Novel propargylamine-linked nucleosides for high throughput SNP genotyping by MALDI-TOF MS.
The synthesis of positively charged and mass tagged nucleosides containing a quaternary ammonium functionality within the penultimate position of a primer is described. Neutralization of the sugar/thiophosphate backbone by alkylation increases the detection sensitivity in the mass spectrometric analysis by a factor of at least 100. The variable introduction of these novel compounds within the extension primers enables flexible design of multiplex genotyping reactions.
Wenzel
T
T
Bruker Saxonia Analytik GmbH, Permoserstr. 15, D-04318 Leipzig, Germany.
Fröhlich
T
T
Strassburger
K
K
Richter
S
S
Bimmler
J
J
Franke
C
C
Thomas
I
I
Kostrzewa
M
M
D004247:DNA / Q000737:chemistry / Q000235:genetics; D005838:Genotype; D006801:Humans; D009705:Nucleosides / Q000138:chemical synthesis* / Q000737:chemistry; D009943:Organophosphorus Compounds / Q000737:chemistry; D010293:Pargyline / Q000031:analogs & derivatives* / Q000737:chemistry*; D020641:Polymorphism, Single Nucleotide; D011437:Propylamines / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
D009705:Nucleosides; D009943:Organophosphorus Compounds; D011437:Propylamines; C434331:phosphoramidite; C009054:propargylamine; D004247:DNA; D010293:Pargyline