Decreased expression of apM1 in omental and subcutaneous adipose tissue of humans with type 2 diabetes.
We have screened a subtracted cDNA library in order to identify differentially expressed genes in omental adipose tissue of human patients with Type 2 diabetes. One clone (#1738) showed a marked reduction in omental adipose tissue from patients with Type 2 diabetes. Sequencing and BLAST analysis revealed clone #1738 was the adipocyte-specific secreted protein gene apM1 (synonyms ACRP30, AdipoQ, GBP28). Consistent with the murine orthologue, apM1 mRNA was expressed in cultured human adipocytes and not in preadipocytes. Using RT-PCR we confirmed that apM1 mRNA levels were significantly reduced in omental adipose tissue of obese patients with Type 2 diabetes compared with lean and obese normoglycemic subjects. Although less pronounced, apM1 mRNA levels were reduced in subcutaneous adipose tissue of Type 2 diabetic patients. Whereas the biological function of apM1 is presently unknown, the tissue specific expression, structural similarities to TNFalpha, and the dysregulated expression observed in obese Type 2 diabetic patients suggest that this factor may play a role in the pathogenesis of insulin resistance and Type 2 diabetes.
Statnick
M A
MA
Lilly Research Laboratories, Lilly Corporate Center, Indianapolis, IN 46285-0424, USA. m.statnick@lilly.com
Beavers
L S
LS
Conner
L J
LJ
Corominola
H
H
Johnson
D
D
Hammond
C D
CD
Rafaeloff-Phail
R
R
Seng
T
T
Suter
T M
TM
Sluka
J P
JP
Ravussin
E
E
Gadski
R A
RA
Caro
J F
JF
D052242:Adiponectin; D000273:Adipose Tissue / Q000378:metabolism*; D000328:Adult; D001786:Blood Glucose / Q000032:analysis; D015992:Body Mass Index; D003094:Collagen / Q000235:genetics; D003920:Diabetes Mellitus / Q000097:blood / Q000235:genetics* / Q000378:metabolism; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000235:genetics* / Q000378:metabolism; D005215:Fasting; D015723:Gene Library; D006801:Humans; D007328:Insulin / Q000097:blood; D036341:Intercellular Signaling Peptides and Proteins; D008875:Middle Aged; D009765:Obesity / Q000097:blood / Q000235:genetics* / Q000378:metabolism; D009852:Omentum; D011506:Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012867:Skin; D013851:Thinness; D014158:Transcription, Genetic
D052242:Adiponectin; D001786:Blood Glucose; D007328:Insulin; D036341:Intercellular Signaling Peptides and Proteins; D011506:Proteins; D012333:RNA, Messenger; D003094:Collagen