The action of starch synthase II on 6"'-alpha-maltotriosyl-maltohexaose comprising the branch point of amylopectin.
The principle of using a chemically synthesized, well-defined branched oligosaccharide to provide a more detailed knowledge of the substrate specificity of starch synthase II (SSII) is demonstrated. The branched nonasaccharide, 6"'-alpha-maltotriosyl-maltohexaose, was investigated as a primer for particulate SSII using starch granules prepared from the low-amylose pea mutant lam as the enzyme source. The starch granule preparation from the lam pea mutant contains no starch synthases other than SSII and is devoid of alpha-amylase, beta-amylase and phosphorylase activity. SSII was demonstrated to catalyse a specific nonprocessive elongation of the nonreducing end of the shortest unit chain of 6"'-alpha-maltotriosyl-maltohexaose, i.e. the maltotriose chain. Maltotriose and maltohexaose, representing the two linear building units of the branched nonasaccharide, were also tested as primers for SSII. Maltotriose was elongated more efficiently than 6"'-alpha-maltotriosyl-maltohexaose and maltohexaose was used less efficiently. Compared to the surface exposed alpha-glucan chains of the granule bound amylopectin molecules, all three soluble oligosaccharides tested were poor primers for SSII. This indicates that in vivo, the soluble oligosaccharides supposedly released as result of amylopectin trimming reactions are not re-introduced into starch biosynthetic reactions via the action of the granule bound fraction of SSII.
Damager
I
I
Plant Biochemistry Laboratory, Department of Plant Biology, The Royal Veterinary and Agricultural University, Copenhagen, Denmark.
Denyer
K
K
Motawia
M S
MS
Møller
B L
BL
Blennow
A
A
D000687:Amylopectin / Q000737:chemistry* / Q000378:metabolism*; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D003594:Cytoplasmic Granules / Q000201:enzymology; D005947:Glucose / Q000378:metabolism; D009154:Mutation; D009844:Oligosaccharides / Q000737:chemistry / Q000378:metabolism*; D018532:Pisum sativum / Q000166:cytology / Q000201:enzymology* / Q000235:genetics; D010940:Plant Proteins; D012995:Solubility; D013214:Starch Synthase / Q000378:metabolism*; D013379:Substrate Specificity; D014312:Trisaccharides / Q000378:metabolism
C400079:6'''-alpha-maltotriosyl-maltohexaose; D009844:Oligosaccharides; D010940:Plant Proteins; D014312:Trisaccharides; C016549:maltohexaose; C008317:maltotriose; D000687:Amylopectin; C111377:starch synthase II; D013214:Starch Synthase; D005947:Glucose