Fumonisin B1 metabolism by bovine liver microsomes.
Only limited and contrasting information is available about the metabolic fate in cattle of fumonisin B1, a mycotoxin produced by moulds of Fusarium. This study was carried out to evaluate the hepatic metabolism of fumonisin B1 by bovine liver microsomes. No biodegradation or metabolization of the mycotoxin by liver microsomes was detectable after incubating fumonisin B1 with bovine microsomes in the presence of a regenerating system for 1 h. No aminopolyol 1, aminopolyol 2 or aminopentol, metabolites of fumonisin B1, were detected in any of the incubated samples. The tolerance of ruminants to fumonisin B1 is apparently not dependent on its detoxification in the rumen.
Spotti
M
M
University of Milan, Faculty of Veterinary Medicine, Department of Veterinary Sciences and Technology for Food Safety, Italy. maura.spotti@unimi.it
Pompa
G
G
Caloni
F
F
D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases; D002264:Carboxylic Acids / Q000378:metabolism*; D002274:Carcinogens, Environmental / Q000378:metabolism*; D002417:Cattle / Q000378:metabolism*; D051544:Cytochrome P-450 CYP3A; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D037341:Fumonisins; D006900:Hydroxylation; D008099:Liver / Q000201:enzymology / Q000378:metabolism*; D008862:Microsomes, Liver / Q000201:enzymology / Q000378:metabolism; D010089:Oxidoreductases, N-Demethylating / Q000378:metabolism; D013739:Testosterone / Q000378:metabolism
D002264:Carboxylic Acids; D002274:Carcinogens, Environmental; D037341:Fumonisins; D013739:Testosterone; C056933:fumonisin B1; D003577:Cytochrome P-450 Enzyme System; D001189:Aryl Hydrocarbon Hydroxylases; D051544:Cytochrome P-450 CYP3A; D010089:Oxidoreductases, N-Demethylating