Isolation and characterization of human foetal alpha-globulin (alpha 1F) from foetal and hepatoma sera.
The alpha 1F present in human foetal sera and the serum from a patient with hepatocellular carcinoma was isolated by immune precipitation using rabbit anti-alpha 1F. After labelling with 125I, some physicochemical properties of alpha 1F were investigated. The molecular weights of 125I-labelled alpha 1F isolated from foetal and hepatoma sera were 61,000 and 63,000, respectively. The protein was not dissociated in 6 M guanidine after complete reduction, showing that it contains a single peptide chain.
Adinolfi
A
A
Paediatric Research Unit, Guy's Hospital Medical School, London, S.E. 1., Great Britain.
Adinolfi
M
M
Cohen
S
S
D000510:Alpha-Globulins / Q000737:chemistry* / Q000302:isolation & purification; D000818:Animals; D000906:Antibodies; D006528:Carcinoma, Hepatocellular / Q000097:blood*; D005312:Fetal Blood / Q000737:chemistry; D005319:Fetal Hemoglobin / Q000737:chemistry* / Q000302:isolation & purification; D005333:Fetus; D006801:Humans; D007122:Immunoelectrophoresis; D008113:Liver Neoplasms / Q000097:blood*; D008970:Molecular Weight; D009439:Neuraminidase; D011817:Rabbits
D000510:Alpha-Globulins; D000906:Antibodies; D005319:Fetal Hemoglobin; D009439:Neuraminidase