title: Regulatory role of IL-1beta in the expression of IL-6 and IL-8 in human corneal epithelial cells during Pseudomonas aeruginosa colonization.
abstract: Pseudomonas aeruginosa is a virulent pathogen and is frequently associated with bacterial keratitis. Recent studies have shown that high levels of interleukin (IL)-1beta and macrophage inflammatory protein-2 are associated with the severity of corneal infection. Interleukin-1beta is a principal inflammatory mediator. Understanding the regulatory role of IL-1beta would provide better understanding of host responses during P. aeruginosa corneal infection. A human corneal epithelial (HCE) cell line and three P. aeruginosa strains were used in this experiment. Confluent HCE cells were challenged with P. aeruginosa and monoclonal antihuman IL-1beta antibody (IL-1beta mAb). The culture supernatants were collected for measuring cytotoxicity and protein levels of IL-1beta, IL-8 and IL-6 by enzyme-linked immunosorbent assay. Results showed that HCE cells expressed low levels of IL-1beta and high levels of IL-6 and IL-8 during P. aeruginosa colonization. Paer1-colonized HCE cells produced higher levels of IL-1beta, IL-6 and IL-8 protein compared to those produced by 6206- and 6294-colonized HCE cells. Administration of IL-1beta mAb decreased the production of IL-8 and IL-6. In conclusion, P. aeruginosa-colonized HCE cells produced low levels of IL-1beta and high levels of IL-6 and IL-8. Neutralizing IL-1beta protein significantly downregulated the production of IL-8 and IL-6.
authors.affiliation: Cooperative Research Centre For Eye Research and Technology, The University of New South Wales, Sydney, Australia. mxue@cclru.randwick.unsw.edu.au
authors.forename: M L
authors.initials: ML
authors.lastname: Xue
authors.forename: M D
authors.initials: MD
authors.lastname: Willcox
authors.forename: A
authors.initials: A
authors.lastname: Lloyd
authors.forename: D
authors.initials: D
authors.lastname: Wakefield
authors.forename: A
authors.initials: A
authors.lastname: Thakur
mesh_terms: D002460:Cell Line; D002470:Cell Survival; D003315:Cornea / Q000166:cytology / Q000378:metabolism / Q000382:microbiology*; D004797:Enzyme-Linked Immunosorbent Assay; D004847:Epithelial Cells / Q000378:metabolism / Q000382:microbiology*; D006801:Humans; D007375:Interleukin-1 / Q000378:metabolism*; D015850:Interleukin-6 / Q000096:biosynthesis*; D016209:Interleukin-8 / Q000096:biosynthesis*; D011550:Pseudomonas aeruginosa / Q000254:growth & development*
chemical_list: D007375:Interleukin-1; D015850:Interleukin-6; D016209:Interleukin-8