Determination of histamine in trypsine by capillary zone electrophoresis--a possible alternative to the bioassay?
A rapid capillary electrophoresis separation was developed in order to separate histamine from trypsine. A calibration curve with a determination limit of 1.0 microgram/ml histamine in 1 mg/ml trypsine was obtained. The method is suitable for the detection of small amounts of histamine contamination in trypsine with regard to the standards of the European Pharmacopoeia.
Miethe
G
G
Institute of Pharmacy, Humboldt University, Berlin, Germany. miethe@berlin.vci.de
Surmann
J P
JP
D001681:Biological Assay; D002138:Calibration; D019075:Electrophoresis, Capillary; D006632:Histamine / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet; D014357:Trypsin / Q000737:chemistry*
D006632:Histamine; D014357:Trypsin