title: Comparison of virulence of Legionella longbeachae strains in guinea pigs and U937 macrophage-like cells.
abstract: A guinea pig model of experimental legionellosis was established for assessment of virulence of isolates of Legionella longbeachae. The results showed that there were distinct virulence groupings of L. longbeachae serogroup 1 strains based on the severity of disease produced in this model. Statistical analysis of the animal model data suggests that Australian isolates of L. longbeachae may be inherently more virulent than non-Australian strains. Infection studies performed with U937 cells were consistent with the animal model studies and showed that isolates of this species were capable of multiplying within these phagocytic cells. Electron microscopy studies of infected lung tissue were also undertaken to determine the intracellular nature of L. longbeachae serogroup 1 infection. The data showed that phagosomes containing virulent L. longbeachae serogroup 1 appeared bloated, contained cellular debris and had an apparent rim of ribosomes while those containing avirulent L. longbeachae serogroup 1 were compact, clear and smooth.
authors.affiliation: Infectious Diseases Laboratories, Institute of Medical and Veterinary Science, Adelaide, South Australia 5000, Australia. robyn.doyle@imvs.sa.gov.au
authors.forename: R M
authors.initials: RM
authors.lastname: Doyle
authors.forename: N P
authors.initials: NP
authors.lastname: Cianciotto
authors.forename: S
authors.initials: S
authors.lastname: Banvi
authors.forename: P A
authors.initials: PA
authors.lastname: Manning
authors.forename: M W
authors.initials: MW
authors.lastname: Heuzenroeder
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D006168:Guinea Pigs; D006801:Humans; D007875:Legionella / Q000145:classification / Q000472:pathogenicity*; D007876:Legionellosis / Q000382:microbiology* / Q000473:pathology; D008168:Lung / Q000382:microbiology / Q000648:ultrastructure; D008264:Macrophages / Q000382:microbiology*; D008854:Microscopy, Electron; D010588:Phagosomes / Q000382:microbiology / Q000648:ultrastructure; D020298:U937 Cells; D014774:Virulence