Clinical outcome of treatment cycles using preimplantation genetic diagnosis for structural chromosomal abnormalities.
OBJECTIVE
To explore oocyte recovery, embryo quality, the number of transferable embryos and pregnancy rate after preimplantation genetic diagnosis (PGD) in patients with structural chromosomal aberrations.


METHODS
PGD was performed in seven couples with Robertsonian translocations (Rob), eight couples with reciprocal translocations (Rec), two couples with inversions and one couple with a deletion. A total of 43 treatment cycles were carried out.


RESULTS
A total of 14.2 oocytes per cycle were retrieved. Fertilisation and cleavage rates were 63% and 58%, respectively. Of the biopsied embryos 20% were transferable. Comparison of the Rob and Rec group revealed no significant differences in number of oocytes, fertilisation or cleavage rates. The number of transferable embryos after biopsy was significantly higher in the Rob group than in the Rec group. When embryo transfer (ET) was performed the pregnancy rate did not differ between the Rob and the Rec groups. Twenty-eight embryo transfers (one or two embryos) were carried out leading to eight clinical pregnancies (29% per ET): two twins, four singletons, one miscarriage and one ectopic pregnancy. All the children are carriers of balanced chromosomal aberrations.


CONCLUSIONS
An acceptable pregnancy rate can be achieved among couples with structural chromosomal abnormalities.
Fridström
M
M
Department of Clinical Sciences, Division of Obstetrics and Gynecology, Karolinska Institute, K 57 Huddinge University Hospital, S-141 86 Stockholm, Sweden. margareta.fridstrom@obgyn.hs.sll.se
Ahrlund-Richter
L
L
Iwarsson
E
E
Malmgren
H
H
Inzunza
J
J
Rosenlund
B
B
Sjöblom
P
P
Nordenskjöld
M
M
Blennow
E
E
Hovatta
O
O
D000328:Adult; D002869:Chromosome Aberrations / Q000175:diagnosis*; D025063:Chromosome Disorders; D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro; D006579:Heterozygote; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008423:Maternal Age; D009865:Oocytes / Q000502:physiology; D011247:Pregnancy; D011256:Pregnancy Outcome; D018873:Pregnancy Rate; D018566:Pregnancy, High-Risk; D019836:Preimplantation Diagnosis