title: Histone deacetylase activity represses gamma interferon-inducible HLA-DR gene expression following the establishment of a DNase I-hypersensitive chromatin conformation.
abstract: Expression of the retinoblastoma tumor suppressor protein (Rb) is required for gamma interferon (IFN-gamma)-inducible major histocompatibility complex class II gene expression and transcriptionally productive HLA-DRA promoter occupancy in several human tumor cell lines. Treatment of these Rb-defective tumor cell lines with histone deacetylase (HDAC) inhibitors rescued IFN-gamma-inducible HLA-DRA and -DRB mRNA and cell surface protein expression, demonstrating repression of these genes by endogenous cellular HDAC activity. Additionally, Rb-defective, transcriptionally incompetent tumor cells retained the HLA-DRA promoter DNase I-hypersensitive site. Thus, HDAC-mediated repression of the HLA-DRA promoter occurs following the establishment of an apparent nucleosome-free promoter region and before transcriptionally productive occupancy of the promoter by the required transactivators. Repression of HLA-DRA promoter activation by HDAC activity likely involves a YY1 binding element located in the first exon of the HLA-DRA gene. Chromatin immunoprecipitation experiments localized YY1 to the HLA-DRA gene in Rb-defective tumor cells. Additionally, mutation of the YY1 binding site prevented repression of the promoter by HDAC1 and partially prevented activation of the promoter by trichostatin A. Mutation of the octamer element also significantly reduced the ability of HDAC1 to confer repression of inducible HLA-DRA promoter activation. Treatment of Rb-defective tumor cells with HDAC inhibitors greatly reduced the DNA binding activity of Oct-1, a repressor of inducible HLA-DRA promoter activation. These findings represent the first evidence that HDAC activity can repress IFN-gamma-inducible HLA class II gene expression and also demonstrate that HDAC activity can contribute to promoter repression following the establishment of a DNase I-hypersensitive chromatin conformation.
authors.affiliation: Department of Biochemistry and Molecular Biology, College of Medicine, H. Lee Moffitt Cancer Center and Research Institute, University of South Florida, Tampa, Florida 33612, USA.
authors.forename: A
authors.initials: A
authors.lastname: Osborne
authors.forename: H
authors.initials: H
authors.lastname: Zhang
authors.forename: W M
authors.initials: WM
authors.lastname: Yang
authors.forename: E
authors.initials: E
authors.lastname: Seto
authors.forename: G
authors.initials: G
authors.lastname: Blanck
mesh_terms: D002087:Butyrates / Q000494:pharmacology; D002843:Chromatin / Q000737:chemistry*; D004268:DNA-Binding Proteins / Q000378:metabolism / Q000502:physiology; D003850:Deoxyribonuclease I / Q000737:chemistry; D004791:Enzyme Inhibitors / Q000494:pharmacology; D050981:Erythroid-Specific DNA-Binding Factors; D006684:HLA-DR Antigens / Q000096:biosynthesis / Q000235:genetics*; D056572:Histone Deacetylase Inhibitors; D006655:Histone Deacetylases / Q000378:metabolism* / Q000502:physiology*; D051863:Host Cell Factor C1; D006801:Humans; D006877:Hydroxamic Acids / Q000494:pharmacology; D007371:Interferon-gamma / Q000494:pharmacology*; D058610:Isobutyrates; D009154:Mutation; D009690:Nucleic Acid Conformation; D050811:Octamer Transcription Factor-1; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000096:biosynthesis; D012097:Repressor Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000502:physiology; D016160:Retinoblastoma Protein / Q000235:genetics / Q000502:physiology; D014157:Transcription Factors / Q000378:metabolism / Q000502:physiology; D015533:Transcriptional Activation; D014407:Tumor Cells, Cultured; D050986:YY1 Transcription Factor
chemical_list: D002087:Butyrates; D002843:Chromatin; D004268:DNA-Binding Proteins; D004791:Enzyme Inhibitors; D050981:Erythroid-Specific DNA-Binding Factors; C497692:HCFC1 protein, human; D006684:HLA-DR Antigens; D056572:Histone Deacetylase Inhibitors; D051863:Host Cell Factor C1; D006877:Hydroxamic Acids; D058610:Isobutyrates; D050811:Octamer Transcription Factor-1; C494165:POU2F1 protein, human; D012333:RNA, Messenger; D012097:Repressor Proteins; D016160:Retinoblastoma Protein; D014157:Transcription Factors; D050986:YY1 Transcription Factor; C494698:YY1 protein, human; C012589:trichostatin A; D007371:Interferon-gamma; C020380:isobutyric acid; D003850:Deoxyribonuclease I; D006655:Histone Deacetylases