title: Drosophila SAP18, a member of the Sin3/Rpd3 histone deacetylase complex, interacts with Bicoid and inhibits its activity.
abstract: Bicoid directs anterior development in Drosophila embryos by activating different genes along the anterior-posterior axis. However, its activity is down-regulated at the anterior tip of the embryo, in a process known as retraction. Retraction is under the control of the terminal polarity system, and results in localized repression of Bicoid target genes. Here, we describe a Drosophila homolog of human SAP18, a member of the Sin3A/Rpd3 histone deacetylase complex. dSAP18 interacts with Bicoid in yeast and in vitro, and is expressed early in development coincident with Bicoid. In tissue culture cells, dSAP18 inhibits the ability of Bicoid to activate reporter genes. These results suggest a model in which dSAP18 interacts with Bicoid to silence expression of Bicoid target genes in the anterior tip of the embryo.
authors.affiliation: Molecular Genetics Program, Wadsworth Center, New York State Department of Health & Department of Biomedical Sciences, School of Public Health, State University of New York, Albany, NY 12208, USA. hanes@wadsworth.org
authors.forename: W
authors.initials: W
authors.lastname: Zhu
authors.forename: M
authors.initials: M
authors.lastname: Foehr
authors.forename: J B
authors.initials: JB
authors.lastname: Jaynes
authors.forename: S D
authors.initials: SD
authors.lastname: Hanes
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000071183:Autophagy-Related Proteins; D001483:Base Sequence; D002352:Carrier Proteins; D002874:Chromosome Mapping; D056970:Co-Repressor Proteins; D004247:DNA; D004330:Drosophila / Q000196:embryology*; D029721:Drosophila Proteins; D018507:Gene Expression Regulation, Developmental; D006655:Histone Deacetylases / Q000378:metabolism*; D018398:Homeodomain Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D011485:Protein Binding; D016601:RNA-Binding Proteins; D029701:Saccharomyces cerevisiae Proteins; D017386:Sequence Homology, Amino Acid; D015534:Trans-Activators / Q000037:antagonists & inhibitors / Q000378:metabolism*; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: C498649:ATG16 protein, S cerevisiae; D000071183:Autophagy-Related Proteins; D002352:Carrier Proteins; D056970:Co-Repressor Proteins; D029721:Drosophila Proteins; D018398:Homeodomain Proteins; D016601:RNA-Binding Proteins; C498650:SAP18 protein, human; D029701:Saccharomyces cerevisiae Proteins; D015534:Trans-Activators; D014157:Transcription Factors; C056478:bcd protein, Drosophila; C407043:bicoid interacting protein 1, Drosophila; D004247:DNA; D006655:Histone Deacetylases