Homologues of natural killer cell receptors NKG2-D and NKR-P1 expressed in cattle.
Partial transcripts of the homologues in cattle, of the genes encoding the NKR-P1 and NKG2-D natural killer cell lectin-like receptor families, were cloned by reverse transcriptase-PCR from bovine spleen. Three different cDNAs were partially sequenced for the NKG2-D homologue, and two for the NKR-P1 homologue. Identity to human nucleotide sequences was of 90 and 75%, respectively, and all structural residues of C-type lectin carbohydrate recognition domains were conserved. The identification of two of its members allows to hypothesise the existence of a bovine NK gene complex, prospectively located on chromosome 5.
Govaerts
M M
MM
Laboratory of Physiology and Immunology of Domestic Animals, Faculty of Agricultural and Applied Biological Sciences, Katholieke Universiteit Leuven, Kasteelpark Arenberg 30, B-3001 Leuven, Belgium.
Goddeeris
B M
BM
D000595:Amino Acid Sequence; D000818:Animals; D000954:Antigens, Surface / Q000235:genetics*; D001483:Base Sequence; D002417:Cattle / Q000235:genetics* / Q000276:immunology*; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology*; D037181:Lectins, C-Type; D008297:Male; D008969:Molecular Sequence Data; D055651:NK Cell Lectin-Like Receptor Subfamily B; D055654:NK Cell Lectin-Like Receptor Subfamily C; D055655:NK Cell Lectin-Like Receptor Subfamily K; D051381:Rats; D017208:Rats, Wistar; D011971:Receptors, Immunologic / Q000235:genetics*; D055607:Receptors, Natural Killer Cell; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
D000954:Antigens, Surface; D017931:DNA Primers; D018076:DNA, Complementary; C527203:KLRB1 protein, human; C527054:KLRC1 protein, human; C527085:KLRK1 protein, human; D037181:Lectins, C-Type; D055651:NK Cell Lectin-Like Receptor Subfamily B; D055654:NK Cell Lectin-Like Receptor Subfamily C; D055655:NK Cell Lectin-Like Receptor Subfamily K; D011971:Receptors, Immunologic; D055607:Receptors, Natural Killer Cell