[The biological effects of recombinant human osteogenic protein-1 to cultured pulp cells].
The study was to observe the biological effects of rhOP-1 on the proliferation, ALPase activity and osteocalcin of dental pulp cells by cell culture technique, MTT, enzyme kinetic methods and radioimmunoassay. The results indicated rhOP-1 could obviously promote the proliferation and enhanced ALPase activity and osteocalcin secretion of pulp cells with showing dose-dependent relationship in this experimental dose range. Thus, rhOP-1 can induce dental pulp cells to differentiate the cells expressing osteoblast phenotype.
Su
Q
Q
College of Stomatology, West China University of Medical Sciences, China.
He
G
G
Sun
Y
Y
Lu
H
H
Wang
S
S
D000469:Alkaline Phosphatase / Q000378:metabolism*; D055419:Bone Morphogenetic Protein 7; D019485:Bone Morphogenetic Proteins / Q000494:pharmacology*; D002455:Cell Division; D002478:Cells, Cultured; D003782:Dental Pulp / Q000166:cytology / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D015675:Osteocalcin / Q000378:metabolism*; D016212:Transforming Growth Factor beta / Q000494:pharmacology
C525819:BMP7 protein, human; D055419:Bone Morphogenetic Protein 7; D019485:Bone Morphogenetic Proteins; D016212:Transforming Growth Factor beta; D015675:Osteocalcin; D000469:Alkaline Phosphatase