Endogenous and exogenous ARC in serum withdrawal mediated PC12 cell apoptosis: a new pro-apoptotic role for ARC.
The role of ARC (Apoptosis Repressor with Caspase Recruitment Domain) in PC12 cell serum withdrawal driven apoptosis was studied. A progressive and massive increase in ARC protein occurs during serum withdrawal that correlates with declining survival and processing of caspase-2, previously shown to associate with ARC. This accumulation of ARC occurs in a transcriptional and translational independent manner. Additionally, ARC is localized exclusively in the nucleus of PC12 cells. Furthermore, transfection of PC12 cells with hARC-Flag promotes death and fails to protect the cells from apoptosis by serum withdrawal. Therefore, ARC functions in a pro-apoptotic manner in PC12 cell serum withdrawal induced apoptosis.
Dowds
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Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, New York 10595, USA.
Sabban
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D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D051017:Apoptosis Regulatory Proteins; D015152:Blotting, Northern; D053143:Caspase 2; D020169:Caspases / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D016895:Culture Media, Serum-Free; D015151:Immunoblotting; D008856:Microscopy, Fluorescence; D009124:Muscle Proteins / Q000235:genetics / Q000378:metabolism*; D020932:Nerve Growth Factor / Q000494:pharmacology; D016716:PC12 Cells; D014176:Protein Biosynthesis; D011499:Protein Processing, Post-Translational / Q000187:drug effects; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D014158:Transcription, Genetic; D014162:Transfection
D051017:Apoptosis Regulatory Proteins; D016895:Culture Media, Serum-Free; D009124:Muscle Proteins; C491788:Nol3 protein, rat; D012333:RNA, Messenger; D020932:Nerve Growth Factor; D053143:Caspase 2; D020169:Caspases