title: Requirement of ceramide for adhesion of Helicobacter pylori to glycosphingolipids.
abstract: Direct adhesion of Helicobacter pylori to immobilized glycosphingolipids (GSLs) was compared to that of their corresponding oligosaccharide-conjugated neoglycoconjugates in order to clarify the roles of the carbohydrate and lipid portions of GSLs in H. pylori adhesion. These bacteria were found to adhere to sulfatide, GM3, GalCer and LacCer, but not to ceramide, sphingomyelin, or polyacrylamides conjugated with beta-galactose, lactose, 3'-sialyllactose or 3'-sulfo-beta-galactose. Furthermore, neoglycolipids or bovine serum albumin derivatives with corresponding oligosaccharides were unable to serve as the ligands. H. pylori adhesion to GalCer with alpha-hydroxyl fatty acid was much stronger than GalCer with the non-hydroxyl fatty acid. These results suggest that H. pylori recognize the conformation of GSL with alpha-hydroxyl fatty acid on solid phase.
authors.affiliation: Department of Applied Biochemistry, Tokai University, Hiratsuka, Kanagawa, Japan.
authors.forename: W
authors.initials: W
authors.lastname: Tang
authors.forename: K
authors.initials: K
authors.lastname: Seino
authors.forename: M
authors.initials: M
authors.lastname: Ito
authors.forename: T
authors.initials: T
authors.lastname: Konishi
authors.forename: H
authors.initials: H
authors.lastname: Senda
authors.forename: M
authors.initials: M
authors.lastname: Makuuchi
authors.forename: N
authors.initials: N
authors.lastname: Kojima
authors.forename: T
authors.initials: T
authors.lastname: Mizuochi
mesh_terms: D001422:Bacterial Adhesion / Q000502:physiology*; D002518:Ceramides / Q000502:physiology*; D006028:Glycosphingolipids / Q000378:metabolism / Q000502:physiology*; D016480:Helicobacter pylori / Q000502:physiology*; D009844:Oligosaccharides / Q000378:metabolism
chemical_list: D002518:Ceramides; D006028:Glycosphingolipids; D009844:Oligosaccharides