title: Emergence of a pneumococcal clone with cephalosporin resistance and penicillin susceptibility.
abstract: We report two South African serotype 6B pneumococcal isolates with cephalosporin resistance, yet with susceptibility to penicillin. DNA fingerprinting revealed that they were clonal in origin. pbp 2X and 1A genes showed major alterations typical of cephalosporin-resistant pneumococci. The pbp 2B gene was completely unaltered, explaining the penicillin susceptibility of the isolates.
authors.affiliation: MRC/SAIMR/WITS Pneumococcal Diseases Research Unit, Department of Clinical Microbiology and Infectious Diseases, South African Institute for Medical Research, Johannesburg, South Africa. anthonys@mail.saimr.wits.ac.za
authors.forename: A M
authors.initials: AM
authors.lastname: Smith
authors.forename: R F
authors.initials: RF
authors.lastname: Botha
authors.forename: H J
authors.initials: HJ
authors.lastname: Koornhof
authors.forename: K P
authors.initials: KP
authors.lastname: Klugman
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D002352:Carrier Proteins / Q000235:genetics*; D018550:Cephalosporin Resistance / Q000235:genetics*; D006602:Hexosyltransferases / Q000235:genetics*; D006801:Humans; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000235:genetics*; D002267:Muramoylpentapeptide Carboxypeptidase; D046915:Penicillin-Binding Proteins; D010406:Penicillins / Q000494:pharmacology*; D010458:Peptidyl Transferases / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D013019:South Africa; D013296:Streptococcus pneumoniae / Q000187:drug effects / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D009097:Multienzyme Complexes; D046915:Penicillin-Binding Proteins; D010406:Penicillins; C072223:PBP 2x protein, Streptococcus; D010458:Peptidyl Transferases; D006602:Hexosyltransferases; D002267:Muramoylpentapeptide Carboxypeptidase