DNA analysis for the Dombrock polymorphism.
BACKGROUND
RBC typing for Do(a) and Do(b) is notoriously difficult, and inaccurate typing can predispose patients to hemolytic transfusion reactions. The DO1/DO2 polymorphism is associated with three nucleotide changes: 378 C>T, 624 T>C and 793 A>G. While the 378 C>T- and 624 T>C-containing codons are silent mutations, the 793 A>G polymorphism in codon 265 encodes asparagine for Do(a) and aspartic acid for Do(b).


METHODS
Described here are two PCR-RFLP assays, one using the Mnl I site associated with 624C (DO2) and the other altering two nucleotides within the sense primer, which allows recognition of 793G by the Eam 1105 I.


RESULTS
The assays have been performed on over 100 samples for which the RBC typing of one or both antigens was known. Eight samples had been historically mistyped by hemagglutination.


CONCLUSIONS
This RFLP assay provides a practical method for typing donor blood for Dombrock alleles.
Rios
M
M
Immunochemistry Laboratory, New York Blood Center, New York, New York 10021, USA.
Hue-Roye
K
K
Lee
A H
AH
Chiofolo
J T
JT
Miller
J L
JL
Reid
M E
ME
D001483:Base Sequence / Q000235:genetics; D001782:Blood Donors; D001789:Blood Group Antigens / Q000235:genetics*; D001788:Blood Grouping and Crossmatching; D004247:DNA / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length
D001789:Blood Group Antigens; D004247:DNA