Intact lysosome transport and phagosome function despite kinectin deficiency.
The mechanism of cargo coupling to kinesin motor proteins is a fundamental issue in organelle transport along microtubules. Kinectin has been postulated to function as a membrane anchor protein that attaches various organelles to the prototype motor protein kinesin. To verify the biological relevance of kinectin in vivo, the murine kinectin gene was disrupted by homologous recombination. Unexpectedly, kinectin-deficient mice were viable and fertile, and no gross abnormalities were observed up to 1 year of age. The assembly of the endoplasmic reticulum was essentially unaffected in kinectin-deficient cells. Mitochondria appeared to be correctly distributed throughout the cytoplasm along the microtubules. Furthermore, the stationary distribution and the bidirectional movement of lysosomes did not depend on kinectin. Kinectin-deficient phagocytes internalized and cleared bacteria, indicating that phagosome trafficking and maturation are functional without kinectin. Thus, these data unequivocally indicate that kinectin is not essential for trafficking of lysosomes, phagosomes, and mitochondria in vivo.
Plitz
T
T
Institute of Medical Microbiology, Immunology, and Hygiene, Technical University of Munich, Trogerstrasse 9, D-81675 Munich, Germany.
Pfeffer
K
K
D000483:Alleles; D000818:Animals; D001692:Biological Transport; D001798:Blood Proteins / Q000235:genetics / Q000502:physiology*; D002460:Cell Line; D006801:Humans; D007425:Intracellular Membranes / Q000378:metabolism; D008247:Lysosomes / Q000378:metabolism*; D008565:Membrane Proteins; D051379:Mice; D018345:Mice, Knockout; D016296:Mutagenesis; D015388:Organelles / Q000378:metabolism; D010587:Phagocytosis; D010588:Phagosomes / Q000378:metabolism / Q000502:physiology*; D013347:Subcellular Fractions
D001798:Blood Proteins; C088547:KTN1 protein, human; C511012:Ktn1 protein, mouse; D008565:Membrane Proteins