Conjugation of Arg-Gly-Asp (RGD) sequence in copolymer bearing sugar moiety for insulinoma cell line (MIN6) culture.
Copolymers composed of an Arg-Gly-Asp (RGD) sequence for the adhesion molecule and sugar moieties were synthesized for an insulinoma cell (MIN6) culture. MIN6 cells attached on the poly(N-p-vinylbenzyl-D-maltonamide-co-6-(p-vinylbenzamido)-hexanoic acid-g-GRGDS) (p(VMA-co-VBGRGDS))-coated dishes were in a more aggregated form than other polymer-coated surfaces. P(VMA-co-VBGRGDS) also shows faster proliferation of MIN6 cells (about 18% higher) than with p(VLA-co-VBGRGDS). By interaction between cell and matrix, about 80% greater insulin secretion from MIN6 cells was produced with the p(VMA-co-VBGRGDS), and about 50% greater insulin secretion was produced with the poly(N-p-vinylbenzyl-D-lactonamide-co-6-(p-vinylbenzamido)-hexanoic acid-g-GRGDS) (p(VLA-co-VBGRGDS) as compared with unstimulated cells. Moreover, attachment of MIN6 cells treated with RGD monomer was suppressed approximately 50% for the p(VMA-co-VBGRGDS) surface. This result supported the idea that conjugation of adhesion molecules of RGD peptide in p(VMA-co-VBGRGDS) copolymer specifically interact with integrin families on MIN6 cell membrane.
Na
K
K
College of Pharmacy, Chosun University, Kwangju, Korea.
Choi
H K
HK
Akaike
T
T
Park
K H
KH
D002240:Carbohydrate Sequence; D002448:Cell Adhesion; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007340:Insulinoma / Q000378:metabolism*; D008320:Maltose / Q000138:chemical synthesis; D009842:Oligopeptides / Q000138:chemical synthesis / Q000378:metabolism*; D010190:Pancreatic Neoplasms / Q000378:metabolism*; D011137:Polystyrenes / Q000138:chemical synthesis; D014407:Tumor Cells, Cultured
D009842:Oligopeptides; D011137:Polystyrenes; C423202:poly(N-p-vinylbenzyl-maltonamide); D008320:Maltose; C047981:arginyl-glycyl-aspartic acid