Human colon cancer produces a factor which induces the proliferation of venous endothelial cells.
This study was performed to investigate whether colorectal cancers produce proliferative factors for venous endothelial cells, and whether the proliferative activity is related to basic FGF and VEGF and also to the clinicopathological findings. Surgically resected specimens of 17 colorectal cancer patients were fragmented and cultured, and the supernatant was collected. A human umbilical endothelial cell line (EA-hy 926 cells) was incubated with the supernatant. The proliferative activity was examined and the levels of basic FGF and VEGF were measured. The activities were found to be significantly related to VEGF, the depth of tumor invasion and the tumor stage.
Iwasaki
K
K
Department of Surgery II, Nagasaki University School of Medicine, Nagasaki 852-8102, Japan. kazuomi@bronze.ocn.ne.jp
Yamamoto
M
M
Minami
S
S
Komuta
K
K
Yamaguchi
J
J
Furui
J
J
Kanematsu
T
T
D000328:Adult; D000368:Aged; D002455:Cell Division / Q000187:drug effects*; D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000473:pathology; D003470:Culture Media; D016228:Endothelial Growth Factors / Q000096:biosynthesis / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000166:cytology* / Q000187:drug effects; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000096:biosynthesis / Q000494:pharmacology; D006801:Humans; D008222:Lymphokines / Q000096:biosynthesis / Q000494:pharmacology*; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D014407:Tumor Cells, Cultured; D014471:Umbilical Veins; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
D003470:Culture Media; D016228:Endothelial Growth Factors; D008222:Lymphokines; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; D016222:Fibroblast Growth Factor 2