title: Thermal inactivation kinetics of bovine cathepsin D.
abstract: Cathepsin D, the principal indigenous acid proteinase in bovine milk, is a lysosomal proteinase, which exists in milk in four forms, including the inactive zymogen procathepsin D. The thermal inactivation kinetics of bovine cathepsin D, isolated from spleen and milk, were studied under isothermal conditions, using a specific HPLC assay to determine residual activity. Inactivation of the blood enzyme preparation followed first order kinetics, with z-values in phosphate buffer (pH 6.7) and skimmed milk of 6.5 and 7.6 degrees C, respectively, the enzyme being far more stable in the latter environment. Inactivation kinetics of the enzyme purified from milk were more complex, and could be best approximated by a double exponential model. Again, stability was higher in milk than in buffer. The double exponential model may indicate differing heat stabilities of isoforms of the enzyme, or stabilization of the enzyme by some milk constituent. It is clear that the enzyme can survive, at least partially, processes such as heating at 55 degrees C for 30 min during manufacture of high-cook cheese varieties (45% survival), and HTST pasteurization (8% survival), and thus may contribute to proteolysis in a range of dairy products.
authors.affiliation: Department of Food Science, Food Technology and Nutrition, University College, Cork, Ireland.
authors.forename: M G
authors.initials: MG
authors.lastname: Hayes
authors.forename: M J
authors.initials: MJ
authors.lastname: Hurley
authors.forename: L B
authors.initials: LB
authors.lastname: Larsen
authors.forename: C W
authors.initials: CW
authors.lastname: Heegaard
authors.forename: A A
authors.initials: AA
authors.lastname: Magboul
authors.forename: J C
authors.initials: JC
authors.lastname: Oliveira
authors.forename: P L
authors.initials: PL
authors.lastname: McSweeney
authors.forename: A L
authors.initials: AL
authors.lastname: Kelly
mesh_terms: D000818:Animals; D002402:Cathepsin D / Q000037:antagonists & inhibitors* / Q000378:metabolism; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods / Q000662:veterinary; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008892:Milk / Q000201:enzymology*; D011489:Protein Denaturation; D020033:Protein Isoforms; D013154:Spleen / Q000201:enzymology*; D013696:Temperature
chemical_list: D020033:Protein Isoforms; D002402:Cathepsin D