title: Novel motility mutants of synechocystis strain PCC 6803 generated by in vitro transposon mutagenesis.
abstract: We screened for transposon-generated mutants of Synechocystis sp. strain PCC 6803 that exhibited aberrant phototactic movement. Of the 300 mutants generated, about 50 have been partially characterized; several contained transposons in genes encoding chemotaxis-related proteins, while others mapped to novel genes. These novel genes and their possible roles in motility are discussed.
authors.affiliation: Department of Plant Biology, Carnegie Institution, Stanford, California 94305, USA. devaki@andrew2.stanford.edu
authors.forename: D
authors.initials: D
authors.lastname: Bhaya
authors.forename: A
authors.initials: A
authors.lastname: Takahashi
authors.forename: P
authors.initials: P
authors.lastname: Shahi
authors.forename: A R
authors.initials: AR
authors.lastname: Grossman
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics; D000595:Amino Acid Sequence; D000458:Cyanobacteria / Q000502:physiology*; D004251:DNA Transposable Elements; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D018832:Molecular Chaperones / Q000235:genetics; D008969:Molecular Sequence Data; D009068:Movement / Q000502:physiology*; D016254:Mutagenesis, Insertional; D009154:Mutation; D017386:Sequence Homology, Amino Acid
chemical_list: D018528:ATP-Binding Cassette Transporters; D004251:DNA Transposable Elements; D029968:Escherichia coli Proteins; D018840:HSP70 Heat-Shock Proteins; D018832:Molecular Chaperones; C063842:dnaK protein, E coli