Human T-cell leukemia virus type-I (HTLV-I) tax is not the only one factor to enhance human immunodeficiency virus type-I (HIV-1) infection in culture-supernatants.
It is hypothesized that supernatants from cell cultures contain several factors to modify the human immunodeficiency virus type-1 (HIV-1) infection. Single round infection with pseudotyped viruses with envelope from HIV-1, amphotropic murine leukemia virus (A-MLV) and vesicular stomatitis virus G-protein (VSV-G) carrying luciferase reporter gene detected that not only human T-cell leukemia/lymphoma virus type-I (HTLV-1) transformed cells but also HTLV-I-unrelated T-cells and BJA-B cells released factors enhancing the infection with all pseudotyped viruses in their culture-supernatants. No supernatants upregulated the level of transcription from transfected DNA probe. suggesting that the action of supernatants is different from that of tumor necrosis factor (TNF) and Tax of HTLV-I. These results indicated that factors not always related to HTLV-I were ubiquitously produced and promoted viral infections, probably due to non-specific enhancement of early phase of the infection.
Tempaku
A
A
Department of Biodefence and Medical Virology, Kumamoto University School of Medicine, Japan.
Maeda
Y
Y
Song
W
W
Harada
S
S
D001402:B-Lymphocytes / Q000378:metabolism*; D015704:CD4 Antigens / Q000096:biosynthesis; D002461:Cell Line, Transformed; D017077:Culture Media, Conditioned; D016356:Gene Products, tax / Q000235:genetics / Q000378:metabolism*; D015497:HIV-1 / Q000472:pathogenicity*; D015368:Human T-lymphotropic virus 1 / Q000378:metabolism*; D006801:Humans; D019169:Jurkat Cells; D020014:K562 Cells; D008562:Membrane Glycoproteins; D019713:Receptors, CCR5 / Q000096:biosynthesis; D019718:Receptors, CXCR4 / Q000096:biosynthesis; D013601:T-Lymphocytes / Q000378:metabolism*; D014759:Viral Envelope Proteins / Q000378:metabolism
D015704:CD4 Antigens; D017077:Culture Media, Conditioned; C028391:G protein, vesicular stomatitis virus; D016356:Gene Products, tax; D008562:Membrane Glycoproteins; D019713:Receptors, CCR5; D019718:Receptors, CXCR4; D014759:Viral Envelope Proteins