title: Therapeutic efficacy of hepatitis B surface antigen-antibodies-recombinant DNA composite in HBsAg transgenic mice.
abstract: Therapeutic efficacy of HBsAg-anti-HBs-recombinant DNA harboring hepatitis B virus (HBV) S gene complex was compared with three other therapeutic vaccine candidates (recombinant HBsAg, HBsAg complexed to anti-HBs antibodies and naked plasmid DNA encoding the HBV S gene). After four injections at 3-week intervals, the most pronounced decrease of serum HBsAg, the highest titer of anti-HBs response, the highest level of interferon-gamma produced by splenocytes and potent cytotoxicity T cell response were observed in the HBsAg-anti HBs-sDNA immunized group. Reduced expression of HBsAg in hepatocytes was also shown. The therapeutic mechanism of HBsAg-anti-HBs-DNA was speculated as modulation of HBsAg presentation via both endogenous and exogenous pathways.
authors.affiliation: Department of Microbiology, The University of Hong Kong, Hong Kong, People's Republic of China.
authors.forename: B J
authors.initials: BJ
authors.lastname: Zheng
authors.forename: M H
authors.initials: MH
authors.lastname: Ng
authors.forename: L F
authors.initials: LF
authors.lastname: He
authors.forename: X
authors.initials: X
authors.lastname: Yao
authors.forename: K W
authors.initials: KW
authors.lastname: Chan
authors.forename: K Y
authors.initials: KY
authors.lastname: Yuen
authors.forename: Y M
authors.initials: YM
authors.lastname: Wen
mesh_terms: D000818:Animals; D000936:Antigen-Antibody Complex / Q000276:immunology*; D016207:Cytokines / Q000096:biosynthesis; D004274:DNA, Recombinant / Q000276:immunology*; D006509:Hepatitis B / Q000628:therapy*; D006510:Hepatitis B Antibodies / Q000276:immunology*; D006514:Hepatitis B Surface Antigens / Q000097:blood / Q000276:immunology*; D017325:Hepatitis B Vaccines / Q000627:therapeutic use*; D008099:Liver / Q000473:pathology / Q000821:virology; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology
chemical_list: D000936:Antigen-Antibody Complex; D016207:Cytokines; D004274:DNA, Recombinant; D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D017325:Hepatitis B Vaccines