title: POE-PEG-POE triblock copolymeric microspheres containing protein. I. Preparation and characterization.
abstract: Poly(ortho ester) (POE)-poly (ethylene glycol) (PEG) triblock copolymers (POE-PEG-POE) with different PEG contents were synthesised as carriers for controlled protein delivery. POE-PEG-POE microspheres containing bovine serum albumin (BSA) were prepared using a double-emulsion (water-in-oil-in-water) process. In this first paper of a two-part series, we report the fundamentals of the fabrication and characterization of POE-PEG-POE microspheres. Because the triblock copolymer is more hydrophilic than neat poly(ortho ester), the triblock copolymer yields a more stable first emulsion (water-in-oil) and a greater BSA encapsulation efficiency (90% vs. 30%). No BSA is found on POE-PEG-POE microsphere surfaces measured by X-ray photoelectron spectroscopy, while uniform BSA distributions are observed within the microspheres by confocal microscopy. SEM pictures show that an increase in PEG content results in microspheres with a denser cross-section because of a more stable first emulsion and better affinity between the copolymer and water. POE-PEG(20%)-POE suffers significant swelling during the fabrication process and yields the biggest microspheres. However, the POE-PEG(30%)-POE microspheres are much smaller since the dissolution loss of POE-PEG(30%)-POE in the external water phase may be much higher than that of POE-PEG(20%)-POE. The salt concentration in the external water phase significantly affects the morphology of the resultant microspheres. Microspheres with a dense wall are produced when using pure water as the external water phase. Polymer concentration has less impact on BSA encapsulation efficiency but has a considerable effect on microsphere size and morphology. Increasing the concentration of the polyvinyl alcohol emulsifier does not cause an obvious decrease in microsphere size. However, increased BSA loading results in bigger microspheres.
authors.affiliation: Institute of Materials Research and Engineering, No. 3 Research link, Singapore 117602, Singapore. yy-yang@imre.org.sg
authors.forename: Y Y
authors.initials: YY
authors.lastname: Yang
authors.forename: J P
authors.initials: JP
authors.lastname: Wan
authors.forename: T S
authors.initials: TS
authors.lastname: Chung
authors.forename: P K
authors.initials: PK
authors.lastname: Pallathadka
authors.forename: S
authors.initials: S
authors.lastname: Ng
authors.forename: J
authors.initials: J
authors.lastname: Heller
mesh_terms: D000465:Algorithms; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004655:Emulsions; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D008970:Molecular Weight; D010316:Particle Size; D020442:Poloxamer / Q000737:chemistry*; D011142:Polyvinyl Alcohol / Q000737:chemistry; D011506:Proteins / Q000008:administration & dosage / Q000737:chemistry*; D012710:Serum Albumin, Bovine; D012997:Solvents; D014783:Viscosity
chemical_list: D004655:Emulsions; D011506:Proteins; D012997:Solvents; D020442:Poloxamer; D012710:Serum Albumin, Bovine; D011142:Polyvinyl Alcohol