title: Effects of cholesterol and temperature on the permeability of dimyristoylphosphatidylcholine bilayers near the chain melting phase transition.
abstract: The passive leakage of glucose across bilayers of dimyristoylphosphatidylcholine (DMPC), cholesterol (variable), and dicetyl phosphate (constant 5.9 mol%) has been measured as efflux over 30 min from multilamellar vesicles. Bilayer cholesterol was varied from 20 mol% to 40 mol%. Glucose permeation rates were measured from 10 degrees C to 36 degrees C, and showed a maximum in permeability at 24 degrees C, the DMPC phase transition temperature. Increasing the bilayer cholesterol content above 20 mol% reduced that permeability peak. These results are quite consistent with a large number of similar bilayer permeability studies over the past 25 years. However, they are not consistent with a previous study of these same systems, which reported increased glucose permeability with temperature, without any maximum at or near the lipid chain melting temperature (K. Inoue, Biochim. Biophys. Acta 339 (1974) 390-402).
authors.affiliation: Department of Physics and Astronomy, University of Maine, 5709 Bennett Hall, Orono, ME 04469-5709, USA.
authors.forename: W V
authors.initials: WV
authors.lastname: Kraske
authors.forename: D B
authors.initials: DB
authors.lastname: Mountcastle
mesh_terms: D002784:Cholesterol / Q000494:pharmacology*; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry*; D005947:Glucose / Q000737:chemistry; D008051:Lipid Bilayers / Q000737:chemistry*; D010539:Permeability / Q000187:drug effects; D013696:Temperature
chemical_list: D008051:Lipid Bilayers; D002784:Cholesterol; D005947:Glucose; D004134:Dimyristoylphosphatidylcholine