A region of the rat N-methyl-D-aspartate receptor 2A subunit that is sufficient for potentiation by phorbol esters.
N-methyl-D-aspartate (NMDA) receptors are modulated by protein kinase C (PKC) in vivo and in heterologous expression systems. In heterologous expression systems, PKC-mediated modulation is subunit specific with NR2A-containing receptors being potentiated by phorbol 12-myristate 13-acetate (PMA), while NR2C-containing receptors are inhibited or unaffected. In the present study we have produced chimeric receptors containing NR2A and NR2C to define the components of NR2A which are sufficient for potentiation by PMA. Amino acids 1105-1400 of NR2A placed onto the C-terminus of NR2C at amino acid 1102 was the minimum region sufficient for producing a PMA-stimulated receptor. This suggests that this region contains structural determinants for PKC-mediated potentiation of NR2A receptors.
Grant
E R
ER
Department of Neurology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA.
Guttmann
R P
RP
Seifert
K M
KM
Lynch
D R
DR
D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D006801:Humans; D007457:Iodine Radioisotopes; D010703:Phorbol Esters / Q000494:pharmacology*; D011493:Protein Kinase C / Q000378:metabolism; D016194:Receptors, N-Methyl-D-Aspartate / Q000819:agonists* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
D007457:Iodine Radioisotopes; C120997:NR2A NMDA receptor; D010703:Phorbol Esters; D016194:Receptors, N-Methyl-D-Aspartate; D011493:Protein Kinase C; D002118:Calcium