Identification of 1-adenine DNA adducts in workers occupationally exposed to styrene.
Styrene is an extensively used industrial chemical that has been classified as a possible human carcinogen. The possible carcinogenicity may be related to the covalent DNA binding properties of styrene 7,8-oxide, a major metabolite of styrene. We have developed a sensitive and a highly specific phosphorus-32-postlabeling method for the determination of 1-styrene 7,8-oxide-adenine DNA adducts. These adducts were analyzed in white blood cells from workers exposed to styrene at mean level of 76.2 mg/m3. Three of nine exposed workers showed adducts above the detection limit; the mean was 0.79 +/- 0.14 1-styrene 7,8-oxide-adenine DNA adducts/10(9) nucleotides. None of the 11 control cells showed adducts above the detection limit, which was 0.4 adducts/10(9) nucleotides. The results show a potential of 1-adenine DNA adducts for predicting risks in the workers exposed to styrene.
Koskinen
M
M
Department of Biosciences at Novum, Karolinska Institute, S-14157 Huddinge, Sweden.
Vodicka
P
P
Hemminki
K
K
D000225:Adenine / Q000097:blood; D000328:Adult; D000395:Air Pollutants, Occupational / Q000032:analysis; D000818:Animals; D015415:Biomarkers; D018736:DNA Adducts / Q000032:analysis; D004784:Environmental Monitoring; D005260:Female; D006801:Humans; D008297:Male; D008333:Mandelic Acids / Q000652:urine; D051379:Mice; D008875:Middle Aged; D016273:Occupational Exposure / Q000009:adverse effects; D009790:Occupations; D051381:Rats; D012680:Sensitivity and Specificity; D012907:Smoking / Q000009:adverse effects; D020058:Styrene / Q000009:adverse effects* / Q000032:analysis / Q000097:blood / Q000378:metabolism; D013997:Time Factors
D000395:Air Pollutants, Occupational; D015415:Biomarkers; D018736:DNA Adducts; D008333:Mandelic Acids; D020058:Styrene; D000225:Adenine; C037938:mandelic acid