Characterization of the extracellular enzyme systems of Trichoderma viride AH124.
A mycoparasitic Trichoderma viride strain was investigated for the production of extracellular enzymes important in antagonism, by using natural and chromogenic substrates. Some of these enzymes, such as beta-1,3-glucanases, and low levels of proteases were produced constitutively. Under inductive conditions, the measurable activities of beta-1,3-glucanase, protease and aspecific chitinase increased, while for the proteases and beta-1,3-glucanases, the levels depended on both the nitrogen and the carbon source. Gel filtration chromatography revealed at least 4 beta-1,3-glucanases, 6 proteases, 2 beta-glucosidases and 1 beta-1,4-N-acetyl-glucosaminidase izoenzyme under inductive conditions.
Manczinger
L
L
Department of Microbiology, Faculty of Sciences, University of Szeged, Hungary. manczing@bio.u-szeged.hu
Antal
Z
Z
Schoop
A
A
Kredics
L
L
D003470:Culture Media; D010450:Endopeptidases / Q000302:isolation & purification; D004790:Enzyme Induction; D006026:Glycoside Hydrolases / Q000302:isolation & purification; D014242:Trichoderma / Q000201:enzymology*; D003313:Zea mays / Q000382:microbiology
D003470:Culture Media; D006026:Glycoside Hydrolases; D010450:Endopeptidases