Concept of immunomics: a new frontier in the battle for gene function?
At the beginning of the 21st century, biology will try to address the function of a large number of new genes. From the perspective of technologies applied today to functional genomics, this task appears to be more complex than the effort invested in the sequencing of the human genome. Conceptually, a high-throughput approach permitting correlation between newly discovered genes and functional properties of their protein products has yet to be developed. To address relationships between tens of thousands of genes and their cognate proteins, novel interdisciplinary technologies need to emerge. In this paper, a new idea of immunomics is presented and an experimental strategy is outlined to circumvent some of the restrictions associated with methodologies currently in use. It is proposed that cloned segments of genomic DNA are used for genetic immunization to obtain a large collection of antibodies, and to generate microarrays of these antibodies for tracing differentially expressed cellular proteins.
Klysik
J
J
Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA. jank@bcm.tmc.edu
D000818:Animals; D000906:Antibodies / Q000235:genetics* / Q000276:immunology; D015894:Genome, Human; D006801:Humans; D007114:Immunization / Q000379:methods; D011506:Proteins / Q000235:genetics* / Q000276:immunology*; D012306:Risk; D019444:Vaccines, DNA / Q000235:genetics / Q000276:immunology
D000906:Antibodies; D011506:Proteins; D019444:Vaccines, DNA