Effect of lectin from the ascidian on the growth and the adhesion of HeLa cells.
Cell adhesion molecules, some of which are lectins, play a key role in the control of normal and pathological processes of various living organisms. We found herein that N-acetyl-D-glucosamine-specific lectin, isolated from the ascidian Didemnum ternatanum (DTL), alters the growth properties of HeLa tumor cells depending on the anchorage. DTL was shown to increase the proliferation of HeLa cells grown in soft agar greatly (in anchorage-independent fashion). In contrast, DTL inhibits the proliferative activity of HeLa cells grown on solid substrate and acts as inductor of differentiation, slowing cell growth, increasing the cell attachment and spreading. Scanning electron microscopic data have demonstrated that DTL treatment resulted in pronounced changes of the shape and surface of HeLa cells. Changes of cellular morphology correlated with essential redistribution of actin microfilaments.
Odintsova
N A
NA
Laboratory of Cell Biophysics, Institute of Marine Biology, Far East Branch of RAS, Vladivostok, Russia.
Belogortseva
N I
NI
Khomenko
A V
AV
Chikalovets
I V
IV
Luk'yanov
P A
PA
D008841:Actin Cytoskeleton / Q000187:drug effects / Q000648:ultrastructure; D000199:Actins / Q000378:metabolism; D000362:Agar / Q000737:chemistry; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects*; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D048429:Cell Size / Q000187:drug effects; D002999:Clone Cells; D003094:Collagen / Q000378:metabolism; D003599:Cytoskeleton / Q000187:drug effects / Q000648:ultrastructure; D004305:Dose-Response Relationship, Drug; D006367:HeLa Cells; D006801:Humans; D037102:Lectins / Q000378:metabolism / Q000494:pharmacology*; D008855:Microscopy, Electron, Scanning; D014561:Urochordata
D000199:Actins; D037102:Lectins; D000362:Agar; D003094:Collagen