Genetic transformation of Trametes versicolor to phleomycin resistance with the dominant selectable marker shble.
We have developed a stable, DNA-mediated transformation system for the white-rot basidiomycete Trametes versicolor based on the dominant selectable marker shble (phleomycin resistance). We employed a vector containing the selectable marker under control of expression sequences from the basidiomycete Schizophyllum commune and a polyethylene glycol/ CaCl2 protoplast-fusion technique to introduce the transforming DNA. This transformation system generated stable phleomycin-resistant transformants at a frequency of four to seven transformants/microg of transforming DNA.
Bartholomew
K
K
Department of Biological Sciences, Central Connecticut State University, New Britain 06050, USA. bartholomewk@ccsu.edu
Dos Santos
G
G
Dumonceaux
T
T
Charles
T
T
Archibald
F
F
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001487:Basidiomycota / Q000187:drug effects / Q000235:genetics*; D015139:Blotting, Southern; D025141:Drug Resistance, Fungal; D010692:Phleomycins / Q000494:pharmacology*; D014170:Transformation, Genetic
D000900:Anti-Bacterial Agents; D010692:Phleomycins