The cAMP response element binding protein-2 (CREB-2) can interact with the C/EBP-homologous protein (CHOP).
cAMP response element binding protein-2 (CREB-2) is a basic leucine zipper (bZIP) factor that was originally described as a repressor of CRE-dependent transcription but that can also act as a transcriptional activator. Moreover, CREB-2 is able to function in association with the viral Tax protein as an activator of the human T-cell leukemia virus type I (HTLV-I) promoter. Here we show that CREB-2 is able to interact with C/EBP-homologous protein (CHOP), a bZIP transcription factor known to inhibit CAAT/enhancer-dependent transcription. Cotransfection of CHOP with CREB-2 results in decreased activation driven by the cellular CRE motif or the HTLV-I proximal Tax-responsive element, confirming that CREB-2 and CHOP can interact with each other in vivo.
Gachon
F
F
Laboratoire Infections Rétrovirales et Signalisation Cellulaire, CNRS EP 2104/Université Montpellier I, Institut de Biologie, 4 Bd Henri IV, 34060 Montpellier, France.
Gaudray
G
G
Thébault
S
S
Basbous
J
J
Koffi
J A
JA
Devaux
C
C
Mesnard
J
J
D051701:Activating Transcription Factor 4; D000818:Animals; D022762:CCAAT-Enhancer-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D019556:COS Cells; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D002522:Chlorocebus aethiops; D015967:Gene Expression Regulation, Viral; D015696:Gene Products, tat / Q000378:metabolism; D015497:HIV-1 / Q000235:genetics; D015368:Human T-lymphotropic virus 1 / Q000235:genetics; D006801:Humans; D016350:Leucine Zippers; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae; D051742:Transcription Factor CHOP; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic; D020798:Two-Hybrid System Techniques; D054322:tat Gene Products, Human Immunodeficiency Virus
C497109:ATF4 protein, human; D022762:CCAAT-Enhancer-Binding Proteins; C497249:DDIT3 protein, human; D015696:Gene Products, tat; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; D054322:tat Gene Products, Human Immunodeficiency Virus; D051701:Activating Transcription Factor 4; D051742:Transcription Factor CHOP