title: Effect of calcium hydroxide form and placement on root dentine pH.
abstract: OBJECTIVE
  The aim of this study was to measure variations in dentinal pH following the placement of various forms of calcium hydroxide in either the root canal or the pulp chamber.
  
  
  METHODS
  Extracted single-rooted human teeth were prepared, and the root canals instrumented using a conventional technique. Three cavities were drilled through the root dentine to within 1 mm of the canal wall at the cervical, middle and apical thirds. A total of 125 teeth were randomly divided into five groups; group 1: pure aqueous calcium hydroxide paste (calcium hydroxide/distilled water solution) was placed in the root canal; group 2: the same aqueous calcium hydroxide paste was placed in the pulp chamber; group 3: Hycal, a new form of calcium hydroxide paste, was placed in the pulp chamber; group 4: calcium hydroxide gutta-percha points were placed in the root canal; group 5: control group, wet canal (distilled water) without medication. The access cavities and apical ends were sealed, and the teeth were placed in individual vials containing phosphate-buffered saline, and stored at 37 degrees C. The pH was measured in the dentinal cavities at 8 h and at 1, 2, 3, 7, 14, and 21 days using a calibrated microelectrode.
  
  
  RESULTS
  At 8 h and 1, 2, and 3 days, the highest pH values were obtained when the aqueous calcium hydroxide paste was placed in the pulp chamber. At 7 days, the pH had increased in the Hycal group without being significantly different from the aqueous calcium hydroxide paste placed either in the root canal or in the pulp chamber. At 14 days, Hycal( had the highest pH values (pH 10.65); however, at 21 days no significant difference was noted amongst these first three groups. Control group values ranged from 7.88 to 8.60; the pH created by the calcium hydroxide gutta-percha points was lower than for the control group. Whatever the product or placement location, cervical pH was similar to middle pH, and greater than apical. However, there was no significant difference between the three when all groups were combined. Overall, aqueous calcium hydroxide paste placed in the pulp chamber provided the highest pH values during the experiment, except at day 14. The aqueous calcium hydroxide paste placed in the root canal or Hycal had similar values at days 7 and 21.
  
  
  CONCLUSIONS
  Under the conditions of this study, an aqueous calcium hydroxide paste placed in the pulp chamber increased dentinal pH more than the other techniques. The pH of dentine is affected by the form of calcium hydroxide used.
authors.affiliation: Department of Conservative Dentistry-Endodontics, Faculty of Dentistry, University Victor Segalen, Bordeaux, France. Fabienne.Perez@odonto.u-bordeaux2.fr
authors.forename: F
authors.initials: F
authors.lastname: Pérez
authors.forename: M
authors.initials: M
authors.lastname: Franchi
authors.forename: J F
authors.initials: JF
authors.lastname: Péli
mesh_terms: D002126:Calcium Hydroxide / Q000008:administration & dosage*; D003786:Dental Pulp Cavity; D003804:Dentin / Q000737:chemistry / Q000187:drug effects*; D006801:Humans; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D006878:Hydroxides; D012388:Root Canal Irrigants / Q000008:administration & dosage*; D014092:Tooth Root / Q000187:drug effects*
chemical_list: D006878:Hydroxides; D012388:Root Canal Irrigants; C031356:hydroxide ion; D002126:Calcium Hydroxide