title: Relationship between degree of viremia and disease manifestation in calves with experimentally induced bovine viral diarrhea virus infection.
abstract: OBJECTIVE
  To compare degree of viremia and disease manifestations in calves with type-I and -II bovine viral diarrhea virus (BVDV) infection.
  
  
  METHODS
  16 calves.
  
  
  METHODS
  Colostrum-deprived calves obtained immediately after birth were assigned to 1 control and 3 treatment groups (4 calves/group). Calves in treatment groups were inoculated (day 0) by intranasal instillation of 10(7) median tissue culture infective dose BVDV 890 (type II), BVDV 7937 (type II), or BVDV TGAN (type I). Blood cell counts and virus isolation from serum and leukocytes were performed daily, whereas degree of viremia was determined immediately before and 4, 6, 8, and 12 days after inoculation. Calves were euthanatized on day 12, and pathologic, virologic, and immunohistochemical examinations were performed.
  
  
  RESULTS
  Type-II BVDV 890 induced the highest degree of viremia, and type-I BVDV TGAN induced the lowest. Virus was isolated more frequently and for a longer duration in calves inoculated with BVDV 890. A parallel relationship between degree of viremia and rectal temperature and an inverse relationship between degree of viremia and blood cell counts was observed. Pathologic and immunohistochemical examinations revealed more pronounced lesions and more extensive distribution of viral antigen in calves inoculated with type-II BVDV.
  
  
  CONCLUSIONS
  Degree of viremia induced during BVDV infection is associated with severity of clinical disease. Isolates of BVDV that induce a high degree of viremia may be more capable of inducing clinical signs of disease. Strategies (eg, vaccination) that reduce viremia may control clinical signs of acute infection with BVDV.
authors.affiliation: Department of Large Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University, East Lansing 48824-1314, USA.
authors.forename: P H
authors.initials: PH
authors.lastname: Walz
authors.forename: T G
authors.initials: TG
authors.lastname: Bell
authors.forename: J L
authors.initials: JL
authors.lastname: Wells
authors.forename: D L
authors.initials: DL
authors.lastname: Grooms
authors.forename: L
authors.initials: L
authors.lastname: Kaiser
authors.forename: R K
authors.initials: RK
authors.lastname: Maes
authors.forename: J C
authors.initials: JC
authors.lastname: Baker
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000956:Antigens, Viral / Q000032:analysis; D001831:Body Temperature; D001853:Bone Marrow / Q000473:pathology / Q000821:virology; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000097:blood / Q000473:pathology* / Q000821:virology; D002417:Cattle; D001908:Diarrhea Viruses, Bovine Viral / Q000472:pathogenicity*; D007150:Immunohistochemistry / Q000662:veterinary; D007958:Leukocyte Count / Q000662:veterinary; D008297:Male; D010976:Platelet Count / Q000662:veterinary; D013950:Thymus Gland / Q000473:pathology / Q000821:virology; D014766:Viremia / Q000473:pathology / Q000662:veterinary* / Q000821:virology
chemical_list: D000956:Antigens, Viral