Antimurine immunoglobulin antibody responses after the administration of murine monoclonal antibodies to rats are altered by small bowel allograft rejection.
BACKGROUND
This study monitored the induction of antimurine immunoglobulin antibody responses after the administration of anti-CD4 (OX38) and anti-LFA-1 (WT.1) monoclonal antibodies to DA rats.


METHODS
Monoclonal antibody was administered i.v. on 3 consecutive days to untransplanted DA rats, and DA recipients of PVG small bowel allografts. Control animals received no monoclonal antibody. Antimurine immunoglobulin antibody levels in serum samples were determined by enzyme immunoassay.


RESULTS
No antimurine immunoglobulin antibody was detected in untransplanted animals receiving OX38 alone. Reactivity was apparent in WT.1-treated animals, but this response was totally abrogated by the co-administration of OX38. A combination of OX38 and WT.1 had no effect on allograft recipient survival and antimurine immunoglobulin antibody responses were detected in all allograft recipients, irrespective of the treatment regimen.


CONCLUSIONS
Although OX38 inhibited the antibody response both to itself and to WT.1 in untransplanted animals, the immune reaction induced by small bowel allograft rejection overcame this inhibitory capacity.
Bowles
M J
MJ
Division of Clinical Sciences (NGH), Clinical Sciences Centre (University of Sheffield), Northern General Hospital, Herries Road, Sheffield S5 7AU, UK.
Wood
R M
RM
Pockley
A G
AG
D000818:Animals; D000910:Antibodies, Heterophile / Q000097:blood*; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D000917:Antibody Formation; D015704:CD4 Antigens / Q000276:immunology*; D006084:Graft Rejection / Q000276:immunology*; D006085:Graft Survival / Q000187:drug effects / Q000276:immunology*; D007421:Intestine, Small / Q000276:immunology*; D016169:Lymphocyte Function-Associated Antigen-1 / Q000276:immunology*; D008297:Male; D051379:Mice; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors; D016042:Transplantation, Heterotopic; D014184:Transplantation, Homologous / Q000276:immunology*
D000910:Antibodies, Heterophile; D000911:Antibodies, Monoclonal; D015704:CD4 Antigens; D016169:Lymphocyte Function-Associated Antigen-1; C108170:OX-38 monoclonal antibody