title: Methylobacillus flagellatus KT contains a novel cbo-type cytochrome oxidase.
abstract: The o-type oxidase from the methanol-grown obligate methylotroph Methylobacillus flagellatus KT has been purified to homogeneity. The complex is composed of four subunits (57, 40, 35 and 30 kDa). It contains six haems (4C:1B:1O) and one copper atom per molecule. It is proposed that the haem O-Cu(B) binuclear centre and a low-spin haem B are located in subunit I (57 kDa), two haems C reside in the cytochrome c homodimer (35 kDa), two haems C belong to the dihaem cytochrome c (30 kDa). The presented data provide evidence that cytochrome cbo is a novel representative of the haem-copper oxidase superfamily.
authors.affiliation: Department of Microbiology, Moscow State University, Lenin's Hills, Moscow 119899, Russia.
authors.forename: E V
authors.initials: EV
authors.lastname: Strom
authors.forename: T Y
authors.initials: TY
authors.lastname: Dinarieva
authors.forename: A I
authors.initials: AI
authors.lastname: Netrusov
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003573:Cytochrome b Group / Q000737:chemistry / Q000378:metabolism; D003574:Cytochrome c Group / Q000737:chemistry / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D006418:Heme / Q000737:chemistry; D020631:Methylobacillus / Q000201:enzymology*
chemical_list: D003573:Cytochrome b Group; D003574:Cytochrome c Group; D006418:Heme; C038862:cytochrome o oxidase; D003576:Electron Transport Complex IV