title: Structural and functional characterization of B-domain deleted recombinant factor VIII.
abstract: A new high-purity recombinant factor VIII preparation has been developed for the treatment of hemophilia A. Structurally, this factor VIII preparation, B-domain deleted recombinant factor VIII (BDDrFVIII), differs from other recombinant and plasma-derived factor VIII preparations in that most of the B-domain has been deleted. To ensure that BDDrFVIII contains the requisite structural and functional features, it has been subjected to detailed biochemical and biophysical characterization in comparison to the plasma-derived form of factor VIII. Laboratory studies have shown that the primary, secondary, and tertiary structures of BDDrFVIII and the posttranslational modifications are similar to those of the [80 + 90]-kd form of plasma-derived factor VIII. In addition, BDDrFVIII has full biologic activity compared with full-length factor VIII preparations.
authors.affiliation: Pharmacia AB, Stockholm, Sweden.
authors.forename: H
authors.initials: H
authors.lastname: Sandberg
authors.forename: A
authors.initials: A
authors.lastname: Almstedt
authors.forename: J
authors.initials: J
authors.lastname: Brandt
authors.forename: V M
authors.initials: VM
authors.lastname: Castro
authors.forename: E
authors.initials: E
authors.lastname: Gray
authors.forename: L
authors.initials: L
authors.lastname: Holmquist
authors.forename: M
authors.initials: M
authors.lastname: Lewin
authors.forename: U
authors.initials: U
authors.lastname: Oswaldsson
authors.forename: M
authors.initials: M
authors.lastname: Mikaelsson
authors.forename: M A
authors.initials: MA
authors.lastname: Jankowski
authors.forename: M
authors.initials: M
authors.lastname: Bond
authors.forename: H A
authors.initials: HA
authors.lastname: Scoble
mesh_terms: D000595:Amino Acid Sequence; D004347:Drug Interactions; D005169:Factor VIII / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D011485:Protein Binding
chemical_list: C427184:recombinant factor VIII SQ; D005169:Factor VIII