title: Crystallization and preliminary X-ray crystallographic analysis of deoxyuridine triphosphate nucleotidohydrolase from Saccharomyces cerevisiae.
abstract: Deoxyuridine triphosphate nucleotidohydrolase (dUTPase) from Saccharomyces cerevisiae is essential for cell viability. It has been overexpressed in Escherichia coli and has been crystallized at 296 K using polyethylene glycol (PEG) 1500 as a precipitant. The crystals belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 59.48, b = 138.54, c = 157.91 A, alpha = beta = gamma = 90 degrees. Two molecules of trimeric dUTPase from S. cerevisiae are present in the asymmetric unit, giving a crystal volume per protein mass (V(M)) of 3.36 A(3) Da(-1) and a solvent content of 63%. The diffraction limit of the crystals could be significantly extended by the crystal-annealing procedure. A set of native data extending to 2.7 A resolution has been collected at 100 K using synchrotron X-rays.
authors.affiliation: School of Chemistry and Molecular Engineering, College of Natural Sciences, Seoul National University, Seoul 151-742, South Korea.
authors.forename: B W
authors.initials: BW
authors.lastname: Han
authors.forename: J Y
authors.initials: JY
authors.lastname: Lee
authors.forename: J K
authors.initials: JK
authors.lastname: Yang
authors.forename: B I
authors.initials: BI
authors.lastname: Lee
authors.forename: S W
authors.initials: SW
authors.lastname: Suh
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D011487:Protein Conformation; D011755:Pyrophosphatases / Q000737:chemistry*; D012441:Saccharomyces cerevisiae / Q000201:enzymology*
chemical_list: D011755:Pyrophosphatases; C020020:dUTP pyrophosphatase