The effect of formulation and concentration of cholesteryl butyrate solid lipid nanospheres (SLN) on NIH-H460 cell proliferation.
Experimental factorial design was used to evaluate the influence of two factors involved in producing cholesteryl butyrate (chol-but) solid lipid nanospheres (SLN), microemulsion formulation and microemulsion/water ratio, on the effect of the SLN on the proliferation of NIH-H460, a non-small-cell lung carcinoma; six experimental settings were tested. The cells were treated with scalar concentrations of cholesteryl butyrate (from 0.008 to 1.000 mM) for each experimental condition; NIH-H460 cell growth was inhibited in all cases. The best experimental setting provided complete inhibition at 0.125 mM chol-but, while at the same concentration sodium butyrate provided only 38% inhibition.
Ugazio
E
E
Dipartimento di Scienza e Tecnologia del Farmaco, Università degli Studi di Torino, Via Giuria 9, 10125 Turin, Italy.
Marengo
E
E
Pellizzaro
C
C
Coradini
D
D
Peira
E
E
Daidone
M G
MG
Gasco
M R
MR
D020148:Butyric Acid / Q000008:administration & dosage* / Q000737:chemistry; D002214:Capsules; D002455:Cell Division / Q000187:drug effects; D002626:Chemistry, Pharmaceutical; D002788:Cholesterol Esters / Q000008:administration & dosage* / Q000737:chemistry; D004655:Emulsions; D006633:Histamine Antagonists / Q000008:administration & dosage* / Q000737:chemistry; D006801:Humans; D008081:Liposomes; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000187:drug effects
D002214:Capsules; D002788:Cholesterol Esters; D004655:Emulsions; D006633:Histamine Antagonists; D008081:Liposomes; D020148:Butyric Acid