'In vivo perfusion Turnbull's reaction' for Fe(II) histochemistry in non-anoxic/non-ischemic and anoxic/ischemic cat brains.
We developed a simple but ingenious histochemical method, 'in vivo perfusion-Turnbull's reaction', for the visualization of non-heme Fe(II) of the brain; in situ release of Fe(2+) ions was coupled with formation of insoluble reaction product (Turnbull's blue) by in vivo perfusion of acid ferricyanide through the abdominal (non-anoxic/non-ischemic brain) or ascending (anoxic/ischemic brain) aorta in the deeply anesthetized adult cats. Frozen sections of the brain were treated according to the method of Nyguen-Legros et al. [12] to intensify Turnbull's reaction. The method revealed that cytoplasmic Fe(III) was reduced to Fe(II) in oligodendroglias in anoxic/ischemic (for 20 min) brains, and that Fe(II) was concentrated in the neuronal and glial cell nuclei regardless of the presence or absence of blood supply impairment.
Yu
S
S
Department of Anatomy, Institute of Brain Science, Hirosaki University School of Medicine, Hirosaki 036-8562, Japan.
Iwatsuki
H
H
Ichinohe
N
N
Mori
F
F
Shoumura
K
K
D000818:Animals; D001921:Brain / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D002415:Cats; D002451:Cell Compartmentation / Q000502:physiology; D002467:Cell Nucleus / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D005260:Female; D005290:Ferric Compounds / Q000032:analysis; D005292:Ferricyanides / Q000494:pharmacology; D005295:Ferrocyanides / Q000032:analysis*; D005296:Ferrous Compounds / Q000032:analysis*; D020925:Hypoxia-Ischemia, Brain / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D008297:Male; D009474:Neurons / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism; D009836:Oligodendroglia / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism; D010477:Perfusion
D005290:Ferric Compounds; D005292:Ferricyanides; D005295:Ferrocyanides; D005296:Ferrous Compounds; C042827:potassium iron(III) hexacyanoferrate (II)