Lack of DNA methylation in Schistosoma mansoni.
Schistosoma mansoni genomic DNA from male and female adult worms was subjected to restriction by the isoschizomeric endonucleases HpaII and MspI, which display different sensitivities with respect to cytosine methylation. The digested DNA was hybridized with 13 S. mansoni probes. Southern blot analysis showed that there were no observable differences in the restriction patterns of the two isoschizomers and that the patterns were identical in male and female parasites. Adenine methylation was also ruled out since no differences were observed with DpnI, Sau3A1, or MboI restriction enzymes. The methylation-dependent restriction endonuclease McrBC, which cleaves DNA containing methylcytosine and will not cleave unmethylated DNA, did not digest S. mansoni genomic DNA. These results demonstrate that the genome of adult S. mansoni is not methylated.
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Departamento de Bioquímica Médica ICB/CCS, Universidade Federal do Rio de Janeiro, Ilha do Fundão CEP 21941-590, Rio de Janeiro, Brazil.
Gimba
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Rumjanek
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D000818:Animals; D015139:Blotting, Southern; D053938:DNA Fragmentation; D019175:DNA Methylation; D018509:DNA, Helminth / Q000737:chemistry / Q000378:metabolism*; D005260:Female; D008297:Male; D015183:Restriction Mapping; D012550:Schistosoma mansoni / Q000235:genetics* / Q000378:metabolism
D018509:DNA, Helminth