The sequence of a 15 769 bp segment of Pichia anomala identifies the SEC61 and FBP1 genes and five new open reading frames.
We have determined the sequence of a 15 769 bp DNA segment of Pichia anomala. The sequence contains seven complete open reading frames (ORFs) longer than 100 amino acids and a putative tRNA gene. Two of the ORFs code for the well-characterized genes SEC61 (which codes for the core subunit of the ER translocation complex) and FBP1 (encoding fructose-1,6-bisphosphatase). A gene coding for a protein similar to S. cerevisiae YDL054c was found between the two genes. These three genes show a different organization (intermingled triples) in three yeast species: Saccharomyces cerevisiae, Candida albicans and P. anomala. Two out of the four remaining ORFs show weak homology with different proteins from other species and the other two show non-significant similarity with previously sequenced genes. The nucleotide sequence has been submitted to the EMBL database under Accession No. AJ306295.
Ruíz
T
T
Departamento de Microbiología y Biología Celular, Facultad de Farmacia, Universidad de La Laguna, 38071 La Laguna, Tenerife, Spain.
Sánchez
M
M
De la Rosa
J M
JM
Rodríguez
L
L
Domínguez
A
A
D000595:Amino Acid Sequence; D004271:DNA, Fungal; D006597:Fructose-Bisphosphatase / Q000737:chemistry / Q000235:genetics*; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics*; D010843:Pichia / Q000201:enzymology / Q000235:genetics*; D000069816:SEC Translocation Channels; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D004271:DNA, Fungal; D008565:Membrane Proteins; D000069816:SEC Translocation Channels; D006597:Fructose-Bisphosphatase