[Effect of gamma-plant preparation on production of TNF-alpha, IL-1 beta, and IL-6 in human peripheral blood mononuclear cells in vitro].
The effect of a new medicine gamma-plant has antiinflammatory activity on human peripheral blood mononuclear cells obtained from healthy donors was studied. Its ability to influence TNF, IL-1 and IL-6 production via mononuclear cells was determined. Lymphokines spontaneous production and lymphokines productions by cells stimulated by LPS was studied. IL-1 content was defined with IL-1 sensitive cell line D10G4.1 while IL-6 content with IL-6 dependent heterohybridoma D6C8. TNF activity in supernatants was determined as lysis grade of TNF-sensitive cells of mouse fibrosarcoma L-929. It was shown that gamma-plant acted as stimulator when IL-1 production was low and as inhibitor when it was high. Stimulation of IL-6 production induced with LPS was achieved by low gamma-plant doses while for stimulation of spontaneous production higher doses were required. After nonstimulated LPS peripheral blood cells were treated with gamma-plant statistically sensible stimulated TNF production was observed apart from TNF synthesis level in a control group. The TNF production inhibition could never be demonstrated in subjects that showed in control cultures stimulated with LPS a level of TNF production lower than 100 pg/ml. In four of the five high TNF producers the cytokine release inhibition in LPS stimulated cultures has been observed. It is likely that the mild stimulation of the proinflammatory cytokine system has taken place. For example, simultaneously with stimulation of cytokine production the circulating IL-1 receptor antagonist and/or soluble TNF receptor may be induced.
Chekanovskaia
L A
LA
Institute of Molecular Genetics of Russian Academy of Sciences, Kurchatov sq. 2, 123182, Russia, Moscow.
Generalov
A V
AV
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D006023:Glycoproteins / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000096:biosynthesis*; D015850:Interleukin-6 / Q000097:blood*; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000378:metabolism; D010944:Plants / Q000737:chemistry; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
D000276:Adjuvants, Immunologic; D000894:Anti-Inflammatory Agents, Non-Steroidal; D006023:Glycoproteins; D007375:Interleukin-1; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha