[Effects of bile acid preparations on DNA biosynthesis, apoptosis, and necrosis in hepatocytes in vitro].
Using primary culture of hepatocytes it was shown that glycochenodeoxycholic acid (GCDCA) in a dose dependent manner induces apoptosis or necrosis of the hepatocytes and inhibits DNA biosynthesis. Ursodeoxycholic acid (UDCA) and tauroursodexycholic acid (TUDCA) do not cause apoptosis or necrosis of the hepatocytes in a range of doses 25-400 micrograms/ml. But in the presence of GCDCA both preparations have an antiapoptosogenic effect and TUDCA has also an antinecrosogenic effect.
Danchenko
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Vitebsk Medical University, 210602 Vitebsk, Frunze ave., 27. scidep@vitmed.belpak.vitebsk.by
D000818:Animals; D017209:Apoptosis; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis*; D005999:Glycochenodeoxycholic Acid / Q000494:pharmacology; D022781:Hepatocytes / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D009336:Necrosis; D051381:Rats; D017208:Rats, Wistar; D013655:Taurochenodeoxycholic Acid / Q000494:pharmacology*; D014580:Ursodeoxycholic Acid / Q000494:pharmacology*
D013655:Taurochenodeoxycholic Acid; C031655:ursodoxicoltaurine; D005999:Glycochenodeoxycholic Acid; D014580:Ursodeoxycholic Acid; D004247:DNA