Two telomerase reverse transcriptases (TERTs) expressed in Candida albicans.
The human pathogenic yeast Candida albicans contains two telomerase reverse transcriptase (TERT) genes. CaTERT1 and CaTERT2 appear either to be two alleles of the same gene or two entirely different genes that encode 867-residue proteins that differ by five amino acids. Both TERTs have a calculated pI of 9.5 and a M(r) of 100.9 kDa and are the smallest TERTs identified to date. Both genes appear to be expressed. Based on sequence similarity between CaTERT1 and the Saccharomyces cerevisiae orthologue Est2p, we suggest a revised alignment for motif E of Est2p. The identification of these TERT genes provides the first opportunity to study telomerase in an important human pathogen.
Metz
A M
AM
Department of Plant Sciences, Montana State University, Bozeman, MT 59717, USA.
Love
R A
RA
Strobel
G A
GA
Long
D M
DM
D000483:Alleles; D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry; D015139:Blotting, Southern; D002176:Candida albicans / Q000201:enzymology* / Q000235:genetics*; D004268:DNA-Binding Proteins; D020224:Expressed Sequence Tags; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008957:Models, Genetic; D008969:Molecular Sequence Data; D010802:Phylogeny; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D019098:Telomerase / Q000096:biosynthesis* / Q000235:genetics*
D000596:Amino Acids; D004268:DNA-Binding Proteins; D012333:RNA, Messenger; C509186:TERT protein, human; D019098:Telomerase