title: Recombinant bacterial cells as efficient biocatalysts for the production of nucleosides.
abstract: The preparation of nucleosides as well as their base-modified analogues using purified nucleoside phosphorylase enzymes or, more conveniently, using whole bacterial cells is described. The development of genetically modified strains of Escherichia coli, able to over-produce Uridine-phosphorylase and Purine-nucleoside-phosphorylase in the same cells, improves the specific biocatalytic activity and the consequent industrial scale approach.
authors.affiliation: Dipartimento di Genetica e Biologia dei Microorganismi, University of Milan, Via Celoria 26, 20133 Milan, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Spoldi
authors.forename: D
authors.initials: D
authors.lastname: Ghisotti
authors.forename: S
authors.initials: S
authors.lastname: Calì
authors.forename: M
authors.initials: M
authors.lastname: Grisa
authors.forename: G
authors.initials: G
authors.lastname: Orsini
authors.forename: G
authors.initials: G
authors.lastname: Tonon
authors.forename: G
authors.initials: G
authors.lastname: Zuffi
mesh_terms: D004926:Escherichia coli / Q000201:enzymology / Q000378:metabolism*; D009705:Nucleosides / Q000096:biosynthesis*; D011683:Purine-Nucleoside Phosphorylase / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D014543:Uridine Phosphorylase / Q000378:metabolism*; D014740:Vidarabine / Q000096:biosynthesis
chemical_list: D009705:Nucleosides; D011994:Recombinant Proteins; D011683:Purine-Nucleoside Phosphorylase; D014543:Uridine Phosphorylase; D014740:Vidarabine