Identification of a 2,6-dichloroisonicotinic-acid-sensitive protein kinase from tobacco by affinity chromatography on benzothiadiazole-sepharose and NIM-metal chelate adsorbent.
In the search for the target site of inducers of systemic acquired resistance (SAR), a BTH-binding protein kinase (BBPK) has been identified from tobacco by affinity chromatography on benzothiadiazole-sepharose (CGA 324041-sepharose) and NIM-metal chelate affinity resin. The substrate selectivity of the isolated enzyme (phosphorylation of histone type III-S, I kappa B alpha,IkB alpha S32A/S36A and NIM1) suggested a possible BBPK-mediated regulation of NIM1 in tobacco. The measurement of the effect of different SAR-inducers showed an inhibition of BBPK by 2,6-dichloroisonicotinic acid (INA) and, to a lower extent, by benzothiadiazoles and salicylic acid. Comparison between BBPK cell-free inhibition and in vivo PR-1 induction revealed that BBPK could be the target site of INA.
Pillonel
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Novartis Crop Protection Ltd, CH-4002 Basel, Switzerland. christian.pillonel@syngenta.com
D002614:Chelating Agents; D002846:Chromatography, Affinity / Q000379:methods*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D007113:Immunity, Innate; D007539:Isonicotinic Acids / Q000494:pharmacology; D010766:Phosphorylation; D010935:Plant Diseases; D047428:Protein Kinase Inhibitors; D011494:Protein Kinases / Q000302:isolation & purification* / Q000378:metabolism; D020156:Salicylic Acid / Q000378:metabolism; D012685:Sepharose; D013830:Thiadiazoles / Q000378:metabolism; D014026:Nicotiana / Q000201:enzymology*
D002614:Chelating Agents; D004791:Enzyme Inhibitors; D007539:Isonicotinic Acids; D047428:Protein Kinase Inhibitors; D013830:Thiadiazoles; C015700:benzo-1,2,3-thiadiazole; C077261:2,6-dichloroisonicotinic acid; D012685:Sepharose; D011494:Protein Kinases; D020156:Salicylic Acid