Identification of a mutant locus by noncomplementation of a transposon insertion library in Saccharomyces cerevisiae.
We describe a new approach for identifying the gene corresponding to a mutation in Saccharomyces cerevisiae. A library of mTn-lacZ/LEU2 insertions is tested for failure to complement the mutation, and the noncomplementing insertion is used to obtain sequence. This approach offers an alternative to cloning by complementation with a plasmid library.
Wiatrowski
H A
HA
Department of Genetics and Development, Columbia University, New York, New York 10032, USA.
Carlson
M
M
D000483:Alleles; D004247:DNA / Q000378:metabolism; D004251:DNA Transposable Elements / Q000235:genetics*; D004171:Diploidy; D015723:Gene Library; D005816:Genetic Complementation Test; D005821:Genetic Techniques; D009154:Mutation; D016366:Open Reading Frames; D010957:Plasmids / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D004251:DNA Transposable Elements; D004247:DNA