title: BIACORE J: a new platform for routine biomolecular interaction analysis.
abstract: SPR biosensor technology continues to evolve. The recently released platform from Biacore AB (Uppsala, Sweden), BIACORE J, is designed for the routine analysis of biomolecular interactions. Using an antibody-protein A and a ligand-receptor system, we demonstrate the utility of BIACORE J in determining active concentration and binding affinities. The results from these studies illustrate the high sensitivity of the instrument and its ability to generate reproducible binding responses. The BIACORE J is easy to operate and useful in diverse applications, making SPR technology widely accessible as a research tool.
authors.affiliation: Center for Biomolecular Interaction Analysis, School of Medicine, University of Utah, Salt Lake City, UT 84132, USA.
authors.forename: R L
authors.initials: RL
authors.lastname: Rich
authors.forename: D G
authors.initials: DG
authors.lastname: Myszka
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D000937:Antigen-Antibody Reactions; D001666:Binding Sites, Antibody; D015374:Biosensing Techniques; D004305:Dose-Response Relationship, Drug; D004306:Dose-Response Relationship, Immunologic; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D008024:Ligands; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism*; D012015:Reference Standards; D015203:Reproducibility of Results; D013205:Staphylococcal Protein A / Q000378:metabolism*; D020349:Surface Plasmon Resonance / Q000295:instrumentation* / Q000379:methods*; D047990:TNF Receptor-Associated Factor 1; D047992:TNF Receptor-Associated Factor 2; D013997:Time Factors
chemical_list: D000911:Antibodies, Monoclonal; D008024:Ligands; D011506:Proteins; D013205:Staphylococcal Protein A; D047990:TNF Receptor-Associated Factor 1; D047992:TNF Receptor-Associated Factor 2