title: Integration of porcine chromosome 13 maps.
abstract: In order to expand the comparative map between human chromosome 3 (HSA3) and porcine chromosome 13 (SSC13), seven genes from HSA3 were mapped on SSC13 by fluorescence in situ hybridisation (FISH), viz. ACAA1, ACPP, B4GALT4, LTF, MYLK, PDHB and RARB. With a view to integrating this expanded comparative map with the existing SSC13 linkage map, we used the INRA-University of Minnesota porcine Radiation Hybrid panel (IMpRH) to localize more precisely and to order 15 genes on the SSC13 map, viz. ACPP, ADCY5, APOD, BCHE, CD86, DRD3, GAP43, PCCB, RAF1, RHO, SI, TF, TFRC, TOP2B and ZNF148. In this way, we were able to create an integrated map, containing 38 type I and 81 type II markers, by correlating the linkage, radiation hybrid (RH) and cytogenetic maps of SSC13. This integrated map will give us the opportunity to take maximal advantage of the comparative mapping strategy for positional candidate cloning of genes responsible for economically important traits.
authors.affiliation: Department of Animal Nutrition, Genetics, Breeding and Ethology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
authors.forename: M
authors.initials: M
authors.lastname: Van Poucke
authors.forename: M
authors.initials: M
authors.lastname: Yerle
authors.forename: C
authors.initials: C
authors.lastname: Tuggle
authors.forename: F
authors.initials: F
authors.lastname: Piumi
authors.forename: C
authors.initials: C
authors.lastname: Genêt
authors.forename: A
authors.initials: A
authors.lastname: Van Zeveren
authors.forename: L J
authors.initials: LJ
authors.lastname: Peelman
mesh_terms: D000818:Animals; D002875:Chromosomes / Q000235:genetics*; D022202:Chromosomes, Artificial, Bacterial; D003001:Cloning, Molecular; D005796:Genes; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008126:Lod Score; D008969:Molecular Sequence Data; D020161:Physical Chromosome Mapping; D016133:Polymerase Chain Reaction; D021101:Radiation Hybrid Mapping; D013552:Swine / Q000235:genetics*