Seasonal variation in plasma lipids, lipoproteins, apolipoprotein A-I and vitellogenin in the freshwater turtle, Chrysemys picta.
An analysis of plasma lipids and lipoprotein fractions was performed over the course of the annual ovarian cycle of the female turtle, Chrysemys picta. Determinations of total plasma triglycerides, cholesterol, vitellogenin and apolipoprotein A-I (apoA-I) were made. The lipid and protein composition of the lipoprotein fractions [very low density lipoprotein (VLDL), low density lipoprotein (LDL), high density lipoprotein (HDL) and very high density lipoprotein (VHDL)] were also observed over the same period. Plasma triglyceride and vitellogenin levels were significantly increased in the spring preovulatory period and fall recrudescent phase. Total plasma cholesterol levels were significantly elevated only at the onset of the fall recrudescent phase and apoA-I levels were highest during the postoviposition/ovarian arrest phase. The triglyceride content of VLDL was highest in preovulatory animals and there were apparent seasonal changes in the expression of apoA-I and apoE of HDL/VHDL. We conclude that the coordinate regulation of lipids and protein contributes to seasonal ovarian growth and clearance of lipids from plasma, both of which are most likely under hormonal control.
Duggan
A
A
Department of Biology, Boston University, 5 Cummington Street, Boston, MA 02215, USA. ipc@bio.bu.edu
Paolucci
M
M
Tercyak
A
A
Gigliotti
M
M
Small
D
D
Callard
I
I
D000818:Animals; D016632:Apolipoprotein A-I / Q000097:blood*; D001798:Blood Proteins / Q000032:analysis; D002784:Cholesterol / Q000097:blood; D004586:Electrophoresis; D004591:Electrophoresis, Polyacrylamide Gel; D005227:Fatty Acids / Q000097:blood; D005260:Female; D008055:Lipids / Q000097:blood*; D008074:Lipoproteins / Q000097:blood* / Q000737:chemistry / Q000648:ultrastructure; D008854:Microscopy, Electron; D012098:Reproduction / Q000502:physiology; D012621:Seasons; D014280:Triglycerides / Q000097:blood; D014426:Turtles / Q000097:blood*; D014819:Vitellogenins / Q000097:blood*
D016632:Apolipoprotein A-I; D001798:Blood Proteins; D005227:Fatty Acids; D008055:Lipids; D008074:Lipoproteins; D014280:Triglycerides; D014819:Vitellogenins; D002784:Cholesterol