Hypothalamic neuroactivity in specific processes and central regulation of body temperature and water intake.
The method described was designed to elucidate the role of a particular neuronal system or specific nucleus in the central nervous system (CNS) in controlling physiological and biological functions. The neurochemical aspects of the CNS regulatory mechanism and related networks remain to be further investigated. There is little information available about the relationship between neuroactivity in the specific brain nuclei and physiological or biological responses in mammals. An adequate analysis of this relationship provides valuable insight to clarify which nucleus and what types of neurons are truly involved in the excitation of physiological events and its regulation. In the present study, we used microdialysis for stimulation of the anterior hypothalamus (AH) and simultaneous analysis of cholinergic activity, and we investigated c-Fos-like immunoreactivity (Fos-IR) in the brain in the same animal following microdialysis. The nuclear protein c-Fos, the product of c-fos oncogene, has been used as a marker of neuronal activity at the cellular level in the brain. Various physiological and pharmacological stimuli have been shown to induce Fos-IR in specific neuronal populations located in various regions of the brain. However, there are few studies investigating the responses produced by c-Fos expression in specific regions in same animals. We showed the involvement of hypothalamic cholinergic mechanisms in the thermoregulatory and water regulatory processes using the above procedures.
Takahashi
A
A
Department of Neuropsychopharmacology (Tsumura), Gunma University School of Medicine, Maebashi, 371, Gunma, Japan. takahashi_akira@mail.tsumura.co.jp
Ishimaru
H
H
Ikarashi
Y
Y
Kishi
E
E
Maruyama
Y
Y
D000109:Acetylcholine / Q000378:metabolism; D000818:Animals; D001831:Body Temperature / Q000187:drug effects / Q000502:physiology*; D001921:Brain / Q000378:metabolism; D018678:Cholinergic Agents / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004326:Drinking / Q000187:drug effects / Q000502:physiology*; D007032:Hypothalamus, Anterior / Q000187:drug effects / Q000502:physiology*; D008297:Male; D017551:Microdialysis; D009388:Neostigmine / Q000494:pharmacology; D009488:Neurosciences / Q000379:methods*; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
D018678:Cholinergic Agents; D016760:Proto-Oncogene Proteins c-fos; D009388:Neostigmine; D000109:Acetylcholine