title: Changes in bone noncollagenous proteins and bone mineral loss in lumbar vertebrae of tail-suspended rats.
abstract: The purpose of the study is to clarify whether the noncollagenous proteins play a role in bone mineral loss and whether the quality of osteocalcin (OC) is deteriorated in simulated weightlessness. Noncollagenous proteins, such as OC and albumin, have been shown to be effective in promoting bone mineralization. In this study, the contents of OC, albumin, and mineral in the third lumbar vertebra (L3) were determined in rats suspended by tail for 21 days. The concentration of serum-immunoreactive osteocalcin (irOC) were also measured before and after adsorption to hydroxylapatite. The results showed that the contents of mineral, osteocalcin, and albumin in L3 were significantly decreased in suspended rats. The concentration of serum irOC and serum irOC with a high affinity to hydroxylapatite (irOCbound) were significantly decreased, but the low affinity (irOCfree) remained unaffected. The data suggest that the mineral loss in L3 of suspended rats is related to the decreased contents of osteocalcin, and albumin in bone mass. The incomplete gamma-carboxylation of osteocalcin in suspended rats is also one of the important causes of bone loss.
authors.affiliation: Institute of Space Medico-Engineering, Beijing, China.
authors.forename: W
authors.initials: W
authors.lastname: Cui
authors.forename: Z
authors.initials: Z
authors.lastname: Shi
authors.forename: C
authors.initials: C
authors.lastname: Liu
authors.forename: Q
authors.initials: Q
authors.lastname: Zheng
mesh_terms: D000418:Albumins / Q000378:metabolism*; D000818:Animals; D018488:Bone Demineralization, Pathologic / Q000209:etiology / Q000503:physiopathology*; D015519:Bone Density / Q000502:physiology; D003653:Decarboxylation; D019417:Hindlimb Suspension / Q000009:adverse effects*; D008159:Lumbar Vertebrae / Q000378:metabolism*; D008297:Male; D015675:Osteocalcin / Q000097:blood / Q000737:chemistry* / Q000145:classification / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D018474:Weightlessness Simulation
chemical_list: D000418:Albumins; D015675:Osteocalcin