title: Binding of the Vestigial co-factor switches the DNA-target selectivity of the Scalloped selector protein.
abstract: The formation and identity of organs and appendages are regulated by specific selector genes that encode transcription factors that regulate potentially large sets of target genes. The DNA-binding domains of selector proteins often exhibit relatively low DNA-binding specificity in vitro. It is not understood how the target selectivity of most selector proteins is determined in vivo. The Scalloped selector protein controls wing development in Drosophila by regulating the expression of numerous target genes and forming a complex with the Vestigial protein. We show that binding of Vestigial to Scalloped switches the DNA-binding selectivity of Scalloped. Two conserved domains of the Vestigial protein that are not required for Scalloped binding in solution are required for the formation of the heterotetrameric Vestigial-Scalloped complex on DNA. We suggest that Vestigial affects the conformation of Scalloped to create a wing cell-specific DNA-binding selectivity. The modification of selector protein DNA-binding specificity by co-factors appears to be a general mechanism for regulating their target selectivity in vivo.
authors.affiliation: Howard Hughes Medical Institute and Laboratory of Molecular Biology, University of Wisconsin, 1525 Linden Drive, Madison,WI 53706, USA. ghalder@odin.mdacc.tmc.edu
authors.forename: G
authors.initials: G
authors.lastname: Halder
authors.forename: S B
authors.initials: SB
authors.lastname: Carroll
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D004247:DNA / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D019281:Dimerization; D029721:Drosophila Proteins; D004331:Drosophila melanogaster; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism*; D012996:Solutions; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D009687:Nuclear Proteins; D012996:Solutions; D014157:Transcription Factors; C087984:sd protein, Drosophila; C095064:vg protein, Drosophila; D004247:DNA