Evaluation of the use of Sr2+ in alginate immobilization of cells.
In recent years the method of immobilization of living cells in Ca-alginate beads has gained a wide range of applications. In all cases high chemical stability of the immobilization material and mild conditions for the cells are prerequisites. However, in long-term experiments that may last for several days Ca-alginate may dissolve due to an exchange of Ca2+ with Na+, forming fluid Na-alginate. As well as Ca-alginate, the more chemically stable Sr-alginate and Ba-alginate are materials that have been used for the immobilization of living cells. In this study, the effects of Ca2+, Sr2+ and Ba2+ on growth, viability and intracellular free calcium concentration in a human leukemic T cell line (Jurkat) were investigated. The findings in this study, and the fact that Sr-alginate has a considerably higher chemical stability than Ca-alginate, led to the conclusion that Sr-alginate is a more suitable material for use in the entrapment of living cells in long-term studies.
Widerøe
H
H
Department of Physics, Norwegian University of Science and Technology, Trondheim. Hege.Wideroe@phys.ntnu.no
Danielsen
S
S
D000464:Alginates; D017609:Barium Compounds / Q000494:pharmacology*; D002122:Calcium Chloride / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D018914:Cells, Immobilized / Q000166:cytology* / Q000187:drug effects; D002712:Chlorides / Q000494:pharmacology*; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D006801:Humans; D019169:Jurkat Cells; D013324:Strontium / Q000494:pharmacology*; D013601:T-Lymphocytes
D000464:Alginates; D017609:Barium Compounds; D002712:Chlorides; D006603:Hexuronic Acids; C024986:barium chloride; D020723:Glucuronic Acid; C025700:strontium chloride; D002122:Calcium Chloride; D013324:Strontium