4-Acetyl-12,13-epoxyl-9-trichothecene-3,15-diol isolated from the fruiting bodies of Isariajaponica Yasuda induces apoptosis of human leukemia cells (HL-60).
The fruiting bodies of Isaria fungi have been traditionally used in Korea to treat cancer. An apoptosis-inducing compound, 4-acetyl-12,13-epoxyl-9-trichothecene-3,15-diol, was isolated from the methanol extract of fruiting bodies of Isaria japonica Yasuda by bioassay-guided fractionation. The apoptosis of the human leukemia cells (HL-60) by the compound was accessed by propidium iodide-staining flow cytometric analysis, and apoptosis-inducing activity at IC50 concentration (10 nmol/l) was further confirmed by a nuclear morphological change, a ladder pattern of internucleosomal DNA fragmentation, and an activation of caspase-3.
Oh
G S
GS
Medicinal Resources Research Center of Wonkwang University, Iksan, Chonbuk, South Korea.
Hong
K H
KH
Oh
H
H
Pae
H O
HO
Kim
I K
IK
Kim
N Y
NY
Kwon
T O
TO
Shin
M K
MK
Chung
H T
HT
D017209:Apoptosis / Q000187:drug effects*; D004396:Coloring Agents; D053938:DNA Fragmentation / Q000187:drug effects; D004273:DNA, Neoplasm / Q000187:drug effects; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D018922:HL-60 Cells; D006801:Humans; D006999:Hypocreales / Q000737:chemistry*; D007211:Indoles; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D009504:Neutrophils / Q000187:drug effects; D013778:Tetrazolium Salts; D013844:Thiazoles; D014255:Trichothecenes / Q000302:isolation & purification / Q000494:pharmacology*
C440202:4-acetyl-12,13-epoxy-9-trichothecene-3,15-diol; D004396:Coloring Agents; D004273:DNA, Neoplasm; D007211:Indoles; D013778:Tetrazolium Salts; D013844:Thiazoles; D014255:Trichothecenes; C007293:DAPI; C022616:thiazolyl blue