title: Cyclohexene nucleic acids (CeNA) form stable duplexes with RNA and induce RNase H activity.
abstract: Cyclohexene nucleic acids (CeNA) were synthesized using classical phosporamidite chemistry. Incorporation of a cyclohexene nucleo-side in a DNA chain leads to an increase in stability of the DNA/RNA duplex. CeNA is stable against degradation in serum. A CeNA/RNA hybrid is able to activate E. Coli RNase H. resulting in cleavage of the RNA strand.
authors.affiliation: Laboratory for Medicinal Chemistry, Rega Institute, Katholieke Universiteit Leuven, Minderbroedersstraat 10, B-3000 Leuven, Belgium.
authors.forename: J
authors.initials: J
authors.lastname: Wang
authors.forename: B
authors.initials: B
authors.lastname: Verbeure
authors.forename: I
authors.initials: I
authors.lastname: Luyten
authors.forename: M
authors.initials: M
authors.lastname: Froeyen
authors.forename: C
authors.initials: C
authors.lastname: Hendrix
authors.forename: H
authors.initials: H
authors.lastname: Rosemeyer
authors.forename: F
authors.initials: F
authors.lastname: Seela
authors.forename: A
authors.initials: A
authors.lastname: Van Aerschot
authors.forename: P
authors.initials: P
authors.lastname: Herdewijn
mesh_terms: D002942:Circular Dichroism; D003510:Cyclohexanes / Q000737:chemistry* / Q000378:metabolism; D053138:Cyclohexenes; D004247:DNA / Q000737:chemistry; D004789:Enzyme Activation; D004926:Escherichia coli / Q000201:enzymology; D009696:Nucleic Acids / Q000737:chemistry* / Q000378:metabolism; D012313:RNA / Q000737:chemistry* / Q000378:metabolism; D016914:Ribonuclease H / Q000378:metabolism*; D013402:Sugar Alcohols / Q000737:chemistry
chemical_list: D003510:Cyclohexanes; D053138:Cyclohexenes; D009696:Nucleic Acids; D013402:Sugar Alcohols; C052568:cyclohexene; D012313:RNA; D004247:DNA; D016914:Ribonuclease H