title: Triglyceride content in skeletal muscle: variability and the source.
abstract: Intramyocellular triglycerides (imcTG) of skeletal muscle are an important energy source for muscle work in mammals. However, the metabolism and regulation of this small intracellular neutral lipid pool are largely unknown. This is in part due to the difficulties involved with its sampling and measurement introduced by contaminants of extramyocellular triglycerides (emcTG). The contents of imcTG reported to date for both human and rodent muscle are of unusually high variability not only across species, but also within same individuals and even same muscle groups. Recent studies suggested that the inherent muscle histologic heterogeneity does not appear to be a major source for the high variability as previously believed. Rather, the new experimental data showed that the procedures commonly used to process muscle specimens before lipid extraction appear inadequate to ensure a complete removal of emcTG contaminants. The extramyocellular lipid contaminants cause an overestimation of imcTG content and markedly increase its variability. Careful and meticulous microdissection has been found necessary in order to avoid contamination by emcTG, thereby obtaining pure muscle fibers for extraction of imcTG.
authors.affiliation: Endocrine Research Unit, Mayo Foundation, Rochester, Minnesota 55905, USA. guo.zengkui@mayo.edu
authors.forename: Z
authors.initials: Z
authors.lastname: Guo
mesh_terms: D017667:Adipocytes / Q000737:chemistry; D000818:Animals; D004210:Dissection / Q000379:methods; D006801:Humans; D008055:Lipids / Q000032:analysis* / Q000737:chemistry; D018485:Muscle Fibers, Skeletal / Q000737:chemistry* / Q000166:cytology; D018482:Muscle, Skeletal / Q000737:chemistry* / Q000166:cytology; D051381:Rats; D013048:Specimen Handling / Q000379:methods; D014280:Triglycerides / Q000032:analysis*
chemical_list: D008055:Lipids; D014280:Triglycerides