[Differential decondensation of mitotic chromosomes in SPEV tissue cultured cells induced by repeated hypotonic shock ].
A new method of differential decondensation of mitotic chromosomes has been proposed by means of repeated treatment of live cells with 15% Hanks' balanced salt solution. The procedure of cell treatment includes three stages: the first hypotonic shock, cultivation in isotonic medium, and the second hypotonic shock. As a result, after a standard methanol-acetic acid fixation and Giemsa staining some discrete Giemsa-positive globules are revealed in mitotic chromosomes. Such globules are symmetrically arranged in axial regions of sister chromatids. The comparative analysis of marker chromosomes has revealed a topological conformity of these globules to G-bands of chromosomes. It has been shown that it is the first hypotonic shock that triggers induction of structural modification of chromatin in interphase nuclei and in mitotic chromosomes. Of interest is the fact that the effect of the first shock is prolonged in time and is realized during at least one cell cycle, with the normal structure of mitotic chromosomes being restored after S-phase of the successive cell cycle.
Kobliakova
Iu V
IuV
A. N. Belozersky Laboratory of Molecular Biology and Bioorganic Chemistry, Moscow State University, Moscow 119899.
Kireev
I I
II
Stefanova
V N
VN
Zatsepina
O V
OV
Poliakov
V Iu
VIu
D000818:Animals; D001399:Azure Stains; D002460:Cell Line; D002875:Chromosomes / Q000502:physiology / Q000648:ultrastructure*; D007038:Hypotonic Solutions; D008938:Mitosis; D013552:Swine
D001399:Azure Stains; D007038:Hypotonic Solutions
Differentsial'naia dekondensatsiia mitoticheskikh khromosom v kletkakh kul'tury tkani SPEV, indutsirovannaia dvukratnym gipotonicheskim shokom.