title: Critical role of mast cells in morphine-mediated impairment of zymosan-induced peritonitis in mice.
abstract: OBJECTIVE
  Zymosan-induced peritoneal inflammation is significantly inhibited in mice injected with an irritant supplemented with morphine. The aim of the present study was to examine the putative mast cell involvement in this inhibition.
  
  
  METHODS
  Peritonitis was induced in WBB6FI mice (genetically mast cell-deficient W/Wv and their control littermaters +/+) and in Balb/c mice, with normal mast cells (MC) and mast cell-depleted (MCx) by pretreatment with compound 48/80. Bone marrow leukocytes from intact Balb/c mice were tested for their sensitivity to chemoattractants after in vitro incubation with morphine (10(-6) M), with or without preincubation with naltrexone (10(-8) M). Control cells were incubated in medium only.
  
  
  METHODS
  Peritonitis was induced by i.p. injection of either zymosan only (Z, 2 mg/ml) or zymosan supplemented with morphine (ZM, M: 20 mg/kg), without or with pretreatment with naltrexone (NZM, N: 5 mg/kg).
  
  
  METHODS
  Thirty minutes after induction of peritonitis, the histamine levels (ELISA) and vascular permeability (Evans blue leakage) were measured. At 6 h, the number of exudatory leukocytes (haemocytometer) and chemotaxis/chemoattractant level (48-well chemotactic chamber) were estimated.
  
  
  RESULTS
  (1) At 6 h of peritonitis, the number of exudatory leukocytes and levels of plasma chemoattractants were significantly lower in animals injected with zymosan supplemented with morphine (ZM) than in Z and NZM groups of WBB6F1 and Balb/c mice, but only in those with normal mast cells, and not in their mast cell-deficient/depleted counterparts. (2) In contrast, at 30 minutes, vascular permeability and histamine levels were higher in ZM than in Z group of mice with normal mast cells (MC), but not in those depleted of mast cells (MCx). (3) In vitro preincubation of leukocytes with morphine inhibited their migratory activity only towards peritoneal fluid from zymosan-treated MC mice but not from their MCx counterparts.
  
  
  CONCLUSIONS
  Mast cell-derived factors are involved in morphine-mediated impairment of zymosan-induced peritonitis in mice.
authors.affiliation: Department of Experimental Pathology, Institute of Medical Biology, University of Tromsø, Norway.
authors.forename: E
authors.initials: E
authors.lastname: Kolaczkowska
authors.forename: R
authors.initials: R
authors.lastname: Seljelid
authors.forename: B
authors.initials: B
authors.lastname: Plytycz
mesh_terms: D000701:Analgesics, Opioid / Q000494:pharmacology*; D000818:Animals; D000893:Anti-Inflammatory Agents; D001854:Bone Marrow Cells / Q000187:drug effects; D002199:Capillary Permeability / Q000187:drug effects; D002452:Cell Count; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D006632:Histamine / Q000378:metabolism; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008815:Mice, Inbred Strains; D009020:Morphine / Q000494:pharmacology*; D009271:Naltrexone / Q000494:pharmacology; D009292:Narcotic Antagonists / Q000494:pharmacology; D010538:Peritonitis / Q000139:chemically induced / Q000473:pathology*; D015054:Zymosan
chemical_list: D000701:Analgesics, Opioid; D000893:Anti-Inflammatory Agents; D009292:Narcotic Antagonists; D009271:Naltrexone; D009020:Morphine; D006632:Histamine; D015054:Zymosan