title: A 9 year study of clarithromycin and metronidazole resistance in Helicobacter pylori from Spanish children.
abstract: The evolution of clarithromycin, metronidazole and amoxycillin resistance in 246 Spanish Helicobacter pylori clinical isolates, obtained from paediatric patients during a 9 year period, was determined by an agar dilution technique. Clarithromycin resistance (MIC 1 mg/L) was 2.27% (IC95 0.05-12.02) in 1991-1993, 20.98% (IC95 12.72-31.46) in 1994-1996 and 28.33% (IC95 20.48-37.28) in 1997-1999 (P < 0.01). Metronidazole resistance (MIC 8 mg/L) was 7.14% (IC95 1.49-19.48) in 1991-1993, 20.25% (IC95 12.04-30.79) in 1994-1996 and 43.90% (IC95 32.95-55.30) in 1997-1999 (P < 0.01). Amoxycillin resistance was not found (all strains showed MICs < 2 mg/L).
authors.affiliation: Department of Microbiology, Hospital Universitario de la Princesa, Diego de León 62, 28006 Madrid, Spain. mlbrea@microb.net
authors.forename: M
authors.initials: M
authors.lastname: López-Brea
authors.forename: M J
authors.initials: MJ
authors.lastname: Martínez
authors.forename: D
authors.initials: D
authors.lastname: Domingo
authors.forename: T
authors.initials: T
authors.lastname: Alarcón
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology* / Q000627:therapeutic use; D016009:Chi-Square Distribution; D002648:Child; D002675:Child, Preschool; D017291:Clarithromycin / Q000494:pharmacology* / Q000627:therapeutic use; D016001:Confidence Intervals; D024881:Drug Resistance, Bacterial; D016481:Helicobacter Infections / Q000188:drug therapy*; D016480:Helicobacter pylori / Q000187:drug effects* / Q000302:isolation & purification; D006801:Humans; D008795:Metronidazole / Q000494:pharmacology* / Q000627:therapeutic use; D008826:Microbial Sensitivity Tests; D013030:Spain
chemical_list: D000900:Anti-Bacterial Agents; D008795:Metronidazole; D017291:Clarithromycin