High-resolution and high-throughput protocols for measuring drug/human serum albumin interactions using BIACORE.
Characterizing how chemical compounds bind to human serum albumin (HSA) is essential in evaluating drug candidates. Using warfarin as a test system, we validate the application of BIACORE SPR biosensors to reliably determine binding constants for drug/HSA interactions. The binding responses for warfarin over HSA surfaces were extremely reproducible even though warfarin is small compared to the size of the immobilized protein. At high concentrations, warfarin bound at more than one site on HSA, which is consistent with its known binding properties. The affinity we determined for the high-affinity site (K(25 degrees C)(d) = 3.7 +/- 1.2 microM), as well as the dissociation rate constant (k(25 degrees C)(d) = 1.2 s(-1)), are also consistent with binding constants determined previously. These results validate the biosensor technology and illustrate how BIACORE can be used to study drug/HSA interactions in a high-resolution mode. Using a set of 10 test compounds, we present a protocol for determining equilibrium dissociation constants for HSA in a high-throughput mode. Our method involves working at low compound concentrations and fitting the equilibrium data for all compounds simultaneously. We show that the % bound values determined by SPR correlate with the values determined by solution-based methods. The ability to examine directly the binding of small molecules (130-800 Da), coupled with minimal sample requirements and automated instrumentation, makes BIACORE technology applicable for evaluating drug/HSA interactions.
Rich
R L
RL
Center for Biomolecular Interaction Analysis, University of Utah, 50 North Medical Drive, Room 4A417, Salt Lake City, Utah 84132, USA.
Day
Y S
YS
Morton
T A
TA
Myszka
D G
DG
D001665:Binding Sites; D004121:Dimethyl Sulfoxide / Q000737:chemistry; D006801:Humans; D015203:Reproducibility of Results; D012709:Serum Albumin / Q000378:metabolism*; D020349:Surface Plasmon Resonance / Q000379:methods*; D013696:Temperature; D013997:Time Factors; D014859:Warfarin / Q000378:metabolism*
D012709:Serum Albumin; D014859:Warfarin; D004121:Dimethyl Sulfoxide