Neoplastic transformation of mouse C3H 10T1/2 and Syrian hamster embryo cells by heavy ions.
C3H 10T1/2 mouse-embryo fibroblasts were used for transformation experiments to study the effectiveness of various heavy ions with energies up to 20 MeV/u and LET values from 170 to 16,000 keV/micrometers. The transformation frequency per unit absorbed dose decreased with increasing ionization density; at the highest values of LET we found a decrease even of the transformation efficiency per unit fluence. Uranium ions at energies of 5, 9, and 16.3 MeV/u did not induce any transformation. In additional studies primary Syrian hamster embryo cells (SHE) were exposed to heavy ions in order to characterize cytological and molecular changes which may be correlated with neoplastic transformation. Growth behaviour, chromosomal status, tumorigenicity in nude mice, and expression of oncogenes of transformed cell lines were examined
Hieber
L
L
Institut fur Medizinische Strahlenkunde der Universitat Wurzburg, F.R.G.
Ponsel
G
G
Trutschler
K
K
Fenn
S
S
Kellerer
A M
AM
D000818:Animals; D002455:Cell Division; D002461:Cell Line, Transformed; D002470:Cell Survival; D002471:Cell Transformation, Neoplastic; D002875:Chromosomes; D006224:Cricetinae; D004307:Dose-Response Relationship, Radiation; D004622:Embryo, Mammalian / Q000166:cytology; D005347:Fibroblasts; D015972:Gene Expression Regulation, Neoplastic; D011905:Genes, ras; D020450:Heavy Ions; D018499:Linear Energy Transfer; D008647:Mesocricetus; D051379:Mice; D008809:Mice, Inbred C3H; D012062:Relative Biological Effectiveness; D014407:Tumor Cells, Cultured