Caged Q-rhodamine dextran: a new photoactivated fluorescent tracer.
An amine-reactive caged rhodamine was synthesized and conjugated to aminodextran. The resulting tracer was injected into a single cell zebrafish embryo, and a portion of the tracer was photolyzed in a single cell after development. The resulting fluorescent cell was imaged by fluorescence microscopy through a single round of cell division.
Gee
K R
KR
Molecular Probes, Inc., 4849 Pitchford Avenue, Eugene, OR 97402, USA. kyle@probes.com
Weinberg
E S
ES
Kozlowski
D J
DJ
D000818:Animals; D003911:Dextrans / Q000138:chemical synthesis* / Q000737:chemistry; D004625:Embryo, Nonmammalian; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D008856:Microscopy, Fluorescence; D010777:Photochemistry; D012235:Rhodamines / Q000138:chemical synthesis* / Q000737:chemistry; D015027:Zebrafish
D003911:Dextrans; D005456:Fluorescent Dyes; D012235:Rhodamines; C051311:rhodamine dextran