title: Aging-dependent changes in the effect of daily melatonin supplementation on rat metabolic and behavioral responses.
abstract: Pineal melatonin secretion has been reported to commonly decrease with aging, whereas intra-abdominal adiposity, plasma insulin and plasma leptin levels tend to increase. We recently demonstrated that daily melatonin administration starting at middle age suppressed male rat intra-abdominal fat, plasma leptin and plasma insulin to youthful levels, suggesting that aging-related changes in pineal melatonin secretion and in energy regulation may be functionally related. Accordingly, we have now investigated the effects of daily melatonin treatment on energy regulation in young versus middle-aged male Sprague Dawley rats. Addition of melatonin to the drinking water (0.2 microg/mL) produced nocturnal and diurnal plasma melatonin concentrations in middle-aged rats (12 months) equivalent to those of young adult (5 months) rats. Administration of this melatonin dosage every day for 10 wk starting at 10 months of age suppressed (P < 0.01) relative intra-abdominal fat, non-fasted plasma insulin and plasma leptin by 27, 39, and 51%, respectively (vs. vehicle-treated controls). In contrast, administration of melatonin for 10 wk starting at 3 months of age did not significantly alter (P> 0.10) any of these parameters. The melatonin administration stimulated (102%, P < 0.001) behavioral responsiveness of the middle-aged rats in a test of response to novelty, restoring youthful levels, but did not significantly alter behavioral responsiveness of the young rats. These results suggest that suppression of intra-abdominal adiposity and plasma leptin and insulin levels and stimulation of behavioral responsiveness in response to daily exogenous melatonin begins at middle age, coincident with and likely dependent upon the aging-associated decline in endogenous pineal melatonin secretion. These results further suggest that appropriate melatonin supplementation may potentially provide therapy or prophylaxis not only for the insulin resistance, increased intra-abdominal fat and resulting pathologies that occur with aging, but also for some aging-associated behavioral changes.
authors.affiliation: VA Puget Sound Health Care System, Department of Psychiatry and Behavioral Sciences, University of Washington, Seattle 98195, USA. drasmuss@u.washington.edu
authors.forename: D D
authors.initials: DD
authors.lastname: Rasmussen
authors.forename: D R
authors.initials: DR
authors.lastname: Mitton
authors.forename: S A
authors.initials: SA
authors.lastname: Larsen
authors.forename: S M
authors.initials: SM
authors.lastname: Yellon
mesh_terms: D000273:Adipose Tissue / Q000187:drug effects / Q000473:pathology; D000375:Aging / Q000378:metabolism* / Q000473:pathology / Q000502:physiology / Q000523:psychology*; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects* / Q000502:physiology; D007328:Insulin / Q000097:blood; D020738:Leptin / Q000097:blood; D008297:Male; D008550:Melatonin / Q000008:administration & dosage* / Q000502:physiology; D010870:Pineal Gland / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D015431:Weight Loss / Q000187:drug effects / Q000502:physiology
chemical_list: D007328:Insulin; D020738:Leptin; D008550:Melatonin