title: Transformation of 2,2'-dichlorodiisopropyl ether in mixed and pure culture.
abstract: An aerobic enrichment culture derived from a groundwater contaminated with organic and chloroorganic compounds was adapted to the transformation of 2,2'-dichlorodiisopropyl ether (DDE) in a continuous fixed-bed bioreactor. Continuous DDE removal efficiencies over 90% were achieved with a model water containing 3.3 mM methanol as co-substrate at DDE loading rates of up to 150 micromol l(-1) day(-1) with a hydraulic retention time of 24 h. In batch cultures, a stoichiometric release of 2 micromol chloride per micromol DDE transformed was observed. From the mixed culture, a strain was isolated that is able to grow on DDE as the sole energy and carbon source, tolerating DDE concentrations of up to 1 mM. Based on 16S rRNA sequencing, the strain is affiliated with the genus Rhodococcus.
authors.affiliation: Institut für Technischen Umweltschutz, Fachgebiet Siedlungswasserwirtschaft, Berlin, Germany.
authors.forename: R
authors.initials: R
authors.lastname: Hauck
authors.forename: L
authors.initials: L
authors.lastname: Adrian
authors.forename: P
authors.initials: P
authors.lastname: Wendler
authors.forename: M
authors.initials: M
authors.lastname: Amidjojo
authors.forename: W
authors.initials: W
authors.lastname: Hegemann
authors.forename: H
authors.initials: H
authors.lastname: Görisch
mesh_terms: D000332:Aerobiosis; D001673:Biodegradation, Environmental; D019149:Bioreactors; D002712:Chlorides / Q000378:metabolism; D003470:Culture Media; D017753:Ecosystem; D005019:Ethyl Ethers / Q000378:metabolism*; D005618:Fresh Water / Q000382:microbiology*; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012240:Rhodococcus / Q000145:classification / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D017422:Sequence Analysis, DNA; D014877:Water Pollution, Chemical
chemical_list: D002712:Chlorides; D003470:Culture Media; D005019:Ethyl Ethers; D012336:RNA, Ribosomal, 16S; C052560:bis(2-chloro-1-methylethyl) ether