Ultrastructure and functional versatility of hirudinean botryoidal tissue.
In leeches, the botryoidal tissue is composed of two different cell types--granular botryoidal cells and flattened endothelial-like cells--localized in the loose connective tissue between the gut and the body wall sac. We have observed that the botryoidal tissue undergoes functional and structural modifications in response to the different needs arising during the life-cycle of the animal. In healthy, untreated leeches, botryoidal cells are organized in cords or clusters, sometimes surrounding few, small lacunae. Conversely, in wounded animals we have observed the transition of the botryoidal tissue from cluster/cord-like structures to a hollow/tubular architecture, typical of pre-vascular structures. We have documented in botryoidal cell cytoplasm the presence of large calcium storage. Moreover, the cytoplasm of botryoidal cells was filled with granules of different form and size, containing iron or melanin, as tested by classic histochemical methods. The presence of elements like iron and calcium was confirmed by the well-established EDS analysis. In response to a surgical wound, botryoidal tissue cells changed their shape and formed new capillary vessels. Concurrently, botryoidal cells secreted iron from cytoplasmic granules into the new cavity: this secretory activity appeared to be related to intracellular calcium fluctuations. At the end of the angiogenic process, botryoidal cells lost their contact with the basal lamina and moved freely in the circulating fluid towards the lesioned area. Interestingly, circulating botryoidal cells were found to carry melanin in the wounded area. This function is probably involved in defense processes. Thus, we have shown that stimulated botryoidal tissue displays a variety of striking structural, secretory and defensive activities.
de Eguileor
M
M
Department of Structural and Functional Biology, University of Insubria, Varese, Italy. magda.deeguileor@uninsubria.it
Grimaldi
A
A
Tettamanti
G
G
Congiu
T
T
Protasoni
M
M
Reguzzoni
M
M
Valvassori
R
R
Lanzavecchia
G
G
D000818:Animals; D002118:Calcium / Q000032:analysis; D000252:Calcium-Transporting ATPases / Q000032:analysis; D003239:Connective Tissue Cells / Q000502:physiology* / Q000648:ultrastructure; D003594:Cytoplasmic Granules / Q000648:ultrastructure; D004577:Electron Probe Microanalysis; D004727:Endothelium / Q000166:cytology / Q000648:ultrastructure; D006651:Histocytochemistry / Q000379:methods; D007501:Iron / Q000032:analysis; D007865:Leeches / Q000033:anatomy & histology*; D008543:Melanins / Q000032:analysis; D008855:Microscopy, Electron, Scanning; D018919:Neovascularization, Physiologic / Q000502:physiology; D014945:Wound Healing / Q000502:physiology; D014947:Wounds and Injuries / Q000503:physiopathology
D008543:Melanins; D007501:Iron; D000252:Calcium-Transporting ATPases; D002118:Calcium