[The immortalized cell lines induced by human papillomavirus type 16 (HPVS 16) YY1 binding-site mutants have some characteristics of transformed cells].
OBJECTIVE
To study the biological characteristics of the immortalized cell lines of human keratinocytes from foreskin induced by human papillomavirus type 16 (HPV 16) YY1 binding-site mutants.


METHODS
The cellular extracts of immortalized cell lines were prepared and the cellular endogenous p53 proteins were determined with Western blot. The cellular telomerase activities were analyzed with TRAP. The immortalized cells were incubated in a soft agarose medium containing 10% FCS and the anchorage-independent growth abilities of the tested cell lines were evaluated.


RESULTS
Western blot showed that the endogenous p53 protein in all 4 tested clones were undetectable. The telomerases were clearly identified within the 4 tested cell lines, and showed an increasing activity along with the passages of the cells. All the clones were unable to grow in the soft-agarose medium during the earlier passages, but three of them showed anchorage-independent-growth at the passages of 30-35.


CONCLUSIONS
The immortalized cell lines induced by the HPV 16 YY1 binding-site mutants have active telomerase activity and ability of anchorage-independent growth.
Liu
H
H
Beijing Stomatological Hospital, The Capital University of Medical Sciences, Beijing 100050, China.
Dong
X
X
Zhou
W
W
D000362:Agar; D002460:Cell Line; D017077:Culture Media, Conditioned; D006801:Humans; D015603:Keratinocytes / Q000378:metabolism / Q000821:virology*; D008297:Male; D027383:Papillomaviridae / Q000145:classification / Q000235:genetics*; D017354:Point Mutation; D019098:Telomerase / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
D017077:Culture Media, Conditioned; D016159:Tumor Suppressor Protein p53; D000362:Agar; D019098:Telomerase