Development of an improved automated gas-chromatographic chiral analysis system: application to non-natural amino acids and natural protein hydrolysates.
In the use of combinatorial chemistry as a novel strategy for drug discovery, the chirality assessment of building blocks used for library construction is particularly important in the evaluation of biological actions of generated libraries. The procedure for chiral analysis of lead compounds in screening may be of a high priority, particularly in the case of the protection of intellectual rights. Previously, an automated amino acid analysis system using enantiomer labeling was developed. The system incorporates a reactor, which allows automated esterification and acylation of amino acids, and is connected to an on-line gas chromatographic system. A capillary column coated with a chiral phase is employed for the separation of the enantiomers. This particular system is improved with a newly constructed "high-throughput auto-derivatizer" in combination with a new GC-system. The resulting data can be processed by newly constructed software. The analyses of amino acid derivatives or hydrolysates of proteins and peptides are carried out routinely within ca. 45 min, including derivatization. Using this system several non-natural amino acids were tested with respect to the stereoisomeric configuration. In addition, acid hydrolysates of food proteins and tissues obtained by autopsy were analyzed as an application to proteome research.
Nokihara
K
K
Shimadzu Scientific Research Inc., Nishinokyo-Kuwabaracho 1, Nakagyo-ku, Kyoto 604-8442, Japan. noki@shimadzu.co.jp
Gerhardt
J
J
D000368:Aged; D000369:Aged, 80 and over; D000596:Amino Acids / Q000032:analysis*; D001923:Brain Chemistry; D002849:Chromatography, Gas / Q000295:instrumentation / Q000379:methods*; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D006868:Hydrolysis; D008875:Middle Aged; D008894:Milk Proteins / Q000032:analysis; D009419:Nerve Tissue Proteins / Q000032:analysis; D011506:Proteins / Q000032:analysis*; D013237:Stereoisomerism
D000596:Amino Acids; D008894:Milk Proteins; D009419:Nerve Tissue Proteins; D011506:Proteins