Transgenic peanut plants obtained by particle bombardment via somatic embryogenesis regeneration system.
After pre-culture and treatment of osmosis, cotyledons of immature peanut (Arachis hypogaea L.) zygotic embryos were transformed via particle bombardment with a plasmid containing a chimeric hph gene conferring resistance to hygromycin and a chimeric intron-gus gene. Selection for hygromycin resistant calluses and somatic embryos was initiated at 10th d post-bombardment on medium containing 10-25 mg/L hygromycin. Under continuous selection, hygromycin resistant plantlets were regenerated from somatic embryos and were recovered from nearly 1.6% of the bombarded cotyledons. The presence and integration of foreign DNA in regenerated hygromycin resistant plants was confirmed by PCR (polymerase chain reaction) for the intron-gus gene and by Southern hybridization of the hph gene. GUS enzyme activity was detected in leaflets from transgenic plants but not from control, non-transformed plants. The production of transgenic plants are mainly based on a newly improved somatic embryogenesis regeneration system developed by us.
Deng
X Y
XY
Institute of Plant Physiology and Ecology, Chinese Academy Sciences, Shangha.
Wei
Z M
ZM
An
H L
HL
D000900:Anti-Bacterial Agents / Q000378:metabolism; D010367:Arachis / Q000235:genetics* / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods; D002678:Chimera / Q000235:genetics; D002934:Cinnamates; D018548:Cotyledon / Q000235:genetics; D004351:Drug Resistance / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000235:genetics*; D005818:Genetic Engineering / Q000379:methods*; D006921:Hygromycin B / Q000031:analogs & derivatives* / Q000378:metabolism; D009995:Osmosis / Q000502:physiology; D030821:Plants, Genetically Modified / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D012038:Regeneration / Q000235:genetics*; D012639:Seeds / Q000235:genetics*; D014170:Transformation, Genetic / Q000502:physiology*
D000900:Anti-Bacterial Agents; D002934:Cinnamates; D006921:Hygromycin B; C026273:hygromycin A