title: Corazonin gene expression in the waxmoth Galleria mellonella.
abstract: We cloned and sequenced a full length cDNA coding for [Arg7]-corazonin in the greater wax moth Galleria mellonella. The deduced corazonin preprohormone consists of a nineteen amino acid signal peptide, the actual eleven amino acid corazonin sequence, followed by a Gly serving for amidation, a Lys-Arg processing site and an eighty amino acid corazonin precursor-related peptide. The data confirm the phylogenetic conservation of the actual corazonin sequence. The signal peptide and the precursor-related peptide exhibit a similar spacing of a few amino acids as detected in the corazonin preprohormone of Drosophila melanogaster. Northern blots and in situ hybridization experiments revealed that the G. mellonella corazonin gene is tissue-specifically expressed in four pairs of lateral neurosecretory cells in the brains of penultimate and last instar larvae, as well as of pupae and adults. No corazonin mRNA was detected in other cells of the nervous system, fat body, gut, and several other organs.
authors.affiliation: Department of Cell- and Developmental Biology, Biocentre of the University, Am Hubland, D-97074 Würzburg, Germany.
authors.forename: I A
authors.initials: IA
authors.lastname: Hansen
authors.forename: F
authors.initials: F
authors.lastname: Sehnal
authors.forename: S R
authors.initials: SR
authors.lastname: Meyer
authors.forename: K
authors.initials: K
authors.lastname: Scheller
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary; D029721:Drosophila Proteins; D015870:Gene Expression; D019476:Insect Proteins; D008969:Molecular Sequence Data; D009036:Moths / Q000235:genetics / Q000378:metabolism; D009479:Neuropeptides / Q000096:biosynthesis / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution
chemical_list: C489221:Crz protein, Drosophila; D018076:DNA, Complementary; D029721:Drosophila Proteins; D019476:Insect Proteins; D009479:Neuropeptides; C060602:corazonin protein, insect