title: Allosteric phenomena in cytochrome P450-catalyzed monooxygenations.
abstract: Allosteric regulation of monooxygenase activity is shown to occur with diverse cytochrome P450 isoforms and is characterized by kinetic patterns deviating from the Michaelis-Menten model. Homotropic and heterotropic phenomena are encountered in both substrate activation and productive coupling of the electron donors NADPH-cytochrome P450 reductase and cytochrome b5, and the lipid environment of the system also appears to play a role as an effector. Circumstantial analysis reveals the components of the electron transfer chain to be mutually beneficial in interactions with each other depending on the substrate used and type of cytochrome P450 operative. It is noteworthy that association of diatomic gaseous ligands may be amenable to allosteric regulation as well. Thus, dioxygen binding to cytochrome P450 displays nonhyperbolic kinetic profiles in the presence of certain substrates; the latter, together with redox proteins such as cytochrome b5, can exert efficient control of the abortive breakdown of the oxyferrous intermediates formed. Similarly, substrates may modulate the structural features of the access channel for solutes such as carbon monoxide in specific cytochrome P450 isozymes to either facilitate or impair ligand diffusion to the heme iron. The in vivo importance of allosteric regulation of enzyme activity is discussed in detail.
authors.affiliation: Walther-Straub-Institut für Pharmakologie und Toxikologie der LMU, München, Germany. Hlavica@llrz.uni-muenchen.de
authors.forename: P
authors.initials: P
authors.lastname: Hlavica
authors.forename: D F
authors.initials: DF
authors.lastname: Lewis
mesh_terms: D000494:Allosteric Regulation; D000818:Animals; D001665:Binding Sites; D002248:Carbon Monoxide / Q000378:metabolism; D002384:Catalysis; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D004579:Electron Transport; D006801:Humans; D050356:Lipid Metabolism; D010100:Oxygen / Q000378:metabolism*; D011485:Protein Binding
chemical_list: D002248:Carbon Monoxide; D003577:Cytochrome P-450 Enzyme System; D010100:Oxygen