title: Rapid micro-assay of camptothecin in Camptotheca acuminata.
abstract: A micro-assay has been developed to extract and rapidly quantify the anticancer alkaloid, camptothecin (CPT), from two leaf disks of Camptotheca acuminata Decaisne (Nyssaceae). This assay utilizes thin-layer chromatography in conjunction with fluorescence imaging to obtain reproducible measurements in the nanogram range. A large number of trees can be screened using this procedure to identify high producers of CPT in a relatively short period of time.
authors.forename: B A
authors.initials: BA
authors.lastname: Nolte
authors.forename: R D
authors.initials: RD
authors.lastname: Lineberger
authors.forename: D W
authors.initials: DW
authors.lastname: Reed
authors.forename: M E
authors.initials: ME
authors.lastname: Rumpho
mesh_terms: D000470:Alkaloids / Q000032:analysis* / Q000302:isolation & purification*; D000972:Antineoplastic Agents, Phytogenic / Q000032:analysis* / Q000302:isolation & purification*; D002166:Camptothecin / Q000032:analysis* / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D004264:DNA Topoisomerases, Type I / Q000378:metabolism; D008752:Methylene Chloride; D010936:Plant Extracts / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*
chemical_list: D000470:Alkaloids; D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts; D008752:Methylene Chloride; D004264:DNA Topoisomerases, Type I; D002166:Camptothecin