title: The application of Artocarpus integer seed lectin-M in the detection and isolation of selective human serum acute-phase proteins and immunoglobulins.
abstract: Champedak (Artocarpus integer) lectin-M is a lectin with high specificity and affinity for the core-mannosyl residues of the N-linked oligosaccharides of glycoproteins. We have studied the interaction of the champedak seed lectin with human serum glycoproteins that were resolved by 2-dimensional (2-D) gel electrophoresis. The lectin demonstrated strong interaction with haptoglobin beta chain, orosomucoid, alpha1-antitrypsin, alpha2-HS glycoprotein, transferrin, hemopexin, alpha1B-glycoprotein, and the heavy chains of IgA, IgM and IgG of the human serum. With exceptions of the heavy chains of the immunoglobulins and alpha1B-glycoprotein, all the other lectin-M-probed glycopeptides are acute-phase proteins. The use of champedak lectin-M to probe for serum glycoproteins that were separated in a 2-D gel electrophoresis and Western blotting technique may be conveniently applied to analyse the acute-phase and humoral immune responses simultaneously. Subjecting human serum to immobilised-lectin-M affinity chromatography was able to isolate intact haptoglobin, alpha1-antitrypsin, alpha1B-glycoprotein, hemopexin and IgA.
authors.affiliation: Department of Biochemistry, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
authors.forename: O H
authors.initials: OH
authors.lastname: Hashim
authors.forename: F
authors.initials: F
authors.lastname: Ahmad
authors.forename: A S
authors.initials: AS
authors.lastname: Shuib
mesh_terms: D000209:Acute-Phase Proteins / Q000378:metabolism*; D006023:Glycoproteins / Q000378:metabolism; D006801:Humans; D007136:Immunoglobulins / Q000378:metabolism*; D007369:Interferon Inducers / Q000378:metabolism*; D037102:Lectins / Q000378:metabolism*; D029586:Moraceae; D010084:Oxidation-Reduction; D037121:Plant Lectins; D012639:Seeds
chemical_list: D000209:Acute-Phase Proteins; D006023:Glycoproteins; D007136:Immunoglobulins; D007369:Interferon Inducers; D037102:Lectins; D037121:Plant Lectins; C043696:jacalin