Vitamin C decreases intracellular calcium level in human lymphoid cells.
Human lymphocytes have low levels of many antioxidant enzymes however they are know to concentrate vitamin C. Cell injury, including oxidative stress effects, is associated with calcium influx so the influence of vitamin C on the maintenance of calcium levels in leukocytes was studied. Incubation of Molt-3 human lymphoblastoid cells with physiologically relevant concentrations of vitamin C and the calcium ionophore A23187 reversed the calcium influx and increased nuclear protein level associated with the ionophore alone. It is concluded that intracellular vitamin C can inhibit calcium influx into leukocytes so helping to minimise cell damage.
Oztürk
G
G
University of Gazi, Faculty of Medicine, Department of Physiology, Beşevler-Ankara-Turkey. Ozturk@med.gazi.edu.tr
Mulholland
C W
CW
Hannigan
B M
BM
D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology*; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D002461:Cell Line, Transformed; D006801:Humans; D007424:Intracellular Fluid / Q000187:drug effects* / Q000378:metabolism; D007476:Ionophores / Q000494:pharmacology; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism*
D000975:Antioxidants; D007476:Ionophores; D000001:Calcimycin; D001205:Ascorbic Acid; D002118:Calcium