Investigation of arsenic speciation in oyster test reference material by multidimensional HPLC-ICP-MS and electrospray tandem mass spectrometry (ES-MS-MS).
Multidimensional (size-exclusion-anion-exchange-cation-exchange) liquid chromatography with ICP-MS detection was developed to produce a map of water-soluble species in an oyster test reference material. The presence of arsenobetaine, trimethyl(2-carboxyethyl)arsonium inner salt, arsenocholine, dimethylarsonic acid, tetramethylammonium ion, As(v) and two arsenosugars was demonstrated by ES-MS-MS. A previously unreported compound was isolated and identified by ES-MS-MS as 5-dimethylarsinoyl-beta-ribofuranose. Anion-exchange chromatography was optimized to produce a chromatographically pure peak of arsenobetaine (accounting for ca. 64% of all water-soluble As present) that was used to quantify this compound.
McSheehy
S
S
CNRS UMR 5034, Hélioparc, Pau-Pyrénées, France.
Pohl
P
P
Lobiński
R
R
Szpunar
J
J
D000818:Animals; D001151:Arsenic / Q000032:analysis / Q000737:chemistry*; D001152:Arsenicals / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D013058:Mass Spectrometry; D010125:Ostreidae / Q000737:chemistry*; D012758:Shellfish / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization
D001152:Arsenicals; D001151:Arsenic