Investigation of the metabolism of substance P in rat striatum by microdialysis sampling and capillary electrophoresis with laser-induced fluorescence detection.
The metabolism of substance P (SP) was investigated in rat striatum using in vivo microdialysis. Substance P was perfused for 5 h at 0.2 microl/min, and its metabolism was followed for over 13 h. The resulting samples were derivatized precolumn with naphthalene-2,3-dicarboxaldehyde (NDA)/cyanide, separated and detected by cyclodextrin-modified electrokinetic chromatography with laser-induced fluorescence detection (CDMEKC-LIF). Substance P rapidly degraded to form the fragments (3-11), (1-9), (1-4) and, to a lesser extent, (1-7). The metabolites reached steady-state levels 2-3 h after addition of SP.
Freed
A L
AL
Department of Pharmaceutical Chemistry and the Center for Bioanalytical Research, University of Kansas, 2095 Constant Avenue, Lawrence, KS 66047, USA.
Cooper
J D
JD
Davies
M I
MI
Lunte
S M
SM
D000818:Animals; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000379:methods*; D005453:Fluorescence; D007202:Indicators and Reagents / Q000032:analysis; D007834:Lasers; D008297:Male; D017551:Microdialysis / Q000379:methods*; D009281:Naphthalenes / Q000032:analysis; D017072:Neostriatum / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013373:Substance P / Q000378:metabolism*
D007202:Indicators and Reagents; D009281:Naphthalenes; D013373:Substance P; C027717:2,3-naphthalenedicarboxaldehyde