The occurrence of geometric polyprenol isomers in the rubber-producing plant, Eucommia ulmoides Oliver.
The chain length and geometric isomerism of polyprenols from Eucommia ulmoides Oliver were analyzed using supercritical fluid chromatography. After intensive effort to establish separation conditions for geometric isomers, a phenyl-bonded silica gel-packed column was found that cleanly separated poly-trans and -cis prenols. The presence of long-chain poly-trans prenols (>9 mers) was confirmed for the first time in plants. Trans isomers were found in the leaf, seed coat, and root, but not in the bark and seed. Poly-trans prenols in this plant may act as intermediates for trans-polyisoprene biosynthesis.
Bamba
T
T
Department of Biotechnology, Graduate School of Engineering, Osaka University, Japan.
Fukusaki
E
E
Kajiyama
S
S
Ute
K
K
Kitayama
T
T
Kobayashi
A
A
D002851:Chromatography, High Pressure Liquid; D025924:Chromatography, Supercritical Fluid; D007536:Isomerism; D009682:Magnetic Resonance Spectroscopy; D018515:Plant Leaves / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D010944:Plants / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry; D013729:Terpenes / Q000032:analysis* / Q000737:chemistry
D013729:Terpenes