title: Effect of track structure and radioprotectors on the induction of oncogenic transformation in murine fibroblasts by heavy ions.
abstract: The oncogenic potential of high-energy 56Fe particles (1 GeV/nucleon) accelerated with the Alternating Gradient Synchrotron at the Brookhaven National Laboratory was examined utilizing the mouse C3H 10T1/2 cell model. The dose-averaged LET for high-energy 56Fe is estimated to be 143 keV/micrometer with the exposure conditions used in this study. For 56Fe ions, the maximum relative biological effectiveness (RBEmax) values for cell survival and oncogenic transformation were 7.71 and 16.5 respectively. Compared to 150 keV/micrometer 4He nuclei, high-energy 56Fe nuclei were significantly less effective in cell killing and oncogenic induction. The prostaglandin E1 analog misoprostol, an effective oncoprotector of C3H 10T1/2 cells exposed to X rays, was evaluated for its potential as a radioprotector of oncogenic transformation with high-energy 56Fe. Exposure of cells to misoprostol did not alter 56Fe cytotoxicity or the rate of 56Fe-induced oncogenic transformation.
authors.affiliation: Center for Radiological Research, Columbia University, New York 10032, USA.
authors.forename: R C
authors.initials: RC
authors.lastname: Miller
authors.forename: S G
authors.initials: SG
authors.lastname: Martin
authors.forename: W R
authors.initials: WR
authors.lastname: Hanson
authors.forename: S A
authors.initials: SA
authors.lastname: Marino
authors.forename: E J
authors.initials: EJ
authors.lastname: Hall
mesh_terms: D000818:Animals; D002460:Cell Line; D002470:Cell Survival; D002471:Cell Transformation, Neoplastic; D004307:Dose-Response Relationship, Radiation; D004341:Drug Evaluation; D005347:Fibroblasts / Q000166:cytology / Q000528:radiation effects; D020450:Heavy Ions; D006371:Helium; D007501:Iron; D018499:Linear Energy Transfer; D051379:Mice; D008809:Mice, Inbred C3H; D016595:Misoprostol / Q000494:pharmacology; D011829:Radiation Dosage; D011837:Radiation-Protective Agents / Q000494:pharmacology; D012062:Relative Biological Effectiveness; D017356:Synchrotrons
chemical_list: D011837:Radiation-Protective Agents; D016595:Misoprostol; D006371:Helium; D007501:Iron