Enhanced Ca(2+) influx with mossy fiber stimulation in hippocampal CA3 neurons of spontaneously epileptic rats.
This study was performed to determine whether the intracellular Ca(2+) concentration ([Ca(2+)](i)) is increased in hippocampal CA3 neurons of spontaneously epileptic rats (SER) which show both absence-like and convulsive seizures using hippocampal slices loaded with Calcium Green-1 when a weak single stimulation is given to the mossy fiber. [Ca(2+)](i) in the CA3 area was significantly increased after a single stimulus to mossy fibers in SER, while no changes were detected in normal Wistar rats. These findings suggest the existence of an abnormality in the Ca(2+) channel in the SER CA3 region and that this is probably responsible for epileptic seizures.
Amano
T
T
Department of Pharmacology, Hiroshima University School of Medicine, Hiroshima 734-8551, Japan.
Amano
H
H
Matsubayashi
H
H
Ishihara
K
K
Serikawa
T
T
Sasa
M
M
D000200:Action Potentials / Q000502:physiology; D000818:Animals; D002118:Calcium / Q000378:metabolism; D015220:Calcium Channels / Q000378:metabolism*; D020013:Calcium Signaling / Q000502:physiology*; D004558:Electric Stimulation; D004827:Epilepsy / Q000378:metabolism* / Q000503:physiopathology; D005260:Female; D006624:Hippocampus / Q000378:metabolism* / Q000503:physiopathology; D007424:Intracellular Fluid / Q000378:metabolism; D008297:Male; D019599:Mossy Fibers, Hippocampal / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D051381:Rats; D011922:Rats, Mutant Strains
D015220:Calcium Channels; D002118:Calcium