[Level of retinol and beta-carotene in plasma of smoking and non-smoking women].
Cigarette smoking during pregnancy generates many oxidants and free radicals and it has been implicated in cellular damage. Retinol and its provitamin beta-carotene are natural factors protecting cells from damaging influence of free oxygen species. The aim of our study was the assessment of vitamin A and beta-carotene levels in plasma of smoking and non-smoking pregnant women. Assignment the groups was based on the concentration of serum cotinine. In the presented studies the mean level of serum cotinine in smoking pregnant women was 1326 +/- 790 micrograms/l (range 350-1930 micrograms/l). In the group of tobacco abstinents the level of cotinine was in a range of 0-60 micrograms/l. Plasma concentrations of retinol and beta-carotene were measured by high pressure liquid chromatography (HPLC). Plasma level of vitamin A was lowered by 20% and was 1.64 +/- 0.42 mumol/l and 2.06 +/- 0.58 mumol/l in smoking and abstinent groups respectively. Women who smoke during pregnancy also have significantly lower levels of beta-carotene in plasma (1.9 +/- 0.5 mumol/l) than non-smokers (2.4 +/- 0.6 mumol/l; p < 0.001). We have observed that plasma concentration of beta-carotene has decreased since 21 gestation week in smokers while in abstinent group increased during pregnancy. The increase of plasma level of beta-carotene indicates that their physiological requirement is satisfied by standard vitamins supplementation. The reduced concentrations of plasma retinol and beta-carotene suggest that consumption of these antioxidants for neutralization of free radicals present in cigarette smoking is enhanced. The observed changes indicate that vitamins intake in smoking pregnant women should be carefully controlled.
Chełchowska
M
M
Zakładu Biochemii Instytutu Matki i Dziecka w Warszawie. mchelchowska@yahoo.com
Laskowska-Klita
T
T
Szymborski
J
J
D000328:Adult; D003367:Cotinine / Q000097:blood; D005260:Female; D006801:Humans; D011247:Pregnancy; D012907:Smoking / Q000009:adverse effects / Q000097:blood*; D014801:Vitamin A / Q000097:blood*; D019207:beta Carotene / Q000097:blood*
D019207:beta Carotene; D014801:Vitamin A; D003367:Cotinine