title: Segregation of human neural stem cells in the developing primate forebrain.
abstract: Many central nervous system regions at all stages of life contain neural stem cells (NSCs). We explored how these disparate NSC pools might emerge. A traceable clone of human NSCs was implanted intraventricularly to allow its integration into cerebral germinal zones of Old World monkey fetuses. The NSCs distributed into two subpopulations: One contributed to corticogenesis by migrating along radial glia to temporally appropriate layers of the cortical plate and differentiating into lamina-appropriate neurons or glia; the other remained undifferentiated and contributed to a secondary germinal zone (the subventricular zone) with occasional members interspersed throughout brain parenchyma. An early neurogenetic program allocates the progeny of NSCs either immediately for organogenesis or to undifferentiated pools for later use in the "postdevelopmental" brain.
authors.affiliation: Department of Pediatrics, Children's Hospital, Harvard Medical School, 248 Enders Building, 300 Longwood Avenue, Boston, MA 02115, USA.
authors.forename: V
authors.initials: V
authors.lastname: Ourednik
authors.forename: J
authors.initials: J
authors.lastname: Ourednik
authors.forename: J D
authors.initials: JD
authors.lastname: Flax
authors.forename: W M
authors.initials: WM
authors.lastname: Zawada
authors.forename: C
authors.initials: C
authors.lastname: Hutt
authors.forename: C
authors.initials: C
authors.lastname: Yang
authors.forename: K I
authors.initials: KI
authors.lastname: Park
authors.forename: S U
authors.initials: SU
authors.lastname: Kim
authors.forename: R L
authors.initials: RL
authors.lastname: Sidman
authors.forename: C R
authors.initials: CR
authors.lastname: Freed
authors.forename: E Y
authors.initials: EY
authors.lastname: Snyder
mesh_terms: D000818:Animals; D016380:Brain Tissue Transplantation; D002454:Cell Differentiation; D019070:Cell Lineage; D002465:Cell Movement; D017690:Cell Transplantation; D002999:Clone Cells / Q000166:cytology / Q000637:transplantation; D006801:Humans; D008255:Macaca radiata / Q000196:embryology; D019579:Neocortex / Q000166:cytology* / Q000196:embryology*; D009474:Neurons / Q000166:cytology* / Q000637:transplantation; D016548:Prosencephalon / Q000166:cytology* / Q000196:embryology*; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology*; D014183:Transplantation, Heterologous