Structure-activity relationship studies of protective function of nitroxides in Fenton system.
The aim of this study was to evaluate the effect of piperidine nitroxides and their amine precursors on deoxyribose oxidation in the Fenton system. Protecting activity of nitroxides was found to be concentration-dependent and strongly influenced by ring substituents, while secondary amines did not provide any protection. The reported results suggest a mechanism of nitroxide action through iron oxidation rather than through direct scavenging of hydroxyl radicals. Moreover, presented data point to the danger of interference of nitroxides during the TBARS assay procedure.
Glebska
J
J
Department of Molecular Biophysics, University of Lodz, Poland.
Pulaski
L
L
Gwozdzinski
K
K
Skolimowski
J
J
D000588:Amines / Q000737:chemistry / Q000378:metabolism; D000975:Antioxidants / Q000737:chemistry / Q000378:metabolism; D003497:Cyclic N-Oxides / Q000737:chemistry / Q000378:metabolism; D003855:Deoxyribose / Q000737:chemistry* / Q000378:metabolism; D005609:Free Radicals / Q000737:chemistry / Q000378:metabolism; D006861:Hydrogen Peroxide; D007501:Iron; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D009589:Nitrogen Oxides / Q000737:chemistry / Q000378:metabolism*; D010084:Oxidation-Reduction; D020011:Protective Agents / Q000737:chemistry / Q000378:metabolism*; D013113:Spin Labels; D013329:Structure-Activity Relationship; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D000588:Amines; D000975:Antioxidants; D003497:Cyclic N-Oxides; C045076:Fenton's reagent; D005609:Free Radicals; D009589:Nitrogen Oxides; D020011:Protective Agents; D013113:Spin Labels; D017392:Thiobarbituric Acid Reactive Substances; D003855:Deoxyribose; D006861:Hydrogen Peroxide; D007501:Iron; D013482:Superoxide Dismutase; C039900:nitroxyl; C001803:tempol