In vivo PC3 overexpression by retroviral vector affects cell differentiation of rat cortical precursors.
The PC3 gene is a marker of dividing neuroepithelial (NE) cells. We transduced single cortical precursors of the ventricular zone (VZ) with a PC3-carrying retroviral vector at E16 stage, and analysed the effects of transgene expression on their progeny in 3-week-old animals. Unlike control-transduced cells, all viable PC3-transduced cells remained close to the ventricle and displayed a round-shaped, undifferentiated morphology.
Malatesta
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P
Dipartimento di Fisiologia e Biochimica, Sezione di Biologia Cellulare e dello Sviluppo, Università di Pisa, via Carducci 13, 56010 Ghezzano, Pisa, Italy.
Sgadò
P
P
Caneparo
L
L
Barsacchi
G
G
Cremisi
F
F
D000818:Animals; D016282:Aspartic Acid Endopeptidases / Q000235:genetics*; D002454:Cell Differentiation / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D002540:Cerebral Cortex / Q000166:cytology* / Q000196:embryology*; D005260:Female; D018507:Gene Expression Regulation, Developmental; D015967:Gene Expression Regulation, Viral; D005822:Genetic Vectors; D009474:Neurons / Q000166:cytology / Q000502:physiology; D011247:Pregnancy; D043484:Proprotein Convertases; D051381:Rats; D017207:Rats, Sprague-Dawley; D012190:Retroviridae / Q000235:genetics; D013234:Stem Cells / Q000166:cytology / Q000502:physiology
D043484:Proprotein Convertases; D016282:Aspartic Acid Endopeptidases