title: Different levels of Schistosoma mansoni infection increased the mutagenicity of benzo(a)pyrene, the activity of aryl hydrocarbon hydroxylase and the formation of hepatic microsomal hydrogen peroxide.
abstract: The present study investigates the influence of different levels of Schistosoma mansoni infection (60, 120, 180, 300, 600 cercariae per mice) after 33 days on the activity of aryl hydrocarbon hydroxylase (AHH) and the formation of hepatic hydrogen peroxide (H(2)O(2)) during the metabolic activation of benzo(a)pyrene [B(a)p]. Also, it shows the mutagenic effect of B(a)p at different levels of S. mansoni infection using Salmonella typhimurium TA 98 and TA 102 as a tester strains. High levels of H(2)O(2) production (222 nmol/mg protein) and AHH activity (240 pmol 3-OH B(a)p per mg protein) were seen at 300 cercariae per mice. Increasing histidine revertant colonies at TA98 and TA102 were detected at different levels of S. mansoni infection. These data clearly demonstrate that S. mansoni infection changes the mutagenicity of B(a)p, AHH activity, as well as enhancing the formation of hepatic H(2)O(2) generated during the metabolic activation of B(a)p in infected mice.
authors.affiliation: Department of Environmental Studies, Institute of Graduate Studies and Research, Alexandria University, Alexandria, Egypt. hawney134@yahoo.com
authors.forename: H A
authors.initials: HA
authors.lastname: Awney
authors.forename: H E
authors.initials: HE
authors.lastname: Ghzlan
authors.forename: S A
authors.initials: SA
authors.lastname: Sheweita
authors.forename: M H
authors.initials: MH
authors.lastname: Mostafa
mesh_terms: D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases / Q000378:metabolism*; D001564:Benzo(a)pyrene / Q000633:toxicity*; D001711:Biotransformation; D004781:Environmental Exposure; D006861:Hydrogen Peroxide / Q000032:analysis / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008862:Microsomes, Liver / Q000201:enzymology / Q000378:metabolism*; D009152:Mutagenicity Tests; D011506:Proteins / Q000032:analysis; D012555:Schistosomiasis mansoni / Q000235:genetics / Q000503:physiopathology*
chemical_list: D011506:Proteins; D001564:Benzo(a)pyrene; D006861:Hydrogen Peroxide; D001189:Aryl Hydrocarbon Hydroxylases