title: Reduced release of DNA from streptococcus pneumoniae after treatment with rifampin in comparison to spontaneous growth and ceftriaxone treatment.
abstract: In order to study the release of DNA from Streptococcus pneumoniae in vitro during spontaneous growth and treatment with ceftriaxone or rifampin, a semiquantitative polymerase chain reaction was used. During spontaneous growth, high concentrations of bacterial DNA were released. Exposure to 10 microg/ml of ceftriaxone decreased the DNA release, in median, by 19 times (P=0.03 vs. spontaneous growth). Treatment with 10 microg/ml of rifampin led to a reduction of DNA release, in median, by a factor of 49 (P=0.03 vs. ceftriaxone; six experiments performed on different days).
authors.affiliation: Department of Neurology, University of Gottingen, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Gerber
authors.forename: H
authors.initials: H
authors.lastname: Eiffert
authors.forename: H
authors.initials: H
authors.lastname: Fleischer
authors.forename: A
authors.initials: A
authors.lastname: Wellmer
authors.forename: U
authors.initials: U
authors.lastname: Munzel
authors.forename: R
authors.initials: R
authors.lastname: Nau
mesh_terms: D001483:Base Sequence; D002443:Ceftriaxone / Q000494:pharmacology*; D004269:DNA, Bacterial / Q000032:analysis* / Q000187:drug effects*; D006801:Humans; D007124:Immunoenzyme Techniques; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011336:Probability; D012016:Reference Values; D012293:Rifampin / Q000494:pharmacology*; D012680:Sensitivity and Specificity; D013296:Streptococcus pneumoniae / Q000187:drug effects* / Q000254:growth & development*
chemical_list: D004269:DNA, Bacterial; D002443:Ceftriaxone; D012293:Rifampin