title: Biosurfactant-rhamnolipid effects on yeast cells.
abstract: OBJECTIVE
  The aim of this work was to study the effect of the novel surfactant PS from Pseudomonas sp. S-17 on Saccharomyces cerevisiae 83-20 yeast cells and to compare it with the effect of the well known surfactant Triton X-100.
  
  
  RESULTS
  The effect of surfactants was investigated on the cells during growth, and on the separated cells. The cell-permeabilizing effect of surfactants was studied by following the release of protein and some enzyme activities. The biosurfactant did not affect the culture growth kinetics, and altered the polypeptide profiles of cells and membrane proteins in the same way as Triton X-100.
  
  
  CONCLUSIONS
  Results of this study demonstrate that biosurfactant PS and Triton X-100 have a similar type of action, mainly surface located, and that they do not affect the intracellular structures of yeast cells.
  
  
  CONCLUSIONS
  A novel surfactant PS was isolated from Pseudomonas sp. S-17. A mild effect of PS on yeast cells was demonstrated. The results indicate the ecological safety of the biosurfactant and its potential use in the development of environmentally-benign and efficient cleaning technologies.
authors.affiliation: Department of Microbial Biochemistry and Biosynthesis, Institute of Microbiology, Bulgarian Academy of Sciences, Acad. G. Bonchev str., bl. 26, 1113 Sofia, Bulgaria.
authors.forename: E
authors.initials: E
authors.lastname: Vasileva-Tonkova
authors.forename: D
authors.initials: D
authors.lastname: Galabova
authors.forename: E
authors.initials: E
authors.lastname: Karpenko
authors.forename: A
authors.initials: A
authors.lastname: Shulga
mesh_terms: D002463:Cell Membrane Permeability / Q000187:drug effects; D006017:Glycolipids / Q000494:pharmacology*; D017830:Octoxynol / Q000494:pharmacology; D021381:Protein Transport / Q000187:drug effects; D011550:Pseudomonas aeruginosa; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D013501:Surface-Active Agents / Q000494:pharmacology*
chemical_list: D006017:Glycolipids; D029701:Saccharomyces cerevisiae Proteins; D013501:Surface-Active Agents; C418382:rhamnolipid; D017830:Octoxynol