title: E3 ligase activity of RING finger proteins that interact with Hip-2, a human ubiquitin-conjugating enzyme.
abstract: To identify proteins that interact with Huntingtin-interacting protein-2 (Hip-2), a ubiquitin-conjugating enzyme, a yeast two-hybrid screen system was used to isolate five positive clones. Sequence analyses showed that, with one exception, all Hip-2-interacting proteins contained the RING finger motifs. The interaction of Hip-2 with RNF2, one of the clones, was further confirmed through in vitro and in vivo experiments. Mutations in the RING domain of RNF2 prevented the clone from binding to Hip-2, an indication that the RING domain is the binding determinant. RNF2 showed a ubiquitin ligase (E3) activity in the presence of Hip-2, suggesting that a subset of RING finger proteins may have roles as E3s.
authors.affiliation: Graduate School of Biotechnology, Korea University, Seoul, South Korea.
authors.forename: S J
authors.initials: SJ
authors.lastname: Lee
authors.forename: J Y
authors.initials: JY
authors.lastname: Choi
authors.forename: Y M
authors.initials: YM
authors.lastname: Sung
authors.forename: H
authors.initials: H
authors.lastname: Park
authors.forename: H
authors.initials: H
authors.lastname: Rhim
authors.forename: S
authors.initials: S
authors.lastname: Kang
mesh_terms: D000595:Amino Acid Sequence; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D008025:Ligases / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D011485:Protein Binding; D017386:Sequence Homology, Amino Acid; D020798:Two-Hybrid System Techniques; D044763:Ubiquitin-Conjugating Enzymes; D044767:Ubiquitin-Protein Ligases
chemical_list: D004268:DNA-Binding Proteins; C100879:UBE2K protein, human; D044763:Ubiquitin-Conjugating Enzymes; D044767:Ubiquitin-Protein Ligases; D008025:Ligases