title: Bioconcentration of nonylphenol in fathead minnows (Pimephales promelas).
abstract: Bioconcentration of p-nonylphenol (NP) by fathead minnows was determined under laboratory conditions. Fish were exposed continuously for 42 days to 0.33, 0.93 and 2.36 microg NP/l in a flow-through system. NP was Soxhlet extracted from whole fish homogenates with dichloromethane (DCM). The resulting extract was concentrated and bulk lipids removed by gel permeation and silica-gel chromatography. Compounds were identified and quantified by reverse-phase high-pressure liquid chromatography (RP-HPLC) with fluorescence detection. Mass spectrometry was used for verification of peak assignments. Bioconcentration factors (BCFs) ranged from 245 to 380.
authors.affiliation: Department of Zoology, Institute of Environmental Toxicology, National Food Safety and Toxicology Center, Michigan State University, East Lansing 48824-1311, USA. shane.snyder@lvvwd.com
authors.forename: S A
authors.initials: SA
authors.lastname: Snyder
authors.forename: T L
authors.initials: TL
authors.lastname: Keith
authors.forename: S L
authors.initials: SL
authors.lastname: Pierens
authors.forename: E M
authors.initials: EM
authors.lastname: Snyder
authors.forename: J P
authors.initials: JP
authors.lastname: Giesy
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003530:Cyprinidae; D015180:Electrophoresis, Gel, Two-Dimensional; D008055:Lipids; D013058:Mass Spectrometry; D010636:Phenols / Q000493:pharmacokinetics*; D014018:Tissue Distribution; D014874:Water Pollutants, Chemical / Q000493:pharmacokinetics*
chemical_list: D008055:Lipids; D010636:Phenols; D014874:Water Pollutants, Chemical; C025256:nonylphenol