title: Binding of Plasmodium falciparum-infected erythrocytes to soluble platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31): frequent recognition by clinical isolates.
abstract: Platelet-endothelial cell adhesion molecule-1 or CD31 (PECAM-1/CD31) is a receptor recognized by Plasmodium falciparum-parasitized erythrocytes (pRBCs). Fluorescence-labeled soluble recombinant PECAM-1/CD31 (sPECAM-1/CD31) is shown to bind to the surface of P. falciparum-infected erythrocytes on up to 70% of the cells. Binding is blocked by the addition of the unlabeled receptor in a dose-dependent fashion, but not by unrelated receptor-proteins. A significant correlation was found between the binding of sPECAM-1/CD31 to pRBCs and the binding to transfected L cells expressing the receptor as seen with six different P. falciparum lines or clones. Panning of cultures on PECAM-1/CD31 transfected L cells was paralleled by an increase in the binding of sPECAM-1/CD31. The pRBCs of 54% of fresh patient-isolates bound sPECAM-1/CD31 with a mean rate of 12.9% (range = 1.1-44%). The data suggest that PECAM-1/CD31 is a common receptor recognized by wild isolates and that the soluble PECAM-1/CD31 suspension assay is a sensitive and reliable way to study PECAM-1/CD31 binding.
authors.affiliation: Microbiology and Tumor Biology Center, Karolinska Institutet and Swedish Institute for Infectious Disease Control, Stockholm.
authors.forename: A
authors.initials: A
authors.lastname: Heddini
authors.forename: Q
authors.initials: Q
authors.lastname: Chen
authors.forename: J
authors.initials: J
authors.lastname: Obiero
authors.forename: O
authors.initials: O
authors.lastname: Kai
authors.forename: V
authors.initials: V
authors.lastname: Fernandez
authors.forename: K
authors.initials: K
authors.lastname: Marsh
authors.forename: W A
authors.initials: WA
authors.lastname: Muller
authors.forename: M
authors.initials: M
authors.lastname: Wahlgren
mesh_terms: D000818:Animals; D016466:CHO Cells; D019556:COS Cells; D002448:Cell Adhesion; D002675:Child, Preschool; D006224:Cricetinae; D004912:Erythrocytes / Q000378:metabolism / Q000469:parasitology*; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D006834:Hydrazines / Q000737:chemistry; D007630:Kenya; D007739:L Cells; D016778:Malaria, Falciparum / Q000097:blood; D051379:Mice; D008856:Microscopy, Fluorescence; D010963:Plasmodium falciparum / Q000502:physiology*; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000097:blood* / Q000494:pharmacology; D011994:Recombinant Proteins / Q000378:metabolism / Q000494:pharmacology; D012995:Solubility; D014162:Transfection
chemical_list: C417561:Alexa 488 hydrazide; D005456:Fluorescent Dyes; D006834:Hydrazines; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D011994:Recombinant Proteins