title: Structural variability and originality of the Bordetella endotoxins.
abstract: Structural studies of Bordetella endotoxins (LPSs) have revealed remarkable differences: (i) between their LPSs and those of other bacterial pathogens; (ii) among the LPSs of the seven identified Bordetella species; and (iii) among the LPSs of some Bordetella strains. The lipid As have the "classical" bisphosphorylated diglucosamine backbone but tend to have fewer and species-specific fatty acid components compared to those of other genera. Nevertheless, three strains of B. bronchiseptica have at least three different fatty acid distributions; however, the recently identified B. hinzii and B. trematum LPSs had identical lipid A structures. The B. pertussis core is a dodecasaccharide multi-branched structure bearing amino and carboxylic groups. Another unusual feature is the presence of free amino sugars in the central core region and a complex distal trisaccharide unit containing five amino groups of which four are acetylated and one is methylated. The B. pertussis LPS does not have O-chains and that of B. trematum had only a single O-unit, unlike the LPSs of all the other species of the smooth-type. The O-chain-free cores of non-B. pertussis LPSs were always built on the B. pertussis core model but most were species-specifically incomplete. The LPS structures of three B. bronchiseptica strains were found to be different from each other. The O-chains of B. bronchiseptica and B. parapertussis were almost identical and had some features in common with B. hinzii O-chain. Serological analyses are consistent with the determined LPS structures.
authors.affiliation: Equipe 'Endotoxines', UMR 8619, Centre National de la Recherche Scientifique, Biochimie, Université de Paris-Sud, Orsay, France. martine.caroff@bbmpc.u-psud.fr
authors.forename: M
authors.initials: M
authors.lastname: Caroff
authors.forename: L
authors.initials: L
authors.lastname: Aussel
authors.forename: H
authors.initials: H
authors.lastname: Zarrouk
authors.forename: A
authors.initials: A
authors.lastname: Martin
authors.forename: J C
authors.initials: JC
authors.lastname: Richards
authors.forename: H
authors.initials: H
authors.lastname: Thérisod
authors.forename: M B
authors.initials: MB
authors.lastname: Perry
authors.forename: D
authors.initials: D
authors.lastname: Karibian
mesh_terms: D001884:Bordetella / Q000235:genetics / Q000276:immunology*; D002240:Carbohydrate Sequence; D002855:Chromatography, Thin Layer; D004591:Electrophoresis, Polyacrylamide Gel; D004731:Endotoxins / Q000737:chemistry* / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008050:Lipid A / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods; D008969:Molecular Sequence Data; D015394:Molecular Structure
chemical_list: D004731:Endotoxins; D005227:Fatty Acids; D008050:Lipid A