title: Application of ion-impact energy measurement to electrospray ionization mass spectrometry of proteins and protein mixtures.
abstract: We have used a normal metal-insulator-superconductor (NIS) microcalorimeter to measure the impact energy of protein ions produced by electrospray ionization (ESI) in a magnetic-sector mass spectrometer (MS). We have used these measurements to resolve spectral ambiguities and to analyze protein mixtures. Energy measurement may be useful for the direct MS analysis of complex biopolymer mixtures that normally would confound ESI-MS deconvolution algorithms.
authors.affiliation: National Institute of Standards and Technology, Boulder, Colorado 80305-3328, USA. rabin@boulder.nist.gov
authors.forename: M W
authors.initials: MW
authors.lastname: Rabin
authors.forename: G C
authors.initials: GC
authors.lastname: Hilton
authors.forename: J M
authors.initials: JM
authors.lastname: Martinis
mesh_terms: D002151:Calorimetry; D004247:DNA / Q000032:analysis; D005934:Glucagon / Q000032:analysis; D007527:Isoenzymes / Q000032:analysis; D009113:Muramidase / Q000737:chemistry; D009211:Myoglobin / Q000032:analysis; D011506:Proteins / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D007527:Isoenzymes; D009211:Myoglobin; D011506:Proteins; D004247:DNA; D005934:Glucagon; D009113:Muramidase