Utilization of marker-assisted congenics to map two blood pressure quantitative trait loci in Dahl rats.
A broad Chromosome (Chr) 10 region of the Dahl salt-sensitive (S) rat was shown by linkage and the use of congenic strains to contain a blood pressure (BP) quantitative trait locus (QTL). To further narrow down the region harboring the QTL, four congenic strains carrying smaller segments were made by replacing various segments of the S rats with the homologous segments of the Lewis (LEW) rats. The construction of these congenic strains was facilitated by a genome-wide marker screening. One congenic strain, assigned as S.L4, showed a BP-lowering effect, and the region harboring a BP QTL, designated QTL1, is localized to a segment of about 15 cM. Two other strains, assigned as S.L2 and S.L5, contained an overlapping segment, and both showed a BP-lowering effect. In contrast, the fourth congenic strain, assigned as S.L1, contained a smaller and shared fragment with S.L2 and S.L5, but it did not have a BP-lowering effect. Deducing from the segment in common in S.L2 and S.L5, and not shared between S.L1 and both congenic strains S.L2 and S.L5, the region harboring a QTL, designated as QTL2, was narrowed to about 12 cM. The current work showed the general applicability of the 'speed congenic' approach to map and fine-map BP QTL.
Deng
A Y
AY
Research Centre-CHUM, Hôtel Dieu, 7-132 Pavillon Jeanne Mance, 3840 rue St. Urbain, Montréal, Québec, H2W 1T8, Canada. alan.deng@umontreal.ca
Dutil
J
J
Sivo
Z
Z
D000818:Animals; D020296:Animals, Congenic; D001794:Blood Pressure / Q000235:genetics*; D002874:Chromosome Mapping; D002875:Chromosomes / Q000235:genetics*; D005819:Genetic Markers / Q000235:genetics; D019655:Quantitative Trait, Heritable; D051381:Rats; D020303:Rats, Inbred Dahl
D005819:Genetic Markers