Densitometric analysis of nuclear DNA content in lentil roots grown in space.
Lentil seedlings were grown for 28 h in space, on board Spacelab (IML 1 Mission) and growth of the primary root was analysed. The length of cortical cells was less in near weightlessness than on the 1 g centrifuge (flight control) and mitotic index was lower but there was no apparent perturbation in the mitosis. To further investigate which phase of cell cycle was modified, densitometric analysis of nuclear DNA content in cortical cells was carried out by the mean of an image processing system (SAMBA). In microgravity there was a decrease in DNA synthesis and a promotion of the arrest in the G2 phase of cell cycle. These results, and other ones obtained elsewhere on a slowly rotating clinostat, led us to think that in microgravity the perturbation of the gravisensing cells and/or the absence of convection could account for the modification of cell growth registered in the primary root.
Driss-Ecole
D
D
Universite Pierre et Marie Curie, Paris, France.
Schoevaert
D
D
Noin
M
M
Perbal
G
G
D019149:Bioreactors; D002453:Cell Cycle / Q000502:physiology; D002467:Cell Nucleus / Q000235:genetics*; D002498:Centrifugation; D018744:DNA, Plant; D003720:Densitometry; D007887:Fabaceae / Q000166:cytology* / Q000235:genetics / Q000254:growth & development; D008938:Mitosis; D008940:Mitotic Index; D016011:Normal Distribution; D018517:Plant Roots / Q000166:cytology* / Q000235:genetics / Q000254:growth & development; D010946:Plants, Medicinal; D013026:Space Flight; D014893:Weightlessness
D018744:DNA, Plant