Melanoma induction in a hairless mouse with short-term application of dimethylbenz[a]anthracene.
Melanomas have been induced in hamsters and guinea pigs with short-term, low dose applications of dimethylbenz [a]anthracene (DMBA) alone. In mice, however, melanoma induction has required either croton oil or ultraviolet radiation promotion in addition to DMBA. In this study, we report the development of a malignant melanoma, with metastases, in a hairless mouse after six applications of 0.25% DMBA alone. At sacrifice, a large primary tumour with characteristics of intralesional transformation was present, along with numerous pigmented macules and papules. Metastases were present in lymph nodes and lungs. There was a marked similarity between this melanoma and its precursor lesions and those seen in an earlier, Weiser-Maple guinea pig model, which, in turn, resembled human melanoma.
Kligman
L H
LH
Department of Dermatology, University of Pennsylvania School of Medicine, 202 Clinical Research Building, 415 Curie Boulevard, Philadelphia, Pennsylvania 19104, USA. LoriFBCR@aol.com
Elenitsas
R
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D015127:9,10-Dimethyl-1,2-benzanthracene / Q000008:administration & dosage* / Q000633:toxicity*; D000818:Animals; D002273:Carcinogens / Q000008:administration & dosage* / Q000633:toxicity*; D005260:Female; D008175:Lung Neoplasms / Q000473:pathology / Q000556:secondary; D008207:Lymphatic Metastasis / Q000473:pathology; D008546:Melanoma, Experimental / Q000139:chemically induced* / Q000473:pathology; D051379:Mice; D008812:Mice, Hairless; D012878:Skin Neoplasms / Q000139:chemically induced* / Q000473:pathology; D013997:Time Factors
D002273:Carcinogens; D015127:9,10-Dimethyl-1,2-benzanthracene