title: Improved synchrony of estrus and ovulation with the addition of GnRH to a melengestrol acetate-prostaglandin F2alpha synchronization treatment in beef heifers.
abstract: The objective of this experiment was to determine the effect of a GnRH injection within a melengestrol acetate (MGA)-PGF2alpha (PGF) estrus synchronization protocol on follicular dynamics and synchronization of estrus. Pubertal crossbred beef heifers (n = 34) were randomly assigned to one of two treatments. Both treatment groups were fed MGA (0.5 mg x hd(-1) x d(-1)) for 14 d and injected (i.m.) with PGF (25 mg of Lutalyse) 19 d after MGA withdrawal. Melengestrol acetate was delivered in a feed supplement of 1.8 kg x hd(-1) x d(-1). Seventeen heifers received an injection of GnRH (100 microg Cystorelin) 12 d after MGA withdrawal and 7 d before PGF. The control group (n = 17) received only MGA-PGF. Estrus was detected four times/d for 7 d beginning on the day PGF was injected. Transrectal ultrasonography was performed daily on eight heifers from each treatment to monitor ovarian activity and characterize changes in follicular dynamics after MGA withdrawal and until ovulation after PGF. Each of the GnRH-treated heifers either ovulated or had a luteinized dominant follicle following GnRH and subsequently initiated a new follicular wave (8/8, 100%). All GnRH-treated heifers (17/17, 100%) and 94% of controls (16/17) exhibited estrus after PGF. Estrus was exhibited over a 132-h period (12 to 144 h) for control heifers compared with 60 h (48 to 108 h) for GnRH-treated heifers. The peak synchronized period for both treatments was between 48 and 72 h after PGF, during which time 76% (13/17) of the GnRH-treated heifers exhibited estrus compared with 63% (10/16) for controls. Seventy-one percent (12/17) of the GnRH-treated heifers exhibited estrus from 48 to 60 h after PGF, compared with 38% (6/16) for controls (P < 0.05). In summary, injection of GnRH within a 14- to 19-d MGA-PGF protocol increased the synchrony of estrus during the synchronized period and concentrated the period of detected estrus. This protocol may offer potential for the fixed-time insemination of replacement beef heifers.
authors.affiliation: Department of Animal Sciences, University of Missouri, Columbia 65211, USA.
authors.forename: S L
authors.initials: SL
authors.lastname: Wood
authors.forename: M C
authors.initials: MC
authors.lastname: Lucy
authors.forename: M F
authors.initials: MF
authors.lastname: Smith
authors.forename: D J
authors.initials: DJ
authors.lastname: Patterson
mesh_terms: D000822:Animal Husbandry / Q000379:methods*; D000818:Animals; D002417:Cattle / Q000502:physiology*; D015237:Dinoprost / Q000494:pharmacology*; D004338:Drug Combinations; D004973:Estrus Synchronization / Q000187:drug effects*; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000494:pharmacology*; D008552:Melengestrol Acetate / Q000494:pharmacology*; D006080:Ovarian Follicle / Q000187:drug effects / Q000254:growth & development; D010060:Ovulation / Q000187:drug effects*; D011374:Progesterone / Q000097:blood
chemical_list: D004338:Drug Combinations; D007987:Gonadotropin-Releasing Hormone; D011374:Progesterone; D008552:Melengestrol Acetate; D015237:Dinoprost