Analysis of combinatorial chemistry samples by micellar electrokinetic chromatography.
A micellar electrokinetic chromatography (MEKC) method has been developed that can evaluate the purity of samples generated in combinatorial chemistry libraries. This method uses an open tube capillary (27 cm x 50 microm) along with a run buffer composed of sodium dodecyl sulfate (SDS), hydroxypropyl-beta-cyclodextrin, and sodium tetraborate coupled with UV detection. Neutral compounds and compounds that were insoluble in aqueous buffers could be analyzed under these conditions in approximately 3 min. The concentration of SDS and the concentration of hydroxypropyl-beta-cyclodextrin effected the separation. The affect on selectivity resulting from the addition of an organic modifier to the run buffer was examined. The low background absorbency of the run buffer made for easy detection of compounds that absorbed at low UV wavelengths. The quick analysis time made this suitable for analysis of combinatorial chemistry samples.
Simms
P J
PJ
Department of Chemistry, Chugai Biopharmaceuticals, Inc., 6275 Nancy Ridge Drive, San Diego, California 92121, USA. psimms@chugaibio.com
Jeffries
C T
CT
Huang
Y
Y
Zhang
L
L
Arrhenius
T
T
Nadzan
A M
AM
D002851:Chromatography, High Pressure Liquid; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D020650:Combinatorial Chemistry Techniques / Q000379:methods*; D007202:Indicators and Reagents; D013056:Spectrophotometry, Ultraviolet
D007202:Indicators and Reagents