title: Revascularisation of fresh compared with demineralised bone grafts in rats.
abstract: Revascularisation of bone grafts is influenced by both the anatomical origin and the pre-implantation processing of the graft. We investigated the revascularisation by entrapment of 141Ce (cerium)-labelled microspheres in large, fresh and demineralised syngeneic grafts of predominantly cancellous (iliac bone) or cortical (tibial diaphysis) bone three weeks after heterotopic implantation in rats. The mean (SD) 141Ce deposition index (counts per minute (cpm) of mg recovered implant/cpm of mg host iliac bone) was higher in fresh iliac bone grafts, 0.98 (0.46) compared to that of demineralised iliac bone, 0.32 (0.20), p < 0.001, and fresh tibial bone grafts, 0.51 (0.27), p = 0.007. We found no significant difference in the mean 141Ce deposition index between fresh tibial bone grafts and demineralised tibial bone grafts, 0.35 (0.42), p = 0.4, or between demineralised tibial grafts and demineralised iliac bone grafts, p = 0.8. The results suggest that whereas fresh cancellous grafts are revascularised more completely than fresh cortical grafts, there is no difference in the revascularisation of demineralised cancellous and cortical grafts. In addition, fresh cancellous bone is revascularised more completely than demineralised cancellous bone, whereas there is no difference between fresh and demineralised cortical bone.
authors.affiliation: Institute for Surgical Research, Rikshospitalet, University of Oslo, Oslo, Norway. eirik.solheim@eunet.no
authors.forename: E
authors.initials: E
authors.lastname: Solheim
authors.forename: E M
authors.initials: EM
authors.lastname: Pinholt
authors.forename: O
authors.initials: O
authors.lastname: Talsnes
authors.forename: T B
authors.initials: TB
authors.lastname: Larsen
authors.forename: O J
authors.initials: OJ
authors.lastname: Kirkeby
mesh_terms: D000818:Animals; D018775:Bone Demineralization Technique; D016025:Bone Transplantation / Q000379:methods / Q000502:physiology*; D001842:Bone and Bones / Q000098:blood supply* / Q000502:physiology; D002565:Cerium Radioisotopes; D007085:Ilium / Q000098:blood supply / Q000502:physiology / Q000637:transplantation; D008297:Male; D008863:Microspheres; D018919:Neovascularization, Physiologic; D051381:Rats; D011917:Rats, Inbred Lew; D013977:Tibia / Q000098:blood supply / Q000502:physiology / Q000637:transplantation; D016042:Transplantation, Heterotopic / Q000502:physiology*
chemical_list: D002565:Cerium Radioisotopes