Modulation of acid secretion in common bile duct ligation-related gastropathy in Wistar rats.
BACKGROUND
Portal hypertensive gastropathy is associated with fundic gland atrophy, resulting in a decrease in chief and parietal cells, and diminished acid secretion.


METHODS
Acid secretion by isolated parietal cells was measured (acridine orange retention), along with the levels of various second messengers (intracellular Ca(2+), cyclic adenosine monophosphate and protein kinase C) in the common bile duct, ligated portal hypertensive rats and compared with sham-operated controls.


RESULTS
There was a significant decrease in the response of isolated parietal cells to the secretagogues histamine and carbachol. This resulted in the blunted acid secretion in the common bile duct ligated group. In addition, all the second messengers studied were significantly decreased as compared with the sham-operated controls.


CONCLUSIONS
These results suggest that the blunted acid secretory response in the portal hypertensive rat is caused by an alteration in the intracellular signal transduction mechanism.
Kaur
S
S
Department of Experimental Medicine and Biotechnology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
Kaur
U
U
Agnihotri
N
N
Tandon
C D
CD
Majumdar
S
S
D000165:Acridine Orange / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002217:Carbachol / Q000494:pharmacology; D019872:Chief Cells, Gastric / Q000473:pathology; D003135:Common Bile Duct; D000242:Cyclic AMP / Q000378:metabolism; D005744:Gastric Acid / Q000378:metabolism*; D005748:Gastric Fundus / Q000473:pathology*; D006632:Histamine / Q000494:pharmacology; D006975:Hypertension, Portal / Q000473:pathology*; D008297:Male; D010295:Parietal Cells, Gastric / Q000473:pathology; D011493:Protein Kinase C / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015290:Second Messenger Systems / Q000502:physiology; D015398:Signal Transduction / Q000502:physiology
D006632:Histamine; D002217:Carbachol; D000242:Cyclic AMP; D011493:Protein Kinase C; D000165:Acridine Orange; D002118:Calcium