Determinations of morin, quercetin and their conjugate metabolites in serum.
Morin and quercetin are isomeric antioxidant flavonols. High-performance liquid chromatographic methods were developed for the quantitation of morin and quercetin in serum. The method employed a Cosmosil RP-18 column, using acetonitrile/0.2% o-phosphoric acid 28/72 and 27/73 (v/v) as mobile phases, with ethyl paraben and 6,7-dimethoxycoumarin used as internal standards for morin and quercetin, respectively. Moreover, a strategy to stabilize morin/quercetin released from their glucuronides/sulfates in serum during hydrolysis was established. The present methods are applicable for determining morin, quercetin, and their glucuronides/sulfates in serum.
Hsiu
S L
SL
School of Pharmacy, China Medical College, Taichung, Taiwan, ROC.
Tsao
C W
CW
Tsai
Y C
YC
Ho
H J
HJ
Chao
P D
PD
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D005419:Flavonoids / Q000097:blood*; D020719:Glucuronides / Q000097:blood; D006863:Hydrogen-Ion Concentration; D008297:Male; D011794:Quercetin / Q000097:blood*; D011817:Rabbits; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D013431:Sulfates / Q000097:blood; D047309:Flavones
D000975:Antioxidants; D005419:Flavonoids; D020719:Glucuronides; D011794:Quercetin; D013431:Sulfates; C008548:morin; D047309:Flavones