Long-term preservation of strains of Burkholderia cepacia, Pseudomonas spp. and Stenotrophomonas maltophilia isolated from patients with cystic fibrosis.
Long-term preservation methods are important in the maintenance of bacteria for downstream research applications. Most clinical laboratories have only limited resources for archiving isolates and therefore require cost-effective and simple methods. An effective and cheap storage method using debrinated blood and maintenance at -80 degrees C is described.
Moore
J E
JE
Northern Ireland Public Health Laboratory, Department of Bacteriology, Belfast City Hospital, UK. jemoore@niphl.dnet.co.uk
Shaw
A B
AB
Stanley
T
T
Crowe
M J
MJ
Elborn
J S
JS
D001431:Bacteriological Techniques; D016956:Burkholderia cepacia / Q000502:physiology; D015925:Cryopreservation; D003550:Cystic Fibrosis / Q000382:microbiology*; D006801:Humans; D011549:Pseudomonas / Q000502:physiology; D013048:Specimen Handling; D020615:Stenotrophomonas maltophilia; D013997:Time Factors