Multiplex polymerase chain reaction for distinguishing Taylorella equigenitalis from Taylorella equigenitalis-like organisms.
It is difficult to distinguish isolates of Taylorella equigenitalis, the cause of contagious equine metritis, from a T. equigenitalis-like organism isolated from asymptomatic donkeys and horses. Although T. equigenitalis is responsible for a severe, contagious disease of the reproductive tract of equids, the T. equigenitalis-like organism, although contagious, does not appear to produce disease. Because of the economic consequences of correctly distinguishing isolates of these 2 microorganisms, a polymerase chain reaction (PCR)-based assay was developed that will distinguish isolates of T. equigenitalis from the T. equigenitalis-like microorganism. The primers used in the PCR assay were designed to amplify unique regions of the gene encoding the 16S ribosomal RNA.
Arata
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Department of Pathology, Microbiology, and Immunology, School of Veterinary Medicine, University of California, Davis 95616, USA.
Cooke
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CL
Jang
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SS
Hirsh
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DC
D000595:Amino Acid Sequence; D000818:Animals; D017931:DNA Primers; D018397:Equidae; D016905:Gram-Negative Bacterial Infections / Q000175:diagnosis / Q000235:genetics / Q000662:veterinary*; D006734:Horse Diseases / Q000175:diagnosis / Q000235:genetics / Q000382:microbiology*; D006736:Horses; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000662:veterinary; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012680:Sensitivity and Specificity; D020625:Taylorella equigenitalis / Q000235:genetics* / Q000302:isolation & purification
D017931:DNA Primers; D012336:RNA, Ribosomal, 16S