[Study of protective role of the polysaccharide-containing components of capsules of Azospirillum brasilense].
The involvement of the carbohydrate components of the Azospirillum brasilense Sp245 capsules in bacterial protection from the action of extreme factors was investigated. The survival of encapsulated and non-encapsulated azospirilla exposed to elevated (46-48 degrees C) and below-freezing (-20 and -70 degrees C) temperatures, extreme pH values (2 and 10), and to drying was studied. High-molecular-weight carbohydrate-containing complexes (lipopolysaccharide-protein complex and polysaccharide-lipid complex) were isolated from the capsular material of azospirilla. It was shown that the addition of these complexes to the suspension of decapsulated cells before exposing them to extreme factors enhanced their survival rates by 15 to 51%.
Konnova
S A
SA
Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, pr. Entuziastov 13, Saratov, 410015 Russia.
Brykova
O S
OS
Sachkova
O A
OA
Egorenkova
I V
IV
Ignatov
V V
VV
D000220:Adaptation, Biological; D015806:Azospirillum brasilense / Q000502:physiology*; D016667:Bacterial Capsules / Q000502:physiology*; D001426:Bacterial Proteins / Q000502:physiology; D006863:Hydrogen-Ion Concentration; D008055:Lipids / Q000502:physiology; D011135:Polysaccharides, Bacterial / Q000502:physiology*; D013696:Temperature
D001426:Bacterial Proteins; D008055:Lipids; D011135:Polysaccharides, Bacterial