Biostoning of denims by Penicillium occitanis (Pol6) cellulases.
The Pol6 mutant of Penicillium occitanis, secreting a large quantity of cellulases, was cultivated in fermentor using a local paper pulp as an inducer substrate. A high titer of extracellular cellulase activity was reached after a fed batch process: 23 IU x ml(-1) filter paper activity, 21 IU x ml(-1) CMCases activity (endoglucanase units) and 25 mg x ml(-1) of proteins. Various tests were done to compare the action of the P. occitanis cellulases with those commercially available and with the traditional stonewashing process. This cellulase preparation was successfully applied in a biostoning process at an industrial scale. The abrasive effect of the P. occitanis cellulases was very uniform and with an efficiency comparable to that obtained by the commercial ones.
Belghith
H
H
Laboratory Génétique Moléculaire des Eucaryotes, Centre of Biotechnologie, Sfax BP K, 3038, Sfax, Tunisia. hafeth.belghith@cbs.rnrt.tn
Ellouz-Chaabouni
S
S
Gargouri
A
A
D002480:Cellulase / Q000737:chemistry*; D010407:Penicillium / Q000201:enzymology*; D013784:Textiles
D002480:Cellulase