title: Antifungal activity of three spermidine conjugates.
abstract: Three tri-substituted spermidines, di-p-coumaroyl-caffeoylspermidine, tri-caffeoylspermidine and tri-p-coumaroylspermidine, isolated from pollen of Quercus alba, were examined for antifungal activity. Both di-p-coumaroyl-caffeoylspermidine and tri-p-coumaroylspermidine reduced mycelial growth of the oat leaf stripe pathogen, Pyrenophora avenae and reduced powdery mildew (Blumeria graminis f. sp. hordei) infection of barley seedlings when applied as a post-inoculation treatment. When used as a pre-inoculation treatment, only di-p-coumaroyl-caffeoylspermidine reduced powdery mildew infection significantly. Growth of P. avenae in the presence of 100 microM di-p-coumaroyl-caffeoylspermidine reduced activity of S-adenosylmethionine decarboxylase (AdoMetDC), and led to a reduction in the incorporation of labelled ornithine into spermidine. The other two spermidine conjugates increased AdoMetDC activity and the flux label from ornithine into spermine in P. avenae significantly.
authors.affiliation: Department of Plant Biology, The Scottish Agricultural College, Auchincruive, Nr Ayr, UK. d.walters@au.sac.ac.uk
authors.forename: D
authors.initials: D
authors.lastname: Walters
authors.forename: B
authors.initials: B
authors.lastname: Meurer-Grimes
authors.forename: I
authors.initials: I
authors.lastname: Rovira
mesh_terms: D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001203:Ascomycota / Q000187:drug effects* / Q000378:metabolism; D018554:Avena / Q000382:microbiology; D003470:Culture Media; D005516:Food Microbiology; D001467:Hordeum / Q000382:microbiology; D009952:Ornithine / Q000378:metabolism; D011058:Pollen / Q000737:chemistry; D011700:Putrescine / Q000378:metabolism; D013095:Spermidine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D013096:Spermine / Q000378:metabolism
chemical_list: D000935:Antifungal Agents; D003470:Culture Media; D013096:Spermine; D009952:Ornithine; D013095:Spermidine; D011700:Putrescine