title: Anticholinesterase treatment of chicken retinal cells increases acetylcholinesterase protein independently of protein kinase C.
abstract: It has been reported that anticholinesterase exposure, e.g. by environmental toxins or nerve gases, can increase acetylcholinesterase (AChE) protein, possibly as an autoregulatory stress response. We earlier have transfected retinal cells of the chick embryo with a pSVK3-AChE(rab)-cDNA vector to heterologously express rabbit AChE, which concomitantly also increased AChE protein from chick. To analyse further the cell-internal pathways of these different paradigms (anticholinesterase treatment vs. AChE transfection) which both lead to an AChE increase, we here show that AChE overexpression by transfection leads to an increase in protein kinase C (PKC). Most remarkably, when cells independently of, or in addition to their transfection are treated with 10 microM of the AChE inhibitor BW284c51, AChE protein levels are much more dramatically increased up to 20-fold. This treatment, however, does not affect PKC. These data show that (i) retinal cells respond to anticholinesterase insult by a massive increase of AChE protein; (ii) the response to BW284c51 is not PKC-mediated; and (iii) both strategies of AChE increase follow different cell-internal pathways, their effects being additive. The ecological and biomedical implications of these findings are briefly discussed.
authors.affiliation: Department of Developmental Biology & Neurogenetics, Faculty of Biology, Darmstadt University of Technology, Schnittspahnstrasse 3, D-64287, Darmstadt, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Keller
authors.forename: A
authors.initials: A
authors.lastname: Robitzki
authors.forename: P G
authors.initials: PG
authors.lastname: Layer
mesh_terms: D000110:Acetylcholinesterase / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D000818:Animals; D002093:Benzenaminium, 4,4'-(3-oxo-1,5-pentanediyl)bis(N,N-dimethyl-N-2-propenyl-), Dibromide / Q000633:toxicity; D002478:Cells, Cultured / Q000187:drug effects / Q000201:enzymology; D002619:Chemical Warfare Agents / Q000633:toxicity; D002642:Chick Embryo; D002800:Cholinesterase Inhibitors / Q000633:toxicity*; D003704:Dementia / Q000139:chemically induced / Q000201:enzymology / Q000503:physiopathology; D004785:Environmental Pollutants / Q000633:toxicity*; D005260:Female; D005822:Genetic Vectors / Q000502:physiology; D006706:Homeostasis / Q000187:drug effects / Q000235:genetics; D006801:Humans; D009457:Neuroglia / Q000187:drug effects* / Q000201:enzymology; D009474:Neurons / Q000187:drug effects* / Q000201:enzymology; D020734:Parkinsonian Disorders / Q000139:chemically induced / Q000201:enzymology / Q000503:physiopathology; D010575:Pesticides / Q000633:toxicity; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D011493:Protein Kinase C / Q000187:drug effects* / Q000378:metabolism; D012160:Retina / Q000187:drug effects* / Q000196:embryology / Q000201:enzymology; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology; D013312:Stress, Physiological / Q000139:chemically induced / Q000201:enzymology / Q000503:physiopathology; D014162:Transfection
chemical_list: D002619:Chemical Warfare Agents; D002800:Cholinesterase Inhibitors; D004785:Environmental Pollutants; D010575:Pesticides; D002093:Benzenaminium, 4,4'-(3-oxo-1,5-pentanediyl)bis(N,N-dimethyl-N-2-propenyl-), Dibromide; D011493:Protein Kinase C; D000110:Acetylcholinesterase