Antimicrobial resistance among Pseudomonas spp. and the Bacillus cereus group isolated from Danish agricultural soil.
From four Danish pig farms, bacteria of Pseudomonas spp. and the Bacillus cereus group were isolated from soil and susceptibility towards selected antimicrobials was tested. From each farm, soil samples representing soil just before and after spread of animal waste and undisturbed agricultural soil, when possible, were collected. Soil from a well-characterized Danish farm soil (Højbakkegaard) was collected for comparison. The Pseudomonas spp. and B. cereus were chosen as representative for Gram-negative and Gram-positive indigenous soil bacteria to test the effect of spread of animal waste on selection of resistance among soil bacteria. No variations in resistance levels were observed between farms; but when the four differently treated soils were compared, resistance was seen for carbadox, chloramphenicol, nalidixan (nalidixic acid), nitrofurantoin, streptomycin and tetracycline for Pseudomonas spp., and for bacitracin, erythromycin, penicillin and streptomycin for the B. cereus group. Variations in resistance levels were observed when soil before and after spread of animal waste was compared, indicating an effect from spread of animal waste.
Jensen
L B
LB
Danish Veterinary Laboratory, Copenhagen. lje@svs.dk
Baloda
S
S
Boye
M
M
Aarestrup
F M
FM
D000383:Agriculture; D000818:Animals; D001409:Bacillus cereus / Q000187:drug effects* / Q000502:physiology*; D003247:Conservation of Natural Resources; D004351:Drug Resistance; D008372:Manure / Q000382:microbiology*; D008826:Microbial Sensitivity Tests; D011549:Pseudomonas / Q000187:drug effects* / Q000502:physiology*; D012988:Soil Microbiology; D013552:Swine
D008372:Manure