title: Micellar electrokinetic chromatography of polyamines and monoacetylpolyamines.
abstract: A selective procedure for qualitative and quantitative analysis of ten polyamines by micellar electrokinetic chromatography (MEKC) was developed. Benzoylated polyamines and acetylpolyamines in micellar phase of SDS (10 mM) were separated at 25 degrees C by 20 mM borate buffer pH 8.5, containing 8% ethanol, with an applied voltage of 25 kV (5 microA) and then detected at 198 nm. The experimental factors and operational parameters were optimized by performing analysis at different surfactant concentrations, pH, voltage and temperature with and without ethanol. The repeatibility of migration times and peak heights is a peculiarity of the method here described.
authors.affiliation: Institute of Biological Chemistry, University of Palermo, Italy. gtaibi@unipa.it
authors.forename: G
authors.initials: G
authors.lastname: Taibi
authors.forename: M R
authors.initials: MR
authors.lastname: Schiavo
authors.forename: P C
authors.initials: PC
authors.lastname: Rindina
authors.forename: R
authors.initials: R
authors.lastname: Muratore
authors.forename: C M
authors.initials: CM
authors.lastname: Nicotra
mesh_terms: D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D011073:Polyamines / Q000032:analysis*; D015203:Reproducibility of Results
chemical_list: D011073:Polyamines