Utilization of chicory roots for microbial endoinulinase production.
OBJECTIVE
The optimal culture conditions for endoinulinase production using chicory roots were studied in shake-flask culture.


RESULTS
Much higher enzyme production was achieved with Xanthomonas sp. (15 U ml(-1)) than with Pseudomonas sp. (3 U ml(-1)). Optimized culture conditions of Xanthomonas sp. for endoinulinase production in flask culture were: chicory powder, 5 g l(-1); temperature, 37 degrees C; pH, 7.0; agitation speed, 100 rev min(-1).


CONCLUSIONS
Maximum bacterial growth and enzyme production were 6.2 g l(-1) and 20 U ml(-1) under optimal conditions, respectively.


CONCLUSIONS
Chicory roots could be used as a fermentation substrate for the production of enndoinulinase.
Park
J P
JP
Department of Biotechnology, Taegu University, Kyungsan, Kyungbuk 712-714, Korea.
Yun
J W
JW
D018651:Cichorium intybus / Q000378:metabolism*; D003470:Culture Media; D005285:Fermentation; D006026:Glycoside Hydrolases / Q000096:biosynthesis*; D006863:Hydrogen-Ion Concentration; D007444:Inulin / Q000378:metabolism; D018517:Plant Roots / Q000378:metabolism*; D011549:Pseudomonas / Q000201:enzymology* / Q000254:growth & development; D013696:Temperature; D014974:Xanthomonas / Q000201:enzymology* / Q000254:growth & development
D003470:Culture Media; D007444:Inulin; D006026:Glycoside Hydrolases; C023086:inulinase