Determination of periodontal ligament cell viability in long shelf-life milk.
The purpose of this study was to determine the ability of long shelf-life milk to serve as a temporary storage medium for the maintenance of periodontal ligament (PDL) cell viability on avulsed teeth. PDL cells were plated onto 24-well culture plates and allowed to attach for 24 h. Minimal Essential Medium was replaced with regular pasteurized milk (refrigerated milk), long shelf-life milk (Parmalat), or Save-A-Tooth. Tap water served as the negative control, and Minimal Essential Medium served as the positive control. The tissue culture plates were incubated at 37 degrees C for 1, 2, 4, or 8 h. Cell viability was determined using a cell proliferation assay (CellTiter 96 AQ Assay) and absorbance read at 490 nm. ANOVA indicated that all media performed significantly better than tap water at all time periods. At 8 h, PDL cell viability in regular pasteurized milk and long shelf-life milk were significantly greater than in Save-A-Tooth (p < or = 0.001). There was no significant difference between regular pasteurized milk and long shelf-life milk at any time period. These results suggest that long shelf-life milk, which has the advantage of not requiring refrigeration, is as effective a storage medium for avulsed teeth as regular pasteurized milk and more effective than Save-A-Tooth.
Marino
T G
TG
U.S. Army Dental Corps, Fort Gordon, GA 30905, USA.
West
L A
LA
Liewehr
F R
FR
Mailhot
J M
JM
Buxton
T B
TB
Runner
R R
RR
McPherson
J C
JC
D000704:Analysis of Variance; D000818:Animals; D002448:Cell Adhesion; D002452:Cell Count; D002455:Cell Division; D002470:Cell Survival; D002478:Cells, Cultured; D003470:Culture Media / Q000145:classification; D003584:Cytological Techniques; D005524:Food Technology; D006801:Humans; D008892:Milk; D010513:Periodontal Ligament / Q000166:cytology*; D013223:Statistics as Topic; D013997:Time Factors; D014084:Tooth Avulsion / Q000473:pathology; D014867:Water
D003470:Culture Media; D014867:Water