title: Purification and functional assay of pluripotent hematopoietic stem cells.
abstract: Hematolymphopoietic stem cells (HSC) have the capacity for extensive self-renewal and pluripotent myelolymphoid differentiation. Recent studies have emphasized the heterogeneity of human HSC subsets in terms of proliferative and self-renewal capacity. In the NOD-SCID (nonobese diabetic-severe combined immunodeficient) mouse xenograft assay, most CD34+38- stem cell clones proliferate at early times, but then disappear, whereas only few clones persist: possibly, the latter ones consist of long-term engrafting CD34+38- HSC expressing the KDR receptor (i.e. the vascular endothelial growth factor receptor II). In this regard, isolation of the small KDR+ subset from the CD34+ hematopoietic progenitors (and possibly from the CD34-lin- population) may provide a novel and effective approach for the purification of long-term proliferating HSC. More importantly, KDR+ HSC isolation will pave the way to cellular/molecular characterization and improved functional manipulation of HSC/HSC subsets, as well as to innovative approaches for HSC clinical utilization, specifically transplantation, transfusion medicine and gene therapy.
authors.affiliation: T. Jefferson University, Kimmel Cancer Center, Bluemle Life Science Building, Room 609, 233 South 10th Street, Philadelphia, PA 19107-5541, USA. cesare.peschle@mail.tju.edu
authors.forename: C
authors.initials: C
authors.lastname: Peschle
authors.forename: R
authors.initials: R
authors.lastname: Botta
authors.forename: R
authors.initials: R
authors.lastname: Müller
authors.forename: M
authors.initials: M
authors.lastname: Valtieri
authors.forename: B L
authors.initials: BL
authors.lastname: Ziegler
mesh_terms: D000818:Animals; D018952:Antigens, CD34; D015415:Biomarkers; D019070:Cell Lineage / Q000276:immunology; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000276:immunology; D006801:Humans; D020794:Receptor Protein-Tyrosine Kinases; D017978:Receptors, Growth Factor; D040262:Receptors, Vascular Endothelial Growth Factor
chemical_list: D018952:Antigens, CD34; D015415:Biomarkers; D017978:Receptors, Growth Factor; D020794:Receptor Protein-Tyrosine Kinases; D040262:Receptors, Vascular Endothelial Growth Factor