Glycol methacrylate: an alternative method for embedding subcutaneous implants.
The aim of this investigation was to study the use of glycol methacrylate (GMA) as an embedding material for rat subcutaneous tissue, which received implants of tubes with endodontic sealer. After fixation, the specimens were dehydrated in a growing alcohol series up to 95%, immersed in infiltration GMA solution and then in embedding solution. The blocks were cut into 3.0 microm sections and stained with hematoxylin-eosin. The quality of cell definition and staining allowed a quantitative analysis of the cells infiltrated in the end of the tubes. It was also possible to identify each type of inflammatory cell. Moreover, it was possible to distinguish clearly chronic from acute inflammatory cells. The GMA technique is easy to execute and reproducible, and provides a better definition of tissue cells, thus permitting definition of the degree of the inflammatory process. Therefore, it is an excellent alternative for the evaluation of the biocompatibility of endodontic sealers.
Gomes-Filho
J E
JE
Dental Faculty of Piracicaba, Brazil.
Gomes
B P
BP
Zaia
A A
AA
Novaes
P D
PD
Souza-Filho
F J
FJ
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D004396:Coloring Agents; D003890:Desiccation; D004801:Eosine Yellowish-(YS); D000431:Ethanol; D005456:Fluorescent Dyes; D015726:Giant Cells / Q000473:pathology; D006416:Hematoxylin; D007101:Immersion; D007249:Inflammation; D007962:Leukocytes / Q000473:pathology; D008214:Lymphocytes / Q000473:pathology; D008264:Macrophages / Q000473:pathology; D008689:Methacrylates; D008867:Microtomy; D010950:Plasma Cells / Q000473:pathology; D051381:Rats; D012387:Root Canal Filling Materials / Q000737:chemistry*; D012867:Skin / Q000473:pathology*; D012997:Solvents; D016610:Tissue Embedding / Q000379:methods*; D016707:Tissue Fixation
D001672:Biocompatible Materials; D004396:Coloring Agents; D005456:Fluorescent Dyes; D008689:Methacrylates; D012387:Root Canal Filling Materials; D012997:Solvents; D000431:Ethanol; C005044:hydroxyethyl methacrylate; D004801:Eosine Yellowish-(YS); D006416:Hematoxylin