Mitochondrial cytochrome oxidase immunolabeling in aged human temporal bones.
Presbycusis, an age-related hearing loss, is accompanied by histopathological cochlear changes including variable amounts of degeneration of the auditory receptors, neurons and the stria vascularis. The causes of degeneration are unknown, although acoustic trauma and exposure to ototoxic agents are certainly contributors to the cellular degeneration. Acquired mitochondrial DNA defects are postulated as important determinants of aging in neuromuscular tissues. The cochlear neurons are highly metabolic and are, therefore, likely to be affected by mitochondrial DNA defects. Sequence analysis has demonstrated a significant number of acquired mutations in the cytochrome oxidase gene in the neurons from aged human cochleas. The current study used immunohistochemical labeling of cytochrome oxidase in the neuronal cell bodies in archival celloidin sections to evaluate relationships among label density, hearing loss, number of neurons and mitochondrial DNA changes within individual cochleas. Label density was less in many aged temporal bones, but not all. There was no relationship among any other variables. It is concluded that while there may be a decrease in the amount of cytochrome oxidase expression in aged spiral ganglion cell bodies, there are many other factors that contribute to hearing loss and cellular degeneration.
Keithley
E M
EM
Department of Surgery, University of California, San Diego, La Jolla, 92093, USA. ekeithley@ucsd.edu
Harris
B
B
Desai
K
K
Linthicum
F
F
Fischel-Ghodsian
N
N
D000368:Aged; D000375:Aging / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D004272:DNA, Mitochondrial / Q000235:genetics; D003576:Electron Transport Complex IV / Q000378:metabolism*; D006801:Humans; D007231:Infant, Newborn; D008875:Middle Aged; D008928:Mitochondria / Q000201:enzymology; D009154:Mutation; D010040:Otosclerosis / Q000201:enzymology / Q000235:genetics / Q000473:pathology; D011304:Presbycusis / Q000201:enzymology / Q000235:genetics / Q000473:pathology; D013701:Temporal Bone / Q000201:enzymology* / Q000473:pathology
D004272:DNA, Mitochondrial; D003576:Electron Transport Complex IV