In vivo dynamics of human stem cell repopulation in NOD/SCID mice.
Primitive human hematopoietic cells can be assayed on the basis of their ability to repopulate immune-deficient NOD/SCID mice and have been termed SCID repopulating cells (SRCs). The in vivo biological fate of individual SRCs can be tracked by following the unique retroviral insertion site in the progency of transduced SRCs. Distinct human SRCs were identified that differ in the proliferative and self-renewal capacity indicating that the primitive cell compartment is functionally heterogeneous.
Dick
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JE
Programs in Cancer/Blood Research and Gene Therapy, Hospital for Sick Children, 555 University Avenue, Toronto, Ontario, Canada, M5G 1X8. dick@sickkids.on.ca
Guenechea
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Gan
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Dorrell
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D000818:Animals; D002455:Cell Division; D002999:Clone Cells; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D017930:Genes, Reporter; D005819:Genetic Markers; D005822:Genetic Vectors / Q000235:genetics; D006085:Graft Survival; D049452:Green Fluorescent Proteins; D006410:Hematopoiesis; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D008164:Luminescent Proteins / Q000032:analysis / Q000235:genetics; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D011533:Proviruses / Q000235:genetics; D011828:Radiation Chimera; D011993:Recombinant Fusion Proteins / Q000032:analysis; D012190:Retroviridae / Q000235:genetics; D016511:Severe Combined Immunodeficiency / Q000473:pathology / Q000628:therapy; D013045:Species Specificity; D014162:Transfection; D019076:Transgenes; D014183:Transplantation, Heterologous; D016662:Virus Integration / Q000235:genetics
D004279:DNA, Viral; D005819:Genetic Markers; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins