Acceleration of nucleic acid hybridization on DNA microarrays driven by pH tunable modifications.
A series of peptides containing histidine residues were designed as potential hybridization rate enhancers within a polymeric matrix of DNA microarrays. The polymeric matrix modified with these peptides showed strong attraction to DNA molecules under conditions of induction. DNA probes on the peptide-modified sites rapidly hybridized to their complementary targets with single base pair mismatch discrimination.
Zhang
P
P
Motorola BioChip Systems, Tempe, Arizona, USA.
Briones
N
N
Liu
C G
CG
Brush
C K
CK
Powdrill
T
T
Belosludtsev
Y
Y
Hogan
M
M
D004247:DNA / Q000235:genetics / Q000378:metabolism; D015342:DNA Probes; D015870:Gene Expression; D006639:Histidine / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D009693:Nucleic Acid Hybridization / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis; D010455:Peptides / Q000737:chemistry*; D013379:Substrate Specificity
D015342:DNA Probes; D010455:Peptides; D006639:Histidine; D004247:DNA