Quantification of sodium dodecyl sulfate in microliter-volume biochemical samples by visible light spectroscopy.
A method for sodium dodecyl sulfate (SDS) quantitation in microliter-volume complex biochemical samples is described. The quantitation is based on the use of a dye, stains-all, the color of which changes from intense fuchsia to yellow upon addition of SDS. We show that this color change is gradual and proportional to the amount of SDS added to the stains-all solution, thus allowing its use to reliably quantitate SDS in biochemical samples by means of a visible light spectrophotometer. A large number of compounds widely used in biochemistry are herein shown not to interfere with the SDS measurement when they are present in the sample at usual biochemical concentrations. Furthermore, linearity between the color change and the amount of SDS present in the sample is never impaired when huge amounts of these compounds are also present, thus making this quantitation method highly reliable with use of a calibration curve. The method allows easy and reliable quantitation of microgram amounts of SDS in microliter-volume biochemical samples.
Rusconi
F
F
Institut Européen de Chimie et Biologie, ENSCPB, Avenue Pey Berland, Talence Cedex, F-33402, France.f.rusconi@lptc.u-bordeaux.fr
Valton
E
E
Nguyen
R
R
Dufourc
E
E
D000818:Animals; D002232:Carbocyanines; D002417:Cattle; D003124:Colorimetry / Q000379:methods; D004396:Coloring Agents / Q000032:analysis; D004247:DNA; D008027:Light; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine; D012967:Sodium Dodecyl Sulfate / Q000032:analysis*; D012995:Solubility; D012996:Solutions / Q000737:chemistry; D013053:Spectrophotometry / Q000379:methods*; D014867:Water / Q000378:metabolism
D002232:Carbocyanines; D004396:Coloring Agents; D012996:Solutions; D014867:Water; D012710:Serum Albumin, Bovine; D012967:Sodium Dodecyl Sulfate; D004247:DNA; C427203:calf thymus DNA