Expression of the rabbit cytochrome P450 aromatase encoding gene uses alternative tissue-specific promoters.
The aim of the present study was to analyse the tissue-specific expression of various promoter-derived transcripts from the gene encoding rabbit aromatase cytochrome P450. A new promoter, named I.r, was identified, and promoters II and I.r were sequenced. Promoter I.r-derived transcripts were found in preovulatory granulosa cells, corpus luteum, placenta and adipose tissue. An alternative splice variant of this transcript was found with tissue-specific preference. Tissue-specific expression of promoter-derived variants was studied in the ovary before and after ovulation. While the level of promoter II-derived transcript decreased dramatically after ovulation, that of promoter I.r-derived transcript remained unchanged, indicating that promoter II and promoter I.r were not controlled by a single regulation system. The existence of this dual system of regulation suggests that the rabbit ovary could be a useful model to study the promoter-specific regulation of aromatase.
Bouraïma
H
H
Laboratoire de Biochimie, UPRES EA 2608, USC INRA, CHU Côte de Nacre, Université de Caen, France. helene.bouraima@wanadoo.fr
Hanoux
V
V
Mittre
H
H
Féral
C
C
Benhaïm
A
A
Leymarie
P
P
D017398:Alternative Splicing; D000818:Animals; D001141:Aromatase / Q000235:genetics*; D001483:Base Sequence; D005260:Female; D008969:Molecular Sequence Data; D009928:Organ Specificity; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000032:analysis; D011817:Rabbits; D016385:TATA Box
D012333:RNA, Messenger; D001141:Aromatase