title: Microgravity effects on neural retina regeneration in the newt.
abstract: Data on forelimb and eye lens regeneration in urodeles under spaceflight conditions (SFC) have been obtained in our previous studies. Today, evidence is available that SFC stimulate regeneration in experimental animals rather than inhibit it. The results of control on-ground experiments with simulated microgravity suggest that the stimulatory effect of SFC is due largely to weightlessness. An original experimental model is proposed, which is convenient for comprehensively analyzing neural regeneration under SFC. The initial results described here concern regeneration of neural retina in Pleurodeles waltl newts exposed to microgravity simulated in radial clinostat. After clinorotation for seven days (until postoperation day 16), a positive effect of altered gravity on structural restoration of detached neural retina was confirmed by a number of criteria. Specifically, an increased number of Mullerian glial cells, an increased relative volume of the plexiform layers, reduced cell death, advanced redifferentiation of retinal pigment epithelium, and extended areas of neural retina reattachment were detected in experimental newts. Moreover, cell proliferation in the inner nuclear layer of neural retina increased as compared with control.  Thus, low gravity appears to intensify natural cytological and molecular mechanisms of neural retina regeneration in lower vertebrates.
authors.affiliation: Institute of Developmental Biology, Russian Academy of Sciences, Moscow, Russia.
authors.forename: E N
authors.initials: EN
authors.lastname: Grigoryan
authors.forename: H J
authors.initials: HJ
authors.lastname: Anton
authors.forename: V I
authors.initials: VI
authors.lastname: Mitashov
mesh_terms: D000818:Animals; D016923:Cell Death; D002455:Cell Division; D006112:Gravitation; D008264:Macrophages / Q000502:physiology; D009457:Neuroglia / Q000502:physiology; D010857:Pigment Epithelium of Eye / Q000166:cytology / Q000502:physiology*; D010999:Pleurodeles / Q000502:physiology*; D012038:Regeneration / Q000502:physiology*; D012160:Retina / Q000166:cytology / Q000502:physiology* / Q000601:surgery; D012163:Retinal Detachment; D012399:Rotation; D018474:Weightlessness Simulation