title: DNA adducts in hematopoietic tissues and blood of the mummichog (Fundulus heteroclitus) from a creosote-contaminated site in the Elizabeth River, Virginia.
abstract: Hydrophobic DNA adducts were examined in liver, anterior kidney, spleen, and blood of tumor-prone mummichog (Fundulus heterclitus) from the creosote-contaminated Atlantic Wood (AW) site (Elizabeth River, Virginia). DNA adducts eluted in a diagonal radioactive zone, characteristic of polycyclic aromatic hydrocarbon exposure, in all examined tissues of AW fish. Mummichog demonstrated significantly higher levels of DNA adducts in spleen (394 +/- 109 nmol adducts/mol nucleotides) than in liver (201 +/- 77 nmol adducts/mol nucleotides) or anterior kidney (211 +/- 68 nmol adducts/mol nucleotides; P = 0.036). The levels of DNA adducts in the pooled blood (pool of four) were 142 nmol adducts/mol nucleotides. DNA adducts were not detected in the liver, anterior kidney, spleen and blood of fish collected from the reference site (< 2 nmol adducts/mol nucleotides). The high levels of DNA adducts detected in tissues of AW mummichog may be linked to the increased cancer incidence and immunosuppression in this population.
authors.affiliation: Virginia Institute of Marine Science-School of Marine Science, College of William and Mary, Gloucester Point, VA 23062, USA.
authors.forename: W L
authors.initials: WL
authors.lastname: Rose
authors.forename: B L
authors.initials: BL
authors.lastname: French
authors.forename: W L
authors.initials: WL
authors.lastname: Reichert
authors.forename: M
authors.initials: M
authors.lastname: Faisal
mesh_terms: D000818:Animals; D001580:Benzopyrenes / Q000378:metabolism; D003407:Creosote / Q000633:toxicity*; D018736:DNA Adducts / Q000187:drug effects* / Q000378:metabolism; D006410:Hematopoiesis / Q000187:drug effects*; D007091:Image Processing, Computer-Assisted; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D007695:Killifishes / Q000097:blood*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D014768:Virginia; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D001580:Benzopyrenes; D018736:DNA Adducts; D014874:Water Pollutants, Chemical; C121255:anti-benzo(a)pyrene-dA adduct; D003407:Creosote