Short report: Antibody responses of mice immunized with a tetravalent dengue recombinant protein subunit vaccine.
Recombinant proteins containing the B domain of dengue virus serotypes 1-4 fused to the maltose binding protein (MBP) of Escherichia coli were evaluated individually and as a tetravalent vaccine candidate in mice. Sera from mice immunized with monovalent DEN-MBP recombinant protein vaccines developed high titers of serotype homologous antibody in the enzyme-linked immunosorbent assay and the plaque-reduction neutralization test. Cross-reactive antibody titers were either several dilutions lower or not detectable. Sera from mice immunized with the tetravalent DEN subunit vaccine neutralized all 4 DEN viruses in the plaque-reduction neutralization test. The neutralizing antibody titers to each individual serotype were significantly greater than any cross-reactive neutralizing antibody titers induced by the monovalent vaccines, providing evidence that the tetravalent DEN recombinant subunit vaccine produced specific neutralizing antibody to all 4 serotypes of dengue virus.
Simmons
M
M
Virology Program, Naval Medical Research Center, Silver Spring, Maryland 20910-7500, USA. simmonsm@nmrc.navy.mil
Murphy
G S
GS
Hayes
C G
CG
D018528:ATP-Binding Cassette Transporters; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D001426:Bacterial Proteins / Q000235:genetics; D002352:Carrier Proteins / Q000235:genetics; D003429:Cross Reactions; D003715:Dengue / Q000517:prevention & control*; D003716:Dengue Virus / Q000235:genetics / Q000276:immunology*; D004926:Escherichia coli; D029968:Escherichia coli Proteins; D007114:Immunization; D058619:Maltose-Binding Proteins; D051379:Mice; D008807:Mice, Inbred BALB C; D009004:Monosaccharide Transport Proteins; D009500:Neutralization Tests; D011993:Recombinant Fusion Proteins / Q000276:immunology; D017778:Vaccines, Combined; D022223:Vaccines, Subunit / Q000276:immunology; D014614:Vaccines, Synthetic; D014764:Viral Proteins / Q000235:genetics; D014765:Viral Vaccines / Q000276:immunology
D018528:ATP-Binding Cassette Transporters; D000914:Antibodies, Viral; D001426:Bacterial Proteins; D002352:Carrier Proteins; D029968:Escherichia coli Proteins; D058619:Maltose-Binding Proteins; D009004:Monosaccharide Transport Proteins; D011993:Recombinant Fusion Proteins; D017778:Vaccines, Combined; D022223:Vaccines, Subunit; D014614:Vaccines, Synthetic; D014764:Viral Proteins; D014765:Viral Vaccines; C461599:maltose transport system, E coli