Comparison of VIDAS enzyme-linked fluorescent immunoassay using Moore swab sampling and conventional culture method for Salmonella detection in bulk tank milk and in-line milk filters in California dairies.
A comparison of the VIDAS Salmonella (SLM) assay using the manufacturer's recommended sampling method or a Moore swab sampling method demonstrated that the Moore swab method detected a greater number of positive samples (83.0% vs. 67.92%). When results using a conventional culture technique were compared to the VIDAS SLM assay using Moore swab sampling, there was good agreement (97.5%). When the VIDAS SLM assay was used to test in-line milk filters and compared to results from the conventional culture method, there was also good correlation between test results (95.57%). Overall, the VIDAS SLM assay using a modified sampling method compared favorably to the conventional culture method and had the advantages of taking less time to obtain a negative or presumptive positive result, being less technically complicated and requiring less screening of non-lactose fermenting colonies from negative samples.
Walker
R L
RL
California Animal Health and Food Safety Laboratory, School of Veterinary Medicine, University of California, Davis 95616, USA. rlwalker@ucdavis.edu
Kinde
H
H
Anderson
R J
RJ
Brown
A E
AE
D000818:Animals; D001431:Bacteriological Techniques; D002417:Cattle; D015169:Colony Count, Microbial; D003612:Dairying / Q000295:instrumentation*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D005374:Filtration / Q000295:instrumentation; D015200:Fluoroimmunoassay / Q000379:methods; D005516:Food Microbiology; D008892:Milk / Q000382:microbiology*; D012475:Salmonella / Q000302:isolation & purification*