title: Construction and characterization of genetically defined aro omp mutants of enterotoxigenic Escherichia coli and preliminary studies of safety and immunogenicity in humans.
abstract: Enterotoxigenic Escherichia coli (ETEC) is a leading cause of diarrhea in travelers to countries where the disease is endemic and causes a major disease burden in the indigenous population, particularly children. We describe here the generation and preclinical characterization of candidate strains of ETEC which are intended to provide the basis of a live attenuated oral vaccine to prevent this disease. It has been shown previously that a spontaneously arising toxin-negative variant ETEC strain, E1392/75-2A, could confer 75% protection against challenge when administered to volunteers. Unfortunately this strain induced mild diarrhea in 15% of recipients. To eliminate the unacceptable reactogenicity of strain E1392/75-2A, it was further attenuated by introducing three different combinations of defined deletion mutations into the chromosome. A mouse intranasal model of immunization was developed and used to show that all of the strains were immunogenic. Immune responses against colonization factor antigens (CFAs) were particularly strong when the bacterial inocula were grown on "CFA agar," which induces strong expression of these antigens. Two of the strains were selected for a phase I dose escalation safety study with healthy adult volunteers. Freshly grown organisms were harvested from CFA agar plates and administered to volunteers as a suspension containing from 5 x 10(7) to 5 x 10(9) CFU. The vaccine was well tolerated at all doses and induced significant immune responses in all recipients at the highest dose of either strain. The results provide the basis for further clinical evaluation of these vaccine candidates.
authors.affiliation: Acambis Ltd., Cambridge CB1 9PT, United Kingdom.
authors.forename: A K
authors.initials: AK
authors.lastname: Turner
authors.forename: T D
authors.initials: TD
authors.lastname: Terry
authors.forename: D A
authors.initials: DA
authors.lastname: Sack
authors.forename: P
authors.initials: P
authors.lastname: Londoño-Arcila
authors.forename: M J
authors.initials: MJ
authors.lastname: Darsley
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D001426:Bacterial Proteins / Q000276:immunology; D001483:Base Sequence; D002876:Chromosomes, Bacterial; D003257:Consumer Product Safety; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development / Q000276:immunology* / Q000472:pathogenicity; D022361:Escherichia coli Vaccines / Q000235:genetics / Q000276:immunology*; D036781:Fimbriae Proteins; D006360:Heat-Shock Proteins; D006801:Humans; D008070:Lipopolysaccharides / Q000096:biosynthesis; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D016296:Mutagenesis; D033903:Periplasmic Proteins; D010641:Phenotype; D019761:Phosphorus-Oxygen Lyases / Q000235:genetics / Q000276:immunology; D010957:Plasmids; D018272:Porins / Q000235:genetics / Q000276:immunology; D012697:Serine Endopeptidases / Q000235:genetics / Q000276:immunology; D015534:Trans-Activators / Q000235:genetics / Q000276:immunology; D014611:Vaccination; D014614:Vaccines, Synthetic / Q000235:genetics / Q000276:immunology*; D014774:Virulence; D014838:Volunteers
chemical_list: D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D022361:Escherichia coli Vaccines; D006360:Heat-Shock Proteins; D008070:Lipopolysaccharides; C113724:OmpC protein; C109237:OmpF protein; D033903:Periplasmic Proteins; D018272:Porins; D015534:Trans-Activators; D014614:Vaccines, Synthetic; C022924:colonization factor antigens; C112177:osmolarity response regulator proteins; D036781:Fimbriae Proteins; C061682:DegP protease; D012697:Serine Endopeptidases; C019895:chorismate synthase; D019761:Phosphorus-Oxygen Lyases