Rapid detection of multiple nucleopolyhedroviruses using polymerase chain reaction.
A technique using the polymerase chain reaction (PCR) was developed for detection of the nucleopolyhedrovirus (NPV) polyhedrin gene. The amino acid sequences of the polyhedrin gene were compared in twenty-six NPVs. A highly conserved DNA sequence within the coding region of the polyhedrin gene was targeted for amplification. One pair of degenerate PCR primers was designed to produce fragments of about 430 bp. The NPVs detected by this technique were Autographa californica NPV, Bombyx mori NPV, Hyphantria cunea NPV, Spodoptera exigua NPV, S. litura NPV, and Lymantria dispar NPV. This technique would be useful in monitoring the distribution of NPVs and release of the wild type and recombinant NPVs.
Woo
S D
SD
School of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Suwon, Korea. sdwoo68@hanmail.net
D000595:Amino Acid Sequence; D000818:Animals; D017124:Conserved Sequence; D017931:DNA Primers; D004279:DNA, Viral / Q000032:analysis; D007313:Insecta; D008969:Molecular Sequence Data; D017924:Nucleopolyhedroviruses / Q000235:genetics* / Q000302:isolation & purification*; D000077183:Occlusion Body Matrix Proteins; D016133:Polymerase Chain Reaction / Q000379:methods*; D014764:Viral Proteins / Q000235:genetics*; D015678:Viral Structural Proteins
D017931:DNA Primers; D004279:DNA, Viral; D000077183:Occlusion Body Matrix Proteins; D014764:Viral Proteins; D015678:Viral Structural Proteins; C009829:polyhedrin protein, Nucleopolyhedrovirus