Identification of human CPI-17, an inhibitory phosphoprotein for myosin phosphatase.
CPI-17 is a phosphorylation-dependent inhibitor of myosin phosphatase. cDNA clones encoding CPI-17 were isolated from a human aorta library. Overlapping clones indicated two isoforms: CPI-17alpha was 147 residues and mass of 16.7 kDa; CPI-17beta (120 residues, mass 13.5 kDa) resulted from a deletion in the alpha-isoform of 27 residues, sequence 68-94. N-terminal 67 residues of all CPI-17 isoforms (human, porcine, rat and mouse) were highly conserved (for the human and porcine isoforms the identity was 91%). The presence of the two human isoforms was detected from cDNA sequences amplified by RT-PCR and by Western blots on human aorta. The cloned human CPI-17 gene indicated 4 coding exons and CPI-17beta was an alternative splice variant due to deletion of the second exon. FISH analysis located the human CPI-17 gene on chromosome 19q13.1.
Yamawaki
K
K
First Department of Internal Medicine, Mie University School of Medicine, Tsu, Mie, 514-8507, Japan.
Ito
M
M
Machida
H
H
Moriki
N
N
Okamoto
R
R
Isaka
N
N
Shimpo
H
H
Kohda
A
A
Okumura
K
K
Hartshorne
D J
DJ
Nakano
T
T
D000595:Amino Acid Sequence; D001011:Aorta / Q000737:chemistry; D001483:Base Sequence; D002874:Chromosome Mapping; D002888:Chromosomes, Human, Pair 19 / Q000235:genetics; D003001:Cloning, Molecular; D015723:Gene Library; D015698:Genomic Library; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000235:genetics*; D009131:Muscle, Smooth, Vascular / Q000737:chemistry; D043263:Myosin-Light-Chain Phosphatase; D010749:Phosphoprotein Phosphatases / Q000037:antagonists & inhibitors*; D010750:Phosphoproteins / Q000235:genetics*; D020033:Protein Isoforms; D017386:Sequence Homology, Amino Acid
D047908:Intracellular Signaling Peptides and Proteins; D009124:Muscle Proteins; C107507:PPP1R14A protein, human; D010750:Phosphoproteins; C491520:Ppp1r14a protein, mouse; C491519:Ppp1r14a protein, rat; D020033:Protein Isoforms; D010749:Phosphoprotein Phosphatases; D043263:Myosin-Light-Chain Phosphatase