Cloning of human DECTIN-1, a novel C-type lectin-like receptor gene expressed on dendritic cells.
We identified a new Ca2+-dependent lectin-like receptor gene, DECTIN-1 (HGMW-approved symbol CLECSF12), the human orthologue of mouse Dectin-1, coding for a putative type II transmembrane glycoprotein with an extracellular C-type lectin-like domain. The gene structure and two alternative spliced forms of DECTIN-1 are described. The DECTIN-1 gene was localized in the natural killer gene complex on human Chromosome 12p12.3-p13.2, between OLR1 and CD94 (position 21.8 cM on the genetic map). The DECTIN-1 gene is highly expressed at the mRNA level in dendritic cells and is not further up-regulated during the maturation of these cells with tumor necrosis factor-alpha. The DECTIN-1 gene therefore represents a novel human member of the C-type lectin-like receptor gene family preferentially expressed in dendritic cells.
Hermanz-Falcón
P
P
Unidad de Biología Molecular, Hospital Universitario de la Princesa, Universidad Autónoma de Madrid, Spain.
Arce
I
I
Roda-Navarro
P
P
Fernández-Ruiz
E
E
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D001483:Base Sequence; D002478:Cells, Cultured; D002874:Chromosome Mapping; D002881:Chromosomes, Human, Pair 12 / Q000235:genetics; D003001:Cloning, Molecular; D003713:Dendritic Cells; D020224:Expressed Sequence Tags; D015698:Genomic Library; D006801:Humans; D007694:Killer Cells, Natural; D037102:Lectins; D037181:Lectins, C-Type; D008565:Membrane Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family; D009419:Nerve Tissue Proteins / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution; D000099181:Dectin-1
D037102:Lectins; D037181:Lectins, C-Type; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D000099181:Dectin-1