title: Variation of the sequence in the gene encoding for transmembrane protein M of canine coronavirus (CCV).
abstract: A nucleotide variability in the sequence of the gene encoding for the transmembrane protein M of canine coronavirus (CCV) is described. A total of 177 faecal samples from pups with enteritis were analysed by a PCR and n-PCR specific for CCV. Four samples, collected from a dog presenting a long-duration shedding of CCV, and a sample from another diarrhoeic dog, were found positive by PCR but negative by n-PCR. Sequence analysis of the samples revealed silent nucleotide substitutions in the binding site of the internal primer used for the n-PCR. Moreover, the nucleotide substitutions occurring over the whole fragment of the five samples analysed were similar.
authors.affiliation: Department of Health and Animal Well-being, Faculty of Veterinary Medicine, 70010 Valenzano, Bari, Italy. a.pratelli@veterinaria.uniba.it
authors.forename: A
authors.initials: A
authors.lastname: Pratelli
authors.forename: V
authors.initials: V
authors.lastname: Martella
authors.forename: G
authors.initials: G
authors.lastname: Elia
authors.forename: N
authors.initials: N
authors.lastname: Decaro
authors.forename: A
authors.initials: A
authors.lastname: Aliberti
authors.forename: D
authors.initials: D
authors.lastname: Buonavoglia
authors.forename: M
authors.initials: M
authors.lastname: Tempesta
authors.forename: C
authors.initials: C
authors.lastname: Buonavoglia
mesh_terms: D000818:Animals; D001483:Base Sequence; D018352:Coronavirus Infections / Q000662:veterinary / Q000821:virology; D000086862:Coronavirus M Proteins; D017939:Coronavirus, Canine / Q000235:genetics*; D003967:Diarrhea / Q000662:veterinary / Q000821:virology; D004283:Dog Diseases / Q000821:virology; D004285:Dogs; D005243:Feces / Q000821:virology; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics*; D016133:Polymerase Chain Reaction; D015203:Reproducibility of Results; D014763:Viral Matrix Proteins / Q000235:genetics*
chemical_list: D000086862:Coronavirus M Proteins; D014763:Viral Matrix Proteins