[Sequence analysis of phosphoprotein genes of respiratory syncytial virus field strains isolated in China].
OBJECTIVE
To get information about phosphoprotein (P) genes of RSV field strains isolated in China.


METHODS
The complete nucleotide sequences of the P protein genes from six respiratory syncytial virus strains were determined with cDNA clones which had been amplified by RT-PCR and cloned into PTZ18R plasmid vector and compared with those of previously published counterparts of A2 and CH18537 strains.


RESULTS
The data indicated that there had high identities (>95%) at nucleotide and amino acid levels among the strains within the same subgroup; there were more divergence among strains (<85%) from different subgroups at nucleotide and amino acid levels of P genes, especially in the 3'-noncoding regions and the divergent domains. The analysis of hydrophobicity indicated that there had difference from 50 to 100 amino acid of P proteins between A and B subtypes.


CONCLUSIONS
The analysis of the P genes confirm that there existed variability among RSV field strains and strains A2 and CH18537. The data described above revealed the characteristics of the P genes of RSV field strains isolated in China.
Zhao
L
L
Beijing Municipal Laboratory of Infection and Immunity, Capital Institute of Pediatrics, Beijing 100020, China.
Wang
Z
Z
Geng
X
X
D001483:Base Sequence; D002681:China; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics; D012136:Respiratory Syncytial Viruses / Q000235:genetics* / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017421:Sequence Analysis; D017385:Sequence Homology; D014764:Viral Proteins / Q000235:genetics*
D010750:Phosphoproteins; D012367:RNA, Viral; D014764:Viral Proteins