title: Dose- and time-dependent formation of biliary benzo[a]pyrene metabolites in the marine flatfish dab (Limanda limanda).
abstract: Polycyclic aromatic hydrocarbons (PAHs) are abundant pollutants, and many PAHs are carcinogenic, but only after metabolic activation. Benzo[a]pyrene (BaP) is among the most carcinogenic PAHs. The dose and time response of two enzymes involved in BaP metabolism and the amounts of BaP metabolites excreted into the bile were evaluated in an experiment with dab (Limanda limanda). Ninety dab were exposed orally to one of five doses of BaP (0, 0.08, 0.4, 2, or 10 mg/kg) and sampled at 3, 6, or 12 d after exposure. None of the doses studied caused significant induction of either microsomal ethoxyresorufin-O-deethylase (EROD). which reflects cytochrome P450 1A (CYP1A) activity, or cytosolic glutathione-S-transferase activity (GST). Concentrations of biliary BaP metabolites significantly increased with dose and significantly decreased with time after exposure. It is concluded that biliary BaP metabolites provide a much more sensitive method than EROD (CYP1A) or GST activity to monitor recent exposure to PAHs in dab.
authors.affiliation: Netherlands Institute for Sea Research, Department of Marine Biogeochemistry and Toxicology, Den Burg Texel. a.vanschanke@azu.nl
authors.forename: A
authors.initials: A
authors.lastname: van Schanke
authors.forename: F
authors.initials: F
authors.lastname: Holtz
authors.forename: J P
authors.initials: JP
authors.lastname: van der Meer
authors.forename: J P
authors.initials: JP
authors.lastname: Boon
authors.forename: F
authors.initials: F
authors.lastname: Ariese
authors.forename: G
authors.initials: G
authors.lastname: Stroomberg
authors.forename: M
authors.initials: M
authors.lastname: van den Berg
authors.forename: J M
authors.initials: JM
authors.lastname: Everaarts
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001564:Benzo(a)pyrene / Q000737:chemistry / Q000378:metabolism* / Q000493:pharmacokinetics; D001646:Bile / Q000737:chemistry; D015415:Biomarkers / Q000032:analysis; D002273:Carcinogens / Q000737:chemistry / Q000378:metabolism* / Q000493:pharmacokinetics; D019363:Cytochrome P-450 CYP1A1 / Q000032:analysis / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004781:Environmental Exposure; D004784:Environmental Monitoring; D005412:Flatfishes / Q000502:physiology*; D005982:Glutathione Transferase / Q000032:analysis / Q000494:pharmacology; D007700:Kinetics; D012680:Sensitivity and Specificity
chemical_list: D015415:Biomarkers; D002273:Carcinogens; D001564:Benzo(a)pyrene; D019363:Cytochrome P-450 CYP1A1; D005982:Glutathione Transferase