Expression of Clarkia S-linalool synthase in transgenic petunia plants results in the accumulation of S-linalyl-beta-D-glucopyranoside.
Petunia hybrida W115 was transformed with a Clarkia breweri S-linalool synthase cDNA (lis). Lis was expressed in all tissues analysed, and linalool was detected in leaves, sepals, corolla, stem and ovary, but not in nectaries, roots, pollen and style. However, the S-linalool produced by the plant in the various tissues is not present as free linalool, but was efficiently converted to non-volatile S-linalyl-beta-D-glucopyranoside by the action of endogenous glucosyltransferase. The results presented demonstrate that monoterpene production can be altered by genetic modification, and that the compounds produced can be converted by endogenous enzymatic activity.
Lücker
J
J
Business Unit Cell Cybernetics, Plant Research International, PO Box 16, 6700 AA, Wageningen, The Netherlands.
Bouwmeester
H J
HJ
Schwab
W
W
Blaas
J
J
van der Plas
L H
LH
Verhoeven
H A
HA
D000080462:Acyclic Monoterpenes; D002853:Chromatography, Liquid; D018076:DNA, Complementary; D005960:Glucosides / Q000378:metabolism*; D006836:Hydro-Lyases / Q000235:genetics / Q000378:metabolism; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D039821:Monoterpenes; D018515:Plant Leaves / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D019685:Rosales / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D013729:Terpenes / Q000378:metabolism*
D000080462:Acyclic Monoterpenes; D018076:DNA, Complementary; D005960:Glucosides; D039821:Monoterpenes; C438307:S-linalyl-beta-D-glucopyranoside; D013729:Terpenes; C018584:linalool; D006836:Hydro-Lyases; C092055:S-linalool synthase