title: In vitro bacterial penetration of endodontically treated teeth coronally sealed with a dentin bonding agent.
abstract: This study evaluated the effectiveness of a dentin bonding agent as a barrier to prevent coronal microleakage and examined the effect of a eugenol-based sealer on the sealing ability of this resin adhesive. Fifty-one extracted human mandibular molars were incorporated in a model system using an oral streptococci as a microbial marker. Group 1 consisted of 15 teeth that were obturated with only gutta-percha and received a coronal barrier of Clearfil Liner Bond 2V. Group 2 was identical to group 1, but included the use of a eugenol-based sealer in the obturation. Group 3 consisted of 15 teeth that were obturated with gutta-percha and sealer, but did not receive a coronal barrier. Six teeth served as controls. Bacterial penetration was monitored for 90 days. Results were analyzed after 30, 60, and 90 days with Fisher's exact test (p < 0.05). All controls behaved as expected. Neither group 1 nor group 2 exhibited any bacterial leakage. Eleven of the 15 specimens in group 3 leaked between 15 and 76 days. The coronal barriers in group 1 and group 2 were significantly better in preventing coronal microleakage at 60 days (p = 0.002) and 90 days (p = 0.00005). The presence of eugenol in the sealer had no significant effect on the sealing ability of Clearfil Liner Bond 2V (p = 1).
authors.affiliation: Department of Endodontics, School of Dentistry, Medical College of Georgia, Augusta 30912-1244, USA.
authors.forename: G A
authors.initials: GA
authors.lastname: Wolanek
authors.forename: R J
authors.initials: RJ
authors.lastname: Loushine
authors.forename: R N
authors.initials: RN
authors.lastname: Weller
authors.forename: W F
authors.initials: WF
authors.lastname: Kimbrough
authors.forename: K R
authors.initials: KR
authors.lastname: Volkmann
mesh_terms: D003763:Dental Leakage / Q000382:microbiology / Q000517:prevention & control*; D017220:Dentin-Bonding Agents; D005054:Eugenol; D006801:Humans; D008689:Methacrylates; D008963:Molar; D019279:Resin Cements; D012387:Root Canal Filling Materials / Q000737:chemistry; D013295:Streptococcus mutans
chemical_list: C084544:Clearfil Linerbond System; D017220:Dentin-Bonding Agents; D008689:Methacrylates; D019279:Resin Cements; D012387:Root Canal Filling Materials; D005054:Eugenol