Interaction of LNA oligonucleotides with mdr1 promoter.
LNA oligonucleotides [1] can be used for targeting to double stranded DNA by the "strand invasion" mechanism. We used affinity modification by reactive oligonucleotide conjugates for investigation of oligonucleotides interaction with structured DNA. The tested LNAs and oligonucleotides of the same sequence were assayed as anti-mdr1 drugs in different cell cultures. One of the oligos, LNA79 strongly inhibited mdr1 induction in Hela cells and totally prevented activation of mdr1 in K-562.
Chernolovskaya
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EL
Novosibirsk Institute of Bioorganic Chemistry, Lavrentiev ave., 8, Novosibirsk, 630090, Russia. elena_ch@niboch.nsc.ru
Koshkin
A A
AA
Vlassov
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VV
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000037:antagonists & inhibitors* / Q000096:biosynthesis / Q000235:genetics; D004278:DNA, Superhelical / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D020014:K562 Cells; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000737:chemistry / Q000494:pharmacology*; D010957:Plasmids / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000187:drug effects
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D004278:DNA, Superhelical; D009841:Oligonucleotides