Defining the repeating elements in the cysteine-rich region (CRR) of the CD18 integrin beta 2 subunit.
The cysteine-rich region (CRR) of the integrin beta subunits is organised into four repeating elements. By expression of a panel of truncated beta 2 subunits, and CRR segments fused to the C-terminal end of a CD4 soluble fragment, the segment required for the expression of two monoclonal antibody conformational epitopes was determined. This segment, E482-Q574, contains 16 cysteines representing two repeating units. We have thus defined the CRR unit motif of 'xC---C---C---CxCxxCxC---Cx', where 'x' represents a single residue, and '---' represents a stretch of four to 14 residues.
Tan
S M
SM
Department of Biochemistry, University of Oxford, UK.
Walters
S E
SE
Mathew
E C
EC
Robinson
M K
MK
Drbal
K
K
Shaw
J M
JM
Law
S K
SK
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D018821:CD18 Antigens / Q000235:genetics / Q000276:immunology*; D015704:CD4 Antigens / Q000235:genetics / Q000378:metabolism; D019556:COS Cells; D003545:Cysteine; D000939:Epitopes / Q000737:chemistry*; D008969:Molecular Sequence Data; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology*; D020449:Repetitive Sequences, Amino Acid
D000911:Antibodies, Monoclonal; D018821:CD18 Antigens; D015704:CD4 Antigens; D000939:Epitopes; D011993:Recombinant Fusion Proteins; D003545:Cysteine