title: Pathogenesis of SIV pneumonia: selective replication of viral genotypes in the lung.
abstract: Lymphocytic interstitial pneumonia of HIV-infected individuals and SIV pneumonia of macaques are both characterized by diffuse infiltration of the lungs with lymphocytes, plasma cells, and macrophages. This study was undertaken to determine whether there are specific, macrophage-tropic genotypes that selectively replicate in the lung of macaques with SIV pneumonia, as in SIV encephalitis. Using a rapid, reproducible SIV/macaque model of AIDS, 11 pig-tailed macaques were intravenously inoculated with an immunosuppressive viral strain, SIV/DeltaB670, and a macrophage-tropic molecule clone, SIV/17E-Fr, and euthanized at 3 months postinoculation. All 11 macaques had severe (6 macaques) or moderate (5 macaques) pneumonia. To identify the viral genotypes that were replicating in the lung parenchyma, bronchoalveolar lavage (BAL) cells, and peripheral blood mononuclear cells (PBMC) of each macaque, RNA was isolated and the SIV env V1 region was amplified, cloned, and sequenced. Lung homogenates and BAL cells contained a more limited repertoire of viral genotypes than PBMC. SIV/17E-Fr was the major genotype in the lungs of 5 macaques and in BAL cells of 6 macaques. The remainder of the macaques had SIV/17E-Fr and the macrophage-tropic strains of SIV/DeltaB670 clones 2 and 12. In contrast, SIV/17E-Fr was the predominant strain in the PBMC of only 3 of 11 macaques. The viral strain that predominated in PBMC was rarely the strain that predominated in the lungs (only 3 of 11 macaques). The severity of pulmonary lesions did not correlate with the levels of viral RNA in lung homogenates or in plasma. However, when only SIV/17E-Fr was expressed in the lung, the viral load in the lung was significantly higher (P = 0.016) than when SIV/DeltaB670 was present alone or in combination with SIV/17E-Fr. These data suggest that SIV pneumonia is associated with selective replication of specific macrophage-tropic genotypes in the lung and that SIV/17E-Fr has a selective advantage for replication in the lung.
authors.affiliation: Division of Comparative Medicine, Johns Hopkins School of Medicine, Baltimore, Maryland 21287, USA.
authors.forename: T
authors.initials: T
authors.lastname: Babas
authors.forename: E
authors.initials: E
authors.lastname: Vieler
authors.forename: D A
authors.initials: DA
authors.lastname: Hauer
authors.forename: R J
authors.initials: RJ
authors.lastname: Adams
authors.forename: P M
authors.initials: PM
authors.lastname: Tarwater
authors.forename: K
authors.initials: K
authors.lastname: Fox
authors.forename: J E
authors.initials: JE
authors.lastname: Clements
authors.forename: M C
authors.initials: MC
authors.lastname: Zink
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D005838:Genotype; D008171:Lung Diseases / Q000473:pathology / Q000821:virology*; D008214:Lymphocytes / Q000821:virology; D008251:Macaca; D008264:Macrophages / Q000821:virology; D008969:Molecular Sequence Data; D011024:Pneumonia, Viral / Q000276:immunology / Q000473:pathology / Q000821:virology*; D012367:RNA, Viral / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D015302:Simian Immunodeficiency Virus / Q000235:genetics / Q000472:pathogenicity* / Q000502:physiology; D019562:Viral Load; D014779:Virus Replication
chemical_list: D012367:RNA, Viral