Time profile of neuron specific enolase serum levels after experimental brain injury in rat.
The aim of this study was to investigate the time course of NSE serum levels after traumatic brain injury in rats. 65 male Wistar rats were subjected to severe cortical impact injury (100 PSI, 2 mm deformation). Blood samples were drawn directly after trauma, after 1 h, 6 h, 12 h, 24 h, and 48 h in the trauma group as well as in sham operated animals directly after craniotomy, after 6 h and after 48 h. NSE serum levels were estimated with a commercially available enzyme immuno assay (LIA-mat Sangtec). The control animals showed a NSE serum level of 8.82 micrograms/l (mean, n = 10). We demonstrated a time dependent release of NSE into the serum after trauma. The highest NSE serum values were detected six hours after trauma (31.5 micrograms/l, mean, n = 10). NSE serum level seems to reflect neuronal damage after cortical contusion in the rat in a time dependent manner.
Woertgen
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C
Department of Neurosurgery, University of Regensburg, Germany.
Rothoerl
R D
RD
Brawanski
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A
D000818:Animals; D001924:Brain Concussion / Q000201:enzymology*; D001929:Brain Edema / Q000201:enzymology*; D001930:Brain Injuries / Q000201:enzymology*; D002540:Cerebral Cortex / Q000201:enzymology / Q000293:injuries*; D008297:Male; D010751:Phosphopyruvate Hydratase / Q000097:blood*; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
D010751:Phosphopyruvate Hydratase