Gas-phase conformers of the [M + 2H](2+) ion of bradykinin investigated by combining high-field asymmetric waveform ion mobility spectrometry, hydrogen/deuterium exchange, and energy-loss measurements.
The continuous separation capability of high-field asymmetric waveform ion mobility spectrometry (FAIMS) was used in combination with complementary techniques for probing biomolecular ions in the gas phase. Gas-phase conformers of the [M + 2H](2+) ion of bradykinin were examined using a combination of FAIMS, H/D exchange, and energy-loss measurements. When FAIMS data and H/D exchange data were analyzed separately, the presence of only two conformers of the [M + 2H](2+) ion of bradykinin could be detected. However, in an experiment in which FAIMS and H/D exchange were combined, at least four different conformers of the gas-phase [M + 2H](2+) ion of bradykinin were detected, including one of very low abundance. Cross sections calculated for the four conformers, based on energy-loss measurements, were 250, 240, 250, and 244 A(2), in order of decreasing abundance.
Purves
R W
RW
Institute for National Measurement Standards, National Research Council of Canada, Ottawa, K1A 0R6, Canada.
Barnett
D A
DA
Ells
B
B
Guevremont
R
R
D001920:Bradykinin / Q000737:chemistry*; D005740:Gases; D013058:Mass Spectrometry / Q000379:methods; D011487:Protein Conformation; D013816:Thermodynamics
D005740:Gases; D001920:Bradykinin