The effects of exogenous interleukin-12 on the induction of immune response to Porphyromonas gingivalis in vivo in mice.
The effects of treatment with exogenous interleukin-12 (IL-12) on the induction of immune response to Porphyromonas gingivalis, a black pigmented periodontopathic oral bacterium in mice, were determined in the present study. An increased footpad swelling representing a delayed type hypersensitivity (DTH) response to P. gingivalis in IL-12-treated mice could be observed, although increasing doses of IL-12 did not produce cumulative effects on this cellular Immune response. Multiple injections with IL-12 also resulted in elevated serum IFN-gamma levels. Treatment with this cytokine the day before, on and after immunization with heat-killed P. gingivalis augmented the levels of serum antigen-specific IgG2a and IgG3 antibodies, but had obviously little or no effects on those of serum antigen-specific IgG1 and IgG2b antibodies. The results of this study suggest that treatment with exogenous IL-12 In P. gingivalis-immunized mice may enhance DTH response and Th1 cell-associated antibody production.
Rochim
A
A
Department of Oral Surgery, Oral Medicine and Oral Biology, Faculty of Dentistry, Gadjah Mada University, Yogyakarta, Indonesia.
Herminajeng
E
E
Sosroseno
W
W
D000276:Adjuvants, Immunologic / Q000627:therapeutic use*; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology; D000918:Antibody Specificity; D016866:Bacteroidaceae Infections / Q000188:drug therapy*; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D000949:Histocompatibility Antigens Class II / Q000187:drug effects / Q000276:immunology; D006968:Hypersensitivity, Delayed / Q000188:drug therapy; D007371:Interferon-gamma / Q000097:blood; D018664:Interleukin-12 / Q000276:immunology* / Q000627:therapeutic use*; D051379:Mice; D008807:Mice, Inbred BALB C; D023421:Models, Animal; D016966:Porphyromonas gingivalis / Q000187:drug effects* / Q000254:growth & development / Q000276:immunology*; D014775:Virus Activation / Q000187:drug effects* / Q000276:immunology*
D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; D000949:Histocompatibility Antigens Class II; D018664:Interleukin-12; D007371:Interferon-gamma