title: Genetic diversity within and among Pinus pinaster populations: comparison between AFLP and microsatellite markers.
abstract: Twenty-three populations of Pinus pinaster (13 Aquitaine populations and 10 Corsican populations) were analysed at three microsatellite loci and 122 AFLP loci. The aims of the study were: (i) to compare levels of within-population and among-population diversity assessed with both kinds of markers; (ii) to compare Aquitaine and Corsican provenances of P. pinaster; and (iii) to know if both markers gave the same information for conservation purposes. Classical population genetics statistics were estimated and the ranking of populations obtained using different markers and/or parameters were compared by computing Spearman's rank correlations. Even though microsatellites showed a higher within-population diversity, they showed the same level of differentiation as AFLP markers. Moreover, both markers also showed a higher genetic diversity in the Aquitaine provenance and a higher differentiation among Corsican populations. AFLPs and microsatellites gave different population diversity rankings. Consequently, the results do not support the potential population identification within each provenance for conservation purposes.
authors.affiliation: INRA, Equipe de Génétique et Amélioration des Arbres Forestiers, BP45, F-33610 Cestas, France. stephanie.mariette@pierroton.inra.fr
authors.forename: S
authors.initials: S
authors.lastname: Mariette
authors.forename: D
authors.initials: D
authors.lastname: Chagné
authors.forename: C
authors.initials: C
authors.lastname: Lézier
authors.forename: P
authors.initials: P
authors.lastname: Pastuszka
authors.forename: A
authors.initials: A
authors.lastname: Raffin
authors.forename: C
authors.initials: C
authors.lastname: Plomion
authors.forename: A
authors.initials: A
authors.lastname: Kremer
mesh_terms: D000483:Alleles; D032462:Cycadopsida / Q000235:genetics*; D005602:France; D005787:Gene Frequency; D005819:Genetic Markers; D014644:Genetic Variation; D018895:Microsatellite Repeats; D014197:Trees / Q000235:genetics
chemical_list: D005819:Genetic Markers