Involvement of sigma(S) in starvation-induced transposition of Pseudomonas putida transposon Tn4652.
Transpositional activity of mobile elements can be induced by different environmental stresses. Here, we present evidence that transposition of Tn4652 is elevated in stationary-phase Pseudomonas putida and suppressed in an isogenic sigma(S)-defective strain. We demonstrate that transcription from the Tn4652 transposase promoter is controlled by the stationary-phase-specific sigma factor sigma(S). To our knowledge, this is the first example of direct stationary-phase-specific regulation of a mobile element transposase. Data presented in this report support the idea that activation of transposition under stressful conditions could be an inducible process.
Ilves
H
H
Estonian Biocentre and Institute of Molecular and Cell Biology, Tartu University, 51010 Tartu, Estonia.
Hõrak
R
R
Kivisaar
M
M
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D004251:DNA Transposable Elements; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D016958:Pseudomonas putida / Q000235:genetics* / Q000254:growth & development*; D012808:Sigma Factor / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic; D019895:Transposases / Q000235:genetics* / Q000378:metabolism
D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D012808:Sigma Factor; C122137:TnpC protein, Pseudomonas putida; D014157:Transcription Factors; C069567:sigma factor KatF protein, Bacteria; D019895:Transposases