title: Protein adsorption equilibria and kinetics to a poly(vinyl alcohol)-based magnetic affinity support.
abstract: A poly(vinyl alcohol)-based magnetic gel entrapping Fe3O4 colloids has been prepared by an emulsification-crosslinking method. The gel was modified with Cibacron blue 3GA, and thus a magnetic affinity support was produced. The adsorption equilibrium studies showed that the adsorption isotherm of lysozyme was nearly rectangular, with a capacity of 254 mg/ml, while the adsorption isotherm of bovine serum albumin obeyed the Henry's law. Uptake kinetics of the two proteins was investigated and analyzed with a pore diffusion model and a homogeneous diffusion model. Experimental results showed that the magnetic affinity gel had magnetic responsiveness and favorable properties in protein adsorption, and was mechanically and chemically stable.
authors.affiliation: Department of Biochemical Engineering, Tianjin University, China.
authors.forename: B
authors.initials: B
authors.lastname: Xue
authors.forename: Y
authors.initials: Y
authors.lastname: Sun
mesh_terms: D000327:Adsorption; D008280:Magnetics; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*
chemical_list: D011506:Proteins; D011142:Polyvinyl Alcohol