Detection of bacteraemia in critically ill patients using 16S rDNA polymerase chain reaction and DNA sequencing.
OBJECTIVE
To confirm the sensitivity of the polymerase chain reaction (PCR) technique (versus blood cultures) and to gain a better understanding of the incidence of true- and false-positive results when using this technique.


METHODS
Observational study.


METHODS
Fourteen-bed, level 3 intensive care unit.


METHODS
Hundred twenty-six critically ill adult patients. Hundred ninety-seven blood culture and PCR samples taken as clinically indicated for suspected sepsis, according to routine ICU protocol.


RESULTS
The PCR product (16SrDNA: 341F-1195R) was sequenced and compared with a database of known species (Genebank) to identify the bacterial nucleic acid. The PCR or blood culture result was classified as a true-positive if there was other microbiological or clinical supporting evidence.
Sleigh
J
J
Department of Intensive Care, Waikato Hospital, Pembroke Street, Hamilton, New Zealand. sleighj@hwl.co.nz
Cursons
R
R
La Pine
M
M
D000328:Adult; D016470:Bacteremia / Q000175:diagnosis*; D015373:Bacterial Typing Techniques; D018929:Cell Culture Techniques; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D005189:False Positive Reactions; D015723:Gene Library; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA / Q000379:methods*; D018709:Statistics, Nonparametric
D004269:DNA, Bacterial; D004275:DNA, Ribosomal