Asymmetric inheritance of radial glial fibers by cortical neurons.
Recent studies demonstrated the neuronogenic role of radial glial cells (RGCs) in the rodent. To reveal the fate of radial glial processes, we intensively monitored divisions of RGCs in DiI-labeled slices from the embryonic day 14 mouse cortex. During RGC division, each pia-connected fiber becomes thin but is neither lost nor divided; it is inherited asymmetrically by one daughter cell. In divisions that produce a neuron and a progenitor, the neuron inherits the pial fiber, also grows a thick ventricular process for several hours, and is therefore indistinguishable from the progenitor RGC. The ventricular process in the radial glial-like neuron ("radial neuron") then collapses, leading to ascent of the neuron by using the "recycled" radial fiber.
Miyata
T
T
Laboratory for Cell Culture Development, Brain Science Institute, RIKEN, 351-0198, Saitama, Japan. tmiyata@brain.riken.go.jp
Kawaguchi
A
A
Okano
H
H
Ogawa
M
M
D000375:Aging / Q000502:physiology; D000818:Animals; D019521:Body Patterning / Q000502:physiology; D002451:Cell Compartmentation / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology*; D002455:Cell Division / Q000502:physiology*; D019070:Cell Lineage / Q000502:physiology; D002465:Cell Movement / Q000502:physiology*; D048429:Cell Size / Q000502:physiology; D002540:Cerebral Cortex / Q000166:cytology / Q000196:embryology* / Q000378:metabolism; D005333:Fetus; D007150:Immunohistochemistry; D051379:Mice; D008813:Mice, Inbred ICR; D009419:Nerve Tissue Proteins / Q000378:metabolism; D016501:Neurites / Q000378:metabolism / Q000648:ultrastructure; D009457:Neuroglia / Q000166:cytology* / Q000378:metabolism; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D009924:Organ Culture Techniques; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism
D009419:Nerve Tissue Proteins