title: Alterations in adenylate ratios in plant cells after accelerated ion irradiation.
abstract: Levels of adenylate metabolism have been studied in cells of Nicotiana tabacum growing in vitro, and in root apex extracts of Pisum sativum irradiated at the 95-in. isochronous cyclotron U-240, Institute for Nuclear Research, Ukrainian National Academy of Sciences, Kyiv.  Particle beams of accelerated helium ions with energy 9.34 keV/micrometer were used.  Replacement and rapid freezing of the irradiated plants samples in liquid nitrogen were carried out with a manipulator and a remote control system.  After doses of 5, 20, 50, and 100 Gy of gamma-irradiation, as well as 50 and 100 Gy 4He irradiation, the cellular ATP/ADP ratio increased during early stages of the response.  This effect was absent at higher doses and after exposure to sparesly-ionizing radiation, when a rapid decline in the cellular ATP concentration and the ATP/ADP ratio occurred.
authors.affiliation: Institute of Botany, Ukranian National Academy of Sciences, Kyiv, Ukraine.
authors.forename: A
authors.initials: A
authors.lastname: Vasilenko
authors.forename: P G
authors.initials: PG
authors.lastname: Sidorenko
mesh_terms: D000244:Adenosine Diphosphate / Q000378:metabolism* / Q000528:radiation effects; D000255:Adenosine Triphosphate / Q000378:metabolism* / Q000528:radiation effects; D003037:Cobalt Radioisotopes; D017357:Cyclotrons; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays; D006371:Helium; D007477:Ions; D008854:Microscopy, Electron; D008928:Mitochondria / Q000528:radiation effects / Q000648:ultrastructure; D018532:Pisum sativum / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects* / Q000648:ultrastructure; D018517:Plant Roots / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects / Q000648:ultrastructure; D010947:Plants, Toxic; D014026:Nicotiana / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects* / Q000648:ultrastructure
chemical_list: D003037:Cobalt Radioisotopes; D007477:Ions; D006371:Helium; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate