Sequence analysis of candidate genes for common susceptibility to type 1 and type 2 diabetes in mice.
Although type 1 and type 2 diabetes are regarded as clinically distinct diseases, several lines of evidence have suggested common genetic factors between the two types of diabetes. The non-obese diabetic (NOD) mouse, an animal model of type 1 diabetes, and the Nagoya-Shibata-Yasuda (NSY) mouse, a model of type 2 diabetes, are derived from the same outbred colony, Jcl:HCR, suggesting a shared susceptibility between the two types of diabetes in mice. Genetic as well as functional studies have supported the possibility that Tcf2, which encodes the transcription factor, hepatocyte nuclear factor 1beta (HNF-1beta), is a candidate gene for the common susceptibility between NSY and NOD mice. Txn, encoding thioredoxin which is a redox (reduction/oxidation)-active protein, is also a positional and functional candidate for a common susceptibility gene. To investigate whether either of these two genes is a common susceptibility gene, the coding nucleotide sequences of these two genes were compared among the NSY, NOD and control C3H strains. The coding sequence of Tcf2 of the NOD mouse was identical to that of the C3H mouse, but was different from that of the NSY mouse. The coding sequence of Txn was identical in the three strains. These data suggest that neither of the two genes is a common susceptiblity gene between type 1 and type 2 diabetes in mice.
Yamada
K
K
Department of Geriatric Medicine, Osaka University Graduate School of Medicine, Japan.
Ikegami
H
H
Kawaguchi
Y
Y
Fujisawa
T
T
Hotta
M
M
Ueda
H
H
Shintani
M
M
Nojima
K
K
Kawabata
Y
Y
Ono
M
M
Nishino
M
M
Itoi
M
M
Babaya
N
N
Shibata
M
M
Makino
S
S
Ogihara
T
T
D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary / Q000737:chemistry; D004268:DNA-Binding Proteins / Q000235:genetics*; D003922:Diabetes Mellitus, Type 1 / Q000235:genetics*; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics*; D020022:Genetic Predisposition to Disease; D006239:Haplotypes; D051539:Hepatocyte Nuclear Factor 1-beta; D051379:Mice; D008809:Mice, Inbred C3H; D008813:Mice, Inbred ICR; D016688:Mice, Inbred NOD; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA; D013879:Thioredoxins / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics*
D018076:DNA, Complementary; D004268:DNA-Binding Proteins; C496496:Hnf1b protein, mouse; D014157:Transcription Factors; D051539:Hepatocyte Nuclear Factor 1-beta; D013879:Thioredoxins