title: Latanoprost is a vasoconstrictor in isolated porcine ciliary arteries.
abstract: OBJECTIVE
  The purpose of this study was to assess the vasoconstrictor effect of the prostaglandin F2 alpha analog latanoprost in isolated porcine ciliary arteries.
  
  
  METHODS
  With a myograph system to measure isometric forces, porcine ciliary arteries were exposed, in a cumulative manner, to increasing concentrations of latanoprost (0.1 nM-0.1 mM). Experiments were conducted in quiescent vessels as well as in vessels precontracted with potassium chloride (KCl; 40 mM) or endothelin-1 (10 nM). Contractions were expressed in percent of 100 mM KCl-induced contraction.
  
  
  RESULTS
  In a concentration dependent manner latanoprost induced significant (p < 0.001) contractions in quiescent vessels (maximum: 68 +/- 3%, n = 5; PD50: 5.7 +/- 0.1). In KCl or in endothelin-1 precontracted vessels latanoprost did not evoke any relaxation but in contrast significant (p < 0.001) contractions (maximum: 33 +/- 4%, n = 5 and 26 +/- 6%, n = 8, respectively).
  
  
  CONCLUSIONS
  Latanoprost has a vasoconstrictor effect in isolated porcine ciliary arteries. No relaxation could be observed. The clinical relevance of this finding requires further evaluation.
authors: Laboratory of Ocular Pharmacology and Physiology, University Eye Clinic Basel, Mittlere Strasse 91, PO Box, CH-4012 Basel, Switzerland.
authors: G
authors: G
authors: Brogiolo
authors: J
authors: J
authors: Flammer
authors: I O
authors: IO
authors: Haefliger
mesh_terms: D000818:Animals; D019842:Ciliary Arteries / Q000187:drug effects*; D046508:Culture Techniques; D015237:Dinoprost / Q000031:analogs & derivatives*; D004305:Dose-Response Relationship, Drug; D007429:Intraocular Pressure / Q000187:drug effects; D000077338:Latanoprost; D011461:Prostaglandins F, Synthetic / Q000494:pharmacology*; D013552:Swine; D014662:Vasoconstrictor Agents / Q000494:pharmacology*
chemical_list: D011461:Prostaglandins F, Synthetic; D014662:Vasoconstrictor Agents; D000077338:Latanoprost; D015237:Dinoprost