How Drosophila males make eggs: it is elemental.
Oogenesis in Drosophila requires a significant amount of phosphorus. Oocytes mature in follicles, each of which contains 15 highly polyploid, transcriptionally active chromosomes. We show that the demand for phosphorus is met in part from the male's ejaculate following mating. Females incorporate phosphorus-32 from radiolabelled males into their ovaries, specifically into their nucleic acids. Male-derived phosphorus is also present in significant amounts in mature oocytes. The mechanism by which phosphorus uptake from the female reproductive tract occurs must differ from that previously reported for radiolabelled carbon and hydrogen derived from ejaculatory proteins, as phosphorus uptake is observed in species not showing female incorporation of radiolabel derived from ejaculate proteins.
Markow
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Department of Ecology and Evolutionary Biology, University of Arizona, Tucson, AZ 85721, USA. markow@arl.arizona.edu
Coppola
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Watts
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D000818:Animals; D003307:Copulation; D004247:DNA / Q000032:analysis; D004330:Drosophila / Q000502:physiology*; D005260:Female; D008297:Male; D009696:Nucleic Acids / Q000378:metabolism; D009865:Oocytes / Q000502:physiology; D009866:Oogenesis / Q000502:physiology*; D010053:Ovary / Q000502:physiology; D010758:Phosphorus / Q000502:physiology*; D010761:Phosphorus Radioisotopes; D011003:Ploidies; D012313:RNA / Q000032:analysis
D009696:Nucleic Acids; D010761:Phosphorus Radioisotopes; D010758:Phosphorus; D012313:RNA; D004247:DNA