HSR203 antisense suppression in tobacco accelerates development of hypersensitive cell death.
Activation of the tobacco gene hsr203 is rapid, highly localized, specific for incompatible plant-pathogen interactions, and strongly correlated with programmed cell death occurring in response to diverse pathogens. Functional characterization of hsr203 gene product has shown that HSR203 is a serine hydrolase that displays esterase activity. We show here that transgenic tobacco plants deficient in HSR203 protein exhibit an accelerated hypersensitive response when inoculated with an avirulent strain of Ralstonia solanacearum. This response was accompanied by a maximal level of cell death and a drastic inhibition of in planta bacterial growth. Transgenic plants deficient in HSR203 were also found to show increased resistance in a dosage-dependent manner to Pseudomonas syringae pv. pisi, another avirulent bacterial pathogen, and to virulent and avirulent races of Phytophthora parasitica, a fungal pathogen of tobacco, but not to different virulent bacteria. Surprisingly, expression of another hsr gene, hsr515, and that of the defence genes PR1-a and PR5, was strongly reduced in the transgenic lines. Our results suggest that hsr203 antisense suppression in tobacco can have pleiotropic effects on HR cell death and defence mechanisms, and induces increased resistance to different pathogens.
Tronchet
M
M
Laboratoire de Biologie Moléculaire des Relations Plantes-Microorganismes, UMR CNRS/INRA 215, BP 27, F-31326 Castanet-Tolosan cedex, France.
Ranty
B
B
Marco
Y
Y
Roby
D
D
D002455:Cell Division / Q000235:genetics*; D004950:Esterases / Q000235:genetics*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D016946:Gram-Negative Aerobic Rods and Cocci / Q000472:pathogenicity; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D010838:Phytophthora / Q000472:pathogenicity; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000166:cytology / Q000235:genetics / Q000382:microbiology; D010947:Plants, Toxic; D011549:Pseudomonas / Q000472:pathogenicity; D014026:Nicotiana / Q000166:cytology / Q000235:genetics* / Q000382:microbiology
D016376:Oligonucleotides, Antisense; D010940:Plant Proteins; D004950:Esterases; C088007:hsr203J protein, Nicotiana tabacum