title: A method for the imbibition and germination of wheat seeds in space.
abstract: A method was developed for the reliable germination in space of wheat seeds on porous tube nutrient delivery systems. Germination paper strips were loosely rolled into cylinders and two seeds inserted close to the outer edges of each cylinder. This configuration: 1) directed the emerging shoots upward and roots downward, 2) was efficient in wicking moisture from the porous tubes, and 3) provided open areas for oxygen diffusion. Cotton tufts were inserted into the bottom crevices of the cylinders to fix the seeds in a mid-level position and cylinders were then storable (indefinitely) prior to the preprogrammed (on-orbit) initiation of imbibition. This method extends both the upper and lower ends of acceptable moisture levels for successful seed germination, increasing the probability of success for spaceflight applications where moisture availability is more variable than on Earth.
authors.affiliation: Gravitational Biology Laboratory, Dynamic Corporation, Kennedy Space Center, FL 32899, USA.
authors.forename: H G
authors.initials: HG
authors.lastname: Levine
authors.forename: W C
authors.initials: WC
authors.lastname: Piastuch
mesh_terms: D003470:Culture Media; D015194:Diffusion Chambers, Culture; D018504:Ecological Systems, Closed; D004867:Equipment Design; D018525:Germination; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism / Q000382:microbiology; D012639:Seeds / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism / Q000382:microbiology; D012973:Sodium Hypochlorite / Q000494:pharmacology; D018531:Spacecraft / Q000295:instrumentation*; D013242:Sterilization; D013696:Temperature; D013997:Time Factors; D014908:Triticum / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism / Q000382:microbiology; D014867:Water / Q000378:metabolism*
chemical_list: D003470:Culture Media; D014867:Water; D012973:Sodium Hypochlorite