The structure of a polysaccharide from infectious strains of Burkholderia cepacia.
The structure of an acidic exopolysaccharide (EPS) from eight strains of Burkholderia cepacia has been investigated by methylation and sugar analysis, periodate oxidation-Smith degradation, and partial acid-hydrolysis. An enzyme preparation obtained from the same organisms producing the EPS was also used to depolymerize the polysaccharide. Detailed NMR studies of the chemical and enzymatic degradation products showed that this EPS consists of a highly branched heptasaccharide-repeating unit with the following structure: [abstract: see text]. About three O-acetyl groups per repeating unit are present at undetermined positions.
Linker
A
A
Veterans Affairs (VA), Salt Lake City Health Care System, 500 Foothill Boulevard (151E), Salt Lake City, UT 84124, USA.
Evans
L R
LR
Impallomeni
G
G
D000818:Animals; D001704:Biopolymers / Q000737:chemistry / Q000378:metabolism; D016956:Burkholderia cepacia / Q000737:chemistry* / Q000145:classification* / Q000201:enzymology / Q000502:physiology; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002417:Cattle; D005949:Glucose Oxidase / Q000378:metabolism; D005966:Glucuronidase / Q000378:metabolism; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D010084:Oxidation-Reduction; D010504:Periodic Acid / Q000378:metabolism; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000378:metabolism
D001704:Biopolymers; D011135:Polysaccharides, Bacterial; D010504:Periodic Acid; C009288:metaperiodate; D005949:Glucose Oxidase; D005966:Glucuronidase