On-line determination of animal cell concentration.
A new approach for the indirect determination of cell concentration in the case of nonconstant metabolic rates has been developed. The specific glucose-uptake rate was shown to be nonconstant in batch cultures of free suspended and immobilized CHO SSF3 cells. Time-independent models correlating the specific rate to the limiting substrate concentration were established, thus providing a continuous determination of the specific rate through on-line measurement of the limiting substrate. The method could be applied to determine on-line cell concentration in both free suspended and immobilized cell cultures. Results were verified off-line by crystal violet nuclei counting. The predicted cell concentration was in very good agreement with the off-line reference during the whole exponential-growth phase, until the specific glucose-uptake rate tended to zero.
Ducommun
P
P
Institute of Chemical Engineering, Swiss Federal Institute of Technology (EPFL), CH-1015 Lausanne, Switzerland.
Bolzonella
I
I
Rhiel
M
M
Pugeaud
P
P
von Stockar
U
U
Marison
I W
IW
D018533:Biomass; D002138:Calibration; D002452:Cell Count / Q000379:methods*; D002460:Cell Line; D002477:Cells / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006801:Humans; D012016:Reference Values
D005947:Glucose