RNA and N3'-->P5' kissing aptamers targeted to the trans-activation responsive (TAR) RNA of the human immunodeficiency virus-1.
We used in vitro selection to identify RNA aptamers able to selectively bind to the TAR RNA motif of HIV-1, an unperfect RNA hairpin involved in the transcription of the retroviral genome. We selected aptameric RNA hairpins giving rise to kissing complexes with TAR. The N3'-->P5' phosphoramidate variant of the aptamer bind to TAR with a Kd in the low nanomolar range. However, only the RNA-RNA loop-loop complex is recognized by the Rop protein of E. coli which is specific for kissing complexes.
Darfeuille
F
F
INSERM U386, Université Victor Segalen, Bordeaux, France.
Cazenave
C
C
Gryaznov
S
S
Ducongé
F
F
Di Primo
C
C
Toulmé
J J
JJ
D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D016325:HIV Long Terminal Repeat / Q000235:genetics*; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000235:genetics / Q000494:pharmacology; D012313:RNA / Q000235:genetics* / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D020218:Response Elements / Q000235:genetics; D013379:Substrate Specificity; D020349:Surface Plasmon Resonance; D015533:Transcriptional Activation
D001426:Bacterial Proteins; D009841:Oligonucleotides; D016601:RNA-Binding Proteins; C054243:Rop protein, ColE1 plasmid; D012313:RNA