A study on the human mitochondrial RNA polymerase activity points to existence of a transcription factor B-like protein.
In the present work, the RNA polymerase activity of the human mitochondrial RNA polymerase mature protein (h-mtRPOLm) is shown, and its molecular activity calculated (2.1+/-0.9 min(-1)). An activity analysis of h-mtRPOLm and deleted versions of it has demonstrated that the entire recombinant protein is required for this activity. In addition, h-mtRPOLm alone or in presence of the known mitochondrial transcription factors (human mitochondrial transcription factor A and/or human mitochondrial transcription termination factor) is not able to initiate transcription from the specific human mitochondrial promoters pointing to the existence of a human mitochondrial transcription factor B-like protein.
Prieto-Martín
A
A
Departamento de Bioquímica y Biología Molecular y Celular, Universidad de Zaragoza, Miguel Servet 177, 50013, Zaragoza, Spain.
Montoya
J
J
Martínez-Azorín
F
F
D001483:Base Sequence; D050976:Basic-Leucine Zipper Transcription Factors; D017931:DNA Primers; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D012321:DNA-Directed RNA Polymerases / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008928:Mitochondria / Q000201:enzymology*; D024101:Mitochondrial Proteins; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
D050976:Basic-Leucine Zipper Transcription Factors; D017931:DNA Primers; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; C105178:MTERF1 protein, human; D024101:Mitochondrial Proteins; D009687:Nuclear Proteins; C499631:TFAM protein, human; C499632:TFB1M protein, human; D014157:Transcription Factors; C422562:mitochondrial transcription factor A; D012321:DNA-Directed RNA Polymerases