title: IgG-mediated histamine release from canine mastocytoma-derived cells.
abstract: BACKGROUND
  Recent data suggest that normal tissue mast cells can express functional receptors for IgG under certain conditions. However, little is known about IgG receptor expression and functional consequences in mast cell neoplasms.
  
  
  METHODS
  In this study, neoplastic mast cells were obtained from a dog with cutaneous mastocytoma (CM-MC) and from a dog with visceral mastocytoma (VI-MC). Both cell populations were characterized morphologically and functionally.
  
  
  RESULTS
  Most cells proliferated constantly in suspension without particular supplements. Doubling times of CM-MC and VI-MC were 52.2 and 27.5 h, respectively. Both cell types were sensitive to formalin fixation, did not contain heparin and were tryptase and chymase positive. Electron microscopy showed fine granules with electron-dense content in both cell populations. The total histamine content of CM-MC and VI-MC was 0.25 and 0.10 pg/cell, respectively. Calcium ionophore A23187 and substance P induced dose-dependent histamine release, whereas compound 48/80 had no effect. Most significantly, both cell types, when sensitized with monomeric dog IgG, released histamine upon stimulation by anti-dog IgG.
  
  
  CONCLUSIONS
  Dog mastocytoma-derived cells may be useful to study the regulation of neoplastic mast cell growth and differentiation, as well as IgG receptor-mediated activation in neoplastic mast cells. Further research is required to clarify the pathophysiological significance of constitutive expression of IgG receptors in neoplastic (canine) mast cells.
authors.affiliation: Laboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Takahashi
authors.forename: S
authors.initials: S
authors.lastname: Kitani
authors.forename: M
authors.initials: M
authors.lastname: Nagase
authors.forename: M
authors.initials: M
authors.lastname: Mochizuki
authors.forename: R
authors.initials: R
authors.lastname: Nishimura
authors.forename: Y
authors.initials: Y
authors.lastname: Morita
authors.forename: N
authors.initials: N
authors.lastname: Sasaki
mesh_terms: D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002460:Cell Line; D053818:Chymases; D004283:Dog Diseases / Q000276:immunology* / Q000473:pathology; D004285:Dogs / Q000276:immunology*; D005260:Female; D006636:Histamine Release; D007074:Immunoglobulin G / Q000494:pharmacology*; D007414:Intestinal Neoplasms / Q000648:ultrastructure / Q000662:veterinary*; D007476:Ionophores / Q000494:pharmacology; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000276:immunology* / Q000648:ultrastructure; D012515:Mast-Cell Sarcoma / Q000648:ultrastructure / Q000662:veterinary*; D008854:Microscopy, Electron; D012697:Serine Endopeptidases / Q000032:analysis; D012878:Skin Neoplasms / Q000648:ultrastructure / Q000662:veterinary*; D013373:Substance P / Q000494:pharmacology; D053802:Tryptases; D014407:Tumor Cells, Cultured
chemical_list: D007074:Immunoglobulin G; D007476:Ionophores; D013373:Substance P; D000001:Calcimycin; D012697:Serine Endopeptidases; D053818:Chymases; D053802:Tryptases