title: Molecular cloning and functional expression of bovine brain GABA transaminase.
abstract: We isolated a cDNA that encodes the bovine brain gamma-aminobutyrate transaminase (GABA-T; EC 2.6.1.19) from the lambda gt 11 cDNA library, which showed a high degree of sequence similarity to the corresponding enzymes from various sources. Northern blot analysis revealed two differentially expressed GABA-T transcripts of approximately 2.0 and 6.0 kb in the bovine tissues. Southern blot analysis indicates that the two GABA-T transcripts are encoded in a greater-than 10-kb, single-copy gene. Bovine GABA-T cDNA was expressed in E. coli using the pGEX bacterial- expression vector system. The overexpressed GABA-T was enzymatically active after purification, and it had very similar kinetic parameters when compared with those of other mammalian GABA-Ts.
authors.affiliation: Department of Genetic Engineering, Hallym University, Chunchon, Korea.
authors.forename: S G
authors.initials: SG
authors.lastname: Jeon
authors.forename: J H
authors.initials: JH
authors.lastname: Bahn
authors.forename: J S
authors.initials: JS
authors.lastname: Jang
authors.forename: S H
authors.initials: SH
authors.lastname: Jang
authors.forename: B R
authors.initials: BR
authors.lastname: Lee
authors.forename: K S
authors.initials: KS
authors.lastname: Lee
authors.forename: J
authors.initials: J
authors.lastname: Park
authors.forename: T C
authors.initials: TC
authors.lastname: Kang
authors.forename: M H
authors.initials: MH
authors.lastname: Won
authors.forename: H B
authors.initials: HB
authors.lastname: Kim
authors.forename: O S
authors.initials: OS
authors.lastname: Kwo
authors.forename: S W
authors.initials: SW
authors.lastname: Cho
authors.forename: S Y
authors.initials: SY
authors.lastname: Choi
mesh_terms: D000612:4-Aminobutyrate Transaminase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D001921:Brain / Q000201:enzymology* / Q000502:physiology; D002417:Cattle; D003001:Cloning, Molecular; D006801:Humans; D008969:Molecular Sequence Data; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism; D016415:Sequence Alignment; D014018:Tissue Distribution
chemical_list: D011993:Recombinant Fusion Proteins; D000612:4-Aminobutyrate Transaminase