Immunohistochemical analysis of cyclins in canine normal testes and testicular tumors.
The expression of cyclins A, D1, D2 and E were examined immunohistochemically in 5 canine normal testes and 31 testicular tumors, including 14 seminomas, 11 Sertoli cell tumors and 6 Leydig cell tumors. In canine normal testes, cyclin A expression was detected in spermatogonia and primary spermatocytes. This suggests that A-type cyclins may play some role in canine spermatogenesis. Cyclin A expression was also observed in 13/14 (92.9%) seminomas and 2/11 (18.2%) Sertoli cell tumors, but no positive reaction was observed in Leydig cell tumors. Parallel examinations for cyclins D1, D2 and E gave negative results in canine normal testes and testicular tumors. High levels of cyclin A expression in canine seminomas indicate that the neoplastic germ cells may be arrested at the spermatogonia and primary spermatocyte stages of differentiation.
Murakami
Y
Y
Department of Veterinary Pathology, Faculty of Agriculture, Miyazaki University, Gakuen Kibana Dai, Japan.
Tateyama
S
S
Uchida
K
K
Yamaguchi
R
R
D000818:Animals; D016213:Cyclins / Q000096:biosynthesis*; D004283:Dog Diseases / Q000378:metabolism* / Q000473:pathology; D004285:Dogs; D007150:Immunohistochemistry; D007984:Leydig Cell Tumor / Q000378:metabolism / Q000473:pathology / Q000662:veterinary*; D008297:Male; D018239:Seminoma / Q000473:pathology / Q000662:veterinary*; D012707:Sertoli Cell Tumor / Q000378:metabolism / Q000473:pathology / Q000662:veterinary*; D013736:Testicular Neoplasms / Q000378:metabolism / Q000473:pathology / Q000662:veterinary*
D016213:Cyclins