title: Effects of light and darkness on cell deaths in damaged retinal ganglion cells of the carp retina.
abstract: Effects of light and darkness on the apoptosis of retinal ganglion cells (RGCs) in young carp were measured by TUNEL method after transection of the optic nerve. Following the operation, the fish were kept under one of four regimens; constant darkness (DD), constant light (LL), 12 hr light and 12 hr dark (LD) and 3 hr of flickering light followed by 21 hr in the dark (FL). On day 3, the highest ratio of apoptotic RGCs was seen under conditions of DD, followed by LL, LD, and FL. On day 6, the percentages of apoptotic RGCs were lower under every experimental condition than what they had been earlier on day 3, but the same ranking order was maintained. Immunohistochemically it could be shown that phosphorylated ERKs were more intensively localized in FL rather than DD retinas. The results suggest that illumination regimens, and in particular cyclic diurnal light/dark changes, have an influence on the degree of apoptosis of damaged RGCs, and that inhibition of apoptosis is correlated with the higher expression of phosphorylated ERKs.
authors.affiliation: Department of Anatomy, Yokohama City University School of Medicine, 3-9 Fukuura, Kanazawa-ku, Yokohama 236-0004, Japan. dezawa@med.yokohama-cu.ac.jp.
authors.forename: M
authors.initials: M
authors.lastname: Dezawa
authors.forename: X
authors.initials: X
authors.lastname: Mo
authors.forename: T
authors.initials: T
authors.lastname: Oshitari
authors.forename: M
authors.initials: M
authors.lastname: Takano
authors.forename: V B
authors.initials: VB
authors.lastname: Meyer-Rochow
authors.forename: H
authors.initials: H
authors.lastname: Sawada
authors.forename: E
authors.initials: E
authors.lastname: Eguchi
mesh_terms: D000221:Adaptation, Ocular / Q000502:physiology*; D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D002347:Carps; D003623:Dark Adaptation / Q000502:physiology*; D003624:Darkness; D005455:Fluorescent Antibody Technique; D020287:In Situ Nick-End Labeling; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D020221:Optic Nerve Injuries / Q000473:pathology* / Q000503:physiopathology; D010775:Photic Stimulation; D012165:Retinal Ganglion Cells / Q000201:enzymology / Q000473:pathology*
chemical_list: D020928:Mitogen-Activated Protein Kinases