Molecular diversity of live-attenuated prototypic vaccine strains and clinical isolates of Staphylococcus aureus.
Bovine mastitis Staphylococcus aureus isolates and prototypic live-attenuated vaccine strains were analyzed by SmaI pulsed-field gel electrophoresis (PFGE) typing and automated ribotyping. The discriminatory index of these methods was 0.91 and 0.69, respectively. SmaI PFGE typing assigned all laboratory strains into cluster Q, which shared 49% similarity with clusters A and B, and 35% similarity with cluster C. Automated ribotyping placed laboratory strains within ribogroups different from those of bovine isolates. These methods have 70% concordance and permitted identification of the prototypic vaccine background from those of clinical isolates. This information is required before conducting field trials with the vaccine.
Buzzola
F R
FR
Departamento de Microbiología, Facultad de Medicina, Universidad de Buenos Aires, Argentina.
Quelle
L S
LS
Steele-Moore
L
L
Berg
D
D
Denamiel
G
G
Gentilini
E
E
Sordelli
D O
DO
D000818:Animals; D002417:Cattle; D002986:Clinical Trials as Topic; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D019143:Evolution, Molecular; D005838:Genotype; D008414:Mastitis, Bovine / Q000382:microbiology*; D017720:Molecular Epidemiology / Q000379:methods / Q000592:standards; D015203:Reproducibility of Results; D015183:Restriction Mapping; D021521:Ribotyping / Q000379:methods* / Q000592:standards*; D013211:Staphylococcus aureus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D014613:Vaccines, Attenuated / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*
D004269:DNA, Bacterial; D014613:Vaccines, Attenuated