Prosaposin-derived peptides enhanced sprouting of sensory neurons in vitro and induced sprouting at motor endplates in vivo.
Prosaposin exhibits neurotrophic factor properties that are localized to a 12-amino acid sequence located in the amino terminal portion of the saposin C domain. Prosaptides are peptides derived from the neurotrophic portion of prosaposin; these have been previously reported to be bioactive in neuroblastoma cell lines in vitro. We report that prosaptides were also bioactive in explants of adult primary sensory neurons by dose-dependently increasing both the number (3- to 4-fold) and elongation of these neurites by 50%. Local injection of prosaptides into the gluteus muscle of adult mice also induced sprouting at the motor endplate. Our results indicate that prosaptides are potent neuritogenic factors for both sensory and motor neurons of adult peripheral nerve.
Campana
W M
WM
Department of Neuroscience, University of California, San Diego, La Jolla, USA. wcampana@ucsd.edu
Mohiuddin
L
L
Misasi
R
R
O'Brien
J S
JS
Calcutt
N A
NA
D000367:Age Factors; D000818:Animals; D002478:Cells, Cultured; D005260:Female; D005727:Ganglia, Spinal / Q000166:cytology; D006023:Glycoproteins / Q000138:chemical synthesis / Q000494:pharmacology*; D066298:In Vitro Techniques; D009045:Motor Endplate / Q000187:drug effects / Q000502:physiology; D009046:Motor Neurons / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D009414:Nerve Growth Factors / Q000138:chemical synthesis / Q000494:pharmacology*; D009416:Nerve Regeneration / Q000187:drug effects*; D009475:Neurons, Afferent / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D049231:Saposins
D006023:Glycoproteins; D009414:Nerve Growth Factors; C483347:Psap protein, rat; D049231:Saposins; C101246:prosaptide