Heat shock proteins, HSP25 and HSP70, and apoptosis in developing endocardial cushion of the mouse heart.
Formation of the atrioventricular channels and valves from the endocardial cushion occurs through growth and remodeling of the initial endocardial cushion. This process requires balanced coordination of proliferation and apoptosis by still unknown factors. To detect a possible role for the heat shock proteins 25 and 70 (HSP25 and HSP70) as apoptosis-associated proteins and differentiation factors in the development of the endocardial cushion, we analyzed their temporal and regional occurrence during cell proliferation and apoptosis in E11-E17 embryos. The distribution and timing of these events and factors were consistent with the hypothesis that HSP25 is related to myocardial development whereas HSP70 is related to differentiation of the endocardial cushion by cell proliferation and apoptosis.
Abdelwahid
E
E
Department of Pediatrics, University of Turku, 20520 Turku, Finland. eltyeb.abdelwahid@utu.fi
Eriksson
M
M
Pelliniemi
L J
LJ
Jokinen
E
E
D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D004699:Endocardium / Q000737:chemistry / Q000166:cytology* / Q000196:embryology; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000032:analysis*; D006360:Heat-Shock Proteins; D008648:Mesoderm / Q000166:cytology; D051379:Mice; D008815:Mice, Inbred Strains; D008854:Microscopy, Electron; D008938:Mitosis / Q000502:physiology; D018832:Molecular Chaperones; D018485:Muscle Fibers, Skeletal / Q000737:chemistry / Q000648:ultrastructure; D009363:Neoplasm Proteins / Q000032:analysis*; D011247:Pregnancy; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis
D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins; C486113:Hsbp1 protein, mouse; D018832:Molecular Chaperones; D009363:Neoplasm Proteins; D018809:Proliferating Cell Nuclear Antigen