Galanin-like immunoreactive neural elements in domestic ruminant pancreas.
The distribution and ontogeny of the galanin-like immunoreactive (Gal-IR) neural structures in the pancreas of cattle, sheep and goat were investigated immunohistochemically. The present study confirmed the previous findings on the immunolocalization of galanin both in the neural elements and endocrine cells of cattle, and reported for the first time its exclusive localization in the neural elements of sheep and goat. The frequency of Gal-IR nerve fibers and nerve cell bodies was high in cattle and low in sheep and goat. Their first detection was at the first fetal trimester in cattle and third trimester in sheep and goat. In cattle, a marked increase in the frequency of Gal-IR nerve fibers was observed from the third trimester to early neonatal stage followed by a decrease after three months postnatal. In contrast to the non-preferential distribution pattem in sheep, the Gal-IR nerve fibers in cattle and goat pancreas were predominantly associated with the acini, excretory ducts and blood vessels, but rarely detected in the pancreatic islets. The Gal-IR nerve cell bodies were observed as isolated bodies in the intra- and interlobular connective tissues and as a group within the intrapancreatic ganglia. At the vicinity of the nerve cell bodies, Gal-IR nerve fibers were observed. The present findings may suggest that: (1) galanin regulates pancreatic function as neurotransmitter/neuromodulator in ruminants; (2) galanin plays a more important role in large than in small ruminants; and (3) particularly in cattle, it exerts its most dramatic effect during perinatal development.
Baltazar
E T
ET
Department of Veterinary Anatomy, Obihiro University of Agriculture and Veterinary Medicine, Japan.
Kitamura
N
N
Sasaki
M
M
Cottrell
D F
DF
Boloron
H M
HM
Yamada
J
J
D000818:Animals; D002417:Cattle / Q000378:metabolism*; D005314:Embryonic and Fetal Development / Q000502:physiology; D005260:Female; D019004:Galanin / Q000378:metabolism*; D006041:Goats / Q000378:metabolism*; D007150:Immunohistochemistry / Q000662:veterinary; D007515:Islets of Langerhans / Q000033:anatomy & histology; D008297:Male; D009474:Neurons / Q000378:metabolism*; D010179:Pancreas / Q000294:innervation / Q000378:metabolism*; D011247:Pregnancy; D012756:Sheep / Q000378:metabolism*
D019004:Galanin