title: Spontaneous regression over time of the germinal epithelium in a Y chromosome-microdeleted patient: Case report.
abstract: Azoospermia factor (AZF) region microdeletions, which account for about 10-15% of patients with oligoazoospermia, seem to lack a close genotype-testicular phenotype correlation. Although many genetic and non-genetic factors may contribute to this outcome, it was thought that a spontaneous regression of testicular germ cells might also play a relevant role. The opportunity for carrying out two different testicular biopsies one year apart in an AZFc-microdeleted patient enabled corroboration of this possibility. Indeed, the first biopsy showed a spermatocyte maturation arrest with mean Johnsen scores of 4 and 3.9 in the right and left testes respectively. One year later, the right testicular biopsy showed a picture of Sertoli cell-only syndrome in 90% of the tubules examined, and of spermatogonial maturation arrest in the remaining tubules, with a mean Johnsen score of 2.1. The almost complete absence of germinal cells was confirmed by four left testicular sperm aspirations (TESA), conducted at the same time as the biopsy during an intracytoplasmic sperm injection cycle, which showed the almost exclusive presence of Sertoli cells (85% of the whole cell population). No spermatozoa could be retrieved by TESA or testicular biopsy. To our knowledge, this is the first case of a spontaneous regression of the germinal cell epithelium over time in a patient with a Yq microdeletion without the apparent intervention of any cause known to affect the germinal epithelium.
authors.affiliation: Division of Endocrinology, New Methodologies in Human Reproductive Medicine, Department of Internal Medicine, University of Catania, Catania, Italy. acaloger@unict.it
authors.forename: A E
authors.initials: AE
authors.lastname: Calogero
authors.forename: M R
authors.initials: MR
authors.lastname: Garofalo
authors.forename: N
authors.initials: N
authors.lastname: Barone
authors.forename: A
authors.initials: A
authors.lastname: De Palma
authors.forename: E
authors.initials: E
authors.lastname: Vicari
authors.forename: R
authors.initials: R
authors.lastname: Romeo
authors.forename: S
authors.initials: S
authors.lastname: Tumino
authors.forename: R
authors.initials: R
authors.lastname: D'Agata
mesh_terms: D000328:Adult; D001706:Biopsy; D016922:Cellular Senescence; D004847:Epithelial Cells / Q000473:pathology; D017353:Gene Deletion; D056426:Genetic Loci; D005854:Germ Cells / Q000473:pathology; D006801:Humans; D008297:Male; D009845:Oligospermia / Q000235:genetics* / Q000473:pathology*; D029589:Seminal Plasma Proteins / Q000235:genetics; D012671:Seminiferous Tubules / Q000473:pathology; D012708:Sertoli Cells / Q000473:pathology; D013076:Sperm Count; D013090:Spermatocytes / Q000502:physiology; D013737:Testis / Q000473:pathology*; D013997:Time Factors; D014998:Y Chromosome / Q000235:genetics*
chemical_list: D029589:Seminal Plasma Proteins