Selective transcription and modulation of resting T cell activity by preintegrated HIV DNA.
The quiescent nature of most peripheral T cells poses an effective limitation to human immunodeficiency virus (HIV) replication and, in particular, to viral integration into the host chromatin. Two HIV proteins, Nef and Tat, increase T cell activity, but a requirement of integration for viral gene expression would preclude a role for these proteins in resting cells. Here, we report that HIV infection leads to selective transcription of the nef and tat genes before integration. This preintegration transcription in quiescent cells leads to increased T cell activation and viral replication.
Wu
Y
Y
Laboratory of Molecular Biology, National Institute of Mental Health, Bethesda, MD 20892-4034, USA.
Marsh
J W
JW
D019380:Anti-HIV Agents / Q000494:pharmacology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000502:physiology / Q000821:virology*; D004279:DNA, Viral / Q000235:genetics* / Q000378:metabolism; D015693:Gene Products, nef / Q000378:metabolism; D015696:Gene Products, tat / Q000378:metabolism; D015754:Genes, nef; D015756:Genes, tat; D019427:HIV Integrase / Q000235:genetics / Q000378:metabolism; D015497:HIV-1 / Q000235:genetics* / Q000502:physiology; D006801:Humans; D007376:Interleukin-2 / Q000378:metabolism; D008213:Lymphocyte Activation; D009154:Mutation; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic; D016662:Virus Integration; D014779:Virus Replication; D015215:Zidovudine / Q000494:pharmacology; D054311:nef Gene Products, Human Immunodeficiency Virus; D054322:tat Gene Products, Human Immunodeficiency Virus
D019380:Anti-HIV Agents; D004279:DNA, Viral; D015693:Gene Products, nef; D015696:Gene Products, tat; D007376:Interleukin-2; D054311:nef Gene Products, Human Immunodeficiency Virus; D054322:tat Gene Products, Human Immunodeficiency Virus; D015215:Zidovudine; D019427:HIV Integrase