title: The gene encoding the immunoregulatory signaling molecule CMRF-35A localized to human chromosome 17 in close proximity to other members of the CMRF-35 family.
abstract: The immunoregulatory signaling (IRS) family includes several molecules, which play major roles in the regulation of the immune response. The CMRF-35A and CMRF-35H molecules are two new members of the IRS family of molecules, that are found on a wide variety of haemopoietic lineages. The extracellular functional interactions of these molecules is presently unknown, although CMRF-35H can initiate an inhibitory signal and is internalized when cross-linked. In this paper, we described the gene structure for the CMRF-35A gene and its localization to human chromosome 17. The gene consists of four exons spanning approximately 4.5 kb. Exon 1 encodes the 5' untranslated region and leader sequence, exon 2 encodes the immunoglobulin (Ig)-like domain, exon 3 encodes the membrane proximal region and exon 4 encodes the transmembrane region, the cytoplasmic tail and the 3' untranslated region. A region in the 5' flanking sequence of the CMRF-35A gene, that promoted expression of a reporter gene was identified. The genes for the CMRF-35A and CMRF-35H molecules are closely linked on chromosome 17. Similarity between the Ig-like exons and the preceding intron of the two genes suggests exon duplication was involved in their evolution. We also identified a further member of the CMRF-35 family, the CMRF-35J pseudogene. This gene appears to have arisen by gene duplication of the CMRF-35A gene. These three loci - the CMRF-35A, CMRF-35J and CMRF-35H genes-form a new complex of IRS genes on chromosome 17.
authors.affiliation: Mater Medical Research Institute, Aubigny Place, South Brisbane, Queensland, Australia. gclark@mmri.mater.org.au
authors.forename: G J
authors.initials: GJ
authors.lastname: Clark
authors.forename: B
authors.initials: B
authors.lastname: Cooper
authors.forename: S
authors.initials: S
authors.lastname: Fitzpatrick
authors.forename: B J
authors.initials: BJ
authors.lastname: Green
authors.forename: D N
authors.initials: DN
authors.lastname: Hart
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D020121:5' Untranslated Regions / Q000235:genetics; D000595:Amino Acid Sequence / Q000235:genetics; D000954:Antigens, Surface; D001483:Base Sequence / Q000235:genetics; D002874:Chromosome Mapping; D002886:Chromosomes, Human, Pair 17 / Q000235:genetics*; D006801:Humans; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics*; D011544:Pseudogenes / Q000276:immunology; D015398:Signal Transduction / Q000276:immunology*; D014407:Tumor Cells, Cultured; D020298:U937 Cells
chemical_list: D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D000954:Antigens, Surface; C444139:CD300C protein, human; D008562:Membrane Glycoproteins