Molecular cloning and sequence analysis of the Zygosaccharomyces rouxiiLEU2 gene encoding a beta-isopropylmalate dehydrogenase.
A DNA fragment carrying the LEU2 gene of osmotolerant yeast Zygosaccharomyces rouxii was isolated. The sequenced DNA fragment (2630 bp) contained two ORFs; one of them (1086 bp long, predicting a protein of 362 amino acids) shared a high degree of similarity with LEU2 genes of other yeast species. The cloned DNA fragment fully complemented the leu2 mutations of Saccharomyces cerevisiae and Z. rouxii.
Sychrová
H
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Department of Membrane Transport, Institute of Physiology, CzAcadSci, 142 20 Prague 4, Czech Republic. sychrova@biomed.cas.cz
D050539:3-Isopropylmalate Dehydrogenase; D000429:Alcohol Oxidoreductases / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D005800:Genes, Fungal; D015698:Genomic Library; D008969:Molecular Sequence Data; D004718:Saccharomycetales / Q000201:enzymology / Q000235:genetics; D017422:Sequence Analysis, DNA; D020068:Zygosaccharomyces / Q000201:enzymology / Q000235:genetics*
D000429:Alcohol Oxidoreductases; D050539:3-Isopropylmalate Dehydrogenase