title: Complete sequencing of mono-deprotonated peptide nucleic acids by sustained off-resonance irradiation collision-induced dissociation.
abstract: Complete peptide nucleic acids (PNAs) sequence information is obtained from the unimolecular decomposition of singly-charged PNA oligomers in the negative-ion mode using electrospray ionization coupled with Fourier transform ion cyclotron resonance mass spectrometry (ESI-FTICR-MS) and sustained off-resonance irradiation collision induced dissociation. The 4-mers, n-CATT-c, n-AGCT-c, n-AACT-c, and n-acetylated-AACT-c and two 6-mers, n-AAAAAA-c and n-CCCCCC-c, were investigated to explore the unimolecular decomposition of mixed-nucleobase and homopolymer PNAs representing purine and pyrimidine oligomers, respectively. PNA decomposition is explored using a product-ion appearance curve and double resonance experiments. A decomposition mechanism for sequence ion formation (PNA amide bond cleavage) is proposed.
authors.affiliation: Department of Chemistry, Virginia Commonwealth University, Richmond 84206, USA.
authors.forename: J W
authors.initials: JW
authors.lastname: Flora
authors.forename: D C
authors.initials: DC
authors.lastname: Muddiman
mesh_terms: D002138:Calibration; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D013058:Mass Spectrometry; D020135:Peptide Nucleic Acids / Q000737:chemistry*; D017422:Sequence Analysis, DNA
chemical_list: D020135:Peptide Nucleic Acids