HMGN4, a newly discovered nucleosome-binding protein encoded by an intronless gene.
We describe a newly discovered nuclear protein, HMGN4, that is closely related to the canonical HMGN2 nucleosome-binding protein. The protein is encoded by an intronless gene, which, in humans, is located in the hereditary hemochromatosis [correction of hemachromatosis] region at position 6p21.3. A single approximately 2-kb HMGN4 mRNA was found to be expressed, in variable amounts, in all human tissues tested; however, the HMGN4 transcript was significantly less abundant than that of HMGN2. The HMGN4 protein could be detected in HeLa cells by Western analysis with an antibody elicited against a unique region of the protein. Transfection of HeLa cells with a plasmid expressing HMGN4-GFP indicated that the protein localizes to the nucleus. Our results expand the multiplicity of the HMGN protein family and increase the known cellular repertoire of nucleosome-binding proteins.
Birger
Y
Y
Protein Section, Laboratory of Metabolism, Division of Basic Sciences, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA. birgery@pop.nci.nih.gov
Ito
Y
Y
West
K L
KL
Landsman
D
D
Bustin
M
M
D000818:Animals; D015152:Blotting, Northern; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D017930:Genes, Reporter; D024001:HMGN Proteins; D006367:HeLa Cells; D006432:Hemochromatosis / Q000235:genetics; D006801:Humans; D015151:Immunoblotting; D007438:Introns / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D009707:Nucleosomes / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D014018:Tissue Distribution; D014162:Transfection
D002352:Carrier Proteins; D024001:HMGN Proteins; C028567:HMGN4 protein, human; D009707:Nucleosomes; D011993:Recombinant Fusion Proteins