P8 expression is induced in acinar cells during chronic pancreatitis.
The p8 gene is barely expressed in the normal pancreas, but is overexpressed in acute pancreatitis. To elucidate the dynamic expression of p8 mRNA and its significance in the course of chronic pancreatitis, we investigated the p8 expression in spontaneous chronic pancreatitis in the WBN/Kob rat as well as in humans and arginine-treated rat pancreatic acinar AR4-2J cells. p8 mRNA was significantly increased at 12 weeks when chronic pancreatitis first appeared in the WBN/Kob rats. p8 was immunolocalized in the acinar cell nuclei. Acinar cell apoptosis was significantly increased at 12 and 20 weeks in the WBN/Kob rats. In AR4-2J cells, p8 mRNA was significantly induced at 4 hr after arginine addition. Apoptosis of AR4-2J cells was not increased during the strong expression of p8 mRNA. These results suggest that p8 is induced in the acinar cells during chronic pancreatitis as the self-defence mechanism against proapoptotic insults.
Motoo
Y
Y
Department of Internal Medicine and Medical Oncology, Cancer Research Institute, Kanazawa University, Japan.
Iovanna
J L
JL
Mallo
G V
GV
Su
S B
SB
Xie
M J
MJ
Sawabu
N
N
D000209:Acute-Phase Proteins / Q000378:metabolism; D000818:Animals; D000951:Antigens, Neoplasm; D017209:Apoptosis; D051792:Basic Helix-Loop-Helix Proteins; D014408:Biomarkers, Tumor; D002460:Cell Line; D002908:Chronic Disease; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D006133:Growth Substances / Q000378:metabolism*; D006801:Humans; D020287:In Situ Nick-End Labeling; D037181:Lectins, C-Type; D008297:Male; D009363:Neoplasm Proteins; D010179:Pancreas / Q000166:cytology / Q000378:metabolism* / Q000473:pathology; D010195:Pancreatitis / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D000073718:Pancreatitis-Associated Proteins; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction
D000209:Acute-Phase Proteins; D000951:Antigens, Neoplasm; D051792:Basic Helix-Loop-Helix Proteins; D014408:Biomarkers, Tumor; D004268:DNA-Binding Proteins; D006133:Growth Substances; D037181:Lectins, C-Type; D009363:Neoplasm Proteins; D000073718:Pancreatitis-Associated Proteins; D012333:RNA, Messenger; C491643:NUPR1 protein, human; C109741:Nupr1 protein, rat; C000612141:REG3A protein, human