[A comparative study of application of PCR technique and Porphyromonas gingivalis nucleic acid probe in clinical examination of apical periodontitis].
By PCR technique and Pg nucleic acid probe, the authors investigated the Pg distribution in root canal samples from patients with clinical apical periodontitis and made a comparative study of the two methods. The results showed that: 1. Pg positive rate of apical periodontitis samples by PCR examination was 74% and that of by nucleic acid probe examination was 76%, and their total coincidence rate reached 94%; 2. between comparative groups there was no significant difference (P > 0.05) in all data by two examination methods; 3. Pg was closely related with clinical symptoms of apical periodontitis such as spontaneous pain, percussion pain, fetidity and periapical abscess. This indicates that the two methods are accurate, quick and convenient and can both be used in direct examination of clinical samples.
Liu
L
L
School of Stomatology, the Fourth Military Medical University, China.
Shi
J
J
Ji
C
C
D000293:Adolescent; D000328:Adult; D016866:Bacteroidaceae Infections / Q000382:microbiology*; D002648:Child; D003786:Dental Pulp Cavity / Q000382:microbiology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D015341:Nucleic Acid Probes; D010518:Periodontitis / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D016966:Porphyromonas gingivalis / Q000302:isolation & purification*
D015341:Nucleic Acid Probes