Analysis of arylsulfatases A and B, acid phosphatase, lactate dehydrogenase, and aspartate transaminase in chronic periapical lesions of endodontic origin.
Attempts were made to detect and measure the activities of arylsulfatases. A&B acid phosphatase, lactate dehydrogenase, and glutamate oxaloacetate transaminase (aspartate transaminase) enzymes in human chronic lesions of endodontic origin. Thirteen periapical lesions of endodontic origin and 11 noninflamed control periapical tissues were obtained. The specimens were carried to the laboratory on liquid nitrogen and kept at -70 degrees C. Samples were thawed, homogenized, and then assayed for enzyme activities. The specific activities of arylsulfatase A (nmol/hr/mg protein) were 55.0+/-10.7 (chronic lesions) vs. 3.4+/-2.2 (controls) (p < 0.01). Arylsulfatase B specific activities (nmol/hr/mg protein) were 50.3+/-6.4 (chronic lesions) vs 91.8+/-18.4 (controls). Total acid phosphatase activities (mU/mg protein) were 45.8+/-6.6 (chronic lesions) vs. 26.8+/-3.1 (controls). Lactate dehydrogenase activities (Berger-Broida units/mg protein) of the chronic periapical lesions were significantly higher than the control group (362+/-63.2) vs. (140+/-46.0) (p < 0.05). There was no significant difference between the specific activities of aspartate transaminase in chronic lesions and the control group (68.0+/-14.5) vs. (53.0+/-10.4) mU/mg protein).
Khayat
A
A
Department of Endodontics, School of Dental Medicine, Shiraz University of Medical Sciences, Iran.
Vesal
N
N
Rasti
M
M
D000135:Acid Phosphatase / Q000032:analysis*; D001219:Aspartate Aminotransferases / Q000032:analysis*; D002553:Cerebroside-Sulfatase / Q000032:analysis*; D002908:Chronic Disease; D003788:Dental Pulp Diseases / Q000150:complications; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000032:analysis*; D020051:N-Acetylgalactosamine-4-Sulfatase / Q000032:analysis*; D010483:Periapical Diseases / Q000201:enzymology*; D010486:Periapical Tissue / Q000201:enzymology; D013053:Spectrophotometry; D013223:Statistics as Topic
D007770:L-Lactate Dehydrogenase; D001219:Aspartate Aminotransferases; D000135:Acid Phosphatase; D020051:N-Acetylgalactosamine-4-Sulfatase; D002553:Cerebroside-Sulfatase