title: Learning behaviour and cerebral protein kinase C, antioxidant status, lipid composition in senescence-accelerated mouse: influence of a phosphatidylcholine-vitamin B12 diet.
abstract: Our objective was to determine whether dietary supplementation with phosphatidylcholine (PC) plus vitamin B12 could afford beneficial effects on biochemical and biophysical events in the brain of senescence-accelerated mouse (SAM) substrain SAMP8. We measured learning behaviour, hippocampal protein kinase C (PKC) activity, cerebral antioxidant status, phospholipid composition and fatty acid composition in 6-month-old SAMP8 and in age-matched controls (SAM substrain SAMR1). In comparison with SAMR1, SAMP8 showed a significant elevation in total grading score of senescence and a significant decline in acquisition SAMP8 had a lower hippocampal PKC activity and cerebral PKC-beta mRNA abundance than SAMR1. SAMP8 had increased cerebral lipid peroxide levels and proportion of sphingomyelin, and a lower proportion of 20 : 4n-6 and 22 : 6n-3 in cerebral phosphtidylethanolamine than SAMR1. SAMP8 fed the PC combined with vitamin B12 diet had an increased PKC activity and a higher proportion of 22 : 6n-3 than SAMP8 fed the control diet. These results indicate the potential benefit of PC combined with vitamin B12 as a dietary supplement.
authors.affiliation: Laboratory of Nutrition Chemistry, Division of Bioresource and Bioenvironmental Sciences, Graduate School, Kyushu University, Fukuoka 812-8581, Japan.
authors.forename: M C
authors.initials: MC
authors.lastname: Hung
authors.forename: K
authors.initials: K
authors.lastname: Shibasaki
authors.forename: R
authors.initials: R
authors.lastname: Yoshida
authors.forename: M
authors.initials: M
authors.lastname: Sato
authors.forename: K
authors.initials: K
authors.lastname: Imaizumi
mesh_terms: D000375:Aging / Q000502:physiology*; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D002540:Cerebral Cortex / Q000378:metabolism; D004032:Diet; D015525:Fatty Acids, Omega-3 / Q000032:analysis; D006624:Hippocampus / Q000201:enzymology*; D007527:Isoenzymes / Q000378:metabolism*; D008055:Lipids / Q000737:chemistry*; D008569:Memory Disorders / Q000378:metabolism*; D051379:Mice; D008815:Mice, Inbred Strains; D023421:Models, Animal; D010713:Phosphatidylcholines / Q000008:administration & dosage; D010714:Phosphatidylethanolamines / Q000737:chemistry; D011493:Protein Kinase C / Q000378:metabolism*; D064546:Protein Kinase C beta; D014805:Vitamin B 12 / Q000008:administration & dosage
chemical_list: D000975:Antioxidants; D015525:Fatty Acids, Omega-3; D007527:Isoenzymes; D008055:Lipids; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D011493:Protein Kinase C; D064546:Protein Kinase C beta; D014805:Vitamin B 12