An effective AIDS vaccine based on live attenuated vesicular stomatitis virus recombinants.
We developed an AIDS vaccine based on attenuated VSV vectors expressing env and gag genes and tested it in rhesus monkeys. Boosting was accomplished using vectors with glycoproteins from different VSV serotypes. Animals were challenged with a pathogenic AIDS virus (SHIV89.6P). Control monkeys showed a severe loss of CD4+ T cells and high viral loads, and 7/8 progressed to AIDS with an average time of 148 days. All seven vaccinees were initially infected with SHIV89.6P but have remained healthy for up to 14 months after challenge with low or undetectable viral loads. Protection from AIDS was highly significant (p = 0.001). VSV vectors are promising candidates for human AIDS vaccine trials because they propagate to high titers and can be delivered without injection.
Rose
N F
NF
Department of Pathology, Yale University School of Medicine, New Haven, CT 06510, USA.
Marx
P A
PA
Luckay
A
A
Nixon
D F
DF
Moretto
W J
WJ
Donahoe
S M
SM
Montefiori
D
D
Roberts
A
A
Buonocore
L
L
Rose
J K
JK
D016915:AIDS Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology* / Q000517:prevention & control / Q000821:virology; D000818:Animals; D018791:CD4 Lymphocyte Count; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D015686:Gene Products, env / Q000235:genetics / Q000276:immunology; D015683:Gene Products, gag / Q000235:genetics / Q000276:immunology; D006678:HIV / Q000276:immunology / Q000502:physiology; D015483:HIV Antibodies / Q000096:biosynthesis; D006801:Humans; D007117:Immunization, Secondary; D008253:Macaca mulatta; D051379:Mice; D009500:Neutralization Tests; D010865:Pilot Projects; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology; D018449:SAIDS Vaccines / Q000235:genetics / Q000276:immunology; D016097:Simian Acquired Immunodeficiency Syndrome / Q000276:immunology / Q000517:prevention & control / Q000821:virology; D015302:Simian Immunodeficiency Virus / Q000276:immunology / Q000502:physiology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D013997:Time Factors; D014613:Vaccines, Attenuated / Q000235:genetics / Q000276:immunology; D014614:Vaccines, Synthetic / Q000276:immunology; D014721:Vesicular stomatitis Indiana virus / Q000235:genetics* / Q000276:immunology; D019562:Viral Load; D017201:Virus Shedding
D016915:AIDS Vaccines; D015686:Gene Products, env; D015683:Gene Products, gag; D015483:HIV Antibodies; D011993:Recombinant Fusion Proteins; D018449:SAIDS Vaccines; D014613:Vaccines, Attenuated; D014614:Vaccines, Synthetic