[Affinity chromatography of nucleoprotein complexes].
Affinity sorbent containing cellular membranes of the cerebral cortex was obtained by means of an inclusion into porous matrix of polyacrylonitrile. Sorption of model and natural nucleoprotein complexes on the obtained sorbent was studied by the method of affinity chromatography. Distribution coefficients were determined. Natural nucleoprotein complexes isolated from brain possess a greater selectivity of binding to the cellular membranes of the cerebral cortex than that from liver.
Riadnova
I Iu
IIu
St. Petersburg Institute of Bioregulation and Gerontology, North-Western Department of the Russian Academy of Medical Sciences, 197110, Russia, St. Peterburg.
Chernova
I A
IA
Shataeva
L K
LK
Khavinson
V Kh
VKh
D000180:Acrylic Resins; D000818:Animals; D002417:Cattle; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism*; D002846:Chromatography, Affinity; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D066298:In Vitro Techniques; D007328:Insulin / Q000737:chemistry; D036341:Intercellular Signaling Peptides and Proteins; D008099:Liver / Q000737:chemistry; D009698:Nucleoproteins / Q000737:chemistry / Q000378:metabolism*; D010455:Peptides / Q000737:chemistry; D011485:Protein Binding; D013154:Spleen / Q000737:chemistry
D000180:Acrylic Resins; D007328:Insulin; D036341:Intercellular Signaling Peptides and Proteins; D009698:Nucleoproteins; D010455:Peptides; C010504:polyacrylonitrile; C056590:cortexin; D004247:DNA