Characterisation of modified whey protein in milk ingredients by liquid chromatography coupled to electrospray ionisation mass spectrometry.
Whey proteins are an important ingredient in the food industry. We have investigated the protein composition of commercial whey samples by liquid chromatography coupled to electrospray ionisation mass spectrometry on a time-of-flight instrument. We found that industrial whey protein contains a multitude of different modifications, ranging from almost native proteins through different degrees of glycosylation and oxidation up to almost completely oxidised forms. The information obtained allows characterisation of the influence of industrial processing on protein modifications and classification of whey protein-based ingredients or milk powders from different suppliers.
Hau
J
J
Nestlé Research Center, Nestec Ltd., Lausanne, Switzerland. joerg.hau@rdls.nestle.com
Bovetto
L
L
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006031:Glycosylation; D008892:Milk / Q000737:chemistry*; D008894:Milk Proteins / Q000032:analysis*; D010084:Oxidation-Reduction; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D000067816:Whey Proteins
D008894:Milk Proteins; D000067816:Whey Proteins