title: Molecular strain typing of Campylobacter jejuni by pulsed-field gel electrophoresis in a single day.
abstract: Rapid molecular strain typing is critical for effective outbreak investigation and implementation of infection control measures. Pulsed-field gel electrophoresis is a highly discriminatory technique for Campylobacter jejuni, but generally requires 3-5 days. We describe a simplified protocol for pulsed-field gel electrophoresis that provides high quality typing of C. jejuni isolates in a single day.
authors.affiliation: VA Medical Center, and Department of Medicine Boston University School of Medicine, MA 02130, USA. smichaud@courrier.usherb.ca
authors.forename: S
authors.initials: S
authors.lastname: Michaud
authors.forename: R D
authors.initials: RD
authors.lastname: Arbeit
authors.forename: C
authors.initials: C
authors.lastname: Gaudreau
mesh_terms: D000328:Adult; D000368:Aged; D015373:Bacterial Typing Techniques; D002169:Campylobacter Infections / Q000453:epidemiology* / Q000382:microbiology; D016123:Campylobacter jejuni / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000032:analysis*; D016521:Electrophoresis, Gel, Pulsed-Field; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D017720:Molecular Epidemiology; D013997:Time Factors
chemical_list: D004269:DNA, Bacterial