title: Evidence for the involvement of nitric oxide in the control of steroid secretion by the frog adrenal gland.
abstract: Nitric oxide (NO) has been found to modulate the response of rat, bovine and human adrenocortical cells to corticotropic factors. The aim of the present study was to investigate the possible involvement of NO in the control of corticosteroid secretion in the frog Rana ridibunda. Histochemical studies using the NADPH-diaphorase reaction and immunohistochemical labeling with antibodies against NO synthase (NOS) revealed that NOS is exclusively expressed in chromaffin cells. The NO donor sodium nitroprusside (SNP) and the NO synthase inhibitor Nw-nitro-L-arginine (L-NO(2)Arg) did not modify the spontaneous production of corticosterone and aldosterone by perifused adrenal slices. Similarly, L-NO(2)Arg had no effect on the secretory responses induced by ACTH, angiotensin II (AII) and endothelin-1 (ET-1). In contrast, SNP significantly inhibited the stimulatory effects of ACTH, AII and ET-1 on corticosterone and aldosterone secretion. These data provide the first evidence for a modulatory role of NO on adrenocortical cell activity in amphibians.
authors.affiliation: European Institute for Peptide Research (IFRMP 23), Laboratory of Cellular and Molecular Neuroendocrinology, INSERM U 413, UA CNRS, University of Rouen, 76821, Mont-Saint-Aignan, France.
authors.forename: F
authors.initials: F
authors.lastname: Cartier
authors.forename: J L
authors.initials: JL
authors.lastname: Do-Rego
authors.forename: I
authors.initials: I
authors.lastname: Remy-Jouet
authors.forename: A
authors.initials: A
authors.lastname: Fournier
authors.forename: H
authors.initials: H
authors.lastname: Vaudry
authors.forename: C
authors.initials: C
authors.lastname: Delarue
mesh_terms: D000311:Adrenal Glands / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology; D000450:Aldosterone / Q000378:metabolism; D000804:Angiotensin II / Q000494:pharmacology; D000818:Animals; D001001:Anura; D002478:Cells, Cultured; D003345:Corticosterone / Q000378:metabolism; D004347:Drug Interactions; D019332:Endothelin-1 / Q000494:pharmacology; D009569:Nitric Oxide / Q000502:physiology*; D020030:Nitric Oxide Donors / Q000494:pharmacology; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors / Q000378:metabolism; D052248:Nitric Oxide Synthase Type I; D019335:Nitroarginine / Q000494:pharmacology; D009599:Nitroprusside / Q000494:pharmacology; D013256:Steroids / Q000096:biosynthesis* / Q000378:metabolism
chemical_list: D019332:Endothelin-1; D020030:Nitric Oxide Donors; D013256:Steroids; D000804:Angiotensin II; D009599:Nitroprusside; D019335:Nitroarginine; D009569:Nitric Oxide; D000450:Aldosterone; D000324:Adrenocorticotropic Hormone; D019001:Nitric Oxide Synthase; D052248:Nitric Oxide Synthase Type I; D003345:Corticosterone