Comparative genomic hybridization in polycythemia vera and essential thrombocytosis patients.
Polycythemia vera (PV) and essential thrombocytosis (ET) are clonal chronic myeloproliferative disorders originating from a multipotent stem cell. Bone marrow examinations reveal chromosomal abnormalities in 15-43% of PV patients and 5% of ET patients, but no specific recurring abnormality has been found to date. We aimed to find cytogenetic aberrations in PV and ET by comparative genomic hybridization (CGH), a relatively new molecular cytogenetic technique. In this study, CGH analysis was performed on peripheral blood leukocytes of 12 PV patients and 8 ET patients. One patient (8.3%) with PV had an abnormal karyotype with a deletion in 7q11.2 and one patient with ET (12.5%) had a gain in 18p. Peripheral blood analysis by CGH revealed a low frequency of cytogenetic abnormalities in PV and ET patients. However, using CGH we were able to detect two cytogenetic aberrations that were not reported previously in these disorders.
Herishanu
Y
Y
Department of Medicine, Meir Hospital Kfar-Saba and Sackler School of Medicine, Tel-Aviv University, Tel-Aviv, Israel.
Lishner
M
M
Bomstein
Y
Y
Kitay-Cohen
Y
Y
Fejgin
M D
MD
Gaber
E
E
Amiel
A
A
D000368:Aged; D000369:Aged, 80 and over; D002869:Chromosome Aberrations; D005260:Female; D006801:Humans; D006918:Hydroxyurea / Q000627:therapeutic use; D008297:Male; D008875:Middle Aged; D009693:Nucleic Acid Hybridization; D018962:Phlebotomy; D010761:Phosphorus Radioisotopes / Q000627:therapeutic use; D011087:Polycythemia Vera / Q000097:blood / Q000235:genetics* / Q000628:therapy; D013922:Thrombocytosis / Q000097:blood / Q000235:genetics* / Q000628:therapy
D010761:Phosphorus Radioisotopes; D006918:Hydroxyurea