[Culture of chondrocytes using allogenous acellular cartilaginous matrix].
OBJECTIVE
To observe the effect of chondrocyte culture with natural ECM made from allogenous acellular cartilage.


METHODS
The allogenous acellular cartilaginous matrix was used as a scaffold in chondrocyte culture in vitro, comparing with the acellular perichondrium. The growth and regeneration of the chondrocytes in vitro were observed.


RESULTS
The chondrocytes seeded on the acellular cartilaginous matrix grew well while the chondrocytes on the acellular perichondrium matrix grew poorly.


CONCLUSIONS
The surface condition of the scaffold has a notable influence on the growth of the cells. The allogenous acellular cartilaginous matrix may provide a suitable condition for chondrocytes, and it has the possibility to be developed as a natural scaffold for tissue engineering.
Luan
J
J
Plastic Surgery Hospital, Chinese Academy of Medical Sciences, Beijing 100041.
Cai
Z
Z
Yang
P
P
D000818:Animals; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology*; D018920:Coculture Techniques; D004425:Ear Cartilage / Q000166:cytology*; D005109:Extracellular Matrix; D011817:Rabbits; D016378:Tissue Transplantation / Q000379:methods