Radiation hybrid mapping of 11 alpha and beta nicotinic acetylcholine receptor genes in Rattus norvegicus.
Acetylcholine is the main neurotransmitter of the vestibular efferents and a wide variety of muscarinic and nicotinic acetylcholine receptors are expressed in the vestibular periphery. To date, 11 nicotinic subunits (alpha and beta) have been reported in mammals. Previously, our group [Brain Res. 778 (1997) 409] reported that these nicotinic acetylcholine receptor alpha and beta subunits were differentially expressed in the vestibular periphery of the rat. To begin an understanding of the molecular genetics of these vestibular efferents, this study examined the chromosomal locations of these nicotinic acetylcholine receptor genes in the rat (Rattus norvegicus). Using radiation hybrid mapping and a rat radiation hybrid map server (www.rgd.mcw.edu/RHMAP SERVER/), we determined the chromosomal position for each of these genes. The alpha2-7, alpha9, alpha10, and beta2-4 nicotinic subunits mapped to the following chromosomes: alpha2, chr. 15; alpha3, chr. 8; alpha4, chr. 3; alpha5, chr. 8; alpha6, chr. 16; alpha7, chr. 1; alpha9, chr. 14; alpha10, chr. 7; beta2, chr. 2; beta3, chr. 16; and beta4, chr. 8. With the location for each of these nicotinic subunits known, it is now possible to develop consomic and/or congenic strains of rats that can be used to study the functional genomics of each of these subunits.
Tseng
J
J
Department of Otolaryngology and Communication Sciences, Medical College of Wisconsin, 9200 W. Wisconsin Ave., Milwaukee, WI 53226, USA.
Kwitek-Black
A E
AE
Erbe
C B
CB
Popper
P
P
Jacob
H J
HJ
Wackym
P A
PA
D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D017931:DNA Primers; D004525:Efferent Pathways / Q000502:physiology; D015870:Gene Expression / Q000502:physiology; D008969:Molecular Sequence Data; D021101:Radiation Hybrid Mapping; D051381:Rats; D011978:Receptors, Nicotinic / Q000235:genetics*; D014725:Vestibular Nerve / Q000502:physiology*; D014722:Vestibule, Labyrinth / Q000502:physiology*
D017931:DNA Primers; D011978:Receptors, Nicotinic