[Prevalence of major virulence genes among various Vibrio cholerae El-TOR strains for evaluating their epidemiological significance].
Specific oligonucleotide primers were chosen for identifying the fragments of the four major virulence genes of V. cholerae eltor (ctxA, tcpA, toxR, and hap) using the polymerase chain reaction (PCR). In order to estimate the efficiency of complex PCR testing of V. cholerae for evaluation of their epidemiological significance, a collection of 80 V. cholerae eltor strains with known virulence was selected, whose most important specific features had been studied previously. The hap was appropriate species-specific gene making it possible to detect V. cholerae strains regardless of their virulence. The most complete and objective data for evaluating the epidemic significance can be obtained by detecting the presence of three virulence genes (ctxA, tcpA, and toxR) in their chromosome. The prevalence of the above four genes in various V. cholerae strains isolated from the environment during epidemic and non-epidemic periods was studied.
Smirnova
N I
NI
Mikrob Plague Control Institute, Saratov.
Kirillina
O A
OA
Cheldyshova
N B
NB
Kutyrev
V V
VV
D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D001426:Bacterial Proteins; D004268:DNA-Binding Proteins / Q000235:genetics; D036781:Fimbriae Proteins; D005798:Genes, Bacterial; D010641:Phenotype; D016133:Polymerase Chain Reaction; D012697:Serine Endopeptidases; D014157:Transcription Factors / Q000235:genetics; D014734:Vibrio cholerae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D014774:Virulence
D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C085807:TcpA protein, Vibrio cholerae; D014157:Transcription Factors; C499324:toxR protein, Vibrio cholerae; C066719:toxR protein, bacteria; D036781:Fimbriae Proteins; D012697:Serine Endopeptidases
Izuchenie rasprostrannennosti osnovnykh genov virulentnosti sredi razlichnykh shtammov Vibrio cholerae El-TOR dlia opredeleniia ikh épidemicheskoĭ znachimosti.