title: Experimental treatment of vascular graft infection due to Staphylococcus epidermidis by in situ replacement with a rifampin-bonded polyester graft.
abstract: In situ prosthetic graft replacement (ISPGR) of an infected prosthesis raises the risk of recurrent infection in the new graft, especially in cases involving drug-resistant microorganisms. The purpose of this animal study was to evaluate in situ replacement of a vascular graft infected by a highly rifampin-resistant strain of Staphylococcus epidermidis with the use of a rifampin-bonded polyester graft. Antibiotic bonding was obtained by soaking grafts in a high dose of rifampin solution (60 mg/mL). The infrarenal abdominal aorta of 20 dogs was replaced using a polyester prosthesis infected with a highly rifampin-resistant strain of Staphylococcus epidermidis. One week later, the 18 surviving animals were randomized into three groups. Group I (n = 6) did not undergo reoperation. Group II (n = 6) underwent ISPGR using a rifampin-bonded prosthesis. Group III (n = 6) underwent ISPGR using an untreated prosthesis. All surviving animals were killed 28 days after the first procedure. Infectious signs were noted and bacteriological study was carried out on explanted prostheses and various tissue samples. The findings of this experimental study show that soaking a polyester prosthesis in a high-dose rifampin solution can prevent reinfection after in situ replacement of a prosthesis infected by a highly rifampin-resistant Staphylococcus epidermidis.
authors.affiliation: Service de Chirurgie Vasculaire, Hôpital Ambroisé Paré, Boulogne-Billancourt, France.
authors.forename: M
authors.initials: M
authors.lastname: Coggia
authors.forename: O
authors.initials: O
authors.lastname: Goëau-Brissonnière
authors.forename: V
authors.initials: V
authors.lastname: Leflon
authors.forename: M H
authors.initials: MH
authors.lastname: Nicolas
authors.forename: J C
authors.initials: JC
authors.lastname: Pechère
mesh_terms: D000714:Anastomosis, Surgical / Q000009:adverse effects / Q000401:mortality; D000818:Animals; D000904:Antibiotics, Antitubercular / Q000627:therapeutic use*; D004195:Disease Models, Animal; D004285:Dogs; D005260:Female; D011091:Polyesters / Q000627:therapeutic use*; D019919:Prosthesis Implantation / Q000009:adverse effects* / Q000401:mortality; D011897:Random Allocation; D012293:Rifampin / Q000627:therapeutic use*; D013203:Staphylococcal Infections / Q000150:complications* / Q000401:mortality / Q000628:therapy*; D013212:Staphylococcus epidermidis; D013530:Surgical Wound Infection / Q000209:etiology* / Q000401:mortality / Q000628:therapy*; D016019:Survival Analysis; D013997:Time Factors; D016896:Treatment Outcome; D014656:Vascular Surgical Procedures
chemical_list: D000904:Antibiotics, Antitubercular; D011091:Polyesters; D012293:Rifampin