The contribution of plastidial phosphoglucomutase to the control of starch synthesis within the potato tuber.
The aim of this work was to evaluate the extent to which plastidial phosphoglucomutase (PGM) activity controls starch synthesis within potato (Solanum tuberosum L. cv. Desirée) tubers. The reduction in the activity of plastidial PGM led to both a correlative reduction in starch accumulation and an increased sucrose accumulation. The control coefficient of plastidial PGM on the accumulation of starch was estimated to approximate 0.24. The fluxes of carbohydrate metabolism were measured by investigating the metabolism of [U-14C]glucose in tuber discs from wild-type and transgenic plants. In tuber discs the control coefficient of plastidial PGM over starch synthesis was estimated as 0.36, indicating that this enzyme exerts considerable control over starch synthesis within the potato tuber.
Fernie
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AR
Max-Planck-Institut für Molekulare Pflanzenphysiologie, Golm, Germany. fernie@mpimp-golm.mpg.de
Roessner
U
U
Trethewey
R N
RN
Willmitzer
L
L
D000596:Amino Acids / Q000032:analysis; D002250:Carbon Radioisotopes; D003600:Cytosol / Q000378:metabolism; D005947:Glucose / Q000032:analysis / Q000378:metabolism*; D066298:In Vitro Techniques; D010733:Phosphoglucomutase / Q000378:metabolism*; D018514:Plant Structures / Q000201:enzymology; D030821:Plants, Genetically Modified; D018087:Plastids / Q000378:metabolism; D011198:Solanum tuberosum / Q000201:enzymology*; D013213:Starch / Q000032:analysis / Q000096:biosynthesis*; D013395:Sucrose / Q000032:analysis / Q000378:metabolism*
D000596:Amino Acids; D002250:Carbon Radioisotopes; D013395:Sucrose; D013213:Starch; D010733:Phosphoglucomutase; D005947:Glucose