title: Using rapid freeze and freeze-substitution for the preparation of yeast cells for electron microscopy and three-dimensional analysis.
authors.affiliation: Department of Molecular, Cellular and Developmental Biology, Porter Biosciences, University of Colorado-Boulder, Boulder, Colorado 80309, USA.
authors.forename: T H
authors.initials: TH
authors.lastname: Giddings
authors.forename: E T
authors.initials: ET
authors.lastname: O'Toole
authors.forename: M
authors.initials: M
authors.lastname: Morphew
authors.forename: D N
authors.initials: DN
authors.lastname: Mastronarde
authors.forename: J R
authors.initials: JR
authors.lastname: McIntosh
authors.forename: M
authors.initials: M
authors.lastname: Winey
mesh_terms: D005615:Freezing; D008854:Microscopy, Electron / Q000379:methods*; D016253:Microscopy, Immunoelectron / Q000379:methods*; D008870:Microtubules / Q000648:ultrastructure; D008938:Mitosis; D011312:Pressure; D012568:Schizosaccharomyces / Q000378:metabolism / Q000648:ultrastructure; D008941:Spindle Apparatus / Q000648:ultrastructure*; D013997:Time Factors