Effects of diphenhydramine HCl and methylprednisolone in the prevention of abdominal adhesions.
OBJECTIVE
The purpose of this study was to determine the effects of diphenhydramine hydrochloride and methylprednisolone in peritoneal adhesions.


METHODS
Forty-eight male rats were used in the study. The rats were anesthetized by 5 mg/kg ketamine hydrochloride. After opening the abdomen, 10 longitudinal incisions of 2 to 3 cm in length were made on the right parietal peritoneum, and a 2 cm(2) peritoneal layer was excised from the left abdominal wall. The abdomen was closed with 3/0 silk suture. Group I was the control group, group II was given 10 mg/kg diphenhydramine intravenously, group III was given 20 mg/kg methylprednisolone intravenously, and group IV was administered both of the drugs in the above doses. A blood sample of 2 mL was taken from the rats on the 14th day after the operation. The animals were then sacrificed. The abdomen was opened and abdominal adhesions were examined. A tissue sample of 1 g was taken from the abdominal incision line. Albumin, zinc, and hemoglobin levels and leucocyte counts in the blood were determined as well as hydroxyproline levels in the tissue.


RESULTS
Numbers of adhesions were as follows: 9 in group I, 3 in group II, and 2 in group III. No adhesion was observed in group IV. Albumin, zinc, and hemoglobin levels and leucocyte counts were found to be similar in all groups. Hydroxyproline levels in the tissue were significantly lower in groups III and IV than in groups I and II (P <0.05).


CONCLUSIONS
Diphenhydramine and methylprednisolone reduced postoperative adhesions significantly in rats. Further investigations are needed in order to use these drugs as antiadhesive agents in humans.
Avsar
F M
FM
Ankara Numune Training Research and Hospital, Clinic of 1st Surgery, Ankara, Turkey. fatihmehmet@superonline.com
Sahin
M
M
Aksoy
F
F
Avsar
A F
AF
Aköz
M
M
Hengirmen
S
S
Bilici
S
S
D000005:Abdomen / Q000601:surgery; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology / Q000627:therapeutic use*; D004155:Diphenhydramine / Q000494:pharmacology / Q000627:therapeutic use*; D004359:Drug Therapy, Combination; D006634:Histamine H1 Antagonists / Q000494:pharmacology / Q000627:therapeutic use*; D008297:Male; D008775:Methylprednisolone / Q000494:pharmacology / Q000627:therapeutic use*; D010532:Peritoneal Diseases / Q000517:prevention & control*; D011183:Postoperative Complications / Q000517:prevention & control*; D051381:Rats; D017208:Rats, Wistar; D018709:Statistics, Nonparametric; D000267:Tissue Adhesions
D000893:Anti-Inflammatory Agents; D006634:Histamine H1 Antagonists; D004155:Diphenhydramine; D008775:Methylprednisolone