Separation of glyceride positional isomers by silver ion chromatography.
Separation of triglyceride and diglyceride positional isomers by silver ion high-performance liquid chromatography coupled with an evaporative light-scattering detector is described. The triglyceride isomers had a fatty acid composition of CLC and CCL, where C and L were caprylic acid and linoleic acid, respectively. Diglyceride isomers, 1,2(2,3)-diglyceride and 1,3-diglyceride, which contained caprylic acid were separated too. A solvent system based on n-hexane, 2-propanol, ethyl acetate, and acetonitrile with a flow-rate of 0.8 ml/min was developed. Calibration curves of CLC and CCL were achieved with triolein as internal standard. Using this method, the incorporation of linoleic acid onto specific a position of glycerol backbone can be monitored.
Févrie
P
P
Laboratoire Universitaire de Microbiologie Appliquée de Quimper, Université de Bretagne Occidentale, France.
Bine
A
A
Dufossé
L
L
Grée
R
R
Yvergnaux
F
F
D002210:Caprylates / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004075:Diglycerides / Q000302:isolation & purification; D005989:Glycerides / Q000737:chemistry / Q000302:isolation & purification*; D007536:Isomerism; D019787:Linoleic Acid / Q000737:chemistry / Q000302:isolation & purification; D012834:Silver / Q000737:chemistry*
D002210:Caprylates; D004075:Diglycerides; D005989:Glycerides; C058807:1,3-dicapryloylglycerol; D012834:Silver; C057267:1,2-dicaprylin; D019787:Linoleic Acid