title: Salivary cytomegalovirus (CMV) shedding, glycoprotein B genotype distribution, and CMV disease in human immunodeficiency virus-seropositive patients.
abstract: To assess the frequency of shedding of cytomegalovirus (CMV) in saliva, the distribution of CMV glycoprotein B (gB) genotypes, and the occurrence of CMV diseases, we screened 98 human immunodeficiency virus (HIV)-seropositive patients without CMV disease. CMV was detected by culture more frequently in saliva (45 [46%] of 98 patients) than in blood (7 [7.5%] of 93) and was associated with CD4 cell counts <100 cells/mm3 (P=.013). CMV in the saliva of 37 patients was successfully genotyped. Three patients (8%) were infected by a gB1 strain, 26 (70%) by a gB2 strain, 2 (5.5%) by a gB3 strain, 1 (3%) by a gB4 strain, and 5 (13.5%) by mixed gB strains. Thirteen patients developed CMV disease after a mean period of 143+/-112 days; at inclusion, 9 (69%) had salivary CMV shedding and 2 had CMV viremia. CMV salivary shedding (P=.043), low CD4+ cell count (P=.041), and CMV viremia (P=.011) were associated with occurrence of CMV disease.
authors.affiliation: Department of Virology, Bichat-Claude Bernard Hospital, Paris, France.
authors.forename: N
authors.initials: N
authors.lastname: Fidouh-Houhou
authors.forename: X
authors.initials: X
authors.lastname: Duval
authors.forename: F
authors.initials: F
authors.lastname: Bissuel
authors.forename: V
authors.initials: V
authors.lastname: Bourbonneux
authors.forename: P
authors.initials: P
authors.lastname: Flandre
authors.forename: J L
authors.initials: JL
authors.lastname: Ecobichon
authors.forename: M C
authors.initials: MC
authors.lastname: Jordan
authors.forename: J L
authors.initials: JL
authors.lastname: Vildé
authors.forename: F
authors.initials: F
authors.lastname: Brun-Vézinet
authors.forename: C
authors.initials: C
authors.lastname: Leport
mesh_terms: D017088:AIDS-Related Opportunistic Infections / Q000821:virology*; D003587:Cytomegalovirus / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D003586:Cytomegalovirus Infections / Q000097:blood / Q000821:virology*; D005260:Female; D015658:HIV Infections / Q000821:virology*; D006801:Humans; D008297:Male; D012463:Saliva / Q000821:virology*; D014759:Viral Envelope Proteins / Q000235:genetics*; D014766:Viremia / Q000821:virology; D014776:Virus Cultivation; D017201:Virus Shedding
chemical_list: D014759:Viral Envelope Proteins; C040495:glycoprotein B, Simplexvirus