Induction of cyclosporin A-sensitive pore in mitochondria of intact neurons during uncoupling of oxidative phosphorylation.
Using primary cultures of cerebellar granule cells we showed that Ca(2+)transported into neurons under the effect of glutamate is accumulated and stored in mitochondria for a long time. Protonophore FCCP, an uncoupler of oxidative phosphorylation, stimulated the release of Ca(2+)from mitochondria in a calcium-free medium in 81% glutamate-treated cells. Cyclosporin A and ATP-synthase blocker oligomycin decreased the number of cells with FCCP-induced Ca(2+)release to 53 and 12%, respectively. Oligomycin partly prevented glutamate- and FCCP-induced decrease of intracellular ATP level.
Storozhevykh
T P
TP
Laboratory of Membranology, Center of Children Health, Russian Academy of Medical Sciences, Moscow. tstor@mail.ru
Yuryavichyus
A I
AI
Sorokina
E G
EG
Pinelis
V G
VG
D000255:Adenosine Triphosphate / Q000096:biosynthesis / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000378:metabolism / Q000494:pharmacology; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone / Q000494:pharmacology; D002478:Cells, Cultured; D016572:Cyclosporine / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D018698:Glutamic Acid / Q000494:pharmacology; D008928:Mitochondria / Q000187:drug effects*; D025261:Mitochondrial Proton-Translocating ATPases / Q000037:antagonists & inhibitors; D009474:Neurons / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism*; D010766:Phosphorylation; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D014475:Uncoupling Agents / Q000494:pharmacology
D004791:Enzyme Inhibitors; D014475:Uncoupling Agents; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone; D018698:Glutamic Acid; D016572:Cyclosporine; D000255:Adenosine Triphosphate; D025261:Mitochondrial Proton-Translocating ATPases; D010100:Oxygen; D002118:Calcium