Synthesis of beta-D-glucosyl- and beta-D-fucosyl-glucoses using beta-glycosidase from Thermus thermophilus.
The thermostable beta-glycosidase from Thermus thermophilus, cloned and overexpressed in Escherichia coli was used to catalyze the transfer of beta-D-glucosyl and beta-D-fucosyl from the corresponding p-nitrophenyl-beta-D-glycopyranosides to a hydroxyl group of glucose in the synthesis of beta-D-glucosyl-D-glucopyranoses and beta-D-fucosyl-D-glucopyranoses. The yields in disaccharides produced under conditions of non-initial velocity were very attractive and the formation of the beta(1-3) linked disaccharides was largely favored. The enzyme could constitute a valuable biocatalyst for the synthesis of disaccharides involving such structures as, for example, Bifidus factors.
Fourage
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Laboratoire de Biochimie, Unité de Recherche sur la Biocatalyse, CNRS 2230, Faculté des Sciences et des Techniques, Université de Nantes, France.
Colas
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B
D004187:Disaccharides / Q000096:biosynthesis*; D019075:Electrophoresis, Capillary; D006026:Glycoside Hydrolases / Q000378:metabolism*; D006031:Glycosylation; D009005:Monosaccharides / Q000378:metabolism / Q000494:pharmacology; D016963:Thermus thermophilus / Q000201:enzymology*; D005644:alpha-L-Fucosidase / Q000378:metabolism; D001617:beta-Glucosidase / Q000378:metabolism*
D004187:Disaccharides; D009005:Monosaccharides; D006026:Glycoside Hydrolases; D001617:beta-Glucosidase; D005644:alpha-L-Fucosidase