title: Increased levels of immune inhibitory CD34+ progenitor cells in the peripheral blood of patients with node positive head and neck squamous cell carcinomas and the ability of these CD34+ cells to differentiate into immune stimulatory dendritic cells.
abstract: OBJECTIVE
  This study determined whether mobilization of immune inhibitory CD34+ cells by head and neck squamous cell carcinomas (HNSCC) is most prominent in patients who are node positive and whether these CD34+ cells could differentiate into immune stimulatory dendritic cells.
  
  
  METHODS
  Peripheral blood from patients with head and neck cancer was used to measure the frequency of CD34+ cells and their capacity to differentiate into immune stimulatory dendritic cells.
  
  
  RESULTS
  This study demonstrated that increased CD34+ cell levels were most prominent in patients who were node positive and patients with recurrent disease. These CD34+ cells differentiated into dendritic cells that were able to present tetanus toxoid to autologous T-cells.
  
  
  CONCLUSIONS
  Immune suppressive CD34+ cells that are prominent in patients with HNSCC who are node positive are able to develop into immune stimulatory dendritic cells.
  
  
  CONCLUSIONS
  Differentiation of tumor-mobilized CD34+ cells into dendritic cells may be an immunotherapeutic approach to stimulate antitumor reactivity.
authors.affiliation: Department of Research Services, Hines VA Hospital, Hines, Illinois 60141, USA.
authors.forename: D M
authors.initials: DM
authors.lastname: Lathers
authors.forename: N
authors.initials: N
authors.lastname: Achille
authors.forename: K
authors.initials: K
authors.lastname: Kolesiak
authors.forename: K
authors.initials: K
authors.lastname: Hulett
authors.forename: A
authors.initials: A
authors.lastname: Sparano
authors.forename: G J
authors.initials: GJ
authors.lastname: Petruzzelli
authors.forename: M R
authors.initials: MR
authors.lastname: Young
mesh_terms: D017951:Antigen Presentation; D018952:Antigens, CD34 / Q000097:blood* / Q000502:physiology; D002294:Carcinoma, Squamous Cell / Q000097:blood / Q000276:immunology*; D002454:Cell Differentiation; D003713:Dendritic Cells / Q000276:immunology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000097:blood; D006258:Head and Neck Neoplasms / Q000097:blood / Q000276:immunology*; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D009364:Neoplasm Recurrence, Local / Q000276:immunology; D013601:T-Lymphocytes / Q000378:metabolism
chemical_list: D018952:Antigens, CD34; D007371:Interferon-gamma; D016178:Granulocyte-Macrophage Colony-Stimulating Factor