DNA transfection using linear poly(ethylenimine) prepared by controlled acid hydrolysis of poly(2-ethyl-2-oxazoline).
A series of linear poly(ethylenimine) (L-PEI) containing varying amounts of cationic charge density in its backbone was produced by controlled hydrolysis of poly(2-ethyl-2-oxazoline) (PEtOz) for using as a nonviral DNA transfection agent. The effects of cationic charge density and molecular weight of the L-PEI on the cytotoxicity and transfection efficiency were studied. The efficiency of transfection was monitored by using a luciferase reporter gene system. Gel retardation assay and dynamic light scattering (DLS) showed that the condensation capacity of L-PEI was suitable for transfection. Highly compacted L-PEI/DNA complex ( approximately 150 nm) was obtained with a surface charge value of around +28.4 mV. Cell cytotoxicity was affected to a great extent by the hydrolysis percent of L-PEI as well as by the molecular weight. Transfection efficiency of luciferase plasmid DNA against NIH 3T3 fibroblast was largely dependent upon the hydrolysis percent (charge density) in the polymer backbone and the molecular weight of the L-PEI, but independent of the total amount of cationic charges used for DNA condensation. L-PEI with a hydrolysis percent of 88.0% exhibited comparable transfection efficiency to that of commonly used branched PEI.
Jeong
J H
JH
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 305-701, Taejon, South Korea.
Song
S H
SH
Lim
D W
DW
Lee
H
H
Park
T G
TG
D016475:3T3 Cells; D000818:Animals; D004247:DNA / Q000008:administration & dosage*; D006868:Hydrolysis; D051379:Mice; D008970:Molecular Weight; D010080:Oxazoles / Q000737:chemistry*; D011094:Polyethyleneimine / Q000008:administration & dosage*; D011108:Polymers / Q000737:chemistry*; D014162:Transfection
D010080:Oxazoles; D011108:Polymers; C071857:polyethyloxazoline; D011094:Polyethyleneimine; D004247:DNA