title: Evaluation of a new fourth-generation microwell enzyme-linked immunosorbent assay for detection of HIV-1 subtype B and E antibodies.
abstract: The recent fourth-generation enzyme-immunoassays have been used to increase the sensitivity for detecting HIV-1 antibodies and reduce the window period of HIV infection. The HIV antigens utilized in those assays were prepared from HIV-1 clade B which is different from HIV-1 subtypes circulating in Thailand. We evaluated 323 HIV-1 seropositives either B or E subtype to determine whether they were detected with the new combined anti-HIV and the p24 Ag assay. Under evaluation we found that this enzyme immunoassay manufactured by Organon Teknika showed the high sensitivity and specificity with a greater delta (delta) value with B than E subtypes samples (+15.29 vs +5.73).
authors.affiliation: Army Institute of Pathology, Bangkok, Thailand. penprapac@thai.amedd.army.mil
authors.forename: P
authors.initials: P
authors.lastname: Chanbancherd
authors.forename: N
authors.initials: N
authors.lastname: Limpairojn
authors.forename: M S
authors.initials: MS
authors.lastname: de Souza
authors.forename: A
authors.initials: A
authors.lastname: Jugsudee
authors.forename: P
authors.initials: P
authors.lastname: Julananto
authors.forename: P
authors.initials: P
authors.lastname: Tienamporn
authors.forename: W
authors.initials: W
authors.lastname: Leucha
authors.forename: C
authors.initials: C
authors.lastname: Tasaniyananda
authors.forename: A E
authors.initials: AE
authors.lastname: Brown
mesh_terms: D000595:Amino Acid Sequence; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000592:standards*; D015483:HIV Antibodies / Q000097:blood*; D015699:HIV Envelope Protein gp120 / Q000737:chemistry; D015497:HIV-1 / Q000145:classification / Q000276:immunology*; D006801:Humans; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry; D012680:Sensitivity and Specificity; D013045:Species Specificity
chemical_list: D015483:HIV Antibodies; D015699:HIV Envelope Protein gp120; C059762:HIV envelope protein gp120 (305-321); D010446:Peptide Fragments