Changes in hormonal balance and meristematic activity in primary root tips on the slowly rotating clinostat and their effect on the development of the rapeseed root system.
The morphometry of the root system, the meristematic activity and the level of indole-3-acetic acid (IAA), abscisic acid (ABA) and zeatin in the primary root tips of rapeseed seedlings were analyzed as functions of time on a  slowly rotating clinostat (1 rpm) or in the vertical controls (1 rpm). The fresh weight of the root system was 30% higher throughout the growth period (25 days) in clinorotated seedlings. Morphometric analysis showed that the increase in biomass on the clinostat was due to greater primary root growth, earlier initiation and greater elongation of the secondary roots, which could be observed even in 5-day-old seedlings. However, after 15 days, the growth of the primary root slowed on the clinostat, whereas secondary roots still grew faster in clinorotated plants than in the controls. At this time, the secondary roots began to be initiated closer to the root tip on the clinostat than in the control. Analysis of the meristematic activity and determination of the levels in IAA, ABA and zeatin in the primary root tips demonstrated that after 5 days on the clinostat, the increased length of the primary root could be the consequence of higher meristematic activity and coincided with an increase in both IAA and ABA concentrations. After 15 days on the clinostat, a marked increase in IAA, ABA and zeatin, which probably reached supraoptimal levels, seems to cause a progressive disturbance of the meristematic cells, during a decrease of primary root growth between 15 and 25 days. These modifications in the hormonal balance and the perturbation of the meristematic activity on the clinostat were followed by a loss of apical dominance, which was responsible for the early initiation of secondary roots, the greater elongation of the root system and the emergence of the lateral roots near the tip of the primary root.
Aarrouf
J
J
Laboratoire de Cytologie Experimentale et Morphogenese Vegetale, Universite Pierre et Marie Curie, Paris, France.
Schoevaert
D
D
Maldiney
R
R
Perbal
G
G
D000040:Abscisic Acid / Q000378:metabolism*; D018533:Biomass; D001937:Brassica / Q000254:growth & development / Q000502:physiology; D018744:DNA, Plant / Q000032:analysis; D006112:Gravitation; D007210:Indoleacetic Acids / Q000378:metabolism*; D018519:Meristem / Q000254:growth & development / Q000502:physiology*; D010937:Plant Growth Regulators / Q000378:metabolism*; D018518:Plant Root Cap / Q000254:growth & development / Q000502:physiology*; D018517:Plant Roots / Q000254:growth & development / Q000502:physiology; D012399:Rotation; D013997:Time Factors; D018474:Weightlessness Simulation; D015026:Zeatin / Q000378:metabolism*
D018744:DNA, Plant; D007210:Indoleacetic Acids; D010937:Plant Growth Regulators; C030737:indoleacetic acid; D000040:Abscisic Acid; D015026:Zeatin