Profile of eicosanoids produced by human saphenous vein endothelial cells and the effect of dietary fatty acids.
Human saphenous vein endothelial cells (HSVECs) derived from primary cultures of adult human veins constitute an excellent in vitro model for studying human endothelial metabolism. In this study we report the (14)C-labelled prostanoid profile of HSVECs under resting and stimulated conditions and the effect of the n-3 polyunsaturated fatty acids eicosapentaenoic acid and docosahexaenoic acid on them. Results indicate that HSVECs while under resting conditions produce mainly prostaglandin F(2alpha)(PGF(2alpha)). After stimulation with calcium ionophore A23187, the cells were found to synthesise PGI(2), PGE(2)and PGF(2alpha) as major products and thromboxane B(2)and PGD(2)as minor products. Production of (14)C-labelled hydroxyeicosatetraenoic acids was not detected. Eicosapentaenoic acid was found to inhibit basal and stimulated prostanoid production whereas docosahexaenoic acid inhibited basal but strongly increased stimulated prostanoid production. These results may offer the basis for further studies aiming to investigate targets for pharmacological intervention in inflammatory conditions.
Urquhart
P
P
School of Pharmacy, University of Bradford, Richmond Road, Bradford BD7 1DP, UK.
Parkin
S M
SM
Nicolaou
A
A
D000001:Calcimycin / Q000494:pharmacology; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D004041:Dietary Fats / Q000494:pharmacology*; D015237:Dinoprost / Q000096:biosynthesis; D015232:Dinoprostone / Q000096:biosynthesis; D004281:Docosahexaenoic Acids / Q000494:pharmacology; D015777:Eicosanoids / Q000096:biosynthesis*; D015118:Eicosapentaenoic Acid / Q000494:pharmacology; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism*; D011464:Epoprostenol / Q000096:biosynthesis; D005227:Fatty Acids / Q000008:administration & dosage / Q000494:pharmacology*; D006801:Humans; D006893:Hydroxyeicosatetraenoic Acids / Q000096:biosynthesis; D007476:Ionophores / Q000494:pharmacology; D015230:Prostaglandin D2 / Q000096:biosynthesis; D012501:Saphenous Vein; D013929:Thromboxane B2 / Q000096:biosynthesis
D004041:Dietary Fats; D015777:Eicosanoids; D005227:Fatty Acids; D006893:Hydroxyeicosatetraenoic Acids; D007476:Ionophores; D004281:Docosahexaenoic Acids; D000001:Calcimycin; D013929:Thromboxane B2; D015118:Eicosapentaenoic Acid; D015237:Dinoprost; D011464:Epoprostenol; D015232:Dinoprostone; D015230:Prostaglandin D2