The usefulness of antioxidative enzymes for the estimation of synthetic effects of PGE1 analogue.
The aim of our research was estimation of the activity of superoxide dysmutase (SOD) and glutathione peroxidase (GPx) in rabbits' blood after administration of single dose of carbon tetrachloride (CCl4) and synthetic analogue PGE1 - Cytotec. The research was carried out on 56 Chinchilla rabbits. By means of stomach tube rabbits were administered respectively: CCl4, Cytotec, CCl4 with Cytotec, indomethacin, Cytotec with indomethacin and CCl4 with Cytotec and indomethacin simultneously. In comparison with control group almost double increase of SOD activity was stated at rabbits which were administered CCl4 and CCl4 with indomethacin. At rabbits which were administered CCl4 with Cytotec and CCl4 with Cytotec and with indomethacin, the SOD activity increased by only 50% in comparison with control group. At rabbits in whose blood the SOD activity increased, the activity of GPx decreased on average by respectively 40% and 15%. The stated changes of SOD and GPx activity in rabbits' blood which were administered prostaglandin apart from CCl4 indicates prostaglandin's indirect interference into hepatocytes antioxidative system and thus its hepatoprotective activity.
Pawlowska-Góral
K
K
General and Analytical Chemistry Institute of Silesian Academy of Medicine, Sosnowiec, Poland. chemogan@infomed.slam.katowice.pl
Wardas
M
M
Maciejewska-Paszek
I
I
Skiba
M
M
Nogaj
S
S
D000818:Animals; D000975:Antioxidants / Q000032:analysis*; D002251:Carbon Tetrachloride / Q000633:toxicity; D056486:Chemical and Drug Induced Liver Injury / Q000097:blood* / Q000201:enzymology; D004338:Drug Combinations; D005979:Glutathione Peroxidase / Q000097:blood*; D006454:Hemoglobins / Q000032:analysis; D007213:Indomethacin / Q000494:pharmacology; D008297:Male; D016595:Misoprostol / Q000494:pharmacology; D011817:Rabbits; D013482:Superoxide Dismutase / Q000097:blood*
D000975:Antioxidants; D004338:Drug Combinations; D006454:Hemoglobins; D016595:Misoprostol; D002251:Carbon Tetrachloride; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D007213:Indomethacin