Neural retina of chick embryo in organ culture: effects of blockade of growth factors by suramin.
The neural retina is a highly organized organ whose final histoarchitecture depends on the presence of diverse growth factors and on their interactions with extracellular matrix components. However, the role of growth factors on retinal development is not fully understood. Suramin has been shown to produce diverse cellular effects via the simultaneous block of the action of several growth factors. We have therefore studied the effects of suramin on organotypic culture of chick embryo neural retina in order to gain further insights into the participation of growth factors in neural retinal development. Neural retina was incubated for 24 h with suramin at 50-200 microM and then processed to determine cell proliferation, nuclear morphology, and actin distribution. Suramin provoked extensive morphological changes revealed by a decrease in BrdU incorporation, alterations in cellular organization, and disruption of the outer limiting membrane, with the emergence of cellular elements through it. All of these effects were dose-dependent and markedly attenuated by the simultaneous presence of suramin and fibroblast growth factor 2 (FGF-2) in the culture medium. These findings indicate that suramin induces pleiotropic effects on the histoarchitecture of the chicken neural retina in organ culture and suggest that FGF-2 is one of the biological modulators involved in the maintenance of the structural organization of the chicken neural retina.
Cirillo
A
A
Departmento de Histología y Embriología, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay. acirillo@fmed.edu.uy
Chifflet
S
S
Villar
B
B
D000199:Actins / Q000378:metabolism; D000818:Animals; D017209:Apoptosis; D001973:Bromodeoxyuridine / Q000737:chemistry; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002467:Cell Nucleus / Q000187:drug effects / Q000648:ultrastructure; D002642:Chick Embryo; D003599:Cytoskeleton / Q000187:drug effects / Q000648:ultrastructure; D053938:DNA Fragmentation; D004305:Dose-Response Relationship, Drug; D016222:Fibroblast Growth Factor 2 / Q000037:antagonists & inhibitors*; D005453:Fluorescence; D006131:Growth Inhibitors / Q000494:pharmacology*; D007150:Immunohistochemistry; D009924:Organ Culture Techniques; D012160:Retina / Q000166:cytology / Q000187:drug effects / Q000196:embryology* / Q000648:ultrastructure; D013498:Suramin / Q000494:pharmacology*
D000199:Actins; D006131:Growth Inhibitors; D016222:Fibroblast Growth Factor 2; D013498:Suramin; D001973:Bromodeoxyuridine