Glomerular CD8+ cells predict progression of childhood IgA nephropathy.
The aim of this study was to evaluate whether the infiltrating T-lymphocyte can be a predictor in the disease progression of IgA nephropathy (IgAN). Twenty children with IgAN, followed for more than 5 years, were divided into progressive (n=5) and non-progressive groups (n=15). We assessed glomerular and interstitial infiltration of T-lymphocytes (CD4+ and CD8+ cells) and expression of alpha-smooth muscle actin (alpha-SMA) and transforming growth factor-beta (TGF-beta) using an indirect immunofluorescence method on the renal biopsies. We analyzed their relationship to the degree of proteinuria, histological changes, and prognosis. The number of CD8+ cells in glomeruli and in interstitium was higher in the progressive group than in the non-progressive group. The glomerular alpha-SMA staining was more intensive in the progressive group than in the non-progressive group. Urinary protein and the degree of histological changes were also higher in the progressive group than in the non-progressive group. Among these markers, the number of glomerular CD8+ cells was the most apparent difference between the two groups. In conclusion, these results indicate that the number of glomerular CD8+ cells is the most sensitive predictor of disease progression in childhood IgAN.
Watanabe
T
T
Department of Cell Biology, Institute of Nephrology, Faculty of Medicine, Niigata University, Japan.
Kawachi
H
H
Ikezumi
Y
Y
Yanagihara
T
T
Oda
Y
Y
Shimizu
F
F
D000199:Actins / Q000378:metabolism; D000293:Adolescent; D016827:CD8 Antigens / Q000378:metabolism*; D002648:Child; D018450:Disease Progression; D005260:Female; D005455:Fluorescent Antibody Technique; D005920:Glomerular Mesangium / Q000473:pathology; D005922:Glomerulonephritis, IGA / Q000473:pathology*; D006801:Humans; D007678:Kidney Glomerulus / Q000473:pathology*; D008297:Male; D009130:Muscle, Smooth / Q000378:metabolism; D011379:Prognosis; D011507:Proteinuria / Q000473:pathology; D012189:Retrospective Studies; D013601:T-Lymphocytes / Q000276:immunology / Q000473:pathology; D016212:Transforming Growth Factor beta / Q000378:metabolism
D000199:Actins; D016827:CD8 Antigens; D016212:Transforming Growth Factor beta