The grp78 promoter of Neurospora crassa: constitutive, stress and differentiation-dependent protein-binding patterns.
In order to characterize the transcriptional regulation of the grp78 (glucose-regulated protein 78 kDa) gene of Neurospora crassa, a 1.65-kb genomic fragment upstream of the protein-coding region was sequenced and analysed. A single transcription start point was mapped 160 nt upstream of the first codon. Several distinct protein-DNA binding sites were identified in the promoter region by non-radioactive scanning electrophoretic mobility shift analysis during growth, stress treatment and differentiation of conidia. A protein DNA binding complex induced by tunicamycin was linked to a promoter motif similar to the unfolded protein response element consensus of yeast. Another binding complex in differentiating aerial hyphae was found, which differs from the known cis-elements involved in conidiation-dependent gene expression.
Monnerjahn
C
C
Institute for Cell Biology, Biochemistry and Biotechnology, University of Bremen, Germany.
Techel
D
D
Meyer
U
U
Rensing
L
L
D001483:Base Sequence; D001665:Binding Sites; D004271:DNA, Fungal / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics*; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009492:Neurospora crassa / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D014176:Protein Biosynthesis
D004271:DNA, Fungal; D005656:Fungal Proteins; D018840:HSP70 Heat-Shock Proteins; C060153:KAR2 protein, yeast