Cytogenetic analysis and construction of a BAC contig across a common neocentromeric region from 9p.
Over 40 cases of neocentric marker chromosomes, without detectable alpha-satellite DNA, have been reported. Although these have originated from many different chromosomes, a few of these chromosomes have been involved in multiple cases of marker formation. In this study, two different markers originating from the short arm of chromosome 9 were analyzed, identifying a common neocentromeric region. A bacterial artificial chromosome (BAC) contig extending over more than 900 kb has been assembled across this neocentromeric region. Fluorescent in situ hybridization and immunofluorescence assays (CENP-C and CENP-E) have localized the neocentromere to a 500 kb region. Preliminary analysis of DNA sequences in this neocentromere revealed a highly AT-rich region, which also has an increase in the level of retroviral elements compared with the average levels in the genome.
Satinover
D L
DL
Department of Genetics and Center for Human Genetics, Case Western Reserve University School of Medicine and University Hospitals Cleveland, OH 44106-9959, USA.
Vance
G H
GH
Van Dyke
D L
DL
Schwartz
S
S
D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D002503:Centromere; D002869:Chromosome Aberrations; D022202:Chromosomes, Artificial, Bacterial; D002899:Chromosomes, Human, Pair 9; D006224:Cricetinae; D017931:DNA Primers; D005455:Fluorescent Antibody Technique; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping
D017931:DNA Primers