Application of mixed partition-adsorption systems in high-performance liquid chromatography of purines and pyrimidines.
Separation of the test mixtures of some purine and pyrimidine derivatives on silicas (types A and B) in adsorption normal-phase (A-NP) and mixed partition-adsorption normal-phase (MPA-NP) mode has been studied. When the A-NP mode is used the peak shapes are unsatisfactory (especially on type A silica). At the same time MPA-NP systems show a good peak symmetry on all silica types. The findings have demonstrated that the MPA-NP mode offers a specific selectivity. This allows MPA-NP systems to supersede reversed-phase systems in some application areas.
Kazoka
H
H
Latvian Institute of Organic Synthesis, Riga. helena@osi.lv
D000327:Adsorption; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D011687:Purines / Q000737:chemistry / Q000302:isolation & purification*; D011743:Pyrimidines / Q000737:chemistry / Q000302:isolation & purification*
D011687:Purines; D011743:Pyrimidines