title: Evaluation of fumonisin-aflatoxin co-occurrence in Brazilian corn hybrids by ELISA.
abstract: The natural co-occurrence of fumonisins and aflatoxins was investigated in freshly harvested corn kernels (150 samples, 62 hybrids), acquired from the Central-Southern (27 samples, 21 hybrids), Central-Western (86 samples, 51 hybrids) and Northern (37 samples, 18 hybrids) regions of the State of Paraná, Brazil using enzyme-linked immunosorbent assay (ELISA). Fumonisins were detected in 147 (98%) samples at a concentration range of 0.096 to 22.6 microg/g, while aflatoxins were detected in 17 (11.3%). All the aflatoxin-positive samples (range 38.0-460.0 ng/g) came from the Central-Western region and were co-contaminated with fumonisins. Fumonisin contamination was higher in corn from the Northern (9.85 microg/g) and Central-Western regions (5.08 microg/g), when compared with the Central-Southern region (1.14 microg/g). The overall evaluation detected 62% samples with fumonisin levels < or = 5.0 microg/g. Regional differences affected fumonisin levels in the same hybrid, regardless of Fusarium count and moisture content, suggesting interference from climatic conditions, in addition to the local predominance of toxigenic strains of the Fusarium biotype.
authors.affiliation: Department of Biochemistry-CCE, State University of Londrina, Paraná, Brazil. eysono@uel.br
authors.forename: E Y
authors.initials: EY
authors.lastname: Ono
authors.forename: M A
authors.initials: MA
authors.lastname: Ono
authors.forename: F Y
authors.initials: FY
authors.lastname: Funo
authors.forename: A E
authors.initials: AE
authors.lastname: Medinal
authors.forename: T C
authors.initials: TC
authors.lastname: Oliveira
authors.forename: O
authors.initials: O
authors.lastname: Kawamura
authors.forename: Y
authors.initials: Y
authors.lastname: Ueno
authors.forename: E Y
authors.initials: EY
authors.lastname: Hirooka
mesh_terms: D000348:Aflatoxins / Q000032:analysis*; D000818:Animals; D000911:Antibodies, Monoclonal; D001938:Brazil; D002264:Carboxylic Acids / Q000032:analysis*; D002980:Climate; D003429:Cross Reactions; D002523:Edible Grain / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D037341:Fumonisins
chemical_list: D000348:Aflatoxins; D000911:Antibodies, Monoclonal; D002264:Carboxylic Acids; D037341:Fumonisins; C056934:fumonisin B2; C073063:fumonisin B3; C056933:fumonisin B1