title: Permeability of human HT-29/B6 colonic epithelium as a function of apoptosis.
abstract: 1. The barrier function of colonic epithelia is challenged by apoptotic loss of enterocytes. In monolayers of human colonic HT-29/B6 cells, apoptosis induced by camptothecin was assessed by poly-(ADP-ribose)-polymerase (PARP) cleavage, histone ELISA and DNA-specific fluorochrome staining (with 4',6'-diamidino-2'-phenylindoladihydrochloride (DAPI)). Epithelial barrier function was studied in Ussing chambers by measuring transepithelial conductivity and unidirectional tracer fluxes. The ion permeability associated with single cell apoptoses was investigated with the conductance scanning technique. 2. The spontaneous rate of apoptotic cells was 3.5 +/- 0.3 % with an overall epithelial conductivity of 3.2 +/- 0.1 mS cm(-2). Camptothecin induced a time- and dose-dependent increase of apoptosis and permeability. With 20 microg ml(-1) of camptothecin for 48 h, apoptosis increased 4.1-fold to 14.3 +/- 1.5 % and the conductivity doubled to 6.4 +/- 1.0 mS cm(-2). 3. While 3H-mannitol flux increased 3.8-fold and 3H-lactulose flux increased 2.6-fold, the flux of 3H-polyethylene glycol 4000 remained unchanged. Hence, the higher permeability was limited to molecules < 4000 Da. 4. The local epithelial conductivity was higher at the sites of apoptosis than in non-apoptotic areas. With camptothecin the leaks associated with apoptosis became more numerous and more conductive, while in non-apoptotic areas the conductivity remained at control level. Hence, the camptothecin-induced increase in epithelial conductivity reflected the opening of apoptotic leaks and thus the results described, for the first time, epithelial permeability as a function of apoptosis only. 5. The conductivity of apoptotic leaks contributed 5.5 % to the epithelial conductivity of controls and 60 % to the conductivity of monolayers treated with 20 microg ml(-1) of camptothecin. Thus apoptosis increased the contribution of paracellular pathways to the overall epithelial permeability. Under control conditions the paracellular conductivity (G(para)) was smaller than the transcellular (G(trans)), but with 12 % apoptosis, G(para) exceeded G(trans). By definition, the epithelium became 'leaky'.
authors.affiliation: Department of Gastroenterology, Universitätsklinikum Benjamin Franklin, Freie Universität Berlin, 12200 Berlin, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Bojarski
authors.forename: A H
authors.initials: AH
authors.lastname: Gitter
authors.forename: K
authors.initials: K
authors.lastname: Bendfeldt
authors.forename: J
authors.initials: J
authors.lastname: Mankertz
authors.forename: H
authors.initials: H
authors.lastname: Schmitz
authors.forename: S
authors.initials: S
authors.lastname: Wagner
authors.forename: M
authors.initials: M
authors.lastname: Fromm
authors.forename: J D
authors.initials: JD
authors.lastname: Schulzke
mesh_terms: D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D002166:Camptothecin / Q000494:pharmacology; D002463:Cell Membrane Permeability / Q000187:drug effects / Q000502:physiology; D003106:Colon / Q000166:cytology* / Q000378:metabolism*; D004234:Diuretics, Osmotic / Q000493:pharmacokinetics; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay; D005456:Fluorescent Dyes; D005765:Gastrointestinal Agents / Q000493:pharmacokinetics; D019073:HT29 Cells; D006657:Histones / Q000032:analysis; D006801:Humans; D007211:Indoles; D007413:Intestinal Mucosa / Q000166:cytology* / Q000378:metabolism*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D007792:Lactulose / Q000493:pharmacokinetics; D008353:Mannitol / Q000493:pharmacokinetics; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D011092:Polyethylene Glycols / Q000493:pharmacokinetics; D012997:Solvents / Q000493:pharmacokinetics; D013194:Staining and Labeling; D019311:Staurosporine / Q000494:pharmacology; D014316:Tritium
chemical_list: D004234:Diuretics, Osmotic; D004791:Enzyme Inhibitors; D005456:Fluorescent Dyes; D005765:Gastrointestinal Agents; D006657:Histones; D007211:Indoles; D012997:Solvents; D014316:Tritium; D008353:Mannitol; D011092:Polyethylene Glycols; D007792:Lactulose; C007293:DAPI; D007770:L-Lactate Dehydrogenase; D011065:Poly(ADP-ribose) Polymerases; D019311:Staurosporine; D002166:Camptothecin