Acetyl- and butyrylcholinesterase molecular forms in normal and streptozotocin-diabetic rat retinal pigment epithelium.
We studied the composition of molecular forms of acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) in normal and streptozotocin-induced diabetic rat retinal pigment epithelium (RPE). Tissues were sequentially extracted with saline (S(1)) and saline-detergent buffers (S(2)). About a 50% decrease in AChE molecular forms was observed in the diabetic RPE compared to the controls. Approximately 70% of the BChE activity in normal RPE was brought into solution and evenly distributed in S(1) and S(2). Analysis of the fractions from RPE revealed the presence of G(A)(1), G(A)(4) and a small proportion of G(H)(4) BChE forms in S(1); whereas G(A)(4) and G(A)(1) molecules predominate in S(2). A 40% decrease in the activity of G(A)(4) in S(2) was observed in the diabetic RPE. Our results show that diabetes caused a remarkable decrease in the activity of cholinesterases molecular forms in the RPE. This might be related to the alterations observed in diabetic retinopathy.
Sánchez-Chávez
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G
Departamento de Neurociencias, Instituto de Fisiología Celular, UNAM Apdo. Postal 70-253, 04510, México DF, Mexico.
Salceda
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R
D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D002091:Butyrylcholinesterase / Q000378:metabolism*; D003921:Diabetes Mellitus, Experimental / Q000201:enzymology*; D005260:Female; D007527:Isoenzymes / Q000378:metabolism*; D010857:Pigment Epithelium of Eye / Q000201:enzymology*; D051381:Rats; D020318:Rats, Long-Evans; D012016:Reference Values
D007527:Isoenzymes; D000110:Acetylcholinesterase; D002091:Butyrylcholinesterase