[The expression of HCV RNA polymerase in E.coli and the study of its solubility and antigenicity].
OBJECTIVE
To express HCV RNA polymerase and study its soluble condition and antigenicity.


METHODS
We constructed expression vectors pQE-5B-Fl and pQE-5B-C21 and expressed them in E.coli (M15). We analyzed their solubility in different conditions and purified soluble pQE-5B-C21 protein by Ni-NTA column, then detected its antigenicity by ELISA and western blot.


RESULTS
We obtained the purified soluble pQE-5B-C21 protein in the induction conditions of 18 degrees C and the protein was proved to be of good antigenicity by ELISA and western blot.


CONCLUSIONS
The RNA polymerase of HCV expressed in E.coli has good solubility and antigenicity.
Hou
L
L
Department of Applied Molecular Biology, Institute of Microbiology and Epidemiology, Beijing 100071, China.
Du
G
G
Lai
D
D
Wang
H
H
D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000235:genetics*; D006801:Humans; D012995:Solubility; D017361:Viral Nonstructural Proteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D012324:RNA-Dependent RNA Polymerase
D017361:Viral Nonstructural Proteins; C087036:NS-5 protein, hepatitis C virus; D012324:RNA-Dependent RNA Polymerase