title: Identification and functional reconstitution of the yeast peroxisomal adenine nucleotide transporter.
abstract: The requirement for small molecule transport systems across the peroxisomal membrane has previously been postulated, but not directly proven. Here we report the identification and functional reconstitution of Ant1p (Ypr128cp), a peroxisomal transporter in the yeast Saccharomyces cerevisiae, which has the characteristic sequence features of the mitochondrial carrier family. Ant1p was found to be an integral protein of the peroxisomal membrane and expression of ANT1 was oleic acid inducible. Targeting of Ant1p to peroxisomes was dependent on Pex3p and Pex19p, two peroxins specifically required for peroxisomal membrane protein insertion. Ant1p was essential for growth on medium-chain fatty acids as the sole carbon source. Upon reconstitution of the overexpressed and purified protein into liposomes, specific transport of adenine nucleotides could be demonstrated. Remarkably, both the substrate and inhibitor specificity differed from those of the mitochondrial ADP/ATP transporter. The physiological role of Ant1p in S.cerevisiae is probably to transport cytoplasmic ATP into the peroxisomal lumen in exchange for AMP generated in the activation of fatty acids.
authors.affiliation: Department of Pharmaco-Biology, Laboratory of Biochemistry and Molecular Biology, University of Bari, Via E. Orabona 4, 70125 Bari, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Palmieri
authors.forename: H
authors.initials: H
authors.lastname: Rottensteiner
authors.forename: W
authors.initials: W
authors.lastname: Girzalsky
authors.forename: P
authors.initials: P
authors.lastname: Scarcia
authors.forename: F
authors.initials: F
authors.lastname: Palmieri
authors.forename: R
authors.initials: R
authors.lastname: Erdmann
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D001692:Biological Transport; D002455:Cell Division; D005656:Fungal Proteins / Q000235:genetics / Q000502:physiology*; D007425:Intracellular Membranes / Q000378:metabolism; D000226:Mitochondrial ADP, ATP Translocases / Q000235:genetics / Q000502:physiology*; D008954:Models, Biological; D019301:Oleic Acid / Q000378:metabolism; D020675:Peroxisomes / Q000378:metabolism*; D011510:Proteolipids / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000378:metabolism*; D014162:Transfection
chemical_list: D005656:Fungal Proteins; D011510:Proteolipids; C020485:proteoliposomes; D019301:Oleic Acid; D000255:Adenosine Triphosphate; D000226:Mitochondrial ADP, ATP Translocases