Immunohistochemical analyses of cell cycle-related proteins, apoptosis, and proliferation in pituitary adenomas.
To analyze the cell cycle regulatory mechanisms in the growth of pituitary adenomas, we investigated immunohistochemically the expression of the cell cycle-related proteins cyclin A and p27 in 48 pituitary adenomas. The frequency of apoptosis and the proliferative potential were also examined. The percentage of apoptotic cells was evaluated by immunohistochemical analysis using the anti-single-strand DNA antibody. The proliferative potential was assessed using the anti-Ki-67 antibody. The mean cyclin A labeling index (LI) for the non-recurrent group was 1.03% and for the recurrent group 2.31%. A positive linear correlation between cyclin A LI and Ki-67 LI was found. The mean p27 LI for the non-recurrent group was 67.4% and for the recurrent group 47.0%. There were significant differences in cyclin A LI and p27 LI between the non-recurrent group and the recurrent group. The mean apoptotic rate for the non-recurrent group was 0.87% and for the recurrent group 1.05%. There was no significant difference. Multivariate regression analysis revealed that high cyclin A LI and high Ki-67 LI were significant factors for shorter progression-free survival. The results suggest that the cyclin A LI is a useful prognostic factor in pituitary adenomas. (J Histochem Cytochem 49:1193-1194, 2001)
Nakabayashi
H
H
Department of Neurosurgery, Saiseikai Ibaraki Hospital, 1-1-24 Mitsukeyama, Ibaraki, Osaka K 573-0701, Japan. nakabayashi@kcat.zaq.ne.jp
Sunada
I
I
Hara
M
M
D000236:Adenoma / Q000378:metabolism* / Q000473:pathology*; D000328:Adult; D017209:Apoptosis; D018797:Cell Cycle Proteins / Q000378:metabolism*; D002455:Cell Division; D019925:Cyclin A / Q000378:metabolism; D050760:Cyclin-Dependent Kinase Inhibitor p27; D018844:Cyclin-Dependent Kinases / Q000037:antagonists & inhibitors; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D010911:Pituitary Neoplasms / Q000378:metabolism* / Q000473:pathology*; D025521:Tumor Suppressor Proteins
D018797:Cell Cycle Proteins; D019925:Cyclin A; D025521:Tumor Suppressor Proteins; D050760:Cyclin-Dependent Kinase Inhibitor p27; D018844:Cyclin-Dependent Kinases