title: [The expression of HCV RNA polymerase in E.coli and the study of its solubility and antigenicity].
abstract: OBJECTIVE
  To express HCV RNA polymerase and study its soluble condition and antigenicity.
  
  
  METHODS
  We constructed expression vectors pQE-5B-Fl and pQE-5B-C21 and expressed them in E.coli (M15). We analyzed their solubility in different conditions and purified soluble pQE-5B-C21 protein by Ni-NTA column, then detected its antigenicity by ELISA and western blot.
  
  
  RESULTS
  We obtained the purified soluble pQE-5B-C21 protein in the induction conditions of 18 degrees C and the protein was proved to be of good antigenicity by ELISA and western blot.
  
  
  CONCLUSIONS
  The RNA polymerase of HCV expressed in E.coli has good solubility and antigenicity.
authors.affiliation: Department of Applied Molecular Biology, Institute of Microbiology and Epidemiology, Beijing 100071, China.
authors.forename: L
authors.initials: L
authors.lastname: Hou
authors.forename: G
authors.initials: G
authors.lastname: Du
authors.forename: D
authors.initials: D
authors.lastname: Lai
authors.forename: H
authors.initials: H
authors.lastname: Wang
mesh_terms: D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000235:genetics*; D006801:Humans; D012995:Solubility; D017361:Viral Nonstructural Proteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D012324:RNA-Dependent RNA Polymerase
chemical_list: D017361:Viral Nonstructural Proteins; C087036:NS-5 protein, hepatitis C virus; D012324:RNA-Dependent RNA Polymerase