[The determination of melatonin in health-caring medicine for specific purposes by high performance liquid chromatography-mass spectrometry].
A rapid and precise method for the determination of melatonin in health-caring medicine for specific purposes, based on high performance liquid chromatography-mass spectrometry, has been developed. The compound was identified using DAD UV spectrum and mass spectrum. Hypersil ODS column, 100 mm x 2.1 mm i.d., and mobile phase of methanol-water (70:30, V/V) were used for the assays. The quantitation was performed with external standard, and detection at 275 nm. The calibration curve of melatonin was linear in the range of 5-50 mg/L and the detection limit was ca. 0.2 ng.
Chen
W
W
National Centre of Import Food Hygiene Supervision & Inspection of P. R. China, Guangzhou, 510405.
Chen
Y
Y
Hu
G
G
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D019587:Dietary Supplements / Q000032:analysis*; D016166:Free Radical Scavengers / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D008550:Melatonin / Q000032:analysis*
D016166:Free Radical Scavengers; D008550:Melatonin