[The regulation of estrogen and tamoxifen to bcl-2 oncogene in endometrial carcinoma].
OBJECTIVE
To study the regulation mechanisms of bcl-2 oncogene in endometrial carcinoma.


METHODS
A reverse transcription-polymerase chain reaction (RT-PCR) method was utilized to quantitate bcl-2 mRNA levels which regulated by estrogen(17-beta-estradiol) and tamoxifen in estrogen receptor (ER)(+) RL-952 endometrial carcinoma cells.


RESULTS
bcl-2 mRNA level was detected in ER(+) RL-952 cells. 17-beta-estradiol stimulated a concentration-dependent increase in bcl-2 mRNA level, while tamoxifen lead to a concentration-dependent decrease in bcl-2 mRNA level.


CONCLUSIONS
bcl-2 oncogene expression in ER(+) endometrial carcinoma is regulated by estrogen and antiestrogen. Estrogen may up-regulate the cell bcl-2 level to inhibit cell apoptosis, while tamoxifen may down-regulate the cell bcl-2 level and induce apoptosis.
Lai
D
D
Zhongshan Hospital of Shanghai Medical University, Shanghai 200032.
Zhu
G
G
Zhou
J
J
D002277:Carcinoma / Q000235:genetics / Q000473:pathology; D016889:Endometrial Neoplasms / Q000235:genetics* / Q000473:pathology; D004965:Estrogen Antagonists / Q000494:pharmacology*; D004967:Estrogens / Q000494:pharmacology*; D005260:Female; D019254:Genes, bcl-2; D006801:Humans; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D013629:Tamoxifen / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D004965:Estrogen Antagonists; D004967:Estrogens; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; D013629:Tamoxifen