Structural characterization of phosphorylated chitosan and their applications as effective additives of calcium phosphate cements.
Chitosan was phosphorylated by P2O5 in methanesulfonic acid and the product as water-soluble phosphorylated chitosan (P-chitosans) was then characterized by phosphorus elemental analysis, IR and 31P-NMR spectroscopy. Two calcium phosphate cement (CPC) systems, i.e. (1) monocalcium phosphate monohydrate (MCPM) and calcium oxide (CaO) in 1 M phosphate buffer (pH = 7.4) and (2) dicalcium phosphate dihydrate (DCPD) and calcium hydroxide [Ca(OH)2] in 1 M Na2HPO4 solution, were chosen to improve their mechanical properties by the addition of water-soluble P-chitosans with various values for molecular weight, degree of deacetylation (DD) and degree of substitution (DS). The results show that the compressive strength (CS) and Young's modulus of both CPC formulations after setting were obviously increased and setting time was slightly prolonged by adding water-soluble P-chitosan to the liquid phases. When a suitable amount of P-chitosan was used, two improved CPC formulations were obtained with much better mechanical properties while the setting times were not longer than 15 min. The enhancement of compressive strength was due to high Ca2+-binding ability of phosphorylated chitosan, which could tightly bind the newly formed hydroxyapatite (HA) particles together by polymeric chains. It was noted that excessive addition of P-chitosan would lead to slow setting or no setting at all. The hardened CPC samples containing P-chitosan were characterized via X-ray diffraction spectra and scanning electron microscopy. Their leaching experiment was also carried out. The results indicated that P-chitosan-forced calcium phosphate cements have some good characteristics for clinical applications.
Wang
X
X
State Key Laboratory of Functional Polymer Materials for Adsorption and Separation, Institute of Polymer Chemistry, Nankai University, Tianjin, People's Republic of China.
Ma
J
J
Wang
Y
Y
He
B
B
D001843:Bone Cements / Q000737:chemistry*; D002130:Calcium Phosphates / Q000737:chemistry*; D002686:Chitin / Q000031:analogs & derivatives / Q000737:chemistry*; D048271:Chitosan; D009682:Magnetic Resonance Spectroscopy; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D015394:Molecular Structure; D008970:Molecular Weight; D010766:Phosphorylation; D012995:Solubility; D013055:Spectrophotometry, Infrared; D014961:X-Ray Diffraction
D001843:Bone Cements; D002130:Calcium Phosphates; C485828:alpha-tricalcium phosphate; C485830:tetracalcium phosphate; D002686:Chitin; C485838:calcium phosphate, monobasic, anhydrous; D048271:Chitosan; C020243:calcium phosphate; C485829:calcium phosphate, dibasic, anhydrous