Engineering preferences of hairpin PNA binding to complementary DNA: effect of N7G in aeg/aep PNA backbone.
AegPNA and aepPNA monomeric units bearing the N7-guanine nucleobase as a substitute for C+ have been demonstrated to bind to a GC base-pair of a duplex in a pH-independent manner when placed in the third strand. The aepPNA backbone exerts a preference for binding in the antiparallel Hoogsteen mode over the parallel Hoogsteen mode.
Kumar
V A
VA
Division of Organic Chemistry (Synthesis), National Chemical Laboratory, Pune, Maharashtra, India.
D'Costa
M
M
Ganesh
K N
KN
D018076:DNA, Complementary / Q000737:chemistry* / Q000378:metabolism; D006147:Guanine / Q000031:analogs & derivatives*; D006860:Hydrogen Bonding; D009690:Nucleic Acid Conformation; D020135:Peptide Nucleic Acids / Q000737:chemistry* / Q000378:metabolism; D011487:Protein Conformation; D011743:Pyrimidines / Q000737:chemistry / Q000378:metabolism
D018076:DNA, Complementary; D020135:Peptide Nucleic Acids; D011743:Pyrimidines; D006147:Guanine