[Effect of Fusheng powder on activity of vascular endothelial cells and its adhesion to polymorphonuclear neutrophils].
OBJECTIVE
To explore the effect of Fusheng powder on the adhesion of polymorphonuclear neutrophils (PMN) in human peripheral blood to vascular endothelial cells (VEC).


METHODS
Using cultured human umbilical vein cells as target cells, while being incubated with high glucose (HG, 30 mmol/L), high-lipid (HL, 20%), tumor necrosis factor-alpha (TNF-alpha, 10%) and hypoxia (95% N2, 5% CO2, 37 degrees C, 30 min, and then in the air 30 min) for 24 h, respectively, the activity of VEC and the adhesive effect of PMN to VEC were surveyed.


RESULTS
It revealed that HG, HL, TNF-alpha and hypoxia could enhance obviously the adhesion of PMN to VEC, the cell attachment rate in normal control, HG group, TNF-alpha, hypoxia and HL after 30 minutes contact were 100%, 129.6%, 136.7%, 151.4% and 167.7% (all P < 0.01, compared with control group) respectively, but high and low dosage of the Fusheng powder could significantly inhibit the adhesion of PMN to VEC.


CONCLUSIONS
HG, HL, TNF-alpha and hypoxia could all activate EC respectively and enhance the adhesion of PMN to VEC, but the Fusheng powder could protect VEC and partly block the effect of this adhesion. However, the effects of Fusheng powder on anti-adhesion are of great importance in clinical cardiovascular and cerebral vascular diseases.
Lei
Y
Y
Department of Pathophysiology, Faculty of Basic Medicine, Beijing University of Traditional Chinese Medicine and Pharmacology, Beijing 100029.
Hao
Y
Y
Qiu
Q
Q
D000328:Adult; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000166:cytology*; D006801:Humans; D007231:Infant, Newborn; D009504:Neutrophils / Q000166:cytology; D051381:Rats; D017208:Rats, Wistar; D014471:Umbilical Veins / Q000166:cytology
D004365:Drugs, Chinese Herbal