Comparison of planar chromatographic methods (TLC, OPLC, AMD) applied to essential oils of wild thyme and seven chemotypes of thyme.
Essential oils analysis is more often realized by gas chromatography. However, thin-layer chromatography (TLC) remains the reference for Pharmacopoeia. Nevertheless classical TLC has its own limitations but it is always a good technique because it is simple, rapid and less expensive. Actually the reproducibility, the separation quality and the possibility to obtain good and reproducible quantitative determinations have been improved significantly with automated sample applicator, scanner densitometers and two new chromatographic planar methods: the optimum performance laminar chromatography (OPLC) and automated multiple development (AMD). In this work, we show and compare the performance of these methods and TLC through a study of seven thyme chemotypes and wild thyme essential oil.
Pothier
J
J
Laboratoire de Pharmacognosie, Faculté de Pharmacie Philippe Maupas, Tours, France. pothier@univ-tours.fr
Galand
N
N
El Ouali
M
M
Viel
C
C
D001322:Autoanalysis; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D007202:Indicators and Reagents; D009822:Oils, Volatile / Q000032:analysis*; D012996:Solutions; D046930:Thymus Plant / Q000737:chemistry* / Q000235:genetics
D007202:Indicators and Reagents; D009822:Oils, Volatile; D012996:Solutions