title: Swelling-activated chloride currents in embryonic chick heart cells.
abstract: OBJECTIVE
  To characterize a swelling-activated chloride current, I(Cl, swell), in white Leghorn chick heart cells and the effects of chlorpromazine (CPZ) effects.
  
  
  METHODS
  The patch-clamp technique in the whole-cell configuration was used.
  
  
  RESULTS
  Hyposmotic swelling elicited I(Cl, swell) in white Leghorn chick heart cells. The current amplitude increased from (452 +/- 200) pA to (849 +/- 373) pA with a reduction of osmolarity from 300 mmol.L-1 to 270 mmol.L-1, 4',4-Diisothiocyanostilbene-2,2'-disulphonic acid (DIDS) 100 mumol.L-1 decreased I(Cl, swell) from (1196 +/- 505) pA to (830 +/- 328) pA in hyposmotic solution. In white Leghorn chick heart cells I(Cl, swell) was not induced by CPZ 30 mumol.L-1, which is different from the case of E coli spheroplast.
  
  
  CONCLUSIONS
  Swelling-activated chloride current was activated by hyposmotic swelling in white Leghorn chick heart cells. The mechanism for activating the current is different from that of mechanosensitive ion channels of E coli.
authors.affiliation: Department of Physiology and Biophysics, Liren Laboratory, Fudan University, Shanghai 200433, China.
authors.forename: H
authors.initials: H
authors.lastname: Wei
authors.forename: Y A
authors.initials: YA
authors.lastname: Mei
authors.forename: M M
authors.initials: MM
authors.lastname: Wu
authors.forename: J T
authors.initials: JT
authors.lastname: Sun
authors.forename: H Q
authors.initials: HQ
authors.lastname: Zhou
authors.forename: Z H
authors.initials: ZH
authors.lastname: Zhang
mesh_terms: D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid / Q000494:pharmacology; D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D018118:Chloride Channels / Q000187:drug effects*; D002746:Chlorpromazine / Q000494:pharmacology*; D006321:Heart / Q000502:physiology; D009206:Myocardium / Q000166:cytology*; D018408:Patch-Clamp Techniques
chemical_list: D018118:Chloride Channels; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; D002746:Chlorpromazine