Carboxyl-terminal residues of mammalian fibrinogen and fibrin.
The carboxyl-terminal residues of mammalian fibrinogens of six different species and the chain peptides, alpha(A), beta(B) and gamma, isolated from these fibrinogens were determined by hydrazinolysis, digestion with carboxypeptidases and selective tritium labelling. The C-terminal ends of bovine fibrinogen and fibrin were identified as proline and valine, in the molar ratio of approximately 1:2. Proline was identified as the C-terminus of the alpha(A)-chain, and C-terminal valine was found on both the beta(B)- and gamma-chains. On hydrazinolysis after selective tritium labelling of fibrinogen, radioactive C-terminal valine was also identified. The same C-terminal ends as those of bovine fibrinogen were found on the corresponding chain peptides isolated from sheep fibrinogen. The C-terminal residues of all the chain peptides of human and horse fibrinogens, however, were valine. In hog and dog fibrinogens, proline was identified at the C-termini of the alpha(A)-chains, and C-terminal valine and isoleucine were found on the beta(B)- and gamma-chains, respectively. Thus, the C-terminal amino acid residues of the fibrinogens of all mammalian species tested were very similar. It should be noted that hydrophobic amino acids, like isoleucine, valine and proline, are mainly located in the C-terminal ends of all three chain peptides in the fibrinogen molecule.
Okude
M
M
Division of Plasma Proteins, Institute for Protein Research, Isaka University, Joancho 36, Kita-ku, Osaka, Japan.
Iwanaga
S
S
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002417:Cattle; D004285:Dogs; D005337:Fibrin / Q000737:chemistry*; D005340:Fibrinogen / Q000737:chemistry*; D006736:Horses; D006801:Humans; D007202:Indicators and Reagents; D008322:Mammals; D010446:Peptide Fragments / Q000737:chemistry*; D011392:Proline / Q000032:analysis; D012756:Sheep; D013552:Swine; D014633:Valine / Q000032:analysis
D000596:Amino Acids; D007202:Indicators and Reagents; D010446:Peptide Fragments; D005337:Fibrin; D005340:Fibrinogen; D011392:Proline; D014633:Valine