title: Survival of boar spermatozoa frozen in diluents of varying osmolality.
abstract: We investigated the effects of freezing diluents of differing levels of osmolality on boar sperm cryosurvival. The spermatozoa were frozen using a pellet technique. Cryosurvival was evaluated in terms of motility, intact acrosomes and membrane integrity. The motility parameters were assessed using a computer-assisted sperm motility analysis (CASA) system. Acrosomal status was monitored by means of FITC-labeled peanut agglutinin, and membrane integrity was evaluated after double staining with SYBR-14 and propidium iodide. At 3 h of incubation after thawing, the highest motility was found in the 420 mOsm/kg group, and progressive motiLity in the 420 to 580 mOsm/kg groups was higher than that in the hypo- (225 mOsm/kg) and iso-osmotic (290 mOsm/kg) groups (P < 0.05). The intact acrosomes of the spermatozoa frozen in the 510 and 580 mOsm/kg BF5 diluents were more numerous than in other groups (P < 0.05). The 420 and 510 mOsm/kg groups yielded maximal values of post-thaw membrane integrity. These observations obtained in the present study indicate that moderately hypertonic BF5 diluents are favorable for the cryopreservation of boar spermatozoa.
authors.affiliation: Animal Reproduction, Faculty of Applied Biological Science, Hiroshima University, Higashi-Hiroshima, Japan.
authors.forename: W X
authors.initials: WX
authors.lastname: Zeng
authors.forename: M
authors.initials: M
authors.lastname: Shimada
authors.forename: N
authors.initials: N
authors.lastname: Isobe
authors.forename: T
authors.initials: T
authors.lastname: Terada
mesh_terms: D020101:Acrosome Reaction / Q000502:physiology; D000818:Animals; D002462:Cell Membrane / Q000502:physiology; D004396:Coloring Agents / Q000737:chemistry; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary*; D005456:Fluorescent Dyes / Q000737:chemistry; D008297:Male; D008856:Microscopy, Fluorescence / Q000662:veterinary; D009930:Organic Chemicals; D009994:Osmolar Concentration; D011419:Propidium / Q000737:chemistry; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D013081:Sperm Motility / Q000502:physiology; D013094:Spermatozoa / Q000502:physiology*; D013552:Swine / Q000502:physiology; D013556:Swine, Miniature / Q000502:physiology*
chemical_list: D004396:Coloring Agents; D005456:Fluorescent Dyes; D009930:Organic Chemicals; C093207:SYBR-14; D011419:Propidium