title: Multiple protein differences distinguish clam leukemia cells from normal hemocytes: evidence for the involvement of p53 homologues.
abstract: In coastal locations, marine invertebrates, primarily molluscs, develop fatal leukemias in their blood or hemolymph. In the clam Mya arenaria, non-adhesive, mitotic, spherical leukemia cells replace adhesive, motile, normal hemocytes as leukemia progresses. End-stage leukemia cells express a unique antigen, IE10, while normal cells express the 2A4 marker. The goals of this work were to further differentiate the normal and leukemia specific antigens relative to protein structure, determine if other protein distinctions exist, and examine p53 gene family expression in both cell types. Recognized by the monoclonal antibody 2A4, normal cells express a 185-kDa glycoprotein that may have multiple forms. Detected by the monoclonal antibody 1E10, leukemic cells express a very hydrophobic 252-kDa glycoprotein that is likely to be a transmembrane protein with spectrin/dystrophin-like characteristics. After normalization to the major cytoskeletal protein actin, sodium dodecyl sulfate-polyacrylamide gel electrophoresis reveals major distinguishing protein and glycoprotein differences between the two cell types. Most obvious is the near-absence of tubulin in the non-mitotic normal hemocytes. We have also characterized the expression of p53 gene family members in normal and end-stage leukemia cells, finding shifts in expression of the p53 gene homologues p73 and p97 coincident with leukemia-specific protein synthesis.
authors.affiliation: Department of Physiology and Biophysics, Boston University School of Medicine, Boston, MA 02118, USA. rstephen@bu.edu
authors.forename: R E
authors.initials: RE
authors.lastname: Stephens
authors.forename: C W
authors.initials: CW
authors.lastname: Walker
authors.forename: C L
authors.initials: CL
authors.lastname: Reinisch
mesh_terms: D000818:Animals; D000951:Antigens, Neoplasm / Q000032:analysis; D049872:Bivalvia; D003598:Cytoskeletal Proteins; D004591:Electrophoresis, Polyacrylamide Gel; D015972:Gene Expression Regulation, Neoplastic; D016158:Genes, p53 / Q000235:genetics*; D006434:Hemocytes / Q000502:physiology*; D007938:Leukemia / Q000235:genetics* / Q000662:veterinary*; D014404:Tubulin / Q000032:analysis; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis
chemical_list: D000951:Antigens, Neoplasm; D003598:Cytoskeletal Proteins; D014404:Tubulin; D016159:Tumor Suppressor Protein p53