title: Modulatory effects of neonatal exposure to TCDD, or a mixture of PCBs, p,p'-DDT, and p-p'-DDE, on methylnitrosourea-induced mammary tumor development in the rat.
abstract: The role of organochlorine (OC) exposure in the etiology of breast cancer remains controversial. Thus, our objective was to determine whether the most abundant and toxic OCs found in human milk could, when ingested during the neonatal period, modulate the development of mammary tumors in the rat. We prepared a mixture composed of p,p'-dichlorodiphenyltrichloroethane (DDT), its major metabolite, p,p'-dichlorodiphenyldichloroethene (DDE), and 19 polychlorinated biphenyls (PCB) based on their concentrations found in the milk of Canadian women. Neonate rats at 1, 5, 10, 15, and 20 days of age were gavaged with this mixture, at 10, 100, and 1,000 times the amount that a human baby would consume. An additional group received 2.5 microg 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)/kg body weight (bw) by gavage at 18 days of age, instead of the mixture. On day 21, all treatment groups, except for a control group and a 1,000-mix group, received a single intraperitoneal injection of methylnitrosourea (MNU, 30 mg/kg bw), the initiator of the carcinogenic process. The average number of rats per treatment group was 33. Rats were sacrificed when their tumors reached 1 cm in size, or at 308 days of age. We prepared mammary tumors and mammary gland whole mounts for histologic analysis. There were no significant effects when only the malignant or only the benign tumors were considered. After all benign and malignant lesions were pooled, the number of mammary tumors differed among all MNU-treated groups (p = 0.02) with more lesions developing in the MNU-1,000[times] (median = 4.5; p = 0.05) and MNU-TCDD (median = 5.5; p = 0.07) compared to the MNU-0 rats (median = 2). Compared to the MNU-0 group, the percentage of rats that developed palpable tumors (benign plus malignant) was slightly higher (p = 0.06) in the MNU-TCDD group, but not in the MNU-1,000[times] group. The percentage of palpable tumors that were malignant was higher (p = 0.02) in the MNU-100[times] group (15/16, 94%) than in the MNU-0 group (10/18, 56%). The highest dose of the mixture delayed (p = 0.03) the development of tumors, but this was not observed with the MNU-TCDD treatment. These results suggest that neonatal exposure to high doses of organochlorines could favor the development of MNU-induced mammary lesions, but also delays the development of palpable tumors in the rat.
authors.affiliation: Environmental and Occupational Toxicology Division, Bureau of Chemical Hazards, Department of Health, Ottawa, Ontario, Canada, K1A 0L2. Daniel_Desaulniers@hc-sc.gc.ca
authors.forename: D
authors.initials: D
authors.lastname: Desaulniers
authors.forename: K
authors.initials: K
authors.lastname: Leingartner
authors.forename: J
authors.initials: J
authors.lastname: Russo
authors.forename: G
authors.initials: G
authors.lastname: Perkins
authors.forename: B G
authors.initials: BG
authors.lastname: Chittim
authors.forename: M C
authors.initials: MC
authors.lastname: Archer
authors.forename: M
authors.initials: M
authors.lastname: Wade
authors.forename: J
authors.initials: J
authors.lastname: Yang
mesh_terms: D000477:Alkylating Agents / Q000009:adverse effects / Q000494:pharmacology*; D000818:Animals; D001940:Breast / Q000473:pathology; D002471:Cell Transformation, Neoplastic; D003634:DDT / Q000009:adverse effects* / Q000493:pharmacokinetics; D003633:Dichlorodiphenyl Dichloroethylene / Q000009:adverse effects* / Q000493:pharmacokinetics; D004305:Dose-Response Relationship, Drug; D004785:Environmental Pollutants / Q000009:adverse effects* / Q000493:pharmacokinetics; D005260:Female; D007306:Insecticides / Q000009:adverse effects* / Q000493:pharmacokinetics; D008325:Mammary Neoplasms, Experimental / Q000139:chemically induced*; D008770:Methylnitrosourea / Q000009:adverse effects / Q000494:pharmacology*; D011078:Polychlorinated Biphenyls / Q000009:adverse effects* / Q000493:pharmacokinetics; D000072317:Polychlorinated Dibenzodioxins / Q000009:adverse effects* / Q000493:pharmacokinetics; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000477:Alkylating Agents; D004785:Environmental Pollutants; D007306:Insecticides; D000072317:Polychlorinated Dibenzodioxins; D003633:Dichlorodiphenyl Dichloroethylene; D008770:Methylnitrosourea; D003634:DDT; D011078:Polychlorinated Biphenyls