CD8- dendritic cell activation status plays an integral role in influencing Th2 response development.
Whether dendritic cells (DC) play a passive or active role in Th2 response induction is poorly understood. In this study, we show that CD8- DC pulsed with Th2-polarizing Ag (soluble egg Ag (SEA)) from Schistosoma mansoni potently stimulate Th2 responses in vivo and in vitro while failing to undergo a conventional maturation process. Thus, in contrast to DC pulsed with the Th1 response inducing Ag Propionebacterium acnes, SEA-exposed DC exhibit a phenotype that is most similar to that of immature DC, failing to up-regulate expression of CD40, CD54, CD80, CD86, or OX40L; producing no detectable IL-4, IL-10, or IL-12; and displaying only a minor increase in MHC class II expression. Importantly, in vitro derived DC exposed to SEA were phenotypically similar to CD8- DC isolated from active S. mansoni infection. By discriminating between different types of pathogen and responding appropriately, CD8- DC play a major role in the decision process to mount either a Th1 or Th2 response.
MacDonald
A S
AS
Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA.
Straw
A D
AD
Bauman
B
B
Pearce
E J
EJ
D000818:Animals; D000942:Antigens, Bacterial / Q000494:pharmacology; D000947:Antigens, Helminth / Q000494:pharmacology; D019013:CD40 Antigens / Q000502:physiology; D016827:CD8 Antigens / Q000096:biosynthesis; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology / Q000469:parasitology; D005260:Female; D016130:Immunophenotyping; D018664:Interleukin-12 / Q000096:biosynthesis; D051379:Mice; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D011425:Propionibacterium acnes / Q000276:immunology; D012550:Schistosoma mansoni / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology*
D000942:Antigens, Bacterial; D000947:Antigens, Helminth; D019013:CD40 Antigens; D016827:CD8 Antigens; D018664:Interleukin-12