A potentialgradient-conductivity-scanner for the investigation of effects leading to buoyancy-driven convection on continuous-flow-electrophoresis.
Continuous flow electrophoresis (CFE) is a mild and high-resolving method for the separation of sensitive biomolecules like peptides and proteins.  The resolution is decreased by several effects, of which density gradients are important since they lead to buoyancy-driven convection.  A tool for the measurement of physical quantities that cause the separation process, a potentialgradient/conductivity-scanner has been developed.  With this PC-scanner consecutive potential- and concentration profiles can be measured inside the CFE-chamber.  Local current densities and local joule heat yielding density gradients are calculated from the measurement data.  With the PC-scanner the influence of gravity on separations by CFE can be evaluated more easily by the knowledge of the thermal and electrical conditions inside the separation slit.
Heinrich
J
J
Institut für Anorganische und Analytische Chemie und Radiochemie, Universität des Saarlandes, Saarbrücken, Germany.
Wagner
H
H
D001709:Biotechnology; D018481:Convection; D004553:Electric Conductivity; D004573:Electrolytes; D004586:Electrophoresis / Q000379:methods*; D004867:Equipment Design; D006358:Hot Temperature; D008564:Membrane Potentials; D008567:Membranes, Artificial; D010455:Peptides / Q000032:analysis; D011506:Proteins / Q000032:analysis; D013026:Space Flight; D014893:Weightlessness
D004573:Electrolytes; D008567:Membranes, Artificial; D010455:Peptides; D011506:Proteins