Leptin receptor long-form signalling in a human liver cell line.
Expression of the long form of the leptin receptor was detected by reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blotting in the human liver cell line WRL68. Leptin (50-200 nM) significantly increased tyrosine phosphorylation of STAT cytoplasmic transcription factors STAT3 and STAT5b in a dose-dependent manner and produced a gel-shift with STAT3- and STAT5-specific oligonucleotides. WRL68 cells therefore provide the first human in vitro hepatocyte system in which to study leptin receptor-mediated signalling and to elucidate the role of leptin in liver.
Briscoe
C P
CP
Department of Vascular Biology, SmithKline Beecham Pharmaceuticals, New Frontiers Science Park, Harlow, Essex, UK. Celia_Briscoe@sbphrd.com
Hanif
S
S
Arch
J R
JR
Tadayyon
M
M
D002352:Carrier Proteins / Q000096:biosynthesis / Q000502:physiology*; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000378:metabolism; D006133:Growth Substances / Q000494:pharmacology; D006801:Humans; D020738:Leptin / Q000378:metabolism / Q000494:pharmacology; D008099:Liver / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D008894:Milk Proteins; D010766:Phosphorylation; D011956:Receptors, Cell Surface; D018121:Receptors, Cytokine / Q000096:biosynthesis / Q000502:physiology*; D054411:Receptors, Leptin; D050796:STAT3 Transcription Factor; D050799:STAT5 Transcription Factor; D015398:Signal Transduction / Q000502:physiology*; D015534:Trans-Activators / Q000378:metabolism; D014443:Tyrosine / Q000378:metabolism
D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D006133:Growth Substances; C515448:LEPR protein, human; D020738:Leptin; D008894:Milk Proteins; D011956:Receptors, Cell Surface; D018121:Receptors, Cytokine; D054411:Receptors, Leptin; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; D050799:STAT5 Transcription Factor; C494095:STAT5B protein, human; D015534:Trans-Activators; D014443:Tyrosine