[Complete genome sequence analysis of the Hantavirus Z10 strain].
OBJECTIVE
Study on the complete genome sequence of Hantavirus Z10 strain which has been applied for inactivated vaccine production in China, to assess its molecular characteristics and the diversity with other hantaviruses.


METHODS
The total RNA were prepared from Z10 virus infected cells and the RT-PCR products was cloned into T vector, sequenced and analyzed by using DNASTAR software.


RESULTS
The Z10 complete genome, L segment is 6,553, M segment is 3,615, S segment is 1,701 nucleotides in length, with a single open reading frame encoding 2,151, 1,135, 429 amino acids respectively. Sequence homology comparison showed that the 3 segment nucleotide of Z10 strain were close to HTN type virus, but only 83.6-87.4% homology with other HTN viruses at the nucleotide level. The phylogenetic analysis was made on their nucleotide and amino acid sequences.


CONCLUSIONS
The results firstly demonstrates that Z10 strain is a new subtype of the Hantaan(HTN) type.
Yao
Z
Z
National Institute for the Control of Pharmaceutical and Biological Pruducts, Beijing 100050, China.
Yu
Y
Y
Dong
G
G
D000595:Amino Acid Sequence; D001483:Base Sequence; D016679:Genome, Viral; D006476:Orthohantavirus / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D010802:Phylogeny; D017385:Sequence Homology