Glucose-6-phosphate dehydrogenase supports the functioning of the synapses in rat cerebellar cortex.
This study investigates heterogeneous glucose-6-phosphate dehydrogenase (G6PD) expression in the rat cerebellar cortex. G6PD activity and its electrophoretic pattern, evaluated on the cerebellar homogenate, were found to be similar to those of other brain areas. However, histochemical and immunohistochemical analyses revealed that the highest expression of G6PD activity and protein was in Purkinje's cells, followed by the molecular and granular layers. Electron microscopy analysis showed that, in Purkinje's cells, the G6PD reaction products were concentrated in the neurites while in the basket cells in the cell body. The granules showed a weaker activity everywhere. The quantitative distribution of G6PD is discussed in the light of the neurochemical function of these cells.
Biagiotti
E
E
Istituto di Chimica Biologica G. Fornaini, University of Urbino, Via A. Saffi 2, I-61029 (PU) Urbino, Italy.
Guidi
L
L
Capellacci
S
S
Ambrogini
P
P
Papa
S
S
Del Grande
P
P
Ninfali
P
P
D000818:Animals; D002525:Cerebellar Cortex / Q000201:enzymology* / Q000648:ultrastructure; D003687:Dehydroepiandrosterone; D004734:Energy Metabolism / Q000502:physiology*; D005947:Glucose / Q000378:metabolism*; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D008854:Microscopy, Electron; D009474:Neurons / Q000201:enzymology* / Q000648:ultrastructure; D011689:Purkinje Cells / Q000201:enzymology / Q000648:ultrastructure; D051381:Rats; D017207:Rats, Sprague-Dawley; D013053:Spectrophotometry; D013569:Synapses / Q000201:enzymology* / Q000648:ultrastructure; D009435:Synaptic Transmission / Q000502:physiology*
D003687:Dehydroepiandrosterone; D005954:Glucosephosphate Dehydrogenase; D005947:Glucose