Molecular cloning and sequence of gilthead sea bream (Sparus aurata) alpha-skeletal actin: tissue and developmental expression.
In the present study, the Sparus aurata alpha-skeletal actin was cloned from a mixed larvae complementary DNA library. The clone isolated was 1523 bp long with an open reading frame of 1134 bp coding for a 377-amino acid protein. The deduced amino acid sequence of sea bream alpha-actin is identical to Fugu alpha-actin-1. The expression of alpha-actin was initiated at the onset of segmentation. In adult fish, alpha-actin is expressed predominantly in white and red muscle.
Moutou
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Department of Biochemistry and Biotechnology, University of Thessaly, 26 Ploutonos Street, 41221, Larissa, Greece. kmoutou@uth.gr
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D000199:Actins / Q000096:biosynthesis* / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism; D005399:Fishes; D015723:Gene Library; D008969:Molecular Sequence Data; D009132:Muscles / Q000378:metabolism; D010802:Phylogeny; D012313:RNA / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D021541:Sea Bream; D017386:Sequence Homology, Amino Acid; D013997:Time Factors; D014018:Tissue Distribution
D000199:Actins; D018076:DNA, Complementary; D012313:RNA