title: Cytotoxic T lymphocyte responses to vaccinia virus antigens but not HIV-1 subtype E envelope protein seen in HIV-1 seronegative Thais.
abstract: The HIV-1 prime boost phase I/II vaccine trial using a recombinant canarypox vector, vCP1521, containing subtype E env (gp120), and subtype B env (gp41), gag and protease has started in Thailand. We have demonstrated that although 4 from 15 human immunodeficiency virus type 1 (HIV-1) seronegative Individuals showed cytotoxic T lymphocyte (CTL) responses to vaccinia virus antigens, none of them showed specific CTL responses to subtype E Env after in vitro stimulation. This preliminary study suggests that specific CTL responses to subtype E envelope detected in HIV-1 seronegative Individuals after vaccination should be considered as specific responses to the immunization.
authors.affiliation: Department of Microbiology, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok, Thailand.
authors.forename: W
authors.initials: W
authors.lastname: Kantakamalakul
authors.forename: J
authors.initials: J
authors.lastname: Cox
authors.forename: U
authors.initials: U
authors.lastname: Kositanont
authors.forename: S
authors.initials: S
authors.lastname: Siritantikorn
authors.forename: K
authors.initials: K
authors.lastname: Limbach
authors.forename: D
authors.initials: D
authors.lastname: Birx
authors.forename: P
authors.initials: P
authors.lastname: Thongcharoen
authors.forename: P
authors.initials: P
authors.lastname: Puthavathana
mesh_terms: D000328:Adult; D000956:Antigens, Viral / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology; D005260:Female; D015488:HIV Antigens / Q000276:immunology*; D015699:HIV Envelope Protein gp120 / Q000276:immunology*; D018023:HIV Seronegativity / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology*; D004854:Herpesvirus 4, Human / Q000276:immunology; D006801:Humans; D016130:Immunophenotyping; D008297:Male; D008875:Middle Aged; D012016:Reference Values; D012680:Sensitivity and Specificity; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D013785:Thailand; D014616:Vaccinia virus / Q000276:immunology*
chemical_list: D000956:Antigens, Viral; D015488:HIV Antigens; D015699:HIV Envelope Protein gp120