Mature phenotype in Hemerocallis plantlets fortuitously generated in vitro.
Daylily plantlets generated on semi-solid media from morphogenetically competent cells or morphogenetically competent cells regenerated from protoplasts can give rise in aseptic culture to plantlets with a mature phenotype.  The individual leaves of these plantlets open to the extreme base so that no encircling leaf sheath is present.  This permits the overlapping bases and leaves to assume an open fan-like arrangement.  The occurrence of fans correlates with exceptionally tightly sealed culture vessels and experiments to date suggest a gaseous component is associated with this change of growth form.  It has not been possible to fix the mature growth mode, however, and new leaf growth assumes the more normal juvenile phenotype when the gaseous environment is altered by admitting or exposure to room air.
Fitter
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Department of Biochemistry, State University of New York at Stony Brook 11794, USA.
Krikorian
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D003470:Culture Media; D046508:Culture Techniques / Q000379:methods; D010641:Phenotype; D059828:Plant Cells; D063245:Plant Development; D018515:Plant Leaves / Q000166:cytology / Q000254:growth & development* / Q000502:physiology; D018521:Plant Physiological Phenomena
D003470:Culture Media