title: Effects of microgravity on the binding of acetylsalicylic acid by Rhizobium leguminosarum bv. trifolii.
abstract: Bacteroids can be induced in vitro by treating growing Rhizobium leguminosarum bv. trifolii with succinic acid or succinic acid structural analogs like acetysalicylic acid. Quantitating bacteroid induction by measuring acetylsalicylic binding under normal (1 g) conditions showed two forms of binding to occur. In one form of binding cells immediately bound comparatively high levels of acetylsalicylic acid, but the binding was quickly reversed. The second form of binding increased with time by first order kinetics and reached saturation in 40 s. Similar experiments performed in the microgravity environment aboard the NASA 930 aircraft showed only one form of binding and total acetylsalicyclic acid bound was 32% higher than at 1 g.
authors.affiliation: BioServe Space Technologies, Kansas State University, Manhattan 66506-4901, USA.
authors.forename: J E
authors.initials: JE
authors.lastname: Urban
authors.forename: R
authors.initials: R
authors.lastname: Gerren
authors.forename: J
authors.initials: J
authors.lastname: Zoelle
mesh_terms: D001241:Aspirin / Q000378:metabolism* / Q000493:pharmacokinetics; D007887:Fabaceae; D006112:Gravitation; D009586:Nitrogen Fixation; D010946:Plants, Medicinal; D011485:Protein Binding; D016961:Rhizobium leguminosarum / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D013026:Space Flight; D013559:Symbiosis; D014893:Weightlessness
chemical_list: D001241:Aspirin