Evaluation of the general suitability of the rat for the micronucleus assay: the effect of cyclophosphamide in 14 strains.
To evaluate the general suitability of the rat for the micronucleus assay, we conducted the assay in males of 14 different strains, 13 inbred (ACI, BN, BUF, COP, DRH, F344, IS, LEW, RCS, SHR, WAG, WKYO, WTC) and 1 outbred (SD), using cyclophosphamide as the test chemical. Cyclophosphamide at 0 (vehicle), 5, 10, or 20mg/kg per day was administered orally twice, 24h apart, to five rats per dosage group. Bone marrow and peripheral blood were collected 24h after the second treatment. All 14 strains showed a positive response to cyclophosphamide, with slight differences in sensitivity. We concluded that the rat is suitable for the micronucleus assay regardless of strain.
Hamada
S
S
Central Research Laboratory, SSP Co. Ltd., 1143 Nanpeidai, Narita, Chiba 286-8511, Japan. shuichi.hamada@ssp.co.jp
Yamasaki
K I
KI
Nakanishi
S
S
Omori
T
T
Serikawa
T
T
Hayashi
M
M
D000284:Administration, Oral; D000818:Animals; D021461:Animals, Outbred Strains; D001854:Bone Marrow Cells / Q000187:drug effects; D003520:Cyclophosphamide / Q000008:administration & dosage / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D015162:Micronucleus Tests / Q000379:methods* / Q000592:standards; D009153:Mutagens / Q000008:administration & dosage / Q000633:toxicity*; D051381:Rats; D011919:Rats, Inbred Strains; D012156:Reticulocytes / Q000187:drug effects; D012680:Sensitivity and Specificity; D013045:Species Specificity
D009153:Mutagens; D003520:Cyclophosphamide