title: Human alpha-and beta-defensin immunoreactivity in oral mucoepidermoid carcinomas.
abstract: The purpose of this study was to demonstrate the immunohistochemical localization and distribution of human alpha- and beta-defensins, peptides with antimicrobial activity, in oral mucoepidermoid carcinoma tissue. Tissue samples were embedded in paraffin and alpha- and beta-defensins were immunostained by the streptavidin-biotin coupled peroxidase method. Cancer cells that constituted the ducts, as well as neutrophils, were positively immunostained with the anti-alpha-defensin antibody (HNPs). On the other hand, epidermoid cells and intermediate cells were intensely stained with the anti-beta-defensin-2 (HBD-2) antibody. Mucous-secreting cells were clearly not immunostained with the anti-HBD-2 antibody. The epithelial hyperplasia region adjacent to the tumor tissues was also positively immunostained with the anti-HBD-2 antibody.
authors.affiliation: Department of Oral and Maxillofacial Surgery 1, Okayama University Dental School, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Mizukawa
authors.forename: K
authors.initials: K
authors.lastname: Sawaki
authors.forename: H
authors.initials: H
authors.lastname: Nagatsuka
authors.forename: M
authors.initials: M
authors.lastname: Kamio
authors.forename: E
authors.initials: E
authors.lastname: Yamachika
authors.forename: J
authors.initials: J
authors.lastname: Fukunaga
authors.forename: T
authors.initials: T
authors.lastname: Ueno
authors.forename: S
authors.initials: S
authors.lastname: Takagi
authors.forename: T
authors.initials: T
authors.lastname: Sugahara
mesh_terms: D018277:Carcinoma, Mucoepidermoid / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D009062:Mouth Neoplasms / Q000276:immunology / Q000378:metabolism*; D016612:Paraffin Embedding; D013194:Staining and Labeling / Q000379:methods; D023084:alpha-Defensins / Q000276:immunology / Q000378:metabolism*; D023083:beta-Defensins / Q000276:immunology / Q000378:metabolism*
chemical_list: D023084:alpha-Defensins; D023083:beta-Defensins