High capacity substrates as a platform for a DNA probe array genotyping assay.
Colloidal silica particles were deposited on a glass substrate to produce high-capacity porous supports for high-density DNA probe arrays. Porous surfaces were used to increase the addressable surface area and number of probes available for hybridization. Surfaces derived from 70-100 nm size particles deposited in films from 0.15 to 2 microns thick exhibited excellent performance in light-directed oligonucleotide synthesis. Evaluation of these substrates in a genotyping assay is reported.
Fidanza
J
J
Affymetrix, Inc., Santa Clara, California, USA.
Glazer
M
M
Mutnick
D
D
McGall
G
G
Frank
C
C
D003102:Colloids; D015342:DNA Probes / Q000737:chemistry*; D004279:DNA, Viral / Q000235:genetics; D005838:Genotype; D005898:Glass / Q000737:chemistry*; D006678:HIV / Q000235:genetics; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D010316:Particle Size; D012822:Silicon Dioxide / Q000737:chemistry*; D013499:Surface Properties
D003102:Colloids; D015342:DNA Probes; D004279:DNA, Viral; D012822:Silicon Dioxide