[IGF-I mRNA expression level of cell in the interfaces of bone and four kinds of substitutive materials for bone tissues by the technique in situ hybridization].
Four kinds of substitutive materials for hard tissues were inserted into tibias of rat, effect of substitutive materials on IGF-I mRNA expression level of cell in the interfaces of bone and four kinds of substitutive materials were studied by the technique in situ hybridization. It showed that the biomaterials could affect the level of IGF-I mRNA expression, the enhanced expression level was associated with cell proliferation, formation of matrix and calcification, formation of new bone. Measurement of IGF-I mRNA could attribute to judge the functional state of cell, and to predict the final character of the interface.
Ou
G
G
College of Stomatology, West China University of Medical Sciences, China.
Chen
Z
Z
D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D018786:Bone Substitutes / Q000494:pharmacology*; D003722:Dental Alloys / Q000494:pharmacology; D003776:Dental Porcelain / Q000494:pharmacology; D006882:Hydroxyapatites / Q000494:pharmacology; D017403:In Situ Hybridization; D007334:Insulin-Like Growth Factor I / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013977:Tibia / Q000293:injuries / Q000378:metabolism* / Q000601:surgery; D014025:Titanium / Q000494:pharmacology
D001672:Biocompatible Materials; D018786:Bone Substitutes; D003722:Dental Alloys; D006882:Hydroxyapatites; D012333:RNA, Messenger; D003776:Dental Porcelain; D007334:Insulin-Like Growth Factor I; D014025:Titanium