title: Crystallization of a complex between human CDK6 and a virus-encoded cyclin is critically dependent on the addition of small charged organic molecules.
abstract: Human CDK6 plays an important role in controlling entry into the eukaryotic cell cycle. An activated complex of human CDK6 with a viral cyclin from herpesvirus saimiri was purified to homogeneity and crystallized using polyethylene glycol 3350 as precipitant. Crystallization was critically dependent on a narrow range of calcium acetate concentration and the presence of sulfo-betaine 201 as additive. Crystals belong to the hexagonal space group P6(1)22 or P6(5)22, with unit-cell parameters a = b = 70.14, c = 448.77 A, gamma = 120 degrees, and diffract X-rays to at least 3.1 A resolution.
authors.affiliation: Department of Chemistry and Earnest Orlando Berkeley National Laboratory, University of California, Berkeley, California 94720, USA. uschulze-gahmen@lbl.gov
authors.forename: U
authors.initials: U
authors.lastname: Schulze-Gahmen
authors.forename: S H
authors.initials: SH
authors.lastname: Kim
mesh_terms: D000085:Acetates / Q000737:chemistry*; D017610:Calcium Compounds; D003460:Crystallization; D018360:Crystallography, X-Ray; D051361:Cyclin-Dependent Kinase 6; D018844:Cyclin-Dependent Kinases; D016213:Cyclins / Q000737:chemistry*; D006567:Herpesvirus 2, Saimiriine / Q000737:chemistry; D006801:Humans; D011487:Protein Conformation; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry*; D014764:Viral Proteins / Q000737:chemistry*
chemical_list: D000085:Acetates; D017610:Calcium Compounds; D016213:Cyclins; D014764:Viral Proteins; D017346:Protein Serine-Threonine Kinases; C495911:CDK6 protein, human; D051361:Cyclin-Dependent Kinase 6; D018844:Cyclin-Dependent Kinases; C120662:calcium acetate