Assessing the impact of Cry1Ab-expressing corn pollen on monarch butterfly larvae in field studies.
Survival and growth of monarch larvae, Danaus plexippus (L.), after exposure to either Cry1Ab-expressing pollen from three Bacillus thuringiensis (Bt) corn (Zea mays L.) events differing in toxin expression or to the insecticide, lambda-cyhalothrin, were examined in field studies. First instars exposed to low doses ( approximately 22 grains per cm(2)) of event-176 pollen gained 18% less weight than those exposed to Bt11 or Mon810 pollen after a 5-day exposure period. Larvae exposed to 67 pollen grains per cm(2) on milkweed leaves from within an event-176 field exhibited 60% lower survivorship and 42% less weight gain compared with those exposed to leaves from outside the field. In contrast, Bt11 pollen had no effect on growth to adulthood or survival of first or third instars exposed for 5 days to approximately 55 and 97 pollen grains per cm(2), respectively. Similarly, no differences in larval survivorship were observed after a 4-day exposure period to leaves with 504-586 (within fields) or 18-22 (outside the field) pollen grains per cm(2) collected from Bt11 and non-Bt sweet-corn fields. However, survivorship and weight gain were drastically reduced in non-Bt fields treated with lambda-cyhalothrin. The effects of Bt11 and Mon810 pollen on the survivorship of larvae feeding 14 to 22 days on milkweeds in fields were negligible. Further studies should examine the lifetime and reproductive impact of Bt11 and Mon810 pollen on monarchs after long-term exposure to naturally deposited pollen.
Stanley-Horn
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Department of Environmental Biology, University of Guelph, Guelph, ON, Canada. destanle@uoguelph.ca
Dively
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Hellmich
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Mattila
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Sears
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MK
Rose
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Jesse
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Losey
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Obrycki
J J
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Lewis
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D000818:Animals; D001413:Bacillus thuringiensis; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000009:adverse effects* / Q000235:genetics; D001427:Bacterial Toxins; D002080:Butterflies; D004731:Endotoxins / Q000009:adverse effects* / Q000235:genetics; D015870:Gene Expression; D006460:Hemolysin Proteins; D007484:Iowa; D007814:Larva; D008396:Maryland; D009518:New York; D009864:Ontario; D010572:Pest Control, Biological / Q000379:methods; D030821:Plants, Genetically Modified; D011058:Pollen; D003313:Zea mays
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis