DNA-induced structural changes in the papillomavirus capsid.
Human papillomavirus capsid assembly requires intercapsomeric disulfide bonds between molecules of the major capsid protein L1. Virions isolated from naturally occurring lesions have a higher degree of cross-linking than virus-like particles (VLPs), which have been generated in eukaryotic expression systems. Here we show that DNA encapsidation into VLPs leads to increased cross-linking between L1 molecules comparable to that seen in virions. A higher trypsin resistance, indicating a tighter association of capsomeres through DNA interaction, accompanies this structural change.
Fligge
C
C
Institute for Medical Microbiology and Hygiene, University of Mainz, D-55101 Mainz, Germany.
Schäfer
F
F
Selinka
H C
HC
Sapp
C
C
Sapp
M
M
D004279:DNA, Viral / Q000502:physiology*; D006801:Humans; D027383:Papillomaviridae / Q000502:physiology*; D014771:Virion / Q000502:physiology; D019065:Virus Assembly
D004279:DNA, Viral