title: All-blood (miniplegia) versus dilute cardioplegia in experimental surgical revascularization of evolving infarction.
abstract: BACKGROUND
  The advantages of blood cardioplegia include the oxygen-carrying capacity, superior oncotic and buffering properties, and endogenous antioxidants contained in blood. However, the partial dilution of blood in 4:1 (blood:crystalloid) cardioplegic solutions may nullify these advantages and progressively dilute blood during continuous retrograde delivery. This study tested the hypothesis that all-blood (66:1) cardioplegia provides superior myocardial protection compared with dilute (4:1) cardioplegia delivered in a continuous retrograde modality during surgical reperfusion of evolving myocardial infarction.
  
  
  RESULTS
  After 60 minutes of left anterior descending coronary artery (LAD) occlusion, anesthetized canines were placed on cardiopulmonary bypass and randomized to either all-blood cardioplegia (AB group) or dilute blood cardioplegia (Dil group). After cross clamping, arrest was induced with 5 minutes of tepid (30 degrees C) antegrade potassium all-blood or dilute blood cardioplegia and maintained with tepid retrograde coronary sinus cardioplegia for a total of 1 hour. The LAD was released after 30 minutes of arrest, simulating revascularization. The cardioplegia hematocrit for the Dil group was lower than that for the AB group (7+/-1% versus 12+/-2%, P<0.05); at the end of bypass, systemic hematocrit was lower in the Dil group than in the Ab group (15+/-1% versus 20+/-1%, P<0.05). Infarct size (triphenyltetrazolium chloride staining) was comparable between the AB and Dil groups (29.6+/-2.9% versus 30.3+/-3.9% of area at risk), and there was no difference in area-at-risk myocardium systolic shortening (by sonomicrometry, -0.3+/-1% versus -0.4+/-1%). Tissue edema after bypass tended to be greater in the Dil group compared with the AB group in the heart (82+/-0% versus 81+/-1%), lung (79+/-1% versus 78+/-1%), liver (75+/-1% versus 74+/-0%), and skeletal muscle (76+/-1% versus 73+/-2%) and was significantly greater in the duodenum (80+/-1% versus 79+/-1%, P<0.05) and kidney (82+/-1% versus 79+/-1%, P<0.05). Postexperimental endothelial function (relaxation of acetylcholine) was impaired in LADs of the AB group versus the Dil group (59+/-6% versus 77+/-5%, P<0.05).
  
  
  CONCLUSIONS
  Both all-blood cardioplegia and dilute cardioplegia have disadvantages, but these do not have an impact on the pathogenesis of infarct size or recovery of regional contractile function.
authors.affiliation: Cardiothoracic Research Laboratory, Department of Surgery, Division of Cardiothoracic Surgery, Carlyle Fraser Heart Center, Emory University School of Medicine, Atlanta, GA, USA.
authors.forename: D A
authors.initials: DA
authors.lastname: Velez
authors.forename: C D
authors.initials: CD
authors.lastname: Morris
authors.forename: J M
authors.initials: JM
authors.lastname: Budde
authors.forename: S
authors.initials: S
authors.lastname: Muraki
authors.forename: R N
authors.initials: RN
authors.lastname: Otto
authors.forename: R A
authors.initials: RA
authors.lastname: Guyton
authors.forename: J
authors.initials: J
authors.lastname: Vinten-Johansen
mesh_terms: D000818:Animals; D001769:Blood; D001834:Body Water / Q000187:drug effects; D002314:Cardioplegic Solutions / Q000737:chemistry / Q000494:pharmacology*; D003331:Coronary Vessels / Q000187:drug effects / Q000473:pathology; D003402:Creatine Kinase / Q000097:blood; D004195:Disease Models, Animal; D018450:Disease Progression; D004285:Dogs; D004730:Endothelium, Vascular / Q000378:metabolism; D005260:Female; D006321:Heart / Q000187:drug effects; D006324:Heart Arrest, Induced / Q000379:methods*; D006439:Hemodynamics / Q000187:drug effects; D008297:Male; D009200:Myocardial Contraction / Q000187:drug effects; D009203:Myocardial Infarction / Q000201:enzymology / Q000473:pathology / Q000601:surgery*; D009204:Myocardial Revascularization / Q000379:methods*; D009206:Myocardium / Q000201:enzymology / Q000473:pathology; D009195:Peroxidase / Q000378:metabolism; D017680:Potassium Compounds / Q000737:chemistry / Q000494:pharmacology; D020127:Recovery of Function / Q000187:drug effects; D014662:Vasoconstrictor Agents / Q000494:pharmacology; D014664:Vasodilation / Q000187:drug effects; D014665:Vasodilator Agents / Q000494:pharmacology
chemical_list: D002314:Cardioplegic Solutions; D017680:Potassium Compounds; D014662:Vasoconstrictor Agents; D014665:Vasodilator Agents; C018369:potassium cardioplegic solution; D009195:Peroxidase; D003402:Creatine Kinase