The clinic study on synchronous detection of HCV RNA in the plasma and PBMC of hepatitis C patients.
In order to increase the positive detection rate of HCV RNA in the patients with chronic hepatitis C (CHC), RT-PCR was used to synchronously detect HCV RNA in the plasma and peripheral blood mononuclear cells (PBMC) of 583 CHC patients with a continuously elevated level of ALT for more than one year. The results showed that the positive detection rate of HCV RNA in the plasma of the CHC patients was 19.2%, while 24.5% in PBMC. It was demonstrated that the positive detection rate for HCV RNA in PBMC was obviously higher than that detected in plasma. To synchronously detect HCV RNA in PBMC by using RT-PCR can increase the positive detection rate of HCV RNA in the CHC patients.
Ye
J
J
Department of Infectious Disease, Xiehe Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430022.
Liu
W
W
Guo
J
J
Zeng
L
L
D000328:Adult; D005260:Female; D006526:Hepatitis C / Q000175:diagnosis* / Q000821:virology; D006518:Hepatitis Viruses / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000821:virology*; D012367:RNA, Viral / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D012367:RNA, Viral