Characterization of catalase and superoxide dismutase in Staphylococcus carnosus 833 strain.
OBJECTIVE
Staphylococcus carnosus, used as starter culture in fermented meat products, decreases the level of volatiles arising from lipid oxidation. To analyse its antioxidant capacities, catalase and superoxide dismutase (SOD) were characterized.


RESULTS
Catalase and SOD activities were measured with spectrophotometric methods and visualized on non-denaturing polyacrylamide gels. The corresponding sod gene was identified by PCR. Southern hybridizations and enzymatic analyses showed that there was a single catalase and a single SOD in Staph. carnosus 833 strain. The gene encoding the Staph. carnosus SOD was found to encode a protein closely related to SOD requiring manganese. Catalase and SOD levels increased in mid-log phase. Only catalase was induced by oxygen, nitrate or nitrite while glucose induced neither enzyme. Metal ion limitation increased catalase and decreased SOD activities.


CONCLUSIONS
Staph. carnosus synthesizes both enzymes in conditions encountered in sausage manufacturing. These results could explain the antioxidant properties of Staph. carnosus starter culture.


CONCLUSIONS
The knowledge of the antioxidant properties of Staphylococci will allow a more rational use of these starters in meat fermented products.
Barrière
C
C
SRV Microbiologie, INRA, Centre de Clermont-Theix, F-63122 Saint-Genès Champanelle, France.
Leroy-Sétrin
S
S
Talon
R
R
D000975:Antioxidants / Q000378:metabolism; D015139:Blotting, Southern; D002374:Catalase / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D005285:Fermentation; D007700:Kinetics; D008460:Meat / Q000382:microbiology; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D009195:Peroxidase / Q000235:genetics / Q000378:metabolism; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013210:Staphylococcus / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism*
D000975:Antioxidants; D002374:Catalase; D009195:Peroxidase; D013482:Superoxide Dismutase