title: Cryopreservation of epididymal spermatozoa collected by needle biopsy from cynomolgus monkeys (Macaca fascicularis).
abstract: We have examined the motility, morphology, and cryopreservation of epididymal spermatozoa collected by needle biopsy from cynomolgus monkeys (Macaca fascicularis). At collection, epididymal sperm (23 x 10(6) +/- 4 x 10(6) sperm/sample; 611 x 10(6) +/- 116 x 10(6) sperm/ ml; n = 18) were alive (79 +/- 2%), motile (67 +/- 2%), and exhibited intact membranes (65 +/- 2%). Sperm maintained at room temperature in handling medium exhibited decreased motility over time, but head-to-head agglutination was limited. Tris egg-yolk extender containing 6% glycerol and dimethylsulfoxide (DMSO) did not significantly affect functional morphology, whereas extender containing propanediol significantly reduced motility, survival, and membrane integrity. Cryostorage reduced all measures of functional morphology independent of cryoprotectant. Post-thaw motility was superior for glycerol and DMSO compared to propanediol. Variation in glycerol concentration (4, 6, and 8%) produced equivocal effects on sperm functional morphology post-thaw. Needle biopsy may be a useful technique for laboratory and field-based collection of spermatozoa from nonhuman primates.
authors.affiliation: Pusat Studi Satwa Primata, Institut Pertanian Bogor, Indonesia.
authors.forename: A H
authors.initials: AH
authors.lastname: Feradis
authors.forename: D
authors.initials: D
authors.lastname: Pawitri
authors.forename: I K
authors.initials: IK
authors.lastname: Suatha
authors.forename: M R
authors.initials: MR
authors.lastname: Amin
authors.forename: T L
authors.initials: TL
authors.lastname: Yusuf
authors.forename: D
authors.initials: D
authors.lastname: Sajuthi
authors.forename: I N
authors.initials: IN
authors.lastname: Budiarsa
authors.forename: E S
authors.initials: ES
authors.lastname: Hayes
mesh_terms: D000818:Animals; D001707:Biopsy, Needle; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary*; D008252:Macaca fascicularis; D008297:Male; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D013081:Sperm Motility; D013094:Spermatozoa / Q000502:physiology*; D013696:Temperature; D013737:Testis