Characterization of exopolymers of aquatic bacteria by pyrolysis-mass spectrometry.
Exopolymers from a diverse collection of marine and freshwater bacteria were characterized by pyrolysis-mass spectrometry (Py-MS).  Py-MS provides spectra of pyrolysis fragments that are characteristic of the original material.  Analysis of the spectra by multivariate statistical techniques (principal component and canonical variate analysis) separated these exopolymers into distinct groups.  Py-MS clearly distinguished characteristic fragments, which may be derived from components responsible for functional differences between polymers.  The importance of these distinctions and the relevance of pyrolysis information to exopolysaccharide function in aquatic bacteria is discussed.
Ford
T
T
Laboratory of Microbial Ecology, Division of Applied Sciences, Harvard University, Cambridge, Massachusetts 02138.
Sacco
E
E
Black
J
J
Kelley
T
T
Goodacre
R
R
Berkeley
R C
RC
Mitchell
R
R
D002241:Carbohydrates / Q000032:analysis; D006088:Gram-Negative Aerobic Bacteria / Q000737:chemistry* / Q000302:isolation & purification; D013058:Mass Spectrometry; D011135:Polysaccharides, Bacterial / Q000032:analysis / Q000737:chemistry*; D011506:Proteins / Q000032:analysis; D013824:Thermus / Q000737:chemistry* / Q000302:isolation & purification; D014871:Water Microbiology
D002241:Carbohydrates; D011135:Polysaccharides, Bacterial; D011506:Proteins