Sequence verification of oligonucleotides by electrospray quadrupole time-of flight mass spectrometry.
The combination of electrospray ionization (ESI) and quadrupole time-of-flight (Q-Tof) mass spectrometry presents a powerful tool to verify/determine the sequence of oligonucleotides. An ESI-Q-Tof instrument provides better sensitivity and much higher resolution compared with either ESI-triple quadrupole or ESI-ion trap devices. With high-resolution capability, the quadrupole time-of-flight instrument can provide an isotope pattern to support the charge state assignment. This will improve the reliability of the assignments of sequence-related w or a-Base series ions and lead to accurate determination of the oligonucleotide sequence.
Ni
J
J
Department of Biotransformation, Wyeth-Ayerst Research, Princeton, NJ 08852, USA. ni_jinsong@allergan.com
Chan
K
K
D004247:DNA / Q000032:analysis; D009841:Oligonucleotides / Q000737:chemistry*; D012313:RNA / Q000032:analysis; D017422:Sequence Analysis, DNA / Q000379:methods*; D017423:Sequence Analysis, RNA / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D009841:Oligonucleotides; D012313:RNA; D004247:DNA