The PlA1/A2 polymorphism of platelet glycoprotein IIIa is not associated with deep venous thrombosis.
BACKGROUND
Platelet glycoprotein (GP) IIb/IIIa, a fibrinogen and von Willebrand factor binding membrane receptor, has an important role in platelet aggregation. A common leucine33-proline polymorphism (PlA1/A2) of the gene encoding the GP IIIa subunit is associated with platelet reactivity and has been proposed as a risk factor for atherothrombotic disease. The aim of this study was to investigate the role of this polymorphism for deep venous thrombosis (DVT).


METHODS
We performed a case-control study including 206 patients with documented DVT and a sex- and age-matched group of 310 control subjects. GP IIIa genotypes were determined by restriction fragment analysis of amplimers containing the polymorphic site.


RESULTS
A1/A1, A1/A2 and A2/A2 genotypes were found in 67.0, 31.6 and 1.5 percent of patients and 72.3, 25.8 and 1.9 percent of controls (p=0.35), PlA2 allele frequencies were 0.17 in patients and 0.15 in controls (p=0.92). Odds ratio of the PlA2 allele for DVT was 1.21 (95 percent CI 0.85-1.71, p=0.29) and remained insignificant after adjustment for factor V Leiden and prothrombin 20210A genotypes (1.22, 95 percent CI 0.86-1.75, p=0.27).


CONCLUSIONS
Our data suggest that the PlA1/A2 polymorphism of GP IIIa is not associated with DVT.
Renner
W
W
Division of Angiology, Department of Medicine, University Hospital, Graz, Austria. wilfried.renner@kfunigraz.ac.at
Winkler
M
M
Hoffmann
C
C
Köppel
H
H
Seinost
G
G
Brodmann
M
M
Pilger
E
E
D000328:Adult; D000368:Aged; D016824:Antigens, Human Platelet / Q000235:genetics*; D001317:Austria; D016022:Case-Control Studies; D000939:Epitopes / Q000235:genetics; D005165:Factor V / Q000235:genetics; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D005838:Genotype; D006801:Humans; D039661:Integrin beta3; D008297:Male; D008875:Middle Aged; D019039:Platelet Glycoprotein GPIIb-IIIa Complex / Q000235:genetics*; D011110:Polymorphism, Genetic / Q000235:genetics; D011237:Predictive Value of Tests; D011516:Prothrombin / Q000235:genetics; D011655:Pulmonary Embolism / Q000235:genetics; D020246:Venous Thrombosis / Q000235:genetics*
D016824:Antigens, Human Platelet; D000939:Epitopes; C061248:ITGB3 protein, human; D039661:Integrin beta3; D019039:Platelet Glycoprotein GPIIb-IIIa Complex; C095381:factor V Leiden; C083451:human platelet antigen 1b; D005165:Factor V; D011516:Prothrombin