Plant reproduction in spaceflight environments.
Because plant reproduction is a complex developmental process there are many possible sites of perturbation by the unusual environments of orbital spacecraft. Previous long-duration experiments on Soviet platforms shared features of slowed development through the vegetative stage of plant growth and aborted reproductive function. Our goal has been to understand how special features of the spaceflight environment impact physiological function and reproductive development. In a series of short-duration experiments in the Shuttle mid-deck we studied early reproductive development in Arabidopsis thaliana. Pollen and ovule development aborted at an early stage in the first experiment on STS-54 which utilized closed plant growth chambers. Post-flight analysis suggested that the plants may have been carbon dioxide limited. Subsequent experiments utilized carbon dioxide enrichment (on STS-51) and cabin air flow-through with an air exchange system (on STS-68). Both modifications allowed pollen and ovule development to occur normally on orbit, and full reproductive development up to the stage of an immature seed occurred on STS-68. However, analysis of plant roots from these experiments demonstrated a limitation in rootzone aeration in the spaceflight material that was not mitigated by these procedures. In the future, additional resources (crew time, upgraded flight hardware, and special platforms) will invite more elaborate, long-duration experimentation. On the ISS, a variable speed centrifuge and upgraded plant habitats will permit detailed experiments on the role of gravity in shaping the plant micro-environment, and what influence this plays during reproduction.
Musgrave
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ME
Department of Plant Pathology, Louisiana State University Agricultural Center, Baton Rouge 70803, USA.
Kuang
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A
Porterfield
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D017360:Arabidopsis / Q000196:embryology / Q000254:growth & development* / Q000378:metabolism*; D002245:Carbon Dioxide / Q000378:metabolism; D018504:Ecological Systems, Closed; D008021:Life Support Systems; D010100:Oxygen / Q000378:metabolism; D063245:Plant Development; D010944:Plants / Q000196:embryology / Q000378:metabolism; D012098:Reproduction; D012639:Seeds / Q000254:growth & development; D013026:Space Flight; D014893:Weightlessness
D002245:Carbon Dioxide; D010100:Oxygen