title: Detection of haemoparasites in cattle by reverse line blot hybridisation with a note on the distribution of ticks in Sicily.
abstract: A reverse line blot hybridisation (RLB) of 21 oligonucleotides with polymerase chain reaction (PCR) amplified regions of 16S rRNA (Ehrlichia/Anaplasma group) or 18S rRNA (Babesia/Theileria group) genes of haemoparasites detected Theileria annulata, T. buffeli/orientalis, Babesia bovis, B. bigemina, B. divergens, Ehrlichia bovis, Anaplasma marginale, A. centrale and unknown species within the Rickettsia tribe.A very high prevalence of mixed infections was detected, which indicated that animals infected with Babesia spp. were also infected with Theileria spp. and/or Anaplasma spp. The tick distribution appeared to be seasonal with Hyalomma marginatum as the most frequently observed tick and Boophilus annulatus and Ixodes ricinus as the least frequently observed ticks. Other species identified in the 818 ticks collected during the five sampling periods between April 1998 and November 1999 included H. lusitanicum, Rhipicephalus sanguineus group, R. bursa, Dermacentor marginatus, Haemaphysalis punctata, B. annulatus and I. ricinus.
authors.affiliation: Division of Parasitology and Tropical Veterinary Medicine, Faculty of Veterinary Medicine, Utrecht University, P.O. Box 80.165, 3508 TD Utrecht, The Netherlands.
authors.forename: K
authors.initials: K
authors.lastname: Georges
authors.forename: G R
authors.initials: GR
authors.lastname: Loria
authors.forename: S
authors.initials: S
authors.lastname: Riili
authors.forename: A
authors.initials: A
authors.lastname: Greco
authors.forename: S
authors.initials: S
authors.lastname: Caracappa
authors.forename: F
authors.initials: F
authors.lastname: Jongejan
authors.forename: O
authors.initials: O
authors.lastname: Sparagano
mesh_terms: D000709:Anaplasma / Q000235:genetics / Q000302:isolation & purification; D000712:Anaplasmosis / Q000175:diagnosis* / Q000453:epidemiology; D000818:Animals; D001096:Arachnid Vectors / Q000382:microbiology / Q000469:parasitology / Q000502:physiology; D001403:Babesia / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D001404:Babesiosis / Q000175:diagnosis / Q000453:epidemiology / Q000662:veterinary*; D001483:Base Sequence; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000453:epidemiology / Q000382:microbiology / Q000469:parasitology; D004536:Ehrlichia / Q000235:genetics / Q000302:isolation & purification; D016873:Ehrlichiosis / Q000175:diagnosis / Q000453:epidemiology / Q000662:veterinary*; D005784:Gene Amplification; D007558:Italy / Q000453:epidemiology; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D016794:Theileria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D013801:Theileriasis / Q000175:diagnosis* / Q000453:epidemiology; D017282:Tick-Borne Diseases / Q000175:diagnosis / Q000453:epidemiology / Q000662:veterinary; D013987:Ticks / Q000382:microbiology / Q000469:parasitology / Q000502:physiology
chemical_list: D012336:RNA, Ribosomal, 16S; D012337:RNA, Ribosomal, 18S