Protein determinants of insertional specificity for the Drosophila gypsy retrovirus.
The gypsy retrovirus invades the germ line of Drosophila females, inserting with a high frequency into the ovo locus. Gypsy insertion sites in ovo are clustered within a region in the promoter of the ovo gene that contains multiple binding sites for the OvoA and OvoB proteins. We found that a 1.3-kb DNA fragment containing this region is able to confer gypsy insertional specificity independent of its genomic location. The frequency of gypsy insertions into the ovo gene is significantly lower in wild-type females than in ovoD1 females. In addition, gypsy insertions in ovoD1 females occur during most stages of germ-line development whereas insertions in wild-type females occur only in late stages. This pattern of temporally specific insertions, as well as the higher frequency of insertion in ovoD1 females, correlates with the presence of the OvoA or OvoD1 proteins. The results suggest that gypsy insertional specificity might be determined by the binding of the OvoA repressor isoform to the promoter region of the gene.
Labrador
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Department of Biology, The Johns Hopkins University, Baltimore, Maryland 21218, USA.
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D000818:Animals; D001483:Base Sequence; D004247:DNA; D004268:DNA-Binding Proteins / Q000235:genetics; D004330:Drosophila / Q000821:virology*; D029721:Drosophila Proteins; D005260:Female; D005854:Germ Cells; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D018626:Retroelements; D012190:Retroviridae / Q000502:physiology*; D014157:Transcription Factors / Q000235:genetics
D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D018626:Retroelements; D014157:Transcription Factors; C089512:ovo protein, Drosophila; D004247:DNA