Grafting of encapsulated genetically modified cells secreting GDNF into the striatum of parkinsonian model rats.
In order to deliver glial cell line-derived neurotrophic factor (GDNF) into the brain, we have established a cell line that produces GDNF in a continuous fashion by genetic engineering. These cells were encapsulated and grafted into parkinsonian model rats that had received unilateral intrastriatal injection of 6-hydroxydopamine 2 weeks earlier. Neurochemical analysis showed that GDNF has been produced from the capsule for 6 months after grafting and histological analysis revealed good survival of GDNF-producing cells in the capsule 6 months after grafting. The density of nigrostriatal dopaminergic fibers in the striatum as well as the number of dopaminergic cell bodies in the substantia nigra recovered significantly after GDNF-producing cell grafting. These results suggest the possible application of GDNF-producing cell grafting for the treatment of Parkinson's disease.
Date
I
I
Department of Neurological Surgery, Okayama University Medical School, Japan. idate333@med.okayama-u.ac.jp
Shingo
T
T
Yoshida
H
H
Fujiwara
K
K
Kobayashi
K
K
Takeuchi
A
A
Ohmoto
T
T
D000818:Animals; D002214:Capsules; D002460:Cell Line; D017690:Cell Transplantation / Q000379:methods*; D006224:Cricetinae; D004195:Disease Models, Animal; D004298:Dopamine / Q000378:metabolism; D005818:Genetic Engineering; D051100:Glial Cell Line-Derived Neurotrophic Factor; D006801:Humans; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D009474:Neurons / Q000737:chemistry / Q000378:metabolism*; D009483:Neuropsychological Tests; D020734:Parkinsonian Disorders / Q000628:therapy*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014162:Transfection; D014183:Transplantation, Heterologous; D014793:Visual Cortex / Q000166:cytology / Q000378:metabolism / Q000601:surgery
D002214:Capsules; C495039:GDNF protein, human; C495041:Gdnf protein, rat; D051100:Glial Cell Line-Derived Neurotrophic Factor; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D004298:Dopamine