[Augmented effect of Composite Radix Salviae Miltiorrhizae Injection on radiosensitivity of leukemia cells].
OBJECTIVE
To explore the effect of Composite Radix Salviae Miltiorrhizae Injection (CSMI) on radiosensitivity of leukemia cells.


METHODS
Semisolid agar culture and flow cytometry assay were performed for studying the change in radiosensitivity of HL60 cell line and fresh human leukemia cells after exposing to CSMI.


RESULTS
The D0(the inverse of the slope of the survival curve) and SF2(survival fraction at 2 Gy) of HL60 cell line were decreased from 1.53, 0.34 to 0.93, 0.12, respectively and apoptosis rates after radiation were raised significantly by CSMI. Furthermore, the concentration of CSMI and the time of mixed culture with CSMI before irradiation had positive relation to the effects mentioned above. Compared with control group, CSMI could increase the radiation-induced apoptosis of fresh leukemia cells (5.89 +/- 2.91% vs 12.05 +/- 3.06%).


CONCLUSIONS
CSMI could obviously enhance the radiosensitivity of leukemia cells.
Tan
H
H
Second Affiliated Hospital, Guangzhou Medical College, Guangzhou 510260.
Ying
H
H
Chu
J
J
D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D018922:HL-60 Cells / Q000187:drug effects; D006801:Humans; D007945:Leukemia, Lymphoid / Q000473:pathology*; D015470:Leukemia, Myeloid, Acute / Q000473:pathology*; D010936:Plant Extracts; D011838:Radiation-Sensitizing Agents / Q000494:pharmacology*; D027561:Salvia miltiorrhiza
D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; D011838:Radiation-Sensitizing Agents