Toti-/pluripotential stem cells and epigenetic modifications.
The recent fascinating breakthrough in the area of stem cell research is the successful production of cloned animals via nuclear transplantation of somatic nucleus by intrinsic trans-acting factors of oocytes and trans-differentiation of somatic stem cells from adult organs induced by extrinsic growth factors. During the process of nuclear reprogramming, epigenetic modification of the somatic nuclei must be achieved to acquire toti-/pluripotential competence. However, the molecular mechanism involved is largely unknown. It has been shown that DNA methylation, histone acetylation and chromatin structure are involved in the establishment of epigenetic modification. Now it is evident that they function cooperatively to establish and maintain active or inactive chromatin state. Here we discuss the mechanisms of epigenetic modification potentially involved in the event of nuclear reprogramming.
Tada
T
T
Department of Development and Differentiation, Institute for Frontier Medical Sciences, Kyoto University, Japan. ttada@frontier.kyoto-u.ac.jp
Tada
M
M
D000818:Animals; D002454:Cell Differentiation; D002843:Chromatin / Q000235:genetics; D019175:DNA Methylation; D004622:Embryo, Mammalian / Q000502:physiology*; D005854:Germ Cells / Q000502:physiology*; D006801:Humans; D006822:Hybrid Cells; D008954:Models, Biological; D053652:Nuclear Transfer Techniques; D013234:Stem Cells / Q000502:physiology*
D002843:Chromatin