Detection of tyrosine phosphorylated proteins in Trichinella spiralis L1 larvae.
Western-blotting analysis showed the presence of tyrosine phosphorylated proteins in crude extracts of T. spiralis larvae and these phosphorylated proteins were located by immunofluorescence on the striations of the larval cuticle. The patterns of phosphorylated proteins were modified when larvae were incubated with bile.
Allegretti
S
S
Signalisation cellulaire et Parasites, EA 2499/INSERM U529, Hôpital Cochin, Université R. Descartes, 27, rue du Faubourg St.-Jacques, 75014, Paris, France.
Beaumont
V
V
Robert-Gangneux
F
F
Creuzet
C
C
Roisin
M P
MP
Dupouy-Camet
J
J
D000818:Animals; D015153:Blotting, Western; D005455:Fluorescent Antibody Technique; D015801:Helminth Proteins / Q000032:analysis*; D007814:Larva; D051379:Mice; D018482:Muscle, Skeletal / Q000469:parasitology; D010750:Phosphoproteins / Q000032:analysis*; D019000:Phosphotyrosine / Q000032:analysis*; D017160:Trichinella spiralis / Q000737:chemistry / Q000166:cytology / Q000502:physiology*
D015801:Helminth Proteins; D010750:Phosphoproteins; D019000:Phosphotyrosine