Cloning and expression of CitPDS1, a gene encoding phytoene desaturase in citrus.
A cDNA clone encoding phytoene desaturase (PDS) was isolated from citrus (Citrus unshiu Marc.). The transcript of the isolated PDS (CitPDS1) was not detected by conventional RNA gel-blot analysis; instead, it was detected by a sensitive reverse transcription-PCR (RT-PCR). The CitPDS1 transcript in the juice sacs/segment epidermis (edible part) was at a low level in the young fruit, and it increased toward maturation like citrus phytoene synthase (CitPSY1). In the peel, in contrast to CitPSY1, the transcript of which was induced toward maturation, the level of the CitPDS1 transcript remained constant after an increase in July, indicating non-coordinate regulation of CitPDS1 and CitPSY1 in the peel.
Kita
M
M
Department of Citriculture, National Institute of Fruit Tree Science, Shimizu, Shizuoka, Japan.
Komatsu
A
A
Omura
M
M
Yano
M
M
Ikoma
Y
Y
Moriguchi
T
T
D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D002957:Citrus / Q000235:genetics*; D003001:Cloning, Molecular; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics*; D018506:Gene Expression Regulation, Plant / Q000235:genetics*; D017343:Genes, Plant / Q000235:genetics*; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000096:biosynthesis* / Q000235:genetics*; D019441:Plant Epidermis / Q000201:enzymology; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
D012333:RNA, Messenger; D010088:Oxidoreductases; C022398:phytoene dehydrogenase