title: Direct electrochemical determination of pyruvate in human sweat by capillary zone electrophoresis.
abstract: Capillary zone electrophoresis was employed for the determination of pyruvate in human sweat using electrochemical detection with a carbon fiber microdisk bundle electrode at a constant potential of 1.60 V vs. saturated calomel electrode. The optimum separation conditions are 3.6 x 10(-3) mol/L Na2HPO4-1.4 x 10(-3) mol/L NaH2PO4 (pH 7.2) for the buffer solution, and 18 kV for the separation voltage. The limits of detection of pyruvate are 8.0 x 10(-6) mol/L or 24 fmol (S/N = 3) for the injection voltage of 6 kV and the injection time of 10 s. The relative standard deviation is 2.0% for the migration time and 5.7% for the electrophoretic peak current. The method was applied to determining pyruvate in human sweat.
authors.affiliation: Laboratory of Analytical Science, School of Chemistry, Shandong University, Jinan, PR China.
authors.forename: M
authors.initials: M
authors.lastname: Jin
authors.forename: Q
authors.initials: Q
authors.lastname: Dong
authors.forename: R
authors.initials: R
authors.lastname: Dong
authors.forename: W
authors.initials: W
authors.lastname: Jin
mesh_terms: D004563:Electrochemistry; D019075:Electrophoresis, Capillary / Q000379:methods* / Q000592:standards; D006801:Humans; D016014:Linear Models; D019289:Pyruvic Acid / Q000032:analysis*; D015203:Reproducibility of Results; D013542:Sweat / Q000737:chemistry*
chemical_list: D019289:Pyruvic Acid