Identification and quantification of polychlorinated biphenyls and some endocrine disrupting pesticides in human adipose tissue from Finland.
Organochlorine pesticides and polychlorinated biphenyls (PCBs) were analysed in extracts of human adipose tissue. The samples consisted of abdominal, mammary, and perirenal fat tissue of 27 Finnish adult males and females. Lipids were separated from the low-molecular compounds by preparative gel permeation chromatography (GPC) and the extracts were further cleaned-up using Florisil chromatography. The compounds were analysed in the extracts by gas chromatography (GC) using electron capture detection and by GC-mass spectrometry in the selected ion monitoring mode. Of the 23 analysed pesticide residues only seven could be detected in the extracts. All the extracts contained the DDT metabolite 4,4'-DDE, hexachlorobenzene, and PCBs. Other compounds found in the extracts were 4,4'-DDT, 4,4'-DDD, pentachlorobenzene, and beta- and gamma-hexachlorocyclohexane (HCH). The antiandrogenic 4,4'-DDE was the most abundant pesticide with concentration levels ranging from 3.5 to 3229 ng/g lipids (ppb). The mean concentration of DDE was 567 ppb, of pesticides in all 1008 ppb and of PCBs 504 ppb. Several of the identified compounds have been shown to exhibit endocrine disrupting effects. Statistical analysis showed a positive age correlation of DDE in females and hexachlorobenzene in males. No statistically significant differences were found for concentrations of individual compounds between sexes.
Smeds
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Department of Organic Chemistry, Abo Akademi University, Turku, Finland. ansmeds@abo.fi
Saukko
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D000273:Adipose Tissue / Q000737:chemistry*; D000328:Adult; D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D002849:Chromatography, Gas; D004703:Endocrine System / Q000187:drug effects; D005260:Female; D005387:Finland; D006801:Humans; D007306:Insecticides / Q000032:analysis / Q000493:pharmacokinetics*; D008297:Male; D008875:Middle Aged; D011078:Polychlorinated Biphenyls / Q000032:analysis / Q000493:pharmacokinetics*; D012737:Sex Factors; D014018:Tissue Distribution
D007306:Insecticides; D011078:Polychlorinated Biphenyls