title: Potential targets for skeletal muscle impairment by hypogravity: basic characterization of resting ionic conductances and mechanical threshold of rat fast- and slow-twitch muscle fibers.
abstract: Prolonged hypogravity such as during space flights affects skeletal muscle function by inducing postural changes as well as reduced muscle strength and locomotion capacity. Also in rats, space flight as well as useful models of groundbased hypogravity induce marked atrophy in the slow-twitch soleus (SOL) muscle as opposed to slight or none in the fast-twitch ones such as extensor digitorum longus (EDL). Biochemical and histological studies on hindlimb suspended animals, showed a hypogravity-induced impairment of muscle function involving the transition of slow-twitch muscle type, responsible for postural control, toward the fast-twitch phenotype by modification of excitation-contraction pattern. In slow muscles of rats, hindlimb suspension induced upregulation of the fast isoform of myosin heavy-chain and increased expression of fast Ca2+ pump mRNA and protein, which is consistent with the increased Ca(2+)-dependent ATPase activity and the speeding of muscle relaxation, typical of fast muscles. Little is known about the modifications induced by hypogravity in the sarcolemmal ion channels function, which controls the pattern of muscle excitability and contractility. The normally high resting chloride conductance, which is required for the electrical stabilization of mammalian muscle fibers, may be a target of hypogravity modifications since a pharmacological block of this parameter determines, though an increase of excitability, the transition of the fast-twitch muscle phenotype toward the slow one either in adult or in developing rats. Hypogravity also induced increased expression of dihydropyridine receptors in soleus muscle, that are normally lower than that found in the fast ones. In this study, we characterized the electrical and contractile properties of rat extensor digitorum longus (EDL) and slow-twitch soleus SOL muscles fibers at the aim to better understand the molecular mechanisms leading to fiber transition.
authors.affiliation: Unit of Pharmacology, Department of Pharmacobiology, University of Bari, Italy.
authors.forename: A
authors.initials: A
authors.lastname: De Luca
authors.forename: A
authors.initials: A
authors.lastname: Liantonio
authors.forename: S
authors.initials: S
authors.lastname: Pierno
authors.forename: J F
authors.initials: JF
authors.lastname: Desaphy
authors.forename: C
authors.initials: C
authors.lastname: Leoty
authors.forename: D
authors.initials: D
authors.lastname: Conte Camerino
mesh_terms: D000818:Animals; D018118:Chloride Channels / Q000502:physiology*; D004594:Electrophysiology; D008297:Male; D008564:Membrane Potentials; D008839:Microelectrodes; D009119:Muscle Contraction / Q000502:physiology; D018656:Muscle Fibers, Fast-Twitch / Q000502:physiology*; D018657:Muscle Fibers, Slow-Twitch / Q000502:physiology*; D018482:Muscle, Skeletal / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D013026:Space Flight; D014893:Weightlessness
chemical_list: D018118:Chloride Channels