title: In vitro culture of human epithelial cells on a modified xenogenic collagen support.
abstract: Human epithelial cells (HeLa, HaCaT, NHK) were cultured in vitro on chemically modified collagen membranes. Adhesion to the support was measured by estimation of the percentage of adhering 51Cr-labeled cells. Proliferation was estimated with the XTT test. Morphological observations of cells growing on HCl-treated collagen were performed using histological and electron microscopic techniques. HCl and trypsin-modified xenogenic collagen was found to be a good support for human cells in vitro. EDTA-incubated collagen enhanced neither adhesion nor proliferation. The best adhesion and proliferation were found on HCl-treated collagen, depending, however, on the kind of cells.
authors.affiliation: Department of Transplantology and Central Tissue Bank, Centre of Biostructure Research, Warsaw Medical University, Poland. elesiak@ib.amwaw.edu.pl
authors.forename: E
authors.initials: E
authors.lastname: Lesiak-Cyganowska
authors.forename: D
authors.initials: D
authors.lastname: Sladowski
authors.forename: E
authors.initials: E
authors.lastname: Jankowska
authors.forename: J
authors.initials: J
authors.lastname: Komender
mesh_terms: D002448:Cell Adhesion; D002455:Cell Division; D002478:Cells, Cultured; D003094:Collagen / Q000494:pharmacology*; D004492:Edetic Acid / Q000494:pharmacology; D004847:Epithelial Cells / Q000166:cytology / Q000502:physiology; D006801:Humans; D006851:Hydrochloric Acid / Q000494:pharmacology; D014357:Trypsin / Q000494:pharmacology
chemical_list: D003094:Collagen; D004492:Edetic Acid; D014357:Trypsin; D006851:Hydrochloric Acid