title: Lipophilic quaternary ammonium salt acts as a mucosal adjuvant when co-administered by the nasal route with vaccine antigens.
abstract: Nasal administration of vaccines is an attractive approach which offers several significant advantages over traditional intramuscular vaccine delivery. These advantages include easier administration and induction of immune responses in the mucosal secretions of the body. In this study we describe a new potent nasal adjuvant, dimethyldioctadecylammonium bromide (DDA), that induces both mucosal and systemic immune responses when co-administered with diphtheria toxoid (DT), tetanus toxoid (TT) and BBG2Na antigens. In particular, we show that the nasal delivery of recombinant fragment (BBG2Na) of the G protein of respiratory syncytial virus (RSV) mixed with DDA induces both local and systemic anti-RSV immune responses and protects against viral challenge. Furthermore, we provide evidence that the DDA+BBG2Na vaccine does not induce lung immunopathology upon subsequent RSV challenge.
authors.affiliation: bio Mérieux-Pierre Fabre, Centre d'Immunologie Pierre Fabre, 5 Avenue Napoléon III, B.P. 497, St. Julien en Genevois, France.
authors.forename: C
authors.initials: C
authors.lastname: Klinguer
authors.forename: A
authors.initials: A
authors.lastname: Beck
authors.forename: P
authors.initials: P
authors.lastname: De-Lys
authors.forename: M C
authors.initials: MC
authors.lastname: Bussat
authors.forename: A
authors.initials: A
authors.lastname: Blaecke
authors.forename: F
authors.initials: F
authors.lastname: Derouet
authors.forename: J Y
authors.initials: JY
authors.lastname: Bonnefoy
authors.forename: T N
authors.initials: TN
authors.lastname: Nguyen
authors.forename: N
authors.initials: N
authors.lastname: Corvaïa
authors.forename: D
authors.initials: D
authors.lastname: Velin
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000281:Administration, Intranasal; D000818:Animals; D004168:Diphtheria Toxoid / Q000008:administration & dosage; D005260:Female; D018928:Immunity, Mucosal; D051379:Mice; D008807:Mice, Inbred BALB C; D000644:Quaternary Ammonium Compounds / Q000008:administration & dosage*; D012136:Respiratory Syncytial Viruses / Q000276:immunology*; D016205:Sigmodontinae; D013601:T-Lymphocytes / Q000276:immunology; D013745:Tetanus Toxoid / Q000008:administration & dosage; D014614:Vaccines, Synthetic / Q000008:administration & dosage*
chemical_list: D000276:Adjuvants, Immunologic; D004168:Diphtheria Toxoid; D000644:Quaternary Ammonium Compounds; D013745:Tetanus Toxoid; D014614:Vaccines, Synthetic; C015831:dimethyldioctadecylammonium