title: The Golgi complex is a microtubule-organizing organelle.
abstract: We show that the Golgi complex can directly stimulate microtubule nucleation in vivo and in vitro and thus behaves as a potent microtubule-organizing organelle in interphase cells. With the use of nocodazole wash-out experiments in hepatic cells, we found that the occurrence of noncentrosomal, early stabilized microtubules is highly correlated with the subcellular localization of Golgi membranes. With the use of in vitro reconstituted microtubule assembly systems with or without cytosol, we also found that, in contrast to centrosomally attached microtubules, the distal ends of Golgi-attached microtubules are remotely stabilized in a way that requires additional cytosolic component(s). Finally, we demonstrate that Golgi-based microtubule nucleation is direct and involves a subset of gamma-tubulin bound to the cytoplasmic face of the organelle.
authors.affiliation: Laboratoire de Biochimie et de Biologie Cellulaire, EA 1595, Faculté de Pharmacie, 92296 Châtenay-Malabry Cedex, France.
authors.forename: K
authors.initials: K
authors.lastname: Chabin-Brion
authors.forename: J
authors.initials: J
authors.lastname: Marceiller
authors.forename: F
authors.initials: F
authors.lastname: Perez
authors.forename: C
authors.initials: C
authors.lastname: Settegrana
authors.forename: A
authors.initials: A
authors.lastname: Drechou
authors.forename: G
authors.initials: G
authors.lastname: Durand
authors.forename: C
authors.initials: C
authors.lastname: Poüs
mesh_terms: D000107:Acetylation; D002460:Cell Line; D018385:Centrosome / Q000378:metabolism / Q000502:physiology; D006056:Golgi Apparatus / Q000502:physiology*; D022781:Hepatocytes / Q000166:cytology / Q000187:drug effects; D006801:Humans; D008870:Microtubules / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D015739:Nocodazole / Q000494:pharmacology; D015388:Organelles; D014404:Tubulin / Q000378:metabolism
chemical_list: D014404:Tubulin; D015739:Nocodazole