title: Lipid peroxidation and polyamine metabolism in K562 cells subjected to altered gravity.
abstract: The mechanisms underlying cell sensitivity to the gravistimulus are still largely unknown. Recently, the oxidation of membrane lipids and the alteration of polyamine content have been shown to be involved in several cellular processes, from cell growth and differentiation, to aging and resistance to (a)biotic stress. Such an involvement was mediated by the modification of the activity and expression of lipoxygenase (LOX; E.C. 1.13.11.12) and diamine oxidase (DAO; E.C. 1.4.3.6), the enzymes which control membrane properties (LOX) and the catabolism of polyamines (DAO), respectively. In this study, the possible effects of altered gravity on membrane lipid peroxidation and polyamine metabolism were investigated, by subjecting human erythroleukemia K562 cell cultures to simulated hypogravity (by clinorotation) or hypergravity (by centrifugation).
authors.affiliation: Department of Experimental Medicine and Biochemical Sciences, University of Rome "Tor Vergata", Italy.
authors.forename: M
authors.initials: M
authors.lastname: Maccarrone
authors.forename: M
authors.initials: M
authors.lastname: Bari
authors.forename: A
authors.initials: A
authors.lastname: Finazzi Agro
mesh_terms: D006631:Amine Oxidase (Copper-Containing) / Q000378:metabolism; D002498:Centrifugation; D018470:Gravity, Altered; D006801:Humans; D018471:Hypergravity; D020014:K562 Cells / Q000201:enzymology / Q000378:metabolism*; D015227:Lipid Peroxidation / Q000502:physiology*; D008084:Lipoxygenase / Q000378:metabolism; D011073:Polyamines / Q000378:metabolism*; D011700:Putrescine / Q000378:metabolism; D012399:Rotation; D014407:Tumor Cells, Cultured
chemical_list: D011073:Polyamines; D008084:Lipoxygenase; D006631:Amine Oxidase (Copper-Containing); D011700:Putrescine