Secretion of human proteins from yeast: stimulation by duplication of polyubiquitin and protein disulfide isomerase genes in Kluyveromyces lactis.
The efficiency of secreted production of mammalian proteins from yeasts remains unpredictably variable, depending on each protein. On the hypothesis that the control of protein conformation during protein translocation is the bottleneck in many cases, we examined the effects of an increased dosage of the genes coding for protein disulfide isomerase and of polyubiquitin on the secretion of two human proteins, serumalbumin and interleukin 1 beta. The yeast Kluyveromyces lactis was used as a production host. Duplication of either one of these genes had a strong stimulating effect on the production of the highly disulfide-bonded serumalbumin, but not of interleukin 1 beta.
Bao
W G
WG
Institut Curie, Section de Recherche, UMR 2027, Bâtiment 110, Centre Universitaire Paris XI, Orsay 91405, France.
Fukuhara
H
H
D001704:Biopolymers / Q000235:genetics; D015825:Chromosomes, Fungal / Q000235:genetics; D017077:Culture Media, Conditioned / Q000737:chemistry; D018628:Gene Dosage; D020440:Gene Duplication; D006801:Humans; D007375:Interleukin-1 / Q000235:genetics / Q000378:metabolism*; D007716:Kluyveromyces / Q000235:genetics / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics; D025821:Polyubiquitin; D019704:Protein Disulfide-Isomerases / Q000235:genetics; D011994:Recombinant Proteins / Q000378:metabolism; D012709:Serum Albumin / Q000235:genetics / Q000378:metabolism*; D014170:Transformation, Genetic; D014452:Ubiquitins / Q000235:genetics
D001704:Biopolymers; D017077:Culture Media, Conditioned; D007375:Interleukin-1; D011994:Recombinant Proteins; D012709:Serum Albumin; D014452:Ubiquitins; D025821:Polyubiquitin; D019704:Protein Disulfide-Isomerases