title: Cytochemical analysis of acid phosphatase activity in the venom secretory cells of Bothrops jararaca.
abstract: A study of the histochemical reaction for acid phosphatase (AcPase) in venom gland secretory cells from Bothrops jararaca was done to investigate the distribution of lysosomes and related structures in stages of high- and low-protein synthesis. From this analysis, it was expected to gain insight into the cellular pathway by which AcPase is secreted into the venom. Two subtypes of AcPase reactivities were detected in the venom gland secretory cells: one was found in lysosomes and related structures and in some trans-Golgi network (TGN) elements and reacts with beta-glycerophosphate (betaGP) as substrate; the other was found in secretory vesicles, apical plasmalemma, lysosomes and related structures, and in some TGN elements, and reacts with cytidine monophosphate (CMP). The results are compatible with the possibility that there is a secretory via for AcPase in the venom gland of B. jararaca and that the elements composing this pathway are noted only when CMP is used as substrate. Large autophagosomes reactive to both betaGP and to CMP were commonly observed in the basal region of the secretory cells, and they were more abundant in the glands during the stage of low activity of protein synthesis.
authors.affiliation: Instituto Butantan Laboratório de Biologia Celular, São Paulo-SP, Brasil. sycarneiro@hotmail.com
authors.forename: S M
authors.initials: SM
authors.lastname: Carneiro
authors.forename: W
authors.initials: W
authors.lastname: Fernandes
authors.forename: M S
authors.initials: MS
authors.lastname: Della Casa
authors.forename: A
authors.initials: A
authors.lastname: Sesso
mesh_terms: D000135:Acid Phosphatase / Q000032:analysis*; D000818:Animals; D017837:Bothrops / Q000033:anatomy & histology / Q000378:metabolism* / Q000502:physiology; D002462:Cell Membrane / Q000201:enzymology / Q000648:ultrastructure; D003568:Cytidine Monophosphate / Q000378:metabolism; D005994:Glycerophosphates / Q000378:metabolism; D006056:Golgi Apparatus / Q000201:enzymology / Q000648:ultrastructure; D006651:Histocytochemistry / Q000379:methods; D008247:Lysosomes / Q000201:enzymology / Q000648:ultrastructure; D022142:Secretory Vesicles / Q000201:enzymology / Q000648:ultrastructure; D013379:Substrate Specificity; D014018:Tissue Distribution; D014688:Venoms / Q000201:enzymology*
chemical_list: D005994:Glycerophosphates; D014688:Venoms; D000135:Acid Phosphatase; D003568:Cytidine Monophosphate; C031463:beta-glycerophosphoric acid