title: Efficient replication of caprine arthritis-encephalitis virus in goat granulosa cells.
abstract: Recent reports demonstrated the susceptibility of epithelial cells from different organs to caprine arthritis-encephalitis virus (CAEV) both in vitro and in vivo. Since granulosa cells (GC) are of epithelial origin and currently used for in vitro oocyte maturation, we addressed the question whether these cells are susceptible or resistant to CAEV infection. GC were isolated from goats from certified CAEV-free herds. PCR analysis on GC DNA using CAEV specific primers confirmed the absence of CAEV infection and immunocytochemistry using specific K813 anti-cytokeratin monoclonal antibodies confirmed the epithelial nature of GC. These cells were then inoculated with CAEV using two strains: the CAEV-pBSCA molecular clone and the CAEV-3112 French field isolate. Cytopathic effects (CPE) were observed on cell culture monolayers inoculated with both CAEV strains. Expression of CAEV proteins was shown both by immunocytochemistry using anti-p24 gag specific antibodies and by immunoprecipitation using an hyperimmune serum. Supernatant of infected cells were shown to contain high titers (ranging 10(5) tissue culture infectious doses 50 per ml: TCID(50) per ml) of infectious cytopathic viruses when assayed onto the indicator goat synovial membrane (GSM) cells. Our findings demonstrate the large cell tropism of CAEV and suggest that GC could serve as a reservoir for the virus during the sub-clinical phase of infection. Furthermore, given the high seroprevalence of CAEV in the all industrialised countries and the large number of ovaries derived from unknown serological status animals used for in vitro goat embryo production, one can conclude that these feeder cell cultures might be a potential source of early transmission of CAEV to goat embryos.
authors.affiliation: Laboratoire d'Etude des Risques Sanitaires liés aux Biotechnologies de la Reproduction, Ecole Nationale Vétérinaire de Nantes, Atlanpole-La Chantrerie, B.P. 40706 44307, Cedex, Nantes, France. lamara@vet-nantes.fr
authors.forename: A
authors.initials: A
authors.lastname: Lamara
authors.forename: F
authors.initials: F
authors.lastname: Fieni
authors.forename: L
authors.initials: L
authors.lastname: Mselli-Lakhal
authors.forename: D
authors.initials: D
authors.lastname: Tainturier
authors.forename: Y
authors.initials: Y
authors.lastname: Chebloune
mesh_terms: D000818:Animals; D016088:Arthritis-Encephalitis Virus, Caprine / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D002478:Cells, Cultured; D005260:Female; D006041:Goats; D006107:Granulosa Cells / Q000166:cytology / Q000821:virology*; D014779:Virus Replication