Proteins from fish eggs that protect DNA from acid precipitation and inhibit DNA synthesis.
We partially characterized proteins that inhibit DNA acid precipitation from various fish eggs (Sparus aurata, Dicentrarchus labrax, Mugil cephalus and Zeus faber). The active proteins were purified by acetone fractionation. The activity was found to be heat resistant. Of bivalent cations tested only Co(2+) and Cu(2+) exerted a profound promoting effect in the activity from all fish. The protein fraction from Sparus aurata inhibited DNA synthesis in PCR performed by different DNA polymerases. The possible role of DNA protective proteins in fish egg physiology is discussed.
Tsamis
V
V
School of Agriculture, University of Thessaly, Fitoko Neas Ionias, 38446, Magnisia, Greece.
Mamuris
Z
Z
Panagiotaki
P
P
Kouretas
D
D
D000818:Animals; D004247:DNA / Q000096:biosynthesis*; D004249:DNA Damage; D004259:DNA-Directed DNA Polymerase / Q000302:isolation & purification* / Q000378:metabolism; D004531:Eggs; D005260:Female; D005399:Fishes / Q000502:physiology*; D005590:Fractional Precipitation; D006863:Hydrogen-Ion Concentration; D016133:Polymerase Chain Reaction; D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification; D012995:Solubility; D013696:Temperature; D000509:alpha-Fetoproteins
D011506:Proteins; D000509:alpha-Fetoproteins; D004247:DNA; D004259:DNA-Directed DNA Polymerase