Highly efficient expression of rabbit neutrophil peptide-1 gene in Chlorella ellipsoidea cells.
A highly efficient system was developed for the expression of foreign genes in Chlorella ellipsoidea cells. The effect of five promoters on the expression efficiency of beta-glucuronidase (GUS) gene was evaluated by transient expression of the UidA gene. Among these promoters, Ubiquitin-omega was found to be the most efficient and was selected to drive the expression of foreign genes in Chlorella cells. A gene encoding the mature rabbit neutrophil peptide-1 (NP-1) was introduced into the cells. Integration of the gene for NP-1 into the Chlorella genome was confirmed by PCR and Southern blot analysis. In, vitro anti-microbial testes demonstrated the expression of biologically active NP-1 by the transgenic Chlorella cells.
Chen
Y
Y
Group 603, Institute of Genetics, Chinese Academy of Sciences, Beijing, PR China.
Wang
Y
Y
Sun
Y
Y
Zhang
L
L
Li
W
W
D000818:Animals; D000890:Anti-Infective Agents / Q000378:metabolism; D015139:Blotting, Southern; D002708:Chlorella / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D005786:Gene Expression Regulation; D005966:Glucuronidase / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011817:Rabbits / Q000235:genetics*; D023084:alpha-Defensins / Q000235:genetics*
D000890:Anti-Infective Agents; C446100:NP-1 protein, Oryctolagus cuniculus; D023084:alpha-Defensins; D004247:DNA; D005966:Glucuronidase