title: Intradiskal electrothermal therapy: a preliminary histologic study.
abstract: OBJECTIVE
  To characterize descriptively the histologic and temperature effects of intradiskal electrothermal annuloplasty on human cadaveric lumbar disks.
  
  
  METHODS
  In vitro histologic study.
  
  
  METHODS
  Hospital-based soft-tissue research laboratory.
  
  
  METHODS
  Six human cadaveric lumbar disks, from 5 cadavers aged 39 to 79 who died from nonspine-related causes.
  
  
  METHODS
  Intradiskal electrothermal therapy (IDET) by using a standard high-temperature heating protocol with the temperature of the probe gradually increased from 65 degrees C to 90 degrees C over 16.5 minutes. Disks were stained and examined by light microscopy and electron microscopy.
  
  
  METHODS
  Temperatures in outer annulus, gross macroscopic changes, and histologic damage.
  
  
  RESULTS
  Gross inspection showed a small circumferential area of tissue alteration localized to the posterior annulus but not extending to the endplates. Light microscopy of the posterior aspect of the lumbar disks showed denaturation, shrinkage, and coalescence of annular collagen; the anterior portions, which served as internal controls, showed no evidence of damage. The endplates were structurally preserved and showed no evidence of damage. Electron microscopy showed extensive collagen disorganization, decreased quantity of collagen, collagen fibril shrinkage, and chondrocyte damage when compared with a control portion. The temperature curves showed parallel changes in temperature at the level of the probe and at the posterior portion of the disk.
  
  
  CONCLUSIONS
  IDET raises temperatures sufficiently to induce collagen denaturation and coalescence. These histologic changes may play a substantial role in the clinical efficacy of IDET.
authors.affiliation: Physiatry Service, Hospital for Special Surgery, New York, NY 10021, USA.
authors.forename: R V
authors.initials: RV
authors.lastname: Shah
authors.forename: G E
authors.initials: GE
authors.lastname: Lutz
authors.forename: J
authors.initials: J
authors.lastname: Lee
authors.forename: S B
authors.initials: SB
authors.lastname: Doty
authors.forename: S
authors.initials: S
authors.lastname: Rodeo
mesh_terms: D000328:Adult; D000368:Aged; D001831:Body Temperature; D002102:Cadaver; D019902:Chondrocytes / Q000648:ultrastructure; D002908:Chronic Disease; D003094:Collagen / Q000648:ultrastructure; D004564:Electrocoagulation / Q000009:adverse effects / Q000295:instrumentation / Q000379:methods / Q000592:standards*; D006801:Humans; D007403:Intervertebral Disc / Q000601:surgery* / Q000648:ultrastructure*; D017116:Low Back Pain / Q000209:etiology / Q000473:pathology* / Q000503:physiopathology / Q000601:surgery*; D008854:Microscopy, Electron; D008875:Middle Aged; D013997:Time Factors
chemical_list: D003094:Collagen