title: Synthesis of beta-D-glucosyl- and beta-D-fucosyl-glucoses using beta-glycosidase from Thermus thermophilus.
abstract: The thermostable beta-glycosidase from Thermus thermophilus, cloned and overexpressed in Escherichia coli was used to catalyze the transfer of beta-D-glucosyl and beta-D-fucosyl from the corresponding p-nitrophenyl-beta-D-glycopyranosides to a hydroxyl group of glucose in the synthesis of beta-D-glucosyl-D-glucopyranoses and beta-D-fucosyl-D-glucopyranoses. The yields in disaccharides produced under conditions of non-initial velocity were very attractive and the formation of the beta(1-3) linked disaccharides was largely favored. The enzyme could constitute a valuable biocatalyst for the synthesis of disaccharides involving such structures as, for example, Bifidus factors.
authors.affiliation: Laboratoire de Biochimie, Unité de Recherche sur la Biocatalyse, CNRS 2230, Faculté des Sciences et des Techniques, Université de Nantes, France.
authors.forename: L
authors.initials: L
authors.lastname: Fourage
authors.forename: B
authors.initials: B
authors.lastname: Colas
mesh_terms: D004187:Disaccharides / Q000096:biosynthesis*; D019075:Electrophoresis, Capillary; D006026:Glycoside Hydrolases / Q000378:metabolism*; D006031:Glycosylation; D009005:Monosaccharides / Q000378:metabolism / Q000494:pharmacology; D016963:Thermus thermophilus / Q000201:enzymology*; D005644:alpha-L-Fucosidase / Q000378:metabolism; D001617:beta-Glucosidase / Q000378:metabolism*
chemical_list: D004187:Disaccharides; D009005:Monosaccharides; D006026:Glycoside Hydrolases; D001617:beta-Glucosidase; D005644:alpha-L-Fucosidase