title: A positioned nucleosome on the human U6 promoter allows recruitment of SNAPc by the Oct-1 POU domain.
abstract: The human snRNA promoters contain a proximal sequence element (PSE) required for basal transcription and a distal sequence element (DSE) required for activated transcription. The PSE recruits the multisubunit factor SNAPc, whereas the DSE recruits Oct-1. Oct-1 and SNAPc bind cooperatively to DNA when their respective binding sites are moved into proximity through a mechanism that involves a defined protein-protein contact. Here, we show that on the natural U6 promoter, cooperative binding of Oct-1 and SNAPc is mediated by a positioned nucleosome that resides between the DSE and the PSE. This cooperative binding requires the same protein-protein contact as cooperative binding to closely spaced sites on naked DNA and mediates transcription activation.
authors.affiliation: Howard Hughes Medical Institute, Cold Spring Harbor, New York 11724, USA.
authors.forename: X
authors.initials: X
authors.lastname: Zhao
authors.forename: P S
authors.initials: PS
authors.lastname: Pendergrast
authors.forename: N
authors.initials: N
authors.lastname: Hernandez
mesh_terms: D000495:Allosteric Site; D004247:DNA / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D018983:DNA Footprinting; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000378:metabolism*; D006367:HeLa Cells; D051863:Host Cell Factor C1; D006801:Humans; D008836:Micrococcal Nuclease / Q000378:metabolism; D008954:Models, Biological; D008968:Molecular Conformation; D009707:Nucleosomes / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D050811:Octamer Transcription Factor-1; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012342:RNA, Small Nuclear / Q000235:genetics*; D020218:Response Elements / Q000235:genetics; D013698:Templates, Genetic; D014157:Transcription Factors / Q000737:chemistry* / Q000378:metabolism*; D015533:Transcriptional Activation
chemical_list: D004268:DNA-Binding Proteins; C497692:HCFC1 protein, human; D051863:Host Cell Factor C1; D009707:Nucleosomes; D050811:Octamer Transcription Factor-1; C494165:POU2F1 protein, human; D012333:RNA, Messenger; D012342:RNA, Small Nuclear; C106703:SNAPC protein complex, human; D014157:Transcription Factors; C405486:U6 small nuclear RNA; D004247:DNA; D008836:Micrococcal Nuclease