Non-aqueous capillary electrophoretic separation of enantiomeric amines with (-)-2,3:4,6-di-O-isopropylidene-2-keto-L-gulonic acid as chiral counter ion.
(-)-2,3:4,6-Di-O-isopropylidene-2-keto-L-gulonic acid [(-)-DIKGA] has been introduced as a chiral counter ion in non-aqueous capillary electrophoresis. High enantioresolutions (R(s)> or =3) were obtained for amines, e.g., pronethalol, labetalol and bambuterol. Methanol containing NaOH and (-)-DIKGA was used as the background electrolyte. The counter ion concentration and the nature of the injection medium were found to affect the chiral separation. Covalent coating of the fused-silica capillary reduced the electro-osmotic flow resulting in improved enantioresolutions.
Carlsson
Y
Y
Division of Analytical Pharmaceutical Chemistry, Uppsala University, Biomedical Centre, Sweden.
Hedeland
M
M
Bondesson
U
U
Pettersson
C
C
D000588:Amines / Q000302:isolation & purification*; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D007202:Indicators and Reagents / Q000737:chemistry*; D015203:Reproducibility of Results; D013237:Stereoisomerism; D013400:Sugar Acids / Q000737:chemistry*
C441632:2,3-4,6-di-O-isopropylidene-2-ketogulonic acid; D000588:Amines; D019086:Bridged Bicyclo Compounds, Heterocyclic; D007202:Indicators and Reagents; D013400:Sugar Acids