Micronuclei and chromatid buds are the result of related genotoxic events.
Chromatin buds (CHB), broken eggs, or budding cell nuclei are structures similar to micronuclei (MN) in shape, structure, and size, which are linked to the main nuclei of cells by a thread or stalks of chromatin. They have been observed in numerous cell types and there are reports of their existence relating them with MN or with genotoxic events. However, there is no systematic study reporting their frequency and no experiment has been done to ascertain whether they are really induced by genotoxins. Furthermore, they have been discarded as genotoxic events with the argument that they are not formed in dividing cells. Studies are presented here that indicate that CHB can be considered as genotoxic events and that their origin is comparable to that of MN. Bromodeoxyuridine (BrdU) was used to label proliferating lymphocytes, which were later identified by means of an immunohistochemical method, using the H2O2-DAB stain. The results show that CHB are consistently formed where MN are seen. CHB were induced by the clastogen mitomycin C (MMC) as well as by the aneuploidogen colcemid, with frequencies similar to MN in both cases, and to multinucleated cells in the case of colcemid. CHB occur in lymphocytes of smokers with frequencies similar to those of MN, and we found that the infection with Taenia solium metacestodes induced a comparable increase of both MN and CHB frequency in lymphocytes from pigs.
Serrano-García
L
L
Departamento de Toxicología Genética y Ambiental, Instituto de Investigaciones Biomédicas, UNAM, Distrito Federal, Mexico.
Montero-Montoya
R
R
D000782:Aneuploidy; D000818:Animals; D002842:Chromatids / Q000648:ultrastructure*; D003703:Demecolcine / Q000633:toxicity*; D006801:Humans; D048629:Micronuclei, Chromosome-Defective; D016685:Mitomycin / Q000633:toxicity*; D009153:Mutagens / Q000633:toxicity*; D013552:Swine; D013621:Taenia / Q000472:pathogenicity
D009153:Mutagens; D016685:Mitomycin; D003703:Demecolcine