An improved method for the determination of betaine in Echinacea products.
A rapid and sensitive HPLC method for the separation and quantification of betaine in Echinacea products has been developed. Strong cation-exchange (SCX) material was used as stationary phase, and a mixture of methanol and 50 mM choline buffer (pH 3.5) as mobile phase. After formation of the bromophenacyl derivative, betaine was detected at 254 nm with a detection limit of 0.2 microgram/ml. The method was successfully used to analyze several Echinacea market products, and significant variations in their betaine content from 0.04 to 0.64% were observed.
Ganzera
M
M
Institute of Pharmaceutical Sciences, National Center for Natural Products Research, University of Mississippi, USA.
Pujol
P
P
Walker
L
L
Khan
I A
IA
D001622:Betaine / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D020900:Echinacea / Q000737:chemistry*; D010946:Plants, Medicinal; D012015:Reference Standards; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
D001622:Betaine