title: Isolation and genetic characterization of two G3P5A[3] canine rotavirus strains in Italy.
abstract: Two strains of canine rotavirus were isolated from pups with clinical signs of gastroenteritis. Both strains were identified by polymerase chain reaction (PCR) as G3P5A[3], although restriction endonuclease analysis of the PCR amplicons revealed a genetic difference between the two isolates in the VP7 gene. The isolation in Italy of canine rotaviruses displaying the same VP7 and VP4 specificities as in the USA and in Japan, suggests that the G3 and P5A[3] types are highly conserved among canine rotavirus strains.
authors.affiliation: Faculty of Veterinary Medicine, Department of Animal Health and Wellbeing, University of Bari, Bari, Italy. v.martella@veterinaria.uniba.it
authors.forename: V
authors.initials: V
authors.lastname: Martella
authors.forename: A
authors.initials: A
authors.lastname: Pratelli
authors.forename: G
authors.initials: G
authors.lastname: Elia
authors.forename: N
authors.initials: N
authors.lastname: Decaro
authors.forename: M
authors.initials: M
authors.lastname: Tempesta
authors.forename: C
authors.initials: C
authors.lastname: Buonavoglia
mesh_terms: D000818:Animals; D000956:Antigens, Viral; D002213:Capsid / Q000235:genetics; D036022:Capsid Proteins; D004283:Dog Diseases / Q000821:virology*; D004285:Dogs; D005759:Gastroenteritis / Q000662:veterinary* / Q000821:virology; D007558:Italy; D008854:Microscopy, Electron; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D012401:Rotavirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000662:veterinary* / Q000821:virology
chemical_list: D000956:Antigens, Viral; D036022:Capsid Proteins; C060339:VP4 protein, Rotavirus; C050779:VP7 protein, Rotavirus