In situ hybridization of somatolactin transcripts in the pituitary glands from acclimatized carp (Cyprinus carpio).
We isolated and cloned a carp somatolactin SL DNA fragment, of which 78% of the nucleotides were identical to the corresponding salmon SL sequence. The results obtained upon Northern blot hybridization of carp pituitary RNA allowed the identification of two transcripts as described for other fish. When the content of SL transcripts in pituitary sections from summer- and winter-acclimatized carp was quantified by in situ hybridization assays, we found no significant differences between the two seasons. In salmonids, plasma SL reaches higher levels in summer than in winter in synchrony with the water temperature cycle; in the eurythermal carp, however, the complex adaptive responses imposed by seasonal environmental changes do not seem to include the regulation of the somatolactin detected with the probe used at the transcriptional level in pituitary glands.
López
M
M
Facultad de Ciencias de la Salud, Universidad Nacional Andrés Bello and MIFAB, Santiago, Chile.
Figueroa
J
J
Kausel
G
G
Vera
M I
MI
Krauskopf
M
M
D000064:Acclimatization / Q000502:physiology; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002347:Carps / Q000502:physiology*; D053938:DNA Fragmentation; D029941:Fish Proteins; D006023:Glycoproteins / Q000235:genetics* / Q000302:isolation & purification; D017403:In Situ Hybridization / Q000379:methods*; D010902:Pituitary Gland / Q000502:physiology*; D010907:Pituitary Hormones / Q000235:genetics* / Q000302:isolation & purification; D012474:Salmon; D012621:Seasons; D014158:Transcription, Genetic
D029941:Fish Proteins; D006023:Glycoproteins; D010907:Pituitary Hormones; C064448:somatolactin protein, fish