Toxicity of sediments from Bahía de Chetumal, México, as assessed by hepatic EROD induction and histology in nile tilapia Oreochromis niloticus.
The effect of environmental pollutants present in sediments obtained from Bahía de Chetumal, a bay on the border between Mexico and Belize, was studied in nile tilapia (Oreochromis niloticus) intraperitoneally injected with sediment extracts from six different sites of the Bay. Sediment samples used for the study contained a variety of organic chemicals such as organochlorine pesticides, polychlorinated biphenyls (PCBs) and polynuclear aromatic hydrocarbons (PAHs). Total cytochrome P-450 and EROD activity were measured in fish liver. Haematological and histological analyses were also carried out. Hepatic P-450 content in treated fish increased from 43 to 240%, and EROD activity from 85 to 160% compared to controls. Extracts from two sampling sites inhibited EROD activity. There were positive significant correlations between P-450 content and the levels of PCBs 44 and 128. EROD activity correlated to HCB, op'-DDE, pp'-DDE, pp'-DDD, mirex and PCB 18 concentrations. Blood examination showed cell degeneration and binucleated leukocytes with abnormal chromatin. Extract treatment also resulted in foci of hyperplasia on the basement of gill lamellae, hypertrophy and oedema in gills and liver necrosis. Control fish showed no abnormalities. The results demonstrate that sediments from Bahía of Chetumal have the potential to cause histopathological, haematological and biochemical alterations in fish. The administration of sediment extracts to fish may serve as a useful test to screen the toxicity of sediments from different areas.
Zapata-Pérez
O
O
Cinvestav Unidad Mérida, Depto de Recursos del Mar, Apdo. Postal. 73, C.P 97310 Mérida, Yucatán, Mexico.
Simá-Alvarez
R
R
Noreña-Barroso
E
E
Güemes
J
J
Gold-Bouchot
G
G
Ortega
A
A
Albores-Medina
A
A
D000818:Animals; D019363:Cytochrome P-450 CYP1A1 / Q000096:biosynthesis*; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis; D003633:Dichlorodiphenyl Dichloroethylene / Q000032:analysis; D003632:Dichlorodiphenyldichloroethane / Q000032:analysis; D004790:Enzyme Induction; D019015:Geologic Sediments / Q000737:chemistry*; D006581:Hexachlorobenzene / Q000032:analysis; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D008800:Mexico; D012989:Soil Pollutants / Q000633:toxicity*; D017210:Tilapia / Q000378:metabolism*
D012989:Soil Pollutants; D003633:Dichlorodiphenyl Dichloroethylene; D006581:Hexachlorobenzene; D003577:Cytochrome P-450 Enzyme System; D019363:Cytochrome P-450 CYP1A1; D003632:Dichlorodiphenyldichloroethane