Vasorelaxant effects of the bioflavonoid chrysin in isolated rat aorta.
Chrysin relaxed the contractions induced by noradrenaline in isolated endothelium-intact rat aortic rings (IC(50) = 16 +/- 4 microM). Endothelium removal and N(G)-nitro-L-arginine methyl ester inhibited this relaxant effect. Chrysin potentiated the relaxation to acetylcholine under control conditions or after incubation with the superoxide anion generator hypoxanthine/xanthine oxidase. It also potentiated the relaxation induced by 3-morpholino-sydnonimine, sodium nitroprusside, and 8-bromoguanosine-3':5'-cyclic-monophosphate. Therefore, vasorelaxation induced by chrysin in the rat aorta is endothelium- and NO-dependent. This effect is mediated by the prevention of O(2)(-)-induced inactivation of endothelial derived NO and also by the potentiation of cGMP-induced vasodilatation.
Duarte
J
J
Jiménez
R
R
Villar
I C
IC
Pérez-Vizcaíno
F
F
Jiménez
J
J
Tamargo
J
J
D000818:Animals; D001011:Aorta; D002352:Carrier Proteins / Q000378:metabolism; D004730:Endothelium, Vascular / Q000187:drug effects; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology*; D066298:In Vitro Techniques; D020128:Inhibitory Concentration 50; D047908:Intracellular Signaling Peptides and Proteins; D008297:Male; D009126:Muscle Relaxation / Q000187:drug effects*; D009131:Muscle, Smooth, Vascular / Q000187:drug effects*; D009569:Nitric Oxide / Q000378:metabolism; D051381:Rats; D014664:Vasodilation / Q000187:drug effects
D002352:Carrier Proteins; D005419:Flavonoids; D047908:Intracellular Signaling Peptides and Proteins; C022994:cyclic GMP-binding protein; D009569:Nitric Oxide; C043561:chrysin