Comparison of two platelet additive solutions.
The use of an additive solution for substitution of plasma for storage of leukodepleted platelet concentrates can have many advantages. In this study, a comparison was made between two platelet additive solutions: one containing citrate and acetate (PAS-II), the other also supplemented with additional salts such as magnesium, and with gluconate (Composol-PS). Donor-dependent differences were avoided by applying a paired experimental design (n = 10). The platelet concentrates were prepared by pooling five buffy coats and the additive solution, and prestorage filtration was utilized to remove leucocytes to well below 1 x 106. Storage of platelet concentrates up to 9 days after blood collection revealed that platelet concentrates in Composol-PS maintained an almost constant pH of on average 6.93 from day 2 through day 7, and at 6.90 at day 9. This was in contrast to PAS-II, which showed a gradually decreasing pH from on average 6.97 at day 1 to 6.86 at day 9. In all units stored in both solutions the swirling effect was present during 9 days of storage. In conclusion, both additive solutions allow storage of platelets, derived from pooled buffy coats, for up to 9 days after collection of the whole blood, with maintenance of good quality in vitro. Composol-PS has a slightly better buffering capacity, reflected as a more constant pH throughout the storage period.
van der Meer
P F
PF
Blood Bank North Holland, Amsterdam, The Netherlands. bnhams@hetnet.nl
Pietersz
R N
RN
Reesink
H W
HW
D000085:Acetates; D001781:Blood Component Removal; D001792:Blood Platelets; D001793:Blood Preservation; D019343:Citric Acid; D005942:Gluconates; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008274:Magnesium; D012996:Solutions; D013997:Time Factors
D000085:Acetates; D005942:Gluconates; D012996:Solutions; D019343:Citric Acid; D008274:Magnesium; C030691:gluconic acid