title: DNA ploidy analyses in 218 consecutive Pakistani breast cancer patients: does it add anything?
abstract: An analysis was made to evaluate the significance of DNA ploidy in the biology and prognosis of breast carcinoma. This was done by estimating the correlation of DNA ploidy with other established prognostic markers of breast cancer, namely tumor size, tumor grade, lymph node metastasis and S-phase fraction. From 1995 up to year 2000 ploidy analysis was performed on 218 consecutive cases of infiltrating breast carcinoma by flow cytometry using formalin fixed paraffin embedded material. From the laboratory record, data regarding other pathological variables was retrieved. No correlation could be found between DNA ploidy and tumor grade, nor could there be found a correlation with tumor size. For lymph node metastasis there was a significant difference between the proportion of aneuploids and diploids having metastasis in more than 4 lymph nodes. However, no significant difference was found in axillary lymph node positive and negative groups when number of positive lymph nodes was not taken into account. The mean value of S-phase fraction for the aneuploids and the diploids was also insignificantly different. In conclusion DNA ploidy alone did not add much to predict tumor behaviour in terms of known pathologic variables.
authors.affiliation: The Aga Khan University Medical Centre, Department of Pathology, Karachi, Pakistan.
authors.forename: E
authors.initials: E
authors.lastname: Khan
authors.forename: Z
authors.initials: Z
authors.lastname: Mapara
authors.forename: S
authors.initials: S
authors.lastname: Khan
authors.forename: N
authors.initials: N
authors.lastname: Arshad
authors.forename: T
authors.initials: T
authors.lastname: Siddiqui
authors.forename: S
authors.initials: S
authors.lastname: Pervez
mesh_terms: D000328:Adult; D000782:Aneuploidy; D001943:Breast Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D018270:Carcinoma, Ductal, Breast / Q000235:genetics / Q000401:mortality / Q000473:pathology; D004273:DNA, Neoplasm / Q000235:genetics*; D005260:Female; D006801:Humans; D008207:Lymphatic Metastasis; D010154:Pakistan / Q000453:epidemiology; D011003:Ploidies; D011379:Prognosis; D011446:Prospective Studies; D012307:Risk Factors; D016196:S Phase
chemical_list: D004273:DNA, Neoplasm