title: 1-[2-methoxy-5-(3-phenylpropyl)]-2-aminopropane unexpectedly shows 5-HT(2A) serotonin receptor affinity and antagonist character.
abstract: Certain phenylethylamines, such as 1-(4-bromo-2,5-dimethoxyphenyl)-2-aminopropane (DOB; 1a), are high-affinity 5-HT(2) agonists. Previous structure-affinity studies have concluded that both the 2,5-dimethoxy substitution pattern and the nature of substituents at the 4-position are important determinants of high affinity. We recently demonstrated that replacement of the bromo group of DOB with a 3-(phenyl)propyl substituent results in retention of affinity and that, counter to established structure-affinity relationships, the 2,5-dimethoxy substitution pattern is no longer a requirement for the binding. The present investigation extends these findings by examining a series of analogues, 3, lacking a 5-methoxy group. It was additionally found that shifting the phenylalkyl substituent from the 4- to the 5-position (e.g., 4i) also results in retention of affinity. For example, 1-(2-methoxy-5-(3-phenylpropyl)-2-aminopropane (6; the alpha-methyl derivative of 4i) binds at 5-HT(2A) receptors with high affinity (K(i) = 13 nM) and possesses 5-HT(2A) antagonist character. Thus, not only is the 2,5-dimethoxy substitution pattern not a requirement for the binding of certain phenylethylamines at 5-HT(2A) receptors, the presence of a 4-position substituent (previously thought to serve as a modulator of affinity of DOB-like agents) is also not required. Striking differences in the 5-HT(2A) binding requirements of the present compounds as compared to DOB-like agents suggest multiple substituent-dependent modes of binding.
authors.affiliation: Department of Medicinal Chemistry, School of Pharmacy, Virginia Commonwealth University, Richmond, VA 23298-0540, USA.
authors.forename: J B
authors.initials: JB
authors.lastname: Rangisetty
authors.forename: M
authors.initials: M
authors.lastname: Dukat
authors.forename: C S
authors.initials: CS
authors.lastname: Dowd
authors.forename: K
authors.initials: K
authors.lastname: Herrick-Davis
authors.forename: A
authors.initials: A
authors.lastname: DuPre
authors.forename: S
authors.initials: S
authors.lastname: Gadepalli
authors.forename: M
authors.initials: M
authors.lastname: Teitler
authors.forename: C R
authors.initials: CR
authors.lastname: Kelley
authors.forename: N A
authors.initials: NA
authors.lastname: Sharif
authors.forename: R A
authors.initials: RA
authors.lastname: Glennon
mesh_terms: D016475:3T3 Cells; D000818:Animals; D002478:Cells, Cultured; D006868:Hydrolysis; D007295:Inositol Phosphates / Q000378:metabolism; D051379:Mice; D008958:Models, Molecular; D009131:Muscle, Smooth, Vascular / Q000166:cytology; D011437:Propylamines / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D011869:Radioligand Assay; D051381:Rats; D044402:Receptor, Serotonin, 5-HT2A; D044405:Receptor, Serotonin, 5-HT2C; D011985:Receptors, Serotonin / Q000378:metabolism*; D012702:Serotonin Antagonists / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D013329:Structure-Activity Relationship
chemical_list: C437309:1-(2-methoxy-5-(3-phenylpropyl))-2-aminopropane; D007295:Inositol Phosphates; D011437:Propylamines; D044402:Receptor, Serotonin, 5-HT2A; D044405:Receptor, Serotonin, 5-HT2C; D011985:Receptors, Serotonin; D012702:Serotonin Antagonists