Rapid diagnosis of cytomegalovirus infection in congenital neonates.
Immunostaining was compared with PCR for diagnosis of congenital CMV infection. IgM and IgG antibody assays were also performed in parallel. Immunostaining gave sensitivity and specificity of 60% and 97% respectively. Correlations among immunostaining, PCR and the presence of IgM antibody was reported. Immunostaining can be used for early diagnosis of congenital CMV infection in parallel with detection of IgM antibody.
Amarapal
P
P
Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
Tantivanich
S
S
Pengsa
K
K
Chotekiate
U
U
Kaolueng
S
S
Balachandra
K
K
Janeprasert
J
J
Samkosade
R
R
D001483:Base Sequence; D003586:Cytomegalovirus Infections / Q000151:congenital / Q000175:diagnosis*; D017931:DNA Primers; D004797:Enzyme-Linked Immunosorbent Assay; D006367:HeLa Cells; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology; D007231:Infant, Newborn; D015997:Neonatal Screening; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D013785:Thailand
D017931:DNA Primers; D007074:Immunoglobulin G; D007075:Immunoglobulin M