title: Demonstration of Chlamydia pneumoniae in atherosclerotic arteries from various vascular regions.
abstract: Chlamydia pneumoniae (CP) has been reported to be a pathogenic agent in the mechanism leading to atherosclerosis. The majority of available data is focused mainly on coronary artery disease whereas the distribution of CP in different areas, associated with atherosclerotic disorders, has not been completely clarified. In this study we investigated the presence of CP in atheromasic plaques from five different vascular areas (basilary artery, coronary artery, thoracic aorta, abdominal aorta, renal arteries) using nested polymerase chain reaction (PCR) and immunohistochemical staining (IHC), in order to establish the putative association of CP with atherosclerotic disease. The same atheromasic plaques were also tested for the presence of Helicobacter pylori (HP) and cytomegalovirus (CMV), other putative agents of atherosclerosis, using a nested PCR technique. Our data indicate that the presence of CP can be demonstrated in 100% of patients tested, considering globally the five areas of analysis. On the other hand the presence of HP has been demonstrated in four out of 18 patients (22.2%), and CMV only in three out of 18 (16.6%). Our results strongly suggest an association between CP and atherosclerosis and highlight the need for the detection of CP in multiple vascular areas of the same patient.
authors.affiliation: Department of Microbiology, S. Bortolo Hospital, Via Rodolfi 37, 36100 Vicenza, Italy. microbvi@tin.it
authors.forename: M
authors.initials: M
authors.lastname: Rassu
authors.forename: S
authors.initials: S
authors.lastname: Cazzavillan
authors.forename: M
authors.initials: M
authors.lastname: Scagnelli
authors.forename: A
authors.initials: A
authors.lastname: Peron
authors.forename: P A
authors.initials: PA
authors.lastname: Bevilacqua
authors.forename: M
authors.initials: M
authors.lastname: Facco
authors.forename: G
authors.initials: G
authors.lastname: Bertoloni
authors.forename: F M
authors.initials: FM
authors.lastname: Lauro
authors.forename: R
authors.initials: R
authors.lastname: Zambello
authors.forename: E
authors.initials: E
authors.lastname: Bonoldi
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001011:Aorta / Q000382:microbiology; D001158:Arteries / Q000382:microbiology*; D001161:Arteriosclerosis / Q000382:microbiology*; D001488:Basilar Artery / Q000382:microbiology; D001921:Brain / Q000382:microbiology; D016993:Chlamydophila pneumoniae / Q000302:isolation & purification*; D003331:Coronary Vessels / Q000382:microbiology; D003587:Cytomegalovirus / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000032:analysis; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D016480:Helicobacter pylori / Q000302:isolation & purification; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012077:Renal Artery / Q000382:microbiology
chemical_list: D004269:DNA, Bacterial; D004279:DNA, Viral