A new facile method for spin-labeling of oligonucleotides.
A new facile method for spin-labeling suitable for DNA and RNA oligonucleotides is presented. The nitroxide 3-ethenyl-2,2,5,5-tetramethyl-pyrrolin-1-yloxy was directly introduced during automated solid-phase synthesis by a Pd(0) cross coupling reaction. The main advantages of this procedure are the small amount of spin-label needed for the derivatisation of the oligonucleotide and the high coupling efficiency on the solid phase.
Strube
T
T
Institut für Organische Chemie, Johann Wolfgang Goethe-Universität, Marie Curie Strasse 11, D-60439 Frankfurt am Main, Germany.
Schiemann
O
O
MacMillan
F
F
Prisner
T
T
Engels
J W
JW
D004247:DNA / Q000138:chemical synthesis / Q000737:chemistry; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D012313:RNA / Q000138:chemical synthesis / Q000737:chemistry; D013113:Spin Labels / Q000138:chemical synthesis*
D009841:Oligonucleotides; D013113:Spin Labels; D012313:RNA; D004247:DNA