title: Safety of ozonated solution as an antiseptic of the ocular surface prior to ophthalmic surgery.
abstract: OBJECTIVE
  To evaluate the safety of an ozonated solution as an antiseptic of the ocular surface prior to ophthalmic surgery.
  
  
  METHODS
  In experiment 1, a primary culture of rabbit corneal epithelium was established. Then, 0, 4 and 10 ppm ozonated solution and 1.25% povidone-iodine, respectively, were applied to confluent cells on collagen-coated filter inserts (Millicell-CM) for 10 min followed by replacement with fresh medium. The transepithelial electrical resistance (TER), which is a good indicator of cell barrier function, was sequentially measured for 30 min. In experiment 2, adult pigmented rabbit eyes were washed with 20 ml of 4 ppm ozonated solution, 1.25% povidone-iodine solution or saline. Slitlamp examinations were performed before and after washing.
  
  
  RESULTS
  In experiment 1, 4 ppm ozonated solution did not change the TER as compared with the control. 10 ppm ozonated solution and 1.25% povidone-iodine similarly reduced the TER values significantly as compared with those of the control and 4 ppm ozonated solution. In experiment 2, 4 ppm ozonated solution and saline showed mild superficial punctate keratitis (SPK) in 8.3% of eyes. However, 1.25% povidone-iodine resulted in mild SPK in 17% of eyes and moderate SPK in 25% of eyes. The prevalence of SPK between two groups was significantly different (p = 0.03).
  
  
  CONCLUSIONS
  Ozonated solution may be safe and a useful antiseptic of the ocular surface prior to ophthalmic surgery.
authors.affiliation: Department of Ophthalmology, Yamanashi Medical University, Yamanashi, Japan. kenjik@res.yamanashi-med.ac.jp
authors.forename: K
authors.initials: K
authors.lastname: Kashiwagi
authors.forename: K
authors.initials: K
authors.lastname: Saito
authors.forename: Y D
authors.initials: YD
authors.lastname: Wang
authors.forename: H
authors.initials: H
authors.lastname: Takahashi
authors.forename: K
authors.initials: K
authors.lastname: Ishijima
authors.forename: S
authors.initials: S
authors.lastname: Tsukahara
mesh_terms: D000818:Animals; D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D002462:Cell Membrane / Q000502:physiology; D002478:Cells, Cultured; D004553:Electric Conductivity; D017097:Electric Impedance; D019573:Epithelium, Corneal / Q000187:drug effects* / Q000378:metabolism; D005128:Eye Diseases / Q000601:surgery*; D015817:Eye Infections / Q000517:prevention & control; D009883:Ophthalmic Solutions / Q000494:pharmacology*; D010126:Ozone / Q000494:pharmacology*; D011206:Povidone-Iodine / Q000494:pharmacology; D011817:Rabbits; D012449:Safety
chemical_list: D000891:Anti-Infective Agents, Local; D009883:Ophthalmic Solutions; D010126:Ozone; D011206:Povidone-Iodine