Clinorotation affects morphology and ethylene production in soybean seedlings.
The microgravity environment of spaceflight influences growth, morphology and metabolism in etiolated germinating soybean. To determine if clinorotation will similarly impact these processes, we conducted ground-based studies in conjunction with two space experiment opportunities. Soybean (Glycine max [L.] Merr.) seeds were planted within BRIC (Biological Research In Canister) canisters and grown for seven days at 20 degrees C under clinorotation (1 rpm) conditions or in a stationary upright mode. Gas samples were taken daily and plants were harvested after seven days for measurement of growth and morphology. Compared to the stationary upright controls, plants exposed to clinorotation exhibited increased root length (125% greater) and fresh weight (42% greater), whereas shoot length and fresh weight decreased by 33% and 16% respectively. Plants grown under clinorotation produced twice as much ethylene as the stationary controls. Seedlings treated with triiodo benzoic acid (TIBA), an auxin transport inhibitor, under clinorotation produced 50% less ethylene than the untreated control subjected to the same gravity treatment, whereas a treatment with 2,4-D increased ethylene by five-fold in the clinorotated plants. These data suggest that slow clinorotation influences biomass partitioning and ethylene production in etiolated soybean plants.
Hilaire
E
E
Dynamac Corporation, Kennedy Space Center, FL 32899, USA.
Peterson
B V
BV
Guikema
J A
JA
Brown
C S
CS
D015084:2,4-Dichlorophenoxyacetic Acid / Q000494:pharmacology; D002245:Carbon Dioxide / Q000378:metabolism; D005030:Ethylenes / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D006112:Gravitation; D006540:Herbicides / Q000494:pharmacology; D007210:Indoleacetic Acids / Q000037:antagonists & inhibitors / Q000378:metabolism; D010937:Plant Growth Regulators / Q000096:biosynthesis* / Q000494:pharmacology; D018517:Plant Roots / Q000033:anatomy & histology / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D018520:Plant Shoots / Q000033:anatomy & histology / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D012399:Rotation / Q000009:adverse effects*; D013025:Glycine max / Q000033:anatomy & histology / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D014283:Triiodobenzoic Acids / Q000494:pharmacology; D018474:Weightlessness Simulation
D005030:Ethylenes; D006540:Herbicides; D007210:Indoleacetic Acids; D010937:Plant Growth Regulators; D014283:Triiodobenzoic Acids; D002245:Carbon Dioxide; D015084:2,4-Dichlorophenoxyacetic Acid; C036216:ethylene