Investigation of binding process of chlorpromazine to bovine serum albumin by means of passive and active experiments.
The association process of chlorpromazine with bovine serum albumin by both onefactoral passive and multifactoral active experiments were investigated in model systems. The dependence of absolute concentration of the free fraction of chlorpromazine on the total concentration of chlorpromazine is described by the increasing function, but the dependence of the relative concentration (free/total) on this factor is influenced by external characteristics. The concentrations of chlorpromazine, bovine serum albumin and Tritons' mixture, as principal factors of a multifactoral experiment, influence the absolute and relative values of the free fraction of chlorpromazine non-additively, ie the interaction of factors is significant.
Rukhadze
M D
MD
Department of Chemistry, Tbilisi State University, Tbilisi 380028, Georgia.
Bezarashvili
G S
GS
Sidamonidze
N S
NS
Tsagareli
S K
SK
D000818:Animals; D014150:Antipsychotic Agents / Q000378:metabolism*; D002417:Cattle; D002746:Chlorpromazine / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D011485:Protein Binding; D012710:Serum Albumin, Bovine / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet
D014150:Antipsychotic Agents; D012710:Serum Albumin, Bovine; D002746:Chlorpromazine