Determination of 4,4'-dinitrocarbanilide (DNC), the active component of the antifertility agent nicarbazin, in chicken, duck, and goose plasma.
4,4'-Dinitrocarbanilide (DNC) was extracted from chicken, duck, and goose plasma and isolated by reversed-phase high-performance liquid chromatography. DNC was detected by ultraviolet absorbance at 347 nm and quantified by comparison to a calibration standard. Recovery data were determined by analyzing DNC-fortified control plasma. The mean recovery of DNC in fortified chicken plasma samples was 99.7 +/- 1.9% for 0.18 and 9.1 ppm DNC, and in fortified duck and goose plasma samples was 99.5 +/- 4.9% and 101.4 +/- 4.5%, respectively, for 0.18, 9.1, and 18 ppm DNC.
Primus
T M
TM
Analytical Chemistry Project, U.S. Department of Agriculture/Animal and Plant Health Inspection Service/National Wildlife Research Center, 4101 LaPorte Avenue, Fort Collins, Denver, Colorado 80521, USA. thomas.m.primus@usda.gov
Kohler
D J
DJ
Goodall
M A
MA
Yoder
C
C
Griffin
D
D
Miller
L
L
Johnston
J J
JJ
D000818:Animals; D002138:Calibration; D002645:Chickens; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004372:Ducks; D005298:Fertility / Q000187:drug effects*; D005777:Geese; D009528:Nicarbazin / Q000097:blood* / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
D009528:Nicarbazin