title: Proposed phytic acid standard including a method for its analysis.
abstract: A method is described that accurately and rapidly quantifies the free and total phosphorous content of a commercially available, purified, phytic acid preparation. This allows its use as a standard for phytic acid determinations in foods. The method involves a wet ashing step followed by phosphorous measurement with a 1-amino-2-naphthol-4-sulfonic acid-molybdate reagent in a microplate reader at 660 nm. The procedure can be performed in 3 h with as little as 50 mg sample.
authors.affiliation: Health Canada, Nutrition Research Division, Banting Research Centre, Ottawa, ON.
authors.forename: S P
authors.initials: SP
authors.lastname: Brooks
authors.forename: D
authors.initials: D
authors.lastname: Oberleas
authors.forename: B A
authors.initials: BA
authors.lastname: Dawson
authors.forename: B
authors.initials: B
authors.lastname: Belonje
authors.forename: B J
authors.initials: BJ
authors.lastname: Lampi
mesh_terms: D009682:Magnetic Resonance Spectroscopy; D010758:Phosphorus / Q000032:analysis; D010833:Phytic Acid / Q000032:analysis* / Q000592:standards; D012015:Reference Standards
chemical_list: D010758:Phosphorus; D010833:Phytic Acid