title: A prospective randomised concurrent comparison of the COBE Spectra Version 4.7, COBE Spectra Version 6 (auto PBSC) and Haemonetics MCS+ cell separators for leucapheresis in patients with haematological and non-haematological malignancies.
abstract: A prospective study of the CD34+ cell collection efficiency of three cell separators was undertaken comparing the mononuclear cell, CD34+ cell and CFU-GM yield. Twenty patients were entered in the study, all had received mobilising chemotherapy and daily G-CSF (5 microg/kg subcutaneously). The first leucapheresis was performed when the peripheral blood absolute CD34+ cell count was > or = 20 cells/microl. All patients underwent two leucaphereses on consecutive days. The patients were randomised to undergo either the first or second leucapheresis using the COBE Spectra Version 4.7 and then randomised to either the COBE Spectra Version 6 or Haemonetics MCS+ for the other leucapheresis. The target durations of the procedure on the COBE Spectra Version 4.7 and Version 6 were 180 min or two total blood volumes (TBV), and for the Haemonetics MCS+ was 20 cycles with four recirculations. All machines were operated on the 1997 software supplied by the respective manufacturers. The time taken for the procedure was significantly longer with both the Haemonetics MCS+ and the COBE Spectra Version 6 than the COBE Spectra Version 4.7. Both COBE Spectra versions processed significantly larger volumes of blood than the Haemonetics MCS+. The absolute yield of mononuclear cells, CFU-GM and CD34+ cells were all significantly lower with the Haemonetics MCS+ compared with both COBE Spectra Versions, as were the yields per unit volume of blood processed. There was no significant difference in the reduction in the platelet count following leucapheresis with any of the machines.
authors.affiliation: Clinical Apheresis Unit, Glasgow Royal Infirmary, Scotland, UK.
authors.forename: R H
authors.initials: RH
authors.lastname: Green
authors.forename: D
authors.initials: D
authors.lastname: Watson
authors.forename: S
authors.initials: S
authors.lastname: Buchanan
authors.forename: A E
authors.initials: AE
authors.lastname: Morrison
mesh_terms: D018952:Antigens, CD34; D001772:Blood Cell Count; D002469:Cell Separation / Q000295:instrumentation* / Q000592:standards; D005260:Female; D019337:Hematologic Neoplasms / Q000628:therapy*; D006801:Humans; D007937:Leukapheresis / Q000295:instrumentation* / Q000379:methods / Q000592:standards; D007963:Leukocytes, Mononuclear / Q000166:cytology; D008297:Male; D008875:Middle Aged; D023461:Myeloid Progenitor Cells / Q000166:cytology; D011446:Prospective Studies
chemical_list: D018952:Antigens, CD34