title: Confirming yeast two-hybrid protein interactions using in vitro glutathione-S-transferase pulldowns.
authors.affiliation: Proctor and Gamble, Cincinnati, OH, USA.
authors.forename: D M
authors.initials: DM
authors.lastname: Kraichely
authors.forename: P N
authors.initials: PN
authors.lastname: MacDonald
mesh_terms: D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics*; D015723:Gene Library; D017930:Genes, Reporter; D005821:Genetic Techniques; D005982:Glutathione Transferase / Q000235:genetics*; D007544:Isopropyl Thiogalactoside / Q000494:pharmacology; D008715:Methionine / Q000378:metabolism; D014176:Protein Biosynthesis; D011993:Recombinant Fusion Proteins / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014158:Transcription, Genetic; D020798:Two-Hybrid System Techniques; D014764:Viral Proteins; D001616:beta-Galactosidase / Q000037:antagonists & inhibitors
chemical_list: D005656:Fungal Proteins; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins; D007544:Isopropyl Thiogalactoside; D008715:Methionine; D005982:Glutathione Transferase; C062210:bacteriophage T7 RNA polymerase; D012321:DNA-Directed RNA Polymerases; D001616:beta-Galactosidase