Real time confocal laser scanning microscopy: potential applications in space medicine and cell biology.
Photodynamic therapy (PDT), in which tissues may be rendered fatally light-sensitive represents a relatively novel treatment for cancer and other disorders such as cardiovascular disease. It offers significant application to disease control in an isolated environment such as space flight. In studying PDT in the laboratory, low energy lasers such as HeNe lasers are used to activate the photosensitized cellular target. A major problem associated with these studies is that events occurring during actual exposure of the target cells to the system cannot be examined in real time. In this study HeLa cells were photosensitized and photodynamic activation was accomplished using the scanning microbeam from a confocal laser scanning microscope. This form of activation allowed for simultaneous photoactivation and observation and facilitated the recording of events at a microscopic level during photoactivation. Effects of photodynamic activation on the target cells were monitored using the fluorophores rhodamine 123 and ethidium homodimer-1. Potential applications of these forms of analyses to space medicine and cell biology are discussed.
Rollan
A
A
Biotechnology Research Group, University of Ulster, Londonderry, Northern Ireland.
Ward
T
T
McHale
A P
AP
D000337:Aerospace Medicine / Q000379:methods*; D002468:Cell Physiological Phenomena; D004996:Ethidium / Q000031:analogs & derivatives; D005456:Fluorescent Dyes; D006367:HeLa Cells; D006414:Hematoporphyrin Photoradiation / Q000379:methods*; D006801:Humans; D018613:Microscopy, Confocal / Q000379:methods*; D010778:Photochemotherapy; D020112:Rhodamine 123; D018581:Technology Transfer
D005456:Fluorescent Dyes; D020112:Rhodamine 123; C018533:ethidium homodimer; D004996:Ethidium