Accurate measurement of small spin-spin couplings in partially aligned molecules using a novel J-mismatch compensated spin-state-selection filter.
The accurate measurement of small spin-spin coupling constants in macromolecules dissolved in a liquid crystalline phase is important in the context of molecular structure investigation by modern liquid state NMR. A new spin-state-selection filter, DIPSAP, is presented with significantly reduced sensitivity to J-mismatch of the filter delays compared to previously proposed pulse sequences. DIPSAP presents an attractive new approach for the accurate measurement of small spin-spin coupling constants in molecules dissolved in anisotropic solution. Application to the measurement of 15N-13C' and 1H(N)-13C' coupling constants in the peptide planes of 13C, 15N labeled proteins demonstrates the high accuracy obtained by a DIPSAP-based experiment.
Brutscher
B
B
Institut de Biologie Structurale, Jean-Pierre Ebel C.N.R.S.-C.E.A., 41, rue Jules Horowitz, 38027 Grenoble Cedex, France.
D002244:Carbon / Q000737:chemistry; D002247:Carbon Isotopes; D006859:Hydrogen / Q000737:chemistry; D006860:Hydrogen Bonding; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008433:Mathematics; D009584:Nitrogen / Q000737:chemistry; D009587:Nitrogen Isotopes; D011506:Proteins / Q000737:chemistry*
D002247:Carbon Isotopes; D009587:Nitrogen Isotopes; D011506:Proteins; D002244:Carbon; D006859:Hydrogen; D009584:Nitrogen