title: Insulin resistance and type 2 diabetes are not related to resistin expression in human fat cells or skeletal muscle.
abstract: Resistin is secreted by rodent fat cells and was recently postulated to be an important link between obesity and insulin resistance. We examined Resistin gene expression with real-time RT-PCR in human isolated fat cells, adipose tissue, and muscle from 42 individuals of varying degrees of overweight and who had normal insulin sensitivity or were insulin-resistant or Type 2 diabetic. Resistin was not expressed in human muscle nor was it expressed in most human isolated fat cells or intact biopsies. No difference was found between normal, insulin-resistant, or Type 2 diabetic samples. However, a very low but specific Resistin expression could be demonstrated in isolated fat cells and intact adipose tissue from some individuals (n = 3 and n = 4, respectively). There was no evidence for the expression of splice variants in the human samples. Thus, Resistin does not seem to be an important link to insulin resistance and Type 2 diabetes in human.
authors.affiliation: Lundberg Laboratory for Diabetes Research, Department of Internal Medicine, Blå Stråket 5, Sahlgrenska University Hospital, S-413 45 Göteborg, Sweden. nagaev@medic.gu.se
authors.forename: I
authors.initials: I
authors.lastname: Nagaev
authors.forename: U
authors.initials: U
authors.lastname: Smith
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D017398:Alternative Splicing; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000235:genetics*; D014644:Genetic Variation; D006729:Hormones, Ectopic / Q000235:genetics*; D006801:Humans; D055504:Insulin Receptor Substrate Proteins; D007333:Insulin Resistance / Q000235:genetics*; D036341:Intercellular Signaling Peptides and Proteins; D007962:Leukocytes / Q000378:metabolism; D051379:Mice; D008875:Middle Aged; D018482:Muscle, Skeletal / Q000378:metabolism*; D020932:Nerve Growth Factor; D015345:Oligonucleotide Probes; D010750:Phosphoproteins / Q000235:genetics; D011506:Proteins; D052243:Resistin; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D006729:Hormones, Ectopic; C526292:IRS1 protein, human; D055504:Insulin Receptor Substrate Proteins; D036341:Intercellular Signaling Peptides and Proteins; C526295:Irs1 protein, mouse; D015345:Oligonucleotide Probes; D010750:Phosphoproteins; D011506:Proteins; C499624:RETN protein, human; C486574:RETNLB protein, human; D052243:Resistin; C499623:Retn protein, mouse; C413576:Retnla protein, mouse; D020932:Nerve Growth Factor