Study of interaction of mannan-BSA neoglycoconjugates with Concanavalin A.
Neoglycoconjugates prepared by synthesis of oxidized mannans from Saccharomyces cerevisiae with bovine serum albumin were studied for interaction with Concanavalin A. The mannan-bovine serum albumin neoglycoproteins, different in degree of mannan oxidation used for synthesis and its content in conjugates were prepared. The interaction of these glycoconjugates with Concanavalin A by using precipitation method was investigated. The conjugates prepared at high weight ratio mannan: protein (4:1) involved 47-65% of saccharides and formed by precipitation with Concanavalin A aggregates with low content of protein. The obtained results showed that conjugates with lower content of mannan (up to 30%) are more efficacious for their aggregation with Concanavalin A than the conjugates with high content of mannan.
Mislovicová
D
D
Institute of Chemistry Slovak Academy of Sciences, Bratislava, Slovak Republic.
Masárová
J
J
Gemeiner
P
P
D011232:Chemical Precipitation; D003208:Concanavalin A / Q000737:chemistry / Q000378:metabolism*; D006023:Glycoproteins / Q000032:analysis / Q000138:chemical synthesis / Q000378:metabolism*; D008351:Mannans / Q000032:analysis / Q000737:chemistry*; D008358:Mannose / Q000138:chemical synthesis / Q000737:chemistry; D008970:Molecular Weight; D010084:Oxidation-Reduction; D011485:Protein Binding; D012441:Saccharomyces cerevisiae / Q000737:chemistry; D012709:Serum Albumin / Q000138:chemical synthesis / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000032:analysis / Q000737:chemistry*
D006023:Glycoproteins; D008351:Mannans; D012709:Serum Albumin; C023840:mannose-bovine serum albumin conjugate; D003208:Concanavalin A; D012710:Serum Albumin, Bovine; D008358:Mannose