Density dependent interaction of polymeric analogs of beta-galactosyl ceramide with GP120 of human immunodeficiency virus 1.
Polymerizable analog of beta-galactosyl ceramide (beta-GalCer) was synthesized and converted to water-soluble polymers of beta-GalCer (poly-beta-GalCer) having specific affinity with recombinant gp120 of human immunodeficiency virus (HIV)-1 or V3 loop derived synthetic peptide. It was demonstrated that the binding affinity of poly-beta-GalCer greatly depends on the density of GalCer moieties on the polymer backbone.
Fujita
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Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo, Japan.
Nishimura
S I
SI
D000595:Amino Acid Sequence; D005699:Galactosylceramides / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism*; D015699:HIV Envelope Protein gp120 / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D015728:Receptors, HIV / Q000737:chemistry / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D005699:Galactosylceramides; D015699:HIV Envelope Protein gp120; C059762:HIV envelope protein gp120 (305-321); D010446:Peptide Fragments; D015728:Receptors, HIV; D011994:Recombinant Proteins