Tissue-type plasminogen activator is involved in the process of neuronal death induced by oxygen-glucose deprivation in culture.
Effect of tissue-type plasminogen activator (tPA) on oxygen-glucose deprivation (OGD) was studied in cultured cortical neurons prepared from tPA gene knockout (tPA-KO) and wild-type (Wt) mice. Three hours of OGD induced 45% and 23% of neuronal death in Wt and tPA-KO mice, respectively. Neuronal death in tPA-KO mice was increased to 42% by additional tPA. Six hours of OGD induced 80% and 40% of neuronal death in Wt and tPA-KO mice, respectively, whereas the addition of tPA increased to 62% in tPA-KO mice. These results suggest that tPA is directly involved in the process of neuronal death induced by ischemia-mimic stress without involving vascular or circulatory components.
Nagai
N
N
Department of Physiology, Hamamatsu University School of Medicine, Shizuoka, Japan.
Yamamoto
S
S
Tsuboi
T
T
Ihara
H
H
Urano
T
T
Takada
Y
Y
Terakawa
S
S
Takada
A
A
D000818:Animals; D000831:Animals, Newborn; D016923:Cell Death; D015687:Cell Hypoxia; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology; D005947:Glucose / Q000008:administration & dosage*; D007511:Ischemia; D051379:Mice; D018345:Mice, Knockout; D009474:Neurons / Q000502:physiology*; D010100:Oxygen / Q000008:administration & dosage*; D013997:Time Factors; D010959:Tissue Plasminogen Activator / Q000172:deficiency / Q000502:physiology*
D010959:Tissue Plasminogen Activator; D005947:Glucose; D010100:Oxygen