An ultrastructural study of osteoclasts and chondroclasts in poorly calcified mandible induced by high doses of strontium diet to fetal mice.
A high dose strontium diet was fed to fetal mice from day 1 of gestation to birth in order to investigate the ultrastructural changes of osteoclasts/chondroclasts when associated with poorly calcified bone/cartilage. Calcification in the mandibular bone and condylar cartilage was extensively inhibited by this diet. Multinucleated osteoclasts and chondroclasts were observed on the mandibular alveolar bone and in the resorption area of the condylar cartilage, respectively. However, both cell types never formed ruffled borders and clear zones at the cell surfaces facing the matrices indicative of bone resorption, although they had well-developed organelles and vacuoles. Furthermore, they revealed signs of phagocytosis of the matrix vesicles. These results indicate that osteoclasts/chondroclasts can exhibit phagocytotic activity in response to requirements.
Shibata
S
S
Department of Maxillofacial Biology, Graduate School, Tokyo Medical and Dental University, Japan. S.Shibata.mfa@tmd.ac.jp
Yamashita
Y
Y
D000818:Animals; D000831:Animals, Newborn; D002113:Calcification, Physiologic / Q000528:radiation effects*; D002356:Cartilage / Q000473:pathology / Q000528:radiation effects* / Q000648:ultrastructure; D019902:Chondrocytes / Q000528:radiation effects / Q000648:ultrastructure*; D005260:Female; D008334:Mandible / Q000473:pathology / Q000528:radiation effects* / Q000648:ultrastructure; D051379:Mice; D008813:Mice, Inbred ICR; D010010:Osteoclasts / Q000528:radiation effects / Q000648:ultrastructure*; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D013324:Strontium / Q000633:toxicity*
D013324:Strontium