title: Fendiline, an anti-anginal drug, increases intracellular Ca2+ in PC3 human prostate cancer cells.
abstract: BACKGROUND
  The effects of the anti-anginal drug fendiline on intracellular Ca2+ concentrations ([Ca2+]i) in human PC3 prostate cancer cells were examined.
  
  
  METHODS
  [Ca2+]i was measured using the fluorescent dye fura-2.
  
  
  RESULTS
  Fendiline (0.5-100 microM) increased [Ca2+]i in a concentration-dependent manner. Ca2+ removal partly inhibited the Ca2+ signals. In Ca2+-free medium, pretreatment with 100 microM fendiline inhibited most of the [Ca2+]i increase induced by 1 microM thapsigargin (an endoplasmic reticulum Ca2+ pump inhibitor), and pretreatment with thapsigargin abolished the fendiline-induced [Ca2+]i increases. Adding 3 mM Ca2+ increased [Ca2+]i in cells pretreated with 0.5-200 microM fendiline in Ca2+-free medium. Pretreatment with 1 microM U73122 to block the formation of inositol-1.4.5-trisphosphate (IP3) did not alter fendiline-induced internal Ca2+ release.
  
  
  CONCLUSIONS
  The anti-anginal drug fendiline induced internal Ca2+ release and external Ca2+ entry. Because prolonged increases in [Ca2+]i may lead to cell injury and death, the long-term effect of fendiline on the function of prostate cancer cells should be investigated.
authors.affiliation: Department of Medical Education and Research, Kaohsiung Veterans General Hospital, Taiwan.
authors.forename: C R
authors.initials: CR
authors.lastname: Jan
authors.forename: K C
authors.initials: KC
authors.lastname: Lee
authors.forename: K J
authors.initials: KJ
authors.lastname: Chou
authors.forename: J S
authors.initials: JS
authors.lastname: Cheng
authors.forename: J L
authors.initials: JL
authors.lastname: Wang
authors.forename: Y K
authors.initials: YK
authors.lastname: Lo
authors.forename: H T
authors.initials: HT
authors.lastname: Chang
authors.forename: K Y
authors.initials: KY
authors.lastname: Tang
authors.forename: C C
authors.initials: CC
authors.lastname: Yu
authors.forename: J K
authors.initials: JK
authors.lastname: Huang
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D005275:Fendiline / Q000494:pharmacology*; D006801:Humans; D015544:Inositol 1,4,5-Trisphosphate / Q000096:biosynthesis; D008297:Male; D011471:Prostatic Neoplasms / Q000378:metabolism* / Q000473:pathology; D014407:Tumor Cells, Cultured; D014665:Vasodilator Agents / Q000494:pharmacology*
chemical_list: D002121:Calcium Channel Blockers; D014665:Vasodilator Agents; D015544:Inositol 1,4,5-Trisphosphate; D000255:Adenosine Triphosphate; D005275:Fendiline; D002118:Calcium