title: Cellular localization of orexin receptors in human pituitary.
abstract: Orexins-A and -B are hypothalamic peptides derived from a precursor called prepro-orexin and relationated with the stimulation of food intake. They act on G protein receptors named orexin receptor 1 (OX(1)R) and orexin receptor 2 (OX(2)R), respectively. In the present study, we used RT-PCR and immunohistochemical techniques to detect the presence of OX(1)R and OX(2)R in human pituitary. A band of the expected size for both OX(1)R and OX(2)R was shown in human pituitary by RT-PCR. The cellular localization of OX(1)R and OX(2)R was carried out using histological techniques. By consecutive sections we demonstrated that OX(1)R was present in acidophil, diffusely distributed cells, which represent the half of the total adenohypophysis cell population. As was expected, these cells were shown to coexpress GH. OX(2)R was found in the pars intermedia and in clusters of basophil cells of the anterior pituitary, which coexpress ACTH. These results were confirmed by double immunofluorescence techniques. We also found focal positivity in axon terminals of neurohypophysis, more intense for OX(2)R than for OX(1)R. In conclusion, these results demonstrated for the first time that OX(1)R and OX(2)R were expressed by somatotrope and corticotrope cells, respectively.
authors.affiliation: Department of Morphological Sciences, School of Medicine-University Clinical Hospital, University of Santiago de Compostela, E-15782 Santiago de Compostela, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Blanco
authors.forename: M
authors.initials: M
authors.lastname: López
authors.forename: T
authors.initials: T
authors.lastname: GarcIa-Caballero
authors.forename: R
authors.initials: R
authors.lastname: Gallego
authors.lastname: Vázquez-Boquete A
authors.forename: G
authors.initials: G
authors.lastname: Morel
authors.forename: R
authors.initials: R
authors.lastname: SeñarIs
authors.forename: F
authors.initials: F
authors.lastname: Casanueva
authors.forename: C
authors.initials: C
authors.lastname: Diéguez
authors.forename: A
authors.initials: A
authors.lastname: Beiras
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D064446:Orexin Receptors; D010903:Pituitary Gland, Anterior / Q000166:cytology / Q000378:metabolism*; D010904:Pituitary Gland, Posterior / Q000166:cytology / Q000378:metabolism*; D043562:Receptors, G-Protein-Coupled; D018013:Receptors, Neuropeptide / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D064446:Orexin Receptors; D043562:Receptors, G-Protein-Coupled; D018013:Receptors, Neuropeptide