BAP37 and Prohibitin are specifically recognized by an SV40 T antigen antibody.
We have identified two cellular proteins that are specifically immunoprecipitated by an anti-SV40 T antigen monoclonal antibody. This antibody, PAb419, recognizes an epitope contained within a region of T antigen which we have recently demonstrated is required for the initiation of immortalization by SV40 T antigen, but is not essential for maintenance of the immortal state. The two proteins were identified as BAP37 and Prohibitin. Recent results suggest Prohibitin may enhance the transcriptional inactivation of E2F by the retinoblastoma family of pocket proteins (pRb, p107, p130). BAP37 and Prohibitin are specifically recognized by PAb419 and PAb210, another anti-SV40 T antigen monoclonal antibody, which has an overlapping epitope, but not by other anti-SV40 T antigen monoclonal antibodies, demonstrating the specificity of the interaction.
Darmon
A J
AJ
Ludwig Institute for Cancer Research, University College School of Medicine, Courtauld Building, 91 Riding House Street, London, W1W 7BS, United Kingdom.
Jat
P S
PS
D016475:3T3 Cells; D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism*; D000918:Antibody Specificity; D000936:Antigen-Antibody Complex / Q000737:chemistry; D000952:Antigens, Polyomavirus Transforming / Q000276:immunology; D000957:Antigens, Viral, Tumor / Q000276:immunology*; D015153:Blotting, Western; D002461:Cell Line, Transformed / Q000737:chemistry; D000939:Epitopes / Q000276:immunology; D051379:Mice; D011233:Precipitin Tests; D000091402:Prohibitins; D011506:Proteins / Q000276:immunology*; D012097:Repressor Proteins; D020539:Sequence Analysis, Protein
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D000952:Antigens, Polyomavirus Transforming; D000957:Antigens, Viral, Tumor; D000939:Epitopes; C000717333:Phb2 protein, mouse; D000091402:Prohibitins; D011506:Proteins; D012097:Repressor Proteins