Polymorphic microsatellites developed by cross-species amplifications in common pheasant breeds.
Genetic variability was analysed in two common breeds of pheasant (Phasianus colchicus L. 1758) by means of cross-species amplifications of microsatellite loci: 154 chicken, Gallus gallus and 32 turkey, Meleagris gallopavo, primers were tested for amplification of pheasant DNA. Thirty-six primers (25 specific for chicken and 11 for turkey) amplified pheasant DNA. Fifteen markers yielded specific products and were tested for polymorphism. Eight of them (55%) were polymorphic, with an average polymorphism of two alleles per locus. Specific polymerase chain reaction (PCR) products were sequenced; repeats were found in 11 of the 15 markers, although only two loci showed the same repeat and could be homologous to chicken ones.
Baratti
M
M
Department of Animal Biology and Genetics, University of Florence, Florence, Italy. baratti@fi.cnr.it
Alberti
A
A
Groenen
M
M
Veenendaal
T
T
Fulgheri
F D
FD
D000483:Alleles; D000818:Animals; D001717:Birds / Q000235:genetics*; D002645:Chickens; D004247:DNA / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D014644:Genetic Variation / Q000235:genetics; D018895:Microsatellite Repeats / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA; D014421:Turkey
D004247:DNA