Reactive blue 2 induces calcium oscillations in HeLa cells.
Reactive Blue 2 (RB), which is used as an ATP receptor antagonist, induced Ca(2+) oscillations in HeLa cells. RB-induced Ca(2+) oscillations were abolished in Ca(2+)-free solution. RB, however, did not affect Ca(2+) influx measured by Mn(2+) quenching. The PLC cascade and intracellular Ca(2+) release were involved as U73122 and thapsigargin inhibited RB-induced Ca(2+) oscillations. RB enhanced a Ca(2+) response to histamine that is linked to the PLC cascade. RB may activate the PLC cascade in an extracellular Ca(2+)-dependent manner and induce Ca(2+) oscillations.
Okuda
A
A
Department of Applied Biology, Kyoto Institute of Technology, Matsugasaki, Kyoto, 606-8585 Japan. okuda@bpe.es.osaka-u.ac.jp
Furuya
K
K
Kiyohara
T
T
D015220:Calcium Channels / Q000502:physiology*; D020013:Calcium Signaling / Q000502:physiology*; D018929:Cell Culture Techniques; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D006367:HeLa Cells; D006801:Humans; D014227:Triazines / Q000494:pharmacology*
D015220:Calcium Channels; D004791:Enzyme Inhibitors; D014227:Triazines; C012842:Cibacron Blue F 3GA