[Detection of point mutation of p53 gene in head and neck squamous cell carcinoma by non-isotopic PCR-SSCP].
Non-isotopic PCR-SSCP technique was used to detect point mutation of p53 gene in fresh tissue taken from head and neck squamous cell carcinoma. The abnormal band of the single-stranded DNA (ssDNA) was identified in 7 of 13 cases. The results showed that point mutation occurred in the related DNA fragments. The point mutation of exon 5 was found in one case and that of exon 6, 7, 8 in two respectively. These results indicated that the p53 gene mutation was closely correlated with the development of head and neck squamous cell carcinoma. Compared with the traditional isotopic PCR-SSCP technique, the non-isotopic PCR-SSCP one is a kind of simpler, safer, quicker and more effective way to detect the mutation of p53 tumor suppressor gene. It is especially useful in the screening of point mutation when handling a large number of samples.
Wang
J
J
Department of Oral & Maxillofacial Surgery, Hubei Medical University.
Huang
H
H
Li
J
J
D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D005260:Female; D016158:Genes, p53; D006801:Humans; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000235:genetics; D017354:Point Mutation; D016133:Polymerase Chain Reaction / Q000379:methods; D018807:Polymorphism, Single-Stranded Conformational; D014062:Tongue Neoplasms / Q000235:genetics*