Extraction of lipid membrane incorporated vitamin E by sucrose polyesters.
2H and 31P solid state NMR have been used to probe, at the molecular level, the interaction between structurally different sucrose polyesters and a phospholipid membrane into which alpha-tocopherol and specifically deuterated alpha-[5,7-(2)H(6)] tocopherol has been incorporated. Our results show that at high concentration (>or=10 mol%) sucrose octapalmitate (SOP) and sucrose hexapalmitate (SHxP) deplete bilayer-associated alpha-tocopherol in dipalmitoyl phosphatidalcholine (DPPC) multilamellar dispersions and preferentially sequester the alpha-tocopherol into a fluid sucrose polyesters (SPE) phase located proximal to the membrane surface. It is demonstrated that the ability of SPEs to function as a 'lipophilic sink' depends strongly on sucrose polyester concentration and degree of esterification.
McManus
G G
GG
Institute for Biological Sciences, National Research Council of Canada, Ottawa, Ont., Canada K1A 0R6.
Buchanan
G W
GW
Jarrell
H C
HC
D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry*; D003903:Deuterium; D008051:Lipid Bilayers / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008954:Models, Biological; D008968:Molecular Conformation; D010168:Palmitates / Q000737:chemistry*; D013499:Surface Properties; D014810:Vitamin E / Q000737:chemistry* / Q000302:isolation & purification*
D008051:Lipid Bilayers; D008081:Liposomes; D010168:Palmitates; D014810:Vitamin E; D015060:1,2-Dipalmitoylphosphatidylcholine; D003903:Deuterium