Effect of intrahepatic omental implantation on angiogenesis in rat liver with hepatic artery ligation.
Despite early surgical intervention, the results of treating hepatic artery thrombosis remain poor. The use of omental flaps is well documented for its angiogenic potential in promoting neovascularization in ischemic tissues. This experimental study evaluated the formation of new blood-vessels after omental implantation (OI) in rats after ligation of the hepatic artery. Wistar rats were used and divided into the following groups: I OI with HAL, II OI without HAL, III hepatic artery ligation (HAL). For angiography, measurements were made of liver tissue blood flow by the laser Doppler method and of hepatic artery flow by the colored microsphere method (CMS), and immunohistochemical study was done for vascular endothelial growth factor (VEGF). The rats were killed at 1, 3, 7 or 30 days after laparotomy. Relative arterial hepatic blood flow in the implanted lobe of group I, as determined by CMS, reached 50% of control values 7 days after surgery. Angiography and microscopic studies of the excised liver revealed distinct angiogenesis surrounding the omental implant in the liver for 7 days postoperatively. The formation of new blood-vessels after OI was not observed in livers without HAL. Omental implantation appears to be useful in preventing organ anoxia after hepatic artery thrombosis.
Ishikawa
M
M
Department of Surgery, Kochi National Hospital, Asakura-Nishimachi, Kochi City, Japan. masashi@kochi.hosp.go.jp
Fjii
M
M
Iuchi
M
M
Miyauchi
T
T
Tashiro
S
S
D000818:Animals; D001783:Blood Flow Velocity; D006499:Hepatic Artery / Q000502:physiology*; D007511:Ischemia / Q000503:physiopathology; D017078:Laser-Doppler Flowmetry; D008099:Liver / Q000098:blood supply*; D008297:Male; D018919:Neovascularization, Physiologic / Q000502:physiology*; D009852:Omentum / Q000637:transplantation*; D051381:Rats; D017208:Rats, Wistar; D012039:Regional Blood Flow; D013927:Thrombosis / Q000503:physiopathology*; D013997:Time Factors