title: Distinct regulatory elements are required for faithful expression of human CD4 in T cells, macrophages, and dendritic cells of transgenic mice.
abstract: To identify the regulatory elements controlling expression of the human CD4 (hCD4) gene in different cell types of the immune system, deletion and chimeric (human/murine) reporter genes were constructed and tested in transgenic (Tg) mice. Regulatory elements required for the proper hCD4 expression in the immature double-positive thymic T cells were identified in the enhancer and in the 3' end of intron 1. Expression of hCD4 in macrophages is controlled by at least 2 sets of regulatory elements: one present in front of exon 1 and the second at the 5' end of intron 1. The hCD4 elements required for expression on both myeloid and lymphoid CD8alpha(+) dendritic cells (DCs) from lymph node and thymus were found to be different from those required for macrophage expression. The results indicate that expression of hCD4 in T cells, macrophages, and DCs is controlled by distinct regulatory elements.
authors.affiliation: Laboratory of Molecular Biology, Clinical Research Institute of Montreal, Montreal, Quebec, Canada. hannaz@ircm.qc.ca
authors.forename: Z
authors.initials: Z
authors.lastname: Hanna
authors.forename: N
authors.initials: N
authors.lastname: Rebai
authors.forename: J
authors.initials: J
authors.lastname: Poudrier
authors.forename: P
authors.initials: P
authors.lastname: Jolicoeur
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D015704:CD4 Antigens / Q000235:genetics* / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D003713:Dendritic Cells / Q000378:metabolism*; D005434:Flow Cytometry; D005786:Gene Expression Regulation / Q000235:genetics; D005809:Genes, Regulator / Q000235:genetics*; D006801:Humans; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic; D013601:T-Lymphocytes / Q000378:metabolism*; D014018:Tissue Distribution
chemical_list: D015704:CD4 Antigens; D004247:DNA