Possible involvement of amino acid transporters on S-nitroso-cysteine-induced inhibition of arachidonic acid release in PC12 cells.
Previously, we proposed that S-nitroso-cysteine (SNC) was incorporated via the L-type-like amino acid transporters in rat brain slices. In PC12 cells (rat neuronal cell line), SNC inhibited [(3)H]arachidonic acid (AA) release induced by mastoparan (wasp venom peptide). We investigated the involvement of amino acid transporters on SNC-induced inhibition of [(3)H]AA release in PC12 cells. SNC inhibited mastoparan-stimulated [(3)H]AA release in a concentration-dependent manner in normal Na(+)- and low Na(+)-containing buffer. The inhibitory effect of 0.6 mM SNC in low Na(+) buffer decreased by 10 mM L-leucine, L-phenylalanine, L-methionine and L-cysteine. In contrast, L-alanine, L-threonine, L-valine or L-isoleucine showed very limited effects. Addition of L-leucine and L-phenylalanine, but not L-alanine or L-valine, also decreased the inhibitory effect of SNC on ionomycin/Na(3)VO(4)-stimulated [(3)H]AA release in normal Na(+) buffer. These findings suggest that SNC is incorporated via the amino acid transporters and inhibits AA release in PC12 cells.
Nemoto
T
T
Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, Chiba University, 263-8522, Chiba, Japan.
Horie
S
S
Okuma
Y
Y
Nomura
Y
Y
Murayama
T
T
D026905:Amino Acid Transport Systems / Q000378:metabolism*; D000596:Amino Acids / Q000493:pharmacokinetics; D000818:Animals; D016718:Arachidonic Acid / Q000493:pharmacokinetics*; D003545:Cysteine / Q000031:analogs & derivatives* / Q000493:pharmacokinetics*; D036341:Intercellular Signaling Peptides and Proteins; D015759:Ionomycin / Q000494:pharmacology; D007476:Ionophores / Q000494:pharmacology; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D020030:Nitric Oxide Donors / Q000493:pharmacokinetics*; D016716:PC12 Cells; D010455:Peptides; D051381:Rats; D026403:S-Nitrosothiols / Q000493:pharmacokinetics*; D014316:Tritium; D014638:Vanadates / Q000494:pharmacology; D014862:Wasp Venoms / Q000494:pharmacology
D026905:Amino Acid Transport Systems; D000596:Amino Acids; D036341:Intercellular Signaling Peptides and Proteins; D007476:Ionophores; D020030:Nitric Oxide Donors; D010455:Peptides; D026403:S-Nitrosothiols; D014862:Wasp Venoms; D014316:Tritium; D016718:Arachidonic Acid; D014638:Vanadates; D015759:Ionomycin; C037500:mastoparan; C021315:S-nitrosocysteine; D003545:Cysteine