[Relationship between the bone marrow cell proliferation and the prognosis in childhood acute lymphoblastic leukemia].
OBJECTIVE
To study the expression of proliferative antigens in leukemic cells and the relationship between the cell proliferation activity and the prognosis.


METHODS
The labeling index (LI) of CD71, Ki-67 and PCNA in normal and leukemic cells were measured by flow cytometry.


RESULTS
In normal children, the LI of CD71, Ki-67 and PCNA was (32.18 +/- 16.66)%, (4.82 +/- 9.27)% and (19.69 +/- 25.11)%, respectively, while in ALL children, which was (33.66 +/- 21.52)%, (32.14 +/- 23.59)% and (47.46 +/- 30.96)%, respectively. The PCNA LI of leukemia cells was (47.46 +/- 30.96)% and (27.28 +/- 12.51)% when patients was at presentation and remitted for 3 years, respectively, and was (52.59 +/- 32.00)% and at presentation in 28 CCR children and and (26.94 +/- 14.48)% in 7 high-risk group ALL children (F = 8.877, P < 0.01), respectively.


CONCLUSIONS
The proliferation, LI of leukemic cells was higher than that of normal cells, and reduced to normal level after treatment. The higher PCNA proliferation of untreated cells was a favourable marker for prognosis.
Zheng
H
H
Beijing Children's Hospital, Beijing 100045.
Zhao
X
X
Geng
L
L
D000293:Adolescent; D015703:Antigens, CD / Q000378:metabolism; D000944:Antigens, Differentiation, B-Lymphocyte / Q000378:metabolism; D002455:Cell Division; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007223:Infant; D019394:Ki-67 Antigen / Q000378:metabolism; D008297:Male; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000276:immunology / Q000473:pathology*; D011379:Prognosis; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism*; D011990:Receptors, Transferrin
D015703:Antigens, CD; D000944:Antigens, Differentiation, B-Lymphocyte; C068126:CD71 antigen; D019394:Ki-67 Antigen; D018809:Proliferating Cell Nuclear Antigen; D011990:Receptors, Transferrin