Chemical synthesis of oligoribonucleotides containing 2-aminopurine:  substrates for the investigation of ribozyme function.
The chemical synthesis of a fully protected ribonucleoside phosphoramidite, containing 2-aminopurine as the base component, and its incorporation into short oligoribonucleotides as substrates for an engineered ribozyme from Tetrahymena is described.
Doudna
J A
JA
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA.
Szostak
J W
JW
Rich
A
A
Usman
N
N
D015075:2-Aminopurine / Q000031:analogs & derivatives / Q000737:chemistry*; D000818:Animals; D019020:Directed Molecular Evolution; D006151:Guanosine / Q000737:chemistry; D008967:Molecular Biology / Q000379:methods; D015394:Molecular Structure; D009843:Oligoribonucleotides / Q000138:chemical synthesis*; D016337:RNA, Catalytic / Q000737:chemistry* / Q000378:metabolism; D012263:Ribonucleosides / Q000737:chemistry*; D013379:Substrate Specificity; D013768:Tetrahymena / Q000201:enzymology*
D009843:Oligoribonucleotides; D016337:RNA, Catalytic; D012263:Ribonucleosides; D006151:Guanosine; D015075:2-Aminopurine