Virus inactivation and protein modifications by ethyleneimines.
Virus inactivation by ethyleneimines was first introduced more than 30 years ago. Selective targeting of nucleic acids was reported for oligomeric ethyleneimines. In this study, trimeric ethyleneimine (TEI) was used to inactivate minute virus of mice (MVM; Parvoviridae) and Semliki forest virus (SFV; Togaviridae). The pH-dependency of the inactivation kinetics observed with MVM was different compared to the kinetics reported for other viruses. The higher inactivation rate at higher pH favoured the idea of a mechanism involving protein modifications. Alteration of the isoelectric point and changes in mass could be observed after treatment of soluble proteins with TEI. The uptake of MVM by host cells was reduced or completely blocked by TEI treatment, as shown by monitoring viral internalisation of DNA into target cells. The observed loss of virus infectivity coincided with the inhibition of virus uptake. Thus, virus inactivation by TEI is most likely also a result of chemical modifications of viral surface proteins.
Käsermann
F
F
Department of Chemistry and Biochemistry, University of Bern, Freiestrasse 3, 3012 Bern, Switzerland. kaersemann@ibc.unibe.ch
Wyss
K
K
Kempf
C
C
D000330:Aedes; D000818:Animals; D001388:Aziridines / Q000494:pharmacology*; D002213:Capsid / Q000187:drug effects; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D003588:Cytopathogenic Effect, Viral; D004279:DNA, Viral / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D007526:Isoelectric Point; D007700:Kinetics; D051379:Mice; D008915:Minute Virus of Mice / Q000187:drug effects* / Q000254:growth & development; D009211:Myoglobin / Q000378:metabolism; D010047:Ovalbumin / Q000378:metabolism; D011499:Protein Processing, Post-Translational / Q000187:drug effects; D012672:Semliki forest virus / Q000187:drug effects* / Q000254:growth & development; D021241:Spectrometry, Mass, Electrospray Ionization; D013997:Time Factors; D014709:Vero Cells; D014759:Viral Envelope Proteins / Q000187:drug effects; D017735:Virus Latency / Q000187:drug effects
D001388:Aziridines; D004279:DNA, Viral; D009211:Myoglobin; D014759:Viral Envelope Proteins; C033132:aziridine; D010047:Ovalbumin