Nonlinear focusing of DNA macromolecules.
The present paper reports the nonlinear electrophoretic focusing techniques developed after an original idea by Chacron and Slater [Phys. Rev. E 56, 3436 (1997)]. Focusing of DNA molecules is achieved in an alternating nonuniform electric field, created in a wedge gel with hyperbolic boundaries. The fractions separated on such a wedge retained their rectilinear shape during the electrophoresis. Experiments with gel electrophoresis confirm the possibility of a noticeable nonlinear focusing of DNA molecules.
Frumin
L L
LL
Proteologics (Israel) Ltd., Weizmann Science Park, Building Lev HaPark, 5 Oppenheimer Street, Rehovot, 76701, Israel.
Peltek
S E
SE
Zilberstein
G V
GV
D010582:Bacteriophage lambda / Q000737:chemistry / Q000235:genetics; D003198:Computer Simulation; D004247:DNA / Q000737:chemistry* / Q000302:isolation & purification / Q000528:radiation effects*; D004279:DNA, Viral / Q000737:chemistry / Q000302:isolation & purification / Q000528:radiation effects; D004563:Electrochemistry / Q000379:methods; D004574:Electromagnetic Fields; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D008956:Models, Chemical; D008958:Models, Molecular; D017711:Nonlinear Dynamics; D012680:Sensitivity and Specificity
D004279:DNA, Viral; D004247:DNA