Functional analysis of the Saccharomyces cerevisiae YFR021w/YGR223c/YPL100w ORF family suggests relations to mitochondrial/peroxisomal functions and amino acid signalling pathways.
Saccharomyces cerevisiae YFR021w, YGR223c and YPL100w are paralogous ORFs of unknown function. Phenotypic analysis of overexpression, single-, double- and triple-ORF deletion strains under various growth conditions indicated mitochondria-related functions for all three ORFs. Two-hybrid screens of a yeast genomic library identified potentially interacting proteins for the three ORFs. Among these, the transcriptional activator Rtg3p interacted with both Yfr021wp and Ypl100wp and both ORF single deletions reduced the constitutive expression of the RTG-regulated CIT2 and DLD3 genes and caused typical retrograde response of CIT2 and DLD3 under growth conditions requiring functional mitochondria, indicating that YFR021w and YPL100w are also involved in unidentified mitochondrial functions. Ptr3p, a component of the amino acid sensor Ssy1p/Ptr3p, was also found as a two-hybrid interactant of Yfr021wp. Of the three single-ORF deletions, ypl100w Delta exhibited ptr3 Delta-similar phenotypes. These findings, combined with the fact that RTG-dependent expression is modulated by specific amino acids, suggested possible relations of Yfr021wp and Ypl100wp to amino acid signalling pathways. Under most conditions examined, the effects of the single- and double-ORF deletions indicated that YFR021w, YPL100w and YGR223c are not parts of the same pathway. We found no unique phenotype attributed to the deletion of YGR223c. However, its function interferes with the function of the other two ORFs, as revealed by the effects of double- and triple-ORF deletions.
Georgakopoulos
T
T
Foundation for Research and Technology-HELLAS, Institute of Molecular Biology and Biotechnology, PO Box 1527, Heraklion 711 10, Crete, Greece.
Koutroubas
G
G
Vakonakis
I
I
Tzermia
M
M
Prokova
V
V
Voutsina
A
A
Alexandraki
D
D
D000595:Amino Acid Sequence; D015966:Gene Expression Regulation, Fungal; D008928:Mitochondria / Q000502:physiology; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional / Q000235:genetics / Q000502:physiology; D016366:Open Reading Frames / Q000235:genetics / Q000502:physiology* / Q000528:radiation effects; D020675:Peroxisomes / Q000502:physiology; D010641:Phenotype; D010957:Plasmids / Q000235:genetics / Q000502:physiology; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D020798:Two-Hybrid System Techniques