Determination of L-ascorbic acid in Lycopersicon fruits by capillary zone electrophoresis.
This study shows an improved method for the determination of L-ascorbic acid (l-AA) in fruits of Lycopersicon by capillary zone electrophoresis (CZE). Two backgrounds electrolytes (BGEs) have been tested: (i) 400 mM borate at pH 8.0 and 1 x 10(-2)% hexadimethrine bromide, for the separation of Eulycopersicon subgenus species; and (ii) as in BGE(i) but supplemented with 20% (v/v) acetonitrile, for the separation of species of the Eriopersicon subgenus. The present procedures were compared with two routine methods-enzymatic assay and potentiometric titration with 2,6-dichlorophenol-indophenol. While these routine methods presented some difficulties in quantifying l-AA in several Lycopersicon fruits, CZE was successfully applied in all the analyzed samples. The proposed CZE protocols give lower detection limits (<0.4 microg ml(-1)); are cheaper, quicker, and highly reproducible; and can be applied to analyze large series of samples (ca. 50 samples per day) which is utmost importance, not only in screening trials for internal quality and tomato breeding programs, but also in systematic and routine characterization of Lycopersicon fruits.
Galiana-Balaguer
L
L
Department of Biotechnology, Genetics, Polytechnic University of Valencia, Valencia, E-46022, Spain.
Roselló
S
S
Herrero-Martínez
J M
JM
Maquieira
A
A
Nuez
F
F
D001205:Ascorbic Acid / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods*; D005638:Fruit / Q000737:chemistry; D018551:Solanum lycopersicum / Q000737:chemistry*; D011199:Potentiometry / Q000379:methods
D001205:Ascorbic Acid