title: Effect of fluid and salt supplements in preventing the development of "osteopenia" in hypokinetic rats.
abstract: It has been suggested that a daily intake of fluid and salt supplements may be used to prevent bone demineralization in human subjects after prolonged exposure to hypokinesia (diminished muscular activity). Thus, the objective of this investigation was to evaluate the effect of fluid and salt supplementation in the prevention of development of osteoporosis in 64 Wistar rats with an initial body weight of 339-345 g, after exposure to 90 days of hypokinesia. They divided into 4 equal groups: the first group of rats placed under ordinary vivarium conditions and served as vivarium control; the second group were also placed under ordinary vivarium conditions but received daily fluid and salt supplements; the third group were subjected to pure hypokinesia, i.e. without the use of any preventive measures; and the fourth group were submitted to hypokinesia and received daily fluid and salt supplements. For the simulation of the hypokinetic effect the experimental group of rats were kept in small, individual, wooden cages. Through the experimental period the second and fourth group of rats received 8 ml/100 g body wt water and 5 ml 100 g body wt NaCl daily. By the end of the experimental period the animals were decapitated and the spongy matter of tibia and vertebrae of the rats were examined for changes referable to osteoporosis. It was found that the daily intake of fluid and salt supplements caused an increase in the volume density of primary spongiosa of bones. It was concluded that a daily intake of fluid and salt supplements may be used to prevent the development of osteoporosis in rats subjected to prolonged motor activity restriction.
authors.affiliation: Medical Engineering Institute, Tokyo, Japan.
authors.forename: Y G
authors.initials: YG
authors.lastname: Zorbas
authors.forename: Y F
authors.initials: YF
authors.lastname: Federenko
authors.forename: M N
authors.initials: MN
authors.lastname: Togawa
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects; D018488:Bone Demineralization, Pathologic / Q000517:prevention & control*; D001846:Bone Development / Q000187:drug effects*; D001842:Bone and Bones / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D004326:Drinking; D005069:Evaluation Studies as Topic; D005440:Fluid Therapy; D018476:Hypokinesia; D007103:Immobilization; D008297:Male; D010024:Osteoporosis / Q000517:prevention & control*; D051381:Rats; D017208:Rats, Wistar; D012965:Sodium Chloride / Q000494:pharmacology* / Q000627:therapeutic use; D013623:Tail / Q000033:anatomy & histology / Q000187:drug effects / Q000254:growth & development
chemical_list: D012965:Sodium Chloride