PLGA microparticles: possible vehicles for topical drug delivery.
Distribution of PLGA-microparticles in porcine skin, after its topical application, was studied in vitro using microparticles containing rhodamine as a fluorescent probe. PLGA-microparticles loaded with rhodamine were prepared using a solvent evaporation technique. Skin distribution of fluorescent microparticles was performed, by horizontal and vertical slicing of frozen skin. Fluorescence photomicrographs revealed that PLGA-microparticles could penetrate through the stratum corneum and reach the epidermis. However, permeation experiments showed that these microparticles were not able to reach the receptor compartment of the diffusion cells, even in a period of 24 h. The carriers described in this work could be used as vehicles for topical drug delivery, in order to obtain a sustained drug release into the skin, improving therapy by reduction of time intervals between doses.
de Jalón
E G
EG
Departamento de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia, Centro Galénico, Universidad de Navarra, 31080 Pamplona, Spain.
Blanco-Príeto
M J
MJ
Ygartua
P
P
Santoyo
S
S
D000287:Administration, Topical; D000818:Animals; D001672:Biocompatible Materials / Q000008:administration & dosage*; D016503:Drug Delivery Systems; D005456:Fluorescent Dyes / Q000494:pharmacology; D019344:Lactic Acid / Q000008:administration & dosage*; D010316:Particle Size; D011100:Polyglycolic Acid / Q000008:administration & dosage*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000008:administration & dosage*; D012235:Rhodamines / Q000494:pharmacology; D012867:Skin / Q000187:drug effects / Q000378:metabolism*; D013552:Swine
D001672:Biocompatible Materials; D005456:Fluorescent Dyes; D011108:Polymers; D012235:Rhodamines; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid