Isolation of aflatoxigenic strains of Aspergillus and detection of aflatoxin B1 from feeds in India.
In a preliminary study, 256 feed samples collected from different parts of Northern India were examined for the presence of aflatoxigenic strains of Aspergillus favus/parasiticus and for detection of Aflatoxin B1 (AFB1). Out of 198 A. flavus and 15 A. parasiticus strains isolated, 76% and 86% respectively, were found to be toxigenic. Aflatoxin B1 content of these feeds, as estimated by thin layer chromatography (TLC) and enzyme linked immunosorbent assay (ELISA) were very high (average 0.412+/-0.154 ppm) in comparison to the permissible Indian regulation level (0.03 ppm). Seasonal variation of incidence and level of toxin in feed was recorded and it was high during monsoon/post monsoon period.
Dutta
T K
TK
Indian Veterinary Research Institute, Izatnagar.
Das
P
P
D016604:Aflatoxin B1 / Q000032:analysis*; D000821:Animal Feed / Q000032:analysis / Q000382:microbiology*; D000818:Animals; D001230:Aspergillus / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism; D001231:Aspergillus flavus / Q000302:isolation & purification / Q000378:metabolism; D007194:India
D016604:Aflatoxin B1