title: [Effects of tumor necrosis factor alpha on the expression of connective tissue growth factor in hepatic stellate cells].
abstract: OBJECTIVE
  To investigate the effect of tumor necrosis factor alpha (TNF-alpha) on the expression of connective tissue growth factor (CTGF) in rat hepatic stellate cells (HSC) in vitro.
  
  
  METHODS
  HSCs were prepared from SD rats by in situ perfusion and single-step Nycodenz gradient, and were treated with TNF-alpha and TGF-beta 1. The expression of CTGF in HSC was assessed using reverse transcription-polymerase chain reaction (RT-PCR).
  
  
  RESULTS
  Both TNF-alpha and TGF-beta 1 could stimulate the expression of CTGF mRNA in HSC. The expression of CTGF was not found in HSC after treatment with TNF-alpha(10microgram/L) for 6 hours, but was observed after TNF-alpha treatment for 24 and 48 hours. TGF-beta1 could induce the mRNA expression for CTGF just after 6 hours of treatment at 1microgram/L concentration.
  
  
  CONCLUSIONS
  TNF-alpha is able to induce the expression of CTGF in HSC, and this may be one of the mechanisms for TNF-alpha involving in the liver fibrosis.
authors.affiliation: Department of Internal Medicine, First Affiliated Hospital, West China University of Medical Sciences, Chengdu 610041, China.
authors.forename: X
authors.initials: X
authors.lastname: Liu
authors.forename: H
authors.initials: H
authors.lastname: Wu
authors.forename: F
authors.initials: F
authors.lastname: Liu
authors.forename: M
authors.initials: M
authors.lastname: Huang
authors.forename: O
authors.initials: O
authors.lastname: Qiang
authors.forename: S
authors.initials: S
authors.lastname: Huang
mesh_terms: D000818:Animals; D055513:Connective Tissue Growth Factor; D006133:Growth Substances / Q000096:biosynthesis* / Q000235:genetics; D017874:Immediate-Early Proteins / Q000096:biosynthesis* / Q000235:genetics; D036341:Intercellular Signaling Peptides and Proteins; D008099:Liver / Q000166:cytology* / Q000378:metabolism*; D008103:Liver Cirrhosis / Q000209:etiology; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D016212:Transforming Growth Factor beta / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
chemical_list: C526316:CCN2 protein, rat; D006133:Growth Substances; D017874:Immediate-Early Proteins; D036341:Intercellular Signaling Peptides and Proteins; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; D014409:Tumor Necrosis Factor-alpha; D055513:Connective Tissue Growth Factor