title: Demonstration of intravital microfissures in undecalcified plastic-embedded temporal bones with the prestaining technique.
abstract: Microfissures in the human otic capsule have been observed since the start of the century, but it was Otto Mayer, in 1930, who first realized that some of them were of intravital origin and not just processing artifacts. Since then, a small number of publications, based on decalcified temporal bones, have mostly confirmed his findings. With the introduction by Frost in the late 1950s of the undecalcified bone technique and the bulk staining technique for peripheral bones, a method was developed and refined for identifying even very small intravital microfissures (microdamage). Bulk staining of undecalcified otic capsules has not yet been used to verify the findings from the previous decalcified specimens. The present report presents our experience with the pertinent techniques, and suggests modifications and shortcuts pertinent to temporal bone research. Both large and tiny microfissures of intravital genesis are demonstrable within bulk-stained undecalcified human otic capsules. The importance of microfissures in the petrous bone in the causation of otosclerosis and perilymphatic leakage has long been discussed, and the present techniques may advance our understanding of these pathological conditions
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Rigshospitalet, Copenhagen, Denmark.
authors.forename: T
authors.initials: T
authors.lastname: Frisch
authors.forename: M S
authors.initials: MS
authors.lastname: Sørensen
authors.forename: P
authors.initials: P
authors.lastname: Bretlau
mesh_terms: D000328:Adult; D016477:Artifacts; D006652:Histological Techniques; D006801:Humans; D020366:Methylmethacrylate; D008856:Microscopy, Fluorescence; D016613:Plastic Embedding; D012394:Rosaniline Dyes; D013194:Staining and Labeling / Q000379:methods; D013701:Temporal Bone / Q000033:anatomy & histology*
chemical_list: D012394:Rosaniline Dyes; D020366:Methylmethacrylate