title: Analysis of Tag1-like elements in Arabidopsis thaliana and their distribution in other plants.
abstract: Analysis of genomic DNA of Arabidopsis Columbia (Col.) ecotype using a transposon Tag1-specific primer showed the presence of Tag1 homologues which was confirmed by Southern hybridization with a Tag1 probe. Further analysis showed that the homologue, 0.75 kb in length, had inverted repeats at both ends, 8-bp duplicated sequences at the site at which it is located and about 80% homology with Tag1, and was randomly distributed in the Arabidopsis genome. Based on these results, we concluded that these elements are non-autonomous variants of Tag1 and we termed this element sTag1. Using the polymerase chain reaction fragment hybridization technique, we found the distribution of such homologues in other plant species.
authors.affiliation: Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, Ueda, Nagano, Japan.
authors.forename: P C
authors.initials: PC
authors.lastname: Shankar
authors.forename: S
authors.initials: S
authors.lastname: Ito
authors.forename: M
authors.initials: M
authors.lastname: Kato
authors.forename: M
authors.initials: M
authors.lastname: Matsui
authors.forename: R
authors.initials: R
authors.lastname: Kodaira
authors.forename: N
authors.initials: N
authors.lastname: Hayashida
authors.forename: M
authors.initials: M
authors.lastname: Okazaki
mesh_terms: D017360:Arabidopsis / Q000235:genetics*; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004251:DNA Transposable Elements / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics*; D018745:Genome, Plant; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid
chemical_list: D004251:DNA Transposable Elements; D018744:DNA, Plant