[Research of status of provirus HIV DNA in non-progressor and progressor of HIV-1 infected persons].
OBJECTIVE
This study was designed to investigate the different status of provirus HIV DNA in non-progressor and progressor of HIV-1 infected persons.


METHODS
HIV RNA, HIV DNA were detected with long-distance PCR (LD-PCR), reverse transcription PCR (RT-PCR), Southern blot and molecular hybridization from plasma and peripheral blood mononuclear cells (PBMCs), using primers locating between long terminal repeats LTR(U5)-LTR(R) and so on.


RESULTS
9.1 kb band is full-length HIV DNA genome which was present in all templates of 12 progressors. Deleted HIV DNA was observed in 9 of 12 progressors. Both the full-length and defective. provirus HIV DNA was detected in 5 of the 18 non-progressors. Defective HIV DNA was only detected in the other non-progressors.


CONCLUSIONS
Clinical stages are closely related with both the full-length and the defective HIV DNA.
Xu
X
X
Department of Infectious Diseases, the First Clinic Hospital, Beijing Medical University, 100034 Beijing, China.
Chen
L
L
Wang
Q
Q
Chermann
J C
JC
D017931:DNA Primers; D004279:DNA, Viral / Q000032:analysis*; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D016133:Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction
D017931:DNA Primers; D004279:DNA, Viral