The NMR conformation study of the complexes of deoxycytidine kinase (dCK) and 2'-deoxycytidine/2'-deoxyadenosine.
The structures of the bound 13C/2H double-labelled 2'(R/S), 5'(R/S)-2H2-1',2',3',4',5'-13C5-2'-deoxyadenosine and the corresponding 2'-deoxycytidine moieties in the complexes with human deoxycytidine kinase (dCK) have been characterized for the first time by the solution NMR spectroscopy, using Transferred Dipole-Dipole Cross-correlated Relaxation and Transferred nOe experiments. It has been shown that the ligand adopts a South-type sugar conformation when bound to dCK.
Maltseva
T
T
Department of Bioorganic Chemistry, Box 581, Biomedical Center, University of Uppsala, S-751 23 Uppsala, Sweden.
Usova
E
E
Eriksson
S
S
Milecki
J
J
Földesi
A
A
Chattopadhayaya
J
J
D003839:Deoxyadenosines / Q000737:chemistry*; D003841:Deoxycytidine / Q000737:chemistry*; D003842:Deoxycytidine Kinase / Q000737:chemistry*; D006801:Humans; D019906:Nuclear Magnetic Resonance, Biomolecular; D011487:Protein Conformation
D003839:Deoxyadenosines; D003841:Deoxycytidine; D003842:Deoxycytidine Kinase