title: Sequence typing confirms that Campylobacter jejuni strains associated with Guillain-Barré and Miller-Fisher syndromes are of diverse genetic lineage, serotype, and flagella type.
abstract: Guillain-Barré syndrome (GBS) and Miller-Fisher syndrome (MFS) are correlated with prior infection by Campylobacter jejuni in up to 40% of cases. Nucleotide sequence-based typing of 25 C. jejuni isolates associated with neuropathy permitted robust comparisons with equivalent data from approximately 800 C. jejuni isolates not associated with neuropathy. A total of 13 genetic lineages and 20 flaA short variable region nucleotide sequences were present among the 25 isolates. A minority of isolates (4 of 25) had the flaA short variable region nucleotide sequences that were previously proposed as a marker for GBS-associated isolates. These 4 isolates probably represented the Penner serotype 19 lineage, which has been proposed to have an association with GBS.
authors.affiliation: Wellcome Trust Centre for the Epidemiology of Infectious Disease, Department of Zoology, University of Oxford, Oxford, OX1 3FY, United Kingdom.
authors.forename: K E
authors.initials: KE
authors.lastname: Dingle
authors.forename: N
authors.initials: N
authors.lastname: Van Den Braak
authors.forename: F M
authors.initials: FM
authors.lastname: Colles
authors.forename: L J
authors.initials: LJ
authors.lastname: Price
authors.forename: D L
authors.initials: DL
authors.lastname: Woodward
authors.forename: F G
authors.initials: FG
authors.lastname: Rodgers
authors.forename: H P
authors.initials: HP
authors.lastname: Endtz
authors.forename: A
authors.initials: A
authors.lastname: Van Belkum
authors.forename: M C
authors.initials: MC
authors.lastname: Maiden
mesh_terms: D015373:Bacterial Typing Techniques; D001483:Base Sequence; D002169:Campylobacter Infections / Q000150:complications* / Q000382:microbiology; D016123:Campylobacter jejuni / Q000145:classification* / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D005407:Flagella / Q000145:classification* / Q000235:genetics; D005408:Flagellin / Q000235:genetics; D014644:Genetic Variation; D020275:Guillain-Barre Syndrome / Q000382:microbiology*; D006801:Humans; D019846:Miller Fisher Syndrome / Q000382:microbiology*; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D012703:Serotyping
chemical_list: D004269:DNA, Bacterial; D005408:Flagellin; C067138:flaA protein, bacteria