Synthesis and nuclease stability of trilysyl dendrimer-oligodeoxyribonucleotide hybrids.
Hybrids of oligonucleotides and trilysyl-dendrimers with terminal acyl groups were prepared via solid-phase synthesis, including a DNA hexamer bearing an additional 3'-appendage. These were shown to be degraded more slowly by nuclease S1 than control strands, particularly at low pH, and, in one case, to form a duplex with a complementary strand whose melting point at pH 7 was higher than that of the control duplex.
Sarracino
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DA
Department of Chemistry, Tufts University, 02155, Medford, MA, USA.
Richert
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C
D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008239:Lysine / Q000737:chemistry*; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry
D009838:Oligodeoxyribonucleotides; D008239:Lysine