Control of reaction chemoselectivity with a circular DNA template in the ligation of short oligodeoxyribonucleotides.
A study has been conducted to examine the chemoselective control attained in the ligation of short oligodeoxyribonucleotides (ODNs) directed by association on a circular DNA template through triplex formation relative to the same ligation on a single-strand DNA template through duplex formation. The highly efficient, chemospecific ligation achieved under a variety of conditions on the circular DNA template presumably arises from the higher substrate-template association resulting in improved transition state accessibility and protection of the ODNs from side reactions.
Selvasekaran
J
J
American Radiolabeled Corporation, St. Louis, MO 63146, USA.
Turnbull
K D
KD
D002234:Carbodiimides / Q000737:chemistry; D004247:DNA; D004270:DNA, Circular / Q000378:metabolism*; D004277:DNA, Single-Stranded / Q000138:chemical synthesis; D006860:Hydrogen Bonding; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D013698:Templates, Genetic
D002234:Carbodiimides; D004270:DNA, Circular; D004277:DNA, Single-Stranded; D009838:Oligodeoxyribonucleotides; C051802:triplex DNA; D004247:DNA