Androgen receptor isoforms in human prostatic cancer tissue and LNCaP cell line.
OBJECTIVE
To investigate the androgen receptor (AR) isoform expressions in human prostatic cancer tissue and LNCaP cell line.


METHODS
With high resolution isoelectric focusing (IEF) method we demonstrated the different expressions of AR isoforms in human prostatic cancer tissues and LNCaP cell line.


RESULTS
Data were obtained from three prostatic cancer specimens and the LNCaP cell line. Three types of AR isoforms were detected with pI values at 6.5, 6.0, and 5.3. For the 3 prostatic cancer specimens, 1 sample showed all the three types of AR isoforms, the second specimen expressed at 6.5 and 6.0, and the third failed to show any type of isoforms. The LNCaP cell line expressed all the three AR isoforms. Binding of 3H-dihydrotestosterone (3H-DHT) to these three isoforms was inhibited by the addition of 100-fold excess of DHT or testosterone, while not by progesterone, oestradiol and diethylstilboestrol.


CONCLUSIONS
The expression of AR isoforms is different in different prostate cancer tissues, which may be related to the difference in the effect of anti-androgen therapy in different patients.
Xia
S J
SJ
University Department of Urology, Shandong Provincial Hospital Jinan, China. xiashujie@yahoo.com
Tang
X D
XD
Ma
Q Z
QZ
D013196:Dihydrotestosterone / Q000378:metabolism / Q000494:pharmacology; D006801:Humans; D007525:Isoelectric Focusing; D007536:Isomerism; D008297:Male; D011471:Prostatic Neoplasms / Q000737:chemistry*; D011869:Radioligand Assay; D011944:Receptors, Androgen / Q000032:analysis* / Q000737:chemistry; D014316:Tritium; D014407:Tumor Cells, Cultured / Q000737:chemistry
D011944:Receptors, Androgen; D013196:Dihydrotestosterone; D014316:Tritium