title: Disaccharide modulation of the mitochondrial membrane fluidity changes induced by the membrane potential.
abstract: The influence of the medium composition on the dynamic properties of mitochondrial membranes on depolarization was studied by following the fluorescence anisotropy changes of mitochondria-bound 1,6-diphenyl-1,3,5-hexatriene (DPH) and hematoporphyrin (HP) as reporters, respectively, of lipid and protein regions. On collapse of the potential, the membrane fluidity increased in NaCl-, KCl-, and monosaccharide-based media and decreased in disaccharides. Infrared spectroscopy experiments suggested that disaccharides likely change water's structure and association on the membrane surface. These results indicate that disaccharides induce membrane perturbation, which may interfere in the study of structure-function correlation in biological membranes.
authors.affiliation: CNR, Centro Metalloproteine, Dipartimento di Biologia, Università di Padova, Italy. rchieppi@civ.bio.unipd.it
authors.forename: F
authors.initials: F
authors.lastname: Ricchelli
authors.forename: M
authors.initials: M
authors.lastname: Camerin
authors.forename: C
authors.initials: C
authors.lastname: Beghetto
authors.forename: M
authors.initials: M
authors.lastname: Crisma
authors.forename: V
authors.initials: V
authors.lastname: Moretto
authors.forename: S
authors.initials: S
authors.lastname: Gobbo
authors.forename: B
authors.initials: B
authors.lastname: Salvato
authors.forename: C
authors.initials: C
authors.lastname: Salet
authors.forename: G
authors.initials: G
authors.lastname: Moreno
mesh_terms: D000818:Animals; D004187:Disaccharides / Q000494:pharmacology*; D007425:Intracellular Membranes / Q000187:drug effects* / Q000502:physiology; D008560:Membrane Fluidity / Q000187:drug effects*; D008564:Membrane Potentials / Q000502:physiology*; D051381:Rats; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D004187:Disaccharides