title: RNA and N3'-->P5' kissing aptamers targeted to the trans-activation responsive (TAR) RNA of the human immunodeficiency virus-1.
abstract: We used in vitro selection to identify RNA aptamers able to selectively bind to the TAR RNA motif of HIV-1, an unperfect RNA hairpin involved in the transcription of the retroviral genome. We selected aptameric RNA hairpins giving rise to kissing complexes with TAR. The N3'-->P5' phosphoramidate variant of the aptamer bind to TAR with a Kd in the low nanomolar range. However, only the RNA-RNA loop-loop complex is recognized by the Rop protein of E. coli which is specific for kissing complexes.
authors.affiliation: INSERM U386, Université Victor Segalen, Bordeaux, France.
authors.forename: F
authors.initials: F
authors.lastname: Darfeuille
authors.forename: C
authors.initials: C
authors.lastname: Cazenave
authors.forename: S
authors.initials: S
authors.lastname: Gryaznov
authors.forename: F
authors.initials: F
authors.lastname: Ducongé
authors.forename: C
authors.initials: C
authors.lastname: Di Primo
authors.forename: J J
authors.initials: JJ
authors.lastname: Toulmé
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D016325:HIV Long Terminal Repeat / Q000235:genetics*; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000235:genetics / Q000494:pharmacology; D012313:RNA / Q000235:genetics* / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D020218:Response Elements / Q000235:genetics; D013379:Substrate Specificity; D020349:Surface Plasmon Resonance; D015533:Transcriptional Activation
chemical_list: D001426:Bacterial Proteins; D009841:Oligonucleotides; D016601:RNA-Binding Proteins; C054243:Rop protein, ColE1 plasmid; D012313:RNA