Selective cytotoxicity of MIA Pa Ca-2 conditioned medium to acinar cells: a novel approach to reduce acinar cell contaminants in isolated islet preparations from BALB/c mice.
Contamination of acinar cells in islet preparations has been shown to affect islet viability, functionality and yield adversely. Therefore, a strategy which would reduce acinar contamination in islet preparations is much sought. We here demonstrate selective cytotoxicity of conditioned medium (CM) of MIA Pa Ca-2 (human pancreatic carcinoma) cells to acinar cells and suitability of this approach as a simple method of obtaining a pure islet population without affecting their viability and yield. When isolated, islet preparations from BALB/c mice were exposed to conditioned media of MIA Pa Ca-2, acinar cells underwent extensive degeneration to yield 80-85% pure islet population, and islets showed comparable viability to controls not exposed to conditioned media. They also maintained their normal morphology, as assessed by digital image analysis. Islets treated with a conditioned medium also preserved in vitro insulin secretion. Flow cytometric analysis of acinar cells treated with conditioned media revealed accelerated DNA damage (45%), compared to controls (22%). Results emphasize the role of factors secreted by MIA Pa Ca-2 cells in inducing selective toxicity to acinar cells.
Kurup
S
S
Tissue Engineering and Banking Laboratory, National Center for Cell Science, Pune, India.
Risbud
M V
MV
Bhonde
R R
RR
D000818:Animals; D002470:Cell Survival; D017077:Culture Media, Conditioned / Q000506:poisoning*; D004249:DNA Damage; D007515:Islets of Langerhans / Q000166:cytology* / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D010179:Pancreas / Q000166:cytology* / Q000187:drug effects*; D012016:Reference Values; D013048:Specimen Handling / Q000379:methods*; D014407:Tumor Cells, Cultured
D017077:Culture Media, Conditioned