title: Promoter sequences regulated by the calcineurin-activated transcription factor Crz1 in the yeast ENA1 gene.
abstract: In Saccharomyces cerevisiae the transcription of the ENA1 gene is modulated by multiple transduction pathways that respond to osmotic, ionic and nutrient stresses. We have investigated the molecular mechanisms involved in ENA1 induction by the calcium-calcineurin-activated transcription factor Crzl/Tcn1. We found in the ENA1 promoter a calcium-responsive, Crzl-dependent upstream activating region (UASENA1) located between -713 bp and 826 bp relative to the translation start. This region contains two separate control elements: the upstream element (5'-GAATGGCTG-3') between -813 and -821 binds Crzlp with lower affinity and mostly contributes to basal ENA1 expression, whereas the downstream element (5'-GGGTGGCTG-3') between 727 and 719 binds Crz1p with higher affinity and is a major determinant of the induction response to calcium.
authors.affiliation: Universidad del País Vasco, Facultad de Ciencias Químicas, Departamento de Química Aplicada, San Sebastián, Spain.
authors.forename: I
authors.initials: I
authors.lastname: Mendizabal
authors.forename: A
authors.initials: A
authors.lastname: Pascual-Ahuir
authors.forename: R
authors.initials: R
authors.lastname: Serrano
authors.forename: I F
authors.initials: IF
authors.lastname: de Larrinoa
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics*; D001483:Base Sequence; D019703:Calcineurin / Q000235:genetics; D027682:Cation Transport Proteins; D004268:DNA-Binding Proteins; D015966:Gene Expression Regulation, Fungal; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D000254:Sodium-Potassium-Exchanging ATPase; D015534:Trans-Activators / Q000235:genetics*; D014157:Transcription Factors; D015533:Transcriptional Activation
chemical_list: C109868:CRZ1 protein, S cerevisiae; D027682:Cation Transport Proteins; D004268:DNA-Binding Proteins; C086375:ENA1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D015534:Trans-Activators; D014157:Transcription Factors; D019703:Calcineurin; D000251:Adenosine Triphosphatases; D000254:Sodium-Potassium-Exchanging ATPase