Use of a cDNA array for the identification of genes induced in islets of suckling rats by a high-carbohydrate nutritional intervention.
Four-day-old rat pups that are raised artificially on a high-carbohydrate (HC) milk formula immediately develop hyperinsulinemia, which persists into adulthood without any further nutritional stimulus. cDNA array analysis was used to identify large-scale changes in gene expression patterns in islets from 12- and 100-day-old HC rats in response to the HC dietary modification during the suckling period. It was observed that the expression of several genes that belong to clusters involved in beta-cell development and/or beta-cell function was significantly upregulated in islets from 12- and 100-day-old HC rats. It is inferred that in addition to predicted changes in gene expression, for example preproinsulin gene, global changes in gene expression contribute to the hyperinsulinemic state in the HC rat.
Song
F
F
Department of Biochemistry, School of Medicine and Biomedical Sciences, State University of New York at Buffalo, Buffalo, New York 14214, USA.
Srinivasan
M
M
Aalinkeel
R
R
Patel
M S
MS
D000375:Aging / Q000502:physiology*; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D000833:Animals, Suckling / Q000502:physiology*; D001402:B-Lymphocytes / Q000502:physiology; D018076:DNA, Complementary / Q000235:genetics; D004040:Dietary Carbohydrates / Q000008:administration & dosage* / Q000494:pharmacology; D018507:Gene Expression Regulation, Developmental; D007515:Islets of Langerhans / Q000187:drug effects / Q000502:physiology*; D020411:Oligonucleotide Array Sequence Analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D015854:Up-Regulation
D018076:DNA, Complementary; D004040:Dietary Carbohydrates