Expression of arginine vasopressin and vasopressin V1a receptor mRNA in diabetic (db/db) mice.
To assess the involvement of arginine vasopressin (AVP) in genetical diabetic (db/db) mice, we examined the mRNA expression levels of AVP and vasopressin V(1a) receptors (V(1a)R) in brain and liver of db/db mice. In 10 week-old db/db mice, a significant elevation in blood sugar levels and plasma osmolality were observed, showing obvious diabetic symptoms. There was a significant increase in brain AVP mRNA levels in db/db mice. The expression level of liver V(1a)R mRNA in db/db mice was significantly down-regulated, presumably as a consequence of ligand-receptor interaction. This is in contrast to results that show no significant reduction in brain V(1a)R mRNA levels when comparing db/db and control mice. Thus, it is possible that in the progress of genetic diabetes mellitus, AVP acts in liver than in brain through V(1a)R.
Morita
M
M
Advanced Technological Platform Research Laboratory, Fujisawa Pharmaceutical Co., Ibaraki, Japan.
Kita
Y
Y
Morikawa
N
N
Iwami
M
M
Notsu
Y
Y
D000818:Animals; D001127:Arginine Vasopressin / Q000235:genetics*; D001786:Blood Glucose / Q000032:analysis; D015152:Blotting, Northern; D001923:Brain Chemistry; D003920:Diabetes Mellitus / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics; D008099:Liver / Q000737:chemistry; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009994:Osmolar Concentration; D012333:RNA, Messenger / Q000032:analysis*; D017483:Receptors, Vasopressin / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D001786:Blood Glucose; D012333:RNA, Messenger; D017483:Receptors, Vasopressin; D001127:Arginine Vasopressin; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases