title: Diversity and genetic structure in populations of Pseudotsuga menziesii (Pinaceae) at chloroplast microsatellite loci.
abstract: Genetic variation was compared between uniparentally-inherited (chloroplast simple sequence repeats, cpSSRs) vs. biparentally-inherited (isozyme and random amplified polymorphic DNA, RAPD) genetic markers in Douglas-fir (Pseudotsuga mensiezii) from British Columbia. Three-hundred twenty-three individuals from 11 populations were assayed. In Douglas-fir, the cpSSR primer sites were well-conserved relative to Pinus thunbergii (11 of 17 loci clearly amplified), but only 3 loci were appreciably polymorphic. At these cpSSR loci, we found an unexpectedly low level of polymorphism within populations, and no genetic differentiation among populations. By contrast, the nuclear markers showed variation typical of conifers, with significant among-population differentiation. This difference is likely the outcome of both historical factors and high pollen dispersal.
authors.affiliation: Laboratoire Génétique et Evolution des Populations Vegetales, Centre National de la Recherche Scientifique (UPRESA-CNRS) 8016, Fédération de Recherche, Univ Lille 1, Villeneuve d'Ascq, France. viard@sb-roscoff.fr
authors.forename: F
authors.initials: F
authors.lastname: Viard
authors.forename: Y A
authors.initials: YA
authors.lastname: El-Kassaby
authors.forename: K
authors.initials: K
authors.lastname: Ritland
mesh_terms: D000483:Alleles; D001955:British Columbia; D002467:Cell Nucleus / Q000235:genetics; D032462:Cycadopsida / Q000235:genetics*; D018742:DNA, Chloroplast / Q000235:genetics*; D005787:Gene Frequency; D014644:Genetic Variation / Q000235:genetics*; D006239:Haplotypes; D018895:Microsatellite Repeats / Q000235:genetics*; D016296:Mutagenesis / Q000235:genetics; D011058:Pollen / Q000502:physiology; D011110:Polymorphism, Genetic / Q000235:genetics*; D019105:Random Amplified Polymorphic DNA Technique; D014197:Trees / Q000235:genetics*
chemical_list: D018742:DNA, Chloroplast