title: High-affinity sodium-vitamin C co-transporters (SVCT) expression in embryonic mouse neurons.
abstract: The sodium-vitamin C co-transporters SVCT1 and SVCT2 transport the reduced form of vitamin C, ascorbic acid. High expression of the SVCT2 has been demonstrated in adult neurons and choroid plexus cells by in situ hybridization. Additionally, embryonic mesencephalic dopaminergic neurons express the SVCT2 transporter. However, there have not been molecular and kinetic analyses addressing the expression of SVCTs in cortical embryonic neurons. In this work, we confirmed the expression of a SVCT2-like transporter in different regions of the fetal mouse brain and in primary cultures of neurons by RT-PCR. Kinetic analysis of the ascorbic acid uptake demonstrated the presence of two affinity constants, 103 microM and 8 microM. A K(m) of 103 microM corresponds to a similar affinity constant reported for SVCT2, while the K(m) of 8 microM might suggest the expression of a very high affinity transporter for ascorbic acid. Our uptake analyses also suggest that neurons take up dehydroascorbic acid, the oxidized form of vitamin C, through the glucose transporters. We consider that the early expression of SVCTs transporters in neurons is important in the uptake of vitamin C, an essential molecule for the fetal brain physiology. Vitamin C that is found at high concentration in fetal brain may function in preventing oxidative free radical damage, because antioxidant radical enzymes mature only late in the developing brain.
authors.affiliation: Department of Embryology, Faculty of Biological Sciences, University of Concepción, Concepción, Chile.
authors.forename: M
authors.initials: M
authors.lastname: Castro
authors.forename: T
authors.initials: T
authors.lastname: Caprile
authors.forename: A
authors.initials: A
authors.lastname: Astuya
authors.forename: C
authors.initials: C
authors.lastname: Millán
authors.forename: K
authors.initials: K
authors.lastname: Reinicke
authors.forename: J C
authors.initials: JC
authors.lastname: Vera
authors.forename: O
authors.initials: O
authors.lastname: Vásquez
authors.forename: L G
authors.initials: LG
authors.lastname: Aguayo
authors.forename: F
authors.initials: F
authors.lastname: Nualart
mesh_terms: D000818:Animals; D001205:Ascorbic Acid / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology; D003571:Cytochalasin B / Q000494:pharmacology; D003572:Cytochalasins / Q000494:pharmacology; D004622:Embryo, Mammalian / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D007150:Immunohistochemistry; D007700:Kinetics; D051379:Mice; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000378:metabolism*; D029363:Organic Anion Transporters, Sodium-Dependent; D010042:Ouabain / Q000494:pharmacology; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012964:Sodium / Q000378:metabolism; D060347:Sodium-Coupled Vitamin C Transporters; D027981:Symporters
chemical_list: D002352:Carrier Proteins; D003572:Cytochalasins; D004791:Enzyme Inhibitors; D029363:Organic Anion Transporters, Sodium-Dependent; D011506:Proteins; C555164:Slc23a1 protein, mouse; C555165:Slc23a2 protein, mouse; D060347:Sodium-Coupled Vitamin C Transporters; D027981:Symporters; C007215:cytochalasin E; D003571:Cytochalasin B; D010042:Ouabain; D012964:Sodium; D001205:Ascorbic Acid