title: Combined treatment with vitamin B12b and ascorbic acid causes in vitro DNA degradation in tumor cells.
abstract: Incubation of Ehrlich ascites carcinoma and HEp-2 human epidermoid laryngeal carcinoma cells with hydroxycobalamin (vitamin B12b) and ascorbic acid induced generation and accumulation of double-stranded DNA fragments (23,000 b.p. and longer) in cells. The same vitamins alone in the same concentrations produced no such effects. DNA degradation in HEp-2 cells caused by long-term (4 h) incubation with 5-25 microM hydroxycobalamin and ascorbic acid (1:10-1:40 molar ratio) at 37 degrees C was comparable with that induced by gamma-irradiation in a dose of 150 Gy at 4 degrees C.
authors.affiliation: Laboratory of Cell Biochemistry and Bioenergetics, Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino.
authors.forename: A I
authors.initials: AI
authors.lastname: Medvedev
authors.forename: V S
authors.initials: VS
authors.lastname: Akatov
authors.forename: N D
authors.initials: ND
authors.lastname: Kreshchenko
authors.forename: M E
authors.initials: ME
authors.lastname: Solov'eva
authors.forename: V V
authors.initials: VV
authors.lastname: Leshchenko
authors.forename: E I
authors.initials: EI
authors.lastname: Lezhnev
authors.forename: R I
authors.initials: RI
authors.lastname: Yakubovskaya
mesh_terms: D001205:Ascorbic Acid / Q000494:pharmacology*; D004249:DNA Damage; D004273:DNA, Neoplasm / Q000187:drug effects*; D003851:Deoxyribonucleases / Q000494:pharmacology*; D004357:Drug Synergism; D006801:Humans; D006879:Hydroxocobalamin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
chemical_list: D004273:DNA, Neoplasm; D003851:Deoxyribonucleases; D001205:Ascorbic Acid; D006879:Hydroxocobalamin