Vasoactive intestinal peptide protects guinea-pig detrusor nerves from anoxia/glucopenia injury.
Vasoactive intestinal peptide (VIP) was tested for its capability to protect the intrinsic nerves of guinea-pig urinary bladder from damage due to anoxia/glucopenia and reperfusion. Guinea-pig detrusor strips were mounted for tension recording in small organ baths and the nerves were subjected to electric field stimulation. VIP (0.3 microM) improved significantly the response of strips to electrical field stimulation either during anoxia/glucopenia or thereafter during reperfusion, as compared to untreated tissues. The antioxidant activity of VIP assessed as its capability to scavenge peroxyl radicals during linoleic acid oxidation corresponded to 6.42+/-0.13 pIC(50) M, i.e. close to the concentration proved to protect strips against the anoxic--glucopenic and reperfusion damage.
Pessina
F
F
Istituto di Scienze Farmacologiche, Via E.S. Piccolomini 170, 53100 Siena, Italy.
Kalfin
R
R
Sgaragli
G
G
D000818:Animals; D004305:Dose-Response Relationship, Drug; D004558:Electric Stimulation; D005260:Female; D016166:Free Radical Scavengers / Q000494:pharmacology; D005947:Glucose / Q000172:deficiency*; D006168:Guinea Pigs; D000860:Hypoxia / Q000503:physiopathology*; D066298:In Vitro Techniques; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects / Q000294:innervation / Q000502:physiology; D010545:Peroxides / Q000037:antagonists & inhibitors / Q000378:metabolism; D015427:Reperfusion Injury / Q000517:prevention & control; D001743:Urinary Bladder / Q000187:drug effects* / Q000294:innervation / Q000502:physiology; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology*
D016166:Free Radical Scavengers; D010545:Peroxides; C049375:perhydroxyl radical; D014660:Vasoactive Intestinal Peptide; D005947:Glucose