title: Ten years' experience in zygomycosis at a tertiary care centre in India.
abstract: OBJECTIVE
  To define the spectrum of zygomycosis due to mucorales in an Indian scenario.
  
  
  METHODS
  One-hundred and twenty-nine patients with zygomycosis due to mucorales diagnosed at the Postgraduate Institute of Medical Education and Research, Chandigarh, India during 1990-99, were retrospectively analysed regarding the sites of involvement, underlying disease, species of fungi isolated and outcome of therapy.
  
  
  RESULTS
  Higher prevalence rate (19.4%) was observed in 1999. Rhino-orbito-cerebral type (44.2%) was the commonest presentation followed by cutaneous (15.5%) and renal (14.0%) involvement. Disseminated zygomycosis was seen in 11.6% patients. Pulmonary and gastrointestinal zygomycosis were diagnosed in 10.1% and 4.7% patients, respectively. Uncontrolled diabetes mellitus (in 50% of cases) was the significant risk factor in rhino-orbito-cerebral type [odds ratio (OR), 9.3; P<or=0.001) and breach of skin (in 40% cases) in cutaneous zygomycosis (OR, 6.9; P<or=0.01). However, a considerable number of 22 (22.9%) patients were apparently healthy hosts in this series. Forty-five patients (34.9%) of this series were diagnosed only at post-mortem. Among 47 patients where culture was attempted, mucorales were isolated from 25 patients with Rhizopus arrhizus (11 patients) and Apophysomyces elegans (eight patients) as the predominant isolates. Adequate therapy could be provided in 33 patients. A combination of aggressive surgical debridement of necrotic tissue and amphotericin-B was found to be the best treatment protocol as 81.3% patients treated with surgical debridement and amphotericin-B were cured, compared with 46.7% patients treated with amphotericin-B alone.
  
  
  CONCLUSIONS
  The study highlights the importance of increased awareness for early diagnosis of zygomycosis and aggressive management. The large number of cases in apparently healthy hosts and increased isolation of A. elegans in the present series are important characteristics of this disease in India and requires further evaluation.
authors.affiliation: Department of Medical Microbiology, Postgraduate Institute of Medical Education and Research, Chandigarh-160012, India.
authors.forename: A
authors.initials: A
authors.lastname: Chakrabarti
authors.forename: A
authors.initials: A
authors.lastname: Das
authors.forename: A
authors.initials: A
authors.lastname: Sharma
authors.forename: N
authors.initials: N
authors.lastname: Panda
authors.forename: S
authors.initials: S
authors.lastname: Das
authors.forename: K L
authors.initials: KL
authors.lastname: Gupta
authors.forename: V
authors.initials: V
authors.lastname: Sakhuja
mesh_terms: D000293:Adolescent; D000328:Adult; D017677:Age Distribution; D000666:Amphotericin B / Q000627:therapeutic use; D000935:Antifungal Agents / Q000627:therapeutic use; D016009:Chi-Square Distribution; D002648:Child; D003646:Debridement; D005260:Female; D006801:Humans; D007194:India / Q000453:epidemiology; D008297:Male; D008499:Medical Records; D008875:Middle Aged; D009090:Mucorales / Q000302:isolation & purification; D016017:Odds Ratio; D012189:Retrospective Studies; D012233:Rhizopus / Q000302:isolation & purification; D012306:Risk; D012307:Risk Factors; D016019:Survival Analysis; D020096:Zygomycosis / Q000175:diagnosis* / Q000453:epidemiology / Q000382:microbiology / Q000628:therapy*
chemical_list: D000935:Antifungal Agents; D000666:Amphotericin B