title: Determination of organic acids of low molecular weight and phosphate in soil by capillary electrophoresis.
abstract: Capillary zone electrophoresis with indirect UV detection at 254 nm was found to be suitable for the determination of organic acids and phosphate in aqueous extracts of soil. The best support electrolyte solution was found to be 10 mM p-hydroxybenzoic acid with 0.5 mM tetradecyltrimethylammonium bromide to reverse electroosmotic flow. This methodology was tested with 9 analytes found in soils: acetate, citrate, formate, phosphate, lactate, oxalate, pyruvate, succinate, and tartrate. The results obtained show that the methodology is adequate for most of the analytes. The sensitivity to oxalate and citrate was low, and the high concentrations of major inorganic anions interfered with the detection of the former. The methodology was applied to the analysis of aqueous extracts of soil samples. Formate, phosphate, lactate, and acetate anions were detected in most of the samples.
authors.affiliation: Universidad de Chile, Facultad de Ciencias Químicas y Farmacéuticas, Santiago.
authors.forename: I
authors.initials: I
authors.lastname: Ahumada
authors.forename: J
authors.initials: J
authors.lastname: Mendoza
authors.forename: P
authors.initials: P
authors.lastname: Escudero
authors.forename: K
authors.initials: K
authors.lastname: Mossert
authors.forename: L
authors.initials: L
authors.lastname: Ascar
mesh_terms: D019342:Acetic Acid / Q000032:analysis; D019343:Citric Acid / Q000032:analysis; D019075:Electrophoresis, Capillary; D005561:Formates / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D019815:Oxalic Acid / Q000032:analysis; D010710:Phosphates / Q000032:analysis*; D012987:Soil / Q000032:analysis*
chemical_list: D005561:Formates; D010710:Phosphates; D012987:Soil; C030544:formic acid; D019343:Citric Acid; D019815:Oxalic Acid; D019342:Acetic Acid