Differential transcription factor expression in human mononuclear cells in response to amphotericin B: identification with complementary DNA microarray technology.
OBJECTIVE
To identify genes differentially expressed in human monocytic cells exposed to amphotericin B in vitro.


METHODS
In vitro experiment.


METHODS
Hospital laboratory.


METHODS
Human mononuclear cell line, THP-1.


METHODS
Human mononuclear cells were exposed to amphotericin B or media alone for 6 hours. After exposure, total RNA was isolated and reverse transcribed to complementary DNA. Differences in probe hybridization observed during blotting were measured, and genes with altered regulation were described by using human complementary DNA microarray technology.


RESULTS
Of 588 genes represented on the array, 16 transcripts were found to be upregulated and 4 transcripts were downregulated in response to amphotericin B. These findings suggest that amphotericin B alters the expression of genes in human monocytic cells that play a role in many cellular functions, including immune response, signal transduction, and cell differentiation.


CONCLUSIONS
Amphotericin B induces alterations in human cell gene transcription. These changes could be used to evaluate differences in toxicity or efficacy observed in patients receiving this agent.
Cleary
J D
JD
Department of Clinical Pharmacy Practice, University of Mississippi School of Pharmacy, Jackson 39216, USA. Jcleary@medicine.umsmed.edu
Rogers
P D
PD
Chapman
S W
SW
D000666:Amphotericin B / Q000494:pharmacology*; D000935:Antifungal Agents / Q000494:pharmacology*; D000954:Antigens, Surface / Q000378:metabolism; D001345:Autoradiography; D002478:Cells, Cultured; D018076:DNA, Complementary / Q000096:biosynthesis*; D006801:Humans; D007202:Indicators and Reagents; D008567:Membranes, Artificial; D009000:Monocytes / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D011956:Receptors, Cell Surface / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology; D013315:Stress, Psychological; D014157:Transcription Factors / Q000096:biosynthesis*
D000935:Antifungal Agents; D000954:Antigens, Surface; D018076:DNA, Complementary; D007202:Indicators and Reagents; D008567:Membranes, Artificial; D011956:Receptors, Cell Surface; D014157:Transcription Factors; D000666:Amphotericin B