title: Cloning and sequencing of a gene encoding a novel salt stress-induced membrane protein from Rhodobacter sphaeroides f. sp. dentrificans.
abstract: The gene encoding a membrane protein, SspA, induced under salt stress conditions was cloned and sequenced from a photosynthetic bacterium, Rhodobacter sphaeroides f. sp. denitrificans IL106. A single open reading frame consisting of 972 base pairs that encoded a polypeptide composed of a signal peptide of 24 amino acids and a mature protein of 300 amino acids (Mr 33,386) was found. A database search failed to detect any highly homologous sequences, indicating that SspA is a novel protein. The protein was present in the outer membrane as a transmembrane protein and was specifically induced by salt stress, but not by heat shock.
authors.affiliation: Department of Applied Biological Chemistry, The University of Tokyo, Japan.
authors.forename: X Y
authors.initials: XY
authors.lastname: Xu
authors.forename: H
authors.initials: H
authors.lastname: Kadokura
authors.forename: A
authors.initials: A
authors.lastname: Okubo
authors.forename: K
authors.initials: K
authors.lastname: Kitamoto
authors.forename: S
authors.initials: S
authors.lastname: Yamazaki
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D001483:Base Sequence; D003001:Cloning, Molecular; D006360:Heat-Shock Proteins / Q000235:genetics* / Q000378:metabolism; D018869:Heat-Shock Response; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D012242:Rhodobacter sphaeroides / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000494:pharmacology*
chemical_list: D001426:Bacterial Proteins; D006360:Heat-Shock Proteins; D008565:Membrane Proteins; C446279:SspA protein, Rhodobacter sphaeroides; D012965:Sodium Chloride