The biological clock of Neurospora in a microgravity environment.
The circadian rhythm of conidiation in Neurospora crassa is thought to be an endogenously derived circadian oscillation; however, several investigators have suggested that circadian rhythms may, instead, be driven by some geophysical time cue(s).  An experiment was conducted on space shuttle flight STS-9 in order to test this hypothesis; during the first 7-8 cycles in space, there were several minor alterations observed in the conidiation rhythm, including an increase in the period of the oscillation, an increase in the variability of the growth rate and a diminished rhythm amplitude, which eventually damped out in 25% of the flight tubes.  On day seven of flight, the tubes were exposed to light while their growth fronts were marked.  Some aspect of the marking process reinstated a robust rhythm in all the tubes which continued throughout the remainder of the flight.  These results from the last 86 hours of flight demonstrated that the rhythm can persist in space.  Since the aberrant rhythmicity occurred prior to the marking procedure, but not after, it was hypothesized that the damping on STS-9 may have resulted from the hypergravity pulse of launch.  To test this hypothesis, we conducted investigations into the effects of altered gravitational forces on conidiation.  Exposure to hypergravity (via centrifugation), simulated microgravity (via the use of a clinostat) and altered orientations (via alterations in the vector of a 1 g force) were used to examine the effects of gravity upon the circadian rhythm of conidiation.
Ferraro
J S
JS
Department of Physiology, Southern Illinois University, Carbondale 62901, USA.
Fuller
C A
CA
Sulzman
F M
FM
D001683:Biological Clocks / Q000502:physiology* / Q000528:radiation effects; D002498:Centrifugation; D002940:Circadian Rhythm / Q000502:physiology* / Q000528:radiation effects; D006112:Gravitation; D018471:Hypergravity / Q000009:adverse effects; D008027:Light; D009492:Neurospora crassa / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D012399:Rotation; D013026:Space Flight; D013997:Time Factors; D014893:Weightlessness; D018474:Weightlessness Simulation