Single-copy primary transformants of maize obtained through the co-introduction of a recombinase-expressing construct.
We describe a variation of the method to generate single-copy transgenic plants by recombinase-mediated resolution of multiple insertions. In this study, a transgene construct flanked by oppositely oriented lox sites was co-bombarded into maize cells along with a cre-expressing construct. From analysis of the regenerated plants, a high percentage of the primary transformants harbored a single copy of the introduced transgene, and among these, a majority also lacked the cre construct. We deduce that the expression of cre must have contributed to resolving concatemeric molecules either prior to or after DNA integration into the maize genome.
Srivastava
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Plant Gene Expression Center, USDA-ARS, Albany, CA 94710, USA.
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D018628:Gene Dosage; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics; D019426:Integrases / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics; D014162:Transfection / Q000379:methods; D014170:Transformation, Genetic; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism; D003313:Zea mays / Q000235:genetics*
D014764:Viral Proteins; C045073:Cre recombinase; D019426:Integrases