title: Solid-phase extraction and simplified high-performance liquid chromatographic determination of 10-deacetylbaccatin III and related taxoids in yew species.
abstract: Solid-phase extraction was accomplished with specially prepared cartridges filled with silanised silica gel (RP-2) for the purpose of 10-deacetylbaccatin III (10-DAB III) and related taxoids extracts purification obtained from different yew materials. In the first method, the analysed taxoids eluted in 75% methanol, but in the second method, the preliminary elution with 30% methanol was made. DAB III and its six derivatives were separated from co-extractives in merely acetonitrile-water gradient mode during 25 min on Waters Symmetry C-18 column with photodiode array (PDA) detection. The total recoveries for 10-DAB III and paclitaxel in the first SPE method (all compounds were applied in amounts of 80 microg) were about 98 and 94%, respectively. Almost 100% recoveries for paclitaxel and baseline separation of 10-DAB III and co-extracted compounds were obtained when preliminary elution with 30% methanol was performed. This method can be applied as a routine, inexpensive and uncomplicated procedure for 10-DAB III and related taxoids determination in yew material.
authors.affiliation: Department of Pharmacognosy, Medical University, Peowiakow 12 St., 20007 Lublin, Poland.
authors.forename: T
authors.initials: T
authors.lastname: Mroczek
authors.forename: K
authors.initials: K
authors.lastname: Glowniak
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000032:analysis* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D017239:Paclitaxel / Q000032:analysis* / Q000302:isolation & purification; D010936:Plant Extracts / Q000032:analysis*; D010946:Plants, Medicinal; D058428:Silica Gel; D012822:Silicon Dioxide; D043823:Taxoids; D020946:Taxus; D013729:Terpenes / Q000032:analysis* / Q000302:isolation & purification; D014315:Triterpenes / Q000032:analysis* / Q000302:isolation & purification
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts; D043823:Taxoids; D013729:Terpenes; D014315:Triterpenes; C042213:10-deacetylbaccatine III; D058428:Silica Gel; D012822:Silicon Dioxide; D017239:Paclitaxel