Analysis of urinary prostacyclin and thromboxane/prostacyclin ratio in patients with rheumatoid arthritis using gas chromatography/selected ion monitoring.
We investigated production of prostacyclin and the urinary ratio of thromboxane and prostacyclin in patients with rheumatoid arthritis. The prostacyclin production level was assessed according to the level of urinary 2,3-dinor-6-keto-prostaglandin F(1 alpha)measuring by gas chromatography/selected ion monitoring. In patients receiving medication, the prostacyclin level was lower and the thromboxane/prostacyclin ratio was greater compare with that of healthy volunteers. The prostacyclin level in patients without medication was approximately 4-fold higher than that of healthy volunteers and 8-fold higher than those of medicated groups. Although the ratio of the group without medication was similar to that of healthy volunteers, the urinary levels of each prostanoid were higher than those of other groups. Then, the ratios of groups receiving steroids were higher than that of other groups owing to high TX level. The present findings demonstrated that endogenous prostacyclin and thromboxane production increased in patients without medication, and prostacyclin production decreased with medication.
Hishinuma
T
T
Department of Pharmaceutical Sciences, Tohoku University Hospital, Sendai, Japan
Nakamura
H
H
Sawai
T
T
Mitomo
T
T
Inoue
H
H
Matsumoto
F
F
Mizugaki
M
M
D015121:6-Ketoprostaglandin F1 alpha / Q000652:urine; D000368:Aged; D001172:Arthritis, Rheumatoid / Q000652:urine*; D002849:Chromatography, Gas / Q000379:methods*; D003404:Creatinine / Q000652:urine; D011464:Epoprostenol / Q000652:urine*; D005260:Female; D006801:Humans; D007477:Ions / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D013931:Thromboxanes / Q000652:urine*
D007477:Ions; D013931:Thromboxanes; D015121:6-Ketoprostaglandin F1 alpha; D003404:Creatinine; D011464:Epoprostenol