Cryptogein affects expression of alpha3, alpha6 and beta1 20S proteasome subunits encoding genes in tobacco.
Twelve alpha and beta 20S proteasome subunits cDNAs showing 70-82% identity with the corresponding genes in Arabidopsis or rice, and features of eukaryotic proteasome subunits were cloned in tobacco. Only beta1-tcI 7, alpha3 and alpha6, 20S proteasome subunits encoding genes were up-regulated by cryptogein, a proteinaceous elicitor of plant defence reactions. These results led to the hypothesis that the activation of beta1-tcI 7, alpha3 and alpha6 could induce a specific proteolysis involved in the hypersensitive response and systemic acquired resistance monitored by cryptogein.
Dahan
J
J
UMR INRA-Université de Bourgogne, Phytopharmacie et Biochimie des Interactions Cellulaires, INRA BP 86510, 21065 Dijon cedex, France.
Etienne
P
P
Petitot
A S
AS
Houot
V
V
Blein
J P
JP
Suty
L
L
D020418:Algal Proteins / Q000502:physiology*; D000595:Amino Acid Sequence; D003546:Cysteine Endopeptidases / Q000378:metabolism*; D005656:Fungal Proteins; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000378:metabolism*; D010935:Plant Diseases; D030821:Plants, Genetically Modified; D046988:Proteasome Endopeptidase Complex; D014026:Nicotiana / Q000201:enzymology* / Q000235:genetics
D020418:Algal Proteins; D005656:Fungal Proteins; D009097:Multienzyme Complexes; C061032:cryptogein protein, Phytophthora cryptogea; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex