title: Detection of Mycoplasma bovis in preservative-treated field milk samples.
abstract: Control of mycoplasmal mastitis requires individual cow milk sampling for culture and identification of Mycoplasma bovis. This sampling is time-consuming and expensive. Currently, some herds sample cows monthly with the dairy herd improvement (DHI) program, but a preservative is added to this milk that kills M. bovis. In this paper, a nested polymerase chain reaction (PCR) procedure that allows for rapid testing of preservative-treated milk is validated. The specificity of the nested PCR assay was confirmed by testing isolated nucleic acids of other organisms phylogenetically related to M. bovis or common to milk. A comparison against blind-passage culture on 53 field milk samples determined its sensitivity. Exposure of seeded milk samples to the procedure resulted in a sensitivity of 5.1 cfu equivalents per milliliter. Analysis of these results proved that the nested PCR assay was as sensitive as traditional culture and can be used on preservative-treated milk.
authors.affiliation: Microbiology Research and Development, Hospital Products Division, Abbott Laboratories, Abbott Park, IL 60064, USA.
authors.forename: C C
authors.initials: CC
authors.lastname: Pinnow
authors.forename: J A
authors.initials: JA
authors.lastname: Butler
authors.forename: K
authors.initials: K
authors.lastname: Sachse
authors.forename: H
authors.initials: H
authors.lastname: Hotzel
authors.forename: L L
authors.initials: LL
authors.lastname: Timms
authors.forename: R F
authors.initials: RF
authors.lastname: Rosenbusch
mesh_terms: D000818:Animals; D002417:Cattle; D004269:DNA, Bacterial / Q000032:analysis; D005260:Female; D005520:Food Preservatives / Q000008:administration & dosage*; D008414:Mastitis, Bovine / Q000175:diagnosis* / Q000382:microbiology; D008892:Milk / Q000382:microbiology*; D009174:Mycoplasma / Q000302:isolation & purification*; D009175:Mycoplasma Infections / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D004269:DNA, Bacterial; D005520:Food Preservatives