[Role of c-fos gene in the pulmonary surfactant synthesis of cultured alveolar type II cells induced by endothelin-1].
The effects of endothelin-1 (ET-1) on pulmonary surfactant (PS) synthesis of cultured alveolar type II cells (AT II) were observed. The role of c-fos gene in cellular signal transduction of ET-1 was studied by antisense technology. The results showed that: (1) ET-1 enhanced [3H] choline incorporation into AT II cells in a dose-dependent manner. (2) Protein kinase (PKC) activator PMA increased [3H] choline incorporation into AT II cells, while PKC inhibitor H7 inhibited the stimulating effect of ET-1. (3) Both ET-1 and PMA could increase the level of c-Fos protein, and H7 and c-fos antisense oligonucleotides (AS ODN) could inhibit the effects induced by ET-1 on Fos protein expression and [3H] choline incorporation. (4) The release of lactic dehydrogenase (LDH) was not different among control, ET-1, antisense oligonucleotides and sense oligonucleotides groups. The above results demonstrated that ET-1 can enhance PS synthesis of AT II cells and ET-1 stimulating the expression of c-fos gene mediated by PKC is a major signal transduction pathway of modulating PS synthesis.
Luo
Z Q
ZQ
Department of Physiology, Hunan Medical University, Changsha 410078.
Sun
X H
XH
Qin
X Q
XQ
D000818:Animals; D019332:Endothelin-1 / Q000494:pharmacology*; D005260:Female; D016762:Genes, fos; D008297:Male; D011493:Protein Kinase C / Q000494:pharmacology; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis; D011650:Pulmonary Alveoli / Q000166:cytology / Q000378:metabolism*; D011663:Pulmonary Surfactants / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction
D019332:Endothelin-1; D016760:Proto-Oncogene Proteins c-fos; D011663:Pulmonary Surfactants; D011493:Protein Kinase C