title: Effect of procainamide on the postrepolarization refractoriness in cardiac muscle: evaluation using the block coupling interval in the artificial isthmus model in the canine right atrium.
abstract: The post-repolarization refractoriness (PRR) is an important factor to determine the conduction block in cardiac muscle. Recently, we proposed the block coupling interval (BCI) as an useful electrophysiological index for evaluating the PRR. In the present study, the effect of procainamide on PRR was evaluated using the BCI and the effective refractory period (ERP). In five beagle dogs, radiofrequency linear ablation was performed on the right atrial surface parallel to the AV groove, forming an artificial isthmus (8-10 mm width and 15-20 mm length). Bipolar recordings were performed in the isthmus at a resolution of 1.2 mm and single extrastimuli with eight basic drive trains were delivered to cause conduction blocks in the isthmus. When a conduction block occurred, the recorded coupling interval at the recording site just proximal to the site of block was defined as BCI. At the site of the block, the ERP and duration of the monophasic action potential (MAP) at each drive cycle length was measured. The PRR was calculated using two different formulas: (1) [ERP-MAP] and (2) [BCI-MAP]. Procainamide was administrated intravenously at a dose of 15 mg/kg after the control study and the whole study protocol was repeated. The site of the block in an individual dog was always the same. BCI, ERP, and MAP were all shortened in accordance with the shortening of the basic drive cycle length, and the BCI was always the longest, ERP the middle, and the MAP was the shortest. The administration of procainamide prolonged each parameter, but the order of BCI > ERP > MAP remained unchanged. The PRR calculated as [BCI-MAP] was prolonged from 15 +/- 10 ms to 29 +/- 8 ms by the administration of procainamide (P = 0.048), but [ERP-MAP] was unchanged (8 +/- 10 ms vs 8 +/- 4 ms). In the conduction block model in the canine right atrium, procainamide prolonged the [BCI-MAP], but did not change the [ERP-MAP]. The procainamide effect of prolonging the PRR might be expressed better by the change in the BCI than the ERP.
authors.affiliation: Department of Internal Medicine, Kitasato University School of Medicine, 15-1 Kitasato 1, Sagamihara, 228-8555 Japan. naotoy@med.kitasato-u.ac.jp
authors.forename: N
authors.initials: N
authors.lastname: Yoshizawa
authors.forename: S
authors.initials: S
authors.lastname: Niwano
authors.forename: M
authors.initials: M
authors.lastname: Moriguchi
authors.forename: Y
authors.initials: Y
authors.lastname: Kitano
authors.forename: K
authors.initials: K
authors.lastname: Inuo
authors.forename: J
authors.initials: J
authors.lastname: Saito
authors.forename: T
authors.initials: T
authors.lastname: Izumi
mesh_terms: D000818:Animals; D000889:Anti-Arrhythmia Agents / Q000494:pharmacology*; D004195:Disease Models, Animal; D004285:Dogs; D004594:Electrophysiology; D006327:Heart Block / Q000503:physiopathology*; D006329:Heart Conduction System / Q000187:drug effects* / Q000503:physiopathology*; D011342:Procainamide / Q000494:pharmacology*; D012032:Refractory Period, Electrophysiological / Q000187:drug effects*
chemical_list: D000889:Anti-Arrhythmia Agents; D011342:Procainamide