title: Single ion actions: the induction of micronuclei in V79 cells exposed to individual protons.
abstract: Understanding the effects of single-particles from conventional radiation biology experiments is problematic due to the stochastics of particle tracks. This complicates the determinations of risk associated with low doses. We have developed a charged particle microbeam, which allows individually counted particles to be delivered to precise cellular locations. The system is capable of delivering a single charged particle with > 99% efficiency. Of these particles 90% are delivered with a resolution of +/- 2 micrometers and 96% with a resolution of +/- 5 micrometers. We have carried out preliminary studies in Chinese hamster V79 cells to monitor the effectiveness of low energy protons at inducing cytological damage. We have used the micronucleus assay as a measure of predominantly lethal chromosome damage. The effects of a single 3.2 MeV proton delivered individually to cells could be measured, with less than 2% of the exposed cells producing micronuclei 24 hours later. The yield of micronuclei formation was essentially linear up to the highest dose (30 particles per cell nucleus) delivered. Ultimately, the ability to target particles to different parts of the cell nucleus may start to impact on models available for chromosome aberration formation and chromosomal Organisation and mechanisms underlying genomic instability.
authors.affiliation: Gray Laboratory Cancer Research Trust, Mount Vernon Hospital, Northwood, UK.
authors.forename: K M
authors.initials: KM
authors.lastname: Prise
authors.forename: M
authors.initials: M
authors.lastname: Folkard
authors.forename: A M
authors.initials: AM
authors.lastname: Malcolmson
authors.forename: C H
authors.initials: CH
authors.lastname: Pullar
authors.forename: G
authors.initials: G
authors.lastname: Schettino
authors.forename: A G
authors.initials: AG
authors.lastname: Bowey
authors.forename: B D
authors.initials: BD
authors.lastname: Michael
mesh_terms: D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured / Q000187:drug effects / Q000528:radiation effects*; D002875:Chromosomes / Q000528:radiation effects; D006224:Cricetinae; D003412:Cricetulus; D003571:Cytochalasin B / Q000494:pharmacology; D004307:Dose-Response Relationship, Radiation; D018499:Linear Energy Transfer; D048629:Micronuclei, Chromosome-Defective / Q000528:radiation effects*; D010315:Particle Accelerators / Q000295:instrumentation; D011522:Protons; D011829:Radiation Dosage; D011853:Radiobiology / Q000295:instrumentation
chemical_list: D011522:Protons; D003571:Cytochalasin B