title: [Study of the binding of the S7 protein with 16S rRNA fragment 926-986/1219-1393 as a key step in the assembly of the small subunit of prokaryotic ribosomes].
abstract: Both structural and thermodynamic studies are necessary to understand the ribosome assembly. An initial step was made in studying the interaction between a 16S rRNA fragment and S7, a key protein in assembling the prokaryotic ribosome small subunit. The apparent dissociation constant was obtained for complexes of recombinant Escherichia coli and Thermus thermophilus S7 with a fragment of the 3' domain of the E. coli 16S rRNA. Both proteins showed a high rRNA-binding activity, which was not observed earlier. Since RNA and proteins are conformationally labile, their folding must be considered to correctly describe the RNA-protein interactions.
authors.affiliation: Biology Departament, University of Virginia, Charlottesville, VA 22904-4775, USA.
authors.forename: T I
authors.initials: TI
authors.lastname: Rassokhin
authors.forename: A V
authors.initials: AV
authors.lastname: Golovin
authors.forename: E B
authors.initials: EB
authors.lastname: Petrova
authors.forename: V A
authors.initials: VA
authors.lastname: Spiridonova
authors.forename: O A
authors.initials: OA
authors.lastname: Karginova
authors.forename: T S
authors.initials: TS
authors.lastname: Rozhdestvenskiĭ
authors.forename: J
authors.initials: J
authors.lastname: Brosius
authors.forename: A M
authors.initials: AM
authors.lastname: Kopylov
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D011485:Protein Binding; D011487:Protein Conformation; D012329:RNA, Bacterial / Q000235:genetics / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012269:Ribosomal Proteins / Q000235:genetics / Q000378:metabolism*; D012270:Ribosomes / Q000235:genetics / Q000378:metabolism*; D016963:Thermus thermophilus / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D011994:Recombinant Proteins; D012269:Ribosomal Proteins; C037442:ribosomal protein S7
vernacular_title: Izuchenie sviazyvanie belka S7 s fragmentom 926-986/1219-1393 16S rRNK--kliuchevogo étapa sborki maloĭ subchastitsy prokarioticheskikh ribosom.