Continuous superporous agarose beds in radial flow columns.
Continuous superporous agarose beds constitute a new support material for chromatography, biocatalysis and electrophoresis. The bed consists of a single piece of agarose gel, homogeneously transected by flow-carrying pores, which easily can be varied in the range of 10-100 microm. In this work, large diameter beds (60 mm) were prepared and used in specially designed radial flow columns. The basic chromatographic properties of the beds were investigated by size-exclusion chromatography experiments. In an affinity chromatography application one bed was derivatized with Cibacron Blue 3GA and used for the purification of lactate dehydrogenase from a crude bovine heart extract. In a biotransformation application one bed was provided with immobilized beta-galactosidase and used in the production of lactose-free milk.
Gustavsson
P E
PE
Department of Pure and Applied Biochemistry, Center for Chemistry and Chemical Engineering, Lund University, Sweden. per-erik.gustavsson@tbiokem.lth.se
Larsson
P O
PO
D000818:Animals; D002417:Cattle; D002853:Chromatography, Liquid / Q000295:instrumentation*; D004800:Enzymes, Immobilized / Q000737:chemistry; D007770:L-Lactate Dehydrogenase / Q000302:isolation & purification; D008892:Milk; D012685:Sepharose / Q000737:chemistry*; D001616:beta-Galactosidase / Q000737:chemistry
D004800:Enzymes, Immobilized; D012685:Sepharose; D007770:L-Lactate Dehydrogenase; D001616:beta-Galactosidase