Identification of fibroblast growth factor-5 as an overexpressed antigen in multiple human adenocarcinomas.
Methodology for identifying tumor-associated antigens recognized by T cells has been successfully used to clone antigens from melanoma cells. Similar efforts for nonmelanoma tumors have had limited success with few antigens identified. To identify potentially relevant tumor-associated antigens expressed in renal cell carcinoma cell lines, a tumor-specific CTL clone was established from tumor-infiltrating lymphocytes from a regressing pulmonary lesion. This CTL recognized nonmutated fibroblast growth factor-5 (FGF-5). Quantitative real-time reverse transcription PCR revealed that FGF-5 was overexpressed in the majority of renal cell carcinomas, as well as in some prostate carcinoma and breast carcinoma lines. FGF-5 expression by quantitative real-time reverse transcription PCR in normal tissues was below the recognition threshold for this CTL. As a normal protein with significant overexpression by multiple adenocarcinomas and little normal tissue expression, FGF-5 represents an immunotherapy target with potential utility against a broad array of nonmelanoma cancers.
Hanada
K
K
Surgery Branch, National Cancer Institute, NIH, Bethesda, Maryland 20892, USA.
Perry-Lalley
D M
DM
Ohnmacht
G A
GA
Bettinotti
M P
MP
Yang
J C
JC
D000230:Adenocarcinoma / Q000276:immunology* / Q000473:pathology; D000328:Adult; D000818:Animals; D000906:Antibodies / Q000276:immunology; D019556:COS Cells; D002292:Carcinoma, Renal Cell / Q000276:immunology / Q000473:pathology; D002999:Clone Cells; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D051521:Fibroblast Growth Factor 5; D005346:Fibroblast Growth Factors / Q000235:genetics / Q000276:immunology*; D006801:Humans; D009362:Neoplasm Metastasis / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014407:Tumor Cells, Cultured
D000906:Antibodies; D018076:DNA, Complementary; C496449:FGF5 protein, human; D051521:Fibroblast Growth Factor 5; D005346:Fibroblast Growth Factors