Rapid assay of A1c-type glycosylated hemoglobin in blood of diabetic rats using fast protein liquid chromatography.
OBJECTIVE
To develop a fast protein liquid chromatography (FPLC) method for the assay of Alc-type glycosylated hemoglobin in diabetic rats.


METHODS
Venous blood was collected from rats. After the erythrocytes were washed and sedimented, the hemolysate was prepared and diluted with isotonic saline as a sample for the assay. Samples were then separated on Mono_S_HR_5/5 cation exchange column by a lithium chloride gradient elution system. The Alc-type glycosylated hemoglobin was monitored by measuring the absorbance at 415 nm.


RESULTS
The Alc-type glycosylated hemoglobin was well separated from total hemoglobin. The average HbAlc amount in diabetic rats was determined to be (3.6% +/- 0.6%, n = 7), while the corresponding figure in normal rats was (1.4% +/- 0.4%, n = 7, P < 0.01).


CONCLUSIONS
This FPLC method is easy, rapid and reproducible, and can be used for an assay of diabetic rat HbAlc to evaluate and screen new drugs for diabetes mellitus therapy.
Ge
H
H
State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Science, Beijing 100080, China.
Li
X M
XM
Miao
Z C
ZC
Song
W
W
Liu
S S
SS
D000818:Animals; D002853:Chromatography, Liquid; D003921:Diabetes Mellitus, Experimental / Q000097:blood*; D006442:Glycated Hemoglobin / Q000032:analysis*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
D006442:Glycated Hemoglobin