Cloning and functional characterization of the human heterogeneous nuclear ribonucleoprotein type I promoter.
We have cloned and functionally characterized a portion of the human hnRNP I (heterogeneous nuclear ribonucleoprotein type I) gene containing the promoter elements. HnRNP I is an alternative splicing modulator of tissue-specific transcripts that is expressed in three different isoforms. The DNA sequence at the transcription start site, identified by 5'-rapid amplification of cDNA ends, shows a high 'GC' content, lacks canonical TATA sequences and contains multiple putative Sp1 and NF1 transcription factor-binding sites, a GATA box and a CAAT box. By means of a chloramphenicol acetyltransferase reporter construct and deletion analyses, we have identified two regions between -770 bp and -206 bp that had a positive effect on expression activity in HeLa cells.
Romanelli
M G
MG
Department of Mother and Child, Biology and Genetics, University of Verona, Italy. romane@borgoroma.univr.it
Faggioli
L
L
Lorenzi
P
P
Morandi
C
C
D001483:Base Sequence; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D003001:Cloning, Molecular; D017930:Genes, Reporter; D006367:HeLa Cells; D034441:Heterogeneous-Nuclear Ribonucleoproteins; D006801:Humans; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012261:Ribonucleoproteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*
D034441:Heterogeneous-Nuclear Ribonucleoproteins; D012261:Ribonucleoproteins; D015500:Chloramphenicol O-Acetyltransferase