Rat mitochondrial ATP synthase ATP5G3: cloning and upregulation in pancreas after chronic ethanol feeding.
Individuals with chronic excessive alcohol ingestion are put at the risk of acute and chronic pancreatitis. Underlying molecular mechanisms are unknown. Differential gene expression in the pancreas was profiled using mRNA differential display by comparison between control and ethanol-consuming rats. Male Wistar rats were fed with diets containing 6.7% (vol/vol) ethanol for 4 wk. A cDNA tag that was overexpressed in the pancreas of rats fed ethanol was isolated. A 723-bp cDNA was cloned from a rat pancreatic cDNA library, which encodes a novel rat mitochondrial ATP synthase subunit 9, isoform 3 (ATP5G3), which is homologous to a human ATP5G3 gene. Real-time PCR demonstrated that all three nuclear gene isoforms (ATP5G1, ATP5G2, and ATP5G3) were consistently upregulated in the pancreas of alcohol-consuming rats, parallel with mitochondrial injury. The cellular response to mitochondrial damage and metabolic stress may reflect an adaptive process for mitochondrial repair in pancreatic acinar cells during chronic ethanol ingestion.
Li
H S
HS
Department of Medicine, Center for Genomic Sciences, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA.
Zhang
J Y
JY
Thompson
B S
BS
Deng
X Y
XY
Ford
M E
ME
Wood
P G
PG
Stolz
D B
DB
Eagon
P K
PK
Whitcomb
D C
DC
D000595:Amino Acid Sequence; D000818:Animals; D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D000431:Ethanol / Q000008:administration & dosage / Q000494:pharmacology*; D005656:Fungal Proteins; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D008928:Mitochondria / Q000201:enzymology* / Q000648:ultrastructure; D025261:Mitochondrial Proton-Translocating ATPases / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D010179:Pancreas / Q000187:drug effects / Q000201:enzymology* / Q000648:ultrastructure; D019512:Pancreatitis, Alcoholic / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D017386:Sequence Homology, Amino Acid; D015854:Up-Regulation
D005656:Fungal Proteins; D012333:RNA, Messenger; D000431:Ethanol; D025261:Mitochondrial Proton-Translocating ATPases