Characterization of Borrelia burgdorferi isolated from erythema migrans lesions: interrelationship of three molecular typing methods.
Genetic diversity among Borrelia burgdorferi isolates recovered from the skin of Lyme disease patients was assessed by ribosomal DNA (rDNA) spacer restriction fragment length polymorphism analysis, genomic restriction site polymorphism analysis, and plasmid content analysis. There was a significant association between the three rDNA spacer types, the six pulsed-field gel types, and plasmid content (P < 0.001). The association between distinct chromosomal and plasmid markers implies a clonal origin for each genotype.
Iyer
R
R
Department of Biochemistry & Molecular Biology, New York Medical College, Valhalla, New York 10595, USA.
Liveris
D
D
Adams
A
A
Nowakowski
J
J
McKenna
D
D
Bittker
S
S
Cooper
D
D
Wormser
G P
GP
Schwartz
I
I
D000328:Adult; D015373:Bacterial Typing Techniques / Q000379:methods*; D015748:Borrelia burgdorferi Group / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D015787:Erythema Chronicum Migrans / Q000382:microbiology*; D006801:Humans; D010957:Plasmids / Q000235:genetics; D012150:Polymorphism, Restriction Fragment Length
D021903:DNA, Ribosomal Spacer