title: Effect of the vacuolation of Helicobacter pylori.
abstract: Cytotoxic test in vitro combined with cytochemical stain, fluorescent stain, transmission electronmicrograph was used to study the vacuolated effect by helicobacter pylori (H. pylori) (Toxin+) and its pathological mechanism. 78.26% patients with peptic ulcer associated with H. pylori was infected with H. pylori (Toxin+), while 42.86% patients with gastritis was infected with H. pylori (Toxin+). It was positive in vacuole with acridine orange and acid phosphatase stain. Transmission electronmicrograph of vacuole revealed the presence of abounding membrane. There was a closed relationship between infection with H. pylori (Toxin+) and peptic ulcer disease. The vacuole induced by H. pylori (Toxin+) was autophagosome, which was pathological phenomenon induced by toxin.
authors.affiliation: Digestive Disease Institute of PLA, Nang Fang Hospital, Guangzhou 510515.
authors.forename: L
authors.initials: L
authors.lastname: Shi
authors.forename: X
authors.initials: X
authors.lastname: Hou
authors.forename: S
authors.initials: S
authors.lastname: Yi
authors.forename: J
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D001426:Bacterial Proteins / Q000633:toxicity*; D002522:Chlorocebus aethiops; D005260:Female; D005756:Gastritis / Q000382:microbiology; D016480:Helicobacter pylori / Q000302:isolation & purification / Q000472:pathogenicity*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010437:Peptic Ulcer / Q000382:microbiology; D014617:Vacuoles / Q000187:drug effects*; D014709:Vero Cells / Q000187:drug effects
chemical_list: D001426:Bacterial Proteins; C074689:VacA protein, Helicobacter pylori