[Detection of anti-HIV-1/2 and HIV nucleic acid in population of non-remunerated blood donors in Shenzhen].
OBJECTIVE
To study the prevalent status of HIV-1 in population of non-remunerated blood donors in Shenzhen.


METHODS
46,095 non-remunerated blood donors were tested for anti-HIV-1/2 by ELISA. The donors of anti-HIV positive were further detected for HIV DNA from peripheral blood mononuclear cells (PMBCs) by nested-PCR and HIV RNA from plasma by RT-PCR. The partial genome of env of 2 blood donors were sequenced.


RESULTS
The anti-HIV-1 was tested positive in 7 of 46 095 voluntary blood donors and the positive rate was 0.015%. In these 7 non-remunerated blood donors of anti-HIV-1 positive,7 were positive for HIV DNA in PMBCs and 5 positive for HIV RNA in plasma. The sequence analysis showed that 2 donors were infected by HIV-1 subtype E strains.


CONCLUSIONS
There exists HIV-1 subtype E infection in population of non-remunerated blood donors in Shenzhen. It is essential to detect and monitor strictly the population of non-remunerated blood donors.
Ou Yang
L
L
Shenzhen Baoan Blood Center, Shenzhen 518101, China.
Huang
C
C
Chen
R
R
D000293:Adolescent; D000328:Adult; D001483:Base Sequence; D001782:Blood Donors; D002681:China / Q000453:epidemiology; D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics; D005260:Female; D006678:HIV / Q000235:genetics* / Q000276:immunology; D015483:HIV Antibodies / Q000097:blood*; D015658:HIV Infections / Q000453:epidemiology* / Q000276:immunology; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000097:blood*; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
D004279:DNA, Viral; D015483:HIV Antibodies; D012367:RNA, Viral