In vivo detection of tobacco mosaic virus-induced local and systemic oxidative burst by electron paramagnetic resonance spectroscopy.
This is the first demonstration that tobacco mosaic virus-induced oxidative stress in a necrotic host plant is signalled by an elevated level of monodehydroascorbate (MDA) radicals detected by electron paramagnetic resonance spectroscopy. Furthermore, systemic acquired resistance induced in remote leaves of Xanthi-nc tobacco is also associated with stimulated MDA signals indicative of a microoxidative burst.
Fodor
J
J
Plant Protection Institute, Hungarian Academy of Sciences, Box 102, H-1525 Budapest, Hungary. jfod@nki.hu
Hideg
E
E
Kecskés
A
A
Király
Z
Z
D003683:Dehydroascorbic Acid / Q000031:analogs & derivatives* / Q000378:metabolism; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods; D004594:Electrophysiology; D006790:Host-Parasite Interactions / Q000276:immunology / Q000502:physiology; D008027:Light; D018515:Plant Leaves / Q000502:physiology / Q000821:virology; D016897:Respiratory Burst / Q000502:physiology*; D015398:Signal Transduction; D014026:Nicotiana / Q000502:physiology / Q000821:virology*; D014027:Tobacco Mosaic Virus / Q000302:isolation & purification / Q000472:pathogenicity
C000820:semidehydroascorbic acid; D003683:Dehydroascorbic Acid