Tethered-bilayer lipid membranes as a support for membrane-active peptides.
An immunosensing device, comprising a lipid membrane incorporating ion channels tethered to the surface of a gold electrode, has been reported [Cornell, Braach-Maksvytis, King, Osman, Raguse, Wieczorek and Pace (1997) Nature (London) 387, 580-583]. The present article describes key steps in the assembly of the device and provides further evidence for its proposed sensing mechanism.
Cornell
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BA
Ambri Pty Ltd, 126 Greville Street, Chatswood, Sydney, NSW 2067, Australia. bruce@ambri.com.au
Krishna
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G
Osman
P D
PD
Pace
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RD
Wieczorek
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L
D003198:Computer Simulation; D004220:Disulfides / Q000737:chemistry; D004553:Electric Conductivity; D006096:Gramicidin; D008051:Lipid Bilayers / Q000737:chemistry*; D008563:Membrane Lipids / Q000737:chemistry; D008958:Models, Molecular; D008968:Molecular Conformation; D010455:Peptides / Q000737:chemistry*; D013329:Structure-Activity Relationship
D004220:Disulfides; D008051:Lipid Bilayers; D008563:Membrane Lipids; D010455:Peptides; D006096:Gramicidin