title: MIP-2 secreted by epithelial cells increases neutrophil and lymphocyte recruitment in the mouse intestine.
abstract: BACKGROUND
  Invasion of the intestinal mucosa by leucocytes is a characteristic of intestinal inflammation but the role of the epithelium in orchestrating this recruitment has not been examined in vivo. Cultured intestinal epithelial cells secrete a wide variety of chemokines, often in response to agents present in the intestinal lumen. Macrophage inflammatory protein 2 (MIP-2) is a chemokine that attracts neutrophils, and its secretion from intestinal epithelial cells is enhanced by inflammatory stimuli such as interleukin 1beta. We hypothesised that the production of MIP-2 by epithelial cells would increase leucocyte migration into the intestine.
  
  
  OBJECTIVE
  To study the effects of a chemokine secreted from intestinal epithelial cells in vivo.
  
  
  METHODS
  MIP-2 was expressed in the mouse intestinal epithelium using an epithelial cell specific promoter from the gene encoding the intestinal fatty acid binding protein. The intestines of these transgenic mice were then analysed.
  
  
  RESULTS
  Epithelial cells from transgenic mice expressed MIP-2 but wild-type mice did not. Neutrophil recruitment, examined by myeloperoxidase (MPO) staining and total MPO activity per unit weight of intestine, was significantly increased in transgenic mice in both the small intestine and proximal colon, and this was blocked by anti-MIP-2 antibody treatment. Both intraepithelial and lamina propria lymphocytes were also increased in transgenic mice. They showed chemotactic activity to MIP-2 in the Boyden chambers and expressed MIP-2 receptor (CXCR-2) mRNA confirmed by reverse transcription-polymerase chain reaction.
  
  
  CONCLUSIONS
  These experiments are the first to show a functional role for epithelial chemokines in vivo and reveal an unexpected role for the neutrophil chemokine MIP-2 in controlling mucosal lymphocyte migration.
authors.affiliation: Department of Adult and Paediatric Gastroenterology, St Bartholomew's and the Royal London School of Medicine and Dentistry, London, UK.
authors.forename: Y
authors.initials: Y
authors.lastname: Ohtsuka
authors.forename: J
authors.initials: J
authors.lastname: Lee
authors.forename: D S
authors.initials: DS
authors.lastname: Stamm
authors.forename: I R
authors.initials: IR
authors.lastname: Sanderson
mesh_terms: D000818:Animals; D054426:Chemokine CXCL2; D019743:Chemokines, CXC; D002634:Chemotaxis, Leukocyte / Q000502:physiology*; D004848:Epithelium / Q000276:immunology / Q000378:metabolism; D007111:Immunity, Cellular; D036341:Intercellular Signaling Peptides and Proteins; D007413:Intestinal Mucosa / Q000378:metabolism*; D008214:Lymphocytes / Q000502:physiology*; D051379:Mice; D008822:Mice, Transgenic; D015846:Monokines / Q000378:metabolism*; D020556:Neutrophil Infiltration / Q000502:physiology*; D009195:Peroxidase / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D019707:Receptors, Chemokine / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013194:Staining and Labeling / Q000379:methods
chemical_list: C499706:CXCL2 protein, human; D054426:Chemokine CXCL2; D019743:Chemokines, CXC; D036341:Intercellular Signaling Peptides and Proteins; D015846:Monokines; D012333:RNA, Messenger; D019707:Receptors, Chemokine; D009195:Peroxidase