title: A multi-modality assay platform for ultra-high throughput screening.
abstract: The demand for increased throughput during primary screening using less reagents is changing the way of drug discovery. Searching for hits using high throughput screening in 96-well format plates is being replaced by the use of higher density plates, such as 384 and 1536-well formats. The analysis of radiometric assays by scintillation counters is becoming limiting since only 12 wells can be counted at a time. Charged coupled device (CCD) camera based instruments, that image the whole plate in one exposure, speed up detection and are compatible with any microplate footprint. Researchers are also demanding a choice of detection methods, including fluorescence, luminescence and radioactivity, and require imagers suitable for all applications. LEADseeker Homogenous Imaging System is a multi-modality platform offering imaging technology and assay toolboxes for radiometric, fluorescent and luminescent based assays. LEADseeker allows the very rapid analysis of high density formats enabling ultra-high throughput screening of a range of biological assays. Research areas that can be studied using this system include enzyme assays, receptor binding and molecular interactions.
authors.affiliation: Amersham Pharmacia Biotech UK Limited, Little Chalfont, Buckinghamshire, England. leadseeker@eu.apbiotech.com
authors.forename: A
authors.initials: A
authors.lastname: Fowler
authors.forename: I
authors.initials: I
authors.lastname: Davies
authors.forename: C
authors.initials: C
authors.lastname: Norey
mesh_terms: D015195:Drug Design; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D007091:Image Processing, Computer-Assisted; D007202:Indicators and Reagents; D010600:Pharmacology / Q000379:methods*; D012815:Signal Processing, Computer-Assisted
chemical_list: D007202:Indicators and Reagents