title: Antitumor Effect of Immunizations With Fusions of Dendritic and Glioma Cells in a Mouse Brain Tumor Model.
abstract: SUMMARY: The authors studied antitumor immunity conferred by fusions of dendritic and glioma cells in a mouse brain tumor model. Previous immunization with fusion cells (FCs) prevented tumor formation on challenge with glioma cells in the flank or in the brain. Efficacy was decreased when studies were performed in mice depleted of CD8+ cells. In a treatment model, FCs were injected subcutaneously after tumor development in the brain. The administration of FCs alone had limited effects on survival of mice bearing brain tumors. Importantly, however, administration of FCs and recombinant interleukin-12 (rIL-12) remarkably prolonged the survival of mice with brain tumors. Cytotoxic T lymphocyte activity against glioma cells from immunized mice was also stimulated by coadministration of FCs and rIL-12 compared with that obtained with FCs or rIL-12 alone. These data support the therapeutic efficacy of combining FC-based vaccine therapy and rIL-12.
authors.affiliation: Department of Oncology, The Institute of DNA Medicine, and Departments of Neurosurgery and Internal Medicine, Jikei University School of Medicine, Tokyo, Japan; and Dana-Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts, U.S.A.
authors.forename: Yasuharu
authors.initials: Y
authors.lastname: Akasaki
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Kikuchi
authors.forename: Sadamu
authors.initials: S
authors.lastname: Homma
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Abe
authors.forename: Donald
authors.initials: D
authors.lastname: Kofe
authors.forename: Tsuneya
authors.initials: T
authors.lastname: Ohno