Requirement of tissue-selective TBP-associated factor TAFII105 in ovarian development.
Transcription factor TFIID, composed of TBP and TAFII subunits, is a central component of the RNA polymerase II machinery. Here, we report that the tissue-selective TAFII105 subunit of TFIID is essential for proper development and function of the mouse ovary. Female mice lacking TAFII105 are viable but infertile because of a defect in folliculogenesis correlating with restricted expression of TAFII105 in the granulosa cells of the ovarian follicle. Gene expression profiling has uncovered a defective inhibin-activin signaling pathway in TAFII105-deficient ovaries. Together, these studies suggest that TAFII105 mediates the transcription of a subset of genes required for proper folliculogenesis in the ovary and establishes TAFII105 as a cell type-specific component of the mammalian transcriptional machinery.
Freiman
R N
RN
Department of Molecular and Cell Biology, Howard Hughes Medical Institute, University of California at Berkeley, Berkeley, CA 94720-3204, USA.
Albright
S R
SR
Zheng
S
S
Sha
W C
WC
Hammer
R E
RE
Tjian
R
R
D000818:Animals; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D015536:Down-Regulation; D005260:Female; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D006107:Granulosa Cells / Q000378:metabolism / Q000502:physiology*; D017403:In Situ Hybridization; D007247:Infertility, Female; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D020411:Oligonucleotide Array Sequence Analysis; D009929:Organ Size; D009928:Organ Specificity; D006080:Ovarian Follicle / Q000254:growth & development*; D010053:Ovary / Q000166:cytology / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D010060:Ovulation; D021122:Protein Subunits; D015398:Signal Transduction; D035381:TATA-Binding Protein Associated Factors; D035362:Transcription Factor TFIID; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D019473:Transcription Factors, TFII / Q000378:metabolism; D014158:Transcription, Genetic
D004268:DNA-Binding Proteins; D021122:Protein Subunits; D035381:TATA-Binding Protein Associated Factors; C491103:Taf4b protein, mouse; D035362:Transcription Factor TFIID; D014157:Transcription Factors; D019473:Transcription Factors, TFII