Concurrent maltodextrin and cellodextrin synthesis by Fibrobacter succinogenes S85 as identified by 2D NMR spectroscopy.
1D and 2D NMR experiments were used to analyse the synthesis of various metabolites by resting cells of Fibrobacter succinogenes S85 when incubated with [1-(13)C]glucose, in both extracellular and cellular media. Besides the expected glycogen, succinate, acetate, glucose-1-P and glucose-6-P, maltodextrins and cellodextrins were detected. Maltodextrins were excreted into the external medium. They were found to have linear structures with a maximum degree of polymerization (DP) of about 6 or 7 units. Cellodextrins were located in the cells (cytoplasm and/or periplasm), and their DP was < or = 4. Both labelled (1-(13)C and 6-(13)C) and unlabelled maltodextrins and cellodextrins were detected, showing the contribution of carbohydrate cycling in F. succinogenes, including the reversal of glycolysis and the futile cycle of glycogen. The mechanisms of these oligosaccharide syntheses are discussed.
Matulova
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Laboratoire de Synthèse, Electrosynthèse et Etude de Systèmes à Intérêt Biologique, UMR 6504, Université Blaise Pascal, CNRS, Aubière, France. amdelort@chimpt.uni-bpclermont.fr
Delort
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AM
Nouaille
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Gaudet
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Forano
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D000818:Animals; D001419:Bacteria / Q000378:metabolism*; D002482:Cellulose / Q000031:analogs & derivatives* / Q000096:biosynthesis*; D003470:Culture Media; D003912:Dextrins / Q000096:biosynthesis*; D005947:Glucose / Q000378:metabolism*; D008320:Maltose; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods; D011134:Polysaccharides / Q000096:biosynthesis*; D012417:Rumen / Q000382:microbiology
D003470:Culture Media; D003912:Dextrins; D011134:Polysaccharides; D008320:Maltose; C008315:maltodextrin; D002482:Cellulose; C068994:cellodextrin; D005947:Glucose