Photochemically induced equilibrium dysfunction in the hamster model with evaluation by means of a new globe rotatory test system.
Thrombosis in the inner ear is regarded as one of the causes of equilibrium dysfunction. We have established an experimental thrombosis model by producing a photochemical reaction between rose bengal and green light, and have evaluated the dysfunction with a new rotatory test system. Hamsters were treated with tissue-type plasminogen activator (tPA) in doses of 0, 0.13, 0.26, and 0.52 mg/kg. The equilibrium dysfunction of the hamsters was evaluated by scoring their behavior according to visual observation and by measuring their time on the rotatory test system. Treatment of animals with sufficient tPA (> or = 0.26 mg/kg) caused a significant amelioration of the behavior and a concomitant significant prolongation of time on the rotating globe. These findings suggest that the equilibrium dysfunction induced by the photochemical reaction was due to the thrombi formed, and that our test system may provide a useful tool for evaluating equilibrium dysfunction in hamsters.
Nishida
M
M
Department of Pharmacology, Gifu University School of Medicine, Japan.
Matsuno
H
H
Niwa
M
M
Kozawa
O
O
Ito
Y
Y
Miyata
H
H
Uematsu
T
T
D000818:Animals; D001522:Behavior, Animal; D006224:Cricetinae; D007758:Ear, Inner / Q000098:blood supply / Q000473:pathology; D005456:Fluorescent Dyes; D007759:Labyrinth Diseases / Q000150:complications / Q000188:drug therapy / Q000473:pathology; D008297:Male; D008647:Mesocricetus; D010777:Photochemistry; D004856:Postural Balance; D011994:Recombinant Proteins / Q000627:therapeutic use; D012395:Rose Bengal; D012399:Rotation; D012678:Sensation Disorders / Q000175:diagnosis* / Q000209:etiology; D013927:Thrombosis / Q000150:complications / Q000188:drug therapy / Q000473:pathology; D010959:Tissue Plasminogen Activator / Q000627:therapeutic use; D014172:Transillumination; D014724:Vestibular Function Tests / Q000295:instrumentation*
D005456:Fluorescent Dyes; D011994:Recombinant Proteins; D012395:Rose Bengal; D010959:Tissue Plasminogen Activator