[An experimental study of rat infected with human cytomegalovirus AD169 strain].
OBJECTIVE
To study the sensitivity of rat to human cytomegalovirus (HCMV) infection and to establish a stable animal model.


METHODS
Two kinds of rat experiments were carried out: 1) 30 SD rats were divided into three groups randomly, they were virus inoculated group, inactivated virus inoculated group and the normal rat group respectively; 2) 40 Wistar rats were randomly divided into two groups, they were the virus inoculated group and the inactivated virus inoculated group. All the rats were inoculated through tail vein. 90 d later, the rat's tissue lesions were observed by pathological techniques, HCMV antigen was detected by immunohistochemical method and the HCMV DNA was analyzed by in situ hybridization.


RESULTS
The extensive pathological damages in tissues of HCMV infected rats were observed. Meanwhile, the viral antigen and viral DNA were also demonstrated in many tissues of HCMV infected rats.


CONCLUSIONS
Rat is sensitive to infection of HCMV strain, but, as an animal model study, we still have a lot of works to do.
Meng
H
H
Department of Microbiology, Institute of Basic Medicine, Shandong Acdemy of Medical Sciences, Jinan 250062, China.
Sun
D
D
Wang
J
J
D000818:Animals; D003587:Cytomegalovirus / Q000472:pathogenicity; D003586:Cytomegalovirus Infections / Q000473:pathology; D004195:Disease Models, Animal; D005260:Female; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley