Purification, crystallization and identification by X-ray analysis of a prostate kallikrein from horse seminal plasma.
The purification, crystallization and identification by X-ray diffraction analysis of a horse kallikrein is reported. The protein was purified from horse seminal plasma. Crystals belong to space group C2 and the structure was solved by the MIRAS method, with two heavy-atom derivatives of mercury and platinum. X-ray diffraction data to 1.42 A resolution were collected at the ESRF synchrotron-radiation source.
Carvalho
A L
AL
Departamento de Química, Centro de Química Fina e Biotecnologia, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516 Monte de Caparica, Portugal.
Dias
J M
JM
Sanz
L
L
Romero
A
A
Calvete
J J
JJ
Romão
M J
MJ
D000595:Amino Acid Sequence; D000818:Animals; D003460:Crystallization; D018360:Crystallography, X-Ray; D006736:Horses; D007610:Kallikreins / Q000737:chemistry* / Q000302:isolation & purification; D008297:Male; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011467:Prostate / Q000737:chemistry*; D011487:Protein Conformation; D012661:Semen / Q000737:chemistry*; D017386:Sequence Homology, Amino Acid
D007610:Kallikreins