[The rapid effects of steroids on glycine uptake in neuroblastoma cell strain SK-N-SH cells].
In the present study, glycine uptake in SK-N-SH cells was determined with liquid scintillation technique, and the rapid effects of steroids on glycine uptake in SK-N-SH cells were investigated. The results were as follows. High-affinity glycine uptake in SK-N-SH cells was dependent on Na+ and Cl-. Corticosterone (CORT), progesterone (P) and dexamethasone (DEX) had rapid effects on the glycine uptake. Since estradiol (E2) and deoxycorticosterone (DOC) had no effects, it was suggested that the rapid effects of steroids were specific. The rapid effects of CORT were concentration-dependent in a range of 10(-9)-10(-6) mol/L. The rapid effects were not affected by the inhibitor of protein synthesis and persisted even when CORT was conjugated with bovine serum album, but attenuated when Ca2+ was absent in the external medium. The results suggest that the steroid effect on glycine uptake in SK-N-SH cells was nongenomicly mediated.
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G
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Neuroscience Research Institute, Second Military Medical University, Shanghai 200433.
Chen
Y Z
YZ
D003345:Corticosterone / Q000494:pharmacology*; D003907:Dexamethasone / Q000494:pharmacology; D005998:Glycine / Q000378:metabolism*; D006801:Humans; D009447:Neuroblastoma / Q000378:metabolism / Q000473:pathology*; D011374:Progesterone / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D011374:Progesterone; D003907:Dexamethasone; D005998:Glycine; D003345:Corticosterone