Large-scale separation of resveratrol, anthraglycoside A and anthraglycoside B from Polygonum cuspidatum Sieb. et Zucc by high-speed counter-current chromatography.
High-speed counter-current chromatography was successfully applied to the large-scale separation of resveratrol, anthraglycoside A and anthraglycoside B from the crude extract of Polygonum cuspidatum Sieb. et Zucc using a two-phase solvent system composed of chloroform, methanol and water. Resveratrol, anthraglycoside A and anthraglycoside B were separated from multigram quantities (5 g) of crude extract of P. cuspidatum. The separation yielded 200 mg to 1 g of these three compounds each at over 98% purity as determined by HPLC. The chemical structures of these components were identified by nuclear magnetic resonance (NMR) and MS.
Yang
F
F
Beijing Research Centre for Separation and Purification of Natural Products, Beijing Institute of New Technology Application, China.
Zhang
T
T
Ito
Y
Y
D000873:Anthracenes / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D003377:Countercurrent Distribution / Q000379:methods*; D006027:Glycosides / Q000302:isolation & purification*; D028502:Polygonum / Q000737:chemistry*; D000077185:Resveratrol; D013057:Spectrum Analysis; D013267:Stilbenes / Q000302:isolation & purification*
D000873:Anthracenes; D006027:Glycosides; D013267:Stilbenes; C442641:anthraglycoside A; D000077185:Resveratrol; C442642:anthraglycoside B