title: An electrochemical multienzymatic biosensor for determination of cholesterol.
abstract: This paper describes an electrochemical biosensor for free cholesterol monitoring. The sensor is a multienzymatic electrodic system in which horseradish peroxidase and cholesterol oxidase are simultaneously immobilized within a polymeric film, on the surface of a pyrolitic graphite electrode. From voltammetric and amperometric (flow-injection) data obtained, the efficiency, reproducibility and stability of the system are discussed. Results obtained, of interest for basic and applied biochemistry, represent a first step for construction of a mediator-free biosensor with potentialities for a successful application in the biosensor area.
authors.affiliation: INFM e Dipartimento di Biologia, Università di Roma Tor Vergata, 00133 Rome, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Bongiovanni
authors.forename: T
authors.initials: T
authors.lastname: Ferri
authors.forename: A
authors.initials: A
authors.lastname: Poscia
authors.forename: M
authors.initials: M
authors.lastname: Varalli
authors.forename: R
authors.initials: R
authors.lastname: Santucci
authors.forename: A
authors.initials: A
authors.lastname: Desideri
mesh_terms: D015374:Biosensing Techniques; D002784:Cholesterol / Q000032:analysis* / Q000378:metabolism; D002789:Cholesterol Oxidase; D002942:Circular Dichroism; D004563:Electrochemistry / Q000295:instrumentation*; D004800:Enzymes, Immobilized; D017022:Flow Injection Analysis
chemical_list: D004800:Enzymes, Immobilized; D002784:Cholesterol; D002789:Cholesterol Oxidase