title: Adenine toxicity and transport in the moderately halophilic eubacterium Halomonas elongata.
abstract: The presence of adenine in the L-alanine defined medium substantially inhibited the growth of the moderately halophilic eubacterium Halomonas elongata. Extensive attempts to reverse the adenine toxicity for growth were made using a variety of purine and pyrimidine compounds, vitamins, and amino acids. Of the compounds tested, only cytosine was found to reverse the adenine growth inhibition. This indicates a mechanism similar to that found for some strains of Escherichia coli in which the presence of exogenous purines (e.g. adenine) was found to stop purine de novo synthesis and repress the synthesis of the pyrimidine salvage enzyme cytosine deaminase. H. elongata was found to possess an active adenine uptake system that was sodium dependent with only lithium having a considerable capacity to replace the sodium. A competition study indicated that the adenine transport system was quite specific. This paper represents the initial study of purine and pyrimidine salvage pathways and adenine uptake for the moderately halophilic eubacteria.
authors.affiliation: School of Biological Sciences, University of Nebraska, Lincoln, NE 68588, USA.
authors.forename: A M
authors.initials: AM
authors.lastname: Perlman
authors.forename: J K
authors.initials: JK
authors.lastname: Wolfe
authors.forename: T E
authors.initials: TE
authors.lastname: Bush
authors.forename: J K
authors.initials: JK
authors.lastname: Dyer
authors.forename: E L
authors.initials: EL
authors.lastname: Martin
mesh_terms: D000225:Adenine / Q000378:metabolism* / Q000494:pharmacology*; D001693:Biological Transport, Active; D003470:Culture Media; D003596:Cytosine / Q000378:metabolism; D020632:Halomonas / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism*; D011687:Purines / Q000378:metabolism; D011743:Pyrimidines / Q000378:metabolism; D012965:Sodium Chloride
chemical_list: D003470:Culture Media; D011687:Purines; D011743:Pyrimidines; D012965:Sodium Chloride; D003596:Cytosine; D000225:Adenine