Chemo-enzymatic synthesis of the Galili epitope Gal(alpha)(1-->3)Galbeta(1-->4)GlcNAc on a homogeneously soluble PEG polymer by a multi-enzyme system.
The alpha-Gal trisaccharide Gal(alpha)(1-->3)Galbeta(1-->4)GlcNAc 11 was synthesized on a homogeneously soluble polymeric support (polyethylene glycol, PEG) by use of a multi-enzyme system consisting of beta-1,4-galactosyltransferase (EC 2.4.1.38), alpha-1,3-galactosyltransferase (EC 2.4.1.151), sucrose synthase (EC 2.4.1.13) and UDP-glucose-4-epimerase (EC 5.1.3.2). In addition workup was simplified by use of dia-ultrafiltration. Thus the advantages of classic chemistry/enzymology and solid-phase synthesis could be united in one. Subsequent hydrogenolytic cleavage afforded the free alpha-Gal trisaccharide.
Brinkmann
N
N
Institute of Biotechnology, Forschungszentrum Juelich GmbH, 52425, Juelich, Germany.
Malissard
M
M
Ramuz
M
M
Römer
U
U
Schumacher
T
T
Berger
E G
EG
Elling
L
L
Wandrey
C
C
Liese
A
A
D001671:Biochemistry / Q000379:methods*; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D004798:Enzymes / Q000737:chemistry*; D000939:Epitopes / Q000737:chemistry*; D005700:Galactosyltransferases / Q000737:chemistry; D005964:Glucosyltransferases / Q000737:chemistry; D008969:Molecular Sequence Data; D009237:N-Acetyllactosamine Synthase / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D014312:Trisaccharides / Q000138:chemical synthesis*; D014534:UDPglucose 4-Epimerase / Q000737:chemistry
D004798:Enzymes; D000939:Epitopes; D014312:Trisaccharides; C076569:alpha-galactosyl epitope; D011092:Polyethylene Glycols; D005700:Galactosyltransferases; D005964:Glucosyltransferases; C022699:sucrose synthase; C047006:N-acetyllactosaminide alpha-1,3-galactosyltransferase; D009237:N-Acetyllactosamine Synthase; D014534:UDPglucose 4-Epimerase