title: Use of a hyaluronate membrane for jejunal anastomosis in horses.
abstract: OBJECTIVE
  To compare the outcomes of double-layer inverting anastomosis (DIA), single-layer anastomosis (SLA), and single-layer anastomosis combined with a hyaluronate membrane (SLA+HA-membrane) with respect to stomal diameter, adhesion formation, surgery time, and anastomotic healing in horses.
  
  
  METHODS
  18 adult horses.
  
  
  METHODS
  Midline celiotomy and end-to-end anastomoses were performed. In control horses (n = 6), DIA was performed; in treated horses, SLA was performed (6) or SLA+HA-membrane was performed (6). Horses were euthanatized 21 days after surgery. Abdominal adhesions were evaluated grossly and histologically. Stomal diameters were measured ultrasonographically and compared with adjacent luminal diameters. Anastomotic healing was evaluated histologically for fibrosis and inflammation, tissue alignment, and inversion. Surgery times were recorded for the anastomotic procedure and compared among groups.
  
  
  RESULTS
  There were significantly more adhesions in the SLA group, compared with the DIA and SLA+HA-membrane groups. Reduction in stomal diameters in the DIA group was significantly greater than the SLA and SLA+HA-membrane groups. Surgery times for the DIA group were significantly greater than the SLA and SLA+HA-membrane groups. Histologic findings of fibrosis, inflammation, and mucosal healing were similar among groups. There was significant tissue inversion in the DIA group, compared with the 2 treatment groups. Tissue alignment was not different among groups.
  
  
  CONCLUSIONS
  Use of a SLA+HA-membrane was an effective small intestinal anastomotic technique. This technique was faster to perform and resulted in a larger stomal diameter, compared with the DIA technique and significantly fewer perianastomotic adhesions, compared with the SLA technique.
authors.affiliation: Department of Large Animal Medicine, College of Veterinary Medicine, University of Georgia, Athens 30602, USA.
authors.forename: R B
authors.initials: RB
authors.lastname: Eggleston
authors.forename: E
authors.initials: E
authors.lastname: Mueller
authors.forename: J E
authors.initials: JE
authors.lastname: Quandt
authors.forename: L
authors.initials: L
authors.lastname: Neuwirth
authors.forename: B G
authors.initials: BG
authors.lastname: Harmon
authors.forename: W R
authors.initials: WR
authors.lastname: Waguespack
authors.forename: M E
authors.initials: ME
authors.lastname: Rainbow
mesh_terms: D000714:Anastomosis, Surgical / Q000379:methods / Q000662:veterinary; D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D006651:Histocytochemistry / Q000662:veterinary; D006736:Horses / Q000601:surgery*; D006820:Hyaluronic Acid / Q000494:pharmacology*; D007583:Jejunum / Q000000981:diagnostic imaging / Q000473:pathology / Q000601:surgery*; D011897:Random Allocation; D054047:Surgical Stomas / Q000662:veterinary; D014463:Ultrasonography
chemical_list: D001672:Biocompatible Materials; D006820:Hyaluronic Acid