Simple ion chromatographic method for the determination of chlormequat residues in pears.
Current methods for quantitative determination of chlormequat residues in food crops are characterized by rather low recoveries and the need for derivatization (in case of gas chromatography, GC), or by high capital investment (in case of liquid chromatography-mass spectrometry, LC-MS). We propose a cation-exchange chromatography method for the analysis of chlormequat in pears. The method is based on extraction of the target compound with 40 mM HCl, followed by centrifugation and filtration. The filtrate is directly injected into an ion chromatograph equipped with a commercially available cation-exchange column and a suppressed conductivity detection system. While the limit of detection (LOD) (0.5 mg/kg) may not be small enough to allow dietary analysis, the method meets all validation requirements and is an alternative for the existing GC and LC-MS methods in quality control.
Peeters
M H
MH
Teaching Support Unit, Faculty of Agricultural and Applied Biological Sciences, Katholieke Universiteit Leuven, Belgium. christine.peeters@agr.kuleuven.ac.be
Defloor
I
I
Coosemans
J
J
Delcour
J A
JA
Ooms
L
L
Deliever
R
R
De Vos
D
D
D002716:Chlormequat / Q000032:analysis*; D002852:Chromatography, Ion Exchange / Q000379:methods*; D005638:Fruit / Q000737:chemistry*; D010937:Plant Growth Regulators / Q000032:analysis*; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization
D010937:Plant Growth Regulators; D002716:Chlormequat