Differential regulation of ftsZ transcription during septation of Streptomyces griseus.
Streptomyces has been known to form two types of septa. The data in this research demonstrated that Streptomyces griseus forms another type of septum near the base of sporogenic hyphae (basal septum). To understand the regulation of the septation machinery in S. griseus, we investigated the expression of the ftsZ gene. S1 nuclease protection assays revealed that four ftsZ transcripts were differentially expressed during morphological differentiation. The vegetative transcript (emanating from P(veg)) is present at a moderate level during vegetative growth, but is switched off within the first 2 h of sporulation. Two sporulation-specific transcripts predominantly accumulated, and the levels increased by approximately fivefold together shortly before sporulation septa begin to form. Consistently, the sporulation-specific transcripts were expressed much earlier and more abundantly in a group of nonsporulating mutants that form their sporulation septa prematurely. Promoter-probe studies with two different reporter systems confirmed the activities of the putative promoters identified from the 5' end point of the transcripts. The levels and expression timing of promoter activities were consistent with the results of nuclease protection assays. The aseptate phenotype of the P(spo) mutant indicated that the increased transcription from P(spo) is required for sporulation septation, but not for vegetative or basal septum formation.
Kwak
J
J
Department of Microbiology, Ohio State University, Columbus, Ohio 43210, USA. kwak91@hotmail.com
Dharmatilake
A J
AJ
Jiang
H
H
Kendrick
K E
KE
D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D003598:Cytoskeletal Proteins; D015964:Gene Expression Regulation, Bacterial; D008854:Microscopy, Electron; D008858:Microscopy, Phase-Contrast; D010641:Phenotype; D011401:Promoter Regions, Genetic; D015719:Single-Strand Specific DNA and RNA Endonucleases / Q000378:metabolism; D013305:Streptomyces griseus / Q000235:genetics*; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D003598:Cytoskeletal Proteins; C071525:FtsZ protein, Bacteria; D015719:Single-Strand Specific DNA and RNA Endonucleases