title: Effect of simulated weightlessness on TNF-alpha production and the response of bone marrow cells to GM-CSF in rats.
abstract: The purpose of this experiment was to investigate the effect of 15 and 30 d simulated weightlessness (SWL) on tumor necrosis factor-alpha(TNF-alpha) production secreted by macrophage stimulated with lipopolysaccharide(LPS) and the ability of bone marrow cells to respond to granulocyte/monocyte colony-stimulating factor(GM-CSF) in tail-suspension rats. The TNF-alpha production was assessed by radioimmunoassay. The ability of bone marrow cells to respond to GM-CSF was measured by 3H-TdR incorporation method. The results showed that after 15 d SWL, TNF-alpha production showed a tendency to decrease; and that after 30 d SWL, the decrease became significant (P < 0.05). The results also demonstrated that the ability of bone marrow cells to respond to GM-CSF decreased significantly after 15 d SWL (P < 0.01); and there was no significant change after 30 d SWL. It suggested that SWL could depress immune function, and that reduction of TNF-alpha production might be related to bone marrow cell proliferation function.
authors.affiliation: Institute of Space Medico-Engineering, Beijing, China.
authors.forename: T
authors.initials: T
authors.lastname: Wang
authors.forename: G
authors.initials: G
authors.lastname: Yang
authors.forename: P
authors.initials: P
authors.lastname: Hu
authors.forename: X
authors.initials: X
authors.lastname: Wen
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000276:immunology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000276:immunology*; D019417:Hindlimb Suspension / Q000009:adverse effects*; D008264:Macrophages / Q000276:immunology* / Q000378:metabolism; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013026:Space Flight; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000378:metabolism*; D014893:Weightlessness; D018474:Weightlessness Simulation / Q000009:adverse effects*
chemical_list: D014409:Tumor Necrosis Factor-alpha; D016178:Granulocyte-Macrophage Colony-Stimulating Factor