title: 4-Acetyl-12,13-epoxyl-9-trichothecene-3,15-diol isolated from the fruiting bodies of Isariajaponica Yasuda induces apoptosis of human leukemia cells (HL-60).
abstract: The fruiting bodies of Isaria fungi have been traditionally used in Korea to treat cancer. An apoptosis-inducing compound, 4-acetyl-12,13-epoxyl-9-trichothecene-3,15-diol, was isolated from the methanol extract of fruiting bodies of Isaria japonica Yasuda by bioassay-guided fractionation. The apoptosis of the human leukemia cells (HL-60) by the compound was accessed by propidium iodide-staining flow cytometric analysis, and apoptosis-inducing activity at IC50 concentration (10 nmol/l) was further confirmed by a nuclear morphological change, a ladder pattern of internucleosomal DNA fragmentation, and an activation of caspase-3.
authors.affiliation: Medicinal Resources Research Center of Wonkwang University, Iksan, Chonbuk, South Korea.
authors.forename: G S
authors.initials: GS
authors.lastname: Oh
authors.forename: K H
authors.initials: KH
authors.lastname: Hong
authors.forename: H
authors.initials: H
authors.lastname: Oh
authors.forename: H O
authors.initials: HO
authors.lastname: Pae
authors.forename: I K
authors.initials: IK
authors.lastname: Kim
authors.forename: N Y
authors.initials: NY
authors.lastname: Kim
authors.forename: T O
authors.initials: TO
authors.lastname: Kwon
authors.forename: M K
authors.initials: MK
authors.lastname: Shin
authors.forename: H T
authors.initials: HT
authors.lastname: Chung
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D004396:Coloring Agents; D053938:DNA Fragmentation / Q000187:drug effects; D004273:DNA, Neoplasm / Q000187:drug effects; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D018922:HL-60 Cells; D006801:Humans; D006999:Hypocreales / Q000737:chemistry*; D007211:Indoles; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D009504:Neutrophils / Q000187:drug effects; D013778:Tetrazolium Salts; D013844:Thiazoles; D014255:Trichothecenes / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: C440202:4-acetyl-12,13-epoxy-9-trichothecene-3,15-diol; D004396:Coloring Agents; D004273:DNA, Neoplasm; D007211:Indoles; D013778:Tetrazolium Salts; D013844:Thiazoles; D014255:Trichothecenes; C007293:DAPI; C022616:thiazolyl blue