title: Spin-label electron paramagnetic resonance studies on the interaction of avidin with dimyristoyl-phosphatidylglycerol membranes.
abstract: The interaction of avidin--a basic protein from hen egg-white--with dimyristoyl-phosphatidylglycerol membranes was investigated by spin-label electron paramagnetic resonance spectroscopy. Phosphatidylcholines, bearing the nitroxide spin label at different positions along the sn-2 acyl chain of the lipid were used to investigate the effect of protein binding on the lipid chain-melting phase transition and acyl chain dynamics. Binding of the protein at saturating levels results in abolition of the chain-melting phase transition of the lipid and accompanying perturbation of the lipid acyl chain mobility. In the fluid phase region, the outer hyperfine splitting increases for all phosphatidylcholine spin-label positional isomers, indicating that the chain mobility is decreased by binding avidin. However, there was no evidence for direct interaction of the protein with the lipid acyl chains, clearly indicating that the protein does not penetrate the hydrophobic interior of the membrane. Selectivity experiments with different spin-labelled lipid probes indicate that avidin exhibits a preference for negatively charged lipid species, although all spin-labelled lipid species indirectly sense the protein binding. The interaction with negatively charged lipids is relevant to the use of avidin in applications such as the ultrastructural localization of biotinylated lipids in histochemical studies.
authors.affiliation: School of Chemistry, University of Hyderabad, Hyderabad 500 046, India. mjssc@uohyd.ernet.in
authors.forename: M J
authors.initials: MJ
authors.lastname: Swamy
authors.forename: D
authors.initials: D
authors.lastname: Marsh
mesh_terms: D001360:Avidin / Q000737:chemistry*; D004578:Electron Spin Resonance Spectroscopy; D008567:Membranes, Artificial; D010715:Phosphatidylglycerols / Q000737:chemistry*; D013113:Spin Labels; D013696:Temperature
chemical_list: D008567:Membranes, Artificial; D010715:Phosphatidylglycerols; D013113:Spin Labels; D001360:Avidin; C002773:dimyristoylphosphatidylglycerol