title: A comparative evaluation of three root-end filling materials: an in vitro leakage study using Prevotella nigrescens.
abstract: This study used Prevotella nigrescens to evaluate the sealing ability of Geristore, Super-EBA, and ProRoot when used as root-end filling materials. One hundred single-canal, extracted, human teeth were cleaned and shaped with Gates-Glidden burs and .04 Taper (ProFile Series 29) rotary instruments. The root-ends were resected, and a 3-mm deep root-end preparation was made with ultrasonic tips. After steam sterilization, the teeth were randomly divided into three groups of 30. Each group was root-end-filled with a different material. Five root-end cavities were left open and served as positive controls; another five root-end cavities filled with sticky wax and covered with two layers of nail polish acted as negative controls. The teeth were attached to presterilized snap-on plastic caps; the root-ends submerged into 12-ml vials of chopped meat carbohydrate broth and placed in an anaerobic chamber. Twice a week, a sterile micropipette was used to inoculate 0.1 ml of a broth culture of Prevotella nigrescens into the root canal of each tooth. Results after 47 days indicated there were no significant differences between the three root-end filling materials against penetration of Prevotella nigrescens.
authors.affiliation: University of Detroit Mercy, School of Dentistry, MI 48219-0900, USA.
authors.forename: S Q
authors.initials: SQ
authors.lastname: Scheerer
authors.forename: H R
authors.initials: HR
authors.lastname: Steiman
authors.forename: J
authors.initials: J
authors.lastname: Cohen
mesh_terms: D017607:Aluminum Compounds / Q000737:chemistry / Q000627:therapeutic use; D000704:Analysis of Variance; D001047:Apicoectomy; D017610:Calcium Compounds / Q000737:chemistry / Q000627:therapeutic use; D003763:Dental Leakage / Q000382:microbiology*; D003786:Dental Pulp Cavity / Q000382:microbiology; D017220:Dentin-Bonding Agents / Q000737:chemistry / Q000627:therapeutic use; D004338:Drug Combinations; D005899:Glass Ionomer Cements / Q000737:chemistry / Q000627:therapeutic use; D006171:Gutta-Percha / Q000627:therapeutic use; D006801:Humans; D010087:Oxides / Q000737:chemistry / Q000627:therapeutic use; D018720:Prevotella / Q000145:classification / Q000502:physiology*; D012117:Resins, Synthetic / Q000737:chemistry / Q000627:therapeutic use; D012184:Retrograde Obturation; D012387:Root Canal Filling Materials / Q000737:chemistry / Q000627:therapeutic use*; D018915:Root Canal Preparation; D017640:Silicates / Q000737:chemistry / Q000627:therapeutic use; D018709:Statistics, Nonparametric; D013242:Sterilization / Q000379:methods; D014464:Ultrasonic Therapy
chemical_list: D017607:Aluminum Compounds; D017610:Calcium Compounds; D017220:Dentin-Bonding Agents; D004338:Drug Combinations; C074933:Geristore; D005899:Glass Ionomer Cements; D010087:Oxides; D012117:Resins, Synthetic; D012387:Root Canal Filling Materials; D017640:Silicates; C086631:mineral trioxide aggregate; C084074:Super EBA; D006171:Gutta-Percha