title: Effects of the garlic compound diallyl disulfide on the metabolism, adherence and cell cycle of HT-29 colon carcinoma cells: evidence of sensitive and resistant sub-populations.
abstract: Diallyl disulfide (DADS) is a major organosulphur compound present in garlic with an anti-mitotic potential against colon neoplastic lesions in vivo and colon tumour cell growth in vitro. Using the human colon adenocarcinoma HT-29 Glc(-/+) cell line we identified sub-populations of tumoural cells with markedly different characteristics in terms of metabolic capacities, adhesion properties and distribution in the cell cycle phases. After 1 and 2 days treatment with 100 microM DADS HT-29 cells were largely released into the culture medium. These floating cells accumulated in the G(2)/M phase and were characterized by a 5-fold reduction in cell capacity for de novo protein synthesis. Polyamine metabolism, which is necessary for intestinal epithelial cell attachment and growth, was also severely affected, since 3-fold reductions in polyamine biosynthesis and net accumulation of putrescine were measured after DADS treatment. However, oxidation of L-glutamine, the main precursor of the tricarboxylic acid cycle in these cells, and de novo synthesis of glutathione, a tripeptide involved in tumoural cell chemoresistance, were not affected by DADS treatment. In contrast, the adherent sub-population of HT-29 cells, although partially accumulated in G(2)/M phase, were characterized by unaffected metabolic capacities when compared with control cells except for putrescine accumulation, which was transiently decreased, and L-glutamine oxidation, which was increased 2-fold. DADS-resistant cells selected within 5 days were then able to proliferate at a similar rate to control untreated cells. The DADS-induced changes in HT-29 metabolic capacities, adhesion properties and the cell cycle are discussed from a causal perspective.
authors.affiliation: Laboratoire de Nutrition et Sécurité Alimentaire, Institut National de la Recherche Agronomique, 78350 Jouy-en-Josas, France.
authors.forename: V
authors.initials: V
authors.lastname: Robert
authors.forename: B
authors.initials: B
authors.lastname: Mouillé
authors.forename: C
authors.initials: C
authors.lastname: Mayeur
authors.forename: M
authors.initials: M
authors.lastname: Michaud
authors.forename: F
authors.initials: F
authors.lastname: Blachier
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism* / Q000473:pathology; D000498:Allyl Compounds / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D002462:Cell Membrane; D003110:Colonic Neoplasms / Q000378:metabolism* / Q000473:pathology; D005434:Flow Cytometry; D005737:Garlic / Q000737:chemistry; D019073:HT29 Cells; D006801:Humans; D010946:Plants, Medicinal; D013440:Sulfides / Q000494:pharmacology*
chemical_list: D000498:Allyl Compounds; D013440:Sulfides; C038491:allyl sulfide