title: Phenylalkyl backbone modified oligodeoxynucleotides, their synthesis and the influence of the alkyl chain length.
abstract: Phenylalkyl modified phosphoramidites (alkyl chain length n = 1, 2, 3, 5; Fig. 1) were synthesised and incorporated into a DNA hexamer (5'-d(GCCp-GCG); p = place of modification). The obtained diastereomeres were separated by RP-HPLC. After hybridisation with the complementary DNA strand Tm-value and thermodynamic data were measured. The stability of duplexes depends on the linker length and the absolute configuration of the backbone modified oligodeoxynucleotides (Rp, Sp).
authors.affiliation: Institut für Organische Chemie, Johann Wolfgang Goethe-Universität, Marie Curie Strasse 11, D-60439 Frankfurt am Main, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Amberg
authors.forename: J W
authors.initials: JW
authors.lastname: Engels
mesh_terms: D006841:Hydrocarbons, Aromatic / Q000138:chemical synthesis / Q000737:chemistry; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000737:chemistry*; D009943:Organophosphorus Compounds / Q000138:chemical synthesis / Q000737:chemistry; D013329:Structure-Activity Relationship
chemical_list: D006841:Hydrocarbons, Aromatic; D009841:Oligonucleotides; D009943:Organophosphorus Compounds; C434331:phosphoramidite