title: Reactive oxygen metabolites induce a biphasic contractile response in microvascular lung pericytes.
abstract: BACKGROUND
  The changes in microvascular permeability characteristic of postinjury inflammation and sepsis may involve dysfunctional regulatory mechanisms at the capillary level. Pericytes, positioned abluminal to microvascular endothelium may, by their contractility, contribute to this regulation. Reactive oxygen metabolites (ROMs), well-known participants in lung inflammation, may exert an effect on pericytes, leading to changes in permeability and adult respiratory distress syndrome. This study investigates the effect of ROMs and antioxidants in an established in vitro assay of pericyte contractility.
  
  
  METHODS
  Rat lung pericytes were cultured on collagen gel matrices. After exposure to the ROMs, the surface area of the collagen disks was digitally quantified (an integrated measure of cellular contraction) at 10 and 30 minutes. The cells were exposed to hydrogen peroxide and pyrogallol at 10, 100, and 1,000 micromol/L. Antioxidant effects of catalase (100 micromol/L), superoxide dismutase (100 micromol/L), and pretreatment with vitamin E (1 mmol/L) were quantified.
  
  
  RESULTS
  Hydrogen peroxide and pyrogallol induced concentration-dependent relaxation at 10 minutes. Conversely, concentration-dependent contraction was seen at 30 minutes. Catalase completely attenuated both responses, whereas superoxide dismutase had no effect. Vitamin E had no effect at 10 minutes but partially attenuated the contraction seen at 30 minutes.
  
  
  CONCLUSIONS
  ROMs are capable of producing early relaxation and late contraction in cultured lung pericytes. Whereas catalase attenuates both responses, membrane-bound vitamin E only partially attenuates late contraction. This suggests two separate mechanisms: early physiologic relaxation through signaling pathways affecting actin/myosin tone, and late membrane damage causing contraction. Either pathway may cause dysfunction in pulmonary capillary fluid regulation.
authors.affiliation: Department of Surgery, School of Medicine, Wayne State University, Detroit, Michigan 48201, USA.
authors.forename: S
authors.initials: S
authors.lastname: Kerkar
authors.forename: C
authors.initials: C
authors.lastname: Speyer
authors.forename: J
authors.initials: J
authors.lastname: Tyburski
authors.forename: C
authors.initials: C
authors.lastname: Steffes
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002374:Catalase / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004347:Drug Interactions; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008168:Lung / Q000187:drug effects; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects*; D016877:Oxidants / Q000494:pharmacology*; D020286:Pericytes / Q000187:drug effects*; D011748:Pyrogallol / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012128:Respiratory Distress Syndrome / Q000209:etiology; D014810:Vitamin E / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D016877:Oxidants; D011748:Pyrogallol; D014810:Vitamin E; D006861:Hydrogen Peroxide; D002374:Catalase