[mRNA expression of metastasis-suppressor gene nm23 in carcinoma of buccal mucosa I. Northern blotting study].
In this study the authors detected both nm23-H1 and nm23-H2 mRNA levels in 52 tissues samples of patients with carcinoma of buccal mucosa (CBM) by Northern blotting method. The expression levels of both nm23-H1 and nm23-H2 mRNA in CBM were higher than those in normal buccal mucosa, leukoplakia, adjacent nontumorous mucosa, and lymph nodes with or without metastasis. The nm23-H1 mRNA expression levels in CBM with lymph nodes metastases were lower than those in CBM without metastasis, and were lower in metastatic lympho nodes (P < 0.05). Of the 11 cases with metastases, 9 cases (81.8%) showed low expression of nm23-H1 mRNA, of 19 cases without metastasis, 15 cases (78.9%) were high expression. No significance of nm23-H2 mRNA expression levels were found between CBM with and CBM without metastasis (P > 0.05). The expression of nm23-H1 and nm23-H2 mRNA showed no significant correlation with each clinical-pathological parameter examed. The result suggested that the expression of nm23-H1 mRNA significantly correlated inversely with lymph node metastasis in CBM, while the expression of nm23-H2 mRNA not. nm23 mRNA expression levels can provide a valuable predictor of lymph node metastases in CBM.
Lu
Y
Y
Department of Oral Pathology, College of Stomatology, West China University of Medical Sciences, China.
Li
C
C
Zhou
Z
Z
D000951:Antigens, Neoplasm / Q000096:biosynthesis; D014408:Biomarkers, Tumor; D015152:Blotting, Northern; D015870:Gene Expression; D016147:Genes, Tumor Suppressor; D006801:Humans; D007972:Leukoplakia, Oral / Q000235:genetics* / Q000378:metabolism; D008198:Lymph Nodes; D008207:Lymphatic Metastasis; D020559:Monomeric GTP-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics; D009061:Mouth Mucosa / Q000378:metabolism; D009062:Mouth Neoplasms / Q000235:genetics* / Q000378:metabolism; D054778:NM23 Nucleoside Diphosphate Kinases; D009701:Nucleoside-Diphosphate Kinase; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics
D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D054778:NM23 Nucleoside Diphosphate Kinases; D012333:RNA, Messenger; D014157:Transcription Factors; C517938:NME1 protein, human; D009701:Nucleoside-Diphosphate Kinase; D020559:Monomeric GTP-Binding Proteins