Poly(adp-ribose) synthetase inhibition reduces bacterial translocation in rats after endotoxin challenge.
We investigated whether 3-aminobenzamide (3-AB), a poly(ADP-ribose) synthetase (PARS) inhibitor, reduces bacterial translocation (BT) after intraperitoneal endotoxin administration. Wistar rats were randomized to receive intraperitoneal saline (control, n = 6); endotoxin (n = 8); 3-AB (n = 6); and 3-AB plus endotoxin (n = 8). Six hours later, to evaluate the endotoxin-related intestinal injury and BT, tissue and blood samples were collected. Administration of intraperitoneal endotoxin caused severe intestinal injury and BT to mesenteric lymph nodes. PARS inhibition with 3-AB completely prevented endotoxin-induced BT. No colony-forming bacteria was isolated from the samples obtained from 3-AB-pretreated animals under endotoxin challenge. Treatment with 3-AB significantly reduced the endotoxin-induced intestinal mucosal injury. The inhibition of PARS by its blocker 3-aminobenzamide during endotoxemia prevents bacterial translocation and intestinal injury in rats. PARS activation may provide a novel therapeutic approach in reducing gut barrier failure seen in endotoxemia.
Taner
A S
AS
Department of Surgery, Ankara State Hospital, Turkey.
Cinel
I
I
Ozer
L
L
Onde
U
U
Taner
D
D
Koksoy
C
C
D000818:Animals; D018988:Bacterial Translocation / Q000187:drug effects* / Q000502:physiology; D001549:Benzamides; D003114:Colony-Forming Units Assay; D004195:Disease Models, Animal; D019446:Endotoxemia / Q000188:drug therapy / Q000382:microbiology*; D004731:Endotoxins / Q000633:toxicity*; D004791:Enzyme Inhibitors / Q000627:therapeutic use*; D007421:Intestine, Small / Q000473:pathology; D008297:Male; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D051381:Rats; D017208:Rats, Wistar
D001549:Benzamides; D004731:Endotoxins; D004791:Enzyme Inhibitors; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; C025160:3-aminobenzamide