Characterisation of ISAV proteins from cell culture.
The characterisation of 2 infectious salmon anemia virus (ISAV) proteins is described. Proteins were harvested from ISAV-infected Chinook salmon embryo (CHSE)-214 cell culture by continuous elution denaturing gel electrophoresis, enabling the harvest of specific molecular weight fractions. Through the use of a polyclonal antiserum to ISAV, it was possible to identify a potentially autolytic major antigen of 72 kDa and a glycosylated protein of approximately 38 kDa which varied in size depending on cell line compatibility. N-terminal amino acid sequencing of the glycosylated proteins suggests that it is encoded by segment 6 of the ISAV genome. Further, sequence analysis of the glycosylated protein account for the variable molecular weight and may explain differences in host cell compatibility.
Griffiths
S
S
Molecular Group RPC, Fredericton, New Brunswick, Canada. sgriffit@rpc.unb.ca
Cook
M
M
Mallory
B
B
Ritchie
R
R
D000595:Amino Acid Sequence; D000818:Animals; D018929:Cell Culture Techniques; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D005393:Fish Diseases / Q000821:virology; D006031:Glycosylation; D007106:Immune Sera; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009975:Orthomyxoviridae / Q000737:chemistry* / Q000145:classification / Q000235:genetics; D009976:Orthomyxoviridae Infections / Q000662:veterinary / Q000821:virology; D012474:Salmon / Q000196:embryology; D014764:Viral Proteins / Q000737:chemistry* / Q000235:genetics
D007106:Immune Sera; D014764:Viral Proteins