title: [Investigation on the mechanics of adhesion to the selective extracellular matrix coated surfaces of lung cancer cells].
abstract: The adhesion properties of tumor cells with extracellular matrix(ECM) are closely associated with their invasion and metastasis. Our work reported here was intended reveal the relevant biomechanical and biorheological manifestations of human lung cancer. Using micropipette aspiration technique, we investigated quantitatively the adhesive mechanics properties of high metastatic human giant cell carcinoma(PG) cells as well as low metastatic adenocarcinoma(PAa) cells of lung based on cell culture in vitro. The results showed that the adhesion forces of PAa and PG cells to collagen IV were significantly higher than those to glass surfaces, but at the lower concentrations(1.00 microgram/ml and 2.00 micrograms/ml) of collagen IV, the amplitude for the increase of adhesion forces of PG cells were less than the amplitude for that of PAa cells, and most of the adhesion force values of PAa cells to the coated surfaces of incorporation of laminin along with 2 micrograms/ml collagen IV were significantly greater than those of PG cells. At the lower concentrations(0.625 microgram/ml for PAa cells, and 0.625 microgram/ml, 1.25 micrograms/ml for PG cells) of laminin tested, the adhesion force values of PAa and PG cells all decreased, but the amplitude and level for the decreased values of adhesion forces of PG cells were greater than those for the PAa cells. In conclusion, the adhesive and proteolytic behaviour of cancer cells to extracellular matrix might be mediated mainly by tumor cell membrane receptors such as integrin receptors and laminin receptors, it might affect the biological characteristics and the metastasis of the tumor cells. The results may benefit to explain some questions in biomechanical views about how the highly metastatic PG cells are prone to migration and invasion.
authors.affiliation: Second Hospital of Chongqing Medical University, Chongqing 400010.
authors.forename: T
authors.initials: T
authors.lastname: Zhang
authors.forename: Q
authors.initials: Q
authors.lastname: Qu
authors.forename: Y
authors.initials: Y
authors.lastname: Xue
authors.forename: Z
authors.initials: Z
authors.lastname: Wu
authors.forename: G
authors.initials: G
authors.lastname: Song
authors.forename: S
authors.initials: S
authors.lastname: Cai
mesh_terms: D000230:Adenocarcinoma / Q000473:pathology*; D018286:Carcinoma, Giant Cell / Q000473:pathology*; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology*; D003094:Collagen / Q000494:pharmacology; D016326:Extracellular Matrix Proteins / Q000494:pharmacology*; D006801:Humans; D007797:Laminin / Q000494:pharmacology; D008175:Lung Neoplasms / Q000473:pathology*; D014407:Tumor Cells, Cultured
chemical_list: D016326:Extracellular Matrix Proteins; D007797:Laminin; D003094:Collagen