[Construction and expression of recombinant adeno-associated HIV -1 virus].
OBJECTIVE
Plasmid of AAV vector with HIV-1 gag and gp 120 were constructed respectively.


METHODS
The recombinant AAV-HIV gag and gp120 viruses were obtained by co transfection of AAV-HIV plasmid and Adenovirus 5 in 293 cells.


RESULTS
The recombinant AAV-HIV virus expressed well and the titer of the recombinant viruses were between 10(4)-10(5).


CONCLUSIONS
The recombinant AAV-HIV virus can be used to infect the target cells for detection of cellular immunity and for vaccine studies.
Guan
Y
Y
Institute of Virology, Chinese Academy of Preventive Medicine, Beijing 100052, China.
Liu
H
H
Zhu
Y
Y
D000260:Adenoviruses, Human / Q000235:genetics*; D002478:Cells, Cultured; D015750:Genes, gag / Q000235:genetics; D005822:Genetic Vectors; D015699:HIV Envelope Protein gp120 / Q000235:genetics; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D010957:Plasmids; D011995:Recombination, Genetic
D015699:HIV Envelope Protein gp120