[The arboviruses of genus alphavirus family togaviridae were isolated from mosquitoes captured from Yantai].
OBJECTIVE
To isolate arbovirus from mosquitoes caught from Yantai.


METHODS
The isolated viruses were tested for their physico- chemical properties, examined by electron microscopy and specific immuno-reactivity.


RESULTS
Fifteen strains of virus were isolated from mosquitoes in 1994 from Yantai. Three of them were further assayed. The results showed that the viruses could multiply on C6/36 cell and produce typical cytopathogenic effect. The viruses couldn't cause regular sickness and death of suckling mice by intracerebral inoculation. The viruses were sensitive to ether, but resistant to 5, -Idu. One sample was examined with electron microscope, and spherical virus particles were observed. The diameter of the virus particles is about 55 +/- 2. 3nm. It did not react with the JBE virus and Bunyaviridae group specific immuno-ascitic fluids but cross-reacted with the group A Togaviridae specific immuno-ascitic fluid.


CONCLUSIONS
The results showed that the viruses isolated from Yantai belong to alphavirus of togaviridae.
Wang
Y
Y
Yantai Health and Anti-epidemic Station, Yantai 264001, China.
Yang
S
S
Sun
Y
Y
D000818:Animals; D001103:Arboviruses / Q000276:immunology / Q000302:isolation & purification* / Q000648:ultrastructure; D002478:Cells, Cultured; D009033:Culicidae / Q000821:virology*; D051379:Mice; D014035:Togaviridae / Q000276:immunology / Q000302:isolation & purification* / Q000648:ultrastructure