title: IL-7 differentially regulates cell cycle progression and HIV-1-based vector infection in neonatal and adult CD4+ T cells.
abstract: Differences in the immunological reactivity of umbilical cord (UC) and adult peripheral blood (APB) T cells are poorly understood. Here, we show that IL-7, a cytokine involved in lymphoid homeostasis, has distinct regulatory effects on APB and UC lymphocytes. Neither naive nor memory APB CD4(+) cells proliferated in response to IL-7, whereas naive UC CD4(+) lymphocytes underwent multiple divisions. Nevertheless, both naive and memory IL-7-treated APB T cells progressed into the G(1b) phase of the cell cycle, albeit at higher levels in the latter subset. The IL-7-treated memory CD4(+) lymphocyte population was significantly more susceptible to infection with an HIV-1-derived vector than dividing CD4(+) UC lymphocytes. However, activation through the T cell receptor rendered UC lymphocytes fully susceptible to HIV-1-based vector infection. These data unveil differences between UC and APB CD4(+) T cells with regard to IL-7-mediated cell cycle progression and HIV-1-based vector infectivity. This evidence indicates that IL-7 differentially regulates lymphoid homeostasis in adults and neonates.
authors.affiliation: Institut de Génétique Moléculaire de Montpellier, UMR 5535/IFR 22, F34293 Montpellier, France.
authors.forename: V
authors.initials: V
authors.lastname: Dardalhon
authors.forename: S
authors.initials: S
authors.lastname: Jaleco
authors.forename: S
authors.initials: S
authors.lastname: Kinet
authors.forename: B
authors.initials: B
authors.lastname: Herpers
authors.forename: M
authors.initials: M
authors.lastname: Steinberg
authors.forename: C
authors.initials: C
authors.lastname: Ferrand
authors.forename: D
authors.initials: D
authors.lastname: Froger
authors.forename: C
authors.initials: C
authors.lastname: Leveau
authors.forename: P
authors.initials: P
authors.lastname: Tiberghien
authors.forename: P
authors.initials: P
authors.lastname: Charneau
authors.forename: N
authors.initials: N
authors.lastname: Noraz
authors.forename: N
authors.initials: N
authors.lastname: Taylor
mesh_terms: D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D002453:Cell Cycle / Q000502:physiology*; D002455:Cell Division; D005434:Flow Cytometry; D005822:Genetic Vectors; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D015851:Interleukin-7 / Q000502:physiology*; D016176:T-Lymphocyte Subsets
chemical_list: D015851:Interleukin-7