[Study on the expression of calreticulin in hypertrophic scar-derived fibroblasts].
OBJECTIVE
To investigate the expression level and intracellular distribution of calreticulin(CRT) in hypertrophic scar-derived fibroblasts(HSF) and normal skin-derived fibroblasts(NSF).


METHODS
Using anti-CRT antibody, CRT was detected in HSF and NSF by immunocytochemistry and ELISA technique.


RESULTS
CRT was present in the cytoplasm and nuclei in proliferating HSF or NSF in culture, but HSF possessed significantly higher expression level of CRT than NSF(P < 0.01). When these cells (HSF and NSF) were fused, neither intracellular nor intranuclear staining for CRT was observed.


CONCLUSIONS
In HSF and NSF, the distribution of CRT is widespread. Because of its abundant expression in HSF, we postulate that CRT as a multifunctional protein plays an important role in Ca2+ sequestering, integrin-mediated signalling and cell adhesion.
Zhao
Y
Y
Department of Plastic Surgery, Navy 411 Hospital, Shanghai 200081.
He
Q
Q
D000293:Adolescent; D000328:Adult; D002135:Calcium-Binding Proteins / Q000096:biosynthesis*; D037282:Calreticulin; D002478:Cells, Cultured; D017439:Cicatrix, Hypertrophic / Q000378:metabolism* / Q000473:pathology; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D037102:Lectins / Q000096:biosynthesis; D012261:Ribonucleoproteins / Q000096:biosynthesis*; D012867:Skin / Q000166:cytology / Q000378:metabolism*
D002135:Calcium-Binding Proteins; D037282:Calreticulin; D037102:Lectins; D012261:Ribonucleoproteins