Ex vivo expansion and engraftment potential of cord blood-derived CD34+ cells in NOD/SCID mice.
For more than 10 years umbilical cord blood (UCB) has been used as an alternative source of hematopoietic stem cells for allogeneic transplantation. Although the clinical results are encouraging, UCB is associated with delayed engraftment. To address these issues we have used the NOD/SCID mouse model to study the engraftment potential of cord blood cells in more detail, to assess the engraftment potential of expanded megakaryocytic progenitor cells, and to study the effect of co-transplantation of mesenchymal stem cells on engraftment.
Fibbe
W E
WE
Department of Hematology, Leiden University Medical Center, Leiden, The Netherlands.
Noort
W A
WA
Schipper
F
F
Willemze
R
R
D000818:Animals; D018952:Antigens, CD34 / Q000032:analysis; D001773:Blood Cells / Q000637:transplantation; D016026:Bone Marrow Transplantation; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured / Q000637:transplantation; D005312:Fetal Blood / Q000166:cytology*; D016332:Fetal Tissue Transplantation; D006085:Graft Survival; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods / Q000639:trends; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000187:drug effects; D006801:Humans; D008168:Lung / Q000166:cytology / Q000196:embryology; D008533:Megakaryocytes / Q000166:cytology; D008648:Mesoderm / Q000166:cytology; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D009928:Organ Specificity; D011123:Polyploidy; D011828:Radiation Chimera; D016511:Severe Combined Immunodeficiency / Q000628:therapy; D013926:Thrombopoietin / Q000494:pharmacology; D014183:Transplantation, Heterologous
D018952:Antigens, CD34; D013926:Thrombopoietin