title: [Specific characteristics of composition of synthetic nutrient media for cultivation of Hemophilus influenzae type b from the standpoint of bacterial metabolism].
abstract: In many countries vaccination against Haemophilus influenzae of type b (Hib) has permitted the liquidation of severe generalized forms of infections caused by these bacteria. The vaccine is obtained on the basis of Hib capsular polysaccharide. To obtain pure capsular polysaccharide, Hib should be cultivated on synthetic nutrient media. The present review deals with the data substantiating the advantages of using synthetic nutrient media for the cultivation of these bacteria with a view to obtaining pure capsular polysaccharide.
authors.affiliation: Mechnikov Research Institute for Vaccines and Sera, Moscow, Russia.
authors.forename: O E
authors.initials: OE
authors.lastname: Orlova
authors.forename: N E
authors.initials: NE
authors.lastname: Iastrebova
authors.forename: S I
authors.initials: SI
authors.lastname: Elkina
mesh_terms: D016667:Bacterial Capsules; D002648:Child; D003470:Culture Media; D018073:Haemophilus Vaccines / Q000096:biosynthesis / Q000302:isolation & purification; D019741:Haemophilus influenzae type b / Q000254:growth & development* / Q000378:metabolism*; D006801:Humans; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis / Q000302:isolation & purification
chemical_list: D003470:Culture Media; D018073:Haemophilus Vaccines; C044237:Haemophilus influenzae type b polysaccharide vaccine; D011135:Polysaccharides, Bacterial