Separation of mouse testis cells on a Celsep (TM) apparatus and their usefulness as a source of high molecular weight DNA or RNA.
The use of a self-contained unit-gravity cell separation apparatus for separation of populations of mouse testicular cells is described.  The apparatus, a Celsep (TM), maximizes the unit area over which sedimentation occurs, reduces the amount of separation medium employed, and is quite reproducible.  Cells thus isolated have been good sources for isolation of DNA, and notably, high molecular weight RNA.
Wolgemuth
D J
DJ
Department of Human Genetics and Development, Columbia University College of Physicians and Surgeons, New York, USA.
Gizang-Ginsberg
E
E
Engelmyer
E
E
Gavin
B J
BJ
Ponzetto
C
C
D000818:Animals; D002469:Cell Separation / Q000295:instrumentation* / Q000379:methods; D004247:DNA / Q000302:isolation & purification*; D004867:Equipment Design; D005069:Evaluation Studies as Topic; D008297:Male; D051379:Mice; D012313:RNA / Q000302:isolation & purification*; D013087:Spermatids / Q000166:cytology; D013091:Spermatogenesis / Q000235:genetics; D013093:Spermatogonia / Q000166:cytology; D013737:Testis / Q000166:cytology*
D012313:RNA; D004247:DNA