Two-dimensional gel electrophoresis of nuclear proteins of different stages of B-chronic lymphocytic leukaemia.
Two-dimensional polyacrylamide gel electrophoresis was used to compare the composition of nuclear proteins from normal and B-chronic lymphocytic leukaemia (B-CLL) mononuclear cells. Some differences in the electrophoretic behaviour of these proteins from normal and transformed cells, especially with molecular weights/pI of 14-16 kD/5.9-7.4; 28-32 kD/4.9-5.5; 38-39 kD/5.4-6.1; 44-46 kD/5.1-5.6; 47-52 kD/5.0-5.6; 64-69 kD/5.1-5.7, and 95-105 kD/5.2-5.5, were observed. The comparative analysis of nuclear proteins, obtained from mononuclear cells of patients with B-CLL at different stages of development, indicated that the expression of some protein components might be correlated with the progression of this disease.
Rogalińska
M
M
Department of Cytobiochemistry, University of Lódź, Banacha, Poland.
Błoński
J
J
Robak
T
T
Kiliańska
Z M
ZM
D000368:Aged; D000369:Aged, 80 and over / Q000502:physiology; D018450:Disease Progression; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000378:metabolism* / Q000473:pathology*; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008875:Middle Aged; D008970:Molecular Weight; D009367:Neoplasm Staging; D009687:Nuclear Proteins / Q000032:analysis* / Q000302:isolation & purification; D012016:Reference Values
D009687:Nuclear Proteins