title: Detection of ehrlichial infection by PCR in dogs from Yamaguchi and Okinawa Prefectures, Japan.
abstract: Species-specific nested polymerase chain reaction (PCR) was used to detect the presence of possible canine ehrlichial agents (Ehrlichia canis, E. chaffeensis, E. ewingii, E. equi and E. platys) and monocytic ehrlichial agents found in Japan (E. muris and a recently discovered Ehrlichia species detected from Ixodes ovatus) in blood samples from dogs in Yamaguchi and Okinawa Prefecture, Japan. Partial sequence of E. platys was detected from 1 of 67 dogs (1.5%) tested from Yamaguchi Prefecture and 24 out of 87 (27.6%) in the subtropical Okinawa Prefecture. Dogs in Okinawa and Miyako Islands had a higher positive rate (69.2 and 45.0%, respectively) than Ishigaki Island (11.1%). Another dog in Yamaguchi Prefecture had a positive PCR reaction to the Ehrlichia sp. detected from I. ovatus. No other Ehrlichia were found in these samples.
authors.affiliation: Laboratory of Veterinary Internal Medicine, Faculty of Agriculture, Yamaguchi University, Yoshida, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Inokuma
authors.forename: K
authors.initials: K
authors.lastname: Ohno
authors.forename: T
authors.initials: T
authors.lastname: Onishi
authors.forename: D
authors.initials: D
authors.lastname: Raoult
authors.forename: P
authors.initials: P
authors.lastname: Brouqui
mesh_terms: D000818:Animals; D016054:DNA, Protozoan / Q000097:blood / Q000235:genetics; D004283:Dog Diseases / Q000453:epidemiology / Q000469:parasitology*; D004285:Dogs; D004536:Ehrlichia / Q000235:genetics* / Q000302:isolation & purification; D016873:Ehrlichiosis / Q000097:blood / Q000175:diagnosis / Q000453:epidemiology / Q000662:veterinary*; D005260:Female; D007564:Japan / Q000453:epidemiology; D008297:Male; D016133:Polymerase Chain Reaction / Q000662:veterinary*; D015995:Prevalence; D017422:Sequence Analysis, DNA
chemical_list: D016054:DNA, Protozoan