title: Isolation and characterization of a species-specific DNA fragment for identification of Candida (Torulopsis) glabrata by PCR.
abstract: A PCR specific for Candida glabrata that amplifies a mitochondrial rRNA gene fragment was developed by analysis of C. glabrata-specific agarose gel bands, which were generated by arbitrarily primed PCR. The expected PCR product was successfully amplified with genomic DNA from 95 C. glabrata isolates but not from a number of other fungal isolates.
authors.affiliation: Institute of Medical Microbiology, University of Münster, D-48149 Münster, Germany. kbecker@uni-muenster.de
authors.forename: K
authors.initials: K
authors.lastname: Becker
authors.forename: D
authors.initials: D
authors.lastname: Badehorn
authors.forename: B
authors.initials: B
authors.lastname: Keller
authors.forename: M
authors.initials: M
authors.lastname: Schulte
authors.forename: K H
authors.initials: KH
authors.lastname: Böhm
authors.forename: G
authors.initials: G
authors.lastname: Peters
authors.forename: W
authors.initials: W
authors.lastname: Fegeler
mesh_terms: D002175:Candida / Q000145:classification* / Q000235:genetics*; D017931:DNA Primers; D004271:DNA, Fungal / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D004271:DNA, Fungal