title: Is profound hypothermia required for storage of cardiac allografts?
abstract: BACKGROUND
  Improved methods of cardiac allograft protection are required to expand the pool of potentially available organs and to enhance the recovery of grafts subjected to prolonged ischemia. We have previously demonstrated that limited coronary perfusion provided by donor blood harvested at the time of organ procurement can improve both metabolic and functional recovery after transplantation. In this study we evaluated the hypothesis that limited coronary perfusion may enable prolonged cardiac storage while avoiding the potentially detrimental effects of profound hypothermia.
  
  
  METHODS
  Fourteen orthotopic cardiac transplants were performed in female Yorkshire pigs by using donor blood perfusion during 5 hours of either tepid (25 degrees C) or cold (4 degrees C) storage. Assessments of myocardial metabolism and function were performed at baseline and after 45 minutes of normothermic (37 degrees C) reperfusion.
  
  
  RESULTS
  Hearts protected with tepid perfusion displayed improved recovery of myocardial function (89% +/- 18% vs 63% +/- 25%, P =.05). Diastolic compliance was adversely affected in both groups after transplantation. Aerobic myocardial metabolism was better preserved in the tepid group.
  
  
  CONCLUSIONS
  Profound hypothermia results in depressed myocardial metabolic and functional recovery after transplantation. Limited coronary perfusion with shed donor blood can permit cardiac allograft storage at tepid temperatures, resulting in improved myocardial performance.
authors.affiliation: Heart Transplant Program, University of Toronto, Ontario, Canada.
authors.forename: V
authors.initials: V
authors.lastname: Rao
authors.forename: C M
authors.initials: CM
authors.lastname: Feindel
authors.forename: G
authors.initials: G
authors.lastname: Cohen
authors.forename: M A
authors.initials: MA
authors.lastname: Borger
authors.forename: P
authors.initials: P
authors.lastname: Boylen
authors.forename: H J
authors.initials: HJ
authors.lastname: Ross
mesh_terms: D000818:Animals; D001804:Blood Transfusion, Autologous / Q000379:methods*; D003187:Compliance; D003326:Coronary Circulation; D015925:Cryopreservation / Q000379:methods*; D003971:Diastole; D004195:Disease Models, Animal; D005260:Female; D006085:Graft Survival; D016027:Heart Transplantation / Q000009:adverse effects / Q000379:methods; D007036:Hypothermia, Induced / Q000009:adverse effects / Q000379:methods*; D009200:Myocardial Contraction / Q000502:physiology*; D009206:Myocardium / Q000378:metabolism*; D010101:Oxygen Consumption; D020127:Recovery of Function; D015427:Reperfusion Injury / Q000209:etiology* / Q000378:metabolism / Q000503:physiopathology / Q000517:prevention & control*; D013552:Swine; D013696:Temperature; D014184:Transplantation, Homologous; D016896:Treatment Outcome; D016277:Ventricular Function, Left