Oligomerization reactions of deoxyribonucleotides on montmorillonite clay:  the effect of mononucleotide structure on phosphodiester bond formation.
Adenine deoxynucleotides bind more strongly to Na(+)-montmorillonite than do the corresponding ribonucleotides.  Thymidine nucleotides binds less strongly to Na(+)-montmorillonite than do the corresponding adenine deoxynucleotides.  Oligomers of 2'-dpA up to the tetramer were detected in the reaction 2'-d-5'-AMP with EDAC (a water-soluble carbodiimide) in the presence of Na(+)-montmorillonite.  Reaction of 3'-d-5'-AMP with EDAC on Na(+)-montmorillonite yields 3'-d-2',5'-pApA while the reaction of 2'-d-3'-AMP yields almost exclusively 3',5'-cdAMP.  The reaction of 5'-TMP under the same reaction conditions give 3',5'-cpTpT and 3',5'-pTpT while 3'-TMP gives mainly 3',5'-cpT.  The yield of dinucleotide products (dpNpN) containing the phosphodiester bond is 1% or less when Na(+)-montmorillonite is omitted from the reaction mixture.
Ferris
J P
JP
Department of Chemistry, Rensselaer Polytechnic Institute, Troy, NY 12180-3590, USA.
Kamaluddin
D000538:Aluminum Silicates / Q000737:chemistry*; D001546:Bentonite / Q000737:chemistry*; D001704:Biopolymers; D002234:Carbodiimides / Q000737:chemistry; D002384:Catalysis; D000077215:Clay; D003854:Deoxyribonucleotides / Q000737:chemistry*; D019143:Evolution, Molecular; D008903:Minerals / Q000737:chemistry; D008956:Models, Chemical; D009711:Nucleotides / Q000737:chemistry*; D009841:Oligonucleotides / Q000138:chemical synthesis*; D000069497:Origin of Life; D012313:RNA / Q000138:chemical synthesis; D013698:Templates, Genetic
D000538:Aluminum Silicates; D001704:Biopolymers; D002234:Carbodiimides; D003854:Deoxyribonucleotides; D008903:Minerals; D009711:Nucleotides; D009841:Oligonucleotides; D001546:Bentonite; C074139:1-ethyl-3-(3-dimethylaminoethyl)carbodiimide; D012313:RNA; D000077215:Clay