A novel method of isolation of a bone-morphogenetic-protein-like protein from ossein.
A new bone-morphogenetic-protein (BMP)-like protein has been isolated through a new protocol from a novel source, ossein. The BMP-like protein was hydrophilic and characterized through Fourier-transform IR studies, SDS/PAGE and coupled with a neutral binder, hydroxypropylmethylcellulose (HPMC) for control release. The IR spectrum of the protein showed peaks in tandem with BMP from bone matrix, and its molecular mass was in the range 18-21 kDa. Sustained release from the surface of HPMC was achieved for a period of 3 days.
Mythili
J
J
Department of Biochemistry, Central Leather Research Institute, Adyar Chennai 600020, India. mythly_21@yahoo.com
Padmavathy
S
S
Chandrakasan
G
G
D001671:Biochemistry / Q000379:methods; D001672:Biocompatible Materials / Q000737:chemistry*; D019485:Bone Morphogenetic Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D001842:Bone and Bones / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D065347:Hypromellose Derivatives; D008747:Methylcellulose / Q000031:analogs & derivatives / Q000737:chemistry; D011485:Protein Binding; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D013997:Time Factors
D001672:Biocompatible Materials; D019485:Bone Morphogenetic Proteins; D065347:Hypromellose Derivatives; D008747:Methylcellulose