Effects of beta-casomorphins on neuronal survival in culture of embryonic chick dorsal root ganglion neurons.
We studied the effects of beta-casomorphins (beta-CMs, mu-acting opioid peptides from milk protein beta-casein) on survival of primary-cultured chick dorsal root ganglion neurons in the presence of nerve growth factor. Beta-CM-5 and beta-CM-7 had potent neuronal survival-promoting activities. Beta-CM-4 amide (morphiceptin) and des-Tyr'-beta-CM-7 also exhibited the similar promoting effects, although their effects were very weak. The promoting effect of beta-CM-5 was prevented by co-administration of naloxone, or pretreatment with pertussis toxin. These results suggest that the neuronal survival-promoting effects of beta-CMs might be mediated through opioid receptors coupled to G proteins.
Sakaguchi
M
M
Laboratory of Cell Biology, Osaka University of Pharmaceutical Sciences, Takatsuki, Japan.
Fujimori
T
T
Satoh
T
T
Matsumura
E
E
D000595:Amino Acid Sequence; D000818:Animals; D002470:Cell Survival / Q000187:drug effects*; D002478:Cells, Cultured; D002642:Chick Embryo; D004723:Endorphins / Q000494:pharmacology*; D005727:Ganglia, Spinal / Q000166:cytology / Q000187:drug effects*; D009474:Neurons / Q000166:cytology / Q000187:drug effects*
D004723:Endorphins; C021968:beta-casomorphins