Functional analysis of the LACERATA gene of Arabidopsis provides evidence for different roles of fatty acid omega -hydroxylation in development.
We describe lacerata (lcr) mutants of Arabidopsis, which display various developmental abnormalities, including postgenital organ fusions, and report cloning of the LCR gene by using the maize transposon Enhancer/Suppressor-mutator (En/Spm). The pleiotropic mutant phenotype could be rescued by genetic complementation of lcr mutants with the wild-type LCR gene. The LCR gene encodes a cytochrome P450 monooxygenase, CYP86A8, which catalyzes omega-hydroxylation of fatty acids ranging from C12 to C18:1, as demonstrated by expression of the gene in yeast. Although palmitic and oleic acids were efficient substrates for LCR, 9,10-epoxystearate was not metabolized. Taken together with previous studies, our findings indicate that LCR-dependent omega-hydroxylation of fatty acids could be implicated in the biosynthesis of cutin in the epidermis and in preventing postgenital organ fusions. Strikingly, the same pathway seems to control trichome differentiation, the establishment of apical dominance, and senescence in plants.
Wellesen
K
K
Max-Planck-Institut für Züchtungsforschung, Cologne, Germany.
Durst
F
F
Pinot
F
F
Benveniste
I
I
Nettesheim
K
K
Wisman
E
E
Steiner-Lange
S
S
Saedler
H
H
Yephremov
A
A
D000483:Alleles; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D029681:Arabidopsis Proteins; D001483:Base Sequence; D002454:Cell Differentiation; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics* / Q000502:physiology; D004251:DNA Transposable Elements / Q000235:genetics; D005227:Fatty Acids / Q000378:metabolism*; D017343:Genes, Plant; D005816:Genetic Complementation Test; D006900:Hydroxylation; D008563:Membrane Lipids / Q000096:biosynthesis; D006899:Mixed Function Oxygenases / Q000235:genetics* / Q000502:physiology; D008969:Molecular Sequence Data; D009024:Morphogenesis; D010641:Phenotype; D019441:Plant Epidermis / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000502:physiology; D030821:Plants, Genetically Modified; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D015398:Signal Transduction
D029681:Arabidopsis Proteins; D004251:DNA Transposable Elements; D005227:Fatty Acids; D008563:Membrane Lipids; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; C000521:cutin; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases; C434730:CYP86A8 protein, Arabidopsis