Internalization-defective mutants of somatostatin receptor subtype 2 exert normal signaling functions in hematopoietic cells.
The regulatory peptide somatostatin (SST) acts via a family of G-protein-coupled receptors comprising five subtypes (SSTR1-5). G-protein-coupled receptors activate multiple signaling mechanisms, which variably depend on internalization and intracellular routing of activated receptors. We have recently demonstrated that hematopoietic precursors express SSTR2 and that SST is a chemoattractant for these cells. Herein, we characterize critical regions in SSTR2 involved in endocytosis and describe how ligand-induced internalization impacts on two major signaling functions of SSTR2 in hematopoietic cells, the activation of the Erk pathway and the induction of promigratory responses.
Oomen
S P
SP
Institute of Hematology, Erasmus University Rotterdam, The Netherlands.
Hofland
L J
LJ
Lamberts
S W
SW
Löwenberg
B
B
Touw
I P
IP
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002633:Chemotaxis / Q000187:drug effects; D017931:DNA Primers / Q000235:genetics; D019204:GTP-Binding Proteins / Q000378:metabolism; D006412:Hematopoietic Stem Cells / Q000187:drug effects / Q000502:physiology; D006801:Humans; D066298:In Vitro Techniques; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D017481:Receptors, Somatostatin / Q000235:genetics* / Q000502:physiology*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D013004:Somatostatin / Q000494:pharmacology
D017931:DNA Primers; D017481:Receptors, Somatostatin; D011994:Recombinant Proteins; D013004:Somatostatin; C093733:somatostatin receptor 2; D019204:GTP-Binding Proteins