Cloning of parsley flavone synthase I.
A cDNA encoding flavone synthase I was amplified by RT-PCR from leaflets of Petroselinum crispum cv. Italian Giant seedlings and functionally expressed in yeast cells. The identity of the recombinant, 2-oxoglutarate-dependent enzyme was verified in assays converting (2S)-naringenin to apigenin.
Martens
S
S
Technische Universität München, Wissenschaftszentrum für Ernährung, Landnutzung und Umwelt, Department für Pflanzenwissenschaften, Lehrstuhl für Zierpflanzenbau, Am Hochanger 4, D-85350 Freising, Germany.
Forkmann
G
G
Matern
U
U
Lukacin
R
R
D047310:Apigenin; D003001:Cloning, Molecular; D018076:DNA, Complementary; D044950:Flavanones; D005419:Flavonoids / Q000378:metabolism; D006899:Mixed Function Oxygenases / Q000235:genetics* / Q000378:metabolism; D028528:Petroselinum / Q000201:enzymology*; D018515:Plant Leaves / Q000201:enzymology; D011994:Recombinant Proteins / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000201:enzymology
D018076:DNA, Complementary; D044950:Flavanones; D005419:Flavonoids; D011994:Recombinant Proteins; D047310:Apigenin; D006899:Mixed Function Oxygenases; C065998:flavone synthase I; C005273:naringenin