title: Neuronal regeneration in the cerebellum of adult teleost fish, Apteronotus leptorhynchus: guidance of migrating young cells by radial glia.
abstract: In contrast to mammals, adult fish exhibit an enormous potential to replace injured brain neurons by newly generated ones. In the present study, the role of radial glia, identified by immunostaining against fibrillary acidic protein (GFAP), was examined in this process of neuronal regeneration. Approximately 8 days after application of a mechanical lesion to the corpus cerebelli in the teleost fish Apteronotus leptorhynchus, the areal density of radial glial fibers increased markedly in the ipsilateral dorsal molecular layer compared to shorter survival times, or to the densities found in the intact brain or in the hemisphere contralateral to the lesion. This density remained elevated throughout the time period of up to 100 days examined. The increase in fiber density was followed approximately 2 days later by a rise in the areal density of young cells, characterized by labeling with the nuclear dye DAPI, in the ipsilateral dorsal molecular layer. Based on this remarkable spatio-temporal correlation, and the frequently observed close apposition of elongated young cells to radial glial fibers, we hypothesize that radial glia play an important role in the guidance of migrating young cells from their proliferation zones to the site of lesion where regeneration takes place.
authors.affiliation: School of Biological Sciences, University of Manchester, 3.614 Stopford Building, Oxford Road, Manchester M13 9PT, UK.
authors.forename: S C
authors.initials: SC
authors.lastname: Clint
authors.forename: G K
authors.initials: GK
authors.lastname: Zupanc
mesh_terms: D000367:Age Factors; D000818:Animals; D002450:Cell Communication / Q000502:physiology; D002465:Cell Movement / Q000502:physiology*; D002467:Cell Nucleus; D002531:Cerebellum / Q000166:cytology / Q000502:physiology*; D004555:Electric Fish; D005456:Fluorescent Dyes; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D007211:Indoles; D009416:Nerve Regeneration / Q000502:physiology*; D009457:Neuroglia / Q000737:chemistry / Q000166:cytology*; D009474:Neurons / Q000166:cytology*
chemical_list: D005456:Fluorescent Dyes; D005904:Glial Fibrillary Acidic Protein; D007211:Indoles; C007293:DAPI