Thyroid stimulating hormone downregulates vascular endothelial growth factor expression in FRTL-5 cells.
We studied the regulation of the expression of vascular endothelial growth factor (VEGF) by TSH in monolayer cultures of rat thyroid FRTL-5 cell lines. The VEGF mRNA synthesis was significantly inhibited by TSH as well as dibutyryl cyclic adenosine monophosphate (cAMP) in a dose-dependent manner in FRTL-5 cells. This observation is contrary to previously published results using the thyroid follicular culture system. Our results suggest that the direct effect of TSH/cAMP is to inhibit the VEGF synthesis in monolayer cells.
Miyagi
E
E
Department of Pathology, Yamanashi Medical University, Nakakomagun, Japan.
Katoh
R
R
Li
X
X
Lu
S
S
Suzuki
K
K
Maeda
S
S
Shibuya
M
M
Kawaoi
A
A
D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002460:Cell Line; D015536:Down-Regulation; D016228:Endothelial Growth Factors / Q000235:genetics / Q000378:metabolism*; D008222:Lymphokines / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D013972:Thyrotropin / Q000494:pharmacology*; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
D016228:Endothelial Growth Factors; D008222:Lymphokines; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; D003994:Bucladesine; D013972:Thyrotropin