Nuclear DNA content and base composition in 28 taxa of Musa.
The nuclear DNA content of 28 taxa of Musa was assessed by flow cytometry, using line PxPC6 of Petunia hybrida as an internal standard. The 2C DNA value of Musa balbisiana (BB genome) was 1.16 pg, whereas Musa acuminata (AA genome) had an average 2C DNA value of 1.27 pg, with a difference of 11% between its subspecies. The two haploid (IC) genomes, A and B, comprising most of the edible bananas, are therefore of similar size, 0.63 pg (610 million bp) and 0.58 pg (560 million bp), respectively. The genome of diploid Musa is thus threefold that of Arabidopsis thaliana. The genome sizes in a set of triploid Musa cultivars or clones were quite different, with 2C DNA values ranging from 1.61 to 2.23 pg. Likewise, the genome sizes of tetraploid cultivars ranged from 1.94 to 2.37 pg (2C). Apparently, tetraploids (for instance, accession I.C.2) can have a genome size that falls within the range of triploid genome sizes, and vice versa (as in the case of accession Simili Radjah). The 2C values estimated for organs such as leaf, leaf sheath, rhizome, and flower were consistent, whereas root material gave atypical results, owing to browning. The genomic base composition of these Musa taxa had a median value of 40.8% GC (SD = 0.43%).
Kamaté
K
K
Institut des Sciences du Vegetal, Centre National de la Recherche Scientifique, UPR2355, Gif-sur-Yvette, France.
Brown
S
S
Durand
P
P
Bureau
J M
JM
De Nay
D
D
Trinh
T H
TH
D000465:Algorithms; D002467:Cell Nucleus / Q000235:genetics*; D004247:DNA; D005434:Flow Cytometry; D018745:Genome, Plant; D028521:Musa; D011003:Ploidies; D013045:Species Specificity
D004247:DNA