Assembly of pore proteins on gold electrodes.
Protein-protein interactions at the cell surface are important in the activity of bacterial toxins such as colicins. We have developed methods to study these events using tethered lipid bilayers, which can be probed by impedance spectroscopy and surface plasmon resonance. Recently we have attached the receptor proteins directly to gold electrodes and this offers new possibilities for measuring protein-protein interactions on solid supports.
Hong
Q
Q
School of Biochemistry and Genetics, The Medical School, Newcastle upon Tyne, NE2 4HH, UK.
Terrettaz
S
S
Ulrich
W P
WP
Vogel
H
H
Lakey
J H
JH
D001665:Binding Sites; D003087:Colicins / Q000737:chemistry / Q000378:metabolism; D004566:Electrodes; D006046:Gold; D008051:Lipid Bilayers; D018272:Porins / Q000096:biosynthesis* / Q000737:chemistry / Q000378:metabolism
D003087:Colicins; D008051:Lipid Bilayers; C109237:OmpF protein; D018272:Porins; D006046:Gold