title: Cytochemical study of Streptococcus agalactiae and macrophage interaction.
abstract: Light and electron microscopy were used to analyse the process of interaction of Streptococcus agalactiae (serotypes Ia, III, and V) with resident and activated mouse peritoneal macrophages. Transmission electron microscopy showed that adherence of the S. agalactiae serotype Ia, but not III and V serotypes, to the surface of activated macrophages triggers the respiratory oxidative burst as revealed by the presence of reduced nicotinamide adenine dinucleotide (phosphate) [NAD(P)H]-oxidase in the phagocytic vacuoles. Fusion of macrophage lysosomes with bacteria-containing phagocytic vacuoles was observed in macrophages treated with Lucifer yellow as well as by localization of acid phosphatase for all serotypes.
authors.affiliation: Departamento de Biologia Celular e Genética, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, Brasil.
authors.forename: C F
authors.initials: CF
authors.lastname: Teixeira
authors.forename: N L
authors.initials: NL
authors.lastname: Azevedo
authors.forename: T M
authors.initials: TM
authors.lastname: Carvalho
authors.forename: J
authors.initials: J
authors.lastname: Fuentes
authors.forename: P E
authors.initials: PE
authors.lastname: Nagao
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D006651:Histocytochemistry; D007546:Isoquinolines; D008247:Lysosomes / Q000382:microbiology / Q000502:physiology / Q000648:ultrastructure; D008262:Macrophage Activation; D017737:Macrophages, Peritoneal / Q000166:cytology* / Q000382:microbiology* / Q000648:ultrastructure; D051379:Mice; D018613:Microscopy, Confocal; D008854:Microscopy, Electron; D019255:NADPH Oxidases / Q000378:metabolism; D010588:Phagosomes / Q000382:microbiology / Q000502:physiology / Q000648:ultrastructure; D016897:Respiratory Burst; D013292:Streptococcus agalactiae / Q000145:classification / Q000166:cytology* / Q000502:physiology* / Q000648:ultrastructure
chemical_list: D007546:Isoquinolines; C017475:lucifer yellow; D019255:NADPH Oxidases; D000135:Acid Phosphatase