title: GABA(A) receptor cell surface number and subunit stability are regulated by the ubiquitin-like protein Plic-1.
abstract: Controlling the number of functional gamma-aminobutyric acid A (GABA(A)) receptors in neuronal membranes is a crucial factor for the efficacy of inhibitory neurotransmission. Here we describe the direct interaction of GABA(A) receptors with the ubiquitin-like protein Plic-1. Furthermore, Plic-1 is enriched at inhibitory synapses and is associated with subsynaptic membranes. Functionally, Plic-1 facilitates GABA(A) receptor cell surface expression without affecting the rate of receptor internalization. Plic-1 also enhances the stability of intracellular GABA(A) receptor subunits, increasing the number of receptors available for insertion into the plasma membrane. Our study identifies a previously unknown role for Plic-1, a modulation of GABA(A) receptor cell surface number, which suggests that Plic-1 facilitates accumulation of these receptors in dendritic membranes.
authors.affiliation: Medical Research Council Laboratory of Molecular Cell Biology and Department of Pharmacology, University College London, Gower Street, London WC1E 6BT, UK.
authors.forename: F K
authors.initials: FK
authors.lastname: Bedford
authors.forename: J T
authors.initials: JT
authors.lastname: Kittler
authors.forename: E
authors.initials: E
authors.lastname: Muller
authors.forename: P
authors.initials: P
authors.lastname: Thomas
authors.forename: J M
authors.initials: JM
authors.lastname: Uren
authors.forename: D
authors.initials: D
authors.lastname: Merlo
authors.forename: W
authors.initials: W
authors.lastname: Wisden
authors.forename: A
authors.initials: A
authors.lastname: Triller
authors.forename: T G
authors.initials: TG
authors.lastname: Smart
authors.forename: S J
authors.initials: SJ
authors.lastname: Moss
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D000071183:Autophagy-Related Proteins; D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D002462:Cell Membrane / Q000378:metabolism; D004355:Drug Stability; D020033:Protein Isoforms / Q000378:metabolism; D051381:Rats; D011963:Receptors, GABA-A / Q000378:metabolism*; D013347:Subcellular Fractions / Q000378:metabolism; D014018:Tissue Distribution; D014452:Ubiquitins / Q000378:metabolism / Q000502:physiology*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D000071183:Autophagy-Related Proteins; D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D020033:Protein Isoforms; D011963:Receptors, GABA-A; C107792:UBQLN1 protein, human; D014452:Ubiquitins