title: Insulin-like growth factor-I gene expression patterns during spontaneous repair of acute articular cartilage injury.
abstract: This study evaluated the constitutive insulin-like growth factor-I (IGF-I) gene expression pattern in spontaneously healing cartilage defects over the course of 16 weeks, and correlated the tissue morphology and matrix gene expression with IGF-I mRNA levels. Full-thickness 15 mm cartilage defects were debrided in the femoral trochlea of both femoropatellar joints of 8 horses and the healing defects examined 2, 4, 8, or 16 weeks after surgery. Samples were harvested for histologic assessment of tissue healing using H&E staining, toluidine blue histochemical reaction for proteoglycan deposition, and in situ hybridization and immunohistochemistry procedures to demonstrate collagen type II mRNA and protein expression. Total RNA was isolated for Northern analysis to measure cartilage matrix molecule expression, and for semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) to determine IGF-I gene expression patterns in healing cartilage defects. Full-thickness cartilage defects in horses were slow to heal compared to smaller lesions in similar locations in other animals. However, a progressive decline in tissue cellularity and vascularity, and increased tissue organization were observed on H&E stained specimens over the 16-week experiment. Evidence of early chondrogenic repair was detected through collagen type II in situ hybridization and immunohistochemistry. However, levels of collagen type II and aggrecan mRNA in lesions were not abundant on Northern analysis indicating incomplete chondrogenesis. IGF-I message expression followed a cyclic pattern with low levels at 2 weeks, followed by an increase at 4 and 8 weeks, and a subsequent decline at 16 weeks. There was no direct correlation between the stage of healing and cartilage matrix message expression, and the abundance of IGF-I mRNA in the healing lesions. In conclusion, this study demonstrated that the spontaneous healing of articular defects was accompanied by a temporal fluctuation in IGF-I gene expression which was discoordinate to the steady rise in expression of cartilage matrix molecules such as procollagen type II.
authors.affiliation: Comparative Orthopaedics Laboratory, College of Veterinary Medicine, Cornell University, Ithaca, New York, USA.
authors.forename: L A
authors.initials: LA
authors.lastname: Fortier
authors.forename: C E
authors.initials: CE
authors.lastname: Balkman
authors.forename: L J
authors.initials: LJ
authors.lastname: Sandell
authors.forename: A
authors.initials: A
authors.lastname: Ratcliffe
authors.forename: A J
authors.initials: AJ
authors.lastname: Nixon
mesh_terms: D000208:Acute Disease; D053674:Aggrecans; D000818:Animals; D002358:Cartilage, Articular / Q000293:injuries* / Q000473:pathology / Q000502:physiology*; D019902:Chondrocytes / Q000502:physiology; D016326:Extracellular Matrix Proteins / Q000235:genetics; D015870:Gene Expression / Q000502:physiology; D006736:Horses; D007334:Insulin-Like Growth Factor I / Q000235:genetics*; D037181:Lectins, C-Type; D010641:Phenotype; D011347:Procollagen / Q000235:genetics; D011509:Proteoglycans / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D014945:Wound Healing / Q000502:physiology*
chemical_list: D053674:Aggrecans; D016326:Extracellular Matrix Proteins; D037181:Lectins, C-Type; D011347:Procollagen; D011509:Proteoglycans; D012333:RNA, Messenger; D007334:Insulin-Like Growth Factor I