Reinitiation of vegetative growth from aseptically cultured terminal floral apex of banana.
Terminal floral apices of Musa acuminata cv. Dwarf Cavendish were isolated and cultured in a modified Murashige and Skoog (1962) medium supplemented with N6-benzylamino purine (5 mg/L) and 10% (v/v) coconut water.  Under these conditions, the determinate floral buds are transformed into a multiplying vegetative shoot system.  Rooted plantlets were obtained by treating shoots with the auxin, naphthaleneacetic acid (1 mg/L), and activated charcoal (0.025%).  Practical benefits resulting from these observations, such as the production of virus-free plants and the rapid multiplication of stock material, are discussed.
Cronauer
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Department of Biochemistry, State University of New York at Stony Brook 11794, USA.
Krikorian
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D001207:Asepsis; D002606:Charcoal / Q000494:pharmacology; D003470:Culture Media; D046508:Culture Techniques / Q000379:methods; D005638:Fruit / Q000166:cytology / Q000187:drug effects / Q000254:growth & development*; D007210:Indoleacetic Acids / Q000494:pharmacology; D009280:Naphthaleneacetic Acids / Q000494:pharmacology; D018520:Plant Shoots / Q000166:cytology / Q000187:drug effects / Q000254:growth & development*
D003470:Culture Media; D007210:Indoleacetic Acids; D009280:Naphthaleneacetic Acids; D002606:Charcoal; C034182:1-naphthaleneacetic acid