title: The difference in E-cadherin expression between nonvascularized and vascularized nerve grafts: study in the rat sciatic nerve model.
abstract: BACKGROUND
  We investigated the expression of E-cadherin during nerve regeneration after nonvascularized and vascularized nerve grafts.
  
  
  METHODS
  We used the rat sciatic nerve model. E-cadherin expression was detected by Western blot analysis and immunofluorescent staining with anti E-cadherin monoclonal antibody. The level of E-cadherin expression was calculated as the amount relative to that of E-cadherin expression of normal control nerve. Furthermore, repair of the neural tissue structure was examined by toluidine blue staining.
  
  
  RESULTS
  In both cases, the level of E-cadherin expression decreased at first, and then gradually increased. The maximum level was 1.61 +/- 0.066-fold in the nonvascularized nerve graft and 2.254 +/- 0.071-fold in the vascularized nerve graft. From the 1st to the 16th postoperative weeks, the level of E-cadherin expression in the vascularized nerve graft was significantly higher than that in the nonvascularized nerve graft. In the immunofluorescent staining, E-cadherin expression was almost negative or decreased immediately after the operation, but the degree of expression was gradually increased in Schwann cells. The degree of E-cadherin expression in the vascularized nerve graft was greater than that in the nonvascularized nerve graft. In toluidine blue staining, the velocity of tissue repair was more rapid in the vascularized nerve graft than in the nonvascularized graft.
  
  
  CONCLUSIONS
  These results demonstrate that the E-cadherin expression of grafted nerve was increased during the nerve regeneration, and the expression was mainly observed in Schwann cells. Because the level of E-cadherin expression was significantly higher in the vascularized nerve graft than in the nonvascularized nerve graft, the level of E-cadherin expression may affect the rapidity of nerve regeneration.
authors.affiliation: Division of Plastic Surgery, Nara Medical University, 840 Shijo-cho, Kashihara, Nara, 634-8521, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Tada
authors.forename: M
authors.initials: M
authors.lastname: Hatoko
authors.forename: A
authors.initials: A
authors.lastname: Tanaka
authors.forename: M
authors.initials: M
authors.lastname: Kuwahara
authors.forename: K
authors.initials: K
authors.lastname: Mashiba
authors.forename: S
authors.initials: S
authors.lastname: Yurugi
mesh_terms: D000818:Animals; D015153:Blotting, Western; D015820:Cadherins / Q000032:analysis / Q000096:biosynthesis*; D004396:Coloring Agents; D005455:Fluorescent Antibody Technique; D008297:Male; D023421:Models, Animal; D009416:Nerve Regeneration / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D012583:Schwann Cells / Q000737:chemistry / Q000378:metabolism; D012584:Sciatic Nerve / Q000098:blood supply* / Q000378:metabolism / Q000637:transplantation*; D013194:Staining and Labeling; D014048:Tolonium Chloride
chemical_list: D015820:Cadherins; D004396:Coloring Agents; D014048:Tolonium Chloride