[Study of molecular mechanisms of translation initiation in mammals using in vitro reconstruction of initiation complexes].
Papers on the mechanisms of translation initiation in mammals studied by reconstruction of initiation complexes from individual components are reviewed. The author points to the constraints of this approach and to the pitfalls ignoring which one might come to erroneous conclusions and even artifacts. In addition, some methods employed in the field as well as some technical problems are discussed in the paper, together with the means of obviating them. The review could be a guidebook for newcomers into this quite labor-consuming field.
Shatskiĭ
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shatsky@libro.genebee.msu.su
D000818:Animals; D008322:Mammals; D010442:Peptide Chain Initiation, Translational; D010448:Peptide Initiation Factors / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D012335:RNA, Ribosomal / Q000235:genetics
D010448:Peptide Initiation Factors; D012333:RNA, Messenger; D012335:RNA, Ribosomal
Rekonstruktsiia initsiiruiushchikh kompleksov in vitro dlia issledovaniia molekuliarnykh mekhanizmov initsiatsii transliatsii u mlekopitaiushchikh.