Syntheses of DNA duplexes containing a C-C interstrand cross-link.
Short DNA duplexes that contain a N4C-ethyl-N4C interstrand cross-link were prepared on controlled pore glass supports using a DNA synthesizer. The C-C cross-link was introduced via a convertible nucleoside on the support or by using a protected C-C cross-link phosphoramidite. An orthogonal protection scheme allowed selective chain growth in either a 3'-->5' or 5'-->3' direction. The cross-linked duplexes were purified by HPLC and characterized by MALDI-TOF mass spectrometry and/or by enzymatic digestion.
Noronha
A M
AM
Johns Hopkins University, Department of Biochemistry and Molecular Biology, School of Hygiene and Public Health, 615 North Wolfe Street, Baltimore, Maryland, USA.
Noll
D M
DM
Miller
P S
PS
D003432:Cross-Linking Reagents / Q000737:chemistry*; D004247:DNA / Q000138:chemical synthesis*; D009705:Nucleosides / Q000737:chemistry; D009943:Organophosphorus Compounds / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014230:Triazoles / Q000737:chemistry
D003432:Cross-Linking Reagents; D009705:Nucleosides; D009943:Organophosphorus Compounds; D014230:Triazoles; C434331:phosphoramidite; D004247:DNA