Ultrastructural characterization of microvasculature in photoaging.
The cutaneous microvasculature was examined by electron microscopy in order to compare its characteristics in photodamaged preauricular skin and in sun-protected postauricular sites of 15 Japanese women aged 58-81 years. The characteristic ultrastructural features of the microvasculature in photodamaged skin compared with those in sun-protected skin included dilated vessels embedded in elastin which depressed endothelial cells, vessels surrounded by a thick amorphous material composed of multiple laminations of a basement membrane-like material, and activated endothelial cells which had increased numbers of cytoplasmic organelles and pinocytotic vesicles. A novel finding of this study in photodamaged vessels was an increased formation of new vessels (angiogenesis) via two distinct pathways. In severe elastosis, activated endothelial cells with densely packed intracytoplasmic microfilaments extended large pseudopods into the elastotic material. In contrast, isolated mesenchymal cells, which possessed immature Weibel-Palade bodies, were scattered around pre-existing vessels within the Grenz zone. In some cases, many mesenchymal cells with electron-lucent cytoplasms aggregated and interconnected by cytoplasmic processes, which was followed by the formation of vascular structures. These results suggest that there are significant ultrastructural differences in vessels between photoaged and intrinsically aged facial skin and that the photodamaged microvascular system is characterized by the co-existence of regressive changes and angiogenesis.
Toyoda
M
M
Department of Dermatology, Faculty of Medicine, Toyama Medical and Pharmaceutical University, 2630 Sugitani, 930-0194, Toyama, Japan. toyodam@toyoma-mpu.ac.jp
Nakamura
M
M
Luo
Y
Y
Morohashi
M
M
D000368:Aged; D000369:Aged, 80 and over; D044466:Asian People; D001485:Basement Membrane / Q000528:radiation effects / Q000648:ultrastructure; D001707:Biopsy, Needle; D003094:Collagen / Q000032:analysis; D004549:Elastin / Q000032:analysis; D004730:Endothelium, Vascular / Q000528:radiation effects* / Q000648:ultrastructure; D005260:Female; D006801:Humans; D007564:Japan; D008833:Microcirculation / Q000528:radiation effects* / Q000648:ultrastructure; D008875:Middle Aged; D015388:Organelles / Q000528:radiation effects / Q000648:ultrastructure; D012867:Skin / Q000098:blood supply* / Q000294:innervation / Q000528:radiation effects*; D015595:Skin Aging / Q000502:physiology / Q000528:radiation effects*; D013472:Sunlight / Q000009:adverse effects*
D003094:Collagen; D004549:Elastin