title: Stable cell lines expressing baculovirus P35: resistance to apoptosis and nutrient stress, and increased glycoprotein secretion.
abstract: The baculovirus P35 protein is a caspase inhibitor that prevents the induction of apoptosis during infection of Sf21 cells by Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV). P35 inhibits the induction of apoptosis in a broad range of cells and circumstances. In this study, we examined the effects of constitutive cellular P35 expression on the response of cells to stressful culture conditions and on protein production in AcMNPV infected cells. Sf9 cell lines expressing AcMNPV P35 or an epitope-tagged P35 protein were generated using a double selection technique, involving selection in the antibiotic G418, followed by a second round of selection by exposure to actinomycin D, a potent inducer of apoptosis in Sf9 cells. Clonal cell lines were generated and examined for (1) resistance to actinomycin D induced apoptosis, (2) resistance to nutrient deprivation, and (3) baculovirus expression of intracellular and secreted proteins. When compared with Sf9 cells, two P35-expressing cell lines (Sf9P35AcV5-1 and Sf9P35AcV5-3) showed increased resistance to actinomycin D-induced apoptosis and a profound resistance to nutrient deprivation. When these cell lines were infected with a recombinant baculovirus expressing a secreted glycoprotein (secreted alkaline phosphatase), expression of the glycoprotein from these cells exceeded that from the parental Sf9 cells and was comparable to expression levels obtained from Tn5B1-4 cells, the best available cell line for high-level expression. Increased levels of protein secretion in Sf9P35AcV5-1 and Sf9P35AcV5-3 cells appear to result from a prolonged infection cycle and accumulation of the secreted glycoprotein.
authors.affiliation: Boyce Thompson Institute at Cornell University, Ithaca, New York 14853-1801, USA.
authors.forename: G
authors.initials: G
authors.lastname: Lin
authors.forename: G
authors.initials: G
authors.lastname: Li
authors.forename: R R
authors.initials: RR
authors.lastname: Granados
authors.forename: G W
authors.initials: GW
authors.lastname: Blissard
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis; D001483:Base Sequence; D002455:Cell Division; D002460:Cell Line; D002470:Cell Survival; D003470:Culture Media; D003609:Dactinomycin / Q000494:pharmacology; D017930:Genes, Reporter; D006023:Glycoproteins / Q000378:metabolism*; D051034:Inhibitor of Apoptosis Proteins; D008969:Molecular Sequence Data; D017924:Nucleopolyhedroviruses; D011994:Recombinant Proteins / Q000096:biosynthesis; D018411:Spodoptera / Q000166:cytology*; D014764:Viral Proteins / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D003470:Culture Media; D006023:Glycoproteins; D051034:Inhibitor of Apoptosis Proteins; D011994:Recombinant Proteins; D014764:Viral Proteins; C086430:inhibitor of apoptosis, Nucleopolyhedrovirus; D003609:Dactinomycin