Cooking oil fume-induced cytokine expression and oxidative stress in human lung epithelial cells.
Epidemiological studies have shown an association between exposure to indoor air pollution from Chinese-style cooking and risk of lung cancer among Chinese females. Several toxic substances have been identified in cooking oil fumes (COF) collected from heated rapeseed oil. In this study, we examined the biological effects of COF on CL3 human lung epithelial cells. Exposure to 200 microg/ml COF significantly reduced cell growth within 4 days. In addition, we examined the effect of COF on TGFbeta1, TGFbeta2, IL-6, IL-8, and IFN-gamma gene expressions with the RT-PCR method. We found that TGFbeta1 mRNA levels increased after exposure to 200 microg/ml COF for 24 h. Similarly, exposure to 10 microM benzo[a]pyrene or 100 nM 12-O-tetradecanoylphorbol-13-acetate increased TGFbeta1 mRNA levels at 24 h. The mRNA levels of TGFbeta2, IL-6, IL-8, and IFN-gamma did not increase after treatment with COF, benzo[a]pyrene, or 12-O-tetradecanoylphorbol-13-acetate. COF-induced TGFbeta1 production was confirmed by quantification of TGFbeta1 in conditioned medium with enzyme-linked immunosorbent assay. Exposure to 200 microg/ml COF significantly increased TGFbeta1 secretion in a time-dependent and dose-dependent manner. It has been demonstrated that reactive oxygen intermediates induce TGFbeta1 gene expression. When CL3 cells were exposed to 200 microg/ml COF for 15 min, there was an increase in intracellular peroxide formation with the dichlorofluorescein method. Furthermore, treatment with 200 microg/ml COF for 12 h also significantly induced lipid peroxidation in CL3 cells. Our results show that exposure to COF inhibits cell growth, increases TGFbeta1 secretion, and induces oxidative stress in CL3 lung epithelial cells. This suggests that TGFbeta1 and oxidative stress play a role in the biological effects of COF on lung epithelial cells.
Tung
Y H
YH
Institute of Toxicology, Chung-Shan Medical and Dental College, Taichung 40203, Taiwan, Republic of China.
Ko
J L
JL
Liang
Y F
YF
Yin
L
L
Pu
Y
Y
Lin
P
P
D016902:Air Pollution, Indoor / Q000009:adverse effects*; D018929:Cell Culture Techniques; D002455:Cell Division; D002681:China; D003296:Cooking; D016207:Cytokines / Q000096:biosynthesis*; D017931:DNA Primers; D004847:Epithelial Cells / Q000502:physiology*; D005786:Gene Expression Regulation; D006801:Humans; D008168:Lung / Q000166:cytology* / Q000473:pathology; D018384:Oxidative Stress; D010938:Plant Oils; D020133:Reverse Transcriptase Polymerase Chain Reaction
D016207:Cytokines; D017931:DNA Primers; D010938:Plant Oils