Direct MALDI-TOF MS analysis of oligonucleotides on solid support through a photolabile linker.
MALDI-TOF mass spectrometry was used to analyze oligonucleotides still anchored to long-chain alkylamine controlled-pore glass (LCAA-CPG) through a photolabile linker. This technique is useful to follow supported chemical reactions in real time and monitor by-products formation.
Meyer
A
A
Laboratoire de Chimie Organique Biomoléculaire de Synthèse, UMR 5625 CNRS-UM2, Université Montpellier II, Place E. Bataillon, 34095, Montpellier, France.
Spinelli
N
N
Brés
J C
JC
Dell'Aquila
C
C
Morvan
F
F
Lefebvre
I
I
Rayner
B
B
Imbach
J L
JL
Vasseur
J J
JJ
D003432:Cross-Linking Reagents / Q000737:chemistry*; D005898:Glass / Q000737:chemistry; D016376:Oligonucleotides, Antisense / Q000032:analysis* / Q000138:chemical synthesis / Q000737:chemistry; D010777:Photochemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D003432:Cross-Linking Reagents; D016376:Oligonucleotides, Antisense