title: The structure and function of a gene encoding a basic peptide from prawn white spot syndrome virus.
abstract: Prawn white spot syndrome virus (WSSV) is the major pathogen responsible for the high mortality of cultured prawns. A gene (termed as p6.8) encoding a basic peptide was found by screening the cDNA and DNA libraries of WSSV. The peptide was highly homologous with proteins rich in arginine and lysine. A fusion protein containing the p6.8 and green fluorescent protein (GFP) genes was cloned into pBV220 and expressed in E. coli. Gel mobility shift assay indicated that the peptide encoded by p6.8 had the capability of binding DNA and might be involved in DNA packaging.
authors.affiliation: Key Laboratory of Marine Biotechnology, The Third Institute of Oceanography, State Oceanic Administration, 361005, Xiamen, People's Republic of China.
authors.forename: X
authors.initials: X
authors.lastname: Zhang
authors.forename: X
authors.initials: X
authors.lastname: Xu
authors.forename: C L
authors.initials: CL
authors.lastname: Hew
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004267:DNA Viruses / Q000235:genetics*; D004279:DNA, Viral; D004268:DNA-Binding Proteins / Q000235:genetics*; D033364:Decapoda / Q000821:virology*; D015870:Gene Expression; D005814:Genes, Viral; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010455:Peptides / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics*
chemical_list: C438523:C36 protein, prawn white spot syndrome virus; D004279:DNA, Viral; D004268:DNA-Binding Proteins; D010455:Peptides; D014764:Viral Proteins