Novel concepts for selection of catalytic activity.
The selection of mutant enzymes with novel properties from libraries is emerging as a very powerful strategy for enzyme engineering. The past year has witnessed significant progress on several fronts: new and improved methods have been developed for the creation of libraries and advances have been made in screening and selection techniques. The results achieved demonstrate the enormous potential of the methods and leave questions open for further studies.
Soumillion
P
P
Laboratoire de Biochimie Physique et des Biopolymères, Institut des Sciences de la Vie, Université Catholique de Louvain, 1 Place Louis Pasteur, B 1348 Louvain la-Neuve, Belgium.
Fastrez
J
J
D002384:Catalysis; D019020:Directed Molecular Evolution / Q000379:methods*; D004353:Drug Evaluation, Preclinical / Q000379:methods; D004798:Enzymes / Q000235:genetics* / Q000378:metabolism*; D015723:Gene Library; D016296:Mutagenesis / Q000235:genetics; D019151:Peptide Library; D015202:Protein Engineering / Q000379:methods*
D004798:Enzymes; D019151:Peptide Library