Isolation and regulation of Sinorhizobium meliloti 1021 loci induced by oxygen limitation.
Eleven Sinorhizobium meliloti 1021 loci whose expression was induced under low oxygen concentrations were identified in a collection of 5,000 strains carrying Tn5-1063 (luxAB) transcriptional reporter gene fusions. The 11 Tn5-1063-tagged loci were cloned and characterized. The dependence of the expression of the tagged loci on the FixL/FixJ oxygen-sensing two-component regulatory system was examined. Three of the loci were found to be dependent upon fixL and fixJ for their expression, while one locus showed a partial dependence. The remaining seven loci showed fixL- and fixJ-independent induction of expression in response to oxygen limitation. This suggests that in S. meliloti, additional regulatory system(s) exist that respond either directly or indirectly to oxygen limitation conditions.
Trzebiatowski
J R
JR
MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824, USA. jparrish@genetics.wayne.edu
Ragatz
D M
DM
de Bruijn
F J
FJ
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D004251:DNA Transposable Elements; D015964:Gene Expression Regulation, Bacterial; D006420:Hemeproteins / Q000235:genetics / Q000378:metabolism; D000071677:Histidine Kinase; D008156:Luciferases / Q000235:genetics; D008163:Luminescent Measurements; D008969:Molecular Sequence Data; D010100:Oxygen / Q000378:metabolism*; D011993:Recombinant Fusion Proteins; D016962:Sinorhizobium meliloti / Q000235:genetics / Q000378:metabolism*; D013559:Symbiosis
D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D006420:Hemeproteins; D011993:Recombinant Fusion Proteins; C069289:FixJ protein, Bacteria; D008156:Luciferases; C067929:FixL protein, Bacteria; D000071677:Histidine Kinase; D010100:Oxygen