Genetic diversity of Bacillus thuringiensis serovars revealed by RFLP using random DNA probes.
EcoRI and HindIII restriction fragment length polymorphism (RFLP) profiles using 2 random DNA probes, named 104 and 106, were generated for 85 B. thuringiensis strains. These include 80 serovars, 4 intra-serovar strains: kurstaki HD-1, dendrolimus, tenebrionis and sandiego, and a non-serotypeable strain B. thuringiensis var. wuhanensis. A total of 47 EcoRI and 65 HindIII restriction patterns were generated when hybridization results from both probes were combined. Seventy-seven B. thuringiensis strains showed distinctive hybridization profiles. The dendrogram resulting from the numerical analysis of the distance matrix revealed fourteen distinct phylogenetic groups at the 96% banding patterns similarity. The intra-serovar strains showed higher similarity with their respective type serovars. However, different serovars from a common H-serotype did not always cluster in the same phylogenetic group. Alternatively, several mosquitocidal serovars clustered in a single phylogenetic group. The correlation between serotyping and banding pattern similarity is discussed.
Joung
K B
KB
Programme de Doctorat en Sciences de l'environnement, Université du Québec à Montréal, Montréal, Québec, Canada.
Lemaire
S P
SP
Côté
J C
JC
D001413:Bacillus thuringiensis / Q000145:classification* / Q000235:genetics*; D015373:Bacterial Typing Techniques; D003001:Cloning, Molecular; D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis; D015246:Deoxyribonuclease EcoRI / Q000378:metabolism; D015247:Deoxyribonuclease HindIII / Q000378:metabolism; D014644:Genetic Variation; D009693:Nucleic Acid Hybridization / Q000379:methods; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D012703:Serotyping
D015342:DNA Probes; D004269:DNA, Bacterial; D015246:Deoxyribonuclease EcoRI; D015247:Deoxyribonuclease HindIII