Segregation of effector mechanisms in a tumour-specific CD8+ T-cell clone correlates with CD30 expression.
In this study we have analyzed CD30-antigen expression in three melanoma-directed cytotoxic T lymphocyte (CTL) clones with a T helper 0 (Th0)-like cytokine secretion profile (i.e. interleukin (IL)-4, IL-5, and interferon (IFN)-gamma). We show that all CTL clones expressed high levels of CD30 upon contact with the autologous tumour cells. One CTL clone, termed A2 with a monoclonal feature was selected for further analyses and found its CD30 expression dependent on the presence of IL-4. Functionally, a CD30-expressing A2 CTL was capable of producing higher amounts of IFN-gamma (up to 1.5-fold) and IL-4 (up to two-fold) than its CD30- counterpart. Furthermore, CD30-positive A2 CTL displayed an at least three-fold greater proliferative response to the tumour cell stimulation, contrasting with CD30- CTL. However, the antitumour cytotoxic activity of A2 CTL was not modulated by the CD30 expression. These results suggest that CD30 antigen can be inducible on a subset of tumour-directed CD8+ CTL, and that this subset of cells may have profound effector functions, such as cytokine secretion, proliferation, and cytotoxicity.
Sun
Y
Y
Skin Cancer Unit (D0900) of German Cancer Research Center, University Hospital Mannheim, D-68135 Mannheim, Germany.
Song
M
M
Maeurer
M J
MJ
Schadendorf
D
D
D002461:Cell Line, Transformed; D002999:Clone Cells; D016207:Cytokines / Q000096:biosynthesis; D003601:Cytotoxicity Tests, Immunologic; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis; D015847:Interleukin-4 / Q000096:biosynthesis / Q000494:pharmacology / Q000502:physiology; D020014:K562 Cells; D017730:Ki-1 Antigen / Q000096:biosynthesis*; D008213:Lymphocyte Activation; D008545:Melanoma / Q000276:immunology*; D010641:Phenotype; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014407:Tumor Cells, Cultured
D016207:Cytokines; D017730:Ki-1 Antigen; D015847:Interleukin-4; D007371:Interferon-gamma