title: Purification, crystallization and identification by X-ray analysis of a prostate kallikrein from horse seminal plasma.
abstract: The purification, crystallization and identification by X-ray diffraction analysis of a horse kallikrein is reported. The protein was purified from horse seminal plasma. Crystals belong to space group C2 and the structure was solved by the MIRAS method, with two heavy-atom derivatives of mercury and platinum. X-ray diffraction data to 1.42 A resolution were collected at the ESRF synchrotron-radiation source.
authors.affiliation: Departamento de Química, Centro de Química Fina e Biotecnologia, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516 Monte de Caparica, Portugal.
authors.forename: A L
authors.initials: AL
authors.lastname: Carvalho
authors.forename: J M
authors.initials: JM
authors.lastname: Dias
authors.forename: L
authors.initials: L
authors.lastname: Sanz
authors.forename: A
authors.initials: A
authors.lastname: Romero
authors.forename: J J
authors.initials: JJ
authors.lastname: Calvete
authors.forename: M J
authors.initials: MJ
authors.lastname: Romão
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003460:Crystallization; D018360:Crystallography, X-Ray; D006736:Horses; D007610:Kallikreins / Q000737:chemistry* / Q000302:isolation & purification; D008297:Male; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011467:Prostate / Q000737:chemistry*; D011487:Protein Conformation; D012661:Semen / Q000737:chemistry*; D017386:Sequence Homology, Amino Acid
chemical_list: D007610:Kallikreins