Sensitive determination of pethidine in body fluids by surface ionization organic mass spectrometry.
We have presented a simple and sensitive method for determining pethidine, a narcotic analgesic drug in body fluids by gas chromatography (GC)/surface ionization organic mass spectrometry (SIOMS). Good linearity was obtained in the range of 0.625-25 ng/ml of whole blood and urine by mass chromatography, and in the range of 0.05-2 ng/ml of whole blood by selected ion monitoring (SIM). Pethidine and diphenylpyraline (internal standard) were extracted from body fluids with Bond Elut Certify cartridges; their recoveries were above 95%. The detection limits (signal-to-noise ratio=3) were estimated to be 0.2 ng/ml of whole blood or urine by mass chromatography, 0.02 ng/ml of whole blood by SIM.
Ishii
A
A
Department of Legal Medicine and Bioethics, Nagoya University Graduate School of Medicine, Japan. akishii@med.nagoya-u.ac.jp
Kurihara
R
R
Watanabe-Suzuki
K
K
Kumazawa
T
T
Seno
H
H
Matsushima
H
H
Suzuki
O
O
Katsumata
Y
Y
D000701:Analgesics, Opioid / Q000032:analysis*; D001826:Body Fluids / Q000737:chemistry*; D006801:Humans; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D008614:Meperidine / Q000032:analysis* / Q000097:blood / Q000652:urine; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000701:Analgesics, Opioid; D008614:Meperidine