Synthesis of oligonucleoside boranophosphates via an H-phosphonate method without nucleobase protection.
Short oligonucleoside boranophosphates containing all four nucleosides were synthesized on solid support using base-unprotected nucleoside H-phosphonate monomers. This strategy avoided irreversible base modifications during the boronation procedure. Structures of the boranophosphate oligomers were confirmed by 1H, 31P, 10B NMR and MS analysis as well as by enzymatic hydrolysis.
Sergueev
D S
DS
Department of Chemistry, Duke University, Durham, North Carolina 27708-0346, USA.
Sergueeva
Z A
ZA
Shaw
B R
BR
D001896:Boron Compounds / Q000138:chemical synthesis*; D002851:Chromatography, High Pressure Liquid; D009682:Magnetic Resonance Spectroscopy; D009841:Oligonucleotides / Q000138:chemical synthesis*; D063065:Organophosphonates / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D001896:Boron Compounds; D009841:Oligonucleotides; D063065:Organophosphonates