Rapid isolation and purification of 1-cyano-2-hydroxy-3-butene (crambene) from Crambe abyssinica seed meal using immiscible solvent extraction and high-performance liquid chromatography.
1-Cyano-2-hydroxy-3-butene (crambene) is a nitrile found in cruciferous vegetables that causes significant upregulation of quinone reductase and glutathione S-transferases in vivo and in vitro, making it a likely candidate as a cancer chemopreventive compound. To investigate further the putative anticarcinogenic mechanisms of crambene, a compound of the highest possible purity is vital. Therefore, a rapid and effective method of purification of crambene is necessary to continue studies of its beneficial health effects. A rapid method to isolate and purify natural crambene from either Crambe abyssinica (crambe) seed or commercially processed crambe seed meal was developed using immiscible solvent extraction followed by high-performance liquid chromatography. Use of this methodology eliminated the need for time-consuming and relatively inefficient column chromatography, improved extraction efficiency, and resulted in higher purity than previously used methodologies. Elimination of trace amounts of fatty acid residues, unachievable with previous methodologies, also was accomplished.
Niedoborski
T E
TE
Department of Food Science and Human Nutrition, Division of Nutritional Sciences, College of Agricultural, Consumer and Environmental Sciences, University of Illinois at Urbana-Champaign, 2001 South Lincoln Avenue, Urbana, Illinois 61802, USA.
Klein
B P
BP
Wallig
M A
MA
D000475:Alkenes / Q000302:isolation & purification*; D018890:Chemoprevention; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D009570:Nitriles / Q000302:isolation & purification*; D010936:Plant Extracts / Q000032:analysis*; D012639:Seeds / Q000737:chemistry*; D014675:Vegetables
D000475:Alkenes; D009570:Nitriles; D010936:Plant Extracts; C055903:1-cyano-2-hydroxy-3-butene