A highly specific one-step PCR - assay for the rapid discrimination of enteropathogenic Yersinia enterocolitica from pathogenic Yersinia pseudotuberculosis and Yersinia pestis.
Based on differences within the yopT-coding region of Yersinia. enterocolitica, Y pseudotuberculosis and Y pestis, a rapid and sensitive one-step polymerase chain reaction assay with high specificity for pathogenic Y enterocolitica was developed. By this method pathogenic isolates of Y enterocolitica can be easily identified and discriminated from other members of this genus. The entire coding sequence of the yopT effector gene of Y. pseudotuberculosis Y36 was determined.
Arnold
T
T
Institute of Animal Hygiene and Public Veterinary Health, Leipzig, Germany.
Hensel
A
A
Hagen
R
R
Aleksic
S
S
Neubauer
H
H
Scholz
H C
HC
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D003546:Cysteine Endopeptidases; D003603:Cytotoxins; D005243:Feces / Q000382:microbiology; D006168:Guinea Pigs; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D013997:Time Factors; D015009:Yersinia Infections / Q000382:microbiology*; D015008:Yersinia enterocolitica / Q000145:classification* / Q000235:genetics / Q000472:pathogenicity; D015010:Yersinia pestis / Q000145:classification* / Q000235:genetics / Q000472:pathogenicity; D015011:Yersinia pseudotuberculosis / Q000145:classification* / Q000235:genetics / Q000472:pathogenicity
D001426:Bacterial Proteins; D003603:Cytotoxins; C116117:YopT protein, Yersinia; D003546:Cysteine Endopeptidases