title: Depletion of praziquantel in plasma and muscle tissue of cultured rockfish Sebastes schlegeli after oral and bath treatment.
abstract: Depletion of praziquantel in plasma and muscle tissue after oral and bath treatments was studied in cultured rockfish Sebastes schlegeli. In the oral treatment, a single dose of 400 mg praziquantel kg(-1) body weight was administered by intubation of the stomach. A bath treatment at 100 ppm of praziquantel for 4 min was also carried out. Plasma and muscle tissue samples were collected at 3, 6, 12, 24, 48, 72, 96, 120, 144 and 168 h post-treatment, and analyzed for praziquantel by reversed-phase HPLC using diazepam as the internal standard. Following oral treatment, praziquantel was detected in plasma and muscle tissue until 96 h after treatment. In plasma the praziquantel concentration was highest at the 9 h sampling time and declined sharply at the 48 h sampling point. The concentrations of praziquantel in the muscle tissue were lower than those in the plasma, and the highest value was found at the 9 h sampling time. Following bath treatment, praziquantel was found in plasma and muscle tissue until 72 and 24 h after treatment, respectively. In plasma the praziquantel concentration was highest at the 12 h sampling time and declined sharply thereafter. The concentrations of praziquantel in the muscle tissue were significantly lower than those in the plasma, and the concentrations declined consistently with time.
authors.affiliation: Department of Aquatic Life Medicine, College of Fisheries Science, Pukyong National University, Pusan, Korea. khkim@pknu.ac.kr
authors.forename: K H
authors.initials: KH
authors.lastname: Kim
authors.forename: C S
authors.initials: CS
authors.lastname: Kim
authors.forename: J W
authors.initials: JW
authors.lastname: Kim
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000871:Anthelmintics / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics*; D017756:Aquaculture; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D004350:Drug Residues / Q000032:analysis / Q000493:pharmacokinetics*; D005393:Fish Diseases / Q000188:drug therapy; D005399:Fishes / Q000097:blood / Q000378:metabolism*; D009132:Muscles / Q000378:metabolism*; D010273:Parasitic Diseases, Animal / Q000188:drug therapy; D011223:Praziquantel / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics*; D014018:Tissue Distribution
chemical_list: D000871:Anthelmintics; D011223:Praziquantel