title: Distribution of the SsuDAT1I restriction-modification system among different serotypes of Streptococcus suis.
abstract: The SsuDAT1I restriction-modification (R-M) system, which contains two methyltransferases and two restriction endonucleases with recognition sequence 5'-GATC-3', was first found in a field isolate of Streptococcus suis serotype 2. Isoschizomers of the R-M system were found in the same locus between purH and purD in a field isolate of serotype 1/2 and the reference strains of serotypes 3, 7, 23, and 26 among 29 strains of different serotypes examined in this study. The R-M gene sequences in serotypes 1/2, 3, 7, and 23 were very similar to those of SsuDAT1I, whereas those in serotype 26 were less similar. These results indicate intraspecies recombination among them and genetic divergence through their evolution.
authors.affiliation: National Institute of Animal Health, Tsukuba, Ibaraki, Japan. sekizaki@niah.affrc.go.jp
authors.forename: T
authors.initials: T
authors.lastname: Sekizaki
authors.forename: M
authors.initials: M
authors.lastname: Osaki
authors.forename: D
authors.initials: D
authors.lastname: Takamatsu
authors.forename: Y
authors.initials: Y
authors.lastname: Shimoji
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D004262:DNA Restriction Enzymes / Q000737:chemistry / Q000235:genetics; D015280:DNA Restriction-Modification Enzymes / Q000737:chemistry / Q000235:genetics*; D022761:Gene Transfer, Horizontal; D005798:Genes, Bacterial; D008780:Methyltransferases / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012703:Serotyping; D017011:Streptococcus suis / Q000145:classification* / Q000201:enzymology* / Q000235:genetics
chemical_list: D015280:DNA Restriction-Modification Enzymes; D008780:Methyltransferases; D004262:DNA Restriction Enzymes