title: New approaches towards fluorescence labelling of messenger RNA transcripts.
abstract: Fluorescence labelling of nucleic acids is being used for a wide range of biological applications. The performance of these techniques is dependent on fluorochrome labels with a high sensitivity and high resistance to photobleaching. Indo-cyanine dyes such as Cy3, Cy5 or Cy7 have been found to fulfill these requirements. This study describes several different RNA labelling techniques allowing for a Cy5 based detection of mRNA transcripts.
authors.affiliation: Novartis Pharma AG, CH-4002 Basel, Switzerland.
authors.forename: A
authors.initials: A
authors.lastname: Garnier
authors.forename: D
authors.initials: D
authors.lastname: Hüsken
authors.forename: J
authors.initials: J
authors.lastname: Weiler
mesh_terms: D002232:Carbocyanines / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D012333:RNA, Messenger / Q000737:chemistry*
chemical_list: D002232:Carbocyanines; D005456:Fluorescent Dyes; D012333:RNA, Messenger; C085321:cyanine dye 5