Short-term effects of thyroid hormones on lipogenic enzymes and 14C-acetate incorporation into various lipid classes: in vivo and in vitro studies.
Short-term effect of 3,5,3'-triiodothyronine (T3) and 3,5-diiodothyronine (T2) on lipid metabolism in the liver of Anabas testudineus was examined. In vivo injections of both T3 and T2 at a concentration of 10 ng/g body weight increased malic enzyme (ME), glucose-6-phosphate dehydrogenase (G6PDH) and 6-phosphogluconate dehydrogenase (6PGDH) activity compared to 6-propylthiouracil (6-PTU) treated group. Treatment of 6-PTU results in the accumulation 14C-acetate into fat and thyroid hormones' treatment reduce it. In vitro experiments show that malic enzyme activity is augmented only by high concentration of T3 (10(-7) M) where as all concentrations of T2 increase its activity. In vitro studies with T3 showed a biphasic effect on cholesterol content. Conversely T2 in vitro, reduced cholesterol content with all concentrations. From these results it can be concluded that both T3 and T2 have short-term effect on lipid metabolism in Anabas.
Varghese
S
S
Department of Zoology, University of Kerala, Kariavattom, Thiruvananthapuram-695 581, India.
Shameena
B
B
Sudhakaran
P R
PR
Oommen
O V
OV
D019342:Acetic Acid / Q000378:metabolism; D000818:Animals; D002784:Cholesterol / Q000378:metabolism; D004104:Diiodothyronines / Q000494:pharmacology; D005260:Female; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D066298:In Vitro Techniques; D007521:Isocitrate Dehydrogenase / Q000378:metabolism; D050356:Lipid Metabolism; D008291:Malate Dehydrogenase / Q000378:metabolism; D050538:Malate Dehydrogenase (NADP+); D010473:Perciformes / Q000378:metabolism; D010734:Phosphogluconate Dehydrogenase / Q000378:metabolism; D013963:Thyroid Hormones / Q000494:pharmacology*; D014284:Triiodothyronine / Q000494:pharmacology
D004104:Diiodothyronines; D013963:Thyroid Hormones; D014284:Triiodothyronine; C030103:3,5-diiodothyronine; D002784:Cholesterol; D008291:Malate Dehydrogenase; D007521:Isocitrate Dehydrogenase; C039355:isocitrate dehydrogenase (NADP+); D010734:Phosphogluconate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D050538:Malate Dehydrogenase (NADP+); D019342:Acetic Acid