title: Hematopoietic progenitors and stem cells in murine muscle.
abstract: We investigated hematopoietic capabilities of murine skeletal muscle using methylcellulose culture and transplantation into lethally irradiated mice. Muscle mononuclear cells (MNC) contained colony-forming cells and long-term engrafting cells. Studies using chimeric mice indicated a bone marrow origin of the hematopoietic cells in the muscle. We then separated muscle MNC by FACS sorting into Ly-5-positive cells and Ly-5-negative cells and analyzed their hematopoietic capability in vitro and in vivo. The hematopoietic progenitors and stem cells were present only in the Ly-5-positive fraction.
authors.affiliation: Department of Veterans Affairs Medical Center, 109 Bee Street, Medical University of South Carolina, Charleston, SC 29401, USA.
authors.forename: H
authors.initials: H
authors.lastname: Kawada
authors.forename: M
authors.initials: M
authors.lastname: Ogawa
mesh_terms: D000818:Animals; D000950:Antigens, Ly / Q000032:analysis; D001854:Bone Marrow Cells / Q000166:cytology; D018929:Cell Culture Techniques; D002678:Chimera / Q000502:physiology; D005434:Flow Cytometry; D006085:Graft Survival; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D023421:Models, Animal; D018482:Muscle, Skeletal / Q000166:cytology*; D018183:Transplantation Chimera
chemical_list: D000950:Antigens, Ly