Investigation of microcontact transfer of proteins from a selectively plasma treated elastomer stamp by fluorescence microscopy and force microscopy.
Selective plasma treatment of the recessed regions of the elastomer stamps is shown to alter the resultant protein patterns. Fluorescence microscopy is demonstrated to be an excellent tool to discriminate between regions of microcontact printed fluorescent dye-labelled albumin in polystyrene. Atomic force microscopy and shear force microscopy are used to provide high-resolution images of the patterned protein layers. The formation and characteristics of the patterns formed by these alternative strategies is discussed.
Feng
X
X
Department of Pharmaceutical Sciences, The University of Nottingham, UK.
Roberts
C J
CJ
Armitage
D A
DA
Davies
M C
MC
Tendler
S J
SJ
Allen
S
S
Williams
P M
PM
D000818:Animals; D001798:Blood Proteins / Q000032:analysis*; D002417:Cattle; D018625:Microscopy, Atomic Force; D008856:Microscopy, Fluorescence; D012710:Serum Albumin, Bovine / Q000032:analysis
D001798:Blood Proteins; D012710:Serum Albumin, Bovine