title: Effect of sodium hypochlorite and five intracanal medications on Candida albicans in root canals.
abstract: The aim of this study was to evaluate the effect of 1% sodium hypochlorite and five intracanals medications on Candida albicans harvested inside root canals. The contaminated canals were irrigated with sterile saline solution and then treated as follows: (i) filled with Calen paste (calcium hydroxide/ glycol polyethylene paste); (ii) filled with camphorated paramonochloro phenol (CPMC); (iii) filled with 2% iodine-iodate solution; (iv) filled with tricresol formalin; (v) filled with Calen and CPMC pastes; (vi) irrigation with 1% sodium hypochlorite and filled with no intracanal medication; and (vii) no intracanal medication was used. Canal access and the apical foramen were then sealed with Cavit and the roots were stored in a humid chamber at 37 +/- 1 degree C for 14 days. The canals were reinstrumented and irrigated with sterile saline solution. Sterile paper points were used to transfer the root canal contents to test tubes containing sterile saline solution. Part of the suspension was harvested in Sabouraud dextrose agar with chloramphenicol and incubated at 37 +/- 1 degree C for 48 h. CPMC was effective in 100% of the samples followed in decreasing order of effectiveness by calcium hydroxide with CPMC (70% effective), 1% sodium hypochlorite (70% effective) (p < 0.05), tricresol formalin (60% effective), 2% iodine-iodate solution (50% effective), calcium hydroxide paste (30% effective), and saline + no intracanal medication.
authors.affiliation: Department of Endodontics, School of Dentistry, Paulista State University (UNESP), SP, Brazil.
authors.forename: M C
authors.initials: MC
authors.lastname: Valera
authors.forename: J
authors.initials: J
authors.lastname: de Moraes Rego
authors.forename: A O
authors.initials: AO
authors.lastname: Jorge
mesh_terms: D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D002126:Calcium Hydroxide / Q000494:pharmacology; D002133:Calcium Sulfate / Q000494:pharmacology; D002164:Camphor / Q000494:pharmacology; D002176:Candida albicans / Q000187:drug effects*; D002733:Chlorophenols / Q000494:pharmacology; D003408:Cresols / Q000494:pharmacology; D003738:Dental Cements; D003786:Dental Pulp Cavity / Q000382:microbiology*; D004338:Drug Combinations; D005557:Formaldehyde / Q000031:analogs & derivatives* / Q000494:pharmacology; D006801:Humans; D006813:Humidity; D007452:Iodates / Q000494:pharmacology; D017613:Iodine Compounds / Q000494:pharmacology; D011092:Polyethylene Glycols / Q000494:pharmacology; D011145:Polyvinyls / Q000494:pharmacology; D012387:Root Canal Filling Materials / Q000494:pharmacology; D012388:Root Canal Irrigants / Q000494:pharmacology*; D012973:Sodium Hypochlorite / Q000494:pharmacology*; D013223:Statistics as Topic; D013501:Surface-Active Agents / Q000494:pharmacology; D013696:Temperature; D015034:Zinc Oxide / Q000494:pharmacology
chemical_list: D000891:Anti-Infective Agents, Local; D002733:Chlorophenols; D003408:Cresols; D003738:Dental Cements; D004338:Drug Combinations; D007452:Iodates; D017613:Iodine Compounds; D011145:Polyvinyls; D012387:Root Canal Filling Materials; D012388:Root Canal Irrigants; D013501:Surface-Active Agents; C420643:calen; D005557:Formaldehyde; D011092:Polyethylene Glycols; C020872:Cavit; D002164:Camphor; C029804:camphorated parachlorophenol; C011372:tricresolformalin; D012973:Sodium Hypochlorite; D002126:Calcium Hydroxide; D015034:Zinc Oxide; D002133:Calcium Sulfate