title: Inhibition of prostaglandin E2 production by platycodin D isolated from the root of Platycodon grandiflorum.
abstract: Platycodin D, isolated from the root of Platycodon grandiflorum A. DC. (Campanulaceae) suppressed prostaglandin E2 production at 10 and 30 microM in rat peritoneal macrophages stimulated by the protein kinase C activator 12-O-tetradecanoylphorbol 13-acetate (TPA). Platycodin D3 and oleanolic acid showed no effect at these concentrations. Western blot analysis revealed that the induction of COX-2 protein by TPA was inhibited by platycodin D in parallel with the inhibition of prostaglandin E2 production. Platycodin D showed no direct effect on COX-1 and COX-2 activities. TPA-induced release of [3H]arachidonic acid from pre-labeled macrophages was also not inhibited by platycodin D.
authors.forename: Y P
authors.initials: YP
authors.lastname: Kim
authors.forename: E B
authors.initials: EB
authors.lastname: Lee
authors.forename: S Y
authors.initials: SY
authors.lastname: Kim
authors.forename: D
authors.initials: D
authors.lastname: Li
authors.forename: H S
authors.initials: HS
authors.lastname: Ban
authors.forename: S S
authors.initials: SS
authors.lastname: Lim
authors.forename: K H
authors.initials: KH
authors.lastname: Shin
authors.forename: K
authors.initials: K
authors.lastname: Ohuchi
mesh_terms: D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism; D002478:Cells, Cultured / Q000187:drug effects* / Q000378:metabolism; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2; D003907:Dexamethasone / Q000494:pharmacology; D015232:Dinoprostone / Q000032:analysis / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D004790:Enzyme Induction / Q000187:drug effects; D007213:Indomethacin / Q000494:pharmacology; D007527:Isoenzymes / Q000378:metabolism; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000378:metabolism; D008565:Membrane Proteins; D009828:Oleanolic Acid / Q000737:chemistry / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry*; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism; D011493:Protein Kinase C / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014315:Triterpenes
chemical_list: D004365:Drugs, Chinese Herbal; D007527:Isoenzymes; D008565:Membrane Proteins; D012503:Saponins; D014315:Triterpenes; C431483:platycodin D3; D016718:Arachidonic Acid; D009828:Oleanolic Acid; D003907:Dexamethasone; C108953:platycodin D; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2; D011451:Prostaglandin-Endoperoxide Synthases; C496513:Ptgs1 protein, rat; D011493:Protein Kinase C; D015232:Dinoprostone; D013755:Tetradecanoylphorbol Acetate; D007213:Indomethacin