title: Expression of exo-polygalacturonases in Botrytis cinerea.
abstract: The pathogenic fungus, Botrytis cinerea, causing gray mold disease in a variety of plant species, secretes at least four polygalacturonases (PGs), cell wall degrading enzymes. Among them, we prepared polyclonal antibody against purified 66-kDa exo-PG in rabbit. Immunoblot analysis revealed that the antibody recognized two exo-PGs, 66 kDa and 70 kDa in molecular mass, secreted from B. cinerea cultured in the medium containing citrus pectin as a carbon source. By immunohistochemical analysis, the expression of exo-PGs was identified in cucumber leaves inoculated with spores of B. cinerea. The exo-PGs were observed 9 h after inoculation, and the amount of exo-PGs increased with time in the leaves. The exo-PGs were induced by polygalacturonic acid as well as its monomer, galacturonic acid, in vitro. The expression of 66-kDa exo-PG (exo-PG I) increased with time of culture, while 70-kDa exo-PG (exo-PG II) was transiently expressed soon after the start of culture. Therefore, exo-PGs might play an important role in pathogenesis at an early stage of infection as well as in tissue maceration of host plant.
authors.affiliation: Division of Life Science and Research Institute of Natural Science, College of Natural Science, Gyeongsang National University, Jinju 660-701, South Korea.
authors.forename: E
authors.initials: E
authors.lastname: Rha
authors.forename: H J
authors.initials: HJ
authors.lastname: Park
authors.forename: M O
authors.initials: MO
authors.lastname: Kim
authors.forename: Y R
authors.initials: YR
authors.lastname: Chung
authors.forename: C W
authors.initials: CW
authors.lastname: Lee
authors.forename: J W
authors.initials: JW
authors.lastname: Kim
mesh_terms: D020171:Botrytis / Q000201:enzymology* / Q000254:growth & development; D018553:Cucumis sativus / Q000382:microbiology; D003470:Culture Media; D004790:Enzyme Induction; D006026:Glycoside Hydrolases / Q000032:analysis / Q000096:biosynthesis* / Q000276:immunology / Q000302:isolation & purification; D006603:Hexuronic Acids / Q000494:pharmacology; D007106:Immune Sera; D015151:Immunoblotting; D007124:Immunoenzyme Techniques; D010368:Pectins / Q000494:pharmacology; D018515:Plant Leaves / Q000382:microbiology
chemical_list: D003470:Culture Media; D006603:Hexuronic Acids; D007106:Immune Sera; C007819:galacturonic acid; D010368:Pectins; D006026:Glycoside Hydrolases; C020136:exopolygalacturonase