title: Both B and gammadelta TCR(+) lymphocytes regulate alphabeta TCR(+) lymphocytes involved in superantigen specific responses.
abstract: Endogenous superantigens (SAg) presented by MHC class II IA molecules induce slow-evolving negative selection of alpha beta T cells. The role of both B and gamma delta T cells on the regulation of these SAg-specific alpha beta T cell responses was addressed in IA(b+)IE(b-) C57BL/6 mice bearing genetically induced B cell and gamma delta T cell deficiencies. B lymphocytes were required in the negative selection of Vbeta5(+)/Vbeta12(+) CD4(+) T cells. In contrast, gamma delta T cells positively stimulated the utilization of the same SAg-responsive alpha beta T cell subsets. These differences started in mature CD4(+) thymocytes and extended to naive T cell pools for B cell negative selection, and up to memory T cells for gamma deltaT cell influences. The levels of SAg-responsive T cells did not vary between C57BL/6 and double deficient (B cell and gamma delta T cell-deficient) congenic mice, implying that both B and gamma delta T cells acted through independent mechanisms.
authors.affiliation: Centro de Biología Molecular Severo Ochoa, CSIC-UAM, Campus de Cantoblanco, Madrid, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Izcue
authors.forename: G
authors.initials: G
authors.lastname: Morales
authors.forename: S
authors.initials: S
authors.lastname: Minguet
authors.forename: A
authors.initials: A
authors.lastname: Sánchez-Movilla
authors.forename: P
authors.initials: P
authors.lastname: Morales
authors.forename: J A
authors.initials: JA
authors.lastname: Martínez-M
authors.forename: M L
authors.initials: ML
authors.lastname: Gaspar
authors.forename: M A
authors.initials: MA
authors.lastname: Marcos
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D007075:Immunoglobulin M / Q000235:genetics; D007135:Immunoglobulin Variable Region; D007156:Immunologic Memory; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000032:analysis*; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000032:analysis* / Q000235:genetics; D018089:Superantigens / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology; D013950:Thymus Gland / Q000276:immunology
chemical_list: D007075:Immunoglobulin M; D007135:Immunoglobulin Variable Region; D016693:Receptors, Antigen, T-Cell, alpha-beta; D016692:Receptors, Antigen, T-Cell, gamma-delta; D018089:Superantigens