Orexin (hypocretin) gene expression in rat ependymal cells.
The expression of prepro-orexin (PPO) mRNA in the rat brain was investigated by in situ hybridization histochemistry. In the lateral and posterior hypothalamic areas, which are considered to produce exclusively PPO mRNA, we found high levels of PPO mRNA expressions. We also localized PPO mRNA hybridization signals at lower levels around the lateral ventricles, the third and fourth ventricle. Cellular analysis by emulsion autoradiography revealed the expression of PPO mRNA in the ependymal cell layer. Our results demonstrate that beside the lateral and posterior hypothalamus PPO mRNA is expressed in ependymal cells.
Kummer
M
M
Institute of Experimental and Clinical Pharmacology and Toxicology, University Clinic Lübeck, Ratzeburger Allee 160, D-23538 Lübeck, Germany.
Neidert
S J
SJ
Jöhren
O
O
Dominiak
P
P
D000818:Animals; D004805:Ependyma / Q000166:cytology* / Q000378:metabolism*; D020546:Fourth Ventricle / Q000166:cytology / Q000378:metabolism; D005786:Gene Expression Regulation / Q000502:physiology*; D007026:Hypothalamic Area, Lateral / Q000166:cytology / Q000378:metabolism; D047908:Intracellular Signaling Peptides and Proteins; D020547:Lateral Ventricles / Q000166:cytology / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000096:biosynthesis* / Q000235:genetics*; D000068797:Orexins; D011498:Protein Precursors / Q000096:biosynthesis* / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D020542:Third Ventricle / Q000166:cytology / Q000378:metabolism
D047908:Intracellular Signaling Peptides and Proteins; D009479:Neuropeptides; D000068797:Orexins; D011498:Protein Precursors; D012333:RNA, Messenger