Corneal endothelial response to induced myopia in the chicken.
OBJECTIVE
To investigate the role of corneal endothelial surface enlargement in the chicken myopia model in inducing corneal endothelial changes.


METHODS
Lid suture was performed on one eye of 1-day-old cockerels. Five chickens were killed at 1 week, and four chickens killed at each of 3 weeks, 6 weeks, and 10 weeks postnatal. The endothelial morphology was obtained by flat mounting the endothelial surface and the subsequent digitisation. Comparisons were undertaken between the control unsutured eye and the lid-sutured eye endothelium, and between the central endothelial areas compared to the peripheral endothelial areas in both the myopic and the normal corneas. Calculation of the contribution to the endothelial change by hypertrophy and mitosis were calculated using Bahn's formula.


RESULTS
Total endothelial surface area increased significantly over time in the myopic model compared to control eyes but the mean cell area of endothelial cells remained the same for both the enlarged myopic endothelial surface area and in the normal controls. Sampling from the central and the peripheral corneal endothelial surface also disclosed no difference. The mean cell area did increase steadily with age but was the same for both normal and myopic corneas.


CONCLUSIONS
It would appear that there are equal contributions from hypertrophy and mitosis in the myopic group and the normal corneal group with a slightly increasing trend towards mitotic activity in the myopic corneal endothelial layer.
Hirst
L W
LW
Department of Surgery, University of Queensland, St Lucia, Australia. lawrie.hirst@pbf.org.au
Bancroft
J
J
Bi
J Q
JQ
Ohirich
S
S
D000818:Animals; D002645:Chickens; D004728:Endothelium, Corneal / Q000473:pathology*; D006984:Hypertrophy; D008938:Mitosis; D009216:Myopia / Q000209:etiology / Q000473:pathology*; D012683:Sensory Deprivation