title: An Alu cassette in the human epithelial sodium channel.
abstract: Here, we report the presence of two splice variants of the human epithelial sodium channel alpha subunit (h alpha ENaC) containing Alu cassette, namely h alpha ENaC+22 and h alpha ENaC+Alu, in various tissues. Functional expression of these splice variants with hENaC beta and gamma subunits produced loss-of-channel activity in the Xenopus oocyte expression system. Interestingly, coexpression of h alpha ENaC+22 or h alpha ENaC+Alu, respectively, with wild type hENaC alpha, beta, and gamma subunits enhanced the expression of amiloride-sensitive current in oocytes. The presence of Alu sequences in the 3'-untranslated region of h gamma ENaC was also identified.
authors.forename: Y S
authors.initials: YS
authors.lastname: Oh
authors.forename: S
authors.initials: S
authors.lastname: Lee
authors.forename: C
authors.initials: C
authors.lastname: Won
authors.forename: D G
authors.initials: DG
authors.lastname: Warnock
mesh_terms: D017398:Alternative Splicing; D020087:Alu Elements; D000595:Amino Acid Sequence; D001483:Base Sequence; D004848:Epithelium / Q000378:metabolism*; D006801:Humans; D008956:Models, Chemical; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D015222:Sodium Channels / Q000378:metabolism*
chemical_list: D015222:Sodium Channels