Expression analysis and chromosomal assignment of PRA1 and RILP genes.
PRA1 (prenylated Rab acceptor) is a general regulator of Rab proteins, while RILP (Rab interacting lysosomal protein) is a specific effector for Rab7. It has been shown that PRA1 interacts with Rab proteins and with VAMP2. Therefore PRA1 is probably an important factor for membrane traffic, linking together the function of Rab proteins and SNAREs. RILP has a key role in the control of transport to degradative compartments together with Rab7 and probably links Rab7 function to the cytoskeleton. Here we have studied by Northern blot the expression of the two genes in several different human tissues. The 0.8-kb mRNA for human PRA1 is ubiquitously expressed, while the two mRNAs for RILP are differentially expressed. In addition, we have assigned the human PRA1 gene to chromosome 19q13.13-q13.2 and the human RILP gene to chromosome 17p13.3.
Bucci
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Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Università degli Studi di Lecce, Via Monteroni, 73100 Lecce, Italy. cecilia.bucci@unile.it
De Gregorio
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Bruni
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D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000235:genetics*; D002874:Chromosome Mapping; D002886:Chromosomes, Human, Pair 17; D002888:Chromosomes, Human, Pair 19; D019204:GTP-Binding Proteins; D006801:Humans; D008565:Membrane Proteins; D012333:RNA, Messenger / Q000096:biosynthesis; D014018:Tissue Distribution; D033921:Vesicular Transport Proteins
D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; D008565:Membrane Proteins; C423317:RILP protein, human; D012333:RNA, Messenger; D033921:Vesicular Transport Proteins; D019204:GTP-Binding Proteins; C108941:RABAC1 protein, human