Second-generation Hybrid capture test and Amplicor monitor test generate highly correlated hepatitis B virus DNA levels.
The performance of the Digene Hybrid Capture II HBV DNA Test HC II and the Roche Cobas Amplicor Monitor Test (Cobas-HBV) was evaluated on 252 serum samples. One hundred and seventy-three samples were HBV DNA positive and 75 HBV DNA negative by both assays. Four samples were HBV DNA positive by Cobas-HBV only. Linear regression analysis showed that the HBV DNA concentrations obtained from both assays were significantly related (n=173, r=0.976, P<0.0001). The results of the study show that Hybrid capture II and Cobas-HBV could be used equally in the management for patients with chronic HBV infection.
Poljak
M
M
Medical Faculty of Ljubljana, Institute of Microbiology and Immunology, Zaloska 4, 1105, Ljubljana, Slovenia. mario.poljak@mf.uni-lj.si
Marin
I J
IJ
Seme
K
K
Brinovec
V
V
Maticic
M
M
Meglic-Volkar
J
J
Lesnicar
G
G
Vince
A
A
D001331:Automation; D004279:DNA, Viral / Q000097:blood*; D006515:Hepatitis B virus / Q000235:genetics* / Q000302:isolation & purification*; D019694:Hepatitis B, Chronic / Q000097:blood / Q000175:diagnosis* / Q000821:virology*; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014773:Virology / Q000379:methods
D004279:DNA, Viral