title: CD8- dendritic cell activation status plays an integral role in influencing Th2 response development.
abstract: Whether dendritic cells (DC) play a passive or active role in Th2 response induction is poorly understood. In this study, we show that CD8- DC pulsed with Th2-polarizing Ag (soluble egg Ag (SEA)) from Schistosoma mansoni potently stimulate Th2 responses in vivo and in vitro while failing to undergo a conventional maturation process. Thus, in contrast to DC pulsed with the Th1 response inducing Ag Propionebacterium acnes, SEA-exposed DC exhibit a phenotype that is most similar to that of immature DC, failing to up-regulate expression of CD40, CD54, CD80, CD86, or OX40L; producing no detectable IL-4, IL-10, or IL-12; and displaying only a minor increase in MHC class II expression. Importantly, in vitro derived DC exposed to SEA were phenotypically similar to CD8- DC isolated from active S. mansoni infection. By discriminating between different types of pathogen and responding appropriately, CD8- DC play a major role in the decision process to mount either a Th1 or Th2 response.
authors.affiliation: Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA.
authors.forename: A S
authors.initials: AS
authors.lastname: MacDonald
authors.forename: A D
authors.initials: AD
authors.lastname: Straw
authors.forename: B
authors.initials: B
authors.lastname: Bauman
authors.forename: E J
authors.initials: EJ
authors.lastname: Pearce
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000494:pharmacology; D000947:Antigens, Helminth / Q000494:pharmacology; D019013:CD40 Antigens / Q000502:physiology; D016827:CD8 Antigens / Q000096:biosynthesis; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology / Q000469:parasitology; D005260:Female; D016130:Immunophenotyping; D018664:Interleukin-12 / Q000096:biosynthesis; D051379:Mice; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D011425:Propionibacterium acnes / Q000276:immunology; D012550:Schistosoma mansoni / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology*
chemical_list: D000942:Antigens, Bacterial; D000947:Antigens, Helminth; D019013:CD40 Antigens; D016827:CD8 Antigens; D018664:Interleukin-12