[Effect of vascular endothelial diphosphate glucuronosyltransferase mRNA regulated by alcohol].
OBJECTIVE
To investigate the effect of vascular endothelial growth factor(VEGF) gene transfection on tissue vascularization in transplanted site and the proliferation of transplanted hepatocytes.


METHODS
Samples were gained at ten days after the pcDNA3 VEGF121 gene transfected hepatocyte was transplanted into the spleen of SD rats. The changes of microvascular density(MVD) counts in the transplanted site and proliferating cell nuclear antigen(PCNA) index of the transplanted hepatocytes were examined.


RESULTS
In vitro protein expression of VEGF gene was enough to induce proliferation of human umbilical venous endothelial cells. In vivo VEGF gene transfected hepatocytes could form mass colonization of transplanted hepatocytes and reconstituted liver tissue. Difference of MVD counts in the transplanted site on all groups were not significant. P>0.05; PCNA index of pcDN-A3 VEGF121 gene transfection group increased significantly compared with pcDNA3 control group and no transfection group, P<0.01. PCNA index were 13.13 +/- 2.75, 4.75 +/- 1.58, and 4.63 +/- 1.41, suggesting that in vivo many factors affect the tissue vascularization, and expression of VEGF gene can promote not only proliferation of transplanted hepatocyte but formation of reconstituted liver tissue.


CONCLUSIONS
VEGF ex vivo gene transfection is an effective way to induce reconstitution of transplanted liver tissue.
Wang
J
J
Department of General Surgery, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University of Medical Sciences, GuangZhou, China.
Zhou
X
X
Min
J
J
D000818:Animals; D016228:Endothelial Growth Factors / Q000235:genetics / Q000502:physiology*; D005260:Female; D022781:Hepatocytes / Q000637:transplantation*; D008099:Liver / Q000098:blood supply; D008222:Lymphokines / Q000235:genetics / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014162:Transfection; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
D016228:Endothelial Growth Factors; D008222:Lymphokines; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors