Detection of moderate hyperhomocysteinemia: comparison of the Abbott fluorescence polarization immunoassay with the Bio-Rad and SBD-F high-performance liquid chromatographic assays.
The importance of accurate methods for homocysteine measurement has been emphasized. We compared the results obtained with the most commonly used high-performance liquid chromatography (HPLC) assay, and two recently commercially available methods: another HPLC and a fluorescence polarization immunoassay, in plasmas from normo- or hyperhomocysteinemic patients. A significant agreement between the different methods in classifying the results as hyper or normal-homocysteinemia was observed. However, a significant difference between the results was found. Standardization is urgently necessary to improve the concordance of homocysteine determination.
Barbé
F
F
Laboratory of Biochemistry A, INSERM U308, Vandoeuvre-lès-Nancy, France. f.barbe@chu-nancy.fr
Abdelmouttaleb
I
I
Chango
A
A
Gérard
P
P
Quilliot
D
D
Klein
M
M
Lambert
D
D
Nicolas
J P
JP
Guéant
J L
JL
D002624:Chemistry, Clinical / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003545:Cysteine / Q000097:blood*; D003920:Diabetes Mellitus / Q000097:blood; D006331:Heart Diseases / Q000097:blood; D006801:Humans; D020138:Hyperhomocysteinemia / Q000175:diagnosis*; D006980:Hyperthyroidism / Q000097:blood; D007037:Hypothyroidism / Q000097:blood; D013050:Spectrometry, Fluorescence / Q000379:methods*
D003545:Cysteine