[Isolation, identification and certain characteristics of a Hantaan virus proposed as candidate virus for killed vaccine].
OBJECTIVE
Isolation and identification of a Hantaan virus from HFRS patient's serum for killed vaccine development.


METHODS
This virus temporally called FT14 detected by IFAT, RT-PCR. Partial sequence of M segment was determined by Sanger' a dideoxy chain termination method.


RESULTS
The antigenicity and PCR amplification of the newly isolated virus FT14 proved that it belongs to virus of HTN type. Antiserum to the virus can neutralize many strains of Hantaan virus from different areas. The partial sequence of M segment of FT14, as compared with other HTN strains, showed great differences among nucleotide sequences, while the deduced amino acid sequence is comparatively conservative. The titer of the virus reached its peak at 9 days post inoculation in vitro, then the peak titer could last for a long time.


CONCLUSIONS
This virus strain FT14 would be a good candidate virus for killed vaccine development.
Xie
Y
Y
Institute of Virology, Chinese Academy of Preventive Medicine, Beijing 100052, China.
Hang
C
C
Wang
H
H
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002522:Chlorocebus aethiops; D018063:Hantaan virus / Q000235:genetics / Q000276:immunology*; D006480:Hemorrhagic Fever with Renal Syndrome / Q000821:virology*; D006801:Humans; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000235:genetics; D015164:Vaccines, Inactivated; D014614:Vaccines, Synthetic; D014709:Vero Cells; D014765:Viral Vaccines
D012367:RNA, Viral; D015164:Vaccines, Inactivated; D014614:Vaccines, Synthetic; D014765:Viral Vaccines