Sequence polymorphisms, allelic expression status and chromosome locations of the chicken IGF2 and MPR1 genes.
By screening 26 chicken breeds and lines, DNA polymorphisms were identified in the IGF2 and MPR1 genes, of which mammalian homologues are parentally imprinted, and the GAPD gene, a housekeeping control. Using the polymorphisms as genetic markers, we found that all three genes are expressed biallelically in embryonic tissues. IGF2 and MPR1 were mapped on chicken chromosomes 5 and 3, respectively, by fluorescence in situ hybridization, demonstrating conserved linkage homology between mammals and birds.
Yokomine
T
T
Division of Human Genetics, National Institute of Genetics, Graduate University for Advanced Studies, 1-111 Yata, Mishima 411-8540, Japan.
Kuroiwa
A
A
Tanaka
K
K
Tsudzuki
M
M
Matsuda
Y
Y
Sasaki
H
H
D000483:Alleles; D000818:Animals; D001483:Base Sequence; D001947:Breeding; D018797:Cell Cycle Proteins / Q000235:genetics*; D002642:Chick Embryo; D002645:Chickens / Q000235:genetics*; D017124:Conserved Sequence / Q000235:genetics; D010450:Endopeptidases; D005260:Female; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D018392:Genomic Imprinting / Q000235:genetics; D017404:In Situ Hybridization, Fluorescence; D007335:Insulin-Like Growth Factor II / Q000235:genetics*; D008297:Male; D020161:Physical Chromosome Mapping; D011110:Polymorphism, Genetic / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics*
D018797:Cell Cycle Proteins; D012333:RNA, Messenger; C115451:RPN11 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D007335:Insulin-Like Growth Factor II; D010450:Endopeptidases