Influence of microgravity on mitogen binding and cytoskeleton in Jurkat cells.
The effects of microgravity on Jurkat cells--a T-lymphoid cell line--was studied on a sounding rocket flight. An automated pre-programmed instrument permitted the injection of fluorescent labelled concanavalin A (Con A), culture medium and/or fixative at given times. An in-flight 1 g centrifuge allowed the comparison of the data obtained in microgravity with a 1 g control having the same history related to launch and re-entry. After flight, the cells fixed either at the onset of microgravity or after a or 12 minute incubation time with fluorescent concanavalin A were labelled for vimentin and actin and analysed by fluorescence microscopy. Binding of Con A to Jurkat cells is not influenced by microgravity, whereas patching of the Con A receptors is significantly lower. A significant higher number of cells show changes in the structure of vimentin in microgravity. Most evident is the appearance of large bundles, significantly increased in the microgravity samples. No changes are found in the structure of actin and in the colocalisation of actin on the inner side of the cell membrane with the Con A receptors after binding of the mitogen.
Sciola
L
L
Dep. Physiological, Biochemical and Cellular Sciences, University of Sassari, Italy.
Cogoli-Greuter
M
M
Cogoli
A
A
Spano
A
A
Pippia
P
P
D000199:Actins / Q000648:ultrastructure; D003208:Concanavalin A / Q000378:metabolism*; D003599:Cytoskeleton / Q000378:metabolism* / Q000502:physiology; D006801:Humans; D007152:Immunologic Capping; D019169:Jurkat Cells / Q000166:cytology / Q000378:metabolism* / Q000502:physiology; D008934:Mitogens / Q000378:metabolism; D011485:Protein Binding; D011952:Receptors, Concanavalin A / Q000378:metabolism*; D013026:Space Flight; D014746:Vimentin / Q000648:ultrastructure; D014893:Weightlessness
D000199:Actins; D008934:Mitogens; D011952:Receptors, Concanavalin A; D014746:Vimentin; D003208:Concanavalin A