On-line identification of trans- and cis-resveratrol by nonaqueous capillary electrophoresis/fluorescence spectroscopy at 77 K.
This work presents a novel method for the accurate determining trans- and cis-resveratrol (3,5,4'-trihydroxystilbene) by nonaqueous capillary electrophoresis/fluorescence spectroscopy at 77 K. The proposed method permits not only the separation of resveratrol isomers, but also ensures that on-line spectra are readily distinguishable and unambiguously assigned. The experimental results also indicate that the effect of nonaqueous capillary electrophoresis buffer and low-temperature technique increase the detection limit by more than 150-fold.
Lin
C H
CH
Department of Chemistry, National Taiwan Normal University, Taipei. chenglin@cc.ntnu.edu.tw
Chen
Y H
YH
D000085:Acetates; D000097:Acetonitriles; D003080:Cold Temperature; D019075:Electrophoresis, Capillary / Q000379:methods*; D007536:Isomerism; D000432:Methanol; D010777:Photochemistry; D000077185:Resveratrol; D020358:Sodium Cholate; D012995:Solubility; D012997:Solvents; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013267:Stilbenes / Q000032:analysis* / Q000737:chemistry / Q000528:radiation effects
D000085:Acetates; D000097:Acetonitriles; D012997:Solvents; D013267:Stilbenes; D020358:Sodium Cholate; D000077185:Resveratrol; C018824:ammonium acetate; D000432:Methanol; C032159:acetonitrile