[Flavonoid drugs as antioxidant in intraocular irrigating solution].
OBJECTIVE
To study the protective effect for vitrectomy of intraocular irrigating solutions containing different concentrations of two antioxidants: Meralop and Activadone and compare them with saline solutions and BSS plus.


METHODS
Seventy-five pigmented rabbits were divided into 8 groups: I. Control (n=8); II. Gas-vitrectomy (n=15); III. Vitrectomy + saline solution (n=6); IV. Vitrectomy + BSS plus (n=9); V. Vitrectomy + Activadone 90 mg/l (n=8); VI. Vitrectomy + Activadone 180 mg/l (n=9); VII. Vitrectomy + Meralop 16 mg/l (n=10); VIII. Vitrectomy + Meralop 64 mg/l (n=10). Antioxidant activity in lens and retina was measured evaluating the levels of lipoperoxides malondyaldehide (MDA) and 4-hydroxynonenal (4-HNE) by spectrophotometry.


RESULTS
MDA + 4-HNE concentrations in lens and retina of each group (mean and standard deviation in nmol/g) were: Group I= 3.806DE1.043, 38.787DE10.404. Group II= 4.353DE3.626, 41.554DE18.520. Group III= 297.306DE103.73, 156.69DE6.82. Group IV= 29.39DE15.38, 112.02DE50.12. Group V= 42.31DE20.82, 55.44DE42.21. Group VI= 27.66DE9.29, 181.061DE79.39. Group VII= 134.93DE133.52, 76.89DE36.02. Group VIII= 112.91DE60.30, 79.97DE38.005.


CONCLUSIONS
All intraocular irrigation solutions containing antioxidant substances produced lower MDA + 4-HNE levels than saline solution. In the retina, Activadone 90 mg/l produces less MDA concentration than BSS plus.
Manzanas
L
L
Instituto Universitario de Oftalmobiología Aplicada (IOBA), Grupo de Retina, Universidad de Valladolid, España.
Rodríguez
E
E
Marcos
M A
MA
Cordero
M Y
MY
Pastor
J C
JC
D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use*; D005419:Flavonoids / Q000627:therapeutic use*; D009883:Ophthalmic Solutions / Q000627:therapeutic use*; D011817:Rabbits; D007507:Therapeutic Irrigation; D014821:Vitrectomy
D000975:Antioxidants; D005419:Flavonoids; D009883:Ophthalmic Solutions