Simultaneous determination of cimetidine, famotidine, nizatidine, and ranitidine in tablets by capillary zone electrophoresis.
A simple capillary zone electrophoresis (CZE) method is described for the simultaneous determination of cimetidine (CIM), famotidine (FAM), nizatidine (NIZ), and ranitidine (RAN). The analysis of these drugs was performed in a 100 mM phosphate buffer, pH 3.5. Several parameters were studied, including wavelength for detection, concentration and pH of phosphate buffer, and separation voltage. The quantitative ranges were 100-1,000 microM for each analyte. The intra- and interday relative standard deviations (n = 5) were all less than 4%. The detection limits were found to be about 10 microM for CIM, 20 microM for RAN, 20 microM for NIZ, and 10 microM for FAM (S/N = 3, injection 1 s) at 214 nm. All recoveries were greater than 92%. Applications of the method to the assay of these drugs in tablets proved to be feasible.
Wu
S M
SM
School of pharmacy, College of Pharmacy, Kaohsiung Medical University, ROC, Taiwan. shmewu@kmu.edu.tw
Ho
Y H
YH
Wu
H L
HL
Chen
S H
SH
Ko
H S
HS
D000897:Anti-Ulcer Agents / Q000032:analysis*; D002021:Buffers; D002138:Calibration; D002927:Cimetidine / Q000032:analysis*; D015738:Famotidine / Q000032:analysis*; D006635:Histamine H2 Antagonists / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D015394:Molecular Structure; D016567:Nizatidine / Q000032:analysis*; D010710:Phosphates; D011899:Ranitidine / Q000032:analysis*; D013607:Tablets / Q000032:analysis; D013997:Time Factors
D000897:Anti-Ulcer Agents; D002021:Buffers; D006635:Histamine H2 Antagonists; D010710:Phosphates; D013607:Tablets; D015738:Famotidine; D002927:Cimetidine; D011899:Ranitidine; D016567:Nizatidine