[Selective inhibition of HSV-1 DNA and protein synthesis by antiviral antibiotic 17997].
OBJECTIVE
To study the effects of 17997 on the HSV-1 DNA and protein synthesis.


METHODS
DNA synthesis was determined by isopycnic separation of cellular and viral DNA in CsCl gradients centrifugation. Protein synthesis was determined by SDS-PAGE and Western blot.


RESULTS
The inhibition rates of 0.5 micromol/L, 1.0 micromol/L, 2.0 micromol/L and 4.0 micromol/L of 17997 on HSV-1 DNA synthesis were 63.1%,74. 1%, 93.9% and 100%, respectively. 4.0 micromol/L of 17997 did not inhibit VERO cell DNA synthesis. The above concentrations of 17997 did not show any inhibitory effects on cellular protein synthesis, but selectively inhibited on viral protein synthesis.


CONCLUSIONS
17997 selectively displayed inhibitive effects on HSV-1 DNA and protein synthesis.
Chen
E
E
Institute of Medicinal Biotechnology, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100050, China.
Yang
M
M
Tao
P
P
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000998:Antiviral Agents / Q000494:pharmacology*; D002522:Chlorocebus aethiops; D004279:DNA, Viral / Q000187:drug effects*; D018259:Herpesvirus 1, Human / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D014709:Vero Cells; D014764:Viral Proteins / Q000096:biosynthesis*
D000900:Anti-Bacterial Agents; D000998:Antiviral Agents; D004279:DNA, Viral; D014764:Viral Proteins