Efficient biochemical engineering of cellular sialic acids using an unphysiological sialic acid precursor in cells lacking UDP-N-acetylglucosamine 2-epimerase.
Sialic acids comprise a family of terminal sugars essential for a variety of biological recognition systems. N-Propanoylmannosamine, an unphysiological sialic acid precursor, is taken up and metabolized by mammalian cells resulting in oligosaccharide-bound N-propanoylneuraminic acid. N-Propanoylmannosamine, applied to endogenously hyposialylated subclones of the myeloid leukemia HL60 and of the B-cell lymphoma BJA-B, both deficient in UDP-N-acetylglucosamine 2-epimerase, is efficiently metabolized to CMP-N-propanoylneuraminic acid resulting in up to 85% of glycoconjugate-associated sialic acids being unphysiological N-propanoylneuraminic acid. Thus, UDP-N-acetylglucosamine 2-epimerase-deficient cell lines provide an important experimental progress in engineering cells to display an almost homogeneous population of defined, structurally altered sialic acids.
Mantey
L R
LR
Institut für Molekularbiologie und Biochemie, Freie Universität Berlin, Germany.
Keppler
O T
OT
Pawlita
M
M
Reutter
W
W
Hinderlich
S
S
D002238:Carbohydrate Epimerases / Q000378:metabolism*; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D003569:Cytidine Monophosphate N-Acetylneuraminic Acid / Q000737:chemistry / Q000378:metabolism*; D029968:Escherichia coli Proteins; D005434:Flow Cytometry; D006801:Humans; D009438:Neuraminic Acids / Q000378:metabolism; D014407:Tumor Cells, Cultured
D029968:Escherichia coli Proteins; C425503:N-propanoylneuraminic acid; D009438:Neuraminic Acids; D003569:Cytidine Monophosphate N-Acetylneuraminic Acid; D002238:Carbohydrate Epimerases; C100033:UDP acetylglucosamine-2-epimerase; C083982:wecB protein, E coli