Partial characterization of a bacteriocin produced by Lactobacillus delbrueckii subsp. lactis UO004, an intestinal isolate with probiotic potential.
OBJECTIVE
The partial characterization of a bacteriocin produced by a human Lactobacillus delbrueckii isolate with probiotic potential.


RESULTS
A bacterocin, UO004, was partially purified by cation exchange followed by a hydrophobic interaction column, biochemically characterized and the N-terminal region sequenced. Bacteriocin UO004 was found to be a hydrophobic, heat-stable polypeptide with an apparent molecular mass of 6 kDa. It was also stable and active over a wide pH range.


CONCLUSIONS
The active compound was proteinaceous, heat-stable, and had a bactericidal (and bacteriolytic) mode of action on a limited number of micro-organisms. Such a narrow spectrum of activity is typical for bacteriocins produced by intestinal Lactobacillus.


CONCLUSIONS
Bacteriocin UO004 from a probiotic strain is a new compound that does not share any homology with any other known lactic acid bacteria bacteriocin. Furthermore, Lact. delbrueckii is regarded as a suitable starter for the production of fermented milks.
Boris
S
S
Area de Microbiología, Departamento de Biología Funcional, Universidad de Oviedo, Spain.
Jiménez-Díaz
R
R
Caso
J L
JL
Barbés
C
C
D000818:Animals; D001430:Bacteriocins / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D005285:Fermentation; D006358:Hot Temperature; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007223:Infant; D007422:Intestines / Q000382:microbiology*; D007778:Lactobacillus / Q000145:classification / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism*; D008826:Microbial Sensitivity Tests; D008892:Milk / Q000382:microbiology; D008970:Molecular Weight; D019936:Probiotics / Q000302:isolation & purification / Q000378:metabolism*; D020539:Sequence Analysis, Protein
D001430:Bacteriocins