Liposome mediated antigen delivery leads to induction of CD8+ T lymphocyte and antibody responses against the V3 loop region of HIV gp120.
There is general consensus that the use of whole viruses for the development of a vaccine against human immunodeficiency virus (HIV) would be unsafe. While currently available nonreplicating vaccines, composed of synthetic peptides or purified subunit antigens, can help in tricking the humoral immune responses, they fail to incite the other major arm of the immune defense system, i.e., cell mediated immunity (CMI). To overcome the difficulty in generating CMI, we have entrapped an immunodominant HIV envelope glycoprotein peptide in liposomes made up of fusogenic lipids isolated from Escherichia coli. We have established the role of fusogenic liposomes in stimulation of HIV-specific CD8+ cytotoxic T lymphocytes. Interestingly, the same liposomes elicit strong HIV-specific antibody production as well. Moreover, untoward manifestations such as skin damage or antibody production against lipid components were also not observed. Thus, E. coli lipid liposomes (escheriosomes) could prove to be a potent candidate vaccine, capable of eliciting both humoral and cell mediated immune responses against HIV infection.
Ahmad
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N
Department of Pharmaceutics, Faculty of Pharmacy, Jamia Hamdard, New Delhi, India.
Khan
M A
MA
Owais
M
M
D016915:AIDS Vaccines; D000818:Animals; D002478:Cells, Cultured; D003601:Cytotoxicity Tests, Immunologic; D004926:Escherichia coli / Q000737:chemistry; D005260:Female; D015483:HIV Antibodies / Q000276:immunology*; D015699:HIV Envelope Protein gp120 / Q000737:chemistry / Q000276:immunology*; D016056:Immunodominant Epitopes / Q000008:administration & dosage; D008081:Liposomes / Q000032:analysis; D008213:Lymphocyte Activation; D051379:Mice; D009842:Oligopeptides / Q000276:immunology*; D010455:Peptides / Q000008:administration & dosage / Q000276:immunology; D017434:Protein Structure, Tertiary; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D016915:AIDS Vaccines; D015483:HIV Antibodies; D015699:HIV Envelope Protein gp120; C433866:HKR peptide; D016056:Immunodominant Epitopes; D008081:Liposomes; D009842:Oligopeptides; D010455:Peptides