Evaluation and optimization of a reusable hollow fiber ultrafilter as a first step in concentrating Cryptosporidium parvum oocysts from water.
Experiments with a small-scale hollow fiber ultrafiltration system (50,000 MWCO) was used to characterize the filtration process and identify conditions that optimize the recovery of Cryptosporidium parvum oocysts from 2 L samples of water. Seeded experiments were conducted using deionized water as well as four environmental water sources (tap, ground, Arkansas river, and Rio Grande river; 0-30.9NTU). Optimal and consistent recovery of spiked oocysts was observed (68-81%), when the membrane was sanitized with a 10% sodium dodecyl sulfate (SDS) solution and then blocked with 5% fetal bovine serum (FBS).
Kuhn
R C
RC
Department of Biology, New Mexico State University, Las Cruces 88003, USA.
Oshima
K H
KH
D000818:Animals; D016785:Cryptosporidium parvum / Q000302:isolation & purification*; D017743:Equipment Reuse; D005618:Fresh Water / Q000469:parasitology*; D006801:Humans; D008567:Membranes, Artificial; D009391:Nephelometry and Turbidimetry; D014462:Ultrafiltration / Q000295:instrumentation / Q000379:methods*; D018508:Water Purification / Q000295:instrumentation / Q000379:methods*
D008567:Membranes, Artificial