Production of aromatic minimal polyketides by the daunorubicin polyketide synthase genes reveals the incompatibility of the heterologous DpsY and JadI cyclases.
Our investigations into whether the biosynthesis of a linearly fused ring system of an aromatic polyketide (jadomycin) could be modified to produce an angularly fused system (daunorubicin) and vice versa showed that introduction of the respective cyclases did not have the desired effect. Genes from the daunorubicin pathway produced a novel 21-carbon polyketide.
Wohlert
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School of Pharmacy, University of Wisconsin, 425 N. Charter Street, Madison, Wisconsin 53706, USA.
Wendt-Pienkowski
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Bao
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Hutchinson
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D000105:Acetyl Coenzyme A / Q000378:metabolism; D000429:Alcohol Oxidoreductases / Q000378:metabolism; D000880:Anthraquinones / Q000737:chemistry; D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000737:chemistry*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D003500:Cyclization; D003630:Daunorubicin / Q000737:chemistry; D004317:Doxorubicin / Q000737:chemistry; D015870:Gene Expression; D005798:Genes, Bacterial; D019751:Intramolecular Transferases / Q000378:metabolism; D007546:Isoquinolines / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D015394:Molecular Structure; D009097:Multienzyme Complexes / Q000235:genetics* / Q000378:metabolism*; D010957:Plasmids; D011753:Pyrones / Q000737:chemistry / Q000378:metabolism*; D013302:Streptomyces / Q000201:enzymology* / Q000235:genetics* / Q000502:physiology; D000095602:Angucyclines and Angucyclinones
D000105:Acetyl Coenzyme A; D000429:Alcohol Oxidoreductases; D000880:Anthraquinones; D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D003630:Daunorubicin; D004317:Doxorubicin; D019751:Intramolecular Transferases; D007546:Isoquinolines; D009097:Multienzyme Complexes; D011753:Pyrones; C112173:DpsY protein, Streptomyces peucetius; C436529:SEK43; C091616:daunorubicin-doxorubicin polyketide synthase; C081750:jadomycin B; C003289:rabelomycin; D000095602:Angucyclines and Angucyclinones; C042374:aklanonic acid; C087192:polyketide synthase ketoreductase; C436528:jadI protein, Streptomyces