Cytoplasmic localization is important for transcription factor nuclear factor-kappa B activation by hepatitis C virus core protein through its amino terminal region.
We previously reported that hepatitis C virus core protein (core) activates the transcription factor nuclear factor-kappa B (NF-kappa B) when expressed transiently. In the present study, we investigated the relationship between the NF-kappa B activation capacity and subcellular localization of the core. By changing the subcellular localization of the C-terminally truncated core from the nucleus to the cytoplasm, NF-kappa B was activated. In addition, NF-kappa B activity was augmented by forcing the mutated core to move to the endoplasmic reticulum. It was also suggested that the region from aa 21 to 80 of the core is involved in the activation of NF-kappa B.
Watashi
K
K
Laboratory of Human Tumor Viruses, Department of Viral Oncology, Institute for Virus Research, Kyoto University, Japan.
Hijikata
M
M
Marusawa
H
H
Doi
T
T
Shimotohno
K
K
D016475:3T3 Cells; D000818:Animals; D002460:Cell Line; D003593:Cytoplasm / Q000378:metabolism*; D004721:Endoplasmic Reticulum / Q000378:metabolism; D016174:Hepacivirus / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity; D006526:Hepatitis C / Q000821:virology; D006801:Humans; D051379:Mice; D016328:NF-kappa B / Q000737:chemistry / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics; D015533:Transcriptional Activation; D014162:Transfection; D014758:Viral Core Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
D016328:NF-kappa B; D014758:Viral Core Proteins; C069130:nucleocapsid protein, Hepatitis C virus