title: Drug-resistant Trypanosoma congolense in naturally infected donkeys in north Omo Zone, southern Ethiopia.
abstract: A three-part study was conducted to determine the efficacy of isometamidium chloride in donkey populations naturally infected with trypanosomes in north Omo Zone, southern Ethiopia. In the first, 373 randomly selected donkeys from four villages were examined for trypanosome infections by the dark ground/phase contrast buffy coat technique (BCT) in November 1999. The trypanosome prevalence was 18.2% (95% confidence interval (CI): 14.4, 22.5) and Trypanosoma congolense was the most common species accounting for 66.2% of the overall infections. In the second part, 40 infected donkeys were selected and treated with a prophylactic dose of 1.0mg/kg of isometamidium chloride and thereafter monitored every 14 days for 90 days. Trypanosomes were detected in eight donkeys within 1 month and in 20 donkeys within 2 months of treatment. About 16% (5/32) of donkeys infected with T. congolense were detected parasitemic 1 month after treatment. In addition, the result also revealed that all relapse/breakthrough infections were due to T. congolense. In the third part of this study mice were infected with two T. congolense field isolates from donkeys that were found to be parasitemic within 1 or 2 months after isometamidium treatment. The mice were treated with ranges of doses of isometamidium chloride or diminazene aceturate and thereafter followed for relapse infection. Isometamidium chloride at doses 0.5-4 mg/kg body weight and diminazene aceturate at doses of 3.5-28 mg/kg body weight failed completely to cure T. congolense infections in any of the mice.
authors.affiliation: Faculty of Veterinary Medicine, Addis Ababa University, P.O. Box 34, Debre Zeit, Ethiopia.
authors.forename: E
authors.initials: E
authors.lastname: Assefa
authors.forename: G
authors.initials: G
authors.lastname: Abebe
mesh_terms: D000818:Animals; D003430:Cross-Sectional Studies; D004133:Diminazene / Q000031:analogs & derivatives / Q000494:pharmacology* / Q000627:therapeutic use; D018432:Drug Resistance, Multiple; D018397:Equidae / Q000469:parasitology*; D005002:Ethiopia / Q000453:epidemiology; D005260:Female; D008297:Male; D051379:Mice; D010617:Phenanthridines / Q000494:pharmacology* / Q000627:therapeutic use; D013997:Time Factors; D016896:Treatment Outcome; D014344:Trypanocidal Agents / Q000494:pharmacology* / Q000627:therapeutic use; D014348:Trypanosoma congolense / Q000187:drug effects*; D014353:Trypanosomiasis, African / Q000188:drug therapy / Q000453:epidemiology / Q000662:veterinary*
chemical_list: D010617:Phenanthridines; D014344:Trypanocidal Agents; C000702:isometamidium chloride; C003915:diminazene aceturate; D004133:Diminazene