Pyrokinin/PBAN radio-receptor assay: development and application for the characterization of a putative receptor from the pheromone gland of Heliothis peltigera.
A radio-receptor assay (RRA) for the insect pyrokinin/PBAN family has been developed. The development involved examination of the ligand (3H-tyrosyl-PBAN28-33NH2)-receptor interaction under various incubation conditions and variations on sex pheromone gland membrane preparation. Application of the RRA for a partial characterization of the putative pyrokinin/PBAN receptor in the pheromone gland of H. peltigera revealed age-dependence of its expression. Pharmacological characterization revealed a high correlation between the binding-affinity to the receptor of various PBAN-derived peptides and their in vivo pheromonotropic bioactivity, and shed light on the interaction of backbone cyclic and linear ([Arg27,D-Phe30]PBAN28-33NH2) PBAN antagonists with the receptor.
Altstein
M
M
Department of Entomology, The Volcani Center, 50250, Bet Dagan, Israel. vinnie2@netvision.net.il
Ben-Aziz
O
O
Daniel
S
S
Zeltser
I
I
Gilon
C
C
D000818:Animals; D001665:Binding Sites; D001667:Binding, Competitive; D002021:Buffers; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002628:Chemoreceptor Cells / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D005260:Female; D006863:Hydrogen-Ion Concentration; D007301:Insect Hormones / Q000737:chemistry / Q000378:metabolism; D008024:Ligands; D009036:Moths; D009479:Neuropeptides / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D010456:Peptides, Cyclic / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism*; D010675:Pheromones / Q000378:metabolism*; D011480:Protease Inhibitors / Q000378:metabolism; D011869:Radioligand Assay / Q000379:methods*; D013329:Structure-Activity Relationship
D002021:Buffers; D007301:Insect Hormones; D008024:Ligands; D009479:Neuropeptides; D010456:Peptides, Cyclic; D010675:Pheromones; D011480:Protease Inhibitors; C079229:pyrokinin; C060896:pheromone-biosynthesis-activating neuropeptide I