title: Mitochondrial respiration and triiodothyronine concentration in liver from postpubertal and adult rats.
abstract: The purpose of this study was to investigate the decline in rat liver mitochondria respiration found in adult rats compared to younger ones, and to find a link between this respiratory impairment and a tissue hypothyroidism state. To this end, hepatic concentration and serum levels of triiodothyronine were measured in postpubertal rats (60 days old) and adult rats (180 days old). In addition, in these rats we measured oxidative phosphorylation in homogenate together with coupled and uncoupled respiration in isolated mitochondria using succinate or durohydroquinone as substrate. We found that mitochondria from adult rats consumed less oxygen compared to younger rats due to lower electron transport chain and phosphorylating system activity. In addition, we found that in state 4 condition, mitochondria from adult rats consumed less oxygen than mitochondria from young rats. Finally, we found a decrease in liver triiodothyronine concentration in adult rats. In conclusion, the results of this study show that hepatic mitochondria in adult rats have a decreased ATP synthesis capacity and proton permeability, both consistent with the tissue hypothyroidism found in the liver of adult rats.
authors.affiliation: Department of General and Environmental Physiology, University of Naples FEDERICO II, Italy. susiossa@unina.it
authors.forename: S
authors.initials: S
authors.lastname: Lossa
authors.forename: L
authors.initials: L
authors.lastname: Lionetti
authors.forename: M P
authors.initials: MP
authors.lastname: Mollica
authors.forename: R
authors.initials: R
authors.lastname: Crescenzo
authors.forename: M
authors.initials: M
authors.lastname: Botta
authors.forename: G
authors.initials: G
authors.lastname: Liverini
mesh_terms: D000255:Adenosine Triphosphate / Q000096:biosynthesis; D000375:Aging / Q000378:metabolism*; D000818:Animals; D019069:Cell Respiration / Q000502:physiology; D003574:Cytochrome c Group / Q000378:metabolism; D014450:Electron Transport Complex III / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000378:metabolism; D006873:Hydroquinones / Q000493:pharmacokinetics; D008099:Liver / Q000378:metabolism*; D008297:Male; D008928:Mitochondria / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012402:Rotenone / Q000494:pharmacology; D013385:Succinate Dehydrogenase / Q000378:metabolism; D019802:Succinic Acid / Q000493:pharmacokinetics; D014284:Triiodothyronine / Q000097:blood*; D014475:Uncoupling Agents / Q000494:pharmacology
chemical_list: D003574:Cytochrome c Group; D006873:Hydroquinones; D014475:Uncoupling Agents; D012402:Rotenone; D014284:Triiodothyronine; D000255:Adenosine Triphosphate; D019802:Succinic Acid; D013385:Succinate Dehydrogenase; D003576:Electron Transport Complex IV; D014450:Electron Transport Complex III; C016421:duroquinol