Development of preimplantation mouse embryos after exposure to a 50 Hz magnetic field in vitro.
Effect of sinusoidal 50 Hz magnetic field (MF) on development of preimplantation CBA/S mouse embryos in vitro was studied. Superovulated and in vivo fertilized preimplantation embryos were collected at one cell stage and divided to control and MF-exposed groups. Sinusoidal 50 Hz MF with field strength of 10 A/m r.m.s., corresponding a flux density of 13 microT r.m.s., was used to expose the embryos in culture at 37 degrees C in a CO2-incubator. The developmental stage and abnormalities were recorded twice daily except once daily during weekends. The vitality and developmental stages of the embryos were similar in both groups although slightly more dead embryos were found during the 1st day in MF-exposed group (P<0.05) and the development of MF-exposed embryos was slightly impaired. In conclusion, the exposure to sinusoidal 50 Hz MF at field strength of 10 A/m did not significantly disturb the development of the mouse embryos in vitro up to the blastocyst stage.
Huuskonen
H
H
National Public Health Institute, Department of Environmental Health, Laboratory of Toxicology, P.O. Box 95, FIN-70701, Kuopio, Finland. hannele.huuskonen@ktl.fi
Juutilainen
J
J
Komulainen
H
H
D000818:Animals; D001755:Blastocyst / Q000502:physiology / Q000528:radiation effects*; D005314:Embryonic and Fetal Development / Q000528:radiation effects*; D005260:Female; D066298:In Vitro Techniques; D008280:Magnetics; D051379:Mice; D008808:Mice, Inbred CBA; D011247:Pregnancy