title: Automatic fixation facility for plant seedlings in the TEXUS Sounding Rocket Programme.
abstract: Automatic chemical fixation of plant seedlings within a 6 min period of reduced gravity (10(-4)g) was performed on three ballistic rocket flights provided by the German Sounding Rocket Programme TEXUS (Technologische Experimente unter Schwerelosigkeit = Technological Experiments in Microgravity). The described TEXUS experiment module consists of a standard experiment housing with batteries, cooling and heating systems, timer, and a data recording unit. Typically, 60 min before launch an experiment plug-in unit containing chambers with the plant material, the fixation system, and the temperature sensors is installed into the module which is already integrated in the payload section of the sounding rocket (late access). During the ballistic flight plant chambers are rapidly filled at pre-selected instants to preserve the cell structure of gravity sensing cells. After landing the plant material is processed for transmission electron microscopy. Up to now three experiments were successfully performed with cress roots (Lepidium sativum L.). Detailed improvements resulted in an automatic fixation facility which in principle can be used in unmanned missions.
authors.affiliation: Botanisches Institut der Universitat Bonn, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Tewinkel
authors.forename: J
authors.initials: J
authors.lastname: Burfeindt
authors.forename: P
authors.initials: P
authors.lastname: Rank
authors.forename: D
authors.initials: D
authors.lastname: Volkmann
mesh_terms: D000054:Acceleration; D019607:Brassicaceae; D004780:Environment, Controlled; D004867:Equipment Design; D005069:Evaluation Studies as Topic; D018466:Gravity Sensing; D018471:Hypergravity; D018517:Plant Roots / Q000166:cytology*; D013026:Space Flight / Q000295:instrumentation*; D018531:Spacecraft / Q000295:instrumentation; D016707:Tissue Fixation / Q000295:instrumentation / Q000379:methods*; D014893:Weightlessness