Antigen recognition by IgG4 antibodies in human trichinellosis.
The antibody isotype response to Trichinella spiralis excretory/secretory (ES) products of muscle larva was examined using sera from patients with confirmed trichinellosis. Using Western blots we identify components of the ES antigen that are recognized by IgM and IgG antibodies. A 45 kDa component was strongly recognized by different antibody classes and subclasses. We observed a 45 kDa-specific IgG4 response that was detected exclusively using sera of patients with trichinellosis and not of patients with echinococcosis, filariasis, cysticercosis, ascariasis, strongyloidiasis or toxocariasis. These results are relevant for the diagnosis of human trichinellosis.
Pinelli
E
E
Diagnostic Laboratory for Infectious Diseases and Perinatal Screening, National Institute of Public Health and the Environment (RIVM), Bilthaven, The Netherlands. elena.pinelli@rivm.nl
Van Der Lugt
G
G
Homan
W
W
Van Der Giessen
J
J
Kortbeek
L M
LM
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000937:Antigen-Antibody Reactions; D000947:Antigens, Helminth / Q000276:immunology*; D015153:Blotting, Western; D004196:Disease Outbreaks; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D007132:Immunoglobulin Isotypes / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D007814:Larva; D011044:Poland / Q000453:epidemiology; D017160:Trichinella spiralis / Q000276:immunology*; D014235:Trichinellosis / Q000097:blood / Q000453:epidemiology / Q000276:immunology*
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D007074:Immunoglobulin G; D007132:Immunoglobulin Isotypes; D007075:Immunoglobulin M