Direct amplification of length polymorphism analysis differentiates Panax ginseng from P. quinquefolius.
The method of direct amplification of length polymorphism (DALP) was applied to authenticate Panax ginseng and P. quinquefolius. A 636-bp DALP fragment was present in all P. ginseng but absent in all the P. quinquefolius cultivars examined. We have shown that the use of DALP and conversion of specific polymorphic band to sequence-tagged site (STS) for quick authentication may be applied to authenticate related medicinal materials.
Ha
W Y
WY
Yau
F C
FC
But
P P
PP
Wang
J
J
Shaw
P C
PC
D001483:Base Sequence; D018744:DNA, Plant; D004365:Drugs, Chinese Herbal; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques; D005894:Panax / Q000145:classification* / Q000235:genetics; D010936:Plant Extracts / Q000235:genetics; D018517:Plant Roots / Q000235:genetics; D011110:Polymorphism, Genetic; D016324:Sequence Tagged Sites; D013045:Species Specificity
D018744:DNA, Plant; D004365:Drugs, Chinese Herbal; D010936:Plant Extracts