Cloning and nucleotide sequence of the mycodextranase gene from Streptomyces sp. J-13-3.
Mycodextranase (EC 3.2.1.61) is an alpha-glucanase that cleaves alpha-1,4-bonds of alternating alpha-1,3- and alpha-1,4-linked D-glucan (nigeran). The gene encoding mycodextranase from Streptomyces sp. J-13-3 was cloned by hybridization with a degenerate oligonucleotide probe from the amino-terminal amino acid sequence of the enzyme and its nucleotide structure was analyzed. The open reading frame consisted of 1,803 base pairs encoding a signal peptide of 60 amino acids and a mature protein of 540 amino acids with a calculated molecular weight of 56,078. The deduced amino acid sequence showed weak similality to a chitinase homolog from Streptomyces lividans and a chitinase from Xanthomonas sp.
Okazaki
K
K
Department of Life Sciences, Faculty of Agriculture, Kagawa University, Miki, Japan. okazaki@ag.kagawa-u.ac.jp
Amano
T
T
Morimoto
T
T
Iemoto
T
T
Kawabata
T
T
Hayakawa
S
S
Akimitsu
K
K
D000595:Amino Acid Sequence; D001483:Base Sequence; D002240:Carbohydrate Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D005936:Glucans / Q000737:chemistry / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D013302:Streptomyces / Q000201:enzymology* / Q000235:genetics*
D004269:DNA, Bacterial; D005936:Glucans; C001290:glucan nigeran; D006026:Glycoside Hydrolases; C021817:mycodextranase