The ferret's vomeronasal organ and accessory olfactory bulb: effect of hormone manipulation in adult males and females.
The male ferret, a carnivore, was recently shown to possess a vomeronasal organ (VNO). We compared the morphology of the VNO and its associated accessory olfactory bulb (AOB) in male and female ferrets that were killed in adulthood. The volume and surface area of the VNO neuroepithelium were similar in adult gonadectomized male and female ferrets regardless of whether they were treated with testosterone propionate (TP) or oil vehicle. An AOB was localized bilaterally in the medial caudal part of the olfactory bulbs of adult ferrets using soybean agglutin binding and immunostaining for luteinizing hormone-releasing hormone and tyrosine hydroxylase as well as Nissl staining of coronal, horizontal, and sagittal brain sections. There was no effect of sex or TP treatment on AOB cell layer volume in adult gonadectomized animals. We found the ferret's AOB to be more medially located and much smaller than previously reported in this species, thus highlighting the importance of using several histochemical markers to characterize this structure in any previously unexamined species. Adult male and female ferrets both have a VNO and an associated AOB. More research is needed to determine what role, if any, this accessory olfactory system plays in mediating behavioral and neuroendocrine responses to pheromones in ferrets of either sex.
Kelliher
K R
KR
Department of Biology, Boston University, Boston, MA 02215, USA. kkell002@umaryland.edu
Baum
M J
MJ
Meredith
M
M
D000818:Animals; D005260:Female; D005289:Ferrets / Q000033:anatomy & histology*; D012739:Gonadal Steroid Hormones / Q000494:pharmacology*; D007987:Gonadotropin-Releasing Hormone / Q000032:analysis; D006651:Histocytochemistry; D037102:Lectins / Q000032:analysis; D008297:Male; D009562:Nissl Bodies; D009830:Olfactory Bulb / Q000033:anatomy & histology* / Q000737:chemistry / Q000187:drug effects; D037121:Plant Lectins; D012727:Sex Characteristics; D030262:Soybean Proteins; D013739:Testosterone / Q000494:pharmacology*; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis; D019147:Vomeronasal Organ / Q000033:anatomy & histology* / Q000737:chemistry / Q000187:drug effects; D010835:Phytohemagglutinins
D012739:Gonadal Steroid Hormones; D007987:Gonadotropin-Releasing Hormone; D037102:Lectins; D037121:Plant Lectins; D030262:Soybean Proteins; D013739:Testosterone; D014446:Tyrosine 3-Monooxygenase; C028824:soybean lectin; D010835:Phytohemagglutinins