title: Hematopoietic capability of CD34+ cord blood cells: a comparison with CD34+ adult bone marrow cells.
abstract: The characteristics of hematopoietic progenitor and stem cell (HPC/HSC) populations in mammals vary according to their ontogenic stage. In humans, HPC/HSCs from umbilical cord blood (CB) are increasingly used as an alternative to HPC/HSCs from adult bone marrow (BM) for the treatment of various hematologic disorders. How the hematopoietic activity of progenitor and stem cells in CB differs from that in adult BM remains unclear, however. We compared CD34+ cells, a hematopoietic cell population, in CB with those in adult BM using phenotypic subpopulations analyzed by flow cytometry, the colony-forming activity in methylcellulose clonal cultures, and the repopulating ability of these cells in NOD/Shi-scid (NOD/SCID) mice. Although the proportion of CD34+ cells was higher in adult BM than in CB mononuclear cells, the more immature subpopulations, CD34+ CD33- and CD34+ CD38- cells, were present in higher proportions in CD34+ CB cells. Clonal culture assay showed that more multipotential progenitors were present in CD34+ CB cells. When transplanted into NOD/SCID mice. CD34+ adult BM cells could not reconstitute human hematopoiesis in recipient BM, but CD34+ CB cells achieved a high level of engraftment, indicating that CD34+ CB cells possess a greater repopulating ability. These results demonstrated that human hematopoiesis changes with development from fetus to adult. Furthermore, CD34+ CB cells contained a greater number of primitive hematopoietic cells, including HSCs, than did adult BM, suggesting the usefulness of CD34+ CB cells not only as a graft for therapeutic HSC transplantation but also as a target cell population for ex vivo expansion of transplantable HSCs and for gene transfer in gene therapy.
authors.affiliation: Department of Pediatric Hematology/Oncology, The Institute of Medical Science, The University of Tokyo, Tokyo.|Department of Pediatrics, Nippon Medical School, Tokyo.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Ueda
authors.affiliation: Department of Pediatrics, Hokkaido University School of Medicine, Sapporo.
authors.forename: Makoto
authors.initials: M
authors.lastname: Yoshida
authors.affiliation: Department of Pediatric Hematology/Oncology, The Institute of Medical Science, The University of Tokyo, Tokyo.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yoshino
authors.affiliation: Central Institute for Experimental Animals, Kawasaki.
authors.forename: Kimio
authors.initials: K
authors.lastname: Kobayashi
authors.affiliation: Central Institute for Experimental Animals, Kawasaki.
authors.forename: Mariko
authors.initials: M
authors.lastname: Kawahata
authors.affiliation: Department of Pediatric Hematology/Oncology, The Institute of Medical Science, The University of Tokyo, Tokyo.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Ebihara
authors.affiliation: Central Institute for Experimental Animals, Kawasaki.
authors.forename: Mamoru
authors.initials: M
authors.lastname: Ito
authors.affiliation: Department of Pediatric Hematology/Oncology, The Institute of Medical Science, The University of Tokyo, Tokyo.|Department of Hematology/Oncology, The Institute of Medical Science, The University of Tokyo, Tokyo.
authors.forename: Shigetaka
authors.initials: S
authors.lastname: Asano
authors.affiliation: Department of Pediatrics, Kyoto University, Kyoto, Japan.
authors.forename: Tatsutoshi
authors.initials: T
authors.lastname: Nakahata
authors.affiliation: Department of Pediatric Hematology/Oncology, The Institute of Medical Science, The University of Tokyo, Tokyo. tsujik@ims.u-tokyo.ac.jp.
authors.forename: Kohichiro
authors.initials: K
authors.lastname: Tsuji
mesh_terms: D000328:Adult; D000818:Animals; D018952:Antigens, CD34 / Q000032:analysis / Q000502:physiology*; D001854:Bone Marrow Cells / Q000166:cytology / Q000276:immunology / Q000502:physiology; D005312:Fetal Blood / Q000166:cytology / Q000276:immunology / Q000502:physiology*; D006085:Graft Survival; D006410:Hematopoiesis; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D014183:Transplantation, Heterologous
chemical_list: D018952:Antigens, CD34