title: Increased serum and salivary immunoglobulins against Candida albicans in HIV-infected patients with oral candidiasis.
abstract: The aim of this study was to explore anti-Candida albicans systemic and mucosal humoral responses against Candida virulence antigens such as somatic antigen and secreted aspartic proteases (Saps) in HIV-infected patients with oral candidiasis. Twenty-eight subjects were included in the study: 11 HIV-positive patients without oral candidiasis (group A), 6 HIV-positive patients with oral candidiasis (group B) and 11 HIV-negative healthy controls (group C). Total IgA, IgG and IgM concentrations and antibodies to C. albicans (somatic antigen, Sap1, Sap6) were measured in serum and saliva. We developed a time-resolved immunofluorometric assay with biotin and europium-labeled streptavidin for this purpose. Salivary total IgA, IgG and IgM concentrations were higher in group B. IgA, IgG and IgM anti-C. albicans antibodies (against somatic antigen, Sap1, Sap6) were higher in saliva and serum from patients from group B compared with patients from group A and controls. Our results suggest that, in oral candidiasis, HIV-infected patients have a high mucosal response, specifically directed against C. albicans virulence antigens, such as somatic antigen, Sap1 and Sap6.
authors.affiliation: Dermatology Department, St. Jacques Hospital, Besançon, France. christine.drobacheff@ufc-chu.univ-fcomte.fr
authors.forename: C
authors.initials: C
authors.lastname: Drobacheff
authors.forename: L
authors.initials: L
authors.lastname: Millon
authors.forename: M
authors.initials: M
authors.lastname: Monod
authors.forename: R
authors.initials: R
authors.lastname: Piarroux
authors.forename: E
authors.initials: E
authors.lastname: Robinet
authors.forename: R
authors.initials: R
authors.lastname: Laurent
authors.forename: D
authors.initials: D
authors.lastname: Meillet
mesh_terms: D017088:AIDS-Related Opportunistic Infections / Q000276:immunology*; D000328:Adult; D000908:Antibodies, Fungal / Q000097:blood* / Q000378:metabolism*; D000946:Antigens, Fungal; D016282:Aspartic Acid Endopeptidases / Q000276:immunology; D002176:Candida albicans / Q000201:enzymology / Q000276:immunology* / Q000472:pathogenicity; D002180:Candidiasis, Oral / Q000150:complications* / Q000276:immunology*; D016022:Case-Control Studies; D005260:Female; D015658:HIV Infections / Q000276:immunology; D006801:Humans; D018928:Immunity, Mucosal; D007070:Immunoglobulin A / Q000097:blood; D007071:Immunoglobulin A, Secretory / Q000378:metabolism; D007074:Immunoglobulin G / Q000097:blood / Q000378:metabolism; D007075:Immunoglobulin M / Q000097:blood / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D012463:Saliva / Q000276:immunology*
chemical_list: D000908:Antibodies, Fungal; D000946:Antigens, Fungal; D007070:Immunoglobulin A; D007071:Immunoglobulin A, Secretory; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D016282:Aspartic Acid Endopeptidases