title: Intraspecific diversity of Oenococcus oeni isolated during red wine-making in Japan.
abstract: Using molecular and chemotaxonomic techniques, we studied the intraspecific diversity of Oenococcus oeni, a lactic acid bacterium isolated during red wine-making in Japan. The results confirmed high values of DNA-DNA relatedness and strong similarity among 16S rDNA sequences of the isolates with the O. oeni-type strain. Pulsed-field gel electrophoresis (PFGE) by NotI identified four patterns among the strains. Three different patterns of lactate dehydrogenase mobility were seen and there was a strong correlation between PFGE pattern and mobility. The present results suggest that the different strains of O. oeni comprise one species, and that variations in the genomic profiles of the different strains of O. oeni, including Japanese isolates are well correlated.
authors.affiliation: The Institute of Enology and Viticulture, Yamanashi University, Kofu, Japan. Yanagida@mail.yamanashi.ac.jp
authors.forename: H
authors.initials: H
authors.lastname: Sato
authors.forename: F
authors.initials: F
authors.lastname: Yanagida
authors.forename: T
authors.initials: T
authors.lastname: Shinohara
authors.forename: M
authors.initials: M
authors.lastname: Suzuki
authors.forename: K
authors.initials: K
authors.lastname: Suzuki
authors.forename: K
authors.initials: K
authors.lastname: Yokotsuka
mesh_terms: D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D005798:Genes, Bacterial / Q000235:genetics; D014644:Genetic Variation / Q000235:genetics*; D016680:Genome, Bacterial; D006095:Gram-Positive Cocci / Q000145:classification* / Q000201:enzymology / Q000235:genetics; D007564:Japan; D007770:L-Lactate Dehydrogenase / Q000737:chemistry; D007934:Leuconostoc / Q000145:classification / Q000201:enzymology / Q000235:genetics; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D014920:Wine / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D007770:L-Lactate Dehydrogenase