Isolation, nucleotide sequence and expression of a cDNA encoding feline granulocyte colony-stimulating factor.
A cDNA encoding feline granulocyte colony stimulating factor (fG-CSF) was cloned from alveolar macrophages using the reverse transcriptase-polymerase chain reaction. The cDNA is 949 bp in length and encodes a predicted mature protein of 174 amino acids. Recombinant fG-CSF was expressed as a glutathione S-transferase fusion and purified by affinity chromatography. Biological activity of the recombinant protein was demonstrated using the murine myeloblastic cell line GNFS-60, which showed an ED50 for fG-CSF of approximately 2 ng/ml.
Dunham
S P
SP
Department of Veterinary Pathology, University of Glasgow, Bearsden Road, G61 1QH, UK. s.dunham@vet.gla.ac.uk
Onions
D E
DE
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002415:Cats; D002417:Cattle; D002460:Cell Line; D002845:Chromatography; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism*; D005982:Glutathione Transferase / Q000378:metabolism; D016179:Granulocyte Colony-Stimulating Factor / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics*; D006801:Humans; D016676:Macrophages, Alveolar / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D012756:Sheep
D018076:DNA, Complementary; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor; D005982:Glutathione Transferase