Alcohol and aldehyde dehydrogenase activity in the stomach and small intestine of rats poisoned with methanol.
The activities of alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) were measured with fluorogenic naphthaldehydes in the stomach and small intestine homogenates of rats dosed with 6 g methanol/kg bw after 6, 12, 24 h and 2, 5, 7 days. After intoxication with a sublethal dose, the ADH activity measured with these naphthaldehydes and ALDH activities in the stomach and small intestine were significantly decreased. This inhibition is stronger in the stomach and probably depends on cell damage and protein denaturation. We conclude that the activity measured with 6-methoxy-2-naphthaldehyde (MONAL-62) may be due to the activity of rat ADH-1 isoenzyme, and the activity detected with 4-methoxy-1-naphthaldehyde (MONAL-41) to the activity of rat ADH-2 isoenzyme.
Chrostek
L
L
Department of Biochemical Diagnostics, Medical Academy, Białystok, Poland.
Szczepura
D
D
Szmitkowski
M
M
Jelski
W
W
Wierzchowski
J
J
D000426:Alcohol Dehydrogenase / Q000187:drug effects / Q000378:metabolism*; D000444:Aldehyde Dehydrogenase / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D007421:Intestine, Small / Q000187:drug effects / Q000201:enzymology / Q000473:pathology; D008297:Male; D000432:Methanol / Q000506:poisoning*; D051381:Rats; D017208:Rats, Wistar; D012997:Solvents / Q000506:poisoning*; D013270:Stomach / Q000187:drug effects / Q000201:enzymology / Q000473:pathology
D012997:Solvents; D000426:Alcohol Dehydrogenase; D000444:Aldehyde Dehydrogenase; D000432:Methanol