title: Cytoskeleton-amyloplast interactions in sweet clover.
abstract: The distribution of organelles within columella cells of sweet clover was examined by transmission electron microscopy following growth under static or clinorotating conditions.  A developmentally conditioned polarity was observed, with a proximal location of the nucleus and a distal accumulation of the endoplasmic reticulum.  This polarity was insensitive to clinorotation.  In contrast, clinorotation altered the location of amyloplasts.  Application of cytoskeletal poisons (colchicine, cytochalasin D, taxol, and phalloidin), especially during clinorotation, had interesting effects on the maintenance of columella cell polarity, with a profound effect on the extent, location, and structure of the endoplasmic reticulum.  The site of cytoskeletal interactions with sedimenting amyloplasts is thought to be the amyloplast envelope.  An envelope fraction, having over 17 polypeptides, was isolated using immobilized antibody technology, and will provide a means of assessing the role of specific peptides in cytoskeleton/amyloplast interactions.
authors.affiliation: Division of Biology, Kansas State University, Manhattan 66506-4901, USA.
authors.forename: J A
authors.initials: JA
authors.lastname: Guikema
authors.forename: E
authors.initials: E
authors.lastname: Hilaire
authors.forename: W R
authors.initials: WR
authors.lastname: Odom
mesh_terms: D016764:Cell Polarity / Q000187:drug effects / Q000502:physiology*; D003078:Colchicine / Q000494:pharmacology; D015638:Cytochalasin D / Q000494:pharmacology; D003599:Cytoskeleton / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D004721:Endoplasmic Reticulum / Q000737:chemistry / Q000648:ultrastructure; D006112:Gravitation; D041743:Herb-Drug Interactions; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D015388:Organelles / Q000378:metabolism / Q000502:physiology / Q000648:ultrastructure*; D017239:Paclitaxel / Q000494:pharmacology; D010455:Peptides / Q000032:analysis / Q000502:physiology; D010590:Phalloidine / Q000494:pharmacology; D059828:Plant Cells; D018521:Plant Physiological Phenomena; D010940:Plant Proteins / Q000032:analysis / Q000502:physiology; D010944:Plants / Q000648:ultrastructure*; D018087:Plastids / Q000737:chemistry / Q000502:physiology / Q000648:ultrastructure*; D012399:Rotation
chemical_list: D010455:Peptides; D010940:Plant Proteins; D010590:Phalloidine; D015638:Cytochalasin D; D017239:Paclitaxel; D003078:Colchicine