title: Mechanism of phosphorylation of the regulatory light chain of myosin from tarantula striated muscle.
abstract: Contraction is modulated in many striated muscles by Ca2+-calmodulin dependent phosphorylation of the myosin regulatory light chain (RLC) by myosin light chain kinase. We have investigated the biochemical mechanism of RLC phosphorylation in tarantula muscle to better understand the basis of myosin-linked regulation. In an earlier study it was concluded that the RLC occurred as two species, both of which could be phosphorylated, potentiating contraction. Here we present evidence that only a single species exists, and that this can be phosphorylated at one or two sites. In relaxed muscle we find evidence for a substantial level of basal phosphorylation at the first site. This is augmented on activation, followed by partial phosphorylation of the second site. We find in addition that Ca2+ has a dual effect on light chain phosphorylation, depending on its concentration. At low concentration (relaxing conditions) only basal phosphorylation is observed, while at higher concentrations (activating conditions) RLC phosphorylation is stimulated. At still higher Ca2+ concentrations we find partial inhibition of RLC phosphorylation, suggesting an additional mechanism by which the muscle cell can fine tune contractile activity by controlling the level of free Ca2+.
authors.affiliation: Departamento de Biología Estructural, Instituto Venezolano de Investigaciones Científicas, Caracas. Carlos.Hidalgo@umassmed.edu
authors.forename: C
authors.initials: C
authors.lastname: Hidalgo
authors.forename: R
authors.initials: R
authors.lastname: Craig
authors.forename: M
authors.initials: M
authors.lastname: Ikebe
authors.forename: R
authors.initials: R
authors.lastname: Padrón
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D009119:Muscle Contraction / Q000187:drug effects; D018994:Myosin Light Chains / Q000378:metabolism*; D009219:Myosin-Light-Chain Kinase / Q000378:metabolism*; D009994:Osmolar Concentration; D010710:Phosphates / Q000494:pharmacology; D010766:Phosphorylation / Q000187:drug effects; D011499:Protein Processing, Post-Translational / Q000187:drug effects; D013112:Spiders / Q000378:metabolism*
chemical_list: D018994:Myosin Light Chains; D010710:Phosphates; D000255:Adenosine Triphosphate; D009219:Myosin-Light-Chain Kinase; D002118:Calcium