Preparation of monospecific polyclonal antibodies against Sambucus nigra lectin related protein, a glycosylated plant protein.
A simple, but highly efficient, method was developed for the purification of monospecific antibodies against the plant glycoprotein Sambucus nigra lectin related protein. In a first step, the antiserum is purified by affinity chromatography on a column with the immobilized antigen. To deplete the affinity-purified antiserum from aspecific cross-reacting antibodies directed against the glycan part of the glycoprotein, a second affinity chromatography on an unrelated plant glycoprotein, in casu the Robinia pseudoacacia agglutinin, is included.
Desmyter
S
S
Laboratory for Phytopathology and Plant Protection, Katholieke Universiteit Leuven, Belgium.
Vandenbussche
F
F
Van Damme
E J
EJ
Peumans
W J
WJ
D000818:Animals; D000906:Antibodies / Q000276:immunology* / Q000302:isolation & purification*; D000918:Antibody Specificity; D000941:Antigens / Q000276:immunology; D015153:Blotting, Western; D002846:Chromatography, Affinity; D006023:Glycoproteins / Q000276:immunology; D006031:Glycosylation; D037102:Lectins / Q000276:immunology*; D008297:Male; D037121:Plant Lectins; D010940:Plant Proteins / Q000276:immunology*; D011817:Rabbits; D027924:Sambucus nigra / Q000276:immunology*
D000906:Antibodies; D000941:Antigens; D006023:Glycoproteins; D037102:Lectins; D037121:Plant Lectins; D010940:Plant Proteins