Identification of a new imprinted gene, Rian, on mouse chromosome 12 by fluorescent differential display screening.
Systematic screening of differentially expressed genes among androgenetic, parthenogenetic, and normal embryos by means of fluorescent differential display revealed five imprinted genes. One of them, named Rian, was expressed exclusively from the maternal allele and was closely linked to an imprinted gene, Meg3(Gtl2), mapped to the distal end of chromosome 12. The Rian transcript did not have any apparent open reading frame, and its transcript was exclusively localized to the nucleus.
Hatada
I
I
Gene Research Center, Gunma University, Showa-machi, Maebashi, Gunma 371-8511, Japan. ihatada@showa.gunma-u.ac.jp
Morita
S
S
Obata
Y
Y
Sotomaru
Y
Y
Shimoda
M
M
Kono
T
T
D000818:Animals; D001483:Base Sequence; D004622:Embryo, Mammalian / Q000378:metabolism*; D020869:Gene Expression Profiling / Q000379:methods*; D005796:Genes; D018392:Genomic Imprinting; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics*; D016366:Open Reading Frames; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D021101:Radiation Hybrid Mapping
D009687:Nuclear Proteins; D012333:RNA, Messenger; C441460:Rian protein, mouse