The mRNA level of the potassium-chloride cotransporter KCC2 covaries with seizure susceptibility in inferior colliculus of the post-ischemic audiogenic seizure-prone rat.
Cardiac arrest and resuscitation were used to induce brain damage and susceptibility to sound-triggered seizures in Sprague-Dawley rats. Glucose preloading was used to vary seizure susceptibility. Because loop diuretics can block these seizures, we investigated changes in KCC2, a potassium-chloride cotransporter, in the inferior colliculus - the origin of the seizures. Using polymerase chain reaction (PCR), we found that collicular KCC2 mRNA levels covaried with seizure susceptibility in these animals. Using quantitative PCR, we found that a fivefold increase in collicular KCC2 mRNA levels was associated with a doubling of seizure incidence. A hypothesis linking KCC2 activity to seizure susceptibility is presented.
Reid
K H
KH
Department of Anatomical Sciences and Neurobiology, School of Medicine, University of Louisville, Louisville, KY, USA. khreid01@louisville.edu
Li
G Y
GY
Payne
R S
RS
Schurr
A
A
Cooper
N G
NG
D000818:Animals; D001786:Blood Glucose / Q000235:genetics; D002545:Brain Ischemia / Q000150:complications* / Q000378:metabolism / Q000503:physiopathology; D002352:Carrier Proteins / Q000235:genetics*; D018118:Chloride Channels / Q000235:genetics / Q000378:metabolism; D004195:Disease Models, Animal; D004198:Disease Susceptibility / Q000378:metabolism; D004232:Diuretics / Q000494:pharmacology; D004827:Epilepsy / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology; D020195:Epilepsy, Reflex / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology; D020022:Genetic Predisposition to Disease; D005947:Glucose / Q000494:pharmacology; D006324:Heart Arrest, Induced; D007245:Inferior Colliculi / Q000378:metabolism* / Q000503:physiopathology; D009474:Neurons / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D027981:Symporters; D015854:Up-Regulation / Q000235:genetics; D000096922:K Cl- Cotransporters
D001786:Blood Glucose; D002352:Carrier Proteins; D018118:Chloride Channels; D004232:Diuretics; D005947:Glucose; D012333:RNA, Messenger; D027981:Symporters; D000096922:K Cl- Cotransporters