Oxidative stress in polycystic ovary syndrome and its contribution to the risk of cardiovascular disease.
OBJECTIVE
To determine oxidant and antioxidant status in women with polycystic ovary syndrome (PCOS) and its contribution to the risk of cardiovascular disease.


METHODS
27 women with PCOS were compared with regard to oxidant and antioxidant status with 18 age- and body mass index (BMI)-matched healthy. Oxidant status was evaluated by determination of erythrocyte malondialdehyde (MDA) concentration, while antioxidant status was evaluated by determination of erythrocyte reduced glutathione (GSH) concentration, and glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) activities. Area under curve (AUC) for glucose, AUC for insulin and the insulin sensitivity index (ISI) were calculated from two-hour OGTT.


RESULTS
Women with PCOS were found to have higher AUC for glucose (p = 0.01), AUC for insulin (p < 0.001), MDA level (p = 0.009) and SOD activity (p = 0.04), and lower ISI (p < 0.001) and GSH level (p = 0.03) than the controls. In correlation analysis, a significant relationship was found between MDA levels and age (p < 0.01), BMI (p < 0.001), waist-to-hip ratio (p < 0.01), systolic and diastolic blood pressures (both p < 0.05), AUCs for glucose and insulin (both p < 0.05), ISI (r = -0.42, p < 0.05) and triglyceride (p < 0.01).


CONCLUSIONS
An increase in oxidant status was found in women with PCOS, and this increase was related to central obesity, age, blood pressure, serum glucose, insulin and triglyceride levels and insulin resistance. In contrast, antioxidant status was observed to be insufficient. These findings suggest that increased oxidative stress may contribute to the increased risk of cardiovascular disease in women with PCOS.
Sabuncu
T
T
Department of Endocrinology and Metabolism, University of Harran, Faculty of Medicine, Research Hospital, Sanliurfa, Turkey.
Vural
H
H
Harma
M
M
Harma
M
M
D000328:Adult; D001786:Blood Glucose / Q000032:analysis; D001794:Blood Pressure; D001824:Body Constitution; D015992:Body Mass Index; D002318:Cardiovascular Diseases / Q000150:complications*; D004912:Erythrocytes / Q000737:chemistry* / Q000201:enzymology; D005260:Female; D005979:Glutathione Peroxidase / Q000097:blood; D006801:Humans; D007328:Insulin / Q000097:blood; D007333:Insulin Resistance / Q000502:physiology; D015227:Lipid Peroxidation / Q000502:physiology; D008315:Malondialdehyde / Q000097:blood; D016555:Matched-Pair Analysis; D009765:Obesity; D018384:Oxidative Stress / Q000502:physiology*; D011085:Polycystic Ovary Syndrome / Q000150:complications* / Q000175:diagnosis / Q000000981:diagnostic imaging; D013482:Superoxide Dismutase / Q000097:blood; D014463:Ultrasonography
D001786:Blood Glucose; D007328:Insulin; D008315:Malondialdehyde; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase