Selective separation and simultaneous determination of trace levels of five types of fluorinated quinolone drugs by thin-layer chromatography/fluorescence densitometry.
This paper reports the determination of trace levels of 5 types of fluorinated quinolone drugs, i.e., ciprofloxacin, norfloxacin, enoxacin, pefloxacin, and ofloxacin, by thin-layer chromatography (TLC)/fluorescence densitometry. The new analytical method uses 2-step TLC development, selective separation, and simultaneous determination of the 5 drugs. The method was also applied to the determination of recoveries of standards of the 5 drugs in plasma and urine samples. The results show that the method has a wide linear range, high repeatability, and good stability.
Wang
P L
PL
Shanxi University, Test & Analysis Center, People's Republic of China.
Chen
L
L
Fan
Y F
YF
D000890:Anti-Infective Agents / Q000032:analysis* / Q000097:blood / Q000652:urine; D002138:Calibration; D002855:Chromatography, Thin Layer / Q000379:methods*; D002939:Ciprofloxacin / Q000032:analysis / Q000097:blood / Q000652:urine; D003720:Densitometry; D004355:Drug Stability; D015365:Enoxacin / Q000032:analysis / Q000097:blood / Q000652:urine; D005453:Fluorescence; D009643:Norfloxacin / Q000032:analysis / Q000097:blood / Q000652:urine; D015242:Ofloxacin / Q000032:analysis / Q000097:blood / Q000652:urine; D015366:Pefloxacin / Q000032:analysis / Q000097:blood / Q000652:urine; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D058428:Silica Gel; D012822:Silicon Dioxide
D000890:Anti-Infective Agents; D015366:Pefloxacin; D015365:Enoxacin; D002939:Ciprofloxacin; D058428:Silica Gel; D012822:Silicon Dioxide; D015242:Ofloxacin; D009643:Norfloxacin