title: Amphibian egg cytoplasm response to altered g-forces and gravity orientation.
abstract: Elucidation of dorsal/ventral polarity and primary embryonic axis development in amphibian embryos requires an understanding of cytoplasmic rearrangements in fertile eggs at the biophysical, physiological, and biochemical levels.  Evidence is presented that amphibian egg cytoplasmic components are compartmentalized.  The effects of altered orientation to the gravitational vector (i.e., egg inversion) and alterations in gravity force ranging from hypergravity (centrifugation) to simulated microgravity (i.e., horizontal clinostat rotation) on cytoplasmic compartment rearrangements are reviewed.  The behavior of yolk compartments as well as a newly defined (with monoclonal antibody) non-yolk cytoplasmic compartment, in inverted eggs and in eggs rotated on horizontal clinostats at their buoyant density, is discussed.
authors.affiliation: Medical Sciences Program, School of Medicine, Indiana University, Bloomington 47405.
authors.forename: A W
authors.initials: AW
authors.lastname: Neff
authors.forename: R C
authors.initials: RC
authors.lastname: Smith
authors.forename: G M
authors.initials: GM
authors.lastname: Malacinski
mesh_terms: D000818:Animals; D002451:Cell Compartmentation / Q000502:physiology*; D016764:Cell Polarity / Q000502:physiology*; D003593:Cytoplasm / Q000502:physiology* / Q000648:ultrastructure; D004625:Embryo, Nonmammalian; D006112:Gravitation; D010063:Ovum / Q000502:physiology / Q000648:ultrastructure; D012399:Rotation; D018474:Weightlessness Simulation; D014982:Xenopus laevis / Q000196:embryology*