Cyclohexene nucleic acids (CeNA) form stable duplexes with RNA and induce RNase H activity.
Cyclohexene nucleic acids (CeNA) were synthesized using classical phosporamidite chemistry. Incorporation of a cyclohexene nucleo-side in a DNA chain leads to an increase in stability of the DNA/RNA duplex. CeNA is stable against degradation in serum. A CeNA/RNA hybrid is able to activate E. Coli RNase H. resulting in cleavage of the RNA strand.
Wang
J
J
Laboratory for Medicinal Chemistry, Rega Institute, Katholieke Universiteit Leuven, Minderbroedersstraat 10, B-3000 Leuven, Belgium.
Verbeure
B
B
Luyten
I
I
Froeyen
M
M
Hendrix
C
C
Rosemeyer
H
H
Seela
F
F
Van Aerschot
A
A
Herdewijn
P
P
D002942:Circular Dichroism; D003510:Cyclohexanes / Q000737:chemistry* / Q000378:metabolism; D053138:Cyclohexenes; D004247:DNA / Q000737:chemistry; D004789:Enzyme Activation; D004926:Escherichia coli / Q000201:enzymology; D009696:Nucleic Acids / Q000737:chemistry* / Q000378:metabolism; D012313:RNA / Q000737:chemistry* / Q000378:metabolism; D016914:Ribonuclease H / Q000378:metabolism*; D013402:Sugar Alcohols / Q000737:chemistry
D003510:Cyclohexanes; D053138:Cyclohexenes; D009696:Nucleic Acids; D013402:Sugar Alcohols; C052568:cyclohexene; D012313:RNA; D004247:DNA; D016914:Ribonuclease H