Increased levels of comet-detected spermatozoa DNA damage following in vivo isotopic- or X-irradiation of spermatogonia.
To investigate whether DNA damage arising in spermatogenic germ cells can be detected in resultant sperm, we have irradiated murine testis and collected spermatozoa from the vas deferens 45 days later. These cells were derived from spermatogonia present at the time of irradiation. Two forms of irradiation were used, external X-rays (4Gy) and internal auger electrons from contamination of the male mouse with the isotope Indium-114m (1.85MBq), which was localised in the testis. Both forms of irradiation produced a profound fall in vas deferens sperm count and testis weight, Indium-114m being more effective. Using the neutral Comet assay for double strand break detection, significant increases in sperm comet tail length and moment were observed. The levels of damage were similar for both treatments. Care had to be taken during the assay to distinguish between sperm and somatic cells as the proportion of the latter increased after irradiation. We conclude that the comet assay can detect DNA damage in spermatozoa after the in vivo exposure of male germ cells to a known testicular genotoxic agent. The assay may be useful for the assessment of sperm DNA damage (double stranded) associated with male infertility and post-fertilization developmental abnormalities in the offspring.
Haines
G A
GA
School of Biological Sciences, University of Manchester, Manchester, UK.
Hendry
J H
JH
Daniel
C P
CP
Morris
I D
ID
D000818:Animals; D020552:Comet Assay; D004247:DNA / Q000528:radiation effects*; D004249:DNA Damage; D007205:Indium Radioisotopes / Q000493:pharmacokinetics / Q000633:toxicity*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D013093:Spermatogonia / Q000528:radiation effects*; D014965:X-Rays
D007205:Indium Radioisotopes; D004247:DNA