A new dynamin-like protein, ADL6, is involved in trafficking from the trans-Golgi network to the central vacuole in Arabidopsis.
Dynamin, a high-molecular-weight GTPase, plays a critical role in vesicle formation at the plasma membrane during endocytosis in animal cells. Here we report the identification of a new dynamin homolog in Arabidopsis named Arabidopsis dynamin-like 6 (ADL6). ADL6 is quite similar to dynamin I in its structural organization: a conserved GTPase domain at the N terminus, a pleckstrin homology domain at the center, and a Pro-rich motif at the C terminus. In the cell, a majority of ADL6 is associated with membranes. Immunohistochemistry and in vivo targeting experiments revealed that ADL6 is localized to the Golgi apparatus. Expression of the dominant negative mutant ADL6[K51E] in Arabidopsis protoplasts inhibited trafficking of cargo proteins destined for the lytic vacuole and caused them to accumulate at the trans-Golgi network. In contrast, expression of ADL6[K51E] did not affect trafficking of a cargo protein, H(+)-ATPase:green fluorescent protein, destined for the plasma membrane. These results suggest that ADL6 is involved in vesicle formation for vacuolar trafficking at the trans-Golgi network but not for trafficking to the plasma membrane in plant cells.
Jin
J B
JB
Department of Molecular Biology, Gyeongsang National University, Chinju 660-701, Korea.
Kim
Y A
YA
Kim
S J
SJ
Lee
S H
SH
Kim
D H
DH
Cheong
G W
GW
Hwang
I
I
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D029681:Arabidopsis Proteins; D002462:Cell Membrane / Q000378:metabolism; D034284:Dynamin I; D034281:Dynamins; D020558:GTP Phosphohydrolases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D007150:Immunohistochemistry; D008840:Microfilament Proteins / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D011487:Protein Conformation; D021381:Protein Transport; D006180:Proton-Translocating ATPases / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000032:analysis; D017421:Sequence Analysis; D014617:Vacuoles / Q000378:metabolism* / Q000648:ultrastructure; D021601:trans-Golgi Network / Q000201:enzymology* / Q000648:ultrastructure
D029681:Arabidopsis Proteins; D008840:Microfilament Proteins; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; D034284:Dynamin I; C434467:ADL6 protein, Arabidopsis; D020558:GTP Phosphohydrolases; D006180:Proton-Translocating ATPases; D034281:Dynamins