title: Signal transduction pathways involved in low intensity He-Ne laser-induced respiratory burst in bovine neutrophils: a potential mechanism of low intensity laser biostimulation.
abstract: OBJECTIVE
  Low intensity He-Ne laser irradiation has been reported to induce respiratory burst of neutrophils for a long time, but the mechanism remains obscure. We speculated that it is mediated by some signal transduction pathways.
  
  
  METHODS
  The protein tyrosine kinases (PTKs) inhibitor, genistein, the phospholipase C (PLC) inhibitor, U-73122, and the protein kinase C (PKC) inhibitor, calphostin C, were used to probe signal transduction pathways of respiratory burst of bovine neutrophils which were induced by He-Ne laser at a dose of 300 J/m(2), respectively.
  
  
  RESULTS
  The inhibitor of PTKs can completely inhibit the He-Ne laser-induced respiratory burst of neutrophils. PLC and PKC inhibitors can obviously reduce it, but not fully inhibit it.
  
  
  CONCLUSIONS
  These results suggest that PTKs play a key role in the He-Ne laser-induced respiratory burst of neutrophils and [PTK-PLC-PKC-NADPH oxidase] signal transduction pathways may be involved in this process.
authors.affiliation: Laboratory of Light Transmission Optics, South China Normal University, Guangzhou, GD 510631, China.
authors.forename: R
authors.initials: R
authors.lastname: Duan
authors.forename: T C
authors.initials: TC
authors.lastname: Liu
authors.forename: Y
authors.initials: Y
authors.lastname: Li
authors.forename: H
authors.initials: H
authors.lastname: Guo
authors.forename: L B
authors.initials: LB
authors.lastname: Yao
mesh_terms: D000818:Animals; D002417:Cattle; D007834:Lasers; D009504:Neutrophils / Q000378:metabolism*; D016897:Respiratory Burst; D015398:Signal Transduction