UVB-induced GM-CSF production is suppressed by dexamethasone in HaCaT cells.
BACKGROUND
Epidermal keratinocytes are important sources of a wide variety of cytokines that include the Granulocyte-macrophage Colony Stimulating Factor (GM-CSF). Glucocorticoids have been shown to inhibit the production of several cytokines. However, their effect on GM-CSF synthesis by keratinocytes is still unknown.


METHODS
The effects of glucocorticoid on GM-CSF production by keratinocytes were evaluated using ELISA and RT-PCR analysis.


RESULTS
GM-CSF secretion by HaCaT cells increased with increasing UVB exposure. Dexamethasone suppressed basal release of GM-CSE In addition, it strongly inhibited both the UVB-mediated augmentation of GM-CSF protein production and mRNA expression. Lincomycin enhanced slightly the inhibitory effect of dexamethasone on GM-CSF synthesis, while lincomycin itself had no effect on GM-CSF secretion.


CONCLUSIONS
Results showed that dexamethasone suppressed basal release and UVB-induced production of GM-CSF by keratinocytes.
Kim
D S
DS
Department of Dermatology, Seoul National University, College of Medicine, Korea.
Kim
H J
HJ
Choi
K H
KH
Chung
J H
JH
Kim
K H
KH
Par
K C
KC
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D017931:DNA Primers; D003907:Dexamethasone / Q000494:pharmacology*; D004797:Enzyme-Linked Immunosorbent Assay; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis*; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008034:Lincomycin / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014466:Ultraviolet Rays
D000900:Anti-Bacterial Agents; D000893:Anti-Inflammatory Agents; D017931:DNA Primers; D012333:RNA, Messenger; D003907:Dexamethasone; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D008034:Lincomycin