title: Inhibition of human telomerase by L-enantiomers of natural 2'-deoxyribonucleoside 5'-triphosphates.
abstract: In order to clarify whether L-enantiomers of natural 2'-deoxyribonucleoside 5'-triphosphates (dNTPs) are recognized by human telomerase, a quantitative telomerase assay based on the 'stretch PCR' method was developed and used for kinetic analysis. Among the four L-dNTPs, L-dTTP and L-dGTP inhibited telomerase activity and the others showed slight or no inhibitory effect. Lineweaver-Burk plot analysis showed that the inhibition mode L-dGTP was competitive with dGTP.
authors.affiliation: Department of Biological Sciences, Biotechnology Research Center, Teikyo University of Science and Technology, Uenohara, Yamanashi 409-0193, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Yamaguchi
authors.forename: R
authors.initials: R
authors.lastname: Yamada
authors.forename: A
authors.initials: A
authors.lastname: Tomikawa
authors.forename: K
authors.initials: K
authors.lastname: Shudo
authors.forename: M
authors.initials: M
authors.lastname: Saito
authors.forename: F
authors.initials: F
authors.lastname: Ishikawa
authors.forename: M
authors.initials: M
authors.lastname: Saneyoshi
mesh_terms: D003848:Deoxyguanine Nucleotides / Q000378:metabolism / Q000494:pharmacology; D003854:Deoxyribonucleotides / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics; D016133:Polymerase Chain Reaction / Q000379:methods; D013237:Stereoisomerism; D019098:Telomerase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D013942:Thymine Nucleotides / Q000378:metabolism / Q000494:pharmacology
chemical_list: D003848:Deoxyguanine Nucleotides; D003854:Deoxyribonucleotides; D013942:Thymine Nucleotides; C029603:deoxyguanosine triphosphate; D019098:Telomerase; C024157:thymidine 5'-triphosphate