title: Studies on phytanoyl-CoA 2-hydroxylase and synthesis of phytanoyl-coenzyme A.
abstract: Phytanoyl-CoA 2-hydroxylase (PAHX), an iron(II) and 2-oxoglutarate-dependent oxygenase, catalyses an essential step in the mammalian metabolism of beta-methylated fatty acids. Phytanoyl-CoA was synthesised and used to develop in vitro assays for PAHX. The product of the reaction was confirmed as 2-hydroxyphytanoyl-CoA by NMR and mass spectrometric analyses. In accord with in vivo analyses, hydroxylation of both 3R and 3S epimers of the substrate was catalysed by PAHX. Both pro- and mature- forms of PAHX were fully active.
authors.affiliation: The Oxford Centre for Molecular Sciences and The Dyson Perrins Laboratory, South Parks Road, OX1 3QY, Oxford, UK.
authors.forename: N J
authors.initials: NJ
authors.lastname: Kershaw
authors.forename: M
authors.initials: M
authors.lastname: Mukherji
authors.forename: C H
authors.initials: CH
authors.lastname: MacKinnon
authors.forename: T D
authors.initials: TD
authors.lastname: Claridge
authors.forename: B
authors.initials: B
authors.lastname: Odell
authors.forename: A S
authors.initials: AS
authors.lastname: Wierzbicki
authors.forename: M D
authors.initials: MD
authors.lastname: Lloyd
authors.forename: C J
authors.initials: CJ
authors.lastname: Schofield
mesh_terms: D003065:Coenzyme A / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D006899:Mixed Function Oxygenases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D010831:Phytanic Acid / Q000031:analogs & derivatives* / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization; D013237:Stereoisomerism
chemical_list: D011994:Recombinant Proteins; C095966:hydroxyphytanoyl-coenzyme A; C089917:phytanoyl-coenzyme A; D010831:Phytanic Acid; D006899:Mixed Function Oxygenases; C102623:PHYH protein, human; D003065:Coenzyme A