[Cloning and primary analysis of 3 'end genome of two alphaviruses isolated from Hainan Province of China].
OBJECTIVE
To further characterize the HBbl7 and Ml viruses isolated from Hainan Province by molecular biology.


METHODS
The fragment containing partial El and 3'untranslated region of each of the viral isolate was amplified by RT-PCR, then subcloned. Recombinant was screened and sequenced. Analysis and comparison were carried out.


RESULTS
1.6kb fragment was amplified from HBbl7 and a 1.3kb was from Ml Comparison of sequences showed, in 3'untranslated region the nucleotide homology between HBbl7 and T48, the prototype of Ross River virus, is 99%, between Ml and Sagiyama virus is 98%. In the partial El sequence, the nucleotide (amino acid) homology between HBbl7 and T48 is 99%(99%),between Ml and Sagiyama virus is 97%(99%), and between Ml and Cetah virus is 94% (98%).


CONCLUSIONS
Sequence analysis showed HBb17 virus belongs to Ross River virus, Ml virus belongs to Sagiyama virus or Cetah virus.
Zhao
W
W
Institute of Virology, Chinese Academy of Preventive Medicine, Beijing 100052, China.
Zhou
G
G
He
H
H
D000524:Alphavirus / Q000235:genetics* / Q000302:isolation & purification; D000595:Amino Acid Sequence; D001483:Base Sequence; D002681:China; D003001:Cloning, Molecular; D016679:Genome, Viral; D008969:Molecular Sequence Data; D012398:Ross River virus / Q000235:genetics; D017421:Sequence Analysis; D012689:Sequence Homology, Nucleic Acid