Comparison of a radioimmunoassay with a microparticle enzyme immunoassay of insulin for use with the minimal model method of determining whole-body insulin sensitivity.
The purpose of this study was to compare minimal model results of insulin sensitivity and glucose effectiveness using insulin levels measured by a conventional radioimmunoassay (RIA) versus an automated microparticle enzyme immunoassay (MEIA). Thirty obese subjects participated in an insulin-modified frequently sampled intravenous glucose tolerance test. The MEIA exhibited lower day-to-day variability than did the RIA. The MEIA yielded lower insulin values compared to the RIA probably because of the high cross-reactivity with proinsulin in the RIA. The MEIA yielded a good correlation with the RIA for both insulin sensitivity (r = 0.97, p = 0.0001) and for glucose effectiveness (r = 0.98, p = 0.0001). The MEIA did not significantly effect the results of the MinMod analysis and the low cross-reactivity with proinsulin makes MEIA preferable when insulin sensitivity (SI) is measured in patients with diabetes or obese individuals whose insulin:proinsulin ratio is altered.
Rood
J C
JC
Pennington Biomedical Research Center, Baton Rouge, Louisiana, USA. roodjc@mhs.pbrc.edu
Lovejoy
J C
JC
Tulley
R T
RT
D000328:Adult; D001331:Automation; D005215:Fasting; D005260:Female; D005951:Glucose Tolerance Test; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods; D007202:Indicators and Reagents; D007328:Insulin / Q000097:blood*; D007333:Insulin Resistance / Q000502:physiology*; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000097:blood / Q000503:physiopathology*; D011863:Radioimmunoassay / Q000379:methods; D011933:Reagent Kits, Diagnostic; D012044:Regression Analysis; D015203:Reproducibility of Results
D007202:Indicators and Reagents; D007328:Insulin; D011933:Reagent Kits, Diagnostic