[Fluorescent analysis of irradiation-induced changes in chromatin state].
A fluorescent technique with Hoechst-33258 and acridine orange staining was used to assess changes in chromatin state induced by radiation. Fluorochromes with different modes of binding to DNA were chosen. In T lymphocytes chronic irradiation caused a rearrangement of binding between nonhiston proteins and lipids accompanied by conformational changes in DNA, resulting in chromatin condensation. The decrease in fluorescence probably resulted from a reduction in the number of sites accessible for dye binding. After acute irradiation, the fluorescence intensity decreases predominantly due to double-strand breaks.
Gordon
R Ia
RIa
Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, 142290 Russia.
Novoselova
E G
EG
Kruglova
N L
NL
Mel'nikova
E V
EV
Khutsian
S S
SS
D000818:Animals; D006690:Bisbenzimidazole; D002843:Chromatin / Q000378:metabolism / Q000528:radiation effects*; D004247:DNA / Q000737:chemistry / Q000528:radiation effects; D004249:DNA Damage; D005456:Fluorescent Dyes; D005720:Gamma Rays; D008297:Male; D015530:Nuclear Matrix / Q000528:radiation effects; D051381:Rats; D017208:Rats, Wistar; D013154:Spleen / Q000737:chemistry / Q000166:cytology / Q000528:radiation effects; D013601:T-Lymphocytes / Q000378:metabolism / Q000528:radiation effects
D002843:Chromatin; D005456:Fluorescent Dyes; D004247:DNA; D006690:Bisbenzimidazole