title: Myosin Va bound to phagosomes binds to F-actin and delays microtubule-dependent motility.
abstract: We established a light microscopy-based assay that reconstitutes the binding of phagosomes purified from mouse macrophages to preassembled F-actin in vitro. Both endogenous myosin Va from mouse macrophages and exogenous myosin Va from chicken brain stimulated the phagosome-F-actin interaction. Myosin Va association with phagosomes correlated with their ability to bind F-actin in an ATP-regulated manner and antibodies to myosin Va specifically blocked the ATP-sensitive phagosome binding to F-actin. The uptake and retrograde transport of phagosomes from the periphery to the center of cells in bone marrow macrophages was observed in both normal mice and mice homozygous for the dilute-lethal spontaneous mutation (myosin Va null). However, in dilute-lethal macrophages the accumulation of phagosomes in the perinuclear region occurred twofold faster than in normal macrophages. Motion analysis revealed saltatory phagosome movement with temporarily reversed direction in normal macrophages, whereas almost no reversals in direction were observed in dilute-lethal macrophages. These observations demonstrate that myosin Va mediates phagosome binding to F-actin, resulting in a delay in microtubule-dependent retrograde phagosome movement toward the cell center. We propose an "antagonistic/cooperative mechanism" to explain the saltatory phagosome movement toward the cell center in normal macrophages.
authors.affiliation: Institut für Zellbiologie und Biosystemtechnik, FB Biowissenschaften, Universität Rostock, D-18051 Rostock, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Al-Haddad
authors.forename: M A
authors.initials: MA
authors.lastname: Shonn
authors.forename: B
authors.initials: B
authors.lastname: Redlich
authors.forename: A
authors.initials: A
authors.lastname: Blocker
authors.forename: J K
authors.initials: JK
authors.lastname: Burkhardt
authors.forename: H
authors.initials: H
authors.lastname: Yu
authors.forename: J A
authors.initials: JA
authors.lastname: Hammer
authors.forename: D G
authors.initials: DG
authors.lastname: Weiss
authors.forename: W
authors.initials: W
authors.lastname: Steffen
authors.forename: G
authors.initials: G
authors.lastname: Griffiths
authors.forename: S A
authors.initials: SA
authors.lastname: Kuznetsov
mesh_terms: D000199:Actins / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001921:Brain; D048429:Cell Size; D002478:Cells, Cultured; D002645:Chickens; D003600:Cytosol / Q000378:metabolism; D017353:Gene Deletion; D008264:Macrophages / Q000166:cytology / Q000378:metabolism; D051379:Mice; D008817:Mice, Mutant Strains; D008840:Microfilament Proteins / Q000302:isolation & purification / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D008863:Microspheres; D008870:Microtubules / Q000378:metabolism*; D008970:Molecular Weight; D009038:Motion; D018995:Myosin Heavy Chains / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D024701:Myosin Type V / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D010588:Phagosomes / Q000737:chemistry / Q000378:metabolism*; D010641:Phenotype; D011485:Protein Binding
chemical_list: D000199:Actins; D008840:Microfilament Proteins; C513082:Myo5a protein, mouse; D000255:Adenosine Triphosphate; D024701:Myosin Type V; D018995:Myosin Heavy Chains