Cyclin A and Ki-67 with DNA content in benign and malignant prostatic epithelial lesions.
OBJECTIVE
To evaluate the usefulness of immunohistochemical staining of cyclin A and Ki-67 together with DNA content in the classification of benign prostatic hyperplasia, prostatic intraepithelial neoplasm (PIN) and prostatic carcinoma foci and to compare these parameters with each other and with parameters obtained from conventional histopathology.


METHODS
We selected 37 carcinoma, 18 PIN and 8 hyperplastic foci from prostatectomies done during 1996 and 1997 at Turku University Central Hospital. Cyclin A and Ki-67 staining was assessed by immunohistochemistry and DNA content by image cytometry.


RESULTS
The hyperplastic, PIN and carcinoma foci differed clearly in their 2.5c exceeding rates, image cytometric proliferation indices and staining indices for cyclin A and Ki-67. No significant differences were found between these histologic entities in their modal DNA ploidy values. In carcinomas, cyclin A and Ki-67 indices differed between low, intermediate and high Gleason and World Health Organization grading groups. Diploid and tetraploid carcinomas had similar cyclin A and Ki-67 indices, which differed from those of aneuploid carcinomas.


CONCLUSIONS
The 2.5c exceeding rate and image cytometric proliferation index as well as the cyclin A and Ki-67 indices differed significantly between different types of prostatic lesions. Cyclin A and Ki-67 had good correlations with the histologic grade of carcinoma.
Mirtti
T
T
Department of Pathology, University of Turku, Turku, Finland.
Kallajoki
M
M
Aaltonen
M
M
Alanen
K
K
D014408:Biomarkers, Tumor; D019925:Cyclin A / Q000032:analysis*; D004273:DNA, Neoplasm / Q000032:analysis; D003937:Diagnosis, Differential; D006801:Humans; D019044:Image Cytometry; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000032:analysis*; D008297:Male; D011003:Ploidies; D011469:Prostatic Diseases / Q000235:genetics / Q000473:pathology*; D011470:Prostatic Hyperplasia / Q000235:genetics / Q000473:pathology; D019048:Prostatic Intraepithelial Neoplasia / Q000737:chemistry / Q000235:genetics / Q000473:pathology; D011471:Prostatic Neoplasms / Q000737:chemistry / Q000235:genetics / Q000473:pathology
D014408:Biomarkers, Tumor; D019925:Cyclin A; D004273:DNA, Neoplasm; D019394:Ki-67 Antigen