Large veins in the Atlantic cod (Gadus morhua) and the rainbow trout (Oncorhynchus mykiss) are innervated by neuropeptide-containing nerves.
Previous studies have shown that fish veins are reactive to several hormones known to exist in the fish circulation. Besides this humoral control, another possible means of active regulation of venous return is by autonomic nervous control of venous tone. This study therefore investigated the presence of a perivascular innervation of major veins in the Atlantic cod (Gadus morhua) and the rainbow trout (Oncorhynchus mykiss) using immunohistochemical methods. Histological staining was employed to investigate the smooth muscle distribution in the vessel walls. Vasoactive intestinal polypeptide-immunoreactive nerve fibers were found to be widespread in the venous system of G. morhua and O. mykiss, while pituitary adenylate cyclase-activating polypeptide-immunoreactive fibers were demonstrated in the duct of Cuvier of both species. Fibers containing neurokinin A and/or substance P were found in the duct of Cuvier and the posterior cardinal vein of both species and in the hepatic portal vein of O. mykiss. Calcitonin-gene related peptide-immunoreactive fibers were present in the duct of Cuvier of both species and in the hepatic portal vein of O. mykiss. Galanin-immunoreactive fibers were found in the duct of Cuvier in O. mykiss and in the hepatic portal vein of both species. Co-existence of neuropeptides in the perivascular nerve fibers was investigated by double labelling. Vasoactive intestinal polypeptide/pituitary adenylate cyclase-activating polypeptide-immunoreactive fibers were found in both species. Vasoactive intestinal polypeptide/galanin-immunoreactive fibers and vasoactive intestinal polypeptide/calcitoningene related peptide-immunoreactive fibers were found in G. morhua but not in O. mykiss. This study gives further evidence for an active venoregulation by autonomic nerves in teleost fish.
Johnsson
M
M
Department of Zoology, Göteborg University, Sweden. m.johnsson@zool.gu.se
Axelsson
M
M
Holmgren
S
S
D000818:Animals; D001341:Autonomic Nervous System / Q000166:cytology*; D015740:Calcitonin Gene-Related Peptide / Q000032:analysis; D019004:Galanin / Q000032:analysis; D009131:Muscle, Smooth, Vascular / Q000294:innervation; D009412:Nerve Fibers / Q000737:chemistry / Q000201:enzymology; D015288:Neurokinin A / Q000032:analysis; D009479:Neuropeptides / Q000032:analysis*; D019001:Nitric Oxide Synthase / Q000032:analysis; D017686:Oncorhynchus mykiss / Q000033:anatomy & histology*; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D013373:Substance P / Q000032:analysis; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis; D014660:Vasoactive Intestinal Peptide / Q000032:analysis; D014680:Veins / Q000294:innervation*
D009479:Neuropeptides; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D013373:Substance P; D014660:Vasoactive Intestinal Peptide; D015288:Neurokinin A; D019004:Galanin; D019001:Nitric Oxide Synthase; D014446:Tyrosine 3-Monooxygenase; D015740:Calcitonin Gene-Related Peptide