title: Stray Cu(II) may cause oxidative damage when coordinated to the -TESHHK- sequence derived from the C-terminal tail of histone H2A.
abstract: CH(3)CO-Thr-Glu-Ser-His-His-Lys-NH(2), a hexapeptide representing the 120-125 sequence of histone H2A, coordinates Cu(II) ions efficiently. Monomeric complexes are formed. In the major complex at physiological pH, CuH(-1)L, Cu(II) is coordinated equatorially through the imidazole nitrogen of the His-4 residue and the amide nitrogens of the Ser-3 and His-4 residues, and axially through the imidazole nitrogen of the His-5 residue. This complex reacts with H(2)O(2) and the resulting reactive oxygen intermediate efficiently oxidizes 2'-deoxyguanosine. The underlying mechanism involves the formation of Cu(III) and a metal-bound hydroxyl radical species.
authors.affiliation: University of Ioannina, Department of Chemistry, Ioannina 45110, Greece.
authors.forename: M
authors.initials: M
authors.lastname: Mylonas
authors.forename: G
authors.initials: G
authors.lastname: Malandrinos
authors.forename: J
authors.initials: J
authors.lastname: Plakatouras
authors.forename: N
authors.initials: N
authors.lastname: Hadjiliadis
authors.forename: K S
authors.initials: KS
authors.lastname: Kasprzak
authors.forename: A
authors.initials: A
authors.lastname: Krezel
authors.forename: W
authors.initials: W
authors.lastname: Bal
mesh_terms: D003300:Copper / Q000737:chemistry*; D005609:Free Radicals; D006657:Histones / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry; D009842:Oligopeptides / Q000737:chemistry*; D016877:Oxidants / Q000737:chemistry; D010084:Oxidation-Reduction; D018384:Oxidative Stress
chemical_list: D005609:Free Radicals; D006657:Histones; D009842:Oligopeptides; D016877:Oxidants; D003300:Copper; D006861:Hydrogen Peroxide