Identification and characterization of Saccharomyces cerevisiae strains isolated from West African sorghum beer.
The occurrence and characterization of yeasts isolated from sorghum beer produced in Ghana and Burkina Faso, West Africa, were investigated. The yeasts involved in the fermentations were found to consist of Saccharomyces spp. almost exclusively. Of the isolates investigated, 45% were identified as Saccharomyces cerevisiae, whereas more than half of the isolates (53%) had physiological properties atypical of S. cerevisiae or any other member of the complex sensu strictu, as they were able to assimilate only glucose, maltose and ethanol as carbon sources. Both ITS-PCR RFLP and PFGE strongly indicated that these isolates were related to S. cerevisiae, regardless of their phenotypic characteristics. Sequencing of the D1/D2 domain of the 26S rDNA confirmed the close relatedness to S. cerevisiae with 0.5% nucleotide differences. The MAL1 and MAL3 loci were found for all isolates as the only recognized MAL loci. Besides, for 40% of the isolates the MAL61 probe hybridized to a position of about 950 kbp, which has not formerly been described as a MAL locus. The results showed that the spontaneous fermentation of West African sorghum beer is dominated by a variety of strains of S.cerevisiae not previously described, among which starter cultures should be selected.
van der Aa Kühle
A
A
Department of Dairy and Food Science, Food Microbiology, The Royal Veterinary and Agricultural University, Rolighedsvej 30, DK-1958 Copenhagen C, Denmark. aak@kvl.dk
Jesperen
L
L
Glover
R L
RL
Diawara
B
B
Jakobsen
M
M
D001483:Base Sequence; D001515:Beer / Q000382:microbiology*; D002050:Burkina Faso; D002352:Carrier Proteins / Q000235:genetics; D015825:Chromosomes, Fungal / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D005285:Fermentation; D005656:Fungal Proteins / Q000235:genetics; D005800:Genes, Fungal; D005838:Genotype; D005869:Ghana; D008320:Maltose / Q000378:metabolism; D008969:Molecular Sequence Data; D009004:Monosaccharide Transport Proteins; D010641:Phenotype; D006109:Poaceae; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D012335:RNA, Ribosomal / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins; D017422:Sequence Analysis, DNA; D027981:Symporters
D002352:Carrier Proteins; D004271:DNA, Fungal; D004275:DNA, Ribosomal; D005656:Fungal Proteins; D009004:Monosaccharide Transport Proteins; D012335:RNA, Ribosomal; C065096:RNA, ribosomal, 26S; D029701:Saccharomyces cerevisiae Proteins; D027981:Symporters; C067575:maltose transport system, S cerevisiae; D008320:Maltose