Effect of steroid-liposome on immunohistopathology of IgA nephropathy in ddY mice.
Immunopathological studies were performed to determine whether glomerular injuries in ddY mice, a spontaneous animal model for IgA nephropathy, are influenced by treatment with a newly developed liposome loaded with prednisolone phosphate (PSL-liposome). The newly synthesized novel cationic lipid 3,6-dipentadeciroxy-1-amizino-benzene (TRX-20) was employed to obtain selective affinity to the anionic cell surface and extracellular matrices in glomerular mesangial lesions. ddY mice were treated intravenously with 1.0 mg/kg of PSL-liposome once a week for 16 weeks (from 45 weeks to 61 weeks of age). ddY mice were also intravenously treated with 1.0 mg/kg of ordinary PSL once a week for the same duration. On immunofluorescence, depositions of IgA and C3 in the glomerular mesangial areas and capillary walls of PSL-liposome-treated ddY mice were markedly decreased as compared with those of ordinary PSL-treated and untreated control ddY mice. The mean intensity of IgA and C3 in glomeruli of PSL-liposome-treated ddY mice was decreased as compared with that in ordinary PSL-treated and untreated control ddY mice. Glomerular mesangial expansion in PSL-liposome-treated ddY mice was milder than that found in ordinary PSL-treated ddY mice or untreated control ddY mice. It appears that treatment with PSL-liposome is effective in improving glomerular IgA and C3 depositions and glomerular expansion in IgA nephropathy of ddY mice.
Liao
J
J
Division of Nephrology, Department of Medicine, Juntendo University School of Medicine, Tokyo, Japan.
Hayashi
K
K
Horikoshi
S
S
Ushijima
H
H
Kimura
J
J
Tomino
Y
Y
D000818:Animals; D016503:Drug Delivery Systems; D005260:Female; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes; D005922:Glomerulonephritis, IGA / Q000188:drug therapy* / Q000473:pathology*; D007678:Kidney Glomerulus / Q000473:pathology / Q000648:ultrastructure; D008081:Liposomes / Q000493:pharmacokinetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D008854:Microscopy, Electron; D011239:Prednisolone / Q000031:analogs & derivatives* / Q000493:pharmacokinetics*; D011507:Proteinuria / Q000188:drug therapy / Q000473:pathology; D012235:Rhodamines
D005456:Fluorescent Dyes; D008081:Liposomes; D012235:Rhodamines; C009022:prednisolone phosphate; D011239:Prednisolone