title: Studies of posttranslational modifications in spiny dogfish myelin basic protein.
abstract: The objective of this investigation was to determine whether nonmammalian myelin basic protein contained charge isomers resulting from extensive posttranslational modifications as seen in mammalian MBP. Four charge isomer components from dogfish MBP have been isolated. These forms arise by phosphorylation and deamidation modifications. Components C1, C2 and C3 have been characterized. We are currently characterizing component C8. Dogfish MBP is less cationic than mammalian MBP and has about 50% lower mobility on a basic pH gel electrophoresis relative to human and to bovine MBP. The mammalian component C1, which is unmodified, is modified in the dogfish by phosphorylation. The reduced electrophoretic mobility is largely attributable to the charge reduction resulting from phosphorylation in serine 72, 83, and 120 or 121 in C1, and C3. In component C2, two or three phosphate groups were distributed among residues 134, 138 and 139. It was found that dogfish amino acid residue 30 was a lysine residue and not a glutamate residue as reported in the literature.
authors.affiliation: Department of Biological Chemistry, University of Michigan, Ann Arbor 48109-1055, USA.
authors.forename: R
authors.initials: R
authors.lastname: Zand
authors.forename: X
authors.initials: X
authors.lastname: Jin
authors.forename: J
authors.initials: J
authors.lastname: Kim
authors.forename: D B
authors.initials: DB
authors.lastname: Wall
authors.forename: R
authors.initials: R
authors.lastname: Gould
authors.forename: D M
authors.initials: DM
authors.lastname: Lubman
mesh_terms: D000595:Amino Acid Sequence / Q000235:genetics; D000818:Animals; D004284:Dogfish / Q000378:metabolism*; D004563:Electrochemistry; D007536:Isomerism; D008969:Molecular Sequence Data; D008970:Molecular Weight; D004676:Myelin Basic Protein / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational
chemical_list: D004676:Myelin Basic Protein