Association of actin with alpha crystallins.
The alpha crystallins are cytosolic proteins that co-localize and co-purify with actin-containing microfilaments.  Affinity column chromatography employing both covalently-coupled actin or alpha crystallin was used to demonstrate specific and saturable binding of actin with alpha crystallin.  This conclusion was confirmed by direct visualization of alpha aggregates bound to actin polymerized in vitro.  The significance of this interaction in relation to the functional properties of these two polypeptides will be discussed.
Gopalakrishnan
S
S
Division of Biology, NSCORT, Kansas State University, Manhattan 66506, USA.
Boyle
D
D
Takemoto
L
L
D008841:Actin Cytoskeleton / Q000737:chemistry; D000199:Actins / Q000032:analysis / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D002462:Cell Membrane / Q000737:chemistry; D002846:Chromatography, Affinity / Q000379:methods; D003459:Crystallins / Q000032:analysis / Q000378:metabolism*; D011108:Polymers; D011485:Protein Binding; D011489:Protein Denaturation; D012685:Sepharose
D000199:Actins; D003459:Crystallins; D011108:Polymers; D012685:Sepharose