title: Vdelta1 T lymphocytes expressing a Th1 phenotype are the major gammadelta T cell subset infiltrating the liver of HCV-infected persons.
abstract: BACKGROUND
  Hepatitis C infection induces an acute and chronic liver inflammation that may lead to cirrhosis, liver failure, or hepatocarcinoma. Since the role of alphabeta T lymphocytes in hepatitis C virus (HCV) immunopathology has been analyzed extensively, we investigated the distribution and functional activation of gammadelta T cell subsets in chronically HCV-infected patients.
  
  
  METHODS
  Blood samples and liver biopsies from 35 patients with compensated chronic HCV infection were compared in terms of T cell subset distribution, expression of activation markers, gammadelta T cell receptor (TCR) repertoire, and pattern of cytokine production. Moreover, we analyzed whether these immunological parameters were associated with other clinical observations (plasma viremia, ALT levels, Ishak index).
  
  
  RESULTS
  Differing from peripheral blood distribution, a specific compartmentalization of Vdelta1 T cells (p < 0.001) was observed in the liver of HCV patients. These cells represented a relevant fraction of intrahepatic T lymphocytes (1.8-8.7%) and expressed the memory/effector phenotype (CD62-L- CD45-RO+CD95+). This phenotype was consistent with selective homing upon antigen recognition. Mitogenic stimulation of Vdelta1 + T lymphocytes recruited in the liver revealed the T helper cell type 1 (Th1) pattern of cytokine secretion. Interestingly, the frequency of interferon-gamma (IFN-gamma)-producing Vdelta1 T cells was associated with an higher degree of liver necroinflammation, measured by the Ishak index. Finally, the T-cell repertoire analysis revealed the absence of Vgamma selection in the TCR repertoire of intrahepatic Vdelta1 T cells.
  
  
  CONCLUSIONS
  gammadelta T cell distribution in the peripheral blood differs from the Vdelta1 T cell subset because it is policlonally activated and recruited in the liver of chronic HCV-infected patients. During HCV-infection, this T cell subset may release Th1 cytokines and contribute to the necroinflammatory liver disease.
authors.affiliation: International Centre for AIDS and Emerging Infections, National Institute for Infectious Diseases (IRCCS), Rome, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Agrati
authors.forename: G
authors.initials: G
authors.lastname: D'Offizi
authors.forename: P
authors.initials: P
authors.lastname: Narciso
authors.forename: S
authors.initials: S
authors.lastname: Abrignani
authors.forename: G
authors.initials: G
authors.lastname: Ippolito
authors.forename: V
authors.initials: V
authors.lastname: Colizzi
authors.forename: F
authors.initials: F
authors.lastname: Poccia
mesh_terms: D000328:Adult; D018414:CD8-Positive T-Lymphocytes; D016207:Cytokines / Q000378:metabolism; D005260:Female; D006526:Hepatitis C / Q000276:immunology / Q000473:pathology*; D006801:Humans; D019041:L-Selectin; D017493:Leukocyte Common Antigens; D008099:Liver / Q000276:immunology / Q000473:pathology* / Q000821:virology; D008297:Male; D008875:Middle Aged; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000378:metabolism*; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D018417:Th1 Cells / Q000276:immunology / Q000378:metabolism*
chemical_list: D016207:Cytokines; D016692:Receptors, Antigen, T-Cell, gamma-delta; D019041:L-Selectin; D017493:Leukocyte Common Antigens; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1