Pulmonary granuloma caused by Pseudomonas andersonii sp nov.
Pulmonary granuloma is a common lesion for which gram-negative bacteria are rarely implicated as a cause. Hence, most physicians are unaware of this etiology. We isolated a gram-negative bacterium from a surgically resected pulmonary granuloma in a 42-year-old, nonimmunocompromised woman. Within the necrotizing granuloma, numerous organisms also were demonstrated by Gram stain, suggesting a cause-disease relationship. Characterization of the bacterium by sequence analysis of the 16S ribosomal gene, cellular fatty acid profiling, and microbiologic studies revealed a novel bacterium with a close relationship to Pseudomonas. We propose a new species for the bacterium, Pseudomonas andersonii. These results suggest that the differential diagnosis of a lung granuloma also should include this gram-negative bacterium as a potential causative agent, in addition to the more common infections caused by acid-fast bacilli and fungi. This bacterium was shown to be susceptible to most antibiotics that are active against gram-negative bacteria.
Han
X Y
XY
Dept of Laboratory Medicine, Box 84, University of Texas M.D. Anderson Cancer Center, 1515 Holcombe Blvd, Houston, TX 77030, USA.
Pham
A S
AS
Nguyen
K U
KU
Smythe
W R
WR
Ordonez
N G
NG
Jacobson
K L
KL
Tarrand
J J
JJ
D000328:Adult; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D017931:DNA Primers / Q000737:chemistry; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D005260:Female; D006099:Granuloma / Q000382:microbiology* / Q000473:pathology / Q000601:surgery; D006801:Humans; D008171:Lung Diseases / Q000382:microbiology* / Q000473:pathology / Q000601:surgery; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction; D011549:Pseudomonas / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification* / Q000648:ultrastructure; D011552:Pseudomonas Infections / Q000150:complications* / Q000473:pathology / Q000601:surgery; D014057:Tomography, X-Ray Computed
D000900:Anti-Bacterial Agents; D017931:DNA Primers; D004269:DNA, Bacterial; D004275:DNA, Ribosomal