Detection and rapid increase of salivary antibodies to Staphylococcus lentus, an indigenous bacterium in rabbit saliva, through a single tonsillar application of bacterial cells.
In rabbits, Staphylococcus lentus is one of the major bacteria in saliva and a minor bacteria in jejunum fluids and nasal wash. The presence and induction of naturally occurring antibodies reacting to rabbit indigenous bacteria were studied. In non-immune rabbits, the proportion of anti-S. lentus IgA antibodies to total IgA in the saliva was several times higher than that in the intestinal fluids and the nasal wash. The salivary antibodies were found to have increased 1 week after a single tonsillar application of isolated S. lentus cells but not after a single nasal application or a single intragastric instillation. In addition, the anti-S. lentus antibodies in the saliva highly increased with weekly tonsillar applications of isolated S. lentus but increased only one-fifth with weekly nasal applications of the same cells. These results strongly suggest that the palatine tonsils, which we believe had already been sensitized by S. lentus in the physiological condition, induced naturally occurring antibodies in rabbit saliva.
Kanda
M
M
First Department of Oral and Maxillofacial Surgery, Kyushu Dental College, Kitakyushu, Fukuoka, Japan.
Inoue
H
H
Fukuizumi
T
T
Tsujisawa
T
T
Tominaga
K
K
Fukuda
J
J
D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis* / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay; D007110:Immunity, Active; D018928:Immunity, Mucosal; D007132:Immunoglobulin Isotypes; D008297:Male; D014066:Palatine Tonsil / Q000276:immunology / Q000382:microbiology*; D011817:Rabbits / Q000276:immunology*; D012463:Saliva / Q000276:immunology* / Q000382:microbiology; D013203:Staphylococcal Infections / Q000276:immunology; D013210:Staphylococcus / Q000276:immunology*; D018709:Statistics, Nonparametric
D000907:Antibodies, Bacterial; D007132:Immunoglobulin Isotypes