title: Identification of a secreted superoxide dismutase in Mycobacterium avium ssp. paratuberculosis.
abstract: Mycobacterium avium ssp. paratuberculosis (M. paratuberculosis), the causative agent of Johne's disease, is an important animal pathogen that has also been implicated in human disease. The major proteins expressed by M. paratuberculosis were analyzed by two-dimensional gel electrophoresis, and a superoxide dismutase (Sod) was identified from this protein profile. The M. paratuberculosis Sod has a molecular mass of 23 kDa and an isoelectric point of 6.1. Sequence analysis of the corresponding sodA gene from M. paratuberculosis indicates that this protein is a manganese-dependent enzyme. We show that the M. paratuberculosis Sod is actively secreted, suggesting that it may elicit a protective cellular immune response in the host during infection.
authors.affiliation: Department of Veterinary and Biomedical Sciences, University of Nebraska, Lincoln 68583-0905, USA.
authors.forename: X
authors.initials: X
authors.lastname: Liu
authors.forename: Z
authors.initials: Z
authors.lastname: Feng
authors.forename: N B
authors.initials: NB
authors.lastname: Harris
authors.forename: J D
authors.initials: JD
authors.lastname: Cirillo
authors.forename: H
authors.initials: H
authors.lastname: Bercovier
authors.forename: R G
authors.initials: RG
authors.lastname: Barletta
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D003001:Cloning, Molecular; D015180:Electrophoresis, Gel, Two-Dimensional; D005798:Genes, Bacterial; D006801:Humans; D008969:Molecular Sequence Data; D009162:Mycobacterium avium / Q000201:enzymology* / Q000235:genetics; D016927:Mycobacterium avium subsp. paratuberculosis / Q000201:enzymology* / Q000235:genetics; D010283:Paratuberculosis / Q000382:microbiology; D017386:Sequence Homology, Amino Acid; D013347:Subcellular Fractions / Q000201:enzymology; D013482:Superoxide Dismutase / Q000235:genetics* / Q000302:isolation & purification; D014376:Tuberculosis / Q000382:microbiology / Q000662:veterinary
chemical_list: D013482:Superoxide Dismutase