A test battery for the ecotoxicological evaluation of pentachlorophenol.
Experimental bioassays are currently used in ecotoxicology and environmental toxicology to provide information for risk assessment evaluation of new chemicals and to investigate their effects and mechanisms of action; in addition, ecotoxicological models are used for the detection, control and monitoring of the presence of pollutants in the environment. As a single bioassay will never provide a full picture of the quality of the environment, a representative, cost-effective and quantitative test battery should be developed. The effects of pentachlorophenol were studied using a battery of ecotoxicological model systems, including immobilization of Daphnia magna, bioluminiscence inhibition in the bacterium Vibrio fischeri, growth inhibition of the alga Chlorella vulgaris, and micronuclei induction in the plant Allium cepa. The inhibition of cell proliferation and MTT reduction were investigated in Vero cells. Neutral red uptake, cell growth, MTT reduction, lactate dehydrogenase leakage and activity were studied in the salmonid fish cell line RTG-2, derived from the gonad of rainbow trout. Pentachlorophenol was very toxic for all biota and cells. The system most sensitive to pentachlorophenol, was micronuclei induction in A. cepa, followed by D. magna immobilization, bioluminescence inhibition in V. fischeri bacteria at 60 min and cell proliferation inhibition of RTG-2 cells at 72 h. Inhibition of cell proliferation and MTT reduction on Vero monkey cells showed intermediate sensitivity.
Repetto
G
G
National Institute of Toxicology, PO Box 863, 41080 Seville, Spain. repetto@sev.inaltox.es
Jos
A
A
Hazen
M J
MJ
Molero
M L
ML
del Peso
A
A
Salguero
M
M
Castillo
P D
PD
Rodríguez-Vicente
M C
MC
Repetto
M
M
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002708:Chlorella / Q000187:drug effects / Q000254:growth & development; D002522:Chlorocebus aethiops; D003621:Daphnia / Q000187:drug effects / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D017753:Ecosystem; D004785:Environmental Pollutants / Q000633:toxicity*; D066298:In Vitro Techniques; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008163:Luminescent Measurements; D048629:Micronuclei, Chromosome-Defective / Q000187:drug effects; D008954:Models, Biological; D009499:Neutral Red / Q000378:metabolism; D017686:Oncorhynchus mykiss; D019697:Onions / Q000187:drug effects / Q000235:genetics; D010416:Pentachlorophenol / Q000633:toxicity*; D013778:Tetrazolium Salts / Q000378:metabolism; D018675:Toxicity Tests / Q000379:methods*; D014709:Vero Cells; D014733:Vibrio / Q000187:drug effects / Q000502:physiology
D004785:Environmental Pollutants; D013778:Tetrazolium Salts; D009499:Neutral Red; D010416:Pentachlorophenol; D007770:L-Lactate Dehydrogenase