Lineage commitment and maturation induction in normal and leukemic preprogenitor cells.
Analysis of the critical cellular processes of self-generation, commitment, and maturation induction ideally requires the use of clonal cultures using cells with a capacity to undergo all three processes. Preprogenitor cells from normal mouse marrow are proving useful cells for such studies. Cells of a newly established cloned leukemic cell line, the GB2, are providing a useful analogous leukemic system because GB2 cells form stratified subpopulations of clonogenic cells able to be clonally analyzed in vitro in which self-renewal is demonstrable, but in which near-normal maturation can be induced by a wide range of hematopoietic regulators.
Metcalf
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D
Walter and Eliza Hall Institute of Medical Research, P.O. Royal Melbourne Hospital, 3050 Victoria, Australia. metcalf@wehi.edu.au
Laâbi
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Y
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects; D002454:Cell Differentiation / Q000187:drug effects; D019070:Cell Lineage; D002478:Cells, Cultured / Q000187:drug effects; D002999:Clone Cells / Q000187:drug effects; D003114:Colony-Forming Units Assay; D017077:Culture Media, Conditioned; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000187:drug effects*; D007377:Interleukin-3 / Q000494:pharmacology; D007942:Leukemia, Experimental / Q000473:pathology*; D008214:Lymphocytes / Q000166:cytology; D016173:Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D008565:Membrane Proteins / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D022423:Myeloid Cells / Q000166:cytology; D014411:Neoplastic Stem Cells / Q000166:cytology / Q000187:drug effects*; D019089:Stem Cell Factor / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects; D014410:Tumor Stem Cell Assay; D014916:Whole-Body Irradiation
D017077:Culture Media, Conditioned; D007377:Interleukin-3; D008565:Membrane Proteins; D019089:Stem Cell Factor; C084881:flt3 ligand protein; D016173:Macrophage Colony-Stimulating Factor