Effect of ursodeoxycholate in University of Wisconsin solution on long term preservation of rat livers.
OBJECTIVE
To find out whether the addition of ursodeoxycholate (UDC) to University of Wisconsin (UW) solution reduces injury during preservation of the liver.


METHODS
Controlled laboratory study.


METHODS
University hospital, Germany.


METHODS
Preserved livers from 12 rats.


METHODS
Livers were flushed with 10 ml of ice-cold (0-1 degrees C) UW or iso-osmolar UW containing 5 mmol/l UDC. After preservation for 30 hours at 0-1 degrees C and 1 hour at 22 degrees C the livers were reperfused for 4 hours. Aspartate amino-transaminase, alanine aminotransferase, glutamate lactate dehydrogenase, and white blood cell (WBC) and platelet counts were measured during reperfusion. Bile flow and total bile salt secretion rate were also measured.


RESULTS
Addition of UDC to UW reduced the release of liver enzymes, slightly increased platelet adherence, and reduced bile flow and bile salt secretion rate.


CONCLUSIONS
The membrane-stabilising effect of UDC may explain the reduction in enzyme release. However, liver function, as expressed by bile salt secretion rate and bile flow was adversely affected.
Hertl
M
M
Department of General and Transplantation Surgery, University Hospital, Essen, Germany.
Clausnitzer
N
N
Hertl
M C
MC
Broelsch
C E
CE
D000241:Adenosine / Q000737:chemistry*; D000493:Allopurinol / Q000737:chemistry*; D000818:Animals; D005978:Glutathione / Q000737:chemistry*; D066298:In Vitro Techniques; D007328:Insulin / Q000737:chemistry*; D008099:Liver; D008111:Liver Function Tests; D008297:Male; D023421:Models, Animal; D009926:Organ Preservation; D019188:Organ Preservation Solutions / Q000737:chemistry*; D011887:Raffinose / Q000737:chemistry*; D051381:Rats; D011917:Rats, Inbred Lew; D014580:Ursodeoxycholic Acid
D007328:Insulin; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; D014580:Ursodeoxycholic Acid; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose