Structural variability and originality of the Bordetella endotoxins.
Structural studies of Bordetella endotoxins (LPSs) have revealed remarkable differences: (i) between their LPSs and those of other bacterial pathogens; (ii) among the LPSs of the seven identified Bordetella species; and (iii) among the LPSs of some Bordetella strains. The lipid As have the "classical" bisphosphorylated diglucosamine backbone but tend to have fewer and species-specific fatty acid components compared to those of other genera. Nevertheless, three strains of B. bronchiseptica have at least three different fatty acid distributions; however, the recently identified B. hinzii and B. trematum LPSs had identical lipid A structures. The B. pertussis core is a dodecasaccharide multi-branched structure bearing amino and carboxylic groups. Another unusual feature is the presence of free amino sugars in the central core region and a complex distal trisaccharide unit containing five amino groups of which four are acetylated and one is methylated. The B. pertussis LPS does not have O-chains and that of B. trematum had only a single O-unit, unlike the LPSs of all the other species of the smooth-type. The O-chain-free cores of non-B. pertussis LPSs were always built on the B. pertussis core model but most were species-specifically incomplete. The LPS structures of three B. bronchiseptica strains were found to be different from each other. The O-chains of B. bronchiseptica and B. parapertussis were almost identical and had some features in common with B. hinzii O-chain. Serological analyses are consistent with the determined LPS structures.
Caroff
M
M
Equipe 'Endotoxines', UMR 8619, Centre National de la Recherche Scientifique, Biochimie, Université de Paris-Sud, Orsay, France. martine.caroff@bbmpc.u-psud.fr
Aussel
L
L
Zarrouk
H
H
Martin
A
A
Richards
J C
JC
Thérisod
H
H
Perry
M B
MB
Karibian
D
D
D001884:Bordetella / Q000235:genetics / Q000276:immunology*; D002240:Carbohydrate Sequence; D002855:Chromatography, Thin Layer; D004591:Electrophoresis, Polyacrylamide Gel; D004731:Endotoxins / Q000737:chemistry* / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008050:Lipid A / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods; D008969:Molecular Sequence Data; D015394:Molecular Structure
D004731:Endotoxins; D005227:Fatty Acids; D008050:Lipid A