In vivo recombination as a tool to generate molecular diversity in phage antibody libraries.
The creation of diversity in populations of polypeptides has become an important tool in the derivation of polypeptides with useful characteristics. This requires efficient methods to create diversity coupled with methods to select polypeptides with desired properties. In this review we describe the use of in vivo recombination as a powerful way to generate diversity. The novel principles for the recombination process and several applications of this process for the creation of phage antibody libraries are described. The advantage and disadvantages are discussed and possible future exploitation presented.
Sblattero
D
D
Dipartimento di Biologia, Universita' di Trieste, Italy.
Lou
J
J
Marzari
R
R
Bradbury
A
A
D000818:Animals; D000906:Antibodies / Q000235:genetics*; D001709:Biotechnology; D014644:Genetic Variation; D005822:Genetic Vectors; D023281:Genomics; D006801:Humans; D019426:Integrases; D019151:Peptide Library; D015202:Protein Engineering; D011995:Recombination, Genetic; D014764:Viral Proteins
D000906:Antibodies; D019151:Peptide Library; D014764:Viral Proteins; C045073:Cre recombinase; D019426:Integrases