Determination of the neutralizing potency of horse antibothropic and anticrotalic antivenoms in blood samples collected on filter paper.
The correlation coefficients between in vivo neutralization of lethal toxicity (ED(50)) and levels of antibodies measured by enzyme-linked immunosorbent assay (ELISA) in blood samples collected on filter paper were investigated to test the potency of horse antibothropic and anticrotalic antivenoms. Sixteen horses were hyperimmunized with Bothrops venom (50% from B. jararaca and 12.5% each from B. alternatus, B. jararacussu, B. neuwiedii and B. moojeni) and 12 horses with Crotalus durissus terrificus venom. Crude venom of C. d. terrificus and the lethal fraction of B. jararaca venom were used as antigens to set up an indirect ELISA. The correlation coefficient between ED(50) and ELISA antibodies titers against C. d. terrificus and the lethal fraction of B. jararaca venom was r = 0.8 (P<0.001) and r = 0.78 (P<0.001), respectively.
Maria
W S
WS
Fundação Ezequiel Dias (FUNED), Rua Conde Pereira Carneiro 80, Gameleira, 30510-010, Belo Horizonte, MG, Brazil.
Pacheco
B G
BG
Barbosa
C F
CF
Velarde
D T
DT
Chávez-Olórtegui
C
C
D000818:Animals; D000997:Antivenins / Q000276:immunology* / Q000494:pharmacology; D001800:Blood Specimen Collection / Q000379:methods*; D017837:Bothrops / Q000276:immunology*; D003435:Crotalid Venoms / Q000276:immunology* / Q000633:toxicity; D017839:Crotalus / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006736:Horses / Q000097:blood / Q000276:immunology*; D007114:Immunization; D066298:In Vitro Techniques; D007274:Injections, Intraperitoneal; D051379:Mice; D009500:Neutralization Tests
D000997:Antivenins; D003435:Crotalid Venoms