Anti-inflammatory and analgesic properties of the stem bark extracts of Erythrophleum suaveolens (Caesalpiniaceae), Guillemin & Perrottet.
The MeOH stem bark extract of Erythrophleum suaveolens dissolved in water and shaken up with ethylacetate (EtOAc) and fractionated on a polyamide column with methanol as eluent produced five principal fractions. These fractions were designated as fraction A (74.8 mg yield and rich in alkaloids), fraction B (36.6 mg), fraction C (7.8 mg yield, monomeric procyanidin), fraction D (26.6 mg yield, rich in monomeric and oligomeric procyanidin), and fraction E (18.1 mg yield, rich in polymeric procyanidin). The original MeOH extract administered (100 mg/kg po) produced about 47% inhibition of carrageenin-induced paw oedema 1 h after administration. Fraction D, obtained from the ethylacetate extract and rich in procyanidins produced over 33% inhibition of carrageenan-induced paw oedema while a dose of 19.2 microg/ml produced 100% inhibitory effect on 5-lipoxygenase. A dose of 100 mg/kg of the MeOH extract also produced over 30% reduction of the sensitivity to pain while 50 mg/kg of fraction D rich in procyanidins produced over 45% analgesic effects. These results were judged significant compared to those obtained with indomethacin and acetylsalicylic acid. These findings suggest that extracts of the bark of Erythrophleum suaveolens possess potent anti-inflammatory and analgesic property and that the procyanidins lead to the observable pharmacological effects.
Dongmo
A B
AB
Faculty of Science, University of Douala, P.O. Box 24157, Douala, Cameroon. adongmo@hotmail.com
Kamanyi
A
A
Anchang
M S
MS
Chungag-Anye Nkeh
B
B
Njamen
D
D
Nguelefack
T B
TB
Nole
T
T
Wagner
H
H
D000698:Analgesia; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000627:therapeutic use*; D002351:Carrageenan / Q000037:antagonists & inhibitors; D005260:Female; D016859:Lipoxygenase Inhibitors; D008297:Male; D008517:Phytotherapy; D010936:Plant Extracts / Q000032:analysis / Q000302:isolation & purification / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar
D000893:Anti-Inflammatory Agents; D016859:Lipoxygenase Inhibitors; D010936:Plant Extracts; D002351:Carrageenan