title: Heme oxygenase-2 interaction with metalloporphyrins: function of heme regulatory motifs.
abstract: Heme oxygenase-2 (HO-2) degrades heme [Fe-protoporphyrin IX (Fe-PP)] to CO and bilirubin. The enzyme is a hemoprotein and interacts with nitric oxide. HO-2 has two copies of heme regulatory motif (HRM) with a conserved core of Cys264-Pro265 and Cys281-Pro282. We examined interaction of HO-2 HRMs with Fe-PP, Zn-protoporphyrin IX (Zn-PP; HO-2 inhibitor), and protoporphyrin IX (PP IX). Spectral analyses, using 1:4 or 1:1 molar ratio of the heme to 10-residue peptides, corresponding to HRM containing HO-2 sequences, revealed specific interactions as indicated by a shift in the absorption spectrum of heme. Five residue peptides qualitatively produced similar results. Substitution of cysteine with alanine in either peptide eliminated interactions, and substitution of proline with alanine reduced the peptides' affinity for heme. Neither Zn-PP nor PP IX absorption spectrum was affected by HRM peptides. The circular dichroism spectra confirmed heme-HRM peptides interactions. An astounding 4,000-6,000-fold higher concentrations of KCN were required at pH 7.5 to displace HRM peptides from heme. Data suggest (a) each HRM can contribute to HO-2-heme interaction, (b) heme iron interacts with cysteine thiol, (c) charged residues upstream of Cys264-Pro265 result in its high-affinity heme binding, and (d) inhibition of HO-2 activity by synthetic metalloporphyrins does not involve HRMs. We suggest that heme bound to HRMs may serve as a binding site/reservoir for gaseous signal molecules.
authors.affiliation: Department of Biochemistry and Biophysics, University of Rochester School of Medicine, NY 14642, USA.
authors.forename: T J
authors.initials: TJ
authors.lastname: Huang
authors.forename: W K
authors.initials: WK
authors.lastname: McCoubrey
authors.forename: M D
authors.initials: MD
authors.lastname: Maines
mesh_terms: D020816:Amino Acid Motifs; D019943:Amino Acid Substitution; D020134:Catalytic Domain; D002942:Circular Dichroism; D003545:Cysteine / Q000737:chemistry; D006418:Heme / Q000737:chemistry; D006419:Heme Oxygenase (Decyclizing) / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008665:Metalloporphyrins / Q000378:metabolism*; D010446:Peptide Fragments / Q000737:chemistry; D011392:Proline / Q000737:chemistry; D011485:Protein Binding; D011524:Protoporphyrins / Q000378:metabolism; D013053:Spectrophotometry; D013056:Spectrophotometry, Ultraviolet; D013439:Sulfhydryl Reagents / Q000737:chemistry
chemical_list: D008665:Metalloporphyrins; D010446:Peptide Fragments; D011524:Protoporphyrins; D013439:Sulfhydryl Reagents; C448299:iron protoporphyrin IX; C017803:zinc protoporphyrin; D006418:Heme; D011392:Proline; C028025:protoporphyrin IX; D006419:Heme Oxygenase (Decyclizing); C105245:heme oxygenase-2; D003545:Cysteine