Identification of the green alga, Chlorella vulgaris (SDC1) using cyanobacteria derived 16S rDNA primers: targeting the chloroplast.
We have tested a set of oligonucleotide primers originally developed for the specific amplification of 16S rRNA gene segments from cyanobacteria, in order to determine their versatility as an identification tool for phototrophic eucaryotes. Using web-based bioinformatics tools we determined that these primers not only targeted cyanobacterium sequences as previously described, but also 87% of sequences derived from phototrophic eucaryotes. In order to qualify our finding, a type culture and environmental strain from the freshwater unicellular, green algae genus Chlorella Beijerinck, were selected for further study. Subsequently, we sequenced a 578-bp fragment of the 16S rRNA gene, which proved to be present within the chloroplast genome, performed sequence analysis and positively identified our solvent-degrading environmental strain (SDC1) as Chlorella vulgaris.
Burja
A M
AM
Department of Mechanical and Chemical Engineering, Heriot-Watt University, Riccarton, Edinburgh, Scotland, EH14 4AS, UK.
Tamagnini
P
P
Bustard
M T
MT
Wright
P C
PC
D002708:Chlorella / Q000145:classification* / Q000235:genetics; D002736:Chloroplasts / Q000235:genetics; D003001:Cloning, Molecular; D019295:Computational Biology; D017931:DNA Primers; D004271:DNA, Fungal / Q000235:genetics*; D005618:Fresh Water / Q000382:microbiology; D008969:Molecular Sequence Data; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid
D017931:DNA Primers; D004271:DNA, Fungal; D012336:RNA, Ribosomal, 16S