[Experimental study on inhibitory effect of ganlu xiaodu Dan on coxackie virus in vitro].
OBJECTIVE
To observe the Coxackie virus inhibitory effect of Ganlu Xiaodu Dan (GLXDD), including complete recipe, incomplete recipe and supplemented recipe of it.


METHODS
Using tissue culture technique, the maximal non-toxic concentration of drug on cultured cells, the influence of drug on proliferation of virus in cultured cells and the virus inhibitory effect in different time of action were observed.


RESULTS
The maximal non-toxic concentration of GLXDD was 1:40 (25 g/L), no matter whether the recipe was complete, incomplete or supplementary. CLXDD in that concentration could inhibit significantly the proliferation of Coxackie virus B2, B3 and B4 strains, the inhibition index being over 2 and the output of these strains treated with complete recipe of GLXDD was lower significantly than that of the control group (P < 0.05).


CONCLUSIONS
GLXDD, including its complete recipe, incomplete recipe and supplementary recipe, could inhibit the replication of Coxackie virus in cultured cells.
He
Y
Y
Hunan College of TCM, Changsha 410007.
Wu
C
C
Zhao
G
G
D000998:Antiviral Agents / Q000494:pharmacology*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D029822:Enterovirus B, Human / Q000187:drug effects*; D006801:Humans; D007822:Laryngeal Neoplasms / Q000473:pathology; D014407:Tumor Cells, Cultured
D000998:Antiviral Agents; D004365:Drugs, Chinese Herbal