title: An unmediated hydrogen peroxide biosensor based on hemoglobin incorporated in a montmorillonite membrane.
abstract: Hemoglobin was incorporated in a montmorillonite membrane. Electrochemical and spectroscopic studies revealed that the electron transfer reactivity and peroxidase activity of the protein were both enhanced. Nevertheless, its structure was still maintained native-like in the membrane. An unmediated hydrogen peroxide biosensor was accordingly prepared. The calibration plot of this H202 sensor was linear in the range of 1.0 x 10(-6)-6.0 x 10(-4) mol L(-1). The relative standard deviation was 3.1% for six successive determinations at a concentration of 1.0 x 10(-4) mol L(-1). The detection limit was 6.0 x 10(-7) mol L(-1). Possible interferences in real sample analyses are discussed.
authors.affiliation: Department of Biochemistry and National Laboratory of Pharmaceutical Biotechnology, Nanjing University, China.
authors.forename: C
authors.initials: C
authors.lastname: Fan
authors.forename: Q
authors.initials: Q
authors.lastname: Gao
authors.forename: D
authors.initials: D
authors.lastname: Zhu
authors.forename: G
authors.initials: G
authors.lastname: Wagner
authors.forename: G
authors.initials: G
authors.lastname: Li
mesh_terms: D000818:Animals; D015374:Biosensing Techniques; D002417:Cattle; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods; D006454:Hemoglobins; D006861:Hydrogen Peroxide / Q000032:analysis*; D013055:Spectrophotometry, Infrared
chemical_list: D006454:Hemoglobins; D006861:Hydrogen Peroxide