title: Predominant human rotavirus genotype G1P[8] infection in infants and children in Bangkok, Thailand.
abstract: Human rotavirus is the major etiologic agent of infantile diarrhea on a worldwide scale. In this study, rotaviruses were detected by reverse-transcription PCR in 42 of 83 stool specimens from children below the age of 3 years with acute diarrhea in Bangkok, Thailand, between November 1998 and August 1999. G and P types of all samples were characterized by restriction endonuclease analysis (REA) and multiplex PCR typing assay, respectively. Strain G1P[8] (76.1%) was the predominant type, followed by G1P[6] (2.4%). Strain G1 combined with mixed P[8]/P[6] was identified in 2 specimens (4.8%) and 7 untypeable G strains (16.7%) were observed. This information on the circulating G and P combinations should be useful for understanding the epidemiology of human rotavirus in Bangkok, Thailand.
authors.affiliation: Department of Microbiology, Faculty of Medicine, Chulalongkorn University & Hospital, Bangkok, Thailand.
authors.forename: S
authors.initials: S
authors.lastname: Noppornpanth
authors.forename: A
authors.initials: A
authors.lastname: Theamboonlers
authors.forename: Y
authors.initials: Y
authors.lastname: Poovorawan
mesh_terms: D000208:Acute Disease; D001483:Base Sequence; D002669:Child Welfare; D002675:Child, Preschool; D004262:DNA Restriction Enzymes / Q000032:analysis; D005243:Feces / Q000821:virology; D005759:Gastroenteritis / Q000145:classification / Q000235:genetics; D005819:Genetic Markers / Q000235:genetics; D005838:Genotype; D006801:Humans; D007223:Infant; D007229:Infant Welfare; D007231:Infant, Newborn; D008969:Molecular Sequence Data; D000091402:Prohibitins; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012400:Rotavirus Infections / Q000145:classification / Q000453:epidemiology / Q000235:genetics*; D012703:Serotyping; D013785:Thailand / Q000453:epidemiology
chemical_list: D005819:Genetic Markers; C000717331:PHB2 protein, human; D000091402:Prohibitins; D004262:DNA Restriction Enzymes