title: Long-term preservation of strains of Burkholderia cepacia, Pseudomonas spp. and Stenotrophomonas maltophilia isolated from patients with cystic fibrosis.
abstract: Long-term preservation methods are important in the maintenance of bacteria for downstream research applications. Most clinical laboratories have only limited resources for archiving isolates and therefore require cost-effective and simple methods. An effective and cheap storage method using debrinated blood and maintenance at -80 degrees C is described.
authors.affiliation: Northern Ireland Public Health Laboratory, Department of Bacteriology, Belfast City Hospital, UK. jemoore@niphl.dnet.co.uk
authors.forename: J E
authors.initials: JE
authors.lastname: Moore
authors.forename: A B
authors.initials: AB
authors.lastname: Shaw
authors.forename: T
authors.initials: T
authors.lastname: Stanley
authors.forename: M J
authors.initials: MJ
authors.lastname: Crowe
authors.forename: J S
authors.initials: JS
authors.lastname: Elborn
mesh_terms: D001431:Bacteriological Techniques; D016956:Burkholderia cepacia / Q000502:physiology; D015925:Cryopreservation; D003550:Cystic Fibrosis / Q000382:microbiology*; D006801:Humans; D011549:Pseudomonas / Q000502:physiology; D013048:Specimen Handling; D020615:Stenotrophomonas maltophilia; D013997:Time Factors