[Isolation and identification of tyrosine kinase from the cytosolic fraction of bovine retinal rod outer segments].
It was shown that the cytosol fraction of bovine retinal rod outer segments contains three forms of tyrosine kinase. One of them was purified 171-fold to attain a specific activity of 1.6 nmol/min per mg protein. The isolated protein had a molecular weight of about 54,000 in SDS electrophoresis. It was shown that this protein is a tyrosine-specific protein kinase, capable of autophosphorylation at the tyrosine residues and restoration of kinase activity upon denaturation-renaturation.
Sholukh
A M
AM
a.sholukh@usa.net
Artamonov
I D
ID
Baranova
L A
LA
Lipkin
V M
VM
Volotovskiĭ
I D
ID
D000818:Animals; D002417:Cattle; D003600:Cytosol / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D010766:Phosphorylation; D011489:Protein Denaturation; D020673:Protein Renaturation; D011505:Protein-Tyrosine Kinases / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D012374:Rod Cell Outer Segment / Q000201:enzymology*
D011505:Protein-Tyrosine Kinases