[Annexin V technique for the study of liver damage].
OBJECTIVE
To evaluate Annexin V technique for measuring hepatic apoptosis and to investigate the protective function of Rg1 and Rb1 on acute liver injury.


METHODS
LPS-treated acute liver injury and the protective effect of Rg1 and Rb1 were assessed by Annexin V double staining and PI staining measurements. The activity of sPLA(2) was measured by [(3)H]-labelled oleic acid method.


RESULTS
Annexin V showed a higher sensitivity and specificity than PI staining. It was only the Annexin V assay that could discriminate normal cells, apoptosis cells, and necrotic cells. Rg1 and Rb1 could reduce the percentage of hepatic apoptotic and necrotic cells (P<0.01) and activity of sPLA2 (P<0.01).


CONCLUSIONS
The Annexin V assay is an ideal method for measuring apoptosis presently. Rg1 and Rb1 have a definite protective effect on acute liver injury in rats.
Wu
F
F
Department of Laboratory, Chongqing Medical University, Chongqing 400016, China.
Kang
G
G
Jiang
J
J
D000818:Animals; D017304:Annexin A5 / Q000032:analysis*; D017209:Apoptosis; D008070:Lipopolysaccharides / Q000633:toxicity*; D008099:Liver / Q000187:drug effects* / Q000473:pathology; D010741:Phospholipases A / Q000378:metabolism; D011796:Quinacrine / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
D017304:Annexin A5; D008070:Lipopolysaccharides; D010741:Phospholipases A; D011796:Quinacrine