title: Estimation of width of narrow molecular-weight distributions by size-exclusion chromatography with concentration and light scattering detection.
abstract: A new method for the estimation of the weight-to-number-average molecular-weight ratio, Mw/Mn of polymers with a narrow molecular-weight distribution, approximated by log-normal distribution, is proposed using size-exclusion chromatography (SEC) with concentration and light-scattering detectors. From experimental data, the Mw/Mn ratios are calculated by two procedures: one using the concentration and light-scattering elution curve for the polymer measured, and the other based on the concentration elution curve and calibration line for a wide range of molecular masses. An iteration method has been developed making the two Mw/Mn ratios converge. The method was applied to a series of narrow molecular-weight distribution polystyrene standards.
authors.affiliation: Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Prague. netopil@imc.cas.cz
authors.forename: M
authors.initials: M
authors.lastname: Netopilík
authors.forename: S
authors.initials: S
authors.lastname: Podzimek
authors.forename: P
authors.initials: P
authors.lastname: Kratochvíl
mesh_terms: D002138:Calibration; D002850:Chromatography, Gel / Q000379:methods*; D008027:Light; D008970:Molecular Weight; D012542:Scattering, Radiation