title: Temporal changes in natriuretic and antinatriuretic systems after closure of a large arteriovenous fistula.
abstract: OBJECTIVE
  Surgical closure of a large arteriovenous (A-V) fistula in patients and animals is associated with prompt diuresis and natriuresis. However, the mechanisms underlying these changes remained largely unknown.
  
  
  METHODS
  The present study evaluated the hormonal balance between major antinatriuretic systems (plasma renin activity, PRA, and arginine vasopressin, AVP) and natriuretic systems (atrial natriuretic peptide, ANP, and renal nitric oxide, NO) in Wistar rats with an A-V fistula (1.2 mm O.D., side to side) between the abdominal aorta and inferior vena cava.
  
  
  RESULTS
  The placement of an A-V fistula caused progressive sodium retention (UNaV decreased from 1500 to 100 microequiv./day), a significant drop in mean arterial blood pressure (MAP) from 127+/-3 to 75+/-2 mmHg (P<0.01), and a significant increase in ANP (from 94+/-12 to 389+/-135 pg/ml, P<0.05), PRA (from 22.1+/-2.0 to 47+/-14 ng angiotensin I [Ang I]/ml/h, P<0.05), AVP (from 14.2+/-3.6 to 37.7+/-9.6 pg/ml, P<0.05), norepinephrine (from 184.2+/-40.5 to 1112.6+/-293.2 pg/ml, P<0.05) and epinephrine (from 667.5+/-175.9 to 2049.8+/-496.9 pg/ml, P<0.05). Furthermore, these changes were associated with a 3-fold increase in the renal medullary immunoreactive levels of endothelial NO synthase (eNOS), an endogenous vasodilator that plays an important role in the regulation of medullary blood flow. After 6 days, rats with A-V fistula and maximal sodium retention underwent surgical closure of the A-V fistula. The A-V fistula closure was associated with dramatic natriuresis (UNaV=2563+/-78 and 1918+/-246 microEq/day on days 3 and 6 following the closure, respectively) and restoration of MAP to normal levels (111+/-6 mmHg); PRA decreased to 29+/-5 ng Ang I/ml/h, AVP to 20.3+/-7.1 pg/ml, and medullary eNOS declined to basal levels, whereas plasma ANP concentrations remained elevated (380+/-90 pg/ml) after 3 days and returned to normal (92+/-12 pg/ml) on day 6.
  
  
  CONCLUSIONS
  These results demonstrate that the creation of A-V fistula is associated with activation of both natriuretic and antinatriuretic systems. Closure of A-V fistula is characterized by shifting the balance in favor of the natriuretic substances. Moreover, the observed alterations in medullary eNOS following the creation and closure of A-V fistula suggest that this system, an important determinant of medullary blood flow, may contribute significantly to the regulation of sodium excretion in this model.
authors.affiliation: Department of Physiology and Biophysics, The Bruce Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, P.O.B. 9649, 31096, Haifa, Israel.
authors.forename: Z A
authors.initials: ZA
authors.lastname: Abassi
authors.forename: S
authors.initials: S
authors.lastname: Brodsky
authors.forename: T
authors.initials: T
authors.lastname: Karram
authors.forename: I
authors.initials: I
authors.lastname: Dobkin
authors.forename: J
authors.initials: J
authors.lastname: Winaver
authors.forename: A
authors.initials: A
authors.lastname: Hoffman
mesh_terms: D000818:Animals; D001127:Arginine Vasopressin / Q000097:blood; D001166:Arteriovenous Shunt, Surgical; D009320:Atrial Natriuretic Factor / Q000097:blood; D001794:Blood Pressure / Q000502:physiology; D006333:Heart Failure / Q000378:metabolism / Q000503:physiopathology*; D006728:Hormones / Q000097:blood; D007668:Kidney / Q000201:enzymology; D008297:Male; D009318:Natriuresis / Q000502:physiology*; D019001:Nitric Oxide Synthase / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012083:Renin / Q000097:blood; D012964:Sodium / Q000652:urine; D014556:Urine
chemical_list: D006728:Hormones; D001127:Arginine Vasopressin; D009320:Atrial Natriuretic Factor; D012964:Sodium; D019001:Nitric Oxide Synthase; D012083:Renin