Pyrazinone conjugates as potential cephalosporin allergens.
Cephalosporins with an alpha-amino group on the 7-beta-acyl substituent have been shown to undergo aminolysis with subsequent formation of a fluorescent pyrazinone degradation product. An analogous reaction seems to take place upon in vitro conjugation of such cephalosporins to albumins and polylysine. A corresponding pyrazinone-polylysine conjugate was able to bind IgE antibodies in sera from patients with suspected allergy to beta-lactams. These findings may have applications in the diagnosis of allergy against aminocephalosporins.
Venemalm
L
L
Pharmacia Diagnostics AB, SE-751 82, Uppsala, Sweden. lennart.venemalm@pharmacia.com
D000418:Albumins / Q000737:chemistry*; D000485:Allergens / Q000737:chemistry* / Q000276:immunology; D001666:Binding Sites, Antibody; D002511:Cephalosporins / Q000737:chemistry*; D005453:Fluorescence; D006801:Humans; D006967:Hypersensitivity / Q000175:diagnosis; D007073:Immunoglobulin E / Q000097:blood / Q000737:chemistry*; D007769:Lactams / Q000737:chemistry; D011107:Polylysine / Q000737:chemistry*; D011719:Pyrazines / Q000737:chemistry* / Q000302:isolation & purification
D000418:Albumins; D000485:Allergens; D002511:Cephalosporins; D007769:Lactams; D011719:Pyrazines; D011107:Polylysine; D007073:Immunoglobulin E