Phosphorylation by PKA of a site unique to B-Raf kinase.
The Raf kinases serve as central intermediates to relay signals from Ras to ERK. Cell-specific effects of these signals on growth, differentiation and survival can be observed due to the recruitment of different isoenzymes of the Raf family. The in vitro phosphorylation of a site unique to B-Raf (Ser429) has been proposed to be responsible for the negative regulation of the isoenzyme by Akt. Using phosphopetide mapping and site-directed mutagenesis we showed that Ser429 is phosphorylated upon cAMP elevation in PC12 cells and proposed that PKA is a major kinase phosphorylating the B-Raf-specific site in vivo.
König
S
S
UPR 2197 CNRS, institut de neurobiologie Alfred-Fessard, 1, avenue de la terrasse, Bâtiment 33, 91198 Gif-sur-Yvette, France. koenig@iaf.cnrs-gif.fr
Guibert
B
B
Morice
C
C
Vernier
P
P
Barnier
J V
JV
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D019556:COS Cells; D002851:Chromatography, High Pressure Liquid; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005982:Glutathione Transferase / Q000235:genetics; D007527:Isoenzymes / Q000737:chemistry*; D007546:Isoquinolines / Q000494:pharmacology; D016297:Mutagenesis, Site-Directed; D016716:PC12 Cells; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D010449:Peptide Mapping; D010766:Phosphorylation; D010768:Phosphoserine / Q000378:metabolism; D019908:Proto-Oncogene Proteins c-raf / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D013449:Sulfonamides; D014357:Trypsin
D004791:Enzyme Inhibitors; D007527:Isoenzymes; D007546:Isoquinolines; D010446:Peptide Fragments; D011993:Recombinant Fusion Proteins; D013449:Sulfonamides; D010768:Phosphoserine; D005576:Colforsin; D000242:Cyclic AMP; D005982:Glutathione Transferase; D019908:Proto-Oncogene Proteins c-raf; D017868:Cyclic AMP-Dependent Protein Kinases; D014357:Trypsin; C063509:N-(2-(4-bromocinnamylamino)ethyl)-5-isoquinolinesulfonamide