Site-directed alkylation to cytidine within duplex by the oligonucleotides containing functional nucleobases.
We have previously described that oligonucleotides (ODN) containing phenylsulfoxide derivative of 2-amino-6-vinylpurine nucleoside analog (1) are activated within duplex to form cross-link toward cytidine selectively at the target site. In this paper, we wish to report the search for more stable precursor susceptible for activation within duplex.
Nagatsugi
F
F
Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
Kawasaki
T
T
Tokuda
N
N
Maeda
M
M
Sasaki
S
S
D000478:Alkylation; D003432:Cross-Linking Reagents / Q000737:chemistry; D003562:Cytidine / Q000737:chemistry*; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000737:chemistry*; D011687:Purines / Q000737:chemistry; D013440:Sulfides / Q000737:chemistry*; D014753:Vinyl Compounds / Q000737:chemistry
C432203:2-amino-6-vinylpurine; D003432:Cross-Linking Reagents; D009841:Oligonucleotides; D011687:Purines; D013440:Sulfides; D014753:Vinyl Compounds; D003562:Cytidine