CRMP-2 induces axons in cultured hippocampal neurons.
In cultured hippocampal neurons, one axon and several dendrites differentiate from a common immature process. Here we found that CRMP-2/TOAD-64/Ulip2/DRP-2 (refs. 2-4) level was higher in growing axons of cultured hippocampal neurons, that overexpression of CRMP-2 in the cells led to the formation of supernumerary axons and that expression of truncated CRMP-2 mutants suppressed the formation of primary axon in a dominant-negative manner. Thus, CRMP-2 seems to be critical in axon induction in hippocampal neurons, thereby establishing and maintaining neuronal polarity.
Inagaki
N
N
Division of Signal Transduction, Nara Institute of Science and Technology, Ikoma 630-0101, Japan.
Chihara
K
K
Arimura
N
N
Ménager
C
C
Kawano
Y
Y
Matsuo
N
N
Nishimura
T
T
Amano
M
M
Kaibuchi
K
K
D000818:Animals; D002454:Cell Differentiation / Q000235:genetics*; D048429:Cell Size / Q000235:genetics*; D002478:Cells, Cultured / Q000166:cytology / Q000378:metabolism*; D003712:Dendrites / Q000378:metabolism / Q000648:ultrastructure; D019922:GAP-43 Protein / Q000378:metabolism; D020439:Growth Cones / Q000378:metabolism* / Q000648:ultrastructure; D006624:Hippocampus / Q000166:cytology / Q000196:embryology* / Q000378:metabolism; D007150:Immunohistochemistry; D036341:Intercellular Signaling Peptides and Proteins; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D009154:Mutation / Q000502:physiology; D009419:Nerve Tissue Proteins / Q000172:deficiency / Q000235:genetics*; D016704:Synapsins / Q000378:metabolism; D016708:Synaptophysin / Q000378:metabolism; D014162:Transfection; D016875:tau Proteins / Q000378:metabolism
D019922:GAP-43 Protein; D036341:Intercellular Signaling Peptides and Proteins; D008869:Microtubule-Associated Proteins; D009419:Nerve Tissue Proteins; D016704:Synapsins; D016708:Synaptophysin; C095114:collapsin response mediator protein-2; D016875:tau Proteins