Immunohistochemical detection of lipid peroxidation products, protein-bound acrolein and 4-hydroxynonenal protein adducts, in actinic elastosis of photodamaged skin.
Acrolein and 4-hydroxy-2-nonenal (HNE) are both byproducts of a lipid peroxidation reaction. Actinic elastosis in photodamaged skin of aged individuals is characterized by the accumulation of fragmented elastic fibers in the sun-exposed areas. To study whether a lipid peroxidation reaction is involved in the accumulation of altered elastic fibers in actinic elastosis, skin specimens obtained from sun-damaged areas were immunohistochemically examined using the antibodies against acrolein and HNE. Both antibodies were found to react with the accumulations of elastic material. Double immunofluorescence labeling demonstrated that acrolein/elastin and HNE/elastin were colocalized in the actinic elastosis. Western blot analysis showed that the polypeptide with a molecular weight of 62 kDa reacted with anti-acrolein, anti-HNE and anti-elastin antibodies. The results suggest that acrolein and HNE may be associated with actinic elastosis.
Tanaka
N
N
Department of Dermatology, National Defense Medical College, Tokorozawa, Saitama, Japan.
Tajima
S
S
Ishibashi
A
A
Uchida
K
K
Shigematsu
T
T
D000171:Acrolein / Q000378:metabolism*; D000368:Aged; D000369:Aged, 80 and over; D000447:Aldehydes / Q000378:metabolism*; D004547:Elastic Tissue / Q000378:metabolism; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008054:Lipid Peroxides / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D010787:Photosensitivity Disorders / Q000209:etiology* / Q000378:metabolism* / Q000473:pathology; D015595:Skin Aging / Q000502:physiology*; D013960:Thyroid Function Tests; D014018:Tissue Distribution
D000447:Aldehydes; D008054:Lipid Peroxides; D000171:Acrolein; C027576:4-hydroxy-2-nonenal