Identification and quantitation of 11-nor-delta9-tetrahydrocannabivarin-9-carboxylic acid, a major metabolite of delta9-tetrahydrocannabivarin.
After incubation of delta9-tetrahydrocannabivarin with human hepatocytes, a major metabolic product was detected by gas chromatography-mass spectrometry that showed identical retention time and mass spectrum to the synthetic 11-nor-delta9-tetrahydrocannabivarin-9-carboxylic acid (11-nor-delta9-THCV-9-COOH). Analysis of human urine specimens from marijuana users and plasma samples from Marinol users showed that 11-nor-delta9-THCV-9-COOH was only present in urine specimens of marijuana users. These results supported the conclusion that identification of 11-nor-delta9-THCV-9-COOH in a donor's urine specimen indicates the use or ingestion of cannabis-related product(s) and would not explain the sole use of Marinol.
ElSohly
M A
MA
ElSohly Laboratories, Incorporated, Oxford, Mississippi 38655, USA.
Feng
S
S
Murphy
T P
TP
Warrington
A W
AW
Ross
S
S
Nimrod
A
A
Mehmedic
Z
Z
Fortner
N
N
D002851:Chromatography, High Pressure Liquid; D013759:Dronabinol / Q000031:analogs & derivatives* / Q000032:analysis* / Q000652:urine*; D008401:Gas Chromatography-Mass Spectrometry; D006213:Hallucinogens / Q000652:urine*; D006801:Humans; D002189:Marijuana Abuse / Q000175:diagnosis*; D016482:Urinalysis
D006213:Hallucinogens; C003403:tetrahydrocannabivarin 9; D013759:Dronabinol