Formation of singlet oxygen during farmorubicin oxidation.
The enhanced generation of singlet oxygen (1O2) during oxidation of farmorubicin in the Co(II) + H2O2 system was studied using chemiluminescent, fluorescent and spectrophotometic techniques. The influence of 1O2-quenchers, catalase, superoxide anion radical (O2*-) and hydroxyl radical (HO*) scavengers on the light emission was studied. The spectrophotometric determination of 1O2 was based on bleaching of p-nitrosodimethylaniline caused by an intermediate product of the reaction of 1O2 with imidazole, and was followed by monitoring the decrease in optical density at 440 nm.
Kruk
I
I
Institute of Physics, Technical University of Szczecin, Poland. kruk@arcadia.tuniv.szczecin.pl
Michalska
T
T
Kładna
A
A
D000903:Antibiotics, Antineoplastic / Q000737:chemistry*; D015251:Epirubicin / Q000737:chemistry*; D005453:Fluorescence; D005609:Free Radicals / Q000737:chemistry; D008163:Luminescent Measurements; D055095:Optics and Photonics; D010084:Oxidation-Reduction; D010100:Oxygen / Q000737:chemistry*; D013053:Spectrophotometry
D000903:Antibiotics, Antineoplastic; D005609:Free Radicals; D015251:Epirubicin; D010100:Oxygen