Protein expression signatures identified in Mytilus edulis exposed to PCBs, copper and salinity stress.
Applied to environmental toxicology, proteome analysis may be used to isolate chemical-specific protein expression signatures (PES). In this project specific PES were isolated in mussels, Mytilus edulis, from the Baltic Sea subjected in the laboratory to treatment with copper (70 ppb), Aroclor 1248 (1 ppb), and to lowered salinity. Four mussels in each treatment group were acclimated in the laboratory for 24 h before beginning the 7-day exposure. Whole body tissue was homogenized and separated using two-dimensional gel electrophoresis. The protein gels were scanned to TIFF files and compared using MELANIE II 2D gel analysis software (BioRad). Protein expression signatures including proteins induced and repressed by exposure were isolated for each treatment group. The specificity of PES due to environmental changes shows promise in bioindication, toxicity testing and in helping identify possible toxicity mechanisms.
Shepard
J L
JL
Department of Biological Sciences, University of Maryland (UMBC), 1000 Hilltop Circle, Baltimore, MD 21250, USA.
Olsson
B
B
Tedengren
M
M
Bradley
B P
BP
D000818:Animals; D001140:Aroclors / Q000633:toxicity; D049872:Bivalvia / Q000187:drug effects*; D003300:Copper / Q000633:toxicity*; D015180:Electrophoresis, Gel, Two-Dimensional; D011078:Polychlorinated Biphenyls / Q000633:toxicity*; D014176:Protein Biosynthesis; D012965:Sodium Chloride / Q000008:administration & dosage / Q000633:toxicity*; D014116:Toxicology / Q000379:methods*
D001140:Aroclors; C028617:aroclor 1248; D012965:Sodium Chloride; D003300:Copper; D011078:Polychlorinated Biphenyls