Assignment of D-amino-acid oxidase gene to a human and a mouse chromosome.
A part of D-amino-acid oxidase gene was amplified in the human and mouse by polymerase chain reaction. The amplified fragments were ligated to plasmids and then cloned. The plasmids containing the parts of D-amino-acid oxidase gene were biotinylated and hybridized to human and mouse metaphase chromosomes. The chromosomal slides were treated with fluorescein isothiocyanate (FITC)-conjugated avidin. The hybridized signals were amplified with biotinylated anti-avidin antibody and FITC-avidin. The chromosomes were counter-stained with diamidino-phenylindole for assignment of the signal to a specific band. Using this fluorescence in situ hybridization (FISH), D-amino-acid oxidase gene was assigned to human chromosome 12q23-24.1 and mouse chromosome 5E3-F. Since these regions are syntenic between human and mouse, the present results indicate that the locus for this enzyme has been conserved through evolution.
Konno
R
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Department of Microbiology, Dokkyo University School of Medicine, Mibu, Tochigi, Japan.
D000818:Animals; D002874:Chromosome Mapping; D002881:Chromosomes, Human, Pair 12; D003001:Cloning, Molecular; D003605:D-Amino-Acid Oxidase / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D008856:Microscopy, Fluorescence; D008957:Models, Genetic; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction
C417983:DAO protein, human; C485958:Dao1 protein, mouse; D003605:D-Amino-Acid Oxidase