Analysis of terpenelactones in Ginkgo biloba by high performance liquid chromatography and evaporative light scattering detection.
A reversed phase HPLC method permitting the determination of 5 terpenelactones in Ginkgo biloba, without the need of any sample preparation is presented in this paper. The compounds were successfully separated within 25 min by using a C-12 column, an evaporative light scattering (ELS) detector and a mobile phase comprising of ammonium acetate buffer, methanol and isobutanol. All terpenelactones were detectable at concentrations as low as 20.3 microg/ml. The analysis of G. biloba market products showed remarkable variations in the lactone content, and more than 2 fold differences in the suggested daily doses of the total lactones, from 8.84 mg to 18.28 mg, respectively.
Ganzera
M
M
National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, The University of Mississippi, University 38677, USA.
Zhao
J
J
Khan
I A
IA
D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D020441:Ginkgo biloba / Q000737:chemistry*; D007783:Lactones / Q000032:analysis*; D008027:Light; D013058:Mass Spectrometry; D008968:Molecular Conformation; D012542:Scattering, Radiation; D013056:Spectrophotometry, Ultraviolet; D013729:Terpenes / Q000032:analysis*
D007783:Lactones; D013729:Terpenes