Influence of clinorotation and fettering stress on tail regeneration of Triturus vulgaris (Urodela).
Tail-amputated adult Triturus vulgaris, fettered in cuvettes of a fast-rotating clinostat were exposed to simulated weightlessness (60 rpm; equiv. to 10(-3)-10(-4) g), during a 14-day period. To feed and clean the animals rotation was stopped once a day for approx. 10 min. To test the influence of the fettering stress, a second series of animals was kept separately under normal earth conditions without rotation. A further control series was kept in a dark container without any handicap. While tail regeneration of the rotated animals was markedly accelerated, the fettered-only animals showed a considerably less marked acceleration effect. At the end of the 14-day period, all regenerates were reamputated together with an additional 5 mm of the tail stump. Although this second level of amputation was distant from the first, the regenerative growth rate of the rotated series was accelerated 123% in contrast to both the control and the fettered-only series. Our results demonstrate that the growth acceleration is induced by clinorotation. Fettering stress has no comparable influence. The growth promoting effect is not limited to the regenerating area.
Anton
H J
HJ
Zoological Institute, University of Cologne, Germany.
Grigoryan
E N
EN
Krupp-Beyerlein
K
K
Pitzer
H
H
Mitashov
V I
VI
D000671:Amputation, Surgical; D000818:Animals; D006112:Gravitation; D007103:Immobilization / Q000502:physiology*; D012038:Regeneration; D012399:Rotation; D013312:Stress, Physiological; D013623:Tail / Q000502:physiology*; D014319:Triturus; D018474:Weightlessness Simulation