title: UDP-sugar transporter implicated in glycosylation and processing of Notch.
abstract: Glycosylation modifies protein activities in various biological processes. Here, we report the functions of a novel UDP-sugar transporter (UST74C, an alternative name for Fringe connection (Frc)) localized to the Golgi apparatus in cellular signalling of Drosophila. Mutants in the frc gene exhibit phenotypes resembling wingless and Notch mutants. Both Fringe-dependent and Fringe-independent Notch pathways are affected, and both glycosylation and proteolytic maturation of Notch are defective in mutant larvae. The results suggest that changes in nucleotide-sugar levels can differently affect Wingless and two distinct aspects of Notch signalling.
authors.affiliation: Genetic Strain Research Center, National Institute of Genetics, Mishima, Shizuoka-ken 411-8540, Japan. sgoto@mrc-lmb.cam.ac.uk
authors.forename: S
authors.initials: S
authors.lastname: Goto
authors.forename: M
authors.initials: M
authors.lastname: Taniguchi
authors.forename: M
authors.initials: M
authors.lastname: Muraoka
authors.forename: H
authors.initials: H
authors.lastname: Toyoda
authors.forename: Y
authors.initials: Y
authors.lastname: Sado
authors.forename: M
authors.initials: M
authors.lastname: Kawakita
authors.forename: S
authors.initials: S
authors.lastname: Hayashi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D030801:Animals, Genetically Modified; D003433:Crosses, Genetic; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000196:embryology / Q000235:genetics* / Q000254:growth & development; D004625:Embryo, Nonmammalian / Q000502:physiology; D005260:Female; D006031:Glycosylation; D049452:Green Fluorescent Proteins; D006720:Homozygote; D007814:Larva; D008164:Luminescent Proteins / Q000032:analysis / Q000235:genetics; D008297:Male; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D016296:Mutagenesis; D010053:Ovary / Q000196:embryology; D051880:Receptors, Notch; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014539:Uridine Diphosphate Sugars / Q000378:metabolism*; D014921:Wings, Animal / Q000196:embryology
chemical_list: D029721:Drosophila Proteins; D008164:Luminescent Proteins; D008565:Membrane Proteins; C497759:N protein, Drosophila; D051880:Receptors, Notch; D014539:Uridine Diphosphate Sugars; D049452:Green Fluorescent Proteins