title: Necrobiotic process causing burn wound conversion may be prevented by allogeneic keratinocytes delivered by the recombined human/pig skin.
abstract: The spontaneous necrobiotic process frequently causes conversion of DDB (deep 2nd degree wounds) into full-thickness skin loss (3rd degree wounds). We found that this process may be positively influenced by the activity of living human allogeneic keratinocytes cultured on acellular pig dermis. This RHPS, if applied 'upside-down' with the epidermal layer facing the wound, provides an opportunity for keratinocytes to influence the healing. The aim of the present study was to find conditions, in terms of timing and wound-bed preparation, for optimum healing activity of RHPS. The wound beds were prepared either with tangential excision, surface dermabrasion or deep dermabrasion. Out of 17 wounds grafted with RHPS after tangential excision, 15 (88%) healed in 4-10 days; early excised wounds (up to day 5) healed within less than 10 days after the injury. Out of 8 wounds grafted after surface dermabrasion, only 2 (25%) healed. Out of 6 wounds grafted with RHPS after deep dermabrasion, 4 (67%) healed. The optimum healing effect of RHPS and prevention of conversion was achieved in early tangentially excised wounds.
authors.affiliation: Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Prague. matous@img.cas.cz
authors.forename: E
authors.initials: E
authors.lastname: Matousková
authors.forename: L
authors.initials: L
authors.lastname: Broz
authors.forename: E
authors.initials: E
authors.lastname: Pokorná
authors.forename: R
authors.initials: R
authors.lastname: Königová
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D002056:Burns / Q000473:pathology* / Q000601:surgery; D002648:Child; D002675:Child, Preschool; D018920:Coculture Techniques; D003869:Dermabrasion; D020405:Dermis; D018450:Disease Progression; D005260:Female; D005500:Follow-Up Studies; D006133:Growth Substances / Q000378:metabolism; D006801:Humans; D007223:Infant; D015603:Keratinocytes / Q000378:metabolism / Q000637:transplantation*; D008297:Male; D009336:Necrosis; D016038:Skin Transplantation; D013552:Swine; D013997:Time Factors; D014182:Transplantation, Autologous; D014183:Transplantation, Heterologous; D014184:Transplantation, Homologous; D019737:Transplants; D016896:Treatment Outcome; D014945:Wound Healing
chemical_list: D006133:Growth Substances