title: Olive oil decreases both oxidative stress and the production of arachidonic acid metabolites by the prostaglandin G/H synthase pathway in rat macrophages.
abstract: Fish oil has a preventive role in cardiovascular and inflammatory diseases, but little is known about the effect of olive oil, which is widely consumed in Mediterranean regions. We examined the influence of dietary olive oil, corn oil and fish oil-rich diets on the production of superoxide anion (O2-) and nitric oxide (.NO) by resident macrophages stimulated by phorbol 12-myristate 13-acetate (PMA) and their effect on arachidonic acid release, prostaglandin G/H synthase-2 (PGHS-2) expression and the subsequent prostaglandin E(2) production. Resident peritoneal macrophages stimulated by PMA from rats fed with olive oil or corn oil had the same level of O2- production, but these levels were increased by the fish oil diet. Olive oil and the fish oil diets increased .NO and decreased arachidonic acid mobilization and the production of prostaglandin E(2). PGHS-2 expression, however, was not affected by diet. We conclude that although olive oil and fish oil reduce arachidonic acid mobilization and subsequent metabolism through the PGHS-2 pathway in PMA-stimulated macrophages, only olive oil offers an additional beneficial effect by increasing .NO/O2- production.
authors.affiliation: Department of Physiology, Faculty of Pharmacy, University of Barcelona, 08028 Barcelona, Spain.
authors.forename: J J
authors.initials: JJ
authors.lastname: Moreno
authors.forename: T
authors.initials: T
authors.lastname: Carbonell
authors.forename: T
authors.initials: T
authors.lastname: Sánchez
authors.forename: S
authors.initials: S
authors.lastname: Miret
authors.forename: M T
authors.initials: MT
authors.lastname: Mitjavila
mesh_terms: D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism*; D003314:Corn Oil / Q000008:administration & dosage / Q000494:pharmacology; D015232:Dinoprostone / Q000096:biosynthesis*; D005395:Fish Oils / Q000008:administration & dosage / Q000494:pharmacology; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D009569:Nitric Oxide / Q000096:biosynthesis; D000069463:Olive Oil; D018384:Oxidative Stress / Q000187:drug effects*; D010938:Plant Oils / Q000008:administration & dosage / Q000494:pharmacology*; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013481:Superoxides / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D005395:Fish Oils; D000069463:Olive Oil; D010938:Plant Oils; D013481:Superoxides; D016718:Arachidonic Acid; D009569:Nitric Oxide; D003314:Corn Oil; D011451:Prostaglandin-Endoperoxide Synthases; D015232:Dinoprostone; D013755:Tetradecanoylphorbol Acetate