Effect of modulator sorption on gradient shape in ion-exchange chromatography.
Mobile phase additives, or modulators, are used in gradient elution chromatography to facilitate separation and reduce separation time. The modulators are usually assumed to be linearly adsorbed or unadsorbed. Here, the consequences of nonlinear modulator adsorption are examined for ion-exchange gradient elution through a series of simulations. Even when the buffer salt is identical to the modulator salt, gradient deformation is observed; the extent of deformation increases as the volume of the feed is increased. When the modulator salt is different from the buffer salt, unusual effects are observed, and the chromatograms are quite different from those predicted by classical gradient elution theory. In particular, local increases in the buffer concentration are found between feed bands, and serve to improve the separation. These effects become more pronounced as the feed volume increases, and could therefore prove valuable in preparative applications.
Velayudhan
A
A
Department of Bioresource Engineering, Oregon State University, Corvallis 97331-3906, USA.
Ladisch
M R
MR
D000327:Adsorption; D002021:Buffers; D002411:Cation Exchange Resins / Q000737:chemistry; D002852:Chromatography, Ion Exchange; D002919:Chymotrypsinogen / Q000737:chemistry; D003574:Cytochrome c Group / Q000737:chemistry; D008956:Models, Chemical; D009113:Muramidase / Q000737:chemistry; D010710:Phosphates / Q000737:chemistry*; D012492:Salts / Q000737:chemistry*
D002021:Buffers; D002411:Cation Exchange Resins; D003574:Cytochrome c Group; D010710:Phosphates; D012492:Salts; D002919:Chymotrypsinogen; D009113:Muramidase; C018279:sodium phosphate