Heme oxygenase-1 regulates vascular tone of the peritoneum undergoing peritoneal dialysis.
The present study investigated the role of heme oxygenase-1 (HO-1) in regulating the vascular tone of the peritoneum undergoing peritoneal dialysis. First, we used reverse-transcriptase polymerase chain reaction (RT-PCR) to measure changes in the expression of HO-1 mRNA in the peritoneum of dehydrated Wistar Kyoto (WKY) rats. Second, we used a charge-coupled-device (CCD) camera to measure changes in the diameter and blood flow in the small artery of the peritoneum after intravenous administration of saline, hemin (4-12 mumol/kg), and tin-protoporphyrin (4-12 mumol/kg) in dehydrated WKY rats. In non dehydrated rats (control), no expression of HO-1 mRNA in the peritoneum was seen. In dehydrated and 10% glucose-treated dehydrated rats, HO-1 expression was significantly enhanced as compared with the control rats. Intravenous administration of saline induced no significant changes in the diameter of the small artery in dehydrated WKY rats. Intravenous administration of hemin induced a significant increase in the diameter of the small artery in dehydrated WKY rats. Intravenous administration of tin-protoporphyrin induced a significant reduction in the diameter of the small artery in dehydrated WKY rats. Our results suggest that 10% glucose injection into the peritoneal cavity induces overexpression of HO-1 in the peritoneum of dehydrated rats. In addition, HO-1 regulates peritoneal function by the regulation of vascular tone in the peritoneum.
Ishida
Y
Y
Department of Nephrology, Saitama Medical School, Saitama, Japan.
Nakamoto
H
H
Imai
H
H
Suzuki
S
S
Okano
H
H
Suzuki
H
H
D000818:Animals; D003681:Dehydration / Q000503:physiopathology; D005947:Glucose / Q000494:pharmacology; D006419:Heme Oxygenase (Decyclizing) / Q000032:analysis / Q000037:antagonists & inhibitors / Q000502:physiology*; D051547:Heme Oxygenase-1; D006427:Hemin / Q000494:pharmacology; D008297:Male; D008665:Metalloporphyrins / Q000494:pharmacology; D010537:Peritoneum / Q000098:blood supply* / Q000737:chemistry; D011524:Protoporphyrins / Q000494:pharmacology; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D011921:Rats, Inbred WKY; D012039:Regional Blood Flow; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014664:Vasodilation / Q000187:drug effects / Q000502:physiology*
D008665:Metalloporphyrins; D011524:Protoporphyrins; D012333:RNA, Messenger; D006427:Hemin; C032628:tin protoporphyrin IX; D006419:Heme Oxygenase (Decyclizing); D051547:Heme Oxygenase-1; D005947:Glucose