Localization of CYP86B1 in the outer envelope of chloroplasts.
CYP86B1 was cloned from a cDNA library and the protein expressed in E. coli. The protein gave the expected carbon monoxide difference spectrum. Using in vitro import assays with isolated pea chloroplasts, CYP86B1 was shown to be associated with the outer chloroplastic envelope membrane. This study provides the first direct evidence for a chloroplast-localized cytochrome P450-dependent monooxygenase.
Watson
C J
CJ
Department of Botany and Plant Pathology, Purdue University, W. Lafayette, IN 47907, USA.
Froehlich
J E
JE
Josefsson
C A
CA
Chapple
C
C
Durst
F
F
Benveniste
I
I
Coolbaugh
R C
RC
D000595:Amino Acid Sequence; D029681:Arabidopsis Proteins; D002736:Chloroplasts / Q000737:chemistry* / Q000235:genetics; D003001:Cloning, Molecular; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis* / Q000235:genetics; D018076:DNA, Complementary; D018744:DNA, Plant; D004926:Escherichia coli; D007425:Intracellular Membranes / Q000737:chemistry; D008969:Molecular Sequence Data; D018532:Pisum sativum / Q000737:chemistry* / Q000235:genetics; D010940:Plant Proteins / Q000032:analysis / Q000235:genetics
D029681:Arabidopsis Proteins; D018076:DNA, Complementary; D018744:DNA, Plant; D010940:Plant Proteins; D003577:Cytochrome P-450 Enzyme System; C438008:CYP86B1 protein, Arabidopsis