Formaldehyde production by Tris buffer in peptide formulations at elevated temperature.
This technical note provides evidence for the degradation of Tris buffer in a peptide formulation stored at elevated temperature (70 degrees C). The buffer degrades to liberate formaldehyde, which is shown to react with the peptide tyrosine residue. Those involved in peptide/protein formulation should be aware of the possible instability in this common biological buffer.
Song
Y
Y
Department of Pharmaceutical Chemistry, The University of Kansas, Lawrence, KS 66047, USA.
Schowen
R L
RL
Borchardt
R T
RT
Topp
E M
EM
D005557:Formaldehyde / Q000138:chemical synthesis*; D006358:Hot Temperature; D010455:Peptides / Q000737:chemistry*; D014325:Tromethamine / Q000737:chemistry*
D010455:Peptides; D014325:Tromethamine; D005557:Formaldehyde