Dietary fat type and regular exercise affect mitochondrial composition and function depending on specific tissue in the rat.
Physical exercise and fatty acids have been studied in relation to mitochondrial composition and function in rat liver, heart, and skeletal muscle. Male rats were divided into two groups according to dietary fat type (virgin olive and sunflower oils). One-half of the animals from each group were subjected to a submaximal exercise for 8 weeks; the other half acted as sedentary controls. Coenzyme Q, cytochromes b, c + c1, a + a3 concentrations, and the activity of cytochrome c oxidase were determined. Regular exercise increased (P < 0.05) the concentration of the above-mentioned elements and the activity of the cytochrome c oxidase by roughly 50% in liver and skeletal muscle. In contrast, physical exercise decreased (P < 0.05) cytochrome c oxidase activity in the heart (in micromol/min/g, from 8.4+/-0.1 to 4.9+/-0.1 in virgin olive oil group and from 9.7+/-0.1 to 6.7+/-0.2 in sunflower oil animals). Dietary fat type raised the levels of coenzyme Q, cytochromes, and cytochrome c oxidase activity in skeletal muscle (P < 0.05) among the rats fed sunflower oil. In conclusion, dietary fat type, regular exercise, and the specific tissue modulate composition and function of rat mitochondria.
Quiles
J L
JL
Institute of Nutrition and Food Technology, Department of Physiology, University of Granada, Spain. jlquiles@goliat.ugr.es
Huertas
J R
JR
Mañas
M
M
Ochoa
J J
JJ
Battino
M
M
Mataix
J
J
D000818:Animals; D003580:Cytochromes / Q000378:metabolism; D004042:Dietary Fats, Unsaturated / Q000008:administration & dosage* / Q000032:analysis; D003576:Electron Transport Complex IV / Q000378:metabolism; D005227:Fatty Acids / Q000032:analysis; D008297:Male; D008928:Mitochondria / Q000378:metabolism*; D008929:Mitochondria, Heart / Q000378:metabolism; D008930:Mitochondria, Liver / Q000378:metabolism; D008931:Mitochondria, Muscle / Q000378:metabolism; D018482:Muscle, Skeletal / Q000378:metabolism; D000069463:Olive Oil; D005082:Physical Exertion / Q000502:physiology*; D010938:Plant Oils / Q000008:administration & dosage / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D000074242:Sunflower Oil; D014018:Tissue Distribution; D014451:Ubiquinone / Q000378:metabolism
D003580:Cytochromes; D004042:Dietary Fats, Unsaturated; D005227:Fatty Acids; D000069463:Olive Oil; D010938:Plant Oils; D000074242:Sunflower Oil; D014451:Ubiquinone; D003576:Electron Transport Complex IV