A sensitive method for the determination of gemfibrozil in human plasma samples by RP-LC.
A sensitive high-performance liquid chromatographic assay for the quantitative determination of gemfibrozil is described in this work. Ibuprofen was used as internal standard. The assay involved a single cyclohexane extraction and LC analysis with fluorescence detection. Chromatography was performed at 40 degrees C on a Hypersil ODS column. The mobile phase was a mixture of a solution of phosphoric acid 0.4% and acetonitrile (45:55). The method was validated. The detection limit of this method was 0.025 microg ml(-1); only 0.5 ml of the plasma sample was required for the determination. The calibration graph was linear from 0.05 to 0.5 microg ml(-1) and required a cubic equation from 0.5 to 30 microg ml(-1). Intra and inter-day precision (C.V.) did no exceed 15%. Mean recoveries were of 90.15+/-6.9% (C.V.'s<8%) for gemfibrozil and 93.10% for ibuprofen Applicability of the method was demonstrated by a pharmacokinetic study in normal volunteers who received gemfibrozil by oral route.
González-Peñas
E
E
Departamento de Química Orgánica y Farmacéutica, Facultad de Farmacia, Universidad de Navarra, 31008 Pamplona, Spain. mgpenas@unav.es
Agarraberes
S
S
López-Ocariz
A
A
García-Quetglas
E
E
Campanero
M A
MA
Carballal
J J
JJ
Honorato
J
J
D019540:Area Under Curve; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D015248:Gemfibrozil / Q000097:blood* / Q000493:pharmacokinetics; D006801:Humans; D000960:Hypolipidemic Agents / Q000097:blood* / Q000493:pharmacokinetics
D000960:Hypolipidemic Agents; D015248:Gemfibrozil