Immunohistochemical study on the distribution of voltage-gated K(+) channels in rat brain following transient focal ischemia.
The present study examined the spatial localizations of voltage-gated K(+) (Kv) channels in the rat brain following transient focal ischemia, using immunohistochemistry. Increased expression of Kv1.2 was obvious in the cerebral cortex, dentate gyrus, amygdala, and hypothalamic areas at 3 days following ischemic insults. There was a significant increase in Kv1.2 immunoreactivity in several cortical regions, including cingulate cortex, infralimbic cortex, dorsal peduncular cortex and piriform cortex. On the contrary, Kv1.2 immunoreactivity had not significantly increased in the hippocampal CA1-3 regions although moderate Kv1.2 immunoreactivity was found in the cell bodies and processes of some neurons. Potentially the first demonstration of spatial changes in Kv1.2 channel expression could provide important molecular basis for altered neuronal excitability after ischemic brain injury.
Chung
Y H
YH
Department of Anatomy, Seoul National University College of Medicine, 28 Yongon-Dong, Chongno-Gu, 110-799, Seoul, South Korea. yoonhee2@snu.ac.kr
Kim
H S
HS
Shin
C M
CM
Kim
M J
MJ
Cha
C I
CI
D000679:Amygdala / Q000737:chemistry; D000818:Animals; D001923:Brain Chemistry; D002540:Cerebral Cortex / Q000737:chemistry; D018891:Dentate Gyrus / Q000737:chemistry; D007032:Hypothalamus, Anterior / Q000737:chemistry; D007150:Immunohistochemistry; D015640:Ion Channel Gating; D002546:Ischemic Attack, Transient / Q000378:metabolism*; D051663:Kv1.2 Potassium Channel; D008564:Membrane Potentials; D010286:Paraventricular Hypothalamic Nucleus / Q000737:chemistry; D015221:Potassium Channels / Q000032:analysis*; D024642:Potassium Channels, Voltage-Gated; D051381:Rats; D017207:Rats, Sprague-Dawley; D012686:Septal Nuclei / Q000737:chemistry
C496972:Kcna2 protein, rat; D051663:Kv1.2 Potassium Channel; D015221:Potassium Channels; D024642:Potassium Channels, Voltage-Gated