Method for non-invasively recording electrocardiograms in conscious mice.
BACKGROUND
The rapid increase in the development of mouse models is resulting in a growing demand for non-invasive physiological monitoring of large quantities of mice. Accordingly, we developed a new system for recording electrocardiograms (ECGs) in conscious mice without anesthesia or implants, and created Internet-accessible software for analyzing murine ECG signals. The system includes paw-sized conductive electrodes embedded in a platform configured to record ECGs when 3 single electrodes contact 3 paws.


RESULTS
With this technique we demonstrated significantly reduced heart rate variability in neonates compared to adult mice. We also demonstrated that female mice exhibit significant ECG differences in comparison to age-matched males, both at baseline and in response to beta-adrenergic stimulation.


CONCLUSIONS
The technology we developed enables non-invasive screening of large numbers of mice for ECG changes resulting from genetic, pharmacological, or pathophysiological alterations. Data we obtained non-invasively are not only consistent with what have been reported using invasive and expensive methods, but also demonstrate new findings regarding gender-dependent and age-dependent variations in ECGs in mice.
Chu
V
V
Cardiovascular Division, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA. vchu1@caregroup.harvard.edu
Otero
J M
JM
Lopez
O
O
Morgan
J P
JP
Amende
I
I
Hampton
T G
TG
D000318:Adrenergic beta-Agonists / Q000494:pharmacology; D000818:Animals; D003243:Consciousness; D004562:Electrocardiography / Q000379:methods*; D005260:Female; D006321:Heart / Q000254:growth & development; D006339:Heart Rate / Q000187:drug effects; D007545:Isoproterenol / Q000494:pharmacology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D012737:Sex Factors; D012984:Software; D013045:Species Specificity; D013564:Sympathetic Nervous System / Q000502:physiology
D000318:Adrenergic beta-Agonists; D007545:Isoproterenol