Isolation of a chromosomally engineered durum wheat line carrying the Aegilops ventricosa pchl gene for resistance to eyespot.
The chromosome 7Dv of Aegilops ventricosa (syn. Triticum ventricosum, 2n = 4x = 28, genome DvDvMvMv) carries the gene Pch1 for resistance to eyespot. This gene has previously been transferred to chromosome 7D of bread wheat, T. aestivum (2n = 6x = 42, genome AABBDD). To (1) enhance the level of resistance of bread wheat by increasing the copy number of Pch1, and (2) create eyespot-resistant triticales, meiotically stable Pch1-carrying durum lines were selected from the backcross progenies of a cross between Ae. ventricosa and T. durum cv. Creso ph1c (2n = 4x = 28, genome AABB). The Pch1 transfer, likely resulting from homoeologous recombination, was located at the distal position on the long arm of chromosome 7A. The 7A microsatellite marker Xgwm 698 was found closely linked in repulsion to the introgression in the resistant recombination lines, and the endopeptidase allele located on chromosome 7A of cv. Creso ph1c was lost.
Huguet-Robert
V
V
Institut National de la Recherche Agronomique Station d'Amelioration des Plantes, Le Rheu, France.
Dedryver
F
F
Röder
Korzun
V
V
Abélard
P
P
Tanguy
A M
AM
Jaudeau
B
B
Jahier
J
J
D020090:Chromosome Segregation / Q000235:genetics; D002875:Chromosomes / Q000235:genetics*; D017343:Genes, Plant / Q000235:genetics*; D005818:Genetic Engineering; D020022:Genetic Predisposition to Disease; D019684:Magnoliopsida / Q000235:genetics*; D008540:Meiosis / Q000235:genetics; D018895:Microsatellite Repeats / Q000235:genetics; D010641:Phenotype; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology; D030821:Plants, Genetically Modified; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012689:Sequence Homology, Nucleic Acid; D014170:Transformation, Genetic; D019076:Transgenes / Q000235:genetics; D014908:Triticum / Q000166:cytology / Q000235:genetics* / Q000382:microbiology