Lipid content and fatty acid composition of green algae Scenedesmus obliquus grown in a constant cell density apparatus.
The lipids of alga Scenedesmus obliquus grown under controlled conditions were separated and fractionated by column and thin-layer chromatography, and fatty acid composition of each lipid component was studied by gas-liquid chromatography (GLC).  Total lipids were 11.17%, and neutral lipid, glycolipid and phospholipid fractions were 7.24%, 2.45% and 1.48% on a dry weight basis, respectively.  The major neutral lipids were diglycerides, triglycerides, free sterols, hydrocarbons and sterol esters.  The glycolipids were:  monogalactosyl diglyceride, digalactosyl diglyceride, esterified sterol glycoside, and sterol glycoside.  The phospholipids included:  phosphatidyl choline, phosphatidyl glycerol and phosphatidyl ethanolamine.  Fourteen fatty acids were identified in the four lipid fractions by GLC.  The main fatty acids were C18:2, C16:0, C18:3(alpha), C18:1, C16:3, C16:1, and C16:4.  Total unsaturated fatty acid and essential fatty acid compositions of the total algal lipids were 80% and 38%, respectively.
Choi
K J
KJ
Department of Applied Biological Sciences, Massachusetts Institute of Technology, Cambridge 02139, USA.
Nakhost
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Z
Barzana
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Karel
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D000460:Chlorophyta / Q000737:chemistry* / Q000254:growth & development; D002849:Chromatography, Gas / Q000379:methods; D002855:Chromatography, Thin Layer / Q000379:methods; D004780:Environment, Controlled; D005227:Fatty Acids / Q000032:analysis* / Q000302:isolation & purification*; D006017:Glycolipids / Q000032:analysis; D008055:Lipids / Q000032:analysis* / Q000302:isolation & purification*; D010743:Phospholipids / Q000032:analysis
D005227:Fatty Acids; D006017:Glycolipids; D008055:Lipids; D010743:Phospholipids