[Cloning and sequence analysis of truncated S gene from circulation of patients with chronic hepatitis B virus infection].
OBJECTIVE
To find different mutated status of HBV DNA in circulation from chronic HBV patients.


METHODS
Specially designed primers and polymerase chain reaction method were applied to amplify the whole S gene of HBV from the serum of 2 patients. After being sequenced, 4 clones were compared with HBV adr subtype (China strain) to identify the mutant sites.


RESULTS
Sequencing results implied that there was a truncated large/middle S gene in the serum of the patients. Besides that, HBsAg and HBV DNA polymerase defective clones were also detected.


CONCLUSIONS
Truncated middle S gene is found in the circulation of patients with chronical HBV infection, suggestive of a poor prognosis.
Dong
J
J
Gene Therapy Research Center, Institute of Infectious Diseases, 302nd Hospital of PLA, Beijing 100039, China.
Cheng
J
J
Wang
Q
Q
Wang
G
G
Shi
S
S
Liu
Y
Y
Xia
X
X
Si
C
C
D003001:Cloning, Molecular; D004279:DNA, Viral / Q000097:blood*; D005260:Female; D005814:Genes, Viral; D006514:Hepatitis B Surface Antigens / Q000235:genetics*; D019694:Hepatitis B, Chronic / Q000097:blood / Q000821:virology*; D006801:Humans; D008875:Middle Aged; D011498:Protein Precursors / Q000235:genetics
D004279:DNA, Viral; D006514:Hepatitis B Surface Antigens; D011498:Protein Precursors; C050806:presurface protein 1, hepatitis B surface antigen; C050807:presurface protein 2, hepatitis B surface antigen