[Preliminary analysis of genetic characteristics of the nonstructural protein NSP4 of rotavirus strains with different serotypes in China].
OBJECTIVE
To study the genotypes and genetic variations of nonstructural protein NSP4 of rotavirus strains with different serotypes in China.


METHODS
NSP4 cDNA of the four serotypes G1, G2, G3 and G9 were cloned and sequenced by SILVER SEQUENCE DNA Sequencing System.


RESULTS
The identity of the nucleotide and amino acid were 78.8%-93.5%, 83.0%-95.5%, respectively. NSP4 from strains G1, G3, G9 all fell into Wa allele, while that of G9 fell into KUN allele. Some variations of the amino acids were observed localizing at the position of 135-145 in the VP4 binding domain.


CONCLUSIONS
The results indicated that the two alleles Wa and KUN are the major prevalent NSP4 genotypes in China.
Xu
S
S
Institute of Virology, Chinese Academy of Preventive Medicine, Beijing 100052, China.
Wang
J
J
Zhang
Z
Z
D000818:Animals; D002648:Child; D002675:Child, Preschool; D005814:Genes, Viral; D006023:Glycoproteins / Q000145:classification / Q000235:genetics*; D006801:Humans; D012401:Rotavirus / Q000145:classification / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D012703:Serotyping; D014118:Toxins, Biological; D017361:Viral Nonstructural Proteins / Q000145:classification / Q000235:genetics*
D006023:Glycoproteins; C081369:NS28 protein, rotavirus; D014118:Toxins, Biological; D017361:Viral Nonstructural Proteins