title: Occurrence of T-2 toxin in processed cereals and pulses in Turkey determined by HPLC and TLC.
abstract: The purpose of this study was to investigate the T-2 toxin level of contaminated cereal and pulse products in Turkey. T-2 toxin was detected using high performance liquid chromatography (HPLC) with UV detection at 208 nm and thin layer chromatography (TLC) was used for confirmation of the T-2 toxin-contaminated samples (> or = 1 ppm). The recovery was 93 +/- 3.3% (SD 3.29, n = 5) for chickpea spiked with a known amount of T-2 toxin (1 ppm). The detection limits for T-2 toxin for HPLC and TLC were 25 ng per injection and 50 ng per spot, respectively. A total of 50 commercially available cereal and pulse product samples, collected from markets and street bazaars, were analysed. Incidences of T-2 toxin detected in cereal and pulse products were 23.5% and 31.2%, respectively and the maximum detected amount was 1.9 ppm in a sample of dried beans. The incidence of toxin-contaminated specimens is not so low relative to the volume of specimens produced.
authors.affiliation: Department of Pharmaceutical Toxicology, Faculty of Pharmacy, Marmara University, Haydarpaş-Istanbul, Turkey. gomurtag@marmara.edu.tr
authors.forename: G Z
authors.initials: GZ
authors.lastname: Omurtag
authors.forename: D
authors.initials: D
authors.lastname: Yazicioğlu
mesh_terms: D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D002523:Edible Grain / Q000737:chemistry*; D007887:Fabaceae / Q000737:chemistry*; D005506:Food Contamination; D006801:Humans; D010946:Plants, Medicinal; D013605:T-2 Toxin / Q000032:analysis*; D014421:Turkey
chemical_list: D013605:T-2 Toxin