title: In vitro effects of anti-Trypanosoma cruzi compounds on HSP60 levels.
abstract: The effect of nifurtimox and 5-nitro-2-thienylmalononitrile (5NO2TM), a novel compound with anti-Trypanosoma cruzi activity, upon vitality and HSP60 immunoreactivity of epimastigotes, has been determined. Both products showed no activity against epimastigotes at 0.1 microg/ml, while at 0.5 and 1 microg/ml, after 24 h of incubation, densities of these groups were significantly reduced, when compared to controls. An enhancement of HSP60 immunoreactivity was observed after 24 h in groups treated with 0.5 and 1 microg/ml nifurtimox. On the other hand, 5NO2TM had no effect.
authors.affiliation: Departamento de Parasitología, Facultad de Farmacia, Universidad Complutense de Madrid, Ciudad Universitaria, Spain.
authors.forename: S
authors.initials: S
authors.lastname: Muelas-Serrano
authors.forename: J
authors.initials: J
authors.lastname: Pérez-Serrano
authors.forename: A
authors.initials: A
authors.lastname: Gómez-Barrio
authors.forename: C
authors.initials: C
authors.lastname: Ochoa
authors.forename: J
authors.initials: J
authors.lastname: Martínez
authors.forename: F
authors.initials: F
authors.lastname: Rodríguez-Caabeiro
mesh_terms: D000818:Animals; D014355:Chagas Disease / Q000469:parasitology; D018834:Chaperonin 60 / Q000187:drug effects / Q000378:metabolism*; D009547:Nifurtimox / Q000494:pharmacology*; D009574:Nitro Compounds / Q000494:pharmacology*; D021261:Parasitic Sensitivity Tests / Q000379:methods; D014344:Trypanocidal Agents / Q000494:pharmacology*; D014349:Trypanosoma cruzi / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism
chemical_list: D018834:Chaperonin 60; D009574:Nitro Compounds; D014344:Trypanocidal Agents; D009547:Nifurtimox