The identification of the sympathetic neurons innervating the hamster submandibular gland and their electrophysiological membrane properties.
The neuron innervating the hamster submandibular (SM) gland was identified in the superior cervical ganglion (SCG) in vitro by recording the antidromic response using the intracellular recording technique. After the cellular response was recorded, methylene blue was injected iontophoretically into the neuron from the recording electrode, and the location of the cell soma was determined. The salivatory neurons of the SM gland were in the small- to medium-sized group of the entire cell population of the SCG. The cell size was 36.3 x 24.4 microm (mean, n=45). The postganglionic fibers were entirely unmyelinated (mean: 0.34 m/sec at 28-30 degrees C, n=141). Eighty-seven percent of the cells were distributed in the central one-third of area between the external carotid nerve origin and the caudal pole in the SCG. The resting membrane potential, membrane input resistance, membrane time constant and membrane input capacitance of the salivatory neuron were as follows: -49.2+/-7.6 mV (n=102), 52.9+/-23.6 Mohms (n=71), 8.0+/-3.4 msec (n=71) and 147+/-50 pF (n=71). Fast- and slow-excitatory postsynaptic potentials (EPSPs) were evoked, but not slow-inhibitory postsynaptic potentials (IPSPs). The fast EPSP was 13.1+/-5.7 mV in amplitude and 46.2+/-17.1 msec in duration (n=35). The slow EPSP (20 Hz, 5 sec) was 6.9+/-11 .9 mV in amplitude and 101+/-43 sec in duration (n=16). The directly-evoked spike was 63.0+/-11.9 mV in amplitude and 5.9+/-1.3 msec in duration (n=54). The spike after-hyperpolarization (AHP) was 12.5+/-3.5 mV in amplitude and 353+/-161 msec in duration. Na+ and Ca+ channels were involved in the spike generation. The voltage-dependent K+ channels (delayed rectifier), A channels and rapidly Ca2+-activated K+ channels (BK channels) regulated the spike-falling phase. The delayed rectifiers, A channels, and BK and SK (slowly Ca2+-activated) channels were involved in generation of spike-AHP. Muscarine suppressed the Ca2+ component of spike via muscarinic receptors.
Morita
M
M
Department of Physiology, Tokyo Dental College, Chiba, Japan.
Suzuki
T
T
D015761:4-Aminopyridine / Q000494:pharmacology; D000818:Animals; D015220:Calcium Channels / Q000187:drug effects / Q000502:physiology; D048429:Cell Size; D004396:Coloring Agents; D006224:Cricetinae; D004553:Electric Conductivity; D017097:Electric Impedance; D004567:Electrodes, Implanted; D004594:Electrophysiology; D005071:Evoked Potentials / Q000187:drug effects / Q000502:physiology; D019706:Excitatory Postsynaptic Potentials / Q000502:physiology; D007478:Iontophoresis; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology*; D008751:Methylene Blue; D008839:Microelectrodes; D009116:Muscarine / Q000494:pharmacology; D018721:Muscarinic Agonists / Q000494:pharmacology; D009412:Nerve Fibers / Q000187:drug effects / Q000648:ultrastructure; D009431:Neural Conduction / Q000187:drug effects; D009474:Neurons / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure*; D026902:Potassium Channel Blockers; D015221:Potassium Channels / Q000502:physiology; D011976:Receptors, Muscarinic / Q000187:drug effects; D015222:Sodium Channels / Q000502:physiology; D013363:Submandibular Gland / Q000294:innervation*; D017783:Superior Cervical Ganglion / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure; D013564:Sympathetic Nervous System / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure*; D013997:Time Factors
D015220:Calcium Channels; D004396:Coloring Agents; D018721:Muscarinic Agonists; D026902:Potassium Channel Blockers; D015221:Potassium Channels; D011976:Receptors, Muscarinic; D015222:Sodium Channels; D009116:Muscarine; D015761:4-Aminopyridine; D008751:Methylene Blue