title: Inhibition of the binding of enterotoxigenic Escherichia coli Pb176 to human intestinal epithelial cell line HCT-8 by an extracellular protein fraction containing BIF of Bifidobacterium longum SBT2928: suggestive evidence of blocking of the binding receptor gangliotetraosylceramide on the cell surface.
abstract: The extracellular protein fraction (P100V) containing the protein BIF produced by Bifidobacterium longum SBT2928 (BL2928), which inhibits the binding of enterotoxigenic Escherichia coli Pb176 (ETEC) to the glycolipid binding receptor gangliotetraosylceramide (GA1) also inhibited the binding of ETEC to the human intestinal epithelial cell line HCT-8 (ATCC CCL 244) in a dose-dependent manner. ETEC-binding inhibitory experiments using crude colonization factor antigen (CFA)-II prepared from ETEC, rabbit anti-GA1 antiserum, medium containing GA1 and media containing lectins, as the binding-inhibitors, suggest that the interaction between the CFA-II antigen present on the cell surface of ETEC and GA1 expressed on HCT-8 cells plays a significant role in the adherence between them. It is strongly suggested that the P100V fraction works as a blocker for the ETEC receptor GA1 on HCT-8 cells.
authors.affiliation: Microbiology Group, Technology and Research Institute, Snow Brand Milk Products Co., Ltd., Kawagoe, Saitama, Japan. ht4s-fjwr@asahi-net.or.jp
authors.forename: S
authors.initials: S
authors.lastname: Fujiwara
authors.forename: H
authors.initials: H
authors.lastname: Hashiba
authors.forename: T
authors.initials: T
authors.lastname: Hirota
authors.forename: J F
authors.initials: JF
authors.lastname: Forstner
mesh_terms: D001422:Bacterial Adhesion / Q000187:drug effects*; D001426:Bacterial Proteins / Q000494:pharmacology*; D001644:Bifidobacterium / Q000378:metabolism*; D001667:Binding, Competitive; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000187:drug effects* / Q000378:metabolism; D005732:Gangliosides; D006028:Glycosphingolipids / Q000037:antagonists & inhibitors*; D006801:Humans; D007413:Intestinal Mucosa / Q000166:cytology / Q000382:microbiology*; D011485:Protein Binding / Q000187:drug effects / Q000502:physiology
chemical_list: C119733:BIF protein, Bifidobacterium longum; D001426:Bacterial Proteins; C011258:G(A1) ganglioside; D005732:Gangliosides; D006028:Glycosphingolipids