title: [Determination of 4-O-methylpyridoxine (MPN) by HPLC with diode-array detector].
abstract: We have developed a simple and rapid method for measuring 4-O-methylpyridoxine (MPN) in the serum of an acute poisoning patient by high-performance liquid chromatograph (HPLC) with a diode-array detector. MPN, a poisonous ingredient of ginkgo seeds was extracted using an Isolute C18 solid-phase extraction column. Equal volumes of the extract were injected into a C18 HPLC column using acetonitrile-10 mM phosphate buffer (pH 3) as the mobile phase. Excellent linearity was obtained over the range of 1-1000 ng at a wavelength of 290 nm, and its detection limit of approximately 0.5 ng was considered to be satisfactory. The mean recovery of MPN in the serum was 92.9 +/- 6.1%. The method was used successfully in a case of acute poisoning.
authors.affiliation: Department of Analytical Chemistry, Niigata College of Pharmacy, Niigata.
authors.forename: M
authors.initials: M
authors.lastname: Fujisawa
authors.forename: M
authors.initials: M
authors.lastname: Nakajima
authors.forename: Y
authors.initials: Y
authors.lastname: Hori
authors.forename: K
authors.initials: K
authors.lastname: Shimada
authors.forename: H
authors.initials: H
authors.lastname: Yoshikawa
authors.forename: K
authors.initials: K
authors.lastname: Wada
authors.forename: M
authors.initials: M
authors.lastname: Haga
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D011736:Pyridoxine / Q000031:analogs & derivatives / Q000302:isolation & purification*
chemical_list: C001737:4-methoxymethylpyridoxine; D011736:Pyridoxine