title: [Molecular epidemiological study on HIV-1 infection in Shandong Province].
abstract: OBJECTIVE
  The aim of this study was to understand the distribution of HIV-1 strain subtypes and their variation in Shandong Province and to analyze their source and predict their epidemic trend.
  
  
  METHODS
  We collected the whole blood from 25 anti-HIV-1 antibody positive cases and separated the peripheral blood mononuclear cells. The provirus DNA was extracted and amplified by nested-PCR, after which the HIV-1 membrane protein gene (env) C2-V3 region was sequenced and its subtype was analyzed.
  
  
  RESULTS
  Among 25 HIV-1 antibody positive cases, 24 HIV-1 env gene fragments were amplified and analyzed.5 subtypes of Group HIV-1 M were identified among these cases,that are subtypes A, B, B', C and E. 13 occupational blood donors were all classified as subtype B' (Thailand B'). A, B, C or E subtypes were discovered in 10 returned laborers working abroad and 1 female spouse of one laborer.
  
  
  CONCLUSIONS
  The condition of HIV-1 subtype infection was complicated in Shandong Province. So, it is important to strengthen the surveillance and control of HIV-1 subtype strain infection in high risk populations especially the occupational blood donors and returned laborers working abroad to prevent its spread in Shandong Province.
authors.affiliation: Shandong Provincial Health and Anti-epidemic Station, Jinan 250014, China.
authors.forename: C
authors.initials: C
authors.lastname: Liu
authors.forename: H
authors.initials: H
authors.lastname: Xing
authors.forename: S
authors.initials: S
authors.lastname: Su
mesh_terms: D000328:Adult; D001782:Blood Donors; D002681:China / Q000453:epidemiology; D005260:Female; D015752:Genes, env / Q000235:genetics*; D005838:Genotype; D015658:HIV Infections / Q000453:epidemiology* / Q000821:virology; D015497:HIV-1 / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008297:Male; D017720:Molecular Epidemiology; D017422:Sequence Analysis, DNA