[Increase in eremomycin production by regeneration and UV-irradiation of Amycolatopsis orientalis subsp. eremomycini protoplasts].
Protoplast regeneration of Amycolatopsis orientalis subsp. eremomycini producing eremomycin leads to the change of cultural and morphological properties as well as synthesis of secondary metabolites. Formation of plus-variants with enchanced antibiotic production was promoted by UV-irradiation of protoplasts. These plus-variants can be successfully used for repeating protoplasting--UV-irradiation of protoplasts with further increasing of the strain productivity. Finally activity of the initial A. orientalis culture was increased 7-8 times. Proposed method is recommended for the improvement of actinomycetes strains producing antibiotics especially in the case of cultures with poor sporulation.
Trenin
A S
AS
G. F. Gause Research Institute of New Antibiotics, Russian Academy of Medical Sciences, Moscow.
Fedorova
G B
GB
Laĭko
A V
AV
Dudnik
Iu V
IuV
D000192:Actinomycetales / Q000378:metabolism* / Q000528:radiation effects; D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D003470:Culture Media; D006020:Glycopeptides; D011523:Protoplasts / Q000378:metabolism* / Q000528:radiation effects; D014466:Ultraviolet Rays
D000900:Anti-Bacterial Agents; D003470:Culture Media; D006020:Glycopeptides; C053759:eremomycin