Synthesis of a new 5'-O-triphosphate analog of 5-methyl 2'-O-deoxycytidine. Preliminary in vitro labelling for non-radioactive detection of DNA.
We report the synthesis of the triphosphate of 5-methyl 4-N-[6-(p-bromobenzamido)hex-1-yl]-2'-O-deoxycytidine 3A. We also analyzed the formation of intramolecular H-bonds of 5-methyl 4-N-[n-[6-(p-bromobenzamido) caproyl amino]alk-1-yl]-2'-deoxycytidine compounds, and confirmed their presence by 1H-NMR studies. In vitro DNA labeling with modified nucleotides is preliminarily evaluated.
Rodríguez-Tanty
C
C
Organic Chemistry Department, National Center for Scientific Research, Havana, Cuba.
Pérez
R
R
Miranda
J
J
Vélez-Castro
H
H
Rosado
A
A
Macías
A
A
Vidal-Alles
V
V
Araña
M J
MJ
Pérez Souto
N
N
Higginson-Clarke
D
D
Riverón
A M
AM
D004247:DNA / Q000032:analysis* / Q000378:metabolism*; D003841:Deoxycytidine / Q000031:analogs & derivatives* / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism*; D004926:Escherichia coli; D006860:Hydrogen Bonding; D015151:Immunoblotting; D009682:Magnetic Resonance Spectroscopy; D010957:Plasmids / Q000378:metabolism; D016339:Spectrometry, Mass, Fast Atom Bombardment
D003841:Deoxycytidine; D004247:DNA; C016569:5-methyldeoxycytidine