[A plastic property in the change of rat muscle potentials clarified in the presence of neostigmine: a conjecture on the recycling process of ACh].
Released acetylcholine (ACh) is rapidly hydrolyzed, and choline is recaptured and re-used as available ACh for release. This elaborated recycling process of ACh was evaluated in terms of muscle potentials evoked by a set of 10 stimuli in the presence and the absence of neostigmine using Wistar rats anesthetized with urethane. All potentials evoked by a set of 10 stimuli before the injection of neostigmine were similar in amplitude. The first potential recorded 8 min after it was as large as that before it. The second potential was greatly depressed, and others were gradually recovered to the control level. The recovery was attained in the 5th-7th potential in some rats while a slight depression of the 10th potential was still seen in other animals. This time course was a general pattern in the change of muscle potentials evoked by a set of 10 stimuli in the presence of neostigmine. When 10 stimuli were given 9 min after it, all potentials showed rather comparable amplitudes to the control level. Thus, another pattern was found. The two patterns were repeatedly observed within individuals: A striking contrast in the changing pattern was noted between successive two sets of 10 stimuli with an interval of one minute. The former set exerted an influence upon those muscle potentials evoked by the latter one beyond one minute or so, but this effect disappeared during the elapse of two minutes or more. This plastic property and other findings were discussed in conjunction with those evidences recently accumulated on the recycling process of ACh. A conjecture was made on the recycling process of ACh: The process would be activated following impulse transmission, and this activation would be maintained for one minute or so. In that sense, this process would be controlled in a 'short-term memory' manner and endocytosis of synaptic vesicles coupled with their exocytosis should be emphasized. In addition, releasable ACh would be restored during repetitive stimulation even if it was once depleted notably.
Uramoto
I
I
Department of Physiology, Institute for Developmental Research, Aichi Human Service Center, Kasugai, Aichi 480-0392, Japan.
Watanabe
K
K
Totsuka
T
T
D000109:Acetylcholine / Q000378:metabolism*; D000818:Animals; D002800:Cholinesterase Inhibitors / Q000494:pharmacology*; D005071:Evoked Potentials / Q000187:drug effects*; D018482:Muscle, Skeletal / Q000502:physiology; D009388:Neostigmine / Q000494:pharmacology*; D009473:Neuronal Plasticity / Q000187:drug effects*; D051381:Rats
D002800:Cholinesterase Inhibitors; D009388:Neostigmine; D000109:Acetylcholine