Identification of three new splice variants of the SNARE protein SNAP-23.
SNAP-23 has an important role in protein-trafficking processes in mammalian cells and until yet two isoforms of SNAP-23 (SNAP-23a and SNAP-23b) have been described. In the present report, we have identified the existence of three new SNAP-23 isoforms (named SNAP-23c, SNAP-23d, and SNAP-23e), which arise from alternative splicing. By RT-PCR all five splice variants were shown to be expressed in four different human inflammatory cells (eosinophils, basophils, neutrophils, and peripheral blood mononuclear cells). Transfection of the human basophilic KU-812 cell line with plasmid constructs containing the cDNAs of the five splice variants located SNAP-23a and SNAP-23b primarily in the plasma membrane. The other three splice variants were localized both intracellularly and in the plasma membrane.
Shukla
A
A
Department of Respiratory Diseases, Aarhus University Hospital, DK-8000 Aarhus, Denmark.
Corydon
T J
TJ
Nielsen
S
S
Hoffmann
H J
HJ
Dahl
R
R
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001491:Basophils / Q000378:metabolism; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics*; D002460:Cell Line; D004804:Eosinophils / Q000378:metabolism; D005091:Exons; D014644:Genetic Variation; D006098:Granulocytes / Q000378:metabolism*; D006801:Humans; D007438:Introns; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008322:Mammals; D008969:Molecular Sequence Data; D009504:Neutrophils / Q000378:metabolism; D020033:Protein Isoforms / Q000737:chemistry / Q000235:genetics; D050766:Qb-SNARE Proteins; D050767:Qc-SNARE Proteins; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic; D014162:Transfection
D002352:Carrier Proteins; D020033:Protein Isoforms; D050766:Qb-SNARE Proteins; D050767:Qc-SNARE Proteins; D011993:Recombinant Fusion Proteins; C100493:SNAP23 protein, human