title: Mutations on fibrinogen (gamma 316-322) are associated with reduction in platelet adhesion under flow conditions.
abstract: In this paper we report on studies of platelet adhesion to several fibrinogen gamma chain variants under physiological flow conditions. Reduced platelet adhesion was found to patient dysfibrinogen Vlissingen and its recombinant form (deletion of gamma 319-320). Furthermore, substitutions of the amino acids 318, 320, or both in the recombinant fibrinogen gamma chain showed a strong decrease in platelet adhesion under flow conditions in our perfusion system. Antibodies raised against peptides covering these sequences inhibited platelet adhesion completely, which suggested that the gamma 316-322 sequence could be involved in platelet adhesion in flowing blood.
authors.affiliation: Thrombosis and Haemostasis Laboratory, Department of Haematology, University Medical Center, Utrecht, P.O. Box 85500, 3508 GA Utrecht, The Netherlands. J.A.Remijn@lab.azu.nl
authors.forename: J A
authors.initials: JA
authors.lastname: Remijn
authors.forename: K C
authors.initials: KC
authors.lastname: Lounes
authors.forename: K A
authors.initials: KA
authors.lastname: Hogan
authors.forename: S T
authors.initials: ST
authors.lastname: Lord
authors.forename: D K
authors.initials: DK
authors.lastname: Galanakis
authors.forename: J J
authors.initials: JJ
authors.lastname: Sixma
authors.forename: P G
authors.initials: PG
authors.lastname: De Groot
mesh_terms: D000906:Antibodies / Q000276:immunology; D001792:Blood Platelets / Q000166:cytology*; D002448:Cell Adhesion / Q000276:immunology / Q000502:physiology*; D005340:Fibrinogen / Q000235:genetics* / Q000502:physiology*; D006801:Humans; D009154:Mutation; D010455:Peptides / Q000276:immunology
chemical_list: D000906:Antibodies; D010455:Peptides; D005340:Fibrinogen