title: Imprint status of M6P/IGF2R and IGF2 in chickens.
abstract: Genomic imprinting is a method of gene regulation whereby a gene is expressed in a parent-of-origin-dependent fashion; however, it is hypothesized that imprinting should not occur in oviparous taxa such as birds. Therefore, we examined the allelic expression of two genes in the chicken that are reciprocally imprinted in most mammals, mannose 6-phosphate/insulin-like growth factor 2 receptor (M6P/IGF2R) and insulin-like growth factor 2 (IGF2). Single nucleotide polymorphisms were identified in these genes, and cDNA was prepared from several tissues of embryos heterozygous for these polymorphisms. Both alleles of M6P/IGF2R and IGF2 were expressed in all tissues examined by RT-PCR. Since the expression of these genes was independent of the parent from which they were inherited, we conclude that neither M6P/IGF2R nor IGF2 are imprinted in the chicken.
authors.affiliation: Department of Radiation Oncology, Duke University Medical Center, Durham NC 27710, USA.
authors.forename: C M
authors.initials: CM
authors.lastname: Nolan
authors.forename: J K
authors.initials: JK
authors.lastname: Killian
authors.forename: J N
authors.initials: JN
authors.lastname: Petitte
authors.forename: R L
authors.initials: RL
authors.lastname: Jirtle
mesh_terms: D000818:Animals; D002642:Chick Embryo; D018507:Gene Expression Regulation, Developmental; D018392:Genomic Imprinting; D007335:Insulin-Like Growth Factor II / Q000235:genetics*; D008360:Mannosephosphates / Q000235:genetics*; D017527:Receptor, IGF Type 2 / Q000235:genetics*
chemical_list: D008360:Mannosephosphates; D017527:Receptor, IGF Type 2; C027693:mannose-6-phosphate; D007335:Insulin-Like Growth Factor II