Purification of saponin compounds in Bupleurum falcatum by solvent partitioning and preparative LC.
Saponin compounds (saikosaponin c, a, and d) in Bupleurum falcatum were partially purified by solvent partitioning of the herbal extract using diethyl ether, distilled water, n-butanol, and acetone. After separation of the saponins by preparative LC, the purity of each saikosaponin was more than 94%. The identities of purified individual saikosaponins were confirmed by TLC, analytical LC, and fast-atom bombardment mass spectrometry.
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Korea Food Research Institute, Songnam-si, Kyonggi-do, Republic of Korea. k9130sen@hanmail.net
Park
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D029972:Bupleurum / Q000737:chemistry*; D002853:Chromatography, Liquid; D015394:Molecular Structure; D009828:Oleanolic Acid / Q000031:analogs & derivatives*; D012502:Sapogenins / Q000737:chemistry / Q000302:isolation & purification*; D012503:Saponins / Q000302:isolation & purification*; D012997:Solvents
D012502:Sapogenins; D012503:Saponins; D012997:Solvents; D009828:Oleanolic Acid; C025759:saikosaponin D