Molecular cloning and sequence of Sparus aurata skeletal myosin light chains expressed in white muscle: developmental expression and thyroid regulation.
Two full-length cDNA clones encoding the skeletal myosin light chain 2 (MLC2; 1452bp) and myosin light chain 3 (MLC3; 972bp) were isolated from a cDNA library prepared from gilthead sea bream Sparus aurata larvae. The MLC2 cDNA encoded a predicted protein of 170 residues that was 79% identical to rabbit MLC2 over the entire length and 87% identical within the Ca(2+)-binding region. The deduced amino acid sequence of MLC3 was 153 residues in length and was 91% and 69% identical to the zebrafish and rabbit MLC3, respectively. Northern blot analysis revealed that in adults both transcripts were expressed in fast white muscle only. MLC2 appeared earlier in development: MLC2 transcripts were detectable from the beginning of segmentation, whereas MLC3 transcripts did not appear until 27h post-fertilisation. At this developmental stage, a second MLC2 transcript of 0.89 kilobase-pairs was present. MLCs exhibited a different age-related pattern of response to varied thyroidal states, which were experimentally induced by the administration of 1 microg g(-1)body mass of thyroxine (T4) or triiodothyronine (T3), or 5 ng g(-1)body mass of the hypothyroidal compound thiourea; MLC3 expression was not significantly affected, whereas levels of MLC2 transcripts were significantly elevated in the white muscle only of juvenile sea bream after administration of T4. Although the mechanism of thyroidal regulation of MLC expression remains unknown, the present results suggest that different regulatory mechanisms exist for different MLCs.
Moutou
K A
KA
Department of Biochemistry and Biotechnology, University of Thessaly, 26 Ploutonos Street, 41221 Larissa, Greece. kmoutou@uth.gr
Canario
A V
AV
Mamuris
Z
Z
Power
D M
DM
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000302:isolation & purification; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects*; D015723:Gene Library; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000737:chemistry*; D018994:Myosin Light Chains / Q000737:chemistry / Q000235:genetics*; D010473:Perciformes / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013963:Thyroid Hormones / Q000494:pharmacology*; D013974:Thyroxine / Q000097:blood / Q000494:pharmacology; D014284:Triiodothyronine / Q000097:blood / Q000494:pharmacology
D018076:DNA, Complementary; D018994:Myosin Light Chains; D013963:Thyroid Hormones; D014284:Triiodothyronine; D013974:Thyroxine