Characterization of cDNA of the liverwort phytochrome gene, and phytochrome involvement in the light-dependent and light-independent protochlorophyllide oxidoreductase gene expression in Marchantia paleacea var. diptera.
The cDNA of the phytochrome gene in the liverwort Marchantia paleacea var. diptera (MpdPHY1) was isolated. MpdPHY1 encoded a conventional phytochrome apoprotein. The MpdPHY1 transcript was accumulated in the dark and suppressed in the light. The degradation of the MpdPHY1 transcript by red light irradiation had red/far-red reversibility, suggesting that the liverwort phytochrome gene expression was regulated by a phytochrome. Northern blot analysis of the transcripts in cells irradiated by red/far-red light revealed that the liverwort phytochrome was involved in the expressions of chlB, chlL, chlN, or por, which encode subunits of light-independent and light-dependent protochlorophyllide oxidoreductase, respectively.
Suzuki
T
T
Department of Biological Science, Graduate School of Science, Hiroshima University, Higashi-Hiroshima, 739-8526 Japan.
Takio
S
S
Yamamoto
I
I
Satoh
T
T
D000595:Amino Acid Sequence; D001059:Apoproteins / Q000235:genetics* / Q000378:metabolism; D015139:Blotting, Southern / Q000379:methods; D003001:Cloning, Molecular; D018076:DNA, Complementary; D003624:Darkness; D015971:Gene Expression Regulation, Enzymologic; D017343:Genes, Plant; D008027:Light; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000235:genetics*; D044925:Oxidoreductases Acting on CH-CH Group Donors; D010834:Phytochrome / Q000235:genetics* / Q000378:metabolism; D010940:Plant Proteins; D010944:Plants / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D017422:Sequence Analysis, DNA
D001059:Apoproteins; D018076:DNA, Complementary; C443669:PHY1 protein, Marchantia paleacea; D010940:Plant Proteins; D012333:RNA, Messenger; D010834:Phytochrome; D010088:Oxidoreductases; D044925:Oxidoreductases Acting on CH-CH Group Donors; C023886:protochlorophyllide reductase