A simple and sensitive detection system for Bacillus anthracis in meat and tissue.
OBJECTIVE
To detect and isolate Bacillus anthracis from meat and tissue by rapid and simple procedures.


RESULTS
Bacillus anthracis Pasteur II cells were added to 1 g lymph node and pig meat, which were then cut into small pieces and suspended in PBS. Aliquots were spread on Bacillus cereus selective agar (BCA) plates to isolate B. anthracis cells, and incubated in trypticase soy broth. The enrichment culture was used for nested PCR with B. anthracis specific primers, which were to confirm the presence of B. anthracis chromosomal DNA and the pXO1/pXO2 plasmids.


CONCLUSIONS
One cell of B. anthracis was detected by nested PCR from 1 g of the samples, and was also isolated on BCA plates according to colony morphology within two days.


CONCLUSIONS
These results could be useful for detecting animals with latent anthrax, and meat contaminated with B. anthracis, rapidly and simply.
Cheun
H I
HI
Department of Veterinary Microbiology, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.
Makino
S I
SI
Watarai
M
M
Shirahata
T
T
Uchida
I
I
Takeshi
K
K
D000818:Animals; D001408:Bacillus anthracis / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D001431:Bacteriological Techniques / Q000379:methods*; D003470:Culture Media; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D008198:Lymph Nodes / Q000382:microbiology*; D008460:Meat / Q000382:microbiology*; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D013552:Swine / Q000382:microbiology*; D013698:Templates, Genetic
D003470:Culture Media; D004269:DNA, Bacterial