Hexuronyl C5-epimerases in alginate and glycosaminoglycan biosynthesis.
The sugar residues in most polysaccharides are incorporated as their corresponding monomers during polymerization. Here we summarize the three known exceptions to this rule, involving the biosynthesis of alginate, and the glycosaminoglycans, heparin/heparan sulfate and dermatan sulfate. Alginate is synthesized by brown seaweeds and certain bacteria, while glycosaminoglycans are produced by most animal species. In all cases one of the incorporated sugar monomers are being C5-epimerized at the polymer level, from D-mannuronic acid to L-guluronic acid in alginate, and from D-glucuronic acid to L-iduronic acid in glycosaminoglycans. Alginate epimerization modulates the mechanical properties of seaweed tissues, whereas in bacteria it seems to serve a wide range of purposes. The conformational flexibility of iduronic acid units in glycosaminoglycans promotes apposition to, and thus functional interactions with a variety of proteins at cell surfaces and in the extracellular matrix. In the bacterium Azotobacter vinelandii the alginates are being epimerized at the cell surface or in the extracellular environment by a family of evolutionary strongly related modular type and Ca(2+)-dependent epimerases (AlgE1-7). Each of these enzymes introduces a specific distribution pattern of guluronic acid residues along the polymer chains, explaining the wide structural variability observed in alginates isolated from nature. Glycosaminoglycans are synthesized in the Golgi system, through a series of reactions that include the C5-epimerization reaction along with extensive sulfation of the polymers. The single, Ca(2+)-independent, epimerase in heparin/heparan sulfate biosynthesis and the Ca(2+)-dependent dermatan sulfate epimerase(s) also generate variable epimerization patterns, depending on other polymer-modification reactions. The alginate and heparin epimerases appear unrelated at the amino acid sequence level, and have probably evolved through independent evolutionary pathways; however, hydrophobic cluster analysis indicates limited similarity. Seaweed alginates are widely used in industry, while heparin is well established in the clinic as an anticoagulant.
Valla
S
S
Department of Biotechnology, The Norwegian University of Science and Technology, 7491 Trondheim, Norway.
Li
J
J
Ertesvåg
H
H
Barbeyron
T
T
Lindahl
U
U
D000464:Alginates / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D002238:Carbohydrate Epimerases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D002240:Carbohydrate Sequence; D020723:Glucuronic Acid; D006025:Glycosaminoglycans / Q000096:biosynthesis* / Q000502:physiology; D006603:Hexuronic Acids; D008969:Molecular Sequence Data; D013329:Structure-Activity Relationship
D000464:Alginates; D006025:Glycosaminoglycans; D006603:Hexuronic Acids; D020723:Glucuronic Acid; D002238:Carbohydrate Epimerases