title: Use of quantitative analysis of sonographic brightness for detection of early healing of tendon injury in horses.
abstract: OBJECTIVE
  To determine whether quantitative analysis of sonographic brightness could be used to detect healing of an induced injury of the superficial digital flexor tendon in horses and whether rate of healing was influenced by equine recombinant growth hormone.
  
  
  METHODS
  8 clinically normal Standardbreds.
  
  
  METHODS
  A localized injury was created in the left and right superficial digital flexor tendons of each horse by injection of 2,000 units of collagenase. After injury, 4 horses received equine recombinant growth hormone, a possible promoter of tendon healing. Sonographic images (7.5 MHz) of the flexor tendons and ligaments of the metacarpal region were recorded on videotape prior to injury and weekly for 7 weeks after injury. Images were digitized, and sonographic brightness of tendons and ligaments was calculated.
  
  
  RESULTS
  Collagenase-induced injury was sonographically similar to naturally occurring injury. After injury, sonographic brightness of the tendon decreased; after 3 weeks, brightness progressively increased, although by 7 weeks brightness had not returned to preinjury value. Equine recombinant growth hormone had no significant effect on the rate of tendon healing, as evaluated sonographically or at necropsy.
  
  
  CONCLUSIONS
  As healing developed, alterations in sonographic brightness of injured tendons coincided with real changes in tendon structure. Quantitative sonographic brightness could be used to accurately monitor healing of equine tendon and ligament injuries and investigate the efficacy of various treatment regimens.
authors.affiliation: Department of Veterinary Clinical Sciences, University of Sydney, Camden, NSW, Australia.
authors.forename: L
authors.initials: L
authors.lastname: Micklethwaite
authors.forename: A K
authors.initials: AK
authors.lastname: Wood
authors.forename: C M
authors.initials: CM
authors.lastname: Sehgal
authors.forename: M
authors.initials: M
authors.lastname: Polansky
authors.forename: B A
authors.initials: BA
authors.lastname: Dowling
authors.forename: A J
authors.initials: AJ
authors.lastname: Dart
authors.forename: R J
authors.initials: RJ
authors.lastname: Rose
authors.forename: D R
authors.initials: DR
authors.lastname: Hodgson
mesh_terms: D000818:Animals; D017364:Collagenases / Q000008:administration & dosage; D005260:Female; D005552:Forelimb / Q000293:injuries; D013006:Growth Hormone / Q000494:pharmacology; D006734:Horse Diseases / Q000000981:diagnostic imaging* / Q000188:drug therapy / Q000473:pathology; D006736:Horses; D007091:Image Processing, Computer-Assisted; D008297:Male; D013708:Tendon Injuries / Q000000981:diagnostic imaging / Q000188:drug therapy / Q000473:pathology / Q000662:veterinary*; D014463:Ultrasonography; D014743:Videotape Recording
chemical_list: D013006:Growth Hormone; D017364:Collagenases