Mapping protein-protein interactions with combinatorial biology methods.
Extremely diverse, DNA-encoded libraries of peptides and proteins have been constructed that include a linkage between each polypeptide and the encoding DNA. Library members can be selected by virtue of a particular binding specificity, and their protein sequence can be deduced from the sequence of the cognate DNA. Such combinatorial biology methods have proven invaluable in both identifying natural protein-protein interactions and also in mapping the specificities and energetics of these interactions in fine detail.
Pelletier
J
J
Université de Montréal, Département de Chimie, 2900 Edouard-Montpetit, Montréal, Québec H3C 3J7, Canada. joelle.pelletier@umontreal.ca
Sidhu
S
S
D000595:Amino Acid Sequence / Q000235:genetics; D001483:Base Sequence / Q000235:genetics; D020650:Combinatorial Chemistry Techniques; D004247:DNA / Q000235:genetics; D019151:Peptide Library; D011485:Protein Binding; D011506:Proteins / Q000235:genetics* / Q000378:metabolism*; D012680:Sensitivity and Specificity
D019151:Peptide Library; D011506:Proteins; D004247:DNA