title: Determination of mevalonic acid in human urine as mevalonic acid lactone by gas chromatography-mass spectrometry.
abstract: Mevalonic acid in urine is converted to its lactone form by incubation overnight at acidic pH, extracted and analysed by GC-MS. The lower limit of quantitation of 7.5 ng/ml provides adequate sensitivity to measure changes in urinary excretion of MVA following administration of drugs which affect cholesterol synthesis. The assay is linear up to 300 ng/ml and has acceptable precision (<15%) and accuracy (<+/-20%) across the calibration range.
authors.affiliation: Syngenta CTL, Alderley Park, Nr. Macclesfield, Cheshire, UK. Bruce.woollen@syngenta.com
authors.forename: B H
authors.initials: BH
authors.lastname: Woollen
authors.forename: P C
authors.initials: PC
authors.lastname: Holme
authors.forename: W J
authors.initials: WJ
authors.lastname: Northway
authors.forename: P D
authors.initials: PD
authors.lastname: Martin
mesh_terms: D002138:Calibration; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D007783:Lactones / Q000652:urine*; D008798:Mevalonic Acid / Q000652:urine*; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D007783:Lactones; D008798:Mevalonic Acid