title: Enzyme-linked immunosorbent assay. II. Quantitative assay of protein antigen, immunoglobulin G, by means of enzyme-labelled antigen and antibody-coated tubes.
abstract: Alkaline phosphatase from calf intestinal mucosa has been conjugated to a protein antigen, rabbit IgG. Such conjugates, prepared by glutardialdehyde, have been used in a competitive solid phase immunoassay. In this test native antigen inhibits the binding of the conjugate to homologous antibodies adsorbed to plastic tubes. Using this assay 1-100 ng/ml of the antigen could be determined.
authors.affiliation: Department of Immunology, Wenner-Gren Institute, University of Stockholm, Stockholm, Sweden.
authors.forename: E
authors.initials: E
authors.lastname: Engvall
authors.forename: K
authors.initials: K
authors.lastname: Jonsson
authors.forename: P
authors.initials: P
authors.lastname: Perlmann
mesh_terms: D000327:Adsorption; D000469:Alkaline Phosphatase / Q000276:immunology*; D000818:Animals; D000906:Antibodies / Q000032:analysis*; D000918:Antibody Specificity; D000941:Antigens / Q000032:analysis*; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D007074:Immunoglobulin G; D007413:Intestinal Mucosa / Q000201:enzymology; D011817:Rabbits; D012756:Sheep
chemical_list: D000906:Antibodies; D000941:Antigens; D007074:Immunoglobulin G; D000469:Alkaline Phosphatase