Morphological and molecular changes of maize plants after seeds been flown on recoverable satellite.
Dry seeds of Zea mays, heterozygous for Lw1/lw1 alleles, sandwiched between nuclear track detectors aboard Chinese satellite for 15 days, were recovered and mutations in morphological characters on plants developed from these seeds, as well as their selected progenies, were investigated. The dosimetric results indicated that 85% of the seeds received at least 1 hit with Z > or =  20. About 10% of plants developed from flown seeds and 40% of observed selfed lines from the first generation plants showed some morphological changes, such as yellow stripes displayed on leaves, dwarf, anomogensis of floral organs and yellow-green seedlings, when compared with those from ground control. Using yellow stripes on leaves as the main endpoint for evaluating mutation induced in space environment, the frequency of stripe occurrence was 4.6% in the first generation plants, comparable with the results obtained from Long Duration Exposure Facility (LDEF) mission (Mei et al., 1994), but much lower than those from ground based 60 Co-gamma treatment at a dose of 100 Gy, which reached 35.5% in the selfed lines of the second generation. One hundred and ten random primers were screened in RAPD analysis to detect the variation on genomic DNA of plants with stripes on leaves. Of these primers, 10.9% were able to generate polymorphic bands between mutated plants and control, also, common band patterns in several progenies with the same mutation phenotype were observed. These results demonstrated that space radiation environment could induce inheritable mutagenic effects on plant seeds, and verified the change in genetic material in the mutants. Further study will be needed for a better understand of the nature and mechanism of this induction of mutation.
Mei
M
M
South China Agricultural University, Guangzhou, China.
Qiu
Y
Y
Sun
Y
Y
Huang
R
R
Yao
J
J
Zhang
Q
Q
Hong
M
M
Ye
J
J
D003037:Cobalt Radioisotopes; D003359:Cosmic Radiation; D018744:DNA, Plant / Q000528:radiation effects*; D005720:Gamma Rays; D020450:Heavy Ions; D018499:Linear Energy Transfer; D016296:Mutagenesis; D009154:Mutation; D011829:Radiation Dosage; D019105:Random Amplified Polymorphic DNA Technique; D012639:Seeds / Q000235:genetics / Q000254:growth & development / Q000528:radiation effects*; D013026:Space Flight; D014893:Weightlessness; D003313:Zea mays / Q000235:genetics / Q000254:growth & development / Q000528:radiation effects
D003037:Cobalt Radioisotopes; D018744:DNA, Plant