title: Identification of two down-regulated genes in rat liver allografts by mRNA differential display.
abstract: Total RNA differential display (DD) using random primers was performed for rat orthotopic liver transplantation (OLT) models. DA (RT1a) donor livers were transplanted into DA, PVG (RT1c), and LEW (RT1l) recipients: (1) syngeneic OLT (DA-DA): no rejection occurs; (2) allogeneic OLT (DA-PVG): rejection occurs, but is naturally overcome without immunosuppression; (3) allogeneic OLT (DA-LEW): animals die of acute rejection within 14 days. cDNA was isolated from selected bands, re-amplified for sequencing, and confirmed by Northern blots. Two down-regulated genes were observed in day-7 allogeneic OLT livers (DA-PVG, DA-LEW), while they were consistently expressed in day-7 syngeneic OLT (DA-DA) livers. These two genes were identified as alpha-glutathione sulfotransferase (alpha-GST) Ya gene and estrogen sulfotransferase (EST), respectively. Northern blots confirmed that their expression was down-regulated in OLT (DA-PVG) livers on days 7-26 and gradually restored. The mRNA expression of GST and EST may be good markers to predict rejection or induction of tolerance.
authors.affiliation: Department of Surgery, Chang Gung Memorial Hospital, Niao-Sung, Kaohsiung, Taiwan.
authors.forename: Y C
authors.initials: YC
authors.lastname: Lin
authors.forename: S
authors.initials: S
authors.lastname: Goto
authors.forename: T L
authors.initials: TL
authors.lastname: Pan
authors.forename: Y R
authors.initials: YR
authors.lastname: Hong
authors.forename: C L
authors.initials: CL
authors.lastname: Lin
authors.forename: R
authors.initials: R
authors.lastname: Lord
authors.forename: K C
authors.initials: KC
authors.lastname: Chiang
authors.forename: C Y
authors.initials: CY
authors.lastname: Lai
authors.forename: H P
authors.initials: HP
authors.lastname: Tseng
authors.forename: L W
authors.initials: LW
authors.lastname: Hsu
authors.forename: S
authors.initials: S
authors.lastname: Iwashita
authors.forename: S
authors.initials: S
authors.lastname: Kitano
authors.forename: C L
authors.initials: CL
authors.lastname: Chen
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D015536:Down-Regulation; D020869:Gene Expression Profiling; D008099:Liver / Q000503:physiopathology*; D016031:Liver Transplantation; D008297:Male; D051381:Rats; D011917:Rats, Inbred Lew; D011919:Rats, Inbred Strains; D017385:Sequence Homology; D015238:Sulfotransferases / Q000235:genetics*; D013997:Time Factors
chemical_list: D015238:Sulfotransferases; C020122:estrone sulfotransferase