title: A new type of muconate cycloisomerase from Rhodococcus rhodochrous strain 89.
abstract: Muconate cycloisomerase (MCI) was purified from Rhodococcus rhodochrous 89 grown on phenol. The enzyme appears to contain two different type subunits with molecular masses 35.5 and 37 kD. The N-terminal amino acid sequence of both subunits showed more similarity to corresponding enzymes from gram-negative bacteria than to one from Rhodococcus opacus 1CP. MCI from R. rhodochrous 89, like analogous enzymes from gram-negative bacteria, can convert 2-chloromuconate (2-CM) with the formation of both, 2- and 5-chloromuconolactones (CML) as intermediates. Nevertheless, its unique ability to convert 5-CML to cis- but not to trans-dienelactone sets it apart from all known chloromuconate cycloisomerases from gram-negative and gram-positive bacteria.
authors.affiliation: Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia.
authors.forename: I P
authors.initials: IP
authors.lastname: Solyanikova
authors.forename: M
authors.initials: M
authors.lastname: Schlömann
authors.forename: L A
authors.initials: LA
authors.lastname: Golovleva
mesh_terms: D000272:Adipates / Q000378:metabolism*; D000595:Amino Acid Sequence; D019753:Intramolecular Lyases / Q000302:isolation & purification* / Q000378:metabolism*; D008969:Molecular Sequence Data; D021122:Protein Subunits; D012240:Rhodococcus / Q000201:enzymology*; D013011:Sorbic Acid / Q000031:analogs & derivatives* / Q000378:metabolism*
chemical_list: D000272:Adipates; D021122:Protein Subunits; C088140:2-chloromuconic acid; D019753:Intramolecular Lyases; C021811:muconate cycloisomerase; D013011:Sorbic Acid