title: Crystallization and preliminary X-ray study of endopolygalacturonase from the pathogenic fungus Stereum purpureum.
abstract: Crystals of endopolygalacturonase I from Stereum purpureum have been obtained by the vapour-diffusion method. Prior to crystallization work, endopolygalacturonase I was deglycosylated with endo-beta-N-acetylglucosaminidase H. The crystal diffracts to ultrahigh (0.96 A) resolution using synchrotron radiation and belongs to space group P1, with unit-cell parameters a = 37.26, b = 46.34, c = 52.05 A, alpha = 67.17, beta = 72.44, gamma = 68.90 degrees.
authors.affiliation: Kinetic Crystallography Research Team, RIKEN, Harima Institute at SPring-8, 1-1-1 Kouto, Mikazuki-cho, Sayo-gun, Hyogo 679-5148, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Shimizu
authors.forename: T
authors.initials: T
authors.lastname: Nakatsu
authors.forename: K
authors.initials: K
authors.lastname: Miyairi
authors.forename: T
authors.initials: T
authors.lastname: Okuno
authors.forename: H
authors.initials: H
authors.lastname: Kato
mesh_terms: D001487:Basidiomycota / Q000201:enzymology*; D003460:Crystallization; D018360:Crystallography, X-Ray; D011096:Polygalacturonase / Q000737:chemistry*; D011487:Protein Conformation
chemical_list: D011096:Polygalacturonase