[Study on the methodology of potassium channel expression in Xenopus oocytes by messenger RNA from rat cochlear nucleus].
OBJECTIVE
To study the properties of voltage-dependent ion channels expressed in Xenopus oocytes by mRNA from rat cochlear nucleus and to lay a methodological foundation for studying the changes of ion channels in auditory centre with sensorineural hearing loss.


METHODS
Poly (A)+ mRNAs isolated from the rat cochlear nucleus by acid guanidinum thiocyanate-phenol-chloroform (AGPC) method and Promega's new magnesphere technique were microinjected into Xenopus laevis oocytes to express functional ion channels. Voltage clamp technique was used to record the current of voltage-dependent ion channels.


RESULTS
A voltage-dependent ion channel was detected from oocytes injected with rat cochlear nucleus mRNA. The maximum amplitude of the outward current was 364 +/- 42 nA (n = 16). It was identified as transient outward potassium channel.


CONCLUSIONS
The model set up by the test may play an important role in studying the physiology, pathology and pharmacology of auditory centre.
Yang
W
W
PLA General Hospital, Beijing 100853.
Jiang
S
S
Yang
W
W
D000818:Animals; D017626:Cochlear Nucleus / Q000737:chemistry*; D005260:Female; D008297:Male; D008845:Microinjections; D009865:Oocytes / Q000166:cytology / Q000378:metabolism*; D015221:Potassium Channels / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification; D051381:Rats; D017208:Rats, Wistar; D014982:Xenopus laevis
D015221:Potassium Channels; D012333:RNA, Messenger