Estrogenic activity of phenolic compounds from Nigella damascena evaluated using a recombinant yeast screen.
We used a yeast estrogen screen (YES) containing human estrogen receptor to evaluate the estrogenic activity of both crude extracts and simple pure phenolic compounds from Nigella damascena seeds. Estrogenic activity was established in the methanolic and aqueous extracts of the seeds as well as in two simple phenolic compounds isolated from the methanolic extract, 2,4-dihydroxyphenylacetic acid, 3,4-dihydroxy-beta-phenethyl alcohol.
Agradi
E
E
Fico
G
G
Cillo
F
F
Francisci
C
C
Tomè
F
F
D003115:Colony-Stimulating Factors / Q000187:drug effects; D004967:Estrogens / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D007763:Lac Operon; D019684:Magnoliopsida / Q000737:chemistry*; D010648:Phenylacetates / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology; D010946:Plants, Medicinal / Q000737:chemistry; D011960:Receptors, Estrogen / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins; D012441:Saccharomyces cerevisiae
D003115:Colony-Stimulating Factors; D004967:Estrogens; D010648:Phenylacetates; D010936:Plant Extracts; D011960:Receptors, Estrogen; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; C005975:3,4-dihydroxyphenylethanol; C054012:2,4-dihydroxyphenylacetic acid; D010626:Phenylethyl Alcohol