Quantitative evaluation of the lengths of homobifunctional protein cross-linking reagents used as molecular rulers.
Homobifunctional chemical cross-linking reagents are important tools for functional and structural characterization of proteins. Accurate measures of the lengths of these molecules currently are not available, despite their widespread use. Stochastic dynamics calculations now provide quantitative measures of the lengths, and length dispersions, of 32 widely used molecular rulers. Significant differences from published data have been found.


BACKGROUND
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Green
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NS
Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095-1569, USA.
Reisler
E
E
Houk
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KN
D000588:Amines / Q000737:chemistry; D003432:Cross-Linking Reagents / Q000737:chemistry*; D008958:Models, Molecular; D008968:Molecular Conformation; D015394:Molecular Structure; D011506:Proteins / Q000737:chemistry*; D013269:Stochastic Processes; D013438:Sulfhydryl Compounds / Q000737:chemistry
D000588:Amines; D003432:Cross-Linking Reagents; D011506:Proteins; D013438:Sulfhydryl Compounds