Retinoic acid induction of sialyltransferase activity in neuroblastoma cells of differing sialylation potentials.
In order to determine how glycosylation changes associated with cellular differentiation may be influenced by the basal cellular sialylation potential, the effect of retinoic acid (RA)-induced differentiation was investigated in neuroblastoma cells expressing differing levels (and activities) of the alpha2,6(N) sialyltransferase (ST6N) enzyme. The increase in ST activity was proportional to the basal cellular sialylation potentials with the high activity clones showing the greatest increase. This was paralleled by an up-regulation of the level of overall sialoglycoprotein glycosylation level. An increase in the levels of the polysialic acid (PSA) epitope was associated with a parallel increase in the levels of the neural cell adhesion molecule (NCAM) protein backbone although there was no overall change in the PSA:NCAM ratio following RA treatment.
Georgopoulou
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N
Dept. of Psychiatry, University of Dundee, Ninewells Hospital Medical School, Dundee DD1 9SY, Scotland, UK.
Breen
K C
KC
D000818:Animals; D002454:Cell Differentiation; D004790:Enzyme Induction; D000939:Epitopes / Q000096:biosynthesis; D019006:Neural Cell Adhesion Molecules / Q000096:biosynthesis* / Q000276:immunology; D009447:Neuroblastoma; D051381:Rats; D011994:Recombinant Proteins / Q000096:biosynthesis; D012794:Sialic Acids / Q000096:biosynthesis* / Q000276:immunology; D012799:Sialyltransferases / Q000096:biosynthesis* / Q000235:genetics; D014212:Tretinoin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase
D000939:Epitopes; D019006:Neural Cell Adhesion Molecules; D011994:Recombinant Proteins; D012794:Sialic Acids; C021319:polysialic acid; D014212:Tretinoin; D012799:Sialyltransferases; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase