Monoclonal antibodies to intra-acrosomal proteins inhibit gamete binding in vitro.
The sperm-zona pellucida-binding assay in vitro was used as a functional test for zona pellucida-binding ability of boar spermatozoa after co-incubation with monoclonal antibodies against intra-acrosomal proteins. The effect of monoclonal antibodies ACR.2 against boar acrosin (55, 53, 45 and 38 kDa), and Hs-8 against boar intra-acrosomal protein (230, 110, 88, 60, 48 kDa) on boar spermatozoa-porcine oocyte binding was examined. The sperm-zona pellucida-binding was reduced when medium was supplemented with monoclonal antibodies during sperm-oocyte co-incubation, but not when capacitated spermatozoa were pretreated with monoclonal antibodies before incubation with oocytes. Our results show that the monoclonal antibodies (ACR.2, Hs-8) against intra-acrosomal proteins reduce the secondary sperm-zona pellucida-binding with statistically significant difference. This suggests the role of these proteins in the early phases of fertilization.
Peknicova
J
J
Department of Biology and Biochemistry of Fertilization, Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Prague. jpeknic@biomed.cas.cz
Capkova
J
J
Geussova
G
G
Ivanova
M
M
Mollova
M
M
D000176:Acrosin / Q000276:immunology; D000177:Acrosome / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D005260:Female; D066298:In Vitro Techniques; D008297:Male; D013084:Sperm-Ovum Interactions / Q000276:immunology*; D013552:Swine
D000911:Antibodies, Monoclonal; D000176:Acrosin