title: Characterization by matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometry of the major photoproducts of temoporfin (m-THPC) and bacteriochlorin (m-THPBC).
abstract: The photobleaching of 5,10,15,20-tetrakis(m-hydroxyphenyl)chlorin (temoporfin, m-THPC) and 5,10,15,20-tetrakis(m-hydroxyphenyl)bacteriochlorin (bacteriochlorin, m-THPBC) was studied in ethanol-water (1 : 99, v/v) and in physiological medium (phosphate-buffered saline, PBS) with or without fetal calf serum (FCS). m-THPC solution was irradiated with the laser radiation of 650 nm, whereas m-THPBC solution underwent two consecutive irradiations at 532 and 650 nm. The photoproducts were characterized by UV-visible absorption spectrophotometry and by matrix-assisted laser desorption/ionization (MALDI) coupled with Fourier transform ion cyclotron resonance mass spectrometry (FTICRMS). Independent of the solvent used, the phototransformation of either photosensitizer yielded the formation of 5,10,15,20-tetrakis (m-hydroxyphenyl)porphyrin (m-THPP) through a major dehydrogenation process.
authors.affiliation: Laboratoire de Spectrométrie de Masse et de Chimie Laser, 1 Bd Arago, 57078 Metz Cedex 3, France.
authors.forename: M
authors.initials: M
authors.lastname: Angotti
authors.forename: B
authors.initials: B
authors.lastname: Maunit
authors.forename: J F
authors.initials: JF
authors.lastname: Muller
authors.forename: L
authors.initials: L
authors.lastname: Bezdetnaya
authors.forename: F
authors.initials: F
authors.lastname: Guillemin