Stability of prostate specific antigen (PSA), and subsequent Y-STR typing, of Lucilia (Phaenicia) sericata (Meigen) (Diptera: Calliphoridae) maggots reared from a simulated postmortem sexual assault.
Rape-homicide represents one of the most heinous crimes, but which are also the hardest to solve due to the high occurrence of stranger-to-stranger interaction. This is the first case of obtaining P30 and Y-STR typing from a simulated postmortem sexual assault. 2, 3.5 and 6 microl of liquid semen was added to a liver substrate and Lucilia (Phaenicia) sericata (Meigen) eggs added. The larvae fed upon the semen coated substrate and were removed for testing after 48 and 145h after initial liquid semen deposition. P30 was recorded from whole postfeeding larvae after 145h, with correct Y-STR profiles obtained from the crop of actively feeding second instar larvae after 48h of initial semen deposition. The ability to obtain P30 and Y-STR profiles from larvae infesting a cadaver, with the suspicion of sexual assault having occurred prior to death, provides a new avenue to aid in the solving of such crimes.
Clery
J M
JM
Office of the Chief Medical Examiner, Department of Forensic Biology, 520 First Avenue, New York, NY 10016, USA. jamesclery@hotmail.com
D000328:Adult; D000483:Alleles; D000818:Animals; D004247:DNA / Q000145:classification; D004175:Diptera / Q000378:metabolism*; D005260:Female; D005554:Forensic Medicine / Q000379:methods*; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D017430:Prostate-Specific Antigen / Q000302:isolation & purification*; D011902:Rape; D020080:Tandem Repeat Sequences
D004247:DNA; D017430:Prostate-Specific Antigen