Infection of synoviocytes with HTLV-I induces telomerase activity.
To investigate the mechanism of synovial hyperplasia by human T-lymphotropic virus type I (HTLV-I) infection, the enzymatic activity of telomerase and expression of telomerase-related factors in HTLV-I infected synoviocytes were examined. Cultured synoviocytes obtained from four patients with osteoarthritis (OA) and four with traumatic joint disease (TJD) were infected by HTLV-I. Telomerase activity was detected by telomeric repeat amplification protocol (TRAP) assay. Expression of telomerase-related mRNAs such as telomerase reverse transcriptase (hTERT), telomerase RNA component (hTERC), and telomeric repeat binding factor 2 (TRF2) were also examined. Telomerase activity was detected in all HTLV-I-infected synoviocytes but not in uninfected synoviocytes. A remarkable induction of hTERT mRNA was observed in four of eight HTLV-I-infected synoviocytes, whereas expressions of hTERC, TRF2, and TEP-1 mRNAs were not changed. Our results clearly demonstrate that HTLV-I upregulates telomerase activity in synoviocytes probably via upregulation of hTERT activity. These findings suggest that telomerase activation in synoviocytes has an important role in upregulated proliferative activity of HAAP synoviocytes.
Tsumuki
H
H
Rheumatology, Immunology, and Genetics Program, Institute of Medical Science, St Marianna University School of Medicine, Kawasaki, Japan.
Nakazawa
M
M
Hasunuma
T
T
Kobata
T
T
Kato
T
T
Uchida
A
A
Nishioka
K
K
D002352:Carrier Proteins / Q000096:biosynthesis / Q000235:genetics; D002455:Cell Division; D002478:Cells, Cultured; D018920:Coculture Techniques; D017931:DNA Primers / Q000737:chemistry; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics; D015368:Human T-lymphotropic virus 1 / Q000502:physiology*; D006801:Humans; D020370:Osteoarthritis, Knee / Q000473:pathology; D012313:RNA; D062085:RNA, Long Noncoding; D012333:RNA, Messenger / Q000378:metabolism; D022661:RNA, Untranslated / Q000096:biosynthesis / Q000235:genetics; D016601:RNA-Binding Proteins; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013583:Synovial Membrane / Q000201:enzymology / Q000821:virology*; D019098:Telomerase / Q000096:biosynthesis* / Q000235:genetics; D035341:Telomeric Repeat Binding Protein 2; D015854:Up-Regulation
D002352:Carrier Proteins; D017931:DNA Primers; D004268:DNA-Binding Proteins; D062085:RNA, Long Noncoding; D012333:RNA, Messenger; D022661:RNA, Untranslated; D016601:RNA-Binding Proteins; C104342:TEP1 protein, human; D035341:Telomeric Repeat Binding Protein 2; C422952:telomerase RNA; D012313:RNA; D019098:Telomerase