Ecdysone-controlled mRNA stability in Drosophila salivary glands: deadenylation-independent degradation of larval glue protein gene message during the larval/prepupal transition.
20-Hydroxyecdysone induces poly(A) shortening and the subsequent degradation of transcripts encoding the larval glue protein LGP-1 in Drosophila virilis late third larval instar salivary glands. Degradation concurs with the transient increase of ribonucleolytic activities in the gland cells. In vitro nuclease assays using crude cytoplasmic extracts of ecdysone-treated salivary glands demonstrate degradation to be deadenylation-independent and that the induced ribonucleolytic activities initiate the degradation of the Lgp-1 transcripts in putative single-stranded loop regions. The independence of degradation from deadenylation is also found in vivo in transformed D. melanogaster carrying a modified Lgp-1 gene.
Kress
H
H
Institut für Biologie-Genetik, Freie Universität Berlin, Arnimallee 7, D-14 195 Berlin, Germany. kress@zedat.fu-berlin.de
Kunze
P
P
Swida
U
U
Heiser
V
V
Maletz
S
S
D000818:Animals; D030801:Animals, Genetically Modified; D046508:Culture Techniques; D004330:Drosophila / Q000235:genetics*; D004440:Ecdysone / Q000494:pharmacology*; D004790:Enzyme Induction / Q000187:drug effects; D005967:Glue Proteins, Drosophila / Q000235:genetics; D007814:Larva / Q000235:genetics; D008675:Metamorphosis, Biological; D008958:Models, Molecular; D011061:Poly A / Q000378:metabolism; D020871:RNA Stability / Q000187:drug effects*; D012333:RNA, Messenger / Q000378:metabolism; D012260:Ribonucleases / Q000187:drug effects / Q000378:metabolism; D012469:Salivary Glands / Q000378:metabolism*
D005967:Glue Proteins, Drosophila; D012333:RNA, Messenger; D011061:Poly A; D004440:Ecdysone; D012260:Ribonucleases