title: Solid-phase synthesis of oligodeoxynucleotides containing 3'-S-phosphorothiolate linkages.
abstract: For the first time a fully automated procedure has been developed for the incorporation of a 3'-S-phosphorothiolate linkage into DNA, using phosphorothioamidite monomers. Coupling yields with either of the activators 5-ethylthiotetrazole or 4,5-dicyanoimidazole were in the range of 80-90%. Coupling yields were equally good when performed on either a 0.2 or 1 mumole reaction column, thus facilitating large scale synthesis.
authors.affiliation: Department of Chemistry, University of Liverpool, Crown Street, Liverpool L69 7ZD, United Kingdom.
authors.forename: K J
authors.initials: KJ
authors.lastname: Fettes
authors.forename: I
authors.initials: I
authors.lastname: O'Neil
authors.forename: S M
authors.initials: SM
authors.lastname: Roberts
authors.forename: R
authors.initials: R
authors.lastname: Cosstick
mesh_terms: D009841:Oligonucleotides / Q000138:chemical synthesis*; D009946:Organothiophosphorus Compounds / Q000138:chemical synthesis*; D013873:Thionucleotides / Q000138:chemical synthesis*; D013936:Thymidine / Q000031:analogs & derivatives
chemical_list: D009841:Oligonucleotides; D009946:Organothiophosphorus Compounds; D013873:Thionucleotides; D013936:Thymidine