title: Derivatization and template-guided ligation of oligodeoxyribonucleotides using cyanogen bromide and N-substituted morpholines.
abstract: Cyanogen bromide has been found to induce the template-guided condensation of oligonucleotides only in the presence of N-substituted morpholines. Based on 31P, 1H and 13C NMR spectroscopy data, the mechanism of the phosphomonoester group activation by cyanogen bromide in N-substituted morpholine buffers is suggested. It has also been shown that BrCN can be used for the synthesis of oligonucleotide derivatives in aqueous solution.
authors.affiliation: Chemistry Department, Lomonosov Moscow State University, Russia.
authors.forename: Z A
authors.initials: ZA
authors.lastname: Shabarova
authors.forename: O A
authors.initials: OA
authors.lastname: Fedorova
authors.forename: N G
authors.initials: NG
authors.lastname: Dolinnaya
authors.forename: M B
authors.initials: MB
authors.lastname: Gottikh
mesh_terms: D002021:Buffers; D003488:Cyanogen Bromide / Q000737:chemistry*; D004247:DNA / Q000737:chemistry; D019143:Evolution, Molecular; D009025:Morpholines / Q000737:chemistry*; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D009841:Oligonucleotides / Q000138:chemical synthesis*; D013698:Templates, Genetic
chemical_list: D002021:Buffers; D009025:Morpholines; D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; D004247:DNA; D003488:Cyanogen Bromide