Neurotrophic factor-secreting cell grafting for cerebral ischemia: preliminary report.
In this experiment, we examined a possible protective effect of encapsulated neurotrophic factor-secreting cell grafting for ischemic injury. We established a basic fibroblast growth factor (bFGF)-secreting cell line by genetic manipulation. We enveloped these cells into polymer capsules, which consist of a semipermeable membrane, and implanted them into the right striatum of rats. At 6 days after implantation, these rats received right middle cerebral artery occlusion (MCAO) using interluminal suture technique. At 24 h after MCAO, rats were sacrificed and their cerebral infarction volume was determined by 2,3,5-triphenyltetrazolium chloride (TTC) staining and image analysis. We found approximately 30% reduction in infarct volume in the encapsulated bFGF-secreting cell grafting groups vs. the encapsulated naive BHK cell grafting group or the without implantation group. We measured bFGF secretion from encapsulated bFGF-secreting cells using enzyme-linked immunosorbent assay (ELISA). The retrieved capsules continued to secrete bFGF. There was no significant difference of bFGF secretion between the capsules before and after transplantation. A large number of viable BHK-bFGF cells was observed within the full length of the retrieved capsule. These results indicate that encapsulated bFGF-secreting cell grafting exerts a protective effect on ischemic injury.
Fujiwara
K
K
Department of Neurological Surgery, Okayama University Medical School, Japan. kenjiro@gb3.so-net.ne.jp
Date
I
I
Shingo
T
T
Yoshida
H
H
Kobayashi
K
K
Takeuchi
A
A
Tamiya
T
T
Ohmoto
T
T
D000818:Animals; D002545:Brain Ischemia / Q000628:therapy*; D002214:Capsules; D002460:Cell Line; D017690:Cell Transplantation / Q000379:methods*; D006224:Cricetinae; D004797:Enzyme-Linked Immunosorbent Assay; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism*; D005818:Genetic Engineering; D020244:Infarction, Middle Cerebral Artery; D008297:Male; D011108:Polymers / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014793:Visual Cortex / Q000473:pathology / Q000601:surgery
D002214:Capsules; D011108:Polymers; D016222:Fibroblast Growth Factor 2