title: Centrosomal association of histone macroH2A1.2 in embryonic stem cells and somatic cells.
abstract: The histone 2A variant macroH2A1.2 is expressed in female and male mammals and is implicated in X-chromosome inactivation and autosomal gene silencing. In undifferentiated and early differentiating murine embryonic stem (ES) cells a cytosolic pool of macroH2A1.2 has recently been reported and found to be associated with the centrosome. Here, we show that the centrosomal association of macroH2A1.2 is a widespread phenomenon and is not restricted to undifferentiated and early differentiating ES cells. By indirect immunofluorescence we detect macroH2A1.2 protein in a juxtanuclear structure that duplicates once per cell cycle and colocalizes with centrosomal gamma-tubulin in both XX and XY ES cells prior to and throughout their differentiation. MacroH2A1.2 localization to the centrosome is also observed in female and male somatic cells, both in interphase and in mitosis. Biochemical analysis demonstrates that the association between macroH2A1.2 and the centrosome in somatic cells is stable, as macroH2A1.2 copurifies with centrosomes isolated from human lymphoblasts. Therefore, in addition to a nuclear pool of macroH2A1.2 a fraction of the histone is associated with the centrosome in various cell types and throughout ES cell differentiation.
authors.affiliation: X-Inactivation Group, MRC Clinical Sciences Centre, Imperial College School of Medicine, Hammersmith Hospital, London W12 ONN, United Kingdom. jmermoud@hgmp.mrc.ac.uk
authors.forename: J E
authors.initials: JE
authors.lastname: Mermoud
authors.forename: A M
authors.initials: AM
authors.lastname: Tassin
authors.forename: J R
authors.initials: JR
authors.lastname: Pehrson
authors.forename: N
authors.initials: N
authors.lastname: Brockdorff
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D018385:Centrosome / Q000737:chemistry*; D004303:Dosage Compensation, Genetic; D004622:Embryo, Mammalian / Q000166:cytology; D005260:Female; D006657:Histones / Q000302:isolation & purification*; D008297:Male; D051379:Mice; D013234:Stem Cells / Q000166:cytology; D014960:X Chromosome / Q000737:chemistry; D014998:Y Chromosome / Q000737:chemistry
chemical_list: D006657:Histones; C120756:macroH2A histone