title: RNA association defines a functionally conserved domain in the nuclear pore protein Nup153.
abstract: Traffic between the nucleus and cytoplasm takes place through a macromolecular structure termed the nuclear pore complex. To understand how the vital process of nucleocytoplasmic transport occurs, the contribution of individual pore proteins must be elucidated. One such protein, the nucleoporin Nup153, is localized to the nuclear basket of the pore complex and has been shown to be a central component of the nuclear transport machinery. Perturbation of Nup153 function was demonstrated previously to block the export of several classes of RNA cargo. Moreover, these studies also showed that Nup153 can stably associate with RNA in vitro. In this study, we have mapped a domain within Nup153, encompassing amino acids 250-400 in human Nup153, that is responsible for RNA association. After cloning this region of Xenopus Nup153, we performed a cross-species analysis. Despite variation in sequence conservation between Drosophila, Xenopus, and human, this domain of Nup153 displayed robust RNA binding activity in each case, indicating that this property is a hallmark feature of Nup153 and pointing toward a subset of amino acid residues that are key to conferring this ability. We have further determined that a recombinant fragment of Nup153 can bind directly to RNA and that this fragment can interact with endogenous RNA targets. Our findings identify a functionally conserved domain in Nup153 and suggest a role for RNA binding in Nup153 function at the nuclear pore.
authors.affiliation: Department of Oncological Sciences, Huntsman Cancer Institute, University of Utah, Salt Lake City, Utah 84112, USA.
authors.forename: C
authors.initials: C
authors.lastname: Dimaano
authors.forename: J R
authors.initials: JR
authors.lastname: Ball
authors.forename: A J
authors.initials: AJ
authors.lastname: Prunuske
authors.forename: K S
authors.initials: KS
authors.lastname: Ullman
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry; D000818:Animals; D015153:Blotting, Western; D002467:Cell Nucleus / Q000378:metabolism*; D003001:Cloning, Molecular; D017124:Conserved Sequence; D004330:Drosophila; D019143:Evolution, Molecular; D006801:Humans; D008969:Molecular Sequence Data; D028861:Nuclear Pore Complex Proteins / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism*; D009865:Oocytes / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D012313:RNA / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D014981:Xenopus; D029867:Xenopus Proteins / Q000276:immunology*
chemical_list: D000596:Amino Acids; C079377:NUP153 protein, human; D028861:Nuclear Pore Complex Proteins; C519169:Nup153 protein, Xenopus; D011994:Recombinant Proteins; D029867:Xenopus Proteins; D012313:RNA