18O pattern and biosynthesis of natural plant products.
Oxygen atoms in plant products originate from CO(2), H(2)O and O(2), precursors with quite different delta18O values. Furthermore their incorporation by different reactions implies isotope effects. On this base the resulting non-statistical 18O distributions in natural compounds are discussed. The delta18O value of cellulose is correlated to that of the leaf water, and the observed 18O enrichment (approximately +27 per thousand) is generally attributed to an equilibrium isotope effect between carbonyl groups and water. However, as soluble and heterotrophically synthesised carbohydrates show other correlations, a non-statistical 18O distribution - originating from individual biosynthetic reactions - is postulated for carbohydrates. Similarly, the delta18O values of organic acids, carbonyl compounds, alcohols and esters indicate water-correlated, but individual 18O abundances (e.g. O from acyl groups approximately +19% above water), depending upon origin and biosyntheses. Alcoholic groups introduced by monooxygenase reactions, e.g. in sterols and phenols, show delta18O values near +5 per thousand, in agreement with an assumed isotope fractionation factor of approximately 1.02 on the reaction with atmospheric oxygen (delta18O=+23.5 per thousand). Correspondingly, a "thermodynamically ordered isotope distribution" is only observed for oxygen in some functional groups correlated to an origin from CO(2) and H(2)O, not from O(2). The individual isotopic increments of functional groups permit the prediction of global delta18O values of natural compounds on the basis of their biosynthesis.
Schmidt
H L
HL
Technische Universität München, Lehrstuhl für Biologische Chemie, Vöttinger Str. 40, D-85350 Freising-Weihenstephan, Germany. hlschmidt@nikocity.de
Werner
R A
RA
Rossmann
A
A
D000438:Alcohols / Q000378:metabolism; D001685:Biological Factors / Q000096:biosynthesis*; D002241:Carbohydrates / Q000096:biosynthesis; D002264:Carboxylic Acids / Q000378:metabolism; D004952:Esters / Q000378:metabolism; D007700:Kinetics; D010103:Oxygen Isotopes / Q000378:metabolism; D018515:Plant Leaves / Q000378:metabolism; D010944:Plants / Q000378:metabolism*; D014867:Water
D000438:Alcohols; D001685:Biological Factors; D002241:Carbohydrates; D002264:Carboxylic Acids; D004952:Esters; D010103:Oxygen Isotopes; D014867:Water