The effect of heat shock on amino acid transport and cell volume in 3T3 cells.
In 3T3 cells temperatures higher than physiological stimulated amino acid transport activity in a dose-dependent manner up to 44 degrees C. However, the temperature increase did not induce widespread transport increase of all other nutrients tested. The activities of both amino acid transport systems A and ASC were enhanced within a few minutes following cell exposure to increased temperature. The maintenance of this effect required continuous exposure of the cells to hyperthermia. Kinetic analysis indicated that the stimulation of the activity of transport System A occurred through a mechanism affecting Vmax rather than Km. The continuous presence of cycloheximide did not prevent the transport changes induced by hyperthermia. These results suggest that the increased amino acid uptake reflects an activation or relocation of existing amino acid transport proteins. During the hyperthermic treatment, the content of ninhydrin-positive substances (NPS), mostly amino acids, increased within the cells and the accumulation of these compatible osmolytes was parallelled by an increase in cell volume. The withdrawal of amino acids from the culture medium immediately before and during the shock phase counteracted the increase and reduced the NPS content but did not prevent the increase in amino acid transport, the cell swelling and the induction of the heat shock response.
Petronini
P G
PG
Dipartimento di Medicina Sperimentale, Università degli Studi di Parma, Italy.
Caccamo
A E
AE
Alfieri
R R
RR
Bonelli
M A
MA
Borghetti
A F
AF
D016475:3T3 Cells; D000596:Amino Acids / Q000378:metabolism / Q000493:pharmacokinetics; D000818:Animals; D001692:Biological Transport; D015152:Blotting, Northern; D003513:Cycloheximide / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005973:Glutamine / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D006358:Hot Temperature; D007700:Kinetics; D051379:Mice; D009555:Ninhydrin / Q000378:metabolism; D011392:Proline / Q000378:metabolism; D011485:Protein Binding; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D013312:Stress, Physiological; D013696:Temperature; D013997:Time Factors; D015091:beta-Alanine / Q000031:analogs & derivatives* / Q000493:pharmacokinetics
D000596:Amino Acids; D018840:HSP70 Heat-Shock Proteins; D011500:Protein Synthesis Inhibitors; D005973:Glutamine; D015091:beta-Alanine; C012924:2,2-dimethyl-beta-alanine; D003513:Cycloheximide; D011392:Proline; D009555:Ninhydrin