Protection of rainbow trout against infectious hematopoietic necrosis virus four days after specific or semi-specific DNA vaccination.
A DNA vaccine against a fish rhabdovirus, infectious hematopoietic necrosis virus (IHNV), was shown to provide significant protection as soon as 4 d after intramuscular vaccination in 2 g rainbow trout (Oncorhynchus mykiss) held at 15 degrees C. Nearly complete protection was also observed at later time points (7, 14, and 28 d) using a standardized waterborne challenge model. In a test of the specificity of this early protection, immunization of rainbow trout with a DNA vaccine against another fish rhabdovirus, viral hemorrhagic septicemia virus, provided a significant level of cross-protection against IHNV challenge for a transient period of time, whereas a rabies virus DNA vaccine was not protective. This indication of distinct early and late protective mechanisms was not dependent on DNA vaccine doses from 0.1 to 2.5 microg.
LaPatra
S E
SE
Research Division, Clear Springs Foods Inc., PO Box 712, Buhl, ID 83316, USA. scottl@clearsprings.com
Corbeil
S
S
Jones
G R
GR
Shewmaker
W D
WD
Lorenzen
N
N
Anderson
E D
ED
Kurath
G
G
D000818:Animals; D003429:Cross Reactions; D004306:Dose-Response Relationship, Immunologic; D005393:Fish Diseases / Q000276:immunology* / Q000517:prevention & control*; D007273:Injections, Intramuscular; D017686:Oncorhynchus mykiss / Q000276:immunology* / Q000821:virology*; D012209:Rhabdoviridae / Q000235:genetics* / Q000276:immunology*; D018353:Rhabdoviridae Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D013997:Time Factors; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000235:genetics; D014765:Viral Vaccines / Q000008:administration & dosage* / Q000235:genetics
D019444:Vaccines, DNA; D014765:Viral Vaccines