Ozone quenching properties of isoprene and its antioxidant role in leaves.
Isoprene is formed in and emitted by plants and the reason for this apparent carbon waste is still unclear. It has been proposed that isoprene stabilizes cell and particularly chloroplast thylakoid membranes. We tested if membrane stabilization or isoprene reactivity with ozone induces protection against acute ozone exposures. The reduction of visible, physiological, anatomical, and ultrastructural (chloroplast) damage shows that clones of plants sensitive to ozone and unable to emit isoprene become resistant to acute and short exposure to ozone if they are fumigated with exogenous isoprene, and that isoprene-emitting plants that are sensitive to ozone do not suffer damage when exposed to ozone. Isoprene-induced ozone resistance is associated with the maintenance of photochemical efficiency and with a low energy dissipation, as indicated by fluorescence quenching. This suggests that isoprene effectively stabilizes thylakoid membranes. However, when isoprene reacts with ozone within the leaves or in a humid atmosphere, it quenches the ozone concentration to levels that are less or non-toxic for plants. Thus, protection from ozone in plants fumigated with isoprene may be due to a direct ozone quenching rather than to an induced resistance at membrane level. Irrespective of the mechanism, isoprene is one of the most effective antioxidants in plants.
Loreto
F
F
Consiglio Nazionale delle Ricerche, Istituto di Biochimica ed Ecofisiologia Vegetali,Via Salaria Km 29,300, 00016 Monterotondo Scalo, Roma, Italy. franci@mlib.cnr.it
Mannozzi
M
M
Maris
C
C
Nascetti
P
P
Ferranti
F
F
Pasqualini
S
S
D000975:Antioxidants / Q000378:metabolism*; D002070:Butadienes / Q000378:metabolism*; D045782:Hemiterpenes; D010126:Ozone / Q000378:metabolism*; D010420:Pentanes; D018515:Plant Leaves / Q000378:metabolism*; D019685:Rosales; D014197:Trees
D000975:Antioxidants; D002070:Butadienes; D045782:Hemiterpenes; D010420:Pentanes; C005059:isoprene; D010126:Ozone