Binding of regulatory 14-3-3 proteins to the C terminus of the plant plasma membrane H+ -ATPpase involves part of its autoinhibitory region.
The plant plasma membrane H+ -ATPase is activated by the binding of 14-3-3 proteins to its extreme C-terminal amino acids (YTV) and phosphorylation of the penultimate threonine (YpTV) is necessary for this interaction in vivo. However, in the presence of the fungal toxin fusicoccin (FC), binding of 14-3-3 proteins occurs independently of phosphorylation but still involves the YTV motif. Since FC exclusively binds to the complex consisting of both 14-3-3 homologs and the C-terminal domain of the H+ -ATPase, the toxin was used as a tool to reveal potential protein-protein interaction sites in the enzyme's C terminus. We performed in vitro interaction studies by applying various C-terminal parts of the H+ -ATPase PMA2 from Nicotiana plumbaginifolia expressed as glutathione S-transferase fusion peptides in E. coli. Interestingly, the PMA2 region encompassing residues 905-922 is implicated in FC-dependent binding of 14-3-3 homologs. Recently, part of this region has been shown to contribute to the autoinhibitory action of the PMA2 C terminus. Site-directed mutagenesis of individual amino acids localized within this region resulted in a drastic decrease in FC-dependent binding of 14-3-3 proteins. Furthermore, by expressing the corresponding mutants of PMA2 in yeast, we observed a reduced capability of the mutant enzymes to functionally replace the endogenous H+ -ATPase. Notably, the decreased activity of the mutant enzymes was accompanied by a weakened binding of yeast 14-3-3 homologs to the plasma membrane of transformed cells. Taken together, our results suggest that a section of the autoinhibitory C-terminal PMA2 region contributes to binding of activatory 14-3-3 proteins in the absence of FC.
Jelich-Ottmann
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Lehrstuhl für Pflanzenphysiologie, Ruhr-Universität, D-44801 Bochum, Germany.
Weiler
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Oecking
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D000595:Amino Acid Sequence; D017360:Arabidopsis; D001665:Binding Sites; D002462:Cell Membrane / Q000201:enzymology*; D006027:Glycosides / Q000494:pharmacology; D008565:Membrane Proteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D009183:Mycotoxins / Q000494:pharmacology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D006180:Proton-Translocating ATPases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D014026:Nicotiana; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism*
D006027:Glycosides; D008565:Membrane Proteins; D009183:Mycotoxins; D010940:Plant Proteins; D011994:Recombinant Proteins; C007808:fusicoccin; C439670:T14-3c protein, Nicotiana tabacum; D014446:Tyrosine 3-Monooxygenase; D006180:Proton-Translocating ATPases