Identification of vaccine candidate antigens from a genomic analysis of Porphyromonas gingivalis.
Porphyromonas gingivalis is a key periodontal pathogen which has been implicated in the etiology of chronic adult periodontitis. Our aim was to develop a protein based vaccine for the prevention and or treatment of this disease. We used a whole genome sequencing approach to identify potential vaccine candidates. From a genomic sequence, we selected 120 genes using a series of bioinformatics methods. The selected genes were cloned for expression in Escherichia coli and screened with P. gingivalis antisera before purification and testing in an animal model. Two of these recombinant proteins (PG32 and PG33) demonstrated significant protection in the animal model, while a number were reactive with various antisera. This process allows the rapid identification of vaccine candidates from genomic data.
Ross
B C
BC
Research and Development, CSL Ltd., 45 Poplar Road, Vic. 3052, Parkville, Australia.
Czajkowski
L
L
Hocking
D
D
Margetts
M
M
Webb
E
E
Rothel
L
L
Patterson
M
M
Agius
C
C
Camuglia
S
S
Reynolds
E
E
Littlejohn
T
T
Gaeta
B
B
Ng
A
A
Kuczek
E S
ES
Mattick
J S
JS
Gearing
D
D
Barr
I G
IG
D000595:Amino Acid Sequence; D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology*; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology; D001428:Bacterial Vaccines / Q000276:immunology*; D015153:Blotting, Western; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D016966:Porphyromonas gingivalis / Q000235:genetics / Q000276:immunology*; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley; D014614:Vaccines, Synthetic / Q000276:immunology*
D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D014614:Vaccines, Synthetic