title: Letinula edodes (Berk.) Pegler (Shiitake) modulates genotoxic and mutagenic effects induced by alkylating agents in vivo.
abstract: We evaluated the antimutagenic effect of Letinula edodes (Berk.) Pegler (Shiitake) on the frequency of micronuclei in mice treated with N-ethyl-N-nitrosourea (ENU) or cyclophosphamide (CP). Mice were orally (gavage) pretreated for 15 consecutive days with solutions of Shiitake (0.6 ml per day, gavage) prepared at three different temperatures: 4, 21 (RT), and 60 degrees C. Then, the animals were intraperitoneally injected on day 15 with CP (25 or 50mg/kg) or ENU (50 mg/kg) and killed 24 or 48 h after treatment for evaluation of micronucleated polychromatic erythrocytes (MNPCEs) in bone marrow and micronucleated reticulocytes (MNRETs). A mixture of L. edodes lineages (LE 95/016, 96/14, 96/17, 96/22, 96/23, 97/27, and 97/28) significantly decreased the frequencies of MNPCEs and MNRETs induced by CP (25 and 50mg/kg). When a single lineage from the mixture (LE 96/17) was tested we also found a significant reduction in the frequencies of MNPCEs and MNRETs induced by both CP or ENU (50mg/kg). The comet assay was also performed 3h after ENU treatment using mice pretreated with the single lineage (LE 96/17) of L. edodes. The results showed a high degree of variability with some indications of an antigenotoxic effect. Taken together, our data show that solutions from Shiitake inhibit in vivo mutagenicity of CP and ENU.
authors.affiliation: Departamento de Patologia, Faculdade de Medicina, UNESP, 18618-000, SP, Botucatu, Brazil.
authors.forename: P L
authors.initials: PL
authors.lastname: de Lima
authors.forename: R D
authors.initials: RD
authors.lastname: Delmanto
authors.forename: M M
authors.initials: MM
authors.lastname: Sugui
authors.forename: A F
authors.initials: AF
authors.lastname: da Eira
authors.forename: D M
authors.initials: DM
authors.lastname: Salvadori
authors.forename: G
authors.initials: G
authors.lastname: Speit
authors.forename: L R
authors.initials: LR
authors.lastname: Ribeiro
mesh_terms: D000818:Animals; D016587:Antimutagenic Agents / Q000494:pharmacology*; D001854:Bone Marrow Cells / Q000187:drug effects / Q000473:pathology; D002452:Cell Count; D020552:Comet Assay; D003520:Cyclophosphamide / Q000633:toxicity; D004249:DNA Damage / Q000187:drug effects*; D004912:Erythrocytes / Q000187:drug effects; D005038:Ethylnitrosourea / Q000633:toxicity; D008297:Male; D051379:Mice; D015162:Micronucleus Tests; D009153:Mutagens / Q000633:toxicity; D010936:Plant Extracts / Q000494:pharmacology*; D012156:Reticulocytes / Q000187:drug effects / Q000473:pathology; D020942:Shiitake Mushrooms / Q000737:chemistry*; D013696:Temperature
chemical_list: D016587:Antimutagenic Agents; D009153:Mutagens; D010936:Plant Extracts; D003520:Cyclophosphamide; D005038:Ethylnitrosourea