title: Determination of the extracellular concentration of glycine in the rat spinal cord dorsal horn by quantitative microdialysis.
abstract: Two quantitative microdialysis methods were used to determine the extracellular concentration of glycine in the dorsal spinal cord of halothane-anaesthetised rats. Extracellular glycine determined by zero net flux was 2.6+/-0.3 microM and by the zero flow method was 3.3+/-0.3 microM. For comparison the glycine content of cerebrospinal fluid was determined to be 6.4+/-1.1 microM. There was no correlation between the extracellular and the cerebrospinal fluid concentrations.
authors.affiliation: Department of Pharmacology, Division of Neuroscience, The Medical School, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK. k.j.whitehead@bham.ac.uk
authors.forename: K J
authors.initials: KJ
authors.lastname: Whitehead
authors.forename: J P
authors.initials: JP
authors.lastname: Manning
authors.forename: C G
authors.initials: CG
authors.lastname: Smith
authors.forename: N G
authors.initials: NG
authors.lastname: Bowery
mesh_terms: D000818:Animals; D005110:Extracellular Space / Q000378:metabolism*; D005998:Glycine / Q000134:cerebrospinal fluid*; D008297:Male; D017551:Microdialysis / Q000379:methods*; D020671:Posterior Horn Cells / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism*; D009435:Synaptic Transmission / Q000502:physiology*
chemical_list: D016194:Receptors, N-Methyl-D-Aspartate; D005998:Glycine