Cloning and developmental expression of STAT5 in Xenopus laevis.
Transcription factors of the signal transducer and activator of transcription (STAT) family are required for cellular responses to multiple signalling molecules. After ligand binding-induced activation of cognate receptors, STAT proteins are phosphorylated, hetero- or homodimerize, and enter the nucleus. STAT dimers bind to specific DNA elements and alter the transcriptional activity of the signal-responsive genes. We report the cloning and developmental pattern of expression of XSTAT5, a Xenopus laevis member of the STAT family, closely related to the mammalian STAT5A and STAT5B. XSTAT5 is expressed maternally and zygotically. With the onset of neurulation, XSTAT5 RNA are clearly localized in the anterior neural plate and subsequently in the neural structures of the developing eye, the pineal gland and the cement gland anlage. At late tailbud stages, a faint expression is detected in a ventral location that might correspond to the ventral blood islands.
Pascal
A
A
Laboratoire de Biologie Moléculaire et Cellulaire du Développement, Groupe Biologie Expérimentale, UMR CNRS 7622, Université Paris VI, 9 quai Saint-Bernard, 75005, Paris, France.
Riou
J F
JF
Carron
C
C
Boucaut
J C
JC
Umbhauer
M
M
D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D004625:Embryo, Nonmammalian / Q000378:metabolism*; D047108:Embryonic Development; D005123:Eye / Q000196:embryology / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D020869:Gene Expression Profiling; D008969:Molecular Sequence Data; D009420:Nervous System / Q000196:embryology; D010870:Pineal Gland / Q000196:embryology / Q000378:metabolism; D050799:STAT5 Transcription Factor; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D029867:Xenopus Proteins; D014982:Xenopus laevis / Q000196:embryology
D050799:STAT5 Transcription Factor; C435753:STAT5B protein, Xenopus; D014157:Transcription Factors; D029867:Xenopus Proteins