title: Characterization of the stability and folding of H2A.Z chromatin particles: implications for transcriptional activation.
abstract: H2A.Z and H2A.1 nucleosome core particles and oligonucleosome arrays were obtained using recombinant versions of these histones and a native histone H2B/H3/H4 complement reconstituted onto appropriate DNA templates. Analysis of the reconstituted nucleosome core particles using native polyacrylamide gel electrophoresis and DNase I footprinting showed that H2A.Z nucleosome core particles were almost structurally indistinguishable from its H2A.1 or native chicken erythrocyte counterparts. While this result is in good agreement with the recently published crystallographic structure of the H2A.Z nucleosome core particle (Suto, R. K., Clarkson, M J., Tremethick, D. J., and Luger, K. (2000) Nat. Struct. Biol. 7, 1121-1124), the ionic strength dependence of the sedimentation coefficient of these particles exhibits a substantial destabilization, which is most likely the result of the histone H2A.Z-H2B dimer binding less tightly to the nucleosome. Analytical ultracentrifuge analysis of the H2A.Z 208-12, a DNA template consisting of 12 tandem repeats of a 208-base pair sequence derived from the sea urchin Lytechinus variegatus 5 S rRNA gene, reconstituted oligonucleosome complexes in the absence of histone H1 shows that their NaCl-dependent folding ability is significantly reduced. These results support the notion that the histone H2A.Z variant may play a chromatin-destabilizing role, which may be important for transcriptional activation.
authors.affiliation: Department of Biochemistry and Microbiology, University of Victoria, Victoria, British Columbia V8W 3P6, Canada.
authors.forename: D W
authors.initials: DW
authors.lastname: Abbott
authors.forename: V S
authors.initials: VS
authors.lastname: Ivanova
authors.forename: X
authors.initials: X
authors.lastname: Wang
authors.forename: W M
authors.initials: WM
authors.lastname: Bonner
authors.forename: J
authors.initials: J
authors.lastname: Ausió
mesh_terms: D002843:Chromatin / Q000737:chemistry*; D006657:Histones / Q000737:chemistry*; D009707:Nucleosomes / Q000737:chemistry*; D017510:Protein Folding; D015533:Transcriptional Activation
chemical_list: D002843:Chromatin; D006657:Histones; D009707:Nucleosomes