title: The sequence of Methanospirillum hungatei 23S rRNA confirms the specific relationship between the extreme halophiles and the Methanomicrobiales.
abstract: We have determined the sequence of the 23S rRNA from the methanogenic archaeon Methanospirillum hungatei.  This is the first such sequence from a member of the Methanomicrobiales.  Moreover, it brings additional evidence to bear on the possible specific relationship between this particular group of methanogens and the extreme halophiles.  Such evidence is critical in that several new (and relatively untested) methods of phylogenetic inference have lead to the controversial conclusion that the extreme halophiles are either not related to the archaea, or are only peripherally so.  Analysis of the Methanospirillum hungatei 23S rRNA sequence shows the Methanomicrobiales are indeed a sister group of the extreme halophiles, further strengthening the conclusions reached from analysis of 16S rRNA sequences.
authors.affiliation: Department of Microbiology, University of Illinois, Urbana 61801, USA.
authors.forename: S
authors.initials: S
authors.lastname: Burggraf
authors.forename: A
authors.initials: A
authors.lastname: Ching
authors.forename: K O
authors.initials: KO
authors.lastname: Stetter
authors.forename: C R
authors.initials: CR
authors.lastname: Woese
mesh_terms: D001105:Archaea / Q000145:classification* / Q000235:genetics; D001483:Base Sequence; D005075:Biological Evolution; D016931:Halobacteriales / Q000145:classification* / Q000235:genetics; D017018:Methanomicrobiales / Q000145:classification* / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D012329:RNA, Bacterial / Q000145:classification / Q000235:genetics*; D012338:RNA, Ribosomal, 23S / Q000145:classification / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
chemical_list: D012329:RNA, Bacterial; D012338:RNA, Ribosomal, 23S