Metabotropic neurosteroid/sigma-receptor involved in stimulation of nociceptor endings of mice.
In peripheral nociceptive flexor test, SA4503, (+)-pentazocine, and (+)-3-(hydroxyphenyl)-N-(1-propyl)piperidine, representative sigma-receptor agonists, elicited dose-dependent flexor responses. These responses were blocked by sigma-receptor antagonists NE-100 or BD1063, but not by pretreatments with antisense oligodeoxynucleotide for sigma1 binding protein. The sigma-agonists' nociception is attributed to the substance P (SP) release from nociceptor endings through activations of Galpha(i1) and phospholipase C (PLC). On the other hand, attomolar doses of neurosteroids such as dehydroepiandrosterone sulfate (DHEAS) and pregnenolone sulfate caused similar nociception, and they were blocked by progesterone (PROG). However, DHEAS nociception was not affected by pertussis toxin, but was completely inhibited by a PLC inhibitor or thapsigargin. Although the nociception by lower doses of DHEAS was abolished by diphenhydramine (DPH), H1 antagonist, there were dose-dependent responses by high doses of DHEAS in the presence of DPH. The responses by DHEAS in the presence of DPH were blocked by NE-100, and those by (+)-pentazocine were blocked by PROG. All these findings suggest that two novel types of neurosteroid receptors exist, neuronal NS1/sigma-type, which mediates activation of Galpha(i1) by neurosteroids and sigma-agonists, followed by SP release from nociceptor endings; and NS2 type, which mediates histamine release from mast cells by very low doses of neurosteroids.
Ueda
H
H
Department of Molecular Pharmacology and Neuroscience, Nagasaki University School of Pharmaceutical Sciences, Nagasaki, Japan. ueda@net.nagasaki-u.ac.jp
Inoue
M
M
Yoshida
A
A
Mizuno
K
K
Yamamoto
H
H
Maruo
J
J
Matsuno
K
K
Mita
S
S
D000818:Animals; D019314:Dehydroepiandrosterone Sulfate / Q000494:pharmacology; D004155:Diphenhydramine / Q000494:pharmacology; D005727:Ganglia, Spinal / Q000378:metabolism; D006634:Histamine H1 Antagonists / Q000494:pharmacology; D015151:Immunoblotting; D008297:Male; D051379:Mice; D009292:Narcotic Antagonists / Q000494:pharmacology; D009411:Nerve Endings / Q000187:drug effects / Q000502:physiology; D018377:Neurotransmitter Agents / Q000378:metabolism*; D009619:Nociceptors / Q000187:drug effects / Q000502:physiology*; D017933:Peripheral Nervous System / Q000378:metabolism; D037342:Pertussis Toxin; D017480:Receptors, sigma / Q000819:agonists / Q000037:antagonists & inhibitors / Q000378:metabolism*; D013256:Steroids / Q000378:metabolism*; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
D006634:Histamine H1 Antagonists; D009292:Narcotic Antagonists; D018377:Neurotransmitter Agents; D017480:Receptors, sigma; D013256:Steroids; D010566:Virulence Factors, Bordetella; D019314:Dehydroepiandrosterone Sulfate; D004155:Diphenhydramine; D037342:Pertussis Toxin