title: Transcriptional regulation of laccase and cellulase genes during growth and fruiting of Lentinula edodes on supplemented sawdust.
abstract: Transcription of laccase and cellulase genes of Lentinula edodes was examined during growth and development under different temperature and moisture levels on a sawdust-based substrate. RNA was extracted from samples of mycelium and fruit bodies at various stages of development and gene expression was determined by competitive RT-PCR. The level of laccase transcripts was maximal during the mycelial growth stage, and then declined rapidly at the fruiting stage. In contrast, the cellulase transcript level peaked at the veil-break stage during fruit body development. Gene expressions for laccase and cellulase were triggered by microclimatic changes, specifically lower temperature and osmotic pressure.
authors.affiliation: Department of Forest and Forest Products Science, Faculty of Agriculture, Kyushu University, Fukuoka, Japan. ohgasfor@mbox.nc.kyushu-u.ac.jp
authors.forename: S
authors.initials: S
authors.lastname: Ohga
authors.forename: D J
authors.initials: DJ
authors.lastname: Royse
mesh_terms: D002480:Cellulase / Q000235:genetics* / Q000378:metabolism; D003470:Culture Media; D015966:Gene Expression Regulation, Fungal; D042845:Laccase; D020074:Lentinula / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D009997:Osmotic Pressure; D010088:Oxidoreductases / Q000235:genetics* / Q000378:metabolism; D012331:RNA, Fungal / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013696:Temperature; D014158:Transcription, Genetic; D014934:Wood
chemical_list: D003470:Culture Media; D012331:RNA, Fungal; D012333:RNA, Messenger; D010088:Oxidoreductases; D042845:Laccase; D002480:Cellulase