[Simultaneous expression of chicken anaemia virus proteins VP1 and VP2 in silkworms].
By cloning vp1 and vp2 genes of chicken anaemia virus into transfer vector pBacPAK8, recombinant transfer plasmids pBac-vp1 and pBac-vp2 were obtained. Then BmN cells were co-transfected with linearized baculovirus Bm-BacPAK6 DNA and above two recombinant plasmids respectively, recombinant viruses Bm-vp1 and Bm-vp2 were constructed and used to co-infect silkworms to express recombinant proteins. The results indicated that recombinant VP1 and VP2 could induce the corresponding antibody in chickens using immunofluorescence assay and the expression products could protect filial generation from the attack of CAV. Recombinant BmNPV expressing VP1 and VP2 is, therefore, a great hopeful production system for a subunit vaccine against CAV infection.
Xiao
Q L
QL
Key Laboratory of Silkworm Biotechnology, Ministry of Agriculture, Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, China.
Zhang
Z F
ZF
He
J L
JL
D000818:Animals; D012831:Bombyx / Q000235:genetics*; D002213:Capsid / Q000096:biosynthesis* / Q000276:immunology; D036022:Capsid Proteins; D002645:Chickens; D011994:Recombinant Proteins / Q000096:biosynthesis*; D014614:Vaccines, Synthetic / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology
D036022:Capsid Proteins; D011994:Recombinant Proteins; C442548:VP1 protein, Chicken anemia virus; D014614:Vaccines, Synthetic; D014765:Viral Vaccines