title: Quantification of daunorubicin and daunorubicinol in plasma by capillary electrophoresis.
abstract: Capillary electrophoresis (CE) with laser-induced fluorescence detection was applied to quantify daunorubicin and daunorubicinol in plasma. Separation was carried out in a 47 cm x 50 microm I.D. fused-silica capillary, with a running buffer. pH 5 containing 60 microM spermine and 70% acetonitrile. Sample preparation was done either by protein precipitation with acetonitrile or by liquid-liquid extraction. The assay can be applied in a concentration range from 40 mg/l down to 2 microg/l for daunorubicin and from 1 mg/l to 2 microg/l for daunorubicinol. Precision and accuracy were between 2.9 and 14.5% (n=6) on 1 day and between 1.0 and 14.7% from day to day (n=6) for both analytes. Thus, the CE method enables precise and accurate quantification of daunorubicin and daunorubicinol in small sample volumes over a wide concentration range.
authors.affiliation: Institut für Pharmazeutische Chemie, Universität Münster, Germany. hempege@uni-muenster.de
authors.forename: G
authors.initials: G
authors.lastname: Hempel
authors.forename: P
authors.initials: P
authors.lastname: Schulze-Westhoff
authors.forename: S
authors.initials: S
authors.lastname: Flege
authors.forename: J
authors.initials: J
authors.lastname: Boos
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000097:blood*; D003630:Daunorubicin / Q000031:analogs & derivatives* / Q000097:blood*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D015203:Reproducibility of Results
chemical_list: D000903:Antibiotics, Antineoplastic; C000847:daunorubicinol; D003630:Daunorubicin