Immunochemical assay of glia-specific antigens as a criterion for blood-brain barrier permeability in rats during acute intoxication with sodium barbital.
Enzyme immunoassay showed penetration of two glia-specific antigens, glial fibrillar acid protein GFAP and specific brain glycoprotein alpha(2)GP, through the blood-brain barrier in rats treated with toxic doses of sodium barbital. The permeability of the blood-brain barrier was completely normalized 3 days after treatment. This method can be used in clinical practice for evaluation of the severity of impairment and dynamics of normalization of blood-brain barrier properties during acute intoxication with barbiturates.
Ryabukhin
I A
IA
V. P. Serbskii Research Center of Social and Forensic Medicine, Moscow.
Artemkina
I V
IV
Gurina
O I
OI
Sergienko
V I
VI
Chekhonin
V P
VP
D000818:Animals; D001463:Barbiturates / Q000633:toxicity; D001798:Blood Proteins / Q000096:biosynthesis; D001812:Blood-Brain Barrier / Q000502:physiology*; D002138:Calibration; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005904:Glial Fibrillary Acidic Protein / Q000097:blood; D006993:Hypnotics and Sedatives / Q000633:toxicity; D008297:Male; D009457:Neuroglia / Q000276:immunology*; D010424:Pentobarbital / Q000633:toxicity; D051381:Rats; D013997:Time Factors; D060749:alpha-2-HS-Glycoprotein
D001463:Barbiturates; D001798:Blood Proteins; D005904:Glial Fibrillary Acidic Protein; D006993:Hypnotics and Sedatives; D060749:alpha-2-HS-Glycoprotein; D010424:Pentobarbital