Proteins of rat serum, urine, and cerebrospinal fluid: VI. Further protein identifications and interstrain comparison.
We have investigated the biological fluids--serum, cerebrospinal fluid, and urine--of three strains of rats; the present data extend our database (also available on-line) and may be of interest for pharmacological and toxicological investigation. Specifically, we have defined reference maps of the major protein components in cerebrospinal fluid and urine. Compartment-specific isoforms were recognized for transferrin and transthyretin. Mass spectrometric data established the cleavage site of the signal peptide and identified the N-terminal blocking group of prostaglandin D synthase from rat cerebrospinal fluid. A previously undescribed member of the family of low molecular mass rat urinary proteins was characterized as containing a sequence similar, but not identical, to the N-terminal region of rat urinary protein-2 (RUP-2), and divergent from RUP-1.
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Kennedy Institute of Rheumatology Division, Imperial College School of Medicine, Hammersmith, London, UK.
Gianazza
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Eberini
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Sironi
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Dunn
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MJ
Gemeiner
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Miller
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I
D000595:Amino Acid Sequence; D000818:Animals; D001798:Blood Proteins / Q000032:analysis*; D002556:Cerebrospinal Fluid Proteins / Q000032:analysis*; D030562:Databases, Protein; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D014644:Genetic Variation; D020407:Internet; D008297:Male; D008969:Molecular Sequence Data; D008970:Molecular Weight; D020033:Protein Isoforms / Q000032:analysis; D011506:Proteins / Q000032:analysis / Q000145:classification; D011507:Proteinuria / Q000652:urine; D051381:Rats / Q000097:blood / Q000134:cerebrospinal fluid / Q000378:metabolism* / Q000652:urine; D011917:Rats, Inbred Lew; D011921:Rats, Inbred WKY; D017207:Rats, Sprague-Dawley; D013045:Species Specificity; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014556:Urine / Q000737:chemistry*
D001798:Blood Proteins; D002556:Cerebrospinal Fluid Proteins; D020033:Protein Isoforms; D011506:Proteins; C035737:major urinary proteins