Isolation and characterization of a species-specific DNA fragment for identification of Candida (Torulopsis) glabrata by PCR.
A PCR specific for Candida glabrata that amplifies a mitochondrial rRNA gene fragment was developed by analysis of C. glabrata-specific agarose gel bands, which were generated by arbitrarily primed PCR. The expected PCR product was successfully amplified with genomic DNA from 95 C. glabrata isolates but not from a number of other fungal isolates.
Becker
K
K
Institute of Medical Microbiology, University of Münster, D-48149 Münster, Germany. kbecker@uni-muenster.de
Badehorn
D
D
Keller
B
B
Schulte
M
M
Böhm
K H
KH
Peters
G
G
Fegeler
W
W
D002175:Candida / Q000145:classification* / Q000235:genetics*; D017931:DNA Primers; D004271:DNA, Fungal / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013045:Species Specificity
D017931:DNA Primers; D004271:DNA, Fungal