title: Linkage to atopy on chromosome 19 in north-eastern Italian families with allergic asthma.
abstract: BACKGROUND
  Allergic asthma is a multifactorial disease for which there is a widely assessed, although poorly understood, genetic involvement. Genome-wide screens reported evidence for linkage of allergic asthma-related phenotypes to several chromosomal locations. Markers on chromosome 19 have been linked to allergic asthma phenotypes in different populations in independent studies.
  
  
  OBJECTIVE
  The aim of this study was to perform a genetic linkage analysis on chromosome 19 to search for DNA markers linked to phenotypes related to allergic asthma.
  
  
  METHODS
  Using non-parametric multipoint linkage analysis on a total of 22 random DNA markers in 2 stages, a sample of 111 families (542 subjects) from north-eastern Italy, recruited through an asthmatic allergic proband, was investigated. Phenotypes examined were: clinical asthma, total serum elevated IgE, skin prick test positivity, bronchial hyper-responsiveness, and atopy defined as skin prick test positivity and/or elevated IgE. Simulation studies were performed to confirm the significance of the results.
  
  
  RESULTS
  A novel linkage of atopy and skin prick test positivity to marker D19S601 (19q13.3) was found. Modest evidence for linkage of atopy, skin prick test positivity, and IgE was also found to marker D19S591 (19p13.3). Simulation analysis for atopy gave an NPL-Z > 3.326 in 2 replicates out of 1000 (P = 0.002) for D19S601, and an NPL-Z > 2.56 in 16 replicates out of 1000 (P = 0.016) for D19S591.
  
  
  CONCLUSIONS
  On chromosome 19, suggestive linkage of atopy and skin prick test positivity with marker D19S601 (19q13.3) and modest evidence of linkage of marker D19S591 (19p13.3) to the atopic phenotypes investigated were found. These results suggest that these regions may contain susceptibility loci associated to atopic phenotypes.
authors.affiliation: Department of Mother and Child, Biology and Genetics (DMIBG), University of Verona, Verona, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Venanzi
authors.forename: G
authors.initials: G
authors.lastname: Malerba
authors.forename: R
authors.initials: R
authors.lastname: Galavotti
authors.forename: M C
authors.initials: MC
authors.lastname: Lauciello
authors.forename: E
authors.initials: E
authors.lastname: Trabetti
authors.forename: G
authors.initials: G
authors.lastname: Zanoni
authors.forename: L
authors.initials: L
authors.lastname: Pescollderungg
authors.forename: L C
authors.initials: LC
authors.lastname: Martinati
authors.forename: A L
authors.initials: AL
authors.lastname: Boner
authors.forename: P F
authors.initials: PF
authors.lastname: Pignatti
mesh_terms: D000328:Adult; D001249:Asthma / Q000453:epidemiology / Q000235:genetics*; D002648:Child; D002874:Chromosome Mapping; D002888:Chromosomes, Human, Pair 19 / Q000235:genetics*; D008040:Genetic Linkage / Q000235:genetics*; D005819:Genetic Markers / Q000235:genetics; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000453:epidemiology / Q000235:genetics*; D007558:Italy / Q000453:epidemiology; D010641:Phenotype
chemical_list: D005819:Genetic Markers