title: [Behavioral and electrophysiological analysis of the choline-positive effect of nootropic dipeptide acylproline (GVS-111)].
abstract: The behavioral experiments using a passive avoidance learning model showed that the new cognition-enhancing acyl-prolyn containing dipeptide GVS-111 promotes recovery of the test performance in animals with a long-term memory deficit caused by the M-cholinolytic scopolamine (1 mg/kg/day scopolamine for 20 days, followed by 0.5 mg/kg/day GVS-111 for 10 days). At the same time, GVS-111 increased the duration of tremor induced by the M-cholinomimetic arecoline. The results of electrophysicological experiments showed that GVS-111 in a concentration range from 10(-11) to 10(-9) M increased amplitude of the neural response to acetylcholine (Ach) microappications in 75% of the isolated neurons of Helix Pomatum and produced a predominantly facilitating effect upon the endoneuronal pacemaker activity. The effect of GVS-111 upon the Ach response in a part of neurons was attenuated or even blocked by scopolamine, and in the other neurons--by the N-cholinolytic d-tubocurarine. This fact indicates that both muscarinic and nicotinic receptors are involved in the mechanism of the cholino-sensitizing action of GVS-111 upon the neuronal activity.
authors.affiliation: Laboratory of Psychopharmacology, Institute of Pharmacology, Russian Academy of Medical Sciences, Baltiiskaya ul. 8, Moscow, 125315 Russia.
authors.forename: R U
authors.initials: RU
authors.lastname: Ostrovskaia
authors.forename: T Kh
authors.initials: TKh
authors.lastname: Mirzoev
authors.forename: F A
authors.initials: FA
authors.lastname: Firova
authors.forename: S S
authors.initials: SS
authors.lastname: Trofimof
authors.forename: T A
authors.initials: TA
authors.lastname: Gudasheva
authors.forename: T N
authors.initials: TN
authors.lastname: Grechenko
authors.forename: E F
authors.initials: EF
authors.lastname: Gutyrchik
authors.forename: E B
authors.initials: EB
authors.lastname: Barkova
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D001362:Avoidance Learning / Q000187:drug effects*; D004151:Dipeptides / Q000494:pharmacology*; D004594:Electrophysiology; D017952:Ganglia, Invertebrate / Q000166:cytology; D006372:Helix, Snails; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D018727:Muscarinic Antagonists / Q000494:pharmacology; D009474:Neurons / Q000502:physiology; D018697:Nootropic Agents / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012601:Scopolamine / Q000494:pharmacology
chemical_list: D004151:Dipeptides; D018727:Muscarinic Antagonists; D018697:Nootropic Agents; C103871:ethyl phenylacetyl-Pro-Gly; D012601:Scopolamine; D000109:Acetylcholine