[Virus-specific proteins of Crimean-Congo hemorrhagic fever].
A variant of Asian strain Hodzha of Crimean-Congo hemorrhagic fever (Hodzha-A), adapted to Vero-E6 culture and yielding high harvest in it, was used for analysis of the synthesis of virus-specific proteins and protein composition of virions. Major virus-specific proteins with mol. weights 110, 83, 78, 55, 57, and 45 kD and minor low-molecular-weight proteins of 42, 36, 23, and 21 kD were detected by immunoprecipitation with hyperimmune ascitic fluid and polyacrylamide gel electrophoresis in infected cell lysates. N-type glycosylation of gp83 and gp78 was shown using tunicamicin. Viral proteins with mol. weights of 83, 78, 55, 57, and 45 kD were detected in infected cell culture fluid and in purified virion preparations after ultracentrifugation in sucrose density gradient. Two proteins with mol. weights 55-57 and about 180 kD were detected in purified nucleocapside preparations.
Gmyl'
L V
LV
Karganova
G G
GG
Smirnova
S E
SE
Lashkevich
V A
VA
D000818:Animals; D002522:Chlorocebus aethiops; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000032:analysis / Q000302:isolation & purification; D003225:Hemorrhagic Fever Virus, Crimean-Congo / Q000378:metabolism*; D006479:Hemorrhagic Fever, Crimean / Q000378:metabolism* / Q000821:virology; D014709:Vero Cells; D014764:Viral Proteins / Q000032:analysis* / Q000302:isolation & purification
D006023:Glycoproteins; D014764:Viral Proteins