Enhanced synthesis of choline and glycine betaine in transgenic tobacco plants that overexpress phosphoethanolamine N-methyltransferase.
Choline (Cho) is the precursor of the osmoprotectant glycine betaine and is itself an essential nutrient for humans. Metabolic engineering of Cho biosynthesis in plants could therefore enhance both their resistance to osmotic stresses (drought and salinity) and their nutritional value. The key enzyme of the plant Cho-synthesis pathway is phosphoethanolamine N-methyltransferase, which catalyzes all three of the methylations required to convert phosphoethanolamine to phosphocholine. We show here that overexpressing this enzyme in transgenic tobacco increased the levels of phosphocholine by 5-fold and free Cho by 50-fold without affecting phosphatidylcholine content or growth. Moreover, the expanded Cho pool led to a 30-fold increase in synthesis of glycine betaine via an engineered glycine betaine pathway. Supplying the transgenics with the Cho precursor ethanolamine (EA) further enhanced Cho levels even though the supplied EA was extensively catabolized. These latter results establish that there is further scope for improving Cho synthesis by engineering an increased endogenous supply of EA and suggest that this could be achieved by enhancing EA synthesis and/or by suppressing its degradation.
McNeil
S D
SD
Horticultural Sciences Department, University of Florida, Gainesville 32611-0690, USA.
Nuccio
M L
ML
Ziemak
M J
MJ
Hanson
A D
AD
D001622:Betaine / Q000378:metabolism*; D002736:Chloroplasts / Q000201:enzymology; D002794:Choline / Q000096:biosynthesis*; D019856:Ethanolamine / Q000378:metabolism; D004983:Ethanolamines / Q000378:metabolism; D005813:Genes, Synthetic; D005822:Genetic Vectors / Q000235:genetics; D008745:Methylation; D008780:Methyltransferases / Q000235:genetics / Q000378:metabolism*; D008954:Models, Biological; D009029:Mosaic Viruses / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D013045:Species Specificity; D018724:Spinacia oleracea / Q000235:genetics; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism*; D019076:Transgenes
D004983:Ethanolamines; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; D001622:Betaine; D019856:Ethanolamine; C005448:phosphorylethanolamine; D008780:Methyltransferases; C102844:phosphoethanolamine methyltransferase; D002794:Choline