[An animal model for the mucosal immunization with the surface protein antigen P1 from streptococcus mutans and immune enhancement of cholera toxin].
To study the immune enhancement of cholera toxin by developing an mucosal immune animal model, SD rats were immunized using different antigen composition through intragastric route (I.G). The results showed that the effects of immunization became stronger after P1 was conjugated to cholera toxin B subunit (CTB) or procholeragenoid (PCG). Even microgram dose of P1 could induce the rising of anti-P1 SIgA antibodies in the rat's saliva, but the antibodies risied little without this adjuvant. These implayed that the conjugation was much necessary in IG immunization. The immune enhancement of PCG was greater than that of CTB.
Li
F
F
College of Stomatology, West China University of Medical Sciences.
Luo
Z
Z
Zhang
J
J
D000818:Animals; D000942:Antigens, Bacterial / Q000302:isolation & purification / Q000494:pharmacology; D001426:Bacterial Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D002772:Cholera Toxin / Q000494:pharmacology*; D005260:Female; D007071:Immunoglobulin A, Secretory / Q000378:metabolism*; D000922:Immunotoxins / Q000494:pharmacology*; D008297:Male; D008562:Membrane Glycoproteins; D023421:Models, Animal; D010446:Peptide Fragments / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012463:Saliva / Q000378:metabolism*
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D007071:Immunoglobulin A, Secretory; D000922:Immunotoxins; D008562:Membrane Glycoproteins; D010446:Peptide Fragments; C055764:S-layer proteins; C050310:cholera toxin, B subunit (50-64); D002772:Cholera Toxin