title: Cutting edge: dichotomy of homing receptor dependence by mucosal effector B cells: alpha(E) versus L-selectin.
abstract: The common mucosal immune system may be compartmentalized because lymphocyte homing to the upper respiratory tract appears to be mediated by L-selectin interactions rather than alpha(4)beta(7) interactions, as is the case for gut-associated lymphoreticular tissue. To assess the role of L-selectin in effector B cell immunity, L-selectin-deficient mice were intranasally immunized with cholera toxin (CT), and mucosal immune responses were compared with C57BL/6 mice. The absence of L-selectin correlated with a reduction in CT-specific secretory-IgA responses in nasal passages and reproductive tract, but not intestinal lamina propria. Cell sorting experiments showed that an L-selectin-dependent subset was responsible for CT-specific responses in nasal passages and reproductive tract, whereas an alpha(E)beta(7)(+) B cell subset was responsible for L-selectin-independent intestinal immunity. This study provides evidence for compartmentalization of the common mucosal immune system into "intestinal" vs "nonintestinal" effector sites.
authors.affiliation: Veterinary Molecular Biology, Montana State University, Bozeman, MT 59717, USA.
authors.forename: K L
authors.initials: KL
authors.lastname: Csencsits
authors.forename: N
authors.initials: N
authors.lastname: Walters
authors.forename: D W
authors.initials: DW
authors.lastname: Pascual
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism*; D001402:B-Lymphocytes / Q000276:immunology*; D002772:Cholera Toxin / Q000633:toxicity; D005260:Female; D018928:Immunity, Mucosal; D039001:Integrin alpha Chains; D007413:Intestinal Mucosa / Q000276:immunology; D019041:L-Selectin / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009297:Nasal Mucosa / Q000276:immunology; D016201:Receptors, Lymphocyte Homing / Q000378:metabolism*; D014621:Vagina / Q000276:immunology
chemical_list: D015703:Antigens, CD; D039001:Integrin alpha Chains; D016201:Receptors, Lymphocyte Homing; C091367:alpha E integrins; D019041:L-Selectin; D002772:Cholera Toxin