Human retrovirus type 5 sequences in non-Hodgkin's lymphoma of T cell origin.
DNA of a recently described fifth exogenous retrovirus (HRV-5) has been found in blood samples from patients with autoimmune diseases and lymphoma. We analyzed HRV-5 sequence in DNA extracted from whole blood of 17 patients with T cell non-Hodgkin's lymphoma (NHL) and 186 patients with hematological malignancies other than NHL, using a sensitive PCR technique. While all samples of patients with hematological malignancies other than NHL were negative, 2 of the 17 patients with T cell NHL were HRV-5 DNA positive. Both HRV-5-positive patients had T cell NHL of high-grade malignancy (stage IV) and diffuse distribution of the lymphoma, including infiltration of bone marrow or lung and pleura. The difference in HRV-5 DNA detection frequency between NHL and control groups is significant (p value of 0.0004 judged by the Fisher exact test). These data, together with our previous finding of HRV-5 DNA in three B cell NHL cases, are compatible with an association between HRV-5 and NHL, of both T cell and B cell origin.
Kozireva
S
S
Department of Oncovirology, August Kirchenstein Institute of Microbiology and Virology, LV-1067 Riga, Latvia. skozireva@lycos.com
Lejniece
S
S
Blomberg
J
J
Murovska
M
M
D000595:Amino Acid Sequence; D001483:Base Sequence; D015331:Cohort Studies; D004279:DNA, Viral / Q000737:chemistry; D006801:Humans; D008228:Lymphoma, Non-Hodgkin / Q000097:blood / Q000821:virology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012190:Retroviridae / Q000235:genetics / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D013601:T-Lymphocytes / Q000276:immunology; D014764:Viral Proteins / Q000235:genetics*
D004279:DNA, Viral; D014764:Viral Proteins; C105040:pol protein, Rabbit endogenous retrovirus H