title: Overlapping functions of the pRb family in the regulation of rRNA synthesis.
abstract: The "pocket" proteins pRb, p107, and p130 are a family of negative growth regulators. Previous studies have demonstrated that overexpression of pRb can repress transcription by RNA polymerase (Pol) I. To assess whether pRb performs this role under physiological conditions, we have examined pre-rRNA levels in cells from mice lacking either pRb alone or combinations of the three pocket proteins. Pol I transcription was unaffected in pRb-knockout fibroblasts, but specific disruption of the entire pRb family deregulated rRNA synthesis. Further analysis showed that p130 shares with pRb the ability to repress Pol I transcription, whereas p107 is ineffective in this system. Production of rRNA is abnormally elevated in Rb(-/-) p130(-/-) fibroblasts. Furthermore, overexpression of p130 can inhibit an rRNA promoter both in vitro and in vivo. This reflects an ability of p130 to bind and inactivate the upstream binding factor, UBF. The data imply that rRNA synthesis in living cells is subject to redundant control by endogenous pRb and p130.
authors.affiliation: Division of Molecular Biology of the Cell II, German Cancer Research Centre, Im Neuenheimer Feld 280, D-69120 Heidelberg, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Ciarmatori
authors.forename: P H
authors.initials: PH
authors.lastname: Scott
authors.forename: J E
authors.initials: JE
authors.lastname: Sutcliffe
authors.forename: A
authors.initials: A
authors.lastname: McLees
authors.forename: H M
authors.initials: HM
authors.lastname: Alzuherri
authors.forename: J H
authors.initials: JH
authors.lastname: Dannenberg
authors.forename: H
authors.initials: H
authors.lastname: te Riele
authors.forename: I
authors.initials: I
authors.lastname: Grummt
authors.forename: R
authors.initials: R
authors.lastname: Voit
authors.forename: R J
authors.initials: RJ
authors.lastname: White
mesh_terms: D016475:3T3 Cells; D000818:Animals; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000378:metabolism; D051379:Mice; D009687:Nuclear Proteins / Q000378:metabolism; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism*; D035463:Pol1 Transcription Initiation Complex Proteins; D011506:Proteins; D012318:RNA Polymerase I / Q000378:metabolism*; D012335:RNA, Ribosomal / Q000096:biosynthesis*; D016160:Retinoblastoma Protein / Q000235:genetics / Q000378:metabolism*; D050720:Retinoblastoma-Like Protein p107; D050717:Retinoblastoma-Like Protein p130; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D010750:Phosphoproteins; D035463:Pol1 Transcription Initiation Complex Proteins; D011506:Proteins; D012335:RNA, Ribosomal; C489485:Rbl1 protein, mouse; C493838:Rbl2 protein, mouse; D016160:Retinoblastoma Protein; D050720:Retinoblastoma-Like Protein p107; D050717:Retinoblastoma-Like Protein p130; D014157:Transcription Factors; C063844:transcription factor UBF; D012318:RNA Polymerase I