p38: A novel protein that associates with the vesicular stomatitis virus glycoprotein.
The vesicular stomatitis virus glycoprotein (VSV G) is a model transmembrane glycoprotein that has been extensively used to study the exocytotic pathway. The cytoplasmic domain of VSV G contains information for several intracellular sorting steps including efficient export from the ER, basolateral delivery, and endocytosis. In order to identify proteins that potentially interact with the polypeptide sorting motifs in the VSV G tail, the carboxy-terminal 27 amino acids of VSV G were used as bait in a yeast two-hybrid system. The protein identified most frequently in the screen is a novel protein of 38 kDa, p38. In the present work, the initial molecular and biochemical characterization of p38 is described. Preliminary evidence suggests that p38 may interact transiently with endoplasmic reticulum (ER) membranes, and thus may affect VSV G and other cargo movement at the step of ER to Golgi traffic.
Sevier
C S
CS
Department of Cell Biology, Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, Maryland 21205, USA.
Machamer
C E
CE
D000595:Amino Acid Sequence; D000906:Antibodies; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism; D018076:DNA, Complementary / Q000032:analysis / Q000302:isolation & purification; D006367:HeLa Cells; D006801:Humans; D008562:Membrane Glycoproteins; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D013347:Subcellular Fractions; D014018:Tissue Distribution; D020798:Two-Hybrid System Techniques; D014759:Viral Envelope Proteins / Q000378:metabolism*; D048051:p38 Mitogen-Activated Protein Kinases
D000906:Antibodies; D002352:Carrier Proteins; D018076:DNA, Complementary; C028391:G protein, vesicular stomatitis virus; D008562:Membrane Glycoproteins; D014759:Viral Envelope Proteins; D048051:p38 Mitogen-Activated Protein Kinases