A new continuous cell line from the mosquito Psorophora confinnis (Diptera: Culicidae) and its susceptibility to infections with some arboviruses.
A new cell line, PC-0199-BR, was established from embryonated eggs of the mosquito Psorophora confinnis. To date (September 2000) it has had 62 continuous passages. This is the first report of a cell line of mosquitoes belonging to the genus Psorophora. Cell growth initially was achieved in the MM/VP12 medium, supplemented with 20% fetal bovine serum; however, the subcultures were later adapted to Grace's medium with 10% fetal bovine serum. Cell morphology in the primary cultures was heterogeneous; but later in the established cell line, the predominant cell type was epithelioid. Cultured cells were predominantly diploid (2n=6); however, chromosome abnormalities were observed in a small proportion of the cells in later passages. C and G band patterns were also determined in the karyotype. The cell line isozyme profiles coincided with pupae and adult samples of the species taken from the same colony. A preliminary arbovirus susceptibility study for the cell line was undertaken. No evidence was observed of contamination of the cell line with bacteria, fungi or mycoplasma.
Bello
F J
FJ
Departamento de Biología y Química, Universidad De La Salle, Bogotá, D.C., Colombia. fbellog@atenea.lasalle.edu.co
Rodríguez
J A
JA
Escovar
J
J
Olano
V A
VA
Morales
A
A
González
M
M
Rey
G
G
D000818:Animals; D001103:Arboviruses; D002460:Cell Line / Q000737:chemistry / Q000166:cytology / Q000821:virology; D009033:Culicidae / Q000235:genetics* / Q000821:virology; D013997:Time Factors