title: The role of the natural polyamine putrescine in defense against oxidative stress in Escherichia coli.
abstract: Putrescine up-regulated, in a concentration-dependent manner, the expression levels of the oxyR and katG genes of Escherichia coli cells exposed to hydrogen peroxide. Its stimulatory effect was more pronounced under conditions of strong oxidative stress. 1,4-Diamino-2-butanone, a specific inhibitor of putrescine synthesis, also inhibited oxyR expression under oxidative stress. When added to inhibited cells, putrescine relieved this inhibitory effect. Addition of putrescine to E. coli cultures exposed to oxidative stress led to increased cell survival.
authors.affiliation: Russian Academy of Sciences, Institute of Ecology and Genetics of Microorganisms, Golev St. 13, Perm, 614081, Russia. agtkachenko@ecology.psu.ru
authors.forename: A
authors.initials: A
authors.lastname: Tkachenko
authors.forename: L
authors.initials: L
authors.lastname: Nesterova
authors.forename: M
authors.initials: M
authors.lastname: Pshenichnov
mesh_terms: D002374:Catalase / Q000235:genetics; D002455:Cell Division / Q000187:drug effects; D004268:DNA-Binding Proteins; D004926:Escherichia coli / Q000166:cytology / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects*; D005798:Genes, Bacterial / Q000235:genetics; D006861:Hydrogen Peroxide / Q000494:pharmacology; D016877:Oxidants / Q000494:pharmacology; D018384:Oxidative Stress / Q000187:drug effects*; D011700:Putrescine / Q000494:pharmacology*; D012097:Repressor Proteins / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D016877:Oxidants; D012097:Repressor Proteins; D014157:Transcription Factors; C499299:oxyR protein, E coli; D006861:Hydrogen Peroxide; D002374:Catalase; C051253:katG protein, E coli; D011700:Putrescine