title: Cell growth and by-product formation in a pyruvate kinase mutant of E. coli.
abstract: In this paper, we report on the analysis of acid formation in an E. coli pyk mutant. The results demonstrate that acid formation is insignificant for both the wild-type and the mutant at low glucose concentrations. However, at relatively high glucose concentrations, acid formation remains very low for the mutant but is significant for the wild-type. This substantial reduction in acids is accompanied by an increase in CO(2) production. Moreover, unlike the B. subtilis pyk mutant, the E. coli pyk mutant did not show a substantial increase in the PEP pool.
authors.affiliation: Department of Chemical Engineering, University of Pittsburgh, Pittsburgh, Pennsylvania 15219, USA.
authors.forename: T
authors.initials: T
authors.lastname: Zhu
authors.forename: C
authors.initials: C
authors.lastname: Phalakornkule
authors.forename: R R
authors.initials: RR
authors.lastname: Koepsel
authors.forename: M M
authors.initials: MM
authors.lastname: Domach
authors.forename: M M
authors.initials: MM
authors.lastname: Ataai
mesh_terms: D000143:Acids / Q000378:metabolism; D001412:Bacillus subtilis / Q000235:genetics / Q000378:metabolism; D002245:Carbon Dioxide / Q000378:metabolism; D002455:Cell Division / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D009154:Mutation; D010728:Phosphoenolpyruvate / Q000378:metabolism; D011770:Pyruvate Kinase / Q000235:genetics* / Q000378:metabolism
chemical_list: D000143:Acids; D002245:Carbon Dioxide; D010728:Phosphoenolpyruvate; D011770:Pyruvate Kinase; D005947:Glucose