Chronic inhibition of NO synthesis per se promotes structural intimal remodeling of the rat aorta.
OBJECTIVE
We characterized, using histomorphometry and transmission and scanning electron microscopy, the intimal remodeling of the thoracic aorta of normocholesterolemic young rats chronically-treated with N(omega)-nitro-L-arginine methylester (L-NAME) and examined the question whether these changes were caused by the lack of NO per se or by the hypertension which L-NAME administration induces.


METHODS
Male Wistar rats were divided randomly into three sets: control group, standard diet/L-NAME-treated group, and standard diet/L-NAME + captopril-treated group.


RESULTS
The treatment of rats with L-NAME for 4 weeks resulted in increased blood pressure (by 32% at the end of the treatment) as compared with the control value and intimal remodeling comprising a continuous layer of enlarged endothelial cells with irregular nuclear and cytoplasmic contours, lying over a thickened layer of fibrocollagenous support tissue focally expanded with lymphomononuclear cells and mainly diffuse foci of smooth muscle cells. In addition, the NO synthase inhibition caused a marked thickened tunica intima (150% thicker than the control value) and a significantly augmented intima : media ratio (126% higher than the control value). On the other hand, captopril prevented hypertension in rats simultaneously treated with L-NAME as compared with controls, and induced intimal remodeling comprising the same qualitative changes as those observed in L-NAME-treated rats. The tunica intima of l-NAME + captopril-treated rats was moderately thickened (60% increase in comparison with that of controls and 65% thinner as compared with L-NAME-treated rats). In the same way, the mean intima : media ratio of rats concomitantly treated with L-NAME and captopril was moderately increased (45% more) as compared with controls and significantly lower in comparison with rats administered L-NAME alone (36% less).


CONCLUSIONS
Chronic inhibition of NO synthesis per se promotes structural intimal remodeling of the rat aorta, which is potentiated by L-NAME-induced hypertension. Most important, the present findings favor the idea that blockade of NO synthesis by causing intimal remodeling might be a primary cause, as individual biologic phenomenon, in the development of an atherosclerotic plaque.
Rossi
M A
MA
Department of Pathology, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil. marossi@fmrp.usp.br
Colombini-Netto
M
M
D000818:Animals; D000959:Antihypertensive Agents / Q000494:pharmacology; D001013:Aorta, Thoracic / Q000187:drug effects / Q000473:pathology / Q000503:physiopathology*; D002216:Captopril / Q000494:pharmacology; D004357:Drug Synergism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006973:Hypertension / Q000139:chemically induced / Q000503:physiopathology / Q000517:prevention & control; D008297:Male; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009569:Nitric Oxide / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D017539:Tunica Intima / Q000187:drug effects / Q000473:pathology / Q000503:physiopathology*
D000959:Antihypertensive Agents; D004791:Enzyme Inhibitors; D009569:Nitric Oxide; D002216:Captopril; D019331:NG-Nitroarginine Methyl Ester