title: Polyimidazole conjugated oligonucleotides reach the nucleus of HeLa cells.
abstract: Oligonucleotide models bearing 6, 12 or 18 histamine residues were synthesized on solid support and labeled with fluorescein. Only the oligo with 6 histamine residues showed a high uptake in HeLa cells with a nuclear localization. Experiment a 4 degrees C or with bafilomicyn A1 suggest that uptake proceeded by an endocytosis mechanism followed by a destabilization of the membrane. Once in the cytoplasm the oligo reached rapidly the nucleus.
authors.affiliation: Laboratoire de Chimie Organique Biomoléculaire de Synthèse, UMR 5625 CNRS-UM II, Université Montpellier II, Place E. Bataillon, 34095 Montpellier, France.
authors.forename: F
authors.initials: F
authors.lastname: Morvan
authors.forename: C
authors.initials: C
authors.lastname: Castex
authors.forename: E
authors.initials: E
authors.lastname: Vivès
authors.forename: J L
authors.initials: JL
authors.lastname: Imbach
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002467:Cell Nucleus / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D019793:Fluorescein / Q000737:chemistry; D006367:HeLa Cells; D006632:Histamine / Q000031:analogs & derivatives / Q000493:pharmacokinetics; D006801:Humans; D007093:Imidazoles / Q000737:chemistry / Q000493:pharmacokinetics*; D018942:Macrolides; D008856:Microscopy, Fluorescence; D009841:Oligonucleotides / Q000737:chemistry / Q000493:pharmacokinetics*; D063065:Organophosphonates / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013936:Thymidine / Q000031:analogs & derivatives / Q000493:pharmacokinetics
chemical_list: D000900:Anti-Bacterial Agents; D004791:Enzyme Inhibitors; D007093:Imidazoles; D018942:Macrolides; D009841:Oligonucleotides; D063065:Organophosphonates; C057620:bafilomycin A; D006632:Histamine; D019793:Fluorescein; D013936:Thymidine