title: Aberrant methylation of the adenomatous polyposis coli (APC) gene promoter 1A in breast and lung carcinomas.
abstract: The adenomatous polyposis coli (APC) gene is a tumor suppressor gene associated with both familial and sporadic cancer. Despite high rates of allelic loss in lung and breast cancers, point mutations of the APC gene are infrequent in these cancer types. Aberrant methylation of the APC promoter 1A occurs in some colorectal and gastric malignancies, and we investigated whether the same mechanism occurs in lung and breast cancers. The methylation status of the APC gene promoter 1A was analyzed in 77 breast, 50 small cell (SCLC), and 106 non-small cell (NSCLC) lung cancer tumors and cell lines and in 68 nonmalignant tissues by methylation-specific PCR. Expression of the APC promoter 1A transcript was examined in a subset of cell lines by reverse transcription-PCR, and loss of heterozygosity at the gene locus was analyzed by the use of 12 microsatellite and polymorphic markers. Statistical tests were two-sided. Promoter 1A was methylated in 34 of 77 breast cancer tumors and cell lines (44%), in 56 of 106 NSCLC tumors and cell lines (53%), in 13 of 50 SCLC cell lines (26%), and in 3 of 68 nonmalignant samples (4%). Most cell lines tested contained the unmethylated or methylated form exclusively. In 27 cell lines tested, there was complete concordance between promoter methylation and silencing of its transcript. Demethylation with 5-aza-2'-deoxycytidine treatment restored transcript 1A expression in all eight methylated cell lines tested. Loss of heterozygosity at the APC locus was observed in 85% of SCLCs, 83% of NSCLCs, and 63% of breast cancer cell lines. The frequency of methylation in breast cancers increased with tumor stage and size. In summary, aberrant methylation of the 1A promoter of the APC gene and loss of its specific transcript is frequently present in breast and NSCLC cancers and cell lines and, to a lesser extent, in SCLC cell lines. Our findings may be of biological and clinical importance.
authors.affiliation: Hamon Center for Therapeutic Oncology Research, and Department of Pathology, University of Texas Southwestern Medical Center, Dallas, Texas 75390-8593, USA.
authors.forename: A K
authors.initials: AK
authors.lastname: Virmani
authors.forename: A
authors.initials: A
authors.lastname: Rathi
authors.forename: U G
authors.initials: UG
authors.lastname: Sathyanarayana
authors.forename: A
authors.initials: A
authors.lastname: Padar
authors.forename: C X
authors.initials: CX
authors.lastname: Huang
authors.forename: H T
authors.initials: HT
authors.lastname: Cunnigham
authors.forename: A J
authors.initials: AJ
authors.lastname: Farinas
authors.forename: S
authors.initials: S
authors.lastname: Milchgrub
authors.forename: D M
authors.initials: DM
authors.lastname: Euhus
authors.forename: M
authors.initials: M
authors.lastname: Gilcrease
authors.forename: J
authors.initials: J
authors.lastname: Herman
authors.forename: J D
authors.initials: JD
authors.lastname: Minna
authors.forename: A F
authors.initials: AF
authors.lastname: Gazdar
mesh_terms: D025601:Adenomatous Polyposis Coli Protein; D017398:Alternative Splicing; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000473:pathology; D002895:Chromosomes, Human, Pair 5 / Q000235:genetics; D003598:Cytoskeletal Proteins / Q000235:genetics*; D019175:DNA Methylation; D004273:DNA, Neoplasm / Q000235:genetics / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D019656:Loss of Heterozygosity; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D018895:Microsatellite Repeats; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012334:RNA, Neoplasm / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
chemical_list: D025601:Adenomatous Polyposis Coli Protein; D003598:Cytoskeletal Proteins; D004273:DNA, Neoplasm; D012334:RNA, Neoplasm