The secretion pathway in filamentous fungi: a biotechnological view.
The high capacity of the secretion machinery of filamentous fungi has been widely exploited for the production of homologous and heterologous proteins; however, our knowledge of the fungal secretion pathway is still at an early stage. Most of the knowledge comes from models developed in yeast and higher eukaryotes, which have served as reference for the studies on fungal species. In this review we compile the data accumulated in recent years on the molecular basis of fungal secretion, emphasizing the relevance of these data for the biotechnological use of the fungal cell and indicating how this information has been applied in attempts to create improved production strains. We also present recent emerging approaches that promise to provide answers to fundamental questions on the molecular genetics of the fungal secretory pathway.
Conesa
A
A
Department of Applied Microbiology and Gene Technology, TNO Nutrition and Food Research, Zeist, 3700 AJ, The Netherlands.
Punt
P J
PJ
van Luijk
N
N
van den Hondel
C A
CA
D000818:Animals; D001709:Biotechnology / Q000379:methods*; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism*; D005658:Fungi / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D011994:Recombinant Proteins / Q000378:metabolism
D005656:Fungal Proteins; D011994:Recombinant Proteins