HPLC determination of phenylpropanolamine in pharmaceutical preparations using 4-dimethylaminobenzaldehyde as a derivatizing reagent.
A method is described for the high performance liquid chromatographic (HPLC) determination of phenylpropanolamine (PPA) based on precolumn derivatization with 4-dimethylaminobenzaldehyde (DAB) and elution from phenomenex C-18 column with methanol-water and detection by spectrophotometry at 418 nm. Linear calibration was obtained with 9.4--46.9 microg ml(-1) with a detection limit of 4.7 ng ml(-1). Vitamin B(12) and rifampicin when present together with PPA separated completely and could be determined simultaneously. PPA was determined in pharmaceutical preparations with a relative standard deviation of 0.6--1.6%.
Rind
F M
FM
Institute of Chemistry, University of Sindh, Jamshoro Sindh, Pakistan.
Khuhawar
M Y
MY
Rajper
A D
AD
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004396:Coloring Agents / Q000032:analysis*; D004791:Enzyme Inhibitors / Q000032:analysis; D007202:Indicators and Reagents; D004364:Pharmaceutical Preparations / Q000032:analysis; D010665:Phenylpropanolamine / Q000032:analysis*; D013566:Sympathomimetics / Q000032:analysis*; D014805:Vitamin B 12 / Q000032:analysis; D004124:p-Dimethylaminoazobenzene / Q000032:analysis*
D004396:Coloring Agents; D004791:Enzyme Inhibitors; D007202:Indicators and Reagents; D004364:Pharmaceutical Preparations; D013566:Sympathomimetics; D010665:Phenylpropanolamine; D004124:p-Dimethylaminoazobenzene; D014805:Vitamin B 12