[Analysis of main pigments and other ingredients in lac color product].
The contents of the main pigments and other ingredients in commercial lac color products were determined by HPLC using an RP-18 column with 0.1 mol/L citric acid buffer solution-methanol (16:5) as the mobile phase, and a photodiode array (PDA) detector set at 280 nm and 490 nm. The main pigments were confirmed by PDA and electrospray ionization mass spectrometry. Laccaic acids A, B, C and E were detected in all lac color products, and the ratio of content of laccaic acid A in all products was over 50%. The total contents of laccaic acids A, B and C in lac color food additive products and reagent products were 775-858, 797 and 779 g/kg, respectively. As for the contents of ingredients except pigments in commercial food additive products, the maximum moisture content was about 10%, and ether-soluble substances amounted to 0.5-3.6%.
Hirata
K
K
Tokyo Metropolitan Research Laboratory of Public Health: 3-24-1, Hyakunin-cho, Shinjuku-ku, Tokyo 169-0073, Japan.
Uematsu
Y
Y
Suzuki
K
K
Iida
K
K
Yasuda
K
K
Saito
K
K
D001391:Azo Compounds / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D005505:Food Coloring Agents / Q000737:chemistry*; D010860:Pigments, Biological / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization
D001391:Azo Compounds; D005505:Food Coloring Agents; D010860:Pigments, Biological; C009765:lac dye