title: Antibacterial activity of black myrobalan (Terminalia chebula Retz) against Helicobacter pylori.
abstract: The effect of ether, alcoholic and water extracts of black myrobalan (Teminalia chebula Retz) on Helicobactor pylori were examined using an agar diffusion method on Columbia Agar. Water extracts of black myrobalan showed significant antibacterial activity and had a minimum inhibitory concentration (MIC) and minimum bacteriocidal concentration (MBC) of 125 and 150 mg/l, respectively. The extract was active after autoclaving for 30 min at 121 degrees C. Plant powder (incorporated in agar) gave higher MIC and MBC values (150 and 175 mg/l, respectively). Water extracts of the black myrobalan at a concentration of 1-2.5 mg/ml inhibited urease activity of H. pylori. The results show that black myrobalan extracts contain a heat stable agent(s) with possible therapeutic potential. Other bacterial species were also inhibited by black myrobalan water extracts.
authors.affiliation: Department of Microbiology and Biological Sciences, University of Tehran, Tehran, Iran.
authors.forename: F
authors.initials: F
authors.lastname: Malekzadeh
authors.forename: H
authors.initials: H
authors.lastname: Ehsanifar
authors.forename: M
authors.initials: M
authors.lastname: Shahamat
authors.forename: M
authors.initials: M
authors.lastname: Levin
authors.forename: R R
authors.initials: RR
authors.lastname: Colwell
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D006090:Gram-Negative Bacteria / Q000187:drug effects; D016480:Helicobacter pylori / Q000187:drug effects* / Q000201:enzymology; D006358:Hot Temperature; D007492:Iran; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000494:pharmacology; D010946:Plants, Medicinal; D019685:Rosales; D014510:Urease / Q000037:antagonists & inhibitors
chemical_list: D000900:Anti-Bacterial Agents; D010936:Plant Extracts; D014510:Urease