title: High-performance liquid chromatography method for the quantification of non-radiolabelled cinnamic compounds in analytes derived from human skin absorption and metabolism experiments.
abstract: An isocratic high-performance liquid chromatography method has been developed for the quantification of the skin sensitisers trans-cinnamaldehyde and trans-cinnamic alcohol, and their cinnamic metabolites. The relative standard deviations (RSDs) between the gradients of eight sets of standard curves were 2.8, 3.1 and 1.9% for cinnamic alcohol, cinnamaldehyde and cinnamic acid, respectively. Sample analytes were derived from two series of experiments: in vitro full-thickness human skin absorption and metabolism studies and metabolism studies using human skin homogenates, with non-radiolabelled cinnamic compounds. Skin absorption and metabolism experiments were performed in the absence and presence of the alcohol dehydrogenase inhibitor, pyrazole. Samples from full-thickness skin absorption studies were analysed without extraction; cinnamic compounds from within skin were extracted into methanolic solutions using newly developed methods. The intra-assay RSDs ranged from 0.17 to 2.52% for cinnamic alcohol, 0.24 to 9.14% for cinnamaldehyde and 0.26 to 6.43% for cinnamic acid. The inter-assay RSDs for cinnamic alcohol, cinnamaldehyde and cinnamic acid, respectively, as determined from n=20 HPLC runs, were 2.10, 4.16 and 2.26%.
authors.affiliation: Division of Biomedical Sciences, Imperial College School of Medicine, South Kensington, London, UK.
authors.forename: C K
authors.initials: CK
authors.lastname: Smith
authors.forename: C
authors.initials: C
authors.lastname: Cheung
authors.forename: E N
authors.initials: EN
authors.lastname: Elahi
authors.forename: S A
authors.initials: SA
authors.lastname: Hotchkiss
mesh_terms: D000171:Acrolein / Q000031:analogs & derivatives* / Q000032:analysis* / Q000493:pharmacokinetics; D000328:Adult; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D008875:Middle Aged; D020005:Propanols / Q000032:analysis* / Q000493:pharmacokinetics; D015203:Reproducibility of Results; D012869:Skin Absorption; D013056:Spectrophotometry, Ultraviolet
chemical_list: D020005:Propanols; D000171:Acrolein; C012843:cinnamaldehyde; C020722:cinnamyl alcohol