In vitro selection of catalytic RNAs.
In vitro selection techniques are poised to allow a rapid expansion of the study of catalysis by RNA enzymes (ribozymes).  This truly molecular version of genetics has already been applied to the study of the structures of known ribozymes and to the tailoring of their catalytic activity to meet specific requirements of substrate specificity or reaction conditions.  During the past year, in vitro selection has been successfully used to isolate novel RNA catalysts from random sequence pools.
Chapman
K B
KB
Massachusetts General Hospital, Boston, USA.
Szostak
J W
JW
D002384:Catalysis; D004722:Endoribonucleases / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D019143:Evolution, Molecular; D005821:Genetic Techniques; D007438:Introns / Q000235:genetics*; D012329:RNA, Bacterial / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D016337:RNA, Catalytic / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D043262:Ribonuclease P; D012261:Ribonucleoproteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism
D012329:RNA, Bacterial; D016337:RNA, Catalytic; D012261:Ribonucleoproteins; D004722:Endoribonucleases; D043262:Ribonuclease P