Perineurial cell differentiation in neurofibromas. Report of eight cases including a case with composite perineurioma-neurofibroma features.
Among 99 cases of neurofibroma (NF), eight tumors (8%) contained epithelial membrane antigen (EMA)-positive perineurial cells inside the lesions. These cells were numerous and represented a significant part of the tumor cell population. In case 7, EMA-positive cells represented approximately half of the tumor. These patients' ages ranged from 23 to 73 years (average 51 years). Six patients were females and two were males. Neurofibromatosis type 1 was present in one case. The histological types of neurofibromas with EMA-positive cells were as follows: cutaneous, well-circumscribed, localized type in four cases; cutaneous diffuse type in one case; subcutaneous, well-circumscribed type in two cases; and subcutaneous plexiform type in one case. Perineurial cell differentiation was suspected by examining routinely stained sections in four cases; it was unsuspected in four cases. The perineurial cells were arranged in a pattern similar to that of "pure" perineurioma. They possessed thin bipolar processes and were arranged in laminar fascicles and whorls. In cellular areas, the cytoplasm was more eosinophilic and cell borders were poorly visible. In contrast, when the stroma was myxoid, it accentuated the shape of individual cells. As these morphological features of perineurial cell differentiation in NF are not entirely specific, EMA staining is recommended to prove the perineurial cell differentiation in neurofibromas. An additional immunohistochemical result of this study is the presence of numerous CD34-positive cells in all neurofibromas, which is similar to previous studies.
Zamecnik
M
M
Department of Pathology, Slovak Postgraduate Academy of Medicine, Derer's Faculty Hospital, Bratislava, Slovak Republic.
Michal
M
M
D000328:Adult; D000368:Aged; D018952:Antigens, CD34 / Q000032:analysis; D014408:Biomarkers, Tumor / Q000032:analysis; D002454:Cell Differentiation; D002471:Cell Transformation, Neoplastic / Q000737:chemistry / Q000473:pathology*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D018396:Mucin-1 / Q000032:analysis; D009455:Neurofibroma / Q000737:chemistry / Q000473:pathology*; D010525:Peripheral Nerves / Q000737:chemistry / Q000473:pathology*; D012189:Retrospective Studies; D009418:S100 Proteins / Q000032:analysis; D012878:Skin Neoplasms / Q000737:chemistry / Q000473:pathology*
D018952:Antigens, CD34; D014408:Biomarkers, Tumor; D018396:Mucin-1; D009418:S100 Proteins