Fast clean-up for polychlorinated dibenzo-p-dioxins, dibenzofurans and coplanar polychlorinated biphenyls analysis of high-fat-content biological samples.
A fast clean-up procedure for the low level analysis of polychlorinated dibenzo-p-dioxins. polychlorinated dibenzofurans and coplanar polychlorinated biphenyls in highly fatty biological matrices using high capacity disposable multi-layer silica columns is presented. Results were compared with gel permeation chromatography for removal of lipids. Analytical criteria such as recovery rates, repeatability, reproducibility and robustness are evaluated through a broad range of biological matrices and reference materials analysis. The final proposed procedure for the complete analysis, including pressurized liquid extraction, Power-Prep system clean-up and GC-high-resolution MS analysis requires only 48 h, and allows the simultaneous preparation of up to 10 samples.
Focant
J F
JF
Mass Spectrometry Laboratory, University of Liege, Belgium. jf.focant@ulg.ac.be
Eppe
G
G
Pirard
C
C
De Pauw
E
E
D001572:Benzofurans / Q000032:analysis*; D002850:Chromatography, Gel; D005223:Fats / Q000737:chemistry*; D011078:Polychlorinated Biphenyls / Q000032:analysis*; D000072317:Polychlorinated Dibenzodioxins / Q000031:analogs & derivatives* / Q000032:analysis*; D011786:Quality Control; D012015:Reference Standards; D015203:Reproducibility of Results
D001572:Benzofurans; D005223:Fats; D000072317:Polychlorinated Dibenzodioxins; C023614:dibenzofuran; D011078:Polychlorinated Biphenyls