Photosynthesis and respiration of a wheat stand at reduced atmospheric pressure and reduced oxygen.
A 34-day functional test was conducted in Johnson Space Center's Variable Pressure Growth Chamber (VPGC) to determine responses of a wheat stand to reduced pressure (70 kPa) and modified partial pressures of carbon dioxide and oxygen. Reduced pressure episodes were generally six to seven hours in duration, were conducted at reduced ppO2 (14.7 kPa), and were interrupted with longer durations of ambient pressure (101 kPa). Daily measurements of stand net photosynthesis (Pn) and dark respiration (DR) were made at both pressures using a ppCO2 of 121 Pa. Corrections derived from leakage tests were applied to reduced pressure measurements. Rates of Pn at reduced pressure averaged over the complete test were 14.6% higher than at ambient pressure, but rates of DR were unaffected. Further reductions in ppO2 were achieved with a molecular sieve and were used to determine if Pn was enhanced by lowered O2 or by lowered pressure. Decreased ppO2 resulted in enhanced rates of Pn, regardless of pressure, but the actual response was dependent on the ratio of ppO2/ppCO2. Over the range of ppO2/ppCO2 of 80 to 200, the rate of Pn declined linearly. Rate of DR was unaffected over the same range and by dissolved O2 levels down to 3.1 ppm, suggesting that normal rhizosphere and canopy respiration occur at atmospheric ppO2 levels as low as 11 kPa. Partial separation of effects attributable to oxygen and those related to reduced pressure (e.g. enhanced diffusion of CO2) was achieved from analysis of a CO2 drawdown experiment. Results will be used for design and implementation of studies involving complete crop growth tests at reduced pressure.
Corey
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KA
Department of Plant & Soil Science, University of Massachusetts, Amherst 01003, USA.
Barta
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DJ
Henninger
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DL
D001274:Atmospheric Pressure; D018533:Biomass; D002245:Carbon Dioxide / Q000378:metabolism*; D019069:Cell Respiration; D003624:Darkness; D004780:Environment, Controlled; D006801:Humans; D008021:Life Support Systems; D010100:Oxygen / Q000378:metabolism*; D010313:Partial Pressure; D010788:Photosynthesis / Q000502:physiology*; D018526:Plant Transpiration; D014908:Triticum / Q000254:growth & development / Q000378:metabolism*
D002245:Carbon Dioxide; D010100:Oxygen