Chloride channel antagonists perturb growth and morphology of Neurospora crassa.
The chloride channel antagonists anthracene-9-carboxylic acid, ethacrynic acid and niflumic acid were found to be fungistatic and morphogenic when tested against the ascomycete Neurospora crassa. Potency increased with decreasing pH, suggesting that the protonated forms of the compounds were active. Niflumic acid produced the most pronounced growth aberrations which may reflect an ability to acidify the cytoplasm and block the plasma membrane anion channel of N. crassa.
Hanke
G T
GT
Department of Plant Sciences, University of Cambridge, Downing Street, CB2 3EA, Cambridge, UK.
Northrop
F D
FD
Devine
G R
GR
Bothwell
J H
JH
Davies
J M
JM
D000362:Agar; D000873:Anthracenes / Q000494:pharmacology*; D000935:Antifungal Agents / Q000494:pharmacology; D018533:Biomass; D002455:Cell Division / Q000187:drug effects; D018118:Chloride Channels / Q000037:antagonists & inhibitors*; D003470:Culture Media; D004976:Ethacrynic Acid / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D009492:Neurospora crassa / Q000166:cytology* / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D009544:Niflumic Acid / Q000494:pharmacology*
D000873:Anthracenes; D000935:Antifungal Agents; D018118:Chloride Channels; D003470:Culture Media; D009544:Niflumic Acid; C010030:9-anthroic acid; D000362:Agar; D004976:Ethacrynic Acid