DNA sequence recognition by NF kappa B p50 homodimer: strict and obscure recognition sites in the binding sequence.
5-Formyl- and 5-(formylmethyl)-2'-deoxyuridines are introduced into a kappa B site instead of thymidine(s) in order to understand target sequence specificity of NF kappa B. It was found that one thymidine in the kappa B site is particularly important for the sequence specific recognition by NF kappa B.
Kittaka
A
A
Faculty of Pharmaceutical Sciences, Teikyo University, Sagamiko, Kanagawa 199-0195, Japan.
Takayama
H
H
Kurihara
M
M
Horii
C
C
Tanaka
H
H
Miyasaka
T
T
Inoue
J
J
D001483:Base Sequence; D001665:Binding Sites; D019281:Dimerization; D008958:Models, Molecular; D016328:NF-kappa B / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D052002:NF-kappa B p50 Subunit; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000378:metabolism; D013379:Substrate Specificity; D013936:Thymidine / Q000378:metabolism
D016328:NF-kappa B; D052002:NF-kappa B p50 Subunit; D009841:Oligonucleotides; D013936:Thymidine