Advances in directed protein evolution by recursive genetic recombination: applications to therapeutic proteins.
Recent developments in directed evolution technologies combined with innovations in robotics and screening methods have revolutionized protein engineering. These methods are being applied broadly to many fields of biotechnology, including chemical engineering, agriculture and human therapeutics. More specifically, DNA shuffling and other methods of genetic recombination and mutation have resulted in the improvement of proteins of therapeutic interest. Optimizing genetic diversity and fitness through iterative directed evolution will accelerate improvements in engineered protein therapeutics.
Kurtzman
A L
AL
Maxygen Inc., 515 Galveston Drive, Redwood City, CA 94063, USA. aaron.kurtzman@maxygen.com
Govindarajan
S
S
Vahle
K
K
Jones
J T
JT
Heinrichs
V
V
Patten
P A
PA
D000906:Antibodies / Q000235:genetics / Q000627:therapeutic use; D016207:Cytokines / Q000235:genetics / Q000627:therapeutic use; D019020:Directed Molecular Evolution / Q000379:methods*; D014644:Genetic Variation; D016296:Mutagenesis / Q000235:genetics; D015202:Protein Engineering / Q000379:methods*; D011506:Proteins / Q000235:genetics* / Q000627:therapeutic use*; D011995:Recombination, Genetic / Q000235:genetics*
D000906:Antibodies; D016207:Cytokines; D011506:Proteins