Kinetics study of a selenium-containing ScFv catalytic antibody that mimics glutathione peroxidase.
The steady state kinetic study and some enzymic characterization of a selenium-containing scFv catalytic antibody (Se-scFv2F3) was carried out. A novel reaction formula of this abzyme-catalyzed reaction was proposed and a rate equation was gotten according to the formula. The constants in the equation were compared with Dalziel's parameters and the exact meanings of these constants were analyzed. The gotten kinetics parameters from the kinetics study of Se-scFv2F3 were analyzed and compared with that of native glutathione peroxidase.
Su
D
D
Key Laboratory of Molecular Enzymology and Engineering, Jilin University, Changchun 130023, People's Republic of China.
You
D
D
Ren
X
X
Luo
G
G
Mu
Y
Y
Yan
G
G
Xue
Y
Y
Shen
J
J
D017151:Antibodies, Catalytic / Q000737:chemistry*; D002384:Catalysis; D005978:Glutathione / Q000737:chemistry; D005979:Glutathione Peroxidase / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry; D007128:Immunoglobulin Fragments / Q000737:chemistry*; D007135:Immunoglobulin Variable Region / Q000737:chemistry; D007700:Kinetics; D010084:Oxidation-Reduction; D011506:Proteins / Q000737:chemistry*; D051140:Selenoproteins; D013379:Substrate Specificity
D017151:Antibodies, Catalytic; D007128:Immunoglobulin Fragments; D007135:Immunoglobulin Variable Region; D011506:Proteins; D051140:Selenoproteins; D006861:Hydrogen Peroxide; D005979:Glutathione Peroxidase; D005978:Glutathione