title: Fate of Legionella pneumophila in macrophages of C57BL/6 chronic granulomatous disease mice.
abstract: We compared the intracellular survival and growth of Legionella pneumophila Philadelphia-1 in peritoneal macrophages obtained from A/J, C57BL/6, and X-linked chronic granulomatous disease (CGD) mice produced from C57BL/6 strain. The initial killing was observed in A/J and C57BL/6 macrophages at 2, 4 and 6 hr after in vitro phagocytosis, but not in the CGD macrophages. Thereafter, there was a 10-fold increase of CFU in A/J macrophages. The bacteria, however, did not proliferate in C57BL/6 and CGD macrophages at 24 or 48 hr after in vitro phagocytosis. These results suggest that effector molecules for the initial killing are a superoxide anion and its metabolites, and Lgn1 gene product inhibits the intracellular growth of L. pneumophila independently of NADPH oxidase.
authors.affiliation: Department of Bacteriology, Faculty of Medical Sciences, Kyushu University, Fukuoka, Fukuoka, Japan. msaito@bact.med.kyushu-u.ac.jp
authors.forename: M
authors.initials: M
authors.lastname: Saito
authors.forename: H
authors.initials: H
authors.lastname: Kajiwara
authors.forename: H
authors.initials: H
authors.lastname: Miyamoto
authors.forename: S
authors.initials: S
authors.lastname: Yoshida
mesh_terms: D000818:Animals; D005260:Female; D006105:Granulomatous Disease, Chronic / Q000276:immunology / Q000382:microbiology*; D016952:Legionella pneumophila / Q000254:growth & development* / Q000472:pathogenicity; D007877:Legionnaires' Disease / Q000276:immunology / Q000382:microbiology*; D017737:Macrophages, Peritoneal / Q000382:microbiology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010587:Phagocytosis / Q000276:immunology