title: The multi-organ origin of interleukin-5 in the mouse.
abstract: Murine Ba/F3 cells were transfected with cDNA for the alpha-chain of the murine interleukin-5 (IL-5) receptor and cloned lines of these cells were able to proliferate in response to as little as 2.5 pg/ml of IL-5. The bioassay was demonstrated to be specific for IL-5 and was able to measure IL-5 produced in culture by organs from adult C57BL/6 and BALB/c mice. The highest levels of IL-5 were produced by lung tissue but thymus and bladder consistently produced IL-5 and more variable production was observed by the heart, spleen, muscle, bone shaft, uterus and testes. Bone marrow cells produced no detectable IL-5. Observed levels of production of IL-5 were similar when using organs from mice lacking high-affinity receptors for IL-5 and from nu/nu, RAG-1-/- and NOD/SCID mice lacking T lymphocytes. In inflammatory peritoneal exudates induced by the injection of casein plus bacteria, levels of induced IL-5 were higher if the mice lacked high-affinity receptors for IL-5. The data indicate that T lymphocytes are not the dominant cellular source of IL-5 in organ-conditioned media and that local IL-5 production can occur with a wide range of normal murine organs.
authors.affiliation: The Walter and Eliza Hall Institute of Medical Research, PO Royal Melbourne Hospital, Victoria, Australia.
authors.forename: P J
authors.initials: PJ
authors.lastname: Ryan
authors.forename: T
authors.initials: T
authors.lastname: Willson
authors.forename: W S
authors.initials: WS
authors.lastname: Alexander
authors.forename: L
authors.initials: L
authors.lastname: Di Rago
authors.forename: S
authors.initials: S
authors.lastname: Mifsud
authors.forename: D
authors.initials: D
authors.lastname: Metcalf
mesh_terms: D000818:Animals; D001681:Biological Assay; D018076:DNA, Complementary / Q000032:analysis / Q000235:genetics; D005786:Gene Expression Regulation; D015848:Interleukin-5 / Q000032:analysis* / Q000096:biosynthesis / Q000235:genetics; D051379:Mice; D009928:Organ Specificity
chemical_list: D018076:DNA, Complementary; D015848:Interleukin-5