Structure of a polysaccharide from a Rhizobium species containing 2-deoxy-beta-D-arabino-hexuronic acid.
The structure of the extracellular polysaccharide (EPS) produced by the Rhizobium sp. B strain isolated from atypical nodules on alfalfa has been determined using a combination of chemical and physical techniques (methylation analysis, high pH-anion exchange chromatography (HPAEC), mass spectrometry and 1-D and 2-D NMR spectroscopy). As opposed to the EPS from other strains of Rhizobium, the EPS from the sp. B strain contains D-Glc together with L-Rha and 2-deoxy-D-arabino-hexuronic acid. It is a polymer of a repeating unit having the following structure: --> 4)-beta-D-Glcp-(1 --> 4)-alpha-L-Rhap -(1 --> 3)-beta-D-Glcp-(1 --> 4)-2-deoxy-beta-D-GlcpA-(1 -->. The polysaccharide also contains 0.6 O-acetyl groups per sugar which have not been located.
Guentas
L
L
Département de Génie Biologique, Institut Universitaire de Technologie, Université de Picardie Jules Verne, Amiens, France.
Pheulpin
P
P
Michaud
P
P
Heyraud
A
A
Gey
C
C
Courtois
B
B
Courtois
J
J
D000107:Acetylation; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002852:Chromatography, Ion Exchange; D008401:Gas Chromatography-Mass Spectrometry; D005947:Glucose / Q000032:analysis; D000455:Medicago sativa / Q000382:microbiology; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000302:isolation & purification*; D012210:Rhamnose / Q000032:analysis; D012231:Rhizobium / Q000737:chemistry* / Q000302:isolation & purification
D011135:Polysaccharides, Bacterial; D005947:Glucose; D012210:Rhamnose