The influence of short term gravity changes on mouse spleen lymphocytes in vitro (microfluorimetry with probes).
Despite of the great successes achieved in gravitational cell biology, problem of mechanisms underlying cell effects of gravity is far from being solved. Available data  are very contradictory, because of the differences in experimental conditions: gravity value, exposition time, type of cells and other factors. Besides, there are no satisfactory methods to avoid additional influence of various biologically active factors on cells during the space experiments. It is absolutely clear, that successful development in this field may be provided only by investigations in controlled experimental conditions, including analysis of cell functional activity and state of cell regulatory systems. One of the most adequate approaches for these scientific tasks is cell microfluorimetry with using of fluorescent probes. The study of influence of short-term pattern changes in gravity (clinostation and centrifugation) on hydrolytic activity of intracellular content of mouse spleen lymphocytes, leakage of accumulated fluoresce in and intracellular pH is the goal of present research.
Turovetsky
V B
VB
Moscow State University, Russia.
Andreev
A I
AI
Buravkova
L B
LB
Rubin
A B
AB
D000818:Animals; D002498:Centrifugation; D003287:Contrast Media / Q000378:metabolism; D004950:Esterases / Q000378:metabolism; D019793:Fluorescein / Q000378:metabolism; D006112:Gravitation; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D018471:Hypergravity; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000378:metabolism*; D051379:Mice; D012399:Rotation; D013154:Spleen / Q000166:cytology*; D018474:Weightlessness Simulation
D003287:Contrast Media; D004950:Esterases; D019793:Fluorescein