The preparation and crystallization of Fab fragments of a family of mouse esterolytic catalytic antibodies and their complexes with a transition-state analogue.
The Fab fragments of a family of mouse esterolytic monoclonal antibodies MS6-12, MS6-126 and MS6-164 have been obtained by digestion of whole antibodies with papain, purified and crystallized in a range of different forms either alone or in complex with a transition-state analogue. The crystals diffract X-rays to resolutions between 2.1 and 1.2 A and are suitable for structural studies. The determination of these structures could be important in understanding the different catalytic power of each of these related catalytic antibodies.
Muranova
T A
TA
Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, Firth Court, Western Bank, Sheffield S10 2TN, England.
Ruzheinikov
S N
SN
Sedelnikova
S E
SE
Moir
A
A
Partridge
L J
LJ
Kakinuma
H
H
Takahashi
N
N
Shimazaki
K
K
Sun
J
J
Nishi
Y
Y
Rice
D W
DW
D000818:Animals; D017151:Antibodies, Catalytic / Q000737:chemistry* / Q000302:isolation & purification; D000911:Antibodies, Monoclonal / Q000737:chemistry; D003460:Crystallization; D018360:Crystallography, X-Ray; D007140:Immunoglobulin Fab Fragments / Q000737:chemistry* / Q000302:isolation & purification; D051379:Mice; D011487:Protein Conformation
D017151:Antibodies, Catalytic; D000911:Antibodies, Monoclonal; D007140:Immunoglobulin Fab Fragments