Effects of forced diving on the spleen and hepatic sinus in northern elephant seal pups.
In phocid seals, an increase in hematocrit (Hct) accompanies diving and periods of apnea. The variability of phocid Hct suggests that the total red cell mass is not always in circulation, leading researchers to speculate on the means of blood volume partitioning. The histology and disproportionate size of the phocid spleen implicates it as the likely site for RBC storage. We used magnetic resonance imaging on Northern elephant seals to demonstrate a rapid contraction of the spleen and a simultaneous filling of the hepatic sinus during forced dives (P < 0.0001, R(2) = 0.97). The resulting images are clear evidence demonstrating a functional relationship between the spleen and hepatic sinus. The transfer of blood from the spleen to the sinus provides an explanation for the disparity between the timing of diving-induced splenic contraction ( approximately 1-3 min) and the occurrence of peak Hct (15-25 min). Facial immersion was accompanied by an immediate and profound splenic contraction, with no further significant decrease in splenic volume after min 2 (Tukey-Kramer HSD, P = 0.05). At the conclusion of the dive, the spleen had contracted to 16% of its predive volume (mean resting splenic volume = 3,141 ml +/- 68.01 ml; 3.54% of body mass). In the postdive period, the spleen required 18-22 min to achieve resting volume, indicating that this species may not have sufficient time to refill the spleen when routinely diving at sea, which is virtually continuous with interdive surface intervals between 1 and 3 min.
Thornton
S J
SJ
Department of Zoology, University of British Columbia, Vancouver, BC, Canada V6T 1Z4. sheila.thornton@stonebow.otago.ac.nz
Spielman
D M
DM
Pelc
N J
NJ
Block
W F
WF
Crocker
D E
DE
Costa
D P
DP
LeBoeuf
B J
BJ
Hochachka
P W
PW
D000818:Animals; D004242:Diving; D005260:Female; D006400:Hematocrit; D007091:Image Processing, Computer-Assisted; D008099:Liver / Q000033:anatomy & histology / Q000502:physiology*; D008297:Male; D046048:Seals, Earless / Q000033:anatomy & histology / Q000097:blood / Q000502:physiology*; D013154:Spleen / Q000502:physiology*