title: Dispase facilitates posterior vitreous detachment during vitrectomy in young pigs.
abstract: OBJECTIVE
  To evaluate the role of intravitreal dispase in conjunction with pars plana vitrectomy to facilitate the creation of a posterior vitreous detachment (PVD) in young pig eyes.
  
  
  METHODS
  Twenty-four eyes of 24 animals were randomized to receive an intravitreal injection of dispase (50 microg/0.05 mL) or phosphate buffered saline (PBS) immediately after core vitrectomy and before attempted creation of a posterior cortical vitreous detachment. Following a 15-minute waiting period, surgical creation of a posterior vitreous separation was attempted by aspiration of the posterior vitreous immediately adjacent to the optic disk. Eyes were evaluated postoperatively by clinical examination (1, 4, and 8 weeks) and electroretinography (4 and 8 weeks), after which they were enucleated for light, scanning, and transmission electron microscopy.
  
  
  RESULTS
  Based on intraoperative findings and postoperative scanning electron microscopy, eyes receiving intravitreal dispase exhibited a higher incidence of PVD compared to eyes receiving PBS (P = 0.029). Electroretinographic responses recorded at postoperative weeks 4 and 8 were similar in both dispase and PBS eyes compared to the unoperated fellow eyes. Clinical examinations, including indirect ophthalmoscopy, were indistinguishable between the PBS eyes and 11 of 12 eyes in the dispase group. Light and transmission electron microscopy demonstrated no differences in the retina between the dispase eyes and the PBS operated controls.
  
  
  CONCLUSIONS
  Dispase is a useful adjunct in facilitating surgical creation of a PVDin young pig eyes.
authors.affiliation: Department of Ophthalmology, Duke University School of Medicine, Durham, North Carolina, USA.
authors.forename: L B
authors.initials: LB
authors.lastname: Oliveira
authors.forename: M
authors.initials: M
authors.lastname: Tatebayashi
authors.forename: T H
authors.initials: TH
authors.lastname: Mahmoud
authors.forename: S M
authors.initials: SM
authors.lastname: Blackmon
authors.forename: F
authors.initials: F
authors.lastname: Wong
authors.forename: B W
authors.initials: BW
authors.lastname: McCuen
mesh_terms: D000818:Animals; D004596:Electroretinography; D010450:Endopeptidases / Q000494:pharmacology*; D007267:Injections; D008855:Microscopy, Electron, Scanning; D011897:Random Allocation; D012160:Retina / Q000502:physiology / Q000648:ultrastructure; D013552:Swine; D014821:Vitrectomy / Q000379:methods*; D014822:Vitreous Body / Q000187:drug effects*; D020255:Vitreous Detachment / Q000139:chemically induced*
chemical_list: D010450:Endopeptidases; C023139:dispase