title: [The Thermus thermophilus 5S rRNA-protein complex: Identifications of specific binding sites for proteins L5 and L18 in 5S rRNA].
abstract: Three 5S rRNA-binding ribosomal proteins (L5, L18, TL5) of extremely thermophilic bacterium Thermus thermophilus have earlier been isolated. Structural analysis of their complexes with rRNA requires identification of their binding sites in the 5S rRNA. Previously, a TL5-binding site has been identified, a TL5-RNA complex crystallized, and its structure determined to 2.3 A. The sites for L5 and L18 were characterized, and two corresponding 5S rRNA fragments constructed. Of these, a 34-nt fragment specifically interacted with L5, and a 55-nt fragment interacted with L5, L18, and with both proteins. The 34-nt fragment-L5 complex was crystallized; the crystals are suitable for high-resolution X-ray analysis.
authors.affiliation: gongadze@vega.protres.ru
authors.forename: G M
authors.initials: GM
authors.lastname: Gongadze
authors.forename: A A
authors.initials: AA
authors.lastname: Perederina
authors.forename: V A
authors.initials: VA
authors.lastname: Meshcheriakov
authors.forename: R V
authors.initials: RV
authors.lastname: Fedorov
authors.forename: S E
authors.initials: SE
authors.lastname: Moskalenko
authors.forename: A V
authors.initials: AV
authors.lastname: Rak
authors.forename: A A
authors.initials: AA
authors.lastname: Serganov
authors.forename: D V
authors.initials: DV
authors.lastname: Shcherbakov
authors.forename: S V
authors.initials: SV
authors.lastname: Nikonov
authors.forename: M B
authors.initials: MB
authors.lastname: Garber
mesh_terms: D001665:Binding Sites; D011485:Protein Binding; D012329:RNA, Bacterial / Q000235:genetics / Q000378:metabolism; D012341:RNA, Ribosomal, 5S / Q000235:genetics / Q000378:metabolism*; D012269:Ribosomal Proteins / Q000235:genetics / Q000378:metabolism*; D016963:Thermus thermophilus / Q000235:genetics* / Q000378:metabolism*
chemical_list: D012329:RNA, Bacterial; D012341:RNA, Ribosomal, 5S; D012269:Ribosomal Proteins; C029609:ribosomal protein L18; C029742:ribosomal protein L5
vernacular_title: 5S rRNK-belkovyĭ kompleks Thermus thermophilus. Opredelenie spetsificheskikh uchastkov sviazyvaniia belkov L5 i L18 na 5S rRNK.