An enzymatic method for the detection of human serum albumin.
Albumin is the most abundant protein in human serum. A dye-binding method is commonly used in clinical laboratories for its estimation using different types of dyes. However, all these dye methods were interfered by a variety of compounds. Here we present a method for the detection of albumin in human serum and other biological fluids. The principle is based on the fact that lactate dehydrogenase isoenzyme-5 (LDH-5) binds specifically to Dextran-Blue (DB). Albumin inhibits the binding of LDH-5 with DB. Absence of LDH activity in DB fraction after gel filtration indicates the presence of albumin in sample and vice versa.
Javed
M U
MU
Department of Biochemistry, The Aga Khan University Medical College, Karachi, Pakistan. masoodjaved@hotmail.com
Waqar
S N
SN
D002624:Chemistry, Clinical / Q000379:methods*; D002850:Chromatography, Gel; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D000080864:Lactate Dehydrogenase 5; D011485:Protein Binding; D012685:Sepharose / Q000737:chemistry; D012709:Serum Albumin / Q000032:analysis*
D007527:Isoenzymes; D012709:Serum Albumin; D012685:Sepharose; D007770:L-Lactate Dehydrogenase; D000080864:Lactate Dehydrogenase 5