In vitro culture of human epithelial cells on a modified xenogenic collagen support.
Human epithelial cells (HeLa, HaCaT, NHK) were cultured in vitro on chemically modified collagen membranes. Adhesion to the support was measured by estimation of the percentage of adhering 51Cr-labeled cells. Proliferation was estimated with the XTT test. Morphological observations of cells growing on HCl-treated collagen were performed using histological and electron microscopic techniques. HCl and trypsin-modified xenogenic collagen was found to be a good support for human cells in vitro. EDTA-incubated collagen enhanced neither adhesion nor proliferation. The best adhesion and proliferation were found on HCl-treated collagen, depending, however, on the kind of cells.
Lesiak-Cyganowska
E
E
Department of Transplantology and Central Tissue Bank, Centre of Biostructure Research, Warsaw Medical University, Poland. elesiak@ib.amwaw.edu.pl
Sladowski
D
D
Jankowska
E
E
Komender
J
J
D002448:Cell Adhesion; D002455:Cell Division; D002478:Cells, Cultured; D003094:Collagen / Q000494:pharmacology*; D004492:Edetic Acid / Q000494:pharmacology; D004847:Epithelial Cells / Q000166:cytology / Q000502:physiology; D006801:Humans; D006851:Hydrochloric Acid / Q000494:pharmacology; D014357:Trypsin / Q000494:pharmacology
D003094:Collagen; D004492:Edetic Acid; D014357:Trypsin; D006851:Hydrochloric Acid