Genotyping the Prop-1 mutation in Ames dwarf mice.
The Ames dwarf mouse phenotype is based on a homozygous single gene mutation in the Prop-1 gene that markedly extends life span. Since its discovery, interest in breeding these mice as a model to study retardation of aging has increased dramatically. However, the homozygous Prop-1 mutants are infertile, which necessitates breeding heterozygotes. Heterozygotes cannot be distinguished from the wildtype, while the homozygote dwarf phenotype only becomes apparent after about 3 weeks. Hence, there is a need for a simple test to genotype individual animals at an early stage for the absence or presence of one or two copies of the Prop-1 mutant gene. Here we present a Prop-1 genotyping protocol, based on a PCR reaction followed by a PflMI digestion.
Dollé
M E
ME
Barshop Center for Longevity and Aging Studies, University of Texas Health Science Center at San Antonio, 15355 Lambda Drive-STCBM, San Antonio, TX 78245, USA. dolle@uthscsa.edu
Snyder
W K
WK
Vijg
J
J
D000818:Animals; D004392:Dwarfism / Q000235:genetics*; D005260:Female; D005838:Genotype; D018398:Homeodomain Proteins / Q000235:genetics*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D009154:Mutation; D014157:Transcription Factors / Q000235:genetics*
D018398:Homeodomain Proteins; C102134:Prophet of Pit-1 protein; D014157:Transcription Factors