[Effects of monocarboxylic acid derivatives on cardiac ventricular CFTR Cl- channels in guinea pig].
Using the whole-cell recording technique, the effects of monocarboxylic acid derivatives on cystic fibrosis transmembrane conductance regulator (CFTR) Cl(-)-channel were examined in guinea pig ventricular myocytes. Anthracene-9-carboxylic acid (9-AC) added to the bath solution further enhanced the outward component of isoproterenol-induced currents in a reversible manner, whereas 5-nitro-2-(3-phenylpropylamino) benzoic acid (NPPB) or diphenylamine-2-carboxylic acid (DPC) induced a biphasic effect on the currents. Either NPPB or DPC first produced a transient increase in the outward component of current before ensuing inhibition. Intracellular NPPB was found to potentiate isoproterenol-activated currents. It is concluded that these monocarboxylic acid derivatives have different binding sites in cardiac ventricular myocytes, which might partially account for the varied effects in blocking anion channels.
Zhou
S S
SS
Department of Physiology, Fourth Military Medical University, Xi'an 710032.
Zang
Y M
YM
D000818:Animals; D000873:Anthracenes / Q000494:pharmacology; D002469:Cell Separation; D018118:Chloride Channels / Q000187:drug effects*; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000187:drug effects* / Q000502:physiology; D004594:Electrophysiology; D005260:Female; D006168:Guinea Pigs; D007545:Isoproterenol / Q000494:pharmacology; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D009579:Nitrobenzoates / Q000494:pharmacology*; D012717:Sesquiterpenes / Q000494:pharmacology; D016276:Ventricular Function; D062367:ortho-Aminobenzoates / Q000494:pharmacology
D000873:Anthracenes; D018118:Chloride Channels; D009579:Nitrobenzoates; D012717:Sesquiterpenes; D062367:ortho-Aminobenzoates; D019005:Cystic Fibrosis Transmembrane Conductance Regulator; C058176:5-nitro-2-(3-phenylpropylamino)benzoic acid; C038797:K 76 carboxylic acid; C010030:9-anthroic acid; C001209:fenamic acid; D007545:Isoproterenol