title: Protective anti-Helicobacter immunity is induced with aluminum hydroxide or complete Freund's adjuvant by systemic immunization.
abstract: To determine whether systemic immunization against Helicobacter pylori could be achieved with an adjuvant approved for human use, the efficacy of vaccination with Helicobacter antigen in combination with aluminum hydroxide (AlOH) was evaluated in a murine model of Helicobacter infection. Immunization with antigen and AlOH induced interleukin-5-secreting, antigen-specific T cells, and immunization with antigen and complete Freund's adjuvant induced interferon-gamma-secreting, antigen-specific T cells, as determined by ELISPOT assay. Both immune responses conferred protection after challenge with either H. pylori or H. felis, as confirmed by the complete absence of any bacteria, as assessed by both histology and culture of gastric biopsy samples. Protection was antibody independent, as demonstrated with antibody-deficient muMT mice (immunoglobulin-gene knockout mice), and CD4(+) spleen T cells from immunized mice were sufficient to transfer protective immunity to otherwise immunodeficient rag1(-/-) recipients. These results suggest an alternative and potentially more expeditious strategy for development of a human-use H. pylori vaccine.
authors.affiliation: Department of Pathology, Case Western Reserve University, Cleveland, Ohio, USA.
authors.forename: J M
authors.initials: JM
authors.lastname: Gottwein
authors.forename: T G
authors.initials: TG
authors.lastname: Blanchard
authors.forename: O S
authors.initials: OS
authors.lastname: Targoni
authors.forename: J C
authors.initials: JC
authors.lastname: Eisenberg
authors.forename: B M
authors.initials: BM
authors.lastname: Zagorski
authors.forename: R W
authors.initials: RW
authors.lastname: Redline
authors.forename: J G
authors.initials: JG
authors.lastname: Nedrud
authors.forename: M
authors.initials: M
authors.lastname: Tary-Lehmann
authors.forename: P V
authors.initials: PV
authors.lastname: Lehmann
authors.forename: S J
authors.initials: SJ
authors.lastname: Czinn
mesh_terms: D019264:Adoptive Transfer; D000536:Aluminum Hydroxide; D000818:Animals; D000942:Antigens, Bacterial / Q000008:administration & dosage / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D005620:Freund's Adjuvant; D005753:Gastric Mucosa / Q000473:pathology; D016998:Helicobacter / Q000276:immunology; D016481:Helicobacter Infections / Q000276:immunology* / Q000473:pathology / Q000517:prevention & control*; D016480:Helicobacter pylori / Q000276:immunology*; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D013601:T-Lymphocytes / Q000276:immunology*; D014611:Vaccination
chemical_list: D000942:Antigens, Bacterial; D000536:Aluminum Hydroxide; D005620:Freund's Adjuvant