title: Liquid chromatographic determination of nystatin in pharmaceutical preparations.
abstract: A rapid, reversed-phase liquid chromatographic method was developed for the assay of nystatin in the bulk drug and a variety of dosage forms. Analysis was performed on a Symmetry C18 reversed-phase column using a mobile phase of methanol-water-dimethylformamide (DMF; 55 + 30 + 15, v/v/v), with detection by UV at 305 nm. Quantitation is based on the sum of the peak areas of the 2 major isomers of nystatin. The linearity of the assay was determined for a concentration range of 0.05 to 0.2 mg/mL (correlation coefficient > 0.999). Accuracies and precision showed good reproducibility.
authors.affiliation: US Food and Drug Administration, NRL, Jamaica, NY 11433, USA.
authors.forename: P
authors.initials: P
authors.lastname: Wilson
authors.forename: A
authors.initials: A
authors.lastname: Stewart
authors.forename: V
authors.initials: V
authors.lastname: Flournoy
authors.forename: S W
authors.initials: SW
authors.lastname: Zito
authors.forename: A
authors.initials: A
authors.lastname: Vancura
mesh_terms: D000935:Antifungal Agents / Q000032:analysis*; D002853:Chromatography, Liquid; D009761:Nystatin / Q000032:analysis*; D004364:Pharmaceutical Preparations
chemical_list: D000935:Antifungal Agents; D004364:Pharmaceutical Preparations; D009761:Nystatin