title: A very efficient method to cleave Lipid A and saccharide components in bacterial lipopolysaccharides.
abstract: A novel mild procedure for the selective cleavage of ketosidic linkages is developed using ceric ammonium nitrate (CAN) in anhydrous N,N-dimethylformamide. Its application to lipopolysaccharides (LPS) is very significant because in the so far investigated LPS, the connection between the Lipid A region and the oligo(poly)saccharide part is always a keto-sugar. This procedure has been tested on LPS of Escherichia coli which contains Kdo as a linker between Lipid A and OPS and on Acinetobacter haemoliticus which contains D-glycero-D-talo-2-octulopyranosonic acid (Ko) as a linker and it performed efficiently in both cases.
authors.affiliation: Dipartimento di Chimica Organica e Biochimica, Università di Napoli 'Federico II', via Cintia, I-80126, Naples, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Manzo
authors.forename: A
authors.initials: A
authors.lastname: Molinaro
authors.forename: E
authors.initials: E
authors.lastname: Bedini
authors.forename: C
authors.initials: C
authors.lastname: De Castro
authors.forename: M
authors.initials: M
authors.lastname: Parrilli
mesh_terms: D000150:Acinetobacter / Q000737:chemistry; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000737:chemistry*; D002563:Cerium; D004126:Dimethylformamide; D004926:Escherichia coli / Q000737:chemistry; D008050:Lipid A / Q000737:chemistry*; D008070:Lipopolysaccharides / Q000737:chemistry*; D008969:Molecular Sequence Data
chemical_list: D002241:Carbohydrates; D008050:Lipid A; D008070:Lipopolysaccharides; D002563:Cerium; D004126:Dimethylformamide; C004653:ceric ammonium nitrate