Dentate hilar mossy cells and somatostatin-containing neurons are immunoreactive for the alpha8 integrin subunit: characterization in normal and kainic acid-treated rats.
Integrins are heterodimeric cell surface receptors composed of different alpha and beta subunits that mediate cell-cell and cell-extracellular matrix interactions. They have been implicated in the regulation of neuronal migration, differentiation, process outgrowth, and plasticity. The alpha8 integrin subunit associates exclusively with the beta1 subunit to form a receptor (alpha8beta1) for fibronectin, vitronectin, tenascin, and osteopontin. In a previous study, we demonstrated that hippocampal dentate hilar neurons are immunoreactive for alpha8. The present study identifies the major types of alpha8-immunoreactive hilar neurons and characterizes the effects of kainic acid-induced seizures on alpha8-immunoreactivity in these cells. Examination of the hilus in normal rats revealed alpha8-immunoreactivity in the somatodendritic compartments of large hilar neurons identified as mossy cells, including a subset of dendritic thorny excrescences that were contacted by large mossy fiber terminals. alpha8-immunoreactivity also was found in approximately 71% of somatostatin-containing hilar cells. Kainic acid-induced seizures dramatically and rapidly altered the levels and distribution of alpha8-immunoreactivity in hilar neurons. After 1.5 h of seizures, alpha8-immunoreactivity in their dendrites was reduced greatly. One day after kainic acid treatment, labeling was diminished throughout the somatodendritic compartments of most hilar cells. This decrease appeared to be transient, since alpha8 labeling returned to normal levels in surviving hilar neurons within 2 weeks of treatment. In addition, many alpha8-immunoreactive hilar neurons, particularly in caudal dentate regions, were lost 3-5 weeks after kainic acid treatment. Our findings suggest that alpha8beta1 may mediate adhesive interactions of the dendritic processes of mossy cells and somatostatin-containing hilar neurons with other cellular elements or with extracellular matrix components. They also suggest that alpha8 may be susceptible to activity-dependent proteolysis that could modulate its function in the somatodendritic compartment of these cells.
Einheber
S
S
Department of Cell Biology, New York University School of Medicine, New York, NY 10016, USA. einhes01@med.nyu.edu
Pierce
J P
JP
Chow
D
D
Znamensky
V
V
Schnapp
L M
LM
Milner
T A
TA
D000818:Animals; D002448:Cell Adhesion / Q000502:physiology*; D002450:Cell Communication / Q000502:physiology; D002451:Cell Compartmentation / Q000502:physiology; D002452:Cell Count; D016923:Cell Death / Q000187:drug effects / Q000502:physiology; D003712:Dendrites / Q000378:metabolism* / Q000473:pathology / Q000648:ultrastructure; D018891:Dentate Gyrus / Q000378:metabolism* / Q000473:pathology / Q000648:ultrastructure; D004827:Epilepsy / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D018690:Excitatory Amino Acid Agonists / Q000494:pharmacology; D005109:Extracellular Matrix / Q000378:metabolism / Q000648:ultrastructure; D007150:Immunohistochemistry; D039001:Integrin alpha Chains; D016023:Integrins / Q000187:drug effects / Q000378:metabolism*; D007608:Kainic Acid / Q000494:pharmacology; D008297:Male; D008854:Microscopy, Electron; D019599:Mossy Fibers, Hippocampal / Q000378:metabolism* / Q000473:pathology / Q000648:ultrastructure; D009410:Nerve Degeneration / Q000139:chemically induced / Q000378:metabolism / Q000503:physiopathology; D010320:Parvalbumins / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D018091:Receptors, AMPA / Q000378:metabolism; D013004:Somatostatin / Q000378:metabolism*
D018690:Excitatory Amino Acid Agonists; D039001:Integrin alpha Chains; D016023:Integrins; D010320:Parvalbumins; D018091:Receptors, AMPA; C093856:integrin alpha8; D013004:Somatostatin; C104722:glutamate receptor ionotropic, AMPA 2; D007608:Kainic Acid