title: Genetic transformation of Trametes versicolor to phleomycin resistance with the dominant selectable marker shble.
abstract: We have developed a stable, DNA-mediated transformation system for the white-rot basidiomycete Trametes versicolor based on the dominant selectable marker shble (phleomycin resistance). We employed a vector containing the selectable marker under control of expression sequences from the basidiomycete Schizophyllum commune and a polyethylene glycol/ CaCl2 protoplast-fusion technique to introduce the transforming DNA. This transformation system generated stable phleomycin-resistant transformants at a frequency of four to seven transformants/microg of transforming DNA.
authors.affiliation: Department of Biological Sciences, Central Connecticut State University, New Britain 06050, USA. bartholomewk@ccsu.edu
authors.forename: K
authors.initials: K
authors.lastname: Bartholomew
authors.forename: G
authors.initials: G
authors.lastname: Dos Santos
authors.forename: T
authors.initials: T
authors.lastname: Dumonceaux
authors.forename: T
authors.initials: T
authors.lastname: Charles
authors.forename: F
authors.initials: F
authors.lastname: Archibald
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001487:Basidiomycota / Q000187:drug effects / Q000235:genetics*; D015139:Blotting, Southern; D025141:Drug Resistance, Fungal; D010692:Phleomycins / Q000494:pharmacology*; D014170:Transformation, Genetic
chemical_list: D000900:Anti-Bacterial Agents; D010692:Phleomycins