title: Levels of leukotriene B4 in gingival crevicular fluid and gingival tissue in specific periodontal diseases.
abstract: BACKGROUND
  Leukotriene B4 (LTB4), a product of the lipoxygenase pathway of arachidonic acid metabolism, exhibits numerous activities that can account for most of the features of host responses seen in periodontal diseases. The aim of the present study was to examine the role of LTB4 in the pathogenesis of specific periodontal diseases.
  
  
  METHODS
  LTB4 levels were investigated in gingival crevicular fluid (GCF) and gingival tissue (GT) samples of 10 patients with chronic periodontitis (CP), 12 patients with generalized aggressive periodontitis (GAgP), 6 patients with localized aggressive periodontitis (LAgP), 6 patients with gingivitis (G), and 6 periodontally healthy subjects (H). Periodontal status was evaluated by measuring probing depth, gingival index, papillary bleeding index, and plaque index. LTB4 was extracted from the samples by solid-phase method using C18 cartridge and was purified by high performance liquid chromatographic method and then analyzed by radioimmunoassay.
  
  
  RESULTS
  All patient groups had significantly higher levels of GCF and GT LTB4 compared to the control group (P<0.005). The CP patients had the highest LTB4 levels compared to those in other patient groups (P<0.005). GAgP, LAgP, and G groups had similar amounts of GCF and GT LTB4 (P>0.005). When the data were expressed as concentration, the CP group was found to have higher concentration of LTB4, compared to that of control group (P<0.005). GAgP, LAgP, and G groups had similar LTB4 concentration compared to that of control group (P>0.005). No significant difference was found between GAgP, LAgP, and G groups (P>0.005). The CP group had higher LTB4 concentration compared to both GAgP and LAgP groups (P<0.005). Although the CP group had a higher GCF LTB4 concentration compared to G group, this difference did not reach significance (P>0.005). No significant correlation was found between GCF and GT LTB4 levels and clinical parameters.
  
  
  CONCLUSIONS
  The results of the present study indicate that LTB4 is likely to be an important mediator in regulating inflammatory responses in the human periodontal tissues. This lipid mediator may play an important role in the pathophysiology of periodontal disease.
authors.affiliation: Ege University, School of Dentistry, Department of Periodontology, Izmir, Turkey. emingil@usa.net
authors.forename: G
authors.initials: G
authors.lastname: Emingil
authors.forename: S
authors.initials: S
authors.lastname: Cinarcik
authors.forename: H
authors.initials: H
authors.lastname: Baylas
authors.forename: I
authors.initials: I
authors.lastname: Coker
authors.forename: A
authors.initials: A
authors.lastname: Hüseyinov
mesh_terms: D000328:Adult; D010520:Aggressive Periodontitis / Q000276:immunology / Q000378:metabolism; D016022:Case-Control Studies; D002851:Chromatography, High Pressure Liquid; D002908:Chronic Disease; D005260:Female; D005881:Gingiva / Q000737:chemistry / Q000378:metabolism*; D005883:Gingival Crevicular Fluid / Q000737:chemistry / Q000378:metabolism*; D005891:Gingivitis / Q000276:immunology / Q000378:metabolism; D006801:Humans; D007975:Leukotriene B4 / Q000032:analysis / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D010518:Periodontitis / Q000276:immunology / Q000378:metabolism*; D011863:Radioimmunoassay; D018709:Statistics, Nonparametric
chemical_list: D007975:Leukotriene B4