Demonstration by heavy-meromyosin of actin microfilaments in extracted cress (Lepidium sativum L.) root statocytes.
When statocytes of cress root-caps were incubated in an extraction medium containing phalloidin, bundles of microfilaments (6.6 +/- 2.4 nm in diameter) were found in the cortical cytoplasm. The inclusion of heavy-meromyosin in this medium demonstrated an arrowhead pattern on the filaments indicative of the presence of actin.
Hensel
W
W
Botanisches Institut der Universitat Bonn, Federal Republic of Germany.
D008841:Actin Cytoskeleton / Q000187:drug effects* / Q000502:physiology / Q000648:ultrastructure; D000199:Actins; D019607:Brassicaceae / Q000166:cytology / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure*; D004721:Endoplasmic Reticulum / Q000502:physiology / Q000648:ultrastructure; D015879:Myosin Subfragments / Q000494:pharmacology*; D010590:Phalloidine / Q000494:pharmacology*; D018518:Plant Root Cap / Q000187:drug effects / Q000254:growth & development / Q000648:ultrastructure*; D018087:Plastids / Q000187:drug effects* / Q000648:ultrastructure
D000199:Actins; D015879:Myosin Subfragments; D010590:Phalloidine