New evidence for the synteny of rice chromosome 1 and barley chromosome 3H from rice expressed sequence tags.
To provide improved access to the wealth of resources and genomic information that is presently being developed for rice a set of 88 rice expressed sequence tags (ESTs) previously mapped on rice chromosome I in the cross 'Nipponbare' x 'Kasalath' was used for comparative mapping in a cross of the barley cultivars 'Igri' and 'Franka'. As expected. most (89%) of the clones gave distinct banding patterns in barley of which about one-third was polymorphic between 'Igri' and 'Franka'. These polymorphisms were mapped, and most of these (56%) confirmed that rice chromosome 1 and barley chromosome 3H are syntenous. All single-copy markers identified conserved collinear positions, while markers with multiple copies did so in a few cases only. The markers that were not fitting in the collinear order were distributed randomly across the barley genome. The comparative maps of barley chromosome 3H and rice chromosome 1 comprise in total 26 common markers covering more than 95% of the genetic length of both chromosomes. A 30-fold reduction of recombination is seen around the barley centromere, and synteny may be interrupted in this region. However, the good overall synteny on a mesoscale (1-10 cM) justifies the use of rice as a platform for map-based cloning in barley.
Smilde
W D
WD
Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, Germany.
Haluskova
J
J
Sasaki
T
T
Graner
A
A
D002874:Chromosome Mapping; D002875:Chromosomes / Q000235:genetics*; D003001:Cloning, Molecular; D017124:Conserved Sequence; D003433:Crosses, Genetic; D015342:DNA Probes; D020224:Expressed Sequence Tags; D023061:Gene Order / Q000235:genetics*; D005819:Genetic Markers; D001467:Hordeum / Q000235:genetics*; D006824:Hybridization, Genetic; D012275:Oryza / Q000235:genetics*; D011110:Polymorphism, Genetic / Q000235:genetics; D016415:Sequence Alignment; D026801:Synteny / Q000235:genetics*
D015342:DNA Probes; D005819:Genetic Markers