title: Intraoperative sentinel lymph node mapping in patients with colon cancer.
abstract: BACKGROUND
  The sentinel lymph node (SLN) mapping technique has been used in breast cancer and melanoma, and was recently described for colon cancer.
  
  
  METHODS
  Thirty-five patients with colon cancer underwent intraoperative SLN mapping. One milliliter of 1% isosulfan blue was injected subserosally around the tumor. The first nodal area that was highlighted with blue was identified as the SLN. All lymph nodes underwent examination with hematoxylin and eosin (H&E) stain. SLNs underwent additional sectioning and were stained with CAM 5.2.
  
  
  RESULTS
  Lymphatic mapping adequately identified the SLN in 25 patients (71%). In the 15 cases where the SLNs were negative for metastases, all other non-SLNs were also negative (0% false negative rate). The SLN was the only site of metastases in 6 (17%) of 35 patients. CAM 5.2 staining provided the only evidence of micrometastases in 4 (11%) of 35 patients.
  
  
  CONCLUSIONS
  Intraoperative SLN mapping is a feasible technique with a reasonable SLN identification rate (71%). The absence of metastases in the SLNs accurately predicts the status of the non-SLNs. Tumors in 11% of patients were upstaged by the demonstration of micrometastatic involvement, and these patients may benefit from further adjuvant chemotherapy.
authors.affiliation: Department of Surgery, Mount Sinai Medical Center, 4300 Alton Road, Miami Beach, FL 33140, USA. jcparamo@med411.com
authors.forename: J C
authors.initials: JC
authors.lastname: Paramo
authors.forename: J
authors.initials: J
authors.lastname: Summerall
authors.forename: C
authors.initials: C
authors.lastname: Wilson
authors.forename: A
authors.initials: A
authors.lastname: Cabral
authors.forename: I
authors.initials: I
authors.lastname: Willis
authors.forename: H
authors.initials: H
authors.lastname: Wodnicki
authors.forename: R
authors.initials: R
authors.lastname: Poppiti
authors.forename: T W
authors.initials: TW
authors.lastname: Mesko
mesh_terms: D000230:Adenocarcinoma / Q000473:pathology* / Q000601:surgery; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D003110:Colonic Neoplasms / Q000473:pathology* / Q000601:surgery; D005260:Female; D006801:Humans; D007430:Intraoperative Care; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies; D012394:Rosaniline Dyes; D012680:Sensitivity and Specificity; D021701:Sentinel Lymph Node Biopsy / Q000379:methods*
chemical_list: D012394:Rosaniline Dyes; C025484:iso-sulfan blue