Protein crystals in Phycomyces sporangiophores are involved in graviperception.
The sporangiophores of the zygomycete fungus Phycomyces blakesleeanus contain octahedral crystals with diameters of up to 5 micrometers in their vacuole. The crystals are associated with the intracellular membrane system. In tilted or horizontally placed sporangiophores, the crystals sediment to the respective lower face of the vacuole with a velocity of up to 100 micrometers per minute. The sedimentation is completed within about 2 minutes, well within the latency period for the negative gravitropic response of Phycomyces. Crystal-lacking mutant strains display a smaller maximal bending angle and a reduced gravitropic bending rate in comparison to the wild type. We therefore conclude that the crystals serve as statoliths for gravitropism in Phycomyces.
Schimek
C
C
Institute of Genetic Ecology, Tohoku University, Sendai, Japan.
Eibel
P
P
Horie
T
T
Galland
P
P
Ootaki
T
T
D003460:Crystallization; D005656:Fungal Proteins / Q000737:chemistry / Q000648:ultrastructure*; D018522:Gravitropism / Q000235:genetics / Q000502:physiology; D018466:Gravity Sensing / Q000502:physiology*; D009154:Mutation; D010800:Phycomyces / Q000737:chemistry / Q000235:genetics / Q000254:growth & development / Q000648:ultrastructure*; D014617:Vacuoles / Q000648:ultrastructure
D005656:Fungal Proteins