[Establishment and application of Z-HL16C cell line].
OBJECTIVE
Human embryonic lung fibroblasts at 8th passage was presented to us by the Institute of Virology. At its 16th passage,under the culture condition of our laboratory, the lung fibroblasts changed into epithelial like cells. We attempted to establish a cell line and applied it for virus isolation.


METHODS
The cells were continuously passaged, cloned, the cell morphology, growth capacity and chromosome number were determined.


RESULTS
The cell size appeared uniform, cell boundary was distinct, the cell recovery rate after frozen storing was above 90%. The cell replicated permanently and now it has been passaged 136 times. The chromosome number has changed from 46 to 110. We named this cell line the ZHL16C. It proved to be sensitive to these viruses tested: enteroviruses (Polio, Cox, Echo), influenza viruses, parainfluenza viruses, adenoviruses, measles virus, herpes simplex and herpes zoster viruses, cytomegalovirus, rubella virus and respiratory syncytial virus. When using Z-HL16C cell to isolate virus from 29 adenovirus swab samples collected from 29 soldiers with epidemic high fever in a new military, adenovirus type 3,7 coxsackie virus types B1, B5, B5, B5, B5 were isolated.


CONCLUSIONS
The cell line HL16C has been stably established, it has a broad spectrum in sensitivity for viruses.
Zhao
S
S
Phargen Biotech Application Research Institute, Guangdong Shantou S. E. Z, 515041, China.
Liu
Z
Z
Xie
R
R
D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D002999:Clone Cells; D005347:Fibroblasts / Q000166:cytology* / Q000821:virology; D006801:Humans; D008168:Lung / Q000166:cytology* / Q000196:embryology