Sodium sulfite and N-acetylcysteine: new additives to dialysate for inhibiting formation of glucose degradation products and advanced glycation end-products.
The present study evaluated the inhibiting effect of various chemicals on the advanced glycation end-product (AGEs) cross-linking caused in protein by glucose degradation products (GDPs). We evaluated a few dozen organic and inorganic chemicals--in addition to previously reported AGE inhibitors, such as thiazolium derivatives and aminoguanidine--for their inhibiting effect. Collagen IV (from human placenta) or human serum albumin (HSA) was incubated with an AGE accelerator and one of the selected chemicals in phosphate buffer solution at 37 degrees C for as long as 14 days. Fluorescence intensity (440 nm) was determined after a given incubation time. Among 36 chemicals tested, 8 new chemicals and 5 previously known AGE inhibitors significantly suppressed the increase in fluorescence intensity seen after incubation of HSA with methylglyoxal. We believe that 6 chemicals may effectively quench GDPs and inhibit AGE cross-link formation, in a manner different from that of aminoguanidine and thiazolium.
Sakai
A
A
Peritoneal Dialysis Research Project, Japan Science and Technology Corporation, c/o Tokyo Metropolitan Industrial Technology Research Institute, 11-1 Fukazawa 2-chome, Setagaya-ku, Tokyo 158-0081, Japan.
Nakayama
M
M
Numata
M
M
Takesawa
S
S
Nakamoto
M
M
D000111:Acetylcysteine; D024141:Collagen Type IV; D003847:Deoxyglucose / Q000031:analogs & derivatives*; D015314:Dialysis Solutions / Q000737:chemistry*; D005453:Fluorescence; D005947:Glucose / Q000737:chemistry*; D017127:Glycation End Products, Advanced / Q000037:antagonists & inhibitors / Q000737:chemistry*; D006038:Glyoxylates; D006801:Humans; D066298:In Vitro Techniques; D011765:Pyruvaldehyde; D012709:Serum Albumin; D013447:Sulfites
D024141:Collagen Type IV; D015314:Dialysis Solutions; D017127:Glycation End Products, Advanced; D006038:Glyoxylates; D012709:Serum Albumin; D013447:Sulfites; D011765:Pyruvaldehyde; D003847:Deoxyglucose; C016350:3-deoxyglucosone; D005947:Glucose; C025026:sodium sulfite; D000111:Acetylcysteine