Possible roles of beta-catenin in evagination of the optic primordium in rat embryos.
The roles of beta-catenin in evagination of the optic primordium in rat embryos were studied using immunostaining. High levels of beta-catenin appeared transiently in the evaginating optic primordium. Evagination of the optic primordium was suppressed in embryos treated with LiCl. In deficient optic vesicles of these embryos, accumulation of beta-catenin was decreased. Deficient optic vesicles also showed suppression of cyclin D1 accumulation and bromodeoxyuridine incorporation, no break in the deposition of laminin and type IV collagen at the basement membrane (BM) and prevention of the change in distribution of microtubules and microfilaments. These results suggest that beta-catenin regulates cell proliferation, breakdown of BM and changes in cell shape in the evaginating optic primordium to cause optic vesicle formation.
Matsuda
M
M
Department of Embryology, Institute for Developmental Research, Aichi Human Service Center, Kamiya-cho, Kasugai, Aichi 480-0392, Japan. matsuda@inst-hsc.pref.aichi.jp
Keino
H
H
D008841:Actin Cytoskeleton / Q000737:chemistry / Q000648:ultrastructure; D000818:Animals; D001973:Bromodeoxyuridine / Q000378:metabolism; D024141:Collagen Type IV / Q000032:analysis; D019938:Cyclin D1 / Q000378:metabolism; D003598:Cytoskeletal Proteins / Q000032:analysis / Q000502:physiology*; D004622:Embryo, Mammalian / Q000187:drug effects / Q000502:physiology*; D004628:Embryonic Structures / Q000737:chemistry / Q000166:cytology; D005123:Eye / Q000737:chemistry / Q000196:embryology* / Q000378:metabolism; D005136:Eye Proteins / Q000032:analysis; D005260:Female; D018398:Homeodomain Proteins / Q000032:analysis; D007150:Immunohistochemistry; D007797:Laminin / Q000032:analysis; D018021:Lithium Chloride / Q000494:pharmacology; D008297:Male; D008870:Microtubules / Q000737:chemistry / Q000648:ultrastructure; D000071841:PAX6 Transcription Factor; D051761:Paired Box Transcription Factors; D051381:Rats; D017207:Rats, Sprague-Dawley; D012097:Repressor Proteins; D015534:Trans-Activators; D051176:beta Catenin
D024141:Collagen Type IV; C495268:Ctnnb1 protein, rat; D003598:Cytoskeletal Proteins; D005136:Eye Proteins; D018398:Homeodomain Proteins; D007797:Laminin; D000071841:PAX6 Transcription Factor; C000605730:PAX6 protein, human; D051761:Paired Box Transcription Factors; C000605733:Pax6 protein, rat; D012097:Repressor Proteins; D015534:Trans-Activators; D051176:beta Catenin; D019938:Cyclin D1; D001973:Bromodeoxyuridine; D018021:Lithium Chloride