title: [Human Fab antibodies derived from phage display library neutralize hepatitis A virus in vitro].
abstract: OBJECTIVE
  Development of recombinant human monoclonal antibody to hepatitis A virus as a emergent measure for prevention of hepatitis A virus infection.
  
  
  METHODS
  Human neutralizing monoclonal antibody Fab fragments to HAV have been developed by using phage display technique. The heavy and light chains of human IgG Fab genes were amplified from a HAV patient in convalescent stage. The combinatorial phage antibody library was established by inserting both heavy and light chains of Fab genes into phage mid-vector pComb3 and followed by help phage infection after 4 rounds of panning with purified HAV as coated antigen.
  
  
  RESULTS
  The human Fab fragments to HAV were selected and expressed in bacteria.
  
  
  CONCLUSIONS
  The specific binding of Fab antibodies to HAV were demonstrated by their reaction with HAV antigen in ELISA. These results provide the basis for further development of a neutralizing recombinant human whole IgG molecule and hold promise for future use in the prophylaxis of HAV infection.
authors.affiliation: Institute of Virology, Chinese Academy of Preventive Medicine, Beijing 100052, China.
authors.forename: J
authors.initials: J
authors.lastname: Cao
authors.forename: M
authors.initials: M
authors.lastname: Liang
authors.forename: K
authors.initials: K
authors.lastname: Guo
mesh_terms: D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D001435:Bacteriophages / Q000235:genetics*; D017931:DNA Primers; D005784:Gene Amplification; D005803:Genes, Immunoglobulin / Q000235:genetics; D030041:Hepatitis A virus / Q000276:immunology*; D006508:Hepatitis Antibodies / Q000096:biosynthesis*; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000096:biosynthesis* / Q000235:genetics; D066298:In Vitro Techniques; D019151:Peptide Library
chemical_list: D000911:Antibodies, Monoclonal; D017931:DNA Primers; D006508:Hepatitis Antibodies; D007140:Immunoglobulin Fab Fragments; D019151:Peptide Library