title: Electroinduced extraction of beta-galactosidase from Kluyveromyces lactis.
abstract: A new methodology for the extraction of beta-galactosidase from the yeast Kluyveromyces lactis was obtained by electropulsation. The application of a series of electric pulses (2 ms duration, 1 Hz frequency, and 4-4.5 kV/cm field strength) to fresh cells suspended in deionized water, followed by incubation in PBS, led to a spontaneous slow release of enzyme at a yield of 75-80% without any further treatment. Most of the enzyme was extracted within 8 h after electropulsation. This release was dependent on the growth phase. The specific activity of beta-galactosidase in the supernatant of pulsed cells was higher by a factor of 1.5-1.7 in comparison with crude extract.
authors.affiliation: Faculty of Biology, University of Sofia, Bulgaria.
authors.forename: V
authors.initials: V
authors.lastname: Ganeva
authors.forename: B
authors.initials: B
authors.lastname: Galutzov
authors.forename: N
authors.initials: N
authors.lastname: Eynard
authors.forename: J
authors.initials: J
authors.lastname: Teissié
mesh_terms: D001709:Biotechnology / Q000379:methods; D004574:Electromagnetic Fields; D004790:Enzyme Induction; D007716:Kluyveromyces / Q000201:enzymology* / Q000254:growth & development; D001616:beta-Galactosidase / Q000096:biosynthesis* / Q000302:isolation & purification*
chemical_list: D001616:beta-Galactosidase