Transcriptome of channel catfish (Ictalurus punctatus): initial analysis of genes and expression profiles of the head kidney.
Analysis of expressed sequence tags (ESTs) is an efficient approach for gene discovery, expression profiling, and development of resources useful for functional genomics studies. As part of the transcriptome analysis in channel catfish (Ictalurus punctatus), we have conducted EST analysis using a cDNA library made from the head kidney. We analysed 2228 EST clones. Orthologues were established for 1495 (67.1%) clones representing 748 genes, of which 545 (36.5%) clones were singletons. The remaining 733 (32.9%) clones represent unknown gene clones, for which the number of genes has not yet been determined.
Cao
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The Fish Molecular Genetics and Biotechnology Laboratory, Department of Fisheries and Allied Aquacultures, Auburn University, Auburn, AL 36849, USA.
Kocabas
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Ju
Z
Z
Karsi
A
A
Li
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P
Patterson
A
A
Liu
Z
Z
D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary / Q000737:chemistry; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D007059:Ictaluridae / Q000235:genetics* / Q000378:metabolism; D007668:Kidney / Q000378:metabolism / Q000502:physiology*; D008969:Molecular Sequence Data; D012313:RNA / Q000737:chemistry / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
D018076:DNA, Complementary; D012313:RNA