[Effect of developing Sarcocystis muris tissue cysts on ultrastructural change of murine muscle fibers].
A study was made of the influence exerted by developing sarcocysts of Sarcocystis muris on the ultrastructural organization of muscle fibres, both harbouring the sarcocysts (HSM) and sarcocyst-free (SFM), from skeletal muscles of experimentally infected mice. Muscle fibres of non-infected mice of the same age served as a control. Mice were sacrificed 6 months following feeding S. muris oocysts (or sporocysts). The developing sarcocysts seriously destroyed HSM: their myofilaments were no hold in register, cross-bridges almost entirely disappeared, M-lines and Z-disks looked as broken structures. The majority of actin myofilaments were arranged along myosin myofilaments as discrete units. The host cell sarcoplasm was packed with numerous vacuoles of different form and size. Compared to muscle fibres in the control, SFM of infected mice also displayed an obvious ultrastructural alteration. On the periphery of SFM, some destroyed sarcomeres with swollen myofilaments were noticed whose cross-bridges were totally lacking. In other extreme areas myosin and actin myofilaments were disintegrated into thin straightened filaments 2.0-2.5 nm in diameter. It is supposed that HSM and SFM of the infected mice may experience different kinds of influence on the part of the developing intracellular parasite (sarcocyst). And it dos not seem unlikely that various biologically active substances, produced by the parasite, may be vesicle transported to SFN through the endomysium space.
Radchenko
A I
AI
Institute of Cytology RAS, St. Petersburg. rai@cit.ras.spb.ru
Beĭer
T V
TV
D000818:Animals; D051379:Mice; D008854:Microscopy, Electron; D018482:Muscle, Skeletal / Q000469:parasitology / Q000473:pathology / Q000648:ultrastructure*; D012522:Sarcocystis / Q000472:pathogenicity*; D012523:Sarcocystosis / Q000473:pathology*