Isolation and characterization of Achromobacter xylosoxidans T7 capable of degrading toluidine isomers.
A bacterial strain capable of utilizing toluidine isomers as its sole source of carbon and energy for growth was isolated from contaminated soil. The isolate was identified as Achromobacter xylosoxidans and was designated strain T7. Strain T7 differs from other toluidine-degrading strains with respect to the use of all three toluidine isomers even as an equimolar mixture. Additionally, strain T7 harbours the ability to use aniline, phenol, and cresols as growth substrates. Utilization of the toluidine isomers was demonstrated by an increase in the bacterial biomass concomitant with a decrease of the respective toluidine concentration in liquid medium with this compound as sole source of carbon and energy. No accumulation of any intermediate was detectable by HPLC-analyses. Results of oxygen uptake experiments with resting cells of strain T7 pre-grown on the respective toluidine and enzymatic investigations in cell-free extracts indicate the metabolization of the toluidines via the respective methylcatechols as intermediates. These compounds are substrates for the meta-cleavage pathway initiated by inducible catechol 2,3-dioxygenase found in toluidine-grown cells of strain T7.
Hinteregger
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C
Section of Environmental Microbiology, Institute of Biochemical Technology and Microbiology, University of Technology Vienna, Getreidemarkt 9, A-1060 Vienna, Austria.
Streichsbier
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F
D020562:Betaproteobacteria / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism; D001673:Biodegradation, Environmental; D050548:Catechol 1,2-Dioxygenase; D050557:Catechol 2,3-Dioxygenase; D003470:Culture Media; D049308:Dioxygenases; D007700:Kinetics; D010100:Oxygen; D010105:Oxygenases; D013379:Substrate Specificity; D014052:Toluidines / Q000378:metabolism*
D003470:Culture Media; D014052:Toluidines; D010105:Oxygenases; D049308:Dioxygenases; D050548:Catechol 1,2-Dioxygenase; D050557:Catechol 2,3-Dioxygenase; D010100:Oxygen