[Study on the origin of influenza A(H1N2) virus HA and NA genes].
OBJECTIVE
To determine the origins of the HA and NA genes of new subtype (H1N2) of influenza A virus.


METHODS
Virus was amplified in embryonated chicken eggs, then virion RNA was transcribed into cDNA by reverse transcriptase, cDNA amplified by PCR, the products of PCR were purified. Afterward, RNA sequence analysis was performed by the dideoxynucleotide chain termination method, using synthetic oligodideoxynucleotide primers. Finally, phylogenetic analysis of the sequencing data was per-formed with MegAlign (Version 1.03) and Editseq (Version 3.69) software.


RESULTS
The homology of amino acid sequences of protein molecules on HAl domains when compared, with A/PR/8/34(H1N1) and A/Guangdong/6/91(H1 N1) viruses was 98.2% and 99.4%, respectively. The homology of amino acid sequences of NA protein molecules between the novel reassortant and H1N1 virus was only 83.4 %, but the homology of those between H1N2 strain and H3N2 virus circulating in men in 1995 was as high as 99.1 %.


CONCLUSIONS
The new reassortant (H1N2) virus HA gene was derived from A/PR/8/34(H1N1) like virus, but its NA gene was derived from H3N2 virus circulating in men in 1995.
Qi
J
J
Institute of Virology, Chinese Academy of Preventive Medicine, Beijing 100052, China.
Wang
M
M
Dong
J
J
D000328:Adult; D001483:Base Sequence; D002648:Child; D005814:Genes, Viral / Q000235:genetics*; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics*; D006801:Humans; D009980:Influenza A virus / Q000235:genetics*; D007251:Influenza, Human / Q000821:virology*; D008297:Male; D008969:Molecular Sequence Data; D009439:Neuraminidase / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
D019267:Hemagglutinin Glycoproteins, Influenza Virus; D012367:RNA, Viral; D009439:Neuraminidase