[cGMP-independent effect of nitric oxide on contractility and intracellular calcium level of rat tail artery vascular smooth muscles].
The effects of nitric oxide (NO, 10(-6) M) on contractility and intracellular calcium level ([Ca2+]i) of rat tail artery smooth muscles in control and under inhibition of soluble guanylate cyclase (sGC) with 6-anilino-5,8-quinolinedione (LY83583, 10(-6) M) are investigated. NO-induced relaxations of vascular smooth muscles comprised 61.01 +/- 5.56% of maximum induced amplitude of K(+)-contracture, and decreases in [Ca2+]i comprised 66.35 +/- 11.33%. Under the inhibition of sGC with LY83583 NO-induced relaxations of vascular smooth muscles comprised 29.41 +/- 5.17% of maximum induced amplitude of K(+)-contracture, and decreases in [Ca2+]i comprised 53.68 +/- 16.93%. Thus, cGMP-independent relaxation of vascular smooth muscle and decrease in [Ca2+]i of rat tail artery is confirmed.
Solovĭov
A I
AI
Institute of Pharmacology and Toxicology, Academy of Medical Sciences of Ukraine, Kiev. Bogomoletz Institute of Physiology, National Academy of Sciences of Ukraine, Kiev.
Lehen'kyĭ
V V
VV
Zelens'kyĭ
S M
SM
Moĭbenko
O O
OO
Stefanov
O V
OV
D000634:Aminoquinolines / Q000494:pharmacology; D000818:Animals; D001158:Arteries / Q000187:drug effects / Q000502:physiology; D002118:Calcium / Q000378:metabolism*; D006152:Cyclic GMP / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006162:Guanylate Cyclase / Q000037:antagonists & inhibitors; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects* / Q000502:physiology; D009131:Muscle, Smooth, Vascular / Q000187:drug effects* / Q000502:physiology; D009569:Nitric Oxide / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D013623:Tail / Q000098:blood supply*
D000634:Aminoquinolines; D004791:Enzyme Inhibitors; D009569:Nitric Oxide; C041715:6-anilino-5,8-quinolinedione; D006162:Guanylate Cyclase; D006152:Cyclic GMP; D002118:Calcium
tsHMF-nezalezhnyĭ vplyv oksydu azotu na skorotlyvu aktyvnist' i vmist vnutrishn'oklitynnoho Ca(2+) hladen'kykh m'iaziv khvostovoï arteriï shchura.