Increased elastin expression in astrocytes of the lamina cribrosa in response to elevated intraocular pressure.
OBJECTIVE
To determine whether abnormal elastin synthesis in the glaucomatous optic nerve head and lamina cribrosa is due to elevated intraocular pressure (IOP) or secondary to axonal injury, monkeys with elevated IOP and with optic nerve transection were compared.


METHODS
Unilateral, chronic elevated IOP was induced in 11 rhesus monkeys by laser scarification of the trabecular meshwork. IOP was monitored weekly and maintained within 25 to 45 mm Hg for 7 to 36 weeks. In 6 monkeys, unilateral, optic nerve transection was performed, and monkeys were killed after 4 weeks. Optic nerve damage was assessed by stereoscopic slit-lamp biomicroscopy and fundus photography and by confocal scanning laser ophthalmoscopy. The eyes were enucleated and processed for immunohistochemistry and in situ hybridization and for electron microscopic immunogold detection of elastin. Axonal loss was evaluated in cross sections of the optic nerve stained with phenylenediamine.


RESULTS
Compared with normal contralateral controls, the lamina cribrosa of eyes with elevated IOP exhibited markedly increased elastin and the presence of elastotic aggregates in the extracellular matrix and upregulation of elastin mRNA in the astrocytes. In transected eyes, elastin appeared as fine fibers in the lamina cribrosa, without elastotic aggregates, and without new synthesis or abnormal deposition of elastin. At the transected site, new synthesis of elastin was present in the pia mater but not in astrocytes in the glial scar.


CONCLUSIONS
This study demonstrates that abnormal elastin synthesis in experimental glaucomatous optic neuropathy in the monkey is specific to elevated IOP and not secondary to axonal loss. The mechanisms by which elevated IOP induces enhanced elastin synthesis in laminar astrocytes are unknown but differ from those involved in acute axonal injury such as transection, where inflammation and breakdown of the blood-nerve barrier occur.
Pena
J D
JD
Department of Ophthalmology and Visual Sciences, Washington University School of Medicine, St. Louis, Missouri 63110, USA.
Agapova
O
O
Gabelt
B T
BT
Levin
L A
LA
Lucarelli
M J
MJ
Kaufman
P L
PL
Hernandez
M R
MR
D000818:Animals; D000911:Antibodies, Monoclonal; D001253:Astrocytes / Q000378:metabolism*; D004549:Elastin / Q000096:biosynthesis* / Q000235:genetics; D005109:Extracellular Matrix / Q000378:metabolism; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D005901:Glaucoma / Q000378:metabolism* / Q000473:pathology; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D017403:In Situ Hybridization; D007429:Intraocular Pressure; D008253:Macaca mulatta; D008297:Male; D009798:Ocular Hypertension / Q000378:metabolism / Q000473:pathology; D009898:Optic Disk / Q000378:metabolism*; D009900:Optic Nerve / Q000601:surgery; D020221:Optic Nerve Injuries / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D015854:Up-Regulation
D000911:Antibodies, Monoclonal; D005904:Glial Fibrillary Acidic Protein; D012333:RNA, Messenger; D004549:Elastin