Investigation of genotoxic and antigenotoxic activities of chlorophylls and chlorophyllin in cultured V79 cells.
Chlorophyll and its derivatives are examples of plant compounds (purified and/or extracted) which appear to protect DNA from damage caused by chemical or physical agents, although some studies have identified clastogenic activity of these compounds. This study was carried out to assess the genotoxic activity of chlorophyll-a (Chl-a), -b (Chl-b) and chlorophyllin (Chl) and their antigenotoxic activity against the DNA damage induced by methyl methanesulphonate (MMS) under conditions of simultaneous, pre-, post-treatment, and simultaneous treatment after pre-incubation of the chemical with MMS. The micronucleus (MN) test was used in binucleated cells (induced by cytochalasin-B) of a mammalian cell line (V79). The three concentrations of Chl-a, Chl-b or Chl (0.1375, 0.275, 0.55microM) were not genotoxic and the genotoxic action of MMS (400microM) decreased (74-117%) under all treatment conditions. The results showed that there was no significant difference among the treatment types, the concentration or the nature of chlorophyll used. The data obtained suggest that Chl-a, Chl-b and Chl when associated with the DNA damaging agent, MMS, may protect the DNA by desgenotoxic action and/or by bio-antigenotoxic mechanisms, with the similar efficiency.
Bez
G C
GC
Departamento de Biologia Geral, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Londrina, Paraná, Brazil.
Jordão
B Q
BQ
Vicentini
V E
VE
Mantovani
M S
MS
D000818:Animals; D016587:Antimutagenic Agents / Q000494:pharmacology*; D002460:Cell Line; D002734:Chlorophyll / Q000008:administration & dosage / Q000494:pharmacology* / Q000633:toxicity*; D000077194:Chlorophyll A; D002735:Chlorophyllides / Q000008:administration & dosage / Q000494:pharmacology* / Q000633:toxicity*; D006224:Cricetinae; D004249:DNA Damage; D008741:Methyl Methanesulfonate / Q000037:antagonists & inhibitors / Q000633:toxicity; D015162:Micronucleus Tests; D009153:Mutagens / Q000633:toxicity*
D016587:Antimutagenic Agents; D002735:Chlorophyllides; D009153:Mutagens; D002734:Chlorophyll; C037184:chlorophyll b; D008741:Methyl Methanesulfonate; C007020:chlorophyllin; D000077194:Chlorophyll A