title: Rat protein tyrosine phosphatase eta physically interacts with the PDZ domains of syntenin.
abstract: The tyrosine phosphatase r-PTPeta is able to suppress the malignant phenotype of rat thyroid tumorigenic cell lines. To identify r-PTPeta interacting proteins, a yeast two-hybrid screening was performed and an insert corresponding to the full-length syntenin cDNA was isolated. It encodes a protein containing two PDZ domains that mediates the binding of syntenin to proteins such as syndecan, proTGF-alpha, beta-ephrins and neurofascin. We show that r-PTPeta is able to interact with syntenin also in mammalian cells, and although syntenin is a tyrosine-phosphorylated protein it is not a substrate of r-PTPeta. The integrity of both PDZ domains of syntenin and the carboxy-terminal region of r-PTPeta are required for the interaction between syntenin and r-PTPeta.
authors: Dipartimento di Medicina Sperimentale i Clinica, Università degli Studi di Catanzaro Magna Graecia, 88100 Catanzaro, Italy.
authors: R
authors: R
authors: Iuliano
authors: F
authors: F
authors: Trapasso
authors: I
authors: I
authors: Samà
authors: I
authors: I
authors: Le Pera
authors: M L
authors: ML
authors: Martelli
authors: F
authors: F
authors: Lembo
authors: M
authors: M
authors: Santoro
authors: G
authors: G
authors: Viglietto
authors: L
authors: L
authors: Chiariotti
authors: A
authors: A
authors: Fusco
mesh_terms: D002352:Carrier Proteins / Q000737:chemistry / Q000378:metabolism*; D002478:Cells, Cultured; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D011233:Precipitin Tests; D017434:Protein Structure, Tertiary; D017027:Protein Tyrosine Phosphatases / Q000378:metabolism*; D054631:Receptor-Like Protein Tyrosine Phosphatases, Class 3; D053676:Syntenins; D020798:Two-Hybrid System Techniques
chemical_list: D002352:Carrier Proteins; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; C109621:SDCBP protein, human; D053676:Syntenins; C486288:PTPRJ protein, human; D017027:Protein Tyrosine Phosphatases; D054631:Receptor-Like Protein Tyrosine Phosphatases, Class 3