title: Evaluation of protoporphyrin IX production, phototoxicity and cell death pathway induced by hexylester of 5-aminolevulinic acid in Reh and HPB-ALL cells.
abstract: Production of protoporphyrin IX (PpIX) in human B-cell leukemia cell line (Reh) and T-cell lymphoma cell line (HPB-ALL) was studied by flow cytometry after incubation with 5-aminolevulinic acid (ALA) or its hexylester in vitro. Cell survival and cell death pathway were also investigated in these two cell lines by cell growth curves, flow cytometry, and electron microscopy after ALA hexylester-mediated photodynamic therapy. Both ALA and its hexylester could induce PpIX production in the two cell lines, but ALA hexylester was about 100 times more efficient than ALA. Reh cells appear to be more sensitive than HPB-ALL cells to ALA hexylester-mediated phototoxicity. Apoptosis was the major cell death pathway of Reh cells, while necrosis played a major role in the case of HPB-ALL cells.
authors: Lithuanian Oncology Center, Polocko, Vilnius 2007, Lithuania.
authors: Z
authors: Z
authors: Luksiene
authors: I
authors: I
authors: Eggen
authors: J
authors: J
authors: Moan
authors: J M
authors: JM
authors: Nesland
authors: Q
authors: Q
authors: Peng
mesh_terms: D000622:Aminolevulinic Acid / Q000031:analogs & derivatives / Q000494:pharmacology*; D016923:Cell Death / Q000187:drug effects; D005434:Flow Cytometry; D006801:Humans; D015448:Leukemia, B-Cell / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D016399:Lymphoma, T-Cell / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000494:pharmacology*; D011524:Protoporphyrins / Q000096:biosynthesis*; D014407:Tumor Cells, Cultured
chemical_list: D017319:Photosensitizing Agents; D011524:Protoporphyrins; D000622:Aminolevulinic Acid; C028025:protoporphyrin IX; C419924:5-aminolevulinic acid hexyl ester