Expression of the simian varicella virus glycoprotein E.
Simian varicella virus (SVV) is closely related to human varicella-zoster virus (VZV) and induces a varicella-like disease in nonhuman primates. The SVV genome encodes a glycoprotein E (gE) which is homologous to the gE of VZV and other alphaherpesviruses. The SVV gE was expressed in Escherichia coli and rabbits were immunized with the recombinant gE fusion proteins to generate polyclonal gE antiserum. Immunofluorescence and immunoprecipitation analyses demonstrated that the SVV gE is expressed on the surface and within SVV-infected cells. The gE is also expressed on SVV virions as indicated by serum neutralization assay. The mature SVV gE is glycosylated and is similar in size ( approximately 100 kd) to the mature VZV gE. Immunohistochemical analysis detected gE within skin vesicles and lung tissue of SVV-infected monkeys. Analysis of the humoral immune response to gE in an SVV-infected monkey determined that anti-gE antibody is induced as early as day 9 postinfection and persists at high titer for longer than 4 months. The simian varicella model offers an opportunity to investigate the role of gE in viral pathogenesis and immunity and to evaluate its potential as a varicella vaccine.
Gray
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WL
Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, 4301 West Markham Street, Slot 511, Little Rock, AR 72205, USA. graywayne1@exchange.uams.edu
Mullis
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LB
Soike
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KF
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D000956:Antigens, Viral / Q000235:genetics* / Q000276:immunology; D002460:Cell Line; D002522:Chlorocebus aethiops; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D004926:Escherichia coli; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics; D000882:Haplorhini; D006566:Herpesviridae Infections / Q000097:blood / Q000276:immunology / Q000473:pathology / Q000821:virology*; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000276:immunology; D011817:Rabbits; D014616:Vaccinia virus / Q000235:genetics; D018140:Varicellovirus / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D014759:Viral Envelope Proteins / Q000235:genetics* / Q000276:immunology; D014764:Viral Proteins
D000914:Antibodies, Viral; D000956:Antigens, Viral; D008562:Membrane Glycoproteins; D014759:Viral Envelope Proteins; D014764:Viral Proteins; C062210:bacteriophage T7 RNA polymerase; D012321:DNA-Directed RNA Polymerases