title: Transport of levofloxacin in the OK kidney epithelial cell line: interaction with p-aminohippurate transport.
abstract: OBJECTIVE
  To evaluate the mechanism of renal transport of quinolone antibacterial drugs, we examined the interaction of levofloxacin with p-aminohippurate (PAH) transport systems and the transport of levofloxacin in renal epithelial cells.
  
  
  METHODS
  Transport of [14C]PAH or [14C]levofloxacin was measured using OK cell monolayers grown on microporous membrane filters.
  
  
  RESULTS
  Transcellular transport from the basolateral to the apical side and cellular accumulation of [14C]PAH were inhibited by levofloxacin. Both the initial uptake of [14C]PAH from the basolateral side and the efflux to the apical side were inhibited by levofloxacin. The basolateral-to-apical transcellular transport of [14C]levofloxacin was greater than that in the opposite direction. [14C]Levofloxacin efflux to the apical side was greater than that to the basolateral side. Unlabeled levofloxacin and grepafloxacin inhibited the transcellular transport of [14C]levofloxacin, accompanied by an increase of cellular accumulation. However, neither PAH nor an anion transport inhibitor 4-4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS) affected the basolateral-to-apical transport of [14C]levofloxacin nor its uptake from the basolateral side.
  
  
  CONCLUSIONS
  These results indicated that levofloxacin inhibits PAH transport across both the basolateral and apical membranes of OK cells, but are not transported via the systems for PAH transport. The existence of a specific transport system for quinolones was indicated in OK cells.
authors.affiliation: Department of Pharmacy, Kyoto University Hospital, Faculty of Medicine, Kyoto University, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Matsuo
authors.forename: I
authors.initials: I
authors.lastname: Yano
authors.forename: Y
authors.initials: Y
authors.lastname: Habu
authors.forename: T
authors.initials: T
authors.lastname: Katsura
authors.forename: Y
authors.initials: Y
authors.lastname: Hashimoto
authors.forename: K
authors.initials: K
authors.lastname: Inui
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000378:metabolism*; D001693:Biological Transport, Active; D002460:Cell Line; D004847:Epithelial Cells / Q000378:metabolism*; D007668:Kidney / Q000378:metabolism*; D007700:Kinetics; D064704:Levofloxacin; D015242:Ofloxacin / Q000378:metabolism*; D010130:p-Aminohippuric Acid / Q000378:metabolism*
chemical_list: D000890:Anti-Infective Agents; D064704:Levofloxacin; D015242:Ofloxacin; D010130:p-Aminohippuric Acid