title: Adjuvant effects of IL-1beta, IL-2, IL-8, IL-15, IFN-alpha, IFN-gamma TGF-beta4 and lymphotactin on DNA vaccination against Eimeria acervulina.
abstract: Eight chicken cytokine genes (IL-1beta, IL-2, IL-8, IL-15, IFN-alpha, IFN-gamma, TGF-beta4, lymphotactin) were evaluated for their adjuvant effect on a suboptimal dose of an Eimeria DNA vaccine carrying the 3-1E parasite gene (pcDNA3-1E). Chickens were given two subcutaneous injections with 50 microg of the pcDNA3-1E vaccine plus a cytokine expression plasmid 2 weeks apart and challenged with Eimeria acervulina 1 week later. IFN-alpha (1 microg) or 10 microg of lymphotactin expressing plasmids, when given simultaneously with the pcDNA3-1E vaccine, significantly protected against body weight loss induced by E. acervulina. Parasite replication was significantly reduced in chickens given the pcDNA3-1E vaccine along with 10 microg of the IL-8, lymphotactin, IFN-gamma, IL-15, TGF-beta4, or IL-1beta plasmids compared with chickens given the pcDNA3-1E vaccine alone. Flow cytometric analysis of duodenum intraepithelial lymphocytes showed chickens that received the pcDNA3-1E vaccine simultaneously with the IL-8 or IL-15 genes had significantly increased CD3+ cells compared with vaccination using pcDNA3-1E alone or in combination with the other cytokine genes tested. These results indicate that the type and the dose of cytokine genes injected into chickens influence the quality of the local immune response to DNA vaccination against coccidiosis.
authors.affiliation: Parasite Biology, Epidemiology, Systematics Laboratory, Animal and Natural Resources Institute, BARC-East, Building 1040, US Department of Agriculture, Beltsville, MD 20705, USA.
authors.forename: W
authors.initials: W
authors.lastname: Min
authors.forename: H S
authors.initials: HS
authors.lastname: Lillehoj
authors.forename: J
authors.initials: J
authors.lastname: Burnside
authors.forename: K C
authors.initials: KC
authors.lastname: Weining
authors.forename: P
authors.initials: P
authors.lastname: Staeheli
authors.forename: J J
authors.initials: JJ
authors.lastname: Zhu
mesh_terms: D000276:Adjuvants, Immunologic; D000818:Animals; D002645:Chickens; D003048:Coccidiosis / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D004353:Drug Evaluation, Preclinical; D004386:Duodenum / Q000276:immunology / Q000469:parasitology; D004539:Eimeria / Q000276:immunology*; D005822:Genetic Vectors / Q000008:administration & dosage / Q000235:genetics; D016898:Interferon-alpha / Q000235:genetics / Q000276:immunology; D007371:Interferon-gamma / Q000235:genetics / Q000276:immunology; D007372:Interferons / Q000235:genetics / Q000276:immunology*; D007375:Interleukin-1 / Q000235:genetics / Q000276:immunology; D019409:Interleukin-15 / Q000235:genetics / Q000276:immunology; D007376:Interleukin-2 / Q000235:genetics / Q000276:immunology; D016209:Interleukin-8 / Q000235:genetics / Q000276:immunology; D007378:Interleukins / Q000235:genetics / Q000276:immunology*; D008222:Lymphokines / Q000235:genetics / Q000276:immunology*; D010270:Parasite Egg Count; D011201:Poultry Diseases / Q000276:immunology / Q000517:prevention & control*; D012795:Sialoglycoproteins / Q000235:genetics / Q000276:immunology*; D013047:Specific Pathogen-Free Organisms; D016212:Transforming Growth Factor beta / Q000235:genetics / Q000276:immunology*; D014611:Vaccination / Q000662:veterinary; D019444:Vaccines, DNA / Q000235:genetics / Q000276:immunology; D015430:Weight Gain
chemical_list: D000276:Adjuvants, Immunologic; D016898:Interferon-alpha; D007375:Interleukin-1; D019409:Interleukin-15; D007376:Interleukin-2; D016209:Interleukin-8; D007378:Interleukins; D008222:Lymphokines; D012795:Sialoglycoproteins; D016212:Transforming Growth Factor beta; D019444:Vaccines, DNA; C018997:lymphotactin; C445082:transforming growth factor beta4; D007371:Interferon-gamma; D007372:Interferons