title: A simple and inexpensive "cell dissociation sieve-tissue grinder" apparatus.
abstract: A simple and inexpensive cell dissociation sieve-tissue grinder apparatus consisting essentially of stainless steel sieve (the one popularly used for sieving tea leaves) and a glass syringe plunger acting as pestle, is described for making single cell suspension.
authors.affiliation: Kala-azar Medical Research Centre, Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, India.
authors.forename: K
authors.initials: K
authors.lastname: Pai
authors.forename: S
authors.initials: S
authors.lastname: Sundar
mesh_terms: D000818:Animals; D002469:Cell Separation / Q000295:instrumentation*; D017364:Collagenases; D004867:Equipment Design; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000166:cytology; D014357:Trypsin
chemical_list: D014357:Trypsin; D017364:Collagenases