Identification of a novel Rab11/25 binding domain present in Eferin and Rip proteins.
Rab11, a low molecular weight GTP-binding protein, has been shown to play a key role in a variety of cellular processes, including endosomal recycling, phagocytosis, and transport of secretory proteins from the trans-Golgi network. In this study we have described a novel Rab11 effector, EF-hands-containing Rab11-interacting protein (Eferin). In addition, we have identified a 20-amino acid domain that is present at the C terminus of Eferin and other Rab11/25-interacting proteins, such as Rip11 and nRip11. Using biochemical techniques we have demonstrated that this domain is necessary and sufficient for Rab11 binding in vitro and that it is required for localization of Rab11 effector proteins in vivo. The data suggest that various Rab effectors compete with each other for binding to Rab11/25 possibly accounting for the diversity of Rab11 functions.
Prekeris
R
R
Howard Hughes Medical Institute, Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, California 94305-5428, USA.
Davies
J M
JM
Scheller
R H
RH
D000595:Amino Acid Sequence; D000818:Animals; D002352:Carrier Proteins / Q000737:chemistry* / Q000378:metabolism; D002460:Cell Line; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D011992:Endosomes / Q000737:chemistry; D005982:Glutathione Transferase / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000378:metabolism; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D010587:Phagocytosis; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry*; D053422:Receptor-Interacting Protein Serine-Threonine Kinases; D017386:Sequence Homology, Amino Acid; D020798:Two-Hybrid System Techniques; D020691:rab GTP-Binding Proteins / Q000737:chemistry*; D021601:trans-Golgi Network; D000099193:rab11 GTP-Binding Proteins
D002352:Carrier Proteins; D005982:Glutathione Transferase; D006160:Guanosine Triphosphate; D011506:Proteins; D053422:Receptor-Interacting Protein Serine-Threonine Kinases; D020691:rab GTP-Binding Proteins; D000099193:rab11 GTP-Binding Proteins; C439063:RAB11FIP3 protein, human; C506249:RIPK1 protein, human