alpha1-acid glycoprotein possesses in vitro pro- and antiinflammatory activities.
We studied the effects of alpha1-acid glycoprotein on tumor necrosis factor-alpha (TNF-alpha) and interleukin-10 (IL-10) production and lymphocyte response to phytohemagglutinin in cultured peripheral blood mononuclear leukocytes from 6 healthy donors. We observed 2 opposite responses to alpha1-acid glycoprotein: first, stimulation of TNF-alpha and IL-10 production and inhibition of lymphocyte proliferation, and second, suppression of cytokine production and stimulation of lymphocyte proliferation. In cell cultures isolated from 4 of 6 donors, the TNF-alpha/IL-10 ratio remained unchanged after addition of native alpha1-acid glycoprotein, but some fractions isolated by chromatography on concanavalin A-Sepharose changed this parameter. These changes were most pronounced after treatment with fraction C enriched with molecules with incomplete (biantennary) carbohydrate chains. The mechanisms of alpha1-acid glycoprotein-induced effects on peripheral blood mononuclear leukocytes are discussed.
Pukhal'skii
A L
AL
Methodical-and-Genetic Research Center, Russian Academy of Medical Sciences, Moscow. vikgor@arstel.ru
Shmarina
G V
GV
Lyutov
A G
AG
Novikova
L I
LI
Shemyakin
I G
IG
Aleshkin
V A
VA
D002455:Cell Division; D002478:Cells, Cultured; D003208:Concanavalin A / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007249:Inflammation / Q000378:metabolism*; D016753:Interleukin-10 / Q000096:biosynthesis; D007962:Leukocytes / Q000378:metabolism; D008954:Models, Biological; D009961:Orosomucoid / Q000378:metabolism*; D010835:Phytohemagglutinins / Q000378:metabolism; D012685:Sepharose / Q000737:chemistry; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000378:metabolism
D009961:Orosomucoid; D010835:Phytohemagglutinins; D014409:Tumor Necrosis Factor-alpha; D003208:Concanavalin A; D016753:Interleukin-10; D012685:Sepharose