Mannitol inhibits growth of intact cucumber but not pea seedlings by mechanically collapsing the root pressure.
The positive xylem pressure (Px) in cucumber hypocotyls is a direct extension of root pressure and therefore depends on the root environment. Solutions of the electrolyte KCl (0-10 osm) reduced the hypocotyl Px transiently (biphasic response), while the Px reduction by mannitol solutions was sustained. The amplitudes of the induced Px reduction depended directly, and the degree of Px restoration after stress release depended indirectly, on the size of the initial positive Px indicating that mannitol released the root pressure by a mechanical rather than osmotic mechanism. Mannitol treatment and other means of root pressure reduction revealed two separate growth responses in the affected cucumber hypocotyls. Only steep Px drops (following root excision or root pressure release in mannitol) directly cause a rapid, transient drop in growth rate (GR). Both rapid and slow (after root incubation in KCN or NEM) decreases in root pressure, however, led to a sustained growth inhibition of cucumber hypocotyls after about 30 min. This delay characterizes the growth response as an indirect consequence of the Px change. Pea seedlings, which lacked root pressure and had a negative Px throughout, showed extremely small changes in epicotyl Px and GR after root incubation in mannitol. It is apparent that the higher sensitivity of cucumber growth to mannitol depended on the presence and release of root pressure.
Stahlberg
R
R
Department of Biology, Pennsylvania State University, University Park 16802, USA.
Cosgrove
D J
DJ
D001693:Biological Transport, Active; D018553:Cucumis sativus / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D006874:Hydrostatic Pressure; D018546:Hypocotyl / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D008353:Mannitol / Q000494:pharmacology*; D018532:Pisum sativum / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D018520:Plant Shoots / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D011189:Potassium Chloride / Q000494:pharmacology; D014867:Water / Q000378:metabolism*
D014867:Water; D008353:Mannitol; D011189:Potassium Chloride