Depolymerization of the capsular polysaccharide from Vibrio cholerae O139 by a lyase associated with the bacteriophage JA1.
We have studied the interaction between the Vibrio cholerae O139 specific phage JA1, belonging to the Podoviridae family, and the capsular polysaccharide (CPS) of the parent strain from which the phage was isolated. Upon incubation of the JA1 phage with the CPS, oligosaccharides were isolated and purified. The oligosaccharides derived from one (shown below) and two repeating units of the CPS were characterized using NMR spectroscopy, mass spectrometry and sugar analysis (structure: see text). The cleavage was found to occur by beta-elimination at the 4-substituted alpha-linked galacturonic acid, which results in a 4-deoxy-beta-L-threo-hex-4-enopyranosyl uronic acid group (Sug). The enzyme associated with the JA1 phage responsible for the depolymerization of the V. cholerae O139 CPS is thus a lyase.
Linnerborg
M
M
Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, S-10691, Stockholm, Sweden.
Weintraub
A
A
Albert
M J
MJ
Widmalm
G
G
D001435:Bacteriophages / Q000201:enzymology*; D002240:Carbohydrate Sequence; D006868:Hydrolysis; D008190:Lyases / Q000378:metabolism*; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D009844:Oligosaccharides / Q000737:chemistry / Q000378:metabolism; D011108:Polymers / Q000378:metabolism; D011134:Polysaccharides / Q000378:metabolism*; D011135:Polysaccharides, Bacterial / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014734:Vibrio cholerae / Q000737:chemistry*
D009844:Oligosaccharides; D011108:Polymers; D011134:Polysaccharides; D011135:Polysaccharides, Bacterial; D008190:Lyases