Variability of haptoglobin and alpha 2-macroglobulin in Albino Oxford and Dark Augustin inbred strains of rats before and after trauma.
Increased synthesis of acute-phase proteins (APP) in the liver represents the most prominent aspect of acute phase reaction (APR). Haptoglobin (Hp) and alpha 2-macroglobulin (alpha 2-M) are among the most important APP in rats. In this study AO (Albino Oxford) and DA (Dark Augustin) inbred strains of rats, with different resistance to trauma, were used to compare concentrations of Hp, alpha 2-M, IL-1, IL-6 and TNF, before and after tourniquet trauma. Cytokines IL-1, IL-6 and TNF have an important role in APP gene expression. Our results showed that before trauma, more resistant AO rats had higher concentrations of Hp and alpha 2-M compared to DA rats. The influence of genetic elements of these differences is significant. Tourniquet trauma caused an early increase of IL-1, IL-6 and TNF activities in the serum which preceded the increase in the Hp and alpha 2-M concentrations but differences between strains were not found.
Strnad
M
M
Military Medical Academy, Institute of Pathology, Belgrade.
Radojicić
C
C
Marinković
D
D
Magić
Z
Z
D000818:Animals; D016207:Cytokines / Q000096:biosynthesis; D006242:Haptoglobins / Q000096:biosynthesis*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D014111:Tourniquets; D014947:Wounds and Injuries / Q000209:etiology / Q000378:metabolism*; D000511:alpha-Macroglobulins / Q000096:biosynthesis*
D016207:Cytokines; D006242:Haptoglobins; D000511:alpha-Macroglobulins