title: High-resolution magic angle spinning 1H-NMR spectroscopy studies on the renal biochemistry in the bank vole (Clethrionomys glareolus) and the effects of arsenic (As3+) toxicity.
abstract: 1. High-resolution magic angle spinning (MAS) 1H-NMR spectroscopy was used to study renal metabolism and the toxicity of As3+, a common environmental contaminant, in the bank vole (Clethrionomys glareolus), a wild species of rodent. 2. Following a 14-day exposure to an environmentally relevant dose of As2O3 (28 mg kg(-1) feed), voles displayed tissue damage at autopsy. MAS 1H spectra indicated abnormal lipid profiles in these samples. 3. Tissue necrosis was also evident from measurements of the apparent diffusion coefficient of water in the intact tissue using MAS 1H diffusion-weighted spectroscopy, its first application to toxicology. 4. Comparison of renal tissue from the wood mouse (Apodemus sylvaticus) exposed to identical exposure levels of As3+ suggested that the bank vole is particularly vulnerable to As3+ toxicity.
authors.affiliation: Biological Chemistry, Division of Biomedical Sciences, Imperial College, London, UK. j.griffin@ic.ac.uk
authors.forename: J L
authors.initials: JL
authors.lastname: Griffin
authors.forename: L
authors.initials: L
authors.lastname: Walker
authors.forename: R F
authors.initials: RF
authors.lastname: Shore
authors.forename: J K
authors.initials: JK
authors.lastname: Nicholson
mesh_terms: D000409:Alanine / Q000032:analysis; D000818:Animals; D001151:Arsenic / Q000633:toxicity*; D003411:Arvicolinae; D003401:Creatine / Q000032:analysis; D018698:Glutamic Acid / Q000032:analysis; D005973:Glutamine / Q000032:analysis; D007668:Kidney / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology; D007674:Kidney Diseases / Q000139:chemically induced*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008297:Male; D009115:Muridae; D009336:Necrosis; D010767:Phosphorylcholine / Q000032:analysis; D013045:Species Specificity
chemical_list: D005973:Glutamine; D010767:Phosphorylcholine; D018698:Glutamic Acid; D003401:Creatine; D001151:Arsenic; D000409:Alanine