title: Hormonal induction and antihormonal inhibition of tracheary element differentiation in Zinnia cell cultures.
abstract: Mechanically isolated mesophyll cells of Zinnia elegans L. cv Envy differentiate to tracheary elements when cultured in inductive medium containing sufficient auxin and cytokinin.  Tracheary element differentiation was induced by the three auxins (alpha-naphthaleneacetic acid, indole-3-acetic acid, and 2,4-dichlorophenoxyacetic acid) and four cytokinins (6-benzyladenine, kinetin, 2-isopentenyladenine and zeatin) tested.  Tracheary element formation is inhibited or delayed if the inductive medium is supplemented with an anticytokinin, antiauxin, or inhibitor of auxin transport.
authors.affiliation: Department of Biology, Yale University, New Haven, CT 06511.
authors.forename: D L
authors.initials: DL
authors.lastname: Church
authors.forename: A W
authors.initials: AW
authors.lastname: Galston
mesh_terms: D015084:2,4-Dichlorophenoxyacetic Acid / Q000032:analysis / Q000494:pharmacology; D000225:Adenine / Q000031:analogs & derivatives / Q000032:analysis / Q000494:pharmacology; D001593:Benzyl Compounds; D002454:Cell Differentiation / Q000187:drug effects* / Q000502:physiology; D002478:Cells, Cultured; D017077:Culture Media, Conditioned / Q000032:analysis / Q000494:pharmacology; D003583:Cytokinins / Q000032:analysis / Q000037:antagonists & inhibitors / Q000494:pharmacology; D007210:Indoleacetic Acids / Q000032:analysis / Q000037:antagonists & inhibitors / Q000494:pharmacology; D007541:Isopentenyladenosine; D007701:Kinetin / Q000032:analysis / Q000494:pharmacology; D009280:Naphthaleneacetic Acids / Q000032:analysis / Q000494:pharmacology; D059828:Plant Cells; D010937:Plant Growth Regulators / Q000037:antagonists & inhibitors* / Q000494:pharmacology*; D018515:Plant Leaves / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D018521:Plant Physiological Phenomena; D010944:Plants / Q000187:drug effects; D011687:Purines; D015026:Zeatin / Q000032:analysis / Q000494:pharmacology
chemical_list: D001593:Benzyl Compounds; D017077:Culture Media, Conditioned; D003583:Cytokinins; D007210:Indoleacetic Acids; D009280:Naphthaleneacetic Acids; D010937:Plant Growth Regulators; D011687:Purines; C001478:N(6)-(delta(2)-isopentenyl)adenine; D015084:2,4-Dichlorophenoxyacetic Acid; C034182:1-naphthaleneacetic acid; C030737:indoleacetic acid; D007541:Isopentenyladenosine; D015026:Zeatin; D000225:Adenine; C480551:benzylaminopurine; D007701:Kinetin