title: Mapping B-cell epitopes of hepatitis C virus E2 glycoprotein using human monoclonal antibodies from phage display libraries.
abstract: Clinical and experimental evidence indicates that the hepatitis C virus (HCV) E2 glycoprotein (HCV/E2) is the most promising candidate for the development of an effective anti-HCV vaccine. Identification of the human epitopes that are conserved among isolates and are able to elicit protective antibodies would constitute a significant step forward. This work describes the mapping of the B-cell epitopes present on the surface of HCV/E2, as recognized by the immune system during infection, by the analysis of the reciprocal interactions of a panel of human recombinant Fabs derived from an HCV-infected patient. Three unrelated epitopes recognized by antibodies with no neutralization-of-binding (NOB) activity were identified; a fourth, major epitope was defined as a clustering of minor epitopes recognized by Fabs endowed with strong NOB activity.
authors.affiliation: Istituti di Microbiologia, Facoltà di Medicina e Chirurgia, Università Cattolica del S. Cuore, 00168 Rome, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Bugli
authors.forename: N
authors.initials: N
authors.lastname: Mancini
authors.forename: C Y
authors.initials: CY
authors.lastname: Kang
authors.forename: C
authors.initials: C
authors.lastname: Di Campli
authors.forename: A
authors.initials: A
authors.lastname: Grieco
authors.forename: A
authors.initials: A
authors.lastname: Manzin
authors.forename: A
authors.initials: A
authors.lastname: Gabrielli
authors.forename: A
authors.initials: A
authors.lastname: Gasbarrini
authors.forename: G
authors.initials: G
authors.lastname: Fadda
authors.forename: P E
authors.initials: PE
authors.lastname: Varaldo
authors.forename: M
authors.initials: M
authors.lastname: Clementi
authors.forename: R
authors.initials: R
authors.lastname: Burioni
mesh_terms: D000911:Antibodies, Monoclonal / Q000235:genetics / Q000276:immunology*; D001402:B-Lymphocytes / Q000821:virology*; D018604:Epitope Mapping; D016174:Hepacivirus / Q000276:immunology / Q000378:metabolism*; D006526:Hepatitis C / Q000276:immunology* / Q000517:prevention & control; D018936:Hepatitis C Antigens / Q000737:chemistry / Q000276:immunology*; D006801:Humans; D019151:Peptide Library; D014759:Viral Envelope Proteins / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D018936:Hepatitis C Antigens; D019151:Peptide Library; D014759:Viral Envelope Proteins; C091015:glycoprotein E2, Hepatitis C virus