title: Studies on the mechanism of action of a targeted chemotherapeutic drug in living cancer cells by two photon laser scanning microspectrofluorometry.
abstract: In this study, we present a spectroscopic study of the entry pattern of a chemotherapeutic drug (AN-152) and its carrier hormone ([D-Lys(6)]LH-RH) into living cancer cells, with the help of our two-photon probes and a home-built localized microspectrofluorometer coupled with two photon laser scanning microscope (TPLSM). Due to the inherent localization ability of TPLSM, we were able to identify the drug and carrier location in different compartments of the cancer cells in vitro. The apparent doxorubicin-assisted nucleic accumulation of AN-152 suggests a possible nuclear action of the drug on cell proliferation.
authors.affiliation: Institute for Lasers, Photonics and Bio-Photonics, Department of Chemistry, SUNY at Buffalo, Buffalo, New York 14260, USA.
authors.forename: X
authors.initials: X
authors.lastname: Wang
authors.forename: H E
authors.initials: HE
authors.lastname: Pudavar
authors.forename: R
authors.initials: R
authors.lastname: Kapoor
authors.forename: L J
authors.initials: LJ
authors.lastname: Krebs
authors.forename: E J
authors.initials: EJ
authors.lastname: Bergey
authors.forename: C
authors.initials: C
authors.lastname: Liebow
authors.forename: P N
authors.initials: PN
authors.lastname: Prasad
authors.forename: A
authors.initials: A
authors.lastname: Nagy
authors.forename: A V
authors.initials: AV
authors.lastname: Schally
mesh_terms: D000970:Antineoplastic Agents / Q000493:pharmacokinetics / Q000627:therapeutic use*; D004317:Doxorubicin / Q000031:analogs & derivatives / Q000493:pharmacokinetics / Q000494:pharmacology*; D004337:Drug Carriers; D016503:Drug Delivery Systems; D007987:Gonadotropin-Releasing Hormone / Q000031:analogs & derivatives / Q000493:pharmacokinetics / Q000494:pharmacology*; D006801:Humans; D007834:Lasers; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D017785:Photons; D013050:Spectrometry, Fluorescence; D014018:Tissue Distribution; D014407:Tumor Cells, Cultured / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D000970:Antineoplastic Agents; D004337:Drug Carriers; C080883:LHRH, lysine(6)-doxorubicin; D007987:Gonadotropin-Releasing Hormone; C035029:LHRH, Lys(6)-; D004317:Doxorubicin