Modulation of immunostimulatory activity of CpG oligonucleotides by site-specific deletion of nucleobases.
The effect of nucleobase deletion in the 3'- or the 5'-flanking sequence to a CpG-motif on immunostimulatory activity of CpG-containing oligonucleotides was examined by cell proliferation, secretion of IL-12 and IL-6 in mouse spleen cell cultures, and by spleen enlargement in mice. Deletion of one or two nucleobases in the 3'-flanking sequence to a CpG-motif at certain positions did not affect immunostimulatory activity, while similar deletions in the 5'-flanking sequence increased immunostimulatory activity compared with the parent oligo.
Yu
D
D
Hybridon, Inc., 345 Vassar Street, Cambridge, MA 02139, USA.
Kandimalla
E R
ER
Zhao
Q
Q
Cong
Y
Y
Agrawal
S
S
D024509:3' Flanking Region; D024506:5' Flanking Region; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D018899:CpG Islands / Q000276:immunology*; D007274:Injections, Intraperitoneal; D051379:Mice; D008807:Mice, Inbred BALB C; D009841:Oligonucleotides / Q000737:chemistry / Q000276:immunology* / Q000494:pharmacology*; D009929:Organ Size / Q000187:drug effects; D017384:Sequence Deletion; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D013329:Structure-Activity Relationship
D009841:Oligonucleotides