title: Role of the renal nerves in the control of renin synthesis during different sodium intakes in the rat.
abstract: OBJECTIVE
  The aim of this study was to evaluate the role of the renal nerves in the regulation of renin synthesis in normotensive rats at different sodium balance.
  
  
  METHODS
  Forty-eight male Sprague-Dawley rats were divided in six experimental groups, combining three diets at different NaCl content (normal 0.4%, low 0.04% or high 4.0%), and the surgical, bilateral renal denervation or the sham procedure. After 7 days of dietary treatment, all rats were sacrificed and plasma renin activity (PRA) was measured. Renin messenger RNA (mRNA) levels in the renal cortex were determined by semiquantitative polymerase chain reaction.
  
  
  RESULTS
  PRA was higher in animals fed the low sodium diet compared with those at standard diet, while it was lower in animals fed the high sodium diet. Renal denervation decreased PRA in normal and low sodium groups, while it did not alter the PRA values in the high sodium group. Renin gene expression significantly increased in rats fed with the low sodium diet compared with the standard diet group, and significantly decreased in rats fed the high sodium diet Renal denervation significantly reduced renin mRNA levels in rats receiving the low sodium diet, but did not produce any significant change in normal or high-sodium groups.
  
  
  CONCLUSIONS
  The activation of renin gene expression during sodium depletion in rats is dependent on the presence of the renal nerves, while the suppression of renin gene expression during a sodium load seems to be due to the macula densa mechanism alone.
authors.affiliation: Centro di Fisiologia Clinica e Ipertensione, University of Milan, Ospedale Maggiore--IRCCS, Italy. raffaello.golin@unimi.it
authors.forename: R
authors.initials: R
authors.lastname: Golin
authors.forename: F
authors.initials: F
authors.lastname: Pieruzzi
authors.forename: C
authors.initials: C
authors.lastname: Munforti
authors.forename: G
authors.initials: G
authors.lastname: Busca
authors.forename: A
authors.initials: A
authors.lastname: Di Blasio
authors.forename: A
authors.initials: A
authors.lastname: Zanchetti
mesh_terms: D000818:Animals; D003714:Denervation; D004039:Diet, Sodium-Restricted; D015870:Gene Expression; D005786:Gene Expression Regulation / Q000502:physiology; D007668:Kidney / Q000294:innervation*; D008297:Male; D009424:Nervous System Physiological Phenomena; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012083:Renin / Q000096:biosynthesis* / Q000097:blood / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D012083:Renin