Isolation of a novel thermolabile heterodimeric ribonuclease with antifungal and antiproliferative activities from roots of the sanchi ginseng Panax notoginseng.
An isolation procedure, consisting of ion exchange chromatography on CM-Sepharose, affinity chromatography on Affi-gel blue gel, and fast protein liquid chromatography on Mono S, was utilized to purify a base-nonspecific, heterodimeric ribonuclease (RNase) with diverse activities from roots of the sanchi ginseng Panax notoginseng. The RNase is unique in that it consists of two different nonglycoprotein subunits with a molecular weight of 27 and 29 kDa, respectively. The latter subunit is characterized by an N-terminal sequence showing remarkable similarity to that of the bitter gourd RNase. The Panax notoginseng RNase demonstrates potent RNase and translation-inhibitory activities. In addition, it exhibits antiproliferative activity toward leukemia L1210 cells and antifungal activity against Physalospora piricola and Coprinus comatus. Its RNase activity is not heat-resistant, unlike most RNases which are thermostable.
Lam
S K
SK
Department of Biochemistry, Chinese University of Hong Kong, Shatin, Hong Kong, China. biochemistry@cuhk.edu.hk
Ng
T B
TB
D000818:Animals; D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects*; D002681:China; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D002853:Chromatography, Liquid; D003302:Coprinus / Q000187:drug effects / Q000254:growth & development; D019281:Dimerization; D004795:Enzyme Stability; D006358:Hot Temperature; D007700:Kinetics; D007939:Leukemia L1210; D051379:Mice; D008826:Microbial Sensitivity Tests; D008970:Molecular Weight; D005894:Panax / Q000201:enzymology*; D018517:Plant Roots / Q000201:enzymology; D010946:Plants, Medicinal; D014176:Protein Biosynthesis / Q000187:drug effects; D021122:Protein Subunits; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism; D012260:Ribonucleases / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D017386:Sequence Homology, Amino Acid; D013816:Thermodynamics; D014407:Tumor Cells, Cultured
D000935:Antifungal Agents; D021122:Protein Subunits; D012260:Ribonucleases