Genetic relationships among serogroup B: serotype 4 Neisseria meningitidis strains.
We compared the results obtained by serotyping of PorB epitopes using an expanded panel of monoclonal antibodies (mAb) including mAb 7 and mAb 10, with results obtained by RFLP of rRNA genes (ribotyping). The purpose of this study was to assess the correlation between phenotypic- and genotypic- methods for typing N. meningitidis. The ribotypes obtained using ClaI or EcoRV endonucleases grouped the strains in seven and two different patterns, respectively. This additional characterization of PorB epitopes improved the correlation between these two methods of typing N. meningitidis.
Lemos
A P
AP
Division of Medical Biology, Bacteriology Department, Adolfo Lutz Institute, São Paulo, SP, Brazil. apaula@ial.sp.gov.br
Sacchi
C T
CT
Paiva
M V
MV
Yara
T I
TI
Melles
C E
CE
Mayer
L W
LW
D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D014644:Genetic Variation; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000235:genetics; D009345:Neisseria meningitidis / Q000145:classification / Q000235:genetics*; D018272:Porins; D021521:Ribotyping; D012703:Serotyping
D001425:Bacterial Outer Membrane Proteins; D007135:Immunoglobulin Variable Region; D018272:Porins; C057699:porin protein, Neisseria