Procalcitonin and CGRP-1 mrna expression in various human tissues.
Procalcitonin (PCT) is a highly sensitive and specific marker of systemic bacterial infection and sepsis. In contrast to its diagnostic significance, the cellular sources of plasma procalcitonin remain to be clarified. Two forms of PCT mRNAs originate from calcitonin/calcitonin gene-related peptide gene (CALC-I gene) along with mRNA for calcitonin gene-related peptide-I (CGRP-I). Reverse transcription polymerase chain reaction with newly designed primers detecting different PCT mRNAs and CGRP-I mRNA was used to identify tissues that might contribute to PCT production. Our study indicates that a variety of human tissues (13 of the 16 analyzed overall) express PCT-I, PCT-II, and/or CGRP-I mRNAs, with the highest levels detected for liver, testis, lung, prostate, kidney, and small intestine. Various tissues differ in the proportions of PCT-I, PCT-II, and CGRP-I mRNA expression levels. Thus we demonstrate the complexity of tissue-specific regulation of CALC-I gene expression and suppose a variety of tissues as a potential source of CALC-I-encoded peptides.
Russwurm
S
S
Department of Anesthesiology and Critical Care Medicine, Friedrich-Schiller-University of Jena, Germany.
Stonans
I
I
Stonane
E
E
Wiederhold
M
M
Luber
A
A
Zipfel
P F
PF
Deigner
H P
HP
Reinhart
K
K
D002116:Calcitonin / Q000097:blood / Q000235:genetics*; D015740:Calcitonin Gene-Related Peptide / Q000097:blood / Q000235:genetics*; D017931:DNA Primers; D018076:DNA, Complementary; D015870:Gene Expression; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000502:physiology; D009928:Organ Specificity; D016133:Polymerase Chain Reaction; D011498:Protein Precursors / Q000097:blood / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*; D015183:Restriction Mapping; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
C000606589:CALCA protein, human; D017931:DNA Primers; D018076:DNA, Complementary; D011498:Protein Precursors; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D002116:Calcitonin; D015740:Calcitonin Gene-Related Peptide