Immunohistochemical localization of calbindin D-28k in the migratory pathway from the rat olfactory placode.
The spatiotemporal localization of calbindin D-28k (Calb), a calcium-binding protein, was examined immunohistochemically in the developing rat olfactory system with special reference to cell migration from the olfactory placode. Calb immunoreactivity was first detected at embryonic day 12 (E12) in a few cells just outside the olfactory epithelium, and at E13, Calb-immunoreactive cells were found scattered in the laminin-rich mesenchyme. By E14, Calb-immunoreactive cells had increased in number and were seen along the entire migratory route between the vomeronasal organ, a derivative of the medial olfactory pit, and the ventromedial surface of the telencephalic vesicle. Calb neurones were not seen in the olfactory epithelium, a derivative of the lateral olfactory pit. Although the distribution pattern of Calb-immunoreactive cells was similar to that of luteinizing hormone releasing hormone (LHRH)-producing neurones, which are known to originate in the vomeronasal organ and migrate into the forebrain, Calb and LHRH immunoreactivities were contained in separate neuronal populations. Calb-immunoreactive cells were localized along the vomeronasal nerves, identified by labelling the vomeronasal organ with the lipophilic dye, DiI, and strongly immunoreactive for neural cell adhesion molecule (NCAM). These data strongly suggest that, in addition to LHRH neurones, the rat vomeronasal organ generates Calb-immunoreactive neurones which migrate along the vomeronasal nerves to enter the forebrain. The final fate and functional importance of these cells remains to be determined.
Toba
Y
Y
Department of Developmental Morphology, Tokyo Metropolitan Institute for Neuroscience, Fuchu, Japan.
Ajiki
K
K
Horie
M
M
Sango
K
K
Kawano
H
H
D000818:Animals; D064026:Calbindins; D002232:Carbocyanines; D002465:Cell Movement / Q000502:physiology*; D005456:Fluorescent Dyes; D007987:Gonadotropin-Releasing Hormone / Q000032:analysis; D007150:Immunohistochemistry; D007797:Laminin / Q000032:analysis; D019006:Neural Cell Adhesion Molecules / Q000032:analysis; D009831:Olfactory Mucosa / Q000737:chemistry* / Q000166:cytology / Q000196:embryology*; D009833:Olfactory Pathways / Q000737:chemistry / Q000166:cytology / Q000196:embryology; D051381:Rats; D017207:Rats, Sprague-Dawley; D064030:S100 Calcium Binding Protein G / Q000032:analysis*; D019147:Vomeronasal Organ / Q000737:chemistry / Q000166:cytology / Q000196:embryology
C035005:3,3'-dihexadecylindocarbocyanine; D064026:Calbindins; D002232:Carbocyanines; D005456:Fluorescent Dyes; D007797:Laminin; D019006:Neural Cell Adhesion Molecules; D064030:S100 Calcium Binding Protein G; D007987:Gonadotropin-Releasing Hormone