cDNA sequence and mapping of the mouse Copb gene encoding the beta subunit of the COPI coatomer complex.
COPI-coated vesicles are involved in retrograde-directed selective transport of proteins from the Golgi complex to the endoplasmic reticulum (ER) as well as mediate anterograde transport of cargo proteins within the Golgi or in endosomal trafficking. The COPI protein complex contains an ADP-ribosylation factor (ARF1) and seven coatamer subunits (alpha, beta, beta', gamma, delta, epsilon, zeta-COP). The localization and function of human beta subunit of coatamer (COPB) suggests it is likely a candidate gene of ruby-eye-2 (ru2), which is a mouse model of human Hermansky-Pudlak syndrome characterized by the dysfunction of several subcellular organelles. In this study, we determined the entire coding sequence of mouse (Copb) cDNA by combining an overlapping mouse EST contig with EST walking. beta-COP was found highly conserved in mouse, rat, and human, and it is ubiquitously expressed in mouse. The Copb gene was mapped to mouse Chr 7 at a position of 53.3 cM by radiation hybrid mapping. Our RH mapping data, sequencing of RT-PCR products, and Western blotting exclude the Copb gene as a candidate for ru2.
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Department of Molecular and Cellular Biology, Roswell Park Cancer Institute, Elm and Carlton Sts, Buffalo, New York 14263, USA.
Elliott
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Novak
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EK
Swank
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RT
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016386:Chromosome Walking; D020755:Coat Protein Complex I / Q000235:genetics*; D020756:Coatomer Protein / Q000235:genetics*; D020451:Contig Mapping; D017931:DNA Primers; D051379:Mice; D008969:Molecular Sequence Data; D021101:Radiation Hybrid Mapping; D017422:Sequence Analysis, DNA
D020755:Coat Protein Complex I; D020756:Coatomer Protein; D017931:DNA Primers