title: Isolated hypervariable regions derived from streptococcal M proteins specifically bind human C4b-binding protein: implications for antigenic variation.
abstract: Antigenic variation in microbial surface proteins represents an apparent paradox, because the variable region must retain an important function, while exhibiting extensive immunological variability. We studied this problem for a group of streptococcal M proteins in which the approximately 50-residue hypervariable regions (HVRs) show essentially no residue identity but nevertheless bind the same ligand, the human complement regulator C4b-binding protein (C4BP). Synthetic peptides derived from different HVRs were found to retain the ability to bind C4BP, implying that the HVR corresponds to a distinct ligand-binding domain that can be studied in isolated form. This finding allowed direct characterization of the ligand-binding properties of isolated HVRs and permitted comparisons between different HVRs in the absence of conserved parts of the M proteins. Affinity chromatography of human serum on immobilized peptides showed that they bound C4BP with high specificity and inhibition experiments indicated that different peptides bound to the same site in C4BP. Different C4BP-binding peptides did not exhibit any immunological cross-reactivity, but structural analysis suggested that they have similar folds. These data show that the HVR of streptococcal M protein can exhibit extreme variability in sequence and immunological properties while retaining a highly specific ligand-binding function.
authors.affiliation: Department of Medical Microbiology, Dermatology, and Infection, Lund University, Sölvegatan 23, SE-22362 Lund, Sweden.
authors.forename: E
authors.initials: E
authors.lastname: Morfeldt
authors.forename: K
authors.initials: K
authors.lastname: Berggård
authors.forename: J
authors.initials: J
authors.lastname: Persson
authors.forename: T
authors.initials: T
authors.lastname: Drakenberg
authors.forename: E
authors.initials: E
authors.lastname: Johnsson
authors.forename: E
authors.initials: E
authors.lastname: Lindahl
authors.forename: S
authors.initials: S
authors.lastname: Linse
authors.forename: G
authors.initials: G
authors.lastname: Lindahl
mesh_terms: D000595:Amino Acid Sequence; D000940:Antigenic Variation; D000942:Antigens, Bacterial / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D001667:Binding, Competitive; D002352:Carrier Proteins / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D002942:Circular Dichroism; D003169:Complement Inactivator Proteins; D006023:Glycoproteins; D006801:Humans; D008969:Molecular Sequence Data; D010455:Peptides / Q000378:metabolism; D011951:Receptors, Complement / Q000378:metabolism*; D013297:Streptococcus pyogenes / Q000276:immunology* / Q000472:pathogenicity
chemical_list: D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D002352:Carrier Proteins; D003169:Complement Inactivator Proteins; D006023:Glycoproteins; D010455:Peptides; D011951:Receptors, Complement; C014689:streptococcal M protein