Reduced protein adsorption and platelet adhesion by controlled variation of oligomaltose surfactant polymer coatings.
A series of oligomaltose surfactant polymers were prepared by the simultaneous coupling of hydrophilic maltolactone [of 2(M2), 7(M7), or 15(M15) glucose units] and hydrophobic N-(hexanoyloxy)succinimide (Hex) groups to the amino groups of a poly(vinyl amine) backbone. The surfactants were characterized by FTIR and 1H-NMR spectroscopies for purity and composition. Contact-angle and AFM measurements confirmed full monolayer adsorption for all surfactants on a model surface, highly oriented pyrolitic graphite, while full coverage was observed on polyethylene only for PVAm (M7:Hex) due to the optimal M7:Hex ratio and Hex chain density. On graphite, protein resistance increased with increasing coating thickness from 81.4 to 85.8 to 95.8% for the M2, M7, and M15 surfactants, respectively. Additionally, static platelet adhesion on all three surfactants dropped substantially to 15% (M2), 17% (M7), and 16% (M15)compared to glass (adhesion normalized to 100%) and a polyurethane (24%) surface. Protein- and platelet-resistant properties of the controlled oligomaltose layers are discussed by analysis of molecular modeling, oligomaltose and hexanoyl chain densities, and surfactant stability.
Ruegsegger
M A
MA
Department of Biomedical Engineering, Wickenden Building 202, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106-7207, USA.
Marchant
R E
RE
D000327:Adsorption / Q000187:drug effects; D001363:Awards and Prizes; D001672:Biocompatible Materials / Q000378:metabolism; D002240:Carbohydrate Sequence; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D006801:Humans; D066298:In Vitro Techniques; D009682:Magnetic Resonance Spectroscopy; D008320:Maltose / Q000031:analogs & derivatives* / Q000138:chemical synthesis; D018625:Microscopy, Atomic Force; D008958:Models, Molecular; D008970:Molecular Weight; D009844:Oligosaccharides / Q000737:chemistry*; D015539:Platelet Activation / Q000187:drug effects*; D010973:Platelet Adhesiveness / Q000187:drug effects*; D010980:Platelet Membrane Glycoproteins / Q000737:chemistry* / Q000187:drug effects*; D011095:Polyethylenes / Q000378:metabolism; D011108:Polymers / Q000737:chemistry; D011140:Polyurethanes / Q000378:metabolism*; D011145:Polyvinyls / Q000378:metabolism*; D013499:Surface Properties; D013501:Surface-Active Agents / Q000138:chemical synthesis* / Q000737:chemistry
D001672:Biocompatible Materials; D020099:Coated Materials, Biocompatible; D009844:Oligosaccharides; D010980:Platelet Membrane Glycoproteins; D011095:Polyethylenes; D011108:Polymers; D011140:Polyurethanes; D011145:Polyvinyls; D013501:Surface-Active Agents; C008996:poly(vinylamine); D008320:Maltose