Enzyme-linked immunosorbent assay. II. Quantitative assay of protein antigen, immunoglobulin G, by means of enzyme-labelled antigen and antibody-coated tubes.
Alkaline phosphatase from calf intestinal mucosa has been conjugated to a protein antigen, rabbit IgG. Such conjugates, prepared by glutardialdehyde, have been used in a competitive solid phase immunoassay. In this test native antigen inhibits the binding of the conjugate to homologous antibodies adsorbed to plastic tubes. Using this assay 1-100 ng/ml of the antigen could be determined.
Engvall
E
E
Department of Immunology, Wenner-Gren Institute, University of Stockholm, Stockholm, Sweden.
Jonsson
K
K
Perlmann
P
P
D000327:Adsorption; D000469:Alkaline Phosphatase / Q000276:immunology*; D000818:Animals; D000906:Antibodies / Q000032:analysis*; D000918:Antibody Specificity; D000941:Antigens / Q000032:analysis*; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D007074:Immunoglobulin G; D007413:Intestinal Mucosa / Q000201:enzymology; D011817:Rabbits; D012756:Sheep
D000906:Antibodies; D000941:Antigens; D007074:Immunoglobulin G; D000469:Alkaline Phosphatase