Effects of Matrine on proliferation and differentiation in K-562 cells.
We investigated the effects of Matrine on proliferation by trypan blue exclusion and differentiation by benzidine staining positive cells in K-562 cells, assayed the telomerase activity using PCR-ELISA assay, analyzed cell cycle by fluorescence-activated cell sorter analysis of the DNA content, and also determined the gene expression level of c-myc, N-ras and p53 by northern blot and dot blot analysis. The results showed that with the addition of 0.1 mg/ml Matrine, cell growth was inhibited significantly by 4 days, benizidine-positive cells rose from 1% to 2% in control cells to 15% in treated cells on day 5; treatment of K-562 cells with 0.1 mg/ml Matrine for 5 days resulted in a marked inhibition in telomerase activity, in a manner that correlated with the extent of differentiation; after exposure to Matrine for 72 h, 64.6% cells were arrested in the G1-phase of the cell cycle, the fraction of cells in S-phase had decreased from 56.9% in control cells to 24.4% in differentiated cells, and the levels of N-ras and p53 mRNA were remarkably increased for 24 and 48 h, respectively, c-myc mRNA expression level declined for 24 h and was inhibited significantly for 48 h. Our study confirmed that Matrine plays a significant effect on the inhibition of proliferation cells and inducing differentiation in K-562 cells.
Zhang
L P
LP
Chongqing University of Medical Science, 400046, Chongqing, People's Republic of China.
Jiang
J K
JK
Tam
J W
JW
Zhang
Y
Y
Liu
X S
XS
Xu
X R
XR
Liu
B Z
BZ
He
Y J
YJ
D000470:Alkaloids / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D015870:Gene Expression / Q000187:drug effects; D011905:Genes, ras / Q000187:drug effects; D006801:Humans; D020014:K562 Cells; D009857:Oncogenes / Q000187:drug effects*; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics / Q000378:metabolism; D011807:Quinolizines; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism; D019098:Telomerase / Q000037:antagonists & inhibitors / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism; D000093842:Matrines
D000470:Alkaloids; D000972:Antineoplastic Agents, Phytogenic; D016271:Proto-Oncogene Proteins c-myc; D011807:Quinolizines; D012333:RNA, Messenger; D016159:Tumor Suppressor Protein p53; D019098:Telomerase; D000093842:Matrines