Nannocystis exedens: a potential biocompetitive agent against Aspergillus flavus and Aspergillus parasiticus.
This study examined the potential for controlling toxigenic Aspergillus flavus and Aspergillus parasiticus by biological means using a myxobacterium commonly found in soil. The ability of Nannocystis exedens to antagonize A. flavus ATCC 16875, A. flavus ATCC 26946, and A. parasiticus NRRL 3145 was discovered. Cultures of aflatoxigenic fungi were grown on 0.3% Trypticase peptone yeast extract agar for 14 days at 28 degrees C. When N. exedens was grown in close proximity with an aflatoxigenic mold, zones of inhibition (10 to 20 mm) developed between the bacterium and mold colony. A flattening of the mold colony on the sides nearest N. exedens and general stunting of growth of the mold colony were also observed. When N. exedens was added to the center of the cross-streak of a mold colony, lysis of the colony by the bacterium was observed after 24 h. Microscopic observations revealed that N. exedens grew on spores, germinating spores, hyphae, and sclerotia of the molds. These results indicate that N. exedens may be a potential biocontrol agent against A. flavus and A. parasiticus.
Taylor
W J
WJ
Tennessee Agricultural Experiment Station, Department of Food Science and Technology, Food Safety Center, The University of Tennessee, Knoxville 37919, USA.
Draughon
F A
FA
D000348:Aflatoxins / Q000096:biosynthesis*; D000935:Antifungal Agents / Q000494:pharmacology*; D001230:Aspergillus / Q000187:drug effects* / Q000502:physiology; D001231:Aspergillus flavus / Q000187:drug effects* / Q000502:physiology; D008826:Microbial Sensitivity Tests; D008858:Microscopy, Phase-Contrast; D009231:Myxococcales; D012988:Soil Microbiology
D000348:Aflatoxins; D000935:Antifungal Agents