A comprehensive analysis of gene expression profiles in a yeast N-glycosylation mutant.
Although protein N-glycosylation is critical to many cell functions, its downstream targets remain largely unknown. In all eukaryotes, N-glycosylation utilizes the lipid-linked oligosaccharide (LLO) precursor, whose synthesis is initiated by the ALG7 gene. To elucidate the key signaling and metabolic events affected by N-glycosylation, we performed genomewide expression profiling of yeast cells carrying a hypomorphic allele of ALG7. DNA microarrays showed that of more than 97% of known or predicted yeast genes, 29 displayed increased expression while 23 were repressed in alg7 mutants. Changes in transcript abundance were observed for a and alpha mating-type genes, for genes functioning in several mitogen-activated protein kinase (MAPK) cascades, as well as in phosphate, amino acid, carbohydrate, mitochondrial and ATP metabolism. Therefore, DNA microarrays have revealed direct and indirect targets, including internal feedback loops, through which N-glycosylation affects signaling and metabolic activities and is functionally linked with cellular regulatory circuitry.
Klebl
B
B
Aventis Pharma GmbH Deutschland, Aventis Center for Functional Genomics, Fraunhoferstrasse 13, D-82152 Martinsried, Germany.
Kozian
D
D
Leberer
E
E
Kukuruzinska
M A
MA
D020413:3' Untranslated Regions; D000596:Amino Acids / Q000378:metabolism; D050260:Carbohydrate Metabolism; D020869:Gene Expression Profiling; D005800:Genes, Fungal; D049770:Genes, Mating Type, Fungal; D006031:Glycosylation; D020935:MAP Kinase Signaling System; D000072235:Mating Factor; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis; D010455:Peptides / Q000235:genetics / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism; D012331:RNA, Fungal / Q000096:biosynthesis; D017855:Transferases (Other Substituted Phosphate Groups) / Q000235:genetics* / Q000378:metabolism; D015003:Yeasts / Q000235:genetics* / Q000378:metabolism*
D020413:3' Untranslated Regions; D000596:Amino Acids; D010455:Peptides; D010710:Phosphates; D012331:RNA, Fungal; D000072235:Mating Factor; D017855:Transferases (Other Substituted Phosphate Groups); C023222:UDPacetylglucosamine-dolichyl-phosphate acetylglucosamine-1-phosphate transferase