[Effect of enkephalin on glial neurotrophic function related to the reduction of nitric oxide production].
The effect of methionine-enkephalin (ME) on glial neurotrophic function was studied in rat cortical glial-neuronal coculture. The results showed that ME-treated glia enhanced neuronal survival by 28% (P < 0.05), and increased total neurite length per neuron by 11% (P < 0.05), while the expression of GAP-43 mRNA by 26% (P < 0.05). The effect of ME on NO production of glia was also studied in rat cortical glial culture. The result showed that different concentrations of ME (10(-12), 10(-10), 10(-8), 10(-6) mol/L) inhibited glial NO production. All the above show that the increase of glial neurotrophic ability by ME may be due to the inhibition of glial NO production.
Wei
G W
GW
State Key Laboratory of Medical Neurobiology, Department of Neurobiology, Shanghai Medical University, Shanghai 200032.
Du
L N
LN
Zhu
C Q
CQ
Tang
C R
CR
Cao
X D
XD
Wu
G C
GC
D000818:Animals; D000831:Animals, Newborn; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology; D004744:Enkephalin, Methionine / Q000494:pharmacology* / Q000502:physiology; D019922:GAP-43 Protein / Q000096:biosynthesis* / Q000235:genetics; D009414:Nerve Growth Factors / Q000494:pharmacology* / Q000502:physiology; D009457:Neuroglia / Q000502:physiology*; D009569:Nitric Oxide / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley
D019922:GAP-43 Protein; D009414:Nerve Growth Factors; D012333:RNA, Messenger; D009569:Nitric Oxide; D004744:Enkephalin, Methionine