Characterization, physical location and expression of the genes encoding calcium/calmodulin-dependent protein kinases in maize (Zea mays L.).
The maize genomic sequence and cDNA encoding a calcium/calmodulin-dependent protein kinase homolog were isolated and identified. The deduced peptide (MCK2) from this cDNA shared high amino acid identity (91.2%) with maize MCK1. These two genes were physically mapped onto chromosomes by fluorescence in situ hybridization using the first introns of the genes as gene-specific probes. While the MCK1 gene was assigned to a locus on the long arm of chromosome 9, the MCK2 gene was localized to a locus on the long arm of chromosome 1. Both of these genes were expressed in roots, leaves, stems and flowers, and the expression patterns of MCK were verified by RNA in situ hybridization. These results indicated that MCK expression is temporally and spatially regulated during maize growth and development.
Wang
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Key Laboratory of MOE for Plant Developmental Biology, Wuhan University, China.
Liang
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S
Lu
Y T
YT
D000595:Amino Acid Sequence; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D002874:Chromosome Mapping; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D038362:Glycogen Synthase Kinase 3; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D018514:Plant Structures / Q000201:enzymology / Q000235:genetics; D011505:Protein-Tyrosine Kinases / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins; D017386:Sequence Homology, Amino Acid; D003313:Zea mays / Q000201:enzymology / Q000235:genetics*
D012333:RNA, Messenger; D029701:Saccharomyces cerevisiae Proteins; D011505:Protein-Tyrosine Kinases; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D038362:Glycogen Synthase Kinase 3; C068124:MCK1 protein, S cerevisiae