Isolation of symlandine from the roots of common comfrey (Symphytum officinale) using countercurrent chromatography.
Three pyrrolizidine alkaloids, symlandine, symphytine, and echimidine (1-3), were isolated from the roots of Symphytum officinale using a one-step countercurrent chromatography procedure. The structures of 1-3 were confirmed by several spectroscopic techniques including 2D NMR methods. This is the first description of the separation of symlandine (1) from its stereoisomer, symphytine (2).
Kim
N C
NC
Chemistry and Life Sciences, Research Triangle Institute, 3040 Cornwallis Road, Research Triangle Park, North Carolina 27709, USA.
Oberlies
N H
NH
Brine
D R
DR
Handy
R W
RW
Wani
M C
MC
Wall
M E
ME
D003377:Countercurrent Distribution; D009682:Magnetic Resonance Spectroscopy; D019684:Magnoliopsida / Q000737:chemistry*; D008956:Models, Chemical; D018517:Plant Roots / Q000737:chemistry; D011763:Pyrrolizidine Alkaloids / Q000737:chemistry / Q000302:isolation & purification*
D011763:Pyrrolizidine Alkaloids; C433586:symlandine