title: Hydrolysis of plasmid DNA catalyzed by Co(III) complex of cyclen attached to polystyrene.
abstract: Reactivity of the Co(III) complex of cyclen (CoCyc) in the hydrolytic cleavage of supercoiled pUC18 DNA leading to the formation of the corresponding open circular form was enhanced by >200 times upon attachment of CoCyc to cross-linked polystyrenes. Thus, half-lives as short as 40 min were achieved by the resin-based CoCyc in cleavage of the supercoiled DNA at 4 degrees C.
authors.affiliation: Artzyme Biotech Corporation, 403-1, Silim-2-Dong, Seoul 151-012, South Korea.
authors.forename: C S
authors.initials: CS
authors.lastname: Jeung
authors.forename: C H
authors.initials: CH
authors.lastname: Kim
authors.forename: K
authors.initials: K
authors.lastname: Min
authors.forename: S W
authors.initials: SW
authors.lastname: Suh
authors.forename: J
authors.initials: J
authors.lastname: Suh
mesh_terms: D003035:Cobalt / Q000737:chemistry*; D000080027:Cyclams; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D006571:Heterocyclic Compounds / Q000737:chemistry*; D006868:Hydrolysis; D007700:Kinetics; D009942:Organometallic Compounds / Q000737:chemistry*; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism*; D011137:Polystyrenes / Q000737:chemistry*; D011145:Polyvinyls / Q000737:chemistry
chemical_list: D000080027:Cyclams; D006571:Heterocyclic Compounds; D009942:Organometallic Compounds; D011137:Polystyrenes; D011145:Polyvinyls; C442399:cyclencobalt(III); C442400:poly(chloromethylstyrene-co-divinylbenzene); D003035:Cobalt; D004247:DNA; C038072:cyclen