title: [Removal of the 3'- and 5'-untranslated region amplifies expression of the erythropoietin gene in mammalian cells].
abstract: The effect of the 5T- and 3T-untranslated regions (UTR) of human erythropoietin gene (gEpo) on its expression in mammalian cells was investigated. 5T-UTR-deleted, 3T-UTR-deleted, and 5T,3T-UTR-deleted gEpos were transfected into COS-7 cells, and the amount of transiently expressed Epo was measured. Cells transfected with the 5T,3T-UTR-deleted gEpo showed the highest expression, 2.6 times that with wild-type gEpo. Northern blot analysis indicated that the enhanced expression of 5T,3T-UTR-deleted gEpo might be related with the transcription efficiency.
authors.forename: D Kh
authors.initials: DKh
authors.lastname: Park
authors.forename: C
authors.initials: C
authors.lastname: Kim
authors.forename: W B
authors.initials: WB
authors.lastname: Kim
authors.forename: Y K
authors.initials: YK
authors.lastname: Kim
authors.forename: S Y
authors.initials: SY
authors.lastname: Lee
authors.forename: J M
authors.initials: JM
authors.lastname: Yang
mesh_terms: D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D000818:Animals; D001483:Base Sequence; D019556:COS Cells; D017931:DNA Primers; D018076:DNA, Complementary; D004921:Erythropoietin / Q000235:genetics*; D005786:Gene Expression Regulation / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D017931:DNA Primers; D018076:DNA, Complementary; D012333:RNA, Messenger; D004921:Erythropoietin