title: Elongated conformers of charge states +11 to +15 of bovine ubiquitin studied using ESI-FAIMS-MS.
abstract: Recent advancements in high-field asymmetric waveform ion mobility spectrometry (FAIMS) have led to significant improvements in the application of this technology to the study of protein conformers. Compared with previous work, the maximum value of the separation voltage (i.e., the dispersion voltage) has increased, thereby enabling multiple, elongated conformers of individual charge states of bovine ubiquitin to be separated in the gas phase (e.g., four conformers of each of the +11 and +12 charge states were separated). The use of a carrier gas mixture of 40% nitrogen and 60% helium changed the separation selectivity compared with pure nitrogen and enhanced the signal intensity, especially for the +14 and +15 charge states (the latter was not detected in a nitrogen carrier gas). Conformer cross sections were determined using the FAIMS/energy-loss method and found to be similar within a given charge state. The cross sections for conformers of charge states +13, + 14, and +15 plateau at about 2000 A2 suggesting that the structure of bovine ubiquitin is essentially unfolded after the addition of the 13th proton.
authors.affiliation: National Research Council of Canada, Institute for National Measurement Standards, Ottawa, Ontario.
authors.forename: R W
authors.initials: RW
authors.lastname: Purves
authors.forename: D A
authors.initials: DA
authors.lastname: Barnett
authors.forename: B
authors.initials: B
authors.lastname: Ells
authors.forename: R
authors.initials: R
authors.lastname: Guevremont
mesh_terms: D000465:Algorithms; D000818:Animals; D002417:Cattle; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D011487:Protein Conformation; D021241:Spectrometry, Mass, Electrospray Ionization; D014452:Ubiquitins / Q000737:chemistry*
chemical_list: D014452:Ubiquitins