title: Purification of nucleic acids by hybridization to affinity tagged PNA probes.
abstract: The use of affinity tagged PNA capture probes offers an efficient means for the purification of nucleic acids by hybridization. Two different approaches are described. A sequence specific method and a generic method. The sequence specific method requires sequence information on the target and synthesis of a dedicated PNA. It can be used to selectively purify the nucleic acid containing the target from non-related nucleic acids and other cellular components. The generic method uses a "universal" triplex forming PNA and requires no sequence information on the target. It can be used in the bulk purification of large nucleic acids.
authors.affiliation: PNA Diagnostics A/S, Rønnegade 2, 6. Floor, 2100 Copenhagen ø, Denmark.
authors.forename: H
authors.initials: H
authors.lastname: Ørum
mesh_terms: D006801:Humans; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D009696:Nucleic Acids / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D020135:Peptide Nucleic Acids / Q000235:genetics / Q000378:metabolism*
chemical_list: D009696:Nucleic Acids; D020135:Peptide Nucleic Acids