Insulin resistance and type 2 diabetes are not related to resistin expression in human fat cells or skeletal muscle.
Resistin is secreted by rodent fat cells and was recently postulated to be an important link between obesity and insulin resistance. We examined Resistin gene expression with real-time RT-PCR in human isolated fat cells, adipose tissue, and muscle from 42 individuals of varying degrees of overweight and who had normal insulin sensitivity or were insulin-resistant or Type 2 diabetic. Resistin was not expressed in human muscle nor was it expressed in most human isolated fat cells or intact biopsies. No difference was found between normal, insulin-resistant, or Type 2 diabetic samples. However, a very low but specific Resistin expression could be demonstrated in isolated fat cells and intact adipose tissue from some individuals (n = 3 and n = 4, respectively). There was no evidence for the expression of splice variants in the human samples. Thus, Resistin does not seem to be an important link to insulin resistance and Type 2 diabetes in human.
Nagaev
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Lundberg Laboratory for Diabetes Research, Department of Internal Medicine, Blå Stråket 5, Sahlgrenska University Hospital, S-413 45 Göteborg, Sweden. nagaev@medic.gu.se
Smith
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D000273:Adipose Tissue / Q000378:metabolism*; D017398:Alternative Splicing; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000235:genetics*; D014644:Genetic Variation; D006729:Hormones, Ectopic / Q000235:genetics*; D006801:Humans; D055504:Insulin Receptor Substrate Proteins; D007333:Insulin Resistance / Q000235:genetics*; D036341:Intercellular Signaling Peptides and Proteins; D007962:Leukocytes / Q000378:metabolism; D051379:Mice; D008875:Middle Aged; D018482:Muscle, Skeletal / Q000378:metabolism*; D020932:Nerve Growth Factor; D015345:Oligonucleotide Probes; D010750:Phosphoproteins / Q000235:genetics; D011506:Proteins; D052243:Resistin; D020133:Reverse Transcriptase Polymerase Chain Reaction
D017931:DNA Primers; D006729:Hormones, Ectopic; C526292:IRS1 protein, human; D055504:Insulin Receptor Substrate Proteins; D036341:Intercellular Signaling Peptides and Proteins; C526295:Irs1 protein, mouse; D015345:Oligonucleotide Probes; D010750:Phosphoproteins; D011506:Proteins; C499624:RETN protein, human; C486574:RETNLB protein, human; D052243:Resistin; C499623:Retn protein, mouse; C413576:Retnla protein, mouse; D020932:Nerve Growth Factor