Sequences in the 5' leader of Mason-Pfizer monkey virus which affect viral particle production and genomic RNA packaging: development of MPMV packaging cell lines.
We used a series of deletion mutations in the 5' untranslated region of the prototype D type retrovirus, Mason-Pfizer Monkey Virus (MPMV), to analyse RNA encapsidation. A region was identified upstream of the major splice donor which reduced particle production but had a proportionally greater effect on RNA packaging. A small deletion downstream of the splice donor had little effect on RNA production and caused no significant packaging defect. A large deletion encompassing the end of the primer binding site down to the splice donor had a dramatic effect, disrupting viral protein synthesis. Stable cell lines were produced containing packaging-defective virus. These first-generation packaging cell lines were used to package and transfer an MPMV-based vector.
Guesdon
F M
FM
Department of Medicine, Addenbrooke's Hospital, University of Cambridge, Hills Road, Cambridge, CB2 2QQ, United Kingdom.
Greatorex
J
J
Rhee
S R
SR
Fisher
R
R
Hunter
E
E
Lever
A M
AM
D020121:5' Untranslated Regions / Q000737:chemistry / Q000235:genetics*; D000818:Animals; D019556:COS Cells; D002213:Capsid / Q000235:genetics / Q000378:metabolism; D002460:Cell Line; D002522:Chlorocebus aethiops; D006801:Humans; D007668:Kidney; D016093:Mason-Pfizer monkey virus / Q000235:genetics / Q000502:physiology*; D016296:Mutagenesis; D012367:RNA, Viral / Q000235:genetics*; D012194:RNA-Directed DNA Polymerase / Q000235:genetics / Q000378:metabolism; D017384:Sequence Deletion; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism; D014771:Virion / Q000235:genetics / Q000502:physiology; D014779:Virus Replication / Q000235:genetics*
D020121:5' Untranslated Regions; D012367:RNA, Viral; D014764:Viral Proteins; D012194:RNA-Directed DNA Polymerase