Chemical genetic approaches for the elucidation of signaling pathways.
New chemical methods that use small molecules to perturb cellular function in ways analogous to genetics have recently been developed. These approaches include both synthetic methods for discovering small molecules capable of acting like genetic mutations, and techniques that combine the advantages of genetics and chemistry to optimize the potency and specificity of small-molecule inhibitors. Both approaches have been used to study protein function in vivo and have provided insights into complex signaling cascades.
Alaimo
P J
PJ
Department of Cellular and Molecular Pharmacology, University of California, San Francisco, CA 94143-0450, USA.
Shogren-Knaak
M A
MA
Shokat
K M
KM
D055598:Chemical Phenomena; D002621:Chemistry; D005823:Genetics; D009154:Mutation; D015398:Signal Transduction