title: Novel vectors for co-expression of two proteins in E. coli.
abstract: Two new vectors, pAC28 and pEGST, for the co-expression of recombinant genes in E. coli were developed. This two-plasmid system allows for an efficient expression and purification of large amounts of protein-protein complexes formed in bacterial cells. We have utilized this new system to express and isolate a stable complex of two human proteins, hematopoietic cell tyrosine phosphatase (HePTP) and mitogen-activated proteins kinase Erk2. This approach is useful for biochemical and structural studies of protein-protein interactions.
authors.affiliation: La Jolla Cancer Research Center, La Jolla, CA, USA.
authors.forename: N
authors.initials: N
authors.lastname: Kholod
authors.forename: T
authors.initials: T
authors.lastname: Mustelin
mesh_terms: D003001:Cloning, Molecular / Q000379:methods; D004926:Escherichia coli / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D005822:Genetic Vectors / Q000235:genetics*; D006801:Humans; D019950:Mitogen-Activated Protein Kinase 1 / Q000235:genetics; D010957:Plasmids / Q000235:genetics*; D017027:Protein Tyrosine Phosphatases / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D011994:Recombinant Proteins; D019950:Mitogen-Activated Protein Kinase 1; D017027:Protein Tyrosine Phosphatases