[Immunogenetic study of the HLA system in families of patients with ankylosing spondylitis].
In this study the immunogenetic relationships among 141 unrelated HLA-B27+ patients with ankylosing spondylitis (AS) and 792 members of their families were studied. Two control groups, with at least one B27+ parent were used (families undergoing transplantation program and triplet families undergoing paternity testing). All subjects were typed for HLA-A and -B antigens by microlyphocytotoxity test (MLCT) on local typing trays. The frequency of HLA-A and -B alleles was equal in the all tested groups. The segregation of all tested genes was regular regarding to the total number of positive and negative siblings, while regarding to the sex of sibs was irregular for HLA-B27 and -B5 gene. The statistical significance (p < 0.05) was found when ratio between B27+ and B27- sons in AS group was compared with the same ration in control families. In AS group was detected statistical significant (p < 0.01) high number of B5+ than B5- daughters and statistical significant (p < 0.05) less number of B5+ sons. HLA-B21 was shown to be decreased among B27+ AS patients. A synergistic effect between additional HLA-B alleles and B27 was not observed. The distribution of B27 haplotypes in AS and control families was similar except for haplotype HLA-A10, B27 which was significant (p < 0.001) less present in AS families.
Grubić
Z
Z
Zavod za tipizaciju tkiva, Klinika za urologiju, KBC Zagreb, 10000 Zagreb.
Kerhin-Brkljacić
V
V
Cecuk-Jelicić
E
E
Kastelan
A
A
D003601:Cytotoxicity Tests, Immunologic; D005260:Female; D006680:HLA Antigens / Q000235:genetics*; D015234:HLA-A Antigens / Q000235:genetics; D015235:HLA-B Antigens / Q000235:genetics; D015796:HLA-B27 Antigen / Q000235:genetics; D006801:Humans; D008297:Male; D013167:Spondylitis, Ankylosing / Q000235:genetics* / Q000276:immunology
D006680:HLA Antigens; D015234:HLA-A Antigens; D015235:HLA-B Antigens; D015796:HLA-B27 Antigen