Loss of MHC class II inducibility in hyperplastic tissue in Rb-defective mice.
Retinoblastoma gene (Rb) defects occur frequently in human tumors. Studies of Rb-defective human tumor cell lines and Rb-/- murine embryonic fibroblasts demonstrate that Rb is required for interferon-gamma (IFN-gamma) induced major histocompatibility complex (MHC) class II expression. MHC class II expressing tumors generate anti-tumor immune responses associated with tumor-specific infiltrating lymphocytes. The role of Rb in IFN-gamma induced MHC class II expression on an endogenous tumor was examined by immunohistochemical staining for IAbeta and Rb on tissues from Rb+/- mice. MHC class II IAbeta is not induced by IFN-gamma in Rb-deficient neoplastic cells, but remains inducible in related normal tissue.
Eason
D D
DD
Department of Biochemistry and Molecular Biology, University of South Florida, College of Medicine, MDC 7, 12901 Bruce B. Downs Boulevard, Tampa, FL 33612, USA.
Coppola
D
D
Livingston
S
S
Shepherd
A T
AT
Blanck
G
G
D000818:Animals; D005260:Female; D005786:Gene Expression Regulation; D005802:Genes, MHC Class II; D016161:Genes, Retinoblastoma / Q000235:genetics*; D000949:Histocompatibility Antigens Class II / Q000096:biosynthesis* / Q000235:genetics; D006965:Hyperplasia / Q000276:immunology / Q000378:metabolism; D007150:Immunohistochemistry; D007371:Interferon-gamma / Q000494:pharmacology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D011230:Precancerous Conditions / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D016160:Retinoblastoma Protein / Q000172:deficiency* / Q000502:physiology
D000949:Histocompatibility Antigens Class II; D016160:Retinoblastoma Protein; D007371:Interferon-gamma