Production of dwarf lettuce by overexpressing a pumpkin gibberellin 20-oxidase gene.
We investigated the effect of overexpressing a pumpkin gibberellin (GA) 20-oxidase gene encoding an enzyme that forms predominantly biologically inactive products on GA biosynthesis and plant morphology in transgenic lettuce (Lactuca sativa cv Vanguard) plants. Lettuce was transformed with the pumpkin GA 20-oxidase gene downstream of a strong constitutive promoter cassette (El2-35S-Omega). The transgenic plants in which the pumpkin gene was detected by polymerase chain reaction were dwarfed in the T(2) generation, whereas transformants with a normal growth phenotype did not contain the transgene. The result of Southern-blot analysis showed that the transgene was integrated as a single copy; the plants segregated three dwarfs to one normal in the T(2) generation, indicating that the transgene was stable and dominant. The endogenous levels of GA(1) and GA(4) were reduced in the dwarfs, whereas large amounts of GA(17) and GA(25), which are inactive products of the pumpkin GA 20-oxidase, accumulated in these lines. These results indicate that a functional pumpkin GA 20-oxidase is expressed in the transgenic lettuce, resulting in a diversion of the normal pathway of GA biosynthesis to inactive products. Furthermore, this technique may be useful for controlling plant stature in other agricultural and horticultural species.
Niki
T
T
National Institute of Floricultural Science, 2-1 Fujimoto, Tsukuba, Ibaraki 305-8519, Japan.
Nishijima
T
T
Nakayama
M
M
Hisamatsu
T
T
Oyama-Okubo
N
N
Yamazaki
H
H
Hedden
P
P
Lange
T
T
Mander
L N
LN
Koshioka
M
M
D019662:Cucurbitaceae / Q000201:enzymology* / Q000235:genetics; D005875:Gibberellins / Q000096:biosynthesis; D018545:Lactuca / Q000187:drug effects / Q000235:genetics / Q000254:growth & development*; D006899:Mixed Function Oxygenases / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D010937:Plant Growth Regulators / Q000096:biosynthesis / Q000494:pharmacology; D030821:Plants, Genetically Modified; D014230:Triazoles / Q000494:pharmacology
D005875:Gibberellins; D010937:Plant Growth Regulators; D014230:Triazoles; D006899:Mixed Function Oxygenases; C089210:gibberellin, 2-oxoglutarate-oxygen oxidoreductase (20-hydroxylating, oxidizing); C092705:uniconazole