title: Matrix metalloproteinase activities of turkey (Meleagris gallopavo) bile.
abstract: The bile from turkey (Meleagris gallopavo) gall bladders was found to contain substantial matrix metalloproteinase (MMP) activities using gelatin, collagen, and casein substrate zymography, [3H]labeled collagen degradation assays, and gelatin-agarose affinity purification. Five major bands corresponding to approximate M(w) of 64, 60, 46, 40 and 36 kDa showed gelatinolytic activities. On incubation with p-aminophenylmercuric acetate or thimerosal, the densities of both the 64- and 46-kDa bands decreased with increasing intensities of the 60- and 40-kDa bands. Both the 64- and 60-kDa bands showed collagenolytic activities whereas the caseinolytic activities appeared as diffuse bands corresponding to M(w) of approximately 60, 40 and 36 kDa. Using [3H]collagen as substrate, the bile enzymes showed both a time and concentration-dependent degradation, which could be inhibited by the MMP inhibitors such as EDTA, phenanthroline, and N-[(2R)-2-(hydroxyamido carbonylmethyl)-4-methylpentanonyl]-L-tryptophan methylamide, but not by serine and cysteine protease inhibitors like trans-epoxysuccinyl-L-leucylamido-(4-guanidino)butane, phenylmethylsulfonyl fluoride or leupeptin. Both 60- and the 40-kDa gelatinolytic bands showed affinity adsorption to a gelatin-agarose matrix. The physiological roles of bile MMPs are not clear, but their involvement in the digestive functions of birds are likely.
authors.affiliation: USDA, ARS, PPPSRU, Poultry Science Center, University of Arkansas, Fayetteville, AR 72701, USA. nrath@uark.edu
authors.forename: N C
authors.initials: NC
authors.lastname: Rath
authors.forename: W E
authors.initials: WE
authors.lastname: Huff
authors.forename: G R
authors.initials: GR
authors.lastname: Huff
authors.forename: J M
authors.initials: JM
authors.lastname: Balog
authors.forename: H
authors.initials: H
authors.lastname: Xie
mesh_terms: D000818:Animals; D001646:Bile / Q000201:enzymology*; D015153:Blotting, Western; D002645:Chickens; D002846:Chromatography, Affinity; D003094:Collagen / Q000378:metabolism*; D017364:Collagenases / Q000032:analysis / Q000276:immunology; D005780:Gelatin / Q000737:chemistry; D053509:Matrix Metalloproteinase 13; D020782:Matrix Metalloproteinases / Q000378:metabolism*; D012685:Sepharose / Q000737:chemistry; D014422:Turkeys
chemical_list: D005780:Gelatin; D003094:Collagen; D012685:Sepharose; D017364:Collagenases; D053509:Matrix Metalloproteinase 13; D020782:Matrix Metalloproteinases