title: Structure and ligand recognition of the PB1 domain: a novel protein module binding to the PC motif.
abstract: PB1 domains are novel protein modules capable of binding to target proteins that contain PC motifs. We report here the NMR structure and ligand-binding site of the PB1 domain of the cell polarity establishment protein, Bem1p. In addition, we identify the topology of the PC motif-containing region of Cdc24p by NMR, another cell polarity establishment protein that interacts with Bem1p. The PC motif-containing region is a structural domain offering a scaffold to the PC motif. The chemical shift perturbation experiment and the mutagenesis study show that the PC motif is a major structural element that binds to the PB1 domain. A structural database search reveals close similarity between the Bem1p PB1 domain and the c-Raf1 Ras-binding domain. However, these domains are functionally distinct from each other.
authors.affiliation: Tokyo Metropolitan Institute of Medical Science, 3-18-22 Honkomagome, Bunkyo-ku, Tokyo 113-8613, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Terasawa
authors.forename: Y
authors.initials: Y
authors.lastname: Noda
authors.forename: T
authors.initials: T
authors.lastname: Ito
authors.forename: H
authors.initials: H
authors.lastname: Hatanaka
authors.forename: S
authors.initials: S
authors.lastname: Ichikawa
authors.forename: K
authors.initials: K
authors.lastname: Ogura
authors.forename: H
authors.initials: H
authors.lastname: Sumimoto
authors.forename: F
authors.initials: F
authors.lastname: Inagaki
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D018797:Cell Cycle Proteins / Q000737:chemistry* / Q000235:genetics; D005656:Fungal Proteins / Q000737:chemistry* / Q000235:genetics; D020662:Guanine Nucleotide Exchange Factors; D006801:Humans; D008024:Ligands; D051379:Mice; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000737:chemistry; D011493:Protein Kinase C / Q000737:chemistry; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011518:Proto-Oncogene Proteins / Q000737:chemistry* / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins; D029702:Schizosaccharomyces pombe Proteins; D055672:Static Electricity; D000099221:Protein Kinase C zeta
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D018797:Cell Cycle Proteins; D005656:Fungal Proteins; D020662:Guanine Nucleotide Exchange Factors; D008024:Ligands; D010750:Phosphoproteins; D011493:Protein Kinase C; D011518:Proto-Oncogene Proteins; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; D029702:Schizosaccharomyces pombe Proteins; D000099221:Protein Kinase C zeta; C073594:CDC24 protein, S cerevisiae; C079789:neutrophil cytosol factor 67K; C089770:scd2 protein, S pombe; C073129:BEM1 protein, S cerevisiae