The chemokine interleukin-8 acutely reduces Ca(2+) currents in identified cholinergic septal neurons expressing CXCR1 and CXCR2 receptor mRNAs.
The chemokine IL-8 is known to be synthesized by glial cells in the brain. It has traditionally been shown to have an important role in neuroinflammation but recent evidence indicates that it may also be involved in rapid signaling in neurons. We investigated how IL-8 participates in rapid neuronal signaling by using a combination of whole-cell recording and single-cell RT-PCR on dissociated rat septal neurons. We show that IL-8 can acutely reduce Ca(2+) currents in septal neurons, an effect that was concentration-dependent, involved the closure of L- and N-type Ca(2+) channels, and the activation of G(ialpha1) and/or G(ialpha2) subtype(s) of G-proteins. Analysis of the mRNAs from the recorded neurons revealed that the latter were all cholinergic in nature. Moreover, we found that all cholinergic neurons that responded to IL-8, expressed mRNAs for either one or both IL-8 receptors CXCR1 and CXCR2. This is the first report of a chemokine that modulates ion channels in neurons via G-proteins, and the first demonstration that mRNAs for CXCR1 are expressed in the brain. Our results suggest that IL-8 release by glial cells in vivo may activate CXCR1 and CXCR2 receptors on cholinergic septal neurons and acutely modulate their excitability by closing calcium channels.
Puma
C
C
McGill University, Department of Psychiatry, Douglas Hospital Research Center, Verdun, Quebec, Canada.
Danik
M
M
Quirion
R
R
Ramon
F
F
Williams
S
S
D000109:Acetylcholine / Q000502:physiology; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002121:Calcium Channel Blockers / Q000494:pharmacology; D020746:Calcium Channels, L-Type / Q000378:metabolism; D020864:Calcium Channels, N-Type / Q000378:metabolism; D002450:Cell Communication / Q000502:physiology; D002799:Cholinergic Fibers / Q000187:drug effects / Q000502:physiology; D019204:GTP-Binding Proteins / Q000378:metabolism; D015870:Gene Expression / Q000276:immunology; D016244:Guanosine 5'-O-(3-Thiotriphosphate) / Q000494:pharmacology; D016209:Interleukin-8 / Q000494:pharmacology*; D009457:Neuroglia / Q000166:cytology / Q000502:physiology; D009474:Neurons / Q000187:drug effects* / Q000502:physiology* / Q000648:ultrastructure; D009924:Organ Culture Techniques; D018408:Patch-Clamp Techniques; D011188:Potassium / Q000494:pharmacology; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D023062:Receptors, Interleukin-8A / Q000235:genetics*; D023063:Receptors, Interleukin-8B / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020665:Septum of Brain / Q000166:cytology; D013268:Stimulation, Chemical; D020866:omega-Conotoxin GVIA / Q000494:pharmacology
D002121:Calcium Channel Blockers; D020746:Calcium Channels, L-Type; D020864:Calcium Channels, N-Type; D016209:Interleukin-8; D012333:RNA, Messenger; D023062:Receptors, Interleukin-8A; D023063:Receptors, Interleukin-8B; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D020866:omega-Conotoxin GVIA; D019204:GTP-Binding Proteins; D000109:Acetylcholine; D011188:Potassium; D002118:Calcium