title: [Construction and expression of recombinant adeno-associated HIV -1 virus].
abstract: OBJECTIVE
  Plasmid of AAV vector with HIV-1 gag and gp 120 were constructed respectively.
  
  
  METHODS
  The recombinant AAV-HIV gag and gp120 viruses were obtained by co transfection of AAV-HIV plasmid and Adenovirus 5 in 293 cells.
  
  
  RESULTS
  The recombinant AAV-HIV virus expressed well and the titer of the recombinant viruses were between 10(4)-10(5).
  
  
  CONCLUSIONS
  The recombinant AAV-HIV virus can be used to infect the target cells for detection of cellular immunity and for vaccine studies.
authors.affiliation: Institute of Virology, Chinese Academy of Preventive Medicine, Beijing 100052, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Guan
authors.forename: H
authors.initials: H
authors.lastname: Liu
authors.forename: Y
authors.initials: Y
authors.lastname: Zhu
mesh_terms: D000260:Adenoviruses, Human / Q000235:genetics*; D002478:Cells, Cultured; D015750:Genes, gag / Q000235:genetics; D005822:Genetic Vectors; D015699:HIV Envelope Protein gp120 / Q000235:genetics; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D010957:Plasmids; D011995:Recombination, Genetic
chemical_list: D015699:HIV Envelope Protein gp120