DNA triple-helix formation at pyrimidine-purine inversion sites.
A systematic investigation of a series of triplex forming oligonucleotides (TFOs) containing alpha-, and beta-thymidine, alpha- and beta-N7-hypoxanthine, and alpha- and beta-N7 and N9 aminopurine nucleosides, designed to bind to T-A inversion sites in DNA target sequences was performed. Data obtained from gel mobility assays indicate that T-A recognition in the antiparallel triple-helical binding motif is possible if the nucleoside alpha N9-aminopurine is used opposite to the inversion site in the TFO.
Parel
S P
SP
Department of Chemistry and Biochemistry, University of Bern, CH-3012 Bern, Switzerland.
Marfurt
J
J
Leumann
C J
CJ
D000225:Adenine / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D019271:Hypoxanthine / Q000737:chemistry; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000737:chemistry; D013936:Thymidine / Q000737:chemistry*
D009841:Oligonucleotides; D019271:Hypoxanthine; D004247:DNA; D000225:Adenine; D013936:Thymidine