Determination of mevalonic acid in human urine as mevalonic acid lactone by gas chromatography-mass spectrometry.
Mevalonic acid in urine is converted to its lactone form by incubation overnight at acidic pH, extracted and analysed by GC-MS. The lower limit of quantitation of 7.5 ng/ml provides adequate sensitivity to measure changes in urinary excretion of MVA following administration of drugs which affect cholesterol synthesis. The assay is linear up to 300 ng/ml and has acceptable precision (<15%) and accuracy (<+/-20%) across the calibration range.
Woollen
B H
BH
Syngenta CTL, Alderley Park, Nr. Macclesfield, Cheshire, UK. Bruce.woollen@syngenta.com
Holme
P C
PC
Northway
W J
WJ
Martin
P D
PD
D002138:Calibration; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D007783:Lactones / Q000652:urine*; D008798:Mevalonic Acid / Q000652:urine*; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D007783:Lactones; D008798:Mevalonic Acid