title: An analytical method for the rapid screening of organophosphate pesticides in human biological samples and foodstuffs.
abstract: Gas chromatography with nitrogen/phosphorus sensitive detection (GC/PND) and electron impact mass spectrometry (GC/MS) with selected ion monitoring provides a simple, rapid and sensitive method for the determination of organophosphate pesticides (OPs). A selective single-step extraction of 23 different OPs in urine, blood, serum and food samples (baby food, soft drinks and instant soups suspected of contamination from a blackmailing scare) is described. The OPs were extracted with 1ml toluene (with and without addition of mevinphos as internal standard), using a 0.7ml aliquot of urine, blood or serum sample. Food samples (0.2g) were homogenised with water (0.5ml) before extraction. An amount of 1microl of the toluene phase (extraction supernatant) was analysed directly by GC/PND and GC/MS.The method was validated using spiked human serum. The OPs were mixed with serum containing 10mg/ml disodium ethane diamine tetraacetic acid disodium salt (EDTA disodium salt) and stored up to 10 days at 4 and -20 degrees C, respectively. The recovery rates of OPs in freshly spiked human plasma ranged between 50% (dimethoate) and 133% (dialifos). OPs in plasma proved to be stable at -20 degrees C. Their levels decreased only slightly after storage at 4 degrees C.
authors.affiliation: Institute of Legal Medicine, Heinrich-Heine-University, P.O. Box 10 10 07, D-40001 Duesseldorf, Germany.
authors.forename: F A
authors.initials: FA
authors.lastname: Tarbah
authors.forename: H
authors.initials: H
authors.lastname: Mahler
authors.forename: O
authors.initials: O
authors.lastname: Temme
authors.forename: T
authors.initials: T
authors.lastname: Daldrup
mesh_terms: D000818:Animals; D005506:Food Contamination / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D007306:Insecticides / Q000032:analysis / Q000097:blood* / Q000652:urine; D009943:Organophosphorus Compounds; D051381:Rats
chemical_list: D007306:Insecticides; D009943:Organophosphorus Compounds