title: Trichinella spp. in ostrich meat: a public health risk?
abstract: In the present work the biological behaviour of T. spiralis and T. pseudospiralis in ostriches is reported. Oral infections were performed in eight ostriches with two infective doses (10,000 and 80,000 larvae) for each species of Trichinella. On day 0, 30 and 60 p.i. blood samples were collected to assay the serum changes concerning specific muscle enzyme activities and total proteins. The immunological study, to determine specific IgG in sera, was conducted employing a monoclonal blocking ELISA. From the carcasses of sacrificed animals, samples of various muscle tissues were examined by the digestion method and by standard histopathologic procedures. The study showed a low susceptibility of the ostriches to T. pseudospiralis; preferential sites of larval distribution were muscle tissues of the legs. T. spiralis could be found in muscle tissues only when a high number of larvae were inoculated. Immunological reactivity was found only in animals infected with higher doses of T. pseudospiralis.
authors.affiliation: Dipartimento di Scienze Biopatologiche Veterinarie Sez. Parassitologia, Università di Perugia, S. Costanzo no. 4, 06100 Perugia, Italy. rprof2f2@unipg.it
authors.forename: D
authors.initials: D
authors.lastname: Piergili Fioretti
authors.forename: A
authors.initials: A
authors.lastname: Moretti
authors.forename: C
authors.initials: C
authors.lastname: Marini
authors.forename: V
authors.initials: V
authors.lastname: Grelloni
authors.forename: M T
authors.initials: MT
authors.lastname: Antognoni
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D001715:Bird Diseases / Q000175:diagnosis / Q000276:immunology / Q000469:parasitology*; D005243:Feces / Q000469:parasitology; D006801:Humans; D007558:Italy; D007814:Larva; D008460:Meat / Q000469:parasitology*; D018482:Muscle, Skeletal / Q000469:parasitology; D011634:Public Health; D020310:Struthioniformes / Q000469:parasitology*; D014234:Trichinella / Q000254:growth & development* / Q000302:isolation & purification; D017160:Trichinella spiralis / Q000254:growth & development* / Q000302:isolation & purification; D014235:Trichinellosis / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*
chemical_list: D000909:Antibodies, Helminth