Molecular strain typing of Campylobacter jejuni by pulsed-field gel electrophoresis in a single day.
Rapid molecular strain typing is critical for effective outbreak investigation and implementation of infection control measures. Pulsed-field gel electrophoresis is a highly discriminatory technique for Campylobacter jejuni, but generally requires 3-5 days. We describe a simplified protocol for pulsed-field gel electrophoresis that provides high quality typing of C. jejuni isolates in a single day.
Michaud
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S
VA Medical Center, and Department of Medicine Boston University School of Medicine, MA 02130, USA. smichaud@courrier.usherb.ca
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D000328:Adult; D000368:Aged; D015373:Bacterial Typing Techniques; D002169:Campylobacter Infections / Q000453:epidemiology* / Q000382:microbiology; D016123:Campylobacter jejuni / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000032:analysis*; D016521:Electrophoresis, Gel, Pulsed-Field; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D017720:Molecular Epidemiology; D013997:Time Factors
D004269:DNA, Bacterial