Gene C2 of the monopartite geminivirus tomato yellow leaf curl virus-China encodes a pathogenicity determinant that is localized in the nucleus.
Expression of the Tomato yellow leaf curl virus-China (TYLCV-C) C2 protein and green fluorescent protein (GFP) fused to the C2 protein (C2-GFP) in Nicotiana benthamiana from a Potato virus X (PVX) vector induced necrotic ringspots on inoculated leaves as well as necrotic vein banding and severe necrosis on systemically infected leaves. The localization of GFP fluorescence in plant cells infected with PVX/C2-GFP and in insect cells transfected with Baculovirus expressing C2-GFP indicates that the TYLCV-C C2 protein is capable of shuttling GFP into plant and insect cell nuclei. Our data demonstrate that the TYLCV-C C2 protein may contribute to viral pathogenicity in planta and is nuclear localized.
van Wezel
R
R
Department of Entomology and Plant Pathology, Horticulture Research International, Kent, UK.
Liu
H
H
Tien
P
P
Stanley
J
J
Hong
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000821:virology; D017791:Geminiviridae / Q000235:genetics* / Q000472:pathogenicity*; D005814:Genes, Viral; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000235:genetics; D018551:Solanum lycopersicum / Q000821:virology*; D008969:Molecular Sequence Data; D030821:Plants, Genetically Modified; D017863:Potexvirus / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics; D018411:Spodoptera; D014026:Nicotiana / Q000821:virology; D014162:Transfection; D014764:Viral Proteins / Q000235:genetics; D014774:Virulence / Q000235:genetics
D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins; D049452:Green Fluorescent Proteins