Vanin genes are clustered (human 6q22-24 and mouse 10A2B1) and encode isoforms of pantetheinase ectoenzymes.
The mouse Vanin-1 molecule plays a role in thymic reconstitution following damage by irradiation. We recently demonstrated that it is a membrane pantetheinase (EC 3.56.1.-). This molecule is the prototypic member of a larger Vanin family encoded by at least two mouse (Vanin-1 and Vanin-3) and three human (VNN1, VNN2, VNN3) orthologous genes. We now report (1) the structural characterization of the human and mouse Vanin genes and their organization in clusters on the 6q22-24 and 10A2B1 chromosomes, respectively; (2) identification of the human VNN3 gene and the demonstration that the mouse Vanin-3 molecule is secreted by cells, and (3) that the Vanin genes encode different isoforms of the mammalian pantetheinase activity. Thus, the Vanin family represents a novel class of secreted or membrane-associated ectoenzymes. We discuss here their possible role in processes pertaining to tissue repair in the context of oxidative stress.
Martin
F
F
Centre d'Immunologie de Marseille-Luminy, INSERM-CNRS-Université de la Méditerranée, France.
Malergue
F
F
Pitari
G
G
Philippe
J M
JM
Philips
S
S
Chabret
C
C
Granjeaud
S
S
Mattei
M G
MG
Mungall
A J
AJ
Naquet
P
P
Galland
F
F
D000581:Amidohydrolases / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015815:Cell Adhesion Molecules / Q000235:genetics*; D002896:Chromosomes, Human, Pair 6 / Q000235:genetics*; D017124:Conserved Sequence; D058851:GPI-Linked Proteins; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family; D020033:Protein Isoforms / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution
D015815:Cell Adhesion Molecules; D058851:GPI-Linked Proteins; D008565:Membrane Proteins; D020033:Protein Isoforms; D000581:Amidohydrolases; C022314:pantetheinase