The lateral flow card test: an alternative method for the detection of Trichinella infection in swine.
A novel lateral flow card (TS-Card pork) test was developed for the serological detection of Trichinella infected pigs. Based on extensive studies performed in Romania during 1999-2000 this test proved to be highly specific sensitive, rapid (3-12 minutes) and easy to use (no need for laboratory facilities). It can be used both for the detection of Trichinella infection in carcasses and for epizooliological studies using a variety of samples including whole or dried blood, serum, or tissue fluids. The TS-Card pork test, used as a screening test, can be the foundation of an on-farm or field based inspection system to significantly improve food safety in countries with a high prevalence of Trichinella in pigs or other food animal species. The results presented are also promising for application of the test in an on-line laboratory based inspection system since the speed of the test allows sufficient time to rail out suspected hog carcasses during the slaughter process.
Patrascu
I
I
A.R.T.E. International SRL, Pascal Cristian 33, 77713 Bucharest, Romania. arte-med@cabletv.ro
Gamble
H R
HR
Sofronic-Milosavljevic
L
L
Radulescu
R
R
Andrei
A
A
Ionescu
V
V
Timoceanu
V
V
Boireau
P
P
Cuperlovic
K
K
Djordjevic
M
M
Murrell
K D
KD
Noeckler
K
K
Pozio
E
E
D000818:Animals; D000909:Antibodies, Helminth / Q000032:analysis / Q000097:blood; D004311:Double-Blind Method; D004797:Enzyme-Linked Immunosorbent Assay; D005838:Genotype; D008460:Meat / Q000469:parasitology*; D011934:Reagent Strips; D015203:Reproducibility of Results; D012383:Romania; D012680:Sensitivity and Specificity; D013552:Swine; D013553:Swine Diseases / Q000097:blood / Q000175:diagnosis*; D014234:Trichinella / Q000235:genetics / Q000302:isolation & purification*; D014235:Trichinellosis / Q000097:blood / Q000175:diagnosis / Q000662:veterinary*
D000909:Antibodies, Helminth; D011934:Reagent Strips