title: Engineering mammalian cells for solid-state sensor applications.
abstract: A fundamental advance in the development and application of cell- and tissue-based biosensors would be the ability to achieve air-dry stabilization of mammalian (especially human) cells with subsequent recovery following rehydration. The would allow for the preparation of sensors with extended shelf lives, only requiring the addition of water for activation. By understanding and subsequently employing the tactics used by desiccation-tolerant extremophiles, it may be possible to design stabilized mammalian cell-based biosensors. The approaches required to realize this goal are discussed and illustrated with several examples.
authors.affiliation: Life Technologies-A Division of Invitrogen, Medical Center Drive, Rockville, MD 20850, USA.
authors.forename: F R
authors.initials: FR
authors.lastname: Bloom
authors.forename: P
authors.initials: P
authors.lastname: Price
authors.forename: G
authors.initials: G
authors.lastname: Lao
authors.forename: J L
authors.initials: JL
authors.lastname: Xia
authors.forename: J H
authors.initials: JH
authors.lastname: Crowe
authors.forename: J R
authors.initials: JR
authors.lastname: Battista
authors.forename: R F
authors.initials: RF
authors.lastname: Helm
authors.forename: S
authors.initials: S
authors.lastname: Slaughter
authors.forename: M
authors.initials: M
authors.lastname: Potts
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D001698:Biomedical Engineering / Q000379:methods*; D015374:Biosensing Techniques / Q000379:methods*; D002460:Cell Line; D003890:Desiccation; D005798:Genes, Bacterial; D006801:Humans; D011134:Polysaccharides; D013395:Sucrose / Q000378:metabolism; D014162:Transfection
chemical_list: D001426:Bacterial Proteins; D011134:Polysaccharides; C110344:SpsA protein, Streptococcus pneumoniae; D013395:Sucrose