Lymphocyte collection for donor leucocyte infusion from normal donors: estimation of the minimum processed blood volume and safety of the procedure.
OBJECTIVE
To estimate the minimum volume of processed blood necessary for the purpose of donor leucocyte infusion (DLI), we determined the number of CD3+ cells harvested by apheresis from normal donors and examined adverse events during the procedure.


METHODS
Leukapheresis utilizing the COBE Spectra' was performed a total of 24 times from 12 normal donors. Blood counts were obtained and the number of CD3+ cells was evaluated before and after apheresis and in the peripheral blood products. Complications associated with the procedure were documented.


RESULTS
Blood products contained, on average, 51.1% CD3+ cells. A linear correlation was found between the number of CD3+ cells collected and the processed blood volume, up to 12 000 ml (r = 0.930, P < 0.001). Cytoreduction was observed in all donors after apheresis. In particular, the platelet and lymphocyte values decreased to approximately 70% of the preapheresis value. Two donors reported adverse effects: one was a mild vaso-vagal reaction and the other was citrate-related lip paresthesia. These symptoms were mild and disappeared spontaneously.


CONCLUSIONS
We found a linear correlation between the number of CD3+ cells collected and the processed blood volume, which enabled us to estimate the minimum volume of processed blood necessary for DLI. The adverse events observed were acceptable.
Sato
H
H
Division of Transfusion Medicine, Kanazawa University Hospital, Kanazawa, Ishikawa, Japan. hsatoh@med.kanazawa-u.ac.jp
Shiobara
S
S
Yasue
S
S
Chuhjo
T
T
Nakao
S
S
D000293:Adolescent; D000328:Adult; D001772:Blood Cell Count; D001782:Blood Donors; D001810:Blood Volume; D017252:CD3 Complex / Q000032:analysis; D005260:Female; D006801:Humans; D007937:Leukapheresis / Q000295:instrumentation / Q000379:methods / Q000592:standards*; D017710:Lymphocyte Transfusion; D008297:Male; D008875:Middle Aged; D012044:Regression Analysis
D017252:CD3 Complex