Ameliorative effect of vitamin E on aflatoxin-induced lipid peroxidation in the testis of mice.
OBJECTIVE
To evaluate the ameliorative effect of vitamin E on aflatoxin-induced lipid peroxidation in the testis.


METHODS
Adult male albino mice were orally administered 25 or 50 microg of aflatoxin in 0.2 mL olive oil per d for 45 d. The testis was isolated, blotted free of blood and processed for biochemical analysis.


RESULTS
There was a dose-dependent significantly higher lipid peroxidation in the testis of aflatoxin treated mice than in the controls. The levels of non-enzymatic antioxidants such as glutathione, total and reduced ascorbic acid, as well as the activities of enzymatic antioxidants, such as superoxide dismutase, glutathione peroxidase and catalase were significantly lower in the testis of aflatoxin treated mice. Vitamin E (2 mg/d per animal; orally) pretreatment significantly ameliorates the aflatoxin-induced lipid peroxidation which could be due to higher enzymatic and non-enzymatic antioxidants in the testis of mice as compared with those given aflatoxin alone.


CONCLUSIONS
Vitamin E pretreatment significantly ameliorates aflatoxin-induced lipid peroxidation in the testis of mice.
Verma
R J
RJ
Department of Zoology, University School of Sciences, Gujarat University, Ahmedabad, India. zooldeptgu@satyam.net.in
Nair
A
A
D000348:Aflatoxins / Q000494:pharmacology*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D051379:Mice; D013482:Superoxide Dismutase / Q000378:metabolism; D013737:Testis / Q000187:drug effects / Q000378:metabolism*; D014810:Vitamin E / Q000494:pharmacology*
D000348:Aflatoxins; D000975:Antioxidants; D014810:Vitamin E; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione; D001205:Ascorbic Acid