title: Insulin-like growth factor I accelerates gastric ulcer healing by stimulating cell proliferation and by inhibiting gastric acid secretion.
abstract: BACKGROUND
  Given the importance of Insulin-Like Growth Factor I (IGF-I) to intestinal maintenance and the presence of IGF-I in salivary glands, we hypothesized that IGF-I participates in the healing of gastric ulcers. The aim of the study was to determine: 1) whether IGF-I applied locally would support gastric ulcer healing by increasing cell proliferation and 2) the effect of IGF-I on gastric acid secretion.
  
  
  METHODS
  Gastric ulcers were induced with a cryoprobe. Immediately thereafter, IGF-I (0.4, 4.0 and 40 microg) or vehicle was infiltrated perifocally. In another group, animals received a daily dose of 40 micromol omeprazole subcutaneously. Ulcer healing was evaluated by ulcer size and histological examination at 7 days. Pentagastrin-stimulated gastric acid secretion was evaluated in conscious rats with gastric fistula, after IGF-I (400 microg) had been injected intravenously.
  
  
  RESULTS
  IGF-I significantly reduced ulcer size, but only at low doses (0.4 microg/kg body weight (BW), P = 0.008; 4 microg/kg BW, P = 0.001). This effect was similar to omeprazole treatment. Histological examination after IGF-I administration showed increased cell proliferation, increased IGF-I content and down-regulated IGF-I receptors. The secretory studies demonstrated a significant decrease in gastric acid secretion 30 min after IGF-I bolus injection (IGF-I: 53 +/- 11 microEq; vehicle: 116 +/- 5 microEq; P=0.001), which lasted for more than 1 h.
  
  
  CONCLUSIONS
  IGF-I stimulates gastric ulcer healing, stimulating cell proliferation and inhibiting gastric acid secretion.
authors.affiliation: Dept. of General Surgery, University of Tübingen, Germany. Stephan.Coerper@t-Online.de
authors.forename: S
authors.initials: S
authors.lastname: Coerper
authors.forename: S
authors.initials: S
authors.lastname: Wolf
authors.forename: S
authors.initials: S
authors.lastname: von Kiparski
authors.forename: S
authors.initials: S
authors.lastname: Thomas
authors.forename: T T
authors.initials: TT
authors.lastname: Zittel
authors.forename: M B
authors.initials: MB
authors.lastname: Ranke
authors.forename: T K
authors.initials: TK
authors.lastname: Hunt
authors.forename: H D
authors.initials: HD
authors.lastname: Becker
mesh_terms: D000818:Animals; D002455:Cell Division / Q000187:drug effects; D005260:Female; D005744:Gastric Acid / Q000378:metabolism; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology / Q000502:physiology*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013276:Stomach Ulcer / Q000188:drug therapy* / Q000503:physiopathology; D014945:Wound Healing / Q000187:drug effects
chemical_list: D007334:Insulin-Like Growth Factor I