title: Distribution and immunohistochemical characterisation of paracervical neurons innervating the oviduct in the pig.
abstract: The present study was aimed at disclosing the distribution of paracervical neurons projecting to the ampulla and isthmus of the porcine oviduct and the pattern(s) of co-existence of tyrosine hydroxylase (TH), dopamine beta-hydroxylase (D beta H), neuropeptide Y (NPY), substance P (SP), calcitonin gene-related peptide (CGRP) and nitric oxide synthase (NOS) within these nerve cell bodies. The fluorescent retrograde tracer Fast Blue (FB) was injected into the wall of the ampullar (n = 3) and isthmal (n = 3) part of the organ in six sexually immature female pigs. After a survival period of three weeks paracervical ganglia (PCG) were collected. 10 microns-thick cryostat sections of the ganglia were examined for the presence of FB-positive (FB+) nerve cells under the fluorescent microscope. Tracered neurons were counted in every third section and processed for double-labelling immunofluorescence according to the method of Wessendorf and Elde. 78.6% of FB+ neurons were projecting to the isthmus while 21.4% of the studied population innervated the ampulla of the oviduct. Double-labelling immunofluorescence revealed the existence of the following different chemically coded subpopulations of the studied perikarya: TH+/D beta H+, TH+/NPY+, TH+/NOS+, TH+/NOS-, SP-/NOS+, SP+/CGRP+.
authors.affiliation: Department of Animal Anatomy, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Bldg. 105 J., ul. Oczapowski 13, 10-917 Olsztyn-Kortowo II, Poland. czajak@moskit.uwm.edu.pl
authors.forename: K
authors.initials: K
authors.lastname: Czaja
authors.forename: K
authors.initials: K
authors.lastname: Wasowicz
authors.forename: M
authors.initials: M
authors.lastname: Klimczuk
authors.forename: P
authors.initials: P
authors.lastname: Podlasz
authors.forename: M
authors.initials: M
authors.lastname: Łakomy
mesh_terms: D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000032:analysis; D004299:Dopamine beta-Hydroxylase / Q000032:analysis; D005187:Fallopian Tubes / Q000294:innervation*; D005260:Female; D007001:Hypogastric Plexus / Q000737:chemistry / Q000166:cytology* / Q000201:enzymology; D007150:Immunohistochemistry; D009474:Neurons / Q000737:chemistry* / Q000201:enzymology*; D009478:Neuropeptide Y / Q000032:analysis; D019001:Nitric Oxide Synthase / Q000032:analysis; D013373:Substance P / Q000032:analysis; D013552:Swine; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis
chemical_list: D009478:Neuropeptide Y; D013373:Substance P; D019001:Nitric Oxide Synthase; D014446:Tyrosine 3-Monooxygenase; D004299:Dopamine beta-Hydroxylase; D015740:Calcitonin Gene-Related Peptide