The relationship between the magnitude of the specific antibody response to experimental salmonella enteritidis infection in laying hens and their production of contaminated eggs.
Detecting infected laying flocks is a vital part of many efforts to control egg-associated transmission of Salmonella enteritidis to humans. The relationship between the development of a specific antibody response in infected hens and the deposition of S. enteritidis in eggs is important for establishing the epidemiologic relevance of serologic testing methods. In two trials, laying hens were infected with large oral doses of phage types 13a and 14b isolates of S. enteritidis. Approximately 38% of all infected hens produced at least one contaminated egg, at an overall incidence of 5.2%, between 3 and 23 days postinoculation. As determined by enzyme-linked immunosorbent assay with an S. enteritidis flagellar antigen, 91.7% of inoculated hens produced specific serum antibodies. Although hens with very high antibody titers were associated with a significantly elevated frequency of egg contamination, a consistently direct relationship was not evident between the magnitude of the antibody responses of individual hens and the frequency at which they laid contaminated eggs. Accordingly, although serologic tests can be valuable screening tools for preliminary detection of S. enteritidis infections in poultry, the magnitude of the antibody responses detected in individual hens may not predict the overall risk of egg contamination associated with particular laying flocks.
Gast
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RK
United States Department of Agriculture, Agricultural Research Service, Southeast Poultry Research Laboratory, Athens, GA 30605, USA.
Holt
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D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D002645:Chickens; D004531:Eggs / Q000382:microbiology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005243:Feces / Q000382:microbiology; D005260:Female; D005516:Food Microbiology; D015994:Incidence; D018445:Infectious Disease Transmission, Vertical / Q000662:veterinary; D010058:Oviposition; D011201:Poultry Diseases / Q000276:immunology* / Q000382:microbiology / Q000635:transmission; D011237:Predictive Value of Tests; D011336:Probability; D012481:Salmonella Infections, Animal / Q000175:diagnosis / Q000276:immunology* / Q000635:transmission; D012477:Salmonella enteritidis / Q000276:immunology* / Q000302:isolation & purification; D013997:Time Factors
D000907:Antibodies, Bacterial