title: von Willebrand Factor A domain-related protein, a novel microneme protein of the malaria ookinete highly conserved throughout Plasmodium parasites.
abstract: The mosquito-invasive form of the malarial parasite, the ookinete, develops numerous secretory organelles, called micronemes, in the apical cytoplasm. Micronemal proteins are thought to be secreted during midgut invasion and to play a crucial role in attachment and motility of the ookinete. We found a novel ookinete micronemal protein of rodent malarial parasite Plasmodium berghei, named P. berghei von Willebrand factor A domain-related protein (PbWARP), and report it here as a putative soluble adhesive protein of the ookinete. The PbWARP gene contained a single open reading frame encoding a putative secretory protein of 303 amino acids, with a von Willebrand factor type A module-like domain as a main component. Western blot analysis demonstrated that PbWARP was firstly produced 12 h after fertilization by maturing ookinetes as SDS-resistant complexes. Recombinant PbWARP produced with a baculovirus system also formed SDS-resistant high-order oligomers. Immuno-electron microscopic studies showed that PbWARP was randomly distributed in the micronemes. PbWARP homologues also exist in human malarial parasites, Plasmodium falciparum and Plasmodium vivax. Highly conserved primary structures of PbWARP homologues among these phylogenetically distant Plasmodium species suggest their functional significance and the presence of a common invasion mechanism widely utilized throughout Plasmodium parasites.
authors.affiliation: Department of Medical Zoology, Mie University School of Medicine, Edobashi, Tsu, 514-0001, Japan. m-yuda@doc.medic.mie-u.ac.jp
authors.forename: M
authors.initials: M
authors.lastname: Yuda
authors.forename: K
authors.initials: K
authors.lastname: Yano
authors.forename: T
authors.initials: T
authors.lastname: Tsuboi
authors.forename: M
authors.initials: M
authors.lastname: Torii
authors.forename: Y
authors.initials: Y
authors.lastname: Chinzei
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002451:Cell Compartmentation; D003001:Cloning, Molecular; D017124:Conserved Sequence; D008969:Molecular Sequence Data; D015388:Organelles / Q000235:genetics*; D010961:Plasmodium; D010962:Plasmodium berghei / Q000235:genetics* / Q000472:pathogenicity; D011487:Protein Conformation; D015800:Protozoan Proteins / Q000235:genetics*; D017422:Sequence Analysis, DNA; D014841:von Willebrand Factor / Q000235:genetics
chemical_list: D015800:Protozoan Proteins; C434321:WARP protein, Plasmodium; D014841:von Willebrand Factor