title: Comparative performance of Colisure (TM) and accepted methods in the detection of chlorine-injured total coliforms and E.coli.
abstract: Studies were done to examine the comparability of Colisure (TM) and accepted reference methods to detect low numbers of total coliform bacteria and E.coli subjected to chlorine stress.  Colisure (TM) is a medium designed to concurrently detect coliform bacteria and E.coli in drinking water by the specific action of beta-galactosidase (total coliforms) and beta-glucuronidase (E.coli).  The methods used to compare the performance of various media followed a protocol established by the USEPA.  Samples (31) of sewage from six different regions of the US were treated with sufficient concentrations of chlorine (1.2-2.5mg/l) to reduce viability 1-3 logs (39% average injury) and diluted with drinking water to achieve ca. 3 viable coliforms/100ml.  The mean log reductions in viable bacteria, determined with various media following disinfection of the 31 samples were:  mEndo = 1.87 (TC), Colisure (TM) = 1.55 (TC), mTec = 3.63 (E.coli) and Colisure (TM) = 2.01 (E.coli).  When Colisure (TM) was compared with accepted methods to detect total coliforms in the dilute, disinfected samples, Colisure (TM) yielded results that were 1.6 times greater than LTB confirmed in BGLB at 28h.  Colisure (TM) also detected 1.7 times greater levels of E.coli than LTB confirmed in EC/MUG at 28h.  Sensitivity and specificity of Colisure (TM) were between 96 and 100% when positive and negative tests were verified.  These findings indicate that Colisure (TM) is superior to certain accepted reference methods in the detection of chlorine-injured coliforms and E.coli under conditions that resemble contaminated drinking water.
authors.affiliation: Microbiology Department, Montana State University, Bozeman 59717, USA.
authors.forename: G A
authors.initials: GA
authors.lastname: McFeters
authors.forename: S C
authors.initials: SC
authors.lastname: Broadaway
authors.forename: B H
authors.initials: BH
authors.lastname: Pyle
authors.forename: M
authors.initials: M
authors.lastname: Pickett
authors.forename: Y
authors.initials: Y
authors.lastname: Egozy
mesh_terms: D001431:Bacteriological Techniques; D002713:Chlorine / Q000009:adverse effects*; D004796:Clinical Enzyme Tests; D015169:Colony Count, Microbial; D004202:Disinfectants / Q000009:adverse effects; D004755:Enterobacteriaceae / Q000187:drug effects / Q000201:enzymology / Q000302:isolation & purification*; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology / Q000302:isolation & purification*; D005069:Evaluation Studies as Topic; D012722:Sewage / Q000032:analysis / Q000382:microbiology; D014871:Water Microbiology / Q000592:standards*; D014881:Water Supply / Q000032:analysis* / Q000592:standards; D001616:beta-Galactosidase / Q000032:analysis
chemical_list: D004202:Disinfectants; D012722:Sewage; D002713:Chlorine; D001616:beta-Galactosidase