Strains isogenic to S288C used in the yeast genome sequencing programme carry a functional KSS1 gene.
In Saccharomyces cerevisiae, the KSS1 gene encodes the MAP kinase of the invasive/filamentous growth pathway. In addition to its role in this signal transduction pathway, Kssl can replace the Fus3 MAP kinase in the pheromone-response pathway, in the absence of FUS3. Previous work indicated that derivatives of the S288C strain carry a mutant kss1 allele. Here, we report evidence that S288C derivatives used in the Yeast Genome Sequencing Programme carry a functional KSS1 gene and can thus be used to study the regulation of gene expression by KSS1.
Morillon
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UPR 9073 du CNRS, Institut de Biologie Physico-Chimique, Paris, France.
Springer
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Lesage
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D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D016681:Genome, Fungal; D005838:Genotype; D020928:Mitogen-Activated Protein Kinases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D010675:Pheromones / Q000502:physiology; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins
D005656:Fungal Proteins; D010675:Pheromones; D011994:Recombinant Proteins; D029701:Saccharomyces cerevisiae Proteins; C075580:FUS3 protein, S cerevisiae; C541984:KSS1 protein, S cerevisiae; D020928:Mitogen-Activated Protein Kinases