Isolation and characterization of psychotrophic bacteria from oil-reservoir water and oil sands.
Four psychrotrophic strains, which grew at 4 degrees C but not at 37 degrees C, were isolated from Japanese oil-reservoir water (strains SIB1, SIC1, SIS1) and Canadian oil sands (strain CAB1). Strains SIB1, SIS1, and CAB1 had a maximum growth rate at 20 degrees C and grew to the highest cell densities at the cultivation temperature of 0-4 degrees C. Strain SIS1 was capable of growing even at -5 degrees C. The growth profile of strain SIC1 was rather similar to that of a mesophilic bacterium. Strains SIB1, SIC1, and SIS1 were identified as members of the genus Shewanella, and strain CAB1 was a member of the genus Arthrobacter. All these strains exhibited weak degradation ability against catechol, a hydroxylated aromatic hydrocarbon, and tributyrin. These strains are expected to be of potential use in the in situ bioremediation technology of hazardous hydrocarbons and esters under low-temperature conditions.
Kato
T
T
Department of Material and Life Science, Graduate School of Engineering, Osaka University, Suita, Japan.
Haruki
M
M
Imanaka
T
T
Morikawa
M
M
Kanaya
S
S
D000332:Aerobiosis; D000473:Alkanes / Q000378:metabolism; D001173:Arthrobacter / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism; D001673:Biodegradation, Environmental; D002170:Canada; D002396:Catechols / Q000378:metabolism; D003080:Cold Temperature; D020459:Genes, rRNA; D007564:Japan; D009281:Naphthalenes / Q000378:metabolism; D010578:Petroleum; D020592:Shewanella / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism; D012822:Silicon Dioxide; D012988:Soil Microbiology; D012989:Soil Pollutants; D014280:Triglycerides / Q000378:metabolism; D014871:Water Microbiology; D014876:Water Pollution
D000473:Alkanes; D002396:Catechols; D009281:Naphthalenes; D010578:Petroleum; D012989:Soil Pollutants; D014280:Triglycerides; C031721:naphthalene; D012822:Silicon Dioxide; C034221:catechol; C005830:tributyrin