title: Evidence for early local viral replication and local production of antiviral immunity upon mucosal simian-human immunodeficiency virus SHIV(89.6) infection in Macaca nemestrina.
abstract: Transmission of human immunodeficiency virus type 1 (HIV-1) is largely a result of heterosexual exposure, leading many investigators to evaluate mucosal vaccines for protection against intravaginal (i.vag.) transmission in macaque models of AIDS. Relatively little is known, however, about the dynamics of viral replication and the ensuing immune response following mucosal infection. We have utilized a simian-human immunodeficiency virus (SHIV) to study the differences in viremia, CD4 T-cell percentages, and mucosal and systemic anti-SHIV humoral and cellular immune responses during primary infection of animals infected either intravenously (i.v.) or i.vag. Positive viral cocultures, peripheral blood mononuclear cell viral load peaks, and CD4 cell declines were delayed by 1 week in the i.vag. inoculated animals compared to the animals infected i.v., demonstrating delayed viral spreading to the periphery. In contrast, mucosal anti-SHIV antibody levels were greater in magnitude and arose more rapidly and mucosal CD8(+) T-cell responses were enhanced in the i.vag. group animals, whereas both the magnitudes and times of onset of systemic immune responses for the animals in the two groups did not differ. These observations demonstrate that compartmentalization of viral replication and induction of local antiviral immunity occur in the genital tract early after i.vag. but not i.v. inoculation. Induction of mucosal immunity to target this local, contained replication should be a goal in HIV vaccine development.
authors.affiliation: Department of Pathobiology, School of Public Health and Community Medicine, University of Washington, Seattle, Washington 98195, USA.
authors.forename: Z
authors.initials: Z
authors.lastname: Ambrose
authors.forename: K
authors.initials: K
authors.lastname: Larsen
authors.forename: J
authors.initials: J
authors.lastname: Thompson
authors.forename: Y
authors.initials: Y
authors.lastname: Stevens
authors.forename: E
authors.initials: E
authors.lastname: Finn
authors.forename: S L
authors.initials: SL
authors.lastname: Hu
authors.forename: M L
authors.initials: ML
authors.lastname: Bosch
mesh_terms: D000818:Animals; D004279:DNA, Viral / Q000097:blood; D005260:Female; D015497:HIV-1 / Q000235:genetics / Q000276:immunology*; D006801:Humans; D018928:Immunity, Mucosal; D007275:Injections, Intravenous; D018655:Lymphocyte Count; D008254:Macaca nemestrina; D012367:RNA, Viral / Q000097:blood; D015302:Simian Immunodeficiency Virus / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000166:cytology; D013997:Time Factors; D014621:Vagina / Q000821:virology*; D019562:Viral Load; D014779:Virus Replication
chemical_list: D004279:DNA, Viral; D012367:RNA, Viral