title: Fluorescence resonance energy transfer dye nucleotide terminators: a new synthetic approach for high-throughout DNA sequencing.
abstract: Fluorescence resonance energy transfer (FRET) based dye-nucleotide terminators (10-13) were designed, synthesized, and formulated with Thermo Sequenase II DNA polymerase into a robust kit for high throughput DNA sequencing. The key energy transfer (ET) rigid and linear linker (2), required for the syntheses of energy transfer cassettes (6-9) was synthesized via Heck coupling reaction on t-Boc-L-4-iodo-phenylalanine (1) with N-TFA-propargylamine.
authors.affiliation: Amersham Pharmacia Biotech, Piscataway, New Jersey 08855, USA.
authors.forename: S
authors.initials: S
authors.lastname: Nampalli
authors.forename: M
authors.initials: M
authors.lastname: Khot
authors.forename: J R
authors.initials: JR
authors.lastname: Nelson
authors.forename: P K
authors.initials: PK
authors.lastname: Flick
authors.forename: C W
authors.initials: CW
authors.lastname: Fuller
authors.forename: S
authors.initials: S
authors.lastname: Kumar
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000032:analysis; D004735:Energy Transfer; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000138:chemical synthesis*; D008969:Molecular Sequence Data; D009711:Nucleotides / Q000138:chemical synthesis*; D017422:Sequence Analysis, DNA / Q000379:methods*; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D005456:Fluorescent Dyes; D009711:Nucleotides; D004247:DNA