title: Ionic and non-ionic contrast agent-mediated endothelial injury. Quantitative analysis of cell proliferation during endothelial repair.
abstract: OBJECTIVE
  To quantitatively evaluate endothelial injury in vivo and to assess the time course of cellular repair after endothelial cell exposure to ionic and non-ionic contrast media.
  
  
  METHODS
  Local changes at the cellular level following intraaortic injection of 1 ml of the ionic contrast agent ioxithalamate or 1 ml of the non-ionic contrast agent iomeprol, each with an iodine content of 300 mg/ml, were investigated using rat endothelium as an in vivo model. A sorbitol solution iso-osmolar to iomeprol served as control. Quantitative analysis of endothelial changes by autoradiography of 3H-thymidine-labeled endothelial cells was assessed after 3, 5 and 10 days, determining the 3H-thymidine index and the DNA synthesis rate.
  
  
  RESULTS
  Ioxithalamate showed a clear harmful effect on the endothelium, with an elevated 3H-thymidine index of 7.68% on day 3 and 6.89% on day 5, versus 2.97% on day 3 and 2.55% on day 5 for iomeprol and 2.29% on day 3 and 1.91% on day 5 for the control.
  
  
  CONCLUSIONS
  Ionic contrast agents lead to reversible transient focal endothelial cell injury. No such side effect was detectable for non-ionic contrast agents.
authors.affiliation: Department of Diagnostic Radiology, University of Ulm, Ulm, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Gabelmann
authors.forename: J
authors.initials: J
authors.lastname: Haberstroh
authors.forename: G
authors.initials: G
authors.lastname: Weyrich
mesh_terms: D000818:Animals; D001011:Aorta / Q000473:pathology; D001345:Autoradiography; D002455:Cell Division / Q000187:drug effects; D003287:Contrast Media / Q000633:toxicity*; D004247:DNA / Q000096:biosynthesis; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D007479:Iopamidol / Q000031:analogs & derivatives* / Q000633:toxicity*; D007483:Iothalamic Acid / Q000031:analogs & derivatives / Q000633:toxicity*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013936:Thymidine / Q000378:metabolism
chemical_list: D003287:Contrast Media; D007483:Iothalamic Acid; C057937:iomeprol; D004247:DNA; C002587:ioxitalamic acid; D007479:Iopamidol; D013936:Thymidine