title: A variant form of myelodysplastic syndrome with Ph- minor-BCR/ABL transcript.
abstract: This study concerns a patient with minor (m)-BCR/ABL transcript-positive and Philadelphia (Ph) chromosome-negative myelodysplastic syndrome (MDS). The patient was a 78-year-old man whose condition was diagnosed as refractory anemia with excess of blasts in transformation. Molecular genetic studies, using reverse transcriptase polymerase chain reaction analysis detected m-BCR/ABL messenger RNA. We used spectral karyotyping to analyze metaphase cells but could not detect a Ph chromosome. Fluorescence in situ hybridization, however, revealed fusion signals of BCR and ABL probes on an apparently normal chromosome 22.
authors.affiliation: Department of Hematology and Oncology, Shimane Prefectural Central Hospital, Izumo, Japan. wakayama@spch.izumo.shimane.jp
authors.forename: T
authors.initials: T
authors.lastname: Wakayama
authors.forename: Y
authors.initials: Y
authors.lastname: Maniwa
authors.forename: H
authors.initials: H
authors.lastname: Ago
authors.forename: N
authors.initials: N
authors.lastname: Kakazu
authors.forename: T
authors.initials: T
authors.lastname: Abe
mesh_terms: D000368:Aged; D000754:Anemia, Refractory, with Excess of Blasts / Q000097:blood / Q000145:classification* / Q000175:diagnosis / Q000235:genetics / Q000473:pathology; D001853:Bone Marrow / Q000473:pathology; D002886:Chromosomes, Human, Pair 17 / Q000235:genetics / Q000648:ultrastructure; D002892:Chromosomes, Human, Pair 22 / Q000235:genetics; D002899:Chromosomes, Human, Pair 9 / Q000235:genetics; D017809:Fatal Outcome; D016044:Fusion Proteins, bcr-abl / Q000235:genetics*; D006801:Humans; D016130:Immunophenotyping; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D008297:Male; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014178:Translocation, Genetic
chemical_list: D012333:RNA, Messenger; D016044:Fusion Proteins, bcr-abl