title: Clinostation influence on regeneration of cell wall in Solanum tuberosum L. protoplasts.
abstract: Regeneration of cell walls in protoplasts was investigated using light- and electronmicroscopic methods.  The protoplasts were isolated from mesophyll of Solanum tuberosum leaves and were cultivated on the horizontal low rotating clinostat (2 rpm) and in control for 10 days.  Using a fluorescent method (with Calcofluor white) it was demonstrated that changes in vector gravity results in a regeneration inhibition of cell wall.  With electron-microscopical and electro-cytochemical methods (staining with alcianum blue) dynamics of the regeneration of cell walls in protoplasts was studied; carbohydrate matrix of cell walls is deposited at the earliest stages of this process.  The influence of microgravity on the cell wall regeneration is discussed in higher plants.
authors.affiliation: Institute of Botany, Academy of Sciences of Ukraine, Kiev.
authors.forename: E M
authors.initials: EM
authors.lastname: Nedukha
authors.forename: V A
authors.initials: VA
authors.lastname: Sidorov
authors.forename: V M
authors.initials: VM
authors.lastname: Samoylov
mesh_terms: D002473:Cell Wall / Q000502:physiology* / Q000648:ultrastructure; D002736:Chloroplasts / Q000502:physiology / Q000648:ultrastructure; D006112:Gravitation; D008854:Microscopy, Electron; D015388:Organelles / Q000648:ultrastructure; D011523:Protoplasts / Q000502:physiology* / Q000648:ultrastructure; D012038:Regeneration; D012399:Rotation; D011198:Solanum tuberosum / Q000166:cytology* / Q000502:physiology / Q000648:ultrastructure; D018474:Weightlessness Simulation