Comparative study of catalytic (DNA-hydrolyzing) and cytotoxic properties of anti-dna autoantibodies.
DNA-hydrolyzing and cytotoxic properties of anti-DNA autoantibodies isolated from patients with systemic autoimmune diseases and from autoimmune MRL-lpr/lpr, SJL/J, and (NZBx NZW)F1 mice were studied. Cytotoxic and catalytic properties of these antibodies correlated. A relationship between the stage of systemic lupus erythematosus and catalytic and cytotoxic properties of DNA abzymes was revealed. Of all studied cells, L929 cells were most sensitive were most sensitive to in vitro effect of antibodies. Treatment of target cells with anti-DNA autoantibodies with cytotoxic properties induced internucleosomal DNA fragmentation, which is characteristic of apoptosis.
Suchkov
S V
SV
Laboratory of Clinical Immunology, Moscow Regional Research and Clinical Institute, Moscow. grei@ibch.ru
D000818:Animals; D000974:Antibodies, Antinuclear / Q000276:immunology* / Q000378:metabolism*; D001172:Arthritis, Rheumatoid / Q000097:blood / Q000276:immunology; D002384:Catalysis; D003602:Cytotoxicity, Immunologic; D004278:DNA, Superhelical / Q000378:metabolism; D006801:Humans; D007739:L Cells; D008180:Lupus Erythematosus, Systemic / Q000097:blood / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D019463:Mice, Inbred MRL lpr; D012595:Scleroderma, Systemic / Q000097:blood / Q000276:immunology; D013379:Substrate Specificity
D000974:Antibodies, Antinuclear; D004278:DNA, Superhelical