title: Novel propargylamine-linked nucleosides for high throughput SNP genotyping by MALDI-TOF MS.
abstract: The synthesis of positively charged and mass tagged nucleosides containing a quaternary ammonium functionality within the penultimate position of a primer is described. Neutralization of the sugar/thiophosphate backbone by alkylation increases the detection sensitivity in the mass spectrometric analysis by a factor of at least 100. The variable introduction of these novel compounds within the extension primers enables flexible design of multiplex genotyping reactions.
authors.affiliation: Bruker Saxonia Analytik GmbH, Permoserstr. 15, D-04318 Leipzig, Germany.
authors.forename: T
authors.initials: T
authors.lastname: Wenzel
authors.forename: T
authors.initials: T
authors.lastname: Fröhlich
authors.forename: K
authors.initials: K
authors.lastname: Strassburger
authors.forename: S
authors.initials: S
authors.lastname: Richter
authors.forename: J
authors.initials: J
authors.lastname: Bimmler
authors.forename: C
authors.initials: C
authors.lastname: Franke
authors.forename: I
authors.initials: I
authors.lastname: Thomas
authors.forename: M
authors.initials: M
authors.lastname: Kostrzewa
mesh_terms: D004247:DNA / Q000737:chemistry / Q000235:genetics; D005838:Genotype; D006801:Humans; D009705:Nucleosides / Q000138:chemical synthesis* / Q000737:chemistry; D009943:Organophosphorus Compounds / Q000737:chemistry; D010293:Pargyline / Q000031:analogs & derivatives* / Q000737:chemistry*; D020641:Polymorphism, Single Nucleotide; D011437:Propylamines / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
chemical_list: D009705:Nucleosides; D009943:Organophosphorus Compounds; D011437:Propylamines; C434331:phosphoramidite; C009054:propargylamine; D004247:DNA; D010293:Pargyline