Rapid and efficient syntheses of phosphorylated dinucleotides.
Nine or ten-step solution phase syntheses of the dimers dpApA, dApAp, dpTpT and dTpTp in 0.1-0.5 g amounts, in overall yields of 49%, 45%, 32%, and 20% respectively, are described. The synthetic intermediates were characterized by 1H and 31 P NMR and the structures of the final products were established by hydrolysis with alkaline phosphatase and comparison of the products with authentic samples.
Ferris
J P
JP
Department of Chemistry, Rensselaer Polytechnic Institute, Troy, NY 12180, USA.
Peyser
J R
JR
D002621:Chemistry / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D003854:Deoxyribonucleotides / Q000138:chemical synthesis*; D009682:Magnetic Resonance Spectroscopy; D009841:Oligonucleotides / Q000737:chemistry*; D010766:Phosphorylation
D003854:Deoxyribonucleotides; D009841:Oligonucleotides