title: IsCT, a novel cytotoxic linear peptide from scorpion Opisthacanthus madagascariensis.
abstract: A novel cytotoxic linear peptide, IsCT, was characterized from scorpion Opisthacanthus madagascariensis. It is a linear peptide with a molecular weight of 1501.9 Da composed of 13 amino acid residues without cysteines. MS/MS analysis showed that its C-terminal is amidated. The identity of IsCT is re-confirmed by comparing the chemical synthesized peptide with the natural one. IsCT demonstrated antimicrobial activity against both gram-positive and gram-negative bacteria and hemolytic activity to sheep red blood cells. Also, it can release histamine from rat peritoneal mast cells. The CD absorption suggested that IsCT had an alpha-helix configuration in aqueous TFE. IsCT is one of the shortest natural cytotoxic peptides described, and it will be a suitable model for studying peptide-lipid interactions.
authors.affiliation: Suntory Institute for Bioorganic Research, Shimamoto, Osaka 618-8503, Japan. dai@sunbor.or.jp
authors.forename: L
authors.initials: L
authors.lastname: Dai
authors.forename: A
authors.initials: A
authors.lastname: Yasuda
authors.forename: H
authors.initials: H
authors.lastname: Naoki
authors.forename: G
authors.initials: G
authors.lastname: Corzo
authors.forename: M
authors.initials: M
authors.lastname: Andriantsiferana
authors.forename: T
authors.initials: T
authors.lastname: Nakajima
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002478:Cells, Cultured; D006461:Hemolysis; D006636:Histamine Release; D008297:Male; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000502:physiology; D017433:Protein Structure, Secondary; D051381:Rats; D017208:Rats, Wistar; D012604:Scorpion Venoms / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D014863:Wasps / Q000737:chemistry
chemical_list: C435485:IsCT protein, Opisthacanthus madagascariensis; D010455:Peptides; D012604:Scorpion Venoms