Enzymes and chelating agent in cotton pretreatment.
Desized cotton fabric and cotton seed-coat fragments (impurities) have been treated with commercial cellulase (Celluclast 1.5 L), hemicellulase-pectinase (Viscozyme 120 L) and xylanase (Pulpzyme HC) enzymes. Seed-coat fragments hydrolyzed much faster than the cotton fabric itself. This relative difference in hydrolysis rates makes possible a direct enzymatic removal of seed-coat fragments from desized cotton fabric. Addition of chelating agents such as ethylenediamine-tetra-acetic acid (EDTA) markedly enhanced the directed enzyme action. Pretreatments carried out in acidic solution at pH 5 increased the lightness of seed-coat fragments, contrary to the samples treated in neutral medium at pH 7. Alkaline scouring resulted in darker seed-coat fragments except for the samples pretreated with Pulpzyme HC plus EDTA. This effect is similar to that observed in the biobleaching process in pulp and paper industry.
Csiszár
E
E
Budapest University of Technology and Economics, PO Box 91, H-1521, Budapest, Hungary. emi.oct@chem.bme.hu
Losonczi
A
A
Szakács
G
G
Rusznák
I
I
Bezúr
L
L
Reicher
J
J
D002118:Calcium / Q000737:chemistry; D002480:Cellulase / Q000737:chemistry*; D002614:Chelating Agents / Q000737:chemistry*; D004492:Edetic Acid; D006026:Glycoside Hydrolases / Q000737:chemistry*; D003368:Gossypium; D006868:Hydrolysis; D011096:Polygalacturonase / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry; D043325:Xylan Endo-1,3-beta-Xylosidase; D014995:Xylosidases / Q000737:chemistry*
D002614:Chelating Agents; D004492:Edetic Acid; D006026:Glycoside Hydrolases; D014995:Xylosidases; C023305:hemicellulase; D011096:Polygalacturonase; D043325:Xylan Endo-1,3-beta-Xylosidase; D002480:Cellulase; D002118:Calcium