title: Dynamics of association of origins of DNA replication with the nuclear matrix during the cell cycle.
abstract: DNA of replication foci attached to the nuclear matrix was isolated from Chinese hamster ovary cells and human HeLa cells synchronized at different stages of the G(1) and S phases of the cell cycle. The abundance of sequences from dihydrofolate reductase ori-beta and the beta-globin replicator was determined in matrix-attached DNA. The results show that matrix-attached DNA isolated from cells in late G(1) phase was enriched in origin sequences in comparison with matrix-attached DNA from early G(1) phase cells. The concentration of the early firing ori-beta in DNA attached to the matrix decreased in early S phase, while the late firing beta-globin origin remained attached until late S phase. We conclude that replication origins associate with the nuclear matrix in late G(1) phase and dissociate after initiation of DNA replication in S phase.
authors.affiliation: Institute of Molecular Biology, Bulgarian Academy of Sciences, Akad. G. Bonchev Street, Bl. 21, Sofia 1113, Bulgaria.
authors.forename: V
authors.initials: V
authors.lastname: Djeliova
authors.forename: G
authors.initials: G
authors.lastname: Russev
authors.forename: B
authors.initials: B
authors.lastname: Anachkova
mesh_terms: D000818:Animals; D016466:CHO Cells; D002453:Cell Cycle; D002463:Cell Membrane Permeability; D006224:Cricetinae; D004247:DNA / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D004261:DNA Replication; D003850:Deoxyribonuclease I / Q000378:metabolism; D016193:G1 Phase; D005914:Globins / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D007700:Kinetics; D015530:Nuclear Matrix / Q000378:metabolism*; D017830:Octoxynol; D018741:Replication Origin / Q000235:genetics*; D016196:S Phase; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics
chemical_list: D017830:Octoxynol; D005914:Globins; D004247:DNA; D013762:Tetrahydrofolate Dehydrogenase; D003850:Deoxyribonuclease I