[Evolution of isolation and identification techniques of waterborne viruses].
The paralytic potential of the poliovirus was recognized as early as the 14th century B.C. as illustrated in Egyptian art. But it is only after the four last decades that methods for their concentration from water and their identification were performed. Among several of them the adsorption-elution method was retained. Nevertheless two important barriers had to be ran-over. The first one was the concentration-elution steps on different materials which had to be improved. The second one was the typing method which had to move from particle by particle identification to entire viral population. Despite of these advances only a few cytopathogenic serotypes were found. The reverse transcriptase-polymerase chain reaction with its far more wide spectrum allows the fast and direct identification of viral nucleic acids (or their fragments) of almost all viruses, cytopathogenic or not. With this method elevated amounts of drinking water samples were found positive for several non cytopathogenic viruses. The sanitary significance of these results has still to be proved.
Vilaginès
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Centre de Recherche et de Contrôle des Eaux de la Ville de Paris, 144-156, avenue Paul Vaillant Couturier-75014 Paris.
D000327:Adsorption; D004279:DNA, Viral / Q000235:genetics; D005602:France; D006801:Humans; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014773:Virology / Q000379:methods* / Q000639:trends*; D014776:Virus Cultivation / Q000379:methods* / Q000639:trends*; D014780:Viruses / Q000235:genetics* / Q000302:isolation & purification*; D014871:Water Microbiology; D014881:Water Supply
D004279:DNA, Viral