Copper staining method for extracting biologically active proteins from native gels.
An attempt was made to make protein bands visible on native gel using copper staining, since such a mild staining procedure would make the entire native gel electrophoresis process non-denaturing. Copper staining not only was able to detect various proteins on native gel with reasonable sensitivity, but also made extraction and recovery of active proteins possible from the gel using a gentle procedure.
Wen
J
J
Amgen, Inc., Amgen Center, Thousand Oaks, CA 91320, USA.
Zhang
M
M
Horan
T P
TP
Philo
J S
JS
Li
T
T
Wypych
J
J
Mendiaz
E A
EA
Langley
K E
KE
Aoki
K H
KH
Kuwamoto
M
M
Kita
Y
Y
Arakawa
T
T
D002850:Chromatography, Gel; D003300:Copper / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D005782:Gels / Q000737:chemistry; D011506:Proteins / Q000302:isolation & purification*; D013194:Staining and Labeling
D005782:Gels; D011506:Proteins; D003300:Copper