Identification of 16alpha,19-dihydroxyandrostenedione in the serum of pregnant women by gas chromatography-mass spectrometry.
16alpha,19-Dihydroxyandrostenedione [16alpha,19-(OH)2 AD], a potential precursor of estriol, was identified in the serum of pregnant women by gas chromatography-mass spectrometry (GC-MS). Preliminary study indicated that the average serum concentration of 16alpha,19-(OH)2 AD in 10 normal pregnant women was 154.2+/-20 pg/ml. In contrast, the serum concentration of 16alpha,19-dihydroxydehydroepiandrosterone was less than the detection limit (30 pg/mI) of the GC-MS method.
Numazawa
M
M
Tohoku Pharmaceutical University, Aobaku, Sendai, Japan. numazawa@tohoku-pharm.ac.jp
Satoh
S
S
Nagaoka
M
M
Honjo
H
H
Osawa
Y
Y
D000328:Adult; D000735:Androstenedione / Q000031:analogs & derivatives / Q000097:blood*; D002138:Calibration; D003687:Dehydroepiandrosterone / Q000031:analogs & derivatives*; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D011247:Pregnancy; D011263:Pregnancy Trimester, Third
C024368:16-hydroxydehydroepiandrosterone sulfate; D000735:Androstenedione; D003687:Dehydroepiandrosterone; C056897:16,19-dihydroxy-4-androsten-3,17-dione