Fastigiovestibular projections in the rat: retrograde tracing coupled with gammaamino-butyric acid and glutamate immunohistochemistry.
In this study, a double labeling technique using retrograde tracing with protein-gold complex (gold-HRP) in conjunction with a gammaamino-butyric acid (GABA) and glutamate immunohistochemical procedure was performed to identify GABA (GABA-IR) and glutamate (Glu-IR) immunoreactive neurons in the cerebellar fastigial nucleus (FN) that projects to the vestibular nuclei (VN). The results show that FN neurons projecting to the VN consist of both GABA-IR and Glu-IR neurons with a predominance of glutamatergic ones. Because GABAergic neurons in the cerebellar nuclei project to the inferior olive (IO), double retrograde labeling experiments were performed with injections of gold-HRP in the IO and of biotilynated dextran amine in the VN. This showed that the GABA-IR fastigiovestibular neurons project by axon collaterals to both the VN and the IO.
Diagne
M
M
Laboratoire de Neuroanatomie Fonctionnelle des Systèmes Sensorimoteurs, EA 3107, UP7, 2 Place Jussieu, case 7077, 75251 Cedex 05, Paris, France. diagne@ccr.jussieu.fr
Delfini
C
C
Angaut
P
P
Buisseret
P
P
Buisseret-Delmas
C
C
D000818:Animals; D001370:Axonal Transport / Q000187:drug effects / Q000502:physiology; D002452:Cell Count; D048429:Cell Size / Q000502:physiology; D002529:Cerebellar Nuclei / Q000166:cytology / Q000378:metabolism*; D018698:Glutamic Acid / Q000378:metabolism; D017612:Gold Compounds / Q000493:pharmacokinetics; D006735:Horseradish Peroxidase / Q000493:pharmacokinetics; D007150:Immunohistochemistry; D009434:Neural Pathways / Q000166:cytology / Q000378:metabolism*; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley / Q000033:anatomy & histology / Q000378:metabolism*; D014726:Vestibular Nuclei / Q000166:cytology / Q000378:metabolism*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
D017612:Gold Compounds; D018698:Glutamic Acid; D005680:gamma-Aminobutyric Acid; D006735:Horseradish Peroxidase