Identification of alternative splice products encoded by the human coxsackie-adenovirus receptor gene.
The human cellular receptor for group B coxsackieviruses and adenoviruses (HCAR) is a transmembrane glycoprotein which belongs to the immunoglobulin superfamily. We describe alternative splicing of the HCAR-gene and the existence of three exon-skipping splice variants in addition to the originally identified seven exon-encompassing mRNA transcript. Expression of the splice variants theoretically results in truncated proteins, possibly leading to impaired viral binding and/or the occurrence of soluble viral receptors due to the absence of the transmembranous region. Consequently, this could markedly influence the efficacy of an adenovirus subgroup C-mediated gene therapy.
Thoelen
I
I
Laboratory of Clinical & Epidemiological Virology, Rega Institute for Medical Research, Minderbroedersstraat 10, BE-3000 Leuven, Belgium.
Magnusson
C
C
Tågerud
S
S
Polacek
C
C
Lindberg
M
M
Van Ranst
M
M
D000256:Adenoviridae / Q000378:metabolism; D017398:Alternative Splicing; D000595:Amino Acid Sequence; D001483:Base Sequence; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D004770:Enterovirus / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000378:metabolism; D011991:Receptors, Virus / Q000235:genetics*
C568771:CLMP protein, human; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D012333:RNA, Messenger; D011991:Receptors, Virus