title: [New knowledge regarding tumor cell inactivation and histological evaluation after radiofrequency therapy. Single case observation and in vitro proof of a new hypothesis].
abstract: In a study, a radiofrequency (RF) treatment was performed on a patient with a small breast cancer after vacuum biopsy. As usual in cases with a malignant diagnosis, surgical excision and axillary dissection followed. Histopathology revealed some residual tumor in the margin of the cavity. It could not be distinguished from vital tumor on the hematotoxylin eosin (HE) stain. Based on the correlation of MRI and histopathology after subsequent surgical excision, we did, however, presume that the residual was contained within the zone of inactivation. Thus the hypothesis arose that, if too high temperatures can be avoided, it might be possible to inactivate tumor cells without significantly impairing histopathologic assessment. This hypothesis was supported by the following in vitro experiment performed on a fresh specimen: An RF treatment was performed using temperatures up to 70 degrees C only. Half of the specimen underwent HE-staining, the other half vitality testing. The results indicate that if a given temperature range is strictly observed it appears possible to inactivate tissue before tissue sampling, since histopathologic diagnosis will not be impaired. Further technologic improvements may eventually allow to develop a pre-treatment method which might permit to avoid potential hematogenous tumor spread during subsequent biopsy.
authors.affiliation: Klinik für Diagnostische Radiologie, Universität Halle, Magdeburger Strasse 16, 06112 Halle. sylvia.heywang@medizin.uni-halle.de
authors.forename: S H
authors.initials: SH
authors.lastname: Heywang-Köbrunner
authors.forename: A
authors.initials: A
authors.lastname: Heinig
authors.forename: W
authors.initials: W
authors.lastname: Weiwad
authors.forename: D
authors.initials: D
authors.lastname: Lampe
authors.forename: H
authors.initials: H
authors.lastname: Kölbl
authors.forename: R P
authors.initials: RP
authors.lastname: Spielmann
authors.forename: J
authors.initials: J
authors.lastname: Buchmann
mesh_terms: D001706:Biopsy; D001940:Breast / Q000473:pathology; D001943:Breast Neoplasms / Q000473:pathology* / Q000601:surgery; D018270:Carcinoma, Ductal, Breast / Q000473:pathology* / Q000601:surgery; D002470:Cell Survival; D003131:Combined Modality Therapy; D005260:Female; D006801:Humans; D006979:Hyperthermia, Induced; D066298:In Vitro Techniques; D008875:Middle Aged; D018365:Neoplasm, Residual / Q000473:pathology* / Q000628:therapy; D011237:Predictive Value of Tests