A ground-based study for a shuttle BRIC experiment on gravity effects on gene expression.
A BRIC (Biological Research In a Canister) experiment to investigate the effects of reduced gravity at the molecular level using Arabidopsis has been initiated. In preparation for a space flight experiment, a series of ground-based studies were conducted. Results from these studies indicate that: 1) up to 20,000 seeds can be germinated on a 100 mm diameter Petri plate, 2) nylon membrane is the best surface for recovery of plant material after freezing, 3) depending on the age of the seedlings at the time of freezing, 20 to 40 g of tissue can be obtained from Petri plates that fit in a single canister; 4) tissue from one canister yields adequate amounts of RNA to perform differential display to isolate gravity-regulated genes. Our results indicate that the proposed BRIC experiment is feasible and can provide valuable information on the possible effects of microgravity on gene regulation.
Reddy
A S
AS
Biology Department, Center for Engineering Infrastructure and Sciences in Space, Colorado State University, Fort Collins 80523, USA.
Kao
Y L
YL
Mykles
D L
DL
Sadeh
W Z
WZ
Wheeler
R M
RM
D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development*; D005240:Feasibility Studies; D018506:Gene Expression Regulation, Plant; D018525:Germination / Q000502:physiology; D018471:Hypergravity; D018749:RNA, Plant; D012639:Seeds; D013026:Space Flight; D014732:Vibration; D014893:Weightlessness
D018749:RNA, Plant