Altered gravity modulates 5-lipoxygenase in human erythroleukemia K562 cells.
Mammalian lipoxygenases catalyse the first committed step in the so-called "arachidonate cascade", leading to the production of potent bioactive molecules, such as leukotrienes, lipoxins and hepoxilins. Leukotrienes interact with G protein-couple receptors involved in neuronal plasticity and T lymphocyte activation, lipoxins activate leukocytes, hepoxilines control the insulin release and stimulate the phospholipase C. Lipoxygenase (linoleate:oxygen oxidoreductase; E.C. 1.13.11.34; 5-LOX) are responsible for lymphocyte maturation and programmed death (apoptosis) of neuronal cells. Therefore, 5-LOX might be Space relevant, because among the most striking effects of Space enviroment are indeed those on T lymphocyte activation, neuronal cell growth and suspectedly apoptosis.  In this study, the possible effects of the force of gravity on the activity and expression of 5-LOX have been investigated by subjecting human erythroleukemia K562 cells to simulated hypogravity or hypergravity.
Maccarrone
M
M
Department of Experimental Medicine and Biochemical Sciences, University of Rome "Tor Vergata", Italy.
Putti
S
S
Finazzi Agro
A
A
D001094:Arachidonate 5-Lipoxygenase / Q000378:metabolism*; D002498:Centrifugation; D005786:Gene Expression Regulation; D018470:Gravity, Altered; D006801:Humans; D018471:Hypergravity; D020014:K562 Cells / Q000378:metabolism*; D015227:Lipid Peroxidation; D008213:Lymphocyte Activation / Q000502:physiology; D008563:Membrane Lipids / Q000378:metabolism; D012399:Rotation; D018474:Weightlessness Simulation
D008563:Membrane Lipids; D001094:Arachidonate 5-Lipoxygenase