Stability of nitroblue tetrazolium-based alkaline phosphatase substrates.
This report demonstrates the stability of NBT substrate after multiple exposures to alkaline phosphatase. Perhaps more important than the ability to reuse substrates, the report provides some insight into the mechanisms by which tetrazoliums are reduced and evidence for the formation of an intermediary product, i.e., a half-formazan that is reduced more rapidly. (J Histochem Cytochem 49:1189-1190, 2001)
Smejkal
G B
GB
Department of Clinical Pathology, Cleveland Clinic Foundation, Cleveland, Ohio, USA. gary.smekjal@proteomesystems.com
Kaul
C A
CA
D000469:Alkaline Phosphatase / Q000737:chemistry*; D000906:Antibodies; D006801:Humans; D007202:Indicators and Reagents; D007211:Indoles / Q000737:chemistry; D008567:Membranes, Artificial; D009580:Nitroblue Tetrazolium / Q000737:chemistry*; D010084:Oxidation-Reduction; D012996:Solutions; D014841:von Willebrand Factor / Q000737:chemistry / Q000276:immunology
D000906:Antibodies; D007202:Indicators and Reagents; D007211:Indoles; D008567:Membranes, Artificial; D012996:Solutions; D014841:von Willebrand Factor; D009580:Nitroblue Tetrazolium; C035455:5-bromo-4-chloro-3-indoxyl phosphate; D000469:Alkaline Phosphatase