title: Incorporation of positively charged deoxynucleic S-methylthiourea linkages into oligodeoxyribonucleotides.
abstract: Oligodeoxyribonucleic acids (15- and 18-mers) containing both negatively charged phosphate and positively charged S-methyl thiourea internucleoside linkages (DNmt/DNA chimera) have been synthesized. DNA binding characteristics and nuclease resistance of DNmt/DNA chimera have been evaluated.
authors.affiliation: Department of Chemistry and Biochemistry, University of California, Santa Barbara, 93106, USA.
authors.forename: H
authors.initials: H
authors.lastname: Challa
authors.forename: T C
authors.initials: TC
authors.lastname: Bruice
mesh_terms: D020137:Base Pair Mismatch; D001665:Binding Sites; D001671:Biochemistry / Q000379:methods*; D002942:Circular Dichroism; D005090:Exodeoxyribonucleases / Q000378:metabolism; D017403:In Situ Hybridization / Q000379:methods; D009838:Oligodeoxyribonucleotides / Q000737:chemistry* / Q000378:metabolism; D013890:Thiourea / Q000737:chemistry
chemical_list: D009838:Oligodeoxyribonucleotides; C120262:methylthioisourea; D005090:Exodeoxyribonucleases; C028450:exodeoxyribonuclease I; D013890:Thiourea