Characterization by genotypic methods of symbiotic Nostoc strains isolated from five species of Gunnera.
The genetic diversity of ten symbiotic Nostoc strains isolated from different Gunnera species was investigated. The strains were analyzed using molecular methods with different taxonomic resolutions, including restriction fragment length polymorphisms (RFLP) of the PCR-amplified 16S ribosomal gene and the 16S-23S internal transcribed spacer (ITS) region combined with computer-assisted analyses. The functional gene hetR, assigned to heterocyst differentiation, was used for denaturing gradient gel electrophoresis. A high genetic diversity was observed among the isolates even in the conserved gene coding for the small ribosomal unit. No correlation was observed between clustering of cyanobacteria and the host species of Gunnera.
Rasmussen
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Department of Botany, Stockholm University, 10691 Stockholm, Sweden. ulla.rasmussen@botan.su.se
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D001426:Bacterial Proteins / Q000032:analysis; D015373:Bacterial Typing Techniques; D016000:Cluster Analysis; D000458:Cyanobacteria / Q000145:classification* / Q000166:cytology / Q000235:genetics; D004275:DNA, Ribosomal / Q000032:analysis / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D004586:Electrophoresis / Q000379:methods; D004587:Electrophoresis, Agar Gel; D010944:Plants / Q000382:microbiology*; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D013559:Symbiosis
D001426:Bacterial Proteins; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S; C067403:HetR protein, Bacteria