title: Endomorphins 1 and 2 modulate chemotaxis, phagocytosis and superoxide anion production by microglia.
abstract: We evaluate the role of endomorphins 1 and 2 on microglial functions. Endomorphins 1 and 2 blocked phagocytosis of Escherichia coli. In addition, both markedly inhibited chemotaxis toward zymosan-activated serum. In contrast, when microglia was preincubated with these endomorphins, followed by incubation with LPS before stimulation with phorbol 12-myristate 13-acetate (PMA) at 200 nM, they potentiated superoxide anion production. Furthermore, when microglia was preincubated with these endomorphins together with PMA at 20 nM, followed by stimulation with PMA at 200 nM, superoxide anion production was potentiated. These results suggest that endomorphins 1 and 2 modulate phagocytosis, chemotaxis and superoxide anion production by microglia.
authors.affiliation: Department of Pharmacology, Osaka Dental University, 8-1 Kuzuhahanazono-cho, Osaka 573-1121, Hirakata, Japan. aazuma@cc.osaka-dent.ac.jp
authors.forename: Y
authors.initials: Y
authors.lastname: Azuma
authors.forename: K
authors.initials: K
authors.lastname: Ohura
authors.forename: P L
authors.initials: PL
authors.lastname: Wang
authors.forename: M
authors.initials: M
authors.lastname: Shinohara
mesh_terms: D000818:Animals; D001769:Blood / Q000187:drug effects; D001790:Blood Physiological Phenomena; D002633:Chemotaxis / Q000187:drug effects*; D004622:Embryo, Mammalian; D004926:Escherichia coli / Q000502:physiology; D017628:Microglia / Q000187:drug effects* / Q000502:physiology*; D009842:Oligopeptides / Q000494:pharmacology*; D010587:Phagocytosis / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D013481:Superoxides / Q000378:metabolism*; D015054:Zymosan / Q000494:pharmacology
chemical_list: D009842:Oligopeptides; C105293:endomorphin 1; D013481:Superoxides; C105294:endomorphin 2; D015054:Zymosan