Does leptin modulate immune and endocrine response in the time of LPS-induced acute inflammation?
OBJECTIVE
In many studies it has been reported, that leptin may play an important role not only in the regulation of food intake and body weight but can modify immune response. The aim of our study was to estimate the effects of the administration of exogenous leptin on serum concentration of proinflammatory cytokines (interleukin 6-IL 6 and tumor necrosis factor alpha-TNF alpha) and anti-inflammatory cytokine (interleukin 10 - IL 10) during LPS induced acute inflammation. We also estimated leptin's influence on pituitary, thyroid, adrenal and gonadal hormones in response to lipopolysaccharide (LPS) induced acute inflammation.


METHODS
Male rats Wistar-Kyoto were divided into four groups, which received respectively: placebo (0.9% NaCl), LPS, leptin and leptin with LPS. The TNF alpha and IL 6 serum concentrations were measured after 2 hours and IL 10 after 4 hours. The pituitary, thyroid, adrenal and gonadal hormones serum concentrations were measured after 2 and 4 hours. Cytokine concentrations were estimated using ELISA tests and hormones concentrations using RIA tests.


RESULTS
Leptin did not have an effect on both cytokine responses (proinflammatory and anti-inflammatory) in the time of LPS-induced acute inflammation. Leptin enhanced LPS-induced increasing of corticosterone secretion after 2 hours and decreased LPS-induced inhibition of testosterone secretion after 4 hours.


CONCLUSIONS
Leptin can modulate hormone response during LPS-induced acute inflammation.
Bik
W
W
Department of Internal Medicine, Endocrinology and Haematology Central Hospital of Ministry of Home Affairs and Administration, Woloska 137, 02-507 Warsaw, Poland.
Wolinska-Witort
E
E
Chmielowska
M
M
Rusiecka-Kuczalek
E
E
Baranowska
B
B
D000818:Animals; D003345:Corticosterone / Q000097:blood; D016207:Cytokines / Q000097:blood; D006728:Hormones / Q000097:blood*; D007249:Inflammation / Q000097:blood* / Q000139:chemically induced / Q000276:immunology*; D016753:Interleukin-10 / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D007700:Kinetics; D020738:Leptin / Q000008:administration & dosage / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000008:administration & dosage*; D007986:Luteinizing Hormone / Q000097:blood; D008297:Male; D011388:Prolactin / Q000097:blood; D051381:Rats; D011921:Rats, Inbred WKY; D013739:Testosterone / Q000097:blood; D013974:Thyroxine / Q000097:blood; D014284:Triiodothyronine / Q000097:blood; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
D016207:Cytokines; D006728:Hormones; D015850:Interleukin-6; D020738:Leptin; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D014284:Triiodothyronine; D016753:Interleukin-10; D013739:Testosterone; D011388:Prolactin; D007986:Luteinizing Hormone; D013974:Thyroxine; D003345:Corticosterone