title: M1 receptor activation is a requirement for arecoline analgesia.
abstract: Arecoline. a drug obtained from the Areca Catechu L., induced a dose-dependent antinociception (0.3-1 mg kg(-1) i.p.) which was prevented by the muscarinic antagonists pirenzepine (0.1 microg per mouse i.c.v.) and S-(-)-ET-126 (0.01 microg per mouse i.c.v.). A dose-dependent inhibition of the antinociception induced by arecoline was observed after inactivation of the M1 gene by an antisense oligodeoxyribonucleotide (aODN). This effect was detected 24 h after the last i.c.v. injection of aODN. These results indicate that arecoline antinociception is mediated by the activation of central M1 muscarinic receptors.
authors.affiliation: Department of Pharmacology, University of Florence, Italy. ghelard@server1.pharm.unifi.it
authors.forename: C
authors.initials: C
authors.lastname: Ghelardini
authors.forename: N
authors.initials: N
authors.lastname: Galeotti
authors.forename: C
authors.initials: C
authors.lastname: Lelli
authors.forename: A
authors.initials: A
authors.lastname: Bartolini
mesh_terms: D018712:Analgesics, Non-Narcotic / Q000494:pharmacology*; D000818:Animals; D001115:Arecoline / Q000494:pharmacology*; D007274:Injections, Intraperitoneal; D007276:Injections, Intraventricular; D008297:Male; D051379:Mice; D018721:Muscarinic Agonists / Q000494:pharmacology*; D018727:Muscarinic Antagonists / Q000494:pharmacology; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D010648:Phenylacetates / Q000494:pharmacology; D010880:Piperidines / Q000494:pharmacology; D010890:Pirenzepine / Q000494:pharmacology; D051381:Rats; D011930:Reaction Time / Q000187:drug effects; D043643:Receptor, Muscarinic M1; D011976:Receptors, Muscarinic / Q000187:drug effects*
chemical_list: D018712:Analgesics, Non-Narcotic; C098725:ET 126; D018721:Muscarinic Agonists; D018727:Muscarinic Antagonists; D016376:Oligonucleotides, Antisense; D010648:Phenylacetates; D010880:Piperidines; D043643:Receptor, Muscarinic M1; D011976:Receptors, Muscarinic; D010890:Pirenzepine; D001115:Arecoline