Effect of lindane on antioxidant enzymes in epididymis and epididymal sperm of adult rats.
OBJECTIVE
To find out the changes induced by lindane on the antioxidant enzymes in epididymis and epididymal sperm of adult rats.


METHODS
Adult male rats were orally administered lindane at a dose of 5.0 mg/kg body weight per day for 30 days. At the end of the treatment, the rats were sacrificed. The epididymis was removed and weighed and sperm were collected for sperm count, motility and biochemical studies. A 1% homogenate of epididymis was prepared and used for biochemical estimations.


RESULTS
In lindane-treated rats, there were significant reductions in the epididymal weight, epididymal sperm count and motility compared with the controls. Significant decreases in the superoxide dismutase (SOD), catalase, glutathione reductase and glutathione peroxidase activities and significant increases in the H2O2 generation and lipid peroxidation were also observed in the epididymis and epididymal sperm of lindane-treated rats.


CONCLUSIONS
Lindane decreases the levels of antioxidant enzymes in the epididymis and epididymal sperm of adult rats thereby inducing oxidative stress.
Chitra
K C
KC
School of Life Sciences, Pondicherry University, India.
Sujatha
R
R
Latchoumycandane
C
C
Mathur
P P
PP
D000367:Age Factors; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D004822:Epididymis / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D001556:Hexachlorocyclohexane / Q000378:metabolism* / Q000494:pharmacology*; D008297:Male; D009929:Organ Size; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D013094:Spermatozoa / Q000187:drug effects* / Q000201:enzymology; D013482:Superoxide Dismutase / Q000378:metabolism
D000975:Antioxidants; D017382:Reactive Oxygen Species; D001556:Hexachlorocyclohexane; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase