Effect of the herbicide 4-CPA on human erythrocyte antioxidant enzymes in vitro.
To investigate the possible role of oxygen free radicals and oxidant stress in the toxic effects of phenoxyherbicides, we studied the in vitro effect of 4-chlorophenoxyacetic acid (4-CPA) on various human erythrocyte antioxidant enzymes, namely glucose-6-phosphate dehydrogenase, catalase, selenium-dependent glutathione peroxidase, glutathione reductase and Cu/Zn-superoxide dismutase. 4-CPA added in a dose of 1 ppm to human erythrocytes for 1 h caused a significant reduction in glucose-6-phosphate dehydrogenase (P <0.001) and catalase (P <0.001) activities, but did not significantly affect the activities of other enzymes. Such selective inactivation of specific erythrocyte antioxidant enzymes may play a role in the toxic effects of phenoxyherbicides.
Alicigüzel
Y
Y
Department of Biochemistry, Faculty of Medicine, Akdeniz University, Arapsuyu, Antalya, Turkey. yakup@hipokrat.med.akdeniz.edu.tr
Ozdem
S
S
Demir
A Y
AY
Unal
F
F
Kumbul
D
D
Ozdem
S S
SS
Perry
G
G
Smith
M A
MA
D015084:2,4-Dichlorophenoxyacetic Acid / Q000031:analogs & derivatives / Q000494:pharmacology*; D000975:Antioxidants / Q000032:analysis*; D002374:Catalase / Q000037:antagonists & inhibitors* / Q000097:blood; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D004912:Erythrocytes / Q000187:drug effects* / Q000201:enzymology; D005609:Free Radicals; D005954:Glucosephosphate Dehydrogenase / Q000037:antagonists & inhibitors* / Q000097:blood; D005979:Glutathione Peroxidase / Q000037:antagonists & inhibitors* / Q000097:blood; D005980:Glutathione Reductase / Q000037:antagonists & inhibitors* / Q000097:blood; D006540:Herbicides / Q000494:pharmacology*; D006801:Humans; D018384:Oxidative Stress; D012643:Selenium; D013482:Superoxide Dismutase / Q000037:antagonists & inhibitors* / Q000097:blood
D000975:Antioxidants; D004791:Enzyme Inhibitors; D005609:Free Radicals; D006540:Herbicides; D015084:2,4-Dichlorophenoxyacetic Acid; C007017:4-chlorophenoxyacetic acid; D005954:Glucosephosphate Dehydrogenase; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase; D012643:Selenium