title: Development of autonomous control in a closed microbial bioreactor.
abstract: Space-based life support systems which include ecological components will rely on sophisticated hardware and software to monitor and control key system parameters. Autonomous closed artificial ecosystems are useful for research in numerous fields. We are developing a bioreactor designed to study both microbe-environment interactions and autonomous control systems. Currently we are investigating N-cycling and N-mass balance in closed microbial systems. The design features of the system involve real-time monitoring of physical parameters (e.g. temperature, light), growth solution composition (e.g. pH, NOx, CO2), cell density and the status of important hardware components. Control of key system parameters is achieved by incorporation of artificial intelligence software tools that permit autonomous decision-making by the instrument. These developments provide a valuable research tool for terrestrial microbial ecology, as well as a testbed for implementation of artificial intelligence concepts. Autonomous instrumentation will be necessary for robust operation of space-based life support systems, and for use on robotic spacecraft. Sample data acquired from the system, important features of software components, and potential applications for terrestrial and space research will be presented.
authors.affiliation: SETI Institute, Moffett Field, CA 94035-1000, USA.
authors.forename: D T
authors.initials: DT
authors.lastname: Smernoff
authors.forename: R L
authors.initials: RL
authors.lastname: Mancinelli
mesh_terms: D001185:Artificial Intelligence; D019149:Bioreactors; D015169:Colony Count, Microbial; D003201:Computers; D018504:Ecological Systems, Closed; D004783:Environmental Microbiology; D004867:Equipment Design; D008021:Life Support Systems / Q000295:instrumentation*; D009391:Nephelometry and Turbidimetry; D009584:Nitrogen / Q000737:chemistry / Q000378:metabolism; D011551:Pseudomonas fluorescens / Q000254:growth & development / Q000378:metabolism; D012371:Robotics; D012984:Software; D018511:Systems Integration; D013696:Temperature
chemical_list: D009584:Nitrogen