title: Pertussis toxin-induced hyperacute autoimmune encephalomyelitis in Lewis rats is correlated with increased expression of inducible nitric oxide synthase and tumor necrosis factor alpha.
abstract: The involvement of inducible nitric oxide synthase (iNOS) and tumor necrosis factor alpha (TNF-alpha), which have diverse roles in the progression of autoimmune disease models, was studied in pertussis toxin (PT)-induced hyperacute experimental autoimmune encephalomyelitis (EAE) in Lewis rats. The expression of TNF-alpha mRNA (increased 5-fold, P<0.01) and iNOS protein (3-fold, P<0.01) was much greater in the spinal cords with PT(+) EAE at the peak stage of EAE than in those with PT(-) EAE, as shown by competitive PCR and Western blot analysis, respectively. Immunohistochemistry showed that the majority of ED1-positive macrophages in EAE lesions contained iNOS, and that there were many more iNOS-positive cells in the CNS lesions of PT(+) rats than in those of PT(-) rats. These findings suggest that PT-induced hyperacute EAE is partly mediated by the enhanced expression of iNOS and TNF-alpha in the early stages of rat EAE.
authors.affiliation: Department of Veterinary Medicine, Institute for Life Science, Brain Korea 21, SHRC, Cheju National University, 690-756, Jeju, South Korea.
authors.forename: M
authors.initials: M
authors.lastname: Ahn
authors.forename: J
authors.initials: J
authors.lastname: Kang
authors.forename: Y
authors.initials: Y
authors.lastname: Lee
authors.forename: K
authors.initials: K
authors.lastname: Riu
authors.forename: Y
authors.initials: Y
authors.lastname: Kim
authors.forename: Y
authors.initials: Y
authors.lastname: Jee
authors.forename: Y
authors.initials: Y
authors.lastname: Matsumoto
authors.forename: T
authors.initials: T
authors.lastname: Shin
mesh_terms: D000818:Animals; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000139:chemically induced / Q000201:enzymology* / Q000503:physiopathology; D005260:Female; D008264:Macrophages / Q000166:cytology / Q000201:enzymology; D009103:Multiple Sclerosis / Q000201:enzymology* / Q000473:pathology / Q000503:physiopathology; D019001:Nitric Oxide Synthase / Q000187:drug effects / Q000378:metabolism*; D037342:Pertussis Toxin; D051381:Rats; D011917:Rats, Inbred Lew; D013116:Spinal Cord / Q000187:drug effects / Q000201:enzymology* / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000187:drug effects / Q000378:metabolism*; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
chemical_list: D014409:Tumor Necrosis Factor-alpha; D010566:Virulence Factors, Bordetella; D019001:Nitric Oxide Synthase; D037342:Pertussis Toxin