Effects of simulated weightlessness and gamma-irradiation on myocardial cells and osteoblasts.
The effects of simulated weightlessness and gamma-irradiation on the structure and function of the myocardial cells and osteoblasts were investigated with a rotating clinostat and 2 Gy 60Co gamma-irradiation. The results showed: (1) Under the simulated weightlessness (SWL), the diameter of the myocardial cells decreased 40%. And the long-short diameter proportion decreased 70%, (P < 0.01); SWL affected the cell skeleton and the distribution of microfilament became ununiform, the orientation was unclear; SWL had significant effects on osteoblast disintegration and proliferation. The disintegration function decreased and the proliferation process was inhibited; (2) 2 Gy 60Co gamma-ray irradiation had significant effects on Lactate Dehydrogenase (LDH) activity and Superoxide Dismutase (SOD), Malonaldehyde (MDA) content in the myocardial cells, (P < 0.05) (5) A synergistic effect was observed between irradiation and SWL.
Zhang
X
X
Institute of Space Medico-Engineering, Beijing, China.
Wang
G
G
Ding
B
B
Yue
M
M
Tan
Y
Y
Zeng
Y
Y
D000818:Animals; D002455:Cell Division / Q000528:radiation effects; D048429:Cell Size; D005720:Gamma Rays; D006321:Heart / Q000528:radiation effects; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D009206:Myocardium / Q000166:cytology* / Q000201:enzymology; D010006:Osteoblasts / Q000166:cytology* / Q000201:enzymology / Q000528:radiation effects*; D051381:Rats; D017208:Rats, Wistar; D012399:Rotation; D013482:Superoxide Dismutase / Q000378:metabolism; D018474:Weightlessness Simulation
D008315:Malondialdehyde; D007770:L-Lactate Dehydrogenase; D013482:Superoxide Dismutase