title: First field trial of a transmissible recombinant vaccine against myxomatosis and rabbit hemorrhagic disease.
abstract: As a novel approach for immunisation of wild rabbits, we have recently developed a transmissible vaccine against myxomatosis and rabbit hemorrhagic disease (RHD) based on a recombinant myxoma virus (MV) expressing the RHDV capsid protein [J. Virol. 74 (2000) 1114]. The efficacy and safety of the vaccine have been extensively evaluated under laboratory conditions. In this study, we report the first limited field trial of the candidate vaccine that was undertaken in an island of 34 Has containing a population of around 300 rabbits. Following administration by the subcutaneous route to 76 rabbits, the vaccine induced specific antibody responses against both myxomatosis and RHDV in all the inoculated rabbits. Furthermore, the recombinant virus exhibited a limited horizontal transmission capacity, promoting seroconversion of around 50% of the uninoculated rabbit population. No evidence of undesirable effects due to the recombinant virus field release was detected.
authors.affiliation: Centro de Investigación en Sanidad Animal (CISA-INIA), Valdeolmos, 28130, Madrid, Spain.
authors.forename: J M
authors.initials: JM
authors.lastname: Torres
authors.forename: C
authors.initials: C
authors.lastname: Sánchez
authors.forename: M A
authors.initials: MA
authors.lastname: Ramírez
authors.forename: M
authors.initials: M
authors.lastname: Morales
authors.forename: J
authors.initials: J
authors.lastname: Bárcena
authors.forename: J
authors.initials: J
authors.lastname: Ferrer
authors.forename: E
authors.initials: E
authors.lastname: Espuña
authors.forename: A
authors.initials: A
authors.lastname: Pagès-Manté
authors.forename: J M
authors.initials: JM
authors.lastname: Sánchez-Vizcaíno
mesh_terms: D000818:Animals; D000835:Animals, Wild; D017250:Caliciviridae Infections / Q000517:prevention & control* / Q000662:veterinary*; D002460:Cell Line; D018562:Disease Transmission, Infectious / Q000517:prevention & control / Q000662:veterinary*; D017929:Hemorrhagic Disease Virus, Rabbit / Q000276:immunology*; D009233:Myxoma virus / Q000276:immunology*; D009234:Myxomatosis, Infectious / Q000517:prevention & control* / Q000635:transmission*; D011817:Rabbits; D014611:Vaccination / Q000009:adverse effects / Q000379:methods; D017778:Vaccines, Combined / Q000627:therapeutic use; D014614:Vaccines, Synthetic / Q000009:adverse effects / Q000627:therapeutic use; D014765:Viral Vaccines / Q000009:adverse effects / Q000627:therapeutic use*
chemical_list: D017778:Vaccines, Combined; D014614:Vaccines, Synthetic; D014765:Viral Vaccines