title: Genotoxic, cytotoxic and developmental effects of tributyltin oxide (TBTO): an integrated approach to the evaluation of the relative sensitivities of two marine species.
abstract: Adopting an integrated approach the potential genotoxic, cytotoxic and developmental effects of tributyltin oxide (TBTO), a known endocrine-disrupting agent for neogastropods, have been evaluated in two ecologically relevant invertebrates: Mytilus edulis (blue mussel) and Platynereis dumerilli (rag worm). Following determination of the maximum tolerated dose (MTD) in terms of developmental and survival effects, the embryo-larval stages of these organisms were exposed to a range of concentrations of TBTO, and analysed for cytotoxic (proliferation rate index) and genotoxic (sister chromatid exchanges and chromosomal aberrations) effects. The study suggested that: (1) TBTO is both toxic and genotoxic to embryo-larval stages of both species; (2) at comparable concentrations, for developmental and genotoxic effects, P. dumerilii (non-target species) is more sensitive compared to M. edulis (target species); and (3) genotoxic effects are more closely tied with the development and survival of the organisms. The study emphasises the need of the evaluation of genotoxic potential of other endocrine-disrupting agents in different taxonomic groups.
authors.affiliation: Department of Biological Sciences, Plymouth Environmental Research Centre, University of Plymouth, Plymouth PL4 8AA, UK. a.jha@plymouth.ac.uk
authors.forename: A N
authors.initials: AN
authors.lastname: Jha
authors.forename: J A
authors.initials: JA
authors.lastname: Hagger
authors.forename: S J
authors.initials: SJ
authors.lastname: Hill
authors.forename: M H
authors.initials: MH
authors.lastname: Depledge
mesh_terms: D000818:Animals; D049872:Bivalvia / Q000187:drug effects* / Q000196:embryology / Q000235:genetics; D002869:Chromosome Aberrations; D011077:Polychaeta / Q000187:drug effects* / Q000196:embryology / Q000235:genetics; D012854:Sister Chromatid Exchange / Q000187:drug effects; D013045:Species Specificity; D014220:Trialkyltin Compounds / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D014220:Trialkyltin Compounds; D014874:Water Pollutants, Chemical; C005961:bis(tri-n-butyltin)oxide