PI 3-K and T-cell activation: limitations of T-leukemic cell lines as signaling models.
Phosphoinositide 3-kinases (PI 3-Ks) phosphorylate the d3-hydroxyl position of phosphatidylinositol (4,5)-bisphosphate [PtdIns(4,5)P(2)] resulting in the generation of the 3'-phosphoinositide lipid PtdIns(3,4,5)P(3). PI 3-Ks are activated by a diverse set of receptors that play a role in determining T-cell function. It now seems that leukemic T cells, which are widely used as models for T-cell biology, show constitutive activation of PI 3-K-mediated signal-transduction pathways. Hence, studies of the role of PI 3-K in T-cell biology using leukemic cell lines might have misinterpreted the importance of this pathway for T-cell signal transduction.
Astoul
E
E
Lymphocyte Activation Laboratory, Imperial Cancer Research Fund, Lincolns Inn Fields, London, UK WC2A 3PX.
Edmunds
C
C
Cantrell
D A
DA
Ward
S G
SG
D004789:Enzyme Activation / Q000276:immunology; D006801:Humans; D019169:Jurkat Cells; D015458:Leukemia, T-Cell / Q000276:immunology* / Q000473:pathology; D008213:Lymphocyte Activation / Q000276:immunology; D019869:Phosphatidylinositol 3-Kinases / Q000276:immunology*; D015398:Signal Transduction / Q000276:immunology*; D014407:Tumor Cells, Cultured
D019869:Phosphatidylinositol 3-Kinases