Role of auxin-induced reactive oxygen species in root gravitropism.
We report our studies on root gravitropism indicating that reactive oxygen species (ROS) may function as a downstream component in auxin-mediated signal transduction. A transient increase in the intracellular concentration of ROS in the convex endodermis resulted from either gravistimulation or unilateral application of auxin to vertical roots. Root bending was also brought about by unilateral application of ROS to vertical roots pretreated with the auxin transport inhibitor N-1-naphthylphthalamic acid. Furthermore, the scavenging of ROS by antioxidants (N-acetylcysteine, ascorbic acid, and Trolox) inhibited root gravitropism. These results indicate that the generation of ROS plays a role in root gravitropism.
Joo
J H
JH
Center for Cell Signaling Research, Department of Biological Sciences, Ewha Womans University, Seoul 120-750, South Korea.
Bae
Y S
YS
Lee
J S
JS
D016166:Free Radical Scavengers / Q000378:metabolism; D018522:Gravitropism; D007210:Indoleacetic Acids / Q000502:physiology*; D010797:Phthalimides / Q000494:pharmacology; D018517:Plant Roots / Q000502:physiology*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D003313:Zea mays / Q000502:physiology*
D016166:Free Radical Scavengers; D007210:Indoleacetic Acids; D010797:Phthalimides; D017382:Reactive Oxygen Species; C002687:alpha-naphthylphthalamic acid