[Determination of sucralose in foods by HPLC].
A method for the determination of sucralose in various foods by RI-HPLC and ion chromatography with a pulsed amperometric detector (PAD-IC) was developed. Chopped or homogenized samples were packed into cellulose tubing with 0.01 mol/L hydrochloric acid containing 10% sodium chloride and were dialyzed against 0.01 mol/L hydrochloric acid for 24 hours. The dialyzate was passed through a Bond Elut ENV cartridge, and the cartridge was washed with 0.2 mol/L NaOH and water. Sucralose was eluted from the cartridge with methanol. The extract was taken to dryness in an evaporator and the residue was re-dissolved in water. Sucralose was separated on an Inertsil ODS-3V column with a mobile phase of acetonitrile-water (15:85) and an RI detector. It was also determined on a CarboPak PA1 column with a mobile phase of 100 mmol/L NaOH-75 mmol/L CH3COONa, using a PAD detector. The recoveries of sucralose from various kinds of foods spiked at 50 micrograms/g and 200 micrograms/g ranged from 88-105%. The detection limit in samples was 10 micrograms/g for RI-HPLC and 1 microgram/g for PAD-IC.
Kobayashi
C
C
Tokyo Metropolitan Research Laboratory of Public Health: 3-24-1, Hyakunin-cho, Shinjuku-ku, Tokyo 169-0073, Japan.
Nakazato
M
M
Yamajima
Y
Y
Ohno
I
I
Kawano
M
M
Yasuda
K
K
D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D005504:Food Analysis / Q000379:methods*; D013395:Sucrose / Q000031:analogs & derivatives / Q000032:analysis*
D013395:Sucrose; C026285:trichlorosucrose