Cloning and characterisation of a hyp gene cluster in the filamentous cyanobacterium Nostoc sp. strain PCC 73102.
Maturation of [NiFe]-hydrogenases requires the action of several groups of accessory genes. Homologues of one group of these genes, the so-called hyp genes, putatively encoding proteins participating in the formation of an active uptake hydrogenase in the filamentous, heterocyst-forming cyanobacterium Nostoc PCC 73102, were cloned. The cluster, consisting of hypF, hypC, hypD, hypE, hypA, and hypB, is located 3.8 kb upstream from the uptake hydrogenase-encoding hupSL. Gene expression analyses show that these hyp genes are, like hupL, transcribed under N(2)-fixing but not under non-N(2)-fixing growth conditions. Furthermore, the six hyp genes are transcribed together with an open reading frame upstream of hypF, as a single mRNA. Analysis of the DNA region upstream of the experimentally determined transcriptional start site revealed putative -10 and -35 sequence elements and putative binding sites for the global nitrogen regulator NtcA.
Hansel
A
A
Department of Physiological Botany, EBC, Uppsala University, Sweden.
Axelsson
R
R
Lindberg
P
P
Troshina
O Y
OY
Wünschiers
R
R
Lindblad
P
P
D020816:Amino Acid Motifs; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics; D001483:Base Sequence; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics; D003001:Cloning, Molecular; D000458:Cyanobacteria / Q000201:enzymology / Q000235:genetics*; D019204:GTP-Binding Proteins / Q000737:chemistry / Q000235:genetics; D015870:Gene Expression; D005798:Genes, Bacterial; D006864:Hydrogenase / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D005810:Multigene Family; D009586:Nitrogen Fixation; D016366:Open Reading Frames; D011506:Proteins; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020539:Sequence Analysis, Protein; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D002352:Carrier Proteins; C089637:HypB protein, bacteria; C108874:HypC protein, Bacteria; C108875:HypD protein, Bacteria; C108878:HypF protein, Bacteria; D011506:Proteins; C085871:hypE protein, Bacteria; D006864:Hydrogenase; D019204:GTP-Binding Proteins