title: Expression of the Pasteurella multocida ompH gene is negatively regulated by the Fur protein.
abstract: The fur gene of Pasteurella multocida has been cloned by complementation of an Escherichia coli fur mutant. The P. multocida fur gene, which encodes a predicted protein of 147 amino acids, displaying the highest identity (89%) with the same protein of Haemophilus influenzae, is negatively regulated by its own product. By construction of a P. multocida fur mutant, it has been demonstrated that the ompH gene, encoding a major structural protein of the outer membrane, presenting high antigenicity power, is negatively regulated by iron and glucose. Furthermore, wild-type and fur-defective cells of P. multocida show the same level of virulence.
authors.affiliation: Department of Genetics and Microbiology, Universitat Autònoma de Barcelona (UAB), Bellaterra, 08193 Barcelona, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Bosch
authors.forename: R
authors.initials: R
authors.lastname: Tarragó
authors.forename: M E
authors.initials: ME
authors.lastname: Garrido
authors.forename: S
authors.initials: S
authors.lastname: Campoy
authors.forename: A R
authors.initials: AR
authors.lastname: Fernández de Henestrosa
authors.forename: A M
authors.initials: AM
authors.lastname: Pérez de Rozas
authors.forename: I
authors.initials: I
authors.lastname: Badiola
authors.forename: J
authors.initials: J
authors.lastname: Barbé
mesh_terms: D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D015536:Down-Regulation; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D005947:Glucose / Q000494:pharmacology; D007501:Iron / Q000494:pharmacology; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D016979:Pasteurella multocida / Q000235:genetics* / Q000472:pathogenicity; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D014774:Virulence
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D012097:Repressor Proteins; C068477:ferric uptake regulating proteins, bacterial; C067128:ompH protein, bacteria; D007501:Iron; D005947:Glucose