Systematic analysis of acid, neutral and basic drugs in horse plasma by combination of solid-phase extraction, non-aqueous partitioning and gas chromatography-mass spectrometry.
A sample preparation method for mass chromatographic detection of doping drugs from horse plasma is described. Bond Elut Certify (1 g/6 ml) is used for the extraction of 4 ml of horse plasma. Fractionation is performed with 6 ml of CHCl3-Me2CO (8:2) and 5 ml of 1% TEA-MeOH according to its property. Simple and effective clean-up based on non-aqueous partitioning is adopted to remove co-eluted contaminants in both acid and basic fractions. Two kinds of 1-(N,N-diisopropylamino)-n-alkanes are co-injected with the sample into the GC-MS system for the calculation of the retention index. Total recoveries of 107 drugs are examined. Some data of post administration plasma are presented. This procedure achieves sufficient recoveries and clean extracts for GC-MS analysis. The method is able to detect ng/ml drug levels in horse plasma.
Takeda
A
A
Racing Chemistry Laboratory, Utsunomiya, Tochigi, Japan. a-takeda@lrc.or.jp
Tanaka
H
H
Shinohara
T
T
Ohtake
I
I
D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D004300:Doping in Sports; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006736:Horses / Q000097:blood*; D006863:Hydrogen-Ion Concentration; D004364:Pharmaceutical Preparations / Q000097:blood*
D004364:Pharmaceutical Preparations