2'-modified oligonucleotides from methoxyoxalamido and succinimido precursors: synthesis, properties, and applications.
Synthesis of 2'-modified oligonucleotides from 2'-methoxyoxalamido (MOX) and 2'-succinimido (SUC) precursors is described. Their physical and biochemical properties were assessed. Synthesized oligonucleotides were used as primers in advanced DNA sequencing protocols. An example of sequencing directly off genomic DNA template without prior cloning or PCR amplification is presented.
Polushin
N
N
Fidelity Systems, Inc., 7961 Cessna Avenue, Gaithersburg, Maryland 20879-4117, USA.
Malykh
A
A
Malykh
O
O
Zenkova
M
M
Chumakova
N
N
Vlassov
V
V
Kozyavkin
S
S
D001483:Base Sequence; D017931:DNA Primers / Q000138:chemical synthesis* / Q000737:chemistry; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D010072:Oxamic Acid / Q000031:analogs & derivatives* / Q000737:chemistry; D010766:Phosphorylation; D017422:Sequence Analysis, DNA / Q000379:methods; D013388:Succinimides / Q000737:chemistry*
D017931:DNA Primers; D009841:Oligonucleotides; D013388:Succinimides; D010072:Oxamic Acid