D-fructose-binding proteins in bull seminal plasma: isolation and characterization.
The heparin-binding activity of bull seminal plasma proteins was inhibited by D-fructose, D-glucose, inulin and glycogen; D-galactose, dextran and mannan had no effect. While the ejaculated sperm-heparin interaction was not influenced by the presence of saccharides, the heparin-binding activity of epididymal sperm was inhibited by D-fructose. The results of the binding studies were confirmed by affinity chromatography on immobilized heparin followed by elution with monosaccharides. Proteins adsorbed to a heparin-polyacrylamide column and eluted with D-fructose were analyzed by RP HPLC, SDS electrophoresis and by determination of the N-terminal amino-acid sequence. RNAase dimer, PDC-109 and metalloproteinase inhibitor (TIMP-2) were identified.
Liberda
J
J
Department of Biochemistry, Charles University, Prague, Czech Republic.
Kraus
M
M
Ryslavá
H
H
Vlasáková
M
M
Jonáková
V
V
Tichá
M
M
D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D002417:Cattle / Q000378:metabolism*; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D003911:Dextrans / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D004822:Epididymis / Q000378:metabolism; D005632:Fructose / Q000378:metabolism* / Q000494:pharmacology; D005690:Galactose / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology; D006003:Glycogen / Q000494:pharmacology; D006493:Heparin / Q000378:metabolism*; D007444:Inulin / Q000494:pharmacology; D008297:Male; D008351:Mannans / Q000494:pharmacology; D008969:Molecular Sequence Data; D011485:Protein Binding / Q000187:drug effects; D012260:Ribonucleases / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D012661:Semen / Q000737:chemistry*; D029589:Seminal Plasma Proteins / Q000302:isolation & purification / Q000378:metabolism*; D029607:Seminal Vesicle Secretory Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D020539:Sequence Analysis, Protein; D013094:Spermatozoa / Q000187:drug effects; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism
D003911:Dextrans; D008351:Mannans; D029589:Seminal Plasma Proteins; D029607:Seminal Vesicle Secretory Proteins; C013241:ribonuclease dimer; C424303:seminal vesicle secretory protein 109, Bos taurus; D019716:Tissue Inhibitor of Metalloproteinase-2; D005632:Fructose; D006493:Heparin; D006003:Glycogen; D007444:Inulin; D012260:Ribonucleases; D005947:Glucose; D005690:Galactose