X-ray microanalysis of airway surface liquid collected in cystic fibrosis mice.
The airway surface liquid (ASL) that lines the airway surface epithelium plays a major role in airway antibacterial defense and mucociliary transport efficiency, two key factors in cystic fibrosis (CF) disease. A major difficulty is to collect ASL in native conditions without stimulation or alteration of the underlying airway epithelium. Using a cryoprobe specifically adapted to collect native ASL from the tracheal mouse surface, we analyzed by X-ray microanalysis the complete ASL and plasma ion content in Cftr(tm1Hgu)/Cftr(tm1Hgu) mice compared with that in control littermates. ASL ion content from eight Cftr(tm1Hgu)/Cftr(tm1Hgu) mice and eight control littermates did not appear significantly different. The mean (+/-SE) concentrations were 2,352 +/- 367 and 2,058 +/- 401 mmol/kg dry weight for Na, 1,659 +/- 272 and 1,448 +/- 281 mmol/kg dry weight for Cl, 357 +/- 57 and 337 +/- 38 mmol/kg dry weight for S, 1,066 +/- 220 and 787 +/- 182 mmol/kg dry weight for K, 400 +/- 82 and 301 +/- 58 mmol/kg dry weight for Ca, 105 +/- 31 and 105 +/- 20 mmol/kg dry weight for Mg, 33 +/- 15 and 29 +/- 9 mmol/kg dry weight for P in non-CF and CF mice, respectively. This cryotechnique appears to be a promising technique for analyzing the complete elemental composition of native ASL in CF and non-CF tissues.
Zahm
J M
JM
Institut National de la Santé et de la Recherche Médicale Unité 514, Reims, France. epuche@worldnet.fr
Baconnais
S
S
Davidson
D J
DJ
Webb
S
S
Dorin
J
J
Bonnet
N
N
Balossier
G
G
Puchelle
E
E
D000818:Animals; D001826:Body Fluids / Q000378:metabolism*; D003550:Cystic Fibrosis / Q000378:metabolism*; D004577:Electron Probe Microanalysis; D007477:Ions; D051379:Mice; D018181:Mice, Inbred CFTR; D012016:Reference Values; D014132:Trachea / Q000378:metabolism*
D007477:Ions