title: Alterations to penicillin-binding proteins 1A, 2B and 2X amongst penicillin-resistant clinical isolates of Streptococcus pneumoniae serotype 23F from the nasopharyngeal flora of children.
abstract: Various amino acid substitutions were identified in the three major penicillin-binding proteins (PBP1A, PBP2B and PBP2X) of eight clinical isolates of Streptococcus pneumoniae serotype 23F collected from children. The particular changes related to the level of penicillin resistance. Alterations were detected in an isolate with a penicillin MIC as low as 0.06 mg/L. These results confirm that the level of penicillin resistance in pneumococci reflects with sequential alterations of PBPs in clinical isolates.
authors.affiliation: Service de Microbiologie, CHU Necker-Enfants-Malades, 149 rue de Sèvres, 75015 Paris, France.
authors.forename: Agnes
authors.initials: A
authors.lastname: Ferroni
authors.affiliation: Service de Microbiologie, CHU Necker-Enfants-Malades, 149 rue de Sèvres, 75015 Paris, France.
authors.forename: Patrick
authors.initials: P
authors.lastname: Berche
mesh_terms: D000595:Amino Acid Sequence; D019881:Aminoacyltransferases; D001426:Bacterial Proteins; D001483:Base Sequence; D002352:Carrier Proteins / Q000737:chemistry* / Q000235:genetics; D002675:Child, Preschool; D016521:Electrophoresis, Gel, Pulsed-Field; D006602:Hexosyltransferases / Q000737:chemistry* / Q000235:genetics; D006801:Humans; D007223:Infant; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000737:chemistry* / Q000235:genetics; D002267:Muramoylpentapeptide Carboxypeptidase / Q000737:chemistry* / Q000235:genetics; D009305:Nasopharynx / Q000382:microbiology; D010403:Penicillin Resistance / Q000502:physiology; D046915:Penicillin-Binding Proteins; D010458:Peptidyl Transferases / Q000737:chemistry* / Q000235:genetics; D011008:Pneumococcal Infections / Q000382:microbiology*; D016415:Sequence Alignment; D013296:Streptococcus pneumoniae / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D009097:Multienzyme Complexes; D046915:Penicillin-Binding Proteins; C072223:PBP 2x protein, Streptococcus; D019881:Aminoacyltransferases; C478701:penicillin-binding protein 2b, Streptococcus; D010458:Peptidyl Transferases; D006602:Hexosyltransferases; D002267:Muramoylpentapeptide Carboxypeptidase