A phenylarsine oxide-binding protein of neutrophil cytosol, which belongs to the S100 family, potentiates NADPH oxidase activation.
By photoaffinity labeling with a tritiated azido derivative of phenylarsine oxide (PAO), 4[N-(4-azido-2-nitrophenyl)amino-[(3)H]acetamido]phenylarsine oxide ([(3)H]azidoPAO), we demonstrate that PAO binds selectively to the S100 A8/A9 complex of bovine neutrophil cytosol (previously known as p7/p23, homologous to the MRP-8/MRP-14 complex of human phagocytes). Using a semirecombinant cell free assay of oxidase activation and the determination of oxidase activity by the production of the superoxide anion O(-)(2), we found that the PAO binding protein (p7/p23) was able to potentiate the activation of NADH oxidase and that this effect was synergized by PAO. The p7/p23 protein complex of bovine neutrophils can therefore be considered as a positive regulator of NADPH oxidase activation in neutrophils.
Doussière
J
J
Département de Biologie Moléculaire et Structurale, Laboratoire de Biochimie et Biophysique des Systèmes Intégrés (UMR CNRS/CEA/UJF n degrees 5092), CEA-Grenoble, 17 Avenue des Martyrs, Grenoble cedex 9, 38054, France. jdoussiere@cea.fr
Bouzidi
F
F
Vignais
P V
PV
D000818:Animals; D000943:Antigens, Differentiation / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D001152:Arsenicals / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D001386:Azides / Q000737:chemistry / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D040501:Calgranulin A; D040502:Calgranulin B; D002417:Cattle; D002474:Cell-Free System / Q000378:metabolism; D002846:Chromatography, Affinity; D003600:Cytosol / Q000737:chemistry / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation / Q000187:drug effects; D046911:Macromolecular Substances; D019255:NADPH Oxidases / Q000378:metabolism*; D018375:Neutrophil Activation / Q000187:drug effects; D009504:Neutrophils / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D019920:Photoaffinity Labels / Q000737:chemistry; D011485:Protein Binding; D009418:S100 Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D013481:Superoxides / Q000032:analysis / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
C110916:4-(N-(4-azido-2-nitrophenyl)aminoacetamido)phenylarsine oxide; D000943:Antigens, Differentiation; D001152:Arsenicals; D001386:Azides; D002135:Calcium-Binding Proteins; D040501:Calgranulin A; D040502:Calgranulin B; D046911:Macromolecular Substances; D019920:Photoaffinity Labels; D009418:S100 Proteins; C029341:oxophenylarsine; D013481:Superoxides; D019255:NADPH Oxidases; D013755:Tetradecanoylphorbol Acetate