Pharmaceutical-induced cell apoptosis characterized by capillary zone electrophoresis.
Capillary zone electrophoresis (CZE) with a non-gel-sieving system was employed to characterize actinomycin D-induced cell apoptosis by measuring cellular DNA damage, termed DNA ladder, which proved to be thoroughly different from the DNA damage pattern of cell necrosis. The results by CZE analyses were identical to those obtained by conventional slab gel electrophoresis, demonstrating that CZE would be a reliable and more convenient technique for the identification of cell death with advantages of higher performance, high-speed, minute sample requirement, and advanced automation.
Liu
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Key Lab of MOE for Plant Developmental Biology, Wuhan University, Wuhan, 430072 People's Republic of China.
Zhang
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Liu
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Lu
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D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002460:Cell Line; D004249:DNA Damage / Q000187:drug effects; D053938:DNA Fragmentation / Q000187:drug effects; D003609:Dactinomycin / Q000494:pharmacology; D004587:Electrophoresis, Agar Gel; D019075:Electrophoresis, Capillary / Q000379:methods*; D007313:Insecta / Q000166:cytology; D009336:Necrosis
D003609:Dactinomycin