Complete nucleotide sequence of avian paramyxovirus type 6 isolated from ducks.
There are nine serotypes of avian paramyxovirus (APMV). Only the genome of APMV type 1 (APMV-1), also called Newcastle disease virus (NDV), has been completely sequenced. In this study, the complete nucleotide sequence of an APMV-6 serotype isolated from ducks is reported. The 16236 nt genome encodes eight proteins, nucleocapsid protein (NP), phosphoprotein (P), V protein, matrix protein (M), fusion protein (F), small hydrophobic (SH) protein, haemagglutinin-neuraminidase (HN) protein and large (L) protein, which are flanked by a 55 nt leader sequence and a 54 nt trailer sequence. Sequence comparison reveals that the protein sequences of APMV-6 are most closely related to those of APMV-1 (NDV) and -2, with sequence identities ranging from 22 to 44%. However, APMV-6 contains a gene that might encode the SH protein, which is absent in APMV-1, but present in the rubulaviruses simian virus type 5 and mumps virus. The presence of an SH gene in APMV-6 might provide a link between the evolution of APMV and rubulaviruses. Phylogenetic analysis demonstrates that APMV-6, -1, -2 (only the F and HN sequences were available for analysis) and -4 (only the HN sequences were available for analysis) all cluster into a single lineage that is distinct from other paramyxoviruses. This result suggests that APMV should constitute a new genus within the subfamily Paramyxovirinae.
Chang
P-C
PC
Graduate Institute of Veterinary Microbiology1 and Department of Veterinary Medicine2, National Chung Hsing University, Taichung, Taiwan, Republic of China.
Hsieh
M-L
ML
Graduate Institute of Veterinary Microbiology1 and Department of Veterinary Medicine2, National Chung Hsing University, Taichung, Taiwan, Republic of China.
Shien
J-H
JH
Graduate Institute of Veterinary Microbiology1 and Department of Veterinary Medicine2, National Chung Hsing University, Taichung, Taiwan, Republic of China.
Graham
D A
DA
Veterinary Science Division, Department of Agriculture and Rural Development for Northern Ireland, Stoney Road, Stormont, Belfast BT4 3SD, UK2.
Lee
M-S
MS
Graduate Institute of Veterinary Microbiology1 and Department of Veterinary Medicine2, National Chung Hsing University, Taichung, Taiwan, Republic of China.
Shieh
H K
HK
Graduate Institute of Veterinary Microbiology1 and Department of Veterinary Medicine2, National Chung Hsing University, Taichung, Taiwan, Republic of China.
D000818:Animals; D018109:Avulavirus / Q000145:classification* / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000737:chemistry; D004372:Ducks / Q000821:virology*; D016679:Genome, Viral; D008969:Molecular Sequence Data; D010802:Phylogeny; D014760:Viral Fusion Proteins / Q000737:chemistry
D004279:DNA, Viral; D014760:Viral Fusion Proteins