title: Formaldehyde production by Tris buffer in peptide formulations at elevated temperature.
abstract: This technical note provides evidence for the degradation of Tris buffer in a peptide formulation stored at elevated temperature (70 degrees C). The buffer degrades to liberate formaldehyde, which is shown to react with the peptide tyrosine residue. Those involved in peptide/protein formulation should be aware of the possible instability in this common biological buffer.
authors.affiliation: Department of Pharmaceutical Chemistry, The University of Kansas, Lawrence, KS 66047, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Song
authors.forename: R L
authors.initials: RL
authors.lastname: Schowen
authors.forename: R T
authors.initials: RT
authors.lastname: Borchardt
authors.forename: E M
authors.initials: EM
authors.lastname: Topp
mesh_terms: D005557:Formaldehyde / Q000138:chemical synthesis*; D006358:Hot Temperature; D010455:Peptides / Q000737:chemistry*; D014325:Tromethamine / Q000737:chemistry*
chemical_list: D010455:Peptides; D014325:Tromethamine; D005557:Formaldehyde