Epitope mapping of neutralizing botulinum neurotoxin A antibodies by phage display.
Single-chain antibodies neutralize activity and bind nonoverlapping epitopes of botulinum A neurotoxin. Two phage display epitope libraries were constructed from the 1.3 kb of binding domain cDNA. The minimal epitopes selected against the single-chain Fv-Fc antibodies correspond to conformational epitopes with amino acid residues 1115 to 1223 (S25), 1131 to 1264 (3D12), and 889 to 1294 (C25).
Mullaney
B P
BP
Department of Laboratory Medicine, University of California at San Francisco, San Francisco, California 94143, USA. mullaney@cc.ucsf.edu
Pallavicini
M G
MG
Marks
J D
JD
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology*; D019274:Botulinum Toxins, Type A / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D003014:Clostridium botulinum / Q000276:immunology*; D018604:Epitope Mapping / Q000379:methods; D018985:Epitopes, B-Lymphocyte / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007128:Immunoglobulin Fragments / Q000276:immunology*; D007135:Immunoglobulin Variable Region / Q000276:immunology*; D051379:Mice; D008958:Models, Molecular; D009500:Neutralization Tests; D019151:Peptide Library; D017434:Protein Structure, Tertiary
D000907:Antibodies, Bacterial; D018985:Epitopes, B-Lymphocyte; D007128:Immunoglobulin Fragments; D007135:Immunoglobulin Variable Region; D019151:Peptide Library; C055966:immunoglobulin Fv; D019274:Botulinum Toxins, Type A