title: Induction of direct somatic embryogenesis in garlic (Allium sativum).
abstract: An efficient novel method of direct somatic embryogenesis from basal tissue of garlic clove was developed. The influence of plant growth regulators, basal medium and explant type on somatic embryo induction was examined. The best plant growth regulator combination was, 2,4-D and kinetin at 1.0 mg/L and 0.5 mg/L respectively, inducing direct somatic embryogenesis in 60% of explants. White's medium was used as basal medium and somatic embryos developed on explants after six weeks. The technique has potential applicability for problems associated with plant regeneration and virus elimination in garlic.
authors.affiliation: Department of Biosciences, Saurashtra University, Rajkot - 360 005, India.
authors.forename: S J
authors.initials: SJ
authors.lastname: Sata
authors.forename: S B
authors.initials: SB
authors.lastname: Bagatharia
authors.forename: V S
authors.initials: VS
authors.lastname: Thaker
mesh_terms: D015084:2,4-Dichlorophenoxyacetic Acid / Q000494:pharmacology; D000225:Adenine / Q000031:analogs & derivatives* / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D005737:Garlic / Q000166:cytology / Q000196:embryology*; D007701:Kinetin; D010937:Plant Growth Regulators / Q000494:pharmacology; D018547:Plant Stems / Q000166:cytology* / Q000196:embryology; D012038:Regeneration / Q000187:drug effects / Q000502:physiology*
chemical_list: D010937:Plant Growth Regulators; D015084:2,4-Dichlorophenoxyacetic Acid; D000225:Adenine; D007701:Kinetin