Astilbin selectively facilitates the apoptosis of interleukin-2-dependent phytohemagglutinin-activated Jurkat cells.
The present study examined the relationship between the activation of T cells and the apoptosis-facilitating effect of astilbin on them. By the stimulation of PHA, a remarkable IL-2 production was detected in the supernatant of Jurkat cells after 120 h among 72--144 h incubation. This kinetics was quite in accordance with that of astilbin-induced apoptosis of Jurkat cells, where 1 h-exposure of the PHA-activated cells to astilbin caused a significantly increased apoptosis in a dose-dependent manner. To the Jurkat cells that had been cultivated for 72--144 h without PHA, however, astilbin did not show any facilitation of the cell apoptosis. Pre-treatment by cyclosporine A simultaneously with PHA dose-dependently lowered the IL-2 production and susceptibility of the cells to astilbin, while the treatment after 120 h of PHA-activation did not. The exogenous IL-2 treatment after 72 h of PHA-activation significantly and dose-dependently raised the susceptibility of the Jurkat cells to astilbin. These results indicated the dependency of the apoptosis-facilitating effect of astilbin on appropriate status of activated T lymphocytes with a relation to IL-2 production. This characteristic of astilbin may be of great significance for the treatment of a variety of immunologically related diseases.
Yan
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R
Department of Pharmacology for Chinese Materia Medica, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing 210009, China.
Xu
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D000975:Antioxidants / Q000494:pharmacology; D017209:Apoptosis; D016572:Cyclosporine / Q000494:pharmacology; D004347:Drug Interactions; D005419:Flavonoids / Q000494:pharmacology*; D044948:Flavonols; D006801:Humans; D007376:Interleukin-2 / Q000378:metabolism*; D019169:Jurkat Cells; D008213:Lymphocyte Activation / Q000187:drug effects*; D010835:Phytohemagglutinins / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000187:drug effects* / Q000473:pathology
D000975:Antioxidants; D005419:Flavonoids; D044948:Flavonols; D007376:Interleukin-2; D010835:Phytohemagglutinins; C099069:astilbin; D016572:Cyclosporine