Radioimmunoguided breast surgery using radiolabeled antibody NR-LU-10 FAB: a pilot study.
OBJECTIVE
Radioimmunoguided surgery using radiolabeled NR-LU-10 Fab was evaluated as a method of intraoperative breast cancer detection.


METHODS
Breast cancer patients were injected intravenously with 125I (74 MBq) labeled NR-LU-10 Fab (5 mg) and then underwent tumor excision 2, 4 or 7 days later, during which time the gamma detector probe was used to evaluate the primary tumor for evidence of radioactive uptake.


RESULTS
Intraoperative probing revealed tumor localization in 7 of 10 patients (70%). Gamma probe counts of the excised tumor were elevated in all patients, although high counts in surrounding non-malignant tissue obscured the ability to detect the tumor in vivo in 3 patients. One patient with bilateral breast cancer was found to have a separate focus of occult tumor in each breast using the gamma detector probe.


CONCLUSIONS
Radiolabeled NR-LU-10 Fab possesses favorable pharmokinetics and tumor-binding ability as a targeting agent. However, binding to non-malignant tissue limits its role in the intraoperative evaluation of tumor margins in breast cancer patients. Its role in other malignancies should be explored.
Burak
W E
WE
Department of Surgery, The Ohio State University Comprehensive Cancer Center, Arthur G James Cancer Hospital and Richard J Solove Research Institute, Columbus, USA. burak.1@osu.edu
DePalatis
L
L
Mosic
J L
JL
Soble
D
D
Hinkle
G
G
Hitchcock
C L
CL
D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal / Q000493:pharmacokinetics; D001943:Breast Neoplasms / Q000097:blood / Q000000981:diagnostic imaging* / Q000601:surgery* / Q000652:urine; D005260:Female; D006801:Humans; D007267:Injections; D007457:Iodine Radioisotopes; D015412:Mastectomy, Segmental / Q000379:methods*; D008875:Middle Aged; D010865:Pilot Projects; D016719:Radioimmunodetection / Q000379:methods*; D013997:Time Factors
D000911:Antibodies, Monoclonal; D007457:Iodine Radioisotopes; C405617:NR-LU-10 antibody