title: Folding and stability of sweet protein single-chain monellin. An insight to protein engineering.
abstract: Engineered single-chain monellin (SCM) proteins were constructed by recombinant technology without disrupting the topology and sweet activity of native protein. Data from 8-anilinonaphthalene-1-sulfonic acid fluorescence, size-exclusion chromatography, and heteronuclear NMR strongly suggest the presence of a folding intermediate at 1.5 m GdnHCl for SCM protein. The structural feature of the folding intermediate from NMR data reveals that the secondary structures became mostly unstable, and protein experiences a dynamic equilibrium between native and unfolded state. All backbone amide protons exchange within 10 min, which imply that no stable hydrogen bonds exist in the secondary structural regions in the folding intermediate. From equilibrium unfolding and mutagenesis studies, the unfolding transition midpoints of mutant proteins gradually shifted toward lower denaturant concentration, indicating stability reductions of mutant proteins. Our results suggest that stability and folding pathways of SCM proteins could be regulated by a combined study of spectroscopy and mutagenesis, and these studies will provide useful information for understanding the folding kinetics of novel engineered proteins.
authors.affiliation: Department of Biochemistry and Protein Network Research Center, College of Science, Yonsei University, Seoul 120-740, Science Town, Taejon 305-380, Korea.
authors.forename: Y H
authors.initials: YH
authors.lastname: Sung
authors.forename: H D
authors.initials: HD
authors.lastname: Hong
authors.forename: C
authors.initials: C
authors.lastname: Cheong
authors.forename: J H
authors.initials: JH
authors.lastname: Kim
authors.forename: J M
authors.initials: JM
authors.lastname: Cho
authors.forename: Y R
authors.initials: YR
authors.lastname: Kim
authors.forename: W
authors.initials: W
authors.lastname: Lee
mesh_terms: D000595:Amino Acid Sequence; D002850:Chromatography, Gel; D008958:Models, Molecular; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D010940:Plant Proteins / Q000737:chemistry*; D015202:Protein Engineering; D017510:Protein Folding
chemical_list: D010940:Plant Proteins; C002541:monellin protein, Dioscoreophyllum cumminsii