Detection of B virus infection in cynomolgus monkeys by ELISA using simian agent 8 as alternative antigen.
The use of simian agent 8 (SA8) as an antigen for B virus (BV) antibody detection was evaluated in cynomolgus monkeys. Seventy-two sera judged as positive using BV antigen were all positive when the SA8 antigen was used. Out of 28 BV-negative sera 2 were positive against the SA8 antigen and one serum was classified as indeterminate. The present data indicates that detection of BV antibody can be achieved accurately and safely by enzyme-linked immunosorbent assay (ELISA) using SA8 antigen.
Takano
J
J
Corporation for Production and Research of Laboratory Primates, 1 Hachimandai, Tsukuba-shi, Ibaraki 305-0843, Japan.
Narita
T
T
Fujimoto
K
K
Mukai
R
R
Yamada
A
A
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D000956:Antigens, Viral / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005188:False Negative Reactions; D006564:Herpesviridae / Q000276:immunology; D006566:Herpesviridae Infections / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*; D006568:Herpesvirus 1, Cercopithecine / Q000276:immunology*; D008252:Macaca fascicularis / Q000821:virology*; D012680:Sensitivity and Specificity
D000914:Antibodies, Viral; D000956:Antigens, Viral