Messenger RNA isolation using novel PNA analogues.
Homo-Thy hetero-oligomer probes composed of trans-4-hydroxy-L-proline based PNA-like (HypNA) monomers and phosphono PNA (pPNA) monomers demonstrated strong binding to complementary poly A+ RNA strands. We used a mixture of chimeric oligomers containing both "linear" and "clamping" PNA-analogues to develop an mRNA isolation procedure and demonstrate the improved recovery of RNA molecules with secondary structure at the 3'end as well as RNAs with short poly A tails.
Phelan
D
D
Active Motif, Carlsbad, California, USA.
Hondorp
K
K
Choob
M
M
Efimov
V
V
Fernandez
J
J
D004247:DNA / Q000737:chemistry; D009690:Nucleic Acid Conformation; D020135:Peptide Nucleic Acids / Q000737:chemistry*; D011061:Poly A / Q000737:chemistry; D011071:Poly T / Q000737:chemistry; D012333:RNA, Messenger / Q000302:isolation & purification*
D020135:Peptide Nucleic Acids; D012333:RNA, Messenger; D011061:Poly A; D011071:Poly T; D004247:DNA