Expression of the Pasteurella multocida ompH gene is negatively regulated by the Fur protein.
The fur gene of Pasteurella multocida has been cloned by complementation of an Escherichia coli fur mutant. The P. multocida fur gene, which encodes a predicted protein of 147 amino acids, displaying the highest identity (89%) with the same protein of Haemophilus influenzae, is negatively regulated by its own product. By construction of a P. multocida fur mutant, it has been demonstrated that the ompH gene, encoding a major structural protein of the outer membrane, presenting high antigenicity power, is negatively regulated by iron and glucose. Furthermore, wild-type and fur-defective cells of P. multocida show the same level of virulence.
Bosch
M
M
Department of Genetics and Microbiology, Universitat Autònoma de Barcelona (UAB), Bellaterra, 08193 Barcelona, Spain.
Tarragó
R
R
Garrido
M E
ME
Campoy
S
S
Fernández de Henestrosa
A R
AR
Pérez de Rozas
A M
AM
Badiola
I
I
Barbé
J
J
D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D015536:Down-Regulation; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D005947:Glucose / Q000494:pharmacology; D007501:Iron / Q000494:pharmacology; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D016979:Pasteurella multocida / Q000235:genetics* / Q000472:pathogenicity; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D014774:Virulence
D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D012097:Repressor Proteins; C068477:ferric uptake regulating proteins, bacterial; C067128:ompH protein, bacteria; D007501:Iron; D005947:Glucose