Acid hydrolase activity of cultured bovine oligodendrocytes.
We measured the activity of several acid hydrolases of cultured oligodendrocytes prepared from adult bovine brain white matter to clarify the biochemical basis of bovine oligodendrocytes in vitro. Lysosomal enzyme activities were assayed by using 4-methylumbelliferyl glycosides as substrates. Lysosomal enzyme activities became higher at 8-11 days in vitro (DIV) than 4 DIV. The enrichment in acid hydrolase specific activities in oligodendrocytes may be associated with lysosomal origin of myelin-like membranes.
Hirayama
M
M
The Second Department of Internal Medicine, Fukui Medical University, Yoshida-gun, Fukui Prefecture, Japan. mihrayama@hospital.kasugai.aichi.jp
Mutoh
T
T
Tokuda
A
A
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D006867:Hydrolases / Q000378:metabolism*; D008247:Lysosomes / Q000201:enzymology; D009836:Oligodendroglia / Q000201:enzymology*; D013997:Time Factors; D005644:alpha-L-Fucosidase / Q000378:metabolism; D001617:beta-Glucosidase / Q000378:metabolism
D006867:Hydrolases; D001617:beta-Glucosidase; D005644:alpha-L-Fucosidase