Protective effects of aspirin and vitamin E (alpha-tocopherol) against copper- and cadmium-induced toxicity.
A 24-h exposure to copper (400 microM, 600 microM) or cadmium (5 microM, 10 microM) significantly reduces the viability of COS-7 cells. A 2-h preincubation with vitamin E does not protect COS-7 cells from copper-induced toxicity, but does protect against cadmium-induced toxicity. Preincubation with aspirin protects cells from both copper- and cadmium-induced toxicity. A combination of aspirin and vitamin E (10 microM and 25 microM, respectively) increases cell viability in copper-exposed cells in a clearly additive manner, while in cadmium-exposed cells the effects are slightly additive. These results indicate that aspirin and vitamin E can protect cells from metal-induced toxicity. Differences in the protective effects of aspirin and vitamin E on copper versus cadmium-induced toxicity may be due to alternative mechanisms of metal toxicity or antioxidant activity.
Mattie
M D
MD
Nicholas School of the Environment, Duke University, Durham, North Carolina 27708, USA.
Freedman
J H
JH
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001241:Aspirin / Q000494:pharmacology*; D019556:COS Cells; D002104:Cadmium / Q000633:toxicity*; D003300:Copper / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D020011:Protective Agents / Q000494:pharmacology; D018675:Toxicity Tests; D014810:Vitamin E / Q000494:pharmacology*
D000975:Antioxidants; D020011:Protective Agents; D002104:Cadmium; D014810:Vitamin E; D003300:Copper; D001241:Aspirin