title: Preparation of oligodeoxynucleotide 5'-triphosphates using solid support approach.
abstract: We report a synthetic procedure for conversion of oligonucleotides to their 5'-triphosphate derivatives with moderate yield. The oligonucleotides were synthesized on solid support using standard phosphoramidite protocols. The DMT protection group was removed and the 5'-OH was phosphitylated using 2-chloro-4H-1,3,2-benzodioxaphosphorin-4-one followed by reaction with tributyammonium pyrophosphate and iodine oxidation. After subsequent removal from support and complete deprotection, the products were isolated by anion-exchange HPLC chromatography. Structures of several 5'-triphosphate derivatives have been proven by phosphorus NMR, Mass-spectrometry and by HPLC comparison with authentic samples.
authors.affiliation: TriLink Biotechnologies, Inc., San Diego, California, USA.
authors.forename: A V
authors.initials: AV
authors.lastname: Lebedev
authors.forename: I I
authors.initials: II
authors.lastname: Koukhareva
authors.forename: T
authors.initials: T
authors.lastname: Beck
authors.forename: M M
authors.initials: MM
authors.lastname: Vaghefi
mesh_terms: D002851:Chromatography, High Pressure Liquid; D019906:Nuclear Magnetic Resonance, Biomolecular; D009841:Oligonucleotides / Q000138:chemical synthesis*; D010755:Organophosphates / Q000138:chemical synthesis*; D009943:Organophosphorus Compounds / Q000737:chemistry
chemical_list: D009841:Oligonucleotides; D010755:Organophosphates; D009943:Organophosphorus Compounds; C434331:phosphoramidite