Genetic characterization of Pseudomonas 'NZI7'--a novel pathogen that results in a brown blotch disease of Agaricus bisporus.
OBJECTIVE
To characterize a novel pseudomonad isolate capable of causing brown blotch disease of Agaricus bisporus.


RESULTS
Using the white-line-in-agar (WLA) assay, fluorescent pseudomonads isolated from a New Zealand mushroom farm were screened for the lipodepsipeptide tolaasin, a characteristic marker of Pseudomonas tolaasii. One isolate, NZI7, produced a positive WLA assay and caused brown lesions of A. bisporus comparable with those produced by Ps. tolaasii. However, genetic analysis suggested that Ps. tolaasii and NZI7 were genetically dissimilar, and that NZI7 is closely related to Pseudomonas syringae. Nucleotide sequence analyses of a gene involved in tolaasin production indicated that similar genes are present in both NZI7 and Ps. tolaasii.


CONCLUSIONS
NZI7 represents a novel Pseudomonas species capable of causing brown blotch disease of A. bisporus.


CONCLUSIONS
Phenotypic identification of Ps. tolaasii based on A. bisporus browning and positive WLA may have limited specificity.
Godfrey
S A
SA
University of Canterbury, Christchurch, New Zealand. godfreys@crop.cri.nz
Marshall
J W
JW
Klena
J D
JD
D000363:Agaricales; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics; D001427:Bacterial Toxins / Q000737:chemistry / Q000235:genetics; D018556:Crops, Agricultural / Q000382:microbiology; D005798:Genes, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011549:Pseudomonas / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity / Q000502:physiology*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D001426:Bacterial Proteins; D001427:Bacterial Toxins