title: Peculiarities of the submicroscopic organization of Chlorella cells cultivated on a solid medium in microgravity.
abstract: The submicroscopic organization of Chlorella vulgaris cells (strain LARG-1) growing over 30 days on a solid agarized medium aboard the orbital station "Mir" was studied.  A number of differences in the ultrastructure of cells of the experimental population compared to the control has been revealed.  Thus, changes in the membrane system of plastids, in particular, appearance of numerous vesicles of different diameter and outgrowths of the plastids and their contact with the plasmalemma as well as a considerable decrease of reserve polysaccharide number in the plastids.  Moreover, an increase in the size of mitochondria, their cristae and lipid drops in cytoplasm, the formation of more complicated configuration folding of plasmalemma and appearance of small-granular material of mean electron density in the periplasmic space of Chlorella cells grown during space flight, are demonstrated.  Comparative cytological analysis has revealed general regularities of rearrangements of the submicroscopic organization in Chlorella cells cultivated on both solid and semiliquid agarized nutrient media.
authors.affiliation: N.G. Kholodny Institute of Botany of the Ukrainian Academy of Sciences, Kiev, USSR.
authors.forename: K M
authors.initials: KM
authors.lastname: Sytnik
authors.forename: A F
authors.initials: AF
authors.lastname: Popova
authors.forename: G S
authors.initials: GS
authors.lastname: Nechitailo
authors.forename: A L
authors.initials: AL
authors.lastname: Mashinsky
mesh_terms: D000362:Agar; D002462:Cell Membrane / Q000648:ultrastructure; D002708:Chlorella / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure*; D003470:Culture Media / Q000494:pharmacology; D007425:Intracellular Membranes / Q000648:ultrastructure; D008854:Microscopy, Electron; D008928:Mitochondria / Q000648:ultrastructure; D018087:Plastids / Q000648:ultrastructure; D013026:Space Flight; D014586:USSR; D014893:Weightlessness
chemical_list: D003470:Culture Media; D000362:Agar