Constituents of compositae plants. 2. Triterpene diols, triols, and their 3-o-fatty acid esters from edible chrysanthemum flower extract and their anti-inflammatory effects.
The n-hexane soluble and the nonsaponifiable lipid fractions of the edible flower extract of chrysanthemum (Chrysanthemum morifolium) were investigated for triterpene diol and triol constituents. These triterpenes occur as the 3-O-fatty acid esters in the n-hexane soluble fraction from which 26 new and 6 known fatty acid esters were isolated and characterized. From the nonsaponifiable lipid fraction, 24 triterpene diols and triols were isolated, of which 3 were new compounds: (24S)-25-methoxycycloartane-3beta,24-diol (11), (24S)-25-methoxycycloartane-3beta,24,28-triol (22), and 22alpha-methoxyfaradiol (23). Faradiol (9) and heliantriol C (19), present in the nonsaponifiable lipid fraction and as the 3-O-palmitoyl esters in the n-hexane soluble fraction, were the most predominant triterpene diol and triol constituents. Fourteen triterpene diols and triols and 9 fatty acid esters were evaluated with respect to their anti-inflammatory activity against 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced inflammation in mice. All of the triterpenes examined showed marked inhibitory activity, with a 50% inhibitory dose (ID50) of 0.03-1.0 mg/ear, which was more inhibitive than quercetin (ID50 = 1.6 mg/ear), a known inhibitor of TPA-induced inflammation in mice.
Ukiya
M
M
College of Science and Technology, Nihon University, 1-8 Kanda Surugadai, Chiyoda-ku, Tokyo 101-8308, Japan.
Akihisa
T
T
Yasukawa
K
K
Kasahara
Y
Y
Kimura
Y
Y
Koike
K
K
Nikaido
T
T
Takido
M
M
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D005227:Fatty Acids; D007249:Inflammation / Q000139:chemically induced; D051379:Mice; D010936:Plant Extracts / Q000737:chemistry*; D010945:Plants, Edible / Q000737:chemistry*; D014315:Triterpenes / Q000032:analysis*
D000893:Anti-Inflammatory Agents; D005227:Fatty Acids; D010936:Plant Extracts; D014315:Triterpenes