Respiration capacity of the fermenting bacterium Lactococcus lactis and its positive effects on growth and survival.
Oxygen is a major determinant of both survival and mortality of aerobic organisms. For the facultative anaerobe Lactococcus lactis, oxygen has negative effects on both growth and survival. We show here that oxygen can be beneficial to L. lactis if heme is present during aerated growth. The growth period is extended and long-term survival is markedly improved compared to results obtained under the usual fermentation conditions. We considered that improved growth and survival could be due to the capacity of L. lactis to undergo respiration. To test this idea, we confirmed that the metabolic behavior of lactococci in the presence of oxygen and hemin is consistent with respiration and is most pronounced late in growth. We then used a genetic approach to show the following. (i) The cydA gene, encoding cytochrome d oxidase, is required for respiration and plays a direct role in oxygen utilization. cydA expression is induced late in growth under respiration conditions. (ii) The hemZ gene, encoding ferrochelatase, which converts protoporphyrin IX to heme, is needed for respiration if the precursor, rather than the final heme product, is present in the medium. Surprisingly, survival improved by respiration is observed in a superoxide dismutase-deficient strain, a result which emphasizes the physiological differences between fermenting and respiring lactococci. These studies confirm respiratory metabolism in L. lactis and suggest that this organism may be better adapted to respiration than to traditional fermentative metabolism.
Duwat
P
P
Génétique Appliquée-URLGA, Institut National de la Recherche Agronomique, Domaine de Vilvert, 78352 Jouy en Josas, France.
Sourice
S
S
Cesselin
B
B
Lamberet
G
G
Vido
K
K
Gaudu
P
P
Le Loir
Y
Y
Violet
F
F
Loubière
P
P
Gruss
A
A
D000693:Anaerobiosis; D001426:Bacterial Proteins / Q000378:metabolism; D003470:Culture Media; D003573:Cytochrome b Group; D003580:Cytochromes / Q000378:metabolism; D045222:Electron Transport Chain Complex Proteins; D029968:Escherichia coli Proteins; D005285:Fermentation; D005294:Ferrochelatase / Q000378:metabolism; D006418:Heme / Q000378:metabolism*; D006859:Hydrogen / Q000378:metabolism; D013294:Lactococcus lactis / Q000254:growth & development* / Q000378:metabolism / Q000502:physiology; D010088:Oxidoreductases / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D013997:Time Factors
D001426:Bacterial Proteins; D003470:Culture Media; D003573:Cytochrome b Group; D003580:Cytochromes; D045222:Electron Transport Chain Complex Proteins; D029968:Escherichia coli Proteins; C121787:HemZ protein, Bacteria; D006418:Heme; D006859:Hydrogen; D010088:Oxidoreductases; C040077:cytochrome bd terminal oxidase complex, E coli; D005294:Ferrochelatase; D010100:Oxygen