title: Immune responses in goats to recombinant hemagglutinin-neuraminidase glycoprotein of Peste des petits ruminants virus: identification of a T cell determinant.
abstract: Peste des petits ruminants virus (PPRV), a member of the genus Morbillivirus within the family Paramyxoviridae, causes a fatal disease 'peste des petits ruminants' in goats and sheep. This enveloped virus is antigenically closely related to rinderpest virus (RPV), which causes a similar but distinct disease in large ruminants. PPRV harbors two major surface glycoproteins, the hemagglutinin-neuraminidase (HN) and the fusion (F) proteins. The surface glycoproteins of morbilliviruses are highly immunogenic and confer protective immunity. In this study, we investigated the immune responses generated in goats immunized with low doses of purified recombinant extracellular baculovirus carrying a membrane bound form of the HN protein of PPRV without any adjuvant. We report that the immunized goats develop both humoral and cell-mediated immune responses. Antibodies generated in the immunized animals could neutralize both PPRV and RPV in vitro. Further, using a combination of Escherichia coli expressed deletion mutants of PPRV-HN and RPV-H proteins, and synthetic peptides corresponding to the highly conserved N-terminal sequences of MV-H protein, we have mapped an N-terminal T cell determinant (amino acids 123-137) and a C-terminal domain (amino acids 242-609) harboring potential T cell determinant(s) in goats.
authors.affiliation: Department of Microbiology and Cell Biology, Indian Institute of Science, 560 012, Bangalore, India.
authors.forename: G
authors.initials: G
authors.lastname: Sinnathamby
authors.forename: G J
authors.initials: GJ
authors.lastname: Renukaradhya
authors.forename: M
authors.initials: M
authors.lastname: Rajasekhar
authors.forename: R
authors.initials: R
authors.lastname: Nayak
authors.forename: M S
authors.initials: MS
authors.lastname: Shaila
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis / Q000276:immunology; D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology*; D003429:Cross Reactions; D018076:DNA, Complementary / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli; D006023:Glycoproteins / Q000235:genetics / Q000276:immunology; D015511:Goat Diseases / Q000276:immunology / Q000517:prevention & control*; D006041:Goats / Q000276:immunology*; D015707:HN Protein / Q000235:genetics / Q000276:immunology*; D007111:Immunity, Cellular; D008213:Lymphocyte Activation; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D029021:Peste-des-Petits-Ruminants / Q000276:immunology / Q000517:prevention & control*; D018107:Peste-des-petits-ruminants virus / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology; D012302:Rinderpest virus / Q000276:immunology; D017384:Sequence Deletion; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D014611:Vaccination / Q000662:veterinary*; D014760:Viral Fusion Proteins / Q000235:genetics / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D018076:DNA, Complementary; C096524:F protein, Peste-des-petits-ruminants virus; C079748:F protein, Rinderpest virus; D006023:Glycoproteins; D015707:HN Protein; D008565:Membrane Proteins; D011994:Recombinant Proteins; D014760:Viral Fusion Proteins