title: Glyceraldehyde-3-phosphate dehydrogenase is negatively regulated by ADP-ribosylation in the fungus Phycomyces blakesleeanus.
abstract: Dormant spores of Phycomyces blakesleeanus contain a 37 kDa protein that is endogenously mono-ADP-ribosylated. This protein was purified and identified as glyceraldehyde-3-phosphate dehydrogenase (GAPDH) by N-terminal sequencing and homology analysis. GAPDH enzymic activity changed dramatically upon spore germination, being maximal at stages where ADP-ribosylation was nearly undetectable. The presence of glyceraldehyde 3-phosphate in this reaction affected the [(32)P]ADP-ribosylation of the GAPDH. ADP-ribosylation of the GAPDH occurred by transfer of the ADP-ribose moiety from NAD to an arginine residue. A model for the regulation of GAPDH activity and its role in spore germination in P. blakesleeanus is proposed.
authors.affiliation: Instituto de Investigación en Biologı́a Experimental, Facultad de Quı́mica, Universidad de Guanajuato, Apdo. postal 187, Guanajuato, Gto, 36000, Mexico1.
authors.forename: Martha
authors.initials: M
authors.lastname: Deveze-Alvarez
authors.affiliation: Instituto de Investigación en Biologı́a Experimental, Facultad de Quı́mica, Universidad de Guanajuato, Apdo. postal 187, Guanajuato, Gto, 36000, Mexico1.
authors.forename: Jesús
authors.initials: J
authors.lastname: Garcı A-Soto
authors.affiliation: Instituto de Investigación en Biologı́a Experimental, Facultad de Quı́mica, Universidad de Guanajuato, Apdo. postal 187, Guanajuato, Gto, 36000, Mexico1.
authors.forename: Guadalupe
authors.initials: G
authors.lastname: Martı Nez-Cadena
mesh_terms: D000246:Adenosine Diphosphate Ribose / Q000378:metabolism*; D001120:Arginine / Q000737:chemistry; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000737:chemistry / Q000378:metabolism*; D008954:Models, Biological; D008970:Molecular Weight; D009243:NAD / Q000378:metabolism; D010800:Phycomyces / Q000201:enzymology* / Q000502:physiology; D011499:Protein Processing, Post-Translational; D013172:Spores, Fungal / Q000201:enzymology
chemical_list: D009243:NAD; D000246:Adenosine Diphosphate Ribose; D001120:Arginine; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases