The lin-11 LIM homeobox gene specifies olfactory and chemosensory neuron fates in C. elegans.
Chemosensory neuron diversity in C. elegans arises from the action of transcription factors that specify different aspects of sensory neuron fate. In the AWB and AWA olfactory neurons, the LIM homeobox gene lim-4 and the nuclear hormone receptor gene odr-7 are required to confer AWB and AWA-specific characteristics respectively, and to repress an AWC olfactory neuron-like default fate. Here, we show that AWA neuron fate is also regulated by a member of the LIM homeobox gene family, lin-11. lin-11 regulates AWA olfactory neuron differentiation by initiating expression of odr-7, which then autoregulates to maintain expression. lin-11 also regulates the fate of the ASG chemosensory neurons, which are the lineal sisters of the AWA neurons. We show that lin-11 is expressed dynamically in the AWA and ASG neurons, and that misexpression of lin-11 is sufficient to promote an ASG, but not an AWA fate, in a subset of neuron types. Our results suggest that differential temporal regulation of lin-11, presumably together with its interaction with asymmetrically segregated factors, results in the generation of the distinct AWA and ASG sensory neuron types. We propose that a LIM code may be an important contributor to the generation of functional diversity in a subset of olfactory and chemosensory neurons in C. elegans.
Sarafi-Reinach
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TR
Department of Biology and Volen Center for Complex Systems, Brandeis University, MS008, 415 South Street, Waltham, MA 02454, USA.
Melkman
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T
Hobert
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O
Sengupta
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P
D000818:Animals; D017173:Caenorhabditis elegans; D029742:Caenorhabditis elegans Proteins; D002454:Cell Differentiation; D019070:Cell Lineage; D005786:Gene Expression Regulation; D005801:Genes, Homeobox; D049452:Green Fluorescent Proteins; D015801:Helminth Proteins / Q000235:genetics; D018398:Homeodomain Proteins / Q000235:genetics / Q000502:physiology*; D007395:Interneurons / Q000378:metabolism; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D016296:Mutagenesis; D009419:Nerve Tissue Proteins / Q000235:genetics; D009474:Neurons / Q000166:cytology; D009475:Neurons, Afferent / Q000166:cytology*; D009832:Olfactory Nerve / Q000166:cytology*; D018035:Receptors, Odorant / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology
D029742:Caenorhabditis elegans Proteins; D015801:Helminth Proteins; D018398:Homeodomain Proteins; C111267:Lin-11 protein, C elegans; D008164:Luminescent Proteins; D009419:Nerve Tissue Proteins; D018035:Receptors, Odorant; D014157:Transcription Factors; C091156:odr-7 protein, C elegans; C415502:unc-130 protein, C elegans; D049452:Green Fluorescent Proteins