Presence of four stem cell populations in monolayer cultures derived from teratocarcinoma embryoid bodies.
In the present work, flow cytometry techniques together with morphologic studies were used to perform multiparametric analyses in cell cultures derived from CE44 teratocarcinoma embryoid bodies. The intrinsic cell parameters studied by flow cytometry were size (FALS), cytoplasmic complexity (ISS) and autofluorescence, expressed as LIGFL/FALS (green fluorescence intensity on a logarithmic scale/FALS). Our results showed that CE44 teratocarcinoma yields monolayers whose cells show a marked morphological heterogeneity and can be grouped according to flow cytometric criteria into four populations that remain stable throughout the entire time of culture. Moreover, these populations showed a different immunolabelling with the differentiation markers SSEA-1, TROMA-1 and anti-vimentin.
Hilario
E
E
Department of Cellular Biology and Morphological Sciences, School of Medicine and Dentistry, University of the País Vasco, Leioa, Vizcaya 48940, Spain. gcphiroe@lg.ehu.es
Alvarez
A
A
Simón
J
J
García-Sanz
M
M
Lacalle
J
J
Aréchaga
J
J
D000818:Animals; D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D018236:Carcinoma, Embryonal; D002466:Cell Nucleolus / Q000648:ultrastructure; D003593:Cytoplasm / Q000648:ultrastructure; D005260:Female; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D007633:Keratins / Q000032:analysis / Q000276:immunology; D016256:Lewis X Antigen / Q000032:analysis / Q000276:immunology; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008854:Microscopy, Electron; D009368:Neoplasm Transplantation; D013234:Stem Cells / Q000737:chemistry / Q000648:ultrastructure*; D018243:Teratocarcinoma; D014407:Tumor Cells, Cultured; D014746:Vimentin / Q000032:analysis / Q000276:immunology
D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D016256:Lewis X Antigen; D014746:Vimentin; D007633:Keratins