[Analysis of gag gene subtypes of HIV-1 variants isolated in Russia by comparative assessment of heteroduplex electrophoretic mobility].
Using heteroduplex mobility assay modified for gag gene analysis (HMA-gag), 37 HIV-1 samples previously genotyped by gag and env nucleotide sequencing were studied. It has been demonstrated that both sensitivity and specificity of HMA-gag were 100%. The gag gene region derived from 20 env subtype A HIV-1 isolates was analyzed by this method. AG recombinant, representing a circulating recombinant form of HIV-1 (AGlbNG) was found among five HIV-1 strains isolated from patients infected through heterosexual contacts in Russia. No novel recombinant forms were found among fifteen HIV-1 variants infected from drug users in 7 cities of Russia. The proposed HMA-gag method extends the potentialities of investigating the genetic variability of HIV-1 and in combination with the previously proposed method for env gene is a convenient approach to search for recombinant forms of this virus.
Kazennova
E V
EV
Bobkov
A F
AF
Selimova
L M
LM
Khanina
T A
TA
Pokrovskiĭ
V V
VV
Heyndrickx
L
L
Van der Groen
G
G
D000163:Acquired Immunodeficiency Syndrome / Q000453:epidemiology / Q000821:virology*; D004586:Electrophoresis; D015750:Genes, gag; D014644:Genetic Variation; D016679:Genome, Viral; D015497:HIV-1 / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D016133:Polymerase Chain Reaction; D012426:Russia / Q000453:epidemiology