title: A novel method using baculovirus-mediated gene transfer for production of recombinant adeno-associated virus vectors.
abstract: The baculovirus Autographa californica multiple nucleopolyhedrosis virus causes non-productive infection in mammalian cells. Recombinant baculovirus therefore has the capability to transfer and express heterologous genes in these cells if a mammalian promoter governs the gene of interest. We have investigated the possibility of using baculovirus as a tool to produce recombinant adeno-associated virus (rAAV). AAV has become increasingly popular as a vector for gene therapy and functional genomics efforts, although its use is hampered by the lack of a simple and efficient vector production method. We show here that co-infection of mammalian producer cells with three viruses - a baculovirus containing the reporter gene flanked by AAV ITRs, a baculovirus expressing the AAV rep gene and a helper adenovirus expressing the AAV cap gene - produces infectious rAAV particles. This baculovirus-based chimeric vector method may in future improve large-scale rAAV vector preparations and circumvent present-day problems associated with rAAV production.
authors.affiliation: CNRS UMR 1582, Vectorologie et Transfert de Génes, Institut Gustave Roussy, 94805 Villejuif Cedex, France2.|Center for Genomics Research, Karolinska Institute, SE-17177 Stockholm, Sweden1.
authors.forename: Kerstin
authors.initials: K
authors.lastname: Sollerbrant
authors.affiliation: Center for Genomics Research, Karolinska Institute, SE-17177 Stockholm, Sweden1.
authors.forename: Joacim
authors.initials: J
authors.lastname: Elmén
authors.affiliation: Center for Genomics Research, Karolinska Institute, SE-17177 Stockholm, Sweden1.
authors.forename: Claes
authors.initials: C
authors.lastname: Wahlestedt
authors.affiliation: CNRS UMR 1582, Vectorologie et Transfert de Génes, Institut Gustave Roussy, 94805 Villejuif Cedex, France2.
authors.forename: Joel
authors.initials: J
authors.lastname: Acker
authors.affiliation: CNRS UMR 1582, Vectorologie et Transfert de Génes, Institut Gustave Roussy, 94805 Villejuif Cedex, France2.
authors.forename: Hélene
authors.initials: H
authors.lastname: Leblois-Prehaud
authors.affiliation: CNRS UMR 1582, Vectorologie et Transfert de Génes, Institut Gustave Roussy, 94805 Villejuif Cedex, France2.
authors.forename: Martine
authors.initials: M
authors.lastname: Latta-Mahieu
authors.affiliation: CNRS UMR 1582, Vectorologie et Transfert de Génes, Institut Gustave Roussy, 94805 Villejuif Cedex, France2.
authors.forename: Patrice
authors.initials: P
authors.lastname: Yeh
authors.affiliation: CNRS UMR 1582, Vectorologie et Transfert de Génes, Institut Gustave Roussy, 94805 Villejuif Cedex, France2.
authors.forename: Michel
authors.initials: M
authors.lastname: Perricaudet
mesh_terms: D016367:Baculoviridae / Q000235:genetics*; D002460:Cell Line; D000229:Dependovirus / Q000235:genetics*; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D006801:Humans; D011995:Recombination, Genetic; D014779:Virus Replication