Type I IFN modulates the immune response induced by DNA vaccination to pseudorabies virus glycoprotein C.
DNA vaccines have the capacity to induce strong Th1-biased immune responses that are of major importance to providing protection against intracellular pathogens. In the present study we have focused on the role played by type I IFN in immune responses induced after DNA vaccination. Mice lacking the IFNAR1 chain of the type I IFN receptor (IFNAR K/O mice) were immunized with a plasmid encoding glycoprotein C of pseudorabies virus (PRV-gC). After DNA vaccination, wild-type (WT) mice showed features characteristic of Th1 immune responses, such as high IgG2a:IgG1 anti-PRV Ab ratio and antigen-specific IFN-gamma production by spleen cells. In contrast, IFNAR K/O mice showed a significantly lower IgG2a:IgG1 Ab ratio and IFN-gamma production. In addition, the percentage of CD8(+) and B lymph-node cells expressing CD69 after PRV-gC DNA vaccination was lower in IFNAR K/O than in WT mice. These results support a major role played by type I IFN in shaping Th1 immune responses after DNA vaccination. Codelivery of plasmids encoding IL-12 and IL-18 along with the plasmid encoding PRV-gC restored Th1 responses in IFNAR K/O mice.
Tudor
D
D
Institut National de la Recherche Agronomique (INRA), Unité de Virologie et d'Immunologie Moléculaires, Jouy-en-Josas, 78350, France.
Riffault
S
S
Carrat
C
C
Lefèvre
F
F
Bernoin
M
M
Charley
B
B
D000818:Animals; D011558:Herpesvirus 1, Suid / Q000276:immunology*; D007109:Immunity; D007372:Interferons / Q000276:immunology*; D051379:Mice; D011557:Pseudorabies / Q000276:immunology* / Q000517:prevention & control; D022421:Pseudorabies Vaccines / Q000276:immunology*; D017471:Receptors, Interferon / Q000276:immunology; D014611:Vaccination; D014759:Viral Envelope Proteins / Q000276:immunology
D022421:Pseudorabies Vaccines; D017471:Receptors, Interferon; D014759:Viral Envelope Proteins; C042966:pseudorabies virus glycoproteins; D007372:Interferons