Simplified microneutralization test for serotyping adenovirus isolates.
A simplified microneutralization procedure is described that uses an empirically determined virus challenge dose, a single dilution of antiserum, and observation of cytopathic effect to determine the adenovirus serotype. The simplified test has faster turnaround time and was 96% concordant with a confirmatory test using serial dilutions of type-specific sera. This method will find utility in high-volume serotyping work.
Malasig
M D
MD
Department of Defense Center for Deployment Health Research, Naval Health Research Center, San Diego, California 92186, USA. malasig@nhrc.navy.mil
Goswami
P R
PR
Crawford-Miksza
L K
LK
Schnurr
D P
DP
Gray
G C
GC
D000258:Adenovirus Infections, Human / Q000821:virology; D000260:Adenoviruses, Human / Q000145:classification* / Q000302:isolation & purification; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D006801:Humans; D009500:Neutralization Tests; D010614:Pharynx / Q000821:virology; D012703:Serotyping