Random amplification of polymorphic DNA and phenotypic typing of Zimbabwean isolates of Pasteurella multocida.
Eighty-one isolates presumptively identified as Pasteurella multocida from a variety of diseases in animals in Zimbabwe were subjected to biochemical characterization, capsular typing and RAPD analysis. The majority of isolates (over 80%) were assigned into named taxa and were predominantly P. multocida subsp. multocida and P. multocida subsp. septica, whilst the remainder were unassigned. Serogroup A was predominant among the three capsular types (A, B and D) of P. multocida detected. Three main RAPD clusters and three subclusters were observed among the majority of isolates (93.8%), whilst the remainder was found to be weakly related. Nine different groups of strains with similar RAPD profiles (100% similarity) were also observed. The reference strain of capsular serogroup F clustered with the reference strain of P. multocida subsp. septica, whilst all other serogroups clustered with reference strains of subsp. multocida and gallicida. Notably, serogroups A and D were observed to be closely related to the reference strain of subsp. multocida. The relationship between biotype, capsular type, host origin and disease manifestation was not clear-cut. However, most pig isolates of subsp. multocida clustered together as did most cattle isolates of subsp. multocida. RAPD tended to separate subsp. multocida from septica.
Dziva
F
F
Faculty of Veterinary Science, University of Zimbabwe, P.O. Box MP167, Mount Pleasant, Harare, Zimbabwe. fdziva@compcentre.uz.ac.zw
Christensen
H
H
Olsen
J E
JE
Mohan
K
K
D000818:Animals; D016667:Bacterial Capsules; D015373:Bacterial Typing Techniques; D002417:Cattle / Q000382:microbiology; D004269:DNA, Bacterial / Q000032:analysis*; D010326:Pasteurella Infections / Q000382:microbiology / Q000662:veterinary*; D016979:Pasteurella multocida / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D010641:Phenotype; D019105:Random Amplified Polymorphic DNA Technique; D012703:Serotyping; D013552:Swine / Q000382:microbiology
D004269:DNA, Bacterial