title: [Effects of composite blood-activating decoction on bone marrow microenvironment in mice of immune-induced aplastic anemia].
abstract: OBJECTIVE
  To explore the mechanism of elevating efficacy for aplastic anemia (AA) by using blood-activating and stasis-eliminating drugs.
  
  
  METHODS
  Immune-induced aplastic anemia model was established. Each mouse was gastrogavaged by 0.2 ml 100% composite blood-activating decoction (CBAD) twice a day. On the 10th day, the bone marrow histology, CFU-F, adhesive function of the cultured stromal cell layer, bone marrow PO2 were observed.
  
  
  RESULTS
  In CBAD group, the WBC count, bone marrow karyocytes, bone marrow hematopoietic tissue volume, CFU-F count were significantly higher than those in AA group (P < 0.01). Moreover, the adhesive function of stromal cells and bone marrow PO2 recovered to normal level.
  
  
  CONCLUSIONS
  The commonly used composite blood-activating decoction could promote the recovery and oxygen-supply of bone marrow microenvironment in AA mice, and improved the bone marrow hematopoiesis.
authors.affiliation: Tongji Hospital, Tongji Medical University, Wuhan 430030.
authors.forename: Y
authors.initials: Y
authors.lastname: Shu
authors.forename: H
authors.initials: H
authors.lastname: Sun
authors.forename: W
authors.initials: W
authors.lastname: Liu
mesh_terms: D000741:Anemia, Aplastic / Q000188:drug therapy / Q000473:pathology; D000818:Animals; D001853:Bone Marrow / Q000473:pathology*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology / Q000627:therapeutic use; D005260:Female; D006412:Hematopoietic Stem Cells / Q000473:pathology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008811:Mice, Inbred DBA; D011897:Random Allocation
chemical_list: D004365:Drugs, Chinese Herbal