title: Cytotoxicity of root canal filling materials to three different human cell lines.
abstract: The aim of this study was to investigate the biological compatibility of five root canal sealers (Sealapex, Endion, Super-EBA, Ketac-Endo, and AH Plus) and regular and calcium hydroxide-based gutta-percha in three different human cell lines. Cultures without root canal sealers were used as controls. Cell growth, cell morphology, cell viability, protein content of the cells, and prostaglandin E2 (PGE2) release were used as parameters to determine the cytotoxicity of the materials. The protein content of the three cell lines--nasal fibroblasts, gingival fibroblasts, and epithelial tumor cells--was significantly reduced (p < or = 0.001) by all materials tested. Determinations of PGE2 release showed significant material specific differences. No significant increased PGE2 release values were found with Sealapex, Endion, and Super-EBA. On the contrary significantly increased PGE2 levels were measured with Ketac Endo, AH Plus, regular and calcium hydroxide-based gutta-percha points (p < or = 0.001).
authors.affiliation: Department of Operative Dentistry and Periodontology, Johannes Gutenberg-University, Mainz, Germany.
authors.forename: B
authors.initials: B
authors.lastname: Willershausen
authors.forename: B B
authors.initials: BB
authors.lastname: Marroquín
authors.forename: D
authors.initials: D
authors.lastname: Schäfer
authors.forename: R
authors.initials: R
authors.lastname: Schulze
mesh_terms: D001672:Biocompatible Materials / Q000633:toxicity*; D015415:Biomarkers / Q000032:analysis; D002126:Calcium Hydroxide / Q000633:toxicity; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D017220:Dentin-Bonding Agents / Q000633:toxicity; D015232:Dinoprostone / Q000032:analysis / Q000378:metabolism; D004853:Epoxy Resins / Q000633:toxicity; D005347:Fibroblasts / Q000187:drug effects*; D005881:Gingiva / Q000166:cytology / Q000187:drug effects; D005899:Glass Ionomer Cements / Q000633:toxicity; D006171:Gutta-Percha / Q000633:toxicity; D006801:Humans; D009297:Nasal Mucosa / Q000166:cytology / Q000187:drug effects; D009375:Neoplasms, Glandular and Epithelial / Q000473:pathology; D011506:Proteins / Q000187:drug effects; D012387:Root Canal Filling Materials / Q000633:toxicity*; D012459:Salicylates / Q000633:toxicity; D013223:Statistics as Topic; D014407:Tumor Cells, Cultured
chemical_list: D001672:Biocompatible Materials; D015415:Biomarkers; D017220:Dentin-Bonding Agents; C098488:Endion; D004853:Epoxy Resins; D005899:Glass Ionomer Cements; C074934:Ketac Endo; D011506:Proteins; D012387:Root Canal Filling Materials; D012459:Salicylates; C534916:epoxy resin-based root canal sealer; C055601:Sealapex; C084074:Super EBA; D006171:Gutta-Percha; D015232:Dinoprostone; D002126:Calcium Hydroxide