title: Platelet adhesion to heparin coated oxygenator fibers under in vitro static conditions: impact of temperature.
abstract: Heparin coating of cardiopulmonary bypass (CPB) circuitry may attenuate the platelet consumption associated with CPB. We investigated the effect of temperature on the interaction between platelet and heparin coated surfaces under in vitro static conditions. Heparin coated and non coated oxygenator fibers were incubated with heparinized whole blood at 37 degrees C and 22 degrees C. The incubation time was set at 30, 60, 180, and 300 minutes. The number of platelets adhering to each fiber was assessed with enzyme immunoassay using monoclonal antibody against platelet receptor protein CD 61(GPIIbIIIa). As an index of platelet activation, plasma soluble(s) P-selectin levels were measured by enzyme-linked immunosorbent assay. Under normothermia, the number of adherent platelets on the non coated surface increased significantly after 300 min of incubation. Platelet adhesion was reduced significantly by heparin coating of the surface and was kept constant after 300 min. Under hypothermia, heparin coating was also associated with significant reduction of platelet adhesion. The levels of sP-selectin did not correlate with the extent of platelet adhesion. Our results suggest that heparin coating is effective in decreasing platelet adhesion to the synthetic surface tested regardless of the temperature under static conditions. Inhibition of platelet activation on the heparin coated surface may be masked by standard dose heparinization.
authors.affiliation: Department of Anesthesia, Teikyo University Ichihara Hospital, Chiba, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Niimi
authors.forename: Y
authors.initials: Y
authors.lastname: Ishiguro
authors.forename: Y
authors.initials: Y
authors.lastname: Nakata
authors.forename: T
authors.initials: T
authors.lastname: Goto
authors.forename: S
authors.initials: S
authors.lastname: Morita
authors.forename: S
authors.initials: S
authors.lastname: Yamane
mesh_terms: D000925:Anticoagulants / Q000494:pharmacology*; D015703:Antigens, CD / Q000032:analysis; D001777:Blood Coagulation; D001792:Blood Platelets / Q000737:chemistry / Q000166:cytology / Q000502:physiology; D002315:Cardiopulmonary Bypass; D020099:Coated Materials, Biocompatible; D006493:Heparin / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D039661:Integrin beta3; D010973:Platelet Adhesiveness / Q000187:drug effects / Q000502:physiology*; D010976:Platelet Count; D010980:Platelet Membrane Glycoproteins / Q000032:analysis; D013696:Temperature
chemical_list: D000925:Anticoagulants; D015703:Antigens, CD; D020099:Coated Materials, Biocompatible; D039661:Integrin beta3; D010980:Platelet Membrane Glycoproteins; D006493:Heparin