Protein engineering by cell-surface display.
Protein libraries displayed on cell surfaces can be labeled with soluble ligands exhibiting well-characterized binding equilibria and dissociation kinetics, and then quantitatively screened by flow cytometry at a rate of >10(4) clones/second. The promise of cell-surface display for directed evolution is being realized, with significant improvements recently reported in protein ligand binding affinity, stability, expression and enzymatic activity.
Wittrup
K D
KD
Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. wittrup@mit.edu
D001665:Binding Sites / Q000502:physiology; D019020:Directed Molecular Evolution / Q000379:methods*; D004789:Enzyme Activation / Q000502:physiology; D004795:Enzyme Stability; D005434:Flow Cytometry; D007700:Kinetics; D008024:Ligands; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism*; D019151:Peptide Library; D015202:Protein Engineering / Q000379:methods*; D013812:Therapeutics
D008024:Ligands; D008565:Membrane Proteins; D019151:Peptide Library