Formation of cis-coniferin in cell-free extracts of Fagus grandifolia Ehrh bark.
American beech (Fagus grandifolia Ehrh) bark exclusively accumulates cis-monolignols and their glucosidic conjugates; no evidence for the accumulation of trans-monolignols has been found.  The glucosyltransferase from this source exhibits a very unusual substrate specificity for cis, and not trans, monolignols.  This is  further evidence that cis monolignols are involved in lignin formation in these plant tissues.  Preliminary evidence for the existence of a novel trans-cis monolignol isomerase was obtained, in agreement with our contention that this isomerization is not photochemically mediated.
Yamamoto
E
E
Departments of Wood Science and Forest Products, and Biochemistry, Virginia Polytechnic Institute and State University, Blacksburg 24061.
Inciong
E J
EJ
Davin
L B
LB
Lewis
N G
NG
D002473:Cell Wall / Q000201:enzymology; D002934:Cinnamates / Q000737:chemistry; D046508:Culture Techniques; D005960:Glucosides / Q000737:chemistry / Q000378:metabolism; D005964:Glucosyltransferases / Q000737:chemistry / Q000378:metabolism*; D007535:Isomerases / Q000737:chemistry / Q000378:metabolism; D008031:Lignin / Q000096:biosynthesis* / Q000378:metabolism; D010636:Phenols / Q000737:chemistry / Q000378:metabolism; D010936:Plant Extracts / Q000378:metabolism; D013379:Substrate Specificity; D014197:Trees / Q000201:enzymology* / Q000378:metabolism
D002934:Cinnamates; D005960:Glucosides; D010636:Phenols; D010936:Plant Extracts; D008031:Lignin; C010559:coniferyl alcohol; D005964:Glucosyltransferases; D007535:Isomerases; C016316:coniferin