title: Prognostic immunohistochemical markers of primary human melanomas.
abstract: BACKGROUND
  Several clinical and histological factors of primary melanomas comprise a relatively large quantity of prognostic information.
  
  
  OBJECTIVE
  To find immunohistochemical markers that can improve the prognostic accuracy achieved by factors that are available without extra laboratory work, i.e. mitotic rate, tumour thickness, ulceration, localization, gender and age.
  
  
  METHODS
  Immunohistochemical markers were determined on frozen sections. Univariate and multivariate Cox regression analyses were performed after 5-10 years follow-up.
  
  
  RESULTS
  Seven immunohistochemical markers were related to disease-free and overall survival in univariate Cox regression analysis: Ki-67, human leucocyte antigen (HLA) -DQ, HLA-DP, Muc 18, A-10-33, transferrin receptor, and H-2-8-10. Only Ki-67 (n = 399) and HLA-DQ (n = 452) retained prognostic significance when evaluated in multivariate analyses in several models together with tumour thickness alone and with tumour thickness, gender, mitotic rate, age, localization and ulceration.
  
  
  CONCLUSIONS
  Ki-67 and HLA-DQ may be useful for risk assessments in primary melanomas.
authors.affiliation: Fachklinik Hornheide, Dorbaumstrasse 300, D-48157 Münster, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Ostmeier
authors.forename: B
authors.initials: B
authors.lastname: Fuchs
authors.forename: F
authors.initials: F
authors.lastname: Otto
authors.forename: R
authors.initials: R
authors.lastname: Mawick
authors.forename: A
authors.initials: A
authors.lastname: Lippold
authors.forename: V
authors.initials: V
authors.lastname: Krieg
authors.forename: L
authors.initials: L
authors.lastname: Suter
mesh_terms: D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D015703:Antigens, CD; D014408:Biomarkers, Tumor / Q000378:metabolism*; D051929:CD146 Antigen; D002648:Child; D002675:Child, Preschool; D018572:Disease-Free Survival; D005260:Female; D005629:Frozen Sections; D006682:HLA-DP Antigens / Q000378:metabolism; D006683:HLA-DQ Antigens / Q000378:metabolism; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D019394:Ki-67 Antigen / Q000378:metabolism; D008297:Male; D008545:Melanoma / Q000378:metabolism* / Q000473:pathology; D008562:Membrane Glycoproteins / Q000378:metabolism; D008875:Middle Aged; D008940:Mitotic Index; D019006:Neural Cell Adhesion Molecules; D011379:Prognosis; D011990:Receptors, Transferrin / Q000378:metabolism; D012044:Regression Analysis; D012737:Sex Factors; D012878:Skin Neoplasms / Q000378:metabolism* / Q000473:pathology
chemical_list: D015703:Antigens, CD; D014408:Biomarkers, Tumor; D051929:CD146 Antigen; D006682:HLA-DP Antigens; D006683:HLA-DQ Antigens; D019394:Ki-67 Antigen; D008562:Membrane Glycoproteins; D019006:Neural Cell Adhesion Molecules; D011990:Receptors, Transferrin