[An experimental study of a new route for Acyclovir administration for anti-keratitis therapeutic treatment].
OBJECTIVE
To study the significance of a collagen shield made by centrifugal method that can deliver Acyclovir.


METHODS
28 white rabbits were divided into four groups for observing releasing of Acyclovir into aqueous at 0.5, 1, 3, 5 hours after adding drugs in two different administrative ways, that were putting drug loaded collagen shields on one eye of the rabbit and subconjunctival injection of drug in another eye of the rabbit as control. The drug concentrations in aqueous of rabbit at different times using different administrative routes were measured by high-performance liquid chromatography (HPLC).


RESULTS
At 0.5 hr of drug administration, the Acyclovir concentrations in aqueous of subconjunctival injected rabbits were obviously higher than that in collagen shields loaded rabbits (t = 4.050, P <0.01), but it became not so notable at the time of 1 hr. (t =2.074, P>0.05). At the time of 3 hrs and 5 hrs, the drug concentrations in aqueous of collagen shield loaded eyes of the rabbits not only were higher than that of the subconjunctival injected rabbits (t=4.761, t=4.190, respectively, P <0.01), but also could sustain rather a long time.


CONCLUSIONS
A drug delivering way using collagen shield made by centrifugal method can replace the subconjunctival injection.
Wang
B
B
Department of Ophthalmology, Second Clinical Medical College, Harbin Medical University, Harbin 150086, China.
Teng
W
W
Han
X
X
D000212:Acyclovir / Q000008:administration & dosage* / Q000493:pharmacokinetics; D000287:Administration, Topical; D000818:Animals; D001082:Aqueous Humor / Q000378:metabolism; D003094:Collagen; D004337:Drug Carriers; D004338:Drug Combinations; D016503:Drug Delivery Systems; D007634:Keratitis / Q000188:drug therapy*; D008567:Membranes, Artificial; D011817:Rabbits
D004337:Drug Carriers; D004338:Drug Combinations; D008567:Membranes, Artificial; D003094:Collagen; D000212:Acyclovir