Studies on phytanoyl-CoA 2-hydroxylase and synthesis of phytanoyl-coenzyme A.
Phytanoyl-CoA 2-hydroxylase (PAHX), an iron(II) and 2-oxoglutarate-dependent oxygenase, catalyses an essential step in the mammalian metabolism of beta-methylated fatty acids. Phytanoyl-CoA was synthesised and used to develop in vitro assays for PAHX. The product of the reaction was confirmed as 2-hydroxyphytanoyl-CoA by NMR and mass spectrometric analyses. In accord with in vivo analyses, hydroxylation of both 3R and 3S epimers of the substrate was catalysed by PAHX. Both pro- and mature- forms of PAHX were fully active.
Kershaw
N J
NJ
The Oxford Centre for Molecular Sciences and The Dyson Perrins Laboratory, South Parks Road, OX1 3QY, Oxford, UK.
Mukherji
M
M
MacKinnon
C H
CH
Claridge
T D
TD
Odell
B
B
Wierzbicki
A S
AS
Lloyd
M D
MD
Schofield
C J
CJ
D003065:Coenzyme A / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D006899:Mixed Function Oxygenases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D010831:Phytanic Acid / Q000031:analogs & derivatives* / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization; D013237:Stereoisomerism
D011994:Recombinant Proteins; C095966:hydroxyphytanoyl-coenzyme A; C089917:phytanoyl-coenzyme A; D010831:Phytanic Acid; D006899:Mixed Function Oxygenases; C102623:PHYH protein, human; D003065:Coenzyme A