title: Changes of activity inducing chromosomal aberrations and transformations of chlorinated humic acid.
abstract: The change of the toxicity of chlorinated water after chlorine injection was examined. For the measurement of toxicity, chromosomal aberration test and transforming test were carried out as indexes to initiating activity and to promoting activity in the carcinogenesis process, respectively. Activity inducing chromosomal aberrations of chlorinated humic acid gradually decreased with time after chlorination. In contrast, activity inducing transformations measured by the two-stage assay gradually increased. Thus, the toxicity that decreases or increases is present in chlorinated water. Furthermore, activity inducing transformations measured by the non-two-stage assay gradually decreased. This direction of change was reverse to that of activity inducing transformations by the two-stage assay and consistent with that of activity inducing chromosomal aberrations. It is speculated that the main reason of decreasing activity inducing transformations by the non-two-stage assay is because initiating activity detected as activity inducing chromosomal aberrations in chlorinated water decreases drastically. Directions of changes of total organic halogen and carbonyl group were qualitatively consistent with that of activity inducing chromosomal aberrations. Directions of changes of chloroform and dichloroacetic acid were qualitatively consistent with that of activity inducing transformations by the two-stage assay. Findings of this study suggest that further research is necessary to compare carcinogenicity of tap water near water purification plant and distant tap water.
authors.affiliation: Department of Environmental Engineering, Kyoto University, Japan. k53041@sakura.kudpc.kyoto-u.ac.jp
authors.forename: S
authors.initials: S
authors.lastname: Itoh
authors.forename: D
authors.initials: D
authors.lastname: Ikeda
authors.forename: Y
authors.initials: Y
authors.lastname: Toba
authors.forename: H
authors.initials: H
authors.lastname: Sumitomo
mesh_terms: D000818:Animals; D001681:Biological Assay; D015197:Carcinogenicity Tests; D002274:Carcinogens, Environmental / Q000633:toxicity; D002460:Cell Line; D002713:Chlorine / Q000633:toxicity*; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000187:drug effects; D006224:Cricetinae; D004202:Disinfectants / Q000633:toxicity; D005347:Fibroblasts / Q000187:drug effects; D006812:Humic Substances / Q000633:toxicity*; D006843:Hydrocarbons, Chlorinated / Q000633:toxicity*; D006863:Hydrogen-Ion Concentration; D008168:Lung / Q000166:cytology / Q000187:drug effects; D051379:Mice; D014881:Water Supply / Q000032:analysis*
chemical_list: D002274:Carcinogens, Environmental; D004202:Disinfectants; D006812:Humic Substances; D006843:Hydrocarbons, Chlorinated; D002713:Chlorine