Production of poly(3-hydroxybutyrateco-3-hydroxyvalerate) from cottonseed oil and valeric acid in batch culture of Raistonia sp. strain JC-64.
A Ralstonia sp. strain JC-64 that is capable of accumulating poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (P[3HB-co-3HV]) from cottonseed oil and valeric acid was isolated. By using a high limiting-nitrogen (HLN) mineral medium as the medium for the second stage of the fermentation process and by adding the two carbon sources at different times, a range of copolymers with 12-62 mol% of 3HV were produced from a series of HLN mineral mediums containing different compositions of cottonseed oil and valeric acid by Ralstonia sp. JC-64. The melting temperature (Tm) of polyhydroxybutyrate from cottonseed oil was 174 degrees C and that of P(3HB-co-3HV) with the highest 3HV-mol fraction (62%) was 81 degrees C.
Song
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Department of System Engineering of Materials and Life Science, Faculty of Engineering, Toyama University, Japan.
Zhao
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Ono
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Shimasaki
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Inoue
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D002244:Carbon / Q000494:pharmacology; D003369:Cottonseed Oil / Q000737:chemistry*; D005285:Fermentation; D016946:Gram-Negative Aerobic Rods and Cocci / Q000378:metabolism*; D008049:Lipase / Q000737:chemistry; D010421:Pentanoic Acids / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry* / Q000378:metabolism*; D011108:Polymers / Q000737:chemistry; D013696:Temperature; D000094122:Polyhydroxybutyrates
D002244:Carbon; D003369:Cottonseed Oil; D008049:Lipase; D010421:Pentanoic Acids; D011091:Polyesters; D011108:Polymers; C052620:poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate); D000094122:Polyhydroxybutyrates; C038780:n-pentanoic acid