title: Association of induced disease resistance of rhododendron seedlings with inoculation of Streptomyces sp. R-5 and treatment with actinomycin D and amphotericin B to the tissue-culture medium.
abstract: Seedlings of rhododendron were treated by adding Streptomyces sp. strain R-5, actinomycin D and/or amphotericin B to the tissue-culture medium. HPLC analysis showed that all of the treated seedlings contained these antibiotics at concentrations higher than the suppressive levels to mycelial growth of Pestalotiopsis sydowiana, a major pathogen of rhododendron. Occurrence of disease caused by this fungus in the seedlings was suppressed by treatment of the medium surface with strain R-5, but not by treatment with these antibiotics, suggesting that growth of strain R-5, an antibiotic producer, could be essential for induction of disease resistance in tissue-cultured seedlings of rhododendron.
authors.affiliation: Laboratory of Ecological Circulation, Faculty of Bioresources, Mie University, Tsu-city, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Shimizu
authors.forename: T
authors.initials: T
authors.lastname: Furumai
authors.forename: Y
authors.initials: Y
authors.lastname: Igarashi
authors.forename: H
authors.initials: H
authors.lastname: Onaka
authors.forename: T
authors.initials: T
authors.lastname: Nishimura
authors.forename: R
authors.initials: R
authors.lastname: Yoshida
authors.forename: H
authors.initials: H
authors.lastname: Kunoh
mesh_terms: D000666:Amphotericin B / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000935:Antifungal Agents / Q000494:pharmacology*; D046508:Culture Techniques; D003609:Dactinomycin / Q000494:pharmacology*; D019684:Magnoliopsida / Q000187:drug effects* / Q000382:microbiology*; D003904:Mitosporic Fungi; D009181:Mycoses / Q000188:drug therapy / Q000276:immunology / Q000517:prevention & control; D010935:Plant Diseases / Q000382:microbiology*; D013302:Streptomyces / Q000737:chemistry* / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D003609:Dactinomycin; D000666:Amphotericin B