title: Elevating dietary salt exacerbates hyperpnea-induced airway obstruction in guinea pigs.
abstract: Previous studies have indicated that increased dietary salt consumption worsens postexercise pulmonary function in humans with exercise-induced asthma (EIA). It has been suggested that EIA and hyperpnea-induced airway obstruction (HIAO) in guinea pigs (an animal model of EIA) are mediated by similar mechanisms. Therefore, the purpose of this study was to determine whether altering dietary salt consumption also exacerbated HIAO in guinea pigs. Furthermore, the potential pathway of action of dietary salt was investigated by blocking leukotriene (LT) production during HIAO in guinea pigs. Thirty-two male Hartley strain guinea pigs were split into two groups. One group (n = 16) of animals ingested a normal-salt diet (NSD) for 2 wk; the other group (n = 16) ingested a high-salt diet (HSD) for 2 wk. Thereafter, animals were anesthetized, cannulated, tracheotomized, and mechanically ventilated during a baseline period and during two dry gas hyperpnea challenges. After the first challenge, the animals were administered either saline or nordihydroguaiaretic acid, a LT inhibitor. Bladder urine was analyzed for electrolyte concentrations and urinary LTE(4). The HSD elicited higher airway inspiratory pressures (Ptr) than the NSD (P < 0.001) postchallenge. However, after infusion of the LT inhibitor and a second hyperpnea challenge, HIAO was blocked in both diet groups (P < 0.001). Nonetheless, the HSD group continued to demonstrate slightly higher Ptr than the NSD group (P < 0.05). Urinary LTE(4) excretion significantly increased in the HSD group compared with the NSD group within treatment groups. This study has demonstrated that dietary salt loading exacerbated the development of HIAO in guinea pigs and that LT release was involved in HIAO and may be moderated by changes in dietary salt loading.
authors.affiliation: School of Sport Science, Physical Education and Recreation, University of Wales Institute Cardiff, Wales CF23 6XD, United Kingdom. tmickleborough@uwic.ac.uk
authors.forename: T D
authors.initials: TD
authors.lastname: Mickleborough
authors.forename: R W
authors.initials: RW
authors.lastname: Gotshall
authors.forename: J
authors.initials: J
authors.lastname: Rhodes
authors.forename: A
authors.initials: A
authors.lastname: Tucker
authors.forename: L
authors.initials: L
authors.lastname: Cordain
mesh_terms: D000402:Airway Obstruction / Q000503:physiopathology*; D000818:Animals; D001835:Body Weight; D016084:Bronchoconstriction / Q000187:drug effects / Q000502:physiology; D004435:Eating; D004573:Electrolytes / Q000652:urine; D006168:Guinea Pigs; D006985:Hyperventilation / Q000503:physiopathology*; D017999:Leukotriene E4 / Q000037:antagonists & inhibitors / Q000652:urine; D016859:Lipoxygenase Inhibitors / Q000494:pharmacology; D008297:Male; D009637:Masoprocol / Q000494:pharmacology; D005082:Physical Exertion / Q000502:physiology*; D012965:Sodium Chloride / Q000494:pharmacology; D017673:Sodium Chloride, Dietary / Q000494:pharmacology*
chemical_list: D004573:Electrolytes; D016859:Lipoxygenase Inhibitors; D017673:Sodium Chloride, Dietary; D012965:Sodium Chloride; D017999:Leukotriene E4; D009637:Masoprocol