title: Extracellular polysaccharides of modified strains of Erwinia spp.
abstract: The structure of the extracellular polysaccharide (EPS) produced by Erwinia chrysanthemi strain A2148 has been determined using low pressure size-exclusion and anion-exchange chromatographies, high pH anion-exchange chromatography, glycosyl-linkage analysis, and 1D 1H NMR spectroscopy. The polysaccharide is structurally similar, if not identical, to the EPS produced by E. chrysanthemi strain A350. A streptomycin-resistant strain of E. chrysanthemi Ech6 (Ech6S(+)) has been generated and has an elevated production of EPS, as does a streptomycin-resistant strain (Ech9Sm6) of E. chrysanthemi Ech9. These modified E. chrysanthemi spp. have been ribotyped and found to be closely related to their parent strains.
authors.affiliation: Department of Biochemistry, College of Medicine, University of Iowa, 52242, Iowa City, IA, USA.
authors.forename: B Y
authors.initials: BY
authors.lastname: Yang
authors.forename: J M
authors.initials: JM
authors.lastname: Brand
authors.forename: J S
authors.initials: JS
authors.lastname: Gray
authors.forename: R
authors.initials: R
authors.lastname: Montgomery
mesh_terms: D002240:Carbohydrate Sequence; D002845:Chromatography / Q000379:methods; D016974:Dickeya chrysanthemi / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D004352:Drug Resistance, Microbial; D008969:Molecular Sequence Data; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D021521:Ribotyping; D013307:Streptomycin
chemical_list: D011135:Polysaccharides, Bacterial; D013307:Streptomycin