Estrogen receptor beta dominates in baboon carotid after endothelial denudation injury.
Increasing evidence, mainly from rodents, suggests that the predominant estrogen receptor (ER) in arteries is the newly-described ERbeta. We have investigated the expression of the two ERs in baboon carotid artery before and after denudation injury. Prior to denudation, both full length receptors were detected in semiquantitative RT-PCR; in addition two ERalpha but no ERbeta splicing variants were found. After denudation, ERbeta mRNA increased five-fold and declined, whereas ERalpha mRNA expression remained low. Prior to and after denudation, two ERalpha-specific antibodies showed no reaction with the vessel wall. Instead, two affinity purified antisera to ERbeta demonstrated a weak but distinct reaction over vascular smooth muscle cells with predenudation specimens, escalating post-denudation and declining thereafter. The results suggest that selective targeting to ERbeta should be attempted when designing estrogen-based vasculoprotective drug therapies devoid of uterotrophic side effects.
Aavik
E
E
Transplantation Laboratory and Rational Drug Design Program, University of Helsinki Central Hospital, P.O. Box 21 (Haartmaninkatu 3), FIN 00014, Helsinki, Finland.
du Toit
D
D
Myburgh
E
E
Frösen
J
J
Hayry
P
P
D017398:Alternative Splicing; D000818:Animals; D002339:Carotid Arteries / Q000166:cytology / Q000378:metabolism*; D002404:Catheterization / Q000009:adverse effects; D004730:Endothelium, Vascular / Q000293:injuries*; D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D005260:Female; D007106:Immune Sera / Q000494:pharmacology; D007150:Immunohistochemistry; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects; D010215:Papio / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D011960:Receptors, Estrogen / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D001743:Urinary Bladder / Q000737:chemistry / Q000166:cytology; D014599:Uterus / Q000737:chemistry / Q000166:cytology
D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D007106:Immune Sera; D012333:RNA, Messenger; D011960:Receptors, Estrogen