title: Cloning and biochemical characterization of a class A beta-lactamase from Prevotella intermedia.
abstract: The gene encoding a beta-lactamase of Prevotella intermedia was cloned and sequenced. This gene, called cfxA2, shared 98% identity with cfxA, the structural gene of a beta-lactamase previously described in Bacteroides vulgatus. The deduced protein sequence had a K272E substitution. CfxA2 had the characteristics of class A, group 2e beta-lactamases.
authors.affiliation: Laboratoire de Pathobiologie Orale, Faculté de Chirurgie Dentaire, 06357 Nice Cedex 4, France.
authors.forename: I
authors.initials: I
authors.lastname: Madinier
authors.forename: T
authors.initials: T
authors.lastname: Fosse
authors.forename: J
authors.initials: J
authors.lastname: Giudicelli
authors.forename: R
authors.initials: R
authors.lastname: Labia
mesh_terms: D000595:Amino Acid Sequence; D001439:Bacteroides / Q000201:enzymology; D003001:Cloning, Molecular; D017931:DNA Primers / Q000737:chemistry; D004269:DNA, Bacterial / Q000032:analysis; D006801:Humans; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D018713:Prevotella intermedia / Q000201:enzymology*; D017386:Sequence Homology, Amino Acid; D001618:beta-Lactamases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial; D001618:beta-Lactamases