Altered Glut4 mRNA levels in specific brain areas of hyperglycemic-hyperinsulinemic rats.
The insulin sensitive glucose transporter Glut4 is expressed in neurons of the brain among which those of hypothalamic nuclei. It has been proposed that this transporter might be involved in the hypothalamic glucose-insulin sensing mechanism and thus in the nervous regulation of metabolism. In order to get further insights into its putative role, Glut4 expression was analyzed by quantitative competitive reverse transcription-polymerase chain reaction, in hypothalamic nuclei of hyperglycemic-hyperinsulinemic (HG-HI) rats, a model characterized by alteration of the autonomic nervous system activity. Glut4 mRNA content was decreased in the lateral hypothalamic area (33%) and arcuate nucleus (27%) but significantly only in the former. It was unchanged in other structures. These results are in favor of an alteration of Glut4 expression by short-term hyperglycemia and hyperinsulinemia that, in turn, could affect autonomic nervous system activity.
Alquier
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T
UMR CNRS 5018, Université Paul Sabatier, IFR 31, CHU Rangueil, Bât. L1, R-de-Ch. Bas, 1, avenue Jean Poulhès, 31403 Toulouse cedex, France.
Leloup
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C
Arnaud
E
E
Magnan
C
C
Pénicaud
L
L
D000818:Animals; D001111:Arcuate Nucleus of Hypothalamus / Q000166:cytology / Q000378:metabolism; D001786:Blood Glucose / Q000187:drug effects; D002531:Cerebellum / Q000166:cytology / Q000378:metabolism; D005260:Female; D005917:Globus Pallidus / Q000166:cytology / Q000378:metabolism; D005947:Glucose / Q000378:metabolism* / Q000494:pharmacology; D051275:Glucose Transporter Type 4; D006943:Hyperglycemia / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D006946:Hyperinsulinism / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D007026:Hypothalamic Area, Lateral / Q000166:cytology / Q000378:metabolism; D007031:Hypothalamus / Q000166:cytology / Q000378:metabolism*; D009004:Monosaccharide Transport Proteins / Q000235:genetics*; D009124:Muscle Proteins; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction
D001786:Blood Glucose; D051275:Glucose Transporter Type 4; D009004:Monosaccharide Transport Proteins; D009124:Muscle Proteins; D012333:RNA, Messenger; C495694:Slc2a4 protein, rat; D005947:Glucose