title: Transient changes of brain-derived neurotrophic factor (BDNF) mRNA expression in hippocampus during moderate ischemia induced by chronic bilateral common carotid artery occlusions in the rat.
abstract: Chronic bilateral common carotid artery occlusion (BCCAO) induces moderate ischemia (oligemia) in the rat forebrain in the absence of overt neuronal damage. In situ hybridization for brain-derived neurotrophic factor (BDNF) mRNA was used to search for a molecular response to moderate ischemia. BDNF mRNA was significantly increased in the hippocampal granule cells at 6 h of occlusion (ANOVA, Tukey test P<0.05). At 1, 7 and 14 days BDNF mRNA levels returned to control levels. The frequency of BDNF gene expression at 6 h was 83%, which was significantly higher than the 7% incidence of histological injury in the hippocampus (Fisher's exact test, P<0.002). Cerebral blood flow was reduced to 75% of control levels in the hippocampus after 1 week of BCCAO when measured with the autoradiographic method. Measurements of tissue flow with a microprobe for laser Doppler flow excluded decreases into the ischemic range during the period when elevated gene expression was observed. Prolonged moderate ischemia (oligemia) is a sufficient stimulus for BDNF gene expression in the hippocampus. These molecular studies provide direct evidence for an involvement of the hippocampus in the BCCAO model.
authors.affiliation: Cerebral Vascular Disease Research Center, Department of Neurology, University of Miami School of Medicine, PO Box 016960, Miami, FL 33101, USA. Rainald@stroke.med.miami.edu
authors.forename: R
authors.initials: R
authors.lastname: Schmidt-Kastner
authors.forename: J
authors.initials: J
authors.lastname: Truettner
authors.forename: B
authors.initials: B
authors.lastname: Lin
authors.forename: W
authors.initials: W
authors.lastname: Zhao
authors.forename: I
authors.initials: I
authors.lastname: Saul
authors.forename: R
authors.initials: R
authors.lastname: Busto
authors.forename: M D
authors.initials: MD
authors.lastname: Ginsberg
mesh_terms: D000818:Animals; D001157:Arterial Occlusive Diseases / Q000150:complications*; D002545:Brain Ischemia / Q000209:etiology / Q000235:genetics / Q000378:metabolism*; D019208:Brain-Derived Neurotrophic Factor / Q000096:biosynthesis* / Q000235:genetics; D017536:Carotid Artery, Common; D002560:Cerebrovascular Circulation; D020869:Gene Expression Profiling; D006624:Hippocampus / Q000098:blood supply / Q000378:metabolism* / Q000473:pathology; D017403:In Situ Hybridization; D008026:Ligation; D008297:Male; D009474:Neurons / Q000378:metabolism / Q000473:pathology; D018384:Oxidative Stress; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D012333:RNA, Messenger