Determination of moclobemide, paroxetine, and fluvoxamine in tablets by HPLC.
A simple, accurate high performance liquid chromatography procedure is presented in order to determine moclobemide in Aurorix-tablets, paroxetine in Seroxat-tablets and fluvoxamine in Fevarin tablets. These drugs were chromatographed on a Nova-Pak C18, (dp = 4 microm), 150x3.9 mm i.d. column using methanol/phosphate butter adjusted to pH 2.65 with phosphoric acid (7:3, v/v) as the mobile phase to determining of the moclobemide and methanol/tetrahydrofuran/phosphate buffer at pH 2.65 (53:5:4, v/v) for determining of paroxetine and fluvoxamine. The detection was carried at 235 nm. The method was tested for linearity over the range 5-50 microg/ml. The relative standard deviation for moclobemide is 1.16, but for paroxetine and fluvoxamine is less than 1% (0.25 and 0.46 respectively).
Skibiński
R
R
Department of Medicinal Chemistry, Medical Academy, Lublin, Poland.
Misztal
G
G
D000928:Antidepressive Agents / Q000032:analysis*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D016666:Fluvoxamine / Q000032:analysis*; D020912:Moclobemide / Q000032:analysis*; D017374:Paroxetine / Q000032:analysis*; D013607:Tablets
D000928:Antidepressive Agents; D013607:Tablets; D017374:Paroxetine; D016666:Fluvoxamine; D020912:Moclobemide