Identification of larval-stage antigens of ascaris suum recognized with immune sera from pigs.
Antigens were identified from the third-stage larvae (L3) and lung-stage larvae of Ascaris suum by two-dimensional immunoblot method with antisera obtained from pigs that received chemically abbreviated Ascaris suum larval infections. Forty-seven and 13 spots were recognized as antigens from the L3 and lung-stage larvae, respectively. Their apparent molecular weight ranged from 20 to 101 kDa and their isoelectric point from 3.6 to 8.0. The present study provides a framework for further molecular cloning of those antigens and consequently leads to the development of recombinant peptide vaccines against A. suum.
Kasug-Aoki
H
H
Laboratory of Parasitic Diseases, National Institute of Animal Health, Tsukuba, Ibaraki, Japan.
Tsuji
N
N
Suzuki
K
K
Arakawa
T
T
Matsumoto
Y
Y
Isobe
T
T
D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis*; D001196:Ascariasis / Q000097:blood / Q000276:immunology / Q000469:parasitology / Q000662:veterinary*; D017165:Ascaris suum / Q000254:growth & development* / Q000276:immunology; D015153:Blotting, Western / Q000662:veterinary; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D005260:Female; D007525:Isoelectric Focusing / Q000662:veterinary; D008297:Male; D013047:Specific Pathogen-Free Organisms; D013552:Swine; D013553:Swine Diseases / Q000097:blood / Q000276:immunology / Q000469:parasitology*
D000947:Antigens, Helminth