Separation of mistletoe lectins based on the degree of glycosylation using boronate affinity chromatography.
A mixture of two mistletoe lectins (MLs) has been separated according to the degree of glycosylation using boronate affinity chromatography. The mistletoe lectins, mistletoe lectin I (MLI) and mistletoe lectin III (MLIII) with degrees of glycosylation of 6.1 and 3.8%, respectively, were used in the investigation. MLI exhibited a higher retention time than MLIII due to its higher degree of glycosylation. Separation was confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The developed method may lead to new applications for the boronate affinity technique, as well as provide an alternative separation method for MLs.
Li
Y
Y
Gramineer International AB, IDEON, Lund, Sweden.
Pfüller
U
U
Larsson
E L
EL
Jungvid
H
H
Galaev IYu
Mattiasson
B
B
D001897:Boronic Acids / Q000737:chemistry*; D002846:Chromatography, Affinity / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D006031:Glycosylation; D028321:Plant Preparations; D010940:Plant Proteins; D054790:Ribosome Inactivating Proteins, Type 2; D012685:Sepharose; D014118:Toxins, Biological / Q000737:chemistry / Q000302:isolation & purification*
D001897:Boronic Acids; D028321:Plant Preparations; D010940:Plant Proteins; D054790:Ribosome Inactivating Proteins, Type 2; D014118:Toxins, Biological; C009674:ribosome inactivating protein, Viscum; D012685:Sepharose