title: Determination of amlodipine in human plasma by high-performance liquid chromatography with fluorescence detection.
abstract: A sensitive and specific HPLC method has been developed for the assay of amlodipine in human plasma. The assay involves derivatization with 4-chloro-7-nitrobenzofurazan (NBD-Cl), solid-phase extraction on a silica column and isocratic reversed-phase chromatography with fluorescence detection. Nortriptyline hydrochloride was used as an internal standard. The assay was linear over the concentration range of 0.25-18.00 ng/ml. Both of the within-day and day-to-day reproducibility and accuracy were less than 11.80% and 12.00%, respectively. The plasma profile following a single administration of 10 mg amlodipine to a healthy volunteer was presented.
authors.affiliation: University of Istanbul, Faculty of Pharmacy, Department of Analytical Chemistry, Turkey.
authors.forename: S
authors.initials: S
authors.lastname: Tatar
authors.forename: S
authors.initials: S
authors.lastname: Atmaca
mesh_terms: D017311:Amlodipine / Q000097:blood*; D002121:Calcium Channel Blockers / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence
chemical_list: D002121:Calcium Channel Blockers; D017311:Amlodipine