title: PCR clamping.
abstract: An efficient, PCR based method for the selective amplification of DNA target sequences that differs by a single base pair is described. The method utilises the high affinity and specificity of PNA for their complementary nucleic acids and that PNA cannot function as primers for DNA polymerases.
authors.affiliation: PNA Diagnostics A/S, Copenhagen, Denmark.
authors.forename: H
authors.initials: H
authors.lastname: Orum
mesh_terms: D000483:Alleles; D020029:Base Pairing; D001667:Binding, Competitive; D017931:DNA Primers; D009691:Nucleic Acid Denaturation; D009695:Nucleic Acid Renaturation; D017354:Point Mutation; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D017931:DNA Primers