Tripterygium wilfordii saponins and interleukin-10 prevent induction of experimental autoimmune thyroiditis by dendritic cells.
OBJECTIVE
To study the roles of Tripterygium wilfordii saponins (TII) and interleukin-10 (IL-10) on dendritic cells (DC)-induced experimental autoimmune thyroiditis (EAT).


METHODS
We used mice as autoimmune thyroiditis model animals and divided them into 4 groups, namely DC group, TII group, IL-10 group, and control group. The level of thyroglobulin (Tg) antibody was assayed by ELISA. TNF beta production in the cultured supernatants and nitric oxide (NO) in the serum were measured by biological activation assay and Griess reaction, respectively. Tg-stimulated proliferation of lymphocytes was detected with MTT incorporation assay. The histopathological analysis of thyroid was carried out.


RESULTS
Tg-pulsed DC were able to induce EAT with increase in the concentration of TgAb in serum and lymphocytes infiltration in thyroid. After treatment with TII or IL-10, DC could not induce EAT with lower levels of TgAb and no lymphocyte infiltration. The concentration of NO in serum, TNF beta activation, and the proliferation of lymphocytes in response to thyroglobulin in TII or IL-10 group were lower than those in DC group.


CONCLUSIONS
TII and IL-10 are able to strongly inhibit the ability of DC to induce experimental autoimmune thyroiditis.
Wang
S J
SJ
Department of Immunology, Zhenjiang Medical College, Zhenjiang 212001, China. shengjwang@263.net
Xu
H X
HX
Liu
G Z
GZ
D000818:Animals; D003713:Dendritic Cells; D005260:Female; D007166:Immunosuppressive Agents / Q000494:pharmacology; D016753:Interleukin-10 / Q000494:pharmacology*; D051379:Mice; D008808:Mice, Inbred CBA; D009569:Nitric Oxide / Q000097:blood; D012503:Saponins / Q000302:isolation & purification / Q000494:pharmacology*; D013967:Thyroiditis, Autoimmune / Q000097:blood / Q000209:etiology / Q000517:prevention & control*; D029757:Tripterygium / Q000737:chemistry*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D007166:Immunosuppressive Agents; D012503:Saponins; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D009569:Nitric Oxide