Effects of sodium hyaluronate on epithelial healing of the vesical mucosa and vesical fibrosis in rabbits with acetic acid induced cystitis.
OBJECTIVE
To evaluate the effect of sodium hyaluronate on epithelial healing of the vesical mucosa and vesical fibrosis, and clarify the effect of the sodium hyaluronate solution concentration, we administered sodium hyaluronate in the bladder of rabbits with acetic acid induced cystitis.


METHODS
Sodium hyaluronate at 3 concentrations (0.1%, 0.2% and 0.4%) was injected intravesically into rabbits with cystitis. Seven days after injection the effect of sodium hyaluronate was evaluated by bladder capacity measurement. Furthermore, the epithelial defective region of the mucosal membrane and bladder dry weight were determined and the condition of the epithelial membrane and extent of fibrosis were examined histologically.


RESULTS
In all sodium hyaluronate treated groups a significant improvement in bladder capacity was observed compared to controls. In addition, a significant reduction was noted in the area of the epithelial defective region in the groups treated with 0.2% or 0.4% sodium hyaluronate and a significant decrease was noted in bladder dry weight in the group treated with 0.4% sodium hyaluronate. Histological examination revealed accelerated epithelial healing of the vesical mucosa and inhibited vesical fibrosis in the group treated with 0.4% sodium hyaluronate.


CONCLUSIONS
Our findings suggest that sodium hyaluronate is effective for promoting epithelial healing of the vesical mucosa and inhibiting vesical fibrosis.
Takahashi
K
K
Central Research Laboratories, Seikagaku Corp., Higashiyamato, Tokyo, Japan.
Takeuchi
J
J
Takahashi
T
T
Miyauchi
S
S
Horie
K
K
Uchiyama
Y
Y
D019342:Acetic Acid; D000818:Animals; D003556:Cystitis / Q000139:chemically induced / Q000473:pathology*; D004848:Epithelium / Q000187:drug effects / Q000473:pathology; D005355:Fibrosis / Q000473:pathology; D006820:Hyaluronic Acid / Q000494:pharmacology*; D008297:Male; D009092:Mucous Membrane / Q000187:drug effects / Q000473:pathology; D009929:Organ Size; D011817:Rabbits; D014343:Trypan Blue; D001743:Urinary Bladder / Q000187:drug effects* / Q000473:pathology; D014945:Wound Healing / Q000187:drug effects
D006820:Hyaluronic Acid; D014343:Trypan Blue; D019342:Acetic Acid