Inhibitory effects of T-cell stimulation and co-stimulation observed at high concentrations of plate-bound antibodies.
Plate-bound monoclonal antibodies (mAb) are often used as a way of stimulating lymphocytes in vitro. Our observations show that the concentrations of mAb used in functional assays in vitro must be carefully assessed before conclusions are drawn about lymphocyte activation or co-activation.
Lesage
S
S
PROCREA BioSciences Inc., Division of Research and Development, 6100 Royalmount, H4P 2R2, Montreal, Quebec, Canada.
Charron
J
J
Hugo
P
P
D000906:Antibodies / Q000494:pharmacology*; D018106:CD28 Antigens / Q000276:immunology; D008213:Lymphocyte Activation / Q000187:drug effects / Q000276:immunology*; D011948:Receptors, Antigen, T-Cell / Q000276:immunology; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology*
D000906:Antibodies; D018106:CD28 Antigens; D011948:Receptors, Antigen, T-Cell