Dendritic cells transduced with HIV Nef express normal levels of HLA-A and HLA-B class I molecules.
HIV Nef protein is important for viral pathogenesis and disease progression. Nef downregulates CD4 and major histocompatibility antigens on the surface of HIV-infected T cells. HIV also infects dendritic cells. We wanted to determine if Nef had a similar function in professional antigen-presenting cells, where downregulation of Class I could have important effects on the initiation of HIV specific cytolytic T cell responses. We infected human dendritic cells with adenovirus expressing Nef. In contrast to T cells and Hela cells, HLA-A and HLA-B molecules are not downregulated nor are other class I molecules increased. We show that, in dendritic cells, HIV Nef has little effect on CD4 or Class I expression.
Cramer
L A
LA
Department of Microbiology and Immunology, University of North Carolina, Chapel Hill, NC 27599-7290, USA.
Frelinger
J A
JA
D000256:Adenoviridae / Q000235:genetics* / Q000378:metabolism; D015704:CD4 Antigens / Q000378:metabolism; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism / Q000821:virology; D015693:Gene Products, nef / Q000378:metabolism*; D015754:Genes, nef; D049452:Green Fluorescent Proteins; D015497:HIV-1 / Q000378:metabolism; D015234:HLA-A Antigens / Q000378:metabolism*; D015235:HLA-B Antigens / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D014161:Transduction, Genetic; D054311:nef Gene Products, Human Immunodeficiency Virus
D015704:CD4 Antigens; D015693:Gene Products, nef; D015234:HLA-A Antigens; D015235:HLA-B Antigens; D008164:Luminescent Proteins; D054311:nef Gene Products, Human Immunodeficiency Virus; D049452:Green Fluorescent Proteins