title: Effects of recording media composition on the responses of Nafion-coated carbon fiber microelectrodes measured using high-speed chronoamperometry.
abstract: The present study concerns methodological issues of electrochemical recordings using Nafion-coated 30 microm diameter single carbon fiber microelectrodes for high-speed chronoamperometric measurements of biogenic amines. First, the single carbon fiber microelectrodes were coated with Nafion and dried at 85 vs. 200 degrees C and their recording properties were determined. Second, the effects of shifts in solution pH, ionic strength, changes in recording solution levels of Ca(2+) or Mg(2+) and temperature on the recording characteristics and sensitivity of Nafion-coated high temperature dried (200 degrees C) single carbon fiber microelectrodes for measures of dopamine were studied. These studies showed that the high temperature drying of the Nafion produced a microelectrode with better recording properties: higher selectivity for cations versus anions, increased differences between the reduction and oxidation current ratios for the identification of dopamine versus serotonin, and more rapid response times. In addition, these studies demonstrated that the chronoamperometric recordings were insensitive to small changes in pH and divalent cations such as Ca(2+) or Mg(2+). However, increases in ionic strength decreased the sensitivity of the microelectrodes, while increases in temperature produced increases in the sensitivity of the microelectrodes for biogenic amines. These data support that Nafion-coated high temperature (200 degrees C) dried microelectrodes have enhanced recording properties as compared to microelectrodes, which are coated with Nafion and dried at 85 degrees C. In addition, high-speed chronoamperometric recordings of biogenic amines are not affected by solution changes in divalent cations (Ca(2+) or Mg(2+)).
authors.affiliation: Department of Anatomy and Neurobiology and the Morris K. Udall Parkinson's Disease Research Center of Excellence, 306 Davis Mills Bldg., University of Kentucky, Chandler Medical Center, 800 Rose Street, Lexington, KT 40536-0098, USA. gregg@pop.uky.edu
authors.forename: G A
authors.initials: GA
authors.lastname: Gerhardt
authors.forename: A F
authors.initials: AF
authors.lastname: Hoffman
mesh_terms: D000818:Animals; D001679:Biogenic Amines / Q000032:analysis*; D002021:Buffers; D002118:Calcium / Q000378:metabolism / Q000494:pharmacology; D002244:Carbon; D000077482:Carbon Fiber; D004594:Electrophysiology / Q000379:methods*; D005110:Extracellular Space / Q000378:metabolism; D005465:Fluorocarbon Polymers / Q000592:standards; D006863:Hydrogen-Ion Concentration; D007477:Ions / Q000378:metabolism / Q000494:pharmacology; D008274:Magnesium / Q000378:metabolism / Q000494:pharmacology; D008839:Microelectrodes / Q000639:trends*; D051381:Rats; D013696:Temperature
chemical_list: D001679:Biogenic Amines; D002021:Buffers; D000077482:Carbon Fiber; D005465:Fluorocarbon Polymers; D007477:Ions; C040402:perfluorosulfonic acid; D002244:Carbon; D008274:Magnesium; D002118:Calcium