Dehalogenation of chlorinated hydroxybiphenyls by fungal laccase.
We have investigated the transformation of chlorinated hydroxybiphenyls by laccase produced by Pycnoporus cinnabarinus. The compounds used were transformed to sparingly water-soluble colored precipitates which were identified by gas chromatography-mass spectrometry as oligomerization products of the chlorinated hydroxybiphenyls. During oligomerization of 2-hydroxy-5-chlorobiphenyl and 3-chloro-4-hydroxybiphenyl, dechlorinated C---C-linked dimers were formed, demonstrating the dehalogenation ability of laccase. In addition to these nonhalogenated dimers, both monohalogenated and dihalogenated dimers were identified.
Schultz
A
A
Institute of Microbiology and Molecular Biology, Ernst Moritz Arndt University of Greifswald, D-17487 Greifswald, Germany. asgard.schultz@web.de
Jonas
U
U
Hammer
E
E
Schauer
F
F
D001487:Basidiomycota / Q000201:enzymology* / Q000254:growth & development; D001673:Biodegradation, Environmental; D001713:Biphenyl Compounds / Q000737:chemistry / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D042845:Laccase; D009682:Magnetic Resonance Spectroscopy; D010088:Oxidoreductases / Q000378:metabolism*; D011078:Polychlorinated Biphenyls / Q000737:chemistry / Q000378:metabolism*
D001713:Biphenyl Compounds; D011078:Polychlorinated Biphenyls; D010088:Oxidoreductases; D042845:Laccase