Efficient applications of capillary electrophoresis-tandem mass spectrometry to the analysis of adrenoreceptor antagonist enantiomers using a partial filling technique.
Throughout the separation of chiral basic drugs by capillary electrophoresis (CE) with neutral hydroxypropyl-beta-cyclodextrin (HP-beta-CD) as chiral selector, the sensitivity of detection can be improved by using tandem mass spectrometric (MS-MS) detection with a partial filling technique rather than with UV spectrometric detection. Prior to sample injection. the capillary was partly filled with HP-beta-CD dissolved in volatile ammonium formate buffer (pH 4, ionic strength 50 mM). The effects of modifying the HP-beta-CD concentration in the selector zone and the length of the separation zone on the enantioresolution and the signal-to-noise ratio of the pseudo-molecular MH+ ion were investigated. For a given selector zone length, as the concentration of the neutral cyclodextrin increases, the resolution between enantiomers becomes higher (the opposite of the behavior of the signal-to-noise ratio) and then reaches an optimum value. The decrease of the selector zone length lowered the resolution between the enantiomers but increased peak efficiencies and signal-to-noise ratio values. Accordingly, partial capillary filling at 80% (v/v) and 10 mM concentration of HP-beta-CD was selected as a suitable compromise between resolution and sensitivity of MS detection. Limits of detection for each adrenoreceptor antagonist enantiomer were 5 ng/ml (0.02 microM) in CE-MS-MS instead of 150 ng/ml (0.60 microM) in CE-UV, which enhances sensitivity by a factor of 30.
Grard
S
S
Institut de Chimie Organique et Analytique, CNRS UMR 6005, Université d'Orléans, France.
Morin
P H
PH
Dreux
M
M
Ribet
J P
JP
D018674:Adrenergic Antagonists / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D012680:Sensitivity and Specificity; D013237:Stereoisomerism
D018674:Adrenergic Antagonists