The arrest of secretion response in yeast: signaling from the secretory path to the nucleus via Wsc proteins and Pkc1p.
The arrest of secretion response (ASR) in sec mutants reversibly inhibits nuclear import and relocates nuclear proteins to the cytoplasm. sec mutants also relocate nucleoporins; however, endocytic and Golgi-to-vacuole transport mutants do not cause relocation. The ASR requires Wsc membrane proteins that are trapped along the secretory path, rather than those which are at the plasma membrane. The activity of the downstream kinase, Pkc1p, is also required; however, the Pkc1p MAP kinase cascade is not. sec mutants initiate compensatory transcriptional changes distinct from those of the unfolded protein response.
Nanduri
J
J
Department of Pathology and Cell Biology Program, Case Western Reserve University, Cleveland, OH 44106, USA.
Tartakoff
A M
AM
D005656:Fungal Proteins / Q000378:metabolism*; D015151:Immunoblotting; D020104:Immunophilins / Q000378:metabolism; D047908:Intracellular Signaling Peptides and Proteins; D020935:MAP Kinase Signaling System / Q000502:physiology*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D022022:Nuclear Pore / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D011493:Protein Kinase C / Q000378:metabolism; D021381:Protein Transport / Q000502:physiology*; D011956:Receptors, Cell Surface / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D013696:Temperature; D015003:Yeasts / Q000502:physiology*
D005656:Fungal Proteins; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D009687:Nuclear Proteins; D011956:Receptors, Cell Surface; C119383:SLG1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; C120218:WSC2 protein, S cerevisiae; C097993:Pkc1 protein, Trichoderma reesei; D011493:Protein Kinase C; C403327:FPR3 protein, S cerevisiae; D020104:Immunophilins