A new in vitro model for the study of pig-to-human vascular hyperacute rejection.
Studies on vascular hyperacute xenograft rejection (HAR) are usually conducted in vitro on cultured endothelial cells (EC) exposed to human serum, in complex whole organ perfusion models using heparinized blood or in vivo models. Here we describe a new model allowing perfusion of pig vessels with human whole blood without anticoagulants. Segments of the porcine iliac artery were connected to circular polyvinyl chloride (PVC) tubing, whose inner surface was conjugated with immobilized heparin. The vessels were perfused with 7 to 8 ml of fresh, non-anticoagulated human blood by rocking of the tubing device for 5, 15 or 60 min in an incubator at 37 degrees C. Human iliac arteries (n = 4) were perfused with fresh human ABO-compatible blood as controls. Perfusion of human vessels resulted in changes in the blood and plasma parameters similar to those in the PVC control loop. Overall, perfusion of the porcine vessels generated high levels of C3a, sC5b-9 and thrombin-anti-thrombin (TAT). Platelet consumption was near total (97.2 +/- 1.2%; "high" responders) in six of 13 vessels perfused and only moderate (55.8 +/- 9.9%; "low" responders) in the remaining seven vessels. The "high" responder vessel group showed a significantly higher platelet reduction, neutrophil loss and monocyte consumption and higher C3a and TAT factor at 60 min compared with the human vessels. The "low" responder porcine vessel group also generated significantly higher TAT levels at 60 min compared with the human vessels, but lower levels compared with the "high" responder porcine vessel group. Immunohistochemical examination of perfused porcine vessels revealed binding of human IgM, IgG, IgA, C1q, C3, fibrin and platelets at 5 min. The binding of these proteins was even stronger at 15 and 60 min, and at 60 min C9 could also be detected. Addition of soluble complement receptor 1 (sCR1) to the blood resulted in a significant reduction in C3a and sC5b-9 (P = 0.046 and P = 0.046, respectively). However, sCR1 did not reduce C1q, C3c or C5 staining, but did abolish C9 binding to the endothelium. In conclusion, in vitro perfusion of porcine vessel segments with non-heparinized, fresh human blood triggered events characterizing HAR. The small quantity of blood and xenogenic tissue that is needed makes this model ideal for investigations of the mechanisms and treatments of rejections associated with xenogeneic pig-to-human xenotransplantation.
Bennet
W
W
Department of Transplantation Surgery, Karolinska Institute, Huddinge Hospital, Huddinge, Sweden.
Sundberg
B
B
Elgue
G
G
Larsson
R
R
Korsgren
O
O
Nilsson
B
B
D000017:ABO Blood-Group System / Q000276:immunology; D000818:Animals; D000990:Antithrombin III / Q000032:analysis; D001769:Blood; D001772:Blood Cell Count; D001792:Blood Platelets / Q000276:immunology / Q000502:physiology; D002478:Cells, Cultured; D015926:Complement C3a / Q000032:analysis; D015938:Complement Membrane Attack Complex / Q000032:analysis; D004730:Endothelium, Vascular / Q000276:immunology*; D006084:Graft Rejection / Q000276:immunology*; D006801:Humans; D007083:Iliac Artery; D018448:Models, Immunological; D010447:Peptide Hydrolases / Q000032:analysis; D010477:Perfusion; D011143:Polyvinyl Chloride; D013552:Swine; D014183:Transplantation, Heterologous / Q000276:immunology*
D000017:ABO Blood-Group System; D015938:Complement Membrane Attack Complex; C046193:antithrombin III-protease complex; D015926:Complement C3a; D000990:Antithrombin III; D011143:Polyvinyl Chloride; D010447:Peptide Hydrolases