title: Structural characterisation of flavonoids and flavonoid-O-glycosides extracted from Genista tenera by fast-atom bombardment tandem mass spectrometry.
abstract: Genista tenera is a plant native to the Madeira Island (Portugal). From the ethanol extract of its powdered aerial parts, two flavones, three isoflavones and one 7-O-glucosyl isoflavone were isolated. A mass spectrometric study of these compounds was performed using liquid secondary ion mass spectrometry (LSIMS) in combination with high-energy collision-induced dissociation (CID) and tandem mass spectrometry (MS/MS). Characteristic fragmentation patterns were observed in all the investigated compounds; the loss of small neutral species from the protonated molecules was useful for identifying the presence of specific functional groups in the A- and B-rings. In order to help to establish the proposed structures, NMR and UV studies were also performed.
authors.affiliation: Center for Mass Spectrometry, Department of Chemistry and Biochemistry, Faculty of Sciences, University of Lisbon, Edifício C8-2o Piso, Campo Grande, 1749-016 Lisbon, Portugal.
authors.forename: C
authors.initials: C
authors.lastname: Borges
authors.forename: P
authors.initials: P
authors.lastname: Martinho
authors.forename: A
authors.initials: A
authors.lastname: Martins
authors.forename: A P
authors.initials: AP
authors.lastname: Rauter
authors.forename: M A
authors.initials: MA
authors.lastname: Ferreira
mesh_terms: D005419:Flavonoids / Q000032:analysis*; D006027:Glycosides / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D010946:Plants, Medicinal / Q000737:chemistry*; D011174:Portugal; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013056:Spectrophotometry, Ultraviolet
chemical_list: D005419:Flavonoids; D006027:Glycosides