title: Sertoli-stromal cell tumor of the ovary: immunohistochemical, ultrastructural, and genetic studies.
abstract: The Sertoli-stromal cell tumor (SSCT) of the ovary shows a histologic resemblance to developing or adult testes and is often associated with virilization caused by tumor-produced androgenic hormone. In spite of the unique manifestation of SSCT, detailed characteristics of this tumor are still obscure. The mechanism by which SSCT occurs has not yet been determined. Six SSCTs were studied immunohistochemically, ultrastructurally, and by polymerase chain reaction (PCR) for the presence of sex-determining region Y (SRY) gene and the X chromosome activation state. Immunohistochemically, Sertoli-like cells of SSCT were positive not only for alpha-inhibin but also low-molecular-weight cytokeratin. In control testes, the expression of alpha-inhibin and cytokeratin was limited to a Sertoli cell component and rete testis, respectively. Ultrastructurally, tumor cells composing hollow tubules had an elongated nucleus with deep indentation and annulate lamellae, which are characteristic structures of mature Sertoli cells. In addition, they had studded microvilli on the apical surface and frequent desmosomes, which are structures noted in the cells of rete testis. Histologically, tumor cells of hollow tubules sometimes pouted into the lumen, as did the cells of tubulae rete, entrance into rete testis from seminiferous tubules. All of these findings indicate that some tumor cells of a SSCT show simultaneous differentiation into both Sertoli cells and cells of rete testis. SRY gene was not detected in any cases, and the X chromosome activation pattern was the same as that of the female control.
authors.affiliation: Department of Pathology, Yamagata University School of Medicine, Yamagata, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Kato
authors.forename: M
authors.initials: M
authors.lastname: Fukase
authors.forename: I
authors.initials: I
authors.lastname: Ono
authors.forename: K
authors.initials: K
authors.lastname: Matsumoto
authors.forename: E
authors.initials: E
authors.lastname: Okazaki
authors.forename: T
authors.initials: T
authors.lastname: Motoyama
mesh_terms: D000951:Antigens, Neoplasm / Q000032:analysis; D014408:Biomarkers, Tumor; D017931:DNA Primers / Q000737:chemistry; D004273:DNA, Neoplasm / Q000032:analysis; D004268:DNA-Binding Proteins / Q000235:genetics; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D007265:Inhibins / Q000032:analysis; D007633:Keratins / Q000032:analysis; D008297:Male; D018895:Microsatellite Repeats; D009687:Nuclear Proteins; D010051:Ovarian Neoplasms / Q000737:chemistry / Q000235:genetics / Q000473:pathology* / Q000601:surgery; D016133:Polymerase Chain Reaction; D018312:Sex Cord-Gonadal Stromal Tumors / Q000737:chemistry / Q000235:genetics / Q000473:pathology* / Q000601:surgery; D012732:Sex Determination Analysis / Q000379:methods; D051876:Sex-Determining Region Y Protein; D013736:Testicular Neoplasms / Q000737:chemistry / Q000235:genetics / Q000473:pathology / Q000601:surgery; D014157:Transcription Factors; D014960:X Chromosome
chemical_list: D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D017931:DNA Primers; D004273:DNA, Neoplasm; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; C497745:SRY protein, human; D051876:Sex-Determining Region Y Protein; D014157:Transcription Factors; C094377:inhibin-alpha subunit; D007265:Inhibins; D007633:Keratins