title: [Alterations of the basal membrane in middle ear cholesteatoma].
abstract: Cholesteatoma epithelium is characterized by a keratinocyte disregulation accompanied by destruction of the ossicles and other bony parts of the temporal bone. Immunohistochemical methods using antibodies to fibronectin, tenascin and metalloproteinases were used to assess the alterations of the instrinsic and extrinsic components of the basement membrane. Spatial orientation of the basement membrane was preserved in histological sections. Collagen type IV, tenascin, fibronectin, basic fibroblastic growth factor (bFGF), and matrix metalloproteinases (MMPs) are related to the matrix, perimatrix of normal or pathological tissues. They were studied immunohistologically in twenty cholesteatomas, eight samples of normal auditory canal skin, and six specimens of normal middle ear mucosa. Cholesteatomas displayed alterations of the basal membrane, with presence of MMPs and a linear immunoreactivity for collagen type IV and laminin, disrupted in areas with intense inflammation. The electronic microscope revealed protrusions, duplications, thickening and disruptions of the lamina densa of the basement membrane. Thus, we conclude, that MMPs and bFGF could play an important role maintaining the proliferative activity and the aggressive behaviour of cholesteatoma in the middle ear.
authors.affiliation: Servicio de ORL, Hospital Clínic, Barcelona. mbernal@clinic.ub.es
authors.forename: M
authors.initials: M
authors.lastname: Bernal Sprekelsen
authors.forename: J
authors.initials: J
authors.lastname: Ebmeyer
authors.forename: A
authors.initials: A
authors.lastname: Anonopoulos
authors.forename: G
authors.initials: G
authors.lastname: Borkowski
authors.forename: H
authors.initials: H
authors.lastname: Sudhoff
mesh_terms: D001489:Basilar Membrane / Q000473:pathology* / Q000648:ultrastructure; D018424:Cholesteatoma, Middle Ear / Q000378:metabolism / Q000473:pathology*; D003094:Collagen / Q000378:metabolism; D046508:Culture Techniques; D004432:Ear, Middle / Q000473:pathology*; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism; D005353:Fibronectins / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D019063:Tenascin / Q000378:metabolism; D019714:Tissue Inhibitor of Metalloproteinases / Q000378:metabolism
chemical_list: D005353:Fibronectins; D019063:Tenascin; D019714:Tissue Inhibitor of Metalloproteinases; D016222:Fibroblast Growth Factor 2; D003094:Collagen
vernacular_title: Alteraciones de la membrana basal en el colesteatoma de oído medio.