title: [Functional compartmentation of the translation apparatus and channeling of tRNA/aminoacyl-tRNA in cells of higher eukaryotes].
abstract: Literature data and authors' results in the field of structure-function organization of the translation apparatus in higher eukaryotes are considered. Proof is presented of the channeling of tRNA/aminoacyl-tRNA in the course of eukaryotic protein synthesis. The concept of the shuttle role of elongation factor eEF1A is grounded; the factor, being in a GTP-bound form, delivers aminoacyl-tRNA to the ribosome and then, in the GDP form after hydrolysis of GTP on the ribosome, forms a complex with the deacylated tRNA and delivers it to the aminoacyl-tRNA synthetase. The notion of translational compartment is defined.
authors.forename: B S
authors.initials: BS
authors.lastname: Negrutskiĭ
authors.forename: A V
authors.initials: AV
authors.lastname: El'skaia
mesh_terms: D000818:Animals; D001692:Biological Transport; D006153:Guanosine Diphosphate / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000378:metabolism; D006801:Humans; D020648:Peptide Elongation Factor 1 / Q000235:genetics / Q000378:metabolism; D014176:Protein Biosynthesis; D012343:RNA, Transfer / Q000235:genetics / Q000378:metabolism; D012346:RNA, Transfer, Amino Acyl / Q000235:genetics / Q000378:metabolism
chemical_list: D020648:Peptide Elongation Factor 1; D012346:RNA, Transfer, Amino Acyl; D006153:Guanosine Diphosphate; D006160:Guanosine Triphosphate; D012343:RNA, Transfer
vernacular_title: Funktsional'naia kompartmentalizatsiia apparata transliatsii i kanalizovannyĭ perenos tRNK/aminoatsil-tRNK v kletkakh vysshykh éukariot.