EDTA-induced pseudo-neutropenia resolved with kanamycin.
This report describes a case of spurious neutropenia caused by EDTA-dependent in vitro agglutination of neutrophils. After raising the temperature of the sample to 37 degrees C the agglutination was irreversible, but it resolved completely after addition of kanamycin. Previously this method has been shown to be effective in EDTA-dependent pseudo-thrombocytopenia, but this is the first report demonstrating successful application in EDTA-dependent pseudo-neutropenia.
Hoffmann
J J
JJ
Department of Clinical Laboratories, Haematology Division, Catharina Hospital, Eindhoven, The Netherlands.
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001800:Blood Specimen Collection / Q000379:methods / Q000592:standards; D002448:Cell Adhesion / Q000187:drug effects; D004492:Edetic Acid / Q000009:adverse effects*; D005189:False Positive Reactions; D006384:Hemagglutination / Q000187:drug effects; D006801:Humans; D007612:Kanamycin / Q000494:pharmacology*; D008297:Male; D008875:Middle Aged; D009503:Neutropenia / Q000139:chemically induced* / Q000175:diagnosis; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects
D000900:Anti-Bacterial Agents; D007612:Kanamycin; D004492:Edetic Acid