[Comparative study of the morphology and antigenic properties of recombinant analogs of a Marburg virus nucleoprotein].
The full-length gene for Marburg virus (MV) nucleoprotein (NP) was cloned in prokaryotic pQE32 under the control of the T5 promoter and in eukaryotic pTM1 under the control of the promoter for T7 RNA polymerase. Recombinant NP was synthesized in Escherichia coli and in human kidney cell line 293 cotransfected with recombinant vaccinia virus vTF7-3 expressing T7 RNA polymerase. On evidence of electron microscopy with immune detection, recombinant NP formed tubules of two types in E. coli and of a single type in cell line 293. ELISA and immunoblotting with polyclonal and monoclonal antibodies revealed common antigenic determinants in recombinant NP and natural MV NP.
Kachko
A V
AV
a_kachko@chat.ru
Cheusova
T B
TB
Sorokin
A V
AV
Kazachinskaia
E I
EI
Cheshenko
I O
IO
Belanov
E F
EF
Bukreev
A A
AA
Ivanova
A V
AV
Razumov
I A
IA
Riabchikova
E I
EI
Netesov
S V
SV
D000911:Antibodies, Monoclonal / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D004274:DNA, Recombinant; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D029024:Marburgvirus / Q000378:metabolism*; D008854:Microscopy, Electron / Q000379:methods; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D016601:RNA-Binding Proteins; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D012261:Ribonucleoproteins; D014764:Viral Proteins
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D004274:DNA, Recombinant; D000939:Epitopes; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins; D016601:RNA-Binding Proteins; D011994:Recombinant Proteins; D012261:Ribonucleoproteins; D014764:Viral Proteins; C073094:nucleoprotein, Marburg virus