title: Copper staining method for extracting biologically active proteins from native gels.
abstract: An attempt was made to make protein bands visible on native gel using copper staining, since such a mild staining procedure would make the entire native gel electrophoresis process non-denaturing. Copper staining not only was able to detect various proteins on native gel with reasonable sensitivity, but also made extraction and recovery of active proteins possible from the gel using a gentle procedure.
authors.affiliation: Amgen, Inc., Amgen Center, Thousand Oaks, CA 91320, USA.
authors.forename: J
authors.initials: J
authors.lastname: Wen
authors.forename: M
authors.initials: M
authors.lastname: Zhang
authors.forename: T P
authors.initials: TP
authors.lastname: Horan
authors.forename: J S
authors.initials: JS
authors.lastname: Philo
authors.forename: T
authors.initials: T
authors.lastname: Li
authors.forename: J
authors.initials: J
authors.lastname: Wypych
authors.forename: E A
authors.initials: EA
authors.lastname: Mendiaz
authors.forename: K E
authors.initials: KE
authors.lastname: Langley
authors.forename: K H
authors.initials: KH
authors.lastname: Aoki
authors.forename: M
authors.initials: M
authors.lastname: Kuwamoto
authors.forename: Y
authors.initials: Y
authors.lastname: Kita
authors.forename: T
authors.initials: T
authors.lastname: Arakawa
mesh_terms: D002850:Chromatography, Gel; D003300:Copper / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D005782:Gels / Q000737:chemistry; D011506:Proteins / Q000302:isolation & purification*; D013194:Staining and Labeling
chemical_list: D005782:Gels; D011506:Proteins; D003300:Copper