title: Growth protocols for etiolated soybeans germinated within BRIC-60 canisters under spaceflight conditions.
abstract: As part of the GENEX (Gene Expression) spaceflight experiment, protocols were developed to optimize the inflight germination and subsequent growth of 192 soybean (Glycine max cv McCall) seeds during STS-87. We describe a method which provided uniform growth and development of etiolated seedlings while eliminating root and shoot restrictions for short-term (4-7 day) experiments. Final seedling growth morphologies and the gaseous CO2 and ethylene levels present both on the last day in space and at the time of recovery within the spaceflight and ground control BRIC-60 canisters are presented.
authors.affiliation: Gravitational Biology Lab., Dynamac Corp., Kennedy Space Center, FL 32899, USA.
authors.forename: H G
authors.initials: HG
authors.lastname: Levine
authors.forename: J A
authors.initials: JA
authors.lastname: Sharek
authors.forename: K M
authors.initials: KM
authors.lastname: Johnson
authors.forename: E C
authors.initials: EC
authors.lastname: Stryjewski
authors.forename: V I
authors.initials: VI
authors.lastname: Prima
authors.forename: O I
authors.initials: OI
authors.lastname: Martynenko
authors.forename: W C
authors.initials: WC
authors.lastname: Piastuch
mesh_terms: D002245:Carbon Dioxide / Q000378:metabolism; D018548:Cotyledon / Q000254:growth & development; D004780:Environment, Controlled; D004867:Equipment Design; D005030:Ethylenes / Q000032:analysis; D018525:Germination; D018546:Hypocotyl / Q000254:growth & development; D010937:Plant Growth Regulators / Q000032:analysis; D018517:Plant Roots / Q000254:growth & development; D012639:Seeds / Q000254:growth & development; D013025:Glycine max / Q000254:growth & development*; D013026:Space Flight / Q000295:instrumentation*; D014893:Weightlessness
chemical_list: D005030:Ethylenes; D010937:Plant Growth Regulators; D002245:Carbon Dioxide; C036216:ethylene