Synthesis of chimeric oligonucleotides containing internucleosidic phosphodiester and S-pivaloylthioethyl phosphotriester residues.
Novel oligonucleotide analogs that bear phosphodiester and bioreversible S-pivaloyl 2-mercaptoethyl (SPME) phosphate triester internucleosidic linkages are described. Their synthesis employs a novel methodology of oligonucleotide deprotection under mild, non-aqueous conditions.
Guzaev
A P
AP
Department of Medicinal Chemistry, Isis Pharmaceuticals, 2292 Faraday Ave., Carlsbad, California 92008, USA.
Bhat
B
B
Balow
G
G
Manoharan
M
M
D016376:Oligonucleotides, Antisense / Q000138:chemical synthesis*; D010755:Organophosphates / Q000138:chemical synthesis* / Q000737:chemistry; D009943:Organophosphorus Compounds / Q000138:chemical synthesis / Q000737:chemistry; D009946:Organothiophosphorus Compounds / Q000737:chemistry; D010421:Pentanoic Acids / Q000737:chemistry
D016376:Oligonucleotides, Antisense; D010755:Organophosphates; D009943:Organophosphorus Compounds; D009946:Organothiophosphorus Compounds; D010421:Pentanoic Acids; C434331:phosphoramidite