Heparin-binding proteins of human seminal plasma homologous with boar spermadhesins.
Protein homologues to boar seminal plasma spermadhesins with the N-terminal sequence AQN (AQN spermadhesins) and with the N-terminal sequence AWN (AWN spermadhesins) were detected in human seminal plasma and characterized. They were isolated as heparin-binding (HB) proteins from human seminal plasma by affinity chromatography on heparin-Sepharose and then separated into 12 fractions (HB1-HB12) by RP HPLC or into four major fractions (HB-I-HB-IV) by gel filtration. Rabbit antibody against boar seminal plasma AQN 1 spermadhesin cross-reacted with 10-14 kDa proteins of fraction HB7, and antibody against AWN 1 spermadhesin cross-reacted with 11-14 kDa proteins of fractions HB9 and HB11. Both antibodies interacted with 10-14 kDa proteins in fractions HB-I and HB-II. The N-terminal amino acid sequence (1)AQNKG(5)... was determined in the 14 kDa protein of fraction HB-I cross-reacting with AQN 1 antibodies. A component detected among 10-14 kDa proteins of HB7 cross-reacting with rabbit antiserum against AQN 1 had the N-terminal sequence (1)GELKFVTLVFAVGDYE(16), which is similar to the sequence of a fragment of prostatic acid phosphatase. Lactoferrin and its fragments were immunodetected with rabbit antibody against human milk lactoferrin in fractions HB7-HB11. This was proved by N-terminal sequencing of a lactoferrin fragment immunodetected in fraction HB7. N-terminal amino acid sequence analysis of the dominant component of fraction HB2 revealed the presence of a fragment of semenogelin I.
Kraus
M
M
Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Flemingovo nám. 2, 166 37 Prague 6, Czech Republic. kraus@img.cas.cz
Tichá
M
M
Jonáková
V
V
D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D002352:Carrier Proteins / Q000737:chemistry / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D006493:Heparin / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding; D011506:Proteins / Q000302:isolation & purification / Q000378:metabolism*; D012661:Semen / Q000737:chemistry*; D029589:Seminal Plasma Proteins; D017386:Sequence Homology, Amino Acid; D013552:Swine
D002352:Carrier Proteins; D010446:Peptide Fragments; D011506:Proteins; D029589:Seminal Plasma Proteins; C425042:spermadhesin; D006493:Heparin