Complexes formation between insulin receptor and extracellular signal-regulated kinases ERKs.
A property of signal transduction pathways that might explain their efficiency and specificity is the formation of signaling complexes. The recent demonstration that adaptor proteins can interact with many components of the extracellular signal-regulated kinases (ERKs) signaling cascade leads us to investigate whether such complexes may include the transmembrane receptor. The present work shows that in human hepatoma Hep3B cells, insulin receptor (IR) can be coimmunoprecipitated with other components of the ERKs cascade: insulin receptor substrate (IRS), Raf-1, and ERKs. Furthermore, these complexes formed near the cytoplasmic membrane even prior to insulin stimulation.
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Institut de Génétique Humaine, Centre National de la Recherche Scientifique, 141 rue de la Cardonille, Montpellier Cedex 5, 34396, France
Mettling
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Chou
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D015153:Blotting, Western; D006528:Carcinoma, Hepatocellular / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D046911:Macromolecular Substances; D019950:Mitogen-Activated Protein Kinase 1 / Q000032:analysis / Q000378:metabolism; D048052:Mitogen-Activated Protein Kinase 3; D020928:Mitogen-Activated Protein Kinases / Q000032:analysis / Q000378:metabolism*; D010766:Phosphorylation; D011233:Precipitin Tests; D019908:Proto-Oncogene Proteins c-raf / Q000032:analysis / Q000378:metabolism; D011972:Receptor, Insulin / Q000032:analysis / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology; D014407:Tumor Cells, Cultured
D046911:Macromolecular Substances; D011972:Receptor, Insulin; D019908:Proto-Oncogene Proteins c-raf; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; D020928:Mitogen-Activated Protein Kinases