Estimating the number of complex particles using the ConnEulor principle.
An unbiased counting rule for the number of topologically simple objects of any shape, size and distribution in 3D space is a pertinent problem in stereology. Combining the disector principle with the object's 3D Euler number makes possible number estimation, which until now has been obtainable only by exhaustive serial sections. The disector is a set of two sections where the object's profiles in one section are compared with its profiles on the neighbouring section, and the number of new 2D topological events is recorded. In a disector of known volume the sum of topological events is a direct estimate of the disector contribution to the total Euler number, which forms the basis for an ultimate number estimator in 3D, the ConnEulor. The method is illustrated by an electron microscopic study of the number of mitochondria in the exocrine cells of the pancreas.
Kroustrup
J P
JP
Stereological Research Laboratory, Institute of Experimental Clinical Research, University Institute of Pathology and Medical Department M, University Hospital, Aarhus University, Aarhus, Denmark. kroustrup@dadlnet.dk
Gundersen
H J
HJ
D000818:Animals; D002467:Cell Nucleus / Q000648:ultrastructure; D004285:Dogs; D008854:Microscopy, Electron / Q000379:methods*; D008928:Mitochondria / Q000648:ultrastructure*; D010179:Pancreas / Q000166:cytology / Q000648:ultrastructure; D013223:Statistics as Topic / Q000379:methods