[Changes in the state of phosphorylation of DARPP-32 of gerbil striatum during ischemia].
Using the mongolian gerbil model of transient forebrain ischemia by bilateral carotid arteries occlusion, the levels of phosphorylation and the amount of DARPP-32 in striatum were measured by back-phosphorylation and Western-blotting assay in vitro. Transient forebrain ischemia had no effect on the immunoreaction of DARPP-32 in striatum, but the state of phosphorylation of DARPP-32 showed a significant change. The [32P] phosphate incorporation in DARPP-32 decreased after 2, 7 or 10 min of ischemia, but increased after a 5-min ischemia. In contrast, the result of back-phosphorylation in vivo was opposite to that obtained in vitro.
Sun
Y F
YF
Research Center of Biochemistry and Molecular Biology, Xuzhou Medical College, Xuzhou 221002.
Tang
F M
FM
Wang
R
R
Zhang
G Y
GY
Jin
G Z
GZ
D000818:Animals; D002545:Brain Ischemia / Q000378:metabolism*; D003342:Corpus Striatum / Q000378:metabolism*; D051937:Dopamine and cAMP-Regulated Phosphoprotein 32; D005849:Gerbillinae; D009419:Nerve Tissue Proteins; D010750:Phosphoproteins / Q000378:metabolism*; D010766:Phosphorylation
D051937:Dopamine and cAMP-Regulated Phosphoprotein 32; D009419:Nerve Tissue Proteins; D010750:Phosphoproteins