title: Feline leukemia virus DNA vaccine efficacy is enhanced by coadministration with interleukin-12 (IL-12) and IL-18 expression vectors.
abstract: The expectation that cell-mediated immunity is important in the control of feline leukemia virus (FeLV) infection led us to test a DNA vaccine administered alone or with cytokines that favored the development of a Th1 immune response. The vaccine consisted of two plasmids, one expressing the gag/pol genes and the other expressing the env gene of FeLV-A/Glasgow-1. The genetic adjuvants were plasmids encoding the feline cytokines interleukin-12 (IL-12), IL-18, or gamma interferon (IFN-gamma). Kittens were immunized by three intramuscular inoculations of the FeLV DNA vaccine alone or in combination with plasmids expressing IFN-gamma, IL-12, or both IL-12 and IL-18. Control kittens were inoculated with empty plasmid. Following immunization, anti-FeLV antibodies were not detected in any kitten. Three weeks after the final immunization, the kittens were challenged by the intraperitoneal inoculation of FeLV-A/Glasgow-1 and were then monitored for a further 15 weeks for the presence of virus in plasma and, at the end of the trial, for latent virus in bone marrow. The vaccine consisting of FeLV DNA with the IL-12 and IL-18 genes conferred significant immunity, protecting completely against transient and persistent viremia, and in five of six kittens protecting against latent infection. None of the other vaccines provided significant protection.
authors.affiliation: Department of Veterinary Pathology, University of Glasgow, Bearsden, Glasgow G61 1QH, United Kingdom. lh40w@udcf.gla.ac.uk
authors.forename: L
authors.initials: L
authors.lastname: Hanlon
authors.forename: D
authors.initials: D
authors.lastname: Argyle
authors.forename: D
authors.initials: D
authors.lastname: Bain
authors.forename: L
authors.initials: L
authors.lastname: Nicolson
authors.forename: S
authors.initials: S
authors.lastname: Dunham
authors.forename: M C
authors.initials: MC
authors.lastname: Golder
authors.forename: M
authors.initials: M
authors.lastname: McDonald
authors.forename: C
authors.initials: C
authors.lastname: McGillivray
authors.forename: O
authors.initials: O
authors.lastname: Jarrett
authors.forename: J C
authors.initials: JC
authors.lastname: Neil
authors.forename: D E
authors.initials: DE
authors.lastname: Onions
mesh_terms: D000276:Adjuvants, Immunologic; D000818:Animals; D002415:Cats; D002460:Cell Line; D004279:DNA, Viral / Q000276:immunology*; D015685:Fusion Proteins, gag-pol / Q000235:genetics / Q000276:immunology*; D015870:Gene Expression; D005822:Genetic Vectors; D006801:Humans; D018664:Interleukin-12 / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D020382:Interleukin-18 / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D005256:Leukemia Virus, Feline / Q000235:genetics / Q000276:immunology*; D011995:Recombination, Genetic; D012192:Retroviridae Infections / Q000517:prevention & control*; D014412:Tumor Virus Infections / Q000517:prevention & control*; D014611:Vaccination; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000276:immunology*; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology*; D014771:Virion / Q000502:physiology; D019065:Virus Assembly / Q000502:physiology; D017735:Virus Latency
chemical_list: D000276:Adjuvants, Immunologic; D004279:DNA, Viral; D015685:Fusion Proteins, gag-pol; D020382:Interleukin-18; D019444:Vaccines, DNA; D014765:Viral Vaccines; D018664:Interleukin-12