[Specific regulated endonuclease activity of small RNP, containing prosomes from the A431 cell line: possible mechanisms of regulating the stability of RNA by epidermal growth factor].
A comparative study was made of reactive oxygen species (ROS) in rat embryo fibroblasts and their transformants. Primary rat embryo fibroblasts (REF), REF transformed by the complementing oncogenes E1A plus cHa-ras (cell line E1A + Ras), and REF transformed by E1A plus E1B-19 kDa (cell line E1A + E1B) were studied. ROS generation was measured with microfluorometric assay using fluorescent probe 2',7'-dichlorofluorescin diacetate. It has been shown that the block of REF and E1A + 1B cells in the G1/S under serum-starved conditions (0.5% serum) for 24-48 h was paralleled by a decrease in ROS generation. Activation of serum-starved REF and E1A + 1B cells with 10% serum resulted in reactivation of cell cycle and gradual increase in ROS generation. The maximum intracellular level of ROS correlated in time with the phase of DNA synthesis. Serum-starved E1A + Ras cells were not stopped in the G1/S and ROS production of these cells was not dependent on serum growth factors. The prolonged cultivation of E1A + Ras cells in the medium with low serum content (0.5%) caused a sharp increase in ROS generation, which was accompanied by apoptotic death.
Kulichkova
V A
VA
Institute of Cytology RAS, St. Petersburg.
Evteeva
I N
IN
Mittenberg
A G
AG
Obukhova
A D
AD
Volkova
I V
IV
Ermolaeva
Iu B
IuB
Teslenko
L V
LV
Medvedeva
N D
ND
Gauze
L N
LN
Konstantinova
I M
IM
D020413:3' Untranslated Regions; D004722:Endoribonucleases / Q000378:metabolism*; D004815:Epidermal Growth Factor / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008970:Molecular Weight; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017411:Ribonucleoproteins, Small Nuclear / Q000378:metabolism*; D015398:Signal Transduction; D014407:Tumor Cells, Cultured
D020413:3' Untranslated Regions; D012333:RNA, Messenger; D017411:Ribonucleoproteins, Small Nuclear; D004815:Epidermal Growth Factor; D004722:Endoribonucleases
Spetsificheskaia reguliruemaia éndoribonukleaznaia aktivnost' malykh RNP, soderzhashchikh prosomy, iz kletok linii A431: vozmozhnyĭ mekhanizm reguliatsii stabil'nosti RNK épidermal'nym faktorom rosta.