title: Rapid high-performance liquid chromatographic method for the simultaneous determination of retinol, alpha-tocopherol and beta-carotene in human plasma and low-density lipoproteins.
abstract: A reversed-phase HPLC method with diode-array detection was used to simultaneously determine retinol, alpha-tocopherol and beta-carotene in human plasma and low-density lipoproteins. An aliquot of sample was de-proteinized with ethanol containing beta-tocopherol acetate as internal standard, and the analytes were extracted twice with hexane. The solvent was evaporated to dryness under a stream of nitrogen and the residue was redissolved in methanol to be injected directly into the HPLC system. A multiple solvent system based on methanol, butanol and water at a flow-rate of 2 ml/min and held at 45 degrees C provided clear separation of these compounds in only 8 min. The method showed good linearity, precision and accuracy for all compounds. Owing to its simplicity, this method may be useful in routine clinical and epidemiological work.
authors.affiliation: Dpt. Nutricío i Bromatologia, Centre de Referència en Tecnologia dels Aliments, Facultat Fe Farmácia, Universitat de Barcelona, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Gimeno
authors.forename: A I
authors.initials: AI
authors.lastname: Castellote
authors.forename: R M
authors.initials: RM
authors.lastname: Lamuela-Raventós
authors.forename: M C
authors.initials: MC
authors.lastname: de la Torre-Boronat
authors.forename: M C
authors.initials: MC
authors.lastname: López-Sabater
mesh_terms: D000328:Adult; D000368:Aged; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D008077:Lipoproteins, LDL / Q000737:chemistry*; D008297:Male; D008875:Middle Aged; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014801:Vitamin A / Q000032:analysis / Q000097:blood*; D024502:alpha-Tocopherol / Q000032:analysis / Q000097:blood*; D019207:beta Carotene / Q000032:analysis / Q000097:blood*
chemical_list: D008077:Lipoproteins, LDL; D019207:beta Carotene; D014801:Vitamin A; D024502:alpha-Tocopherol