Antioxidant activity of resveratrol in endotoxin-stimulated blood platelets.
Resveratrol (3,4',5-trihydroxystilbene) is a natural molecule with antioxidant action. It is also considered to be a molecule with antiplatelet, anticancer and anti-inflammatory action. The effects of trans-resveratrol on the reactive oxygen species (ROS) generation and thiobarbituric acid-reactive substances (TBARS) in blood platelets induced by endotoxin (lipopolysaccharide, LPS) or thrombin were studied in vitro. The production of superoxide radicals (O2.-) and other reactive oxygen species (H2O2, singlet oxygen, and organic radicals) in the presence of resveratrol was measured by a chemiluminescence method in resting blood platelets and platelets stimulated by LPS (0.3 microg/ 10(8) platelets) or thrombin (2.5 U/10(8) platelets). We have shown that resveratrol (6.25-100 microg/ml) inhibits chemiluminescence and generation of O2.- in blood platelets. It has an inhibitory effect on the production of ROS and TBARS in platelets caused by LPS or thrombin.
Olas
B
B
Department of General Biochemistry, Institute of Biochemistry, University of Lódź, Poland.
Wachowicz
B
B
Saluk-Juszczak
J
J
Zieliński
T
T
Kaca
W
W
Buczyński
A
A
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001792:Blood Platelets / Q000187:drug effects* / Q000378:metabolism; D004731:Endotoxins / Q000633:toxicity; D006490:Hemostatics / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000633:toxicity*; D008163:Luminescent Measurements; D017382:Reactive Oxygen Species / Q000378:metabolism*; D012044:Regression Analysis; D000077185:Resveratrol; D013267:Stilbenes / Q000737:chemistry / Q000494:pharmacology*; D013481:Superoxides / Q000378:metabolism; D013552:Swine; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D013917:Thrombin / Q000494:pharmacology
D000975:Antioxidants; D004731:Endotoxins; D006490:Hemostatics; D008070:Lipopolysaccharides; D017382:Reactive Oxygen Species; D013267:Stilbenes; D017392:Thiobarbituric Acid Reactive Substances; D013481:Superoxides; D013917:Thrombin; D000077185:Resveratrol