title: Interest of PS100 assay when (99m)Tc sestamibi scintigraphy failed to identify lymph node metastases of melanoma.
abstract: The study evaluated the contribution of serum PS100 assay to the detection of lymph node metastases during the follow-up of patients previously treated for a malignant melanoma, in addition to (99m)Tc sestamibi (MIBI) scintigraphy and investigation for gene MDR1, in order to detect chemoresistance phenomena. The study included 37 patients with a clinically questionable lymph node around basin lymphatic areas of the previously surgically-treated malignant melanoma. The sensitivity and specificity of PS100 assay were 91% and 86.5%, respectively. The sensitivity and specificity of MIBI scintigraphy were 95% and 85%, respectively. Overexpression of gene MDR1 was observed in six cases. In the event of negative scintigraphic findings, the concomitant analysis of PS100 levels and the scintigraphic result enabled the metastatic MDR+ patients to be distinguished from the non-metastatic patients. PS100 assay may therefore be proposed for the follow-up of malignant melanoma.
authors.affiliation: Laboratoire de Biologie du Tissus Osseux, Faculté de Médecine J.-Lisfranc, 42100 Saint-Etienne, France.
authors.forename: A
authors.initials: A
authors.lastname: Augusseau-Caillot
authors.forename: C
authors.initials: C
authors.lastname: Soler
authors.forename: F
authors.initials: F
authors.lastname: Teyssier
authors.forename: J L
authors.initials: JL
authors.lastname: Perrot
authors.forename: O
authors.initials: O
authors.lastname: Tiffet
authors.forename: F
authors.initials: F
authors.lastname: Cambazard
authors.forename: J
authors.initials: J
authors.lastname: Cuilleret
authors.forename: J M
authors.initials: JM
authors.lastname: Dumollard
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000235:genetics; D003937:Diagnosis, Differential; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008207:Lymphatic Metastasis / Q000000981:diagnostic imaging; D008545:Melanoma / Q000000981:diagnostic imaging / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011877:Radionuclide Imaging; D020133:Reverse Transcriptase Polymerase Chain Reaction; D009418:S100 Proteins / Q000032:analysis*; D012680:Sensitivity and Specificity; D012878:Skin Neoplasms / Q000000981:diagnostic imaging / Q000235:genetics / Q000378:metabolism*; D017256:Technetium Tc 99m Sestamibi
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D012333:RNA, Messenger; D009418:S100 Proteins; D017256:Technetium Tc 99m Sestamibi