trans-10,cis-12-Conjugated linoleic acid reduces leptin secretion from 3T3-L1 adipocytes.
The trans10,cis12 (t10c12) isomer of conjugated linoleic acid (CLA) has been shown to inhibit heparin-releasable lipoprotein lipase activity, reduce lipid stores in cultured 3T3-L1 adipocytes, and, when fed to mice, reduce body fat gain. We now report that t10c12 CLA significantly reduced leptin secretion from cultured 3T3-L1 adipocytes, and reduced leptin mRNA levels within the cells. Similar effects were produced by conjugated nonadecadienoic acid (a 19-carbon CLA cognate that is more effective than CLA in reducing body fat gain in mice), the lipoxygenase inhibitor nordihydroguaiaretic acid (which is synergistic with CLA in reducing body fat gain in mice), and ciglitazone (TZD, a PPARgamma agonist). Feeding mice diet supplemented with 0.5% t10c12 CLA for 4 weeks significantly reduced body fat gain, serum leptin levels and adipocyte leptin mRNA expression, without affecting feed intake or body weight. These data provide new insights into apparent mechanistic similarities among t10c12 CLA, CNA, NDGA, and TZD.
Kang
K
K
Food Research Institute, Department of Food Microbiology and Toxicology, University of Wisconsin-Madison, 1925 Willow Drive, Madison, Wisconsin 53706, USA.
Pariza
M W
MW
D016475:3T3 Cells; D017667:Adipocytes / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D007004:Hypoglycemic Agents / Q000494:pharmacology; D020738:Leptin / Q000378:metabolism*; D019787:Linoleic Acid / Q000737:chemistry / Q000494:pharmacology*; D008297:Male; D009637:Masoprocol / Q000494:pharmacology; D051379:Mice; D008813:Mice, Inbred ICR; D013844:Thiazoles / Q000494:pharmacology; D045162:Thiazolidinediones
D000975:Antioxidants; D007004:Hypoglycemic Agents; D020738:Leptin; D013844:Thiazoles; D045162:Thiazolidinediones; D009637:Masoprocol; D019787:Linoleic Acid; C039671:ciglitazone