Distribution of rotavirus-specific memory B cells in gut-associated lymphoid tissue after primary immunization.
We found previously that mice inoculated orally with simian rotavirus strain RRV developed virus-specific memory B cell responses 16 weeks after immunization that were greater than those found 6 weeks after immunization. Memory B cell responses were defined as the quantity of virus-specific IgA detected in small intestinal lamina propria (LP) fragment cultures of immunized mice at various intervals after challenge. Enhanced memory B cell responses correlated with enhanced protection against shedding. In order to understand better the delayed onset of rotavirus-specific memory B cell responses, a method was developed to determine the frequencies of rotavirus-specific memory B cells in gut-associated lymphoid tissues (GALT). We found that protection against rotavirus challenge was determined by the frequency of rotavirus-specific memory B cells in GALT LP.
Moser
Charlotte A
CA
Section of Infectious Diseases, The Children's Hospital of Philadelphia, Abramson Research Building, Room 1205A, 3516 Civic Center Blvd, Philadelphia, PA 19104, USA1.
Offit
Paul A
PA
The University of Pennsylvania School of Medicine2 and The Wistar Institute of Anatomy and Biology3, Philadelphia, PA 19104, USA.|Section of Infectious Diseases, The Children's Hospital of Philadelphia, Abramson Research Building, Room 1205A, 3516 Civic Center Blvd, Philadelphia, PA 19104, USA1.
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D001402:B-Lymphocytes / Q000276:immunology*; D005260:Female; D007114:Immunization; D007156:Immunologic Memory; D007413:Intestinal Mucosa / Q000276:immunology*; D008221:Lymphoid Tissue / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D012401:Rotavirus / Q000276:immunology*
D000914:Antibodies, Viral