title: Protein crystallization in space.
abstract: The microgravity environment of space is an ideal place to study the complicated protein crystallization process and to grow good-quality protein crystals. A series of crystal growth experiments of 10 different proteins was carried out in space on a Chinese re-entry satellite FSW-2 in August, 1992. The experiments were performed for about two weeks at a temperature of 18.5 +/- 0.5 degrees C using a tube-like crystallization apparatus made in the Shanghai Institute of Technical Physics, Academia Sinica. More than half of 48 samples from 6 proteins produced crystals, and the effects of microgravity on protein crystal growth were observed, especially for hen-egg white lysozyme and an acidic phospholipase A2 from the venom of Agkistrodon halys Pallas. Analyses of the crystallization of these two enzymes in this mission showed that the microgravity environment in space may be beneficial to improve size, external perfection, morphology, internal order, and nucleation of protein crystals. Some of these positive microgravity effects were also demonstrated by the growth of protein crystals in gelled solution with the above two enzymes. A structural analysis of the tetragonal lysozyme crystal grown in space is in progress.
authors.affiliation: Institute of Biophysics, Academia Sinica, Beijing, PR China.
authors.forename: R C
authors.initials: RC
authors.lastname: Bi
authors.forename: L L
authors.initials: LL
authors.lastname: Gui
authors.forename: Q
authors.initials: Q
authors.lastname: Han
authors.forename: F L
authors.initials: FL
authors.lastname: Shen
authors.forename: K
authors.initials: K
authors.lastname: Shi
authors.forename: Y P
authors.initials: YP
authors.lastname: Wang
authors.forename: S Z
authors.initials: SZ
authors.lastname: Chen
authors.forename: Y L
authors.initials: YL
authors.lastname: Hu
authors.forename: X T
authors.initials: XT
authors.lastname: Niu
authors.forename: J
authors.initials: J
authors.lastname: Dong
authors.forename: Y C
authors.initials: YC
authors.lastname: Zhou
authors.forename: N Q
authors.initials: NQ
authors.lastname: Lin
mesh_terms: D000721:Ancrod / Q000737:chemistry; D000818:Animals; D003460:Crystallization; D004527:Egg Proteins / Q000737:chemistry; D009113:Muramidase / Q000737:chemistry*; D010740:Phospholipases / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D013026:Space Flight / Q000295:instrumentation*; D014893:Weightlessness
chemical_list: D004527:Egg Proteins; D011506:Proteins; D010740:Phospholipases; D009113:Muramidase; D000721:Ancrod