Kinetic studies of Rhizopus oryzae lipase using monomolecular film technique.
Rhizopus oryzae lipase (ROL) was found to be a true lipase. This enzyme presents the interfacial activation phenomenon. The N-terminal amino acid sequence of ROL was compared to those of rhizopus lipases. Purified ROL possesses the same N-terminal sequence as the mature Rhizopus niveus lipase (RNL). This sequence was found in the last 28 amino acids of the propeptide sequence derived from the cDNA of Rhizopus delemar lipase (RDL). Using the baro-stat method, we have measured the hydrolysis rate of dicaprin films by ROL as a function of surface pressure. Our results show that Rhizopus oryzae lipase is markedly stereoselective of the sn-3 position of the 2,3 enantiomer of dicaprin. Polyclonal antibodies (PAB) directed against ROL have been produced and purified by immunoaffinity. The effects of these PAB on the interfacial behavior of ROL were determined. The immunoblot analysis with polyclonal antibodies anti-ROL (PAB anti-ROL) and various lipases shows a cross-immunoreactivity between the lipase from the rhizopus family (Rhizopus delemar lipase and Rhizopus arrhizus lipase).
Ben Salah
A
A
Unité de Lipolyse Enzymatique, ENIS BPW, 3038, Sfax, Tunisia. ytgargouri@yahoo.fr
Sayari
A
A
Verger
R
R
Gargouri
Y
Y
D000595:Amino Acid Sequence; D000906:Antibodies / Q000276:immunology / Q000494:pharmacology; D001274:Atmospheric Pressure; D003429:Cross Reactions; D004075:Diglycerides / Q000737:chemistry / Q000378:metabolism*; D006868:Hydrolysis; D015151:Immunoblotting; D007525:Isoelectric Focusing; D007526:Isoelectric Point; D007700:Kinetics; D008049:Lipase / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism*; D008969:Molecular Sequence Data; D012233:Rhizopus / Q000201:enzymology*; D013237:Stereoisomerism; D013379:Substrate Specificity
D000906:Antibodies; D004075:Diglycerides; D008049:Lipase