title: The effects of space flight on the contractile apparatus of antigravity muscles:  implications for aging and deconditioning.
abstract: Previous studies have shown that the unloading of skeletal muscle, as occurring during exposure to space flight, exerts a profound effect on both the mass (cross sectional area) of skeletal muscle fibers and the relative expression of protein isoforms comprising the contractile system.  Available information suggests that slow (type I) fibers, comprising chiefly the antigravity muscles of experimental animals, in addition to atrophying, undergo alterations in the type of myosin heavy chain (MHC) expressed such that faster isoforms become concomitantly expressed in a sub-population of slow fibers when insufficient force-bearing activity is maintained on the muscle.  Consequently, these transformations in both mass and myosin heavy chain phenotype could exert a significant impact on the functional properties of skeletal muscle as manifest in the strength, contractile speed, and endurance scope of the muscle.  To further explore these issues, a study was performed in which young adult male rats were exposed to zero gravity for six days, following which, the antigravity soleus muscle was examined for a) contractile properties, determined in situ and b) isomyosin expression, as studied using biochemical, molecular biology, and histochemical/immunohistochemical techniques.
authors.affiliation: Department of Physiology and Biophysics, University of California, Irvine 92717, USA.
authors.forename: K M
authors.initials: KM
authors.lastname: Baldwin
authors.forename: V J
authors.initials: VJ
authors.lastname: Caiozzo
authors.forename: F
authors.initials: F
authors.lastname: Haddad
authors.forename: M J
authors.initials: MJ
authors.lastname: Baker
authors.forename: R E
authors.initials: RE
authors.lastname: Herrick
mesh_terms: D000375:Aging / Q000502:physiology*; D000818:Animals; D007150:Immunohistochemistry; D007536:Isomerism; D008297:Male; D009119:Muscle Contraction / Q000502:physiology*; D018656:Muscle Fibers, Fast-Twitch / Q000378:metabolism / Q000473:pathology / Q000502:physiology; D018657:Muscle Fibers, Slow-Twitch / Q000378:metabolism / Q000473:pathology / Q000502:physiology; D018482:Muscle, Skeletal / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D009133:Muscular Atrophy / Q000378:metabolism / Q000503:physiopathology*; D018995:Myosin Heavy Chains / Q000032:analysis / Q000737:chemistry / Q000235:genetics; D010641:Phenotype; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013026:Space Flight; D014893:Weightlessness
chemical_list: D012333:RNA, Messenger; D018995:Myosin Heavy Chains