[Trifluoperazine's action on fibroblast proliferation in cell cultures. Comparison of different doses].
OBJECTIVE
To evaluate the effect of different doses of trifluoperazine (TFP) on fibroblastic proliferation in cell cultures of sclera and conjunctiva.


METHODS
24 brown rabbits were operated on for non-protected sclerectomy and were divided into four groups. Group 1: Subconjunctival injection of 0.5 ml of balanced saline solution (BSS) 24 h before surgery. Group 2: Intraoperative TFP 10(-2)M for five minutes on the sclera in experimental filtrating surgery. Group 3: Subconjunctival injection of 0.5 ml of TFP 10(-3) M 24 h before surgery. Group 4: Subconjunctival injection of 0.5 ml of TFP 10-4M 24 h before surgery. Conjunctiva and sclera tissue samples were taken 1 hour after surgery in the treated area and at 90 degrees from it. All of the samples were cultured in DMEM with 20% fetal calf serum. The presence of atypical cells and fibroblastic growth was assessed at 3, 6, 9 and 12 days.


RESULTS
There were statistically significant differences in all the groups and in the times compared to the control group. In the treated conjunctiva, differences were found between the subconjunctiva 10(-3) M and the intraoperative 10(-2) M on the 3rd and 6th days, between the preoperative 10-2 and subconjunctiva 10(-3) M and 10(-4) after the 6th day. In conjunctiva at 90 degrees, there were significant differences between preoperative 10-2 M and subconjunctiva 10-3 after day 6, between the preoperative 10-2 M and subconjunctiva 10(-4) on days 9 and 12. There were no significant differences among the different treatments in the sclera samples, but there were differences between all them and the control group.


CONCLUSIONS
TFP at a dose of 10(-2) and 10(-4) TFP has an inhibitory effect on cellular proliferation in vitro in the rabbit sclera and conjunctiva. All the doses used produce a significant inhibition of cellular proliferation without producing significant disorders in the cellular morphology.
Izaguirre Roncal
L B
LB
Servicio de Oftalmología, Hospital Miguel Servet, Zaragoza, España.
Gonzalvo Ibáñez
F J
FJ
Pinilla Lozano
I
I
Pablo Júlvez
L
L
Abecia Martínez
E
E
Honrubia López
F M
FM
D000818:Animals; D002121:Calcium Channel Blockers / Q000008:administration & dosage*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects*; D011817:Rabbits; D014268:Trifluoperazine / Q000008:administration & dosage*
D002121:Calcium Channel Blockers; D014268:Trifluoperazine