[Errors in measurement of section areas and perimeters of microvessels from their profiles in sections for light and electron microscopy].
The error of measuring the section areas and perimeters of microvessels was estimated, which is due to a deviation of the section plane from the direction perpendicular to the longitudinal axis of microvessels. The estimates of the microvessel nonperpendicular sectioning error correspond to the case of three-dimensional isotropic distribution of microvessels without their own eccentricity. Only those microvessel profiles are selected for morphometry that meet the condition theta min < or = b/c < or = 1.00, where b is the minor and c is the major radii of microvessel profile on a section, and theta min is the tolerance for profile nonperpendicularity.
Krasnoperov
R A
RA
Sechenov Moscow Medical Academy, ul. Bol'shaya Pirogovskaya 2-6, Moscow, 119881 Russia.
Grachev
S V
SV
Gerasimov
A N
AN
D000818:Animals; D001808:Blood Vessels / Q000033:anatomy & histology*; D001921:Brain / Q000098:blood supply; D003627:Data Interpretation, Statistical; D008833:Microcirculation / Q000033:anatomy & histology; D008853:Microscopy / Q000379:methods / Q000706:statistics & numerical data*; D008854:Microscopy, Electron / Q000379:methods / Q000706:statistics & numerical data*; D051381:Rats