Fluorescence in situ hybridization analysis of peripheral blood cells in Pearson marrow-pancreas syndrome.
We used a dual-color fluorescence in situ hybridization technique to estimate deleted mitochondrial DNA at a single-cell level and determine any correlation with the disease progression in lymphocytes from patients with Pearson marrow-pancreas syndrome. The method demonstrated a shift in heteroplasmy, paralleling the hematologic improvement.
Yanagihara
I
I
Department of Environmental Medicine, Research Institute, Osaka Medical Center for Maternal and Child Health, Izumi City, Osaka, Japan.
Inui
K
K
Yanagihara
K
K
Park
Y D
YD
Tanaka
J
J
Ozono
K
K
Okada
S
S
Kurahashi
H
H
D000756:Anemia, Sideroblastic / Q000097:blood / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000097:blood*; D005260:Female; D006412:Hematopoietic Stem Cells / Q000473:pathology; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007223:Infant; D008214:Lymphocytes; D010182:Pancreatic Diseases / Q000097:blood / Q000235:genetics*; D013577:Syndrome
D004272:DNA, Mitochondrial