[Repair of facial nerve gaps with cryopreserved allografts in rats immunosuppressed with cyclosporin A].
The aim of this study is to assess the feasibility of peripheral nerve allografts pretreated utilizing cold storage and cyclosporin A to improve the facial nerve regeneration in Wistar rat. Three groups were designed: Normal Wistar rats. 4 millimeters nerve gaps repaired with cryopreserved nerve allografts. 4 millimeters nerve gaps repaired with cryopreserved nerve grafts and treated with cyclosporin A. At 16 weeks post-engraftment the animals were evaluated: Facial palsy grading system. Electrophysiologic latency. Axonal counting. Nervous fiber area immediately distal to the graft. The facial function of the experimental groups was similar to the control rats while the latency and morphometric parameters was poor than the normal rats.


CONCLUSIONS
1. Cryopreserved nerve allografts in facial nerve repair in rats are useful in the aquisition of a facial functional nerve recovery as much in immunosuppressed animal as not. 2. Cryopreservation of nerve allografts results a good method of storage of nerve grafts. 3. Cyclosporin A immunosuppression improved not much the grading facial palsy in this model but the electrophysiologic and morphometry is significantly better.
Acuña García
M
M
Cátedra de ORL, Facultad de Medicina, Universidad de Valladolid.
Gil Carcedo García
L M
LM
Alvarez Iscar
N
N
Redondo
L M
LM
Serrat Soto
A
A
Rodríguez Toves
L A
LA
Vaquero Puerta
C
C
D000714:Anastomosis, Surgical / Q000379:methods; D000818:Animals; D015925:Cryopreservation / Q000379:methods*; D016572:Cyclosporine / Q000008:administration & dosage / Q000494:pharmacology*; D005154:Facial Nerve / Q000276:immunology / Q000601:surgery* / Q000648:ultrastructure; D005158:Facial Paralysis / Q000276:immunology / Q000601:surgery*; D005240:Feasibility Studies; D007165:Immunosuppression Therapy / Q000379:methods*; D007166:Immunosuppressive Agents / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D009416:Nerve Regeneration / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D014184:Transplantation, Homologous
D007166:Immunosuppressive Agents; D016572:Cyclosporine
Reparación de lesiones del nervio facial mediante aloinjertos criopreservados en ratas inmunodeprimidas con ciclosporina A.