Xyloglucan from the leaves of Hymenaea courbaril.
A fucosylated xyloglucan was isolated from the leaves of Hymenaea courbaril by alkaline extraction, followed by ethanol precipitation and ion-exchange chromatography. The isolated polysaccharide showed Glc:Xyl:Gal:Fuc in molar ratio of 8:5:2.5:1 and (D)(25) +40.5 degrees. Composition and linkage analyses, supported by NMR spectroscopic measurements, showed that the polysaccharide has a glucan backbone which is highly substituted at O-6 with D-xylopyranose residues, about a half of which are substituted at O-2 by D-galactopyranosyl units. Some of the galactose residues are further substituted by L-fucopyranose at O-2. The M(r), as determined by HPSEC, was 49,500.
Busato
A P
AP
Departmento de Bioquímica, Universidade Federal do Paraná, CP 19046, 81531-990, Curitiba, Paraná, Brazil.
Vargas-Rechia
C G
CG
Reicher
F
F
D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D007887:Fabaceae / Q000737:chemistry*; D005936:Glucans; D009682:Magnetic Resonance Spectroscopy; D018515:Plant Leaves / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification*; D014990:Xylans
D005936:Glucans; D011134:Polysaccharides; D014990:Xylans; C029353:xyloglucan