title: Changes in ascorbic acid content in primary cultured rat hepatocytes exposed to 2,2'-azobis (2-amidinopropane) dihydrochloride, a radical initiator.
abstract: Changes in ascorbate content in primary cultured rat hepatocytes exposed to oxidative stress derived from water soluble radical initiator 2,2'-azobis (2-amidinopropane) dihydrochloride (AAPH) were examined. Cells were exposed to 0.05 and 5 mg/ml of AAPH as 'mild' and severe' oxidative stresses, respectively. Lipid peroxidation in hepatocytes was induced by 'severe' oxidative stress, but not by 'mild' oxidative stress. Ascorbate decreased at 6 hr after administration of both mild' and severe' oxidative stresses, and recovered to the control level after a further 6 hr. In cells treated with 'severe oxidative stress, however, total ascorbate (reduced form plus oxidized form) had increased 24 hr after administration. These results indicated that consumption alone did not account for the increase of ascorbate in hepatocytes under oxidative stress.
authors.affiliation: Department of Animal Products, National Institute of Livestock and Grassland Science, Ibaraki, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Sasakii
authors.forename: K
authors.initials: K
authors.lastname: Kimura
authors.forename: Y
authors.initials: Y
authors.lastname: Kitaguchi
authors.forename: T
authors.initials: T
authors.lastname: Onoue
authors.forename: H
authors.initials: H
authors.lastname: Ogura
authors.forename: Y
authors.initials: Y
authors.lastname: Aoyagi
mesh_terms: D000578:Amidines / Q000494:pharmacology*; D000818:Animals; D001205:Ascorbic Acid / Q000096:biosynthesis / Q000378:metabolism*; D022781:Hepatocytes / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D016877:Oxidants / Q000494:pharmacology*; D018384:Oxidative Stress / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D000578:Amidines; D016877:Oxidants; D017392:Thiobarbituric Acid Reactive Substances; C046728:2,2'-azobis(2-amidinopropane); D001205:Ascorbic Acid