Effects of lipofectin-antigen complexes on major histocompatibility complex class I-restricted antigen presentation pathway in murine dendritic cells and on dendritic cell maturation.
We previously reported that exogenous antigens complexed with the cationic liposome lipofectin (LF) were efficiently presented via major histocompatibility complex (MHC) class I molecules on pulsed dendritic cells (DCs) in vitro. In the present study, we demonstrated that MHC class I-restricted antigen presentation on DC2.4 cells, a murine immature DC line, treated with LF-antigen complexes was remarkably suppressed through the inhibition of endocytosis, proteasome catalysis, and Golgi transport. We also found that LF did not influence expression of interleukin-12 p40 mRNA, MHC molecules, or co-stimulatory molecules in DC2.4 cells. These findings suggest that an antigen-loading procedure using LF could enhance delivery of exogenous antigens to the classical MHC class I pathway in DCs, but it does not initiate DC maturation.
Okada
N
N
Department of Biopharmaceutics, Kyoto Pharmaceutical University, Japan. okada@mb.kyoto-phu.ac.jp
Saito
T
T
Mori
K
K
Masunaga
Y
Y
Fujii
Y
Y
Fujita
J
J
Fujimoto
K
K
Nakanishi
T
T
Tanaka
K
K
Nakagawa
S
S
Mayumi
T
T
Fujita
T
T
Yamamoto
A
A
D000111:Acetylcysteine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D017951:Antigen Presentation / Q000187:drug effects*; D000941:Antigens / Q000737:chemistry / Q000494:pharmacology*; D020126:Brefeldin A / Q000494:pharmacology; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002738:Chloroquine / Q000494:pharmacology; D003571:Cytochalasin B / Q000494:pharmacology; D003713:Dendritic Cells / Q000187:drug effects* / Q000276:immunology; D015395:Histocompatibility Antigens Class I / Q000276:immunology*; D008285:Major Histocompatibility Complex; D051379:Mice; D010047:Ovalbumin / Q000737:chemistry / Q000494:pharmacology; D010714:Phosphatidylethanolamines / Q000737:chemistry / Q000494:pharmacology*; D013997:Time Factors
D000941:Antigens; D015395:Histocompatibility Antigens Class I; D010714:Phosphatidylethanolamines; C067713:lactacystin; D020126:Brefeldin A; D003571:Cytochalasin B; C020888:1,2-dielaidoylphosphatidylethanolamine; D002738:Chloroquine; D010047:Ovalbumin; D000111:Acetylcysteine