Sodium current in NG108-15 cell inhibited by scorpion toxin BmKAS-1 and restored by its specific monoclonal antibodies.
Effect on Na+ current of BmKAS-1, a novel polypeptide purified from the venom of Chinese scorpion Buthus martensi Karsch (BmK), has been investigated in differentiated NG108-15 cells by using patch-clamp whole-cell recording. Neutralizing effect of four monoclonal antibodies of BmKAS-1 (mAb #2, #3, #4, and #5) has also been observed. The results showed that Na+ current was irreversibly inhibited by BmKAS-1 and the inhibitory effect was abolished by mAb #2 and #5, but not by mAb #3 and #4.
Wu
Y
Y
Institute of Physiology, Chinese Academy of Sciences, People's Republic of China.
Ji
Y H
YH
Shi
Y L
YL
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D018929:Cell Culture Techniques; D004594:Electrophysiology; D010455:Peptides; D012604:Scorpion Venoms / Q000276:immunology / Q000494:pharmacology*; D015222:Sodium Channels / Q000187:drug effects*
D000911:Antibodies, Monoclonal; C119408:KAS-1 toxin, Buthus martensi; D010455:Peptides; D012604:Scorpion Venoms; D015222:Sodium Channels