Antioxidant and radical scavenging properties in vitro of polyphenolic extracts from red wine.
OBJECTIVE
Red wine polyphenols inhibit chemically-induced oxidative DNA damage in vivo in experimental animals through a mechanism which is still unclear. On this basis, we tried to clarify the mechanisms of inhibition of DNA oxidation in vitro by wine extracts containing monomeric and polymeric phenols (WE) and monomer-free complex polyphenols and tannins (WCPT) from red wine.


METHODS
Oxidative DNA damage was induced by incubating DNA with GSH/Fe3+ or cumene hydroperoxide (CumOOH) in vitro and using 8-OH-2-deoxyguanosine (8-OHdG) levels as a measure of DNA oxidation. Levels of 8-OHdG were determined by HPLC coupled with electrochemical detector (ESA).


CONCLUSIONS
WCPT and WE, at microM concentrations, reduced concentration-dependently oxidative DNA damage induced by GSH/Fe3+. WCPT and WE also reduced DNA oxidation by CumOOH. In conclusion, complex polyphenols and tannin extracts from red wine, with or without small molecular phenols, prevent oxidative DNA damage through a dual mechanism, iron binding and direct free radical scavenging.
Lodovici
M
M
Department of Pharmacology, University of Florence, Italy.
Guglielmi
F
F
Casalini
C
C
Meoni
M
M
Cheynier
V
V
Dolara
P
P
D000818:Animals; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D004249:DNA Damage / Q000187:drug effects*; D005399:Fishes; D005419:Flavonoids; D016166:Free Radical Scavengers / Q000378:metabolism*; D066298:In Vitro Techniques; D007501:Iron; D007502:Iron Chelating Agents / Q000494:pharmacology; D008297:Male; D010084:Oxidation-Reduction; D010636:Phenols / Q000378:metabolism / Q000494:pharmacology*; D011108:Polymers / Q000378:metabolism / Q000494:pharmacology*; D059808:Polyphenols; D012661:Semen; D013634:Tannins / Q000378:metabolism / Q000494:pharmacology*; D014920:Wine
D000975:Antioxidants; D005419:Flavonoids; D016166:Free Radical Scavengers; D007502:Iron Chelating Agents; D010636:Phenols; D011108:Polymers; D059808:Polyphenols; D013634:Tannins; D007501:Iron