title: A fast method to estimate kinetic constants for enzyme inhibitors.
abstract: We present a method to determine the reaction type and kinetic constants for enzyme inhibitors that decreases the number of experimental assays by at least a factor of five. It is based on a new theoretical formalism in terms of concentrations that dismisses the requirement of estimating initial velocities. Expressions for the time evolution of the concentrations of all the reactants are also given.
authors.affiliation: Centre for Mathematical Biology, Mathematical Institute, Oxford UK. schnell@maths.ox.ac.uk
authors.forename: S
authors.initials: S
authors.lastname: Schnell
authors.forename: C
authors.initials: C
authors.lastname: Mendoza
mesh_terms: D000465:Algorithms; D000818:Animals; D004791:Enzyme Inhibitors / Q000493:pharmacokinetics*; D006801:Humans; D007700:Kinetics; D016018:Least-Squares Analysis; D017711:Nonlinear Dynamics; D013379:Substrate Specificity
chemical_list: D004791:Enzyme Inhibitors