An alternative approach for enumeration of Escherichia coli in foods.
An assay to screen Escherichia coli in foods using MUG supplemented lauryl sulfate tryptose (LST) broth instead of tryptone water (TW) medium was evaluated. The results presented in this paper suggest that this method is more sensitive for lower levels of E. coli, faster (16-18 h vs. 6-10 days) and less expensive (2.454 vs. 2.887 EURO/sample) than the standard ISO procedure. Thus, this method may be beneficial for use when both fecal coliforms and E. coli analyses are required in food systems.
Cakir
I
I
Department of Food Engineering, Faculty of Agriculture, Ankara University, Diskapi, Turkey.
Dogan
H B
HB
Halkman
A K
AK
Worobo
R W
RW
D015169:Colony Count, Microbial / Q000191:economics / Q000379:methods*; D003365:Costs and Cost Analysis; D003470:Culture Media; D004755:Enterobacteriaceae / Q000254:growth & development* / Q000302:isolation & purification; D004926:Escherichia coli / Q000254:growth & development* / Q000302:isolation & purification; D005243:Feces / Q000382:microbiology; D005456:Fluorescent Dyes / Q000378:metabolism; D005516:Food Microbiology; D006923:Hymecromone / Q000031:analogs & derivatives / Q000378:metabolism; D012680:Sensitivity and Specificity; D013997:Time Factors
D003470:Culture Media; D005456:Fluorescent Dyes; D006923:Hymecromone; C034888:4-methylumbelliferyl glucuronide