Synthesis and hybridization of novel chiral pyrrolidine based PNA analogue.
Four different PNA fragments containing units of either the R- or S-isomer of N-(2-pyrrolidine-methyl)-N-(thymine-1-acetyl)-glycine (Pmg) were synthesized on a solid support. UV thermal melting experiments with complementary RNAs were performed and it was found that R-Pmg containing PNAs bind better to RNA than those containing the S-Pmg units.
Slaitas
A
A
Division of Organic and Bioorganic Chemistry, MBB, Scheele Laboratory, Karolinska Institute, S-17177 Stockholm, Sweden.
Yeheskiely
E
E
D005998:Glycine / Q000031:analogs & derivatives / Q000138:chemical synthesis* / Q000737:chemistry; D009693:Nucleic Acid Hybridization; D020135:Peptide Nucleic Acids / Q000138:chemical synthesis* / Q000737:chemistry; D011759:Pyrrolidines / Q000138:chemical synthesis* / Q000737:chemistry
C442886:N-(2-pyrrolidine-methyl)-N-(thymine-1-acetyl)-glycine; D020135:Peptide Nucleic Acids; D011759:Pyrrolidines; D005998:Glycine