Summary of workshop findings for porcine myelomonocytic markers.
About 65 monoclonal antibodies (mAb) including 17 internal controls were analyzed for their ability to recognize and bind to various cells of the myelomonocytic lineage. Flow cytometry (FCM) utilizing both single and double staining, and immunoprecipitation (IP) assays were used in the analysis. About 38 of the mAb were reactive with myelomonocytic cells, resulting in nine clusters of interest. Although the exact identity of many of the molecules on the cells bound by the mAb remains undetermined, information obtained about the mAb analyzed in this workshop should be helpful in further identifying various populations of myelomonocytic cells and their stages of differentiation. Out of 12 mAbs with potential CD11 specificity, seven were assigned to three different swine specific alpha chains of the CD11/CD18 integrin heterodimer, the assignment of the remaining four was tentative. One antibody had a binding specificity consistent with SWC3 and one with SWC8. CD14 expression on pig cells was characterized with a panel of CD14-positive antibodies, two of these antibodies were assigned to swine CD14. Two antibodies were assigned to CD163. Further work is required to determine the antigens recognized by many of the other mAb.
Thacker
E
E
Veterinary Medical Research Institute, Iowa State University, Ames, IA 50010, USA. ethacker@iastate.edu
Summerfield
A
A
McCullough
K
K
Ezquerra
A
A
Dominguez
J
J
Alonso
F
F
Lunney
J
J
Sinkora
J
J
Haverson
K
K
D000818:Animals; D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D016000:Cluster Analysis; D005434:Flow Cytometry; D018950:Lipopolysaccharide Receptors; D009000:Monocytes / Q000145:classification / Q000166:cytology / Q000276:immunology*; D013552:Swine / Q000276:immunology*
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D018950:Lipopolysaccharide Receptors