Upregulation of pigment epithelium-derived factor after laser photocoagulation.
OBJECTIVE
To determine the changes in the expression of pigment epithelium-derived factor in cultured human retinal pigment epithelial cells and rat retinas after laser photocoagulation.


METHODS
Experimental study of laser photocoagulation on human retinal pigment epithelial cells in culture and on adult rats. Reverse transcription-polymerase chain reaction and semiquantitative polymerase chain reaction analysis were used.


RESULTS
After photocoagulation, the mRNA expression of pigment epithelium-derived factor was upregulated in human retinal pigment epithelial cells at 6 hours and then gradually decreased. Compared with controls, significantly higher levels of pigment epithelium-derived factor were observed in rat retinas from 6 to 24 hours after laser photocoagulation (P <.005), and they were still higher than before photocoagulation at 2 weeks.


CONCLUSIONS
An upregulation of pigment epithelium-derived factor in retinal pigment epithelial cells and in the retina after photocoagulation suggests that pigment epithelium-derived factor plays a role in inhibiting neovascularization by its antiangiogenic activity.
Ogata
N
N
Department of Ophthalmology, Kansai Medical University, 10-15 Fumizono-cho, Moriguchi, Osaka 570-8507, Japan. ogata@takii.kmu.ac.jp
Tombran-Tink
J
J
Jo
N
N
Mrazek
D
D
Matsumura
M
M
D000818:Animals; D002478:Cells, Cultured; D005136:Eye Proteins; D006801:Humans; D017075:Laser Coagulation; D009414:Nerve Growth Factors; D010857:Pigment Epithelium of Eye / Q000166:cytology / Q000378:metabolism / Q000601:surgery*; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D012160:Retina / Q000378:metabolism / Q000601:surgery*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015843:Serpins / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation; D000099174:Pigment Epithelium-Derived Factor
D005136:Eye Proteins; D009414:Nerve Growth Factors; D011506:Proteins; D012333:RNA, Messenger; D015843:Serpins; D000099174:Pigment Epithelium-Derived Factor