RGS8 protein is distributed in dendrites and cell body of cerebellar Purkinje cell.
RGS8 was originally identified as an RGS protein specifically expressed in neuronally differentiated P19 cells. We generated a polyclonal antibody specific to rat RGS8 using a synthetic peptide. When nonneural cells (DDT1MF2, CHO, and NIH3T3) transfected with rat RGS8 cDNA were immuno-stained with this antibody, the RGS8 protein was mainly detected in the nuclei. Since RGS8 mRNA was exclusively expressed in Purkinje cells of the cerebellum in the rat brain, we further examined the cellular distribution of the RGS8 protein in Purkinje cells using cultured cerebellar cells and tissue sections of the cerebellum. The RGS8 protein was excluded from the nuclei and distributed in the cell body and dendrites, but not in the axons of Purkinje cells. These results demonstrate the presence of a mechanism controlling the distribution of RGS8 protein in cerebellar Purkinje cells.
Itoh
M
M
Department of Molecular Cell Signaling, Tokyo Metropolitan Institute for Neuroscience, 2-6 Musashidai, Fuchu-shi, Tokyo 183-8526, Japan.
Odagiri
M
M
Abe
H
H
Saitoh
O
O
D016475:3T3 Cells; D000818:Animals; D000906:Antibodies; D016466:CHO Cells; D002525:Cerebellar Cortex / Q000166:cytology; D006224:Cricetinae; D003713:Dendritic Cells / Q000378:metabolism*; D008565:Membrane Proteins / Q000276:immunology / Q000378:metabolism; D051379:Mice; D011689:Purkinje Cells / Q000378:metabolism*; D020710:RGS Proteins / Q000276:immunology / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013347:Subcellular Fractions
D000906:Antibodies; D008565:Membrane Proteins; D020710:RGS Proteins; C486485:Rgs8 protein, rat