[Ciliary neurotrophic factor prevents NO mediated cytotoxicity in cultured hippocampal neurons].
The effects of ciliary neurotrophic factor on nitric oxide (NO)-induced cytotoxicity were examined in primary cultures of rat hippocampal neurons. The neurotoxicity was induced by NO donors, such as sodium nitroprusside (SNP), s-nitroso-N-acetylpenicillamine (SNAP), NOS substrate, L-arginine (L-Arg) and ionomycin (a calcium ionophore). Preincubation of the cultures with ciliary neurotrophic factor (CNTF) for 24 h promoted neuronal survival and decreased the LDH efflux and the level of NO induced by ionomycin. The protective effects of CNTF against NO cytotoxicity were concentration-dependent. The above findings suggest that CNTF promotes the survival of neurons in the hippocampus by preventing NO mediated neurotoxicity.
Chen
X Q
XQ
Department of Neurobiology, Institute of Neuroscience Research, Second Military Medical University, Shanghai 200433.
Chen
Z Y
ZY
Lu
C L
CL
He
C
C
Wang
C H
CH
Bao
X
X
D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D020934:Ciliary Neurotrophic Factor / Q000494:pharmacology*; D006624:Hippocampus / Q000378:metabolism* / Q000473:pathology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D009474:Neurons / Q000473:pathology; D018696:Neuroprotective Agents / Q000494:pharmacology*; D009569:Nitric Oxide / Q000502:physiology*; D009599:Nitroprusside; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
D020934:Ciliary Neurotrophic Factor; D018696:Neuroprotective Agents; D009599:Nitroprusside; D009569:Nitric Oxide; D007770:L-Lactate Dehydrogenase