[Effect of inhibitors of enzymatic DNA methylation on the formation of reproductive structures and carotenoid production in Neurospora crassa].
The effect of inhibitors of DNA methylation on light-sensitive developmental stages of the filamentous fungus Neurospora crassa was studied. Under conditions of nitrogen starvation, when blue light induced protoperithecia development and inhibited conidia formation, 5-azacytidine (3-300 microM) inhibited protoperithecia formation and stimulated conidia formation (a 700-fold increase after light induction). After treatment of the mycelium with 5-azacytidine, the protoperithecia formation was accompanied by inversely proportional changes in the formation of conidia, both in the dark and after illumination. In the mycelium cultivated on the Vogel's medium, 5-azacytidine (up to 30 microM) and methotrexate (up to 3 microM) stimulated the light-induced carotenoid synthesis by 30%, whereas higher concentrations of these agents were toxic to carotenoid synthesis and growth.
Kritskiĭ
M S
MS
Bach Institute of Biochemistry, Russian Academy of Sciences, Leninskii pr. 33, Moscow, 117071 Russia.
Filippovich
S Iu
SIu
Afanas'eva
T P
TP
Bachurina
G P
GP
Russo
V E
VE
D001374:Azacitidine / Q000494:pharmacology*; D002338:Carotenoids / Q000096:biosynthesis*; D019175:DNA Methylation / Q000187:drug effects*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D008727:Methotrexate / Q000494:pharmacology*; D009492:Neurospora crassa / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism
D004791:Enzyme Inhibitors; D002338:Carotenoids; D001374:Azacitidine; D008727:Methotrexate