In vitro evaluation of the heparin-coated Gyro C1E3 blood pump.
The blood-contacting surface, mainly composed of polycarbonate, of the Gyro C1E3 pump was modified using plasma glow discharge to introduce a carboxyl functional group, coated with a base layer of polyethyleneimine as a linker, and coupled with heparin by multi-ionic binding to enhance blood compatibility. A relative surface content of 3.7% sulfur, which demonstrated heparin immobilization on the polycarbonate substrate, was observed on x-ray photoelectron spectroscopy, and a initial bioactivity of approximately 88.5 +/- 7.3 mIU/cm2 was obtained by the chromogenic method for antifactor Xa assay. Furthermore, in vitro observation of platelet and fibrin adherence using bovine blood under dynamic flow conditions for 6 h revealed that the multi-ionically heparinized Gyro C1E3 had significantly stronger antithrombogenecity than the noncoated original type which was evaluated as a good hemocompatible blood pump for clinical use. Not only the Gyro C1E3 but also the ionically heparin-coated Gyro pump are expected to be thromboresistant in clinical use.
Muramatsu
K
K
Research and Development Department, Bioceram Division, Kyocera Corporation, 10-1, Kawai, Gamo-cho, Shiga 529-1595, Japan. kazuaki-muramatsu@kyocera.co.jp
Masuoka
T
T
Fujisawa
A
A
D001777:Blood Coagulation / Q000502:physiology; D002315:Cardiopulmonary Bypass / Q000295:instrumentation; D020099:Coated Materials, Biocompatible; D004867:Equipment Design; D004869:Equipment Safety; D006353:Heart-Assist Devices; D006355:Heart-Lung Machine; D006493:Heparin / Q000737:chemistry*; D006801:Humans; D011076:Polycarboxylate Cement / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013499:Surface Properties; D013927:Thrombosis / Q000517:prevention & control
D020099:Coated Materials, Biocompatible; D011076:Polycarboxylate Cement; C005506:polycarbonate; D006493:Heparin