Centrosome abnormalities and genomic instability by episomal expression of human papillomavirus type 16 in raft cultures of human keratinocytes.
Primary human keratinocytes with ectopic expression of high-risk human papillomavirus (HPV) E6 and E7 oncoproteins display abnormal centrosome numbers, multipolar mitoses, and aneusomy. However, it has not been explored whether these abnormalities can occur in cells containing HPV episomes where E6 and E7 expression is under viral transcriptional control. Here, we demonstrate that centrosome abnormalities and genomic instability occur in organotypic raft cultures of human keratinocytes with episomal HPV-16 even at low copy numbers. We conclude that HPV-16 DNA, when maintained as an episome, can disturb centrosome homeostasis and subvert genomic integrity of the host cell during early stages of the viral infection.
Duensing
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S
Department of Pathology and Harvard Center for Cancer Biology, Solid Tumor Cytogenetics, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA.
Duensing
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A
Flores
E R
ER
Do
A
A
Lambert
P F
PF
Münger
K
K
D002478:Cells, Cultured; D002503:Centromere / Q000235:genetics* / Q000473:pathology; D002873:Chromosome Fragility; D006801:Humans; D015603:Keratinocytes / Q000502:physiology / Q000821:virology*; D027383:Papillomaviridae; D030361:Papillomavirus Infections / Q000235:genetics*; D010957:Plasmids; D014412:Tumor Virus Infections / Q000235:genetics*