Regulation of dendritic spine morphology and synaptic function by Shank and Homer.
The Shank family of proteins interacts with NMDA receptor and metabotropic glutamate receptor complexes in the postsynaptic density (PSD). Targeted to the PSD by a PDZ-dependent mechanism, Shank promotes the maturation of dendritic spines and the enlargement of spine heads via its ability to recruit Homer to postsynaptic sites. Shank and Homer cooperate to induce accumulation of IP3 receptors in dendritic spines and formation of putative multisynapse spines. In addition, postsynaptic expression of Shank enhances presynaptic function, as measured by increased minifrequency and FM4-64 uptake. These data suggest a central role for the Shank scaffold in the structural and functional organization of the dendritic spine and synaptic junction.
Sala
C
C
Howard Hughes Medical Institute and, Department of Neurobiology, Massachusetts General Hospital and, Harvard Medical School, 02114, Boston, MA, USA.
Piëch
V
V
Wilson
N R
NR
Passafaro
M
M
Liu
G
G
Sheng
M
M
D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000502:physiology*; D002478:Cells, Cultured; D003712:Dendrites / Q000502:physiology* / Q000648:ultrastructure*; D054637:Dual-Specificity Phosphatases; D004622:Embryo, Mammalian; D049452:Green Fluorescent Proteins; D006624:Hippocampus / Q000166:cytology / Q000502:physiology*; D000072233:Homer Scaffolding Proteins; D008164:Luminescent Proteins / Q000032:analysis / Q000235:genetics; D008564:Membrane Potentials / Q000502:physiology; D009419:Nerve Tissue Proteins / Q000032:analysis; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D009479:Neuropeptides / Q000235:genetics / Q000502:physiology*; D010749:Phosphoprotein Phosphatases / Q000032:analysis; D017966:Pyramidal Cells / Q000166:cytology / Q000502:physiology*; D051381:Rats; D011994:Recombinant Proteins / Q000378:metabolism; D013569:Synapses / Q000502:physiology*; D009435:Synaptic Transmission / Q000502:physiology; D014162:Transfection
D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; D000072233:Homer Scaffolding Proteins; D008164:Luminescent Proteins; D009419:Nerve Tissue Proteins; D009479:Neuropeptides; D011994:Recombinant Proteins; C120531:Shank1 protein, rat; C033652:postsynaptic density proteins; D049452:Green Fluorescent Proteins; D010749:Phosphoprotein Phosphatases; C489855:dual specificity phosphatase 12; D054637:Dual-Specificity Phosphatases