Development and mapping of microsatellites from a microdissected BTA 11-specific DNA library.
A chromosome-specific library was developed for Bos taurus autosome 11 by chromosome microdissection and microcloning using a bovine primary fibroblast culture, obtained from a t(X;23) heifer, that spontaneously developed a translocation chromosome involving bovine chromosome 11. The library was screened using (AC)12 oligos, positive clones selected, sequenced and primers developed to generate bovine chromosome 11-specific microsatellite markers. This study suggests that chromosome-specific libraries have great potential for development of microsatellite markers for the construction of marker-saturated linkage maps for each chromosome.
Ambady
S
S
Department of Veterinary & Animal Science, University of Massachusetts, Amherst, MA 01003, USA.
Kappes
S M
SM
Park
C
C
Ma
R Z
RZ
Beever
J E
JE
Lewin
H A
HA
Smith
T P
TP
Beattie
C W
CW
Basrur
P K
PK
Ponce de León
F A
FA
D000818:Animals; D002417:Cattle / Q000235:genetics*; D002874:Chromosome Mapping; D005260:Female; D015723:Gene Library; D005819:Genetic Markers; D018895:Microsatellite Repeats; D014178:Translocation, Genetic; D014960:X Chromosome
D005819:Genetic Markers