title: A Drosophila PIAS homologue negatively regulates stat92E.
abstract: Transcriptional activation by, and therefore the physiologic impact of, activated tyrosine-phosphorylated STATs (signal transducers and activators of transcription) may be negatively regulated by proteins termed PIAS (protein inhibitors of activated stats), as shown by previous experiments with mammalian cells in culture. Here, by using the genetic modifications in Drosophila, we demonstrate the in vivo functional interaction of the Drosophila homologues stat92E and a Drosophila PIAS gene (dpias). To this end we use a LOF allele and conditionally overexpressed dpias in JAK-STAT pathway mutant backgrounds. We conclude that the correct dpias/stat92E ratio is crucial for blood cell and eye development.
authors.affiliation: Laboratories of Molecular Cell Biology and Genetics, The Rockefeller University, New York, NY 10021, USA.
authors.forename: A
authors.initials: A
authors.lastname: Betz
authors.forename: N
authors.initials: N
authors.lastname: Lampen
authors.forename: S
authors.initials: S
authors.lastname: Martinek
authors.forename: M W
authors.initials: MW
authors.lastname: Young
authors.forename: J E
authors.initials: JE
authors.lastname: Darnell
mesh_terms: D000483:Alleles; D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000502:physiology*; D004268:DNA-Binding Proteins / Q000037:antagonists & inhibitors*; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000196:embryology / Q000235:genetics / Q000378:metabolism*; D005123:Eye / Q000196:embryology / Q000648:ultrastructure; D005124:Eye Abnormalities / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D019476:Insect Proteins / Q000235:genetics / Q000502:physiology*; D047908:Intracellular Signaling Peptides and Proteins; D053612:Janus Kinases; D009024:Morphogenesis; D016254:Mutagenesis, Insertional; D009374:Neoplasms, Experimental / Q000235:genetics; D010766:Phosphorylation; D050828:Protein Inhibitors of Activated STAT; D011499:Protein Processing, Post-Translational; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D011506:Proteins / Q000235:genetics / Q000502:physiology*; D011995:Recombination, Genetic; D050791:STAT Transcription Factors; D015398:Signal Transduction; D015534:Trans-Activators / Q000037:antagonists & inhibitors*; D014157:Transcription Factors
chemical_list: D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D019476:Insect Proteins; D047908:Intracellular Signaling Peptides and Proteins; D050828:Protein Inhibitors of Activated STAT; D011506:Proteins; D050791:STAT Transcription Factors; C105675:Stat92E protein, Drosophila; C118811:Su(var)2-10 protein, Drosophila; D015534:Trans-Activators; D014157:Transcription Factors; D011505:Protein-Tyrosine Kinases; D053612:Janus Kinases; C085845:hop protein, Drosophila