Suppression of matrix metalloproteinase-2 and -9 mediated invasiveness by a novel matrix metalloproteinase inhibitor, BE16627B.
Cell invasion is a nature of malignant gliomas, demeriting to many efforts of the treatment. Matrix metalloproteinase (MMP) is acknowledged as a key factor in this complicated process. The aim of this study was to investigate whether inhibition of MMP activity in malignant glioma cells could be achieved by a novel agent, BE16627B (BE). Malignant glioma cell lines, U87MG, U251MG, and U373MG, were employed to evaluate inhibitory effect on zymogram, type IV collagenolysis assay, and haptoinvasion assay for 24 h exposure of BE, following preliminar
Watanabe
K
K
Department of Neurosurgery, Nippon Medical School, Tokyo, Japan.
Yoshida
D
D
Noha
M
M
Teramoto
A
A
D000970:Antineoplastic Agents / Q000494:pharmacology*; D024141:Collagen Type IV / Q000378:metabolism; D004396:Coloring Agents; D004151:Dipeptides / Q000494:pharmacology*; D005910:Glioma / Q000473:pathology* / Q000503:physiopathology*; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000502:physiology*; D020780:Matrix Metalloproteinase 9 / Q000502:physiology*; D009361:Neoplasm Invasiveness; D011480:Protease Inhibitors / Q000494:pharmacology*; D013386:Succinates / Q000494:pharmacology*; D013778:Tetrazolium Salts; D013844:Thiazoles; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D024141:Collagen Type IV; D004396:Coloring Agents; D004151:Dipeptides; D011480:Protease Inhibitors; D013386:Succinates; D013778:Tetrazolium Salts; D013844:Thiazoles; C085667:L-N-(N-hydroxy-2-isobutylsuccinamoyl)seryl-L-valine; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; C022616:thiazolyl blue