title: Removal of HA and TPS implant coatings and fibroblast attachment on exposed surfaces.
abstract: The removal of implant coatings may be necessary if rough implant surfaces are exposed subgingivally due to progressive peri-implant bone loss or if they are also supragingivally exposed because of progressive gingival recession, thus facilitating plaque formation and impairing tooth cleaning done at home. The aim of this experimental study was to develop diamond-coated files and rubberized polishers for machine-driven instrumentation of implant cylinders, and present an instrumentation concept for the complete removal of rough titanium plasma spray (TPS) and hydroxylapatite (HA) layers and polishing exposed titanium surfaces. The surface structure and its possible contamination by the instrumentation process was investigated using laser profilometry, scanning electron microscopy, and x-ray fluorescence analysis. The effect of impurities on the growth of human fibroblasts was tested in cell culture. The results show that TPS and HA implant coatings can be removed with the modified implant files. After polishing the exposed titanium surfaces, roughness depths of Rz=4.6 microm (TPS) and Rz=5.7 microm (HA) and, for implant cylinders, 3.4 microm were found. During the instrumentation of implant surfaces, contamination with the material of the instrument must be expected. It was shown that, in principle, growth of human gingival fibroblasts on the instrumented surfaces is possible. The cells were intimately associated with one another and, compared to culture controls, demonstrated good adhesion with strict orientation to the microstructure of the scoring left by instrumentation. The biological consequences and mechanisms of cell adhesion on instrumented surfaces require further investigation.
authors.affiliation: Department of Periodontics, School of Dentistry, University of Kiel, Arnold-Heller-Str. 16, 24105 Kiel 1, Germany. ruehling@konspar.uni-kiel.de
authors.forename: A
authors.initials: A
authors.lastname: Rühling
authors.forename: A
authors.initials: A
authors.lastname: Hellweg
authors.forename: T
authors.initials: T
authors.lastname: Kocher
authors.forename: H C
authors.initials: HC
authors.lastname: Plagmann
mesh_terms: D002448:Cell Adhesion; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible; D003754:Dental High-Speed Equipment; D015921:Dental Implants; D003762:Dental Instruments; D003775:Dental Polishing / Q000295:instrumentation*; D017886:Durapatite; D005347:Fibroblasts / Q000502:physiology; D006801:Humans; D007834:Lasers; D008855:Microscopy, Electron, Scanning; D013499:Surface Properties; D014025:Titanium
chemical_list: D020099:Coated Materials, Biocompatible; D015921:Dental Implants; D017886:Durapatite; D014025:Titanium