title: TMC-69, a new antitumor antibiotic with Cdc25A inhibitory activity, produced by Chrysosporium sp. TC1068. Taxonomy, fermentation and biological activities.
abstract: A new antibiotic designated TMC-69 has been isolated from the fermentation broth of a fungal strain Chrysosporium sp. TC 1068. TMC-69 exhibited moderate in vitro cytotoxic activity. TMC-69-6H, a derivative of TMC-69 prepared by hydrogenation, possessed more potent in vitro cytotoxicity than TMC-69, and exhibited in vivo antitumor activity against murine P388 leukemia and B16 melanoma. TMC-69-6H was found to specifically inhibit Cdc25A and B phosphatases.
authors.affiliation: Discovery Research Laboratory, Tanabe Seiyaku Co, Ltd, Saitama, Japan. hiranon@tanabe.co.jp
authors.forename: N
authors.initials: N
authors.lastname: Hirano
authors.forename: J
authors.initials: J
authors.lastname: Kohno
authors.forename: S
authors.initials: S
authors.lastname: Tsunoda
authors.forename: M
authors.initials: M
authors.lastname: Nishio
authors.forename: N
authors.initials: N
authors.lastname: Kishi
authors.forename: T
authors.initials: T
authors.lastname: Okuda
authors.forename: K
authors.initials: K
authors.lastname: Kawano
authors.forename: S
authors.initials: S
authors.lastname: Komatsubara
authors.forename: N
authors.initials: N
authors.lastname: Nakanishi
mesh_terms: D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D001681:Biological Assay; D002455:Cell Division / Q000187:drug effects; D002912:Chrysosporium / Q000254:growth & development / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D006801:Humans; D006865:Hydrogenation; D020128:Inhibitory Concentration 50; D007941:Leukemia P388 / Q000188:drug therapy; D008546:Melanoma, Experimental / Q000188:drug therapy; D051379:Mice; D011714:Pyrans / Q000494:pharmacology*; D014407:Tumor Cells, Cultured; D020687:cdc25 Phosphatases / Q000037:antagonists & inhibitors* / Q000378:metabolism
chemical_list: D000903:Antibiotics, Antineoplastic; D004791:Enzyme Inhibitors; D011714:Pyrans; C433006:TMC-69; C433007:TMC-69-6H; C517004:CDC25A protein, human; C517005:Cdc25a protein, mouse; D020687:cdc25 Phosphatases