title: Fluidics cube for biosensor miniaturization.
abstract: To create a small, portable, fully automated biosensor, a compact means of fluid handling is required. We designed, manufactured, and tested a "fluidics cube" for such a purpose. This cube, made of thermoplastic, contains reservoirs and channels for liquid samples and reagents and operates without the use of any internal valves or meters; it is a passive fluid circuit that relies on pressure relief vents to control fluid movement. We demonstrate the ability of pressure relief vents to control fluid movement and show how to simply manufacture or modify the cube. Combined with the planar array biosensor developed at the Naval Research Laboratory, it brings us one step closer to realizing our goal of a handheld biosensor capable of analyzing multiple samples for multiple analytes.
authors.affiliation: Center for Bio/Molecular Science & Engineering, Naval Research Laboratory, Washington, DC 20375-5348, USA.
authors.forename: J M
authors.initials: JM
authors.lastname: Dodson
authors.forename: M J
authors.initials: MJ
authors.lastname: Feldstein
authors.forename: D M
authors.initials: DM
authors.lastname: Leatzow
authors.forename: L K
authors.initials: LK
authors.lastname: Flack
authors.forename: J P
authors.initials: JP
authors.lastname: Golden
authors.forename: F S
authors.initials: FS
authors.lastname: Ligler
mesh_terms: D015374:Biosensing Techniques; D004768:Enterotoxins / Q000032:analysis*; D005456:Fluorescent Dyes / Q000032:analysis
chemical_list: D004768:Enterotoxins; D005456:Fluorescent Dyes; C031818:enterotoxin B, staphylococcal