title: Frequent demonstration of human herpesvirus 8 (HHV-8) in bone marrow biopsy samples from Turkish patients with multiple myeloma (MM).
abstract: In order to investigate the frequency of HHV-8 in MM patients from another geographic location, we obtained fresh bone marrow (BM) biopsies from Turkish patients with MM (n = 21), monoclonal gammopathy of undetermined significance (MGUS) (n = 2), plasmacytoma (n = 1) with BM plasma cell infiltration, various hematological disorders (n = 6), and five healthy Turkish controls. The frequency of HHV-8 was analyzed by polymerase chain reaction (PCR) in two independent laboratories in the USA and in Turkey. Using fresh BM biopsies, 17/21 MM patients were positive for HHV-8 whereas all five healthy controls, and six patients with other hematological disorders were negative. Two patients with MGUS, and one patient with a solitary plasmacytoma were also negative. The data from the two laboratories were completely concordant. Also using primer pairs for v IRF and v IL-8R confirmed the results observed with the KS330233 primers. Furthermore, sequence analysis demonstrated a C3 strain pattern in the ORF26 region which was also found in MM patients from the US. Thus, HHV-8 is present in the majority of Turkish MM patients, and the absence of the virus in healthy controls further supports its role in the pathogenesis of MM.
authors.affiliation: Ibni Sina Hospital, Department of Hematology, Ankara University School of Medicine, Sihhiye, Turkey.
authors.forename: M
authors.initials: M
authors.lastname: Beksac
authors.forename: M
authors.initials: M
authors.lastname: Ma
authors.forename: C
authors.initials: C
authors.lastname: Akyerli
authors.forename: M
authors.initials: M
authors.lastname: DerDanielian
authors.forename: L
authors.initials: L
authors.lastname: Zhang
authors.forename: J
authors.initials: J
authors.lastname: Liu
authors.forename: M
authors.initials: M
authors.lastname: Arat
authors.forename: N
authors.initials: N
authors.lastname: Konuk
authors.forename: H
authors.initials: H
authors.lastname: Koc
authors.forename: T
authors.initials: T
authors.lastname: Ozcelik
authors.forename: R
authors.initials: R
authors.lastname: Vescio
authors.forename: J R
authors.initials: JR
authors.lastname: Berenson
mesh_terms: D001706:Biopsy; D001853:Bone Marrow / Q000473:pathology* / Q000821:virology*; D006566:Herpesviridae Infections / Q000453:epidemiology / Q000821:virology*; D019288:Herpesvirus 8, Human / Q000302:isolation & purification*; D006801:Humans; D009101:Multiple Myeloma / Q000453:epidemiology / Q000209:etiology / Q000473:pathology / Q000821:virology*; D014421:Turkey / Q000453:epidemiology