Detection and molecular characterization of the zot gene in Vibrio cholerae and V. alginolyticus isolates.
A total of 11 Vibrio cholerae isolates from 1996-1998 outbreaks in Malaysia and 4 V. alginolyticus were analyzed. Isolates were characterized by polymerase chain reaction (PCR) and Southern hybridization for the presence of the gene encoding zonula occludens toxin (zot). Screening of zot gene by PCR revealed the presence of this gene in V. cholerae and V. alginolyticus. The zot gene from one V. cholerae Ogawa isolate that was cloned in a pCR 2.1 TOPO vector was sequenced. The sequences obtained were 99% homologous to the zot gene sequence from the Gene Bank.
Raja
N
N
Department of Clinical Laboratory Sciences, Faculty of Medicine and Heath Sciences, University Putra Malaysia, Serdang, Selangor.
Shamsudin
M N
MN
Somarny
W
W
Rosli
R
R
Rahim
R A
RA
Radu
S
S
D001483:Base Sequence; D015139:Blotting, Southern; D002772:Cholera Toxin / Q000235:genetics*; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D004731:Endotoxins; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D014733:Vibrio / Q000235:genetics*; D014734:Vibrio cholerae / Q000235:genetics*
D004269:DNA, Bacterial; D004731:Endotoxins; C077728:zonula occludens toxin, Vibrio cholerae; D002772:Cholera Toxin