Antiplatelet agent cilostazol potentiates adipocyte differentiation of 3T3-L1 cells.
Cilostazol is an antiplatelet drug, which has beneficial effects in treatment of intermittent claudication and decreases serum triacyiglycerol level in these patients. In this study, we examined adipogenic potency of cilostazol using 3T3-L1 preadipocyte cell line because cilostazol is one of the tissue specific phosphodiesterase (PDE) inhibitors. Addition of cilostazol into the differentiation medium including insulin and dexamethasone, induced the adipocyte differentiation without isobutyl methylxanthine (IBMX). Compared with the cells incubated with vehicle, the cells treated with cilostazol contain much more lipid droplets in the cells 6 days after induction of differentiation. Adipocyte specific gene like stearoyl-CoA desaturase was strongly induced after addition of cilostazol. C/EBPbeta, which is induced by IBMX was also induced by cilostazol. These findings suggest a possibility that adipogenic effect of cilostazol is one of the mechanisms, by which this agent decreases blood triacylglycerol level in the intermittent claudication patients.
Hirose
T
T
Department of Internal Medicine, Nishinomiya Municipal Central Hospital, 8-24 Hayashidacho Nishinomiya, Hyogo 663-8014, Japan. tak1_h@d5.dion.ne.jp
Kurebayashi
S
S
Kasayama
S
S
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D016475:3T3 Cells; D017667:Adipocytes / Q000187:drug effects* / Q000502:physiology; D000818:Animals; D022782:CCAAT-Enhancer-Binding Protein-beta / Q000235:genetics; D002454:Cell Differentiation / Q000187:drug effects; D000077407:Cilostazol; D003907:Dexamethasone / Q000494:pharmacology; D005786:Gene Expression Regulation; D007328:Insulin / Q000494:pharmacology; D051379:Mice; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology*; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D013230:Stearoyl-CoA Desaturase / Q000235:genetics; D013777:Tetrazoles / Q000494:pharmacology*
D022782:CCAAT-Enhancer-Binding Protein-beta; D007328:Insulin; D010726:Phosphodiesterase Inhibitors; D010975:Platelet Aggregation Inhibitors; D013777:Tetrazoles; D003907:Dexamethasone; D013230:Stearoyl-CoA Desaturase; D000077407:Cilostazol; D015056:1-Methyl-3-isobutylxanthine