Stimulatory effect of D-ephedrine on beta3-adrenoceptors in adipose tissue of rats.
The effect of ephedrine on beta3-adrenoceptos (beta3-AR) was studied in the isolated adipose tissue of Wistar rat. Incubation with D-ephedrine (0.1-10 microM) induced a concentration-dependent decrease of uptake of [14C]-deoxy-D-glucose into white adipose tissues (WAT). The inhibitory effect of D-ephedrine was potentiated by BRL 37344, the agonist of beta3-AR and concentration-dependently inhibited by SR 59230A, the selective antagonist of beta3-AR. The action of D-ephedrine on beta3-AR was further blocked by the antibodies for beta3-AR, but not the immunoglobulin. in a concentration-dependent manner. Moreover, D-ephedrine increased glycerol release from the isolated brown adipose tissues (BAT) and this action was also abolished by SR 59230A at concentration sufficient to block beta3-AR. Thus, these results suggest that D-ephedrine has the ability to activate beta3-AR both in WAT and BAT of Wistar rats in vitro.
Cheng
J T
JT
Department of Pharmacology, College of Medicine, National Cheng Kung University, Tainan, Taiwan. jtcheng@mail.ncku.edu
Liu
I M
IM
Yen
S T
ST
Juang
S W
SW
Liu
T P
TP
Chan
P
P
D000273:Adipose Tissue / Q000166:cytology / Q000378:metabolism*; D002001:Adipose Tissue, Brown / Q000378:metabolism; D000318:Adrenergic beta-Agonists / Q000494:pharmacology*; D000319:Adrenergic beta-Antagonists / Q000494:pharmacology; D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D003847:Deoxyglucose / Q000493:pharmacokinetics; D004357:Drug Synergism; D004809:Ephedrine / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D004983:Ethanolamines / Q000494:pharmacology; D005990:Glycerol / Q000378:metabolism; D008297:Male; D011412:Propanolamines / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D011943:Receptors, Adrenergic, beta / Q000187:drug effects* / Q000276:immunology
C097869:3-(2-ethylphenoxy)-1-(1,2,3,4-tetrahydronaphth-1-ylamino)-2-propanol oxalate; D000318:Adrenergic beta-Agonists; D000319:Adrenergic beta-Antagonists; D000906:Antibodies; D004983:Ethanolamines; D011412:Propanolamines; D011943:Receptors, Adrenergic, beta; C057368:BRL 37344; D003847:Deoxyglucose; D004809:Ephedrine; D005990:Glycerol