Short-contact topical tretinoin therapy to stimulate granulation tissue in chronic wounds.
BACKGROUND
The use of retinoids in wound healing is increasing. It has been shown that retinoic acid reverses the inhibitory effects of glucocorticoids on wound healing and accelerates the formation of healthy granulation tissue. Pretreatment with tretinoin before epidermal injury such as chemical peeling and dermabrasion has shown accelerated wound healing. Enhanced healing of full-thickness skin wounds has also been demonstrated in early wound healing studies. However, tretinoin therapy can be quite irritating.


OBJECTIVE
Our purpose was to observe the clinical and histologic effects of topical tretinoin solution 0.05% applied directly to the wound beds of chronic leg ulcerations.


METHODS
We report on the cases of 5 patients with long-standing leg ulcerations. All were treated with topical tretinoin solution 0.05% applied directly to the wound bed. The tretinoin solution was left in contact with the ulcer bed for a maximum of 10 minutes daily and then rinsed with normal saline. Punch biopsy specimens were obtained from the wound beds at baseline and mid therapy. Standard wound care was continued throughout the study.


RESULTS
In this study we found that as early as 1 week after treatment with topical tretinoin solution 0.05%, there was increased granulation tissue first noted at the wound's edge. After 4 weeks of therapy with tretinoin, there was further stimulation of granulation tissue, new vascular tissue, and new collagen formation.


CONCLUSIONS
Short-contact tretinoin therapy is a novel modality in which to treat chronic ulcers and stimulate the formation of granulation tissue.
Paquette
D
D
Department of Dermatology & Skin Surgery, Roger Williams Medical Center, 50 Maude St., Providence, RI 02908, USA.
Badiavas
E
E
Falanga
V
V
D000279:Administration, Cutaneous; D000368:Aged; D002908:Chronic Disease; D005260:Female; D006097:Granulation Tissue / Q000378:metabolism*; D006801:Humans; D007641:Keratolytic Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D007871:Leg Ulcer / Q000188:drug therapy* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D014212:Tretinoin / Q000008:administration & dosage / Q000627:therapeutic use*; D014945:Wound Healing
D007641:Keratolytic Agents; D014212:Tretinoin