title: Association of porcine circovirus 2 with reproductive failure in pigs: a retrospective study, 1995-1998.
abstract: In order to determine if vertically transmitted porcine circovirus (PCV) has played a role in reproductive failure in pigs in areas of endemic infection, archival fixed tissues were examined by polymerase chain reaction (PCR) and immunohistochemistry. Tissues tested were from routine cases of abortion or reproductive failure submitted between 1995 and 1998 to the diagnostic laboratory at the Western College of Veterinary Medicine, Saskatoon. They originated from 29 high-health herds in the provinces of Alberta and Saskatchewan and comprised a total of 36 individual submissions. Porcine circovirus type 1 (PCV1) was not detected by PCR in any submitted tissues. Porcine circovirus type 2 (PCV2) was not detected by PCR or immunohistochemistry in any of the submitted tissue. The effect of extended formalin fixation on the detection of PCV2 by PCR was assessed and fixation for up to one week had no gross effect on sensitivity of detection using this PCR technique. Failure to detect porcine circoviruses in cases of reproductive failure prior to 1999 in areas of endemic infections, suggests that reproductive disease may be a new clinical manifestation of PCV2 infection, and that vertical transmission may not have been the primary mechanism of initial dissemination of the virus in the pig population.
authors.affiliation: Department of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan S7N 5B4.
authors.forename: J
authors.initials: J
authors.lastname: Bogdan
authors.forename: K
authors.initials: K
authors.lastname: West
authors.forename: E
authors.initials: E
authors.lastname: Clark
authors.forename: C
authors.initials: C
authors.lastname: Konoby
authors.forename: D
authors.initials: D
authors.lastname: Haines
authors.forename: G
authors.initials: G
authors.lastname: Allan
authors.forename: F
authors.initials: F
authors.lastname: McNeilly
authors.forename: B
authors.initials: B
authors.lastname: Meehan
authors.forename: S
authors.initials: S
authors.lastname: Krakowka
authors.forename: J A
authors.initials: JA
authors.lastname: Ellis
mesh_terms: D000034:Abortion, Veterinary / Q000209:etiology* / Q000821:virology; D000818:Animals; D018173:Circoviridae Infections / Q000635:transmission / Q000662:veterinary*; D017925:Circovirus / Q000302:isolation & purification*; D005260:Female; D007150:Immunohistochemistry / Q000662:veterinary; D018445:Infectious Disease Transmission, Vertical / Q000662:veterinary*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012098:Reproduction; D012189:Retrospective Studies; D012680:Sensitivity and Specificity; D013552:Swine; D013553:Swine Diseases / Q000635:transmission / Q000821:virology*