[Effects of exogenous factors on the activity of enzymes involved in carbon metabolism in thermoacidophilic bacteria of the genus Sulfobacillus].
Aerobic thermoacidophilic chemolithotrophic bacteria Sulfobacillus thermosulfidooxidans 1269T and Sulfobacillus thermosulfidooxidans subsp. asporogenes 41 were shown to be resistant to stress factors, including high concentrations of Zn2+ (0.8 M) and H+ (pH 1.2) that exceeded the optimum values. The growth and biomass gain rates decreased, but bacteria retained their functions. The activity of nearly all enzymes involved in carbon metabolism decreased. Glucose was primarily metabolized via the Entner--Doudoroff pathway. The activity tricarboxylic acid cycle enzymes decreased compared to that in cells grown under normal conditions. After saturation of the growth medium with 5 vol % CO2, sulfobacteria utilized glucose by the Embden-Meyerhof and pentose phosphate pathways under mixotrophic conditions.
Krasil'nikova
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EN
M. V. Lomonosov, Moscow State University, Moscow, 117312 Russia.
Tsaplina
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IA
Zakharchuk
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LM
Bogdanova
T I
TI
D001426:Bacterial Proteins / Q000378:metabolism; D002244:Carbon / Q000378:metabolism*; D002245:Carbon Dioxide / Q000378:metabolism; D004798:Enzymes / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D006091:Gram-Negative Chemolithotrophic Bacteria / Q000201:enzymology*; D006859:Hydrogen / Q000378:metabolism; D015032:Zinc / Q000378:metabolism
D001426:Bacterial Proteins; D004798:Enzymes; D002245:Carbon Dioxide; D002244:Carbon; D006859:Hydrogen; D005947:Glucose; D015032:Zinc
Vliianie ékzogennykh faktorov na aktivnost' fermentov metabolizma ugleroda u termoatsidofil'nykh bakteriĭ roda Sulfobacillus.