title: Changes in activities of enzymes related to malate-aspartate shuttle in leukocytes from dogs given a herb supplement.
abstract: Changes in activities of enzymes related to the malate-aspartate shuttle were measured in leukocytes (WBC) from dogs given food supplemented with 2 mg/kg of herb powder, Echevaria glauca, every day for 4 weeks. There were no significant differences in plasma concentrations of glucose, immunoreactive insulin, free fatty acids and triglyceride between dogs given food with or without the herb supplementation. Activities of malate dehydrogenase (MDH) and aspartate aminotransferase (AST) in the malate-aspartate shuttle increased remarkably in mitochondria of WBC from dogs fed the herb supplementation. It is suggested that Echevaria glauca herb supplementation might activate NADH shuttle systems and mitochondrial energy metabolism in dogs.
authors.affiliation: Division of Veterinary Biochemistry, Nippon Veterinary and Animal Science University, Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Arai
authors.forename: M
authors.initials: M
authors.lastname: Takahashi
authors.forename: S
authors.initials: S
authors.lastname: Kamata
authors.forename: T
authors.initials: T
authors.lastname: Sako
mesh_terms: D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001224:Aspartic Acid / Q000097:blood*; D001786:Blood Glucose / Q000187:drug effects / Q000378:metabolism; D019587:Dietary Supplements; D004285:Dogs / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005260:Female; D005969:Glutamate Dehydrogenase / Q000097:blood; D007328:Insulin / Q000097:blood; D007770:L-Lactate Dehydrogenase / Q000097:blood; D007962:Leukocytes / Q000201:enzymology*; D008291:Malate Dehydrogenase / Q000097:blood; D008293:Malates / Q000097:blood*; D008297:Male; D009243:NAD / Q000097:blood; D014280:Triglycerides / Q000097:blood
chemical_list: D001786:Blood Glucose; D004365:Drugs, Chinese Herbal; D005230:Fatty Acids, Nonesterified; D007328:Insulin; D008293:Malates; D014280:Triglycerides; D009243:NAD; D001224:Aspartic Acid; C030298:malic acid; D007770:L-Lactate Dehydrogenase; D008291:Malate Dehydrogenase; D005969:Glutamate Dehydrogenase; D001219:Aspartate Aminotransferases