title: Changes in peripheral blood leukocyte populations in pigs with natural postweaning multisystemic wasting syndrome (PMWS).
abstract: The objective of the present study was to analyze, by flow cytometry, changes in PBMC subsets in pigs having postweaning multisystemic wasting syndrome (PMWS), a new condition associated to porcine circovirus type 2 (PCV2) infection. Thirteen acutely PMWS affected pigs were selected from a farm seronegative to porcine reproductive and respiratory syndrome virus (PRRSV) and to Aujeszky's disease virus (ADV); 11 clinically healthy pigs were selected from a high health farm with no history of PMWS and free of the major swine pathogens, and used as a control group. All pigs were necropsied, and tissue samples were fixed in formalin; blood with EDTA anticoagulant was used to perform the flow cytometric analysis. PBMC were incubated with mAb against porcine CD3, CD4, CD8, CD25, CD45, IgM, SWC3, and SLA-Class II. Flow cytometric analysis showed substantial changes in leukocyte subsets in the peripheral blood of PMWS-affected pigs, which were characterized by an increase of monocytes, a reduction of T (mainly CD4(+)) and B-lymphocytes, and the presence of low-density immature granulocytes. Altogether, these changes would suggest an inability of acutely PMWS-affected pigs to mount an effective immune response.
authors.affiliation: Departament de Sanitat i Anatomia Animals, Centre de Recerca en Sanitat Animal, Universitat Autònoma de Barcelona, Bellaterra, 08193 Barcelona, Spain. joaquim.segales@uab.es
authors.forename: J
authors.initials: J
authors.lastname: Segalés
authors.forename: F
authors.initials: F
authors.lastname: Alonso
authors.forename: C
authors.initials: C
authors.lastname: Rosell
authors.forename: J
authors.initials: J
authors.lastname: Pastor
authors.forename: F
authors.initials: F
authors.lastname: Chianini
authors.forename: E
authors.initials: E
authors.lastname: Campos
authors.forename: L
authors.initials: L
authors.lastname: López-Fuertes
authors.forename: J
authors.initials: J
authors.lastname: Quintana
authors.forename: G
authors.initials: G
authors.lastname: Rodríguez-Arrioja
authors.forename: M
authors.initials: M
authors.lastname: Calsamiglia
authors.forename: J
authors.initials: J
authors.lastname: Pujols
authors.forename: J
authors.initials: J
authors.lastname: Domínguez
authors.forename: M
authors.initials: M
authors.lastname: Domingo
mesh_terms: D000818:Animals; D018173:Circoviridae Infections / Q000276:immunology / Q000662:veterinary*; D005434:Flow Cytometry; D007075:Immunoglobulin M / Q000032:analysis; D007958:Leukocyte Count; D013552:Swine; D013553:Swine Diseases / Q000276:immunology*; D019282:Wasting Syndrome / Q000276:immunology / Q000662:veterinary*; D014886:Weaning
chemical_list: D007075:Immunoglobulin M