Identification, characterization, and functional analysis of a gene encoding the ferric uptake regulation protein in Bartonella species.
Environmental iron concentrations coordinately regulate transcription of genes involved in iron acquisition and virulence via the ferric uptake regulation (fur) system. We identified and sequenced the fur gene and flanking regions of three Bartonella species. The most notable difference between Bartonella Fur and other Fur proteins was a substantially higher predicted isoelectric point. No promoter activity or Fur autoregulation was detected using a gfp reporter gene fused to the 204 nucleotides immediately upstream of the Bartonella fur gene. Bartonella henselae fur gene expression complemented a Vibrio cholerae fur mutant.
Park
S Y
SY
Division of Infectious Diseases, Department of Pediatrics, University of California-San Francisco, 94143-0654, USA.
Kelminson
K L
KL
Lee
A K
AK
Zhang
P
P
Warner
R E
RE
Rehkopf
D H
DH
Calderwood
S B
SB
Koehler
J E
JE
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D001473:Bartonella / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D015139:Blotting, Southern; D015964:Gene Expression Regulation, Bacterial; D005816:Genetic Complementation Test; D006801:Humans; D007501:Iron / Q000378:metabolism; D008969:Molecular Sequence Data; D012097:Repressor Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D014734:Vibrio cholerae / Q000235:genetics / Q000378:metabolism
D001426:Bacterial Proteins; D012097:Repressor Proteins; C068477:ferric uptake regulating proteins, bacterial; D007501:Iron