Efficient synthesis of D-[1'-13C]-ribonucleosides for incorporation into oligo-RNA.
Syntheses of the monomer building blocks used for the solid-phase synthesis of specifically 1'-13C-labeled oligoribonucleotides from the D-[1-13C]ribose is presented. Procedure has been used for the selective formation of 2'-O-silylated ribonucleosides. After 5'-O-dimethoxytritylation, the synthesis of D-[1'-13C] ribonucleoside phosphoramidites has been achieved.
Saito
Y
Y
I.C.O.A. associé CNRS, Faculté des Sciences, 45100 Orléans-France.
Nyilas
A
A
Agrofoglio
L A
LA
D002247:Carbon Isotopes; D007553:Isotope Labeling / Q000379:methods*; D009943:Organophosphorus Compounds / Q000138:chemical synthesis; D012313:RNA / Q000138:chemical synthesis*; D012263:Ribonucleosides / Q000138:chemical synthesis*
D002247:Carbon Isotopes; D009943:Organophosphorus Compounds; D012263:Ribonucleosides; C434331:phosphoramidite; D012313:RNA