An in vitro comparison of human flexor and extensor tendon cells.
This study examined the in vitro phenotype of cells cultured from both flexor and extensor tendons. Matrix proteins secreted by tendon cells were examined, together with their response to mechanical strain, using cells from the two types of tendon. Immunocytochemical staining using specific antibodies to matrix proteins demonstrated that flexor tendon cells synthesised only three of the five proteins stained for, whilst extensor tendon cells synthesised all five. Gel electrophoresis (used to separate mixtures of proteins) demonstrated that protein secretion was similar for both cell types. Both cell types showed similar patterns of synthesis for total collagen and total protein over time. No difference was found in the response to cyclical strain of cells from the two types of tendon; both cell proliferation and collagen synthesis were stimulated. The study demonstrated that cells obtained from two different types of tendon behave similarly when exposed to identical environmental conditions in vitro.
Evans
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University of Manchester, Manchester, UK. cevans@fs1.ho.man.ac.uk
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D002478:Cells, Cultured; D006801:Humans; D007150:Immunohistochemistry; D013314:Stress, Mechanical; D013710:Tendons / Q000166:cytology* / Q000502:physiology*