Dehydroepiandrosterone (DHEA) stimulates glucose uptake in rat adipocytes: activation of phospholipase D.
We examined the effect of dehydroepiandrosterone (DHEA) on glucose uptake and phospholipase D (PLD) activation in rat adipocytes. DHEA (1 microM) provoked a twofold increase in [3H]2-deoxyglucose (DG) uptake for 30 min. Incorporation of [3H]glycerol into diacylglycerol was increased 150% above basal level for 20 min after stimulation with 1 microM DHEA. DHEA increased PLD activity, measured by the incorporation into [3H]phosphatidylethanol in [3H]palmitate labelled rat adipocytes, or by [3H]choline release in [methyl-(3)H]choline labeled rat adipocytes. Our results suggest that DHEA stimulates glucose uptake with activation of PLD in rat adipocytes.
Ishizawa
M
M
The Third Department of Internal Medicine, Department of General Medicine, Gifu University School of Medicine, Tsukasamachi 40, 500-8705, Gifu, Japan.
Ishizuka
T
T
Kajita
K
K
Miura
A
A
Kanoh
Y
Y
Kimura
M
M
Yasuda
K
K
D017667:Adipocytes / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002855:Chromatography, Thin Layer; D003687:Dehydroepiandrosterone / Q000494:pharmacology*; D003847:Deoxyglucose / Q000378:metabolism; D004075:Diglycerides / Q000096:biosynthesis / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D005947:Glucose / Q000378:metabolism*; D010739:Phospholipase D / Q000378:metabolism*; D051381:Rats; D013997:Time Factors
D004075:Diglycerides; D003687:Dehydroepiandrosterone; D003847:Deoxyglucose; D010739:Phospholipase D; D005947:Glucose