title: Control of reaction chemoselectivity with a circular DNA template in the ligation of short oligodeoxyribonucleotides.
abstract: A study has been conducted to examine the chemoselective control attained in the ligation of short oligodeoxyribonucleotides (ODNs) directed by association on a circular DNA template through triplex formation relative to the same ligation on a single-strand DNA template through duplex formation. The highly efficient, chemospecific ligation achieved under a variety of conditions on the circular DNA template presumably arises from the higher substrate-template association resulting in improved transition state accessibility and protection of the ODNs from side reactions.
authors.affiliation: American Radiolabeled Corporation, St. Louis, MO 63146, USA.
authors.forename: J
authors.initials: J
authors.lastname: Selvasekaran
authors.forename: K D
authors.initials: KD
authors.lastname: Turnbull
mesh_terms: D002234:Carbodiimides / Q000737:chemistry; D004247:DNA; D004270:DNA, Circular / Q000378:metabolism*; D004277:DNA, Single-Stranded / Q000138:chemical synthesis; D006860:Hydrogen Bonding; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D013698:Templates, Genetic
chemical_list: D002234:Carbodiimides; D004270:DNA, Circular; D004277:DNA, Single-Stranded; D009838:Oligodeoxyribonucleotides; C051802:triplex DNA; D004247:DNA