title: CENP-H, a constitutive centromere component, is required for centromere targeting of CENP-C in vertebrate cells.
abstract: CENP-H has recently been discovered as a constitutive component of the centromere that co-localizes with CENP-A and CENP-C throughout the cell cycle. The precise function, however, remains poorly understood. We examined the role of CENP-H in centromere function and assembly by generating a conditional loss-of-function mutant in the chicken DT40 cell line. In the absence of CENP-H, cell cycle arrest at metaphase, consistent with loss of centromere function, was observed. Immunocytochemical analysis of the CENP-H-deficient cells demonstrated that CENP-H is necessary for CENP-C, but not CENP-A, localization to the centromere. These findings indicate that centromere assembly in vertebrate cells proceeds in a hierarchical manner in which localization of the centromere-specific histone CENP-A is an early event that occurs independently of CENP-C and CENP-H.
authors.affiliation: National Institute of Genetics and Graduate University for Advanced Studies, Mishima, Shizuoka 411-8540, Japan. tfukagaw@lab.nig.ac.jp
authors.forename: T
authors.initials: T
authors.lastname: Fukagawa
authors.forename: Y
authors.initials: Y
authors.lastname: Mikami
authors.forename: A
authors.initials: A
authors.lastname: Nishihashi
authors.forename: V
authors.initials: V
authors.lastname: Regnier
authors.forename: T
authors.initials: T
authors.lastname: Haraguchi
authors.forename: Y
authors.initials: Y
authors.lastname: Hiraoka
authors.forename: N
authors.initials: N
authors.lastname: Sugata
authors.forename: K
authors.initials: K
authors.lastname: Todokoro
authors.forename: W
authors.initials: W
authors.lastname: Brown
authors.forename: T
authors.initials: T
authors.lastname: Ikemura
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001324:Autoantigens; D002453:Cell Cycle; D002460:Cell Line; D002503:Centromere / Q000378:metabolism / Q000502:physiology*; D000076247:Centromere Protein A; D002645:Chickens; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics / Q000378:metabolism* / Q000502:physiology*; D002869:Chromosome Aberrations; D002874:Chromosome Mapping; D020090:Chromosome Segregation; D006801:Humans; D008677:Metaphase; D008969:Molecular Sequence Data; D016296:Mutagenesis; D017386:Sequence Homology, Amino Acid; D013997:Time Factors
chemical_list: D001324:Autoantigens; C000619700:CENPA protein, human; C121933:CENPH protein, human; D000076247:Centromere Protein A; D002868:Chromosomal Proteins, Non-Histone; C060905:centromere protein C