3'-Terminal sequence analysis of the RNA genome of the Indian isolate of cardamom mosaic virus: a new member of genus Macluravirus of potyviridae.
Cardamom mosaic virus, a possible member of the family Potyviridae has been associated with the mosaic disease (Katte disease) of small cardamom in India. A virus isolated from the symptomatic cardamom leaves was positive in ELISA only with antiserum to the Guatemalan isolate of cardamom mosaic virus and not with a number of other potyviruses. The size of the viral RNA (8.5 kb) and the molecular weight of the coat protein (CP) (38 kDa) were determined. A 1.8-kb product containing the partial nuclear inclusion body (NIb) gene, the entire coat protein gene and the 3' untranslated region (UTR) was amplified by reverse transcription (RT) and polymerase chain reaction (PCR), cloned and sequenced. The viral origin of the clone was confirmed by Northern hybridization with viral RNA. The experimentally determined N-terminal sequence of the CP matched with the deduced amino acid sequence of the CP gene. Sequence analysis of the clone suggests that the cardamom mosaic virus is a member of the Maclurvirus genus of the family Potyviridae.
Jacob
T
T
Department of Plant Biotechnology, School of Biotechnology, Madurai Kamaraj University, India.
Usha
R
R
D020413:3' Untranslated Regions; D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites; D002213:Capsid / Q000032:analysis / Q000235:genetics; D012321:DNA-Directed RNA Polymerases; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D028523:Elettaria / Q000821:virology*; D010450:Endopeptidases / Q000378:metabolism; D016679:Genome, Viral; D007194:India; D008969:Molecular Sequence Data; D010802:Phylogeny; D017800:Potyvirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D012367:RNA, Viral; D017423:Sequence Analysis, RNA; D017386:Sequence Homology, Amino Acid; D012967:Sodium Dodecyl Sulfate; D014764:Viral Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism
D020413:3' Untranslated Regions; D012367:RNA, Viral; D014764:Viral Proteins; D012967:Sodium Dodecyl Sulfate; C061396:nuclear inclusion protein b, mosaic viruses; D012321:DNA-Directed RNA Polymerases; D010450:Endopeptidases