Changes in microtubules and microfilaments due to a combined effect of ultrasound and cytostatics in HeLa cells.
Treatment of HeLa cells with low intensity ultrasound and two cytostatic drugs, cycloplatin and methotrexate, resulted in a partial disassembly of microtubules and microfilaments. This disassembly was due to depolymerization and subsequent erroneous repolymeration of essential cytoskeletal proteins, resulting in formation of unusual arrangements, mainly small, granule-like structures. The combined action of ultrasound and cytostatics had a synergistic effect dependent on both the concentration of the drug and the time of sonication. The demonstrated changes in the cytoskeleton are considered to be non-specific to ultrasound treatment, reflecting only an altered vital state of the treated cells.
Skorpíková
J
J
Department of Biophysics, Faculty of Medicine, Masaryk University, Brno, Czech Republic. jskorpik@med.mini.cz
Dolníková
M
M
Hrazdira
I
I
Janisch
R
R
D008841:Actin Cytoskeleton / Q000187:drug effects* / Q000648:ultrastructure; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001704:Biopolymers; D006367:HeLa Cells / Q000187:drug effects / Q000648:ultrastructure*; D006801:Humans; D008727:Methotrexate / Q000494:pharmacology* / Q000633:toxicity; D008870:Microtubules / Q000187:drug effects* / Q000648:ultrastructure; D009944:Organoplatinum Compounds / Q000494:pharmacology* / Q000633:toxicity; D014465:Ultrasonics / Q000009:adverse effects
D000970:Antineoplastic Agents; D001704:Biopolymers; D009944:Organoplatinum Compounds; C055928:cycloplatam; D008727:Methotrexate