title: Comparative effects of nacre water-soluble matrix and dexamethasone on the alkaline phosphatase activity of MRC-5 fibroblasts.
abstract: In this study we demonstrate, for the first time, that dexamethasone and BMP-2 stimulated alkaline phosphatase (ALP) activity in MRC-5 fibroblasts, a cell line derived from human fetal lung. Previously we reported that the water-soluble matrix (WSM) of nacre obtained from the inner shell layer of the oyster Pinctada maxima, promoted an increase in ALP activity that was dose-dependent. In this work, we show that the effect of WSM is also time-dependent. As a comparison, the effect of WSM was also tested in bone marrow stromal cells because marrow and other bone surface-derived osteoblast stem cells have the inherent direct potential for osteogenesis. WSM promotes cell proliferation and ALP activity when tested with bone marrow cells in concentrations between 135 and 540 microg protein/mL. The effect of WSM on ALP activity of bone marrow stromal cells is similar to that obtained by dexamethasone. These results imply that MRC-5 fibroblasts respond to differentiating factors that promote osteoblastic phenotype in bone-derived cell cultures.
authors.affiliation: Laboratoire de Physiologie Générale et Comparée, UMR CNRS 8572, Muséum National d'Histoire Naturelle, 7 rue Cuvier, 75231, Paris Cedex 05, France.
authors.forename: M J
authors.initials: MJ
authors.lastname: Almeida
authors.forename: L
authors.initials: L
authors.lastname: Pereira
authors.forename: C
authors.initials: C
authors.lastname: Milet
authors.forename: J
authors.initials: J
authors.lastname: Haigle
authors.forename: M
authors.initials: M
authors.lastname: Barbosa
authors.forename: E
authors.initials: E
authors.lastname: Lopez
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects* / Q000378:metabolism; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000494:pharmacology*; D002460:Cell Line; D003907:Dexamethasone / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000187:drug effects* / Q000201:enzymology; D005938:Glucocorticoids / Q000494:pharmacology; D006801:Humans; D008297:Male; D010125:Ostreidae / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D012995:Solubility; D013997:Time Factors; D016212:Transforming Growth Factor beta
chemical_list: C525718:BMP2 protein, human; C525720:Bmp2 protein, rat; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D005938:Glucocorticoids; D016212:Transforming Growth Factor beta; D003907:Dexamethasone; D000469:Alkaline Phosphatase