[Inhibitive effect of bcl-2 antisense oligodeoxynucleotide on Bcl-2 protein synthesis and cell proliferation in human laryngocarcinoma Hep-2 cells].
OBJECTIVE
To evaluate the role of bcl-2 antisense oligodeoxynucleotide in the gene treatment of human laryngocarcinoma.


METHODS
An antisense oligodeoxynucleotide complementary to the initiation coden and the next four codens of bcl-2 mRNA was synthesized with an automatic DNA synthesizer. It was used to treat cultured Hep-2 cells to observe its effect on cell proliferation. In situ hybridization method and immunohistochemical technology were adopted to detect bcl-2 mRNA and protein and explore the effect of bcl-2 antisense oligodeoxynucleotide fragment on bcl-2 gene expression and protein synthesis and inhibition of cell proliferation.


RESULTS
Bcl-2 antisense oligodeoxynucleotide had an obvious effect on bcl-2 mRNA, but inhibited Bcl-2 protein synthesis significantly, the inhibitive rate had positive correlation with concentration of antisense oligodeoxynucleotide and time of action. Twenty mumol/L of antisense oligodeoxynucleotide could inhibit cell proliferation effectively.


CONCLUSIONS
It is suggested that bcl-2 antisense oligodeoxynucleotide might specifically inhibit bcl-2 protein synthesis and cell proliferation in Hep-2 cells at translation level.
Jing
H
H
Department of Otorhinolaryngology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032.
Huang
W
W
Qiu
J
J
D002455:Cell Division / Q000187:drug effects; D019254:Genes, bcl-2; D006801:Humans; D007822:Laryngeal Neoplasms / Q000378:metabolism / Q000473:pathology*; D020319:Oligodeoxyribonucleotides, Antisense / Q000494:pharmacology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D014407:Tumor Cells, Cultured
D020319:Oligodeoxyribonucleotides, Antisense; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger