[Enantiomeric high performance liquid chromatographic separation of epinephrine and analogous using chiral mobile phase additives].
The enantiomeric resolution of epinephrine, isoproterenol, ephedrine was studied by reversed phase HPLC. beta-CD, DM-beta-CD, TM-beta-CD were used as chiral mobile phase additives. The effects of the concentration of DM-beta-CD and the concentration of methanol on resolution of D,L-epinephrine were investigated. The results showed: D,L-epinephrine and D,L-isoproterenol can be separated by beta-CD, DM-beta-CD, TM-beta-CD, but D,L-ephedrine can't. Hydrogen bonding plays an important role in the chiral recognition mechanism. In the separation of isoproterenol, the enantioselectivity of TM-beta-CD was better than those of beta-CD and DM-beta-CD. As the concentration of DM-beta-CD changed from 0.04 mmol/L to 1.00 mmol/L, the resolution varied slightly. The experiment of the effect of methanol concentration on Rs showed: when the ratio of methanol/water was 40/60, the Rs reached the maximum. The coefficients of variation of retention time of D-epinephrine and L-epinephrine were 1.3% and 1.4% respectively. It is demonstrated that the chromatographic systems with a dynamically-generated stationary phase with methylated-beta-cyclodextrin are versatile tools for enantiomer separation. This technique leads to column with excellent time stability and good reproducibility of the enantio-selectivity.
Li
H
H
Department of the Science of Analysis and Measurement, Wuhan University, Wuhan, 430072.
Hu
X
X
Xie
Y
Y
D018663:Adrenergic Agents / Q000032:analysis / Q000302:isolation & purification; D000322:Adrenergic Agonists / Q000032:analysis / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D004809:Ephedrine / Q000032:analysis / Q000302:isolation & purification*; D004837:Epinephrine / Q000032:analysis / Q000302:isolation & purification*; D007536:Isomerism; D007545:Isoproterenol / Q000032:analysis / Q000302:isolation & purification*
D018663:Adrenergic Agents; D000322:Adrenergic Agonists; D004809:Ephedrine; D007545:Isoproterenol; D004837:Epinephrine