Integrons and gene cassettes in the enterobacteriaceae.
Integrons were detected in 59 of 120 (49%) urinary isolates of Enterobacteriaceae by PCR using degenerate primers targeted to conserved regions of class 1, 2, and 3 integrase genes. PCR sequencing analysis of the cassette arrays revealed a predominance of cassettes that confer resistance to the aminoglycosides and trimethoprim.
White
P A
PA
Virology Division, Department of Microbiology, SEALS, Prince of Wales Hospital, Randwick, Sydney NSW 2031, Australia. whitepa@sesahs.nsw.gov.au
McIver
C J
CJ
Rawlinson
W D
WD
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D004352:Drug Resistance, Microbial / Q000235:genetics; D004755:Enterobacteriaceae / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D006801:Humans; D019426:Integrases / Q000235:genetics*; D009713:Nucleotidyltransferases / Q000235:genetics; D016133:Polymerase Chain Reaction; D014295:Trimethoprim / Q000494:pharmacology
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D014295:Trimethoprim; D019426:Integrases; D009713:Nucleotidyltransferases; C115006:integron integrase IntI1; C022678:streptomycin 3''-adenylyltransferase