Interleukin-10 gene promoter polymorphism in Japanese patients with adult and early-onset periodontitis.
BACKGROUND
IL-10 is an anti-inflammatory cytokine, which may modulate disease expression in chronic inflammatory periodontal disease. 3 dimorphic polymorphisms within the IL-10 gene promoter have recently been identified and appear to influence regulation of its expression.


OBJECTIVE
The aim of the present study was to investigate whether the promoter polymorphisms are associated with adult periodontitis (AP) and generalized early-onset periodontitis (G-EOP).


METHODS
Genomic DNA was obtained from 34 AP patients, 18 G-EOP patients and 52 controls. The promoter region between -506 and -1140 was amplified by polymerase chain reaction, and polymorphisms were detected by nucleotide sequencing.


RESULTS
The haplotype frequencies in Japanese were quite different from those of Caucasian and were even slightly different from those of southern Chinese with systemic lupus erythematosus. We found no significant difference in allele or haplotype frequencies between patients and controls.


CONCLUSIONS
IL-10 production may be regulated within the complex cytokine network in chronic inflammatory periodontal disease, rather than the gene polymorphisms.
Yamazaki
K
K
Department of Periodontology, Faculty of Dentistry, Niigata University, Niigata, Japan. kaz@dent.niigata-u.ac.jp
Tabeta
K
K
Nakajima
T
T
Ohsawa
Y
Y
Ueki
K
K
Itoh
H
H
Yoshie
H
H
D000328:Adult; D000483:Alleles; D016022:Case-Control Studies; D002681:China / Q000208:ethnology; D004252:DNA Mutational Analysis; D005260:Female; D006239:Haplotypes / Q000235:genetics*; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics*; D007564:Japan / Q000208:ethnology; D008297:Male; D010518:Periodontitis / Q000235:genetics*; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic; D044465:White People / Q000235:genetics
D016753:Interleukin-10