title: Cell culture for three-dimensional modeling in rotating-wall vessels:  an application of simulated microgravity.
abstract: High-density, three-dimensional cell cultures are difficult to grow in vitro. The rotating-wall vessel (RWV) described here has cultured BHK-21 cells to a density of 1.1 X 10(7) cells/ml. Cells on microcarriers were observed to grow with enhanced bridging in this batch culture system. The RWV is a horizontally rotated tissue culture vessel with silicon membrane oxygenation. This design results in a low-turbulence, low-shear cell culture environment with abundant oxygenation. The RWV has the potential to culture a wide variety of normal and neoplastic cells.
authors.affiliation: KRUG Life Sciences, Houston, Texas 77058, USA.
authors.forename: R P
authors.initials: RP
authors.lastname: Schwarz
authors.forename: T J
authors.initials: TJ
authors.lastname: Goodwin
authors.forename: D A
authors.initials: DA
authors.lastname: Wolf
mesh_terms: D000818:Animals; D019149:Bioreactors; D002452:Cell Count; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods*; D002460:Cell Line; D006224:Cricetinae; D004867:Equipment Design; D007668:Kidney / Q000166:cytology; D008863:Microspheres; D012399:Rotation; D018474:Weightlessness Simulation