Regulation of the Arabidopsis transcriptome by oxidative stress.
Oxidative stress, resulting from an imbalance in the accumulation and removal of reactive oxygen species such as hydrogen peroxide (H(2)O(2)), is a challenge faced by all aerobic organisms. In plants, exposure to various abiotic and biotic stresses results in accumulation of H(2)O(2) and oxidative stress. Increasing evidence indicates that H(2)O(2) functions as a stress signal in plants, mediating adaptive responses to various stresses. To analyze cellular responses to H(2)O(2), we have undertaken a large-scale analysis of the Arabidopsis transcriptome during oxidative stress. Using cDNA microarray technology, we identified 175 non-redundant expressed sequence tags that are regulated by H(2)O(2). Of these, 113 are induced and 62 are repressed by H(2)O(2). A substantial proportion of these expressed sequence tags have predicted functions in cell rescue and defense processes. RNA-blot analyses of selected genes were used to verify the microarray data and extend them to demonstrate that other stresses such as wilting, UV irradiation, and elicitor challenge also induce the expression of many of these genes, both independently of, and, in some cases, via H(2)O(2).
Desikan
R
R
Centre for Research in Plant Science, University of the West of England, Bristol, Coldharbour Lane, Bristol BS16 1QY, United Kingdom.
A-H-Mackerness
S
S
Hancock
J T
JT
Neill
S J
SJ
D000222:Adaptation, Physiological; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D015152:Blotting, Northern; D020224:Expressed Sequence Tags; D018506:Gene Expression Regulation, Plant; D006861:Hydrogen Peroxide / Q000032:analysis / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D018384:Oxidative Stress; D020675:Peroxisomes / Q000378:metabolism; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism; D018749:RNA, Plant / Q000378:metabolism; D017385:Sequence Homology; D015398:Signal Transduction; D014158:Transcription, Genetic; D014466:Ultraviolet Rays; D014867:Water / Q000378:metabolism
D018749:RNA, Plant; D014867:Water; D006861:Hydrogen Peroxide