[Conjugation and identification of the surface protein antigen P1 from streptococcus mutans MT6R with the cholera toxin B subunit and procholeragenoid].
To make an effective antigen to prevent dental caries, the surface protein antigen P1(Ag I/II) was purified and identified by the rabbit anti-Ag I/II serum and was covalently conjugated to CTB and PCG by SPDP respectively. GM1-ELISA showed that both the conjugated P1-CTB and P1-PCG possess the ability to bind GM1 specifically without losing the antigenicity of P1. This results showed that the conjugated antigens could be used in the immune prevention against dental caries.
Li
F
F
College of Stomatology, West China University of Medical Sciences.
Luo
Z
Z
Zhang
J
J
D000942:Antigens, Bacterial / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000302:isolation & purification; D002772:Cholera Toxin; D003432:Cross-Linking Reagents; D000922:Immunotoxins; D008562:Membrane Glycoproteins; D010446:Peptide Fragments; D013295:Streptococcus mutans / Q000737:chemistry*; D013388:Succinimides
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D003432:Cross-Linking Reagents; D000922:Immunotoxins; D008562:Membrane Glycoproteins; D010446:Peptide Fragments; C055764:S-layer proteins; D013388:Succinimides; C018151:N-succinimidyl 3-(2-pyridyldithio)propionate; C050310:cholera toxin, B subunit (50-64); D002772:Cholera Toxin