Biotransformation of selamectin with Streptomyces lydicus SX-1298 using a novel static agar fermentation system with Reemay mesh.
A novel approach to biotransformation is described using a solid medium matrix and Reemay mesh that gives efficient biotransformation of compounds with minimal matrices in the ensuing gum solids. Using this approach with a newly isolated biotransforming organism, Streptomyces lydicus SX1298, a series of hydroxylations and an O-demethylation is described for selamectin the first endectocide for cats and dogs.
Pacey
M S
MS
Veterinary Medicine Discovery, Pfizer Global Research and Development, Sandwich, Kent, UK. mike_pacey@sandwich.pfizer.com
Barnes
M M
MM
Monday
R A
RA
Ritzau
M
M
Wellington
E M
EM
D000977:Antiparasitic Agents / Q000737:chemistry / Q000378:metabolism / Q000493:pharmacokinetics*; D001711:Biotransformation; D002853:Chromatography, Liquid; D005285:Fermentation; D007559:Ivermectin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism / Q000493:pharmacokinetics*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D013302:Streptomyces / Q000378:metabolism*
D000977:Antiparasitic Agents; D007559:Ivermectin; C414354:selamectin