title: CNA42 monoclonal antibody identifies FDC as PrPsc accumulating cells in the spleen of scrapie affected sheep.
abstract: Natural scrapie, new variant Creutzfeldt-Jakob disease and murine experimental transmissible spongiform encephalopathies (TSE) are fatal neurodegenerative disorders. The agent responsible for these diseases is closely related to PrPsc, an abnormal isoform of the cellular prion protein. Before reaching the brain, it invades and replicates in lymphoid organs such as spleen, tonsils and lymph nodes. Follicular dendritic cells (FDC) may support the prion replication in lymphoid tissues of sheep as shown in murine models infected with scrapie. In sheep, specific antibodies recognising FDC are lacking. The CNA42 mAb, directed against human FDC was used to identify these cells in sheep spleen. As well as showing that the pre-treatments needed for immunohistochemical detection of PrPsc did not prevent labelling by the CNA42 mAb, accumulation of PrPsc in FDC of spleens of scrapie affected sheep was demonstrated using a double immunolabelling strategy. Thus, the CNA42 antibody represents a suitable tool to identify FDC and investigate their role in natural sheep scrapie.
authors.affiliation: Agence Française de Sécurité Sanitaire des Aliments (AFSSA), Unité de Virologie, ATNC, 31 Avenue Tony Garnier, 69364 Cedex 07, Lyon, France. s.lezmi@caramail.com
authors.forename: S
authors.initials: S
authors.lastname: Lezmi
authors.forename: A
authors.initials: A
authors.lastname: Bencsik
authors.forename: T
authors.initials: T
authors.lastname: Baron
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D020566:Dendritic Cells, Follicular / Q000378:metabolism*; D007150:Immunohistochemistry; D018620:PrPSc Proteins / Q000378:metabolism*; D012608:Scrapie / Q000378:metabolism*; D012756:Sheep; D013154:Spleen / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D018620:PrPSc Proteins