title: Endomucin is expressed in embryonic dorsal aorta and is able to inhibit cell adhesion.
abstract: Recent studies have suggested the existence of progenitors common to hematopoietic and endothelial cells, called hemangioblasts, in, for instance, embryonic dorsal aorta. To identify a membrane-bound or secretory molecule regulating early hematopoiesis, we screened a cDNA library from dorsal aortas of embryonic day (E) 10.5 mice by a signal sequence trap method and obtained a clone encoding a sialoprotein, endomucin-1. Immunohistochemistry revealed that the endomucin-1 transcript was specifically expressed in the endothelial cells of dorsal aorta of E10.5 mouse embryo. Overexpression of endomucin-1 strongly inhibited adhesion and aggregation of cells, including cultured endothelial cells from E10.5 dorsal aorta. These data suggest that endomucin-1 may play a role in detachment of hematopoietic cells from endothelium during early hematopoiesis.
authors.affiliation: Department of Cell Fate Modulation, Institute of Molecular Embryology and Genetics, Kumamoto University, 2-2-1, Honjo, Kumamoto 860-0811, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Ueno
authors.forename: K
authors.initials: K
authors.lastname: Igarashi
authors.forename: N
authors.initials: N
authors.lastname: Kimura
authors.forename: K
authors.initials: K
authors.lastname: Okita
authors.forename: M
authors.initials: M
authors.lastname: Takizawa
authors.forename: I
authors.initials: I
authors.lastname: Nobuhisa
authors.forename: T
authors.initials: T
authors.lastname: Kojima
authors.forename: T
authors.initials: T
authors.lastname: Kitamura
authors.forename: U
authors.initials: U
authors.lastname: Samulowitz
authors.forename: D
authors.initials: D
authors.lastname: Vestweber
authors.forename: T
authors.initials: T
authors.lastname: Shimomura
authors.forename: T
authors.initials: T
authors.lastname: Suda
authors.forename: K
authors.initials: K
authors.lastname: Nakashima
authors.forename: T
authors.initials: T
authors.lastname: Taga
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001011:Aorta / Q000166:cytology / Q000378:metabolism; D001483:Base Sequence; D002448:Cell Adhesion / Q000502:physiology*; D002449:Cell Aggregation / Q000502:physiology; D018076:DNA, Complementary / Q000032:analysis; D004622:Embryo, Mammalian / Q000378:metabolism; D004730:Endothelium, Vascular / Q000378:metabolism / Q000502:physiology*; D006412:Hematopoietic Stem Cells / Q000502:physiology; D051379:Mice; D008969:Molecular Sequence Data; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000032:analysis; D012333:RNA, Messenger / Q000235:genetics; D016415:Sequence Alignment; D012795:Sialoglycoproteins / Q000096:biosynthesis* / Q000502:physiology
chemical_list: D018076:DNA, Complementary; C498985:Emcn protein, mouse; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D012333:RNA, Messenger; D012795:Sialoglycoproteins