A transgenic marker expressed on discrete populations during B-cell development.
We describe a transgenic mouse strain that selectively express a surface marker (huCD25) on transitional B cells, pre-B cells and a lineage unidentified bone marrow (BM) population. We show that a subpopulation of B cells in Peyer's patches, spleen, blood and BM expressed the transgenic huCD25 marker on the cell surface. In the spleen, the huCD25 expression was found on transitional B cells, that had not yet been recruited into the recirculating pool. In the BM a fraction of the B220low surface immunoglobulin (Ig) negative PB493+ pre-B cells were huCD25+. HuCD25 expression was also seen on practically all immature B cells while the mature recirculating B cells did not express huCD25. A huCD25+B220- cell population was also found in the BM that had not rearranged the Ig heavy chain locus and did not express the lineage markers CD3, T-cell receptor (TCR), CD19 and Mac-1. A low expression of CD4 on these cells may indicate that they represent a noncommitted, hematopoetic progenitor cell population.
Wrammert
J
J
Section for Immunology, Department of Cell and Molecular Biology, Lund University, Lund, Sweden. jens.wrammert@immuno.lu.se
Vingsbo Lundberg
C
C
Leanderson
T
T
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology* / Q000276:immunology; D015415:Biomarkers; D001854:Bone Marrow Cells / Q000166:cytology; D002454:Cell Differentiation; D002462:Cell Membrane / Q000276:immunology; D005260:Female; D015870:Gene Expression; D017930:Genes, Reporter; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000276:immunology; D017493:Leukocyte Common Antigens; D018655:Lymphocyte Count; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D016296:Mutagenesis; D015375:Receptors, Interleukin-2 / Q000096:biosynthesis* / Q000235:genetics; D014158:Transcription, Genetic; D019076:Transgenes
D015415:Biomarkers; D015375:Receptors, Interleukin-2; D017493:Leukocyte Common Antigens