Galactose dialdehyde: the forgotten candidate for a protein cross-linker?
Oxidation of D-galactose mediated by D-galactose oxidase [EC 1.1.3.9] gave a dialdehyde that occurs as two bicyclic structures in aqueous solution as established by 13C labeling and NMR spectroscopy. The dialdehyde is very reactive at basic pH leading to complex reaction mixtures. A comparison was made with glutaraldehyde to assess its potential as protein cross-linker.
Schoevaart
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R
Industrial Fermentative Chemistry, Leiden University, Einsteinweg 55, PO Box 9502, 2300 RA, Leiden, The Netherlands.
Kieboom
T
T
D000447:Aldehydes / Q000737:chemistry* / Q000378:metabolism; D003432:Cross-Linking Reagents / Q000737:chemistry* / Q000378:metabolism; D005690:Galactose / Q000737:chemistry* / Q000378:metabolism; D005976:Glutaral / Q000737:chemistry / Q000378:metabolism; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism
D000447:Aldehydes; D003432:Cross-Linking Reagents; D011506:Proteins; D005976:Glutaral; D005690:Galactose