Expression of cspH, encoding the cold shock protein in Salmonella enterica serovar Typhimurium UK-1.
Both Salmonella enterica serovar Typhimurium and Escherichia coli contain the cspH gene encoding CspH, one of the cold shock proteins (CSPs). In this study, we investigated the expression of cspH in S. enterica serovar Typhimurium and found that it was induced in response to a temperature downshift during exponential phase. The cspH promoter was activated at 37 degrees C, and its mRNA was more stable than the other csp mRNAs at 37 degrees C. Moreover, lacZ expression of the translational cspH-lacZ fusion was induced at that temperature. Interestingly, the cspH mRNA had a much shorter 5'-untranslated region than those in the other cold-shock-inducible genes, and the promoter sequence, which was only 55 bp, was sufficient for cspH expression. The 14-base downstream box located 12 bases downstream of the initiation codon of cspH mRNA was essential for its cold shock activation.
Kim
B H
BH
Graduate School of Biotechnology, Korea University, Seoul 136-701, Korea.
Bang
I S
IS
Lee
S Y
SY
Hong
S K
SK
Bang
S H
SH
Lee
I S
IS
Park
Y K
YK
D020121:5' Untranslated Regions; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D003080:Cold Temperature; D015964:Gene Expression Regulation, Bacterial; D006360:Heat-Shock Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000378:metabolism; D012486:Salmonella typhimurium / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D017422:Sequence Analysis, DNA; D013696:Temperature
D020121:5' Untranslated Regions; D001426:Bacterial Proteins; C436307:CspH protein, Salmonella; D006360:Heat-Shock Proteins; D012333:RNA, Messenger