[Effect of p-chloromercuribenzoic acid and dithiothreitol on the Ca(2+) content of salivary glands and their protein secretion].
It has been shown, less concentrations of p-chlormercuribenzoate (1 and 2.5 mM) increased Ca2+ content in gland tissue and thereby protein secretion level that may occurred mainly by suppression Ca(2+)-pump or/and stimulation of Na(+)-Ca(2+)-exchange (both in cell plasma membrane) through modulation of SH-groups which form part of their molecules. Higher PCMB concentrations markedly decreased Ca2+ content in gland tissue as well as protein secretion. Effects of PCMB (5 and 10 mM), depending on the direction of Na(+)-Ca(2+)-exchange functioning (Ca(2+)-efflux or Ca(2+)-influx), were evoked or presumably by suppression of endoplasma reticulum Ca(2+)-pump (at conditions Na(+)-dependent Ca(2+)-efflux) or Na(+)-dependent Ca(2+)-influx into the cells that clearly confirmed when PCMB was added on the background of eosin Y (specific Ca(2+)-ATPase inhibitor). Possible role of potential dependent Ca(2+)-channnels in the mediating of PCMB effects is discussed. Introducing of dythiothreitol (DTT) increased Ca2+ content in glands and decreased secretion level obviously by protection of SH-groups of cell Ca(2+)-transporting systems and thereby diminished [Ca2+]i. Finally, we confirm important functional role of SH-groups in the regulation of Ca(2+)-homeostasis in secretory cells of exocrine glands.
Fedirko
N V
NV
Ivan Franko Nataional University of Lviv.
Man'ko
V V
VV
Klevets'
M Iu
MIu
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D002683:Chironomidae; D004229:Dithiothreitol / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004801:Eosine Yellowish-(YS) / Q000494:pharmacology; D006706:Homeostasis; D066298:In Vitro Techniques; D019476:Insect Proteins / Q000187:drug effects / Q000378:metabolism*; D012469:Salivary Glands / Q000187:drug effects* / Q000378:metabolism; D019831:Sodium-Calcium Exchanger / Q000187:drug effects / Q000378:metabolism; D013438:Sulfhydryl Compounds / Q000378:metabolism; D013439:Sulfhydryl Reagents / Q000494:pharmacology*; D020245:p-Chloromercuribenzoic Acid / Q000494:pharmacology*
D019476:Insect Proteins; D019831:Sodium-Calcium Exchanger; D013438:Sulfhydryl Compounds; D013439:Sulfhydryl Reagents; D020245:p-Chloromercuribenzoic Acid; D000252:Calcium-Transporting ATPases; D002118:Calcium; D004229:Dithiothreitol; D004801:Eosine Yellowish-(YS)
Vplyv parakhlormerkuriĭbenzoatu ta dytiotreitolu na vmist Ca(2+) u tkanyni slynnykh zaloz ta sekretsiiu nymy zahal'noho bilka.