Protein kinase C and changes in manganese superoxide dismutase gene expression in cultured glial cells.
1. To study the role of protein kinase C (PKC) in the increase in manganese superoxide dismutase (Mn-SOD) gene expression following transient hypoxia in glial cells, we examined the mRNA levels of Mn-SOD using northern blot analysis. 2. The Mn-SOD mRNA levels were markedly increased after exposure to nitrogen gas for 5 min. 3. Pretreatment with chelerythrine or GF109203x, inhibitors of PKC, attenuated the increase in Mn-SOD mRNA following hypoxia in a concentration-dependent manner. 4. Incubation with phorbol 12-myristate 13-acetate, the PKC activator, enhanced the increase in Mn-SOD gene expression in response to transient hypoxia. 5. The results suggest that hypoxia increases Mn-SOD gene expression in cultured glial cells mainly through activation of a PKC pathway.
Huang
W C
WC
Department of Pharmacology, College of Medicine, National Cheng Kung University, Tainan City, Taiwan.
Chen
P C
PC
Jou
S B
SB
Cheng
J T
JT
D015687:Cell Hypoxia / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005786:Gene Expression Regulation; D009457:Neuroglia / Q000187:drug effects / Q000201:enzymology*; D011493:Protein Kinase C / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000096:biosynthesis; D013482:Superoxide Dismutase / Q000096:biosynthesis* / Q000235:genetics*
D004791:Enzyme Inhibitors; D012333:RNA, Messenger; D013482:Superoxide Dismutase; D011493:Protein Kinase C