Multiple oligonucleotide synthesis in tandem on solid-phase supports for small and large scale synthesis.
Multiple oligonucleotides linked end-to-end in tandem can be synthesized by adding a nucleoside to the 5'-OH end of a prior sequence. Nucleosides with 3'-succinyl or Q-Linker arms are coupled with HBTU/DMAP. Alternatively, new phosphoramidite reagents with 3'-ester linkages can be used. Hydroxyl or amino supports can also be used as universal starting materials. Treatment with NH4OH cleaves the 3'-ester to yield only 3'-OH groups and no unwanted 3'-phosphorylated products occur.
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Dept. Biochem. & Molec. Biol., University of Calgary, Calgary, AB, Canada T2N 4N1.
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Sanghvi
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D003432:Cross-Linking Reagents / Q000138:chemical synthesis; D009705:Nucleosides / Q000737:chemistry; D009841:Oligonucleotides / Q000138:chemical synthesis*; D009943:Organophosphorus Compounds / Q000737:chemistry; D011725:Pyridines / Q000737:chemistry; D020080:Tandem Repeat Sequences
C417980:4-(dimethylamine)pyridine; D003432:Cross-Linking Reagents; D009705:Nucleosides; D009841:Oligonucleotides; D009943:Organophosphorus Compounds; D011725:Pyridines; C434331:phosphoramidite