title: Temperature induces variations in the retinal cell proliferation rate in a cyprinid.
abstract: We quantitatively evaluate the changes of the proliferative cell populations in the adult tench retinas maintained at 6 degrees C and 20 degrees C by both PCNA antigen detection and flow cytometry-based DNA measurements. Both the overall percentage of S-phase cells in the whole retinas and the number of PCNA-positive cells in each of the retinal layers were significantly lower in the tench kept at 6 degrees C, indicating that temperature affects the retinal germinal cell proliferation.
authors.affiliation: Biología Celular, INCyL, Universidad de Salamanca, 37007, Salamanca, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Velasco
authors.forename: E
authors.initials: E
authors.lastname: Cid
authors.forename: J
authors.initials: J
authors.lastname: Ciudad
authors.forename: A
authors.initials: A
authors.lastname: Orfao
authors.forename: J
authors.initials: J
authors.lastname: Aijon
authors.forename: J M
authors.initials: JM
authors.lastname: Lara
mesh_terms: D000818:Animals; D001831:Body Temperature / Q000502:physiology*; D002454:Cell Differentiation / Q000502:physiology; D002455:Cell Division / Q000502:physiology*; D019070:Cell Lineage / Q000502:physiology; D048429:Cell Size / Q000502:physiology; D003530:Cyprinidae / Q000033:anatomy & histology / Q000254:growth & development* / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D005434:Flow Cytometry; D007150:Immunohistochemistry; D023421:Models, Animal; D009416:Nerve Regeneration / Q000502:physiology; D009457:Neuroglia / Q000166:cytology / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D010786:Photoreceptor Cells / Q000166:cytology / Q000378:metabolism; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D012160:Retina / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism; D012165:Retinal Ganglion Cells / Q000166:cytology / Q000378:metabolism; D013234:Stem Cells / Q000378:metabolism*
chemical_list: D018809:Proliferating Cell Nuclear Antigen; D004247:DNA