title: Canarypox vaccines induce antigen-specific human gammadelta T cells capable of interferon-gamma production.
abstract: Induction of human gammadelta T cells was investigated in subjects who were vaccinated with live recombinant canarypox virus expressing human immunodeficiency virus (HIV) proteins or soluble MN rgp120. Both canarypox and rgp120 induced antigen-specific lymphoproliferative and interferon (IFN)-gamma responses. However, only canarypox vaccination induced increased gammadelta T cell responses detectable after secondary in vitro expansion (P<.02). These enhanced gammadelta T cell responses were specific for canarypox but not HIV antigens. Canarypox-specific gammadelta T cells were predominantly Vgamma9(+) and produced intracellular and secreted IFN-gamma. gammadelta T cell lines generated from canarypox vaccinees responded to canarypox antigens but not to mycobacterial antigens shown previously to induce bacille Calmette-Guérin-specific gammadelta T cells. Furthermore, canarypox vaccinations were associated with significantly higher NK cell expansions (P=.02). Increased IFN-gamma production by gammadelta T and NK cells could enhance the induction of protective type 1 memory immunity. Thus, stimulation of gammadelta T cells might be an important feature of live vaccines.
authors.affiliation: Division of Infectious Diseases and Immunology, Department of Internal Medicine, Saint Louis University Health Sciences Center, St. Louis, Missouri, USA. shewangizawwo@hotmail.com
authors.forename: S
authors.initials: S
authors.lastname: Worku
authors.forename: G J
authors.initials: GJ
authors.lastname: Gorse
authors.forename: R B
authors.initials: RB
authors.lastname: Belshe
authors.forename: D F
authors.initials: DF
authors.lastname: Hoft
mesh_terms: D016915:AIDS Vaccines / Q000235:genetics / Q000276:immunology*; D000941:Antigens / Q000276:immunology*; D018150:Avipoxvirus / Q000235:genetics / Q000276:immunology*; D015699:HIV Envelope Protein gp120 / Q000235:genetics / Q000276:immunology; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis*; D007694:Killer Cells, Natural; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000276:immunology; D008213:Lymphocyte Activation; D009169:Mycobacterium tuberculosis / Q000276:immunology; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000378:metabolism*; D011994:Recombinant Proteins / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D014611:Vaccination
chemical_list: D016915:AIDS Vaccines; D000941:Antigens; D015699:HIV Envelope Protein gp120; C122180:MNrgp120 vaccine; D016692:Receptors, Antigen, T-Cell, gamma-delta; D011994:Recombinant Proteins; D007371:Interferon-gamma