Inactivation of S-adenosyl-L-homocysteine hydrolase with fluorinated analogs of 2'- and 3'-deoxy-5'-methylthioadenosine.
Fluorinated analogs of 2'- and 3'-deoxy-5'-methylthioadenosine 1-4 caused irreversible inactivation of AdoHcy hydrolase. Based on the ESI-Mass spectra analysis of the inactivated enzyme with the fluorinated analog 1 a mechanism of inactivation is proposed.
Guillerm
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Laboratoire des Réactions Sélectives et Applications-UMR 6519, Université de Reims, BP 1039, 51687 Reims, France.
Guillerm
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Witkowski-Vandenplas
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D043383:Adenosylhomocysteinase; D003839:Deoxyadenosines / Q000138:chemical synthesis / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000138:chemical synthesis / Q000494:pharmacology*; D006801:Humans; D006845:Hydrocarbons, Fluorinated / Q000138:chemical synthesis / Q000494:pharmacology*; D006867:Hydrolases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D010920:Placenta / Q000201:enzymology; D011994:Recombinant Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism; D013872:Thionucleosides / Q000138:chemical synthesis / Q000494:pharmacology*
D003839:Deoxyadenosines; D004791:Enzyme Inhibitors; D006845:Hydrocarbons, Fluorinated; D011994:Recombinant Proteins; D013872:Thionucleosides; D006867:Hydrolases; D043383:Adenosylhomocysteinase