title: [Changes in the content and activity of HSC70/HSP68, proteinases and phosphatases during liver regeneration].
abstract: This article reports changes in acid and alkaline phosphatases (ACP and AKP), constitutive heat shock protein 70/induced heat shock protein 68 (HSC70/HSP68) and acid and neutral proteinases during liver regeneration (0-144 h) after 2/3 hepatectomy (partial hepatectomy, PH). Both ACP and AKP had two active peaks at 4 and 48 h, 16 and 96 h, respectively, which were followed by significant decrease. The content of HSC70/HSP68 also showed two peaks (16 and 96 h), of which the content after the second peak decreased more obviously than after the first peak. Moreover a 90 kD neutral proteinase was induced at the time from 2 to 6 h and a 27 kD acid proteinase was induced at 36 h. The results suggest that the ACP and the 90 kD neutral proteinase may participate in activating hepatocytes from G0-phase into G1-phase, and AKP and HSC70/HSP68 may play a role mainly in DNA synthesis, cellular metabolism and proliferation. Furthermore 27 kD acid proteinase may be involved in re-differentiation of hepatocytes and reconstruction of liver tissue.
authors.affiliation: College of Life Science, Henan Normal University, Xinxiang 453002.
authors.forename: C S
authors.initials: CS
authors.lastname: Xu
authors.forename: M
authors.initials: M
authors.lastname: Xia
authors.forename: A L
authors.initials: AL
authors.lastname: Lu
authors.forename: X Y
authors.initials: XY
authors.lastname: Li
authors.forename: Y H
authors.initials: YH
authors.lastname: Li
authors.forename: X Y
authors.initials: XY
authors.lastname: Zhao
authors.forename: Y H
authors.initials: YH
authors.lastname: Hu
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D010450:Endopeptidases / Q000378:metabolism*; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D008115:Liver Regeneration / Q000502:physiology*; D008297:Male; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D018840:HSP70 Heat-Shock Proteins; C097210:Hspa1a protein, rat; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D010450:Endopeptidases