title: Summary of the first round analyses of the Third International Workshop on Swine Leukocyte Differentiation Antigens.
abstract: The reactivity of 155 monoclonal antibodies submitted to the Third International Workshop on Swine Leukocyte Differentiation Antigens, together with 41 internal standards, was analysed by flow cytometry on 29 different pig cell targets as well as two human cell targets as a means of establishing suitable panels of monoclonal antibodies for more detailed clustering analyses by the various subsections of the workshop. Results were collected either without further gating, with gating based on FS/SS characteristics or with gating based on the co-expression of a reference antibody in two-colour flow cytometry. The CD or SWC reactivity of the internal standards had been established in previous workshops. Data sets were subsequently analysed by statistical clustering using the Leucocyte Typing Database IV software. The resulting 18 cluster groups were allocated to the appropriate second round sections of the workshop, after reviewing the overall cellular reactivity of each cluster as well as the specificity of known standards which clustered in a group.
authors.affiliation: Division of Molecular and Cellular Biology, University of Bristol, Langford BS40 5DU, UK. karin.haverson@bristol.ac.uk
authors.forename: K
authors.initials: K
authors.lastname: Haverson
authors.forename: A
authors.initials: A
authors.lastname: Saalmüller
authors.forename: Z
authors.initials: Z
authors.lastname: Chen
authors.forename: C A
authors.initials: CA
authors.lastname: Huang
authors.forename: A
authors.initials: A
authors.lastname: Simon
authors.forename: J
authors.initials: J
authors.lastname: Seebach
authors.forename: W J
authors.initials: WJ
authors.lastname: Boersma
authors.forename: R
authors.initials: R
authors.lastname: Zwart
authors.forename: T A
authors.initials: TA
authors.lastname: Niewold
authors.forename: E
authors.initials: E
authors.lastname: Thacker
authors.forename: D
authors.initials: D
authors.lastname: Llanes
authors.forename: J M
authors.initials: JM
authors.lastname: de la Lastra
authors.forename: H
authors.initials: H
authors.lastname: Engelhardt
authors.forename: A
authors.initials: A
authors.lastname: Ezquerra
authors.forename: F
authors.initials: F
authors.lastname: Alonso
authors.forename: J
authors.initials: J
authors.lastname: Dominguez
authors.forename: J A
authors.initials: JA
authors.lastname: Ledbetter
authors.forename: L
authors.initials: L
authors.lastname: Grosmaire
authors.forename: R
authors.initials: R
authors.lastname: Lee
authors.forename: J
authors.initials: J
authors.lastname: Nielsen
authors.forename: H
authors.initials: H
authors.lastname: Salmon
authors.forename: I
authors.initials: I
authors.lastname: Valpotic
authors.forename: L
authors.initials: L
authors.lastname: Sver
authors.forename: G
authors.initials: G
authors.lastname: Lackovic
authors.forename: A
authors.initials: A
authors.lastname: Summerfield
authors.forename: K V
authors.initials: KV
authors.lastname: Khanna
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D006801:Humans; D007962:Leukocytes / Q000276:immunology*; D013552:Swine / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD