High-performance liquid chromatography of oligoguanylates at high pH.
Because of the stable self-structures formed by oligomers of guanosine, standard high-performance liquid chromatography techniques for oligonucleotide fractionation are not applicable.  Previously, oligoguanylate separations have been carried out at pH 12 using RPC-5 as the packing material.  While RPC-5 provides excellent separations, there are several limitations, including the lack of a commercially available source.  This report describes a new anion-exchange high-performance liquid chromatography method using HEMA-IEC BIO Q, which successfully separates different forms of the guanosine monomer as well as longer oligoguanylates.  The reproducibility and stability at high pH suggests a versatile role for this material.
Stribling
R
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Department of Zoology, University of California at Davis 95616, USA.
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006151:Guanosine / Q000032:analysis*; D006157:Guanosine Monophosphate / Q000031:analogs & derivatives* / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D009841:Oligonucleotides / Q000032:analysis* / Q000737:chemistry*; D010472:Perchlorates; D011066:Poly C; D011068:Poly G; D012822:Silicon Dioxide; D017670:Sodium Compounds
D009841:Oligonucleotides; D010472:Perchlorates; D017670:Sodium Compounds; D006151:Guanosine; D011068:Poly G; D011066:Poly C; D012822:Silicon Dioxide; C036896:guanosine 5'-phospho-2-methylimidazolide; D006157:Guanosine Monophosphate; C031068:sodium perchlorate