Three new MHC haplotypes in broiler breeder chickens.
Six distinct serotypes of the chicken B blood group system (which encodes the major histocompatibility complex) were identified in a commercial broiler breeder line (Line C). The B serotypes were compared by B-G restriction fragment length polymorphism (RFLP) analysis, allele-specific PCR typing test for B-LBII family genes and nucleotide sequence analysis of expressed B-F and B-LBII family genes. The results indicated the existence of seven distinct B haplotypes. Nucleotide sequence analysis demonstrated that three of the Line C haplotypes encode new B-F and B-LB alleles.
Livant
E J
EJ
Department of Poultry Science, Auburn University Auburn, AL 36849, USA.
Zheng
D
D
Johnson
L W
LW
Shi
W
W
Ewald
S J
SJ
D000483:Alleles; D000818:Animals; D001483:Base Sequence; D002645:Chickens / Q000235:genetics* / Q000276:immunology*; D006239:Haplotypes / Q000235:genetics; D008285:Major Histocompatibility Complex; D008969:Molecular Sequence Data; D005810:Multigene Family; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D012703:Serotyping