[The partial nucleotide sequence of L segment of Hantavirus strain].
OBJECTIVE
To study the nucleotide sequence of L segment of Hantavirus strain A9.


METHODS
The partial L segment cDNA of A9 strain was amplified by PCR, the PCR product was sequenced.


RESULTS
The nucleotide sequence of partial L segment of A9 strain was provided, the sequence was A, T rich, similar to other Hantaviruses, and similar to both M, S segments. Compared with nucleotide sequences from other Hantaviruses, it revealed a higher homology to HTN virus strain 76-118,83.5% at nucleotide level and 96.7% at deduced amino level. SNV, PUU and Tula viruses shared the lower homology. A9 and 76-118 were at same cluster in phylogenetic analysis, close to SEO virus but not to PUU, SNV, Tula viruses. Comparing of partial nucleotide sequence of strain A9 with 76-118, some conserved regions were found, but also some regions of A9 were more conserved to other viruses not 76-118. It suggested that when design the primers for L segments amplification, more than one type of Hantavirus sequence should he concerned. Like the homology from M and S segments of Hantaviruses, the viruses isolated from same host animal showed high homology.


CONCLUSIONS
Nucleotide sequence of L segment of Hantavirus strain A9 revealed homology between 85.2% -70.8% to different types of Hantavirus and shared a higher homology of 85.2% to strain 76-118.
Li
D
D
Institute of Virology, Chinese Academy of Preventive Medicine, Bejing 100052, China.
Liang
M
M
Wang
X
X
D000818:Animals; D001483:Base Sequence; D004270:DNA, Circular / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics*; D006476:Orthohantavirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
D004270:DNA, Circular; D004279:DNA, Viral