Modulation of Chelidonii herba on GABA activated chloride current in rat PAG neurons.
Modulation of Chelidonii herba on gamma-aminobutyric acid (GABA) activated chloride current in the acutely dissociated periaqueductal gray (PAG) neuron was studied by nystatin-perforated patch-clamp technique. High concentrations of Chelidonii herba elicited ion current, that was blocked by bicuculline. Low concentrations reduced the GABA activated current in PAG. Two types of inhibitory action of Chelidonii herba on GABA activated current have been implicated in PAG. One is the inhibitory action of Chelidonii herba on GABA was abolished by naltrexone and the other is that of Chelidonii herba was potentiated by naltrexone. In addition, all of two types of action of Chelidonii herba are linked to pertussis toxin-sensitive GTP-binding proteins. These results suggest that the inhibitory modulation of Chelidonii herba on GABA activated current via G-proteins in PAG neuron is an important analgesic mechanism.
Kim
Y
Y
Department of Physiology, College of Medicine, Kyunghee University, Seoul, Korea.
Shin
M
M
Chung
J
J
Kim
E
E
Koo
G
G
Lee
C
C
Kim
C
C
D000818:Animals; D001640:Bicuculline / Q000494:pharmacology; D028281:Chelidonium; D018118:Chloride Channels / Q000037:antagonists & inhibitors / Q000187:drug effects*; D005260:Female; D018756:GABA Antagonists / Q000494:pharmacology; D008297:Male; D009271:Naltrexone / Q000494:pharmacology; D009292:Narcotic Antagonists / Q000494:pharmacology; D009474:Neurons / Q000187:drug effects*; D018408:Patch-Clamp Techniques; D010487:Periaqueductal Gray / Q000166:cytology / Q000187:drug effects*; D028321:Plant Preparations / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology*
D018118:Chloride Channels; D018756:GABA Antagonists; D009292:Narcotic Antagonists; D028321:Plant Preparations; D005680:gamma-Aminobutyric Acid; D009271:Naltrexone; D001640:Bicuculline