title: Primary structure of alpha-globin chains from river buffalo (Bubalus bubalis L.) hemoglobins.
abstract: Primary structure analysis of the four river buffalo alpha-globin chains showed that haplotypes A and B differ from each other by a substitution at codon 64 that may encode Ala or Asn. The A haplotype encodes two alpha-globin chains, Ialpha1 and IIalpha3, which differ at positions 129 and 131: Ialpha1 has 64 Ala, 129 Phe, 131 Asn; IIalpha3 has 64 Ala, 129 Leu, 131 Ser. The B haplotype encodes two alpha-globin chains, Ialpha2 and IIalpha4, which differ at positions 10 and 11: Ialpha2 has 10 I1e, 11 Gln, 64 Asn; IIalpha4 has 10 Val, 11 Lys, 64 Asn. Apart from the Ala/Asn polymorphism at position 64, amino acid substitutions in allelic and nonallelic alpha-globin chains seem to have arisen by single point mutations. Detection of electrophoretically silent mutations due to neutral amino acid substitutions and their influence on the isoelectric point are discussed. Furthermore, primary structures of river buffalo alpha-globin chains are compared to other species of the Bovidae family to suggest evolutionary events that have characterized the amino acid substitutions of river buffalo hemoglobin.
authors.affiliation: Dipartimento di Scienza degli Alimenti, Università Federico II, Naples, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Ferranti
authors.forename: A
authors.initials: A
authors.lastname: Facchiano
authors.forename: F
authors.initials: F
authors.lastname: Zappacosta
authors.forename: D
authors.initials: D
authors.lastname: Vincenti
authors.forename: R
authors.initials: R
authors.lastname: Rullo
authors.forename: B
authors.initials: B
authors.lastname: Masala
authors.forename: A
authors.initials: A
authors.lastname: Di Luccia
mesh_terms: D000595:Amino Acid Sequence; D019943:Amino Acid Substitution / Q000235:genetics; D000818:Animals; D002020:Buffaloes / Q000097:blood*; D002268:Carboxypeptidases / Q000494:pharmacology; D043422:Carboxypeptidases A; D002417:Cattle; D019143:Evolution, Molecular; D005914:Globins / Q000032:analysis / Q000737:chemistry* / Q000235:genetics*; D006239:Haplotypes / Q000235:genetics; D007525:Isoelectric Focusing / Q000379:methods; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010641:Phenotype; D011110:Polymorphism, Genetic / Q000187:drug effects
chemical_list: D005914:Globins; D002268:Carboxypeptidases; D043422:Carboxypeptidases A