Immunological analysis of agglutination in Dirofilaria immitis microfllariae.
The mechanism of agglutination phenomenon of Dirofilaria immitis microfilariae was analyzed. Circulating microfilariae were collected from a D. immitis-infected microfilaremic dog and cultured in the several kinds of sera from dogs and animals. The agglutination of D. immitis microfilariae is a specific phenomenon due to some immune complexes formed with the anti-microfilarial antibody, heat-instable factor(s) and excretory-secretory products of microfilariae. Only live microfilariae were agglutinated and the agglutinated microfilariae remained alive as long as 27 days in culture in vitro.
Hayasaki
M
M
Veterinary Clinical Center, School of Veterinary Medicine, Yamaguchi University, Japan.
D000371:Agglutination; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D006224:Cricetinae; D004183:Dirofilaria immitis / Q000276:immunology* / Q000502:physiology; D004184:Dirofilariasis / Q000276:immunology / Q000469:parasitology*; D004283:Dog Diseases / Q000276:immunology / Q000469:parasitology*; D004285:Dogs; D019084:Fluorescent Antibody Technique, Indirect; D051379:Mice; D008807:Mice, Inbred BALB C; D008842:Microfilariae / Q000276:immunology / Q000469:parasitology; D011817:Rabbits
D000911:Antibodies, Monoclonal