title: Ethanol exposure inhibits the cytotoxic effect induced by gp120 in CHP100 human neuroblastoma cells.
abstract: The aim of present study was to investigate the acute effects of ethanol on cytotoxicity induced by HIV-1 coat protein gp120 in CHP100 human neuroblastoma cell line. We demonstrate that ethanol, within a range of clinically relevant concentrations (15-90 mM) prevents cell death elicited by gp120 (10 pM) in a dose dependent manner. This protective action seems to be mediated by a reduction of free intracellular Ca(2+) levels because ethanol, at concentrations ranging from 0.1-0.5%, is able to decrease gp120-stimulated Ca(2+) uptake up to 24%. Furthermore, our data show an involvement of NO/cGMP messenger system pathway, because ethanol is also able to reduce gp120-stimulated NO release (up to 45%) and cyclic GMP accumulation (up to 73%). These findings suggest that the protective effect of ethanol against gp120-induced cytotoxicity in CHP100 cells underlies a Ca(2+)-activated, NO/cGMP-dependent mechanism.
authors.affiliation: Department of Pharmacobiological Sciences, Faculty of Pharmacy, University of Catanzaro Magna Graecia & IBAF-CNR, 88021 Roccelletta di Borgia, Catanzaro, Italy. navarra@unicz.it
authors.forename: M
authors.initials: M
authors.lastname: Navarra
authors.forename: C
authors.initials: C
authors.lastname: Romano
authors.forename: T
authors.initials: T
authors.lastname: Lorenzon
authors.forename: D
authors.initials: D
authors.lastname: Rotiroti
authors.forename: G
authors.initials: G
authors.lastname: Di Renzo
mesh_terms: D002118:Calcium / Q000378:metabolism; D016923:Cell Death / Q000187:drug effects; D002492:Central Nervous System Depressants / Q000494:pharmacology*; D006152:Cyclic GMP / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000494:pharmacology*; D018690:Excitatory Amino Acid Agonists / Q000494:pharmacology; D015699:HIV Envelope Protein gp120 / Q000633:toxicity*; D006801:Humans; D016202:N-Methylaspartate / Q000494:pharmacology; D009447:Neuroblastoma; D009474:Neurons / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D014407:Tumor Cells, Cultured
chemical_list: D002492:Central Nervous System Depressants; D018690:Excitatory Amino Acid Agonists; D015699:HIV Envelope Protein gp120; D009569:Nitric Oxide; D000431:Ethanol; D016202:N-Methylaspartate; D006152:Cyclic GMP; D002118:Calcium