Glycine reduces the inflammatory response and organ damage in a two-hit sepsis model in rats.
The goal of this study was to investigate whether prefeeding of glycine reduces the immunoinflammatory response, the degree of distant organ injury (liver), and/or the mortality rate in a two-hit model using intestinal ischemia/reperfusion and endotoxin (ET) challenge 6 h later in rats. The liver damage was greatest at 24 h after ET challenge and completely inhibited by glycine. The early systemic increase of the proinflammatory cytokines tumor necrosis factor-alpha (TNF-alpha) and interleukin (IL) -6 as well as the secretion of the antiinflammatory cytokine IL-10 was reduced by glycine. Tissue cytokine mRNA expression (TNF-alpha, IL-1beta, IL-10) was decreased in the lung and the liver but not in the mesenteric lymph node or ileum, in the glycine-fed group. However, glycine did not decrease the mortality rate. These results suggest that prefeeding of glycine reduces liver damage as well as the systemic and local (lung and liver) inflammatory response after intestinal ischemia/reperfusion and endotoxin challenge in rats.
Grotz
M R
MR
Department of Trauma Surgery, Hannover Medical School, Germany.
Pape
H C
HC
van Griensven
M
M
Stalp
M
M
Rohde
F
F
Bock
D
D
Krettek
C
C
D000596:Amino Acids / Q000097:blood; D000818:Animals; D016207:Cytokines / Q000097:blood / Q000235:genetics; D004195:Disease Models, Animal; D004731:Endotoxins / Q000633:toxicity; D005998:Glycine / Q000097:blood / Q000494:pharmacology* / Q000627:therapeutic use; D007249:Inflammation / Q000517:prevention & control*; D008111:Liver Function Tests; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D018805:Sepsis / Q000097:blood / Q000188:drug therapy / Q000401:mortality / Q000503:physiopathology*; D014158:Transcription, Genetic; D015430:Weight Gain
D000596:Amino Acids; D016207:Cytokines; D004731:Endotoxins; D005998:Glycine