Evidence of a tissue-restricting DNA regulatory element in the mouse IRBP promoter.
The expression of interphotoreceptor retinoid binding protein (IRBP) is limited to photoreceptor cells of the retina and pinealocytes of the pineal gland. We sought to define cis-elements of the mouse IRBP 5' flanking region that are required for this restricted activity. In vitro transient transfections of retinoblastoma and neuroblastoma cells and in vivo experiments with transgenic Xenopus laevis indicate that -1783/+101 and -156/+101 IRBP gene fragments directed expression predominantly to the retina and pineal, with minor neuronal expression elsewhere. In contrast, a -70/+101 fragment was less restrictive in controlling expression, exhibiting activity not only in retina, but also in forebrain, hindbrain, spinal cord, and motor neurons innervating gills.
Boatright
J H
JH
Department of Ophthalmology, Emory Eye Center, Atlanta, University of Emory, GA 30322, USA. jboatri@emory.edu
Knox
B E
BE
Jones
K M
KM
Stodulkova
E
E
Nguyen
H T
HT
Padove
S A
SA
Borst
D E
DE
Nickerson
J M
JM
D000818:Animals; D030801:Animals, Genetically Modified; D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D005136:Eye Proteins / Q000235:genetics*; D015870:Gene Expression; D006801:Humans; D051379:Mice; D011401:Promoter Regions, Genetic; D012177:Retinol-Binding Proteins / Q000235:genetics*; D014982:Xenopus laevis; D000099214:Retinol-Binding Proteins, Interstitial
D015500:Chloramphenicol O-Acetyltransferase; D005136:Eye Proteins; D012177:Retinol-Binding Proteins; D000099214:Retinol-Binding Proteins, Interstitial