title: Distribution of rotavirus-specific memory B cells in gut-associated lymphoid tissue after primary immunization.
abstract: We found previously that mice inoculated orally with simian rotavirus strain RRV developed virus-specific memory B cell responses 16 weeks after immunization that were greater than those found 6 weeks after immunization. Memory B cell responses were defined as the quantity of virus-specific IgA detected in small intestinal lamina propria (LP) fragment cultures of immunized mice at various intervals after challenge. Enhanced memory B cell responses correlated with enhanced protection against shedding. In order to understand better the delayed onset of rotavirus-specific memory B cell responses, a method was developed to determine the frequencies of rotavirus-specific memory B cells in gut-associated lymphoid tissues (GALT). We found that protection against rotavirus challenge was determined by the frequency of rotavirus-specific memory B cells in GALT LP.
authors.affiliation: Section of Infectious Diseases, The Children's Hospital of Philadelphia, Abramson Research Building, Room 1205A, 3516 Civic Center Blvd, Philadelphia, PA 19104, USA1.
authors.forename: Charlotte A
authors.initials: CA
authors.lastname: Moser
authors.affiliation: The University of Pennsylvania School of Medicine2 and The Wistar Institute of Anatomy and Biology3, Philadelphia, PA 19104, USA.|Section of Infectious Diseases, The Children's Hospital of Philadelphia, Abramson Research Building, Room 1205A, 3516 Civic Center Blvd, Philadelphia, PA 19104, USA1.
authors.forename: Paul A
authors.initials: PA
authors.lastname: Offit
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D001402:B-Lymphocytes / Q000276:immunology*; D005260:Female; D007114:Immunization; D007156:Immunologic Memory; D007413:Intestinal Mucosa / Q000276:immunology*; D008221:Lymphoid Tissue / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D012401:Rotavirus / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral