Rapid HPLC screening of jasmonate-induced increases in tobacco alkaloids, phenolics, and diterpene glycosides in Nicotiana attenuata.
A rapid, HPLC-based screening procedure for the main classes of secondary metabolites in Nicotiana attenuata leaves (alkaloids, phenolics, and diterpene glycosides) is reported. In a single step, leaves are extracted in aqueous acidified (0.5% acetic acid) methanol, and the extracted compounds are separated by reversed-phase HPLC with an acidic water/acetonitrile gradient in <30 min. The utility of the method in quantifying changes in the secondary metabolites after methyl jasmonate treatment of the plants, a treatment known to elicit resistance to herbivores in nature, is illustrated. Methyl jasmonate treatment elicited dramatic increases in some secondary metabolites (caffeoylputrescine, nicotine, and diterpene glycosides increased 12.5-, 1.4-, and 1.9-fold, respectively) but left others, such as rutin, unchanged. Such broad-based analytical screens will help characterize environmental and genetic changes in secondary metabolite profiles.
Keinänen
M
M
Department of Molecular Ecology, Max-Planck Institute for Chemical Ecology, Carl-Zeiss-Promenade 10, D-07745 Jena, Germany.
Oldham
N J
NJ
Baldwin
I T
IT
D000470:Alkaloids / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003517:Cyclopentanes / Q000494:pharmacology; D004224:Diterpenes / Q000378:metabolism; D054883:Oxylipins; D010636:Phenols / Q000378:metabolism; D018515:Plant Leaves / Q000737:chemistry; D010947:Plants, Toxic; D014026:Nicotiana / Q000737:chemistry* / Q000378:metabolism*
D000470:Alkaloids; D003517:Cyclopentanes; D004224:Diterpenes; D054883:Oxylipins; D010636:Phenols; C011006:jasmonic acid