title: Cytotoxicity of halothane on human gingival fibroblast cultures in vitro.
abstract: Recently halothane has been reported to be the most suitable alternative to chloroform in dissolving gutta-percha. Periapical tissue toxicity of halothane is not completely known. In this study gutta-percha dissolved by halothane was evaluated with the almar blue dye assay using human gingival fibroblast cultures. The cytotoxic effects of halothane on human gingival fibroblasts depended on the exposure dose, frequency, and duration. A reduced concentration and smaller amount of gutta-percha solvents may minimize the cytotoxic effects on host tissues.
authors.affiliation: Department of Dentistry, Chung Shan Medical and Dental College Hospital, Taichung, Taiwan, Republic of China.
authors.forename: Y C
authors.initials: YC
authors.lastname: Chang
authors.forename: M Y
authors.initials: MY
authors.lastname: Chou
mesh_terms: D002448:Cell Adhesion / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D048429:Cell Size / Q000187:drug effects; D002478:Cells, Cultured; D003239:Connective Tissue Cells / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects*; D005881:Gingiva / Q000166:cytology / Q000187:drug effects*; D006171:Gutta-Percha / Q000737:chemistry; D006221:Halothane / Q000008:administration & dosage / Q000737:chemistry / Q000633:toxicity*; D006801:Humans; D007202:Indicators and Reagents; D010486:Periapical Tissue / Q000166:cytology / Q000187:drug effects; D012387:Root Canal Filling Materials / Q000737:chemistry; D012995:Solubility; D012997:Solvents / Q000008:administration & dosage / Q000737:chemistry / Q000633:toxicity*; D013778:Tetrazolium Salts; D013997:Time Factors
chemical_list: D007202:Indicators and Reagents; D012387:Root Canal Filling Materials; D012997:Solvents; D013778:Tetrazolium Salts; C042207:4-anisyltetrazolium blue; D006171:Gutta-Percha; D006221:Halothane