Effects of colloidal resuscitation fluids on the neutrophil respiratory burst.
BACKGROUND
Experimental studies have revealed that gelatin and HES produce increased neutrophil respiratory burst activity. It was investigated whether 3-percent gelatin (MW 35,000) and three types of 6-percent HES (MW 70,000; degree of substitution, 0.5; 200,000/0.5; 450,000/0.7) preparations can influence superoxide anion production during respiratory burst under clinical conditions.


METHODS
Blood samples were obtained from 40 patients before and 1 hour after the infusion, before anesthesia and surgical treatment. After stimulation with bacteria (Escherichia coli), the respiratory burst was measured by oxidation of nonfluorescent dihydrorhodamine 123 to the fluorescent rhodamine 123 by the use of flow cytometry.


RESULTS
Respiratory burst activity decreased significantly (p = 0.004) from the baseline (60.0 +/- 6.5%) to 1 hour after the administration of the low-molecular-weight HES preparation (55.0 +/- 6.8%). No significant differences in respiratory burst activity could be found after the administration of gelatin or medium-molecular-weight or high- molecular-weight HES solution.


CONCLUSIONS
The investigated administration of gelatin and medium- and high-molecular-weight HES preparations did not influence respiratory burst activity under clinical conditions. However, the neutrophil respiratory burst was impaired after the administration of low-molecular-weight HES. Neutrophil respiratory burst activity may vary according to the type of colloidal plasma substitutes administered.
Jaeger
K
K
Department of Anesthesiology and Intensive Care Medicine, Hannover Medical School, Hannover, Germany. jaeger.karsten@mh-hannover.de
Heine
J
J
Ruschulte
H
H
Jüttner
B
B
Scheinichen
D
D
Kuse
E R
ER
Piepenbrock
S
S
D000328:Adult; D000368:Aged; D003102:Colloids / Q000494:pharmacology*; D005260:Female; D005434:Flow Cytometry; D005780:Gelatin / Q000008:administration & dosage / Q000494:pharmacology; D006801:Humans; D006895:Hydroxyethyl Starch Derivatives / Q000008:administration & dosage / Q000494:pharmacology; D008297:Male; D008875:Middle Aged; D018375:Neutrophil Activation / Q000187:drug effects; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D016897:Respiratory Burst / Q000187:drug effects*; D012151:Resuscitation / Q000379:methods*; D013520:Urologic Surgical Procedures
D003102:Colloids; D006895:Hydroxyethyl Starch Derivatives; D005780:Gelatin