Prostaglandin E(2) increases surfactant secretion via the EP(1) receptor in rat alveolar type II cells.
Prostaglandin E(2), the predominant cyclooxygenase metabolite of arachidonic acid in alveolar type II cells, can stimulate pulmonary surfactant secretion. The actions of prostaglandin E(2) are mediated by four prostaglandin E (EP) receptor subtypes designated EP(1), EP(2), EP(3) and EP(4). These subtypes couple to different signal transduction pathways. However, it is not clear which of these subtypes is expressed on type II cells and mediates surfactant secretion. We found that the four subtypes of EP receptors are expressed on the primary cultured alveolar type II cells from adult rats. We also concluded that EP(1) receptor appears to mediate prostaglandin E(2)-induced surfactant secretion through Ca(2+) mobilization.
Morsy
M A
MA
Department of Pharmacological Sciences, Faculty of Pharmaceutical Sciences, Kumamoto University, 5-1 Oe-honmachi, 862-0973, Kumamoto, Japan.
Isohama
Y
Y
Miyata
T
T
D000818:Animals; D001643:Bridged Bicyclo Compounds / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D002208:Caproates / Q000494:pharmacology; D015232:Dinoprostone / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005786:Gene Expression Regulation / Q000187:drug effects; D008297:Male; D011650:Pulmonary Alveoli / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D011982:Receptors, Prostaglandin / Q000235:genetics; D018078:Receptors, Prostaglandin E / Q000235:genetics / Q000502:physiology*; D058306:Receptors, Prostaglandin E, EP1 Subtype; D058307:Receptors, Prostaglandin E, EP2 Subtype; D058309:Receptors, Prostaglandin E, EP4 Subtype; D013047:Specific Pathogen-Free Organisms; D013501:Surface-Active Agents / Q000378:metabolism*; D013997:Time Factors
D001643:Bridged Bicyclo Compounds; D002208:Caproates; C428179:EP3C receptor; C122148:ONO 8711; C545637:Ptger1 protein, rat; C545646:Ptger2 protein, rat; C545652:Ptger4 protein, rat; D012333:RNA, Messenger; D011982:Receptors, Prostaglandin; D018078:Receptors, Prostaglandin E; D058306:Receptors, Prostaglandin E, EP1 Subtype; D058307:Receptors, Prostaglandin E, EP2 Subtype; D058309:Receptors, Prostaglandin E, EP4 Subtype; D013501:Surface-Active Agents; D015232:Dinoprostone; D002118:Calcium