title: Influence of altered gravity on brain cellular energy and plasma membrane metabolism of developing lower aquatic vertebrates.
abstract: Biochemical analyses of the brain of cichlid fish larvae, exposed for 7 days to increased acceleration of 3g (hyper-g), revealed an increase in energy availability (succinate dehydrogenase activity, SDH), and in mitochondrial energy transformation (creatine kinase, Mia-CK), but no changes in an energy consumptive process (high-affinity Ca(2+)-ATPase).  Brain glucose-6-phosphate dehydrogenase (G6PDH) of developing fish was previously found to be increased after hyper-g exposure.  Three respectively 5 hours thereafter dramatic fluctuations in enzyme activity were registered.  Analysing the cytosolic or plasma membrane-located brain creatine kinase (BB-CK) of clawed toad larvae after long-term hyper-g exposure a significant increase in enzyme activity was demonstrated, whereas the activity of a high affinity Ca(2+)-ATPase remained unaffected.
authors.affiliation: University of Stuttgart-Hohenheim, Institute of Zoology, Stuttgart, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Slenzka
authors.forename: R
authors.initials: R
authors.lastname: Appel
authors.forename: T h
authors.initials: Th
authors.lastname: Kappel
authors.forename: H
authors.initials: H
authors.lastname: Rahmann
mesh_terms: D000818:Animals; D001921:Brain / Q000201:enzymology*; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D002462:Cell Membrane / Q000201:enzymology; D003402:Creatine Kinase / Q000378:metabolism*; D004734:Energy Metabolism / Q000502:physiology*; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D018471:Hypergravity; D007814:Larva / Q000201:enzymology; D008928:Mitochondria / Q000201:enzymology; D010471:Perches / Q000254:growth & development; D013385:Succinate Dehydrogenase / Q000378:metabolism*; D014982:Xenopus laevis / Q000254:growth & development
chemical_list: D005954:Glucosephosphate Dehydrogenase; D013385:Succinate Dehydrogenase; D003402:Creatine Kinase; D000252:Calcium-Transporting ATPases