1,25(OH)2 vitamin D concentration in the plasma of Solanum glaucophyllum intoxicated rabbits.
OBJECTIVE
To test Solanum glaucophyllum calcinotic effects in adult New Zealand White rabbits in relation to cumulative dose and active principle concentration in plasma.


METHODS
An intoxication assay with controls.


METHODS
Rabbits were orally dosed with aqueous extracts of dry leaves of S glaucophyllum for 5, 7 or 9 days. During the experiment, body weight, calcaemia and phosphataemia were measured; retinal blood vessel calibre was observed by ophthalmoscopic examination of the ocular fundus. 1,25(OH)2 vitamin D plasma concentration was determined at the end of the experimental periods. Soft tissue calcium concentration and the presence of calcinotic lesions were studied after euthanasia.


RESULTS
Toxic effects were evident in S glaucophyllum treated groups (loss of body weight, elevation of soft tissue calcium concentration, and presence of calcinotic lesions). Plasma 1,25(OH)2 vitamin D concentrations were negatively correlated with final body weight (r = -0.97; P < or = 0.001), and positively correlated with renal calcium concentration (r = 0.74; P = 0.02). There was also a significant regression of plasma 1,25 (OH)2 vitamin D concentration on the cumulative dose of S glaucophyllum (R2 = 0.87; P < or = 0.001).


CONCLUSIONS
The procedure described here offers a sensitive and practical experimental model for the study of the pathogenesis of enteque seco.
Dallorso
M E
ME
School of Agricultural Sciences, Lomas de Zamora National University, Provincia de Buenos Aires, Republica Argentina. carou@ciudad.com.ar
Gil
S
S
Pawlak
E
E
Lema
F
F
Márquez
A
A
D000818:Animals; D001011:Aorta / Q000473:pathology; D001835:Body Weight / Q000187:drug effects; D002114:Calcinosis / Q000209:etiology / Q000473:pathology / Q000662:veterinary*; D002118:Calcium / Q000097:blood* / Q000378:metabolism; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D007674:Kidney Diseases / Q000209:etiology / Q000473:pathology / Q000662:veterinary*; D008168:Lung / Q000473:pathology; D010936:Plant Extracts / Q000008:administration & dosage / Q000633:toxicity; D018515:Plant Leaves; D010939:Plant Poisoning / Q000097:blood / Q000150:complications / Q000662:veterinary*; D011817:Rabbits; D019657:Solanaceae / Q000633:toxicity*; D013250:Steroid Hydroxylases / Q000097:blood*; D013270:Stomach / Q000473:pathology
D010936:Plant Extracts; D013250:Steroid Hydroxylases; C406322:vitamin D 1-alpha hydroxylase; D002118:Calcium