Production of the biocontrol agent Pantoea agglomerans strain CPA-2 using commercial products and by-products.
The aim of this paper was to find the nitrogen and carbon sources that provide maximum biomass production of strain CPA-2 of the biocontrol agent Pantoea agglomerans and minimum cost of media, whilst maintaining biocontrol efficacy. To reduce the cost of media, commercial products and by-products were tested. P. agglomerans can be produced using a combination of nitrogen sources such as yeast extract (5 g l(-1)) and dry beer yeast (10 g l(-1)) with inexpensive carbohydrates such as sucrose (10 g l(-1)) and molasses (20 g l(-1)), respectively, maintaining the efficacy of the biocontrol agent against Penicillium digitatum and P. italicum on oranges. The results obtained in this study could be used to provide a reliable basis for a scale-up of this fermentation process to an industrial level.
Costa
E
E
Postharvest Unit, CeRTA, Centre UdL-IRTA, Lleida, Spain. elena.costa@irta.es
Teixidó
N
N
Usall
J
J
Atarés
E
E
Viñas
I
I
D018533:Biomass; D001709:Biotechnology / Q000379:methods; D002244:Carbon / Q000378:metabolism; D002957:Citrus / Q000382:microbiology*; D003470:Culture Media / Q000191:economics; D009584:Nitrogen / Q000378:metabolism; D020636:Pantoea / Q000254:growth & development*; D010407:Penicillium / Q000254:growth & development*; D010572:Pest Control, Biological; D010935:Plant Diseases / Q000382:microbiology*
D003470:Culture Media; D002244:Carbon; D009584:Nitrogen