title: [Determination of sucralose in foods by anion-exchange chromatography and reverse-phase chromatography].
abstract: Simple and rapid methods for the determination of sucralose in foods were developed using anion-exchange chromatography (AEC) with pulsed amperometric detection and reverse-phase HPLC with refractive index detection. Sucralose was extracted with water or methanol, and the extracted solution was cleaned up on a Sep-Pak C18 cartridge and a Sep-Pak Alumina N cartridge. The AEC separation was performed on a CarboPac PA1 column (4.0 mm i.d x 250 mm) using 100 mmol/L sodium hydroxide-50 mmol/L sodium acetate solution as the mobile phase at a flow rate of 1.0 mL/min. The recoveries of sucralose from foods were 80.6-102.0%, and quantitation limits from foods except chewing gum were 0.5 microgram/g (2 micrograms/g from chewing gum). The reverse-phase HPLC separation was performed on an Inertsil ODS-3V column (4.6 mm i.d x 150 mm) using methanol-water (25:75) as the mobile phase at a flow rate of 1.0 mL/min. The recoveries of sucralose from foods were 80.2-121.2%, and quantitation limits from foods except chewing gum were 5 micrograms/g (20 micrograms/g from chewing gum).
authors.affiliation: Kanagawa Prefectural Public Health Laboratory: 1-1-1, Nakao, Asahi-ku, Yokohama 241-0815, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Kishi
authors.forename: K
authors.initials: K
authors.lastname: Kawana
mesh_terms: D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D005504:Food Analysis / Q000379:methods*; D013395:Sucrose / Q000031:analogs & derivatives / Q000032:analysis*
chemical_list: D013395:Sucrose; C026285:trichlorosucrose