Change in the degree of adsorption of proteins by aluminum-containing adjuvants following exposure to interstitial fluid: freshly prepared and aged model vaccines.
The ability of interstitial fluid to change the degree of adsorption of ovalbumin to aluminum hydroxide adjuvant or lysozyme to aluminum phosphate adjuvant was studied. Ovalbumin and lysozyme were almost completely eluted after exposure at 37 degrees C to sheep lymph fluid for 4h or 15 min, respectively. The ability of sheep lymph fluid to elute lysozyme from aluminum phosphate adjuvant did not change as the model vaccine aged. However, only 60% of the ovalbumin adsorbed to aluminum hydroxide adjuvant was eluted during exposure to sheep lymph fluid for 24h after the model vaccine aged for 11 weeks at 4 degrees C.
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Department of Industrial and Physical Pharmacy, Purdue University, 1336 Robert E Heine Pharmacy Bldg, West Lafayette, IN 47907, USA.
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D000276:Adjuvants, Immunologic / Q000737:chemistry*; D000327:Adsorption; D017607:Aluminum Compounds / Q000737:chemistry*; D000536:Aluminum Hydroxide / Q000737:chemistry*; D000818:Animals; D004355:Drug Stability; D004356:Drug Storage; D004527:Egg Proteins / Q000737:chemistry / Q000276:immunology; D008196:Lymph / Q000737:chemistry*; D009113:Muramidase / Q000737:chemistry* / Q000276:immunology; D010047:Ovalbumin / Q000737:chemistry* / Q000276:immunology; D010710:Phosphates / Q000737:chemistry*; D012756:Sheep
D000276:Adjuvants, Immunologic; D017607:Aluminum Compounds; D004527:Egg Proteins; D010710:Phosphates; D000536:Aluminum Hydroxide; D010047:Ovalbumin; D009113:Muramidase; C012714:aluminum phosphate