title: Effects of beta-casomorphins on neuronal survival in culture of embryonic chick dorsal root ganglion neurons.
abstract: We studied the effects of beta-casomorphins (beta-CMs, mu-acting opioid peptides from milk protein beta-casein) on survival of primary-cultured chick dorsal root ganglion neurons in the presence of nerve growth factor. Beta-CM-5 and beta-CM-7 had potent neuronal survival-promoting activities. Beta-CM-4 amide (morphiceptin) and des-Tyr'-beta-CM-7 also exhibited the similar promoting effects, although their effects were very weak. The promoting effect of beta-CM-5 was prevented by co-administration of naloxone, or pretreatment with pertussis toxin. These results suggest that the neuronal survival-promoting effects of beta-CMs might be mediated through opioid receptors coupled to G proteins.
authors.affiliation: Laboratory of Cell Biology, Osaka University of Pharmaceutical Sciences, Takatsuki, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Sakaguchi
authors.forename: T
authors.initials: T
authors.lastname: Fujimori
authors.forename: T
authors.initials: T
authors.lastname: Satoh
authors.forename: E
authors.initials: E
authors.lastname: Matsumura
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002470:Cell Survival / Q000187:drug effects*; D002478:Cells, Cultured; D002642:Chick Embryo; D004723:Endorphins / Q000494:pharmacology*; D005727:Ganglia, Spinal / Q000166:cytology / Q000187:drug effects*; D009474:Neurons / Q000166:cytology / Q000187:drug effects*
chemical_list: D004723:Endorphins; C021968:beta-casomorphins