title: Sensitive LC determination of piroxicam after in vitro transdermal permeation studies.
abstract: A direct, very sensitive, simple and rapid high-performance liquid chromatographic (HPLC) method for the determination of piroxicam, with tenoxicam as internal standard, has been developed and validated. Samples were chromatographed on a 5 microm Scharlau C(18) column. The mobile phase was a mixture of acetonitrile-acetic acid 4% (pH 2.8) (45:55, v/v). Detection was at 354 nm and the run time was 7 min. The limit of detection was 0.025 microg/ml. The detector response was found to be linear in the concentration range 0.05-9 microg/ml. This HPLC assay has been applied to measure the 'in vitro' percutaneous permeation of piroxicam through abdominal hairless rat skin, using Franz-type diffusion cells, in order to obtain the concentration-time profiles of piroxicam.
authors.affiliation: Centro Galénico, Departamento de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia, Universidad de Navarra, 31080 Pamplona, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Doliwa
authors.forename: S
authors.initials: S
authors.lastname: Santoyo
authors.forename: M A
authors.initials: MA
authors.lastname: Campanero
authors.forename: P
authors.initials: P
authors.lastname: Ygartua
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000493:pharmacokinetics*; D002851:Chromatography, High Pressure Liquid; D010894:Piroxicam / Q000031:analogs & derivatives* / Q000493:pharmacokinetics*; D051381:Rats; D015203:Reproducibility of Results; D012869:Skin Absorption; D013329:Structure-Activity Relationship
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D010894:Piroxicam; C032801:tenoxicam