title: Rat auxiliary liver transplantation without portal vein reconstruction: comparison with the portal vein-arterialized model.
abstract: Auxiliary liver transplantation (ALT) has been reintroduced in clinical cases recently and is now believed to be a viable alternative to orthotopic liver transplantation. To provide a simple rat ALT model for studying the physiological and immunological aspects of the ALT graft, a new ALT was performed, and the comparison between this new model and the portal arterialized one that was reported by other investigators was carried out. At first, we confirmed that liver could tolerate the deprivation of its portal flow well, using a portosystemic shunted rat model. The new rat ALT model, in which the ALT graft obtained its blood inflow only from the hepatic artery, was then performed. Our results demonstrated that 50% of the hepatic artery-alone ALT graft showed almost normal structure histologically at 1 month after grafting, with bile secretion preserved. By contrast, only 8% 1-month graft survival was noted in the portal arterialized group, and all grafts stopped bile secretion 1 week after operation. In conclusion, with arterial blood supply alone, the ALT graft survived and demonstrated normal bile secretion function for more than 1 month. Portal vein arterialization is not an appropriate way to establish the graft's blood supply if no pressure adjustment measures were taken in advance.
authors.affiliation: Department of Surgery, Jichi Medical School, Tochigi, Japan.
authors.forename: D
authors.initials: D
authors.lastname: Rong Xiu
authors.forename: S
authors.initials: S
authors.lastname: Hishikawa
authors.forename: M
authors.initials: M
authors.lastname: Sato
authors.forename: H
authors.initials: H
authors.lastname: Nagai
authors.forename: H
authors.initials: H
authors.lastname: Uchida
authors.forename: E
authors.initials: E
authors.lastname: Kobayashi
mesh_terms: D000818:Animals; D001163:Arteriovenous Anastomosis; D004195:Disease Models, Animal; D005260:Female; D006084:Graft Rejection; D006085:Graft Survival; D006499:Hepatic Artery / Q000601:surgery*; D008099:Liver / Q000098:blood supply*; D016031:Liver Transplantation / Q000379:methods*; D008297:Male; D008866:Microsurgery / Q000379:methods*; D011169:Portal Vein / Q000601:surgery*; D051381:Rats; D011917:Rats, Inbred Lew; D012077:Renal Artery / Q000601:surgery*; D012680:Sensitivity and Specificity