Cell growth and by-product formation in a pyruvate kinase mutant of E. coli.
In this paper, we report on the analysis of acid formation in an E. coli pyk mutant. The results demonstrate that acid formation is insignificant for both the wild-type and the mutant at low glucose concentrations. However, at relatively high glucose concentrations, acid formation remains very low for the mutant but is significant for the wild-type. This substantial reduction in acids is accompanied by an increase in CO(2) production. Moreover, unlike the B. subtilis pyk mutant, the E. coli pyk mutant did not show a substantial increase in the PEP pool.
Zhu
T
T
Department of Chemical Engineering, University of Pittsburgh, Pittsburgh, Pennsylvania 15219, USA.
Phalakornkule
C
C
Koepsel
R R
RR
Domach
M M
MM
Ataai
M M
MM
D000143:Acids / Q000378:metabolism; D001412:Bacillus subtilis / Q000235:genetics / Q000378:metabolism; D002245:Carbon Dioxide / Q000378:metabolism; D002455:Cell Division / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D009154:Mutation; D010728:Phosphoenolpyruvate / Q000378:metabolism; D011770:Pyruvate Kinase / Q000235:genetics* / Q000378:metabolism
D000143:Acids; D002245:Carbon Dioxide; D010728:Phosphoenolpyruvate; D011770:Pyruvate Kinase; D005947:Glucose