title: Molecular analysis of mutagenesis by high LET radiation.
abstract: Mutation induction by high linear energy transfer [LET] alpha particles and gamma-rays was scored in the human hamster hybrid [AL] cells.  Southern blotting technique was used to analyse the molecular changes in the DNA from both the HGPRT- and S1- mutants.  Dose dependent mutagenesis in the AL cells irradiated with the charged particles was higher by almost 20 fold at the S1 than the corresponding HGPRT locus.  Southern analysis of the mutants induced by the high LET particles showed mostly multilocus deletion at both the HGPRT and S1 genes.
authors.affiliation: Center for Radiological Research, Columbia University, New York 10032, USA.
authors.forename: T K
authors.initials: TK
authors.lastname: Hei
authors.forename: L X
authors.initials: LX
authors.lastname: Zhu
authors.forename: D
authors.initials: D
authors.lastname: Vannais
authors.forename: C A
authors.initials: CA
authors.lastname: Waldren
mesh_terms: D000512:Alpha Particles; D000818:Animals; D000954:Antigens, Surface / Q000235:genetics / Q000528:radiation effects; D015139:Blotting, Southern; D016466:CHO Cells; D002460:Cell Line; D002470:Cell Survival / Q000528:radiation effects*; D002880:Chromosomes, Human, Pair 11 / Q000528:radiation effects; D003359:Cosmic Radiation; D006224:Cricetinae; D004249:DNA Damage; D018076:DNA, Complementary / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D005347:Fibroblasts; D005720:Gamma Rays; D017353:Gene Deletion; D006371:Helium; D006801:Humans; D006822:Hybrid Cells / Q000528:radiation effects*; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics* / Q000528:radiation effects; D018499:Linear Energy Transfer; D016296:Mutagenesis / Q000528:radiation effects*; D010315:Particle Accelerators; D012062:Relative Biological Effectiveness; D018570:Risk Assessment
chemical_list: D000954:Antigens, Surface; D018076:DNA, Complementary; D006371:Helium; D007041:Hypoxanthine Phosphoribosyltransferase