title: Effect of recombinant human bone morphogenetic protein-2 on bone regeneration and osseointegration of dental implants.
abstract: Recombinant human bone morphogenetic protein-2 (rhBMP-2) induced bone regeneration and osseointegration was evaluated in bony defects created within the hollow chamber of endosseous dental implants in 14 foxhound dogs. Bilateral extractions of mandibular premolars were performed and surgical implantation of 104 hollow cylinder implants followed after 8 weeks of healing. Experimental implants had their hollow chamber filled with 20 microg of rhBMP-2 delivered with a bovine collagen carrier, whereas the control implants had their apical chamber left empty. Dogs were followed for 2, 4, 8 and 12 weeks. Histomorphometric evaluation and immunohistochemical analysis were performed. Minimal bone was regenerated at 2 weeks for both groups. At 4 weeks, bone fill averaged 23.48% for the rhBMP-2 and 5.98% for the control group (P<0.05). At 8 weeks, mean bone fill was 20.94% and 7.75% for the rhBMP-2 and the controls, respectively (P<0.05). At 12 weeks, mean bone fill was 31.39% and 24.31% for the rhBMP-2 and control implants, respectively (P>0.05). Bone-implant contact (BIC) increased for both groups over time and at 8 weeks the rhBMP-2 BIC value was 18.65% and for the control 7.22% (P<0.05). At 12 weeks, the BIC was 43.78% and 21.05% for the rhBMP-2 and the control group, respectively (P<0.05). Immunohistochemical staining for type II collagen was positive only for parts of the collagen carrier and formation of cartilaginous intermediate was not observed in any of the specimens. The results suggest that, in confined defects adjacent to dental implants, rhBMP-2 can induce bone regeneration in close apposition to the implant surface.
authors.affiliation: Private practice, Athens, Greece.
authors.forename: N
authors.initials: N
authors.lastname: Sykaras
authors.forename: R G
authors.initials: RG
authors.lastname: Triplett
authors.forename: M E
authors.initials: ME
authors.lastname: Nunn
authors.forename: A M
authors.initials: AM
authors.lastname: Iacopino
authors.forename: L A
authors.initials: LA
authors.lastname: Opperman
mesh_terms: D000818:Animals; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000494:pharmacology*; D001861:Bone Regeneration / Q000187:drug effects*; D002417:Cattle; D003758:Dental Implantation, Endosseous; D015921:Dental Implants; D004285:Dogs; D006801:Humans; D007150:Immunohistochemistry; D020007:Implants, Experimental; D008297:Male; D008334:Mandible; D016348:Osseointegration / Q000187:drug effects*; D016012:Poisson Distribution; D011994:Recombinant Proteins / Q000494:pharmacology; D012044:Regression Analysis; D016212:Transforming Growth Factor beta
chemical_list: C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D015921:Dental Implants; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; C499469:recombinant human bone morphogenetic protein-2