title: Characterization of clotting factor IX in plasma-derived preparations by electrophoretic techniques.
abstract: Clotting factor IX preparations from human plasma (pdFIX) have been characterized using electrophoretic methods like sodium dodecyl sulfate-polyacrylamide gel electrophoresis, isoelectric focusing and two-dimensional polyacrylamide gel electrophoresis. Factor IX prior to and after activation with factor XIa was separated by one- and two-dimensional polyacrylamide gel electrophoresis and on isoelectric focusing gels. The main differences between the band patterns of the two pdFIX preparations are due to their purity. Vitronectin was identified by immunological techniques as major accompanying plasma protein, separated from factor IX and characterized by isoelectric focusing and two-dimensional polyacrylamide gel electrophoresis.
authors.affiliation: Octapharma Pharmazeutika Produktionsges.mbH, Vienna, Austria. katharina.pock@octapharma.at
authors.forename: K
authors.initials: K
authors.lastname: Pock
authors.forename: A
authors.initials: A
authors.lastname: Buchacher
authors.forename: A
authors.initials: A
authors.lastname: Rizzi
authors.forename: D
authors.initials: D
authors.lastname: Josić
mesh_terms: D000595:Amino Acid Sequence; D001769:Blood; D015153:Blotting, Western; D002850:Chromatography, Gel; D015180:Electrophoresis, Gel, Two-Dimensional; D005164:Factor IX / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing
chemical_list: D005164:Factor IX