title: Sublethal effects of ultraviolet A radiation on Enterobacter cloacae.
abstract: We report the sublethal effects of ultraviolet A (UVA) on Enterobacter cloacae in comparison with those produced in Escherichia coli. UVA-induced sublethal effects were investigated in either bacterial membrane and at tRNA level. Limited dependence on oxygen concentration for photoinduced inhibition of biochemical membrane functions and low levels of oxidative damage during the irradiation period were found in En. cloacae. On the other hand, ultraviolet spectroscopy and reversed-phase HPLC analysis of hydrolysed tRNA showed that radio induced damage to tRNA is similar in En. cloacae and E. coli. Nevertheless, growth delay induced by UVA in En. cloacae was shorter than that found in E. coli submitted to the same experimental conditions. A limited post-irradiation ppGpp accumulation and the absence of any influence of the membrane damage on the growth delay extent seem to be responsible for the shortness of this effect in En. cloacae. Most of the differences between En. cloacae and E. coli could be attributed to an increased ability of En. cloacae to overcome oxidative stress during UVA exposure.
authors.affiliation: Departamento de Radiobiología, Comisión Nacional de Energía Atómica, Av. General Paz 1499, 1650, General San Martín, Buenos Aires, Argentina. oppezzo@cnea.gov.ar
authors.forename: O J
authors.initials: OJ
authors.lastname: Oppezzo
authors.forename: R A
authors.initials: RA
authors.lastname: Pizarro
mesh_terms: D002462:Cell Membrane / Q000528:radiation effects; D003432:Cross-Linking Reagents; D016972:Enterobacter cloacae / Q000235:genetics / Q000254:growth & development / Q000528:radiation effects*; D012329:RNA, Bacterial / Q000378:metabolism; D012360:RNA, Transfer, Phe / Q000378:metabolism; D012361:RNA, Transfer, Pro / Q000378:metabolism; D014466:Ultraviolet Rays
chemical_list: D003432:Cross-Linking Reagents; D012329:RNA, Bacterial; D012360:RNA, Transfer, Phe; D012361:RNA, Transfer, Pro