Analysis of cytogenetic effects of the secondary radiation resulting from 70 GeV protons of Chinese hamster cells.
The cell culture of a Chinese hamster was irradiated on a Serpuchov proton synchrotron at a dose of 0.5-4 Gy and a dose rate of 1 Gy/min and by gamma-irradiation at dose 1-5 Gy and dose rate 1.2-1.4 Gy/min. The effect of radiation on the cell culture was judged from chromosomal aberrations in G2-stage of cell cycle and micronuclear test. The relative biological efficience of the secondary radiation was approximately 3. Modifying effect of caffeine on the cells irradiated by secondary radiation of synchrotron was not observed. In the presence of caffeine the effect of gamma-irradiation practically is increased up to the level observed upon secondary irradiation. This suggests that secondary radiation inhibits the repair of the cytogenetic damage.
Akhmadieva AKh
Institute of Biological Physics, USSR Academy of Sciences, Pushchino.
Aptikaeva GPh
Livanova
I A
IA
Antipov
A V
AV
Akoev
I G
IG
Ganassi
E E
EE
D000818:Animals; D002110:Caffeine / Q000494:pharmacology; D002453:Cell Cycle / Q000187:drug effects / Q000528:radiation effects*; D002460:Cell Line; D002468:Cell Physiological Phenomena / Q000187:drug effects / Q000528:radiation effects*; D002478:Cells, Cultured / Q000187:drug effects / Q000528:radiation effects*; D002869:Chromosome Aberrations; D006224:Cricetinae; D004249:DNA Damage; D004260:DNA Repair / Q000187:drug effects; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology; D011522:Protons; D011829:Radiation Dosage; D012062:Relative Biological Effectiveness; D017356:Synchrotrons
D010726:Phosphodiesterase Inhibitors; D011522:Protons; D002110:Caffeine