Large deletion of part of the HMGIC locus accompanying a t(3;12)(q27 approximately q28;q14 approximately q15) in a lipoma.
The high mobility group protein gene HMGIC has been found to be rearranged in a variety of human benign solid tumors. Often, these rearrangements lead to fusion genes of which that between HMGIC and LPP underlying t(3;12)(q27 approximately q28;q14 approximately q15) is by far the most common. Herein, we analysed a lipoma with a t(3;12)(q27 approximately q28;q14 approximately q15). RT-PCR revealed the presence of a HMGIC-LPP fusion transcript composed of exons 1-3 of HMGIC and exons 9-11 of LPP, but the absence of the reverse LPP-HMGIC fusion transcript. Fluorescence in situ hybridization experiments using different probes derived from the HMGIC gene and its 3' vicinity showed the absence of FISH signals on the derivative chromosome 3. Thus, in the present tumor the t(3;12)(q27 approximately q28;q14 approximately q15) was accompanied by a large genomic deletion. Roughly, it can be estimated that at least 170 kb of chromosome 12 material were deleted. To the best of our knowledge, this is the first report showing that the "simple" t(3;12)(q27 approximately q28;q14 approximately q15) results in a large deletion of DNA.
Lemke
I
I
Center of Human Genetics, University of Bremen, Leobenerstr. ZHG, D-28359, Bremen, Germany.
Rogalla
P
P
Bullerdiek
J
J
D002881:Chromosomes, Human, Pair 12 / Q000235:genetics*; D002893:Chromosomes, Human, Pair 3 / Q000235:genetics*; D003598:Cytoskeletal Proteins / Q000235:genetics; D025743:HMGA2 Protein; D006609:High Mobility Group Proteins / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D060588:LIM Domain Proteins; D008067:Lipoma / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017384:Sequence Deletion; D014178:Translocation, Genetic / Q000235:genetics*; D014407:Tumor Cells, Cultured
D003598:Cytoskeletal Proteins; D025743:HMGA2 Protein; D006609:High Mobility Group Proteins; D060588:LIM Domain Proteins; C103594:LPP protein, human