Electrophoretic behavior of peptides in capillary electrophoresis influence of ionic strength and pH in aqueous-organic media.
Through correct pH, pKa and activity coefficients values, a model describing the effect of pH on electrophoretic mobility of substances has been applied to a series of peptides in water and in acetonitrile-water mixtures. The derived equations permit prediction of the optimum pH for the electrophoretic separation from only a few experimental values and they also permit determination of pKa values of analytes in the aqueous-organic media employed. Furthermore, the electrophoretic resolution between pairs of substances can be predicted, in order to evaluate electrophoretic separations of the studied peptides.
Sanz-Nebot
V
V
Department of Analytical Chemistry, University of Barcelona, Spain. vsanz@apolo.qui.ub.es
Benavente
F
F
Toro
I
I
Barbosa
J
J
D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D009930:Organic Chemicals; D009994:Osmolar Concentration; D010455:Peptides / Q000737:chemistry*; D012997:Solvents; D014867:Water
D009930:Organic Chemicals; D010455:Peptides; D012997:Solvents; D014867:Water