Distribution of immunophilin FKBP-12 protein and mRNA within the mammalian cochlea and cochlear nucleus.
Immunophilin FK binding protein-12 (FKBP-12), the soluble receptor for the immunosuppressant drug FK506, is involved in a number of neuronal activities including increased nerve regeneration in the peripheral nervous system and enhanced recovery in animal models of neurodegenerative diseases. In addition, FKBP-12 is tightly bound to the calcium release channel ryanodine receptor and physiologically interacts with the inositol 1,4,5-trisphosphate receptor. In nearly all cell types, release of intracellular Ca(2+) and subsequent second messenger signaling involves activation of these ion channels. We determined the distribution of FKBP-12 within the mammalian cochlea and dorsal cochlear nucleus (DCN) in order to gain insight into Ca(2+) regulation within the cochlea and to possibly identify potential cellular targets for neuroimmunophilin ligands that may prove useful in protection and recovery following ototoxic insult. FKBP-12 protein and mRNA were found to be abundant throughout rat and guinea pig cochlea and DCN.
Zajic
G
G
Department of Neuroscience, Amgen Inc, Thousand Oaks, CA 91320-1789, USA. gzajic@amgen.com
Henley
C
C
Louis
J C
JC
Nicolson
M
M
Magal
E
E
D000818:Animals; D015153:Blotting, Western; D003051:Cochlea / Q000378:metabolism*; D017626:Cochlear Nucleus / Q000378:metabolism*; D006168:Guinea Pigs; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D009416:Nerve Regeneration / Q000235:genetics / Q000502:physiology; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism*; D051381:Rats; D019837:Ryanodine Receptor Calcium Release Channel / Q000378:metabolism; D022061:Tacrolimus Binding Protein 1A / Q000235:genetics* / Q000378:metabolism*; D014018:Tissue Distribution
D012333:RNA, Messenger; D019837:Ryanodine Receptor Calcium Release Channel; D022061:Tacrolimus Binding Protein 1A