[Structural-functional changes in root cells under the action of carbonylcyanide 3-chlorphenylhydrazone].
Structural and functional changes in wheat root cells under the action of protonophores-carbonyl cyanide m-chlorophenylhydrazone (CCCP) were studied. After addition of 0.5 mM CCCP we observed K+ ions uptake from the incubation medium, stimulation of O2 uptake which correlated with the occurrence of condensed mitochondria in the cells (1, 4, 6 h of incubation), and an insignificant increase of heat in roots. Taking into account the protonophoric properties of CCCP, we assume what the observed changes may be associated with the activation of a reverse energy dependent transport of H+ ions from the cytoplasm, due partially to H(+)-ATPase function intensification. The addition of diethylstibestrol (DES), a specific inhibitor of plasma membrane H(+)-ATPase, completely eliminated the stimulating effect of CCCP, that confirms the earlier assumption.
Ponomareva
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Institute of Biochemistry and Biophysics, Kazan' Scientific Centre of RAS. ponomareva@sci.ken.ru
Polygalova
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D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology*; D002462:Cell Membrane / Q000201:enzymology; D002463:Cell Membrane Permeability; D018517:Plant Roots / Q000166:cytology* / Q000187:drug effects* / Q000201:enzymology / Q000648:ultrastructure; D006180:Proton-Translocating ATPases / Q000378:metabolism; D011522:Protons; D014908:Triticum / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology / Q000648:ultrastructure
D011522:Protons; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D006180:Proton-Translocating ATPases
Strukturno-funktsional'nye izmeneniia v kletkakh korneĭ pri deĭstvii karboniltsianid 3-khlorfenilgidrazona.