Monitoring of biological responses of tumor cells after irradiation with 99mTc-MIBI--an in vitro study.
To explore the possibility to employ 99mTc-MIBI to monitor biological response of tumor cells after irradiation and to observe the relation between the radiation doses and the uptake levels of 99mTc-MIBI in tumor cells, the cells were irradiated with a single dose of 2 Gy, 10 Gy and 20 Gy respectively. The uptake of 99mTc-MIBI in each dosage group was determined before and 24, 48, 72 h after irradiation respectively. Apoptosis index (AI), plating efficiency (PE) of tumor cells was simultaneously determined. There was a positive correlation between uptake levels of 99mTc-MIBI and AI(r = -0.91, P < 0.05). A negative correlation was noted between the uptake levels and PE (r = -0.86, P < 0.05). It is suggested that 99mTc-MIBI may be used as a tracer to monitor the change of viability state of tumor cells after being irradiated with different doses.
Xia
J
J
Department of Nuclear Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030.
Wu
H
H
Zhao
M
M
Xianyu
Z
Z
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000473:pathology* / Q000532:radiotherapy; D002455:Cell Division / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D051379:Mice; D011834:Radiation Monitoring / Q000379:methods*; D017256:Technetium Tc 99m Sestamibi / Q000008:administration & dosage*; D014407:Tumor Cells, Cultured / Q000528:radiation effects
D017256:Technetium Tc 99m Sestamibi