Effects of phenobarbital on activity of mitochondrial enzymes in peripheral blood lymphocytes and oxidative phosphorylation in liver mitochondria.
Treatment with phenobarbital (30 mg/kg) for 2 weeks increased succinate dehydrogenase activity in peripheral blood lymphocytes of male rats. In 38% phenobarbital-treated rats succinate-cytochrome c oxidoreductase activity was lower than in the control due to accumulation of cells exhibiting no enzyme activity; in 44% animals this parameter was higher than in the control. The rates of state 3 respiration (oxidation substrate succinate), phosphorylation, and uncoupled respiration in liver mitochondria (oxidation substrate glutamate/malate mixture) increased after 35-day treatment with phenobarbital. The respiratory control and ADP/O ratio for these substrates did not differ from the control.
Komissarova
I A
IA
Biotiki Medical Research-and-Production Complex, Moscow.
Nartsissov
Y R
YR
Burbenskaya
N M
NM
D000818:Animals; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology*; D008214:Lymphocytes / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D008928:Mitochondria / Q000187:drug effects* / Q000201:enzymology; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000378:metabolism; D010085:Oxidative Phosphorylation / Q000187:drug effects*; D010634:Phenobarbital / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013384:Succinate Cytochrome c Oxidoreductase / Q000378:metabolism*; D013385:Succinate Dehydrogenase / Q000378:metabolism*; D013997:Time Factors
D018691:Excitatory Amino Acid Antagonists; D013384:Succinate Cytochrome c Oxidoreductase; D013385:Succinate Dehydrogenase; D010634:Phenobarbital