title: The preparation and crystallization of Fab fragments of a family of mouse esterolytic catalytic antibodies and their complexes with a transition-state analogue.
abstract: The Fab fragments of a family of mouse esterolytic monoclonal antibodies MS6-12, MS6-126 and MS6-164 have been obtained by digestion of whole antibodies with papain, purified and crystallized in a range of different forms either alone or in complex with a transition-state analogue. The crystals diffract X-rays to resolutions between 2.1 and 1.2 A and are suitable for structural studies. The determination of these structures could be important in understanding the different catalytic power of each of these related catalytic antibodies.
authors.affiliation: Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, Firth Court, Western Bank, Sheffield S10 2TN, England.
authors.forename: T A
authors.initials: TA
authors.lastname: Muranova
authors.forename: S N
authors.initials: SN
authors.lastname: Ruzheinikov
authors.forename: S E
authors.initials: SE
authors.lastname: Sedelnikova
authors.forename: A
authors.initials: A
authors.lastname: Moir
authors.forename: L J
authors.initials: LJ
authors.lastname: Partridge
authors.forename: H
authors.initials: H
authors.lastname: Kakinuma
authors.forename: N
authors.initials: N
authors.lastname: Takahashi
authors.forename: K
authors.initials: K
authors.lastname: Shimazaki
authors.forename: J
authors.initials: J
authors.lastname: Sun
authors.forename: Y
authors.initials: Y
authors.lastname: Nishi
authors.forename: D W
authors.initials: DW
authors.lastname: Rice
mesh_terms: D000818:Animals; D017151:Antibodies, Catalytic / Q000737:chemistry* / Q000302:isolation & purification; D000911:Antibodies, Monoclonal / Q000737:chemistry; D003460:Crystallization; D018360:Crystallography, X-Ray; D007140:Immunoglobulin Fab Fragments / Q000737:chemistry* / Q000302:isolation & purification; D051379:Mice; D011487:Protein Conformation
chemical_list: D017151:Antibodies, Catalytic; D000911:Antibodies, Monoclonal; D007140:Immunoglobulin Fab Fragments