The use of centrifugation to study early Drosophila embryogenesis.
By the end of 10th nuclear cycle, the somatic nuclei of the Drosophila embryo have migrated to the periphery of the egg.  Centrifugation of embryos did not result in the displacement of these nuclei, since cytoskeletal elements anchor them to the cortex.  But, mild centrifugal forces displace the centrally located, nascent yolk nuclei.  If this increased sensitivity to hypergravity occurs before the beginning of nuclear differentiation during cycle 8, when the nascent yolk and somatic nuclei physically separate, then it would mark the earliest functional difference between these two lineages.
Abbott
M K
MK
Division of Biology, NSCORT, Kansas State University, Manhattan 66506, USA.
D000818:Animals; D002467:Cell Nucleus / Q000502:physiology; D016764:Cell Polarity / Q000502:physiology*; D002498:Centrifugation; D018385:Centrosome / Q000502:physiology; D004331:Drosophila melanogaster / Q000196:embryology*; D004625:Embryo, Nonmammalian / Q000502:physiology; D018471:Hypergravity