title: Regulation of Wnt signaling and embryo patterning by an extracellular sulfatase.
abstract: The developmental signaling functions of cell surface heparan sulfate proteoglycans (HSPGs) are dependent on their sulfation states. Here, we report the identification of QSulf1, the avian ortholog of an evolutionarily conserved protein family related to heparan-specific N-acetyl glucosamine sulfatases. QSulf1 expression is induced by Sonic hedgehog in myogenic somite progenitors in quail embryos and is required for the activation of MyoD, a Wnt-induced regulator of muscle specification. QSulf1 is localized on the cell surface and regulates heparan-dependent Wnt signaling in C2C12 myogenic progenitor cells through a mechanism that requires its catalytic activity, providing evidence that QSulf1 regulates Wnt signaling through desulfation of cell surface HSPGs.
authors.affiliation: Department of Basic Veterinary Sciences, The Royal Veterinary College, University of London, Royal College Street, London NW1 OTU, UK.
authors.forename: G K
authors.initials: GK
authors.lastname: Dhoot
authors.forename: M K
authors.initials: MK
authors.lastname: Gustafsson
authors.forename: X
authors.initials: X
authors.lastname: Ai
authors.forename: W
authors.initials: W
authors.lastname: Sun
authors.forename: D M
authors.initials: DM
authors.lastname: Standiford
authors.forename: C P
authors.initials: CP
authors.lastname: Emerson
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D019521:Body Patterning; D016466:CHO Cells; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D003001:Cloning, Molecular; D018920:Coculture Techniques; D006224:Cricetinae; D004625:Embryo, Nonmammalian / Q000378:metabolism; D047108:Embryonic Development; D053823:Hedgehog Proteins; D019812:Heparan Sulfate Proteoglycans / Q000378:metabolism*; D006493:Heparin / Q000378:metabolism / Q000494:pharmacology; D006497:Heparan Sulfate / Q000378:metabolism; D008969:Molecular Sequence Data; D009132:Muscles / Q000166:cytology / Q000196:embryology* / Q000378:metabolism; D009154:Mutation; D017570:MyoD Protein / Q000235:genetics / Q000378:metabolism; D016376:Oligonucleotides, Antisense; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D011784:Quail / Q000196:embryology*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D016415:Sequence Alignment; D015398:Signal Transduction; D019170:Somites / Q000378:metabolism; D013234:Stem Cells / Q000378:metabolism*; D013429:Sulfatases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism; D014162:Transfection; D051153:Wnt Proteins; D029961:Zebrafish Proteins
chemical_list: D053823:Hedgehog Proteins; D019812:Heparan Sulfate Proteoglycans; D006493:Heparin; D006497:Heparan Sulfate; D017570:MyoD Protein; D016376:Oligonucleotides, Antisense; D011518:Proto-Oncogene Proteins; D011993:Recombinant Fusion Proteins; D013429:Sulfatases; D015534:Trans-Activators; D051153:Wnt Proteins; D029961:Zebrafish Proteins