title: An efficient screening approach for anti-Microcystis compounds based on knowledge of aquatic microbial ecosystem.
abstract: To improve the efficiency of screening for anti-Microcystis compounds, we planned to use algae-lysing bacteria that kill the organisms of water blooms. A two step-screening process was carried out, i.e., the screening of algae-lysing bacteria and the selection of anti-Microcystis producers from the bacteria. Sources for the isolation of the bacteria were a co-cultivated fluid of a water sample with axenic Microcystis viridis, a water sample collected in a water bloom season, and a water bloom sample. The water bloom sample was the best source for the isolation of the algae-lysing bacteria and such bacteria were shown to exhibit potent activity. Seventeen strains out of 20 isolated algae-lysing bacteria produced anti-Microcystis activities, and one of the principles was the previously reported argimicin A. These results indicate that algae-lysing bacteria in water blooms may be good sources for potent and selective anticyanobacterial compounds.
authors.affiliation: Department of Bioscience and Biotechnology, Faculty of Science and Engineering, Ritsumeikan University, Kusatsu, Shiga, Japan. imamura@se.ritsumei.ac.jp
authors.forename: N
authors.initials: N
authors.lastname: Imamura
authors.forename: I
authors.initials: I
authors.lastname: Motoike
authors.forename: N
authors.initials: N
authors.lastname: Shimada
authors.forename: M
authors.initials: M
authors.lastname: Nishikori
authors.forename: H
authors.initials: H
authors.lastname: Morisaki
authors.forename: H
authors.initials: H
authors.lastname: Fukami
mesh_terms: D002457:Cell Extracts / Q000494:pharmacology; D004269:DNA, Bacterial / Q000032:analysis; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D056890:Eukaryota / Q000382:microbiology*; D046931:Microcystis / Q000187:drug effects; D010572:Pest Control, Biological / Q000379:methods; D010802:Phylogeny; D016958:Pseudomonas putida / Q000737:chemistry / Q000145:classification* / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000145:classification; D014871:Water Microbiology
chemical_list: D002457:Cell Extracts; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S