[Synthesis of oligodeoxyribonucleotides containing oleylamine moieties].
A method for directional introduction of oleylamine residues to any position of oligodeoxyribonucleotides during their automated synthesis was developed. The presence of oleylamine residues in 3'- or 5'-terminal nucleotides was shown to have no effect on the thermodynamic stability of DNA duplexes formed by such oligonucleotides and the complementary sequences. The rate of the snake venom phosphodiesterase hydrolysis of oligonucleotides containing oleylamine residues in the 3'-terminal units was shown to be markedly lower than that of natural oligonucleotides.
Andreev
S Iu
SIu
Department of Chemistry and Belozersky, Institute of Physico-Chemical Biology, Moscow State University, Vorob'evy gory, Moscow, 119899 Russia.
Antsypovich
S I
SI
Volkov
E M
EM
Romanova
E A
EA
Hianik
T
T
Oretskaia
T S
TS
D000588:Amines / Q000138:chemical synthesis* / Q000737:chemistry; D000818:Animals; D004247:DNA / Q000737:chemistry; D006868:Hydrolysis; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D010727:Phosphoric Diester Hydrolases; D012910:Snake Venoms
D000588:Amines; D009838:Oligodeoxyribonucleotides; D012910:Snake Venoms; D004247:DNA; D010727:Phosphoric Diester Hydrolases; C008703:oleylamine