Arabinogalactan protein and wall-associated kinase in a plasmalemmal reticulum with specialized vertices.
Arabinogalactan protein and wall-associated kinase (WAK) are suspected to be regulatory players at the interface between cytoplasm and cell wall. Both WAK(s) and arabinogalactan shown likely to represent arabinogalactan protein(s) have been visualized there with computational optical-sectioning microscopy. The arabinogalactan occurs in a polyhedral array at the external face of the cell membrane. WAK, and other proteins as yet unidentified, appear to fasten the membrane to the wall at vertices of the array. Evidence is presented that the array bears an important part of the mechanical stress experienced by the membrane, and it is speculated that the architectural organization of arabinogalactan protein, WAK, and other components of the array is critical for coordination of endomembrane activities, growth, and differentiation. The array has been named the plasmalemmal reticulum.
Gens
J S
JS
The Gladys Levis Allen Laboratory of Plant Sensory Physiology, Biology Department, Washington University, St. Louis, Missouri, USA.
Fujiki
M
M
Pickard
B G
BG
D000911:Antibodies, Monoclonal; D029681:Arabidopsis Proteins; D002462:Cell Membrane / Q000201:enzymology / Q000378:metabolism / Q000502:physiology; D002468:Cell Physiological Phenomena; D002473:Cell Wall / Q000737:chemistry / Q000201:enzymology / Q000378:metabolism / Q000502:physiology*; D003593:Cytoplasm / Q000737:chemistry / Q000201:enzymology / Q000378:metabolism / Q000502:physiology; D004721:Endoplasmic Reticulum / Q000502:physiology; D005455:Fluorescent Antibody Technique; D005685:Galactans / Q000032:analysis / Q000378:metabolism*; D008565:Membrane Proteins / Q000032:analysis / Q000378:metabolism*; D010940:Plant Proteins / Q000032:analysis / Q000378:metabolism*; D010947:Plants, Toxic; D011494:Protein Kinases / Q000032:analysis / Q000378:metabolism*; D014026:Nicotiana / Q000737:chemistry / Q000166:cytology* / Q000201:enzymology / Q000378:metabolism
D000911:Antibodies, Monoclonal; D029681:Arabidopsis Proteins; D005685:Galactans; D008565:Membrane Proteins; D010940:Plant Proteins; D011494:Protein Kinases; C101093:WAK1 protein, Arabidopsis; C005653:arabinogalactan