Metabolic and hemodynamic response of adipose tissue to angiotensin II.
OBJECTIVE
Recent studies have revealed the presence of a local renin-angiotensin system in adipose tissue. To examine the possible role of this system in adipose tissue, we performed microdialysis studies on the effect of angiotensin II (Ang II) on blood flow and metabolism in abdominal subcutaneous adipose tissue (aSAT) and femoral subcutaneous adipose tissue (fSAT) in young healthy men.


METHODS
Using the microdialysis technique, two different protocols were run perfusion with Ringer's solution + 50 mM ethanol with the subsequent addition of 125, 250, and 500 microg/liter Ang II (n = 8) and Ringers's solution + 50 mM ethanol with the subsequent addition of isoproterenol (1 microM) alone and in combination with 500 microg/liter Ang II (n = 6). Dialysate concentrations of ethanol, glycerol, glucose, and lactate were measured for estimating blood flow (ethanol dilution technique), lipolysis, and glycolysis, respectively.


RESULTS
Perfusion with Ang II resulted in a dose-dependent decrease in blood flow (fSAT > aSAT), lipolysis (fSAT > aSAT), and glucose uptake (fSAT = aSAT). Isoproterenol increased blood flow and lipolysis at both sites and those effects could be returned to baseline values by the addition of Ang II in aSAT but not fSAT.


CONCLUSIONS
In conclusion, our data indicate that in addition to its well-known vasoconstricting effect, Ang II inhibits lipolysis in adipose tissue, whereby femoral fat depots seem to be more sensitive to this effect than abdominal depots.
Boschmann
M
M
Department of Biochemistry and Physiology of Nutrition, German Institute of Human Nutrition, Potsdam, Germany. boschman@www.dife.de
Ringel
J
J
Klaus
S
S
Sharma
A M
AM
D000273:Adipose Tissue / Q000033:anatomy & histology / Q000098:blood supply / Q000378:metabolism*; D000328:Adult; D000804:Angiotensin II / Q000008:administration & dosage*; D004305:Dose-Response Relationship, Drug; D004734:Energy Metabolism / Q000187:drug effects*; D006801:Humans; D007545:Isoproterenol / Q000494:pharmacology; D008066:Lipolysis / Q000187:drug effects*; D008297:Male; D017551:Microdialysis / Q000379:methods; D010477:Perfusion; D012039:Regional Blood Flow / Q000187:drug effects; D012084:Renin-Angiotensin System / Q000502:physiology*; D013566:Sympathomimetics / Q000494:pharmacology
D013566:Sympathomimetics; D000804:Angiotensin II; D007545:Isoproterenol