Expression of the erythrocyte-binding antigen 175 in sporozoites and in liver stages of Plasmodium falciparum.
Screening of a Plasmodium falciparum genomic expression library for antigens expressed at the pre-erythrocytic stages resulted in the isolation of a recombinant phage (DG249) whose insert corresponded to regions II and III of a 175-kDa erythrocyte-binding antigen (EBA-175). EBA-175 is a parasite ligand implicated in red blood cell invasion. Reverse-transcriptase polymerase chain reaction, indirect immunofluorescent antibody test, and Western blot analysis confirmed that EBA-175 is expressed not only in blood-stage parasites but also in infected hepatocytes and on the sporozoite surface. The presence of EBA-175 on pre-erythrocytic parasites enhances the vaccine potential of this antigen by adding another target to the immune responses elicited by immunization.
Grüner
A C
AC
Unité de Parasitologie Biomédicale, Institut Pasteur, Paris, France.
Brahimi
K
K
Letourneur
F
F
Rénia
L
L
Eling
W
W
Snounou
G
G
Druilhe
P
P
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood / Q000276:immunology; D000953:Antigens, Protozoan / Q000235:genetics* / Q000302:isolation & purification; D015153:Blotting, Western; D002352:Carrier Proteins / Q000235:genetics* / Q000302:isolation & purification; D019084:Fluorescent Antibody Technique, Indirect; D015698:Genomic Library; D006801:Humans; D008099:Liver / Q000469:parasitology*; D016778:Malaria, Falciparum / Q000097:blood / Q000469:parasitology; D002679:Pan troglodytes / Q000469:parasitology; D010963:Plasmodium falciparum / Q000254:growth & development / Q000276:immunology*; D015800:Protozoan Proteins / Q000235:genetics* / Q000302:isolation & purification; D012333:RNA, Messenger / Q000032:analysis; D016053:RNA, Protozoan / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D002352:Carrier Proteins; D015800:Protozoan Proteins; D012333:RNA, Messenger; D016053:RNA, Protozoan; C065668:erythrocyte-binding antigen 175, Plasmodium