title: Annotated draft genomic sequence from a Streptococcus pneumoniae type 19F clinical isolate.
abstract: The public availability of numerous microbial genomes is enabling the analysis of bacterial biology in great detail and with an unprecedented, organism-wide and taxon-wide, broad scope. Streptococcus pneumoniae is one of the most important bacterial pathogens throughout the world. We present here sequences and functional annotations for 2.1-Mbp of pneumococcal DNA, covering more than 90% of the total estimated size of the genome. The sequenced strain is a clinical isolate resistant to macrolides and tetracycline. It carries a type 19F capsular locus, but multilocus sequence typing for several conserved genetic loci suggests that the strain sequenced belongs to a pneumococcal lineage that most often expresses a serotype 15 capsular polysaccharide. A total of 2,046 putative open reading frames (ORFs) longer than 100 amino acids were identified (average of 1,009 bp per ORF), including all described two-component systems and aminoacyl tRNA synthetases. Comparisons to other complete, or nearly complete, bacterial genomes were made and are presented in a graphical form for all the predicted proteins.
authors.affiliation: Research Department, GlaxoSmithKline S.A., Tres Cantos, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Dopazo
authors.forename: A
authors.initials: A
authors.lastname: Mendoza
authors.forename: J
authors.initials: J
authors.lastname: Herrero
authors.forename: F
authors.initials: F
authors.lastname: Caldara
authors.forename: Y
authors.initials: Y
authors.lastname: Humbert
authors.forename: L
authors.initials: L
authors.lastname: Friedli
authors.forename: M
authors.initials: M
authors.lastname: Guerrier
authors.forename: E
authors.initials: E
authors.lastname: Grand-Schenk
authors.forename: C
authors.initials: C
authors.lastname: Gandin
authors.forename: M
authors.initials: M
authors.lastname: de Francesco
authors.forename: A
authors.initials: A
authors.lastname: Polissi
authors.forename: G
authors.initials: G
authors.lastname: Buell
authors.forename: G
authors.initials: G
authors.lastname: Feger
authors.forename: E
authors.initials: E
authors.lastname: García
authors.forename: M
authors.initials: M
authors.lastname: Peitsch
authors.forename: J F
authors.initials: JF
authors.lastname: García-Bustos
mesh_terms: D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics; D016680:Genome, Bacterial; D008969:Molecular Sequence Data; D011008:Pneumococcal Infections / Q000382:microbiology*; D011387:Prokaryotic Cells; D012343:RNA, Transfer / Q000378:metabolism; D013296:Streptococcus pneumoniae / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; D012343:RNA, Transfer