title: Comparison of SeroMP IgA with four other commercial assays for serodiagnosis of Mycoplasma pneumoniae pneumonia.
abstract: Acute phase serum samples from 23 patients with Mycoplasma pneumoniae pneumonia were examined for specific antibodies with microparticle agglutination (MAG)-assay, complement fixation (CF)-test, IgM-specific ELISA, and Immunocard-based IgM-EIA. A novel 3-h IgA-ELISA (SeroMP-IgA) was tested to assess its diagnostic value. For MAG, CF, IgM-ELISA, card-based IgM-EIA, and IgA-ELISA positive results were obtained in 87%, 87%, 91%, 87%, and 100%, respectively. Overall concordance was 78%. Specificity testing of 46 healthy blood donors revealed an optimal diagnostic cut-off range of 22-30 IgA-ELISA binding units (BU)/mL resulting in 91%-100% specificity and 100%-96% sensitivity for serologic diagnosis of acute M. pneumoniae infection.
authors.affiliation: Institute of Virology, University of Vienna, Division of Clinical Virology, General Hospital Vienna, Allgemeines Krankenhaus AKH-Wien, EBO/Leitstelle 4P, Waehringer Guertel 18-20, A-1090, Vienna, Austria. thomas.watkins-riedel@akh-wien.ac.at
authors.forename: T
authors.initials: T
authors.lastname: Watkins-Riedel
authors.forename: G
authors.initials: G
authors.lastname: Stanek
authors.forename: F
authors.initials: F
authors.lastname: Daxboeck
mesh_terms: D000293:Adolescent; D000328:Adult; D000907:Antibodies, Bacterial / Q000097:blood*; D002648:Child; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood*; D008875:Middle Aged; D009177:Mycoplasma pneumoniae / Q000276:immunology*; D011019:Pneumonia, Mycoplasma / Q000175:diagnosis*; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods; D013045:Species Specificity
chemical_list: D000907:Antibodies, Bacterial; D007070:Immunoglobulin A; D011933:Reagent Kits, Diagnostic