Pluronic F-68 enhanced shoot regeneration in a potentially novel citrus rootstock.
The effects have been studied in vitro of the non-ionic, co-polymer surfactant, Pluronic F-68, on shoot regeneration and bud induction in epicotyl and cotyledon explants of Citrus depressa, a potential alternative rootstock to C. jambhiri for commercial Citrus. Supplementation of Murashige and Skoog (1962)-based, agar-solidified shoot regeneration/bud induction (SRBI) medium with 1.0 mg l(-1) 6-benzylaminopurine and 0.5% (w/v) Pluronic F-68 significantly (P < 0.05) increased mean fresh weight by a maximum of 60%, the proportion of explants exhibiting shoot/bud regeneration by 25% and the mean number of shoots per epicotyl explant by 184%, compared to untreated controls. Similarly, 0.5% (w/v) Pluronic F-68 significantly (P < 0.05) enhanced the mean percentage bud induction (91%) and the number of buds regenerated (>4-fold) per cotyledon explant. Interestingly, the mean fresh weight gain for both explants was unaffected across the range of concentrations (0.001-0.1% w/v) of Pluronic F-68 evaluated. Regenerated plants from epicotyl explants were transferred and acclimatized to glasshouse conditions.
Cancino
G O
GO
School of Life & Environmental Sciences, University of Nottingham, UK.
Gill
M I
MI
Anthony
P
P
Davey
M R
MR
Power
J B
JB
Lowe
K C
KC
D018533:Biomass; D002957:Citrus / Q000166:cytology; D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology; D018520:Plant Shoots / Q000166:cytology / Q000502:physiology*; D020442:Poloxamer / Q000494:pharmacology*; D012038:Regeneration / Q000187:drug effects*; D013501:Surface-Active Agents / Q000494:pharmacology*
D003470:Culture Media; D013501:Surface-Active Agents; D020442:Poloxamer