title: Expression of decorin and biglycan in rat gastric tissue: effects of ulceration and basic fibroblast growth factor.
abstract: BACKGROUND
  The small chondroitin/dermatan sulphate proteoglycans decorin and biglycan participate in organizing the network of collagen fibrils and interact with non-collagenous matrix proteins. In addition, via interactions with cytokines they are directly or indirectly involved in signalling, growth and cell differentiation. We aimed to analyse their expression in normal gastric tissue and during gastric ulcer healing.
  
  
  METHODS
  Proteoglycan expression was studied by immunohistochemistry and in situ hybridization in acetic acid-induced gastric ulcers in rat during early phases and during chronic ulceration. The effects of treatment with an acid stable mutein of FGF-2 (bFGF) were also studied.
  
  
  RESULTS
  In normal gastric tissue, both proteoglycans were most strongly expressed in the submucosal layer. However, some epithelial cells were positive for biglycan and, surprisingly, also for decorin. In the early phase after ulcer induction exclusively decorin became induced in the muscularis mucosae, while biglycan became detectable in this layer only after 2 weeks. There was no up-regulation of either proteoglycan in other layers, nor could an effect of FGF-2 treatment be seen.
  
  
  CONCLUSIONS
  The expression of decorin could be observed for the first time in epithelial cells. Decorin, but not biglycan, appears as an early phase reactant in the muscularis mucosae in accordance with its putative role during angiogenesis and the prevention of apoptosis.
authors.affiliation: Dept. of Medicine B, University of Münster, Germany. pohlet@uni-muenster.de
authors.forename: T
authors.initials: T
authors.lastname: Pohle
authors.forename: M
authors.initials: M
authors.lastname: Altenburger
authors.forename: M
authors.initials: M
authors.lastname: Shahin
authors.forename: J W
authors.initials: JW
authors.lastname: Konturek
authors.forename: H
authors.initials: H
authors.lastname: Kresse
authors.forename: W
authors.initials: W
authors.lastname: Domschke
mesh_terms: D019342:Acetic Acid; D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology; D058578:Biglycan; D058575:Decorin; D004195:Disease Models, Animal; D004353:Drug Evaluation, Preclinical; D016326:Extracellular Matrix Proteins; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D005753:Gastric Mucosa / Q000033:anatomy & histology* / Q000187:drug effects* / Q000502:physiology; D015870:Gene Expression / Q000187:drug effects* / Q000502:physiology*; D007150:Immunohistochemistry; D008297:Male; D018919:Neovascularization, Physiologic / Q000187:drug effects / Q000502:physiology; D011509:Proteoglycans / Q000187:drug effects* / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D013276:Stomach Ulcer / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology*; D013997:Time Factors; D015854:Up-Regulation; D014945:Wound Healing / Q000187:drug effects* / Q000502:physiology*
chemical_list: C546886:Bgn protein, rat; D058578:Biglycan; C546880:Dcn protein, rat; D058575:Decorin; D016326:Extracellular Matrix Proteins; D011509:Proteoglycans; D016222:Fibroblast Growth Factor 2; D019342:Acetic Acid