title: Comparison of the prevalence of glutamic acid decarboxylase (GAD65) and gliadin antibodies (AGA) in a randomly selected adult estonian population.
abstract: We aimed to test the hypothesis that gluten might be associated with the development of islet cell autoimmunity. A random sample of 200 persons (87 males, mean age 42.4 years) from Estonia including one patient with type I diabetes mellitus was studied. IgG-type glutamic acid decarboxylase (GAD65) antibodies were determined using radioligand-binding assay and IgG/IgA-type gliadin antibodies (AGA) by enzyme-linked immunosorbent assay. Generic HLA-DRB1* alleles were analyzed using a polymerase chain reaction. Although our results revealed the highest GAD65Ab index and a high IgA-type AGA in a person with diabetes, no correlation between GAD65Ab and AGA values was revealed among the other 199 persons (p > 0.05). There were also no differences between test values among persons with and without different HLA-DRB1* alleles (p > 0.05). In the GAD65Ab assay, one person (0.5 %; 95 % CI: 0 - 1.5) out of 199 exceeded the 99(th) centile of the GAD65Ab index. In summary, the present study does not confirm the possibility that there is a relationship between the immune reactivity against GAD65 and gliadin, at least in persons without type I DM.
authors.affiliation: Department of Immunology, University of Tartu, 19 Ravila Street, Tartu 51014, Estonia.
authors.forename: R
authors.initials: R
authors.lastname: Uibo
authors.forename: E P
authors.initials: EP
authors.lastname: Sullivan
authors.forename: O
authors.initials: O
authors.lastname: Uibo
authors.forename: A
authors.initials: A
authors.lastname: Lernmark
authors.forename: L
authors.initials: L
authors.lastname: Salur
authors.forename: T
authors.initials: T
authors.lastname: Kivik
authors.forename: M
authors.initials: M
authors.lastname: Mandel
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000483:Alleles; D001323:Autoantibodies / Q000097:blood*; D003922:Diabetes Mellitus, Type 1 / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D004957:Estonia; D005260:Female; D005903:Gliadin / Q000276:immunology*; D005968:Glutamate Decarboxylase / Q000276:immunology*; D006684:HLA-DR Antigens / Q000235:genetics; D059811:HLA-DRB1 Chains; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D007515:Islets of Langerhans / Q000276:immunology; D008297:Male; D008875:Middle Aged
chemical_list: D001323:Autoantibodies; D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D005903:Gliadin; D005968:Glutamate Decarboxylase