ik3-1/Cables is associated with Trap and Pctaire2.
ik3-1/Cables is associated with and phosphorylated by cdk3 in self-replicating cells. In postmitotic neurons, it may serve as an adaptor molecule, functionally connecting c-abl and cdk5, and supporting neurite growth. Here, we cloned cDNAs coding for mouse Trap (tudor repeat associator with Pctaire 2) to interact with ik3-1. ik3-1 interacts with a region of mouse Trap containing the C-terminal tudor repeat domains 4 and 5 (corresponding to amino acids 881-1086 of mouse Trap). Furthermore, the N-terminal 93-amino-acid domain of ik3-1 is essential for ik3-1 interaction with Trap. Moreover, ik3-1 is coimmunoprecipitated with Pctaire 2 from COS7 cells, although we could not clarify whether ik3-1 is directly associated with Pctaire 2 or indirectly associated with Pctaire 2 through Trap. In vitro kinase assay indicated that ik3-1 does not activate phosphorylation of myelin basic protein or histione H 1 by the Pctaire 2-mediated kinase. These findings led us to speculate that through ik3-1, the Pctaire family and Trap may be functionally connected with cdk3 or cdk5.
Yamochi
T
T
Department of Pharmacology, KEIO University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo, 160-8582, Japan.
Nishimoto
I
I
Okuda
T
T
Matsuoka
M
M
D000595:Amino Acid Sequence; D000818:Animals; D019556:COS Cells; D002352:Carrier Proteins / Q000737:chemistry*; D003001:Cloning, Molecular; D051360:Cyclin-Dependent Kinase 5; D018844:Cyclin-Dependent Kinases / Q000737:chemistry / Q000378:metabolism; D016213:Cyclins / Q000737:chemistry*; D018076:DNA, Complementary / Q000378:metabolism; D004789:Enzyme Activation; D015723:Gene Library; D015151:Immunoblotting; D051379:Mice; D008957:Models, Genetic; D008969:Molecular Sequence Data; D004676:Myelin Basic Protein / Q000737:chemistry; D010750:Phosphoproteins / Q000737:chemistry*; D010766:Phosphorylation; D010957:Plasmids / Q000378:metabolism; D011233:Precipitin Tests; D011485:Protein Binding; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry*; D017434:Protein Structure, Tertiary; D016315:Proto-Oncogene Proteins c-abl / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014162:Transfection; D020798:Two-Hybrid System Techniques
C411580:Cables1 protein, mouse; D002352:Carrier Proteins; D016213:Cyclins; D018076:DNA, Complementary; D004676:Myelin Basic Protein; D010750:Phosphoproteins; D012333:RNA, Messenger; D016315:Proto-Oncogene Proteins c-abl; D051360:Cyclin-Dependent Kinase 5; D017346:Protein Serine-Threonine Kinases; C495906:Cdk5 protein, mouse; D018844:Cyclin-Dependent Kinases; C109207:PCTAIRE-2 protein kinase