[Cloning and expression of TTV gene fragment in E. coil and identification of the expressed protein].
OBJECTIVE
To construct a recombinant expression vector containing TTV gene fragment and identify the expressed protein.


METHODS
Based on computer-aided studies, TTV ORF12 gene fragment coding protein of antigenicity was amplified by PCR from the patient serum with non-A-G hepatitis. Then the gene fragment was integrated into the multiple cloning site of pQE-30 and expressed in E coil. The expressed protein was identified by ELISA.


RESULTS
The relative molecular weight of the expressed protein matches the predication and its yield was up to 22.33% of total bacterial proteins of E. coli The expressed protein was detected by ELISA.


CONCLUSIONS
The recombinant vector has been constructed successfully and the expressed protein possesses antigenicity.
Li
B
B
Department of Immunology, 302 Hospital of PLA, Beijing 100039, China.
Cheng
Y
Y
Hou
J
J
D000293:Adolescent; D000328:Adult; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005260:Female; D015870:Gene Expression; D005814:Genes, Viral / Q000235:genetics*; D006525:Hepatitis, Viral, Human / Q000821:virology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D022783:Torque teno virus / Q000235:genetics* / Q000302:isolation & purification; D014764:Viral Proteins / Q000378:metabolism*
D014764:Viral Proteins