title: Biochemical-genetic characterization of the chromosomally encoded extended-spectrum class A beta-lactamase from Rahnella aquatilis.
abstract: From whole-cell DNA of a clinical isolate of the enterobacterial species Rahnella aquatilis, a beta-lactamase gene was cloned that encoded a chromosomally encoded Ambler class A enzyme, RAHN-1. RAHN-1, with a pI of 7.2, shares 76, 73, and 71% amino acid identity with the extended-spectrum beta-lactamase of chromosomal origin from Serratia fonticola and with the plasmid-mediated beta-lactamases CTX-M-2 and CTX-M-1, respectively. The hydrolysis spectrum of the clavulanic acid-inhibited RAHN-1 was expanded to cephalosporins such as cefuroxime, cefotaxime, and ceftriaxone, but not to ceftazidime. Its expression was not inducible.
authors.affiliation: Service de Bactériologie-Virologie, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris-Sud, 78 rue de Général Leclerc, 94275 Le Kremlin-Bicêtre Cedex, France.
authors.forename: S
authors.initials: S
authors.lastname: Bellais
authors.forename: L
authors.initials: L
authors.lastname: Poirel
authors.forename: N
authors.initials: N
authors.lastname: Fortineau
authors.forename: J W
authors.initials: JW
authors.lastname: Decousser
authors.forename: P
authors.initials: P
authors.lastname: Nordmann
mesh_terms: D000595:Amino Acid Sequence; D002511:Cephalosporins / Q000378:metabolism; D002876:Chromosomes, Bacterial; D003001:Cloning, Molecular; D006801:Humans; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D020638:Rahnella / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D001618:beta-Lactamases / Q000235:genetics* / Q000378:metabolism; D047090:beta-Lactams / Q000494:pharmacology
chemical_list: D002511:Cephalosporins; D047090:beta-Lactams; D001618:beta-Lactamases