Isolation of chlamydia psittaci from domestic cats with oculonasal discharge in Japan.
Eight strains of Chlamydia psittaci were isolated in Japan from the nasal and conjunctival swabs of six household cats using the L929 cell line of mouse fibroblast origin. The isolates were identified as C. psittaci on the basis of the formation of characteristic inclusion bodies in the cell culture detected by Giemsa stain and immunofluorescence. Comparison of nucleotide sequences of the ompA gene amplified from the three isolates with the published sequence of feline FEPN strain of C. psittaci showed almost 100% homology.
Iwamoto
K
K
Kyoto Biken Laboratories, Inc, Uji, Japan.
Masubuchi
K
K
Nosaka
H
H
Kokubu
T
T
Nishida
K
K
Toshida
T
T
Yamanaka
M
M
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis / Q000096:biosynthesis; D002371:Cat Diseases / Q000382:microbiology*; D002415:Cats; D002478:Cells, Cultured; D002690:Chlamydia Infections / Q000382:microbiology / Q000662:veterinary*; D002691:Chlamydophila psittaci / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D003231:Conjunctivitis / Q000382:microbiology / Q000662:veterinary; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D002479:Inclusion Bodies / Q000382:microbiology; D051379:Mice; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012220:Rhinitis / Q000382:microbiology / Q000662:veterinary
D000907:Antibodies, Bacterial; D004269:DNA, Bacterial