Expression profiling of cancer-related genes in human keratinocytes following non-lethal ultraviolet B irradiation.
Ultraviolet B irradiation initiates and promotes skin cancers, photo-aging, and immune suppression. In order to elucidate the effect of these processes at the level of gene expression, we used cDNA microarray technology to examine the effect of ultraviolet B irradiation on 588 cancer-related genes in human keratinocytes at 1, 6, and 24 h post-irradiation with a mildly cytotoxic dose of ultraviolet B (170 mJ/cm(2)). The viability of the irradiated keratinocytes was 75% at 24 h post-irradiation. Various cytokeratins and transcription factors were up-regulated within 1 h post-irradiation. After 6 h, expression of a variety of genes related to growth regulation (e.g. p21(WAF1), notch 4, and smoothened), apoptosis (e.g. caspase 10, hTRIP, and CRAF1), DNA repair (ERCC1, XRCC1), cytokines (e.g. IL-6, IL-13, TGF-beta, and endothelin 2), and cell adhesion (e.g. RhoE, and RhoGDI) were altered in human keratinocytes. These data suggest the changes in a cascade of gene expression in human keratinocytes occurring within 24 h after UVB exposure. Although the roles of these cellular genes after UVB-irradiation remain to be elucidated, microarray analysis may provide a new view of gene expression in epidermal keratinocytes following UVB exposure.
Murakami
T
T
Department of Dermatology, Jichi Medical School, 3311-1 Yakushiji, Minamikawachi-machi, Kawachi-gun, Tochigi 329-0498, Japan. takmu@jichi.ac.jp
Fujimoto
M
M
Ohtsuki
M
M
Nakagawa
H
H
D017209:Apoptosis; D002470:Cell Survival / Q000528:radiation effects; D002471:Cell Transformation, Neoplastic; D016207:Cytokines / Q000235:genetics; D004260:DNA Repair; D018076:DNA, Complementary; D004307:Dose-Response Relationship, Radiation; D016232:Endothelins / Q000235:genetics; D005786:Gene Expression Regulation / Q000528:radiation effects*; D006133:Growth Substances / Q000235:genetics; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000502:physiology / Q000528:radiation effects*; D007633:Keratins / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics; D014158:Transcription, Genetic / Q000528:radiation effects; D014466:Ultraviolet Rays
D016207:Cytokines; D018076:DNA, Complementary; D016232:Endothelins; D006133:Growth Substances; D012333:RNA, Messenger; D014157:Transcription Factors; D007633:Keratins