Cultivation of Polyporus squamosus for pectinase production in aqueous two-phase system containing sugar beet extraction waste.
Cultivation of the fungus Polyporus squamosus for pectinase production was studied in a polyethylene glycol/crude dextran aqueous two-phase system, with sugar beet extraction waste as pectin source. Fungal growth was restricted to the bottom phase and the amounts of biomass and exo-pectinase activity produced were superior to in homogeneous cultivation. The partition coefficients of endo-pectinase and exo-pectinase were 4.26 and 2.78, respectively. The top phase yields in the single extraction step were about 90% for both pectinases.
Antov
M G
MG
Faculty of Technology, Department of Applied Chemistry, University of Novi Sad, Blvd. C. Lazara 1, 21 000, Novi Sad, Yugoslavia. mantov@uns.ns.ac.yu
Pericin
D M
DM
Dimić
G R
GR
D019663:Chenopodiaceae / Q000737:chemistry*; D003470:Culture Media / Q000737:chemistry; D003911:Dextrans / Q000378:metabolism; D005656:Fungal Proteins / Q000096:biosynthesis / Q000302:isolation & purification; D010368:Pectins / Q000378:metabolism; D010936:Plant Extracts / Q000378:metabolism; D011092:Polyethylene Glycols / Q000378:metabolism; D011096:Polygalacturonase / Q000096:biosynthesis* / Q000302:isolation & purification; D011124:Polyporaceae / Q000201:enzymology* / Q000254:growth & development*
D003470:Culture Media; D003911:Dextrans; D005656:Fungal Proteins; D010936:Plant Extracts; D011092:Polyethylene Glycols; D010368:Pectins; D011096:Polygalacturonase