title: Characterization of classical swine fever virus associated with defective interfering particles containing a cytopathogenic subgenomic RNA isolated from wild boar.
abstract: Classical swine fever virus (CSFV) strain WB82, isolated from a wild boar in 1982, induced a distinct cytopathic effect (CPE) in primary swine testicle cell culture and in most of the porcine cell lines. This strain of CSFV was found to be composed of two biotypes. cytopathogenic (cp) CSFV, as a minor population, and noncytopathogenic (noncp) CSFV, as a major population. The noncp CSFV (designated strain WB82/E+) was obtained by biological cloning, and it showed the exaltation of Newcastle disease virus phenomenon. In Northern blot analysis and RT-PCR assay, CSFV RNA with a subgenomic (sg) length was detected in addition to full-length viral RNA only in the cells in which a CPE had been revealed. These RNAs represent the genomes of typical defective interfering (DI) particles because of the strict dependence on a complementing helper virus and interference with replication of the helper virus. The sg RNA, which exhibits the genomes of the DI particles, lacked the nucleotides of the viral genomic region from Npro to NS2 (4764 bases). When extracted sg RNA was transfected to the cells infected with the WB82/E+ strain, a distinct CPE was observed. Interestingly, the CPE was observed in cells infected with other heterologous noncp CSFV ALD and GPE- strains by sg RNA transfection. The results suggested that these noncp CSFVs act as helper viruses for the replication of sg RNA (DI particles). It was also shown that the cytopathogenicity of strain WB82 is caused by apoptosis.
authors.affiliation: Assay Division I, National Veterinary Assay Laboratory, Kokubunji, Tokyo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Aoki
authors.forename: K
authors.initials: K
authors.lastname: Ishikawa
authors.forename: Y
authors.initials: Y
authors.lastname: Sakoda
authors.forename: H
authors.initials: H
authors.lastname: Sekiguchi
authors.forename: M
authors.initials: M
authors.lastname: Kodama
authors.forename: S
authors.initials: S
authors.lastname: Suzuki
authors.forename: A
authors.initials: A
authors.lastname: Fukusho
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology; D001483:Base Sequence; D001681:Biological Assay; D015152:Blotting, Northern; D002478:Cells, Cultured; D006691:Classical Swine Fever / Q000821:virology*; D006692:Classical Swine Fever Virus / Q000737:chemistry / Q000235:genetics* / Q000472:pathogenicity; D053938:DNA Fragmentation; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D003673:Defective Viruses / Q000737:chemistry / Q000235:genetics* / Q000472:pathogenicity; D020287:In Situ Nick-End Labeling / Q000662:veterinary; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D012689:Sequence Homology, Nucleic Acid; D013552:Swine; D014162:Transfection / Q000662:veterinary; D014774:Virulence / Q000235:genetics
chemical_list: D004279:DNA, Viral; D012367:RNA, Viral