[Effects of hyaluronic acid-stimulating factor on viability and collagen synthesis of fibroblasts].
OBJECTIVE
To study the effects of hyaluronic acid-stimulating factor(HASF) on viability and collagen synthesis of fibroblasts derived from human dermis and scars.


METHODS
HASF was purified from amniotic fluid and fetal serum of gestational rabbits, and its activity was determined. To observe the effects of HASF on viability and collagen synthesis of two cell lines, AgNORs staining, 3H-proline incorporation and HYP determination were carried out.


RESULTS
HASF reduces AgNORs contents in skin FB and scar FBi 3H-proline incorporation and HYP contents were lower in experimental groups than those in controls in both cell lines. Two methods to detect collagen correlate well with each other.


CONCLUSIONS
HASF inhabits viability and collagen synthesis of both cell lines, thus may interfered with the formation of scar during wound healing.
Wang
Q
Q
Plastic Surgery Genter, Xijing Hospital, Xi'an 710032.
Lu
K
K
Yang
L
L
D000328:Adult; D000653:Amniotic Fluid / Q000737:chemistry; D001685:Biological Factors / Q000302:isolation & purification / Q000494:pharmacology*; D002478:Cells, Cultured; D002921:Cicatrix / Q000473:pathology*; D003094:Collagen / Q000096:biosynthesis*; D005260:Female; D005347:Fibroblasts / Q000378:metabolism*; D006801:Humans; D006820:Hyaluronic Acid / Q000378:metabolism*; D008297:Male; D016622:Silver Staining; D012867:Skin / Q000166:cytology / Q000378:metabolism*
D001685:Biological Factors; D006820:Hyaluronic Acid; D003094:Collagen