Semiautomated and simultaneous analysis of the interleukin-10 gene microsatellites IL-10G and IL-10R by fluorescence-based polymerase chain reaction reveals significant differences in allele distributions between Caucasians (Germany) and Africans (Gabon).
Interleukin-10 (IL-10) is an important immunoregulatory cytokine influencing many aspects of the adaptive and inflammatory immune response. Two dinucleotide repeats have been identified in the 5'-UTR of IL-10 and shown to be useful genetic markers in several diseases. A simple, two-colour fluorescence assay was developed for determination of microsatellite fragment length by an automatic sequencer. Using this method polymorphisms at the IL-10G and IL-10R loci of the 5' flanking region of the IL-10 gene can be identified simultaneously. A unified standard nomenclature was applied to the known IL-10G and IL-10R microsatellites. The multiplex PCR approach was used to compare the allele frequencies in two independent donor groups from Germany (Caucasian), comprising 112 and 106 cases, respectively, and one group from Gabon (African) including 91 donors. Significant differences in the allele distribution were found. Both Caucasian populations tested showed no significant differences in their allele and genotype distribution. Whereas in Africans, allele IL-10G25 is rare at 3% compared to 21% in Caucasian, alleles IL-10G22 and G23 are more prevalent in Africans than in Caucasians (22% versus 10% and 26% versus 7%, respectively). Within the IL-10R locus, the allele R13 was observed at 88% in the African group compared to 69% in Caucasians. These data may help immunogenetic studies in diseases, where IL-10 is thought to be deregulated.
Kube
D
D
Eberhard-Karls-Universität Universitätsklinikum, Institut für Tropenmedizin Sektion Humanparasitologie, Wilhelmstrasse 27 D-72074 Tübingen, Germany. dieter.kube@uni-tuebingen.de
Schmidt
D
D
Mörmann
M
M
Uhlemann
A C
AC
Tomiuk
J
J
Tesch
H
H
Kremsner
P G
PG
D000483:Alleles; D044383:Black People / Q000235:genetics*; D004247:DNA / Q000302:isolation & purification*; D005260:Female; D005453:Fluorescence; D005681:Gabon; D005787:Gene Frequency / Q000235:genetics; D005819:Genetic Markers / Q000235:genetics; D015894:Genome, Human; D005838:Genotype; D005858:Germany; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics*; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic / Q000235:genetics; D044465:White People / Q000235:genetics*
D005819:Genetic Markers; D016753:Interleukin-10; D004247:DNA