title: Potential of Klebsiella pneumoniae cytotoxin toxoid as vaccine against klebsiellosis in rabbits and mice.
abstract: The protective efficacy of toxoids prepared from crude cytotoxin (polymyxin-B extract, PBE) and purified Klebsiella cytotoxins (KCTs) was studied in rabbits and mice. The toxoids of KCT-I and PBE were found to be protective against homologous as well as heterologous Klebsiella challenges, while toxoids of KCT-II and KCT-III afforded protection in mice against homologous Klebsiella infections. KCT-I and PBE toxoids also induced good humoral anti-Klebsiella response in rabbits with ELISA titres ranging from 20480 to 81480. Immunized female rabbits passed protective anti-Klebsiella immunoglobulins to their offsprings through colostra. Baby rabbits fed on colostrum of immunized rabbits withstood lethal Klebsiella infection up to 1 month of age, but not on the 50th day. Baby rabbits having an anti-Klebsiella IgG titre > or =1280 were fully protected against lethal dose of Klebsiella. The study revealed a significant protective efficacy of KCT-I and PBE toxoids against klebsiellosis in mice and rabbits.
authors.affiliation: Department of Veterinary Microbiology, College of Veterinary Sciences, GB Pant University of Agriculture and Technology, -263145, Pantnagar, India. singhbr@ivri.up.nic.in
authors.forename: B R
authors.initials: BR
authors.lastname: Singh
authors.forename: V D
authors.initials: VD
authors.lastname: Sharma
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D001428:Bacterial Vaccines / Q000627:therapeutic use*; D003603:Cytotoxins / Q000627:therapeutic use*; D005260:Female; D007115:Immunization Schedule; D007710:Klebsiella Infections / Q000517:prevention & control* / Q000662:veterinary*; D007711:Klebsiella pneumoniae / Q000276:immunology* / Q000472:pathogenicity; D051379:Mice; D011817:Rabbits; D014121:Toxoids / Q000627:therapeutic use*
chemical_list: D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D003603:Cytotoxins; D014121:Toxoids