Influence of n-alkanes and petroleum on fatty acid composition of a hydrocarbonoclastic bacterium: Marinobacter hydrocarbonoclasticus strain 617.
This study concerns the effects of various long-chain n-alkanes, n-alkane mixtures and Arabian Light crude oil on the fatty acid (FA) composition of a sedimentary marine bacteria (Marinobacter hydrocarbonoclasticus strain 617), growing under aerobic conditions. The cultures with n-alkanes, as compared with soluble carbon sources, led to greater amounts of saturated and methyl branched FA (mainly belonging to a delta10 series). We observed the appearance or increase of saturated and unsaturated FA with the same carbon chain length (CCL) as the n-alkane carbon source (maximum for n-alkane CCL corresponding to the 'range' of the de novo synthesized fatty acids). We also observed a strong control of the oddness/evenness of the CCL of the FA by the oddness/evenness of the n-alkane. A n-alkane utilization index, (saturated + branched)/monounsaturated fatty acids (SAFA + BFA/MUFA) enabled discriminating between soluble carbon sources and hydrocarbons.
Doumenq
P
P
Laboratoire de Chimie Analytique de l'Environnement, Faculté des Sciences et Techniques de St Jérome, Université d'Aix Marseille, UPRES A 6171, Marseille , France. pierre.doumenq@csmlcae.u-3mrs.fr
Aries
E
E
Asia
L
L
Acquaviva
M
M
Artaud
J
J
Gilewicz
M
M
Mille
G
G
Bertrand
J C
JC
D000473:Alkanes / Q000737:chemistry*; D001419:Bacteria / Q000737:chemistry*; D005227:Fatty Acids / Q000032:analysis / Q000096:biosynthesis*; D010578:Petroleum / Q000009:adverse effects*; D014876:Water Pollution / Q000009:adverse effects*
D000473:Alkanes; D005227:Fatty Acids; D010578:Petroleum