title: Virulence for BALB/c mice and antigenic diversity of eight Toxoplasma gondii strains isolated from animals and humans in Brazil.
abstract: With the purpose of establishing alternative parameters to determine the virulence of Toxoplasma gondii strains, the antigenic diversity of eight strains of the parasite isolated in Brazil was evaluated. BALB/c mice were inoculated i.p. with 10(0), 10(1), 10(2) and 10(3) tachyzoites from each strain. The mortality and time to death of the animals showed that T. gondii strains may be divided in three groups: three strains resulted in 100% of mortality, 5-10 days post inoculation (DPI); three strains resulted in 100% of mortality, 7-19 DPI and brain cysts were observed in the mice which were inoculated; two strains resulted in 0% of mortality, 30 DPI. The analysis of the antigenic profile of different T. gondii strains through Western blotting, using rabbit antiserum to T. gondii, revealed that most antigens are similar to all strains. The mAb 4C3H4 recognized antigens only in the RH, N, AS28 and ME49 strains.
authors.affiliation: Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: A M
authors.initials: AM
authors.lastname: Ferreira
authors.forename: M S
authors.initials: MS
authors.lastname: Martins
authors.forename: R W
authors.initials: RW
authors.lastname: Vitor
mesh_terms: D000818:Animals; D000916:Antibody Diversity; D015153:Blotting, Western; D001921:Brain / Q000469:parasitology; D001927:Brain Diseases / Q000469:parasitology; D001938:Brazil; D003560:Cysts / Q000469:parasitology; D004285:Dogs; D004591:Electrophoresis, Polyacrylamide Gel; D006041:Goats; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D011817:Rabbits; D012756:Sheep; D014122:Toxoplasma / Q000276:immunology / Q000472:pathogenicity*; D014123:Toxoplasmosis / Q000276:immunology / Q000469:parasitology*