title: Reconstitution of Sec-dependent membrane protein insertion: nascent FtsQ interacts with YidC in a SecYEG-dependent manner.
abstract: The inner membrane protein YidC is associated with the preprotein translocase of Escherichia coli and contacts transmembrane segments of nascent inner membrane proteins during membrane insertion. YidC was purified to homogeneity and co-reconstituted with the SecYEG complex. YidC had no effect on the SecA/SecYEG-mediated translocation of the secretory protein proOmpA; however, using a crosslinking approach, the transmembrane segment of nascent FtsQ was found to gain access to YidC via SecY. These data indicate the functional reconstitution of the initial stages of YidC-dependent membrane protein insertion via the SecYEG complex.
authors: Department of Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kerklaan 30, 9751 NN Haren The Netherlands.
authors: M
authors: M
authors: van der Laan
authors: E N
authors: EN
authors: Houben
authors: N
authors: N
authors: Nouwen
authors: J
authors: J
authors: Luirink
authors: A J
authors: AJ
authors: Driessen
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism*; D002462:Cell Membrane / Q000201:enzymology / Q000378:metabolism; D003062:Codon; D003432:Cross-Linking Reagents / Q000494:pharmacology; D019281:Dimerization; D004926:Escherichia coli / Q000201:enzymology / Q000378:metabolism; D029968:Escherichia coli Proteins; D008565:Membrane Proteins / Q000378:metabolism*; D026901:Membrane Transport Proteins; D010957:Plasmids / Q000378:metabolism; D011485:Protein Binding; D014176:Protein Biosynthesis; D011510:Proteolipids / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D000069816:SEC Translocation Channels; D000081416:SecA Proteins; D014158:Transcription, Genetic; D014178:Translocation, Genetic
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D003062:Codon; D003432:Cross-Linking Reagents; D029968:Escherichia coli Proteins; C068023:FtsQ protein, E coli; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D011510:Proteolipids; D012333:RNA, Messenger; D000069816:SEC Translocation Channels; C065493:SecE protein, E coli; C088616:SecG protein, E coli; C057841:SecY protein, E coli; C412443:YIDC protein, E coli; C020485:proteoliposomes; D000251:Adenosine Triphosphatases; D000081416:SecA Proteins