title: DsbC activation by the N-terminal domain of DsbD.
abstract: The correct formation of disulfide bonds in the periplasm of Escherichia coli involves Dsb proteins, including two related periplasmic disulfide-bond isomerases, DsbC and DsbG. DsbD is a membrane protein required to maintain the functional oxidation state of DsbC and DsbG. In this work, purified proteins were used to investigate the interaction between DsbD and DsbC. A 131-residue N-terminal fragment of DsbD (DsbDalpha) was expressed and purified and shown to form a functional folded domain. Gel filtration results indicate that DsbDalpha is monomeric. DsbDalpha was shown to interact directly with and to reduce the DsbC dimer, thus increasing the isomerase activity of DsbC. The DsbC-DsbDalpha complex was characterized, and formation of the complex was shown to require the N-terminal dimerization domain of DsbC. These results demonstrate that DsbD interacts directly with full-length DsbC and imply that no other periplasmic components are required to maintain DsbC in the functional reduced state.
authors.affiliation: School of Biological Sciences, University of Auckland, New Zealand.
authors.forename: D
authors.initials: D
authors.lastname: Goldstone
authors.forename: P W
authors.initials: PW
authors.lastname: Haebel
authors.forename: F
authors.initials: F
authors.lastname: Katzen
authors.forename: M W
authors.initials: MW
authors.lastname: Bader
authors.forename: J C
authors.initials: JC
authors.lastname: Bardwell
authors.forename: J
authors.initials: J
authors.lastname: Beckwith
authors.forename: P
authors.initials: P
authors.lastname: Metcalf
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism*; D003545:Cysteine / Q000737:chemistry; D019281:Dimerization; D004926:Escherichia coli / Q000201:enzymology*; D046911:Macromolecular Substances; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism*; D008958:Models, Molecular; D010084:Oxidation-Reduction; D011487:Protein Conformation; D019704:Protein Disulfide-Isomerases / Q000737:chemistry / Q000378:metabolism*; D017434:Protein Structure, Tertiary
chemical_list: D001426:Bacterial Proteins; D046911:Macromolecular Substances; D008565:Membrane Proteins; D019704:Protein Disulfide-Isomerases; D003545:Cysteine