Dentin barrier test with transfected bovine pulp-derived cells.
Growth kinetics of SV40 large T-antigen-transfected bovine pulp-derived cells on dentin were investigated. These cells were used in a dentin barrier test device, and the system was evaluated by testing a set of dental filling materials. Cells (120 cells/mm2) were seeded on dentin slices and incubated for up to 21 days. Cell proliferation was recorded using MTT assay. For cytotoxicity tests 3500 cells/mm2 were seeded on dentin discs, which were then incorporated into the dentin barrier test device. After 72 h preincubation test materials were applied. After a 24 h exposure with or without perfusion of the pulpal part of the test device, cell survival was evaluated using MTT assay. The cells revealed similar growth kinetics on dentin slices and on tissue culture plates. In cytotoxicity tests the cells were more sensitive toward the test materials than previously used three-dimensional cultures of human foreskin fibroblasts and as anticipated from clinical experience. Further improvement is expected by using three-dimensional cultures of pulp-derived cells.
Schmalz
G
G
Department of Operative Dentistry and Periodontology, University of Regensburg, Germany.
Schuster
U
U
Thonemann
B
B
Barth
M
M
Esterbauer
S
S
D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics; D001672:Biocompatible Materials / Q000633:toxicity*; D002417:Cattle; D018929:Cell Culture Techniques; D002455:Cell Division / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects; D016722:Cermet Cements / Q000633:toxicity; D002999:Clone Cells; D004396:Coloring Agents; D003738:Dental Cements / Q000633:toxicity*; D003782:Dental Pulp / Q000166:cytology / Q000187:drug effects*; D003804:Dentin / Q000187:drug effects*; D005347:Fibroblasts / Q000187:drug effects; D005899:Glass Ionomer Cements / Q000633:toxicity; D006801:Humans; D008855:Microscopy, Electron, Scanning; D019279:Resin Cements / Q000633:toxicity; D012826:Silicone Elastomers / Q000633:toxicity; D012867:Skin / Q000166:cytology / Q000187:drug effects; D013053:Spectrophotometry; D018709:Statistics, Nonparametric; D013778:Tetrazolium Salts; D013844:Thiazoles; D013997:Time Factors; D014162:Transfection; D015035:Zinc Oxide-Eugenol Cement / Q000633:toxicity; D015036:Zinc Phosphate Cement / Q000633:toxicity
D000952:Antigens, Polyomavirus Transforming; D001672:Biocompatible Materials; D016722:Cermet Cements; D004396:Coloring Agents; D003738:Dental Cements; D005899:Glass Ionomer Cements; D019279:Resin Cements; D012826:Silicone Elastomers; D013778:Tetrazolium Salts; D013844:Thiazoles; D015035:Zinc Oxide-Eugenol Cement; D015036:Zinc Phosphate Cement; C022616:thiazolyl blue