Developmental analysis of a putative ATP/ADP carrier protein localized on glyoxysomal membranes during the peroxisome transition in pumpkin cotyledons.
In order to clarify the peroxisomal membrane proteins (PMPs), we characterized one of the major PMPs, PMP38. The deduced amino acid sequence for its cDNA in Arabidopsis thaliana contained polypeptides with 331 amino acids and had high similarity with those of Homo sapiens PMP34 and Candida boidinii PMP47 known as homologues of mitochondrial ATP/ADP carrier protein. We expected PMP38 to be localized on peroxisomal membranes, because it had the membrane peroxisomal targeting signal. Cell fractionation and immunocytochemical analysis using pumpkin cotyledons revealed that PMP38 is localized on peroxisomal membranes as an integral membrane protein. The amount of PMP38 in pumpkin cotyledons increased and reached the maximum protein level after 6 d in the dark but decreased thereafter. Illumination of the seedlings caused a significant decrease in the amount of the protein. These results clearly showed that the membrane protein PMP38 in glyoxysomes changes dramatically during the transformation of glyoxysomes to leaf peroxisomes, as do the other glyoxysomal enzymes, especially enzymes of the fatty acid beta-oxidation cycle, that are localized in the matrix of glyoxysomes.
Fukao
Y
Y
Department of Cell Biology, National Institute for Basic Biology, Okazaki, 444-8585 Japan.
Hayashi
Y
Y
Mano
S
S
Hayashi
M
M
Nishimura
M
M
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D016922:Cellular Senescence; D018548:Cotyledon / Q000254:growth & development / Q000378:metabolism*; D019662:Cucurbitaceae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D020676:Glyoxysomes / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D015151:Immunoblotting; D007425:Intracellular Membranes / Q000378:metabolism; D008027:Light; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism*; D063991:Mitochondrial Trifunctional Protein; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000378:metabolism; D020675:Peroxisomes / Q000235:genetics / Q000378:metabolism*; D010940:Plant Proteins; D021381:Protein Transport; D017386:Sequence Homology, Amino Acid
D002352:Carrier Proteins; D008565:Membrane Proteins; D009097:Multienzyme Complexes; D010940:Plant Proteins; C116693:peroxisomal membrane protein 34; C438006:peroxisomal membrane protein 38, plant; D063991:Mitochondrial Trifunctional Protein