Changes in the distribution of anionic constituents in secretory granules of mouse pancreatic acinar cells after pilocarpine-induced degranulation.
We used cationized colloidal gold (CCG) to investigate the distribution of anionic sites in different secretory granules of mouse pancreatic acinar cell regranulation. Localization of anionic sites with CCG was carried out on ultrathin sections of a mouse pancreas, fixed in Karnovsky's fixative and OsO4 and embedded in Araldite. After pilocarpine-stimulated degranulation, there was a marked diminution in the anionic charge density of immature and mature granules of the 4-hr group (approximately 43.0 gold particles/microm2) compared to the 8-hr mature granules group (approximately 64.6 gold particles/microm2). Scattergram analysis to investigate the correlation between section profile size and cationized gold labeling density revealed a reverse correlation, the small granule profiles demonstrated a higher density compared to the larger profiles of the same group. On the basis of these observations, it appears that a post-translational processing of secretory content influences the granule anionic charge and thus may affect the intragranular buffer capacity.
Shoichetman
T
T
Department of Pathology, Sackler School of Medicine, Tel Aviv University, Ramat Aviv, Israel.
Skutelsky
E
E
Lew
S
S
Hammel
I
I
D000818:Animals; D000838:Anions; D015550:Cell Degranulation; D005260:Female; D017979:Gold Colloid; D006651:Histocytochemistry; D051379:Mice; D008813:Mice, Inbred ICR; D008854:Microscopy, Electron; D010179:Pancreas / Q000737:chemistry* / Q000166:cytology / Q000648:ultrastructure; D010862:Pilocarpine; D011509:Proteoglycans / Q000737:chemistry; D022142:Secretory Vesicles / Q000737:chemistry* / Q000648:ultrastructure
D000838:Anions; D017979:Gold Colloid; D011509:Proteoglycans; D010862:Pilocarpine