Cytokine mRNA expression in mouse colon: IL-15 mRNA is overexpressed and is highly sensitive to a fibre-like dietary component (short-chain fructo-oligosaccharides) in an Apc gene manner.
On the basis of studies using the Min mouse model of colon carcinogenesis, we have recently proposed that a fibre-like food (short-chain fructo-oligosaccharides, sc-FOS) fermented in the colon may stimulate a mechanism of cancer immunosurveillance. In the present paper, we have investigated the expression of cytokines as potential effector molecules. Interleukin (IL-)4, IL-5, IL-13, IL-15 and interferon (INF)-gamma mRNAs were detected by a multi-probe ribonuclease protection assay in C57BL/6J and Min mouse colons. IL-15 mRNA expression was significantly amplified (P=0.01) by the sc-FOS-enriched diet in the colon of Min mice.
Bassonga
E
E
Human Nutrition Research Center of Nantes, U419 INSERM, Institut de Biologie, 9 Quai Moncousu, Nantes Cedex 01, F44035, France.
Forest
V
V
Pierre
F
F
Bornet
F
F
Perrin
P
P
Meflah
K
K
Menanteau
J
J
D000818:Animals; D003106:Colon / Q000276:immunology* / Q000378:metabolism*; D016207:Cytokines / Q000096:biosynthesis; D004032:Diet; D004043:Dietary Fiber / Q000008:administration & dosage*; D017491:Genes, APC; D019409:Interleukin-15 / Q000096:biosynthesis* / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D009844:Oligosaccharides / Q000008:administration & dosage*; D012333:RNA, Messenger / Q000096:biosynthesis*
D016207:Cytokines; D004043:Dietary Fiber; D019409:Interleukin-15; D009844:Oligosaccharides; D012333:RNA, Messenger; C116580:fructooligosaccharide