Novel cis-acting replication element in the adeno-associated virus type 2 genome is involved in amplification of integrated rep-cap sequences.
This study identifies a region of the adeno-associated virus type 2 (AAV-2) rep gene (nucleotides 190 to 540 of wild-type AAV-2) as a cis-acting Rep-dependent element able to promote the replication of transiently transfected plasmids. This viral element is also shown to be involved in the amplification of integrated sequences in the presence of adenovirus and Rep proteins.
Nony
P
P
Laboratoire de Thérapie Génique, CHU Hotel Dieu, 44035 Nantes Cedex 01, France.
Tessier
J
J
Chadeuf
G
G
Ward
P
P
Giraud
A
A
Dugast
M
M
Linden
R M
RM
Moullier
P
P
Salvetti
A
A
D001483:Base Sequence; D004279:DNA, Viral / Q000235:genetics; D000229:Dependovirus / Q000235:genetics*; D016679:Genome, Viral; D008969:Molecular Sequence Data; D010957:Plasmids; D014162:Transfection; D014764:Viral Proteins / Q000235:genetics; D016662:Virus Integration / Q000235:genetics; D014779:Virus Replication
D004279:DNA, Viral; D014764:Viral Proteins