Alu-mediated inactivation of the human CMP- N-acetylneuraminic acid hydroxylase gene.
Inactivation of the CMP-N-acetylneuraminic acid hydroxylase gene has provided an example of human-specific genomic mutation that results in a widespread biochemical difference between human and nonhuman primates. We have found that, although a region containing a 92-bp exon and an AluSq element in the hydroxylase gene is intact in all nonhuman primates examined, the same region in the human genome is replaced by an AluY element that was disseminated at least one million years ago. We propose a mechanistic model for this Alu-mediated replacement event, which deleted the 92-bp exon and thus inactivated the human hydroxylase gene. It is suggested that Alu elements have played potentially important roles in genotypic and phenotypic evolution in the hominid lineage.
Hayakawa
T
T
Department of Biosystems Science, Graduate University for Advanced Studies (Sokendai), Hayama, Kanagawa 240-0193, Japan.
Satta
Y
Y
Gagneux
P
P
Varki
A
A
Takahata
N
N
D020087:Alu Elements / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D019143:Evolution, Molecular; D005091:Exons; D006071:Gorilla gorilla / Q000235:genetics; D006801:Humans; D006922:Hylobates / Q000235:genetics; D008253:Macaca mulatta; D006899:Mixed Function Oxygenases / Q000235:genetics*; D008969:Molecular Sequence Data; D002679:Pan troglodytes / Q000235:genetics; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011148:Pongo pygmaeus / Q000235:genetics; D011323:Primates / Q000235:genetics; D017384:Sequence Deletion
D006899:Mixed Function Oxygenases; C074383:CMPacetylneuraminate monooxygenase