Functional genomics and the discovery of new drug targets.
Functional genomics can be defined as the search for the physiological role of a gene for which only its primary sequence is known. One example of a successful functional genomics adventure is the search for the natural ligands of orphan G protein-coupled receptors (GPCRs). GPCRs are proteins containing 7 hydrophobic domains that are the recognition sites of neurotransmitters and neuropeptides. Although many of these have been shown to interact with known natural ligands, several bind ligands that have not been thus far isolated. These are the so-called "orphan" GPCRs. As an example of functional genomics, an "orphan receptor strategy" has been developed to identify the natural ligands of orphan GPCRs. We describe that the application of this strategy has already led to the identification of 4 new neuropeptides and report on what has been learned about these neuropeptides. We finally discuss the importance of the application of the orphan receptor strategy to the development of novel drugs.
Civelli
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Department of Pharmacology and Department of Developmental and Cell Biology, University of California, Irvine, California, USA. ocivelli@uci.edu
Nothacker
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D000818:Animals; D015195:Drug Design; D019204:GTP-Binding Proteins; D023281:Genomics; D006801:Humans; D008024:Ligands; D009479:Neuropeptides / Q000235:genetics; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics / Q000502:physiology*; D017981:Receptors, Neurotransmitter / Q000235:genetics / Q000502:physiology*
D008024:Ligands; D009479:Neuropeptides; D018160:Receptors, Cytoplasmic and Nuclear; D017981:Receptors, Neurotransmitter; D019204:GTP-Binding Proteins