Novel nucleoside triphosphate analogs for the enzymatic labeling of nucleic acids.
We have evaluated several novel nucleotide analogs suitable for enzymatic labeling of nucleic acid targets for a variety of array-based assays. Two new reagents in particular, a C4-labeled 1-(2',3'-dideoxy-beta-D-ribofuranosyl) imidazole-4-carboxamide 5'-triphosphate 5 and an N1-labeled 5-(beta-D-ribofuranosyl)-2,4(1H,3H)-pyrimidinedione 5'-triphosphate 3, were found to be excellent substrates for labeling with terminal deoxynucleotidyl transferase and T7 RNA polymerase, respectively.
Barone
A D
AD
Affymetrix, Inc., Santa Clara, California, USA.
Chen
C
C
McGall
G H
GH
Rafii
K
K
Buzby
P R
PR
Dimeo
J J
JJ
D001710:Biotin / Q000031:analogs & derivatives; D004253:DNA Nucleotidylexotransferase / Q000737:chemistry*; D015342:DNA Probes / Q000737:chemistry*; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry*; D005452:Fluoresceins / Q000737:chemistry; D009693:Nucleic Acid Hybridization; D009711:Nucleotides / Q000737:chemistry*; D014764:Viral Proteins
D015342:DNA Probes; D005452:Fluoresceins; D009711:Nucleotides; D014764:Viral Proteins; D001710:Biotin; C062210:bacteriophage T7 RNA polymerase; D004253:DNA Nucleotidylexotransferase; D012321:DNA-Directed RNA Polymerases