[Effect of reduced and oxidized glutathione on physico-chemical properties of erythrocyte membranes].
The parameters describing the structural and functional state of membranes depending on the level of reduced glutathione in erythrocytes were studied. It was shown, that the decrease in the concentration of reduced intracellular glutathione in erythrocytes upon metabolic depletion (prolonged incubation of cells at 37 degrees C in the absence of glucose) or a rapid irreversible depletion of glutathione with 1-chloro-2,4-dinitrobenzene enhances lipid peroxidation processes in membranes, inhibits the membrane-bound NAD.H methemoglobin reductase activity and decreases the intensity of 1,6-diphenyl-1,3,5-hexatrien fluorescence. The data obtained suggest that the depletion of reduced intracellular glutathione causes changes in the physicochemical state of the erythrocyte membrane: the accumulation of lipid peroxidation products, changes in the physical state of lipid bilayer and the inhibition of membrane-bound NAD.H-methemoglobin reductase activity.
Kozlova
N M
NM
Institute of Photobiology, Belarussian Academy of Sciences, ul. Academicheskaya 27, Minsk, 220072 Belarus.
Slobozhanina
E I
EI
Antonovich
A N
AN
Luk'ianenko
L M
LM
Chernitskiĭ
E A
EA
D003470:Culture Media; D004137:Dinitrochlorobenzene / Q000494:pharmacology; D004161:Diphenylhexatriene; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000378:metabolism*; D005456:Fluorescent Dyes; D005947:Glucose / Q000172:deficiency; D005978:Glutathione / Q000378:metabolism*; D019803:Glutathione Disulfide / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D015227:Lipid Peroxidation
D003470:Culture Media; D004137:Dinitrochlorobenzene; D005456:Fluorescent Dyes; D004161:Diphenylhexatriene; D005978:Glutathione; D005947:Glucose; D019803:Glutathione Disulfide