title: [Effects of the alterations of membrane shear elastic modulus and viscosity on the deformation and orientation of RBCs].
abstract: Neuraminidase can partly remove the surface charge of RBCs through a biochemical interaction; thus it can give rise to alterations in the microstructure of membrane, the shear elastic modulus (E) and the viscosity(micron) of membrane. Changing the time of treatment and the dose of neuraminidase and using a new ektacytometry that can separate deformation index DI into orientation index (DI)or and small deformation index (DI)d for RBCs in shear flow field of low viscosity, we measured (DI)d and the half time t0.5 when the DI recovered to half of the maximum in the process of relaxation for every treated sample. (DI)d and t0.5 were put respectively into the RBC membrane shear elastic modulus formula and the membrane viscosity formula which were put forward by Wen Zong-yao and Yan Zong-yi et al[1]. The rules of the alterations of E and micron were obtained. We also measured DI and (DI)or. It was found that E and micron increased greatly but DI and (DI)or decreased when the dose of neuraminidase and the time of treatment were increased. There was a contrary correlation between them. These data demonstrated that the increase of E and micron weakened the deformability and the ability of orientation of RBCs.
authors.affiliation: Dept. of Medical Physics, Beijing Medical University, Beijing 100083.
authors.forename: L
authors.initials: L
authors.lastname: Xie
authors.forename: H
authors.initials: H
authors.lastname: Yang
authors.forename: W
authors.initials: W
authors.lastname: Yao
authors.forename: D
authors.initials: D
authors.lastname: Liu
authors.forename: Z
authors.initials: Z
authors.lastname: Zeng
authors.forename: W
authors.initials: W
authors.lastname: Ka
authors.forename: D
authors.initials: D
authors.lastname: Sun
authors.forename: Z
authors.initials: Z
authors.lastname: Wen
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004548:Elasticity; D004907:Erythrocyte Deformability; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000502:physiology*; D004912:Erythrocytes / Q000187:drug effects / Q000502:physiology*; D009439:Neuraminidase / Q000494:pharmacology; D011817:Rabbits; D014783:Viscosity
chemical_list: D009439:Neuraminidase