Electron-microscopic cytochemical localization of diamine and polyamine oxidases in pea and maize tissues.
An electron-microscopic cytochemical method was used to localize diamine oxidase (DAO) in pea and polyamine oxidase (PAO) in maize (Zea mays L.).  The method, based on the precipitation of amine-oxidase-generated H2O2 by CeCl3, was shown to be specific for DAO and PAO and permitted their localization in plant tissues with a high degree of resolution.  Both enzymes are localized exclusively in the cell wall.  Both DAO- and PAO-activity staining is most intense in the middle lamellar region of the wall and in cells exhibiting highly lignified walls.  The oxidases could provide H2O2 for peroxidase-mediated cross-linking reactions in the cell wall and may, in this capacity, play a role in the regulation of plant growth.
Slocum
R D
RD
Department of Biology, Williams College, Williamstown, MA 01267, USA.
Furey
M J
MJ
D006631:Amine Oxidase (Copper-Containing) / Q000032:analysis* / Q000378:metabolism / Q000502:physiology; D000640:Amitrole; D002473:Cell Wall / Q000201:enzymology* / Q000648:ultrastructure; D002563:Cerium; D004791:Enzyme Inhibitors; D006146:Guanidines; D006651:Histocytochemistry; D006834:Hydrazines; D006861:Hydrogen Peroxide / Q000378:metabolism; D008854:Microscopy, Electron; D016877:Oxidants; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000032:analysis* / Q000378:metabolism / Q000502:physiology; D018532:Pisum sativum / Q000201:enzymology* / Q000648:ultrastructure; D013379:Substrate Specificity; D003313:Zea mays / Q000201:enzymology* / Q000648:ultrastructure; D000097667:Polyamine Oxidase
D006631:Amine Oxidase (Copper-Containing); D000640:Amitrole; D002563:Cerium; D004791:Enzyme Inhibitors; D006146:Guanidines; D006834:Hydrazines; D006861:Hydrogen Peroxide; D016877:Oxidants; D000587:Oxidoreductases Acting on CH-NH Group Donors; D000097667:Polyamine Oxidase; C037834:2-hydroxyethylhydrazine; C004479:pimagedine; C026690:cerous chloride