title: Cytotoxic evaluation of root canal filling materials on primary human oral fibroblast cultures and a permanent hamster cell line.
abstract: To date there has been very little data on the cytotoxic response of different cell lines to root canal sealers. The objective of this study was to determine the cytocompatibility of three different extracts of root canal sealers and to compare the cytotoxic response of these materials on two different primary human oral fibroblasts (derived from gingiva and buccal mucosa) and one permanent hamster cell line (V79 cells). Cytotoxicity was judged using an assay of tetrazolium bromide reduction. The results showed that root canal sealers (AH plus, Canals, and N2) were cytotoxic to primary human oral fibroblast cultures and V79 cells. It was found that N2 was the most toxic root canal sealer among those tested in all cultures. The toxicity decreased in an order of N2 > AH plus approximately = Canals. The sensitivity of toxicity depended on the materials tested and the cell culture system used.
authors.affiliation: Department of Dentistry, Chung Shan Medical and Dental College Hospital, Taichung, Taiwan.
authors.forename: K W
authors.initials: KW
authors.lastname: Tai
authors.forename: F M
authors.initials: FM
authors.lastname: Huang
authors.forename: Y C
authors.initials: YC
authors.lastname: Chang
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D002460:Cell Line / Q000187:drug effects; D003124:Colorimetry; D004396:Coloring Agents; D006224:Cricetinae; D003412:Cricetulus; D004338:Drug Combinations; D004853:Epoxy Resins / Q000633:toxicity; D005054:Eugenol / Q000633:toxicity; D005347:Fibroblasts / Q000187:drug effects*; D005557:Formaldehyde / Q000633:toxicity; D005881:Gingiva / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D009061:Mouth Mucosa / Q000166:cytology / Q000187:drug effects*; D012387:Root Canal Filling Materials / Q000633:toxicity*; D013778:Tetrazolium Salts; D013844:Thiazoles; D015034:Zinc Oxide / Q000633:toxicity; D015035:Zinc Oxide-Eugenol Cement / Q000633:toxicity
chemical_list: D004396:Coloring Agents; D004338:Drug Combinations; D004853:Epoxy Resins; D012387:Root Canal Filling Materials; D013778:Tetrazolium Salts; D013844:Thiazoles; D015035:Zinc Oxide-Eugenol Cement; C112894:canals sealer; C534916:epoxy resin-based root canal sealer; D005557:Formaldehyde; D005054:Eugenol; C003799:N2 Dental Cement; C022616:thiazolyl blue; D015034:Zinc Oxide