Detection of rpoB mutations in Mycobacterium tuberculosis by biprobe analysis.
A biprobe assay utilizing LightCycler technology was developed to detect rifampin resistance-associated gene mutations in the Mycobacterium tuberculosis rpoB gene. Three biprobes detected all mutations present in the 46 rifampin-resistant isolates. Wild-type sequences were correctly identified in each case. The method was reproducible, accurate, and easy to use.
Edwards
K J
KJ
Molecular Biology Unit, Virus Reference Division, Central Public Health Laboratory, Colindale, London, NW9 5HT, United Kingdom.
Metherell
L A
LA
Yates
M
M
Saunders
N A
NA
D000995:Antitubercular Agents / Q000494:pharmacology; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D024881:Drug Resistance, Bacterial; D024901:Drug Resistance, Multiple, Bacterial; D005456:Fluorescent Dyes / Q000378:metabolism*; D006801:Humans; D009154:Mutation; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D012293:Rifampin / Q000494:pharmacology; D012680:Sensitivity and Specificity; D018088:Tuberculosis, Multidrug-Resistant / Q000382:microbiology*
D000995:Antitubercular Agents; D005456:Fluorescent Dyes; D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit; D012293:Rifampin