title: Sequence characterization of Ndelle virus genome segments 1, 5, 7, 8, and 10: evidence for reassignment to the genus Orthoreovirus, family Reoviridae.
abstract: The full-length nucleotide sequences of genome segments 1, 5, 7, 8 and 10 from Ndelle virus (NDEV) have been characterized. Comparison of the deduced protein amino acid sequences with those of other member viruses of the family Reoviridae demonstrates that NDEV was originally assigned incorrectly to the genus Orbivirus (aa identity values of <20%). In contrast, high levels of amino acid identity were found with members of the species Mammalian orthoreovirus (MRV); for example, amino acid identity in gamma3(Pol) is between 91 and 97%. These findings, together with previous antigenic analyses, provide evidence that NDEV should be reclassified as a new serotype (designated MRV-4) within the Mammalian orthoreovirus species.
authors.affiliation: Unité des Virus Emergents EA3292, Université de la Méditerranée and EFS Alpes-Méditerranée, Marseille, France.
authors.forename: H
authors.initials: H
authors.lastname: Attoui
authors.forename: P
authors.initials: P
authors.lastname: Biagini
authors.forename: J
authors.initials: J
authors.lastname: Stirling
authors.forename: P P
authors.initials: PP
authors.lastname: Mertens
authors.forename: J F
authors.initials: JF
authors.lastname: Cantaloube
authors.forename: A
authors.initials: A
authors.lastname: Meyer
authors.forename: P
authors.initials: P
authors.lastname: de Micco
authors.forename: X
authors.initials: X
authors.lastname: de Lamballerie
mesh_terms: D016679:Genome, Viral; D018165:Orthoreovirus / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D012330:RNA, Double-Stranded / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis*
chemical_list: D012330:RNA, Double-Stranded; D012367:RNA, Viral