title: Flow-mediated vasodilation is not impaired when HDL-cholesterol is lowered by substituting carbohydrates for monounsaturated fat.
abstract: Low-fat diets, in which carbohydrates replace some of the fat, decrease serum cholesterol. This decrease is due to decreases in LDL-cholesterol but in part to possibly harmful decreases in HDL-cholesterol. High-oil diets, in which oils rich in monounsaturated fat replace some of the saturated fat, decrease serum cholesterol mainly through LDL-cholesterol. We used these two diets to investigate whether a change in HDL-cholesterol would change flow-mediated vasodilation, a marker of endothelial function. We fed thirty-two healthy volunteers two controlled diets in a weeks' randomised cross-over design to eliminate variation in changes due to differences between subjects. The low-fat diet contained 59.7 % energy (en%) as carbohydrates and 25.7 en% as fat (7.8 en% as monounsaturates); the oil-rich diet contained 37.8 en% as carbohydrates and 44.4 en% as fat (19.3 en% as monounsaturates). Average (sd) serum HDL-cholesterol after the low-fat diet was 0.21 (sd 0.12) mmol/l (8.1 mg/dl) lower than after the oil-rich diet. Serum triacylglycerols were 0.22 (sd 0.28) mmol/l (19.5 mg/dl) higher after the low-fat diet than after the oil-rich diet. Serum LDL and homocysteine concentrations remained stable. Flow-mediated vasodilation was 4.8 (SD 2.9) after the low-fat diet and 4.1 (SD 2.7) after the oil-rich diet (difference 0.7 %; 95 % CI -0.6, 1.9). Thus, although the low-fat diet produced a lower HDL-cholesterol than the high-oil diet, flow-mediated vasodilation, an early marker of cardiovascular disease, was not impaired.
authors.affiliation: Division of Human Nutrition and Epidemiology, Wageningen University, Wageningen, the Netherlands. nicole.deroos@staff.nutepi.wau.nl
authors.forename: N M
authors.initials: NM
authors.lastname: de Roos
authors.forename: M L
authors.initials: ML
authors.lastname: Bots
authors.forename: E
authors.initials: E
authors.lastname: Siebelink
authors.forename: E
authors.initials: E
authors.lastname: Schouten
authors.forename: M B
authors.initials: MB
authors.lastname: Katan
mesh_terms: D000328:Adult; D008076:Cholesterol, HDL / Q000097:blood*; D008078:Cholesterol, LDL / Q000097:blood*; D018592:Cross-Over Studies; D004040:Dietary Carbohydrates / Q000008:administration & dosage*; D004042:Dietary Fats, Unsaturated / Q000008:administration & dosage*; D005260:Female; D006710:Homocysteine / Q000097:blood; D006801:Humans; D008297:Male; D008875:Middle Aged; D012039:Regional Blood Flow / Q000502:physiology; D014280:Triglycerides / Q000097:blood; D014664:Vasodilation / Q000502:physiology*
chemical_list: D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D004040:Dietary Carbohydrates; D004042:Dietary Fats, Unsaturated; D014280:Triglycerides; D006710:Homocysteine