title: Complete nucleotide sequence of a 43-kilobase genomic island associated with the multidrug resistance region of Salmonella enterica serovar Typhimurium DT104 and its identification in phage type DT120 and serovar Agona.
abstract: This study describes the characterization of the recently described Salmonella genomic island 1 (SGI1) (D. A. Boyd, G. A. Peters, L.-K. Ng, and M. R. Mulvey, FEMS Microbiol. Lett. 189:285-291, 2000), which harbors the genes associated with the ACSSuT phenotype in a Canadian isolate of Salmonella enterica serovar Typhimurium DT104. A 43-kb region has been completely sequenced and found to contain 44 predicted open reading frames (ORFs) which comprised approximately 87% of the total sequence. Fifteen ORFs did not show any significant homology to known gene sequences. A number of ORFs show significant homology to plasmid-related genes, suggesting, at least in part, a plasmid origin for the SGI1, although some with homology to phage-related genes were identified. The SGI1 was identified in a number of multidrug-resistant DT120 and S. enterica serovar Agona strains with similar antibiotic-resistant phenotypes. The G+C content suggests a potential mosaic structure for the SGI1. Emergence of the SGI1 in serovar Agona strains is discussed.
authors.affiliation: National Microbiology Laboratory, Health Canada, Winnipeg, Manitoba R3E 3R2, Canada.
authors.forename: D
authors.initials: D
authors.lastname: Boyd
authors.forename: G A
authors.initials: GA
authors.lastname: Peters
authors.forename: A
authors.initials: A
authors.lastname: Cloeckaert
authors.forename: K S
authors.initials: KS
authors.lastname: Boumedine
authors.forename: E
authors.initials: E
authors.lastname: Chaslus-Dancla
authors.forename: H
authors.initials: H
authors.lastname: Imberechts
authors.forename: M R
authors.initials: MR
authors.lastname: Mulvey
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001434:Bacteriophage Typing; D001483:Base Sequence; D004352:Drug Resistance, Microbial / Q000235:genetics*; D018432:Drug Resistance, Multiple / Q000235:genetics; D016680:Genome, Bacterial; D006801:Humans; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D012483:Salmonella Phages / Q000235:genetics; D012486:Salmonella typhimurium / Q000145:classification / Q000187:drug effects / Q000235:genetics* / Q000821:virology; D017422:Sequence Analysis, DNA; D012703:Serotyping
chemical_list: D000900:Anti-Bacterial Agents