Production in vitro, on different solid culture media, of two distinct exopolysaccharides by a mucoid clinical strain of Burkholderia cepacia.
The production of exopolysaccharides (EPSs) by a mucoid clinical isolate of Burkholderia cepacia involved in infections in cystic fibrosis patients, was studied. Depending on the growth conditions, this strain was able to produce two different EPS, namely PS-I and PS-II, either alone or together. PS-I is composed of equimolar amounts of glucose and galactose with pyruvate as substituent, and was produced on all media tested. PS-II is constituted of rhamnose, mannose, galactose, glucose and glucuronic acid in the ratio 1:1:3:1:1, with acetate as substituent, and was produced on either complex or minimal media with high-salt concentrations (0.3 or 0.5 M NaCl). Although this behavior is strain-specific, and not cepacia-specific, the stimulation of production of PS-II in conditions that mimic those encountered by B. cepacia in the respiratory track of cystic fibrosis patients, suggests a putative role of this EPS in a pathologic context.
Cérantola
S
S
Institut de Pharmacologie et de Biologie Structurale, UMR 9062, CNRS-UPS 205 route de Narbonne, F-31077, Toulouse, France.
Montrozier
H
H
D016956:Burkholderia cepacia / Q000254:growth & development / Q000378:metabolism*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D003470:Culture Media / Q000737:chemistry / Q000378:metabolism*; D003550:Cystic Fibrosis / Q000382:microbiology*; D005947:Glucose / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D019697:Onions / Q000382:microbiology; D010641:Phenotype; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis* / Q000737:chemistry; D013696:Temperature
D003470:Culture Media; D011135:Polysaccharides, Bacterial; D005947:Glucose; D005990:Glycerol