Beet necrotic yellow vein virus particles localize to mitochondria during infection.
Fluorescent beet necrotic yellow vein virus (BNYVV) particles were produced by replacing part of the readthrough domain of the minor coat protein P75 with the green fluorescent protein (GFP). The recombinant virus was functional in plants and P75-GFP was incorporated at one end of the rod-shaped virions. Laser scanning confocal microscopy and transmission electron microscopy showed that virus-like particles, almost certainly authentic BNYVV virions, localized to the cytoplasmic surface of mitochondria at early times postinfection but relocated at later times to semiordered clusters in the cytoplasm. This is the first report of specific targeting of plant virus particles to the mitochondria in vivo.
Erhardt
M
M
Institut de Biologie Moléculaire des Plantes, CNRS and Université Louis Pasteur, 12 Rue du Général Zimmer, 67084 Strasbourg Cedex, France.
Dunoyer
P
P
Guilley
H
H
Richards
K
K
Jonard
G
G
Bouzoubaa
S
S
D002213:Capsid / Q000235:genetics / Q000378:metabolism; D019663:Chenopodiaceae / Q000821:virology*; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008854:Microscopy, Electron; D008928:Mitochondria / Q000821:virology*; D010935:Plant Diseases / Q000821:virology*; D010942:Plant Viruses / Q000502:physiology*; D012328:RNA Viruses / Q000502:physiology*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D013347:Subcellular Fractions / Q000821:virology; D014771:Virion / Q000378:metabolism*
D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins