In vitro effects of anti-Trypanosoma cruzi compounds on HSP60 levels.
The effect of nifurtimox and 5-nitro-2-thienylmalononitrile (5NO2TM), a novel compound with anti-Trypanosoma cruzi activity, upon vitality and HSP60 immunoreactivity of epimastigotes, has been determined. Both products showed no activity against epimastigotes at 0.1 microg/ml, while at 0.5 and 1 microg/ml, after 24 h of incubation, densities of these groups were significantly reduced, when compared to controls. An enhancement of HSP60 immunoreactivity was observed after 24 h in groups treated with 0.5 and 1 microg/ml nifurtimox. On the other hand, 5NO2TM had no effect.
Muelas-Serrano
S
S
Departamento de Parasitología, Facultad de Farmacia, Universidad Complutense de Madrid, Ciudad Universitaria, Spain.
Pérez-Serrano
J
J
Gómez-Barrio
A
A
Ochoa
C
C
Martínez
J
J
Rodríguez-Caabeiro
F
F
D000818:Animals; D014355:Chagas Disease / Q000469:parasitology; D018834:Chaperonin 60 / Q000187:drug effects / Q000378:metabolism*; D009547:Nifurtimox / Q000494:pharmacology*; D009574:Nitro Compounds / Q000494:pharmacology*; D021261:Parasitic Sensitivity Tests / Q000379:methods; D014344:Trypanocidal Agents / Q000494:pharmacology*; D014349:Trypanosoma cruzi / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism
D018834:Chaperonin 60; D009574:Nitro Compounds; D014344:Trypanocidal Agents; D009547:Nifurtimox