Dietary boron supplementation enhances the effects of estrogen on bone mineral balance in ovariectomized rats.
The present study investigated whether boron would enhance the action of 17beta-estradiol (E2) or parathyroid hormone (PTH) on bone mineral balance in ovariectomized (OVX) rats. Forty-three days after OVX, the rats were treated for 5 wk with vehicle, boron (5 ppm as boric acid), E2 (30 microg/kg/d, sc), PTH (60 microg/kg/d, sc), or a combination of boron and E2 or PTH. Bone mineral balance was assessed by measuring apparent absorption, excretion, and retention of calcium (Ca), phosphorus (P), and magnesium (Mg). Serum Ca, P, Mg, and osteocalcin were also measured in this experiment. Boron alone had no effects on food consumption, weight gain, bone mineral balance, and serum levels of Ca, P, Mg, and osteocalcin. E2 alone increased serum P and Mg and decreased serum osteocalcin, but it had no effect on bone mineral balance. The combination of boron and E2 markedly improved apparent absorption of Ca, P, and Mg. In addition, the combination treatment increased the apparent retention of Ca and Mg (but not P) and also increased serum Ca and Mg but not serum P. On the other hand, boron cotreatment did not prevent the E2-induced reduction in serum osteocalcin in OVX rats. PTH alone significantly increased serum Ca, P, Mg, and osteocalcin concentrations, although it had no effect on bone mineral balance. Contrary to the boron-E2 combination treatment, the combination of boron and PTH did not enhance bone mineral balance. However, inasmuch as boron-PTH cotreatment did not enhance the stimulatory action of PTH on serum Ca, P, and osteocalcin, boron completely abolished the stimulatory effect of PTH on serum Mg. In conclusion, we have demonstrated for the first time that although boron by itself has no effect on bone mineral homeostasis, it appears to have synergistic enhancing effects on the action of E2 on Ca and Mg homeostasis in OVX rats.
Sheng
M H
MH
Department of Human Nutrition, Foods, and Exercise, Virginia Polytechnic Institute and State University, Blacksburg 24060, USA.
Taper
L J
LJ
Veit
H
H
Thomas
E A
EA
Ritchey
S J
SJ
Lau
K H
KH
D000818:Animals; D015519:Bone Density / Q000187:drug effects*; D001895:Boron / Q000008:administration & dosage* / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism / Q000652:urine; D004032:Diet; D004958:Estradiol / Q000097:blood / Q000502:physiology*; D005247:Feeding Behavior; D005260:Female; D008274:Magnesium / Q000378:metabolism / Q000652:urine; D015675:Osteocalcin / Q000097:blood; D010052:Ovariectomy; D010281:Parathyroid Hormone / Q000494:pharmacology; D010758:Phosphorus / Q000378:metabolism / Q000652:urine; D051381:Rats; D017207:Rats, Sprague-Dawley; D015430:Weight Gain
D010281:Parathyroid Hormone; D015675:Osteocalcin; D010758:Phosphorus; D004958:Estradiol; D008274:Magnesium; D001895:Boron; D002118:Calcium