[The inhibitory effect of oxymatrine on hepatitis C virus in vitro].
OBJECTIVE
To study the inhibitory effect of oxymatrine on HCV in vitro.


METHODS
SMMC-7721 cells transfected with pBK-HCV using lipofectin transfection protocal were treated with oxymatrine. bDNA signal amplification assay and MTT colorimetric assay were used for intracellular HCV RNA and cytotoxicity.


RESULTS
The stable HCV expression cell model established here could be used as a tool for medicine screen. Oxymatrine at the concentration of 100~1000 mug/ml could significantly decrease the level of intracellular HCV RNA. No cytotoxicity was shown in such concentrations.


CONCLUSIONS
Oxymatrine has an anti-HCV potential activity in vitro.
Chen
Y
Y
Shanghai Institute of Digestive Diseases, Renji Hospital, Shanghai Second Medical University, Shanghai 200001, China.
Li
J
J
Zeng
M
M
Lu
L
L
Qu
D
D
Mao
Y
Y
Fan
Z
Z
Hua
J
J
D000470:Alkaloids / Q000494:pharmacology*; D000998:Antiviral Agents / Q000494:pharmacology*; D016174:Hepacivirus / Q000187:drug effects*; D011807:Quinolizines; D000093842:Matrines
D000470:Alkaloids; D000998:Antiviral Agents; D011807:Quinolizines; C037573:oxymatrine; D000093842:Matrines