title: Impact of current cryopreservation procedures on mechanical and functional properties of human aortic homografts.
abstract: We evaluated the impact f standard cryopreservation on mechanical and functional properties of human aortic homografts. From 14 human heart-valve donors, the thoracic descending aorta was obtained. Effects of cryopreservation on mechanical (elastic properties and breaking stress) and smooth muscle cell (SMC) and endothelium function were tested. Cryopreservation (cryo) did not significantly affect Young's modulus of elastin (fresh: 3.1 +/- 1.0, cryo: 2.7 +/- 0.9 x 10(5) Nm(-2)), collagen recruitment pressure (fresh: 1.1 +/- 0.3, cryo: 1.1 +/- 0.4 x 10(4) Nm(-2)), distensibility (fresh: 3.8 +/- 1.8, cryo: 3.6 +/- 1.6 x 10(5) N(-1)m2), or breaking stress (fresh: 2.4 +/- 1.0, cryo: 2.2 +/- 1.0 x 10(6) Nm(-2)). Following explantation, no endothelium-dependent relaxation was found. SMC function and endothelium-independent relaxation were mainly intact after explantation but significantly decreased after cryopreservation. Aortic mechanical properties are not influenced by cryopreservation. Following explantation, almost no endothelial cell function is present, and SMC contractility is strongly affected after cryopreservation.
authors.affiliation: Bio Implant Services Foundation, Plesmanlaan 100, 2301 CH, Leiden, The Netherlands. S. E. Langerak@lumc.nl
authors.forename: S E
authors.initials: SE
authors.lastname: Langerak
authors.forename: M
authors.initials: M
authors.lastname: Groenink
authors.forename: E E
authors.initials: EE
authors.lastname: van der Wall
authors.forename: C
authors.initials: C
authors.lastname: Wassenaar
authors.forename: E
authors.initials: E
authors.lastname: Vanbavel
authors.forename: M C
authors.initials: MC
authors.lastname: van Baal
authors.forename: J A
authors.initials: JA
authors.lastname: Spaan
mesh_terms: D000293:Adolescent; D000328:Adult; D001011:Aorta / Q000502:physiology / Q000637:transplantation*; D015925:Cryopreservation; D004730:Endothelium, Vascular / Q000502:physiology; D006801:Humans; D008875:Middle Aged; D009131:Muscle, Smooth, Vascular / Q000502:physiology; D014184:Transplantation, Homologous; D014661:Vasoconstriction / Q000187:drug effects