The Rhesus monkey as a model for testing the immunological effects of space flight.
The Rhesus monkey has been proposed as a model for the effects of space flight on immunity.  In order to determine the feasibility of the use of the Rhesus monkey as a model, we studied the use of Rhesus monkey cells for immunological procedures that have been shown to be affected by space flight in both rodents and humans.  We have shown that both lymph node cells and peripheral blood leukocytes can be stained with monoclonal antibodies to detect the following surface markers:  CD4, CD-8, Ia and surface immunoglobulin.  Also, the level of Ia antigen expression was increased by treatment of the cells with human interferon-gamma.  In addition, cells were induced to produce interferons and interleukins.  Isolated neutrophils also demonstrated increased oxidative burst.  These data indicate that the Rhesus monkey will be a useful model for space flight studies of immunity.
Sonnenfeld
G
G
Department of Microbiology and Immunology, University of Louisville, KY 40292.
Schaffar
L
L
Schmitt
D A
DA
Peres
C
C
Miller
E S
ES
D000818:Animals; D000917:Antibody Formation / Q000502:physiology; D000954:Antigens, Surface; D015704:CD4 Antigens / Q000032:analysis / Q000276:immunology / Q000378:metabolism; D016827:CD8 Antigens / Q000032:analysis / Q000276:immunology / Q000378:metabolism; D003429:Cross Reactions; D000949:Histocompatibility Antigens Class II / Q000032:analysis / Q000276:immunology / Q000378:metabolism; D007111:Immunity, Cellular / Q000502:physiology*; D007371:Interferon-gamma / Q000494:pharmacology; D007962:Leukocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D008253:Macaca mulatta / Q000276:immunology / Q000502:physiology*; D008297:Male; D011947:Receptors, Antigen, B-Cell / Q000032:analysis / Q000276:immunology / Q000378:metabolism; D012107:Research Design; D013026:Space Flight; D014893:Weightlessness
D000954:Antigens, Surface; D015704:CD4 Antigens; D016827:CD8 Antigens; D000949:Histocompatibility Antigens Class II; D011947:Receptors, Antigen, B-Cell; D007371:Interferon-gamma