Nuclear gene transcription and chromatin in Trypanosoma brucei.
As in other eucaryotes, the nuclear genome in Trypanosoma brucei is organised into silent domains and active domains transcribed by distinct RNA polymerases. The basic mechanisms underlying eucaryotic gene transcription are conserved between humans and yeast, and understood in some detail in these cells. Meanwhile, relatively little is known about the transcription machinery, the chromatin templates or their interactions in trypanosomatids. Here, I discuss and compare nuclear gene transcription in T. brucei with transcription in other eucaryotes focusing in particular on mono-allelic transcription of genes that encode the variant surface glycoproteins.
Horn
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London School of Hygiene and Tropical Medicine, Keppel Street, London, WC1E 7HT, UK. david.horn@lshtm.ac.uk
D000595:Amino Acid Sequence; D000818:Animals; D002843:Chromatin / Q000235:genetics*; D012321:DNA-Directed RNA Polymerases / Q000502:physiology; D005786:Gene Expression Regulation; D006657:Histones / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000502:physiology; D014157:Transcription Factors / Q000502:physiology; D014158:Transcription, Genetic; D014346:Trypanosoma brucei brucei / Q000235:genetics*; D014352:Trypanosomiasis / Q000469:parasitology; D014643:Variant Surface Glycoproteins, Trypanosoma / Q000235:genetics*
D002843:Chromatin; D006657:Histones; D014157:Transcription Factors; D014643:Variant Surface Glycoproteins, Trypanosoma; D012321:DNA-Directed RNA Polymerases