title: Gangliosides in rat femoral injury: early effect on intimal hyperplasia.
abstract: Previous studies demonstrated that some immunosuppressive agents inhibit arterial intimal hyperplasia. Our previous studies demonstrated that gangliosides (Gang) have an immunosuppressive effect on as well as an anti-inflammatory role in the wound-healing process. Therefore, we decided to examine the effect of Gang on intimal hyperplasia. Twenty Wistar isogenic rats received a transverse division of the anterior wall of the femoral artery, followed by suturing using mononylon 10-0 under surgical microscopy and were then divided into two groups: Gang group, 3 mg/kg per day of Gang, and control group, vehicle, intramuscularly from surgery to death (1 and 3 weeks, respectively). Concentric intimal hyperplasia was observed in arteries stained by hematoxylin-eosin in control and Gang groups. However, the media layer did not demonstrate any major alterations. After 3 weeks, the Gang group showed more intimal hyperplasia than the control group. Therefore, because intimal hyperplasia worsened in the presence of Gang after 3 weeks, further studies will be necessary to clarify its role in intimal proliferation.
authors.affiliation: Operative Technique and Experimental Surgery, Universidade Federal de São Paulo, Escola Paulista de Medicina (UNIFESP-EPM), São Paulo, Brazil.
authors.forename: L C
authors.initials: LC
authors.lastname: Castro
authors.forename: E F
authors.initials: EF
authors.lastname: Montero
authors.forename: M E
authors.initials: ME
authors.lastname: Pedrosa
authors.forename: K
authors.initials: K
authors.lastname: Von Kossel
authors.forename: M J
authors.initials: MJ
authors.lastname: Simões
authors.forename: A J
authors.initials: AJ
authors.lastname: Nigro
mesh_terms: D000714:Anastomosis, Surgical; D000818:Animals; D005263:Femoral Artery / Q000473:pathology / Q000601:surgery*; D005352:Fibromuscular Dysplasia / Q000473:pathology*; D005732:Gangliosides / Q000494:pharmacology*; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D007273:Injections, Intramuscular; D008297:Male; D008866:Microsurgery; D051381:Rats; D017208:Rats, Wistar; D017539:Tunica Intima / Q000473:pathology; D017540:Tunica Media / Q000473:pathology
chemical_list: D005732:Gangliosides; D007166:Immunosuppressive Agents