title: Cytokine mRNA levels and lymphocyte infiltration in pancreatic tissue during experimental chronic pancreatitis induced by dibutyltin dichloride.
abstract: There is little information available regarding the role of inflammatory cells in the pathogenesis of chronic pancreatitis. Therefore, we analyzed the local cytokine profile and infiltrating lymphocytes in a rat model of chronic pancreatitis. Experimental pancreatitis was induced by a single intravenous application of dibultyltin dichloride (DBTC). During a time course of two months we observed the mRNA expression of cytokines using competitive RT-PCR. Lymphocytes were characterized by immunohistochemistry, FACS analysis, and the lymphocyte proliferation test. IL-1beta, IL-6, IL-5, and IL-10 were immediately up-regulated in the acute phase of disease, while lymphocyte-restricted expression of IL-2, IL-2R, and IFN-y was only found in the chronic course. Among the infiltrating lymphocytes, CD4+ cells dominated, but during the chronic process there was an increase of CD8+ cells, resulting in a reduced CD4/CD8 ratio. Mitogen-induced activation of isolated mesenteric lymph node cells increased during the chronic inflammation. Our results suggest that in experimental pancreatitis acute inflammatory reactions are followed by a T-lymphocyte-mediated process.
authors.affiliation: Department of Gastroenterology, University of Rostock, Germany.
authors.forename: G
authors.initials: G
authors.lastname: Sparmann
authors.forename: S
authors.initials: S
authors.lastname: Behrend
authors.forename: J
authors.initials: J
authors.lastname: Merkord
authors.forename: H D
authors.initials: HD
authors.lastname: Kleine
authors.forename: E
authors.initials: E
authors.lastname: Graser
authors.forename: T
authors.initials: T
authors.lastname: Ritter
authors.forename: S
authors.initials: S
authors.lastname: Liebe
authors.forename: J
authors.initials: J
authors.lastname: Emmrich
mesh_terms: D000818:Animals; D016516:CD4-CD8 Ratio; D002908:Chronic Disease; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D007150:Immunohistochemistry; D007371:Interferon-gamma / Q000378:metabolism; D007378:Interleukins / Q000378:metabolism; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000378:metabolism / Q000473:pathology*; D008297:Male; D009947:Organotin Compounds; D010179:Pancreas / Q000378:metabolism / Q000473:pathology*; D010195:Pancreatitis / Q000139:chemically induced / Q000276:immunology* / Q000473:pathology; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011917:Rats, Inbred Lew; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013601:T-Lymphocytes / Q000502:physiology; D013997:Time Factors; D015854:Up-Regulation
chemical_list: D016207:Cytokines; D007378:Interleukins; D009947:Organotin Compounds; D012333:RNA, Messenger; D007371:Interferon-gamma; C020735:dibutyldichlorotin