Evaluation of three methods to measure anti-Brucella IgM antibodies and interference of IgA in the interpretation of mercaptan-based tests.
The results of a dipstick assay for the detection of immunoglobulin M (IgM) to Brucella smooth lipopolysaccharide (S-LPS) correlated with those of an enzyme-linked immunosorbent assay (ELISA) for IgM and of the serum agglutination test (SAT) performed with and without dithiothreitol. Two sera which were dithiothreitol-sensitive and were dipstick negative were shown to contain specific IgA. The dipstick assay is recommended as a simple method for detecting specific IgM antibodies in acute-phase brucellosis patients.
Marrodan
T
T
*Departamento de Microbiología, Servicio de Microbiología Clínica, Universidad de Navarra, Pamplona Spain, †Servicio de Enfermedades Infecciosas, Hospital de Bellvitge, Barcelona, Spain, ‡Servicio de Microbiología, Hospital Virgen de la Victoria, Málaga, Spain and §Department of Biomedical Research, Royal Tropical Institute, Amsterdam, The Netherlands.
Nenova-Poliakova
R
R
*Departamento de Microbiología, Servicio de Microbiología Clínica, Universidad de Navarra, Pamplona Spain, †Servicio de Enfermedades Infecciosas, Hospital de Bellvitge, Barcelona, Spain, ‡Servicio de Microbiología, Hospital Virgen de la Victoria, Málaga, Spain and §Department of Biomedical Research, Royal Tropical Institute, Amsterdam, The Netherlands.
Rubio
M
M
*Departamento de Microbiología, Servicio de Microbiología Clínica, Universidad de Navarra, Pamplona Spain, †Servicio de Enfermedades Infecciosas, Hospital de Bellvitge, Barcelona, Spain, ‡Servicio de Microbiología, Hospital Virgen de la Victoria, Málaga, Spain and §Department of Biomedical Research, Royal Tropical Institute, Amsterdam, The Netherlands.
Ariza
J
J
*Departamento de Microbiología, Servicio de Microbiología Clínica, Universidad de Navarra, Pamplona Spain, †Servicio de Enfermedades Infecciosas, Hospital de Bellvitge, Barcelona, Spain, ‡Servicio de Microbiología, Hospital Virgen de la Victoria, Málaga, Spain and §Department of Biomedical Research, Royal Tropical Institute, Amsterdam, The Netherlands.
Clavijo
E
E
*Departamento de Microbiología, Servicio de Microbiología Clínica, Universidad de Navarra, Pamplona Spain, †Servicio de Enfermedades Infecciosas, Hospital de Bellvitge, Barcelona, Spain, ‡Servicio de Microbiología, Hospital Virgen de la Victoria, Málaga, Spain and §Department of Biomedical Research, Royal Tropical Institute, Amsterdam, The Netherlands.
Smits
H L
HL
*Departamento de Microbiología, Servicio de Microbiología Clínica, Universidad de Navarra, Pamplona Spain, †Servicio de Enfermedades Infecciosas, Hospital de Bellvitge, Barcelona, Spain, ‡Servicio de Microbiología, Hospital Virgen de la Victoria, Málaga, Spain and §Department of Biomedical Research, Royal Tropical Institute, Amsterdam, The Netherlands.
Diaz
R
R
*Departamento de Microbiología, Servicio de Microbiología Clínica, Universidad de Navarra, Pamplona Spain, †Servicio de Enfermedades Infecciosas, Hospital de Bellvitge, Barcelona, Spain, ‡Servicio de Microbiología, Hospital Virgen de la Victoria, Málaga, Spain and §Department of Biomedical Research, Royal Tropical Institute, Amsterdam, The Netherlands.
D000372:Agglutination Tests / Q000379:methods*; D000907:Antibodies, Bacterial / Q000032:analysis*; D002002:Brucella / Q000276:immunology*; D002006:Brucellosis / Q000175:diagnosis / Q000276:immunology; D004229:Dithiothreitol; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D007124:Immunoenzyme Techniques; D007070:Immunoglobulin A / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis*; D008070:Lipopolysaccharides / Q000276:immunology
D000907:Antibodies, Bacterial; D007070:Immunoglobulin A; D007075:Immunoglobulin M; D008070:Lipopolysaccharides; D004229:Dithiothreitol