title: (99m)Tc-HMPAO (Ceretec) is stored in and released from the granules of eosinophil granulocytes.
abstract: (99m)Tc-HMPAO (Ceretec) labelling of leucocytes is used clinically for the detection of inflammatory processes in the body. This study investigated the mechanisms by which (99m)Tc-HMPAO is taken up by eosinophils and neutrophils. Blood cells were labelled with (99m)Tc-HMPAO and the cells separated by means of their densities in Percoll gradients. For other purposes, eosinophils and neutrophils were purified by means of the MACS system and, after labelling these pure cellular preparations, the cells were ultrasonicated and the organelles separated on sucrose density gradients by means of ultracentrifugation. Organelles were characterized by their morphology on electron microscopy. Granulocytes were stimulated to secrete their granule constituents by means of exposure to complement-coated particles. ECP (eosinophil cationic protein) and MPO (myeloperoxidase) were measured using specific immunoassays. The uptake of (99m)Tc-HMPAO was 15--25-fold higher in eosinophils than in other leucocytes. (99m)Tc-HMPAO was predominantly stored in the secretory granules of eosinophils and released from the eosinophil, upon activation, together with ECP. A second storage compartment was a very light density organelle of unknown nature. These results indicated that, among leucocytes, (99m)Tc-HMPAO is preferentially taken up by eosinophils and stored in the secretory granules, which has to be taken into consideration when evaluating images based on this technique. Our findings suggest that (99m)Tc-HMPAO (Ceretec) may be used as a tool to follow eosinophil turnover and activity in disease.
authors.affiliation: Department of Medical Sciences, Clinical Chemistry, University of Uppsala, Uppsala, Sweden.
authors.forename: L
authors.initials: L
authors.lastname: Moberg
authors.forename: M
authors.initials: M
authors.lastname: Karawajczyk
authors.forename: P
authors.initials: P
authors.lastname: Venge
mesh_terms: D001798:Blood Proteins / Q000378:metabolism; D047091:Eosinophil Granule Proteins; D004804:Eosinophils / Q000378:metabolism*; D006801:Humans; D007249:Inflammation / Q000175:diagnosis; D007553:Isotope Labeling; D009504:Neutrophils / Q000378:metabolism; D015388:Organelles / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism; D012260:Ribonucleases; D022142:Secretory Vesicles / Q000378:metabolism*; D019690:Technetium Tc 99m Exametazime / Q000378:metabolism*; D013997:Time Factors
chemical_list: D001798:Blood Proteins; D047091:Eosinophil Granule Proteins; D019690:Technetium Tc 99m Exametazime; D009195:Peroxidase; D012260:Ribonucleases