The expression of a barley HvNAS1 nicotianamine synthase gene promoter-gus fusion gene in transgenic tobacco is induced by Fe-deficiency in roots.
Nicotianamine (NA) is a precursor for mugineic acid-family phytosiderophores, which are a critical component of the Fe aquisition process in graminaceous plants. In addition, nicotianamine synthase (NAS) is strongly induced in these plants by Fe deficiency. NA is essential for Fe metabolism also in dicots, but NAS is not induced by Fe deficiency. We introduced a barley HvNAS1 promoter-gus fusion gene into tobacco. GUS activity was induced in the roots of these plants by Fe deficiency, and was constitutively expressed at a low level in their leaves.
Higuchi
K
K
Department of Applied Biological Chemistry, The University of Tokyo, Japan.
Tani
M
M
Nakanishi
H
H
Yoshiwara
T
T
Goto
F
F
Nishizawa
N K
NK
Mori
S
S
D019883:Alkyl and Aryl Transferases / Q000235:genetics*; D019677:Artificial Gene Fusion; D001483:Base Sequence; D018744:DNA, Plant / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D005966:Glucuronidase / Q000235:genetics / Q000378:metabolism; D001467:Hordeum / Q000201:enzymology* / Q000235:genetics*; D007501:Iron / Q000378:metabolism; D018517:Plant Roots / Q000378:metabolism; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D014026:Nicotiana / Q000201:enzymology / Q000235:genetics / Q000378:metabolism
D018744:DNA, Plant; D007501:Iron; D019883:Alkyl and Aryl Transferases; C118230:nicotianamine synthase; D005966:Glucuronidase