title: Src homology-2 domain binding assays by scintillation proximity and surface plasmon resonance.
abstract: Various SH2 competitive binding assays, based on different techniques, have been described in the literature to identify and characterize SH2 ligands. The consideration that most reported methods show experimental limitations associated with assay parameters has prompted us to base our Src-SH2 inhibitor discovery program on the use of two different assays. In this study, two conceptually different biochemical methods designed to discover Src-SH2 inhibitors, respectively scintillation proximity assay (SPA) and surface plasmon resonance (SPR), have been evaluated and compared. For its high sensitivity and adaptability to automation SPA was chosen for high capacity screening (primary screen), whereas SPR was used for hits confirmation (secondary screening). However with the drastic improvement of inhibitor affinities, the limit of sensitivity was rapidly reached for the SPR assay based on the canonical pYEEI ligand. The substitution of the natural, monophosphorylated peptide ligand with a triphosphorylated peptide has allowed us to remarkably increase its sensitivity, so that molecules with nanomolar affinities could be easily differentiated in terms of IC(50) ranking. Such a new, improved SPR assay can be of great interest for the study of high affinity ligands of different SH2-based drug targets.
authors.affiliation: Bone Disease Group, Hoechst Marion Roussel/Aventis, 102 Route de Noisy, 93235 Romainville Cedex, France. Eliane.Mandine@aventis.com
authors.forename: E
authors.initials: E
authors.lastname: Mandine
authors.forename: D
authors.initials: D
authors.lastname: Gofflo
authors.forename: V
authors.initials: V
authors.lastname: Jean-Baptiste
authors.forename: E
authors.initials: E
authors.lastname: Sarubbi
authors.forename: G
authors.initials: G
authors.lastname: Touyer
authors.forename: P
authors.initials: P
authors.lastname: Deprez
authors.forename: D
authors.initials: D
authors.lastname: Lesuisse
mesh_terms: D001667:Binding, Competitive; D019921:Biotinylation; D007700:Kinetics; D008024:Ligands; D010748:Phosphopeptides / Q000037:antagonists & inhibitors / Q000737:chemistry*; D011487:Protein Conformation; D012588:Scintillation Counting; D012680:Sensitivity and Specificity; D020349:Surface Plasmon Resonance / Q000379:methods*; D018909:src Homology Domains; D019061:src-Family Kinases / Q000037:antagonists & inhibitors / Q000737:chemistry
chemical_list: D008024:Ligands; D010748:Phosphopeptides; D019061:src-Family Kinases