[Ca2+](i) rise in Jurkat E6-1 cell lines from different sources as a response to 50 Hz magnetic field exposure as a reproducible effect and independent of poly-L-lysine treatment.
Jurkat E6-1 cells obtained from three different sources were compared with respect to intracellular calcium response to a 50 Hz, 0.15 mT, magnetic field, to treatment with poly-L-lysine and to protein expression at the cell surface. The fura-2 single cell measurements were a replication study performed by three members of our group. The cells responded to the applied magnetic fields, although the percentage of responding cells was lower than in earlier studies. The geomagnetic field was backed off without changing the outcome of the intracellular calcium measurements. Fluorometric analyses showed no difference between the E6-1 cells obtained from three sources with respect to the expression of cell surface marker molecules. The addition of the cell adhesive peptide, poly-L-lysine, did not itself cause any effects on the intracellular calcium concentration.
Mattsson
M O
MO
Department of Natural Sciences, Orebro University, Orebro, Sweden.
Lindström
E
E
Still
M
M
Lindström
P
P
Mild
K H
KH
Lundgren
E
E
D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D002118:Calcium / Q000378:metabolism*; D004574:Electromagnetic Fields; D005456:Fluorescent Dyes / Q000737:chemistry; D005470:Fluorometry; D016257:Fura-2 / Q000737:chemistry; D006801:Humans; D019169:Jurkat Cells; D011107:Polylysine / Q000494:pharmacology; D015203:Reproducibility of Results; D013601:T-Lymphocytes / Q000187:drug effects / Q000378:metabolism*
D000945:Antigens, Differentiation, T-Lymphocyte; D005456:Fluorescent Dyes; D011107:Polylysine; D002118:Calcium; D016257:Fura-2