title: Synthesis of chimeric oligonucleotides containing internucleosidic phosphodiester and S-pivaloylthioethyl phosphotriester residues.
abstract: Novel oligonucleotide analogs that bear phosphodiester and bioreversible S-pivaloyl 2-mercaptoethyl (SPME) phosphate triester internucleosidic linkages are described. Their synthesis employs a novel methodology of oligonucleotide deprotection under mild, non-aqueous conditions.
authors.affiliation: Department of Medicinal Chemistry, Isis Pharmaceuticals, 2292 Faraday Ave., Carlsbad, California 92008, USA.
authors.forename: A P
authors.initials: AP
authors.lastname: Guzaev
authors.forename: B
authors.initials: B
authors.lastname: Bhat
authors.forename: G
authors.initials: G
authors.lastname: Balow
authors.forename: M
authors.initials: M
authors.lastname: Manoharan
mesh_terms: D016376:Oligonucleotides, Antisense / Q000138:chemical synthesis*; D010755:Organophosphates / Q000138:chemical synthesis* / Q000737:chemistry; D009943:Organophosphorus Compounds / Q000138:chemical synthesis / Q000737:chemistry; D009946:Organothiophosphorus Compounds / Q000737:chemistry; D010421:Pentanoic Acids / Q000737:chemistry
chemical_list: D016376:Oligonucleotides, Antisense; D010755:Organophosphates; D009943:Organophosphorus Compounds; D009946:Organothiophosphorus Compounds; D010421:Pentanoic Acids; C434331:phosphoramidite