OsK2, a new selective inhibitor of Kv1.2 potassium channels purified from the venom of the scorpion Orthochirus scrobiculosus.
A novel inhibitor of voltage-gated K(+) channels has been purified to homogeneity from the venom of the black scorpion Orthochirus scrobiculosus. This toxin, named OsK2, has been characterized as a 28-residue peptide, containing six conserved cysteine residues and was shown to be a potent and selective blocker of Kv1.2 channels (K(d) = 97 nM). OsK2 is the second member of the 13th subfamily of short-chain K(+) channel-blocking peptides known thus far and is therefore called alpha-KTx 13.2.
Dudina
E E
EE
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Ul. Miklukho-Maklaya, 16/10, Moscow, 117997, GSP, Russia. dudinaee@azt.cardio.ru
Korolkova
Y V
YV
Bocharova
N E
NE
Koshelev
S G
SG
Egorov
T A
TA
Huys
I
I
Tytgat
J
J
Grishin
E V
EV
D000595:Amino Acid Sequence; D000818:Animals; D002845:Chromatography; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D003545:Cysteine / Q000737:chemistry; D018076:DNA, Complementary / Q000378:metabolism; D004175:Diptera; D004305:Dose-Response Relationship, Drug; D004594:Electrophysiology; D006110:Grasshoppers; D007700:Kinetics; D051663:Kv1.2 Potassium Channel; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009132:Muscles / Q000187:drug effects; D009474:Neurons / Q000187:drug effects; D009865:Oocytes / Q000378:metabolism; D010455:Peptides / Q000737:chemistry; D010957:Plasmids / Q000378:metabolism; D026902:Potassium Channel Blockers; D015221:Potassium Channels; D024642:Potassium Channels, Voltage-Gated; D012604:Scorpion Venoms / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D012605:Scorpions; D020539:Sequence Analysis, Protein; D017386:Sequence Homology, Amino Acid; D013997:Time Factors
D018076:DNA, Complementary; D051663:Kv1.2 Potassium Channel; C436512:OsK2 protein, Orthochirus scrobiculosus; D010455:Peptides; D026902:Potassium Channel Blockers; D015221:Potassium Channels; D024642:Potassium Channels, Voltage-Gated; D012604:Scorpion Venoms; D003545:Cysteine