title: Distribution of ecotropic retrovirus receptor protein in rat brains detected by immunohistochemistry.
abstract: Friend murine leukaemia virus (FrMLV) FrC6 clone A8 causes spongiform degeneration in the central nervous system (CNS) of newborn but not 3-week-old rats. To assess whether expression of the ecotropic MLV receptor (CAT-1) in the CNS correlates with the pathogenicity of the A8 virus, we generated an anti-CAT-1 antibody raised against a synthetic peptide that corresponds to the carboxyl-terminal amino acid sequence of CAT-1. In the CNS of newborn and 3 to 4-week-old rats, a strong immunoreactivity against the antibody was detected in most of the endothelial cells. However, almost no expression of CAT-1 was detected in the CNS of 21-week-old rats. In newborn rats, many parenchymal cells in the brain as well as the vascular wall expressed CAT-1 antigen. These findings suggest that retrovirus receptor-bearing glial cells contribute to the neuropathogenesis of MLV, including clone A8, which induces spongiosis in rats only when inoculated into newborns.
authors.affiliation: Institute of Life Science, Soka University, Tangi-cho 1-236, Hachioji, Tokyo 192-8577, Japan1.
authors.forename: Sayaka
authors.initials: S
authors.lastname: Takase-Yoden
authors.affiliation: Institute of Life Science, Soka University, Tangi-cho 1-236, Hachioji, Tokyo 192-8577, Japan1.
authors.forename: Rihito
authors.initials: R
authors.lastname: Watanabe
mesh_terms: D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D000831:Animals, Newborn; D000914:Antibodies, Viral; D001921:Brain / Q000098:blood supply / Q000821:virology*; D004727:Endothelium / Q000821:virology; D005622:Friend murine leukemia virus / Q000276:immunology* / Q000302:isolation & purification; D007106:Immune Sera; D007150:Immunohistochemistry; D007942:Leukemia, Experimental / Q000821:virology; D008562:Membrane Glycoproteins / Q000032:analysis / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D009457:Neuroglia / Q000378:metabolism; D011506:Proteins / Q000138:chemical synthesis / Q000235:genetics / Q000276:immunology; D051381:Rats; D011991:Receptors, Virus / Q000032:analysis / Q000378:metabolism*; D012192:Retroviridae Infections / Q000821:virology; D014412:Tumor Virus Infections / Q000821:virology
chemical_list: D000914:Antibodies, Viral; D007106:Immune Sera; D008562:Membrane Glycoproteins; D011506:Proteins; D011991:Receptors, Virus; C070506:ecotropic murine leukemia virus receptor