Losses of chromosomal arms 1p and 19q in the diagnosis of oligodendroglioma. A study of paraffin-embedded sections.
Comparative genomic hybridization (CGH), fluorescence in situ hybridization (FISH), polymerase chain reaction-based microsatellite analysis, and p53 sequencing were performed in paraffin-embedded material from 18 oligodendrogliomas and histologically similar astrocytomas. The study was undertaken because of evidence that concurrent loss of both the 1p and 19q chromosome arms is a specific marker for oligodendrogliomas. Of the six lesions with a review diagnosis of oligodendroglioma, all had the predicted loss of 1p and 19q seen by CGH, FISH, and polymerase chain reaction. Other lesions, including some considered oligodendroglioma or mixed glioma by the submitting institution, did not. There were no p53 mutations in any of the six oligodendrogliomas, whereas 5 of the 10 remaining, successfully studied cases did have p53 mutations. The results suggest that CGH and FISH performed on current or archival tissue can aid in classification of infiltrating gliomas such as oligodendrogliomas and astrocytomas. The results of the p53 studies are consistent with findings of previous investigations that such mutations are less common in oligodendrogliomas than they are in astrocytomas.
Burger
P C
PC
The Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21287, USA. pburger@jhmi.edu
Minn
A Y
AY
Smith
J S
JS
Borell
T J
TJ
Jedlicka
A E
AE
Huntley
B K
BK
Goldthwaite
P T
PT
Jenkins
R B
RB
Feuerstein
B G
BG
D000328:Adult; D000368:Aged; D001932:Brain Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000378:metabolism; D002872:Chromosome Deletion; D002878:Chromosomes, Human, Pair 1 / Q000235:genetics*; D002888:Chromosomes, Human, Pair 19 / Q000235:genetics*; D004252:DNA Mutational Analysis; D004273:DNA, Neoplasm / Q000737:chemistry / Q000235:genetics; D003937:Diagnosis, Differential; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008867:Microtomy; D008875:Middle Aged; D009154:Mutation; D009693:Nucleic Acid Hybridization; D009837:Oligodendroglioma / Q000175:diagnosis / Q000235:genetics* / Q000378:metabolism; D016612:Paraffin Embedding; D016159:Tumor Suppressor Protein p53 / Q000032:analysis / Q000235:genetics
D004273:DNA, Neoplasm; D016159:Tumor Suppressor Protein p53