[Detection of monosomy 7 or 7q- in cases of myelodysplastic syndrome].
OBJECTIVE
To investigate the value of interphase fluorescence in situ hybridization(FISH) in the detection of monosomy 7 (-7)or deletion of long arm of chromosome 7(7q-) in the cases of myelodysplastic syndrome (MDS).


METHODS
Forty-six cases of MDS and 10 normal controls were studied simultaneously by conventional karyotype analysis and interphase FISH technique using SpectrumRed directly labeled DNA specific probe for 7q32. Two hundred interphase cells were analyzed for each case and the cells with one red hybridization spot<7% were regarded as positive.


RESULTS
Three cases displayed -7/7q- by conventional cytogenetics(CC) and were confirmed by interphase FISH. Six cases in 43 cases who did not show -7/7q- by CC displayed -7/7q- by interphase FISH.


CONCLUSIONS
Interphase FISH is very useful for the detection of -7 or 7q- in MDS and it is more sensitive than CC.
Shen
Y
Y
First Affiliated Hospital of Suzhou University, Jiangsu Institute of Hematology, Suzhou, Jiangsu 215006 P.R.China. uujihsmc@public1.sz.js.cn
Xue
Y
Y
Li
J
J
Guo
Y
Y
Pan
J
J
Wu
Y
Y
D000293:Adolescent; D000328:Adult; D002869:Chromosome Aberrations; D002871:Chromosome Banding; D002897:Chromosomes, Human, Pair 7 / Q000235:genetics*; D020732:Cytogenetic Analysis; D015342:DNA Probes; D005260:Female; D006801:Humans; D017403:In Situ Hybridization; D007621:Karyotyping; D008297:Male; D008875:Middle Aged; D009006:Monosomy; D009190:Myelodysplastic Syndromes / Q000235:genetics*; D055815:Young Adult
D015342:DNA Probes