Evaluation of polyphenol bioavailability in rat small intestine.
BACKGROUND
Dietary polyphenols, which are contained in several foods of plant origin, have been reported to be effective protective agents against cardiovascular diseases and cancer. However, data on their absorption from the gastrointestinal tract are still scarce and, often, contradictory.


OBJECTIVE
In this report, evaluation of polyphenol bioavailability was carried out by using segments of the small intestine from rat. The extent of absorption throughout the small intestine of rat was evaluated with two model compounds, tannic acid and catechin, as representatives of high and low molecular weight polyphenols, respectively. The consequence of the binding of tannic acid to BSA on both tannic acid absorption and in vivo protein digestibility was also examined.


METHODS
Polyphenol solutions of different concentrations were injected into the lumen of ligated segments (6 cm) of the small intestine and the segments incubated in buffer for 5 min. The residual amount of polyphenol in the lumen of each segment was assayed by maximum absorption in the UV/VIS optical spectrum as was the amount of compound that had crossed the gut wall into the incubation buffer. Digestibility of BSA and of a BSA- tannic acid complex was assayed with rats.


RESULTS
The results indicated a significant, concentration-dependent, disappearance of both polyphenols from the small intestine of the rat, with higher uptake levels being evident for tannic acid (50%) than catechin (30%). However, complete transfer through the gut wall was not observed with tannic acid whilst low but significant amounts (10%) were detected in incubation buffers with catechin. Partial binding of polyphenols by endogenous proteins in the intestinal lumen was also demonstrated. Complexing tannic acid with BSA (1:10 mol/mol) was not found to affect either the extent of interaction of tannic acid with the small intestine or the in vivo digestibility of the protein.


CONCLUSIONS
Our experiments show that tannic acid and catechin both interact with the gut but only catechin appears able to traverse the gut. In addition, they provide evidence for binding of tannic acid and catechin by endogenous proteins in the intestinal lumen. This may limit their absorption from the small intestine. BSA complexed with tannic acid was as readily digested as BSA alone. This may suggest that tannic acid exerts anti-nutritional effects by binding to proteins of the gut wall and interfering with gut function rather than by inhibition of dietary protein digestion.
Carbonaro
M
M
Istituto Nazionale di Ricerca per gli Alimenti e la Nutrizione, Roma, Italy. carbonaro@inran.it
Grant
G
G
Pusztai
A
A
D000818:Animals; D001682:Biological Availability; D002392:Catechin / Q000493:pharmacokinetics; D046508:Culture Techniques; D004044:Dietary Proteins / Q000378:metabolism*; D004063:Digestion; D004305:Dose-Response Relationship, Drug; D005419:Flavonoids; D047348:Hydrolyzable Tannins / Q000493:pharmacokinetics; D007408:Intestinal Absorption; D007421:Intestine, Small / Q000378:metabolism*; D023421:Models, Animal; D010636:Phenols / Q000493:pharmacokinetics*; D011108:Polymers / Q000493:pharmacokinetics*; D051381:Rats; D012710:Serum Albumin, Bovine / Q000378:metabolism
D004044:Dietary Proteins; D005419:Flavonoids; D047348:Hydrolyzable Tannins; D010636:Phenols; D011108:Polymers; D012710:Serum Albumin, Bovine; D002392:Catechin