title: Detection of Slackia exigua, Mogibacterium timidum and Eubacterium saphenum from pulpal and periradicular samples using the Polymerase Chain Reaction (PCR) method.
abstract: OBJECTIVE
  The purpose of this study was to detect Slackia exigua from root canal samples using a sensitive PCR amplification method. Mogibacterium timidum and Eubacterium saphenum were also included because of their culture-difficult properties.
  
  
  METHODS
  The species-specific PCR primers were prepared according to 16S rDNA sequence analysis data, and confirmed to be effective for PCR amplification as species-specific, respectively. A total of 36 clinical samples were obtained during the first visit of root canal treatment.
  
  
  RESULTS
  The sensitivity of detection was a minimum of 10 organisms for S. exigua and five organisms for M. timidum and E. saphenum, respectively. In seven cases of pulpitis, Sexigua was detected in two cases (29%), and M. timidum in two cases (29%), but E. saphenum was not detected. In 17 cases of root canal treatment, S. exigua was detected in seven cases (41%), M. timidum in 12 cases (71%) and E. saphenum in four cases (24%). In 12 cases of root canal retreatment, S. exigua was detected in three cases (25%), M. timidum in three cases (25%) and E. saphenum in two cases (17%).
  
  
  CONCLUSIONS
  S. exigua, M. timidum and E. saphenum were present in root canal systems, and may be associated with pulpal and periradicular pathosis.
authors.affiliation: Department of Oral Microbiology, Niigata University School of Dentistry, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Hashimura
authors.forename: M
authors.initials: M
authors.lastname: Sato
authors.forename: E
authors.initials: E
authors.lastname: Hoshino
mesh_terms: D000193:Actinomycetales Infections / Q000382:microbiology*; D000293:Adolescent; D000328:Adult; D000368:Aged; D015373:Bacterial Typing Techniques; D016009:Chi-Square Distribution; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D003786:Dental Pulp Cavity / Q000382:microbiology; D003788:Dental Pulp Diseases / Q000382:microbiology*; D005051:Eubacterium / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010485:Periapical Periodontitis / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal