title: Reduced hepatic uptake and intestinal excretion of organic cations in mice with a targeted disruption of the organic cation transporter 1 (Oct1 [Slc22a1]) gene.
abstract: The polyspecific organic cation transporter 1 (OCT1 [SLC22A1]) mediates facilitated transport of small (hydrophilic) organic cations. OCT1 is localized at the basolateral membrane of epithelial cells in the liver, kidney, and intestine and could therefore be involved in the elimination of endogenous amines and xenobiotics via these organs. To investigate the pharmacologic and physiologic role of this transport protein, we generated Oct1 knockout (Oct1(-/-)) mice. Oct1(-/-) mice appeared to be viable, healthy, and fertile and displayed no obvious phenotypic abnormalities. The role of Oct1 in the pharmacology of substrate drugs was studied by comparing the distribution and excretion of the model substrate tetraethylammonium (TEA) after intravenous administration to wild-type and Oct1(-/-) mice. In Oct1(-/-) mice, accumulation of TEA in liver was four to sixfold lower than in wild-type mice, whereas direct intestinal excretion of TEA was reduced about twofold. Excretion of TEA into urine over 1 h was 53% of the dose in wild-type mice, compared to 80% in knockout mice, probably because in Oct1(-/-) mice less TEA accumulates in the liver and thus more is available for rapid excretion by the kidney. In addition, we found that absence of Oct1 leads to decreased liver accumulation of the anticancer drug metaiodobenzylguanidine and the neurotoxin 1-methyl-4-phenylpyridium. In conclusion, our data show that Oct1 plays an important role in the uptake of organic cations into the liver and in their direct excretion into the lumen of the small intestine.
authors.affiliation: Division of Experimental Therapy, 1066 CX Amsterdam, The Netherlands.
authors.forename: J W
authors.initials: JW
authors.lastname: Jonker
authors.forename: E
authors.initials: E
authors.lastname: Wagenaar
authors.forename: C A
authors.initials: CA
authors.lastname: Mol
authors.forename: M
authors.initials: M
authors.lastname: Buitelaar
authors.forename: H
authors.initials: H
authors.lastname: Koepsell
authors.forename: J W
authors.initials: JW
authors.lastname: Smit
authors.forename: A H
authors.initials: AH
authors.lastname: Schinkel
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D002412:Cations / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000378:metabolism; D017136:Ion Transport / Q000235:genetics; D008099:Liver / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D027702:Organic Cation Transporter 1
chemical_list: D002352:Carrier Proteins; D002412:Cations; D008565:Membrane Proteins; D027702:Organic Cation Transporter 1