title: Radial glia-mediated up-regulation of somatostatin in the regenerating adult fish brain.
abstract: Adult teleost fish, Apteronotus leptorhynchus, exhibit an enormous regenerative capability after application of mechanical lesions to the dorsalmost subdivision of the cerebellum, the corpus cerebelli. Restoration of the neural tissue is achieved by a cascade of processes, including the guidance of migrating new neurons to the site of injury by radial glial fibers. These fibers are characterised by the expression of immunoreactive glial fibrillary acidic protein and by several morphological features. Within 12 h following the lesion, the fraction of radial glial fibers expressing the neuropeptide somatostatin (SRIF) dramatically increased from approximately 1%, as found in the intact brain, to roughly 27% 12-24 h post-lesion. Subsequently, the percentage of SRIF-expressing radial glial fibers gradually declined, until it reached background levels at about 10 days following the injury. We hypothesise that the expression of SRIF is related to the generation and/or differentiation of the new neurons produced in response to the lesion, rather than to the later guidance of these cells along their migratory pathway.
authors.affiliation: School of Biological Sciences, University of Manchester, 3.614 Stopford Building, Oxford Road, Manchester M13 9PT, UK. gunther.zupanc@man.ac.uk
authors.forename: G K
authors.initials: GK
authors.lastname: Zupanc
authors.forename: S C
authors.initials: SC
authors.lastname: Clint
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism* / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D002531:Cerebellum / Q000293:injuries / Q000378:metabolism*; D005260:Female; D005399:Fishes / Q000378:metabolism*; D005904:Glial Fibrillary Acidic Protein / Q000096:biosynthesis; D008297:Male; D009412:Nerve Fibers / Q000378:metabolism; D009457:Neuroglia / Q000378:metabolism*; D012038:Regeneration / Q000502:physiology*; D013004:Somatostatin / Q000378:metabolism*
chemical_list: D005904:Glial Fibrillary Acidic Protein; D013004:Somatostatin