title: Comparison of endothelial function evaluated by strain gauge plethysmography and brachial artery ultrasound.
abstract: Strain gauge plethysmography and brachial artery ultrasound are widely used to study endothelial function. No data on correlation between these two procedures are reported. The present study compared these two methods and investigated the correlation between vasodilation and brachial wall shear stress. In six healthy subjects and ten patients with hypertension or obesity, strain gauge plethysmography was performed in resting conditions and after infusion of 7.5,15 and 30 microg/min of acetylcholine, and brachial artery ultrasound in resting conditions and after 5 min hand ischemia. Wall shear stress was calculated as: blood viscosity x blood velocity/internal diameter. Forearm blood flow following acetylcholine infusion increased more in healthy subjects than in patients with hypertension or obesity. In addition, brachial artery dilated more in the former group. Change in brachial artery diameter correlated with change in forearm blood flow, calculated as area under the curve of acetylcholine infusion (r=0.739, P<0.001). Wall shear stress was higher in healthy subjects (67.8+/-20.0 dynes/cm(2)) than in patients with either hypertension or obesity (39.2+/-16.7, P<0.001), and correlated with variations of diameter (r=0.796, P<0.0002), and marginally of blood flow (r=0.516, P<0.05). The present findings demonstrate that there is a high correlation between endothelial function evaluated by strain gauge plethysmography and brachial artery ultrasound. Wall shear stress correlates with brachial artery diameter change following hand ischemia, and marginally with blood flow change following acetylcholine infusion.
authors.affiliation: Dipartimento di Medicina Sperimentale e Clinica G. Salvatore, Institute of Internal Medicine, Magna Graecia University, 88100 Catanzaro, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Irace
authors.forename: R
authors.initials: R
authors.lastname: Ceravolo
authors.forename: L
authors.initials: L
authors.lastname: Notarangelo
authors.forename: A
authors.initials: A
authors.lastname: Crescenzo
authors.forename: G
authors.initials: G
authors.lastname: Ventura
authors.forename: O
authors.initials: O
authors.lastname: Tamburrini
authors.forename: F
authors.initials: F
authors.lastname: Perticone
authors.forename: A
authors.initials: A
authors.lastname: Gnasso
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000328:Adult; D001696:Biomechanical Phenomena; D001783:Blood Flow Velocity; D001809:Blood Viscosity; D001916:Brachial Artery / Q000000981:diagnostic imaging*; D004730:Endothelium, Vascular / Q000502:physiology* / Q000503:physiopathology; D005542:Forearm / Q000098:blood supply; D006801:Humans; D006973:Hypertension / Q000503:physiopathology; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000503:physiopathology; D010991:Plethysmography; D018608:Ultrasonography, Doppler; D014664:Vasodilation / Q000187:drug effects; D014665:Vasodilator Agents / Q000494:pharmacology
chemical_list: D014665:Vasodilator Agents; D000109:Acetylcholine