title: Destruction of Escherichia coli O157:H7 and Salmonella enteritidis in cow manure composting.
abstract: Application of cow manure and composted manure in agricultural practice could potentially cause contamination of foodstuffs with pathogenic bacteria such as Salmonella Enteritidis and Escherichia coli O157:H7. In this study, rifampicin-resistant (RifR) E. coli O157:H7 and Salmonella Enteritidis at a level of 7 log CFU/g of raw compost feed were used to determine the effect of a bench-scale composting system on their survival. RifR E. coli O157:H7 was not detected after 72 h of composting at 45 degrees C, and RifR Salmonella Enteritidis was not detected after 48 h. The use of selective media for enrichment failed to recover in the composting samples held at 45 degrees C for 96 h. However, the pathogens showed no change in bacterial numbers when the composting system was held at room temperature. Thus, properly composted manure can be safely used in food crop production while minimizing the likelihood of microbial contamination.
authors.affiliation: Food Science and Human Nutrition Department, University of Florida, Gainesville 32611-0720, USA.
authors.forename: A J
authors.initials: AJ
authors.lastname: Lung
authors.forename: C M
authors.initials: CM
authors.lastname: Lin
authors.forename: J M
authors.initials: JM
authors.lastname: Kim
authors.forename: M R
authors.initials: MR
authors.lastname: Marshall
authors.forename: R
authors.initials: R
authors.lastname: Nordstedt
authors.forename: N P
authors.initials: NP
authors.lastname: Thompson
authors.forename: C I
authors.initials: CI
authors.lastname: Wei
mesh_terms: D000818:Animals; D002417:Cattle; D015169:Colony Count, Microbial; D004352:Drug Resistance, Microbial; D019453:Escherichia coli O157 / Q000254:growth & development* / Q000302:isolation & purification; D008372:Manure / Q000382:microbiology*; D012293:Rifampin / Q000494:pharmacology; D012477:Salmonella enteritidis / Q000254:growth & development* / Q000302:isolation & purification; D013696:Temperature; D013997:Time Factors
chemical_list: D008372:Manure; D012293:Rifampin