[mRNA expression of metastasis-suppressor gene nm23 in carcinoma of buccal mucosa. II. Quantitative reverse transcription PCR amplification].
The nm23 gene is a conspicuous metastasis-suppressor gene. The authors detected both nm23-H1 and nm23-H2 mRNA levels in 47 tissues samples of patients with carcinoma of buccal mucosa (CBM) by quantitative reverse transcription PCR amplification. The results showed that expression levels of both nm23-H1 and nm23-H2 mRNA varied in normal buccal mucosa, leukoplakia, adjacent nontumorous mucosa, submandibular gland, CBM and lymph nodes with or without metastasis. The nm23-H1 mRNA expression levels in CBM with lymph nodes metastases were lower than those in CBM without metastases (P < 0.05), while no significance of nm23-H2 mRNA expression levels was found between CBM with and CBM without metastasis (P > 0.05). The results were comparative to those of Northern blotting of the same cases. The authors concluded that, as also in the study of Northern blotting, the expression of nm23-H1 mRNA significantly correlated inversely with lymph node metastasis in CBM, while the expression of nm23-H2 mRNA not. Q-RT-PCR was a useful method to detect the mRNA levels of nm23 gene in buccal carcinoma.
Gong
L
L
Department of Oral Pathology, College of Stomatology, West China University of Medical Sciences, China.
He
Z
Z
Lu
Y
Y
D000951:Antigens, Neoplasm / Q000096:biosynthesis; D014408:Biomarkers, Tumor; D015870:Gene Expression; D016147:Genes, Tumor Suppressor; D006801:Humans; D007972:Leukoplakia, Oral / Q000235:genetics*; D008198:Lymph Nodes / Q000378:metabolism; D008207:Lymphatic Metastasis; D020559:Monomeric GTP-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics; D009061:Mouth Mucosa / Q000378:metabolism; D009062:Mouth Neoplasms / Q000235:genetics* / Q000378:metabolism; D054778:NM23 Nucleoside Diphosphate Kinases; D009701:Nucleoside-Diphosphate Kinase; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics
D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D054778:NM23 Nucleoside Diphosphate Kinases; D012333:RNA, Messenger; D014157:Transcription Factors; C517938:NME1 protein, human; D009701:Nucleoside-Diphosphate Kinase; D020559:Monomeric GTP-Binding Proteins