TGF-beta1 promotes liver metastasis of pancreatic cancer by modulating the capacity of cellular invasion.
We investigated the effect of TGF-beta1 on liver metastasis of pancreatic cancer using surgical specimens of pancreatic cancer and human pancreatic cancer cell lines Capan-2 and SW1990. Immunostaining of TGF-beta1 showed that TGF-beta1 positivity was significantly related to venous invasion and tumor staging, and also relatively associated with liver metastasis. Cellular invasion and protease production of MMP-2 and u-PA, and in vivo liver metastasis were significantly enhanced after treatment of cells with TGF-beta1. These findings suggest that TGF-beta1 might play an important role in enhancing liver metastasis of pancreatic cancer.
Teraoka
H
H
Department of Surgical Oncology (First Department of Surgery), Osaka City University Medical School, Osaka 545-8585, Japan. teraokk1@violin.ocn.ne.jp
Sawada
T
T
Yamashita
Y
Y
Nakata
B
B
Ohira
M
M
Ishikawa
T
T
Nishino
H
H
Hirakawa
K
K
D000230:Adenocarcinoma / Q000378:metabolism / Q000556:secondary*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D008113:Liver Neoplasms / Q000378:metabolism / Q000556:secondary*; D008297:Male; D020782:Matrix Metalloproteinases / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009367:Neoplasm Staging; D009368:Neoplasm Transplantation; D010190:Pancreatic Neoplasms / Q000378:metabolism / Q000473:pathology*; D016577:Pancreaticoduodenectomy; D016212:Transforming Growth Factor beta / Q000378:metabolism / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000187:drug effects* / Q000378:metabolism; D014568:Urokinase-Type Plasminogen Activator / Q000378:metabolism
D016212:Transforming Growth Factor beta; D014568:Urokinase-Type Plasminogen Activator; D020782:Matrix Metalloproteinases