title: Type I IFN modulates the immune response induced by DNA vaccination to pseudorabies virus glycoprotein C.
abstract: DNA vaccines have the capacity to induce strong Th1-biased immune responses that are of major importance to providing protection against intracellular pathogens. In the present study we have focused on the role played by type I IFN in immune responses induced after DNA vaccination. Mice lacking the IFNAR1 chain of the type I IFN receptor (IFNAR K/O mice) were immunized with a plasmid encoding glycoprotein C of pseudorabies virus (PRV-gC). After DNA vaccination, wild-type (WT) mice showed features characteristic of Th1 immune responses, such as high IgG2a:IgG1 anti-PRV Ab ratio and antigen-specific IFN-gamma production by spleen cells. In contrast, IFNAR K/O mice showed a significantly lower IgG2a:IgG1 Ab ratio and IFN-gamma production. In addition, the percentage of CD8(+) and B lymph-node cells expressing CD69 after PRV-gC DNA vaccination was lower in IFNAR K/O than in WT mice. These results support a major role played by type I IFN in shaping Th1 immune responses after DNA vaccination. Codelivery of plasmids encoding IL-12 and IL-18 along with the plasmid encoding PRV-gC restored Th1 responses in IFNAR K/O mice.
authors.affiliation: Institut National de la Recherche Agronomique (INRA), Unité de Virologie et d'Immunologie Moléculaires, Jouy-en-Josas, 78350, France.
authors.forename: D
authors.initials: D
authors.lastname: Tudor
authors.forename: S
authors.initials: S
authors.lastname: Riffault
authors.forename: C
authors.initials: C
authors.lastname: Carrat
authors.forename: F
authors.initials: F
authors.lastname: Lefèvre
authors.forename: M
authors.initials: M
authors.lastname: Bernoin
authors.forename: B
authors.initials: B
authors.lastname: Charley
mesh_terms: D000818:Animals; D011558:Herpesvirus 1, Suid / Q000276:immunology*; D007109:Immunity; D007372:Interferons / Q000276:immunology*; D051379:Mice; D011557:Pseudorabies / Q000276:immunology* / Q000517:prevention & control; D022421:Pseudorabies Vaccines / Q000276:immunology*; D017471:Receptors, Interferon / Q000276:immunology; D014611:Vaccination; D014759:Viral Envelope Proteins / Q000276:immunology
chemical_list: D022421:Pseudorabies Vaccines; D017471:Receptors, Interferon; D014759:Viral Envelope Proteins; C042966:pseudorabies virus glycoproteins; D007372:Interferons