Features of the antibody response attributable to plasmid backbone adjuvanticity after DNA immunization.
DNA vaccination induces antigen-specific immune responses with characteristics distinct from other vaccination modes. In the present study, the contribution of the plasmid backbone adjuvant effect to the quality of the DNA-raised antibody response was investigated. For this purpose, three intradermal primings were compared in mice using: (1) the recombinant Schistosoma haematobium glutathione S-transferase antigen (rSh28GST): (2) rSh28GST supplemented with a non-coding plasmid; and (3) a Sh28GST-encoding plasmid. In contrast to immunization with the protein, DNA immunization elicited a very stable antibody (Ab) response over a prolonged period of time. This feature was attributed to the plasmid backbone, because co-administration of the non-coding plasmid with rSh28GST allowed the maintenance of the specific Ab response. A strong anamnestic Ab response was induced after intradermal boost with rSh28GST only in the mice primed with pMSh. This indicated that the selective ability of DNA vaccination to induce memory humoral response was independent of the plasmid backbone. In contrast the plasmid backbone was found to strongly participate in the preferential IgG2a Ab production observed. These results suggest that, following DNA immunization, the Th1-biased profile and the maintenance of the long-lived Ab response could be attributed to an adjuvant effect of the plasmid backbone during priming, whereas the strength of B-cell memory was independent of this effect.
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Relations Hôte-Parasite et Stratégies Vaccinales, INSERM U 167, Institut Pasteur de Lille, 1 Rue du professeur Calmette, BP-245, F-59019, Cedex, Lille, France. maxime.herve@pasteur-lille.fr
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D000276:Adjuvants, Immunologic / Q000008:administration & dosage* / Q000235:genetics*; D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis*; D002460:Cell Line; D005260:Female; D005982:Glutathione Transferase / Q000235:genetics / Q000276:immunology; D007117:Immunization, Secondary; D007156:Immunologic Memory / Q000235:genetics; D007271:Injections, Intradermal; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids / Q000008:administration & dosage* / Q000276:immunology*; D012548:Schistosoma haematobium / Q000201:enzymology / Q000235:genetics / Q000276:immunology*; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000276:immunology*
D000276:Adjuvants, Immunologic; D000909:Antibodies, Helminth; D019444:Vaccines, DNA; D005982:Glutathione Transferase