Pseudomonas brenneri sp. nov., a new species isolated from natural mineral waters.
The vernacular name 'fluorescent Pseudomonas group 97-391' was coined for a group of 11 strains isolated from two French natural mineral waters. All these strains were Gram-negative, rod-shaped and motile by means of a single polar flagellum. They produced fluorescent pigment (pyoverdin) on King B medium, catalase and cytochrome oxidase. They were not able to accumulate poly-beta-hydroxybutyrate. They were capable of respiratory but not fermentative metabolism. DNA-DNA hybridization results and DNA base composition analysis revealed that strains of the 'fluorescent Pseudomonas group 97-391' were members of a new species, for which the name Pseudomonas brenneri sp. nov. (type strain CIP 106646T) is proposed. The levels of DNA-DNA relatedness within this group ranged from 70 to 100% with DeltaTm below 1 degree C. The G+C content of the DNA of the type strain was 58 mol%. DNA relatedness with 72 strains representing well-known or partially characterized species of the genus Pseudomonas (sensu stricto) was below 48%. The complete 16S rRNA sequence of the type strain CIP 106646T was determined and compared with those of the type strains of Pseudomonas species. Finally, a phylogenetic tree was inferred from sequence analysis and demonstrated that the new species fell into the 'Pseudomonas fluorescens intrageneric cluster'. The clinical significance of P. brenneri is unknown.
Baïda
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Laboratoire de bactériologie-hygiène, Faculté de médecine Henri Warembourg. Lille, France.
Yazourh
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A
Singer
E
E
Izard
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D
D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D008900:Mineral Waters / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011549:Pseudomonas / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014871:Water Microbiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D008900:Mineral Waters; D012336:RNA, Ribosomal, 16S