Rapid confirmation of Clostridium perfringens by using chromogenic and fluorogenic substrates.
The use of 4-methylumbelliferyl phosphate (MUP) and ortho-nitrophenyl-beta-D-galactopyranoside (ONPG) for the identification of Clostridium perfringens was investigated. A liquid assay containing both MUP and ONPG was a highly specific alternative method for C. perfringens confirmation, reducing incubation time from 48 to only 4 h. The assay solution is easy to prepare, does not require anaerobic conditions for use, and has an extended shelf life.
Adcock
P W
PW
Microbiology Unit, Australian Water Quality Centre, SA Water Corporation, Salisbury, South Australia 5108, Australia.
Saint
C P
CP
D000135:Acid Phosphatase / Q000378:metabolism; D015373:Bacterial Typing Techniques / Q000379:methods; D002863:Chromogenic Compounds / Q000378:metabolism; D003016:Clostridium perfringens / Q000145:classification* / Q000201:enzymology* / Q000254:growth & development; D003470:Culture Media; D005456:Fluorescent Dyes / Q000378:metabolism; D006923:Hymecromone / Q000031:analogs & derivatives* / Q000378:metabolism*; D009598:Nitrophenylgalactosides / Q000378:metabolism*; D012680:Sensitivity and Specificity; D013997:Time Factors
D002863:Chromogenic Compounds; D003470:Culture Media; D005456:Fluorescent Dyes; C055012:2-nitrophenylgalactoside; D009598:Nitrophenylgalactosides; C005359:4-methylumbelliferyl phosphate; D006923:Hymecromone; D000135:Acid Phosphatase