title: Complete, long-lasting protection against malaria of mice primed and boosted with two distinct viral vectors expressing the same plasmodial antigen.
abstract: We report that complete protection against malaria and total inhibition of liver stage development and parasitemia was obtained in 100% of BALB/c mice primed with a replication-defective recombinant adenovirus expressing the circumsporozoite (CS) protein of Plasmodium yoelii (AdPyCS), followed by a booster with an attenuated recombinant vaccinia virus, expressing the same malaria antigen, VacPyCS. We found increased levels of activated CS-specific CD8(+) and CD4(+) T cells, higher anti-sporozoite antibody titers, and greater protection in these mice, when the time between priming and boosting with these two viral vectors was extended from 2 to 8 or more weeks. Most importantly, by using this immunization regimen, the protection of the immunized mice was found to be long-lasting, namely complete resistance to infection of all animals 3 1/2 months after priming. These results indicate that immunization with AdPyCS generates highly effective memory T and B cells that can be recalled long after priming by boosting with VacPyCS.
authors.affiliation: Department of Medical and Molecular Parasitology, New York University School of Medicine, New York, NY 10010, USA.
authors.forename: O
authors.initials: O
authors.lastname: Bruña-Romero
authors.forename: G
authors.initials: G
authors.lastname: González-Aseguinolaza
authors.forename: J C
authors.initials: JC
authors.lastname: Hafalla
authors.forename: M
authors.initials: M
authors.lastname: Tsuji
authors.forename: R S
authors.initials: RS
authors.lastname: Nussenzweig
mesh_terms: D000818:Animals; D000917:Antibody Formation; D000962:Antimalarials / Q000627:therapeutic use; D001500:BCG Vaccine / Q000627:therapeutic use; D004195:Disease Models, Animal; D005355:Fibrosis; D006801:Humans; D007538:Isoniazid / Q000627:therapeutic use; D008252:Macaca fascicularis; D008253:Macaca mulatta; D009336:Necrosis; D013601:T-Lymphocytes / Q000276:immunology / Q000473:pathology; D014376:Tuberculosis / Q000276:immunology* / Q000473:pathology / Q000517:prevention & control
chemical_list: D000962:Antimalarials; D001500:BCG Vaccine; D007538:Isoniazid