MRI of lung parenchyma in rats and mice using a gradient-echo sequence.
Signal of lung parenchymal tissue from the living rat and mouse lung was detected at 4.7 T with a good signal-to-noise ratio and motion-suppressed artifacts using a short TE gradient-echo sequence. Neither cardiac nor respiratory gating were applied, and animals respired freely during data collection. Mean T(2)* relaxation times of parenchyma in the anterior, middle and posterior regions of both lungs ranged between 403 and 657 micros and 397 and 751 micros, respectively for the rat and mouse. For the rat in the prone position, there was a gradient in T(2)* values, from the posterior to the anterior regions of both lungs. In the supine position, however, T(2)* values were larger in the posterior and in the anterior portions. For the mouse in both prone and supine positions, there was a tendential gradient in T(2)* from the anterior to the posterior portions. The robustness of the approach renders it well suited for routine applications, e.g. in pharmacological studies concerning asthma models in small rodents. The method was applied to lung inflammation models involving challenge with ovalbumin or lipopolysaccharide.
Beckmann
N
N
Core Technologies Area, Novartis Pharma AG, Analytics and Imaging Sciences Unit, CH-4002 Basel, Switzerland. nicolau.beckmann@pharma.novartis.com
Tigani
B
B
Mazzoni
L
L
Fozard
J R
JR
D000818:Animals; D001249:Asthma / Q000139:chemically induced / Q000175:diagnosis*; D008070:Lipopolysaccharides; D008168:Lung / Q000473:pathology*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D010047:Ovalbumin; D051381:Rats; D017207:Rats, Sprague-Dawley
D008070:Lipopolysaccharides; D010047:Ovalbumin