title: Purification and characterization of a vulnificolysin-like cytolysin produced by Vibrio tubiashii.
abstract: An extracellular cytolysin from Vibrio tubiashii was purified by sequential hydrophobic interaction chromatography with phenyl-Sepharose CL-4B and gel filtration with Sephacryl S-200. This protein is sensitive to heat and proteases, is inhibited by cholesterol, and has a molecular weight of 59,000 and an isoelectric point of 5.3. In addition to lysing various erythrocytes, it is cytolytic and/or cytotoxic to Chinese hamster ovary cells, Caco-2 cells, and Atlantic menhaden liver cells in tissue culture. Lysis of erythrocytes occurs by a multihit process that is dependent on temperature and pH. Twelve of the first 17 N-terminal amino acid residues (Asp-Asp-Tyr-Val-Pro-Val-Val-Glu-Lys-Val-Tyr-Tyr-Ile-Thr-Ser-Ser-Lys) are identical to those of the Vibrio vulnificus cytolysin.
authors.affiliation: Divisions of Virulence Assessment, Center for Food Safety and Applied Nutrition, U.S. Food and Drug Administration, Washington, DC 20204, USA. mkothary@cfsan.fda.gov
authors.forename: M H
authors.initials: MH
authors.lastname: Kothary
authors.forename: R B
authors.initials: RB
authors.lastname: Delston
authors.forename: S K
authors.initials: SK
authors.lastname: Curtis
authors.forename: B A
authors.initials: BA
authors.lastname: McCardell
authors.forename: B D
authors.initials: BD
authors.lastname: Tall
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D016466:CHO Cells / Q000187:drug effects; D018938:Caco-2 Cells / Q000187:drug effects; D002478:Cells, Cultured / Q000187:drug effects; D006224:Cricetinae; D003603:Cytotoxins / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification* / Q000633:toxicity*; D004912:Erythrocytes / Q000187:drug effects; D005399:Fishes; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D008969:Molecular Sequence Data; D014733:Vibrio / Q000378:metabolism*
chemical_list: D003603:Cytotoxins