Modification of apoptosis-inducing activity of gallic acid by saliva.
Gallic acid, a structural unit of tannin, induced apoptotic cell death, characterized by nuclear condensation and caspase activation in human oral tumor cell lines (HSC-2, HSG). Agarose gel electrophoresis demonstrated that gallic acid produced large DNA fragments in these cells, as well as in T-cell leukemia (MOLT-4) and erythroleukemia (K-562) cells, whereas it induced internucleosomal DNA fragmentation in human myelogenous leukemic cell lines (HL-60, ML-1, U-937, THP-1). This indicates that induction of internucleosomal DNA fragmentation or production of large DNA fragments depends on both target cells and inducers. Addition of total saliva dose-dependently reduced the cytotoxicity induction by gallic acid. These data suggested that the biological action of gallic acid might be modified by physiological fluids.
Aoki
K
K
Department of Operative Dentistry, Meikai University School of Dentistry, Sakado, Saitama, Japan.
Ishiwata
S
S
Sakagami
H
H
Kusama
K
K
Katayama
T
T
D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis; D020169:Caspases / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D053938:DNA Fragmentation; D004305:Dose-Response Relationship, Drug; D004587:Electrophoresis, Agar Gel; D004789:Enzyme Activation / Q000187:drug effects; D005707:Gallic Acid / Q000494:pharmacology*; D006801:Humans; D008956:Models, Chemical; D012463:Saliva / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D000975:Antioxidants; D005707:Gallic Acid; D020169:Caspases