Synthesis and analysis of RNA containing 6-trifluoromethylpurine ribonucleoside.
We report the synthesis of a 5'-DMT-2'-TBDMS-protected phosphoramidite of 6-trifluoromethylpurine ribonucleoside ((TFM)P) and its use in the site-specific incorporation of 6-trifluoromethylpurine into RNA. Properties of (TFM)P-substituted RNA suggest it will be valuable in the study of RNA structure and the binding of RNA-modifying enzymes, particularly the RNA-editing adenosine deaminases. Reaction: see text.
Véliz
E A
EA
Department of Chemistry, University of Utah, Salt Lake City, UT 84112, USA.
Stephens
O M
OM
Beal
P A
PA
D000577:Amides / Q000737:chemistry; D005461:Fluorine; D019906:Nuclear Magnetic Resonance, Biomolecular; D010756:Phosphoric Acids / Q000737:chemistry; D011684:Purine Nucleosides / Q000138:chemical synthesis* / Q000737:chemistry; D012313:RNA / Q000032:analysis / Q000138:chemical synthesis* / Q000737:chemistry; D015347:RNA Probes / Q000138:chemical synthesis / Q000737:chemistry; D020871:RNA Stability; D012263:Ribonucleosides / Q000138:chemical synthesis* / Q000737:chemistry
D000577:Amides; D010756:Phosphoric Acids; D011684:Purine Nucleosides; D015347:RNA Probes; D012263:Ribonucleosides; D005461:Fluorine; D012313:RNA; C011067:phosphoramidic acid