title: Dual Y-chromosome painting and immunofluorescence staining of archival human liver transplant biopsies.
abstract: Combining fluorescence in situ hybridization (FISH) and indirect immunofluorescence staining of protein markers provides a highly specific method for identifying chromosomes in phenotypically defined cells and tissues. We developed a technique enabling dual chromosome painting and immunofluorescence staining of archival formalin-fixed, paraffin-embedded material, and used this to phenotype chimeric cells in female-to-male human liver transplants.
authors.affiliation: Department of Clinical and Surgical Sciences, University of Edinburgh, Scotland, UK. srdundas@yahoo.co.uk
authors.forename: S R
authors.initials: SR
authors.lastname: Dundas
authors.forename: S
authors.initials: S
authors.lastname: Boyle
authors.forename: C O
authors.initials: CO
authors.lastname: Bellamy
authors.forename: W
authors.initials: W
authors.lastname: Hawkins
authors.forename: O J
authors.initials: OJ
authors.lastname: Garden
authors.forename: J A
authors.initials: JA
authors.lastname: Ross
authors.forename: W
authors.initials: W
authors.lastname: Bickmore
mesh_terms: D020223:Chromosome Painting; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D008099:Liver / Q000648:ultrastructure*; D016031:Liver Transplantation; D008297:Male; D010641:Phenotype; D014998:Y Chromosome