G-quadruplex DNA binding by a series of carbocyanine dyes.
We have examined a number of carbocyanine dyes for their ability to bind intramolecular G-quadruplex DNA structures (G4'-DNA) using a Taq polymerase stop assay. Of the five dyes examined, only one, N,N'-diethylthiacarbocyanine iodide (DTC), was found to bind to G4'-DNA. DTC was also the only dye found to inhibit human telomerase at 50 microM concentration.
Kerwin
S M
SM
Division of Medicinal Chemistry, College of Pharmacy, University of Texas at Austin, 78712, USA. skerwin@mail.utexas.edu
Sun
D
D
Kern
J T
JT
Rangan
A
A
Thomas
P W
PW
D001665:Binding Sites; D002232:Carbocyanines / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*; D004396:Coloring Agents / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D004353:Drug Evaluation, Preclinical; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*; D006367:HeLa Cells; D006801:Humans; D009690:Nucleic Acid Conformation; D019914:Taq Polymerase / Q000037:antagonists & inhibitors; D019098:Telomerase / Q000037:antagonists & inhibitors
D002232:Carbocyanines; D004396:Coloring Agents; D004791:Enzyme Inhibitors; C448385:N,N'-diethylthiacarbocyanine iodide; D004247:DNA; D019914:Taq Polymerase; D019098:Telomerase