[KAISO--a new member of the BTB/POZ family specifically bindsto methylated DNA sequences].
A protein specifically binding a symmetrically methylated DNA fragment of the first intron of the mts1 gene was studied. The protein was purified by gel filtration and affinity chromatography. Mass spectrometry showed that the protein is Kaiso, a new member of the BTB/POZ family. To study the association with methylated DNA sequences in vivo, the location of Kaiso in NIH 3T3 cells was analyzed. Immunofluorescent staining with polyclonal antibodies against Kaiso showed that the protein is predominantly associated with the nucleoli. The causes of its distribution awaits further investigation. The zinc-finger domains of Kaiso were for the first time demonstrated to specifically recognize symmetrically methylated DNA sequences in vitro.
Prokhorchuk
A V
AV
Institute of Gene Biology, Russian Academy of Sciences, Moscow, 117334 Russia. egorbp@dialup.ufg.relarn.ru
Aĭtkhozhina
D S
DS
Sablina
A A
AA
Ruzov
A S
AS
Prokhorchuk
E B
EB
D016475:3T3 Cells; D000818:Animals; D002466:Cell Nucleolus / Q000235:genetics / Q000378:metabolism*; D019175:DNA Methylation; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D019942:Genes, p16; D006801:Humans; D007438:Introns / Q000235:genetics; D051379:Mice; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D011485:Protein Binding; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D016335:Zinc Fingers
D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D014157:Transcription Factors; C492254:ZBTB33 protein, human; C118822:Zbtb33 protein, mouse
KAISO, novyĭ chlen VTV/POZ semeĭstva, spetsifichno sviazyvaetsia s metilirovannymi posledovatel'nostiami DNK.