A silver staining method for single-cell gel assay.
The single-cell gel assay (comet assay) is a very useful microelectrophoretic technique for evaluation of DNA damage and repair in individual cells. Usually, the comets are visualized and evaluated with fluorescent DNA stains. This staining requires specific equipment (e.g., a high-quality fluorescence microscope), the slides must be analyzed immediately, and they cannot be stored for long periods of time. Here we describe, using human lymphocytes, some modifications of the silver staining for comets that significantly increase the sensitivity/reproducibility of the assay. This silver staining was compared with fluorescence staining and commercial silver stains. (J Histochem Cytochem 49:1183-1186, 2001)
Nadin
S B
SB
Laboratory of Reproduction and Lactation, Regional Center for Scientific and Technological Research, National Research Council of Argentina, Mendoza, Argentina.
Vargas-Roig
L M
LM
Ciocca
D R
DR
D000970:Antineoplastic Agents / Q000494:pharmacology; D002945:Cisplatin / Q000494:pharmacology; D020552:Comet Assay / Q000379:methods*; D004249:DNA Damage; D004317:Doxorubicin / Q000494:pharmacology; D005456:Fluorescent Dyes; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008214:Lymphocytes / Q000648:ultrastructure*; D008297:Male; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D016622:Silver Staining
D000970:Antineoplastic Agents; D005456:Fluorescent Dyes; D004317:Doxorubicin; D006861:Hydrogen Peroxide; D002945:Cisplatin