title: The influence of dissolved CO(2) concentration on the death kinetics of Saccharomyces cerevisiae.
abstract: OBJECTIVE
  The effects of temperature and concentration of dissolved CO(2) on the inactivation of Saccharomyces cerevisiae were investigated using a plug-flow system.
  
  
  RESULTS
  Several combinations of pressure (4, 6, 8, 10 mega-Pa (MPa)) and temperature (30, 34, 36, 38 degrees C) were used. The D-values obtained were 0.14 min at 8 MPa and 38 degrees C, and 0.15 min at 10 MPa and 36 degrees C. The log D-values were related linearly to the treatment temperature and to the dissolved CO(2) concentration. The thermal resistance constant (zCO(2)(T)) was 9.5 degrees C in the media, including significant levels of CO(2), and the CO(2) resistance constant was z(temp.)(gamma)=7.2 gamma.
  
  
  CONCLUSIONS
  This work has shown that inactivation followed first-order death kinetics, and the effects of temperature and CO(2) concentration were consistent through the critical temperature and pressure of CO(2). Therefore, it is feasible to estimate D-values at any temperature and any CO(2) concentration.
  
  
  CONCLUSIONS
  Non-thermal inactivation of micro-organisms in acidic beverages could be realized by the present technique.
authors.affiliation: Department of Bioscience and Biotechnology, Kyushu University, Fukuoka, Kyushu, Japan. mishimoda@agr.kyushu-u.ac.jp
authors.forename: M
authors.initials: M
authors.lastname: Shimoda
authors.forename: J
authors.initials: J
authors.lastname: Cocunubo-Castellanos
authors.forename: H
authors.initials: H
authors.lastname: Kago
authors.forename: M
authors.initials: M
authors.lastname: Miyake
authors.forename: Y
authors.initials: Y
authors.lastname: Osajima
authors.forename: I
authors.initials: I
authors.lastname: Hayakawa
mesh_terms: D002245:Carbon Dioxide / Q000378:metabolism / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D005519:Food Preservation / Q000379:methods*; D007700:Kinetics; D009997:Osmotic Pressure; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development*; D013696:Temperature
chemical_list: D002245:Carbon Dioxide