title: Essential role of Drosophila Hdac1 in homeotic gene silencing.
abstract: Deacetylation of the N-terminal tails of core histones plays a crucial role in gene silencing. Rpd3 and Hda1 represent two major types of genes encoding trichostatin A-sensitive histone deacetylases. Although they have been widely found, their cellular and developmental roles remain to be elucidated in metazoa. We show that Drosophila Hdac1, an Rpd3-type gene, interacts cooperatively with Polycomb group repressors in silencing the homeotic genes that are essential for axial patterning of body segments. The biochemical copurification and cytological colocalization of HDAC1 and Polycomb group repressors strongly suggest that HDAC1 is a component of the silencing complex for chromatin modification on specific regulatory regions of homeotic genes.
authors.affiliation: Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan, Republic of China.
authors.forename: Y L
authors.initials: YL
authors.lastname: Chang
authors.forename: Y H
authors.initials: YH
authors.lastname: Peng
authors.forename: I C
authors.initials: IC
authors.lastname: Pan
authors.forename: D S
authors.initials: DS
authors.lastname: Sun
authors.forename: B
authors.initials: B
authors.lastname: King
authors.forename: D H
authors.initials: DH
authors.lastname: Huang
mesh_terms: D000818:Animals; D002874:Chromosome Mapping; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000196:embryology / Q000235:genetics* / Q000378:metabolism; D020868:Gene Silencing; D005801:Genes, Homeobox / Q000235:genetics; D017344:Genes, Insect; D056284:Histone Deacetylase 1; D056464:Histone Deacetylase 2; D006655:Histone Deacetylases / Q000502:physiology*; D006657:Histones / Q000378:metabolism; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D046911:Macromolecular Substances; D009024:Morphogenesis; D005810:Multigene Family; D063150:Polycomb Repressive Complex 1; D012097:Repressor Proteins / Q000502:physiology
chemical_list: D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D006657:Histones; D019476:Insect Proteins; D046911:Macromolecular Substances; C066770:Pc protein, Drosophila; D012097:Repressor Proteins; C071050:Psc protein, Drosophila; D063150:Polycomb Repressive Complex 1; C110550:HDAC1 protein, human; C400300:HDAC4 protein, human; D056284:Histone Deacetylase 1; D056464:Histone Deacetylase 2; D006655:Histone Deacetylases