LC determination of morphine and morphine glucuronides in human plasma by coulometric and UV detection.
A reversed-phase high-performance liquid chromatographic method with coulometric and UV detection has been developed for the simultaneous determination of morphine, morphine-3-glucuronide and morphine-6-glucuronide. The separation was carried out by using a Supelcosil LC-8 DB reversed-phase column and 0.1 M potassium dihydrogen phosphate (pH 2.5)--acetonitrile--methanol (94:5:1 v/v) containing 4 mM pentanesulfonic acid as the mobile phase. The compounds were determined simultaneously by coulometry for morphine and with UV detection for morphine-3-glucuronide and morphine-6-glucuronide. Morphine, morphine glucuronides and the internal standard were extracted from human plasma using Bond-Elut C18 (1 ml) solid-phase extraction cartridges. In the case of coulometric detection, the detection limit was 0.5 ng/ml for morphine; in the case of UV detection the detection limit was 10 ng/ml for morphine-3-glucuronide and for morphine-6-glucuronide, too.
Ary
K
K
Semmelweis University, Faculty of Health Sciences, Department of Ist Medicine, Clinical Pharmacological Laboratory, H-1389, P.O. Box 112, Budapest, Hungary.
Róna
K
K
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D003124:Colorimetry / Q000379:methods; D006801:Humans; D009020:Morphine / Q000097:blood* / Q000493:pharmacokinetics; D009022:Morphine Derivatives / Q000097:blood* / Q000493:pharmacokinetics; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013810:Therapeutic Equivalency
D009022:Morphine Derivatives; C035349:morphine-6-glucuronide; D009020:Morphine; C018108:morphine-3-glucuronide