Determination of amlodipine in human plasma by high-performance liquid chromatography with fluorescence detection.
A sensitive and specific HPLC method has been developed for the assay of amlodipine in human plasma. The assay involves derivatization with 4-chloro-7-nitrobenzofurazan (NBD-Cl), solid-phase extraction on a silica column and isocratic reversed-phase chromatography with fluorescence detection. Nortriptyline hydrochloride was used as an internal standard. The assay was linear over the concentration range of 0.25-18.00 ng/ml. Both of the within-day and day-to-day reproducibility and accuracy were less than 11.80% and 12.00%, respectively. The plasma profile following a single administration of 10 mg amlodipine to a healthy volunteer was presented.
Tatar
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University of Istanbul, Faculty of Pharmacy, Department of Analytical Chemistry, Turkey.
Atmaca
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D017311:Amlodipine / Q000097:blood*; D002121:Calcium Channel Blockers / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence
D002121:Calcium Channel Blockers; D017311:Amlodipine