Human alpha-and beta-defensin immunoreactivity in oral mucoepidermoid carcinomas.
The purpose of this study was to demonstrate the immunohistochemical localization and distribution of human alpha- and beta-defensins, peptides with antimicrobial activity, in oral mucoepidermoid carcinoma tissue. Tissue samples were embedded in paraffin and alpha- and beta-defensins were immunostained by the streptavidin-biotin coupled peroxidase method. Cancer cells that constituted the ducts, as well as neutrophils, were positively immunostained with the anti-alpha-defensin antibody (HNPs). On the other hand, epidermoid cells and intermediate cells were intensely stained with the anti-beta-defensin-2 (HBD-2) antibody. Mucous-secreting cells were clearly not immunostained with the anti-HBD-2 antibody. The epithelial hyperplasia region adjacent to the tumor tissues was also positively immunostained with the anti-HBD-2 antibody.
Mizukawa
N
N
Department of Oral and Maxillofacial Surgery 1, Okayama University Dental School, Japan.
Sawaki
K
K
Nagatsuka
H
H
Kamio
M
M
Yamachika
E
E
Fukunaga
J
J
Ueno
T
T
Takagi
S
S
Sugahara
T
T
D018277:Carcinoma, Mucoepidermoid / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D009062:Mouth Neoplasms / Q000276:immunology / Q000378:metabolism*; D016612:Paraffin Embedding; D013194:Staining and Labeling / Q000379:methods; D023084:alpha-Defensins / Q000276:immunology / Q000378:metabolism*; D023083:beta-Defensins / Q000276:immunology / Q000378:metabolism*
D023084:alpha-Defensins; D023083:beta-Defensins