title: Dual mode of gating of voltage-dependent anion channel as revealed by phosphorylation.
abstract: The single-channel electrophysiological properties of the voltage-dependent anion channel (VDAC) of mitochondria from rat liver have been investigated under normal and phosphorylated (with protein kinase A) conditions. Experimental observations show that phosphorylation does not affect the current level and the opening probability in the positive clamping potentials, but leads to lowering of current magnitude and opening probability in the negative clamping potentials. The opening probability versus voltage (V) plot for native VDAC fits a Gaussian function symmetric around V = 0, whereas the same for phosphorylated VDAC fits a linear combination of two Gaussian functions. This indicates that there are two gating modes of VDAC; the negative voltage sensor (gate) undergoes modification due to phosphorylation.
authors.affiliation: Department of Biophysics, University of Delhi South Campus, Benito Juarez Road, New Delhi, 110021, India.
authors.forename: A K
authors.initials: AK
authors.lastname: Bera
authors.forename: S
authors.initials: S
authors.lastname: Ghosh
mesh_terms: D000200:Action Potentials / Q000502:physiology; D000818:Animals; D000838:Anions; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism*; D004553:Electric Conductivity; D004594:Electrophysiology; D015640:Ion Channel Gating / Q000502:physiology; D007473:Ion Channels / Q000378:metabolism*; D007700:Kinetics; D008051:Lipid Bilayers; D008433:Mathematics; D008565:Membrane Proteins / Q000378:metabolism*; D008567:Membranes, Artificial; D008930:Mitochondria, Liver / Q000378:metabolism*; D018408:Patch-Clamp Techniques; D010766:Phosphorylation; D018272:Porins / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats; D050994:Voltage-Dependent Anion Channels
chemical_list: D000838:Anions; D007473:Ion Channels; D008051:Lipid Bilayers; D008565:Membrane Proteins; D008567:Membranes, Artificial; D018272:Porins; D050994:Voltage-Dependent Anion Channels; D017868:Cyclic AMP-Dependent Protein Kinases