[Type-specific antibodies to hepatitis C virus in sera from patients with various hematologic diseases].
The reactivity of 100 sera taken from patients with different blood diseases and donors with respect to synthesized peptides in the variable area of protein NS4 of hepatitis C virus was studied. The presence of type-specific antibodies in the blood sera of patients with hepatitis C was shown. Two antigenic determinant corresponding to 1683-1705 and 1711-1732 amino acid residues in the protein area under study were detected. In hematological patients undergoing frequent blood transfusions mixed infection with different types of hepatitis C virus was registered; these types could be reliably determined with the use of synthetic peptides. The serotype determined with the use of peptides corresponded to the type of the circulating virus.
Garanzha
T A
TA
Hematological Research Center of the Russian Acad. Med. Sci., Ivanovsky Research Institute of Virology, Moscow, Russia.
Tupoleva
T A
TA
Guliaeva
A A
AA
Firsova
T V
TV
Semiletov
Iu A
IuA
Alekseenkova
T I
TI
Ianina
M V
MV
Golosova
T V
TV
Shibnev
V A
VA
Filatov
F P
FP
D000595:Amino Acid Sequence; D000914:Antibodies, Viral / Q000097:blood*; D001782:Blood Donors; D000939:Epitopes; D006402:Hematologic Diseases / Q000097:blood / Q000821:virology*; D016174:Hepacivirus / Q000276:immunology*; D006801:Humans; D015151:Immunoblotting; D007124:Immunoenzyme Techniques; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000276:immunology; D017361:Viral Nonstructural Proteins / Q000276:immunology
D000914:Antibodies, Viral; D000939:Epitopes; C093542:NS4 protein, hepatitis C virus; D010446:Peptide Fragments; D017361:Viral Nonstructural Proteins