title: Effect of the herbicide 4-CPA on human erythrocyte antioxidant enzymes in vitro.
abstract: To investigate the possible role of oxygen free radicals and oxidant stress in the toxic effects of phenoxyherbicides, we studied the in vitro effect of 4-chlorophenoxyacetic acid (4-CPA) on various human erythrocyte antioxidant enzymes, namely glucose-6-phosphate dehydrogenase, catalase, selenium-dependent glutathione peroxidase, glutathione reductase and Cu/Zn-superoxide dismutase. 4-CPA added in a dose of 1 ppm to human erythrocytes for 1 h caused a significant reduction in glucose-6-phosphate dehydrogenase (P <0.001) and catalase (P <0.001) activities, but did not significantly affect the activities of other enzymes. Such selective inactivation of specific erythrocyte antioxidant enzymes may play a role in the toxic effects of phenoxyherbicides.
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Akdeniz University, Arapsuyu, Antalya, Turkey. yakup@hipokrat.med.akdeniz.edu.tr
authors.forename: Y
authors.initials: Y
authors.lastname: Alicigüzel
authors.forename: S
authors.initials: S
authors.lastname: Ozdem
authors.forename: A Y
authors.initials: AY
authors.lastname: Demir
authors.forename: F
authors.initials: F
authors.lastname: Unal
authors.forename: D
authors.initials: D
authors.lastname: Kumbul
authors.forename: S S
authors.initials: SS
authors.lastname: Ozdem
authors.forename: G
authors.initials: G
authors.lastname: Perry
authors.forename: M A
authors.initials: MA
authors.lastname: Smith
mesh_terms: D015084:2,4-Dichlorophenoxyacetic Acid / Q000031:analogs & derivatives / Q000494:pharmacology*; D000975:Antioxidants / Q000032:analysis*; D002374:Catalase / Q000037:antagonists & inhibitors* / Q000097:blood; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D004912:Erythrocytes / Q000187:drug effects* / Q000201:enzymology; D005609:Free Radicals; D005954:Glucosephosphate Dehydrogenase / Q000037:antagonists & inhibitors* / Q000097:blood; D005979:Glutathione Peroxidase / Q000037:antagonists & inhibitors* / Q000097:blood; D005980:Glutathione Reductase / Q000037:antagonists & inhibitors* / Q000097:blood; D006540:Herbicides / Q000494:pharmacology*; D006801:Humans; D018384:Oxidative Stress; D012643:Selenium; D013482:Superoxide Dismutase / Q000037:antagonists & inhibitors* / Q000097:blood
chemical_list: D000975:Antioxidants; D004791:Enzyme Inhibitors; D005609:Free Radicals; D006540:Herbicides; D015084:2,4-Dichlorophenoxyacetic Acid; C007017:4-chlorophenoxyacetic acid; D005954:Glucosephosphate Dehydrogenase; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase; D012643:Selenium