title: Differential expression levels of Par-4 in melanoma.
abstract: The pro-apoptotic prostate apoptosis response-4 gene product Par-4 sensitizes prostate cells to the induction of programmed cell death. In this study we examined Par-4 expression in human melanoma cell lines and melanoma metastases. The heterogeneous expression detected prompted us to investigate the biological relevance of Par-4 in a human melanoma xenotransplantation model. Overexpression of Par-4 by transfection decreased tumour development in xenotransplanted A375-C6 melanoma cells in SCID mice and correlated to an increase in tumour cell apoptosis. These data suggest that high expression of the pro-apoptotic protein Par-4 could qualify as a prognostic marker in human melanoma.
authors.affiliation: Department of Clinical Pharmacology, Section of Experimental Oncology/Molecular Pharmacology, University of Vienna, Währinger Gürtel 18-20, A-1090 Vienna, Austria.
authors.forename: T
authors.initials: T
authors.lastname: Lucas
authors.forename: B
authors.initials: B
authors.lastname: Pratscher
authors.forename: S
authors.initials: S
authors.lastname: Krishnan
authors.forename: D
authors.initials: D
authors.lastname: Fink
authors.forename: P
authors.initials: P
authors.lastname: Günsberg
authors.forename: M
authors.initials: M
authors.lastname: Wolschek
authors.forename: V
authors.initials: V
authors.lastname: Wacheck
authors.forename: T
authors.initials: T
authors.lastname: Muster
authors.forename: I
authors.initials: I
authors.lastname: Romirer
authors.forename: K
authors.initials: K
authors.lastname: Wolff
authors.forename: H
authors.initials: H
authors.lastname: Pehamberger
authors.forename: H G
authors.initials: HG
authors.lastname: Eichler
authors.forename: V M
authors.initials: VM
authors.lastname: Rangnekar
authors.forename: B
authors.initials: B
authors.lastname: Jansen
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D051017:Apoptosis Regulatory Proteins; D014408:Biomarkers, Tumor / Q000235:genetics; D015153:Blotting, Western; D002352:Carrier Proteins / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D047908:Intracellular Signaling Peptides and Proteins; D008545:Melanoma / Q000235:genetics* / Q000473:pathology*; D051379:Mice; D016513:Mice, SCID; D009368:Neoplasm Transplantation; D011379:Prognosis; D014162:Transfection; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured
chemical_list: D051017:Apoptosis Regulatory Proteins; D014408:Biomarkers, Tumor; D002352:Carrier Proteins; D047908:Intracellular Signaling Peptides and Proteins; C101296:prostate apoptosis response-4 protein