Basal production of pentane in expired gas from healthy humans.
BACKGROUND
Pentane in exhaled gas is often used as an index of lipoperoxidation, but today, there is no standardization for its measurement. In this study, with our technical experience, we determined basal production of pentane in healthy subjects, and we evaluated variability of pentane flow 1 month later.


METHODS
18 subjects inhaled hydrocarbon-free air (HCFA) in order to realize a lung washout. Ambient air and three samples (at T0, T10, T30 min) of expired gas were concentrated using a "trap-and-purge" procedure. For the analysis of pentane, an Al(2)O(3)/KCl plot column contained in a gas chromatograph equipped with a flame ionization detector was used.


RESULTS
After 10 min of washout, mean (+/-SD) exhalation rate of pentane was 1+/-0.6 pmol min(-1) kg(-1). After 30 min of washout, mean (+/-SD) exhalation rate of pentane was 0.7+/-0.5 pmol min(-1) kg(-1). No significant difference in pentane flow was shown 1 month later for eight subjects who repeated the protocol.


CONCLUSIONS
With our results and data of the literature, exhalation rates of pentane from healthy adults appear to range between 0.3 and 2 pmol min(-1) kg(-1). The variability of pentane flow 1 month later seems not very important.
Loiseaux-Meunier
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MN
Laboratoire de Biochimie et Immunochimie, Hôpital Gabriel-Montpied, 30, place Henri-Dunant, 63000 Clermont-Ferrand, France. mnmeunier@chu-clermontferrand.fr
Bedu
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M
Gentou
C
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Pepin
D
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Coudert
J
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Caillaud
D
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D000328:Adult; D000368:Aged; D002849:Chromatography, Gas; D005260:Female; D005410:Flame Ionization; D006801:Humans; D015227:Lipid Peroxidation; D008297:Male; D008875:Middle Aged; D010420:Pentanes / Q000032:analysis / Q000378:metabolism*; D012016:Reference Values; D012119:Respiration
D010420:Pentanes; C033353:pentane