title: Clinical significance of oral urease in diagnosis of Helicobacter pylori infection by [13C]urea breath test.
abstract: This study was performed to evaluate the effect of oral flora on [13C]urea breath test in detecting H. pylori infection and find an optimal method and timing for sample collection. Forty-five volunteers were included in this study. The [13C]urea breath test was performed using mouthwash, endoscopic administration, and conventional methods. According to the receiver-operating characteristic curves, the earliest optimal time for discriminating H. pylori-positive and H. pylori-negative patients was at 25 min with the mouthwash method with 78% sensitivity and 82% specificity, at 2 min with the endoscopic administration method with 100% sensitivity and 100% specificity, and at 6 min with the conventional method with 100% sensitivity and 95% specificity. The study shows a significant effect of oral urease on the results of the [13C]urea breath test. The timing of sampling collection can be shortened to 6 min with the conventional method or to 2 min through endoscopic administration.
authors.affiliation: Department of Nuclear Medicine, Kaohsiung Veterans General Hospital, Taiwan.
authors.forename: N J
authors.initials: NJ
authors.lastname: Peng
authors.forename: K H
authors.initials: KH
authors.lastname: Lai
authors.forename: R S
authors.initials: RS
authors.lastname: Liu
authors.forename: S C
authors.initials: SC
authors.lastname: Lee
authors.forename: D G
authors.initials: DG
authors.lastname: Tsay
authors.forename: C C
authors.initials: CC
authors.lastname: Lo
authors.forename: H H
authors.initials: HH
authors.lastname: Tseng
authors.forename: W K
authors.initials: WK
authors.lastname: Huang
authors.forename: G H
authors.initials: GH
authors.lastname: Lo
authors.forename: P I
authors.initials: PI
authors.lastname: Hsu
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001944:Breath Tests / Q000379:methods; D002247:Carbon Isotopes; D005260:Female; D005773:Gastroscopy; D016481:Helicobacter Infections / Q000175:diagnosis* / Q000382:microbiology; D016480:Helicobacter pylori / Q000201:enzymology; D006801:Humans; D008297:Male; D008875:Middle Aged; D009055:Mouth / Q000201:enzymology / Q000382:microbiology*; D009067:Mouthwashes; D012372:ROC Curve; D012680:Sensitivity and Specificity; D014510:Urease / Q000032:analysis*
chemical_list: D002247:Carbon Isotopes; D009067:Mouthwashes; D014510:Urease