[Mutations in the Escherichia coli 23S rRNA increase the rate of peptidyl-tRNA dissociation from the ribosome].
We have studied in vivo the phenotypes of 23S rRNA mutations G2582A, G2582U, G2583C, and U2584C, which are located at the A site of Escherichia coli 50S ribosomal subunit. All mutant rRNAs incorporated into 50S ribosomal subunits. Upon sucrose gradient fraction of cell lysates, 23S rRNAs mutated at G2582 to A and G2583 to C accumulated in the 50S and 70S fractions and were under-represented in the polysome fraction. Induction of 23S rRNAs mutated at G2582 and G2583 lead to a drastic reduction in cell growth. In addition, mutations G2582A and G2583C reduced to one-third the total protein synthesis but not the RNA synthesis. Finally, we show that 23S rRNA mutations G2582A, G2582U, and G2583C cause a significant increase in peptidyl-tRNA drop-off from ribosomes, thereby reducing translational processivity. The results clearly show that tRNA-23S rRNA interaction has an essential role in maintaining the processivity of translation.
Maĭvali
U
U
Institute of Molecular and Cell Biology, Tartu University, Tartu, 51010, Estonia.
Saarma
U
U
Remme
Ia
Ia
D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D009154:Mutation; D014176:Protein Biosynthesis; D012338:RNA, Ribosomal, 23S / Q000235:genetics* / Q000378:metabolism; D012346:RNA, Transfer, Amino Acyl / Q000235:genetics* / Q000378:metabolism; D012270:Ribosomes / Q000235:genetics* / Q000378:metabolism
D012338:RNA, Ribosomal, 23S; D012346:RNA, Transfer, Amino Acyl; C019037:tRNA, peptidyl-
Mutatsii v 23S ribosomnoĭ RNK Escherichia coli uvelichivaiut skorost' dissotsiatsii peptidil-tRNK iz kompleksa s ribosomami.