title: Detection of putative oral pathogens in acute periradicular abscesses by 16S rDNA-directed polymerase chain reaction.
abstract: A 16S rDNA-directed polymerase chain reaction method was used to assess the occurrence of four black-pigmented anaerobic rods, Treponema denticola, and Actinobacillus actinomycetemcomitans in acute periradicular abscesses. Pus was collected by aspiration from 10 cases diagnosed as acute abscesses of endodontic origin. DNA was extracted from the samples and analyzed using a polymerase chain reaction-based identification assay. The method allowed detecting black-pigmented anaerobes in 80% of the examined abscesses. Porphyromonas endodontalis was found in 70%, T. denticola in 50%, Porphyromonas gingivalis in 40%, and Prevotella intermedia in 10% of the cases. P. gingivalis was always found associated with P. endodontalis. Prevotella nigrescens and A. actinomycetemcomitans were not found in any pus sample. The high prevalence of P. endodontalis, T. denticola, and P. gingivalis suggests that they can play an important role in the etiology of acute periradicular abscesses.
authors.affiliation: Department of Dentistry, Estácio de Sá University, Rio de Janeiro, Brazil.
authors.forename: J F
authors.initials: JF
authors.lastname: Siqueira
authors.forename: I N
authors.initials: IN
authors.lastname: Rôças
authors.forename: J C
authors.initials: JC
authors.lastname: Oliveira
authors.forename: K R
authors.initials: KR
authors.lastname: Santos
mesh_terms: D000328:Adult; D016976:Aggregatibacter actinomycetemcomitans / Q000145:classification* / Q000235:genetics; D016301:Alveolar Bone Loss / Q000382:microbiology; D015169:Colony Count, Microbial; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D003731:Dental Caries / Q000382:microbiology; D003790:Dental Pulp Necrosis / Q000382:microbiology; D006801:Humans; D010482:Periapical Abscess / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D017861:Porphyromonas / Q000145:classification* / Q000235:genetics; D016966:Porphyromonas gingivalis / Q000145:classification / Q000235:genetics; D018720:Prevotella / Q000145:classification* / Q000235:genetics; D018713:Prevotella intermedia / Q000145:classification / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000032:analysis / Q000235:genetics; D013492:Suppuration; D014208:Treponema / Q000145:classification* / Q000235:genetics
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S