title: Expression of the DX5 antigen on CD8+ T cells is associated with activation and subsequent cell death or memory during influenza virus infection.
abstract: The antigen recognized by the DX5 antibody (DX5 antigen) is expressed on all murine NK cells. In the present study we found that a proportion of CD8+ T cells (approximately 5%) also express the DX5 antigen in uninfected mice, and that numbers of CD8+ T cells expressing DX5 are significantly higher in the lungs of influenza virus-infected mice representing up to 50% of all CD8+ T cells on day 10 post infection. The expression of the DX5 antigen on CD8+ T cells was associated with a memory phenotype in uninfected C57BL/6 mice and with an activation phenotype during influenza virus infection. Interestingly, when lymphocytes were isolated from lungs of influenza virus-infected mice on day 10 post infection and adoptively transferred into recombination activating gene-1 (RAG1)-deficient mice, CD8+DX5+ cells could not be recovered from the recipient mice 2 days later. Moreover, CD8+DX5+ cells were not detected when lung cells were removed from day 10 influenza virus-infected mice and cultured in vitro for 2 days. However, CD8+DX5+ cells could be detected when apoptosis inhibitors were added to these cultures, suggesting that the CD8+DX5+ cells underwent apoptosis during cell culture. Furthermore, almost all DX5 expressing CD8+ cells from lungs of mice on day 10 post influenza virus infection stained positively with Annexin-V. Taken together, the data suggest that CD8+ T cells expressing DX5 are associated with an activation/memory phenotype and are biased towards apoptosis.
authors.affiliation: Microbiology and Tumor Biology Center, Karolinska Institutet, Stockholm, Sweden.
authors.forename: T
authors.initials: T
authors.lastname: Kambayashi
authors.forename: E
authors.initials: E
authors.lastname: Assarsson
authors.forename: B J
authors.initials: BJ
authors.lastname: Chambers
authors.forename: H G
authors.initials: HG
authors.lastname: Ljunggren
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D000906:Antibodies / Q000276:immunology; D000918:Antibody Specificity; D000941:Antigens / Q000235:genetics / Q000276:immunology*; D017209:Apoptosis; D015415:Biomarkers / Q000032:analysis; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D017690:Cell Transplantation; D002478:Cells, Cultured; D005434:Flow Cytometry; D017353:Gene Deletion; D016654:Genes, RAG-1 / Q000235:genetics; D015395:Histocompatibility Antigens Class I / Q000276:immunology; D007156:Immunologic Memory; D009980:Influenza A virus / Q000276:immunology*; D008168:Lung / Q000276:immunology / Q000821:virology; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009976:Orthomyxoviridae Infections / Q000276:immunology*; D015854:Up-Regulation
chemical_list: D000906:Antibodies; D000941:Antigens; D015415:Biomarkers; D015395:Histocompatibility Antigens Class I