title: Increased erythritol production in fed-batch cultures of Torula sp. by controlling glucose concentration.
abstract: The effect of glucose concentration on erythritol production by Torula sp. was investigated. The maximum volumetric productivity of erythritol was obtained at an initial glucose concentration of 300 g l(-1) in batch culture. The volumetric productivity was maximal at a controlled glucose concentration of 225 g l(-1), reducing the lag time of the erythritol production. A fed-batch culture was established with an initial glucose concentration of 300 g l(-1) and with a controlled glucose concentration of 225 g l(-1) in medium containing phytic acid as a phosphate source. In this fed-batch culture, a final erythritol production of 192 g l(-1) was obtained from 400 g l(-1) glucose in 88 h. This corresponded to a volumetric productivity of 2.26 g l(-1) h(-1) and a 48% yield.
authors.affiliation: Department of Bioscience and Biotechnology, Sejong University, 98 Kunja-Dong, Kwangjin-Ku, Seoul 143-747, South Korea.
authors.forename: D K
authors.initials: DK
authors.lastname: Oh
authors.forename: C H
authors.initials: CH
authors.lastname: Cho
authors.forename: J K
authors.initials: JK
authors.lastname: Lee
authors.forename: S Y
authors.initials: SY
authors.lastname: Kim
mesh_terms: D002455:Cell Division / Q000187:drug effects; D003454:Cryptococcus / Q000145:classification / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D004896:Erythritol / Q000096:biosynthesis*; D005285:Fermentation; D005947:Glucose / Q000378:metabolism*; D007700:Kinetics; D010710:Phosphates / Q000378:metabolism; D010833:Phytic Acid / Q000378:metabolism / Q000494:pharmacology; D013997:Time Factors
chemical_list: D010710:Phosphates; D010833:Phytic Acid; D005947:Glucose; D004896:Erythritol