Archaebacterial class I and class II aldolases from extreme halophiles.
Both, class I (Schiff-base forming) and class II (metal requiring) fructose biphosphate aldolases were found to be distributed among halophilic archaebacteria. The aldolase activity from Halobacteriium halobium, H. salinarium, H. cutirubrum, H. mediterranei  and H. volcanii exhibited properties of a bacterial class II aldolase as it was metal-dependent for activity and therefore inhibited by EDTA. In contrast, aldolase from H. saccharovorum, Halobacterium R-113, H. vallismortis and Halobacterium CH-1 formed a Schiff-base intermediate with the substrate and therefore resembled to eukaryotic class I type. The type of aldolase did not vary by changes in the growth medium.
Altekar
W
W
Biochemistry Division, Bhabha Atomic Research Centre, Bombay, India.
Dhar
N M
NM
D004099:Dihydroxyacetone Phosphate; D004492:Edetic Acid / Q000378:metabolism; D005634:Fructose-Bisphosphate Aldolase / Q000145:classification* / Q000378:metabolism*; D006217:Halobacterium / Q000145:classification / Q000201:enzymology*; D019617:Halobacterium salinarum; D010802:Phylogeny
D004099:Dihydroxyacetone Phosphate; D004492:Edetic Acid; D005634:Fructose-Bisphosphate Aldolase