title: Separation of mistletoe lectins based on the degree of glycosylation using boronate affinity chromatography.
abstract: A mixture of two mistletoe lectins (MLs) has been separated according to the degree of glycosylation using boronate affinity chromatography. The mistletoe lectins, mistletoe lectin I (MLI) and mistletoe lectin III (MLIII) with degrees of glycosylation of 6.1 and 3.8%, respectively, were used in the investigation. MLI exhibited a higher retention time than MLIII due to its higher degree of glycosylation. Separation was confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The developed method may lead to new applications for the boronate affinity technique, as well as provide an alternative separation method for MLs.
authors.affiliation: Gramineer International AB, IDEON, Lund, Sweden.
authors.forename: Y
authors.initials: Y
authors.lastname: Li
authors.forename: U
authors.initials: U
authors.lastname: Pfüller
authors.forename: E L
authors.initials: EL
authors.lastname: Larsson
authors.forename: H
authors.initials: H
authors.lastname: Jungvid
authors.lastname: Galaev IYu
authors.forename: B
authors.initials: B
authors.lastname: Mattiasson
mesh_terms: D001897:Boronic Acids / Q000737:chemistry*; D002846:Chromatography, Affinity / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D006031:Glycosylation; D028321:Plant Preparations; D010940:Plant Proteins; D054790:Ribosome Inactivating Proteins, Type 2; D012685:Sepharose; D014118:Toxins, Biological / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D001897:Boronic Acids; D028321:Plant Preparations; D010940:Plant Proteins; D054790:Ribosome Inactivating Proteins, Type 2; D014118:Toxins, Biological; C009674:ribosome inactivating protein, Viscum; D012685:Sepharose