Characterization of a sea bream (Sparus aurata) thyroid hormone receptor-beta clone expressed during embryonic and larval development.
A clone encoding thyroid hormone receptor-beta (TR-beta) was isolated from a sea bream (Sparus aurata) ovary cDNA library. Sea bream (sb)TR-beta is closely related to its counterparts from other vertebrates and, like them, preferentially binds T3 rather than T4. However, the putative sbTR-beta protein contains a nine-amino-acid insert that is also present in the corresponding proteins from flounder and salmon but absent in TR-betas from zebrafish and terrestrial vertebrates. Semiquantitative RT-PCR analysis showed that sbTR-beta transcripts begin to accumulate during gastrulation and increase markedly in quantity up to the period around hatch (ca. 40 h postfertilization) before declining slightly. In adult tissues, TR-beta mRNA was present in approximately equal quantities in heart, intestine, brain, kidney, skeletal muscle, liver, and gill. The significance of the relatively strong expression of TR-beta during sea bream embryogenesis is discussed.
Nowell
M A
MA
Cardiff School of Biosciences, Cardiff University, United Kingdom.
Power
D M
DM
Canario
A V
AV
Llewellyn
L
L
Sweeney
G E
GE
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D047108:Embryonic Development; D005260:Female; D015870:Gene Expression; D015723:Gene Library; D007814:Larva / Q000254:growth & development*; D008969:Molecular Sequence Data; D010053:Ovary / Q000737:chemistry; D011988:Receptors, Thyroid Hormone / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D021541:Sea Bream / Q000235:genetics; D016415:Sequence Alignment; D013974:Thyroxine / Q000378:metabolism; D014284:Triiodothyronine / Q000378:metabolism
D018076:DNA, Complementary; D011988:Receptors, Thyroid Hormone; D014284:Triiodothyronine; D013974:Thyroxine