title: Rat prominin, like its mouse and human orthologues, is a pentaspan membrane glycoprotein.
abstract: Mouse prominin is the first characterized member of a novel family of membrane glycoproteins. It displays a characteristic membrane topology with five transmembrane segments and two large glycosylated extracellular loops. Prominin orthologues and paralogues have been identified in human, fish, fly, and worm. Recently, a cDNA sequence encoding the rat homologue of mouse prominin has been reported [Zhu et al. (2001) Biochem. Biophys. Res. Commun. 281, 951-956]. Surprisingly, due to a single nucleotide deletion that shifts the reading frame and introduces a premature stop codon, the protein predicted from this cDNA would correspond to a C-terminally truncated form of prominin with only four transmembrane segments. Here we report evidence that is in contrast to the report of Zhu et al. (2001). We isolated a rat prominin cDNA devoid of any frameshift mutation, demonstrate that rat prominin, like the other mammalian prominins, is a full-length 120-kDa pentaspan membrane glycoprotein, and have not been able to detect any C-terminally truncated form of rat prominin.
authors.affiliation: Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, Dresden, D-01307, Germany.
authors.forename: D
authors.initials: D
authors.lastname: Corbeil
authors.forename: C A
authors.initials: CA
authors.lastname: Fargeas
authors.forename: W B
authors.initials: WB
authors.lastname: Huttner
mesh_terms: D000071916:AC133 Antigen; D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D006023:Glycoproteins; D006801:Humans; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D010455:Peptides; D016133:Polymerase Chain Reaction; D051381:Rats; D016364:Reading Frames; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D018076:DNA, Complementary; D006023:Glycoproteins; D008562:Membrane Glycoproteins; D010455:Peptides; C000606025:Prom1 protein, rat