Helicobacter pylori membrane protein 1: a new carcinogenic factor of Helicobacter pylori.
Considering a suspected link between Helicobacter pylori infection and human stomach cancer, a new H. pylori gene for membrane protein 1 (HP-MP1) was recently cloned. Because HP-MP1 induces release of inflammatory cytokines and tumor necrosis factor-alpha acts as both initiator and tumor promoter, we studied the possible involvement of HP-MP1 in carcinogenesis of H. pylori. Two cell lines, BALB/3T3 cells as control and v-Ha-ras-transfected BALB/3T3 cells (Bhas 42 cells) as putative initiated cells, were each transfected with HP-MP1, urease B genes, or vector alone. All of the Bhas/mpl clones showed strong expression of tumor necrosis factor-alpha gene and produced tumors in 100% of nude mice. Two Bhas/ure clones showed weak tumorigenicity; the other Bhas and BALB clones showed none. Results indicate strong carcinogenic activity of HP-MP1 in cooperation with viral Ras protein and weak activity of urease B.
Suganuma
M
M
Saitama Cancer Center, Saitama 362-0806, Japan.
Kurusu
M
M
Okabe
S
S
Sueoka
N
N
Yoshida
M
M
Wakatsuki
Y
Y
Fujiki
H
H
D016475:3T3 Cells; D000818:Animals; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000502:physiology*; D002471:Cell Transformation, Neoplastic / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics; D016481:Helicobacter Infections / Q000150:complications; D016480:Helicobacter pylori / Q000235:genetics / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D013274:Stomach Neoplasms / Q000382:microbiology; D014162:Transfection; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology; D014510:Urease / Q000235:genetics; D018631:ras Proteins / Q000235:genetics / Q000502:physiology
D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; C116708:HP-MP1 protein, Helicobacter pylori; D014409:Tumor Necrosis Factor-alpha; D014510:Urease; D018631:ras Proteins