title: Accuracy and precision of dual-energy X-ray absorptiometry for body composition measurements in rhesus monkeys.
abstract: Accuracy of body composition measurements by dual-energy X-ray absorptiometry (DXA) was compared with direct chemical analysis in 10 adult rhesus monkeys. DXA was highly correlated (r-values > 0.95) with direct analyses of body fat mass (FM), lean mass (LM) and lumbar spine bone mineral content (BMC). DXA measurements of total body BMC were not as strongly correlated (r-value = 0.58) with total carcass ash content. DXA measurements of body FM, LM and lumbar spine BMC were not different from data obtained by direct analyses (P-values > 0.30). In contrast, DXA determinations of total BMC (TBMC) averaged 15%, less than total carcass ash measurements (P = 0.002). In conclusion, this study confirms the accurate measurement of fat and lean tissue mass by DXA in rhesus monkeys. DXA also accurately measured lumbar spine BMC but underestimated total body BMC as compared with carcass ash determinations.
authors.affiliation: Nutritional and Molecular Physiology Unit, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, USA.
authors.forename: A
authors.initials: A
authors.lastname: Black
authors.forename: E M
authors.initials: EM
authors.lastname: Tilmont
authors.forename: D J
authors.initials: DJ
authors.lastname: Baer
authors.forename: W V
authors.initials: WV
authors.lastname: Rumpler
authors.forename: D K
authors.initials: DK
authors.lastname: Ingram
authors.forename: G S
authors.initials: GS
authors.lastname: Roth
authors.forename: M A
authors.initials: MA
authors.lastname: Lane
mesh_terms: D015502:Absorptiometry, Photon / Q000379:methods / Q000662:veterinary*; D000273:Adipose Tissue; D000818:Animals; D001823:Body Composition; D015519:Bone Density; D008159:Lumbar Vertebrae / Q000737:chemistry; D008253:Macaca mulatta / Q000502:physiology; D008297:Male; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity