title: Collateral growth and angiogenesis around cortical stroke.
abstract: OBJECTIVE
  We tested the hypothesis that there are significant long-term local vascular changes after ministroke that could form a basis for functional recovery.
  
  
  METHODS
  A 6- to 8-mm cranial window was opened over the barrel cortex, which was identified by an intrinsic optical signal during mechanical stimulation of the whiskers in anesthetized female Wistar rats. Branches of the middle cerebral artery (MCA) to this region were ligated. Fluorescein isothiocyanate (FITC) transits were recorded by videomicroscopy in each rat just before, immediately after, and 30 days after ligation. Changes in surface vessels and parenchymal perfusion were measured. In similarly prepared rats, angiogenesis was identified by 5-bromo-2-deoxyuridine labeling and immunohistochemistry for the integrin family member alpha(v)beta(3).
  
  
  RESULTS
  The intrinsic optical signal disappeared immediately after MCA ligations. FITC injection just after ligation demonstrated 3 concentric regions: 1 region of unchanged perfusion, surrounding 1 region of reduced perfusion (the ischemic border) surrounding a central core with little observable perfusion. At 30 days, the following had taken place: (1) diameters and lengths of surface collaterals in the ischemic border had grown significantly, but no new surface vessels were detected, (2) FITC entered occluded MCA segments, (3) arteriocapillary latencies in the ischemic border were shortened compared with latencies just after ligation, and (4) small infarcts were virtually identical to the poorly perfused core. Angiogenesis was confined to the ischemic border.
  
  
  CONCLUSIONS
  Arteriolar collateral growth and new capillaries support restored perfusion in the ischemic border after ministroke and could support long-term functional recovery.
authors.affiliation: Department of Neurology, Washington University School of Medicine, St. Louis, MO, USA. weil@neuro.wustl.edu
authors.forename: L
authors.initials: L
authors.lastname: Wei
authors.forename: J P
authors.initials: JP
authors.lastname: Erinjeri
authors.forename: C M
authors.initials: CM
authors.lastname: Rovainen
authors.forename: T A
authors.initials: TA
authors.lastname: Woolsey
mesh_terms: D000818:Animals; D001160:Arterioles / Q000000981:diagnostic imaging / Q000473:pathology; D002545:Brain Ischemia / Q000473:pathology; D001973:Bromodeoxyuridine; D002533:Cerebral Angiography; D002560:Cerebrovascular Circulation; D003097:Collateral Circulation; D004730:Endothelium, Vascular / Q000378:metabolism; D005260:Female; D005456:Fluorescent Dyes; D008026:Ligation; D008833:Microcirculation / Q000000981:diagnostic imaging / Q000378:metabolism / Q000473:pathology; D018715:Microscopy, Video / Q000379:methods; D020768:Middle Cerebral Artery; D018919:Neovascularization, Physiologic; D051381:Rats; D017208:Rats, Wistar; D019030:Receptors, Vitronectin / Q000378:metabolism; D013003:Somatosensory Cortex / Q000098:blood supply / Q000378:metabolism / Q000473:pathology*; D020521:Stroke / Q000473:pathology*
chemical_list: D005456:Fluorescent Dyes; D019030:Receptors, Vitronectin; D001973:Bromodeoxyuridine