Profiling of genes expressed in human monocytes and monocyte-derived dendritic cells using cDNA expression array.
Using a human cDNA expression array, we obtained expression profiles of 588 genes in CD14+ monocytes and monocyte-derived dendritic cells (DCs). Overall, 22 genes were upregulated, and nine genes were downregulated in DCs of both samples from two different individuals. Many of the genes that were upregulated in DCs encode proteins that are related to differentiation, cell structure, migration, termination of cell cycle as well as proliferation, e.g. tumour necrosis factor-alpha (TNF-alpha), tumour necrosis factor receptor II (TNFRII), thymosin beta-10, epithelial discoidin domain receptor 1, replication factor C, putative transcription factor DB1, alpha catenin, transforming growth factor-beta 1, prohibitin, p53-regulating protein and neu differentiation factor. Among the downregulated genes in DCs were genes that encode proteins of cell cycle regulation: mitotic growth and transcription activator, platelet-derived growth factor receptor-beta subunit, interleukin 2 receptor (IL-2R)-gamma subunit, IL-7R-alpha subunit, leucocyte interferon-gamma (IFN-gamma) and granulocyte-macrophage colony-stimulating factor receptor (GM-CSFR). Semi-quantitative reverse transcription-polymerase chain reaction method confirmed the upregulated expression levels in DCs for TNFRII, TNF-alpha, alpha catenin and downregulation of IFN-gamma, GM-CSFR on four different donor samples of DCs and monocytes. Moreover, our data show the presence of a 'switch-on' step for the TNF-alpha and TNFRII gene expression in immature DCs for further differentiation into mature DCs.
Lapteva
N
N
Department of Human Genetics, Graduate School of Medicine, University of Tokyo, Tokyo, Japan.
Ando
Y
Y
Nieda
M
M
Hohjoh
H
H
Okai
M
M
Kikuchi
A
A
Dymshits
G
G
Ishikawa
Y
Y
Juji
T
T
Tokunaga
K
K
D002453:Cell Cycle / Q000235:genetics; D002454:Cell Differentiation / Q000235:genetics; D003598:Cytoskeletal Proteins / Q000235:genetics; D016172:DNA Fingerprinting; D018076:DNA, Complementary / Q000032:analysis*; D004268:DNA-Binding Proteins / Q000235:genetics; D003713:Dendritic Cells / Q000502:physiology*; D005786:Gene Expression Regulation; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics; D015847:Interleukin-4 / Q000494:pharmacology; D007963:Leukocytes, Mononuclear / Q000502:physiology*; D018950:Lipopolysaccharide Receptors; D020890:Neuregulin-1 / Q000235:genetics; D000091402:Prohibitins; D011506:Proteins / Q000235:genetics; D016187:Receptors, Granulocyte-Macrophage Colony-Stimulating Factor / Q000235:genetics; D015375:Receptors, Interleukin-2 / Q000235:genetics; D020395:Receptors, Interleukin-7 / Q000235:genetics; D018124:Receptors, Tumor Necrosis Factor / Q000235:genetics; D051818:Replication Protein C; D012097:Repressor Proteins; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics; D051178:alpha Catenin; D020742:rhoA GTP-Binding Protein / Q000235:genetics
C495273:CTNNA1 protein, human; D003598:Cytoskeletal Proteins; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D018950:Lipopolysaccharide Receptors; D020890:Neuregulin-1; D000091402:Prohibitins; D011506:Proteins; D016187:Receptors, Granulocyte-Macrophage Colony-Stimulating Factor; D015375:Receptors, Interleukin-2; D020395:Receptors, Interleukin-7; D018124:Receptors, Tumor Necrosis Factor; D012097:Repressor Proteins; D014157:Transcription Factors; D014409:Tumor Necrosis Factor-alpha; C088395:VEZF1 protein, human; D051178:alpha Catenin; D015847:Interleukin-4; D007371:Interferon-gamma; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D051818:Replication Protein C; D020742:rhoA GTP-Binding Protein