The use of a synthetic antigen for the serological diagnosis of human trichinellosis.
Hosts infected with Trichinella produce antibodies specific for an epitope common to the TSL-1 family antigens. This epitope contained uncommon terminal 3, 6-dideoxy-D-arabinohexose (so called tyvelose) residues. The disaccharide moiety was synthesized and an immunodiagnostic assay was developed, which was specific and sensitive in swine trichinellosis. We aimed to verify the specificity and sensitivity of this immunodiagnostic test in human trichinellosis. 15 sera from normal subjects, 12 from patients with other parasitic diseases and 50 from trichinellosis patients were tested. Indirect enzyme linked immunosorbent assay (ELISA) for specific IgG and an amplified ELISA for specific IgE were performed using beta-tyvelose-GalNAc-bovine serum albumin (BSA) disaccharide conjugate or T. spiralis muscle larvae excretory/secretory (E/S) products, as antigens. Neither control sera nor other parasitic infection sera resulted positive both for IgG and IgE when synthetic or E/S antigens were used. In trichinellosis patient sera, specific IgG were present in 100% of cases, irrespective of the antigen used, but whereas specific IgE were detected in 78% using E/S antigens, a 100% positivity rate was obtained, using the beta-tyvelose-BSA conjugate.
Bruschi
F
F
Dipartimento di Patologia Sperimentale, B.M.I.E., Università di Pisa, Via Roma 55, 56126 Pisa, Italy. fbruschi@med.unipi.it
Moretti
A
A
Wassom
D
D
Piergili Fioretti
D
D
D000909:Antibodies, Helminth / Q000097:blood*; D000917:Antibody Formation; D000947:Antigens, Helminth / Q000276:immunology*; D004187:Disaccharides / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D006601:Hexoses / Q000276:immunology; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D012016:Reference Values; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods; D014235:Trichinellosis / Q000097:blood / Q000175:diagnosis* / Q000276:immunology
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D004187:Disaccharides; D000939:Epitopes; D006601:Hexoses; D007074:Immunoglobulin G; D007073:Immunoglobulin E; C009616:tyvelose