title: Identification of the first trypanosome H/ACA RNA that guides pseudouridine formation on rRNA.
abstract: In trypanosomes small nucleolar RNA (snoRNA) genes are clustered, and the clusters encode for either single or multiple RNAs. We previously reported on a genomic locus in Leptomonas collosoma that encodes for multiple C/D snoRNAs whose expression is regulated at the processing level (Xu, Y., Liu, L., Lopez-Estraño, C., and Michaeli, S. (2001) J. Biol. Chem. 276, 14289-14298). In this study we have characterized, in the same genomic locus, the first trypanosome H/ACA RNA, which we termed h1. Having a length of 69 nucleotides, h1 has the potential to guide pseudouridylation on 28 S rRNA. The h1 is processed from a long polycistronic transcript that carries both the C/D and h1 snoRNAs. The h1/rRNA duplex obeys the rules for guiding pseudouridylation. Mapping of the pseudouridine site indicated that the predicted U is indeed modified. However, in contrast to all H/ACA RNAs, h1 consists of a single hairpin structure and is the shortest H/ACA RNA described so far.
authors.affiliation: Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan 52900, Israel.
authors.forename: X H
authors.initials: XH
authors.lastname: Liang
authors.forename: L
authors.initials: L
authors.lastname: Liu
authors.forename: S
authors.initials: S
authors.lastname: Michaeli
mesh_terms: D000818:Animals; D001483:Base Sequence; D017125:Genes, Protozoan; D008969:Molecular Sequence Data; D005810:Multigene Family; D009690:Nucleic Acid Conformation; D011560:Pseudouridine / Q000096:biosynthesis*; D017393:RNA Editing; D017394:RNA, Guide, Kinetoplastida / Q000235:genetics / Q000378:metabolism*; D016053:RNA, Protozoan / Q000235:genetics / Q000378:metabolism*; D012335:RNA, Ribosomal / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic; D014351:Trypanosomatina / Q000235:genetics*
chemical_list: D016053:RNA, Protozoan; D012335:RNA, Ribosomal; D011560:Pseudouridine; D017394:RNA, Guide, Kinetoplastida