Biological potential of extraterrestrial materials. 2. Microbial and plant responses to nutrients in the Murchison carbonaceous meteorite.
Meteoritic materials are investigated as potential early planetary nutrients. Aqueous extracts of the Murchison C2 carbonaceous meteorite are utilized as a sole carbon source by microorganisms, as demonstrated by the genetically modified Pseudomonas fluorescence equipped with the lux gene. Nutrient effects are observed also with the soil microorganisms Nocardia asteroides and Arthrobacter pascens that reach populations up to 5 x 10(7) CFU/ml in meteorite extracts, similar to populations in terrestrial soil extracts. Plant tissue cultures of Asparagus officinalis and Solanum tuberosum (potato) exhibit enhanced pigmentation and some enhanced growth when meteorite extracts are added to partial nutrient media, but inhibited growth when added to full nutrient solution. The meteorite extracts lead to large increases in S, Ca, Mg, and Fe plant tissue contents as shown by X-ray fluorescence, while P, K, and Cl contents show mixed effects. In both microbiological and plant tissue experiments, the nutrient and inhibitory effects appear to be best balanced for growth at about 1:20 (extracted solid : H2O) ratios. The results suggest that solutions in cavities in meteorites can provide efficient concentrated biogenic and early nutrient environments, including high phosphate levels, which may be the limiting nutrient. The results also suggest that carbonaceous asteroid resources can sustain soil microbial activity and provide essential macronutrients for future space-based ecosystems.
Mautner
M N
MN
Space Bioresources Laboratory, Department of Soil Science, Lincoln University, New Zealand. mautnerm@lincoln.ac.nz
Conner
A J
AJ
Killham
K
K
Deamer
D W
DW
D001173:Arthrobacter / Q000254:growth & development / Q000378:metabolism; D002244:Carbon / Q000378:metabolism*; D015169:Colony Count, Microbial; D003470:Culture Media; D046508:Culture Techniques; D005308:Fertilizers; D015964:Gene Expression Regulation, Bacterial; D019668:Liliaceae / Q000254:growth & development* / Q000378:metabolism; D019110:Meteoroids; D009616:Nocardia asteroides / Q000254:growth & development / Q000378:metabolism; D009753:Nutritive Value; D010710:Phosphates / Q000378:metabolism; D011551:Pseudomonas fluorescens / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D012988:Soil Microbiology; D011198:Solanum tuberosum / Q000254:growth & development* / Q000378:metabolism; D014733:Vibrio / Q000235:genetics
D003470:Culture Media; D005308:Fertilizers; D010710:Phosphates; D002244:Carbon