Conformation of the glycosidic linkage in a disaccharide investigated by double-quantum solid-state NMR.
Double-quantum heteronuclear local field NMR is performed on a sample of a 13C2-labeled disaccharide, in which the two 13C spins are located on opposite sides of the glycosidic linkage. The evolution of the double-quantum coherences is found to be consistent with the solid-state conformation of the molecule, as previously determined by X-ray diffraction. The dependence of the double-quantum evolution on the glycosidic torsional angles is examined by using a graphical molecular manipulation program interfaced to a numerical spin simulation module.
Ravindranathan
S
S
Physical Chemistry Division, Stockholm University, Stockholm, S-10691, Sweden.
Karlsson
T
T
Lycknert
K
K
Widmalm
G
G
Levitt
M H
MH
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D003198:Computer Simulation; D004187:Disaccharides / Q000737:chemistry*; D006027:Glycosides / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data
D004187:Disaccharides; D006027:Glycosides