Relaxant effect of 6-deoxyclitoriacetal on smooth muscle preparations.
The pharmacological effect of 6-deoxyclitoriacetal (6-DA), a rotenoid compound isolated from the roots of Clitoria macrophylla Wall. (Papilionaceae), was examined on different smooth muscle preparations. 6-Deoxyclitoriacetal 0.2 mg/ml produced a significant decrease in the spontaneous contraction of isolated rat uterus. It also suppressed the contraction induced by acetylcholine 5x10(-6) M and oxytocin 5x10(-3) IU/ml. The cumulative contractile responses of rat aortic strips caused by serotonin 10(-8)-10(-4) M and norepinephrine 10(-11)-10(-7) M were reduced by 6-DA 0.4 mg/ml. In calcium free Kreb's solution, 6-DA inhibited the aortic contraction produced by a cumulative dose of calcium chloride (0.1-30 mM). In guinea-pig ileum, 6-DA 0.15 mg/ml exerted the spasmolytic activity by inhibition of the contractile response evoked by various contractile agents e.g. acetylcholine 10(-9)-10(-5) M, serotonin 10(-9)-10(-5) M and histamine 10(-9)-10(-5) M. All of the results indicated that 6-DA could induce a smooth muscle relaxant effect by interference with intracellular calcium metabolism.
Itthipanichpong
C
C
Department of Pharmacology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
Ruangrungsi
N
N
Saibundasak
K
K
D000818:Animals; D002478:Cells, Cultured; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005260:Female; D006168:Guinea Pigs; D007082:Ileum / Q000187:drug effects / Q000502:physiology; D066298:In Vitro Techniques; D008297:Male; D009126:Muscle Relaxation / Q000187:drug effects*; D009130:Muscle, Smooth / Q000166:cytology / Q000187:drug effects*; D009131:Muscle, Smooth, Vascular / Q000187:drug effects; D011336:Probability; D051381:Rats; D017208:Rats, Wistar; D012016:Reference Values; D012402:Rotenone / Q000031:analogs & derivatives / Q000494:pharmacology*; D012680:Sensitivity and Specificity; D014599:Uterus / Q000187:drug effects / Q000502:physiology
C436205:6-deoxyclitoriacetal; D012402:Rotenone; D004121:Dimethyl Sulfoxide