title: Evaluation of the dentogingival area during early plaque formation.
abstract: BACKGROUND
  The aims of this investigation were to evaluate the pattern of supragingival plaque formation in the dentogingival area within 96 hours after abstinence from mechanical plaque control and to clinically analyze the gingival inflammatory response observed.
  
  
  METHODS
  Six male volunteers, 20 to 23 years of age, had their upper incisors and cuspids polished. Four independent periods of no mechanical plaque control--24, 48, 72, and 96 hours--were instituted. In each period, plaque was disclosed and standardized individual photographs were taken. Impressions were taken and replicas were analyzed by scanning electron microscopy. Gingival crevicular fluid (GCF) was collected at baseline and 96 hours. The height of the gingival papilla was measured at baseline, 24, and 96 hours.
  
  
  RESULTS
  Results showed the presence of a plaque-free zone (PFZ) along the gingival margin up to 72 hours. After 96 hours, there was a significant reduction in the presence of PFZ in the proximal thirds of the buccal surface when compared to the other experimental periods. At the end of the study, there was a significant increase in the GCF flow as well as edema of the interdental papilla, when compared to baseline values.
  
  
  CONCLUSIONS
  The PFZ observed during the initial phase was less apparent at the 96-hour period. At this time, there was an increase in the gingival inflammatory response, represented clinically by increased GCF flow and edema.
authors.affiliation: Graduate Program in Periodontology, Dental School, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: P
authors.initials: P
authors.lastname: Weidlich
authors.forename: M A
authors.initials: MA
authors.lastname: Lopes de Souza
authors.forename: R V
authors.initials: RV
authors.lastname: Oppermann
mesh_terms: D000328:Adult; D003773:Dental Plaque / Q000209:etiology* / Q000473:pathology / Q000648:ultrastructure; D004487:Edema / Q000209:etiology / Q000473:pathology; D005500:Follow-Up Studies; D005881:Gingiva / Q000473:pathology*; D005883:Gingival Crevicular Fluid / Q000737:chemistry; D005891:Gingivitis / Q000209:etiology / Q000473:pathology; D006801:Humans; D008297:Male; D008855:Microscopy, Electron, Scanning; D010781:Photography; D016628:Replica Techniques; D012394:Rosaniline Dyes; D013223:Statistics as Topic; D014070:Tooth / Q000473:pathology*
chemical_list: D012394:Rosaniline Dyes