The use and evaluation of 2 + 2 photoaddition in immobilization of oligonucleotides on a three dimensional hydrogel matrix.
Photochemical attachment of synthetic oligonucleotides on the three dimensional surface of a polyacrylamide based hydrogel was used in the specific detection of target oligonucleotides. Covalent attachment of the oligonucleotide to the hydrogel was mediated by the incorporation of a 2 + 2 photo-attachable functional group in both the hydrogel and the oligonucleotide probe. Expression and SNP assays were used to evaluate this platform.
Elghanian
R
R
Motorola BioChip Systems, Northbrook, Illinois, USA.
Xu
Y
Y
McGowen
J
J
Seithoff
M
M
Liu
C G
CG
Winick
J
J
Fuller
N
N
Ramakrishnan
R
R
Beuhler
A
A
Johnson
T
T
Mazumder
A
A
Brush
C K
CK
D000180:Acrylic Resins / Q000737:chemistry; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D020411:Oligonucleotide Array Sequence Analysis; D015345:Oligonucleotide Probes / Q000737:chemistry; D009841:Oligonucleotides / Q000737:chemistry*; D010777:Photochemistry; D000099166:Polyacrylamides
D000180:Acrylic Resins; D020100:Hydrogels; D015345:Oligonucleotide Probes; D009841:Oligonucleotides; D000099166:Polyacrylamides