Development of an automated in-line microfiltration system coupled to an HPLC for the determination of solubility.
An automated in-line microfiltration system coupled to an HPLC was developed for the determination of solubility. Filtrations and subsequent solubility determinations are performed using a standard Gilson dilutor and autosampler and dual Rheodyne valves equipped with a filtration assembly and configured to an HPLC system. The solubility data obtained using the automated in-line microfiltration system are in good agreement with the results obtained using conventional manual preparation techniques. Automating this labor-intensive and often variable portion of the solubility determination provides a reliable means of improving both the consistency and quality of solubility data.
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Chemical Development, GlaxoSmithKline Research and Development, Five Moore Drive, Research Triangle Park, NC 27709-3398, USA. dhi17170@gsk.com
Brennan
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Pullen
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D002851:Chromatography, High Pressure Liquid; D005374:Filtration; D012995:Solubility