title: Dual control of hydrogen cyanide biosynthesis by the global activator GacA in Pseudomonas aeruginosa PAO1.
abstract: The global response regulator GacA of Pseudomonas aeruginosa PAO1 positively controls the production of the quorum sensing signal molecule N-butanoyl-homoserine-lactone (C4-HSL) and hence the synthesis of several C4-HSL-dependent virulence factors, including hydrogen cyanide (HCN). This study presents evidence that GacA positively influences the transcription of the rhlI gene, specifying C4-HSL synthase, explaining the quorum sensing-dependent transcriptional control of the HCN biosynthetic genes (hcnABC). In addition, GacA was found to modulate hcn gene expression positively at a post-transcriptional level involving the hcnA ribosome-binding site. Thus, the activating effect of GacA on cyanogenesis results from both transcriptional and post-transcriptional mechanisms.
authors: Laboratoire de Biologie Microbienne, Université de Lausanne, CH-1015 Lausanne, Switzerland.
authors: G
authors: G
authors: Pessi
authors: D
authors: D
authors: Haas
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives / Q000096:biosynthesis / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D006856:Hydrogen Cyanide / Q000378:metabolism*; D009097:Multienzyme Complexes / Q000096:biosynthesis / Q000235:genetics*; D010088:Oxidoreductases / Q000096:biosynthesis / Q000235:genetics*; D050891:Oxidoreductases Acting on CH-NH2 Group Donors; D011550:Pseudomonas aeruginosa / Q000201:enzymology / Q000235:genetics*; D012323:RNA Processing, Post-Transcriptional
chemical_list: D001426:Bacterial Proteins; C091100:GacA protein, Bacteria; D009097:Multienzyme Complexes; C092312:N-butyrylhomoserine lactone; C119941:RhiI protein, Rhizobium leguminosarum; D006856:Hydrogen Cyanide; D010088:Oxidoreductases; D050891:Oxidoreductases Acting on CH-NH2 Group Donors; C112800:glycine dehydrogenase (cyanide-forming); D015107:4-Butyrolactone