Repopulating activities of human cord blood cells separated by a stem cell collection filter in NOD/SCID mice: a comparative study of filter method and HES method.
BACKGROUND
Volume reduction and removal of RBCs are essential for cost-efficient cord blood (CB) banking. It has previously been shown that a newly developed device, a stem cell-collection filter (SCCF), can reduce the CB volume and remove RBCs efficiently, giving high recovery rates for CD34+ cells, colony-forming cells, and long-term culture-initiating cells with short operation time. The aim of this study was to compare the quality of CB cells separated by SCCF and HES by analyzing repopulation in NOD/SCID mice.


METHODS
A total of 1 x 10(6) or 5 x 10(6) nucleated cells derived from SCCF- or HES-separated, cryopreserved, thawed, and washed CB were transplanted into NOD/SCID mice. Eight weeks after transplantation, bone marrow cells of the recipient mice were examined by flow cytometry and hematopoietic progenitor assay for the engraftment of human cells.


RESULTS
Mice given human CB cells, separated by SCCF, showed degrees of engraftment similar to those in mice given HES-separated CB cells. There was no significant difference in the lymphohematopoietic reconstitution pattern in the two groups of mice.


CONCLUSIONS
SCCF processing does not appear to reduce the number of repopulating cells in NOD/SCID mice or alter the number of HPCs. It is now shown that these cells can be captured by SCCF and removed, and that they will engraft.
Tokushima
Y
Y
Division of Cell Processing, The Institute of Medical Science, University of Tokyo, Tokyo, Japan.
Sasayama
N
N
Takahashi
T A
TA
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000502:physiology; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods* / Q000592:standards; D002498:Centrifugation; D015925:Cryopreservation; D005312:Fetal Blood / Q000166:cytology*; D005374:Filtration; D006085:Graft Survival; D006410:Hematopoiesis; D006801:Humans; D006895:Hydroxyethyl Starch Derivatives; D007937:Leukapheresis / Q000295:instrumentation / Q000379:methods / Q000592:standards; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology*; D014183:Transplantation, Heterologous
D006895:Hydroxyethyl Starch Derivatives