title: In vitro fertilizing capacity of deep frozen boar semen packaged in 0.5 and 5 ml straws.
abstract: The aim of this study was to evaluate the straw size effect used for freezing on the in vitro fertilizing capacity. Twenty-one ejaculates from seven fertile boars were frozen under controlled conditions in 0.5 and 5 ml straws. Thawed semen was compared to fresh semen. For fresh and thawed semen in 0.5 and 5 ml straws, the results were: 92.18, 77.38 and 79.04% sperm penetration; 80.68, 66.89 and 69.33% monospermy; 11.51, 10.49 and 9.74% polyspermy; 86.19, 47.14 and 47.02% motility and 75.52, 48.19 and 46.81% normal apical ridge (NAR), respectively. Analysis of variance and test of multiple comparisons showed that under the conditions employed, penetration, monospermy, motility and NAR were significantly reduced by freezing-thawing, but polyspermy was much less affected. The results obtained suggest that frozen boar semen is adequate for in vitro fertilization. In addition freezing in 5 ml straws did not have any detrimental effect on either penetration, monospermy, polyspermy, motility and NAR, in comparison with freezing in 0.5 ml straws.
authors.affiliation: Facultad de Veterinaria, Universidad Complutense de Madrid, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Córdova
authors.forename: J F
authors.initials: JF
authors.lastname: Pérez
authors.forename: B
authors.initials: B
authors.lastname: Lleó
authors.forename: C
authors.initials: C
authors.lastname: García Artiga
authors.forename: S
authors.initials: S
authors.lastname: Martín Rillo
mesh_terms: D000818:Animals; D015925:Cryopreservation / Q000662:veterinary*; D005306:Fertilization / Q000502:physiology*; D005307:Fertilization in Vitro / Q000662:veterinary; D008297:Male; D008858:Microscopy, Phase-Contrast / Q000662:veterinary; D012661:Semen / Q000502:physiology*; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D013081:Sperm Motility; D013084:Sperm-Ovum Interactions; D013552:Swine / Q000502:physiology*