title: Determination of the UV filters worldwide authorised in sunscreens by high-performance liquid chromatography. Use of cyclodextrins as mobile phase modifier.
abstract: Simultaneous determination of organic UV filters worldwide authorised in sunscreen formulations was performed by HPLC with UV spectrophotometric detection. The filters determined were: benzophenone-4, benzophenone-3, butyl methoxydibenzoylmethane, octyl dimethyl PABA, octyl methoxycinnamate, homosalate and octyl salicylate. A C18 stationary phase and an isocratic mobile phase of ethanol-water-acetic acid (70:29.5:0.5) containing 65.4 mM of hydroxypropyl-beta-cyclodextrin, were used with a flow-rate of 0.6 ml/min. UV measurements were carried out at 313 nm. The time required for the analysis was 20 min and the limits of detection were between 1.5 and 2.3 mg/l. The procedure proposed provides a green analytical method with a basic instrumental configuration, it is fast and accurate and does not involve highly toxic organic solvents.
authors.affiliation: Departamento de Química Analítica, Facultad de Química, Universitat de València, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Chisvert
authors.forename: M C
authors.initials: MC
authors.lastname: Pascual-Martí
authors.forename: A
authors.initials: A
authors.lastname: Salvador
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003505:Cyclodextrins / Q000737:chemistry*; D013473:Sunscreening Agents / Q000737:chemistry*; D014466:Ultraviolet Rays
chemical_list: D003505:Cyclodextrins; D013473:Sunscreening Agents