title: Telencephalic neural progenitors appear to be restricted to regional and glial fates before the onset of neurogenesis.
abstract: The contribution of early cell lineage to regional fate in the mammalian forebrain remains poorly understood. Previous lineage-tracing studies using retroviral methods were only begun at mid-neurogenesis and have suffered from region-specific retroviral silencing. We have been able to study cell lineage in the telencephalon from the onset of neurogenesis by using ultrasound backscatter microscopy to label the forebrain neuroepithelium and a modified retroviral lineage library to overcome regional silencing. Our studies suggest that by embryonic day 9.5, forebrain clones are primarily restricted to territories within anatomically demarcated regional boundaries, such as the cortex, striatum and hypothalamus. In addition, we observed a subset of clones that appeared to be composed entirely of glia. These observations suggest that both regional and cell-type restrictions exist within progenitor populations before the first forebrain cells become postmitotic.
authors.affiliation: Developmental Genetics Program and the Department of Cell Biology, and Molecular Neuroscience Program, The Skirball Institute of Biomolecular Medicine, New York University Medical Center, New York, New York 10016, USA.
authors.forename: M
authors.initials: M
authors.lastname: McCarthy
authors.forename: D H
authors.initials: DH
authors.lastname: Turnbull
authors.forename: C A
authors.initials: CA
authors.lastname: Walsh
authors.forename: G
authors.initials: G
authors.lastname: Fishell
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D019070:Cell Lineage / Q000502:physiology; D002465:Cell Movement / Q000502:physiology; D002999:Clone Cells / Q000166:cytology; D015723:Gene Library; D020868:Gene Silencing; D051379:Mice; D008853:Microscopy / Q000295:instrumentation; D009024:Morphogenesis; D009457:Neuroglia / Q000166:cytology* / Q000821:virology; D009474:Neurons / Q000166:cytology* / Q000821:virology; D012190:Retroviridae / Q000502:physiology; D013234:Stem Cells / Q000166:cytology* / Q000821:virology; D013687:Telencephalon / Q000166:cytology* / Q000196:embryology* / Q000821:virology; D014463:Ultrasonography / Q000295:instrumentation