Dominant bovine ovarian follicular cysts express increased levels of messenger RNAs for luteinizing hormone receptor and 3 beta-hydroxysteroid dehydrogenase delta(4),delta(5) isomerase compared to normal dominant follicles.
The objective was to compare ovarian steroids and expression of mRNAs encoding cytochrome P450 side-chain cleavage, cytochrome P450 17 alpha-hydroxylase, cytochrome P450 aromatase, 3 beta-hydroxysteroid dehydrogenase Delta(4),Delta(5) isomerase, LH, and FSH receptors and estrogen receptor-beta in ovaries of cows with dominant and nondominant ovarian follicular cysts and in normal dominant follicles. Estradiol-17 beta, progesterone, and androstenedione concentrations were determined in follicular fluid using specific RIAs. Dominant cysts were larger than young cysts or dominant follicles, whereas nondominant cysts were intermediate. Estradiol-17 beta (ng/ml) and total steroids (ng/follicle) were higher in dominant cysts than in dominant follicles. Expression of LH receptor and 3 beta-hydroxysteroid dehydrogenase mRNAs was higher in granulosa cells of dominant cysts than in dominant follicles. Nondominant cysts had higher follicular concentrations of progesterone, lower estradiol-17 beta concentrations, and lower expression of steroidogenic enzyme, gonadotropin receptor, and estrogen receptor-beta mRNAs than other groups. In summary, increased expression of LH receptor and 3 beta-hydroxysteroid dehydrogenase mRNAs in granulosa and increased follicular estradiol-17 beta concentrations were associated with dominant cysts compared to dominant follicles. Study of cysts at known developmental stages is useful in identifying alterations in follicular steroidogenesis.
Calder
M D
MD
Department of Animal Sciences, University of Missouri-Columbia, Columbia, Missouri 65211-5300, USA.
Manikkam
M
M
Salfen
B E
BE
Youngquist
R S
RS
Lubahn
D B
DB
Lamberson
W R
WR
Garverick
H A
HA
D015096:3-Hydroxysteroid Dehydrogenases / Q000235:genetics*; D000735:Androstenedione / Q000032:analysis; D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000378:metabolism*; D002786:Cholesterol Side-Chain Cleavage Enzyme / Q000235:genetics; D004958:Estradiol / Q000032:analysis; D005260:Female; D005497:Follicular Cyst / Q000378:metabolism / Q000662:veterinary*; D015571:Follicular Fluid / Q000737:chemistry; D006107:Granulosa Cells / Q000737:chemistry; D006080:Ovarian Follicle / Q000737:chemistry; D010052:Ovariectomy; D011374:Progesterone / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis*; D011974:Receptors, LH / Q000235:genetics*
D012333:RNA, Messenger; D011974:Receptors, LH; D000735:Androstenedione; D011374:Progesterone; D004958:Estradiol; D015096:3-Hydroxysteroid Dehydrogenases; D002786:Cholesterol Side-Chain Cleavage Enzyme