Preserved endothelial integrity and nitric oxide synthase in saphenous vein grafts harvested by a 'no-touch' technique.
BACKGROUND
The saphenous vein is the most commonly used conduit for coronary artery bypass surgery, but 1-year occlusion rates as high as 30 per cent have been reported. In conventional surgery, considerable damage to the vein occurs during harvesting. The aim of this study was to compare endothelial integrity and nitric oxide synthase (NOS) in saphenous veins harvested by a novel 'no-touch' technique and veins harvested conventionally.


METHODS
Immunohistochemistry was used to study endothelial integrity, and a combination of histochemistry and autoradiography was employed to identify NOS in human saphenous veins harvested by conventional and no-touch techniques.


RESULTS
The endothelial lining of conventional grafts was reduced compared with that of no-touch grafts (52 versus 73 per cent; P = 0.04). This was associated with a concomitant reduction of NOS availability; NOS was also present in the adventitial vasa vasorum of no-touch vessels.


CONCLUSIONS
Some of the sites with potential for nitric oxide release in vivo are removed during conventional saphenous vein harvesting. These sites were preserved after no-touch harvesting, suggesting the potential to improve coronary artery bypass graft patency.
Tsui
J C
JC
Department of Surgery, Royal Free and University College Medical School, London, UK.
Souza
D S
DS
Filbey
D
D
Bomfim
V
V
Dashwood
M R
MR
D000368:Aged; D000369:Aged, 80 and over; D001345:Autoradiography; D003094:Collagen / Q000378:metabolism; D001026:Coronary Artery Bypass / Q000379:methods; D004730:Endothelium, Vascular / Q000201:enzymology* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D009252:NADPH Dehydrogenase / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000378:metabolism*; D012501:Saphenous Vein / Q000201:enzymology / Q000473:pathology / Q000637:transplantation*; D013048:Specimen Handling / Q000009:adverse effects
D003094:Collagen; D019001:Nitric Oxide Synthase; D009252:NADPH Dehydrogenase