title: Genistein, a soy isoflavone, is a potent alpha-glucosidase inhibitor.
abstract: Genistein is an isoflavone that is known to be contained in soybean. It was proved that genistein plays a pivotal role in homeostasis in the human body. In the course of screening for useful alpha-glucosidase inhibitors, we isolated and identified genistein as a candidate for alpha-glucosidase inhibitor from fermentation broths of a Streptomyces sp. Genistein was shown to be a reversible, slow-binding, non-competitive inhibitor of yeast alpha-glucosidase with a K(i) value of 5.7x10(-8) M when the enzyme mixture was pretreated with genistein. These results show a possibility that genistein could be a useful tool for metabolic disorders.
authors.affiliation: Biophysical Chemisty Laboratory, The Institute of Physical and Chemical Research, RIKEN, Hyogo, Japan.
authors.forename: D S
authors.initials: DS
authors.lastname: Lee
authors.forename: S H
authors.initials: SH
authors.lastname: Lee
mesh_terms: D003956:Dialysis; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D005285:Fermentation; D019833:Genistein / Q000737:chemistry / Q000494:pharmacology*; D065089:Glycoside Hydrolase Inhibitors; D020128:Inhibitory Concentration 50; D007529:Isoflavones / Q000737:chemistry / Q000494:pharmacology*; D007700:Kinetics; D013025:Glycine max; D013302:Streptomyces / Q000737:chemistry; D015003:Yeasts / Q000201:enzymology; D000520:alpha-Glucosidases / Q000378:metabolism
chemical_list: D004791:Enzyme Inhibitors; D065089:Glycoside Hydrolase Inhibitors; D007529:Isoflavones; D019833:Genistein; D000520:alpha-Glucosidases