[Cellular compatibility of three natural xenogeneic bone derived biomaterials].
OBJECTIVE
To evaluate the cellular compatibility of three natural xenogeneic bone derived biomaterials.


METHODS
Three types of natural xenogeneic bone derived biomaterials were made with physical and chemical treatment, composite fully deproteinized bone(CFDB), partially deproteinized bone(PDPB) and partially decalcified bone(PDCB). Three types biomaterials were cocultured with human embryonic periosteal osteoblasts. The cell growth, attachment, cell cycle, alkaline phosphatase activity were detected to evaluate the cellular compatibility to biomaterials.


RESULTS
Osteoblasts attached on all three biomaterials and grew well, the effect of three biomaterials on cell proliferation was PDCB > PDPB > CFDB. The cell cycle was not obviously affected by three biomaterials. The effect of three biomaterials on alkaline phosphatase activity of osteoblasts was PDCB > PDPB > CFDB.


CONCLUSIONS
CFDB,PDPB,PDCB have good cellular compatibility without cytotoxic and tumorigenicity, CFDB is the best. The three biomaterials can be used as scaffold materials of bone tissue engineering.
Li
Y L
YL
Department of Orthopedic Surgery, First University Hospital, West China University of Medical Sciences, Chengdu Sichuan, P. R. China 610041.
Yang
Z M
ZM
Xie
H Q
HQ
D018786:Bone Substitutes; D002454:Cell Differentiation / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D006648:Histocompatibility; D006801:Humans; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology; D023822:Tissue Engineering
D018786:Bone Substitutes