A very efficient method to cleave Lipid A and saccharide components in bacterial lipopolysaccharides.
A novel mild procedure for the selective cleavage of ketosidic linkages is developed using ceric ammonium nitrate (CAN) in anhydrous N,N-dimethylformamide. Its application to lipopolysaccharides (LPS) is very significant because in the so far investigated LPS, the connection between the Lipid A region and the oligo(poly)saccharide part is always a keto-sugar. This procedure has been tested on LPS of Escherichia coli which contains Kdo as a linker between Lipid A and OPS and on Acinetobacter haemoliticus which contains D-glycero-D-talo-2-octulopyranosonic acid (Ko) as a linker and it performed efficiently in both cases.
Manzo
E
E
Dipartimento di Chimica Organica e Biochimica, Università di Napoli 'Federico II', via Cintia, I-80126, Naples, Italy.
Molinaro
A
A
Bedini
E
E
De Castro
C
C
Parrilli
M
M
D000150:Acinetobacter / Q000737:chemistry; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000737:chemistry*; D002563:Cerium; D004126:Dimethylformamide; D004926:Escherichia coli / Q000737:chemistry; D008050:Lipid A / Q000737:chemistry*; D008070:Lipopolysaccharides / Q000737:chemistry*; D008969:Molecular Sequence Data
D002241:Carbohydrates; D008050:Lipid A; D008070:Lipopolysaccharides; D002563:Cerium; D004126:Dimethylformamide; C004653:ceric ammonium nitrate