Polysaccharide production by a reduced pigmentation mutant of Aureobasidium pullulans NYS-1.
OBJECTIVE
To isolate a reduced pigmentation mutant of Aureobasidium pullulans NYS-1 and characterize its cellular pigmentation plus its polysaccharide and biomass production relative to carbon source.


RESULTS
Cellular pigmentation, polysaccharide levels and biomass production by the isolated mutant NYSRP-1 were analysed relative to carbon source. Cellular pigmentation of the mutant was lower than its parent strain using either carbon source. The mutant elaborated higher polysaccharide levels on sucrose than on corn syrup. The pullulan content of the polysaccharide synthesized and biomass production by the mutant rose as the carbon source concentration was increased.


CONCLUSIONS
It is feasible to isolate a reduced pigmentation mutant from strain NYS-1 that exhibits elevated polysaccharide production using corn syrup as a carbon source.


CONCLUSIONS
The mutant provides an advantage for commercial pullulan production because of its reduced pigmentation and enhanced polysaccharide synthesis.
West
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TP
Olson Biochemistry Laboratories, Department of Chemistry and Biochemistry, South Dakota State University, Brookings, SD 57007, USA. Thomas_West@sdstate.edu
Strohfus
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B
D018533:Biomass; D002244:Carbon / Q000378:metabolism; D003470:Culture Media; D005936:Glucans / Q000096:biosynthesis* / Q000378:metabolism; D008543:Melanins / Q000235:genetics*; D003904:Mitosporic Fungi / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D009154:Mutation / Q000235:genetics*; D010858:Pigmentation / Q000235:genetics*; D013395:Sucrose / Q000378:metabolism; D003313:Zea mays
D003470:Culture Media; D005936:Glucans; D008543:Melanins; D013395:Sucrose; D002244:Carbon; C009109:pullulan