title: Synthetic peptide vaccine against Taenia solium pig cysticercosis: successful vaccination in a controlled field trial in rural Mexico.
abstract: Taenia solium cysticercosis seriously affects human health when localised in the central nervous system (CNS) and causes great economic loss in pig husbandry in rural areas of endemic countries. Increasing the resistance to the parasite in the obligatory host pig may help in curbing transmission. Three synthetic peptides based on protein sequences of the murine parasite Taenia crassiceps, which had previously been shown to induce protection in mice against homologous challenge, were tested as a vaccine against T. solium cysticercosis in pigs. Vaccinated and unvaccinated piglets (240 in all) were distributed in pairs among the peasants' households of two rural villages in Mexico in which 14% of the native pigs were cysticercotic. Ten to twelve months later, the effect of vaccination was evaluated at necropsy. Vaccination decreased the total number of T. solium cysticerci (98.7%) and reduced the prevalence (52.6%). The natural challenge conditions used in this field trial strengthen the likelihood of successful transmission control to both pig and human through a large-scale pig vaccination program. We believe this is a major contribution in anticysticercosis vaccine development as these rather simple yet protective peptides are potentially more cost-effective to produce and less variable in results than antigens that are more complex.
authors.affiliation: Facultad de Medicina, Benemérita Universidad Autónoma de Puebla, Puebla, Mexico.
authors.forename: M
authors.initials: M
authors.lastname: Huerta
authors.forename: A S
authors.initials: AS
authors.lastname: de Aluja
authors.forename: G
authors.initials: G
authors.lastname: Fragoso
authors.forename: A
authors.initials: A
authors.lastname: Toledo
authors.forename: N
authors.initials: N
authors.lastname: Villalobos
authors.forename: M
authors.initials: M
authors.lastname: Hernández
authors.forename: G
authors.initials: G
authors.lastname: Gevorkian
authors.forename: G
authors.initials: G
authors.lastname: Acero
authors.forename: A
authors.initials: A
authors.lastname: Díaz
authors.forename: I
authors.initials: I
authors.lastname: Alvarez
authors.forename: R
authors.initials: R
authors.lastname: Avila
authors.forename: C
authors.initials: C
authors.lastname: Beltrán
authors.forename: G
authors.initials: G
authors.lastname: Garcia
authors.forename: J J
authors.initials: JJ
authors.lastname: Martinez
authors.forename: C
authors.initials: C
authors.lastname: Larralde
authors.forename: E
authors.initials: E
authors.lastname: Sciutto
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003551:Cysticercosis / Q000191:economics / Q000453:epidemiology / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D004781:Environmental Exposure; D005260:Female; D008800:Mexico / Q000453:epidemiology; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000276:immunology; D010865:Pilot Projects; D011247:Pregnancy; D015995:Prevalence; D012423:Rural Health; D013552:Swine; D013553:Swine Diseases / Q000191:economics / Q000453:epidemiology / Q000276:immunology / Q000517:prevention & control*; D013621:Taenia / Q000276:immunology*; D014611:Vaccination / Q000191:economics / Q000662:veterinary*; D014612:Vaccines / Q000276:immunology*; D022223:Vaccines, Subunit / Q000276:immunology; D015047:Zoonoses
chemical_list: D010446:Peptide Fragments; D014612:Vaccines; D022223:Vaccines, Subunit