[Assay in high throughput screening].
HTS (High Throughput Screening) has been put into practice in recent years. HTS is aiming to discover lead compounds for medicinal drugs. High efficiency must be achieved in all the processes including sample preparation, assay procedure, automation and data management. This review will focus on the aspects concerned with the assay technology and the efficiency in HTS. One of the major trends in HTS is assay miniaturization using high-density microplates with 384 and 1536 wells. This allows us to increase the throughput and to decrease the cost. The so-called "mix and measure" or "homogeneous" assay system, which has no separation steps such as washing or filtration, is effective for this purpose. The homogeneous assays, such as scintillation proximity assay (SPA), fluorescence energy transfer (HTRF, LANCE) and fluorescence polarization (FP), are frequently used. The reporter gene assay or the cell proliferation assay can be adapted for the homogeneous assay using high-density plates. In addition, HTS measuring the intracellular Ca2+ influx is also possible using a CCD Imager. The assay quality as well as the efficiency is also important especially in HTS. The Z'-factor provides a good tool for evaluating the quality of assays.
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Business Strategy Division, Taisho Pharmaceutical Co. Ltd., Saitama 330-8530, Japan. s16751@ccm.taisho.co.jp
D001681:Biological Assay / Q000295:instrumentation / Q000379:methods; D002623:Chemistry Techniques, Analytical / Q000295:instrumentation / Q000379:methods; D002626:Chemistry, Pharmaceutical / Q000295:instrumentation / Q000379:methods*; D015195:Drug Design; D005454:Fluorescence Polarization; D008904:Miniaturization; D011868:Radioisotopes; D012588:Scintillation Counting; D013050:Spectrometry, Fluorescence
D011868:Radioisotopes