Genomic analysis of synaptotagmin genes.
I used TBLASTn to probe DNA sequence databases with a consensus peptide sequence corresponding to the most highly conserved region of the rodent synaptotagmin (Syt) gene family, which is within the C2B domain. I found human homologues for all known rodent genes, and found six further human genomic loci which encode potential family members. I found eight potential family members in Caenorhabditis elegans, six in Drosophila melanogaster, and four in Arabidopsis thaliana. The C. elegans Syt1 homologue uniquely encodes two alternative C2B exons, one or the other of which is expressed at a time. Comparison of the genomic structures of the Syt genes makes clear the different phylogenies of the different subgroups. Knowledge of the genomic structures will aid the systematic investigation of alternative splicing in Syt genes.
Craxton
M
M
MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 2QH, UK. molly@mrc-lmb.cam.ac.uk
D000595:Amino Acid Sequence; D000818:Animals; D017360:Arabidopsis / Q000235:genetics; D015152:Blotting, Northern; D017173:Caenorhabditis elegans; D002135:Calcium-Binding Proteins; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D016208:Databases, Factual; D004331:Drosophila melanogaster / Q000235:genetics; D005260:Female; D015870:Gene Expression; D016678:Genome; D015894:Genome, Human; D006801:Humans; D008562:Membrane Glycoproteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D050857:Synaptotagmins; D014018:Tissue Distribution
D002135:Calcium-Binding Proteins; D018076:DNA, Complementary; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; C494288:SYT3 protein, human; D050857:Synaptotagmins