Identification and sequence characterization of a novel HLA-A11 serological variant (HLA-A*1108).
In this report we describe the identification of a novel HLA-A*11 allele, HLA-A*1108, found in two individuals of a Spanish family. This new allele was detected during routine HLA typing by an atypical serological reactivity pattern and by inconclusive patterns obtained in DNA-based typing methods. The nucleotide sequence of exons 2 and 3 of HLA-A*1108 was identical to HLA-A*11011 except for two nucleotide substitutions at codons 152 (GCG-->GAG) and 156 (CAG-->CGG). These mutations change the non-charged amino acid alanine, at codon 152, to negative glutamic acid, and also non-charged glutamine, at codon 156, to positive arginine, which may explain its anomalous serological reactivity.
Palou
E
E
Servei d'Immunohematologia, Centre de Transfusió i Banc de Teixits, Barcelona, Spain. epalou@hg.vhebron.es
Santos
M
M
Gil
J
J
Campos
E
E
Nogués
N
N
Ribera
A
A
D000483:Alleles; D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D001120:Arginine; D001483:Base Sequence; D003062:Codon; D005091:Exons / Q000235:genetics; D014644:Genetic Variation / Q000276:immunology; D018698:Glutamic Acid; D015234:HLA-A Antigens / Q000235:genetics* / Q000276:immunology*; D059870:HLA-A11 Antigen; D006239:Haplotypes; D006801:Humans; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D010375:Pedigree; D020641:Polymorphism, Single Nucleotide; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid
D003062:Codon; D015234:HLA-A Antigens; D059870:HLA-A11 Antigen; D018698:Glutamic Acid; D001120:Arginine