Methods for short time series analysis of cell-based biosensor data.
This paper describes two approaches for sensing changes in spiking cells when only a limited amount of spike data is available, i.e., dynamically constructed local expansion rates and spike area distributions. The two methods were tested on time series from cultured neuron cells that exhibit spiking both autonomously and in the presence of periodic stimulation. Our tested hypothesis was that minute concentrations of toxins could affect the local statistics of the dynamics. Short data sets having relatively few spikes were generated from experiments on cells before and after being treated with a small concentration of channel blocker. In spontaneous spiking cells, local expansion rates show a sensitivity that correlates with channel concentration level, while stimulated cells show no such correlation. Spike area distributions on the other hand showed measurable differences between control and treated conditions for both types of spiking, and a much higher degree of sensitivity. Because these methods are based on analysis of short time series analysis, they might provide novel means for cell drug and toxin detection.
Schwartz
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IB
Naval Research Laboratory, Special Project in Nonlinear Science, Code 6700.3, Washington, DC 20375, USA. schwartz@nlschaos.nrl.navy.mil
Billings
L
L
Pancrazio
J J
JJ
Schnur
J M
JM
D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D015374:Biosensing Techniques / Q000706:statistics & numerical data*; D019256:Cadmium Chloride / Q000633:toxicity; D002460:Cell Line; D003627:Data Interpretation, Statistical; D051379:Mice; D009474:Neurons / Q000187:drug effects / Q000502:physiology; D009498:Neurotoxins / Q000633:toxicity; D018408:Patch-Clamp Techniques; D051381:Rats
D009498:Neurotoxins; D019256:Cadmium Chloride