title: A receptor-type guanylyl cyclase expression is regulated under circadian clock in peripheral tissues of the silk moth. Light-induced shifting of the expression rhythm and correlation with eclosion.
abstract: The mechanisms by which the circadian clock controls behavior through regulating gene expression in peripheral tissues are largely unknown. Here we demonstrate that the expression of a receptor-type guanylyl cyclase (BmGC-I) from the silk moth Bombyx mori is regulated in the flight muscles in a circadian fashion. BmGC-I mRNA was expressed from the end of the light period through the middle of the dark period. BmGC-I protein expression and cGMP levels were high around the initiation of eclosion events at the beginning of the photoperiod. The rhythm of the BmGC-I and cGMP levels free-ran in constant light and synchronized to the environmental photoperiodic cycle. The circadian regulation of BmGC-I expression was also observed in the legs but not in other tissues examined. BmGC-I therefore represents a circadian output gene that regulates eclosion behavior.
authors.affiliation: Laboratory of Insect Physiology, Graduate School of Agricultural Sciences, Kyoto University, 606-8502 Kyoto, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Tanoue
authors.forename: T
authors.initials: T
authors.lastname: Nishioka
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002940:Circadian Rhythm; D006152:Cyclic GMP / Q000378:metabolism; D006162:Guanylate Cyclase / Q000096:biosynthesis*; D007301:Insect Hormones / Q000096:biosynthesis*; D008024:Ligands; D008027:Light; D009036:Moths; D009132:Muscles / Q000378:metabolism; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors
chemical_list: D007301:Insect Hormones; D008024:Ligands; D012333:RNA, Messenger; C042645:eclosion hormone; D006162:Guanylate Cyclase; D006152:Cyclic GMP