Upregulation of the cochaperone Mdg1 in endothelial cells is induced by stress and during in vitro angiogenesis.
Angiogenesis research has focused on receptors and ligands mediating endothelial cell proliferation and migration. Little is known about the molecular mechanisms that are involved in converting endothelial cells from a proliferative to a differentiated state. Microvascular differentiation gene 1 (Mdg1) has been isolated from differentiating microvascular endothelial cells that had been cultured in collagen type I gels (3D culture). In adult human tissue Mdg1 is expressed in endothelial and epithelial cells. Sequence analysis of the full-length cDNA revealed that the N-terminal region of the putative Mdg1-protein exhibits a high sequence similarity to the J-domain of Hsp40 chaperones. We show that this region functions as a bona fide J-domain as it can replace the J-domain of Escherichia coli DnaJ-protein. Mdg1 is also upregulated in primary endothelial and mesangial cells when subjected to various stress stimuli. GFP-Mdg1 fusion constructs showed the Mdg1-protein to be localized within the cytoplasm under control conditions. Stress induces the translocation of Mdg1 into the nucleus, where it accumulates in nucleoli. Costaining with Hdj1, Hdj2, Hsp70, and Hsc70 revealed that Mdg1 colocalizes with Hsp70 and Hdj1 in control and stressed HeLa cells. These data suggest that Mdg1 is involved in the control of cell cycle arrest taking place during terminal cell differentiation and under stress conditions.
Pröls
F
F
Institute of Anatomy II, Albert-Ludwigs-University, Freiburg, 79104, Germany. proels@uni-freiburg.de
Mayer
M P
MP
Renner
O
O
Czarnecki
P G
PG
Ast
M
M
Gässler
C
C
Wilting
J
J
Kurz
H
H
Christ
B
B
D000595:Amino Acid Sequence / Q000502:physiology; D001483:Base Sequence / Q000502:physiology; D002352:Carrier Proteins / Q000378:metabolism; D002451:Cell Compartmentation / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology*; D002455:Cell Division / Q000502:physiology; D002466:Cell Nucleolus / Q000378:metabolism; D002478:Cells, Cultured / Q000378:metabolism; D018076:DNA, Complementary / Q000378:metabolism; D004730:Endothelium, Vascular / Q000378:metabolism / Q000503:physiopathology*; D029968:Escherichia coli Proteins; D005786:Gene Expression Regulation / Q000502:physiology; D050883:HSC70 Heat-Shock Proteins; D050956:HSP40 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D007150:Immunohistochemistry; D008565:Membrane Proteins; D018832:Molecular Chaperones / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D018919:Neovascularization, Physiologic / Q000502:physiology*; D017434:Protein Structure, Tertiary / Q000502:physiology; D012333:RNA, Messenger / Q000378:metabolism; D015854:Up-Regulation / Q000235:genetics*; D014945:Wound Healing / Q000502:physiology
D002352:Carrier Proteins; D018076:DNA, Complementary; C107481:DNAJA1 protein, human; C478021:DNAJB9 protein, human; C494635:DnaJ protein, E coli; D029968:Escherichia coli Proteins; D050883:HSC70 Heat-Shock Proteins; D050956:HSP40 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D008565:Membrane Proteins; D018832:Molecular Chaperones; D012333:RNA, Messenger