Quantitation of the factor VII- and single-chain plasminogen activator-activating protease in plasmas of healthy subjects.
Plasma samples of 189 healthy subjects were investigated for antigen levels of the recently reported factor VII- and single-chain plasminogen activator-activating protease (FSAP) and the corresponding pro-urokinase activating potencies. While the age of donors had no significant effect on the investigated parameters, female plasmas revealed a trend to higher antigen contents and activity levels. Surprisingly, as much as 9% of all samples contained significantly reduced single-chain urinary plasminogen activator activating potential, whereas antigen concentrations were normal. Additionally, 1% of the plasmas was found to decrease in both FSAP antigen and activity contents. FSAP of three subjects displaying reduced activities throughout a follow-up period of 6 months were purified from plasmas and were characterized. As compared with pool plasma derived FSAP, investigation of the individual preparations confirmed their reduced potency to activate pro-urokinase. However, factor VII activation was not affected. It is speculated that the FSAP binding site for single-chain plasminogen activators is affected, potentially by as yet unknown polymorphism(s) or mutation(s).
Römisch
J
J
Aventis Behring GmbH, Research, Marburg, Germany. Juergen.Roemisch@aventis.com
Feussner
A
A
Stöhr
H A
HA
D000328:Adult; D000367:Age Factors; D000368:Aged; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011994:Recombinant Proteins / Q000187:drug effects / Q000378:metabolism; D012697:Serine Endopeptidases / Q000097:blood* / Q000737:chemistry / Q000494:pharmacology*; D012737:Sex Factors; D013997:Time Factors; D014568:Urokinase-Type Plasminogen Activator / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*
D011994:Recombinant Proteins; C433550:HABP2 protein, human; D012697:Serine Endopeptidases; D014568:Urokinase-Type Plasminogen Activator; C067551:saruplase