[Research on the separation behavior of acidic drugs in capillary electrophoresis with reversed direction of electroosmotic flow].
The separation behavior of acidic drugs in capillary electrophoresis with reversal of electroosmotic flow was investigated systematically. Acetylsalicylic acid and its related compound salicylic acid were employed as objective drugs. The cationic surfactant cetyltrimethylammonium bromide (CTAB) was used as reversed reagent for electroosmotic flow. The experimental conditions, such as, cationic surfactant concentration, buffer pH and organic additives, which affected migration time, peak shape and column efficiency, were studied in detail. The experimental results indicated that high speed analysis could be achieved in the capillary electrophoresis with reversed electroosmotic flow induced by cationic surfactant when acidic drugs were analysed. The poor peak shape and low column efficiency caused by the interactions of CTAB with acidic anions would be improved by adding proper organic additive, such as, beta-cyclodextrin or acetonitrile.
Lin
M
M
Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing, 210009.
Feng
M
M
Zhang
Z
Z
An
D
D
Fan
G
G
D001241:Aspirin / Q000302:isolation & purification*; D000077286:Cetrimonium; D002593:Cetrimonium Compounds; D019075:Electrophoresis, Capillary / Q000379:methods*; D020156:Salicylic Acid / Q000302:isolation & purification*; D013501:Surface-Active Agents
D002593:Cetrimonium Compounds; D013501:Surface-Active Agents; D020156:Salicylic Acid; D001241:Aspirin; D000077286:Cetrimonium