[Effect of HH07A, A derivative of hainanensine, on DNA polymerase I].
The effect of HH07A on DNA polymerase I in a cell free system was studied. The results showed that HH07A strongly inhibited the DNA synthesis catalyzed by DNA polymerase I in a dose dependent manner. Further results indicated that the DNA synthesis decreased significantly after DNA polymerase I was preincubated with HH07A. However, no change was found when DNA template was preincubated with HH07A. These results suggest that the inhibitory action of HH07A appeared to be on the enzyme molecules rather than on the DNA template.
Ye
Y M
YM
Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050.
Xu
C X
CX
D002474:Cell-Free System; D004247:DNA / Q000096:biosynthesis*; D004256:DNA Polymerase I / Q000187:drug effects* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D006248:Harringtonines / Q000494:pharmacology*
C095422:HH07A; D006248:Harringtonines; D004247:DNA; D004256:DNA Polymerase I