Fast analysis of antibacterial isothiazolones by capillary electrophoresis.
Some technical aspects influencing the total time of CE analysis are discussed. A high throughput electrophoretic system based on micellar electrokinetic chromatography (MEKC) is demonstrated as an example. A short capillary, strong electric field, alkaline buffer (pH 9.5) generating strong electroosmotic flow, and parallel hydrodynamic pressure allow the separation of two uncharged isothiazolone derivatives within 45 s.
Barták
P
P
Centre of Bioanalytical Research, Palacký University, Olomouc, Czech Republic. bartak@risc.upol.cz
Bednár
P
P
Friedecký
D
D
Haviger
A
A
Sevcík
J
J
D000890:Anti-Infective Agents / Q000032:analysis*; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D013844:Thiazoles / Q000032:analysis*
D000890:Anti-Infective Agents; D013844:Thiazoles