Analysis of phenolic constituents of biological interest in red wines by high-performance liquid chromatography.
We describe a reversed-phase HPLC method that uses gradient elution and diode array detection to determine four biologically active phenolic constituents of red wines: gallic acid, trans-resveratrol, quercetin and rutin. The method permits direct injection without sample pre-treatment. ODS Hypersil served as the stationary phase; the gradient was formed by acetic acid, methanol, and water. Each analysis required an equilibration period of 10 min and a run time of 50 min for completion. Previously, total phenols were analysed according to the Folin-Ciocalteu method, using gallic acid as the standard, and the results are given as gallic acid equivalent.
López
M
M
Department of Physical Chemistry, Faculty of Pharmacy, University of Granada, Spain.
Martínez
F
F
Del Valle
C
C
Orte
C
C
Miró
M
M
D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D010636:Phenols / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet; D014920:Wine / Q000032:analysis*
D010636:Phenols