title: Bacterial diversity in soil samples from two uranium waste piles as determined by rep-APD, RISA and 16S rDNA retrieval.
abstract: The bacterial diversity in two uranium waste piles was studied. Total DNA was recovered from a large number of soil samples collected from different sites and depths in the piles using two procedures for direct lysis. Significant differences in the bacterial composition of the samples were revealed by the use of rep-APD, RISA and 16S ARDREA. The 16S rDNA analyses showed that the uranium wastes were dominated by Acidithiobacillusferrooxidans and by several Pseudomonas species classified in the gamma-subdivision of the Proteobacteria. The three kinds of A. ferrooxidans 16S and IGS rDNA specific fragments that were found corresponded to the three phylogenetic groups recognised in this species. This microdiversity probably reflects the genetic adaptation of the uranium waste strains to different concentrations of heavy metals.
authors.affiliation: Institute ofRadiochemistry, Forschungszentrum Rossendorf, Dresden, Germany. s.selenska-pobell@fz-rossendorf.de
authors.forename: S
authors.initials: S
authors.lastname: Selenska-Pobell
authors.forename: G
authors.initials: G
authors.lastname: Kampf
authors.forename: K
authors.initials: K
authors.lastname: Hemming
authors.forename: G
authors.initials: G
authors.lastname: Radeva
authors.forename: G
authors.initials: G
authors.lastname: Satchanska
mesh_terms: D001483:Base Sequence; D016172:DNA Fingerprinting / Q000379:methods*; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D020563:Gammaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D011549:Pseudomonas / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D011850:Radioactive Waste; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D012990:Soil Pollutants, Radioactive; D014501:Uranium
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D011850:Radioactive Waste; D012990:Soil Pollutants, Radioactive; D014501:Uranium