Effects of cyclosporin A on cell fusion in a monkey kidney cell line persistently infected with measles virus.
The authors studied the effects of immunosuppressive peptide cyclosporin A (CsA) on cell fusion efficiency in cells persistently infected with measles virus (448-PI-Vero cells). Treatment of 448-PI-Vero cells with 5 microM CsA enhanced the infusion. In addition, the expression of measles virus antigen on cell surface was increased by treatment with CsA. The addition of phenothiazine, an anti-calmodulin drug, enhanced the fusion of 448-PI-Vero cells in the presence of CsA, although treatment with phenothiazine alone did not affect polykaryocyte formation. The enhancement of fusion efficiency in 448-PI-Vero cells by CsA was suppressed by oligopeptide Z-D-Phe-Phe-Gly, a synthetic oligopeptide that inhibits fusion induced by measles virus. Since the cell content of major virus-specific polypeptides, such as hemagglutinin, nucleoprotein or matrix protein is the same as in untreated controls, this fusion enhancement may be related to transport and accumulation of measles virus glycoproteins.
Watari
E
E
Department of Microbiology and Immunology, Nippon Medical School, Tokyo, Japan.
Shinya
E
E
Kurane
S
S
Takahashi
H
H
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D000998:Antiviral Agents / Q000494:pharmacology; D002470:Cell Survival; D002522:Chlorocebus aethiops; D016572:Cyclosporine / Q000494:pharmacology*; D004357:Drug Synergism; D015726:Giant Cells / Q000187:drug effects / Q000821:virology; D006389:Hemagglutinins, Viral / Q000032:analysis / Q000096:biosynthesis; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D008459:Measles virus / Q000187:drug effects*; D009842:Oligopeptides / Q000494:pharmacology; D010640:Phenothiazines / Q000494:pharmacology; D014709:Vero Cells / Q000187:drug effects*; D014779:Virus Replication / Q000187:drug effects
D000956:Antigens, Viral; D000998:Antiviral Agents; D006389:Hemagglutinins, Viral; D007166:Immunosuppressive Agents; D009842:Oligopeptides; D010640:Phenothiazines; C044920:phenylalanyl-phenylalanyl-glycine; D016572:Cyclosporine; C031637:phenothiazine