title: Comparative analysis of PAH:DNA adducts formed in lung of mice exposed to neat coal tar and soils contaminated with coal tar.
abstract: 7H-Benzo[c]fluorene (benzo[c]fluorene) is a major DNA adduct forming component of coal tar in lung of mice. The present study evaluated the types of PAH:DNA adducts formed from different neat coal tar samples and soils contaminated with coal tar. Mice were fed diets containing coal tar either neat or as a contaminant in an environmental soil sample for 14 days, and the types of chemical:DNA adducts formed in lung were evaluated using 32P-postlabeling and HPLC analysis. Three major DNA adducts derived respectively from benzo[b]fluoranthene (B[b]F), benzo[a]pyrene (B[a]P), and benzo[c]fluorene were detected in three of the four neat coal tar samples evaluated. In contrast, only a single major DNA adduct derived from benzo[c]fluorene was observed with the remaining tar sample. Ingestion of coal tar contaminated soil resulted in DNA adducts primarily derived from benzo[c]fluorene and B[b]F; a B[a]P derived DNA adduct was not detected. The DNA adducts derived from benzo[c]fluorene and B[b]F but not B[a]P were also observed with animals fed methylene chloride extracts of three of these soils but not the one designated A1000H soil. However, the extract of A1000H resulted in a B[a]P:DNA adduct being detected along with adducts formed from B[b]F and benzo[c]fluorene. The selective formation of the benzo[c]fluorene:DNA adduct with coal tar contaminated soils indicates that the in vivo systemic bioavailability and/or metabolism of benzo[c]fluorene is relatively high when compared to other DNA adducting hydrocarbons within coal tar. Benzo[c]fluorene may play a critical role in the potential of contaminated soil to induce a toxicological response in animals.
authors.affiliation: College of Pharmacy, Department of Pharmaceutical Chemistry, Rutgers, State University of New Jersey, 160 Frelinghuysen Road, Piscataway, New Jersey 08854-8020, USA.
authors.forename: A
authors.initials: A
authors.lastname: Koganti
authors.forename: R
authors.initials: R
authors.lastname: Singh
authors.forename: B L
authors.initials: BL
authors.lastname: Ma
authors.forename: E H
authors.initials: EH
authors.lastname: Weyand
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001682:Biological Availability; D002273:Carcinogens / Q000032:analysis*; D003031:Coal; D018736:DNA Adducts; D005260:Female; D005449:Fluorenes / Q000032:analysis*; D008168:Lung / Q000187:drug effects / Q000473:pathology; D051379:Mice; D011084:Polycyclic Aromatic Hydrocarbons / Q000009:adverse effects* / Q000493:pharmacokinetics; D012989:Soil Pollutants / Q000009:adverse effects* / Q000493:pharmacokinetics
chemical_list: D002273:Carcinogens; D003031:Coal; D018736:DNA Adducts; D005449:Fluorenes; D011084:Polycyclic Aromatic Hydrocarbons; D012989:Soil Pollutants; C041513:benzo(c)fluorene