Reduced reproduction with increased abortion rate in transgenic mice that overexpress 15-lipoxygenase.
15-Lipoxygenase (15-LOX) is a lipid-oxidizing enzyme that is involved in cell cycle regulation. To evaluate the effect of 15-LOX on reproduction, we studied transgenic mice that overexpress 15-LOX. The transgene was introduced over the genetic background of the low density lipoprotein receptor deficient mice (LDL-R(-/-)) and reproduction was compared to LDL-R(-/-) mice. We found a lower pregnancy rate in the 15-LOX/LDL-R(-/-) mice as compared to the LDL-R(-/-) mice (62.74 vs. 79.1%, p < 0.01). Additionally, a remarkably higher number of resorptions per pregnancy was found in the 15-LOX/LDL-R(-/-) mice (16.7 vs. 3.27%, p < 0.001) and it was accompanied by a significantly higher activity of the 15-LOX enzyme in these resorptions as compared to other tissues. These findings may implicate a role for 15-LOX in the development of spontaneous abortions.
Dar
P
P
Department of Obstetrics and Gynecology, Division of Reproductive Genetics, Albert Einstein College of Medicine, Bronx, NY, USA. peerdar@yahoo.com
Strassburger
D
D
Shaish
A
A
Levkovitz
H
H
Halperin
R
R
Harats
D
D
D000022:Abortion, Spontaneous / Q000378:metabolism*; D000818:Animals; D001093:Arachidonate 15-Lipoxygenase / Q000378:metabolism*; D001723:Birth Rate; D005260:Female; D008098:Litter Size; D051379:Mice; D008810:Mice, Inbred C57BL / Q000378:metabolism*; D008822:Mice, Transgenic / Q000378:metabolism*; D023421:Models, Animal; D011247:Pregnancy; D011973:Receptors, LDL / Q000378:metabolism
D011973:Receptors, LDL; D001093:Arachidonate 15-Lipoxygenase