Rapid high-performance liquid chromatographic method for the simultaneous determination of retinol, alpha-tocopherol and beta-carotene in human plasma and low-density lipoproteins.
A reversed-phase HPLC method with diode-array detection was used to simultaneously determine retinol, alpha-tocopherol and beta-carotene in human plasma and low-density lipoproteins. An aliquot of sample was de-proteinized with ethanol containing beta-tocopherol acetate as internal standard, and the analytes were extracted twice with hexane. The solvent was evaporated to dryness under a stream of nitrogen and the residue was redissolved in methanol to be injected directly into the HPLC system. A multiple solvent system based on methanol, butanol and water at a flow-rate of 2 ml/min and held at 45 degrees C provided clear separation of these compounds in only 8 min. The method showed good linearity, precision and accuracy for all compounds. Owing to its simplicity, this method may be useful in routine clinical and epidemiological work.
Gimeno
E
E
Dpt. Nutricío i Bromatologia, Centre de Referència en Tecnologia dels Aliments, Facultat Fe Farmácia, Universitat de Barcelona, Spain.
Castellote
A I
AI
Lamuela-Raventós
R M
RM
de la Torre-Boronat
M C
MC
López-Sabater
M C
MC
D000328:Adult; D000368:Aged; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D008077:Lipoproteins, LDL / Q000737:chemistry*; D008297:Male; D008875:Middle Aged; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014801:Vitamin A / Q000032:analysis / Q000097:blood*; D024502:alpha-Tocopherol / Q000032:analysis / Q000097:blood*; D019207:beta Carotene / Q000032:analysis / Q000097:blood*
D008077:Lipoproteins, LDL; D019207:beta Carotene; D014801:Vitamin A; D024502:alpha-Tocopherol