title: Adaptations of human skeletal muscle fibers to spaceflight.
abstract: Human skeletal muscle fibers seem to share most of the same interrelationships among myosin ATPase activity, myosin heavy chain (MHC) phenotype, mitochondrial enzyme activities, glycolytic enzyme activities and cross-sectional area (CSA) as found in rat, cat and other species.  One difference seems to be that fast fibers with high mitochondrial content occur less frequently in humans than in the rat or cat.  Recently we have reported that the type of MHC expressed and the size of the muscle fibers in humans that have spent 11 days in space change significantly.  Specifically, about 8% more fibers express fast MHCs and all phenotypes atrophy in the vastus lateralis (VL) post compared to preflight.  In the present paper we examine the relationships among the population of myonuclei, MHC type and CSA of single human muscle fibers before and after spaceflight.  These are the first data that define the relationship among the types of MHC expressed, myonuclei number and myonuclei domain of single fibers in human muscle.  We then compare these data to similar measures in the cat.  In addition, the maximal torque that can be generated by the knee extensors and their fatigability before and after spaceflight are examined.  These data provide some indication of the potential physiological consequences of the muscle adaptations that occur in humans in response to spaceflight.
authors.affiliation: Department of Physiological Science, University of California, Los Angeles 90095, USA.
authors.forename: M K
authors.initials: MK
authors.lastname: Day
authors.forename: D L
authors.initials: DL
authors.lastname: Allen
authors.forename: L
authors.initials: L
authors.lastname: Mohajerani
authors.forename: M C
authors.initials: MC
authors.lastname: Greenisen
authors.forename: R R
authors.initials: RR
authors.lastname: Roy
authors.forename: V R
authors.initials: VR
authors.lastname: Edgerton
mesh_terms: D000222:Adaptation, Physiological; D000818:Animals; D001696:Biomechanical Phenomena; D002415:Cats; D002467:Cell Nucleus / Q000648:ultrastructure; D005260:Female; D006801:Humans; D008297:Male; D018613:Microscopy, Confocal; D018763:Muscle Fatigue / Q000502:physiology; D018485:Muscle Fibers, Skeletal / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure*; D018482:Muscle, Skeletal / Q000378:metabolism / Q000648:ultrastructure; D018995:Myosin Heavy Chains / Q000378:metabolism*; D051381:Rats; D013026:Space Flight; D013385:Succinate Dehydrogenase / Q000378:metabolism; D014893:Weightlessness
chemical_list: D013385:Succinate Dehydrogenase; D018995:Myosin Heavy Chains