title: Contractile responses in arteries subjected to hypertensive pressure in seven-day organ culture.
abstract: Early stage changes in hypertensive arteries have a significant effect on the long-term adaptation of the arteries. Compared to the long-term adaptation, little is known about the early dimensional and functional changes in hypertensive arteries in the first few days of hypertension. To study the early stage changes in hypertensive arteries, porcine common carotid arteries were cultured for seven days in a simplified ex vivo artery organ culture system with pulsatile flow under hypertensive (200+/-30 mm Hg) or normotensive (100+/-20 mm Hg) pressure conditions while maintaining a physiological mean wall shear stress of 15 dyn/cm2. Vessel viability was demonstrated by contractile diameter responses to norepinephrine (NE), carbachol (CCh), and sodium nitroprusside (SNP) as well as staining for mitochondrial activity and cell apoptosis/necrosis. The results show that arteries demonstrated strong contractile responses to NE, CCh, and SNP, basal tone, and viable mitochondria in the organ culture system for seven days. Hypertensive arteries demonstrated a stronger contractile response than normotensive arteries (p<0.05). Diameter enlargement was observed in hypertensive arteries as compared to arteries cultured under normotensive conditions. In conclusion, the pulsatile culture system can maintain arteries viable with active vasomotion tone for up to seven days. Hypertensive pressure causes arterial adaptation by significantly increasing arterial diameter and contractile response within the first seven days.
authors.affiliation: School of Mechanical Engineering, Georgia Institute of Technology, Atlanta 30332-0405, USA. hhan@sununo.me.gatech.edu
authors.forename: H C
authors.initials: HC
authors.lastname: Han
authors.forename: D N
authors.initials: DN
authors.lastname: Ku
mesh_terms: D000222:Adaptation, Physiological; D001698:Biomedical Engineering; D002217:Carbachol / Q000494:pharmacology; D017536:Carotid Artery, Common / Q000187:drug effects / Q000473:pathology / Q000503:physiopathology*; D006973:Hypertension / Q000473:pathology / Q000503:physiopathology*; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000473:pathology / Q000503:physiopathology; D009599:Nitroprusside / Q000494:pharmacology; D009638:Norepinephrine / Q000494:pharmacology; D009924:Organ Culture Techniques; D010477:Perfusion; D011673:Pulsatile Flow; D013997:Time Factors; D014661:Vasoconstriction / Q000187:drug effects / Q000502:physiology*
chemical_list: D009599:Nitroprusside; D002217:Carbachol; D009638:Norepinephrine