Construction of an efficient amylolytic industrial yeast strain containing DNA exclusively derived from yeast.
An amylolytic industrial yeast strain of Saccharomyces cerevisiae containing the Schwanniomyces occidentalis SWA2 amylase gene was generated. The new strain contains DNA derived exclusively from yeast and expresses a high starch hydrolyzing activity. Yeast transformation was carried out by an integrative process targeted to a dispensable upstream region of the ILV2 locus, which determines sulfometuron resistance. The SWA2 enzyme was constitutively expressed under the ADH1 promoter. The growth, substrate utilization and fermentative capacity of this organism are described.
Marín
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Centro de Biología Molecular 'Severo Ochoa', Departamento de Biología Molecular (CSIC-UAM), Universidad Autónoma Madrid, Cantoblanco, 28049, Madrid, Spain.
Jiménez
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Fernández Lobato
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M
D001709:Biotechnology / Q000379:methods; D004271:DNA, Fungal / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D005285:Fermentation; D005524:Food Technology; D005800:Genes, Fungal / Q000235:genetics*; D007218:Industrial Microbiology / Q000379:methods; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D004718:Saccharomycetales / Q000235:genetics*; D013213:Starch / Q000378:metabolism; D013997:Time Factors; D014170:Transformation, Genetic; D000516:alpha-Amylases / Q000235:genetics / Q000378:metabolism*
D004271:DNA, Fungal; D013213:Starch; D000516:alpha-Amylases