title: Regulation of intestinal apolipoprotein A-I synthesis by dietary phosphatidylcholine in newborn swine.
abstract: Phospholipid (PL) from both dietary sources and biliary secretions may be important in the regulation of intestinal apolipoprotein (apo) synthesis. We previously demonstrated the up-regulation of apo A-I secretion by phosphatidylcholine (PC) in a newborn piglet intestinal epithelial cell line. We hypothesized that dietary PC increases small intestinal apo A-I synthesis in vivo in the newborn piglet. Two-day-old female swine were fed by gavage for 48 h. Diets consisted of a formula containing 51% of calories as triacylglycerol providing 180 kcal/kg/24 h. The experimental group (+PC, n = 7) received 1 g/L added soybean PC, and the control group (-PC, n = 7) received no added PC. At the end of the study period, jejunal apo A-I, B, and A-IV synthesis was measured, and apo A-I mRNA levels were quantitated. Jejunal mucosal PL content and serum lipids and apo B and A-I levels were measured. Jejunal apo A-I synthesis was almost twice as high in the +PC group as compared to the -PC group with no difference in apo A-I mRNA levels. Jejunal content of PL was higher in the +PC group than in the -PC group. There were no differences in jejunal apo B and A-IV synthesis or serum levels of lipids and apo-lipoproteins between the two groups. Dietary PC supplementation in newborn swine up-regulated jejunal apo A-I synthesis. Apo A-IV synthesis, which is sensitive to fatty acid flux, was not significantly increased, which suggests a specific effect of PC on apo A-I synthesis. Lumenal PC may be important in the regulation of intestinal apo A-I synthesis in the neonate.
authors.affiliation: Arkansas Children's Hospital Research Institute, University of Arkansas for Medical Sciences, Little Rock 72202, USA.
authors.forename: H
authors.initials: H
authors.lastname: Wang
authors.forename: J
authors.initials: J
authors.lastname: Du
authors.forename: S
authors.initials: S
authors.lastname: Lu
authors.forename: Y
authors.initials: Y
authors.lastname: Yao
authors.forename: F
authors.initials: F
authors.lastname: Hunter
authors.forename: D D
authors.initials: DD
authors.lastname: Black
mesh_terms: D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism*; D016632:Apolipoprotein A-I / Q000096:biosynthesis* / Q000097:blood / Q000235:genetics; D001054:Apolipoproteins A / Q000096:biosynthesis; D001055:Apolipoproteins B / Q000096:biosynthesis / Q000097:blood; D008076:Cholesterol, HDL / Q000097:blood; D004041:Dietary Fats / Q000008:administration & dosage*; D002149:Energy Intake; D005260:Female; D007413:Intestinal Mucosa / Q000737:chemistry / Q000378:metabolism*; D007583:Jejunum / Q000737:chemistry / Q000378:metabolism; D010713:Phosphatidylcholines / Q000008:administration & dosage*; D010743:Phospholipids / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D013025:Glycine max / Q000737:chemistry; D013552:Swine; D014280:Triglycerides / Q000008:administration & dosage
chemical_list: D016632:Apolipoprotein A-I; D001054:Apolipoproteins A; D001055:Apolipoproteins B; D008076:Cholesterol, HDL; D004041:Dietary Fats; D010713:Phosphatidylcholines; D010743:Phospholipids; D012333:RNA, Messenger; D014280:Triglycerides; C022205:apolipoprotein A-IV