title: Prevalence of and risk factors for IgE sensitization to Anisakis simplex in a Spanish population.
abstract: BACKGROUND
  The number of allergic reactions to A. simplex reported in Spain has increased dramatically in the last decade. Nevertheless, there have been no studies of the prevalence of and possible risk factors for IgE sensitization to this parasite, possibly because suitably specific diagnostic methods have only recently become available. The objective was to investigate the prevalence of and risk factors for IgE sensitization to A. simplex in Galicia, a region of northwestern Spain with a population of about 3 million and high average fish consumption (78.5 g/person per day).
  
  
  METHODS
  The study was performed with a random sample of 2801 healthy blood donors distributed in 53 geographic areas, proportional to the density of donors. IgE sensitization to A. simplex was tested by a capture ELISA method that has proved to be the most specific method currently available.
  
  
  RESULTS
  The results showed a total of only 12 positive subjects, of whom five also showed IgG1 sensitization. All positive subjects and 101 randomly selected seronegative subjects were then included in a case-control study of risk factors for sensitization to A. simplex, based on a telephone interview about fish consumption (especially raw and undercooked fish). All seropositive subjects (but only 25% of seronegative subjects) reported consumption of undercooked fish or homemade raw-fish products.
  
  
  CONCLUSIONS
  Our results strongly suggest that sensitization to A. simplex is caused only by live larvae, and not by allergens contained in fish tissues, and that ingestion of homemade boquerones (anchovies [Engraulis encrasicholus] in vinegar), and to a much lesser extent of undercooked fish, are the main risk factors for IgE sensitization to Anisakis in this region.
authors: Laboratorio de Parasitología, Departamento de Microbiología y Parasitología, Universidad de Santiago de Compostela, Spain.
authors: B
authors: B
authors: Valiñas
authors: S
authors: S
authors: Lorenzo
authors: A
authors: A
authors: Eiras
authors: A
authors: A
authors: Figueiras
authors: M L
authors: ML
authors: Sanmartín
authors: F M
authors: FM
authors: Ubeira
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D017163:Anisakis / Q000276:immunology*; D016001:Confidence Intervals; D003430:Cross-Sectional Studies; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D005399:Fishes / Q000276:immunology / Q000469:parasitology; D005512:Food Hypersensitivity / Q000453:epidemiology / Q000209:etiology / Q000469:parasitology; D006801:Humans; D006967:Hypersensitivity / Q000453:epidemiology / Q000276:immunology / Q000469:parasitology; D007114:Immunization; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D015995:Prevalence; D012016:Reference Values; D012307:Risk Factors; D013030:Spain / Q000453:epidemiology
chemical_list: D007073:Immunoglobulin E