Antisense properties of 2'-O-dimethylaminooxyethyl (2'-O-DMAOE) oligonucleotides.
Antisense oligonucleotides with 2'-O-(2-[N,N-dimethyl)aminooxy]ethyl) or (2'-O-DMAOE) modification were synthesized and evaluated for nuclease resistance and pharmacology both in vitro and in vivo. This modification exhibits very high nuclease resistance and efficacy in various biological (ICAM-1, C-raf and PKC-alpha) targets.
Prakash
T P
TP
Isis Pharmaceuticals, 2292 Faraday Ave., Carlsbad, California 92008, USA.
Kawasaki
A M
AM
Johnston
J F
JF
Graham
M J
MJ
Condon
T P
TP
Manoharan
M
M
D000818:Animals; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism; D005260:Female; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D016376:Oligonucleotides, Antisense / Q000138:chemical synthesis* / Q000235:genetics / Q000494:pharmacology*; D009943:Organophosphorus Compounds / Q000138:chemical synthesis; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D016914:Ribonuclease H / Q000235:genetics
D016376:Oligonucleotides, Antisense; D009943:Organophosphorus Compounds; D012333:RNA, Messenger; C434331:phosphoramidite; D018799:Intercellular Adhesion Molecule-1; D016914:Ribonuclease H