A new "native ligation" procedure for peptide-oligonucleotide conjugation.
"Native ligation", a powerful method of joining peptide fragments, has been applied successfully to peptide-oligonucleotide conjugation. Novel reagents are described for the solid-phase synthesis of peptide N-terminal thioesters and 5'-cysteinyl oligonucleotides suitable for ligation reactions.
Stetsenko
D A
DA
Medical Research Council, Laboratory of Molecular Biology, Hills Road, CB2 2QH Cambridge, England.
Gait
M J
MJ
D003545:Cysteine / Q000031:analogs & derivatives*; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry
D009841:Oligonucleotides; D010455:Peptides; D003545:Cysteine