title: Increased serum IgE in alcoholics: relationship with Th1/Th2 cytokine production by stimulated blood mononuclear cells.
abstract: BACKGROUND
  Increased serum immunoglobulin (Ig) E values are frequently found in alcoholics. Cytokines produced by T-helper-2 (Th2) lymphocytes are required for IgE synthesis. Chronic alcoholism is associated with altered cytokine balance. This study analyzed the relationship between Th1 and Th2 cytokine production by stimulated peripheral blood mononuclear cells (PBMCs) and serum IgE levels, both in atopic and nonatopic alcoholics.
  
  
  METHODS
  Twenty-five patients admitted to the hospital with alcohol withdrawal syndrome were included in the study. Five were classified as atopic and 20 as nonatopic by means of skin-prick tests. Interleukin-4 (IL-4), IL-10, IL-12, IL-13, and interferon gamma were measured in the supernatants of 48-hr cultures of PBMCs stimulated with phytohemagglutinin. Total serum IgE was measured by chemiluminescent enzyme immunoassay. Results were compared with those of 15 healthy controls (seven atopics and eight nonatopics).
  
  
  RESULTS
  Total serum IgE concentrations were higher in alcoholics than in controls, in both atopic and nonatopic subjects. The ratio of IL-4 to interferon gamma production by phytohemagglutinin-stimulated PBMCs (as an approach to Th2/Th1 balance) was significantly lower in alcoholics than in healthy controls, both in the atopic and in the nonatopic group. No difference was observed regarding IL-10, IL-12, and IL-13 production between alcoholics and controls. No correlation was demonstrated between cytokine production and total serum IgE levels in any group.
  
  
  CONCLUSIONS
  Increased total serum IgE is observed in alcoholics together with a paradoxically low ratio of Th2 to Th1 cytokine production by phytohemagglutinin-stimulated PBMCs. These findings are independent of the atopic status of patients.
authors.affiliation: Division of Internal Medicine, Complejo Hospitalario Universitario, Santiago de Compostela, Spain.
authors.forename: M J
authors.initials: MJ
authors.lastname: Domínguez-Santalla
authors.forename: C
authors.initials: C
authors.lastname: Vidal
authors.forename: J
authors.initials: J
authors.lastname: Viñuela
authors.forename: L F
authors.initials: LF
authors.lastname: Pérez
authors.forename: A
authors.initials: A
authors.lastname: González-Quintela
mesh_terms: D000328:Adult; D000368:Aged; D000437:Alcoholism / Q000276:immunology* / Q000503:physiopathology; D016207:Cytokines / Q000096:biosynthesis*; D005243:Feces / Q000469:parasitology; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D007070:Immunoglobulin A / Q000097:blood; D007073:Immunoglobulin E / Q000097:blood*; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D007371:Interferon-gamma / Q000096:biosynthesis; D016753:Interleukin-10 / Q000096:biosynthesis; D018664:Interleukin-12 / Q000096:biosynthesis; D018793:Interleukin-13 / Q000096:biosynthesis; D015847:Interleukin-4 / Q000096:biosynthesis; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008099:Liver / Q000503:physiopathology; D008163:Luminescent Measurements; D016131:Lymphocyte Subsets; D008297:Male; D008875:Middle Aged; D009752:Nutritional Status; D010835:Phytohemagglutinins / Q000494:pharmacology; D013375:Substance Withdrawal Syndrome / Q000276:immunology
chemical_list: D016207:Cytokines; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D018793:Interleukin-13; D010835:Phytohemagglutinins; D016753:Interleukin-10; D018664:Interleukin-12; D015847:Interleukin-4; D007073:Immunoglobulin E; D007371:Interferon-gamma