title: Purification and characterization of fibroin from the tropical Saturniid silkworm, Antheraea mylitta.
abstract: The fibroin protein isolated from the posterior silkgland of the tropical Saturniid silkworm Antheraea mylitta, was solubilized in lithium dodecyl sulfate and purified by gel filtration. The major fraction from gel filtration was analyzed by SDS-PAGE under non-reducing and reducing conditions. One major protein band of ca 395 kDa was obtained under non-reducing conditions and a doublet band of approximately 197 kDa under reducing conditions. The appearance of a single spot in two-dimensional electrophoresis confirmed the purity of the protein indicating that it may be a homodimeric protein of two similar sized polypeptides. Amino acid composition analysis showed that, like other Saturniid fibroins, it is rich in glycine, alanine and serine amino acids. N-terminal amino acid sequence shows significant homology with other Antheraea species. The enzymatic deglycosylation analysis indicates that the fibroin protein is glycosylated and the oligosaccharides are O-linked to the protein backbone by N-acetylgalactoseamine moiety which conforms to a Core 1 mucin-type glycosylation pattern.
authors.affiliation: Department of Biotechnology, Indian Institute of Technology, Kharagpur 721302, India.
authors.forename: A
authors.initials: A
authors.lastname: Datta
authors.forename: A K
authors.initials: AK
authors.lastname: Ghosh
authors.forename: S C
authors.initials: SC
authors.lastname: Kundu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D012831:Bombyx / Q000737:chemistry*; D005349:Fibroins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D006031:Glycosylation; D008969:Molecular Sequence Data; D008970:Molecular Weight
chemical_list: D005349:Fibroins