[Effects of phenytoin on structural aberration of human sperm chromosomes in vitro].
OBJECTIVE
To detect the mutagenic effects of phenytoin on human sperm chromosomes.


METHODS
The mutagenic effects of phenytoin at 10, 20 and 40 microgram/ml were tested by an in vitro testing system of human sperm chromosomes, with leomycin A5 as a positive control and mutagen-free solution as the blank control.


RESULTS
The frequencies of sperm with structural chromosomal aberrations and breakages of sperm chromosomes in the groups of 10 microgram/ml, 20 microgram/ml and 40 ug/ml of phenytoin were all higher than those in the blank control, but only in the group of 40 ug/ml, the elevated frequencies were statistically significant (P<0.005, P<0.05).


CONCLUSIONS
Phenytoin has probably mutagenic potential effect on human sperm cells.
Meng
H
H
Department of Psychiatry, the First Affiliated Hospital, Chongqing University of Medical Sciences, Chongqing 400016 P.R.China. MHQ99666@sina.com
Zhang
F
F
Gao
X
X
Wang
X
X
Li
D
D
Cui
X
X
Wang
Z
Z
D000328:Adult; D000927:Anticonvulsants / Q000494:pharmacology; D002869:Chromosome Aberrations / Q000139:chemically induced*; D002877:Chromosomes, Human / Q000187:drug effects*; D006801:Humans; D007621:Karyotyping; D008297:Male; D010672:Phenytoin / Q000494:pharmacology*; D012661:Semen / Q000187:drug effects* / Q000502:physiology; D013081:Sperm Motility; D013094:Spermatozoa / Q000187:drug effects*
D000927:Anticonvulsants; D010672:Phenytoin