DA rat NK(+)CD3(-) cells inhibit autoreactive T-cell responses.
We isolated natural killer (NK) cells from DA rat bone marrow (BM) by staining with PE anti-NKR-P1A and FACS sorting (>98% NK(+)). The purified NK cells inhibit T-cell proliferation in a dosage-dependent fashion and suppressed production of the proinflammatory Th1 cytokine, IFN-gamma. When activated in vitro with Con A supernatant (CAS), the purified NK cells secrete the beta-chemokine monocyte chemoattractant protein-1 (MCP-1) and upregulate MCP-1 mRNA. The activated NK cells also express IFN-gamma mRNA. Sorted NK(+)CD3(-) cells, from which NKT cells have been excluded, also inhibit autoreactive T-cell responses to myelin basic protein (MBP). These findings are consistent with a role for conventional NK cells in maintaining immune homeostasis, by eliminating autoreactive T cells that have inadvertently become activated.
Wolf
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NA
Department of Immunology and Microbiology, Wayne State University School of Medicine, 540 East Canfield Avenue, Detroit, MI 48201, USA.
Swanborg
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RH
D000818:Animals; D001854:Bone Marrow Cells / Q000276:immunology / Q000502:physiology; D017252:CD3 Complex / Q000032:analysis*; D002455:Cell Division / Q000502:physiology; D002469:Cell Separation; D018932:Chemokine CCL2 / Q000235:genetics / Q000378:metabolism; D005434:Flow Cytometry; D007371:Interferon-gamma / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000235:genetics; D007694:Killer Cells, Natural / Q000276:immunology* / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013601:T-Lymphocytes / Q000166:cytology* / Q000276:immunology*
D017252:CD3 Complex; D018932:Chemokine CCL2; D012333:RNA, Messenger; D007371:Interferon-gamma