Genetic basis of mitochondrial HMG-CoA synthase deficiency.
Deficiency of mitochondrial 3-hydroxy-3-methylglutaryl-CoA synthase (mHMGS) is a recessive disorder of ketogenesis that has been previously diagnosed in two children with hypoglycaemic hypoketotic coma during fasting periods. Here, we report the results of molecular investigations in a third patient affected by this disease. Sequencing of the entire coding region of the HMGCS2 gene revealed two missense mutations, G212R and R500H. Mendelian inheritance was confirmed by the analysis of parental samples and neither of the mutations was found on 200 control chromosomes. Functional relevance was confirmed by in vitro expression studies in cytosolic HMGS-deficient cells. Whereas wild-type cDNA of the HMGCS2 gene reverted the auxotrophy for mevalonate, the cDNAs of the mutants did not. The disease may be recognised by specific clinical and biochemical features but it is difficult to confirm enzymatically since the gene is expressed only in liver and testis. Molecular studies may facilitate or confirm future diagnoses in affected patients.
Aledo
R
R
Department of Molecular Biology, International University of Catalonia, Barcelona, Spain.
Zschocke
J
J
Pié
J
J
Mir
C
C
Fiesel
S
S
Mayatepek
E
E
Hoffmann
G F
GF
Casals
N
N
Hegardt
F G
FG
D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D006224:Cricetinae; D004252:DNA Mutational Analysis; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D005260:Female; D015870:Gene Expression; D005808:Genes, Recessive; D006801:Humans; D006904:Hydroxymethylglutaryl-CoA Synthase / Q000172:deficiency* / Q000235:genetics*; D066298:In Vitro Techniques; D007223:Infant; D008297:Male; D008928:Mitochondria / Q000201:enzymology*; D016297:Mutagenesis, Site-Directed; D020125:Mutation, Missense
D017931:DNA Primers; D018076:DNA, Complementary; D006904:Hydroxymethylglutaryl-CoA Synthase