title: Bactericidal and cytotoxic effects of sodium hypochlorite and sodium dichloroisocyanurate solutions in vitro.
abstract: The antimicrobial and cytotoxic effects of sodium hypochlorite (NaOCl) and sodium dichloroisocyanurate (NaDCC) were evaluated and compared in vitro. The minimal inhibitory concentration and minimal bactericidal concentration of NaOCl and NaDCC were tested for Streptococcus sobrinus, Streptococcus salivarius, Enterococcus faecalis, and Streptococcus mutans. The cytotoxic effect was assessed by using human fibroblast tissue culture. Survival rate was assessed by a protein determination method. Results showed that the minimal inhibitory concentration and minimal bactericidal concentration values of NaOCl and NaDCC for the tested bacteria were in a similar range. NaDCC in concentrations higher than 0.02%, and NaOCl in concentrations higher than 0.01% were lethal to fibroblasts. In conclusion it seems that both agents were very effective in killing bacteria, and their cytotoxicity to fibroblasts in tissue culture was similar.
authors.affiliation: Department of Endodontics, The Hebrew University-Hadassah, Faculty of Dental Medicine, Jerusalem, Israel.
authors.forename: I
authors.initials: I
authors.lastname: Heling
authors.forename: I
authors.initials: I
authors.lastname: Rotstein
authors.forename: T
authors.initials: T
authors.lastname: Dinur
authors.forename: Y
authors.initials: Y
authors.lastname: Szwec-Levine
authors.forename: D
authors.initials: D
authors.lastname: Steinberg
mesh_terms: D000891:Anti-Infective Agents, Local / Q000494:pharmacology* / Q000633:toxicity; D002470:Cell Survival; D002713:Chlorine / Q000494:pharmacology* / Q000633:toxicity; D046508:Culture Techniques; D004202:Disinfectants / Q000494:pharmacology* / Q000633:toxicity; D013293:Enterococcus faecalis / Q000187:drug effects; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D008826:Microbial Sensitivity Tests; D012867:Skin / Q000166:cytology / Q000187:drug effects; D012973:Sodium Hypochlorite / Q000494:pharmacology* / Q000633:toxicity; D013291:Streptococcus / Q000187:drug effects; D013295:Streptococcus mutans / Q000187:drug effects; D016986:Streptococcus sobrinus / Q000187:drug effects; D014227:Triazines / Q000494:pharmacology* / Q000633:toxicity
chemical_list: D000891:Anti-Infective Agents, Local; D004202:Disinfectants; D014227:Triazines; D002713:Chlorine; D012973:Sodium Hypochlorite; C011765:troclosene