Unhydroxylated triple helical collagen I produced in transgenic plants provides new clues on the role of hydroxyproline in collagen folding and fibril formation.
Human unhydroxylated homotrimeric triple-helical collagen I produced in transgenic plants was used as an experimental model to provide insights into the role of hydroxyproline in molecular folding and fibril formation. By using chemically cross-linked molecules, we show here that the absence of hydroxyproline residues does not prevent correct folding of the recombinant collagen although it markedly slows down the propagation rate compared with bovine fully hydroxylated homotrimeric collagen I. Relatively slow cis-trans-isomerization in the absence of hydroxyproline likely represents the rate-limiting factor in the propagation of the unhydroxylated collagen helix. Because of the lack of hydroxylation, recombinant collagen molecules showed increased flexibility as well as a reduced melting temperature compared with native homotrimers and heterotrimers, whereas the distribution of charged amino acids was unchanged. However, unlike with bovine collagen I, the recombinant collagen did not self-assemble into banded fibrils in physiological ionic strength buffer at 20 degrees C. Striated fibrils were only obtained with low ionic strength buffer. We propose that, under physiological ionic strength conditions, the hydroxyl groups in the native molecule retain water more efficiently thus favoring correct fibril formation. The importance of hydroxyproline in collagen self-assembly suggested by others from the crystal structures of collagen model peptides is thus confirmed experimentally on the entire collagen molecule.
Perret
S
S
Institut de Biologie et Chimie des Protéines, CNRS UMR 5086, Université Claude Bernard Lyon I, 7 Passage du Vercors, F-69367 Lyon Cedex 07, France.
Merle
C
C
Bernocco
S
S
Berland
P
P
Garrone
R
R
Hulmes
D J
DJ
Theisen
M
M
Ruggiero
F
F
D000818:Animals; D002417:Cattle; D024042:Collagen Type I / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D006909:Hydroxyproline / Q000502:physiology*; D030821:Plants, Genetically Modified / Q000235:genetics*; D011487:Protein Conformation; D017510:Protein Folding
D024042:Collagen Type I; D006909:Hydroxyproline