Enzyme immunoassay for cuprozinc-superoxide dismutase in whole blood and urine during head down bed rest.
A highly sensitive enzyme immunoassay system for the determination of cuprozinc-superoxide dismutase (Cu, Zn-SOD) in whole blood and urine with horseradish peroxidase as a labeling enzyme was reported. It measures Cu, Zn-SOD with good repeatability of 0.05-5 ng. Coefficients of variation were 1.17%-7.94% for within-a-day assay and 1.94%-10.73% for between days assay. The average recoveries from whole blood and urine were 101.0%-113.8% and 96.0%-116.0%, respectively. Using this enzyme immunoassay, the normal range of Cu, Zn-SOD concentration in the whole blood was found to be 281.66 +/- 25.45ng/ml, and it tended to decline 3d after HDBR -6 degrees. The normal range in urine Cu, Zn-SOD concentration was 0.87 +/- 0. 33 micrograms/g of creatinine, it decreased significantly on the 3rd day during HDBR -6 degrees. Both of them recovered to normal on the 6th day during HDBR -6 degrees.
Yang
T
T
Institute of Space Medico-Engineering, Beijing, China.
Liu
Z
Z
Wang
Y
Y
D000328:Adult; D000818:Animals; D000906:Antibodies; D001510:Bed Rest; D002417:Cattle; D003404:Creatinine / Q000378:metabolism / Q000652:urine; D018475:Head-Down Tilt; D006735:Horseradish Peroxidase; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D011817:Rabbits; D013482:Superoxide Dismutase / Q000097:blood / Q000378:metabolism* / Q000652:urine
D000906:Antibodies; D003404:Creatinine; D006735:Horseradish Peroxidase; D013482:Superoxide Dismutase