Combinatorial approaches to affinity chromatography.
A new armoury of protein purification tools is required to support rapid advances in high-throughput genomics and proteomics, which are predicted to lead to the discovery, isolation, characterisation and manufacture of a number of new biopharmaceutical proteins. Computer-aided molecular design, combinatorial (bio)chemistry and high-throughput screening techniques are now being exploited to identify highly selective ligands for use in the purification of these proteins by affinity chromatography.
Lowe
C R
CR
Institute of Biotechnology, University of Cambridge, Tennis Court Road, CB2 1QT, Cambridge, UK. crl1@biotech.cam.ac.uk
D002846:Chromatography, Affinity / Q000379:methods*; D020650:Combinatorial Chemistry Techniques; D008024:Ligands; D020033:Protein Isoforms / Q000737:chemistry / Q000302:isolation & purification
D008024:Ligands; D020033:Protein Isoforms