Expression of neurotrophin receptors in rat testis. Upregulation of TrkA mRNA with hCG treatment.
We report the expression of TrkA, TrkB and TrkC mRNAs in adult rat testis. With in situ hybridisation a low signal for TrkB and TrkC could be seen in postmeiotic cells of the seminiferous epithelium, whereas no signal for TrkA could be observed in untreated animals. Animals treated with hCG showed an induction of TrkA mRNA in premeiotic cells 12 h after the treatment, whereas an injection with EDS had no effect on the expression of Trk mRNAs. With the RNAse protection assay a low signal for TrkA was seen in whole testis of hCG treated animals. In staged tubules low expression was seen at stages VII-XI of untreated animals. Animals injected with hCG revealed that TrkA induction was highest during stages VIIcd and VIII of the cycle. The distinct expression pattern of these high-affinity neurotrophin receptors suggests different roles for neurotrophins during spermatogenesis. Induction of TrkA mRNA by hCG suggests that high-affinity binding of NGF during stages VIIcd-VIII in premeiotic cells is under control of the hypothalamic-pituitary-testicular axis.
Schultz
R
R
Department of Developmental Biology, University of Tampere, 33014 Tampere, Finland.
Metsis
M
M
Hökfelt
T
T
Parvinen
M
M
Pelto-Huikko
M
M
D000818:Animals; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D017403:In Situ Hybridization; D008297:Male; D008698:Mesylates / Q000494:pharmacology; D012333:RNA, Messenger / Q000032:analysis / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D020917:Receptor, trkA / Q000235:genetics / Q000378:metabolism; D020813:Receptor, trkB / Q000235:genetics / Q000378:metabolism; D020812:Receptor, trkC / Q000235:genetics / Q000378:metabolism; D017475:Receptors, Nerve Growth Factor / Q000235:genetics / Q000378:metabolism*; D012670:Seminiferous Epithelium / Q000737:chemistry; D013737:Testis / Q000737:chemistry* / Q000166:cytology; D015854:Up-Regulation / Q000187:drug effects
D006063:Chorionic Gonadotropin; D008698:Mesylates; D012333:RNA, Messenger; D017475:Receptors, Nerve Growth Factor; D020917:Receptor, trkA; D020813:Receptor, trkB; D020812:Receptor, trkC; C002994:ethylene dimethanesulfonate