Expression of adhesion molecules and fibronectin of activated peritoneal surface with lipopolysaccharide (LPS) analyzed with immuno SEM.
To disclose cell-to-cell interaction associated with the defensive mechanism of the peritoneum, the peritoneum was stimulated with lipopolysaccharide (LPS) and analyzed three-dimensionally, ultrastructurally, and immunohistochemically with immunoSEM (scanning electron microscopy). The activated hepatic peritoneal surface demonstrated numerous microvilli with the adhesion molecules ICAM-1 and VCAM-1. They were restricted to villi and peaked at 1.5 microg/g body weight of LPS. Delicate strands appeared moderately and were interwoven among microvilli with increasing LPS. These strands did not express ICAM-1 or VCAM-1, but fibronectin. Leukocytes began to adhere to the peritoneal surface above die value of LPS (2.5 microg). These results suggest that the peritoneal surface gives a defensive sheet for cell-to-cell interaction through adhesion molecules and fibronectin.
Liang
Y
Y
Department of Anatomy and Organ Technology, Shinshu University, School of Medicine, Graduate School of Medicine, Institute of Organ Transplants, Matsumoto, Japan.
Jyoukura
K
K
Ogiwara
N
N
Sasaki
K
K
D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002450:Cell Communication / Q000187:drug effects; D004847:Epithelial Cells / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure; D005353:Fibronectins / Q000032:analysis*; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis*; D007365:Intercellular Junctions / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure*; D007962:Leukocytes / Q000502:physiology / Q000648:ultrastructure; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008855:Microscopy, Electron, Scanning; D016253:Microscopy, Immunoelectron; D008871:Microvilli / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure*; D010537:Peritoneum / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D019010:Vascular Cell Adhesion Molecule-1 / Q000032:analysis*
D005353:Fibronectins; D008070:Lipopolysaccharides; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1