title: The SEL-12 presenilin mediates induction of the Caenorhabditis elegans uterine pi cell fate.
abstract: During Caenorhabditis elegans hermaphrodite development, the anchor cell induces the vulva and the uterine pi cells whose daughters connect to the vulva, thereby organizing the uterine-vulval connection. Both the initial selection of a single anchor cell during the anchor cell vs. ventral uterine precursor cell decision and the subsequent induction of the pi cell fate by the anchor cell are mediated by the lin-12 gene. Members of the presenilin gene family can cause early onset Alzheimer's disease when mutated and are also required for LIN-12/Notch signaling during development. We have shown that, in C. elegans, mutation of the sel-12-encoded presenilin results in pi cell induction defects. By contrast, other lin-12-mediated cell fate decisions occur normally in sel-12 mutants due to the redundant function of a second C. elegans presenilin called HOP-1. We found that the sel-12 egg-laying defect was partially rescued by expression of the sel-12 gene in the pi cells. sel-12-mediated pi cell fate specification provides a useful system for the analysis of presenilin function at single cell resolution.
authors.affiliation: Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Program in Developmental Biology, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA.
authors.forename: H N
authors.initials: HN
authors.lastname: Cinar
authors.forename: K L
authors.initials: KL
authors.lastname: Sweet
authors.forename: K E
authors.initials: KE
authors.lastname: Hosemann
authors.forename: K
authors.initials: K
authors.lastname: Earley
authors.forename: A P
authors.initials: AP
authors.lastname: Newman
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000502:physiology*; D029742:Caenorhabditis elegans Proteins; D005260:Female; D015801:Helminth Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D009154:Mutation; D010058:Oviposition; D014599:Uterus / Q000166:cytology / Q000196:embryology*
chemical_list: D029742:Caenorhabditis elegans Proteins; D015801:Helminth Proteins; C109040:Hop-1 protein, C elegans; D008565:Membrane Proteins; C095907:SEL-12 protein, C elegans