RNA editing in higher plant plastids: oligoribonucleotide SSCP analysis allows the proof of base conversion directly at the RNA level.
Plastid RNA editing of a number of transcripts at specific sites changes genomically encoded cytidines to nucleosides, which act like uridines in RT-PCR analyses. To study plastid-editing directly at the RNA level, we established a single-strand conformational polymorphism assay for the discrimination of small RNA molecules. The electrophoretic mobility of a oligoribonucleotide resulting from a RNase T1-digested and edited plastid mRNA was shown to be identical with a control RNA molecule containing a uridine at the editing site, whereas the unedited RNA behaved like a RNA molecule containing a cytidine at the respective position.
Fuchs
M
M
GeneScan Europe AG, Freiburg, Germany.
Maier
R M
RM
Zeltz
P
P
D002874:Chromosome Mapping; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D018087:Plastids / Q000235:genetics*; D018807:Polymorphism, Single-Stranded Conformational; D017393:RNA Editing / Q000235:genetics*; D003313:Zea mays / Q000235:genetics
D017931:DNA Primers; D018076:DNA, Complementary