[In vitro bovine and human lens epithelial cell culture studies on inhibition of posterior capsule opacification using a cyclic RGD peptide].
BACKGROUND
RGD peptides competitively inihibit adhesion molecules of the lens epithelial cells (LEC). The purpose of our study was to investigate whether this peptide is capable of detaching adherent cells and preventing posterior capsule opacification (PCO).


METHODS
Cultures of bovine and human LECs on culture dishes and discs of bovine anterior lens capsules were used. The inhibition of adhesion and the detachment of confluent LEC layers by the cyclic RGD peptide cRGDdFV were studied (incubation time was 3 days and 1 h and concentrations of 10(-4) and 10(-3) M were used).


RESULTS
A dose-dependent inhibition of adhesion (48% and 42%, respectively) was obtained. There was a significant difference between the control peptide group and cRGDdFV (p < 0.0001). Cell detachment from lens capsules was not achieved but complete detachment from the culture dish occurred after 37 min.


CONCLUSIONS
Short-term incubation of LECs by cRGDdFV did not lead to a sufficient inhibition of adhesion in vitro. A detachment of adherent LECs by cRGDdFV was not achieved.
Kojetinsky
C
C
Klinik und Poliklinik für Augenheilkunde, Charité Berlin, Campus Virchow-Klinikum, Augustenburger Platz 1, 13353 Berlin. corinakojetinsky@hotmail.com
Baatz
H
H
Pleyer
U
U
Hartmann
C
C
Rieck
P
P
D000268:Adhesiveness / Q000187:drug effects; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D002386:Cataract / Q000517:prevention & control*; D002417:Cattle; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000187:drug effects; D006801:Humans; D007908:Lens, Crystalline / Q000166:cytology* / Q000187:drug effects; D009842:Oligopeptides / Q000494:pharmacology*; D013997:Time Factors
D000970:Antineoplastic Agents; D009842:Oligopeptides; C047981:arginyl-glycyl-aspartic acid