title: Solid-state production of lignin peroxidase (LiP) and manganese peroxidase (MnP) by Phanerochaete chrysosporium using steam-exploded straw as substrate.
abstract: In the used media mainly consisting of steam-exploded wheat straw, the straw, which could replace expensive veratryl alcohol, might act not only as nutrient, but also as inducer of lignin enzymes. The activities of the enzymes lignin peroxidase (LiP) and manganese peroxidase (MnP) in solid-state fermentation (SSF) were far higher than in submerged fermentation (SmF). Under optimal conditions of SSF, the maximum activities of the enzymes Lip and MnP were 2600 and 1375 U/L, respectively. Thus, this would pave the way for production and application of lignin enzymes on a large scale.
authors.affiliation: State Key Laboratory of Biochemical Engineering, Institute of Chemical metallurgy, Chinese Academy of Sciences, Beijing.
authors.forename: X
authors.initials: X
authors.lastname: Fujian
authors.forename: C
authors.initials: C
authors.lastname: Hongzhang
authors.forename: L
authors.initials: L
authors.lastname: Zuohu
mesh_terms: D001592:Benzyl Alcohols / Q000378:metabolism; D002482:Cellulose / Q000378:metabolism*; D005285:Fermentation / Q000502:physiology; D006863:Hydrogen-Ion Concentration; D008031:Lignin / Q000378:metabolism*; D010544:Peroxidases / Q000096:biosynthesis*; D020075:Phanerochaete / Q000201:enzymology*; D013379:Substrate Specificity; D013696:Temperature
chemical_list: D001592:Benzyl Alcohols; D002482:Cellulose; D008031:Lignin; D010544:Peroxidases; C042858:lignin peroxidase; C051129:manganese peroxidase; C042197:veratryl alcohol