Radical degradation of high molecular weight hyaluronan: inhibition of the reaction by ibuprofen enantiomers.
The antioxidative and/or free-radical-scavenging activities of R-(-)- and S-(+)-ibuprofen enantiomers, as well as of the drug racemate, were studied in vitro on measuring the kinetics of (uninhibited or drug-inhibited) degradation of high molecular weight hyaluronan by hydroxyl radicals. The continual flux of OH radicals at aerobic conditions was maintained by the H2O2 + Cu2+ system. The kinetics of hyaluronan degradation was monitored indirectly by capillary viscometry. Under experimental conditions, with no drug addition, the relative viscosity ([eta]rel) decreased continuously, reaching 13% of the initial [eta]rel. value in 4 h. Each drug tested exhibited a dose-dependent protective effect against hyaluronan degradation, however R-(-)-ibuprofen demonstrated a slightly greater activity than the drug S-(+)-enantiomer.
Soltés
L
L
Institute of Experimental Pharmacology, Slovak Academy of Sciences, Bratislava, Slovak Republic. exfasolt@savba.sk
Lath
D
D
Mendichi
R
R
Bystrický
P
P
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry*; D004347:Drug Interactions; D006820:Hyaluronic Acid / Q000737:chemistry*; D007052:Ibuprofen / Q000737:chemistry*; D008970:Molecular Weight; D013237:Stereoisomerism; D014783:Viscosity
D000894:Anti-Inflammatory Agents, Non-Steroidal; D006820:Hyaluronic Acid; D007052:Ibuprofen