Notl-Msell methylation-sensitive amplied fragment length polymorhism for DNA methylation analysis of human cancers.
We have applied a methylation-sensitive restriction endonuclease, NotI, to the existing amplified fragment length polymorphism (AFLP) method and developed NotI-MseI methylation-sensitive-AFLP (MS-AFLP). NotI-MseI MS-AFLP allows the analysis of DNA methylation alterations at the NotI sites scattered over the genome. Hypermethylation and hypomethylation are visualized by the decrease and increase in the band intensity of DNA fingerprints. Identification of consistent changes can be facilitated through parallel electrophoresis of multiple samples. DNA fragments exhibiting alterations can be cloned from fingerprint bands by amplification of gel-eluted DNA with the same pair of primers used for radioactive fingerprint presentation. Fluorescent NotI-MseI MS-AFLP offers a safer method of studying the alterations in DNA methylation, and may be applied to the hybridization of DNA microarrays in the future. Using NotI-MseI MS-AFLP, we observed frequent hypomethylation of a satellite DNA repeat sequence in a majority of breast tumors.
Yamamoto
F
F
The Burnham Institute, La Jolla Cancer Research Center, CA 92037, USA. fyamamoto@burnham.org
Yamamoto
M
M
Soto
J L
JL
Kojima
E
E
Wang
E N
EN
Perucho
M
M
Sekiya
T
T
Yamanaka
H
H
D015139:Blotting, Southern / Q000379:methods; D001943:Breast Neoplasms / Q000737:chemistry / Q000235:genetics; D016172:DNA Fingerprinting / Q000379:methods; D019175:DNA Methylation; D004273:DNA, Neoplasm / Q000737:chemistry* / Q000235:genetics*; D004276:DNA, Satellite / Q000737:chemistry / Q000235:genetics; D015252:Deoxyribonucleases, Type II Site-Specific; D005260:Female; D005456:Fluorescent Dyes; D006801:Humans; D008175:Lung Neoplasms / Q000737:chemistry / Q000235:genetics; D008297:Male; D009369:Neoplasms / Q000737:chemistry* / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D011471:Prostatic Neoplasms / Q000737:chemistry / Q000235:genetics; D015203:Reproducibility of Results
D004273:DNA, Neoplasm; D004276:DNA, Satellite; D005456:Fluorescent Dyes; C055927:endodeoxyribonuclease MseI; D015252:Deoxyribonucleases, Type II Site-Specific; C038565:GCGGCCGC-specific type II deoxyribonucleases