Characterization of a recombinant fowlpox virus expressing the native hexon of hemorrhagic enteritis virus.
The structure of the icosahedral adenovirus capsid is highly conserved among Adenoviridae. In its native form, the hexon is the major capsid protein. The nascent hexon requires the 100 kDa folding protein to fold into its native, trimeric form. The hexon and 100 kDa folding protein were co-expressed in a fowlpox virus (FPV) vector and in the recombinant FPVs (rFPVs) in which the hexon and 100 kDa folding protein genes are cloned head to tail, the native hexon could be detected with indirect immunofluorescence and immunoprecipitation using a native hexon monoclonal antibody. The FPV-@X100 construct, in which the 100kDa folding protein gene follows the hexon gene in a head to tail fashion, elicited the best humoral response in chickens. An attenuated HEV commercial vaccine elicited higher and longer lasting anti-HEV titers than FPV-@X100. Humoral immunity was also compared in turkeys inoculated with rFPVs expressing the hexon alone, the 100 kDa folding protein alone, or expressing both genes in different configurations. No anti-HEV humoral immune response was detected in turkeys inoculated with the rFPVs expressing the hexon alone or the 100 kDa folding protein alone.
Cardona
C J
CJ
Department of Pathology, Michigan State University, East Lansing, USA.
Nazerian
K
K
Reed
W M
WM
Silva
R F
RF
D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D001483:Base Sequence; D015139:Blotting, Southern; D015153:Blotting, Western; D002213:Capsid / Q000235:genetics*; D036022:Capsid Proteins; D002460:Cell Line; D017931:DNA Primers; D004797:Enzyme-Linked Immunosorbent Assay; D019084:Fluorescent Antibody Technique, Indirect; D005587:Fowlpox virus / Q000145:classification* / Q000235:genetics / Q000276:immunology; D018613:Microscopy, Confocal; D011233:Precipitin Tests; D011995:Recombination, Genetic
D000914:Antibodies, Viral; D036022:Capsid Proteins; D017931:DNA Primers; C018315:hexon capsid protein, Adenovirus