title: Use of ultrafiltration probes in sheep to collect interstitial fluid for measurement of calcium and magnesium.
abstract: Studies of calcium and magnesium changes in living animals usually involve blood, urine, and fecal samples. These samples provide only information on whole-body averages and give no indication of differences between tissues. Ultrafiltration probes were developed to sample interstitial fluid from muscle, bone, and subcutaneous tissue of sheep to provide a tool for investigating tissue differences in calcium and magnesium concentrations. The potential of the probes for mineral distribution studies was demonstrated in sheep by using infusion of a calcium gluconate solution.
authors.affiliation: Bioanalytical Systems, Inc., W. Lafayette, IN 47906, USA.
authors.forename: E M
authors.initials: EM
authors.lastname: Janle
authors.forename: J E
authors.initials: JE
authors.lastname: Sojka
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000737:chemistry; D002118:Calcium / Q000032:analysis*; D005110:Extracellular Space / Q000737:chemistry*; D005374:Filtration / Q000295:instrumentation; D008274:Magnesium / Q000032:analysis*; D018482:Muscle, Skeletal / Q000737:chemistry; D012756:Sheep; D013048:Specimen Handling
chemical_list: D008274:Magnesium; D002118:Calcium