title: Effect of serum depletion on centrosome overduplication and death of human pancreatic cancer cells after exposure to radiation.
abstract: The tumor microenvironment is one of the key factors affecting the cellular response to radiation; however, the influence of serum concentration on tumor radiosensitivity remains poorly understood. We recently discovered that gamma-irradiation of tumor cells causes centrosome overduplication, which may lead to lethal nuclear fragmentation through the establishment of multipolar mitotic spindles. In the present study, we investigated the effect of serum depletion on radiation-induced cell death in relation to the centrosome dynamics in human pancreatic cancer cells. Exposure of Capan-1 cells to gamma-irradiation resulted in a time-dependent increase in cells containing multiple centrosomes in association with the appearance of mitotic cell death. Treatment of irradiated cells with serum depletion drastically accelerated centrosome overduplication and the formation of multipolar spindles, resulting in increased nuclear fragmentation and cell death. Cell cycle analysis of irradiated cultures revealed that the reduced serum level increased the population of cells arrested in the G2/M phase, which might be responsible for the abnormal centrosome accumulation. These findings suggest that serum concentration can influence radiation-induced cell killing through modulating cell cycle progression and possibly centrosome overduplication.
authors.affiliation: Department of Surgery and Oncology, Graduate School of Medical Sciences, Kyushu University, 812-8582, Fukuoka, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Shono
authors.forename: N
authors.initials: N
authors.lastname: Sato
authors.forename: K
authors.initials: K
authors.lastname: Mizumoto
authors.forename: Y A
authors.initials: YA
authors.lastname: Minamishima
authors.forename: M
authors.initials: M
authors.lastname: Nakamura
authors.forename: N
authors.initials: N
authors.lastname: Maehara
authors.forename: T
authors.initials: T
authors.lastname: Urashima
authors.forename: M
authors.initials: M
authors.lastname: Saimura
authors.forename: L
authors.initials: L
authors.lastname: Qian
authors.forename: S
authors.initials: S
authors.lastname: Nishio
authors.forename: E
authors.initials: E
authors.lastname: Nagai
authors.forename: M
authors.initials: M
authors.lastname: Tanaka
mesh_terms: D016923:Cell Death / Q000528:radiation effects; D018385:Centrosome / Q000528:radiation effects*; D016895:Culture Media, Serum-Free; D006801:Humans; D008938:Mitosis / Q000528:radiation effects; D010190:Pancreatic Neoplasms / Q000235:genetics* / Q000473:pathology*; D014407:Tumor Cells, Cultured
chemical_list: D016895:Culture Media, Serum-Free