Effects of the purified IGF-I complex on the capacitation and acrosome reaction of rabbit spermatozoa.
A protein complex containing IGF-I, purified from rabbit seminal plasma, was used to investigate its effects on the capacitation and acrosome reaction of rabbit spermatozoa. Uncapacitated sperm (Pattern F), capacitated sperm (Pattern B), and acrosome-reacted sperm (Pattern AR) were determined by CTC staining, and the results were validated by PSA-staining. The addition of the IGF-I complex to the capacitative medium directed the spermatozoa to spontaneous acrosome reaction. On the other hand, IGF-I complex, added to capacitated spermatozoa, acted as inducer of the acrosome reaction. Results of IVF experiments showed high rates of fertilization with capacitated spermatozoa, acrosome-reacted by either A23187 or IGF I complex, whereas significantly lower rates were obtained with spermatozoa capacitated in the presence of IGF-I complex.
Minelli
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A
Dipartimento di Scienze Biochimiche e Biotecnologie Molecolari, Sezione di Biochimica Cellulare, Perugia, 06123 Italy. albami@tin.it
Liguori
L
L
Collodel
G
G
Lattaioli
P
P
Castellini
C
C
D020101:Acrosome Reaction / Q000502:physiology; D000818:Animals; D002352:Carrier Proteins / Q000737:chemistry / Q000494:pharmacology; D002454:Cell Differentiation; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D008297:Male; D011817:Rabbits; D012661:Semen / Q000737:chemistry; D013075:Sperm Capacitation / Q000502:physiology*; D013094:Spermatozoa / Q000502:physiology*
D002352:Carrier Proteins; D007334:Insulin-Like Growth Factor I