[Expression of tissue-specific genes with progression of mouse hepatocellular carcinoma].
Expression of hepatocyte-specific genes in slow- and fast-growing hepatocellular mouse carcinomas was studied. The fast-growing poorly differentiated passaged hepatocarcinoma (fHC) originated from the well-differentiated slow-growing variant (sHC). In contrast to the parental hepatocarcinoma, in fHC the expression of the hepatocyte nuclear factor 4 (HNF4), in fHC a key factor responsible for hepatocyte differentiation, and several HNF-4-responsive genes, such as those for transferrin, transthyretin, hepatocyte nuclear factor 1 (HNF1), and serum albumin, was significantly suppressed. The expression of exogenous HNF4 in the fHC cell culture partially restored the expression of hepatocyte marker genes and the appearance of epithelial cell islands in the culture. The described system may serve as a convenient model for further analysis of mechanisms underlying hepatocarcinogenesis and liver tumor progression.
Varga
E V
EV
Institute of Carcinogenesis, Blokhin Cancer Research Center, Russian Academy of Medical Science, Moscow, 115478 Russia.
Cheremnova
O A
OA
Ovchinnikov
D A
DA
Shapiguzov
A Iu
AIu
Kudriavtseva
E I
EI
Morozova
O V
OV
Engel'gardt
N V
NV
Lazarevich
N L
NL
D000818:Animals; D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; D002454:Cell Differentiation; D002455:Cell Division; D004268:DNA-Binding Proteins; D015972:Gene Expression Regulation, Neoplastic; D051557:Hepatocyte Nuclear Factor 4; D008114:Liver Neoplasms, Experimental / Q000235:genetics* / Q000473:pathology; D051379:Mice; D009928:Organ Specificity; D010750:Phosphoproteins / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*
D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; D004268:DNA-Binding Proteins; D051557:Hepatocyte Nuclear Factor 4; D010750:Phosphoproteins; C501970:Tcfl4 protein, mouse; D014157:Transcription Factors
Ekspressiia tkanespetsificheskikh genov pri progressii gepatokartsinom myshi.