Lipoplex-mediated stable gene transfer into HeLa cells.
Unilamellar cationic liposomes containing phosphatidylcholine, L-alpha-phosphatidyl-DL-glycerol, cholesterol and N,N-dimethylaminopropylaminyl succinyl cholesterol in lipoplexes with plasmid ptkNEO transfected HeLa cells efficiently in the presence of G418.
Singh
M
M
Department of Biochemistry, University of Durban-Westville, Private Bag X54001, Durban 4000, South Africa.
Balram
S
S
Ariatti
M
M
D002784:Cholesterol / Q000031:analogs & derivatives; D006367:HeLa Cells; D006801:Humans; D008081:Liposomes; D010713:Phosphatidylcholines; D010715:Phosphatidylglycerols; D010957:Plasmids; D014162:Transfection / Q000379:methods*
D008081:Liposomes; D010713:Phosphatidylcholines; D010715:Phosphatidylglycerols; D002784:Cholesterol