The lipopolysaccharides of the phytopathogen Xanthomonas campestris pv. campestris induce an oxidative burst reaction in cell cultures of Nicotiana tabacum.
The lipopolysaccharides (LPSXcc) of the phytopathogenic bacteria Xanthomonas campestris pv. campestris (X.c.c.) were purified from an exopolysaccharide-deficient mutant strain. The isolated LPSxcc induced an oxidative burst reaction in cell-suspension cultures of the non-host plant tobacco (Nicotiana tabacum L.) SRI. The oxidative burst elicited by LPSXcc differed from that induced by yeast elicitor (YE), a cell wall preparation of baker's yeast. The LPSXcc-induced oxidative burst was characterised by a slow increase in H2O2 production and an extended decline. Both the LPSXcc-and YE-induced oxidative bursts were completely blocked by the NAD(P)H-oxidase inhibitor diphenylene-iodonium. When LPSXcc and YE were applied in combination, a synergistic effect and the establishment of refractory states in the generation of H2O2 were observed. The amount of cytosolic calcium was measured in transgenic tobacco cell cultures carrying the apoaequorin gene by coelenterazine-derived chemiluminescence. Whereas YE induced a calcium peak within 1 min after application, LPSXcc induced a long-term calcium signal without transients. To our knowledge this is the first report on the elicitation of an oxidative burst in plant cell cultures by isolated LPS of a phytopathogenic bacterium.
Meyer
A
A
Universität Bielefeld, Fakultät für Biologie, Lehrstuhl für Genetik, Germany.
Pühler
A
A
Niehaus
K
K
D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D006861:Hydrogen Peroxide / Q000378:metabolism; D008070:Lipopolysaccharides / Q000276:immunology / Q000302:isolation & purification / Q000494:pharmacology*; D019255:NADPH Oxidases / Q000037:antagonists & inhibitors; D009861:Onium Compounds / Q000494:pharmacology; D010935:Plant Diseases / Q000382:microbiology*; D030821:Plants, Genetically Modified; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis; D016897:Respiratory Burst; D012441:Saccharomyces cerevisiae; D015398:Signal Transduction; D014026:Nicotiana / Q000382:microbiology*; D016959:Xanthomonas campestris / Q000276:immunology / Q000502:physiology*
D008070:Lipopolysaccharides; D009861:Onium Compounds; D011135:Polysaccharides, Bacterial; C007517:diphenyleneiodonium; D006861:Hydrogen Peroxide; D019255:NADPH Oxidases; D002118:Calcium