title: Substrate specificity of the streptococcal cysteine protease.
abstract: The streptococcal pyrogenic exotoxin B (SpeB) is an important factor in mediating Streptococcus pyogenes infections. SpeB is the zymogen of the streptococcal cysteine protease (SCP), of which relatively little is known regarding substrate specificity. To investigate this aspect of SCP function, a series of internally quenched fluorescent substrates was designed based on the cleavage sites identified in the autocatalytic processing of SpeB to mature SCP. The best substrates for SCP contain three amino acids in the nonprimed position (i.e. AIK in P(3)-P(2)-P(1)). Varying the length of the substrate on the primed side of the scissile bond has a relatively lower effect on activity. The highest activity (k(cat)/K(M) = 2.8 +/- 0.6 (10(5) x m(-1)s(-1)) is observed for the pentamer 3-aminobenzoic acid-AIKAG-3-nitrotyrosine, which spans subsites S(3) to S(2)' on the enzyme. High pressure liquid chromatography and mass spectrometry analyses show that the substrates are cleaved at the site predicted from the autoprocessing experiments. These results show that SCP can display an important level of endopeptidase activity. Substitutions at position P(2) of the substrate clearly indicate that the S(2) subsite of SCP can readily accommodate substrates containing a hydrophobic residue at that position and that some topological preference exists for that subsite. Substitutions in positions P(3), P(1), and P(1)' had little or no effect on SCP activity. The substrate specificity outlined in this work further supports the similarity between SCP and the cysteine proteases of the papain family. From the data regarding the identified or proposed natural substrates for SCP, it appears that this substrate specificity profile may also apply to the processing of mammalian and streptococcal protein targets by SCP.
authors.affiliation: Biotechnology Research Institute, National Research Council of Canada, Montreal, Quebec H4P 2R2, Canada.
authors.forename: M
authors.initials: M
authors.lastname: Nomizu
authors.forename: G
authors.initials: G
authors.lastname: Pietrzynski
authors.forename: T
authors.initials: T
authors.lastname: Kato
authors.forename: P
authors.initials: P
authors.lastname: Lachance
authors.forename: R
authors.initials: R
authors.lastname: Menard
authors.forename: E
authors.initials: E
authors.lastname: Ziomek
mesh_terms: D001665:Binding Sites; D002384:Catalysis; D002851:Chromatography, High Pressure Liquid; D003546:Cysteine Endopeptidases / Q000737:chemistry* / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007700:Kinetics; D013058:Mass Spectrometry; D009154:Mutation; D013297:Streptococcus pyogenes / Q000201:enzymology*; D013379:Substrate Specificity; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000494:pharmacology
chemical_list: C002744:3-nitrotyrosine; D014443:Tyrosine; D003546:Cysteine Endopeptidases