title: Polymorphic microsatellites developed by cross-species amplifications in common pheasant breeds.
abstract: Genetic variability was analysed in two common breeds of pheasant (Phasianus colchicus L. 1758) by means of cross-species amplifications of microsatellite loci: 154 chicken, Gallus gallus and 32 turkey, Meleagris gallopavo, primers were tested for amplification of pheasant DNA. Thirty-six primers (25 specific for chicken and 11 for turkey) amplified pheasant DNA. Fifteen markers yielded specific products and were tested for polymorphism. Eight of them (55%) were polymorphic, with an average polymorphism of two alleles per locus. Specific polymerase chain reaction (PCR) products were sequenced; repeats were found in 11 of the 15 markers, although only two loci showed the same repeat and could be homologous to chicken ones.
authors.affiliation: Department of Animal Biology and Genetics, University of Florence, Florence, Italy. baratti@fi.cnr.it
authors.forename: M
authors.initials: M
authors.lastname: Baratti
authors.forename: A
authors.initials: A
authors.lastname: Alberti
authors.forename: M
authors.initials: M
authors.lastname: Groenen
authors.forename: T
authors.initials: T
authors.lastname: Veenendaal
authors.forename: F D
authors.initials: FD
authors.lastname: Fulgheri
mesh_terms: D000483:Alleles; D000818:Animals; D001717:Birds / Q000235:genetics*; D002645:Chickens; D004247:DNA / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D014644:Genetic Variation / Q000235:genetics; D018895:Microsatellite Repeats / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA; D014421:Turkey
chemical_list: D004247:DNA