Ecdysteroids affect in vivo protein metabolism of the flight muscle of the tobacco hornworm (Manduca sexta).
Ecdysteroid growth promotion of the dorsolongitudinal flight muscle of Manduca sexta was studied by measuring in vivo protein metabolism using both "flooding-dose" and "non-carrier" techniques.  These procedures differ in that the former method includes injection of non-labelled phenylalanine (30 micromoles/insect) together with the [3H]amino acid.  Injected radioactivity plateaued in the haemolymph within 7 min.  With the flooding-dose method, haemolymph and intramuscular specific radioactivities were similar between 15 min and 2 h.  Incorporation of [3H]phenylalanine into muscle protein was linear with either method between 30 and 120 min.  Fractional rates (%/12 h) of synthesis with the flooding-dose technique were best measured after 1 h because of the initial delay in radioactivity equilibration.  Estimation of body phenylalanine turnover with the non-carrier method showed 24-53%/h which was negligible with the flooding-dose method.  Since the two methods yielded similar rates of protein synthesis, the large injection of non-labelled amino acid did not alter the rate of synthesis.  Because the flooding-dose technique requires only a single time point measurement, it is the preferred method.  The decline and eventual cessation of flight-muscle growth was mostly a consequence of declining protein synthesis though degradation increased between 76-86 h before eclosion and was relatively rapid.  This decline in muscle growth could be prevented by treating pupae with 20-hydroxyecdysone (10 micrograms/insect).  Protein accretion was promoted by a decline of up to 80% in protein breakdown, which was offset in part by a concurrent though much smaller decrease in protein synthesis.  Therefore, ecdysteroids may increase flight-muscle growth by inhibiting proteolysis.
Tischler
M E
ME
Department of Biochemistry, University of Arizona, Tucson 85724, USA.
Wu
M
M
Cook
P
P
Hodsden
S
S
D000818:Animals; D004441:Ecdysterone / Q000494:pharmacology* / Q000502:physiology; D005260:Female; D005426:Flight, Animal / Q000502:physiology; D008297:Male; D018402:Manduca / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D009124:Muscle Proteins / Q000096:biosynthesis / Q000187:drug effects* / Q000378:metabolism*; D011679:Pupa / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D013997:Time Factors
D009124:Muscle Proteins; D004441:Ecdysterone