HIV gp120 and morphine alter mu opiate receptor expression in human vascular endothelium.
We find that chronic exposure of human saphenous vein, atria and internal thoracic artery endothelium to the human immunodeficiency virus surface glycoprotein gp120, results in an increase in endothelial mu opioid receptor expression (52%). gp120 acts, in this regard, as a proinflammatory cytokine (e.g. interleukin-1-alpha) by increasing endothelial mu opioid receptor expression. In contrast, morphine decreases mu opioid receptor expression by 90% in a dose dependent fashion. Pretreatment of these tissues with the respective antagonists e.g., naloxone and anti-gp120 blocks the opiate decrease and increase gp120 induced increase in mu expression, respectively. Further, pretreatment of these endothelia with morphine inhibits gp120-stimulated mu transcript expression. Therefore, the immune down-regulating action of morphine may prevent viral replication because this process requires immune activation that can, in part, be provided for by gp120 proinflammatory actions.
Cadet
P
P
Neuroscience Research Institute, State University of New York, College at Old Westbury, Old Westbury, NY 11568, USA.
Weeks
B S
BS
Bilfinger
T V
TV
Mantione
K J
KJ
Casares
F
F
Stefano
G B
GB
D000701:Analgesics, Opioid / Q000276:immunology* / Q000494:pharmacology; D046508:Culture Techniques; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects*; D015699:HIV Envelope Protein gp120 / Q000276:immunology* / Q000494:pharmacology; D006325:Heart Atria / Q000187:drug effects; D006801:Humans; D008323:Mammary Arteries / Q000187:drug effects; D009020:Morphine / Q000276:immunology* / Q000494:pharmacology; D012333:RNA, Messenger; D017450:Receptors, Opioid, mu / Q000235:genetics*; D012501:Saphenous Vein / Q000187:drug effects
D000701:Analgesics, Opioid; D015699:HIV Envelope Protein gp120; D012333:RNA, Messenger; D017450:Receptors, Opioid, mu; D009020:Morphine