[Endocrine differentiation in basocellular carcinoma].
Three (propositus) cases of basal cell carcinoma (BCC) showing endocrine differentiation at the immunohistochemical level were studied using reverse transcriptase-polymerase chain reaction (RT-PCR) to determine the presence of mRNA of chromogranin A. Moreover, 20 (consecutive) cases of BCC were studied with immunohistochemistry alone using chromogranin A, synaptophysin, S100 protein, cytokeratin 20, and neuron-specific enolase antibodies (NSE). The three propositus cases of BCC showed positive results when RT-PCR for mRNA of chromogranin A was performed. Eleven out of 20 consecutive cases of BCC were focally positive for chromogranin A antibody. These results confirm the presence of endocrine differentiation in BCC, demonstrated both with immunohistochemistry and with RT-PCR.
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Dipartimento di Oncologia, Sezione di Anatomia, Istologia e Citologia Patologica M. Malpighi, Ospedale Bellaria, Via Altura 3, I-40139 Bologna. Guido.Collina@AUSL.Bologna.it
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Eusebi
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D014408:Biomarkers, Tumor / Q000032:analysis*; D002280:Basal Cell Carcinoma / Q000737:chemistry / Q000235:genetics / Q000473:pathology*; D002454:Cell Differentiation; D053379:Chromogranin A; D002864:Chromogranins / Q000032:analysis* / Q000235:genetics; D015331:Cohort Studies; D006801:Humans; D007124:Immunoenzyme Techniques; D007381:Intermediate Filament Proteins / Q000032:analysis; D053554:Keratin-20; D009363:Neoplasm Proteins / Q000032:analysis*; D010751:Phosphopyruvate Hydratase / Q000032:analysis; D011446:Prospective Studies; D012333:RNA, Messenger / Q000032:analysis; D012334:RNA, Neoplasm / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D009418:S100 Proteins / Q000032:analysis; D012878:Skin Neoplasms / Q000737:chemistry / Q000235:genetics / Q000473:pathology*; D016708:Synaptophysin / Q000032:analysis; D001613:beta 2-Microglobulin / Q000032:analysis / Q000235:genetics
D014408:Biomarkers, Tumor; D053379:Chromogranin A; D002864:Chromogranins; D007381:Intermediate Filament Proteins; C507499:KRT20 protein, human; D053554:Keratin-20; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D009418:S100 Proteins; D016708:Synaptophysin; D001613:beta 2-Microglobulin; D010751:Phosphopyruvate Hydratase