title: Design of RNA-binding proteins and ligands.
abstract: The rapidly expanding database of RNA structures and protein complexes is beginning to lead to the successful design of specific RNA-binding molecules. Recent combinatorial and structure-based approaches have utilized known nucleic-acid-binding scaffolds from both proteins and small molecules to display a relatively small set of functional groups often used in protein--RNA recognition. Several studies have shown that the tethering of multiple binding modules can enhance RNA-binding affinity and specificity, a strategy also commonly used in DNA recognition.
authors.affiliation: Department of Biochemistry and Biophysics, University of California San Francisco, 94143-0448, USA. frankel@cgl.ucsf.edu
authors.forename: A C
authors.initials: AC
authors.lastname: Cheng
authors.forename: V
authors.initials: V
authors.lastname: Calabro
authors.forename: A D
authors.initials: AD
authors.lastname: Frankel
mesh_terms: D000818:Animals; D020650:Combinatorial Chemistry Techniques; D008024:Ligands; D008958:Models, Molecular; D009414:Nerve Growth Factors; D019151:Peptide Library; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry*; D016601:RNA-Binding Proteins / Q000737:chemistry* / Q000378:metabolism*; D013329:Structure-Activity Relationship; D015534:Trans-Activators / Q000737:chemistry*; D016335:Zinc Fingers
chemical_list: D008024:Ligands; C099695:MANF protein, human; D009414:Nerve Growth Factors; D019151:Peptide Library; D011506:Proteins; D016601:RNA-Binding Proteins; D015534:Trans-Activators