title: Effective bactericidal activity of tobramycin and vancomycin eluted from acrylic bone cement.
abstract: We studied the bioactivity of vancomycin and tobramycin eluted from methylmethacrylate bone cement. Aliquots of the drainage were obtained at 1, 6, 12 and 24 hours following total hip prosthetic implantation with vancomycin-tobramycin-loaded cement in 3 patients. The samples were analyzed with fluorescence polarization immunoassay and bioassay, using group B streptococcus for vancomycin and Escherichia coli for tobramycin. These bacteria were selected due to the effectiveness of vancomycin and poor effectiveness of tobramycin against group B streptococcus and conversely with E. coli. The immunodetection of vancomycin averaged 14 (1 hour), 9 (6 hours), 10 (12 hours) and 11 microg/mL (24 hours). The bioassay averaged 47, 36, 79 and 41 microg/mL (p = 0.03). The immunodetection of tobramycin averaged 43, 21, 18 and 14 microg/mL; and bioassay 30, 15, 15 and 12 microg/mL (p = 0.1). Both antibiotics eluted with a highly effective bactericidal activity. Our findings indicate that the presence of tobramycin has a synergistic-like effect on the bactericidal activity of vancomycin, which has not been previously reported. We recommend a combination of vancomycin and tobramycin with cement for the treatment of orthopedic infections caused by gram-positive organisms.
authors.affiliation: Department of Orthopedics, Hospital Italiano de Buenos Aires, Argentina. agdv@bifgoot.com
authors.forename: A
authors.initials: A
authors.lastname: González Della Valle
authors.forename: M
authors.initials: M
authors.lastname: Bostrom
authors.forename: B
authors.initials: B
authors.lastname: Brause
authors.forename: C
authors.initials: C
authors.lastname: Harney
authors.forename: E A
authors.initials: EA
authors.lastname: Salvati
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000494:pharmacology*; D019644:Arthroplasty, Replacement, Hip; D001681:Biological Assay; D001843:Bone Cements; D004926:Escherichia coli / Q000187:drug effects; D005260:Female; D005839:Gentamicins / Q000494:pharmacology; D006801:Humans; D008297:Male; D020366:Methylmethacrylate; D008875:Middle Aged; D011863:Radioimmunoassay; D013292:Streptococcus agalactiae / Q000187:drug effects; D014031:Tobramycin / Q000008:administration & dosage* / Q000494:pharmacology*; D014640:Vancomycin / Q000008:administration & dosage* / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D001843:Bone Cements; D005839:Gentamicins; D020366:Methylmethacrylate; D014640:Vancomycin; D014031:Tobramycin