2,4,5-Trichlorophenoxyacetic acid inhibits apoptosis in PC12 cells.
2,4,5-Trichlorophenoxyacetic acid (2,4,5-T) is an endocrine disrupter that exerts cytotoxic effects on organisms. In this study, the influence of 2,4,5-T at low concentrations on apoptosis in PC 12 cells was investigated. Although no apoptotic features were observed in PC12 cells treated with 2,4,5-T, it inhibited the DNA fragmentation induced by serum deprivation. In addition, the cell viability of PC12 cells increased after treatment with 2,4,5-T. In conclusion, 2,4,5-T suppressed the apoptosis of the cultured cells. Since apoptosis is a morphological and biochemical description of a physiological mechanism of cell death that is commonly associated with programmed events necessary for development of individuals and organs, the inhibitory effect of 2,4,5-T on apoptosis might cause serious damage to cell homeostasis and differentiation.
Yamanoshita
O
O
Department of Environmental Medicine and Informatics, Graduate School of Environmental Earth Science, Hokkaido University, Sapporo, Japan.
Saito
T
T
Takahashi
K
K
Hosokawa
T
T
Okabe
M
M
Ito
K
K
Kurasaki
M
M
D015085:2,4,5-Trichlorophenoxyacetic Acid / Q000494:pharmacology*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D016895:Culture Media, Serum-Free / Q000494:pharmacology; D053938:DNA Fragmentation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004587:Electrophoresis, Agar Gel; D020287:In Situ Nick-End Labeling; D016627:Oxidopamine / Q000494:pharmacology; D016716:PC12 Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D051381:Rats
D016895:Culture Media, Serum-Free; D016627:Oxidopamine; D015085:2,4,5-Trichlorophenoxyacetic Acid