Adhesion development and the expression of endothelial nitric oxide synthase.
OBJECTIVE
This study was conducted to determine whether nitric oxide (NO), a potent vasodilator and inhibitor of thrombus formation, is involved in the formation and maintenance of adhesions.


METHODS
Skin, subcutaneous tissues, peritoneum and adhesions were collected from surgical patients and total RNA was isolated. Quantitative reverse transcription polymerase chain reaction (QRT-PCR) was performed to quantitate endothelial nitric oxide synthase (eNOS) and beta-actin mRNA levels.


RESULTS
eNOS mRNA levels for skin, subcutaneous tissue, peritoneum and adhesions were < or = 3.12 x 10(-4), < or = 3.12 x 10(-4), 6.24 x 10(-4) and 2.5 x 10(-3) attomoles/microl, respectively. Beta-actin mRNA levels for all tissues were between 1.25 x 10(-1) and 6.25 x 10(-2) attomoles/microl.


CONCLUSIONS
eNOS mRNA can be identified in tissue adhesions, and may therefore play a role in adhesion formation and maintenance.
Svinarich
D M
DM
Department of Obstetrics and Gynecology, Wayne State University School of Medicine, Detroit, MI 48201, USA. d.svinarich@wayne.edu
Zaher
F M
FM
Holmdahl
L
L
Chegini
N
N
Gonik
B
B
Diamond
M P
MP
D000199:Actins / Q000032:analysis; D004727:Endothelium / Q000503:physiopathology; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D019001:Nitric Oxide Synthase / Q000502:physiology*; D010532:Peritoneal Diseases / Q000235:genetics / Q000503:physiopathology*; D010537:Peritoneum / Q000503:physiopathology; D010865:Pilot Projects; D012333:RNA, Messenger / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012867:Skin / Q000503:physiopathology; D000267:Tissue Adhesions / Q000235:genetics / Q000503:physiopathology*
D000199:Actins; D012333:RNA, Messenger; D019001:Nitric Oxide Synthase