Excitotoxicity of lathyrus sativus neurotoxin in leech retzius neurons.
The effects of Lathyrus sativus neurotoxin were studied on the cell membrane potential and cellular cation composition in Retzius nerve cells of the leech Haemopis sanguisuga, with ion-selective microelectrodes using liquid ion-exchangers. Bath application of 10(-4) mol/l Lathyrus sativus neurotoxin for 3 min depolarized the cell membrane potential and decreased the input resistance of directly polarized membrane in Retzius neurons. At the same time the cellular Na+ activity increased and cellular K+ activity decreased with slow but complete recovery, while the intracellular Ca2+ concentration was not changed. Na+-free Ringer solutions inhibited the depolarizing effect of the neurotoxin on the cell membrane potential. Zero-Ca2+ Ringer solution or Ni2+-Ringer solution had no influence on the depolarizing effect of the neurotoxin on the cell membrane potential. It is obvious that the increase in membrane conductance and depolarization of the cell membrane potential are due to an influx of Na+ into the cell accompanied by an efflux of K+ from the cell.
Cemerikic
D
D
Department of Pathological Physiology, Medical Faculty, Belgrade, Yugoslavia.
Nedeljkov
V
V
Lopicic
S
S
Dragovic
S
S
Beleslin
B
B
D000599:Amino Acids, Diamino / Q000633:toxicity*; D000818:Animals; D002118:Calcium / Q000378:metabolism; D004594:Electrophysiology; D027807:Lathyrus; D007865:Leeches; D008564:Membrane Potentials / Q000187:drug effects; D008839:Microelectrodes; D009474:Neurons / Q000187:drug effects* / Q000502:physiology*; D009498:Neurotoxins / Q000633:toxicity*; D011188:Potassium / Q000378:metabolism; D012964:Sodium / Q000378:metabolism
D000599:Amino Acids, Diamino; D009498:Neurotoxins; C100126:oxalyldiaminopropionic acid; D012964:Sodium; D011188:Potassium; D002118:Calcium