title: Presence of interleukin-4-producing cells for human bone regeneration after application of guided tissue regeneration membranes.
abstract: To study the process of bone regeneration we examined three samples of periapical regenerative tissue obtained from two patients under a guided tissue regeneration treatment in endodontic surgery by the immunohistochemical and enzyme histochemical methods. The regenerative tissue consisted of a large number of fibroblast-like cells and a small number of mononuclear cells. Fibroblast-like cells stained positively for alkaline phosphatase and osteopontin, whereas mononuclear cells stained positively for CD4. Interleukin-4-producing cells could be detected in adjacent sections. However, interferon-y-producing cells could not be detected. These findings suggest that interleukin-4-producing cells may be one of the elements associated with success in the human bone regeneration process in vivo.
authors.affiliation: Department of Periodontics and Endodontics, Kyushu University, Fukuoka, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Kabashima
authors.forename: K
authors.initials: K
authors.lastname: Nagata
mesh_terms: D000328:Adult; D000469:Alkaline Phosphatase / Q000032:analysis; D016301:Alveolar Bone Loss / Q000000981:diagnostic imaging / Q000601:surgery; D001861:Bone Regeneration / Q000502:physiology; D015704:CD4 Antigens / Q000032:analysis; D015496:CD4-Positive T-Lymphocytes / Q000473:pathology; D004396:Coloring Agents; D005347:Fibroblasts / Q000473:pathology; D016557:Guided Tissue Regeneration, Periodontal / Q000295:instrumentation*; D006801:Humans; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D007371:Interferon-gamma / Q000032:analysis; D015847:Interleukin-4 / Q000032:analysis*; D007963:Leukocytes, Mononuclear / Q000473:pathology; D008297:Male; D008567:Membranes, Artificial; D008875:Middle Aged; D010006:Osteoblasts / Q000473:pathology; D053495:Osteopontin; D010483:Periapical Diseases / Q000000981:diagnostic imaging / Q000601:surgery; D010750:Phosphoproteins / Q000032:analysis; D011859:Radiography; D012795:Sialoglycoproteins / Q000032:analysis
chemical_list: D015704:CD4 Antigens; D004396:Coloring Agents; D008567:Membranes, Artificial; D010750:Phosphoproteins; C506895:SPP1 protein, human; D012795:Sialoglycoproteins; D053495:Osteopontin; D015847:Interleukin-4; D007371:Interferon-gamma; D000469:Alkaline Phosphatase