[Expression of hepatitis A virus recombinant proteins in Escherichia coli].
On the basis of coding regions on the fragments of genes P1 and P2 of hepatitis A virus (HAV) recombinant proteins of this virus have been synthesized in the prokaryotic expressing system of E. coli, isolated and studied with the use of sera obtained from hepatitis A patients. The capacity of HAV recombinant proteins for binding with the sera of patients with hepatitis A in the acute stage has been shown with the use of immunoblotting and the indirect solid-phase enzyme immunoassay. The results obtained in this investigation are discussed in the light of the possible use of recombinant proteins for the detection of HAV markers.
Ternovoĭ
V A
VA
Institute of Molecular Biology-State Research Center Vector, Koltsovo, Novosibirsk Region, Russia.
Bondarenko
T Iu
TIu
Bondarchuk
V B
VB
Maĭdaniuk
A G
AG
Netesov
S V
SV
D001798:Blood Proteins / Q000378:metabolism; D015153:Blotting, Western; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D016679:Genome, Viral; D006506:Hepatitis A / Q000097:blood; D006507:Hepatovirus / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007124:Immunoenzyme Techniques; D020815:Polyproteins / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
D001798:Blood Proteins; D020815:Polyproteins; D011994:Recombinant Proteins; D014764:Viral Proteins