title: Natural history of experimental intestinal atresia: morphologic and ultrastructural study.
abstract: OBJECTIVE
  The aim of this study was to evaluate a natural history of congenital intestinal atresia (IA) in the chick embryo and to assess the type and nature of changes in the intestine at various developmental stages.
  
  
  METHODS
  Chick embryos underwent operative induction of IA on the 12th day of incubation. The procedure consisted of electrocoagulation of the mesenteric vessels supplying a 7- to 8-mm intestinal segment. The embryos were subjected to macroscopic examination, histologic and ultrastructural studies of the preatretic and postatretic bowel using the light microscope, scanning, and transmission electron microscopes. All investigations were performed in an experimental group (operated embryos), in a control group, and in a sham-operated group on the 15th, 17th, 19th, and 21st day of incubation.
  
  
  RESULTS
  The original technique of an iatrogenic "vascular event" proved to be effective because IA developed in 96% of embryos surviving the procedure. The affected portion of the bowel underwent progressive necrosis, and signs of bowel obstruction could be observed 48 hours after operation. Cord atresia (type II) developed in 81% of embryos. Histologic investigations showed progressive thinning of mucosa, flattening of mucosal folds, and epithelial detachment within the intestine proximal to atresia. There was only mild hypertrophy of the muscular layers. All these pathomorphologic changes were of rapidly progressive nature until the 17th day of incubation. Later, the rate of distension of preatretic bowel and histologic changes were less. Ultrastructural investigation of the proximal bowel showed progressive flattening of the enterocytes associated with their apical bulging, widening of the intercellular spaces, and microvilli atrophy. Surprisingly, at days 19 and 21 of incubation, signs of induction of adaptive mechanisms with partial restoration of near-normal microvilli pattern were observed.
  
  
  CONCLUSIONS
  Study of natural history of experimental IA indicates that histologic and ultrastructural lesions of the bowel are of dynamic nature and are not only the effect of pathologic intraluminal pressure.
authors.affiliation: Department of Paediatric Surgery, Wroclaw University of Medicine, Wroclaw, Poland.
authors.forename: S M
authors.initials: SM
authors.lastname: Baglaj
authors.forename: J
authors.initials: J
authors.lastname: Czernik
authors.forename: J
authors.initials: J
authors.lastname: Kuryszko
authors.forename: P
authors.initials: P
authors.lastname: Kuropka
mesh_terms: D000818:Animals; D002642:Chick Embryo; D046508:Culture Techniques; D004195:Disease Models, Animal; D007150:Immunohistochemistry; D007409:Intestinal Atresia / Q000473:pathology*; D007413:Intestinal Mucosa / Q000473:pathology* / Q000648:ultrastructure*; D008855:Microscopy, Electron, Scanning; D012016:Reference Values; D012680:Sensitivity and Specificity