Change in expression of GBP28/adiponectin in carbon tetrachloride-administrated mouse liver.
GBP28 (gelatin-binding protein of 28 kDa)/adiponectin is an adipocyte-producing plasma protein proposed to interact with the extracellular matrix. To examine the production of GBP28 in non-adipose tissues, we herein analyzed its expression and localization in mouse livers before and after CCl(4) treatment. In immunohistochemical analyses, the boundary of hepatocytes provided positive signals for GBP28 after 3-6 h and their cytoplasm was intensely stained after 18 h of CCl(4) treatment. Quantitative RT-PCR and in situ hybridization revealed that GBP28 mRNA expression was markedly elevated in CCl(4)-treated mouse livers. These results suggest that the circulating GBP28 binds the extracellular matrices of hepatocytes during the initial stage of CCl(4)-induced hepatic injury and the damaged hepatocytes themselves started to produce GBP28 thereafter. The induced expression of GBP28 was also observed in human hepatoma HepG2 cells after treatment with IL-6. Thus, GBP28 is also produced by the liver, where it undergoes tissue damage-induced transcriptional regulation.
Yoda-Murakami
M
M
Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan.
Taniguchi
M
M
Takahashi
K
K
Kawamata
S
S
Saito
K
K
Choi-Miura
N H
NH
Tomita
M
M
D052242:Adiponectin; D000818:Animals; D016375:Antisense Elements (Genetics); D002251:Carbon Tetrachloride / Q000633:toxicity*; D006528:Carcinoma, Hepatocellular; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D017403:In Situ Hybridization; D036341:Intercellular Signaling Peptides and Proteins; D015850:Interleukin-6 / Q000494:pharmacology; D007700:Kinetics; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D008113:Liver Neoplasms; D051379:Mice; D008807:Mice, Inbred BALB C; D011506:Proteins / Q000032:analysis / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic / Q000187:drug effects; D014407:Tumor Cells, Cultured
D052242:Adiponectin; D016375:Antisense Elements (Genetics); D036341:Intercellular Signaling Peptides and Proteins; D015850:Interleukin-6; D011506:Proteins; D012333:RNA, Messenger; D002251:Carbon Tetrachloride