title: A novel beta-glucosidase from the cell wall of maize (Zea mays L.): rapid purification and partial characterization.
abstract: Plants have a variety of glycosidic conjugates of hormones, defense compounds, and other molecules that are hydrolyzed by beta-glucosidases (beta-D-glucoside glucohydrolases, E.C. 3.2.1.21). Workers have reported several beta-glucosidases from maize (Zea mays L.; Poaceae), but have localized them mostly by indirect means. We have purified and partly characterized a 58-Ku beta-glucosidase from maize, which we conclude from a partial sequence analysis, from kinetic data, and from its localization is not identical to any of those already reported. A monoclonal antibody, mWP 19, binds this enzyme, and localizes it in the cell walls of maize coleoptiles. An earlier report showed that mWP19 inhibits peroxidase activity in crude cell wall extracts and can immunoprecipitate peroxidase activity from these extracts, yet purified preparations of the 58 Ku protein had little or no peroxidase activity. The level of sequence similarity between beta-glucosidases and peroxidases makes it unlikely that these enzymes share epitopes in common. Contrary to a previous conclusion, these results suggest that the enzyme recognized by mWP19 is not a peroxidase, but there is a wall peroxidase closely associated with the 58 Ku beta-glucosidase in crude preparations. Other workers also have co-purified distinct proteins with beta-glucosidases. We found no significant charge in the level of immunodetectable beta-glucosidase in mesocotyls or coleoptiles that precedes the red light-induced changes in the growth rate of these tissues.
authors.affiliation: Division of Biological Sciences, The University of Texas, Austin 78712, USA.
authors.forename: W P
authors.initials: WP
authors.lastname: Nematollahi
authors.forename: S J
authors.initials: SJ
authors.lastname: Roux
mesh_terms: D000911:Antibodies, Monoclonal; D002473:Cell Wall / Q000201:enzymology; D018548:Cotyledon / Q000737:chemistry / Q000166:cytology / Q000201:enzymology / Q000528:radiation effects; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D007527:Isoenzymes; D008027:Light; D008969:Molecular Sequence Data; D010544:Peroxidases / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D020539:Sequence Analysis, Protein; D003313:Zea mays / Q000737:chemistry / Q000166:cytology / Q000201:enzymology* / Q000528:radiation effects; D001617:beta-Glucosidase / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D007527:Isoenzymes; D010940:Plant Proteins; D010544:Peroxidases; D001617:beta-Glucosidase