title: Determination of beta-2 microglobulin levels in plasma using a high-throughput mass spectrometric immunoassay system.
abstract: A high-throughput mass spectrometric immunoassay (MSIA) system for the analysis of proteins directly from biological fluids is reported. A 96-well-format robotic workstation equipped with antibody-derivatized affinity pipet tips was used for the parallel extraction of specific proteins from samples and subsequent deposition onto 96-well arrayed matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) targets. Interferences from nonspecifically bound proteins were minimized through choice of appropriate affinity pipet tip derivatization chemistries. Sample preparation for MALDI-TOFMS was enhanced through the use of hydrophobic/hydrophilic contrasting targets, which also presented functionalities found to promote matrix/analyte crystal growth. Automated mass spectrometry was used in the unattended acquisition of data, resulting in an analysis rate of approximately 100 samples/h (biological fluid-->data). The quantitative MSIA of beta2m levels present in human plasma samples is given as illustration.
authors.affiliation: Intrinsic Bioprobes, Inc., Tempe, Arizona 85281, USA.
authors.forename: E E
authors.initials: EE
authors.lastname: Niederkofler
authors.forename: K A
authors.initials: KA
authors.lastname: Tubbs
authors.forename: K
authors.initials: K
authors.lastname: Gruber
authors.forename: D
authors.initials: D
authors.lastname: Nedelkov
authors.forename: U A
authors.initials: UA
authors.lastname: Kiernan
authors.forename: P
authors.initials: P
authors.lastname: Williams
authors.forename: R W
authors.initials: RW
authors.lastname: Nelson
mesh_terms: D000328:Adult; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D008297:Male; D008875:Middle Aged; D015203:Reproducibility of Results; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013379:Substrate Specificity; D001613:beta 2-Microglobulin / Q000097:blood*
chemical_list: D001613:beta 2-Microglobulin