title: Synthesis of a new 5'-O-triphosphate analog of 5-methyl 2'-O-deoxycytidine. Preliminary in vitro labelling for non-radioactive detection of DNA.
abstract: We report the synthesis of the triphosphate of 5-methyl 4-N-[6-(p-bromobenzamido)hex-1-yl]-2'-O-deoxycytidine 3A. We also analyzed the formation of intramolecular H-bonds of 5-methyl 4-N-[n-[6-(p-bromobenzamido) caproyl amino]alk-1-yl]-2'-deoxycytidine compounds, and confirmed their presence by 1H-NMR studies. In vitro DNA labeling with modified nucleotides is preliminarily evaluated.
authors.affiliation: Organic Chemistry Department, National Center for Scientific Research, Havana, Cuba.
authors.forename: C
authors.initials: C
authors.lastname: Rodríguez-Tanty
authors.forename: R
authors.initials: R
authors.lastname: Pérez
authors.forename: J
authors.initials: J
authors.lastname: Miranda
authors.forename: H
authors.initials: H
authors.lastname: Vélez-Castro
authors.forename: A
authors.initials: A
authors.lastname: Rosado
authors.forename: A
authors.initials: A
authors.lastname: Macías
authors.forename: V
authors.initials: V
authors.lastname: Vidal-Alles
authors.forename: M J
authors.initials: MJ
authors.lastname: Araña
authors.forename: N
authors.initials: N
authors.lastname: Pérez Souto
authors.forename: D
authors.initials: D
authors.lastname: Higginson-Clarke
authors.forename: A M
authors.initials: AM
authors.lastname: Riverón
mesh_terms: D004247:DNA / Q000032:analysis* / Q000378:metabolism*; D003841:Deoxycytidine / Q000031:analogs & derivatives* / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism*; D004926:Escherichia coli; D006860:Hydrogen Bonding; D015151:Immunoblotting; D009682:Magnetic Resonance Spectroscopy; D010957:Plasmids / Q000378:metabolism; D016339:Spectrometry, Mass, Fast Atom Bombardment
chemical_list: D003841:Deoxycytidine; D004247:DNA; C016569:5-methyldeoxycytidine