Application of dipolar coupling data to the refinement of the solution structure of the sarcin-ricin loop RNA.
Residual dipolar couplings can provide the long-range information that most NMR solution structures lack. The use of such data in protein structure determinations is now fairly routine, but even though these data should be much more useful for nucleic acids, their application to nucleic acid structure determination is still in its infancy. Here we present a method for producing accurate, dipolar-refined structures of nucleic acids that is more efficient than those used previously, and apply it to E73, a 29 nucleotide RNA that includes the sarcin-ricin loop from rat 28S rRNA. The results enable us to address the differences between the crystal structure of E73 and the solution structure proposed for it previously.
Warren
J J
JJ
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520-8107, USA.
Moore
P B
PB
D000818:Animals; D008958:Models, Molecular; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D009690:Nucleic Acid Conformation; D012313:RNA / Q000737:chemistry*; D051381:Rats; D012276:Ricin / Q000737:chemistry / Q000235:genetics*; D014961:X-Ray Diffraction
D012313:RNA; D012276:Ricin