Crystal structure of a carbon monoxide dehydrogenase reveals a [Ni-4Fe-5S] cluster.
The homodimeric nickel-containing CO dehydrogenase from the anaerobic bacterium Carboxydothermus hydrogenoformans catalyzes the oxidation of CO to CO2. A crystal structure of the reduced enzyme has been solved at 1.6 angstrom resolution. This structure represents the prototype for Ni-containing CO dehydrogenases from anaerobic bacteria and archaea. It contains five metal clusters of which clusters B, B', and a subunit-bridging, surface-exposed cluster D are cubane-type [4Fe-4S] clusters. The active-site clusters C and C' are novel, asymmetric [Ni-4Fe-5S] clusters. Their integral Ni ion, which is the likely site of CO oxidation, is coordinated by four sulfur ligands with square planar geometry.
Dobbek
H
H
Max-Planck-Institut für Biochemie, Abteilung Strukturforschung, Am Klopferspitz 18a, D-82152 Martinsried, Germany. dobbek@biochem.mpg.de
Svetlitchnyi
V
V
Gremer
L
L
Huber
R
R
Meyer
O
O
D000445:Aldehyde Oxidoreductases / Q000737:chemistry* / Q000378:metabolism*; D001421:Bacteria, Anaerobic / Q000201:enzymology*; D001665:Binding Sites; D002245:Carbon Dioxide / Q000378:metabolism; D002248:Carbon Monoxide / Q000378:metabolism*; D002384:Catalysis; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003460:Crystallization; D018360:Crystallography, X-Ray; D019281:Dimerization; D004579:Electron Transport; D006860:Hydrogen Bonding; D007501:Iron / Q000737:chemistry* / Q000378:metabolism; D008024:Ligands; D008958:Models, Molecular; D009097:Multienzyme Complexes / Q000737:chemistry* / Q000378:metabolism*; D009532:Nickel / Q000737:chemistry* / Q000378:metabolism; D010084:Oxidation-Reduction; D010459:Peptococcaceae / Q000201:enzymology*; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D021122:Protein Subunits; D013455:Sulfur / Q000737:chemistry* / Q000378:metabolism
D008024:Ligands; D009097:Multienzyme Complexes; D021122:Protein Subunits; D002245:Carbon Dioxide; D013455:Sulfur; D009532:Nickel; D002248:Carbon Monoxide; D007501:Iron; D000445:Aldehyde Oxidoreductases; C019823:carbon monoxide dehydrogenase