[The new experimental ulcerative colitis model in rats induced by subserosal injection of acetic acid].
We have developed a new experimental ulcerative colitis model in rats. Topical pathological change of a round or a ellips shape was induced by subserosal injection of acetic acid (20%, 0.02 ml) into the middle colon of rats. The size of the induced ulcer could directly be measured using a caliper gauge, and the result was expressed as the ulcer area (mm2). We determined the concentration of leukotriene B4 (LTB4), which is one of important clinical factors, in the ulcer region and found that the quantity of LTB4 was well correlated with the size of the ulcer area. Histopathological studies of the ulcer region demonstrated that there were some morphological similarities to the human form of ulcerative colitis, characterized by edema, necrosis, inflammatory cell infiltration, crypt abscess and granulation tissue formation. Effects of 5-aminosalicylic acid and sodium prednisolone phosphate were investigated by intrarectal administration in this colitis model. The predominant improvement of colitis was obtained from both treatments in the ranges of the clinical doses of each drug. In conclusion, we suggest that this colitis model provides a new way for quantitative evaluation of the efficacy of new therapeutic agents for ulcerative colitis.
Kojima
R
R
Research Laboratories, Nisshin Kyorin Pharmaceutical Co., Ltd., 5-3-1, Tsurugaoka, Oi-machi, Iruma-gun, Saitama 356-8511, Japan.
Hamamoto
S
S
Moriwaki
M
M
Iwadate
K
K
Ohwaki
T
T
D019342:Acetic Acid / Q000008:administration & dosage; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use; D003093:Colitis, Ulcerative / Q000139:chemically induced* / Q000188:drug therapy / Q000473:pathology; D004195:Disease Models, Animal; D018836:Inflammation Mediators / Q000378:metabolism; D007975:Leukotriene B4 / Q000378:metabolism; D008297:Male; D019804:Mesalamine / Q000627:therapeutic use; D011239:Prednisolone / Q000031:analogs & derivatives* / Q000627:therapeutic use; D051381:Rats; D017208:Rats, Wistar
D000893:Anti-Inflammatory Agents; D018836:Inflammation Mediators; D007975:Leukotriene B4; D019804:Mesalamine; C009022:prednisolone phosphate; D011239:Prednisolone; D019342:Acetic Acid