Selective labeling of pulmonary surfactant protein SP-C in organic solution.
Pulmonary surfactant protein SP-C has been isolated from porcine lungs and treated with dansyl isothiocyanate in chloroform:methanol 2:1 (v/v) solutions,under conditions optimized to introduce a single dansyl group covalently attached to the N-terminalamine group of the protein without loss of its native thioesther-linked palmitic chains. The resulting derivative Dans-SP-C conserves the secondary structure of native SP-C as well as the ability to promote interfacial adsorption of DPPC suspensions and to affect the thermotropic behavior of DPPC bilayers. This derivative can be used to characterize lipid-protein and protein-protein interactions of a native-like SP-C in lipid/protein complexes.
Plasencia
I
I
Departamento de Bioquímica, Facultad de Biología, Madrid, 28040, Spain.
Cruz
A
A
López-Lacomba
J L
JL
Casals
C
C
Pérez-Gil
J
J
D000818:Animals; D002942:Circular Dichroism; D003619:Dansyl Compounds / Q000737:chemistry; D005456:Fluorescent Dyes / Q000032:analysis; D008055:Lipids / Q000737:chemistry*; D013058:Mass Spectrometry; D019308:Palmitic Acid / Q000737:chemistry; D017433:Protein Structure, Secondary; D011510:Proteolipids / Q000737:chemistry / Q000302:isolation & purification*; D011663:Pulmonary Surfactants / Q000737:chemistry / Q000302:isolation & purification*; D012996:Solutions; D013050:Spectrometry, Fluorescence; D013552:Swine
D003619:Dansyl Compounds; D005456:Fluorescent Dyes; D008055:Lipids; D011510:Proteolipids; D011663:Pulmonary Surfactants; D012996:Solutions; D019308:Palmitic Acid; C029038:dansyl chloride