Analysis of glutathione by capillary electrophoresis based on sample stacking.
Glutathione is a small peptide, which participates in cellular oxidation-reduction and detoxification. It is present in most biological tissues at different concentrations. The oxidized and reduced forms of the peptide were measured in erythrocytes and myocardial tissue by capillary electrophoresis based on stacking. After tissue homogenization or hemolysis of the red blood cells, the samples were deproteinized with acetonitrile and injected filling about 13% of the capillary volume. The electrophoresis was performed at 10 kV using a separation buffer of 250 mM borate, 50 mM Tris, pH 8.0. Sample stacking increased the sensitivity of detection by 10-20-fold.
Shihabi
Z K
ZK
Department of Pathology, Wake Forest University School of Medicine, Winston-Salem, NC 27157, USA. zshihabi@wfubmc.edu
Hinsdale
M E
ME
Cheng
C P
CP
D000818:Animals; D002021:Buffers; D004285:Dogs; D019075:Electrophoresis, Capillary / Q000379:methods* / Q000706:statistics & numerical data; D004912:Erythrocytes / Q000737:chemistry; D005978:Glutathione / Q000032:analysis* / Q000097:blood; D019803:Glutathione Disulfide / Q000032:analysis / Q000097:blood; D006863:Hydrogen-Ion Concentration; D009206:Myocardium / Q000737:chemistry; D012680:Sensitivity and Specificity
D002021:Buffers; D005978:Glutathione; D019803:Glutathione Disulfide