title: Determination of organophosphate and carbamate pesticides in spiked samples of tap water and fruit juices by a biosensor with photothermal detection.
abstract: The determination of organophosphate (paraoxon, chlorpyrifos, diazinon) and carbamate (carbaryl, carbofuran) pesticides in spiked drinking water and fruit juices was carried out using a photothermal biosensor. The biosensor consists of a cartridge containing immobilised enzyme acetylcholinesterase (AChE) placed in a flow-injection analysis (FIA) manifold and a photothermal detector based on thermal lens spectrometry. With this approach, 0.2 ng/ml of paraoxon can be detected in less than 15 min. Limits of detection for other organophosphate (chlorpyrifos, diazinon) and carbamate (carbaryl, carbofuran) pesticides varied, depending on their antiacetylcholinesterase (AntiAChE) toxicity, from 1 ng/ml to 4 microg/ml. The biosensor was used for the direct detection of pesticides in spiked tap water and fruit juices without any pretreatment steps. In these cases, the LOD3sigma of 1.5, 2.8 and 4 ng/ml paraoxon in tap water, orange juice and apple juice were obtained, respectively.
authors.affiliation: Nova Gorica Politechnic, Slovenia.
authors.forename: L
authors.initials: L
authors.lastname: Pogacnik
authors.forename: M
authors.initials: M
authors.lastname: Franko
mesh_terms: D001628:Beverages / Q000032:analysis; D015374:Biosensing Techniques / Q000295:instrumentation*; D002219:Carbamates; D017022:Flow Injection Analysis / Q000295:instrumentation; D005504:Food Analysis / Q000295:instrumentation; D005506:Food Contamination / Q000032:analysis; D005638:Fruit / Q000737:chemistry*; D007306:Insecticides / Q000032:analysis*; D009943:Organophosphorus Compounds; D010777:Photochemistry; D014874:Water Pollutants, Chemical / Q000032:analysis*; D014881:Water Supply / Q000032:analysis*
chemical_list: D002219:Carbamates; D007306:Insecticides; D009943:Organophosphorus Compounds; D014874:Water Pollutants, Chemical