Cutting edge: the mouse NK cell-associated antigen recognized by DX5 monoclonal antibody is CD49b (alpha 2 integrin, very late antigen-2).
DX5 mAb is a useful reagent because it stains NK cells from all mouse strains examined. We have identified the molecule recognized by DX5 mAb by using a retrovirus-mediated expression cloning system. A 5-kb cDNA encoding a protein that is reactive with the DX5 mAb was isolated from a NK cell cDNA library, and this molecule was identical with CD49b (very late Ag-2, alpha(2) integrin). The DX5 mAb reacted with transfectants expressing CD49b, and binding of DX5 to the NK cells and CD49b transfectants was blocked in the presence of other anti-CD49b mAbs. When NK1.1(+) NK cells were cultured with IL-2, they progressively lost reactivity with DX5 mAb as a consequence of cellular proliferation. Cytotoxicity mediated by the DX5(+) NK cells was dramatically higher as compared with DX5(-) NK cells. Therefore, DX5 mAb recognizes CD49b and can be used to define functionally distinct subsets of NK cells.
Arase
H
H
Department of Microbiology and Immunology and Cancer Research Institute, University of California, San Francisco, CA 94143, USA.
Saito
T
T
Phillips
J H
JH
Lanier
L L
LL
D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism*; D000937:Antigen-Antibody Reactions; D015703:Antigens, CD / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology* / Q000502:physiology; D002460:Cell Line; D002478:Cells, Cultured; D002999:Clone Cells; D003001:Cloning, Molecular / Q000379:methods; D003601:Cytotoxicity Tests, Immunologic; D006801:Humans; D006825:Hybridomas; D039421:Integrin alpha2; D007376:Interleukin-2 / Q000494:pharmacology; D019169:Jurkat Cells; D007694:Killer Cells, Natural / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D051381:Rats; D011917:Rats, Inbred Lew
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D039421:Integrin alpha2; D007376:Interleukin-2