[Effect of substance P on the short-circuit current of nasal mucosal epithelium].
OBJECTIVE
To observe the changes of epithelial apical membrane short-circuit current (Isc) of antigen-sensitized rat nasal mucosa with the stimulation of Substances P (SP).


METHODS
The epithelial apical membrane Isc were measured with Ussing chamber technique, and and we observed the blockage to the effect of SP by neurokinin receptor antagonist Cp96345, histamine H1 receptor antagonist pyrilamine, H2 antagonist ranitidine and neurotoxin respectively.


RESULTS
The Isc increased significantly with the stimulation of SP. The effect of SP on Isc could be blocked significantly by pretreatment with four substances.


CONCLUSIONS
SP releasing from nervous endings plays the role on elicit a series of pathological changes like enhanced Isc, epithelial permeabilities, etc.
Liu
T
T
Department of Otolaryngology, First Affiliated Hospital, Shanxi Medical University, Taiyuan, 030001.
Yang
P
P
Wang
B
B
D000818:Animals; D001713:Biphenyl Compounds / Q000494:pharmacology; D004594:Electrophysiology; D004848:Epithelium / Q000503:physiopathology; D006634:Histamine H1 Antagonists / Q000494:pharmacology; D006635:Histamine H2 Antagonists / Q000494:pharmacology; D008297:Male; D009297:Nasal Mucosa / Q000503:physiopathology*; D010047:Ovalbumin; D011738:Pyrilamine / Q000494:pharmacology; D011899:Ranitidine / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012221:Rhinitis, Allergic, Perennial / Q000139:chemically induced / Q000503:physiopathology*; D013373:Substance P / Q000037:antagonists & inhibitors*; D013779:Tetrodotoxin / Q000494:pharmacology
D001713:Biphenyl Compounds; D006634:Histamine H1 Antagonists; D006635:Histamine H2 Antagonists; D013373:Substance P; D013779:Tetrodotoxin; D011899:Ranitidine; D010047:Ovalbumin; D011738:Pyrilamine; C066892:CP 96345