New approaches towards fluorescence labelling of messenger RNA transcripts.
Fluorescence labelling of nucleic acids is being used for a wide range of biological applications. The performance of these techniques is dependent on fluorochrome labels with a high sensitivity and high resistance to photobleaching. Indo-cyanine dyes such as Cy3, Cy5 or Cy7 have been found to fulfill these requirements. This study describes several different RNA labelling techniques allowing for a Cy5 based detection of mRNA transcripts.
Garnier
A
A
Novartis Pharma AG, CH-4002 Basel, Switzerland.
Hüsken
D
D
Weiler
J
J
D002232:Carbocyanines / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D012333:RNA, Messenger / Q000737:chemistry*
D002232:Carbocyanines; D005456:Fluorescent Dyes; D012333:RNA, Messenger; C085321:cyanine dye 5