Changes in some blood micronutrients, leukocytes and neutrophil expression of adhesion molecules in periparturient dairy cows.
Dairy cows are highly susceptible to infectious diseases, like mastitis, during the period around calving. Although factors contributing to increased susceptibility to infection have not been fully elucidated, impaired neutrophil recruitment to the site of infection and changes in the concentrations of some micronutrients related with the function of the immune defence has been implicated. Most of the current information is based on studies outside the Nordic countries where the conditions for dairy cows are different. Therefore, the aim of the study was to evaluate changes in blood concentrations of the vitamins A and E, the minerals calcium (Ca), phosphorous (P), and magnesium (Mg), the electrolytes potassium (K) and sodium (Na) and the trace elements selenium (Se), copper (Cu) and zinc (Zn), as well as changes in total and differential white blood cell counts (WBC) and expression of the adhesion molecules CD62L and CD18 on blood neutrophils in Swedish dairy cows during the period around calving. Blood samples were taken from 10 cows one month before expected calving, at calving and one month after calving. The results were mainly in line with reports from other countries. The concentrations of vitamins A and E, and of Zn, Ca and P decreased significantly at calving, while Se, Cu, and Na increased. Leukocytosis was detected at calving, mainly explained by neutrophilia, but also by monocytosis. The numbers of lymphocytes tended to decrease at the same time. The mean fluorescent intensity (MFI) of CD62L and CD18 molecules on blood neutrophils remained constant over time. The proportion of CD62L+ neutrophils decreased significantly at calving. The animals were fed according to, or above, their requirements. Therefore, changes in blood levels of vitamins, minerals and trace elements were mainly in response to colostrum formation, changes in dry matter intake, and ruminal metabolism around calving. Decreased levels of vitamins A and E, and of Zn at calving might have negative implications for the functions of the immune defence. The lower proportion of CD62L+ neutrophils at calving may result in less migration of blood neutrophils into the tissues, and might contribute to the increased susceptibility to infections at this time.
Meglia
G E
GE
Department of Obstetrics and Gynaecology, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, Sweden.
Johannisson
A
A
Petersson
L
L
Waller
K P
KP
D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D018821:CD18 Antigens / Q000097:blood; D002417:Cattle / Q000097:blood* / Q000502:physiology; D004198:Disease Susceptibility / Q000662:veterinary*; D005260:Female; D019041:L-Selectin / Q000097:blood; D007774:Lactation; D007958:Leukocyte Count / Q000662:veterinary; D007962:Leukocytes / Q000378:metabolism*; D008903:Minerals / Q000097:blood; D009504:Neutrophils / Q000378:metabolism*; D011247:Pregnancy; D012016:Reference Values; D014131:Trace Elements / Q000097:blood; D014815:Vitamins / Q000097:blood
D018821:CD18 Antigens; D008903:Minerals; D014131:Trace Elements; D014815:Vitamins; D019041:L-Selectin