[Determination of the minimum size of Gallus gallus domesticus chicken microchromosome by a pulse electrophoresis method].
The karyotype of the chicken Gallus gallus domesticus was studied by means of pulsed-field electrophoresis. An electrokaryogram was obtained for the microchromosomal (MI) portion of the chicken genome. Chicken MIs were separated into two fractions. A fraction with a higher mobility included MIs sized 3.4-4.8 Mb; the lower size limit of a less mobile fraction corresponded to MIs of approximately 5 Mb. The smallest MI in the chicken karyotype was estimated at 3.4 +/- 0.25 Mb.
Pichugin
A M
AM
Institute of Biology, St. Petersburg State University, St. Petersburg, 198904 Russia.
Galkina
S A
SA
Potekhin
A A
AA
Punina
E O
EO
Rautian
M S
MS
Rodionov
A V
AV
D000818:Animals; D002645:Chickens / Q000235:genetics*; D002875:Chromosomes; D016521:Electrophoresis, Gel, Pulsed-Field; D007621:Karyotyping; D012441:Saccharomyces cerevisiae / Q000235:genetics; D012568:Schizosaccharomyces / Q000235:genetics