[Biological activity of recombinant human hepatopoietin].
We examined the effects of rhHPO on the cell growth and DNA synthesis of both rat primarily cultured hepatocytes and hepatic carcinoma cell line in vitro by MTS and 3H-TdR in corporation methods. It was indicated that rhHPO is an important stimulating factor of regeneration, which may be developed as a potential drug for the treatment of severe hepatic diseases. We also found an inhibitory effect of rhHPO on the DNA synthesis of lung cancer cell lines GLC-82 in vitro, which might provide a valuable indicator for the study of its specificity and mechanisms.
Zhang
Y
Y
Institute of Radiation Medicine, Beijing 100850.
Yang
X M
XM
Wang
G
G
He
F C
FC
D000818:Animals; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D017228:Hepatocyte Growth Factor / Q000494:pharmacology*; D022781:Hepatocytes / Q000166:cytology*; D008113:Liver Neoplasms / Q000473:pathology*; D008115:Liver Regeneration / Q000187:drug effects*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D011994:Recombinant Proteins; D017228:Hepatocyte Growth Factor; D004247:DNA