title: Release of IL-4 and IL-5 from human peripheral blood mononuclear cells co-cultured with Japanese cedar pollen antigen in vitro.
abstract: The Japanese cedar pollen (JCP) is a major allergen with respect to pollinosis in Japan. It is believed that interleukin-4 (IL-4) and interleukin-5 (IL-5) derived from lymphocytes and other cells play a pivotal role in allergic reactions. We investigated whether the JCP antigen stimulates the release of these cytokines by peripheral blood mononuclear cells (PBMCs). PBMCs from eight adults (five adults with JCP pollinosis and three adults without JCP pollinosis) were co-incubated with purified JCP antigens. IL-4 was released in response to JCP antigens in six of the eight subjects at 24 h and in three subjects at 48 h. IL-4 release at 24 h occurred in all five subjects with JCP pollinosis but in only one of the three subjects without pollinosis. IL-5 was released in response to the JCP antigen in five of the eight subjects at 24 h and 48 h, including four of the five subjects with JCP pollinosis and one of the three subjects without pollinosis. These results suggest that PBMCs were more likely to release IL-4 and IL-5 in the presence of JCP pollinosis.
authors.affiliation: Gunma Prefectural Institute of Public Health and Environmental Sciences, Maebashi, Gunma, 371-0052, Japan. kimura-hi@pref.gunma.jp
authors.forename: H
authors.initials: H
authors.lastname: Kimura
authors.forename: Y
authors.initials: Y
authors.lastname: Shibata
authors.forename: H
authors.initials: H
authors.lastname: Minakami
authors.forename: S
authors.initials: S
authors.lastname: Hashizume
authors.forename: H
authors.initials: H
authors.lastname: Oba
authors.forename: M
authors.initials: M
authors.lastname: Sakaguchi
authors.forename: K
authors.initials: K
authors.lastname: Otsuki
authors.forename: M
authors.initials: M
authors.lastname: Kato
mesh_terms: D000328:Adult; D000485:Allergens / Q000494:pharmacology*; D017077:Culture Media, Conditioned / Q000737:chemistry / Q000378:metabolism; D005260:Female; D006801:Humans; D015847:Interleukin-4 / Q000378:metabolism*; D015848:Interleukin-5 / Q000378:metabolism*; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D011058:Pollen / Q000276:immunology*; D006255:Rhinitis, Allergic, Seasonal / Q000097:blood / Q000276:immunology; D013997:Time Factors; D014197:Trees
chemical_list: D000485:Allergens; D017077:Culture Media, Conditioned; D015848:Interleukin-5; D015847:Interleukin-4