A novel gene from Myxococcus xanthus that facilitates membrane translocation of an extracellular endoglucanase in Escherichia coli?
Expression in Escherichia coli of the Myxococcus xanthus gene celA, which encodes an extracellular endoglucanase, resulted in CelA being distributed between cytoplasm, periplasm and membrane. The presence of an adjacent open reading frame downstream from the full celA gene, or the absence of a putative lipoprotein signal sequence, confined CelA distribution to the periplasm and membrane, or to the cytoplasm and periplasm, respectively.
Bensmail
L
L
Laboratoire de microbiologie du froid, UFR des sciences et techniques, Université de Rouen, Mont-Saint-Aignan, France.
Monnier
C
C
Quillet
L
L
Guespin-Michel
J F
JF
Barray
S
S
D000595:Amino Acid Sequence; D002480:Cellulase / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D016941:Myxococcus xanthus / Q000737:chemistry / Q000201:enzymology / Q000235:genetics*; D016366:Open Reading Frames; D010957:Plasmids; D021382:Protein Sorting Signals
D008565:Membrane Proteins; D021382:Protein Sorting Signals; C074775:CelA endoglucanase; D002480:Cellulase