Cloning and sequencing of a novel human gene that encodes a putative target protein of Nesh-SH3.
By using a conventional two-hybrid technique with an Src homology 3 (SH3) domain of Nesh as the bait protein, a novel full-length cDNA was isolated and sequenced from a human placenta cDNA library. This cDNA consists of 3023 bp and has a predicted open reading frame that encodes 486 amino acids. It possesses an SH3 binding motif, a nuclear targeting sequence, and no catalytic domain. Overall, it has no similarity to known molecules involved in a signaling cascade. Polymerase Chair reaction-based mapping with both a monochromosomal hybrid panel and radiation hybrid cell panels localized the gene on human chromosome 3q12 near the marker D3S1271.
Matsuda
S
S
Department of Molecular Pathogenesis, Nagoya University School of Medicine, Japan. smatsuda@med.nagoya-u.ac.jp
Iriyama
C
C
Yokozaki
S
S
Ichigotani
Y
Y
Shirafuji
N
N
Yamaki
K
K
Hayakawa
T
T
Hamaguchi
M
M
D000595:Amino Acid Sequence; D020029:Base Pairing; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D002893:Chromosomes, Human, Pair 3; D003001:Cloning, Molecular; D005091:Exons; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020798:Two-Hybrid System Techniques; D018909:src Homology Domains / Q000235:genetics
C439569:ABI3BP protein, human; D002352:Carrier Proteins