title: Accumulation of medium chain acyl-CoAs during beta-oxidation of long chain fatty acid by isolated peroxisomes from rat liver.
abstract: We have reported fatty alcohol synthesis accompanied by chain elongation in liver peroxisomes (Biochim. Biophys. Acta, 1346, 38 (1997)). In the present experiment, we studied what kind of acyl-CoA(s) destined to be utilized as primer for fatty alcohol synthesis accumulate(s) during peroxisomal beta-oxidation. Peroxisomes were prepared from rat liver treated with clofibrate, a peroxisome proliferator, and incubated with [U-14C]palmitate, in order to investigate acyl-CoAs after beta-oxidation. At 1 mM concentration, MgATP activated beta-oxidation, but inhibited beta-oxidation at concentrations higher than 1 mM. After incubation of peroxisomes with palmitate, various acyl-CoAs were formed. Among medium-chain labelled acyl-CoAs, octanoyl-CoA was mainly detected. These results suggest that octanoyl-CoA accumulates during beta-oxidation of palmitate. When peroxisomes were incubated with [9,10-(3)H]palmitate and [9,10-(3)H]stearate, among medium-chain acyl-CoAs, octanoyl-CoA and decanoyl-CoA were primarily detected, respectively, suggesting the occurrence of at least 4 cycles of beta-oxidation of both fatty acids by peroxisomes.
authors: Department of Pathological Biochemistry, Faculty of Pharmaceutical Sciences, Josai University, Sakado, Saitama, Japan.
authors: F
authors: F
authors: Hashimoto
authors: Y
authors: Y
authors: Furuya
authors: H
authors: H
authors: Hayashi
mesh_terms: D000214:Acyl Coenzyme A / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D002994:Clofibrate / Q000008:administration & dosage; D004952:Esters; D008099:Liver / Q000648:ultrastructure*; D008297:Male; D010084:Oxidation-Reduction; D019308:Palmitic Acid / Q000378:metabolism; D020675:Peroxisomes / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013229:Stearic Acids / Q000378:metabolism
chemical_list: D000214:Acyl Coenzyme A; D004952:Esters; D013229:Stearic Acids; D019308:Palmitic Acid; C031183:stearic acid; D000255:Adenosine Triphosphate; D002994:Clofibrate