Determination of enantiomer separation factors by nuclear magnetic resonance spectroscopy and by chiral liquid chromatography.
The equilibrium constants K+ and K- for formation of the diastereomeric complexes of the two enantiomers of O,O'-dibenzoyltartaric acid (DBTA) with the chiral selector N,N'-diallyltartardiamide bis-(4-tert.-butylbenzoate) (TBB) have been determined by 1H-NMR. The experiments were performed at different temperatures in CDCl3 or in cyclohexane-d12/2-propanol-d8 mixtures. The equilibrium constants from the 1H-NMR results have been compared with the retention factors (k') obtained from the chromatographic resolution of rac. DBTA on a Kromasil CHI-TBB column with the same solvents as mobile phases. A satisfactory correlation between the 1H-NMR data and the chromatographic data was found.
Skogsberg
U
U
Department of Chemistry, Göteborg University, Sweden.
Allenmark
S
S
D002853:Chromatography, Liquid / Q000379:methods*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D013237:Stereoisomerism