[Relationship between serum matrix metalloproteinase-2 and metastasis and recurrence following radical hepatic resection in hepatocellular carcinoma].
OBJECTIVE
To study the relationship between serum matrix metalloproteinase-2 (MMP-2) and metastasis and recurrence following radical hepatic resection in hepatocellular carcinoma (HCC).


METHODS
Cox's proportional hazard model was used to determine the significant clinical factor of prognosis. Meanwhile, enzyme linked immunoadsordent assay (ELISA) and northern blot analysis were adopted to detect serum and tumor tissue levels of MMP-2 respectively in HCC patients.


RESULTS
Cox's proportional hazard model showed that tumor size and tumor number were significant prognostic factors of HCC patients following hepatic resection (P=0.022 and P=0.040). According to this result, we divided HCC patients into high-tendency to metastasis recurrence and low-tendency to metastasis recurrence groups. Serum levels of MMP-2 in high-tendency to metastasis recurrence following radical hepatic resection group were significantly higher than those in low-tendency group (26.39ng/m+/-2.64ng/ml vs 24.86ng+/-1.95ng/ml, P<0.05). Serum levels of MMP-2 in HCC patients performed non-radical hepatic resection group were also significantly higher than those in HCC patients performed radical hepatic resection group (29.43ng+/-3.12 ng/ml vs 25.72ng+/-2.45ng/ml, P<0.01). Northern blot analysis revealed that the changes of MMP-2 level in the serum were consistent with those of its mRNA level in the tumor tissue of HCC patients.


CONCLUSIONS
Serum MMP-2 level is an indicator for metastatic recurrence following radical hepatic resection in HCC. The high levels of MMP-2 in the serum are due to high MMP-2 mRNA expression in the tumor tissue of HCC patients.
Niu
Q
Q
Liver Cancer Institute, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
Tang
Z
Z
Ma
Z
Z
Qin
L
L
Bao
W
W
Zhang
L
L
D006528:Carcinoma, Hepatocellular / Q000201:enzymology / Q000473:pathology / Q000601:surgery*; D006498:Hepatectomy; D006801:Humans; D008113:Liver Neoplasms / Q000201:enzymology / Q000473:pathology / Q000601:surgery*; D020778:Matrix Metalloproteinase 2 / Q000097:blood*; D009362:Neoplasm Metastasis; D009364:Neoplasm Recurrence, Local; D016016:Proportional Hazards Models
D020778:Matrix Metalloproteinase 2