Differential-pulse adsorptive stripping voltammetric determination of the antibacterial lomefloxacin.
A differential-pulse adsorptive stripping voltammetric method for the determination of trace amounts of the antibacterial lomefloxacin is proposed. By using an accumulation potential of -0.30 V and a 2 min accumulation time, the linear concentration range of application was 1.0-10.0 ng ml(-1) of lomefloxacin, with a relative standard deviation of 3.8% (for a level of 5.0 ng ml(-1)) and a detection limit of 0.3 ng ml(-1). The method was applied to determination of lomefloxacin in human urine and serum samples. It was validated using HPLC as a reference method. Recovery levels of the method reached 100% in all cases.
Vílchez
J L
JL
Department of Analytical Chemistry, Faculty of Sciences, University of Granada, C/. Fuentenueva s/n, E-18071 Granada, Spain.
Araujo
L
L
Prieto
A
A
Navalón
A
A
D000890:Anti-Infective Agents / Q000097:blood / Q000652:urine*; D000892:Anti-Infective Agents, Urinary / Q000097:blood / Q000652:urine*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D024841:Fluoroquinolones; D006801:Humans; D006863:Hydrogen-Ion Concentration; D015363:Quinolones / Q000097:blood / Q000652:urine*
D000890:Anti-Infective Agents; D000892:Anti-Infective Agents, Urinary; D024841:Fluoroquinolones; D015363:Quinolones; C053091:lomefloxacin