[IFN-r/IL-4 expression in peripheral blood mononuclear cells of the patients with chronic severe hepatitis].
OBJECTIVE
To understand the expressions of interferon-r (INF-r) and interleukin-4 (IL-4) in peripheral blood mononuclear cells (PBMCs) of the patients with chronic severe hepatitis (CSH).


METHODS
PBMCs from the patients with CSH were separated routinely and stimulated by PMA/Ionomycin/Monensin. The production of IFN-r/IL-4 by CD4+ T cells in PBMC was determined by fluorescence activated call sorting (FACS) analysis and fluorescence-quantitative PCR assay for quantification of HBV DNA.


RESULTS
The percentage of IFN-gamma-producing T cells in CD4+ T cells was higher in patients with CSH (7.2%-26.3%) than in normal controls (2.2%-11.9%) (P<0.01). The percentage of Th1 cells increased significantly with the raise of hepatic inflammation activity. The percentage of IL-4-producing T cells in CD4+ T cells did not differ significantly between patients with CSH and normal controls. The quantification of HBV DNA decreased significantly with the increase of the percentage of IFN-gamma-producing T cells.


CONCLUSIONS
Th1 cell is associated with hepatic inflammatory activity and IFN-gamma depresses HBV replication.
Dai
W
W
East Lake Hospital, Shenzhen 518020, China.
Yu
W
W
Jiang
R
R
Shu
D
D
Liu
Y
Y
Li
X
X
Zhang
P
P
D000328:Adult; D000368:Aged; D002908:Chronic Disease; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D006505:Hepatitis / Q000276:immunology*; D019694:Hepatitis B, Chronic / Q000276:immunology; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis*; D015847:Interleukin-4 / Q000096:biosynthesis*; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D018417:Th1 Cells / Q000502:physiology
D004279:DNA, Viral; D015847:Interleukin-4; D007371:Interferon-gamma