title: A moving DNA replication factory in Caulobacter crescentus.
abstract: The in vivo intracellular location of components of the Caulobacter replication apparatus was visualized during the cell cycle. Replisome assembly occurs at the chromosomal origin located at the stalked cell pole, coincident with the initiation of DNA replication. The replisome gradually moves to midcell as DNA replication proceeds and disassembles upon completion of DNA replication. Although the newly replicated origin regions of the chromosome are rapidly moved to opposite cell poles by an active process, the replisome appears to be an untethered replication factory that is passively displaced towards the center of the cell by the newly replicated DNA. These results are consistent with a model in which unreplicated DNA is pulled into the replication factory and newly replicated DNA is bidirectionally extruded from the complex, perhaps contributing to chromosome segregation.
authors.affiliation: Department of Developmental Biology, Beckman Center, Stanford University School of Medicine, Stanford, CA 94305-5329, USA.
authors.forename: R B
authors.initials: RB
authors.lastname: Jensen
authors.forename: S C
authors.initials: SC
authors.lastname: Wang
authors.forename: L
authors.initials: L
authors.lastname: Shapiro
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D016935:Caulobacter crescentus / Q000166:cytology / Q000235:genetics* / Q000378:metabolism; D002453:Cell Cycle; D002455:Cell Division; D015825:Chromosomes, Fungal / Q000235:genetics; D004265:DNA Helicases / Q000378:metabolism; D004261:DNA Replication; D053488:DnaB Helicases; D049452:Green Fluorescent Proteins; D007700:Kinetics; D008164:Luminescent Proteins / Q000235:genetics; D008957:Models, Genetic; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D018741:Replication Origin
chemical_list: D001426:Bacterial Proteins; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; C054562:yellow fluorescent protein, Bacteria; D049452:Green Fluorescent Proteins; D004265:DNA Helicases; D053488:DnaB Helicases