[Preventive effect of endothelin-1 mRNA antisense oligonucleotide on acute myocardial ischemic arrhythmia in rats].
Acute myocardial ischemia was induced by occlusion of the anterior descending of left coronary artery (LAD) in rats; the resultant arrhythmia in 1 h after LAD occlusion was evaluated. In order to prevent expression of endothelin-1 mRNA, human endothelin-1 mRNA antisense oligonucleotide (ET-1 AS-ODN) was intravenously injected 2 h before LAD occlusion. The effect of AS-ODN on plasma ET-1 concentration and the acute ischemic arrhythmia were observed. The results showed that plasma ET-1 was significantly decreased in rats pretreated with AS-ODN, and both the incidence and severity of the acute ischemic arrhythmia were decreased dose-dependently as compared with normal saline control and sense oligonucleotide control, indicating that ET-1 AS-ODN could prevent acute myocardial ischemic arrhythmia and that endogenous endothelin-1 may play an important role in the development of acute ischemic arrhythmia in rats.
Lin
L
L
Department of Physiology, Second Military Medical University, Shanghai 200433.
Yuan
W J
WJ
Chu
X L
XL
Xu
H
H
Li
L
L
Ren
A J
AJ
D000818:Animals; D001145:Arrhythmias, Cardiac / Q000209:etiology / Q000378:metabolism* / Q000517:prevention & control; D019332:Endothelin-1 / Q000096:biosynthesis* / Q000235:genetics / Q000494:pharmacology; D008297:Male; D017202:Myocardial Ischemia / Q000150:complications*; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley
D019332:Endothelin-1; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger