Association of induced disease resistance of rhododendron seedlings with inoculation of Streptomyces sp. R-5 and treatment with actinomycin D and amphotericin B to the tissue-culture medium.
Seedlings of rhododendron were treated by adding Streptomyces sp. strain R-5, actinomycin D and/or amphotericin B to the tissue-culture medium. HPLC analysis showed that all of the treated seedlings contained these antibiotics at concentrations higher than the suppressive levels to mycelial growth of Pestalotiopsis sydowiana, a major pathogen of rhododendron. Occurrence of disease caused by this fungus in the seedlings was suppressed by treatment of the medium surface with strain R-5, but not by treatment with these antibiotics, suggesting that growth of strain R-5, an antibiotic producer, could be essential for induction of disease resistance in tissue-cultured seedlings of rhododendron.
Shimizu
M
M
Laboratory of Ecological Circulation, Faculty of Bioresources, Mie University, Tsu-city, Japan.
Furumai
T
T
Igarashi
Y
Y
Onaka
H
H
Nishimura
T
T
Yoshida
R
R
Kunoh
H
H
D000666:Amphotericin B / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000935:Antifungal Agents / Q000494:pharmacology*; D046508:Culture Techniques; D003609:Dactinomycin / Q000494:pharmacology*; D019684:Magnoliopsida / Q000187:drug effects* / Q000382:microbiology*; D003904:Mitosporic Fungi; D009181:Mycoses / Q000188:drug therapy / Q000276:immunology / Q000517:prevention & control; D010935:Plant Diseases / Q000382:microbiology*; D013302:Streptomyces / Q000737:chemistry* / Q000378:metabolism
D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D003609:Dactinomycin; D000666:Amphotericin B