E2A and HEB activate the pre-TCR alpha promoter during immature T cell development.
The pre-TCRalpha (pTalpha) is exclusively expressed in immature thymocytes and constitutes the pre-TCR complex with TCRbeta, which regulates early T cell differentiation. Despite the recent identification of the pTalpha enhancer, the contribution of the promoter region, the direct DNA-protein interaction, and the regulation of such interaction along with T cell development have not been investigated. We analyzed the pTalpha promoter region and identified the critical elements for transcription of the pTalpha gene. The pTalpha promoter was found to contain two consecutive E-box elements that are critical for pTalpha transcription. The E-box elements in the promoter region formed the specific DNA-protein complex that was exclusively observed in immature thymocytes, not in mature thymocytes and T cells. The E proteins in this complex were identified as E2A and HeLa E-box binding protein (HEB), and overexpression of E2A and HEB resulted in activation of the pTalpha promoter. The binding complex in the consecutive E-boxes in the pTalpha promoter changed along with T cell development, as a distinct DNA-binding complex was observed in mature T cells. Comparing the E-box regions in the enhancer and the promoter, those in the promoter appear to make a greater contribution to pTalpha gene transcription.
Takeuchi
A
A
Department of Molecular Genetics, Chiba University Graduate School of Medicine, Chiba, Japan.
Yamasaki
S
S
Takase
K
K
Nakatsu
F
F
Arase
H
H
Onodera
M
M
Saito
T
T
D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins; D002454:Cell Differentiation / Q000235:genetics / Q000276:immunology; D002460:Cell Line; D004247:DNA / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism / Q000502:physiology*; D024721:E-Box Elements / Q000276:immunology; D005786:Gene Expression Regulation / Q000276:immunology*; D006801:Humans; D006825:Hybridomas; D046911:Macromolecular Substances; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D011401:Promoter Regions, Genetic / Q000276:immunology*; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics* / Q000378:metabolism; D012045:Regulatory Sequences, Nucleic Acid; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism / Q000502:physiology*; D014162:Transfection
D051792:Basic Helix-Loop-Helix Proteins; D004247:DNA; D004268:DNA-Binding Proteins; D046911:Macromolecular Substances; D008562:Membrane Glycoproteins; D016693:Receptors, Antigen, T-Cell, alpha-beta; D014157:Transcription Factors; C407909:TCF3 protein, human; C489532:Tcf12 protein, mouse; C090377:pre-T cell receptor alpha; C073366:TCF12 protein, human