Gravity effects on connective tissue biosynthesis by cultured mesenchymal cells.
Quantitative and qualitative aspects of collagen synthesis under microgravity, normal gravity and hypergravity conditions were investigated during the spacelab D-2 mission by incubating human fibroblast cultures with [3H]-proline for 0, 4, 7, 10 and 20 hours. Quantitative analysis revealed an increase of collagen synthesis under microgravity conditions, being 40% higher than 1g controls. Hypergravity samples at 1.44g, 6.6g and 10g showed a decrease in collagen synthesis with increasing g, being down to about 15% at 10g. The relative proportion of collagen from total protein synthesized, the secretion of collagen by the cells, proline hydroxylation of individual collagen alpha-chains and the relative proportions of collagens I, III and V synthesized were not affected at any of the applied conditions.
Seitzer
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Institut fur Medizinische Molekularbiologie, Medizinische Universitat zu Lubeck, Germany.
Bodo
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Muller
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PK
D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis*; D005347:Fibroblasts; D006801:Humans; D006909:Hydroxyproline / Q000378:metabolism*; D018471:Hypergravity; D011392:Proline / Q000378:metabolism; D012867:Skin / Q000166:cytology; D013026:Space Flight; D013997:Time Factors; D014893:Weightlessness
D003094:Collagen; D011392:Proline; D006909:Hydroxyproline