Sudden termination of G-CSF injection leads to apoptosis of a large proportion of increased granulocytes.
The effectiveness of granulocyte colony-stimulating factor (G-CSF) for granulocytopenia has been widely recognized. However, although granulocyte counts rapidly increase after a few injection of G-CSF, a large proportion of the increased granulocytes disappear from peripheral blood within a few days following G-CSF withdrawal. Where do they go? In this report, the answer can be seen at a glance. Using MitoCapture and a CCD camera-equipped fluorescence microscope, we succeeded in demonstrating that G-CSF withdrawal induced loss of mitochondrial membrane potential, i.e., an early stage of apoptosis, in human peripheral granulocytes increased by G-CSF.
Ogawa
Y
Y
Department of Radiology, Kochi Medical School, Oko-cho, Nankoku-shi, Kochi-Prefecture 783-8505, Japan. ogaway/kms@kochi-ms.ac.jp
Kobayashi
T
T
Nishioka
A
A
Kariya
S
S
Garcia Del Saz
E
E
Seguchi
H
H
Yoshida
S
S
D000380:Agranulocytosis / Q000139:chemically induced / Q000188:drug therapy; D000972:Antineoplastic Agents, Phytogenic / Q000009:adverse effects / Q000627:therapeutic use; D017209:Apoptosis / Q000187:drug effects*; D001772:Blood Cell Count; D001782:Blood Donors; D001943:Breast Neoplasms / Q000188:drug therapy; D000077143:Docetaxel; D004305:Dose-Response Relationship, Drug; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000627:therapeutic use*; D006098:Granulocytes / Q000473:pathology*; D006801:Humans; D007279:Injections, Subcutaneous; D008564:Membrane Potentials / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects; D017239:Paclitaxel / Q000009:adverse effects / Q000031:analogs & derivatives / Q000627:therapeutic use; D011994:Recombinant Proteins; D043823:Taxoids
D000972:Antineoplastic Agents, Phytogenic; D011994:Recombinant Proteins; D043823:Taxoids; D016179:Granulocyte Colony-Stimulating Factor; D000077143:Docetaxel; D017239:Paclitaxel