Antimutagenic effect of Agaricus blazei Murrill mushroom on the genotoxicity induced by cyclophosphamide.
Agaricus blazei Murrill extracts have previously been shown to have anticarcinogenic and antimutagenic properties. These results suggest that antimutagenic activity, besides the modulation of the immune system, might be involved in the anticarcinogenic action of A. blazei. To investigate the possible antimutagenic effect of A. blazei in vivo, we evaluated its effect on clastogenicity induced by cyclophosphamide (CP) in mice, using the micronucleus test in bone marrow (MNPCE) and in peripheral blood (MNRET). Male Swiss mice were treated with CP (25 or 50mg/kg i.p.) or with CP plus mushroom solution at three different temperatures: 4, 21, and 60 degrees C. Aqueous solution of a mixture from various lineages of the mushroom inhibited induction of micronuclei by CP in bone marrow and in peripheral blood of mice. In contrast to the mixture of lineages, a single isolated lineage did not lead to a reduction of CP-induced MN frequencies in either bone marrow or blood cells of mice. The results suggest that under certain circumstances these mushrooms exhibit antimutagenic activities that might contribute to an anticarcinogenic effect.
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Departamento de Patologia, Faculdade de Medicina, UNESP, 18618-000, SP, Botucatu, Brazil.
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D000364:Agaricus / Q000737:chemistry*; D000818:Animals; D016587:Antimutagenic Agents / Q000494:pharmacology*; D001854:Bone Marrow Cells / Q000187:drug effects / Q000473:pathology; D003520:Cyclophosphamide / Q000633:toxicity; D004249:DNA Damage / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D051379:Mice; D015162:Micronucleus Tests; D009153:Mutagens / Q000633:toxicity; D010936:Plant Extracts / Q000494:pharmacology*; D012156:Reticulocytes / Q000187:drug effects / Q000473:pathology
D016587:Antimutagenic Agents; D009153:Mutagens; D010936:Plant Extracts; D003520:Cyclophosphamide