A genetic look at the active site of RNA polymerase III.
rpc160-112, a mutant of the RNA polymerase III active site, is corrected in vivo by six second-site mutants obtained by random mutagenesis. These mutants introduce single-site amino acid replacements at the two large subunits of the enzyme. The mutated motifs are conserved in RNA polymerases I and II and, for some of them, in the bacterial enzyme, thus delineating key elements of the active site in eukaryotic RNA polymerases.
Rozenfeld
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Service de Biochimie & Génétique Moléculaire, CEA/Saclay. Bât. 142, F-91191 Gif/Yvette, France.
Thuriaux
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D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D001419:Bacteria / Q000201:enzymology; D001665:Binding Sites; D017124:Conserved Sequence; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016296:Mutagenesis; D017434:Protein Structure, Tertiary; D012313:RNA / Q000096:biosynthesis; D012320:RNA Polymerase III / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D012260:Ribonucleases / Q000378:metabolism; D016415:Sequence Alignment; D013489:Suppression, Genetic; D015003:Yeasts / Q000201:enzymology
D012313:RNA; D012320:RNA Polymerase III; D012260:Ribonucleases