title: Effects of topical tumoricidal agents on port-site recurrence of colon cancer: an experimental study in rats.
abstract: BACKGROUND
  Reports of metastatic spread of colon and rectal cancer to port sites after laparoscopic resection of potentially curable lesions has raised doubt regarding the efficacy and safety of laparoscopic technology in cancer surgery. Experimental study in animals has led us to believe that the mode of spread of these metastases is via the direct route. We hypothesized, therefore, that we could decrease the rate of trocar-site recurrences by treating the individual port sites with a topical tumoricidal agent.
  
  
  METHODS
  Male BD-IX rats weighing 240 to 360 g were injected with syngeneic colon cancer to simulate free intraperitoneal cancer spread to trocar sites. All rats were subjected to a sham laparoscopic operation after 2 x 10(5) viable cancer cells had been injected into their peritoneal cavities. Five-millimeter trocars were inserted into each rat after abdominal insufflation to 10 mm Hg. Pneumoperitoneum was maintained for 10 minutes before the trocars were removed simultaneously. Trocar sites were then subjected to one of three treatments, with each animal receiving a maximum of two different treatments. Sites were treated with 70% ethanol (N = 42), povidine/ iodine (N = 40), or no topical treatment (N = 46). Three weeks later, the animals were euthanized and autopsied. Subcutaneous tumors at trocar sites or tumors with >50% volume within the wound were considered implants.
  
  
  RESULTS
  Control sites revealed a metastasis rate of 41% (19/46). The tumor implant rate was 36% (15/42) at alcohol-treated sites and 20% (8/40) at sites treated with povidone-iodine (P < 0.05).
  
  
  CONCLUSIONS
  Topical administration of povidone-iodine to trocar wounds after laparoscopic surgery can significantly reduce the incidence of port-site metastasis in a syngeneic animal model of colon cancer.
authors.affiliation: Division of Colorectal Surgery, University of Southern California, Los Angeles, 90033, USA.
authors.forename: W
authors.initials: W
authors.lastname: Hoffstetter
authors.forename: A
authors.initials: A
authors.lastname: Ortega
authors.forename: M
authors.initials: M
authors.lastname: Chiang
authors.forename: P
authors.initials: P
authors.lastname: Paik
authors.forename: R W
authors.initials: RW
authors.lastname: Beart
mesh_terms: D000287:Administration, Topical; D000438:Alcohols / Q000627:therapeutic use; D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D003110:Colonic Neoplasms / Q000188:drug therapy*; D004195:Disease Models, Animal; D008297:Male; D009364:Neoplasm Recurrence, Local / Q000188:drug therapy*; D009366:Neoplasm Seeding; D011206:Povidone-Iodine / Q000627:therapeutic use; D051381:Rats
chemical_list: D000438:Alcohols; D000970:Antineoplastic Agents; D011206:Povidone-Iodine