Optimization of carotenoid production from hyper-producing Rhodotorula glutinis mutant 32 by a factorial approach.
OBJECTIVE
Optimization of carotenoid production by a mutant of Rhodotorula glutinis.


METHODS
The growth and carotenoid production was optimized in shake flasks using a two-level, three-variable factorial design.


RESULTS
The volumetric carotenoid production could be increased to 129 +/- 2 mg x 1(-1) in a medium containing (g x l(-1)) yeast extract 11.74, glucose 46 and threonine 18 along with other micronutrients, wherein, beta-carotene yield was 102 +/- 2 mg x l(-1), accounting for 80% of the total carotenoids.


CONCLUSIONS
The medium optimization resulted in a fourfold increase in volumetric production and a twofold increase in the cellular accumulation of carotenoids. In view of such high yields, the mutant of Rhodotorula glutinis can be a potential source of beta-carotene.
Bhosale
P
P
Chemical Engineering Division, National Chemical Laboratory, Pune, India.
Gadre
R V
RV
D002338:Carotenoids / Q000096:biosynthesis*; D003470:Culture Media; D005947:Glucose / Q000378:metabolism; D009154:Mutation; D012248:Rhodotorula / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D013912:Threonine / Q000378:metabolism; D019207:beta Carotene / Q000096:biosynthesis
D003470:Culture Media; D019207:beta Carotene; D013912:Threonine; D002338:Carotenoids; D005947:Glucose