title: Heparan sulfate and control of cell division: adhesion and proliferation of mutant CHO-745 cells lacking xylosyl transferase.
abstract: We have examined the role of cell surface glycosaminoglycans in cell division: adhesion and proliferation of Chinese hamster ovary (CHO) cells. We used both wild-type (CHO-K1) cells and a mutant (CHO-745) which is deficient in the synthesis of proteoglycans due to lack of activity of xylosyl transferase. Using different amounts of wild-type and mutant cells, little adhesion was observed in the presence of laminin and type I collagen. However, when fibronectin or vitronectin was used as substrate, there was an enhancement in the adhesion of wild-type and mutant cells. Only CHO-K1 cells showed a time-dependent adhesion on type IV collagen. These results suggest that the two cell lines present different adhesive profiles. Several lines of experimental evidence suggest that heparan sulfate proteoglycans play a role in cell adhesion as positive modulators of cell proliferation and as key participants in the process of cell division. Proliferation and cell cycle assays clearly demonstrate that a decrease in the amount of glycosaminoglycans does not inhibit the proliferation of mutant CHO-745 cells when compared to the wild type CHO-K1, in agreement with the findings that both CHO-K1 and CHO-745 cells take 8 h to enter the S phase.
authors.affiliation: Departamento de Bioquímica, Escola Paulista de Medicina, Universidade Federal de São Paulo, Rua 3 de Maio 100, 04044-020 São Paulo SP, Brazil.
authors.forename: C R
authors.initials: CR
authors.lastname: Franco
authors.forename: H A
authors.initials: HA
authors.lastname: Rocha
authors.forename: E S
authors.initials: ES
authors.lastname: Trindade
authors.forename: I A
authors.initials: IA
authors.lastname: Santos
authors.forename: E L
authors.initials: EL
authors.lastname: Leite
authors.forename: S S
authors.initials: SS
authors.lastname: Veiga
authors.forename: H B
authors.initials: HB
authors.lastname: Nader
authors.forename: C P
authors.initials: CP
authors.lastname: Dietrich
mesh_terms: D000818:Animals; D016466:CHO Cells / Q000166:cytology* / Q000502:physiology; D002448:Cell Adhesion / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D003094:Collagen / Q000502:physiology; D006224:Cricetinae; D005109:Extracellular Matrix / Q000502:physiology*; D005353:Fibronectins / Q000502:physiology; D019812:Heparan Sulfate Proteoglycans / Q000502:physiology*; D007797:Laminin / Q000502:physiology; D019096:Vitronectin / Q000502:physiology
chemical_list: D005353:Fibronectins; D019812:Heparan Sulfate Proteoglycans; D007797:Laminin; D019096:Vitronectin; D003094:Collagen