Derivatization and template-guided ligation of oligodeoxyribonucleotides using cyanogen bromide and N-substituted morpholines.
Cyanogen bromide has been found to induce the template-guided condensation of oligonucleotides only in the presence of N-substituted morpholines. Based on 31P, 1H and 13C NMR spectroscopy data, the mechanism of the phosphomonoester group activation by cyanogen bromide in N-substituted morpholine buffers is suggested. It has also been shown that BrCN can be used for the synthesis of oligonucleotide derivatives in aqueous solution.
Shabarova
Z A
ZA
Chemistry Department, Lomonosov Moscow State University, Russia.
Fedorova
O A
OA
Dolinnaya
N G
NG
Gottikh
M B
MB
D002021:Buffers; D003488:Cyanogen Bromide / Q000737:chemistry*; D004247:DNA / Q000737:chemistry; D019143:Evolution, Molecular; D009025:Morpholines / Q000737:chemistry*; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D009841:Oligonucleotides / Q000138:chemical synthesis*; D013698:Templates, Genetic
D002021:Buffers; D009025:Morpholines; D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; D004247:DNA; D003488:Cyanogen Bromide