Differentiation between inducers of apoptosis and nonspecific cytotoxic drugs by means of cell analyzer and immunoassay.
Differentiation between specific and nonspecific cytotoxic drugs by means of cell analyzer and immunoassay is described. The determination of antiproliferative, cytotoxic, and apoptotic effects as well as enlargement of cells in response to toxic compounds can be used for the disclosure of compounds with a specific mode of action. The usefulness of the procedure was demonstrated with camptothecin (1) as a cytotoxic drug and established inducer of apoptosis. Aphidicolin (2) and the new compound oxoaphidicolin (3) were shown to display no comparable effect. Isolation and structural data of 3 are reported.
Dahse
H M
HM
Hans-Knöll-Institute for Natural Products Research, Jena, Germany.
Schlegel
B
B
Gräfe
U
U
D000970:Antineoplastic Agents / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D000998:Antiviral Agents / Q000494:pharmacology; D016590:Aphidicolin / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D002166:Camptothecin / Q000494:pharmacology; D002452:Cell Count; D002455:Cell Division / Q000187:drug effects; D048429:Cell Size; D003584:Cytological Techniques; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007118:Immunoassay; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D000972:Antineoplastic Agents, Phytogenic; D000998:Antiviral Agents; D016590:Aphidicolin; D002166:Camptothecin