title: Isolation of Helicobacter pylori after transport from a regional laboratory of gastric biopsy specimens in saline, Portagerm pylori or cultured on chocolate agar.
abstract: Multiple gastric biopsies were taken from 288 patients in Port Lincoln, South Australia. One biopsy was used for a CLOtest and the other three were transported to a central laboratory in Adelaide in physiological saline, Portagerm pylori transport medium or after culture on a chocolate agar plate which was placed in a Biobag. Helicobacter pylori was isolated from 18.3% of patients. There was a 95.7% concordance between culture results and the CLOtest result. Recovery rates after transport on chocolate agar, Portagerm pylori and in saline were 90.2, 90.2 and 84.3%, respectively.
authors.affiliation: Department of Clinical Microbiology and Infectious Diseases, Institute of Medical and Veterinary Science at The Queen Elizabeth Hospital, Woodville, South Australia, Australia. david.grove@imvs.sa.gov.au
authors.forename: D I
authors.initials: DI
authors.lastname: Grove
authors.forename: R A
authors.initials: RA
authors.lastname: McLeay
authors.forename: K E
authors.initials: KE
authors.lastname: Byron
authors.forename: G
authors.initials: G
authors.lastname: Koutsouridis
mesh_terms: D000362:Agar; D001706:Biopsy; D003470:Culture Media; D016099:Endoscopy, Gastrointestinal; D016481:Helicobacter Infections / Q000382:microbiology* / Q000473:pathology; D016480:Helicobacter pylori / Q000254:growth & development / Q000302:isolation & purification*; D006767:Hospitals, District; D006801:Humans; D011706:Pyloric Antrum / Q000382:microbiology* / Q000473:pathology; D012965:Sodium Chloride; D013048:Specimen Handling / Q000379:methods*; D014186:Transportation
chemical_list: D003470:Culture Media; D012965:Sodium Chloride; D000362:Agar