Detection of Mycoplasma bovis in preservative-treated field milk samples.
Control of mycoplasmal mastitis requires individual cow milk sampling for culture and identification of Mycoplasma bovis. This sampling is time-consuming and expensive. Currently, some herds sample cows monthly with the dairy herd improvement (DHI) program, but a preservative is added to this milk that kills M. bovis. In this paper, a nested polymerase chain reaction (PCR) procedure that allows for rapid testing of preservative-treated milk is validated. The specificity of the nested PCR assay was confirmed by testing isolated nucleic acids of other organisms phylogenetically related to M. bovis or common to milk. A comparison against blind-passage culture on 53 field milk samples determined its sensitivity. Exposure of seeded milk samples to the procedure resulted in a sensitivity of 5.1 cfu equivalents per milliliter. Analysis of these results proved that the nested PCR assay was as sensitive as traditional culture and can be used on preservative-treated milk.
Pinnow
C C
CC
Microbiology Research and Development, Hospital Products Division, Abbott Laboratories, Abbott Park, IL 60064, USA.
Butler
J A
JA
Sachse
K
K
Hotzel
H
H
Timms
L L
LL
Rosenbusch
R F
RF
D000818:Animals; D002417:Cattle; D004269:DNA, Bacterial / Q000032:analysis; D005260:Female; D005520:Food Preservatives / Q000008:administration & dosage*; D008414:Mastitis, Bovine / Q000175:diagnosis* / Q000382:microbiology; D008892:Milk / Q000382:microbiology*; D009174:Mycoplasma / Q000302:isolation & purification*; D009175:Mycoplasma Infections / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D004269:DNA, Bacterial; D005520:Food Preservatives