Cloning and biochemical characterization of a class A beta-lactamase from Prevotella intermedia.
The gene encoding a beta-lactamase of Prevotella intermedia was cloned and sequenced. This gene, called cfxA2, shared 98% identity with cfxA, the structural gene of a beta-lactamase previously described in Bacteroides vulgatus. The deduced protein sequence had a K272E substitution. CfxA2 had the characteristics of class A, group 2e beta-lactamases.
Madinier
I
I
Laboratoire de Pathobiologie Orale, Faculté de Chirurgie Dentaire, 06357 Nice Cedex 4, France.
Fosse
T
T
Giudicelli
J
J
Labia
R
R
D000595:Amino Acid Sequence; D001439:Bacteroides / Q000201:enzymology; D003001:Cloning, Molecular; D017931:DNA Primers / Q000737:chemistry; D004269:DNA, Bacterial / Q000032:analysis; D006801:Humans; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D018713:Prevotella intermedia / Q000201:enzymology*; D017386:Sequence Homology, Amino Acid; D001618:beta-Lactamases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism
D017931:DNA Primers; D004269:DNA, Bacterial; D001618:beta-Lactamases