SEM visualization of glycosylated surface molecules using lectin-coated microspheres.
There are several techniques currently used to localize glycosylated surface molecules by scanning electron microscopy (Grinnell, 1980; Molday, 1976; Linthicum and Sell, 1975; Nicolson, 1974; Lo Buglio, et al, 1972).  A simple and rapid method, using a modification of Grinnell's technique is reported here.  Essentially, microspheres coated with Concavalin A are used to bind to glycosylated regions of the palatal shelf epithelium and are visualized in the scanning electron microscope (SEM).
Duke
J
J
University of Texas Dental Branch, Houston 77225, USA.
Janer
L
L
Campbell
M
M
D000818:Animals; D003208:Concanavalin A / Q000032:analysis / Q000737:chemistry*; D004847:Epithelial Cells; D004848:Epithelium / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure; D006031:Glycosylation; D037102:Lectins / Q000032:analysis / Q000737:chemistry*; D008565:Membrane Proteins / Q000032:analysis* / Q000378:metabolism / Q000648:ultrastructure*; D051379:Mice; D008813:Mice, Inbred ICR; D008855:Microscopy, Electron, Scanning / Q000379:methods*; D008863:Microspheres; D010159:Palate / Q000737:chemistry / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure
D037102:Lectins; D008565:Membrane Proteins; D003208:Concanavalin A