title: Agrobacterium tumefaciens-mediated transformation of rice with the spider insecticidal gene conferring resistance to leaffolder and striped stem borer.
abstract: Immature embryos of rice varieties "Xiushuill" and "Chunjiang 11" precultured for 4d were infected and transformed by Agrobacterium tumefaciens strain EHA101/pExT7 (containing the spider insecticidal gene). The resistant calli were transferred onto the differentiation medium and plants were regenerated. The transformation frequency reached 56% approximately 72% measured as numbers of Geneticin (G418)-resistant calli produced and 36% approximately 60% measured as numbers of transgenic plants regenerated, respectively. PCR and Southern blot analysis of transgenic plants confirmed that the T-DNA had been integrated into the rice genome. Insect bioassays using T1 transgenic plants indicated that the mortality of the leaffolder (Cnaphalocrasis medinalis) after 7d of leaf feeding reached 38% approximately 61% and the corrected mortality of the striped stem borer (Chilo suppressalis) after 7d of leaf feeding reached 16% approximately 75%. The insect bioassay results demonstrated that the transgenic plants expressing the spider insecticidal protein conferred enhanced resistance to these pests.
authors.affiliation: National Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai. jqhuang@iris.sipp.ac.cn
authors.forename: J Q
authors.initials: JQ
authors.lastname: Huang
authors.forename: Z M
authors.initials: ZM
authors.lastname: Wel
authors.forename: H L
authors.initials: HL
authors.lastname: An
authors.forename: Y X
authors.initials: YX
authors.lastname: Zhu
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics*; D000818:Animals; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D005818:Genetic Engineering / Q000379:methods*; D007113:Immunity, Innate / Q000235:genetics*; D007313:Insecta / Q000378:metabolism; D012275:Oryza / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics*; D012038:Regeneration / Q000235:genetics; D012098:Reproduction / Q000502:physiology; D013111:Spider Venoms / Q000235:genetics*; D014170:Transformation, Genetic / Q000502:physiology*
chemical_list: D013111:Spider Venoms