Nuclear non-histone protein p36 associated with colorectal tumours.
Rabbit serum raised against electrophoretically specific nuclear polypeptides with molecular weights of 35-40 kD from colon adenocarcinoma has been used to detect p36 antigen in 83.3% (40 of 48) of cases of large intestine tumours by means of Western blot technique. Immunological analysis revealed that this antiserum cross-reacted with antigen of the same molecular weight in 83.3% (10 of 12) and 85.7% (6 of 7) nuclear protein preparations from stomach and lung tumours, respectively, but not in any control tissue samples. No cross-reactivity within the region of 36 kD was observed among nuclear proteins isolated from mononuclear cells of B-cell chronic lymphocytic leukaemia patients as well as healthy donors.
Kiliańska
Z M
ZM
Department of Cytobiochemistry, University of łódź, Poland.
Szymczyk
P
P
Krajewska
W M
WM
Jakubik
J
J
Błoński
J
J
Berner
J
J
Robak
T
T
D000818:Animals; D000918:Antibody Specificity; D000951:Antigens, Neoplasm / Q000276:immunology*; D014408:Biomarkers, Tumor / Q000276:immunology; D015179:Colorectal Neoplasms / Q000175:diagnosis / Q000276:immunology*; D003429:Cross Reactions; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D015151:Immunoblotting; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000276:immunology; D008175:Lung Neoplasms / Q000276:immunology; D008970:Molecular Weight; D009363:Neoplasm Proteins / Q000276:immunology; D009687:Nuclear Proteins / Q000276:immunology*; D011817:Rabbits; D013274:Stomach Neoplasms / Q000276:immunology
D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D007106:Immune Sera; D009363:Neoplasm Proteins; D009687:Nuclear Proteins