title: Hindlimb-suspension and spaceflight both alter cGMP levels in rat choroid plexus.
abstract: Effects of actual and simulated weightlessness on choroidal guanylate cyclase activity were evaluated by assaying the production of cyclic guanosine monophosphate (cGMP), a second messenger involved in mechanisms regulating the secretion of cerebrospinal fluid (CSF) in choroid plexus. Cyclic cGMP was measured, using radio-immunoassay, in choroidal extracts of hindlimb-suspended rats (HLS rats), adapted to an anti-orthostatic restraint for 30 min., or for 3, 9 or 14 days and after a 17-day spaceflight (Life and Microgravity SpaceLab experiment; LMS). Basal cGMP levels were slightly but significantly decreased in the first 30 min. of the HLS experiment, whereas they were significantly increased in rats adapted to longer anti-orthostatic restraints. LMS flight rats demonstrated a similar increase in the choroidal cGMP baseline. After natriuretic peptide stimulation, i.e. using ANP (atrial natriuretic peptide) or BNP (brain natriuretic peptide), choroidal cGMP contents were typically increased (by 1.5-2 times; p<0.05) in control rats (LMS and HLS experiments), but not significantly elevated in suspended rats, except for those adapted to HLS for 14 days. In these animals the ANP-dependent cGMP production was significantly increased (by about 3 times; p<0.005). The ANP- or BNP-dependent responses were similarly abolished in LMS flight rats, which were dissected 4-6 hours after return to Earth's gravity. The role of corticosteroids was also investigated during the LMS experiment. Results on choroidal functions revealed a lack of significant change of cGMP levels between adrenalectomized and sham-operated rats. For the first time, it is reported that both basal and ANP- stimulated cGMP levels are dramatically changed over the first 14 days of suspension, i.e. with experiments known to simulate some effects of weightlessness. Basal choroidal cGMP levels are also increased after 17 days in space, suggesting that space adaptation also impacts choroidal guanylate cyclase activities. However, the absence of ANP-dependent cGMP increase, observed in LMS flight animals, suggests that HLS could not simulate all the spaceflight effects. Thus, these preliminary results seem to show that a natriuretic peptides-independent s stem is involved in choroidal adaptation to spaceflight.
authors.affiliation: Institut des Neurosciences, Universite Pierre et Marie Curie-Paris VI, France.
authors.forename: C
authors.initials: C
authors.lastname: Carcenac
authors.forename: S
authors.initials: S
authors.lastname: Herbute
authors.forename: C
authors.initials: C
authors.lastname: Masseguin
authors.forename: L
authors.initials: L
authors.lastname: Mani-Ponset
authors.forename: D
authors.initials: D
authors.lastname: Maurel
authors.forename: R
authors.initials: R
authors.lastname: Briggs
authors.forename: A
authors.initials: A
authors.lastname: Guell
authors.forename: J B
authors.initials: JB
authors.lastname: Gabrion
mesh_terms: D000222:Adaptation, Physiological; D000315:Adrenalectomy; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000494:pharmacology; D002555:Cerebrospinal Fluid / Q000378:metabolism; D002831:Choroid Plexus / Q000187:drug effects / Q000378:metabolism*; D006152:Cyclic GMP / Q000378:metabolism*; D006162:Guanylate Cyclase / Q000378:metabolism; D019417:Hindlimb Suspension; D008297:Male; D020097:Natriuretic Peptide, Brain / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013026:Space Flight; D014893:Weightlessness; D018474:Weightlessness Simulation
chemical_list: D020097:Natriuretic Peptide, Brain; D009320:Atrial Natriuretic Factor; D006162:Guanylate Cyclase; D006152:Cyclic GMP