Size of donor chromosome segments around introgressed loci and reduction of linkage drag in marker-assisted backcross programs.
This article investigates the efficiency of marker-assisted selection in reducing the length of the donor chromosome segment retained around a locus held heterozygous by backcrossing. First, the efficiency of marker-assisted selection is evaluated from the length of the donor segment in backcrossed individuals that are (double) recombinants for two markers flanking the introgressed gene on each side. Analytical expressions for the probability density function, the mean, and the variance of this length are given for any number of backcross generations, as well as numerical applications. For a given marker distance, the number of backcross generations performed has little impact on the reduction of donor segment length, except for distant markers. In practical situations, the most important parameter is the distance between the introgressed gene and the flanking markers, which should be chosen to be as closely linked as possible to the introgressed gene. Second, the minimal population sizes required to obtain double recombinants for such closely linked markers are computed and optimized in the context of a multigeneration backcross program. The results indicate that it is generally more profitable to allow for three or more successive backcross generations rather than to favor recombinations in early generations.
Hospital
F
F
Station de Génétique Végétale, INRA/UPS/INAPG, 91190 Gif sur Yvette, France. fred@moulon.inra.fr
D002875:Chromosomes; D003433:Crosses, Genetic; D008040:Genetic Linkage; D006579:Heterozygote; D012641:Selection, Genetic