Capillary electrophoresis of RNA in dilute and semidilute polymer solutions.
We report separations of RNA molecules (281-6583 nucleotides) by capillary electrophoresis in dilute and semidilute solutions of aqueous hydroxyethylcellulose (HEC) ether in varying buffers. RNA mobility and peak band widths are examined under both nondenaturing and also denaturing conditions. From studies of sieving polymer concentration and chain length, it is found that good separations can be obtained in semidilute solutions as well as in dilute solutions. The dependence of RNA mobility on its chain length is consistent with separation by a similar to transient entanglement mechanism in dilute solutions. In semidilute entangled solutions the separation proceeds by segmental motion.
Todorov
T I
TI
Department of Chemistry, University of Michigan, Ann Arbor 48109-1055, USA.
de Carmejane
O
O
Walter
N G
NG
Morris
M D
MD
D001704:Biopolymers; D002482:Cellulose / Q000031:analogs & derivatives* / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D008970:Molecular Weight; D009038:Motion; D009691:Nucleic Acid Denaturation; D009994:Osmolar Concentration; D012313:RNA / Q000032:analysis* / Q000187:drug effects; D014508:Urea / Q000494:pharmacology; D014783:Viscosity
D001704:Biopolymers; D012313:RNA; D014508:Urea; D002482:Cellulose; C002283:hydroxyethylcellulose