Synthesis of modified nucleoside 5'-triphosphates for in vitro selection of catalytic nucleic acids.
2'-Modified pyrimidine nucleoside 5'-triphosphates comprising amino, imidazole and carboxylate functionality attached to the 5-position of the base were synthesized. Two different phosphorylation methods were used to optimize the yields of these highly modified triphosphates.
Matulic-Adamic
J
J
Department of Chemistry & Biochemistry, Ribozyme Pharmaceuticals, Inc., 2950 Wilderness Place, Boulder, Colorado 80301, USA.
Daniher
A T
AT
Karpeisky
A
A
Haeberli
P
P
Sweedler
D
D
Beigelman
L
L
D004355:Drug Stability; D011742:Pyrimidine Nucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D016337:RNA, Catalytic / Q000737:chemistry*
D011742:Pyrimidine Nucleotides; D016337:RNA, Catalytic