Specification of bundle sheath cell fates during maize leaf development: roles of lineage and positional information evaluated through analysis of the tangled1 mutant.
In leaves of the maize tangled1 (tan1) mutant, clusters of bundle sheath (BS)-like cells extend several cells distant from the veins, in association with the single layer of BS cells around the vein. We show that the BS-like cell clusters in tan1 leaves result from the continued division of cells in the procambial/BS cell lineage that do not divide further in wild-type leaves. The ectopic BS-like cells accumulate the BS marker NADP-dependent malic enzyme but not the mesophyll cell marker phosphoenolpyruvate carboxylase, and exhibit thickened walls, suggesting that they differentiate as C4-type BS cells. We propose that bundle sheath cell fate can be conferred on some derivatives of procambial cell divisions in a manner that is heritable through multiple cell divisions and is position-independent.
Jankovsky
J P
JP
Department of Molecular, Cellular and Developmental Biology, Yale University, PO Box 208104, New Haven, CT 06520, USA.
Smith
L G
LG
Nelson
T
T
D002454:Cell Differentiation; D019070:Cell Lineage; D016296:Mutagenesis; D018515:Plant Leaves / Q000166:cytology / Q000254:growth & development; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D003313:Zea mays / Q000166:cytology* / Q000254:growth & development
D010940:Plant Proteins