title: Effect of acetophenone derived Mannich bases on cellular glutathione level in Jurkat cells. A possible mechanism of action.
abstract: The effect of the acetophenone derived mono Mannich bases 1-3 and bis Mannich base 7 (bis derivative of compound 3) on cellular glutathione level was investigated in Jurkat cells. The cells were exposed to the compounds in phosphate buffered saline for 1 h in 37 degrees C with gentle shaking and then glutathione level was measured. Especially, mono Mannich base 3 and its bis derivative 7 decreased total glutathione level in a dose-dependent manner. The results provide further support for the thiol alkylation mechanism explaining the cytotoxic activity of Mannich bases.
authors.affiliation: Department of Physiology, University of Kuopio, Kuopio, Finland.
authors.forename: M
authors.initials: M
authors.lastname: Gul
authors.forename: H I
authors.initials: HI
authors.lastname: Gul
authors.forename: J
authors.initials: J
authors.lastname: Vepsalainen
authors.forename: E
authors.initials: E
authors.lastname: Erciyas
authors.forename: O
authors.initials: O
authors.lastname: Hanninen
mesh_terms: D000098:Acetophenones / Q000737:chemistry*; D000478:Alkylation; D005978:Glutathione / Q000378:metabolism*; D006801:Humans; D019169:Jurkat Cells; D008352:Mannich Bases / Q000737:chemistry*
chemical_list: D000098:Acetophenones; D008352:Mannich Bases; D005978:Glutathione