Dual role of the fringe connection gene in both heparan sulphate and fringe-dependent signalling events.
The precise regulation of growth factor signalling is crucial to the molecular control of development in Drosophila. Post-translational modification of signalling molecules is one of the mechanisms that modulate developmental signalling specificity. We describe a new gene, fringe connection (frc), that encodes a nucleotide-sugar transporter that transfers UDP-glucuronic acid, UDP-N-acetylglucosamine and possibly UDP-xylose from the cytoplasm into the lumen of the endoplasmic reticulum/Golgi. Embryos with the frc mutation display defects in Wingless, Hedgehog and fibroblast growth factor signalling. Clonal analysis shows that fringe-dependent Notch signalling is disrupted in frc mutant tissue.
Selva
E M
EM
Department of Genetics, Howard Hughes Medical Institute, Harvard Medical School, Boston, MA 02115, USA.
Hong
K
K
Baeg
G H
GH
Beverley
S M
SM
Turco
S J
SJ
Perrimon
N
N
Häcker
U
U
D000595:Amino Acid Sequence; D000818:Animals; D003593:Cytoplasm / Q000378:metabolism; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000196:embryology / Q000235:genetics* / Q000254:growth & development; D004721:Endoplasmic Reticulum / Q000378:metabolism; D016695:Glycosyltransferases / Q000235:genetics / Q000378:metabolism*; D006056:Golgi Apparatus / Q000378:metabolism; D006497:Heparan Sulfate / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D009024:Morphogenesis; D017351:N-Acetylglucosaminyltransferases; D010641:Phenotype; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction / Q000502:physiology*; D014535:Uridine Diphosphate Glucuronic Acid / Q000378:metabolism; D014537:Uridine Diphosphate N-Acetylglucosamine / Q000378:metabolism; D014540:Uridine Diphosphate Xylose / Q000378:metabolism; D014921:Wings, Animal / Q000196:embryology / Q000254:growth & development
D029721:Drosophila Proteins; D016695:Glycosyltransferases; D006497:Heparan Sulfate; D017351:N-Acetylglucosaminyltransferases; D014535:Uridine Diphosphate Glucuronic Acid; D014537:Uridine Diphosphate N-Acetylglucosamine; D014540:Uridine Diphosphate Xylose; C090576:fng protein, Drosophila