MIB-1 immunoreactivity correlates with biologic behaviour in canine cutaneous melanoma.
The growth fraction of 68 canine cutaneous melanomas was determined by immunostaining with MIB-1, a monoclonal antibody to a Ki-67 epitope that recognizes all proliferating cells. Fifty tumours were classified histologically as benign and 18 as malignant. The Ki-67 proliferative index (percentage of positive cells over 500 neoplastic cells) was low (< 15%) in 55 cases and high (> or = 15%) in 13 cases. High Ki-67 proliferative index and histological malignancy were both associated with significantly poorer 2-year survival (P < 0.0001). However, the predictive value of the Ki-67 proliferative index (97%) was higher than the predictive value of classical histology (91%). The evaluation of the growth fraction by the Ki-67 proliferative index is highly predictive of the biological behaviour of canine cutaneous melanoma.
Laprie
C
C
Unité d'Anatomie pathologique associée INRA sécurité alimentaire, Ecole Nationale Vétérinaire de Toulouse, 23 Chemin des Capelles, 31076 Toulouse, France.
Abadie
J
J
Amardeilh
M F
MF
Net
J L
JL
Lagadic
M
M
Delverdier
M
M
D000818:Animals; D034961:Antigens, Nuclear; D014408:Biomarkers, Tumor / Q000276:immunology*; D004283:Dog Diseases / Q000175:diagnosis* / Q000276:immunology / Q000401:mortality*; D004285:Dogs; D005260:Female; D007150:Immunohistochemistry / Q000662:veterinary; D019394:Ki-67 Antigen; D008297:Male; D008545:Melanoma / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*; D009687:Nuclear Proteins / Q000276:immunology*; D011379:Prognosis; D012189:Retrospective Studies; D012878:Skin Neoplasms / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*; D016019:Survival Analysis
D034961:Antigens, Nuclear; D014408:Biomarkers, Tumor; D019394:Ki-67 Antigen; D009687:Nuclear Proteins