DNA vaccination against v-src oncogene-induced tumours in congenic chickens.
DNA vaccination is particularly efficient for induction of cytotoxic T-lymphocyte (CTL) response. In our experiments, we used MHC(B) congenic chicken lines CB and CC (regressors and progressors of v-src-induced tumours, respectively) and a mutated, non-oncogenic v-src gene construct as the DNA vaccine. A high degree of vaccine protection against oncogenic v-src challenge was achieved in the CB line chickens. CTL response was demonstrated in vitro and by adoptive transfer of immune cells to the syngeneic host and to the CC line chickens rendered tolerant to CB cells. In the CC line chickens we observed tumour growth retardation after a low-dose DNA vaccination administered to immature recipients while higher amounts of DNA vaccine in immunocompetent chickens exerted an enhancing effect.
Plachý
J V
JV
Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Flemingovo nam. 2, 16637 Prague 6, Czech Republic. plachy@img.cas.cz
Hejnar
J V
JV
Trtková
K
K
Trejbalová
K
K
Svoboda
J
J
Hála
K
K
D019264:Adoptive Transfer / Q000379:methods; D000367:Age Factors; D000818:Animals; D020296:Animals, Congenic; D001358:Avian Sarcoma Viruses / Q000235:genetics / Q000276:immunology*; D002472:Cell Transformation, Viral; D002642:Chick Embryo; D002645:Chickens; D004306:Dose-Response Relationship, Immunologic; D016391:Genes, src / Q000235:genetics / Q000276:immunology*; D015688:Oncogene Protein pp60(v-src) / Q000276:immunology*; D001357:Sarcoma, Avian / Q000276:immunology* / Q000517:prevention & control*; D019444:Vaccines, DNA / Q000627:therapeutic use*; D014765:Viral Vaccines / Q000235:genetics / Q000627:therapeutic use*
D019444:Vaccines, DNA; D014765:Viral Vaccines; D015688:Oncogene Protein pp60(v-src)