[Determination of D- and L- forms of aspartic acid in Achilles tendon tissue as an indicator of age].
The presence of the D-form of aspartic acid was detected in collagen of dentin and the intervertebral disk before and assessment of its racemization coefficient was used for assessment of the age of subjects. Another tissue formed almost solely by collagen type I are tendons. Tendon tissue was not investigated so far with regard to the racemization coefficient and therefore the authors assessed the D-form of aspartic acid in the Achilles tendon. They used lyophilized tendon excisions which were hydrolyzed. Amino acids in the hydrolysate were derived to N-TFA isopropyl esters which were assessed by gas chromatography on a chiral capillary column. In all samples the D-form of aspartic acid was detected. The values of the racemization coefficient suggest the possible use of thus processed tissue with certain limitations.
Pilin
A
A
Ustav soudního lékarství a toxikologie 1. LF UK a VFN, Praha. pilin@lf1.cuni.cz
Cabala
R
R
Pudil
F
F
Bencko
V
V
D000125:Achilles Tendon / Q000737:chemistry*; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000378:metabolism*; D001224:Aspartic Acid / Q000032:analysis*; D005554:Forensic Medicine / Q000379:methods*; D006801:Humans; D008875:Middle Aged
D001224:Aspartic Acid