title: Intermediates of yeast meiotic recombination contain heteroduplex DNA.
abstract: The formation of heteroduplex DNA features prominently in all models for homologous recombination. A central intermediate in the current double-strand break repair model contains two Holliday junctions flanking a region of heteroduplex DNA. Studies of yeast meiosis have identified such intermediates but failed to detect associated heteroduplex DNA. We show here that these intermediates contain heteroduplex DNA, providing an important validation of the double-strand break repair model. However, we also detect intermediates where both Holliday junctions are to one side of the initiating DSB site, while the intervening region shows no evidence of heteroduplex DNA. Such structures are not easily accommodated by the canonical version of the double-strand break repair model.
authors.affiliation: Laboratory of Biochemistry, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USA.
authors.forename: T
authors.initials: T
authors.lastname: Allers
authors.forename: M
authors.initials: M
authors.lastname: Lichten
mesh_terms: D004260:DNA Repair; D004271:DNA, Fungal / Q000378:metabolism; D008540:Meiosis; D009690:Nucleic Acid Conformation; D009692:Nucleic Acid Heteroduplexes / Q000378:metabolism*; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000502:physiology
chemical_list: D004271:DNA, Fungal; D009692:Nucleic Acid Heteroduplexes