[Genotyping of HIV resistant alleles in indigenous Tibetan ethnic group of China: low frequency of CCR5triangle32 and high frequency of CCR2b-64I alleles].
OBJECTIVE
To investigate the allelic polymorphism of CCR5triangle32, CCR5m303, CCR2b-64I and SDF1-3'A in Tibetan population in Lasa area of China.


METHODS
The genomic DNA samples from 330 Tibetan subjects' whole blood samples were purified by use of QIAgen Blood Kit and identified by PCR or PCR-RFLP analyses.


RESULTS
The mutation frequencies of CCR5triangle32 and CCR5m303 alleles were lower than 0.15%, and those of CCR2 b-64I and SDF1-3'A alleles were 29.42% and 19.24% respectively in the study samples. The allelic polymorphisms of the four alleles of Tibetan population were similar to those of Chinese Han population. Genotype distribution of the four alleles was in accordance with Hardy-Weinberg equilibrium. The above results suggest that Tibetan population may be relatively susceptive to HIV-1.


CONCLUSIONS
The Chinese Tibetan may have a lower frequency of CCR5triangle32 and a higher frequency of CCR2b-64I allele, compared with Caucasian.
Hong
W
W
Institution of Infectious Disease of 302 Hospital, Beijing 100039 P.R.China.
Wang
F
F
Jin
L
L
Xing
L
L
Liu
M
M
Du
Q
Q
Lei
Z
Z
Li
J
J
D000293:Adolescent; D000483:Alleles; D044466:Asian People / Q000235:genetics*; D002681:China / Q000208:ethnology; D005006:Ethnicity; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D015658:HIV Infections / Q000235:genetics*; D006801:Humans; D007113:Immunity, Innate / Q000235:genetics*; D008297:Male; D011110:Polymorphism, Genetic; D018609:Tibet / Q000208:ethnology