title: Second-generation Hybrid capture test and Amplicor monitor test generate highly correlated hepatitis B virus DNA levels.
abstract: The performance of the Digene Hybrid Capture II HBV DNA Test HC II and the Roche Cobas Amplicor Monitor Test (Cobas-HBV) was evaluated on 252 serum samples. One hundred and seventy-three samples were HBV DNA positive and 75 HBV DNA negative by both assays. Four samples were HBV DNA positive by Cobas-HBV only. Linear regression analysis showed that the HBV DNA concentrations obtained from both assays were significantly related (n=173, r=0.976, P<0.0001). The results of the study show that Hybrid capture II and Cobas-HBV could be used equally in the management for patients with chronic HBV infection.
authors.affiliation: Medical Faculty of Ljubljana, Institute of Microbiology and Immunology, Zaloska 4, 1105, Ljubljana, Slovenia. mario.poljak@mf.uni-lj.si
authors.forename: M
authors.initials: M
authors.lastname: Poljak
authors.forename: I J
authors.initials: IJ
authors.lastname: Marin
authors.forename: K
authors.initials: K
authors.lastname: Seme
authors.forename: V
authors.initials: V
authors.lastname: Brinovec
authors.forename: M
authors.initials: M
authors.lastname: Maticic
authors.forename: J
authors.initials: J
authors.lastname: Meglic-Volkar
authors.forename: G
authors.initials: G
authors.lastname: Lesnicar
authors.forename: A
authors.initials: A
authors.lastname: Vince
mesh_terms: D001331:Automation; D004279:DNA, Viral / Q000097:blood*; D006515:Hepatitis B virus / Q000235:genetics* / Q000302:isolation & purification*; D019694:Hepatitis B, Chronic / Q000097:blood / Q000175:diagnosis* / Q000821:virology*; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014773:Virology / Q000379:methods
chemical_list: D004279:DNA, Viral