title: The methionine biosynthetic pathway from homoserine in Pseudomonas putida involves the metW, metX, metZ, metH and metE gene products.
abstract: Biosynthesis of methionine from homoserine in Pseudomonas putida takes place in three steps. The first step is the acylation of homoserine to yield an acyl-L-homoserine. This reaction is catalyzed by the products of the metXW genes and is equivalent to the first step in enterobacteria, gram-positive bacteria and fungi, except that in these microorganisms the reaction is catalyzed by a single polypeptide (the product of the metA gene in Escherichia coli and the met5 gene product in Neurospora crassa). In Pseudomonas putida, as in gram-positive bacteria and certain fungi, the second and third steps are a direct sulfhydrylation that converts the O-acyl-L-homoserine into homocysteine and further methylation to yield methionine. The latter reaction can be mediated by either of the two methionine synthetases present in the cells.
authors.affiliation: Hospital Universitario Virgen de las Nieves, Servicio de Cirugía Pediátrica, Granada, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Alaminos
authors.forename: J L
authors.initials: JL
authors.lastname: Ramos
mesh_terms: D013766:5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase / Q000235:genetics / Q000378:metabolism; D000215:Acylation; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D005798:Genes, Bacterial / Q000235:genetics*; D006714:Homoserine / Q000031:analogs & derivatives / Q000378:metabolism*; D008715:Methionine / Q000096:biosynthesis* / Q000378:metabolism; D008745:Methylation; D009154:Mutation; D016958:Pseudomonas putida / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D006714:Homoserine; D008715:Methionine; D013766:5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase