title: Accuracy of thin-layer cytology in patients undergoing cervical cone biopsy.
abstract: OBJECTIVE
  To compare the accuracy of thin-layer cytology with Autocyte PREP (TriPath Imaging Inc., Burlington, North Carolina, U.S.A.) with conventional smears in 500 women undergoing cervical cone biopsy.
  
  
  METHODS
  The study was performed among 500 consecutive women presenting for cone biopsy for high grade cervical intraepithelial neoplasia (CIN) on biopsy in 350 (70%) and discrepant cytology/colpohistology in 150 (30%). Before performing a cone biopsy, two cervical samples were collected for conventional smears and thin-layer cytologic slides, with randomization of the order. Conventional smears were stained and diagnosed at Pasteur Cerba, while thin-layer cytologic slides were processed at a local TriPath office (Meylan, France) and sent in a masked fashion for screening at Pasteur Cerba. Any slides initially read as normal were reviewed again and reported without knowledge of the other cytologic or cone biopsy data. The final cytologic diagnoses for the two methods were compared with histopathology of the cone biopsy.
  
  
  RESULTS
  The conventional smear was unsatisfactory in 58 (11.6%) of cases, while there were 4 (0.8%) unsatisfactory thin-layer cytologic slides (P < .001). Endocervical cells were missing from 31 (6.2%) of conventional smears and 34 (6.8%) of thin-layer cytologic slides. For the pooled data, sensitivities of conventional smear and thin layer for detecting high grade CIN (0.82% and 0.86%, respectively) were similar as were specificities (0.40% and 0.43%, respectively). When first samples were compared, the sensitivities of the conventional smear and thin layer for high grade CIN were 0.79% and 0.89%, respectively (P = .02), with corresponding specificities of 0.41% and 0.36% (P < .01).
  
  
  CONCLUSIONS
  When controlled for sample order, the sensitivity of thin-layer cytology for detecting high grade CIN was significantly higher than that of conventional smears in patients with previous abnormal cytology, but at the expense of specificity.
authors.affiliation: Laboratoire Pasteur Cerba, Cergy Pontoise, and Institut Curie and Hopital Bichat, Paris, France. bergeron@pasteur-cerba.com
authors.forename: C
authors.initials: C
authors.lastname: Bergeron
authors.forename: J
authors.initials: J
authors.lastname: Bishop
authors.forename: A
authors.initials: A
authors.lastname: Lemarie
authors.forename: F
authors.initials: F
authors.lastname: Cas
authors.forename: J
authors.initials: J
authors.lastname: Ayivi
authors.forename: B
authors.initials: B
authors.lastname: Huynh
authors.forename: R
authors.initials: R
authors.lastname: Barrasso
mesh_terms: D001706:Biopsy / Q000379:methods; D002584:Cervix Uteri / Q000473:pathology / Q000601:surgery; D005260:Female; D016591:Histocytological Preparation Techniques / Q000379:methods*; D006801:Humans; D012680:Sensitivity and Specificity; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis / Q000473:pathology*; D014626:Vaginal Smears / Q000379:methods*; D002578:Uterine Cervical Dysplasia / Q000175:diagnosis / Q000473:pathology*