Studies of a chemically modified oligodeoxynucleotide containing a 5-atom amide backbone which exhibits improved binding to RNA.
Chimeric oligodeoxyribonucleotides where the phosphodiester linkage -C3'-O-PO2--O-CH2-C4'- of DNA is substituted by the amide linkage -C3'-CH2-CH*(CH3)-CO-NH-CH2-C4' (*either R or S stereochemistry) have been prepared and their binding to RNA targets have been investigated. Incorporation of a single amide unit increases the Tm by approximately 1.4-1.9 degrees C. Circular dichroic spectra of these modified duplexes are similar to the wildtype DNA/RNA.
Wilds
C J
CJ
Department of Biological Sciences, Vanderbilt University, Nashville, Tennessee, USA.
Minasov
G
G
Natt
F
F
von Matt
P
P
Altmann
K H
KH
Egli
M
M
D000577:Amides / Q000737:chemistry*; D002942:Circular Dichroism; D009838:Oligodeoxyribonucleotides / Q000737:chemistry* / Q000378:metabolism; D012313:RNA / Q000737:chemistry* / Q000378:metabolism
D000577:Amides; D009838:Oligodeoxyribonucleotides; D012313:RNA