On-line immunoaffinity extraction followed by high-performance liquid chromatography and radioimmunoassay for a novel retinobenzoic acid, AM-80, in human plasma.
On-line coupled immunoaffinity chromatography and reversed-phase high performance liquid chromatography (on-line IAC-HPLC) with detection by radioimmunoassay (RIA) was developed for 4-[(5,6,7,8-tetrahydro-5,5,8,8-tetramethyl-2-naphthyl)-carbamoyl] benzoic acid (Am-80) in human plasma. A 0.05-mL sample was directly loaded onto an immunoaffinity pre-column packed with immobilized polyclonal antibodies against Am-80. The immunoaffinity extract was then automatically introduced to reversed-phase
Itoh
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M
Shionogi Research Laboratories, Shionogi & Co, Ltd, Toyonaka, Osaka, Japan.
Kominami
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G
D001565:Benzoates / Q000097:blood*; D002846:Chromatography, Affinity / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D011863:Radioimmunoassay / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013764:Tetrahydronaphthalenes / Q000097:blood*
D001565:Benzoates; D013764:Tetrahydronaphthalenes; C061133:tamibarotene