Separation of low molecular weight proteins with SDS-PAGE using taurine as a new trailing ion.
Taurine as an alternative trailing ion for tricine yields an identical resolution but reduces the running time by 15% and the power consumption by 15% compared to tricine. Therefore cooling of gels is more effective in SDS PAGE with taurine and artefacts due to oxidation of proteins can thus be reduced.
Ehlermann
P
P
Department of Medicine II, Medical University of Lübeck, Germany.
Redweik
U
U
Blau
N
N
Heizmann
C W
CW
Katus
H A
HA
Remppis
A
A
D016477:Artifacts; D002021:Buffers; D002135:Calcium-Binding Proteins / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005782:Gels; D005998:Glycine / Q000031:analogs & derivatives*; D007202:Indicators and Reagents; D008970:Molecular Weight; D010084:Oxidation-Reduction; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification*; D013654:Taurine
D002021:Buffers; D002135:Calcium-Binding Proteins; D005782:Gels; D007202:Indicators and Reagents; D011506:Proteins; D013654:Taurine; D005998:Glycine; C100184:tricine