title: RETRACTED: Lysophosphatidylcholine as a ligand for the immunoregulatory receptor G2A.
abstract: Although the biological actions of the cell membrane and serum lipid lysophosphatidylcholine (LPC) in atherosclerosis and systemic autoimmune disease are well recognized, LPC has not been linked to a specific cell-surface receptor. We show that LPC is a high-affinity ligand for G2A, a lymphocyte-expressed G protein-coupled receptor whose genetic ablation results in the development of autoimmunity. Activation of G2A by LPC increased intracellular calcium concentration, induced receptor internalization, activated ERK mitogen-activated protein kinase, and modified migratory responses of Jurkat T lymphocytes. This finding implicates a role for LPC-G2A interaction in the etiology of inflammatory autoimmune disease and atherosclerosis.
authors.affiliation: Department of Microbiology, Immunology, and Molecular Genetics, Lerner Research Institute, 9500 Euclid Avenue, Cleveland, OH 44195, USA.
authors.forename: J H
authors.initials: JH
authors.lastname: Kabarowski
authors.forename: K
authors.initials: K
authors.lastname: Zhu
authors.forename: L Q
authors.initials: LQ
authors.lastname: Le
authors.forename: O N
authors.initials: ON
authors.lastname: Witte
authors.forename: Y
authors.initials: Y
authors.lastname: Xu
mesh_terms: D000818:Animals; D001161:Arteriosclerosis / Q000276:immunology / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000378:metabolism*; D002460:Cell Line; D002634:Chemotaxis, Leukocyte; D006801:Humans; D019169:Jurkat Cells; D008024:Ligands; D008180:Lupus Erythematosus, Systemic / Q000276:immunology / Q000378:metabolism; D008213:Lymphocyte Activation; D008244:Lysophosphatidylcholines / Q000378:metabolism* / Q000494:pharmacology; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D010767:Phosphorylcholine / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology; D011869:Radioligand Assay; D011956:Receptors, Cell Surface / Q000378:metabolism*; D043562:Receptors, G-Protein-Coupled; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D015398:Signal Transduction; D013110:Sphingosine / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism* / Q000502:physiology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014162:Transfection; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
chemical_list: D018797:Cell Cycle Proteins; C115270:G2A receptor; D008024:Ligands; D008244:Lysophosphatidylcholines; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; D011993:Recombinant Fusion Proteins; D010566:Virulence Factors, Bordetella; C005356:sphingosine phosphorylcholine; D010767:Phosphorylcholine; D020928:Mitogen-Activated Protein Kinases; D013110:Sphingosine; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium