[Respiration of wheat roots during inhibition of phospholipase A2 by 4-bromphenacylbromide].
Dependence of oxygen consumption by wheat root cells on the activity of phospholipase A2 (PLA2) was studied. The treatment of excised roots with 4-bromophenacile bromide (BPB), a specific inhibitor of PLA2, caused a decrease in the content of free fatty acids (FFA) and in oxygen consumption of root cells. The latter was prevented by exogenous application of a mixture of FFA. A similar inhibitory effect was caused by BPB after the activation of root respiration by 2,4-dinitrophenol (DNP). These data suggest that FFA may be involved in the regulation of respiration through the formation of succinate. This is supported by the fact of reduction of DNP-induced stimulation of oxygen consumption by malonate, known to be an inhibitor of succinate dehydrogenase, and by stimulation of respiration by exogenous application of succinate.
Valitova
Iu N
IuN
Kazan Institute of Biochemistry and Biophysics, Kazan Centre RAS. valitova@sci.kcn.ru
Gordon
L Kh
LKh
Ogorodnikova
T I
TI
Lygin
A V
AV
Ruban
N F
NF
D000098:Acetophenones / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005230:Fatty Acids, Nonesterified / Q000378:metabolism; D008314:Malonates / Q000494:pharmacology; D010101:Oxygen Consumption; D010741:Phospholipases A / Q000037:antagonists & inhibitors*; D054467:Phospholipases A2; D018517:Plant Roots / Q000201:enzymology / Q000378:metabolism*; D013385:Succinate Dehydrogenase / Q000037:antagonists & inhibitors; D014908:Triticum / Q000201:enzymology / Q000378:metabolism*
D000098:Acetophenones; D004791:Enzyme Inhibitors; D005230:Fatty Acids, Nonesterified; D008314:Malonates; C030290:malonic acid; D013385:Succinate Dehydrogenase; D010741:Phospholipases A; D054467:Phospholipases A2; C006821:4-bromophenacyl bromide