Capillary-based fully integrated and automated system for nanoliter polymerase chain reaction analysis directly from cheek cells.
A miniaturized, integrated and automated system based on capillary fluidics has been developed for nanoliter DNA analysis directly from cheek cells. All steps for DNA analysis, including injecting aqueous reagents and DNA samples, mixing the solutions together, thermal cell lysis, polymerase chain reaction (PCR), transfer and injection of PCR product, separation, sizing and detection of those products are performed in a capillary-based integrated system. A small amount of cheek cells collected by a plastic toothpick is directly dissolved in the PCR cocktail in a plastic vial or mixed on-line with a small volume of PCR cocktail (125 nl) in the capillary. After thermal cell lysis and PCR in a microthermal cycler, the DNA fragments are mixed with DNA size standards and transferred to a micro-cross for injection and separation by capillary gel electrophoresis. Programmable syringe pumps, switching valves, multiposition and freeze-thaw valves are used for microfluidic control in the entire system. This work establishes the feasibility of performing all the steps of DNA analysis from real samples in a capillary-based nanoliter integrated system.
He
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Ames Laboratory-US Department of Energy and Department of Chemistry, Iowa State University, 50011, USA.
Zhang
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YH
Yeung
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ES
D001331:Automation; D001483:Base Sequence; D002610:Cheek; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D017931:DNA Primers; D006801:Humans; D009061:Mouth Mucosa / Q000166:cytology* / Q000378:metabolism; D036103:Nanotechnology; D016133:Polymerase Chain Reaction / Q000295:instrumentation* / Q000379:methods; D017422:Sequence Analysis, DNA / Q000295:instrumentation* / Q000379:methods
D017931:DNA Primers; D004247:DNA