Similar cellular effects induced by diesel exhaust particles from a representative diesel vehicle recovered from filters and Standard Reference Material 1650.
Standard reference diesel exhaust particles (DEP) SRM 1650 are often used to evaluate the toxicity of DEP. However, these particles did not necessarily reflect the effects of DEP representative of present diesel automobiles. This study was designed to compare the effects of SRM 1650 to DEP from representative cars (RC-DEP) on airway epithelial cells. Therefore we established a method to recover RC-DEP impacted on filters after emission from diesel automobiles on test beds. Electron microscopy and flow cytometry showed that these two types of particles were phagocytosed to the same extent by epithelial cells. This phagocytosis is not dependent on the adsorbed organic compounds in contrast to the cytotoxic effect evaluated by measurements of LDH release. This is emphasized by the fact that RC-DEP equipped with an oxidation catalyst are less cytotoxic than particles from a non-equipped vehicle or SRM 1650. This type of catalyst also reduces significantly the release of GM-CSF by bronchial epithelial cells. We have shown in the present paper that SRM 1650 may be used as a surrogate of DEP. However, exhaust gas post-treatment devices of current diesel automobiles reduce the cytotoxicity as well as the inflammatory response of these particles.
Boland
S
S
Laboratoire de Cytophysiologie et Toxicologie Cellulaire, Université Paris VII Denis Diderot, Tour 53/54 E3, case 70-73, 2 place Jussieu, 75251 Paris cédex 05, France. boland@paris7.jussieu.fr
Baeza-Squiban
A
A
Bonvallot
V
V
Houcine
O
O
Pain
C
C
Meyer
M
M
Marano
F
F
D000393:Air Pollutants / Q000737:chemistry / Q000633:toxicity*; D000818:Animals; D001980:Bronchi / Q000166:cytology / Q000187:drug effects; D002244:Carbon / Q000032:analysis / Q000633:toxicity; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D005374:Filtration; D005434:Flow Cytometry; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D010587:Phagocytosis / Q000187:drug effects / Q000502:physiology; D011817:Rabbits; D012015:Reference Standards; D014132:Trachea / Q000166:cytology / Q000187:drug effects; D001335:Vehicle Emissions / Q000032:analysis / Q000633:toxicity*
D000393:Air Pollutants; D001335:Vehicle Emissions; D002244:Carbon; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D007770:L-Lactate Dehydrogenase