Interleukin-1beta up-regulates expression of neurofilament light in human neuronal cells.
Elevated expression of interleukin-1 (IL-1beta), a pro-inflammatory cytokine secreted by activated microglia, is a pathogenic marker of numerous neurodegenerative processes including Alzheimer's disease (AD). We have characterized a link between IL-1beta and the 68-kDa neurofilament light (NF-L) protein, which is a major component of the neuronal cytoskeleton. Using human brain aggregate cultures, we found that IL-1beta treatment significantly increased NF-L expression in primary neurons. Analysis of mRNA levels demonstrated elevated NF-L expression within 72 h while imaging of neurons by immunofluorescent staining for NF-L confirmed IL-1beta-induced NF-L protein expression. These observations suggest a potential inflammatory-induced mechanism for deregulation of an important cytoskeletal protein, NF-L, possibly leading to neuronal dysfunction.
Rempel
H
H
Department of Laboratory Medicine, Veterans Affairs Medical Center, San Francisco, California 94121, USA.
Kusdra
L
L
Pulliam
L
L
D000544:Alzheimer Disease / Q000378:metabolism; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free; D005786:Gene Expression Regulation; D006801:Humans; D015151:Immunoblotting; D007375:Interleukin-1 / Q000494:pharmacology*; D008856:Microscopy, Fluorescence; D016900:Neurofilament Proteins / Q000235:genetics / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D016895:Culture Media, Serum-Free; D007375:Interleukin-1; D016900:Neurofilament Proteins; D014409:Tumor Necrosis Factor-alpha; C044882:neurofilament protein L