Two new point mutations at A2062 associated with resistance to 16-membered macrolide antibiotics in mutant strains of Mycoplasma hominis.
We describe two mutants of Mycoplasma hominis PG-21 which show resistance to 16-membered macrolides but susceptibility to lincosamides, obtained by in vitro exposure to increasing doses of josamycin. The 23S rRNA gene showed that each had a mutation (A2062G and A2062T) corresponding to nucleotide 2062 in Escherichia coli, which was associated with the acquired phenotype.
Furneri
P M
PM
Dipartimento di Scienze Microbiologiche e Scienze Ginecologiche, Università degli Studi, Via Androne 81, 95124 Catania, Italy. furneri@mbox.unict.it
Rappazzo
G
G
Musumarra
M P
MP
Di Pietro
P
P
Catania
L S
LS
Roccasalva
L S
LS
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001483:Base Sequence; D004269:DNA, Bacterial / Q000032:analysis; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D018942:Macrolides; D008969:Molecular Sequence Data; D019535:Mycoplasma hominis / Q000187:drug effects / Q000235:genetics*; D010641:Phenotype; D017354:Point Mutation; D012338:RNA, Ribosomal, 23S / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D018942:Macrolides; D012338:RNA, Ribosomal, 23S