Synthesis of 3'-thioamido-modified 3'-deoxythymidine-5'-triphosphates and their use as chain terminators in Sanger-DNA sequencing.
The thioamide derivatives 3'-deoxy-5'-O-(4,4'-dimethoxytrityl)-3'-[(2-methyl-1-thioxo- propyl)amino]thymidine 1 and 3'-deoxy-5'-O-(4,4'-dimethoxytrityl)-3'-((6-([(9H-(fluo-ren-9- ylmethoxy)carbonyl]-amino)-1-thioxohexyl)amino) thymidine 2 were synthesized by regioselective thionation of their corresponding amides 7 and 8 with 2,4-bis(4-methoxyphenyl)-1,3,2,4-dithiadiphosphetane-2,4-disulfide (Lawesson's reagent). The thioamides were converted into the corresponding 5'-triphosphates 3 and 4. Compound 3 was chosen for DNA sequencing experiments and 4 was further labelled with fluorescein.
Schwarzer
K
K
Johann Wolfgang Goethe-Universität, Frankfurt am Main, Germany.
Wojczewski
C
C
Engels
J W
JW
D005452:Fluoresceins / Q000138:chemical synthesis / Q000737:chemistry; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry; D017422:Sequence Analysis, DNA / Q000379:methods; D013854:Thioamides / Q000138:chemical synthesis* / Q000737:chemistry; D013942:Thymine Nucleotides / Q000138:chemical synthesis* / Q000737:chemistry
D005452:Fluoresceins; D005456:Fluorescent Dyes; D013854:Thioamides; D013942:Thymine Nucleotides; C024157:thymidine 5'-triphosphate