[Interactions between ciprofloxacin, Mg2+, Mn2+ and DNA by electrochemical method].
Interactions between ciprofloxacin (CPFX), Mg2+, Mn2+ and DNA, and their polarographic and voltammetric behaviour were studied. In 0.1 mol.L-1 NH3-NH4Cl buffer solution (pH 9.2), a new reduction peak was obtained by linear-sweep voltammetry with Ep = -1.72 V(vs Ag/AgCl) when adding DNA to CPFX solution, which implies binding of CPFX with DNA. In the presence of Mg2+ or Mn2+, another new sensitive reduction peak, whose peak potential is more negative (Ep = -1.78 V), was obtained which suggested that Mg2+ or Mn2+ took part in the interaction between CPFX and DNA resulting in a ternary complex of CPFX-Mg-DNA. The peak current (ip) is proportional to the concentration of DNA over the range of 1.18 x 10(-4)-3.33 x 10(-4) mol.L-1. In this paper, the properties of the peak current were studied in detail, the result showed that the electrode reaction was irreversible and the ip was influenced by adsorption. The electrode reaction mechanism was also probed into. The CPFX molecule in the complex was reduced on the electrode. Furthermore, CPFX-Mg was shown ot be intercalated between the stacked base pairs of native DNA.
Zhang
J L
JL
Department of Chemistry, Shanxi University, Taiyuan 030006.
Fan
H Z
HZ
Pan
J H
JH
D002939:Ciprofloxacin / Q000494:pharmacology*; D004247:DNA / Q000494:pharmacology; D004347:Drug Interactions; D004563:Electrochemistry; D008274:Magnesium / Q000494:pharmacology*; D008345:Manganese / Q000494:pharmacology*
D008345:Manganese; D002939:Ciprofloxacin; D004247:DNA; D008274:Magnesium