title: Measurement of DNA damage in mammalian cells exposed in vitro to radiofrequency fields at SARs of 3-5 W/kg.
abstract: In the present study, we determined whether exposure of mammalian cells to 3.2-5.1 W/kg specific absorption rate (SAR) radiofrequency fields could induce DNA damage in murine C3H 10T(1/2) fibroblasts. Cell cultures were exposed to 847.74 MHz code-division multiple access (CDMA) and 835.62 frequency-division multiple access (FDMA) modulated radiations in radial transmission line (RTL) irradiators in which the temperature was regulated to 37.0 +/- 0.3 degrees C. Using the alkaline comet assay to measure DNA damage, we found no statistically significant differences in either comet moment or comet length between sham-exposed cells and those exposed for 2, 4 or 24 h to CDMA or FDMA radiations in either exponentially growing or plateau-phase cells. Further, a 4-h incubation after the 2-h exposure resulted in no significant changes in comet moment or comet length. Our results show that exposure of cultured C3H 10T(1/2) cells at 37 degrees C CDMA or FDMA at SAR values of up to 5.1 W/kg did not induce measurable DNA damage.
authors.affiliation: Section of Cancer Biology, Radiation Oncology Center, Mallinckrodt Institute of Radiology, Washington University, St. Louis, Missouri 63108, USA.
authors.forename: L
authors.initials: L
authors.lastname: Li
authors.forename: K S
authors.initials: KS
authors.lastname: Bisht
authors.forename: I
authors.initials: I
authors.lastname: LaGroye
authors.forename: P
authors.initials: P
authors.lastname: Zhang
authors.forename: W L
authors.initials: WL
authors.lastname: Straube
authors.forename: E G
authors.initials: EG
authors.lastname: Moros
authors.forename: J L
authors.initials: JL
authors.lastname: Roti Roti
mesh_terms: D000818:Animals; D002460:Cell Line; D020552:Comet Assay; D004247:DNA / Q000528:radiation effects*; D004249:DNA Damage; D066298:In Vitro Techniques; D051379:Mice; D008809:Mice, Inbred C3H; D011846:Radio Waves
chemical_list: D004247:DNA