title: Genetic analysis of hepatitis A virus outbreak in France confirms the co-circulation of subgenotypes Ia, Ib and reveals a new genetic lineage.
abstract: Genetic analysis of selected genome regions of hepatitis A Virus (HAV) suggested that distinct genotype could be defined in different geographic locations. In order to study the degree of genetic variability among HAV isolated during a single epidemic outbreak, sequences from a 148 base pair segment within the VP1 amino terminal region were obtained for eight distinct HAV isolates from an outbreak that occurred in North Bretagne (France). These sequences were compared among themselves and with published sequences from 30 different strains that represented different HAV sub-genotypes that were isolated all over the world. Phylogenetic analysis revealed an extensive genetic heterogeneity among strains belonging to the same outbreak and revealed co-circulation of sub-genotype IA, IB, and the presence of IIIA sub-genotype for the first time in a Mediterranean country.
authors.affiliation: Laboratorie de Virologie UPRES-EA1156, Institut de Biologie, Centre Hospitalier Regional Universitaire de Nantes, Nantes, France.
authors.forename: M
authors.initials: M
authors.lastname: Costa-Mattioli
authors.forename: S
authors.initials: S
authors.lastname: Monpoeho
authors.forename: C
authors.initials: C
authors.lastname: Schvoerer
authors.forename: B
authors.initials: B
authors.lastname: Besse
authors.forename: M H
authors.initials: MH
authors.lastname: Aleman
authors.forename: S
authors.initials: S
authors.lastname: Billaudel
authors.forename: J
authors.initials: J
authors.lastname: Cristina
authors.forename: V
authors.initials: V
authors.lastname: Ferré
mesh_terms: D000328:Adult; D001483:Base Sequence; D003001:Cloning, Molecular; D004196:Disease Outbreaks; D005260:Female; D005602:France / Q000453:epidemiology; D014644:Genetic Variation; D005838:Genotype; D006506:Hepatitis A / Q000453:epidemiology*; D006507:Hepatovirus / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein; D015678:Viral Structural Proteins / Q000235:genetics
chemical_list: D012367:RNA, Viral; C096539:VP1 protein, hepatitis A virus; D015678:Viral Structural Proteins