Altered dopaminergic transmission in the anorexic anx/anx mouse striatum.
We demonstrate abnormal dopaminergic neurotransmission in anorexic mice, homozygous for a recessive mutation (anx) causing starvation and motor disturbances. Isolated neurons from anx/anx striatum displayed a markedly increased activity of the Na+,K+-ATPase compared with normal littermates. Dopamine down-regulates Na+,K+-ATPase activity in striatal medium spiny neurons in rat, mouse and guinea pig. However, addition of dopamine in vitro failed to suppress the increased activity in anx/anx striatal neurons. Striatal dopamine and its metabolites, but not norepinephrine, were slightly but significantly lower in anx/anx mice than in normal littermates. We suggest that abnormal dopaminergic transmission may contribute to the anx phenotype.
Johansen
J E
JE
Department of Molecular Medicine, Karolinska Institutet, Karolinska Hospital, 171 76 Stockholm, Sweden. jeanette.johansen@cnm.ki.se
Teixeira
V L
VL
Johansson
C
C
Serrão
P
P
Berggren
P O
PO
Soares-Da-Silva
P
P
Schalling
M
M
Bertorello
A M
AM
D000818:Animals; D000855:Anorexia / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D001142:Aromatic-L-Amino-Acid Decarboxylases / Q000378:metabolism; D015306:Biogenic Monoamines / Q000378:metabolism; D003342:Corpus Striatum / Q000378:metabolism* / Q000473:pathology; D004298:Dopamine / Q000378:metabolism* / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects / Q000235:genetics; D051379:Mice; D008818:Mice, Neurologic Mutants; D009474:Neurons / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D010641:Phenotype; D000254:Sodium-Potassium-Exchanging ATPase / Q000235:genetics / Q000378:metabolism; D009435:Synaptic Transmission / Q000502:physiology; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
D015306:Biogenic Monoamines; D014446:Tyrosine 3-Monooxygenase; D001142:Aromatic-L-Amino-Acid Decarboxylases; D000254:Sodium-Potassium-Exchanging ATPase; D004298:Dopamine