title: Exon/intron organization and transcription units of the human acyl-CoA synthetase 4 gene.
abstract: Acyl-CoA synthetase 4 (ACS4) is an arachidonate-preferring isozyme of ACS family predominantly expressed in steroidogenic tissues. Isolation and characterization of genomic clones encoding human ACS4 revealed that the genomic organization of the gene. The human ACS4 gene spans approximately 90 kb and consists of 16 exons. Sequence inspection of the 5'-flanking region revealed potential DNA elements including GATAs, p300, AP-4, SRY, CREB and MyoD. A minimal promoter region required for the expression of ACS4 in HeLa S3 cells was determined. The human ACS4 gene was mapped between the STS markers, WI-17685 and CHLC.GATA81B07 on Xq22-23 region.
authors.affiliation: Pharmacology and Molecular Biology Research Laboratories, Sankyo Company, Ltd., Tokyo 140-8710, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Minekura
authors.forename: M J
authors.initials: MJ
authors.lastname: Kang
authors.forename: Y
authors.initials: Y
authors.lastname: Inagaki
authors.forename: Y Y
authors.initials: YY
authors.lastname: Cho
authors.forename: H
authors.initials: H
authors.lastname: Suzuki
authors.forename: T
authors.initials: T
authors.lastname: Fujino
authors.forename: T T
authors.initials: TT
authors.lastname: Yamamoto
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003066:Coenzyme A Ligases / Q000235:genetics*; D004247:DNA; D005091:Exons; D006367:HeLa Cells; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic
chemical_list: D004247:DNA; D003066:Coenzyme A Ligases