title: Gastric and intestinal phenotypes of gastric carcinoma with reference to expression of brain (fetal)-type glycogen phosphorylase.
abstract: OBJECTIVE
  Although reports have suggested that differentiated gastric carcinomas have different phenotypes, i.e., gastric and intestinal type, this classification is complicated and can be confusing. Our previous studies have demonstrated a close relationship between carcinogenesis in differentiated-type gastric cancer and the expression of brain (fetal)-type glycogen phosphorylase (BGP). The purpose of this study was to investigate the relationship between the mucin phenotype of gastric carcinoma and BGP expression.
  
  
  METHODS
  Ninety-six specimens of gastric carcinoma were studied using specific anti-BGP antibody. Correlation of BGP expression with intestinal and gastric phenotypes was determined with the anti-mucin antibodies, HGM, CD10, and MUC2.
  
  
  RESULTS
  BGP was expressed in 82.6% (38/46) of differentiated type and in 24.0% (12/50) of undifferentiated type carcinomas. The incidence of BGP positivity was significantly greater in the differentiated-type carcinoma than in the undifferentiated type (P < 0.001). The proportions of gastric, mixed and intestinal types in differentiated and undifferentiated gastric carcinomas were 13.0%, 47.8%, and 39.2%, and 56.0%, 32.0%, and 12.0%, respectively. In both differentiated and undifferentiated types, the phenotype of gastric and intestinal mucin expression corresponded very well with BGP expression, that is, more than 90% of carcinomas with gastric type did not express BGP, whereas approximately 90% of carcinomas with intestinal type did express BGP.
  
  
  CONCLUSIONS
  The classification of gastric and intestinal phenotypes of gastric carcinoma in terms of BGP expression was simpler and clearer than such classification in terms of mucin immunohistochemistry. It is suggested that BGP is a useful biomarker for the classification of intestinal and gastric type carcinoma of the human stomach, including classification from the carcinogenetic point of view.
authors.affiliation: Second Department of Surgery, Kumamoto University School of Medicine, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Shimada
authors.forename: H
authors.initials: H
authors.lastname: Matsuzaki
authors.forename: T
authors.initials: T
authors.lastname: Marutsuka
authors.forename: K
authors.initials: K
authors.lastname: Shiomori
authors.forename: M
authors.initials: M
authors.lastname: Ogawa
mesh_terms: D002277:Carcinoma / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D005752:Gastric Mucins / Q000235:genetics*; D005753:Gastric Mucosa / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D025002:Glycogen Phosphorylase, Brain Form / Q000235:genetics*; D006801:Humans; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000378:metabolism; D010641:Phenotype; D013274:Stomach Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism
chemical_list: D005752:Gastric Mucins; D025002:Glycogen Phosphorylase, Brain Form