Astrovirus detection in wastewater samples.
Procedures for the detection of astroviruses in wastewater samples have been developed and evaluated. Following these methodologies, we investigated the occurrence of astroviruses in wastewater samples from three different sewage treatments plants located in Southern France and two in the Barcelona area. Some positive samples were genotyped by analysis of a fragment of the ORF1a by restriction fragment length polymorphism (RFLP) with endonuclease DdeI. The amplimers generated contain several sites for the DdeI restriction endonuclease, being the number and location of sites different between strains.
Pintó
R M
RM
Dept of Microbiology, University of Barcelona, 08028 Barcelona, Spain. pinto@porthos.bio.ub.es
Villena
C
C
Le Guyader
F
F
Guix
S
S
Caballero
S
S
Pommepuy
M
M
Bosch
A
A
D004279:DNA, Viral / Q000032:analysis; D004784:Environmental Monitoring / Q000379:methods; D006801:Humans; D001257:Mamastrovirus / Q000235:genetics; D012150:Polymorphism, Restriction Fragment Length; D011634:Public Health; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012722:Sewage / Q000821:virology; D014881:Water Supply
D004279:DNA, Viral; D012722:Sewage