DNA damage formation and p53 accumulation in mammalian cells exposed to the space environment.
To determine the effects of the space environment on gene instability from the point of view of human health for long-term stays in space, we have studied the formation of DNA strand breaks and the induction of gene expression in mammalian cells. We previously measured DNA damage in human cultured cells and the accumulation of a tumor suppressor gene product, p53, in muscle and skin of rats after space flight, and the relative importance of microgravity and space radiation in causing these effects remains to be clarified. Our results suggest that the p53 pathway may play a role in safeguarding genomic stability against the stressful space environment. We review here the present knowledge on cellular stress signaling and present our space experimental data. The importance of the stress response to the space environment is also discussed.
Ohnishi
T
T
Department of Biology, Nara Medical University, Japan. tohnishi@nmu-gw.naramed-u.ac.jp
Takahashi
A
A
Ohnishi
K
K
Matsumoto
H
H
D000818:Animals; D003359:Cosmic Radiation; D004249:DNA Damage; D004307:Dose-Response Relationship, Radiation; D015972:Gene Expression Regulation, Neoplastic; D006054:Goldfish; D050884:HSP72 Heat-Shock Proteins; D006367:HeLa Cells; D006360:Heat-Shock Proteins / Q000378:metabolism / Q000502:physiology; D006801:Humans; D018482:Muscle, Skeletal / Q000166:cytology / Q000378:metabolism / Q000502:physiology; D051381:Rats; D012062:Relative Biological Effectiveness; D015398:Signal Transduction; D013026:Space Flight; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000528:radiation effects; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism*; D014893:Weightlessness
D050884:HSP72 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D016159:Tumor Suppressor Protein p53