[The hemoprotective effect of platelet factor 4 (PF4) and tetrapeptide AcSDKP].
OBJECTIVE
To study the effects of platelet factor 4(PF4) and tetrapeptide N-acetyl-Ser-Asp-Lys-Pro(AcSDKP) on hemopoietic progenitors in mice treated with 5-Fluorouracil (5-FU).


METHODS
Mice were injected with PF4 (40 micrograms/kg) or AcSDKP (4 micrograms/kg) twice at 6 h intervals, and 20 h after the second injection they were given one injection of 5-FU (150 mg/kg), and the high proliferative potential-colony forming cell (HPP-CFC), burst-forming unit erythroid (BFU-E), colony forming unit megakaryocyte (CFU-MK), and megakaryocyte (MK) were examined 6, 8 and 13 days later.


RESULTS
The administration of PF4 or AcSDKP resulted in a significant increase of the number of HPP-CFC on days 6-8 and BFU-E and CFU-GM on day 8 when compared to 5-FU alone. Furthermore, PF4 was found to increase significantly the number of CFU-MK and MK on day 8, which was not observed with AcSDKP.


CONCLUSIONS
PF4 or AcSDKP accelerate the recovery in vivo of HPP-CFC, CFU-GM and BFU-E after 5-FU treatment but their effect may be different on megakaryocytic progenitors. Both molecules may have a hemoprotective effect against chemotherapeutic agents.
Han
Z
Z
State Key Laboratory of Experimental Hematology, Institute of Hematology, CAMS and PUMC, Tianjin 300020.
Cai
Y
Y
Aidoudi
S
S
D000818:Animals; D000964:Antimetabolites, Antineoplastic / Q000494:pharmacology; D005472:Fluorouracil / Q000494:pharmacology; D006410:Hematopoiesis / Q000187:drug effects*; D006412:Hematopoietic Stem Cells / Q000187:drug effects*; D008297:Male; D051379:Mice; D009842:Oligopeptides / Q000494:pharmacology*; D010978:Platelet Factor 4 / Q000494:pharmacology*
D000964:Antimetabolites, Antineoplastic; D009842:Oligopeptides; D010978:Platelet Factor 4; C058504:goralatide; D005472:Fluorouracil