A new species of Fusarium producer of galactose oxidase.
Fifty-two isolates of Fusarium species and one of Gibberella fujikuroi were tested for galactose oxidase (GO) production. Five Fusarium isolates contained GO activity in the culture filtrate: three F. graminearum and one each F. moniliforme f. sp. subglutinans and F. acuminatum. This is the first time F. acuminatum is reported to be a producer of GO enzyme. GO enzyme activity produced by isolates was assayed through a time course. Moreover, GO protein was partially purified from the most productive four isolates to show that the activity measured in the culture filtrates was due to the presence of GO protein.
Barbosa-Tessmann
I P
IP
Department of Biochemistry, State University of Maringá, Avenida Colombo, 5790, 87020-900, Maringá, PR, Brazil. ipbtessmann@uem.br
da Silva
D A
DA
Peralta
R M
RM
Kemmelmeier
C
C
D003470:Culture Media; D005670:Fusarium / Q000145:classification* / Q000201:enzymology*; D005692:Galactose Oxidase / Q000096:biosynthesis* / Q000302:isolation & purification; D013997:Time Factors
D003470:Culture Media; D005692:Galactose Oxidase