Nanowire nanosensors for highly sensitive and selective detection of biological and chemical species.
Boron-doped silicon nanowires (SiNWs) were used to create highly sensitive, real-time electrically based sensors for biological and chemical species. Amine- and oxide-functionalized SiNWs exhibit pH-dependent conductance that was linear over a large dynamic range and could be understood in terms of the change in surface charge during protonation and deprotonation. Biotin-modified SiNWs were used to detect streptavidin down to at least a picomolar concentration range. In addition, antigen-functionalized SiNWs show reversible antibody binding and concentration-dependent detection in real time. Lastly, detection of the reversible binding of the metabolic indicator Ca2+ was demonstrated. The small size and capability of these semiconductor nanowires for sensitive, label-free, real-time detection of a wide range of chemical and biological species could be exploited in array-based screening and in vivo diagnostics.
Cui
Y
Y
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA.
Wei
Q
Q
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA.
Park
H
H
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA.
Lieber
C M
CM
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA.|Division of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138 USA.
D000911:Antibodies, Monoclonal / Q000276:immunology / Q000378:metabolism; D015374:Biosensing Techniques; D001710:Biotin / Q000276:immunology / Q000378:metabolism; D001895:Boron; D002118:Calcium / Q000032:analysis* / Q000378:metabolism; D002147:Calmodulin / Q000378:metabolism; D002623:Chemistry Techniques, Analytical / Q000295:instrumentation* / Q000379:methods; D004553:Electric Conductivity; D006863:Hydrogen-Ion Concentration; D008024:Ligands; D008904:Miniaturization; D011506:Proteins / Q000032:analysis*; D011522:Protons; D012666:Semiconductors; D012680:Sensitivity and Specificity; D012825:Silicon; D019809:Streptavidin / Q000032:analysis / Q000378:metabolism; D013499:Surface Properties
D000911:Antibodies, Monoclonal; D002147:Calmodulin; D008024:Ligands; D011506:Proteins; D011522:Protons; D001710:Biotin; D019809:Streptavidin; D001895:Boron; D002118:Calcium; D012825:Silicon