title: Effect on polymorphonuclear cell function of a human-specific cytotoxin, intermedilysin, expressed by Streptococcus intermedius.
abstract: Streptococcus intermedius is a member of the normal flora of the mouth but is also an opportunistic pathogen associated with purulent infections at oral and nonoral sites. Intermedilysin (ILY) has been shown to be a cytolysin capable of generating pores in the cell membrane of erythrocytes demonstrable by electron microscopy. This effect has been shown to be specific for human cells. Since polymorphonuclear cells (PMNs) are the main cell involved in innate immunity we investigated the effect of purified intermedilysin from Streptococcus intermedius on PMN function. Active ILY at a concentration of 40 ng/microl caused a significant decrease in the number of intact PMNs after 60 min. The active cytolysin, when compared with heat-inactivated ILY, did not appear to be chemotactic for the PMNs but did cause an increase in intracellular calcium, with increased cell surface CD11b expression, metabolic burst, and phagocytosis of Staphylococcus aureus. These findings may have implications for the role of ILY in deep-seated abscesses.
authors.affiliation: Department of Haematology, Barts and the London NHS Trust, United Kingdom. macey.marion@royalhos-tr.nthames.nhs.uk
authors.forename: M G
authors.initials: MG
authors.lastname: Macey
authors.forename: R A
authors.initials: RA
authors.lastname: Whiley
authors.forename: L
authors.initials: L
authors.lastname: Miller
authors.forename: H
authors.initials: H
authors.lastname: Nagamune
mesh_terms: D001426:Bacterial Proteins; D001427:Bacterial Toxins / Q000494:pharmacology*; D001430:Bacteriocins; D002118:Calcium / Q000378:metabolism; D002459:Cell Fusion; D002463:Cell Membrane Permeability; D048429:Cell Size; D002634:Chemotaxis, Leukocyte / Q000276:immunology; D003603:Cytotoxins / Q000494:pharmacology*; D005434:Flow Cytometry / Q000379:methods; D006461:Hemolysis; D006801:Humans; D007424:Intracellular Fluid / Q000378:metabolism; D007958:Leukocyte Count; D016177:Macrophage-1 Antigen / Q000096:biosynthesis; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D010587:Phagocytosis / Q000276:immunology; D013211:Staphylococcus aureus / Q000276:immunology; D013291:Streptococcus
chemical_list: D001426:Bacterial Proteins; D001427:Bacterial Toxins; D001430:Bacteriocins; D003603:Cytotoxins; D016177:Macrophage-1 Antigen; C403910:intermedilysin protein, Streptococcus intermedius; D002118:Calcium