title: Impact of plasmapheresis on platelet hemostatic capacity in healthy voluntary blood donors detected by the platelet function analyzer PFA-100.
abstract: Previous flow cytometry studies showed that platelet activation may occur during cytapheresis, cardiopulmonary bypass and hemodialysis. The aim of this pilot study was to determine if the impact of plasmapheresis leads to alterations in platelet hemostatic capacity. Plasmapheresis was carried out in 30 volunteers using an Autopherese C (Baxter) and an MCS3p (Haemonetics). Blood samples were collected and analyzed immediately before and after plasmapheresis. As a result of the plasmapheresis on the Autopherese C, the mean closure time (CT) for collagen/epinephrine (Col/Epi) increased from 118.2 +/- 25.1 to 149.9 +/- 35.0 s and from 88.7 +/- 16.9 to 98.5 +/- 26.3 s for collagen/ADP (Col/ADP), respectively. Seven subjects showed impaired CTs after plasmapheresis for Col/Epi. For Col/ADP, five subjects showed impaired CTs after apheresis. Statistical analysis (McNemar test) showed a significant difference before and after apheresis for Col/Epi (P = 0.024) but not for Col/ADP (P = 0.088). Similar results were shown for plasmapheresis carried out with the MCS3p. These findings show that plasmapheresis can cause an impairment of platelet function in healthy volunteers as measured by the PFA-100, an effect of so far unknown clinical significance.
authors.affiliation: Institute of Clinical Pharmacology, Faculty of Clinical Medicine Mannheim, University of Heidelberg, Heidelberg, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Feuring
authors.forename: A
authors.initials: A
authors.lastname: Gutfleisch
authors.forename: A
authors.initials: A
authors.lastname: Ganschow
authors.forename: E
authors.initials: E
authors.lastname: Richter
authors.forename: H
authors.initials: H
authors.lastname: Eichler
authors.forename: C E
authors.initials: CE
authors.lastname: Dempfle
authors.forename: H C
authors.initials: HC
authors.lastname: Tillmann
authors.forename: A
authors.initials: A
authors.lastname: Schultz
authors.forename: M
authors.initials: M
authors.lastname: Wehling
mesh_terms: D000328:Adult; D001782:Blood Donors; D001792:Blood Platelets / Q000502:physiology*; D006487:Hemostasis; D006801:Humans; D010956:Plasmapheresis / Q000009:adverse effects; D015539:Platelet Activation