title: Determination of 4,4'-dinitrocarbanilide (DNC), the active component of the antifertility agent nicarbazin, in chicken, duck, and goose plasma.
abstract: 4,4'-Dinitrocarbanilide (DNC) was extracted from chicken, duck, and goose plasma and isolated by reversed-phase high-performance liquid chromatography. DNC was detected by ultraviolet absorbance at 347 nm and quantified by comparison to a calibration standard. Recovery data were determined by analyzing DNC-fortified control plasma. The mean recovery of DNC in fortified chicken plasma samples was 99.7 +/- 1.9% for 0.18 and 9.1 ppm DNC, and in fortified duck and goose plasma samples was 99.5 +/- 4.9% and 101.4 +/- 4.5%, respectively, for 0.18, 9.1, and 18 ppm DNC.
authors.affiliation: Analytical Chemistry Project, U.S. Department of Agriculture/Animal and Plant Health Inspection Service/National Wildlife Research Center, 4101 LaPorte Avenue, Fort Collins, Denver, Colorado 80521, USA. thomas.m.primus@usda.gov
authors.forename: T M
authors.initials: TM
authors.lastname: Primus
authors.forename: D J
authors.initials: DJ
authors.lastname: Kohler
authors.forename: M A
authors.initials: MA
authors.lastname: Goodall
authors.forename: C
authors.initials: C
authors.lastname: Yoder
authors.forename: D
authors.initials: D
authors.lastname: Griffin
authors.forename: L
authors.initials: L
authors.lastname: Miller
authors.forename: J J
authors.initials: JJ
authors.lastname: Johnston
mesh_terms: D000818:Animals; D002138:Calibration; D002645:Chickens; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004372:Ducks; D005298:Fertility / Q000187:drug effects*; D005777:Geese; D009528:Nicarbazin / Q000097:blood* / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
chemical_list: D009528:Nicarbazin