title: Structure of amyloplasts and endoplasmic reticulum in the root caps of Lepidium sativum and Zea mays observed after selective membrane staining and by high-voltage electron microscopy.
abstract: The structure of plastids in the root cap of cress and maize was studied by low- and high-voltage electron microscopy after staining their membranes with a mixture of zinc iodide and osmium tetroxide. In plastids of both species electron-opaque membranes were found in the plastid interior while membranes of lesser electron-opacity comprised the outer envelope and vesicles and cisternae underlying it. Electron-opaque tubules, often in groups attached to the inner membrane of the amyloplast envelope, were found in cress but not in maize. The internal, less-opaque membranes were often found associated with the starch grains. No specific association could be seen between amyloplasts and endoplasmic reticulum (ER); their surfaces showed no regular contact or connexion, though the amyloplasts clearly indented the underlying ER. The ER in statocytes was predominantly tubular in cress but predominantly cisternal in maize.
authors.affiliation: Agricultural Research Council Letcombe Laboratory, Wantage, UK.
authors.forename: P W
authors.initials: PW
authors.lastname: Barlow
authors.forename: C R
authors.initials: CR
authors.lastname: Hawes
authors.forename: J C
authors.initials: JC
authors.lastname: Horne
mesh_terms: D019607:Brassicaceae / Q000166:cytology / Q000648:ultrastructure*; D004396:Coloring Agents; D004721:Endoplasmic Reticulum / Q000648:ultrastructure*; D018466:Gravity Sensing / Q000502:physiology*; D008566:Membranes / Q000737:chemistry; D018519:Meristem / Q000648:ultrastructure; D008854:Microscopy, Electron; D009993:Osmium Tetroxide; D018518:Plant Root Cap / Q000502:physiology / Q000648:ultrastructure*; D018087:Plastids / Q000648:ultrastructure*; D013194:Staining and Labeling; D013213:Starch / Q000032:analysis; D003313:Zea mays / Q000166:cytology / Q000648:ultrastructure*; D017967:Zinc Compounds
chemical_list: D004396:Coloring Agents; D017967:Zinc Compounds; C035252:osmium zinc iodide; D013213:Starch; D009993:Osmium Tetroxide