Genomic identity of pyelonephritogenic Escherichia coli isolated from blood, urine and faeces of children with urosepsis.
Chromosomal genotypes of Escherichia coli isolates from blood, urine and faeces of infants with urosepsis were studied to find possible clonality of the isolates. The isolates were analysed by PCR for class I, II and III alleles of the pyelonephritis-associated adhesin gene papG. The macrorestriction profiles of the papG-positive isolates were analysed by pulsed-field gel electrophoresis and their O serogroups were determined. Genetically identical E. coli isolates from the blood, urine and faeces of the same infant were found in 8 of 10 infants. This finding confirmed the results of previous phenotypic studies that the reservoir of pyelonephritogenic E. coli is indeed the colon.
Jantunen
Maria E
ME
*Hospital for Children and Adolescents, University of Helsinki, PO Box 280, FIN-00290 Helsinki, †Laboratory of Enteric Pathogens, National Public Health Institute (KTL), Mannerheimintie 166, FIN-00280 Helsinki and ‡Department of Pediatrics, Tampere University Hospital, PO Box 2000, FIN-33521 Tampere, Finland.
Saxén
H
H
*Hospital for Children and Adolescents, University of Helsinki, PO Box 280, FIN-00290 Helsinki, †Laboratory of Enteric Pathogens, National Public Health Institute (KTL), Mannerheimintie 166, FIN-00280 Helsinki and ‡Department of Pediatrics, Tampere University Hospital, PO Box 2000, FIN-33521 Tampere, Finland.
Lukinmaa
Susanna
S
*Hospital for Children and Adolescents, University of Helsinki, PO Box 280, FIN-00290 Helsinki, †Laboratory of Enteric Pathogens, National Public Health Institute (KTL), Mannerheimintie 166, FIN-00280 Helsinki and ‡Department of Pediatrics, Tampere University Hospital, PO Box 2000, FIN-33521 Tampere, Finland.
Ala-Houhala
Marja
M
*Hospital for Children and Adolescents, University of Helsinki, PO Box 280, FIN-00290 Helsinki, †Laboratory of Enteric Pathogens, National Public Health Institute (KTL), Mannerheimintie 166, FIN-00280 Helsinki and ‡Department of Pediatrics, Tampere University Hospital, PO Box 2000, FIN-33521 Tampere, Finland.
Siitonen
Anja
A
*Hospital for Children and Adolescents, University of Helsinki, PO Box 280, FIN-00290 Helsinki, †Laboratory of Enteric Pathogens, National Public Health Institute (KTL), Mannerheimintie 166, FIN-00280 Helsinki and ‡Department of Pediatrics, Tampere University Hospital, PO Box 2000, FIN-33521 Tampere, Finland.
D018830:Adhesins, Escherichia coli / Q000235:genetics*; D000483:Alleles; D002675:Child, Preschool; D003106:Colon / Q000382:microbiology; D016521:Electrophoresis, Gel, Pulsed-Field; D004926:Escherichia coli / Q000145:classification / Q000235:genetics*; D004927:Escherichia coli Infections / Q000382:microbiology*; D005243:Feces / Q000382:microbiology; D005260:Female; D036781:Fimbriae Proteins; D005838:Genotype; D006801:Humans; D008297:Male; D010641:Phenotype; D011446:Prospective Studies; D011704:Pyelonephritis / Q000382:microbiology; D012703:Serotyping; D014552:Urinary Tract Infections / Q000382:microbiology*
D018830:Adhesins, Escherichia coli; C092426:PapG protein, E coli; D036781:Fimbriae Proteins