Investigations into the interaction between tumor-inhibiting ruthenium(III) complexes and nucleotides by capillary electrophoresis.
Ruthenium(III) complexes of the general formula HL[RuCl4L2], with two trans-standing heterocyclic ligands L bound to ruthenium via nitrogen, show remarkable activity in different tumor models. To obtain a deeper insight into the mode of action of this class of anticancer compounds, we investigated the interaction of HIm trans-[RuC14(im)2] (im, imidazole) and HInd trans-[RuCl4(ind)2] (ind, indazole) with all four nucleoside monophosphates in buffered solution by means of capillary electrophoresis. A preference for GMP- and AMP-coordination was found. A decrease of the pH resulted in a significantly increased amount of bound nucleotide. This feature seems to be interesting with regard to the lower pH values in solid tumors.
Küng
A
A
Institute of Inorganic Chemistry, University of Vienna, Austria.
Pieper
T
T
Keppler
B K
BK
D000249:Adenosine Monophosphate / Q000737:chemistry; D000970:Antineoplastic Agents / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006157:Guanosine Monophosphate / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D009711:Nucleotides / Q000737:chemistry*; D017975:Ruthenium Compounds / Q000737:chemistry*
D000970:Antineoplastic Agents; D009711:Nucleotides; D017975:Ruthenium Compounds; D000249:Adenosine Monophosphate; D006157:Guanosine Monophosphate