title: Classification of trypanosomatids from fruits and seeds using morphological, biochemical and molecular markers revealed several genera among fruit isolates.
abstract: Trypanosomatids are widespread in several plant families and although most isolates have been classified as Phytomonas, other trypanosomatid genera can also infect plants. In order to assess the natural occurrence of non-Phytomonas trypanosomatids in plants we characterized 21 new trypanosomatid cultures, 18 from fruits and three from seeds of 17 plant species. The trypanosomatids from fruit and seeds were compared in terms of morphological, growth, biochemical and molecular features. The high diversity among the isolates permitted the classification of the new flagellates into the genera Crithidia and Leptomonas as well as Phytomonas. The data showed that natural fruit infection with non-Phytomonas trypanosomatids is more common than usually thought, being detected in 43% of the fruit isolates.
authors.affiliation: Departamento de Microbiologia, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Paraná, Brazil.
authors.forename: L M
authors.initials: LM
authors.lastname: Catarino
authors.forename: M G
authors.initials: MG
authors.lastname: Serrano
authors.forename: M
authors.initials: M
authors.lastname: Cavazzana
authors.forename: M L
authors.initials: ML
authors.lastname: Almeida
authors.forename: E K
authors.initials: EK
authors.lastname: Kaneshina
authors.forename: M
authors.initials: M
authors.lastname: Campaner
authors.forename: J V
authors.initials: JV
authors.lastname: Jankevicius
authors.forename: M M
authors.initials: MM
authors.lastname: Teixeira
authors.forename: S
authors.initials: S
authors.lastname: Itow-Jankevicius
mesh_terms: D000818:Animals; D003421:Crithidia / Q000145:classification / Q000166:cytology / Q000302:isolation & purification / Q000502:physiology; D016172:DNA Fingerprinting; D019084:Fluorescent Antibody Technique, Indirect; D005638:Fruit / Q000469:parasitology*; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D019105:Random Amplified Polymorphic DNA Technique; D021521:Ribotyping; D012639:Seeds / Q000469:parasitology*; D014351:Trypanosomatina / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification / Q000502:physiology