Fungal flora on board the Mir-Space Station, identification by morphological features and ribosomal DNA sequences.
This report is on the morphological and molecular biological identification, using 18S- and ITS1-rDNA sequences, of the "space fungi" isolated on board the Russian Mir-Space Station as the major constituents of the fungal flora. The six fungal strains were isolated from air by using an air sampler or from condensation. Strains were identified as Penicillium chrysogenum, Aspergillus versicolor, or Penicillium sp. by both methods. The species of space fungi were common saprophytic fungi in our living environment, potential pathogens, and allergens. This study concluded that the environment on board the space station Mir allows the growth of potentially pathogenic fungi as true in residential areas on the earth. Therefore, to prevent infection or other health disorders caused by these fungi, easy and reliable methods should be established to survey the fungal flora in a space station.
Makimura
K
K
Teikyo University Institute of Medical Mycology, Hachioji, Tokyo, Japan. makimura@main.teikyo-u.ac.jp
Hanazawa
R
R
Takatori
K
K
Tamura
Y
Y
Fujisaki
R
R
Nishiyama
Y
Y
Abe
S
S
Uchida
K
K
Kawamura
Y
Y
Ezaki
T
T
Yamaguchi
H
H
D001230:Aspergillus / Q000235:genetics / Q000302:isolation & purification*; D015169:Colony Count, Microbial; D004271:DNA, Fungal / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000235:genetics / Q000302:isolation & purification; D018504:Ecological Systems, Closed; D004784:Environmental Monitoring; D005118:Extraterrestrial Environment; D006801:Humans; D010407:Penicillium / Q000235:genetics / Q000302:isolation & purification*; D010408:Penicillium chrysogenum / Q000235:genetics / Q000302:isolation & purification*; D013026:Space Flight; D018531:Spacecraft
D004271:DNA, Fungal; D004275:DNA, Ribosomal