title: Is exon 5 of the PTEN/MMAC1 gene a prognostic marker in anaplastic glioma?
abstract: Chromosome 10 deletions are among the most common genetic changes in highly malignant glial tumors. It has been noted that loss of heterozygosity (LOH) at 10q23 is a frequent alteration in a variety of human tumors and occurs in approximately 70% of all glioblastomas. By mapping of homozygous deletions on 10q23, a candidate tumor suppressor gene has been isolated, called PTEN for "phosphatase and tensin homolog deleted on chromosome 10" and MMAC1 for "mutated in multiple advanced cancers-1." Mutations of this tumor suppressor gene PTEN/MMAC1 have been reported in anaplastic glial tumors. The objective of this paper was to individuate a prognostic marker in exons 5, 6, 7, and 8 of the PTEN/MMAC1 gene for the high-grade malignant glioma with the most aggressive clinical behavior. In this study, we undertook sequence analysis of these exons in six selected patients with high-grade malignant gliomas who underwent radical aggressive tumor resection followed by radiotherapy within 3 weeks after surgery and subsequent chemotherapy. In them, the exon 5 sequence of the PTEN/MMAC1 gene is suggestive of a genetic survival marker in gliomas with high-grade malignancy.
authors.affiliation: r.alexx@tiscalinet.it
authors.forename: A
authors.initials: A
authors.lastname: Rustia
authors.forename: V
authors.initials: V
authors.lastname: Wierzbicki
authors.forename: L
authors.initials: L
authors.lastname: Marrocco
authors.forename: A
authors.initials: A
authors.lastname: Tossini
authors.forename: C
authors.initials: C
authors.lastname: Zamponi
authors.forename: F
authors.initials: F
authors.lastname: Lista
mesh_terms: D000328:Adult; D000368:Aged; D000708:Anaplasia / Q000235:genetics / Q000401:mortality / Q000473:pathology; D001932:Brain Neoplasms / Q000235:genetics* / Q000401:mortality* / Q000473:pathology; D005091:Exons / Q000235:genetics*; D005260:Female; D005819:Genetic Markers / Q000235:genetics; D005910:Glioma / Q000235:genetics* / Q000401:mortality* / Q000473:pathology; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation / Q000235:genetics; D051059:PTEN Phosphohydrolase; D010744:Phosphoric Monoester Hydrolases / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011379:Prognosis; D017422:Sequence Analysis, DNA; D015996:Survival Rate; D025521:Tumor Suppressor Proteins / Q000235:genetics*
chemical_list: D005819:Genetic Markers; D025521:Tumor Suppressor Proteins; D010744:Phosphoric Monoester Hydrolases; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human