Labeling during cleavage (LDC), a new labeling approach for RNA.
A new and efficient strategy for labeling of RNA sequences prior to their hybridization on high density DNA chip has been developed. Our approach which combines the fragmentation and the labeling is based on the reactivity of the 3'-phosphate of cleaved RNA fragments with a fluorescent molecule bearing aromatic bromomethyl function.
Monnot
V
V
BioMérieux, sa-Department of Molecular Biology, Chemin de l'Orme 69280-Marcy l'Etoile, France.
Tora
C
C
Lopez
S
S
Menou
L
L
Laayoun
A
A
D012321:DNA-Directed RNA Polymerases / Q000737:chemistry / Q000235:genetics; D005456:Fluorescent Dyes / Q000737:chemistry; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D012313:RNA / Q000737:chemistry*; D012336:RNA, Ribosomal, 16S / Q000737:chemistry / Q000235:genetics; D014764:Viral Proteins
D005456:Fluorescent Dyes; D012336:RNA, Ribosomal, 16S; D014764:Viral Proteins; D012313:RNA; C062210:bacteriophage T7 RNA polymerase; D012321:DNA-Directed RNA Polymerases