Inhibition of human telomerase by L-enantiomers of natural 2'-deoxyribonucleoside 5'-triphosphates.
In order to clarify whether L-enantiomers of natural 2'-deoxyribonucleoside 5'-triphosphates (dNTPs) are recognized by human telomerase, a quantitative telomerase assay based on the 'stretch PCR' method was developed and used for kinetic analysis. Among the four L-dNTPs, L-dTTP and L-dGTP inhibited telomerase activity and the others showed slight or no inhibitory effect. Lineweaver-Burk plot analysis showed that the inhibition mode L-dGTP was competitive with dGTP.
Yamaguchi
T
T
Department of Biological Sciences, Biotechnology Research Center, Teikyo University of Science and Technology, Uenohara, Yamanashi 409-0193, Japan.
Yamada
R
R
Tomikawa
A
A
Shudo
K
K
Saito
M
M
Ishikawa
F
F
Saneyoshi
M
M
D003848:Deoxyguanine Nucleotides / Q000378:metabolism / Q000494:pharmacology; D003854:Deoxyribonucleotides / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics; D016133:Polymerase Chain Reaction / Q000379:methods; D013237:Stereoisomerism; D019098:Telomerase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D013942:Thymine Nucleotides / Q000378:metabolism / Q000494:pharmacology
D003848:Deoxyguanine Nucleotides; D003854:Deoxyribonucleotides; D013942:Thymine Nucleotides; C029603:deoxyguanosine triphosphate; D019098:Telomerase; C024157:thymidine 5'-triphosphate