High-performance liquid chromatographic enantioseparation of beta-amino acids.
Direct and indirect high-performance liquid chromatographic methods were developed for the enantioseparation of beta-amino acids (beta-substituted-beta-alanines). Direct separation involved the application of chiral columns: Crownpak CR(+), Chirobiotic T and Chirobiotic R. Indirect separation was based on precolumn derivatization with 2,3,4,6-tetra-O-acetyl-beta-D-glucopyranosyl isothiocyanate or N-alpha-(2,4-dinitro-5-fluorophenyl)-L-alanineamide (Marfey's reagent), with subsequent separation on an achiral column. The chromatographic conditions were varied to achieve optimum separation.
Péter
A
A
Department of Inorganic and Analytical Chemistry, University of Szeged, Hungary. apeter@chem.u-szeged.hu
Lázár
L
L
Fülöp
F
F
Armstron
D W
DW
D000596:Amino Acids / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D013237:Stereoisomerism
D000596:Amino Acids