Dioxin detection based on immunoassay using a polyclonal antibody against octa-chlorinated dibenzo-p-dioxin (OCDD).
An enzyme-linked immunosorbent assay (ELISA) using a polyclonal antibody against octa-chlorinated dibenzo-p-dioxin (OCDD) is presented. This method is based on a competitive reaction between OCDD and OCDD-HRP (horseradish peroxidase) conjugate against the antibody, whereby OCDD-HRP is detected colorimetrically at 450 nm. The detection limit of OCDD was 0.78 pg mL(-1). Optimizing the reaction conditions of the assay, cross reactivities of some dioxins against the antibody are discussed.
Shimomura
M
M
Research Center for Advanced Science and Technology, The University of Tokyo, Japan.
Nomura
Y
Y
Lee
K H
KH
Ikebukuro
K
K
Karube
I
I
D000911:Antibodies, Monoclonal; D004785:Environmental Pollutants / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006728:Hormones / Q000032:analysis*; D000072317:Polychlorinated Dibenzodioxins / Q000031:analogs & derivatives* / Q000032:analysis* / Q000276:immunology
D000911:Antibodies, Monoclonal; D004785:Environmental Pollutants; D006728:Hormones; D000072317:Polychlorinated Dibenzodioxins; C018117:octachlorodibenzo-4-dioxin