Transformation from block-type to graft-type oligonucleotide-glycopolymer conjugates by self-organization with half-sliding complementary oligonucleotides and their lectin recognition.
Block-type oligonucleotide-glycopolymer conjugates bearing alpha-mannosides and beta-galactosides were prepared by coupling 5'-thiol-modified oligonucleotides with iodoacetamidated glycopolymers that were synthesized by telomerization. The conjugates minimally affected the DNA conformation and melting behavior of the duplex. Their self-organization via hybridization with the half-sliding complementary oligonucleotides produced graft-type conjugates or macromolecular gapped DNA duplexes grafted with glycopolymers at regular intervals, which was confirmed using size exclusion chromatography and electrophoresis. The binding affinity of block-type and self-organized graft-type conjugates to lectins was investigated using fluorometry. The affinity of the graft-type duplex assembly bearing mannosides to Con A was approximately 2 times stronger than that of block-type single-stranded or double-stranded conjugates with full complementary oligonucleotides. The organization strategy of DNA-glycopolymer conjugates might be useful for constructing novel glyco-clusters and also for developing a new methodology for gene therapy.
Akasaka
T
T
Department of Molecular Design, Graduate School of Engineering, Nagoya University, Chikusa, Nagoya 464-8603, Japan.
Matsuura
K
K
Kobayashi
K
K
D037102:Lectins / Q000378:metabolism*; D009690:Nucleic Acid Conformation / Q000187:drug effects; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization / Q000187:drug effects*; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D011485:Protein Binding; D013057:Spectrum Analysis; D013696:Temperature; D013873:Thionucleotides
D037102:Lectins; D009838:Oligodeoxyribonucleotides; D011134:Polysaccharides; D013873:Thionucleotides