title: Expression, cellular distribution and protein binding of the glioma amplified sequence (GAS41), a highly conserved putative transcription factor.
abstract: The glioma amplified sequence 41 (GAS41) was previously isolated by microdissection mediated cDNA capture from the glioblastoma multiforme cell line TX3868 and shown to be frequently amplified in human gliomas. We determined the complete cDNA sequence of the GAS41 gene, demonstrated that the GAS41 protein is evolutionarily conserved, specifically at the N-terminus, and identified the yeast transcription factor tf2f domain within the GAS41 sequence. A human multiple-tissue Northern blot revealed ubiquitous expression of GAS41 with the highest expression in human brain. After generating polyclonal antibodies we found GAS41 protein expression in the nucleus of the TX3868 cell line by Western blot analysis and immunofluorescence microscopy. The nuclear localization was confirmed for several human tumors including gliomas of different grades of malignancy. In neuroblastoma however, GAS41 was found in the nucleoli but not in the nucleoplasm. Yeast two-hybrid screening of the TX3868 cell line identified the nuclear mitotic apparatus protein (NuMA), the KIAA1009 protein, and prefoldin subunit 1 (PFDN1) as potential interacting partners of GAS41. We generated a polyclonal antibody against the KIAA1009 protein and we demonstrated that the KIAA1009 protein is a nuclear protein, which appears to be co-localized with the GAS41 protein and NuMA.
authors.affiliation: Institut für Humangenetik, Universitätskliniken des Saarlandes, 66421 Homburg/Saar, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Munnia
authors.forename: N
authors.initials: N
authors.lastname: Schütz
authors.forename: B F
authors.initials: BF
authors.lastname: Romeike
authors.forename: E
authors.initials: E
authors.lastname: Maldener
authors.forename: B
authors.initials: B
authors.lastname: Glass
authors.forename: R
authors.initials: R
authors.lastname: Maas
authors.forename: W
authors.initials: W
authors.lastname: Nastainczyk
authors.forename: W
authors.initials: W
authors.lastname: Feiden
authors.forename: U
authors.initials: U
authors.lastname: Fischer
authors.forename: E
authors.initials: E
authors.lastname: Meese
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary / Q000737:chemistry; D006801:Humans; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D011485:Protein Binding; D014157:Transcription Factors / Q000032:analysis* / Q000737:chemistry / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D014157:Transcription Factors; C109446:YEATS4 protein, human