Isolation and characterization of Mn(III) tartrate from Phanerochaete chrysosporium culture broth.
High initial Mn(II) concentration results in accumulation of a Mn(III) tartrate complex in the growth medium of Phanerochaete chrysosporium. Since Mn(III) is the major oxidant in ligninolysis by manganese peroxidase, the role of accumulated complex should not be neglected when degradation experiments by a crude culture filtrate are performed. To study the Mn(III) complex oxidative potential it was isolated by absorption to polyamide followed by desorption with an alkaline methanol solution. High performance liquid chromatography analysis and atomic absorption spectroscopy confirmed that the isolate was Mn(III) tartrate. Oxidation of 2,2'-azino-bis(3-ethylbenz-thiazoline-6-sulfonate) was used for testing the temperature and pH stability of the isolate that also intensively oxidized 2,6-dimethoxyphenol. In comparison with the non-isolated complex in the culture filtrate, the isolate showed increased temperature and pH stability. The oxidative potential of the isolated Mn(III) tartrate was additionally tested by decolorization of the synthetic dye Indigo carmine.
Podgornik
H
H
University of Ljubljana, Faculty of Chemistry and Chemical Technology, Slovenia. helena.podgornik@uni-lj.si
Stegu
M
M
Podgornik
A
A
Perdih
A
A
D052160:Benzothiazoles; D002851:Chromatography, High Pressure Liquid; D017077:Culture Media, Conditioned / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D010084:Oxidation-Reduction; D010544:Peroxidases / Q000378:metabolism; D020075:Phanerochaete / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D013451:Sulfonic Acids / Q000378:metabolism; D013644:Tartrates / Q000737:chemistry* / Q000302:isolation & purification*; D013696:Temperature; D014462:Ultrafiltration
D052160:Benzothiazoles; D017077:Culture Media, Conditioned; D013451:Sulfonic Acids; D013644:Tartrates; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D010544:Peroxidases; C051129:manganese peroxidase; C029768:tartaric acid