Genetic and molecular characterization of a variegating hsp70-acZ fusion gene in the euchromatic 31 B region of Drosophila melanogaster.
A hsp70-lacZ fusion gene introduced into Drosophila melanogaster at the euchromatic 31B region by Pelement transformation displayed a variegated expression with respect to the lacZ fusion protein in the salivary gland cells under heat-shock conditions. The variegation is also reflected by the chromosome puffing pattern. Subsequent transposition of the 31B P element to other euchromatic positions restored wild-type activity, that is, a nonvariegated phenotype. A lower developmental temperature reduced the amount of expression under heat-shock conditions, similar to genes undergoing position-effect variegation (PEV). However, other modifiers of PEV did not affect the expression pattern of the gene. These results show a novel euchromatic tissue-specific variegation that is not associated with classical heterochromatic PEV.
Morcillo
P
P
Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA.
MacIntyre
R J
RJ
D000818:Animals; D015139:Blotting, Southern; D002875:Chromosomes / Q000648:ultrastructure; D003360:Cosmids / Q000378:metabolism; D004331:Drosophila melanogaster / Q000235:genetics*; D004742:Enhancer Elements, Genetic; D015723:Gene Library; D017930:Genes, Reporter; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*; D006570:Heterochromatin / Q000378:metabolism; D017403:In Situ Hybridization; D007763:Lac Operon / Q000235:genetics*; D008957:Models, Genetic; D010641:Phenotype; D012469:Salivary Glands / Q000166:cytology; D013489:Suppression, Genetic; D013696:Temperature; D001616:beta-Galactosidase / Q000378:metabolism
D018840:HSP70 Heat-Shock Proteins; D006570:Heterochromatin; D001616:beta-Galactosidase