title: Heterotypic docking of Cx43 and Cx45 connexons blocks fast voltage gating of Cx43.
abstract: Immunohistochemical co-localization of distinct connexins (Cxs) in junctional areas suggests the formation of heteromultimeric channels. To determine the docking effects of the heterotypic combination of Cx43 and Cx45 on the voltage-gating properties of their channels, we transfected DNA encoding Cx43 or Cx45 into N2A neuroblastoma or HeLa cells. Using a double whole-cell voltage-clamp technique, we determined macroscopic and single-channel gating properties of the intercellular channels formed. Cx43-Cx45 heterotypic channels had rectifying properties where Cx45 connexons inactivated rapidly upon hyperpolarizing voltage pulses applied to the Cx45-expressing cell. During depolarizing pulses to the Cx45-expressing cell, Cx43 connexons inactivated with substantially reduced kinetics as compared with homotypic Cx43 channels. Similar slow kinetics was observed for homotypic Cx43M257 (truncation mutant). Heterotypic channels had a main conductance whose value was predicted by the sum of corresponding homomeric connexon conductances; it was not voltage dependent and had no detectable residual conductance. The voltage-gating kinetics of heterotypic channels and their single-channel behavior implicate a role for the Cx43 carboxyl-terminal domain in the fast gating mechanism and in the establishment of residual conductance. Our results also suggest that heterotypic docking may lead to conformational changes that inhibit this action of the Cx43 carboxyl-terminal domain.
authors.affiliation: Krannert Institute of Cardiology, Indiana University, Indianapolis, Indiana 46202, USA.
authors.forename: S
authors.initials: S
authors.lastname: Elenes
authors.forename: A D
authors.initials: AD
authors.lastname: Martinez
authors.forename: M
authors.initials: M
authors.lastname: Delmar
authors.forename: E C
authors.initials: EC
authors.lastname: Beyer
authors.forename: A P
authors.initials: AP
authors.lastname: Moreno
mesh_terms: D000818:Animals; D015153:Blotting, Western; D018031:Connexin 43 / Q000737:chemistry / Q000378:metabolism*; D017630:Connexins / Q000737:chemistry / Q000378:metabolism*; D004553:Electric Conductivity; D017629:Gap Junctions / Q000737:chemistry / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D015640:Ion Channel Gating; D051379:Mice; D018408:Patch-Clamp Techniques; D014407:Tumor Cells, Cultured
chemical_list: D018031:Connexin 43; D017630:Connexins; C082920:connexin 45