Immunoreactivity of VR1 on epidermal keratinocyte of human skin.
We demonstrated the immunoreactivity of the receptor proteins, VR1, ion channels associated with pain sensation, on the epidermis of the human skin. Immunohistochemistry using antiserum against VR1 derived peptide showed immunoreactivity on the keratinocytes cell membrane of the human epidermis and cultured keratinocytes. The blocking peptide of the antiserum reduced the immunoreactivity on the epidermis. RT-PCR assay of cultured human keratinocyte also showed expression of VR1 mRNA. These results suggest the existence of VR1-like protein in epidermal keratinocytes of human skin.
Denda
M
M
Shiseido Life Science Research Center, 2-12-1 Fukuura, Kanazawa-ku, Yokohama, 236-8643, Japan. mitsuhiro.denda@to.shiseido.co.jp
Fuziwara
S
S
Inoue
K
K
Denda
S
S
Akamatsu
H
H
Tomitaka
A
A
Matsunaga
K
K
D000328:Adult; D000368:Aged; D000918:Antibody Specificity; D002462:Cell Membrane / Q000737:chemistry / Q000648:ultrastructure; D000078404:Epidermal Cells; D004817:Epidermis / Q000737:chemistry; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D015603:Keratinocytes / Q000737:chemistry / Q000166:cytology*; D008297:Male; D008875:Middle Aged; D010446:Peptide Fragments / Q000276:immunology; D012333:RNA, Messenger / Q000032:analysis; D011955:Receptors, Drug / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D020133:Reverse Transcriptase Polymerase Chain Reaction
D010446:Peptide Fragments; D012333:RNA, Messenger; D011955:Receptors, Drug