Leukaemia inhibitory factor and interleukin 6 inhibit secretion of prolactin and growth hormone by rat pituitary MtT/SM cells.
The rat pituitary cell line, MtT/SM, has the characteristics of somatomammotrophs. The cells secrete both prolactin (PRL) and growth hormone (GH). We examined the effects of cytokines such as leukaemia inhibitory factor (LIF), interleukin 6 (IL-6), oncostatin M and interleukin 11 on the secretion of these hormones by the cells. These cytokines stimulate proliferation of the cells and inhibit the secretion of PRL by 70-80% and that of GH by 50%. They induce tyrosine phosphorylation of STAT3 in the cells. The cells containing PRL or GH decreased at 48 h after treatment of the cells with LIF or IL-6. These results suggest that the LIF/IL-6 family of cytokines inhibits the functions of mammotrophs and somatotrophs in the pituitary gland.
Tomida
M
M
Laboratory of Carcinogenesis and Cancer Prevention, Saitama Cancer Center Research Institute, Ina, Saitama, 362-0806, Japan. tomida@cancer-c.pref.saitama.jp
Yoshida
U
U
Mogi
C
C
Maruyama
M
M
Goda
H
H
Hatta
Y
Y
Inoue
K
K
D000818:Animals; D002455:Cell Division / Q000276:immunology; D004268:DNA-Binding Proteins / Q000378:metabolism; D013006:Growth Hormone / Q000037:antagonists & inhibitors* / Q000096:biosynthesis / Q000378:metabolism*; D006131:Growth Inhibitors / Q000494:pharmacology*; D006801:Humans; D007150:Immunohistochemistry; D017370:Interleukin-11 / Q000494:pharmacology; D015850:Interleukin-6 / Q000494:pharmacology*; D053605:Leukemia Inhibitory Factor; D008222:Lymphokines / Q000494:pharmacology*; D053683:Oncostatin M; D010455:Peptides / Q000494:pharmacology; D010766:Phosphorylation; D010911:Pituitary Neoplasms / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D011388:Prolactin / Q000037:antagonists & inhibitors* / Q000096:biosynthesis / Q000378:metabolism*; D051381:Rats; D011916:Rats, Inbred F344; D050796:STAT3 Transcription Factor; D015534:Trans-Activators / Q000378:metabolism; D014407:Tumor Cells, Cultured
D004268:DNA-Binding Proteins; D006131:Growth Inhibitors; D017370:Interleukin-11; D015850:Interleukin-6; C507837:LIF protein, human; D053605:Leukemia Inhibitory Factor; D008222:Lymphokines; C508351:OSM protein, human; D010455:Peptides; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; C494088:Stat3 protein, rat; D015534:Trans-Activators; D053683:Oncostatin M; D011388:Prolactin; D013006:Growth Hormone