Activation and localization of cartilage-derived morphogenetic protein-1 at the site of ossification of the ligamentum flavum.
Localization and expression of cartilage-derived morphogenetic protein (CDMP)-1 in tissues at the site of ossification of the ligamentum flavum (OLF) were examined by immunohistochemistry and in situ hybridization. The CDMP-1 protein and messenger ribonucleic acid (mRNA) were localized in spindle-shaped cells and chondrocytes in the OLF tissues. CDMP-1 was not detected in cells in non-ossified sites. These data indicate that CDMP-1 is locally activated and localized in spindle-shaped cells and chondrocytes at the site of OLE. Given the previously reported promoting action of CDMP-1 for chondrogenesis, the current results suggest that CDMP-1 may be involved in the progression of OLF, leading to the narrowing of spinal canal and thus causing severe clinical manifestations.
Nakase
T
T
Department of Orthopaedic Surgery, Osaka University Medical School, Suita, Japan. nakase@ort.med.osaka-u.ac.jp
Ariga
K
K
Yonenobu
K
K
Tsumaki
N
N
Luyten
F P
FP
Mukai
Y
Y
Sato
I
I
Yoshikawa
H
H
D000368:Aged; D019485:Bone Morphogenetic Proteins; D019902:Chondrocytes / Q000378:metabolism; D055428:Growth Differentiation Factor 5; D006133:Growth Substances / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D017843:Ligamentum Flavum / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009999:Ossification, Heterotopic / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D016212:Transforming Growth Factor beta / Q000378:metabolism*
D019485:Bone Morphogenetic Proteins; D055428:Growth Differentiation Factor 5; D006133:Growth Substances; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta