title: Structures of cytochrome c-549 and cytochrome c6 from the cyanobacterium Arthrospira maxima.
abstract: Cytochrome c(6) and cytochrome c-549 are small (89 and 130 amino acids, respectively) monoheme cytochromes that function in photosynthesis. They appear to have descended relatively recently from the same ancestral gene but have diverged to carry out very different functional roles, underscored by the large difference between their midpoint potentials of nearly 600 mV. We have determined the X-ray crystal structures of both proteins isolated from the cyanobacterium Arthrospira maxima. The two structures are remarkably similar, superimposing on backbone atoms with an rmsd of 0.7 A. Comparison of the two structures suggests that differences in solvent exposure of the heme and the electrostatic environment of the heme propionates, as well as in heme iron ligation, are the main determinants of midpoint potential in the two proteins. In addition, the crystal packing of both A. maxima cytochrome c-549 and cytochrome c(6) suggests that the proteins oligomerize. Finally, the cytochrome c-549 dimer we observe can be readily fit into the recently described model of cyanobacterial photosystem II.
authors.affiliation: Molecular Biology Institute, University of California, Los Angeles, Box 951570, Los Angeles, California 90095-1570, USA.
authors.forename: M R
authors.initials: MR
authors.lastname: Sawaya
authors.forename: D W
authors.initials: DW
authors.lastname: Krogmann
authors.forename: A
authors.initials: A
authors.lastname: Serag
authors.forename: K K
authors.initials: KK
authors.lastname: Ho
authors.forename: T O
authors.initials: TO
authors.lastname: Yeates
authors.forename: C A
authors.initials: CA
authors.lastname: Kerfeld
mesh_terms: D000595:Amino Acid Sequence; D003198:Computer Simulation; D017124:Conserved Sequence; D003460:Crystallization; D018360:Crystallography, X-Ray; D000458:Cyanobacteria / Q000201:enzymology*; D003574:Cytochrome c Group / Q000737:chemistry*; D003580:Cytochromes / Q000737:chemistry*; D045348:Cytochromes f; D019281:Dimerization; D006418:Heme / Q000737:chemistry; D006860:Hydrogen Bonding; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011199:Potentiometry; D017510:Protein Folding
chemical_list: D003574:Cytochrome c Group; D003580:Cytochromes; D006418:Heme; D045348:Cytochromes f; C031732:cytochrome C-550