title: [A two-stage technique for producing the microbial exopolysaccaride ethapolan with improved rheological properties].
abstract: A two-stage technique was proposed for cultivating producers of microbial exopolysaccharide ethapolan. The practical value of ethapolan is determined by its rheological properties. The use of a formaldehyde-supplemented medium at the second stage of cultivation improved the rheological properties of ethapolan without reducing its yield. This effect of formaldehyde was due to its binding to the exopolysaccharide, which altered the molecular-mass characteristics of the latter and protected the cells against the toxic action of formaldehyde. At all stages of its purification, ethapolan had improved rheological properties, suggesting that it was tightly bound to formaldehyde.
authors.affiliation: Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, Kiev, 03143 Ukraine.
authors.forename: T P
authors.initials: TP
authors.lastname: Pirog
authors.forename: Iu R
authors.initials: IuR
authors.lastname: Malashenko
authors.forename: S K
authors.initials: SK
authors.lastname: Votselko
mesh_terms: D000150:Acinetobacter / Q000166:cytology / Q000378:metabolism*; D001431:Bacteriological Techniques; D000431:Ethanol / Q000378:metabolism; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification; D012212:Rheology
chemical_list: D011135:Polysaccharides, Bacterial; D000431:Ethanol
vernacular_title: Dvukhstadiĭnyĭ sposob polucheniia mikrobnogo ékzopolisakharida étaponala s uluchshennymi reologicheskimi svoĭstvami.