Increased prostacyclin synthesis by atherosclerotic arteries from estrogen-treated monkeys.
We hypothesized that the atheroinhibitory and cardioprotective effects of estrogen may be mediated in part through increased prostacyclin formation by the artery wall. Atherosclerotic abdominal aorta was collected at necropsy from ovariectomized female monkeys fed an atherogenic diet alone or with added Premarin. Basal and arachidonate-stimulated prostacyclin and thromboxane synthesis by artery segments was measured by radioimmunoassay. In contrast to no observed differences in basal release of prostacyclin by the control and estrogen-treated arteries, there was a marked increase (approximately 165%) in arachidonate-stimulated formation of prostacyclin by estrogen-treated arteries, and prostacyclin synthesis was inversely correlated with plaque size. No differences were observed in basal or arachidonate-stimulated thromboxane synthesis by the control and estrogen-treated arteries. In light of known antiatherogenic and vasodilatory effects of estrogen, increased prostacyclin synthesis by estrogen-treated arteries may, in part, explain estrogen's beneficial effects on the artery wall.
O'Sullivan
M G
MG
Comparative Medicine Clinical Research Center, Wake Forest University School of Medicine, Winston-Salem, NC 27106-5193, USA. osull002@tc.umn.edu
Goodrich
J A
JA
Adams
M R
MR
D000818:Animals; D016718:Arachidonic Acid / Q000494:pharmacology; D001158:Arteries / Q000187:drug effects* / Q000378:metabolism* / Q000473:pathology; D001161:Arteriosclerosis / Q000378:metabolism*; D004036:Diet, Atherogenic; D004195:Disease Models, Animal; D011464:Epoprostenol / Q000378:metabolism*; D004966:Estrogens, Conjugated (USP) / Q000494:pharmacology*; D005260:Female; D066298:In Vitro Techniques; D008252:Macaca fascicularis; D010052:Ovariectomy; D011863:Radioimmunoassay; D013931:Thromboxanes / Q000378:metabolism
D004966:Estrogens, Conjugated (USP); D013931:Thromboxanes; D016718:Arachidonic Acid; D011464:Epoprostenol