DNA cloning and sequence analysis of chicken AFLP.
Amplified fragment length polymorphisms (AFLP) have been shown to be useful for linkage mapping in chickens and other domestic animals. It is often desirable to convert AFLP bands to sequence-tagged site (STS) markers, in particular, so that AFLP-based linkage information can be integrated with recombinant DNA clone-based maps. Sixteen chicken AFLP bands were excised from gels, re-amplified, cloned and analysed. All inserts proved to be EcoRI-TaqI fragments, which suggests that unlabelled TaqI-TaqI AFLP fragments do not amplify well, and therefore do not significantly contaminate AFLP bands. For eight of the AFLP, the cloned fragment was used to probe blots of AFLP reaction fingerprints, confirming that the predominant DNA clone indeed contained the polymorphic fragment. Flanking regions of selected AFLP fragments were isolated using Vectorette cloning. The results obtained suggest that the these chicken AFLP most commonly arise from sequence polymorphism at or near the TaqI site.
Knorr
C
C
Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI 48824, USA.
Cheng
H H
HH
Dodgson
J B
JB
D000818:Animals; D001483:Base Sequence; D002645:Chickens / Q000235:genetics*; D002874:Chromosome Mapping; D003001:Cloning, Molecular / Q000379:methods; D015246:Deoxyribonuclease EcoRI; D005819:Genetic Markers; D011110:Polymorphism, Genetic; D015183:Restriction Mapping; D016324:Sequence Tagged Sites
D005819:Genetic Markers; D015246:Deoxyribonuclease EcoRI