title: The antimutagenic effect of vanillin and cinnamaldehyde on spontaneous mutation in Salmonella TA104 is due to a reduction in mutations at GC but not AT sites.
abstract: Vanillin (VAN) and cinnamaldehyde (CIN) are dietary antimutagens that, when added to assay plates, reduced the spontaneous mutant frequency in Salmonella typhimurium strain TA104 (hisG428, rfa, uvrB, pKM101) by 50%. To date, no study has demonstrated whether or not the antimutagenic effects of an agent are due to a reduction in all classes of mutations or to a reduction in selective classes of mutations. To explore this issue, we have determined the spontaneous mutation spectrum in TA104 as well as the mutation spectrum after treatment of cells with antimutagens at concentrations that produced approximately a 50% reduction in mutant frequency but only a 10% reduction in survival. Statistical analysis revealed no significant difference between the mutation spectra of VAN- and CIN-treated cells. Relative to untreated cells, treatment with either VAN or CIN produced a significant reduction in mutations at GC sites, whereas neither compound produced a significant reduction in mutations at AT sites. Antimutagenesis experiments in hisG428 strains of Salmonella with varying DNA repair backgrounds showed that VAN and CIN require SOS repair genes to produce an antimutagenic effect against spontaneous mutagenesis. Studies evaluating the effect of VAN and CIN on growth rate showed that neither compound suppressed growth relative to untreated cells. To our knowledge, this is the first study to examine if an antimutagen reduced all or just some classes of mutations that were available for reduction.
authors.affiliation: Department of Environmental Science and Engineering, University of North Carolina, Chapel Hill, NC 27599, USA.
authors.forename: D T
authors.initials: DT
authors.lastname: Shaughnessy
authors.forename: R W
authors.initials: RW
authors.lastname: Setzer
authors.forename: D M
authors.initials: DM
authors.lastname: DeMarini
mesh_terms: D020856:AT Rich Sequence / Q000235:genetics; D000171:Acrolein / Q000031:analogs & derivatives* / Q000494:pharmacology*; D016587:Antimutagenic Agents / Q000494:pharmacology*; D001547:Benzaldehydes / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D004252:DNA Mutational Analysis; D004269:DNA, Bacterial / Q000187:drug effects / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D020862:GC Rich Sequence / Q000235:genetics; D016296:Mutagenesis / Q000187:drug effects*; D009152:Mutagenicity Tests; D012475:Salmonella / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism
chemical_list: D016587:Antimutagenic Agents; D001547:Benzaldehydes; D004269:DNA, Bacterial; D000171:Acrolein; C100058:vanillin; C012843:cinnamaldehyde