[Production of transgenic lamb integrated with modified human anti-trypsin gene].
This experiment is to produce the human mAAT(modified anti-trypsin) which cures the emphysema specifically through mammalian galactophore of transgenic goat. 56 goats were selected as donor for superovulation by FSF + LH microinjection in this experiment. The pronucleic embryos were injected with human mAAT gene after fertilization in vivo, and transferred to the donors or receptors directly. The superovulation was better in March and May than in December with the number of ovulation of 19.50, 21.70 and 16.06, and number of fertilized embryos of 4.31, 6.48 and 3.57 per-animal respectively. The pregnant rates were 18.18% and 25% respectively after transferred to donors and receptors with natural estrus. The donors also can be used as the embryo receptor with no remarkable decrease of pregnant rate. 29 lamb were labored. 4 positive transgenic lamb were checked by PCR, PCR-Southern and Southern analysis. The integrated efficiency of foreign DNA was 13.79% with microinjection of high copy number of foreign DNA fragment.
Lian
Z X
ZX
China Agricultural University, Beijing 100094, China. lianzhx@cau.edu.cn
Zhang
L
L
Liu
Z L
ZL
Pan
Q Z
QZ
Song
C Y
CY
Ding
X
X
Dai
Y P
YP
Li
N
N
Wu
C X
CX
Liu
Y H
YH
Ao
H
H
Shang
J H
JH
Chen
Y
Y
Tian
X J
XJ
Han
B
B
Chang
J H
JH
Li
Z Q
ZQ
Sang
Z Y
ZY
D000818:Animals; D030801:Animals, Genetically Modified; D005260:Female; D006041:Goats; D006801:Humans; D011247:Pregnancy; D019076:Transgenes; D014361:Trypsin Inhibitors / Q000235:genetics*
D014361:Trypsin Inhibitors