Sequence and genetic analyses of the 3' terminus and integration sites of the RIII/Sa mouse mammary tumor (MMTV) exogenous provirus.
The RIII/Sa mouse mammary tumor virus (MMTV) is one of several exogenous MMTV viruses transmitted to suckling mice through the milk. We report herein the nucleotide sequence of the exogenous RIII/Sa provirus from the central Eco RI site through to the end of the U5 region encoded by the 3' LTR. We also provide a detailed sequence analysis often different 3' terminal exogenous MMTV proviral integration sites within mammary tumor DNA obtained by the technique of genome walking. Using a combination of Southern blotting with 3' end probes and PCR utilizing a unique RIII/Sa specific 3' primer, we confirm that the RIII/Sa provirus integrates multiple times in mouse mammary tumors but that little or no integration occurs in various normal tissues. Southern blotting analyses with 3' end probes also indicate that RIII/Sa mice contain two additional endogenous MMTV loci, mtv-6 and mtv- 17, not previously reported. The combined genetic analyses reported herein distinguish between exogenous proviral integrants and endogenous germline MMTV.
Popken-Harris
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David F. Hickok Memorial Cancer Research Laboratory, Abbott Northwestern Hospital, the Minneapolis, MN 55407, USA.
Pliml
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Harris
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D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D015139:Blotting, Southern / Q000379:methods; D003001:Cloning, Molecular; D004279:DNA, Viral; D005260:Female; D015723:Gene Library; D005814:Genes, Viral; D008324:Mammary Tumor Virus, Mouse / Q000235:genetics* / Q000302:isolation & purification; D051379:Mice; D008969:Molecular Sequence Data; D011533:Proviruses / Q000235:genetics*; D017422:Sequence Analysis, DNA; D020079:Terminal Repeat Sequences; D016662:Virus Integration
D004279:DNA, Viral