title: The use and evaluation of 2 + 2 photoaddition in immobilization of oligonucleotides on a three dimensional hydrogel matrix.
abstract: Photochemical attachment of synthetic oligonucleotides on the three dimensional surface of a polyacrylamide based hydrogel was used in the specific detection of target oligonucleotides. Covalent attachment of the oligonucleotide to the hydrogel was mediated by the incorporation of a 2 + 2 photo-attachable functional group in both the hydrogel and the oligonucleotide probe. Expression and SNP assays were used to evaluate this platform.
authors.affiliation: Motorola BioChip Systems, Northbrook, Illinois, USA.
authors.forename: R
authors.initials: R
authors.lastname: Elghanian
authors.forename: Y
authors.initials: Y
authors.lastname: Xu
authors.forename: J
authors.initials: J
authors.lastname: McGowen
authors.forename: M
authors.initials: M
authors.lastname: Seithoff
authors.forename: C G
authors.initials: CG
authors.lastname: Liu
authors.forename: J
authors.initials: J
authors.lastname: Winick
authors.forename: N
authors.initials: N
authors.lastname: Fuller
authors.forename: R
authors.initials: R
authors.lastname: Ramakrishnan
authors.forename: A
authors.initials: A
authors.lastname: Beuhler
authors.forename: T
authors.initials: T
authors.lastname: Johnson
authors.forename: A
authors.initials: A
authors.lastname: Mazumder
authors.forename: C K
authors.initials: CK
authors.lastname: Brush
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D020411:Oligonucleotide Array Sequence Analysis; D015345:Oligonucleotide Probes / Q000737:chemistry; D009841:Oligonucleotides / Q000737:chemistry*; D010777:Photochemistry; D000099166:Polyacrylamides
chemical_list: D000180:Acrylic Resins; D020100:Hydrogels; D015345:Oligonucleotide Probes; D009841:Oligonucleotides; D000099166:Polyacrylamides