title: Methanohalophilus zhilinae sp. nov., an alkaliphilic, halophilic, methylotrophic methanogen.
abstract: Methanohalophilus zhilinae, a new alkaliphilic, halophilic, methylotrophic species of methanogenic bacteria, is described.  Strain WeN5T (T = type strain) from Bosa Lake of the Wadi el Natrun in Egypt was designated the type strain and was further characterized.  This strain was nonmotile, able to catabolize dimethylsulfide, and able to grow in medium with a methyl group-containing substrate (such as methanol or trimethylamine) as the sole organic compound added.  Sulfide (21 mM) inhibited cultures growing on trimethylamine.  The antibiotic susceptibility pattern of strain WeN5T was typical of the pattern for archaeobacteria, and the guanine-plus-cytosine content of the deoxyribonucleic acid was 38 mol%.  Characterization of the 16S ribosomal ribonucleic acid sequence indicated that strain WeN5T is phylogenetically distinct from members of previously described genera other than Methanohalophilus and supported the partition of halophilic methanogens into their own genus.
authors.affiliation: Division of Environmental and Occupational Health Sciences, University of California, Los Angeles 90024, USA.
authors.forename: I M
authors.initials: IM
authors.lastname: Mathrani
authors.forename: D R
authors.initials: DR
authors.lastname: Boone
authors.forename: R A
authors.initials: RA
authors.lastname: Mah
authors.forename: G E
authors.initials: GE
authors.lastname: Fox
authors.forename: P P
authors.initials: PP
authors.lastname: Lau
mesh_terms: D001105:Archaea / Q000145:classification / Q000378:metabolism / Q000502:physiology; D001483:Base Sequence; D002965:Classification; D003596:Cytosine / Q000032:analysis; D004534:Egypt; D019605:Euryarchaeota / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D006147:Guanine / Q000032:analysis; D000432:Methanol / Q000378:metabolism; D008744:Methylamines / Q000378:metabolism; D008969:Molecular Sequence Data; D012329:RNA, Bacterial / Q000032:analysis / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000032:analysis / Q000235:genetics; D013440:Sulfides / Q000378:metabolism; D014871:Water Microbiology
chemical_list: D008744:Methylamines; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D013440:Sulfides; D006147:Guanine; D003596:Cytosine; C023336:trimethylamine; C004784:dimethyl sulfide; D000432:Methanol