High-performance affinity capture-removal of bacterial pyrogen from solutions.
Synthetic peptide S3delta has high affinity for bacterial endotoxin or lipopolysaccharide (LPS). Under tested conditions of pH 5-9 and 0-0.4 M NaCl, the affinity constant, KD ranged from 2.10(-6) to 2 x 10(-9) M(-1). A novel affinity matrix based on peptide S3delta was developed for removal of LPS from solutions such as: water; buffers with a wide range of ionic strength and pH; medium for cell culture; and protein solutions under optimized conditions. At a starting LPS of approximately 100 EU/ml, a post-purification level below 0.005 EU/ml was achieved.
Ding
J L
JL
Department of Biological Sciences, Faculty of Sciences, National University of Singapore, Singapore. dbsdjl@nus.edu.sg
Zhu
Y
Y
Ho
B
B
D000595:Amino Acid Sequence; D002846:Chromatography, Affinity / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004731:Endotoxins / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D011749:Pyrogens / Q000302:isolation & purification*; D012996:Solutions
D004731:Endotoxins; D011749:Pyrogens; D012996:Solutions