Detection of the isoflavone aglycones genistein and daidzein in urine using solid-phase microextraction-high-performance liquid chromatography-electrospray ionization mass spectrometry.
An improved method of detection of the isoflavone aglycones, genistein and daidzein, is reported using solid-phase microextraction-high-performance liquid chromatography-electrospray ionization mass spectrometry (SPME-HPLC-ESI-MS). Extraction of the isoflavonoids from urine using SPME with a Carbowax-templated resin fiber coating allows rapid preconcentration of the analytes without the usual sample preparation required by other methods. Detection of the analytes is accomplished by HPLC-ESI-MS. Analysis of spiked samples of urine resulted in a linear range of 0.25 to 250 ng/ml for daidzein and 0.27 to 27.0 ng/ml for genistein. Limits of detection of daidzein and genistein were measured at 25.4 pg/ml for daidzein and 2.70 pg/ml for genistein. Daidzein and genistein were detected in urine following consumption of a soy drink.
Satterfield
M
M
Department of Chemistry and Biochemistry, University of Texas at Austin, 78712, USA.
Black
D M
DM
Brodbelt
J S
JS
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D019833:Genistein / Q000652:urine*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007529:Isoflavones / Q000652:urine*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D007529:Isoflavones; C004742:daidzein; D019833:Genistein