[Clone and expression of human soluble CD14 and study of its function].
Human soluble CD14(sCD14) cDNA fragment was amplified using total RNA extracted from U937 cells by RT-PCR of sCD14 gene, and the recombinant expression plasmid pEF1/HisC/sCD14 348aa was constructed. Then the expression in eukaryotic cell was carry out by liposome transfection method. It demonstrated that the expression level was relatively high by scanning map identification. The expressed product was purified by immunoaffinity chromatography and the purity was above 90%. The changes of CD14 brought by LPS stimulating U937 cell proved the product had the function of combine with LPS.
Bai
J
J
Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China. haku@263.net
Yin
J
J
Wang
Z D
ZD
Wang
W
W
Wang
Z Z
ZZ
Song
W
W
D003001:Cloning, Molecular; D006801:Humans; D018950:Lipopolysaccharide Receptors / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D008070:Lipopolysaccharides / Q000378:metabolism; D010957:Plasmids; D011994:Recombinant Proteins / Q000302:isolation & purification
D018950:Lipopolysaccharide Receptors; D008070:Lipopolysaccharides; D011994:Recombinant Proteins