Functionalized xenon as a biosensor.
The detection of biological molecules and their interactions is a significant component of modern biomedical research. In current biosensor technologies, simultaneous detection is limited to a small number of analytes by the spectral overlap of their signals. We have developed an NMR-based xenon biosensor that capitalizes on the enhanced signal-to-noise, spectral simplicity, and chemical-shift sensitivity of laser-polarized xenon to detect specific biomolecules at the level of tens of nanomoles. We present results using xenon "functionalized" by a biotin-modified supramolecular cage to detect biotin-avidin binding. This biosensor methodology can be extended to a multiplexing assay for multiple analytes.
Spence
M M
MM
Department of Chemistry, University of California, Berkeley, CA 94720, USA.
Rubin
S M
SM
Dimitrov
I E
IE
Ruiz
E J
EJ
Wemmer
D E
DE
Pines
A
A
Yao
S Q
SQ
Tian
F
F
Schultz
P G
PG
D001360:Avidin / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D001710:Biotin / Q000737:chemistry; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D011506:Proteins / Q000737:chemistry*; D014978:Xenon
D011506:Proteins; D001360:Avidin; D014978:Xenon; D001710:Biotin