Impairment in the expression and activity of Fyn during differentiation of naive CD4+ T cells into the Th2 subset.
We previously showed that the amounts of Fyn protein in Th2 clones were approximately one-third to one-fifth of those in Th1 clones. In this study we examined the role of Fyn in the polarization of naive CD4+ T cells toward the Th2 subset using fyn(-/-) mice. The fyn(-/-) naive CD4+ T cells efficiently produced Th2 cytokines and polarized toward the Th2 subset even in the absence of IL-4 and IL-13. The expression of Fyn in wild-type CD4+ T cells decreased at a transcription level concomitant with polarization toward the Th2 subset. These results suggest that Fyn plays a role in the down-regulation of the differentiation of naive CD4+ T cells into the Th2 subset.
Tamura
T
T
Department of Allergology, Institute of Medical Science, University of Tokyo, Tokyo, Japan. toshikit@ims.u-tokyo.ac.jp
Igarashi
O
O
Hino
A
A
Yamane
H
H
Aizawa
S
S
Kato
T
T
Nariuchi
H
H
D000818:Animals; D018106:CD28 Antigens / Q000276:immunology / Q000502:physiology; D017252:CD3 Complex / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology* / Q000201:enzymology / Q000276:immunology; D002454:Cell Differentiation / Q000235:genetics / Q000276:immunology; D016207:Cytokines / Q000096:biosynthesis; D004789:Enzyme Activation / Q000235:genetics / Q000276:immunology; D007106:Immune Sera / Q000494:pharmacology; D007399:Interphase / Q000235:genetics / Q000276:immunology*; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck) / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis* / Q000172:deficiency / Q000235:genetics / Q000502:physiology; D051076:Proto-Oncogene Proteins c-fyn; D015398:Signal Transduction / Q000235:genetics / Q000276:immunology; D018417:Th1 Cells / Q000166:cytology / Q000201:enzymology / Q000276:immunology; D018418:Th2 Cells / Q000166:cytology* / Q000201:enzymology / Q000276:immunology
D018106:CD28 Antigens; D017252:CD3 Complex; D016207:Cytokines; D007106:Immune Sera; D011518:Proto-Oncogene Proteins; D011505:Protein-Tyrosine Kinases; C494960:Fyn protein, mouse; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck); D051076:Proto-Oncogene Proteins c-fyn