title: Participation of residue F552 in domain III of the protective antigen in the biological activity of anthrax lethal toxin.
abstract: The protective antigen (PA) component of anthrax toxin translocates the catalytic moieties lethal factor (LF) and edema factor (EF) into the cytosol. The proteolytically activated 63 kDa form of PA (PA63) has the ability to oligomerize and bind LF/EF. PA has four distinct domains performing specialized functions; whereas the function of domains I, II and IV has been well characterized, domain III has no known role in the biological activity of PA. Here we report the role of amino acid residues lining an exposed hydrophobic patch of domain III in the biological activity of PA. The residues Phe552, Phe554, lIe562, Leu566 and lle574 were individually substituted with alanine and the effect was studied. All mutant PA proteins except Phe552Ala were equally active as wild-type PA in exhibiting a toxic phenotype to J774A.1 cells in the presence of LF. Substitution of Ala for Phe552 reduced the ability of PA to intoxicate cells by more than 250-fold. However, Phe552Ala was equally active in receptor binding and susceptibility to trypsin and chymotrypsin as wild-type PA, the activities that have been shown to be essential for the biological activity of PA. This mutated PA protein had a decreased ability to bind LF, oligomerize on cells and to induce release of 86Rb+ from Chinese hamster ovary cells. These results suggest that the residue Phe552 in PA plays an important role in LF binding and oligomerization. Our study provides a basis for further exploration of the biological significance of domain III of PA.
authors.affiliation: Centre for Biochemical Technology, Delhi, India
authors.forename: H
authors.initials: H
authors.lastname: Khanna
authors.forename: P K
authors.initials: PK
authors.lastname: Gupta
authors.forename: A
authors.initials: A
authors.lastname: Singh
authors.forename: R
authors.initials: R
authors.lastname: Chandra
authors.forename: Y
authors.initials: Y
authors.lastname: Singh
mesh_terms: D019943:Amino Acid Substitution; D000818:Animals; D000942:Antigens, Bacterial; D001427:Bacterial Toxins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D001692:Biological Transport; D016466:CHO Cells; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D006224:Cricetinae; D019281:Dimerization; D051379:Mice; D010649:Phenylalanine / Q000737:chemistry; D017434:Protein Structure, Tertiary; D012697:Serine Endopeptidases / Q000378:metabolism
chemical_list: D000942:Antigens, Bacterial; D001427:Bacterial Toxins; C030325:anthrax toxin; D010649:Phenylalanine; D012697:Serine Endopeptidases