title: Linear increase of diploidy in human sperm with age: a four-colour FISH study.
abstract: The aim of this study was to determine if donor age is associated with an increased incidence of diploidy and of disomy for the sex chromosomes and for chromosomes 6 and 21. We used simultaneous fluorescence in situ hybridisation (FISH) for chromosomes 6, 21, X and Y in sperm from 18 healthy donors, aged 24-74 years (mean 48.8 years). A total of 194 024 sperm were analysed, with a minimum of 10 000 sperm scored for each donor. Our results indicate a significant increase of the level of diploidy (P=0.002), and a marginal significance of total sex chromosome disomy (P=0.055) with age. No increase was observed for disomies XX, YY, XY, 21 or 6. The percentages of increase for disomy and for diploidy ranged from 0.3 to 17% for each 10-year period. Chromosomes 6 and 21 did not segregate preferentially with the X or Y chromosomes. Our findings show a linear trend association between age and diploidy in human males.
authors.affiliation: Departament de Biologia Cel.lular, Fisiologia i Immunologia, Facultat de Medicina, Universitat Autònoma de Barcelona, Bellaterra 08193, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Bosch
authors.forename: O
authors.initials: O
authors.lastname: Rajmil
authors.forename: O
authors.initials: O
authors.lastname: Martínez-Pasarell
authors.forename: J
authors.initials: J
authors.lastname: Egozcue
authors.forename: C
authors.initials: C
authors.lastname: Templado
mesh_terms: D000328:Adult; D000367:Age Factors; D000368:Aged; D000375:Aging; D000782:Aneuploidy; D002869:Chromosome Aberrations; D002891:Chromosomes, Human, Pair 21 / Q000235:genetics; D002896:Chromosomes, Human, Pair 6 / Q000235:genetics; D003627:Data Interpretation, Statistical; D004171:Diploidy; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D008297:Male; D008875:Middle Aged; D013094:Spermatozoa / Q000378:metabolism*; D014960:X Chromosome / Q000235:genetics; D014998:Y Chromosome / Q000235:genetics