Characterization of the HCV core virus-like particles produced in the methylotrophic yeast Pichia pastoris.
Little is known about the mechanism of hepatitis C virion assembly. So the capacity of the entire Hepatitis C virus core protein (HCcAg) produced in Pichia pastoris to form particles either in its native soluble state or after detergent treatment of HCcAg associated to cell debris were studied. Size exclusion chromatography suggested that HCcAg assembled into high molecular weight structures. HCcAg was also specifically recognized by a serum from a chronic HCV carrier patient. This antigen migrated with buoyant density values similar to those obtained for native nucleocapsid particles from infected patients when analyzed using sucrose density gradient centrifugation. The analysis by electron microscopy of purified HCcAg showed aggregates resembling virus-like particles (VLPs) with an average diameter of 30 nm. These results indicated that the HCcAg obtained from P. pastoris assembled into VLPs resembling HCV nucleocapsid particles in a mature stage. Such HCcAg aggregates characterized here could be a valuable tool to elucidate the mechanisms of HCV nucleocapsid assembly.
Acosta-Rivero
N
N
Division of Vaccines, Physical Chemistry Department, Electron Microscopy Laboratory, Center for Genetic Engineering and Biotechnology, Ave. 31 e/158 y 190, C.P. 10600, C. Havana, Cuba. nelson.acosta@cigb.edu.cu
Aguilar
J C
JC
Musacchio
A
A
Falcón
V
V
Viña
A
A
de la Rosa
M C
MC
Morales
J
J
D016174:Hepacivirus / Q000737:chemistry*; D015151:Immunoblotting; D008970:Molecular Weight; D010843:Pichia / Q000821:virology*; D020673:Protein Renaturation; D014758:Viral Core Proteins / Q000737:chemistry* / Q000378:metabolism; D014771:Virion / Q000737:chemistry*
D014758:Viral Core Proteins; C069130:nucleocapsid protein, Hepatitis C virus