title: Proteomics--post-genomic cartography to understand gene function.
abstract: The completion of the genomic sequences of numerous organisms from human and mouse to Caenorhabditis elegans and many microorganisms, and the definition of their genes provides a database to interpret cellular protein-expression patterns and relate them to protein function. Proteomics technologies that are dependent on mass spectrometry and involve two-dimensional gel electrophoresis are providing the main window into the world of differential protein-expression analysis. In this article, the limitations and expectations of this research field are examined and the future of the analytical needs of proteomics is explored.
authors: Ludwig Institute for Cancer Research and Dept of Biochemistry and Molecular Biology, Royal Free and University College London Medical School, 91 Riding House Street, London, UK W1W 7BS.
authors: S
authors: S
authors: Naaby-Hansen
authors: M D
authors: MD
authors: Waterfield
authors: R
authors: R
authors: Cramer
mesh_terms: D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods / Q000639:trends; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods / Q000639:trends; D010449:Peptide Mapping / Q000379:methods* / Q000639:trends; D020543:Proteome / Q000032:analysis*; D016324:Sequence Tagged Sites; D015398:Signal Transduction / Q000502:physiology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods* / Q000639:trends
chemical_list: D020543:Proteome