Patterns of gene expression during Drosophila mesoderm development.
The transcription factor Twist initiates Drosophila mesoderm development, resulting in the formation of heart, somatic muscle, and other cell types. Using a Drosophila embryo sorter, we isolated enough homozygous twist mutant embryos to perform DNA microarray experiments. Transcription profiles of twist loss-of-function embryos, embryos with ubiquitous twist expression, and wild-type embryos were compared at different developmental stages. The results implicate hundreds of genes, many with vertebrate homologs, in stage-specific processes in mesoderm development. One such gene, gleeful, related to the vertebrate Gli genes, is essential for somatic muscle development and sufficient to cause neural cells to express a muscle marker.
Furlong
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EE
Department of Developmental Biology, Howard Hughes Medical Institute, Beckman Center B300, Stanford University School of Medicine, Stanford, CA 94305-5329, USA.
Andersen
E C
EC
Null
B
B
White
K P
KP
Scott
M P
MP
D000818:Animals; D004330:Drosophila / Q000196:embryology* / Q000235:genetics; D029721:Drosophila Proteins; D004475:Ectoderm / Q000166:cytology; D004625:Embryo, Nonmammalian / Q000378:metabolism; D047108:Embryonic Development; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D017344:Genes, Insect; D017403:In Situ Hybridization; D019476:Insect Proteins / Q000235:genetics; D008562:Membrane Glycoproteins / Q000235:genetics; D008648:Mesoderm / Q000166:cytology / Q000378:metabolism*; D009154:Mutation; D009687:Nuclear Proteins / Q000235:genetics* / Q000502:physiology; D020411:Oligonucleotide Array Sequence Analysis; D011956:Receptors, Cell Surface; D051193:Toll-Like Receptors; D014157:Transcription Factors; D014158:Transcription, Genetic; D051879:Twist-Related Protein 1
D029721:Drosophila Proteins; D019476:Insect Proteins; D008562:Membrane Glycoproteins; D009687:Nuclear Proteins; D011956:Receptors, Cell Surface; C068721:Tl protein, Drosophila; D051193:Toll-Like Receptors; D014157:Transcription Factors; C497753:Twi protein, Drosophila; D051879:Twist-Related Protein 1