title: Activation and localization of cartilage-derived morphogenetic protein-1 at the site of ossification of the ligamentum flavum.
abstract: Localization and expression of cartilage-derived morphogenetic protein (CDMP)-1 in tissues at the site of ossification of the ligamentum flavum (OLF) were examined by immunohistochemistry and in situ hybridization. The CDMP-1 protein and messenger ribonucleic acid (mRNA) were localized in spindle-shaped cells and chondrocytes in the OLF tissues. CDMP-1 was not detected in cells in non-ossified sites. These data indicate that CDMP-1 is locally activated and localized in spindle-shaped cells and chondrocytes at the site of OLE. Given the previously reported promoting action of CDMP-1 for chondrogenesis, the current results suggest that CDMP-1 may be involved in the progression of OLF, leading to the narrowing of spinal canal and thus causing severe clinical manifestations.
authors.affiliation: Department of Orthopaedic Surgery, Osaka University Medical School, Suita, Japan. nakase@ort.med.osaka-u.ac.jp
authors.forename: T
authors.initials: T
authors.lastname: Nakase
authors.forename: K
authors.initials: K
authors.lastname: Ariga
authors.forename: K
authors.initials: K
authors.lastname: Yonenobu
authors.forename: N
authors.initials: N
authors.lastname: Tsumaki
authors.forename: F P
authors.initials: FP
authors.lastname: Luyten
authors.forename: Y
authors.initials: Y
authors.lastname: Mukai
authors.forename: I
authors.initials: I
authors.lastname: Sato
authors.forename: H
authors.initials: H
authors.lastname: Yoshikawa
mesh_terms: D000368:Aged; D019485:Bone Morphogenetic Proteins; D019902:Chondrocytes / Q000378:metabolism; D055428:Growth Differentiation Factor 5; D006133:Growth Substances / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D017843:Ligamentum Flavum / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009999:Ossification, Heterotopic / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D016212:Transforming Growth Factor beta / Q000378:metabolism*
chemical_list: D019485:Bone Morphogenetic Proteins; D055428:Growth Differentiation Factor 5; D006133:Growth Substances; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta