High resolution immunophenotypic analysis of chronic lymphocytic leukemic cells by enzymatic amplification staining.
Immunophenotypic analysis of chronic lymphocytic leukemia (CLL) cells is essential for the diagnosis of this disorder. Unfortunately, surface immunoglobulin light chains and CD79b are expressed faintly or not at all by CLL cells from many patients. We developed an enzymatic amplification staining procedure that amplifies the fluorescent signal by 10- to 100-fold. By using this technology, we have been able to resolve immunoglobulin light chain exclusion and CD79b expression on the cells from most cases. This new capability can be used for high-resolution immunophenotypic analysis of leukemias and lymphomas.
Kaplan
D
D
Dept of Pathology, Case Western Reserve University, Biomedical Research Bldg, Room 926, 2109 Adelbert Rd, Cleveland, OH 44106-4943, USA.
Meyerson
H
H
Lewandowska
K
K
D015703:Antigens, CD / Q000032:analysis; D051925:CD79 Antigens; D017248:Enzyme Multiplied Immunoassay Technique; D005188:False Negative Reactions; D005434:Flow Cytometry; D006801:Humans; D007147:Immunoglobulin Light Chains / Q000032:analysis; D016130:Immunophenotyping / Q000379:methods*; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000276:immunology* / Q000473:pathology; D012680:Sensitivity and Specificity
D015703:Antigens, CD; D051925:CD79 Antigens; C497883:CD79B protein, human; D007147:Immunoglobulin Light Chains