In vitro comparison of bioabsorbable and non-resorbable membranes in bone regeneration.
BACKGROUND
Barrier membranes are used to prevent down-growth of the oral mucosa along the root surface and to allow alveolar bone regeneration in guided tissue regeneration. Several studies have demonstrated bone regenerates in the presence of bioabsorbable and non-resorbable membranes, but no studies have compared multiple bioabsorbable barriers to one another and to non-resorbable barriers. This study evaluated the in vitro influence of bioabsorbable and non-resorbable membranes on specific parameters of human osteoblast activity.


METHODS
Human osteoblasts were cultured on bioabsorbable membranes made of collagen, hyaluronic acid, and poly DL-lactide, and the most common non-resorbable membrane which is made of expanded polytetrafluoroethylene (ePTFE). The osteoblasts were cultured in vitro for 24 hours on barrier membranes in the presence of 3H-thymidine and 3H-proline to study cell proliferation and collagen synthesis. Transforming growth factor-beta1 (TGF-beta1) secretion was evaluated in conditioned media using an ELISA kit.


RESULTS
The results showed that collagen and poly DL-lactide stimulated DNA synthesis more than ePTFE and hyaluronic acid. All bioabsorbable membranes significantly increased collagen synthesis and alkaline phosphatase activity. Collagen and hyaluronic acid increased secretion of TGF-beta1, a growth factor involved in bone remodeling.


CONCLUSIONS
These data suggest bioabsorbable membranes, particularly collagen and hyaluronic acid, may promote bone regeneration through their activity on osteoblasts.
Marinucci
L
L
Department of Experimental Medicine and Biochemistry, University of Perugia, Italy.
Lilli
C
C
Baroni
T
T
Becchetti
E
E
Belcastro
S
S
Balducci
C
C
Locci
P
P
D020341:Absorbable Implants; D000469:Alkaline Phosphatase / Q000378:metabolism; D001672:Biocompatible Materials / Q000737:chemistry; D001861:Bone Regeneration / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis / Q000737:chemistry; D017077:Culture Media, Conditioned; D004247:DNA / Q000096:biosynthesis; D004797:Enzyme-Linked Immunosorbent Assay; D048091:Guided Tissue Regeneration / Q000295:instrumentation*; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry; D008422:Materials Testing; D008567:Membranes, Artificial; D010006:Osteoblasts / Q000378:metabolism / Q000502:physiology; D011091:Polyesters / Q000737:chemistry; D011138:Polytetrafluoroethylene / Q000737:chemistry; D011392:Proline / Q000378:metabolism; D019275:Radiopharmaceuticals; D013223:Statistics as Topic; D013936:Thymidine / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000378:metabolism; D014316:Tritium
D001672:Biocompatible Materials; D017077:Culture Media, Conditioned; D008567:Membranes, Artificial; D011091:Polyesters; D019275:Radiopharmaceuticals; D016212:Transforming Growth Factor beta; D014316:Tritium; C033616:poly(lactide); D011138:Polytetrafluoroethylene; D006820:Hyaluronic Acid; D003094:Collagen; D004247:DNA; D011392:Proline; D000469:Alkaline Phosphatase; D013936:Thymidine