Characterization of a Saccharomyces cerevisiae mutant with oversecretion phenotype.
An oversecreting mutant of Saccharomyces cerevisiae was obtained from about 400 meiotic segregants derived from thediploid cells made by crossing the HBsAg-induced mutant NI-C with the wild-type strain Sey6211. When transformed with a plasmid containing mouse alpha-amylase cDNA, the mutant (NI-C-D4) exhibited an increased capacity (up to 13-fold) for the secretion of mouse alpha-amylase, higher than the parental strains and other standard wild-type strains. It was also shown that alpha-amylase secreted by the oversecreting mutant had a higher activity and contained more of the non-glycosylated form than the glycosylated form. This isolated oversecreting, low-glycosylation mutant may prove to be a potential S. cerevisiae host for the production of foreign proteins. Further genetic analysis suggested that the mutation responsible for the mutant's oversecretion was partially dominant and that both the oversecretion and low-glycosylation phenotypes were governed by a single chromosome mutation. These pleiotrophic phenotypes may be attributed to a defect in the synthesis of an ER-resident chaperone.
Wang
B D
BD
Institute of Molecular Biology, Academia Sinica, Nankang, Taipei, Taiwan. bdwang@gate.sinica.edu.tw
Chen
D C
DC
Kuo
T T
TT
D010450:Endopeptidases / Q000378:metabolism; D005800:Genes, Fungal; D006031:Glycosylation; D015151:Immunoblotting; D009154:Mutation; D010641:Phenotype; D011994:Recombinant Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development; D014170:Transformation, Genetic; D000516:alpha-Amylases / Q000378:metabolism*
D011994:Recombinant Proteins; D000516:alpha-Amylases; D010450:Endopeptidases