Crystallisation of alpha-crustacyanin, the lobster carapace astaxanthin-protein: results from EURECA.
Crystallisation of alpha-crustacyanin, the lobster carapace astaxanthin-protein was attempted under microgravity conditions in EURECA satellite using liquid-liquid diffusion with polyethyleneglycol (PEG) as precipitant; in a second reaction chamber phenol and dioxan were used as additives to prevent composite crystal growth. Crystals of alpha-crustacyanin grown under microgravity from PEG were larger than those grown terrestrially in the same apparatus under otherwise identical conditions. On retrieval, the crystals from PEG were shown to be composite and gave a powder diffraction pattern. The second reaction chamber showed leakage on retrieval and had also been subjected to rapid temperature variation during flight. Crystal fragments were nevertheless recovered but showed a powder diffraction pattern. It is concluded, certainly for liquid-liquid diffusion using PEG alone, that, for crustacyanin, although microgravity conditions resulted in an increase in dimensions of crystals, a measurable improvement in molecular ordering was not achieved.
Zagalsky
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PF
Biochemistry Department, University of London, Egham, UK.
Wright
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CE
Parsons
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M
D000818:Animals; D019149:Bioreactors; D002352:Carrier Proteins; D003460:Crystallization; D004058:Diffusion; D008854:Microscopy, Electron; D015394:Molecular Structure; D008121:Nephropidae; D011092:Polyethylene Glycols; D011506:Proteins / Q000737:chemistry*; D013026:Space Flight; D014893:Weightlessness
D002352:Carrier Proteins; D011506:Proteins; C018494:crustacyanins; D011092:Polyethylene Glycols