[Melatonin decreases the release of amino acid transmitters in hypoxic rat cortical slice].
To study the effect of melatonin on changes in amino acid release following hypoxic stimulation, a RP-HPLC fluorometric method was used to measure the content of amino acids in the culture medium of rat brain slices. Hypoxic condition was instituted by a gas of 91.6% N2 and 8.4% O2. The results showed that the contents of amino acids, including aspartate, glutamate, glutamine, glycine, taurine and GABA, in the medium were significantly increased to 240.4%, 334.3%, 200.6%, 210.4%, 168.6% and 263.9%, respectively (P < 0.01 vs control) by 30 min hypoxic incubation. Melatonin can significantly reduce the increase of aspartate and glutamate, to 55.1% and 57.0% of the hypoxic group.
Gu
J
J
National Key Laboratory of Medical Neurobiology, Department of Neurobiology, Shanghai Medical University, Shanghai 200032.
Li
X J
XJ
Sun
F Y
FY
D000818:Animals; D001224:Aspartic Acid / Q000378:metabolism; D015687:Cell Hypoxia; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000378:metabolism*; D018846:Excitatory Amino Acids / Q000378:metabolism*; D018698:Glutamic Acid / Q000378:metabolism; D008297:Male; D008550:Melatonin / Q000494:pharmacology*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
D018846:Excitatory Amino Acids; D001224:Aspartic Acid; D018698:Glutamic Acid; D008550:Melatonin