Template images for nonhuman primate neuroimaging: 1. Baboon.
Coregistration of functional brain images across many subjects offers several experimental advantages and is widely used for studies in humans. Voxel-based coregistration methods require a high-quality 3-D template image, preferably one that corresponds to a published atlas. Template images are available for human, but we could not find an appropriate template for neuroimaging studies in baboon. Here we describe the formation of a T1-weighted structural MR template image and a PET blood flow template, derived from 9 and 7 baboons, respectively. Custom software aligns individual MR images to the MRI template; human supervision is needed only to initially estimate any gross rotational misalignment. In these aligned individual images, internal subcortical fiducial points correspond closely to a photomicrographic baboon atlas with an average error of 1.53 mm. Cortical test points showed a mean error of 1.99 mm compared to the mean location for each point. Alignment of individual PET blood flow images directly to the PET template was compared to a two-step alignment process via each subject's MR image. The two transformations were identical within 0.41 mm, 0.54 degrees, and 1.0% (translation, rotation, and linear stretch; mean). These quantities provide a check on the validity of the alignment software as well as of the template images. The baboon structural MR and blood flow PET templates are available on the Internet (purl.org/net/kbmd/b2k) and can be used as targets for any image registration software.
Black
K J
KJ
Department of Psychiatry, Washington University School of Medicine, St. Louis, Missouri 63110, USA. kevin@npg.wustl.edu
Snyder
A Z
AZ
Koller
J M
JM
Gado
M H
MH
Perlmutter
J S
JS
D000818:Animals; D001921:Brain / Q000000981:diagnostic imaging / Q000502:physiology*; D001931:Brain Mapping / Q000379:methods; D002560:Cerebrovascular Circulation; D005260:Female; D021621:Imaging, Three-Dimensional / Q000379:methods* / Q000592:standards; D008279:Magnetic Resonance Imaging; D008297:Male; D010215:Papio / Q000502:physiology*; D012984:Software / Q000592:standards; D014055:Tomography, Emission-Computed