Malondialdehyde modification of proteins in vitro is enhanced in the presence of acetaldehyde.
To test the hypothesis that the cardioprotective effect of alcohol is related to the inhibition of malondialdehyde (MDA) modification of proteins by acetaldehyde (AA), we studied the effect of AA on MDA modification of bovine serum albumin (BSA) in vitro. BSA was incubated simultaneously with a fixed concentration of MDA (70 mM) and different concentrations of AA (120, 60, 30, 10, or 0 mM) for 24 h at 37 degrees C. The MDA-modified or AA-modified BSA was quantitated with immunoblotting by using specific anti-MDA and specific anti-AA protein antisera, respectively. In another set of experiments, BSA was incubated sequentially, first with different concentrations of AA and then with 70 mM of MDA. In both incubation protocols, the presence of AA and AA modification of BSA enhanced MDA binding. These in vitro observations suggest that the putative cardioprotective effects of alcohol or wine cannot be ascribed to AA-mediated reduction in MDA protein formation, a possible biochemical pathway of accelerated atherosclerosis.
Mooradian
A D
AD
Division of Endocrinology, Diabetes and Metabolism, Department of Internal Medicine, Saint Louis University School of Medicine, St. Louis, Missouri 63110, USA. mooradad@slu.edu
Reinacher
D
D
Li
J P
JP
Pinnas
J L
JL
D000079:Acetaldehyde / Q000008:administration & dosage / Q000378:metabolism*; D000438:Alcohols; D000818:Animals; D015153:Blotting, Western; D002417:Cattle; D004305:Dose-Response Relationship, Drug; D005609:Free Radicals; D006031:Glycosylation; D066298:In Vitro Techniques; D015227:Lipid Peroxidation / Q000502:physiology*; D008315:Malondialdehyde / Q000008:administration & dosage / Q000378:metabolism*; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism*; D012710:Serum Albumin, Bovine / Q000378:metabolism*
D000438:Alcohols; D005609:Free Radicals; D011506:Proteins; D012710:Serum Albumin, Bovine; D008315:Malondialdehyde; D000079:Acetaldehyde