title: Effects of SCO-spondin thrombospondin type 1 repeats (TSR) in comparison to Reissner's fiber material on the differentiation of the B104 neuroblastoma cell line.
abstract: SCO-spondin is a newly identified protein that is strongly expressed in the subcommissural organ (SCO), an ependymal differentiation of the brain. When released into the cerebrospinal fluid at the entrance to the Sylvian aqueduct, the glycoproteins condense and form a thread-like structure, Reissner's fiber (RF). To analyze the role of SCO-spondin on neuronal development, we studied the effects induced by an oligopeptide derived from a thrombospondin type 1 repeat (TSR) of SCO-spondin on neuroblastoma B104 cells and compared them with the effects of soluble RF material containing complete SCO-spondin proteins. In low density cell culture, the TSR peptide first induced a notable flattening of cells accompanied by increased neurite outgrowth. Grouping of these differentiated B104 cells, which later formed dense aggregates, was then observed with increasing time in culture. Soluble RF material induced similar morphological changes and neurite-promoting effects on B104 cells, although the cells remained evenly distributed throughout the culture time and no aggregates were visible. In high-density cell culture, both TSR peptide and RF material induced prominent neurite outgrowth and subsequent rapid cell aggregation. Whereas soluble RF material inhibited cell proliferation, no respective effect was observed in the presence of the TSR peptide. A direct interaction of TSR peptide and soluble RF material with a B104 cell binding site was revealed by increased B104 cell metabolic activity by flow cytometry.
authors.affiliation: INSERM U 384 et Laboratoire de Biochimie Médicale, Faculté de Médecine, Clermont-Ferrand, France.
authors.forename: F
authors.initials: F
authors.lastname: El-Bitar
authors.forename: M
authors.initials: M
authors.lastname: Bamdad
authors.forename: B
authors.initials: B
authors.lastname: Dastugue
authors.forename: A
authors.initials: A
authors.lastname: Meiniel
mesh_terms: D020816:Amino Acid Motifs / Q000235:genetics; D000595:Amino Acid Sequence / Q000235:genetics; D000818:Animals; D015816:Cell Adhesion Molecules, Neuronal / Q000737:chemistry* / Q000494:pharmacology*; D002449:Cell Aggregation / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D048429:Cell Size / Q000187:drug effects; D017124:Conserved Sequence / Q000235:genetics; D005434:Flow Cytometry; D007700:Kinetics; D016501:Neurites / Q000187:drug effects / Q000648:ultrastructure; D009447:Neuroblastoma; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D009842:Oligopeptides / Q000494:pharmacology; D051381:Rats; D020449:Repetitive Sequences, Amino Acid; D019700:Thrombospondin 1 / Q000737:chemistry; D014407:Tumor Cells, Cultured
chemical_list: D015816:Cell Adhesion Molecules, Neuronal; D009842:Oligopeptides; C108402:SCO-spondin; D019700:Thrombospondin 1