Functional HIV CXCR4 coreceptor on human epithelial Langerhans cells and infection by HIV strain X4.
HIV can cross the intact epithelium of genital mucosae via Langerhans cells. Fresh Langerhans cells are known to express CD4 and CCR5. The presence of CXCR4 on the surface of cultured but not freshly isolated Langerhans cells has been described. In the present study, we demonstrate that CXCR4 was expressed by fresh Langerhans cells isolated and purified from epidermis. However, the percentage of Langerhans cells expressing CXCR4 or CCR5 increased during maturation of the cells in culture, especially in the presence of exogenous granulocyte-macrophage colony-stimulating factor. To determine whether CXCR4 was functional, freshly isolated Langerhans cells were infected with HIV LAI, a T-cell-tropic strain, and p24 protein production was measured in culture supernatants. p24 production was observed when infected Langerhans cells were cocultured with SupT1 cells. However, the presence of HIV provirus DNA was evidenced within the infected Langerhans cells by nested PCR. Ultrastructural studies confirmed the formation of syncytia when Langerhans cells were cocultured with SupT1 cells. Preincubation of Langerhans cells with azidothymidine or SDF-1-alpha, a natural ligand for CXCR4, prevented infection. These data demonstrated that CXCR4 is present on the surface of Langerhans cells freshly isolated from human skin epidermis and that this expression is functional.
Tchou
I
I
Groupe Immunité des Muqueuses et Agents Pathogènes and. Centre de Cytométrie en Flux, University of Saint-Etienne. INSERM U346, Lyon, France.
Misery
L
L
Sabido
O
O
Dezutter-Dambuyant
C
C
Bourlet
T
T
Moja
P
P
Hamzeh
H
H
Peguet-Navarro
J
J
Schmitt
D
D
Genin
C
C
D015704:CD4 Antigens / Q000187:drug effects / Q000378:metabolism; D018920:Coculture Techniques; D004847:Epithelial Cells / Q000378:metabolism / Q000821:virology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D015497:HIV-1 / Q000472:pathogenicity*; D006801:Humans; D016753:Interleukin-10 / Q000494:pharmacology; D007801:Langerhans Cells / Q000378:metabolism / Q000821:virology*; D009092:Mucous Membrane / Q000378:metabolism / Q000821:virology; D019713:Receptors, CCR5 / Q000187:drug effects / Q000378:metabolism; D019718:Receptors, CXCR4 / Q000187:drug effects / Q000378:metabolism*; D015728:Receptors, HIV / Q000187:drug effects / Q000378:metabolism*; D013601:T-Lymphocytes / Q000821:virology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D015704:CD4 Antigens; D019713:Receptors, CCR5; D019718:Receptors, CXCR4; D015728:Receptors, HIV; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D016178:Granulocyte-Macrophage Colony-Stimulating Factor