Isolation and immunochemical studies of dog trypsin.
Dog trypsin (EC 3.4.4.4) was isolated from dog pancreatic juice on SP-Sephadex C-50. The preparation was homogeneous on disc electrophoresis at pH 4.3. On agarose gel electrophoresis at pH 8.6, dog pancreas trypsinogen had the mobility of an alpha 2-globulin and trypsin the mobility of a beta-globulin. On gel filtration on Sephadex G-75 at pH 4.0, dog trypsin was eluted in the same fractions as bovine trypsin. It was inhibited by soybean trypsin inhibitor. Rabbit anti-dog trypsin inhibited the caseinolytic activity of bovine trypsin by about 60%.
Ohlsson
K
K
Department of Clinical Chemistry, University of Lund, Malmö General Hospital, Malmö, Sweden.
D000818:Animals; D000906:Antibodies; D002417:Cattle; D002850:Chromatography, Gel; D004285:Dogs; D004587:Electrophoresis, Agar Gel; D004589:Electrophoresis, Disc; D010189:Pancreatic Juice / Q000201:enzymology*; D011817:Rabbits; D014357:Trypsin / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism
D000906:Antibodies; D014357:Trypsin