title: Gel sequestration of heavy metals by Klebsiella oxytoca isolated from iron mat.
abstract: The enterobacterium Klebsiella oxytoca strain BAS-10 was isolated from sediments under an iron mat formed in a stream receiving leached waters from pyrite mine tailings. Under anaerobic laboratory conditions, BAS-10 fermented Fe(III)-citrate and Na-citrate giving CH(3)COOH and CO(2). In the presence of ferric citrate, BAS-10 secreted quantities of a thick gel containing glucose and/or mannose, if not other sugars. Sugar residues were observed in microbial aggregates using the sugar-specific concanavalin A lectin conjugated with fluorescein and imaged by a scanning confocal laser microscope. The gel bound Fe(III) which quickly precipitated. During fermentation, however, half the initial Fe(III) concentration was reduced to Fe(II) which did not bind to the gel and remained in solution. BAS-10 showed a high tolerance to heavy metals. Its growth was not inhibited by 1 mM Zn-, Pb- or Cd-acetate. These cations also co-precipitated with the iron gel, suggesting a possible application of this strain for abatement of toxic metals under anaerobic conditions.
authors.forename: F
authors.initials: F
authors.lastname: Baldi
authors.forename: A
authors.initials: A
authors.lastname: Minacci
authors.forename: M
authors.initials: M
authors.lastname: Pepi
authors.forename: A
authors.initials: A
authors.lastname: Scozzafava