Comparison of porcine reproductive and respiratory syndrome virus growth in media supplemented with fetal bovine serum or a serum replacement.
Commercially available serum replacements are often used in cell culture as a cheaper and less variable substitute for fetal bovine serum (FBS). The growth of porcine reproductive and respiratory syndrome virus (PRRSV) isolates in CRL11171 cells maintained in a medium supplemented with FBS was compared with virus propagation in the same cell line maintained in the same medium with a serum replacement. The PRRSV replicated significantly better when the cell culture medium was supplemented with FBS. The results of this study have implications for the use of serum replacement-supplemented medium for PRRSV diagnosis by virus isolation.
Schommer
S K
SK
Department of Veterinary Microbiology and Preventive Medicine, Veterinary Medical Research Institute, College of Veterinary Medicine, Iowa State University, Ames 50011, USA.
Carpenter
S L
SL
Paul
P S
PS
D000818:Animals; D002417:Cattle; D018929:Cell Culture Techniques / Q000379:methods; D003470:Culture Media; D005312:Fetal Blood; D019316:Porcine respiratory and reproductive syndrome virus / Q000302:isolation & purification / Q000472:pathogenicity / Q000502:physiology*; D013048:Specimen Handling
D003470:Culture Media