title: Cytokines in cervicovaginal washing fluid from patients with cervical neoplasia.
abstract: Human papillomavirus (HPV) infections play an important role in the development of cervical neoplasia. To get to a better understanding of the role of cytokines in the development of these neoplasias, we analysed the presence of various cytokines in cervicovaginal washings of healthy volunteers (n=22), cervical intraepithelial neoplasia (CIN) patients (n=63) and cervical cancer patients (n=33). IL-12p40, IL-10, TGF-beta1, TNF-alpha and IL-1beta levels were significantly higher in patients with cervical cancer than in controls and CIN patients. The levels of IFN-gamma were not different. Our data demonstrate alterations in the local cervical immune environment in cervical cancer patients. This could have important consequences for the further development of immune modulating therapies and vaccination strategies.
authors.affiliation: Department of Obstetrics and Gynecology, Academic Medical Center, Amsterdam, The Netherlands.
authors.forename: M Y
authors.initials: MY
authors.lastname: Tjiong
authors.forename: N
authors.initials: N
authors.lastname: van der Vange
authors.forename: J S
authors.initials: JS
authors.lastname: ter Schegget
authors.forename: M P
authors.initials: MP
authors.lastname: Burger
authors.forename: F W
authors.initials: FW
authors.lastname: ten Kate
authors.forename: T A
authors.initials: TA
authors.lastname: Out
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D002584:Cervix Uteri / Q000378:metabolism* / Q000821:virology; D016207:Cytokines / Q000096:biosynthesis* / Q000378:metabolism; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis; D007375:Interleukin-1 / Q000096:biosynthesis; D016753:Interleukin-10 / Q000096:biosynthesis; D018664:Interleukin-12 / Q000096:biosynthesis; D008875:Middle Aged; D027383:Papillomaviridae / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000096:biosynthesis; D053773:Transforming Growth Factor beta1; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis; D002583:Uterine Cervical Neoplasms / Q000378:metabolism* / Q000821:virology; D014621:Vagina / Q000378:metabolism* / Q000821:virology
chemical_list: D016207:Cytokines; D007375:Interleukin-1; C508960:TGFB1 protein, human; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D018664:Interleukin-12; D007371:Interferon-gamma