Transcriptional state and chromatin structure of the murine entactin and laminin gamma1 genes.
The positions of nucleosomes in the proximal 5' regions of the coordinately regulated murine entactin/nidogen and laminin gamma1 genes have been identified in four different transcriptional states--constitutively off, basal, induced, and constitutively induced. In the entactin gene a 450 base pair (bp) region of open chromatin is present between three positioned nucleosomes and the transcriptional start site in the basal, induced, and constitutively induced states. Additionally there is a 200 bp open chromatin region at approximately -2.1 kbp that is only present in the induced and constitutively induced states. In the laminin gamma1 gene, a 650 bp region of nucleosome-free chromatin is present between nucleosomes positioned at approximately -750 and +120 in all transcriptionally active states. These results suggest that basal co-expression of these genes requires sites present in these near upstream regions. The induction to high levels appears to involve additional sites and possibly the production of new and/or the modification of existing trans-acting factors.
Chen
Y
Y
Department of Biochemistry and Molecular Biology, Finch University of Health Sciences/The Chicago Medical School, North Chicago, IL 60064, USA.
Keller
J M
JM
D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D002843:Chromatin / Q000235:genetics* / Q000648:ultrastructure; D002874:Chromosome Mapping; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D005796:Genes; D007797:Laminin / Q000096:biosynthesis / Q000235:genetics*; D008562:Membrane Glycoproteins / Q000096:biosynthesis / Q000235:genetics*; D051379:Mice; D009101:Multiple Myeloma / Q000473:pathology; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000235:genetics; D009707:Nucleosomes / Q000235:genetics / Q000648:ultrastructure; D018243:Teratocarcinoma / Q000473:pathology; D014158:Transcription, Genetic; D014212:Tretinoin / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism
D002843:Chromatin; D007797:Laminin; D008562:Membrane Glycoproteins; D009363:Neoplasm Proteins; D009707:Nucleosomes; C089141:laminin gamma 1; C029967:nidogen; D014212:Tretinoin; D003994:Bucladesine