[Relationship between methylenetetrahydrofolate reductase gene polymorphism and diabetic nephropathy].
OBJECTIVE
To investigate the relationship between methylenetetrahydrofolate reductase(MTHFR) gene polymorphism and diabetic nephropathy(DN).


METHODS
The technology of polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) was used to detect MTHFR gene C677T mutation in 85 healthy controls, 79 type-2 diabetics without DN, and 82 type-2 diabetics with DN confirmed by urine microalbuminous detection.


RESULTS
The frequencies of MTHFR gene variant genotypes and alleles in the patients with DN patients were significantly higher than those in the patients without DN and those in healthy controls (P<0.01).


CONCLUSIONS
MTHFR gene C677T mutation is probably one of the genetic risk factors for diabetic nephropathy in Chinese Hans.
Wang
L
L
Department of Endocrinology, No.187 Hospital of PLA, Haikou, Hainan 571159 P.R.China. qinglong@hk.hi.cn
Wang
J
J
Xue
Y
Y
Chen
Y
Y
Zou
H
H
D044466:Asian People / Q000235:genetics; D003928:Diabetic Nephropathies / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006710:Homocysteine / Q000235:genetics; D006801:Humans; D008297:Male; D042965:Methylenetetrahydrofolate Reductase (NADPH2) / Q000235:genetics*; D008875:Middle Aged; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length
D006710:Homocysteine; D042965:Methylenetetrahydrofolate Reductase (NADPH2)