title: Solid-phase, single nucleotide primer extension of DNA/RNA hybrids by reverse transcriptases.
abstract: A method for RNA analysis based on primer extension by reverse transcriptase is described.
authors.affiliation: Department of Chemistry, Levine Science Research Center, Duke University, Durham, NC 27708-0317, USA. pirrung@chem.duke.edu
authors.forename: M C
authors.initials: MC
authors.lastname: Pirrung
authors.forename: J D
authors.initials: JD
authors.lastname: Worden
authors.forename: J P
authors.initials: JP
authors.lastname: Labriola
authors.forename: M P
authors.initials: MP
authors.lastname: Montague-Smith
authors.forename: L J
authors.initials: LJ
authors.lastname: Weislo
mesh_terms: D020137:Base Pair Mismatch; D004247:DNA / Q000737:chemistry*; D017931:DNA Primers / Q000737:chemistry*; D004259:DNA-Directed DNA Polymerase / Q000737:chemistry / Q000378:metabolism; D015336:Molecular Probe Techniques; D016133:Polymerase Chain Reaction; D012313:RNA / Q000737:chemistry*; D012194:RNA-Directed DNA Polymerase / Q000737:chemistry* / Q000378:metabolism
chemical_list: D017931:DNA Primers; D012313:RNA; D004247:DNA; C068508:Tth polymerase; D012194:RNA-Directed DNA Polymerase; D004259:DNA-Directed DNA Polymerase