Effect of dietary cowpea trypsin inhibitor (CpTI) on the growth and development of the tomato moth Lacanobia oleracea (Lepidoptera: Noctuidae) and on the success of the gregarious ectoparasitoid Eulophus pennicornis (Hymenoptera: Eulophidae).
Cowpea trypsin inhibitor (CpTI) was shown to have a deleterious effect on the growth and development of larvae of the tomato moth, Lacanobia oleracea, when incorporated in artificial diet (2.0% of soluble protein) and expressed in transgenic potato leaf (up to 1.0% of soluble protein). The effect of CpTI on parasitism of L oleracea by the ectoparasitoid Eulophus pennicornis was investigated. The parasitic success of the wasp was reduced by the presence of CpTI in the diet of the host and, in the case of transgenic potato leaves expressing the transgene protein, was collated with the length of time the host fed on the diet prior to parasitism. In all cases the proportion of hosts parasitised when fed CpTI-containing diets was reduced when compared with controls, although these differences were only significant when hosts were fed from the third instar on the transgenic potato leaves. Parasitoid progeny that developed on L oleracea reared on CpTI-containing diets, however, were not adversely affected. These results show that, whilst expression of CpTI in transgenic potato plants confers resistance to the lepidopterous pest L oleracea, adverse effects on the ability of the ectoparasitoid E pennicornis to parasitise this moth species successfully may also occur. These results are discussed in relation to the potential impact of transgenic crops on beneficial biological control agents.
Bell
H A
HA
Central Science Laboratory, Sand Hutton, York YO41 1LZ, UK. h.bell@csl.gov.uk
Fitches
E C
EC
Down
R E
RE
Ford
L
L
Marris
G C
GC
Edwards
J P
JP
Gatehouse
J A
JA
Gatehouse
A M
AM
D000818:Animals; D001835:Body Weight; D004032:Diet; D007887:Fabaceae / Q000737:chemistry; D006790:Host-Parasite Interactions / Q000502:physiology; D006927:Hymenoptera / Q000187:drug effects* / Q000254:growth & development; D007300:Insect Control; D007306:Insecticides / Q000378:metabolism / Q000494:pharmacology*; D007814:Larva / Q000254:growth & development / Q000469:parasitology; D007915:Lepidoptera / Q000187:drug effects* / Q000254:growth & development / Q000469:parasitology; D010572:Pest Control, Biological; D010940:Plant Proteins / Q000009:adverse effects; D030821:Plants, Genetically Modified / Q000009:adverse effects; D010946:Plants, Medicinal; D018570:Risk Assessment; D014361:Trypsin Inhibitors / Q000378:metabolism / Q000494:pharmacology*
D007306:Insecticides; D010940:Plant Proteins; D014361:Trypsin Inhibitors