title: A follow-up study of the human class and subclass antibody response developed against the adult stage of Trichinella spiralis.
abstract: We report the analysis by ELISA of class and subclass antibody response against a total soluble extract from T. spiralis adult stage (TSE-A) during a year after the infection in 17 symptomatic trichinellosis patients (SI) and five asymptomatic individual (AI) involved in an outbreak of trichinellosis occurred in the State of Mexico. Serum samples from 20 healthy individuals (HI) and 24 patients with other parasitosis were included as control. All SI showed a polyisotypic antibody response against the TSE-A, during the infection. Higher response of IgA, IgE, IgM were detected in SI during the acute phase of the infection, but only IgE remained at high levels all along the infection. None or a lower reactivity against TSE-A was observed in sera from AI and from HI. Some patients with trichuriosis and ascariosis showed a higher cross-reactivity, against TSE-A when IgG and their subclasses were analyzed.
authors.affiliation: Immunology Department, Escuela Nacional de Ciencias Biológicas, I.P.N. Apdo. Postal CON-238, Mexico, D.F. c.p. 06400 Mexico. mchapa@redipn.ipn.mx
authors.forename: M R
authors.initials: MR
authors.lastname: Chapa-Ruiz
authors.forename: D
authors.initials: D
authors.lastname: González-Pantaleón
authors.forename: A
authors.initials: A
authors.lastname: Morales-Galán
authors.forename: A
authors.initials: A
authors.lastname: Contreras-Ramos
authors.forename: M R
authors.initials: MR
authors.lastname: Salinas-Tobón
authors.forename: R
authors.initials: R
authors.lastname: Martínez Y Zamora
mesh_terms: D000328:Adult; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000917:Antibody Formation; D004196:Disease Outbreaks; D004797:Enzyme-Linked Immunosorbent Assay; D005500:Follow-Up Studies; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood; D007073:Immunoglobulin E / Q000097:blood; D007132:Immunoglobulin Isotypes / Q000097:blood*; D007075:Immunoglobulin M / Q000097:blood; D008800:Mexico / Q000453:epidemiology; D051379:Mice; D008807:Mice, Inbred BALB C; D012016:Reference Values; D013997:Time Factors; D017160:Trichinella spiralis / Q000276:immunology*; D014235:Trichinellosis / Q000097:blood / Q000453:epidemiology / Q000276:immunology*
chemical_list: D000909:Antibodies, Helminth; D007070:Immunoglobulin A; D007132:Immunoglobulin Isotypes; D007075:Immunoglobulin M; D007073:Immunoglobulin E