Determination of esmolol in serum by capillary zone electrophoresis and its monitoring in course of heart surgery.
A new Capillary Zone Electrophoresis (CZE) procedure for determination of esmolol, an ultra-short-acting beta-blocker, in serum was developed. Dichloromethane was applied as a deproteination agent and it was used also for the inactivation of erythrocytal esterase and in the same time for the extraction of esmolol from blood. The re-extraction of esmolol from organic phase to water phase was performed by 0.01 M HCl. An aliquot of 200 ml of acid aqueous phase was used for the injection and analysis. CZE determination was done in 50 mM phosphate buffer (pH=8.0) with detection at 222 nm. The concentration detection limit of esmolol in serum was 0.051 microg/ml. This method was applied in an extensive heart surgery experiment on pigs (Sus scrofa).
Malovaná
S
S
Department of Analytical Chemistry, Faculty of Science, Masaryk University, Brno, Czech Republic.
Gajdosová
D
D
Benedík
J
J
Havel
J
J
D000319:Adrenergic beta-Antagonists / Q000097:blood*; D016903:Drug Monitoring / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D011412:Propanolamines / Q000097:blood*; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D019616:Thoracic Surgical Procedures
D000319:Adrenergic beta-Antagonists; D011412:Propanolamines; C036604:esmolol