Identification of mucosal injury in the murine nasal airways by magnetic resonance imaging: site-specific lesions induced by 3-methylindole.
A magnetic resonance imaging (MRI) technique was developed to identify mucosal damage to the nasal passages of mice resulting from exposure to respiratory toxicants. 3-Methylindole (3-MI) was chosen as a model nasal toxicant because systemic administration of this compound in mice results in a well-characterized necrotizing nasal lesion that is restricted to the olfactory mucosa. MRI technology allows imaging of the same mice before and at time points after injection. In addition, morphological alterations and increases in the area of sinus cavity airspace can be followed as a function of dose and time following exposure. For 3-MI, the cross-sectional area of the sinus airspaces increased by 1.7-fold in mice injected with 200 mg/kg and 2.6-fold in mice injected with 300 mg/kg at 3 days after injection. Alterations in the nasal turbinates lined by olfactory mucosa were identified 1, 3, and 6 days postadministration of 3-MI using MRI. Postmortem histological examination of the nasal tissue confirmed the intranasal location and distribution of the 3-MI-induced lesions observed by MRI. MRI can be a useful technique to identify toxicant-induced mucosal injury in the nasal passages at an in-plane resolution less than 60 microm.
Wiethoff
A J
AJ
Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213, USA.
Harkema
J R
JR
Koretsky
A P
AP
Brown
W E
WE
D000818:Animals; D008279:Magnetic Resonance Imaging; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009296:Nasal Cavity / Q000187:drug effects* / Q000473:pathology; D009297:Nasal Mucosa / Q000293:injuries*; D012862:Skatole / Q000633:toxicity*
D012862:Skatole