Sequence analysis of env C2/V3, gag p17/p24, and pol protease regions of 25 HIV type 1 isolates from Ho Chi Minh City, Vietnam.
Env C2/V3, gag p17/p24, pol protease, and RT regions of HIV-1 isolates recently obtained from 25 HIV-1 seropositive individuals from Ho Chi Minh City (Vietnam) were studied, and genes subtypes were determined by DNA sequence analyses. Twenty-three isolates out of 25 were identified as belonging to subtype E, now recognized as circulating recombinant form 1 (CRF01_AE). The motif at the top of the V3 loop (generally GPGQ) was then preceded by an isoleucine or a methionine (M) residue; the M residue might be a local signature of Vietnamese E isolates compared to Thai E viruses. Two isolates (8%) were shown to be intersubtype recombinants: one E/B and one CRF02_AG(IBNG)/D. The polymorphism of pol protease was considered only for CRF01_AE isolates and is clearly different from that recorded for B viruses with substitutions at positions 13, 35, 36, 41, 69, and 89.
Caumont
A
A
Laboratoire de Virologie, Université Victor Ségalen Bordeaux 2, Bordeaux, France.
Lan
N T
NT
Uyen
N T
NT
Hung
P V
PV
Schvoerer
E
E
Urriza
M S
MS
Roques
P
P
Schrive
M H
MH
Lien
T T
TT
Lafon
M E
ME
Dormont
D
D
Barre-Sinoussi
F
F
Fleury
H J
HJ
D000293:Adolescent; D000328:Adult; D000595:Amino Acid Sequence; D005260:Female; D015686:Gene Products, env / Q000737:chemistry / Q000235:genetics; D015752:Genes, env / Q000235:genetics*; D015750:Genes, gag / Q000235:genetics*; D015751:Genes, pol / Q000235:genetics*; D015658:HIV Infections / Q000453:epidemiology* / Q000821:virology*; D015497:HIV-1 / Q000737:chemistry / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D011110:Polymorphism, Genetic / Q000235:genetics; D016415:Sequence Alignment; D014744:Vietnam / Q000453:epidemiology
D015686:Gene Products, env