title: The MGEA6 multigene family has an active locus on 14q and at least nine pseudogenes on different chromosomes.
abstract: The meningioma expressed antigen-6 (MGEA6) was originally identified as an immunogenic antigen in meningioma patients. Somatic hybrid panel mapping and fluorescence in situ hybridization revealed MGEA6-related sequences on different human chromosomes. Here we carry out database analysis to investigate the complexity of the MGEA6-related sequences and demonstrate the existence of a multigene family. We localized the active gene (spanning over 83 kb) to chromosome 14q and elucidated its exon/intron structure. We identified and characterized 9 processed pseudogenes on 9 different chromosomes including chromosomes 2, 3, 6, 7, 9, 10, 12, 13, and 18. We performed phylogenetic analysis and concluded that the MGEA6 pseudogenes may result from more than one retrotransposition event; we calculated divergence times of the pseudogenes to be between 21.5 and 28.9 million years ago.
authors.affiliation: Institut für Humangenetik, Universitätskliniken des Saarlandes, 66421 Homburg/Saar, Germany.
authors.forename: N
authors.initials: N
authors.lastname: Comtesse
authors.forename: K
authors.initials: K
authors.lastname: Reus
authors.forename: E
authors.initials: E
authors.lastname: Meese
mesh_terms: D017398:Alternative Splicing; D000951:Antigens, Neoplasm; D002874:Chromosome Mapping; D002883:Chromosomes, Human, Pair 14; D016208:Databases, Factual; D019143:Evolution, Molecular; D005091:Exons; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007438:Introns; D008969:Molecular Sequence Data; D005810:Multigene Family; D009363:Neoplasm Proteins / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D011544:Pseudogenes; D012333:RNA, Messenger / Q000378:metabolism; D018626:Retroelements / Q000235:genetics; D013997:Time Factors
chemical_list: D000951:Antigens, Neoplasm; C109278:MIA2 protein, human; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D018626:Retroelements