Juvenile hormone binding protein and transferrin from Galleria mellonella share a similar structural motif.
It has been previously suggested that juvenile hormone binding protein(s) (JHBP) belongs to a new class of proteins. In the search for other protein(s) that may contain structural motifs similar to those found in JHBP, hemolymph from Galleria mellonella (Lepidoptera) was chromatographed over a Sephadex G-200 column and resulting fractions were subjected to SDS-PAGE, transferred onto nitrocellulose membrane and scanned with a monoclonal antibody, mAb 104, against hemolymph JHBP. Two proteins yielded a positive reaction with mAb 104, one corresponding to JHBP and the second corresponding to a transferrin, as judged from N-terminal amino acid sequencing staining. Transferrin was purified to about 80% homogeneity using a two-step procedure including Sephadex G-200 gel filtration and HPLC MonoQ column chromatography. Panning of a random peptide display library and analysis with immobilized synthetic peptides were applied for finding a common epitope present in JHBP and the transferrin molecule. The postulated epitope motif recognized by mAb 104 in the JHBP sequence is RDTKAVN, and is localized at position 82-88.
Krzyzanowska
D
D
Division of Biochemistry, Institute of Organic Chemistry, Biochemistry and Biotechnology, Wrocław University of Technology, Poland.
Ozyhar
A
A
Lalik
A
A
Parkitna
J M
JM
Szkudlarek
J
J
Waśniowska
K
K
Lisowska
E
E
Kochman
M
M
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000941:Antigens / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D002352:Carrier Proteins / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002863:Chromogenic Compounds / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D018604:Epitope Mapping; D000939:Epitopes / Q000032:analysis; D006458:Hemolymph / Q000737:chemistry; D015151:Immunoblotting; D019476:Insect Proteins; D007915:Lepidoptera / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D008969:Molecular Sequence Data; D010802:Phylogeny; D020539:Sequence Analysis, Protein; D014168:Transferrin / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D014227:Triazines / Q000737:chemistry
D000911:Antibodies, Monoclonal; D000941:Antigens; D002352:Carrier Proteins; D002863:Chromogenic Compounds; D000939:Epitopes; D019476:Insect Proteins; D014168:Transferrin; D014227:Triazines; C035606:juvenile hormone-binding protein, insect; C031748:Ferene-S