Using positron emission tomography with [(18)F]FDG to predict tumor behavior in experimental colorectal cancer.
This study investigates the relationship between FDG uptake as determined by positron emission tomography (PET) imaging and rates of tumor growth, cellular GLUT1 transporter density, and the activities of hexokinase and glucose-6-phosphatase in a solid tumor implant model. Five different human colorectal xenografts of different growth properties were implanted in athymic rats and evaluated by dynamic (18)F-FDG-PET. The phosphorylating and dephosphorylating activities of the key glycolytic enzymes, hexokinase and glucose-6-phosphatase, were measured in these tumor types by spectrophotometric assays and the expression of GLUT1 glucose transporter protein was determined by immunohistochemistry. Correlations among FDG accumulation, hexokinase activity, and tumor doubling time are reported in these colon xenografts. The results indicate that the activity of tumor hexokinase may be a marker of tumor growth rate that can be determined by (18)F-FDG-PET imaging. PET scanning may not only be a useful tool for staging patients for extent of disease, but may provide important prognostic information concerning the proliferative rates of malignancies.
Burt
B M
BM
Department of Surgery, Memorial Sloan-Kettering Cancer Center, New York, NY 10021, USA.
Humm
J L
JL
Kooby
D A
DA
Squire
O D
OD
Mastorides
S
S
Larson
S M
SM
Fong
Y
Y
D000818:Animals; D002455:Cell Division / Q000502:physiology; D015179:Colorectal Neoplasms / Q000378:metabolism / Q000473:pathology*; D019788:Fluorodeoxyglucose F18; D006801:Humans; D007150:Immunohistochemistry; D007279:Injections, Subcutaneous; D008297:Male; D009004:Monosaccharide Transport Proteins / Q000378:metabolism; D011237:Predictive Value of Tests; D051381:Rats; D011923:Rats, Nude; D013053:Spectrophotometry; D014055:Tomography, Emission-Computed; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured
D009004:Monosaccharide Transport Proteins; D019788:Fluorodeoxyglucose F18