title: Immunocytochemical localization of nucleophosmin and RH-II/Gu protein in nucleoli of HeLa cells after treatment with actinomycin D.
abstract: HeLa cells were studied with the use of light microscopical immunocytochemistry to obtain more information on the nucleolar localization of nucleophosmin (B23 protein) and RH-II/Gu protein. In control cells these proteins are colocalized. After structural rearrangement of nucleoli induced by actinomycin D, nucleophosmin was present in the nucleolar periphery and nucleolar peripheral caps. In contrast, RH-II/Gu protein was observed mainly in central regions of nucleoli as a compact body. However, in some nucleoli the proteins colocalized in a small portion of the peripheral nucleolar cap. The proteins were also colocalized in micronucleoli but occasionally RH-II/Gu protein was absent or both proteins were not present.
authors.affiliation: Department of Pharmacology, Baylor College of Medicine, Houston, USA. karel.smetana@uhkt.cz
authors.forename: K
authors.initials: K
authors.lastname: Smetana
authors.forename: R
authors.initials: R
authors.lastname: Busch
authors.forename: P K
authors.initials: PK
authors.lastname: Chan
authors.forename: K
authors.initials: K
authors.lastname: Smetana
authors.forename: H
authors.initials: H
authors.lastname: Busch
mesh_terms: D002466:Cell Nucleolus / Q000737:chemistry*; D003609:Dactinomycin / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D007150:Immunohistochemistry; D009687:Nuclear Proteins / Q000032:analysis* / Q000737:chemistry; D019384:Nucleic Acid Synthesis Inhibitors / Q000494:pharmacology; D000090243:Nucleophosmin; D020365:RNA Helicases / Q000032:analysis* / Q000737:chemistry
chemical_list: C000716967:NPM1 protein, human; D009687:Nuclear Proteins; D019384:Nucleic Acid Synthesis Inhibitors; D000090243:Nucleophosmin; D003609:Dactinomycin; D020365:RNA Helicases