title: Comparison of excitability parameters and sodium channel behavior of fast- and slow-twitch rat skeletal muscles for the study of the effects of hindlimb suspension, a model of hypogravity.
abstract: When mammals are constrained to hypogravity, their neuromuscular apparatus undergoes modifications which rend difficult postural maintenance and muscular activity upon the return to normal gravitational conditions. Muscle atrophy and differetial gene expression are particularly evident in slow-twitch antigravity muscles such as the soleus. During hypogravity, most of the metabolic and contractile properties characteristic of slow-twitch muscles shift toward to those of fast-twitch muscles. For example, the expression of the fast isoforms of both the myosin heavy-chain and the sarcoplasmic reticulum calcium pump increases in slow-twitch muscle during hypogravity. Thus, modifications of the contractile machinery and calcium handling are likely to be involved in the hypogravity-induced slow-twitch muscle impariment. Fast- and slow-twitch muscles differ also in their electrical properties. Resting membrane potential (RMP) is more negative by about 10 mV in fast muscles compared to slow ones. Differences in action potential (AP) shape as well as in the number of elicitable APs have been also observed between both muscle types, which may reslut from the reported differences in chloride conductance and sodium current. Little is known about the potential modification fo muscle electrical properties during hypogravity, apart a negative shift of the RMP in soleus muscle. Thus this study was performed at the aim to compare the excitability parameters  and  sodium channel behavior of rat fast-twitch and slow-twitch muscle fibers. The characterization of these properties specific for each muscle-type will give us the basis for the study of the effect of hypogravity.
authors.affiliation: Unit of Pharmacology, Department of Pharmacobiology, University of Bari, Italy.
authors.forename: J F
authors.initials: JF
authors.lastname: Desaphy
authors.forename: S
authors.initials: S
authors.lastname: Pierno
authors.forename: A
authors.initials: A
authors.lastname: Liantonio
authors.forename: A
authors.initials: A
authors.lastname: De Luca
authors.forename: C
authors.initials: C
authors.lastname: Leoty
authors.forename: D
authors.initials: D
authors.lastname: Conte Camerino
mesh_terms: D000200:Action Potentials; D000818:Animals; D004594:Electrophysiology; D019417:Hindlimb Suspension; D008297:Male; D008564:Membrane Potentials; D008839:Microelectrodes; D018656:Muscle Fibers, Fast-Twitch / Q000502:physiology*; D018657:Muscle Fibers, Slow-Twitch / Q000502:physiology*; D018482:Muscle, Skeletal / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D015222:Sodium Channels / Q000502:physiology*; D018474:Weightlessness Simulation
chemical_list: D015222:Sodium Channels