title: [Fluorescent analysis of irradiation-induced changes in chromatin state].
abstract: A fluorescent technique with Hoechst-33258 and acridine orange staining was used to assess changes in chromatin state induced by radiation. Fluorochromes with different modes of binding to DNA were chosen. In T lymphocytes chronic irradiation caused a rearrangement of binding between nonhiston proteins and lipids accompanied by conformational changes in DNA, resulting in chromatin condensation. The decrease in fluorescence probably resulted from a reduction in the number of sites accessible for dye binding. After acute irradiation, the fluorescence intensity decreases predominantly due to double-strand breaks.
authors.affiliation: Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, 142290 Russia.
authors.forename: R Ia
authors.initials: RIa
authors.lastname: Gordon
authors.forename: E G
authors.initials: EG
authors.lastname: Novoselova
authors.forename: N L
authors.initials: NL
authors.lastname: Kruglova
authors.forename: E V
authors.initials: EV
authors.lastname: Mel'nikova
authors.forename: S S
authors.initials: SS
authors.lastname: Khutsian
mesh_terms: D000818:Animals; D006690:Bisbenzimidazole; D002843:Chromatin / Q000378:metabolism / Q000528:radiation effects*; D004247:DNA / Q000737:chemistry / Q000528:radiation effects; D004249:DNA Damage; D005456:Fluorescent Dyes; D005720:Gamma Rays; D008297:Male; D015530:Nuclear Matrix / Q000528:radiation effects; D051381:Rats; D017208:Rats, Wistar; D013154:Spleen / Q000737:chemistry / Q000166:cytology / Q000528:radiation effects; D013601:T-Lymphocytes / Q000378:metabolism / Q000528:radiation effects
chemical_list: D002843:Chromatin; D005456:Fluorescent Dyes; D004247:DNA; D006690:Bisbenzimidazole