title: PCR-based method for the construction of gene disruption cassettes in yeast.
authors.affiliation: Universidade do Minho Braga.
authors.forename: O
authors.initials: O
authors.lastname: Queirós
authors.forename: M
authors.initials: M
authors.lastname: Casal
authors.forename: P
authors.initials: P
authors.lastname: Moradas-Ferreira
authors.forename: C
authors.initials: C
authors.lastname: Leão
mesh_terms: D017931:DNA Primers / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics; D007716:Kluyveromyces / Q000235:genetics*; D026901:Membrane Transport Proteins / Q000235:genetics; D016254:Mutagenesis, Insertional / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D017931:DNA Primers; D005656:Fungal Proteins; D026901:Membrane Transport Proteins