title: Partial liquid ventilation improves lung function in ventilation-induced lung injury.
abstract: Disturbances in lung function and lung mechanics are present after ventilation with high peak inspiratory pressures (PIP) and low levels of positive end-expiratory pressure (PEEP). Therefore, the authors investigated whether partial liquid ventilation can re-establish lung function after ventilation-induced lung injury. Adult rats were exposed to high PIP without PEEP for 20 min. Thereafter, the animals were randomly divided into five groups. The first group was killed immediately after randomization and used as an untreated control. The second group received only sham treatment and ventilation, and three groups received treatment with perfluorocarbon (10 mL x kg(-1), 20 mL x kg(-1), and 20 ml x kg(-1) plus an additional 5 mL x kg(-1) after 1 h). The four groups were maintained on mechanical ventilation for a further 2-h observation period. Blood gases, lung mechanics, total protein concentration, minimal surface tension, and small/large surfactant aggregates ratio were determined. The results show that in ventilation-induced lung injury, partial liquid ventilation with different amounts of perflubron improves gas exchange and pulmonary function, when compared to a group of animals treated with standard respiratory care. These effects have been observed despite the presence of a high intra-alveolar protein concentration, especially in those groups treated with 10 and 20 mL of perflubron. The data suggest that replacement of perfluorocarbon, lost over time, is crucial to maintain the constant effects of partial liquid ventilation.
authors.affiliation: Dept of Anesthesiology, Erasmus University Rotterdam, The Netherlands.
authors.forename: G F
authors.initials: GF
authors.lastname: Vazquez de Anda
authors.forename: R A
authors.initials: RA
authors.lastname: Lachmann
authors.forename: S J
authors.initials: SJ
authors.lastname: Verbrugge
authors.forename: D
authors.initials: D
authors.lastname: Gommers
authors.forename: J J
authors.initials: JJ
authors.lastname: Haitsma
authors.forename: B
authors.initials: B
authors.lastname: Lachmann
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D005466:Fluorocarbons / Q000008:administration & dosage; D007385:Intermittent Positive-Pressure Ventilation; D021061:Liquid Ventilation; D008176:Lung Volume Measurements; D008297:Male; D011175:Positive-Pressure Respiration; D011649:Pulmonary Alveolar Proteinosis / Q000503:physiopathology*; D011659:Pulmonary Gas Exchange / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D015656:Respiratory Mechanics / Q000502:physiology; D014939:Work of Breathing / Q000502:physiology
chemical_list: D005466:Fluorocarbons