[Presence of genetic rearrangements in E1/E2 regions of episomal HPV 16 isolates from cervical carcinomas 5-8].
OBJECTIVE
Analysis of genetic situations of E1/E2 region of episomal HPV 16 isolates from cervical carcinomas.


METHODS
Physical statues of HPV 16 DNAs from cervical carcinomas were analyzed by Southern blot hybridization. E1/E2 regions of episomal RPV 16 isolates were amplified by PCR, and the PCR products were cloned and sequenced.


RESULTS
Eight isolates from totally 28 cases showed extrachromosomal HPV 16 DNA. PCR cloned sequence analysis revealed that all the eight isolates contained intact E1/E2 regions, whereas 4 isolates had different ratios of genetic rearrangements, including insertion of E1 repeat sequence and several deletions. Only one case showed the same G deletion at position 1138 as HPV 16 prototype.


CONCLUSIONS
DNA rearrangements were present in E1/E2 regions of episomal HPV 16 isolates from cervical cancers and might play a role in tumorigenesis. Different viral genotypes in an identical tumor tissue might indicate that the tumor occurring at the same position of a patient may derive from two or even more cell clones.
Dong
X
X
Institute of Virology, Chinese Academy of Preventive Medicine, Beijing 100052, China.
Zhou
W
W
Pfister
H
H
D004279:DNA, Viral / Q000235:genetics*; D004268:DNA-Binding Proteins; D005260:Female; D015321:Gene Rearrangement; D006801:Humans; D009856:Oncogene Proteins, Viral / Q000235:genetics* / Q000302:isolation & purification; D027383:Papillomaviridae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D017421:Sequence Analysis; D002583:Uterine Cervical Neoplasms / Q000821:virology*
D004279:DNA, Viral; D004268:DNA-Binding Proteins; C093478:E1 protein, Human papillomavirus 16; C054855:E2 protein, Human papillomavirus type 16; D009856:Oncogene Proteins, Viral