title: Chiral separation of verapamil and some of its metabolites by HPLC and CE.
abstract: HPLC and CE assays were developed for chiral separations of verapamil and its metabolites in serum samples. Three chiral HPLC columns (Chiralcel OJ, Chiralpak AD and Chiralcel OD-R) were tested in normal and reverse-phase modes. All HPLC analyses were performed with fluorescence detection at 276 and 310 nm. CE was realized using CM-beta-CD as a chiral selector for the enantiomeric analysis. The results of HPLC and CE studies were compared and the possibilities for the applications in therapeutic drug monitoring were discussed.
authors.affiliation: Department of Analytical Chemistry, Slovak Technical University, Bratislava, Slovakia. branstet@cvt.suba.sk
authors.forename: E
authors.initials: E
authors.lastname: Brandsteterová
authors.forename: G
authors.initials: G
authors.lastname: Endresz
authors.forename: G
authors.initials: G
authors.lastname: Blaschke
mesh_terms: D000688:Amylose / Q000737:chemistry; D001711:Biotransformation; D002121:Calcium Channel Blockers / Q000737:chemistry / Q000302:isolation & purification* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid; D003505:Cyclodextrins; D019075:Electrophoresis, Capillary; D006801:Humans; D007202:Indicators and Reagents; D013237:Stereoisomerism; D014700:Verapamil / Q000031:analogs & derivatives* / Q000097:blood / Q000737:chemistry / Q000302:isolation & purification* / Q000493:pharmacokinetics
chemical_list: D002121:Calcium Channel Blockers; D003505:Cyclodextrins; D007202:Indicators and Reagents; D000688:Amylose; C016904:norverapamil; D014700:Verapamil