The extraction of polymeric collagen from biopsies of human skin.
1. Medium sized biopsies (100 mm2) of human skin from 14 subjects yielded sufficient polymeric collagen for depolymerisation and ultrastructural investigations. 2. The yields obtained from one skin specimen by the alpha-amylase, EDTA and lyotropic relaxation (water) methods of extracting polymeric collagen are similar. 3. The responses to depolymerisation treatments of the three polymeric collagen samples extracted by each of the three methods from one skin specimen are cross-correlated. There are however electron microscopical differences between the three polymeric collagen samples. 4. The results show that it feasible to study the polymeric collagen of normal and diseased human skin from medium sized biopsies.
Francis
M J
MJ
Nuffield Department of Orthopaedic Surgery, University of Oxford, Nuffield Orthopaedic Centre, Oxford OX3 7LD.
Macmillan
D C
DC
D000328:Adult; D000368:Aged; D001706:Biopsy; D003094:Collagen / Q000737:chemistry / Q000302:isolation & purification*; D006801:Humans; D007202:Indicators and Reagents; D008875:Middle Aged; D011402:Pronase; D011489:Protein Denaturation; D012867:Skin / Q000737:chemistry* / Q000166:cytology; D000516:alpha-Amylases
D007202:Indicators and Reagents; D003094:Collagen; D000516:alpha-Amylases; D011402:Pronase