Sigma A recognition sites in the Bacillus subtilis genome.
A hidden Markov model of sigma(A) RNA polymerase cofactor recognition sites in Bacillus subtilis, containing either the common or the extended -10 motifs, has been constructed based on experimentally verified sigma(A) recognition sites. This work suggests that more information exists at the initiation site of transcription in both types of promoters than previously thought. When tested on the entire B. subtilis genome, the model predicts that approximately half of the sigma(A) recognition sites are of the extended type. Some of the response-regulator aspartate phosphatases were among the predictions of promoters containing extended sites. The expression of rapA and rapB was confirmed by site-directed mutagenesis to depend on the extended -10 region.
Jarmer
Hanne
H
Center for Biological Sequence Analysis, Building 2081, and Section for Molecular Microbiology, Building 3012, BioCentrum-DTU, Technical University of Denmark, DK-2800 Lyngby, Denmark.
Larsen
Thomas S
TS
Center for Biological Sequence Analysis, Building 2081, and Section for Molecular Microbiology, Building 3012, BioCentrum-DTU, Technical University of Denmark, DK-2800 Lyngby, Denmark.
Krogh
Anders
A
Center for Biological Sequence Analysis, Building 2081, and Section for Molecular Microbiology, Building 3012, BioCentrum-DTU, Technical University of Denmark, DK-2800 Lyngby, Denmark.
Saxild
Hans Henrik
HH
Center for Biological Sequence Analysis, Building 2081, and Section for Molecular Microbiology, Building 3012, BioCentrum-DTU, Technical University of Denmark, DK-2800 Lyngby, Denmark.
Brunak
Søren
S
Center for Biological Sequence Analysis, Building 2081, and Section for Molecular Microbiology, Building 3012, BioCentrum-DTU, Technical University of Denmark, DK-2800 Lyngby, Denmark.
Knudsen
Steen
S
Center for Biological Sequence Analysis, Building 2081, and Section for Molecular Microbiology, Building 3012, BioCentrum-DTU, Technical University of Denmark, DK-2800 Lyngby, Denmark.
D001412:Bacillus subtilis / Q000235:genetics* / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D016680:Genome, Bacterial; D008390:Markov Chains; D008957:Models, Genetic; D016297:Mutagenesis, Site-Directed; D010749:Phosphoprotein Phosphatases / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic; D012808:Sigma Factor / Q000378:metabolism*
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; C074434:RapA protein, E coli; D012808:Sigma Factor; C091155:rapB protein, Bacillus subtilis; D010749:Phosphoprotein Phosphatases