title: Biosynthesis of 2-methyl-3-buten-2-ol emitted from needles of Pinus ponderosa via the non-mevalonate DOXP/MEP pathway of isoprenoid formation.
abstract: The volatile hemiterpene 2-methyl-3-buten-2-ol (MBO) is emitted from the needles of several pine species from the Western United States and contributes to ozone formation in the atmosphere. It is synthesised enzymatically from dimethylallyl diphosphate (DMAPP). We show here that needles of Pinus ponderosa Laws. incorporated [1-2H1]-1-deoxy-D-xylulose (d-DOX) into the emitted MBO, but not D,L-[2-13C]mevalonic acid lactone. Furthermore, MBO emission was inhibited by fosmidomycin, a specific inhibitor of the second enzyme of the mevalonate-independent pathway of isopentenyl diphosphate and DMAPP formation, i.e. the 1-deoxy-D-xylulose 5-phosphate/2-C-methyl-D-erythritol 4-phosphate (DOXP/MEP) pathway. We thus prove that MBO emitted from needles of P. ponderosa is primarily formed via the DOXP/MEP pathway.
authors.affiliation: Botanisches Institut II der Universität Karlsruhe, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Zeidler
authors.forename: H K
authors.initials: HK
authors.lastname: Lichtenthaler
mesh_terms: D045782:Hemiterpenes; D008798:Mevalonic Acid / Q000031:analogs & derivatives* / Q000378:metabolism; D009943:Organophosphorus Compounds / Q000378:metabolism; D000439:Pentanols / Q000378:metabolism*; D028223:Pinus / Q000378:metabolism*; D041604:Pinus ponderosa; D013729:Terpenes / Q000378:metabolism*; D014996:Xylulose / Q000031:analogs & derivatives* / Q000378:metabolism
chemical_list: D045782:Hemiterpenes; D009943:Organophosphorus Compounds; D000439:Pentanols; D013729:Terpenes; C004809:isopentenyl pyrophosphate; C043060:3,3-dimethylallyl pyrophosphate; D014996:Xylulose; C029724:1-deoxy-2-pentulose; C015367:mevalonolactone; D008798:Mevalonic Acid; C027250:3-hydroxy-3-methylbutene