Immune induction and modulation in mice following immunization with DNA encoding F protein of respiratory syncytial virus.
Respiratory syncytial virus (RSV) is one of the principal agents of bronchiolitis and pneumonia in young children. Thus, there is a strong need to make a safe and effective vaccine against the RSV infection. DNA immunization is very effective at inducing both cellular and humoral immune responses. In this study, we inserted the RSV-F gene into expression vectors, pcDNA3.1 and pQE. These constructs were transformed into C2C12 and E. coli M15 cells, respectively. The expression of the RSV-F protein was confirmed by SDS-PAGE, followed by Western blot analyses. The immunization of pcDNA3.1-RSV-F elicited both anti-RSV-F titer in mouse sera and CTL activities with mouse splenocytes. Especially, the co-administration of IL-4, or the GM-CSF gene with the RSV-F gene construct, enhanced the production of anti-RSV-F Ab. However, this enhancement disappeared by the simultaneous injection of the Th1 and Th2 type cytokine genes. The CTL activities were affected by the co-delivery of the IFN-gamma gene, but not by Th2-type cytokines.
Park
E K
EK
Faculty of Biological Sciences, College of Natural Sciences, Chonbuk National University, Chonju, Korea.
Soh
B Y
BY
Jang
Y S
YS
Park
J H
JH
Chung
G H
GH
D000368:Aged; D000818:Animals; D000917:Antibody Formation / Q000276:immunology; D002460:Cell Line; D002648:Child; D003601:Cytotoxicity Tests, Immunologic; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics / Q000378:metabolism; D015847:Interleukin-4 / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008855:Microscopy, Electron, Scanning; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D022261:Respiratory Syncytial Virus Vaccines / Q000276:immunology*; D018113:Respiratory Syncytial Virus, Human / Q000235:genetics* / Q000276:immunology*; D014611:Vaccination; D019444:Vaccines, DNA / Q000276:immunology* / Q000494:pharmacology; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology*
D022261:Respiratory Syncytial Virus Vaccines; D019444:Vaccines, DNA; D014764:Viral Proteins; D015847:Interleukin-4; D007371:Interferon-gamma; D016178:Granulocyte-Macrophage Colony-Stimulating Factor