[Growth and metabolism of human skin fibroblasts cultured on microcarriers].
Human foreskin fibroblasts were cultured in vitro on microcarriers in spinners and traditional static flasks. The cultured cells obtained with these approaches were compared in cell shape, cell growth, cell production, and the metabolisms of glucose, lactate, and ammonium. The cells from microcarrier cultures through medium exchange were 8 times more than those from traditional static flasks. It was found that the specific growth rate and specific glucose consumption rate in microcarrier spinner cultures were 0.64/d-1 and 5.56 mmol/10(9) cell/d, respectively, higher than those in static flask cultures. However, the average lactate yield on glucose consumption in spinner cultures was only 0.955 mmol/mmol, lower than that in static flask cultures, 1. 125 mmol/mmol. This indicated that the energy metabolism in spinner cultures was significantly more efficient than that in static flasks. The experimental results from this work suggest that the microcarrier culture system is a suitable way to expand the seeding cells for tissue engineering, due to its ideal cultivation environment provided.
Deng
M A
MA
State Key Laboratory of Bioreactor Engineering, ECUST, Shanghai 200237, China.
Zhou
Y
Y
Hua
P
P
Tan
W S
WS
D002455:Cell Division; D002478:Cells, Cultured; D004734:Energy Metabolism; D005347:Fibroblasts / Q000502:physiology*; D006801:Humans; D012867:Skin / Q000166:cytology