Sindbis virus variant with a deletion in the 6K gene shows defects in glycoprotein processing and trafficking: lack of complementation by a wild-type 6K gene in trans.
A Sindbis virus (SV) variant with a 6K gene partially deleted has been obtained. This SV Del6K virus is defective in the proteolytic processing of virus glycoprotein precursor, transport of glycoproteins to the plasma membrane, and plaque phenotype. A revertant virus (SV Del6K-revQ21L) containing a point mutation in the deleted 6K gene was isolated and characterized. SV Del6K-revQ21L has corrected the defects of proteolytic processing and transport of virus glycoproteins to the plasma membrane, but it still remains attenuated compared to wild-type (wt) SV, exhibiting defects in virus budding. Neither mutant nor revertant viruses are complemented by the coexpression in trans of a wt SV 6K gene.
Sanz
M A
MA
Centro de Biología Molecular, Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid, Spain. lcarrasco@cbm.uam.es
Carrasco
L
L
D018354:Alphavirus Infections / Q000821:virology*; D017353:Gene Deletion; D015967:Gene Expression Regulation, Viral; D006023:Glycoproteins / Q000235:genetics; D006801:Humans; D012845:Sindbis Virus / Q000502:physiology*; D014759:Viral Envelope Proteins / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics; D014779:Virus Replication / Q000235:genetics
C092026:6K protein, Sindbis virus; D006023:Glycoproteins; D014759:Viral Envelope Proteins; D014764:Viral Proteins