[Differences in the spatial structure of an envelope protein from tobacco mosaic virus and its mutant, detected by tritium planigraphy].
Mutant ts21-66 of the tobacco mosaic virus (TMV) differs from the wild-type TMV-U1 by two mutations (Ile-21-->Thr and Asp-66-->Gly) in the coat protein (CP) gene and in symptoms produced in infected N' plants. The CP structure in TMV-U1 and ts21-66 virions was probed by tritium planigraphy. Compared with the wild-type CP, labeling of the N-terminal region of mutant CP was half as high and suggested its greater shielding. A role of this CP region in virus interactions with the N' resistance system is discussed.
Lukashina
E V
EV
elena@radio.chem.msu.ru
Badun
G A
GA
Fedoseev
V M
VM
Fedorova
N V
NV
Ksenofontov
A L
AL
Baratova
L A
LA
Dobrov
E N
EN
D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009154:Mutation; D011487:Protein Conformation; D020349:Surface Plasmon Resonance; D014027:Tobacco Mosaic Virus / Q000737:chemistry* / Q000235:genetics; D014759:Viral Envelope Proteins / Q000737:chemistry*
D014759:Viral Envelope Proteins