A single-stranded telomere binding protein in the nematode Caenorhabditis elegans.
We identified and characterized a protein (STB-1) from the nuclear extract of Caenorhabditis elegans that specifically binds single-stranded telomere DNA sequences, but not the corresponding RNA sequences. STB-1 binding activity is specific to the nematode telomere, but not to the human or plant telomere. STB-1 requires the core nucleotides of GCTTAGG and three spacer nucleotides in front of them for binding. While any single nucleotide change in the core sequence abolishes binding, the spacer nucleotides tolerate substitution. STB-1 was determined to be a basic protein of 45 kDa by Southwestern analyses. STB-1 forms a stable complex with DNA once bound to the telomere.
Yi
S Y
SY
Department of Biology, Yonsei University, 134 Shinchon-dong, Seodaemun-ku, Seoul 120-749, South Korea.
Joeng
K S
KS
Kweon
J U
JU
Cho
J W
JW
Chung
I K
IK
Lee
J
J
D000818:Animals; D015139:Blotting, Southern; D015153:Blotting, Western; D017173:Caenorhabditis elegans / Q000737:chemistry*; D002467:Cell Nucleus / Q000737:chemistry / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism* / Q000502:physiology*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009687:Nuclear Proteins / Q000378:metabolism* / Q000502:physiology*; D012275:Oryza / Q000737:chemistry; D010710:Phosphates / Q000737:chemistry; D017680:Potassium Compounds / Q000737:chemistry; D011485:Protein Binding; D016615:Telomere / Q000378:metabolism*
D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D010710:Phosphates; D017680:Potassium Compounds; D004247:DNA; C013216:potassium phosphate