[Clinical and experimental study of effect on acute phase protein level of multiple organ dysfunction syndrome treated with dachengqi decoction].
OBJECTIVE
To investigate the level of acute phase protein level (APP) synthesized in hepatocytes in pathogenic process of multiple organ dysfunction syndrome (MODS) and the effect of Dachengqi Decoction (DCQD) on it.


METHODS
The levels of C-reactive protein (CRP), alpha 1-acid glycoprotein (alpha 1-AGP), alpha 1-antitrypsin total (alpha 1-AT), alpha 2-macroglobulin (alpha 2-MG), ceruloplasmine (Cp), hepatoglobin (Hp) and transferrin (Tf) synthesis were determined in 32 cases of MODS patients and rat models of MODS induced by acute infective peritonitis and mesenterial artery ischemia-reperfusion.


RESULTS
The serum APP level in MODS patients was increased and higher than that in the control group (P < 0.01). After 3 days of DCQD treatment, it reduced significantly (P < 0.05). Serum APP level in MODS model was also increased significantly, and after 3 days of DCQD treatment, it reduced significantly (P < 0.05). The APP secreting level of rat hepatocytes cultured in vitro raised after stimulated and activated by lipopolysacchride, which was reduced after cultured with rabbits' serum containing different concentration of effective ingredients of DCQD. The APP inhibiting effect of DCQD was dose dependent.


CONCLUSIONS
Purgation therapy with DCQD could reduce the stimulation of pathogenic factors on hepatocytes, protect the tissues and organs from injury caused by excessive inflammatory reaction.
Zhao
Q
Q
Dept. of Biochemistry and Molecular Biology, Tianjin Institute of Acute Abdominal Diseases, Tianjin 300100.
Cui
N
N
Li
J
J
D000209:Acute-Phase Proteins / Q000378:metabolism*; D000328:Adult; D000368:Aged; D000818:Animals; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D005260:Female; D022781:Hepatocytes / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D009102:Multiple Organ Failure / Q000188:drug therapy* / Q000209:etiology; D010538:Peritonitis / Q000150:complications; D051381:Rats; D017208:Rats, Wistar
D000209:Acute-Phase Proteins; D004365:Drugs, Chinese Herbal