title: Cryptosporidium parvum infection requires host cell actin polymerization.
abstract: The intracellular protozoan parasite Cryptosporidium parvum accumulates host cell actin at the interface between the parasite and the host cell cytoplasm. Here we show that the actin polymerizing proteins Arp2/3, vasodilator-stimulated phosphoprotein (VASP), and neural Wiskott Aldrich syndrome protein (N-WASP) are present at this interface and that host cell actin polymerization is necessary for parasite infection.
authors.affiliation: Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.
authors.forename: D A
authors.initials: DA
authors.lastname: Elliott
authors.forename: D J
authors.initials: DJ
authors.lastname: Coleman
authors.forename: M A
authors.initials: MA
authors.lastname: Lane
authors.forename: R C
authors.initials: RC
authors.lastname: May
authors.forename: L M
authors.initials: LM
authors.lastname: Machesky
authors.forename: D P
authors.initials: DP
authors.lastname: Clark
mesh_terms: D051377:Actin-Related Protein 2; D051378:Actin-Related Protein 3; D000199:Actins / Q000378:metabolism*; D000818:Animals; D015815:Cell Adhesion Molecules / Q000378:metabolism; D002460:Cell Line; D003457:Cryptosporidiosis / Q000469:parasitology*; D016785:Cryptosporidium parvum / Q000472:pathogenicity*; D003598:Cytoskeletal Proteins; D006790:Host-Parasite Interactions; D006801:Humans; D008840:Microfilament Proteins; D009419:Nerve Tissue Proteins / Q000378:metabolism; D010750:Phosphoproteins / Q000378:metabolism; D011108:Polymers; D051317:Wiskott-Aldrich Syndrome Protein, Neuronal; D000098662:Vasodilator-Stimulated Phosphoprotein
chemical_list: C495926:ACTR2 protein, human; C495928:ACTR3 protein, human; D051377:Actin-Related Protein 2; D051378:Actin-Related Protein 3; D000199:Actins; D015815:Cell Adhesion Molecules; D003598:Cytoskeletal Proteins; D008840:Microfilament Proteins; D009419:Nerve Tissue Proteins; D010750:Phosphoproteins; D011108:Polymers; C495771:WASL protein, human; D051317:Wiskott-Aldrich Syndrome Protein, Neuronal; D000098662:Vasodilator-Stimulated Phosphoprotein