title: Muscle atrophy associated with microgravity in rat: basic data for countermeasures.
abstract: Morphological, contractile properties and myosin heavy chain (MHC) composition of rat soleus muscles were studied after 2 weeks of unloading (HS) and after 2 weeks of HS associated with selective deafferentation (HS + DEAF) at the level L4 and L5. The same significant reductions in muscle mass and tetanic tension were found after HS and HS + DEAF. However, the transformation of the slow-twitch soleus muscle towards a faster type characterized by a decrease in twitch time parameters and an increase in fast-twitch type MHC isoforms in HS did not appear in HS + DEAF conditions. Our results also showed that a pattern similar to firing rate of motoneurones innervating slow-twitch muscles inhibited the slow to fast fiber changes observed during HS. Nevertheless, neither the loss of mass or force output in the HS muscles were prevented by electrostimulation. Immobilization in a stretched position during HS maintained the muscle wet weight, mechanical and electrophoretical characteristics close to control values. We concluded that the decrease in mechanical strains imposed on the muscle during unloading was the main factor for the development of atrophy, while the kinetic changes might be predominantly modulated by the nervous command. These basic data suggested that some experimental conditions such as electrostimulation or stretching, could participate in countermeasure programmes.
authors.affiliation: Laboratoire de Physiologie des Structures Contractiles, Universite des Sciences et Technologies de Lille, France.
authors.forename: M
authors.initials: M
authors.lastname: Falempin
authors.forename: Y
authors.initials: Y
authors.lastname: Mounier
mesh_terms: D000344:Afferent Pathways / Q000601:surgery; D000818:Animals; D004599:Electric Stimulation Therapy; D019417:Hindlimb Suspension; D008297:Male; D009119:Muscle Contraction; D018482:Muscle, Skeletal / Q000378:metabolism / Q000502:physiology*; D009133:Muscular Atrophy / Q000209:etiology / Q000517:prevention & control*; D018995:Myosin Heavy Chains / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013314:Stress, Mechanical; D014893:Weightlessness / Q000009:adverse effects; D018544:Weightlessness Countermeasures; D018474:Weightlessness Simulation
chemical_list: D018995:Myosin Heavy Chains