Functional analysis of B144/LST1: a gene in the tumor necrosis factor cluster that induces formation of long filopodia in eukaryotic cells.
B144/LST1 is a gene encoded in the human major histocompatibility complex that produces multiple forms of alternatively spliced mRNA and encodes peptides fewer than 100 amino acids in length. B144/LST1 is strongly expressed in dendritic cells. Transfection of B144/LST1 into a variety of cells induces morphologic changes including the production of long, thin filopodia differing from those seen on transfection of a dominant active CDC42 gene. The structures are dynamically rearranging and sometimes connect one cell with another. The full effect of B144/LST1 protein on cell morphology requires the retention of at least one of the four cysteines of the peptide plus the presence of a hydrophobic segment in the protein, but requires only one of the two coding regions present in the terminal 3' exons.
Raghunathan
A
A
Boyer Center for Molecular Medicine, Room 333, Department of Genetics, Yale School of Medicine, 295 Congress Avenue, New Haven, Connecticut 06536, USA.
Sivakamasundari
R
R
Wolenski
J
J
Poddar
R
R
Weissman
S M
SM
D000595:Amino Acid Sequence; D000818:Animals; D001798:Blood Proteins / Q000096:biosynthesis / Q000235:genetics*; D019556:COS Cells; D002454:Cell Differentiation; D002455:Cell Division; D017124:Conserved Sequence; D003594:Cytoplasmic Granules; D003713:Dendritic Cells / Q000166:cytology; D019143:Evolution, Molecular; D006801:Humans; D007107:Immune System / Q000166:cytology; D047908:Intracellular Signaling Peptides and Proteins; D008285:Major Histocompatibility Complex / Q000235:genetics*; D008565:Membrane Proteins; D051379:Mice; D009024:Morphogenesis; D005810:Multigene Family; D009419:Nerve Tissue Proteins / Q000378:metabolism; D011554:Pseudopodia / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*; D051317:Wiskott-Aldrich Syndrome Protein, Neuronal; D020764:cdc42 GTP-Binding Protein / Q000378:metabolism
D001798:Blood Proteins; D047908:Intracellular Signaling Peptides and Proteins; C093604:LST1 protein, human; C490562:Lst1 protein, mouse; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; C495771:WASL protein, human; C495772:Wasl protein, mouse; D051317:Wiskott-Aldrich Syndrome Protein, Neuronal; D020764:cdc42 GTP-Binding Protein