Tumour cell uptake G2-M accumulation and cytotoxicity of NAMI-A on TS/A adenocarcinoma cells.
The ruthenium(III) complex imidazolium trans-imidazoledimethylsulfoxide-tetrachlororuthenate (NAMI-A) was tested on TS/A adenocarcinoma cells to evaluate the relationship between cell uptake, cell cycle arrest and cytotoxicity. The in vitro challenge of TS/A cells with 10(-4) M NAMI-A for 15 minutes to 4 hours showed a partial reduction of cell growth only after 4 hour exposure. In the same experimental conditions NAMI-A caused the increase of cells in G2-M cell cycle phase directly proportional on the length of treatment, and the ruthenium uptake by tumour cells, measured by flameless atomic absorption spectroscopy, that increases up to 2 hours of treatment and then reaches a plateau. The arrest of cell cycle in the pre-mitotic G2-M phase was transient and completely reversed by 48 hours after treatment. This study showed that the effect of NAMI-A on the cell cycle of TS/A cells is not strictly related to NAMI-A uptake as is the effect on tumour cell proliferation.
Bergamo
A
A
Foundation Callerio-Onlus, Biological Research Institutes of Trieste, Italy.
Zorzet
S
S
Cocchietto
M
M
Carotenuto
M E
ME
Magnarin
M
M
Sava
G
G
D000230:Adenocarcinoma / Q000188:drug therapy*; D000970:Antineoplastic Agents / Q000494:pharmacology* / Q000633:toxicity*; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division; D004121:Dimethyl Sulfoxide / Q000031:analogs & derivatives / Q000493:pharmacokinetics* / Q000633:toxicity*; D005434:Flow Cytometry; D016195:G2 Phase; D008938:Mitosis; D009942:Organometallic Compounds / Q000493:pharmacokinetics* / Q000633:toxicity*; D012428:Ruthenium / Q000494:pharmacology; D017975:Ruthenium Compounds; D013054:Spectrophotometry, Atomic; D013997:Time Factors; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D009942:Organometallic Compounds; D017975:Ruthenium Compounds; C113661:imidazolium-bis(imidazole)dimethylsulfoxideimidazotetrachlororuthenate(III); D012428:Ruthenium; D004121:Dimethyl Sulfoxide