Fluorescence resonance energy transfer dye nucleotide terminators: a new synthetic approach for high-throughout DNA sequencing.
Fluorescence resonance energy transfer (FRET) based dye-nucleotide terminators (10-13) were designed, synthesized, and formulated with Thermo Sequenase II DNA polymerase into a robust kit for high throughput DNA sequencing. The key energy transfer (ET) rigid and linear linker (2), required for the syntheses of energy transfer cassettes (6-9) was synthesized via Heck coupling reaction on t-Boc-L-4-iodo-phenylalanine (1) with N-TFA-propargylamine.
Nampalli
S
S
Amersham Pharmacia Biotech, Piscataway, New Jersey 08855, USA.
Khot
M
M
Nelson
J R
JR
Flick
P K
PK
Fuller
C W
CW
Kumar
S
S
D001483:Base Sequence; D004247:DNA / Q000032:analysis; D004735:Energy Transfer; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000138:chemical synthesis*; D008969:Molecular Sequence Data; D009711:Nucleotides / Q000138:chemical synthesis*; D017422:Sequence Analysis, DNA / Q000379:methods*; D013050:Spectrometry, Fluorescence / Q000379:methods
D005456:Fluorescent Dyes; D009711:Nucleotides; D004247:DNA