title: Lymphocyte collection for donor leucocyte infusion from normal donors: estimation of the minimum processed blood volume and safety of the procedure.
abstract: OBJECTIVE
  To estimate the minimum volume of processed blood necessary for the purpose of donor leucocyte infusion (DLI), we determined the number of CD3+ cells harvested by apheresis from normal donors and examined adverse events during the procedure.
  
  
  METHODS
  Leukapheresis utilizing the COBE Spectra' was performed a total of 24 times from 12 normal donors. Blood counts were obtained and the number of CD3+ cells was evaluated before and after apheresis and in the peripheral blood products. Complications associated with the procedure were documented.
  
  
  RESULTS
  Blood products contained, on average, 51.1% CD3+ cells. A linear correlation was found between the number of CD3+ cells collected and the processed blood volume, up to 12 000 ml (r = 0.930, P < 0.001). Cytoreduction was observed in all donors after apheresis. In particular, the platelet and lymphocyte values decreased to approximately 70% of the preapheresis value. Two donors reported adverse effects: one was a mild vaso-vagal reaction and the other was citrate-related lip paresthesia. These symptoms were mild and disappeared spontaneously.
  
  
  CONCLUSIONS
  We found a linear correlation between the number of CD3+ cells collected and the processed blood volume, which enabled us to estimate the minimum volume of processed blood necessary for DLI. The adverse events observed were acceptable.
authors.affiliation: Division of Transfusion Medicine, Kanazawa University Hospital, Kanazawa, Ishikawa, Japan. hsatoh@med.kanazawa-u.ac.jp
authors.forename: H
authors.initials: H
authors.lastname: Sato
authors.forename: S
authors.initials: S
authors.lastname: Shiobara
authors.forename: S
authors.initials: S
authors.lastname: Yasue
authors.forename: T
authors.initials: T
authors.lastname: Chuhjo
authors.forename: S
authors.initials: S
authors.lastname: Nakao
mesh_terms: D000293:Adolescent; D000328:Adult; D001772:Blood Cell Count; D001782:Blood Donors; D001810:Blood Volume; D017252:CD3 Complex / Q000032:analysis; D005260:Female; D006801:Humans; D007937:Leukapheresis / Q000295:instrumentation / Q000379:methods / Q000592:standards*; D017710:Lymphocyte Transfusion; D008297:Male; D008875:Middle Aged; D012044:Regression Analysis
chemical_list: D017252:CD3 Complex