title: [Human preadipocyte culture and the establishment of hyperplasia and hypertrophy model].
abstract: OBJECTIVE
  To better understand hyperplasia and hypertrophy properties of human adipose tissue.
  
  
  METHODS
  Using an "adhesion-ceiling" culture strategy, fibroblast-like cells from adult pure adipose granules were successfully cultured.
  
  
  RESULTS
  The cells were highly homogeneous, highly proliferative and with a high differentiation rate. Their dynamic morphological changes, growth curve, glycerophosphate dehydrogenase pattern, oil red O staining, and reaction to insulin and dexamethasone all verified their preadipocyte identity. Under controlled conditions, the preadipocytes replayed their in vivo hyperplasia and hypertrophy process.
  
  
  CONCLUSIONS
  In mature human adipose tissue there exist functionally active fractions that can be modified. This study laid the basis for further probing into obesity and fat autotransplantation.
authors.affiliation: Department of Plastic and Reconstructive Surgery, Second Military Medical University, Shanghai 200003.
authors.forename: X
authors.initials: X
authors.lastname: Zhu
authors.forename: Q
authors.initials: Q
authors.lastname: He
authors.forename: Z
authors.initials: Z
authors.lastname: Lin
mesh_terms: D017667:Adipocytes / Q000166:cytology* / Q000201:enzymology; D000273:Adipose Tissue / Q000166:cytology*; D002454:Cell Differentiation; D002455:Cell Division; D002478:Cells, Cultured; D005993:Glycerolphosphate Dehydrogenase / Q000378:metabolism; D006801:Humans; D006965:Hyperplasia; D006984:Hypertrophy
chemical_list: D005993:Glycerolphosphate Dehydrogenase