Mutation in the DNA gyrase A Gene of Escherichia coli that expands the quinolone resistance-determining region.
In three Escherichia coli mutants, a change (Ala-51 to Val) in the gyrase A protein outside the standard quinolone resistance-determining region (QRDR) lowered the level of quinolone susceptibility more than changes at amino acids 67, 82, 84, and 106 did. Revision of the QRDR to include amino acid 51 is indicated.
Friedman
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Department of Biological Sciences, Hunter College of The City University of New York, New York, New York 10021, USA. friedman@genectr.hunter.cuny.edu
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D042462:4-Quinolones; D000890:Anti-Infective Agents / Q000494:pharmacology*; D027081:DNA Gyrase; D017931:DNA Primers / Q000737:chemistry; D004250:DNA Topoisomerases, Type II / Q000235:genetics*; D004352:Drug Resistance, Microbial / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D009154:Mutation; D016133:Polymerase Chain Reaction; D011487:Protein Conformation
D042462:4-Quinolones; D000890:Anti-Infective Agents; D017931:DNA Primers; D027081:DNA Gyrase; D004250:DNA Topoisomerases, Type II