Tuning DNA "strings": modulating the rate of DNA replication with mechanical tension.
Recent experiments have measured the rate of replication of DNA catalyzed by a single enzyme moving along a stretched template strand. The dependence on tension was interpreted as evidence that T7 and related DNA polymerases convert two (n = 2) or more single-stranded template bases to double helix geometry in the polymerization site during each catalytic cycle. However, we find structural data on the T7 enzyme--template complex indicate n = 1. We also present a model for the "tuning" of replication rate by mechanical tension. This model considers only local interactions in the neighborhood of the enzyme, unlike previous models that use stretching curves for the entire polymer chain. Our results, with n = 1, reconcile force-dependent replication rate studies with structural data on DNA polymerase complexes.
Goel
A
A
Department of Physics and Harvard-Massachusetts Institute of Technology Joint Division of Health Sciences and Technology, Harvard University, Cambridge, MA 02138, USA.
Frank-Kamenetskii
M D
MD
Ellenberger
T
T
Herschbach
D
D
D017123:Bacteriophage T7 / Q000235:genetics; D004247:DNA / Q000737:chemistry*; D004256:DNA Polymerase I / Q000737:chemistry*; D004261:DNA Replication; D004277:DNA, Single-Stranded / Q000737:chemistry*; D004279:DNA, Viral / Q000737:chemistry; D004259:DNA-Directed DNA Polymerase / Q000737:chemistry; D008958:Models, Molecular; D009690:Nucleic Acid Conformation
D004277:DNA, Single-Stranded; D004279:DNA, Viral; D004247:DNA; C034970:bacteriophage T7 induced DNA polymerase; D004256:DNA Polymerase I; D004259:DNA-Directed DNA Polymerase