title: Rapid genotyping of human platelet antigen 5 with fluorophore-labelled hybridization probes on the LightCycler.
abstract: Genotyping of human platelet antigens (HPAs) can be useful for the diagnosis and therapy of alloimmune thrombocytopenic syndromes such as neonatal alloimmune thrombocytopenic purpura, post-transfusion purpura and refractoriness to platelet transfusion therapy. We developed a single-tube method for HPA-5 genotyping on the LightCycler that combines rapid-cycle polymerase chain reaction with allele-specific fluorescent probe melting for mutation detection. Our method is fast, robust and suitable for routine HPA-5 typing. This work extends recent studies on HPA-1 typing using the LightCycler.
authors.affiliation: Institute for Transfusion Medicine, Duisburg, Germany. ek@bzd.de
authors.forename: E
authors.initials: E
authors.lastname: Kretzschmar
authors.forename: N
authors.initials: N
authors.lastname: Von Ahsen
authors.forename: H
authors.initials: H
authors.lastname: Trobisch
mesh_terms: D016824:Antigens, Human Platelet / Q000235:genetics*; D001483:Base Sequence; D015342:DNA Probes / Q000235:genetics; D005838:Genotype; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000295:instrumentation; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D013921:Thrombocytopenia / Q000235:genetics*
chemical_list: C071092:5a alloantigen, human; D016824:Antigens, Human Platelet; D015342:DNA Probes