Gp120 activates children's brain endothelial cells via CD4.
Encephalopathy represents a common and serious manifestation of HIV-1 infection in children, but its pathogenesis is unclear. We demonstrated that gp120 activated human brain microvascular endothelial cells (HBMEC) derived from children in up-regulating ICAM-1 and VCAM-1 expression, IL-6 secretion and increased monocyte transmigration across monolayers. Another novel observation was our demonstration of CD4 in isolated HBMEC and on microvessels of children's brain cryosections. Gp120-induced monocyte migration was inhibited by anti-gp120 and anti-CD4 antibodies. This is the first demonstration that gp120 activates HBMEC via CD4, which may contribute to the development of HIV-1 encephalopathy in children.
Stins
M F
MF
Division of Infectious Diseases, Childrens Hospital Los Angeles, California, USA.
Shen
Y
Y
Huang
S H
SH
Gilles
F
F
Kalra
V K
VK
Kim
K S
KS
D015526:AIDS Dementia Complex / Q000378:metabolism / Q000473:pathology / Q000821:virology*; D000328:Adult; D000906:Antibodies / Q000494:pharmacology; D001921:Brain / Q000098:blood supply / Q000473:pathology / Q000821:virology*; D015704:CD4 Antigens / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D002465:Cell Movement / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D004730:Endothelium, Vascular / Q000737:chemistry / Q000378:metabolism / Q000821:virology*; D005434:Flow Cytometry; D005629:Frozen Sections; D015699:HIV Envelope Protein gp120 / Q000276:immunology / Q000378:metabolism*; D015497:HIV-1; D006801:Humans; D066298:In Vitro Techniques; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis / Q000096:biosynthesis; D009000:Monocytes / Q000166:cytology / Q000276:immunology / Q000821:virology; D012333:RNA, Messenger / Q000032:analysis; D019010:Vascular Cell Adhesion Molecule-1 / Q000032:analysis / Q000096:biosynthesis
D000906:Antibodies; D015704:CD4 Antigens; D015699:HIV Envelope Protein gp120; D012333:RNA, Messenger; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1