In silico cloning of a new protein kinase, Aik2, related to Drosophila Aurora using the new tool: EST Blast.
In this short communication we report for the first time to our knowledge the use of ESTBlast to in silico clone a new gene and a step by step description of this particular in silico cloning project.
Prigent
C
C
Departement de Biologie et Genetique du Developpement, CNRS UPR41, Université de Rennes I, 35042, Rennes, France.
Gill
R
R
Trower
M
M
Sanseau
P
P
D000595:Amino Acid Sequence; D000818:Animals; D064107:Aurora Kinase B; D064127:Aurora Kinases; D003001:Cloning, Molecular / Q000379:methods*; D016208:Databases, Factual; D004330:Drosophila / Q000201:enzymology* / Q000235:genetics*; D020224:Expressed Sequence Tags; D006801:Humans; D020407:Internet; D051379:Mice; D008969:Molecular Sequence Data; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D051381:Rats; D017386:Sequence Homology, Amino Acid; D012984:Software
C576921:AURKB protein, human; D064107:Aurora Kinase B; D064127:Aurora Kinases; D017346:Protein Serine-Threonine Kinases