title: The occurrence of E. coli O157:H7 in South African water sources intended for direct and indirect human consumption.
abstract: The occurrence of Escherichia coli O157:H7 in selected water samples in South Africa was investigated. The chromogenic Rainbow agar O157 medium designed for the rapid identification of E. coli O157:H7 was used for the detection of these organisms in various river-water samples in the Vaal Barrage Reservoir drainage basin of South Africa. A total of 204 samples were obtained from 15 sites where water was used for direct and indirect human consumption. Samples were filtered through Gelman filter-units and incubated on Rainbow agar O157 which produced different colours according to the bacterial chromogenic properties. Six hundred and sixty-three suspected E. coli O157:H7 colonies, with colours ranging between dark blue, grey and black, were subcultured onto sorbitol-MacConkey agar and screened for different virulence factors specific for E. coli O157:H7 and agglutination with anti-E. coli O157 antiserum. The results indicated that none of the suspected colonies contained all of the virulence factors necessary to classify them as E. coli O157:H7. None of these organisms agglutinated with antisera against E. coli O157. The probability of being infected with E. coli O157:H7 from direct or indirect consumption of these river water sources is therefore low. Some samples did, however, contain enterohaemorrhagic E. coli virulence properties, such as Stx1, Stx2 and enterohaemolysin, which might impose a health risk if ingested.
authors.affiliation: Department of Medical Virology, Institute of Pathology, University of Pretoria, South Africa. emuller@medic.up.ac.za
authors.forename: E E
authors.initials: EE
authors.lastname: Müller
authors.forename: M M
authors.initials: MM
authors.lastname: Ehlers
authors.forename: W O
authors.initials: WO
authors.lastname: Grabow
mesh_terms: D015169:Colony Count, Microbial; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D019453:Escherichia coli O157 / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D029968:Escherichia coli Proteins; D006460:Hemolysin Proteins / Q000032:analysis / Q000235:genetics; D006801:Humans; D011634:Public Health / Q000592:standards; D013019:South Africa; D014871:Water Microbiology / Q000592:standards; D014873:Water Pollutants / Q000592:standards; D014881:Water Supply / Q000032:analysis* / Q000592:standards
chemical_list: D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D006460:Hemolysin Proteins; D014873:Water Pollutants; C066219:enterohemolysin