Large-scale, multicenter study of cerebrospinal fluid tau protein phosphorylated at serine 199 for the antemortem diagnosis of Alzheimer's disease.
We surveyed a total of 570 cerebrospinal fluid (CSF) samples from a variety of diseases, including Alzheimer's disease (AD; n = 236), non-AD-demented and nondemented diseases (n = 239), and normal controls (n = 95) to quantitate levels of tau protein phosphorylated at serine 199 (CSF/phospho-tau199) by a recently established sandwich ELISA. The CSF/phospho-tau199 levels in the AD group were significantly elevated compared to those in all the other non-AD groups. Receiver operating characteristics curves showed that the diagnostic sensitivity and specificity for the AD group vs all the other non-AD groups using the CSF/phospho-tau199 were 85.2% and 85.0%, respectively. Furthermore, there was a significant positive correlation between CSF/phospho-tau199 and CSF/total-tau levels in the AD group. Elevated CSF/phospho-tau199 in the AD group was noted irrespective of age, gender, dementia severity, and number of apolipoprotein E4 alleles. Thus, we suggest that CSF/phospho-tau199 may be a novel and logical biomarker in supporting antemortem diagnosis of AD.
Itoh
N
N
Department of Neurology, Mie University School of Medicine, Tsu, Japan.
Arai
H
H
Urakami
K
K
Ishiguro
K
K
Ohno
H
H
Hampel
H
H
Buerger
K
K
Wiltfang
J
J
Otto
M
M
Kretzschmar
H
H
Moeller
H J
HJ
Imagawa
M
M
Kohno
H
H
Nakashima
K
K
Kuzuhara
S
S
Sasaki
H
H
Imahori
K
K
D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000134:cerebrospinal fluid* / Q000235:genetics*; D053327:Apolipoprotein E4; D001057:Apolipoproteins E / Q000235:genetics*; D015415:Biomarkers / Q000134:cerebrospinal fluid*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D010766:Phosphorylation; D012372:ROC Curve; D015203:Reproducibility of Results; D012694:Serine / Q000235:genetics*; D012737:Sex Factors; D016875:tau Proteins / Q000134:cerebrospinal fluid*
D053327:Apolipoprotein E4; D001057:Apolipoproteins E; D015415:Biomarkers; D016875:tau Proteins; D012694:Serine