title: Tissue dynamics of CD8 lymphocytes that suppress viral replication in cats infected neonatally with feline immunodeficiency virus.
abstract: The purpose of this study was to determine the tissue distribution and antiviral activity of the CD8 lymphocytes that suppress the replication of feline immunodeficiency virus (FIV). Cell-associated FIV load, CD8alpha(+)beta(low) cells, and CD8 cell-mediated suppression of FIV were measured serially in the blood, thymus, and peripheral lymph nodes after neonatal inoculation. Between 6 and 10 weeks, relative numbers of CD8alpha(+)beta(low) cells increased, whereas CD8alpha(+)beta(high) cells declined in the thymus and blood of infected cats. By 12-16 weeks, the lymph nodes were enlarged because of an absolute expansion of all CD8beta subpopulations. The strength of CD8 cell-mediated FIV suppression in vitro, but not CD8alpha(+)beta(low) cell content, was correlated inversely with virus load in the thymus and blood. Thus, after neonatal FIV inoculation, CD8alpha(+)beta(low) cells first occupy the thymus and blood, where strong CD8 cell-mediated antiviral activity is linked to reduced virus load in multiple lymphoid tissues.
authors.affiliation: Department of Pathobiology, College of Veterinary Medicine, University of Florida, Gainesville, 32610-0880, USA.
authors.forename: P C
authors.initials: PC
authors.lastname: Crawford
authors.forename: G P
authors.initials: GP
authors.lastname: Papadi
authors.forename: J K
authors.initials: JK
authors.lastname: Levy
authors.forename: N A
authors.initials: NA
authors.lastname: Benson
authors.forename: A
authors.initials: A
authors.lastname: Mergia
authors.forename: C M
authors.initials: CM
authors.lastname: Johnson
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000821:virology; D002415:Cats; D002478:Cells, Cultured; D016181:Feline Acquired Immunodeficiency Syndrome / Q000276:immunology*; D005434:Flow Cytometry; D016089:Immunodeficiency Virus, Feline / Q000276:immunology / Q000302:isolation & purification / Q000502:physiology*; D008198:Lymph Nodes / Q000276:immunology / Q000821:virology; D012044:Regression Analysis; D016176:T-Lymphocyte Subsets / Q000276:immunology; D013950:Thymus Gland / Q000276:immunology / Q000821:virology; D013997:Time Factors; D014766:Viremia / Q000276:immunology; D014779:Virus Replication / Q000276:immunology*