Levels of cellular glutathione and metallothionein affect the toxicity of oxidative stressors in an established carp cell line.
The comparative toxicity of a variety of oxidative stressors was studied in the epithelioma papulosum cyprini line from carp using the neutral red cytotoxicity assay. LC50's decreased in the order t-butylhydroperoxide > hydrogen peroxide > diquat > paraquat. The cytotoxicity of hydrogen peroxide was significantly reduced when the cells were grown in L-15 medium rather than MEM and this could be attributed to elevated cellular glutathione and metallothionein levels and higher activities of GSH-dependent detoxification systems. The protective effect of metallothionein in radical scavenging was demonstrated by decreased toxicity of the redox-cycling toxicants, diquat and menadione after metallothionein levels had been pre-induced by Cd-exposure. This study demonstrates the relationship between toxic effects of oxidative stressors and expression of detoxification systems in fish.
Wright
J
J
Institute of Aquaculture, University of Stirling, Stirling, Scotland FKL9 4LA, UK.
George
S
S
Martinez-Lara
E
E
Carpene
E
E
Kindt
M
M
D000818:Animals; D002347:Carps / Q000378:metabolism*; D002460:Cell Line; D004178:Diquat / Q000633:toxicity; D004347:Drug Interactions; D005978:Glutathione / Q000378:metabolism*; D006540:Herbicides / Q000633:toxicity*; D006861:Hydrogen Peroxide / Q000633:toxicity; D008668:Metallothionein / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects*; D010269:Paraquat / Q000633:toxicity; D010545:Peroxides / Q000633:toxicity*; D020122:tert-Butylhydroperoxide / Q000633:toxicity
D006540:Herbicides; D010545:Peroxides; D008668:Metallothionein; D020122:tert-Butylhydroperoxide; D004178:Diquat; D006861:Hydrogen Peroxide; D005978:Glutathione; D010269:Paraquat