Sequence patterns defining the 5' boundary of human genes.
In studies of both short and relatively long human genomic DNA, we found a clustering of the consensus site for the transcription factor GCF at the 5' boundary of a subset of human genes. In studies of promoter regions with known transcription initiation site, the cluster of consensus GCF site appeared near the transcription initiation site and in some sequences it extended into the transcribed region defining the leader mRNA. We also found a detectable correlation between the 5' boundary of human genes and recognition motifs for other transcription factors that bind to GC-rich sequences. But in these cases, the correlation was not as general as the correlation observed for the consensus GCF site.
Bina
M
M
Department of Chemistry, Purdue University, W. Lafayette, IN 47907-1393, USA. Bina@Purdue.edu
Crowely
E
E
D020121:5' Untranslated Regions / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D001665:Binding Sites; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D005091:Exons; D020224:Expressed Sequence Tags; D005796:Genes; D015894:Genome, Human; D006801:Humans; D005810:Multigene Family; D011401:Promoter Regions, Genetic; D011544:Pseudogenes; D017422:Sequence Analysis, DNA / Q000379:methods; D012984:Software; D016385:TATA Box; D014157:Transcription Factors / Q000378:metabolism
D020121:5' Untranslated Regions; D014157:Transcription Factors; D004247:DNA