The preparation of the chronic hyper-endotoxemia experimental animal model by means of a drug delivery system.
A drug delivery system (DDS) consisting of lipopolysaccharide (LPS) as a drug and 2-hydroxyethyl methacrylate (HEMA)-diethylene glycol dimethacrylate (2G) or -polyethylene glycol dimethacrylate (4G, 9G) copolymer was prepared, and used for the efficient preparation of an experimental animal model of chronic hyper-endotoxemia. The release profiles of LPS in the in-vitro test were greatly influenced by the composition of HEMA-2G, 4G, 9G in the copolymer. It was found that LPS release from the DDS continued gradually and constantly throughout 2 weeks. In the in-vivo experiment with rats, the DDS maintained a high blood concentration level of LPS for 3 days. These results strongly suggest the possibility of convenient and reproducible preparation of a chronic hyper-endotoxemia animal model.
Kakinoki
S
S
Interdisciplinary Graduate School of Science and Technology, Kinki University, Higashi-Osaka, Japan.
Yukutake
K
K
Kaetsu
I
I
Uchida
K
K
Sutani
K
K
Kosumi
T
T
Usui
N
N
Yonekura
T
T
Kubota
A
A
Ohyanagi
H
H
D000005:Abdomen; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002908:Chronic Disease; D003432:Cross-Linking Reagents; D004195:Disease Models, Animal; D016503:Drug Delivery Systems; D004343:Drug Implants; D004435:Eating / Q000187:drug effects; D019446:Endotoxemia / Q000097:blood / Q000473:pathology*; D004731:Endotoxins / Q000008:administration & dosage* / Q000493:pharmacokinetics / Q000633:toxicity; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000633:toxicity; D008689:Methacrylates; D011092:Polyethylene Glycols; D011109:Polymethacrylic Acids; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012995:Solubility
D003432:Cross-Linking Reagents; D004343:Drug Implants; D004731:Endotoxins; D008070:Lipopolysaccharides; D008689:Methacrylates; D011109:Polymethacrylic Acids; C059469:copoly(diethylene glycol dimethacrylate-polyethylene glycol 600 dimethacrylate); D011092:Polyethylene Glycols; C005044:hydroxyethyl methacrylate