title: Inhibition of in vitro pre-mRNA splicing in S. cerevisiae by branched oligonucleotides.
abstract: A series of V- and Y-shaped nucleic acids, related to the splicing intermediates derived from S. cerevisiae actin pre-mRNA, were prepared. The effects of such branched nucleic acids (bNAs) on the efficiency of in vitro pre-mRNA splicing in yeast were studied. The exogenous bNAs each effect the efficiency of splicing, yet to different degrees, depending on the sugar composition and topology of the molecules. Y-shaped RNAs inhibited the formation of mRNA (i.e. RNA splicing) to the greatest extent.
authors.affiliation: Department of Chemistry, McGill University, Montreal, QC, Canada, H3A 2K6.
authors.forename: S
authors.initials: S
authors.lastname: Carriero
authors.forename: R S
authors.initials: RS
authors.lastname: Braich
authors.forename: R H
authors.initials: RH
authors.lastname: Hudson
authors.forename: D
authors.initials: D
authors.lastname: Anglin
authors.forename: J D
authors.initials: JD
authors.lastname: Friesen
authors.forename: M J
authors.initials: MJ
authors.lastname: Damha
mesh_terms: D009841:Oligonucleotides / Q000235:genetics / Q000494:pharmacology*; D012322:RNA Precursors / Q000037:antagonists & inhibitors* / Q000378:metabolism; D012326:RNA Splicing / Q000187:drug effects*; D012333:RNA, Messenger / Q000037:antagonists & inhibitors* / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism
chemical_list: D009841:Oligonucleotides; D012322:RNA Precursors; D012333:RNA, Messenger