No evidence of HIV and SIV sequences in two separate lots of polio vaccines used in the first U.S. polio vaccine campaign.
We obtained sealed vials of two different polio vaccine lots, expiration date 1955, which were used in the first U.S. polio vaccine campaign. These early lots were pulled from the market because they contained live infectious poliovirus which caused polio in some of the vaccines. Theoretically, these vaccines could have contained other infectious retroviruses, including HIV. No viral sequences were detected using RT-PCR analyses with primers capable of amplifying chimpanzee SIV and HIV-1-related viruses nor with primers for macaque SIV, sooty mangabey SIV, and HIV-2-related viruses. Poliovirus sequences were readily amplified by RT-PCR, suggesting that the technique used would have detected SIV or HIV sequences, if present.
Rizzo
P
P
Cardinal Bernardin Cancer Center, Department of Pathology, Loyola University Chicago, Maywood, Illinois 60153, USA.
Matker
C
C
Powers
A
A
Setlak
P
P
Heeney
J L
JL
Carbone
M
M
D000818:Animals; D016665:Cercocebus atys; D004340:Drug Contamination; D006678:HIV / Q000302:isolation & purification*; D015497:HIV-1 / Q000302:isolation & purification; D015498:HIV-2 / Q000302:isolation & purification; D006801:Humans; D002679:Pan troglodytes; D023321:Poliovirus Vaccines / Q000737:chemistry*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015302:Simian Immunodeficiency Virus / Q000302:isolation & purification*
D023321:Poliovirus Vaccines