title: Effect of mechanical deformation of neutrophils on their CD18/ICAM-1-dependent adhesion.
abstract: Mechanical deformation of polymorphonuclear leukocytes (PMN) changes their expression of the surface adhesion molecule CD11b/CD18. We tested the hypothesis that mechanical deformation of PMN enhances their adhesiveness. Purified human PMN were deformed through either 5- or 3-microm polycarbonate membrane filters and allowed to adhere to 96-well plates coated with human recombinant intercellular adhesion molecule-1 (ICAM-1). Flow cytometric studies showed that deformation of PMN increased CD11b/CD18 expression (P < 0.01). PMN adhesion to ICAM-1-coated plates was dependent on the magnitude of cell deformation (5 microm, 63.8 +/- 8.1%, P < 0.04; 3 microm, 232.4 +/- 20.9%, P < 0.01). Priming of PMN (0.5 nM N-formyl-methionyl-leucyl-phenylalanine) before deformation (5 microm) increased PMN adhesion (63.8 +/- 8.1 vs. 105.3 +/- 16.4%; P < 0.04). Stimulation (5% zymosan-activated plasma) of PMN after deformation resulted in increased adhesion, and the degree of increase was dependent on the magnitude of PMN deformation (stimulation, 50.6 +/- 4%; 5-microm filtration and stimulation, 62.9 +/- 6.6%; 3-microm filtration and stimulation, 249.9 +/- 24.2%; P < 0.01). This study shows that mechanical deformation of PMN causes an increase in PMN adhesiveness to ICAM-1 that was enhanced by both priming of PMN before deformation and stimulation after cell deformation.
authors.affiliation: Pulmonary Research Laboratory, St. Paul's Hospital, University of British Columbia, Vancouver, British Columbia, Canada V6Z 1Y6.
authors.forename: G J
authors.initials: GJ
authors.lastname: Anderson
authors.forename: W T
authors.initials: WT
authors.lastname: Roswit
authors.forename: M J
authors.initials: MJ
authors.lastname: Holtzman
authors.forename: J C
authors.initials: JC
authors.lastname: Hogg
authors.forename: S F
authors.initials: SF
authors.lastname: Van Eeden
mesh_terms: D018821:CD18 Antigens / Q000378:metabolism*; D002448:Cell Adhesion / Q000276:immunology; D048429:Cell Size / Q000502:physiology; D005374:Filtration; D005434:Flow Cytometry; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000494:pharmacology*; D016177:Macrophage-1 Antigen / Q000378:metabolism; D008833:Microcirculation; D009504:Neutrophils / Q000166:cytology* / Q000378:metabolism; D011312:Pressure; D011652:Pulmonary Circulation
chemical_list: D018821:CD18 Antigens; D016177:Macrophage-1 Antigen; D018799:Intercellular Adhesion Molecule-1