Synthesis and enzymatic evaluation of mucin type core 4 O-glycan.
Lactosylaminylated core-4 tetrasaccharide found in mucin type O-glycans has been synthesized . The non-reducing galactose residue of the deblocked tetrasaccharide was removed by beta-galactosidase from E. coli to produce the corresponding GlcNAc terminated compound. The core-2 and core-4 tetrasaccharides were evaluated as acceptors for the beta-1,3-N-acetylglucosaminyltransferase (iGnT), beta-1,4-Galactosyltransferase IV(beta4GalTIV) and beta-1,4-Galactosyltransferase I(beta4GalTI).
Misra
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The Burnham Institute, La Jolla, CA 92037, USA.
Ujita
M
M
Fukuda
M
M
Hindsgaul
O
O
D000818:Animals; D002240:Carbohydrate Sequence; D005700:Galactosyltransferases / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009077:Mucins / Q000138:chemical synthesis* / Q000737:chemistry; D017351:N-Acetylglucosaminyltransferases / Q000378:metabolism; D009237:N-Acetyllactosamine Synthase / Q000378:metabolism; D009844:Oligosaccharides / Q000138:chemical synthesis / Q000737:chemistry; D011134:Polysaccharides / Q000138:chemical synthesis* / Q000737:chemistry; D013379:Substrate Specificity
D009077:Mucins; D009844:Oligosaccharides; D011134:Polysaccharides; C106501:N-acetylglucosaminyltransferase IGnT; D005700:Galactosyltransferases; D017351:N-Acetylglucosaminyltransferases; C119701:beta-1,4-galactosyltransferase I; D009237:N-Acetyllactosamine Synthase