A new extraction method for Acinetobacter species ODB-L2 rough form lipopolysaccharide from culture broth.
The Gram-negative bacterium Acinetobacter species ODB-L2 produces lipopolysaccharide (LPS) in culture broth. The LPS could not be purified by conventional extraction methods using 90% phenol/water or 90% phenol/chloroform/petroleum ether mixed solvent. Extraction was achieved employing an admixture of chloroform, ethanol, and 4 M HCI solution. The LPS was purified from dissolving the crude extracts in 90% phenol and LPS sediment formed by addition of methanol. The LPS was characterized by chemical, biochemical, and physicochemical methods as rough form 3-hydroxydodecanoic acid rich LPS.
Kunii
K
K
Takamatsu Technical Research Institute of Shikoku Instrumentation Co, Ltd, Kagawa, Japan.
Nakamura
S
S
Sato
C
C
Fukuoka
S
S
D000150:Acinetobacter / Q000737:chemistry* / Q000145:classification / Q000254:growth & development; D011232:Chemical Precipitation; D003470:Culture Media; D008401:Gas Chromatography-Mass Spectrometry; D007850:Lauric Acids / Q000378:metabolism; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D000432:Methanol / Q000378:metabolism; D010636:Phenols / Q000378:metabolism; D012997:Solvents
D003470:Culture Media; D007850:Lauric Acids; D008070:Lipopolysaccharides; D010636:Phenols; D012997:Solvents; C022145:3-hydroxydodecanoic acid; D000432:Methanol