[Bovine alpha-sl-casein gene sequences direct expression of a variant of human tissue plasminogen activator in the milk of transgenic mice].
The fusion gene containing the promoter of casein gene, LAtPA minigene and 3' flanking sequences of casein gene was introduced into the fertilized eggs of mice by microinjection. Five positive transgenic mice were obtained. The concentration of LAtPA in the milk of one female transgenic mouse is 0.18 microgram/ml. This result showed that the LAtPA minigene could correctly express the bioactive LAtPA in the milk of the transgenic mouse under the control of the regulatory elements of the casein gene.
Tan
X H
XH
Molecnlar Genetics Key Laboratory, Institute of Biotechnology, Academy of Military Medical Sciences, Beijing 100071, China.
Cheng
X
X
Zhou
J
J
Chen
H X
HX
Lin
F Y
FY
Deng
J X
JX
Yang
X
X
Huang
P T
PT
D000818:Animals; D002364:Caseins / Q000235:genetics*; D002417:Cattle; D005260:Female; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic; D008845:Microinjections; D008892:Milk / Q000378:metabolism*; D009154:Mutation; D011401:Promoter Regions, Genetic; D010959:Tissue Plasminogen Activator / Q000235:genetics*
D002364:Caseins; D010959:Tissue Plasminogen Activator