title: Structure of a legume lectin from the bark of Robinia pseudoacacia and its complex with N-acetylgalactosamine.
abstract: The structure of the bark lectin RPbAI (isoform A4) from Robinia pseudoacacia has been determined by protein crystallography both in the free form and complexed with N-acetylgalactosamine. The free form is refined at 1.80 A resolution to an R-factor of 18.9% whereas the complexed structure has an R-factor of 19.7% at 2.05 A resolution. Both structures are compared to each other and to other available legume lectin structures. The polypeptide chains of the two structures exhibit the characteristic legume lectin tertiary fold. The quaternary structure resembles that of the Phaseolus vulgaris lectin, the soybean agglutinin, and the Dolichos biflorus lectin, but displays some unique features leading to the extreme stability of this lectin.
authors.affiliation: Laboratory of Analytical Chemistry and Medicinal Physicochemistry, Faculty of Pharmaceutical Sciences, Leuven, Belgium.
authors.forename: A
authors.initials: A
authors.lastname: Rabijns
authors.forename: C
authors.initials: C
authors.lastname: Verboven
authors.forename: P
authors.initials: P
authors.lastname: Rougé
authors.forename: A
authors.initials: A
authors.lastname: Barre
authors.forename: E J
authors.initials: EJ
authors.lastname: Van Damme
authors.forename: W J
authors.initials: WJ
authors.lastname: Peumans
authors.forename: C J
authors.initials: CJ
authors.lastname: De Ranter
mesh_terms: D000045:Acacia / Q000737:chemistry*; D000117:Acetylglucosamine / Q000737:chemistry / Q000378:metabolism*; D001665:Binding Sites; D018360:Crystallography, X-Ray; D006860:Hydrogen Bonding; D037102:Lectins / Q000737:chemistry* / Q000378:metabolism*; D008958:Models, Molecular; D037121:Plant Lectins; D011485:Protein Binding; D011487:Protein Conformation; D055672:Static Electricity; D014867:Water / Q000737:chemistry / Q000378:metabolism
chemical_list: D037102:Lectins; D037121:Plant Lectins; C047720:Robinia lectin; D014867:Water; D000117:Acetylglucosamine