[Quantitative study on the expression of mRNA for TGF-beta and collagenase (MMP-1), tissue metalloproteinase inhibitor-1 (TIMP-1) in hypertrophic scar].
OBJECTIVE
To investigate the expression of mRNA for TGF-beta, MMP-1 and TIMP-1 and their effects on the formation of hypertrophic scar (HTS).


METHODS
In situ hybridization and image analysis were used in this study.


RESULTS
The expressions of mRNA for TGF-beta and TIMP-1 in HTS was greatly increased compared with those of normal skin. The average integral optical density of mRNA for TGF-beta and TIMP-1 increased by 8 and 7 folds (P < 0.001) related to normal skin and the expression of them showed highly positive correlation.


CONCLUSIONS
The findings suggest that TGF-beta promotes the expression of TIMP-1 and may be a possible factor for hypertrophic scar formation.
Wu
J
J
Department of Physical Medicine and Rehabilitation, Southwest Hospital, Third Military Medical University, Chongqing 400038, PR China.
Chen
D
D
Wu
Z
Z
D000293:Adolescent; D000328:Adult; D002648:Child; D017439:Cicatrix, Hypertrophic / Q000378:metabolism*; D017364:Collagenases / Q000096:biosynthesis / Q000235:genetics; D005260:Female; D006801:Humans; D008297:Male; D020781:Matrix Metalloproteinase 1 / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000096:biosynthesis* / Q000235:genetics; D016212:Transforming Growth Factor beta / Q000096:biosynthesis* / Q000235:genetics
D012333:RNA, Messenger; D019715:Tissue Inhibitor of Metalloproteinase-1; D016212:Transforming Growth Factor beta; D017364:Collagenases; D020781:Matrix Metalloproteinase 1