title: CueO is a multi-copper oxidase that confers copper tolerance in Escherichia coli.
abstract: The putative multi-copper oxidase CueO had previously been implicated in intrinsic copper resistance in Escherichia coli. In this report we showed that the presence of CueO in the periplasm protected alkaline phosphatase from copper-induced damage. CueO contained four copper atoms per molecule and displayed spectroscopic properties typical of blue copper oxidases. CueO catalyzed the oxidation of p-phenylenediamine (pPD), 2,6-dimethoxyphenol (DMP) and exhibited ferroxidase activity in vitro.
authors.affiliation: Department of Soil, Water, and Environmental Science, University of Arizona, Tucson, Arizona 85721, USA.
authors.forename: G
authors.initials: G
authors.lastname: Grass
authors.forename: C
authors.initials: C
authors.lastname: Rensing
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000595:Amino Acid Sequence; D002570:Ceruloplasmin / Q000378:metabolism; D003001:Cloning, Molecular; D003300:Copper / Q000737:chemistry* / Q000378:metabolism* / Q000494:pharmacology; D018844:Cyclin-Dependent Kinases / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology*; D029968:Escherichia coli Proteins; D015151:Immunoblotting; D007763:Lac Operon / Q000235:genetics; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000737:chemistry* / Q000378:metabolism / Q000502:physiology*; D010100:Oxygen / Q000378:metabolism; D019897:Periplasm / Q000201:enzymology; D010655:Phenylenediamines / Q000378:metabolism; D010957:Plasmids / Q000378:metabolism; D011748:Pyrogallol / Q000031:analogs & derivatives / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013053:Spectrophotometry; D013997:Time Factors; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D029968:Escherichia coli Proteins; D010655:Phenylenediamines; D011748:Pyrogallol; C010120:pyrogallol 1,3-dimethyl ether; D003300:Copper; D010088:Oxidoreductases; C499679:cueO protein, E coli; C024748:copper oxidase; D002570:Ceruloplasmin; D018844:Cyclin-Dependent Kinases; D000469:Alkaline Phosphatase; C114089:phoA protein, E coli; D001616:beta-Galactosidase; C029892:cupric chloride; D010100:Oxygen; C029728:4-phenylenediamine