title: Synthesis and study of the fluorescein conjugate of the nucleotide dPTP.
abstract: The synthesis of a fluorescein conjugate on the non-natural base P is described. The ability of the newly synthesised fluorescein--dPTP and of a fluorescein-11-dUTP to compete with the natural nucleotide TTP was also studied. Overall the efficiency of labelling a nucleic acid with a fluorescein moiety was found to be approximately equal.
authors.affiliation: Amersham Pharmacia Biotech UK Limited, Amersham Place, Little Chalfont, Buckinghamshire HP7 9NA, England.
authors.forename: W J
authors.initials: WJ
authors.lastname: Cummins
authors.forename: A L
authors.initials: AL
authors.lastname: Hamilton
authors.forename: C L
authors.initials: CL
authors.lastname: Smith
authors.forename: M S
authors.initials: MS
authors.lastname: Briggs
mesh_terms: D001667:Binding, Competitive; D004256:DNA Polymerase I / Q000378:metabolism; D003856:Deoxyuracil Nucleotides / Q000378:metabolism; D005452:Fluoresceins / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D009711:Nucleotides / Q000138:chemical synthesis* / Q000737:chemistry* / Q000378:metabolism; D010078:Oxazines / Q000737:chemistry* / Q000378:metabolism; D011721:Pyrenes / Q000138:chemical synthesis*; D011743:Pyrimidines / Q000737:chemistry* / Q000378:metabolism
chemical_list: D003856:Deoxyuracil Nucleotides; D005452:Fluoresceins; D009711:Nucleotides; D010078:Oxazines; D011721:Pyrenes; D011743:Pyrimidines; C119745:pyrene deoxynucleotide; C062254:6H,8H-3,4-dihydropyrimido(4,5-c)(1,2)oxazin-7-one; C069632:fluorescein-11-deoxyuridine triphosphate; D004256:DNA Polymerase I