Development of a simple gradient LC-IR interface for the detection of terpenoids from the alpha-pinene-ozone reaction.
The development of a simple interface between liquid chromatography and infrared spectroscopy (LC-IR) using a coaxial sprayer is described for less volatile analytes. The system consists of a transfer capillary, in which the analytes are transported from the separation column of the gradient-LC to the outlet of the sprayer. This transfer capillary is coaxially surrounded at the outlet by a stainless steel sprayer capillary, which is resistively heated and flushed with nitrogen gas. The samples are sprayed in the manner that the eluent is vaporized by the heated nitrogen when exiting the capillary, while the analytes are deposited on a moving slide made of infrared transparent material (ZnSe or CaF2). Afterwards the deposited compounds are analyzed with an infrared microscope in transmission. First results from reaction products of the gas phase reaction of alpha-pinene with ozone are presented.
Geiger
J
J
Institut für Spektrochemie und Angewandte Spektroskopie (ISAS), Insntutsteil Berlin, Berlin-Adlershof, Germany.
Korte
E H
EH
Schrader
W
W
D000081008:Bicyclic Monoterpenes; D002853:Chromatography, Liquid / Q000379:methods*; D039821:Monoterpenes; D010126:Ozone / Q000737:chemistry*; D013055:Spectrophotometry, Infrared / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet; D013729:Terpenes / Q000032:analysis* / Q000737:chemistry*
D000081008:Bicyclic Monoterpenes; D039821:Monoterpenes; D013729:Terpenes; D010126:Ozone; C005451:alpha-pinene