title: Acquisition of tolerance against oxidative damage in Saccharomyces cerevisiae.
abstract: BACKGROUND
  Living cells constantly sense and adapt to redox shifts by the induction of genes whose products act to maintain the cellular redox environment. In the eukaryote Saccharomyces cerevisiae, while stationary cells possess a degree of constitutive resistance towards oxidants, treatment of exponential phase cultures with sub-lethal stresses can lead to the transient induction of protection against subsequent lethal oxidant conditions. The sensors of oxidative stress and the corresponding transcription factors that activate gene expression under these conditions have not yet been completely identified.
  
  
  RESULTS
  We report the role of SOD1, SOD2 and TPS1 genes (which encode the cytoplasmic Cu/Zn-superoxide dismutase, the mitochondrial Mn-isoform and trehalose-6-phosphate synthase, respectively) in the development of resistance to oxidative stress. In all experimental conditions, the cultures were divided into two parts, one was immediately submitted to severe stress (namely: exposure to H2O2, heat shock or ethanol stress) while the other was initially adapted to 40 degrees C for 60 min. The deficiency in trehalose synthesis did not impair the acquisition of tolerance to H2O2, but this disaccharide played an essential role in tolerance against heat and ethanol stresses. We also verified that the presence of only one Sodp isoform was sufficient to improve cellular resistance to 5 mM H2O2. On the other hand, while the lack of Sod2p caused high cell sensitivity to ethanol and heat shock, the absence of Sod1p seemed to be beneficial to the process of acquisition of tolerance to these adverse conditions. The increase in oxidation-dependent fluorescence of crude extracts of sod1 mutant cells upon incubation at 40 degrees C was approximately 2-fold higher than in sod2 and control strain extracts. Furthermore, in Western blots, we observed that sod mutants showed a different pattern of Hsp104p and Hsp26p expression also different from that in their control strain.
  
  
  CONCLUSIONS
  Trehalose seemed not to be essential in the acquisition of tolerance to H2O2 stress, but its absence was strongly felt under water stress conditions such as heat and alcoholic stresses. On the other hand, Sod1p could be involved in the control of ROS production; these reactive molecules could signal the induction of genes implicated within cell tolerance to heat and ethanol. The effects of this deletion needs further investigation.
authors.affiliation: Depart Bioquímica, Inst Química, UFRJ, 21949-900 Rio de Janeiro, RJ, Brazil. marcosdp@iq.ufrj.br
authors.forename: M D
authors.initials: MD
authors.lastname: Pereira
authors.forename: E C
authors.initials: EC
authors.lastname: Eleutherio
authors.forename: A D
authors.initials: AD
authors.lastname: Panek
mesh_terms: D006360:Heat-Shock Proteins / Q000378:metabolism; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000502:physiology*; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000378:metabolism / Q000502:physiology*; D013482:Superoxide Dismutase / Q000378:metabolism; D014199:Trehalose / Q000494:pharmacology
chemical_list: D006360:Heat-Shock Proteins; D014199:Trehalose; D013482:Superoxide Dismutase