title: The prognostic importance of the nucleolar organizer region (AgNOR), Ki-67 and proliferating cell nuclear antigen (PCNA) in primary nonurachal bladder adenocarcinoma.
abstract: The aim of this study was to investigate the role of tumor proliferation in patients with nonurachal bladder adenocarcinoma. Samples were obtained from 16 patients (12 men and 4 women, mean age 62 years) with primary nonrurachal bladder adenocarcinoma. The 16 formalin-fixed specimens were stained immunohistochemically for Ki-67 antigen and PCNA using MIB-1 and PC-10 antibodies. In addition, the AgNOR quantity was assessed using the colloid silver nitrate staining technique in all cases. The Ki-67, PCNA and AgNOR proliferation indices were found to be significantly higher in high-grade and invasive tumors. The higher the grade (p<0.01) and stage (p<0.01), the higher were the proliferation indices. Patients whose tumor samples had a high Ki-67, PCNA and AgNOR proliferation index showed a higher incidence of local recurrence (p<0.01) and distant metastasis (p<0.01). In conclusion, our results suggest that Ki-67, PCNA and AgNOR proliferation scores may be important prognostic indices in nonurachal bladder adenocarcinomas.
authors.affiliation: Ankara University School of Medicine, Department of Pathology, Turkey. handan27@hotmail.com
authors.forename: B H
authors.initials: BH
authors.lastname: Ozdemir
authors.forename: O G
authors.initials: OG
authors.lastname: Ozdemir
authors.forename: A
authors.initials: A
authors.lastname: Sertçelik
mesh_terms: D000230:Adenocarcinoma / Q000276:immunology* / Q000473:pathology* / Q000556:secondary; D000328:Adult; D000368:Aged; D002455:Cell Division; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local / Q000276:immunology / Q000473:pathology; D009367:Neoplasm Staging; D009697:Nucleolus Organizer Region / Q000473:pathology*; D011379:Prognosis; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism*; D001749:Urinary Bladder Neoplasms / Q000276:immunology* / Q000473:pathology*
chemical_list: D019394:Ki-67 Antigen; D018809:Proliferating Cell Nuclear Antigen