[Effect of age of cell donors of nuclei on effectivness of developing cloned rabbit embryos].
We studied the capacity of nuclei of rabbit fibroblasts taken from various developmental stages for reprogramming in the cytoplasm of mature aging enucleated oocytes and development of the cloned embryos to the preimplantation stages. A negative correlation was found between the age of an animal--donor of fibroblasts and efficiency of the development of cloned embryos (rmorula-blastocyst = -0.826, rblastocyst = -0.7139). A reliably decreased capacity for reprogramming of the nuclei of donor fibroblasts was shown upon transition from prenatal development to the postnatal one, as well as a trend to a decreased capacity of nuclei for reprogramming during aging. Aging of cells in the culture, at least until the 10th passage, did not affect the capacity of the nuclei of fetal fibroblasts for reprogramming and development of cloned embryos.
Lagutina
I S
IS
Research and Production Biotechnological Center for Animal Husbandry, Russian Academy of Agricultural Sciences, Gorki Leninskie, Moscow Oblast, 142712 Russia.
Mezina
M N
MN
Prokof'ev
M I
MI
Chernykh
V Ia
VIa
Galat
V V
VV
D000367:Age Factors; D000818:Animals; D002478:Cells, Cultured; D016922:Cellular Senescence; D019976:Cloning, Organism; D005314:Embryonic and Fetal Development; D005260:Female; D053652:Nuclear Transfer Techniques; D011817:Rabbits