title: Monocyte integrin expression and monocyte-platelet complex formation in humans with coronary restenosis.
abstract: 1. In the present study, we sought to determine whether patients with restenosis after coronary stenting possess increased monocyte reactivity, as manifested by a higher level of adhesion molecule expression and an enhanced propensity to form monocyte-platelet aggregates after activation in vitro. 2. Anti-coagulated peripheral venous blood from 24 patients, 10 with and 14 without angiographically verified restenosis, was obtained. Leucocyte antigen expression and the number of leucocyte-platelet complexes were measured by flow cytometry after activation in whole blood. 3. Surface integrin Mac-1 (CD11b/CD18) and VLA-4 (CD49d/ CD29) expression on monocytes and the relative number of monocyte-platelet complexes after in vitro activation were significantly elevated in patients with restenosis compared with patients without restenosis (fluorescence intensities of 1425 +/- 76 vs 1195 +/- 71, 87 +/- 7 vs 65 +/- 6 and 47 +/- 4 vs 29 +/- 3% for for Mac-1, VLA-4 and monocyte-platelet complexes, respectively; P < 0.05 for each parameter). 4. The results suggest that restenosis is associated with increased monocyte VLA-4 and Mac-1 integrin expression and monocyte-platelet complex formation, which can be revealed after activation in vitro.
authors.affiliation: Cardiology Research Center of Ministry of Health, Moscow, Russia.
authors.forename: T I
authors.initials: TI
authors.lastname: Arefieva
authors.forename: S I
authors.initials: SI
authors.lastname: Provatorov
authors.forename: A N
authors.initials: AN
authors.lastname: Samko
authors.forename: T L
authors.initials: TL
authors.lastname: Krasnikova
authors.forename: T J
authors.initials: TJ
authors.lastname: Resink
authors.forename: P
authors.initials: P
authors.lastname: Erne
authors.forename: V A
authors.initials: VA
authors.lastname: Tkachuk
authors.forename: E I
authors.initials: EI
authors.lastname: Chazov
mesh_terms: D001792:Blood Platelets / Q000378:metabolism* / Q000473:pathology; D023903:Coronary Restenosis / Q000378:metabolism* / Q000473:pathology*; D006801:Humans; D039041:Integrin alpha4beta1; D016023:Integrins / Q000096:biosynthesis*; D016014:Linear Models; D018950:Lipopolysaccharide Receptors / Q000096:biosynthesis; D016177:Macrophage-1 Antigen / Q000096:biosynthesis; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism* / Q000473:pathology; D010865:Pilot Projects; D016201:Receptors, Lymphocyte Homing / Q000096:biosynthesis
chemical_list: D039041:Integrin alpha4beta1; D016023:Integrins; D018950:Lipopolysaccharide Receptors; D016177:Macrophage-1 Antigen; D016201:Receptors, Lymphocyte Homing