Horseradish peroxidase catalyzed degradation of industrially important dyes.
Horseradish peroxidase (HRP) is known to degrade certain recalcitrant organic compounds such as phenol and substituted phenols. Here, for the first time we have shown HRP to be effective in degrading and precipitating industrially important azo dyes. For Remazol blue, the enzyme activity was found to be far better at pH 2.5 than at neutral pH. In addition, Remazol blue acts as a strong competitive inhibitor of HRP at neutral pH. Horseradish peroxidase shows broad substrate specificity toward a variety of azo dyes. Kinetic constants (K(m)(app) and V(max)(app)) for two different dyes have been determined. In addition to providing a systematic analysis of the potential of HRP in degradation of dyes, this study opens up a new area on exploration of commercial dyes as inhibitors of enzymes. 2001 John Wiley & Sons, Inc.
Bhunia
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Department of Chemical Engineering, Indian Institute of Technology, Bombay, Powai, Mumbai 400 076, India.
Durani
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S
Wangikar
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D000880:Anthraquinones / Q000378:metabolism; D001391:Azo Compounds / Q000378:metabolism; D002384:Catalysis; D004396:Coloring Agents / Q000378:metabolism*; D004795:Enzyme Stability; D006735:Horseradish Peroxidase / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics
D000880:Anthraquinones; D001391:Azo Compounds; D004396:Coloring Agents; D006735:Horseradish Peroxidase; C024758:Remazol Brilliant Blue R