Genomics of the human genes encoding four TAFII subunits of TFIID, the three subunits of TFIIA, as well as CDK8 and SURB7.
By in situ chromosomal hybridization, and by somatic cell and radiation hybrid analysis, we have determined the genomic position of the human genes encoding four TAFII subunits of TFIID (TAFII150, TAFII105, TAFII68, TAFII18), the three subunits of TFIIA (TFIIA35 and TFIIA19, both encoded by the same gene, and TFIIA12), CDK8, and SURB7. All of these proteins are bona fide components of human class II holoenzymes as well as targets of signal transduction pathways that regulate genome expression. The genes encoding them are present in the human genome in a single copy and are localized at 8q23, 18q11.2, 17q11.1-11.2, 1p21, 14q31, 15q21-23, 13q12, and 12p12, respectively. We have mapped all of them to chromosomal regions where hereditary genetic diseases have been localized or which are involved in malignancies, which makes them potential candidates for a causal involvement in these phenotypes.
Di Pietro
C
C
Dipartimento di Scienze Biomediche, Sezione di Biologia generale, cellulare e di Genetica molecolare, Università di Catania, 95124 Catania, Italy.
Rapisarda
A
A
Bonaiuto
C
C
Lizzio
M N
MN
Engel
H
H
Amico
V
V
Scalia
M
M
Amato
A
A
Grzeschik
K H
KH
Sichel
G
G
Purrello
M
M
D000015:Abnormalities, Multiple / Q000235:genetics; D002877:Chromosomes, Human; D056850:Cyclin-Dependent Kinase 8; D018844:Cyclin-Dependent Kinases; D004268:DNA-Binding Proteins / Q000235:genetics*; D018628:Gene Dosage; D015894:Genome, Human; D006801:Humans; D017403:In Situ Hybridization; D056892:Mediator Complex; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D021101:Radiation Hybrid Mapping; D035561:Transcription Factor TFIIA; D035362:Transcription Factor TFIID; D014157:Transcription Factors / Q000235:genetics*; D019473:Transcription Factors, TFII / Q000235:genetics*; D014158:Transcription, Genetic / Q000235:genetics*
D004268:DNA-Binding Proteins; C098617:MED21 protein, human; D056892:Mediator Complex; D035561:Transcription Factor TFIIA; D035362:Transcription Factor TFIID; D014157:Transcription Factors; D019473:Transcription Factors, TFII; D017346:Protein Serine-Threonine Kinases; C095686:CDK8 protein, human; D056850:Cyclin-Dependent Kinase 8; D018844:Cyclin-Dependent Kinases