title: Genetic changes in mammalian cells transformed by helium ions.
abstract: Midterm Syrian Hamster embryo (SHE) cells were employed to study high LET-radiation induced tumorigenesis.  Normal SHE cells (secondary passage) were irradiated with accelerated helium ions at an incident energy of 22 MeV/u (9-10 keV/micrometer).  Transformed clones were isolated after growth in soft agar of cells obtained from the foci of the initial monolayer plated postirradiation.  To study the progression process of malignant transformation, the transformed clones were followed by monolayer subculturing for prolonged periods of time.  Subsequently, neoplasia tests in nude mice were done.  In this work, however, we have focused on karyotypic changes in the banding patterns of the chromosomes during the early part of the progressive process of cell transformation for helium ion-induced transformed cells.
authors.affiliation: Dipartimento di Scienze Fisiche, Universita' di Napoli, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Durante
authors.forename: G
authors.initials: G
authors.lastname: Grossi
authors.forename: T C
authors.initials: TC
authors.lastname: Yang
authors.forename: R
authors.initials: R
authors.lastname: Roots
mesh_terms: D000818:Animals; D002455:Cell Division; D002471:Cell Transformation, Neoplastic / Q000528:radiation effects*; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D025063:Chromosome Disorders; D006224:Cricetinae; D004249:DNA Damage; D004622:Embryo, Mammalian / Q000166:cytology; D005260:Female; D006371:Helium / Q000009:adverse effects*; D007477:Ions; D018499:Linear Energy Transfer / Q000235:genetics*; D008647:Mesocricetus; D011839:Radiation, Ionizing; D012062:Relative Biological Effectiveness; D013997:Time Factors
chemical_list: D007477:Ions; D006371:Helium