title: Identification of larval-stage antigens of ascaris suum recognized with immune sera from pigs.
abstract: Antigens were identified from the third-stage larvae (L3) and lung-stage larvae of Ascaris suum by two-dimensional immunoblot method with antisera obtained from pigs that received chemically abbreviated Ascaris suum larval infections. Forty-seven and 13 spots were recognized as antigens from the L3 and lung-stage larvae, respectively. Their apparent molecular weight ranged from 20 to 101 kDa and their isoelectric point from 3.6 to 8.0. The present study provides a framework for further molecular cloning of those antigens and consequently leads to the development of recombinant peptide vaccines against A. suum.
authors.affiliation: Laboratory of Parasitic Diseases, National Institute of Animal Health, Tsukuba, Ibaraki, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Kasug-Aoki
authors.forename: N
authors.initials: N
authors.lastname: Tsuji
authors.forename: K
authors.initials: K
authors.lastname: Suzuki
authors.forename: T
authors.initials: T
authors.lastname: Arakawa
authors.forename: Y
authors.initials: Y
authors.lastname: Matsumoto
authors.forename: T
authors.initials: T
authors.lastname: Isobe
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis*; D001196:Ascariasis / Q000097:blood / Q000276:immunology / Q000469:parasitology / Q000662:veterinary*; D017165:Ascaris suum / Q000254:growth & development* / Q000276:immunology; D015153:Blotting, Western / Q000662:veterinary; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D005260:Female; D007525:Isoelectric Focusing / Q000662:veterinary; D008297:Male; D013047:Specific Pathogen-Free Organisms; D013552:Swine; D013553:Swine Diseases / Q000097:blood / Q000276:immunology / Q000469:parasitology*
chemical_list: D000947:Antigens, Helminth