[Importance of Helicobacter pylori in the pathogenesis of gastric cancer. Experimental models in rodents].
We found that the seroprevalence in Cancer Institute of H. pylori infection was significantly more frequent in gastric cancer than in age- and gender-matched controls. This study suggested an epidemiological link between H. pylori infection and gastric cancer. H. pylori exhibits a complex system of enzymes which serve a range of functions. Toxic effects are produced by urease (UR), phospholipase (PL) and alcohol dehydrogenase (ADH). We embarked on an exploration of the enzyme activities of H. pylori infected patients using a TLC-autoradioluminography. This method has a wide dynamic range and could offer an analytical technique for studying a radioactive compound and its enzymes in H. pylori infected mucosa. Biopsies samples taken from 21 gastric cancer patients and 95 controls were studied. Although high activity of UR indicates well the presence of H. pylori impairment, activities of ADH and PL reflects more the chronicity of mucosal damage in both groups. Clearly, the enzyme profile showed in our study reflects the "physiological" adaptations behind chronic injured mucosal changes but its relation to gastric cancer and H. pylori needs further study. There is an urgent need to understand the carcinogenesis process using animal models. We performed previous study for to explore the effect of H. pylori infection on N- methyl-N-nitrosourea-induced (MNU) gastric carcinogenesis in mice C57BL/6 mice were administered broth culture of H. pylori and given MNU in drinking water. In terms of the incidence of neoplasms development was increase in the MNU group pre-infected with H. pylori. That findings showed that C57BL/6 mice-infected model is well suited for investigating the bacteria promoter effect in the gastric carcinogenesis. Finally another rodent model study (still in process) showed rapid development of hyperplastic gastritis with gastric erosions in H. pylori-infected MTH1 knockout mice. We sought to further evaluate MTH1 knockout mice as potential test animal for carcinogenesis.


CONCLUSIONS
It is suggested that H. pylori infection is an important risk factor for the development of gastric cancer. The possibility that this organism acts etiologically, exerting its effect over long period of time, is biologically plausible. However, the role of H. pylori per se in that process is still a matter of discussion. The various enzymes of H. pylori discussed in this paper support colonization, and are perhaps important for epithelial damage, they could contribute to the stimulation and modulation of the chronic inflammatory response, but its relation to gastric cancer and H. pylori needs further study. Finally H. pylori in C57BL/6 and knockout mice showed excellent colonization at two months and six months after infection there was adenomatous, hyperplastic and ulcerative changes. Those findings showed that both mice-infected models are well suited for investigating the bacteria promoter effect in the gastric carcinogenesis.
Barreto-Zúñiga
R
R
Servicio de Gastroenterología y Endoscopia, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Vasco de Quiroga 15, Sección XVI, México, D.F.
Kato
Y
Y
Bobadilla
D J
DJ
Okuyama
M
M
Maruyama
M
M
Ohta
H
H
Takekoshi
T
T
Shigematsu
A
A
D000230:Adenocarcinoma / Q000139:chemically induced / Q000209:etiology* / Q000382:microbiology; D000426:Alcohol Dehydrogenase / Q000502:physiology; D000818:Animals; D001426:Bacterial Proteins / Q000502:physiology; D002855:Chromatography, Thin Layer; D002908:Chronic Disease; D003043:Cocarcinogenesis; D004195:Disease Models, Animal; D005260:Female; D005753:Gastric Mucosa / Q000382:microbiology / Q000473:pathology; D005756:Gastritis / Q000209:etiology / Q000382:microbiology; D016480:Helicobacter pylori / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D006801:Humans; D006965:Hyperplasia; D015994:Incidence; D008297:Male; D008770:Methylnitrosourea / Q000633:toxicity; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D010740:Phospholipases / Q000502:physiology; D013274:Stomach Neoplasms / Q000139:chemically induced / Q000209:etiology* / Q000382:microbiology; D013276:Stomach Ulcer / Q000209:etiology / Q000382:microbiology; D014510:Urease / Q000502:physiology; D014774:Virulence
D001426:Bacterial Proteins; D008770:Methylnitrosourea; D000426:Alcohol Dehydrogenase; D010740:Phospholipases; D014510:Urease