title: Adhesion of Enterococcus faecalis 1131 grown under subinhibitory concentrations of ampicillin and vancomycin to a hydrophilic and a hydrophobic substratum.
abstract: The effect of two subinhibitory antibiotic concentrations of ampicillin and vancomycin during growth on the adhesion of Enterococcus faecalis 1131 to glass and silicone rubber was studied in a parallel plate flow chamber. Initial deposition rates and numbers of adhering bacteria after 4 h were higher on hydrophilic glass than on hydrophobic silicone rubber, regardless of growth conditions. The presence of 1/4 minimal inhibitory concentration (MIC) of ampicillin during growth reduced enterococcal adhesion to both substrata, but growth in the presence of 1/4 MIC vancomycin did not affect the adhesion of E. faecalis. Moreover, enterococcal adhesion increased after growth in the presence of 1/8 MIC vancomycin. The increased adhesion after growth in the presence of subinhibitory concentrations of vancomycin may have strong implications for patients living with implanted biomaterials, as they may suffer adverse effects from use of this antibiotic, especially since bacteria once adhered are less sensitive to antibiotics.
authors.affiliation: Department of Physics, Faculty of Sciences, Extremadura University, Avenida Elvas s/n 06071 Badajoz, Spain. amparogm@unex.es
authors.forename: A M
authors.initials: AM
authors.lastname: Gallardo-Moreno
authors.forename: H C
authors.initials: HC
authors.lastname: van der Mei
authors.forename: H J
authors.initials: HJ
authors.lastname: Busscher
authors.forename: M L
authors.initials: ML
authors.lastname: González-Martín
authors.forename: J M
authors.initials: JM
authors.lastname: Bruque
authors.forename: C
authors.initials: C
authors.lastname: Pérez-Giraldo
mesh_terms: D000667:Ampicillin / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001422:Bacterial Adhesion / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D013293:Enterococcus faecalis / Q000254:growth & development / Q000502:physiology*; D005898:Glass; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008826:Microbial Sensitivity Tests; D010406:Penicillins / Q000494:pharmacology*; D012408:Rubber; D012828:Silicones; D013997:Time Factors; D014640:Vancomycin / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D010406:Penicillins; D012828:Silicones; D014640:Vancomycin; D000667:Ampicillin; D012408:Rubber