Separation of inosine, hypoxanthine, and guanosine by high-performance liquid chromatography on silica.
The separation of inosine (Ino), hypoxanthine (Hyp), and guanosine (Guo) on silica has been studied. In adsorption normal-phase systems the peak shapes are unsatisfactory; a low selectivity has been observed for the Ino-Hyp and Guo-Hyp pairs. Chromatograms can be significantly improved if systems with a mixed partition-adsorption retention mechanism are applied.
Kazoka
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Latvian Institute of Organic Synthesis, Riga.
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006151:Guanosine / Q000302:isolation & purification*; D019271:Hypoxanthine / Q000302:isolation & purification*; D007288:Inosine / Q000302:isolation & purification*; D012822:Silicon Dioxide; D013056:Spectrophotometry, Ultraviolet
D006151:Guanosine; D019271:Hypoxanthine; D007288:Inosine; D012822:Silicon Dioxide