title: EMF1, a novel protein involved in the control of shoot architecture and flowering in Arabidopsis.
abstract: Shoot architecture and flowering time in angiosperms depend on the balanced expression of a large number of flowering time and flower meristem identity genes. Loss-of-function mutations in the Arabidopsis EMBRYONIC FLOWER (EMF) genes cause Arabidopsis to eliminate rosette shoot growth and transform the apical meristem from indeterminate to determinate growth by producing a single terminal flower on all nodes. We have identified the EMF1 gene by positional cloning. The deduced polypeptide has no homology with any protein of known function except a putative protein in the rice genome with which EMF1 shares common motifs that include nuclear localization signals, P-loop, and LXXLL elements. Alteration of EMF1 expression in transgenic plants caused progressive changes in flowering time, shoot determinacy, and inflorescence architecture. EMF1 and its related sequence may belong to a new class of proteins that function as transcriptional regulators of phase transition during shoot development.
authors.affiliation: Department of Plant and Microbial Biology, University of California, Berkeley, California 94720, USA.
authors.forename: D
authors.initials: D
authors.lastname: Aubert
authors.forename: L
authors.initials: L
authors.lastname: Chen
authors.forename: Y H
authors.initials: YH
authors.lastname: Moon
authors.forename: D
authors.initials: D
authors.lastname: Martin
authors.forename: L A
authors.initials: LA
authors.lastname: Castle
authors.forename: C H
authors.initials: CH
authors.lastname: Yang
authors.forename: Z R
authors.initials: ZR
authors.lastname: Sung
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development*; D029681:Arabidopsis Proteins; D003001:Cloning, Molecular; D018076:DNA, Complementary; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D018520:Plant Shoots / Q000254:growth & development*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000254:growth & development; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic / Q000235:genetics
chemical_list: D029681:Arabidopsis Proteins; D018076:DNA, Complementary; C435921:EMF1 protein, Arabidopsis; D010940:Plant Proteins