Simultaneous determination of magnesium L-ascorbyl-2-phosphate and kojic acid in cosmetic bleaching products by using a microbore column and ion-pair liquid chromatography.
A rapid, sensitive, and reliable method was developed for the simultaneous determination of magnesium L-ascorbyl-2-phosphate (AS-PM) and kojic acid (KA), commonly using bleaching agents in cosmetic products. The method uses a prepacked C18 microbore column for ion-pair liquid chromatography with photodiode array detection. The eluant is 0.5mM tetrabutylammonium bromide and 0.05M phosphate buffer, pH 5, containing 5% methanol, at a flow rate of 70 microL/min. The determinations of AS-PM and KA were linear in the range of 0.5-1,000 and 0.25-500 ng, respectively. The precision of the assay ranged from 0.31 to 2.19%, and the detection limits (signal-to-noise ratio = 3) were 0.04 and 0.02 ng for AS-PM and KA, respectively.
Shih
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Hung-Kuang Institute of Technology, Department of Applied Cosmetology, Taichung, Taiwan.
D001205:Ascorbic Acid / Q000031:analogs & derivatives* / Q000032:analysis*; D002853:Chromatography, Liquid; D003358:Cosmetics / Q000032:analysis*; D011753:Pyrones / Q000032:analysis*
D003358:Cosmetics; D011753:Pyrones; C011669:ascorbate-2-phosphate; C011890:kojic acid; D001205:Ascorbic Acid