title: Effect of vaccination with recombinant modified vaccinia virus Ankara expressing structural and regulatory genes of SIV(macJ5) on the kinetics of SIV replication in cynomolgus monkeys.
abstract: The efficacy of a multicomponent vaccination with modified vaccinia Ankara constructs (rMVA) expressing structural and regulatory genes of simian immunodeficiency virus (SIV(mac251/32H/J5)) was investigated in cynomolgus monkeys, following challenge with a pathogenic SIV. Vaccination with rMVA-J5 performed at week 0, 12, and 24 induced a moderate proliferative response to whole SIV, a detectable humoral response to all but Nef SIV antigens, and failed to induce neutralizing antibodies. Two months after the last boost, the monkeys were challenged intravenously with 50 MID50 of SIV(mac251). All control monkeys, previously inoculated with non-recombinant MVA, were infected by week two and seroconverted by weeks four to eight. In contrast a sharp increase of both humoral and proliferative responses at two weeks post-challenge was observed in vaccinated monkeys compared to control monkeys. Although all vaccinated monkeys were infected, vaccination with rMVA-J5 appeared to partially control viral replication during the acute and late phase of infection as judged by cell- and plasma-associated viral load.
authors.affiliation: Laboratory of Virology, Istituto Superiore Sanità, Rome, Italy.
authors.forename: D R
authors.initials: DR
authors.lastname: Negri
authors.forename: S
authors.initials: S
authors.lastname: Baroncelli
authors.forename: Z
authors.initials: Z
authors.lastname: Michelini
authors.forename: I
authors.initials: I
authors.lastname: Macchia
authors.forename: R
authors.initials: R
authors.lastname: Belli
authors.forename: S
authors.initials: S
authors.lastname: Catone
authors.forename: F
authors.initials: F
authors.lastname: Incitti
authors.forename: P
authors.initials: P
authors.lastname: ten Haaft
authors.forename: F
authors.initials: F
authors.lastname: Corrias
authors.forename: M P
authors.initials: MP
authors.lastname: Cranage
authors.forename: N
authors.initials: N
authors.lastname: Polyanskaya
authors.forename: S
authors.initials: S
authors.lastname: Norley
authors.forename: J
authors.initials: J
authors.lastname: Heeney
authors.forename: P
authors.initials: P
authors.lastname: Verani
authors.forename: F
authors.initials: F
authors.lastname: Titti
mesh_terms: D000818:Animals; D000917:Antibody Formation; D005260:Female; D005786:Gene Expression Regulation; D015686:Gene Products, env; D007111:Immunity, Cellular; D007262:Infusions, Intravenous; D008252:Macaca fascicularis / Q000821:virology*; D015682:Retroviridae Proteins, Oncogenic; D016097:Simian Acquired Immunodeficiency Syndrome / Q000517:prevention & control; D015302:Simian Immunodeficiency Virus / Q000472:pathogenicity*; D014611:Vaccination; D014616:Vaccinia virus / Q000235:genetics* / Q000276:immunology; D014760:Viral Fusion Proteins; D019562:Viral Load; D014765:Viral Vaccines / Q000276:immunology*; D014779:Virus Replication
chemical_list: D015686:Gene Products, env; D015682:Retroviridae Proteins, Oncogenic; D014760:Viral Fusion Proteins; D014765:Viral Vaccines; C059580:transmembrane protein, Simian immunodeficiency virus