title: Restriction and modification systems of ruminal bacteria.
abstract: A high frequency of type II restriction endonuclease activities was detected in Selenomonas ruminantium but not in other rumen bacteria tested. Eight different restriction endonucleases were characterized in 17 strains coming from genetically homogeneous local population. Chromosomal DNA isolated from S. ruminantium strains was found to be refractory to cleavage by various restriction enzymes, implying the presence of methylase activities additional to those required for protection against the cellular endonucleases. The presence of Dam methylation was detected in S. ruminantium strains as well as in several other species belonging to the Sporomusa subbranch of low G + C Gram-positive bacteria (Megasphaera elsdenii, Mitsuokella multiacidus).
authors.affiliation: Institute of Animal Physiology, Slovak Academy of Sciences, 040 01 Kosice, Slovakia.
authors.forename: P
authors.initials: P
authors.lastname: Pristas
authors.forename: V
authors.initials: V
authors.lastname: Molnárová
authors.forename: P
authors.initials: P
authors.lastname: Javorský
mesh_terms: D000818:Animals; D015280:DNA Restriction-Modification Enzymes / Q000145:classification / Q000378:metabolism*; D003670:Deer; D016965:Gram-Negative Anaerobic Straight, Curved, and Helical Rods / Q000201:enzymology; D012417:Rumen / Q000382:microbiology*; D020357:Selenomonas / Q000145:classification / Q000201:enzymology*; D016985:Streptococcus bovis / Q000201:enzymology
chemical_list: D015280:DNA Restriction-Modification Enzymes