title: Effects of ozone and endotoxin coexposure on rat airway epithelium: potentiation of toxicant-induced alterations.
abstract: Tropospheric ozone is the major oxidizing component in photochemical smog and is one of the most pervasive problems to human health of the criteria air pollutants for which the National Ambient Air Quality Standards have been designated by the Clean Air Act. Although many adverse health effects of ozone exposure have been documented in both humans and laboratory animals, controversy surrounds the establishment and implementation of ozone standards set forth by the U.S. Environmental Protection Agency. Because people are commonly exposed to more than one air pollutant at a time, studies that examine coexposures to airborne materials may be more relevant for assessing their risks to human health. Airborne biogenic substances such as pollens, spores, and bacterial products are ubiquitous in the environment, and when inhaled can cause adverse respiratory symptoms. One such biogenic agent, bacterial endotoxin, is a potent stimulus of airway inflammation and is a ubiquitous airborne contaminant commonly found in domestic, agricultural, and industrial settings. Little is known about the interaction of exposures to biogenic substances and criteria air pollutants such as ozone. In the last few years we have performed a series of studies in rodents that examined the biologic responses of the respiratory epithelium after airway exposures to both endotoxin and ozone. When exposed to ozone (0.5 ppm 8 hr/day for 3 days), Fischer rats develop lesions in the nasal transitional epithelium, whereas intranasal instillation of endotoxin (20 microg) elicits epithelial lesions in the respiratory epithelium of the nose and conducting airways. Our studies were designed to examine how exposure to one toxicant may affect the airway epithelial lesions induced by the other toxicant. We investigated the potential role of acute inflammation in the enhancement of airway epithelial lesions after exposure of these two toxicants in neutrophil-sufficient and neutrophil-deficient rodents. A summary of these results indicates that epithelial and inflammatory responses to coexposure of these two pollutants are greater than those elicited by either agent alone. Interestingly, each toxicant enhances the epithelial alterations induced by the other. Furthermore, the synergistic effects elicited by coexposure to ozone and endotoxin are mediated partly by neutrophils. These studies provided some new insights into how inhaled co-pollutants interact to initiate and promote alterations of airway epithelium. Further studies with these and other air pollutants will help define their true risk to human health.
authors.affiliation: Department of Pathology and Diagnostic Investigation, College of Veterinary Medicine, Michigan State University, East Lansing, Michigan 48824, USA.
authors.forename: J G
authors.initials: JG
authors.lastname: Wagner
authors.forename: J A
authors.initials: JA
authors.lastname: Hotchkiss
authors.forename: J R
authors.initials: JR
authors.lastname: Harkema
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004347:Drug Interactions; D004731:Endotoxins / Q000633:toxicity*; D004848:Epithelium / Q000187:drug effects; D008168:Lung / Q000187:drug effects / Q000473:pathology*; D008297:Male; D008679:Metaplasia / Q000139:chemically induced*; D009504:Neutrophils / Q000187:drug effects; D010126:Ozone / Q000633:toxicity*; D011550:Pseudomonas aeruginosa; D051381:Rats; D011916:Rats, Inbred F344; D020545:Respiratory Mucosa / Q000187:drug effects; D012137:Respiratory System / Q000187:drug effects*
chemical_list: D004731:Endotoxins; D010126:Ozone