title: Modification of a commercial electrospray nebulizer for operation in a liquid chromatography/mass spectrometry system at flow rates in the low microL/min range.
abstract: A simple and inexpensive approach to convert the electrospray nebulizer of a commercial liquid chromatography/mass spectrometry (LC/MS) system (HP 1100) to accommodate lower flow rates has been proposed and evaluated. This modification consists of simply replacing the nebulizer needle by a commercially available stainless steel needle with a smaller internal diameter. Experiments were conducted in order to optimize operational parameters. Using two different internal diameter needle sizes, flow rates ranging from 1 to 250 microL/min could be accommodated. The modification presented allows an extension of the range of compatible flow rates without major modification of the standard design of the interface.
authors.affiliation: RTP Pharma Inc., 1000 Chemin du Golf, Ile des Soeurs, Québec, Canada, H3E 1H4. acarrier@rtp-pharma.com
authors.forename: A
authors.initials: A
authors.lastname: Carrier
authors.forename: J
authors.initials: J
authors.lastname: Parent
mesh_terms: D002851:Chromatography, High Pressure Liquid; D016572:Cyclosporine / Q000032:analysis; D004340:Drug Contamination; D007166:Immunosuppressive Agents / Q000032:analysis; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation* / Q000379:methods
chemical_list: D007166:Immunosuppressive Agents; D016572:Cyclosporine