A comparison of RNA with DNA in template-directed synthesis.
Nonenzymatic template-directed copying of RNA sequences rich in cytidylic acid using nucleoside 5'-(2-methylimidazol-1-yl phosphates) as substrates is substantially more efficient than the copying of corresponding DNA sequences. However, many sequences cannot be copied, and the prospect of replication in this system is remote, even for RNA. Surprisingly, wobble-pairing leads to much more efficient incorporation of G opposite U on RNA templates than of G opposite T on DNA templates.
Zielinski
M
M
The Salk Institute for Biological Studies, San Diego, CA 92186, USA.
Kozlov
I A
IA
Orgel
L E
LE
D020029:Base Pairing; D001483:Base Sequence; D003568:Cytidine Monophosphate / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D017931:DNA Primers; D003854:Deoxyribonucleotides / Q000737:chemistry; D019020:Directed Molecular Evolution; D019143:Evolution, Molecular; D018559:Exobiology; D009841:Oligonucleotides / Q000138:chemical synthesis; D012313:RNA / Q000737:chemistry*; D012265:Ribonucleotides / Q000737:chemistry; D013698:Templates, Genetic
D017931:DNA Primers; D003854:Deoxyribonucleotides; D009841:Oligonucleotides; D012265:Ribonucleotides; D012313:RNA; D004247:DNA; D003568:Cytidine Monophosphate