title: Suppression of melanoma cell proliferation by histidine decarboxylase specific antisense oligonucleotides.
abstract: Histidine decarboxylase (HDC) is expressed by the cells of melanoma, in which the histamine content tends to be relatively high. This study shows that elevated expression of HDC was found by western blot analysis of primary and metastatic melanoma tissue using a polyclonal HDC specific antibody. The specificity of anti-HDC antibody was confirmed by inhibition of HDC translation (i.e., immunopositivity) in melanoma cells by HDC-specific antisense oligonucleotide. Moreover, the decrease in proliferation caused by HDC antisense oligonucleotides indicates considerable functional relevance of histamine synthesis in melanoma growth and suggests a possible in situ application of specific antisense oligonucleotides for HDC in melanoma therapy.
authors.affiliation: Department of Genetics, Cell and Immunobiology, Semmelweis University, Budapest, Hungary.
authors.forename: H
authors.initials: H
authors.lastname: Hegyesi
authors.forename: B
authors.initials: B
authors.lastname: Somlai
authors.forename: V L
authors.initials: VL
authors.lastname: Varga
authors.forename: G
authors.initials: G
authors.lastname: Toth
authors.forename: P
authors.initials: P
authors.lastname: Kovacs
authors.forename: E L
authors.initials: EL
authors.lastname: Molnar
authors.forename: V
authors.initials: V
authors.lastname: Laszlo
authors.forename: S
authors.initials: S
authors.lastname: Karpati
authors.forename: E
authors.initials: E
authors.lastname: Rivera
authors.forename: A
authors.initials: A
authors.lastname: Falus
authors.forename: Z
authors.initials: Z
authors.lastname: Darvas
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001706:Biopsy; D002455:Cell Division / Q000235:genetics; D005260:Female; D015316:Genetic Therapy; D006640:Histidine Decarboxylase / Q000235:genetics*; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008545:Melanoma / Q000378:metabolism / Q000473:pathology* / Q000628:therapy; D008875:Middle Aged; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D012878:Skin Neoplasms / Q000378:metabolism / Q000473:pathology* / Q000628:therapy; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000201:enzymology
chemical_list: D016376:Oligonucleotides, Antisense; D006640:Histidine Decarboxylase