title: Extended exposure of adult and fetal mice to 50 Hz magnetic field does not increase the incidence of micronuclei in erythrocytes.
abstract: The flow cytometer-based micronucleus assay was used to study the effects on chromosomes in erythroid cells of CBA/Ca mice after extended exposure to 50 Hz magnetic field (MF), 14 microT, peak-to-peak (p-p). The study included two different experiments: (a) mice exposed in utero during 18 days of their prenatal stage, and (b) adult mice exposed for 18 days. In experiment (a) 35 days after exposure was terminated, peripheral blood was drawn from the mice exposed in utero to determine whether the exposure had a genotoxic effect on the pluripotent erythroid stem cells. About 200000 polychromatic erythrocytes (PCE) and 200000 normochromatic erythrocytes (NCE) were analysed from each of 20 exposed mice. The EMF exposure did not significantly change the frequency of micronucleated PCE or NCE in comparison with 20 sham-irradiated mice. There was no difference in the proportion of PCE between exposed and unexposed animals. Similarly, in experiment (b) no differences were seen between EMF exposed and unexposed adult mice when samples of peripheral blood were taken at the end of exposure and analyzed for micronuclei in PCE and NCE. The proportion of PCE was the same in both groups. The results indicate that exposure to EMF does not induce direct or indirect effects on chromosomes in erythroid cells expressed as increased levels of micronucleated erythrocytes of mice. No indications of delayed genetic effects were found.
authors: Department of Environmental Toxicology, Uppsala University, Uppsala, Sweden.
authors: L
authors: L
authors: Abramsson-Zetterberg
authors: J
authors: J
authors: Grawé
mesh_terms: D000818:Animals; D004912:Erythrocytes / Q000528:radiation effects / Q000648:ultrastructure*; D005260:Female; D005312:Fetal Blood / Q000166:cytology / Q000528:radiation effects; D005720:Gamma Rays / Q000009:adverse effects; D008280:Magnetics / Q000009:adverse effects*; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D048629:Micronuclei, Chromosome-Defective / Q000528:radiation effects / Q000648:ultrastructure*; D015162:Micronucleus Tests; D011247:Pregnancy