Mapping the ligand binding site at protein side-chains in protein-ligand complexes through NOE difference spectroscopy.
This report describes a novel NMR approach for mapping the interaction surface between an unlabeled ligand and a 13C,15N-labeled protein. The method relies on the spin inversion properties of the dipolar relaxation pathways and records the differential relaxation of two spin modes, where ligand and protein 1H magnetizations are aligned either in a parallel or anti-parallel manner. Selective inversion of protein protons is achieved in a straightforward manner by exploiting the one-bond heteronuclear scalar couplings (1J(CH), 1J(NH)). Suppression of indirect relaxation pathways mediated by bulk water or rapidly exchanging protons is achieved by selective inversion of the water signal in the middle of the NOESY mixing period. The method does not require deuteration of the protein or well separated spectral regions for the protein and the ligand, respectively. Additionally, in contrast to previous methods, the new experiment identifies side-chain enzyme ligand interactions along the intermolecular binding interface. The method is demonstrated with an application to the B12-binding subunit of glutamate mutase from Clostridium tetanomorphum for which NMR chemical shift changes upon B12-nucleotide loop binding and a high-resolution solution structure are available.
Eichmüller
C
C
Institute of Organic Chemistry, University of Innsbruck, Austria.
Tollinger
M
M
Kräutler
B
B
Konrat
R
R
D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism; D001665:Binding Sites; D002247:Carbon Isotopes / Q000032:analysis; D003013:Clostridium / Q000201:enzymology; D003067:Coenzymes / Q000737:chemistry / Q000378:metabolism; D019751:Intramolecular Transferases / Q000737:chemistry / Q000378:metabolism; D008024:Ligands; D046911:Macromolecular Substances; D008958:Models, Molecular; D009587:Nitrogen Isotopes / Q000032:analysis; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism; D014805:Vitamin B 12 / Q000737:chemistry / Q000378:metabolism
D001426:Bacterial Proteins; D002247:Carbon Isotopes; D003067:Coenzymes; D008024:Ligands; D046911:Macromolecular Substances; D009587:Nitrogen Isotopes; D011506:Proteins; D019751:Intramolecular Transferases; C021570:methylaspartate mutase; D014805:Vitamin B 12