Lipolytic activity of porcine pancreas lipase on fatty acid esters of dialkylglycerols: a structural basis for the design of new substrates for the assay of pancreatic lipases activity.
For the design of new synthetic substrates for the assay of pancreatic lipases activity, acyl dialkylglycerols of variable chain length were prepared. Titrimetric assay of these substrates showed the highest lipolytic activity of porcine pancreas lipase (pPL) with butanoyl dibutylglycerol. The activity is lower but comparable to that shown by pPL towards the classical substrate tributyrin. The 4-nitrophenylcarbonate of 1,2-di-O-butylglycerol, has been prepared and proposed as synthetic substrate for a new spectrophotometric assay of pancreatic lipases.
Ciuffreda
P
P
Dipartimento di Scenze Precliniche LITA Vialba-Via G.B. Grassi, 74-20157, Milan, Italy.
Loseto
A
A
Manzocchi
A
A
Santaniello
E
E
D000818:Animals; D015195:Drug Design; D005227:Fatty Acids / Q000138:chemical synthesis / Q000737:chemistry; D005989:Glycerides / Q000138:chemical synthesis / Q000737:chemistry; D006868:Hydrolysis; D066298:In Vitro Techniques; D008049:Lipase / Q000032:analysis / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D010179:Pancreas / Q000201:enzymology*; D013053:Spectrophotometry; D013379:Substrate Specificity; D013552:Swine
D005227:Fatty Acids; D005989:Glycerides; D008049:Lipase