Determination of aniline derivatives in oils related to the toxic oil syndrome by atmospheric pressure ionization-tandem mass spectrometry.
In 1981, an unknown disease appeared in Spain, the Spanish Toxic Oil Syndrome. Nowadays and despite all efforts, the etiological agent is still unknown. Early studies showed a link between this illness and the consumption of denatured rapeseed oil fraudulently processed and marketed as edible oil. Two families of aniline derivatives present in these oils (fatty acid anilides and acylated phenyl amino propanediol derivatives or PAPs) were found to be good chemical markers of toxic oils. In this work, a new method has been developed to analyze these aniline derivatives in oil samples by HPLC-MS and HPLC-MS/MS with an API source. For their quantification, three different internal standards were used, one for anilides and two for PAPs. Quantification limits were 8 ppm for anilides and 0.2 ppm for PAPs. Anilides and PAPs were found in marker-positive samples at levels up to 50,000 and 330 ppm, respectively. The relative abundance of the different fatty acid anilides and PAPs correlates with the fatty acid composition of the oils. More than 2,600 different samples were analyzed by this method in the most exhaustive screening of suspected toxic oils carried out to date.
Calaf
R E
RE
Department of Medical Bioanalysis, IIBB-CSIC, IDIBAPS, Barcelona, Spain.
Peña
J
J
Paytubi
S
S
Blount
B C
BC
Posada de la Paz
M
M
Gelpi
E
E
Abian
J
J
D000814:Aniline Compounds / Q000032:analysis*; D001274:Atmospheric Pressure; D002851:Chromatography, High Pressure Liquid; D005229:Fatty Acids, Monounsaturated; D005506:Food Contamination; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D010938:Plant Oils / Q000737:chemistry* / Q000506:poisoning; D000074262:Rapeseed Oil
D000814:Aniline Compounds; D005229:Fatty Acids, Monounsaturated; D010938:Plant Oils; D000074262:Rapeseed Oil; C023650:aniline