Construction of a physical map of an autism susceptibility region in 7q32.3-q33.
The fast evolving progress of the human genome mapping and sequencing efforts facilitate the detection of genes also for complex traits. We focus on the detection of susceptibility loci for autism, a prototypical pervasive developmental disorder. Five genome screens worldwide have identified several putative locations of susceptibility genes thus far, with the most common region on chromosome 7q. In order to identify new candidate genes for infantile autism we constructed a physical map of bacterial artificial chromosome, P1-derived artificial chromosome and yeast artificial chromosome clones of a 3 Mb region between D7S1575 and D7S500, including a complete contig of the approximately 1.2 Mb region around D7S2533, the marker with the most significant association result. We developed 16 novel sequence tag sites and mapped 23 genes/expressed sequence tags to the contigs. As this map contains a putative autistic disorder locus this integrated physical and transcript map provides a valuable resource for identification of candidate gene(s).
Beyer
K S
KS
Department of Molecular Genome Analysis, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, 69120 Heidelberg, Germany.
Klauck
S M
SM
Wiemann
S
S
Poustka
A
A
D001321:Autistic Disorder / Q000235:genetics*; D001435:Bacteriophages / Q000235:genetics; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics; D018244:Chromosomes, Artificial, Yeast / Q000235:genetics; D002897:Chromosomes, Human, Pair 7 / Q000235:genetics*; D004247:DNA / Q000737:chemistry / Q000235:genetics; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D020161:Physical Chromosome Mapping; D017422:Sequence Analysis, DNA; D016324:Sequence Tagged Sites
D004247:DNA