[Study on the molecular mechanisms involved in the increased collagen synthesis by platelet-derived wound healing factors during wound healing in alloxan-induced diabetic rat].
OBJECTIVE
To explore the molecular mechanisms involved in the increased collagen synthesis by platelet-derived wound healing factors (PDWHF) during wound healing in alloxan-induced diabetic rats.


METHODS
Thirty-three male SD rats were divided into two groups, the normal (n = 9) (group A) and the diabetic group (n = 24). Two pieces of full-thickness skin with diameter of 1.8 cm were removed from the dorsal site of diabetic rats. PDWHF (100 micrograms/wound) was topically applied to one side of the diabetic wounds (group B) on the operation day and then once a day in the next successive 6 days. Meanwhile, bovine serum albumin (100 micrograms/wound) was applied to the other side of diabetic wound as control group (group C) in the same way. Levels of transforming growth factor-beta 1 (TGF-beta 1) and procollagen I mRNA in wound tissue were inspected by dot blotting.


RESULTS
TGF-beta 1 mRNA levels in group B were 4 folds and 5.6 folds compared with those in group C after 5 and 7 days (P < 0.01), however, still significantly lower than those of group A (P < 0.05). There was no significance difference among three groups on the 10th day after wounding. The levels for procollagen I mRNA in group B amounted to 2.1, 1.8 and 2.3 folds of those in group C after 5, 7, and 10 days (P < 0.01), respectively. Compared with those in the group A, procollagen I mRNA levels in the group B were significantly lower after 5 and 7 days (P < 0.05), and no significant difference was observed between group B and A after 10 days.


CONCLUSIONS
One important way for PDWHF to enhance the collagen synthesis in diabetic wound healing is to increase the gene expression of endogenous TGF-beta 1.
Zhu
X D
XD
Research Institute of Surgery, Third Military Medical University, Chongqing, P. R. China 400042. dongdong@mindless.com
Zhang
Y
Y
Hu
C X
CX
D000818:Animals; D045424:Complex Mixtures; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D006133:Growth Substances / Q000494:pharmacology*; D066298:In Vitro Techniques; D008297:Male; D011347:Procollagen / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D016212:Transforming Growth Factor beta / Q000096:biosynthesis* / Q000235:genetics; D014945:Wound Healing
C078881:CT-102 activated platelet supernatant; D045424:Complex Mixtures; D006133:Growth Substances; D011347:Procollagen; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta