Attenuated lapinized chinese strain of classical swine fever virus: complete nucleotide sequence and character of 3'-noncoding region.
The complete nucleotide sequence including precise 5'- and 3'-terminal non-coding regions (NCRs) of the attenuated lapinized Chinese strain (HCLV) of Classical Swine Fever Virus (CSFV) was determined from overlapping cDNA clones constructed by separated RT-PCR and rapid amplification of cDNA ends (RACE) methods. The genomic RNA of the HCLV strain consists of 12.310 nucleotides (nts) including 374 nts and 242nts in the 5'- and 3'-NCRs, respectively. It contains one large open reading frame (ORF) encoding a polyprotein of 3,898 amino acids with a calculated molecular weight of 437.6 kDa. There is one notable insertion of 12 continuous nts, CTTTTTTCTTTT in the 3'-NCR of HCLV genomic cDNA when compared with its parental virulent Shimen strain. Sequence alignment of partial 3'-NCR reveals two groups of CSFV vaccine strains carrying similar T-rich insertions at different positions in this region. Computer-predicted secondary structures suggest that T-rich insertion greatly change the structures and thus decrease the promoter functions of 3'-NCRs during the replications of these two groups of CSFV vaccine strains.
Wu
H X
HX
Department of Virology, College of Life Sciences, Wuhan University, PR China.
Wang
J F
JF
Zhang
C Y
CY
Fu
L Z
LZ
Pan
Z S
ZS
Wang
N
N
Zhang
P W
PW
Zhao
W G
WG
D020413:3' Untranslated Regions / Q000737:chemistry; D020121:5' Untranslated Regions / Q000737:chemistry; D000818:Animals; D001483:Base Sequence; D002681:China; D006692:Classical Swine Fever Virus / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D004279:DNA, Viral; D015723:Gene Library; D005818:Genetic Engineering; D016679:Genome, Viral; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009711:Nucleotides; D012367:RNA, Viral / Q000737:chemistry; D011817:Rabbits; D017423:Sequence Analysis, RNA; D012689:Sequence Homology, Nucleic Acid; D013552:Swine; D014774:Virulence
D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D004279:DNA, Viral; D009711:Nucleotides; D012367:RNA, Viral