[Distribution of rat embryonal fibroblasts through cell cycle phases in the presence of inhibitors of active oxygen species formation and N-acetylcysteine].
Intracellular level of reactive oxygen species (ROS) and distribution of primary rat embryo fibroblast throughout the cell cycle have been studied. Serum-starved cells were activated by the addition of 10% serum to the culture medium in the presence on N-acetyl-cystein (NAC) and ROS-inhibitors, diphenileniodonium (DPI) and rothenone. It has been shown that serum starvation could block the cells at the G1/S boundary. Activation of serum-starved cells by the addition of serum reactivated the cell cycle and caused cell progression into S phase, which was paralleled with the increase in the intracellular level of ROS. Effects of NAC, PAI and rothenone, similar to that of serum starvation, blocked cell progression into S phase and decreased ROS formation due to the action of serum growth factors. The antiproliferative effect of NAC is discussed.
Gamaleĭ
I A
IA
Institute of Cytology RAS, St. Petersburg.
Polozov
Iu S
IuS
Kirpichnikova
K M
KM
Aksenov
N D
ND
Tararova
N D
ND
Pospelova
T V
TV
D016475:3T3 Cells; D000111:Acetylcysteine / Q000494:pharmacology*; D000818:Animals; D002453:Cell Cycle; D002478:Cells, Cultured; D003470:Culture Media; D004622:Embryo, Mammalian / Q000166:cytology*; D005434:Flow Cytometry; D051379:Mice; D009861:Onium Compounds / Q000494:pharmacology*; D051381:Rats; D017382:Reactive Oxygen Species; D017978:Receptors, Growth Factor / Q000378:metabolism; D012402:Rotenone / Q000494:pharmacology*; D015398:Signal Transduction
D003470:Culture Media; D009861:Onium Compounds; D017382:Reactive Oxygen Species; D017978:Receptors, Growth Factor; D012402:Rotenone; C007517:diphenyleneiodonium; D000111:Acetylcysteine