Bioconcentration of nonylphenol in fathead minnows (Pimephales promelas).
Bioconcentration of p-nonylphenol (NP) by fathead minnows was determined under laboratory conditions. Fish were exposed continuously for 42 days to 0.33, 0.93 and 2.36 microg NP/l in a flow-through system. NP was Soxhlet extracted from whole fish homogenates with dichloromethane (DCM). The resulting extract was concentrated and bulk lipids removed by gel permeation and silica-gel chromatography. Compounds were identified and quantified by reverse-phase high-pressure liquid chromatography (RP-HPLC) with fluorescence detection. Mass spectrometry was used for verification of peak assignments. Bioconcentration factors (BCFs) ranged from 245 to 380.
Snyder
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SA
Department of Zoology, Institute of Environmental Toxicology, National Food Safety and Toxicology Center, Michigan State University, East Lansing 48824-1311, USA. shane.snyder@lvvwd.com
Keith
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TL
Pierens
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SL
Snyder
E M
EM
Giesy
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JP
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003530:Cyprinidae; D015180:Electrophoresis, Gel, Two-Dimensional; D008055:Lipids; D013058:Mass Spectrometry; D010636:Phenols / Q000493:pharmacokinetics*; D014018:Tissue Distribution; D014874:Water Pollutants, Chemical / Q000493:pharmacokinetics*
D008055:Lipids; D010636:Phenols; D014874:Water Pollutants, Chemical; C025256:nonylphenol