Microscale determination of the spectral characteristics and carbon-isotopic compositions of porphyrins.
Molar extinction coefficients for band III of Ni porphyrins are calculated from results of spectrophotometric and manometric analyses of individual etioporphyrins, DPEP, cyclic, and diDPEP porphyrins known to initially be pure from mass spectrometry, 1H NMR, and analytical HPLC studies.  A method for determining carbon-isotopic compositions and purity of micromolar quantities of individual porphyrins using combined spectrophotometric and manometric techniques is presented.
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Biogeochemical Laboratories, Indiana University, Bloomington 47405-5101.
Hayes
J M
JM
Boreham
C J
CJ
D002244:Carbon / Q000032:analysis*; D002247:Carbon Isotopes; D002851:Chromatography, High Pressure Liquid; D055593:Geological Phenomena; D005844:Geology; D009682:Magnetic Resonance Spectroscopy; D008365:Manometry; D013058:Mass Spectrometry; D009532:Nickel; D011166:Porphyrins / Q000032:analysis* / Q000737:chemistry* / Q000302:isolation & purification; D013053:Spectrophotometry
D002247:Carbon Isotopes; D011166:Porphyrins; D002244:Carbon; D009532:Nickel