Dehalogenation of dichloromethane by dichloromethane dehalogenase/glutathione S-transferase leads to formation of DNA adducts.
Formation of DNA adducts following conversion of dichloromethane by bacterial dichloromethane dehalogenase/glutathione S-transferase was demonstrated. Adducts included dichloromethane carbon and glutathione sulfur atoms. A reaction with DNA occurred preferentially at guanine bases. Increased DNA degradation in a polA mutant of Methylobacterium dichloromethanicum DM4 grown with dichloromethane confirmed the genotoxicity associated with dichloromethane degradation, suggesting an important role of DNA repair in the metabolism of halogenated, DNA-alkylating compounds by bacteria.
Kayser
M F
MF
Institut für Mikrobiologie, ETH Zürich, CH-8092 Zürich, Switzerland.
Vuilleumier
S
S
D018736:DNA Adducts / Q000378:metabolism*; D004260:DNA Repair; D004269:DNA, Bacterial / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism*; D006219:Halogens / Q000378:metabolism*; D008190:Lyases / Q000378:metabolism*; D008752:Methylene Chloride / Q000378:metabolism*; D020580:Methylobacterium / Q000235:genetics / Q000378:metabolism; D013379:Substrate Specificity
D018736:DNA Adducts; D004269:DNA, Bacterial; D006219:Halogens; D008752:Methylene Chloride; D005982:Glutathione Transferase; D008190:Lyases; C045224:dichloromethane dehalogenase