Long-lived polyclonal B-cell lines derived from midgestation mouse embryo lymphohematopoietic progenitors reconstitute adult immunodeficient mice.
Lymphohematopoietic progenitors derived from midgestation mouse embryos were established in long-term cultures with stromal cell monolayers and interleukin 7 (IL-7), giving rise to B-lineage cell lines. The initial emergence and in vitro establishment of these early embryo cell lines were highly sensitive to IL-7-mediated signals, in comparison to cell lines similarly obtained using precursors from late fetal liver (> 13 days postcoitum) and adult bone marrow. The early embryo-derived progenitors spontaneously differentiated in vitro to CD19(+)IgM(+) immature B cells in the presence of optimal concentrations of IL-7, in contrast to those progenitors obtained from late gestation and adult mice, whose differentiation only occurred in the absence of IL-7. The newly in vitro-generated B cells of the early embryo cell lines repopulated adult immunodeficient severe combined immunodeficient mice on their adoptive transfer in vivo and generated specific humoral immune responses after immunization.
Martínez-M
J A
JA
Centro Nacional de Biología Fundamental, Instituto de Salud Carlos III (ISCIII), Majadahonda 28220, Spain.
Minguet
S
S
Gonzalo
P
P
Soro
P G
PG
de Andrés
B
B
Izcue
A
A
Marcos
M A
MA
Gaspar
M L
ML
D019297:2,4-Dinitrophenol / Q000276:immunology; D019264:Adoptive Transfer; D000818:Animals; D001402:B-Lymphocytes / Q000637:transplantation*; D002454:Cell Differentiation; D002460:Cell Line; D002999:Clone Cells; D004622:Embryo, Mammalian / Q000276:immunology*; D006412:Hematopoietic Stem Cells / Q000276:immunology*; D007128:Immunoglobulin Fragments; D007143:Immunoglobulin Heavy Chains; D007075:Immunoglobulin M / Q000096:biosynthesis; D015851:Interleukin-7 / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D016513:Mice, SCID
D007128:Immunoglobulin Fragments; D007143:Immunoglobulin Heavy Chains; D007075:Immunoglobulin M; D015851:Interleukin-7; D019297:2,4-Dinitrophenol