Liquid chromatography of polyglycerol fatty esters and fatty ethers on porous graphitic carbon and octadecyl silica by using evaporative light scattering detection and mass spectrometry.
A liquid chromatographic method has been developed for the analysis of polyglycerol fatty esters and fatty ethers which are non-ionic surfactants. Two methods were compared using either octadecyl silica or porous graphitic carbon. The octadecyl silica system with a hydroorganic mobile phase enables to compare the hydrophobic behavior of the compounds. The porous graphitic carbon enables the separation of the diastereoisomers. Detection of these non-UV-absorbing compounds was achieved by evaporative light scattering detection. Identification of isomers was performed by mass spectrometry.
Cassel
S
S
ICOA, UMR CNRS 6005, Université d'Orléans, France. michel.lafosse@univ-orleans.fr
Chaimbault
P
P
Debaig
C
C
Benvegnu
T
T
Claude
S
S
Plusquellec
D
D
Rollin
P
P
Lafosse
M
M
D002853:Chromatography, Liquid / Q000379:methods*; D004952:Esters / Q000032:analysis*; D004987:Ethers / Q000032:analysis*; D005227:Fatty Acids / Q000737:chemistry*; D005990:Glycerol / Q000737:chemistry*; D006108:Graphite; D008027:Light; D013058:Mass Spectrometry; D011108:Polymers / Q000737:chemistry*; D012542:Scattering, Radiation; D012822:Silicon Dioxide
D004952:Esters; D004987:Ethers; D005227:Fatty Acids; D011108:Polymers; C043941:polyglycerol; D012822:Silicon Dioxide; D006108:Graphite; D005990:Glycerol