Analysis of the mammalian talin2 gene TLN2.
We have utilised genomic and EST databases to assemble the sequence of the human talin2 (TLN2) gene. Talin2 protein is similar in size and sequence to talin1 throughout its length (74% identity, 86% similarity). The major differences are in (i) the size of the genes, the TLN2 gene is >200 kb compared with approximately 30 kb for TLN1 due to a difference in intron size, although intron/exon boundaries, with the exception of two, are strictly conserved; (ii) the expression patterns, TLN1 gives rise to an approximately 8-kb mRNA which is observed in all tissues, whereas TLN2 gives rise to multiple transcripts with the highest levels in heart.
Monkley
S J
SJ
Department of Biochemistry, University of Leicester, University Road, Leicester, LE1 7RH, United Kingdom.
Pritchard
C A
CA
Critchley
D R
DR
D020413:3' Untranslated Regions; D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D002874:Chromosome Mapping; D002884:Chromosomes, Human, Pair 15; D002899:Chromosomes, Human, Pair 9; D017124:Conserved Sequence; D003598:Cytoskeletal Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics*; D005091:Exons; D020224:Expressed Sequence Tags; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D009206:Myocardium / Q000378:metabolism; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000378:metabolism; D016608:Talin / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics*; D014018:Tissue Distribution
D020413:3' Untranslated Regions; D003598:Cytoskeletal Proteins; D012333:RNA, Messenger; C436513:TLN2 protein, human; D016608:Talin