Clonal analysis of B cells in the osteoarthritis synovium.
OBJECTIVE
Cellular and humoral immunity to collagen and cartilage proteoglycan were shown in patients with osteoarthritis (OA). Inflammatory infiltration containing T and B lymphocytes and macrophages, which are HLA-DR positive, is often seen in the synovial membrane of patients with OA. An analysis of the DNA restriction enzyme patterns of T lymphocytes from the OA synovium showed an oligoclonal pattern of T cell receptor beta chain gene rearrangements. No similar studies of B cell clonality have previously been performed. This study aimed at determining the clonal characteristics of the B cells in the OA synovium.


METHODS
A reverse transcriptase-polymerase chain reaction of the immunoglobulin transcripts of B cells in the synovial membranes from six patients with OA was performed and the products were analysed by a single strand conformation polymorphism analysis.


RESULTS
Several dominant bands were seen in all samples and some of the dominant bands were common among the two or three separate regions of each synovial sample.


CONCLUSIONS
Infiltrated B cells are oligoclonal, and an antigen driven immune response may play a part in the progression of the disease process in OA.
Shiokawa
S
S
Department of Clinical Immunology, Medical Institute of Bioregulation, Kyushu University, Tsurumihara 4546, Beppu, Oita 874-0838, Japan. sahasu@tsurumi.beppu.kyushu-u.ac.jp
Matsumoto
N
N
Nishimura
J
J
D001402:B-Lymphocytes / Q000502:physiology*; D001854:Bone Marrow Cells / Q000502:physiology; D002999:Clone Cells; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000502:physiology; D010003:Osteoarthritis / Q000276:immunology*; D018807:Polymorphism, Single-Stranded Conformational; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013583:Synovial Membrane / Q000276:immunology*