[Genetic control of growth and development of yeast Saccharomyces cerevisiae cells. Phenotypic selection of mutants among strains of the Peterhof genetic collection].
Results of identifying phenotypes intrinsic to mutations of genes that regulate the activity of the signal transduction pathway of RAS-cyclic adenosinemonophosphate in six strains belonging to the Peterhof genetic collection of the yeast Saccharomyces cerevisiae are presented: an increase or decrease in the amount of the accumulated glycogen, resistance or sensitivity to heat shock and nitrogen starvation, and the growth and viability in media containing unfermentable carbon sources (potassium acetate, ethanol, and glycerol) at temperatures 30 and 70 degrees C. Collectively these phenotypic characteristics in five examined yeast strains can be interpreted as indicating disturbances in the activity of the RAS/cAMP pathway. However, the discrepancies revealed between cellular phenotypes in these strains and in the strains with a decreased or increased activity of the RAS/cAMP pathway did not allow them to be assigned to a particular functional activity group. The inconsistencies between phenotypes detected in this study may be prerequisites for the identification of new genes responsible for this signal transduction pathway or new mutations in the known genes that determine other phenotypic combinations.
Chitavichius
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Department of Microbiology and Plant Physiology, Vilnius University, Vilnius, 2009 Lithuania. citavicius@yahoo.com
D000242:Cyclic AMP; D000431:Ethanol / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D011905:Genes, ras; D005990:Glycerol / Q000378:metabolism; D006003:Glycogen / Q000378:metabolism; D009154:Mutation; D009584:Nitrogen / Q000378:metabolism; D010641:Phenotype; D019347:Potassium Acetate / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000145:classification / Q000235:genetics* / Q000254:growth & development*; D015398:Signal Transduction; D013696:Temperature
D000431:Ethanol; D006003:Glycogen; D000242:Cyclic AMP; D019347:Potassium Acetate; D009584:Nitrogen; D005990:Glycerol
Geneticheskiĭ kontrol' rosta i razvitiia kletok drozhzheĭ Saccharomyces cerevisiae. Fenotipicheskiĭ otbor mutantov sredi shtammov Petergofskoĭ geneticheskoi kollektsii.