title: Purification of saponin compounds in Bupleurum falcatum by solvent partitioning and preparative LC.
abstract: Saponin compounds (saikosaponin c, a, and d) in Bupleurum falcatum were partially purified by solvent partitioning of the herbal extract using diethyl ether, distilled water, n-butanol, and acetone. After separation of the saponins by preparative LC, the purity of each saikosaponin was more than 94%. The identities of purified individual saikosaponins were confirmed by TLC, analytical LC, and fast-atom bombardment mass spectrometry.
authors.affiliation: Korea Food Research Institute, Songnam-si, Kyonggi-do, Republic of Korea. k9130sen@hanmail.net
authors.forename: N
authors.initials: N
authors.lastname: Kim
authors.forename: I S
authors.initials: IS
authors.lastname: Park
mesh_terms: D029972:Bupleurum / Q000737:chemistry*; D002853:Chromatography, Liquid; D015394:Molecular Structure; D009828:Oleanolic Acid / Q000031:analogs & derivatives*; D012502:Sapogenins / Q000737:chemistry / Q000302:isolation & purification*; D012503:Saponins / Q000302:isolation & purification*; D012997:Solvents
chemical_list: D012502:Sapogenins; D012503:Saponins; D012997:Solvents; D009828:Oleanolic Acid; C025759:saikosaponin D