[The leading role of mitochondrial depolarization in the mechanism of glutamate-induced disorder in Ca(2+)-homeostasis].
Digital fluorescence imaging techniques were employed to monitor changes in the cytoplasmic Ca2+ concentration and mitochondrial potential in fura-2 AM or rhodamine-123 loaded individual cerebellar granule cells during and following the Glu exposure. The data obtained suggests that the MD-induced blockade of the mitochondrial Ca2+ uptake and a reversal of the mitochondrial ATP-synthase play a critical role in the mechanism of the glutamate-induced disorder of neuronal Ca2+ homeostasis.
Khodorov
B I
BI
Institute of General Pathology and Pathophysiology, Russian Acad. Med. Sci., Baltiyskaya St., 8, Moscow 125315, Russia.
Storozhevykh
T P
TP
Surin
A M
AM
Iuriavichus
A I
AI
Sorokina
E G
EG
Borodin
A V
AV
Vinskaia
N P
NP
Khaspekov
L G
LG
Pinelis
V G
VG
D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors; D000255:Adenosine Triphosphate / Q000096:biosynthesis; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D004140:Dinitrophenols / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005456:Fluorescent Dyes; D016257:Fura-2; D018698:Glutamic Acid / Q000494:pharmacology / Q000502:physiology*; D006706:Homeostasis; D008564:Membrane Potentials; D008928:Mitochondria / Q000378:metabolism / Q000502:physiology*; D009474:Neurons / Q000378:metabolism* / Q000648:ultrastructure; D009840:Oligomycins / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D020112:Rhodamine 123; D012966:Sodium Cyanide / Q000494:pharmacology
D004140:Dinitrophenols; D004791:Enzyme Inhibitors; D005456:Fluorescent Dyes; D009840:Oligomycins; D020112:Rhodamine 123; D018698:Glutamic Acid; D000255:Adenosine Triphosphate; D000251:Adenosine Triphosphatases; D012966:Sodium Cyanide; D002118:Calcium; D016257:Fura-2