Superoxide dismutase, glutathione peroxidase activities and the hydroperoxide concentration are modified in the hippocampus of epileptic rats.
The relationship between free radical and scavenger enzymes has been found in the epileptic phenomena and reactive oxygen species have been implicated in seizure-induced neurodegeneration. Using the epilepsy model obtained by systemic administration of pilocarpine (PILO) in rats, we investigated the superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities as well as the hydroperoxide (HPx) concentration in the hippocampus of rats during status epilepticus (SE), silent and chronic periods. The enzyme activities as well as the HPx concentration were measured using spectrophotometric methods and the results compared to values obtained from saline-treated animals. The SOD activity decreased after long-lasting SE period and during the chronic phase. In addition, HPx levels increased in same periods whereas the GPx activity increased only in the hippocampus of animals submitted to 1 h of SE. Animals presenting partial seizures, those submitted to 5 h of SE and animals from the silent period (seizure free) showed normal levels of SOD, GPx and HPx. These results show a direct evidence of lipid peroxidation during seizure activity that could be responsible for neuronal damage in the hippocampus of rats, during the establishment of PILO model of epilepsy.
Bellissimo
M I
MI
Disciplinas de Neurologia Experimental, Escola Paulista de Medicina, Universidade Federal de São Paulo (UNIFESP), Rua Botucatu 862, 04023-900 Sao Paulo, Brazil.
Amado
D
D
Abdalla
D S
DS
Ferreira
E C
EC
Cavalheiro
E A
EA
Naffah-Mazzacoratti
M G
MG
D000818:Animals; D002908:Chronic Disease; D004827:Epilepsy / Q000139:chemically induced / Q000378:metabolism*; D005979:Glutathione Peroxidase / Q000378:metabolism*; D006624:Hippocampus / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000378:metabolism*; D008297:Male; D018721:Muscarinic Agonists; D009994:Osmolar Concentration; D010862:Pilocarpine; D051381:Rats; D017208:Rats, Wistar; D013226:Status Epilepticus / Q000139:chemically induced / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism*
D018721:Muscarinic Agonists; D010862:Pilocarpine; D006861:Hydrogen Peroxide; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase