[Endothelial cell damage induced in vitro by subeschar tissue fluid].
OBJECTIVE
To assess the effect of subeschar tissue fluid (STF) on the function and structure of endothelial cell in vitro.


METHODS
Human umbilical vein endothelial cells (HUVEC) were incubated with STF obtained from patients in the early postburn stage. Then the morphological changes in EC were observed, and activity of EC and contents of LDH, 6-keto-PGF1 alpha in media were determined.


RESULTS
The activity of LDH and the content of 6-keto-PGF1 alpha were increased gradually after HUVEC incubated with STF, and were higher than that measured after HUVEC incubated with healthy volunteer's sera. Furthermore, activity of HUVEC decreased and they became deformed, showing shrinkage of the cell body with enlargement of intercellular space. In addition, small vacuoles appeared in cytoplasm with karyopyoknosis after 24 h.


CONCLUSIONS
STF can damage endothelial cell and might play an important role in the pathophysiological process of burn injury.
Li
Z
Z
Burn Unit, Nanfang Hospital, First Military Medical University, Guanzhou 510515.
Zhou
Y
Y
Rong
X
X
D015121:6-Ketoprostaglandin F1 alpha / Q000378:metabolism; D000293:Adolescent; D000328:Adult; D001826:Body Fluids / Q000502:physiology*; D002056:Burns / Q000150:complications*; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D004487:Edema / Q000209:etiology; D004730:Endothelium, Vascular / Q000166:cytology*; D005260:Female; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D014471:Umbilical Veins / Q000166:cytology* / Q000378:metabolism
D015121:6-Ketoprostaglandin F1 alpha; D007770:L-Lactate Dehydrogenase