title: Identification of tumor metastasis suppressor region on the short arm of human chromosome 20.
abstract: Acquisition of metastatic ability by prostate cancer cells is the hallmark of their lethal trait and outcome. However, the genetic alterations underlying the clinical progression and pathogenesis of prostate cancer are not well understood. Several studies involving loss of heterozygosity (LOH) and comparative genomic hybridization analysis have identified distinctively altered regions on various human chromosomes, and genomic imbalance of chromosome 20 was implicated in progression and recurrence of prostate tumors. To examine the role of chromosome 20 in prostate neoplasms, we introduced this chromosome into highly metastatic rat prostate cancer cells using the microcell-mediated chromosome transfer technique. Introduction of the chromosome resulted in significant suppression of the metastatic ability of the hybrid cells, by as much as 98%, without any interference with the in vivo growth rate or tumorigenicity of primary tumor in SCID mice. Our STS-PCR analysis on 10 hybrid clones indicates that the suppressor activity of chromosome 20 is located in the p11.23-12 region. Further examination of the hybrid clones by experimental metastasis assay and histologic analysis as well as Matrigel invasion assay suggests the involvement of the suppressor region at an early stage of invasion and extravasation. We also investigated the status of the chromosome 20 suppressor region in pathology specimens from human prostate cancer patients and detected the frequent loss of this region in high-grade tumors. These results suggest the presence of a putative suppressor gene on human chromosome 20 that is functionally involved in development of prostate cancer metastases.
authors.affiliation: Department of Medical Microbiology and Immunology, Southern Illinois University School of Medicine, Springfield, Illinois 62702, USA.
authors.forename: G
authors.initials: G
authors.lastname: Goodarzi
authors.forename: T
authors.initials: T
authors.lastname: Mashimo
authors.forename: M
authors.initials: M
authors.lastname: Watabe
authors.forename: A P
authors.initials: AP
authors.lastname: Cuthbert
authors.forename: R F
authors.initials: RF
authors.lastname: Newbold
authors.forename: S K
authors.initials: SK
authors.lastname: Pai
authors.forename: S
authors.initials: S
authors.lastname: Hirota
authors.forename: S
authors.initials: S
authors.lastname: Hosobe
authors.forename: K
authors.initials: K
authors.lastname: Miura
authors.forename: S
authors.initials: S
authors.lastname: Bandyopadhyay
authors.forename: S C
authors.initials: SC
authors.lastname: Gross
authors.forename: K C
authors.initials: KC
authors.lastname: Balaji
authors.forename: K
authors.initials: K
authors.lastname: Watabe
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D002874:Chromosome Mapping; D002890:Chromosomes, Human, Pair 20 / Q000235:genetics*; D020869:Gene Expression Profiling; D016147:Genes, Tumor Suppressor / Q000235:genetics*; D006801:Humans; D006822:Hybrid Cells / Q000473:pathology; D008175:Lung Neoplasms / Q000473:pathology / Q000556:secondary; D008297:Male; D051379:Mice; D016513:Mice, SCID; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis / Q000235:genetics* / Q000473:pathology; D009368:Neoplasm Transplantation; D016133:Polymerase Chain Reaction; D011471:Prostatic Neoplasms / Q000235:genetics / Q000473:pathology; D051381:Rats; D016324:Sequence Tagged Sites; D014407:Tumor Cells, Cultured