Design of RNA-binding proteins and ligands.
The rapidly expanding database of RNA structures and protein complexes is beginning to lead to the successful design of specific RNA-binding molecules. Recent combinatorial and structure-based approaches have utilized known nucleic-acid-binding scaffolds from both proteins and small molecules to display a relatively small set of functional groups often used in protein--RNA recognition. Several studies have shown that the tethering of multiple binding modules can enhance RNA-binding affinity and specificity, a strategy also commonly used in DNA recognition.
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Department of Biochemistry and Biophysics, University of California San Francisco, 94143-0448, USA. frankel@cgl.ucsf.edu
Calabro
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Frankel
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D000818:Animals; D020650:Combinatorial Chemistry Techniques; D008024:Ligands; D008958:Models, Molecular; D009414:Nerve Growth Factors; D019151:Peptide Library; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry*; D016601:RNA-Binding Proteins / Q000737:chemistry* / Q000378:metabolism*; D013329:Structure-Activity Relationship; D015534:Trans-Activators / Q000737:chemistry*; D016335:Zinc Fingers
D008024:Ligands; C099695:MANF protein, human; D009414:Nerve Growth Factors; D019151:Peptide Library; D011506:Proteins; D016601:RNA-Binding Proteins; D015534:Trans-Activators