Separation of radioactive metabolites in cultured tea cells fed with [14C]phenylalanine using high-speed counter-current chromatography.
Separation of radioactive metabolites in cultured tea cells fed with [14C]phenylalanine was conducted using high-speed counter-current chromatography. Among seven components obtained our studies focused two metabolites, i.e. (-)-epicatechin and D,L-catechin. The specific radioactivity of (-)-epicatechin was 212.01 KBq/mg, amounting to 8.5% of the total radioactivity of ethyl acetate extract while that of D,L-catechin was 1.0006 MBq/mg or 5.4% of the total.
Du
Q
Q
Institute of Food and Biological Engineering, Hangzhou University of Commerce, China.
Cheng
H
H
Ito
Y
Y
D002250:Carbon Radioisotopes / Q000378:metabolism*; D002392:Catechin / Q000302:isolation & purification* / Q000378:metabolism; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D003377:Countercurrent Distribution / Q000379:methods*; D010649:Phenylalanine / Q000378:metabolism*; D013662:Tea / Q000166:cytology / Q000378:metabolism*
D002250:Carbon Radioisotopes; D013662:Tea; D010649:Phenylalanine; D002392:Catechin