Solid-phase synthesis of oligodeoxynucleotides containing 3'-S-phosphorothiolate linkages.
For the first time a fully automated procedure has been developed for the incorporation of a 3'-S-phosphorothiolate linkage into DNA, using phosphorothioamidite monomers. Coupling yields with either of the activators 5-ethylthiotetrazole or 4,5-dicyanoimidazole were in the range of 80-90%. Coupling yields were equally good when performed on either a 0.2 or 1 mumole reaction column, thus facilitating large scale synthesis.
Fettes
K J
KJ
Department of Chemistry, University of Liverpool, Crown Street, Liverpool L69 7ZD, United Kingdom.
O'Neil
I
I
Roberts
S M
SM
Cosstick
R
R
D009841:Oligonucleotides / Q000138:chemical synthesis*; D009946:Organothiophosphorus Compounds / Q000138:chemical synthesis*; D013873:Thionucleotides / Q000138:chemical synthesis*; D013936:Thymidine / Q000031:analogs & derivatives
D009841:Oligonucleotides; D009946:Organothiophosphorus Compounds; D013873:Thionucleotides; D013936:Thymidine