Effect of oral calcium and calcium + fluoride treatments on mouse bone properties during suspension.
The bone effects of oral dosages of calcium chloride with or without supplementary sodium fluoride were assessed in antiorthostatically suspended mice.  Two calcium dosages were used to replace half (3.1 mM) or all(6.3 mM) of the dietary calcium lost due to reduced food intake by the suspended mice.  Two groups of 6.3 mM CaCl2-treated mice were additionally treated with 0.25 or 2.5 mM NaF.  The results indicate that supplementation of the mouse drinking water with calcium salts prevents bone changes induced by short-term suspension, while calcium salts in combination with fluoride are less effective as fluoride dosage increases.  However, the calcium supplements change the relationship between the femur mechanical properties and the mineral composition of the bone.  Because of this, it appears that oral calcium supplements are effective through a mechanism other than simple dietary supplementation and may indicate a dependence of bone consistency on systemic and local fluid conditions.
Simske
S J
SJ
BioServe Space Technologies, University of Colorado, Boulder 80309-0429.
Luttges
M W
MW
Allen
K A
KA
D000818:Animals; D001696:Biomechanical Phenomena; D015519:Bone Density / Q000187:drug effects*; D001851:Bone Diseases, Metabolic / Q000188:drug therapy / Q000209:etiology / Q000517:prevention & control; D001862:Bone Resorption / Q000188:drug therapy* / Q000209:etiology / Q000517:prevention & control; D001842:Bone and Bones / Q000033:anatomy & histology / Q000187:drug effects / Q000502:physiology; D002113:Calcification, Physiologic / Q000187:drug effects*; D002122:Calcium Chloride / Q000494:pharmacology* / Q000627:therapeutic use; D004326:Drinking; D004435:Eating; D005260:Female; D006614:Hindlimb; D007103:Immobilization / Q000009:adverse effects*; D051379:Mice; D012969:Sodium Fluoride / Q000494:pharmacology* / Q000627:therapeutic use; D018474:Weightlessness Simulation
D012969:Sodium Fluoride; D002122:Calcium Chloride