title: Reinnervation of the superior colliculus delays down-regulation of ephrin A2 in neonatal rat.
abstract: Although the adult mammalian optic nerve does not regenerate following lesion, in the neonatal rat, retinal ganglion cell (RGC) axons retain the capacity to grow across lesion sites in the brain. Following a brachial lesion at postnatal day 2 (P2), some RGC axons, together with ingrowing cortico-tectal axons, cross the lesion to reinnervate the superior colliculus (SC). Here we use immunohistochemistry to examine expression of the guidance cue ephrin A2 following a brachial lesion. Normal animals show a steady decrease in ephrin A2 immunoreactivity between P5 and P31, with a low rostral to high caudal gradient being evident only at P5. By contrast, after brachial lesion, values are significantly elevated rostrally at P5 and caudally at P12; moreover, a steep rostro-caudal gradient is present at both ages. By P31 values fall to normal levels. Following unilateral enucleation at P2, levels are not significantly different from normal. Our results show that innervation but not denervation triggers increased ephrin A2 expression after a brachial lesion.
authors.affiliation: Department of Zoology, University of Western Australia, Crawley, Western Australia, 6009, Australia.
authors.forename: A C
authors.initials: AC
authors.lastname: Symonds
authors.forename: J
authors.initials: J
authors.lastname: Rodger
authors.forename: M M
authors.initials: MM
authors.lastname: Tan
authors.forename: S A
authors.initials: SA
authors.lastname: Dunlop
authors.forename: L D
authors.initials: LD
authors.lastname: Beazley
authors.forename: A R
authors.initials: AR
authors.lastname: Harvey
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001369:Axons / Q000502:physiology; D001930:Brain Injuries / Q000473:pathology / Q000503:physiopathology*; D036383:Ephrin-A2; D015353:Eye Enucleation; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D007150:Immunohistochemistry; D009416:Nerve Regeneration / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D012165:Retinal Ganglion Cells / Q000166:cytology / Q000502:physiology*; D013477:Superior Colliculi / Q000473:pathology / Q000502:physiology* / Q000503:physiopathology; D014157:Transcription Factors / Q000032:analysis* / Q000235:genetics; D014795:Visual Pathways / Q000473:pathology / Q000502:physiology / Q000503:physiopathology
chemical_list: D036383:Ephrin-A2; D014157:Transcription Factors