Effects of cyclodextrins on the complexation between Methylene Blue and tetrakis(4-sulfonatophenyl)porphyrin in aqueous solutions.
In aqueous solutions buffered at pH 10.1, Methylene Blue (MB) forms a complex with tetrakis(4-sulfonatophenyl)porphyrin (TSPP). The equilibrium constant for the formation of the MB-TSPP complex has been evaluated to be 2.35 x 10(5) mol(-1) dm3 from the fluorescence quenching of MB by TSPP. Effects of alpha-, beta-, and gamma-cyclodextrin (CD), and heptakis(2,3,6-tri-O-methyl)-beta-cyclodextrin on the complexation between MB and TSPP have been examined by means of absorption and fluorescence spectroscopy. The binding of CDs to TSPP and/or MB in the MB-TSPP complex causes the dissociation of the MB-TSPP complex.
Hamai
S
S
Department of Chemistry, Faculty of Education and Human Studies, Akita University, Japan. hamai@ipc.akita-u.ac.jp
Satou
H
H
D002021:Buffers; D003505:Cyclodextrins / Q000737:chemistry*; D004791:Enzyme Inhibitors / Q000737:chemistry; D008751:Methylene Blue / Q000737:chemistry*; D015394:Molecular Structure; D009282:Naphthalenesulfonates / Q000737:chemistry*; D011166:Porphyrins / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D014867:Water / Q000737:chemistry
C446699:1-naphthol-2-sulfonate; D002021:Buffers; D003505:Cyclodextrins; D004791:Enzyme Inhibitors; D009282:Naphthalenesulfonates; D011166:Porphyrins; D014867:Water; D008751:Methylene Blue