A novel grass pollen allergen mimotope identified by phage display peptide library inhibits allergen-human IgE antibody interaction.
The aim of this study was to investigate the molecular basis of human IgE-allergen interaction by screening a phage-displayed peptide library with an allergen-specific human IgE-mimicking monoclonal antibody (mAb). A mAb that reacted with major grass pollen allergens was successfully identified and shown to inhibit human IgE-allergen interaction. Biopanning of a phage-displayed random peptide library with this mAb yielded a 12 amino acid long mimotope. A synthetic peptide based on this 12-mer mimotope inhibited mAb and human IgE binding to grass pollen extracts. Our results indicate that such synthetic peptide mimotopes of allergens have potential as novel therapeutic agents.
Suphioglu
C
C
Department of Allergy, Asthma and Clinical Immunology, Monash University Medical School, Commercial Road, Prahran, Vic. 3181, Australia. cenk.suphioglu@med.monash.edu.au
Schäppi
G
G
Kenrick
J
J
Levy
D
D
Davies
J M
JM
O'Hehir
R E
RE
D000485:Allergens / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000235:genetics / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D018985:Epitopes, B-Lymphocyte / Q000276:immunology; D006801:Humans; D015151:Immunoblotting / Q000379:methods; D007073:Immunoglobulin E / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D019151:Peptide Library; D010455:Peptides / Q000276:immunology; D006109:Poaceae / Q000276:immunology*; D011058:Pollen / Q000276:immunology*; D017421:Sequence Analysis
D000485:Allergens; D000911:Antibodies, Monoclonal; D018985:Epitopes, B-Lymphocyte; D019151:Peptide Library; D010455:Peptides; D007073:Immunoglobulin E