Novel motility mutants of synechocystis strain PCC 6803 generated by in vitro transposon mutagenesis.
We screened for transposon-generated mutants of Synechocystis sp. strain PCC 6803 that exhibited aberrant phototactic movement. Of the 300 mutants generated, about 50 have been partially characterized; several contained transposons in genes encoding chemotaxis-related proteins, while others mapped to novel genes. These novel genes and their possible roles in motility are discussed.
Bhaya
D
D
Department of Plant Biology, Carnegie Institution, Stanford, California 94305, USA. devaki@andrew2.stanford.edu
Takahashi
A
A
Shahi
P
P
Grossman
A R
AR
D018528:ATP-Binding Cassette Transporters / Q000235:genetics; D000595:Amino Acid Sequence; D000458:Cyanobacteria / Q000502:physiology*; D004251:DNA Transposable Elements; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D018832:Molecular Chaperones / Q000235:genetics; D008969:Molecular Sequence Data; D009068:Movement / Q000502:physiology*; D016254:Mutagenesis, Insertional; D009154:Mutation; D017386:Sequence Homology, Amino Acid
D018528:ATP-Binding Cassette Transporters; D004251:DNA Transposable Elements; D029968:Escherichia coli Proteins; D018840:HSP70 Heat-Shock Proteins; D018832:Molecular Chaperones; C063842:dnaK protein, E coli