[Effect of Sindbis XJ-160 virus structural proteins on BHK-21 cell].
OBJECTIVE
To study the effect of Sindbis XJ-160 virus structural proteins on BHK-21 cell.


METHODS
We constructed the expressing vector pcDNA3.1 ABC that contains the XJ-160 virus structural protein gene and then transfected it to BHK-21 cell.


RESULTS
The expressed products can be detected by RNA dot blot hybridization or immUnofluorescent assay. The cytopathic effect was observed in BHK-21 cells transfected with pcDNA3.1 ABC. There was also condensed chromatin found accumulated. BHK-21 cell death and marked cell cycle changes were observed.


CONCLUSIONS
These results indicate that cell apoptosis might be one of the mechanisms inducing cell death by XJ - 160 virus and its structural protein gene probably plays a role in this programmed cell death.
Li
L
L
State Key Laboratory for Molecular Virology and Genetic Engineering, Institute of Virology, Chinese Academy of Preventive Medicine, Beijing 100052, China.
Liang
G
G
Zhao
B
B
D017209:Apoptosis / Q000235:genetics*; D002213:Capsid; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral / Q000235:genetics*; D015870:Gene Expression; D012845:Sindbis Virus / Q000737:chemistry / Q000235:genetics*; D014162:Transfection; D015678:Viral Structural Proteins / Q000235:genetics*
D015678:Viral Structural Proteins