title: Decreased T lymphocyte infiltration in bronchial biopsies of subjects with severe chronic obstructive pulmonary disease.
abstract: BACKGROUND
  Studies on the inflammatory process in the large airways of patients with mild/moderate COPD have shown a prevalent T lymphocyte and macrophage infiltration of the bronchial mucosa. However, bronchial inflammation in more severe disease has not been extensively studied.
  
  
  OBJECTIVE
  The aim of the present study was to characterize the lymphocyte infiltration in the bronchial mucosa of subjects with severe, compared to mild, COPD, and to examine the relationship between airflow limitation and T lymphocyte numbers in the bronchial mucosa.
  
  
  METHODS
  We examined bronchial biopsies obtained from nine smokers with severe airflow limitation, nine smokers with mild/moderate airflow limitation and 14 smokers with normal lung function. Immunohistochemical methods on cryostat sections were used to assess the number of CD3+, CD4+, CD8+ cells and the number of CD3+ cells coexpressing the chemokine receptor CCR5 (CCR5+CD3+) in the subepithelium.
  
  
  RESULTS
  Subjects with severe COPD had lower numbers of CD3+, CD8+ and CCR5+CD3+ cells than mild/moderate COPD (P < 0.012, P < 0.02 and P < 0.02, respectively) and control smokers (P < 0.015, P < 0.005 and P < 0.015, respectively). In subjects with airflow limitation the number of CD3+ and CD8+ cells was inversely correlated with the degree of airway obstruction (r = 0.59, P < 0.015 and r = 0.52, P < 0.032, respectively).
  
  
  CONCLUSIONS
  Bronchial inflammation in severe COPD is characterized by lower numbers of CD3+ and CD8+ cells and decreased numbers of CD3+ cells coexpressing the chemokine receptor CCR5. T lymphocyte infiltration is inversely correlated with the degree of airflow limitation.
authors: Salvatore Maugeri Foundation, IRCCS, Medical Center of Rehabilitation, Division of Pulmonary Disease, Veruno, Italy. adistefano@fsm.it
authors: A
authors: A
authors: Di Stefano
authors: A
authors: A
authors: Capelli
authors: M
authors: M
authors: Lusuardi
authors: G
authors: G
authors: Caramori
authors: P
authors: P
authors: Balbo
authors: F
authors: F
authors: Ioli
authors: S
authors: S
authors: Sacco
authors: I
authors: I
authors: Gnemmi
authors: P
authors: P
authors: Brun
authors: I M
authors: IM
authors: Adcock
authors: B
authors: B
authors: Balbi
authors: P J
authors: PJ
authors: Barnes
authors: K F
authors: KF
authors: Chung
authors: C F
authors: CF
authors: Donner
mesh_terms: D000368:Aged; D001706:Biopsy; D015153:Blotting, Western; D001980:Bronchi / Q000378:metabolism* / Q000473:pathology*; D017252:CD3 Complex / Q000032:analysis; D016827:CD8 Antigens / Q000032:analysis; D002465:Cell Movement; D005260:Female; D005541:Forced Expiratory Volume / Q000502:physiology; D006801:Humans; D007150:Immunohistochemistry; D008173:Lung Diseases, Obstructive / Q000453:epidemiology / Q000378:metabolism* / Q000473:pathology*; D018655:Lymphocyte Count; D008297:Male; D008875:Middle Aged; D019713:Receptors, CCR5 / Q000032:analysis; D012720:Severity of Illness Index; D012907:Smoking / Q000378:metabolism / Q000473:pathology; D013223:Statistics as Topic; D013601:T-Lymphocytes / Q000166:cytology* / Q000276:immunology
chemical_list: D017252:CD3 Complex; D016827:CD8 Antigens; D019713:Receptors, CCR5