FMS*Calciumfluor increases alkaline phosphatase expression during osteogenesis in vitro of tibia-derived rat osteoblasts by activation of G alpha 0/G alpha i proteins.
We studied the involvement of G-proteins in transducing the inductive signal generated by treatment of tibial-derived neonatal rat osteoblasts (ROB) cultured in vitro with FMS*Calciumfluor, a homeopathic preparation utilized in the therapy of osteoporosis. We previously reported that FMS*Calciumfluor acts as inducer and potentiator of osteogenic differentiation in vitro, among other effects, by increasing the expression of Alkaline phosphatase (AP). We utilized Pertussis Toxin (PTX), an inhibitor of G alpha 0/G alpha i proteins, Mastoparan 7, an activator of G alpha 0/G alpha i proteins and Cholera Toxin (CTX), a stimulator of G alpha s protein to show involvement of specific G proteins in the inductive effect on AP of FMS*Calciumfluor. We here show that the increase in AP expression induced by FMS*Calciumfluor is dependent on the activation of G alpha 0/G alpha i proteins, while it is unaffected by the activation stage of the G alpha s protein. Moreover, we show that the expression of endogenous AP during osteogenesis in vitro is regulated independently from G proteins, and unaffected by their activation stage and therefore that treatment with FMS*Calciumfluor activates a new pathway of cellular response.
Palermo
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C
Dipartimento di Oncologia, Biologia e Genetica, Università di Genova.
Manduca
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P
D000469:Alkaline Phosphatase / Q000096:biosynthesis*; D000818:Animals; D002118:Calcium / Q000502:physiology*; D002478:Cells, Cultured; D020962:Heterotrimeric GTP-Binding Proteins / Q000502:physiology*; D010006:Osteoblasts / Q000502:physiology*; D010012:Osteogenesis / Q000187:drug effects*; D037342:Pertussis Toxin; D051381:Rats; D013977:Tibia / Q000502:physiology; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
D010566:Virulence Factors, Bordetella; D037342:Pertussis Toxin; D000469:Alkaline Phosphatase; D020962:Heterotrimeric GTP-Binding Proteins; D002118:Calcium