Identification of a novel gene, CDCP1, overexpressed in human colorectal cancer.
We report the identification of a novel human tumor associated gene, CDCP1 (Cub Domain Containing Protein), which was identified using representational difference analysis and cDNA chip technology. The gene consists of eight exons, the upstream region of which neither contains a TATA- nor a CCAAT-box. However, a CpG island is located around the transcription start, which is found in approximately 60% of known genes. The CDCP1 gene was mapped to chromosome 3p21-p23 by fluorescence in situ hybridization. For expression profiling real time quantitative RT--PCR was performed using cell lines and laser capture microdissected colon cancer biopsies. CDCP1 mRNA is approximately 6 kb and highly overexpressed in human colon cancer and lung cancer. CDCP1 represents a putative transmembrane protein, containing three CUB domains in the extracellular part most likely involved in cell adhesion or interacting with the extracellular matrix.
Scherl-Mostageer
M
M
Research and Development, Department of Exploratory Research, Boehringer Ingelheim Austria, Vienna 1121 Austria.
Sommergruber
W
W
Abseher
R
R
Hauptmann
R
R
Ambros
P
P
Schweifer
N
N
D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism; D000595:Amino Acid Sequence; D015703:Antigens, CD; D000951:Antigens, Neoplasm; D001483:Base Sequence; D015815:Cell Adhesion Molecules / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D002893:Chromosomes, Human, Pair 3; D003001:Cloning, Molecular; D015179:Colorectal Neoplasms / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins; D020411:Oligonucleotide Array Sequence Analysis; D017434:Protein Structure, Tertiary; D012334:RNA, Neoplasm / Q000096:biosynthesis; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
D015703:Antigens, CD; D000951:Antigens, Neoplasm; C432044:CDCP1 protein, human; D015815:Cell Adhesion Molecules; D008565:Membrane Proteins; D009363:Neoplasm Proteins; D012334:RNA, Neoplasm