title: Hyaluronic acid increases motility/intracellular CA2+ concentration in human sperm in vitro.
abstract: This study investigated the mechanisms of the stimulatory effect of hyaluronic acid on motility in human sperm in vitro. A method, involving the measurement of forward progression through an agarose gel. was used to measure sperm motility quantitatively. Changes in intracellular Ca2+ concentrations in sperm were detected using the fluorescent dye Fluo-3. The effects of hyaluronic acid (6.5, 65, 650 ng/mL) and nifedipine (32 nM) on sperm motility were investigated. The effects of hyaluronic acid, nifedipine (32 nM), A23187 (32 microM), and a monoclonal antibody to human CD44 (1 microg/mL) on changes in intracellular CA2+ concentrations were investigated. Hyaluronic acid significantly (p < .008) stimulated sperm motility and this was partially inhibited by nifedipine. A23187 significantly (p < .005) increased intracellular CA2+ concentrations. Hyaluronic acid significantly (p < .04) increased intracellular Ca2+ concentrations and this was inhibited by nifedipine and a monoclonal antibody to human CD44. Hyaluronic acid stimulated human sperm motility by increasing intracellular Ca2+ concentration, partially via an influx of extracellular Ca2+.
authors.affiliation: Division of Biomedical Sciences, School of Health Sciences, University of Wolverhampton, UK.
authors.forename: R
authors.initials: R
authors.lastname: Bains
authors.forename: D M
authors.initials: DM
authors.lastname: Miles
authors.forename: R J
authors.initials: RJ
authors.lastname: Carson
authors.forename: J
authors.initials: J
authors.lastname: Adeghe
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D002118:Calcium / Q000378:metabolism*; D002121:Calcium Channel Blockers / Q000494:pharmacology; D006801:Humans; D018960:Hyaluronan Receptors / Q000276:immunology; D006820:Hyaluronic Acid / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D008297:Male; D009543:Nifedipine / Q000494:pharmacology; D013081:Sperm Motility / Q000187:drug effects*; D013094:Spermatozoa / Q000187:drug effects* / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D002121:Calcium Channel Blockers; D018960:Hyaluronan Receptors; D006820:Hyaluronic Acid; D009543:Nifedipine; D002118:Calcium