Isolation and characterization of sigma(70)-retaining transcription elongation complexes from Escherichia coli.
sigma(70) subunit is thought to be released from the core RNA polymerase (RNAP) upon the transition from initiation to elongation or shortly afterward. Here, we identify a population of RNAP from E. coli that retains sigma(70) throughout elongation. The relative amount of this population appears to depend on cellular growth and reaches its maximum during the stationary phase. The proportion of sigma(70)-retaining elongation complexes (EC-sigma(70)) is invariant with various promoters or distances from the transcription start site. EC-sigma(70) responds to pauses, intrinsic terminators, and the elongation factor NusA similarly to EC without sigma(70). However, EC-sigma(70) has a substantially higher ability to support multiple rounds of transcription at certain promoters, suggesting its profound role in gene expression and regulation in bacteria.
Bar-Nahum
G
G
Department of Biochemistry, New York University Medical Center, New York, NY 10016, USA.
Nudler
E
E
D001345:Autoradiography; D004269:DNA, Bacterial / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000302:isolation & purification / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D020035:Holoenzymes / Q000235:genetics / Q000378:metabolism; D046911:Macromolecular Substances; D012313:RNA / Q000378:metabolism; D012808:Sigma Factor / Q000302:isolation & purification / Q000378:metabolism*; D014158:Transcription, Genetic
D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; D020035:Holoenzymes; D046911:Macromolecular Substances; D012808:Sigma Factor; D012313:RNA; C081775:RNA polymerase sigma 70; D012321:DNA-Directed RNA Polymerases