title: Neuroblastomas with chromosome 11q loss and single copy MYCN comprise a biologically distinct group of tumours with adverse prognosis.
abstract: Neuroblastoma is a heterogeneous tumour and its effective clinical management is dependent on accurate prognostic evaluation. In approximately 25% of patients amplification of the MYCN oncogene is known to be associated with a poor outcome. In order to identify additional molecular markers with prognostic potential in non-MYCN-amplified neuroblastomas, we looked for a correlation between clinical outcome and loss of heterozygosity (LOH) on four chromosomes that frequently show alteration in neuroblastoma (chromosomes 3, 4, 11 and 14). Chromosome 11q loss (with frequent parallel loss of chromosomes 3p, 4p and/or 14q) was found exclusively in tumours without MYCN amplification and was significantly associated with poor event-free survival. The 2-year event-free survival rate for 11q LOH cases was 30%, compared to 34% for MYCN-amplified cases and 100% for cases without these abnormalities. While 11q LOH was associated predominantly with advanced-stage disease, 2 cases with low-stage disease and 11q LOH both suffered relapses. We conclude that chromosome 11q loss defines a biologically distinct group of tumours without MYCN amplification that appear to have potential for aggressive metastatic growth. Thus this genetic alteration may be an important new prognostic marker in neuroblastoma.
authors.affiliation: Division of Medical and Molecular Genetics, University of Birmingham B15 2TT, UK.
authors.forename: M E
authors.initials: ME
authors.lastname: Luttikhuis
authors.forename: J E
authors.initials: JE
authors.lastname: Powell
authors.forename: S A
authors.initials: SA
authors.lastname: Rees
authors.forename: T
authors.initials: T
authors.lastname: Genus
authors.forename: S
authors.initials: S
authors.lastname: Chughtai
authors.forename: P
authors.initials: P
authors.lastname: Ramani
authors.forename: J R
authors.initials: JR
authors.lastname: Mann
authors.forename: C M
authors.initials: CM
authors.lastname: McConville
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis*; D002648:Child; D002675:Child, Preschool; D002880:Chromosomes, Human, Pair 11 / Q000235:genetics*; D018572:Disease-Free Survival; D005260:Female; D005784:Gene Amplification; D016259:Genes, myc / Q000235:genetics*; D006801:Humans; D007223:Infant; D008297:Male; D009362:Neoplasm Metastasis; D009447:Neuroblastoma / Q000235:genetics*; D011379:Prognosis
chemical_list: D014408:Biomarkers, Tumor