A novel approach for the solid phase synthesis of DNA-peptide conjugates.
The strategy of this study involves automated synthesis of oligonucleotides on a CPG support using standard cyanoethyl phosphoramidite chemistry followed by covalent linkage to peptide fragments bearing a free terminal alpha-amino group and residues with protected side chains. Conjugation was formed through an alkyldiisocyanate linker. Conjugates were isolated by cleavage from the solid support and deprotection in one step.
Kubo
T
T
Department of Chemistry, Kyushu School of Engineering, Kinki University, 11-6 Kayanomori, Iizuka, Fukuoka 820-8555, Japan.
Dubey
K
K
Fujii
M
M
D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000138:chemical synthesis* / Q000737:chemistry; D009841:Oligonucleotides / Q000138:chemical synthesis*; D010446:Peptide Fragments / Q000138:chemical synthesis* / Q000737:chemistry
D009841:Oligonucleotides; D010446:Peptide Fragments; D004247:DNA