Coupling of a supercritical fluid chromatography system to a hybrid (Q-TOF 2) mass spectrometer: on-line accurate mass measurements.
This note describes a simple and economical method to couple a supercritical fluid chromatography (SFC) system (Berger Instruments, US) with a high-resolution hybrid mass spectrometer (Q-TOF 2; Micromass, UK). This experimental arrangement has three distinct advantages: (1) coupling between the two systems can be effected without the need for an interface or hardware modifications of either system, (ii) this experimental arrangement provides on-line accurate mass SFC/MS measurements which are indispensable for the characterisation of new chemical entities and unknown metabolites, and (iii) the characteristically fast spectral acquisition rate of the time-of-flight (TOF) analyser renders the present arrangement an important contribution to future semipreparative fraction collection setups which use mass spectrometry as a detector.
Garzotti
M
M
GlaxoSmithKline Group, Glaxo Wellcome SpA, Medicines Research Centre, Via Fleming 4, 37135 Verona, Italy.
Rovatti
L
L
Hamdan
M
M
D000900:Anti-Bacterial Agents / Q000032:analysis; D002245:Carbon Dioxide / Q000032:analysis; D002853:Chromatography, Liquid / Q000379:methods*; D004917:Erythromycin / Q000032:analysis; D006854:Hydrocortisone / Q000032:analysis; D018942:Macrolides; D013058:Mass Spectrometry; D009827:Oleandomycin / Q000032:analysis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation / Q000379:methods*; D013739:Testosterone / Q000032:analysis; D014217:Troleandomycin / Q000032:analysis
D000900:Anti-Bacterial Agents; D018942:Macrolides; D002245:Carbon Dioxide; D013739:Testosterone; D004917:Erythromycin; D014217:Troleandomycin; C003145:picromycin; D009827:Oleandomycin; D006854:Hydrocortisone