Optimization of production and downstream processing of the almond beta-glucosidase-mediated glucosylation of glycerol.
This article describes the synthesis of glyceryl glucoside from glycerol and glucose with almond beta-glucosidase as the catalyst. A yield of 54% (0.45 mmol/g) was obtained. The influence of the enzyme stability, the water concentration, and the water activity on the glucoside yield were determined. A molar fraction-based equilibrium constant of 2.4 +/- 0.6 was found, with which the glucoside yield could be calculated for all possible combinations of initial substrate and water fractions in the reaction mixture. A model was used to optimize the glucoside yield while minimizing one of the substrate concentrations at equilibrium. This straightforward model gives a good prediction of the measured glucoside yield, according to a parity plot.
de Roode
M
M
Department of Food Technology and Nutritional Sciences, Food and Bioprocess Engineering Group, Wageningen University, Bomenweg 2, 6703 HD Wageningen, The Netherlands.
Peters
S W
SW
Franssen
M C
MC
van der Padt
A D
AD
de Groot
A
A
Boom
R M
RM
D005947:Glucose / Q000378:metabolism; D005960:Glucosides / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism*; D006031:Glycosylation; D008956:Models, Chemical; D001617:beta-Glucosidase / Q000378:metabolism*
D005960:Glucosides; D001617:beta-Glucosidase; D005947:Glucose; D005990:Glycerol