Region-specific decrease in 5-HT1A and 5-HT1B binding sites after intra-hippocampal ibotenic acid injections in the rat.
The cellular location of 5-HT1A and 5-HT1B binding sites in the hippocampus was investigated using a stereotaxic unilateral lesion of the CA1 field by ibotenic acid, followed by autoradiography on brain sections with the specific ligands [3H]8-OH-DPAT and S-CM-G[125I]TNH2, respectively. As compared to the contralateral side of the lesion, the ipsilateral side exhibited a decrease in the density of 5-HT1A binding sites in the strata oriens and radiatum of CA1, and of 5-HT1B binding sites in the dorsal subiculum and stratum oriens of CA1. The results demonstrate that 5-HT1A binding sites are located on dendrites whereas 5-HT1B binding sites are located on axon terminals of CA1 pyramidal cells.
Aït Amara
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INSERM U.488 Stéroïdes et Système Nerveux, 80 rue du Général Leclerc, 94276, Bicêtre, France.
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D017371:8-Hydroxy-2-(di-n-propylamino)tetralin / Q000378:metabolism; D000818:Animals; D001345:Autoradiography; D001665:Binding Sites / Q000187:drug effects; D018690:Excitatory Amino Acid Agonists / Q000008:administration & dosage / Q000494:pharmacology*; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D007051:Ibotenic Acid / Q000008:administration & dosage / Q000494:pharmacology*; D007267:Injections; D007274:Injections, Intraperitoneal; D008297:Male; D009474:Neurons / Q000187:drug effects; D051381:Rats; D020318:Rats, Long-Evans; D044302:Receptor, Serotonin, 5-HT1B; D011985:Receptors, Serotonin / Q000378:metabolism*; D044263:Receptors, Serotonin, 5-HT1
D018690:Excitatory Amino Acid Agonists; D044302:Receptor, Serotonin, 5-HT1B; D011985:Receptors, Serotonin; D044263:Receptors, Serotonin, 5-HT1; D007051:Ibotenic Acid; D017371:8-Hydroxy-2-(di-n-propylamino)tetralin