Determination of sameridine in blood plasma by nitrogen-selective gas chromatography.
A gas chromatographic method was developed and validated for the determination of sameridine in human plasma. Sameridine is a new type of compound with both local anaesthetic and analgesic properties, when administered intrathecally. The method is based on liquid-liquid extraction of sameridine from 1.0 ml of plasma, followed by gas chromatography with nitrogen-phosphorus detection. Method validation results showed that this method is very sensitive, selective and robust. The limit of quantification was 1 nM for 1.0 ml of human plasma in the low-level range (1.00-75.0 nM) and the between-day accuracy and precision were measured at 99-104% of nominal values and 3.4-5.6% (RSD), respectively.
Norsten-Höög
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C
Bioanalytical Chemistry, AstraZeneca R&D Södertälje, Sweden. carina.norsten-hoog@astrazeneca.com
Neidenström
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P
Arvidsson
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T
D000700:Analgesics / Q000097:blood* / Q000493:pharmacokinetics; D000779:Anesthetics, Local / Q000097:blood* / Q000493:pharmacokinetics; D002849:Chromatography, Gas / Q000379:methods*; D006801:Humans; D009584:Nitrogen; D010880:Piperidines / Q000097:blood* / Q000493:pharmacokinetics; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000700:Analgesics; D000779:Anesthetics, Local; D010880:Piperidines; D009584:Nitrogen; C113809:sameridine