title: Structural and serological characterisation of the O-antigenic polysaccharide of the lipopolysaccharide from Acinetobacter strain 96 (DNA group 11).
abstract: A polysaccharide containing D-Manp, L-Fucp (6-deoxygalactopyranose, fucose) and D-GlcpNAc was isolated by mild acid hydrolysis, followed by gel-permeation chromatography, from the lipopolysaccharide derived from Acinetobacter strain 96 (DNA group 11). The structure of the O-antigen was determined by compositional analysis and NMR spectroscopy of the polysaccharide as: [carbohydrate structure see text] A monoclonal antibody obtained after immunization of mice with heat-killed bacteria of Acinetobacter strain 96 was shown to bind to the O-antigen and did not cross-react with any Acinetobacter O-antigen of known structure.
authors.affiliation: Institute for Biological Sciences, NRCC, Ottawa, Ontario, Canada.
authors.forename: E V
authors.initials: EV
authors.lastname: Vinogradov
authors.forename: R
authors.initials: R
authors.lastname: Pantophlet
authors.forename: H
authors.initials: H
authors.lastname: Brade
authors.forename: O
authors.initials: O
authors.lastname: Holst
mesh_terms: D000150:Acinetobacter / Q000737:chemistry* / Q000145:classification; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology; D015153:Blotting, Western; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D003429:Cross Reactions; D000939:Epitopes; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry*; D012703:Serotyping
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000939:Epitopes; D019081:O Antigens