title: Cholecystectomy prevents expansion of the bile acid pool and inhibition of cholesterol 7alpha-hydroxylase in rabbits fed cholesterol.
abstract: To study the effect of cholecystectomy on the regulation of classic and alternative bile acid syntheses, gallbladder-intact (n = 20) and cholecystectomized (n = 20) New Zealand White rabbits were fed either chow or chow with 2% cholesterol (3 g/day). After 10 days, bile fistulas were constructed in half of each rabbit group to recover and measure the bile acid pool and biliary bile acid flux. After cholesterol feeding, the bile acid pool size increased from 268 +/- 55 to 444 +/- 77 mg (P < 0.01) with a 2-fold rise in the biliary bile acid flux in intact rabbits but did not expand the bile acid pool (270 +/- 77 vs. 276 +/- 62 mg), nor did the biliary bile acid flux increase in cholecystectomized rabbits. Ileal apical sodium-dependent bile acid transporter protein increased 46% from 93 +/- 6 to 136 +/- 23 units/mg (P < 0.01) in the intact rabbits but did not change in cholecystectomized rabbits (104 +/- 14 vs. 99 +/- 19 units/mg) after cholesterol feeding. Cholesterol 7alpha-hydroxylase activity was inhibited 59% (P < 0.001) while cholesterol 27-hydroxylase activity rose 83% (P < 0.05) after cholesterol feeding in the intact rabbits but neither enzyme activity changed significantly in cholesterol-fed cholecystectomized rabbits. Fecal bile acid outputs reflecting bile acid synthesis increased significantly in the intact but not in the cholecystectomized rabbits fed cholesterol. Removal of the gallbladder prevented expansion of the bile acid pool after cholesterol feeding as seen in intact rabbits because ileal bile acid transport did not increase. As a result, cholesterol 7alpha-hydroxylase was not inhibited.
authors.affiliation: Medical Service, Veterans Affairs Medical Center, East Orange, NJ 07018-1095, USA.
authors.forename: G
authors.initials: G
authors.lastname: Xu
authors.forename: G
authors.initials: G
authors.lastname: Salen
authors.forename: B L
authors.initials: BL
authors.lastname: Shneider
authors.forename: M
authors.initials: M
authors.lastname: Ananthanarayanan
authors.forename: S
authors.initials: S
authors.lastname: Shefer
authors.forename: L
authors.initials: L
authors.lastname: Ma
authors.forename: A
authors.initials: A
authors.lastname: Batta
authors.forename: L B
authors.initials: LB
authors.lastname: Nguyen
authors.forename: J J
authors.initials: JJ
authors.lastname: Lingutla
authors.forename: G S
authors.initials: GS
authors.lastname: Tint
authors.forename: M
authors.initials: M
authors.lastname: Pcolinsky
authors.forename: F J
authors.initials: FJ
authors.lastname: Suchy
mesh_terms: D000818:Animals; D001647:Bile Acids and Salts / Q000096:biosynthesis* / Q000378:metabolism; D015153:Blotting, Western; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D002763:Cholecystectomy; D053493:Cholestanetriol 26-Monooxygenase; D002784:Cholesterol / Q000097:blood / Q000378:metabolism; D002790:Cholesterol 7-alpha-Hydroxylase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D002791:Cholesterol, Dietary / Q000008:administration & dosage*; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D005243:Feces / Q000737:chemistry; D005704:Gallbladder / Q000502:physiology*; D006903:Hydroxymethylglutaryl CoA Reductases / Q000378:metabolism; D006913:Hydroxysteroid Dehydrogenases; D007082:Ileum / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D029363:Organic Anion Transporters, Sodium-Dependent; D012333:RNA, Messenger / Q000032:analysis; D011817:Rabbits; D013250:Steroid Hydroxylases / Q000378:metabolism; D027981:Symporters; D045002:3-alpha-Hydroxysteroid Dehydrogenase (B-Specific)
chemical_list: D001647:Bile Acids and Salts; D002352:Carrier Proteins; D053493:Cholestanetriol 26-Monooxygenase; D002784:Cholesterol; D002790:Cholesterol 7-alpha-Hydroxylase; D002791:Cholesterol, Dietary; D003577:Cytochrome P-450 Enzyme System; D006903:Hydroxymethylglutaryl CoA Reductases; D006913:Hydroxysteroid Dehydrogenases; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D029363:Organic Anion Transporters, Sodium-Dependent; D012333:RNA, Messenger; D013250:Steroid Hydroxylases; D027981:Symporters; C037643:bile acid binding proteins; C071908:sodium-bile acid cotransporter; C435496:AKR1C2 protein, human; D045002:3-alpha-Hydroxysteroid Dehydrogenase (B-Specific); C506831:CYP27A1 protein, human