title: [Distribution of rat embryonal fibroblasts through cell cycle phases in the presence of inhibitors of active oxygen species formation and N-acetylcysteine].
abstract: Intracellular level of reactive oxygen species (ROS) and distribution of primary rat embryo fibroblast throughout the cell cycle have been studied. Serum-starved cells were activated by the addition of 10% serum to the culture medium in the presence on N-acetyl-cystein (NAC) and ROS-inhibitors, diphenileniodonium (DPI) and rothenone. It has been shown that serum starvation could block the cells at the G1/S boundary. Activation of serum-starved cells by the addition of serum reactivated the cell cycle and caused cell progression into S phase, which was paralleled with the increase in the intracellular level of ROS. Effects of NAC, PAI and rothenone, similar to that of serum starvation, blocked cell progression into S phase and decreased ROS formation due to the action of serum growth factors. The antiproliferative effect of NAC is discussed.
authors.affiliation: Institute of Cytology RAS, St. Petersburg.
authors.forename: I A
authors.initials: IA
authors.lastname: Gamaleĭ
authors.forename: Iu S
authors.initials: IuS
authors.lastname: Polozov
authors.forename: K M
authors.initials: KM
authors.lastname: Kirpichnikova
authors.forename: N D
authors.initials: ND
authors.lastname: Aksenov
authors.forename: N D
authors.initials: ND
authors.lastname: Tararova
authors.forename: T V
authors.initials: TV
authors.lastname: Pospelova
mesh_terms: D016475:3T3 Cells; D000111:Acetylcysteine / Q000494:pharmacology*; D000818:Animals; D002453:Cell Cycle; D002478:Cells, Cultured; D003470:Culture Media; D004622:Embryo, Mammalian / Q000166:cytology*; D005434:Flow Cytometry; D051379:Mice; D009861:Onium Compounds / Q000494:pharmacology*; D051381:Rats; D017382:Reactive Oxygen Species; D017978:Receptors, Growth Factor / Q000378:metabolism; D012402:Rotenone / Q000494:pharmacology*; D015398:Signal Transduction
chemical_list: D003470:Culture Media; D009861:Onium Compounds; D017382:Reactive Oxygen Species; D017978:Receptors, Growth Factor; D012402:Rotenone; C007517:diphenyleneiodonium; D000111:Acetylcysteine