title: Affinity screening by packed capillary high performance liquid chromatography using molecular imprinted sorbents. II. Covalent imprinted polymers.
abstract: This study concentrates on the production of covalent molecular imprint polymers (MIPs) as highly selective sorbents for nortriptyline (NOR), a representative tricyclic antidepressant (TCA). The functionalized template contains a polymerizable 4-vinylphenyl carbamate moiety used to bind the template molecule to the polymer matrix. Polymerization with a cross-linker followed by hydrolytic cleavage of the labile carbamate functionality leaves an MIP with selective binding sites capable of binding template through hydrogen bonding interactions. Demonstrated chromatographically through a "selection index", these MIPs showed high selectivity for the template molecule (NOR) among a library of structurally similar compounds. The recognition was found to correlate with structural similarity to the template compound. A direct comparison between covalent and non-covalent molecular imprinting strategies reveals a great deal of improvement in the peak shape of the retained compound resulting from covalent imprinting (evidenced by peak asymmetry factors A.).
authors.affiliation: Department of Chemistry, Oregon State University, Corvallis 97331-4003, USA.
authors.forename: M A
authors.initials: MA
authors.lastname: Khasawneh
authors.forename: P T
authors.initials: PT
authors.lastname: Vallano
authors.forename: V T
authors.initials: VT
authors.lastname: Remcho
mesh_terms: D000929:Antidepressive Agents, Tricyclic / Q000302:isolation & purification; D002846:Chromatography, Affinity / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D009661:Nortriptyline / Q000302:isolation & purification; D011108:Polymers / Q000737:chemistry*
chemical_list: D000929:Antidepressive Agents, Tricyclic; D011108:Polymers; D009661:Nortriptyline