title: [Research of status of provirus HIV DNA in non-progressor and progressor of HIV-1 infected persons].
abstract: OBJECTIVE
  This study was designed to investigate the different status of provirus HIV DNA in non-progressor and progressor of HIV-1 infected persons.
  
  
  METHODS
  HIV RNA, HIV DNA were detected with long-distance PCR (LD-PCR), reverse transcription PCR (RT-PCR), Southern blot and molecular hybridization from plasma and peripheral blood mononuclear cells (PBMCs), using primers locating between long terminal repeats LTR(U5)-LTR(R) and so on.
  
  
  RESULTS
  9.1 kb band is full-length HIV DNA genome which was present in all templates of 12 progressors. Deleted HIV DNA was observed in 9 of 12 progressors. Both the full-length and defective. provirus HIV DNA was detected in 5 of the 18 non-progressors. Defective HIV DNA was only detected in the other non-progressors.
  
  
  CONCLUSIONS
  Clinical stages are closely related with both the full-length and the defective HIV DNA.
authors.affiliation: Department of Infectious Diseases, the First Clinic Hospital, Beijing Medical University, 100034 Beijing, China.
authors.forename: X
authors.initials: X
authors.lastname: Xu
authors.forename: L
authors.initials: L
authors.lastname: Chen
authors.forename: Q
authors.initials: Q
authors.lastname: Wang
authors.forename: J C
authors.initials: JC
authors.lastname: Chermann
mesh_terms: D017931:DNA Primers; D004279:DNA, Viral / Q000032:analysis*; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D016133:Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D004279:DNA, Viral