title: Ion-exchange chromatography separates activities synthesizing and degrading fructose 2,6-bisphosphate from C3 and C4 leaves but not from rat liver.
abstract: Fructose-6-phosphate,2-kinase and fructose-2,6-bisphosphatase were separated on the basis of charge from leaves of C3 (spinach, lettuce, and pea) and C4 (sorghum and amaranthus) plants but not from rat liver--a tissue known to contain a bifunctional enzyme with both activities.  [2-32P]Fructose 2,6-bisphosphate binding experiments also suggest that the major forms of these activities reside on different proteins in leaves.
authors.affiliation: Division of Molecular Plant Biology, University of California, Berkeley 94720, USA.
authors.forename: F D
authors.initials: FD
authors.lastname: Macdonald
authors.forename: Q
authors.initials: Q
authors.lastname: Chou
authors.forename: B B
authors.initials: BB
authors.lastname: Buchanan
mesh_terms: D000818:Animals; D002852:Chromatography, Ion Exchange; D005260:Female; D005635:Fructosediphosphates / Q000096:biosynthesis / Q000378:metabolism*; D005636:Fructosephosphates / Q000096:biosynthesis / Q000378:metabolism; D018545:Lactuca; D008099:Liver / Q000737:chemistry / Q000201:enzymology / Q000378:metabolism*; D019684:Magnoliopsida; D008297:Male; D018532:Pisum sativum; D025481:Phosphofructokinase-2; D010744:Phosphoric Monoester Hydrolases / Q000032:analysis / Q000378:metabolism*; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000032:analysis / Q000378:metabolism*; D018515:Plant Leaves / Q000737:chemistry / Q000145:classification / Q000201:enzymology / Q000378:metabolism*; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism; D006109:Poaceae; D051381:Rats; D017207:Rats, Sprague-Dawley; D018724:Spinacia oleracea
chemical_list: D005635:Fructosediphosphates; D005636:Fructosephosphates; D010940:Plant Proteins; C027618:fructose-6-phosphate; C027652:fructose 2,6-diphosphate; D017853:Phosphotransferases (Alcohol Group Acceptor); D025481:Phosphofructokinase-2; D010744:Phosphoric Monoester Hydrolases