[Evaluation of brain ischemia by mitochondrial respiration: experimental model].
Brain ischemia occurs in several diseases. One of the critical factors for recovery of patients is the duration of the ischemic process. Brain activity depends on the energetic supply, it suggests that the study of mitochondrial function can be useful for evaluation of neuronal damage. The purpose of the present research was to study the mitochondrial respiration by occlusion of the left middle cerebral artery by intraluminal suture technique. Adults Wistar rats were subdivided in 4 groups: control, 15, 30 and 60 minutes of occlusion. Results showed that there was no significant difference between the group of 15 minutes and the control group. The group of 30 minutes had significant decrease of state III of mitochondrial respiration compared with control group. The group of 60 minutes had significant decrease in state III and IV of mitochondrial respiration compared with control group. Mitochondrial respiration allowed an early and effective evaluation of focal ischemic process of the rat brain.
Carlotti
C G
CG
Departamento de Cirurgia, Faculdade de Medicina de Ribeirão Preto, Universidade São Paulo, Ribeirão Preto, SP, Brasil. carlotti@fmrp.usp.br
Colli
B O
BO
Kazuo
J Y
JY
D000818:Animals; D002545:Brain Ischemia / Q000503:physiopathology*; D019069:Cell Respiration / Q000502:physiology; D008297:Male; D008928:Mitochondria / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors