Hypothyroidism in the developing rat brain is associated with marked oxidative stress and aberrant intraneuronal accumulation of neurofilaments.
The effects of hypothyroidism on parameters of oxidative stress and on intraneuronal distribution of neurofilaments have been investigated in the developing rat brain. Progressive hypothyroidism during the first 4 weeks of postnatal development led to an increase in superoxide dismutase and catalase activity, decline in the level of glutathione and mitochondrial cytochrome c oxidase activity and increase in the level of .OH radical along with enhanced protein carbonylation and lipid peroxidation. Immunocytochemical staining of cryostat sections of normal and hypothyroid cerebella from 25 day postnatal rats with anti neurofilament (NF) light chain (L) antibody showed aberrant accumulation of neurofilaments in the perikaryon of the hypothyroid Purkinje neurons in contrast to relatively uniform distribution in the controls. The morphological and biochemical alterations in the neurons of the developing hypothyroid brain are comparable to those seen in several neurodegenerative diseases.
Rahaman
S O
SO
Division of Neurobiology, Indian Institute of Chemical Biology, 4 Raja S.C. Mullick Road, Jadavpur, Calcutta 700 032, India.
Ghosh
S
S
Mohanakumar
K P
KP
Das
S
S
Sarkar
P K
PK
D000818:Animals; D000831:Animals, Newborn / Q000002:abnormalities / Q000378:metabolism; D001921:Brain / Q000254:growth & development* / Q000473:pathology / Q000503:physiopathology; D003576:Electron Transport Complex IV / Q000378:metabolism; D005981:Glutathione Synthase / Q000378:metabolism; D017665:Hydroxyl Radical / Q000378:metabolism; D007037:Hypothyroidism / Q000150:complications* / Q000378:metabolism / Q000473:pathology; D007150:Immunohistochemistry; D015227:Lipid Peroxidation / Q000502:physiology; D008928:Mitochondria / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism; D016900:Neurofilament Proteins / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism* / Q000473:pathology; D018384:Oxidative Stress / Q000502:physiology*; D011689:Purkinje Cells / Q000378:metabolism / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000378:metabolism; D013963:Thyroid Hormones / Q000172:deficiency*
D009419:Nerve Tissue Proteins; D016900:Neurofilament Proteins; D013963:Thyroid Hormones; D017665:Hydroxyl Radical; D013482:Superoxide Dismutase; D003576:Electron Transport Complex IV; D005981:Glutathione Synthase