A plant-specific cyclin-dependent kinase is involved in the control of G2/M progression in plants.
Cyclin-dependent kinases (CDKs) control the key transitions in the eukaryotic cell cycle. All the CDKs known to control G(2)/M progression in yeast and animals are distinguished by the characteristic PSTAIRE motif in their cyclin-binding domain and are closely related. Higher plants contain in addition a number of more divergent non-PSTAIRE CDKs with still obscure functions. We show that a plant-specific type of non-PSTAIRE CDKs is involved in the control of the G(2)/M progression. In synchronized tobacco BY-2 cells, the corresponding protein, accumulated in a cell cycle-regulated fashion, peaking at the G(2)/M transition. The associated histone H1 kinase activity reached a maximum in mitosis and required a yet unidentified subunit to be fully active. Down-regulation of the associated kinase activity in transgenic tobacco plants using a dominant-negative mutation delayed G(2)/M transition. These results provide the first evidence that non-PSTAIRE CDKs are involved in the control of the G(2)/M progression in plants.
Porceddu
A
A
Vakgroep Moleculaire Genetica and Departement Plantengenetica, Vlaams Interuniversitair Instituut voor Biotechnologie (VIB), Universiteit Gent, B-9000 Gent, Belgium.
Stals
H
H
Reichheld
J P
JP
Segers
G
G
De Veylder
L
L
Barroco
R P
RP
Casteels
P
P
Van Montagu
M
M
Inzé
D
D
Mironov
V
V
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D029681:Arabidopsis Proteins; D001667:Binding, Competitive; D002453:Cell Cycle; D002467:Cell Nucleus; D002478:Cells, Cultured; D002850:Chromatography, Gel; D018844:Cyclin-Dependent Kinases / Q000235:genetics* / Q000502:physiology*; D015536:Down-Regulation; D005434:Flow Cytometry; D016195:G2 Phase; D005799:Genes, Dominant; D006657:Histones / Q000378:metabolism; D015151:Immunoblotting; D008938:Mitosis; D008969:Molecular Sequence Data; D009154:Mutation; D030821:Plants, Genetically Modified / Q000378:metabolism; D010947:Plants, Toxic; D010957:Plasmids / Q000378:metabolism; D011233:Precipitin Tests; D011485:Protein Binding; D011494:Protein Kinases / Q000378:metabolism; D017434:Protein Structure, Tertiary; D017386:Sequence Homology, Amino Acid; D013997:Time Factors; D014026:Nicotiana / Q000166:cytology; D019076:Transgenes
D029681:Arabidopsis Proteins; D006657:Histones; D011494:Protein Kinases; C102333:histone H1 kinase; C434472:CDKA1 protein, Arabidopsis; D018844:Cyclin-Dependent Kinases