Neurophilin-1 is a downstream target of transcription factor Ets-1 in human umbilical vein endothelial cells.
Transcription factor Ets-1 expressed in endothelial cells promotes angiogenesis. Here, we transiently overexpressed Ets-1 in human umbilical vein endothelial cells (HUVECs) and comprehensively searched for potential downstream targets of Ets-1 by cDNA microarray analysis. The expression of several angiogenesis-related genes including neuropilin-1 was augmented by the overexpression of Ets-1. Quantitative real-time RT-PCR and Western blotting confirmed the increase in the levels of neuropilin-1 mRNA and protein. In contrast, dominant negative ets-1 decreased the levels of neuropilin-1 mRNA and protein. These results indicate that neuropilin-1 is a downstream target of Ets-1 in HUVECs.
Teruyama
K
K
Department of Vascular Biology, Institute of Development, Aging and Cancer, Tohoku University, Sendai, Japan.
Abe
M
M
Nakano
T
T
Takahashi
S
S
Yamada
S
S
Sato
Y
Y
D001483:Base Sequence; D002478:Cells, Cultured; D017931:DNA Primers; D018076:DNA, Complementary; D016228:Endothelial Growth Factors / Q000235:genetics / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D008222:Lymphokines / Q000235:genetics / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D011485:Protein Binding; D050784:Proto-Oncogene Protein c-ets-1; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D050783:Proto-Oncogene Proteins c-ets; D014157:Transcription Factors / Q000378:metabolism*; D014471:Umbilical Veins / Q000166:cytology / Q000378:metabolism*; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
D017931:DNA Primers; D018076:DNA, Complementary; C494041:ETS1 protein, human; D016228:Endothelial Growth Factors; D008222:Lymphokines; D050784:Proto-Oncogene Protein c-ets-1; D011518:Proto-Oncogene Proteins; D050783:Proto-Oncogene Proteins c-ets; D014157:Transcription Factors; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors