title: Eucalyptus camaldulensis: volatiles from immature flowers and high production of 1,8-cineole and beta-pinene by in vitro cultures.
abstract: Calli of Eucalyptus camaldulensis Dehn were induced, for the first time, from immature flowers and stamens and established in the presence of 2,4-D and BA, under dark and light conditions. Immature flowers, of the same type used for callus induction, were submitted to hydrodistillation while the induced calli were extracted with n-pentane. The constituents of the n-pentane extracts and of the hydrodistillate were identified by GC-MS. The main constituents of the hydrodistillate from immature flowers were 1,8-cineole (34.7%), beta-pinene (7.7%), and spathulenol (9.5%). The n-pentane extract from calli developed from stamens consisted only of alkanes, alkenes and alcohols, while that of calli developed from immature flowers consisted mainly of monoterpenes (92.08-96.56%). The main monoterpenes produced in these calli, cultured in darkness and under light conditions, were 1,8-cineole, 62.70 and 69.26% as well as beta-pinene, 27.09 and the 25.31%, respectively.
authors.affiliation: Division of Botany, Department of Biology, University of Athens, 157 71, Athens, Greece.
authors.forename: A
authors.initials: A
authors.lastname: Giamakis
authors.forename: O
authors.initials: O
authors.lastname: Kretsi
authors.forename: I
authors.initials: I
authors.lastname: Chinou
authors.forename: C G
authors.initials: CG
authors.lastname: Spyropoulos
mesh_terms: D000081008:Bicyclic Monoterpenes; D001643:Bridged Bicyclo Compounds / Q000378:metabolism*; D003511:Cyclohexanols / Q000378:metabolism*; D000077591:Eucalyptol; D005052:Eucalyptus / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D066298:In Vitro Techniques; D039821:Monoterpenes; D009822:Oils, Volatile / Q000737:chemistry; D013729:Terpenes / Q000378:metabolism*; D014835:Volatilization
chemical_list: D000081008:Bicyclic Monoterpenes; D001643:Bridged Bicyclo Compounds; D003511:Cyclohexanols; D039821:Monoterpenes; D009822:Oils, Volatile; D013729:Terpenes; C010789:beta-pinene; D000077591:Eucalyptol