Calcium signals in rat basophilic leukemia (RBL-2H3) cells primed with the neuropeptide substance P.
Communication between nerves and mast cells is a prototypic demonstration of neuroimmune interaction. We have recently shown that direct nerve-mast cell cross-talk can occur in the absence of an intermediary transducing cell and that the neuropeptide substance P is an important mediator of this communication. Here we study the calcium signals in rat basophilic leukemia cells (RBL-2H3; mucosal-type mast cells) primed with substance P. RBL cells responded only slightly to stimulation with compound 48/80, however they responded to the stimulation when the cells had been primed with substance P (0.5 microM) for one week. The present results provide a foundation to study the neuroimmune cross-talk in a co-culture system.
Furukawa
Y
Y
Faculty of Pharmaceutical Sciences, Nagoya City University, Nagoya, Japan.
Furuno
T
T
Teshima
R
R
Nakanishi
M
M
D000818:Animals; D020013:Calcium Signaling / Q000187:drug effects*; D005456:Fluorescent Dyes; D016257:Fura-2; D006636:Histamine Release / Q000187:drug effects; D007700:Kinetics; D015471:Leukemia, Basophilic, Acute / Q000378:metabolism*; D008407:Mast Cells / Q000378:metabolism*; D051381:Rats; D020239:Receptor Cross-Talk / Q000187:drug effects; D013050:Spectrometry, Fluorescence; D013268:Stimulation, Chemical; D013373:Substance P / Q000378:metabolism / Q000494:pharmacology*; D014407:Tumor Cells, Cultured; D001619:beta-N-Acetylhexosaminidases / Q000378:metabolism; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
D005456:Fluorescent Dyes; D013373:Substance P; D003189:p-Methoxy-N-methylphenethylamine; D001619:beta-N-Acetylhexosaminidases; D016257:Fura-2