Idiotype and antigen-specific T cell responses in mice on immunization with antigen, antibody, and anti-idiotypic antibody.
Idiotypic determinants of immunoglobulin molecules can evoke both CD4(+) and CD8(+) T responses and exist not only as the integral components of a bona fide antigen binding receptor but also as distinct molecular entities in the processed forms on the cell surface of B lymphocytes. The present work provides experimental evidence for the concept that regulation of memory B cell populations can be achieved through the presentation of idiotypic and anti-idiotypic determinants to helper and cytotoxic cell. The potential of B cells to present antigens to helper and cytotoxic T cells through class II and class I MHC suggests a mechanism by which both B and T cell homeostasis can be maintained. We provide evidence for the generation of idiotype- and antigen-specific Th and Tc cells upon immunization of syngenic mice with antigen or idiotypic antibody (Ab1) or anti-idiotypic antibody (Ab2). The selective activation and proliferation of the antigen-specific Th and Tc cells mediated by idiotypic stimulation observed in these experiments suggests a B-cell-driven mechanism for the maintenance of antigen-specific T cell memory in the absence of antigenic stimulation, under certain conditions.
Mitra-Kaushik
S
S
Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, 560012, India.
Shaila
M S
MS
Karande
A K
AK
Nayak
R
R
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D017951:Antigen Presentation; D001402:B-Lymphocytes / Q000276:immunology*; D005260:Female; D006023:Glycoproteins / Q000276:immunology; D006389:Hemagglutinins, Viral; D006825:Hybridomas / Q000276:immunology; D007111:Immunity, Cellular; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D007156:Immunologic Memory; D051379:Mice; D008807:Mice, Inbred BALB C; D018448:Models, Immunological; D013601:T-Lymphocytes / Q000276:immunology*; D014611:Vaccination; D014764:Viral Proteins / Q000276:immunology
D000888:Antibodies, Anti-Idiotypic; D006023:Glycoproteins; D006389:Hemagglutinins, Viral; D007130:Immunoglobulin Idiotypes; D014764:Viral Proteins; C079749:hemagglutinin protein, Rinderpest virus