A panel of eight tests in the serodiagnosis and immunological evaluation of acute brucellosis.
A panel of eight tests was used to study 200 cases of acute brucellosis, 200 patients negative for brucella as a control group and 200 apparently healthy individuals as a second control group. The best diagnostic test was the rose Bengal test using an imported reagent (BioMérieux, France) and 2 local reagents. This test was improved from being a screening test to be a titrable one. The best two tests used together were the tube agglutination test with Coomb-like test. The indirect fluorescent antibody test had no advantages over the use of other tests. The 2-mercaptoethanol test and C-reactive protein test were useful in checking the brucellosis activity. Normal numbers of E-rosette forming cells and inefficient neutrophils in phagocytosis were found in peripheral blood during acute brucellosis.
Dabdoob
W A
WA
Department of Microbiology, College of Medicine, University of Mosul, Mosul, Iraq.
Abdulla
Z A
ZA
D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D000368:Aged; D000372:Agglutination Tests / Q000379:methods* / Q000592:standards; D002006:Brucellosis / Q000097:blood* / Q000175:diagnosis* / Q000276:immunology; D002097:C-Reactive Protein / Q000378:metabolism; D016022:Case-Control Studies; D003298:Coombs Test / Q000379:methods* / Q000592:standards; D019084:Fluorescent Antibody Technique, Indirect / Q000379:methods* / Q000592:standards; D006801:Humans; D007111:Immunity, Cellular / Q000276:immunology; D007958:Leukocyte Count; D008403:Mass Screening / Q000379:methods / Q000592:standards; D008623:Mercaptoethanol; D008875:Middle Aged; D009504:Neutrophils / Q000276:immunology; D012680:Sensitivity and Specificity; D013601:T-Lymphocytes / Q000276:immunology
D008623:Mercaptoethanol; D002097:C-Reactive Protein