Phenotyping cytochromes P450 with monoclonal antibodies.
Monoclonal antibodies (MAbs) to cytochrome P-450 isozymes can be used to phenotype tissues for epitope-specific cytochrome P-450 content. MAbs that inhibit specific cytochrome P-450 dependent drug or carcinogen reactions are useful tools for quantitative measurement of the individual or classes of cytochromes P-450 that catalyze these reactions. This method has been applied successfully to animal as well as human tissues. Radioimmunoassays based on MAbs have been developed and provide a rapid and efficient means for detecting cytochromes P-450 independent of functional enzyme activity. In addition, MAbs coupled to a Sepharose support can be used to immunopurify cytochromes P-450 in a procedure that is more rapid and efficient than conventional purification schemes. MAbs add a new dimension to analyses of cytochrome P-450 multiplicity and will find numerous applications in elucidation of the relationship between cytochrome P-450 phenotype and carcinogen or drug metabolism.
Friedman
F K
FK
Laboratory of Molecular Carcinogenesis, National Cancer Institute, Bethesda, Maryland 20205, USA.
Park
S S
SS
Fujino
T
T
Song
B J
BJ
Robinson
R C
RC
West
D
D
Radkowsky
A K
AK
Miller
H
H
Gelboin
H V
HV
D000818:Animals; D000911:Antibodies, Monoclonal; D003577:Cytochrome P-450 Enzyme System / Q000276:immunology* / Q000302:isolation & purification; D018604:Epitope Mapping; D006801:Humans; D007527:Isoenzymes; D010641:Phenotype; D011863:Radioimmunoassay
D000911:Antibodies, Monoclonal; D007527:Isoenzymes; D003577:Cytochrome P-450 Enzyme System