[State of mitochondrial respiration and calcium capacity in livers of rats with different resistance to hypoxia after injections of L-arginine].
In experiments on rats with different resistance to hypoxia are investigated processes of mitochondrial respiration, oxidative phosphorylation and calcium capacity in liver under precursor nitric oxide L-arginine (600 mg/kg) and blockator nitric oxide synthase L-NNA (35 mg/kg) injections. We are used next substrates of oxidation: 0.35 mM succinate, 1 mM alpha-ketoglutarate, 1 mM alpha-ketoglutarate and 2 mM malonic acid. Increasing of ADP-stimulation respiration states under exogenous L-arginine injection, decreasing efficacy of respiration processes (respiration control on Chance and ADP/O) under such substrates oxidation, testify to oxide energy support decreasing and reversing nitric oxide inhibit in such conditions. This will be used as mechanism cell regulation succinate dehydrogenase activity. It has shown that L-arginine injection increase calcium mitochondrial capacity low resistance to hypoxia rats using substrates of oxidation succinate and alpha-ketoglutarate to control meanings of high resistance rats. Effects of nitric oxide precursor influence on this processes limit NO-synthase inhibitor L-NNA.
Kurhaliuk
N M
NM
Ivan Franko Lviv National University.
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000818:Animals; D001120:Arginine / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D000860:Hypoxia / Q000201:enzymology / Q000503:physiopathology; D007656:Ketoglutaric Acids / Q000378:metabolism; D008930:Mitochondria, Liver / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors; D010085:Oxidative Phosphorylation / Q000187:drug effects; D018384:Oxidative Stress; D010101:Oxygen Consumption / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D019802:Succinic Acid / Q000378:metabolism
D004791:Enzyme Inhibitors; D007656:Ketoglutaric Acids; D000244:Adenosine Diphosphate; D001120:Arginine; D019802:Succinic Acid; D019001:Nitric Oxide Synthase; D002118:Calcium
Stan mitokhondrial'noho dykhannia i kal'tsiievoï iemnosti pechinky shchuriv z riznoiu rezystentnistiu do hipoksiï za umov vvedennia L-arhininu.