Azospirillum spp. metabolize [17,17-2H2]gibberellin A20 to [17,17-2H2]gibberellin A1 in vivo in dy rice mutant seedlings.
Azospirillum spp. are endophytic bacteria with beneficial effects on cereals--effects partially attributed to gibberellin production by the microorganisms. Azospirillum lipoferum and Azospirillum brasilense inoculated to rice dy mutant reversed dwarfism in seedlings incubated with [17,17-2H2]GA20 with formation of [17,17-2H2]GA1, showing the in vivo capacity to perform the 3beta-hydroxylation. When prohexadione-Ca, an inhibitor of late steps in gibberellin biosynthesis, was added to the culture medium, no complementation was observed and no [17,17-2H2]GA1 was produced. The latter suggests that the bacterial operating enzyme may be a 2-oxoglutarate-dependent dioxygenase, similar to those of plants.
Cassán
F D
FD
Laboratorio de Fisiología Vegetal, Departamento de Ciencias Naturales, Universidad Nacional de Río Cuarto, Campus Universitario, 5800 Río Cuarto, Argentina.
Lucangeli
C D
CD
Bottini
R
R
Piccoli
P N
PN
D016949:Azospirillum / Q000254:growth & development / Q000378:metabolism*; D015806:Azospirillum brasilense / Q000254:growth & development / Q000378:metabolism*; D003903:Deuterium; D008401:Gas Chromatography-Mass Spectrometry; D005875:Gibberellins / Q000737:chemistry / Q000378:metabolism*; D006868:Hydrolysis; D016296:Mutagenesis; D012275:Oryza / Q000235:genetics / Q000254:growth & development / Q000378:metabolism / Q000382:microbiology*; D018517:Plant Roots / Q000254:growth & development / Q000378:metabolism / Q000382:microbiology; D012988:Soil Microbiology
D005875:Gibberellins; C422660:gibberellin A1; D003903:Deuterium; C007842:gibberellic acid