Sensitive determination of metal ions by liquid chromatography with tris(2,2'-bipyridine) ruthenium (II) complex electrogenerated chemiluminescence detection.
Emetine dithiocarbamate metal complex, which is prepared from emetine, carbon disulfide, and metal (II), was found to indicate a large chemiluminescence intensity on the electrogenerated chemiluminescence of tris(2,2'-bipyridine)ruthenium(II). Liquid chromatography equipped with the chemiluminescence detection was developed for analyzing trace metal ions by use of the metal complex formation. The mixture of the Cu(II) and Co(II) complexes as a model sample was injected into the LC system. The two metal complexes and an excess emetine were successfully separated. The Cu(II) and Co(II) complexes were determined over the range 1-300 nM (the detection limit of 650 fg) and 30-5000 nM (the detection limit of 17 pg), respectively.
Tsukagoshi
K
K
Department of Chemical Engineering and Materials Science, Faculty of Engineering, Doshisha University, Tanabe, Kyoto, Japan. ktsukago@mail.doshisha.ac.jp
Miyamoto
K
K
Nakajima
R
R
Ouchiyam
N
N
D015082:2,2'-Dipyridyl / Q000031:analogs & derivatives* / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods*; D056831:Coordination Complexes; D008163:Luminescent Measurements; D008670:Metals / Q000032:analysis*; D012680:Sensitivity and Specificity; D017975:Ruthenium Compounds
D015082:2,2'-Dipyridyl; D056831:Coordination Complexes; D008670:Metals; C547232:tris(2,2-bipyridine)-ruthenium(II); D017975:Ruthenium Compounds