Production of manganese peroxidase by pellet culture of the lignin-degrading basidiomycete, Pleurotus ostreatus.
Pleurotus ostreatus No. 42 produced the ligninolytic enzymes, manganese peroxidase (MnP) and laccase, in agitation culture in glucose/peptone/wheat-bran medium. Formation of mycelial pellets 1-2 mm in diameter was essential for the production of MnP; and the concentration of dissolved oxygen in the culture medium greatly influenced the production of MnP, a concentration over 5 ppm being necessary for MnP production. The maximal activity of MnP was obtained on days 7-9 of culture, after the consumption of nutrient glucose. Introduction of oxygen from the start of the cultivation caused large pellet formation, which resulted in a low MnP activity level. P. ostreatus No. 42 produced two MnP isozymes in agitation culture. The major isozyme, F-2, was 36.4 kDa and had a pI of 3.95. The MnP characteristics, Km values, dependence on Mn2+ and optimum pH showed the similarity between this isozyme and MnP 3, which was produced under different culture conditions. Analysis of the N-terminal amino acid sequence indicated the close similarity of F-2 to MnP 3.
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Wood Research Institute, Kyoto University, Uji, Japan.
Honda
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Watanabe
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Kuwahara
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D000595:Amino Acid Sequence; D001673:Biodegradation, Environmental; D003470:Culture Media; D005947:Glucose / Q000378:metabolism; D007526:Isoelectric Point; D007527:Isoenzymes / Q000096:biosynthesis / Q000302:isolation & purification; D007700:Kinetics; D042845:Laccase; D008031:Lignin / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D008345:Manganese / Q000494:pharmacology; D008970:Molecular Weight; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000096:biosynthesis; D010100:Oxygen / Q000494:pharmacology; D010544:Peroxidases / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D020076:Pleurotus / Q000201:enzymology* / Q000254:growth & development
D003470:Culture Media; D007527:Isoenzymes; D008345:Manganese; D008031:Lignin; D010088:Oxidoreductases; D042845:Laccase; D010544:Peroxidases; C051129:manganese peroxidase; D005947:Glucose; D010100:Oxygen