[A research on rat osteoblasts culture in vitro].
Osteoblasts were isolated from Sprague Dawley rats of 1-3 days on the frontal parietal bones and were confirmed by testing for multiple biological features. The results showed that rat osteoblasts in vitro had the same morphological feature, ALP activity, the function of synthetic BMP as in vivo and it had the ability of calcification in vitro. The results proved that these rat osteoblasts in vitro had the same function and features as in vivo and it could be used to study cytocompatibility on replacement materials of human body's hard tissues and biomedical to make experiments related to bone tissues.
Tang
Z
Z
College of Stomatology, West China University of Medical Sciences.
Chen
Z
Z
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D019485:Bone Morphogenetic Proteins / Q000378:metabolism; D002113:Calcification, Physiologic; D002478:Cells, Cultured; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism; D010294:Parietal Bone / Q000166:cytology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D019485:Bone Morphogenetic Proteins; D000469:Alkaline Phosphatase