Differentiation of human acute myeloid leukaemia cells in primary culture in response to cotylenin A, a plant growth regulator.
Cotylenin A, which has a diterpenoid tricarbocyclic skeleton, has been isolated as a plant growth regulator, has been shown to affect several physiological processes of higher plants and have differentiation-inducing activity in several myeloid leukaemia cell lines. We examined the effect of cotylenin A on the differentiation of leukaemic cells that were freshly isolated from acute myeloid leukaemia (AML) patients in primary culture. Cotylenin A significantly stimulated both functional and morphological differentiation of leukaemia cells in 9 out of 12 cases. This differentiation-inducing activity was more potent than those of all-trans retinoic acid and 1alpha,25-dihydroxyvitamin D3 (VD3). Treatment with a combination of cotylenin A and VD3 was more effective than cotylenin A or VD3 alone at inducing the monocytic differentiation of AML cells.
Yamada
K
K
Department of Haematology, Showa University School of Medicine, Tokyo, Japan.
Honma
Y
Y
Asahi
K I
KI
Sassa
T
T
Hino
K I
KI
Tomoyasu
S
S
D000208:Acute Disease; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015703:Antigens, CD / Q000276:immunology; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology; D002117:Calcitriol / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D004224:Diterpenes / Q000494:pharmacology*; D004357:Drug Synergism; D005260:Female; D006801:Humans; D037181:Lectins, C-Type; D007951:Leukemia, Myeloid / Q000276:immunology / Q000628:therapy*; D018950:Lipopolysaccharide Receptors / Q000276:immunology; D016177:Macrophage-1 Antigen / Q000276:immunology; D008297:Male; D008875:Middle Aged; D010937:Plant Growth Regulators / Q000494:pharmacology*; D018709:Statistics, Nonparametric; D014212:Tretinoin / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects; D000099192:CD69 Antigens
D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D002117:Calcitriol; D004224:Diterpenes; D037181:Lectins, C-Type; D018950:Lipopolysaccharide Receptors; D016177:Macrophage-1 Antigen; D010937:Plant Growth Regulators; D014212:Tretinoin; D000099192:CD69 Antigens; C423222:cotylenin A