[Effects of vascular endothelial growth factor on adhesion molecular expression of hepatocellular carcinoma HepG].
OBJECTIVE
To study the effect of vascular endothelial growth factor (VEGF) on homotypic adhesion and E-cadherin of hepatocellular carcinoma cells.


METHODS
We measured the function of homotypic adhesion with (3)H-TdR infiltration and the expression of E-cadherin with laser scanning confocal microscope.


RESULTS
After 60, 90, 120min, the values (dpm/min) of (3)H-TdR infiltration in HepG(2) cells induced by 1ng/ml, 5ng/ml VEGF were 1758.67+/-289.46, 1380.03+/-328.55, 2657.43 +/- 310.31 and 3124.30+/-262.14, 2245.60+/-273.24, 2091.52+/-213.84. The values by 10ng/ml VEGF were 1232.32+/-201.04, 2337.50+/-333.04, 2236.99 +/- 237.07, which were significantly lower than those in control group (P<0.05 or 0.01). The expression of E-cadherin in hepatocellular carcinoma cells induced by 5ng/ml VEGF was 352+/-56 and much lower than that in control group (757+/-103, P<0.01).


CONCLUSIONS
VEGF can decrease homotopic adhesion of hepatocellular carcinoma HepG(2) cell, which is connected with the less expression of E-cadherin in hepatocellular carcinoma HepG(2) cell.
Mao
H
H
Institute of Digestive Diseases of Nanfang Hospital, First Military Medical University, Guangzhou 510515, China.
Lai
Z
Z
Zhao
M
M
Yuan
A
A
Zhou
D
D
D015820:Cadherins / Q000096:biosynthesis*; D006528:Carcinoma, Hepatocellular / Q000473:pathology*; D002448:Cell Adhesion / Q000187:drug effects; D016228:Endothelial Growth Factors / Q000494:pharmacology*; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology*; D008222:Lymphokines / Q000494:pharmacology*; D014407:Tumor Cells, Cultured; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
D015820:Cadherins; D016228:Endothelial Growth Factors; D008222:Lymphokines; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors