title: Immunosuppressive effects of rubidatum in mice.
abstract: OBJECTIVE
  To study the effect of rubidatum (Rub) on immune function in normal mice.
  
  
  METHODS
  Serum lysozyme concentration (SLC) was measured using micrococcus lysodiekticus as a substrate. Delayed type hypersensitivity (DTH) was determined by measuring the thickness of the right hind footpad 24 h after the injection of 1 x 10(8) washed sRBC (50 microL 10% sRBC). Serum hemolysin concentration was determined by OD measuring at A540 after the serum was treated with 2-mercaptoethanol. Phagocytic function of peripheral leukocyte (Leu) were determined by the incorporated radioactivity of [3H]TdR. The hemolytic activity of plaque forming cell (PFC) was determined by measuring the lymphocytemediated hemolysis of sheep red blood cell in vitro. T- and B-lymphocyte transformation (TLT and BLT) were induced by phytohemagglutinin (PHA) and lipopolysacharide (LPS) respectively and measured by the incorporation of [3H]TdR.
  
  
  RESULTS
  Rub 125, 500, 2000 mg.kg-1.d-1 p.o. to BALB/c (or NIH) mice decreased the SLC; inhibited the phagocytosing functions of peripheral leukocytes; diminished the hemolytic activity of PFC; decreased the HC50; inhibited the DTH reaction; and showed inhibitory effects on TLT and BLT.
  
  
  CONCLUSIONS
  Rub has immunosuppressive effects on immune system in mice by affecting M phi, T, and B lymphocyte, which suggests that Rub has inhibitory effects on both nonspecific and specific immune function.
authors.affiliation: Department of Internal Medicine, 474th Hospital, PLA, Urumqi 830011, China.
authors.forename: S L
authors.initials: SL
authors.lastname: Yang
authors.forename: F
authors.initials: F
authors.lastname: Liu
authors.forename: H
authors.initials: H
authors.lastname: Zhang
authors.forename: Q L
authors.initials: QL
authors.lastname: Dou
mesh_terms: D000818:Animals; D005260:Female; D006462:Hemolytic Plaque Technique; D006968:Hypersensitivity, Delayed; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D007962:Leukocytes / Q000502:physiology; D008213:Lymphocyte Activation / Q000187:drug effects; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009113:Muramidase / Q000097:blood; D009284:Naphthols / Q000494:pharmacology*; D010587:Phagocytosis / Q000187:drug effects
chemical_list: D007166:Immunosuppressive Agents; D009284:Naphthols; C048580:rubidate; D009113:Muramidase