title: Effects of Buddleja globosa leaf and its constituents relevant to wound healing.
abstract: An aqueous extract of Buddleja globosa leaves, used traditionally in Chile for wound healing, was tested for the ability to stimulate growth of fibroblasts in vitro and for antioxidant activity in the same fibroblast cell system challenged with hydrogen peroxide. Low concentrations of the extract gave an increase in fibroblast growth which was not statistically significant but cytotoxicity was observed at concentrations greater than 50 microg/ml. The extract showed strong antioxidant effect and fractionation led to the isolation of three flavonoids and two caffeic acid derivatives, each of which was shown to contribute to the antioxidant effect at concentrations below 10 microg/ml. These activities would accelerate the healing of wounds.
authors.affiliation: Pharmacognosy Research Laboratories, Department of Pharmacy, King's College London, 150 Stamford Street, London SE1 8WA, UK.
authors.forename: A Y
authors.initials: AY
authors.lastname: Mensah
authors.forename: J
authors.initials: J
authors.lastname: Sampson
authors.forename: P J
authors.initials: PJ
authors.lastname: Houghton
authors.forename: P J
authors.initials: PJ
authors.lastname: Hylands
authors.forename: J
authors.initials: J
authors.lastname: Westbrook
authors.forename: M
authors.initials: M
authors.lastname: Dunn
authors.forename: M A
authors.initials: MA
authors.lastname: Hughes
authors.forename: G W
authors.initials: GW
authors.lastname: Cherry
mesh_terms: D000975:Antioxidants / Q000302:isolation & purification / Q000627:therapeutic use*; D002478:Cells, Cultured; D002677:Chile; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000187:drug effects*; D006801:Humans; D006861:Hydrogen Peroxide / Q000037:antagonists & inhibitors; D008517:Phytotherapy; D010936:Plant Extracts / Q000302:isolation & purification / Q000627:therapeutic use*; D018515:Plant Leaves; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D006861:Hydrogen Peroxide