Bordetella bronchiseptica fimbrial protein-enhanced immunogenicity of a Mannheimia haemolytica leukotoxin fragment.
Leukotoxin produced by Mannheimia (Pasteurella) haemolytica is an important virulence factor in shipping fever pneumonia in feedlot cattle and is a critical protective antigen. In this study, the immune response to a chimeric protein generated by combining a gene fragment encoding neutralizing epitopes of M. haemolytica leukotoxin and a fimbrial protein gene (fim N) from Bordetella bronchiseptica was evaluated. The recombinant gene was cloned in a bacterial expression vector under the control of the tac promoter and expressed as a fusion protein with glutathione-S-transferase (GST) in Escherichia coli. Immunization of mice with the recombinant protein, GST-LTXFIM elicited a significantly stronger anti-leukotoxin antibody response than comparable immunizations with GST-LTX fusion proteins lacking FIM N. The GST-LTXFIM was also more stable than GST-LTX during storage at -80 degrees C, thus alleviating a stability problem inherent to leukotoxin. This chimeric protein may be a candidate for inclusion in new generation vaccines against shipping fever pneumonia.
Rajeev
S
S
Department of Comparative Medicine, College of Veterinary Medicine, University of Tennessee, Knoxville, TN 37996, USA.
Kania
S A
SA
Nair
R V
RV
McPherson
J T
JT
Moore
R N
RN
Bemis
D A
DA
D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis / Q000276:immunology; D000918:Antibody Specificity; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology*; D001426:Bacterial Proteins; D001428:Bacterial Vaccines / Q000276:immunology*; D016950:Bordetella bronchiseptica / Q000276:immunology*; D002417:Cattle; D002418:Cattle Diseases / Q000517:prevention & control; D000939:Epitopes / Q000276:immunology; D005098:Exotoxins / Q000235:genetics / Q000276:immunology*; D005260:Female; D036781:Fimbriae Proteins; D010861:Fimbriae, Bacterial / Q000276:immunology*; D005813:Genes, Synthetic; D005822:Genetic Vectors / Q000235:genetics; D005982:Glutathione Transferase / Q000235:genetics; D006460:Hemolysin Proteins / Q000235:genetics / Q000276:immunology*; D016978:Mannheimia haemolytica / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D012766:Pasteurellosis, Pneumonic / Q000517:prevention & control; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D013045:Species Specificity; D014614:Vaccines, Synthetic; D014774:Virulence; D010566:Virulence Factors, Bordetella
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D000939:Epitopes; D005098:Exotoxins; C417946:FimN protein, Bordetella bronchiseptica; D006460:Hemolysin Proteins; D011993:Recombinant Fusion Proteins; D014614:Vaccines, Synthetic; D010566:Virulence Factors, Bordetella; C102677:lktA protein, bacteria; D036781:Fimbriae Proteins; D005982:Glutathione Transferase