Impaired vitamin A-mediated mucosal IgA response in IL-5 receptor-knockout mice.
To clarify actions of vitamin A on mucosal immunity associated with interleukin-5 (IL-5), we examined effects of vitamin A on mucosal IgA level in IL-5 receptor alpha-chain-knockout (IL-5Ralpha(-/-)) mice. Daily supplementation of retinyl acetate (1 mg/mouse) increased Th2 cytokine levels and a number of their positive cells in the small intestinal mucosa of IL-5Ralpha(-/-) mice, as observed in wild-type or IL-5Ralpha(+/-) mice. Wild-type and heterozygous mice increased the IgA level and a number of IgA-containing cells in the mucosa in response to the vitamin A treatment, but not in IL-5Ralpha(-/-) mice. Retinyl acetate increased anti-cholera toxin (CT) IgA level in the mucosa of wild-type mice, improving their survival rate after an exposure to 0.4 mg of CT. However, retinyl acetate failed to induce resistance to CT toxicity in IL-5Ralpha(-/-) mice. Our results suggest that IL-5 may play an important role in an action of vitamin A on mucosal IgA system.
Nikawa
T
T
Department of Nutrition, School of Medicine, University of Tokushima, 3-18-15 Kuramoto-cho, Tokushima 770-8503, Japan. nikawa@nutr.med.tokushima-u.ac.jp
Ikemoto
M
M
Kano
M
M
Tokuoka
K
K
Hirasaka
K
K
Uehara
S
S
Takatsu
K
K
Rokutan
K
K
Kishi
K
K
D000818:Animals; D003433:Crosses, Genetic; D016207:Cytokines / Q000276:immunology; D004224:Diterpenes; D006579:Heterozygote; D018928:Immunity, Mucosal / Q000187:drug effects; D007071:Immunoglobulin A, Secretory / Q000096:biosynthesis*; D007413:Intestinal Mucosa / Q000276:immunology*; D007421:Intestine, Small / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D018345:Mice, Knockout; D021122:Protein Subunits; D018123:Receptors, Interleukin / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D053649:Receptors, Interleukin-5; D000084562:Retinyl Esters; D018418:Th2 Cells / Q000276:immunology; D014801:Vitamin A / Q000031:analogs & derivatives / Q000494:pharmacology*
D016207:Cytokines; D004224:Diterpenes; D007071:Immunoglobulin A, Secretory; D021122:Protein Subunits; D018123:Receptors, Interleukin; D053649:Receptors, Interleukin-5; D000084562:Retinyl Esters; D014801:Vitamin A; C009166:retinol acetate