Proposed phytic acid standard including a method for its analysis.
A method is described that accurately and rapidly quantifies the free and total phosphorous content of a commercially available, purified, phytic acid preparation. This allows its use as a standard for phytic acid determinations in foods. The method involves a wet ashing step followed by phosphorous measurement with a 1-amino-2-naphthol-4-sulfonic acid-molybdate reagent in a microplate reader at 660 nm. The procedure can be performed in 3 h with as little as 50 mg sample.
Brooks
S P
SP
Health Canada, Nutrition Research Division, Banting Research Centre, Ottawa, ON.
Oberleas
D
D
Dawson
B A
BA
Belonje
B
B
Lampi
B J
BJ
D009682:Magnetic Resonance Spectroscopy; D010758:Phosphorus / Q000032:analysis; D010833:Phytic Acid / Q000032:analysis* / Q000592:standards; D012015:Reference Standards
D010758:Phosphorus; D010833:Phytic Acid