title: Differential expression for tissue-specific isozymes in three species of Hypostomus Lacépède, 1803 (Teleostei: Loricariidae).
abstract: The expression patterns of 14 enzymatic systems in skeletal muscle, liver and heart tissues of three species of Hypostomus from the Iguaçu River basin (Brazil) were investigated. Although the patterns were similar for the three species, different tissues showed differential expressions, and the data showed that differential tissue expressions of isoperoxidases may be due to preferential combination or association of polypeptide subunits. The detected patterns for SOD isozymes showed that the quaternary structures of these enzymes were in disagreement with the subunit number reported for the majority of other vertebrate groups. Tissue-specific restriction on heterotetramer formation also were described in LDH and MDHP isozymes. Thus, these tissue-specific gene expression character in the species of Hypostomus have the greatest potential to be recognized and applied in systematic studies among species of Hypostomus.
authors.affiliation: Nupélia (Research Center on Limnology, Ichthyology and Aquaculture), Brazil
authors.forename: C H.
authors.initials: CH
authors.lastname: Zawadzki
authors.forename: M F.P.S.
authors.initials: MF
authors.lastname: Machado
authors.forename: E
authors.initials: E
authors.lastname: Renesto