title: Calbindins decreased after space flight.
abstract: Exposure of the body to microgravity during space flight causes a series of well-documented changes in Ca2+ metabolism, yet the cellular and molecular mechanisms leading to these changes are poorly understood.  Calbindins, vitamin D-dependent Ca2+ binding proteins, are believed to have a significant role in maintaining cellular Ca2+ homeostasis.  In this study, we used biochemical and immunocytochemical approaches to analyze the expression of calbindin-D28k and calbindin-D9k in kidneys, small intestine, and pancreas of rats flown for 9 d aboard the space shuttle.  The effects of microgravity on calbindins in rats from space were compared with synchronous Animal Enclosure Module controls, modeled weightlessness animals (tail suspension), and their controls.  Exposure to microgravity resulted in a significant and sustained decrease in calbindin-D28k content in the kidney and calbindin-D9k in the small intestine of flight animals, as measured by enzyme-linked immunosorbent assay (ELISA).  Modeled weightlessness animals exhibited a similar decrease in calbindins by ELISA.  Immunocytochemistry (ICC) in combination with quantitative computer image analysis was used to measure in situ the expression of calbindins in the kidney and the small intestine, and the expression of insulin in pancreas.  There was a large decrease of immunoreactivity in renal distal tubular cell-associated calbindin-D28k and in intestinal absorptive cell-associated calbindin-D9k of space flight and modeled weightlessness animals compared with matched controls.  No consistent difference in pancreatic insulin immunoreactivity between space flight, modeled weightlessness, and controls was observed.  Regression analysis of results obtained by quantitative ICC and ELISA for space flight, modeled weightlessness animals, and their controls demonstrated a significant correlation.  These findings after a short-term exposure to microgravity or modeled weightlessness suggest that a decreased expression of calbindins may contribute to the disorders of Ca2+ metabolism induced by space flight.
authors.affiliation: Department of Anatomy, Cell and Neurobiology, Marshall University School of Medicine, Huntington, WV, USA.
authors.forename: I N
authors.initials: IN
authors.lastname: Sergeev
authors.forename: W B
authors.initials: WB
authors.lastname: Rhoten
authors.forename: M D
authors.initials: MD
authors.lastname: Carney
mesh_terms: D000818:Animals; D064092:Calbindin 1; D064026:Calbindins; D002118:Calcium / Q000378:metabolism; D015870:Gene Expression; D019417:Hindlimb Suspension; D007150:Immunohistochemistry; D007328:Insulin / Q000235:genetics / Q000378:metabolism; D007421:Intestine, Small / Q000378:metabolism*; D007668:Kidney / Q000378:metabolism*; D008297:Male; D010179:Pancreas / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D064030:S100 Calcium Binding Protein G / Q000235:genetics / Q000378:metabolism*; D013026:Space Flight; D014893:Weightlessness; D018474:Weightlessness Simulation
chemical_list: C576500:Calb1 protein, rat; D064092:Calbindin 1; D064026:Calbindins; D007328:Insulin; D064030:S100 Calcium Binding Protein G; C576508:S100g protein, rat; D002118:Calcium