Specific interactions between F1 adhesin of Streptococcus pyogenes and N-terminal modules of fibronectin.
Protein F1 is a surface protein of Streptococcus pyogenes that mediates high affinity binding to fibronectin (Fn) and facilitates S. pyogenes adherence and penetration into cells. The smallest portion of F1 known to retain the full binding potential of the intact protein is a stretch of 49 amino acids known as the functional upstream domain (FUD). Synthetic and recombinant versions of FUD were labeled with fluorescein isothiocyanate and used in fluorescence anisotropy experiments. These probes bound to Fn or the 70-kDa fragment of Fn with dissociation constants of 8-30 nm. Removal of the N-terminal seven residues of FUD did not cause a change in binding affinity. Further N- or C-terminal truncations resulted in complete loss of binding activity. Analysis of recombinant versions of the 70-kDa fragment that lacked one or several type I modules indicates that residues 1-7 of the 49-mer bind to type I modules I1 and I2 of the 27-kDa subfragment and the C-terminal residues bind to modules I4 and I5. Fluorescein isothiocyanate-labeled 49-mer also bound with lower affinity to large Fn fragments that lack the five type I modules of the 27-kDa fragment but contain the other seven type 1 modules of Fn. These results indicate that, although FUD has a general affinity for type I modules, high affinity binding of FUD to Fn is mediated by specific interactions with N-terminal type I modules.
Ensenberger
M G
MG
Department of Medicine and the Molecular and Cellular Pharmacology Program, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.
Tomasini-Johansson
B R
BR
Sottile
J
J
Ozeri
V
V
Hanski
E
E
Mosher
D F
DF
D018829:Adhesins, Bacterial / Q000378:metabolism*; D000595:Amino Acid Sequence; D001665:Binding Sites; D001667:Binding, Competitive; D005353:Fibronectins / Q000737:chemistry / Q000378:metabolism*; D006493:Heparin / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D010455:Peptides / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D013297:Streptococcus pyogenes / Q000378:metabolism*
D018829:Adhesins, Bacterial; D005353:Fibronectins; D010455:Peptides; D011994:Recombinant Proteins; D006493:Heparin