title: Comparison of tissue stages of Hepatozoon americanum in the dog using immunohistochemical and routine histologic methods.
abstract: American canine hepatozoonosis is caused by Hepatozoon americanum, a recently described species of apicomplexan protozoan parasite. An immunohistochemical procedure using a polyclonal antibody to sporozoites of H. americanum clearly identified asexual stages of H. americanum in canine striated muscle. The method also detects hepatozoa present in naturally infected coyotes and raccoons and reacts with certain other apicomplexans. Use of this immunohistochemical procedure confirms the canine intermediate host-parasite relationships that were presumptively established using conventional histopathologic methods.
authors.affiliation: Department of Pathobiology, College of Veterinary Medicine, Oklahoma State University, Stillwater 74078, USA.
authors.forename: R J
authors.initials: RJ
authors.lastname: Panciera
authors.forename: J S
authors.initials: JS
authors.lastname: Mathew
authors.forename: C A
authors.initials: CA
authors.lastname: Cummings
authors.forename: J C
authors.initials: JC
authors.lastname: Duffy
authors.forename: S A
authors.initials: SA
authors.lastname: Ewing
authors.forename: A A
authors.initials: AA
authors.lastname: Kocan
mesh_terms: D000818:Animals; D003048:Coccidiosis / Q000469:parasitology / Q000473:pathology / Q000662:veterinary*; D004283:Dog Diseases / Q000469:parasitology* / Q000473:pathology; D004285:Dogs; D016783:Eucoccidiida / Q000254:growth & development*; D006790:Host-Parasite Interactions; D007150:Immunohistochemistry / Q000662:veterinary; D018482:Muscle, Skeletal / Q000469:parasitology / Q000473:pathology; D011817:Rabbits