title: 5S ribosomal ribonucleic acid sequences in Bacteroides and Fusobacterium:  evolutionary relationships within these genera and among eubacteria in general.
abstract: The 5S ribosomal ribonucleic acid (rRNA) sequences were determined for Bacteroides fragilis, Bacteroides thetaiotaomicron, Bacteroides capillosus, Bacteroides veroralis, Porphyromonas gingivalis, Anaerorhabdus furcosus, Fusobacterium nucleatum, Fusobacterium mortiferum, and Fusobacterium varium.  A dendrogram  constructed by a clustering algorithm from these sequences, which were aligned with all other hitherto known eubacterial 5S rRNA sequences, showed differences as well as similarities with respect to results derived from 16S rRNA analyses.  In the 5S rRNA dendrogram, Bacteroides clustered together with Cytophaga and Fusobacterium, as in 16S rRNA analyses.  Intraphylum relationships deduced from 5S rRNAs suggested that Bacteroides is specifically related to Cytophaga rather than to Fusobacterium, as was suggested by 16S rRNA analyses.  Previous taxonomic considerations concerning the genus Bacteroides, based on biochemical and physiological data, were confirmed by the 5S rRNA sequence analysis.
authors.affiliation: Departement Biochemie, Universiteit Antwerpen, Antwerp, Belgium.
authors.forename: H
authors.initials: H
authors.lastname: Van den Eynde
authors.forename: R
authors.initials: R
authors.lastname: De Baere
authors.forename: H N
authors.initials: HN
authors.lastname: Shah
authors.forename: S E
authors.initials: SE
authors.lastname: Gharbia
authors.forename: G E
authors.initials: GE
authors.lastname: Fox
authors.forename: J
authors.initials: J
authors.lastname: Michalik
authors.forename: Y
authors.initials: Y
authors.lastname: Van de Peer
authors.forename: R
authors.initials: R
authors.lastname: De Wachter
mesh_terms: D000465:Algorithms; D001439:Bacteroides / Q000145:classification / Q000235:genetics*; D001483:Base Sequence; D005075:Biological Evolution; D003589:Cytophaga / Q000145:classification / Q000235:genetics*; D005673:Fusobacterium / Q000145:classification / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012329:RNA, Bacterial / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000032:analysis / Q000737:chemistry / Q000235:genetics; D012341:RNA, Ribosomal, 5S / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
chemical_list: D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012341:RNA, Ribosomal, 5S