Cell toxicity caused by products of the p(L) operon of bacteriophage lambda.
Induction of a lambda prophage causes the death of the host cell even in the absence of phage replication and lytic functions due to expression of functions from the lambda p(L) operon. We genetically modified the lambda prophage to determine which lambda p(L) operon functions were involved in cell killing. Viability assays and flow cytometry were used to monitor cell death and filamentation. The kil gene was shown to cause cell death and filamentation as described previously. Another killing activity was mapped within the p(L) operon to the gam gene. Inspection of the DNA sequence showed that there are two possible translation start points for both kil and gam. In both cases, the shorter of the two possible products could cause cell killing. The shorter products were also sufficient for the known filamentation and recombination activities of the respective Kil and Gam functions. The expression level of the p(L) operon is down-regulated by Cro repressor. In the absence of Cro, higher p(L) expression levels allow either Kil or Gam to be lethal or growth inhibitory, whereas at lowered expression in Cro-repressed conditions, only Kil is lethal. The filamentation function of Kil and recombination activity of Gam are unaffected at Cro-repressed levels of expression.
Sergueev
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National Cancer Institute at Frederick, Gene Regulation and Chromosome Biology Laboratory, Frederick, MD 21702-1201, USA.
Yu
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Austin
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Court
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D001426:Bacterial Proteins / Q000235:genetics; D010582:Bacteriophage lambda / Q000235:genetics*; D002455:Cell Division / Q000187:drug effects; D018387:Codon, Initiator / Q000235:genetics; D004268:DNA-Binding Proteins; D003673:Defective Viruses / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development / Q000821:virology; D029968:Escherichia coli Proteins; D017353:Gene Deletion; D015870:Gene Expression; D009876:Operon; D017354:Point Mutation; D011995:Recombination, Genetic; D012097:Repressor Proteins / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics*; D054334:Viral Regulatory and Accessory Proteins
D001426:Bacterial Proteins; D018387:Codon, Initiator; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D012097:Repressor Proteins; D014157:Transcription Factors; D014764:Viral Proteins; D054334:Viral Regulatory and Accessory Proteins; C054615:cIII protein, Bacteriophage lambda; C065410:gam protein, Coliphage; C069003:kil protein, E coli; C016649:phage repressor proteins