Dual Y-chromosome painting and immunofluorescence staining of archival human liver transplant biopsies.
Combining fluorescence in situ hybridization (FISH) and indirect immunofluorescence staining of protein markers provides a highly specific method for identifying chromosomes in phenotypically defined cells and tissues. We developed a technique enabling dual chromosome painting and immunofluorescence staining of archival formalin-fixed, paraffin-embedded material, and used this to phenotype chimeric cells in female-to-male human liver transplants.
Dundas
S R
SR
Department of Clinical and Surgical Sciences, University of Edinburgh, Scotland, UK. srdundas@yahoo.co.uk
Boyle
S
S
Bellamy
C O
CO
Hawkins
W
W
Garden
O J
OJ
Ross
J A
JA
Bickmore
W
W
D020223:Chromosome Painting; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D008099:Liver / Q000648:ultrastructure*; D016031:Liver Transplantation; D008297:Male; D010641:Phenotype; D014998:Y Chromosome