title: Sequence analysis and insertional inactivation of a gene encoding Moraxella sp. CK-1 cell wall hydrolase.
abstract: Sequencing of the Moraxella sp. CK-1 autolysin (cell wall hydrolases) gene showed the presence of an open reading frame which encodes a polypeptide of 273 amino acids with a molecular mass of 33,316 Da. A presumed ribosomal binding site, a possible -10 and -35 region, and rho-dependent terminators were found. The C-terminal region of the mature protein showed considerable homology with the Thermus sp. serine proteinase. Enzyme assay suggests that the recombinant autolysin has amidase or endopeptidase activity. Analysis of the peptidoglycan fragments, following the treatment with the autolysin, indicates that this protein is an N-acetylmuramyl-L-alanine amidase. Insertional inactivation of the autolysin of Moraxella sp. CK-1 chromosome led to a decrease in cell wall hydrolytic activity, clumping of the cells, and color change. No lytic band present in inactivated magA mutant by renaturing SDS-PAGE.
authors.affiliation: Department of Biology, College of Natural Sciences, Hanyang University, Seoul, Korea.
authors.forename: H M
authors.initials: HM
authors.lastname: Kim
authors.forename: T B
authors.initials: TB
authors.lastname: Ohn
authors.forename: B R
authors.initials: BR
authors.lastname: Min
authors.forename: Y K
authors.initials: YK
authors.lastname: Choi
mesh_terms: D000595:Amino Acid Sequence; D001433:Bacteriolysis; D001483:Base Sequence; D002473:Cell Wall / Q000201:enzymology*; D008969:Molecular Sequence Data; D009016:Moraxella / Q000201:enzymology* / Q000235:genetics; D016254:Mutagenesis, Insertional; D009238:N-Acetylmuramoyl-L-alanine Amidase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D009238:N-Acetylmuramoyl-L-alanine Amidase