title: Efficient enantioselective separation of drug enantiomers by immobilised antibody fragments.
abstract: There is an increasing need for methods for efficient enantioselective separation and purification of chiral drugs. Genetic engineering provides the means for generating recombinant antibodies exhibiting extremely high specificity for even small molecular mass compounds. Here, recombinant antibody fragments have been generated for the drug diarylalkyltriazole that contains two chiral centres. Immobilised antibody fragments has been used successfully for efficient, step-wise separation of two enantiomers of the drug. Owing to the antibody specificity, one enantiomer came out in the flow-through, while the bound enantiomer could be specifically eluted. One of the antibodies tolerated solvents required both for dissolving the target molecules and for their elution for extended times and was shown to function over multiple cycles of the separation process.
authors.affiliation: VTT Biotechnology, Espoo, Finland.
authors.forename: T K
authors.initials: TK
authors.lastname: Nevanen
authors.forename: L
authors.initials: L
authors.lastname: Söderholm
authors.forename: K
authors.initials: K
authors.lastname: Kukkonen
authors.forename: T
authors.initials: T
authors.lastname: Suortti
authors.forename: T
authors.initials: T
authors.lastname: Teerinen
authors.forename: M
authors.initials: M
authors.lastname: Linder
authors.forename: H
authors.initials: H
authors.lastname: Söderlund
authors.forename: T T
authors.initials: TT
authors.lastname: Teeri
mesh_terms: D002846:Chromatography, Affinity / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D007128:Immunoglobulin Fragments / Q000737:chemistry*; D004364:Pharmaceutical Preparations / Q000737:chemistry / Q000302:isolation & purification*; D013237:Stereoisomerism
chemical_list: D007128:Immunoglobulin Fragments; D004364:Pharmaceutical Preparations