A simple HPLC method for the determination of the mycotoxins ochratoxin A and B in blood serum of swine.
This paper presents a simple method for the determination of ochratoxins A (OTA) and B (OTB) in pig blood serum. The method includes serum acidification (pH < 1.6) and precipitation of protein with 15% trichloroacetic acid, liquid partitioning with dichloromethane and fluorescence detection. The estimated detection limits were 0.1 ng OTA/ml and 0.2 ng OTB/ml. The mean recoveries from artificially contaminated samples (n = 6 replicates/mycotoxin) spiked at 0.3, 1 and 3ng OTA and OTB/ml, respectively, were 86.8% (s.d. = 8.4) for OTA and 90.0% (s.d. = 9.8) for OTB. Forty-nine Romanian pig blood serum samples (94% of 52 analysed) were found to be naturally contaminated with OTA in the range 0.1-13.4 ng/ml. No sample was found positive for OTB. The method is technically simple, specific, cost effective, suitable for large sample throughput and requires small amount of sample and reagents. It fulfils the criteria for a routine method and could be a suitable toolfor surveying OTA in pig herds and in slaughtered pigs.
Curtui
V G
VG
Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine of the Banat, Timisoara, Romania. vcurtui@yahoo.com
Gareis
M
M
D000818:Animals; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid / Q000379:methods / Q000592:standards; D009793:Ochratoxins / Q000097:blood*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013552:Swine / Q000097:blood*
D009793:Ochratoxins