A novel multilocus genotyping assay to identify genetic predictors of stroke in sickle cell anaemia.
We describe a novel multilocus genotyping assay permitting simultaneous identification of 60 candidate markers for stroke in sickle cell anaemia (SCA). Based on cerebral magnetic resonance imaging (MRI), 69 patients were divided into stroke and control groups. The variant allele, CBS 278thr, showed protection from stroke, whereas the apoE3 allele showed a trend towards association with increased stroke risk. Several other variant alleles [TNFalpha (-308)A, CETP (-628)A, apoCIII (-641)A] showed a trend towards significant associations with stroke risk. These preliminary results on a small group of patients suggest that a multilocus genotyping assay may be valuable in identifying genes that increase the risk of stroke in SCA.
Hoppe
C
C
Department of Hematology/Oncology, Children's Hospital Oakland, Oakland, CA 94609, USA. choppe@mail.cho.org
Cheng
S
S
Grow
M
M
Silbergleit
A
A
Klitz
W
W
Trachtenberg
E
E
Erlich
H
H
Vichinsky
E
E
Styles
L
L
D000293:Adolescent; D000483:Alleles; D000755:Anemia, Sickle Cell / Q000150:complications / Q000235:genetics*; D001057:Apolipoproteins E / Q000235:genetics; D016022:Case-Control Studies; D002648:Child; D003541:Cystathionine beta-Synthase / Q000235:genetics*; D005819:Genetic Markers; D005838:Genotype; D006801:Humans; D016017:Odds Ratio; D016133:Polymerase Chain Reaction / Q000379:methods; D020521:Stroke / Q000209:etiology / Q000235:genetics*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
D001057:Apolipoproteins E; D005819:Genetic Markers; D014409:Tumor Necrosis Factor-alpha; D003541:Cystathionine beta-Synthase