title: [An AFLP marker related to fibrogenesis in upland cotton (Gossypium hirsuturm L.)].
abstract: By using AFLP technique polymorphism analysis was performed between a fuzzlesslintless mutant line and its isogenic wild-type line, Xuzhou 142. Out of 6,360 bands produced by 64 pairs primers, a fragment, named as CF1, appearing stably in wild-type line, Xuzhou 142. This polymorphism was further verified using several normal fiber varieties and F2, F3 populations from the cross of fuzzless-lintless mutant line with a high-lint-percentage variety Yumian No. 1. The cosegregation of CF1 and fibrogenesis was proved, which suggested that CF1 can be used as a molecular marker for cotton fibrogenesis. The CF1 segment was cloned into PUCm-T Vector and then sequenced. The putative amino acid sequences, is an analogue to phenol hydroxylase alpha subunit, outer surface protein C, NADH dehydrogenase subunit 1, NADH-ubiquinone oxidoreductase, 2-oxoacid ferredoxin oxidoreduct and hypothetical 14.5kD protein.
authors.affiliation: Biotechnology Research Center, Southwest Agricultural University, Chongqing 400716, China. peiyan@swau.edu.cn
authors.forename: M
authors.initials: M
authors.lastname: Luo
authors.forename: M Y
authors.initials: MY
authors.lastname: Li
authors.forename: L
authors.initials: L
authors.lastname: Hou
authors.forename: Y H
authors.initials: YH
authors.lastname: Xiao
authors.forename: Z S
authors.initials: ZS
authors.lastname: Zhang
authors.forename: Y
authors.initials: Y
authors.lastname: Pei
authors.forename: D F
authors.initials: DF
authors.lastname: Ji
mesh_terms: D001483:Base Sequence; D015415:Biomarkers; D005355:Fibrosis; D003368:Gossypium / Q000235:genetics*; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic
chemical_list: D015415:Biomarkers