Cloning, tissue distribution and nutritional regulation of a Delta6-desaturase-like enzyme in rainbow trout.
This report describes the cloning, nutritional regulation and tissue distribution of a desaturase-like enzyme in rainbow trout (Oncorhynchus mykiss). The open reading frame of the trout desaturase-like cDNA encodes a 454-amino acid peptide that contains two membrane-spanning domains, three histidine-rich regions and a cytochrome b5 domain, which all align perfectly with the same domains located in other recently identified vertebrate Delta5- and Delta6-desaturases. Nutritional regulation of trout desaturase-like gene expression, as well as the tissue expression profile, are also similar to those observed in other vertebrate Delta5- and Delta6-desaturases. Finally, the sequence alignments between the predicted protein sequence of rainbow trout desaturase-like and other Delta6- and Delta5-desaturases revealed a high percentage identity with Delta6-desaturases (64-66% identity with vertebrate Delta6-desaturases). These results demonstrate for the first time the presence and nutritional modulation of a Delta6-desaturase-like cDNA in rainbow trout.
Seiliez
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Laboratory of Fish Nutrition, Unité mixte INRA-IFREMER, 64310, St-Pée-sur-Nivelle, France. seiliez@st-pee.inra.fr
Panserat
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Kaushik
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Bergot
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D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002462:Cell Membrane / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism; D004032:Diet; D044943:Fatty Acid Desaturases / Q000235:genetics*; D006639:Histidine / Q000378:metabolism; D050602:Linoleoyl-CoA Desaturase; D008969:Molecular Sequence Data; D017686:Oncorhynchus mykiss; D016366:Open Reading Frames; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution
D018076:DNA, Complementary; D006639:Histidine; D044943:Fatty Acid Desaturases; D050602:Linoleoyl-CoA Desaturase