title: Inhibition of adhesion of food-borne pathogens to Caco-2 cells by Lactobacillus strains.
abstract: OBJECTIVE
  To select adhesive strains among strains of Lactobacillus and to apply them to inhibit adhesion of food-borne pathogens.
  
  
  RESULTS
  Twelve Lactobacillus strains (10 from intestine) were examined for adhesion using Caco-2 cell cultures. The two most adhesive strains, Lactobacillus crispatus JCM 8779 and Lact. reuteri JCM 1081, were used to test antiadhesion activity against enterotoxigenic Escherichia coli, Salmonella typhimurium and Enterococcus faecalis strains. Adhesion of the pathogens was inhibited by both Lactobacillus strains. Adhesion of Ent. faecalis was especially strongly inhibited by JCM 8779. Although antimicrobial activity was not detected in the culture supernatant fluid by agar well diffusion assay, the supernatant fluid obtained from the harvested JCM 8779 cell suspension showed bactericidal activity against Ent. faecalis.
  
  
  CONCLUSIONS
  The strong antiadhesion activity of JCM 8779 against Ent. faecalis appears to be due to the combined effect of both bactericidal activity and competition for attachment site.
  
  
  CONCLUSIONS
  This is the first report that Lact. crispatus produces a bactericidal substance.
authors.affiliation: Department of Biochemistry and Biotechnology, Faculty of Agriculture and Life Science, Hirosaki University, Bunkyocho 3, 036-8561 Hirosaki, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Todoriki
authors.forename: T
authors.initials: T
authors.lastname: Mukai
authors.forename: S
authors.initials: S
authors.lastname: Sato
authors.forename: T
authors.initials: T
authors.lastname: Toba
mesh_terms: D000898:Antibiosis / Q000502:physiology*; D001422:Bacterial Adhesion / Q000502:physiology; D018938:Caco-2 Cells / Q000382:microbiology* / Q000502:physiology; D018929:Cell Culture Techniques; D002470:Cell Survival; D013293:Enterococcus faecalis / Q000502:physiology; D004926:Escherichia coli / Q000502:physiology; D005506:Food Contamination; D006801:Humans; D007778:Lactobacillus / Q000502:physiology*; D012486:Salmonella typhimurium / Q000502:physiology