title: Transposition of IS1397 in the family Enterobacteriaceae and first characterization of ISKpn1, a new insertion sequence associated with Klebsiella pneumoniae palindromic units.
abstract: IS1397 and ISKpn1 are IS3 family members which are specifically inserted into the loop of palindromic units (PUs). IS1397 is shown to transpose into PUs with sequences close or identical to the Escherichia coli consensus, even in other enterobacteria (Salmonella enterica serovar Typhimurium, Klebsiella pneumoniae, and Klebsiella oxytoca). Moreover, we show that homologous intergenic regions containing PUs constitute IS1397 transpositional hot spots, despite bacterial interspersed mosaic element structures that differ among the three species. ISKpn1, described here for the first time, is specific for PUs from K. pneumoniae, in which we discovered it. A sequence comparison between the two insertion sequences allowed us to define a motif possibly accounting for their specificity.
authors.affiliation: Unité de Programmation Moléculaire et Toxicologie Génétique, CNRS URA 1444, Institut Pasteur, 75724 Paris Cedex 15, France.
authors.forename: C
authors.initials: C
authors.lastname: Wilde
authors.forename: S
authors.initials: S
authors.lastname: Bachellier
authors.forename: M
authors.initials: M
authors.lastname: Hofnung
authors.forename: J M
authors.initials: JM
authors.lastname: Clément
mesh_terms: D001483:Base Sequence; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D004755:Enterobacteriaceae / Q000235:genetics*; D007711:Klebsiella pneumoniae / Q000235:genetics*; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic
chemical_list: D004251:DNA Transposable Elements; D004269:DNA, Bacterial