Electroinduced extraction of beta-galactosidase from Kluyveromyces lactis.
A new methodology for the extraction of beta-galactosidase from the yeast Kluyveromyces lactis was obtained by electropulsation. The application of a series of electric pulses (2 ms duration, 1 Hz frequency, and 4-4.5 kV/cm field strength) to fresh cells suspended in deionized water, followed by incubation in PBS, led to a spontaneous slow release of enzyme at a yield of 75-80% without any further treatment. Most of the enzyme was extracted within 8 h after electropulsation. This release was dependent on the growth phase. The specific activity of beta-galactosidase in the supernatant of pulsed cells was higher by a factor of 1.5-1.7 in comparison with crude extract.
Ganeva
V
V
Faculty of Biology, University of Sofia, Bulgaria.
Galutzov
B
B
Eynard
N
N
Teissié
J
J
D001709:Biotechnology / Q000379:methods; D004574:Electromagnetic Fields; D004790:Enzyme Induction; D007716:Kluyveromyces / Q000201:enzymology* / Q000254:growth & development; D001616:beta-Galactosidase / Q000096:biosynthesis* / Q000302:isolation & purification*
D001616:beta-Galactosidase