title: alpha1-acid glycoprotein possesses in vitro pro- and antiinflammatory activities.
abstract: We studied the effects of alpha1-acid glycoprotein on tumor necrosis factor-alpha (TNF-alpha) and interleukin-10 (IL-10) production and lymphocyte response to phytohemagglutinin in cultured peripheral blood mononuclear leukocytes from 6 healthy donors. We observed 2 opposite responses to alpha1-acid glycoprotein: first, stimulation of TNF-alpha and IL-10 production and inhibition of lymphocyte proliferation, and second, suppression of cytokine production and stimulation of lymphocyte proliferation. In cell cultures isolated from 4 of 6 donors, the TNF-alpha/IL-10 ratio remained unchanged after addition of native alpha1-acid glycoprotein, but some fractions isolated by chromatography on concanavalin A-Sepharose changed this parameter. These changes were most pronounced after treatment with fraction C enriched with molecules with incomplete (biantennary) carbohydrate chains. The mechanisms of alpha1-acid glycoprotein-induced effects on peripheral blood mononuclear leukocytes are discussed.
authors.affiliation: Methodical-and-Genetic Research Center, Russian Academy of Medical Sciences, Moscow. vikgor@arstel.ru
authors.forename: A L
authors.initials: AL
authors.lastname: Pukhal'skii
authors.forename: G V
authors.initials: GV
authors.lastname: Shmarina
authors.forename: A G
authors.initials: AG
authors.lastname: Lyutov
authors.forename: L I
authors.initials: LI
authors.lastname: Novikova
authors.forename: I G
authors.initials: IG
authors.lastname: Shemyakin
authors.forename: V A
authors.initials: VA
authors.lastname: Aleshkin
mesh_terms: D002455:Cell Division; D002478:Cells, Cultured; D003208:Concanavalin A / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007249:Inflammation / Q000378:metabolism*; D016753:Interleukin-10 / Q000096:biosynthesis; D007962:Leukocytes / Q000378:metabolism; D008954:Models, Biological; D009961:Orosomucoid / Q000378:metabolism*; D010835:Phytohemagglutinins / Q000378:metabolism; D012685:Sepharose / Q000737:chemistry; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000378:metabolism
chemical_list: D009961:Orosomucoid; D010835:Phytohemagglutinins; D014409:Tumor Necrosis Factor-alpha; D003208:Concanavalin A; D016753:Interleukin-10; D012685:Sepharose