title: The djlA gene acts synergistically with dnaJ in promoting Escherichia coli growth.
abstract: The DnaK chaperone of Escherichia coli is known to interact with the J domains of DnaJ, CbpA, and DjlA. By constructing multiple mutants, we found that the djlA gene was essential for bacterial growth above 37 degrees C in the absence of dnaJ. This essentiality depended upon the J domain of DjlA but not upon the normal membrane location of DjlA.
authors.affiliation: Département de Biochimie Médicale, Centre Médical Universitaire, Université de Genève, 1211 Genève 4, Switzerland. pierre.genevaux@medecine.unige.ch
authors.forename: P
authors.initials: P
authors.lastname: Genevaux
authors.forename: F
authors.initials: F
authors.lastname: Schwager
authors.forename: C
authors.initials: C
authors.lastname: Georgopoulos
authors.forename: W L
authors.initials: WL
authors.lastname: Kelley
mesh_terms: D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D029968:Escherichia coli Proteins; D050956:HSP40 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000235:genetics* / Q000378:metabolism*; D006358:Hot Temperature; D009154:Mutation
chemical_list: C085674:CbpA protein, E coli; D004268:DNA-Binding Proteins; C494634:DjlA protein, E coli; C494635:DnaJ protein, E coli; D029968:Escherichia coli Proteins; D050956:HSP40 Heat-Shock Proteins; D006360:Heat-Shock Proteins