title: The humoral immune response to recombinant nucleocapsid antigen of canine distemper virus in dogs vaccinated with attenuated distemper virus or DNA encoding the nucleocapsid of wild-type virus.
abstract: This study compared the humoral immune response against the nucleocapsid-(N) protein of canine distemper virus (CDV) of dogs vaccinated with a multivalent vaccine against parvo-, adeno-, and parainfluenza virus and leptospira combined with either the attenuated CDV Onderstepoort strain (n = 15) or an expression plasmid containing the N-gene of CDV (n = 30). The vaccinations were applied intramuscularly three times at 2-week intervals beginning at the age of 6 weeks. None of the pre-immune sera recognized the recombinant N-protein, confirming the lack of maternal antibodies at this age. Immunization with DNA vaccine for CDV resulted in positive serum N-specific IgG response. However, their IgG (and IgA) titres were lower than those of CDV-vaccinated dogs. Likewise, DNA-vaccinated dogs did not show an IgM peak. There was no increase in N-specific serum IgE titres in either group. Serum titres to the other multivalent vaccine components were similar in both groups.
authors.affiliation: Institute of Animal Neurology, Bremgartenstrasse 109a, University of Berne, 3012 Berne, Switzerland. mgriot-wenk@healthecon.com
authors.forename: M E
authors.initials: ME
authors.lastname: Griot-Wenk
authors.forename: P
authors.initials: P
authors.lastname: Cherpillod
authors.forename: A
authors.initials: A
authors.lastname: Koch
authors.forename: R
authors.initials: R
authors.lastname: Zurbriggen
authors.forename: L
authors.initials: L
authors.lastname: Bruckner
authors.forename: R
authors.initials: R
authors.lastname: Wittek
authors.forename: A
authors.initials: A
authors.lastname: Zurbriggen
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis* / Q000097:blood; D000956:Antigens, Viral / Q000097:blood; D004216:Distemper / Q000276:immunology / Q000517:prevention & control*; D004217:Distemper Virus, Canine / Q000276:immunology*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D007136:Immunoglobulins / Q000096:biosynthesis / Q000097:blood; D007273:Injections, Intramuscular / Q000662:veterinary; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D019590:Nucleocapsid Proteins / Q000235:genetics / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology; D014611:Vaccination / Q000662:veterinary; D014613:Vaccines, Attenuated; D019444:Vaccines, DNA; D014765:Viral Vaccines
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D007136:Immunoglobulins; D019590:Nucleocapsid Proteins; D011994:Recombinant Proteins; D014613:Vaccines, Attenuated; D019444:Vaccines, DNA; D014765:Viral Vaccines