BIACORE J: a new platform for routine biomolecular interaction analysis.
SPR biosensor technology continues to evolve. The recently released platform from Biacore AB (Uppsala, Sweden), BIACORE J, is designed for the routine analysis of biomolecular interactions. Using an antibody-protein A and a ligand-receptor system, we demonstrate the utility of BIACORE J in determining active concentration and binding affinities. The results from these studies illustrate the high sensitivity of the instrument and its ability to generate reproducible binding responses. The BIACORE J is easy to operate and useful in diverse applications, making SPR technology widely accessible as a research tool.
Rich
R L
RL
Center for Biomolecular Interaction Analysis, School of Medicine, University of Utah, Salt Lake City, UT 84132, USA.
Myszka
D G
DG
D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D000937:Antigen-Antibody Reactions; D001666:Binding Sites, Antibody; D015374:Biosensing Techniques; D004305:Dose-Response Relationship, Drug; D004306:Dose-Response Relationship, Immunologic; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D008024:Ligands; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism*; D012015:Reference Standards; D015203:Reproducibility of Results; D013205:Staphylococcal Protein A / Q000378:metabolism*; D020349:Surface Plasmon Resonance / Q000295:instrumentation* / Q000379:methods*; D047990:TNF Receptor-Associated Factor 1; D047992:TNF Receptor-Associated Factor 2; D013997:Time Factors
D000911:Antibodies, Monoclonal; D008024:Ligands; D011506:Proteins; D013205:Staphylococcal Protein A; D047990:TNF Receptor-Associated Factor 1; D047992:TNF Receptor-Associated Factor 2