title: An interferon regulatory factor-like binding element restricts Xmyf-5 expression in the posterior somites during Xenopus myogenesis.
abstract: The expression of myf-5, a key component of myogenic regulatory genes, declines progressively in mature somitic cells during vertebrate myogenesis. Little is known about how this down-regulation takes place. Here we provide evidence that an interferon regulatory factor binding element (IRF element) within the Xenopus myf-5 promoter is responsible for the elimination of myf-5 transcription in mature somitic mesoderm of Xenopus embryos. We show that this IRF element mediates the down-regulation of Xmyf-5 transcription in gastrula embryos, and can specifically interact with nuclear proteins of early neurula. Moreover, deletion of this IRF element results in the anterior expansion of reporter gene transcripts within somitic mesoderm in transgenic embryos. Our results, therefore, provide insight into how the negative control of Xmyf-5 expression takes place.
authors.affiliation: Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, PR China.
authors.forename: W
authors.initials: W
authors.lastname: Mei
authors.forename: J
authors.initials: J
authors.lastname: Yang
authors.forename: Q
authors.initials: Q
authors.lastname: Tao
authors.forename: X
authors.initials: X
authors.lastname: Geng
authors.forename: R A
authors.initials: RA
authors.lastname: Rupp
authors.forename: X
authors.initials: X
authors.lastname: Ding
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001665:Binding Sites; D004268:DNA-Binding Proteins / Q000378:metabolism*; D015536:Down-Regulation; D004625:Embryo, Nonmammalian; D005775:Gastrula; D018507:Gene Expression Regulation, Developmental; D050856:Interferon Regulatory Factor-1; D008648:Mesoderm; D009124:Muscle Proteins / Q000235:genetics* / Q000378:metabolism; D051787:Myogenic Regulatory Factor 5; D009687:Nuclear Proteins / Q000378:metabolism; D010750:Phosphoproteins / Q000378:metabolism*; D012045:Regulatory Sequences, Nucleic Acid; D019170:Somites; D015534:Trans-Activators; D014981:Xenopus / Q000196:embryology / Q000235:genetics*; D029867:Xenopus Proteins
chemical_list: D004268:DNA-Binding Proteins; D050856:Interferon Regulatory Factor-1; D009124:Muscle Proteins; C497419:Myf5 protein, Xenopus; D051787:Myogenic Regulatory Factor 5; D009687:Nuclear Proteins; D010750:Phosphoproteins; D015534:Trans-Activators; D029867:Xenopus Proteins