title: Identification and expression of a new sulfhydryl oxidase SOx-3 during the cell cycle and the estrus cycle in uterine cells.
abstract: Using differential hybridization of a guinea pig endometrial cell cDNA library, a potentially negatively estrogen-regulated gene, SOX-3, was isolated. According to the nucleotide and protein sequence similarities, SOx-3 belonged to the FAD-linked sulfhydryl oxidase family containing the egg white sulfhydryl oxidase, the rat seminal vesicle sulfhydryl oxidase-2 SOx-2, the quiescence-inducible protein hQ6. The SOX-3 transcript in the guinea pig as well as 5 different mRNAs in human tissues appeared differentially expressed in the tissues studied. In secondary endometrial cell culture, the SOX-3 mRNA level increased during a serum depletion-induced quiescence, decreased when cells enter the G1 phase after serum stimulation, and was restored during the S and G2/M phases. Thus, SOX-3 could be implicated in the negative cell cycle control. The SOx-3 protein appeared to be specific of epithelial cells in the uterus. Its expression level varied during the estrus cycle in the guinea pig, suggesting a regulation by steroid hormones.
authors.affiliation: Institut d'Etude et de Transfert de Gènes, Bâtiment INSERM, 25030 Besançon cedex, France.
authors.forename: J F
authors.initials: JF
authors.lastname: Musard
authors.forename: M
authors.initials: M
authors.lastname: Sallot
authors.forename: P
authors.initials: P
authors.lastname: Dulieu
authors.forename: A
authors.initials: A
authors.lastname: Fraîchard
authors.forename: C
authors.initials: C
authors.lastname: Ordener
authors.forename: J P
authors.initials: JP
authors.lastname: Remy-Martin
authors.forename: M
authors.initials: M
authors.lastname: Jouvenot
authors.forename: P
authors.initials: P
authors.lastname: Adami
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002453:Cell Cycle / Q000502:physiology; D018076:DNA, Complementary / Q000032:analysis; D004268:DNA-Binding Proteins / Q000737:chemistry; D004717:Endometrium / Q000378:metabolism; D004967:Estrogens / Q000494:pharmacology; D004971:Estrus / Q000378:metabolism*; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D015723:Gene Library; D006168:Guinea Pigs; D024002:HMGB Proteins; D006801:Humans; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000737:chemistry; D009928:Organ Specificity; D010088:Oxidoreductases / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification*; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D055748:SOXB1 Transcription Factors; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors; D014599:Uterus / Q000166:cytology / Q000201:enzymology*; D050862:Oxidoreductases Acting on Sulfur Group Donors
chemical_list: D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D004967:Estrogens; D024002:HMGB Proteins; D009687:Nuclear Proteins; D010088:Oxidoreductases; D012333:RNA, Messenger; D055748:SOXB1 Transcription Factors; D014157:Transcription Factors; D050862:Oxidoreductases Acting on Sulfur Group Donors; C527782:SOX2 protein, human; C527784:Sox2 protein, rat