Agrobacterium tumefaciens-mediated transformation of rice with the spider insecticidal gene conferring resistance to leaffolder and striped stem borer.
Immature embryos of rice varieties "Xiushuill" and "Chunjiang 11" precultured for 4d were infected and transformed by Agrobacterium tumefaciens strain EHA101/pExT7 (containing the spider insecticidal gene). The resistant calli were transferred onto the differentiation medium and plants were regenerated. The transformation frequency reached 56% approximately 72% measured as numbers of Geneticin (G418)-resistant calli produced and 36% approximately 60% measured as numbers of transgenic plants regenerated, respectively. PCR and Southern blot analysis of transgenic plants confirmed that the T-DNA had been integrated into the rice genome. Insect bioassays using T1 transgenic plants indicated that the mortality of the leaffolder (Cnaphalocrasis medinalis) after 7d of leaf feeding reached 38% approximately 61% and the corrected mortality of the striped stem borer (Chilo suppressalis) after 7d of leaf feeding reached 16% approximately 75%. The insect bioassay results demonstrated that the transgenic plants expressing the spider insecticidal protein conferred enhanced resistance to these pests.
Huang
J Q
JQ
National Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai. jqhuang@iris.sipp.ac.cn
Wel
Z M
ZM
An
H L
HL
Zhu
Y X
YX
D016960:Agrobacterium tumefaciens / Q000235:genetics*; D000818:Animals; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D005818:Genetic Engineering / Q000379:methods*; D007113:Immunity, Innate / Q000235:genetics*; D007313:Insecta / Q000378:metabolism; D012275:Oryza / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics*; D012038:Regeneration / Q000235:genetics; D012098:Reproduction / Q000502:physiology; D013111:Spider Venoms / Q000235:genetics*; D014170:Transformation, Genetic / Q000502:physiology*
D013111:Spider Venoms