[Guiding-evaluation of immunomodulating activity of the Liuwei Dihuang Decoction during the stepwise fractionation].
OBJECTIVE
To conduct the activity evaluation for immunomodulating components from the Liuwei Dihuang Decoction (LWDHD).


METHODS
Cyclophosphamide (Cy)-treated mice were used as the immunodeficient model and the antibody production response in plaque-forming cell (PFC) assay was employed as the activity-evaluating parameter. Stepwise fractionation was guided by activity-evaluation.


RESULTS
Fr1, the ethanol-soluble fraction, and Fr2, the ethanol insoluble fraction of LWDHD, both significantly improved the antibody production response in Cy-treated mice, in which Fr2 showed stronger activity than that of Fr1. Fr2 was further fractionated. Fr2-CA4, one of the fractions obtained by active carbon chromatography from Fr2, exhibited the strongest activity in comparison with other fractions.


CONCLUSIONS
CA4, which is mainly composed of acidic polysaccharides, is the main immunomodulating active fraction contained in the ethanol-insoluble fraction of LWDHD.
Nie
W
W
Beijing Institute of Pharmacology and Toxicology, Beijing 100850.
Zhang
Y
Y
Ru
X
X
D000276:Adjuvants, Immunologic / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D000917:Antibody Formation; D003520:Cyclophosphamide; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology*; D005260:Female; D016867:Immunocompromised Host / Q000276:immunology*; D051379:Mice; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D011897:Random Allocation; D013154:Spleen / Q000166:cytology / Q000276:immunology
D000276:Adjuvants, Immunologic; D004365:Drugs, Chinese Herbal; D011134:Polysaccharides; D003520:Cyclophosphamide