title: Changes in subgingival microflora and humoral immune response following periodontal therapy.
abstract: OBJECTIVE
  To investigate the effect of scaling and root planing (SRP) on the microflora and humoral immune response in adult periodontitis.
  
  
  METHODS
  Clinical measurements, subgingival plaque samples, gingival crevicular fluid and sera were taken from 4 sites in 28 adult periodontitis patients before and after SRP. Polymerase chain reaction was used to determine the presence of A. actinomycetemcomitans, P. gingivalis, B. forsythus, P. intermedia, and T. denticola. ELISA was used to investigate the systemic and local antibody titres to these organisms, and thiocyanate dissociation for the determination of serum antibody avidity.
  
  
  RESULTS
  SRP produced a good clinical improvement. On a subject basis there was little significant change in the microflora. However, on a site basis, there were significant reductions in P. intermedia, B. forsythus and T. denticola. There was little change in systemic and local antibody titres following SRP, although there was a significant reduction in antibody avidity to P. gingivalis and P. intermedia
  
  
  CONCLUSIONS
  Post-therapy clinical improvement was associated with a reduction in bacterial prevalence, but statistical significance was only reached at a site level and this microbial reduction was not significant for all organisms. No significant post-therapy effects on the humoral immune response were noted other than a reduced antibody avidity to P. gingivalis and P. intermedia. The lack of a clear pattern in the humoral immune response may reflect a failure of the host response to produce adequate levels of biologically functional antibodies, and complex interactions between the subgingival flora and the host response.
authors.affiliation: Periodontal and Oral Immunology Research Group, University of Glasgow Dental School, 378 Sauchiehall Street, Glasgow, G2 3JZ Scotland, UK.
authors.forename: I B
authors.initials: IB
authors.lastname: Darby
authors.forename: J
authors.initials: J
authors.lastname: Mooney
authors.forename: D F
authors.initials: DF
authors.lastname: Kinane
mesh_terms: D000328:Adult; D000368:Aged; D000907:Antibodies, Bacterial / Q000276:immunology*; D015169:Colony Count, Microbial; D012534:Dental Scaling; D005260:Female; D005881:Gingiva / Q000382:microbiology*; D006801:Humans; D018928:Immunity, Mucosal; D008297:Male; D008875:Middle Aged; D010518:Periodontitis / Q000276:immunology* / Q000382:microbiology* / Q000628:therapy; D016745:Root Planing; D012463:Saliva / Q000276:immunology / Q000382:microbiology; D016896:Treatment Outcome
chemical_list: D000907:Antibodies, Bacterial