Butyrate and propionate downregulate ERK phosphorylation in HT-29 colon carcinoma cells prior to differentiation.
We have characterized the effects of different short-chain fatty acids (SCFAs) on cell growth and differentiation as well as the phosphorylation state of ERK1 and 2 in the human colon adenocarcinoma cell line HT-29. Of the five SCFAs tested, only butyrate and propionate impaired cellular proliferation. Moreover, butyrate and propionate specifically resulted in a decrease in ERK1 and 2 phosphorylation at 3 and 6 hours post-treatment, suggesting a correlation between the ability of these SCFAs to inhibit cellular proliferation and decrease ERK phosphorylation. Notably, the decrease in ERK phosphorylation was observed prior to the induction of the differentiation markers alkaline phosphatase (AP) and carcinoembryonic antigen (CEA) by butyrate and propionate from days 6 to 18 post-treatment. In the case of butyrate- and propionate-induced differentiation, ERK phosphorylation is a marker and may play a role in the proliferation and/or differentiation states of this cell line.
Davido
D J
DJ
Medizinische Klinik, Universität Würzburg, Josef Schneider Str. 2, 97080 Würzburg, Germany.
Richter
F
F
Boxberger
F
F
Stahl
A
A
Menzel
T
T
Lührs
H
H
Löffler
S
S
Dusel
G
G
Rapp
U R
UR
Scheppach
W
W
D000469:Alkaline Phosphatase / Q000032:analysis / Q000378:metabolism; D002087:Butyrates / Q000494:pharmacology*; D002272:Carcinoembryonic Antigen / Q000032:analysis / Q000378:metabolism; D002454:Cell Differentiation; D015536:Down-Regulation; D019073:HT29 Cells / Q000201:enzymology*; D006801:Humans; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism*; D048052:Mitogen-Activated Protein Kinase 3; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D010766:Phosphorylation; D011422:Propionates / Q000494:pharmacology*; D015398:Signal Transduction
D002087:Butyrates; D002272:Carcinoembryonic Antigen; D011422:Propionates; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; D020928:Mitogen-Activated Protein Kinases; D000469:Alkaline Phosphatase