title: Angiogenesis is an independent prognostic factor in malignant mesothelioma.
abstract: Angiogenesis is essential for tumour growth beyond 1 to 2 mm in diameter. The clinical relevance of angiogenesis, as assessed by microvessel density (MVD), is unclear in malignant mesothelioma (MM). Immunohistochemistry was performed on 104 archival, paraffin-embedded, surgically resected MM samples with an anti-CD34 monoclonal antibody, using the Streptavidin-biotin complex immunoperoxidase technique. 93 cases were suitable for microvessel quantification. MVD was obtained from 3 intratumoural hotspots, using a Chalkley eyepiece graticule at x 250 power. MVD was correlated with survival by Kaplan-Meier and log-rank analysis. A stepwise, multivariate Cox model was used to compare MVD with known prognostic factors and the EORTC and CALGB prognostic scoring systems. Overall median survival from the date of diagnosis was 5.0 months. Increasing MVD was a poor prognostic factor in univariate analysis (P = 0.02). Independent indicators of poor prognosis in multivariate analysis were non-epithelial cell type (P = 0.002), performance status > 0 (P = 0.003) and increasing MVD (P = 0.01). In multivariate Cox analysis, MVD contributed independently to the EORTC (P = 0.006), but not to the CALGB (P = 0.1), prognostic groups. Angiogenesis, as assessed by MVD, is a poor prognostic factor in MM, independent of other clinicopathological variables and the EORTC prognostic scoring system. Further work is required to assess the prognostic importance of angiogenic regulatory factors in this disease.
authors.affiliation: University Department of Medical Oncology, Leicester Royal Infirmary, Osborne Building, Leicester LE1 5WW, UK.
authors.forename: J G
authors.initials: JG
authors.lastname: Edwards
authors.forename: G
authors.initials: G
authors.lastname: Cox
authors.forename: A
authors.initials: A
authors.lastname: Andi
authors.forename: J L
authors.initials: JL
authors.lastname: Jones
authors.forename: R A
authors.initials: RA
authors.lastname: Walker
authors.forename: D A
authors.initials: DA
authors.lastname: Waller
authors.forename: K J
authors.initials: KJ
authors.lastname: O'Byrne
mesh_terms: D000367:Age Factors; D018952:Antigens, CD34 / Q000378:metabolism; D015331:Cohort Studies; D004730:Endothelium, Vascular / Q000378:metabolism; D005260:Female; D006454:Hemoglobins / Q000032:analysis; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008654:Mesothelioma / Q000098:blood supply* / Q000175:diagnosis / Q000401:mortality; D018301:Neoplasms, Mesothelial / Q000098:blood supply* / Q000175:diagnosis / Q000401:mortality; D009389:Neovascularization, Pathologic / Q000175:diagnosis* / Q000378:metabolism; D010976:Platelet Count; D011379:Prognosis; D012307:Risk Factors; D015996:Survival Rate
chemical_list: D018952:Antigens, CD34; D006454:Hemoglobins