[Adenine reutilization as a cause of increased ATP concentration in erythrocytes of patients with chronic renal failure].
Abnormalities of adenine nucleotide metabolism are observed in erythrocytes of patients with chronic renal failure (CRF) and the elevated ATP concentration is the most impressive one. In humans, adenine and/or adenosine reutilization is the only source of purine moiety used to erythrocyte adenine nucleotide synthesis. In the present study we have focused on the role of adenine as a substrate for the intraerythrocyte ATP production. 10 patients with CRF and 10 healthy volunteers (control group) were included into the study. Using HPLC, the measurements were performed in plasma and erythrocyte extracts. We observed a few fold higher adenine concentration in both plasma and erythrocytes of patients with CRF when compared with control group. There was also elevated an intraerythrocyte ATP concentration in the studied group of patients. Moreover, we have found a positive correlations between a) plasma creatinine concentration and plasma adenine concentration, b) plasma creatinine concentration and erythrocyte adenine concentration, c) plasma adenine concentration and intraerythrocyte ATP concentration. It appears that increased adenine reutilization could be a principle reason of the increased ATP synthesis in erythrocytes of patients with CRF.
Słomińska
E
E
Katedra i Zakład Biochemii AM w Gdańsku.
Szołkiewicz
M
M
Rutkowski
B
B
Swierczyński
J
J
D000225:Adenine / Q000378:metabolism* / Q000627:therapeutic use*; D000255:Adenosine Triphosphate / Q000096:biosynthesis / Q000097:blood*; D000328:Adult; D000368:Aged; D004912:Erythrocytes / Q000378:metabolism*; D005260:Female; D006801:Humans; D007676:Kidney Failure, Chronic / Q000188:drug therapy* / Q000378:metabolism*; D008297:Male; D008875:Middle Aged
D000255:Adenosine Triphosphate; D000225:Adenine
Reutylizacja adeniny jedna z przyczyn wzrostu stezenia ATP w erytrocytach pacjentów z przewlekła niewydolnościa nerek.