Molecular cloning and characterization of canine metalloproteinase-9 gene promoter.
This paper describes the cloning and characterization of the canine matrix metalloproteinase-9 (MMP-9) gene promoter. The 5' untranslated region was obtained by genome walking upstream of the canine MMP-9 translation start site using canine genomic DNA as template. A DNA fragment of 1894 bp was isolated and on analysis demonstrated regions of sequence homology with the MMP-9 promoter sequences already determined for other species. In general, conserved regions correlated with DNA binding motifs such as a TATA-like box, AP-1 sites, GC boxes and a nuclear factor-kappaB binding domain. The DNA promoter fragment was sufficient to drive basal expression of a luciferase reporter gene in Madin Darby canine kidney (MDCK) cells and to a lesser extent in feline embryonic fibroblast (FEA) cells. Activity of the promoter was enhanced by the treatment of transfected MDCK cells with phorbol 12-myristate 13-acetate but no effect was observed in the FEA cells. Promoter deletion studies revealed that regions of promoter were necessary for induction of reporter gene expression.
Campbell
S E
SE
Department of Clinical Studies, Faculty of Veterinary Medicine, University of Glasgow, Glasgow, UK. s.campbell@vet.gla.ac.uk
Nasir
L
L
Argyle
D J
DJ
Bennett
D
D
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D018387:Codon, Initiator; D004285:Dogs / Q000235:genetics*; D015870:Gene Expression; D020780:Matrix Metalloproteinase 9 / Q000235:genetics*; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic
D018387:Codon, Initiator; D020780:Matrix Metalloproteinase 9