Analytical microscopy observations of rat enterocytes after oral administration of soluble salts of lanthanides, actinides and elements of group III-A of the periodic chart.
The behavior in the intestinal barrier of nine elements (three of the group III-A, four lanthanides and two actinides), absorbed as soluble salts, has been studied by two microanalytical methods: electron probe X-ray micro analysis (EPMA) and secondary ion mass spectrometry (SIMS). It has been shown that the three elements of group III-A, aluminium, gallium and indium; and the four lanthanides, lanthanum, cerium, europium and thulium, are selectively concentrated and precipitated as non-soluble form in enterocytes of proximal part of the intestinal tract. SIMS microscopy has shown that these elements are concentrated as a number of submicroscopic precipitates, most of them localized in the apical part of the duodenum enterocytes, where they are observed from one hour to 48 hr after a single intragastric administration. No precipitate is observed after three days. It is suggested that this mechanism of local concentration limits the diffusion of these elements through the digestive barrier, some of them being toxic and none of them having a recognized physiological role. Additionally, the precipitation in duodenal enterocytes, the life time of which is on the order of 2-3 days, allows the elements absorbed as soluble form to be eliminated as a non-soluble form in the digestive lumen along with the desquamation of the apoptotic enterocytes. The intracytoplasmic localization of the precipitates are supposed to be the lysosomes although no direct evidence could be given here due to the very small sizes of the lysosomes of enterocytes. The same results were not observed with the two studied actinides. After administration of thorium, only some very sparse microprecipitates could be observed in intestinal mucosa and, after administration of uranium, no precipitates were observed with the exception of some in the conjunctive part of the duodenal villi.
Floren
C
C
Service de Gastro-Entérologie, Hĵpital St. Antoine, Paris, France. cflorent@europost.org
Tekaya
L
L
Escaig
F
F
Labejof
L
L
Mouthon
G
G
Galle
P
P
D008671:Actinoid Series Elements / Q000008:administration & dosage / Q000378:metabolism* / Q000493:pharmacokinetics; D000284:Administration, Oral; D000818:Animals; D017209:Apoptosis; D011232:Chemical Precipitation; D004386:Duodenum / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure; D020895:Enterocytes / Q000166:cytology* / Q000378:metabolism* / Q000648:ultrastructure; D007082:Ileum / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure; D007408:Intestinal Absorption; D007583:Jejunum / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure; D028581:Lanthanoid Series Elements / Q000008:administration & dosage / Q000378:metabolism* / Q000493:pharmacokinetics; D008247:Lysosomes / Q000378:metabolism / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D051381:Rats; D017207:Rats, Sprague-Dawley; D012492:Salts / Q000008:administration & dosage / Q000378:metabolism / Q000493:pharmacokinetics; D012995:Solubility; D018629:Spectrometry, Mass, Secondary Ion
D008671:Actinoid Series Elements; D028581:Lanthanoid Series Elements; D012492:Salts