Pectin secretion and distribution in the anther during pollen development in Lilium.
Using the monoclonal antibodies JIM 5 and 7, pectin was immunolocalized and quantitatively assayed in three anther compartments of Lilium hybrida during pollen development. Pectin levels in both the anther wall and the loculus increased following meiosis, were maximal during the early microspore stages and declined during the remainder of pollen ontogenesis. In the microspores/pollen grains, pectin was detectable at low levels during the microspore stages but accumulated significantly during pollen maturation. During early microspore vacuolation, esterified pectin epitopes were detected both in the tapetum cytoplasm and vacuoles. In the anther loculus, the same epitopes were located simultaneously in undulations of the plasma membrane and in the locular fluid. At the end of microspore vacuolation, esterified pectin epitopes were present within the lipids of the pollenkitt, and released in the loculus at pollen mitosis. Unesterified pectin epitopes were hardly detectable in the cytoplasm of the young microspore but were as abundant in the primexine matrix as in the loculus. During pollen maturation, both unesterified and esterified pectin labelling accumulated in the cytoplasm of the vegetative cell, concurrently with starch degradation. In the mature pollen grain, unesterified pectin epitopes were located in the proximal intine whereas esterified pectin epitopes were deposited in the distal intine. These data suggest that during early microspore development, the tapetum secretes pectin, which is transferred to the primexine matrix via the locular fluid. Further, pectin is demonstrated to constitute a significant component of the pollen carbohydrate reserves in the mature grain of Lilium.
Aouali
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N
Université de Reims Champagne Ardenne, UFR Sciences, Biologie et Physiologie Végétales, France.
Laporte
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P
Clément
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C
D000911:Antibodies, Monoclonal; D002454:Cell Differentiation; D002455:Cell Division; D002462:Cell Membrane / Q000648:ultrastructure; D002473:Cell Wall / Q000737:chemistry / Q000648:ultrastructure; D003593:Cytoplasm / Q000648:ultrastructure; D000939:Epitopes; D007150:Immunohistochemistry; D019668:Liliaceae / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism; D010368:Pectins / Q000378:metabolism*; D011058:Pollen / Q000254:growth & development* / Q000378:metabolism / Q000648:ultrastructure; D012098:Reproduction; D013213:Starch / Q000378:metabolism; D014617:Vacuoles / Q000648:ultrastructure
D000911:Antibodies, Monoclonal; D000939:Epitopes; D010368:Pectins; D013213:Starch