[In vitro activity of ascorbic acid].
The aim of this study was to determine the in vitro activity of ascorbic acid (vitamin C) against 43 Helicobacter pylori clinical isolates obtained from gastric biopsy taken by endoscopy. The MIC was determined by an agar dilution method. The ascorbic acid was included as two-fold dilutions in the agar to obtain a final concentration from 4000 mg/l to 62 mg/l. Brain-heart infusion agar supplemented with yeast extract and 10% fetal calf serum, 10(6) CFU/spot inoculum and microaerobic incubation for 2 to 5 days were used. MIC(50) 500 mg/l, MIC(90) 1000 mg/l and the range from 62 to 2000 mg/l. No differences in susceptible or resistant isolates were observed. Ascorbic acid showed good in vitro activity against the H. pylori clinical isolates tested in this study, which could explain some of the beneficial properties of vitamin C.
López-Brea
M
M
Servicio de Microbiología, Hospital Universitario de la Princesa, C/Diego de León n masculine 62, 28006 Madrid.
Domingo
D
D
Alarcón
T
T
D001205:Ascorbic Acid / Q000494:pharmacology*; D016480:Helicobacter pylori / Q000187:drug effects*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008826:Microbial Sensitivity Tests
D001205:Ascorbic Acid