Signal transduction pathways involved in low intensity He-Ne laser-induced respiratory burst in bovine neutrophils: a potential mechanism of low intensity laser biostimulation.
OBJECTIVE
Low intensity He-Ne laser irradiation has been reported to induce respiratory burst of neutrophils for a long time, but the mechanism remains obscure. We speculated that it is mediated by some signal transduction pathways.


METHODS
The protein tyrosine kinases (PTKs) inhibitor, genistein, the phospholipase C (PLC) inhibitor, U-73122, and the protein kinase C (PKC) inhibitor, calphostin C, were used to probe signal transduction pathways of respiratory burst of bovine neutrophils which were induced by He-Ne laser at a dose of 300 J/m(2), respectively.


RESULTS
The inhibitor of PTKs can completely inhibit the He-Ne laser-induced respiratory burst of neutrophils. PLC and PKC inhibitors can obviously reduce it, but not fully inhibit it.


CONCLUSIONS
These results suggest that PTKs play a key role in the He-Ne laser-induced respiratory burst of neutrophils and [PTK-PLC-PKC-NADPH oxidase] signal transduction pathways may be involved in this process.
Duan
R
R
Laboratory of Light Transmission Optics, South China Normal University, Guangzhou, GD 510631, China.
Liu
T C
TC
Li
Y
Y
Guo
H
H
Yao
L B
LB
D000818:Animals; D002417:Cattle; D007834:Lasers; D009504:Neutrophils / Q000378:metabolism*; D016897:Respiratory Burst; D015398:Signal Transduction