Structural and serological characterisation of the O-antigenic polysaccharide of the lipopolysaccharide from Acinetobacter strain 96 (DNA group 11).
A polysaccharide containing D-Manp, L-Fucp (6-deoxygalactopyranose, fucose) and D-GlcpNAc was isolated by mild acid hydrolysis, followed by gel-permeation chromatography, from the lipopolysaccharide derived from Acinetobacter strain 96 (DNA group 11). The structure of the O-antigen was determined by compositional analysis and NMR spectroscopy of the polysaccharide as: [carbohydrate structure see text] A monoclonal antibody obtained after immunization of mice with heat-killed bacteria of Acinetobacter strain 96 was shown to bind to the O-antigen and did not cross-react with any Acinetobacter O-antigen of known structure.
Vinogradov
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EV
Institute for Biological Sciences, NRCC, Ottawa, Ontario, Canada.
Pantophlet
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R
Brade
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Holst
O
O
D000150:Acinetobacter / Q000737:chemistry* / Q000145:classification; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology; D015153:Blotting, Western; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D003429:Cross Reactions; D000939:Epitopes; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry*; D012703:Serotyping
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000939:Epitopes; D019081:O Antigens