title: A technique of heterotopic, infrarenal heart transplantation with double anastomosis in mice.
abstract: Mouse heart transplantation is frequently used to investigate mechanisms of rejection and immune response. A fully vascularized technique is presented in which the graft ascending aorta is anastomosed end-to-side to the recipient's aorta and the pulmonary aorta is anastomosed end-to-side to the inferior vena cava using 11-0 Ethilon suture. We suggest that this double anastomosis technique is a valuable tool to investigate immune mechanisms in mice.
authors.affiliation: Department of Cardiovascular Surgery, University of Freiburg, Freiburg, Germany. doenst@ch11.ukl.uni-freiburg.de
authors.forename: T
authors.initials: T
authors.lastname: Doenst
authors.forename: C
authors.initials: C
authors.lastname: Schlensak
authors.forename: J L
authors.initials: JL
authors.lastname: Kobba
authors.forename: F
authors.initials: F
authors.lastname: Beyersdorf
mesh_terms: D000714:Anastomosis, Surgical / Q000379:methods*; D000818:Animals; D001012:Aorta, Abdominal / Q000601:surgery; D006085:Graft Survival; D006324:Heart Arrest, Induced / Q000009:adverse effects; D016027:Heart Transplantation / Q000009:adverse effects / Q000379:methods*; D051379:Mice; D008807:Mice, Inbred BALB C; D023421:Models, Animal; D017202:Myocardial Ischemia / Q000209:etiology / Q000473:pathology; D009206:Myocardium / Q000473:pathology; D016042:Transplantation, Heterotopic / Q000379:methods*; D014682:Vena Cava, Inferior / Q000601:surgery*