Biogenesis of Golgi stacks in imaginal discs of Drosophila melanogaster.
We provide a detailed description of Golgi stack biogenesis that takes place in vivo during one of the morphogenetic events in the lifespan of Drosophila melanogaster. In early third-instar larvae, small clusters consisting mostly of vesicles and tubules were present in epithelial imaginal disk cells. As larvae progressed through mid- and late-third instar, these larval clusters became larger but also increasingly formed cisternae, some of which were stacked. In white pupae, the typical Golgi stack was observed. We show that larval clusters are Golgi stack precursors by 1) localizing various Golgi-specific markers to the larval clusters by electron and immunofluorescence confocal microscopy, 2) driving this conversion in wild-type larvae incubated at 37 degrees C for 2 h, and 3) showing that this conversion does not take place in an NSF1 mutant (comt 17). The biological significance of this conversion became clear when we found that the steroid hormone 20-hydroxyecdysone (ecdysone) is critically involved in this conversion. In its absence, Golgi stack biogenesis did not occur and the larval clusters remained unaltered. We showed that dGM130 and sec23p expression increases approximately three- and fivefold, respectively, when discs are exposed to ecdysone in vivo and in vitro. Taken together, these results suggest that we have developed an in vivo system to study the ecdysone-triggered Golgi stack biogenesis.
Kondylis
V
V
The Wellcome Trust Centre for Cell Biology, Institute of Cell and Molecular Biology, University of Edinburgh, Edinburgh, EH9 3JR, Scotland, United Kingdom.
Goulding
S E
SE
Dunne
J C
JC
Rabouille
C
C
D000818:Animals; D030801:Animals, Genetically Modified; D001324:Autoantigens; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism; D004331:Drosophila melanogaster / Q000254:growth & development* / Q000502:physiology / Q000648:ultrastructure; D004440:Ecdysone / Q000235:genetics / Q000494:pharmacology; D005260:Female; D006056:Golgi Apparatus / Q000737:chemistry / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D006801:Humans; D015151:Immunoblotting; D007814:Larva / Q000254:growth & development / Q000502:physiology / Q000648:ultrastructure; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D008856:Microscopy, Fluorescence; D009024:Morphogenesis; D017351:N-Acetylglucosaminyltransferases / Q000378:metabolism; D052066:N-Ethylmaleimide-Sensitive Proteins; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D014020:Tissue Extracts / Q000737:chemistry; D033921:Vesicular Transport Proteins
D001324:Autoantigens; D002352:Carrier Proteins; D029721:Drosophila Proteins; C097741:Golgin subfamily A member 2; D008565:Membrane Proteins; D010750:Phosphoproteins; D011993:Recombinant Fusion Proteins; C106113:SEC31 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014020:Tissue Extracts; D033921:Vesicular Transport Proteins; C498732:comt protein, Drosophila; D004440:Ecdysone; D017351:N-Acetylglucosaminyltransferases; C090576:fng protein, Drosophila; D052066:N-Ethylmaleimide-Sensitive Proteins