title: Exposure to ultraviolet radiation (290-400 nm) causes oxidative stress, DNA damage, and expression of p53/p73 in laboratory experiments on embryos of the spotted salamander, Ambystoma maculatum.
abstract: Developing embryos of the spotted salamander, Ambystoma maculatum, exposed to ultraviolet radiation (UVR; 290-400 nm) in the laboratory show a significant sensitivity to UVB (290-320 nm) radiation. Embryos in laboratory experiments exhibited significant DNA damage during exposures to UVR despite a significant increase in the production of the protective pigment melanin in response to UVR exposure. DNA damage occurs as a result of both the direct effects of exposure to UVR, and the indirect effects are mediated by the production of reduced oxygen intermediates. The production of reactive oxygen species initiates the expression of p53/p73 that leads to either DNA repair or apoptosis. When similar experiments are conducted on salamander embryos exposed to solar UVR in vernal pools, the embryos show significantly less sensitivity and higher survivorship. The differences between laboratory and field experiments are a result of the attenuation of UVR caused by the accumulation of dissolved organic carbon within the pools of these wooded areas. These findings suggest that northeastern populations of spotted salamanders are sensitive to UVR but are not significantly affected by present-day irradiances of UVR in the field. These results do suggest that continued decreases in stratospheric ozone over temperate latitudes have the potential to affect spotted salamanders in their natural habitats.
authors.affiliation: Department of Zoology, University of New Hampshire, Durham, NH 03824, USA. mpl@christa.unh.edu
authors.forename: M P
authors.initials: MP
authors.lastname: Lesser
authors.forename: S L
authors.initials: SL
authors.lastname: Turtle
authors.forename: J H
authors.initials: JH
authors.lastname: Farrell
authors.forename: C W
authors.initials: CW
authors.lastname: Walker
mesh_terms: D000557:Ambystoma / Q000502:physiology*; D000818:Animals; D004249:DNA Damage; D004268:DNA-Binding Proteins / Q000096:biosynthesis; D004625:Embryo, Nonmammalian; D016147:Genes, Tumor Suppressor; D009687:Nuclear Proteins / Q000096:biosynthesis; D010083:Oxidants, Photochemical; D018384:Oxidative Stress; D010126:Ozone; D000072160:Tumor Protein p73; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis; D025521:Tumor Suppressor Proteins; D014466:Ultraviolet Rays / Q000009:adverse effects*
chemical_list: D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D010083:Oxidants, Photochemical; D000072160:Tumor Protein p73; D016159:Tumor Suppressor Protein p53; D025521:Tumor Suppressor Proteins; D010126:Ozone