title: Cutting edge: selective usage of chemokine receptors by plasmacytoid dendritic cells.
abstract: The existence of dendritic cell (DC) subsets is firmly established, but their trafficking properties are virtually unknown. In this study, we show that myeloid (M-DCs) and plasmacytoid (P-DCs) DCs isolated from human blood differ widely in the capacity to migrate to chemotactic stimuli. The pattern of chemokine receptors expressed by blood M-DCs and P-DCs, with the exception of CCR7, is similar. However, most chemokine receptors of P-DCs, in particular those specific for inflammatory chemokines and classical chemotactic agonists, are not functional in circulating cells. Following maturation induced by CD40 ligation, the receptors for inflammatory chemokines are down-regulated, and CCR7 on P-DCs becomes coupled to migration. The drastically impaired capacity of blood P-DCs to migrate in response to inflammatory chemotactic signals contrasts with the response to lymph node-homing chemokines, indicating a propensity to migrate to secondary lymphoid organs rather than to sites of inflammation.
authors.affiliation: Roche Milano Ricerche, Milan, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Penna
authors.forename: S
authors.initials: S
authors.lastname: Sozzani
authors.forename: L
authors.initials: L
authors.lastname: Adorini
mesh_terms: D002454:Cell Differentiation / Q000276:immunology; D002634:Chemotaxis, Leukocyte / Q000276:immunology; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology* / Q000378:metabolism*; D005434:Flow Cytometry; D006801:Humans; D022423:Myeloid Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D010950:Plasma Cells / Q000166:cytology / Q000276:immunology* / Q000378:metabolism*; D019707:Receptors, Chemokine / Q000096:biosynthesis / Q000502:physiology*
chemical_list: D019707:Receptors, Chemokine