title: Genetic variation of Citrus tristeza virus isolates from California and Spain: evidence for mixed infections and recombination.
abstract: We examined the population structure and genetic variation of four genomic regions within and between 30 Citrus tristeza virus (CTV) isolates from Spain and California. Our analyses showed that most isolates contained a population of sequence variants, with one being predominant. Four isolates showed two major sequence variants in some genomic regions. The two major variants of three of these isolates showed very low nucleotide identity to each other but were very similar to those of other isolates, suggesting the possibility of mixed infections with two divergent isolates. Incongruencies of phylogenetic relationships in the different genomic regions and statistical analyses suggested that the genomes of some CTV sequence variants originated by recombination events between diverged sequence variants. No correlation was observed between geographic origin and nucleotide distance, and thus from a genetic view, the Spanish and Californian isolates analyzed here could be considered members of the same population.
authors.affiliation: Plant Pathology Department, University of California, Davis, California 95616, USA.
authors.forename: L
authors.initials: L
authors.lastname: Rubio
authors.forename: M A
authors.initials: MA
authors.lastname: Ayllón
authors.forename: P
authors.initials: P
authors.lastname: Kong
authors.forename: A
authors.initials: A
authors.lastname: Fernández
authors.forename: M
authors.initials: M
authors.lastname: Polek
authors.forename: J
authors.initials: J
authors.lastname: Guerri
authors.forename: P
authors.initials: P
authors.lastname: Moreno
authors.forename: B W
authors.initials: BW
authors.lastname: Falk
mesh_terms: D002140:California; D002957:Citrus / Q000821:virology*; D003001:Cloning, Molecular; D017790:Closterovirus / Q000235:genetics* / Q000302:isolation & purification; D018076:DNA, Complementary; D014644:Genetic Variation; D016679:Genome, Viral; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000821:virology*; D016133:Polymerase Chain Reaction / Q000379:methods; D018807:Polymorphism, Single-Stranded Conformational; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA; D013030:Spain
chemical_list: D018076:DNA, Complementary