Effects of angiotensin II on the renal antioxidant activities of borderline hypertensive rats.
This study was aimed to study the angiotensin II (Ang II)-induced antioxidant changes in the kidney of borderline-hypertensive rats (BHR). We measured renal antioxidant enzyme activities, and glutathione (GSH) contents and lipid peroxide levels in relation to the age of subjects. In the antioxidant enzyme assays, consistent changes were not observed in relation to age. However, in the assay for reduced GSH, nonenzymatic antioxidant, contents of adult and aged rats were much greater than those of weanling rats. Subcutaneous injection of pressor dose of human Ang II (200 microg/kg over 90 min) significantly reduced enzymatic activities in the weanling (4-week-aged) and adult (10-week-aged) BHR. However, in the relatively aged (16-week-aged) rats, Ang II did not alter enzymatic activities. Renal GSH contents of aged BHR, were highly increased by Ang II. Renal lipid peroxide levels of weanling and adult BHR were increased by Ang II, but decreased in the aged rats. However, these characteristic changes of renal antioxidant due to Ang II of the BHR could not be observed in the age-matched control, Wistar-Kyoto rats (WKR). From these results, it can be concluded that impacts of oxidative stress on the kidney of BHR may be greater in the young rats.
Ha
J H
JH
Department of Pharmacology, Yeungnam University, Kyungsan, Republic of Korea.
Jin
D Q
DQ
Shin
S M
SM
Moon
H G
HG
Lee
E S
ES
Park
Y H
YH
D000375:Aging / Q000378:metabolism; D000804:Angiotensin II / Q000494:pharmacology*; D000818:Animals; D001794:Blood Pressure; D005260:Female; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D006973:Hypertension / Q000201:enzymology* / Q000503:physiopathology; D007279:Injections, Subcutaneous; D008054:Lipid Peroxides / Q000378:metabolism; D008297:Male; D010088:Oxidoreductases / Q000378:metabolism*; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D012016:Reference Values; D014662:Vasoconstrictor Agents / Q000494:pharmacology*; D014886:Weaning
D008054:Lipid Peroxides; D014662:Vasoconstrictor Agents; D000804:Angiotensin II; D010088:Oxidoreductases; D005978:Glutathione