title: Dialysis cells for controlled DNA: drug binding studies.
abstract: An efficient small volume accurate dialysis system has been designed, built and tested for proflavine binding to DNA based upon the side by side design used in the Franz diffusion cell. In a typical experiment 3 cm3 of DNA solution is added to one side and 3 cm3 of ligand to the other with a dialysis membrane between the two sides of 1 cm in diameter, thereby minimizing the area of dialysis membrane that the solutions are in contact with.
authors.affiliation: Department of Chemistry, University of Warwick, Coventry CV4 7AL, UK.
authors.forename: K J
authors.initials: KJ
authors.lastname: Sanders
authors.forename: H
authors.initials: H
authors.lastname: Wiles
authors.forename: A
authors.initials: A
authors.lastname: Rodger
mesh_terms: D000818:Animals; D000891:Anti-Infective Agents, Local / Q000378:metabolism*; D002417:Cattle; D004247:DNA / Q000378:metabolism*; D003956:Dialysis; D011370:Proflavine / Q000378:metabolism*; D011485:Protein Binding
chemical_list: D000891:Anti-Infective Agents, Local; D004247:DNA; D011370:Proflavine