title: Polymerase chain reaction and denaturing gradient gel electrophoresis monitoring of fecal bifidobacterium populations in a prebiotic and probiotic feeding trial.
abstract: A culture-independent approach based on genus-specific PCR and denaturing gradient gel electrophoresis (DGGE) was used to monitor qualitative changes in fecal bifidobacterial communities in a human feeding trial. DNA was extracted directly from feces and bifidobacterial 16S rDNA sequences were amplified using genus-specific PCR. The PCR fragments were subsequently separated in a sequence-specific manner by DGGE in order to obtain a profile of bifidobacterial fragments. The DGGE profiles revealed that in general, administration for two weeks of galactooligosaccharide and/or Bifidobacterium lactis Bb-12 (8 g and 3 x 10(10) cfu per day, respectively) did not affect the qualitative composition of the indigenous Bifidobacterium population, while B. lactis Bb-12 transiently colonised the gut.
authors.affiliation: Laboratory of Microbiology, Wageningen University, The Netherlands. reetta.satokari@vtt.fi
authors.forename: R M
authors.initials: RM
authors.lastname: Satokari
authors.forename: E E
authors.initials: EE
authors.lastname: Vaughan
authors.forename: A D
authors.initials: AD
authors.lastname: Akkermans
authors.forename: M
authors.initials: M
authors.lastname: Saarela
authors.forename: W M
authors.initials: WM
authors.lastname: De Vos
mesh_terms: D000328:Adult; D001644:Bifidobacterium / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000032:analysis; D004435:Eating; D004587:Electrophoresis, Agar Gel; D005243:Feces / Q000382:microbiology*; D005260:Female; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000008:administration & dosage; D016133:Polymerase Chain Reaction / Q000379:methods*; D019936:Probiotics / Q000008:administration & dosage*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D009844:Oligosaccharides; D012336:RNA, Ribosomal, 16S