title: Implantation of silicon chip microphotodiode arrays into the cat subretinal space.
abstract: There are currently no therapies to restore vision to patients blinded by photoreceptor degeneration. This project concerns an experimental approach toward a semiconductor-based subretinal prosthetic designed to electrically stimulate the retina. The present study describes surgical techniques for implanting a silicon microphotodiode array in the cat subretinal space and subsequent studies of implant biocompatibility and durability. Using a single-port vitreoretinal approach, implants were placed into the subretinal space of the right eye of normal cats. Implanted retinas were evaluated post-operatively over a 10 to 27 month period using indirect ophthalmoscopy, fundus photography, electroretinography, and histology. Infrared stimulation was used to isolate the electrical response of the implant from that of the normal retina. Although implants continued to generate electrical current in response to light, the amplitude of the implant response decreased gradually due to dissolution of the implant's gold electrode. Electroretinograms recorded from implanted eyes had normal waveforms but were typically 10-15% smaller in amplitude than those in unimplanted left eyes. The nonpermeable silicon disks blocked choroidal nourishment to the retina, producing degeneration of the photoreceptors. The laminar structure of the inner retinal layers was preserved. Retinal areas located away from the implantation site appeared normal in all respects. These results demonstrate that silicon-chip microphotodiode-based implants can be successfully placed into the subretinal space. Gold electrode-based subretinal implants, however, appear to be unsuitable for long-term use due to electrode dissolution and subsequent decreased electrical activity.
authors.affiliation: Optobionics Corporation, Wheaton, IL 60187, USA. alanykc@aol.com
authors.forename: A Y
authors.initials: AY
authors.lastname: Chow
authors.forename: M T
authors.initials: MT
authors.lastname: Pardue
authors.forename: V Y
authors.initials: VY
authors.lastname: Chow
authors.forename: G A
authors.initials: GA
authors.lastname: Peyman
authors.forename: C
authors.initials: C
authors.lastname: Liang
authors.forename: J I
authors.initials: JI
authors.lastname: Perlman
authors.forename: N S
authors.initials: NS
authors.lastname: Peachey
mesh_terms: D000818:Animals; D001766:Blindness / Q000473:pathology / Q000503:physiopathology* / Q000534:rehabilitation*; D002415:Cats; D004195:Disease Models, Animal; D004558:Electric Stimulation; D004567:Electrodes, Implanted; D004594:Electrophysiology; D004596:Electroretinography; D009887:Ophthalmoscopy; D020419:Photoreceptor Cells, Vertebrate / Q000473:pathology / Q000502:physiology; D011474:Prosthesis Design / Q000295:instrumentation; D019919:Prosthesis Implantation; D012160:Retina / Q000473:pathology / Q000503:physiopathology* / Q000601:surgery*; D012164:Retinal Diseases / Q000473:pathology / Q000503:physiopathology* / Q000534:rehabilitation*; D012825:Silicon / Q000627:therapeutic use*
chemical_list: D012825:Silicon