title: Modulation of immunostimulatory activity of CpG oligonucleotides by site-specific deletion of nucleobases.
abstract: The effect of nucleobase deletion in the 3'- or the 5'-flanking sequence to a CpG-motif on immunostimulatory activity of CpG-containing oligonucleotides was examined by cell proliferation, secretion of IL-12 and IL-6 in mouse spleen cell cultures, and by spleen enlargement in mice. Deletion of one or two nucleobases in the 3'-flanking sequence to a CpG-motif at certain positions did not affect immunostimulatory activity, while similar deletions in the 5'-flanking sequence increased immunostimulatory activity compared with the parent oligo.
authors.affiliation: Hybridon, Inc., 345 Vassar Street, Cambridge, MA 02139, USA.
authors.forename: D
authors.initials: D
authors.lastname: Yu
authors.forename: E R
authors.initials: ER
authors.lastname: Kandimalla
authors.forename: Q
authors.initials: Q
authors.lastname: Zhao
authors.forename: Y
authors.initials: Y
authors.lastname: Cong
authors.forename: S
authors.initials: S
authors.lastname: Agrawal
mesh_terms: D024509:3' Flanking Region; D024506:5' Flanking Region; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D018899:CpG Islands / Q000276:immunology*; D007274:Injections, Intraperitoneal; D051379:Mice; D008807:Mice, Inbred BALB C; D009841:Oligonucleotides / Q000737:chemistry / Q000276:immunology* / Q000494:pharmacology*; D009929:Organ Size / Q000187:drug effects; D017384:Sequence Deletion; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D013329:Structure-Activity Relationship
chemical_list: D009841:Oligonucleotides