Reactivity of MEST-1 (antigalactofuranose) with Trypanosoma cruzi glycosylinositol phosphorylceramides (GIPCs): immunolocalization of GIPCs in acidic vesicles of epimastigotes.
Using confocal microscopy, MEST-1-positive immunofluorescence was observed within various Trypanosoma cruzi forms, except in cell-derived trypomastigotes. Glycosylinositol phosphorylceramides were identified by thin-layer chromatography immunostaining as the antigens recognized by MEST-1 in these parasites. In epimastigotes, labeling of MEST-1 coincided with acidic vesicles, indicating an internal localization of these glycoconjugates.
Suzuki
E
E
Departamento de Bioquímica, Universidade Federal de São Paulo, Escola Paulista de Medicina, Rua Botucatu 862, São Paulo, SP 04023-900, Brazil.
Mortara
R A
RA
Takahashi
H K
HK
Straus
A H
AH
D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism*; D000913:Antibodies, Protozoan / Q000378:metabolism; D000953:Antigens, Protozoan / Q000276:immunology / Q000378:metabolism; D005663:Furans / Q000276:immunology*; D005690:Galactose / Q000276:immunology*; D006028:Glycosphingolipids / Q000276:immunology* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D051379:Mice; D022161:Transport Vesicles / Q000276:immunology* / Q000378:metabolism; D014349:Trypanosoma cruzi / Q000254:growth & development* / Q000276:immunology* / Q000378:metabolism
D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D005663:Furans; D006028:Glycosphingolipids; C025524:inositolphosphoceramides; D005690:Galactose