Modelling a binuclear metal binding site in the photosynthetic reaction centre II.
Based on the experimental data and homologous sites in Protein Data Bank (PDB) a model for metal binding sites in D1/D2 heterodimer has been proposed. On searching for tetranuclear and binuclear Mn binding sites in the PDB, a suitable sequence homology in thermolysin and D1 could be observed. From the homology and site-directed mutagenesis data, a model for binuclear Mn-Ca or Mn-Mn has been built and it is extended to a tetranuclear Mn centre.
Rath
L S
LS
Post Graduate Department of Chemistry, Rajendra College, Bolangir 767 002, Orissa, India.
Raval
M K
MK
D000595:Amino Acid Sequence; D001665:Binding Sites; D002118:Calcium / Q000737:chemistry; D016208:Databases, Factual; D056890:Eukaryota / Q000737:chemistry*; D008024:Ligands; D008345:Manganese / Q000737:chemistry; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D045322:Photosynthetic Reaction Center Complex Proteins; D011485:Protein Binding; D017386:Sequence Homology, Amino Acid
D008024:Ligands; D045322:Photosynthetic Reaction Center Complex Proteins; D008345:Manganese; D002118:Calcium