Differential expression of Gh1 and Gh 2 genes by competitive RT-PCR in rainbow trout pituitary.
The expressions of the GH1 and GH2 genes were examined by competitive RT-PCR and whole-mount in situ hybridization of pituitary of starved rainbow trout (Oncorhynchus mykiss). lambda RNA having GH primers at both 5' and 3' sites of the gene was used for the competitive RT-PCR, and thermostable reverse transcriptase produced a reasonable band when authentic RNA was examined. The amount of GH1 gene expression was significantly (P < 0.05) greater than that of GH2. Although almost the same amount of GH1 gene expression was obtained during the day, it was significantly (P < 0.05) decreased at midnight. However, there was no significant change in GH2 gene expression in the daily cycle. There were also some differences in gene expression in the pituitary: GH2 gene was more widely expressed than GH1. However, the signal intensity of the GH1 gene was greater than that of GH2.
Mori
T
T
Laboratory of Physiology, Hokkaido University, Hakodate 041-8611, Japan. tmori@pop.fish.hokudai.ac.ip
Deguchi
F
F
Ueno
K
K
D000818:Animals; D015870:Gene Expression; D013006:Growth Hormone / Q000235:genetics*; D017403:In Situ Hybridization; D017686:Oncorhynchus mykiss / Q000235:genetics* / Q000378:metabolism; D010902:Pituitary Gland / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction
D012333:RNA, Messenger; D013006:Growth Hormone