Changes in the expression of tachykinin receptors in the rat uterus during the course of pregnancy.
In the mammalian female reproductive tract, tachykinin neuropeptides, such as substance P (SP), are localized to a population of sensory fibers and their precise physiological role is still unknown. The aim of the present study was to characterize the population of tachykinin receptors in the pregnant rat uterus and to assess their regulation during the course of pregnancy and after delivery. The expression of the tachykinin NK(1) receptor (NK(1)R), the tachykinin NK(2) receptor (NK(2)R), and the tachykinin NK(3) receptor (NK(3)R) in uteri from rats at different stages of pregnancy and on Day 1 postpartum was investigated by using a semiquantitative reverse transcription-polymerase chain reaction. The contractile effect of tachykinin receptor agonists acting selectively on the NK(1)R, the NK(2)R, or the NK(3)R was investigated by conventional organ bath techniques. Serum levels of estrogen and progesterone were measured by RIA. Our data show that the expression and function of NK(1)R and NK(3)R varied along the course of pregnancy and at postpartum. Uterine NK(2)R mRNA levels remain stable during the course of pregnancy and at Day 1 postpartum; and the contractions elicited by activating selectively the NK(2) receptor in the presence of the neutral endopeptidase inhibitor phosphoramidon (1 microM) were similar in early, mid, or late pregnancy. These results show that the expression and function of tachykinin receptors within the uterus vary with reproductive state and length of gestation, supporting a role for tachykinins in pregnancy and/or parturition in the rat.
Candenas
M L
ML
Instituto de Investigaciones Químicas, Centro de Investigaciones Científicas Isla de la Cartuja, 41092 Sevilla, Spain. mluz@cica.es
Magraner
J
J
Armesto
C P
CP
Anselmi
E
E
Nieto
P M
PM
Martín
J D
JD
Advenier
C
C
Pinto
F M
FM
D000818:Animals; D004958:Estradiol / Q000097:blood; D005260:Female; D015870:Gene Expression; D006020:Glycopeptides / Q000494:pharmacology; D010880:Piperidines / Q000494:pharmacology; D011247:Pregnancy; D011374:Progesterone / Q000097:blood; D011812:Quinuclidines / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D018040:Receptors, Neurokinin-1 / Q000235:genetics; D018041:Receptors, Neurokinin-2 / Q000235:genetics; D018042:Receptors, Neurokinin-3 / Q000235:genetics; D018039:Receptors, Tachykinin / Q000819:agonists / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014590:Uterine Contraction / Q000187:drug effects; D014599:Uterus / Q000737:chemistry* / Q000502:physiology
D006020:Glycopeptides; D010880:Piperidines; D011812:Quinuclidines; D018040:Receptors, Neurokinin-1; D018041:Receptors, Neurokinin-2; D018042:Receptors, Neurokinin-3; D018039:Receptors, Tachykinin; C085215:SR 140333; D011374:Progesterone; D004958:Estradiol; C008890:phosphoramidon