[Analysis of the structure-functional features of a highly-repeating centromere sequence from the Fringilla coelebs L. (Aves: Passeriformes) genome].
A highly repetitive centromeric Fringilla coelebs PstI (FCP) element was cloned and sequenced. The FCP tandem repeats with unit 505 or 506 nt accounted for about 0.9% of the entire genome and had 57% GC. Direct genomic sequencing with FCP-specific primers and ThermoFidelase 2A revealed the consensus sequence and the five most common single-nucleotide polymorphisms (SNPs) for the FCP unit. FCP may be transcribed and may play a role in spatial arrangement of the genome.
Malykh
A G
AG
Zhurov
V G
VG
Saĭfitdinova
A F
AF
Deriusheva
S E
SE
Gaginskaia
E R
ER
D000818:Animals; D001483:Base Sequence; D001717:Birds / Q000235:genetics*; D002503:Centromere; D017931:DNA Primers; D020641:Polymorphism, Single Nucleotide; D012091:Repetitive Sequences, Nucleic Acid
D017931:DNA Primers