Antioxidant and pro-oxidant capacity of catecholamines and related compounds. Effects of hydrogen peroxide on glutathione and sphingomyelinase activity in pheochromocytoma PC12 cells: potential relevance to age-related diseases.
The antioxidant and pro-oxidant capacity of catecholamines (CA) and related compounds were analyzed using the oxygen radical absorbance capacity (ORAC) assay. In the assay 2,2'-azobis (2-amidino-propane) dihydrochloride (AAPH), a peroxyl radical generator, ROO*; H2O2-Cu2+, mainly a hydroxyl radical generator, *OH; and Cu2+ a transition metal were used. The antioxidant effect of CA and its related compounds were in the order: neurotransmitters: dopamine (DA), norepinephrine (NE) > metabolites > amino acid precursors as measured by using AAPH. The antioxidant effect of CA and related compounds as measured by using AAPH were linearly correlated with concentration, while the antioxidant effect of CA in scavenging *OH produced by H2O2-Cu2+ increased proportionally to concentration at low concentration, but after reaching a maximum declined with increasing concentration. In the presence of Cu2+, CA acted as pro-oxidant. Glutathione (GSH) acted as a pro-oxidant when H2O2-Cu2+ or when Cu2+ alone was used as an oxidant and showed much higher pro-oxidant effect than DA, which could have relevance in the vulnerability of dopaminergic neurons to oxidative stress in the aging and aging related diseases. The antioxidant capacity of CA and many related compounds seems to be correlated with the numbers of hydroxyl groups and their position on the benzoic ring. The O-methylation and sulfate conjugation of the hydroxyl substitution inactivates both the antioxidant and pro-oxidant activities of CA. Our results show that oxidative stress induced by low (5 microM) or high (300 microM) doses H2O2 in pheochromocytoma PC12 cells significantly up-regulate the activity of Mg-dependent neutral sphingomyelinase (Sase), and significantly decreased GSH.
Sofic
E
E
Phytochemical Laboratory, Jean Mayer USDA Human Nutrition Research Center on Aging, Tufts University, Boston, MA, USA.
Denisova
N
N
Youdim
K
K
Vatrenjak-Velagic
V
V
De Filippo
C
C
Mehmedagic
A
A
Causevic
A
A
Cao
G
G
Joseph
J A
JA
Prior
R L
RL
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001681:Biological Assay; D002395:Catecholamines / Q000494:pharmacology*; D002518:Ceramides / Q000096:biosynthesis; D003300:Copper / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005609:Free Radicals / Q000378:metabolism; D005978:Glutathione / Q000187:drug effects* / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D017665:Hydroxyl Radical / Q000378:metabolism; D019636:Neurodegenerative Diseases / Q000201:enzymology* / Q000503:physiopathology; D016877:Oxidants / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology*; D016716:PC12 Cells / Q000187:drug effects* / Q000378:metabolism; D010545:Peroxides / Q000378:metabolism; D051381:Rats; D013108:Sphingomyelin Phosphodiesterase / Q000187:drug effects* / Q000378:metabolism; D013109:Sphingomyelins / Q000378:metabolism
D000975:Antioxidants; D002395:Catecholamines; D002518:Ceramides; D005609:Free Radicals; D016877:Oxidants; D010545:Peroxides; D013109:Sphingomyelins; C049375:perhydroxyl radical; D017665:Hydroxyl Radical; D003300:Copper; D006861:Hydrogen Peroxide; D013108:Sphingomyelin Phosphodiesterase; D005978:Glutathione