Expression of clpX, an ATPase subunit of the Clp protease, is heat and cold shock inducible in Lactococcus lactis.
In this study, the clpX gene and surrounding sequences were cloned and sequenced from Lactococcus lactis. The putative clpX gene encodes a 411 amino acid polypeptide with a predicted molecular weight of 45.8 kDa. Analysis of the relative levels of clpX transcript revealed that in addition to a role in proteolysis of heat damaged proteins, ClpX may also be involved in cryoprotection.
Skinner
M M
MM
Department of Microbiology, Oregon State University, Corvallis 97331-3804, USA.
Trempy
J E
JE
D000074183:ATPases Associated with Diverse Cellular Activities; D000251:Adenosine Triphosphatases; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D003080:Cold Temperature; D015925:Cryopreservation; D049071:Endopeptidase Clp; D029968:Escherichia coli Proteins; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D005798:Genes, Bacterial / Q000235:genetics; D006360:Heat-Shock Proteins / Q000235:genetics; D006358:Hot Temperature; D013294:Lactococcus lactis / Q000235:genetics*; D018832:Molecular Chaperones; D008969:Molecular Sequence Data; D012697:Serine Endopeptidases
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; D012697:Serine Endopeptidases; D049071:Endopeptidase Clp; D000251:Adenosine Triphosphatases; C088072:ClpX protein, E coli; D000074183:ATPases Associated with Diverse Cellular Activities