Selection of genomic target RNAs by iterative screening.
A growing number of proteins are known to exert their regulatory or biological functions via RNA binding. In some cases genetic interactions allow us to infer candidate targets for RNA directed regulation, but in many other cases identification of potential regulatory targets is problematic. We have developed an in vitro biochemical screen, SETIS (SElection of <<genomic>> Target RNAs by Iterative Screening) that allows screening of a major portion of the genome for identification of potential targets for RNA binding proteins.
Brunel
C
C
UPR 9002 du CNRS, Institut de Biologie Cellulaire et Moléculaire, 15 rue Descartes, 67084 Cedex, Strasbourg, France. c.brunel@ibmc.u-strasbg.fr
Ehresmann
B
B
Ehresmann
C
C
McKeown
M
M
D000818:Animals; D017123:Bacteriophage T7 / Q000201:enzymology; D016367:Baculoviridae / Q000201:enzymology; D001483:Base Sequence; D001665:Binding Sites; D004262:DNA Restriction Enzymes / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D029721:Drosophila Proteins; D004331:Drosophila melanogaster; D005260:Female; D015698:Genomic Library; D008962:Models, Theoretical; D008969:Molecular Sequence Data; D010957:Plasmids; D016133:Polymerase Chain Reaction; D011506:Proteins / Q000378:metabolism; D012313:RNA / Q000032:analysis / Q000096:biosynthesis / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000737:chemistry*; D012261:Ribonucleoproteins / Q000378:metabolism*; D017423:Sequence Analysis, RNA / Q000379:methods; D017385:Sequence Homology
D029721:Drosophila Proteins; D011506:Proteins; D016601:RNA-Binding Proteins; D012261:Ribonucleoproteins; C066174:tra2 protein, Drosophila; D012313:RNA; D012321:DNA-Directed RNA Polymerases; D004262:DNA Restriction Enzymes