Possible presence of common tyvelose-containing glycans in Trichinella L1 larvae and embryonated eggs of several nematodes.
A monoclonal antibody (mAb US4) recognising an epitope containing tyvelose within the T. spiralis L-1 muscle larvae (TSL-1) antigens was tested in western-blot against various antigenic preparations from different stages of the following nematodes: T. spiralis (L1, adult), T. muris (egg, L1, L3, adult), Ascaris suum (egg, adult), Toxocara canis (egg, adult), Anisakis simplex (L3) and Haemochus contortus (egg). Positive reaction was present in antigen preparations from L1 larvae of T. spiralis and T. muris and from embryonated eggs of T. muris, A. seum, T. canis and H. contortus.
Dea-Ayuela
M A
MA
Departamento de Parasitología, Facultad de Farmacia, Universidad Complutense, 28040-Madrid, Spain.
Romarís
F
F
Ubeira
F M
FM
Rama-Iñiguez
S
S
Martínez-Fernández
A R
AR
Bolás
F
F
D000818:Animals; D000909:Antibodies, Helminth; D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D004625:Embryo, Nonmammalian / Q000737:chemistry / Q000276:immunology; D006601:Hexoses / Q000032:analysis*; D007814:Larva / Q000737:chemistry; D009348:Nematoda / Q000737:chemistry* / Q000276:immunology; D011134:Polysaccharides / Q000032:analysis* / Q000737:chemistry* / Q000276:immunology; D014234:Trichinella / Q000737:chemistry* / Q000276:immunology / Q000502:physiology; D017160:Trichinella spiralis / Q000737:chemistry / Q000276:immunology / Q000502:physiology
D000909:Antibodies, Helminth; D000911:Antibodies, Monoclonal; D006601:Hexoses; D011134:Polysaccharides; C009616:tyvelose