title: Liquid chromatographic methods for chloral hydrate determination.
abstract: Liquid chromatographic methods, based on reversed-phase (RP) and anion-exchange mechanisms, have been developed for chloral hydrate determination. Both methods are preceeded by derivatization of chloral hydrate. For RP separations, different reagents [namely dansylhydrazine and o-(4-nitrobenzyl)hydroxylamine] have been studied, but the best results have been achieved using 1,2-benzenedithiol with UV detection at 220 nm. The anion-exchange method is based on derivatization with NaOH to form sodium formate that is then analyzed by anion-exchange, with suppressed conductivity detection. Derivatization conditions were optimized in order to reach the best yield of reaction. The optimization of the procedure allowed to determine chloral hydrate with detection limits as low as 0.2 microg/l with good linearity and reproducibility. The anion-exchange method was also applied for chloral hydrate determination in a drinking water sample. A preconcentration procedure has also been studied.
authors.affiliation: Department of Analytical Chemistry, University of Turin, Italy.
authors.forename: M C
authors.initials: MC
authors.lastname: Bruzzoniti
authors.forename: E
authors.initials: E
authors.lastname: Mentasti
authors.forename: C
authors.initials: C
authors.lastname: Sarzanini
authors.forename: E
authors.initials: E
authors.lastname: Tarasco
mesh_terms: D002697:Chloral Hydrate / Q000032:analysis*; D002852:Chromatography, Ion Exchange / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D014881:Water Supply / Q000032:analysis
chemical_list: D002697:Chloral Hydrate