[Isolation and identification of PACE-binding protein rpn4--a new transcription activator, participating in regulation of 26S proteosome and other genes].
A new upstream activating sequence (UAS) 5'-GGTGGCAAA-3' was detected in the promoters of 27 out of the 33 proteasomal genes and of several genes related to the ubiquitin-proteasomal system of Saccharomices cerevisiae. The sequence was termed proteasome-associated control element (PACE). Gel retardation assay revealed specific binding of PACE with an extracted protein. The protein (64K) was purified by affinity chromatography and was homogeneous by SDS-PAGE. Microsequencing showed that the protein is Rpn4p. The ability of Rpn4p to activate transcription was demonstrated with constructs containing fragments of the RPN5 and CDC48 gene promoters and reporter cat. Binding to PACE, Rpn4p may act as a common transcription factor on the proteasomal and proteasome-related genes.
Kapranov
A B
AB
Kuriatova
M V
MV
Preobrazhenskaia
O V
OV
Tiutiaeva
V V
VV
Shtuka
R
R
Feldmann
H
H
Karpov
V L
VL
D002413:Cations, Divalent; D002846:Chromatography, Affinity; D003546:Cysteine Endopeptidases / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000302:isolation & purification* / Q000378:metabolism / Q000502:physiology; D005786:Gene Expression Regulation / Q000502:physiology*; D005800:Genes, Fungal; D009097:Multienzyme Complexes / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D046988:Proteasome Endopeptidase Complex; D012441:Saccharomyces cerevisiae / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins; D015534:Trans-Activators / Q000302:isolation & purification* / Q000378:metabolism / Q000502:physiology; D014157:Transcription Factors / Q000302:isolation & purification* / Q000378:metabolism / Q000502:physiology
D002413:Cations, Divalent; D004268:DNA-Binding Proteins; D009097:Multienzyme Complexes; C119430:RPN4 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D015534:Trans-Activators; D014157:Transcription Factors; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex