title: Molecular cloning and sequence analysis of alpha- and beta-globin cDNAs from yellowtail, Seriola quinqueradiata.
abstract: Two alpha- and two beta-globin genes from the yellowtail were cloned and their nucleotide sequences determined. The entire open reading frames of alpha-globin A and B genes were 432 and 435 bp long, respectively, whereas the corresponding values of the yellowtail beta-globin A and B genes were both 447 bp. Amino acid identity of the yellowtail alpha- or beta-globin gene compared with those reported in other vertebrates including shark, teleosts and human, ranged from 35.4 to 83.0%. The yellowtail alpha-globin B has a unique inserted amino acid residue in the 48th position, not found in other fish alpha-globin genes. The orientation of alpha- and beta-globin genes open reading frames was head-to-head.
authors.affiliation: Faculty of Agriculture, Miyazaki University, Miyazaki 889-2192, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Okamoto
authors.forename: M
authors.initials: M
authors.lastname: Sakai
authors.forename: M
authors.initials: M
authors.lastname: Miyata
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D005399:Fishes / Q000235:genetics*; D015723:Gene Library; D008040:Genetic Linkage; D005914:Globins / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D005914:Globins