[The role of the yolk sac in copper metabolism during rat embryogenesis].
Using the immunoblotting method, the synthesis of two copper-transporting P1-type ATPases, ATP7A (a candidate for the product of the Menkes disease gene) and ATP7B (presumed product of the Wilson disease gene), in the yolk sac cells of rat embryos at days 11 and 20 of embryogenesis was demonstrated. Concomitantly, yolk sac cells produce ceruloplasmin, a soluble copper-transporting glycoprotein, a proportion of which in secreted proteins progressively diminishes, attaining 5.2% at day 11 and 3.1% at day 20 of development. At different stages of embryogenesis, yolk sac cells synthesize two molecular forms of [14]C-ceruloplasmin, one of which is secreted towards the embryo, whereas the other, towards the decidual membrane. Two forms of ceruloplasmin secreted in polar directions differ in the rate of secretion. The role of the yolk sac as a key organ controlling the delivery and secretion of copper in the embryo during the postimplantation period is discussed.
Puchkova
L V
LV
Institute of Experimental Medicine, Russian Academy of Medical Sciences, ul. Akad. Pavlova 12, St. Petersburg, 197376 Russia.
Zhiguleva
C A
CA
Mokshina
S V
SV
Bichevaia
N K
NK
Platonova
N A
NA
Skvortsova
N N
NN
Sasina
L K
LK
Tsymbalenko
N V
NV
Chebotar'
N A
NA
Gaitshoki
V S
VS
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D027682:Cation Transport Proteins / Q000378:metabolism*; D002570:Ceruloplasmin / Q000378:metabolism*; D003300:Copper / Q000378:metabolism*; D000073840:Copper-Transporting ATPases; D005260:Female; D008969:Molecular Sequence Data; D011247:Pregnancy; D051381:Rats; D011993:Recombinant Fusion Proteins; D015017:Yolk Sac / Q000378:metabolism*
D002352:Carrier Proteins; D027682:Cation Transport Proteins; D011993:Recombinant Fusion Proteins; D003300:Copper; D002570:Ceruloplasmin; D000251:Adenosine Triphosphatases; C486202:Atp7a protein, rat; D000073840:Copper-Transporting ATPases