Four related proteins of the Trypanosoma brucei RNA editing complex.
RNA editing in kinetoplastid mitochondria occurs by a series of enzymatic steps that is catalyzed by a macromolecular complex. Four novel proteins and their corresponding genes were identified by mass spectrometric analysis of purified editing complexes from Trypanosoma brucei. These four proteins, TbMP81, TbMP63, TbMP42, and TbMP18, contain conserved sequences to various degrees. All four proteins have sequence similarity in the C terminus; TbMP18 has considerable sequence similarity to the C-terminal region of TbMP42, and TbMP81, TbMP63, and TbMP42 contain zinc finger motif(s). Monoclonal antibodies that are specific for TbMP63 and TbMP42 immunoprecipitate in vitro RNA editing activities. The proteins are present in the immunoprecipitates and sediment at 20S along with the in vitro editing, and RNA editing ligases TbMP52 and TbMP48. Recombinant TbMP63 and TbMP52 coimmunoprecipitate. These results indicate that these four proteins are components of the RNA editing complex and that TbMP63 and TbMP52 can interact.
Panigrahi
A K
AK
Seattle Biomedical Research Institute, Seattle, Washington 98109, USA.
Schnaufer
A
A
Carmean
N
N
Igo
R P
RP
Gygi
S P
SP
Ernst
N L
NL
Palazzo
S S
SS
Weston
D S
DS
Aebersold
R
R
Salavati
R
R
Stuart
K D
KD
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D002352:Carrier Proteins / Q000737:chemistry; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D008025:Ligases / Q000378:metabolism; D013058:Mass Spectrometry; D008957:Models, Genetic; D008969:Molecular Sequence Data; D011233:Precipitin Tests; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D015800:Protozoan Proteins; D017393:RNA Editing; D012333:RNA, Messenger / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D012261:Ribonucleoproteins / Q000737:chemistry; D017386:Sequence Homology, Amino Acid; D014346:Trypanosoma brucei brucei / Q000235:genetics* / Q000378:metabolism*; D016335:Zinc Fingers
D002352:Carrier Proteins; D015800:Protozoan Proteins; D012333:RNA, Messenger; D011994:Recombinant Proteins; D012261:Ribonucleoproteins; C438000:TbMP18 protein, Trypanosoma brucei; C437999:TbMP42 protein, Trypanosoma brucei; C437998:TbMP63 protein, Trypanosoma brucei; C437997:TbMP81 protein, Trypanosoma brucei; D008025:Ligases