title: Oral administration of collagen conjugated with cholera toxin induces tolerance to type II collagen and suppresses chondritis in an animal model of autoimmune ear disease.
abstract: B10.RIII (H-2r) mice were orally administered cyanogen bromide peptide 11 (CB11) or cholera toxin B (CTB)-conjugated CB11 to induce tolerance in collagen-induced autoimmune ear disease. Oral administration of a high dosage of CB11 provided partial protection from chondritis. However, administration of a tiny amount of CTB-CB11 conjugate effectively suppressed chondritis. Oral administration of CTB-CB11 conjugate did not alter the stimulation of T cells in vitro or the fine specificities of B cells. The oral administration of CTB-CB11 caused a higher level of type II collagen-specific IgG and its subclass. Interestingly, increases of TH1 cytokine (interferon-gamma) in Peyer's patches and of TH1/TH2 cytokines (interleukin-2 and interleukin-4) in lymph nodes were detected in mice that had been fed CTB-CB11. An increase of CD8+ T cells in the Peyer's patches with a decrease of CD8+ T cells in lymph nodes was seen in mice that had been fed CTB-CB11. These results suggest that protection from chondritis by oral administration of minute amounts of CTB-CB11 conjugate can be achieved by a mechanism distinct from that of conventional oral tolerance induction.
authors.affiliation: Department of Medicine, University of Tennessee, Memphis 38163, USA.
authors.forename: N
authors.initials: N
authors.lastname: Kim
authors.forename: K C
authors.initials: KC
authors.lastname: Cheng
authors.forename: S S
authors.initials: SS
authors.lastname: Kwon
authors.forename: R
authors.initials: R
authors.lastname: Mora
authors.forename: M
authors.initials: M
authors.lastname: Barbieri
authors.forename: T J
authors.initials: TJ
authors.lastname: Yoo
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001327:Autoimmune Diseases / Q000276:immunology* / Q000473:pathology; D002772:Cholera Toxin / Q000494:pharmacology*; D003094:Collagen / Q000276:immunology*; D003488:Cyanogen Bromide / Q000494:pharmacology; D004427:Ear Diseases / Q000276:immunology* / Q000473:pathology; D007108:Immune Tolerance / Q000187:drug effects; D051379:Mice; D008815:Mice, Inbred Strains; D010007:Osteochondritis / Q000276:immunology* / Q000473:pathology; D010455:Peptides / Q000494:pharmacology
chemical_list: D010455:Peptides; D003094:Collagen; D002772:Cholera Toxin; D003488:Cyanogen Bromide