title: Low-intensity pulsed ultrasound stimulates a bone-forming response in UMR-106 cells.
abstract: Low-intensity (<100 mW/cm(2)) pulsed ultrasound (US) is an established therapy for fracture repair. In both animal and human trials, such US has been shown to facilitate fresh fracture repair and initiate healing in fractures with repair defects. However, the mechanism by which US achieves these outcomes is not clear. One possible mechanism is the direct stimulation of bone formation. To investigate this hypothesis, the current study investigated the mRNA response of isolated bone-forming cells (UMR-106 cells) to a single 20-min dose of low-intensity pulsed US. Using a novel US-cell coupling method, US was found to stimulate expression of the immediate-early response genes c-fos and COX-2 and elevate mRNA levels for the bone matrix proteins ALP and OC. These findings suggest that low-intensity pulsed US has a direct effect on bone formation. This may contribute to the beneficial effect of low-intensity pulsed US on fracture repair.
authors.affiliation: Centre for Sports Medicine Research and Education, School of Physiotherapy, University of Melbourne, Melbourne, Victoria, Australia.
authors.forename: S J
authors.initials: SJ
authors.lastname: Warden
authors.forename: J M
authors.initials: JM
authors.lastname: Favaloro
authors.forename: K L
authors.initials: KL
authors.lastname: Bennell
authors.forename: J M
authors.initials: JM
authors.lastname: McMeeken
authors.forename: K W
authors.initials: KW
authors.lastname: Ng
authors.forename: J D
authors.initials: JD
authors.lastname: Zajac
authors.forename: J D
authors.initials: JD
authors.lastname: Wark
mesh_terms: D000469:Alkaline Phosphatase / Q000096:biosynthesis / Q000235:genetics; D000818:Animals; D001861:Bone Regeneration; D051546:Cyclooxygenase 2; D017102:Fracture Healing; D017874:Immediate-Early Proteins / Q000096:biosynthesis / Q000235:genetics; D007334:Insulin-Like Growth Factor I / Q000096:biosynthesis / Q000235:genetics; D058574:Integrin-Binding Sialoprotein; D007527:Isoenzymes / Q000096:biosynthesis / Q000235:genetics; D007700:Kinetics; D010006:Osteoblasts / Q000502:physiology*; D015675:Osteocalcin / Q000096:biosynthesis / Q000235:genetics; D011451:Prostaglandin-Endoperoxide Synthases / Q000096:biosynthesis / Q000235:genetics; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D012795:Sialoglycoproteins / Q000096:biosynthesis / Q000235:genetics; D016212:Transforming Growth Factor beta / Q000096:biosynthesis / Q000235:genetics; D014407:Tumor Cells, Cultured; D014465:Ultrasonics
chemical_list: C546871:IBSP protein, human; D017874:Immediate-Early Proteins; D058574:Integrin-Binding Sialoprotein; D007527:Isoenzymes; D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger; D012795:Sialoglycoproteins; D016212:Transforming Growth Factor beta; D015675:Osteocalcin; D007334:Insulin-Like Growth Factor I; D051546:Cyclooxygenase 2; D011451:Prostaglandin-Endoperoxide Synthases; D000469:Alkaline Phosphatase