Genetic variation in Indian populations of Scirpophaga incertulas as revealed by RAPD-PCR analysis.
Scirpophaga incertulas, commonly referred to as yellow stem borer, is a predominant pest of rice causing serious losses in its yield. Genetic variation among populations of Scirpophaga incertulas collected from 28 hotspot locations in India was examined using the randomly amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR). In all, 32 primers were used and 354 amplification products were observed. No RAPD-PCR bands diagnostic to the pest population from any specific region were identified. Cluster analysis using UPGMA showed that, with the exception of the pest population from Pattambi, all the populations cluster as one group with GD values in the range of 6-22%, suggesting that gene flow between populations is independent of geographic distance and appears to be unrestricted. The relatively high GD value of 48% exhibited by the pest population from Pattambi was the only exception.
Kumar
L S
LS
Plant Molecular Biology Unit, Division of Biochemical Sciences, National Chemical Laboratory, Pune 411008, India. lalitha@dalton.ncl.res.in
Sawant
A S
AS
Gupta
V S
VS
Ranjekar
P K
PK
D000818:Animals; D004247:DNA / Q000032:analysis*; D004587:Electrophoresis, Agar Gel; D017344:Genes, Insect; D014644:Genetic Variation; D007194:India; D007915:Lepidoptera / Q000235:genetics*; D012275:Oryza / Q000469:parasitology; D016133:Polymerase Chain Reaction; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*; D015203:Reproducibility of Results
D004247:DNA