Development of an effective gene delivery system: a study of complexes composed of a peptide-based amphiphilic DNA compaction agent and phospholipid.
We recently described a basic technology to efficiently combine compacted DNA with phospholipids and hydrophobic peptides, to produce homogenous complexes that are completely resistant to nuclease. We have developed this technology further to form gene delivery complexes that transfect cells effectively in vitro. In addition to plasmid DNA, the complexes contained two basic components: (i) a DNA compacting peptide (-CGKKKFKLKH), either conjugated to lipid or extended to contain (WLPLPWGW-) and (ii) either phosphatidylethanolamine or phosphatidylcholine. Complexes containing a 5.5-fold charge equivalence (peptide charge/DNA charge) of WLPLPWGWCGKKKFKLKH and 5 nmol dimyristoleoylphosphatidylethanolamine/microg DNA produced the highest luciferase gene expression, exceeding 1 x 10(9) relative light units/s/mg protein (>3 microg luciferase per mg protein). These complexes transfected OVCAR-3, COS-7 and HeLa cells at either similar or superior levels when compared to polyethylenimine or lipofectamine complexes. With green fluorescent protein reporter gene, >50% of HeLa cells were positive 30 h after addition of these complexes. Furthermore, these optimal complexes were the least sensitive to pre-treatment of cells with chloroquine, indicating efficient endosomal escape. Our results indicated that self-assembling complexes of plasmid DNA, amphiphilic peptide and phosphatidylethanolamine are highly effective non-viral gene delivery systems.
Murphy
E A
EA
Department of Physiology and Biophysics, College of Medicine, University of California, Irvine, CA 92697-4560, USA. emurphy@uci.edu
Waring
A J
AJ
Murphy
J C
JC
Willson
R C
RC
Longmuir
K J
KJ
D000595:Amino Acid Sequence; D000818:Animals; D019556:COS Cells; D002411:Cation Exchange Resins / Q000737:chemistry; D002738:Chloroquine / Q000494:pharmacology; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D015870:Gene Expression; D020404:Glycerophospholipids / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D008055:Lipids / Q000737:chemistry; D008156:Luciferases / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000737:chemistry; D010713:Phosphatidylcholines / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D010957:Plasmids / Q000737:chemistry / Q000235:genetics; D011094:Polyethyleneimine / Q000737:chemistry; D011993:Recombinant Fusion Proteins / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D014162:Transfection / Q000379:methods*
D002411:Cation Exchange Resins; D020404:Glycerophospholipids; D008055:Lipids; C086724:Lipofectamine; D009842:Oligopeptides; D010713:Phosphatidylcholines; D010743:Phospholipids; D011993:Recombinant Fusion Proteins; C051521:phosphatidylethanol; D002738:Chloroquine; D011094:Polyethyleneimine; D004247:DNA; D008156:Luciferases