title: Detection of antibodies to U.S. isolates of avian pneumovirus by a recombinant nucleocapsid protein-based sandwich enzyme-linked immunosorbent assay.
abstract: The nucleocapsid (N) protein of subgroup C (United States-specific) avian pneumovirus (APV/US) was expressed in Escherichia coli, and antibodies to the recombinant N protein were shown to specifically recognize the approximately 47-kDa N protein of APV/US by Western immunoblot analysis. The recombinant APV/US N protein was used in a sandwich-capture enzyme-linked immunosorbent assay (ELISA), and the resulting assay was found to be more sensitive and specific than the routine indirect ELISA for the detection of APV/US antibodies in turkey sera.
authors.affiliation: Department of Veterinary Diagnostic Medicine, College of Veterinary Medicine, University of Minnesota, St. Paul, Minnesota 55108, USA.
authors.forename: B R
authors.initials: BR
authors.lastname: Gulati
authors.forename: S
authors.initials: S
authors.lastname: Munir
authors.forename: D P
authors.initials: DP
authors.lastname: Patnayak
authors.forename: S M
authors.initials: SM
authors.lastname: Goyal
authors.forename: V
authors.initials: V
authors.lastname: Kapur
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D019590:Nucleocapsid Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D018111:Pneumovirus / Q000276:immunology*; D018186:Pneumovirus Infections / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D011201:Poultry Diseases / Q000276:immunology / Q000821:virology*; D011817:Rabbits; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D014422:Turkeys; D014481:United States
chemical_list: D000914:Antibodies, Viral; D019590:Nucleocapsid Proteins; D011994:Recombinant Proteins