Determination of residues of enrofloxacin and its metabolite ciprofloxacin in biological materials by capillary electrophoresis.
A method for the analysis of enrofloxacin and ciprofloxacin in chicken muscle using marbofloxacin as internal standard is proposed. Clean-up and pre-concentration of the samples are effected by solid-phase extraction and determination is carried out by capillary electrophoresis using a photodiode array detector. The calibration graphs are linear for enrofloxacin and ciprofloxacin from 10 to 300 microg/kg. The method recoveries for enrofloxacin and ciprofloxacin are 74 and 54%, respectively. The limit of detection for the two compounds is lower than 25 microg/kg, which allows the detection of positive muscle samples at the required maximum residue limits.
Barrón
D
D
Department  Analytical Chemistry, University of Barcelona, Spain.
Jiménez-Lozano
E
E
Cano
J
J
Barbosa
J
J
D000818:Animals; D000890:Anti-Infective Agents / Q000032:analysis*; D002138:Calibration; D002645:Chickens; D002939:Ciprofloxacin / Q000032:analysis*; D004350:Drug Residues / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods*; D000077422:Enrofloxacin; D024841:Fluoroquinolones; D006863:Hydrogen-Ion Concentration; D009132:Muscles / Q000737:chemistry*; D015363:Quinolones / Q000032:analysis*; D012015:Reference Standards
D000890:Anti-Infective Agents; D024841:Fluoroquinolones; D015363:Quinolones; D000077422:Enrofloxacin; D002939:Ciprofloxacin