Analysis of mutations in the p53 tumor suppressor gene and Ki- and Ha-ras proto-oncogenes in hepatic tumors of European flounder (Platichthys flesus).
DNA from five tumors, three other-injured livers and two normal liver tissue samples from the European flounder were analyzed for mutations in exons 5-8 of the tumor suppressor gene p53 and at codons 12, 13 and 61 of Ha- and Ki-ras proto-oncogenes. No tumor-specific mutations were identified by direct sequencing and single-strand conformation polymorphism of these genes. A number of silent polymorphisms were noted in p53. In addition to a need for more extensive analyses of flounder liver tumor samples for ras or p53 mutations, other cancer-related genes should be investigated.
Franklin
T M
TM
School of Biosciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK.
Lee
J S
JS
Köhler
A
A
Chipman
J K
JK
D000818:Animals; D005091:Exons; D005393:Fish Diseases / Q000235:genetics*; D005432:Flounder; D011905:Genes, ras / Q000235:genetics*; D008113:Liver Neoplasms / Q000235:genetics / Q000662:veterinary*; D009154:Mutation; D011110:Polymorphism, Genetic; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*
D016159:Tumor Suppressor Protein p53