title: Effects of carbon ions on primary cultures of mouse brain cells.
abstract: Primary mixed cultures of astrocytes and microglia were obtained from neonatal mice, and were irradiated with high-LET carbon ions. Immunohistochemical staining showed astrocytes survived more prominently than microglia. Tagged with specific antibodies, astrocytes and microglia surviving after irradiation were counted by flow cytometry. Decreases in the number of microglia and astrocytes were detected at a dose as small as 2 Gy when Day 5 cultures were irradiated with 13 keV/micrometer carbon ions. When the cultures were irradiated on Day 10, the dose-dependent decrease of microglia was more prominent for 13 keV/micrometer carbon ions than 70 keV/micrometer carbon ions. Astrocytes showed a marginal decrease at Day 10 and Day 14. We concluded that microglia are more sensitive than astrocytes to carbon ions and X-rays, and that the radiosensitivity of microglia depends on both differentiation/proliferation status and radiation quality.
authors.affiliation: Space and Particle Radiation Science Research Group, National Institute of Radiological Sciences, Chiba, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Nojima
authors.forename: K
authors.initials: K
authors.lastname: Ando
authors.forename: H
authors.initials: H
authors.lastname: Fujiwara
authors.forename: S
authors.initials: S
authors.lastname: Ando
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001253:Astrocytes / Q000166:cytology / Q000528:radiation effects*; D001921:Brain / Q000166:cytology / Q000528:radiation effects; D002244:Carbon; D002452:Cell Count; D002478:Cells, Cultured / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D005434:Flow Cytometry; D020450:Heavy Ions; D007150:Immunohistochemistry; D018499:Linear Energy Transfer; D051379:Mice; D008809:Mice, Inbred C3H; D017628:Microglia / Q000166:cytology / Q000528:radiation effects*; D010315:Particle Accelerators; D011829:Radiation Dosage
chemical_list: D002244:Carbon