Expression of Arabidopsis GAI in transgenic rice represses multiple gibberellin responses.
Bioactive gibberellins (GAs) are essential endogenous regulators of plant growth. GA signaling is mediated via GAI, a nuclear member of the GRAS family of plant transcription factors. Previous experiments have suggested that GAI is a GA-derepressible repressor of plant growth. Here we test this hypothesis by examining the effects of the expression of Arabidopsis GAI in transgenic Basmati rice. High-level expression of GAI caused dwarfism and reduced GA responses, and the strength of this effect was correlated with the level of transgene expression. In particular, the expression of GAI abolished the GA-mediated induction of rice aleurone alpha-amylase activity, thus implicating GAI orthologs in the well-characterized cereal aleurone GA response. The GA derepressible repressor model predicts that high-level expression of GAI should confer dwarfism, and these observations are consistent with this prediction.
Fu
X
X
John Innes Centre, Norwich Research Park, Colney Lane, Norwich NR4 7UH, United Kingdom.
Sudhakar
D
D
Peng
J
J
Richards
D E
DE
Christou
P
P
Harberd
N P
NP
D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins; D001483:Base Sequence; D003001:Cloning, Molecular; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D005875:Gibberellins / Q000378:metabolism*; D006899:Mixed Function Oxygenases / Q000235:genetics; D008969:Molecular Sequence Data; D012275:Oryza / Q000201:enzymology / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D010937:Plant Growth Regulators / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000201:enzymology / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D012333:RNA, Messenger / Q000235:genetics; D015854:Up-Regulation; D000516:alpha-Amylases / Q000037:antagonists & inhibitors / Q000378:metabolism
D029681:Arabidopsis Proteins; C109555:GAI protein, Arabidopsis; D005875:Gibberellins; D010937:Plant Growth Regulators; D010940:Plant Proteins; D012333:RNA, Messenger; D006899:Mixed Function Oxygenases; C089210:gibberellin, 2-oxoglutarate-oxygen oxidoreductase (20-hydroxylating, oxidizing); D000516:alpha-Amylases