Nine novel conserved motifs in BRCA1 identified by the chicken orthologue.
The breast cancer susceptibility gene BRCA1 encodes a multifunctional protein that is mutated in many hereditary breast and ovarian cancers. We have cloned a homologue of the human BRCA1 gene from chicken; the gene encodes a 1749 amino acid protein that is 33% identical to human BRCA1. Phylogenetic analyses of the chicken and mammalian proteins indicate that the chicken gene is a bona fide BRCA1 orthologue, the first to be described from a non-mammal. Most of the chicken protein has diverged considerably from its mammalian orthologues, although the RING and BRCT repeat regions are highly conserved. This marked overall sequence divergence has allowed us to identify nine additional highly-conserved motifs (ranging from 8--56 amino acids in length) which are likely important for BRCA1 function.
Orelli
B J
BJ
Department of Radiation and Cellular Oncology, University of Chicago, 920 E 58th Street, Chicago, Illinois, IL 60637, USA.
Logsdon
J M
JM
Bishop
D K
DK
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D030161:Avian Proteins; D019313:BRCA1 Protein / Q000737:chemistry* / Q000235:genetics*; D002642:Chick Embryo; D002645:Chickens / Q000235:genetics*; D003001:Cloning, Molecular; D017124:Conserved Sequence; D019398:Genes, BRCA1; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid
D030161:Avian Proteins; D019313:BRCA1 Protein; C432045:BRCA1 protein, chicken