title: Location of renin-angiotensin system components in the hypoglossal nucleus of the rat.
abstract: The renin-angiotensin system (RAS) in the hypoglossal nuclei of the rat was studied by immunohistochemistry. Antibodies to angiotensin AT(1) receptor (AT1), angiotensinogen (ANG), renin (REN), angiotensin converting enzyme (ACE) and angiotensin II (AII) were used. All the components of the RAS with the exception of renin were detected. Light and electron microscopy revealed the following results: ANG was predominantly found in astrocytes, with small amounts in neuronal dendrites; ACE was found in the cytoplasm of neurons, dendrites and astrocyte processes; AT1 was found in the cytoplasm of neurons and dendrites, but not on the membrane; and AII was found mainly in astrocytes with some located in the dendrites and cytoplasm. Right hypoglossal nerve lesion caused an increase in expression of AT1 in neurons as early as 2 days post-lesion. An increase in expression of ANG in astrocytes was also seen, but at a much later time of 3 weeks post-lesion. For AII, staining occurred in both the neurons and astrocytes in the undamaged hypoglossal nucleus. Nerve lesion caused a disappearance of neuronal stains and an increase in astrocyte stains. There were no changes in ACE staining after nerve lesion. We speculate that ANG and AII are made within the astrocytes, whereas ACE could either be uptaken from blood or de novo synthesized. AT1 may potentially be internal soluble receptors. As to the function of AII in the hypoglossal nucleus, the data do not support AII as a neurotransmitter in the hypoglossal nucleus. It may function as a neuromodulator and also be involved in basic cellular activities, e.g. regulation of transcription factors.
authors.affiliation: Department of Pharmacology, Faculty of Medicine, National University of Singapore, 119260, Singapore, Singapore.
authors.forename: M
authors.initials: M
authors.lastname: Tham
authors.forename: M K
authors.initials: MK
authors.lastname: Sim
authors.forename: F R
authors.initials: FR
authors.lastname: Tang
mesh_terms: D000804:Angiotensin II / Q000032:analysis / Q000276:immunology / Q000378:metabolism; D000808:Angiotensinogen / Q000032:analysis / Q000276:immunology / Q000378:metabolism; D000818:Animals; D000906:Antibodies / Q000276:immunology; D001253:Astrocytes / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure; D001933:Brain Stem / Q000737:chemistry* / Q000166:cytology / Q000648:ultrastructure; D003712:Dendrites / Q000737:chemistry / Q000648:ultrastructure; D003714:Denervation; D007002:Hypoglossal Nerve / Q000737:chemistry* / Q000166:cytology / Q000601:surgery; D007150:Immunohistochemistry; D008297:Male; D016253:Microscopy, Immunoelectron; D009474:Neurons / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure; D007703:Peptidyl-Dipeptidase A / Q000032:analysis / Q000276:immunology; D051381:Rats; D017208:Rats, Wistar; D044140:Receptor, Angiotensin, Type 1; D011945:Receptors, Angiotensin / Q000032:analysis / Q000276:immunology / Q000378:metabolism; D012083:Renin / Q000032:analysis / Q000276:immunology; D012084:Renin-Angiotensin System; D013997:Time Factors
chemical_list: D000906:Antibodies; D044140:Receptor, Angiotensin, Type 1; D011945:Receptors, Angiotensin; D000808:Angiotensinogen; D000804:Angiotensin II; D007703:Peptidyl-Dipeptidase A; D012083:Renin