title: In vivo 23Na nuclear magnetic resonance study of maintenance of a sodium gradient in the ruminal bacterium Fibrobacter succinogenes S85.
abstract: Sodium gradients (DeltapNa) were measured in resting cells of Fibrobacter succinogenes by in vivo 23Na nuclear magnetic resonance using Tm(DOTP)5- [thulium(III) 1,4,7,10-tetraazacyclododecane-N',N",N"'-tetramethylenephosphonate] as the shift reagent. This bacterium was able to maintain a DeltapNa of -55 to -40 mV for extracellular sodium concentrations ranging from 30 to 200 mM. Depletion of Na+ ions during the washing steps led to irreversible damage (modification of glucose metabolism and inability to maintain a sodium gradient).
authors.affiliation: Laboratoire de Synthèse, Electrosynthèse et Etude de Systèmes à Intérêt Biologique, UMR 6504, Université Blaise Pascal-CNRS, 63177 Aubière cedex, France.
authors.forename: V
authors.initials: V
authors.lastname: Schwaab
authors.forename: C
authors.initials: C
authors.lastname: Matheron
authors.forename: A M
authors.initials: AM
authors.lastname: Delort
authors.forename: G
authors.initials: G
authors.lastname: Gaudet
authors.forename: E
authors.initials: E
authors.lastname: Forano
mesh_terms: D000818:Animals; D001421:Bacteria, Anaerobic / Q000502:physiology*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D009942:Organometallic Compounds / Q000378:metabolism; D009943:Organophosphorus Compounds / Q000378:metabolism; D012417:Rumen / Q000382:microbiology*; D012965:Sodium Chloride / Q000378:metabolism*; D012975:Sodium Isotopes / Q000378:metabolism
chemical_list: D009942:Organometallic Compounds; D009943:Organophosphorus Compounds; D012975:Sodium Isotopes; C069929:thulium(III) 1,4,7,10-tetraazacyclododecane-N,N',N'',N'''-tetramethylenephosphonate; D012965:Sodium Chloride