A cryptic RRY(i) microsatellite from Atlantic salmon (Salmo salar): characterization and chromosomal location.
In this article we describe the isolation and characterization of a cryptic RRY(i) microsatellite from an Atlantic salmon genomic cosmid library. The chromosomal location of the microsatellite-containing cosmid was performed by fluorescent in situ hybridization (FISH) showing a single-locus signal located on an interstitial position of an acrocentric pair. The suitability of this type of microsatellite marker for population genetic analysis and for the development of a genetic map in this species is discussed. In addition, the usefulness of cosmid libraries for physical mapping of microsatellite markers and therefore for the integration of physical and genetic maps is pointed out.
Martinez
J L
JL
Departmento de Biologia Funcional, Facultad Medicina, Universidad de Oviedo, Julian Claveria s/n, 33006 Oviedo, Spain. jlmf@correo.uniovi.es
Moran
P
P
Garcia-Vazquez
E
E
D000483:Alleles; D000818:Animals; D002874:Chromosome Mapping; D020090:Chromosome Segregation; D015342:DNA Probes; D005260:Female; D015698:Genomic Library; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D016133:Polymerase Chain Reaction; D020317:Salmo salar / Q000235:genetics*; D018911:Trinucleotide Repeats / Q000235:genetics*
D015342:DNA Probes