Sensitive chemiluminescence immunoassay for the determination of rat serum alpha1-acid glycoprotein.
We present the establishment of a sensitive immunoassay for the determination of alpha1-acid glycoprotein (orosomucoid, AGP) in rat serum. The assay is based upon antigen capture by surface-immobilized specific polyclonal rabbit anti-AGP antibodies with biotinylated rat AGP (rAGP) as the tracer, and formatted as competitive enzyme immunoassay. Signaling is performed by streptavidin-conjugated horseradish peroxidase. Enzyme activity is quantified by an enhanced chemiluminometric method, allowing the sensitive detection of rAGP serum levels in small sample volumes.
Metzger
J
J
Institut für Klinische Chemie und Pathobiochemie, Klinikum rechts der Isar, Technische Universität München, Germany.
Blobner
M
M
Luppa
P B
PB
D000818:Animals; D000906:Antibodies; D007124:Immunoenzyme Techniques / Q000379:methods* / Q000706:statistics & numerical data; D008163:Luminescent Measurements; D009961:Orosomucoid / Q000032:analysis* / Q000276:immunology; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley; D012680:Sensitivity and Specificity; D018805:Sepsis / Q000097:blood
D000906:Antibodies; D009961:Orosomucoid