The diageotropica mutant of tomato lacks high specific activity auxin binding sites.
Tomato plants homozygous for the diageotropica (dgt) mutation exhibit morphological and physiological abnormalities which suggest that they are unable to respond to the plant growth hormone auxin (indole-3-acetic acid).  The photoaffinity auxin analog [3H]5N3-IAA specifically labels a polypeptide doublet of 40 and 42 kilodaltons in membrane preparations from stems of the parental variety, VFN8, but not from stems of plants containing the dgt mutation.  In roots of the mutant plants, however, labeling is indistinguishable from that in VFN8.  These data suggest that the two polypeptides are part of a physiologically important auxin receptor system, which is altered in a tissue-specific manner in the mutant.
Hicks
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GR
Department of Botany and Plant Pathology, Oregon State University, Corvallis 97331-2902.
Rayle
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DL
Lomax
T L
TL
D000345:Affinity Labels; D001386:Azides / Q000378:metabolism*; D001665:Binding Sites; D018546:Hypocotyl / Q000166:cytology / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure; D007210:Indoleacetic Acids / Q000032:analysis* / Q000378:metabolism*; D007425:Intracellular Membranes / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure; D018551:Solanum lycopersicum / Q000166:cytology / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure; D008861:Microsomes / Q000648:ultrastructure; D009154:Mutation; D010782:Photolysis; D010937:Plant Growth Regulators; D010940:Plant Proteins; D018517:Plant Roots / Q000166:cytology / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure; D011956:Receptors, Cell Surface / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D013997:Time Factors
D000345:Affinity Labels; D001386:Azides; D007210:Indoleacetic Acids; D010937:Plant Growth Regulators; D010940:Plant Proteins; D011956:Receptors, Cell Surface; C046131:auxin receptor, plant; C030737:indoleacetic acid; C068520:5-azido-1H-indole-3-acetic acid