Application of microassays for investigation of cellulase abrasive activity and backstaining.
Model microassays were used for testing the denim-washing performance and indigo backstaining for Trichoderma reesei and Chrysosporium lucknowense commercial cellulase preparations on a 'test-tube scale'. C. lucknowense preparation demonstrated a higher potential in the denim biostoning process. The performance of four purified cellulases (two endoglucanases and two cellobiohydrolases) from C. lucknowense on cotton textiles was assayed, and the key enzyme (endoglucanase with a molecular weight of 25 kDa) responsible for high abrasion effects on denim fabrics was found.
Sinitsyn
A P
AP
Department of Chemistry, M. V. Lomonosov Moscow State University, 119899, Moscow, Russia. apsinitsyn@enzyme.chem.msu.ru
Gusakov
A V
AV
Grishutin
S G
SG
Sinitsyna
O A
OA
Ankudimova
N V
NV
D002480:Cellulase / Q000737:chemistry* / Q000302:isolation & purification; D005658:Fungi / Q000201:enzymology; D003368:Gossypium / Q000737:chemistry; D014242:Trichoderma / Q000201:enzymology
D002480:Cellulase