The Drosophila gene twister, an orthologue of the yeast helicase SKI2, is differentially expressed during development.
We have identified and characterized a Drosophila orthologue of SKI2, which, in Saccharomyces cerevisiae, is one of the key components in the cytoplasmic 3'-5' decay of mRNA. The Drosophila orthologue (twister, tst), is expressed as two transcripts which differ in the lengths of their 3'-UTRs, with the smaller transcript being particularly abundant in 0-2 h embryos and the larger transcript reaching its highest levels in 6-8 h embryos. TST protein is expressed in two forms which are differentially expressed in adult tissues and throughout development. Differential expression of TST may modulate activity of the mRNA turnover pathway and could have a major impact on the expression of target RNAs.
Seago
J E
JE
Genetics Unit, Department of Biochemistry, University of Oxford, South Parks Road, OX1 3QU, Oxford, UK.
Chernukhin
I V
IV
Newbury
S F
SF
D000818:Animals; D004265:DNA Helicases; D004330:Drosophila / Q000196:embryology / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D029721:Drosophila Proteins; D004625:Embryo, Nonmammalian / Q000378:metabolism*; D020224:Expressed Sequence Tags; D005260:Female; D005656:Fungal Proteins / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D017344:Genes, Insect; D019476:Insect Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D008297:Male; D010053:Ovary / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D013737:Testis / Q000378:metabolism
D029721:Drosophila Proteins; D005656:Fungal Proteins; D019476:Insect Proteins; D012333:RNA, Messenger; C081929:SKI2 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D004265:DNA Helicases; C435751:tst protein, Drosophila