title: Two new point mutations at A2062 associated with resistance to 16-membered macrolide antibiotics in mutant strains of Mycoplasma hominis.
abstract: We describe two mutants of Mycoplasma hominis PG-21 which show resistance to 16-membered macrolides but susceptibility to lincosamides, obtained by in vitro exposure to increasing doses of josamycin. The 23S rRNA gene showed that each had a mutation (A2062G and A2062T) corresponding to nucleotide 2062 in Escherichia coli, which was associated with the acquired phenotype.
authors.affiliation: Dipartimento di Scienze Microbiologiche e Scienze Ginecologiche, Università degli Studi, Via Androne 81, 95124 Catania, Italy. furneri@mbox.unict.it
authors.forename: P M
authors.initials: PM
authors.lastname: Furneri
authors.forename: G
authors.initials: G
authors.lastname: Rappazzo
authors.forename: M P
authors.initials: MP
authors.lastname: Musumarra
authors.forename: P
authors.initials: P
authors.lastname: Di Pietro
authors.forename: L S
authors.initials: LS
authors.lastname: Catania
authors.forename: L S
authors.initials: LS
authors.lastname: Roccasalva
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001483:Base Sequence; D004269:DNA, Bacterial / Q000032:analysis; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D018942:Macrolides; D008969:Molecular Sequence Data; D019535:Mycoplasma hominis / Q000187:drug effects / Q000235:genetics*; D010641:Phenotype; D017354:Point Mutation; D012338:RNA, Ribosomal, 23S / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D018942:Macrolides; D012338:RNA, Ribosomal, 23S