title: Retroviral splicing suppressor requires three nonconsensus uridines in a 5' splice site-like sequence.
abstract: Rous sarcoma virus RNA contains a negative regulator of splicing (NRS) element that aids in maintenance of unspliced RNA. The NRS binds U1 snRNA at a sequence that deviates from the 5' splice site consensus by substitution of U's for A's at three positions: -2, +3, and +4. All three of these U's are important for NRS-mediated splicing suppression. Substitution of a single nonconsensus C or G at any of these sites diminished NRS activity, whereas substitution of a single A generated a preferred 5' splice site within the NRS.
authors.affiliation: Department of Biology, Johns Hopkins University, Baltimore, Maryland 21218, USA.
authors.forename: R E
authors.initials: RE
authors.lastname: Paca
authors.forename: C S
authors.initials: CS
authors.lastname: Hibbert
authors.forename: C T
authors.initials: CT
authors.lastname: O'Sullivan
authors.forename: K L
authors.initials: KL
authors.lastname: Beemon
mesh_terms: D000818:Animals; D001358:Avian Sarcoma Viruses / Q000235:genetics*; D012326:RNA Splicing; D012367:RNA, Viral / Q000235:genetics*
chemical_list: D012367:RNA, Viral