An oleate desaturase and a suppressor loci direct high oleic acid content of sunflower (Helianthus annuus L.) oil in the Pervenets mutant.
All the [HOAC] lines derived from the Pervenets mutant carry a specific RFLP (oleHOS) revealed by an oleate desaturase cDNA used as a probe. The [LO] (linoleic) genotypes do not carry oleHOS, but another allele: oleLOR. We studied [HOAC] heredity in two segregating populations. In an F2 population, the [HOAC] trait co-segregated with oleHOS. In a recombinant inbred line F6 population, all [HOAC] RI lines carried oleHOS. The RI lines carrying oleHOS were either [LO] or [HOAC]. The absence of [HOAC] RI lines with oleLOR eliminated the occurrence of a recombination event between the locus carrying oleHOS and the locus carrying Pervenets allele. The [HOAC] trait is due to 2 independent loci: the locus carrying oleHOS allele and another locus. One allele at this other locus may suppress the effect of oleHOS allele on the [HOAC] trait. The existence of this suppressor allele has only been suggested for sunflower.
Lacombe
S
S
INRA, UMR 1097, UR-GAP, Bât 33, 2, place P.-Viala, 34060 Montpellier, 1, France.
Kaan
F
F
Léger
S
S
Bervillé
A
A
D000483:Alleles; D003433:Crosses, Genetic; D015342:DNA Probes; D018076:DNA, Complementary; D044943:Fatty Acid Desaturases / Q000235:genetics* / Q000378:metabolism*; D005838:Genotype; D006368:Helianthus / Q000201:enzymology* / Q000235:genetics*; D009154:Mutation; D019301:Oleic Acid / Q000032:analysis*; D044925:Oxidoreductases Acting on CH-CH Group Donors; D010938:Plant Oils / Q000737:chemistry*; D011995:Recombination, Genetic; D013489:Suppression, Genetic
D015342:DNA Probes; D018076:DNA, Complementary; D010938:Plant Oils; D019301:Oleic Acid; D044943:Fatty Acid Desaturases; C021904:Phosphatidylcholine desaturase; D044925:Oxidoreductases Acting on CH-CH Group Donors