title: Fluorescence in situ hybridization analysis of peripheral blood cells in Pearson marrow-pancreas syndrome.
abstract: We used a dual-color fluorescence in situ hybridization technique to estimate deleted mitochondrial DNA at a single-cell level and determine any correlation with the disease progression in lymphocytes from patients with Pearson marrow-pancreas syndrome. The method demonstrated a shift in heteroplasmy, paralleling the hematologic improvement.
authors.affiliation: Department of Environmental Medicine, Research Institute, Osaka Medical Center for Maternal and Child Health, Izumi City, Osaka, Japan.
authors.forename: I
authors.initials: I
authors.lastname: Yanagihara
authors.forename: K
authors.initials: K
authors.lastname: Inui
authors.forename: K
authors.initials: K
authors.lastname: Yanagihara
authors.forename: Y D
authors.initials: YD
authors.lastname: Park
authors.forename: J
authors.initials: J
authors.lastname: Tanaka
authors.forename: K
authors.initials: K
authors.lastname: Ozono
authors.forename: S
authors.initials: S
authors.lastname: Okada
authors.forename: H
authors.initials: H
authors.lastname: Kurahashi
mesh_terms: D000756:Anemia, Sideroblastic / Q000097:blood / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000097:blood*; D005260:Female; D006412:Hematopoietic Stem Cells / Q000473:pathology; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007223:Infant; D008214:Lymphocytes; D010182:Pancreatic Diseases / Q000097:blood / Q000235:genetics*; D013577:Syndrome
chemical_list: D004272:DNA, Mitochondrial