title: Accelerated ageing changes in the choroid plexus of a case with multiple mitochondrial DNA deletions.
abstract: Mitochondrial abnormalities, in particular the accumulation of mitochondrial DNA mutations, have been proposed as a potential cause of normal ageing. One group of patients with mtDNA disorders have a nuclear DNA defect which accelerates the chronological accumulation of mitochondrial DNA mutations. These patients provide an ideal means of investigating whether accelerated mitochondrial DNA defects can cause accelerated ageing pathology. The choroid plexus demonstrates a robust accumulation of pathological changes, in the form of Biondi bodies, with normal ageing. We have therefore examined the choroid plexus of a case with multiple mitochondrial DNA deletions for evidence of accelerated ageing and compared it with two cases with point mutation mitochondrial DNA disorders and several age-matched and elderly controls with and without clinical and neuropathological evidence of neurodegenerative disease. We also demonstrate that the choroid plexus of the mitochondrial DNA cases contain cells with levels of mitochondrial DNA mutation sufficient to cause a biochemical deficiency in the oxidative phosphorylation pathway. As previously reported, both cases with point mutation mitochondrial DNA disorders exhibit a characteristic oncocytic type transformation of the choroidal epithelial cells. However, in the case with multiple mitochondrial DNA deletions we demonstrate pathological changes in choroid plexus that are strongly suggestive of accelerated ageing. We believe that this finding supports the theory that the accumulation of mitochondrial DNA mutations can lead to pathological changes typical of ageing cells.
authors.affiliation: Department of Neurology, The Medical School, University of Newcastle upon Tyne, Newcastle upon Tyne, NE2 4HH, UK. d.a.Cottrell@newcastle.ac.uk
authors.forename: D A
authors.initials: DA
authors.lastname: Cottrell
authors.forename: P G
authors.initials: PG
authors.lastname: Ince
authors.forename: T M
authors.initials: TM
authors.lastname: Wardell
authors.forename: D M
authors.initials: DM
authors.lastname: Turnbull
authors.forename: M A
authors.initials: MA
authors.lastname: Johnson
mesh_terms: D000328:Adult; D000375:Aging / Q000502:physiology*; D002831:Choroid Plexus / Q000473:pathology*; D004272:DNA, Mitochondrial / Q000235:genetics*; D005260:Female; D017353:Gene Deletion; D006801:Humans; D008297:Male; D008875:Middle Aged; D017240:Mitochondrial Myopathies / Q000235:genetics / Q000473:pathology; D017354:Point Mutation; D013997:Time Factors
chemical_list: D004272:DNA, Mitochondrial