title: Th2 cells support intrinsic anti-inflammatory properties of the brain.
abstract: In experimental autoimmune encephalomyelitis (EAE), Th1 cells are responsible for disease induction while Th2 cells can be protective. To address the mechanisms of this differential behavior, we utilized organotypic murine entorhinal-hippocampal slice cultures to analyze interactions between myelin basic protein-specific Th1 and Th2 cells with microglial cells. While both Th1 and Th2 cells induced CD40 expression, only Th1 cells induced intercellular adhesion molecule-1 (ICAM-1) expression on microglia. Moreover, Th2 cells prevented or even reversed Th1-induced ICAM-1 upregulation. Evidently, Th2 cells could diminish Th1-induced inflammatory reactions and actively support the resting state of microglia, which could be one mechanism of Th2-mediated remission of neuroinflammation during EAE.
authors.affiliation: Department of Cell and Neurobiology, Institute of Anatomy, Humboldt-University Clinic Charité, D-10098, Berlin, Germany. ulrike.gimsa@charite.de
authors.forename: U
authors.initials: U
authors.lastname: Gimsa
authors.forename: S A
authors.initials: SA
authors.lastname: Wolf
authors.forename: D
authors.initials: D
authors.lastname: Haas
authors.forename: I
authors.initials: I
authors.lastname: Bechmann
authors.forename: R
authors.initials: R
authors.lastname: Nitsch
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology; D019013:CD40 Antigens / Q000378:metabolism; D002450:Cell Communication; D002465:Cell Movement / Q000502:physiology; D016207:Cytokines / Q000096:biosynthesis; D004660:Encephalitis / Q000517:prevention & control*; D066298:In Vitro Techniques; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D051379:Mice; D008815:Mice, Inbred Strains; D017628:Microglia / Q000378:metabolism / Q000502:physiology; D018417:Th1 Cells / Q000502:physiology; D018418:Th2 Cells / Q000502:physiology*
chemical_list: D019013:CD40 Antigens; D016207:Cytokines; D018799:Intercellular Adhesion Molecule-1