title: Microbiology and risk factors for catheter exit-site and -hub colonization in neonatal intensive care unit patients.
abstract: OBJECTIVE
  To identify risk factors and describe the microbiology of catheter exit-site and hub colonization in neonates.
  
  
  METHODS
  During a period of 2 years, we prospectively investigated 14 risk factors for catheter exit-site and hub colonization in 862 central venous catheters in a cohort of 441 neonates. Cultures of the catheter exit-site and hub were obtained using semiquantitative techniques at time of catheter removal.
  
  
  METHODS
  A neonatal intensive care unit at a university hospital.
  
  
  RESULTS
  Catheter exit-site colonization was found in 7.2% and hub colonization in 5.3%. Coagulase-negative staphylococci were predominant at both sites. Pathogenic flora were found more frequently at the catheter hub (36% vs 14%; P<.05). Through logistic regression, factors associated with exit-site colonization were identified as umbilical insertion (odds ratio [OR], 8.1; 95% confidence interval [CI95], 2.35-27.6; P<.001), subclavian insertion (OR, 54.6; CI95, 12.2-244, P<.001), and colonization of the catheter hub (OR, 8.9; CI, 3.5-22.8; P<.001). Catheter-hub colonization was associated with total parenteral nutrition ([TPN] OR for each day of TPN, 1.056; CI95, 1.029-1.083; P<.001) and catheter exit-site colonization (OR, 6.11; CI95, 2.603-14.34; P<.001). No association was found between colonization at these sites and duration of catheterization and venue of insertion, physician's experience, postnatal age and patient's weight, ventilation, steroids or antibiotics, and catheter repositioning.
  
  
  CONCLUSIONS
  These data support that colonization of the catheter exit-site is associated with the site of insertion and colonization of the catheter hub with the use of TPN. There is a very strong association between colonization at both catheter sites.
authors.affiliation: Department of Pediatrics, University Hospital of Antwerp, Belgium.
authors.forename: L M
authors.initials: LM
authors.lastname: Mahieu
authors.forename: J J
authors.initials: JJ
authors.lastname: De Dooy
authors.forename: A O
authors.initials: AO
authors.lastname: De Muynck
authors.forename: G
authors.initials: G
authors.lastname: Van Melckebeke
authors.forename: M M
authors.initials: MM
authors.lastname: Ieven
authors.forename: P J
authors.initials: PJ
authors.lastname: Van Reempts
mesh_terms: D000704:Analysis of Variance; D001530:Belgium; D002405:Catheterization, Central Venous / Q000009:adverse effects*; D002408:Catheters, Indwelling / Q000382:microbiology*; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007363:Intensive Care Units, Neonatal; D008297:Male; D011446:Prospective Studies; D012307:Risk Factors