Isoprenoid biosynthesis. Metabolite profiling of peppermint oil gland secretory cells and application to herbicide target analysis.
Two independent pathways operate in plants for the synthesis of isopentenyl diphosphate and dimethylallyl diphosphate, the central intermediates in the biosynthesis of all isoprenoids. The mevalonate pathway is present in the cytosol, whereas the recently discovered mevalonate-independent pathway is localized to plastids. We have used isolated peppermint (Mentha piperita) oil gland secretory cells as an experimental model system to study the effects of the herbicides fosmidomycin, phosphonothrixin, methyl viologen, benzyl viologen, clomazone, 2-(dimethylamino)ethyl diphosphate, alendronate, and pamidronate on the pools of metabolites related to monoterpene biosynthesis via the mevalonate-independent pathway. A newly developed isolation protocol for polar metabolites together with an improved separation and detection method based on liquid chromatography-mass spectrometry have allowed assessment of the enzyme targets for a number of these herbicides.
Lange
B M
BM
Institute of Biological Chemistry, Washington State University, Pullman, Washington 99164-6340, USA.
Ketchum
R E
RE
Croteau
R B
RB
D001665:Binding Sites; D002250:Carbon Radioisotopes; D002853:Chromatography, Liquid; D003600:Cytosol / Q000378:metabolism; D045782:Hemiterpenes; D006540:Herbicides / Q000494:pharmacology*; D019686:Lamiaceae / Q000187:drug effects / Q000378:metabolism*; D013058:Mass Spectrometry; D036142:Mentha piperita; D008798:Mevalonic Acid / Q000378:metabolism*; D009943:Organophosphorus Compounds / Q000378:metabolism*; D010938:Plant Oils; D018514:Plant Structures / Q000187:drug effects / Q000378:metabolism; D018087:Plastids / Q000378:metabolism; D019289:Pyruvic Acid / Q000494:pharmacology; D013729:Terpenes / Q000378:metabolism*
D002250:Carbon Radioisotopes; D045782:Hemiterpenes; D006540:Herbicides; D009943:Organophosphorus Compounds; D010938:Plant Oils; D013729:Terpenes; C004809:isopentenyl pyrophosphate; D019289:Pyruvic Acid; C015424:peppermint oil; D008798:Mevalonic Acid