A 10-kda fragment of fibronectin type III domain is a neutrophil chemoattractant purified from conditioned medium of rat granulation tissue.
A neutrophil chemoattractant has been purified from the conditioned medium of granulation tissue obtained from carrageenan-induced inflammation in rats. The purified chemoattractant was a basic protein with a molecular mass of 10 kDa on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) under reducing and non-reducing conditions. NH2-terminal amino acid sequence of the purified 10-kDa protein was identical with the sequence of rat fibronectin starting from the residue Thr585, indicating that the purified 10-kDa chemoattractant is a fragment derived from the NH2-terminal type III domain of rat fibronectin.
Shiota
S
S
Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, Japan.
Takano
K
K
Nakagawa
H
H
D000595:Amino Acid Sequence; D000818:Animals; D002630:Chemotactic Factors / Q000302:isolation & purification / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D017077:Culture Media, Conditioned; D046508:Culture Techniques; D005353:Fibronectins / Q000302:isolation & purification / Q000494:pharmacology*; D006097:Granulation Tissue / Q000378:metabolism*; D008297:Male; D008970:Molecular Weight; D009504:Neutrophils / Q000187:drug effects*; D010446:Peptide Fragments / Q000302:isolation & purification / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
D002630:Chemotactic Factors; D017077:Culture Media, Conditioned; D005353:Fibronectins; D010446:Peptide Fragments