Molecular cloning and characterization of a novel peptidylprolyl isomerase (cyclophilin)-like gene (PPIL3) from human fetal brain.
During the large-scale sequencing analysis of a human fetal brain cDNA library, we isolated two cDNA clones encoding two novel proteins, which show 52% and 72% identity to the cyclophilin isoform 10 of C. elegans, respectively. Sequence analysis revealed these two cDNA clones are two different splicing variants of a novel cyclophilin-like gene (PPIL3). The PPIL3 gene was identified on a completely sequenced BAC (GenBank accession AC005037) from chromosome 2q33 between STS markers stSG2762 (proximal) and SHGC-3074 (distal), oriented toward the telomere. The PPIL3 gene consisted of eight exons spanning more than 18 kb of genomic DNA. RT-PCR analysis indicated that PPIL3 was ubiquitously expressed in adult human tissues.
Zhou
Z
Z
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai, People's Republic of China.
Ying
K
K
Dai
J
J
Tang
R
R
Wang
W
W
Huang
Y
Y
Zhao
W
W
Xie
Y
Y
Mao
Y
Y
D020121:5' Untranslated Regions / Q000235:genetics; D017398:Alternative Splicing / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D001921:Brain / Q000196:embryology / Q000201:enzymology / Q000378:metabolism; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics; D002889:Chromosomes, Human, Pair 2 / Q000235:genetics*; D003001:Cloning, Molecular; D019295:Computational Biology; D021983:Cyclophilins / Q000737:chemistry / Q000235:genetics*; D005091:Exons / Q000235:genetics*; D020869:Gene Expression Profiling; D006801:Humans; D007438:Introns / Q000235:genetics*; D008969:Molecular Sequence Data; D020161:Physical Chromosome Mapping; D022821:RNA Splice Sites / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D016415:Sequence Alignment; D017421:Sequence Analysis; D016324:Sequence Tagged Sites
D020121:5' Untranslated Regions; D022821:RNA Splice Sites; D012333:RNA, Messenger; D021983:Cyclophilins; C431697:Ppil3 protein, rat