[Typing of enteric adenoviruses in feces of infants with diarrhea].
OBJECTIVE
To study the infection condition of adenovirus types in infants with diarrhea in Shenzhen.


METHODS
114 fecal samples from diarrhea infants were tested by PCR. The amplified products were analyzed by Taq I and Rsa I endonucleases.


RESULTS
The positive rate of adenovirus DNA in fecal samples was 14% (16/114). The amplified products typing procedure started with Taq I endonuclease to identify the type of adenovirus, 11 cleaved by Taq I yielded two fragments of 191 bp and 110 bp, the positive rate of enteric adenovirus was 9.65% (11/114). Followed by Rsa I cleavage, two amplified products yielded 256 bp and 45 bp fragments (Ad40), the positive rate of Ad40 was 1. 75% (2/114), nine amplified products yielded 211 bp and 90 bp fragments (Ad4l), the positive rate of Ad41 was 7.89%(9/114).


CONCLUSIONS
The positive rates of the adenovirus types 40 and 41 were 9.65% in feces of infants with diarrhea, and that of the other types of adenovirus was 4.35%.
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Department of Microbiology, Shenzhen Health and Anti-epidemic Station, Shenzhen 5l8020, China.
Yang
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D000258:Adenovirus Infections, Human / Q000453:epidemiology*; D000260:Adenoviruses, Human / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D002681:China / Q000453:epidemiology; D004279:DNA, Viral / Q000032:analysis; D003968:Diarrhea, Infantile / Q000821:virology*; D005243:Feces / Q000821:virology*; D006801:Humans; D007223:Infant; D016133:Polymerase Chain Reaction
D004279:DNA, Viral