Overexpression of mitochondrial manganese superoxide dismutase protects against radiation-induced cell death in the human hepatocellular carcinoma cell line HLE.
We investigated the potential role of mitochondrial manganese superoxide dismutase (Mn-SOD) in protective activity against irradiation by analyzing cell viability by a colony formation assay and by detecting apoptosis in stably human Mn-SOD gene-transfected HLE, a hepatocellular carcinoma cell line. We found that overexpression of Mn-SOD reduced the levels of reactive oxygen species in the mitochondria and intracellular phospholipid peroxidation product (4-hydroxy-2-nonenal) and prevented cell death. The production of intracellular nitric oxide after irradiation was not changed by Mn-SOD overexpression. The results suggested that Mn-SOD might play an important role in protecting cells against radiation-induced cell death by controlling the generation of mitochondrial reactive oxygen species and intracellular lipid peroxidation.
Motoori
S
S
Research Center of Charged Particle Therapy, National Institute of Radiological Sciences, Chiba 263-8555, Japan.
Majima
H J
HJ
Ebara
M
M
Kato
H
H
Hirai
F
F
Kakinuma
S
S
Yamaguchi
C
C
Ozawa
T
T
Nagano
T
T
Tsujii
H
H
Saisho
H
H
D017209:Apoptosis / Q000235:genetics / Q000528:radiation effects; D006528:Carcinoma, Hepatocellular / Q000201:enzymology / Q000473:pathology*; D016923:Cell Death / Q000235:genetics / Q000528:radiation effects*; D002455:Cell Division / Q000235:genetics; D002470:Cell Survival / Q000235:genetics / Q000528:radiation effects; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D015227:Lipid Peroxidation / Q000528:radiation effects; D008928:Mitochondria / Q000201:enzymology* / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism / Q000528:radiation effects; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism*; D013997:Time Factors; D014162:Transfection; D014407:Tumor Cells, Cultured
D017382:Reactive Oxygen Species; D009569:Nitric Oxide; D013482:Superoxide Dismutase