title: Continuous affinity-based selection: rapid screening and simultaneous amplification of bacterial surface-display libraries.
abstract: A new method for continuous biopanning has been developed. We have combined the power of affinity chromatography with the fecundity of bacteria in a unique process that mimics clonal selection. Mixed populations of bacteria were applied to a fermenter containing the immobilized ligand of interest. Bacteria retained in this affinity fermenter were allowed to grow under continuous washout conditions, such that weakly bound organisms were selectively lost. Those initially rare founder bacteria expressing a receptor for the immobilized ligand (R+ve) were thus enriched and amplified simultaneously. From an initial culture containing 1 x 10(10) R-ve cells spiked with fewer than 30 R+ve bacteria (<1 in 10(8)), final ratios of R+ve/R-ve bacteria as high as 1 in 12 were observed, representing an enrichment factor of 55 million-fold. This technology has considerable potential for rapid screening of bacterial surface-display libraries and in facilitating directed-evolution studies.
authors.affiliation: Division of Genomic Medicine, University of Sheffield, Royal Hallamshire Hospital, Sheffield S10 2RX, UK.
authors.forename: D
authors.initials: D
authors.lastname: Patel
authors.forename: S
authors.initials: S
authors.lastname: Vitovski
authors.forename: H J
authors.initials: HJ
authors.lastname: Senior
authors.forename: M D
authors.initials: MD
authors.lastname: Edge
authors.forename: R C
authors.initials: RC
authors.lastname: Hockney
authors.forename: M J
authors.initials: MJ
authors.lastname: Dempsey
authors.forename: J R
authors.initials: JR
authors.lastname: Sayers
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000032:analysis*; D001426:Bacterial Proteins; D002846:Chromatography, Affinity / Q000379:methods*; D004926:Escherichia coli / Q000737:chemistry*; D005285:Fermentation; D008024:Ligands; D008562:Membrane Glycoproteins / Q000032:analysis*; D019151:Peptide Library
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D008024:Ligands; D008562:Membrane Glycoproteins; D019151:Peptide Library; C055764:S-layer proteins