title: Reactivity of MEST-1 (antigalactofuranose) with Trypanosoma cruzi glycosylinositol phosphorylceramides (GIPCs): immunolocalization of GIPCs in acidic vesicles of epimastigotes.
abstract: Using confocal microscopy, MEST-1-positive immunofluorescence was observed within various Trypanosoma cruzi forms, except in cell-derived trypomastigotes. Glycosylinositol phosphorylceramides were identified by thin-layer chromatography immunostaining as the antigens recognized by MEST-1 in these parasites. In epimastigotes, labeling of MEST-1 coincided with acidic vesicles, indicating an internal localization of these glycoconjugates.
authors.affiliation: Departamento de Bioquímica, Universidade Federal de São Paulo, Escola Paulista de Medicina, Rua Botucatu 862, São Paulo, SP 04023-900, Brazil.
authors.forename: E
authors.initials: E
authors.lastname: Suzuki
authors.forename: R A
authors.initials: RA
authors.lastname: Mortara
authors.forename: H K
authors.initials: HK
authors.lastname: Takahashi
authors.forename: A H
authors.initials: AH
authors.lastname: Straus
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism*; D000913:Antibodies, Protozoan / Q000378:metabolism; D000953:Antigens, Protozoan / Q000276:immunology / Q000378:metabolism; D005663:Furans / Q000276:immunology*; D005690:Galactose / Q000276:immunology*; D006028:Glycosphingolipids / Q000276:immunology* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D051379:Mice; D022161:Transport Vesicles / Q000276:immunology* / Q000378:metabolism; D014349:Trypanosoma cruzi / Q000254:growth & development* / Q000276:immunology* / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D005663:Furans; D006028:Glycosphingolipids; C025524:inositolphosphoceramides; D005690:Galactose