LC determination of carbamazepine in murine brain.
A reversed phase HPLC method for the determination of carbamazepine (CBZ) in the brain of adult mice is described. CBZ was recovered from murine brain by solvent-extraction with ethyl acetate and resolved from imipramine (internal standard) and brain endogenous material using a Lichrospher RP select B column with a linear gradient of acetonitrile (40-80 v/v, 25 min) in ammonium acetate buffer (25 mM, pH 4.0) with UV detection at 285 nm. The method is selective, reproducible and precise with a limit detection of 45 ng/ml and is suitable for the determination of CBZ in murine brain after intra-peritoneal administration.
Owen
A
A
Department of Pharmacology and Therapeutics, Ashton Street Medical School, The University of Liverpool, Liverpool L69 3GE, UK.
Tettey
J N
JN
Morgan
P
P
Pirmohamed
M
M
Park
B K
BK
D000818:Animals; D000927:Anticonvulsants / Q000378:metabolism*; D001921:Brain / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004049:Diethylcarbamazine / Q000378:metabolism*; D007274:Injections, Intraperitoneal; D008297:Male; D051379:Mice
D000927:Anticonvulsants; D004049:Diethylcarbamazine