cDNA cloning and molecular identification of the major oyster allergen from the Pacific oyster Crassostrea gigas.
BACKGROUND
Shellfish is one of the most common food allergens. Despite the recent cloning and molecular identification of the major heat stable crustacean allergens in shrimp, lobster and crab, there have been no similar studies on molluscs to which a significant portion of populations allergic to shellfish are also hypersensitive. Recent biochemical evidence suggests that tropomyosin is also an allergen in molluscs, but data on the molecular cloning, nucleotide sequencing, expression and IgE binding to mollusc tropomyosin are lacking.


OBJECTIVE
This study was undertaken to clone, identify and determine the primary structure of a major IgE-reactive mollusc allergen in oyster at the DNA and protein level.


METHODS
We constructed an expression cDNA library from the Pacific oyster Crassostrea gigas. This library was screened for IgE binding clones using sera from 15 subjects with a well-documented history of type I hypersensitivity reactions to oysters. An IgE reactive clone was selected and sub-cloned into plasmids for nucleotide sequence determination and expression in E. coli.


RESULTS
We identified a 1.3-kb cDNA designated as Cra g 1.03. Expression of Cra g 1.03 in plasmid vector pGEX produced a 59-kDa recombinant fusion protein reactive to the IgE antibodies from patients with oyster allergies but not non-allergic controls. Cra g 1.03 has an open reading frame of 233 amino acids and demonstrates marked similarity in amino acid composition and peptide sequence with mollusc and crustacean tropomyosins. Absorption of oyster allergic sera with Cra g 1.03 totally removed IgE reactivity to oyster extract. Moreover, absorption of allergic sera with recombinant shrimp tropomyosin (Met e 1), lobster tropomyosin (Pan s 1) and crab tropomyosin (Cha f 1) removed most of the IgE reactivity to Cra g 1.03.


CONCLUSIONS
Cra g 1.03 is the first oyster allergen identified at the molecular level. Nucleotide and amino acid comparison shows that this protein is the oyster tropomyosin.
Leung
P S
PS
Division of Rheumatology/Allergy and Clinical Immunology, School of Medicine, University of California, Davis, CA 95616, USA. psleung@ucdavis.edu
Chu
K H
KH
D000485:Allergens / Q000235:genetics* / Q000302:isolation & purification*; D000595:Amino Acid Sequence / Q000235:genetics; D000818:Animals; D052179:Antigens, Plant; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D000939:Epitopes / Q000276:immunology; D007073:Immunoglobulin E / Q000276:immunology / Q000378:metabolism; D008969:Molecular Sequence Data; D010125:Ostreidae / Q000235:genetics* / Q000276:immunology*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000276:immunology / Q000302:isolation & purification; D016415:Sequence Alignment; D014335:Tropomyosin / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification
D000485:Allergens; D052179:Antigens, Plant; C436957:Cra g 1.03 allergen; D018076:DNA, Complementary; D000939:Epitopes; D011994:Recombinant Proteins; D014335:Tropomyosin; D007073:Immunoglobulin E