title: A highly polymorphic degenerate microsatellite for molecular strain typing of Candida krusei.
abstract: Simple sequence repeats, due to their high variability, are widely used for molecular epidemiology of pathogenic micro-organisms. However, their usefulness is restricted by their high instability and low information content. Here, a locus, CKTNR, in the fungal pathogen Candida krusei is described which displays considerable sequence, as well as length, heterogeneity. Alleles of this locus, which contains a degenerate trinucleotide repeat, appear to be stable. The CKTNR polymorphism could serve as the basis for a molecular typing system of C. krusei. Furthermore, analysis of the CKTNR allele distribution suggested that C. krusei reproduces mainly clonally.
authors.affiliation: Department of Molecular Microbiology, B. Rappaport Faculty of Medicine1, and Department of Clinical Microbiology2, Rambam Medical Center, Haifa 31096, Israel.
authors.forename: Revital
authors.initials: R
authors.lastname: Shemer
authors.affiliation: Department of Molecular Microbiology, B. Rappaport Faculty of Medicine1, and Department of Clinical Microbiology2, Rambam Medical Center, Haifa 31096, Israel.
authors.forename: Ziva
authors.initials: Z
authors.lastname: Weissman
authors.affiliation: Department of Molecular Microbiology, B. Rappaport Faculty of Medicine1, and Department of Clinical Microbiology2, Rambam Medical Center, Haifa 31096, Israel.
authors.forename: Nehama
authors.initials: N
authors.lastname: Hashman
authors.affiliation: Department of Molecular Microbiology, B. Rappaport Faculty of Medicine1, and Department of Clinical Microbiology2, Rambam Medical Center, Haifa 31096, Israel.
authors.forename: Daniel
authors.initials: D
authors.lastname: Kornitzer
mesh_terms: D002175:Candida / Q000145:classification* / Q000235:genetics*; D002177:Candidiasis / Q000382:microbiology*; D006239:Haplotypes; D006801:Humans; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic; D018911:Trinucleotide Repeats / Q000235:genetics*