Molecular cloning and characterization of cel2 from the fungus Cochlibolus carbonum.
A new cellulase gene, cel2, from the filamentous fungus Cochliobolus carbonum was cloned by using egl-1 of Trichoderma reesei as a heterologous probe. DNA blot analysis of cel2 showed that this gene is present as a single copy. The gene contains one 49-bp- intron. cel2 encodes a predicted protein (Cel2p) of 423 amino acids with a molecular mass of 45.8 kDa. The predicted pI is 4.96. It shows similarity to other endoglucanases from various fungi. From the comparison with other cellulase genes, cel2 belongs to family 7 of glucohydrolases. cel2 is located on a 2.5-Mb chromosome in C. carbonum and its expression is repressed by sucrose. A cel2 mutant of C. carbonum was created by transformation-mediated gene disruption. The pathogenicity of the mutant was indistinguishable from the wild type, indicating that cel2 by itself is not important for pathogenicity.
Ahn
J H
JH
Department of Forest Resource, Konkuk University, Seoul, Korea. jhahn@konkuk.ac.kr
Sposato
P
P
Kim
S I
SI
Walton
J D
JD
D000595:Amino Acid Sequence; D001483:Base Sequence; D002482:Cellulose / Q000737:chemistry; D043366:Cellulose 1,4-beta-Cellobiosidase; D003001:Cloning, Molecular; D015247:Deoxyribonuclease HindIII / Q000737:chemistry / Q000378:metabolism; D005656:Fungal Proteins; D005658:Fungi / Q000737:chemistry* / Q000472:pathogenicity; D006026:Glycoside Hydrolases / Q000096:biosynthesis* / Q000737:chemistry; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000382:microbiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014242:Trichoderma / Q000737:chemistry / Q000235:genetics; D003313:Zea mays / Q000382:microbiology
D005656:Fungal Proteins; D002482:Cellulose; D015247:Deoxyribonuclease HindIII; D006026:Glycoside Hydrolases; C104579:CEL2 protein, Agaricus bisporus; D043366:Cellulose 1,4-beta-Cellobiosidase