title: Immunocytochemical localization of a caveolin-1 isoform in human term extra-embryonic membranes using confocal laser scanning microscopy: implications for the complexity of the materno-fetal junction.
abstract: This immunochemical, immunocytochemical, histological and ultrastructural study demonstrates the presence of caveolin 1 in a number of locations in term human extra-embryonic membranes. Strong expression was observed in fetal blood vessel endothelial cells of chorionic villi (cv) and in cv, amniotic and chorionic plate mesenchymal cells, but weak expression was characteristic of trophoblast. Expression in the amniotic epithelium indicated a stronger association with apical as opposed to baso-lateral membranes. Strong immunoreactivity in the thin lining layer of the maternal blood space of the basal plate was a surprising finding. Previously defined as trophoblast, we argue that this is at least partly endothelium based on this new histological, ultrastructural and immunocytochemical data.
authors: Advanced Light Microscope Facility, Department of Pre-Clinical Sciences, Leicester Warwick Medical School, University Road, Leicester, LE1 9HN, UK.
authors: S
authors: S
authors: Byrne
authors: A
authors: A
authors: Cheent
authors: J
authors: J
authors: Dimond
authors: G
authors: G
authors: Fisher
authors: C D
authors: CD
authors: Ockleford
mesh_terms: D000650:Amnion / Q000737:chemistry; D015153:Blotting, Western; D051242:Caveolin 1; D022461:Caveolins / Q000032:analysis*; D004848:Epithelium / Q000737:chemistry; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007150:Immunohistochemistry; D018613:Microscopy, Confocal; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D008871:Microvilli / Q000648:ultrastructure; D010920:Placenta / Q000737:chemistry* / Q000648:ultrastructure; D011247:Pregnancy; D014018:Tissue Distribution; D014327:Trophoblasts / Q000737:chemistry / Q000648:ultrastructure
chemical_list: C495540:CAV1 protein, human; D051242:Caveolin 1; D022461:Caveolins