Influence of selenium on mercuric chloride cellular uptake and toxicity indicating protection: studies on cultured K-562 cells.
Selenium and mercuric chloride (MC) interactions regarding cellular uptake and selenium protection on MC toxicity have been studied. Human K-562 cells were pretreated or simultaneously treated with either selenite (5 or 50 microM) or selenomethionine (10 or 50 microM) together with MC (35 or 50 microM). Both treatments with selenite showed an increase of mercury uptake with increased selenium dose. In the pretreated or simultaneously treated selenite and 35 microM MC combinations, no inhibition of growth was seen, whereas all 50-microM MC combinations were toxic to the cells. A selenite-dependent protection was obtained for both exposure protocols when considering the cellular uptake of mercury. The cells died when the accumulation on d 4 reached more than about 0.8 x 10(-15) mol/cell of mercury, whereas they survived up to twofold more mercury uptake when exposed to selenite. Selenomethionine gave, with a few exceptions, similar effects as selenite on MC uptake and toxicity.
Frisk
P
P
Department of Oncology, Radiology and Clinical Immunology, Biomedical Radiation Sciences, Uppsala University, Sweden.
Yaqob
A
A
Nilsson
K
K
Carlsson
J
J
Lindh
U
U
D004305:Dose-Response Relationship, Drug; D006801:Humans; D020014:K562 Cells; D008627:Mercuric Chloride / Q000493:pharmacokinetics* / Q000633:toxicity*; D012643:Selenium / Q000494:pharmacology*; D012645:Selenomethionine / Q000494:pharmacology; D018038:Sodium Selenite / Q000494:pharmacology; D013997:Time Factors
D008627:Mercuric Chloride; D012645:Selenomethionine; D012643:Selenium; D018038:Sodium Selenite