title: Resistance of a novel root canal sealer to bacterial ingress in vitro.
abstract: A dentin-bonding root canal sealer (ZUT) has been developed, consisting of an experimental glass ionomer cement (KT-308) and an antimicrobial silver-containing zeolite (0.2% by weight). This in vitro study evaluated the ability of ZUT used with or without gutta-percha, to resist bacterial ingress of Enterococcus faecalis over a period of 90 days. Canals of 80 single-rooted teeth were prepared with apical patency and filled as follows (n = 10): KT-308 alone; KT-308 with a single gutta-percha cone (SCGP); ZUT alone; ZUT with SCGP; AH26 alone; AH26 with SCGP; positive control-no root canal filling; and negative control-no root canal filling, with the apices of this group sealed with C&B Metabond cement. Teeth were coated with nail polish except for the apical 2 mm, and each tooth was sealed in a 4-ml glass vial, with an 18-gauge needle inserted through the vial cover and bonded into the pulp chamber with C&B Metabond cement. After sterilization with 2.5 Mrad gamma-radiation, Brain Heart Infusion broth with phenol red was injected into each vial. An inoculum of E. faecalis was pipetted through the needle into the pulp chamber every 5 days, and the broth was monitored daily for color change and turbidity. When change occurred, the broth was cultured for growth of E. faecalis. Kaplan-Meier survival analysis and the log-rank test revealed no significant differences among the three sealers used. The presence of gutta-percha, however, significantly improved resistance to bacterial ingress through obturated root canals (X, p < 0.05). Under the conditions of this study, the hypothesized advantage of ZUT (0.2% zeolite) was not demonstrated.
authors.affiliation: Endodontics Department, University of Toronto, Ontario, Canada.
authors.forename: N
authors.initials: N
authors.lastname: Padachey
authors.forename: V
authors.initials: V
authors.lastname: Patel
authors.forename: P
authors.initials: P
authors.lastname: Santerre
authors.forename: D
authors.initials: D
authors.lastname: Cvitkovitch
authors.forename: H P
authors.initials: HP
authors.lastname: Lawrence
authors.forename: S
authors.initials: S
authors.lastname: Friedman
mesh_terms: D000891:Anti-Infective Agents, Local / Q000627:therapeutic use*; D001419:Bacteria / Q000187:drug effects*; D001729:Bismuth / Q000627:therapeutic use; D001896:Boron Compounds / Q000627:therapeutic use; D016009:Chi-Square Distribution; D004396:Coloring Agents; D003786:Dental Pulp Cavity / Q000187:drug effects / Q000382:microbiology*; D017220:Dentin-Bonding Agents / Q000627:therapeutic use; D004338:Drug Combinations; D013293:Enterococcus faecalis / Q000187:drug effects / Q000254:growth & development; D004853:Epoxy Resins; D005720:Gamma Rays; D005899:Glass Ionomer Cements / Q000627:therapeutic use; D006171:Gutta-Percha / Q000627:therapeutic use; D006801:Humans; D016014:Linear Models; D008689:Methacrylates / Q000627:therapeutic use; D008709:Methenamine / Q000627:therapeutic use; D008768:Methylmethacrylates / Q000627:therapeutic use; D010637:Phenolsulfonphthalein; D012387:Root Canal Filling Materials / Q000627:therapeutic use*; D018915:Root Canal Preparation; D012834:Silver / Q000627:therapeutic use; D013223:Statistics as Topic; D013242:Sterilization; D016019:Survival Analysis; D013997:Time Factors; D014025:Titanium / Q000627:therapeutic use; D017641:Zeolites / Q000627:therapeutic use
chemical_list: D000891:Anti-Infective Agents, Local; D001896:Boron Compounds; D004396:Coloring Agents; D017220:Dentin-Bonding Agents; D004338:Drug Combinations; D004853:Epoxy Resins; D005899:Glass Ionomer Cements; D008689:Methacrylates; D008768:Methylmethacrylates; D012387:Root Canal Filling Materials; D017641:Zeolites; D012834:Silver; C028044:epoxy resin AH-26; D006171:Gutta-Percha; C049495:Super-bond; D014025:Titanium; D010637:Phenolsulfonphthalein; D008709:Methenamine; D001729:Bismuth