Proliferation stage-dependent expression of DNA methyltransferase (Dnmt1) in mouse small intestine.
In cultured cells, the maintenance-type DNA methyltransferase (Dnmt1) is highly expressed during the proliferation stage. In the present study, we detected significant expression of Dnmt1 protein in the nuclear fraction of mouse small intestine. From its mobility in SDS polyacrylamide gel electrophoresis and the specific antibodies against the somatic cell-type Dnmt1, Dnmt1 was determined as a somatic cell type. Immunofluorescence study revealed that the Dnmt1 was highly expressed in the proliferating stem cells in crypts, and was localized in the nuclei. The present results indicate that the expression of Dnmt1 in vivo is also under the control of cell proliferation as in cultured cells.
Suetake
L
L
Institute for Protein Research, Osaka University, Suita, Japan. suetaka@protein.osaka-u.ac.jp
Shi
L
L
Watanabe
D
D
Nakamura
M
M
Tajima
S
S
D000818:Animals; D019556:COS Cells; D002455:Cell Division; D002522:Chlorocebus aethiops; D000076123:DNA (Cytosine-5-)-Methyltransferase 1; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000096:biosynthesis / Q000235:genetics*; D019084:Fluorescent Antibody Technique, Indirect; D015870:Gene Expression; D007421:Intestine, Small / Q000201:enzymology*; D051379:Mice
D000076123:DNA (Cytosine-5-)-Methyltransferase 1; D004248:DNA (Cytosine-5-)-Methyltransferases; C000619381:Dnmt1 protein, mouse