title: Seroprevalence of Toxoplasma gondii infection in goats by the indirect haemagglutination, immunofluorescence and immunoenzymatic tests in the region of Uberlândia, Brazil.
abstract: A comparative study of the indirect haemagglutination (IHA), immunofluorescence (IFAT) and immunoenzymatic (ELISA) tests was carried out to determine the prevalence of Toxoplasma gondii antibodies in goats. One hundred seventy-four serum samples were obtained from four goat herds from the region of Uberlândia, State of Minas Gerais. The distribution of the animals, according to their origin, was as follow: 71 from herd I; 39 from herd II; 37 from herd III; and 27 from herd IV. Serum samples were analyzed by IHA, IFAT and ELISA, considering the reactivity of the serum samples at dilution > or = 1:64 as cut off titer for the three tests. A global seroprevalence of 18.4% was observed, with significantly higher positivity rate in the herd II (66.7%) and older animals (> 36 months). A high and significant positive correlation was found between the titers obtained by the IHA versus IFAT, IHA versus ELISA, and ELISA versus IFAT. Therefore, it can be concluded that the three analyzed tests have shown to be highly concordant and appropriate for epidemiological surveys of Toxoplasma infection in goats. Although the seroprevalence of T. gondii infection in goats is relatively low in this region as compared to other regions of the country, adequate management might be useful and essential to control the infection in the goat herds.
authors.affiliation: Departamento de Imunologia, Microbiologia e Parasitologia, Universidade Federal de Uberlândia, 38400-902 Uberlândia, MG, Brasil.
authors.forename: J F
authors.initials: JF
authors.lastname: Figueiredo
authors.forename: D A
authors.initials: DA
authors.lastname: Silva
authors.forename: D D
authors.initials: DD
authors.lastname: Cabral
authors.forename: J R
authors.initials: JR
authors.lastname: Mineo
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood* / Q000302:isolation & purification; D001938:Brazil / Q000453:epidemiology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary; D019084:Fluorescent Antibody Technique, Indirect / Q000379:methods / Q000662:veterinary; D006041:Goats; D006386:Hemagglutination Tests / Q000379:methods / Q000662:veterinary; D015995:Prevalence; D016036:Seroepidemiologic Studies; D014122:Toxoplasma / Q000276:immunology* / Q000302:isolation & purification; D014123:Toxoplasmosis / Q000175:diagnosis / Q000453:epidemiology / Q000276:immunology*
chemical_list: D000913:Antibodies, Protozoan