Evaluation of quadrupole time-of-flight tandem mass spectrometry and ion-trap multiple-stage mass spectrometry for the differentiation of C-glycosidic flavonoid isomers.
LC-MS-MS is becoming a very important tool for the on-line identification of natural products in crude plant extracts. For an efficient use of this technique in the dereplication of natural products, a careful study of the parameters used to generate informative MS-MS spectra is needed. In this paper, the collision-induced dissociation (CID) MS-MS spectra of ubiquitous C-glycosidic flavonoids have been systematically studied using hybrid quadrupole time-of-flight and ion-trap (IT) mass analysers under various CID energy conditions. Efficient differentiation of flavonoid C-glycoside isomers was possible, based on the comparison of CID-MS-MS spectra of particular C-glycoside unit fragments. Striking differences between 6-C and 8-C flavonoid glycosides were especially observed in the product ion spectra of their 0.2X+ fragments ([M+H-120]+). Some guidelines for the on-line characterisation of C-glycosidic flavonoids by LC-MS-MS or LC-multiple-stage MS are given.
Waridel
P
P
Institut de Pharmacognosie et Phytochimie, Université de Lausanne, Lausanne, Switzerland.
Wolfender
J L
JL
Ndjoko
K
K
Hobby
K R
KR
Major
H J
HJ
Hostettmann
K
K
D005419:Flavonoids / Q000737:chemistry*; D006027:Glycosides / Q000737:chemistry*; D007536:Isomerism; D013058:Mass Spectrometry / Q000379:methods*
D005419:Flavonoids; D006027:Glycosides