title: Low glucosinolate Brassica juncea breeding line revealed to be nullisomic.
abstract: The low glucosinolate Brassica juncea breeding line 1058 was derived from a BC1F3 plant of an interspecific cross between high glucosinolate Indian B. juncea (genome AABB, 2n = 36) line 60143 and B. rapa (genome AA, 2n = 20) canola strain CZY. Line 60143 had 2n = 36 chromosomes (18 bivalents at metaphase I) and strain CZY had 2n = 20 chromosomes (10 bivalents). Line 1058 was nullisomic, with 2n - 2 = 34 chromosomes, with 17 bivalents formed at metaphase I and an even chromosomal segregation of 17:17 at anaphase I. In F1 hybrid plants of the cross 1058 x CZY, 98.3% of the pollen mother cells had 10 bivalents and seven univalents. This is evidence that plants of line 1058 are nullisomic, missing one pair of B-genome chromosomes.
authors.affiliation: Agriculture and Agri-Food Canada, Saskatoon Research Centre, Saskatoon, SK, Canada.
authors.forename: B F
authors.initials: BF
authors.lastname: Cheng
authors.forename: G
authors.initials: G
authors.lastname: Séguin-Swartz
authors.forename: D J
authors.initials: DJ
authors.lastname: Somers
authors.forename: G
authors.initials: G
authors.lastname: Rakow
mesh_terms: D001937:Brassica / Q000235:genetics*; D002678:Chimera; D003433:Crosses, Genetic; D005961:Glucosinolates / Q000378:metabolism; D009693:Nucleic Acid Hybridization
chemical_list: D005961:Glucosinolates