[Expression of Trial in mammalian cell and its apoptosis activation in culture cells].
To develop a system for expression of Trail in mammalian cell. PCR and sequencing were used to clone and to confirm Trail gene. Then, we constructed the vector for expression of Trail in mammalian cells. HeLa cells, one of sensitive cell lines to Trail, were transfected with the vector DNA. After 48 h, flow cytometry was used to screen HeLa transfected by Trail. 19% of total cells happened to apoptosis. This study conforms that the production of Trail expressing in mammal cell can induce apoptosis and exhibit high bio-active. The results indicate the potential application of Trail to tumor immunotherapy.
Yao
Y C
YC
Department of Cell Biology, Second Military Medical University, Shanghai 200433, China.
Wang
X M
XM
Hu
Y P
YP
D017209:Apoptosis; D051017:Apoptosis Regulatory Proteins; D002478:Cells, Cultured; D006367:HeLa Cells; D006801:Humans; D008562:Membrane Glycoproteins / Q000235:genetics / Q000502:physiology*; D053221:TNF-Related Apoptosis-Inducing Ligand; D014162:Transfection; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000502:physiology*
D051017:Apoptosis Regulatory Proteins; D008562:Membrane Glycoproteins; D053221:TNF-Related Apoptosis-Inducing Ligand; C505653:TNFSF10 protein, human; D014409:Tumor Necrosis Factor-alpha