Use of degenerate oligonucleotide primed PCR (DOP-PCR) for the genotyping of low-concentration DNA samples.
Degenerate oligonucleotide primed amplification (DOP-PCR) is an efficient method for performing whole genome amplification. We analysed the yield of DNA using this technique starting with various quantities of material. We used DOP-PCR products to genotype single nucleotide polymorphisms and insertion/deletion polymorphisms. DOP-PCR also proved usable for SSCP analysis.
Barbaux
S
S
INSERM U 525 et SC7, Paris, France. barbaux@idf.inserm.fr
Poirier
O
O
Cambien
F
F
D001345:Autoradiography; D004247:DNA / Q000032:analysis* / Q000235:genetics*; D005838:Genotype; D009841:Oligonucleotides / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D018807:Polymorphism, Single-Stranded Conformational; D018401:Sample Size; D012680:Sensitivity and Specificity; D017384:Sequence Deletion / Q000235:genetics
D009841:Oligonucleotides; D004247:DNA