Orientation and dynamics of an antimicrobial peptide in the lipid bilayer by solid-state NMR spectroscopy.
The orientation and dynamics of an 18-residue antimicrobial peptide, ovispirin, has been investigated using solid-state NMR spectroscopy. Ovispirin is a cathelicidin-like model peptide (NH(2)-KNLRRIIRKIIHIIKKYG-COOH) with potent, broad-spectrum bactericidal activity. (15)N NMR spectra of oriented ovispirin reconstituted into synthetic phospholipids show that the helical peptide is predominantly oriented in the plane of the lipid bilayer, except for a small portion of the helix, possibly at the C-terminus, which deviates from the surface orientation. This suggests differential insertion of the peptide backbone into the lipid bilayer. (15)N spectra of both oriented and unoriented peptides show a reduced (15)N chemical shift anisotropy at room temperature compared with that of rigid proteins, indicating that the peptide undergoes uniaxial rotational diffusion around the bilayer normal with correlation times shorter than 10(-4) s. This motion is frozen below the gel-to-liquid crystalline transition temperature of the lipids. Ovispirin interacts strongly with the lipid bilayer, as manifested by the significantly reduced (2)H quadrupolar splittings of perdeuterated palmitoyloleoylphosphatidylcholine acyl chains upon peptide binding. Therefore, ovispirin is a curved helix residing in the membrane-water interface that executes rapid uniaxial rotation. These structural and dynamic features are important for understanding the antimicrobial function of this peptide.
Yamaguchi
S
S
Department of Chemistry, Iowa State University, Ames, Iowa 50011, USA.
Huster
D
D
Waring
A
A
Lehrer
R I
RI
Kearney
W
W
Tack
B F
BF
Hong
M
M
D023181:Antimicrobial Cationic Peptides / Q000737:chemistry*; D001665:Binding Sites; D004058:Diffusion; D008051:Lipid Bilayers / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008567:Membranes, Artificial; D008956:Models, Chemical; D010743:Phospholipids / Q000737:chemistry*; D012399:Rotation; D013499:Surface Properties; D013816:Thermodynamics; D014867:Water / Q000737:chemistry
D023181:Antimicrobial Cationic Peptides; D008051:Lipid Bilayers; D008567:Membranes, Artificial; D010743:Phospholipids; C440189:ovispirin; D014867:Water