Capability of arsonolipids to transport divalent cations: a Pressman cell study.
The ability of the newly synthesized arsonolipids (2,3-diacyloxyprophlarsonic acids) to transport cations was studied using the Pressman cell. Experimental results demonstrate that arsonolipids are much more efficient carriers of Ca(2+) and Mg(2+) than natural phosphatidic acid in the Pressman cell experiments. The ability of arsonolipids to transfer Ca(2+) is affected by the lipid side chain length in the order: C(12)>>C(14) approximately C(16). Ca(2+) is transferred faster than Mg(2+), suggesting that the latter is more tightly bound to the arsonolipids. The transfer kinetic curves are parabolic for C(12), while initially linear with a tendency to reach a steady state for C(14) and C(16), when the pH in the donor compartment was 8.3. The transport kinetics for both ions studied were best fitted by an equation derived from saturation kinetics that apply in reversible chemical reactions. The ion transfer rates increased as the pH in the donor compartment decreased.
Gortzi
O
O
Laboratoty of Pharmaceutical Technology, Department of Pharmacy, University of Patras, Rio 26500, Patras, Greece.
Klepetsanis
P
P
Antimisiaris
S G
SG
Ioannou
P V
PV
D001152:Arsenicals / Q000737:chemistry*; D002118:Calcium / Q000737:chemistry*; D002413:Cations, Divalent / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008055:Lipids / Q000737:chemistry*; D008274:Magnesium / Q000737:chemistry*; D008567:Membranes, Artificial; D008954:Models, Biological; D010712:Phosphatidic Acids / Q000737:chemistry; D013329:Structure-Activity Relationship
D001152:Arsenicals; D002413:Cations, Divalent; D008055:Lipids; D008567:Membranes, Artificial; D010712:Phosphatidic Acids; C054806:arsonic acid; D008274:Magnesium; D002118:Calcium