title: The influence of radiation quality on the formation of DNA breaks.
abstract: We have aimed to present a comprehensive review of our understanding to date of the formation of DNA strand breaks induced by high LET radiation. We have discussed data obtained from DNA in solution as well as from the formation and "repair" of strand breaks in cell DNA. There is good agreement, qualitatively, between these two systems. Results were evaluated for two parameters: (1) effectivity per particle, the cross section (sigma) in micrometers 2/particle;  and (2) the strand break induction frequency as number of breaks per Gy per unit DNA (bp or dalton). A series of biological effects curves (one for each Z-number) is obtained in effectivity versus LET plots. The relationships between induction frequencies of single-strand breaks, or double-strand breaks, or the residual "irrepairable" breaks and LET-values have been evaluated and discussed for a wide spectrum of heavy ions, both for DNA in solution and for DNA in the cell. For radiation induced total breaks in cell DNA, the RBE is less than one, while the RBE for the induction of DSBs can be greater than one in the 100-200 keV/micrometers range. The level of irrepairable strand breaks is highest in this same LET range and may reach 25 percent of the initial break yield. The data presented cover results obtained for helium to uranium particles, covering a particle incident energy range of about 2 to 900 MeV/u with a corresponding LET range of near 16 to 16000 keV/micrometers.
authors.affiliation: Cell and Molecular Biology Division, Lawrence Berkeley Laboratory, University of California, Berkeley 94720, USA.
authors.forename: R
authors.initials: R
authors.lastname: Roots
authors.forename: W
authors.initials: W
authors.lastname: Holley
authors.forename: A
authors.initials: A
authors.lastname: Chatterjee
authors.forename: E
authors.initials: E
authors.lastname: Rachal
authors.forename: G
authors.initials: G
authors.lastname: Kraft
mesh_terms: D000818:Animals; D002478:Cells, Cultured / Q000528:radiation effects; D006224:Cricetinae; D003412:Cricetulus; D004249:DNA Damage; D004260:DNA Repair; D004271:DNA, Fungal; D004279:DNA, Viral / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007668:Kidney / Q000166:cytology; D018499:Linear Energy Transfer; D010315:Particle Accelerators; D012062:Relative Biological Effectiveness; D013539:Simian virus 40 / Q000235:genetics / Q000528:radiation effects*
chemical_list: D004271:DNA, Fungal; D004279:DNA, Viral