Atmospheric pressure chemical ionization of fluorinated phenols in atmospheric pressure chemical ionization mass spectrometry, tandem mass spectrometry, and ion mobility spectrometry.
Atmospheric pressure chemical ionization (APCI)-mass spectrometry (MS) for fluorinated phenols (C6H5-xFxOH Where x = 0-5) in nitrogen with Cl- as the reagent ion yielded product ions of M Cl- through ion associations or (M-H)- through proton abstractions. Proton abstraction was controllable by potentials on the orifice and first lens, suggesting that some proton abstraction occurs through collision induced dissociation (CID) in the interface region. This was proven using CID of adduct ions (M Cl-) with Q2 studies where adduct ions were dissociated to Cl- or proton abstracted to (M-H)-. The extent of proton abstraction depended upon ion energy and structure in order of calculated acidities: pentafluorophenol > tetrafluorophenol > trifluorophenol > difluorophenol. Little or no proton abstraction occurred for fluorophenol, phenol, or benzyl alcohol analogs. Ion mobility spectrometry was used to determine if proton abstraction reactions passed through an adduct intermediate with thermalized ions and mobility spectra for all chemicals were obtained from 25 to 200 degrees C. Proton abstraction from M Cl- was not observed at any temperature for phenol, monofluorophenol, or difluorophenol. Mobility spectra for trifluorophenol revealed the kinetic transformations to (M-H)- either from M Cl- or from M2 Cl- directly. Proton abstraction was the predominant reaction for tetra- and penta-fluorophenols. Consequently, the evidence suggests that proton abstraction occurs from an adduct ion where the reaction barrier is reduced with increasing acidity of the O-H bond in C6H5-xFxOH.
Eiceman
G A
GA
Department of Chemistry and Biochemistry, New Mexico State University, Las Cruces, USA. geiceman@nmsu.edu
Bergloff
J F
JF
Rodriguez
J E
JE
Munro
W
W
Karpas
Z
Z
D001274:Atmospheric Pressure; D001592:Benzyl Alcohols / Q000032:analysis / Q000737:chemistry; D002713:Chlorine / Q000737:chemistry; D005461:Fluorine / Q000032:analysis* / Q000737:chemistry; D005464:Fluorobenzenes / Q000032:analysis / Q000737:chemistry; D007477:Ions; D013058:Mass Spectrometry / Q000379:methods*; D009584:Nitrogen / Q000737:chemistry; D010636:Phenols / Q000032:analysis / Q000737:chemistry; D011522:Protons; D005466:Fluorocarbons
D001592:Benzyl Alcohols; D002713:Chlorine; D005461:Fluorine; D005464:Fluorobenzenes; D007477:Ions; D009584:Nitrogen; D010636:Phenols; D011522:Protons; C106880:4-fluorophenol; C085539:2,3,4,5,6-pentafluorophenol; D005466:Fluorocarbons; C045453:2,3,5,6-tetrafluorophenol