title: Polo-like kinase is required for the fragmentation of pericentriolar Golgi stacks during mitosis.
abstract: The pericentriolar stacks of Golgi cisternae undergo extensive reorganization during mitosis in mammalian cells. GM130 and GRASP65 (Golgi reassembly stacking protein of 65 kDa) are Golgi-associated proteins that are targets of mitotic kinases, and they have also been implicated in the reorganization of the Golgi structure during cell division. Previous studies have reported that mitogen-activated protein kinase kinase-1 (MEK1) and Cdc2 protein kinases are involved in these dynamic changes in the Golgi structure. More recently, the mitotic polo-like kinase (Plk) has been shown to interact with and phosphorylate GRASP65. Here, we provide evidence that Plk is involved in the mitosis-specific fragmentation of the Golgi apparatus. The addition of kinase-defective Plk or immunodepletion of Plk disrupts the fragmentation process. Furthermore, Golgi fragmentation is inhibited by the addition of either full-length or truncated GRASP65. These findings suggest that phosphorylation of GRASP65 by Plk may be a critical event in the reorganization of the Golgi structure during mitosis.
authors.affiliation: Biology Department, University of California at San Diego, La Jolla, CA 92093, USA.
authors.forename: C
authors.initials: C
authors.lastname: Sütterlin
authors.forename: C Y
authors.initials: CY
authors.lastname: Lin
authors.forename: Y
authors.initials: Y
authors.lastname: Feng
authors.forename: D K
authors.initials: DK
authors.lastname: Ferris
authors.forename: R L
authors.initials: RL
authors.lastname: Erikson
authors.forename: V
authors.initials: V
authors.lastname: Malhotra
mesh_terms: D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D002502:Centrioles / Q000648:ultrastructure; D017931:DNA Primers; D029721:Drosophila Proteins; D006056:Golgi Apparatus / Q000201:enzymology / Q000648:ultrastructure*; D000077614:Golgi Matrix Proteins; D008565:Membrane Proteins / Q000378:metabolism; D008938:Mitosis; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D051381:Rats
chemical_list: D017931:DNA Primers; D029721:Drosophila Proteins; D000077614:Golgi Matrix Proteins; C491577:Gorasp1 protein, rat; D008565:Membrane Proteins; D017346:Protein Serine-Threonine Kinases