Frequent demonstration of human herpesvirus 8 (HHV-8) in bone marrow biopsy samples from Turkish patients with multiple myeloma (MM).
In order to investigate the frequency of HHV-8 in MM patients from another geographic location, we obtained fresh bone marrow (BM) biopsies from Turkish patients with MM (n = 21), monoclonal gammopathy of undetermined significance (MGUS) (n = 2), plasmacytoma (n = 1) with BM plasma cell infiltration, various hematological disorders (n = 6), and five healthy Turkish controls. The frequency of HHV-8 was analyzed by polymerase chain reaction (PCR) in two independent laboratories in the USA and in Turkey. Using fresh BM biopsies, 17/21 MM patients were positive for HHV-8 whereas all five healthy controls, and six patients with other hematological disorders were negative. Two patients with MGUS, and one patient with a solitary plasmacytoma were also negative. The data from the two laboratories were completely concordant. Also using primer pairs for v IRF and v IL-8R confirmed the results observed with the KS330233 primers. Furthermore, sequence analysis demonstrated a C3 strain pattern in the ORF26 region which was also found in MM patients from the US. Thus, HHV-8 is present in the majority of Turkish MM patients, and the absence of the virus in healthy controls further supports its role in the pathogenesis of MM.
Beksac
M
M
Ibni Sina Hospital, Department of Hematology, Ankara University School of Medicine, Sihhiye, Turkey.
Ma
M
M
Akyerli
C
C
DerDanielian
M
M
Zhang
L
L
Liu
J
J
Arat
M
M
Konuk
N
N
Koc
H
H
Ozcelik
T
T
Vescio
R
R
Berenson
J R
JR
D001706:Biopsy; D001853:Bone Marrow / Q000473:pathology* / Q000821:virology*; D006566:Herpesviridae Infections / Q000453:epidemiology / Q000821:virology*; D019288:Herpesvirus 8, Human / Q000302:isolation & purification*; D006801:Humans; D009101:Multiple Myeloma / Q000453:epidemiology / Q000209:etiology / Q000473:pathology / Q000821:virology*; D014421:Turkey / Q000453:epidemiology