Cell-mediated immune response in megacolon from patients with chronic Chagas' disease.
OBJECTIVE
The mechanisms that control chronic infection in vivo and the immunologic mechanisms involved in the pathogenesis of chagasic megacolon are not completely characterized. Although autoimmunity may play a role in the pathogenesis of Chagas' disease, recent studies, both in mice and in humans, suggest a positive association of tissue parasitism, inflammation, and severity of lesions. The aim of this study was to evaluate the role of inflammatory cells and the subclasses of lymphocytes involved in neuropathic lesions in the colon of patients who underwent resection for advanced megacolon.


METHODS
Specimens from 23 patients were selected based on histopathologic analysis. Paraffin-embedded tissue blocks were sectioned and evaluated by immunohistochemistry for cluster of differentiation 3, cluster of differentiation 8, cluster of differentiation 20, and natural killer cell antibodies by an avidin-biotin peroxidase method.


RESULTS
Almost all myenteric plexuses were damaged, characterized by degenerative changes, necrosis of ganglion cells, and inflammatory response. Mild lymphocytic infiltration around degenerated and normal ganglion cells was observed in all cases. Collagen fibers and mononuclear cells surrounded some ganglion cells. Most of the inflammatory cells were lymphocytes, identified as cluster of differentiation 3-positive cells. Cluster of differentiation 8-positive lymphocytes were associated with degenerated ganglion cells. Natural killer cell antibodies were detected in a lower proportion of cells and were distributed between muscle layers or in proximity to the myenteric plexus. All these findings were also observed in the submucosal plexus. Cluster of differentiation 20-positive lymphocytes were not present in muscle layers or in the vicinity of either plexus.


CONCLUSIONS
Pathogenesis of the megacolon is based on a continuous process of ganglion cell damage with participation of T lymphocytes expressing cluster of differentiation 8 and natural killer cell membrane antigens. B lymphocytes do not take part in the chronic inflammatory reaction.
Corbett
C E
CE
Department of Pathology, School of Medicine, University of São Paulo, Av. Dr. Amaldo 455, 01246.903 São Paulo, Brazil.
Ribeiro
U
U
Prianti
M G
MG
Habr-Gama
A
A
Okumura
M
M
Gama-Rodrigues
J
J
D000917:Antibody Formation; D002454:Cell Differentiation; D014355:Chagas Disease / Q000150:complications* / Q000276:immunology; D002908:Chronic Disease; D006801:Humans; D007150:Immunohistochemistry; D007249:Inflammation; D007694:Killer Cells, Natural / Q000276:immunology; D008531:Megacolon / Q000209:etiology / Q000276:immunology* / Q000503:physiopathology; D013601:T-Lymphocytes / Q000276:immunology*