title: Analysis of mutations in the p53 tumor suppressor gene and Ki- and Ha-ras proto-oncogenes in hepatic tumors of European flounder (Platichthys flesus).
abstract: DNA from five tumors, three other-injured livers and two normal liver tissue samples from the European flounder were analyzed for mutations in exons 5-8 of the tumor suppressor gene p53 and at codons 12, 13 and 61 of Ha- and Ki-ras proto-oncogenes. No tumor-specific mutations were identified by direct sequencing and single-strand conformation polymorphism of these genes. A number of silent polymorphisms were noted in p53. In addition to a need for more extensive analyses of flounder liver tumor samples for ras or p53 mutations, other cancer-related genes should be investigated.
authors.affiliation: School of Biosciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK.
authors.forename: T M
authors.initials: TM
authors.lastname: Franklin
authors.forename: J S
authors.initials: JS
authors.lastname: Lee
authors.forename: A
authors.initials: A
authors.lastname: Köhler
authors.forename: J K
authors.initials: JK
authors.lastname: Chipman
mesh_terms: D000818:Animals; D005091:Exons; D005393:Fish Diseases / Q000235:genetics*; D005432:Flounder; D011905:Genes, ras / Q000235:genetics*; D008113:Liver Neoplasms / Q000235:genetics / Q000662:veterinary*; D009154:Mutation; D011110:Polymorphism, Genetic; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*
chemical_list: D016159:Tumor Suppressor Protein p53