title: [Visceral leishmaniasis in immunocompetent children: diagnostic and epidemiologic value of peripheral leishmania blood culture].
abstract: Utilisation of new diagnosis means and particularly non invasive oues in visceral leishmaniasis can be very valuable for the biologist, the clinician as well as the patient. In this, detection of leishmania in peripheral blood, well know for VIH patients, has been applied to 37 immunocompetent tunisan children suffering from kala azar that has been shown through direct examination of bone marrow. Observed results show that culture on NNN peripheral blood medium was positive in 25 cases (67.57%). On the other side, detection of leishmania through concomitant culture of blood and marrow bone for 24 children with visceral leishmaniasis match the results in 75% of the cases. Detection of leishmania by mean of blood culture for immunocompetent children is a diagnosis mean of visceral leishmaniasis and has also an epidemiologic utility by isoenzymatic characterization of isolated leishmania strains.
authors.affiliation: Laboratoire de Parasitologie, hôpital la Rabta, Tunisie.
authors.forename: S
authors.initials: S
authors.lastname: Belhadj
authors.forename: N
authors.initials: N
authors.lastname: el Houda Toumi
authors.forename: K
authors.initials: K
authors.lastname: Kallel
authors.forename: H
authors.initials: H
authors.lastname: Dakhlia
authors.forename: F
authors.initials: F
authors.lastname: Pratlong
authors.forename: N
authors.initials: N
authors.lastname: Boussen
authors.forename: S
authors.initials: S
authors.lastname: Bousnina
authors.forename: H
authors.initials: H
authors.lastname: Babba
authors.forename: S
authors.initials: S
authors.lastname: Ben Becher
authors.forename: R
authors.initials: R
authors.lastname: Azaiez
authors.forename: F
authors.initials: F
authors.lastname: Khaldi
authors.forename: E
authors.initials: E
authors.lastname: Chaker
mesh_terms: D000818:Animals; D001769:Blood / Q000469:parasitology*; D001856:Bone Marrow Examination / Q000592:standards; D002648:Child; D003470:Culture Media; D006801:Humans; D007121:Immunocompetence; D007891:Leishmania / Q000254:growth & development* / Q000302:isolation & purification*; D007898:Leishmaniasis, Visceral / Q000097:blood / Q000175:diagnosis* / Q000453:epidemiology* / Q000276:immunology / Q000469:parasitology; D010274:Parasitology / Q000379:methods* / Q000592:standards; D011159:Population Surveillance; D011446:Prospective Studies; D014416:Tunisia / Q000453:epidemiology
chemical_list: D003470:Culture Media
vernacular_title: La leishmaniose viscérale, de l'enfant immunocompétent: intérêt diagnostique et épidémiologique de la recherche des leishmanies par culture du sang périphérique.