title: The effect of changing the hydrophobic S1' subsite of thermolysin-like proteases on substrate specificity.
abstract: The hydrophobic S1' subsite is one of the major determinants of the substrate specificity of thermolysin and related M4 family proteases. In the thermolysin-like protease (TLP) produced by Bacillus stearothermophilus (TLP-ste), the hydrophobic S1' subsite is mainly formed by Phe130, Phe133, Val139 and Leu202. In the present study, we have examined the effects of replacing Leu202 by smaller (Gly, Ala, Val) and larger (Phe, Tyr) hydrophobic residues. The mutational effects showed that the wild-type S1' pocket is optimal for binding leucine side chains. Reduction of the size of residue 202 resulted in a higher efficiency towards substrates with Phe in the P1' position. Rather unexpectedly, the Leu202-->Phe and Leu202-->Tyr mutations, which were expected to decrease the size of the S1' subsite, resulted in a large increase in activity towards dipeptide substrates with Phe in the P1' position. This is probably due to the fact that 202Phe and 202Tyr adopt a second possible rotamer that opens up the subsite compared to Leu202, and also favours interactions with the substrate. To validate these results, we constructed variants of thermolysin with changes in the S1' subsite. Thermolysin and TLP-ste variants with identical S1' subsites were highly similar in terms of their preference for Phe vs. Leu in the P1' position.
authors.affiliation: Department of Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kerklaan, NN Haren, The Netherlands.
authors.forename: A
authors.initials: A
authors.lastname: de Kreij
authors.forename: B
authors.initials: B
authors.lastname: van den Burg
authors.forename: O R
authors.initials: OR
authors.lastname: Veltman
authors.forename: G
authors.initials: G
authors.lastname: Vriend
authors.forename: G
authors.initials: G
authors.lastname: Venema
authors.forename: V G
authors.initials: VG
authors.lastname: Eijsink
mesh_terms: D019943:Amino Acid Substitution / Q000235:genetics*; D001665:Binding Sites; D004151:Dipeptides / Q000737:chemistry / Q000378:metabolism; D001411:Geobacillus stearothermophilus / Q000201:enzymology* / Q000235:genetics; D007700:Kinetics; D008666:Metalloendopeptidases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D008958:Models, Molecular; D009154:Mutation / Q000235:genetics; D011487:Protein Conformation; D015203:Reproducibility of Results; D013379:Substrate Specificity; D013816:Thermodynamics; D013820:Thermolysin / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D004151:Dipeptides; D008666:Metalloendopeptidases; C044194:microbial metalloproteinases; D013820:Thermolysin