Cellular uptake and intracellular quantification of fluorescent labeled T20 Me-SATE prooligonucleotides.
Fluorescein labeled Me-SATE T20 models were synthesized. The high uptake of these prooligos in HeLa cells was confirmed by fluorescence microscopy, flow cytometry and by spectrofluorometry.
Morvan
F
F
Laboratoire de Chimie Organique Biomoléculaire de Synthèse, UMR 5625 CNRS-UM II, Université Montpellier II, Place E. Bataillon, 34095 Montpellier, France.
Bologna
J C
JC
Vivès
E
E
Imbach
J L
JL
D005452:Fluoresceins / Q000032:analysis / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D008856:Microscopy, Fluorescence; D009841:Oligonucleotides / Q000032:analysis / Q000493:pharmacokinetics*; D011742:Pyrimidine Nucleotides / Q000032:analysis / Q000493:pharmacokinetics; D013936:Thymidine / Q000031:analogs & derivatives* / Q000032:analysis / Q000493:pharmacokinetics
D005452:Fluoresceins; D009841:Oligonucleotides; D011742:Pyrimidine Nucleotides; D013936:Thymidine