An optimized protocol for first strand cDNA synthesis from laser capture microdissected tissue.
Boylan
S
S
Department of Ophthalmology, University of California Davis, Davis, California, USA.
Honda
S
S
Hjelmeland
L M
LM
Handa
J T
JT
D018076:DNA, Complementary / Q000096:biosynthesis*; D004210:Dissection / Q000379:methods*; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D007834:Lasers; D008268:Macular Degeneration / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D008875:Middle Aged; D010857:Pigment Epithelium of Eye / Q000378:metabolism / Q000473:pathology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity
D018076:DNA, Complementary; D012333:RNA, Messenger; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases