The development of novel mouse monoclonal antibodies against the CC531 rat colon adenocarcinoma.
In this paper we describe 4 new monoclonal antibodies to be applied in rat models for cancer. The monoclonal antibodies were obtained by immunizing Balb/c mice with CC531 rat colon adenocarcinoma cells. Hybridomas were produced and 4 were selected for their reactivity with CC531 in vitro (MG1, 2, 3 and 4). All 4 antibodies recognized other rat tumour cell lines and showed limited cross-reactivity with normal rat tissues. Intraperitoneally injected MG1, 2 and 4 homed to in vivo growing, artificially induced CC531 liver metastases. In these in vivo experiments, limited cross-reactivity with normal rat tissues, predominantly of the gastro-intestinal tract, was found. MG4 was found to enhance lysis of CC531 tumour cells mediated by IL-2 activated, cultured natural killer cells. These antibodies are potentially useful for antibody-based laboratory techniques and for investigation of antibody-based immunotherapy of cancer in a rat model.
Hagenaars
M
M
Department of Surgery, Leiden University Medical Center, The Netherlands.
Koelemij
R
R
Ensink
N G
NG
van Eendenburg
J D
JD
van Vlierberghe
R L
RL
Eggermont
A M
AM
van de Velde
C J
CJ
Fleuren
G J
GJ
Kuppen
P J
PJ
D000230:Adenocarcinoma / Q000276:immunology* / Q000628:therapy; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000627:therapeutic use; D000918:Antibody Specificity; D015153:Blotting, Western; D003110:Colonic Neoplasms / Q000276:immunology* / Q000628:therapy; D003429:Cross Reactions; D006825:Hybridomas / Q000276:immunology; D008114:Liver Neoplasms, Experimental / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009928:Organ Specificity; D051381:Rats
D000911:Antibodies, Monoclonal