Calcium ion involvement in growth inhibition of mechanically stressed soybean (Glycine max) seedlings.
A 40-50% reduction in soybean [Glycine max (L.) Merr. cv. Century 84] hypocotyl elongation occurred 24 h after application of mechanical stress.  Exogenous Ca2+ at 10 mM inhibited growth by 28% if applied with the Ca2+ ionophore A23187 to the zone of maximum hypocotyl elongation.  La3+ was even more inhibitory than Ca2+, especially above 5 mM.  Treatment with ethyleneglycol-bis-(beta-aminoethylether)-N, N, N', N'-tetraacetic acid (EGTA) alone had no effect on growth of non-stressed seedlings at the concentrations used but negated stress-induced growth reduction by 36% at 4 mM when compared to non-treated, stressed controls.  Treatment with EDTA was ineffective in negating stress-induced growth inhibition.  Calmodulin antagonists calmidazolium, chlorpromazine, and 48/80 also negated stress-induced growth reduction by 23, 50, and 35%, respectively.
Jones
R S
RS
Dept. of Horticulture, Purdue Univ., West Lafayette, IN 47907, USA.
Mitchell
C A
CA
D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism* / Q000502:physiology; D002147:Calmodulin / Q000037:antagonists & inhibitors / Q000502:physiology; D002413:Cations, Divalent; D002614:Chelating Agents / Q000494:pharmacology; D002746:Chlorpromazine / Q000494:pharmacology; D003624:Darkness; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D004533:Egtazic Acid / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D018546:Hypocotyl / Q000187:drug effects* / Q000254:growth & development*; D007093:Imidazoles / Q000494:pharmacology; D007476:Ionophores / Q000494:pharmacology; D007811:Lanthanum / Q000494:pharmacology; D010812:Physical Stimulation / Q000009:adverse effects; D013025:Glycine max / Q000187:drug effects* / Q000254:growth & development; D013314:Stress, Mechanical
D002147:Calmodulin; D002413:Cations, Divalent; D002614:Chelating Agents; D004791:Enzyme Inhibitors; D007093:Imidazoles; D007476:Ionophores; D000001:Calcimycin; C031938:calmidazolium; D004533:Egtazic Acid; D007811:Lanthanum; D004492:Edetic Acid; D002118:Calcium; D002746:Chlorpromazine; D004121:Dimethyl Sulfoxide