Viability of dielectrophoretically trapped neural cortical cells in culture.
Negative dielectrophoretic trapping of neural cells is an efficient way to position neural cells on the electrode sites of planar micro-electrode arrays. The preservation of viability of the neural cells is essential for this approach. This study investigates the viability of postnatal cortical rat cells that were dielectrophoretically trapped. Morphological characteristics as well as the ratio of the number of outgrowing to the number of non-outgrowing cortical cells were used to compare the viability of trapped cells to that of non-exposed cells. The morphological characteristics include the area of the cell, representing adhesive properties, and the number and length of the processes, as a measure for functional recovery. The results presented in this paper show that the viable state of dielectrophoretically trapped postnatal cortical rat cells under the conditions used was similar to that of non-exposed cells.
Heida
T
T
Faculty of Electrical Engineering, Institute for BioMedical Technology, University of Twente, PO Box 217, 7500 AE Enschede, The Netherlands. t.heida@el.utwente.nl
Vulto
P
P
Rutten
W L
WL
Marani
E
E
D000818:Animals; D000831:Animals, Newborn; D002448:Cell Adhesion / Q000502:physiology; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods*; D002454:Cell Differentiation / Q000502:physiology; D002463:Cell Membrane Permeability / Q000502:physiology; D048429:Cell Size / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured / Q000166:cytology / Q000502:physiology*; D002540:Cerebral Cortex / Q000166:cytology / Q000254:growth & development / Q000502:physiology; D004586:Electrophoresis / Q000295:instrumentation / Q000379:methods; D008839:Microelectrodes / Q000592:standards*; D016501:Neurites / Q000502:physiology / Q000648:ultrastructure; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D051381:Rats