title: Serotype and adhesion of Pseudomonas aeruginosa isolated from contact lens wearers.
abstract: The purpose of the present study was to correlate the serotypes of Pseudomonas aeruginosa to the bacterial adhesion to contact lenses and human corneal epithelial cells. Twenty-three strains isolated from contact lens wearers were used for the study. The bacterial serotypes were examined with a P. aeruginosa antisera kit. The attachment of bacteria on contact lenses or human corneal epithelial cells was determined by counting the number of adhered bacteria after incubation of the bacteria with contact lenses or corneal epithelial cells. The 23 ocular isolates belonged to seven serotypes. Strains of serotypes I, G and E were the three dominant serogroups and were more adhesive to contact lenses compared with other groups of the bacteria. The bacterial serotypes and the clinical sequelae were not strongly related. These results indicate that the surface characteristics of bacterial serotypes are related to the bacterial adhesion to the surface, but the pathogenesis of the bacteria may result from multiple factors.
authors.affiliation: Cooperative Research Centre for Eye Research and Technology, The University of New South Wales, Sydney, Australia. sthu@cclru.unsw.edu.au
authors.forename: S J
authors.initials: SJ
authors.lastname: Thuruthyil
authors.forename: H
authors.initials: H
authors.lastname: Zhu
authors.forename: M D
authors.initials: MD
authors.lastname: Willcox
mesh_terms: D001422:Bacterial Adhesion; D015169:Colony Count, Microbial; D003261:Contact Lenses / Q000382:microbiology*; D003315:Cornea / Q000166:cytology / Q000382:microbiology*; D004847:Epithelial Cells / Q000382:microbiology; D006801:Humans; D007634:Keratitis / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000145:classification* / Q000302:isolation & purification / Q000502:physiology*; D012703:Serotyping