title: Involvement of sigma(S) in starvation-induced transposition of Pseudomonas putida transposon Tn4652.
abstract: Transpositional activity of mobile elements can be induced by different environmental stresses. Here, we present evidence that transposition of Tn4652 is elevated in stationary-phase Pseudomonas putida and suppressed in an isogenic sigma(S)-defective strain. We demonstrate that transcription from the Tn4652 transposase promoter is controlled by the stationary-phase-specific sigma factor sigma(S). To our knowledge, this is the first example of direct stationary-phase-specific regulation of a mobile element transposase. Data presented in this report support the idea that activation of transposition under stressful conditions could be an inducible process.
authors.affiliation: Estonian Biocentre and Institute of Molecular and Cell Biology, Tartu University, 51010 Tartu, Estonia.
authors.forename: H
authors.initials: H
authors.lastname: Ilves
authors.forename: R
authors.initials: R
authors.lastname: Hõrak
authors.forename: M
authors.initials: M
authors.lastname: Kivisaar
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D004251:DNA Transposable Elements; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D016958:Pseudomonas putida / Q000235:genetics* / Q000254:growth & development*; D012808:Sigma Factor / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic; D019895:Transposases / Q000235:genetics* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D012808:Sigma Factor; C122137:TnpC protein, Pseudomonas putida; D014157:Transcription Factors; C069567:sigma factor KatF protein, Bacteria; D019895:Transposases