PNA-related oligonucleotide mimics and their evaluation for nucleic acid hybridization studies and analysis.
DNA mimics containing phosphonate analogues of PNAs (pPNAs), particularly PNA-pPNA hybrids as well as hetero-oligomers consisted of pPNA units and PNA-like molecules on the base of trans-4-hydroxy-L-proline (HypNA) have been synthesized. The evaluation of their effectiveness in assays based on the hybridization technique in the comparison with natural oligonucleotides and classical PNAs has shown a high potential of these mimics as sensor molecules for nucleic acid based diagnostics and as molecular probes for mRNA isolation.
Efimov
V
V
Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry, 117871 Moscow, Russia.
Choob
M
M
Buryakova
A
A
Phelan
D
D
Chakhmakhcheva
O
O
D015374:Biosensing Techniques; D004247:DNA / Q000737:chemistry; D018716:Molecular Mimicry; D009693:Nucleic Acid Hybridization / Q000379:methods*; D009841:Oligonucleotides / Q000737:chemistry*; D020135:Peptide Nucleic Acids / Q000737:chemistry*; D012333:RNA, Messenger / Q000302:isolation & purification
D009841:Oligonucleotides; D020135:Peptide Nucleic Acids; D012333:RNA, Messenger; D004247:DNA