Effects of a highly toxic coplanar polychlorinated biphenyl, 3,3',4,4',5-pentachlorobiphenyl on intermediary metabolism: reduced triose phosphate content in rat liver cytosol.
The effects of a highly toxic coplanar polychlorinated biphenyl, 3,3',4,4',5-pentachlorobiphenyl (PenCB), on triose phosphate metabolizing enzymes were studied. Male Wistar rats received 25 mg/kg PenCB, i.p. At this dose the compound provokes a wasting syndrome. The activity of triose phosphate metabolizing enzymes, glyceraldehyde-3-phosphate dehydrogenase, triose phosphate isomerase, glycerokinase, transaldolase and transketolase were significantly reduced by PenCB treatment to 50%, 60%, 50%, 70% and 40% of free-fed controls, respectively. An inhibition study with pyrazol, a specific inhibitor of alcohol dehydrogenase (ADH), showed that ADH makes about a 30% contribution to the formation of glycerol-3-phosphate from glyceraldehyde-3-phosphate. Our current study revealed that PenCB suppresses ADH at the protein expression level. The reduced formation of glycerol-3-phosphate from glyceraldehyde dehydrogenase by PenCB could be due to the suppression of ADH. The triose phosphate content in the liver cytosol of PenCB-treated rats was significantly lower than in free-fed controls. The suppression of triose phosphate metabolism could be a cause of the wasting syndrome provoked by highly toxic coplanar PCB.
Ishii
Y
Y
Graduate School of Pharmaceutical Sciences, Kyushu University, Higashi-ku, Fukuoka, 812-8582, Japan.
Kato
H
H
Hatsumura
M
M
Ishida
T
T
Ariyoshi
N
N
Yamada
H
H
Oguri
K
K
D000818:Animals; D003600:Cytosol / Q000378:metabolism; D005991:Glycerol Kinase / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D008297:Male; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism; D011078:Polychlorinated Biphenyls / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013403:Sugar Phosphates / Q000378:metabolism*; D014153:Transaldolase / Q000378:metabolism; D014174:Transketolase / Q000378:metabolism; D014305:Triose-Phosphate Isomerase / Q000378:metabolism
D013403:Sugar Phosphates; D011078:Polychlorinated Biphenyls; D014174:Transketolase; D014153:Transaldolase; D005991:Glycerol Kinase; C055686:glyceraldehyde 3-phosphate phosphatase; D010744:Phosphoric Monoester Hydrolases; D014305:Triose-Phosphate Isomerase; C023035:3,4,5,3',4'-pentachlorobiphenyl