CooA: a heme-containing regulatory protein that serves as a specific sensor of both carbon monoxide and redox state.
CooA, the heme-containing carbon monoxide (CO) sensor from the bacterium Rhodospirillum rubrum, is a transcriptional factor that activates expression of certain genes in response to CO. As with other heme proteins, CooA is unable to bind CO when the Fe heme is oxidized, consistent with the fact that some of the regulated gene products are oxygen-labile. Upon reduction, there is an unusual switch of protein ligands to the six-coordinate heme and the reduced heme is able to bind CO. CO binding stabilizes a conformation of the dimeric protein that allows sequence-specific DNA binding, and transcription is activated through contacts between CooA and RNA polymerase. CooA is therefore a novel redox sensor as well as a specific CO sensor. CooA is a homolog of catabolite responsive protein (CRP), whose transcriptionally active conformation has been known for some time. The recent solution of the crystal structure of the CO-free (transcriptionally inactive) form of CooA has allowed insights into the mechanism by which both proteins respond to their specific small-molecule effectors.
Roberts
G P
GP
Department of Bacteriology, University of Wisconsin-Madison, 53706, USA.
Thorsteinsson
M V
MV
Kerby
R L
RL
Lanzilotta
W N
WN
Poulos
T
T
D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D002248:Carbon Monoxide / Q000378:metabolism*; D004247:DNA / Q000378:metabolism; D029968:Escherichia coli Proteins; D036781:Fimbriae Proteins; D005786:Gene Expression Regulation; D008024:Ligands; D010084:Oxidation-Reduction; D011485:Protein Binding; D011487:Protein Conformation; D012247:Rhodospirillum rubrum / Q000378:metabolism; D015533:Transcriptional Activation / Q000502:physiology
D001426:Bacterial Proteins; C088904:CooA protein, E coli; D029968:Escherichia coli Proteins; D008024:Ligands; D036781:Fimbriae Proteins; D002248:Carbon Monoxide; D004247:DNA