Validation of a new disposable counting chamber.
In a routine prospective study, 3 different counting chambers were compared for manual and computer-assisted evaluations. Swim-up samples were used so as to remove all debris and other cells that may contaminate the evaluation process when using a semen analyzer. The Makler concentration determinations (n = 20) were, on average. approximately 20 x 10(6) cells/mL higher compared to the corresponding 20 hemocytometer counts. The mean differences between the Leja chambers and the hemocytometer counts (n = 320) were only around 1 x 10(6) cells/mL, with coefficients of variation around 20%. The Leja chambers for both manual and the computer-assisted sperm concentration determinations provided consistent and accurate data on sperm concentration.
Coetzee
K
K
Department of Obstetrics and Gynecology, University of Stellenbosch and Tygerberg Hospital, South Africa.
Menkveld
R
R
D006801:Humans; D008297:Male; D011446:Prospective Studies; D013076:Sperm Count / Q000295:instrumentation*