CD4/CXCR4-independent infection of human astrocytes by a T-tropic strain of HIV-1.
HIV-1 establishes a low-level persistent infection in astrocytes. In this study, we studied the susceptibility of a human astrocyte cell line (SVG-A) to infection with luciferase expressing reporter viruses pseudotyped with envelopes derived from five isolates of HIV-1. SVG-A cells were susceptible to infection by a T-cell tropic isolate and the infection was both CD4 and CXCR4 independent. These data confirm the susceptibility of astrocytes to infection with T-tropic strains of HIV-1 and suggest a novel mechanism by which T-tropic strains of HIV can infect cells.
Schweighardt
B
B
Department of Molecular Microbiology and Immunology, Brown University, Providence, Rhode Island 02192, USA.
Shieh
J T
JT
Atwood
W J
WJ
D015526:AIDS Dementia Complex / Q000276:immunology / Q000821:virology*; D001253:Astrocytes / Q000166:cytology / Q000821:virology*; D015704:CD4 Antigens / Q000235:genetics / Q000276:immunology*; D002460:Cell Line; D005333:Fetus / Q000166:cytology; D015870:Gene Expression / Q000276:immunology; D017930:Genes, Reporter; D015497:HIV-1 / Q000145:classification / Q000254:growth & development*; D006801:Humans; D008156:Luciferases / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D019718:Receptors, CXCR4 / Q000235:genetics / Q000276:immunology*; D012703:Serotyping
D015704:CD4 Antigens; D012333:RNA, Messenger; D019718:Receptors, CXCR4; D008156:Luciferases