title: [The arboviruses of genus alphavirus family togaviridae were isolated from mosquitoes captured from Yantai].
abstract: OBJECTIVE
  To isolate arbovirus from mosquitoes caught from Yantai.
  
  
  METHODS
  The isolated viruses were tested for their physico- chemical properties, examined by electron microscopy and specific immuno-reactivity.
  
  
  RESULTS
  Fifteen strains of virus were isolated from mosquitoes in 1994 from Yantai. Three of them were further assayed. The results showed that the viruses could multiply on C6/36 cell and produce typical cytopathogenic effect. The viruses couldn't cause regular sickness and death of suckling mice by intracerebral inoculation. The viruses were sensitive to ether, but resistant to 5, -Idu. One sample was examined with electron microscope, and spherical virus particles were observed. The diameter of the virus particles is about 55 +/- 2. 3nm. It did not react with the JBE virus and Bunyaviridae group specific immuno-ascitic fluids but cross-reacted with the group A Togaviridae specific immuno-ascitic fluid.
  
  
  CONCLUSIONS
  The results showed that the viruses isolated from Yantai belong to alphavirus of togaviridae.
authors.affiliation: Yantai Health and Anti-epidemic Station, Yantai 264001, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.forename: S
authors.initials: S
authors.lastname: Yang
authors.forename: Y
authors.initials: Y
authors.lastname: Sun
mesh_terms: D000818:Animals; D001103:Arboviruses / Q000276:immunology / Q000302:isolation & purification* / Q000648:ultrastructure; D002478:Cells, Cultured; D009033:Culicidae / Q000821:virology*; D051379:Mice; D014035:Togaviridae / Q000276:immunology / Q000302:isolation & purification* / Q000648:ultrastructure