Diverse developmental programs of Xenopus laevis metamorphosis are inhibited by a dominant negative thyroid hormone receptor.
Metamorphosis of anuran tadpoles is controlled by thyroid hormone (TH). Here we demonstrate that transgenic Xenopus laevis tadpoles expressing a dominant negative form of TH receptor-alpha are resistant to a wide variety of the metamorphic changes induced by TH. This result confirms that TH receptors mediate both early and late developmental programs of metamorphosis as diverse as growth in the brain, limb buds, nose and Meckel's cartilage, remodeling of the intestine, and death and resorption of the gills and tail.
Schreiber
A M
AM
Carnegie Institution of Washington, Department of Embryology, 115 West University Parkway, Baltimore, MD 21210, USA.
Das
B
B
Huang
H
H
Marsh-Armstrong
N
N
Brown
D D
DD
D000818:Animals; D030801:Animals, Genetically Modified; D016466:CHO Cells; D003094:Collagen / Q000235:genetics; D006224:Cricetinae; D003587:Cytomegalovirus / Q000235:genetics; D015870:Gene Expression; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000235:genetics; D008675:Metamorphosis, Biological; D011401:Promoter Regions, Genetic; D011988:Receptors, Thyroid Hormone / Q000235:genetics / Q000378:metabolism*; D013963:Thyroid Hormones / Q000378:metabolism; D019076:Transgenes; D014982:Xenopus laevis / Q000254:growth & development*
D008164:Luminescent Proteins; D011988:Receptors, Thyroid Hormone; D013963:Thyroid Hormones; D049452:Green Fluorescent Proteins; D003094:Collagen