Response to hypogravity of normal in vitro cultured follicular cells from thyroid.
Aim of this investigation is the study of molecular modifications occurring in differentiated mammalian cells exposed to gravitational changes. The test system chosen is a well characterized clone of differentiated, normal thyroid follicular cells (FRTL5) in long-term culture. As a follow-up to our recent experiment performed during the MASER-7 sounding rocket mission, flown for European Space Agency by Swedish Space Corporation in May 1996, we evaluated FRTL5 cells responses to Thyroid Stimulating Hormone dependent cAMP production under acute hypogravity conditions obtained in a fast rotating clinostat. Following this approach, we evaluated the FRTL5 cells response to TSH under microgravity conditions in order to optimize experimental tools and strategies in preparation to, and in between real flight missions.
Meli
A
A
Dipartimento di Patologia e Medicina Sperimentale e Clinica, Universita di Udine, Italy.
Perrella
G
G
Curcio
F
F
Ambesi-Impiombato
F S
FS
D000818:Animals; D002460:Cell Line; D000242:Cyclic AMP / Q000378:metabolism; D006112:Gravitation; D051381:Rats; D012399:Rotation; D013961:Thyroid Gland / Q000166:cytology* / Q000187:drug effects; D013972:Thyrotropin / Q000494:pharmacology*; D018474:Weightlessness Simulation
D013972:Thyrotropin; D000242:Cyclic AMP