title: Prognostic significance of free gastrointestinal tumor cells in peritoneal lavage detected by immunocytochemistry and polymerase chain reaction.
abstract: OBJECTIVE
  The aim of our study was to identify tumor cells in peritoneal lavage comparatively with immunocytochemistry (ICC) and half-nested reverse transcriptase-polymerase chain reaction (RT-PCR) using carcinoembryonic antigen (CEA) as marker and to evaluate their prognostic significance.
  
  
  METHODS
  In 75 patients who underwent surgery for a carcinoma of the colorectum (n=49), stomach (n=17) or pancreas (n=9) and 13 patients with an abdominal aortic aneurysm (control group) the abdomen was irrigated with saline solution immediately after laparotomy. Cells were separated by Ficoll-density centrifugation and divided into 2 equal volumes for ICC and RT-PCR. For ICC cells were spun onto slides by cytospin centrifugation and stained with a monoclonal antibody (mab) against CEA using the APAAP method. For RT-PCR total RNA was extracted from the cells, transcribed into cDNA and amplified with CEA-specific primers. Lavages of 13 patients with an abdominal aortic aneurysm and blood samples of 6 healthy donors served as controls.
  
  
  RESULTS
  Immunostained tumor cells were found in peritoneal lavage in 23% (17/75) of all patients, whereas 63% (47/75) of patients gave a positive result by RT-PCR analysis. In the control group (n=13) no patient presented with tumor cells in ICC, however 5 of 13 (38%) showed amplified CEA-mRNA by RT-PCR, and so did one of six blood samples. Using ICC technique, we found significant correlations between detection rates and pT-, pN-, pM-categories as well as tumor stage. On the contrary, by RT-PCR significant correlations were observed only between pT- and pM-categories and detection rates. Detection of tumor cells in peritoneal lavage with both techniques was associated with poor prognosis. Moreover, these tumor cells are an independent prognostic factor and may have an influence on the development of peritoneal carcinomatosis.
  
  
  CONCLUSIONS
  ICC is a useful method for detection of tumor cells in peritoneal lavage. In contrast, half-nested RT-PCR cannot be recommended, as the detection rates are unproportionally high, obviously as a result of CEA-mRNA expression in nontumor cells.
authors.affiliation: Surgical Research Laboratory, University Clinic of Lübeck, Ratzeburger Allee 160, 23538 Lübeck, Germany. broll@medinf.mu-luebeck.de
authors.forename: R
authors.initials: R
authors.lastname: Broll
authors.forename: M
authors.initials: M
authors.lastname: Weschta
authors.forename: U
authors.initials: U
authors.lastname: Windhoevel
authors.forename: S
authors.initials: S
authors.lastname: Berndt
authors.forename: O
authors.initials: O
authors.lastname: Schwandner
authors.forename: U
authors.initials: U
authors.lastname: Roblick
authors.forename: T H
authors.initials: TH
authors.lastname: Schiedeck
authors.forename: H
authors.initials: H
authors.lastname: Schimmelpenning
authors.forename: H P
authors.initials: HP
authors.lastname: Bruch
authors.forename: M
authors.initials: M
authors.lastname: Duchrow
mesh_terms: D000230:Adenocarcinoma / Q000601:surgery; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D017544:Aortic Aneurysm, Abdominal / Q000601:surgery; D001202:Ascitic Fluid / Q000473:pathology*; D002272:Carcinoembryonic Antigen / Q000097:blood; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D005260:Female; D005770:Gastrointestinal Neoplasms / Q000473:pathology* / Q000601:surgery; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D008875:Middle Aged; D010533:Peritoneal Lavage; D011379:Prognosis; D016016:Proportional Hazards Models; D011446:Prospective Studies; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
chemical_list: D002272:Carcinoembryonic Antigen