Enhancement of phase separation using a drop coalescer in an aqueous two-phase system.
The effect of a drop coalescer on phase separation in a PEG/salt aqueous two-phase system (ATPS) in the absence and presence of protein has been investigated. Raschig rings of ceramic, PTFE and glass were used as a drop coalescer in order to separate the mixture into two phases. Among the three materials PTFE is the most effective in coalescing the dispersed drops, with the throughput with PTFE being twice that without the coalescer. Random packing gives good results for phase separation. Two types of fiber mesh coated with PTFE were also used as drop coalescers, one in a spirally folded form and the other in a three-dimensional lattice-form. Throughput in the PEG/salt system with the three-dimensional lattice-form is 1.2 times as high as that with the spirally folded form. Throughput with the coalescer formed by compiling PTFE Raschig rings and fiber mesh in lattice form is 1.6 and 1.2 times as high as the case of separate use of the fiber mesh and the PTFE Raschig rings, respectively. The hydrophobic surface of PTFE in the compiled coalescer has no significant effect on the recovery fraction of the protein in ATPS.
Ban
T
T
Department of Chemical Engineering, Nagoya University, Japan. h991305d@mbox.media.nagoya-u.ac.jp
Shibata
M
M
Kawaizumi
F
F
Nii
S
S
Takahashi
K
K
D000327:Adsorption; D002853:Chromatography, Liquid / Q000379:methods*; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification
D011506:Proteins