PH-Zone-refining counter-current chromatography of lappaconitine from Aconitum sinomontanum nakai I. Separation of prepurified extract.
pH-Zone-refining counter-current chromatography was applied to the separation of diterpenoid alkaloids from a crude sample from a crude prepurified sample containing lappaconitine at about 90% purity using a multilayer coil planet centrifuge. The experiment was performed with a two-phase solvent system composed of methyl tert.-butyl ether-tetrahydrofuran-distilled water (2:2:3, v/v) where triethylamine (10 mM) was added to the upper organic stationary phase as a retainer and hydrochloric acid (10 mM) to the aqueous mobile phase as an eluter. The separation of 10.5 g of the sample yielded 9.0 g of lappaconitine at a high purity of over 99% as determined by HPLC.
Yang
F
F
Laboratory of Biophysical Chemistry, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA.
Ito
Y
Y
D000157:Aconitine / Q000031:analogs & derivatives* / Q000302:isolation & purification*; D002681:China; D006863:Hydrogen-Ion Concentration; D010946:Plants, Medicinal / Q000737:chemistry; D029626:Ranunculaceae / Q000737:chemistry*
C110231:lappaconidine; D000157:Aconitine