title: Comparison of drug substance impurity profiles generated with extended length columns during packed-column SFC.
abstract: The current study assesses the effect of extending column length during gradient packed column sub/supercritical fluid chromatography (PCSFC) experiments on the detection of known and unknown impurities in a drug substance sample. Quantitative drug substance impurity profiles were generated and compared using multiple column PCSFC and HPLC conditions. Also, chromatographic figures of merit were estimated and compared for components of a standard mixture during PCSFC experiments, which used one column, four columns, and six columns in series.
authors.affiliation: Pharmacia Research and Development, 4901 Searle Parkway, Skokie, IL 60077, USA. daryl.a.roston@pharmacia.com
authors.forename: D A
authors.initials: DA
authors.lastname: Roston
authors.forename: S
authors.initials: S
authors.lastname: Ahmed
authors.forename: D
authors.initials: D
authors.lastname: Williams
authors.forename: T
authors.initials: T
authors.lastname: Catalano
mesh_terms: D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D004340:Drug Contamination; D004364:Pharmaceutical Preparations / Q000032:analysis*
chemical_list: D004364:Pharmaceutical Preparations