Detection of anti-listeriolysin O and Listeria monocytogenes in experimentally infected buffaloes (Bubalus bubalis).
The kinetics of antibody production against listeriolysin O (ALLO) and the recovery pattern of Listeria monocytogenes from bacteriological samples were studied following oral infection of buffalo calves with 3 x 10(9) cells each of pathogenic L. monocytogenes. Antibodies to LLO appeared by 7-10 days post infection (PI), with a shallow peak between days 16 and 36 PI, when tested by indirect plate-ELISA. The titres of ALLO in all the animals then declined slowly but remained detectable up to day 70 PI. In dot-ELISA, ALLO could be detected by days 5 to 7 PI, and with higher titres than with the plate-ELISA. The pathogen was recovered at low rates as ALLO first appeared but was absent in the faecal, nasal and blood cultures as production of ALLO peaked.
Chaudhari
S P
SP
Division of Veterinary Public Health, Indian Veterinary Research Institute, Izatnagar, UP, India. icar@goa.goa.nic.in
Malik
S V
SV
Rekha
G B
GB
Barbuddhe
S B
SB
D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis*; D001427:Bacterial Toxins; D001431:Bacteriological Techniques; D002020:Buffaloes; D015169:Colony Count, Microbial; D006360:Heat-Shock Proteins / Q000276:immunology* / Q000302:isolation & purification; D006460:Hemolysin Proteins / Q000276:immunology* / Q000302:isolation & purification; D007700:Kinetics; D008089:Listeria monocytogenes / Q000254:growth & development / Q000276:immunology*; D008088:Listeriosis / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*
D000907:Antibodies, Bacterial; D001427:Bacterial Toxins; D006360:Heat-Shock Proteins; D006460:Hemolysin Proteins; C038705:hlyA protein, Listeria monocytogenes