Production and action of cytokines in space.
B6MP102 cells, a continuously cultured murine bone marrow macrophage cell line, were tested for secretion of tumor necrosis factor-alpha and Interleukin-1 during space flight.  We found that B6MP102 cells secreted more tumor necrosis factor-alpha and interleukin-1 when stimulated in space with lipopolysaccharide than controls similarly stimulated on earth.  This compared to increased secretion of interferon-beta and -gamma by lymphocytes that was measured on the same shuttle flights.  Although space flight enhanced B6MP102 secretion of tumor necrosis factor-alpha, an experiment on a subsequent space flight (STS-50) found that cellular cytotoxicity, mediated by tumor necrosis factor-alpha, was inhibited.
Chapes
S K
SK
Division of Biology, Kansas State University, Manhattan 66506.
Morrison
D R
DR
Guikema
J A
JA
Lewis
M L
ML
Spooner
B S
BS
D000818:Animals; D001853:Bone Marrow / Q000378:metabolism / Q000502:physiology; D001854:Bone Marrow Cells; D002460:Cell Line; D016207:Cytokines / Q000378:metabolism* / Q000502:physiology*; D003602:Cytotoxicity, Immunologic; D016899:Interferon-beta / Q000378:metabolism / Q000502:physiology; D007371:Interferon-gamma / Q000378:metabolism / Q000502:physiology; D007375:Interleukin-1 / Q000378:metabolism / Q000502:physiology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D051379:Mice; D013026:Space Flight; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism / Q000494:pharmacology / Q000502:physiology*; D014893:Weightlessness
D016207:Cytokines; D007375:Interleukin-1; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D016899:Interferon-beta; D007371:Interferon-gamma