Anaerobic mineralization of stable-isotope-labeled 2-methylnaphthalene.
An active sulfate-reducing consortium that degrades 2-methylnaphthalene (2-MNAP) at rates of up to 25 microM x day(-1) was established. Degradation was inhibited in the presence of molybdate and ceased in the absence of sulfate. As much as 87% of 2-[14C]MNAP was mineralized to 14CO2. 2-Naphthoic acid (2-NA) was detected as a metabolite, and incubation with either deuterated 2-MNAP or [13C]bicarbonate indicates that 2-NA is the result of oxidation of the methyl group. Also detected were carboxylated 2-MNAPs, suggesting the presence of an alternative pathway for 2-MNAP degradation.
Sullivan
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Biotechnology Center for Agriculture and the Environment, Cook College, Rutgers, the State University of New Jersey, New Brunswick, New Jersey 08901, USA.
Zhang
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Phelps
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Young
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D000693:Anaerobiosis; D001421:Bacteria, Anaerobic / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002250:Carbon Radioisotopes / Q000378:metabolism; D005618:Fresh Water / Q000382:microbiology*; D009281:Naphthalenes / Q000378:metabolism*; D010084:Oxidation-Reduction; D013379:Substrate Specificity; D013455:Sulfur / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000378:metabolism*
D002250:Carbon Radioisotopes; D009281:Naphthalenes; D014874:Water Pollutants, Chemical; D013455:Sulfur; C027384:2-methylnaphthalene