Use of dyes to investigate migration of the chiral selector in CFFE and the impact on the chiral separations.
Continuous free flow electrophoresis was investigated as a tool for the preparative chiral separation of piperoxan enantiomers using sulfated beta-cyclodextrin (sbeta-CD) as the chiral additive. Bulk migration of sbeta-CD was confirmed using LC-MS analysis of the individual fractions collected and visualized with the addition of crystal violet to the separation buffer. In the absence of sbeta-CD, the crystal violet-containing buffer was reddish/purple and the crystal violet was deflected cathodically in the chamber. In the presence of sbeta-CD, the crystal violet-containing buffer was blue and was deflected anodically. However, formation of accumulation and depletion zones was apparent in both cases. The addition of sbeta-CD to the cathodic wash solution allowed for almost complete resolution of the piperoxan enantiomers with a processing rate of 0.45 mg/ h.
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Forensic Chemistry Center, FDA, Cincinnati, Ohio 45237-3097, USA.
Schneiderman
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Mertens
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Stalcup
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D004396:Coloring Agents / Q000737:chemistry*; D003505:Cyclodextrins / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D010883:Piperoxan / Q000737:chemistry*; D013237:Stereoisomerism; D047392:beta-Cyclodextrins
D004396:Coloring Agents; D003505:Cyclodextrins; D047392:beta-Cyclodextrins; D010883:Piperoxan; C031215:betadex