title: A molecular model of type I allergy: identification and characterization of a nonanaphylactic anti-human IgE antibody fragment that blocks the IgE-FcepsilonRI interaction and reacts with receptor-bound IgE.
abstract: BACKGROUND
  The IgE-mediated activation of effector cells and antigen-presenting cells through the high-affinity receptor for IgE (FcepsilonRI) represents a key pathomechanism in type I allergy and many forms of asthma.
  
  
  OBJECTIVE
  We sought to establish an in vitro molecular model for the interaction of human FcepsilonRI, IgE, and the corresponding allergen and to identify monoclonal anti-human IgE antibodies with a therapeutic profile different from previously established anti-IgE antibodies.
  
  
  METHODS
  Human FcepsilonRI alpha chain, a human monoclonal allergen-specific IgE antibody (chimeric Bip 1), and the corresponding allergen, the major birch pollen allergen Bet v 1, were produced as recombinant proteins and analyzed by means of circular dichroism and native overlays, respectively. Using this molecular model, as well as negative stain immunoelectron microscopic analysis, and in vitro cultivated human basophils, we characterized mouse anti-human IgE antibodies.
  
  
  RESULTS
  We established a molecular model for the interaction of human IgE with FcepsilonRI. Using this molecular model, we identified a nonanaphylactic anti-human IgE antibody fragment (Fab12), which blocked the IgE-FcepsilonRI interaction and reacted with effector cell-bound IgE.
  
  
  CONCLUSIONS
  Fab12 represents a candidate molecule for therapy of atopy and asthma because it can be used for the depletion of circulating IgE antibodies, as well as for the depletion of IgE-bearing cells.
authors.affiliation: Department of Pathophysiology, AKH, University of Vienna, Vienna, Austria.
authors.forename: S
authors.initials: S
authors.lastname: Laffer
authors.forename: E
authors.initials: E
authors.lastname: Hogbom
authors.forename: K H
authors.initials: KH
authors.lastname: Roux
authors.forename: W R
authors.initials: WR
authors.lastname: Sperr
authors.forename: P
authors.initials: P
authors.lastname: Valent
authors.forename: H C
authors.initials: HC
authors.lastname: Bankl
authors.forename: L
authors.initials: L
authors.lastname: Vangelista
authors.forename: F
authors.initials: F
authors.lastname: Kricek
authors.forename: D
authors.initials: D
authors.lastname: Kraft
authors.forename: H
authors.initials: H
authors.lastname: Grönlund
authors.forename: R
authors.initials: R
authors.lastname: Valenta
mesh_terms: D000485:Allergens / Q000276:immunology; D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D000936:Antigen-Antibody Complex / Q000648:ultrastructure; D052179:Antigens, Plant; D001491:Basophils / Q000276:immunology; D006636:Histamine Release; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology*; D007073:Immunoglobulin E / Q000276:immunology*; D007141:Immunoglobulin Fc Fragments / Q000276:immunology*; D051379:Mice; D010940:Plant Proteins / Q000276:immunology; D011485:Protein Binding; D017455:Receptors, IgE / Q000276:immunology*
chemical_list: D000485:Allergens; D000888:Antibodies, Anti-Idiotypic; D000936:Antigen-Antibody Complex; D052179:Antigens, Plant; D007141:Immunoglobulin Fc Fragments; D010940:Plant Proteins; D017455:Receptors, IgE; C026408:anti-IgE antibodies; C061312:Bet v 1 allergen, Betula; D007073:Immunoglobulin E