title: Characterization of two novel Rhizobium leguminosarum bacteriophages from a field release site of genetically-modified rhizobia.
abstract: Two Rhizobium leguminosarum biovar viceae bacteriophages with contrasting properties were isolated from a field site in which the survival of genetically modified R. leguminosarum inoculants had been monitored for several years. Inoculant strain RSM2004 was used as the indicator for phage isolation and propagation. One phage, RL1RES, was temperate and could not replicate in any of the 42 indigenous R. leguminosarum field isolates tested although nested PCR indicated that phage sequences were present in six of the isolates. The second phage, RL2RES, was virulent, capable of generalised transduction, contained DNA with modified cytosine residues, and was capable of infecting all field isolates tested although the GM inoculant strain CT0370 was resistant. Sequence with homology to RL2RES was detected by nested PCR in six of the 42 field-isolates. These were not the same isolates that showed homology to RL1RES. The implication of these findings for the survival of rhizobial inoculants, and the ecology of phages and their host bacteria, are discussed.
authors.affiliation: Soil Science Department, IACR-Rothamsted, Harpenden, Hertfordshire, UK.
authors.forename: T A
authors.initials: TA
authors.lastname: Mendum
authors.forename: I M
authors.initials: IM
authors.lastname: Clark
authors.forename: P R
authors.initials: PR
authors.lastname: Hirsch
mesh_terms: D001435:Bacteriophages / Q000145:classification* / Q000235:genetics / Q000648:ultrastructure; D004279:DNA, Viral / Q000032:analysis; D005818:Genetic Engineering; D008242:Lysogeny; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D016961:Rhizobium leguminosarum / Q000235:genetics* / Q000254:growth & development / Q000821:virology*; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D014161:Transduction, Genetic
chemical_list: D004279:DNA, Viral