Activity of fucosyltransferases and altered glycosylation in cystic fibrosis airway epithelial cells.
Cystic fibrosis (CF) glycoconjugates have a glycosylation phenotype of increased fucosylation and/or decreased sialylation when compared with non-CF. A major increase in fucosyl residues linked alpha 1,3 to antennary GlcNAc was observed when surface membrane glycoproteins of CF airway epithelial cells were compared to those of non-CF airway cells. Importantly, the increase in the fucosyl residues was reversed with transfection of CF cells with wild type CFTR cDNA under conditions which brought about a functional correction of the Cl(-) channel defect in the CF cells. In contrast, examination of fucosyl residues in alpha 1,2 linkage by a specific alpha 1,2 fucosidase showed that cell surface glycoproteins of the non-CF cells had a higher percentage of fucose in alpha 1,2 linkage than the CF cells. Airway epithelial cells in primary culture had a similar reciprocal relationship of alpha 1,2- and alpha 1,3-fucosylation when CF and non-CF surface membrane glycoconjugates were compared. In striking contrast, the enzyme activity and the mRNA of alpha 1,2 fucosyltransferase did not reflect the difference in glycoconjugates observed between the CF and non-CF cells. We hypothesize that mutated CFTR may cause faulty compartmentalization in the Golgi so that the nascent glycoproteins encounter alpha 1,3FucT before either the sialyl- or alpha 1,2 fucosyltransferases. In subsequent compartments, little or no terminal glycosylation can take place since the sialyl- or alpha 1,2 fucosyltransferases are unable to utilize a substrate, which is fucosylated in alpha 1,3 position on antennary GlcNAc. This hypothesis, if proven correct, could account for the CF glycophenotype.
Glick
M C
MC
The Cystic Fibrosis Center, Department of Pediatrics, University of Pennsylvania School of Medicine, The Childrens Hospital of Philadelphia, 3516 Civic Center Blvd., ARC 402, Philadelphia, PA 19104-4318, USA. glick@email.chop.edu
Kothari
V A
VA
Liu
A
A
Stoykova
L I
LI
Scanlin
T F
TF
D001980:Bronchi / Q000166:cytology; D002478:Cells, Cultured; D003550:Cystic Fibrosis / Q000378:metabolism*; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000378:metabolism; D004187:Disaccharides / Q000378:metabolism; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism; D005647:Fucosyltransferases / Q000235:genetics / Q000378:metabolism*; D006031:Glycosylation; D006801:Humans; D008562:Membrane Glycoproteins / Q000378:metabolism; D009296:Nasal Cavity / Q000166:cytology; D000097631:Galactoside 2-alpha-L-fucosyltransferase
D019005:Cystic Fibrosis Transmembrane Conductance Regulator; D004187:Disaccharides; D005647:Fucosyltransferases; D008562:Membrane Glycoproteins; D000097631:Galactoside 2-alpha-L-fucosyltransferase; C505032:CFTR protein, human; C112685:fucosyl-1-2-galactose; C106531:3-O-fucopyranosyl-2-acetamido-2-deoxyglucopyranose; C031692:galactoside 3-fucosyltransferase