Production, characterisation and applications of monoclonal antibodies to porcine circovirus 2.
The production, preliminary characterisation and applications of monoclonal antibodies (mabs) against six porcine circovirus 2 isolates are described. A total of 14 stable hybridomas were produced, of which 7 were characterised. All of the mabs characterised were of IgG isotype. All the mabs tested reacted by IIF with acetone-fixed cell cultures infected with PCV2 isolates from Canada, France, Spain, Denmark, USA and UK. No cross-reactivity with a porcine circovirus 1 field isolate was demonstrated using the panel of mabs tested. In addition, one of the seven mabs tested demonstrated neutralising activity against PCV2 isolates from Canada and France. The use of selected PCV2-specific mabs for the development of virus detection methodologies is described.
McNeilly
F
F
Virology Department, Department of Agriculture and Rural Development for N. Ireland, Belfast, UK.
McNair
I
I
Mackie
D P
DP
Meehan
B M
BM
Kennedy
S
S
Moffett
D
D
Ellis
J
J
Krakowka
S
S
Allan
G M
GM
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology* / Q000302:isolation & purification; D000914:Antibodies, Viral / Q000096:biosynthesis / Q000276:immunology / Q000302:isolation & purification; D002478:Cells, Cultured / Q000821:virology; D018173:Circoviridae Infections / Q000662:veterinary / Q000821:virology; D017925:Circovirus / Q000145:classification / Q000276:immunology* / Q000302:isolation & purification; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D006825:Hybridomas / Q000276:immunology; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000276:immunology* / Q000302:isolation & purification; D007668:Kidney; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D013552:Swine; D013553:Swine Diseases / Q000821:virology; D016707:Tissue Fixation
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D007074:Immunoglobulin G