[Cloning and sequencing of complete TT virus genome from Beijing isolate of China].
OBJECTIVE
To obtain the complete TT virus genome from a Beijing isolate of China.


METHODS
PCR was performed for amplifying the TT virus genome from serum of a patient with non A-G hepatitis. Five overlapped fragments, that are 199 bp (1-199), 1 267 bp (90-1356), 878 bp (1354-2231),1129 bp(2178-3306),'434 bp(3306-3739), respectively, were obtained. These PCR products were sequenced by standard method.


RESULTS
The complete TT virus genome of Beijing isolate consists of 3 739 nucleotides involving two ORFs. The first ORF locates at 589-2902 nt, encoding 770 amino acids, the second ORF locates at 107-715 nt, encoding 202 amino acids. 5' and 3' terminus of the genome are non-coding regions including 106 bp and 837 bp respectively. In this genome, A is 31.2%, C 25.4%, G 22.5% and T 20.9%. The homology of Beijing isolate and Japanese TT virus was more than 95% at both nucleotide and amino acid levels.


CONCLUSIONS
We successfully cloned and sequenced the complete TT virus genome from a Chinese patient. This Beijing TT virus isolate and that reported by Japan all belong to a same genotype.
He
Z
Z
Research Center of Virology, Beijing Ditan Hospital, Beijing 100011, China.
Zhou
Y
Y
Zhang
Q
Q
D000595:Amino Acid Sequence; D001483:Base Sequence; D002681:China; D003001:Cloning, Molecular; D005814:Genes, Viral / Q000235:genetics*; D006525:Hepatitis, Viral, Human / Q000821:virology*; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D017421:Sequence Analysis; D017385:Sequence Homology; D022783:Torque teno virus / Q000235:genetics* / Q000302:isolation & purification