title: DrhoGEF3 encodes a new Drosophila DH domain protein that exhibits a highly dynamic embryonic expression pattern.
abstract: The Rho GTPases regulate many different cellular and developmental processes, and activation of Rho GTPase signalling is mediated through interaction with the Dbl homology (DH) protein domain. We describe the expression pattern of DrhoGEF3 (cytological position 61B1-B3), which encodes a new member of the DH domain protein family from Drosophila and is a homologue of the human protein hPEM-2. During gastrulation and germ band extension, DrhoGEF3 exhibits a segmented expression pattern. DrhoGEF3 is subsequently expressed in the visceral mesoderm, at the sites of muscle attachment and in specific groups of sub-epidermal cells. The possible function of such a dynamically expressed signalling molecule is discussed.
authors.affiliation: Wellcome Trust Center for Cell-Matrix Research, School of Biological Sciences, University of Manchester, 2.205 Stopford Building, Oxford Road, Manchester, M13 9PT, UK.
authors.forename: M S
authors.initials: MS
authors.lastname: Hicks
authors.forename: V
authors.initials: V
authors.lastname: O'Leary
authors.forename: M
authors.initials: M
authors.lastname: Wilkin
authors.forename: S E
authors.initials: SE
authors.lastname: Bee
authors.forename: M J
authors.initials: MJ
authors.lastname: Humphries
authors.forename: M
authors.initials: M
authors.lastname: Baron
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004330:Drosophila / Q000196:embryology* / Q000235:genetics; D029721:Drosophila Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D020662:Guanine Nucleotide Exchange Factors / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D019476:Insect Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction; D020741:rho GTP-Binding Proteins / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D029721:Drosophila Proteins; D020662:Guanine Nucleotide Exchange Factors; D019476:Insect Proteins; C438831:rhoGEF3 protein, Drosophila; D020741:rho GTP-Binding Proteins