Pretreatment of mice with clindamycin improves survival of endotoxic shock by modulating the release of inflammatory cytokines.
Suppression of endotoxin release and subsequent production of inflammatory cytokines is crucial in the treatment of septic shock. We investigated the effect of clindamycin (CLI) on endotoxic shock induced in mice by Escherichia coli lipopolysaccharide (LPS). Mice were treated with CLI (160 to 600 mg/kg) or saline and then injected with E. coli LPS and D-(+)-galactosamine intraperitoneally 0.5 h after CLI administration. Pretreatment with CLI significantly improved survival in a dose-dependent manner (CLI, at 160, 300, and 440 mg/kg) and significantly lowered the peak concentrations of tumor necrosis factor alpha and interleukin-1beta (IL-1beta) in serum. However, the peak concentrations of IL-6 in the sera of CLI-treated mice were higher than in control mice. Our findings suggest that CLI alters LPS-induced inflammatory cytokine production and suppresses endotoxin-induced mortality in this murine model.
Hirata
N
N
Second Department of Internal Medicine, Oita Medical University, Hasama, Oita 879-5593, Japan.
Hiramatsu
K
K
Kishi
K
K
Yamasaki
T
T
Ichimiya
T
T
Nasu
M
M
D000818:Animals; D000900:Anti-Bacterial Agents / Q000627:therapeutic use*; D002981:Clindamycin / Q000627:therapeutic use*; D016207:Cytokines / Q000097:blood*; D004195:Disease Models, Animal; D004926:Escherichia coli / Q000737:chemistry; D008070:Lipopolysaccharides; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D012772:Shock, Septic / Q000097:blood / Q000139:chemically induced / Q000401:mortality / Q000517:prevention & control*
D000900:Anti-Bacterial Agents; D016207:Cytokines; D008070:Lipopolysaccharides; D002981:Clindamycin