Interaction between ATBP and DmUbc9 in the expression of the Sarcophaga lectin gene.
We previously demonstrated that (A+T)-stretch binding protein (ATBP) and Dorsal-related immunity factor (Dif) are required for the expression of the Sarcophaga lectin gene in SL-2 cells (Aozasa et al., Eur. J. Biochem. 268, 2506-2511, 2001). The present study demonstrates that DmUbc9 interacts with ATBP, and cotransfection of the DmUbc9 vector with ATBP and Dif vectors greatly enhances the expression of the luciferase reporter of the Sarcophaga lectin gene in SL-2 cells. These results suggest that sumoylation of ATBP is involved in the expression of the Sarcophaga lectin gene in this system.
Aozasa
N
N
Graduate School of Pharmaceutical Sciences, University of Tokyo, Bunkyo-ku, Tokyo, 113-0033, Japan.
Shiraishi
H
H
Kobayashi
A
A
Sekimizu
K
K
Kubo
T
T
Natori
S
S
D000818:Animals; D001665:Binding Sites; D002460:Cell Line; D018076:DNA, Complementary / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000378:metabolism*; D004175:Diptera; D029721:Drosophila Proteins; D015723:Gene Library; D017930:Genes, Reporter; D005822:Genetic Vectors; D005982:Glutathione Transferase / Q000378:metabolism; D019476:Insect Proteins; D037102:Lectins / Q000737:chemistry / Q000378:metabolism; D008156:Luciferases / Q000378:metabolism; D009687:Nuclear Proteins / Q000737:chemistry* / Q000378:metabolism*; D011485:Protein Binding; D011994:Recombinant Proteins / Q000378:metabolism; D014158:Transcription, Genetic; D014162:Transfection; D020798:Two-Hybrid System Techniques
C094603:ATBP protein, Sarcophaga peregrina; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D019476:Insect Proteins; D037102:Lectins; D009687:Nuclear Proteins; D011994:Recombinant Proteins; D008156:Luciferases; D005982:Glutathione Transferase