title: Immunization with Haemophilus influenzae type b-CRM(197) conjugate vaccine elicits a mixed Th1 and Th2 CD(4+) T cell cytokine response that correlates with the isotype of antipolysaccharide antibody.
abstract: Haemophilus influenzae type b (Hib) capsular polysaccharide (PS) induces protective antibodies but is T independent and poorly immunogenic in infants. Conjugate vaccines of Hib PS linked to proteins, such as CRM(197), increase the PS antibody titer and elicit immunologic memory. To define the conjugate-induced memory T cell response, 19 adults were immunized with Hib-CRM(197), and antibody titers, carrier protein-specific CD4(+) T cell proliferation, and cytokine production were measured. Hib-CRM(197) induced PS and CRM(197) antibodies, vigorous T cell recall responses, and production of cytokines, including interleukin (IL)-2, IL-5, IL-10, and interferon-gamma. There was marked variability in PS antibody titer, despite consistent CRM(197)-specific recall responsiveness, which correlated with peak IgM and IgA PS antibody titers. Correlations were also found between IL-2 and IL-5 and IgA PS antibody levels. Hib-CRM(197) induced a rapid increase in CRM(197)-specific memory T cells and mixed Th1/Th2 cytokines, which may regulate the isotype and quantity of PS antibody.
authors.affiliation: Department of Pediatrics, Case Western Reserve University, Cleveland, Ohio, USA.
authors.forename: K K
authors.initials: KK
authors.lastname: Kamboj
authors.forename: C L
authors.initials: CL
authors.lastname: King
authors.forename: N S
authors.initials: NS
authors.lastname: Greenspan
authors.forename: H L
authors.initials: HL
authors.lastname: Kirchner
authors.forename: J R
authors.initials: JR
authors.lastname: Schreiber
mesh_terms: D000328:Adult; D000907:Antibodies, Bacterial / Q000097:blood*; D016667:Bacterial Capsules; D001426:Bacterial Proteins / Q000008:administration & dosage* / Q000276:immunology; D016207:Cytokines / Q000097:blood*; D006192:Haemophilus Infections / Q000276:immunology / Q000517:prevention & control*; D018073:Haemophilus Vaccines / Q000008:administration & dosage* / Q000276:immunology; D019741:Haemophilus influenzae type b / Q000276:immunology; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D007156:Immunologic Memory; D007279:Injections, Subcutaneous; D007371:Interferon-gamma / Q000097:blood; D007378:Interleukins / Q000097:blood; D011135:Polysaccharides, Bacterial / Q000008:administration & dosage* / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology*; D018418:Th2 Cells / Q000276:immunology*; D014611:Vaccination; D018074:Vaccines, Conjugate / Q000008:administration & dosage / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D016207:Cytokines; D018073:Haemophilus Vaccines; C044237:Haemophilus influenzae type b polysaccharide vaccine; D007070:Immunoglobulin A; D007075:Immunoglobulin M; D007378:Interleukins; D011135:Polysaccharides, Bacterial; D018074:Vaccines, Conjugate; C028581:CRM197 (non-toxic variant of diphtheria toxin); D007371:Interferon-gamma