title: Enzymic synthesis of indole-3-acetyl-1-O-beta-d-glucose.  II.  Metabolic characteristics of the enzyme.
abstract: The synthesis of indole-3-acetyl-1-O-beta-D-glucose from indole-3-acetic acid (IAA) and uridine diphosphoglucose (UDPG) has been shown to be a reversible reaction with the equilibrium away from ester formation and toward formation of IAA.  The enzyme occurs primarily in the liquid endosperm of the corn kernel but some activity occurs in the embryo.  It is relatively specific showing no glucose ester formation with oxindole-3-acetic acid or 7-hydroxy-oxindole-3-acetic acid, and low activity with phenylpropene acids, such as rho-coumaric acid.  The enzyme is also specific for the nucleotide sugar showing no activity with UDPGalactose or UDPXylose.  The enzyme is inhibited by inorganic pyrophosphate, by phosphate esters and by phospholipids, particularly phosphatidyl ethanolamine.  The enzyme is inhibited by zeatin, by 2,4-dichlorophenoxy-acetic acid, by IAA-myo-inositol and IAA-glucan, but not by zeatin riboside, and only weakly by gibberellic acid, abscisic acid and kinetin.  The reaction is slightly stimulated by both calcium and calmodulin and, in some cases, by thiol compounds.  The role of this enzyme in the homeostatic control of indole-3-acetic acid levels in Zea mays is discussed.
authors.affiliation: Department of Botany and Plant Pathology, Michigan State University, East Lansing 48824-1312, USA.
authors.forename: A J
authors.initials: AJ
authors.lastname: Leznicki
authors.forename: R S
authors.initials: RS
authors.lastname: Bandurski
mesh_terms: D015084:2,4-Dichlorophenoxyacetic Acid / Q000494:pharmacology; D002845:Chromatography / Q000379:methods; D011756:Diphosphates / Q000494:pharmacology; D005936:Glucans / Q000494:pharmacology; D005964:Glucosyltransferases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D007210:Indoleacetic Acids / Q000378:metabolism*; D010743:Phospholipids / Q000494:pharmacology; D010937:Plant Growth Regulators / Q000378:metabolism* / Q000494:pharmacology; D013379:Substrate Specificity; D014530:Uridine Diphosphate / Q000378:metabolism; D014532:Uridine Diphosphate Glucose / Q000378:metabolism*; D003313:Zea mays / Q000201:enzymology* / Q000378:metabolism; D015026:Zeatin / Q000494:pharmacology
chemical_list: D011756:Diphosphates; D005936:Glucans; D007210:Indoleacetic Acids; D010743:Phospholipids; D010937:Plant Growth Regulators; C037009:1-O-indol-3-ylacetylglucose; D015084:2,4-Dichlorophenoxyacetic Acid; D014530:Uridine Diphosphate; C030737:indoleacetic acid; D015026:Zeatin; D005964:Glucosyltransferases; C071643:indole-3-acetate beta-glucosyltransferase; D014532:Uridine Diphosphate Glucose