Structural and promoter regions of the murine pyruvate carboxylase gene.
We have cloned and sequenced the gene encoding mouse pyruvate carboxylase (mPC) [EC 6.4.1.1]. The coding region contains 19 exons, one 5'-untranslated region exon, and 19 introns in 22 kb of genomic DNA. This gene's exon/intron organization is highly conserved with respect to rat and human PC genes. The mPC gene promoter lacks canonical TATA and CCAAT boxes, in common with a number of housekeeping genes. Transient expressions in COS-1 of a luciferase reporter gene under the control of 5'-nested deletions of the 5'-flanking sequence of the mPC gene have identified the 166-bp minimal sequence required for basal transcription. Alternative splicing at the 5'-untranslated region exon of the mouse PC gene results in the production of two alternate transcripts bearing different 5'-noncoding regions. Both transcripts are highly expressed in kidney and liver and moderately expressed in heart and testis and expressed at a low level in spleen.
Jitrapakdee
S
S
Department of Biochemistry, Faculty of Science, Mahidol University, Phya Thai, Bangkok 10400, Thailand. scsji@mahidol.ac.th
Petchamphai
N
N
Sunyakumthorn
P
P
Wallace
J C
JC
Boonsaeng
V
V
D000818:Animals; D001483:Base Sequence; D005091:Exons; D005786:Gene Expression Regulation; D016678:Genome; D007438:Introns; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009928:Organ Specificity; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011766:Pyruvate Carboxylase / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism
D012333:RNA, Messenger; D011766:Pyruvate Carboxylase