Structure of the O-specific polysaccharide of Proteus mirabilis D52 and typing of this strain to Proteus serogroup O33.
The acidic O-specific polysaccharide chain (O-antigen) of the lipopolysaccharide (LPS) of Proteus mirabilis strain D52 was studied using chemical analyses along with 1H-NMR and 13C-NMR spectroscopy, including 2D COSY, TOCSY, ROESY, H-detected 1H,13C and 1H,31P HMQC experiments. The polysaccharide was found to contain D-ribitol 5-phosphate (D-Rib-ol-5-P) and ethanolamine phosphate (Etn-P) and has the following structure: D-Rib-ol-5-P (3) approximately 75% EtnP(6)-->2)-beta-D-Galp-(1-->3)-alpha-D-GlcpNAc-(1-->3)-beta-D-Glcp-(1-->3)-beta-D-GlcpNAc-(1-->). This structure is identical with that of the O-polysaccharide of P. mirabilis O33 strain 59/57, and, hence, P. mirabilis D52 belongs to the same Proteus serogroup O33. Serological studies with O-antiserum against P. mirabilis D52 confirmed this but showed that the LPS species of P. mirabilis 59/57 and D52 are not identical, having different epitopes in the core region. A serological cross-reactivity of P. mirabilis D52 O-antiserum was observed with LPS of two other Proteus strains, P. mirabilis O16 and P. penneri 103, which have structurally different O-polysaccharides. The role of charged groups, Rib-ol-5-P and Etn-P in the immunospecificity is discussed.
Zych
K
K
Department of General Microbiology, Institute of Microbiology and Immunology, University of Lodz, Poland.
Toukach
F V
FV
Arbatsky
N P
NP
Kolodziejska
K
K
Senchenkova
S N
SN
Shashkov
A S
AS
Knirel
Y A
YA
Sidorczyk
Z
Z
D000818:Animals; D015153:Blotting, Western; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D004983:Ethanolamines / Q000737:chemistry; D006461:Hemolysis; D007124:Immunoenzyme Techniques; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008956:Models, Chemical; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000737:chemistry*; D011513:Proteus mirabilis / Q000737:chemistry*; D011817:Rabbits; D012255:Ribitol / Q000737:chemistry
D002241:Carbohydrates; D004983:Ethanolamines; D008070:Lipopolysaccharides; D011134:Polysaccharides; D012255:Ribitol; C008984:polyribitol phosphate; C005448:phosphorylethanolamine