Phenotype and function of hematopoietic stem cells.
Using an in vivo selection technique, we can isolate individual cells that can repopulate the hematopoietic system of a lethally irradiated murine recipient. These cells rapidly acquire a CD34 phenotype in the animal. Progenitors in our long-term chimeras are of donor type. We also have evidence that transplantation of limiting numbers (as low as a single cell) that have this long-term repopulating ability (LTRA) can self-renew. This is demonstrated by serial transplantation of marrow from engrafted recipients 11 months post transplant into new hosts for four additional months.
Sharkis
S J
SJ
Experimental Hematopoiesis Program, Johns Hopkins Oncology Center, Baltimore, Maryland 21231, USA. ssharkis@jhmi.edu
Neutzel
S
S
Collector
M I
MI
D000818:Animals; D018952:Antigens, CD34 / Q000032:analysis; D001853:Bone Marrow / Q000473:pathology; D002455:Cell Division; D002465:Cell Movement; D005260:Female; D005456:Fluorescent Dyes; D006085:Graft Survival; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000145:classification / Q000166:cytology / Q000502:physiology*; D016130:Immunophenotyping; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D051379:Mice; D009930:Organic Chemicals; D011828:Radiation Chimera
D018952:Antigens, CD34; D005456:Fluorescent Dyes; D009930:Organic Chemicals; C070080:PKH 26