title: A DNA polymorphism specific to Candida albicans strains exceptionally successful as human pathogens.
abstract: A large proportion of infection-causing isolates of the yeast Candida albicans belong to a general-purpose genotype, identifiable by fingerprinting with the moderately repetitive sequence Ca3. The high prevalence of this group -- up to 70% in some patient categories -- suggests that its members possess genetic determinants, which enhance their success as pathogens compared to other strains. To find such determinants we are comparing the genomes of representatives of the general-purpose genotype cluster with the genomes of other strains. In this paper we describe the identification of a 985 bp HpaII fragment (MU13-4) specific to general-purpose genotype strains. The fragment was present in 90% of these strains, but only in 10% of other strains. The fragment did not hybridize with probe Ca3, used to define the general-purpose cluster. It contains elevated levels of repetitive DNA. Sequences homologous to MU13-4 are dispersed throughout the chromosomes of general-purpose strains but are rarer or absent in other strains, as judged by Southern hybridization. Using the Stanford C. albicans genome database, we have placed the MU13-4 fragment next to a CARE-1 element. We also found 79 significant homologies between parts of MU13-4 and 19 other contigs. Attempts to amplify the region surrounding the polymorphic fragment in non-general-purpose genotype strains suggest, as do the hybridization data, that the polymorphism is created by a deletion in non-cluster strains. These results show that it is possible to identify polymorphisms specific to general-purpose genotype strains. Primers against the fragment will allow PCR-based discrimination between general-purpose genotype strains and other strains, facilitating investigations aimed at determining morbidity and mortality caused by general-purpose genotype strains compared to other strains.
authors.affiliation: Institute of Molecular BioSciences, College of Sciences, Massey University, Palmerston North, New Zealand.
authors.forename: L
authors.initials: L
authors.lastname: Giblin
authors.forename: A
authors.initials: A
authors.lastname: Edelmann
authors.forename: N
authors.initials: N
authors.lastname: Zhang
authors.forename: N B
authors.initials: NB
authors.lastname: von Maltzahn
authors.forename: S B
authors.initials: SB
authors.lastname: Cleland
authors.forename: P A
authors.initials: PA
authors.lastname: Sullivan
authors.forename: J
authors.initials: J
authors.lastname: Schmid
mesh_terms: D001483:Base Sequence; D002176:Candida albicans / Q000235:genetics* / Q000472:pathogenicity; D015825:Chromosomes, Fungal / Q000235:genetics; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics*; D018628:Gene Dosage; D005838:Genotype; D008969:Molecular Sequence Data; D010802:Phylogeny; D011110:Polymorphism, Genetic; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
chemical_list: D004271:DNA, Fungal