A DNA-binding peptide from a phage display library.
A DNA-binding peptide was selected from a random peptide phage display library. For competitive elution using the DNA methyltransferase M.TaqI in the selection step, a biotin-labeled duplex oligodeoxyribonucleotide containing the 5'-TCGA-3' recognition sequence of M.TaqI was employed. Nine of ten phages selected were found to have the same deduced amino acid sequence SVSVGMKPSPRP. The selected phage binds to DNA, as demonstrated in an ELISA.
Wölcke
J
J
Max-Planck-Instiut für molekulare Physiologie, Abteilung Physikalische Biochemie, Otto-Hahn-Str. 11, D-44227 Dortmund, Germany.
Weinhold
E
E
D000595:Amino Acid Sequence; D004247:DNA / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D009842:Oligopeptides / Q000378:metabolism*; D019151:Peptide Library; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific) / Q000378:metabolism
D004268:DNA-Binding Proteins; D009842:Oligopeptides; D019151:Peptide Library; D004247:DNA; C059680:DNA modification methylase TaqI; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific)