Cardiomyocyte-transistor-hybrids for sensor application.
An extracellular recording system has been designed for the detection of electrical cell signals using p-channel or n-channel field-effect transistor (FET) arrays with non-metallized gates. Signals from rat heart muscle cell were recorded by these devices and the results described on the basis of an equivalent circuit. This technique is sensitive enough to detect minute changes of the extracellular membrane voltage and has potential applications in drug screening. We show that known cardiac stimulants (isoproterenol, norepinephrine) and relaxants (verapamil, carbamylcholine) have characteristic effects on the heart cells in terms of the changes of beat frequencies in the absence or presence of corresponding agents.
Ingebrandt
S
S
Max Planck Institute for Polymer Research in Mainz, Ackermannweg 10, 55128 Mainz, Germany.
Yeung
C K
CK
Krause
M
M
Offenhäusser
A
A
D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation*; D002317:Cardiovascular Agents / Q000494:pharmacology; D002478:Cells, Cultured; D004353:Drug Evaluation, Preclinical; D006321:Heart / Q000187:drug effects; D009206:Myocardium / Q000166:cytology* / Q000378:metabolism; D051381:Rats; D014173:Transistors, Electronic
D002317:Cardiovascular Agents