title: Mammalian cell cultivation in space.
abstract: Equipment used in space for the cultivation of mammalian cells does not meet the usual standard of earth bound bioreactors.  Thus, the development of a space worthy bioreactor is mandatory for two reasons: First, to investigate the effect on single cells of the space environment in general and microgravity conditions in particular, and second, to provide researchers on long term missions and the Space Station with cell material.  However, expertise for this venture is not at hand.  A small and simple device for animal cell culture experiments aboard Spacelab (Dynamic Cell Culture System; DCCS) was developed.  It provides 2 cell culture chambers, one is operated as a batch system, the other one as a perfusion system.  The cell chambers have a volume of 200 microliters.  Medium exchange is achieved with an automatic osmotic pump.  The system is neither mechanically stirred nor equipped with sensors.  Oxygen for cell growth is provided by a gas chamber that is adjacent to the cell chambers.  The oxygen gradient produced by the growing cells serves to maintain the oxygen influx by diffusion.  Hamster kidney cells growing on microcarriers were used to test the biological performance of the DCCS.  On ground tests suggest that this system is feasible.
authors.affiliation: Institute of Biotechnology, ETH-Hönggerberg, Zürich, Switzerland.
authors.forename: F K
authors.initials: FK
authors.lastname: Gmünder
authors.forename: R N
authors.initials: RN
authors.lastname: Suter
authors.forename: M
authors.initials: M
authors.lastname: Kiess
authors.forename: R
authors.initials: R
authors.lastname: Urfer
authors.lastname: Nordau C-G
authors.forename: A
authors.initials: A
authors.lastname: Cogoli
mesh_terms: D000818:Animals; D002452:Cell Count; D002455:Cell Division; D002478:Cells, Cultured; D006224:Cricetinae; D015194:Diffusion Chambers, Culture / Q000295:instrumentation*; D004867:Equipment Design; D007668:Kidney / Q000166:cytology* / Q000187:drug effects; D008647:Mesocricetus; D010100:Oxygen / Q000494:pharmacology; D013026:Space Flight / Q000295:instrumentation*; D014893:Weightlessness
chemical_list: D010100:Oxygen