Aromatase in synovial cells from postmenopausal women.
Peripheral aromatization of androgens exerts estrogenic actions in many tissues. In this study, osteoarthritis synoviocytes were examined to clarify the possible action of adrenal androgen on synovial cell. Synoviocytes from postmenopausal women are able to express aromatase mRNA. By sequence analysis, the PCR fragment (485 bp) was determined to be 100% identical to that of human placental aromatase cDNA. Moreover, this study demonstrates that adrenal androgen, androstenedione, is converted to estrone (E(1)) and estradiol (E(2)) in synoviocytes by aromatase which is positively regulated by glucocorticoids such as dexamethasone. E(2) production reduced significantly IL-6 secretion. These data provide preliminary evidence that in situ estrogen production by synoviocytes may have a role in OA susceptibility. However the role of E(2) in OA is not clear and remains to be determined.
Le Bail
J
J
Sanofi-Synthelabo, Target Identification, Internal Medicine, Rueil-Malmaison, Limoges, France.
Liagre
B
B
Vergne
P
P
Bertin
P
P
Beneytout
J
J
Habrioux
G
G
D000735:Androstenedione / Q000737:chemistry / Q000378:metabolism; D001141:Aromatase / Q000235:genetics / Q000378:metabolism*; D003994:Bucladesine / Q000494:pharmacology; D002478:Cells, Cultured; D017077:Culture Media, Conditioned; D003907:Dexamethasone / Q000494:pharmacology; D015232:Dinoprostone / Q000378:metabolism; D004967:Estrogens / Q000378:metabolism; D005260:Female; D005938:Glucocorticoids / Q000494:pharmacology; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism; D010003:Osteoarthritis / Q000378:metabolism; D017698:Postmenopause; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013583:Synovial Membrane / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*; D014316:Tritium / Q000378:metabolism
D017077:Culture Media, Conditioned; D004967:Estrogens; D005938:Glucocorticoids; D015850:Interleukin-6; D014316:Tritium; D000735:Androstenedione; D003994:Bucladesine; D003907:Dexamethasone; D001141:Aromatase; D015232:Dinoprostone