Mismatch repair detection (MRD): high-throughput scanning for DNA variations.
Although there are several methods for genotyping previously identified single nucleotide polymorphisms (SNPs), there is a paucity of approaches for high-throughput scanning for unknown variations. Mismatch repair detection (MRD) utilizes a bacterial mismatch repair system in vivo to detect sequence variants in human DNA samples. We describe modifications in MRD that allow a high degree of parallel processing, and use this modified version to accurately scan for variations in 35 different human DNA fragments simultaneously. MRD's potential for high-throughput scanning can be used to identify new SNPs and to comprehensively compare sequences between patients and controls for identifying disease susceptibility alleles.
Faham
M
M
Neurogenetics Laboratory, Department of Psychiatry, University of California San Francisco, San Francisco, CA 94143, USA.
Baharloo
S
S
Tomitaka
S
S
DeYoung
J
J
Freimer
N B
NB
D020137:Base Pair Mismatch; D004247:DNA; D004260:DNA Repair; D005819:Genetic Markers; D014644:Genetic Variation; D005822:Genetic Vectors; D006801:Humans; D019426:Integrases / Q000378:metabolism; D007763:Lac Operon; D012015:Reference Standards; D012680:Sensitivity and Specificity; D014764:Viral Proteins / Q000378:metabolism
D005819:Genetic Markers; D014764:Viral Proteins; D004247:DNA; C045073:Cre recombinase; D019426:Integrases