title: Induction of asymmetrical type of chromosomal aberrations in cultured human lymphocytes by ion beams of different energies at varying LET from HIMAC and RRC.
abstract: Frequencies of asymmetrical type of chromosome aberration were scored in cultured human blood lymphocytes irradiated with carbon and neon beams. Blood cells were irradiated with various doses to establish dose response curves for chromosome aberration frequency vs. dose, and chromosome preparation was made by conventional method. Dose response curves for the per cell frequencies of the dicentrics and centric rings as well as the excess amount of acentric fragments were described for 7 different qualities (LET = 22.4, 40.0, 41.5, 69.9, 70.0, 100.0 and 150 KeV/micrometer) of carbon and neon beams with three different energies, 135, 290 and 400 MeV/u. From the analysis of those dose response curves, the maximum effect was found in the region of LET value at near 70 KeV/micrometer together with linear expression in the response from all endpoints examined. The 135 MeV/u of carbons (69.9 KeV/micrometer) and neons(70.0 KeV/micrometer) showed linear response. The 290 MeV/u of carbons (100 KeV/m) and neons (150 KeV/micrometer) showed medium effects with different shape of response, linear with a plateau and upward concavity. The 2 carbon beams (41.5 and 40 KeV/micrometer) from 2 different accelerators showed much discrepancy in the response. RBE-LET relationship was also described by comparing the coefficient alpha of the 7 different dose responses. The peak (near 70 KeV/m) was localized close to that (80 KeV/m) for the survivals of dsb repair deficient cells (Eguchi-Kasai et al. 1998), but in different position from that previously reported in many other studies (100-200 KeV/mm). Identification of the RBEmax in the present study has yet to be definitive.
authors.affiliation: Department of Biology, Okayama University, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Ohara
authors.forename: N
authors.initials: N
authors.lastname: Okazaki
authors.forename: M
authors.initials: M
authors.lastname: Monobe
authors.forename: S
authors.initials: S
authors.lastname: Watanabe
authors.forename: M
authors.initials: M
authors.lastname: Kanayama
authors.forename: M
authors.initials: M
authors.lastname: Minamihisamatsu
mesh_terms: D002244:Carbon; D002478:Cells, Cultured; D002869:Chromosome Aberrations / Q000502:physiology*; D004249:DNA Damage; D004307:Dose-Response Relationship, Radiation; D020450:Heavy Ions; D006801:Humans; D018499:Linear Energy Transfer; D008214:Lymphocytes / Q000166:cytology / Q000528:radiation effects*; D008297:Male; D009356:Neon; D010315:Particle Accelerators; D011829:Radiation Dosage; D012062:Relative Biological Effectiveness
chemical_list: D009356:Neon; D002244:Carbon