Isolation of a cDNA encoding an IgG immunoreactive antigen (zinc finger protein) of Strongyloides stercoralis.
A full length cDNA encoding an IgG immunoreactive antigen of Strongyloides stercoralis is described. A clone containing 1,328 bp insert was selected following screening of S. stercoralis cDNA library with an IgG fraction obtained from a pool of 78 S. stercoralis positive human sera samples. The nucleotide sequence of the 1,328 bp insert was found to be 70.5% A/T, reflecting a characteristic A/T codon bias of S. stercoralis. The nucleotide sequence of this insert identified a cDNA coding for a zinc finger protein. The conceptually translated amino acid sequence of the open reading frame for the IgG immunoreactive antigen of S. stercoralis encodes a 211 amino acid residue protein with an apparent molecular weight of 22.8 kDa and a predicted isoelectric point of 8.71. The diagnostic potential of this IgG immunoreactive antigen of S. stercoralis is also discussed.
Siddiqui
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Department of Internal Medicine, Texas Tech University Health Sciences Center, Amarillo VA Heath Care System, Amarillo, Texas 79106, USA. siddiqui@cortex.ama.ttuhsc.edu
Stanley
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Berk
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D000595:Amino Acid Sequence; D000818:Animals; D000947:Antigens, Helminth / Q000235:genetics* / Q000276:immunology*; D003062:Codon; D018076:DNA, Complementary / Q000302:isolation & purification*; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology*; D007526:Isoelectric Point; D008970:Molecular Weight; D016366:Open Reading Frames; D017171:Strongyloides stercoralis / Q000276:immunology*; D013322:Strongyloidiasis / Q000235:genetics / Q000276:immunology; D016335:Zinc Fingers / Q000276:immunology*
D000947:Antigens, Helminth; D003062:Codon; D018076:DNA, Complementary; D007074:Immunoglobulin G