Development of circadian rhythms in rat pups exposed to microgravity during gestation.
Ten pregnant Sprague Dawley rat dams were exposed to spaceflight aboard the Space Shuttle (STS-70) for gestational days 11-20 (G 11-20; FLT group). Control dams were maintained in either a flight-like (FDS group) or vivarium cage environment (VIV group) on earth. All dams had ad lib access to food and water and were exposed to a light-dark cycle consisting of 12 hours of light (approximately 30 lux) followed by 12 hours of darkness. The dams were closely monitored from G 22 until parturition. All pups were cross-fostered at birth; each foster dam had a litter of 10 pups. Pups remained with their foster dam until post natal day 21 (PN 21). Pup body mass was measured twice weekly. At PN 14 FLT pups had a smaller body mass than did the VIV pups (p < 0.01). Circadian rhythms of body temperature and activity of pups from two FLT dams (n = 8), two FDS dams (n = 9) and two VIV dams (n = 7) were studied starting from age PN 21. All pups had circadian rhythms of temperature and activity at this age. There were no significant differences in rhythms between groups that could be attributed to microgravity exposure. These results indicate that exposure to the microgravity environment of spaceflight during this embryonic development period does not affect the development of the circadian rhythms of body temperature and activity.
Hoban-Higgins
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TM
Section of Neurobiology, Physiology, & Behavior, University of California, Davis 95616-8519, USA. tmhoban@ucdavis.edu
Murakami
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DM
Tang
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Fuller
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PM
Fuller
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D000818:Animals; D000831:Animals, Newborn; D001831:Body Temperature; D001835:Body Weight; D002940:Circadian Rhythm / Q000502:physiology*; D005260:Female; D008297:Male; D009043:Motor Activity; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D013026:Space Flight; D013997:Time Factors; D014893:Weightlessness