Clay-enhanced DNA separation in low-molecular-weight poly(N,N-dimethylacrylamide) solution by capillary electrophoresis.
The effect of the separation medium in capillary electrophoresis consisting of a low-molecular-mass poly(N,N-dimethylacrylamide) (PDMA) solution on the DNA separation by adding a small amount of montmorillonite clay into the polymer matrix is presented. On the separation of the pBR322/HaeIII digest, both the resolution and the efficiency were increased by adding 2.5-5.0 x 10(-5) g/mL clay into the 5% w/v PDMA with a molecular mass of only 100 K. Moreover, there was no increase in the migration time of DNA fragments. Similar results were observed by using a C-terminated pGEM-3Zf(+) sequencing DNA sample in a sequencing buffer. Experimental data also showed that the addition of clay increased the viscosity of the polymer solution. We attribute this effect to the structural change of the polymer matrix caused by the exfoliated clay sheets, whereby the thin clay sheets function like a "dynamic cross-linking plate" for the PDMA chains and effectively increase the apparent molecular mass of PDMA.
Liang
D
D
Department of Chemistry, SUNY at Stony Brook, 11794-3400, USA.
Song
L
L
Chen
Z
Z
Chu
B
B
D000178:Acrylamides / Q000737:chemistry; D001546:Bentonite; D004247:DNA / Q000737:chemistry / Q000302:isolation & purification*; D019075:Electrophoresis, Capillary / Q000379:methods*; D008970:Molecular Weight; D017422:Sequence Analysis, DNA / Q000379:methods; D012996:Solutions; D014783:Viscosity
D000178:Acrylamides; D012996:Solutions; C429790:poly(N,N-dimethylacrylamide); D001546:Bentonite; D004247:DNA