title: A new "native ligation" procedure for peptide-oligonucleotide conjugation.
abstract: "Native ligation", a powerful method of joining peptide fragments, has been applied successfully to peptide-oligonucleotide conjugation. Novel reagents are described for the solid-phase synthesis of peptide N-terminal thioesters and 5'-cysteinyl oligonucleotides suitable for ligation reactions.
authors.affiliation: Medical Research Council, Laboratory of Molecular Biology, Hills Road, CB2 2QH Cambridge, England.
authors.forename: D A
authors.initials: DA
authors.lastname: Stetsenko
authors.forename: M J
authors.initials: MJ
authors.lastname: Gait
mesh_terms: D003545:Cysteine / Q000031:analogs & derivatives*; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D009841:Oligonucleotides; D010455:Peptides; D003545:Cysteine