title: Analysis of polymorphic regions of Plasmodium vivax Duffy binding protein of Korean isolates.
abstract: The present study was designed to investigate polymorphism in Duffy binding protein (DBP) gene of Plasmodium vivax isolates of Korea. Thirty samples were obtained from P. vivax patients in Yonchon-gun, Kyonggi-do in 1998. The PCR products of the samples were subjected to sequencing and hybridization analyses of the regions II and IV of P. vivax DBP gene. Two genotypes, SK-1 and SK-2, were identified on the basis of amino acid substitution and deletion. The genotype of 10 isolates was SK-1 and that of 20 isolates was SK-2. Most of the predicted amino acids in the region II of DBP gene were conserved between the Korean isolates and Belem strain except for 4-5 amino acid substitutions. In the region IV of DBP, a 6-bp insert that was shown in the Sal-1 allele type was found in SK-1, and a 27-bp insert that was shown in the Papua New Guinea allele type was found in SK-2. In conclusion, the present findings suggest that two genotypes of P. vivax coexist in the endemic area of Korea.
authors.affiliation: Department of Parasitology, Institute of Malariology, Inje University College of Medicine, Pusan 614-735, Korea. wgkho@ijnc.inje.ac.kr
authors.forename: W G
authors.initials: WG
authors.lastname: Kho
authors.forename: J Y
authors.initials: JY
authors.lastname: Chung
authors.forename: E J
authors.initials: EJ
authors.lastname: Sim
authors.forename: D W
authors.initials: DW
authors.lastname: Kim
authors.forename: W C
authors.initials: WC
authors.lastname: Chung
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000953:Antigens, Protozoan; D001483:Base Sequence; D002352:Carrier Proteins / Q000032:analysis* / Q000737:chemistry / Q000235:genetics*; D016054:DNA, Protozoan / Q000235:genetics; D005838:Genotype; D006801:Humans; D007723:Korea; D016780:Malaria, Vivax / Q000469:parasitology; D008969:Molecular Sequence Data; D010966:Plasmodium vivax / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D015800:Protozoan Proteins; D011956:Receptors, Cell Surface / Q000032:analysis* / Q000737:chemistry / Q000235:genetics*
chemical_list: D000953:Antigens, Protozoan; D002352:Carrier Proteins; D016054:DNA, Protozoan; C066001:Duffy antigen binding protein, Plasmodium; D015800:Protozoan Proteins; D011956:Receptors, Cell Surface