[Identification of the protease-binding domain in the N-terminal region of the influenza A virus matrix protein M1].
A region responsible for protease binding by influenza virus (Flu) matrix protein M1 was identified. Trypsin binding was attributed to the N-terminal 9-kDa fragment obtained by hydrolyzing M1 with formic acid. The binding was inhibited by monoclonal antibodies (mAb) to the N-terminal moiety and by antiserum to region 21-45 of M1, whereas mAb to the middle and C-terminal regions had no effect. Thus, the protease-binding domain (PBD) was mapped to the N-terminal moiety of M1.
Timofeeva
T A
TA
Klenk
N D
ND
Zhirnov
O P
OP
D000595:Amino Acid Sequence; D001665:Binding Sites; D004591:Electrophoresis, Polyacrylamide Gel; D009980:Influenza A virus / Q000378:metabolism*; D008969:Molecular Sequence Data; D013379:Substrate Specificity; D014357:Trypsin / Q000378:metabolism*; D014361:Trypsin Inhibitors / Q000494:pharmacology; D014763:Viral Matrix Proteins / Q000737:chemistry / Q000378:metabolism*
C031540:M-protein, influenza virus; C488936:M1 protein, Influenza A virus; D014361:Trypsin Inhibitors; D014763:Viral Matrix Proteins; D014357:Trypsin