Analysis of Ti-6Al-4V implants placed with fibroblast growth factor 1 in  rat tibiae.
Titanium-aluminum-vanadium (Ti-6Al-4V) implants were placed in the tibiae of 32 rats (male Sprague-Dawley, 350 g) to examine healing and bone response. Half of the implants were treated with fibroblast growth factor 1 (FGF-1) delivered in an activated fibrinogen matrix. Animals were injected with a radiopharmaceutical imaging agent, technetium-99m-methylene diphosphonate (Tc-99m-MDP), which concentrates in bone, especially in areas of higher osteoblastic activity. Binding of Tc-99m-MDP to the implant was detected in vivo by Anger gamma camera imaging. Fourteen days after implant surgery, specimens were recovered and prepared for histomorphometric analysis. Histologic examination revealed that samples treated with FGF-1 demonstrated significantly greater amounts of bone-to-implant contact (P < .05) compared to controls. Also, FGF-1-treated samples showed significantly greater amounts of bone (percent volume) adjacent to implants (P < .005). These findings were supported by analyses of the non-invasive Tc-99m-MDP images, which demonstrated significantly greater uptake of Tc-99m-MDP adjacent to FGF-1-treated implants (P < .05). Results of the experiments supported the hypothesis that FGF-1 could increase bone production around implants in a rat model.
McCracken
M
M
Department of Prosthodontics and Biomaterials, University of Alabama School of Dentistry, Birmingham 35294-0007, USA. mikemc@uab.edu
Lemons
J E
JE
Zinn
K
K
D000497:Alloys / Q000737:chemistry; D000818:Animals; D001863:Bone Screws; D004337:Drug Carriers; D015718:Fibrin Tissue Adhesive; D016220:Fibroblast Growth Factor 1; D016222:Fibroblast Growth Factor 2 / Q000627:therapeutic use*; D005500:Follow-Up Studies; D015902:Gamma Cameras; D007091:Image Processing, Computer-Assisted; D008297:Male; D023421:Models, Animal; D015999:Multivariate Analysis; D016348:Osseointegration; D010006:Osteoblasts / Q000187:drug effects; D010012:Osteogenesis / Q000187:drug effects; D010027:Osteotomy; D011877:Radionuclide Imaging; D019275:Radiopharmaceuticals; D051381:Rats; D017207:Rats, Sprague-Dawley; D013223:Statistics as Topic; D013499:Surface Properties; D013669:Technetium Tc 99m Medronate; D013977:Tibia / Q000000981:diagnostic imaging / Q000187:drug effects / Q000473:pathology / Q000601:surgery*; D014025:Titanium / Q000737:chemistry; D014915:Whole-Body Counting; D014945:Wound Healing / Q000187:drug effects
D000497:Alloys; D004337:Drug Carriers; D015718:Fibrin Tissue Adhesive; D019275:Radiopharmaceuticals; D016222:Fibroblast Growth Factor 2; D016220:Fibroblast Growth Factor 1; C031462:titanium alloy (TiAl6V4); D014025:Titanium; D013669:Technetium Tc 99m Medronate