Structural elements of the osteopontin SVVYGLR motif important for the interaction with alpha(4) integrins.
The osteopontin SVVYGLR motif binds the integrins alpha(4)beta(1) and alpha(9)beta(1). We show that alpha(4)beta(7) also interacts with this motif and that an SVVYGLR-OH peptide antagonises the alpha(4)beta(7) MAdCAM interaction. The important elements of this motif required to bind alpha(4)beta(1) and alpha(4)beta(7) were probed using a series of mutated peptides based around SVVYGLR. Leu167 is important for the interaction with alpha(4) integrins, as is the C-terminal carboxylic acid of Arg168 exposed by thrombin cleavage. The importance of the acidic group means that SVVYGLR has structural elements in common with other alpha(4) integrin-binding motifs and suggests why thrombin cleavage activates this motif.
Green
P M
PM
Systems Research, GlaxoSmithKline Medicines Research Centre, Stevenage, UK.
Ludbrook
S B
SB
Miller
D D
DD
Horgan
C M
CM
Barry
S T
ST
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D015703:Antigens, CD / Q000378:metabolism*; D001665:Binding Sites; D002460:Cell Line; D006801:Humans; D039441:Integrin alpha4; D053495:Osteopontin; D011487:Protein Conformation; D012795:Sialoglycoproteins / Q000737:chemistry / Q000378:metabolism*
D015703:Antigens, CD; C506895:SPP1 protein, human; D012795:Sialoglycoproteins; D053495:Osteopontin; D039441:Integrin alpha4