[Evaluation of lipid peroxidation and acid-base status in cord blood of newborns after diabetes in pregnancy].
The purpose of our study was to evaluate lipid peroxidation products and scavenging enzyme activity in placenta and cord blood as well as the estimation of acid-base status and blood gases. Seventy five pregnant patients and their newborns were investigated. Twenty eight had pre-gestational diabetes mellitus (PGDM) and 19 gestational diabetes mellitus (GDM). The following parameters were measured: malondialdehyde (MDA) concentrations, glutathione (GSH) levels, the activity of CuZn dismutase (SOD) (Bioxytech, France). Base excess, pO2, pCO2 and pH were measured in arterial and venous samples. Statistical analysis was performed using Mann-Whitney U test. MDA levels and GSH content increased significantly, while SOD activities declined in diabetic group. Newborns of PDGM mothers had essentially diminished pH and rised both, pCO2 and base deficit. There were no any significant differences in parameters of acid-base balance in newborns of patients with GDM as compared with healthy patients. Our results suggest, that in diabetic patients the fetuses are exposed to increased oxidative stress. The evaluation of antioxidant defence and lipid peroxidation, apart from routine measurement of acid-base balance, might serve as a useful marker of fetal distress in diabetic patients.
Kinalski
M
M
Zakład Patofizjologii Ciazy Akademii Medycznej w Białymstoku.
Sledziewski
A
A
Telejko
B
B
Kowalska
I
I
Kretowski
A
A
Kinalska
I
I
D000137:Acid-Base Imbalance / Q000097:blood / Q000175:diagnosis; D000328:Adult; D015415:Biomarkers / Q000097:blood; D002245:Carbon Dioxide / Q000097:blood; D016640:Diabetes, Gestational / Q000097:blood*; D005260:Female; D005312:Fetal Blood / Q000378:metabolism*; D005316:Fetal Distress / Q000175:diagnosis; D005978:Glutathione / Q000097:blood; D006801:Humans; D007231:Infant, Newborn; D007232:Infant, Newborn, Diseases / Q000097:blood* / Q000175:diagnosis; D015227:Lipid Peroxidation; D008315:Malondialdehyde / Q000097:blood; D018384:Oxidative Stress; D010100:Oxygen / Q000097:blood; D011247:Pregnancy; D011254:Pregnancy in Diabetics / Q000097:blood*; D011297:Prenatal Exposure Delayed Effects; D013482:Superoxide Dismutase / Q000097:blood
D015415:Biomarkers; D002245:Carbon Dioxide; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D005978:Glutathione; D010100:Oxygen