Physical and functional interaction between viral and cellular proteins modulate JCV gene transcription.
The lytic phase of JC virus (JCV) appears to be highly complex and remains elusive. A growing body of experimental evidence suggests that the regulation of JCV gene expression and replication requires, in addition to the presence of specific transcription factors, cooperativity between viral and cellular regulatory proteins. This cooperativity may be accomplished by physical interaction of the participant proteins on and/or off the viral DNA sequence. Here, we present evidence of specific physical and functional interaction between a cellular factor, YB-1, and the JCV early protein, T-antigen, and showed that both proteins play important roles in JCV gene transcription. Additionally, our data indicate that YB-1 also functionally interact with another viral protein, designated agnoprotein, which is expressed late during the course of infection, adding further complexity to the currently known picture on JCV gene regulation.
Safak
M
M
Center for Neurovirology and Cancer Biology, College of Science and Technology, Temple University, Philadelphia, Pennsylvania 19122, USA.
Khalili
K
K
D000957:Antigens, Viral, Tumor / Q000235:genetics; D022762:CCAAT-Enhancer-Binding Proteins / Q000235:genetics; D004268:DNA-Binding Proteins; D015967:Gene Expression Regulation, Viral / Q000502:physiology*; D007577:JC Virus / Q000235:genetics*; D051839:NFI Transcription Factors; D009687:Nuclear Proteins; D014157:Transcription Factors; D015533:Transcriptional Activation / Q000502:physiology*; D014764:Viral Proteins / Q000235:genetics*; D054334:Viral Regulatory and Accessory Proteins; D051840:Y-Box-Binding Protein 1; D000086082:Viroporin Proteins
D000957:Antigens, Viral, Tumor; D022762:CCAAT-Enhancer-Binding Proteins; D004268:DNA-Binding Proteins; D051839:NFI Transcription Factors; D009687:Nuclear Proteins; D014157:Transcription Factors; D014764:Viral Proteins; D054334:Viral Regulatory and Accessory Proteins; D051840:Y-Box-Binding Protein 1; C070856:YBX1 protein, human; C042559:agnoprotein, polyomavirus; D000086082:Viroporin Proteins