Evaluation of the dietetic and therapeutic potential of a high molecular weight hydroxycinnamate-derived polymer from Symphytum asperum Lepech. Regarding its antioxidant, antilipoperoxidant, antiinflammatory, and cytotoxic properties.
A water-soluble hydroxycinnamate-derived polymer (>1000 kDa) from Symphytum asperum Lepech. (Boraginaceae) strongly reduced the diphenylpicrylhydrazyl radical (IC(50) approximately 0.7 microg/mL) and inhibited the nonenzymatic lipid peroxidation of bovine brain extracts (IC(50) approximately 10 ng). This polymer exhibited only a low hydroxyl radical scavenging effect in the Fe(3+)-EDTA-H(2)O(2) deoxyribose system (IC(50) > 100 microg/mL) but strongly decreased superoxide anion generation in either the reaction of phenazine methosulfate with NADH and molecular oxygen (IC(50) approximately 13.4 microg/mL) or in rat PMA-activated leukocytes (IC(50) approximately 5 microg/mL). The ability to inhibit both degranulation of azurophil granules and superoxide generation in primed leukocytes indicates that the NADPH oxidase responsible for this later effect is inhibited, pointing to the Symphytum asperum polymer as a potent antiinflammatory and vasoprotective agent. At all concentrations tested (0-200 microg/mL), we observed no cytotoxicity on normal human fibroblasts and neither antiproliferative effects nor proliferation activation on neoplastic cells.
Barthomeuf
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UMR-INSERM U-484, Laboratoire de Pharmacognosie et Biotechnologies, Université d'Auvergne, Faculté de Pharmacie, Place H. Dunant, 63001 Clermont-Fd Cedex, France. Chantal.Barthomeuf@u-clermont1.fr
Debiton
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Barbakadze
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Kemertelidze
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D000230:Adenocarcinoma / Q000188:drug therapy*; D000893:Anti-Inflammatory Agents / Q000494:pharmacology* / Q000627:therapeutic use; D005069:Evaluation Studies as Topic; D006801:Humans; D020128:Inhibitory Concentration 50; D015227:Lipid Peroxidation / Q000187:drug effects; D008970:Molecular Weight; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D018514:Plant Structures; D010946:Plants, Medicinal; D013481:Superoxides; D018675:Toxicity Tests; D014407:Tumor Cells, Cultured
D000893:Anti-Inflammatory Agents; D010936:Plant Extracts; D013481:Superoxides