In vivo antiviral activity of Stephania cepharantha against herpes simplex virus type-1.
The antiviral activity of a MeOH extract of Stephania cepharantha (root tubers), its CHCl3-soluble fraction (alkaloid fraction) and the major alkaloid FK-3000 (1) were investigated in BALB/c mice cutaneously infected with HSV-1 strain 7401H. At doses of 125 and 250 mg/kg body weight, p.o., the MeOH extract significantly delayed skin lesion on score 2 (vesicles in the local region), limited the development of further lesions on score 6 (mild zosteriform lesion) and prolonged the mean survival time of HSV-1 infected mice. After p.o. administration of the CHCl3-soluble fraction at doses of 25 and 50 mg/kg or FK-3000 (1) at 10 and 25 mg/kg, similar results were obtained. Although the alkaloid improved the survival of infected mice, it had a narrow therapeutic index.
Nawawi
A
A
Institute of Natural Medicine, Toyama Medical and Pharmaceutical University, 2630 Sugitani, Toyama, 930-0194, Japan.
Nakamura
N
N
Meselhy
M R
MR
Hattori
M
M
Kurokawa
M
M
Shiraki
K
K
Kashiwaba
N
N
Ono
M
M
D000470:Alkaloids / Q000737:chemistry / Q000627:therapeutic use* / Q000633:toxicity; D000818:Animals; D000998:Antiviral Agents / Q000737:chemistry / Q000627:therapeutic use* / Q000633:toxicity; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000627:therapeutic use* / Q000633:toxicity; D005260:Female; D029001:Herbal Medicine; D006561:Herpes Simplex / Q000188:drug therapy*; D019684:Magnoliopsida / Q000737:chemistry*; D008516:Medicine, Chinese Traditional; D051379:Mice; D008807:Mice, Inbred BALB C; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000627:therapeutic use* / Q000633:toxicity; D018517:Plant Roots / Q000737:chemistry; D018514:Plant Structures; D018139:Simplexvirus / Q000187:drug effects*
D000470:Alkaloids; D000998:Antiviral Agents; D004365:Drugs, Chinese Herbal; C442856:FK3000; D010936:Plant Extracts