[Initial experience with detection of Saccharomyces cerevisiae antibodies in patients with primary nonspecific inflammatory bowel disease].
BACKGROUND
Inflammatory bowel diseases (IBD), with Crohn's disease (CD) and ulcerative colitis (UC) as the two main disorders, is a heterogeneous group of diseases of unknown etiology. Actually we have no ideal disease marker, to identify people at risk of the disease, which can differentiate CD from UC, be highly specific for CD or UC and easily applicable in routine laboratory praxis.


OBJECTIVE
Determine the clinical significance of serological testing p-ANCA and ASCA in patients with IBD.


METHODS
P-ANCA in IgG isotype were detected by indirect fluorescence assay on human ethanol-fixed granulocytes, ASCA antibodies in IgG and IgA isotypes were determined by ELISA with mannan as a target antigen.


RESULTS
P-ANCA and ASCA were studied in a group of 86 patients (38 CD, 26 UC, 3 non-inflammatory gastrointestinal disorder, 19 health controls). P-ANCA was associated with UC in 46%. ASCA was associated with CD in 76%. Specificity of ANCA for UC compared to healthy controls was 100%, specificity of ASCA for CD compared to healthy controls was 89.5%.


CONCLUSIONS
Although the sensitivity of ASCA and p-ANCA is low, their specificity is high, especially when combining these two markers. We think that combined assay for ASCA and p-ANCA is more useful in IBD.
Malícková
K
K
Ustav klinické imunologie a alergologie VFN a 1. LF UK, Praha. kmali@lf1.cuni.cz
Janatková
I
I
Fucíková
T
T
Adamec
S
S
Lukás
M
M
D019268:Antibodies, Antineutrophil Cytoplasmic / Q000032:analysis; D000908:Antibodies, Fungal / Q000032:analysis*; D015415:Biomarkers / Q000032:analysis; D003093:Colitis, Ulcerative / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology; D003424:Crohn Disease / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology; D003937:Diagnosis, Differential; D006801:Humans; D012441:Saccharomyces cerevisiae / Q000276:immunology*; D012680:Sensitivity and Specificity
D019268:Antibodies, Antineutrophil Cytoplasmic; D000908:Antibodies, Fungal; D015415:Biomarkers