title: Comparison of intracytoplasmic sperm injection for inbred and hybrid mice.
abstract: We compared the results of intracytoplasmic sperm injection (ICSI) that leads to full term development of hybrid (B6C3F1 and B6D2F1) and inbred (C57BL/6) mouse embryos. Although fertilization and pre-implantation development of C57BL/6 eggs were similar to those of F1 hybrid eggs, post-implantation development of the embryos from C57BL/6 females was significantly poorer than those of the eggs from hybrid females. Reciprocal crosses of C57BL/6 and B6C3F1 gametes revealed that the low rate of post-implantation development of C57BL/6 embryos was due to oocyte factor(s), rather than the sperm factor.
authors.affiliation: Pharmaceutical Technology Laboratory, Chugai Pharmaceutical Co., Ltd., Shizuoka, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Kawase
authors.forename: T
authors.initials: T
authors.lastname: Iwata
authors.forename: Y
authors.initials: Y
authors.lastname: Toyoda
authors.forename: T
authors.initials: T
authors.lastname: Wakayama
authors.forename: R
authors.initials: R
authors.lastname: Yanagimachi
authors.forename: H
authors.initials: H
authors.lastname: Suzuki
mesh_terms: D000818:Animals; D002462:Cell Membrane; D002470:Cell Survival; D003433:Crosses, Genetic; D004622:Embryo, Mammalian / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D005260:Female; D006824:Hybridization, Genetic; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D009865:Oocytes / Q000166:cytology* / Q000378:metabolism; D020554:Sperm Injections, Intracytoplasmic / Q000379:methods*; D013094:Spermatozoa / Q000378:metabolism / Q000637:transplantation*