Using electron-microscopy images as a model for molecular replacement.
This review addresses the technical problems encountered while using models based on electron microscopy to generate initial phases for crystallographic studies. The test cases used were the gp6 portal protein with 13-fold rotational symmetry and the human hepatitis virus HepB, a viral assembly with T = 4 icosohedral symmetry.
Dodson
E J
EJ
York Structural Biology Laboratory, Chemistry Department, University of York, York YO10 5DD, England. e.dodson@ysbl.york.ac.uk
D002213:Capsid / Q000737:chemistry; D003461:Crystallography; D006515:Hepatitis B virus / Q000737:chemistry; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008854:Microscopy, Electron; D008958:Models, Molecular; D054334:Viral Regulatory and Accessory Proteins / Q000737:chemistry
D054334:Viral Regulatory and Accessory Proteins; C073671:SPP1 pac region protein, Bacteriophage SPP1