Inhibition of TPA-induced cyclooxygenase-2 expression and skin inflammation in mice by wogonin, a plant flavone from Scutellaria radix.
Wogonin (5,7-dihydroxy-8-methoxyflavone), isolated from Scutellaria radix, was previously reported to inhibit the expression and activity of the enzyme cyclooxygenase-2 in lipopolysaccharide (LPS)-stimulated cells of a mouse macrophage cell line, RAW 264.7. Here, in order to find in vivo effects, inhibition by wogonin of 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced cyclooxygenase-2 expression and anti-inflammatory activity in vivo were investigated. When applied topically to the dorsal skin of mice, wogonin at doses of 50-200 microg/site/treatment (total of five treatments in 3 days) inhibited cyclooxygenase-2 expression and prostaglandin E2 production induced by multiple treatments with TPA. At 200 microg/site/treatment, wogonin caused a 55.3% reduction of prostaglandin E2 production on the dorsal skin compared with an increased production in the TPA-treated control group. The same compound significantly inhibited mouse ear edema induced by TPA in both preventive (58.1% inhibition) as well as curative treatment (31.3% inhibition) schedules at 200 microg/ear/treatment. Inhibition of neutrophil infiltration was also observed. Therefore, wogonin may be beneficial for cyclooxygenase-2-related skin disorders.
Park
B K
BK
College of Pharmacy, Kangwon National University, 200-701, Chunchon, South Korea.
Heo
M Y
MY
Park
H
H
Kim
H P
HP
D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use*; D051546:Cyclooxygenase 2; D015232:Dinoprostone / Q000096:biosynthesis; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use; D004487:Edema / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D044950:Flavanones; D047309:Flavones; D005419:Flavonoids / Q000627:therapeutic use*; D015870:Gene Expression / Q000187:drug effects; D007527:Isoenzymes / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D019684:Magnoliopsida / Q000737:chemistry; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010936:Plant Extracts / Q000737:chemistry; D011451:Prostaglandin-Endoperoxide Synthases / Q000096:biosynthesis; D012871:Skin Diseases / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D044950:Flavanones; D047309:Flavones; D005419:Flavonoids; D007527:Isoenzymes; D010936:Plant Extracts; D051546:Cyclooxygenase 2; D011451:Prostaglandin-Endoperoxide Synthases; D015232:Dinoprostone; D013755:Tetradecanoylphorbol Acetate; C085514:wogonin; C043562:flavone