Effects of Se, Cu and Se + vitamin E deficiency on the activities of CuZnSOD, GSH-Px, CAT and LPO levels in chicken erythrocytes.
Antioxidant enzymes and vitamins provide a defence against the damage of cells by reactive oxygen species in living systems. The effect of Cu, Se and vitamin E deficiencies on the antioxidant enzyme activities and lipid peroxide levels of chicken erythrocytes were investigated during 6 weeks of a depletion diet. CuZnSOD activity and the plasma Cu level of the Cu-deficient group which was fed a diet containing 0.2 mg Cu x kg(-1) were reduced to 62 and 71% respectively. GSH-Px activity of the Se-deficient group was decreased by 46% but by 21% in the Cu-deficient group. CAT activity values of Se- and Cu-deficient groups were increased by 28 and 10% respectively. The maximum increase of LPO levels in erythrocyte membranes was observed as 32% for the Se+E-deficient group. The LPO level of the Cu-deficient group which had decreased CuZnSOD and GSH-Px activity, was also observed to be significantly increased when compared with the controls (p < 0.05).
Bozkaya
L A
LA
University of Dokuz Eylül, Faculty of Science and Art, Department of Chemistry, 35150 Buca, Izmir, Turkey.
Oztürk-Urek
R
R
Aydemir
T
T
Tarhan
L
L
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D002645:Chickens; D003300:Copper / Q000172:deficiency; D004912:Erythrocytes / Q000378:metabolism*; D005979:Glutathione Peroxidase / Q000378:metabolism; D008054:Lipid Peroxides / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism; D012643:Selenium / Q000172:deficiency; D013223:Statistics as Topic; D013482:Superoxide Dismutase / Q000378:metabolism; D013997:Time Factors; D014131:Trace Elements / Q000172:deficiency*; D014810:Vitamin E / Q000627:therapeutic use; D014811:Vitamin E Deficiency / Q000378:metabolism
D000975:Antioxidants; D008054:Lipid Peroxides; D017382:Reactive Oxygen Species; D014131:Trace Elements; D014810:Vitamin E; D003300:Copper; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D012643:Selenium