Determination of active component in silymarin by RP-LC and LC/MS.
Silybin, isosilybin, silydianin and silycristin in silymarin were separated and quantitatively determined by RP-HPLC. Two diastereoisomers of silybin and isosilybin were respectively separated by RP-HPLC and confirmed by LC/MS. Chromatographic condition consisted of column: Shim-pack VP-ODS (150 x 4.6mm i.d. 5 microm) and Pre-column (10 x 4.6 mm i.d. 5 microm); mobile phase: methanol and solvent mixture (water: dioxane=9:1) by gradient; flow rate: 1.5ml/min; column temp.: 40 degrees C; detector wavelength: 288 nm; The recovery of 99.66% for silycristin, 99.48% for silydianin, 100.0% for silybin and 98.72% for isosilybin was respectively obtained.
Ding
T
T
Hubei Institute for Drug Control, Wuhan 430064, People's Republic of China. dtm2000@sina.com
Tian
S
S
Zhang
Z
Z
Gu
D
D
Chen
Y
Y
Shi
Y
Y
Sun
Z
Z
D000975:Antioxidants / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D012838:Silymarin / Q000032:analysis*; D013237:Stereoisomerism
D000975:Antioxidants; D012838:Silymarin