Enhanced activation of B cells in a granulocyte colony-stimulating factor-mobilized peripheral blood stem cell graft.
In a randomized study that compared human leucocyte antigen-identical allogeneic granulocyte colony-stimulating factor (G-CSF)-mobilized peripheral blood stem cell (PBSC) versus bone marrow (BM) transplantation, the expression of activation markers, CD23, CD25 and CD45RO by B cells, was compared in blood before and after G-CSF mobilization and in PBSC versus BM grafts. The fractions of CD23+ and CD25+ B cells were higher in PBSC than in BM grafts. Moreover, we observed a G-CSF-induced increase in B-cell fractions in blood as well as in PBSC grafts when compared with BM grafts. Such an enhanced B-cell activation could contribute to the accelerated kinetics of immuno-haematological reconstitution, the occurrence of acute haemolysis in the ABO minor incompatibility setting, as well as the increased incidence of chronic graft-versus-host disease observed after PBSC transplantation.
Tayebi
H
H
Laboratoire de Thérapeutique Immuno-Moléculaire, INSERM, E-0119, UPRES EA-2284, Etablissement Français du Sang-Bourgogne/Franche Comté, Besançon, France.
Lapierre
V
V
Saas
P
P
Lienard
A
A
Sutton
L
L
Milpied
N
N
Attal
M
M
Cahn
J Y
JY
Kuentz
M
M
Blaise
D
D
Hervé
P
P
Tiberghien
P
P
Robinet
E
E
D001402:B-Lymphocytes / Q000276:immunology*; D015415:Biomarkers / Q000097:blood; D016026:Bone Marrow Transplantation; D005434:Flow Cytometry; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D019650:Hematopoietic Stem Cell Mobilization; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D017493:Leukocyte Common Antigens / Q000032:analysis; D008213:Lymphocyte Activation; D017455:Receptors, IgE / Q000032:analysis; D015375:Receptors, Interleukin-2 / Q000032:analysis; D014184:Transplantation, Homologous
D015415:Biomarkers; D017455:Receptors, IgE; D015375:Receptors, Interleukin-2; D016179:Granulocyte Colony-Stimulating Factor; D017493:Leukocyte Common Antigens