title: Protein kinase D interacts with Golgi via its cysteine-rich domain.
abstract: Protein kinase D (PKD)/protein kinase Cmu is a serine/threonine protein kinase that has been localized in the cytosol and in several intracellular compartments including Golgi, mitochondria and plasma membrane. Using real time imaging of fluorescent protein (GFP)-tagged PKD, we have found that the accumulation of PKD in the Golgi compartment, following a temperature shift from 37 to 20 degrees C, was mediated by the cysteine-rich domain (CRD) of PKD. The CRD of PKD also mediates its interaction with the plasma membrane, further supporting the conclusion that the CRD of PKD may act as a subcellular localization signal.
authors.affiliation: Unit of Signal Transduction and Gastrointestinal Cancer, Division of Digestive Diseases, UCLA School of Medicine, Los Angeles, CA 90095-1786, USA.
authors.forename: O
authors.initials: O
authors.lastname: Rey
authors.forename: E
authors.initials: E
authors.lastname: Rozengurt
mesh_terms: D016475:3T3 Cells; D000818:Animals; D002384:Catalysis; D003545:Cysteine / Q000378:metabolism*; D006056:Golgi Apparatus / Q000378:metabolism*; D051379:Mice; D011493:Protein Kinase C / Q000737:chemistry / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D013347:Subcellular Fractions
chemical_list: C089212:protein kinase D; D011493:Protein Kinase C; D003545:Cysteine