title: Drosophila neuroblasts sequentially express transcription factors which specify the temporal identity of their neuronal progeny.
abstract: Neural precursors often generate distinct cell types in a specific order, but the intrinsic or extrinsic cues regulating the timing of cell fate specification are poorly understood. Here we show that Drosophila neural precursors (neuroblasts) sequentially express the transcription factors Hunchback --> Krüppel --> Pdm --> Castor, with differentiated progeny maintaining the transcription factor profile present at their birth. Hunchback is necessary and sufficient for first-born cell fates, whereas Krüppel is necessary and sufficient for second-born cell fates; this is observed in multiple lineages and is independent of the cell type involved. We propose that Hunchback and Krüppel control early-born temporal identity in neuroblast cell lineages.
authors.affiliation: Institute of Neuroscience/Institute of Molecular Biology, Howard Hughes Medical Institute, 1254 University of Oregon, Eugene, OR 97403, USA.
authors.forename: T
authors.initials: T
authors.lastname: Isshiki
authors.forename: B
authors.initials: B
authors.lastname: Pearson
authors.forename: S
authors.initials: S
authors.lastname: Holbrook
authors.forename: C Q
authors.initials: CQ
authors.lastname: Doe
mesh_terms: D000818:Animals; D019070:Cell Lineage / Q000235:genetics / Q000502:physiology*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000235:genetics / Q000254:growth & development* / Q000502:physiology; D005786:Gene Expression Regulation; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism; D007605:Juvenile Hormones / Q000235:genetics / Q000378:metabolism; D051741:Kruppel-Like Transcription Factors; D009024:Morphogenesis / Q000235:genetics / Q000502:physiology; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D050809:POU Domain Factors; D012097:Repressor Proteins; D013997:Time Factors; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D018398:Homeodomain Proteins; D019476:Insect Proteins; D007605:Juvenile Hormones; C497244:Kr protein, Drosophila; D051741:Kruppel-Like Transcription Factors; D050809:POU Domain Factors; D012097:Repressor Proteins; D014157:Transcription Factors; C110593:cas protein, Drosophila; C061245:hb protein, Drosophila; C104121:nub protein, Drosophila