Kinetics of photo-active bacteriorhodopsin analog 3,4-didehydroretinal.
Kinetics of the photo-induced processes of the transient states of the 3,4-didehydroretinal (3,4-dhr) modified bacteriorhodopsin (bR) was studied by a flash photolysis method in a water suspension at room temperature. The excitation initiated a photocycle with several transient intermediates similar to the trans photocycle of native bR. The main observation of the study was that although major part (80%) of the population of the M state relaxed via the O intermediate as in natural bR, 20% relaxed directly to the bR ground state in 200 ms.
Jussila
T
T
Institute of Materials Chemistry, Tampere University of Technology, P.O. Box 541, Tampere, Finland. tapio.jussila@tut.fi
Tkachenko
N V
NV
Parkkinen
S
S
Lemmetyinen
H
H
D001436:Bacteriorhodopsins / Q000737:chemistry*; D007700:Kinetics; D015394:Molecular Structure; D017319:Photosensitizing Agents / Q000737:chemistry*; D012172:Retinaldehyde / Q000031:analogs & derivatives / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D014801:Vitamin A / Q000031:analogs & derivatives* / Q000737:chemistry
D017319:Photosensitizing Agents; D014801:Vitamin A; D001436:Bacteriorhodopsins; C017184:dehydroretinal; D012172:Retinaldehyde