Using rapid freeze and freeze-substitution for the preparation of yeast cells for electron microscopy and three-dimensional analysis.
Giddings
T H
TH
Department of Molecular, Cellular and Developmental Biology, Porter Biosciences, University of Colorado-Boulder, Boulder, Colorado 80309, USA.
O'Toole
E T
ET
Morphew
M
M
Mastronarde
D N
DN
McIntosh
J R
JR
Winey
M
M
D005615:Freezing; D008854:Microscopy, Electron / Q000379:methods*; D016253:Microscopy, Immunoelectron / Q000379:methods*; D008870:Microtubules / Q000648:ultrastructure; D008938:Mitosis; D011312:Pressure; D012568:Schizosaccharomyces / Q000378:metabolism / Q000648:ultrastructure; D008941:Spindle Apparatus / Q000648:ultrastructure*; D013997:Time Factors