Microchip isoelectric focusing using a miniature scanning detection system.
A miniature scanning fluorescent detector has been developed for plastic microchannel isoelectric focusing (mIEF) analysis. The detector, comprised of a lamp and photomultiplier tube (PMT) on a moving stage, measured the real-time distribution of fluorescently labeled peptides subjected to gel-free mIEF. During the run, the effective length of the 6-cm channel was scanned every 9 s. Analysis was completed within 5 min while still obtaining high resolution and sensitivity. In addition, the scanning detector was used to characterize peptide migration properties within the channel by providing simultaneous temporal and spatial measurements.
Raisi
F
F
Cepheid, Sunnyvale, CA 98089, USA.
Belgrader
P
P
Borkholder
D A
DA
Herr
A E
AE
Kintz
G J
GJ
Pourhamadi
F
F
Taylor
M T
MT
Northrup
M A
MA
D000595:Amino Acid Sequence; D004867:Equipment Design; D005456:Fluorescent Dyes; D007525:Isoelectric Focusing / Q000295:instrumentation* / Q000379:methods*; D009842:Oligopeptides / Q000032:analysis / Q000737:chemistry; D012235:Rhodamines; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods
D005456:Fluorescent Dyes; D009842:Oligopeptides; D012235:Rhodamines