title: Immunohistochemical profile of galectin-8 expression in benign and malignant tumors of epithelial, mesenchymatous and adipous origins, and of the nervous system.
abstract: This study aims to investigate whether the immunohistochemical expression of galectin-8 could be used as a diagnostic marker in tumor tissues of various histogenetic origins including specimens from epithelial (n=145), mesenchymatous (n=16), adipous (n=10) and central and peripheral nervous system (n=25) tissue, and 4 mesotheliomas. Immunohistochemical reactions were carried out with a polyclonal anti-galectin-8 antibody and histological slides from tissues derived from the files of the Laboratory of Anatomopathology of University Erasmus Hospital, Brussels. Formalin-fixed paraffin-embedded tissues of 45 normal cases as well as 41 benign and 114 malignant tumors were studied. Marked decreases in immunohistochemical galectin-8 expression were observed in colon (p=0.001), pancreas (p=0.007), liver (p=0.0008), skin (p=0.002) and larynx (p=0.02) tissue when comparing malignant tissue to normal tissue and/or benign tumors. The reverse relationship was observed for breast tissue (p=0.007). No statistically significant differences (p>0.05) were detected when comparing normal tissue and/or benign to malignant tumors in lung, bladder, kidney, prostate and stomach tissue. Significant galectin-8 expression was also measured in non-epithelial tissue including tumors of the central and peripheral nervous system as well as in skeletal muscle and mesotheliomas. Immunohistochemical monitoring of galectin-8 thus reveals an organ-type-dependent regulation of expression upon malignant transformation of various tissue types of epithelial origin. This observation will prompt further studies to delineate any relationship with prognosis.
authors.affiliation: Laboratory of Histopathology, Faculty of Medicine, Université Libre de Bruxelles, Belgium.
authors.forename: A
authors.initials: A
authors.lastname: Danguy
authors.forename: S
authors.initials: S
authors.lastname: Rorive
authors.forename: C
authors.initials: C
authors.lastname: Decaestecker
authors.forename: Y
authors.initials: Y
authors.lastname: Bronckart
authors.forename: H
authors.initials: H
authors.lastname: Kaltner
authors.forename: Y R
authors.initials: YR
authors.lastname: Hadari
authors.forename: R
authors.initials: R
authors.lastname: Goren
authors.forename: Y
authors.initials: Y
authors.lastname: Zich
authors.forename: M
authors.initials: M
authors.lastname: Petein
authors.forename: I
authors.initials: I
authors.lastname: Salmon
authors.forename: H J
authors.initials: HJ
authors.lastname: Gabius
authors.forename: R
authors.initials: R
authors.lastname: Kiss
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism; D001940:Breast / Q000378:metabolism; D001943:Breast Neoplasms / Q000378:metabolism; D016022:Case-Control Studies; D004848:Epithelium / Q000378:metabolism; D005260:Female; D037161:Galectins; D006801:Humans; D007150:Immunohistochemistry; D037102:Lectins / Q000378:metabolism*; D008297:Male; D008648:Mesoderm / Q000378:metabolism; D018205:Neoplasms, Adipose Tissue / Q000378:metabolism* / Q000473:pathology; D009375:Neoplasms, Glandular and Epithelial / Q000378:metabolism* / Q000473:pathology; D009420:Nervous System / Q000378:metabolism; D009423:Nervous System Neoplasms / Q000378:metabolism* / Q000473:pathology
chemical_list: D037161:Galectins; C091991:LGALS8 protein, human; D037102:Lectins