title: [Estradiol Reference Standard (Control 001) of National Institute of Health Sciences].
abstract: The raw material of tocopherol was tested for the preparation of "Estradiol Reference Standard (Control 991)". Analytical data obtained were: melting point, 178.5 degrees C: UV spectrum, lambda max of 281 nm and specific absorbance in ethanol at lambda max = 77.7; IR spectrum, same as that of the Estradiol Reference Standard (Control 964); optical rotation, [alpha]D20 = +79.5 degrees; thin-layer chromatography, one impurity was detected; high-performance liquid chromatography (HPLC), total amount of impurities estimated to be less than 0.6%; loss on drying, 3.3%; assay, 100.2% by UV spectrophotometry and 99.9% by HPLC. Based on the above results, the raw material was authorized as the Estradiol Reference Standard (Control 001) of the National Institute of Health Sciences.
authors.forename: M
authors.initials: M
authors.lastname: Iwata
authors.forename: T
authors.initials: T
authors.lastname: Koide
authors.forename: K
authors.initials: K
authors.lastname: Maekawa
authors.forename: H
authors.initials: H
authors.lastname: Saito
authors.forename: T
authors.initials: T
authors.lastname: Tanimoto
authors.forename: S
authors.initials: S
authors.lastname: Okada
mesh_terms: D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D004958:Estradiol / Q000032:analysis / Q000592:standards*; D006077:Government Agencies; D007564:Japan; D012015:Reference Standards
chemical_list: D004958:Estradiol