title: Protein binding of glufosinate and factors affecting it revealed by an equilibrium dialysis technique.
abstract: We investigated the protein binding of glufosinate ammonium (GLF) and several factors affecting this binding using human serum albumin (HSA) and human volunteer serum under various conditions. The mean ratios of the free GLF (RFr-GLF) to 4% HSA were examined in the sera of patients described elsewhere at GLF levels from 1 microg/mL to 500 microg/mL; the range was found to be only from 0.80 to 0.88. Neither the incubation temperature nor buffers containing different chloride ion concentrations had any effect on the RFr-GLF to HSA. Moreover, the addition of heparin, glycoprotein-alpha1-acid (AAG), and sodium azide had no effect on the RFr-GLF. However, pH of the isotonic phosphate buffer and the addition of palmitic or oleic acid were seen to have an effect. In this study, the mean RFr-GLF to healthy human serum was 0.99. This high value was evidenced that GLF was rapidly excreted through the renal route.
authors.affiliation: Department of Analytical Chemistry, Niigata College of Pharmacy, Japan. horiy@med.niigata-u.ac.jp
authors.forename: Y
authors.initials: Y
authors.lastname: Hori
authors.forename: K
authors.initials: K
authors.lastname: Koyama
authors.forename: M
authors.initials: M
authors.lastname: Fujisawa
authors.forename: M
authors.initials: M
authors.lastname: Nakajima
authors.forename: K
authors.initials: K
authors.lastname: Shimada
authors.forename: Y
authors.initials: Y
authors.lastname: Hirose
authors.forename: Y
authors.initials: Y
authors.lastname: Kohda
authors.forename: H
authors.initials: H
authors.lastname: Akuzawa
mesh_terms: D000328:Adult; D000613:Aminobutyrates / Q000737:chemistry / Q000378:metabolism* / Q000493:pharmacokinetics; D006540:Herbicides / Q000737:chemistry / Q000378:metabolism* / Q000493:pharmacokinetics; D006801:Humans; D006863:Hydrogen-Ion Concentration; D019301:Oleic Acid / Q000737:chemistry; D019308:Palmitic Acid / Q000737:chemistry; D011485:Protein Binding; D013048:Specimen Handling; D013696:Temperature
chemical_list: D000613:Aminobutyrates; D006540:Herbicides; D019301:Oleic Acid; D019308:Palmitic Acid; C003121:phosphinothricin