Apaf-1 localization is modulated indirectly by Bcl-2 expression.
Apoptotic protease activating factor-1 (Apaf-1) is an adaptor molecule essential for caspase-9 activation. Subcellular analysis of Apaf-1 in NIH-3T3 fibroblasts and the immature murine B cell lymphoma WEHI-231 indicates that Apaf-1 is localized in the Golgi apparatus and cytoplasm. Bcl-2 overexpression in WEHI-231 cells disrupts Apaf-1 localization in Golgi, causing a perinuclear Apaf-1 redistribution. Bcl-2 overexpression in NIH-3T3 fibroblasts however does not cause similar Apaf-1 redistribution, suggesting that cell type factors are involved in the redistribution process. The ability of Bcl-2 to modify Apaf-1 subcellular localization is not explained by direct interaction between Apaf-1 and Bcl-2. These data may help to clarify the anti-apoptotic Bcl-2 function.
Ruiz-Vela
A
A
Department of Immunology and Oncology, Centro Nacional de Biotecnología/CSIC, Universidad Autónoma de Madrid, Campus de Cantoblanco, E-28049, Madrid, Spain. aruiz@cnb.uam.es
Albar
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JP
Martínez
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CA
D016475:3T3 Cells; D000818:Animals; D053477:Apoptotic Protease-Activating Factor 1; D019556:COS Cells; D003593:Cytoplasm / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D015870:Gene Expression; D019254:Genes, bcl-2 / Q000235:genetics; D006056:Golgi Apparatus / Q000378:metabolism; D006801:Humans; D051379:Mice; D009928:Organ Specificity; D011485:Protein Binding; D021381:Protein Transport; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism*; D014162:Transfection; D014407:Tumor Cells, Cultured
C506592:APAF1 protein, human; C506593:Apaf1 protein, mouse; D053477:Apoptotic Protease-Activating Factor 1; D011506:Proteins; D019253:Proto-Oncogene Proteins c-bcl-2