title: Delayed fertilization of anuran amphibian (Xenopus) eggs leads to reduced numbers of primordial germ cells.
abstract: Several media were tested for the extent to which they promoted high fertilization efficiencies in ovulated, stripped Xenopus eggs.  One medium was selected for maintaining eggs in a 'delayed fertilization' (DelF) condition.  DelF eggs displayed several unusual characteristics, including shift of the center of gravity, prominent sperm entrance site, and occasional polyspermy.  The frequency of normal pattern formation varied according to the length of time eggs were maintained in the DelF condition.  Various developmental abnormalities were observed during gastrulation, neurulation, and organogenesis.  Most abnormalities appeared, however, to be related to morphogenesis of the endoderm.  Primordial germ cell (PGC) development was examined in DelF eggs which displayed normal external morphological features at the swimming tadpole stage.  PGC counts were usually normal in short-duration (eg, 5 hr) DelF eggs, but frequently substantially reduced or completely diminished in longer-duration (eg, 25h) tadpoles.  Six spawnings were compared and shown to exhibit considerable variability in fertility, morphogenesis, and PGC development.  Yolk platelet shifts and developmental parameters were examined in two additional spawnings.  The subcortical cytoplasm in which the germ plasm is normally localized appeared to be disrupted in longer duration DelF eggs.  That observation may account for low PGC counts in DelF tadpoles.
authors.affiliation: Department of Biology and Anatomy Section, Indiana University, Bloomington, USA.
authors.forename: M
authors.initials: M
authors.lastname: Wakahara
authors.forename: A W
authors.initials: AW
authors.lastname: Neff
authors.forename: G M
authors.initials: GM
authors.lastname: Malacinski
mesh_terms: D000818:Animals; D003470:Culture Media; D004625:Embryo, Nonmammalian; D005260:Female; D005306:Fertilization / Q000502:physiology*; D005854:Germ Cells / Q000254:growth & development*; D007814:Larva / Q000254:growth & development; D008297:Male; D009865:Oocytes / Q000166:cytology*; D010063:Ovum / Q000254:growth & development; D014981:Xenopus / Q000033:anatomy & histology / Q000196:embryology*
chemical_list: D003470:Culture Media