Recombinant bacterial cells as efficient biocatalysts for the production of nucleosides.
The preparation of nucleosides as well as their base-modified analogues using purified nucleoside phosphorylase enzymes or, more conveniently, using whole bacterial cells is described. The development of genetically modified strains of Escherichia coli, able to over-produce Uridine-phosphorylase and Purine-nucleoside-phosphorylase in the same cells, improves the specific biocatalytic activity and the consequent industrial scale approach.
Spoldi
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E
Dipartimento di Genetica e Biologia dei Microorganismi, University of Milan, Via Celoria 26, 20133 Milan, Italy.
Ghisotti
D
D
Calì
S
S
Grisa
M
M
Orsini
G
G
Tonon
G
G
Zuffi
G
G
D004926:Escherichia coli / Q000201:enzymology / Q000378:metabolism*; D009705:Nucleosides / Q000096:biosynthesis*; D011683:Purine-Nucleoside Phosphorylase / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D014543:Uridine Phosphorylase / Q000378:metabolism*; D014740:Vidarabine / Q000096:biosynthesis
D009705:Nucleosides; D011994:Recombinant Proteins; D011683:Purine-Nucleoside Phosphorylase; D014543:Uridine Phosphorylase; D014740:Vidarabine