Acidic polysaccharides from rhizomes of Atractylodes lancea as protective principle in Candida-lnfected mice.
Prophylactic effects upon imunnosuppressed mice lethally infected by Candida albicans were examined in fractions prepared from a constituent herb of Juzen-taiho-to (TJ-48, Si-Quan-Da-Bu-Tang), rhizomes of Atractylodes lancea DC. The oral administration of water extract obtained from a residue after MeOH extraction of rhizomes significantly prolonged the survival period of the infected mice at a dose of 140 mg/kg/day compared with control mice, while the MeOH extract did not. In the crude polysaccharide fraction (F-2) obtained by EtOH precipitation of the water extract, a significant life-prolonging effect was observed by the administration of 70 mg/kg/day. F-2 was further fractionated, and the resulting strongly acidic polysaccharide fraction, F-2-2, had a protective effect at a dose of 17.5 mg/kg/day. This fraction mainly consisted of acidic pectic polysaccharides containing about 80% galacturonic acid. The protective activity of F-2-2 was lost by periodate oxidation, but not by protease digestion, suggesting that the polysaccharide component of F-2-2 plays a major role in the protective activity against Candida-infected mice.
Inagaki
N
N
Kampo & Pharmacognosy Laboratory, Tsumura & Co., Ibaraki, Japan. inagaki_nobuyuki@mail.tsumura.co.jp
Komatsu
Y
Y
Sasaki
H
H
Kiyohara
H
H
Yamada
H
H
Ishibashi
H
H
Tansho
S
S
Yamaguchi
H
H
Abe
S
S
D000818:Animals; D000935:Antifungal Agents / Q000302:isolation & purification / Q000494:pharmacology*; D019659:Asteraceae / Q000737:chemistry*; D002177:Candidiasis / Q000188:drug therapy* / Q000276:immunology; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology*; D005260:Female; D016867:Immunocompromised Host; D020835:Medicine, Kampo; D051379:Mice; D008517:Phytotherapy; D018547:Plant Stems; D010946:Plants, Medicinal; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*
D000935:Antifungal Agents; D004365:Drugs, Chinese Herbal; D011134:Polysaccharides; C063587:juzentaihoto