Early events of polyoma infection:  adsorption, penetration and nuclear transport.
Polyoma virions have different attachment proteins which are responsible for hemagglutination of erythrocytes and attachment to cultured mouse kidney cells (MKC).  Virion binding studies demonstrated that MKC possess specific (productive infection) and nonspecific (nonproductive) receptors.  Empty polyoma capsids have hemagglutination activity and bind to non-specific MKC receptors, but they are not capable of competing for specific virion cell receptors or preventing productive infection.  Isoelectric focusing of the virion major capsid protein, VP1, separated this protein into six species (A through F).  These species had identical amino acid sequences, but differed in degree of modification (phosphorylation, acetylation, sulfation and hydroxylation).  Evidence based upon precipitation with specific antisera supports the view that VP1 species E is required for specific adsorption and that D and F are required for hemagglutination.  The virion attachment domain has been localized to an 18 kilodalton fragment of the C-terminal region of VP1.  Monopinocytotic vesicles containing 125I-labeled polyoma virions were isolated from infected MKC.  A crosslinker was used to bind the MKC cell receptor(s) covalently to VP1 attachment protein, and a new 120 kilodalton band was identified by SDS-PAGE.  An anti-idiotype antibody prepared against a neutralizing polyoma monoclonal antiody was used to identify a putative 50 kilodalton receptor protein from a detergent extract of MKC, as well as from MKC membrane preparation.
Consigli
R A
RA
Division of Biology, Kansas State University, Manhattan 66506, USA.
Haynes
J I
JI
Chang
D
D
Grenz
L
L
Richter
D
D
D000327:Adsorption; D000818:Animals; D001692:Biological Transport / Q000502:physiology; D002213:Capsid / Q000737:chemistry / Q000502:physiology*; D036022:Capsid Proteins; D002467:Cell Nucleus / Q000648:ultrastructure / Q000821:virology*; D002478:Cells, Cultured; D006168:Guinea Pigs; D006387:Hemagglutination, Viral; D007668:Kidney / Q000166:cytology; D051379:Mice; D030361:Papillomavirus Infections / Q000503:physiopathology*; D010873:Pinocytosis; D011120:Polyomavirus / Q000378:metabolism* / Q000502:physiology / Q000648:ultrastructure; D011991:Receptors, Virus / Q000502:physiology*; D014412:Tumor Virus Infections / Q000503:physiopathology*
D036022:Capsid Proteins; D011991:Receptors, Virus; C062481:VP1 protein, polyomavirus