title: Effect of 17 beta-estradiol on the brain damage and metabolic changes in rats.
abstract: An in vivo model of glutamate excitotoxicity in which glutamate is applied to the cortex of rats through a microdialysis probe has been used to investigate the neuroprotective processes initiated by 17 beta-estradiol. Rats were pre-treated with 17 beta-estradiol i.v. before local application of glutamate. The experimental results showed that pre-treatment with 17 beta-estradiol significantly reduced the size of the glutamate-induced lesion. In the microdialysates, the peak of lactate observed immediately after glutamate application was significantly higher and longer lasting after 17 beta-estradiol pre-treatment. The level of extracellular glucose was markedly decreased concomitantly to the increase in lactate, but no difference could be observed with and without 17 beta-estradiol pre-treatment. These suggest a new neuroprotective mechanism of 17 beta-estradiol by activating glutamate-induced lactate production. This effect on lactate production and lesion reduction is estrogen receptor dependent and is abolished totally by estrogen antagonist tamoxifen. It was also demonstrated here that high lactate subserves estrogen neuroprotection during glutamate toxicity.
authors.affiliation: Department of Neurosurgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030.
authors.forename: J
authors.initials: J
authors.lastname: Chen
authors.forename: T
authors.initials: T
authors.lastname: Lei
authors.forename: M F
authors.initials: MF
authors.lastname: Ritz
authors.forename: A
authors.initials: A
authors.lastname: Mendelowitsch
mesh_terms: D000818:Animals; D001927:Brain Diseases / Q000139:chemically induced / Q000378:metabolism*; D004958:Estradiol / Q000494:pharmacology*; D018698:Glutamic Acid; D008297:Male; D017551:Microdialysis; D018696:Neuroprotective Agents / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D018696:Neuroprotective Agents; D018698:Glutamic Acid; D004958:Estradiol