Protective CTL response is induced in the absence of CD4+ T cells and IFN-gamma by gene gun DNA vaccination with a minigene encoding a CTL epitope of Listeria monocytogenes.
Our work was undertaken to learn the mechanism of induction of protective cytotoxic T lymphocytes (CTL) by gene gun DNA vaccination with p91m encoding an H-2Kd-restricted T cell epitope of listeriolysin O (LLO). Vaccination with p91m induced vigorous antigen-specific CD8+ CTL that produce IFN-gamma and was able to confer partial protection against listerial challenge. However, the p91m-induced protective immunity was revealed to be independent of the IFN-gamma and CD4+ T cell help. The CTL induction is also suggested to require neither adjuvant activity of the plasmid used nor IFN-gamma. The data may be feasible for the design of CTL inducing vaccines in various immunodeficiencies.
Yoshida
A
A
Department of Microbiology and Immunology, Hamamatsu University School of Medicine, 431-3192, Hamamatsu, Japan.
Nagata
T
T
Uchijima
M
M
Koide
Y
Y
D000818:Animals; D001427:Bacterial Toxins; D001428:Bacterial Vaccines / Q000276:immunology*; D019470:Biolistics; D015496:CD4-Positive T-Lymphocytes / Q000502:physiology*; D018984:Epitopes, T-Lymphocyte; D006183:H-2 Antigens / Q000502:physiology*; D006360:Heat-Shock Proteins / Q000235:genetics / Q000276:immunology*; D006460:Hemolysin Proteins; D007371:Interferon-gamma / Q000502:physiology*; D008089:Listeria monocytogenes / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014611:Vaccination; D019444:Vaccines, DNA / Q000276:immunology*
D001427:Bacterial Toxins; D001428:Bacterial Vaccines; D018984:Epitopes, T-Lymphocyte; D006183:H-2 Antigens; C024755:H-2K(K) antigen; D006360:Heat-Shock Proteins; D006460:Hemolysin Proteins; D019444:Vaccines, DNA; D007371:Interferon-gamma; C038705:hlyA protein, Listeria monocytogenes