title: Occurrence of the major food allergen, ovomucoid, in human breast milk as an immune complex.
abstract: The major food allergen, ovomucoid (molecular weight of 28 kDa) could be detected in 12 of 37 human breast milk samples by using three types of enzyme-linked immunosorbent assay. By gel-filtration, ovomucoid in breast milk was only eluted in the fractions corresponding to a molecular weight of about 450 kDa, suggesting its occurrence as an immune complex with IgA. In fact, almost the same elution profile as that for ovomucoid was obtained for its immune complex with IgA by gel-filtration.
authors.affiliation: Research Institute for Food Science, Kyoto University, Uji, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Hirose
authors.forename: S
authors.initials: S
authors.lastname: Ito
authors.forename: N
authors.initials: N
authors.lastname: Hirata
authors.forename: S
authors.initials: S
authors.lastname: Kido
authors.forename: N
authors.initials: N
authors.lastname: Kitabatake
authors.forename: H
authors.initials: H
authors.lastname: Narita
mesh_terms: D000328:Adult; D000485:Allergens / Q000032:analysis*; D000818:Animals; D000936:Antigen-Antibody Complex / Q000737:chemistry*; D002850:Chromatography, Gel; D004531:Eggs / Q000032:analysis; D005260:Female; D005512:Food Hypersensitivity / Q000276:immunology*; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008895:Milk, Human / Q000737:chemistry*; D010059:Ovomucin / Q000032:analysis*; D051381:Rats
chemical_list: D000485:Allergens; D000936:Antigen-Antibody Complex; D010059:Ovomucin