Real-time confocal imaging, during active air abrasion -- substrate cutting.
Air abrasion cutting, using particulates accelerated in a controlled compressed gas stream, is currently being re-evaluated as a precision tissue removal technique for dental cavity preparation. The minimal vibrations and heat generated during cutting commend the technique for use in the shaping of fragile or brittle materials that are vulnerable to vibrations and thermal stresses. Traditional air abrasion studies have relied solely upon post-procedure imaging, and cutting process details have been inferred from the nature of the residual surface. In this paper, however, a real-time confocal microscopic imaging method is described, which for the first time has allowed prior target structure characterization with subsequent imaging of cutting interactions and substrate failure patterns. Using internally focusing long working distance Hill objective lenses, focusing deep to a protective microscope slide and adhesive interfaces, unhindered remote image sampling within the bulk of specimens such as tooth tissue, acrylic and brittle ceramics was possible. Moreover, areas of active cutting and inactive regions were identified within air abraded cavities during their creation. The characteristics of the finished cut surfaces were demonstrated and confirmed the findings of previous SEM studies. The method allowed direct control over all the known variables influencing cutting with particulate streams.
Cook
R J
RJ
Department of Microscopy & Imaging, GKT Dental Institute, Floor 17 Guy's Tower, Guy's Hospital, London Bridge, London SE1 9RT, UK.richard_james.cook@kcl.ac.uk
Azzopardi
A
A
Thompson
I D
ID
Watson
T F
TF
D020906:Air Abrasion, Dental / Q000295:instrumentation*; D003737:Dental Cavity Preparation / Q000295:instrumentation* / Q000379:methods*; D003743:Dental Enamel / Q000648:ultrastructure; D003762:Dental Instruments; D003804:Dentin / Q000648:ultrastructure; D006801:Humans; D018613:Microscopy, Confocal / Q000379:methods; D014070:Tooth / Q000648:ultrastructure*