title: Ancient and recent duplications of the rainbow trout Wilms' tumor gene.
abstract: The Wilms' tumor suppressor (WT1) gene plays an important role in the development and functioning of the genitourinary system, and mutations in this gene are associated with nephroblastoma formation in humans. Rainbow trout (Oncorhynchus mykiss) is one of the rare animal models that readily form nephroblastomas, yet trout express three distinct WT1 genes, one of which is duplicated and inherited tetrasomically. Sequence analyses suggest an ancient gene duplication in the common ancestor of bony fishes resulted in the formation of two WT1 gene families, that conserve the splicing variations of tetrapod WT1, and a second duplication event occurred in the trout lineage. The WT1 genes of one family map to linkage groups 6 and 27 in the trout genome map. Reverse transcribed polymerase chain reaction (RT-PCR) expression analysis demonstrated little difference in W
authors.affiliation: School of Biological Sciences, Washington State University, Pullman 99164-4236, USA.
authors.forename: J P
authors.initials: JP
authors.lastname: Brunelli
authors.forename: B D
authors.initials: BD
authors.lastname: Robison
authors.forename: G H
authors.initials: GH
authors.lastname: Thorgaard
mesh_terms: D000483:Alleles; D017398:Alternative Splicing / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D019143:Evolution, Molecular; D005393:Fish Diseases / Q000235:genetics / Q000473:pathology; D020440:Gene Duplication; D020869:Gene Expression Profiling; D020131:Genes, Duplicate / Q000235:genetics; D016162:Genes, Wilms Tumor / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D017686:Oncorhynchus mykiss / Q000235:genetics*; D010802:Phylogeny; D011110:Polymorphism, Genetic / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D009396:Wilms Tumor / Q000235:genetics / Q000473:pathology / Q000662:veterinary
chemical_list: D012333:RNA, Messenger