The tomato mosaic tobamovirus movement protein interacts with a putative transcriptional coactivator KELP.
Viral movement through plasmodesmata in host plants likely depends on the interaction between virus-encoded movement protein (MP) and host proteins. In order to search for MP-interacting protein (MIP), we carried out far-western screening of a Brassica campestris cDNA library using a recombinant MP of tomato mosaic tobamovirus (ToMV) as a probe. One of the positive clones, designated MIP102, was found to be a putative orthologue for a transcriptional coactivator KELP of Arabidopsis thaliana. In vitro analysis with recombinant proteins revealed that ToMV MP could bind to KELP proteins that are derived from different plant species. At least 31 amino acids from the carboxyl-terminus of ToMV MP were dispensable for the interaction with KELP. Other MPs, derived from crucifer tobamovirus CTMV-W and cucumber mosaic cucumovirus, also exhibited comparable binding abilities. This suggests that these MPs could commonly interact with KELP, possibly to modulate the host gene expression.
Matsushita
Y
Y
Gene Research Center, Tokyo University of Agriculture and Technology, Japan.
Deguchi
M
M
Youda
M
M
Nishiguchi
M
M
Nyunoya
H
H
D000595:Amino Acid Sequence; D000818:Animals; D020346:Aquaporins; D029681:Arabidopsis Proteins; D001483:Base Sequence; D001937:Brassica / Q000235:genetics* / Q000821:virology; D015723:Gene Library; D006801:Humans; D007473:Ion Channels / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D053830:Plant Viral Movement Proteins; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D011485:Protein Binding; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D017898:Tobamovirus / Q000737:chemistry* / Q000378:metabolism; D015534:Trans-Activators / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
D020346:Aquaporins; D029681:Arabidopsis Proteins; D007473:Ion Channels; C113673:KELP protein, Arabidopsis; D010940:Plant Proteins; D053830:Plant Viral Movement Proteins; D011993:Recombinant Fusion Proteins; D015534:Trans-Activators; D014764:Viral Proteins; C110065:major intrinsic protein, plant; C491680:plasma membrane intrinsic protein 2 Arabidopsis