title: Mechanical fluid flow and surfactant-TA influence activation of macrophages.
abstract: We examined the effect of mechanical fluid flow and surfactant on macrophage aggregation as the indication of macrophage activation. Mechanical fluid flow enhanced aggregation in phorbol myristate acetate (PMA)-treated human myeloid leukemic cell lines (HL-60 cells), but had no effect on differentiation of PMA-treated HL-60 cells. Surfactant-TA (an artificial surfactant) inhibited fluid flow-induced aggregation, but had no effect on differentiation of PMA-treated HL-60 cells. Human alveolar macrophages spontaneously formed small aggregates without stimulation. This aggregation was enhanced by fluid flow and inhibited by surfactant-TA. Taken together, these data suggest that macrophage activation is affected by fluid flow and surfactants.
authors.affiliation: First Department of Internal Medicine, Gunma University Faculty of Medicine, Maebashi, Japan. ymita@pop.showa.sb.gunma-u.ac.jp
authors.forename: Y
authors.initials: Y
authors.lastname: Mita
authors.forename: K
authors.initials: K
authors.lastname: Dobashi
authors.forename: T
authors.initials: T
authors.lastname: Nakazawa
authors.forename: M
authors.initials: M
authors.lastname: Mori
mesh_terms: D001688:Biological Products; D002454:Cell Differentiation; D018922:HL-60 Cells / Q000187:drug effects; D006801:Humans; D008262:Macrophage Activation / Q000502:physiology*; D016676:Macrophages, Alveolar / Q000166:cytology / Q000502:physiology*; D010812:Physical Stimulation; D011663:Pulmonary Surfactants / Q000494:pharmacology; D012212:Rheology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D001688:Biological Products; D011663:Pulmonary Surfactants; D013755:Tetradecanoylphorbol Acetate; C072197:beractant