title: Effect of oxygen delignification on the rate and extent of enzymatic hydrolysis of lignocellulosic material.
abstract: In this study, we examined the effect of oxygen delignification on the rate and extent of enzymatic hydrolysis (using commercial cellulase and beta-glucosidase) of a number of lignocellulosic substrates, including kraft pulp (model substrate), pulp mill primary clarifier sludge (PCS) and steam-exploded Douglas fir chips. Oxygen delignification removed up to 67% of the lignin from softwood pulp and improved the rate of, and yield from, hydrolysis by up to 111% and 174%, respectively. Glucose yield varied linearly with fractional lignin removal. Oxygen delignification of primary clarifier sludge improved hydrolysis yield by up to 90%. However steam-exploded Douglas fir was very resistant to hydrolysis at low enzyme loading, and oxygen delignification decreased hydrolysis rate and yield.
authors.affiliation: Department of Chemical and Biological Engineering, UBC Pulp and Paper Centre, University of British Columbia, Vancouver, Canada.
authors.forename: K M
authors.initials: KM
authors.lastname: Draude
authors.forename: C B
authors.initials: CB
authors.lastname: Kurniawan
authors.forename: S J
authors.initials: SJ
authors.lastname: Duff
mesh_terms: D001274:Atmospheric Pressure; D002480:Cellulase / Q000378:metabolism*; D002482:Cellulose / Q000032:analysis*; D005947:Glucose / Q000378:metabolism; D006358:Hot Temperature; D006868:Hydrolysis; D007220:Industrial Waste / Q000032:analysis; D007700:Kinetics; D008031:Lignin / Q000032:analysis* / Q000378:metabolism*; D010100:Oxygen / Q000737:chemistry*; D028224:Pseudotsuga / Q000737:chemistry; D013227:Steam; D013997:Time Factors; D014934:Wood; D001617:beta-Glucosidase / Q000378:metabolism*
chemical_list: D007220:Industrial Waste; D013227:Steam; C036909:lignocellulose; C076151:Kraft lignin; D002482:Cellulose; D008031:Lignin; D001617:beta-Glucosidase; D002480:Cellulase; D005947:Glucose; D010100:Oxygen