title: Maternal-fetal transfer of saquinavir studied in the ex vivo placental perfusion model.
abstract: OBJECTIVE
  This study was undertaken to determine the placental transfer of the human immunodeficiency virus protease inhibitor saquinavir.
  
  
  METHODS
  An ex vivo perfused human placental cotyledon model was used. Ten placental perfusion studies were performed, with concentrations of saquinavir in the maternal compartment ranging from 322 to 2197 ng/mL (within reference therapeutic ranges). Drug concentrations were determined by high-performance liquid chromatography.
  
  
  RESULTS
  The mean (+/- SD) fetal transfer rate of saquinavir was 1.8% +/- 1.6%, and the mean (+/- SD) clearance index was 0.05 +/- 0.05. A mean (+/- SD) of 1.6% +/- 3.1% of the perfused saquinavir was retained by the cotyledon. The small amount of saquinavir that crossed the placenta corresponded to the fraction not bound to human serum albumin.
  
  
  CONCLUSIONS
  The low rate of placental transfer of saquinavir suggests that use of this antiretroviral drug by pregnant women may not lead to significant exposure of the fetus.
authors.affiliation: Université Paris Sud, Paris, France.
authors.forename: F
authors.initials: F
authors.lastname: Forestier
authors.forename: P
authors.initials: P
authors.lastname: de Renty
authors.forename: G
authors.initials: G
authors.lastname: Peytavin
authors.forename: E
authors.initials: E
authors.lastname: Dohin
authors.forename: R
authors.initials: R
authors.lastname: Farinotti
authors.forename: L
authors.initials: L
authors.lastname: Mandelbrot
mesh_terms: D005260:Female; D017320:HIV Protease Inhibitors / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D008431:Maternal-Fetal Exchange; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D019258:Saquinavir / Q000378:metabolism*
chemical_list: D017320:HIV Protease Inhibitors; D019258:Saquinavir