An improved fixation technique for fluorescence in situ hybridization for preimplantation genetic diagnosis.
OBJECTIVE
To improve existing preimplantation genetic diagnosis fixation techniques.


METHODS
Prospective randomized in vitro study.


METHODS
Academic medical center.


METHODS
None.


METHODS
None.


METHODS
The intensity and clarity of fluorescence in situ hybridization (FISH) signals and the percentage of successfully fixed blastomeres.


RESULTS
The described fixation technique resulted in 100% fixation and 100% adequate FISH signals. Two conventional techniques resulted in 94% and 87% fixation, and in adequate FISH signals in 81% and 87%, respectively.


CONCLUSIONS
This newly developed fixation technique simplifies the process of fixation of blastomeres for preimplantation diagnosis while essentially eliminating the possibility of losing a cell during fixation. It will hopefully allow more IVF programs to offer their patients preimplantation genetic diagnosis using the FISH technique.
Dozortsev
D I
DI
Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Hutzel Hospital, Wayne State University, Detroit, MI 48201, USA. aa4421@wayne.edu
McGinnis
K T
KT
D001757:Blastomeres; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D019836:Preimplantation Diagnosis / Q000379:methods*; D011446:Prospective Studies; D011897:Random Allocation; D016707:Tissue Fixation / Q000379:methods*