ZMPP2, a novel type-2C protein phosphatase from maize.
A cDNA clone was selected as a candidate for the catalytic subunit of phospho-pyruvate dehydrogenase phosphatase (PDP) by screening a Zea mays expressed sequence tag database with the bovine PDP deduced amino acid sequence. Both strands of the cDNA were completely sequenced. The maize clone contains an open reading frame of 1098 base pairs that encodes a polypeptide of 40 127 Da, ZMPP2. The deduced amino acid sequence of ZMPP2 contains the five PP2C signature domains, as does PDP. However, the expression pattern of ZMPP2, determined by reverse transcriptase-polymerase chain reaction, was different from those of the maize pyruvate dehydrogenase E1 alpha subunit and pyruvate dehydrogenase kinase. Additionally, the predicted subcellular location of ZMPP2 is cytoplasmic, while the pyruvate dehydrogenase complex, regulated by reversible phosphorylation, is mitochondrial. Thus, ZMPP2 is a PP2C-type protein phosphatase related to but distinct from PDP.
Broz
A K
AK
Department of Biochemistry, University of Missouri, Columbia, Missouri 65211, USA.
Thelen
J J
JJ
Muszynski
M G
MG
Miernyk
J A
JA
Randall
D D
DD
D000818:Animals; D003593:Cytoplasm / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics; D018744:DNA, Plant; D008969:Molecular Sequence Data; D010455:Peptides / Q000235:genetics; D010749:Phosphoprotein Phosphatases / Q000235:genetics*; D054648:Protein Phosphatase 2; D000071636:Protein Phosphatase 2C; D018749:RNA, Plant; D029701:Saccharomyces cerevisiae Proteins; D003313:Zea mays / Q000201:enzymology*
D018076:DNA, Complementary; D018744:DNA, Plant; D010455:Peptides; D018749:RNA, Plant; D029701:Saccharomyces cerevisiae Proteins; C087358:PTC1 protein, S cerevisiae; D010749:Phosphoprotein Phosphatases; D054648:Protein Phosphatase 2; D000071636:Protein Phosphatase 2C