[Determination of monocyte chemotactic protein-1 in cultured endometriotic cells].
OBJECTIVE
To investigate the clinical significance of monocyte chemotactic protein-1 (MCP-1) produced by endometriotic tissues.


METHODS
Expressions of MCP-1 mRNA and MCP-1 protein were determined by dot blot analysis, immunohistochemical method [streptavidin biotin-peroxidase complex (SABC)] and enzyme linked immunosorbent assay (ELISA) methods in cultured endometriotic cells with median (controls) or with interleukin-1 beta(IL-1 beta) 2 ng/ml (IL-1 beta group), and tumor necrosis factor-alpha(TNF-alpha) 20 mg/ml(TNF-alpha group) respectively. The endometriotic tissues were sourced from 15 patients with endometriosis. Meanwhile, the MCP-1 protein content of cultured supernatent was also measured.


RESULTS
After exposured to IL-1 beta or TNF-alpha, the expression of MCP-1 mRNA and MEP-1 protein in the endometriotic cells were significantly higher than that in the control group (P < 0.01, P < 0.05 respectively); so was the MCP-1 protein content of supernatent.


CONCLUSIONS
IL-1 beta and TNF-alpha can up-regulate the expression of MCP-1 in endometriotic cells, which may be related to the development of endometriosis.
Gao
Y
Y
Union Hospital, Tongji Medical University, Wuhan 430022.
Luo
L
L
He
F
F
D000328:Adult; D002478:Cells, Cultured; D018932:Chemokine CCL2 / Q000032:analysis* / Q000096:biosynthesis / Q000235:genetics; D004715:Endometriosis / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D007375:Interleukin-1 / Q000378:metabolism; D010049:Ovarian Diseases / Q000378:metabolism* / Q000473:pathology; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D018932:Chemokine CCL2; D007375:Interleukin-1; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha