title: Random amplified polymorphic DNA analysis of Acinetobacter species isolated from worn contact lenses.
abstract: The purpose of this study was to explore the genotypes of Acinetobacter species and to compare the genotypes and phenotypes of the Acinetobacter isolated from contact lens wearers. Nineteen strains were used in the study, 13 were isolated from patients experiencing an adverse response event, and six strains were isolated from asymptomatic contact lens wearers. Random amplified polymorphic DNA and phenotypic analyses were carried out using commercially available kits. Random amplified polymorphic DNA analysis showed a higher discrimination power compared with the phenotypic analysis. The test strains were loosely clustered into six genotypic groups with no definite relation to any clinical events. These results indicate that many genotypes of Acinetobacter can cause adverse responses, and the initial source of the microorganisms rather than their clinical sequelae may determine classification.
authors.affiliation: Cooperative Research Centre for Eye Research and Technology, The University of New South Wales, Sydney, Australia. k.corrigan@cclru.unsw.edu.au
authors.forename: K M
authors.initials: KM
authors.lastname: Corrigan
authors.forename: H
authors.initials: H
authors.lastname: Zhu
authors.forename: M D
authors.initials: MD
authors.lastname: Willcox
mesh_terms: D000150:Acinetobacter / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D003261:Contact Lenses / Q000382:microbiology*; D004269:DNA, Bacterial / Q000032:analysis*; D005838:Genotype; D006801:Humans; D010641:Phenotype; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods
chemical_list: D004269:DNA, Bacterial