title: Ultrahigh-resolution colocalization of spectrally separable point-like fluorescent probes.
abstract: An ultrahigh-resolution colocalization method based on the simultaneous acquisition and analysis of spectrally separated images of the excitation point-spread function of point-like fluorescent probes is reviewed. It is shown that molecular distances can be measured with accuracy better than 10 nm using conventional far-field optics. A detailed account of the methodology, theoretical considerations, signal processing, and data fitting algorithms is given.
authors.affiliation: Material Sciences Division and Physical Biosciences Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, California 94720, USA.
authors.forename: X
authors.initials: X
authors.lastname: Michalet
authors.forename: T D
authors.initials: TD
authors.lastname: Lacoste
authors.forename: S
authors.initials: S
authors.lastname: Weiss
mesh_terms: D005456:Fluorescent Dyes / Q000302:isolation & purification*; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*; D017785:Photons; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods*; D014159:Transducers
chemical_list: D005456:Fluorescent Dyes