title: Glomerular endothelial cells and podocytes jointly synthesize laminin-1 and -11 chains.
abstract: BACKGROUND
  The glomerular basement membrane (GBM) originates in development from fusion of subendothelial and subepithelial matrices. Subsequently, newly synthesized subepithelial matrix is added as glomerular capillary loops expand. During GBM assembly, the laminin-1 heterotrimer (alpha 1, beta 1, and gamma 1 chains), initially expressed in vascular clefts of comma- and S-shaped bodies, is eventually replaced by laminin-11 (alpha 5, beta 2, and gamma 1 chains), which persists into maturation. The cellular source(s) of these laminins is not known and prompted this study.
  
  
  METHODS
  To determine which cells synthesize the various laminin chains, postfixation immunoelectron microscopy of developing mouse kidney was performed using monoclonal and polyclonal antibodies that specifically recognized laminin alpha 1, beta 1, alpha 5, or beta 2 chains.
  
  
  RESULTS
  Intracellular labeling for laminin alpha 1, beta 1 (laminin-1), and alpha 5 and beta 2 (laminin-11) chains was observed in developing glomerular endothelial cells and podocytes of comma- and S-shaped nephric figures. Laminin-1 was also seen in unfused GBMs at this stage, whereas laminin-11 was only found intracellularly. In capillary loop stage GBMs, laminin alpha 1 chain was completely absent, whereas labeling for laminin alpha 5 was intense, indicating rapid substitution between alpha chains. In contrast, laminin beta 1 chain labeling remained strong both intracellularly and in GBMs of capillary loop stage glomeruli, and beta 2 was up-regulated as well. In maturing stage glomeruli, beta 1 labeling declined, and alpha 5 and beta 2 remained at high levels intracellularly in both endothelial cells and podocytes and in GBMs.
  
  
  CONCLUSIONS
  Our results show that both endothelial cells and podocytes synthesize laminin-1 and -11 chains throughout glomerular development. The sustained and comparatively high level of laminin synthesis by endothelial cells was unexpected, suggesting that the endothelium may be an important source of GBM proteins in glomerular disease.
authors.affiliation: Department of Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City, Kansas 66160-7400, USA.
authors.forename: P L
authors.initials: PL
authors.lastname: St  John
authors.forename: D R
authors.initials: DR
authors.lastname: Abrahamson
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D004847:Epithelial Cells / Q000378:metabolism*; D007150:Immunohistochemistry; D007678:Kidney Glomerulus / Q000254:growth & development / Q000378:metabolism* / Q000648:ultrastructure; D007797:Laminin / Q000032:analysis / Q000096:biosynthesis* / Q000737:chemistry; D051379:Mice; D016253:Microscopy, Immunoelectron
chemical_list: D007797:Laminin; C121346:laminin 1; C097043:laminin alpha5; C058783:laminin beta2