Association of chondroadherin with collagen type II.
Chondroadherin is a cell binding, leucine-rich repeat protein found in the territorial matrix of articular cartilage. Several members of the leucine-rich repeat protein family present in the extracellular matrix of e.g. cartilage have been shown to interact with collagen and influence collagen fibrillogenesis. We show that complexes of monomeric collagen type II and chondroadherin can be released under non-denaturing conditions from articular cartilage treated with p-aminophenylmercuric acetate to activate resident matrix metalloproteinases. Purified complexes as well as complexes formed in vitro between recombinant chondroadherin and collagen type II were studied by electron microscopy. Chondroadherin was shown to bind to two sites on collagen type II. The interaction was characterized by surface plasmon resonance analysis showing K(D) values in the nanomolar range. Both chondroadherin and collagen interact with chondrocytes, partly via the same receptor, but give rise to different cellular responses. By also interacting with each other, a complex system is created which may be of functional importance for the communication between the cells and its surrounding matrix and/or in the regulation of collagen fibril assembly.
Mansson
B
B
Department of Cell and Molecular Biology, Section for Connective Tissue Biology, Lund University, BMC, C12, SE-221 84 Lund, Sweden.
Wenglén
C
C
Mörgelin
M
M
Saxne
T
T
Heinegård
D
D
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D015153:Blotting, Western; D002356:Cartilage / Q000648:ultrastructure; D002417:Cattle; D019902:Chondrocytes / Q000502:physiology / Q000648:ultrastructure; D003001:Cloning, Molecular; D003094:Collagen / Q000737:chemistry* / Q000302:isolation & purification / Q000648:ultrastructure*; D004591:Electrophoresis, Polyacrylamide Gel; D016326:Extracellular Matrix Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000648:ultrastructure*; D015723:Gene Library; D006801:Humans; D007700:Kinetics; D008969:Molecular Sequence Data
D000906:Antibodies; D016326:Extracellular Matrix Proteins; C088980:chondroadherin; D003094:Collagen