Four color fret dye nucleotide terminators for DNA sequencing.
The synthesis of four color set of energy transfer-dye terminators (8a-8d) starting from p-iodo-beta-phenylalanine was accomplished and their utility in the sequencing reactions has been evaluated.
Rao
T S
TS
Amersham Pharmacia Biotech., 800 Centennial Avenue, Piscataway, New Jersey 08855, USA.
Nampalli
S
S
Lavrenov
K
K
Zhang
W
W
Xiao
H
H
Nelson
J
J
Kumar
S
S
D004735:Energy Transfer; D005456:Fluorescent Dyes / Q000138:chemical synthesis*; D010649:Phenylalanine / Q000031:analogs & derivatives* / Q000737:chemistry*; D017422:Sequence Analysis, DNA / Q000379:methods*; D013237:Stereoisomerism
D005456:Fluorescent Dyes; D010649:Phenylalanine; C049181:4-iodophenylalanine