title: Free-flow electrophoresis of proteins in phenol-containing solvents at various pH values.
abstract: 1. Several proteins were found to migrate when subjected to free-flow electrophoresis in buffered phenol-ethanediol-water (3:2:3, w/v/v) solvent mixtures. Mobility of these proteins changed with changing pH (apparent) values of this medium. A pH value of zero mobility for each individual protein could be estimated. 2. Founded on these observations, a high-voltage electrophoresis method in free-flowing buffer films was worked out. The method as presented here was particularly suitable for the separation of proteins on a preparative scale. Application of this and other protein fractionation techniques in dissociating media for the investigation of structural and other insoluble proteins was discussed.
authors.affiliation: Rowett Research Institute, Bucksburn, Aberdeen, AB2 9SB, Great Britain.
authors.forename: A
authors.initials: A
authors.lastname: Pusztai
authors.forename: W B
authors.initials: WB
authors.lastname: Watt
mesh_terms: D000818:Animals; D002919:Chymotrypsinogen / Q000302:isolation & purification; D003207:Conalbumin / Q000302:isolation & purification; D003574:Cytochrome c Group / Q000302:isolation & purification; D004586:Electrophoresis / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D010434:Pepsin A / Q000302:isolation & purification; D010636:Phenols; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification*; D012710:Serum Albumin, Bovine / Q000302:isolation & purification; D012997:Solvents; D000509:alpha-Fetoproteins / Q000302:isolation & purification
chemical_list: D003574:Cytochrome c Group; D010636:Phenols; D011506:Proteins; D012997:Solvents; D000509:alpha-Fetoproteins; D003207:Conalbumin; D012710:Serum Albumin, Bovine; D002919:Chymotrypsinogen; D010434:Pepsin A