title: Sequence analysis of env C2/V3, gag p17/p24, and pol protease regions of 25 HIV type 1 isolates from Ho Chi Minh City, Vietnam.
abstract: Env C2/V3, gag p17/p24, pol protease, and RT regions of HIV-1 isolates recently obtained from 25 HIV-1 seropositive individuals from Ho Chi Minh City (Vietnam) were studied, and genes subtypes were determined by DNA sequence analyses. Twenty-three isolates out of 25 were identified as belonging to subtype E, now recognized as circulating recombinant form 1 (CRF01_AE). The motif at the top of the V3 loop (generally GPGQ) was then preceded by an isoleucine or a methionine (M) residue; the M residue might be a local signature of Vietnamese E isolates compared to Thai E viruses. Two isolates (8%) were shown to be intersubtype recombinants: one E/B and one CRF02_AG(IBNG)/D. The polymorphism of pol protease was considered only for CRF01_AE isolates and is clearly different from that recorded for B viruses with substitutions at positions 13, 35, 36, 41, 69, and 89.
authors.affiliation: Laboratoire de Virologie, Université Victor Ségalen Bordeaux 2, Bordeaux, France.
authors.forename: A
authors.initials: A
authors.lastname: Caumont
authors.forename: N T
authors.initials: NT
authors.lastname: Lan
authors.forename: N T
authors.initials: NT
authors.lastname: Uyen
authors.forename: P V
authors.initials: PV
authors.lastname: Hung
authors.forename: E
authors.initials: E
authors.lastname: Schvoerer
authors.forename: M S
authors.initials: MS
authors.lastname: Urriza
authors.forename: P
authors.initials: P
authors.lastname: Roques
authors.forename: M H
authors.initials: MH
authors.lastname: Schrive
authors.forename: T T
authors.initials: TT
authors.lastname: Lien
authors.forename: M E
authors.initials: ME
authors.lastname: Lafon
authors.forename: D
authors.initials: D
authors.lastname: Dormont
authors.forename: F
authors.initials: F
authors.lastname: Barre-Sinoussi
authors.forename: H J
authors.initials: HJ
authors.lastname: Fleury
mesh_terms: D000293:Adolescent; D000328:Adult; D000595:Amino Acid Sequence; D005260:Female; D015686:Gene Products, env / Q000737:chemistry / Q000235:genetics; D015752:Genes, env / Q000235:genetics*; D015750:Genes, gag / Q000235:genetics*; D015751:Genes, pol / Q000235:genetics*; D015658:HIV Infections / Q000453:epidemiology* / Q000821:virology*; D015497:HIV-1 / Q000737:chemistry / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D011110:Polymorphism, Genetic / Q000235:genetics; D016415:Sequence Alignment; D014744:Vietnam / Q000453:epidemiology
chemical_list: D015686:Gene Products, env