Immunochemical control of the species origin of intestinal mucosa used for heparin purification.
Species specific antisera against bovine, ovine, and porcine serum albumin were produced in order to control the absence of bovine, ovine, or caprine tissues in the porcine intestinal mucosa used for heparin production. Two immunoassays were developed. An enzyme linked immunosorbent assay (ELISA) was very sensitive down to 1 ng/mL bovine albumin or 10 ppm bovine intestinal mucosa in porcine intestinal mucosa. For routine control, a more convenient single radial immunodiffusion assay (SRID) was found suitable to detect 2 microg/mL albumin or 3 p 1000 bovine, ovine, or caprine intestinal mucosa in porcine intestinal mucosa. Conditions of extraction of albumin from intestinal mucosa were optimized and a CV % of 4.1 was obtained for its quantitation. Due to higher albumin concentrations, detection of bovine hashed gut and lung was more sensitive (1.5 and 0.9 p 1000, respectively). Using antisera raised against porcine albumin the SRID can be applied to certify the porcine origin of the intestinal mucosa used for heparin purification and to control its adequate conservation before analysis.
Levieux
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INRA, Meat Research Station, Immunochemistry Unit, Saint-Genès-Champanelle, France.
Levieux
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A
D000418:Albumins / Q000302:isolation & purification; D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D006493:Heparin / Q000302:isolation & purification*; D005779:Immunodiffusion; D007070:Immunoglobulin A / Q000302:isolation & purification; D007074:Immunoglobulin G / Q000302:isolation & purification; D007413:Intestinal Mucosa / Q000378:metabolism*; D012418:Ruminants; D013045:Species Specificity
D000418:Albumins; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D006493:Heparin