title: Evolving hardware as model of enzyme evolution.
abstract: Organism growth and survival is based on thousands of enzymes organized in networks. The motivation to understand how a large number of enzymes evolved so fast inside cells may be relevant to explaining the origin and maintenance of life on Earth. This paper presents electronic circuits called 'electronic enzymes' that model the catalytic function performed by biological enzymes. Electronic enzymes are the hardware realization of enzymes defined as molecular automata with a finite number of internal conformational states and a set of Boolean operators modelling the active groups of the active site. One of the main features of electronic enzymes is the possibility of evolution finding the proper active site by means of a genetic algorithm yielding a metabolic ring or k-cycle that bears a resemblance to Krebs (k=7) or Calvin (k=4) cycles present in organisms. The simulations are consistent with those results obtained in vitro evolving enzymes based on polymerase chain reaction (PCR) as well as with the general view that suggests the main role of recombination during enzyme evolution. The proposed methodology shows how molecular automata with evolvable features that model enzymes or other processing molecules provide an experimental framework for simulation of the principles governing metabolic pathways evolution and self-organization.
authors.affiliation: Department of Applied Mathematics, Faculty of Biological Sciences, Complutense University of Madrid, Madrid 28040, Spain. lahozraf@eucmax.sim.ucm.es
authors.forename: R
authors.initials: R
authors.lastname: Lahoz-Beltra
mesh_terms: D000465:Algorithms; D005075:Biological Evolution; D003198:Computer Simulation; D004798:Enzymes / Q000235:genetics*; D008956:Models, Chemical
chemical_list: D004798:Enzymes