Influence of phage population on the phage-mediated bioluminescent adenylate kinase (AK) assay for detection of bacteria.
OBJECTIVE
The effect of phage concentration on the activity of adenylate kinase (AK) released from the cells lysed during infection was investigated in order to optimize a bioluminescent phage-mediated method for bacterial enumeration.


RESULTS
The number of bacteria lysed by phages specific to Salmonella enteritidis and E. coli was determined using a bioluminescent method for the detection of AK released. In order to optimize the assay, the effect of phage concentration and time of infection on the amount of AK released was investigated. The release of AK was greatest at a multiplicity of infection (moi) of 10-100.


CONCLUSIONS
The amount of AK released from Salmonella enteritidis and E. coli G2-2 cells by specific phages, SJ2 and AT20, respectively, depended on the type of bacteria, the stage of growth, the nature of phage, moi and time.


CONCLUSIONS
An assay is described which allows detection of E. coli and Salmonella Enteritidis within 2 h at levels of 103 cfu ml-1.
Wu
Y
Y
Department of Food Science, University of Guelph, Guelph, Ontario N1G 2W1, Canada.
Brovko
L
L
Griffiths
M W
MW
D000263:Adenylate Kinase / Q000378:metabolism*; D001433:Bacteriolysis; D001435:Bacteriophages / Q000502:physiology*; D015169:Colony Count, Microbial; D004926:Escherichia coli / Q000201:enzymology* / Q000254:growth & development / Q000302:isolation & purification* / Q000821:virology; D008163:Luminescent Measurements; D012475:Salmonella / Q000201:enzymology* / Q000254:growth & development / Q000302:isolation & purification* / Q000821:virology; D012680:Sensitivity and Specificity; D013997:Time Factors
D000263:Adenylate Kinase