Specific reduction of wheat storage proteins by thioredoxin h.
Gliadins and glutenins, the major storage proteins of wheat endosperm (Triticum durum, Desf. cv Monroe), were reduced in vitro by the NADP/thioredoxin system (NADPH, NADP-thioredoxin reductase and thioredoxin; in plants, the h type) from either the same source or the bacterium Escherichia coli.  A more limited reduction of certain members of these protein groups was achieved with the reduced form of glutathione or glutaredoxin, a protein known to replace thioredoxin in certain bacterial and mammalian enzyme systems but not known to occur in higher plants.  Endosperm extracts contained the enzymes necessary to reduce NADP by the oxidative pentose phosphate pathway (hexokinase, glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase).  The gliadins and glutenins were also reduced in vivo during germination--an event that accompanied their proteolytic breakdown.  The results suggest that thioredoxin, reduced by NADPH generated via the oxidative pentose phosphate pathway, functions as a signal in germination to enhance metabolic processes such as the mobilization of storage proteins and, as found earlier, the activation of enzymes.
Kobrehel
K
K
Department of Plant Biology, University of California, Berkeley 94720, USA.
Wong
J H
JH
Balogh
A
A
Kiss
F
F
Yee
B C
BC
Buchanan
B B
BB
D018525:Germination / Q000502:physiology*; D005903:Gliadin / Q000032:analysis / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism; D005983:Glutens / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism; D009249:NADP / Q000032:analysis / Q000378:metabolism / Q000502:physiology; D010084:Oxidation-Reduction; D010940:Plant Proteins / Q000032:analysis / Q000378:metabolism / Q000502:physiology*; D012639:Seeds / Q000201:enzymology / Q000502:physiology; D054480:Thioredoxin h; D013879:Thioredoxins / Q000032:analysis / Q000378:metabolism*; D014908:Triticum / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*
D010940:Plant Proteins; D054480:Thioredoxin h; D013879:Thioredoxins; D009249:NADP; D005983:Glutens; D005903:Gliadin; C037302:glutenin; D005978:Glutathione