[Effect of peptide immunomodulator gangleen on the activity of Yersinia pseudotuberculosis thermostable toxin in oxidant and antioxidant systems of human leukocytes].
The ability to correct activity of Y. pseudotuberculosis thermoresistant toxin on antioxidative enzymes and on active oxygen forms in neurophiles and mononuclears was investigated. Toxin at concentration 0.5 and 2.5 mcg/ml did not change O2- production and activity of superoxidedismutase (SOD) and glutathione peroxidase (GP), but significantly enhanced catalase and glutathiont reductase (GR) activity. Gangleen at concentration 0.0002-0.2 mcg/ml, when added to incubation medium with toxin, stimulated production of active oxygen forms and activity of SOD, catalase, GP in both types of leucocytes, but decreased activity of GR in mononuclears. The results of investigation proves the ability to use gangleen in correction of immune system disorders caused by Y. pseudotuberculosis thermoresistant toxin.
Logvinenko
A A
AA
Research Institute of Epidemiology and Microbiology, Siberian Branch of the Russian Academy of Medical Sciences, Pacific Ocean Institute, Pacific Ocean Research Institute of Fish Industry and Oceanography, Vladivostok.
Dolmatova
L S
LS
Timchenko
N F
NF
Epshteĭn
L M
LM
D000276:Adjuvants, Immunologic / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D001427:Bacterial Toxins / Q000737:chemistry / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D049832:Decapodiformes; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D066298:In Vitro Techniques; D007962:Leukocytes / Q000187:drug effects* / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000378:metabolism; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism; D010084:Oxidation-Reduction; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D013696:Temperature; D015011:Yersinia pseudotuberculosis / Q000737:chemistry*
D000276:Adjuvants, Immunologic; D001427:Bacterial Toxins; D010455:Peptides; D017382:Reactive Oxygen Species; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase