The restriction-modification system in Streptomyces flavopersicus.
To clone bifunctional vectors in streptomycetes, it was necessary to define the restriction-modification system of Streptomyces flavopersicus. Plasmid DNA from bifunctional vectors pIJ699 and pXED3-13, isolated from E. coli strains with different methylation systems: E. coli DH5 alpha (dam+ dcm+), E. coli MB5386 (dam dcm), E. coli CB51 (dam dcm+), E. coli NM544 (dam+ dcm), was used for transformation of protoplasts from strain S. flavopersicus. Restriction of dcm-methylated DNA from S. flavopersicus was established. As a host in the intermediate cloning strain E. coli NM544 (dam+ dcm) should be used, as the dcm-transmethylase-dependent strain S. flavopersicus does not process DNA from this strain.
Lyutzkanova
D
D
Institute of Microbiology, Bulgarian Academy of Sciences, Sofia 1113, Bulgaria.
Stoilova-Disheva
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M
Peltekova
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V
D003001:Cloning, Molecular; D019175:DNA Methylation; D015280:DNA Restriction-Modification Enzymes / Q000378:metabolism*; D004274:DNA, Recombinant / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D010957:Plasmids; D013302:Streptomyces / Q000201:enzymology* / Q000235:genetics; D014170:Transformation, Genetic
D015280:DNA Restriction-Modification Enzymes; D004274:DNA, Recombinant