Analysis of p73 gene in meningiomas with deletion at 1p.
The p73 gene has been mapped to 1p36.33, a chromosome region that is frequently deleted in a wide variety of neoplasms including meningiomas. The protein encoded by p73 shows structural and functional similarities to p53 and may thus represent a candidate tumor suppressor gene. To determine whether p73 is involved in the development of meningiomas, we examined 30 meningioma samples with proven 1p deletion for mutations of p73. Sequence analysis of the entire coding region of the p73 gene revealed previously reported polymorphisms in eight cases. A tumor-specific missense mutation as a result of an A-to-G transition with an Asn204Ser change was found in one meningioma that nevertheless retained the normal allele. These results suggest that if p73 plays a role in meningioma carcinogenesis, it must be in a manner different from the Knudson two-hit model.
Lomas
J
J
Laboratorio de Oncogenética Molecular, Unidad de Investigación, Departamento de Cirugía Experimental, Hospital Universitario La Paz, Paseo Castellana 261, 28046 Madrid, Spain.
Bello
M J
MJ
Arjona
D
D
Gonzalez-Gomez
P
P
Alonso
M E
ME
de Campos
J M
JM
Vaquero
J
J
Ruiz-Barnes
P
P
Sarasa
J L
JL
Casartelli
C
C
Rey
J A
JA
D002872:Chromosome Deletion; D002878:Chromosomes, Human, Pair 1; D004268:DNA-Binding Proteins / Q000235:genetics*; D016147:Genes, Tumor Suppressor; D006801:Humans; D008577:Meningeal Neoplasms / Q000235:genetics*; D008579:Meningioma / Q000235:genetics*; D009687:Nuclear Proteins / Q000235:genetics*; D000072160:Tumor Protein p73; D025521:Tumor Suppressor Proteins
D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; C000606460:TP73 protein, human; D000072160:Tumor Protein p73; D025521:Tumor Suppressor Proteins