Use of the ciliated protozoan Tetrahymena pyriformis for the assessment of toxicity and quantitative structure--activity relationships of xenobiotics: comparison with the Microtox test.
Cytotoxicity and quantitative structure-activity relationships of 13 inorganic and 21 organic substances were determined using three bioassays performed on the ciliated protozoan Tetrahymena pyriformis and the luminescent bacterium Vibrio fischeri. The best concordance of toxicity results was observed between the T. pyriformis FDA--esterase activity and population growth inhibition tests for the organic compounds. The sensitivity of these two assays is compared with that of the Microtox test. The T. pyriformis FDA test showed a high sensitivity is most cases. The aim of the current research was to determine whether the relative toxicity of metal ions and organic molecules, with these three bioassays, was predictable using three ion characteristics and hydrophobicity, respectively. For metal ions, the variable that best modeled the toxicity data obtained with the two T. pyriformis tests was the softness index [sigma(p), i.e., (coordinate bond energy of the metal fluoride--coordinate bond energy of the metal iodide)/(coordinate bond energy of the metal fluoride)]. No correlation was found with the Microtox test. For organic compounds, a significant correlation was observed between the hydrophobicity coefficient and the toxicity data. This correlation is closer with the two tests using Tetrahymena.
Bogaerts
P
P
Laboratoire de Biologie Comparée des Protistes, UPRESA 6023, Université Blaise Pascal, 63117 Aubière Cedex, France.
Bohatier
J
J
Bonnemoy
F
F
D000818:Animals; D001419:Bacteria; D004950:Esterases / Q000378:metabolism; D007477:Ions; D008163:Luminescent Measurements; D008670:Metals; D000442:Octanols / Q000737:chemistry; D021281:Quantitative Structure-Activity Relationship; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012995:Solubility; D013769:Tetrahymena pyriformis / Q000187:drug effects* / Q000201:enzymology; D018675:Toxicity Tests; D014481:United States; D014486:United States Food and Drug Administration; D014867:Water / Q000737:chemistry; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D015262:Xenobiotics / Q000737:chemistry / Q000633:toxicity*
D007477:Ions; D008670:Metals; D000442:Octanols; D014874:Water Pollutants, Chemical; D015262:Xenobiotics; D014867:Water; D004950:Esterases