Comparative evaluation of Celsior solution versus Viaspan in a pig liver transplantation model.
BACKGROUND
In a pig liver transplantation model, we compared the effects of Celsior solution (CS), an extracellular preservation solution, with Viaspan (University of Wisconsin solution, UW) on graft function and animal survival.


METHODS
Pig livers were flushed with either CS or UW solution and cold-stored for 12 hr (group 1) or for 8 to 10 hr (group 2). Grafts were transplanted orthotopically. Intrahepatic reduced and oxidized glutathione and adenine nucleotides were evaluated 1 hr after reperfusion. Liver function of transplanted animals was monitored for up to 6 days by serum transaminases, total bilirubin, purine nucleoside phosphorylase, and prothrombin levels.


RESULTS
In group 1, all animals died within 24 hr after reperfusion regardless of the preservation solution used. In group 2, no significant difference was seen in survival between the CS (72%) and the UW (67%) groups 6 days after transplantation, and there were no statistically significant differences in the biochemical data. There were no differences in histological evaluation of the livers at the time of death or killing of the animals between the CS and UW groups.


CONCLUSIONS
Within the limits of this pilot study, CS is equivalent to UW in terms of graft function and animal survival.
Audet
M
M
Service de Chirurgie Générale et de Transplantation, Hôpital de Hautepierre, 67098 Strasbourg, France. Maxime.Audet@chru-strasbourg.fr
Alexandre
E
E
Mustun
A
A
David
P
P
Chenard-Neu
M P
MP
Tiollier
J
J
Jaeck
D
D
Cinqualbre
J
J
Wolf
P
P
Boudjema
K
K
D000241:Adenosine / Q000494:pharmacology*; D000493:Allopurinol / Q000494:pharmacology*; D000818:Animals; D004187:Disaccharides / Q000494:pharmacology*; D004573:Electrolytes / Q000494:pharmacology*; D005971:Glutamates / Q000494:pharmacology*; D005978:Glutathione / Q000494:pharmacology*; D006639:Histidine / Q000494:pharmacology*; D007328:Insulin / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects / Q000473:pathology / Q000503:physiopathology; D016031:Liver Transplantation; D008353:Mannitol / Q000494:pharmacology*; D019188:Organ Preservation Solutions / Q000494:pharmacology*; D011887:Raffinose / Q000494:pharmacology*; D016019:Survival Analysis; D013552:Swine; D016042:Transplantation, Heterotopic
C088453:Celsior; D004187:Disaccharides; D004573:Electrolytes; D005971:Glutamates; D007328:Insulin; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D008353:Mannitol; D006639:Histidine; D000493:Allopurinol; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose