[Protection of spinal motorneurons of section sciatic nerve by transplantation of NT-4 genetically expression cells into the side of section].
The NT-4 genetically engineered cells were made by infecting L-6TG cell (a rat myoblast cell line) in vitro with a retroviral vector pN2A containing the rat NT-4 cDNA. The bioactivities were determined by bioassay of PC12 cell survival rate. The rat with left sciatic nerve transaction was used as a model for treatment by implanting NT-4 genetically modified cell. The condition of motorneurons was assessed by Nissl stain and ChE stain. The results showed that: (1) the percentage of surviving Nissl-stained neurons on the lessened side of NT-4 (+) grafts significantly increased as compared to that with NT-4 (-) grafts 2-3 weeks and 3 months after sciatic nerve transaction, and (2) the grafted cells produced significant increase in the positive ChE stained area after sciatic nerve transaction in 1-3 weeks. Our observations indicate that adult motor neurons are still able to respond to neurotrophic factors and they may require the factors for survival.
Shi
J
J
Department of Neurobiology, Second Military Medical University, Shanghai 200433.
Qu
S
S
Lu
C L
CL
He
X L
XL
Wang
C H
CH
D000818:Animals; D002460:Cell Line; D017690:Cell Transplantation; D004622:Embryo, Mammalian; D005818:Genetic Engineering; D009046:Motor Neurons / Q000502:physiology*; D018485:Muscle Fibers, Skeletal / Q000166:cytology*; D009414:Nerve Growth Factors / Q000096:biosynthesis / Q000502:physiology*; D018696:Neuroprotective Agents; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000293:injuries; D013116:Spinal Cord / Q000503:physiopathology
D009414:Nerve Growth Factors; D018696:Neuroprotective Agents; C073922:neurotrophin 4