Determination of LSD in urine with high-performance liquid chromatography--mass spectrometry.
A rapid, specific, and sensitive high-performance liquid chromatography/mass spectrometry method has been developed for routine determination of lysergic acid diethylamide (LSD) in urine. It includes sample purification by extraction into an organic solvent and back-extraction to an acetate buffer, reversed-phase high-performance liquid chromatography, and detection with a single quadrupole mass spectrometer equipped with an atmospheric pressure ionization electrospray interface. Trideuterated LSD was used as internal standard. The limit of detection was 0.02 ng/mL and the calibration curve was linear from 0.05 to 10 ng/mL. Within-and between-day coefficients of variation were 3.5% and 4.0% respectively and extraction recovery was 91%.
Bodin
K
K
Department of Clinical Pharmacology, Huddinge University Hospital, Huddinge, Sweden.
Svensson
J O
JO
D002853:Chromatography, Liquid / Q000379:methods*; D016903:Drug Monitoring; D006213:Hallucinogens / Q000652:urine*; D006801:Humans; D008238:Lysergic Acid Diethylamide / Q000652:urine*; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D015813:Substance Abuse Detection / Q000379:methods*
D006213:Hallucinogens; D008238:Lysergic Acid Diethylamide