A calcium-selective optrode based on the fluorescence of dansylated troponin.
Troponin C (TnC) is covalently labeled with a fluorescent probe molecule dansylaziridine and immobilized on polyethyleneimine-impregnated cellulose.  An optrode is constructed by attaching the treated cellulose membrane to the distal end of a bifurcated fiber-optic cable.  Because fluorescence is enhanced as Ca+2 binds with the immobilized dansylated troponin (TnCDANZ), this system can be used as a calcium sensor for monitoring plasma Ca+2 levels.
Eckert-Tilotta
S E
SE
Department of Chemistry, Baylor University, Waco, Texas 76798.
Scouten
W H
WH
Hines
J
J
D015374:Biosensing Techniques; D002118:Calcium / Q000032:analysis / Q000378:metabolism*; D002482:Cellulose; D003619:Dansyl Compounds; D004867:Equipment Design; D005336:Fiber Optic Technology / Q000295:instrumentation / Q000379:methods; D005456:Fluorescent Dyes; D008567:Membranes, Artificial; D055100:Optical Fibers; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods; D014336:Troponin / Q000031:analogs & derivatives* / Q000737:chemistry*
D003619:Dansyl Compounds; D005456:Fluorescent Dyes; D008567:Membranes, Artificial; D014336:Troponin; C043338:dansylaziridine-troponin C; D002482:Cellulose; D002118:Calcium