Effects of the cannabimimetic fatty acid derivatives 2-arachidonoylglycerol, anandamide, palmitoylethanolamide and methanandamide upon IgE-dependent antigen-induced beta-hexosaminidase, serotonin and TNF alpha release from rat RBL-2H3 basophilic leukaemic cells.
There are conflicting reports in the literature as to whether palmitoylethanolamide affects the function of mast cell-related cell lines in vitro, in contrast to the well-documented effects of this compound upon mast cell function in vivo. In the present study, we have reinvestigated the effects of palmitoylethanolamide upon antigen-induced release of [3H]serotonin and beta-hexosaminidase from rat basophilic leukemia RBL-2H3 cells and compared these effects with those of 2-arachidonoylglycerol, anandamide and R1-methanandamide. RBL-2H3 cells were sensitized with a monoclonal anti-DNP IgE, after which they were stimulated with antigen (DNP-HSA). Palmitoylethanolamide produced a small, but significant reduction in antigen-stimulated [3H]serotonin release at high concentrations, whereas anandamide was without effect. In contrast, 2-arachidonoylglycerol and methanandamide increased the antigen-stimulated release of both [3H]serotonin and beta-hexosaminidase. It is concluded that in RBL-2H3 cells, these cannabimimetic fatty acid derivatives do not have potent stabilizing effects upon antigen-induced degranulation.
Granberg
M
M
Department of Pharmacology and Clinical Neuroscience, Umeå University, Sweden.
Fowler
C J
CJ
Jacobsson
S O
SO
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000577:Amides; D000818:Animals; D001095:Arachidonic Acids / Q000494:pharmacology*; D063388:Endocannabinoids; D004790:Enzyme Induction; D004983:Ethanolamines; D005989:Glycerides / Q000494:pharmacology*; D007073:Immunoglobulin E / Q000378:metabolism; D018836:Inflammation Mediators / Q000378:metabolism; D007938:Leukemia; D008024:Ligands; D008407:Mast Cells / Q000276:immunology / Q000378:metabolism; D010169:Palmitic Acids / Q000494:pharmacology; D053284:Polyunsaturated Alkamides; D051381:Rats; D012701:Serotonin / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*; D001619:beta-N-Acetylhexosaminidases / Q000096:biosynthesis / Q000378:metabolism*
D000276:Adjuvants, Immunologic; D000577:Amides; D001095:Arachidonic Acids; D063388:Endocannabinoids; D004983:Ethanolamines; D005989:Glycerides; D018836:Inflammation Mediators; D008024:Ligands; D010169:Palmitic Acids; D053284:Polyunsaturated Alkamides; D014409:Tumor Necrosis Factor-alpha; C088155:methanandamide; D012701:Serotonin; D007073:Immunoglobulin E; C005958:palmidrol; C094503:glyceryl 2-arachidonate; D001619:beta-N-Acetylhexosaminidases; C078814:anandamide