title: Contribution to the development of the stapedius muscle structure in human fetuses.
abstract: The purpose of this report is to contribute to the knowledge of the morphogenesis of the human stapedius muscle. We have studied the stapedius structure development in 17 temporal bones from human fetuses. Chronological ages were from 48 days (21 mm) to 29 weeks (270 mm). Samples were fixed in 10% formol, decalcified with 2% nitric acid, embedded in Paraplast, sectioned in sequence of 7 microns and were stained with haematoxylin & eosin and Martins' trichrome. Four maturation stages were observed in the development of the stapedius muscle structure. First, the formation of the 'primordium muscularis' in the mesenchyme of the tympanic cavity (promyoblasts) was observed in a 36-mm embryo. Second, its differentiation into myoblasts, and its next cellular fusion to form muscle tubes, was seen in a 99-mm foetus. In the third stage, myofibrogenesis phenomena were developed, to synthesize, the myotube myoblasts, myofilaments, phenomena we observed that took place during the 14th to 17th weeks of development. The last stage was characterized by the presence of striated muscle fibres, at first intermingled with myotubes (18th to 26th weeks), until in the 29th week fetuses (270 mm) the skeletal muscle fibre was completely differentiated. We conclude that in the 29th week of development (270 mm) the stapedius muscle structure is completely differentiated, although the functional development is not complete until birth.
authors.affiliation: Department of Morphology, School of Medicine, University of Zaragoza, Spain. jwhyte@posta.unizar.es
authors.forename: J
authors.initials: J
authors.lastname: Whyte
authors.forename: A
authors.initials: A
authors.lastname: Cisneros
authors.forename: C
authors.initials: C
authors.lastname: Yus
authors.forename: J
authors.initials: J
authors.lastname: Blasco
authors.forename: A
authors.initials: A
authors.lastname: Torres
authors.forename: R
authors.initials: R
authors.lastname: Sarrat
mesh_terms: D005314:Embryonic and Fetal Development; D005260:Female; D006801:Humans; D011247:Pregnancy; D013198:Stapedius / Q000196:embryology*