title: Calcium phosphate crystal-associated proteins: alpha-2-HS-glycoprotein, prothrombin fragment 1 and osteopontin.
abstract: To study the inhibitory effects of calcium phosphate-associated proteins on calcium oxalate crystallization and urinary concentrations of proteins in people who form stones and healthy controls. From 60 L of urine from healthy men, calcium phosphate-associated proteins (alpha-2-HS-glycoprotein, prothrombin fragment 1 and osteopontin) were obtained. The effects of the proteins on calcium oxalate (CaOx) crystallization were studied with a mixed suspension mixed product removal system. To examine urinary concentrations of the proteins, urine samples were collected from 17 healthy subjects and 15 stone formers and analyzed using anion-exchange chromatography and an enzyme immunoassay. Prothrombin fragment 1 (PTF1) and osteopontin (OPN) had strong inhibitory effects on CaOx crystallization, while alpha-2-HS-glycoprotein had a mild inhibitory effect. Urinary concentrations of PTF1 and OPN were lower in stone formers than in healthy controls. Low urinary concentrations of PTF1 and OPN might be one of the reasons for stone formation.
authors.affiliation: Department of Urology, Ehime University, School of Medicine, Ehime, Japan. nishio@m.ehime-u.ac.jp
authors.forename: S
authors.initials: S
authors.lastname: Nishio
authors.forename: M
authors.initials: M
authors.lastname: Hatanaka
authors.forename: H
authors.initials: H
authors.lastname: Takeda
authors.forename: K
authors.initials: K
authors.lastname: Aoki
authors.forename: T
authors.initials: T
authors.lastname: Iseda
authors.forename: H
authors.initials: H
authors.lastname: Iwata
authors.forename: M
authors.initials: M
authors.lastname: Yokoyama
mesh_terms: D000328:Adult; D001798:Blood Proteins / Q000652:urine*; D002129:Calcium Oxalate / Q000737:chemistry*; D003460:Crystallization; D005260:Female; D006801:Humans; D008297:Male; D053495:Osteopontin; D010446:Peptide Fragments / Q000652:urine*; D010750:Phosphoproteins / Q000652:urine*; D011498:Protein Precursors / Q000652:urine*; D011516:Prothrombin / Q000652:urine*; D012795:Sialoglycoproteins / Q000652:urine*; D014545:Urinary Calculi / Q000652:urine*; D060749:alpha-2-HS-Glycoprotein
chemical_list: C556354:AHSG protein, human; D001798:Blood Proteins; D010446:Peptide Fragments; D010750:Phosphoproteins; D011498:Protein Precursors; C506895:SPP1 protein, human; D012795:Sialoglycoproteins; D060749:alpha-2-HS-Glycoprotein; D053495:Osteopontin; D002129:Calcium Oxalate; C029733:prothrombin fragment 1; D011516:Prothrombin