title: YLR209c encodes Saccharomyces cerevisiae purine nucleoside phosphorylase.
abstract: The yeast YLR209c (PNP1) gene encodes a protein highly similar to purine nucleoside phosphorylases. This protein specifically metabolized inosine and guanosine. Disruption of PNP1 led to inosine and guanosine excretion in the medium, thus showing that PNP1 plays an important role in the metabolism of these purine nucleosides in vivo.
authors.affiliation: Institut de Biochimie et Génétique Cellulaires, CNRS UMR5095, F-33077 Bordeaux Cedex, France.
authors.forename: K
authors.initials: K
authors.lastname: Lecoq
authors.forename: I
authors.initials: I
authors.lastname: Belloc
authors.forename: C
authors.initials: C
authors.lastname: Desgranges
authors.forename: M
authors.initials: M
authors.lastname: Konrad
authors.forename: B
authors.initials: B
authors.lastname: Daignan-Fornier
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D003001:Cloning, Molecular; D004926:Escherichia coli; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics*; D006151:Guanosine / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D008969:Molecular Sequence Data; D011683:Purine-Nucleoside Phosphorylase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity
chemical_list: D005656:Fungal Proteins; D011994:Recombinant Proteins; D006151:Guanosine; D011683:Purine-Nucleoside Phosphorylase