Skeletogenesis in sea urchin larvae under modified gravity conditions.
From many points of view, skeletogenesis in sea urchins has been well described. Based on this scientific background and considering practical aspects of sea urchin development (i.e. availability of material, size of larvae, etc.), we wanted to know whether orderly skeletogenesis requires the presence of gravity. The objective has been approached by three experiments successfully performed under genuine microgravity conditions (in the STS-65 IML-2 mission of 1994; in the Photon-10 IBIS mission of 1995 and in the STS-76 S/MM-03 mission of 1996). Larvae of the sea urchin Sphaerechinus granularis were allowed to develop in microgravity conditions for several days from blastula stage onwards (onset of skeletogenesis). At the end of the missions, the recovered skeletal structures were studied with respect to their mineral composition, architecture and size. Live larvae were also recovered for post-flight culture. The results obtained clearly show that the process of mineralisation is independent of gravity: that is, the skeletogenic cells differentiate correctly in microgravity. However, abnormal skeleton architectures were encountered, particularly in the IML-2 mission, indicating that the process of positioning of the skeletogenic cells may be affected, directly or indirectly, by environmental factors, including gravity. Larvae exposed to microgravity from blastula to prism/early pluteus stage for about 2 weeks (IBIS mission), developed on the ground over the next 2 months into normal metamorphosing individuals.
Marthy
H J
HJ
Observatoire Oceanologique, CNRS, Universite Pierre et Marie Curie, Banyuls sur mer, France.
Gasset
G
G
Tixador
R
R
Eche
B
B
Schatt
P
P
Dessommes
A
A
Marthy
U
U
Bacchieri
R
R
D000818:Animals; D002113:Calcification, Physiologic; D007814:Larva; D008648:Mesoderm / Q000502:physiology; D012617:Sea Urchins / Q000196:embryology / Q000254:growth & development*; D013026:Space Flight; D014893:Weightlessness