Strand displacement recognition of mixed adenine-cytosine sequences in double stranded DNA by thymine-guanine PNA (peptide nucleic acid).
Mixed pyrimidine-purine peptide nucleic acids (PNAs) composed of thymines and guanines are shown to form a PNA(2)-DNA triplex with Watson-Crick complementary adenine-cytosine oligonucleotides and to bind complementary adenine-cytosine targets in double stranded DNA by helix invasion. These results for the first time demonstrate binding of an unmodified PNA oligomer to a mixed pyrimidine-purine target in double stranded DNA and illustrate a novel binding mode of PNA.
Nielsen
P E
PE
Center for Biomolecular Recognition, Department for Biochemistry and Genetics, Biochemistry Laboratory B, The Panum Institute, Blegdamsvej 3c, DK-2200N Copenhagen, Denmark. pen@imbg.ku.dk
Egholm
M
M
D001482:Base Composition; D020029:Base Pairing; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D015226:Dinucleoside Phosphates / Q000378:metabolism*; D006147:Guanine; D006860:Hydrogen Bonding; D009691:Nucleic Acid Denaturation; D020135:Peptide Nucleic Acids / Q000737:chemistry / Q000378:metabolism*; D013696:Temperature; D013941:Thymine
D015226:Dinucleoside Phosphates; D020135:Peptide Nucleic Acids; C051802:triplex DNA; C013797:adenylyl cytidine; D006147:Guanine; D004247:DNA; D013941:Thymine