title: Cultivation of hamster kidney cells in a dynamic cell culture system in space (Spacelab IML-1 Mission).
abstract: Cell proliferation, tissue plasminogen activator (t-PA) production and metabolic changes of Hamster Kidney cells (HaK) grown on microcarriers in an automatic Dynamic Cell Culture System (DCCS) were determined on the first International Microgravity Mission (IML-1) Spacelab (22-30 January 1992). The DCCS was designed for two cell culture chambers (volume: 200 microliters each), one operating as a hatch system, the other as a perfusion system. Medium exchange was achieved with an osmotic pump (flow rate 1 microliter h-1). Two major items were investigated: the biological performance of the DCCS in space and the effect of microgravity on HaK cells. The results obtained demonstrated that (1) the DCCS can be used for biological experiments on long term Spacelab missions. In fact, higher cell densities and higher concentration of glucose but lower concentration of lactate in the perfusion chambers than in the batch chambers were measured. The concentration of t-PA, glutamine and ammonia was similar in all chambers. (2) Microgravity had no effect on cell growth and metabolism of HaK cells.
authors.affiliation: Space Biology Group, ETH-Technopark, Zurich, Switzerland.
authors.forename: G
authors.initials: G
authors.lastname: Lorenzi
authors.forename: F K
authors.initials: FK
authors.lastname: Gmunder
authors.forename: A
authors.initials: A
authors.lastname: Cogoli
mesh_terms: D000641:Ammonia / Q000378:metabolism; D000818:Animals; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D002455:Cell Division / Q000502:physiology; D002478:Cells, Cultured; D006224:Cricetinae; D003470:Culture Media; D003911:Dextrans; D005069:Evaluation Studies as Topic; D005947:Glucose / Q000378:metabolism; D005973:Glutamine / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007668:Kidney / Q000166:cytology*; D019344:Lactic Acid / Q000378:metabolism; D013026:Space Flight / Q000295:instrumentation*; D010959:Tissue Plasminogen Activator / Q000096:biosynthesis / Q000378:metabolism; D014893:Weightlessness
chemical_list: D003470:Culture Media; D003911:Dextrans; D005973:Glutamine; D019344:Lactic Acid; D000641:Ammonia; C052839:Cytodex; D010959:Tissue Plasminogen Activator; D005947:Glucose