title: Haemocompatibility of endovascular coronary stents: Wiktor GX.
abstract: The stent to be examined (Wiktor-Stent, Medtronic ESTC, Kerkrade, NL) was mounted into a closed-loop tubular-system and perfused with platelet-rich plasma (PRP). As controls the tubular-system without stent (as non-thrombogenic control) and secondly the tube filled with glassbeads (as thrombogenic control) were evaluated. A decrease in the number of singularly circulating thrombocytes correlated well with an increases in circulating platelet aggregates. The increasing activation of thrombocytes was demonstrated by immunolabelling of surface structures (CD 62) which become prominent on activation of thrombocytes. The increase in case of the non-thrombogenic controls is thought to be due to the action of the roller-pump. This increase was coincident with an increase in immunologically labelled GPIIb/IIIa receptors and well correlated with an increase in platelet activation as demonstrated by the elevated CD 62 label. In spite of the use of anticoagulation principles in the perfusion model, thrombin was generated (measured by the TAT-complex) in all three cases and the completed coagulation (measured by the occurrence of fibrin D-dimers) also happened. The amount of D-dimers was small, however, in the cases of non-thrombogenic controls and of tubes equipped with stents. Only after the contact of PRP with tubes filled with glass-beads a significant increase in D-dimers followed. In conclusion the implantation of a stent led to an activation, adherence and aggregation of thrombocytes to a somewhat greater extent as in the control-system. It has, however, a much less thrombogenic surface than glass-beads.
authors.affiliation: Dresdner Institut für Herz- und Kreislaufforschung, Dresden. dihkf@saarmail.de
authors.forename: F
authors.initials: F
authors.lastname: Jung
authors.forename: R
authors.initials: R
authors.lastname: Bach
authors.forename: C
authors.initials: C
authors.lastname: Mrowietz
authors.forename: U
authors.initials: U
authors.lastname: Seyfert
authors.forename: R P
authors.initials: RP
authors.lastname: Franke
mesh_terms: D000328:Adult; D015906:Angioplasty, Balloon, Coronary / Q000295:instrumentation*; D005260:Female; D005338:Fibrin Fibrinogen Degradation Products / Q000378:metabolism; D006801:Humans; D008297:Male; D008422:Materials Testing; D008955:Models, Cardiovascular; D015539:Platelet Activation / Q000502:physiology*; D010974:Platelet Aggregation / Q000502:physiology*; D015607:Stents; D013917:Thrombin / Q000378:metabolism; D013927:Thrombosis / Q000097:blood*
chemical_list: D005338:Fibrin Fibrinogen Degradation Products; C036309:fibrin fragment D; D013917:Thrombin