title: Analysis of human urine protein biomarkers via biomolecular interaction analysis mass spectrometry.
abstract: Biomolecular interaction analysis mass spectrometry (BIA/MS) is a two-dimensional chip-based analytical technique geared toward quantitative and qualitative analysis of small volumes of biological samples. Interactions between surface-immobilized ligands and solute-borne analytes are quantitatively viewed in real time through surface plasmon resonance sensing, followed by qualitative matrix-assisted laser desorption/ionization time-of-flight MS analysis of the analyte(s) affinity-retained on the sensor surface. In this work, BIA/MS was used in the detection of a number of protein biomarkers from human urine. Small volumes of human urine were analyzed for cystatin C, beta(2)-microglobulin, urinary protein 1, and retinol-binding protein (RBP). Multiaffinity sensor surfaces were created to simultaneously and rapidly detect all four proteins in a single BIA/MS analysis on a two-flow cell sensor chip configuration. Furthermore, RBP was analyzed separately from both urine and plasma samples. Results indicate that BIA/MS can be used successfully in rapid screening of a number of urinary proteins indicated as putative biological markers for renal dysfunction.
authors.affiliation: Intrinsic Bioprobes Inc, Tempe, AZ 85281, USA. dnedelkov@intrinsicbio.com
authors.forename: D
authors.initials: D
authors.lastname: Nedelkov
authors.forename: R W
authors.initials: RW
authors.lastname: Nelson
mesh_terms: D000906:Antibodies / Q000276:immunology; D015415:Biomarkers / Q000652:urine; D015374:Biosensing Techniques / Q000379:methods*; D055316:Cystatin C; D015891:Cystatins / Q000276:immunology / Q000652:urine*; D006801:Humans; D007674:Kidney Diseases / Q000175:diagnosis / Q000652:urine; D013058:Mass Spectrometry / Q000379:methods*; D012177:Retinol-Binding Proteins / Q000276:immunology / Q000652:urine*; D054839:Retinol-Binding Proteins, Plasma; D001613:beta 2-Microglobulin / Q000276:immunology / Q000652:urine*
chemical_list: D000906:Antibodies; D015415:Biomarkers; C525030:CST3 protein, human; D055316:Cystatin C; D015891:Cystatins; D012177:Retinol-Binding Proteins; D054839:Retinol-Binding Proteins, Plasma; D001613:beta 2-Microglobulin