Neuromodulator content of hamster intergeniculate leaflet neurons and their projection to the suprachiasmatic nucleus or visual midbrain.
The intergeniculate leaflet (IGL) of the lateral geniculate complex has widespread, bilateral, and reciprocal connections with nuclei in the subcortical visual shell. Its function is poorly understood with respect to its role in visual processing. The most well-known IGL projection, and the only one with a clear function, is the geniculohypothalamic tract (GHT) that terminates in the suprachiasmatic nucleus (SCN), site of the primary circadian clock. The hamster GHT is derived, in part, from IGL neurons containing neuropeptide Y and enkephalin. IGL neurons containing these peptides also project to the pretectal region. The present studies used a combination of immunohistochemical, lesion, and retrograde tracing techniques to study neuron types in the IGL and their projections to hamster SCN and pretectum. Two additional neuromodulators, gamma-aminobutyric acid (GABA) and neurotensin, are shown to be present in IGL neurons. The GABA- and neurotensin-immunoreactive neurons project to the SCN with terminal field patterns very similar to those for neuropeptide Y and enkephalin. IGL neurons of all four types also send projections to the pretectum, but rarely do individual cells project to both the SCN and the pretectum. Nearly all neurotensin is colocalized with neuropeptide Y in IGL neurons, although about half of the neuropeptide Y cells do not contain neurotensin. Otherwise, the extent to which the four neuromodulators are colocalized varies from 6% to 54%. Nearly every SCN neuron appears to contain GABA. In the IGL, the majority of cells studied are not identifiable by GABA immunoreactivity. Putative functions of the various neuromodulator projections from the IGL to pretectum or SCN are discussed.
Morin
L P
LP
Department of Psychiatry, State University of New York, Stony Brook, NY 11794, USA. lmorin@epo.som.sunysb.edu
Blanchard
J H
JH
D000818:Animals; D002940:Circadian Rhythm; D006224:Cricetinae; D004745:Enkephalins / Q000032:analysis; D005829:Geniculate Bodies / Q000737:chemistry / Q000166:cytology*; D008297:Male; D008647:Mesocricetus / Q000033:anatomy & histology*; D009474:Neurons / Q000737:chemistry* / Q000166:cytology*; D009478:Neuropeptide Y / Q000032:analysis; D009479:Neuropeptides / Q000032:analysis*; D009496:Neurotensin / Q000032:analysis; D013477:Superior Colliculi / Q000737:chemistry / Q000166:cytology; D013493:Suprachiasmatic Nucleus / Q000737:chemistry / Q000166:cytology*; D014795:Visual Pathways / Q000737:chemistry / Q000166:cytology; D005680:gamma-Aminobutyric Acid / Q000032:analysis
D004745:Enkephalins; D009478:Neuropeptide Y; D009479:Neuropeptides; D009496:Neurotensin; D005680:gamma-Aminobutyric Acid