Parasite persistence in treated chagasic patients revealed by xenodiagnosis and polymerase chain reaction.
Polymerase chain reaction (PCR) was compared with xenodiagnosis performed 20 years after trypanocidal chemotherapy to investigate parasite clearance. Eighty-five seropositive individuals for Chagas disease presenting a positive xenodiagnosis were treated with specific drugs; 37 in the acute phase and 48 in the chronic phase. Fifteen chronic asymptomatic patients received a placebo. Treatment in the acute phase led to PCR negative results in 73% of the cases, while xenodiagnosis was negative in 86%. In the chronic phase, PCR was negative in 65% of the patients and 83% led to xenodiagnosis negative results. Regarding the untreated group (placebo), 73% gave negative results by xenodiagnosis, of which 36% were positive by PCR. Individuals that were considered seronegative (n=10), presented unequivocally negative results in the PCR demonstrating the elimination of parasite DNA. Seventeen individuals had their antibodies titers decreased to such a level that the final results were considered as doubtful and 16 of them presented negative PCR. The molecular method represents a clear advantage over conventional techniques to demonstrate persistent infections in Chagas disease patients that underwent chemotherapy.
Britto
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Departamento de Bioquímica e Biologia Molecular, Instituto Oswaldo Cruz-Fiocruz, 21045-900 Rio de Janeiro, RJ, Brasil. cbritto@gene.dbbm.fiocruz.br
Silveira
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Cardoso
M A
MA
Marques
P
P
Luquetti
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A
Macêdo
V
V
Fernandes
O
O
D000208:Acute Disease; D000818:Animals; D014355:Chagas Disease / Q000188:drug therapy / Q000469:parasitology*; D016009:Chi-Square Distribution; D002908:Chronic Disease; D018105:DNA, Kinetoplast / Q000032:analysis; D005500:Follow-Up Studies; D006801:Humans; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D014344:Trypanocidal Agents / Q000627:therapeutic use; D020525:Xenodiagnosis
D018105:DNA, Kinetoplast; D014344:Trypanocidal Agents