title: Sodium current in NG108-15 cell inhibited by scorpion toxin BmKAS-1 and restored by its specific monoclonal antibodies.
abstract: Effect on Na+ current of BmKAS-1, a novel polypeptide purified from the venom of Chinese scorpion Buthus martensi Karsch (BmK), has been investigated in differentiated NG108-15 cells by using patch-clamp whole-cell recording. Neutralizing effect of four monoclonal antibodies of BmKAS-1 (mAb #2, #3, #4, and #5) has also been observed. The results showed that Na+ current was irreversibly inhibited by BmKAS-1 and the inhibitory effect was abolished by mAb #2 and #5, but not by mAb #3 and #4.
authors.affiliation: Institute of Physiology, Chinese Academy of Sciences, People's Republic of China.
authors.forename: Y
authors.initials: Y
authors.lastname: Wu
authors.forename: Y H
authors.initials: YH
authors.lastname: Ji
authors.forename: Y L
authors.initials: YL
authors.lastname: Shi
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D018929:Cell Culture Techniques; D004594:Electrophysiology; D010455:Peptides; D012604:Scorpion Venoms / Q000276:immunology / Q000494:pharmacology*; D015222:Sodium Channels / Q000187:drug effects*
chemical_list: D000911:Antibodies, Monoclonal; C119408:KAS-1 toxin, Buthus martensi; D010455:Peptides; D012604:Scorpion Venoms; D015222:Sodium Channels