title: Development and mapping of microsatellites from a microdissected BTA 11-specific DNA library.
abstract: A chromosome-specific library was developed for Bos taurus autosome 11 by chromosome microdissection and microcloning using a bovine primary fibroblast culture, obtained from a t(X;23) heifer, that spontaneously developed a translocation chromosome involving bovine chromosome 11. The library was screened using (AC)12 oligos, positive clones selected, sequenced and primers developed to generate bovine chromosome 11-specific microsatellite markers. This study suggests that chromosome-specific libraries have great potential for development of microsatellite markers for the construction of marker-saturated linkage maps for each chromosome.
authors.affiliation: Department of Veterinary & Animal Science, University of Massachusetts, Amherst, MA 01003, USA.
authors.forename: S
authors.initials: S
authors.lastname: Ambady
authors.forename: S M
authors.initials: SM
authors.lastname: Kappes
authors.forename: C
authors.initials: C
authors.lastname: Park
authors.forename: R Z
authors.initials: RZ
authors.lastname: Ma
authors.forename: J E
authors.initials: JE
authors.lastname: Beever
authors.forename: H A
authors.initials: HA
authors.lastname: Lewin
authors.forename: T P
authors.initials: TP
authors.lastname: Smith
authors.forename: C W
authors.initials: CW
authors.lastname: Beattie
authors.forename: P K
authors.initials: PK
authors.lastname: Basrur
authors.forename: F A
authors.initials: FA
authors.lastname: Ponce de León
mesh_terms: D000818:Animals; D002417:Cattle / Q000235:genetics*; D002874:Chromosome Mapping; D005260:Female; D015723:Gene Library; D005819:Genetic Markers; D018895:Microsatellite Repeats; D014178:Translocation, Genetic; D014960:X Chromosome
chemical_list: D005819:Genetic Markers