Neuroprotective efficacy of AR-A008055, a clomethiazole analogue, in a global model of acute ischaemic stroke and its effect on ischaemia-induced glutamate and GABA efflux in vitro.
We have investigated the neuroprotective properties of AR-A008055 [(+/-)-1-(4-methyl-5-thiazolyl-1-phenyl-methylamine], a novel compound structurally related to clomethiazole. Administration (i.p.) of (+/-)-AR-A008055 60 min after 5 min of global cerebral ischaemia in gerbils produced a dose-dependent protection of the hippocampus from damage. Both enantiomers [(R)-(+)-AR-A008055 and (S)-(-)- AR-A008055] at 600 micromol/kg produced similar protection to that following clomethiazole (600& micromol/kg) and both produced similar and sustained neuroprotection, at 4, 7 and 21 days post-insult. When infused intravenously over a 2-h period, both enantiomers produced concentration-dependent neuroprotection, with the enantiomers providing similar protection at every plasma concentration (50-200 nmol/ml). The efficacy of (S)-(-)-AR-A008055 was similar to clomethiazole, but it was slightly less potent. Ischaemia-induced glutamate efflux from rat brain cortical prisms in vitro was inhibited by both isomers (100 microM). The inhibitory effect of (R)-(+)-AR-A008055 was blocked by bicuculline (10 microM) and picrotoxin (100 microM), while the effect of (S)-(-)-AR-A008055 was only antagonised by picrotoxin. This indicated that (S)-(-)-AR-A008055, like clomethiazole, is able to open the GABA(A)-chloride channel in the absence of endogenous GABA. (R)-(+)-AR-A008055 was more potent than (S)-(-)-AR-A008055 in enhancing the concentration of GABA in the medium following 30 min exposure of tissue to the ischaemic conditions, suggesting that it is an effective GABA uptake inhibitor. This action may explain both its effect on glutamate efflux in vitro and its neuroprotective effect in vivo.
Nelson
R M
RM
Department of Pharmacology, School of Pharmacy, De Montfort University, The Gateway, Leicester LE1 9BH, UK.
Hainsworth
A H
AH
Lambert
D G
DG
Jones
J A
JA
Murray
T K
TK
Richards
D A
DA
Gabrielsson
J
J
Cross
A J
AJ
Green
A R
AR
D000818:Animals; D002545:Brain Ischemia / Q000378:metabolism*; D002719:Chlormethiazole / Q000031:analogs & derivatives* / Q000494:pharmacology*; D005260:Female; D005849:Gerbillinae; D018698:Glutamic Acid / Q000378:metabolism*; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008744:Methylamines / Q000494:pharmacology*; D018696:Neuroprotective Agents / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D020521:Stroke / Q000378:metabolism; D013844:Thiazoles / Q000494:pharmacology*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
C436486:1-(4-methyl-5-thiazolyl)-1-phenylmethylamine; D008744:Methylamines; D018696:Neuroprotective Agents; D013844:Thiazoles; D002719:Chlormethiazole; D018698:Glutamic Acid; D005680:gamma-Aminobutyric Acid