title: Purification and substrate specificity of transglutaminases from blood and Streptoverticillium mobaraense.
abstract: A procedure for a fast and simple purification of bovine plasma transglutaminase was developed, which resulted in a homogeneous enzyme preparation. Two different procedures were developed for the purification of pig erythrocyte transglutaminase, both of which resulted in partial purification. Both enzymes were used in cross-linking reactions of alpha-lactalbumin, beta-lactoglobulin, bovine serum albumin, casein, hemoglobin, glycinin, and myosin. The substrate specificity was compared to that of bacterial transglutaminase isolated from Streptoverticillium mobaraense. The bacterial transglutaminase caused cross-linking of a wider range of proteins and, thus, exhibited a lower substrate specificity than the blood transglutaminases. In addition, differences exist in the necessity of the addition of reducing agents. These differences allow specific applications of blood and bacterial transglutaminases at protein cross-linking in single or complex protein systems.
authors.affiliation: TNO Nutrition and Food Research Institute, 3700 AJ Zeist, The Netherlands. A.deJong@voeding.tno.nl
authors.forename: G A
authors.initials: GA
authors.lastname: de Jong
authors.forename: G
authors.initials: G
authors.lastname: Wijngaards
authors.forename: H
authors.initials: H
authors.lastname: Boumans
authors.forename: S J
authors.initials: SJ
authors.lastname: Koppelman
authors.forename: M
authors.initials: M
authors.lastname: Hessing
mesh_terms: D000818:Animals; D002417:Cattle / Q000097:blood; D003429:Cross Reactions; D004912:Erythrocytes / Q000201:enzymology*; D013306:Streptomycetaceae / Q000201:enzymology*; D013379:Substrate Specificity; D013552:Swine / Q000097:blood; D011503:Transglutaminases / Q000302:isolation & purification*
chemical_list: D011503:Transglutaminases