[Study on the sensitization of acute myeloid leukemia cell to daunorubicin by recombinant human interleukin-3].
OBJECTIVE
To evaluate the effect of recombinant human interleukin-3(rhIL-3) on the sensitization of acute myeloid leukemia(AML) cells to daunorubicin(DNR).


METHODS
AML cells were cultured in a DNR-containing medium supplemented or not with rhIL-3. CFU-AML was assayed by semi-solid culture, proliferation and differentiation of AML cells were assayed by flow cytometry, cytomorphology and benzidine staining. Intracellular DNR concentration was measured by spectrophotofluorometry.


RESULTS
rhIL-3 could enhance the proliferation but not differentiation of AML cells in vitro, and had no effect on DNR intake and exclusion of AML cells while enhancing their sensitivity to DNR.


CONCLUSIONS
rhIL-3 combined with DNR might improve the therapeutic effect of AML.
Wang
J
J
Department of Hematology, Xin Qiao Hospital, Third Military Medical University, Chongqing 630038.
Wang
Q
Q
Chen
X
X
D000293:Adolescent; D000328:Adult; D000903:Antibiotics, Antineoplastic / Q000378:metabolism*; D002455:Cell Division / Q000187:drug effects; D003630:Daunorubicin / Q000378:metabolism*; D019008:Drug Resistance, Neoplasm; D004357:Drug Synergism; D005260:Female; D006801:Humans; D007377:Interleukin-3 / Q000494:pharmacology*; D008297:Male; D008875:Middle Aged; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000378:metabolism / Q000473:pathology*; D011994:Recombinant Proteins / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000903:Antibiotics, Antineoplastic; D007377:Interleukin-3; D011994:Recombinant Proteins; D003630:Daunorubicin