title: Acceleration of nucleic acid hybridization on DNA microarrays driven by pH tunable modifications.
abstract: A series of peptides containing histidine residues were designed as potential hybridization rate enhancers within a polymeric matrix of DNA microarrays. The polymeric matrix modified with these peptides showed strong attraction to DNA molecules under conditions of induction. DNA probes on the peptide-modified sites rapidly hybridized to their complementary targets with single base pair mismatch discrimination.
authors.affiliation: Motorola BioChip Systems, Tempe, Arizona, USA.
authors.forename: P
authors.initials: P
authors.lastname: Zhang
authors.forename: N
authors.initials: N
authors.lastname: Briones
authors.forename: C G
authors.initials: CG
authors.lastname: Liu
authors.forename: C K
authors.initials: CK
authors.lastname: Brush
authors.forename: T
authors.initials: T
authors.lastname: Powdrill
authors.forename: Y
authors.initials: Y
authors.lastname: Belosludtsev
authors.forename: M
authors.initials: M
authors.lastname: Hogan
mesh_terms: D004247:DNA / Q000235:genetics / Q000378:metabolism; D015342:DNA Probes; D015870:Gene Expression; D006639:Histidine / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D009693:Nucleic Acid Hybridization / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis; D010455:Peptides / Q000737:chemistry*; D013379:Substrate Specificity
chemical_list: D015342:DNA Probes; D010455:Peptides; D006639:Histidine; D004247:DNA