The growth of Paracoccus halodenitrificans in a defined medium.
A synthetic medium, consisting of inorganic salts and any of a number of carbon sources, supported the aerobic growth of Paracoccus halodenitrificans when supplemented with thiamine.  The same medium plus an appropriate nitrogenous oxide supported anaerobic growth when additionally supplemented with methionine.  The observation that vitamin B12 or betaine replaced methionine suggested that P. halodenitrificans had a defect in the cobalamin-dependent pathway for methionine biosynthesis, as well as the inability to synthesize betaine when growing anaerobically.
Hochstein
L I
LI
Extraterrestrial Research Division, National Aeronautics and Space Administration, Ames Research Center, Moffett Field, CA 94035, USA.
Tomlinson
G A
GA
D000645:Ammonium Sulfate / Q000032:analysis / Q000378:metabolism; D001622:Betaine / Q000032:analysis / Q000378:metabolism*; D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000032:analysis; D003470:Culture Media / Q000737:chemistry; D005296:Ferrous Compounds / Q000032:analysis / Q000378:metabolism; D008278:Magnesium Sulfate / Q000032:analysis / Q000378:metabolism; D008715:Methionine / Q000032:analysis / Q000378:metabolism*; D009566:Nitrates / Q000032:analysis / Q000378:metabolism; D010230:Paracoccus / Q000254:growth & development* / Q000378:metabolism*; D010710:Phosphates / Q000032:analysis / Q000378:metabolism; D017680:Potassium Compounds / Q000032:analysis / Q000378:metabolism; D012965:Sodium Chloride / Q000032:analysis / Q000378:metabolism; D019354:Sodium Lactate / Q000032:analysis / Q000378:metabolism; D013831:Thiamine / Q000032:analysis / Q000378:metabolism*; D014805:Vitamin B 12 / Q000032:analysis / Q000378:metabolism*
D002241:Carbohydrates; D003470:Culture Media; D005296:Ferrous Compounds; D009566:Nitrates; D010710:Phosphates; D017680:Potassium Compounds; C020748:ferrous sulfate; D001622:Betaine; D012965:Sodium Chloride; D008278:Magnesium Sulfate; D008715:Methionine; C013216:potassium phosphate; D014805:Vitamin B 12; C023844:potassium nitrate; D000645:Ammonium Sulfate; D019354:Sodium Lactate; D013831:Thiamine