Interleukin-11 enhances cell survival of decidualized normal human endometrial stromal cells.
High expression of interleukin-11 (IL-11) and the IL-11 receptor alpha chain in developing decidual cells in mice has been reported, and mice lacking IL-11 receptor expression have been reported to show impaired implantation owing to defective decidualization. However, the direct effect of IL-11 on endometrial stromal cells has not been studied. In this study, we examined the direct effects of IL-11 on normal human endometrial stromal cells using an in vitro decidualization assay system. IL-11 enhanced cell viability and prolactin secretion of 8-Br-cAMP-induced decidualized cells but not of non-stimulated stromal cells. IL-11 dose-dependently enhanced the viability of stromal cells co-stimulated with 8-Br-cAMP and IL-11 without any significant effect on prolactin secretion from the cells. The extracellular matrix did not affect the effect of IL-11 on the viability of 8-Br-cAMP-stimulated stromal cells. These results indicate that IL-11 enhances the viability of 8-Br-cAMP-stimulated stromal cells and of decidualized stromal cells, and that the cell survival signals generated by IL-11 are independent of those generated by the extracellular matrix. IL-11 produced locally in decidual tissues may enhance the viability of decidualized stromal cells and possibly protect against cell damage during embryo implantation and trophoblastic invasion.
Tanaka
T
T
Department of Obstetrics and Gynecology, Wakayama Medical College, 811-1 Kimi-idera, Wakayama 641-8509, Japan.
Sakamoto
T
T
Miyama
M
M
Ogita
S
S
Umesaki
N
N
D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D003656:Decidua / Q000187:drug effects* / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D010064:Embryo Implantation; D004717:Endometrium / Q000166:cytology*; D005260:Female; D006801:Humans; D017370:Interleukin-11 / Q000008:administration & dosage / Q000494:pharmacology*; D011388:Prolactin / Q000378:metabolism; D017154:Stromal Cells / Q000187:drug effects* / Q000502:physiology
D017370:Interleukin-11; D015124:8-Bromo Cyclic Adenosine Monophosphate; D011388:Prolactin