Optimization of submerged culture conditions for the mycelial growth and exo-biopolymer production by Cordyceps militaris.
OBJECTIVE
The objective of the present study was to determine the optimal culture conditions for exo-biopolymer production by Cordyceps militaris in shake flask culture.


RESULTS
The optimal temperature and initial pH for both mycelial growth and exo-biopolymer production by Cordyceps militaris in shake flask culture were found to be 20 degrees C and 6.0, respectively. Sucrose (40 g x l(-1)) and corn steep powder (10 g x l(-1)) were the most suitable carbon and nitrogen source for both mycelial growth and exo-biopolymer production.


CONCLUSIONS
Under optimal culture conditions, the maximum exo-biopolymer concentration in a 5-l jar fermenter indicated 10.3 g x l(-1), which was approximately three times higher than that in shake flask culture.


CONCLUSIONS
This process can have a significant impact on the industrial scale when sucrose and corn steep powder were used as carbon and nitrogen source.
Park
J P
JP
Department of Biotechnology, Taegu University, Kyungsan, Kyungbuk, Korea.
Kim
S W
SW
Hwang
H J
HJ
Yun
J W
JW
D002244:Carbon / Q000378:metabolism; D003470:Culture Media; D005285:Fermentation; D005296:Ferrous Compounds / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D006999:Hypocreales / Q000254:growth & development* / Q000378:metabolism*; D008278:Magnesium Sulfate / Q000494:pharmacology; D009584:Nitrogen / Q000378:metabolism; D010710:Phosphates / Q000494:pharmacology; D011134:Polysaccharides / Q000096:biosynthesis*; D017680:Potassium Compounds / Q000494:pharmacology; D013395:Sucrose / Q000378:metabolism; D013696:Temperature
D003470:Culture Media; D005296:Ferrous Compounds; D010710:Phosphates; D011134:Polysaccharides; D017680:Potassium Compounds; C020748:ferrous sulfate; D013395:Sucrose; D002244:Carbon; D008278:Magnesium Sulfate; C013216:potassium phosphate; D009584:Nitrogen