[Detection of microbes adherent to particles in a closed environment].
OBJECTIVE
In order to provide reference for controlling and monitoring the environmental contamination in the spacecraft, the species and amount of microbes, which adhere to the suspended particles with different size in a closed environment were detected.


METHODS
According to USA EPA IP-10, particles were continuously sampled by an impactor in a closed environment. The particles were divided into three grades by their aerodynamic diameters (AD): 100 micrometers > or = AD > 10 micrometers, 10 micrometers > or = AD>2 micrometers and AD < or =  2 micrometers. Clean outdoor air was collected as control by the same way. The collected particles were precisely weighed. The microbes that adhere to the particles were cultivated, counted and identified.


RESULTS
By calculating total suspended particles (TSP) and inhalant particles (IP) of the closed environment, counting, clarifying and identifying the microbes, 6 kinds of bacteria, 2 genus of actinomyces and 6 kinds of fungi that adhere to the particles were detected.


CONCLUSIONS
The particles in a closed environment may have some effect on human health and instruments.
Yu
F
F
Institute of Space Medico-Engineering, Beijing, China.
He
X X
XX
Xie
Q
Q
Jiang
J
J
D000190:Actinomyces / Q000145:classification / Q000302:isolation & purification*; D000337:Aerospace Medicine; D000393:Air Pollutants / Q000145:classification / Q000302:isolation & purification; D016902:Air Pollution, Indoor / Q000032:analysis*; D001419:Bacteria / Q000145:classification / Q000302:isolation & purification*; D018504:Ecological Systems, Closed; D004780:Environment, Controlled; D004783:Environmental Microbiology; D005658:Fungi / Q000145:classification / Q000302:isolation & purification*; D010316:Particle Size; D018531:Spacecraft
D000393:Air Pollutants