Solution solution: using NMR models for molecular replacement.
NMR structures can serve as a good source of search models in crystal structure determination by molecular replacement. However, owing to the inherent problems of NMR models, this procedure is not always straightforward. Here, an updated overview is presented with particular emphasis on the preparation of NMR search models and the latest trends in methodology. An experimental protocol developed recently is described and results on its use in solving a new structure as well as its test against a difficult published case are presented.
Chen
Y W
YW
Cambridge University Chemical Laboratory and Centre for Protein Engineering, Hills Road, Cambridge CB2 2QH, England. ywc@mrc-lmb.cam.ac.uk
D018360:Crystallography, X-Ray; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D010940:Plant Proteins / Q000737:chemistry; D012269:Ribosomal Proteins / Q000737:chemistry; D012984:Software
C114195:CHF1 protein, Zea mays; D010940:Plant Proteins; D012269:Ribosomal Proteins; C035201:ribosomal protein L30