Cloning and characterization of 5'-upstream region of human phospholipase C-beta2 gene.
5'-upstream region of the phospholipase C-beta2 gene, 810 bp, was cloned and characterized. S1 nuclease mapping and primer extension analyses revealed that a single transcriptional start site locates at 284 nucleotides upstream from the beginning of translation. The 5-upstream region lacks both TATA motif and typical initiator sequence, but retains GC-rich segment. Two putative regulatory regions, a negative region (-636/-588) and a positive region (-98/ -13) were identified in the upstream region of PLC-beta2 gene. We suggest that the transcription of PLC-beta2 may be regulated by binding of regulatory proteins to the negative and/or positive regulatory regions located in the upstream of the gene.
Yun
E S
ES
Dept. Life Science, Pohang University of Science and Technology, Korea.
Lee
S J
SJ
Kim
M J
MJ
Ryu
S H
SH
Suh
P G
PG
D001483:Base Sequence; D002478:Cells, Cultured; D015500:Chloramphenicol O-Acetyltransferase / Q000378:metabolism; D003001:Cloning, Molecular; D017124:Conserved Sequence; D017353:Gene Deletion; D007527:Isoenzymes / Q000737:chemistry* / Q000235:genetics*; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D054799:Phospholipase C beta; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D015719:Single-Strand Specific DNA and RNA Endonucleases / Q000378:metabolism; D014158:Transcription, Genetic; D014162:Transfection; D010738:Type C Phospholipases / Q000737:chemistry* / Q000235:genetics*
D007527:Isoenzymes; D015500:Chloramphenicol O-Acetyltransferase; D015719:Single-Strand Specific DNA and RNA Endonucleases; D010738:Type C Phospholipases; C518036:PLCB2 protein, human; D054799:Phospholipase C beta