[Preparation of polyclonal antibody to human prion protein using the expressed GST-PrP fusion protein as antigen].
OBJECTIVE
Preparing specific antibody to prion protein.


METHODS
Using prokaryotic expressed GST-PrP fusion protein as antigen, rabbits were immunized subcutaneously.


RESULTS
ELISA assay revealed that the titer of the prepared antiserum against expressed PrP protein was as high as 1:128 000. Western blot test showed that the antiserum was able to react with the in vitro expressed intact and different lengths of C-terminus truncated PrP proteins, as well as the native PrP proteins from brain homogenization of human and mouse.


CONCLUSIONS
The prokaryotic expressed GST-PrP fusion protein can efficiently elicit in immunized animals the PrP-specific antibody.
Zhao
X
X
Institute of Virology, Chinese Academy of Preventive Medicine, Beijing 100052, China.
Dong
X
X
Zhou
W
W
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis*; D002478:Cells, Cultured; D006801:Humans; D051379:Mice; D011328:Prions / Q000032:analysis / Q000096:biosynthesis / Q000276:immunology*; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000276:immunology*
D000914:Antibodies, Viral; D011328:Prions; D011993:Recombinant Fusion Proteins