Arabidopsis thaliana ecotype Cvi shows an increased tolerance to photo-oxidative stress and contains a new chloroplastic copper/zinc superoxide dismutase isoenzyme.
A new chloroplastic Cu/Zn-superoxide dismutase (SOD) isoenzyme was identified in Arabidopsis thaliana ecotype Cvi. Genetic analyses indicated that the new isoenzyme was encoded by a Cvi-specific allele of Csd2 that was named Csd2-2. Paraquat treatments of A. thaliana ecotypes Ler and Cvi resulted in higher levels of chloroplastic Cu/Zn-SOD activity in Cvi, suggesting that the Cvi isoenzyme has a higher stability and/or turnover rate than the Ler variant under photo-oxidative conditions. In addition, Cvi showed a higher tolerance to paraquat treatments. Hybrid plant populations expressing Csd2-2 also exhibited an increased tolerance, suggesting that the Cvi isoenzyme is one of the factors that contribute to a better fitness in photo-oxidative stress conditions.
Abarca
D
D
Departamento de Biología Vegetal, Facultad de Biología, Universidad de Alcalá, 28871-Madrid, Spain. mdolores.abarca@uah.es
Roldán
M
M
Martín
M
M
Sabater
B
B
D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics / Q000502:physiology*; D015152:Blotting, Northern; D002736:Chloroplasts / Q000201:enzymology*; D018744:DNA, Plant / Q000032:analysis; D004351:Drug Resistance; D006540:Herbicides / Q000494:pharmacology; D007527:Isoenzymes / Q000187:drug effects / Q000378:metabolism; D008027:Light; D018384:Oxidative Stress; D010269:Paraquat / Q000494:pharmacology; D018515:Plant Leaves / Q000187:drug effects; D018749:RNA, Plant / Q000032:analysis; D013482:Superoxide Dismutase / Q000378:metabolism*
D018744:DNA, Plant; D006540:Herbicides; D007527:Isoenzymes; D018749:RNA, Plant; D013482:Superoxide Dismutase; D010269:Paraquat