The renal Na-HCO3-cotransporter expressed in Xenopus laevis oocytes: change in stoichiometry in response to elevation of cytosolic Ca2+ concentration.
The Na+-HCO3- cotransporter of rat kidney (rkNBC) was expressed in Xenopus laevis oocytes to test whether cytosolic Ca2+ ([Ca2+]i) affects the cotransport stoichiometry. The current/voltage relationship of giant inside-out membrane patches of rkNBC-expressing oocytes was measured at near-physiological Na+ and HCO3- concentrations and the cotransport current, INBC, was defined as the current inhibited by 0.25 mmol/l tenidap. Essentially, we determined the reversal potential (VI=0) of INBC and the slope conductance (gNBC). The coupling ratio of (HCO3-) to Na+ (q) was calculated from VI=0. As reported in the preceding publication [Ducoudret et al., Pflügers Arch (2001) DOI 10.1007/s004240100594], in Ca2+-free solutions q was 2:1. This did not change when [Ca2+]i was increased to 0.1 micromol/l. At 0.5 micromol/l, however, only a few patches showed q=2:1, while most patches exhibited q=3:1. This indicates that [Ca2+]i affected the transport function of membrane-resident rkNBC molecules, and the bimodal distribution of VI=0 points to an indirect effect possibly mediated by differently expressed Ca2+-dependent protein kinases. The shift in q was associated with the predicted near twofold increase in gNBC and was confirmed by measurements of VI=0 at different Na+ and HCO3- concentrations. Because we previously observed that the cotransport in proximal tubule cells is susceptible to carbonic anhydrase (CA) inhibition, but only if it works at q=3:1, we propose that kNBC has three transport sites: when working at q=2:1 it binds 2 (HCO3-)+1 Na+, and while at q=3:1 it binds 1 CO3(2-)+1 HCO3- +1 Na+. The latter is equivalent to the transfer of 3 (HCO3-) +1 Na+, because in the presence of CA the generation of 1 CO3(2-) on one side of the membrane and its disintegration on the other transiently liberates 1 CO2, which follows by diffusion. This model explains the increase in (HCO3-) transport that is associated with the change in q from 2:1 to 3:1 by a selectivity change of a binding site from HCO3- to CO3(2-). This is more likely than the induction of a new transport pouch for a third (HCO3-) ion, which would require exceedingly large conformational changes of the transport protein.
Müller-Berger
S
S
Institut für Physiologische Chemie, Tierärztliche Hochschule, Bünteweg 17, 30559 Hannover, Germany.
Ducoudret
O
O
Diakov
A
A
Frömter
E
E
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D016861:Cyclooxygenase Inhibitors / Q000494:pharmacology; D007211:Indoles / Q000494:pharmacology; D007668:Kidney / Q000378:metabolism*; D008962:Models, Theoretical; D009865:Oocytes / Q000187:drug effects / Q000502:physiology*; D000078183:Oxindoles; D018408:Patch-Clamp Techniques; D051381:Rats; D011994:Recombinant Proteins / Q000378:metabolism; D027982:Sodium-Bicarbonate Symporters / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D013223:Statistics as Topic; D014982:Xenopus laevis
D016861:Cyclooxygenase Inhibitors; D007211:Indoles; D000078183:Oxindoles; D011994:Recombinant Proteins; D027982:Sodium-Bicarbonate Symporters; C062866:tenidap; D002118:Calcium