title: Selenomethionine content of candidate reference materials.
abstract: Selenium has been identified as an antioxidant of importance in the diet. Accurate determination of its chemical forms depends on the availability of suitable reference materials (RMs). Two candidate reference materials for determination of selenomethionine (Semet) in food-related materials, a standard wheat gluten sample (NIST RM 8418 Wheat Gluten) and a commercial selenium enriched yeast, have been examined by use of a gas chromatography-isotope dilution mass spectrometry (IDMS) procedure, after treatment of the matrix with 0.1 mol L(-1) hydrochloric acid containing stannous chloride, addition of CNBr, and extraction with chloroform. This procedure results in cleavage of the CH3Se group to form volatile CH3SeCN. Addition of isotopically enriched 74Semet to an analytical sample enables estimation of the naturally occurring protein-bound 80Semet by IDMS without a protein-digestion process. We found that the Wheat Gluten RM contains a significant amount of Semet as a portion of its assigned value of 2.58 microg Se(total g(-1). Commercial selenium yeast tablets are labeled as containing an elevated level of "organic selenium", usually as Semet. The sample we investigated contained 210 microg Se(total) g(-1) sample as determined separately by IDMS, measuring elemental selenium after digestion. 73% of this total (153 +/- 21 microg Se(semet) g(-1); n = 23) was present as Semet. Thus, these two materials contain significant amounts of their total selenium content as Semet and would be good candidates for further study and characterization as reference materials for determining this important food component. The CNBr reaction used will also enable the determination of Se-(methyl)selenocysteine, the biological role of which is of recent interest. In addition to matrix RMs for Semet, it is important to have standard materials of the pure substance. We have examined a sample of a candidate standard material of selenomethionine being prepared by the USP. It was confirmed that this material is pure selenomethionine.
authors.affiliation: Food Composition Laboratory, BHNRC, ARS, USDA, Beltsville, MD 20705, USA. wolf@bhnrc.usda.gov
authors.forename: W R
authors.initials: WR
authors.lastname: Wolf
authors.forename: H
authors.initials: H
authors.lastname: Zainal
authors.forename: B
authors.initials: B
authors.lastname: Yager
mesh_terms: D003080:Cold Temperature; D003488:Cyanogen Bromide / Q000737:chemistry; D004355:Drug Stability; D005504:Food Analysis / Q000379:methods / Q000592:standards; D008401:Gas Chromatography-Mass Spectrometry; D005983:Glutens / Q000032:analysis; D007553:Isotope Labeling; D007554:Isotopes; D012015:Reference Standards; D012643:Selenium / Q000737:chemistry; D012645:Selenomethionine / Q000032:analysis*; D014908:Triticum / Q000737:chemistry*; D015002:Yeast, Dried / Q000737:chemistry*
chemical_list: D007554:Isotopes; D005983:Glutens; D012645:Selenomethionine; D012643:Selenium; D003488:Cyanogen Bromide