Holger Hydén's technique of preparation of single Deiters' neurons and study of permeability characteristics of their plasma membranes.
The protocols described here refer to Hydén's technique of isolation and microdissection of vestibular Deiters' neurons from adult mammals. The isolation of Deiters' cells from bovine is described and an example is given of the immunocytochemical visualization of their GABA(A) receptors by monoclonal antibodies against the beta(2/3) subunit. In addition, the protocol of the method for isolation of Deiters' cells from adult rabbit brain stem, the preparation of their plasma membranes and the study of their permeability characteristics is presented. Also in this case, examples of its application to the determination of chloride permeability and its modulation by GABA are given.
Rapallino
M V
MV
Centro di Neurofisiologia Cerebrale, C.N.R., Via De Toni 5, 16132, Genova, Italy. mrapal@neurologia.unige.it
Cupello
A
A
D000818:Animals; D002417:Cattle; D002463:Cell Membrane Permeability; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods*; D002712:Chlorides / Q000378:metabolism; D004867:Equipment Design; D008297:Male; D009474:Neurons / Q000378:metabolism*; D009488:Neurosciences / Q000295:instrumentation / Q000379:methods*; D020033:Protein Isoforms / Q000378:metabolism; D011817:Rabbits; D011963:Receptors, GABA-A / Q000378:metabolism; D014726:Vestibular Nuclei / Q000166:cytology / Q000378:metabolism*
D002712:Chlorides; D020033:Protein Isoforms; D011963:Receptors, GABA-A