title: Localization of CYP86B1 in the outer envelope of chloroplasts.
abstract: CYP86B1 was cloned from a cDNA library and the protein expressed in E. coli. The protein gave the expected carbon monoxide difference spectrum. Using in vitro import assays with isolated pea chloroplasts, CYP86B1 was shown to be associated with the outer chloroplastic envelope membrane. This study provides the first direct evidence for a chloroplast-localized cytochrome P450-dependent monooxygenase.
authors.affiliation: Department of Botany and Plant Pathology, Purdue University, W. Lafayette, IN 47907, USA.
authors.forename: C J
authors.initials: CJ
authors.lastname: Watson
authors.forename: J E
authors.initials: JE
authors.lastname: Froehlich
authors.forename: C A
authors.initials: CA
authors.lastname: Josefsson
authors.forename: C
authors.initials: C
authors.lastname: Chapple
authors.forename: F
authors.initials: F
authors.lastname: Durst
authors.forename: I
authors.initials: I
authors.lastname: Benveniste
authors.forename: R C
authors.initials: RC
authors.lastname: Coolbaugh
mesh_terms: D000595:Amino Acid Sequence; D029681:Arabidopsis Proteins; D002736:Chloroplasts / Q000737:chemistry* / Q000235:genetics; D003001:Cloning, Molecular; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis* / Q000235:genetics; D018076:DNA, Complementary; D018744:DNA, Plant; D004926:Escherichia coli; D007425:Intracellular Membranes / Q000737:chemistry; D008969:Molecular Sequence Data; D018532:Pisum sativum / Q000737:chemistry* / Q000235:genetics; D010940:Plant Proteins / Q000032:analysis / Q000235:genetics
chemical_list: D029681:Arabidopsis Proteins; D018076:DNA, Complementary; D018744:DNA, Plant; D010940:Plant Proteins; D003577:Cytochrome P-450 Enzyme System; C438008:CYP86B1 protein, Arabidopsis