Identification of polymorphisms within Disrupted in Schizophrenia 1 and Disrupted in Schizophrenia 2, and an investigation of their association with schizophrenia and bipolar affective disorder.
We have undertaken a search for polymorphic sequence variation within Disrupted in Schizophrenia 1 and Disrupted in Schizophrenia 2 (DISC1 and DISC2), which are both novel genes that span a translocation breakpoint strongly associated with schizophrenia and related psychoses in a large Scottish family. A scan of the coding sequence, intron/exon boundaries, and part of the 5' and 3' untranslated regions of DISC1, plus 2.7 kb at the 3' end of DISC2, has revealed a novel microsatellite and 15 novel single nucleotide polymorphisms (SNPs). We have tracked the inheritance of four of the SNPs through multiply affected families, and carried out case-control association studies using the microsatellite and four common SNPs on populations of patients with schizophrenia or bipolar affective disorder versus normal control subjects. Neither co-segregation with disease status nor significant association was detected; however, we could not detect linkage disequilibrium between all these markers in the control population, arguing that an even greater density of informative markers is required to test rigorously for association in this genomic region.
Devon
R S
RS
Medical Genetics Section, University of Edinburgh, Molecular Medicine Centre, Western General Hospital, UK. rebecca@cmmt.ubc.ca
Anderson
S
S
Teague
P W
PW
Burgess
P
P
Kipari
T M
TM
Semple
C A
CA
Millar
J K
JK
Muir
W J
WJ
Murray
V
V
Pelosi
A J
AJ
Blackwood
D H
DH
Porteous
D J
DJ
D000483:Alleles; D019943:Amino Acid Substitution; D001714:Bipolar Disorder / Q000453:epidemiology / Q000235:genetics*; D016022:Case-Control Studies; D002878:Chromosomes, Human, Pair 1 / Q000235:genetics* / Q000648:ultrastructure; D002880:Chromosomes, Human, Pair 11 / Q000235:genetics / Q000648:ultrastructure; D004252:DNA Mutational Analysis; D017931:DNA Primers; D005091:Exons / Q000235:genetics; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006239:Haplotypes / Q000235:genetics; D006801:Humans; D007438:Introns / Q000235:genetics; D015810:Linkage Disequilibrium; D018895:Microsatellite Repeats; D020125:Mutation, Missense; D009419:Nerve Tissue Proteins / Q000235:genetics*; D017354:Point Mutation; D011110:Polymorphism, Genetic; D018807:Polymorphism, Single-Stranded Conformational; D062085:RNA, Long Noncoding; D012333:RNA, Messenger; D012559:Schizophrenia / Q000453:epidemiology / Q000235:genetics*; D012606:Scotland / Q000453:epidemiology; D014178:Translocation, Genetic / Q000235:genetics*
C412476:DISC1 protein, human; C448722:DISC2 gene product, human; D017931:DNA Primers; D009419:Nerve Tissue Proteins; D062085:RNA, Long Noncoding; D012333:RNA, Messenger