Biochemically functionalized silica nanoparticles.
In this report, we demonstrate the biochemical modification of silica based nanoparticles. Both pure and dye-doped silica nanoparticles were prepared, and their surfaces were modified with enzymes and biocompatible chemical reagents that allow them to function as biosensors and biomarkers. The nanoparticles produced in this work are uniform in size with a 1.6% relative standard deviation. They have a pure silica surface and can thus be modified easily with many biomolecules for added biochemical functionality. Specifically, we have modified the nanoparticle surfaces with enzyme molecules (glutamate dehydrogenase (GDH) and lactate dehydrogenase (LDH)) and a biocompatible reagent for cell membrane staining. Experimental results show that the silica nanoparticles are a good biocompatible solid support for enzyme immobilization. The immobilized enzyme molecules on the nanoparticle surface have shown excellent enzymatic activity in their respective enzymatic reactions. The nanoparticle surface biochemical functionalization demonstrates the feasibility of using nanoparticles for biosensing and biomarking applications.
Qhobosheane
M
M
Center for Research at the Interface of Bio/nano, Department of Chemistry and the McKnight Brain Institute, University of Florida, Gainesville 32611, USA.
Santra
S
S
Zhang
P
P
Tan
W
W
D015374:Biosensing Techniques; D002214:Capsules; D004396:Coloring Agents; D005969:Glutamate Dehydrogenase; D018698:Glutamic Acid / Q000032:analysis*; D007770:L-Lactate Dehydrogenase; D019344:Lactic Acid / Q000032:analysis*; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D008863:Microspheres; D012822:Silicon Dioxide
D002214:Capsules; D004396:Coloring Agents; D019344:Lactic Acid; D018698:Glutamic Acid; D012822:Silicon Dioxide; D007770:L-Lactate Dehydrogenase; D005969:Glutamate Dehydrogenase