Involvement of nuclear factor-kB in the expression of opiate withdrawal.
1. To investigate the role of NF-kB in the expression of opiate withdrawal, the effects of PDTC, an inhibitor of NF-kB activation, was studied on acute opiate withdrawal induced by morphine in vitro. 2. After a 4 min in vitro exposure to morphine, a strong contracture of guinea pig isolated ileum was observed after the addition of naloxone. 3. PDTC (1x10(-8)-5x10(-8)-1x10(-7) M) was able to reduce the naloxone-induced contracture after exposure to the opioid agonist in a concentration-dependent fashion. 4. The results of the present study indicate that NF-kB is involved in the expression of opiate withdrawal thus extending and explaining previous papers performed with dexamethasone and selective arachidonic acid metabolites inhibitors.
Capasso
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Department of Pharmaceutical Sciences, University of Salerno, Italy. annacap@unisa.it
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D006168:Guinea Pigs; D007082:Ileum / Q000187:drug effects / Q000378:metabolism; D008297:Male; D009020:Morphine / Q000009:adverse effects; D016328:NF-kappa B / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000502:physiology*; D009294:Narcotics / Q000009:adverse effects; D009293:Opioid-Related Disorders / Q000378:metabolism*; D011759:Pyrrolidines / Q000494:pharmacology; D013375:Substance Withdrawal Syndrome / Q000378:metabolism*; D013859:Thiocarbamates / Q000494:pharmacology
D000975:Antioxidants; D016328:NF-kappa B; D009294:Narcotics; D011759:Pyrrolidines; D013859:Thiocarbamates; C020972:pyrrolidine dithiocarbamic acid; D009020:Morphine