Mechanism of unassisted ion transport across membrane bilayers.
To establish how charged species move from water to the nonpolar membrane interior and to determine the energetic and structural effects accompanying this process, we performed molecular dynamics simulations of the transport of Na+ and Cl- across a lipid bilayer located between two water lamellae. The total length of molecular dynamics trajectories generated for each ion was 10 ns. Our simulations demonstrate that permeation of ions into the membrane is accompanied by the formation of deep, asymmetric thinning defects in the bilayer, whereby polar lipid head groups and water penetrate the nonpolar membrane interior. Once the ion crosses the midplane of the bilayer the deformation "switches sides"; the initial defect slowly relaxes, and a defect forms in the outgoing side of the bilayer. As a result, the ion remains well solvated during the process; the total number of oxygen atoms from water and lipid head groups in the first solvation shell remains constant. A similar membrane deformation is formed when the ion is instantaneously inserted into the interior of the bilayer. The formation of defects considerably lowers the free energy barrier to transfer of the ion across the bilayer and, consequently, increases the permeabilities of the membrane to ions, compared to the rigid, planar structure, by approximately 14 orders of magnitude. Our results have implications for drug delivery using liposomes and peptide insertion into membranes.
Wilson
M A
MA
Department of Pharmaceutical Chemistry, University of California, San Francisco 94143, USA.
Pohorille
A
A
D002463:Cell Membrane Permeability; D002713:Chlorine / Q000737:chemistry / Q000493:pharmacokinetics*; D003198:Computer Simulation; D004563:Electrochemistry; D005989:Glycerides / Q000737:chemistry; D017136:Ion Transport; D008051:Lipid Bilayers / Q000737:chemistry / Q000378:metabolism*; D008433:Mathematics; D008958:Models, Molecular; D012964:Sodium / Q000737:chemistry / Q000493:pharmacokinetics*; D014867:Water / Q000737:chemistry
D005989:Glycerides; D008051:Lipid Bilayers; D014867:Water; D002713:Chlorine; D012964:Sodium; C005953:monoolein