[Removal of the 3'- and 5'-untranslated region amplifies expression of the erythropoietin gene in mammalian cells].
The effect of the 5T- and 3T-untranslated regions (UTR) of human erythropoietin gene (gEpo) on its expression in mammalian cells was investigated. 5T-UTR-deleted, 3T-UTR-deleted, and 5T,3T-UTR-deleted gEpos were transfected into COS-7 cells, and the amount of transiently expressed Epo was measured. Cells transfected with the 5T,3T-UTR-deleted gEpo showed the highest expression, 2.6 times that with wild-type gEpo. Northern blot analysis indicated that the enhanced expression of 5T,3T-UTR-deleted gEpo might be related with the transcription efficiency.
Park
D Kh
DKh
Kim
C
C
Kim
W B
WB
Kim
Y K
YK
Lee
S Y
SY
Yang
J M
JM
D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D000818:Animals; D001483:Base Sequence; D019556:COS Cells; D017931:DNA Primers; D018076:DNA, Complementary; D004921:Erythropoietin / Q000235:genetics*; D005786:Gene Expression Regulation / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics; D014158:Transcription, Genetic
D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D017931:DNA Primers; D018076:DNA, Complementary; D012333:RNA, Messenger; D004921:Erythropoietin