Effects of acute exercise on the gluconeogenic capacity of periportal and perivenous hepatocytes.
The present study was conducted to examine the effect of a single bout of exercise (rodent treadmill, 60 min at 26 m/min, 0% grade) on the gluconeogenic activity of periportal hepatocytes (PP-H) and perivenous hepatocytes (PV-H) in fasted (18 h) rats. Isolated PP-H and PV-H, obtained by selective destruction following liver perfusion with digitonin and collagenase, were incubated with saturating concentrations of alanine (Ala; 20 mM) or a mixture of lactate and pyruvate (Lac+Pyr; 20:2 mM) to determine the glucose production flux (J(glucose)) in the incubation medium. Results show that, in the resting conditions, J(glucose) from all exogenous substrates was significantly higher (P < 0.01) in PP-H than in PV-H. Exercise, compared with rest, resulted in a higher J(glucose) (P < 0.01) from Lac+Pyr substrate in the PV-H but not in the PP-H, resulting in the disappearance of the difference in J(glucose) between PP-H and PV-H. Exercise, compared with rest, led to a higher J(glucose) (P < 0.01) from Ala substrate in both PP-H and PV-H. However, the exercise-induced increase in J(glucose) (gluconeogenic activity) from Ala substrate was higher in PV-H than in PP-H, resulting, as from Lac+Pyr substrate, in the disappearance (P > 0.05) of the difference of J(glucose) between PP-H and PV-H. It is concluded that exercise differentially stimulates the gluconeogenic activity of PV-H to a larger extent than PP-H, indicative of a heterogeneous metabolic response of hepatocytes to exercise.
Désy
F
F
Département de Kinésiologie, Université de Montréal, Montréal, Québec, Canada H3C 3J7.
Burelle
Y
Y
Bélanger
P
P
Gascon-Barré
M
M
Lavoie
J M
JM
D000409:Alanine / Q000493:pharmacokinetics; D000818:Animals; D004734:Energy Metabolism / Q000502:physiology; D005943:Gluconeogenesis / Q000502:physiology*; D005947:Glucose / Q000378:metabolism; D022781:Hepatocytes / Q000378:metabolism*; D007785:Lactose / Q000493:pharmacokinetics; D008099:Liver / Q000098:blood supply / Q000166:cytology / Q000378:metabolism*; D008297:Male; D005082:Physical Exertion / Q000502:physiology*; D011169:Portal Vein / Q000378:metabolism; D019289:Pyruvic Acid / Q000493:pharmacokinetics; D051381:Rats; D017207:Rats, Sprague-Dawley
D019289:Pyruvic Acid; D005947:Glucose; D007785:Lactose; D000409:Alanine