title: Effects of an angelica extract on human erythrocyte aggregation, deformation and osmotic fragility.
abstract: In Chinese traditional medicine, angelica is widely used for its known clinical effects of ameliorating blood microcirculation. But the mechanism of these beneficial effects still remains unclear. In this work the rheological behaviour of human erythrocytes treated by angelica was studied in vitro. Normal RBCs incubated with an angelica extract at different concentrations (5, 10 or 20 mg/ml) for 60 min at 37 degrees C and then their aggregation, deformation and osmotic fragility were measured with different recently developed optical techniques, namely Erythroaggregometer (Regulest, Florange, France), LORCA (Mechatronics, Amsterdam) and Fragilimeter (Regulest, Florange, France). Experimental results show that angelica (20 mg/ml) significantly decreased normal RBCs' aggregation speed (p<0.01) and could inhibit the hyperaggregability caused by dextran 500. However, the strength of normal RBCs aggregates were not influenced by angelica. When a calcium ionophore A23187 (1.9 microM) was used to harden cell membrane, angelica (20 mg/ml) could significantly (p<0.01) protect erythrocytes against the loss of their deformability even it had no effects on normal RBCs deformation. Finally angelica (5 and 10 mg/ml) decreased significantly (p<0.01) normal RBCs osmotic fragility. In conclusion angelica plays a rheologically active role on human erythrocytes, and this study suggests a possible mechanism for angelica's positive effects against certain cardiovascular diseases.
authors.affiliation: Group Cell and Tissue Mechanics and Engineering, LEMTA - UMR 7563 CNRS/INPL/UHP, 54500 Vandoeuvre-lès-Nancy, France. xwang@ensem.inpl-nancy.fr
authors.forename: X
authors.initials: X
authors.lastname: Wang
authors.forename: L
authors.initials: L
authors.lastname: Wei
authors.forename: J P
authors.initials: JP
authors.lastname: Ouyang
authors.forename: S
authors.initials: S
authors.lastname: Muller
authors.forename: M
authors.initials: M
authors.lastname: Gentils
authors.forename: G
authors.initials: G
authors.lastname: Cauchois
authors.forename: J F
authors.initials: JF
authors.lastname: Stoltz
mesh_terms: D019659:Asteraceae; D002681:China; D004903:Erythrocyte Aggregation / Q000187:drug effects*; D004907:Erythrocyte Deformability / Q000187:drug effects*; D004912:Erythrocytes / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D066298:In Vitro Techniques; D008516:Medicine, Chinese Traditional; D009996:Osmotic Fragility / Q000187:drug effects*; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology*; D010946:Plants, Medicinal
chemical_list: D010936:Plant Extracts