Trehalose-producing operon treYZ from Arthrobacter ramosus S34.
Arthrobacter ramosus S34, which produces trehalose from maltooligosaccharide, was isolated. A trehalose-producing operon, treYZ, was cloned from the genome. Expression experiments with treY and treZ confirmed that they coded malto-oligosyltrehalose synthase and malto-oligosyltrehalose trehalohydrolase, respectively. The amino acid sequence of TreY from A. ramosus S34 and that from Arthrobacter sp. Q36 did not show high identity, nor did those of TreZ.
Yamamoto
T
T
Hayashibara Biochemical Laboratories, Inc., Okayama, Japan. amaseken@hayashibara.co.jp
Maruta
K
K
Watanabe
H
H
Yamashita
H
H
Kubota
M
M
Fukuda
S
S
Kurimoto
M
M
D000595:Amino Acid Sequence; D001173:Arthrobacter / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D003001:Cloning, Molecular; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009876:Operon / Q000235:genetics; D015183:Restriction Mapping; D014199:Trehalose / Q000096:biosynthesis* / Q000235:genetics*
D014199:Trehalose