Automated selection of anti-protein aptamers.
The in vitro selection of nucleic acid binding species (aptamers) is frequently repetitive, time-consuming, and poorly adapted to high-throughput applications. We have adapted automated workstations to select anti-protein aptamers; as an example, we demonstrated the selection of anti-lysozyme aptamers that function as efficient inhibitors of cell lysis. The increases in throughput brought about by automation should potentiate the application of aptamer technology to the rapidly growing field of proteomics.
Cox
J C
JC
Institute for Cellular and Molecular Biology, University of Texas at Austin, 78712, USA.
Ellington
A D
AD
D000818:Animals; D001331:Automation / Q000379:methods; D001483:Base Sequence; D001665:Binding Sites; D019921:Biotinylation; D002645:Chickens; D004247:DNA / Q000032:analysis / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D008837:Micrococcus; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009113:Muramidase / Q000276:immunology*; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000032:analysis / Q000378:metabolism; D010957:Plasmids / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods; D011145:Polyvinyls; D011485:Protein Binding; D011489:Protein Denaturation; D011506:Proteins / Q000737:chemistry*; D012313:RNA / Q000032:analysis* / Q000378:metabolism; D017422:Sequence Analysis, DNA / Q000379:methods; D013997:Time Factors; D014158:Transcription, Genetic; D005465:Fluorocarbon Polymers
D004247:DNA; D012321:DNA-Directed RNA Polymerases; D009113:Muramidase; D009841:Oligonucleotides; D011145:Polyvinyls; D011506:Proteins; D012313:RNA; C024865:polyvinylidene fluoride; D005465:Fluorocarbon Polymers; C421864:hen egg lysozyme