title: Post-insult activity is a major cause of delayed neuronal death in organotypic hippocampal slices exposed to glutamate.
abstract: We investigated the pathophysiological mechanisms of glutamate-induced delayed neuronal damage in rat hippocampal slice cultures [Stoppini et al. (1991) J. Neurosci. Methods 37, 173-182], with propidium iodide as a marker of cell death. Exposure of the cultures to growth medium containing 10 mM glutamate for 30 min resulted in a slowly developing degeneration of hippocampal principal cells, starting from the medial end of the CA1 region and reaching the dentate gyrus by 48 h. By 24 h, most pyramidal cells in CA1 were damaged. An acute phase of degeneration preceded the delayed damage at 2-6 h, affecting cells in a spatially diffuse manner. When tetrodotoxin (0.5 microM) was present during the glutamate insult, a marked protection (mean 57%, P<0.001) of the CA1 damage was observed. Rather strikingly, when tetrodotoxin was applied immediately following or even with a delay of 30 min after the insult, a similar amount of protection was achieved. In field recordings carried out after the insult, the glutamate-treated slices exhibited spontaneously occurring negative shifts with a duration of 1-10 s and an amplitude of up to 400 microV in the CA3 region, whereas the control slices were always quiescent. Taken together, the results suggest that post-insult neuronal network activity, rather than the direct action of exogenous glutamate, is a major cause of delayed CA1 pyramidal cell death in the organotypic slices. These observations may have implications in the design of neuroprotective strategies for the treatment of brain traumas which are accompanied by delayed and/or distal neuronal damage.
authors.affiliation: Department of Biosciences, University of Helsinki, FIN-00014, Helsinki, Finland. hannele.lahtinen@helsinki.fi
authors.forename: H
authors.initials: H
authors.lastname: Lahtinen
authors.forename: A M
authors.initials: AM
authors.lastname: Autere
authors.forename: P
authors.initials: P
authors.lastname: Paalasmaa
authors.forename: S E
authors.initials: SE
authors.lastname: Lauri
authors.forename: K
authors.initials: K
authors.lastname: Kaila
mesh_terms: D000200:Action Potentials / Q000187:drug effects / Q000502:physiology; D000818:Animals; D001930:Brain Injuries / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D002545:Brain Ischemia / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D016923:Cell Death / Q000187:drug effects / Q000502:physiology*; D004827:Epilepsy / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D018698:Glutamic Acid / Q000378:metabolism / Q000633:toxicity*; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism / Q000503:physiopathology; D009410:Nerve Degeneration / Q000139:chemically induced / Q000378:metabolism* / Q000503:physiopathology; D009415:Nerve Net / Q000187:drug effects / Q000378:metabolism / Q000503:physiopathology; D009498:Neurotoxins / Q000378:metabolism / Q000633:toxicity*; D009924:Organ Culture Techniques; D017966:Pyramidal Cells / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D013779:Tetrodotoxin / Q000494:pharmacology; D013997:Time Factors
chemical_list: D009498:Neurotoxins; D018698:Glutamic Acid; D013779:Tetrodotoxin