1-Methyl-3-isobutylxanthine delays apoptosis induced by deprivation of growth factors in vascular endothelial cells.
OBJECTIVE
To investigate the effects of 1-methyl-3-isobutylxanthine (MIBX) on apoptosis induced by deprivation of acidic fibroblast growth factor (aFGF) and serum in vascular endothelial cells (VEC).


METHODS
Nuclear fragmentation was observed by fluorescence microscopy. Viability was determined by counting the cells that attached to dishes after treatments. DNA fragmentation was measured by agarose gel electrophoresis.


RESULTS
The cells deprived of aFGF and serum were treated with MIBX 25-200 mumol/L for 3, 6, 9, and 12 h, respectively. Morphological changes including the formation of apoptotic bodies and DNA fragmentation of these cells were significantly suppressed by IBMX 50-200 mumol/L at 3 h and 6 h. But after 12-h treatment, no difference was observed between the treated and untreated cells.


CONCLUSIONS
MIBX delays apoptosis of vascular endothelial cells induced by deprivation of aFGF and serum.
Miao
J Y
JY
Institute of Developmental Biology, School of Life Science, Shandong University, Jinan 250100, China.
Araki
S
S
Zhang
S L
SL
Hayashi
H
H
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002478:Cells, Cultured; D053938:DNA Fragmentation / Q000187:drug effects; D004730:Endothelium, Vascular / Q000166:cytology*; D016220:Fibroblast Growth Factor 1 / Q000494:pharmacology; D006801:Humans; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology*; D014471:Umbilical Veins / Q000166:cytology
D010726:Phosphodiesterase Inhibitors; D016220:Fibroblast Growth Factor 1; D015056:1-Methyl-3-isobutylxanthine