Mast cell mediation of muscle and pulmonary injury following hindlimb ischemia-reperfusion.
We have observed extensive mast cell degranulation in the reperfused hindlimb muscle of the mouse, accompanied by pathological changes within the muscle. As quantitated by the tissue:blood (125)I permeability ratio, both the hindlimbs and lungs exhibited a significant increment in permeability during hindlimb reperfusion. In lungs of the same mice, mast cell-derived chymase mMCP-1 coats alveolar macrophages, an event noted by us in acid-induced direct lung injury. Mast cells in the lung contain mMCP-1, whereas those in the muscle do not. Neither extensive muscle injury nor an increased pulmonary permeability index occurs in the mast cell-deficient W/W(v) mice. We conclude that the mast cell is a key mediator in both local ischemia-reperfusion injury (I-R) of muscle and consequent remote lung injury.
Mukundan
C
C
Department of Surgery, Brigham and Women's Hospital, and Harvard Medical School, Boston, Massachusetts, USA.
Gurish
M F
MF
Austen
K F
KF
Hechtman
H B
HB
Friend
D S
DS
D000818:Animals; D002199:Capillary Permeability; D015550:Cell Degranulation; D053818:Chymases; D006614:Hindlimb; D007150:Immunohistochemistry; D008168:Lung / Q000201:enzymology / Q000473:pathology*; D008297:Male; D008407:Mast Cells / Q000201:enzymology / Q000648:ultrastructure; D051379:Mice; D018482:Muscle, Skeletal / Q000201:enzymology / Q000473:pathology* / Q000648:ultrastructure; D009336:Necrosis; D015427:Reperfusion Injury / Q000473:pathology*; D022142:Secretory Vesicles / Q000201:enzymology; D012697:Serine Endopeptidases / Q000378:metabolism
D012697:Serine Endopeptidases; C083294:chymase 2; D053818:Chymases; C509229:Cma2 protein, mouse