An in vitro model of neural trauma: device characterization and calcium response to mechanical stretch.
An in vitro model for neural trauma was characterized and validated. The model is based on a novel device that is capable of applying high strain rate, homogeneous, and equibiaxial deformation to neural cells in culture. The deformation waveform is fully arbitrary and controlled via closed-loop feedback. Intracellular calcium ([Ca2+]i) alterations were recorded in real time throughout the imposed strain with an epifluorescent microscopy system. Peak change in [Ca2+]i recovery of [Ca2+]i and percent responding NG108-15 cells were shown to be dependent on strain rate (1(-1) to 10(-1)) and magnitude (0.1 to 0.3 Green's Strain). These measures were also shown to depend significantly on the interaction between strain rate and magnitude. This model for neural trauma is a robust system that can be used to investigate the cellular tolerance and response to traumatic brain injury.
Geddes
D M
DM
School of Biomedical Engineering, Georgia Institute of Technology, Atlanta 30332-0405, USA.
Cargill
R S
RS
D000704:Analysis of Variance; D001696:Biomechanical Phenomena; D002118:Calcium / Q000378:metabolism / Q000502:physiology*; D006706:Homeostasis; D008954:Models, Biological; D020196:Trauma, Nervous System / Q000378:metabolism / Q000503:physiopathology*
D002118:Calcium