A packed-bed fungal bioreactor for the continuous decolourisation of azo-dyes (Orange II).
The degradation of an azo dye, Orange II, by immobilised Phanerochaete chrysosporium in a continuous packed bed bioreactor for periods longer than 30 days has been carried out. Nearly complete decolourisation (>95%) was achieved when working at a high dye load rate of 0.2 g x l(-1) x d(-1), a temperature of 37 degrees C, a hydraulic retention time (HRT) of 24 h and applying oxygen gas in a pulsed flow. These conditions allowed Manganese peroxidase (MnP) production and the subsequently Orange II decolourisation. A correlation between residual MnP activity in the effluent and decolourisation was established. Apparently, for decolourisation to be effective, a minimum MnP activity was required, no substantial increase in efficiency at MnP activities higher than 10 U x 1(-1) was observed. The treatment caused, the breakdown of the chromophoric group as well as the cleavage of the aromatic ring.
Mielgo
I
I
Department of Chemical Engineering, Institute of Technology, University of Santiago de Compostela, E-15706 Santiago de Compostela, Gazila, Spain.
Moreira
M T
MT
Feijoo
G
G
Lema
J M
JM
D001391:Azo Compounds / Q000378:metabolism*; D001557:Benzenesulfonates / Q000378:metabolism*; D019149:Bioreactors; D003116:Color; D004396:Coloring Agents / Q000378:metabolism*; D007700:Kinetics; D010100:Oxygen / Q000378:metabolism; D020075:Phanerochaete / Q000378:metabolism*; D013696:Temperature
D001391:Azo Compounds; D001557:Benzenesulfonates; D004396:Coloring Agents; C014722:2-naphthol orange; D010100:Oxygen