Expression of stress proteins alpha B-crystallin, ubiquitin, and hsp27 in pallido-nigral spheroids of aged rhesus monkeys.
Ubiquitin and alpha B-crystallin belong to a class of proteins which are overexpressed in a variety of human neuropathological conditions associated with increased cellular stress. In this study we have examined the brains of aged rhesus monkeys (Macaca mulatta; n = 10, mean age: 29.7 years) using antibodies against the stress proteins ubiquitin, alpha B-crystallin, and heat shock protein 27 (hsp27). Here, we demonstrate an increased expression of ubiquitin, alpha B-crystallin, and hsp27 in spheroid bodies predominantly localized in the globus pallidus and pars reticulata of the substantia nigra. A portion of the pallido-nigral spheroids also contained ferric iron as highlighted by Perls' staining. On the basis of these findings we advance the hypothesis that expression of ubiquitin, alpha B-crystallin, and hsp27 in pallido-nigral spheroids of aged rhesus monkeys represents a stress response possibly related to increased iron-mediated oxidative stress.
Schultz
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C
Department of Anatomy, Johann Wolfgang Goethe University, 60590, Frankfurt/Main, Germany. schultz@em.uni-frankfurt.de
Dick
E J
EJ
Cox
A B
AB
Hubbard
G B
GB
Braak
E
E
Braak
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H
D000375:Aging / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D003459:Crystallins / Q000032:analysis / Q000096:biosynthesis*; D005260:Female; D005917:Globus Pallidus / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology; D007150:Immunohistochemistry; D007501:Iron / Q000032:analysis; D008253:Macaca mulatta; D008297:Male; D009363:Neoplasm Proteins / Q000032:analysis / Q000096:biosynthesis*; D018384:Oxidative Stress; D006211:Pantothenate Kinase-Associated Neurodegeneration / Q000378:metabolism / Q000473:pathology; D010300:Parkinson Disease / Q000378:metabolism / Q000473:pathology; D013378:Substantia Nigra / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology; D014452:Ubiquitins / Q000032:analysis / Q000096:biosynthesis*
D003459:Crystallins; D009363:Neoplasm Proteins; D014452:Ubiquitins; D007501:Iron