title: 5-Ethyl-2-mercaptothiazole as matrix for matrix-assisted laser desorption/ionization of a broad spectrum of analytes in positive and negative ion mode.
abstract: A preliminary investigation of the use of 5-ethyl-2-mercaptothiazole as matrix in matrix-assisted laser desorption/ionization (MALDI) of a broad spectrum of analytes is reported. The analytes studied are substance P, insulin, beta-cyclodextrin, triacylglycerols of coconut oil and polypropylene glycol 2000 (PPG 2000). In the positive ion mass spectra of the matrix/analyte combinations, the formation of [M + H]+ and [M + cation]+ species were observed and compared with those obtained by using well-established matrices such as alpha-cyano-4-hydroxycinnamic acid, genticic acid, sinapinic acid and dithranol. In addition, the usefulness of this new matrix for MALDI in negative ion mode is also described using substance P and beta-cyclodextrin as examples.
authors.affiliation: National Centre for Mass Spectrometry, Indian Institute of Chemical Technology, Hyderabad-500 007, India.
authors.forename: N P
authors.initials: NP
authors.lastname: Raju
authors.forename: S P
authors.initials: SP
authors.lastname: Mirza
authors.forename: M
authors.initials: M
authors.lastname: Vairamani
authors.forename: A R
authors.initials: AR
authors.lastname: Ramulu
authors.forename: M
authors.initials: M
authors.lastname: Pardhasaradhi
mesh_terms: D000074263:Coconut Oil; D003505:Cyclodextrins / Q000737:chemistry*; D007202:Indicators and Reagents; D007328:Insulin / Q000737:chemistry*; D010938:Plant Oils / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013373:Substance P / Q000737:chemistry*; D013438:Sulfhydryl Compounds; D013844:Thiazoles; D014280:Triglycerides / Q000737:chemistry*; D047392:beta-Cyclodextrins
chemical_list: C438896:5-ethyl-2-mercaptothiazole; D003505:Cyclodextrins; D007202:Indicators and Reagents; D007328:Insulin; D010938:Plant Oils; D013438:Sulfhydryl Compounds; D013844:Thiazoles; D014280:Triglycerides; D047392:beta-Cyclodextrins; D013373:Substance P; C031215:betadex; D000074263:Coconut Oil