Huperzine A protects rat pheochromocytoma cells against oxygen-glucose deprivation.
The effect of huperzine A (HupA) on oxygen-glucose deprivation (OGD)-induced injury was investigated in the rat pheochromocytoma cell line PC12. OGD for 3 h and reoxygenation for 24 h triggered apoptosis characterized by chromatin condensation, nucleus fragmentation and DNA laddering. The temporal profile of c-jun, p53, bcl-2 and bax mRNA after OGD indicated that these genes played important roles in apoptosis. Pre-incubation of the cells for 2 h with 1 microM HupA significantly attenuated apoptosis. The same treatment also reduced the up-regulation of c-jun and bax as well as the down-regulation of bcl-2. These data suggest the ability of HupA to attenuate apoptosis induced by OGD may result from its capability to alter the expression of apoptosis-related genes.
Zhou
J
J
State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 294 Tai-yuan Road, Shanghai 200031, PR China.
Fu
Y
Y
Tang
X C
XC
D000470:Alkaloids; D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology; D015687:Cell Hypoxia / Q000187:drug effects / Q000502:physiology; D053938:DNA Fragmentation / Q000187:drug effects / Q000502:physiology; D015870:Gene Expression / Q000187:drug effects / Q000502:physiology; D019254:Genes, bcl-2 / Q000187:drug effects / Q000502:physiology; D016758:Genes, jun / Q000187:drug effects / Q000502:physiology; D016158:Genes, p53 / Q000187:drug effects / Q000502:physiology; D018696:Neuroprotective Agents / Q000494:pharmacology*; D016716:PC12 Cells; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2; D051381:Rats; D012717:Sesquiterpenes / Q000494:pharmacology*; D051028:bcl-2-Associated X Protein
D000470:Alkaloids; C494828:Bax protein, rat; D018696:Neuroprotective Agents; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D012717:Sesquiterpenes; D051028:bcl-2-Associated X Protein; C050426:huperzine A