title: Contrast agent distribution in microvascular damage of infarcted pig myocardium.
abstract: OBJECTIVE
  We determined whether reperfusion damage was sufficient to allow extravasation of a large molecular weight contrast agent into infarcted pig myocardium.
  
  
  METHODS
  Five pig hearts were subjected to in situ occlusion of the left anterior descending coronary artery (2 h) followed by reperfusion (1 h). The hearts were excised and perfused in the Langendorff mode for ex vivo MR imaging. Polylysine-Gd-DTPA (50,000 Da) and Gd-DTPA (500-700 Da) were injected into the aorta (alternately) and followed by measurements of T1 relaxation and mean transit time (MTT).
  
  
  RESULTS
  In the normal myocardium, MTT of Gd-DTPA (56.8+/-23.2 s) was significantly (p=0.02) longer than that of polylysine-Gd-DTPA (29.0+/-7 s). However, both normal and infarcted myocardium showed similar MTT (29.0+/-7.0 vs. 28.0+/-5.0 s, p>0.05) when using polylysine-Gd-DTPA.
  
  
  CONCLUSIONS
  The results indicate that the permeability of capillaries to polylysine-Gd-DTPA was not significantly higher in infarcted regions of the myocardium compared to normal tissue. However, infarcted myocardium displayed an increased permeability to the small molecular weight Gd-DTPA. We conclude that microvascular damage may not be sufficient to allow the extravasation of polylysine-Gd-DTPA in infarcted myocardium.
authors.affiliation: Institute for Biodiagnostics, National Research Council Canada, Winnipeg, MB, Canada.
authors.forename: J
authors.initials: J
authors.lastname: Mark
authors.forename: G
authors.initials: G
authors.lastname: Dai
authors.forename: B
authors.initials: B
authors.lastname: Xiang
authors.forename: J
authors.initials: J
authors.lastname: Sun
authors.forename: W G
authors.initials: WG
authors.lastname: Lindsay
authors.forename: R
authors.initials: R
authors.lastname: Deslauriers
authors.forename: G
authors.initials: G
authors.lastname: Tian
mesh_terms: D000818:Animals; D003287:Contrast Media / Q000378:metabolism*; D005119:Extravasation of Diagnostic and Therapeutic Materials / Q000378:metabolism; D019786:Gadolinium DTPA / Q000378:metabolism; D008279:Magnetic Resonance Imaging; D009203:Myocardial Infarction / Q000378:metabolism*; D011107:Polylysine / Q000031:analogs & derivatives / Q000378:metabolism; D013552:Swine; D014018:Tissue Distribution
chemical_list: D003287:Contrast Media; C068674:polylysine-(Gd-DTPA); D011107:Polylysine; D019786:Gadolinium DTPA