Widespread distribution of adenovirus-transduced monkey amniotic epithelial cells after local intracerebral injection: implication for cell-mediated therapy for lysosome storage disorders.
Cell-mediated therapy for mucopolysaccharidosis type VII (MPSVII) was studied using monkey amniotic epithelial cells (mAEC). The cells were transduced with a recombinant adenovirus expressing human beta-glucuronidase (GUSB), and cells overexpressing GUSB were generated. The cells expressed 2000-fold higher activities than the endogenous GUSB activities of nontransduced mAEC, demonstrating that mAEC were successfully transduced with adenoviral vectors. These cells also secreted high levels of GUSB. To clarify the cross-correction of GUSB secreted from mAEC, the conditioned medium containing high levels of GUSB was added into the medium for culturing human or murine fibroblasts established from an MPSVII patient or a mouse model of the disease. Dramatic increases in GUSB activities were observed in both fibroblasts. We then transplanted the cells transduced with an adenovirus expressing LacZ into the caudate-putamen of monkey brain. Survival and distribution of the transplanted cells 1 month after the treatment were evaluated. Histochemical analysis showed that LacZ-positive cells were widely distributed in the brain, suggesting that the transplanted cells had migrated and were distributed even at regions far from the implantation site. These findings suggest that local intracerebral engraftment of genetically engineered amniotic epithelial cells is favorable for the treatment of lysosome storage disorders, whose pathological abnormalities are not restricted to specific regions of the brain.
Kosuga
M
M
Department of Genetics, National Children's Medical Research Center, Tokyo, Japan.
Takahashi
S
S
Tanabe
A
A
Fujino
M
M
Li
X K
XK
Suzuki
S
S
Yamada
M
M
Kakishita
K
K
Ono
F
F
Sakuragawa
N
N
Okuyama
T
T
D000256:Adenoviridae / Q000235:genetics* / Q000378:metabolism; D000650:Amnion / Q000166:cytology*; D000818:Animals; D001921:Brain / Q000166:cytology* / Q000378:metabolism; D017690:Cell Transplantation; D002478:Cells, Cultured; D002552:Cerebral Ventricles / Q000166:cytology; D017077:Culture Media, Conditioned; D004847:Epithelial Cells / Q000502:physiology* / Q000637:transplantation* / Q000821:virology; D005347:Fibroblasts / Q000502:physiology; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D005966:Glucuronidase / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007763:Lac Operon / Q000235:genetics; D008252:Macaca fascicularis; D051379:Mice; D016538:Mucopolysaccharidosis VII / Q000628:therapy; D017527:Receptor, IGF Type 2 / Q000378:metabolism; D014161:Transduction, Genetic; D014184:Transplantation, Homologous
D017077:Culture Media, Conditioned; D017527:Receptor, IGF Type 2; D005966:Glucuronidase