title: Are standard human coagulation tests suitable in pigs and calves during extracorporeal circulation?
abstract: The thrombogenicity of membrane oxygenators as well as clotting parameters profiles, using standard human clotting tests, was analyzed in calves and pigs during 6 h perfusion. Three calves and 3 pigs were connected to extracorporeal circulation with standard heparinization. Blood samples were taken for coagulation variables throughout perfusion, and oxygenators were examined for clot deposits at the end of the experiment. Two out of 3 oxygenators of the calf group presented clot deposits while none in the pig group did. Baseline coagulation variables of pigs showed values similar to those of humans while neither extrinsic nor intrinsic pathways could be activated in calves with standard human coagulation tests. The calf model, in conclusion, was confirmed to be a difficult model for the testing of extracorporeal circulation device resistance to thrombus formation, which is, however, not reflected by standard human coagulation tests. The pig model is a better model in which both coagulation pathways could be activated with standard human coagulation tests.
authors.affiliation: Clinic for Cardiovascular Surgery, Centre Hospitalier Universitaire Vaudois, CH-1011 Lausanne, Switzerland. xavier.mueller@chuv.hospvd.ch
authors.forename: X M
authors.initials: XM
authors.lastname: Mueller
authors.forename: H T
authors.initials: HT
authors.lastname: Tevaearai
authors.forename: D
authors.initials: D
authors.lastname: Jegger
authors.forename: O
authors.initials: O
authors.lastname: Tucker
authors.forename: L K
authors.initials: LK
authors.lastname: von Segesser
mesh_terms: D000328:Adult; D000704:Analysis of Variance; D000818:Animals; D001780:Blood Coagulation Tests; D002315:Cardiopulmonary Bypass / Q000379:methods; D002417:Cattle; D004195:Disease Models, Animal; D005112:Extracorporeal Circulation / Q000379:methods*; D006439:Hemodynamics / Q000502:physiology; D006493:Heparin / Q000494:pharmacology; D006801:Humans; D008991:Monitoring, Physiologic / Q000379:methods; D011336:Probability; D012680:Sensitivity and Specificity; D013045:Species Specificity; D013552:Swine; D013923:Thromboembolism / Q000517:prevention & control
chemical_list: D006493:Heparin