title: Expedient microdetermination of itraconazole and hydroxyitraconazole in plasma by high-performance liquid chromatography with fluorescence detection.
abstract: The authors describe a useful and rapid micromethod for the analysis of itraconazole (ITZ) and its active metabolite hydroxyitraconazole (HIT) in human plasma. After a simple deproteinization of 100 microL plasma with acetonitrile, the drug, its metabolite, and an internal standard (IS, ketoconazole) were separated on an 8 mm x 10 cm NovaPak (Waters Associates; Milford, MA) C(18) 4-microm particle-size radial compression cartridge. The compounds of interest were detected using a fluorescence detector with the excitation wavelength set at 260 nm and the emission at 365 nm. The mobile phase consisted of 420 mL water adjusted to a pH of 2.5 with phosphoric acid, 580 mL acetonitrile, and 100 microL triethylamine, which was delivered at a flow rate of 3.0 mL/min. This expedient and rugged method is being used to monitor therapeutic levels in bone marrow transplant recipients who are taking the drug for prophylaxis.
authors.affiliation: Pharmacokinetics and Therapeutic Drug Monitoring Section, Biological and Medical Research Department, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia.
authors.forename: S
authors.initials: S
authors.lastname: Al-Rawithi
authors.forename: R
authors.initials: R
authors.lastname: Hussein
authors.forename: I
authors.initials: I
authors.lastname: Al-Moshen
authors.forename: D
authors.initials: D
authors.lastname: Raines
mesh_terms: D000935:Antifungal Agents / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005453:Fluorescence; D006801:Humans; D007202:Indicators and Reagents; D017964:Itraconazole / Q000031:analogs & derivatives* / Q000097:blood*; D015203:Reproducibility of Results
chemical_list: D000935:Antifungal Agents; D007202:Indicators and Reagents; C084794:hydroxyitraconazole; D017964:Itraconazole