Purification and identification of N-glycolylneuraminic acid (Neu5Gc) from the holothuroidea Gumi, Cucumaria echinata.
N-Glycolylneuraminic acid (Neu5Gc), precious sialic acid which could not be synthesized by a chemical method, occurrs in the body of holothuroidea, Gumi Cucumaria echinata. Gumi contains 85% of total sialic acid, as Neu5Gc, in the body. Neu5Gc was purified from dry powder of the body using Dowex 1-x8 (HCOO* form) anion exchange chromatography after mild acid hydrolysis with 0.1 N trifluoroacetic acid. Using GC-MS and 1H-NMR spectroscopy, the purified Neu5Gc was correctly identified to be Neu5Gc. The purity of Neu5Gc was more than 99%. This is the first report of purification and identification of Neu5Gc from holothuroidea by using anion exchange chromatography, GC-MS, and 1H-NMR.
Sumi
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Kyushu National Industrial Research Institute, Tosu, Saga, Japan.
Sallay
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Asakawa
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Park
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Miyazaki
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Ohba
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H
D000818:Animals; D000837:Anion Exchange Resins; D002852:Chromatography, Ion Exchange / Q000379:methods; D002855:Chromatography, Thin Layer; D008401:Gas Chromatography-Mass Spectrometry; D009682:Magnetic Resonance Spectroscopy; D009438:Neuraminic Acids / Q000737:chemistry / Q000302:isolation & purification*; D012117:Resins, Synthetic; D012616:Sea Cucumbers / Q000737:chemistry*
D000837:Anion Exchange Resins; D009438:Neuraminic Acids; D012117:Resins, Synthetic; C032592:N-glycolylneuraminic acid; C027972:Dowex