title: Validation of a new experimental model of colon cancer.
abstract: BACKGROUND
  Most of the published animal studies that have evaluated tumor growth and port site metastases in laparoscopy have utilized a cell suspension model and thus cannot be compared to the clinical situation. Although solid tumor models have been developed, there has been no experimental model that establishes an orthotopic tumor in the rectum, reflecting the clinical situation of a solid colonic cancer.
  
  
  METHODS
  Tumor cells (colon adenocarcinoma DHD/K1/TRb) were administered intraperitoneally in rats, which were used as solid tumor donors. A 20-mg piece of solid tumor from the donor was placed in a submucosal blister created in the rectum wall of the study rats. The approach to the submucosal blister was made through the mucosa after contralateral enterotomy. In order to validate the model, this intervention was performed in 10 cases (group A). After 10 days of intervention, the rats were submitted to resection of the rectum and histological examination of the specimen. In another 10 rats (group B), manipulation of the tumor was performed after 10 days to cause tumor cell spillage. The likelihood of tumor dissemination was investigated in this group 20 days after this intervention.
  
  
  RESULTS
  Group A developed solid tumors in seven of 10 cases (70%). All of the tumors were localized between the muscular and the mucosal layer, with preservation of the serosa and without affecting the enterotomy. In all of the rats in group B, macroscopic tumor was observed in the upper rectum (100%) 10 days after its induction. Twenty days after tumor manipulation, nine rats had local tumor dissemination; two of them also had general tumor dissemination in the abdominal cavity.
  
  
  CONCLUSIONS
  We established a novel solid colonic tumor model in rats for the investigation of intraoperative tumor cell spillage during resection of the colon and the development of port site metastases.
authors.affiliation: Department of General Surgery, Clinic Hospital, University of Barcelona, C/Villarroel 170, 08036 Barcelona, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Balague
authors.forename: C
authors.initials: C
authors.lastname: Braumann
authors.forename: K
authors.initials: K
authors.lastname: Führer
authors.forename: H
authors.initials: H
authors.lastname: Guski
authors.forename: C A
authors.initials: CA
authors.lastname: Jacobi
mesh_terms: D000230:Adenocarcinoma / Q000473:pathology* / Q000556:secondary / Q000601:surgery*; D000818:Animals; D003110:Colonic Neoplasms / Q000473:pathology* / Q000601:surgery*; D004195:Disease Models, Animal; D007413:Intestinal Mucosa; D010535:Laparoscopy / Q000009:adverse effects*; D008297:Male; D009364:Neoplasm Recurrence, Local / Q000473:pathology; D009366:Neoplasm Seeding; D009368:Neoplasm Transplantation / Q000379:methods; D011677:Punctures / Q000009:adverse effects; D051381:Rats; D012007:Rectum; D014407:Tumor Cells, Cultured / Q000637:transplantation