[Dual color fluorescent in-situ hybridization detects sex chromosome mosaicism in human embryos].
OBJECTIVE
Using dual color fluorescent in-situ hybridization (FISH) to detect sex chromosome mosaicism in human embryos and perform preimplantation gender diagnosis.


METHODS
Three abnormal fertilized embryos and 18 normal fertilized embryos which were not suitable for embryo transfer and cryopreservation were analyzed by dual color fluorescent in-situ hybridization.


RESULTS
Mosaicism analysis was performed on 14 normal fertilized embryos, which had more than two cells. Nine embryos had sex chromosome mosaicism. XO in male and female embryos cells were 12% and 14% respectively.


CONCLUSIONS
Identification of sex by FISH analysis of a single cleavage cell is accurate, and sex chromosome mosaicism will not affect preimplantation gender diagnosis.
Xu
Y
Y
Reproductive Medical Center, First Affiliated Hospital of Sun Yat-sen University of Medical Sciences, Guangzhou 510080.
Zhuang
G
G
Zhou
C
C
D004624:Embryo Transfer; D004622:Embryo, Mammalian / Q000378:metabolism; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D008297:Male; D009030:Mosaicism; D012730:Sex Chromosomes / Q000235:genetics*; D012732:Sex Determination Analysis