Iron inhibits the nitric oxide synthesis elicited by asbestos in murine macrophages.
Crocidolite fibers stimulated nitric oxide synthase (NOS) activity and expression in glial and alveolar murine macrophages: this effect was inhibited by iron supplementation and enhanced by iron chelation. We suggest that in these cells crocidolite stimulates NOS expression by decreasing the iron bioavailability and activating an iron-sensitive transcription factor.
Aldieri
E
E
Department of Genetics, Biology, and Biochemistry, University of Torino, Via Santena, 5/bis-10126 Torino, Italy. dario.ghigo@unito.it
Ghigo
D
D
Tomatis
M
M
Prandi
L
L
Fenoglio
I
I
Costamagna
C
C
Pescarmona
G
G
Bosia
A
A
Fubini
B
B
D000818:Animals; D017638:Asbestos, Crocidolite / Q000494:pharmacology*; D002460:Cell Line; D003676:Deferoxamine / Q000494:pharmacology*; D005290:Ferric Compounds / Q000494:pharmacology*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D007700:Kinetics; D016676:Macrophages, Alveolar / Q000187:drug effects* / Q000378:metabolism*; D051379:Mice; D009457:Neuroglia / Q000187:drug effects / Q000378:metabolism; D009569:Nitric Oxide / Q000096:biosynthesis*; D019001:Nitric Oxide Synthase / Q000235:genetics* / Q000378:metabolism*; D052247:Nitric Oxide Synthase Type II; D009571:Nitrilotriacetic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology*
D005290:Ferric Compounds; D017638:Asbestos, Crocidolite; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse; D003676:Deferoxamine; D009571:Nitrilotriacetic Acid; C020326:ferric nitrilotriacetate