Inhibitory action of gemfibrozil on cholesterol absorption in rat intestine.
This study was designed to determine whether gemfibrozil inhibits intestinal lipid absorption. Male Sprague-Dawley rats received an oral dose of 30 mg gemfibrozil/kg body weight for 14 days. Mesenteric lymph cannulation was performed, and a lipid infusion containing 40 micromol/h (35.4 mg/h) of radiolabeled triolein and 2.74 micromol/h (1.06 mg/h) of radiolabeled cholesterol with the addition of 1 mg/h of gemfibrozil was infused intraduodenally at a rate of 3 ml/h for 8 h. The lymph was collected, and the radioactivity levels of the lumen and gut mucosa were measured after the infusion. Lymph cholesterol transport was depressed in gemfibrozil-treated rats, in terms of mass measurements as well as radioactivity in a lesser degree. More radioactive cholesterol remained in the proximal portion of the intestinal lumen and mucosa in the treated rats than in the control rats. More radioactive triglycerides also remained in the proximal intestinal lumen of treated rats, although no difference in lymphatic triglyceride transport was observed between the groups. A significant portion of the radioactive cholesterol remained in the lumen in the gemfibrozil-treated rats. Gemfibrozil increased biliary cholesterol excretion. Thus, this study shows that gemfibrozil inhibits cholesterol absorption in rat intestine.
Umeda
Y
Y
Department of Pediatrics, Showa General Hospital, Japan.
Kako
Y
Y
Mizutani
K
K
Iikura
Y
Y
Kawamura
M
M
Seishima
M
M
Hayashi
H
H
D000818:Animals; D001646:Bile / Q000378:metabolism; D002250:Carbon Radioisotopes; D002784:Cholesterol / Q000008:administration & dosage / Q000378:metabolism*; D002788:Cholesterol Esters / Q000378:metabolism; D004386:Duodenum / Q000187:drug effects / Q000378:metabolism; D015248:Gemfibrozil / Q000008:administration & dosage / Q000494:pharmacology*; D000960:Hypolipidemic Agents / Q000008:administration & dosage / Q000494:pharmacology*; D007408:Intestinal Absorption / Q000187:drug effects*; D007413:Intestinal Mucosa / Q000378:metabolism; D007700:Kinetics; D008196:Lymph / Q000502:physiology; D008208:Lymphatic System / Q000378:metabolism; D008297:Male; D008643:Mesentery; D051381:Rats; D017207:Rats, Sprague-Dawley; D014280:Triglycerides / Q000378:metabolism; D014304:Triolein / Q000008:administration & dosage; D014316:Tritium
D002250:Carbon Radioisotopes; D002788:Cholesterol Esters; D000960:Hypolipidemic Agents; D014280:Triglycerides; D014316:Tritium; D014304:Triolein; D002784:Cholesterol; D015248:Gemfibrozil