In vitro study on thiabendazole action on viability of Ascaris lumbricoides (Lineu, 1758) eggs.
The in vitro activity of thiabendazole on Ascaris lumbricoides eggs, which were recovered from uteri of worm excreted after chemotherapeutic treatment, was studied. Four concentrations of the drug were used: 1 - 2.5 - 5 - and 10 ppm during 24, 48 and 72 hours of exposure. Subsequently, the eggs were centrifuged, washed three times and H(2)SO(4)0.1N was added. The eggs were maintained in an incubator for 20 days at 28 degrees C. Finally, the percentage of embryonated eggs was determined under a lightmicroscope at a 100X magnification. After 48 and 72 hours of thiabendazole exposure, at a concentration of 10ppm, the drug showed complete inhibition of egg embryonation.
Massara
C L
CL
Laboratório de Helmintoses Intestinais, Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz, Belo Horizonte, MG, Brasil. massara@cpqrr.fiocruz.br
Ferreira
R S
RS
Guerra
H L
HL
Carvalho
O dos S
Odos S
D000818:Animals; D000969:Antinematodal Agents / Q000494:pharmacology*; D017164:Ascaris lumbricoides / Q000187:drug effects*; D010270:Parasite Egg Count; D013827:Thiabendazole / Q000494:pharmacology*
D000969:Antinematodal Agents; D013827:Thiabendazole