Eicosapentaenoic acid modulates cyclin expression and arrests cell cycle progression in human leukemic K-562 cells.
Eicosapentaenoic acid (EPA) is a polyunsaturated fatty acid (20:5omega3) found primarily in aquatic organisms. We have shown previously that EPA inhibits the growth but is not toxic to human leukemic K-562 cells. In this study, the anti-proliferative effect of EPA on the leukemic cells was further determined and its impacts on cell cycle progression and cyclin expression were investigated. EPA inhibited proliferation of K-562 cells, which was associated with accumulation of G0/G1 cells and down-regulation of cyclin E expression. Cyclin B1-expressing cells were also reduced showing that down-regulation of cyclin expression might be important in the anti-proliferation of EPA.
Chiu
L C
LC
Department of Biology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, P.R. China. chimingchiu@cuhk.edu.hk
Ooi
V E
VE
Wan
J M
JM
D017209:Apoptosis / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects*; D019926:Cyclin B / Q000378:metabolism*; D056744:Cyclin B1; D019927:Cyclin E / Q000378:metabolism*; D015536:Down-Regulation; D015118:Eicosapentaenoic Acid / Q000494:pharmacology*; D005434:Flow Cytometry; D016193:G1 Phase / Q000187:drug effects; D006801:Humans; D020014:K562 Cells / Q000187:drug effects* / Q000378:metabolism; D016192:Resting Phase, Cell Cycle / Q000187:drug effects
C534699:CCNB1 protein, human; D019926:Cyclin B; D056744:Cyclin B1; D019927:Cyclin E; D015118:Eicosapentaenoic Acid