title: Evaluating pediatric brain tumor cellularity with diffusion-tensor imaging.
abstract: OBJECTIVE
  MR imaging of central nervous system (CNS) malignancies falls short of a definitive evaluation. Tissue diagnosis remains the gold standard. Diffusion-tensor MR imaging measures the apparent diffusion coefficient and diffusion anisotropy of water in tissue. The purpose of this study was to test the hypothesis that the apparent diffusion coefficient may improve the MR imaging evaluation of newly diagnosed CNS neoplasms. We examined the relationship between the apparent diffusion coefficient, anisotropy, and tumor cellularity in 12 pediatric patients.
  
  
  METHODS
  On the basis of histopathologic evaluation, tumors in this case series were segregated into three types: low-grade gliomas, embryonal tumors, and nonembryonal high-grade tumors. Mean apparent diffusion coefficient and anisotropy values obtained from the solid components of each tumor were compared with cellularity, total cellular area, and total nuclear area derived from biopsy material.
  
  
  RESULTS
  The apparent diffusion coefficient ratio (tumor to normal brain) correlated well with tumor classification (p = 0.001). Anisotropy was decreased similarly in all tumor classifications. The absolute apparent diffusion coefficient correlated well with cellularity (p = 0.014) and total nuclear area (p = 0.005) per high-power field. The correlation between apparent diffusion coefficient and total cellular area per high-power field was not statistically significant.
  
  
  CONCLUSIONS
  The apparent diffusion coefficient may be predictive of tumor classification and may be a useful tool in characterizing tumor cellularity and total nuclear area. These parameters are not available in standard MR imaging. Therefore, diffusion-tensor imaging may enhance the diagnostic process in pediatric CNS malignancies.
authors.affiliation: Department of Pediatrics, St. Louis Children's Hospital, Rm. 3S34, One Children's PI., St. Louis, MO 63110, USA.
authors.forename: K M
authors.initials: KM
authors.lastname: Gauvain
authors.forename: R C
authors.initials: RC
authors.lastname: McKinstry
authors.forename: P
authors.initials: P
authors.lastname: Mukherjee
authors.forename: A
authors.initials: A
authors.lastname: Perry
authors.forename: J J
authors.initials: JJ
authors.lastname: Neil
authors.forename: B A
authors.initials: BA
authors.lastname: Kaufman
authors.forename: R J
authors.initials: RJ
authors.lastname: Hayashi
mesh_terms: D000293:Adolescent; D016880:Anisotropy; D001254:Astrocytoma / Q000473:pathology; D001921:Brain / Q000473:pathology*; D001932:Brain Neoplasms / Q000473:pathology*; D002648:Child; D002675:Child, Preschool; D005260:Female; D005910:Glioma / Q000473:pathology; D006801:Humans; D007223:Infant; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D009373:Neoplasms, Germ Cell and Embryonal / Q000473:pathology; D012189:Retrospective Studies