title: Prevention of ventilator-induced lung injury with partial liquid ventilation.
abstract: OBJECTIVE
  Pulmonary injury from mechanical ventilation has been attributed to application of excess alveolar pressure (barotrauma) or volume (volutrauma). The authors questioned whether partial liquid ventilation (gas ventilation of the perfluorocarbon filled lung, PLV) would reduce ventilator-induced lung injury.
  
  
  METHODS
  A tracheostomy tube and carotid artery catheter were placed in anesthetized Sprague-Dawley rats (500 +/- 50 g). Bovine serum albumin (BSA) labeled with Iodine (I) 125 was administered intraarterially. Ventilation with tidal volume (TV) of 5 mL/kg was initiated. The rats were then selected randomly to a 30-minute experimental period of one of the following ventilation protocols: continued atraumatic gas ventilation (GV, TV, 5 mL/kg; n = 10); atraumatic gas ventilation combined with intratracheal administration of 10 mL/kg perfluorocarbon (GV-PLV, TV, 5 mL/kg, n = 10); barotrauma (BT, peak inspiratory pressure [PIP], 45 cm H(2)O; n = 10); barotrauma with PLV (BT-PLV, PIP, 45 cm H(2)O; n = 8); volutrauma (VT, TV, 30 mL/kg; n = 8); or volutrauma with PLV (VT-PLV, TV, 30 mL/kg; n = 10). Animals were killed and the amount of radiolabeled BSA in both lungs was measured and normalized to the counts in 1 mL of blood from that animal (injury index). Data were analyzed by analysis of variance (ANOVA) with post-hoc t test comparison between groups.
  
  
  RESULTS
  There was a significant difference in the (125)I-BSA injury index when all groups were compared (P <.001 by ANOVA). Post-hoc analysis showed a significant decrease in the injury index when comparing BT versus BT-PLV (P =.024) and VT versus VT-PLV (P =.014).
  
  
  CONCLUSIONS
  (125)I-BSA leak produced during high-pressure or high-volume mechanical ventilation is reduced by partial liquid ventilation.
authors.affiliation: Department of Surgery and Pathology at the University of Michigan Medical Center, Ann Arbor, MI, USA.
authors.forename: D A
authors.initials: DA
authors.lastname: Lewis
authors.forename: D
authors.initials: D
authors.lastname: Colton
authors.forename: K
authors.initials: K
authors.lastname: Johnson
authors.forename: R B
authors.initials: RB
authors.lastname: Hirschl
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001469:Barotrauma / Q000150:complications*; D004195:Disease Models, Animal; D021061:Liquid Ventilation / Q000379:methods*; D008171:Lung Diseases / Q000209:etiology / Q000517:prevention & control*; D055370:Lung Injury; D008297:Male; D011336:Probability; D051381:Rats; D017207:Rats, Sprague-Dawley; D012121:Respiration, Artificial / Q000009:adverse effects* / Q000379:methods; D012680:Sensitivity and Specificity; D016896:Treatment Outcome