[Study of the DNA primary structure effect on induction of mutations by alkylating agents].
Analysis and comparison of mutation spectra is one of the major tasks of molecular biology, since mutation spectra often reveal important properties of various mutagens and proteins involved in the repair/replication systems. Mutability is known to vary significantly along the nucleotide sequence. Mutations are abundant at certain positions (mutation hotspots). In this work, we applied regression analysis based on the basic logic patterns to understand the role of the nucleotide sequence context in mutation induction. The spectra of mutations induced by various alkylating agents were studied. The nucleotide bases at positions -2, -1, +1 and +2 were shown to have the most significant effect in G:C-->A:T replacements.
Rogozin
I B
IB
Institute of Cytology and Genetics, Russian Academy of Science, Novosibirsk, 630090 Russia. rogozin@bionet.nsc.ru
Berikov
V B
VB
Vasiunina
E A
EA
Sinitsina
O I
OI
D000477:Alkylating Agents / Q000633:toxicity*; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D009153:Mutagens / Q000633:toxicity*; D009154:Mutation / Q000187:drug effects; D012044:Regression Analysis
D000477:Alkylating Agents; D004269:DNA, Bacterial; D009153:Mutagens
Issledovanie vliianiia osobennosteĭ pervichnoĭ struktury DNK na vozniknovenie mutatsiĭ, indutsirovannykh alkiliruuiushchimi agentami.