Hydrolysis of plasmid DNA catalyzed by Co(III) complex of cyclen attached to polystyrene.
Reactivity of the Co(III) complex of cyclen (CoCyc) in the hydrolytic cleavage of supercoiled pUC18 DNA leading to the formation of the corresponding open circular form was enhanced by >200 times upon attachment of CoCyc to cross-linked polystyrenes. Thus, half-lives as short as 40 min were achieved by the resin-based CoCyc in cleavage of the supercoiled DNA at 4 degrees C.
Jeung
C S
CS
Artzyme Biotech Corporation, 403-1, Silim-2-Dong, Seoul 151-012, South Korea.
Kim
C H
CH
Min
K
K
Suh
S W
SW
Suh
J
J
D003035:Cobalt / Q000737:chemistry*; D000080027:Cyclams; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D006571:Heterocyclic Compounds / Q000737:chemistry*; D006868:Hydrolysis; D007700:Kinetics; D009942:Organometallic Compounds / Q000737:chemistry*; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism*; D011137:Polystyrenes / Q000737:chemistry*; D011145:Polyvinyls / Q000737:chemistry
D000080027:Cyclams; D006571:Heterocyclic Compounds; D009942:Organometallic Compounds; D011137:Polystyrenes; D011145:Polyvinyls; C442399:cyclencobalt(III); C442400:poly(chloromethylstyrene-co-divinylbenzene); D003035:Cobalt; D004247:DNA; C038072:cyclen