title: Transdentinal stimulation of reactionary dentinogenesis in ferrets by dentine matrix components.
abstract: OBJECTIVE
  Trans-dentinal stimulation of reactionary dentinogenesis may be mediated through cellular signalling by bio-active components released from the dentine matrix during injury. Understanding of these processes will be important to guide dentinal repair activity following restorative surgery. The purpose of this study was to investigate the effects of implanting isolated dentine matrix proteins within cavity preparations on dentinal repair activity and odontoblast survival using a controlled experimental animal model.
  
  
  METHODS
  Forty-five ferret canine teeth each had a standardised non-exposed cylindrical Class V cavity cut into the buccal dentine. Ten cavities were restored with zinc oxide eugenol, as a control. Two different lyophilised preparations of dentine matrix components were implanted on the axial floor of the remaining 35 cavities prior to filling with zinc oxide eugenol. After post-operative periods of 2, 7, 14, 28, and 90 days, the teeth were extracted and examined histomorphometrically and the data analysed statistically by analysis of variance tests.
  
  
  RESULTS
  The odontoblasts beneath the restored cavities responded to the presence of the two dentine matrix preparations by increasing the mean area of reactionary dentine secreted by 433 and 578%, and the numbers of odontoblasts remained stable.
  
  
  CONCLUSIONS
  Dentine matrix components can stimulate reactionary dentinogenesis in non-exposed cavity preparations. It will now be important to identify how this may be harnessed as a part of routine restorative surgery to optimise treatment outcomes with a biological basis.
authors.affiliation: Oral Biology, School of Dentistry, The University of Birmingham, Birmingham B4 6NN, UK. a.j.smith@bham.ac.uk
authors.forename: A J
authors.initials: AJ
authors.lastname: Smith
authors.forename: R S
authors.initials: RS
authors.lastname: Tobias
authors.forename: P E
authors.initials: PE
authors.lastname: Murray
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D003737:Dental Cavity Preparation; D003804:Dentin / Q000737:chemistry / Q000502:physiology*; D003809:Dentin, Secondary / Q000254:growth & development / Q000378:metabolism*; D016326:Extracellular Matrix Proteins / Q000494:pharmacology* / Q000502:physiology; D005289:Ferrets; D023421:Models, Animal; D009804:Odontoblasts / Q000187:drug effects; D011817:Rabbits
chemical_list: D016326:Extracellular Matrix Proteins