title: Cellular adhesion in neoplastic and syngeneic normal cells under altered gravitational conditions.
abstract: The major objective of several experiments performed in space in the last 15 years was to establish whether single cells are sensitive to gravity. It was found in certain cells that reduced gravity leads to profound changes of a number  of  physiological  functions  like genetic expression, cell proliferation, signal transduction and cytoskeleton structure. In cell biology studies microgravity can be simulated on Earth in the clinostat. Nearly all data on experiments in the clinostat are related to cells cultured in suspension and, therefore, to adhesion-independent cells. In contrast, several biological phenomena as neoplastic transformation, cell differentiation, in-vitro cellular aging, contact inhibition and cellular adhesion require mainly cellular systems that are adhesion-dependent. The purpose of this work was: a) to study the behaviour of two rat cell strains (neoplastic SGS/4A and syngeneic fibroblasts FG) in order to test whether adhesion-dependent cells are suitable for clinorotation and b) to investigate cell-cell and cell-substratum adhesion in these cells kept under simulated low-g in the fast rotating clinostat and in hypergravity at l0g in the centrifuge.
authors.affiliation: Dipartimento di Scienze Fisiologiche, Biochimiche e Cellulari; Universita di Sassari.
authors.forename: P
authors.initials: P
authors.lastname: Pippia
authors.forename: M A
authors.initials: MA
authors.lastname: Meloni
authors.forename: G
authors.initials: G
authors.lastname: Cossu
authors.forename: M
authors.initials: M
authors.lastname: Cogoli-Greuter
authors.forename: A
authors.initials: A
authors.lastname: Cogoli
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002455:Cell Division; D002461:Cell Line, Transformed; D002498:Centrifugation; D005069:Evaluation Studies as Topic; D005347:Fibroblasts / Q000166:cytology*; D006112:Gravitation; D006801:Humans; D018471:Hypergravity; D019169:Jurkat Cells / Q000166:cytology; D008213:Lymphocyte Activation; D051381:Rats; D012399:Rotation; D013601:T-Lymphocytes / Q000166:cytology; D014407:Tumor Cells, Cultured / Q000166:cytology*; D018474:Weightlessness Simulation