title: Topical application of brain derived neurotrophic factor influences upregulation of constitutive isoform of heme oxygenase in the spinal cord following trauma an experimental study using immunohistochemistry in the rat.
abstract: Influence of brain derived neurotrophic factor (BDNF) on carbon monoxide (CO) production following spinal cord injury was examined using expression of the constitutive isoform of the enzyme hemeoxygenase-2 (HO-2) in a rat model. A longitudinal incision of the right dorsal horn on the T10-11 segment markedly increased the HO-2 immunostaining in the cord at 5 h. At this time period, breakdown of the blood-spinal cord barrier (BSCB) and edema formation were quite prominent. Repeated topical application of BDNF (20 microliters of a 1 microgram/ml solution) over the exposed surface of the cord significantly attenuated the edematous expansion of the cord and the disturbances in the BSCB permeability. In BDNF-treated rats, expression of HO-2 immunoreactivity was considerably reduced. These results strongly suggest that BDNF is neuroprotective in spinal trauma and this growth factor has the capacity to attenuate CO production by downregulating HO-2 expression.
authors.affiliation: Laboratory of Neuroanatomy, Department of Medical Cell Biology, Biomedical Centre, Uppsala University, Uppsala, Sweden.
authors.forename: H S
authors.initials: HS
authors.lastname: Sharma
authors.forename: J
authors.initials: J
authors.lastname: Westman
authors.forename: T
authors.initials: T
authors.lastname: Gordh
authors.forename: P
authors.initials: P
authors.lastname: Alm
mesh_terms: D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000494:pharmacology*; D002248:Carbon Monoxide / Q000378:metabolism; D004487:Edema / Q000473:pathology*; D006419:Heme Oxygenase (Decyclizing) / Q000378:metabolism*; D007527:Isoenzymes / Q000378:metabolism*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013119:Spinal Cord Injuries / Q000473:pathology*; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D007527:Isoenzymes; D002248:Carbon Monoxide; D006419:Heme Oxygenase (Decyclizing); C105245:heme oxygenase-2