Liver microcirculation after selective denervation.
Microcirculatory disturbances have been related to a decrease in survival after liver transplant. Because innervation is involved in liver hemodynamics regulation, we decided to evaluate microcirculatory hepatic perfusion. Thirty rats were divided into three groups: denervated (DG), hepatic microsurgical denervation; manipulated (MG), hepatic manipulation; control (CG), laparotomy. Hepatic microcirculation was assessed in the median lobe using laser Doppler flowmetry in the following moments: T(0), after laparotomy and T(1), after denervation; and in the following moments after denervation: T(2), 10 minutes, T(3), 20 minutes, T(4), 30 minutes, T(5), 1 hour, T(6), 1.5 hours, and T(7), 2 hours for DG, and in same moments for MG and CG. DG showed a decrease in hepatic perfusion for 20 minutes after denervation, different from MG and CG. After that, there was recovery in hepatic perfusion in MG and DG (Kruskal-Wallis and Friedman tests). Therefore, denervation and manipulation alter hepatic microcirculation, but denervation promotes a more severe decrease than manipulation.
Pedrosa
M E
ME
Operative Technique and Experimental Surgery Division, Department of Surgery, Universidade Federal de São Paulo-Escola Paulista de Medicina, São Paulo, Brazil.
Montero
E F
EF
Nigro
A J
AJ
D000818:Animals; D001783:Blood Flow Velocity / Q000502:physiology; D003714:Denervation; D017078:Laser-Doppler Flowmetry; D008099:Liver / Q000098:blood supply* / Q000294:innervation; D016031:Liver Transplantation / Q000502:physiology; D008297:Male; D008833:Microcirculation / Q000503:physiopathology; D008866:Microsurgery; D051381:Rats