title: [Inhibition of gene expression by administration of homologous double-stranded RNA in Drosophila melanogaster cell culture].
abstract: Specific inhibition of gene expression by exogenous homologous double-stranded RNA (dsRNA) in invertebrates and in the early development of vertebrates is termed RNA interference. Cultured cells were cotransfected with reporter plasmids and dsRNA. The inhibitor effect on reporter gene expression depended on the extent of homology between dsRNA and the target gene. RNA interference was also studied in cells cotransfected with plasmids directing synthesis of sense and antisense RNAs. Production of antisense RNA only slightly inhibited expression of the reporter gene. Simultaneous expression of both sense and antisense RNAs from a special plasmid did not inhibit expression of the reporter construct.
authors.affiliation: Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, 123182 Russia.
authors.forename: A A
authors.initials: AA
authors.lastname: Aravin
authors.forename: V V
authors.initials: VV
authors.lastname: Vagin
authors.forename: Ia M
authors.initials: IaM
authors.lastname: Rozovskiĭ
authors.forename: V A
authors.initials: VA
authors.lastname: Gvozdev
mesh_terms: D000818:Animals; D004331:Drosophila melanogaster / Q000235:genetics*; D005786:Gene Expression Regulation; D017344:Genes, Insect; D017930:Genes, Reporter; D007763:Lac Operon; D010957:Plasmids; D016372:RNA, Antisense; D012330:RNA, Double-Stranded / Q000235:genetics*; D014162:Transfection
chemical_list: D016372:RNA, Antisense; D012330:RNA, Double-Stranded
vernacular_title: Podavlenie ékspressii genov vvedeniem gomologichnoĭ dvutsepochechnoĭ RNK v kul'ture kletok Drosophila melanogaster.