F13459, a new derivative of mycophenolic acid. I. Taxonomy, isolation, and biological properties.
In the course of screening for inhibitors of intracellular trafficking of glycoprotein, a new inhibitor, F13459 was isolated from the culture broth of a Penicillium sp. It was purified using solvent extraction, silica gel, Sephadex LH-20 and ODS column chromatography. From structural analysis, F13459 was a derivative of mycophenolic acid, an inhibitor of inosine 5'-monophosphate dehydrogenase. F13459 inhibited hemagglutinin synthesis of NDV at concentrations more than 25 microg/ml. However, syncytium formation as a result of cell surface expression of F-glycoprotein of NDV was inhibited at concentrations of F13459 lower than those required for appreciable inhibition of glycoprotein synthesis.
Muroi
M
M
Animal and Cellular Systems Laboratory, RIKEN (The Institute of Physical and Chemical Research), Wako-shi, Saitama, Japan.
Sano
K
K
Okada
G
G
Koshino
H
H
Oku
T
T
Takatsuki
A
A
D000818:Animals; D000998:Antiviral Agents / Q000302:isolation & purification* / Q000494:pharmacology; D002478:Cells, Cultured; D006224:Cricetinae; D004791:Enzyme Inhibitors / Q000302:isolation & purification / Q000494:pharmacology; D015726:Giant Cells / Q000187:drug effects; D006388:Hemagglutinins / Q000096:biosynthesis; D007668:Kidney / Q000166:cytology; D008855:Microscopy, Electron, Scanning; D009173:Mycophenolic Acid / Q000031:analogs & derivatives* / Q000302:isolation & purification* / Q000494:pharmacology*; D009522:Newcastle disease virus / Q000187:drug effects / Q000378:metabolism; D010407:Penicillium / Q000737:chemistry* / Q000145:classification; D014760:Viral Fusion Proteins / Q000037:antagonists & inhibitors / Q000096:biosynthesis
D000998:Antiviral Agents; D004791:Enzyme Inhibitors; C434148:F13459; D006388:Hemagglutinins; D014760:Viral Fusion Proteins; D009173:Mycophenolic Acid