Effects of Puerariae Flos and Puerariae Radix extracts on antioxidant enzymes in ethanol-treated rats.
This study was performed to investigate the effect of Puerariae Flos (PF) and Puerariae Radix (PR) water extracts on the activities and mRNA expression of three hepatic antioxidant enzymes in ethanol-treated rats. Male Sprague-Dawley rats were divided into four groups, a control, ethanol-treated, ethanol plus PF-treated, and ethanol plus PR-treated group with seven rats per group. Ethanol (25 % v/v, 5 g/kg body weight) was orally administered once a day for 5 weeks. The PF and PR water extracts were supplemented in a diet based on 1.2 g of raw PF or PR/kg body weight/day. Ethanol administration without the PF or PR supplement significantly lowered the activities of hepatic Cu/Zn SOD and catalase (CAT), whereas it increased the hepatic GSH-Px activity. However, the PF and PR supplementation resulted in a significant increase in the Cu/Zn SOD and/or CAT activities and a significant decrease in the GSH-Px activity in the ethanol-treated rats. The mRNA levels of these antioxidant enzymes in the ethanol-treated rats were normalized to the control level by the PF or PR supplement. The hepatic glutathione content, which was significantly lower in the ethanol-treated group than in the control group, was also normalized to the control level by supplementing with either PF or PR. The PF or PR supplement resulted in lowering the hepatic malondialdehyde to the control level in the ethanol-treated rats.
Lee
M K
MK
Department of Genetic Engineering, Kyungpook National University, Taegu, Korea.
Cho
S Y
SY
Jang
J Y
JY
Cho
M S
MS
Jeon
S M
SM
Jang
M K
MK
Kim
M J
MJ
Park
Y B
YB
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D015152:Blotting, Northern; D002374:Catalase / Q000378:metabolism; D002492:Central Nervous System Depressants / Q000378:metabolism*; D003300:Copper / Q000378:metabolism; D015342:DNA Probes; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D000431:Ethanol / Q000378:metabolism*; D005979:Glutathione Peroxidase / Q000378:metabolism; D066298:In Vitro Techniques; D008099:Liver / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D008516:Medicine, Chinese Traditional; D018384:Oxidative Stress / Q000187:drug effects*; D029908:Pueraria; D012333:RNA, Messenger / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013482:Superoxide Dismutase / Q000378:metabolism; D015032:Zinc / Q000378:metabolism
D000975:Antioxidants; D002492:Central Nervous System Depressants; D015342:DNA Probes; D004365:Drugs, Chinese Herbal; C442841:Puerariae Flos extract; D012333:RNA, Messenger; C442842:plant extract, Puerariae Radix; D000431:Ethanol; D003300:Copper; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D015032:Zinc