title: Analysis of seawaters for the recovery of culturable Vibrio parahaemolyticus and some other vibrios.
abstract: We investigated the recovery of dormant and injured cells along with the normally culturable cells of Vibrio species with special emphasis on V. parahaemolyticus using both selective and non-selective media at moderate (20 C) and standard (37 C) culture temperatures from a bay water environment. Culture temperatures (20 or 37 C) did not affect the recovery of V. parahaemolyticus but did for other vibrios. We observed similar seasonality of V parahaemolyticus as in most other environmental studies. V. parahaemolyticus and other Vibrio species were recovered in higher numbers by a replica plating method compared to most probable number (MPN) and direct TCBS (thiosulfate citrate bile-salt sucrose) agar counts. Even with the replica plating method, however, vibrios number goes down to a minimum level and V. parahaemolyticus was undetectable during the cool temperature period of the year, although total bacterial cells and CFU on nutrient agar (with 2% NaCl) did not vary so much during the study period.
authors.affiliation: Department of Environmental Hygiene, Faculty of Pharmaceutical Sciences, Okayama University, Okayama, Japan. d11008@pheasant.pharm.okayama-u.ac.jp
authors.forename: M J
authors.initials: MJ
authors.lastname: Alam
authors.forename: K
authors.initials: K
authors.lastname: Tomochika
authors.forename: S
authors.initials: S
authors.lastname: Miyoshi
authors.forename: S
authors.initials: S
authors.lastname: Shinoda
mesh_terms: D015169:Colony Count, Microbial; D003470:Culture Media; D017747:Seafood / Q000382:microbiology; D012623:Seawater; D014733:Vibrio / Q000254:growth & development / Q000302:isolation & purification*; D014736:Vibrio parahaemolyticus / Q000254:growth & development / Q000302:isolation & purification*
chemical_list: D003470:Culture Media