title: The effect of osmotic stress on the production of nukacin ISK-1 from Staphylococcus warneri ISK-1.
abstract: The effects of several additives on the production of a lantibiotic, nukacin ISK-1, from Staphylococcus warneri ISK-1, in batch fermentation were studied. NaCl, KCl and sorbitol stimulated nukacin ISK-1 production. The addition of 1.4 M NaCl increased nukacin ISK-1 activity 1.5-fold over the control, while cell growth and glucose consumption were inhibited. Nukacin ISK-1 production increased with increasing osmolarity of the medium up to about 3 osmol/kg; however, further increases in osmolarity diminished productivity, irrespective of the kind of additive. Northern blot analysis showed that transcription of the nukacin ISK-1 structural gene (nukA) was activated in the presence of 1.4 M NaC1. These data indicate that the stimulation effect was due to osmotic stress, which acted, at least in part, at the transcriptional level on the nukA gene.
authors.affiliation: Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Fukuoka, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Sashihara
authors.forename: M
authors.initials: M
authors.lastname: Dan
authors.forename: H
authors.initials: H
authors.lastname: Kimura
authors.forename: H
authors.initials: H
authors.lastname: Matsusaki
authors.forename: K
authors.initials: K
authors.lastname: Sonomoto
authors.forename: A
authors.initials: A
authors.lastname: Ishizaki
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001430:Bacteriocins / Q000096:biosynthesis* / Q000235:genetics; D001622:Betaine / Q000494:pharmacology; D015964:Gene Expression Regulation, Bacterial; D009994:Osmolar Concentration; D009997:Osmotic Pressure; D012965:Sodium Chloride / Q000494:pharmacology*; D013012:Sorbitol / Q000494:pharmacology; D013210:Staphylococcus / Q000187:drug effects / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D001430:Bacteriocins; C421186:nukacin ISK-1; D001622:Betaine; D012965:Sodium Chloride; D013012:Sorbitol