title: A new continuous cell line from the mosquito Psorophora confinnis (Diptera: Culicidae) and its susceptibility to infections with some arboviruses.
abstract: A new cell line, PC-0199-BR, was established from embryonated eggs of the mosquito Psorophora confinnis. To date (September 2000) it has had 62 continuous passages. This is the first report of a cell line of mosquitoes belonging to the genus Psorophora. Cell growth initially was achieved in the MM/VP12 medium, supplemented with 20% fetal bovine serum; however, the subcultures were later adapted to Grace's medium with 10% fetal bovine serum. Cell morphology in the primary cultures was heterogeneous; but later in the established cell line, the predominant cell type was epithelioid. Cultured cells were predominantly diploid (2n=6); however, chromosome abnormalities were observed in a small proportion of the cells in later passages. C and G band patterns were also determined in the karyotype. The cell line isozyme profiles coincided with pupae and adult samples of the species taken from the same colony. A preliminary arbovirus susceptibility study for the cell line was undertaken. No evidence was observed of contamination of the cell line with bacteria, fungi or mycoplasma.
authors.affiliation: Departamento de Biología y Química, Universidad De La Salle, Bogotá, D.C., Colombia. fbellog@atenea.lasalle.edu.co
authors.forename: F J
authors.initials: FJ
authors.lastname: Bello
authors.forename: J A
authors.initials: JA
authors.lastname: Rodríguez
authors.forename: J
authors.initials: J
authors.lastname: Escovar
authors.forename: V A
authors.initials: VA
authors.lastname: Olano
authors.forename: A
authors.initials: A
authors.lastname: Morales
authors.forename: M
authors.initials: M
authors.lastname: González
authors.forename: G
authors.initials: G
authors.lastname: Rey
mesh_terms: D000818:Animals; D001103:Arboviruses; D002460:Cell Line / Q000737:chemistry / Q000166:cytology / Q000821:virology; D009033:Culicidae / Q000235:genetics* / Q000821:virology; D013997:Time Factors