212:Papilloma / Q000139:chemically induced / Q000517:prevention & control; D010936:Plant Extracts / Q000494:pharmacology; D010946:Plants, Medicinal / Q000737:chemistry; D012878:Skin Neoplasms / Q000139:chemically induced / Q000517:prevention & control; D013755:Tetradecanoylphorbol Acetate; D014197:Trees / Q000737:chemistry*; D014775:Virus Activation / Q000187:drug effects
D016588:Anticarcinogenic Agents; D000956:Antigens, Viral; D002273:Carcinogens; D003374:Coumarins; C033305:Epstein-Barr virus early antigen; D010936:Plant Extracts; C094411:4-phenylcoumarin; D013755:Tetradecanoylphorbol Acetate