Antioxidant properties of alpha-crystallin.
alpha-Crystallin is a major chaperone lens protein to which has been ascribed antioxidant functions. In the present work we have evaluated the antioxidant and free radical scavenging properties of bovine alpha-crystallin in a series of in vitro models: zimosan-induced, luminol-enhanced chemiluminescence response of polymorphonuclear leukocytes, the autoxidation of brain homogenate, bleaching of 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)-derived radical cations, trapping of peroxyl radicals, and reactivity toward hypochloric acid. In all these systems, the reactivity of alpha-crystallin is higher than or similar to that of bovine serum albumin. It is concluded that, given the high concentrations of ol-crystallin in the lenses, its capacity to interact with free radicals and to remove hypochlorous acid could contribute to the maintenance of the lens functionality.
Manzanares
D
D
Centro de Investigaciones Biomédicas, Instituto Superior de Ciencias Médicas de la Habana, Cuba.
Bauby
C
C
de la Peña
R
R
Garcia
J C
JC
Sanchez
R
R
Martinez
S
S
Romay
C H
CH
López-Reconde
J L
JL
Pino
E
E
Lissi
E A
EA
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D001921:Brain / Q000201:enzymology / Q000378:metabolism; D002417:Cattle; D003459:Crystallins / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D005609:Free Radicals; D006801:Humans; D006997:Hypochlorous Acid / Q000378:metabolism; D007908:Lens, Crystalline / Q000737:chemistry; D008163:Luminescent Measurements; D009504:Neutrophils / Q000737:chemistry / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D010084:Oxidation-Reduction; D010545:Peroxides / Q000378:metabolism; D010798:Phycocyanin / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D015054:Zymosan / Q000276:immunology / Q000494:pharmacology
D000975:Antioxidants; D003459:Crystallins; D005609:Free Radicals; D010545:Peroxides; D017392:Thiobarbituric Acid Reactive Substances; D010798:Phycocyanin; C049375:perhydroxyl radical; D006997:Hypochlorous Acid; D015054:Zymosan