Structural analysis of the exopolysaccharide from Burkholderia caribensis strain MWAP71.
Burkholderia caribensis strain MWAP71 was isolated from rhizosphere soil microaggregates in Martinique. The extracellular polysaccharide produced by this strain was found to be composed of D-glucose (D-Glc), 6-deoxy-L-talose (L-6dTal), 3-deoxy-D-manno-oct-2-ulosonic acid (Kdo), and an O-acetyl group in a molar ratio of 2:1:1:1. The primary structure of the polysaccharide was shown by sugar analysis, electrospray mass spectrometry, partial acid hydrolysis and 1-D and 2-D NMR spectroscopy to consist of a tetrasaccharide repeating unit having the following structure: [structure in text].
Vanhaverbeke
C
C
Centre de Recherches sur les Macromolécules Végétales, CNRS and Université Joseph Fourier, B.P.53, F-38041, Grenoble, France.
Heyraud
A
A
Achouak
W
W
Heulin
T
T
D019117:Burkholderia / Q000737:chemistry*; D003837:Deoxy Sugars / Q000737:chemistry*; D005947:Glucose / Q000737:chemistry*; D006601:Hexoses / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D011135:Polysaccharides, Bacterial / Q000032:analysis / Q000737:chemistry*; D012988:Soil Microbiology; D013045:Species Specificity; D021241:Spectrometry, Mass, Electrospray Ionization; D013400:Sugar Acids / Q000737:chemistry*
D003837:Deoxy Sugars; D006601:Hexoses; D011135:Polysaccharides, Bacterial; D013400:Sugar Acids; C011754:6-deoxytalose; D005947:Glucose; C120356:3-deoxy-manno-oct-2-ulopyranosonic acid