Enzymic activities in two populations of purified rat islet beta-cells.
In terms of glucose sensing by pancreatic islet beta-cells, emphasis is currently placed on both the role of glucokinase, with negligible activity of low-Km hexokinase(s), and the prevalence of the oxidative over non-oxidative modality of glycolysis, a situation tentatively attributed, in part at least, to a low activity of lactate dehydrogenase. Conflicting information is available, however, on the activity of both low-Km hexokinase(s) and lactate dehydrogenase in purified beta-cell homogenates. This issue was reinvestigated, therefore, in two populations of purified rat islet beta-cells selected on the basis of their low (betaL) or high (betaH) content in reduced pyridine nucleotides. The size and protein content of betaH cells represented about twice that of betaL cells. Such was also the case for low-Km hexokinase(s), lactate dehydrogenase, mitochondrial FAD-linked glycerophosphate dehydrogenase, glutamate dehydrogenase and glutamate-alanine and glutamate-aspartate transaminases. Whether in betaH or betaL cells, the activity of low-Km hexokinase(s) was at least as high as or higher than that of glucokinase. In both betaH and betaL, the activity of lactate dehydrogenase exceeded that required to catalyze the full reduction of glucose-derived pyruvate to L-lactate, as estimated from the rate of D-glucose phosphorylation under physiological conditions. These findings thus argue against a low expression of either low-Km hexokinase(s) or lactate dehydrogenase as major determinants of the glucose-sensing device in beta-cells.
Sener
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Laboratory of Experimental Medicine, Brussels Free University, B-1070 Brussels, Belgium.
Mercan
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Malaisse
W J
WJ
D000410:Alanine Transaminase / Q000378:metabolism; D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism; D048429:Cell Size; D004305:Dose-Response Relationship, Drug; D004798:Enzymes / Q000378:metabolism*; D005260:Female; D005636:Fructosephosphates / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology; D019298:Glucose-6-Phosphate / Q000494:pharmacology; D005969:Glutamate Dehydrogenase / Q000378:metabolism; D006593:Hexokinase / Q000378:metabolism; D007328:Insulin / Q000378:metabolism; D007515:Islets of Langerhans / Q000166:cytology / Q000201:enzymology* / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar
D004798:Enzymes; D005636:Fructosephosphates; D007328:Insulin; C032284:fructose-1-phosphate; D019298:Glucose-6-Phosphate; D007770:L-Lactate Dehydrogenase; D005969:Glutamate Dehydrogenase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D006593:Hexokinase; D005947:Glucose