Novel vectors for co-expression of two proteins in E. coli.
Two new vectors, pAC28 and pEGST, for the co-expression of recombinant genes in E. coli were developed. This two-plasmid system allows for an efficient expression and purification of large amounts of protein-protein complexes formed in bacterial cells. We have utilized this new system to express and isolate a stable complex of two human proteins, hematopoietic cell tyrosine phosphatase (HePTP) and mitogen-activated proteins kinase Erk2. This approach is useful for biochemical and structural studies of protein-protein interactions.
Kholod
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N
La Jolla Cancer Research Center, La Jolla, CA, USA.
Mustelin
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T
D003001:Cloning, Molecular / Q000379:methods; D004926:Escherichia coli / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D005822:Genetic Vectors / Q000235:genetics*; D006801:Humans; D019950:Mitogen-Activated Protein Kinase 1 / Q000235:genetics; D010957:Plasmids / Q000235:genetics*; D017027:Protein Tyrosine Phosphatases / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification*
D011994:Recombinant Proteins; D019950:Mitogen-Activated Protein Kinase 1; D017027:Protein Tyrosine Phosphatases