[Oncogenic human papillomaviruses in extra-genital Bowen disease revealed by in situ hybridization].
BACKGROUND
The association between mucosal oncogenic human papillomaviruses (HPV) and bowenoid papulosis or genital Bowen's disease is well documented. In contrast this association with extra-genital Bowen's disease is poorly studied. The aim of this study was to detect oncogenic (16/18, 31/33/51) and non oncogenic (8/11) mucosal HPV using a in situ hybridization method in 28 skin biopsy specimens of extra-genital Bowen's disease.


METHODS
Twenty-eight cases of extra-genital Bowen's disease seen in the period 1990-96 in the Dermatology department were included: 19 women and 9 men (mean age: 72 years). Bowen's disease locations were: hands and feet (8 cases), limbs (11 cases), face (8 cases), trunk (1 case). Blinded histopathologic examination confirmed the diagnosis of Bowen's disease and signs of HPV infection (koilocytosis). In situ hybridization was performed using three biotinylated probes detecting HPV types 6/11, 16/18, 31/33/51.


RESULTS
Oncogenic HPV genoma was detected in 8 skin samples (28.6 p. 100). In all these cases, 16/18 probe was positive and in two cases, both 16/18 and 31/33/51 probes were positive; 4/8 Bowen's diseases of the extremities were positive for HPV. Koilocytes were found in 6/8 of skin samples with positive HPV detection.


CONCLUSIONS
Mucosal oncogenic HPV are detected by in situ hybridization in 28.6 p. 100 of extra-genital Bowen's disease. In situ hybridization is an easier technique than Southern-Blot hybridization which is the gold standard. Five studies reported similar results and three studies reported different results that we discuss. A precise understanding of oncogenic HPV implication in the development of extra-genital Bowen's disease could lead to the development of new therapeutic strategies (topical cidofovir or imiquimod).
Derancourt
C
C
Service de Dermatologie, CHU, Reims, Cedex, France.
Mougin
C
C
Chopard Lallier
M
M
Coumes-Marquet
S
S
Drobacheff
C
C
Laurent
R
R
D000368:Aged; D001913:Bowen's Disease / Q000473:pathology / Q000821:virology*; D005260:Female; D005833:Genital Neoplasms, Female / Q000473:pathology / Q000821:virology; D005834:Genital Neoplasms, Male / Q000473:pathology / Q000821:virology; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D027383:Papillomaviridae / Q000302:isolation & purification / Q000472:pathogenicity*; D030361:Papillomavirus Infections / Q000473:pathology / Q000821:virology*; D012680:Sensitivity and Specificity; D012867:Skin / Q000473:pathology / Q000821:virology; D012878:Skin Neoplasms / Q000473:pathology / Q000821:virology*; D014412:Tumor Virus Infections / Q000473:pathology / Q000821:virology*; D014774:Virulence