[Inhibition of the expression of Epstein-Barr virus antigens in vitro by Chinese medicine compound "Dongxia wan"].
OBJECTIVE
To investigate the effect of Chinese medicine "Dongxia wan" on the expression of Epstein-Barr virus (EBV) antigens in vitro.


METHODS
Cell culture and indirect immunoenzyme methods were used.


RESULTS
When Raji and B95-8 cells were cultured with 20-80 microg/'ml of "Dongxia wan", the expression of EBV early antigen (EBV-EA) and EBV capsid antigen (EBV-VCA) induced by croton oil and n-butyric acid and the natural expression of EBV-VCA were all inhibited significantly. The inhibition rates were 19.3% - 49.84%, 34.63% - 45.61% and 21.67% - 47.78%, respectively. When Raji and B95-8 cells were pretreated with "Dongxia wan" the expression of EBV-EA and EBV-VCA were also inhibited obviously.


CONCLUSIONS
Chinese medicine "Dongxia wan" can inhibit the expression of EBV antigens in target cells, it may be used to the prevention and treatment of EBV related diseases.
Huang
P
P
Institute of Oncology, Guangdong Medical College, Zhanjiang 524023, China.
Deng
H
H
Zhao
M
M
D000956:Antigens, Viral / Q000096:biosynthesis*; D036022:Capsid Proteins / Q000096:biosynthesis*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004854:Herpesvirus 4, Human / Q000187:drug effects* / Q000276:immunology; D066298:In Vitro Techniques
D000956:Antigens, Viral; D036022:Capsid Proteins; D004365:Drugs, Chinese Herbal; C041788:Epstein-Barr viral capsid antigen; C033305:Epstein-Barr virus early antigen