title: Expression of truncated FHIT transcripts in cervical cancers and in normal human cells.
abstract: To analyse FHIT transcription patterns in cervical cancer, a series of primary cervical tumors and normal control samples were studied using RT-PCR. Full length and truncated FHIT transcripts were detectable in all samples tested. Interestingly, the expression of truncated FHIT transcripts by primary epithelial cells in vitro was associated with confluency. The breakpoints of most transcript deletions coincided with genuine splice site sequences, suggesting that they resulted from alternative splicing. These findings demonstrate that truncated FHIT transcripts are commonly detected in both normal and tumor tissues, and suggest that these altered transcripts are not causally related to tumorigenesis in cervical cancer.
authors.affiliation: Fred Hutchinson Cancer Research Center, Cancer Biology Program, 1100 Fairview Avenue N., P.O. Box 19024, Seattle, Washington, WA 98109-1024, USA. cmatthew@fhcrc.org
authors.forename: C P
authors.initials: CP
authors.lastname: Matthews
authors.forename: K
authors.initials: K
authors.lastname: Shera
authors.forename: N
authors.initials: N
authors.lastname: Kiviat
authors.forename: J K
authors.initials: JK
authors.lastname: McDougall
mesh_terms: D017766:Acid Anhydride Hydrolases; D017398:Alternative Splicing; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000378:metabolism; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics; D056827:Endosomal Sorting Complexes Required for Transport; D005260:Female; D006801:Humans; D007041:Hypoxanthine Phosphoribosyltransferase / Q000096:biosynthesis / Q000235:genetics; D009363:Neoplasm Proteins; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis; D012334:RNA, Neoplasm / Q000096:biosynthesis; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics; D014158:Transcription, Genetic; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000378:metabolism; D000099177:Tsg101 Protein; D000099170:Bis(5'-adenosyl)triphosphatase
chemical_list: D017766:Acid Anhydride Hydrolases; D004268:DNA-Binding Proteins; D056827:Endosomal Sorting Complexes Required for Transport; D007041:Hypoxanthine Phosphoribosyltransferase; D009363:Neoplasm Proteins; D011506:Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D014157:Transcription Factors; D000099177:Tsg101 Protein; D000099170:Bis(5'-adenosyl)triphosphatase