title: Effect of the Cymbopogon citratus, Maytenus ilicifolia and Baccharis genistelloides extracts against the stannous chloride oxidative damage in Escherichia coli.
abstract: Stannous ion has been used in different sectors of human interest, such as in food industry and in health sciences. Much is known about stannous chloride (SnCl(2)) toxicity, although, there is no general agreement regarding its genotoxicity. Cymbopogon citratus, Maytenus ilicifolia and Baccharis genistelloides extracts have been used in popular medicine. We evaluated the influence of these crude extracts on the survival of the Escherichia coli wild type (AB 1157) strain submitted to SnCl(2) treatment. Reactive oxygen species (ROS) can be generated by a Fenton like reaction induced by SnCl(2). E. coli culture was treated simultaneously with SnCl(2) and a specific extract. Our results showed a reduction of the SnCl(2) effect on the survival of the cultures in presence of the crude extracts. The extract of M. ilicifolia showed the highest level of protection action against the SnCl(2) effect in comparison with the other extracts. This protector effect could due to the redox properties of these crude extracts. The compounds in the crude extracts could (i) chelate stannous ions, protecting them against the oxidation and avoiding the generation of ROS, (ii) be a scavenger of the ROS generated by the SnCl(2) oxidation and/or (iii) have oxidant compounds that could oxidise the stannous ions, abolishing or reducing the SnCl(2) effect.
authors.affiliation: Departamento de Biofisica e Biometria, Instituto de Biologia Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro, Av 28 de setembro, 87 20551-030, RJ, Rio de Janeiro, Brazil.
authors.forename: S F
authors.initials: SF
authors.lastname: Melo
authors.forename: S F
authors.initials: SF
authors.lastname: Soares
authors.forename: R F
authors.initials: RF
authors.lastname: da Costa
authors.forename: C R
authors.initials: CR
authors.lastname: da Silva
authors.forename: M B
authors.initials: MB
authors.lastname: de Oliveira
authors.forename: R J
authors.initials: RJ
authors.lastname: Bezerra
authors.forename: A
authors.initials: A
authors.lastname: Caldeira-de-Araújo
authors.forename: M
authors.initials: M
authors.lastname: Bernardo-Filho
mesh_terms: D016587:Antimutagenic Agents / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity; D010936:Plant Extracts / Q000494:pharmacology*; D010946:Plants, Medicinal; D006109:Poaceae / Q000737:chemistry* / Q000145:classification; D017382:Reactive Oxygen Species; D013014:SOS Response, Genetics / Q000187:drug effects*; D013045:Species Specificity; D017971:Tin Compounds / Q000633:toxicity
chemical_list: D016587:Antimutagenic Agents; D009153:Mutagens; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D017971:Tin Compounds; C023599:stannous chloride