title: Chemiluminescence assay of the antioxidant state in patients with atherosclerosis.
abstract: We evaluated antioxidant state of 62 patients with coronary heart disease and 47 patients with obliterating atherosclerosis of lower extremities by peroxide-depend chemiluminescence and inhibition of azo-initiated chemiluminescence and hydrogen peroxide-hemoglobin-luminol chemiluminescence system. The flash amplitude of peroxide-dependent chemiluminescence in the plasma from patients was 32% below the control. Antioxidant activity of the plasma from patients was higher than in healthy individuals by 33 and 27% depending on the type of free radical-generating systems. The increase in antioxidant activity was most pronounced in patients with combined pathology: coronary heart disease complicated by obliterating atherosclerosis. These results explain the decrease in peroxide-dependent chemiluminescence of the plasma and whole blood in patients with atherosclerosis compared to that in healthy individuals.
authors.affiliation: Institute of Physicochemical Medicine, Russian Ministry of Health, Moscow.
authors.forename: O A
authors.initials: OA
authors.lastname: Azizova
authors.forename: A P
authors.initials: AP
authors.lastname: Piryazev
authors.forename: M P
authors.initials: MP
authors.lastname: Sherstnev
authors.forename: S V
authors.initials: SV
authors.lastname: Drinitsina
authors.forename: Y M
authors.initials: YM
authors.lastname: Lopukhin
mesh_terms: D000975:Antioxidants / Q000378:metabolism* / Q000494:pharmacology*; D001161:Arteriosclerosis / Q000097:blood* / Q000378:metabolism*; D016022:Case-Control Studies; D018450:Disease Progression; D005609:Free Radicals; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008163:Luminescent Measurements
chemical_list: D000975:Antioxidants; D005609:Free Radicals; D006861:Hydrogen Peroxide