Diagnosis of HAM/TSP based on CSF proviral HTLV-I DNA and HTLV-I antibody index.
The contribution of human T-cell lymphotropic virus (HTLV-I) DNA by PCR in CSF and the intrathecal synthesis of antibodies to HTLV-I by the antibody index (AI) to the diagnosis of HTLV-1-associated myelopathy (HAM)/tropical spastic paraparesis (TSP) were evaluated. Cases of spastic paraparesis compatible with HAM/TSP had increased AI for HTLV-I (60/73) and HTLV-I proviral sequences in CSF (25/27). Among 27 patients with other neurologic diseases, three had increased AI and another three had positive HTLV-I DNA in CSF. Thus, the combination of PCR for proviral DNA and AI for HTLV-I in CSF provides consistent criteria for the diagnosis of HAM/TSP.
Puccioni-Sohler
M
M
Division of Neurology, Federal University of Rio de Janeiro, Hospital Universitário Clementino Fraga Filho, Brazil.
Rios
M
M
Carvalho
S M
SM
Gonçalves
R R
RR
Oliveira
C
C
Correa
R B
RB
Novis
S
S
de Oliveira
M S
MS
Bianco
C
C
D016009:Chi-Square Distribution; D004279:DNA, Viral / Q000134:cerebrospinal fluid*; D015481:HTLV-I Antibodies / Q000134:cerebrospinal fluid*; D015368:Human T-lymphotropic virus 1 / Q000235:genetics*; D006801:Humans; D009103:Multiple Sclerosis / Q000134:cerebrospinal fluid / Q000175:diagnosis; D009422:Nervous System Diseases / Q000134:cerebrospinal fluid / Q000175:diagnosis; D015493:Paraparesis, Tropical Spastic / Q000134:cerebrospinal fluid / Q000175:diagnosis*; D011533:Proviruses / Q000235:genetics*; D018709:Statistics, Nonparametric
D004279:DNA, Viral; D015481:HTLV-I Antibodies