Kinetically selective binding of single stranded RNA over DNA by a pyrrolidine-amide oligonucleotide mimic (POM).
Replacing the sugar-phosphodiester backbone of nucleic acids with a pyrrolidine-amide backbone results in an oligonucleotide mimic POM 1 which binds with high affinity and specificity to complementary DNA and RNA. Unlike other modified oligonucleotides, POM binds much more rapidly to single stranded RNA than DNA.
Hickman
D T
DT
Department of Chemistry, UMIST, PO Box 88, Manchester M60 1QD, United Kingdom.
King
P M
PM
Slater
J M
JM
Cooper
M A
MA
Micklefield
J
J
D000577:Amides / Q000737:chemistry / Q000378:metabolism; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D018716:Molecular Mimicry; D009841:Oligonucleotides / Q000737:chemistry* / Q000378:metabolism; D009994:Osmolar Concentration; D011759:Pyrrolidines / Q000737:chemistry* / Q000378:metabolism; D012313:RNA / Q000737:chemistry* / Q000378:metabolism; D013379:Substrate Specificity
D000577:Amides; D009841:Oligonucleotides; D011759:Pyrrolidines; D012313:RNA; D004247:DNA