title: Evaluation of an anesthetic regimen for retroorbital blood collection from mice.
abstract: Many Institutional Animal Care and Use Committees require anesthetization of mice for retroorbital blood collection procedures. Optimal anesthetic regimens should provide chemical restraint and adequate analgesia for a sufficient but otherwise minimal duration. This study was conducted to evaluate the effectiveness, safety, and practical utility of the general anesthetic combination of ketamine and medetomidine with and without the topical anesthetic proparacaine. In addition, we sought to evaluate the reversal of general anesthesia with atipamezole. The findings suggest that the combination of ketamine, medetomidine, proparacaine, and atipamezole is a safe and humane anesthetic regimen for retroorbital collection of blood samples from mice.
authors.affiliation: Department of Infectious Diseases, St. Jude Children's Research Hospital, 332 N. Lauderdale, Memphis, TN 38105, USA.
authors.forename: R
authors.initials: R
authors.lastname: Taylor
authors.forename: K E
authors.initials: KE
authors.lastname: Hayes
authors.forename: L A
authors.initials: LA
authors.lastname: Toth
mesh_terms: D000317:Adrenergic alpha-Antagonists / Q000008:administration & dosage; D000700:Analgesics / Q000008:administration & dosage; D000758:Anesthesia / Q000379:methods; D000779:Anesthetics, Local / Q000008:administration & dosage*; D000827:Animal Welfare; D000818:Animals; D001800:Blood Specimen Collection / Q000379:methods / Q000662:veterinary*; D004347:Drug Interactions; D004359:Drug Therapy, Combination; D005260:Female; D007093:Imidazoles / Q000008:administration & dosage; D007649:Ketamine; D008297:Male; D020926:Medetomidine / Q000008:administration & dosage; D051379:Mice; D008815:Mice, Inbred Strains; D009915:Orbit / Q000098:blood supply*; D011430:Propoxycaine / Q000008:administration & dosage; D051381:Rats
chemical_list: D000317:Adrenergic alpha-Antagonists; D000700:Analgesics; D000779:Anesthetics, Local; D007093:Imidazoles; C050701:atipamezole; D007649:Ketamine; C005717:proxymetacaine; D011430:Propoxycaine; D020926:Medetomidine