title: Variations of the effect of insulin on neutrophil respiratory burst. The role of tyrosine kinases and phosphatases.
abstract: The priming effect of insulin on the fMLP-induced respiratory burst of mouse neutrophils as well as the involvement of tyrosine protein kinases and phosphatases in this process have been studied. Peritoneal evoked neutrophils of NMRI strain mice were incubated with 0.01-100 nM insulin for 1-60 min at 22, 30, or 37 degrees C and activated by 0.1-50 microM N-formyl-methionyl-leucyl-phenylalanine (fMLP). The production of reactive oxygen species (ROS) by neutrophils was monitored by luminol-dependent chemiluminescence. We found that 125I-labeled insulin binding by mouse neutrophils occurred with saturation and high affinity. Insulin itself did not change the basal level of the ROS production but could modulate fMLP-induced respiratory burst. The effect of insulin depended on temperature and duration of pretreatment of the neutrophils with insulin and the concentration combination of the insulin and fMLP. The tyrosine kinase inhibitor tyrphostin 51 decreased the fMLP-induced respiratory burst significantly. Insulin did not change the fMLP response of neutrophils pretreated with tyrphostin. However, the effect of tyrphostin on the response to 50 microM fMLP was considerably decreased in neutrophils treated with insulin. There was no such effect during activation by 5 microM fMLP, for which the priming effect of insulin was not observed. Insulin did not increase the fMLP-induced respiratory burst in neutrophils treated with the protein phosphatase inhibitors orthovanadate and pyrophosphate. If the inhibitors were added after insulin, the combined effect was nearly additive. It is possible that priming by insulin of the fMLP-induced respiratory burst is triggered by tyrosine phosphorylation, realized with its participation, and involves the signaling pathways initiated by tyrosine phosphorylation but subsequently is not dependent on the latter. The role of protein phosphatases in priming by insulin is of little importance. The data indirectly confirm the idea that priming of the neutrophil respiratory burst is a result of crosstalk of signaling pathways of the insulin and fMLP receptors with the participation of tyrosine phosphorylation.
authors.affiliation: Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, 142290 Russia. safronova@icb.psn.ru
authors.forename: V G
authors.initials: VG
authors.lastname: Safronova
authors.forename: A G
authors.initials: AG
authors.lastname: Gabdoulkhakova
authors.forename: A V
authors.initials: AV
authors.lastname: Miller
authors.forename: I V
authors.initials: IV
authors.lastname: Kosarev
authors.forename: R N
authors.initials: RN
authors.lastname: Vasilenko
mesh_terms: D000818:Animals; D001665:Binding Sites; D002633:Chemotaxis / Q000502:physiology; D007328:Insulin / Q000378:metabolism* / Q000494:pharmacology; D008163:Luminescent Measurements; D008297:Male; D051379:Mice; D009504:Neutrophils / Q000378:metabolism; D010455:Peptides / Q000378:metabolism*; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism; D016897:Respiratory Burst / Q000187:drug effects / Q000502:physiology*
chemical_list: D007328:Insulin; D010455:Peptides; D017382:Reactive Oxygen Species; D011505:Protein-Tyrosine Kinases; D010744:Phosphoric Monoester Hydrolases