title: Backtable heat-enhanced preconditioning: a simple and effective means of improving function of heart transplants.
abstract: BACKGROUND
  Cardiac harvest teams are usually committed to immediately transfer the explanted donor heart into its cold storage solution. We tested the opposite hypothesis that a brief prestorage episode of heat-enhanced ischemic preconditioning could be protective.
  
  
  METHODS
  Fifty-three isolated isovolumic rat hearts underwent 4 hours of cold (4 degrees C) storage in the Celsior preservation solution and 2 hours of reperfusion. Control hearts were immediately immersed after arrest. In the 3 treated groups, 2 customized thermal probes were first applied onto the left ventricular free wall of the explanted heart at 22 degrees C, 37 degrees C or 42.5 degrees C for 15 minutes before immersion. Each of the selected temperatures were monitored at the probe-tissue interface by a thermocouple.
  
  
  RESULTS
  Whereas base line end-diastolic pressure was set at = 8 mm Hg in all groups, it increased during reperfusion (mean +/- SEM) to 28+/-3, 27+/-3, 17+/-1, and 18+/-2 mm Hg in control, 22 degrees C, 37 degrees C and 42.5 degrees C-heated hearts, respectively (37 degrees C and 42.5 degrees C: p < 0.05 versus controls and 22 degrees C). Slopes of pressure-volume curves featured similar patterns. Likewise, reperfusion dP/dT (mm Hg/s(-1)) was significantly lower in control and 22 degrees C hearts (1,119+/-114 and 1,076+/-125, respectively) than in those undergoing prestorage heating to 37 degrees C and 42.5 degrees C (1,545+/-109 and 1,719+/-111, p < 0.05 and p < 0.01 versus controls and 22 degrees C, respectively). Western blot analysis of LV samples did not demonstrate any upregulation of HSP 72 in either group. Conversely, the involvement of preconditioning was evidenced by the loss of protection in the 42.5 degrees C-heated hearts when, in 2 additional groups, the storage solution was supplemented with either the protein kinase C and tyrosine kinase inhibitors chelerythrine (5 micromol/L) and genistein (50 micromol/L) or the mitochondrial K(ATP) channel inhibitor 5-hydroxydecanoate (200 micromol/L).
  
  
  CONCLUSIONS
  A brief period of postexplant ischemia with enhancement by topical heating ("backtable preconditioning") could be a simple and effective means of improving the functional recovery of heart transplants.
authors.affiliation: Department of Cardiovascular Surgery, Hĵpital Bichat, Paris, France.
authors.forename: E
authors.initials: E
authors.lastname: Kevelaitis
authors.forename: A P
authors.initials: AP
authors.lastname: Patel
authors.forename: A
authors.initials: A
authors.lastname: Oubenaissa
authors.forename: J
authors.initials: J
authors.lastname: Peynet
authors.forename: C
authors.initials: C
authors.lastname: Mouas
authors.forename: D M
authors.initials: DM
authors.lastname: Yellon
authors.forename: P
authors.initials: P
authors.lastname: Menasché
mesh_terms: D000818:Animals; D003971:Diastole / Q000502:physiology; D050884:HSP72 Heat-Shock Proteins; D016027:Heart Transplantation; D006360:Heat-Shock Proteins / Q000378:metabolism; D006361:Heating; D019194:Ischemic Preconditioning; D008297:Male; D009200:Myocardial Contraction / Q000502:physiology*; D009926:Organ Preservation; D051381:Rats; D017208:Rats, Wistar; D013599:Systole / Q000502:physiology; D016277:Ventricular Function, Left / Q000502:physiology
chemical_list: D050884:HSP72 Heat-Shock Proteins; D006360:Heat-Shock Proteins