title: Lifetimes of bacteriochlorophyll fluorescence in Rhodopseudomonas viridis and Heliobacterium chlorum at low temperatures.
abstract: Fluorescence lifetimes of isolated membranes of Rhodopseudomonas viridis were measured in the temperature range of 77 K to 25 K.  At room temperature, the main component of the fluorescence decay of bacteriochlorophyll (BChl) b had a time constant of 50 ps.  In contrast to other purple bacteria, the emission at low temperature was spectrally homogeneous and showed essentially single lifetimes of 140 ps at 77 K and 180 ps at 25 K, with the primary electron donor in the oxidized state.  Taking into account the relative fluorescence yields with open and closed reaction centers, we arrive at numbers of 125 ps and 215 ps, respectively, for open reaction centers.  These numbers are significantly smaller than expected on the basis of measurements of the efficiency of charge separation, perhaps suggesting that the excitation decay in the absence of reaction centers is considerably faster at low temperature than at room temperature.  At least four different spectral components with different lifetimes were observed at 25 K in the emission of Heliobacterium chlorum, a short-wavelength component of about 30 ps and three longer-wavelength components of about 100 ps, 300 ps, and 900 ps.  This indicates a strong heterogeneity in the emitting pigment, BChl g-808.  The component with the shortest lifetime does not appear to be affected by the redox state of the reaction center and might reflect energy transfer to BChl g species which are connected to the reaction center.
authors.affiliation: Department of Biophysics, University of Leiden, The Netherlands.
authors.forename: F A
authors.initials: FA
authors.lastname: Kleinherenbrink
authors.forename: P
authors.initials: P
authors.lastname: Cheng
authors.forename: J
authors.initials: J
authors.lastname: Amesz
authors.forename: R E
authors.initials: RE
authors.lastname: Blankenship
mesh_terms: D001421:Bacteria, Anaerobic / Q000378:metabolism* / Q000502:physiology; D001429:Bacteriochlorophylls / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D003080:Cold Temperature; D004735:Energy Transfer; D005453:Fluorescence; D045342:Light-Harvesting Protein Complexes; D010084:Oxidation-Reduction; D018462:Photobiology; D010788:Photosynthesis / Q000502:physiology; D045322:Photosynthetic Reaction Center Complex Proteins / Q000378:metabolism; D013997:Time Factors
chemical_list: D001429:Bacteriochlorophylls; D045342:Light-Harvesting Protein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins