Preparation of oligonucleotides without aldehyde abasic sites.
High-quality oligonucleotides are obtained by selective modification of sequences containing aldehyde apurinic sites with a new chromatographic tag followed by RP-HPLC separation. Hydroxylamine derivative 1 of a water soluble nonionic surfactant modifies oligonucleotides selectively at abasic sites leading to significantly increased retention.
Krotz
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Isis Pharmaceuticals Inc., 2292 Faraday Avenue, 92008, Carlsbad, CA, USA. akrotz@isisph.com
Gaus
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Ravikumar
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Cole
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D000447:Aldehydes / Q000737:chemistry*; D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D019811:Hydroxylamine / Q000737:chemistry; D009841:Oligonucleotides / Q000737:chemistry* / Q000302:isolation & purification*; D011687:Purines / Q000737:chemistry*; D017384:Sequence Deletion / Q000502:physiology; D021241:Spectrometry, Mass, Electrospray Ionization; D013501:Surface-Active Agents / Q000737:chemistry*; D014867:Water / Q000737:chemistry
D000447:Aldehydes; D009841:Oligonucleotides; D011687:Purines; D013501:Surface-Active Agents; D014867:Water; D019811:Hydroxylamine