title: Herpes simplex virus type 2: a cause of acute retinal necrosis syndrome.
abstract: OBJECTIVE
  To describe a case of herpes simplex virus type 2 (HSV-2) acute retinal necrosis syndrome (ARN) in a 13-year-old immunocompetent girl.
  
  
  METHODS
  Polymerase chain reaction (PCR), cultures, flow cytometry, and cytology were performed on the vitreous sample.
  
  
  RESULTS
  Both PCR studies and vitreous cultures revealed HSV-2 as the cause of ARN. Flow cytometry showed CD4+, CD8+, and natural killer cells. The visual outcome of the patient was 20/200.
  
  
  CONCLUSIONS
  Successful culture of HSV-2 from the vitreous specimen in a patient with ARN proved HSV-2 to be one of the causes of ARN. The successful culture of HSV-2 has not been previously reported.
authors.affiliation: Department of Ophthalmology, General Hospital of Athens, Athens Medical School, Athens, Greece. marnik@otenet.gr
authors.forename: N N
authors.initials: NN
authors.lastname: Markomichelakis
authors.forename: P
authors.initials: P
authors.lastname: Zafirakis
authors.forename: P
authors.initials: P
authors.lastname: Karambogia-Karefillidi
authors.forename: N
authors.initials: N
authors.lastname: Drakoulis
authors.forename: E
authors.initials: E
authors.lastname: Vagiakou-Boudri
authors.forename: G
authors.initials: G
authors.lastname: Paterakis
authors.forename: M
authors.initials: M
authors.lastname: Apostolopoulos
mesh_terms: D000293:Adolescent; D000914:Antibodies, Viral / Q000032:analysis; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D004279:DNA, Viral / Q000032:analysis; D015828:Eye Infections, Viral / Q000175:diagnosis / Q000821:virology; D005260:Female; D005434:Flow Cytometry; D006558:Herpes Genitalis / Q000175:diagnosis / Q000821:virology; D018258:Herpesvirus 2, Human / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D007075:Immunoglobulin M / Q000032:analysis; D007694:Killer Cells, Natural / Q000276:immunology; D016133:Polymerase Chain Reaction; D012163:Retinal Detachment / Q000000981:diagnostic imaging / Q000821:virology; D015882:Retinal Necrosis Syndrome, Acute / Q000175:diagnosis / Q000821:virology*; D014463:Ultrasonography; D014792:Visual Acuity; D014822:Vitreous Body / Q000821:virology
chemical_list: D000914:Antibodies, Viral; D004279:DNA, Viral; D007075:Immunoglobulin M