title: Human reserve pluripotent mesenchymal stem cells are present in the connective tissues of skeletal muscle and dermis derived from fetal, adult, and geriatric donors.
abstract: This study details the profile of 13 cell surface cluster differentiation markers on human reserve stem cells derived from connective tissues. Stem cells were isolated from the connective tissues of dermis and skeletal muscle derived from fetal, mature, and geriatric humans. An insulin/dexamethasone phenotypic bioassay was used to determine the identity of the stem cells from each population. All populations contained lineage-committed myogenic, adipogenic, chondrogenic, and osteogenic progenitor stem cells as well as lineage-uncommitted pluripotent stem cells capable of forming muscle, adipocytes, cartilage, bone, fibroblasts, and endothelial cells. Flow cytometric analysis of adult stem cell populations revealed positive staining for CD34 and CD90 and negative staining for CD3, CD4, CD8, CD11c, CD33, CD36, CD38, CD45, CD117, Glycophorin-A, and HLA DR-II.
authors.affiliation: Division of Basic Medical Science, Mercer University School of Medicine, Macon, Georgia 31207, USA. young_he@mercer.edu
authors.forename: H E
authors.initials: HE
authors.lastname: Young
authors.forename: T A
authors.initials: TA
authors.lastname: Steele
authors.forename: R A
authors.initials: RA
authors.lastname: Bray
authors.forename: J
authors.initials: J
authors.lastname: Hudson
authors.forename: J A
authors.initials: JA
authors.lastname: Floyd
authors.forename: K
authors.initials: K
authors.lastname: Hawkins
authors.forename: K
authors.initials: K
authors.lastname: Thomas
authors.forename: T
authors.initials: T
authors.lastname: Austin
authors.forename: C
authors.initials: C
authors.lastname: Edwards
authors.forename: J
authors.initials: J
authors.lastname: Cuzzourt
authors.forename: M
authors.initials: M
authors.lastname: Duenzl
authors.forename: P A
authors.initials: PA
authors.lastname: Lucas
authors.forename: A C
authors.initials: AC
authors.lastname: Black
mesh_terms: D000368:Aged; D003239:Connective Tissue Cells / Q000166:cytology*; D005260:Female; D005333:Fetus; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D008648:Mesoderm / Q000166:cytology*; D018482:Muscle, Skeletal; D011247:Pregnancy; D012867:Skin; D013234:Stem Cells / Q000166:cytology* / Q000502:physiology*; D014019:Tissue Donors