title: Mycobacterium bovis: polymerase chain reaction identification in bovine lymphonode biopsies and genotyping in isolates from Southeast Brazil by spolygotyping and restriction fragment length polymorphism.
abstract: Diagnosis of the Mycobacterium tuberculosis complex by direct PCR of mediastinal lymphnode DNA and microbiological tests were compared in cattle suspicious of bearing tuberculous-like lesions detected during slaughter. The PCR procedure applied on DNA samples (n=54) obtained by adding alpha -casein into the thiocyanate extraction mix was positive in 70% of the samples. PCR confirmed the identification of 23 samples (100%) that grew in culture, 9 samples (60%) that failed to grow in culture, plus 6 (37.5%) samples that resulted in growth of bacterial contaminants. Genotyping by IS6110-RFLP and DR-spoligotyping analysis of seven samples revealed the presence of several polimorphisms. Seven of the isolates contained multiple copies of IS6110, thus defining the existence of five singular genotypes.
authors.affiliation: Escola de Veterinária, ICB, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, MG, Brasil.
authors.forename: M S
authors.initials: MS
authors.lastname: Zanini
authors.forename: E C
authors.initials: EC
authors.lastname: Moreira
authors.forename: M T
authors.initials: MT
authors.lastname: Lopes
authors.forename: R S
authors.initials: RS
authors.lastname: Oliveira
authors.forename: S C
authors.initials: SC
authors.lastname: Leão
authors.forename: R L
authors.initials: RL
authors.lastname: Fioravanti
authors.forename: E
authors.initials: E
authors.lastname: Roxo
authors.forename: M
authors.initials: M
authors.lastname: Zumarraga
authors.forename: M I
authors.initials: MI
authors.lastname: Romano
authors.forename: A
authors.initials: A
authors.lastname: Cataldi
authors.forename: C E
authors.initials: CE
authors.lastname: Salas
mesh_terms: D000818:Animals; D002417:Cattle; D004269:DNA, Bacterial / Q000032:analysis; D005838:Genotype; D008198:Lymph Nodes / Q000382:microbiology*; D009163:Mycobacterium bovis / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D014380:Tuberculosis, Bovine / Q000382:microbiology
chemical_list: D004269:DNA, Bacterial