title: Postprandial dyslipidemia in men with visceral obesity: an effect of reduced LDL receptor expression?
abstract: Postprandial lipemia after an oral fat challenge was studied in middle-aged men with visceral obesity. The two groups had similar plasma cholesterol levels, but obese subjects had higher levels of plasma triglyceride and reduced amounts of high-density cholesterol. Fasting plasma insulin was fourfold greater in obese subjects because of concomitant insulin resistance, with a calculated HOMA score of 3.1 +/- 0.6 vs. 0.8 +/- 0.2, respectively. Plasma apolipoprotein B(48) (apoB(48)) and retinyl palmitate (RP) after an oral fat challenge were used to monitor chylomicron metabolism. Compared with lean subjects, the fasting concentration of apoB(48) was more than twofold greater in obese individuals, suggestive of an accumulation of posthydrolyzed particles. After the oral lipid load, the incremental areas under the apoB(48) and RP curves (IAUC) were both significantly greater in obese subjects (apoB(48): 97 +/- 17 vs. 44 +/- 12 microg.ml(-1). h; RP: 3,120 +/- 511 vs. 1,308 +/- 177 U. ml(-1). h, respectively). A delay in the conversion of chylomicrons to remnants probably contributed to postprandial dyslipidemia in viscerally obese subjects. The triglyceride IAUC was 68% greater in obese subjects (4.7 +/- 0.6 vs. 2.8 +/- 0.8 mM. h, P < 0.06). Moreover, peak postprandial triglyceride was delayed by approximately 2 h in obese subjects. The reduction in triglyceride lipolysis in vivo did not appear to reflect changes in hydrolytic enzyme activities. Postheparin plasma lipase rates were found to be similar for lean and obese subjects. In this study, low-density lipoprotein (LDL) receptor expression on monunuclear cells was used as a surrogate marker of hepatic activity. We found that, in obese subjects, the binding of LDL was reduced by one-half compared with lean controls (70.9 +/- 15.07 vs. 38.9 +/- 4.6 ng LDL bound/microg cell protein, P = 0.02). Because the LDL receptor is involved in the removal of proatherogenic chylomicron remnants, we suggest that the hepatic clearance of these particles might be compromised in insulin-resistant obese subjects. Premature and accelerated atherogenesis in viscerally obese, insulin-resistant subjects may in part reflect delayed clearance of postprandial lipoprotein remnants.
authors.affiliation: Department of Nutrition, Dietetics and Food Sciences, School of Public Health, Curtin University of Technology, Perth 6845, Australia. J.Mamo@curtin.edu.au
authors.forename: J C
authors.initials: JC
authors.lastname: Mamo
authors.forename: G F
authors.initials: GF
authors.lastname: Watts
authors.forename: P H
authors.initials: PH
authors.lastname: Barrett
authors.forename: D
authors.initials: D
authors.lastname: Smith
authors.forename: A P
authors.initials: AP
authors.lastname: James
authors.forename: S
authors.initials: S
authors.lastname: Pal
mesh_terms: D053283:Apolipoprotein B-48; D001055:Apolipoproteins B / Q000097:blood; D001161:Arteriosclerosis / Q000209:etiology; D001824:Body Constitution; D015992:Body Mass Index; D002784:Cholesterol / Q000097:blood; D008076:Cholesterol, HDL / Q000097:blood; D002914:Chylomicrons / Q000097:blood; D004041:Dietary Fats / Q000008:administration & dosage; D004224:Diterpenes; D005502:Food; D006801:Humans; D006949:Hyperlipidemias / Q000209:etiology*; D007328:Insulin / Q000097:blood; D007333:Insulin Resistance; D007700:Kinetics; D008049:Lipase / Q000097:blood; D008077:Lipoproteins, LDL / Q000097:blood; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000150:complications* / Q000378:metabolism; D011973:Receptors, LDL / Q000097:blood*; D000084562:Retinyl Esters; D014280:Triglycerides / Q000097:blood; D014781:Viscera; D014801:Vitamin A / Q000031:analogs & derivatives / Q000097:blood
chemical_list: D053283:Apolipoprotein B-48; D001055:Apolipoproteins B; D008076:Cholesterol, HDL; D002914:Chylomicrons; D004041:Dietary Fats; D004224:Diterpenes; D007328:Insulin; D008077:Lipoproteins, LDL; D011973:Receptors, LDL; D000084562:Retinyl Esters; D014280:Triglycerides; D014801:Vitamin A; C014794:retinol palmitate; D002784:Cholesterol; D008049:Lipase