[Cytotoxic T lymphocyte activity induced by genetic immunization with NV-HC/NS].
OBJECTIVE
To investigate the immunoprophylactic and immunotherapeutic effects of DNA vaccine (NV-HC/NS(3)) after inoculation of mice by target tumor loading technique.


METHODS
Before or after inoculation with NV-HC/NS(3) intramuscularly, BALB/c mice in the experimental group were planted with target tumor cells (SP2/0-NS(3)), which were derived from BALB/c mice and stably transfected with the recombinant plasmid NV-HC/NS(3). Tumor growth and survival rate of the mice immunized with NV-HC/NS(3) were compared with those in the control group.


RESULTS
Tumor mass grew well in all mice in the control group in a few days, while no visible tumor mass growth in 40% mice immunized with NV-HC/NS(3) could be seen. The survival rate and life span of mice in the experimental group were significantly longer than those in the control group.


CONCLUSIONS
Specific immune activity could be induced by immunization with NV-HC/NS(3). Such effects are immunoprophylactic and immunotherapeutic.
Zhao
L
L
Key Laboratory of Molecular Biology for Infectious Diseases of Sichuan Province, First University Hospital, West China Medical Center, Sichuan University, Chengdu 610041, China.
Zhou
T
T
Qin
S
S
Liu
L
L
Liu
C
C
Tang
H
H
Lei
B
B
D000818:Animals; D004279:DNA, Viral / Q000276:immunology* / Q000627:therapeutic use; D004195:Disease Models, Animal; D005260:Female; D016174:Hepacivirus / Q000235:genetics / Q000276:immunology; D007114:Immunization; D007167:Immunotherapy; D051379:Mice; D008807:Mice, Inbred BALB C; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000276:immunology / Q000401:mortality / Q000517:prevention & control*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D019444:Vaccines, DNA / Q000627:therapeutic use*
D004279:DNA, Viral; D019444:Vaccines, DNA