title: Subtyping and antifungal susceptibilities of Candida spp. in the intensive care unit of a Greek general hospital.
abstract: This study identified the Candida spp., susceptibility to antifungal agents and the prevailing Candida albicans subtypes responsible for infections or colonization of 42 patients in the ICU over a 6-month period. Most isolates were C. albicans (66.1%) and Candida tropicalis (28.3%) all of which were susceptible in vitro to antifungal agents. Subtypes of the C. albicans isolates were identified by pulsed field gel electrophoresis Sfi I chromosomal digests. Two major C. albicans subtypes were identified, whereas subtype heterogeneity was found among strains of Candida glabrata and C. tropicalis. Sfi I PFGE restriction patterns were able to discriminate between sub-populations of C. albicans isolates, clustering them into distinct, epidemiologically congruous groups.
authors.affiliation: Department of Microbiology, Sismanoglion District General Hospital, Greece.
authors.forename: M
authors.initials: M
authors.lastname: Kanellopoulou
authors.forename: G
authors.initials: G
authors.lastname: Stamos
authors.forename: I
authors.initials: I
authors.lastname: Petinnelli
authors.forename: M
authors.initials: M
authors.lastname: Savala
authors.forename: A
authors.initials: A
authors.lastname: Tzimogianni
authors.forename: N J
authors.initials: NJ
authors.lastname: Legakis
authors.forename: M
authors.initials: M
authors.lastname: Foustoukou
authors.forename: E
authors.initials: E
authors.lastname: Papafragas
authors.forename: A
authors.initials: A
authors.lastname: Velegraki
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D002175:Candida / Q000145:classification* / Q000187:drug effects* / Q000302:isolation & purification; D002177:Candidiasis / Q000382:microbiology*; D016521:Electrophoresis, Gel, Pulsed-Field; D006115:Greece; D006801:Humans; D007362:Intensive Care Units; D008826:Microbial Sensitivity Tests
chemical_list: D000935:Antifungal Agents