title: Light-induced proton release and proton uptake reactions in the cyanobacterial phytochrome Cph1.
abstract: The P(r) to P(fr) transition of recombinant Synechocystis PCC 6803 phytochrome Cph1 and its N-terminal sensor domain Cph1Delta2 is accompanied by net acidification in unbuffered solution. The extent of this net photoreversible proton release was measured with a conventional pH electrode and increased from less than 0.1 proton released per P(fr) formed at pH 9 to between 0.6 (Cph1) and 1.1 (Cph1Delta2) H(+)/P(fr) at pH 6. The kinetics of the proton release were monitored at pH 7 and pH 8 using flash-induced transient absorption measurements with the pH indicator dye fluorescein. Proton release occurs with time constants of approximately 4 and approximately 20 ms that were also observed in parallel measurements of the photocycle (tau(3) and tau(4)). The number of transiently released protons per P(fr) formed is about one. This H(+) release phase is followed by a proton uptake phase of a smaller amplitude that has a time constant of approximately 270 ms (tau(5)) and is synchronous with the formation of P(fr). The acidification observed in the P(r) to P(fr) transition with pH electrodes is the net effect of these two sequential protonation changes. Flash-induced transient absorption measurements were carried out with Cph1 and Cph1Delta2 at pH 7 and pH 8. Global analysis indicated the presence of five kinetic components (tau(1)-tau(5): 5 and 300 micros and 3, 30, and 300 ms). Whereas the time constants were approximately pH independent, the corresponding amplitude spectra (B(1), B(3), and B(5)) showed significant pH dependence. Measurements of the P(r)/P(fr) photoequilibrium indicated that it is pH independent in the range of 6.5-9.0. Analysis of the pH dependence of the absorption spectra from 6.5 to 9.0 suggested that the phycocyanobilin chromophore deprotonates at alkaline pH in both P(r) and P(fr) with an approximate pK(a) of 9.5. The protonation state of the chromophore at neutral pH is therefore the same in both P(r) and P(fr). The light-induced deprotonation and reprotonation of Cph1 at neutral pH are thus due to pK(a) changes in the protein moiety, which are linked to conformational transitions occurring around 4 and 270 ms after photoexcitation. These transient structural changes may be relevant for signal transduction by this cyanobacterial phytochrome.
authors.affiliation: Laboratory for Microbiology, Swammerdam Institute for Life Sciences, University of Amsterdam, Nieuwe Achtergracht 166, 1018 WV Amsterdam, The Netherlands. jasper@biop.ox.ac.uk
authors.forename: J J
authors.initials: JJ
authors.lastname: van Thor
authors.forename: B
authors.initials: B
authors.lastname: Borucki
authors.forename: W
authors.initials: W
authors.lastname: Crielaard
authors.forename: H
authors.initials: H
authors.lastname: Otto
authors.forename: T
authors.initials: T
authors.lastname: Lamparter
authors.forename: J
authors.initials: J
authors.lastname: Hughes
authors.forename: K J
authors.initials: KJ
authors.lastname: Hellingwerf
authors.forename: M P
authors.initials: MP
authors.lastname: Heyn
mesh_terms: D001426:Bacterial Proteins; D000458:Cyanobacteria / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008027:Light; D016296:Mutagenesis; D010777:Photochemistry; D020283:Photoreceptors, Microbial; D010834:Phytochrome / Q000737:chemistry / Q000378:metabolism* / Q000528:radiation effects*; D011494:Protein Kinases / Q000737:chemistry / Q000378:metabolism* / Q000528:radiation effects*; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism / Q000528:radiation effects; D017384:Sequence Deletion; D012996:Solutions; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry
chemical_list: D001426:Bacterial Proteins; D020283:Photoreceptors, Microbial; D011994:Recombinant Proteins; D012996:Solutions; D010834:Phytochrome; D011494:Protein Kinases; C413623:Cph1 phytochrome protein, bacteria