title: Promotion of triplex formation by 2'-O,4'-C-methylene bridged nucleic acid (2',4'-BNA) modification: thermodynamic and kinetic studies.
abstract: We analyzed the effect of 2'-O,4'-C-methylene bridged nucleic acid (2',4'-BNA) modification of triplex-forming oligonucleotide (TFO) on pyrimidine motif triplex formation at neutral pH, a condition where pyrimidine motif triplexes are unstable. The binding constant of the pyrimidine motif triplex formation at pH 6.8 with 2',4'-BNA modified TFO was about 20 times larger than that observed with unmodified TFO. The observed increase in the binding constant at neutral pH by the 2',4'-BNA modification resulted from the considerable decrease in the dissociation rate constant.
authors.affiliation: Tsukuba Life Science Center, Institute of Physical and Chemical Research (RIKEN), 3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Torigoe
authors.forename: S
authors.initials: S
authors.lastname: Obika
authors.forename: T
authors.initials: T
authors.lastname: Imanishi
mesh_terms: D004247:DNA / Q000737:chemistry*; D006838:Hydrocarbons; D007700:Kinetics; D008697:Methane / Q000031:analogs & derivatives* / Q000737:chemistry; D009841:Oligonucleotides / Q000737:chemistry*; D011742:Pyrimidine Nucleotides / Q000737:chemistry*; D013816:Thermodynamics
chemical_list: D006838:Hydrocarbons; D009841:Oligonucleotides; D011742:Pyrimidine Nucleotides; C051802:triplex DNA; C030011:carbene; D004247:DNA; D008697:Methane