Replication of the rat aldolase B locus differs between aldolase B-expressing and non-expressing cells.
We previously reported a rat chromosomal origin of DNA replication (oriA1) that encompassed the aldolase B (AldB) gene promoter. Here, we examined utilization of oriA1 in AldB-expressing and non-expressing cells. The results suggested the occurrence of mutually exclusive regulation between DNA replication and transcription. Nascent strand abundance as assayed by competitive polymerase chain reaction using bromodeoxyuridine-labeled nascent DNA indicated that oriA1 is not utilized in AldB-expressing cells, while it is fired in non-expressing cells. In the latter non-expressing cells, the replication fork seemed to slow at 20-22 kb downstream of oriA1.
Miyagi
S
S
Cryobiosystem Research Center, Faculty of Agriculture, Iwate University, Ueda, Morioka, Iwate 020-8550, Japan.
Zhao
Y P
YP
Saitoh
Y
Y
Tamai
K
K
Tsutsumi
K I
KI
D000818:Animals; D000964:Antimetabolites, Antineoplastic / Q000494:pharmacology; D001973:Bromodeoxyuridine / Q000494:pharmacology; D004261:DNA Replication; D005634:Fructose-Bisphosphate Aldolase / Q000096:biosynthesis* / Q000235:genetics*; D015151:Immunoblotting; D008957:Models, Genetic; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D051381:Rats; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
D000964:Antimetabolites, Antineoplastic; D005634:Fructose-Bisphosphate Aldolase; D001973:Bromodeoxyuridine