Seasonal variation in mussel Mytilus edulis digestive gland cytochrome P4501A- and 2E-immunoidentified protein levels and DNA strand breaks (Comet assay).
Mytilus edulis digestive gland microsomes were prepared from indigenous populations sampled from a clean reference site (Port Quin) and an urban-industrial contaminated site (Blackpool) in the UK. Samples were collected in March/April, May, August and December 1998. Western blot analysis was performed using polyclonal antibodies to fish CYP1A and rat CYP2E using partially purified M. edulis CYP as a positive control, to aid identification. CYP1A- and CYP2E-immunopositive protein levels showed different site-specific seasonal variation with higher levels of CYP2E determined in May (P < 0.05). At both sites, lower levels of CYP1A-immunopositive protein but not CYP2E-immunopositive protein were observed in the samples collected in December (P < 0.05). This correlated with lower levels of nuclear DNA damage (Comet assay expressed as per cent tail DNA) observed in December compared to August (P < 0.05).
Shaw
J P
JP
CCMS Plymouth Marine Laboratory, Citadel Hill, Plymouth, Devon PL1 2PB, UK.
Large
A T
AT
Chipman
J K
JK
Livingstone
D R
DR
Peters
L D
LD
D000818:Animals; D049872:Bivalvia / Q000201:enzymology*; D020552:Comet Assay; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism*; D019392:Cytochrome P-450 CYP2E1 / Q000378:metabolism*; D004064:Digestive System / Q000201:enzymology*; D008856:Microscopy, Fluorescence; D012621:Seasons
D019392:Cytochrome P-450 CYP2E1; D019363:Cytochrome P-450 CYP1A1