title: [Study of molecular mechanisms of translation initiation in mammals using in vitro reconstruction of initiation complexes].
abstract: Papers on the mechanisms of translation initiation in mammals studied by reconstruction of initiation complexes from individual components are reviewed. The author points to the constraints of this approach and to the pitfalls ignoring which one might come to erroneous conclusions and even artifacts. In addition, some methods employed in the field as well as some technical problems are discussed in the paper, together with the means of obviating them. The review could be a guidebook for newcomers into this quite labor-consuming field.
authors.affiliation: shatsky@libro.genebee.msu.su
authors.forename: I N
authors.initials: IN
authors.lastname: Shatskiĭ
mesh_terms: D000818:Animals; D008322:Mammals; D010442:Peptide Chain Initiation, Translational; D010448:Peptide Initiation Factors / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D012335:RNA, Ribosomal / Q000235:genetics
chemical_list: D010448:Peptide Initiation Factors; D012333:RNA, Messenger; D012335:RNA, Ribosomal
vernacular_title: Rekonstruktsiia initsiiruiushchikh kompleksov in vitro dlia issledovaniia molekuliarnykh mekhanizmov initsiatsii transliatsii u mlekopitaiushchikh.