The optimization of isolation media used in immunomagnetic separation methods for the detection of Escherichia coli O157 in foods.
OBJECTIVE
To compare media used in immunomagnetic separation (IMS) techniques for the isolation of Escherichia coli O157 from food.


RESULTS
Foods, both naturally contaminated and spiked, with low numbers (< 1 g(-1)) of stressed E. coli O157 were enriched in media based on buffered peptone water (BPW), tryptone soya and EC broths incubated at 30, 37, 40 and 42 degrees C. Following immunomagnetic separation, beads were plated on a range of selective agars.


CONCLUSIONS
BPW supplemented with vancomycin (8 mg l(-1)) incubated at 42 degrees C, followed by IMS and subsequent plating of immunobeads onto cefixime tellurite sorbitol MacConkey agar plus either Rainbow or CHROMagar agars, proved optimum for the recovery of spiked, stressed E. coli O157 in minced beef, cheese, apple juice and pepperoni. The same protocol was optimum for recovery from naturally-contaminated minced beef and cheese.


CONCLUSIONS
The optimum protocol would increase isolation rates of E. coli O157 from foods.
Ogden
I D
ID
Applied Food Microbiology Group, Department of Medical Microbiology, University of Aberdeen, UK. i.ogden@abdn.ac.uk
Hepburn
N F
NF
MacRae
M
M
D000818:Animals; D001431:Bacteriological Techniques; D001628:Beverages / Q000382:microbiology; D002417:Cattle; D002611:Cheese / Q000382:microbiology; D015169:Colony Count, Microbial; D003470:Culture Media / Q000737:chemistry; D019453:Escherichia coli O157 / Q000254:growth & development* / Q000302:isolation & purification*; D005516:Food Microbiology; D018189:Immunomagnetic Separation / Q000379:methods*; D008460:Meat / Q000382:microbiology; D012680:Sensitivity and Specificity
D003470:Culture Media