[Marker transport across biological barriers in vitro: comparison of cell culture models for the gastrointestinal barrier, the blood-brain barrier and the alveolar epithelium of the lung].
In order to respond to the flood of new active ingredients currently being generated by combinatorial chemistry or molecular biological synthesis, selection procedures able to filter out rapidly and economically those drug candidates with the highest development potential are required. This necessitates the measurement of fundamental biopharmaceutical parameters very early in the drug development process. Any pharmaceutically active agent must be able to overcome the body's natural protective mechanisms. A broad variety of biological barriers can be simulated in the laboratory by cell monolayer models. Apart from ethical aspects, the advantage of these in vitro test systems is that permeability studies can be performed at high throughput rates under controlled and reproducible conditions. The validity of such a model is ultimately reflected in its ability to accurately predict the behaviour of an active ingredient at the corresponding in vivo barrier.
Gindorf
C
C
Across Barriers GmbH, Science Park Saar, Postfach D-Saarbrücken, Science Park Saar, Postfach, Germany.
Steimer
A
A
Lehr
C M
CM
Bock
U
U
Schmitz
S
S
Haltner
E
E
D000826:Animal Testing Alternatives; D000818:Animals; D001812:Blood-Brain Barrier; D018929:Cell Culture Techniques / Q000379:methods; D002460:Cell Line; D002463:Cell Membrane Permeability; D004068:Digestive System Physiological Phenomena; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D006801:Humans; D008954:Models, Biological; D004364:Pharmaceutical Preparations / Q000378:metabolism*; D011650:Pulmonary Alveoli / Q000502:physiology*; D020545:Respiratory Mucosa / Q000502:physiology*; D014407:Tumor Cells, Cultured
D004364:Pharmaceutical Preparations