title: Enhancement of the immune response in rabbits to a malaria DNA vaccine by immunization with a needle-free jet device.
abstract: We compared the needle free jet device device Biojector with syringe/needle as a method to administer a DNA vaccine encoding the Plasmodium falciparum circumsporozoite protein (PfCSP) in albino rabbits. A group of three rabbits was injected by the intramuscular (IM) route using a syringe/needle combination, a second group IM with the Biojector device and a third group both IM and intradermal (ID) using the Biojector. When animals were immunized with the Biojector IM or IM/ID as compared to the syringe/needle IM, we observed 10- and 50-fold greater antibody titers, as measured by enzyme immunoassay (EIA) and indirect fluorescence antibody test (IFAT), respectively. We also observed that the Biojector conferred a greater ability to prime the immune system as compared with the needle. The subsequent boosting of all animals with a recombinant canary pox virus (ALVAC) expressing PfCSP induced significantly higher titers in both Biojector groups of rabbits as compared with the needle and naive animals. These results provided the foundation for a clinical trial using the same regime.
authors.affiliation: Malaria Program, Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD 20910-7500, USA.
authors.forename: J C
authors.initials: JC
authors.lastname: Aguiar
authors.forename: R C
authors.initials: RC
authors.lastname: Hedstrom
authors.forename: W O
authors.initials: WO
authors.lastname: Rogers
authors.forename: Y
authors.initials: Y
authors.lastname: Charoenvit
authors.forename: J B
authors.initials: JB
authors.lastname: Sacci
authors.forename: D E
authors.initials: DE
authors.lastname: Lanar
authors.forename: V F
authors.initials: VF
authors.lastname: Majam
authors.forename: R R
authors.initials: RR
authors.lastname: Stout
authors.forename: S L
authors.initials: SL
authors.lastname: Hoffman
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000913:Antibodies, Protozoan / Q000096:biosynthesis* / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology; D028121:Canarypox virus / Q000235:genetics; D007271:Injections, Intradermal; D007273:Injections, Intramuscular; D007277:Injections, Jet; D017780:Malaria Vaccines / Q000008:administration & dosage* / Q000276:immunology; D008969:Molecular Sequence Data; D010963:Plasmodium falciparum / Q000276:immunology*; D015800:Protozoan Proteins / Q000235:genetics / Q000276:immunology*; D011817:Rabbits; D014611:Vaccination / Q000295:instrumentation*; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000276:immunology
chemical_list: D000913:Antibodies, Protozoan; D000914:Antibodies, Viral; D017780:Malaria Vaccines; D015800:Protozoan Proteins; D019444:Vaccines, DNA; C041595:circumsporozoite protein, Protozoan