Alpha-lactose monohydrate single crystals as hosts for matrix isolation of guest biopolymers.
Single crystals of alpha-lactose monohydrate show a remarkable tendency to include biopolymers, such as proteins, oligonucleotides and dextrans, within the growing lattice. Glycosylation increased the amount of protein contained within the crystals. The guest molecules were found only within the (010) growth sector of the hatchet shaped crystals, thereby binding preferentially to one of the seven developed crystal faces. The topographical features of the active surface are described.
Wang
H C
HC
Department of Chemistry, Purdue University, West Lafayette, IN 47907-1393, USA.
Kurimoto
M
M
Kahr
B
B
Chmielewski
J
J
D000818:Animals; D001360:Avidin / Q000737:chemistry; D001704:Biopolymers / Q000737:chemistry*; D003460:Crystallization; D003574:Cytochrome c Group / Q000737:chemistry; D045304:Cytochromes c; D003911:Dextrans / Q000737:chemistry; D019793:Fluorescein; D006031:Glycosylation; D006801:Humans; D007785:Lactose / Q000737:chemistry*; D037102:Lectins / Q000737:chemistry; D018625:Microscopy, Atomic Force; D008857:Microscopy, Interference; D008958:Models, Molecular; D009113:Muramidase / Q000737:chemistry; D009841:Oligonucleotides / Q000737:chemistry; D012260:Ribonucleases / Q000737:chemistry
D001704:Biopolymers; D003574:Cytochrome c Group; D003911:Dextrans; D037102:Lectins; D009841:Oligonucleotides; C050095:zinc cytochrome c; D001360:Avidin; D045304:Cytochromes c; D012260:Ribonucleases; D009113:Muramidase; D007785:Lactose; D019793:Fluorescein