Molecular recognition in a reconstituted tumor cell membrane.
The design of an immunoliposome system for molecular recognition using reconstituted, hydrogel-supported bilayer lipid membranes (sb-BLMs) is described. By monitoring the electrical properties, two kinds of recognition are feasible: (i) the human bladder tumor cells, Ej and its antibody BDI-1, the lifetime of the reconstituted membrane is 42 min; and (ii) the human rectum tumor cells, LOVO, the life of the reconstructed membrane is more than 40 min, the same as conventional BLM. Further, the anticancer drug, Adriamycin (Anticancer Res., 20 (2000) 1391), was shown to be effective in such reconstituted systems, the life of which is less than 5 min. In these experiments, the active ingredients of the Ej and LOVO cells were determined on reconstituted sb-BLMs. The key point is that the component part being recognized on the BLM must be kept in its native state.
Hu
X
X
Department of Physical Pharmacy, School of Pharmaceutical Science, Peking University, Beijing 100083, People's Republic of China.
Wang
S
S
Zhang
Y
Y
Lu
X
X
Hou
X
X
Ottova
A
A
Tien
H T
HT
D000937:Antigen-Antibody Reactions / Q000502:physiology*; D000970:Antineoplastic Agents / Q000493:pharmacokinetics*; D002462:Cell Membrane / Q000378:metabolism; D004317:Doxorubicin / Q000493:pharmacokinetics*; D004563:Electrochemistry / Q000379:methods*; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000493:pharmacokinetics; D008051:Lipid Bilayers / Q000493:pharmacokinetics*; D008081:Liposomes; D014407:Tumor Cells, Cultured / Q000378:metabolism*
D000970:Antineoplastic Agents; D008051:Lipid Bilayers; D008081:Liposomes; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D004317:Doxorubicin