Carotenoid incorporation into natural membranes from artificial carriers: liposomes and beta-cyclodextrins.
Liposomes and beta-cyclodextrin (beta-CD) have been used as carriers for the incorporation of three dietary carotenoids (beta-carotene (BC), lutein (LUT) and canthaxanthin (CTX)) into plasma, mitochondrial, microsomal and nuclear membrane fractions from pig liver cells or the retinal epithelial cell line D407. The uptake dynamics of the carotenoids from the carriers to the organelle membranes and their incorporation yield (IY) was followed by incubations at pH 7.4 for up to 3 h. The mean IYs saturated between 0.1 and 0.9 after 10-30 min of incubation, depending on membrane characteristics (cholesterol to phospholipid ratio) and carotenoid specificity. Mitochondrial membranes (more fluid) favour the incorporation of BC (non-polar), while plasma membranes (more rigid) facilitate the incorporation of lutein, the most polar carotenoid. A high susceptibility of BC to degradation in the microsomal suspension was observed by parallel incubations with/without 2,6-di-t-buthyl-p-cresol (BHT) as antioxidant additive. The beta-CD carrier showed to be more effective for the incorporation of lutein while BC was incorporated equally into natural membranes either from liposomes or from cyclodextrins. The presence of cytosol in the incubation mixture had no significant effects on the carotenoid incorporations.
Lancrajan
I
I
Department of Chemistry and Biochemistry, University of Agricultural Sciences and Veterinary Medicine, Cluj, Napoca, Romania.
Diehl
H A
HA
Socaciu
C
C
Engelke
M
M
Zorn-Kruppa
M
M
D000818:Animals; D001692:Biological Transport; D016644:Canthaxanthin / Q000737:chemistry / Q000493:pharmacokinetics; D002338:Carotenoids / Q000737:chemistry* / Q000493:pharmacokinetics*; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D003505:Cyclodextrins / Q000737:chemistry*; D004337:Drug Carriers; D006801:Humans; D007425:Intracellular Membranes / Q000378:metabolism*; D007700:Kinetics; D008081:Liposomes / Q000737:chemistry*; D008099:Liver / Q000378:metabolism*; D014975:Lutein / Q000737:chemistry / Q000493:pharmacokinetics; D008928:Mitochondria / Q000378:metabolism; D009685:Nuclear Envelope / Q000378:metabolism; D010857:Pigment Epithelium of Eye / Q000378:metabolism*; D013552:Swine; D019207:beta Carotene / Q000737:chemistry / Q000493:pharmacokinetics; D047392:beta-Cyclodextrins
D003505:Cyclodextrins; D004337:Drug Carriers; D008081:Liposomes; D047392:beta-Cyclodextrins; D019207:beta Carotene; D002338:Carotenoids; D016644:Canthaxanthin; C031215:betadex; D014975:Lutein