Incorporation of positively charged deoxynucleic S-methylthiourea linkages into oligodeoxyribonucleotides.
Oligodeoxyribonucleic acids (15- and 18-mers) containing both negatively charged phosphate and positively charged S-methyl thiourea internucleoside linkages (DNmt/DNA chimera) have been synthesized. DNA binding characteristics and nuclease resistance of DNmt/DNA chimera have been evaluated.
Challa
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Department of Chemistry and Biochemistry, University of California, Santa Barbara, 93106, USA.
Bruice
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D020137:Base Pair Mismatch; D001665:Binding Sites; D001671:Biochemistry / Q000379:methods*; D002942:Circular Dichroism; D005090:Exodeoxyribonucleases / Q000378:metabolism; D017403:In Situ Hybridization / Q000379:methods; D009838:Oligodeoxyribonucleotides / Q000737:chemistry* / Q000378:metabolism; D013890:Thiourea / Q000737:chemistry
D009838:Oligodeoxyribonucleotides; C120262:methylthioisourea; D005090:Exodeoxyribonucleases; C028450:exodeoxyribonuclease I; D013890:Thiourea