Feasibility study on the detection of ferritin using surface plasmon resonance.
Determination of ferritin concentration was performed using surface plasmon resonance technique. An anti-human ferritin antiserum from rabbits was immobilized on the protein-A coated sensor chip. The resonance change due to the binding of ferritin to the antibody was measured. It was found that the antigen-antibody binding of the ferritin could be monitored in real time. The detection of ferritin could be obtained in the range of 25-800 ng/ml. This dynamic range could be applied in various medical applications. Moreover, it was observed that the sensor chips could be regenerated. This offers an opportunity for multiple uses, which will reduce the unit operating cost.
Werawatgoompa
S
S
Department of Biochemistry, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
Sriyudthsak
M
M
D000937:Antigen-Antibody Reactions; D003677:Deficiency Diseases / Q000175:diagnosis*; D005240:Feasibility Studies; D005293:Ferritins / Q000097:blood*; D006801:Humans; D000090463:Iron Deficiencies; D012680:Sensitivity and Specificity; D020349:Surface Plasmon Resonance / Q000379:methods*
D005293:Ferritins