Rainbow trout (Onchorhynchus mykiss) hepatic glucose transporter.
We report identification of a rainbow trout hepatic glucose transporter sharing 58% and 52% amino acid identity with avian and mammalian GLUT2 sequences, respectively. The functionality of OnmyGLUT2 was assessed by expression in rainbow trout embryos. We also measured the transport of hexose in isolated rainbow trout hepatocytes. Inhibition of 3-O-methylglucose uptake by cytochalasin B, phloretin and 2-deoxy-D-glucose suggested the existence of a functional facilitative transporter in these cells. Expression of OnmyGLUT2 was found in the liver, kidney and intestine.
Krasnov
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Institute of Applied Biotechnology, University of Kuopio, P.O. Box 1627, FIN-70211, Kuopio, Finland. kranov@uku.fi
Teerijoki
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Mölsä
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D019325:3-O-Methylglucose / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000096:biosynthesis / Q000737:chemistry; D051273:Glucose Transporter Type 2; D008099:Liver / Q000201:enzymology / Q000378:metabolism; D008969:Molecular Sequence Data; D009004:Monosaccharide Transport Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D017686:Oncorhynchus mykiss / Q000196:embryology / Q000235:genetics*; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment
D018076:DNA, Complementary; D051273:Glucose Transporter Type 2; D009004:Monosaccharide Transport Proteins; D019325:3-O-Methylglucose