[Synergistic effect of the expression of p16INK4 gene and retinoblastoma gene in lung cancer].
In order to clarify the frequency of p16INK4 gene inactivation and its relationship with retinoblastoma (Rb) gene expression in non-small cell lung cancer, in situ double hybridization was established with Bio-labelled Rb cDNA probe and Dig-lablled p16INK4 cDNA probe, and immunohistochemistry was carried out with polyclone rabbit antibodies to human p16INK4 and Rb protein. The results showed that positive hybridization of p16INK4 mRNA showed blue with the detecting system of alkiline phosphatase NBT/BCIP, positive hybridization of Rb mRNA showed red with the detecting system of HPR-AEC. The negative ratios of p16INK4 and Rb gene transcription were 22.6%(7/31) and 16.1%(5/31). The results of immunohistochemistry showed that the negative ratio of p16INK4 protein was 42%(13/31) with p16INK4 polyclone antibody, the negative ratio of pRb was 19.4%(6/31) with polyclone antibody. The data indicated that p16INK4 gene and Rb gene are synergistic in the progression of lung cancer, p16INK4 gene plays a significant role in the development of non-small cell lung cancer.
Cao
X R
XR
College of Life Science, Nanjing Normal University, Nanjing 210097, China.
Zhang
X R
XR
Su
C Q
CQ
D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000032:analysis; D016161:Genes, Retinoblastoma; D019942:Genes, p16; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008175:Lung Neoplasms / Q000737:chemistry / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis
D019941:Cyclin-Dependent Kinase Inhibitor p16; D012333:RNA, Messenger