The anti-human CD21 antibody, BU33, identifies equine B cells.
The number of antibodies for identifying equine B cells is small and the number that react with well-defined epitopes even smaller. The monoclonal antibody, BU33, which is directed against human CD21 (Complement Receptor 2; CR2) was shown to identify (1) follicular lymphocytes in the lymph nodes and spleen of three horses, and (2) a mean of 18 +/- 6% (SEM) of peripheral blood lymphocytes from 10 horses. These findings indicate that the antibody identifies equine B cells and possibly equine CR2 or a related molecule. This study adds to the reagents available for equine research and diagnostic pathology.
Mayall
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Department of Pathology and Infectious Diseases, The Royal Veterinary College, Royal College Street, London NW1 0TU, UK.
Siedek
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Hamblin
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AS
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis*; D001402:B-Lymphocytes / Q000276:immunology*; D015415:Biomarkers; D005434:Flow Cytometry / Q000662:veterinary; D019084:Fluorescent Antibody Technique, Indirect / Q000662:veterinary; D006736:Horses / Q000097:blood / Q000276:immunology*; D006801:Humans; D016130:Immunophenotyping; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology; D017464:Receptors, Complement 3d / Q000032:analysis / Q000276:immunology*; D013154:Spleen / Q000166:cytology / Q000276:immunology
D000911:Antibodies, Monoclonal; D015415:Biomarkers; D017464:Receptors, Complement 3d