title: High-density molecular map of chromosome region harboring stripe-rust resistance genes YrH52 and Yr15 derived from wild emmer wheat, Triticum dicoccoides.
abstract: Two stripe-rust resistance genes, YrH52 and Yr15, derived from the Israeli wild emmer wheat, Triticum dicoccoides, have been located on chromosome 1B. The main objectives of the present study were to increase marker density in the vicinity of YrH52 gene by means of AFLP, RAPD and microsatellite markers, to improve the map of another T dicoccoides-derived stripe-rust resistance gene Yr15 using microsatellite markers, and to preliminarily discriminate these two genes. Additional 26 marker loci comprising 20 AFLPs, three RAPDs, and three microsatellites were found to be linked to YrH52 gene. An updated genetic map consisting of 45 marker loci, in the region of YrH52 gene, was constructed with a total map length of 107.7 cm. The mean interval length was 0.96 cm in the region Xgwm359b-P55M53b carrying YrH52 gene. YrH52 was bracketed by Xgwm413 (Nor1 and UBC212a) and Xgwm273a (Xgwm273d) with map distance of 1.3 and 2.7 cm from either side, respectively. Eight additional microsatellite markers were found to be linked with Yr15, and the linkage map of Yr15 gene was thus obviously improved. In the YrH52-mapping population, no crossover was detected in the interval UBC212a (Xgwm413)-Yr15-Nor1, and YrH52 was located distally outside this interval. It may suggest that YrH52 is different from Yr15 even though both of them are derived from T. dicoccoides and are mapped on chromosome 1BS. The large number of molecular makers revealed in the present study would be helpful for the marker-assisted introgression of the T. dicoccoides-derived YrH52 and Yr15 stripe-rust resistance genes into elite cultivars of wheat, and the high-density map would accelerate the map-based cloning of the two genes.
authors: Institute of Evolution, University of Haifa, Mount Carmel, Israel.
authors: J H
authors: JH
authors: Peng
authors: T
authors: T
authors: Fahima
authors: Röder
authors: Q Y
authors: QY
authors: Huang
authors: A
authors: A
authors: Dahan
authors: Y C
authors: YC
authors: Li
authors: A
authors: A
authors: Grama
authors: E
authors: E
authors: Nevo
mesh_terms: D001487:Basidiomycota; D002874:Chromosome Mapping; D017343:Genes, Plant; D005819:Genetic Markers; D018895:Microsatellite Repeats; D010935:Plant Diseases / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique; D014908:Triticum / Q000235:genetics*
chemical_list: D005819:Genetic Markers