[Analysis of fibronectin and laminin in the extracellular matrixes of acute myeloid leukemia and its implications].
OBJECTIVE
To investigate the relationship between quantitative change of fibronectin (Fn) or Laminin (Lm) and growth and differentiation of leukemic cells.


METHODS
The extracellular matrixes, Fn and Lm, in long-term bone marrow culture (LTBMC) from 18 acute myeloid leukemia (AML) patients and 6 normal subjects were examined by immunofluorescence assay.


RESULTS
1. The Fn or Lm content in 9 AML self-maintained (AMLsm) 1-3 week-cultures was significantly less than that in 9 AML not self-maintained (AMLnsm) cultures. 2. In the third week's cultures, the blasts of 8 AMLnsms decreased to less than 5%, but did not of 7 AMLsms. 3. As the cultures going on, the blasts were related to Fn or Lm contents positively in normal control and negatively in AMLnsm. The blasts were related to Fn contents negatively in AMLsm.


CONCLUSIONS
The change of Fn or Lm may play an important role in the development of AML. In addition, the difference of Fn or Lm contents between AMLsm and AMLnsm was helpful to the choice of AML patient for in vitro purging of leukemic cells through LTBMC before auto-BMT.
Lin
Y
Y
Fujian Institute of Hematology, Fuzhou 350001.
Lu
L
L
Chen
Z
Z
D000293:Adolescent; D000328:Adult; D016465:Bone Marrow Purging; D005109:Extracellular Matrix / Q000378:metabolism*; D005260:Female; D005353:Fibronectins / Q000378:metabolism*; D006801:Humans; D007797:Laminin / Q000378:metabolism*; D015470:Leukemia, Myeloid, Acute / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D014407:Tumor Cells, Cultured
D005353:Fibronectins; D007797:Laminin