Single nucleotide polymorphisms of the resistin (RSTN) gene.
Type 2 diabetes mellitus is a complex phenotype that is frequently associated with central obesity and insulin resistance. Recently, a protein named resistin, encoded by RSTN (OMIM #605565), was identified in adipose tissue. Serum resistin was elevated in obese and diabetic mice, and administration of resistin to normal mice was found to interfere with glucose tolerance and insulin action. Because of these functions, resistin is a candidate gene for diabetes and obesity. Through the use of DNA sequencing, we thus developed amplification primers for rapid screening of the RSTN gene that encodes resistin. No putative mutations were found, but two noncoding single-nucleotide polymorphisms (SNPs) were identified, and these were found to vary in frequency across various ethnic groups. The identification of amplification primers and SNPs provides tools to investigate resistin for association with other phenotypes.
Cao
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Blackburn Cardiovascular Genetics Laboratory, John P. Robarts Research Institute, London ON, Canada.
Hegele
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RA
D001483:Base Sequence; D002170:Canada; D017931:DNA Primers; D003920:Diabetes Mellitus / Q000235:genetics; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics*; D005006:Ethnicity / Q000235:genetics; D005787:Gene Frequency; D006729:Hormones, Ectopic / Q000235:genetics*; D006801:Humans; D007333:Insulin Resistance / Q000235:genetics; D036341:Intercellular Signaling Peptides and Proteins; D009765:Obesity; D020641:Polymorphism, Single Nucleotide; D044469:Racial Groups / Q000235:genetics; D052243:Resistin
D017931:DNA Primers; D006729:Hormones, Ectopic; D036341:Intercellular Signaling Peptides and Proteins; C499624:RETN protein, human; C486574:RETNLB protein, human; D052243:Resistin