Purification and characterization of a casein kinase 2-type protein kinase from pea nuclei.
Almost all the polyamine-stimulated protein kinase activity associated with the chromatin fraction of nuclei purified from etiolated pea (Pisum sativum L.) plumules is present in a single enzyme that can be extracted from chromatin by 0.35 molar NaCl.  This protein kinase can be further purified over 2000-fold by salt fractionation and anion-exchange and casein-agarose column chromatography, after which it is more than 90% pure.  The purified kinase has a specific activity of about 650 nanomoles per minute per milligram protein in the absence of polyamines, with either ATP or GTP as phosphoryl donor.  Spermidine can stimulate its activity fourfold, with half-maximal activation at about 2 millimolar.  Spermine and putrescine also stimulate activity, although somewhat less effectively.  This kinase has a tetrameric alpha 2 beta 2 structure with a native molecular weight of 130,000, and subunit molecular weights of 36,000 for the catalytic subunit (alpha) and 29,000 for the regulatory subunit (beta).  In western blot analyses, only the alpha subunit reacts strongly with polyclonal antibodies to a Drosophila casein kinase II.  The pea kinase can use casein and phosvitin as artificial substrates, phosphorylating both the serine and threonine residues of casein.  It has a pH optimum near 8.0, a Vmax of 1.5 micromoles per minute per milligram protein, and a Km for ATP of approximately 75 micromolar.  Its activity can be almost completely inhibited by heparin at 5 micrograms per milliliter, but is relatively insensitive to concentrations of staurosporine, K252a, and chlorpromazine that strongly antagonize Ca(2+) -regulated protein kinases.  These results are discussed in relation to recent findings that casein kinase 2-type kinases may phosphorylate trans-acting factors that bind to light-regulated promoters in plants.
Li
H
H
Department of Botany, The University of Texas at Austin 78713.
Roux
S J
SJ
D047390:Casein Kinase II; D002364:Caseins / Q000737:chemistry; D002467:Cell Nucleus / Q000201:enzymology*; D018744:DNA, Plant; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000187:drug effects / Q000302:isolation & purification; D018532:Pisum sativum / Q000201:enzymology* / Q000235:genetics; D010766:Phosphorylation; D010774:Phosvitin / Q000737:chemistry; D010940:Plant Proteins / Q000737:chemistry / Q000187:drug effects / Q000302:isolation & purification; D011073:Polyamines / Q000494:pharmacology; D011494:Protein Kinases / Q000737:chemistry / Q000187:drug effects / Q000302:isolation & purification*; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry / Q000187:drug effects / Q000302:isolation & purification*; D013379:Substrate Specificity
D002364:Caseins; D018744:DNA, Plant; D004268:DNA-Binding Proteins; D010940:Plant Proteins; D011073:Polyamines; D010774:Phosvitin; D011494:Protein Kinases; D047390:Casein Kinase II; D017346:Protein Serine-Threonine Kinases