[Identification of L-forms of Mycobacterium tuberculosis complex by polymerase chain reaction (PCR)].
Polymerase chain reaction (PCR) was used to verify the tuberculous origin of L-forms isolated from clinical non-respiratory samples from patients with extrapulmonary tuberculosis. PCR was made by using cultured L-forms obtained from negative and positive cultures. PCR used a total of 60 cultured L-forms different in the morphology of colonies and the rate of growth. The total count of L-forms yielding positive amplification with M. tuberculosis complex-specific primers was 51 (85%). L-form passages were subjected to PCR analysis. A total of 14 third-generation L-forms were examined. They turned out to be positive. Thus, the fact that L-forms isolated from nonrespiratory clinical samples from patients with tuberculosis are most commonly L-forms of M. tuberculosis was genetically substantiated.
Vishnevskaia
E B
EB
Bobchenok
A P
AP
Mel'nikova
N N
NN
Vishnevskiĭ
B I
BI
D001431:Bacteriological Techniques; D006801:Humans; D007740:L Forms / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification; D016133:Polymerase Chain Reaction
Identifikatsiia L-form mikobakteriĭ tuberkuleznogo kompleksa s primeneniem polimeraznoĭ tsepnoĭ reaktsii (PTsR).