Solid-phase, single nucleotide primer extension of DNA/RNA hybrids by reverse transcriptases.
A method for RNA analysis based on primer extension by reverse transcriptase is described.
Pirrung
M C
MC
Department of Chemistry, Levine Science Research Center, Duke University, Durham, NC 27708-0317, USA. pirrung@chem.duke.edu
Worden
J D
JD
Labriola
J P
JP
Montague-Smith
M P
MP
Weislo
L J
LJ
D020137:Base Pair Mismatch; D004247:DNA / Q000737:chemistry*; D017931:DNA Primers / Q000737:chemistry*; D004259:DNA-Directed DNA Polymerase / Q000737:chemistry / Q000378:metabolism; D015336:Molecular Probe Techniques; D016133:Polymerase Chain Reaction; D012313:RNA / Q000737:chemistry*; D012194:RNA-Directed DNA Polymerase / Q000737:chemistry* / Q000378:metabolism
D017931:DNA Primers; D012313:RNA; D004247:DNA; C068508:Tth polymerase; D012194:RNA-Directed DNA Polymerase; D004259:DNA-Directed DNA Polymerase