Recognition of DNA by strand invasion with oligonucleotide-spermine conjugates.
Modified oligonucleotides bearing spermine groups (ODN-sper) with increased binding affinity to DNA have been synthesized. The ability of these ODN-sper to bind within superhelical double-stranded DNA by strand invasion has been studied. The uptake by a supercoiled plasmid was 3 fold higher for the ODN-sper than for the unmodified oligonucleotides.
Potier
P F
PF
Laboratoire de Chimie Génétique, UMR 7514, Faculté de Pharmacie de Strasbourg, BP 24, F-67401 Illkirch, France.
Behr
J P
JP
D004278:DNA, Superhelical / Q000737:chemistry*; D008156:Luciferases / Q000235:genetics; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000737:chemistry* / Q000235:genetics; D010957:Plasmids / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013096:Spermine / Q000031:analogs & derivatives* / Q000737:chemistry
D004278:DNA, Superhelical; D009841:Oligonucleotides; D013096:Spermine; D008156:Luciferases