title: Stimulation of phagocytosis and free radical production in murine macrophages by 50 Hz electromagnetic fields.
abstract: Effects of 50 Hz electromagnetic fields on phagocytosis and free radical production were examined in mouse bone marrow-derived macrophages. Macrophages were in vitro exposed to electromagnetic fields using different magnetic field densities (0.5-1.5 mT). Short-time exposure (45 min) to electromagnetic fields resulted in significantly increased phagocytic uptake (36.3% +/- 15.1%) as quantified by measuring the internalization rate of latex beads. Stimulation with 1 nM 12-0-tetradecanoylphorbol-13-acetate (TPA) showed the same increased phagocytic activity as 1 mT electromagnetic fields. However, co-exposure to electromagnetic fields and TPA showed no further increase of bead uptake, and therefore we concluded that because of the absence of additive effects, the electromagnetic fields-induced stimulation of mouse bone marrow-derived macrophages does not involve the protein kinase C signal transduction pathway. Furthermore, a significant increased superoxide production after exposure to electromagnetic fields was detected.
authors.affiliation: University of Rostock, Institute of Cell Biology and Biosystems Technology, Division of Environmental Physiology, Germany. myrtill.simko@biologie.uni-rostock.de
authors.forename: M
authors.initials: M
authors.lastname: Simkó
authors.forename: S
authors.initials: S
authors.lastname: Droste
authors.forename: R
authors.initials: R
authors.lastname: Kriehuber
authors.forename: D G
authors.initials: DG
authors.lastname: Weiss
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D004574:Electromagnetic Fields / Q000009:adverse effects*; D005609:Free Radicals / Q000528:radiation effects*; D019254:Genes, bcl-2 / Q000235:genetics*; D008264:Macrophages / Q000378:metabolism* / Q000528:radiation effects*; D051379:Mice; D010587:Phagocytosis / Q000528:radiation effects*; D013234:Stem Cells / Q000187:drug effects*; D013481:Superoxides / Q000528:radiation effects*; D013755:Tetradecanoylphorbol Acetate / Q000009:adverse effects* / Q000737:chemistry*
chemical_list: D005609:Free Radicals; D013481:Superoxides; D013755:Tetradecanoylphorbol Acetate