Respiratory burst response of peritoneal leukocytes adhering to titanium and stainless steel.
Titanium sheets, made hydrophilic by oxidative cleaning or hydrophobic by treatment with butanol, and stainless steel sheets with different patterns of pores (straight phi = 0.8 mm) were implanted into the peritoneal cavity of mice. The implants were removed after 2 h, and the surface-adhering leukocytes were stained with propidium iodide and fluorescein diacetate to quantitate cell adhesion and to indicate the presence of leaks in the cell membrane. The ability of the surface-adhering leukocytes to mount a respiratory burst response after stimulation with PMA or zymosan was measured by chemiluminiscence. The results show that stainless steel without pores induces membrane leakage in 80% of the surface-adhering leukocytes compared with 65% of cells adhering to porous steel. Hydrophilic titanium induces membrane leakage in 48% of the surface-adhering leukocytes compared with 19% of cells adhering to hydrophobic titanium. The respiratory burst response of the surface-adhering leukocytes stimulated with PMA was attenuated on stainless steel and hydrophilic titanium compared with hydrophobic titanium. Thus, butanol treatment of titanium and pores in stainless steel increase the biocompatibility of the materials.
Nygren
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H
Department of Applied Cell Biology, Institute of Anatomy and Cell Biology, University of Göteborg, Sweden. Hakan.Nygren@anatcell.gu.se
Hrustic
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E
Karlsson
C
C
Oster
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L
D000818:Animals; D001672:Biocompatible Materials; D000440:Butanols / Q000737:chemistry; D002448:Cell Adhesion / Q000502:physiology*; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D007962:Leukocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D008163:Luminescent Measurements; D051379:Mice; D010529:Peritoneal Cavity / Q000166:cytology; D019736:Prostheses and Implants; D016897:Respiratory Burst / Q000502:physiology*; D013193:Stainless Steel; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014025:Titanium; D015054:Zymosan / Q000494:pharmacology
D001672:Biocompatible Materials; D000440:Butanols; D013193:Stainless Steel; D015054:Zymosan; D014025:Titanium; D013755:Tetradecanoylphorbol Acetate