title: Induction of cytokine mRNA in peripheral blood mononuclear cells of infants after the first dose of measles vaccine.
abstract: To better characterize the cytokine response to measles virus vaccine, we examined the levels of IL-2, IL-4, IL-5, IL-10, IL-12 and gamma-interferon (gamma-IFN) in measles virus-stimulated peripheral blood mononuclear cells from 18 donors before and 2 weeks after vaccination. Donors were grouped as seropositive or seronegative on the basis of measles-specific IgM antibody present at 2 weeks postvaccination. After vaccination, similar levels of upregulation of IL-2 and gamma-IFN mRNA were observed in the two groups. The majority of donors in both groups did not exhibit an increase in measles specific IL-4 or IL-10 mRNA after vaccination. IL-12 mRNA was not induced by measles virus in any of the donors. A statistically significant upregulation of IL-5 mRNA was observed among seropositive (9/13) compared with seronegative (1/5) donors after vaccination (P=0.09, one tailed Fisher's test). The observed measles specific induction of IL-5 mRNA is suggestive of a possible association between IL-5 production and an antibody response to measles virus.
authors.affiliation: Respiratory and Enteric Viruses Branch, Measles Section MS. D11, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30333, USA. hb11@cdc.gov
authors.forename: H
authors.initials: H
authors.lastname: Li
authors.forename: C J
authors.initials: CJ
authors.lastname: Hickman
authors.forename: R F
authors.initials: RF
authors.lastname: Helfand
authors.forename: H
authors.initials: H
authors.lastname: Keyserling
authors.forename: L J
authors.initials: LJ
authors.lastname: Anderson
authors.forename: W J
authors.initials: WJ
authors.lastname: Bellini
mesh_terms: D000914:Antibodies, Viral / Q000096:biosynthesis / Q000097:blood; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D007111:Immunity, Cellular; D007075:Immunoglobulin M / Q000096:biosynthesis / Q000097:blood; D007223:Infant; D007371:Interferon-gamma / Q000096:biosynthesis / Q000235:genetics*; D016753:Interleukin-10 / Q000096:biosynthesis / Q000235:genetics; D018664:Interleukin-12 / Q000096:biosynthesis / Q000235:genetics; D007376:Interleukin-2 / Q000096:biosynthesis / Q000235:genetics; D015847:Interleukin-4 / Q000096:biosynthesis / Q000235:genetics; D015848:Interleukin-5 / Q000096:biosynthesis / Q000235:genetics; D007378:Interleukins / Q000096:biosynthesis / Q000235:genetics*; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000378:metabolism*; D008213:Lymphocyte Activation / Q000235:genetics / Q000276:immunology*; D008297:Male; D008458:Measles Vaccine / Q000276:immunology*; D008459:Measles virus / Q000276:immunology*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis*; D014611:Vaccination
chemical_list: D000914:Antibodies, Viral; D007075:Immunoglobulin M; D007376:Interleukin-2; D015848:Interleukin-5; D007378:Interleukins; D008458:Measles Vaccine; D012333:RNA, Messenger; D016753:Interleukin-10; D018664:Interleukin-12; D015847:Interleukin-4; D007371:Interferon-gamma