An electrochemical multienzymatic biosensor for determination of cholesterol.
This paper describes an electrochemical biosensor for free cholesterol monitoring. The sensor is a multienzymatic electrodic system in which horseradish peroxidase and cholesterol oxidase are simultaneously immobilized within a polymeric film, on the surface of a pyrolitic graphite electrode. From voltammetric and amperometric (flow-injection) data obtained, the efficiency, reproducibility and stability of the system are discussed. Results obtained, of interest for basic and applied biochemistry, represent a first step for construction of a mediator-free biosensor with potentialities for a successful application in the biosensor area.
Bongiovanni
C
C
INFM e Dipartimento di Biologia, Università di Roma Tor Vergata, 00133 Rome, Italy.
Ferri
T
T
Poscia
A
A
Varalli
M
M
Santucci
R
R
Desideri
A
A
D015374:Biosensing Techniques; D002784:Cholesterol / Q000032:analysis* / Q000378:metabolism; D002789:Cholesterol Oxidase; D002942:Circular Dichroism; D004563:Electrochemistry / Q000295:instrumentation*; D004800:Enzymes, Immobilized; D017022:Flow Injection Analysis
D004800:Enzymes, Immobilized; D002784:Cholesterol; D002789:Cholesterol Oxidase