title: Plant gene expression response to Agrobacterium tumefaciens.
abstract: To elucidate the nature of plant response to infection and transformation by Agrobacterium tumefaciens, we compared the cDNA-amplified fragment length polymorphism (AFLP) pattern of Agrobacterium- and mock-inoculated Ageratum conyzoides plant cell cultures. From 16,000 cDNA fragments analyzed, 251 (1.6%) were differentially regulated (0.5% down-regulated) 48 h after cocultivation with Agrobacterium. From 75 strongly regulated fragments, 56 were already regulated 24 h after cocultivation. Sequence similarities were obtained for 20 of these fragments, and reverse transcription-PCR analysis was carried out with seven to confirm their cDNA-AFLP differential pattern. Their sequence similarities suggest a role for these genes in signal perception, transduction, and plant defense. Reverse transcription-PCR analysis indicated that four genes involved in defense response are regulated in a similar manner by nonpathogenic bacteria, whereas one gene putatively involved in signal transduction appeared to respond more strongly to Agrobacterium. A nodulin-like gene was regulated only by Agrobacterium. These results demonstrate a rapid plant cell response to Agrobacterium infection, which overlaps a general response to bacteria but also has Agrobacterium-specific features.
authors.affiliation: Department of Botany, University of Washington, Seattle, WA 98195-5325, USA.
authors.forename: R F
authors.initials: RF
authors.lastname: Ditt
authors.forename: E W
authors.initials: EW
authors.lastname: Nester
authors.forename: L
authors.initials: L
authors.lastname: Comai
mesh_terms: D016960:Agrobacterium tumefaciens / Q000502:physiology*; D019659:Asteraceae / Q000235:genetics / Q000382:microbiology; D001483:Base Sequence; D002478:Cells, Cultured; D053938:DNA Fragmentation; D018744:DNA, Plant; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D008969:Molecular Sequence Data; D010947:Plants, Toxic; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D014026:Nicotiana / Q000235:genetics / Q000382:microbiology; D014170:Transformation, Genetic
chemical_list: D018744:DNA, Plant