Step-by-step control by MALDI-TOF MS of an oligonucleotide synthesis on standard CPG solid-support.
MALDI-TOF mass spectrometry was used to monitor DNA solid-phase synthesis on long-chain alkylamine controlled-pore glass (LCAA-CPG) with a standard succinyl linker between the solid support and the growing oligonucleotide.
Meyer
A
A
Lab. de Chimie Organique Biomoléculaire de Synthèse, UMR 5625 CNRS-UM2, Université Montpellier II, Place E. Bataillon, 34095, Montpellier, France.
Debart
F
F
Imbach
J L
JL
Vasseur
J J
JJ
D005898:Glass / Q000737:chemistry; D009841:Oligonucleotides / Q000138:chemical synthesis*; D009943:Organophosphorus Compounds / Q000737:chemistry; D017422:Sequence Analysis, DNA / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D009841:Oligonucleotides; D009943:Organophosphorus Compounds; C434331:phosphoramidite