Accelerated telomere shortening following allogeneic transplantation is independent of the cell source and occurs within the first year post transplant.
Telomere shortening has been documented in the blood cells of recipients of allogeneic bone marrow transplants compared with their donors. Allogeneic peripheral blood progenitor cells (PBPCs) have been increasingly used as an alternative to bone marrow. Their advantages include earlier engraftment and immune reconstitution following transplantation. We have measured telomere length of neutrophils and T cells in fully engrafted recipients of allogeneic bone marrow (n = 19) and allogeneic PBPC (n = 17) and also measured sequential telomere length in four patients after transplantation. Overall, significant telomere shortening occurred in recipients in neutrophils (0.3 kb, P < 0.001) and T cells (0.2 kb, P = 0.045). The data demonstrate that first, the degree of shortening was the same for BM and PBPC transplants and was not related to the time taken to engraft neutrophils and platelets and second, telomere shortening occurs in the first year post transplant without further shortening during the period of observation. These data suggest that the superiority of engraftment seen in PBPC transplants is independent of telomere shortening and other mechanisms such as homing or seeding may be more important.
Robertson
J D
JD
CRC Laboratory of Experimental Hematology, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester, UK.
Testa
N G
NG
Russell
N H
NH
Jackson
G
G
Parker
A N
AN
Milligan
D W
DW
Stainer
C
C
Chakrabarti
S
S
Dougal
M
M
Chopra
R
R
D000293:Adolescent; D000328:Adult; D000368:Aged; D001773:Blood Cells / Q000637:transplantation; D016026:Bone Marrow Transplantation / Q000009:adverse effects; D006085:Graft Survival; D018380:Hematopoietic Stem Cell Transplantation / Q000009:adverse effects*; D006801:Humans; D008875:Middle Aged; D009504:Neutrophils / Q000648:ultrastructure; D013601:T-Lymphocytes / Q000648:ultrastructure; D016615:Telomere / Q000378:metabolism* / Q000648:ultrastructure; D013997:Time Factors; D014184:Transplantation, Homologous / Q000009:adverse effects