[Effect of recombinant human interleukin-1 beta on K+ channels of mice bone marrow stromal cells].
In the present study, both cell-attached and inside-out mode of patch clamp technique were applied to detect the effect of recombinant human interleukin-1 beta (IL-1 beta) on K+ channels of mice bone marrow stromal cells. A 16.7 pS voltage-dependent K+ channel resembling the delayed rectifier K+ channel in excitable cells was identified and characterized. Under stimulation of IL-1 beta (1000 U/ml), the single channel conductance increased to 26.1 +/- 3.6 pS (P < 0.01). IL-1 beta also markedly increased the open time constant and the open probability, but decreased the closed time constant as compared to the same patches without IL-1 beta. In addition, IL-1 beta induced multi-channel activities in about 80 percent patches by inducing more K+ channels of the same type open. These results suggest that activation of K+ channels plays a role in signal transduction of interleukin-1.
Chen
N N
NN
Institute of Pharmaceutical Sciences, First Military Medical University, Guangzhou 510515.
Wu
S G
SG
D000818:Animals; D001854:Bone Marrow Cells / Q000378:metabolism*; D005260:Female; D007375:Interleukin-1 / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D018408:Patch-Clamp Techniques; D015221:Potassium Channels / Q000187:drug effects* / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology; D015398:Signal Transduction; D017154:Stromal Cells / Q000378:metabolism
D007375:Interleukin-1; D015221:Potassium Channels; D011994:Recombinant Proteins