One plant actin isovariant, ACT7, is induced by auxin and required for normal callus formation.
During plant growth and development, the phytohormone auxin induces a wide array of changes that include cell division, cell expansion, cell differentiation, and organ initiation. It has been suggested that the actin cytoskeleton plays an active role in the elaboration of these responses by directing specific changes in cell morphology and cytoarchitecture. Here we demonstrate that the promoter and the protein product of one of the Arabidopsis vegetative actin genes, ACT7, are rapidly and strongly induced in response to exogenous auxin in the cultured tissues of Arabidopsis. Homozygous act7-1 mutant plants were slow to produce callus tissue in response to hormones, and the mutant callus contained at least two to three times lower levels of ACT7 protein than did the wild-type callus. On the other hand, a null mutation in ACT2, another vegetative actin gene, did not significantly affect callus formation from leaf or root tissue. Complementation of the act7-1 mutants with the ACT7 genomic sequence restored their ability to produce callus at rates similar to those of wild-type plants, confirming that the ACT7 gene is required for callus formation. Immunolabeling of callus tissue with actin subclass-specific antibodies revealed that the predominant ACT7 is coexpressed with the other actin proteins. We suggest that the coexpression, and probably the copolymerization, of the abundant ACT7 with the other actin isovariants in cultured cells may facilitate isovariant dynamics well suited for cellular responses to external stimuli such as hormones.
Kandasamy
M K
MK
Department of Genetics, Life Sciences Building, University of Georgia, Athens, GA 30602, USA.
Gilliland
L U
LU
McKinney
E C
EC
Meagher
R B
RB
D000199:Actins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D000911:Antibodies, Monoclonal; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D046508:Culture Techniques; D018506:Gene Expression Regulation, Plant; D005816:Genetic Complementation Test; D007210:Indoleacetic Acids / Q000378:metabolism*; D005810:Multigene Family; D009154:Mutation; D010802:Phylogeny; D018515:Plant Leaves / Q000166:cytology / Q000235:genetics; D010940:Plant Proteins; D018517:Plant Roots / Q000166:cytology / Q000235:genetics; D030821:Plants, Genetically Modified; D020033:Protein Isoforms / Q000235:genetics / Q000502:physiology
D000199:Actins; D000911:Antibodies, Monoclonal; D007210:Indoleacetic Acids; D010940:Plant Proteins; D020033:Protein Isoforms