Identification and characterization of the UL24 gene product of herpes simplex virus type 2.
The UL24 gene of herpes simplex virus type 2 (HSV-2) is predicted to encode a 281 amino acid protein with a molecular mass of 30.5 kDa. In this study, the HSV-2 UL24 gene product has been identified by using a rabbit polyclonal antiserum produced against a recombinant protein containing the full-length UL24 gene product of HSV-2 fused to glutathione-S-transferase. The antiserum reacted specifically with a 32 kDa protein in HSV-2 186-infected Vero cells and with 31 and 32 kDa proteins in UL24-expressing Cos-7 cells. Accumulation of UL24 protein to detectable levels required viral DNA synthesis, indicating that the protein was regulated as a late gene. UL24 protein was found to be associated with purified HSV-2 virions and C capsids. Indirect immunofluorescence analysis demonstrated that the UL24-specific fluorescence was detected in perinuclear regions of the cytoplasm and/or in the nucleus as small discrete granules from 9h post infection (hpi). Furthermore, the UL24 protein expressed singly was detected predominantly in the nucleus and slightly in the cytoplasm at 24 h after transfection, with branch-like cytoplasmic protruding structures. Strong nucleolus staining was visible in partial cells.
Hong-Yan
Z
Z
Laboratory of Virology, Research Institute for Disease Mechanism and Control, Nagoya University School of Medicine, Japan.
Murata
T
T
Goshima
F
F
Takakuwa
H
H
Koshizuka
T
T
Yamauchi
Y
Y
Nishiyama
Y
Y
D000818:Animals; D001483:Base Sequence; D019556:COS Cells; D002213:Capsid / Q000378:metabolism; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D019084:Fluorescent Antibody Technique, Indirect; D018258:Herpesvirus 2, Human / Q000378:metabolism*; D007106:Immune Sera; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D014771:Virion / Q000378:metabolism
D017931:DNA Primers; D007106:Immune Sera; D014764:Viral Proteins