Characterization of five tomato phospholipase D cDNAs: rapid and specific expression of LePLDbeta1 on elicitation with xylanase.
Phospholipase D (PLD, EC 3.1.4.4.) has been implicated in a variety of plant processes, including signalling. In Arabidopsis thaliana a PLD gene family has been described and individual members classified into alpha-, beta- and gamma-classes. Here we describe a second PLD gene family in tomato (Lycopersicon esculentum) that includes three alpha- and two beta-classes. Different expression patterns in plant organs were observed for each PLD. In testing a variety of stress treatments on tomato cell suspensions, PLDbeta1 mRNA was found to rapidly and specifically accumulate in response to the fungal elicitor xylanase. The greatest increase was found 2 h after treatment with 100 microg m1(-1) xylanase (ninefold). In vivo PLD activity increased nearly threefold over a 1.5 h period of treatment. When the elicitor was injected into tomato leaves, PLDbeta1 mRNA accumulation peaked at 2 h (threefold increase), before decreasing to background levels within 72 h. Mutant, non-active xylanase was as effective as the active enzyme in eliciting a response, suggesting that xylanase itself, and not the products resulting from its activity, functioned as an elicitor. When chitotetraose was used as elicitor, no PLDbeta1 mRNA accumulation was observed, thus it is not a general response to elicitation. Together these data show that PLD genes are differentially regulated, reflecting potential differences in cellular function. The possibility that PLDbeta1 is a signalling enzyme is discussed.
Laxalt
A M
AM
Department of Plant Physiology, Swammerdam Institute for Life Sciences, University of Amsterdam, The Netherlands. laxalt@science.uva.nl
ter Riet
B
B
Verdonk
J C
JC
Parigi
L
L
Tameling
W I
WI
Vossen
J
J
Haring
M
M
Musgrave
A
A
Munnik
T
T
D000595:Amino Acid Sequence; D002478:Cells, Cultured; D003001:Cloning, Molecular; D003080:Cold Temperature; D018076:DNA, Complementary; D018744:DNA, Plant; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D018551:Solanum lycopersicum / Q000201:enzymology* / Q000235:genetics; D008969:Molecular Sequence Data; D005810:Multigene Family; D009997:Osmotic Pressure; D010739:Phospholipase D / Q000235:genetics*; D018515:Plant Leaves / Q000187:drug effects / Q000201:enzymology; D012333:RNA, Messenger / Q000378:metabolism; D016415:Sequence Alignment; D043325:Xylan Endo-1,3-beta-Xylosidase; D014995:Xylosidases / Q000378:metabolism
D018076:DNA, Complementary; D018744:DNA, Plant; D012333:RNA, Messenger; D010739:Phospholipase D; D014995:Xylosidases; D043325:Xylan Endo-1,3-beta-Xylosidase