Differentiation of Fusobacterium necrophorum subspecies from bovine pathological lesions by RAPD-PCR.
Nineteen strains from bovine abscesses identified as Fusobacterium necrophorum by the VPI method were examined by other methods. The API 20A test kit characterized all 19 strains as F. necrophorum. Seven of the strains had haemagglutinating activity and were classified as F. necrophorum subspecies necrophorum, and the remaining, 12 nonhaemagglutinating strains, were classified as F. necrophorum subspecies funduliforme. We used RAPD-PCR with a 10-mer oligonucleotide primer, W1L-2, to confirm this differentiation of the two subspecies. These results suggest that random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) with a suitable primer can be used as a new tool for the differentiation of F. necrophorum subspecies isolated from bovine pathological lesions.
Narongwanichgarn
W
W
Department of Veterinary Microbiology, Faculty of Agriculture, Miyazaki University, 1-1 Gakuen Kibanadai-Nishi, 889-2192, Miyazaki, Japan.
Kawaguchi
E
E
Misawa
N
N
Goto
Y
Y
Haga
T
T
Shinjo
T
T
D000818:Animals; D015373:Bacterial Typing Techniques; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology* / Q000473:pathology; D004269:DNA, Bacterial / Q000032:analysis*; D005675:Fusobacterium necrophorum / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D019105:Random Amplified Polymorphic DNA Technique / Q000662:veterinary
D004269:DNA, Bacterial