A small family of novel CuZn-superoxide dismutases with high isoelectric points in hybrid aspen.
Several CuZn-superoxide dismutases (SODs; EC 1.15.1.1) were cloned from hybrid aspen (Populus tremula L. x tremuloides Michx.). Two of the cloned genes encode representatives of a novel type of CuZn-SOD and we named it HipI-SOD because of its high isoelectric point (> or =9). The SODs were cloned by screening a cDNA library with a probe based on a Scots pine (Pinus sylvestris L.) CuZn-SOD that is predominantly located extracellularly. The expression pattern of HipI-SOD was examined using a Northern blot technique and compared with the expression patterns of cytosolic and chloroplastic SODs. Distinct expression patterns were observed for the three types of CuZn-SOD, with HipI-SODs showing strong expression in apical tissues. Southern blots as well as protein analysis suggest that these novel HipI-SODs belong to a small gene family, one member of which might be monomeric.
Schinkel
H
H
Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, Umeå.
Hertzberg
M
M
Wingsle
G
G
D000595:Amino Acid Sequence; D002678:Chimera; D018076:DNA, Complementary; D018744:DNA, Plant; D018506:Gene Expression Regulation, Plant; D007526:Isoelectric Point; D008969:Molecular Sequence Data; D031308:Salicaceae / Q000201:enzymology / Q000235:genetics*; D013482:Superoxide Dismutase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
D018076:DNA, Complementary; D018744:DNA, Plant; D013482:Superoxide Dismutase