Toluene mineralization and growth potential of Pseudomonas putida PaW164 under toluene-limiting conditions.
Toluene-induced cells of Pseudomonas putida PaW164 (pWWO-164) were monitored for growth potential, maintaining the TOL plasmid, and potential toluene mineralization activity in toluene-amended and nonamended soil. A follow-up study was done in a carbon-free mineral salts solution to obtain further information on physiological changes that occur during starvation. These studies showed that there was a larger decline in colony forming units (CFUs) recovered on a toluate- or benzoate-defined mineral salts medium than on a complex agar medium, a greater percent decrease of CFU than of potential mineralization activity, no decrease in direct counts, and no loss of the TOL plasmid during starvation. Toluene-induced cells also showed an increasing lag time and a decreasing potential for mineralization of (14)C-toluene with starvation. In contrast, the lag time for mineralization of glucose was longest at the onset of starvation and reached a minimum by 3 days; thereafter, the potential for glucose mineralization remained high.
Lim
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TG
Department of Microbiology and Molecular Medicine, 124 Long Hall, Clemson University, Clemson, South Carolina 29634-0237, USA.
Gan
K D
KD
Hughes
T A
TA
Hayasaka
S S
SS
D002455:Cell Division; D002470:Cell Survival; D005947:Glucose / Q000378:metabolism; D008903:Minerals; D010957:Plasmids; D011549:Pseudomonas / Q000187:drug effects* / Q000254:growth & development*; D012989:Soil Pollutants / Q000009:adverse effects* / Q000378:metabolism; D013217:Starvation; D014050:Toluene / Q000009:adverse effects* / Q000378:metabolism
D008903:Minerals; D012989:Soil Pollutants; D014050:Toluene; D005947:Glucose