Preparation and characterization of monolithic polymer columns for capillary electrochromatography.
A series of micro-monolithic columns with different porosities were prepared for capillary electrochromatography (CEC) by in-situ copolymerization of butyl methacrylate, ethylene glycol dimethacrylate, and 2-acrylamido-2-methyl-1-propane-sulfonic acid in the presence of a porogen in fused-silica capillaries of 100 microm I.D. Different column porosities were obtained by changing the ratios of monomers to porogenic solvents. Columns were investigated and evaluated under both pressure-driven (high-performance liquid chromatography, HPLC) and electro-driven (capillary electrochromatography, CEC) conditions. Each column exhibited different efficiency and dependency on flow velocity under electro-driven conditions. Abnormally broad peaks for some relatively bulky molecules were observed. Possible explanations are discussed. The differences in column efficiency and retention behavior between the two eluent-driven modes were studied in detail. In addition, other column properties, such as morphology, porosity, stability and reproducibility, were extensively tested.
Jiang
T
T
Department of Chemical Engineering and Chemistry, Eindhoven University of Technology, The Netherlands.
Jiskra
J
J
Claessens
H A
HA
Cramers
C A
CA
D002845:Chromatography / Q000295:instrumentation*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation; D011108:Polymers; D015203:Reproducibility of Results
D011108:Polymers