title: Protection of radiation-induced protein damage by curcumin.
abstract: Free radical reactions of lysozyme (Lz), tryptophan and disulfides were studied with curcumin, a lipid-soluble antioxidant from turmeric, in aqueous solution using a pulse radiolysis technique. The binding of curcumin with lysozyme was confirmed using absorption, fluorescence and stopped-flow techniques. The free radicals of curcumin generated after repairing radicals of disulfides, lysozyme and tryptophan absorb at 500-510 nm. Implication of this in evaluating the antioxidant behavior of curcumin in protecting proteins is discussed.
authors.affiliation: Radiation Chemistry and Chemical Dynamics Division, Bhabha Atomic Research Center, Mumbai 400 085, India. sudhirk@apsara.barc.ernet.in
authors.forename: S
authors.initials: S
authors.lastname: Kapoor
authors.forename: K I
authors.initials: KI
authors.lastname: Priyadarsini
mesh_terms: D000465:Algorithms; D016880:Anisotropy; D000975:Antioxidants / Q000494:pharmacology; D003474:Curcumin / Q000494:pharmacology*; D004220:Disulfides / Q000737:chemistry; D005609:Free Radicals / Q000737:chemistry; D009113:Muramidase / Q000737:chemistry; D011485:Protein Binding; D011506:Proteins / Q000187:drug effects* / Q000528:radiation effects*; D011675:Pulse Radiolysis; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D013050:Spectrometry, Fluorescence; D014364:Tryptophan / Q000737:chemistry
chemical_list: D000975:Antioxidants; D004220:Disulfides; D005609:Free Radicals; D011506:Proteins; D011837:Radiation-Protective Agents; D014364:Tryptophan; D009113:Muramidase; D003474:Curcumin