[Determination of mephenytoin enantiomers in human urine using chiral capillary chromatography and its application in metabolism polymorphism study].
The determination of mephenytoin enantiomers in human urine using chiral capillary chromatography is described. MP in urine was extracted with using dichloroethane and the organic layer was washed with acidic and basic aqueous solution. The organic phase was evaporated to dryness under nitrogen on water bath (55 degrees C). The residue was dissolved in 10 microL ethyl acetate and 1 microL was injected into the GC. MP enantiomers was separated on a Chirasil-Val SFOT column with nitrogen as carrier gas and FID as detector. The linear relationship was obtained over the concentration range of 115-690 micrograms/L (r = 0.9913 for S-MP and r = 0.9934 for R-MP) with a detection limit of 60 ng MP enantiomers/mL urine. The recoveries were 74.41% for S-MP and 73.78% for R-MP. The relative standard deviation within day and between days were less than 6.5%. The method was used to study the metabolism polymorphism of 32 volunteers. The S/R ratio was calculated to express interindividual variation in metabolism, in which, the S/R ratio of 5 subjects was more than 0.95, as the poor metabolizers and that of the others was less than 0.8, as the extensive metabolizers.
Yao
T
T
Department of Pharmacy, Zhejiang Medical University, Hangzhou, 310031.
Zeng
S
S
Ruan
H
H
Chen
S
S
D000927:Anticonvulsants / Q000652:urine*; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods; D006801:Humans; D007536:Isomerism; D008617:Mephenytoin / Q000652:urine*; D011110:Polymorphism, Genetic
D000927:Anticonvulsants; D008617:Mephenytoin