title: Exploring the properties of thermostable Clostridium thermocellum cellulase CelE for the purpose of its expression in plants.
abstract: The main properties (pH and temperature range, stability, substrate specificity) of the modified cellulase CelE (endo-beta-1,4-glucanase) from Clostridium thermocellum have been analyzed with the goal of its expression in plants. The modified enzyme is similar to plant cellulases. Deletions in the N-terminus of the enzyme do not affect its biochemical properties. Based on the present investigation, we conclude that the modified beta-1,4-glucanase CelEM1, when expressed in plants, will be a good model to study the role of cellulases in plants.
authors.affiliation: Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, 119991, Russia. roustam@vigg.ru
authors.forename: R M
authors.initials: RM
authors.lastname: Abdeev
authors.forename: I V
authors.initials: IV
authors.lastname: Goldenkova
authors.forename: K A
authors.initials: KA
authors.lastname: Musiychuk
authors.forename: E S
authors.initials: ES
authors.lastname: Piruzian
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002266:Carboxymethylcellulose Sodium / Q000378:metabolism*; D002480:Cellulase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D003013:Clostridium / Q000201:enzymology* / Q000235:genetics; D003198:Computer Simulation; D004789:Enzyme Activation; D015870:Gene Expression / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D005936:Glucans / Q000378:metabolism*; D008957:Models, Genetic; D009154:Mutation / Q000235:genetics; D010944:Plants / Q000201:enzymology / Q000235:genetics; D017384:Sequence Deletion / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D005936:Glucans; D002480:Cellulase; D002266:Carboxymethylcellulose Sodium; C015968:lichenin