title: Localized gene-specific induction of accessibility to V(D)J recombination induced by E2A and early B cell factor in nonlymphoid cells.
abstract: Accessibility of immunoglobulin (Ig) gene segments to V(D)J recombination is highly regulated and is normally only achieved in B cell precursors. We previously showed that ectopic expression of E2A or early B cell factor (EBF) with recombination activating gene (RAG) induces rearrangement of IgH and IgL genes in nonlymphoid cells. VkappaI genes throughout the locus were induced to rearrange after transfection with E2A, suggesting that the entire Vkappa locus was accessible. However, here we show that Ig loci are not opened globally but that recombination is localized. Gene families are interspersed in the D(H), Vkappa, and Vlambda loci, and we show that certain families and individual genes undergo high levels of recombination after ectopic expression of E2A or EBF, while other families within the same locus are not induced to rearrange. Furthermore, in some families, induction of germline transcription correlates with the level of induced recombination, while in others there is no correlation, suggesting that recombination is not simply initiated by induction of germline transcription. The induced repertoire seen at 24 hours does not change significantly over time indicating the absence of many secondary rearrangements and also suggesting a direct targeting mechanism. We propose that accessibility occurs in a local manner, and that binding sites for factors facilitating accessibility are therefore likely to be associated with individual gene segments.
authors.affiliation: The Scripps Research Institute, Department of Immunology IMM-22, La Jolla, CA 92037, USA.
authors.forename: P
authors.initials: P
authors.lastname: Goebel
authors.forename: N
authors.initials: N
authors.lastname: Janney
authors.forename: J R
authors.initials: JR
authors.lastname: Valenzuela
authors.forename: W J
authors.initials: WJ
authors.lastname: Romanow
authors.forename: C
authors.initials: C
authors.lastname: Murre
authors.forename: A J
authors.initials: AJ
authors.lastname: Feeney
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D051792:Basic Helix-Loop-Helix Proteins; D002460:Cell Line; D004254:DNA Nucleotidyltransferases / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D015321:Gene Rearrangement; D005803:Genes, Immunoglobulin; D016654:Genes, RAG-1; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D007145:Immunoglobulin kappa-Chains / Q000235:genetics*; D005810:Multigene Family; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000378:metabolism; D011995:Recombination, Genetic; D015534:Trans-Activators / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism*; D014158:Transcription, Genetic; D014162:Transfection; D045567:VDJ Recombinases
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D004254:DNA Nucleotidyltransferases; D004268:DNA-Binding Proteins; D018398:Homeodomain Proteins; D007135:Immunoglobulin Variable Region; D007145:Immunoglobulin kappa-Chains; D011994:Recombinant Proteins; D015534:Trans-Activators; D014157:Transcription Factors; D045567:VDJ Recombinases; C081976:EBF1 protein, human; C407909:TCF3 protein, human; C064658:RAG-1 protein