GTP gamma S-induced Ca2+ activated Cl- currents: its stable induction by Gq alpha overexpression in Xenopus oocytes.
In native Xenopus oocytes, injection of guanosine 5'-O-(3-thiotriphosphate) (GTPgammaS) (30 mM, 5 nl) did not induce Cl- current in 11 out of 22 oocytes. Injection of increased concentration of GTPgammaS (100 mM, 5 nl) into the oocytes induced Cl- currents in 16 out of 17 oocytes; however, the size of the induced currents was extremely varied. In oocytes overexpressing Gq alpha, GTPgammaS (30 mM, 5 nl) faithfully evoked Ca2+-activated Cl- currents. These results indicate that heterogeneous expression of Gq alpha in Xenopus oocytes provides a useful system for studying the functional roles of Gq alpha in regulating cellular events.
Kaibara
M
M
Department of Pharmacology, Nagasaki University School of Medicine, Japan. mkaibara@alpha.med.nagasaki-u.ac.jp
Nagase
Y
Y
Murasaki
O
O
Uezono
Y
Y
Doi
Y
Y
Taniyama
K
K
D000818:Animals; D002118:Calcium / Q000502:physiology*; D002413:Cations, Divalent; D018118:Chloride Channels / Q000096:biosynthesis*; D005260:Female; D043802:GTP-Binding Protein alpha Subunits, Gq-G11; D016244:Guanosine 5'-O-(3-Thiotriphosphate) / Q000494:pharmacology*; D020962:Heterotrimeric GTP-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics*; D008845:Microinjections; D009865:Oocytes / Q000187:drug effects* / Q000378:metabolism*; D051381:Rats; D014981:Xenopus
D002413:Cations, Divalent; D018118:Chloride Channels; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D043802:GTP-Binding Protein alpha Subunits, Gq-G11; D020962:Heterotrimeric GTP-Binding Proteins; D002118:Calcium