Ethyl acetate extract of the stem bark of Cudrania tricuspidata induces apoptosis in human leukemia HL-60 cells.
Apoptosis is now widely accepted as playing a role in tumorigenesis. An effective compound which can kill tumors via apoptotic pathway appears to be a relevant strategy to suppress various human tumors. The ethyl acetate extract from the stem bark of Cudrania tricuspidata (EACT) showed dose- and time-dependent cytotoxic effects on human leukemia HL-60 cells. DNA fragmentation and morphological changes, accompanied by condensed and fragmented nuclei, were observed in the cells cultured for 6 hr with EACT. These results suggest that the cytotoxicity of the crude extract from Cudrania tricuspidata against HL-60 cells is due to apoptosis.
Seo
W G
WG
Department of Microbiology and Immunology, Wonkwang University School of Medicine, Iksan, Chonbuk, South Korea.
Pae
H O
HO
Oh
G S
GS
Chai
K Y
KY
Yun
Y G
YG
Chung
H T
HT
Jang
K K
KK
Kwon
T O
TO
D000085:Acetates / Q000494:pharmacology; D000704:Analysis of Variance; D000972:Antineoplastic Agents, Phytogenic / Q000037:antagonists & inhibitors*; D017209:Apoptosis / Q000187:drug effects*; D004273:DNA, Neoplasm / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000494:pharmacology* / Q000627:therapeutic use; D004587:Electrophoresis, Agar Gel; D018922:HL-60 Cells / Q000187:drug effects*; D006801:Humans; D066298:In Vitro Techniques; D024301:Plant Bark; D013997:Time Factors; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000085:Acetates; D000972:Antineoplastic Agents, Phytogenic; D004273:DNA, Neoplasm; D004365:Drugs, Chinese Herbal; C007650:ethyl acetate