title: Increased generation of reactive oxygen species in isolated rat fatty liver during postischemic reoxygenation.
abstract: BACKGROUND
  Whether fatty infiltration of the liver influences the generation of reactive oxygen species (ROS) during reperfusion is unclear. Thus, this study aimed to compare the ROS formation that occurs during postanoxic reoxygenation in isolated normal and fatty livers.
  
  
  METHODS
  Isolated livers from fed Sprague-Dawley rats with normal or fatty livers induced by a choline-deficient diet were reperfused at 37 degrees C for 60 min with an oxygenated medium containing 10 microM of lucigenin after 1 hr of warm ischemia. Superoxide anion generation was assessed by the chemiluminescence (CLS) signal emitted from the organ surface. The hepatic content of malondialdehyde (MDA) and glutathione was determined at the end of reperfusion. Tissue injury was evaluated by the liver histology and the alanine aminotransferase (ALT) release in the perfusate.
  
  
  RESULTS
  CLS started rapidly with reoxygenation and it diffused to the whole organ in both groups. However, CLS emission was significantly higher in fatty liver (after 10 min: 812.425+/-39.898 vs. 294.525+/-21.068 photons/cm2/sec; P<0.01). A greater concentration of MDA was measured at the end of reoxygenation in fatty liver. Finally, the liver histology and the ALT release indicated a greater injury in steatotic than normal liver.
  
  
  CONCLUSIONS
  The CLS technique allows a direct visualization and comparison of ROS generation from the organ surface. Fatty infiltration increases ROS generation in the liver during postischemic reoxygenation, likely leading to the greater lipid peroxidation observed in these experiments. The increased oxidative stress may contribute to the reduced tolerance of steatotic livers to ischemia-reperfusion injury.
authors.affiliation: Dipartimento di Discipline Chirurgiche, Rianimatorie e dei Trapianti, University of Bologna, Policlinico Sant'Orsola, Via Massarenti 9, 40138 Bologna, Italy. nardo@orsola-malpighi.med.unibo.it
authors.forename: B
authors.initials: B
authors.lastname: Nardo
authors.forename: P
authors.initials: P
authors.lastname: Caraceni
authors.forename: P
authors.initials: P
authors.lastname: Pasini
authors.forename: M
authors.initials: M
authors.lastname: Domenicali
authors.forename: F
authors.initials: F
authors.lastname: Catena
authors.forename: G
authors.initials: G
authors.lastname: Cavallari
authors.forename: B
authors.initials: B
authors.lastname: Santoni
authors.forename: E
authors.initials: E
authors.lastname: Maiolini
authors.forename: I
authors.initials: I
authors.lastname: Grattagliano
authors.forename: G
authors.initials: G
authors.lastname: Vendemiale
authors.forename: F
authors.initials: F
authors.lastname: Trevisani
authors.forename: A
authors.initials: A
authors.lastname: Roda
authors.forename: M
authors.initials: M
authors.lastname: Bernardi
authors.forename: A
authors.initials: A
authors.lastname: Cavallari
mesh_terms: D000410:Alanine Transaminase / Q000378:metabolism; D000818:Animals; D005234:Fatty Liver / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D005978:Glutathione / Q000378:metabolism; D066298:In Vitro Techniques; D007511:Ischemia / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000473:pathology / Q000503:physiopathology; D008102:Liver Circulation; D008163:Luminescent Measurements; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D010100:Oxygen / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism*
chemical_list: D017382:Reactive Oxygen Species; D008315:Malondialdehyde; D000410:Alanine Transaminase; D005978:Glutathione; D010100:Oxygen