A DNA polymerase epsilon inhibitor activates the ribo and deoxyribo modes of primase expression and induces a unique phenomenon of primer accumulation.
Carbonyldiphosphonate (COMDP), a selective inhibitor of DNA polymerase (pol) epsilon, strongly stimulates expression of the ribo and deoxyribo modes of primase (Pr) activities of the Pr-DNA pol alpha enzyme complex associated with special cytoplasmic nucleoprotein complexes of chicken leukemic myeloblasts [J. Ríman and A. Sulová, Acta Virol. 41 (1997) 181-214]. Besides stimulation, COMDP uncouples the Pr activities from those of DNA pol alpha, inducing in this way a unique phenomenon of accumulation of primers of basic length. In the presence of dNTPs, the COMDP effect is counteracted by excess of mimosine. The mutually exclusive effects of these agents are discussed.
Ríman
J
J
Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Flemingovo nám. 2, 166 37 Prague 6, Czech Republic. riman@img.cas.cz
D004256:DNA Polymerase I / Q000378:metabolism; D004257:DNA Polymerase II / Q000037:antagonists & inhibitors*; D019915:DNA Primase / Q000187:drug effects / Q000378:metabolism*; D017931:DNA Primers / Q000378:metabolism*; D003854:Deoxyribonucleotides / Q000378:metabolism; D004164:Diphosphonates / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D008898:Mimosine / Q000378:metabolism; D012265:Ribonucleotides / Q000378:metabolism
D017931:DNA Primers; D003854:Deoxyribonucleotides; D004164:Diphosphonates; D004791:Enzyme Inhibitors; D012265:Ribonucleotides; C062324:carbonyldiphosphonate; D008898:Mimosine; D019915:DNA Primase; D004256:DNA Polymerase I; D004257:DNA Polymerase II