Impaired synthesis of DHA in patients with X-linked retinitis pigmentosa.
Many patients with X-linked retinitis pigmentosa (XLRP) have lower than normal blood levels of the long-chain polyunsaturated omega3 fatty acid docosahexaenoic acid (DHA; 22:6omega3). This clinical trial was designed to test whether down-regulation of DHA biosynthesis might be responsible for these reduced DHA levels. DHA biosynthesis was assessed in five severely affected patients with XLRP and in five age-matched controls by quantifying conversion of [U-(13)C]alpha-linolenic acid (alpha-LNA) to [(13)C]DHA. Following oral administration of [U-(13)C]alpha-LNA, blood samples were collected at designated intervals for 21 days and isotopic enrichment of all omega3 fatty acids was determined by gas chromatography/mass spectroscopy. Activity of each metabolic step in the conversion of alpha-LNA to DHA was determined by comparison of the ratios of the integrated concentration of (13)C-product to (13)C-precursor in plasma total lipid fractions. The ratio of [(13)C]DHA to [(13)C]18:3omega3 (the entire pathway) and that of [(13)C]20:5omega3 to [(13)C]20:4omega3 (Delta(5)-desaturase) were significantly lower in patients versus controls (P = 0.03 and 0.05, respectively). The estimated biosynthetic rates of [(13)C]20:5omega3, [(13)C]22:5omega3, [(13)C]24:5omega3, [(13)C]24:6omega3, and [(13)C]22:6omega3 were significantly lower in XLRP patients (42%, 43%, 31%, 18%, and 32% of control values, respectively; P < 0.04), supporting down-regulation of Delta(5)-desaturase in XLRP. The disappearance of (13)C-labeled fatty acids from plasma was not greater in XLRP patients compared with controls, suggesting that XLRP was not associated with increased rates of fatty acid oxidation or other routes of catabolism.Thus, despite individual variation among both patients and controls, the data are consistent with a lower rate of Delta(5)-desaturation, suggesting that decreased biosynthesis of DHA may contribute to lower blood levels of DHA in patients with XLRP.
Hoffman
D R
DR
Retina Foundation of the Southwest, 9900 North Central Expressway, Dallas, TX 75231, USA. dhoffman@retinafoundation.org
DeMar
J C
JC
Heird
W C
WC
Birch
D G
DG
Anderson
R E
RE
D000328:Adult; D001944:Breath Tests; D002245:Carbon Dioxide / Q000032:analysis; D002247:Carbon Isotopes; D000090943:Delta-5 Fatty Acid Desaturase; D004281:Docosahexaenoic Acids / Q000097:blood*; D044943:Fatty Acid Desaturases / Q000378:metabolism; D015525:Fatty Acids, Omega-3 / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D008040:Genetic Linkage; D006801:Humans; D007700:Kinetics; D008297:Male; D008875:Middle Aged; D010084:Oxidation-Reduction; D012174:Retinitis Pigmentosa / Q000097:blood* / Q000235:genetics; D014960:X Chromosome; D017962:alpha-Linolenic Acid / Q000378:metabolism
D002247:Carbon Isotopes; D000090943:Delta-5 Fatty Acid Desaturase; D015525:Fatty Acids, Omega-3; D017962:alpha-Linolenic Acid; D002245:Carbon Dioxide; D004281:Docosahexaenoic Acids; D044943:Fatty Acid Desaturases