HACS1 encodes a novel SH3-SAM adaptor protein differentially expressed in normal and malignant hematopoietic cells.
SH3 and SAM domains are protein interaction motifs that are predominantly seen in signaling molecules, adaptors, and scaffold proteins. We have identified a novel family of putative adaptor genes that includes HACS1. HACS1 encodes a 441 amino acid protein that is differentially expressed in hematopoietic cells and has restricted expression in human tissues. Its SH3 domain is most similar to the same motif in Crk and its SAM domain shares homology with a family of uncharacterized putative scaffold and adaptor proteins. HACS1 maps to human chromosome 21q11.2 in a region that is frequently disrupted by translocation events in hematopoietic malignancies. Polyclonal antibodies against HACS1 recognized a 49.5 kDa protein whose mRNA is expressed in human immune tissues, bone marrow, heart, lung, placenta and brain. Cell lines and primary cells from acute myeloid leukemias and multiple myeloma patients express HACS1. Immunostaining and cellular fractionation studies localized the HACS1 protein predominantly to the cytoplasm.
Claudio
J O
JO
Experimental Therapeutics, Toronto General Research Institute, Toronto, Ontario, Canada.
Zhu
Y X
YX
Benn
S J
SJ
Shukla
A H
AH
McGlade
C J
CJ
Falcioni
N
N
Stewart
A K
AK
D033942:Adaptor Proteins, Vesicular Transport; D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000378:metabolism; D015152:Blotting, Northern; D019556:COS Cells; D002891:Chromosomes, Human, Pair 21; D003593:Cytoplasm / Q000378:metabolism; D019337:Hematologic Neoplasms / Q000378:metabolism*; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction; D013347:Subcellular Fractions; D013997:Time Factors; D014018:Tissue Distribution; D014162:Transfection; D014178:Translocation, Genetic; D018909:src Homology Domains
D033942:Adaptor Proteins, Vesicular Transport; D000906:Antibodies; D011506:Proteins; C435526:SAMSN1 protein, human