title: Effect of ursodeoxycholate in University of Wisconsin solution on long term preservation of rat livers.
abstract: OBJECTIVE
  To find out whether the addition of ursodeoxycholate (UDC) to University of Wisconsin (UW) solution reduces injury during preservation of the liver.
  
  
  METHODS
  Controlled laboratory study.
  
  
  METHODS
  University hospital, Germany.
  
  
  METHODS
  Preserved livers from 12 rats.
  
  
  METHODS
  Livers were flushed with 10 ml of ice-cold (0-1 degrees C) UW or iso-osmolar UW containing 5 mmol/l UDC. After preservation for 30 hours at 0-1 degrees C and 1 hour at 22 degrees C the livers were reperfused for 4 hours. Aspartate amino-transaminase, alanine aminotransferase, glutamate lactate dehydrogenase, and white blood cell (WBC) and platelet counts were measured during reperfusion. Bile flow and total bile salt secretion rate were also measured.
  
  
  RESULTS
  Addition of UDC to UW reduced the release of liver enzymes, slightly increased platelet adherence, and reduced bile flow and bile salt secretion rate.
  
  
  CONCLUSIONS
  The membrane-stabilising effect of UDC may explain the reduction in enzyme release. However, liver function, as expressed by bile salt secretion rate and bile flow was adversely affected.
authors.affiliation: Department of General and Transplantation Surgery, University Hospital, Essen, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Hertl
authors.forename: N
authors.initials: N
authors.lastname: Clausnitzer
authors.forename: M C
authors.initials: MC
authors.lastname: Hertl
authors.forename: C E
authors.initials: CE
authors.lastname: Broelsch
mesh_terms: D000241:Adenosine / Q000737:chemistry*; D000493:Allopurinol / Q000737:chemistry*; D000818:Animals; D005978:Glutathione / Q000737:chemistry*; D066298:In Vitro Techniques; D007328:Insulin / Q000737:chemistry*; D008099:Liver; D008111:Liver Function Tests; D008297:Male; D023421:Models, Animal; D009926:Organ Preservation; D019188:Organ Preservation Solutions / Q000737:chemistry*; D011887:Raffinose / Q000737:chemistry*; D051381:Rats; D011917:Rats, Inbred Lew; D014580:Ursodeoxycholic Acid
chemical_list: D007328:Insulin; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; D014580:Ursodeoxycholic Acid; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose