Clinical significance of oral urease in diagnosis of Helicobacter pylori infection by [13C]urea breath test.
This study was performed to evaluate the effect of oral flora on [13C]urea breath test in detecting H. pylori infection and find an optimal method and timing for sample collection. Forty-five volunteers were included in this study. The [13C]urea breath test was performed using mouthwash, endoscopic administration, and conventional methods. According to the receiver-operating characteristic curves, the earliest optimal time for discriminating H. pylori-positive and H. pylori-negative patients was at 25 min with the mouthwash method with 78% sensitivity and 82% specificity, at 2 min with the endoscopic administration method with 100% sensitivity and 100% specificity, and at 6 min with the conventional method with 100% sensitivity and 95% specificity. The study shows a significant effect of oral urease on the results of the [13C]urea breath test. The timing of sampling collection can be shortened to 6 min with the conventional method or to 2 min through endoscopic administration.
Peng
N J
NJ
Department of Nuclear Medicine, Kaohsiung Veterans General Hospital, Taiwan.
Lai
K H
KH
Liu
R S
RS
Lee
S C
SC
Tsay
D G
DG
Lo
C C
CC
Tseng
H H
HH
Huang
W K
WK
Lo
G H
GH
Hsu
P I
PI
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001944:Breath Tests / Q000379:methods; D002247:Carbon Isotopes; D005260:Female; D005773:Gastroscopy; D016481:Helicobacter Infections / Q000175:diagnosis* / Q000382:microbiology; D016480:Helicobacter pylori / Q000201:enzymology; D006801:Humans; D008297:Male; D008875:Middle Aged; D009055:Mouth / Q000201:enzymology / Q000382:microbiology*; D009067:Mouthwashes; D012372:ROC Curve; D012680:Sensitivity and Specificity; D014510:Urease / Q000032:analysis*
D002247:Carbon Isotopes; D009067:Mouthwashes; D014510:Urease