Polymerase chain reaction and denaturing gradient gel electrophoresis monitoring of fecal bifidobacterium populations in a prebiotic and probiotic feeding trial.
A culture-independent approach based on genus-specific PCR and denaturing gradient gel electrophoresis (DGGE) was used to monitor qualitative changes in fecal bifidobacterial communities in a human feeding trial. DNA was extracted directly from feces and bifidobacterial 16S rDNA sequences were amplified using genus-specific PCR. The PCR fragments were subsequently separated in a sequence-specific manner by DGGE in order to obtain a profile of bifidobacterial fragments. The DGGE profiles revealed that in general, administration for two weeks of galactooligosaccharide and/or Bifidobacterium lactis Bb-12 (8 g and 3 x 10(10) cfu per day, respectively) did not affect the qualitative composition of the indigenous Bifidobacterium population, while B. lactis Bb-12 transiently colonised the gut.
Satokari
R M
RM
Laboratory of Microbiology, Wageningen University, The Netherlands. reetta.satokari@vtt.fi
Vaughan
E E
EE
Akkermans
A D
AD
Saarela
M
M
De Vos
W M
WM
D000328:Adult; D001644:Bifidobacterium / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000032:analysis; D004435:Eating; D004587:Electrophoresis, Agar Gel; D005243:Feces / Q000382:microbiology*; D005260:Female; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000008:administration & dosage; D016133:Polymerase Chain Reaction / Q000379:methods*; D019936:Probiotics / Q000008:administration & dosage*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial; D009844:Oligosaccharides; D012336:RNA, Ribosomal, 16S