Crystallization and preliminary X-ray study of endopolygalacturonase from the pathogenic fungus Stereum purpureum.
Crystals of endopolygalacturonase I from Stereum purpureum have been obtained by the vapour-diffusion method. Prior to crystallization work, endopolygalacturonase I was deglycosylated with endo-beta-N-acetylglucosaminidase H. The crystal diffracts to ultrahigh (0.96 A) resolution using synchrotron radiation and belongs to space group P1, with unit-cell parameters a = 37.26, b = 46.34, c = 52.05 A, alpha = 67.17, beta = 72.44, gamma = 68.90 degrees.
Shimizu
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Kinetic Crystallography Research Team, RIKEN, Harima Institute at SPring-8, 1-1-1 Kouto, Mikazuki-cho, Sayo-gun, Hyogo 679-5148, Japan.
Nakatsu
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Miyairi
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K
Okuno
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Kato
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H
D001487:Basidiomycota / Q000201:enzymology*; D003460:Crystallization; D018360:Crystallography, X-Ray; D011096:Polygalacturonase / Q000737:chemistry*; D011487:Protein Conformation
D011096:Polygalacturonase