title: Dermoscopy as a second step in the diagnosis of doubtful pigmented skin lesions: how great is the risk of missing a melanoma?
abstract: BACKGROUND
  Little is known about the occurrence of false negatives in the diagnosis of melanoma using dermoscopy in clinical practice: in the literature dermoscopy only increased the accuracy of diagnosis of equivocal lesions that were to undergo biopsy anyway.
  
  
  OBJECTIVE
  We collected the 81 lesions clinically diagnosed as probable melanomas by experienced specialists (from a series of 256 pigmented skin lesions submitted to excisional biopsy) and reviewed them for possible false negative results in the diagnosis of melanoma using in vivo dermoscopy and dermoscopy performed on slide images. Both procedures were carried out by the same experienced dermatologists who classified the lesions clinically.
  
  
  RESULTS
  Dermoscopy made on slide images (observers blinded for clinical features of the lesions) yielded three false negative results (91% sensitivity) in a group of 35 histologically confirmed melanomas. In vivo dermoscopy showed better results, with no melanomas missed (100% sensitivity). The frequency of false positive results in the diagnosis of melanoma was 13.5% (11 of 81) with dermoscopy on slide images and 2.5% (two of 81) with in vivo dermoscopy.
  
  
  CONCLUSIONS
  Only in vivo dermoscopy by fully trained dermatologists with both clinical and dermoscopic experience avoids the risk of misclassification of melanomas otherwise correctly classified on clinical grounds.
authors.affiliation: Department of Dermatology, University of Florence, Italy. CARLI@unifi.it
authors.forename: P
authors.initials: P
authors.lastname: Carli
authors.forename: V
authors.initials: V
authors.lastname: De Giorgi
authors.forename: B
authors.initials: B
authors.lastname: Giannotti
mesh_terms: D003880:Dermatology / Q000295:instrumentation; D003937:Diagnosis, Differential; D005188:False Negative Reactions; D006801:Humans; D008545:Melanoma / Q000473:pathology*; D010859:Pigmentation Disorders / Q000473:pathology*; D012680:Sensitivity and Specificity; D012878:Skin Neoplasms / Q000473:pathology*