Correlation of genetic variability with safety of mumps vaccine Urabe AM9 strain.
The Urabe AM9 strain of mumps vaccine live is known for its genetic instability and some vaccines derived from this strain were withdrawn from the market due to an excessive number of vaccine-associated parotitis and meningitis cases. To identify the molecular basis of this instability, we determined complete nucleotide sequences of several stocks of the Urabe strain used for vaccine production by different manufacturers and of two clinical isolates from cases of vaccine-associated meningitis. In contrast to previously published studies relating the Lys335 --> Glu mutation in the viral HN gene with neurovirulence of mumps virus, we could not confirm any association of this mutation with the safety of mumps vaccine. Each of the three vaccine stocks studied had its own characteristic profile of mutations that was identified by cDNA sequencing and quantitated by mutant analysis by PCR and restriction enzyme cleavage. Determination of the mutational profile of mumps vaccine lots could allow vaccine manufacturers to characterize seed viruses and monitor the consistency of vaccine production to prevent emergence of virulent revertants.
Amexis
G
G
Center for Biologics Evaluation and Research, Food and Drug Administration, Rockville, Maryland 20852, USA.
Fineschi
N
N
Chumakov
K
K
D019943:Amino Acid Substitution; D001483:Base Sequence; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D014644:Genetic Variation; D005973:Glutamine / Q000235:genetics; D015707:HN Protein / Q000235:genetics; D008239:Lysine / Q000235:genetics; D008581:Meningitis / Q000821:virology; D008969:Molecular Sequence Data; D009108:Mumps Vaccine / Q000235:genetics*; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping
D015707:HN Protein; D009108:Mumps Vaccine; D005973:Glutamine; D008239:Lysine