[Levels and molecular heterogeneity of serotonin transporter protein in platelets of patients with different mental diseases: a comparative analysis with the use of monoclonal and polyclonal antibodies].
Polyclonal (PAb) and monoclonal (MAb) antibodies to CT2-epitope of the C-terminal fragment of serotonin transporter (SERT) protein were used to study the levels and molecular heterogeneity of platelet SERT in healthy donors and patients with affective (AD) and somatoform (SD) disorders, schizoaffective disorder (SAD) and schizophrenia. SERT was found to exist as high molecular wight (HMW) and low molecular weight (LMW) forms separated after electrophoresis. The levels of HMW and LMW forms of SERT were significantly, decreased in mentally ill patients as compared to healthy individuals. Unlike PAb, horse radish peroxidase (HRP)-conjugated MAbs were more sensitive and specific to SERT and could detect the LMW form of SERT as a duplet protein form with MW about 40 and 43 kDa. The MAb to CT2 C-terminal fragment of SERT conjugated with HRP is considered to be a new valuable tool for further investigation of SERT expression, properties, and posttranslation modification in the controls and in patients with different psychopathology.
Brusov
O S
OS
Faktor
M I
MI
Zlobina
G P
GP
Bologov
P V
PV
Kaleda
V G
VG
Oleĭchik
I V
IV
Korenev
A N
AN
Piatnitskiĭ
A N
AN
Dupin
A M
AM
Katasonov
A B
AB
Morozova
M A
MA
Beniashvili
A G
AG
Lozier
R Kh
RKh
Pavlova
E V
EV
Segal
O L
OL
Massino
Iu S
IuS
Dmitriev
A D
AD
D000328:Adult; D000818:Animals; D000911:Antibodies, Monoclonal; D001792:Blood Platelets / Q000378:metabolism*; D002352:Carrier Proteins / Q000097:blood* / Q000235:genetics / Q000378:metabolism; D004586:Electrophoresis; D000939:Epitopes; D005260:Female; D018740:Genetic Heterogeneity; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008562:Membrane Glycoproteins / Q000097:blood* / Q000235:genetics / Q000378:metabolism; D026901:Membrane Transport Proteins; D001523:Mental Disorders / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008875:Middle Aged; D008970:Molecular Weight; D019964:Mood Disorders / Q000097:blood / Q000235:genetics / Q000378:metabolism; D009419:Nerve Tissue Proteins; D021381:Protein Transport; D012559:Schizophrenia / Q000097:blood / Q000235:genetics / Q000378:metabolism; D012680:Sensitivity and Specificity; D012701:Serotonin / Q000378:metabolism*; D050486:Serotonin Plasma Membrane Transport Proteins; D013001:Somatoform Disorders / Q000097:blood / Q000235:genetics / Q000378:metabolism
D000911:Antibodies, Monoclonal; D002352:Carrier Proteins; D000939:Epitopes; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D009419:Nerve Tissue Proteins; C493230:SLC6A4 protein, human; D050486:Serotonin Plasma Membrane Transport Proteins; C493231:Slc6a4 protein, mouse; D012701:Serotonin
Soderzhanie i molekuliarnaia geterogennost' belka--perenoschika serotonina v trombotsitakh bol'nykh s razlichnymi psikhicheskimi zabolevaniiami: sravnitel'nyĭ analiz s ispol'zovaniem monoklonal'nykh i poliklonal'nykh antitel.