A simple method to obtain a covalent immobilized phospholipase A(2).
In the present work, we obtained an immobilized phospholipase A(2) system through covalent coupling by using an acrylic polymer Eupergit C as support. The immobilized enzyme from cobra venom (Naja naja naja) showed good retention activity and excellent stability. Both properties are of great importance for biomedical applications such as hypercholesterolemia treatments.
Madoery
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Facultad de Ciencias Agropecuarias, Universidad Nacional de Córdoba, 5000, Córdoba, Argentina. rmadoery@agro.uncor.edu
Fidelio
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D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism*; D007700:Kinetics; D010741:Phospholipases A / Q000378:metabolism*
D004800:Enzymes, Immobilized; D010741:Phospholipases A