title: Multilayer amniotic membrane transplantation in severe ocular graft versus host disease.
abstract: OBJECTIVE
  To clarify the usefulness of multilayer amniotic membrane transplantation in an unusual case of calcareous corneal degeneration in a patient with graft-versus-host disease.
  
  
  METHODS
  A 20-year-old Caucasian woman had bilateral calcareous corneal degeneration of one year of evolution, secondary to graft-versus-host disease. Treatment for both eyes with topical steroids and antibiotic ointment was not successful. Right eye had a spontaneous corneal perforation, and a three-layer circle amniotic membrane graft was applied to the whole cornea.
  
  
  RESULTS
  During a follow-up period of 20 months we observed stability of the corneal epithelium and stroma. The amniotic membrane-covered area showed rapid epithelization, reduced inflammation and suppressed fibrosis formation.
  
  
  CONCLUSIONS
  Multilayer amniotic membrane transplantation may be considered an alterantive for reconstructing the ocular surface in a patient with severe dry eyes and calcareous corneal degeneration, even with little perforation.
authors.affiliation: Department of Ophthalmology, University Hospital La Fe, Valencia, Spain. cperis@ctv.es
authors.forename: C
authors.initials: C
authors.lastname: Peris-Martínez
authors.forename: J L
authors.initials: JL
authors.lastname: Menezo
authors.forename: M
authors.initials: M
authors.lastname: Díaz-Llopis
authors.forename: J A
authors.initials: JA
authors.lastname: Aviñó-Martínez
authors.forename: A
authors.initials: A
authors.lastname: Navea-Tejerina
authors.forename: P
authors.initials: P
authors.lastname: Risueño-Reguillo
mesh_terms: D000328:Adult; D000650:Amnion / Q000637:transplantation*; D003316:Corneal Diseases / Q000473:pathology / Q000601:surgery*; D003319:Corneal Stroma / Q000473:pathology; D019573:Epithelium, Corneal / Q000473:pathology; D005260:Female; D005500:Follow-Up Studies; D006086:Graft vs Host Disease / Q000473:pathology / Q000601:surgery*; D006801:Humans; D012422:Rupture, Spontaneous; D014792:Visual Acuity