Evaluation of disinfectant efficacy against hepatitis C virus using a RT-PCR-based method.
The methods traditionally used to evaluate the antiviral activity of antiseptics and disinfectants are based on cell cultures. However, such methods are not applicable to non-cultivable viruses such as hepatitis C (HCV). Therefore, in this case, virucidal activity is normally tested using surrogate viruses able to grow in cell culture. This paper describes a RT-PCR method for testing antiseptic/disinfectant activity against HCV, as a model for non-cultivable viruses. A chlorine-based agent used for skin and tissues, and a 2% glutaraldehyde solution used for endoscope disinfection, were the test materials. The results are discussed in the light of the use of these agents. The method is simple, fast and inexpensive, and could be used for tests on other viruses with minor modification.
Charrel
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RN
Unité des Virus Emergents (EA 3292), Laboratoire de Virologie Moléculaire, Tropicale et Transfusionnelle, Faculté de Médecine, 27, boulevard Jean Moulin, Marseille 13005, France. rnc-virophdm@mail.gulliver.fr
de Chesse
R
R
Decaudin
A
A
De Micco
P
P
de Lamballerie
X
X
D004202:Disinfectants / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004341:Drug Evaluation; D005976:Glutaral / Q000494:pharmacology*; D016174:Hepacivirus / Q000187:drug effects* / Q000235:genetics; D006801:Humans; D012367:RNA, Viral / Q000187:drug effects; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012973:Sodium Hypochlorite / Q000494:pharmacology*
D004202:Disinfectants; D012367:RNA, Viral; D012973:Sodium Hypochlorite; D005976:Glutaral