[Study on the vaccine against dental caries of recombinant Streptococcus lactis and artificial active immunization. V. Extraction, purification and dot blot analysis of RNA in the recombinant S. lactis].
To investigate the transcription level of the pac gene in the recombinant S. lactis, the amount of PAc-specific mRNA was measured by RNA dot blot analysis with the biotin-labeled 1.5 kb PstI fragment of the pac gene. RNA were extracted with guanidinium thiocyanate followed by centrifugation in cesium chloride solutions. These results indicated the sequence of PAc-mRNA in S. lactis HL107 or HL45 was the same as that in S. mutans Ingbritt and showed similar density photograph. The PAc-specific mRNA plated an intermediary role of protein synthesis in recombinant S. lactis and made it complete the expression of cloned pac gene.
Ling
J
J
Sun Yat-sen University of Medical Sciences Affiliated Guangzhou Hospital of Stomatology, China.
Fan
M
M
D015152:Blotting, Northern; D003731:Dental Caries / Q000517:prevention & control*; D013294:Lactococcus lactis / Q000235:genetics / Q000276:immunology*; D012329:RNA, Bacterial / Q000302:isolation & purification*; D011995:Recombination, Genetic; D022541:Streptococcal Vaccines; D013295:Streptococcus mutans / Q000235:genetics
D012329:RNA, Bacterial; D022541:Streptococcal Vaccines