title: Isolation of a non-haemadsorbing, non-cytopathic strain of African swine fever virus in Madagascar.
abstract: African swine fever (ASF) suspected clinically in Madagascar (1998-9) was confirmed by polymerase chain reaction (PCR) and nucleotide sequencing, following virus isolation. No haemadsorption or cytopathic effect could be detected following leukocyte inoculation, but viral growth in cells was confirmed by PCR. Detection of ASF virus genome was carried out by amplification of a highly conserved region coding for the p72 protein. Nucleotide sequencing of the amplicon revealed 99.2% nucleotide identity between the recent Malagasy strains and a virus recovered from the 1994 outbreak in Mozambique (SPEC265). A serological survey performed on 449 sera, revealed that only 5.3% of the sera taken from pigs between 1998 and 1999 were positive.
authors.affiliation: AFSSA-Lerpaz, Maisons-Alfort, France.
authors.forename: M
authors.initials: M
authors.lastname: Gonzague
authors.forename: F
authors.initials: F
authors.lastname: Roger
authors.forename: A
authors.initials: A
authors.lastname: Bastos
authors.forename: C
authors.initials: C
authors.lastname: Burger
authors.forename: T
authors.initials: T
authors.lastname: Randriamparany
authors.forename: S
authors.initials: S
authors.lastname: Smondack
authors.forename: C
authors.initials: C
authors.lastname: Cruciere
mesh_terms: D000357:African Swine Fever / Q000453:epidemiology* / Q000821:virology*; D000358:African Swine Fever Virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004279:DNA, Viral / Q000235:genetics*; D004196:Disease Outbreaks / Q000706:statistics & numerical data*; D008270:Madagascar / Q000453:epidemiology; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011159:Population Surveillance; D016415:Sequence Alignment; D012703:Serotyping; D013552:Swine
chemical_list: D004279:DNA, Viral