African swine fever virus protein p54 interacts with the microtubular motor complex through direct binding to light-chain dynein.
Dynein is a minus-end-directed microtubule-associated motor protein involved in cargo transport in the cytoplasm. African swine fever virus (ASFV), a large DNA virus, hijacks the microtubule motor complex cellular transport machinery during virus infection of the cell through direct binding of virus protein p54 to the light chain of cytoplasmic dynein (LC8). Interaction of p54 and LC8 occurs both in vitro and in cells, and the two proteins colocalize at the microtubular organizing center during viral infection. p50/dynamitin, a dominant-negative inhibitor of dynein-dynactin function, impeded ASFV infection, suggesting an essential role for dynein during virus infection. A 13-amino-acid domain of p54 was sufficient for binding to LC8, an SQT motif within this domain being critical for this binding. Direct binding of a viral structural protein to LC8, a small molecule of the dynein motor complex, could constitute a molecular mechanism for microtubule-mediated virus transport.
Alonso
C
C
Departamento de Biotecnología, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Universidad Complutense, Madrid, Spain. calonso@inia.es
Miskin
J
J
Hernáez
B
B
Fernandez-Zapatero
P
P
Soto
L
L
Cantó
C
C
Rodríguez-Crespo
I
I
Dixon
L
L
Escribano
J M
JM
D000358:African Swine Fever Virus / Q000502:physiology; D000595:Amino Acid Sequence; D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D002522:Chlorocebus aethiops; D029721:Drosophila Proteins; D000072159:Dynactin Complex; D004398:Dyneins / Q000494:pharmacology; D008869:Microtubule-Associated Proteins / Q000494:pharmacology; D022101:Microtubule-Organizing Center / Q000378:metabolism*; D011485:Protein Binding; D017027:Protein Tyrosine Phosphatases / Q000037:antagonists & inhibitors; D014638:Vanadates / Q000494:pharmacology; D014709:Vero Cells; D015678:Viral Structural Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014779:Virus Replication / Q000187:drug effects
D002352:Carrier Proteins; D029721:Drosophila Proteins; D000072159:Dynactin Complex; D008869:Microtubule-Associated Proteins; D015678:Viral Structural Proteins; C093921:p54 protein, African swine fever virus; D014638:Vanadates; D017027:Protein Tyrosine Phosphatases; D004398:Dyneins