[The comparison of genetic divergences and its relationships to heterosis revealed by SSR and RFLP markers in rice (Oryza sativa L.)].
Genetic divergences of 57 entries from 6 ecotypes of rice were surveyed by PCR analysis with 48 pairs of SSR and by Southern analysis by 50 RFLP probes. It was indicated that SSR markers detected polymorphism efficiently in comparison to RFLP markers, considering the allele numbers per locus and average gene diversity. Among all of 1,596 pairs, 1,452 pairs of genetic distances based on SSR data (GDSSR) were higher than the genetic distance based on RFLP data (GDRFLP) by average of 0.2492. The average GDSSR of entries within indica subspecies was 0.5483, but GDRFLP was only 0.2776. And the GDSSR and GDRFLP of entries with japonica subspecies were 0.577, 0.2405 respectively. The average GDSSR between varieties of interspecies was 0.8074, and the average GDRFLP was 0.7310. It is suggested that the GDSSR between varieties of introspecies or interspecies are higher than that of GDRFLP. The analysis of correlation between GDSSR and GDRFLP of any entry with other 56 entries indicated that the correlation coefficient for typical Indica or Japonica varieties were higher, but lower for Indica-like or Japonica-like materials. It was also found that the relationships between genetic distance based on these two kinds of markers and the yield of hybrid performance were not significant, but most of the correlation coefficient between heterosis and GDRFLP or GDSSR arrived at highly significant level.
Zhu
Z F
ZF
Department of Plant Genetics and Breeding, China Agricultural University, Beijing 100094, China.
Sun
C Q
CQ
Jiang
T B
TB
Fu
Q
Q
Wang
X K
XK
D014644:Genetic Variation; D006823:Hybrid Vigor; D018598:Minisatellite Repeats; D012275:Oryza / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length