In vitro effects of isoprinosine and a dipeptide methyl ester on Echinococcus multilocularis protoscoleces.
A protoscoleces/vesicles in vitro maintenance test with assessment of viability by eosin exclusion was used to evaluate the quantitative and qualitative activities of isoprinosine, its active component inosine and the dipeptide methylester L-Phe-Phe-OMe on isolated protoscoleces of Echinococcus multilocularis for 24 and 48 h. Isoprinosine and inosine showed dose- and time-dependent activity, the latter displaying a more rapid effect than the former. A high activity was shown with L-Phe-Phe-OMe, when compared to praziquantel. Ultrastructural alterations were much more striking with L-Phe-Phe-OMe, with an effect similar to that of praziquantel, whereas the chemotherapeutic activity of inosine and isoprinosine appeared to be directed against a metabolic target, with a lethal effect not immediately visible at the ultrastructural level. Thus, the previously reported in vivo activities of these drugs result largely from a direct effect on the parasite.
Lawton
P
P
Département de Parasitologie et Mycologie Médicale, Faculté de Pharmacie, Lyon, France. lawton@univ-lyon1.fr.
Walchshofer
N
N
Sarciron
M E
ME
D000818:Animals; D000871:Anthelmintics / Q000494:pharmacology*; D004151:Dipeptides / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004446:Echinococcus / Q000187:drug effects* / Q000648:ultrastructure; D007288:Inosine / Q000494:pharmacology; D007542:Inosine Pranobex / Q000494:pharmacology*; D008858:Microscopy, Phase-Contrast; D010274:Parasitology / Q000379:methods; D011223:Praziquantel / Q000494:pharmacology
D000871:Anthelmintics; D004151:Dipeptides; C063009:phenylalanylphenylalanine methyl ester; D007288:Inosine; D011223:Praziquantel; D007542:Inosine Pranobex