title: Effect of parasympathetic decentralization on interferon-gamma release from rat submandibular lymph nodes in vitro.
abstract: The aim of this study was to examine the regulation of interferon (IFN)-gamma release by cells from submandibular lymph nodes of rats subjected to a unilateral parasympathetic decentralization by severing the ipsilateral chorda tympani 7 days earlier. Cells obtained from contralateral sham-operated submandibular lymph nodes were employed as control. Parasympathetic decentralization of lymph nodes resulted in significantly less IFN-gamma release as compared to that found in innervated lymph nodes. Mitogens (lipopolysaccharide, concanavalin A) stimulated IFN-gamma release in cells derived from the innervated lymph nodes only. The muscarinic agonist metacholine decreased IFN-gamma release in cells derived from innervated lymph nodes. At the highest concentration employed (10(-4) M), metacholine suppressed the stimulatory effect of mitogens on IFN-gamma release in cells of innervated lymph nodes while the muscarinic antagonist atropine (10(-8) - 10(-4) M) lacked to affect IFN-gamma release. Addition of nicotine (10(-5) - 10(-3) M) failed to modify IFN-gamma release. The results support the occurrence of significant effects of local parasympathetics in modulating IFN-gamma release by submandibular lymph nodes.
authors.affiliation: Departamento de Bioquímica y Biología Molecular III, Facultad de Medicina, Universidad Complutense, Madrid, Spain
authors.forename: A I
authors.initials: AI
authors.lastname: Esquifino
authors.forename: P O
authors.initials: PO
authors.lastname: Castrillón
authors.forename: A
authors.initials: A
authors.lastname: Arce
authors.forename: R A
authors.initials: RA
authors.lastname: Cutrera
authors.forename: D P
authors.initials: DP
authors.lastname: Cardinali
mesh_terms: D000818:Animals; D001285:Atropine / Q000494:pharmacology; D001339:Autonomic Fibers, Preganglionic / Q000502:physiology; D002814:Chorda Tympani Nerve / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D003208:Concanavalin A / Q000494:pharmacology; D066298:In Vitro Techniques; D007371:Interferon-gamma / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology; D018727:Muscarinic Antagonists / Q000494:pharmacology; D009538:Nicotine / Q000494:pharmacology; D018722:Nicotinic Agonists / Q000494:pharmacology; D010275:Parasympathetic Nervous System / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D011976:Receptors, Muscarinic / Q000502:physiology; D013363:Submandibular Gland / Q000294:innervation* / Q000378:metabolism*
chemical_list: D008070:Lipopolysaccharides; D018727:Muscarinic Antagonists; D018722:Nicotinic Agonists; D011976:Receptors, Muscarinic; D003208:Concanavalin A; D009538:Nicotine; D001285:Atropine; D007371:Interferon-gamma