Anion-exchange high-performance liquid chromatography with post-column detection for the analysis of phytic acid and other inositol phosphates.
The use of gradient anion-exchange HPLC, with a simple post-column detection system, is described for the separation of myo-inositol phosphates, including "phytic acid" (myo-inositol hexaphosphate). Hexa-, penta-, tetra-, tri- and diphosphate members of this homologous series are clearly resolved within 30 min. This method should facilitate analysis and quantitation of "phytic acid" and other inositol phosphates in plant, food, and soil samples.
Rounds
M A
MA
Department of Food Science, Purdue University, West Lafayette, IN 47907-1160, USA.
Nielsen
S S
SS
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002852:Chromatography, Ion Exchange / Q000379:methods*; D005069:Evaluation Studies as Topic; D005504:Food Analysis; D007295:Inositol Phosphates / Q000032:analysis / Q000302:isolation & purification*; D010833:Phytic Acid / Q000032:analysis / Q000302:isolation & purification*; D010944:Plants / Q000737:chemistry; D012987:Soil / Q000032:analysis
D007295:Inositol Phosphates; D012987:Soil; D010833:Phytic Acid