title: Activation by gene amplification of pitB, encoding a third phosphate transporter of Escherichia coli K-12.
abstract: Two systems for the uptake of inorganic phosphate (P(i)) in Escherichia coli, PitA and Pst, have been described. A revertant of a pitA pstS double mutant that could grow on P(i) was isolated. We demonstrate that the expression of a new P(i) transporter, PitB, is activated in this strain by a gene amplification event.
authors.affiliation: Department of Molecular Microbiology and Institute of Biomembranes, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands.
authors.forename: S M
authors.initials: SM
authors.lastname: Hoffer
authors.forename: P
authors.initials: P
authors.lastname: Schoondermark
authors.forename: H W
authors.initials: HW
authors.lastname: van Veen
authors.forename: J
authors.initials: J
authors.lastname: Tommassen
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D029968:Escherichia coli Proteins; D005784:Gene Amplification; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D016296:Mutagenesis; D033902:Periplasmic Binding Proteins; D028044:Phosphate-Binding Proteins; D010710:Phosphates / Q000378:metabolism*
chemical_list: D018528:ATP-Binding Cassette Transporters; D001426:Bacterial Proteins; D002352:Carrier Proteins; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D033902:Periplasmic Binding Proteins; D028044:Phosphate-Binding Proteins; D010710:Phosphates; C090133:PstS protein, E coli