Internodal specializations of myelinated axons in the central nervous system.
We have examined the localization of contactin-associated protein (Caspr), the Shaker-type potassium channels, Kv1.1 and Kv1.2, their associated beta subunit, Kvbeta2, and Caspr2 in the myelinated fibers of the CNS. Caspr is localized to the paranodal axonal membrane, and Kv1.1, Kv1.2, Kvbeta2 and Caspr2 to the juxtaparanodal membrane. In addition to the paranodal staining, an internodal strand of Caspr staining apposes the inner mesaxon of the myelin sheath. Unlike myelinated axons in the peripheral nervous system, there was no internodal strand of Kv1.1, Kv1.2, Kvbeta2, or Caspr2. Thus, the organization of the nodal, paranodal, and juxtaparanodal axonal membrane is similar in the central and peripheral nervous systems, but the lack of Kv1.1/Kv1.2/Kvbeta2/Caspr2 internodal strands indicates that the oligodendrocyte myelin sheaths lack a trans molecular interaction with axons, an interaction that is present in Schwann cell myelin sheaths.
Arroyo
E J
EJ
Department of Neurology, The University of Pennsylvania, Philadelphia 19104, USA.
Xu
T
T
Poliak
S
S
Watson
M
M
Peles
E
E
Scherer
S S
SS
D000818:Animals; D051662:Kv1.1 Potassium Channel; D051663:Kv1.2 Potassium Channel; D008565:Membrane Proteins; D051379:Mice; D009413:Nerve Fibers, Myelinated / Q000737:chemistry / Q000648:ultrastructure*; D009419:Nerve Tissue Proteins / Q000032:analysis*; D009836:Oligodendroglia / Q000737:chemistry / Q000648:ultrastructure; D015221:Potassium Channels / Q000032:analysis*; D024642:Potassium Channels, Voltage-Gated; D011901:Ranvier's Nodes / Q000737:chemistry*; D051381:Rats; D012583:Schwann Cells / Q000737:chemistry / Q000648:ultrastructure; D013116:Spinal Cord / Q000166:cytology*
C403049:CNTNAP2 protein, human; C496947:Kcna1 protein, mouse; C496970:Kcna2 protein, mouse; C496972:Kcna2 protein, rat; C426239:Kcnab2 protein, rat; D051663:Kv1.2 Potassium Channel; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D015221:Potassium Channels; D024642:Potassium Channels, Voltage-Gated; D051662:Kv1.1 Potassium Channel