title: [PCR-detection of markers for epidemiologically-significant strains of Vibrio cholerae 0139, isolated in Siberia].
abstract: Fourteen V. cholerae 0139 strains were isolated in 1996-1999 in Siberia from the Ob river (Novosibirsk) and bogs and lakes (Irkutsk). The strains were tested in PCR for the key virulence determinants (ctx AB, tcp, acf). The genomes lacked these elements, and therefore the strains were acknowledged avirulent. The results correlate completely with the data of phenotypical analysis, characterizing the pathogenic characteristics of isolated strains.
authors.affiliation: Plague Control Institute of Siberia and Far East, Irkutsk.
authors.forename: S V
authors.initials: SV
authors.lastname: Balakhonov
authors.forename: L V
authors.initials: LV
authors.lastname: Mironova
authors.forename: A S
authors.initials: AS
authors.lastname: Maramovich
authors.forename: V S
authors.initials: VS
authors.lastname: Ganin
authors.forename: L Ia
authors.initials: LIa
authors.lastname: Urbanovich
authors.forename: V I
authors.initials: VI
authors.lastname: Pogorelov
mesh_terms: D001483:Base Sequence; D002771:Cholera / Q000453:epidemiology* / Q000382:microbiology; D017931:DNA Primers; D005819:Genetic Markers; D010641:Phenotype; D016133:Polymerase Chain Reaction / Q000379:methods*; D012800:Siberia / Q000453:epidemiology; D014734:Vibrio cholerae / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D014774:Virulence / Q000235:genetics; D014871:Water Microbiology
chemical_list: D017931:DNA Primers; D005819:Genetic Markers