Cloning and characterization of hMAP126, a new member of mitotic spindle-associated proteins.
One novel gene product, hMAP126, was demonstrated to interact with p29 in the yeast two-hybrid assay. The full-length cDNA of hMAP126 has been obtained and encodes a protein of 1120 amino acids. Multiple tissue Northern blot analysis showed that hMAP126 was abundantly expressed in the testis. Polyclonal antiserum against hMAP126 was raised and affinity-purification of anti-hMAP126 antibodies was performed. The subcellular distribution of hMAP126 was localized to the mitotic spindle. Furthermore, hMAP126 was identified to be post-translationally modified and phosphorylated by p34(cdc2) kinase in vitro. Taken together, we have isolated a novel protein, hMAP126, which may be involved in the functional and dynamic regulation of mitotic spindles.
Chang
M S
MS
Department of Medical Research, Mackay Memorial Hospital, 45 Ming-San Road, Tamshui, Taipei, Taiwan.
Huang
C J
CJ
Chen
M L
ML
Chen
S T
ST
Fan
C C
CC
Chu
J M
JM
Lin
W C
WC
Yang
Y C
YC
D000595:Amino Acid Sequence; D016203:CDC2 Protein Kinase / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000032:analysis; D006367:HeLa Cells; D018398:Homeodomain Proteins / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational; D008941:Spindle Apparatus / Q000378:metabolism*; D013347:Subcellular Fractions; D014018:Tissue Distribution; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
D018797:Cell Cycle Proteins; D018076:DNA, Complementary; D018398:Homeodomain Proteins; C435984:PKNOX2 protein, human; C486665:Pknox2 protein, mouse; C486663:SPAG5 protein, human; D014157:Transcription Factors; D016203:CDC2 Protein Kinase