The purification and properties of a cd-cytochrome nitrite reductase from Paracoccus halodenitrificans.
Paracoccus halodenitrificans, grown anaerobically in the presence of nitrite, contained membrane and cytoplasmic nitrite reductases.  When assayed in the presence of phenazine methosulfate and ascorbate, the membrane-bound enzyme produced nitrous oxide whereas the cytoplasmic enzyme produced nitric oxide.  When both enzymes were assayed in the presence of methyl viologen and dithionite, the cytoplasmic enzyme produced ammonia.  Following solubilization, the membrane-bound enzyme behaved like the cytoplasmic enzyme, producing nitric oxide in the presence of phenazine methosulfate and ascorbate, and ammonia when assayed in the presence of methyl viologen and dithionite.  The cytoplasmic and membrane-bound enzymes were purified to essentially the same specific activity.  Only a single nitrite-reductase activity was detected on electrophoretic gels and the electrophoretic behavior of both enzymes suggested they were identical.  The spectral properties of both enzymes suggested they were cd-type cytochromes.  These data suggest that the products of nitrite reduction by the cd-cytochrome nitrite reductase are determined by the location of the enzyme and the redox potential of the electron donor.
Mancinelli
R L
RL
Planetary Biology Division, Ames Research Center, Moffett Field, CA 94035, USA.
Cronin
S
S
Hochstein
L I
LI
D000641:Ammonia / Q000378:metabolism; D001426:Bacterial Proteins / Q000302:isolation & purification / Q000378:metabolism; D002462:Cell Membrane / Q000737:chemistry / Q000201:enzymology; D003580:Cytochromes / Q000302:isolation & purification* / Q000378:metabolism*; D003593:Cytoplasm / Q000737:chemistry / Q000201:enzymology; D003576:Electron Transport Complex IV / Q000302:isolation & purification / Q000378:metabolism; D008565:Membrane Proteins / Q000302:isolation & purification / Q000378:metabolism; D009572:Nitrite Reductases / Q000302:isolation & purification / Q000378:metabolism; D009609:Nitrous Oxide / Q000378:metabolism; D010230:Paracoccus / Q000737:chemistry / Q000201:enzymology*
D001426:Bacterial Proteins; D003580:Cytochromes; D008565:Membrane Proteins; D000641:Ammonia; D009572:Nitrite Reductases; C028123:nitrite reductase (NO-forming); D003576:Electron Transport Complex IV; D009609:Nitrous Oxide