title: The kamikaze approach to membrane transport.
abstract: Membrane transport proteins catalyse the movement of molecules into and out of cells and organelles, but their hydrophobic and metastable nature often makes them difficult to study by traditional means. Novel approaches that have been developed and applied to one membrane transport protein, the lactose permease from Escherichia coli, are now being used to study various other membrane proteins.
authors.affiliation: Howard Hughes Medical Institute, Department of Physiology, University of California, Los Angeles, California 90095-1662, USA. ronaldk@hhmi.ucla.edu
authors.forename: H R
authors.initials: HR
authors.lastname: Kaback
authors.forename: M
authors.initials: M
authors.lastname: Sahin-Tóth
authors.forename: A B
authors.initials: AB
authors.lastname: Weinglass
mesh_terms: D000478:Alkylation; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D001665:Binding Sites; D001692:Biological Transport / Q000502:physiology*; D002352:Carrier Proteins / Q000502:physiology*; D003545:Cysteine / Q000737:chemistry; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D029968:Escherichia coli Proteins; D005033:Ethylmaleimide / Q000494:pharmacology; D006801:Humans; D007785:Lactose / Q000378:metabolism; D008133:Long QT Syndrome / Q000378:metabolism; D008565:Membrane Proteins / Q000502:physiology*; D026901:Membrane Transport Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D008661:Metabolism, Inborn Errors / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009004:Monosaccharide Transport Proteins; D016297:Mutagenesis, Site-Directed; D011487:Protein Conformation; D011522:Protons; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D013329:Structure-Activity Relationship; D013439:Sulfhydryl Reagents / Q000494:pharmacology; D027981:Symporters
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D029968:Escherichia coli Proteins; C050221:LacY protein, E coli; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D009004:Monosaccharide Transport Proteins; D011522:Protons; D011993:Recombinant Fusion Proteins; D013439:Sulfhydryl Reagents; D027981:Symporters; C021499:lactose permease; D007785:Lactose; D003545:Cysteine; D005033:Ethylmaleimide