Assessment of antioxidant activity of some antileprotic drugs.
DPPH (alpha,alpha-diphenyl-beta-picryl hydrazyl) (CAS 217-591-8), a stable free radical can be used to determine the antioxidant activity (AOA) of some drugs. In the present study the DPPH method was used for the first time to test AOA of dapsone (CAS 80-08-0), clofazimine (CAS 2030-63-9) and rifampicin (CAS 13282-42-1) in vitro and deproteinated blood method. Ascorbic acid (CAS 50-81-7) was used as a control in the study, which showed concentration-dependent antioxidant activity. Rifampicin showed a per se effect but it showed concentration dependent decrease in the DPPH absorbance. Ascorbic acid, dapsone and rifampicin showed DPPH scavenging activity both in vitro and deproteinated blood method. Clofazimine did not have any influence on DPPH. This method may be extended to different drugs for testing their AOA in biological fluids.
Kalpana
T
T
Drug Metabolism and Clinical Pharmacokinetics Division, University College of Pharmaceutical Sciences, Kakatiya University, Warangal, A.P., India.
Karunakar
N
N
Reddy
M S
MS
Prabhakar
M C
MC
Krishna
D R
DR
D000328:Adult; D000975:Antioxidants; D001205:Ascorbic Acid / Q000494:pharmacology; D015764:Bepridil / Q000031:analogs & derivatives* / Q000378:metabolism; D001713:Biphenyl Compounds; D002991:Clofazimine / Q000494:pharmacology; D003622:Dapsone / Q000494:pharmacology; D006801:Humans; D007917:Leprostatic Agents / Q000494:pharmacology*; D008297:Male; D010084:Oxidation-Reduction; D010851:Picrates; D012293:Rifampin / Q000494:pharmacology; D013056:Spectrophotometry, Ultraviolet
D000975:Antioxidants; D001713:Biphenyl Compounds; D007917:Leprostatic Agents; D010851:Picrates; D015764:Bepridil; D003622:Dapsone; D002991:Clofazimine; C004931:1,1-diphenyl-2-picrylhydrazyl; D001205:Ascorbic Acid; D012293:Rifampin