High yielding one-pot enzyme-catalyzed synthesis of UDP-glucose in gram scales.
Uridine diphosphoglucose is an important cofactor of glucosylating enzymes. A simple and high yielding one-pot enzymatic synthesis of UDPG on a gram scale from glucose via hexokinase, phosphoglucomutase and UDPG pyrophosphorylase (UGPase) is described. Repetitive addition of substrate was used to avoid inhibition of UGPase. The approach allows recovery of active enzymes and their re-use. The synthesis of UDP-[4-(13)C]-glucose on a 0.5 g scale resulted in a final yield of 70% and a purity of >95% after chromatographic purification.
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Department of Pharmaceutical Biology, Institute of Pharmacy, Johannes Gutenberg-University Mainz, Staudinger Weg 5, 55099 Mainz, Germany.
Stöckigt
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J
D002250:Carbon Radioisotopes / Q000737:chemistry; D002384:Catalysis; D005947:Glucose / Q000737:chemistry; D006593:Hexokinase / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D010733:Phosphoglucomutase / Q000378:metabolism; D005957:UTP-Glucose-1-Phosphate Uridylyltransferase / Q000378:metabolism; D014532:Uridine Diphosphate Glucose / Q000138:chemical synthesis*
D002250:Carbon Radioisotopes; D006593:Hexokinase; D005957:UTP-Glucose-1-Phosphate Uridylyltransferase; D010733:Phosphoglucomutase; D005947:Glucose; D014532:Uridine Diphosphate Glucose