A plant Shaker-like K+ channel switches between two distinct gating modes resulting in either inward-rectifying or "leak" current.
Among the Shaker-like plant potassium channels, AKT2 is remarkable because it mediates both instantaneous "leak-like" and time-dependent hyperpolarisation-activated currents. This unique gating behaviour has been analysed in Xenopus oocytes and in COS and Chinese hamster ovary cells. Whole-cell and single-channel data show that (i) AKT2 channels display two distinct gating modes, (ii) the gating of a given AKT2 channel can change from one mode to the other and (iii) this conversion is under the control of post-translational factor(s). This behaviour is strongly reminiscent of that of the KCNK2 channel, recently reported to be controlled by its phosphorylation state.
Dreyer
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I
Laboratoire de Biochimie et Physiologie Moléculaire des Plantes, UMR 5004, Agro-M/CNRS/INRA/UM2, Place Viala, 34060 Montpellier Cedex 1, France.
Michard
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E
Lacombe
B
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Thibaud
J B
JB
D000818:Animals; D029681:Arabidopsis Proteins; D016466:CHO Cells; D019556:COS Cells; D003001:Cloning, Molecular; D006224:Cricetinae; D018076:DNA, Complementary / Q000378:metabolism; D004594:Electrophysiology; D009865:Oocytes / Q000378:metabolism; D010766:Phosphorylation; D010940:Plant Proteins / Q000737:chemistry* / Q000378:metabolism*; D011188:Potassium / Q000378:metabolism; D015221:Potassium Channels / Q000737:chemistry* / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D051637:Shaker Superfamily of Potassium Channels; D014981:Xenopus
C097485:AKT2 protein, Arabidopsis; D029681:Arabidopsis Proteins; D018076:DNA, Complementary; D010940:Plant Proteins; D015221:Potassium Channels; D051637:Shaker Superfamily of Potassium Channels; D011188:Potassium