Obtaining force-frequency curves with a single 3-second train of stimuli.
We devised a method to assess the force-frequency relationship (FFR) in human skeletal muscle that involved delivery of a single 2.8-s train of shocks directly to the femoral nerve. This increasing-frequency train (IFT) was based on a power function, with a range of stimulation frequencies beginning at 5 Hz and rising to 100 Hz. We compared the IFT to a standard series of constant-frequency trains (CFT) under two conditions. Force-frequency curves were examined, first in response to altered muscle length and second, following fatigue. There was no leftward shift in the curve when the knee extensors were shortened, although maximal force increased. In contrast, we observed a rightward shift in the curve after fatigue with both protocols; the frequency required to develop 50% of maximal force increased by 48% (P <.01) with CFT and 58% (P <.001) with an IFT. The CFT produced an irregular pattern of low-frequency fatigue recovery. In the IFT, low-frequency fatigue was greatest at the onset of recovery and decreased linearly until 120 s. These experiments show that the IFT protocol reveals alterations in muscle performance similar to the more traditional CFT. However, it requires only 2.8 s to administer and was judged more tolerable by 70% of our subjects. This suggests that the IFT may be an effective alternative for determining the FFR in human muscle for clinical and experimental purposes.
Meyers
B M
BM
Department of Kinesiology and Health Science, 346 Bethune College, York University, 4700 Keele Street, Toronto, Ontario M3J 1P3, Canada.
Nguyen
J
J
Cafarelli
E
E
D000328:Adult; D004558:Electric Stimulation; D004576:Electromyography / Q000379:methods*; D005260:Female; D006801:Humans; D008297:Male; D009119:Muscle Contraction / Q000502:physiology; D018763:Muscle Fatigue / Q000502:physiology; D018482:Muscle, Skeletal / Q000502:physiology*