Histochemical identification of carbohydrate moieties in the accessory olfactory bulb of the mouse using a panel of lectins.
Lectin binding patterns in the olfactory bulb of the mouse were investigated using 12 biotinylated lectins. Three, with specificities for galactose, N-acetylgalactosamine and L-fucose, stained only the nervous and glomerular layers of the accessory olfactory bulb; four, with specificities for galactose or N-acetylglucosamine, stained these layers in both the accessory and the main olfactory bulbs; three, with specificities for N-acetylgalactosamine or L-fucose, effected general staining with little contrast between the background and the accessory olfactory bulb or other structures; the remaining two, both of them specific for mannose, stained no part of the tissue studied. In the nervous and glomerular layers of the accessory olfactory bulb six lectins stained the anterior and posterior halves with different intensities and two of these six similarly differentiated between rostral and caudal regions of the posterior half. We conclude that: (i) three lectins binding to different monosaccharides are specific stains for the vomeronasal system when used in this area of the mouse brain; (ii) it may be appropriate to distinguish three parts in the mouse accessory olfactory bulb, instead of the hitherto generally accepted two.
Salazar
I
I
Department of Anatomy and Embryology, Faculty of Veterinary Medicine, E-27002 Lugo, Spain. anigsabe@lugo.usc.es
Sánchez Quinteiro
P
P
Lombardero
M
M
Cifuentes
J M
JM
D000116:Acetylgalactosamine / Q000737:chemistry; D000818:Animals; D019921:Biotinylation; D002241:Carbohydrates / Q000737:chemistry*; D005260:Female; D005643:Fucose / Q000737:chemistry; D005690:Galactose / Q000737:chemistry; D037102:Lectins / Q000737:chemistry*; D051379:Mice; D008807:Mice, Inbred BALB C; D009830:Olfactory Bulb / Q000473:pathology / Q000502:physiology*; D011485:Protein Binding
D002241:Carbohydrates; D037102:Lectins; D005643:Fucose; D000116:Acetylgalactosamine; D005690:Galactose