Drosophila mitochondrial transcription factor A: characterization of its cDNA and expression pattern during development.
We cloned a cDNA for Drosophila mitochondrial transcription factor A (D-mtTFA) and characterized the recombinant protein. In Drosophila Kc cells, D-mtTFA was localized in the mitochondria, but not in the nucleus. By repetitive precipitation with His-tag and PCR amplification, the consensus nucleotide sequence for D-mtTFA-binding was determined to be 5'-TTATC/G. The binding sequence was found to be clustered in the A + T region of mitochondrial DNA which is suggested to be a replication origin and promoter region for light strand and heavy strand. We found a DNA replication-related element (DRE)-like sequence located upstream of the transcription initiation site of the D-mtTFA gene and obtained results indicating that DRE-binding factor (DREF) can bind to the DRE-like sequence of the D-mtTFA gene. The data suggest that transcription of the D-mtTFA gene is under control of the DRE/DREF regulatory system. Based on these results, the functions of D-mtTFA were discussed in relation to mitochondrial biogenesis of Drosophila melanogaster.
Takata
K
K
Department of Applied Biological Science, Faculty of Science and Technology, Science University of Tokyo, 2641 Yamazaki, Noda-shi, Chiba-ken 278-8510, Japan.
Yoshida
H
H
Hirose
F
F
Yamaguchi
M
M
Kai
M
M
Oshige
M
M
Sakimoto
I
I
Koiwai
O
O
Sakaguchi
K
K
D000818:Animals; D001483:Base Sequence; D002455:Cell Division; D002478:Cells, Cultured; D003001:Cloning, Molecular; D016384:Consensus Sequence; D018076:DNA, Complementary / Q000032:analysis; D004268:DNA-Binding Proteins; D004331:Drosophila melanogaster / Q000235:genetics; D005786:Gene Expression Regulation; D006801:Humans; D008928:Mitochondria / Q000235:genetics*; D024101:Mitochondrial Proteins; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009687:Nuclear Proteins / Q000145:classification / Q000235:genetics*; D013347:Subcellular Fractions; D014157:Transcription Factors / Q000145:classification / Q000235:genetics*
D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D024101:Mitochondrial Proteins; D009687:Nuclear Proteins; C499631:TFAM protein, human; D014157:Transcription Factors; C422562:mitochondrial transcription factor A