Expression of phospholipase C-gamma1 in experimental cholesteatoma using Mongolian gerbils.
Phospholipase C (PLC)-gamma1 is known to play a central role in ligand-mediated signal transduction for cell proliferation. The purpose of this study was to elucidate the distribution of PLC-gamma1 in deep meatal skin, retroauricular skin and cholesteatoma matrix of experimental animals. We induced canal ligation cholesteatoma using Mongolian gerbils and investigated the expression of PLC-gamma1 in this model by using Western blot analysis and immunohistochemistry. By Western blot analysis, considerably higher levels of PLC-gamma1 protein were detectable in experimental cholesteatoma. On immunohistochemistry, experimental cholesteatoma showed more intense immunolabeling of PLC-gamma1 protein than deep meatal skin and retroauricular skin. In conclusion, overexpression of PLC-gamma1 may in part contribute to abnormal proliferation and differentiation of experimental cholesteatoma in Mongolian gerbils.
Park
K
K
From the Department of Otolaryngology, Ajou University School of Medicine, Suwon, South Korea. parkkh@madang.ajou.ac.kr
Chun
Y M
YM
Lee
D H
DH
D000818:Animals; D015153:Blotting, Western; D002465:Cell Movement / Q000502:physiology; D018424:Cholesteatoma, Middle Ear / Q000201:enzymology* / Q000503:physiopathology; D004432:Ear, Middle / Q000201:enzymology / Q000473:pathology / Q000503:physiopathology; D005849:Gerbillinae; D007527:Isoenzymes / Q000378:metabolism*; D023421:Models, Animal; D051966:Phospholipase C gamma; D010738:Type C Phospholipases / Q000378:metabolism*
D007527:Isoenzymes; D010738:Type C Phospholipases; D051966:Phospholipase C gamma