Elongation factor 1 beta/delta of Echinococcus granulosus and allergic manifestations in human cystic echinococcosis.
Allergic reactions, such as urticaria, itching and anaphylactic shock, often complicate the course of cystic echinococcosis (CE). To investigate the role of the IgE-immunoreactive recombinant Echinococcus granulosus elongation factor-1 beta/delta (EgEF-1 beta/delta) in the allergic disorders during CE we determined humoral and cell-mediated responses to this antigen in patients with CE grouped according to the clinical presence or absence of allergic reactions. Immunoblotting analysis showed that serum IgE-binding reactivity to EgEF-1 beta/delta differed significantly in patients with and without allergic reactions (38 of 42, 90% vs. 31 of 56, 56%; P < 10(-4)). EgEF-1 beta/delta induced a proliferative response in 14 of 19 (74%) patients' peripheral blood mononuclear cells (PBMC) irrespective of the allergic manifestations and skewed Th1/Th2 cytokine activation towards a preferentially Th2 polarization. Epitope mapping identified an immunodominant epitope of 18 residues with 78% identity and 89% similarity with an IgE-immunoreactive Strongyloides stercoralis antigen. Overall these findings suggest that EgEF-1 beta/delta is an allergenic molecule that may be a general marker of the intensity of CE immune response and that could lead to a deeper understanding of the specific antigen-induced mechanisms underlying allergic reactions in the human host.
Ortona
E
E
Department of Immunology, Istituto Superiore di Sanità, Rome, Italy.
Margutti
P
P
Vaccari
S
S
Riganò
R
R
Profumo
E
E
Buttari
B
B
Chersi
A
A
Teggi
A
A
Siracusano
A
A
D000328:Adult; D000368:Aged; D000595:Amino Acid Sequence; D000818:Animals; D004443:Echinococcosis / Q000276:immunology*; D004446:Echinococcus / Q000276:immunology*; D018604:Epitope Mapping; D005260:Female; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology*; D007073:Immunoglobulin E / Q000276:immunology; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D020648:Peptide Elongation Factor 1 / Q000276:immunology*
D020648:Peptide Elongation Factor 1; D007073:Immunoglobulin E