title: Cerebral arteriolar structure and function in pinealectomized rats.
abstract: We examined cerebral arteriolar structure and autoregulation of cerebral blood flow (CBF) in control (n = 8), sham-operated (n = 8), pinealectomized (n = 10), and pinealectomized plus melatonin-treated (0.51 +/- 0.01 mg x kg(-1) x day(-1) in drinking water, n = 9) young Wistar rats. The lower limit of CBF autoregulation (LLCBF) was determined by measurement of CBF (in arbitrary units, laser Doppler) during stepwise hypotensive hemorrhage; the arteriolar internal diameter (ID; in microm, cranial window) was also measured. Measurements of ID were repeated during a second stepwise hypotension after smooth muscle cell deactivation (67 mmol/l EDTA). The cross-sectional area (CSA) was measured by histometry. CSA and EDTA-induced vasodilatation decreased after pinealectomy (517 +/- 21 vs. 819 +/- 40 microm(2) in sham and 829 +/- 55 microm(2) in control, P < 0.05, and 81 +/- 4 vs. 102 +/- 5 microm in sham and 104 +/- 4 microm in control, P < 0.05, respectively) and were restored by melatonin (924 +/- 39 microm(2) and 102 +/- 5 microm, respectively). These results suggest that melatonin deprival makes the arteriolar wall thinner and stiffer. However, these changes had little effect on LLCBF. In conclusion, pinealectomy of young rats induces atrophy and decreases distensibility of the cerebral arteriolar wall; these effects are prevented by melatonin. They do not modify LLCBF.
authors.affiliation: Cardiovascular Research Group, Faculté de Pharmacie de l'Université Henri Poincaré-Nancy I, 54000 Nancy, France.
authors.forename: O
authors.initials: O
authors.lastname: Régrigny
authors.forename: F
authors.initials: F
authors.lastname: Dupuis
authors.forename: J
authors.initials: J
authors.lastname: Atkinson
authors.forename: P
authors.initials: P
authors.lastname: Limiñana
authors.forename: E
authors.initials: E
authors.lastname: Scalbert
authors.forename: P
authors.initials: P
authors.lastname: Delagrange
authors.forename: J M
authors.initials: JM
authors.lastname: Chillon
mesh_terms: D000818:Animals; D001160:Arterioles / Q000033:anatomy & histology / Q000502:physiology / Q000503:physiopathology; D002560:Cerebrovascular Circulation / Q000502:physiology*; D006706:Homeostasis / Q000502:physiology; D007022:Hypotension / Q000503:physiopathology; D008297:Male; D008550:Melatonin / Q000502:physiology; D010870:Pineal Gland / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D012016:Reference Values; D014664:Vasodilation
chemical_list: D008550:Melatonin