Establishment of a swine monocyte cell line.
A swine monocyte cell line was established from peripheral blood sample collected from a healthy adult male pig. The cloned cells grow actively in forming monolayers in both glass and plastic cell culture flasks with the growth medium reported previously (Kadoi, 2000) at 36.5 degrees C incubation. The plating efficiency is more than 95%. Densely grown cells in flasks show an epithelioid morphology. The fundamental properties of the cells were examined for cytological definition as monocytes. A positive property detected was guinea pig complement receptor, porcine IgG receptor, non-specific esterase, and acid phosphatase. A significant phagocytic activity proved by the inoculation of Saccharomyces cerevisiae is also one of the characteristics observed in the LPS-activated cells.
Kadoi
K
K
College of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa, Japan.
Tsukise
A
A
Shiba
H
H
Ikeda
K
K
Seki
T
T
Ariga
T
T
D000818:Animals; D015703:Antigens, CD; D015415:Biomarkers / Q000032:analysis; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation / Q000187:drug effects; D048429:Cell Size; D002478:Cells, Cultured; D002999:Clone Cells / Q000166:cytology / Q000187:drug effects / Q000201:enzymology / Q000276:immunology; D007621:Karyotyping; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D009000:Monocytes / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology / Q000276:immunology; D010587:Phagocytosis / Q000187:drug effects; D011951:Receptors, Complement / Q000032:analysis; D012441:Saccharomyces cerevisiae / Q000276:immunology; D013552:Swine
D015703:Antigens, CD; D015415:Biomarkers; D008070:Lipopolysaccharides; D011951:Receptors, Complement