Inhibition of in vitro pre-mRNA splicing in S. cerevisiae by branched oligonucleotides.
A series of V- and Y-shaped nucleic acids, related to the splicing intermediates derived from S. cerevisiae actin pre-mRNA, were prepared. The effects of such branched nucleic acids (bNAs) on the efficiency of in vitro pre-mRNA splicing in yeast were studied. The exogenous bNAs each effect the efficiency of splicing, yet to different degrees, depending on the sugar composition and topology of the molecules. Y-shaped RNAs inhibited the formation of mRNA (i.e. RNA splicing) to the greatest extent.
Carriero
S
S
Department of Chemistry, McGill University, Montreal, QC, Canada, H3A 2K6.
Braich
R S
RS
Hudson
R H
RH
Anglin
D
D
Friesen
J D
JD
Damha
M J
MJ
D009841:Oligonucleotides / Q000235:genetics / Q000494:pharmacology*; D012322:RNA Precursors / Q000037:antagonists & inhibitors* / Q000378:metabolism; D012326:RNA Splicing / Q000187:drug effects*; D012333:RNA, Messenger / Q000037:antagonists & inhibitors* / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism
D009841:Oligonucleotides; D012322:RNA Precursors; D012333:RNA, Messenger