title: Acf1, the largest subunit of CHRAC, regulates ISWI-induced nucleosome remodelling.
abstract: The chromatin accessibility complex (CHRAC) was originally defined biochemically as an ATP-dependent 'nucleosome remodelling' activity. Central to its activity is the ATPase ISWI, which catalyses the transfer of histone octamers between DNA segments in cis. In addition to ISWI, four other potential subunits were observed consistently in active CHRAC fractions. We have now identified the p175 subunit of CHRAC as Acf1, a protein known to associate with ISWI in the ACF complex. Interaction of Acf1 with ISWI enhances the efficiency of nucleosome sliding by an order of magnitude. Remarkably, it also modulates the nucleosome remodelling activity of ISWI qualitatively by altering the directionality of nucleosome movements and the histone 'tail' requirements of the reaction. The Acf1-ISWI heteromer tightly interacts with the two recently identified small histone fold proteins CHRAC-14 and CHRAC-16. Whether topoisomerase II is an integral subunit has been controversial. Refined analyses now suggest that topoisomerase II should not be considered a stable subunit of CHRAC. Accordingly, CHRAC can be molecularly defined as a complex consisting of ISWI, Acf1, CHRAC-14 and CHRAC-16.
authors.affiliation: Adolf-Butenandt-Institut, Molekularbiologie, Schillerstrasse 44, D-80336 München, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Eberharter
authors.forename: S
authors.initials: S
authors.lastname: Ferrari
authors.forename: G
authors.initials: G
authors.lastname: Längst
authors.forename: T
authors.initials: T
authors.lastname: Straub
authors.forename: A
authors.initials: A
authors.lastname: Imhof
authors.forename: P
authors.initials: P
authors.lastname: Varga-Weisz
authors.forename: M
authors.initials: M
authors.lastname: Wilm
authors.forename: P B
authors.initials: PB
authors.lastname: Becker
mesh_terms: D000251:Adenosine Triphosphatases / Q000502:physiology*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D017931:DNA Primers; D004250:DNA Topoisomerases, Type II / Q000378:metabolism; D004330:Drosophila; D029721:Drosophila Proteins; D006657:Histones / Q000378:metabolism; D009707:Nucleosomes / Q000378:metabolism*; D011233:Precipitin Tests; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism; D014157:Transcription Factors / Q000302:isolation & purification / Q000378:metabolism / Q000502:physiology*
chemical_list: C120419:Acf protein, Drosophila; D017931:DNA Primers; D029721:Drosophila Proteins; D006657:Histones; C086468:ISWI protein; D009707:Nucleosomes; D011994:Recombinant Proteins; D014157:Transcription Factors; D000251:Adenosine Triphosphatases; D004250:DNA Topoisomerases, Type II