[Effects of composite blood-activating decoction on bone marrow microenvironment in mice of immune-induced aplastic anemia].
OBJECTIVE
To explore the mechanism of elevating efficacy for aplastic anemia (AA) by using blood-activating and stasis-eliminating drugs.


METHODS
Immune-induced aplastic anemia model was established. Each mouse was gastrogavaged by 0.2 ml 100% composite blood-activating decoction (CBAD) twice a day. On the 10th day, the bone marrow histology, CFU-F, adhesive function of the cultured stromal cell layer, bone marrow PO2 were observed.


RESULTS
In CBAD group, the WBC count, bone marrow karyocytes, bone marrow hematopoietic tissue volume, CFU-F count were significantly higher than those in AA group (P < 0.01). Moreover, the adhesive function of stromal cells and bone marrow PO2 recovered to normal level.


CONCLUSIONS
The commonly used composite blood-activating decoction could promote the recovery and oxygen-supply of bone marrow microenvironment in AA mice, and improved the bone marrow hematopoiesis.
Shu
Y
Y
Tongji Hospital, Tongji Medical University, Wuhan 430030.
Sun
H
H
Liu
W
W
D000741:Anemia, Aplastic / Q000188:drug therapy / Q000473:pathology; D000818:Animals; D001853:Bone Marrow / Q000473:pathology*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology / Q000627:therapeutic use; D005260:Female; D006412:Hematopoietic Stem Cells / Q000473:pathology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008811:Mice, Inbred DBA; D011897:Random Allocation
D004365:Drugs, Chinese Herbal