title: B-cell-mediated lysis of cells infected with the neurotropic JHM strain of mouse hepatitis virus.
abstract: Cells expressing the spike (S) glycoprotein of the neurotropic JHM strain (JHMV) of mouse hepatitis virus (MHV) are susceptible to lysis by B cells derived from naïve mice, including B cells from perforin-deficient mice. Cytolysis requires interaction of the virus receptor and the viral S glycoprotein, is independent of other viral-induced components, and is not a unique property of B cells. Neutralizing anti-S-protein monoclonal antibodies (mAb) and a mAb specific for the viral receptor inhibit lysis. However, cells infected with an MHV strain unable to induce cell-cell fusion are resistant to lysis and lysis of JHMV-infected cells is inhibited by an anti-S-protein nonneutralizing mAb which prevents S-protein-mediated cell fusion. These data suggest that B cells may function as antibody-independent innate immune response during JHMV infection in vivo.
authors.affiliation: Department of Molecular Microbiology and Immunology, University of Southern California, Los Angeles, California 90033, USA.
authors.forename: S
authors.initials: S
authors.lastname: Morales
authors.forename: B
authors.initials: B
authors.lastname: Parra
authors.forename: C
authors.initials: C
authors.lastname: Ramakrishna
authors.forename: D M
authors.initials: DM
authors.lastname: Blau
authors.forename: S A
authors.initials: SA
authors.lastname: Stohlman
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D002450:Cell Communication / Q000276:immunology; D018352:Coronavirus Infections / Q000276:immunology* / Q000821:virology; D003602:Cytotoxicity, Immunologic; D007113:Immunity, Innate; D051379:Mice; D006517:Murine hepatitis virus / Q000276:immunology*; D009420:Nervous System / Q000821:virology