title: A rat model of acute lung injury induced by cardiopulmonary bypass.
abstract: Impaired lung function is still a major complication after cardiac surgery with cardiopulmonary bypass. The purpose of the present study was to develop an experimental model of acute pulmonary injury induced by cardiopulmonary bypass in Wistar rats. Cardiopulmonary bypass was performed for 60 min using a non-pulsatile roller pump and a membrane oxygenator (n = 8 for cardiopulmonary bypass group and n = 7 for control rats). We measured tracheal pressure, airflow, and lung volume changes and obtained pulmonary resistance and dynamic elastance. After the cardiopulmonary bypass, lungs were submitted to a quick-freezing protocol and morphometric analysis was performed. There was a time-dependent increase in dynamic elastance, but not pulmonary resistance, only in the rats submitted to cardiopulmonary bypass (P = 0.005). Lungs from animals submitted to cardiopulmonary bypass showed significantly more alveolar hemorrhage (P = 0.025) and edema (P = 0.021), as well as perivascular edema (P = 0.003) when compared to control rats. In our experimental model, rats submitted to cardiopulmonary bypass developed acute pulmonary changes similar to the early phase of acute pulmonary distress syndrome. Cardiopulmonary bypass resulted in an increase in pulmonary elastance without changes in resistance. This experimental model is suitable for studies concerning the mechanisms of acute lung injury induced by cardiopulmonary bypass.
authors.affiliation: Department of Medicine, School of Medicine, University of Sao Paulo, SP, Brazil.
authors.forename: D F
authors.initials: DF
authors.lastname: Senra
authors.forename: M
authors.initials: M
authors.lastname: Katz
authors.forename: G H
authors.initials: GH
authors.lastname: Passerotti
authors.forename: F M
authors.initials: FM
authors.lastname: Arantes-Costa
authors.forename: M L
authors.initials: ML
authors.lastname: Garcia
authors.forename: P H
authors.initials: PH
authors.lastname: Saldiva
authors.forename: M A
authors.initials: MA
authors.lastname: Martins
mesh_terms: D000818:Animals; D002315:Cardiopulmonary Bypass / Q000009:adverse effects*; D004195:Disease Models, Animal; D008168:Lung / Q000473:pathology* / Q000503:physiopathology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar