Effect of acetophenone derived Mannich bases on cellular glutathione level in Jurkat cells. A possible mechanism of action.
The effect of the acetophenone derived mono Mannich bases 1-3 and bis Mannich base 7 (bis derivative of compound 3) on cellular glutathione level was investigated in Jurkat cells. The cells were exposed to the compounds in phosphate buffered saline for 1 h in 37 degrees C with gentle shaking and then glutathione level was measured. Especially, mono Mannich base 3 and its bis derivative 7 decreased total glutathione level in a dose-dependent manner. The results provide further support for the thiol alkylation mechanism explaining the cytotoxic activity of Mannich bases.
Gul
M
M
Department of Physiology, University of Kuopio, Kuopio, Finland.
Gul
H I
HI
Vepsalainen
J
J
Erciyas
E
E
Hanninen
O
O
D000098:Acetophenones / Q000737:chemistry*; D000478:Alkylation; D005978:Glutathione / Q000378:metabolism*; D006801:Humans; D019169:Jurkat Cells; D008352:Mannich Bases / Q000737:chemistry*
D000098:Acetophenones; D008352:Mannich Bases; D005978:Glutathione