title: Evaluation of a concurrent multicomponent collection system for the collection and storage of WBC-reduced RBC apheresis concentrates.
abstract: BACKGROUND
  Multicomponent apheresis procedures offer the possibility of collecting blood components that are standardized, as compared to those available with whole-blood donations. A new separator program for the concurrent collection of RBCs, platelets, and plasma (Amicus, Baxter Healthcare) was evaluated.
  
  
  METHODS
  Apheresis donors (n = 47) underwent concurrent collection of RBCs, platelets, and plasma by use of the single-needle procedure of the Amicus blood cell separator. A standardized RBC volume (100% Hct) of 200 mL was targeted with either 1 or 2 platelet concentrate units, depending on the donor's predonation characteristics. After collection, the RBC component was sterilely connected to an RBC collection set (Amicus) to allow for the addition of 100 mL of saline-adenine-glucose-mannitol preservative solution and WBC reduction at either ambient temperature or 4 degrees C. The RBC units were subsequently stored at 2 to 6 degrees C for 42 days, and the following in vitro measures were evaluated over the storage period: blood cell counts including Hct and total Hb, plasma Hb, potassium, pH, ATP, and 2,3 DPG.
  
  
  RESULTS
  Procedure time averaged 74 +/- 9 minutes, and no adverse events were reported. The absolute RBC volume collected averaged 198 +/- 11 mL with an average Hct value of 83 +/- 2 percent. After filtration, the Hb content averaged 58.2 +/- 2.4 g per unit and residual WBCs averaged 0.038 +/- 0.015 x 10(6) per unit. Day 42 results showed that all units had on average more than 70-percent ATP maintenance, and all of the units had less than 0.8 percent he-molysis. All units had pH values higher than 6.5 on Day 42.
  
  
  CONCLUSIONS
  The concurrent multicomponent collection system (Amicus) can reliably collect a standardized RBC unit of good quality. In vitro testing of the RBCs collected and stored for 42 days met the Council of Europe criteria for transfusion.
authors.affiliation: Institute for Transfusion Medicine, Essen University Clinics, Essen, Germany. rainer.moog@uni-essen.de
authors.forename: R
authors.initials: R
authors.lastname: Moog
authors.forename: V
authors.initials: V
authors.lastname: Franck
authors.forename: J A
authors.initials: JA
authors.lastname: Pierce
authors.forename: N
authors.initials: N
authors.lastname: Müller
mesh_terms: D000255:Adenosine Triphosphate / Q000097:blood; D001781:Blood Component Removal; D001782:Blood Donors; D001793:Blood Preservation / Q000592:standards*; D001800:Blood Specimen Collection / Q000592:standards*; D004911:Erythrocyte Volume; D004912:Erythrocytes / Q000502:physiology*; D005260:Female; D006400:Hematocrit; D006454:Hemoglobins / Q000032:analysis; D006461:Hemolysis; D006801:Humans; D007937:Leukapheresis; D007958:Leukocyte Count; D008297:Male; D013997:Time Factors
chemical_list: D006454:Hemoglobins; D000255:Adenosine Triphosphate