Effects of trapidil on ATPase, lipid peroxidation, and correlation with ultrastructure in experimental spinal cord injury.
The present study was performed to investigate the effect of trapidil on ischemic damage of cells after spinal cord injury. The injury was produced by extradural clip compression of the exposed spinal cord in rats according to Rivlin and Tator. The ten rats in group 1 were used to determine normal findings without any surgery or medication. On the 15 rats in group 2, only six-level laminectomy was performed to determine the influence of the total laminectomy on the biochemical factors measured and the, light and ultrastructural findings. The 15 rats each in groups 3 and 4 were used as trauma and trapidil (40 mg/kg) treatment groups, respectively. The injury actually produced a significant decrease in Na+-K+/Mg+2 ATPase activity of the injured segments as early as 10 min after trauma. Trapidil attenuated Na+-K+/Mg+2 ATPase inactivation in the traumatized rats for 120 min after treatment (P<0.05) and significantly reduced the malone dialdehyde content below that in the traumatized group at all determined times (P<0.05). Light and electron microscopic findings supported the biochemical results.
Göçer
A I
AI
Department of Neurosurgery, Cukurova University School of Medicine, Balcali/Adana, Turkey. alpgocer@mail.cu.edu.tr
Ildan
F
F
Tuna
M
M
Polat
S
S
Tamer
L
L
Erman
T
T
Kaya
M
M
D000251:Adenosine Triphosphatases / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D004195:Disease Models, Animal; D007796:Laminectomy; D015227:Lipid Peroxidation / Q000187:drug effects* / Q000502:physiology; D008297:Male; D008854:Microscopy, Electron; D051381:Rats; D017208:Rats, Wistar; D013116:Spinal Cord / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure*; D013117:Spinal Cord Compression / Q000378:metabolism*; D013119:Spinal Cord Injuries / Q000378:metabolism*; D020760:Spinal Cord Ischemia / Q000378:metabolism*; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis; D013904:Thoracic Vertebrae / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D014192:Trapidil / Q000378:metabolism / Q000494:pharmacology*; D014665:Vasodilator Agents / Q000378:metabolism / Q000494:pharmacology*
D017392:Thiobarbituric Acid Reactive Substances; D014665:Vasodilator Agents; D000251:Adenosine Triphosphatases; D014192:Trapidil