[Insulin degradation in hepatocytes and erythrocytes of rats in normal condition and in experimental diabetes].
Insulin metabolism in erythrocytes and hepatocytes of normal and experimental diabetic rats was studied. Insulin-degrading complex of erythrocytes and hepatocytes was investigated at low insulin concentration in blood. Kinetic parameters of insulinase depend on insulin concentration in blood have shown. Thermo-acid resistant non-competitive endogenous inhibitors were purified from erythrocytes and hepatocytes. Increase of endogenous inhibitors influence insulin-degrading enzyme in diabetes. Synthesis of insulin-degrading enzyme in hepatocytes of diabetic rats was reduced. These results suggest that insulin-degrading activity in erythrocytes and hepatocytes is regulated by endogenous inhibitors, by synthesis of insulinase. This change of blood insulin concentration influences practically all the components of insulin-degrading complex.
Nikolaev
S L
SL
Department of Biochemistry, Saint-Petersburg State Chemical Pharmaceutical Academy, 197376 Saint-Petersburg.
Strelkova
M A
MA
Komov
V P
VP
D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism*; D006868:Hydrolysis; D007328:Insulin / Q000378:metabolism*; D007339:Insulysin / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D008297:Male; D051381:Rats
D007328:Insulin; D007339:Insulysin