[Detection of Coxsackie B virus IgM antibody in myocarditis patients by Mac ELISA].
OBJECTIVE
To detect Coxsackie B virus type 1-6 IgM antibodies from 193 clinically diagnosed myocarditis cases.


METHODS
The capture IgM ELISA (Mac ELISA) test was used.


RESULTS
The total positive rate of Coxsackie virus B 1-6 IgM antibody in myocarditis patients was 69.6% (135 cases), among which Cox B3 type was as high as 40% (54 cases) of the total positive patients. The others were 29.6% (40 cases), 18.5% (25 cases), 7.4%(10 cases), 3.0% (4 cases), 1.5% (2 cases) of Cox B2, Cox B4, Cox B1, Cox B5 and Cox B6 respectively. Meanwhile only 20.4% (37 cases) Cox B IgM positive cases could be detected in 181 non-myocarditis patients of same ages. The results showed that the positive rate of Cox B virus IgM antibody in clinically diagnosed myocarditis was significantly higher than that in control group (P <0.01).


CONCLUSIONS
The Cox B viruses may be the main agents causing viral myocarditis in Beijing cases. Mac ELISA is an easy, rapid, specific and sensitive method to detect Cox B virus IgM antibody for early and rapid diagnosis of myocarditis.
Zhu
H
H
Institute of Virology, Chinese Academy of Pre'oentire Medicine, Beijing 100052, China.
Zhang
L B
LB
D000914:Antibodies, Viral / Q000097:blood*; D029822:Enterovirus B, Human / Q000276:immunology*; D004769:Enterovirus Infections / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood*; D008297:Male; D009205:Myocarditis / Q000276:immunology / Q000821:virology*
D000914:Antibodies, Viral; D007075:Immunoglobulin M