Expression of cyclin A in human leukemia cell lines HL-60 and K-562 at the level of light and electron microscope by using immunocytochemical methods.
In this study subcellular localization of cyclin A in the human promyelocytic leukemia cell line HL-60 and the human erythroleukemic K-562 cell line was examined by immunocytochemical methods. Studies were based on light and electron microscope evaluations. Cyclin A at the level of light microscope was present in 48% of the cells in HL-60 cell line and in 40% of the cells in K-562 line. Streptavidin-gold method was used for localization of cyclin A at the ultrastructural level. There was expression of cyclin A in the nucleus and in the cytoplasm. In the nucleus gold particles were seen to be associated with the condensed chromatin of the both leukemia cell lines. In the cytoplasm cyclin A was concentrated at a low level and was associated with ribosomes. Controls of the leukemia cells incubated with normal mouse serum showed no labeling at the light and electron microscope level.
Grzanka
A
A
Department of Clinical Pathomorphology, University School of Medical Sciences, Bydgoszcz, Poland. grzanka@wsp.bydgoszcz.pl
Styczynski
J
J
D000818:Animals; D002467:Cell Nucleus / Q000648:ultrastructure; D002843:Chromatin / Q000648:ultrastructure; D019925:Cyclin A / Q000032:analysis*; D003593:Cytoplasm / Q000648:ultrastructure; D018922:HL-60 Cells; D006801:Humans; D007150:Immunohistochemistry; D020014:K562 Cells; D004915:Leukemia, Erythroblastic, Acute; D015473:Leukemia, Promyelocytic, Acute; D051379:Mice; D016253:Microscopy, Immunoelectron
D002843:Chromatin; D019925:Cyclin A