Characterization of baboon (Papio hamadryas) milk proteins.
The major proteins of baboon milk were identified as beta-lactoglobulin (beta LG), alpha-lactalbumin (alpha LA), lysozyme, lactoferrin, casein, and albumin by immobiline isoelectric focusing, SDS-PAGE, immunoblotting of gels with rabbit antisera to human alpha LA, lysozyme, and albumin and bovine beta LG and casein, and N-terminal sequencing of proteins blotted from gels. The first 30 N-terminal residues of baboon beta LG are identical to those of macaque (Macaca fasicularis) beta LG except for a (D/N) polymorphism at residue 2. The complete cDNA sequence and derived amino acid composition of beta LG were elucidated using RT-PCR amplification of poly(A)+ mRNA purified from lactating mammary gland. Baboon beta LG consists of 168 amino acid residues (M(r) 20,750) and is the longest beta LG identified to date. beta LG and alpha LA polymorphisms with three (A, B, and C) and two (A and B) variants, respectively, were detected by immobiline IEF, pH 4-6, of individual baboon milk samples at varying stages of lactation.
Hall
A J
AJ
Australian Equine Blood Typing Research Laboratory, University of Queensland, Brisbane, Australia.
Masel
A
A
Bell
K
K
Halliday
J A
JA
Shaw
D C
DC
VandeBerg
J L
JL
D000418:Albumins / Q000032:analysis / Q000378:metabolism; D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001483:Base Sequence; D002364:Caseins / Q000032:analysis / Q000235:genetics; D002417:Cattle; D017931:DNA Primers / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D006863:Hydrogen-Ion Concentration; D015151:Immunoblotting; D007525:Isoelectric Focusing; D007526:Isoelectric Point; D007781:Lactoferrin / Q000032:analysis / Q000235:genetics; D007782:Lactoglobulins / Q000032:analysis / Q000235:genetics; D008892:Milk / Q000737:chemistry*; D008894:Milk Proteins / Q000032:analysis* / Q000235:genetics; D008969:Molecular Sequence Data; D009113:Muramidase / Q000032:analysis / Q000235:genetics; D010215:Papio; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012333:RNA, Messenger / Q000302:isolation & purification / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
D000418:Albumins; D000596:Amino Acids; D002364:Caseins; D017931:DNA Primers; D007782:Lactoglobulins; D008894:Milk Proteins; D012333:RNA, Messenger; D009113:Muramidase; D007781:Lactoferrin