title: Feeding-related immune responsive brain stem neurons: association with CGRP.
abstract: Using dual-labeling in situ hybridization histochemistry, the neurotransmitter expression of immune-responsive neurons in the pontine parabrachial nucleus, a major relay for interoceptive information, was investigated. Intravenous injection of bacterial wall lipopolysaccharide resulted in dense c-fos mRNA expression in the external lateral parabrachial nucleus, and a majority of the c-fos expressing cells also expressed calcitonin gene-related peptide (CGRP) mRNA. In contrast CGRP-positive cells in the adjoining external medial subnucleus were c-fos negative. Taken together with previous hodological and behavioral studies, these data suggest that CGRPergic parabrachial neurons may mediate lipopolysaccharide-induced anorexia by means of their projection to central nucleus of the amygdala.
authors.affiliation: Division of Cell Biology, Department of Biomedicine and Surgery, Faculty of Health Sciences, University of Linköping, S-581 85 Linköping, Sweden.
authors.forename: J
authors.initials: J
authors.lastname: Paues
authors.forename: D
authors.initials: D
authors.lastname: Engblom
authors.forename: L
authors.initials: L
authors.lastname: Mackerlova
authors.forename: A
authors.initials: A
authors.lastname: Ericsson-Dahlstrand
authors.forename: A
authors.initials: A
authors.lastname: Blomqvist
mesh_terms: D000210:Acute-Phase Reaction; D000679:Amygdala / Q000502:physiology; D000818:Animals; D000855:Anorexia / Q000276:immunology / Q000503:physiopathology*; D015740:Calcitonin Gene-Related Peptide / Q000235:genetics*; D005247:Feeding Behavior / Q000502:physiology; D015870:Gene Expression / Q000276:immunology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D009474:Neurons / Q000276:immunology*; D011149:Pons / Q000166:cytology / Q000276:immunology*; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D008070:Lipopolysaccharides; D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger; D015740:Calcitonin Gene-Related Peptide