title: Changes in fatty acid composition of Pseudomonas aeruginosa ATCC 15442 induced by growth conditions: consequences of resistance to quaternary ammonium compounds.
abstract: The effects of changes in the fatty acid composition of Pseudomonas aeruginosa induced by growth conditions on its resistance to two quaternary ammonium compounds (QAC) were investigated. The temperature and growth phase were the most influential parameters affecting the fatty acid composition of this bacterium. Furthermore, the formation of saturated fatty acids and cyclopropane fatty acids was stimulated by increasing the temperature, whereas the proportion of unsaturated fatty acids fell. The degree of saturation and the proportion of cyclopropane fatty acids increased in the course of the exponential and stationary phases. These modifications mostly concerned the inner membrane of the bacterium. Resistance of P. aeruginosa to both QAC tested was not significantly influenced by temperature and growth phase variations. Thus, resistance to the two QAC did not seem to be dependent on modifications of the fatty acid composition of the inner membrane.
authors.affiliation: Ecole Nationale Supérieure des Industries Agricoles et Alimentaires, Département de Microbiologie Industrielle, Massy, France.
authors.forename: F
authors.initials: F
authors.lastname: Dubois-Brissonnet
authors.forename: C
authors.initials: C
authors.lastname: Malgrange
authors.forename: L
authors.initials: L
authors.lastname: Guérin-Méchin
authors.forename: B
authors.initials: B
authors.lastname: Heyd
authors.forename: J Y
authors.initials: JY
authors.lastname: Leveau
mesh_terms: D001548:Benzalkonium Compounds / Q000494:pharmacology; D003470:Culture Media; D024881:Drug Resistance, Bacterial; D005227:Fatty Acids / Q000032:analysis*; D008826:Microbial Sensitivity Tests / Q000379:methods; D011550:Pseudomonas aeruginosa / Q000737:chemistry / Q000187:drug effects* / Q000254:growth & development; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology*; D013696:Temperature
chemical_list: D001548:Benzalkonium Compounds; D003470:Culture Media; D005227:Fatty Acids; D000644:Quaternary Ammonium Compounds; C005883:zephiramine; C027118:didecyldimethylammonium