title: G-quadruplex DNA binding by a series of carbocyanine dyes.
abstract: We have examined a number of carbocyanine dyes for their ability to bind intramolecular G-quadruplex DNA structures (G4'-DNA) using a Taq polymerase stop assay. Of the five dyes examined, only one, N,N'-diethylthiacarbocyanine iodide (DTC), was found to bind to G4'-DNA. DTC was also the only dye found to inhibit human telomerase at 50 microM concentration.
authors.affiliation: Division of Medicinal Chemistry, College of Pharmacy, University of Texas at Austin, 78712, USA. skerwin@mail.utexas.edu
authors.forename: S M
authors.initials: SM
authors.lastname: Kerwin
authors.forename: D
authors.initials: D
authors.lastname: Sun
authors.forename: J T
authors.initials: JT
authors.lastname: Kern
authors.forename: A
authors.initials: A
authors.lastname: Rangan
authors.forename: P W
authors.initials: PW
authors.lastname: Thomas
mesh_terms: D001665:Binding Sites; D002232:Carbocyanines / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*; D004396:Coloring Agents / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D004353:Drug Evaluation, Preclinical; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*; D006367:HeLa Cells; D006801:Humans; D009690:Nucleic Acid Conformation; D019914:Taq Polymerase / Q000037:antagonists & inhibitors; D019098:Telomerase / Q000037:antagonists & inhibitors
chemical_list: D002232:Carbocyanines; D004396:Coloring Agents; D004791:Enzyme Inhibitors; C448385:N,N'-diethylthiacarbocyanine iodide; D004247:DNA; D019914:Taq Polymerase; D019098:Telomerase