Solution structure and interaction surface of the C-terminal domain from p47: a major p97-cofactor involved in SNARE disassembly.
p47 is the major protein identified in complex with the cytosolic AAA ATPase p97. It functions as an essential cofactor of p97-regulated membrane fusion, which has been suggested to disassemble t-t-SNARE complexes and prepare them for further rounds of membrane fusion. Here, we report the high-resolution NMR structure of the C-terminal domain from p47. It comprises a UBX domain and a 13 residue long structured N-terminal extension. The UBX domain adopts a characteristic ubiquitin fold with a betabetaalphabetabetaalphabeta secondary structure arrangement. Three hydrophobic residues from the N-terminal extension pack closely against a cleft in the UBX domain. We also identify, for the first time, the p97 interaction surface using NMR chemical shift perturbation studies.
Yuan
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X
Department of Biological Sciences, Wolfson Laboratories, Imperial College of Science Technology and Medicine, London, South Kensington, SW7 2AY, UK.
Shaw
A
A
Zhang
X
X
Kondo
H
H
Lally
J
J
Freemont
P S
PS
Matthews
S
S
D000251:Adenosine Triphosphatases / Q000737:chemistry* / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D053487:DEAD-box RNA Helicases; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D009687:Nuclear Proteins / Q000737:chemistry* / Q000378:metabolism*; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D051381:Rats; D050600:SNARE Proteins; D016415:Sequence Alignment; D012996:Solutions; D014452:Ubiquitins / Q000737:chemistry; D033921:Vesicular Transport Proteins
D008565:Membrane Proteins; D009687:Nuclear Proteins; D050600:SNARE Proteins; D012996:Solutions; D014452:Ubiquitins; D033921:Vesicular Transport Proteins; C075446:Ddx39b protein, rat; D000251:Adenosine Triphosphatases; C404942:p97 ATPase; D053487:DEAD-box RNA Helicases