[Generation of monoclonal antibody Fab fragments to parathyroid hormone related protein by phage display technology].
OBJECTIVE
To develop monoclonal antibody Fab fragments using display technique.


METHODS
The mouse IgG Fab genes of heavy and light chains were amplified from spleen cells of a parathyroid hormone-related protein (PTHrP) immunized mouse. The combinatorial phage antibody library was prepared by inserting both heavy and light chain Fab genes into phagemid vector pComb3 and followed by infection of helper phage. The library was selected by purified recombinant PTHrP.


RESULTS
The combinatorial phage antibody library was constructed successfully and the specific mouse Fabs to PThrP were selected and expressed in E. coli.


CONCLUSIONS
The selected specific mouse Fabs can recognize PTHrP with high specificity.
Dou
L
L
Institute of Virology, Chinese Academy of Preventive Medicine, Beining 100052, China.
Liang
M
M
An
N
N
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000235:genetics; D001435:Bacteriophages / Q000235:genetics*; D004926:Escherichia coli / Q000378:metabolism; D015723:Gene Library; D007140:Immunoglobulin Fab Fragments / Q000096:biosynthesis* / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D044162:Parathyroid Hormone-Related Protein; D036361:Peptide Hormones / Q000276:immunology*
D000911:Antibodies, Monoclonal; D007140:Immunoglobulin Fab Fragments; D044162:Parathyroid Hormone-Related Protein; D036361:Peptide Hormones