Determination of ketobemidone and its metabolites in plasma and urine using solid-phase extraction and liquid chromatography-mass spectrometry.
The authors developed a sensitive, specific, and rapid liquid chromatography--mass spectrometry (LC-MS) method for determining ketobemidone and its major metabolites in plasma and urine. The method involves a solid-phase extraction, high-performance liquid chromatography (HPLC), and electrospray mass spectrometry. The limit of quantification for ketobemidone and norketobemidone was 3 nmol/L. Recovery rates for ketobemidone and norketobemidone were 84.8% and 81.1%, respectively. Coefficients of variation (CV) ranged from 2.8 % to 9.5%. The method was used to determine ketobemidone and its major metabolites in clinical samples from relevant patient groups.
Svensson
J O
JO
Department of Medical Laboratory Sciences and Technology, Division of Clinical Pharmacology, Huddinge University Hospital, Stockholm, Sweden.
Säwe
J
J
AL-Shurbaji
A
A
D000701:Analgesics, Opioid / Q000097:blood* / Q000652:urine*; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D008614:Meperidine / Q000031:analogs & derivatives / Q000097:blood* / Q000652:urine*; D011786:Quality Control; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D015813:Substance Abuse Detection
D000701:Analgesics, Opioid; D008614:Meperidine; C012394:ketobemidone