title: Assembly of pore proteins on gold electrodes.
abstract: Protein-protein interactions at the cell surface are important in the activity of bacterial toxins such as colicins. We have developed methods to study these events using tethered lipid bilayers, which can be probed by impedance spectroscopy and surface plasmon resonance. Recently we have attached the receptor proteins directly to gold electrodes and this offers new possibilities for measuring protein-protein interactions on solid supports.
authors.affiliation: School of Biochemistry and Genetics, The Medical School, Newcastle upon Tyne, NE2 4HH, UK.
authors.forename: Q
authors.initials: Q
authors.lastname: Hong
authors.forename: S
authors.initials: S
authors.lastname: Terrettaz
authors.forename: W P
authors.initials: WP
authors.lastname: Ulrich
authors.forename: H
authors.initials: H
authors.lastname: Vogel
authors.forename: J H
authors.initials: JH
authors.lastname: Lakey
mesh_terms: D001665:Binding Sites; D003087:Colicins / Q000737:chemistry / Q000378:metabolism; D004566:Electrodes; D006046:Gold; D008051:Lipid Bilayers; D018272:Porins / Q000096:biosynthesis* / Q000737:chemistry / Q000378:metabolism
chemical_list: D003087:Colicins; D008051:Lipid Bilayers; C109237:OmpF protein; D018272:Porins; D006046:Gold