title: Effects of a bioactive glass on healing of closed skin wounds in dogs.
abstract: OBJECTIVE
  To determine effects of intraincisional bioactive glass on healing of sutured skin wounds in dogs.
  
  
  METHODS
  9 purpose-bred mature female Beagles.
  
  
  METHODS
  3 small matched bilateral (treated vs control) full-thickness truncal skin incisions were made and sutured. Treated wounds received intraincisional particulate bioactive glass prior to closure. Laser Doppler perfusion imaging was used to assess percentage change in tissue perfusion 3 and 5 days after incision on 1 set of 2 matched wounds, and skin and subcutaneous tissue-cutaneous trunci breaking strength were assessed at 5 days. The other 2 sets of wounds were used for histologic evaluation at 5 and 21 days, respectively.
  
  
  RESULTS
  Subjective signs of gross inflammatory reaction were not detected in treated or control wounds. At 5 days, median subcutaneous tissue-cutaneous trunci breaking strength was significantly higher in treated wounds than in control wounds-(188.75 vs 75.00 g). At 5 days, median scores were significantly higher for neutrophils (1 vs 0), macrophages (2 vs 1), and necrosis (1 vs 0) for treated wounds than for control wounds. At 21 days, median macrophage scores were significantly higher for treated wounds than for control wounds (2 vs 1).
  
  
  CONCLUSIONS
  Bioactive glass in soft tissues does not cause a gross inflammatory reaction but causes an increase in histologic signs of inflammation, which decreases with time. Bioactive glass has potential for increasing tissue strength. Increased subcutaneous breaking strength could be beneficial in treating wounds in which early healing strength is needed.
authors.affiliation: Scott-Ritchey Research Center, College of Veterinary Medicine, Auburn University, AL 36849, USA.
authors.forename: R L
authors.initials: RL
authors.lastname: Gillette
authors.forename: S F
authors.initials: SF
authors.lastname: Swaim
authors.forename: E A
authors.initials: EA
authors.lastname: Sartin
authors.forename: D M
authors.initials: DM
authors.lastname: Bradley
authors.forename: S L
authors.initials: SL
authors.lastname: Coolman
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D003094:Collagen / Q000032:analysis / Q000096:biosynthesis; D062109:Dermatologic Surgical Procedures; D004285:Dogs / Q000502:physiology / Q000601:surgery*; D005260:Female; D005898:Glass; D006651:Histocytochemistry / Q000662:veterinary; D017078:Laser-Doppler Flowmetry / Q000662:veterinary; D012867:Skin / Q000293:injuries*; D013537:Sutures; D014945:Wound Healing / Q000187:drug effects*; D014947:Wounds and Injuries / Q000628:therapy / Q000662:veterinary
chemical_list: D001672:Biocompatible Materials; D003094:Collagen