Presence of neuronal nitric oxide synthase in autonomic and sensory ganglion neurons innervating the lacrimal glands of the cat: an immunofluorescent and retrograde tracer double-labeling study.
It is generally considered that parasympathetic postganglionic nerve fibers innervating the lacrimal gland (LG) arise from the pterygopalatine ganglion (PPG), while sympathetic and sensory innervations arise from the superior cervical ganglion (SCG) and trigeminal ganglion (TG), respectively. Recently, we reported for the first time that the parasympathetic innervation of the cat LG was also provided by the otic ganglion (OG) and ciliary ganglion (CG), and that the sensory innervation was also provided by the superior vagal ganglion (SVG) and superior glossopharyngeal ganglion (SGG). To determine if nitric oxide (NO) is a neurotransmitter of the autonomic and sensory neurons innervating the LG, we injected the cholera toxin B subunit (CTB) as a retrograde tracer into the cat LG, and used double-labeling fluorescent immunohistochemistry for CTB and nitric oxide synthase (NOS). We found that NOS-/CTB-immunofluorescent double-labeled perikarya were localized in the PPG, OG, TG, SVG and SGG, but not in the CG and SCG. The highest numbers of NOS-/CTB-immunofluorescent double-labeled neurons were found in the PPG and TG. In addition, we examined the presence of nitrergic nerve fibers in the LG using NADPH-d histochemistry and found that a large amount of NADPH-d-stained nerve fibers were distributed around the glandular acini and in the walls of glandular ducts and blood vessels. This study provides the first direct evidence showing that NO may act as a neurotransmitter or modulator involved in the parasympathetic and sensory regulation of lacrimal secretion and blood circulation, but may not be implicated in the sympathetic control of LG activities, and that nitrergic nerve fibers in the LG arise mainly from parasympathetic postganglionic neurons in the PPG and sensory neurons in the TG. The present results suggest that NO plays an important role in the regulation of LG activities.
Cheng
S B
SB
Department of Anatomy, Faculty of Medicine, Kagoshima University, Sakuragaoka 8-35-1, Kagoshima 890-8520, Japan.
Kuchiiwa
S
S
Kuchiiwa
T
T
Nonaka
S
S
Nakagawa
S
S
D000818:Animals; D002415:Cats; D002452:Cell Count; D002772:Cholera Toxin / Q000493:pharmacokinetics; D005260:Female; D005455:Fluorescent Antibody Technique; D005726:Ganglia, Parasympathetic / Q000166:cytology / Q000201:enzymology*; D017950:Ganglia, Sensory / Q000166:cytology / Q000201:enzymology*; D005728:Ganglia, Sympathetic / Q000166:cytology / Q000201:enzymology; D005930:Glossopharyngeal Nerve / Q000166:cytology / Q000201:enzymology; D007765:Lacrimal Apparatus / Q000166:cytology / Q000201:enzymology* / Q000294:innervation*; D008297:Male; D009252:NADPH Dehydrogenase / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000201:enzymology*; D009569:Nitric Oxide / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000378:metabolism*; D017777:Parasympathetic Fibers, Postganglionic / Q000166:cytology / Q000201:enzymology*; D017779:Sympathetic Fibers, Postganglionic / Q000166:cytology / Q000201:enzymology; D012668:Trigeminal Ganglion / Q000166:cytology / Q000201:enzymology; D014630:Vagus Nerve / Q000166:cytology / Q000201:enzymology
D009569:Nitric Oxide; D002772:Cholera Toxin; D019001:Nitric Oxide Synthase; D009252:NADPH Dehydrogenase