title: Specific cytotoxicity of antibody to YAL-198, a sperm antigen peptide, to murine zygote.
abstract: Active immunization with the peptide segments rSMP-230 and YAL-198, corresponding to the hydrophilic extracellular domain of two human sperm antigens (rSMP-B and YWK-II, respectively), reduced fertility in female rats by different mechanisms. The anti-rSMP-230 antibody interferes with human and murine fertilization, and the anti-YAL-198 antibody blocks the development of mouse embryos. The authors examined in vitro at which stage the antibodies to rSMP-230 and YAL-198 were cytotoxic to murine embryos up to morula/blastocyst stage. Anti-rSMP-230 antibody was not cytotoxic to any stages. On the other hand, the anti-YAL-198 antibody arrested the growth of embryos at the 2-cell stage but not at more advanced developmental stages. When the anti-YAL-198 antibody was used, spotty staining was observed only on the surfaces of embryos that had arrested at the 2-cell stage. Unstained embryos, however, continued to develop normally. In contrast, the anti-rSMP-230 antibody stained murine sperm but failed to stain murine ova and embryos. The present results suggest that the human sperm components rSMP-B and YWK-II play important roles in sperm-egg interaction and early development of the embryo, respectively.
authors.affiliation: Department of Obstetrics and Gynecology, School of Medicine, The University of Tokushima, Japan. kamada@clin.med.tokushima-u.ac.jp
authors.forename: M
authors.initials: M
authors.lastname: Kamada
authors.forename: M
authors.initials: M
authors.lastname: Takikawa
authors.forename: M
authors.initials: M
authors.lastname: Maegawa
authors.forename: S
authors.initials: S
authors.lastname: Yamamoto
authors.forename: S
authors.initials: S
authors.lastname: Yamano
authors.forename: M
authors.initials: M
authors.lastname: Irahara
authors.forename: T
authors.initials: T
authors.lastname: Aono
authors.forename: S
authors.initials: S
authors.lastname: Futaki
authors.forename: Y
authors.initials: Y
authors.lastname: Ohmoto
authors.forename: S
authors.initials: S
authors.lastname: Koide
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000941:Antigens / Q000276:immunology*; D003602:Cytotoxicity, Immunologic; D005314:Embryonic and Fetal Development / Q000276:immunology; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D008297:Male; D051381:Rats; D013084:Sperm-Ovum Interactions / Q000276:immunology; D013094:Spermatozoa / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000941:Antigens