Yb modulates the divisions of both germline and somatic stem cells through piwi- and hh-mediated mechanisms in the Drosophila ovary.
The coordinated division of distinctive types of stem cells within an organ is crucial for organogenesis and homeostasis. Here we show genetic interactions among fs(1)Yb (Yb), piwi, and hedgehog (hh) that regulate the division of both germline stem cells (GSCs) and somatic stem cells (SSCs), the two constituent stem cell populations of the Drosophila ovary. Yb is required for both GSC and SSC divisions; loss of Yb function eliminates GSCs and reduces SSC division, while Yb overexpression increases GSC number and causes SSC overproliferation. We also show that Yb acts via the piwi- and hh-mediated signaling pathways that emanate from the same signaling cells to control GSC and SSC division, respectively. hh signaling also has a minor effect in GSC division.
King
F J
FJ
Department of Cell Biology, Duke University Medical Center, Durham, North Carolina 27710, USA.
Szakmary
A
A
Cox
D N
DN
Lin
H
H
D000818:Animals; D060565:Argonaute Proteins; D002455:Cell Division; D002999:Clone Cells / Q000166:cytology / Q000378:metabolism; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000166:cytology* / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D018507:Gene Expression Regulation, Developmental; D005854:Germ Cells / Q000166:cytology* / Q000378:metabolism; D053823:Hedgehog Proteins; D007150:Immunohistochemistry; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D008954:Models, Biological; D009154:Mutation / Q000235:genetics; D010053:Ovary / Q000166:cytology* / Q000378:metabolism*; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D034743:RNA-Induced Silencing Complex; D011956:Receptors, Cell Surface; D015398:Signal Transduction; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism
D060565:Argonaute Proteins; D029721:Drosophila Proteins; D053823:Hedgehog Proteins; D019476:Insect Proteins; D008565:Membrane Proteins; D011506:Proteins; D034743:RNA-Induced Silencing Complex; D011956:Receptors, Cell Surface; C116498:piwi protein, Drosophila; C070844:ptc protein, Drosophila; C077245:hh protein, Drosophila