title: Solid human embryonic spinal cord xenografts in acute and chronic spinal cord cavities: a morphological and functional study.
abstract: While therapeutic spinal cord grafting procedures are of interest in the chronic spinal cord injury stage, previous experimental grafting studies, including human spinal cord tissue, have mainly focused on the acute stage. Therefore, solid human embryonic spinal cord grafts were implanted in acute or chronic spinal cord aspiration cavities of immunodeficient rats to compare the morphological and locomotor outcome to that of lesion alone cases. Locomotor function was assessed using the Basso, Beattie, and Bresnahan open-field locomotor rating scale up to 6 months, while the morphological evaluation of graft survival, growth, and integration was performed at 6 weeks or 6 months after implantation. Graft survival was 94% in both lesion models, while graft growth was enhanced in the chronic compared to the acute cavity group. Human specific Thy-1 and neurofilament immunoreactive fibers were observed up to 7 mm into host white matter, while aminergic fibers were observed up to 1 mm into the grafts. Abundant calcitonin gene-related peptide immunoreactive fibers in the grafts in the absence both of immunoreactive cell bodies and colocalized human-specific neurofilament immunoreactivity, suggested host fiber ingrowth. At 6 months, the grafted cases presented less central canal deformation and lower glial fibrillary acidic protein immunoreactivity at the host cavity border compared to that of the nongrafted cases. The strong compensatory regain of locomotor function after unilateral spinal cord lesions was not affected by the human spinal cord grafts. In conclusion, solid human embryonic spinal cord tissue transplanted to a cavity in the adult injured spinal cord results in beneficial morphological effects in both the acute and chronic spinal cord lesion.
authors.affiliation: Department of NEUROTEC, Karolinska Institutet, Huddinge University Hospital, S-141 86, Sweden.
authors.forename: E
authors.initials: E
authors.lastname: Akesson
authors.forename: L
authors.initials: L
authors.lastname: Holmberg
authors.forename: M E
authors.initials: ME
authors.lastname: Jönhagen
authors.forename: A
authors.initials: A
authors.lastname: Kjaeldgaard
authors.forename: S
authors.initials: S
authors.lastname: Falci
authors.forename: E
authors.initials: E
authors.lastname: Sundström
authors.forename: A
authors.initials: A
authors.lastname: Seiger
mesh_terms: D000818:Animals; D004622:Embryo, Mammalian; D005260:Female; D016332:Fetal Tissue Transplantation / Q000379:methods / Q000473:pathology / Q000502:physiology*; D005865:Gestational Age; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D005911:Gliosis; D006801:Humans; D007797:Laminin / Q000032:analysis; D009043:Motor Activity / Q000502:physiology*; D051381:Rats; D011923:Rats, Nude; D013116:Spinal Cord / Q000166:cytology / Q000502:physiology / Q000637:transplantation*; D013119:Spinal Cord Injuries / Q000473:pathology / Q000503:physiopathology / Q000601:surgery*; D014183:Transplantation, Heterologous / Q000379:methods / Q000473:pathology / Q000502:physiology*
chemical_list: D005904:Glial Fibrillary Acidic Protein; D007797:Laminin