DNA polymerase beta imbalance increases apoptosis and mutagenesis induced by oxidative stress.
Oxidative stress has been proposed to be one of the major causes leading to the accumulation of mutation that is associated with the initiation and progression of cancers. Elevated expression of DNA polymerase beta, an event found in many human tumors, has been shown to generate a mutator phenotype. Here, we demonstrated that overexpression of DNA polymerase beta strengthens the mutagenicity of oxidative damages, concomitantly with a higher cellular sensitivity and increased apoptosis. Deregulated expression of DNA polymerase beta could represent a predisposition factor for mutagenic effects of oxidative stress and thus have implication in the generation and/or evolution of cancer.
Fréchet
M
M
IPBS-CNRS UMR 5089, groupe Instabilité Génétique et Cancer, 205 route de Narbonne, 31077 Toulouse Cedex, France.
Canitrot
Y
Y
Cazaux
C
C
Hoffmann
J S
JS
D000818:Animals; D017209:Apoptosis; D016466:CHO Cells; D006224:Cricetinae; D019951:DNA Polymerase beta / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D006861:Hydrogen Peroxide / Q000378:metabolism; D016296:Mutagenesis; D009154:Mutation; D018384:Oxidative Stress; D010100:Oxygen / Q000378:metabolism; D010641:Phenotype; D017382:Reactive Oxygen Species / Q000378:metabolism; D013997:Time Factors; D014162:Transfection
D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide; D019951:DNA Polymerase beta; D010100:Oxygen