title: Systematic status of Hynobius tokyoensis (Amphibia: Urodela) from Aichi Prefecture, Japan: a biochemical survey.
abstract: Taxonomic status of a salamander now identified as Hynobius tokyoensis from Aichi Prefecture, Japan, was assessed by allozymic comparisons with its relatives. All three populations of H. tokyoensis from Aichi formed a monophyletic group with populations of H. nebulosus; this group is substantially diverged from a conspecific population from Kanagawa that formed another group with H. lichenatus. In the former group, the population of H. nebulosus from near the type locality was more divergent from the other conspecific populations than were three populations of H. tokyoensis from Aichi. It is quite clear that the salamander from Aichi now identified as H. tokyoensis is genetically much closer to H. nebulosus than to H. tokyoensis from Kanagawa, which is closest geographically to its type locality. This result conforms well to reported results of morphological and genetic studies, and the salamander from Aichi, now called H. tokyoensis, should be identified as H. nebulosus, which is sufficiently diverged from H. tokyoensis to be considered a distinct species.
authors.affiliation: Graduate School of Human and Environmental Studies, Kyoto University, Yoshida-Nihonmatsu-cho, Sakyo-ku, Kyoto, 606-8501 Japan. fumi@zoo.zool.kyoto-u.ac.jp
authors.forename: M
authors.initials: M
authors.lastname: Matsui
authors.forename: K
authors.initials: K
authors.lastname: Nishikawa
authors.forename: S
authors.initials: S
authors.lastname: Tanabe
authors.forename: Y
authors.initials: Y
authors.lastname: Misawa
mesh_terms: D000465:Algorithms; D000818:Animals; D004592:Electrophoresis, Starch Gel; D005787:Gene Frequency; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism*; D007564:Japan; D007814:Larva / Q000033:anatomy & histology*; D008099:Liver / Q000201:enzymology*; D010802:Phylogeny; D011110:Polymorphism, Genetic; D014562:Caudata / Q000145:classification* / Q000378:metabolism
chemical_list: D007527:Isoenzymes