title: Selection of deoxyribozyme ligases that catalyze the formation of an unnatural internucleotide linkage.
abstract: The chemical ligation of DNA molecules can be mediated by terminal phosphorothioate displacement of a 5' iodine. We have selected deoxyribozymes that can catalyze the formation of such phosphorothioester internucleotide linkages. The selected deoxyribozymes enhance the rate of ligation in part through the provision of a template that aligns the ligation junction and do not appear to require metal ions for catalysis.
authors.affiliation: Department of Chemistry and Biochemistry, Institute for Cellular and Molecular Biology, University of Texas at Austin, 78712, USA.
authors.forename: M
authors.initials: M
authors.lastname: Levy
authors.forename: A D
authors.initials: AD
authors.lastname: Ellington
mesh_terms: D001483:Base Sequence; D002384:Catalysis; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D011088:DNA Ligases / Q000378:metabolism*; D021881:DNA, Catalytic / Q000378:metabolism*; D004277:DNA, Single-Stranded / Q000378:metabolism; D003854:Deoxyribonucleotides / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D007455:Iodine / Q000378:metabolism; D008670:Metals / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009690:Nucleic Acid Conformation; D017422:Sequence Analysis, DNA; D013329:Structure-Activity Relationship; D013379:Substrate Specificity; D013873:Thionucleotides / Q000378:metabolism
chemical_list: D021881:DNA, Catalytic; D004277:DNA, Single-Stranded; D003854:Deoxyribonucleotides; D008670:Metals; D013873:Thionucleotides; D004247:DNA; D007455:Iodine; D011088:DNA Ligases