Identification and characterization of Carnobacteria isolated from fish intestine.
Eleven bacterial strains were isolated from the gastrointestinal tract of four fish species, Atlantic salmon (Salmo salar L.), Arctic charr (Salvelinus alpinus L.), Atlantic cod (Gadus morhua L.) and wolffish (Anarhichas lupus L.). All the strains were Gram-positive rods, non-sporing, catalase and oxidase-negative, able to grow at pH 9.0 but not on acetate containing media (pH < or = 5.4), and were fermentative. They had a high content of oleic acid (18:1 n-9) in cellular lipid, and were found to belong to the genus Carnobacterium by phenotypic criteria. The eleven carnobacteria strains were further identified on the basis of 16S rDNA sequence analysis and AFLP(TM) fingerprinting.
Ringø
E
E
Department of Arctic Veterinary Medicine, The Norwegian School of Veterinary Science, Tromsø. Einar.Ringo@veths.no
Wesmajervi
M S
MS
Bendiksen
H R
HR
Berg
A
A
Olsen
R E
RE
Johnsen
T
T
Mikkelsen
H
H
Seppola
M
M
Strøm
E
E
Holzapfel
W
W
D000333:Aeromonas / Q000254:growth & development / Q000472:pathogenicity; D000818:Animals; D000898:Antibiosis; D001483:Base Sequence; D050260:Carbohydrate Metabolism; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D004275:DNA, Ribosomal / Q000032:analysis / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005399:Fishes / Q000382:microbiology*; D007422:Intestines / Q000382:microbiology*; D007777:Lactobacillaceae / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification / Q000648:ultrastructure; D008969:Molecular Sequence Data; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D020317:Salmo salar / Q000382:microbiology; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014337:Trout / Q000382:microbiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S