The RadB protein from Pyrococcus does not complement E. coli recA mutations in vivo.
A previous publication claimed that the radB gene called Pk-REC from Pyrococcus furiosus complemented an E. coli recA mutation. We found that a sequencing error had led to the test of a mutant form of Pk-REC. The wild-type radB gene from P. furiosus cloned in a similar expression vector to the mutant Pk-REC also appeared to complement an E. coli recA mutation. However, the cloned P. furiosus gdh (glutamate dehydrogenase) gene showed the same activity. We therefore concluded that overexpression of any protein can produce an artificial growth inhibition or stationary phase in recA mutant cells, which allows cells to recover from UV damage due to the action of repair systems that do not require RecA-like activity.
Inwood
W
W
Department of Molecular and Cell Biology, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
Kane
S
S
DiRuggiero
J
J
Robb
F
F
Clark
A J
AJ
D000595:Amino Acid Sequence; D019843:Archaeal Proteins; D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology*; D003001:Cloning, Molecular; D018388:Codon, Terminator / Q000235:genetics; D004249:DNA Damage; D004260:DNA Repair / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics / Q000528:radiation effects; D004268:DNA-Binding Proteins / Q000235:genetics / Q000502:physiology*; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development; D005816:Genetic Complementation Test; D005969:Glutamate Dehydrogenase / Q000235:genetics / Q000378:metabolism; D008954:Models, Biological; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D020027:Pyrococcus furiosus / Q000235:genetics*; D011836:Radiation Tolerance / Q000235:genetics; D011938:Rec A Recombinases / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000502:physiology; D014466:Ultraviolet Rays / Q000009:adverse effects
D019843:Archaeal Proteins; D001426:Bacterial Proteins; D018388:Codon, Terminator; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; C403141:RadB protein, Pyrococcus furiosus; D011993:Recombinant Fusion Proteins; D005969:Glutamate Dehydrogenase; D011938:Rec A Recombinases