A screen of mammalian antibodies on snapper (Pagrus auratus, Sparidae) peripheral blood leukocytes reveals cross reactivity of an anti-human CD3 antibody with a population of mIg(-) cells.
Detailed immunological studies of the teleosts have been hampered by a lack of antibodies against cell-specific markers. Furthermore, where antibodies have been raised, in many instances they have been found to be species-specific. In comparison, many monoclonal and polyclonal antibodies exist with specificities for mammalian proteins and glycoproteins that effectively differentiate leukocyte sub-populations. In this study, we have tested a panel of 54 commercial antibodies against human and murine cell surface receptors for their ability to bind leukocytes isolated from the peripheral blood of snapper (Pagrus auratus). From this panel, one antibody, A452, which is specific for the intracytoplasmic tail of the epsilon (epsilon) chain of the T cell receptor-associated CD3 complex (CD3epsilon) bound to a subpopulation of peripheral blood leukocytes. Mutually exclusive counterstaining was observed when this antibody was used in conjunction with a monoclonal anti-snapper immunoglobulin antibody. This suggests that A452 may be binding to putative snapper T cells.
Cook
M T
MT
Department of Haematology, Hanson Centre for Cancer Research, Institute of Medical and Veterinary Science, Frome Road, Adelaide 5000, Australia.
Morrison
R N
RN
Wilkinson
R
R
Nowak
B F
BF
Hayball
P J
PJ
Hayball
J D
JD
D000818:Animals; D015703:Antigens, CD / Q000276:immunology; D017252:CD3 Complex / Q000276:immunology*; D003429:Cross Reactions; D000939:Epitopes; D007202:Indicators and Reagents; D007962:Leukocytes / Q000276:immunology*; D010473:Perciformes / Q000276:immunology*; D011948:Receptors, Antigen, T-Cell / Q000276:immunology*; D013045:Species Specificity
D015703:Antigens, CD; D017252:CD3 Complex; D000939:Epitopes; D007202:Indicators and Reagents; D011948:Receptors, Antigen, T-Cell