Ultrastructural study of Chlamydia pneumoniae in a continuous-infection model.
We have established an in vitro model of long-term continuous Chlamydia pneumoniae infection in HEp-2 cells. Using transmission electron microscopy, we demonstrated the presence of spontaneous abnormal chlamydial inclusions similar in appearance to the persistent chlamydial forms induced in vitro by treatment with cytokines or antibiotics or by nutrient deprivation.
Kutlin
A
A
Department of Pediatrics, State University of New York Downstate Medical Center, Brooklyn, New York 11203-2098, USA.
Flegg
C
C
Stenzel
D
D
Reznik
T
T
Roblin
P M
PM
Mathews
S
S
Timms
P
P
Hammerschlag
M R
MR
D023521:Chlamydophila Infections / Q000382:microbiology*; D016993:Chlamydophila pneumoniae / Q000472:pathogenicity / Q000502:physiology / Q000648:ultrastructure*; D006801:Humans; D008854:Microscopy, Electron; D014407:Tumor Cells, Cultured