Rapid iodometric detection of Aeromonas amylase and its diagnostic significance.
Twenty-five Aeromonas hydrophila isolates from different sources (Food, 13; Clinical, 6 and Environmental, 6) were studied for the mode of production of Amylase and rapid iodometric detection of the enzyme in vitro. All twenty-five of the isolates produced the enzyme constitutively at 37 degrees C. Amylase producing ability was not dependent on the source of isolation of Aeromonas (F = 0.1069; p > 0.05). Using iodometric technique, in a microtitration tray, the enzyme was fully demonstrated in 10(40%) of the isolates within 30 min, in 22(88%) within 60 min and in all (25 or 100%) within 90 min. The rapid detection of Aeromonas amylase will, no doubt, be of great value in routine diagnostic microbiology.
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Pathology Department, Irrua Specialist Teaching Hospital, Irrua, Edo State, Nigeria.
D016980:Aeromonas hydrophila / Q000201:enzymology* / Q000302:isolation & purification; D000681:Amylases / Q000378:metabolism*; D016905:Gram-Negative Bacterial Infections / Q000175:diagnosis* / Q000382:microbiology; D006801:Humans; D013997:Time Factors
D000681:Amylases