Heteroduplex mobility assay of the D1/D2 region of the 26S rDNA for differentiation of Saccharomyces species.
OBJECTIVE
We present the HMA method for Saccharomyces differentiation using the PCR amplified D1/D2 26S rDNA.


RESULTS
This methodology is based on heteroduplex formation when two different DNAs are hybridized. We tested 11 type cultures of Saccharomyces, 27 different cultures of S. cerevisiae and four other ascomycetic genera.


CONCLUSIONS
The method was capable of differentiating Saccharomyces species and was mainly very efficient for S. cerevisiae identification. HMA can probably be applied in other genera, where identification is sometimes difficult only by conventional traits, which are based on physiology and morphology.


CONCLUSIONS
HMA provides a rapid and relatively simple molecular tool, contributing for yeast taxonomy.
Ramos
J P
JP
Departamento de Genética, Instituto de Biologia, CCS, bloco A, Universidade Federal do Rio de Janeiro, Ilha do Fundão, Rio de Janeiro, CEP: 21944-970, Brazil. jpramos@acd.ufrj.br
Valente
P
P
de Souza
R A
RA
Rosa
C A
CA
Leoncini
O
O
D004271:DNA, Fungal / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D020180:Heteroduplex Analysis; D016533:Mycological Typing Techniques; D009692:Nucleic Acid Heteroduplexes; D016133:Polymerase Chain Reaction; D012335:RNA, Ribosomal / Q000235:genetics; D012440:Saccharomyces / Q000145:classification* / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000145:classification / Q000235:genetics; D012680:Sensitivity and Specificity
D004271:DNA, Fungal; D004275:DNA, Ribosomal; D009692:Nucleic Acid Heteroduplexes; D012335:RNA, Ribosomal; C065096:RNA, ribosomal, 26S