Establishment of an animal embryo culture system containing various embryotropins and its efficacy for culturing ICR mouse one-cell embryos derived in vivo or in vitro.
OBJECTIVE
To develop an effective ICR mouse embryo culture medium.


METHODS
In vitro model study.


METHODS
University-affiliated hospital.


METHODS
Four-week-old, superovulated mice.


METHODS
In vivo- or in vitro-derived one-cell embryos were cultured in preimplantation-1 medium (P-1).


METHODS
Preimplantation development.


RESULTS
In vivo-derived embryos were cultured in BSA-containing P-1, to which one of the following substances was added: [1] no addition, [2] amino acids (aa), [3] aa+hemoglobin (hb), [4] aa+hb+cysteine (cys), [5] aa+hb and glucose (glu) added at the four-cell, or [6] aa+hb and glu+cys added at the four-cell stage. More (P<0.05) blastocysts developed after aa or aa+hb addition than after no addition, and glu addition to such medium further stimulated the formation (54%). In P-1 with aa+glu, the addition of 1 microg/mL hb was optimal. Additional improvement of blastocyst formation (78%) was achieved by ethylenediaminetetraacetic acid (EDTA), supplementation and bovine serum albumin replacement with polyvinyl alcohol (PVA) did not inhibit the development. P-1 supplemented with aa, hb, glu, EDTA, and PVA also supported the development of in vitro-derived embryos (70%).


CONCLUSIONS
A modified P-1 medium was developed, and it supported the development of both in vivo- and in vitro-derived ICR mouse embryos.
Park
S E
SE
College of Medicine, Pochon CHA University and Infertility Medical Center of CHA General Hospital, Seoul, South Korea.
Chung
H M
HM
Cha
K Y
KY
Hwang
W S
WS
Lee
E S
ES
Lim
J M
JM
D000596:Amino Acids / Q000008:administration & dosage; D000818:Animals; D001755:Blastocyst / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D003470:Culture Media / Q000737:chemistry* / Q000494:pharmacology*; D046508:Culture Techniques; D004338:Drug Combinations; D004492:Edetic Acid / Q000008:administration & dosage; D004622:Embryo, Mammalian / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D047108:Embryonic Development; D005314:Embryonic and Fetal Development; D005260:Female; D005307:Fertilization in Vitro; D005947:Glucose / Q000008:administration & dosage; D006133:Growth Substances / Q000378:metabolism*; D006454:Hemoglobins / Q000008:administration & dosage; D051379:Mice; D008813:Mice, Inbred ICR; D011142:Polyvinyl Alcohol / Q000008:administration & dosage; D011247:Pregnancy; D012710:Serum Albumin, Bovine / Q000008:administration & dosage
D000596:Amino Acids; D003470:Culture Media; D004338:Drug Combinations; D006133:Growth Substances; D006454:Hemoglobins; D012710:Serum Albumin, Bovine; D011142:Polyvinyl Alcohol; D004492:Edetic Acid; D005947:Glucose