title: Aptamers that bind to the antibiotic moenomycin A.
abstract: Nuclease-resistant moenomycin-binding aptamers with dissociation constants in the range of 300 to 400 nM have been selected. Competition experiments have demonstrated that these aptamers recognize a disaccharide analogue of moenomycin. The results offer the opportunity of setting up a selective and sensitive assay for identifying moenomycin biosynthetic precursors.
authors.affiliation: Universität Leipzig, Institut für Biochemie, Talstr. 33, D-04103, Leipzig, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Schürer
authors.forename: K
authors.initials: K
authors.lastname: Stembera
authors.forename: D
authors.initials: D
authors.lastname: Knoll
authors.forename: G
authors.initials: G
authors.lastname: Mayer
authors.forename: M
authors.initials: M
authors.lastname: Blind
authors.forename: H H
authors.initials: HH
authors.lastname: Förster
authors.forename: M
authors.initials: M
authors.lastname: Famulok
authors.forename: P
authors.initials: P
authors.lastname: Welzel
authors.forename: U
authors.initials: U
authors.lastname: Hahn
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology*; D001419:Bacteria / Q000254:growth & development; D001431:Bacteriological Techniques; D001455:Bambermycins / Q000737:chemistry / Q000494:pharmacology*; D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D020650:Combinatorial Chemistry Techniques; D004247:DNA / Q000096:biosynthesis; D004187:Disaccharides / Q000737:chemistry; D004352:Drug Resistance, Microbial; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009841:Oligonucleotides / Q000737:chemistry / Q000378:metabolism; D012313:RNA / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism*; D017423:Sequence Analysis, RNA
chemical_list: D000900:Anti-Bacterial Agents; D004187:Disaccharides; D009841:Oligonucleotides; D001455:Bambermycins; C437243:moenomycin A; D012313:RNA; D004247:DNA