title: Characterization of the third generation enzyme immunoassay IEA-HIV1/2-III for the detection of anti-HIV specific antibodies in human sera.
abstract: The sensitivity and specificity of the developed anti-HIV1/2 third generation enzyme immunoassay, the IEA-HIV1/2-III, was examined. The test system for the detection of anti-HIV antibodies included peroxidase-conjugated HIV-specific recombinant Gag protein fragments (epitopes of p24 and p17 proteins), Env-1 (epitopes of p41 and p120 proteins), and Env-2 (p36 epitopes). Sensitivity was evaluated with 346 sera from HIV1-seropositive subjects, Anti-HIV1 Low Titer panels no. 10 and PRB-106 and seropositive panel PRB-931 in comparison with other third- and second-generation assays. The IEA-HIV1/2-III assays are characterized with high sensitivity comparable to the other third generation assays and the better sensitivity with respect to the second generation test-kit to determine HIV-specific antibodies in human sera. The specificity was determined using three hundred sixty-seven potentially cross-reactive samples (but negative for anti-HIV1/2). Only one specimen among them was reactive by IEA-HIV1/2-III.
authors.affiliation: Scientific-Production Enterprise Diaproph-Med, Kiev, Ukraine.
authors.forename: G
authors.initials: G
authors.lastname: Rayevskaya
authors.forename: V G
authors.initials: VG
authors.lastname: Pilipenko
authors.forename: L
authors.initials: L
authors.lastname: Tkáciková
authors.forename: N Y
authors.initials: NY
authors.lastname: Spivak
authors.forename: I
authors.initials: I
authors.lastname: Mikula
authors.forename: R M
authors.initials: RM
authors.lastname: Chumak
mesh_terms: D003429:Cross Reactions; D000939:Epitopes; D005260:Female; D015483:HIV Antibodies / Q000097:blood*; D015488:HIV Antigens; D015497:HIV-1 / Q000276:immunology*; D015498:HIV-2 / Q000276:immunology*; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods* / Q000706:statistics & numerical data; D011247:Pregnancy; D012680:Sensitivity and Specificity
chemical_list: D000939:Epitopes; D015483:HIV Antibodies; D015488:HIV Antigens