Wuchereria bancrofti: detection of microfilariae in asymptomatic microfilaremic individuals with Setaria digitata antigens.
A dot-ELISA for detection of microfilariae of Wuchereria bancrofti in an endemic area was developed. This test can differentiate the endemic normals from the microfilaraemic asymptomatic individuals. Antigens of molecular weight 130 and 52 kDa of the cattle filaria worm Setaria digitata were used for this test. It was observed that these two antigens were also present in the serum of asymptomatic microfilaraemic individuals.
Wickremanayake
M N
MN
Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Colombo, Sri Lanka.
Ekanayake
S
S
Karunanayake
E H
EH
D000818:Animals; D000947:Antigens, Helminth; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005368:Filariasis / Q000175:diagnosis*; D006801:Humans; D017182:Setaria Nematode / Q000276:immunology*; D013188:Sri Lanka; D014958:Wuchereria bancrofti / Q000302:isolation & purification*
D000947:Antigens, Helminth