Oligonucleotides with isopolar phosphonate internucleotide linkage: a new perspective for antisense compounds?
Several types of isopolar modified oligothymidylates and oligoadenylates (15 mers) with the phosphonate -O-P-CH2-O- internucleotide linkage were prepared. The modified oligonucleotides were subjected to the study of their hybridization properties, resistance against nucleases, and the ability to elicit RNase H activity.
Rejman
D
D
Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences, Flemingovo nám. 2, 166 10 Prague 6, Czech Republic.
Snásel
J
J
Liboska
R
R
Tocík
Z
Z
Paces
O
O
Králíková
S
S
Rinnová
M
M
Kois
P
P
Rosenberg
I
I
D000249:Adenosine Monophosphate / Q000737:chemistry; D003432:Cross-Linking Reagents / Q000737:chemistry; D009693:Nucleic Acid Hybridization; D016376:Oligonucleotides, Antisense / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism; D063065:Organophosphonates / Q000737:chemistry*; D016914:Ribonuclease H / Q000378:metabolism; D013938:Thymidine Monophosphate / Q000737:chemistry
D003432:Cross-Linking Reagents; D016376:Oligonucleotides, Antisense; D063065:Organophosphonates; D013938:Thymidine Monophosphate; D000249:Adenosine Monophosphate; D016914:Ribonuclease H