Protein kinase D interacts with Golgi via its cysteine-rich domain.
Protein kinase D (PKD)/protein kinase Cmu is a serine/threonine protein kinase that has been localized in the cytosol and in several intracellular compartments including Golgi, mitochondria and plasma membrane. Using real time imaging of fluorescent protein (GFP)-tagged PKD, we have found that the accumulation of PKD in the Golgi compartment, following a temperature shift from 37 to 20 degrees C, was mediated by the cysteine-rich domain (CRD) of PKD. The CRD of PKD also mediates its interaction with the plasma membrane, further supporting the conclusion that the CRD of PKD may act as a subcellular localization signal.
Rey
O
O
Unit of Signal Transduction and Gastrointestinal Cancer, Division of Digestive Diseases, UCLA School of Medicine, Los Angeles, CA 90095-1786, USA.
Rozengurt
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E
D016475:3T3 Cells; D000818:Animals; D002384:Catalysis; D003545:Cysteine / Q000378:metabolism*; D006056:Golgi Apparatus / Q000378:metabolism*; D051379:Mice; D011493:Protein Kinase C / Q000737:chemistry / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D013347:Subcellular Fractions
C089212:protein kinase D; D011493:Protein Kinase C; D003545:Cysteine