[Improvement of proliferation of intrasplenically transplanted fetal hepatocytes after immunoadsorption].
OBJECTIVE
To investigate whether removing the compartments of hemopoietic stem cells by immunoadsorption would improve the proliferation of fetal hepatocytes after transplantation.


METHODS
The purification of fetal hepatocytes was performed by immunoadsorption, and the proliferative capability of fetal hepatocytes after intrasplenic transplantation was evaluated by image analysis and flowcytometry analysis.


RESULTS
The yield of fetal hepatocytes after immnoadsorption was (2~3)+/-10(7) in total per fetal liver and the viability is over 85%. The square densities of fetal hepatocytes with image analysis at 3, 7, and 14 days after transplantation were (1603+/-337), (3788+/-605), (8129+/-1025) micrometer(2)/mm(2), and the adult hepatocytes were (1007+/-128), (2013+/-242), (3780+/- 772) mum(2)/mm(2) accordingly. The S phase rates of fetal hepatocytes by flowcytometry analysis were (14.25+/-4.11)%, (16.07+/-5.35)%, and (17.32+/-5.17)%, and the rates of adult hepatocytes were (5.66+/-1.02)%, (4.98+/- 0.74)%, and (6.83+/-1.37)% correspondingly. The S phase rates and the square densities of fetal hepatocytes were significantly higher than those of adult hepatocytes.


CONCLUSIONS
Fetal hepatocytes are highly proliferative and potentially suitable as donor cells in hepatocytes transplantation after immunoadsorption.
Xu
G
G
Department of General Surgery and Lin Baixin Medical Research Center, Sun Yat-sen Memorial Hospital of Sun Yat-sen University of Medical Sciences, Guangzhou 510120, China.
Zhou
X
X
Weu
J
J
Min
J
J
Chen
J
J
D000818:Animals; D002455:Cell Division; D005333:Fetus; D022781:Hepatocytes / Q000502:physiology / Q000637:transplantation*; D007163:Immunosorbent Techniques; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013154:Spleen