title: Simultaneous determination of F-beta-alanine and beta-alanine in plasma and urine with dual-column reversed-phase high-performance liquid chromatography.
abstract: F-beta-Alanine and beta-alanine were detected in plasma and urine samples with fluorescence detection of orthophthaldialdehyde derivatives of F-beta-alanine and beta-alanine after separation with dual-column reversed-phase HPLC. The detection limits of F-beta-alanine and beta-alanine in the HPLC system were approximately 0.3 and 0.7 pmol, respectively. The procedure proved to be very reproducible with intra-assay RSDs and inter-assay RSDs being less than 8%. The usefulness of the method was demonstrated by the analysis of the F-beta-alanine and beta-alanine concentrations in plasma and urine samples from tumor patients treated with S-1 (Tegafur, 5-chloro-2,4-dihydroxypyridine and potassium oxonate in a molar ratio of 1:0.4:1).
authors.affiliation: Academic Medical Center, University of Amsterdam, Emma Children's Hospital and Department of Clinical Chemistry, The Netherlands. a.b.vankuilenberg@amc.uva.nl
authors.forename: A B
authors.initials: AB
authors.lastname: Van Kuilenburg
authors.forename: A E
authors.initials: AE
authors.lastname: Stroomer
authors.forename: G J
authors.initials: GJ
authors.lastname: Peters
authors.forename: A H
authors.initials: AH
authors.lastname: Van Gennip
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D009369:Neoplasms / Q000097:blood / Q000188:drug therapy / Q000652:urine; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence; D005641:Tegafur / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000627:therapeutic use; D015091:beta-Alanine / Q000032:analysis* / Q000097:blood / Q000652:urine
chemical_list: D015091:beta-Alanine; D005641:Tegafur