Fatty and resin acid analysis in tall oil products via supercritical fluid extraction-supercritical fluid reaction using enzymatic catalysis.
Supercritical fluid extraction (SFE) is combined with supercritical fluid reaction (SFR) in an analytical mode to assess tall oil products for their fatty or resin acid content or both. The SFR consists of an inline enzymatically catalyzed reaction in which a lipase transesterifies specific lipids with methanol. The SFE-SFR sequence is conducted employing commercially available extractors using supported lipases in the extraction cell to form methyl esters. In this study, six different commercially available lipases are screened for activity. The SFE-SFR extracts are analyzed by capillary gas chromatography and supercritical fluid chromatography and then compared with tall oil products derivatized by conventional chemical derivatization techniques.
Taylor
S L
SL
Food Quality and Safety Research Unit, US Department of Agriculture, Peoria, IL 61604, USA. taylorsl@mail.ncaur.usda.gov
King
J W
JW