title: Szygium aromaticum (L.) Merr. Et Perry (Myrtaceae) flower bud induces apoptosis of p815 mastocytoma cell line.
abstract: This study was conducted to investigate SAFB-induced apoptosis of mast cells as it pertains to both its basic drug mechanism and the potential therapeutics of the pathologic conditions accompanying mast cell proliferation. SAFB induced many apoptotic manifestations as evidenced by changes in cell morphology, generation of DNA fragmentation, activation of caspase 3, and DNA hypoploidy. The reduction of mitochondrial membrane potential and the release of cytochrome c to cytosol were also demonstrated. However, reduction of mitochondrial membrane potential and cytochrome c release were not prevented by caspase inhibitor zVAD-fmk or PTP blockers such as bongkrekic acid and cyclosporin A. Expression levels of Bcl-2 and Fas remained unchanged following SAFB treatment. This results suggest that the clinical effect of SAFB may depend on the pharmacological mechanism regulating the demise of mast cells.
authors.affiliation: Department of Anatomy and Cell Biology, Dong-A University College of Medicine and Institute of Medical Science, Pusan, South Korea.
authors.forename: H I
authors.initials: HI
authors.lastname: Park
authors.forename: M H
authors.initials: MH
authors.lastname: Jeong
authors.forename: Y J
authors.initials: YJ
authors.lastname: Lim
authors.forename: B S
authors.initials: BS
authors.lastname: Park
authors.forename: G C
authors.initials: GC
authors.lastname: Kim
authors.forename: Y M
authors.initials: YM
authors.lastname: Lee
authors.forename: H M
authors.initials: HM
authors.lastname: Kim
authors.forename: K S
authors.initials: KS
authors.lastname: Yoo
authors.forename: Y H
authors.initials: YH
authors.lastname: Yoo
mesh_terms: D000818:Animals; D017209:Apoptosis; D004273:DNA, Neoplasm / Q000187:drug effects; D012515:Mast-Cell Sarcoma; D051379:Mice; D008928:Mitochondria / Q000187:drug effects / Q000502:physiology; D010936:Plant Extracts / Q000494:pharmacology; D019685:Rosales / Q000737:chemistry*; D014407:Tumor Cells, Cultured
chemical_list: D004273:DNA, Neoplasm; D010936:Plant Extracts