Isolation and characteristics of trypsin inhibitor from the hepatopancreas of a squid (Todarodes pacificus).
Trypsin inhibitor was purified from the hepatopancreas of squid (Todarodes pacificus). The final inhibitor preparation was nearly homogeneous by SDS-PAGE with an estimated molecular weight of approximately 6300. The squid trypsin inhibitor was acid- and heat-stable, and active against trypsins from the pyloric ceca of starfish (Asterias amurensis) and saury (Cololabis saira) and porcine pancreatic trypsin. Amino acid composition of the squid trypsin inhibitor was compared with other invertebrate trypsin inhibitors. The squid trypsin inhibitor inhibited the autolysis of walleye pollock (Theragra chalcogramma) myofibrillar proteins.
Kishimura
H
H
Graduate School of Fisheries Sciences, Hokkaido University, Hakodate, 041-8611, Hokkaido, Japan. kishi@fish.hokudai.ac.jp
Saeki
H
H
Hayashi
K
K
D000596:Amino Acids / Q000737:chemistry; D000818:Animals; D002845:Chromatography; D049832:Decapodiformes; D004064:Digestive System / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D006358:Hot Temperature; D010940:Plant Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D013552:Swine; D013696:Temperature; D013997:Time Factors; D014357:Trypsin / Q000378:metabolism*; D014361:Trypsin Inhibitors; D000516:alpha-Amylases / Q000037:antagonists & inhibitors
D000596:Amino Acids; D010940:Plant Proteins; D014361:Trypsin Inhibitors; D000516:alpha-Amylases; D014357:Trypsin