Accumulation forms of Zn and Pb in Phaseolus vulgaris in the presence and absence of EDTA.
The internalized speciation of Zn and Pb in roots and leaves of Phaseolus vulgaris grown in zinc sulfate, zinc EDTA, lead nitrate, and lead EDTA solutions were studied by electron microscopy (Zn) and extended X-ray absorption fine structure (EXAFS) spectroscopy (Zn and Pb). Zn was predominantly present as Zn phosphate dihydrate in the roots and leaves of the plant regardless of its form in solution. Pb was predominantly found in the leaves as cerussite (lead carbonate) when the plant was grown in Pb nitrate solution and as a mixture of PbEDTA and an undetermined species in contact with PbEDTA solution. Therefore, Phaseolus vulgaris is able to dissociate totally (Zn) or partly (Pb) the two metal-EDTA complexes from the nutrient solution and to bind these metals in other forms.
Sarret
G
G
Environmental Geochemistry Group, LGIT-IRIGM, University of Grenoble and CNRS, BP 53, 38041 Grenoble, France. gsarret@ulf-grenoble.fr
Vangronsveld
J
J
Manceau
A
A
Musso
M
M
D'Haen
J
J
Menthonnex
J J
JJ
Hazemann
J L
JL
D002614:Chelating Agents / Q000737:chemistry*; D004492:Edetic Acid / Q000737:chemistry*; D007854:Lead / Q000493:pharmacokinetics*; D027805:Phaseolus / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D012989:Soil Pollutants / Q000493:pharmacokinetics*; D012995:Solubility; D014018:Tissue Distribution; D015032:Zinc / Q000493:pharmacokinetics*
D002614:Chelating Agents; D012989:Soil Pollutants; D007854:Lead; D004492:Edetic Acid; D015032:Zinc