A novel Xenopus acetyltransferase with a dynamic expression in early development.
We have isolated a novel acetyltransferase from Xenopus laevis, named Xat-1. Xat-1 cDNA encodes a predicted protein of 846 amino acids that contains tetratricopeptide repeat (TPR) domains mediating protein-protein interactions and a bipartite nuclear localization signal (NLS). Its apparent molecular mass of 98.8 kDa was determined by SDS-PAGE analysis of Xat-1 recombinant protein in vitro translated in rabbit reticulocyte lysate. Xat-1 is homologous to N-terminal acetyltransferase 1 (NAT1), a gene that was originally discovered in yeast. Furthermore, it has many orthologs from human, mouse, Drosophila, C. elegans, and even Arabidopsis, thereby suggesting that these constitute a novel acetyltransferase family whose functions have been not examined. Xat-1 transcripts are expressed at relatively constant levels throughout early embryonic stages. They also exhibit dynamic expression pattern in brain, somites, branchial arches, pronephros, and otic vesicles.
Choi
S C
SC
Division of Molecular and Life Sciences, Pohang University of Science and Technology, San 31 Hyoja-Dong, Pohang, Kyungbuk, 790-784, Republic of Korea.
Chang
J Y
JY
Han
J K
JK
D000123:Acetyltransferases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000196:embryology / Q000201:enzymology; D001934:Branchial Region / Q000196:embryology / Q000201:enzymology; D002474:Cell-Free System; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D007758:Ear, Inner / Q000196:embryology / Q000201:enzymology; D004591:Electrophoresis, Polyacrylamide Gel; D018507:Gene Expression Regulation, Developmental; D017403:In Situ Hybridization; D007668:Kidney / Q000196:embryology / Q000201:enzymology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D005810:Multigene Family; D009928:Organ Specificity / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D019170:Somites / Q000201:enzymology; D029867:Xenopus Proteins; D014982:Xenopus laevis
D018076:DNA, Complementary; D029867:Xenopus Proteins; D000123:Acetyltransferases; C434048:NAA15 protein, Xenopus; C061313:protein N-terminal acetyltransferase