title: Blood digestion in the malaria mosquito Anopheles gambiae: molecular cloning and biochemical characterization of two inducible chymotrypsins.
abstract: The elucidation of digestive processes in the Anopheles gambiae gut leading to the utilization of the blood meal will result in a deeper understanding of the physiology of blood digestion and its impact on parasite-vector interactions. Accordingly, the identification of digestive serine proteases in A. gambiae has implications for the development of alternative strategies for the control of mosquito-borne diseases. We report here on the cDNA and genomic cloning and on the expression analysis of two closely related chymotrypsin genes, Anchym1 and Anchym2. Genomic cloning revealed that Anchym1 and Anchym2, which map on chromosomal division 25D, are clustered in tandem within 6 kb, both genes being interrupted by two short introns. After blood feeding, transcription of Anchym1 and Anchym2 is induced in the midgut epithelium, followed by secretion of the translated products into the midgut lumen where the Anchym1 and Anchym2 zymogens are activated by partial tryptic digestion. The amino-acid residues forming the substrate pocket of Anchym1 and Anchym2 suggested chymotryptic cleavage specificity. This was confirmed by mass spectrometry analysis and Edman degradation sequencing of proteolytic products generated by the recombinant, trypsin-activated Anchym1.
authors.affiliation: Institut de Biologie Moléculaire et Cellulaire 15, Strasbourg, France; Imperial College of Science, Technology and Medicine, Department of Biology, London, UK.
authors.forename: J
authors.initials: J
authors.lastname: Vizioli
authors.forename: F
authors.initials: F
authors.lastname: Catteruccia
authors.forename: A
authors.initials: A
authors.lastname: della Torre
authors.forename: I
authors.initials: I
authors.lastname: Reckmann
authors.forename: H M
authors.initials: HM
authors.lastname: Müller
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000852:Anopheles / Q000502:physiology*; D001769:Blood / Q000378:metabolism*; D002918:Chymotrypsin / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004063:Digestion / Q000502:physiology*; D004790:Enzyme Induction; D017344:Genes, Insect; D007303:Insect Vectors / Q000502:physiology*; D016778:Malaria, Falciparum; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity; D014018:Tissue Distribution
chemical_list: D018076:DNA, Complementary; D002918:Chymotrypsin