title: Evidence that replication fork components catalyze establishment of cohesion between sister chromatids.
abstract: Accurate chromosome segregation requires that replicated sister chromatids are held together until anaphase, when their "cohesion" is dissolved, and they are pulled to opposite spindle poles by microtubules. Establishment of new cohesion between sister chromatids in the next cell cycle is coincident with replication fork passage. Emerging evidence suggests that this temporal coupling is not just a coincident timing of independent events, but rather that the establishment of cohesion is likely to involve the active participation of replication-related activities. These include PCNA, a processivity clamp for some DNA polymerases, Trf4/Pol final sigma (formerly Trf4/Pol kappa), a novel and essential DNA polymerase, and a modified Replication Factor C clamp--loader complex. Here we describe recent advances in how cohesion establishment is linked to replication, highlight important unanswered questions in this new field, and describe a "polymerase switch" model for how cohesion establishment is coupled to replication fork progression. Building the bridges between newly synthesized sister chromatids appears to be a fundamental but previously unrecognized function of the eukaryotic replication machinery.
authors.affiliation: Department of Microbiology, Box 800734, Jordan Hall, 1300 Jefferson Park Avenue, University of Virginia, Charlottesville, VA 22908, USA.
authors.forename: D R
authors.initials: DR
authors.lastname: Carson
authors.forename: M F
authors.initials: MF
authors.lastname: Christman
mesh_terms: D001665:Binding Sites; D002384:Catalysis; D018797:Cell Cycle Proteins / Q000378:metabolism; D011508:Chondroitin Sulfate Proteoglycans; D002842:Chromatids; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism; D004258:DNA Polymerase III / Q000378:metabolism; D004261:DNA Replication; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D005656:Fungal Proteins / Q000378:metabolism; D006801:Humans; D009687:Nuclear Proteins; D016196:S Phase; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D000098550:Structural Maintenance of Chromosome Protein 1
chemical_list: D018797:Cell Cycle Proteins; D011508:Chondroitin Sulfate Proteoglycans; D002868:Chromosomal Proteins, Non-Histone; D005656:Fungal Proteins; D009687:Nuclear Proteins; C120372:SMC3 protein, S cerevisiae; C492318:SMC3 protein, human; D029701:Saccharomyces cerevisiae Proteins; D000098550:Structural Maintenance of Chromosome Protein 1; D004258:DNA Polymerase III; D004259:DNA-Directed DNA Polymerase; C487545:PAP2 protein, S cerevisiae; C118146:TENT4A protein, human