Analysis of low-angle x-ray scattering peaks from lyophilized biological samples.
Low-angle x-ray scattering (LAXS) from lyophilized blood and its constituents is characterized by the presence of two peaks in the forward direction of scattering. These peaks are found to be sensitive to the variations in the molecular structure of a given sample. The present work aims to explore the nature of LAXS from a variety of lyophilized biological samples. It also aims to investigate the possibility that a certain biological macromolecule is responsible of the production of LAXS peaks. This is carried out through measurements of LAXS from complex biological samples and their basic constituents. Among the measured samples are haemoglobin (Hb), globin, haem, packed red blood cells, bovine albumin, egg albumin, milk, casein, glutamine, alanine, fat, muscle and DNA. A table containing some characteristic parameters of the LAXS profiles of these samples is also presented. Analysis of measured profiles shows that all lyophilized samples produce at least one relatively broad peak at a scattering angle around 10.35 degrees. The full width at half maximum (FWHM) of this peak varies considerably among the measured samples. Except for milk and casein. one additional peak at a scattering angle around 4.65 degrees is observed only in the LAXS profiles of proteins or protein-rich samples. This fact strongly suggests protein to be the biological macromolecule from which this characteristic peak originates. The same idea is further strengthened through discussion of some previous observations.
Desouky
O S
OS
Radiation Physics Department, National Center for Radiation Research and Technology, IAEA, Cairo, Egypt. omardesouky@yahoo.com
Elshemey
W M
WM
Selim
N S
NS
Ashour
A H
AH
D001774:Blood Chemical Analysis / Q000379:methods*; D001826:Body Fluids / Q000737:chemistry*; D004912:Erythrocytes / Q000737:chemistry; D005612:Freeze Drying; D005914:Globins / Q000032:analysis; D006454:Hemoglobins / Q000032:analysis; D006801:Humans; D012542:Scattering, Radiation; D012680:Sensitivity and Specificity; D012709:Serum Albumin / Q000032:analysis; D014965:X-Rays
D006454:Hemoglobins; D012709:Serum Albumin; D005914:Globins