Effect of histamine H3-receptor antagonist clobenpropit on spatial memory of radial maze performance in rats.
OBJECTIVE
The effect of histamine H3-receptor antagonist, clobenpropit (VUF9153) on spatial memory deficits induced by scopolamine was investigated in rats.


METHODS
Eight-Arm radial maze performance was used to measure spatial memory in rats, and the brain regions were subsequently dissected and histamine contents were determined by HPLC.


RESULTS
Intracerebroventricular (icv) injection of clobenpropit (50 micrograms) ameliorated memory impairment induced by scopolamine in both parameters of radial maze performance. The amelioration induced by clobenpropit was antagonized by an H3-agonist, (R)-alpha-methylhistamine (5-10 micrograms). alpha-Fluoromethylhistidine (10, 20 micrograms), a histidine decarboxylase inhibitor, also effectively reversed clobenpropit-induced ameliorating effects.


CONCLUSIONS
The brain histamine H3-receptor antagonists are highly related to the spatial memory, and this action may be due to cholinergic neurons.
Chen
Z
Z
Department of Pharmacology, School of Medicine, Zhejiang University, Hangzhou 310031, China. chenzfl@hotmail.com
D000818:Animals; D001921:Brain / Q000378:metabolism; D006632:Histamine / Q000378:metabolism*; D006633:Histamine Antagonists / Q000494:pharmacology*; D007093:Imidazoles / Q000494:pharmacology*; D007276:Injections, Intraventricular; D008297:Male; D018782:Maze Learning / Q000187:drug effects*; D008569:Memory Disorders / Q000139:chemically induced / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012601:Scopolamine; D013890:Thiourea / Q000031:analogs & derivatives / Q000494:pharmacology*
D006633:Histamine Antagonists; D007093:Imidazoles; D006632:Histamine; D012601:Scopolamine; D013890:Thiourea; C086108:clobenpropit