Characterization of a novel human cell-cycle-regulated homologue of Drosophila dlg1.
Cell cycle defects have been associated with the process of carcinogenesis in many studies. Here we report the cloning and analysis of the novel gene KIAA0008 (GenBank acc. no. D13633). Chromosomal localization experiments assigned the gene to chromosome 14q22-q23. The mRNA transcript was found to be cell cycle regulated, expressed at S-phase, and maintained at both G2-and M-phases. In situ hybridization showed expression in proliferating colon and breast (tumor) tissues. Structurally, KIAA0008 shares homology with the Drosophila melanogaster discs large-1 (dlg1) tumor suppressor gene and membrane-associated guanylate kinase protein family members. The potential involvement of KIAA0008 in cell proliferation is discussed, along with its sequence identity and tissue distribution.
Bassal
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S
Division of Radiation Oncology, Peter MacCallum Cancer Institute, Locked Bag 1, A'Beckett St., Melbourne, Victoria, 8006, Australia.
Nomura
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N
Venter
D
D
Brand
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K
McKay
M J
MJ
van der Spek
P J
PJ
D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D015152:Blotting, Northern; D001943:Breast Neoplasms / Q000235:genetics; D002453:Cell Cycle / Q000502:physiology*; D002874:Chromosome Mapping; D002883:Chromosomes, Human, Pair 14 / Q000235:genetics; D003110:Colonic Neoplasms / Q000235:genetics; D000074288:Discs Large Homolog 1 Protein; D004331:Drosophila melanogaster / Q000235:genetics; D005260:Female; D005786:Gene Expression Regulation; D015972:Gene Expression Regulation, Neoplastic; D016147:Genes, Tumor Suppressor / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D008565:Membrane Proteins; D009363:Neoplasm Proteins; D011506:Proteins / Q000235:genetics*; D012313:RNA / Q000235:genetics / Q000378:metabolism; D014018:Tissue Distribution
D048868:Adaptor Proteins, Signal Transducing; C513314:DLG1 protein, human; C465041:DLGAP5 protein, human; D000074288:Discs Large Homolog 1 Protein; D008565:Membrane Proteins; D009363:Neoplasm Proteins; D011506:Proteins; D012313:RNA