Cell biology and matrix biochemistry of chondromyxoid fibroma.
We studied matrix composition and gene expression pattern in chondromyxoid fibromas on the protein and the messenger RNA levels. We could clearly identify focal chondrocytic differentiation within chondromyxoid fibroma by the expression and deposition of type II collagen, which is a marker of chondrocytic cell differentiation. We also were able to show expression of collagen types I, III, and VI in the neoplasm. The major tumor portion was, however, characterized by the presence of hydrated proteoglycans and only minor amounts of collagens, a matrix composition responsible for the myxoid matrix appearance of most parts of these neoplasms. By analyzing cytoprotein expression, we found S-100 protein restricted to cells of the chondroid areas, suggesting S-100 protein staining to be of little help as a positive diagnostic marker for chondromyxoid fibroma. Our data show a specific matrix composition of chondromyxoid fibroma, not previously found in other mesenchymal neoplasms, including chondroblastoma, osteochondroma, enchondroma, and chondrosarcoma. This justifies chondromyxoid fibroma as a specific neoplastic entity, both clinically and biologically.
Söder
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Department of Pathology, University of Erlangen-Nürnberg, Krankenhausstr. 8-10, D-91054 Erlangen, Germany.
Inwards
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Müller
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Kirchner
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Aigner
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D000293:Adolescent; D000328:Adult; D000368:Aged; D053674:Aggrecans; D014408:Biomarkers, Tumor / Q000032:analysis; D001859:Bone Neoplasms / Q000737:chemistry* / Q000473:pathology*; D002454:Cell Differentiation; D002455:Cell Division; D019902:Chondrocytes / Q000473:pathology*; D003094:Collagen / Q000032:analysis / Q000235:genetics; D016326:Extracellular Matrix Proteins; D005260:Female; D005350:Fibroma / Q000737:chemistry* / Q000473:pathology*; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D037181:Lectins, C-Type; D008297:Male; D008875:Middle Aged; D011509:Proteoglycans / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D009418:S100 Proteins / Q000032:analysis; D014746:Vimentin / Q000032:analysis
D053674:Aggrecans; D014408:Biomarkers, Tumor; D016326:Extracellular Matrix Proteins; D037181:Lectins, C-Type; D011509:Proteoglycans; D012333:RNA, Messenger; D009418:S100 Proteins; D014746:Vimentin; D003094:Collagen