Developmental expression of the mitochondrial pyruvate dehydrogenase complex in pea (Pisum sativum) seedlings.
In order to better understand control of the mitochondrial pyruvate dehydrogenase complex (PDC), total catalytic activity was determined during development of the primary leaves of pea (Pisum sativum L.) seedlings, as well as in each leaf pair of 21-day-old plants. Activity of the PDC in clarified homogenates was highest in the youngest organs and then dropped dramatically as the leaves matured and became photosynthetically competent. As leaves began to senesce, total PDC activity dropped to zero. Steady-state mRNA levels were determined using E1 and E3 cDNA probes. The overall pattern of transcript abundance matched the pattern observed for total PDC activity; transcript levels for E1alpha and E1beta approached zero during senescence. Levels of the E1alpha, E1beta, E2 and E3 subunits of the PDC were analyzed in the same samples, using specific antibodies. Quantitation of the immunoblotting results throughout this developmental series showed a pattern in parallel with that of catalytic activity and mRNA levels, although the relative changes in subunit protein levels were not as extreme as the changes in activity. The exception to the global pattern was that of the E3 subunit: lipoamide dehydrogenase. Expression of this enzyme was highest in mature, fully expanded leaves, which were active in photosynthesis and photorespiration, reflecting the additional role of E3 as a component of glycine decarboxylase.
Luethy
Michael H.
MH
Department of Biochemistry and the Interdisciplinary Plant Group, University of Missouri, Columbia, MO 65211, USA; USDA, Agricultural Research Service, Plant Genetics Research Unit, Columbia, MO 65211, USA; Present address: Monsanto, Mystic Location, 62 Maritime Drive, Mystic, CT 06355-1958, USA; Present address: Department of Pediatric Hematology/Oncology, University of Chicago Children's Hospital, 5841 South Maryland Ave., MC4060, Chicago, IL 60637, USA; Present address: PhycoGen, Inc., 4 Moulton Street, Suite 440, Portland, ME 04101-5013, USA.
Gemel
Joanna
J
Johnston
Mark L.
ML
Mooney
Brian P.
BP
Miernyk
Jan A.
JA
Randall
Douglas D.
DD