title: Increased expression of P-cadherin mRNA in the mouse peritoneum after carbon dioxide insufflation.
abstract: BACKGROUND
  Although systemic responses to carbon dioxide (CO(2)) pneumoperitoneum have been studied, there have been few reports of local responses within the peritoneum. We investigated the expression of mRNA for adhesion molecules involved in cell-cell interactions, including ICAM-1, VCAM-1, CD44, E-cadherin, P-cadherin, and N-cadherin, after the induction of a CO(2) pneumoperitoneum in mice.
  
  
  METHODS
  Mice were treated with CO(2) pneumoperitoneum (4-6 mmHg for 30 min) and then killed after 24 h, 48 h, and 72 h. The peritoneum of the abdominal wall was resected, and total RNA was extracted by the acid guanidinium thiocyanate-phenol-chloroform extraction procedure, cDNA were synthesized by reverse transcription. Expression of the mRNA for each gene was normalized to that of b-actin for semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR).
  
  
  RESULTS
  The expression of P-cadherin mRNA was significantly increased at 48 h (p = 0.007) and returned to the control level by 72 h after CO(2) pneumoperitoneum. The expression of CD44 increased gradually, reaching a peak at 48 h and returning to the control value by 72 h after CO(2) pneumoperitoneum. Expression of ICAM-1 mRNA was not changed significantly after the application of CO(2).
  
  
  CONCLUSIONS
  The expression of P-cadherin mRNA in the peritoneum can be induced to repair injuries to mesothelial cells caused by CO(2) pneumoperitoneum.
authors.affiliation: Department of Surgery I, Oita Medical University, 1-1 Idaigaoka, Hasama-machi, Oita 879-5593, Japan. ktahara@oita-med.ac.jp
authors.forename: K
authors.initials: K
authors.lastname: Tahara
authors.forename: K
authors.initials: K
authors.lastname: Fujii
authors.forename: K
authors.initials: K
authors.lastname: Yamaguchi
authors.forename: T
authors.initials: T
authors.lastname: Suematsu
authors.forename: N
authors.initials: N
authors.lastname: Shiraishi
authors.forename: S
authors.initials: S
authors.lastname: Kitano
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D015820:Cadherins / Q000032:analysis / Q000378:metabolism*; D002245:Carbon Dioxide / Q000008:administration & dosage / Q000494:pharmacology*; D015815:Cell Adhesion Molecules / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D007327:Insufflation; D051379:Mice; D023421:Models, Animal; D010537:Peritoneum / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D011028:Pneumoperitoneum, Artificial / Q000379:methods*; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000706:statistics & numerical data; D019010:Vascular Cell Adhesion Molecule-1 / Q000378:metabolism
chemical_list: D000199:Actins; D015820:Cadherins; D015815:Cell Adhesion Molecules; D012333:RNA, Messenger; D019010:Vascular Cell Adhesion Molecule-1; D002245:Carbon Dioxide