Evaluation of polyplexes as gene transfer agents.
Non-viral transfection systems based on the complexes of DNA and polycations ('polyplexes') were evaluated with respect to their effectiveness, toxicity and cell type dependence in a variety of in vitro models. The panel of polycations examined included branched and linear polyethyleneimines, poly[N-ethyl-4-vinyl pyridinium bromide], polyamidoamine dendrimer (Superfect), poly(propyleneimine) dendrimer (Astramol) and a conjugate of Pluronic P123 and polyethyleneimine (P123-g-PEI(2K)), having a graft-block copolymer architecture. Using a panel of cell lines the linear polyethyleneimine ExGen 500, Superfect, branched polyethyleneimine 25 kDa, and P123-g-PEI(2K) were determined as systems displaying highest transfection activity while exhibiting relatively low cytotoxicity. These systems had activity higher than or comparable to lipid transfection reagents (Lipofectin, LipofectAMINE, CeLLFECTIN and DMRIE-C) but did not reveal serum dependence and were less toxic than the lipids. Overall, this study demonstrates good potential of structurally diverse polyplex systems as transfection reagents with relatively low cytotoxicity.
Gebhart
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CL
Department of Pharmaceutical Sciences, College of Pharmacy, 986025 Nebraska Medical Center, Omaha, NE 68198-6025, USA.
Kabanov
A V
AV
D000818:Animals; D002452:Cell Count; D002460:Cell Line; D004247:DNA / Q000008:administration & dosage*; D004586:Electrophoresis; D006801:Humans; D010316:Particle Size; D011073:Polyamines / Q000008:administration & dosage*; D000071228:Polyelectrolytes; D013997:Time Factors; D014162:Transfection
D004247:DNA; D011073:Polyamines; D000071228:Polyelectrolytes