title: The effect of circuit surface area on CD11b(mac-1) expression in a rat recirculation model.
abstract: The aim of the study was to assess the effect of exposed surface area of diethylhexylphthalate plasticized polyvinylchloride (PVC) on the expression of the adhesion molecule CD11b(mac-1) on neutrophils and to determine whether there is any apparent advantage in the current trend in reducing circuit surface area in terms of neutrophil activation. The study was carried out using a parallel plate rodent recirculation biomaterial testing model on 4 groups of 10 adult male Sprague Dawley rats weighing between 350 and 450 g. One group comprised the control group in which there was no biomaterial exposure. In the remaining 3 groups, the animals were subjected to either high (48 cm2), intermediate (24 cm2), or low (12 cm2) biomaterial surface area exposure. The parallel plate test cell was connected to the right femoral circulation and recirculation initiated at a flow rate of 1.5 ml/min for a period of 60 min. Blood samples were taken at 0, 30, and 60 min for the assessment of CD11b expression. Cd11b was assessed using flow cytometric analysis on neutrophils. The results demonstrated that there was a surface area related effect in the upregulation of CD11b. The difference at the terminal sample point between the highest surface area group (293.95 +/- 18.57%) and the low surface area group (133.80 +/- 49.31%) was highly statistically significant (p < 0.001). These results demonstrate that there may be some gain in terms of reduced inflammatory response from reducing the exposed surface area of PVC in extracorporeal perfusion circuits.
authors.affiliation: Department of Cardiac Surgery, National Heart and Lung Institute, Imperial College Medical School, Hammersmith Hospital, DuCane Road, London W12 ONN, United Kingdom. t.gourlay@ic.ac.uk
authors.forename: T
authors.initials: T
authors.lastname: Gourlay
authors.forename: D C
authors.initials: DC
authors.lastname: Stefanou
authors.forename: G
authors.initials: G
authors.lastname: Asimakopoulos
authors.forename: K M
authors.initials: KM
authors.lastname: Taylor
mesh_terms: D000818:Animals; D002315:Cardiopulmonary Bypass / Q000295:instrumentation*; D004051:Diethylhexyl Phthalate; D005434:Flow Cytometry; D016177:Macrophage-1 Antigen / Q000378:metabolism*; D008297:Male; D018375:Neutrophil Activation; D009504:Neutrophils / Q000378:metabolism*; D011143:Polyvinyl Chloride; D051381:Rats; D017207:Rats, Sprague-Dawley; D015854:Up-Regulation
chemical_list: D016177:Macrophage-1 Antigen; D011143:Polyvinyl Chloride; D004051:Diethylhexyl Phthalate