title: Functionalized xenon as a biosensor.
abstract: The detection of biological molecules and their interactions is a significant component of modern biomedical research. In current biosensor technologies, simultaneous detection is limited to a small number of analytes by the spectral overlap of their signals. We have developed an NMR-based xenon biosensor that capitalizes on the enhanced signal-to-noise, spectral simplicity, and chemical-shift sensitivity of laser-polarized xenon to detect specific biomolecules at the level of tens of nanomoles. We present results using xenon "functionalized" by a biotin-modified supramolecular cage to detect biotin-avidin binding. This biosensor methodology can be extended to a multiplexing assay for multiple analytes.
authors.affiliation: Department of Chemistry, University of California, Berkeley, CA 94720, USA.
authors.forename: M M
authors.initials: MM
authors.lastname: Spence
authors.forename: S M
authors.initials: SM
authors.lastname: Rubin
authors.forename: I E
authors.initials: IE
authors.lastname: Dimitrov
authors.forename: E J
authors.initials: EJ
authors.lastname: Ruiz
authors.forename: D E
authors.initials: DE
authors.lastname: Wemmer
authors.forename: A
authors.initials: A
authors.lastname: Pines
authors.forename: S Q
authors.initials: SQ
authors.lastname: Yao
authors.forename: F
authors.initials: F
authors.lastname: Tian
authors.forename: P G
authors.initials: PG
authors.lastname: Schultz
mesh_terms: D001360:Avidin / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D001710:Biotin / Q000737:chemistry; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D011506:Proteins / Q000737:chemistry*; D014978:Xenon
chemical_list: D011506:Proteins; D001360:Avidin; D014978:Xenon; D001710:Biotin