Stimulation of phagocytosis and free radical production in murine macrophages by 50 Hz electromagnetic fields.
Effects of 50 Hz electromagnetic fields on phagocytosis and free radical production were examined in mouse bone marrow-derived macrophages. Macrophages were in vitro exposed to electromagnetic fields using different magnetic field densities (0.5-1.5 mT). Short-time exposure (45 min) to electromagnetic fields resulted in significantly increased phagocytic uptake (36.3% +/- 15.1%) as quantified by measuring the internalization rate of latex beads. Stimulation with 1 nM 12-0-tetradecanoylphorbol-13-acetate (TPA) showed the same increased phagocytic activity as 1 mT electromagnetic fields. However, co-exposure to electromagnetic fields and TPA showed no further increase of bead uptake, and therefore we concluded that because of the absence of additive effects, the electromagnetic fields-induced stimulation of mouse bone marrow-derived macrophages does not involve the protein kinase C signal transduction pathway. Furthermore, a significant increased superoxide production after exposure to electromagnetic fields was detected.
Simkó
M
M
University of Rostock, Institute of Cell Biology and Biosystems Technology, Division of Environmental Physiology, Germany. myrtill.simko@biologie.uni-rostock.de
Droste
S
S
Kriehuber
R
R
Weiss
D G
DG
D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D004574:Electromagnetic Fields / Q000009:adverse effects*; D005609:Free Radicals / Q000528:radiation effects*; D019254:Genes, bcl-2 / Q000235:genetics*; D008264:Macrophages / Q000378:metabolism* / Q000528:radiation effects*; D051379:Mice; D010587:Phagocytosis / Q000528:radiation effects*; D013234:Stem Cells / Q000187:drug effects*; D013481:Superoxides / Q000528:radiation effects*; D013755:Tetradecanoylphorbol Acetate / Q000009:adverse effects* / Q000737:chemistry*
D005609:Free Radicals; D013481:Superoxides; D013755:Tetradecanoylphorbol Acetate