title: [Clinical algorithm for cerebrospinal fluid test of 14-3-3 protein in diagnosis of Creutzfeldt-Jacob disease].
abstract: OBJECTIVE
  To study whether an algorithm that includes additional diagnostic information could increase the specificity of the 14-3-3 protein testing in patients suspected to suffer from Creutzfeldt-Jakob disease (CJD).
  
  
  METHODS
  The development of a diagnostic algorithm.
  
  
  METHODS
  The 14-3-3 protein was tested in the cerebrospinal fluid from 69 consecutive patients suspected of having CJD. On the basis of a former study and literature research, a diagnostic algorithm was constructed, which restricted the indication for performing the 14-3-3 protein test.
  
  
  RESULTS
  By taking into consideration the findings of neuroimaging and routine cerebrospinal fluid examination prior to 14-3-3 testing, the specificity increased to 97% (95%-CI: 85.5-99.9) thus changing the prior probability of having CJD of 35% to a posterior probability of 75-100%, in the case of a positive test result.
  
  
  CONCLUSIONS
  Determining the presence of 14-3-3 protein is a highly sensitive and specific marker for sporadic CJD when used in combination with imaging and cerebrospinal fluid examination.
authors.affiliation: Afd. Neurologie, Academisch Medisch Centrum, Postbus 22700, 1100 DE Amsterdam. a.w.lemstra@amc.uva.nl
authors.forename: A W
authors.initials: AW
authors.lastname: Lemstra
authors.forename: M
authors.initials: M
authors.lastname: van Meegen
authors.forename: F
authors.initials: F
authors.lastname: Baas
authors.forename: W A
authors.initials: WA
authors.lastname: van Gool
mesh_terms: D048948:14-3-3 Proteins; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000465:Algorithms; D015415:Biomarkers / Q000134:cerebrospinal fluid; D002648:Child; D007562:Creutzfeldt-Jakob Syndrome / Q000134:cerebrospinal fluid* / Q000175:diagnosis*; D003937:Diagnosis, Differential; D005260:Female; D006801:Humans; D015151:Immunoblotting; D008297:Male; D008875:Middle Aged; D012680:Sensitivity and Specificity; D014446:Tyrosine 3-Monooxygenase / Q000134:cerebrospinal fluid*
chemical_list: D048948:14-3-3 Proteins; D015415:Biomarkers; D014446:Tyrosine 3-Monooxygenase