title: Self-association and membrane-binding behavior of melittins containing trifluoroleucine.
abstract: We have investigated the effect of trifluoroleucine substitution on the membrane-binding and tetramerization behavior of melittin. Analogues were synthesized in which Leu 9, Leu 13, and all four intrinsic leucine residues of melittin were replaced by 5,5,5-trifluoroleucine. Both the mono- and tetra-substituted melittins were found to exhibit stronger self-association and enhanced affinity for lipid bilayer membranes, compared to the wild-type peptide. The extent of the observed effects depends on the site of introduction of trifluoroleucine and, in the case of substitution at position 13, on the stereochemistry of the trifluoroleucine side chain. Analysis of the membrane association isotherms is consistent with aggregation of fluorinated melittins within the lipid bilayer. These results suggest that fluorocarbon-hydrocarbon separation, in addition to an increase in hydrophobic character, contributes to enhanced membrane binding.
authors.affiliation: Contribution from the Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125, USA.
authors.forename: A
authors.initials: A
authors.lastname: Niemz
authors.forename: D A
authors.initials: DA
authors.lastname: Tirrell
mesh_terms: D000595:Amino Acid Sequence; D001704:Biopolymers; D018360:Crystallography, X-Ray; D007930:Leucine / Q000031:analogs & derivatives* / Q000737:chemistry*; D008051:Lipid Bilayers; D008555:Melitten / Q000737:chemistry / Q000378:metabolism*; D008567:Membranes, Artificial; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D011487:Protein Conformation; D013237:Stereoisomerism
chemical_list: D001704:Biopolymers; D008051:Lipid Bilayers; D008567:Membranes, Artificial; D010743:Phospholipids; D008555:Melitten; C035177:5',5',5'-trifluoroleucine; D007930:Leucine