title: The genome of hawaii virus and its relationship with other members of the caliciviridae.
abstract: Hawaii virus (Hu/NLV/GII/Hawaii virus/1971/US), a member of the genus 'Norwalk-like viruses' (NLVs) in the family Caliciviridae, has served as one of the reference strains for the fastidious caliciviruses associated with epidemic gastroenteritis in humans. The consensus sequence of the RNA genome of Hawaii virus was determined in order to establish its relatedness with other members of the family. The RNA genome is 7,513 nucleotides (nts) in length, excluding the 3'-end poly (A) tract, and is organized into three major open reading frames (ORFI, nts 5-5,104; ORF2, nts 5,085-6,692; and ORF3, nts 6,692-7,471). Phylogenetic analysis showed the closest relatedness of Hawaii virus throughout its genome to Lordsdale virus, a Genogroup II NLV. Analysis of the predicted secondary structure of the RNA from the 5'-end of the genome and the putative beginning of the subgenomic RNA showed the presence of two hairpin structures at both ends that are similar to each other and to those of other NLVs.
authors.affiliation: Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.
authors.forename: M A
authors.initials: MA
authors.lastname: Pletneva
authors.forename: S V
authors.initials: SV
authors.lastname: Sosnovtsev
authors.forename: K Y
authors.initials: KY
authors.lastname: Green
mesh_terms: D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D000328:Adult; D001483:Base Sequence; D002139:Caliciviridae / Q000145:classification / Q000235:genetics; D017250:Caliciviridae Infections / Q000453:epidemiology / Q000821:virology*; D002874:Chromosome Mapping; D016384:Consensus Sequence; D017124:Conserved Sequence; D010450:Endopeptidases / Q000378:metabolism; D005759:Gastroenteritis / Q000453:epidemiology / Q000821:virology*; D016679:Genome, Viral; D006254:Hawaii / Q000453:epidemiology; D006801:Humans; D008969:Molecular Sequence Data; D029322:Norovirus / Q000145:classification / Q000235:genetics*; D009690:Nucleic Acid Conformation; D016366:Open Reading Frames; D010802:Phylogeny; D012367:RNA, Viral / Q000737:chemistry; D012689:Sequence Homology, Nucleic Acid; D017361:Viral Nonstructural Proteins / Q000235:genetics
chemical_list: D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D012367:RNA, Viral; D017361:Viral Nonstructural Proteins; D010450:Endopeptidases