Genetic relatedness of VP1 genes of Australian and Taiwanese rotavirus isolates.
Gene 1 (which encodes the viral RNA-dependent RNA polymerase, VP1) of an atypical human reassortant rotavirus strain, E210 (serotype G2P1B), is unrelated to genes 1 of standard human rotaviruses. To ascertain the origin of this gene, we determined a partial sequence and found that it exhibited greatest identity to gene 1 of a Taiwanese isolate, TE83, which is representative of G2 strains that caused an epidemic of gastroenteritis in 1993. Limited sequence identity to genes 1 of standard human and animal viruses was observed. This was confirmed by phylogenetic analysis. However, hybridization analysis using an E210 gene 1-specific probe indicated that a related gene was found among other Australian G2 isolates and in a Japanese strain isolated in the 1970s.
Masendycz
P J
PJ
Department of Gastroenterology and Clinical Nutrition, Murdoch Children's, Research Institute, Parkville, Vic, Australia.
Palombo
E A
EA
D001315:Australia; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D005814:Genes, Viral; D006801:Humans; D010802:Phylogeny; D012330:RNA, Double-Stranded / Q000235:genetics / Q000302:isolation & purification; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012401:Rotavirus / Q000145:classification* / Q000201:enzymology / Q000235:genetics*; D013624:Taiwan; D014758:Viral Core Proteins / Q000235:genetics*; D015678:Viral Structural Proteins / Q000235:genetics
D012330:RNA, Double-Stranded; D012367:RNA, Viral; C069187:VP1 protein, Rotavirus; D014758:Viral Core Proteins; D015678:Viral Structural Proteins; D012321:DNA-Directed RNA Polymerases