Determination of mephenoxalone in human plasma sample by high-performance liquid chromatography-fluorescence detection.
A simple and sensitive high-performance liquid chromatographic method involving fluorescence detection was developed for the determination of mephenoxalone in human plasma. A Cosmosil 5C18-MS column (250 mm x 4.6 mm I.D., 5 microm) was used as stationary phase and the mobile phase consisted of water-acetic acid-acetonitrile (200:1:300) at a flow-rate of 1.0 ml/min. The fluorescence absorbance was monitored at 280 nm for excitation wavelength and 310 nm for emission wavelength. Temperature control was kept at 40 degrees C for the column. The limit of quantitation achieved was 10 ng/ml, and the standard curve was found to be linear in the concentration range of 10-10,000 ng/ml. Under these analytical conditions, a sufficient mephenoxalone plasma concentration profile could be obtained for pharmacokinetic study.
Uang
Y S
YS
Protech Pharmaservices Corporation, Taipei, Taiwan.
Chen
I K
IK
Wang
L H
LH
Hsu
K Y
KY
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D009125:Muscle Relaxants, Central / Q000097:blood* / Q000493:pharmacokinetics; D010080:Oxazoles / Q000097:blood* / Q000493:pharmacokinetics; D012016:Reference Values; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*
D009125:Muscle Relaxants, Central; D010080:Oxazoles; C100282:mephenoxalone