Immunoelectron microscopic localization of partially purified antigens in adult Paragonimus iloktsuenesis.
An immunoelectron microscopy employing immunogold labeling method was performed to detect tissue origin of D1 fraction (D1A) among 5 antigenic protein fractions partially purified by DEAE-anion exchange chromatography from water-soluble crude antigen (PIWA) of adult Paragonimus iloktsuenensis. Immune reactions of adult worm tissues with rabbit serum immunoglobulin immunized with crude antigen (PI-Ig) and D1 antigen (D1-Ig), as well as rat serum immunoglobulin infected with P. iloktsuenensis were observed. D1A showed strong antigenicity in the intestinal epithelium of the worms during the early infection period of 2-4 weeks after infection. The vitellaria also showed stronger antigenicity than the other tissue sites in immune reaction of tissues against all immunoglobulins from 4 to 33 weeks after vitelline development. Therefore, it is suggested that D1A was mainly originated from the intestinal epithelial tissues before the development of vitelline gland of the parasites. Immuno-reactivity of two immunoglobulins (PI-Ig, D1-Ig) was significantly different in intestinal epithelial cytoplasmic protrusions (CP) and intestinal epithelial secretory granules (SG). In the experimental group with D1-Ig, gold particles were labeled significantly in CP than in SG when compared to the PI-Ig group. Thus, the major antigenic materials in D1 antigen having a strong antigenicity in the early infection period was considered to be originated from the intestinal epithelial tissue.
Lee
O R
OR
Department of Parasitology, Soonchunghyang University College of Medicine, Chonan 300-090, Korea. leeokran@sch.ac.kr
Chung
P R
PR
D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis* / Q000302:isolation & purification; D002852:Chromatography, Ion Exchange; D007150:Immunohistochemistry / Q000379:methods; D016253:Microscopy, Immunoelectron; D010238:Paragonimus / Q000276:immunology*
D000947:Antigens, Helminth