Mitochondrial control of inducible nitric oxide production in stimulated RAW 264.7 macrophages.
Addition of glucose to activated RAW 264.7 macrophages or addition of mitochondrial electron-transfer chain inhibitors enhanced the cellular nitric oxide production. An additive effect of rotenone or antimycin A and glucose on enhancing nitric oxide production was shown. Uncoupling the mitochondria by a chemical uncoupler decreased nitric oxide production. The mitochondria membrane potential was found to be important for cell viability. Although nitric oxide is the physiological inhibitor of mitochondrial respiration, this study indicates that mitochondria were not inhibited in the activated macrophages. Furthermore, a role of mitochondria in the rapid regulation of nitric oxide synthesis by the inducible nitric oxide synthase has been demonstrated.
Tirosh
O
O
Institute of Biochemistry, Food Science and Nutrition, The Hebrew University of Jerusalem, Rehovot, Israel. orentirosh@hotmail.com
Guo
Q
Q
Sen
C K
CK
Packer
L
L
D000818:Animals; D000968:Antimycin A / Q000494:pharmacology; D001120:Arginine / Q000494:pharmacology; D002460:Cell Line; D004357:Drug Synergism; D004578:Electron Spin Resonance Spectroscopy; D004579:Electron Transport / Q000187:drug effects; D042967:Electron Transport Complex I; D014450:Electron Transport Complex III / Q000037:antagonists & inhibitors; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology; D008262:Macrophage Activation; D008264:Macrophages / Q000201:enzymology*; D008564:Membrane Potentials; D051379:Mice; D008928:Mitochondria / Q000187:drug effects / Q000502:physiology*; D009247:NADH, NADPH Oxidoreductases / Q000037:antagonists & inhibitors; D009249:NADP / Q000378:metabolism; D009569:Nitric Oxide / Q000096:biosynthesis*; D019001:Nitric Oxide Synthase / Q000096:biosynthesis* / Q000235:genetics; D052247:Nitric Oxide Synthase Type II; D012402:Rotenone / Q000494:pharmacology; D019097:Spin Trapping; D013482:Superoxide Dismutase / Q000494:pharmacology; D019323:omega-N-Methylarginine / Q000494:pharmacology
D004791:Enzyme Inhibitors; D012402:Rotenone; D019323:omega-N-Methylarginine; D009569:Nitric Oxide; D009249:NADP; D000968:Antimycin A; D001120:Arginine; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse; D013482:Superoxide Dismutase; D009247:NADH, NADPH Oxidoreductases; D042967:Electron Transport Complex I; D014450:Electron Transport Complex III; D005947:Glucose