Tandem repetition of baculovirus ie1 promoter results in upregulation of transcription.
In order to improve a virus-independent gene expression system in insect cells, the effect of the repetition of the promoter sequence of a baculovirus immediate early gene, BmNPV ie1, on the gene expression activity was examined. Tandem repetition of ie1 promoter resulted in about a 4-fold increase of the reporter gene expression activity. Primer extension analysis revealed that the repetition increased not only the amount of transcripts (4 to 5-times) but also the number of sites of transcription initiation (at least 3 extra initiation sites). A mutational experiment demonstrated the involvement of independent cis-acting elements in these two different promoter-enhancing effects.
Kojima
K
K
Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Sapporo, Japan.
Hayakawa
T
T
Asano
S
S
Bando
H
H
D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D001483:Base Sequence; D002461:Cell Line, Transformed; D015870:Gene Expression; D020869:Gene Expression Profiling; D017781:Genes, Immediate-Early / Q000235:genetics*; D007915:Lepidoptera / Q000166:cytology / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000032:analysis; D014158:Transcription, Genetic; D015854:Up-Regulation
D012333:RNA, Messenger