title: Synthesis of modified nucleoside 5'-triphosphates for in vitro selection of catalytic nucleic acids.
abstract: 2'-Modified pyrimidine nucleoside 5'-triphosphates comprising amino, imidazole and carboxylate functionality attached to the 5-position of the base were synthesized. Two different phosphorylation methods were used to optimize the yields of these highly modified triphosphates.
authors.affiliation: Department of Chemistry & Biochemistry, Ribozyme Pharmaceuticals, Inc., 2950 Wilderness Place, Boulder, Colorado 80301, USA.
authors.forename: J
authors.initials: J
authors.lastname: Matulic-Adamic
authors.forename: A T
authors.initials: AT
authors.lastname: Daniher
authors.forename: A
authors.initials: A
authors.lastname: Karpeisky
authors.forename: P
authors.initials: P
authors.lastname: Haeberli
authors.forename: D
authors.initials: D
authors.lastname: Sweedler
authors.forename: L
authors.initials: L
authors.lastname: Beigelman
mesh_terms: D004355:Drug Stability; D011742:Pyrimidine Nucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D016337:RNA, Catalytic / Q000737:chemistry*
chemical_list: D011742:Pyrimidine Nucleotides; D016337:RNA, Catalytic