title: Distribution of cytochrome P4501A (CYP1A) in the tissues of Baltic ringed and grey seals.
abstract: Information about the expression of CYP1A in wildlife species is essential for understanding the impact of organochlorine exposure on the health status of an exposed population. Therefore, we aimed at characterising a putative CYP1A enzyme expression in both hepatic and extrahepatic tissues of ringed and grey seals from the Baltic Sea and from less polluted waters. The cellular localisation of CYP1A was identified using a monoclonal antibody against scup P4501A1 (MAb 1-12-3). Immunohistochemical staining showed the highest level of CYP1A expression in liver hepatocytes, and the second highest level in the endothelial cells of capillaries and larger blood vessels in the liver and other organs. The most frequent and strongest staining was found in Baltic ringed seals. Although CYP1A-positive staining was observed in only a few tissues in the other seal populations, it was more intense in Baltic grey seals than in Canadian grey seals. The CYP1A enzyme activity, expressed as ethoxyresorufin O-deethylation (EROD), followed a similar tissue distribution and geographical pattern as the immunohistochemistry with clearly elevated EROD activities in most tissues of both Baltic seal populations. Immunochemical characterisation by immunoblotting confirmed the presence and elevation pattern of a putative CYP1A protein in ringed and grey seals, supporting our findings using other methods. The evenly distributed elevation of CYP1A expression among most of the tissues examined indicates that Baltic seals are exposed to CYP1A inducing agents affecting the whole body. This may result in an increased or decreased toxic potential of foreign substances, which may ultimately determine the biological effects of the contaminants.
authors.affiliation: Department of Pharmacology and Toxicology, University of Oulu, Finland.
authors.forename: O M
authors.initials: OM
authors.lastname: Hyyti
authors.forename: M
authors.initials: M
authors.lastname: Nyman
authors.forename: M L
authors.initials: ML
authors.lastname: Willis
authors.forename: H
authors.initials: H
authors.lastname: Raunio
authors.forename: O
authors.initials: O
authors.lastname: Pelkonen
mesh_terms: D000818:Animals; D000835:Animals, Wild; D015415:Biomarkers / Q000032:analysis; D019363:Cytochrome P-450 CYP1A1 / Q000032:analysis* / Q000096:biosynthesis; D004781:Environmental Exposure; D005260:Female; D005786:Gene Expression Regulation; D006304:Health Status; D006843:Hydrocarbons, Chlorinated; D007150:Immunohistochemistry; D007306:Insecticides / Q000009:adverse effects*; D008099:Liver / Q000201:enzymology; D008297:Male; D046048:Seals, Earless / Q000502:physiology*; D014018:Tissue Distribution
chemical_list: D015415:Biomarkers; D006843:Hydrocarbons, Chlorinated; D007306:Insecticides; D019363:Cytochrome P-450 CYP1A1