title: DNA sequence and physical mapping of the canine transglutaminase 1 gene.
abstract: The transglutaminase 1 gene (TGM1) encodes an enzyme necessary for cross-linking the structural proteins that form the cornified envelope, an essential component of the outermost layer of the skin, the stratum corneum. Reported here is the complete coding region of canine TGM1, its chromosome localization, and its map position in the integrated canine linkage-radiation hybrid map. Canine TGM1 consists of 2,448 nucleotides distributed over 15 exons. The nucleotide sequence has 90% identity to human TGM1. The deduced canine TGM1 protein is 816 amino acids long and is 92% identical to human TGM1. Using fluorescence in situ hybridization, we localized canine TGM1 to dog (Canis familiaris) chromosome 8 (CFA 8q). Canine TGM1 localized to CFA 8 on the integrated linkage-radiation hybrid map in the interval FH2149-MYH7. Characterizing the coding region of canine TGM1 is a first step in examining the role of this enzyme in normal and defective cornification in the dog.
authors.affiliation: Department of Veterinary Pathobiology, Texas A&M University, College Station, TX 77843-4467, USA. kcredille@cvm.tamu.edu
authors.forename: K M
authors.initials: KM
authors.lastname: Credille
authors.forename: P J
authors.initials: PJ
authors.lastname: Venta
authors.forename: M
authors.initials: M
authors.lastname: Breen
authors.forename: J K
authors.initials: JK
authors.lastname: Lowe
authors.forename: K E
authors.initials: KE
authors.lastname: Murphy
authors.forename: E A
authors.initials: EA
authors.lastname: Ostrander
authors.forename: F
authors.initials: F
authors.lastname: Galibert
authors.forename: R W
authors.initials: RW
authors.lastname: Dunstan
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004283:Dog Diseases / Q000201:enzymology / Q000235:genetics*; D004285:Dogs; D005091:Exons / Q000235:genetics; D008040:Genetic Linkage / Q000235:genetics; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007438:Introns / Q000235:genetics; D008969:Molecular Sequence Data; D020161:Physical Chromosome Mapping; D021101:Radiation Hybrid Mapping; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012871:Skin Diseases / Q000201:enzymology / Q000235:genetics / Q000662:veterinary; D011503:Transglutaminases / Q000235:genetics*
chemical_list: D011503:Transglutaminases; C105804:transglutaminase 1