title: Cd1d is expressed on dermal dendritic cells and monocyte-derived dendritic cells.
abstract: CD1 proteins are a family of cell surface molecules that present lipid antigens to T cells. We investigated skin dendritic cells and monocyte-derived dendritic cells for expression of CD1 molecules using a panel of 10 different monoclonal antibodies focusing on the recently described CD1d molecule. By immunohistochemical analysis, CD1d expression in normal human skin was restricted to dendritic appearing cells in the papillary dermis mainly located in a perivascular localization. Langerhans cells did not show detectable CD1d expression in situ. Epidermal/dermal cell suspensions analyzed by flow cytometry demonstrated distinct subpopulations of HLA-DR positive dermal dendritic cells expressing CD1a, CD1b, and CD1c. CD1d was expressed on HLA-DRbright dermal antigen-presenting cells in dermal suspensions (16% +/- 3.6%), as well as on highly enriched dermal dendritic cells migrating out of skin explants (60.5% +/- 8.0%). Migrated mature dermal dendritic cells coexpressed CD83 and CD1d. Western blot analysis on microdissected skin sections revealed the presence of a 50-55 kDa CD1d molecule in dermis, suggesting that CD1d is highly glycosylated in skin. Both immature and mature monocyte-derived dendritic cells cultured in autologous plasma expressed CD1d molecules. In contrast, culture in fetal bovine serum downregulated CD1d expression. In conclusion, antigen-presenting cells in skin express different sets of CD1 molecules including CD1d and might play a role in lipid antigen presentation in various skin diseases. Differential expression of CD1 molecules depending on culture conditions might have an impact on clinical applications of dendritic cells for immunotherapy.
authors.affiliation: Department of Dermatology, University of Zürich Medical School, Zürich, Switzerland.
authors.forename: G
authors.initials: G
authors.lastname: Gerlini
authors.forename: H P
authors.initials: HP
authors.lastname: Hefti
authors.forename: M
authors.initials: M
authors.lastname: Kleinhans
authors.forename: B J
authors.initials: BJ
authors.lastname: Nickoloff
authors.forename: G
authors.initials: G
authors.lastname: Burg
authors.forename: F O
authors.initials: FO
authors.lastname: Nestle
mesh_terms: D000818:Animals; D018949:Antigens, CD1 / Q000276:immunology*; D002417:Cattle; D002454:Cell Differentiation; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology*; D006801:Humans; D007150:Immunohistochemistry; D009000:Monocytes / Q000166:cytology / Q000276:immunology; D012867:Skin / Q000166:cytology / Q000276:immunology
chemical_list: D018949:Antigens, CD1