Aptamers that bind to the antibiotic moenomycin A.
Nuclease-resistant moenomycin-binding aptamers with dissociation constants in the range of 300 to 400 nM have been selected. Competition experiments have demonstrated that these aptamers recognize a disaccharide analogue of moenomycin. The results offer the opportunity of setting up a selective and sensitive assay for identifying moenomycin biosynthetic precursors.
Schürer
H
H
Universität Leipzig, Institut für Biochemie, Talstr. 33, D-04103, Leipzig, Germany.
Stembera
K
K
Knoll
D
D
Mayer
G
G
Blind
M
M
Förster
H H
HH
Famulok
M
M
Welzel
P
P
Hahn
U
U
D000900:Anti-Bacterial Agents / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology*; D001419:Bacteria / Q000254:growth & development; D001431:Bacteriological Techniques; D001455:Bambermycins / Q000737:chemistry / Q000494:pharmacology*; D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D020650:Combinatorial Chemistry Techniques; D004247:DNA / Q000096:biosynthesis; D004187:Disaccharides / Q000737:chemistry; D004352:Drug Resistance, Microbial; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009841:Oligonucleotides / Q000737:chemistry / Q000378:metabolism; D012313:RNA / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism*; D017423:Sequence Analysis, RNA
D000900:Anti-Bacterial Agents; D004187:Disaccharides; D009841:Oligonucleotides; D001455:Bambermycins; C437243:moenomycin A; D012313:RNA; D004247:DNA