title: [Detection of hepatitis C virus RNA in tear film of a patient with recurrent peripheral corneal ulcers].
abstract: BACKGROUND
  There are conflicting reports on the role of hepatitis C virus in corneal pathology.
  
  
  METHODS
  A 58-year-old male patient presented with recurrent peripheral corneal ulcers and corneal thinning in the left eye. There was a bilateral vascular pannus formation and a decreased ocular wetting measured by Schirmer testing. The posterior ocular segment was normal. There was no sign of any systemic rheumatic disease. Serological testing detected antibodies against hepatitis C virus. Hepatitis C virus RNA testing using a quantitative polymerase chain reaction method revealed hepatitis C virus RNA in serum (> 3.2 million copies/ml) and in tear samples (18,000 copies/ml) of the patient. In a control group of 7 consecutive patients with hepatitis C virus RNA detection in the serum but without ocular pathology, no hepatitis C virus RNA was detected in tear samples (detection limit: 1000 copies/ml).
  
  
  CONCLUSIONS
  Detection of hepatitis C virus RNA in lacrimal fluid of a patient with recurrent peripheral corneal ulcers may indicate a pathogenic role of hepatitis C virus in corneal pathology. Especially, since our patients with systemic hepatitis C virus infection but without ocular changes did not show hepatitis C virus RNA in their tears. Therefore, patients with recurrent corneal ulcers of unknown origin should be tested for systemic hepatitis C virus infection.
authors.affiliation: Augenklinik mit Poliklinik, Universität Erlangen-Nürnberg, Schwabachanlage 6, 91054 Erlangen. hartmut.wenkel@augen.imed.uni-erlangen.de
authors.forename: H
authors.initials: H
authors.lastname: Wenkel
authors.forename: D
authors.initials: D
authors.lastname: Krist
authors.forename: K
authors.initials: K
authors.lastname: Korn
mesh_terms: D003320:Corneal Ulcer / Q000175:diagnosis* / Q000821:virology; D003937:Diagnosis, Differential; D016174:Hepacivirus / Q000235:genetics / Q000472:pathogenicity; D006526:Hepatitis C / Q000175:diagnosis* / Q000821:virology; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000032:analysis*; D012008:Recurrence; D013666:Tears / Q000821:virology*; D014774:Virulence
chemical_list: D012367:RNA, Viral