Application of the membrane filter technique to bromodeoxyuridine immunochemistry for exfoliative cytology.
The membrane filter technique for smear specimens of tumors in bromodeoxyuridine (BrdU) immunochemistry is described. The staining results of Raji cells processed using the filter technique was compared with that obtained by the conventional cytospin method. Although the BrdU mean labeling index (LI) for in cytospin specimens was almost the same as the LI in membrane filter specimens, filter specimens showed excellent staining and less cell destruction compared with those processed by cytospin. Small amounts of tumor specimens such as squamous cell carcinoma and polymorphous low-grade adenocarcinoma also were processed using the membrane filter appliance. For squamous cell carcinoma, the LI for the filter specimens was 5.36+/-0.38 and that of the paraffin sections was 5.56+/-0.38. The membrane filter technique provided relatively undamaged specimens for exfoliative cytology and will be useful for immunohistochemical evaluation of tumor cells and for routine, noninvasive cytological screening.
Sekine
J
J
Second Department of Oral and Maxillofacial Surgery, Nagasaki University School of Dentistry, Japan. georges@net.nagasaki-u.ac.jp
Irie
A
A
Sano
K
K
Hideshima
K
K
Uehara
M
M
Inokuchi
T
T
D000230:Adenocarcinoma / Q000473:pathology; D001973:Bromodeoxyuridine; D002294:Carcinoma, Squamous Cell / Q000473:pathology; D003584:Cytological Techniques / Q000379:methods*; D006801:Humans; D007120:Immunochemistry / Q000379:methods; D007573:Jaw Neoplasms / Q000473:pathology; D008567:Membranes, Artificial; D009062:Mouth Neoplasms / Q000473:pathology; D013194:Staining and Labeling / Q000379:methods*; D014407:Tumor Cells, Cultured
D008567:Membranes, Artificial; D001973:Bromodeoxyuridine