Choriocarcinoma cells modulate the cytokine production of decidual large granular lymphocytes in coculture.
OBJECTIVE
How do major histocompatibility complex (MHC) I positive/negative choriocarcinoma (CC) cells affect the production of tumor necrosis factor-alpha (TNF-alpha), interferon-gamma (IFN-gamma), interleukin-10 (IL-10), interleukin-4 (IL-4), and granulocyte-macrophage colony-stimulating factor (GM-CSF) by decidual CD56++ cells (large granular lymphocytes [LGL]), LGL-depleted decidual cells (WASH) and unseparated decidual cells (DEC) in cocultures?


METHODS
Decidual tissue was obtained by legal abortions. CD 56++ LGL were isolated in a magnetic cell separator. Cytokines were measured by ELISA. Differences were analyzed for significance by Wilcoxons test.


RESULTS
We found a significant increase of IL-10 in LGL/JEG-3 and of TNF-alpha in LGL/JAR cocultures compared to noncocultured decidual cells. There was a significant increase of IL-10 and TNF-alpha and a significant decrease of GM-CSF in WASH and DEC cocultures with both JEG-3 and JAR. IFN-gamma was only found in 3/11 cases of LGL/JAR cocultures. No IL-4 was found in any experiment.


CONCLUSIONS
MHC class I positive/negative CC modulate the cytokine production of decidual LGL in different ways.
Rieger
L
L
Department of Obstetrics and Gynaecology, The University of Wuerzburg, Germany.
Kämmerer
U
U
Hofmann
J
J
Sütterlin
M
M
Dietl
J
J
D002458:Cell Fractionation; D002478:Cells, Cultured; D002822:Choriocarcinoma / Q000276:immunology; D018920:Coculture Techniques / Q000379:methods; D003470:Culture Media; D016207:Cytokines / Q000096:biosynthesis*; D003656:Decidua / Q000166:cytology / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D015395:Histocompatibility Antigens Class I / Q000276:immunology*; D006801:Humans; D008214:Lymphocytes / Q000276:immunology; D011247:Pregnancy; D014327:Trophoblasts / Q000166:cytology / Q000276:immunology*; D014407:Tumor Cells, Cultured
D003470:Culture Media; D016207:Cytokines; D015395:Histocompatibility Antigens Class I