Probing irradiated DNA with the perturbed angular correlation method.
The technique of perturbed angular correlations of gamma rays has been used to study the effects of radiation on DNA molecules. The samples are buffered solutions of calf thymus DNA exposed to various doses (0-80 Gy) of gamma rays. Indium-111 is used as a probe. Rotational correlation times, tau(c), a parameter measuring the flexibility of a macromolecule, are obtained that show a dependence on radiation dose.
Tsoulou
E
E
Institute of Nuclear Physics, NCSR Demokritos, 153 10 Agia Paraskevi, Athens, Greece.
Kalfas
C A
CA
Sideris
E G
EG
D000818:Animals; D002417:Cattle; D004247:DNA / Q000737:chemistry / Q000528:radiation effects*; D004249:DNA Damage; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays / Q000009:adverse effects; D066298:In Vitro Techniques; D008962:Models, Theoretical; D009686:Nuclear Physics; D009690:Nucleic Acid Conformation / Q000528:radiation effects; D013816:Thermodynamics
D004247:DNA