title: On-line immunoaffinity extraction followed by high-performance liquid chromatography and radioimmunoassay for a novel retinobenzoic acid, AM-80, in human plasma.
abstract: On-line coupled immunoaffinity chromatography and reversed-phase high performance liquid chromatography (on-line IAC-HPLC) with detection by radioimmunoassay (RIA) was developed for 4-[(5,6,7,8-tetrahydro-5,5,8,8-tetramethyl-2-naphthyl)-carbamoyl] benzoic acid (Am-80) in human plasma. A 0.05-mL sample was directly loaded onto an immunoaffinity pre-column packed with immobilized polyclonal antibodies against Am-80. The immunoaffinity extract was then automatically introduced to reversed-phase
authors.affiliation: Shionogi Research Laboratories, Shionogi & Co, Ltd, Toyonaka, Osaka, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Itoh
authors.forename: G
authors.initials: G
authors.lastname: Kominami
mesh_terms: D001565:Benzoates / Q000097:blood*; D002846:Chromatography, Affinity / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D011863:Radioimmunoassay / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013764:Tetrahydronaphthalenes / Q000097:blood*
chemical_list: D001565:Benzoates; D013764:Tetrahydronaphthalenes; C061133:tamibarotene