Voltammetric determination of praziquantel in tablets and biological fluids.
A simple method is described for the determination of praziquantel (CAS 55268-74-1) in its pure form, tablet formulations and biological fluids. The proposed method depends upon the polarographic activity of praziquantel at the dropping mercury electrode (DME) in Britton Robinson buffers, whereby a well-defined catholic wave is produced over the pH range 7-12. The wave was characterized as being irreversible diffusion-controlled with limited adsorption properties. The diffusion current constant (Id) was 0.56 +/- 0.004 (n = 11). The current-concentration relationship was found to be rectilinear over the range 8-48, 3.2-38.4 and 0.48-20 micrograms.ml-1 using direct current (DCt), differential pulse polarographic (DPP) and alternating current (ACt) odes, respectively, with minimum detection limit (S/N = 2) of 0.32 microgram.ml-1 (1.02 x 10(-6) mol/l and 0.02 microgram.ml-1 (6.4 x 10(-8) mol/l) for DPP and ACt modes respectively. The average percent recovery was favourably compared to a reference method with a satisfactory standard deviation. The proposed method was applied to spiked human urine and plasma. The percentage recoveries were 99.33 +/- 0.79 and 98.23 +/- 0.53, respectively.
Rizk
M
M
Department of Analytical Chemistry, Faculty of Pharmacy, University of Mansoura, Mansoura, Egypt. phrfac@mum.mans.eun.eg
Belal
F
F
Ibrahim
F
F
Ahmed
S
S
el-Enany
N M
NM
D000980:Antiplatyhelmintic Agents / Q000032:analysis* / Q000097:blood / Q000652:urine; D002138:Calibration; D004563:Electrochemistry; D004566:Electrodes; D006801:Humans; D006863:Hydrogen-Ion Concentration; D011048:Polarography; D011223:Praziquantel / Q000032:analysis* / Q000097:blood / Q000652:urine; D013607:Tablets
D000980:Antiplatyhelmintic Agents; D013607:Tablets; D011223:Praziquantel