Mannose binding lectin and FcgammaRIIa (CD32) polymorphism in Spanish systemic lupus erythematosus patients.
OBJECTIVE
Mannose binding lectin (MBL) and FcgammaRII (CD32) polymorphisms have both been implicated as candidate susceptibility genes in systemic lupus erythematosus (SLE). The aim of this study was to evaluate the relationship of these polymorphisms with SLE.


METHODS
We studied a cohort of 125 SLE patients from Barcelona, Spain and 138 geographically matched controls. Sequence-specific primer-polymerase chain reaction (SSP-PCR) amplification was used to determine CD32 and MBL structural polymorphisms. MBL haplotypes were established using sequence-specific oligonucleotide probing techniques.


RESULTS
Patients carried the MBL codon 54 mutant allele more frequently than controls [odds ratio (OR) 2.2; 95% confidence interval (CI) 1.2-4.0; P=0.007] and the haplotype HY W52 W54 W57 was found to be significantly lower in cases compared with controls (OR 0.6; 95% CI 0.4-0.9; P=0.016).


CONCLUSIONS
The MBL gene codon 54 mutant allele appears to be a risk factor for SLE, whilst haplotypes encoding for high levels of MBL are protective against the disease. Differences between controls and patients were not significant when considering the FcgammaRIIa polymorphisms; similar results were observed for renal affectation.
Villarreal
J
J
Hospital General Vall d'Hebron, Passieg Vall d'Hebron 119-129, Barcelona, Spain.
Crosdale
D
D
Ollier
W
W
Hajeer
A
A
Thomson
W
W
Ordi
J
J
Balada
E
E
Villardell
M
M
Teh
L S
LS
Poulton
K
K
D000209:Acute-Phase Proteins / Q000235:genetics* / Q000378:metabolism; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D004247:DNA / Q000032:analysis; D005787:Gene Frequency; D006239:Haplotypes; D006801:Humans; D037102:Lectins / Q000235:genetics / Q000378:metabolism; D008178:Lupus Erythematosus, Cutaneous / Q000235:genetics / Q000378:metabolism; D008180:Lupus Erythematosus, Systemic / Q000150:complications / Q000235:genetics* / Q000378:metabolism; D008181:Lupus Nephritis / Q000235:genetics / Q000378:metabolism; D020945:Lupus Vasculitis, Central Nervous System / Q000235:genetics / Q000378:metabolism; D037241:Mannose-Binding Lectins; D009154:Mutation; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D017452:Receptors, IgG / Q000235:genetics* / Q000378:metabolism; D013030:Spain
D000209:Acute-Phase Proteins; D002352:Carrier Proteins; D037102:Lectins; D037241:Mannose-Binding Lectins; D017452:Receptors, IgG; D004247:DNA