title: Environmental signals controlling production of hemagglutinin/protease in Vibrio cholerae.
abstract: Vibrio cholerae hemagglutinin/protease (Hap) was induced upon nutrient limitation and strongly repressed by glucose. Hap was not produced in a mutant defective in the cyclic AMP (cAMP) receptor protein, suggesting that intracellular cAMP levels mediate Hap expression. No difference was found in Hap production between an rpoS deletion mutant and its isogenic wild-type precursor, indicating that the alternate sigma(s) factor is not essential for Hap expression. Based on these and previous results, we discuss the role of Hap in the pathogenesis of cholera.
authors.affiliation: California State University College of Science and Mathematics, Fresno, California 93740-8034, USA. jbenitez@csufresno.edu
authors.forename: J A
authors.initials: JA
authors.lastname: Benitez
authors.forename: A J
authors.initials: AJ
authors.lastname: Silva
authors.forename: R A
authors.initials: RA
authors.lastname: Finkelstein
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D050260:Carbohydrate Metabolism; D002244:Carbon / Q000378:metabolism; D003470:Culture Media; D000242:Cyclic AMP / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D008666:Metalloendopeptidases / Q000096:biosynthesis*; D011953:Receptors, Cyclic AMP / Q000235:genetics / Q000378:metabolism; D012808:Sigma Factor / Q000378:metabolism; D014734:Vibrio cholerae / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D011953:Receptors, Cyclic AMP; D012808:Sigma Factor; C069567:sigma factor KatF protein, Bacteria; D002244:Carbon; D000242:Cyclic AMP; D008666:Metalloendopeptidases; C039814:hemagglutinin-protease; D005947:Glucose