UV-excited chlorophyll fluorescence as a tool for the assessment of UV-protection by the epidermis of plants.
Recently, a new method for estimating epidermal transmission of UV radiation in higher plants has been proposed. The empirical evidence for the usefulness of this method is reviewed here. Direct comparison with spectroscopically determined epidermal transmission yielded equivalent results. A linear correlation to the concentration of epidermal screening compounds has been shown. Relating UV-A and UV-B absorbance allowed some preliminary conclusions about the chemical nature of the screening compounds. A new portable apparatus is presented for the first time, which allows the non-destructive assessment of UV-A screening even under field conditions. Repeated measurements on identical leaves over a time-course of 6 d demonstrated a strong age-dependence in the capacity for the synthesis of UV-A screening compounds upon exposure to UV-B radiation. It is concluded that the new method may provide a valuable tool for the investigation of the acclimation of plants to UV-B radiation and, when accompanied by HPLC analysis, of the reaction of phenolic metabolism to environmental stimuli.
Bilger
W
W
Department of Biology and Nature Conservation, Agricultural University of Norway, PO Box 5014, N-1432 As, Norway.
Johnsen
T
T
Schreiber
U
U
D000064:Acclimatization / Q000502:physiology; D001901:Botany / Q000379:methods*; D002734:Chlorophyll / Q000528:radiation effects*; D007887:Fabaceae; D005419:Flavonoids; D005453:Fluorescence; D005470:Fluorometry / Q000379:methods*; D019441:Plant Epidermis / Q000378:metabolism / Q000528:radiation effects; D018515:Plant Leaves / Q000528:radiation effects; D014466:Ultraviolet Rays
D005419:Flavonoids; D002734:Chlorophyll