[Primary culture of epithelial secretory cells from venom gland of the common adder Vipera berus].
A primary culture of epithelial secretory cells from the venom gland of Vipera berus was obtained. The cells adhered to collagen 1 and to a mixture of adhesion proteins (Matrigel), proliferated and retained the features of differentiation. Electron microscopy demonstrated the presence of all ultrastructures typical of these cells in vivo, a full complex of intercellular junctions, and cellular membrane polarity. The immunohistochemistry confirmed the capacity of secretory cells to synthesize venom in culture. We have studied the role of carbochole, an agonist of M-cholinoreceptor, in the initiation of the secretory cycle in cells in vitro. We propose that M-cholinoreceptors may play an important role in the initiation of the secretory cycle in vivo.
Golubkov
V S
VS
Institute of Theoretical and Experimental Biophysics RAS, Pushchino. golubkov_vlad@mail.ru
Lezhnev
E I
EI
Bezgina
E N
EN
Moshkov
D A
DA
D000818:Animals; D002217:Carbachol / Q000494:pharmacology; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002455:Cell Division; D016764:Cell Polarity; D004847:Epithelial Cells / Q000166:cytology* / Q000378:metabolism; D005088:Exocrine Glands / Q000166:cytology* / Q000378:metabolism; D007365:Intercellular Junctions; D014757:Viper Venoms / Q000378:metabolism*; D017819:Viperidae
D014757:Viper Venoms; D002217:Carbachol
Pervichnaia kul'tura kletok sekretornogo épiteliia iadovitoĭ zhelezy gadiuki obyknovennoĭ Vipera berus.