title: The exon-exon junction complex provides a binding platform for factors involved in mRNA export and nonsense-mediated mRNA decay.
abstract: We recently reported that spliceosomes alter messenger ribonucleoprotein particle (mRNP) composition by depositing several proteins 20-24 nucleotides upstream of mRNA exon-exon junctions. When assembled in vitro, this so-called 'exon-exon junction complex' (EJC) contains at least five proteins: SRm160, DEK, RNPS1, Y14 and REF. To better investigate its functional attributes, we now describe a method for generating spliced mRNAs both in vitro and in vivo that either do or do not carry the EJC. Analysis of these mRNAs in Xenopus laevis oocytes revealed that this complex is the species responsible for enhancing nucleocytoplasmic export of spliced mRNAs. It does so by providing a strong binding site for the mRNA export factors REF and TAP/p15. Moreover, by serving as an anchoring point for the factors Upf2 and Upf3, the EJC provides a direct link between splicing and nonsense-mediated mRNA decay. Finally, we show that the composition of the EJC is dynamic in vivo and is subject to significant evolution upon mRNA export to the cytoplasm.
authors.affiliation: Howard Hughes Medical Institute, Department of Biochemistry, Brandeis University, 415 South Street, Waltham, MA 02454, USA.
authors.forename: H
authors.initials: H
authors.lastname: Le Hir
authors.forename: D
authors.initials: D
authors.lastname: Gatfield
authors.forename: E
authors.initials: E
authors.lastname: Izaurralde
authors.forename: M J
authors.initials: MJ
authors.lastname: Moore
mesh_terms: D000818:Animals; D034961:Antigens, Nuclear; D001665:Binding Sites; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000378:metabolism; D004926:Escherichia coli; D005091:Exons; D006801:Humans; D066298:In Vitro Techniques; D008957:Models, Genetic; D034521:Nuclear Matrix-Associated Proteins; D009687:Nuclear Proteins / Q000378:metabolism; D009865:Oocytes / Q000502:physiology; D012322:RNA Precursors / Q000378:metabolism; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D012261:Ribonucleoproteins; D014982:Xenopus laevis
chemical_list: D034961:Antigens, Nuclear; D004268:DNA-Binding Proteins; D034521:Nuclear Matrix-Associated Proteins; D009687:Nuclear Proteins; C406243:RBM8A protein, human; D012322:RNA Precursors; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; C084538:RNPS1 protein, human; D011994:Recombinant Proteins; D012261:Ribonucleoproteins; C111710:SRRM1 protein, human