A novel organization of the dissimilatory sulfite reductase operon of Thermodesulforhabdus norvegica verified by RT-PCR.
The nucleotide sequence of the gene cluster for the dissimilatory sulfite reductase (Dsr) from the Gram-negative thermophilic sulfate reducer Thermodesulforhabdus norvegica was determined. The Dsr-encoding genes (dsrAB) were found to be located in an operon encompassing four other open reading frames in the following order: dsrD-dsrA-dsrB-dsrN-dsrC-fdx. Localization of these six genes in the same operon supports previous suggestions that dsrD, -C and -N play essential roles in the mechanism for reduction of sulfite to sulfide. Transcriptional analysis showed that these genes constitute a contiguous transcriptional unit of at least 5 kb. The phylogeny of Dsr is discussed.
Larsen Ø
Department of Microbiology, University of Bergen, Jahnebakken 5, P.O. Box 7800, N-5020 Bergen, Norway.
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D003001:Cloning, Molecular; D020564:Deltaproteobacteria / Q000201:enzymology / Q000235:genetics*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D005810:Multigene Family; D009876:Operon; D050862:Oxidoreductases Acting on Sulfur Group Donors / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction
D050862:Oxidoreductases Acting on Sulfur Group Donors