[A method for preparation of carbon fiber electrode].
Carbon fiber electrode (CFE) can be used in detection of exocytosis of single neuron or endocrine cell. A simplified method for preparation of CFE, as described in the present paper, greatly ensures the coordination and success of the preparation. Such CFEs have a low noise level. Exocytosis of rat adrenal chromaffin cells was investigated in the clarification of the effect of MPP+ on transmitter release. It is found that exocytosis was not stimulated by MPP+ and the high K+ induced secretion was not changed by MPP+. These observations suggest that the previously reported elevation of dopamine content in the surrounding histosolution of DA neuron is probably resulted from blockage of the re-uptake of DA by MPP+.
Cai
D
D
Institute of Biophysics and Biochemistry, Huazhong University of Science and Technology, Wuhan, 430074.
Qu
A L
AL
Wang
X M
XM
Zhou
Z
Z
Xu
J H
JH
Han
J S
JS
D015655:1-Methyl-4-phenylpyridinium / Q000494:pharmacology*; D000313:Adrenal Medulla / Q000378:metabolism; D000818:Animals; D002244:Carbon; D019439:Chromaffin Cells / Q000378:metabolism*; D004563:Electrochemistry; D004566:Electrodes / Q000145:classification*; D005089:Exocytosis / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar
D002244:Carbon; D015655:1-Methyl-4-phenylpyridinium