title: [Association of silver granules with elastic fibers in amalgam reaction of mouth mucosa].
abstract: OBJECTIVE
  Discoloration of the oral mucosa due to amalgam may appear histologically merely as brown pigmentation of the fibrous extracellular matrix. It was the aim of these investigations to identify the fibrous component that contains silver granules.
  
  
  METHODS
  Biopsy specimens from seven patients with clinically diagnosed amalgam tattoos were investigated by light and electron microscopy as well as by X-ray microanalysis.
  
  
  RESULTS
  Light microscopy revealed small brown discolored fibers in all specimens; in sections stained with Weigert's resorcinfuchsin, they appeared dark violet. Scanning electron microscopy revealed metallic granules associated with thin fibers; by X-ray microanalysis, they exhibited preferentially peaks for silver and sulfur. Transmission electron microscopy detected only electron-dense particles in elastic fibers.
  
  
  CONCLUSIONS
  With the different morphological methods, silver granules of amalgam tattoos were exclusively detected within elastic fibers. This result indicates that granular brown discoloration of the matrix fibrils is due to silver impregnation of elastic fibers. Therefore, the histopathological diagnosis of amalgam tattoo is possible even in the absence of larger amalgam particles with black appearance.
authors.affiliation: Abteilung Pathologie, Universität Ulm, Albert-Einstein-Allee 11, 89081 Köln.
authors.forename: W
authors.initials: W
authors.lastname: Mohr
authors.forename: E
authors.initials: E
authors.lastname: Görz
mesh_terms: D001706:Biopsy; D003723:Dental Amalgam / Q000009:adverse effects* / Q000032:analysis; D004547:Elastic Tissue / Q000473:pathology*; D004577:Electron Probe Microanalysis; D005549:Foreign-Body Reaction / Q000473:pathology*; D006801:Humans; D002479:Inclusion Bodies / Q000473:pathology*; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D009061:Mouth Mucosa / Q000473:pathology*; D010859:Pigmentation Disorders / Q000473:pathology*; D012834:Silver / Q000032:analysis*
chemical_list: D012834:Silver; D003723:Dental Amalgam