Molecular cloning and characterization of a novel SH3 protein (SLY) preferentially expressed in lymphoid cells.
A novel full-length cDNA was isolated from a murine T-cell lymphoma library that has an open reading frame encoding 381 amino acids. The predicted protein (termed SLY) contains a Src homology 3 domain and a sterile alpha motif, suggesting that it functions as a signaling adaptor protein in lymphocytes. Northern blot and in situ hybridization analysis showed a preferential expression in lymphoid tissues. The sly gene is located on the X-chromosome in close proximity to genes involved in various immune disorders. This is consistent with an additional role of SLY in immune pathology.
Beer
S
S
Institute of Medical Microbiology, immunology and Hygiene, Technische Universität München, Munich, Germany. sbeer@lrz.tu-muenchen.de
Simins
A B
AB
Schuster
A
A
Holzmann
B
B
D000818:Animals; D002352:Carrier Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D002460:Cell Line; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000302:isolation & purification; D015723:Gene Library; D017874:Immediate-Early Proteins; D017403:In Situ Hybridization; D008221:Lymphoid Tissue / Q000378:metabolism; D016399:Lymphoma, T-Cell / Q000235:genetics / Q000378:metabolism; D051379:Mice; D051938:Munc18 Proteins; D012333:RNA, Messenger / Q000096:biosynthesis; D015398:Signal Transduction; D014162:Transfection; D018909:src Homology Domains
D002352:Carrier Proteins; D018076:DNA, Complementary; D017874:Immediate-Early Proteins; D051938:Munc18 Proteins; D012333:RNA, Messenger; C106568:Scfd1 protein, rat