title: Antihypertensive effects of D-polymannuronic sulfate and its related mechanisms in renovascular hypertensive rats.
abstract: OBJECTIVE
  To investigate the antihypertensive effects of D-polymannuronic sulfate (DPS), a kind of sulfated polysaccharide, and the underlying mechanisms in renovascular hypertensive rats (RHR).
  
  
  METHODS
  Used two-kidney one clip (Goldblatt, 2-K 1C) method to produce RHR model. DPS was given i.v. or ig for 5 wk with the initiation of establishment of RHR. Serum nitric oxide (NO) was determined with NO kit; plasma angiotensin II (Ang II) and endothelin-1 (ET-1) were measured by radioimmumoassays.
  
  
  RESULTS
  In acute therapeutic experiments, DPS markedly reduced systolic blood pressure (SBP) and diastolic blood pressure (DBP) dose-dependently and decreased heart rate (HR) with reduction in arterial blood pressure. In the prophylactic experiments, DPS prevented the rise in SBP and DBP in a dose-dependent manner. The hypotensive potency of DPS 50 mg/kg is comparable to that of captopril (14 mg/kg). Moreover, DPS elevated serum NO contents and lowered plasma concentrations of Ang II and ET-1.
  
  
  CONCLUSIONS
  The antihypertensive activities of DPS might be involved both in increasing the generation of nitric oxide and in decreasing the production of angiotensin II and endothelin-1 in vivo.
authors.affiliation: Department of Marine Pharmacology, Marine Drug and Food Institute, Ocean University of Qingdao, Qingdao 266003, China.
authors.forename: H B
authors.initials: HB
authors.lastname: Zhu
authors.forename: M Y
authors.initials: MY
authors.lastname: Geng
authors.forename: H S
authors.initials: HS
authors.lastname: Guan
authors.forename: J T
authors.initials: JT
authors.lastname: Zhang
mesh_terms: D000804:Angiotensin II / Q000097:blood; D000818:Animals; D000959:Antihypertensive Agents / Q000302:isolation & purification / Q000494:pharmacology*; D001794:Blood Pressure / Q000187:drug effects; D019332:Endothelin-1 / Q000097:blood; D006339:Heart Rate / Q000187:drug effects; D006603:Hexuronic Acids / Q000302:isolation & purification / Q000494:pharmacology*; D006978:Hypertension, Renovascular / Q000097:blood* / Q000503:physiopathology; D008297:Male; D009569:Nitric Oxide / Q000097:blood*; D000459:Phaeophyceae / Q000737:chemistry; D051381:Rats; D011921:Rats, Inbred WKY
chemical_list: D000959:Antihypertensive Agents; D019332:Endothelin-1; D006603:Hexuronic Acids; C437391:polymannuronic sulfate; D000804:Angiotensin II; D009569:Nitric Oxide