[Heat shock-induced changes in the respiration of the yeast Saccharomyces cerevisiae].
The incubation of Saccharomyces cerevisiae at elevated temperature (45 degrees C) stimulated the respiration of yeast cells and decreased their survival rate. The respiration-deficient mutant of this yeast was found to be more tolerant to the elevated temperature than the wild-type strain. At the same time, the cultivation of the wild-type strain in an ethanol-containing medium enhanced the respiration, catalase activity, and thermotolerance of yeast cells, as compared with their growth in a glucose-containing medium. It is suggested that the enhanced respiration of yeast cells at 45 degrees C leads to an intense accumulation of reactive oxygen species, which may be one of the reasons for the heat shock-induced cell death.
Rikhvanov
E G
EG
Siberian Institute of Physiology and Biochemistry of Plants, Siberian Division, Russian Academy of Sciences, Irkutsk, Russia. eugene@sifibir.irk.ru
Varakina
N N
NN
Rusaleva
T M
TM
Rachenko
E I
EI
Kiseleva
V A
VA
Voĭnikov
V K
VK
D002374:Catalase / Q000378:metabolism; D000431:Ethanol; D006358:Hot Temperature; D009154:Mutation; D010100:Oxygen / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000502:physiology*
D017382:Reactive Oxygen Species; D000431:Ethanol; D002374:Catalase; D010100:Oxygen