Identification of a H+/glucose and galactose symporter gene glt from Xanthomonas oryzae pv. oryzae.
We identified a glucose and galactose transporter gene from the plant-pathogenic bacterium Xanthomonas oryzae pv. oryzae. Sequence analysis indicated that the gene, named glt, encoded a polypeptide of 592 amino acid residues and the product was significantly homologous with members of the Na+/glucose cotransporter (SGLT) family from mammalian and bacterial origin, especially with vSGLT from Vibrio parahaemolyticus (50% identity). GLT functioned as a glucose and galactose transporter in an Escherichia coli mutant deficient in glucose and galactose transport activity. A protonophore inhibited the transport activity, suggesting that GLT is a H+-coupled glucose/galactose symporter.
Tsuge
S
S
Laboratory of Plant Pathology, Faculty of Agriculture, Kyoto Prefectural University, Kyoto, Japan. s_tsuge@love.kpu.ac.jp
Furutani
A
A
Kubo
Y
Y
Horino
O
O
D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D001483:Base Sequence; D005690:Galactose / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009004:Monosaccharide Transport Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D012275:Oryza / Q000382:microbiology*; D010935:Plant Diseases / Q000382:microbiology; D027981:Symporters; D014974:Xanthomonas / Q000235:genetics* / Q000378:metabolism
D001426:Bacterial Proteins; D008565:Membrane Proteins; D009004:Monosaccharide Transport Proteins; D027981:Symporters; C445964:glt protein, Xanthomonas oryzae; C089820:monosaccharide-hydrogen cotransporter; D005947:Glucose; D005690:Galactose