Immunological and biochemical evidence for nuclear localization of annexin in peas.
Immunofluorescent localization of annexins using an anti-pea annexin polyclonal antibody (anti-p35) in pea (Pisum sativum) leaf and stem epidermal peels showed staining of the nuclei and the cell periphery. Nuclear staining was also seen in cell teases prepared from pea plumules. The amount of nuclear stain was reduced both by fixation time and by dehydration and organic solvent treatment. Observation with confocal microscopy demonstrated that the anti-p35 stain was diffusely distributed throughout the nuclear structure. Immunoblots of purified nuclei, nuclear envelope matrix, nucleolar, and chromatin fractions showed a cross-reactive protein band of 35 kDa. These data are the first to show annexins localized in plant cell nuclei where they may play a role in nuclear function.
Clark
G B
GB
Department of Botany, University of Texas, Austin 78713, USA.
Dauwalder
M
M
Roux
S J
SJ
D017302:Annexins / Q000032:analysis* / Q000378:metabolism; D015153:Blotting, Western; D002467:Cell Nucleus / Q000737:chemistry* / Q000378:metabolism; D007150:Immunohistochemistry / Q000379:methods; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D018532:Pisum sativum / Q000737:chemistry* / Q000166:cytology* / Q000378:metabolism; D019441:Plant Epidermis / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism; D018515:Plant Leaves / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D010940:Plant Proteins / Q000032:analysis / Q000378:metabolism; D016707:Tissue Fixation
D017302:Annexins; D010940:Plant Proteins