Expression of interleukin-1 beta in rat dorsal root ganglia.
The expression of interleukin-1beta was examined in dorsal root ganglion (DRG) neurons from adult rats using non-radioactive in situ hybridization and immunocytochemistry. At all spinal levels, approximately 70% of the DRG neurons appeared to express IL-1beta mRNA; about 80% of these DRG neurons actually appeared to produce the IL-1beta protein at markedly varying levels. The expression of IL-1beta was found in large as well as in intermediate diameter sensory neurons but only sporadically in the population of small sensory neurons. The population of IL-1beta immunopositive sensory neurons included most of the large calretinin-positive Ia afferents, but only a few of the small substance P/CGRP positive sensory neurons. In situ hybridization staining for the detection of type 1 IL-1 receptor showed expression of this receptor by most of the sensory neurons as well as by supportive glial-like cells, presumably satellite cells. The functional significance of IL-1beta in the DRG neurons needs to be elucidated, but we speculate that IL-1beta produced by DRG neurons may be an auto/paracrine signalling molecule in sensory transmission.
Copray
J C
JC
Department of Medical Physiology, University of Groningen, A. Deusinglaan 1, Bloemsingel 10, 9713 AV KZ, Groningen, Netherlands. j.c.v.m.copray@med.rug.nl
Mantingh
I
I
Brouwer
N
N
Biber
K
K
Küst
B M
BM
Liem
R S
RS
Huitinga
I
I
Tilders
F J
FJ
Van Dam
A M
AM
Boddeke
H W
HW
D000818:Animals; D064032:Calbindin 2; D015740:Calcitonin Gene-Related Peptide / Q000096:biosynthesis; D048429:Cell Size; D003593:Cytoplasm / Q000378:metabolism / Q000648:ultrastructure; D005260:Female; D005727:Ganglia, Spinal / Q000166:cytology / Q000378:metabolism*; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007375:Interleukin-1 / Q000096:biosynthesis* / Q000235:genetics; D008297:Male; D016253:Microscopy, Immunoelectron; D009457:Neuroglia / Q000166:cytology / Q000378:metabolism; D009475:Neurons, Afferent / Q000166:cytology / Q000378:metabolism*; D011132:Polyribosomes / Q000378:metabolism / Q000648:ultrastructure; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017472:Receptors, Interleukin-1 / Q000096:biosynthesis; D053573:Receptors, Interleukin-1 Type I; D064030:S100 Calcium Binding Protein G / Q000096:biosynthesis; D013373:Substance P / Q000096:biosynthesis
C576512:Calb2 protein, rat; D064032:Calbindin 2; D007375:Interleukin-1; D012333:RNA, Messenger; D017472:Receptors, Interleukin-1; D053573:Receptors, Interleukin-1 Type I; D064030:S100 Calcium Binding Protein G; D013373:Substance P; D015740:Calcitonin Gene-Related Peptide