Rapid micro-assay of camptothecin in Camptotheca acuminata.
A micro-assay has been developed to extract and rapidly quantify the anticancer alkaloid, camptothecin (CPT), from two leaf disks of Camptotheca acuminata Decaisne (Nyssaceae). This assay utilizes thin-layer chromatography in conjunction with fluorescence imaging to obtain reproducible measurements in the nanogram range. A large number of trees can be screened using this procedure to identify high producers of CPT in a relatively short period of time.
Nolte
B A
BA
Lineberger
R D
RD
Reed
D W
DW
Rumpho
M E
ME
D000470:Alkaloids / Q000032:analysis* / Q000302:isolation & purification*; D000972:Antineoplastic Agents, Phytogenic / Q000032:analysis* / Q000302:isolation & purification*; D002166:Camptothecin / Q000032:analysis* / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D004264:DNA Topoisomerases, Type I / Q000378:metabolism; D008752:Methylene Chloride; D010936:Plant Extracts / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*
D000470:Alkaloids; D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts; D008752:Methylene Chloride; D004264:DNA Topoisomerases, Type I; D002166:Camptothecin