title: Synergy between Hoxa1 and Hoxb1: the relationship between arch patterning and the generation of cranial neural crest.
abstract: Hoxa1 and Hoxb1 have overlapping synergistic roles in patterning the hindbrain and cranial neural crest cells. The combination of an ectoderm-specific regulatory mutation in the Hoxb1 locus and the Hoxa1 mutant genetic background results in an ectoderm-specific double mutation, leaving the other germ layers impaired only in Hoxa1 function. This has allowed us to examine neural crest and arch patterning defects that originate exclusively from the neuroepithelium as a result of the simultaneous loss of Hoxa1 and Hoxb1 in this tissue. Using molecular and lineage analysis in this double mutant background we demonstrate that presumptive rhombomere 4, the major site of origin of the second pharyngeal arch neural crest, is reduced in size and has lost the ability to generate neural crest cells. Grafting experiments using wild-type cells in cultured normal or double mutant mouse embryos demonstrate that this is a cell-autonomous defect, suggesting that the formation or generation of cranial neural crest has been uncoupled from segmental identity in these mutants. Furthermore, we show that loss of the second arch neural crest population does not have any adverse consequences on early patterning of the second arch. Signalling molecules are expressed correctly and pharyngeal pouch and epibranchial placode formation are unaffected. There are no signs of excessive cell death or loss of proliferation in the epithelium of the second arch, suggesting that the neural crest cells are not the source of any indispensable mitogenic or survival signals. These results illustrate that Hox genes are not only necessary for proper axial specification of the neural crest but that they also play a vital role in the generation of this population itself. Furthermore, they demonstrate that early patterning of the separate components of the pharyngeal arches can proceed independently of neural crest cell migration.
authors.affiliation: Division of Developmental Neurobiology, MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK.
authors.forename: A
authors.initials: A
authors.lastname: Gavalas
authors.forename: P
authors.initials: P
authors.lastname: Trainor
authors.forename: L
authors.initials: L
authors.lastname: Ariza-McNaughton
authors.forename: R
authors.initials: R
authors.lastname: Krumlauf
mesh_terms: D000818:Animals; D019521:Body Patterning; D001934:Branchial Region / Q000196:embryology*; D019070:Cell Lineage; D002465:Cell Movement; D002470:Cell Survival; D046508:Culture Techniques; D018398:Homeodomain Proteins / Q000235:genetics / Q000502:physiology*; D051379:Mice; D009154:Mutation; D009432:Neural Crest / Q000166:cytology / Q000196:embryology*; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology*; D000099261:Homeobox A1 Protein
chemical_list: D018398:Homeodomain Proteins; D014157:Transcription Factors; D000099261:Homeobox A1 Protein; C101121:HOXB1 homeodomain protein