Effect of ischemia-reperfusion on bile canalicular F-actin microfilaments in hepatocytes of human liver allograft: image analysis by confocal laser scanning microscopy.
We studied and quantified the effect of ischemia-reperfusion on hepatic F-actin on bile canalicular and basolateral membranes in human liver allografts by means of confocal laser scanning microscopy imaging. The phalloidin-FITC staining of F-actin was normal in liver hepatocytes before reperfusion but decreased significantly after reperfusion (by 25% of controls). These results indicate that hepatic F-actin alteration is produced during the reperfusion phase. This modification, probably induced by reactive oxygen species, could impair bile canalicular contraction and tight junction permeability and consequently bile secretion in the postoperative period.
Benkoël
L
L
Laboratoire de Pathologie Cellulaire et Métabolique du Foie, INSERM U 559, Faculté de Médecine, Marseille, France.
Dodero
F
F
Hardwigsen
J
J
Campan
P
P
Botta-Fridlund
D
D
Lombardo
D
D
Le Treut
Y P
YP
Chamlian
A
A
D008841:Actin Cytoskeleton / Q000648:ultrastructure*; D000199:Actins / Q000648:ultrastructure*; D001648:Bile Canaliculi / Q000648:ultrastructure*; D001706:Biopsy; D002462:Cell Membrane / Q000648:ultrastructure; D004396:Coloring Agents; D016650:Fluorescein-5-isothiocyanate; D022781:Hepatocytes / Q000648:ultrastructure*; D006801:Humans; D016031:Liver Transplantation / Q000473:pathology*; D018613:Microscopy, Confocal; D010590:Phalloidine; D015427:Reperfusion Injury / Q000473:pathology*
D000199:Actins; D004396:Coloring Agents; D010590:Phalloidine; D016650:Fluorescein-5-isothiocyanate