title: Induction of chromosome aberrations in mammalian cells after heavy ion exposure.
abstract: The induction of chromosome aberrations by heavy charged particles was studied in V79 Chinese hamster cells over a wide range of energies (3-100 MeV/u) and LET (20-16000 keV/micrometer).  For comparison, X-ray experiments were performed.  Our data indicate quantitative and qualitative differences in the response of cells to particle and x-ray irradiation.  For the same level of cell survival the amount of damaged cells which can be observed is smaller in heavy ion (11.4 MeV/u Ar) irradiated samples.  The highest yield of damaged cells is found 8 to 12 hours after particle irradiation and 4 hours after x-irradiation.  Differences in the amount of damaged cells are attributed to cell cycle perturbations which interfere with the expression of damage.  After heavy ion exposure the amount of cells reaching mitosis (mitotic index) decreases drastically and not all damaged cells reach mitosis within 48 hours after exposure.  A portion of cells die in interphase.  Cell cycle delays induced by x-ray irradiation are less pronounced and all cells reach the first post-irradiation mitosis within 24 hours after irradiation.  Additionally, the damage produced by charged particles seems to be more severe.  The disintegration of chromosomes was only observed after high LET radiation:  an indication of the high and local energy deposition in the particle track.  Only cross sections for the induction of chromosome aberrations in mitotic cells were reported in this paper because of the problems arising from the drastic cell cycle perturbations.  In this case, cells were irradiated in mitosis and assayed immediately.
authors.affiliation: Gesellschaft für Schwerionenforschung, Biophysik, Darmstadt, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Ritter
authors.forename: W
authors.initials: W
authors.lastname: Kraft-Weyrather
authors.forename: M
authors.initials: M
authors.lastname: Scholz
authors.forename: G
authors.initials: G
authors.lastname: Kraft
mesh_terms: D000818:Animals; D001128:Argon; D002453:Cell Cycle / Q000528:radiation effects; D002460:Cell Line; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000528:radiation effects; D003359:Cosmic Radiation / Q000009:adverse effects*; D006224:Cricetinae; D004307:Dose-Response Relationship, Radiation; D004601:Elementary Particles; D007477:Ions; D018499:Linear Energy Transfer; D008938:Mitosis / Q000528:radiation effects; D009154:Mutation; D010315:Particle Accelerators; D011853:Radiobiology; D014965:X-Rays
chemical_list: D007477:Ions; D001128:Argon