title: The salivary adenosine/AMP content of Phlebotomus argentipes Annandale and Brunetti, the main vector of human kala-azar.
abstract: Adenosine and AMP in the salivary glands of the sand fly Phlebotomus argentipes were characterized by reversed-phase high-pressure liquid chromatography with diode-array detection and mass spectrometry. AMP and adenosine were measured in individual salivary gland pairs, yielding 76.8 +/- 8.6 and 380 +/- 25 pmoles per pair of salivary glands, respectively (mean +/- SE, n = 12). These values decrease to 45 +/- 7 and 181 +/- 21 pmoles following a blood meal, indicating that AMP and adenosine were secreted. Because adenosine and AMP have anti-platelet, vasodilatory, and immunomodulatory properties, it is proposed that these salivary nucleotides help the fly to blood feed and may affect Leishmania transmission.
authors.affiliation: Section of Medical Entomology, Laboratory of Parasitic Diseases, Bethesda, Maryland 20892-0425, USA.
authors.forename: J M
authors.initials: JM
authors.lastname: Ribeiro
authors.forename: G
authors.initials: G
authors.lastname: Modi
mesh_terms: D000241:Adenosine / Q000032:analysis*; D000249:Adenosine Monophosphate / Q000032:analysis*; D000276:Adjuvants, Immunologic / Q000032:analysis; D000818:Animals; D000925:Anticoagulants / Q000032:analysis; D004199:Disease Vectors; D005260:Female; D007898:Leishmaniasis, Visceral / Q000635:transmission; D010691:Phlebotomus; D012469:Salivary Glands / Q000737:chemistry*; D014665:Vasodilator Agents / Q000032:analysis
chemical_list: D000276:Adjuvants, Immunologic; D000925:Anticoagulants; D014665:Vasodilator Agents; D000249:Adenosine Monophosphate; D000241:Adenosine