title: Existence of hepatitis C virus in Culex quinquefasciatus after ingestion of infected blood: experimental approach to evaluating transmission by mosquitoes.
abstract: We used PCR to detect hepatitis C virus (HCV) RNA among supernatants of ground Culex quinquefasciatus mosquitoes that (i) had been fed HCV-positive blood, (ii) had been intrathoracically inoculated with HCV-positive blood, or (iii) were from homes of hepatitis C patients. HCV RNA was detectable under all three conditions, but it did not replicate in mosquitoes and was not detectably transmitted during feeding.
authors.affiliation: Institute of Clinical Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan, Republic of China. ttchang@mail.ncku.edu.tw
authors.forename: T T
authors.initials: TT
authors.lastname: Chang
authors.forename: T Y
authors.initials: TY
authors.lastname: Chang
authors.forename: C C
authors.initials: CC
authors.lastname: Chen
authors.forename: K C
authors.initials: KC
authors.lastname: Young
authors.forename: J N
authors.initials: JN
authors.lastname: Roan
authors.forename: Y C
authors.initials: YC
authors.lastname: Lee
authors.forename: P N
authors.initials: PN
authors.lastname: Cheng
authors.forename: H L
authors.initials: HL
authors.lastname: Wu
mesh_terms: D000818:Animals; D001483:Base Sequence; D003465:Culex / Q000502:physiology / Q000821:virology*; D005247:Feeding Behavior; D005260:Female; D016174:Hepacivirus / Q000235:genetics / Q000302:isolation & purification*; D006526:Hepatitis C / Q000097:blood / Q000635:transmission* / Q000821:virology; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D012367:RNA, Viral / Q000032:analysis / Q000097:blood*; D017422:Sequence Analysis, DNA
chemical_list: D012367:RNA, Viral