Structure and properties of novel inclusions in Shewanella putrefaciens.
Cytoplasmic inclusions surrounded by a bilayer membrane were seen in thin sections. negatively stained and freeze-fractured preparations of Shewanella putrefaciens. Cells harvested from the late exponential and early stationary phase showed a higher number of these vesicles than bacteria isolated from early exponential or late stationary phase. Chemical dyes for polyphosphate or poly-beta-hydroxybutyrate did not stain the material enclosed within these vesicles. Elemental analysis of the material indicated that the content was organic in nature and might be a protein. HPLC analysis of the material showed that it was probably not a carbon source, nor an electron acceptor used by S. putrefaciens.
Krause
B
B
Center for Great Lakes Studies, University of Wisconsin-Milwaukee 53204, USA.
Beveridge
T J
TJ
Remsen
C C
CC
Nealson
K H
KH
D003470:Culture Media; D006092:Gram-Negative Facultatively Anaerobic Rods / Q000737:chemistry / Q000254:growth & development / Q000502:physiology / Q000648:ultrastructure*; D006885:Hydroxybutyrates / Q000032:analysis; D002479:Inclusion Bodies / Q000737:chemistry* / Q000502:physiology / Q000648:ultrastructure*; D008854:Microscopy, Electron; D011091:Polyesters / Q000032:analysis; D011122:Polyphosphates / Q000032:analysis; D000094122:Polyhydroxybutyrates
D003470:Culture Media; D006885:Hydroxybutyrates; D011091:Polyesters; D011122:Polyphosphates; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates