title: Structural determination of an exocellular mannan from Rhodotorula mucilaginosa YR-2 using ab initio assignment of proton and carbon NMR spectra.
abstract: The structure of the title mannan was determined exclusively by NMR. Because of the short relaxation time of the native mannan (100 kDa), a partially hydrolyzed mannan (10 kDa) was used for proton assignments by COSY, to correlate proton and carbon signals by HMQC, and to determine linkage positions between residues by HMBC. A further hydrolyzed mannan (oligomers of approximately 1.5 kDa) was used to determine the anomeric configuration, using Wilker's quasi-3D method [Wilker, W.; Leibfritz, D. Magn. Reson. Chem. 1995, 33, 632-638]. The procedure presented here can be used to determine the structure of any polysaccharide.
authors.affiliation: Nitto Best Co. Ltd., Sagae 991-8610, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Takita
authors.forename: S
authors.initials: S
authors.lastname: Katohda
authors.forename: H
authors.initials: H
authors.lastname: Sugiyama
mesh_terms: D002247:Carbon Isotopes; D008351:Mannans / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular; D011522:Protons; D012248:Rhodotorula / Q000737:chemistry*
chemical_list: D002247:Carbon Isotopes; D008351:Mannans; D011522:Protons