Antisense oligonucleotide ISIS 2922 targets IE-expression and prevents HCMV-IE-induced suppression of TSP-1 and TSP-2 expression.
ISIS 2922, but not ganciclovir (GCV), inhibits HCMV immediate early protein (IE) expression in different infected cell lines and prevents down-modulation of extracellular matrix proteins thrombospondin-1 and -2 induced by IE proteins. While action of ISIS 2922 is mainly due to specific inhibition of IE 2 mRNA, there is also evidence for unspecific effects in terms of inhibition of virus adhesion and penetration.
Margraf
S
S
J. W. Goethe University Hospital, Inst. f. Med. Virology, Paul Ehrlich-Str. 40, D-60596 Frankfurt am Main, Germany.
Bittoova
M
M
Vogel
J U
JU
Kotchekov
R
R
Doerr
H W
HW
Cinatl
J
J
D000956:Antigens, Viral / Q000096:biosynthesis*; D000998:Antiviral Agents / Q000494:pharmacology*; D006801:Humans; D017874:Immediate-Early Proteins / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D013873:Thionucleotides / Q000494:pharmacology*; D019700:Thrombospondin 1 / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D019699:Thrombospondins / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D000099262:Thrombospondin 2
D000956:Antigens, Viral; D000998:Antiviral Agents; D017874:Immediate-Early Proteins; D016376:Oligonucleotides, Antisense; D013873:Thionucleotides; D019700:Thrombospondin 1; D019699:Thrombospondins; D000099262:Thrombospondin 2; C044582:immediate-early proteins, cytomegalovirus; C091346:fomivirsen