title: Colocalization of beta 1,4galactosyltransferase with mannose 6-phosphate receptor in monensin-induced TGN-derived structures.
abstract: Previously, we demonstrated that beta 1,4galactosyltransferase (gal-T1) reversibly segregates from alpha 2,6sialyltransferase (ST6Gal) to swollen vesicles after monensin treatment of the cells. To further explore this phenomenon, we investigated the response to monensin of various Golgi proteins. Within 30 min of monensin treatment, gal-T1 moved from the Golgi apparatus, as defined by localization of giantin, to swollen vesicles whereas ST6Gal, alpha 2,3(N)sialyltransferase, mannosidase II, and N-acetylgalactosaminyltransferase 2 remained associated with the Golgi apparatus. Stably transfected CHO cells exhibited a similar phenomenon of monensin-induced displacement of recombinant gal-T1 to swollen vesicles while recombinant ST6Gal remained colocalized with endogenously expressed giantin. Gal-T1 and the cation-insensitive mannose 6-phosphate receptor colocalized in swollen vesicles as observed at both light and electron microscopic levels. When monensin was replaced by chloroquine, gal-T1 remained arrested in swollen vesicles. Brefeldin A treatment known to cause relocation of Golgi-associated gal-T1 to the endoplasmic reticulum had no effect on gal-T1 trapped in swollen vesicles. This evidence suggests that monensin blocks gal-T1 trafficking in post-Golgi structures and argues against swelling of gal-T1-containing trans Golgi cisternae as previously assumed.
authors.affiliation: Institute of Physiology, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland. egberger@physiol.unizh.ch
authors.forename: E G
authors.initials: EG
authors.lastname: Berger
authors.forename: B
authors.initials: B
authors.lastname: Berger
authors.forename: M
authors.initials: M
authors.lastname: Höchli
authors.forename: A
authors.initials: A
authors.lastname: Dinter
mesh_terms: D000818:Animals; D016466:CHO Cells; D002478:Cells, Cultured; D006224:Cricetinae; D005347:Fibroblasts / Q000166:cytology; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D007476:Ionophores / Q000494:pharmacology; D016253:Microscopy, Immunoelectron; D008985:Monensin / Q000494:pharmacology; D009237:N-Acetyllactosamine Synthase / Q000032:analysis* / Q000235:genetics; D021381:Protein Transport / Q000187:drug effects; D017527:Receptor, IGF Type 2 / Q000032:analysis*; D014162:Transfection; D021601:trans-Golgi Network / Q000737:chemistry* / Q000201:enzymology* / Q000648:ultrastructure
chemical_list: D007476:Ionophores; D017527:Receptor, IGF Type 2; D008985:Monensin; D009237:N-Acetyllactosamine Synthase