title: Guanylin and uroguanylin in the parotid and submandibular glands: potential intrinsic regulators of electrolyte secretion in salivary glands.
abstract: The intestinal peptides guanylin and uroguanylin regulate the electrolyte/water transport in the gastrointestinal epithelium via activation of cystic fibrosis transmembrane conductance regulator (CFTR), the cystic fibrosis gene product. Because a major but incompletely understood function of the salivary glands is the CFTR-mediated secretion of an electrolyte-rich fluid, we investigated the rat and guinea pig parotid and submandibular glands for expression, cellular distribution, and subcellular localization of guanylin and uroguanylin. RT-PCR analyses with guanylin and uroguanylin-specific primers revealed that both peptides are highly expressed in the parotid and submandibular glands. At the translational level, western blotting analyses with peptide-specific guanylin and uroguanylin antibodies identified the expected 12.5-kDa immunoreactive peptides in these organs. At the cellular level, guanylin and uroguanylin were exclusively confined to epithelial cells of the intralobular and interlobular ducts. At the subcellular level, the immunoreactivities were localized by preembedding immunoelectron microscopy to small vesicles which were concentrated at the apical part of the secretory epithelial cells. The expression and cell-specific localization of guanylin and uroguanylin in the salivary glands indicate that these peptides may be specifically involved in the regulation of CFTR-mediated electrolyte/water secretion in the salivary gland ductal system.
authors.affiliation: Department of Molecular Cell Biology, Institute of Anatomy and Cell Biology, Philipps University, Robert-Koch-Strasse 6, 35033 Marburg, Germany. Cetiny@mailer.uni-marburg.de
authors.forename: H
authors.initials: H
authors.lastname: Kulaksiz
authors.forename: U
authors.initials: U
authors.lastname: Rausch
authors.forename: R
authors.initials: R
authors.lastname: Vaccaro
authors.forename: T G
authors.initials: TG
authors.lastname: Renda
authors.forename: Y
authors.initials: Y
authors.lastname: Cetin
mesh_terms: D000818:Animals; D005768:Gastrointestinal Hormones; D015870:Gene Expression; D006168:Guinea Pigs; D006801:Humans; D045265:Natriuretic Peptides; D010306:Parotid Gland / Q000378:metabolism; D010455:Peptides / Q000235:genetics / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D012469:Salivary Glands / Q000378:metabolism*; D013347:Subcellular Fractions; D013363:Submandibular Gland / Q000378:metabolism; D014882:Water-Electrolyte Balance / Q000502:physiology*
chemical_list: D005768:Gastrointestinal Hormones; D045265:Natriuretic Peptides; D010455:Peptides; C072685:guanylin; C084362:uroguanylin