Light-emitting diode treatment reverses the effect of TTX on cytochrome oxidase in neurons.
Light close to and in the near-infrared range has documented benefits for promoting wound healing in human and animals. However, mechanisms of its action on cells are poorly understood. We hypothesized that light treatment with a light-emitting diode array at 670 nm (LED) is therapeutic in stimulating cellular events involving increases in cytochrome oxidase activity. LED was administered to cultured primary neurons whose voltage-dependent sodium channels were blocked by tetrodotoxin. The down-regulation of cytochrome oxidase activity by TTX was reverted to control levels by LED. LED alone also up-regulated enzyme activity. Thus, the results are consistent with our hypothesis that LED has a stimulating effect on cytochrome oxidase in neurons, even when they have been functionally silenced by TTX.
Wong-Riley
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Department of Cell Biology, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA.
Bai
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Buchmann
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Whelan
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D000375:Aging / Q000502:physiology / Q000528:radiation effects; D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured / Q000187:drug effects / Q000201:enzymology / Q000528:radiation effects*; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation / Q000502:physiology / Q000528:radiation effects*; D003576:Electron Transport Complex IV / Q000378:metabolism / Q000528:radiation effects*; D007259:Infrared Rays / Q000627:therapeutic use*; D009410:Nerve Degeneration / Q000139:chemically induced / Q000201:enzymology / Q000517:prevention & control; D009474:Neurons / Q000187:drug effects / Q000201:enzymology / Q000528:radiation effects*; D010775:Photic Stimulation / Q000295:instrumentation* / Q000379:methods; D051381:Rats; D026941:Sodium Channel Blockers; D015222:Sodium Channels / Q000378:metabolism; D013779:Tetrodotoxin / Q000494:pharmacology; D014793:Visual Cortex / Q000187:drug effects / Q000201:enzymology / Q000528:radiation effects; D014945:Wound Healing / Q000502:physiology / Q000528:radiation effects*
D026941:Sodium Channel Blockers; D015222:Sodium Channels; D013779:Tetrodotoxin; D003576:Electron Transport Complex IV