Expression of the apxIV gene in pigs naturally infected with Actinobacillus pleuropneumoniae.
The apxIV gene was detected, by in-situ hybridization with a non-radioactive digoxigenin-labelled probe, in formalin-fixed, paraffin wax-embedded samples of lung tissue from 10 pigs naturally infected with Actinobacillus pleuropneumoniae. A 442 base pair DNA probe of the apxIV gene from A. pleuropneumoniae serotype 2 was generated by the polymerase chain reaction. All 10 pigs infected with A. pleuropneumoniae serotypes 2, 5, 6, or an untypable strain showed a distinct, positive signal in the degenerate alveolar leucocytes in alveolar spaces, and in the dense zone of degenerated cells in granulation tissue surrounding the necrotic areas. Thus, the study demonstrated the presence of the apxIV gene in pleuropneumonic lesions caused by A. pleuropneumoniae.
Cho
W S
WS
Department of Veterinary Pathology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, Suwon 441-744, Kyounggi-Do, Republic of Korea.
Chae
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D000189:Actinobacillus Infections / Q000235:genetics / Q000473:pathology / Q000662:veterinary*; D016977:Actinobacillus pleuropneumoniae / Q000235:genetics / Q000302:isolation & purification*; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D017931:DNA Primers / Q000737:chemistry; D015342:DNA Probes / Q000737:chemistry; D004269:DNA, Bacterial / Q000032:analysis; D015870:Gene Expression; D017403:In Situ Hybridization / Q000662:veterinary; D016676:Macrophages, Alveolar / Q000378:metabolism / Q000473:pathology; D016133:Polymerase Chain Reaction / Q000662:veterinary; D013552:Swine; D013553:Swine Diseases / Q000235:genetics* / Q000473:pathology
C122177:ApxIVA protein, Actinobacillus pleuropneumoniae; D001426:Bacterial Proteins; D017931:DNA Primers; D015342:DNA Probes; D004269:DNA, Bacterial