title: Detection of physiologically active compounds using cell-based biosensors.
abstract: Cell-based biosensors are portable devices that contain living biological cells that monitor physiological changes induced by exposure to environmental perturbations such as toxicants, pathogens or other agents. Methods of detecting physiological changes include extracellular electrical recordings, optical measurements, and, in the future, functional genomics and proteomics. Several technical developments are occurring that will increase the feasibility of cell-based biosensors for field applications; these developments include stem cell and 3D culture technologies. Possible scenarios for the use of cell-based biosensors include broad-range detectors of unknown threat agents and functional assessment of identified agents.
authors.affiliation: Center for BioMolecular Science and Engineering, Code 6910, Naval Research Laboratory, Washington, DC 20375, USA. das@cbmse.nrl.navy.mil
authors.forename: D A
authors.initials: DA
authors.lastname: Stenger
authors.forename: G W
authors.initials: GW
authors.lastname: Gross
authors.forename: E W
authors.initials: EW
authors.lastname: Keefer
authors.forename: K M
authors.initials: KM
authors.lastname: Shaffer
authors.forename: J D
authors.initials: JD
authors.lastname: Andreadis
authors.forename: W
authors.initials: W
authors.lastname: Ma
authors.forename: J J
authors.initials: JJ
authors.lastname: Pancrazio
mesh_terms: D015374:Biosensing Techniques; D009474:Neurons; D013234:Stem Cells