[Pathways of inclusion of isotopes 2H and 13C into exometabolites in course of glucose utilization by medusomycete].
The inclusion of 2H and 13C isotopes into the products of glucose utilization by medusomycete during its growth on deuterated media was studied by high-resolution NMR spectroscopy. Both unlabeled and 13C-labeled (in positions 1, 2, 6) glucose was used. It was shown that the glucose utilization proceeds by the classical Embden-Meyerhof-Parnas pathway. The incorporation of deuterium to the methyl group of ethanol can occur only during glucose-fructose-6-phosphate and phosphoenolpyruvate--pyruvate conversion. None of these stages by themselves is responsible for the existing distribution of deuterium atoms. The maximum inclusion of deuterium to the methyl group is no more than two atoms for the first glucose fragment (C1-C2-C3) and no more than one, for the second fragment (C4-C5-C6). The methylene group of ethanol is more accessible for deuterons because the proton surroundings of carbon atoms C2 and C5 completely changes. It was concluded that the maximum proton exchange occurs at positions C2 and C5; at positions C1, the proton exchange is lesser, and at position C6 it is the least. It was also shown that about 10% C1-C3 of triose leave the glycolysis cycle and are used in other processes.
Iurkevich
D I
DI
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, 142290 Russia.
Kutyshenko
V P
VP
D002247:Carbon Isotopes; D003903:Deuterium; D005658:Fungi / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D006019:Glycolysis; D009682:Magnetic Resonance Spectroscopy; D013662:Tea / Q000382:microbiology*
D002247:Carbon Isotopes; D013662:Tea; D003903:Deuterium; D005947:Glucose