Bacterial cellulose production under oxygen-enriched air at different fructose concentrations in a 50-liter, internal-loop airlift reactor.
Bacterial cellulose (BC) production by Acetobacter xylinum subsp. sucrofermentans BPR2001 was carried out in a 50-1 internal-loop airlift reactor in air at an initial fructose concentration of 40 g/l. The BC production rate was 0.059 g/l per h. When oxygen-enriched air was supplied instead of air, the BC production rate increased to 0.093 g/l per h, and the BC yield was enhanced from 11% in air to 18%. When the initial fructose concentrations were varied from 30 to 70 g/l, the highest BC yield (35%) the highest production rate (0.22 g/l x per h), and the highest concentration of BC produced (10.4 g/l) were observed at 60-70 g/l fructose. From the carbon mass balance calculated at the final stage of cultivation, it was observed that enhanced BC production was reflected as a decrease in both CO2 evolution and the concentration of other unknown substances, suggesting the efficient utilization of energy for BC synthesis despite O2 limitation.
Chao
Y
Y
Research Laboratory of Resources Utilization, Tokyo Institute of Technology, Yokohama, Japan.
Sugano
Y
Y
Shoda
M
M
D000091:Acetobacter / Q000254:growth & development / Q000378:metabolism*; D000388:Air; D019149:Bioreactors; D002245:Carbon Dioxide / Q000378:metabolism; D002482:Cellulose / Q000096:biosynthesis; D003470:Culture Media; D005632:Fructose / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism
D003470:Culture Media; D002245:Carbon Dioxide; D005632:Fructose; D002482:Cellulose; D010100:Oxygen