Platelet activation through triathlon competition in ultra-endurance trained athletes: impact of thrombin and plasmin generation and catecholamine release.
The aims of this study were to evaluate whether platelets are activated during strenuous exercise in healthy athletes. Also, to determine the impact of plasmin and thrombin activity and catecholamine release. Previous studies have shown activation of the hemostatic system after competitive exercise, but platelet activation was thought to be absent in trained athletes. The impact of thrombin and other potent platelet activators is still a matter for debate. We examined 30 healthy triathletes during a triathlon competition. Flow cytometric detection of CD62p (P-selectin) was used to measure in vivo activation of platelets. Platelet-leukocyte aggregates were also determined. Thrombin concentration was assessed by the thrombin-antithrombin III complex (TAT) and the fibrinolytic state was characterised by the plasmin-alpha2-antiplasmin complex (PAP). Catecholamines were measured by means of high-pressure liquid chromatography. CD62p rose from baseline (2.3%) to 3.4% and was still elevated after 2 hours (3.1%, p = 0.0133). Platelet-leukocyte aggregates were elevated 30 min after exercise (4.3 % vs 3.6%) and decreased significantly after 60 min (2.9 %, p = 0.008). TAT increased from 3.9 microg/l to 8.3 microg/l after competition and to 5.4 microg/l 2 hours later (p < 0.001). PAP increased 10-fold from 350 microg/l to 3,267 microg/l after the triathlon and was still elevated after 2 hours (1,074 microg/l, p<0.001). No linear correlation was found between the hemostatic markers, catecholamines and platelet activation. Platelets, coagulation and fibrinolysis are activated by competitive exercise in athletes, whereby fibrinolytic changes are pronounced. Mechanisms of platelet activation during exercise include phenomena other than plasmatic hemostatic factors and catecholamines.
Möckel
M
M
Department of Medicine, Humboldt University Berlin, Germany. martin.moeckel@charite.de
Ulrich
N V
NV
Heller
G
G
Röcker
L
L
Hansen
R
R
Riess
H
H
Patscheke
H
H
Störk
T
T
Frei
U
U
Ruf
A
A
D000328:Adult; D002395:Catecholamines / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D015444:Exercise / Q000502:physiology*; D005260:Female; D005341:Fibrinolysin / Q000378:metabolism*; D005342:Fibrinolysis / Q000502:physiology*; D005434:Flow Cytometry; D006801:Humans; D016015:Logistic Models; D008297:Male; D015539:Platelet Activation / Q000502:physiology*; D018709:Statistics, Nonparametric; D013917:Thrombin / Q000378:metabolism*
D002395:Catecholamines; D013917:Thrombin; D005341:Fibrinolysin