Proliferation and regeneration of retrogradely labeled adult rat corticospinal neurons in culture.
These are the first studies to demonstrate that adult rat corticospinal tract (CST) neurons, which were identified by retrograde neuronal labeling, retain regenerative and proliferative potential. To determine if adult CST neurons undergo cell division, we tested if these retrogradely labeled cells synthesize DNA by adding BrdU to the cultures 24 h prior to fixation of the cells. The result shows that adult corticospinal tract neurons are capable of DNA synthesis, and our total cell counts with labeled cells counts further suggest that these cells undergo cell division.
Alexanian
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Department of Anatomy and Neurobiology, Colorado State University, Fort Collins, Colorado 80523, USA.
Nornes
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D000818:Animals; D001370:Axonal Transport; D001973:Bromodeoxyuridine; D002455:Cell Division / Q000502:physiology*; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D007150:Immunohistochemistry; D007700:Kinetics; D008297:Male; D009416:Nerve Regeneration / Q000502:physiology*; D016501:Neurites / Q000502:physiology; D009474:Neurons / Q000166:cytology* / Q000502:physiology*; D011712:Pyramidal Tracts / Q000166:cytology* / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
D004247:DNA; D001973:Bromodeoxyuridine