title: Spectrophotometric bioanalytical flow-injection system for control of hemodialysis treatment.
abstract: A spectrophotometric flow-injection analysis (FIA) system for monitoring clinical hemodialysis is demonstrated. The role of a dialysate urea detector incorporated in this bioanalytical system is played by an optical flow-through biosensor based on Prussian Blue film with chemically linked urease forming a monomolecular layer of the enzyme. This pH-enzyme optode-FIA system is useful for the selective determination of post-dialysate urea in the range of concentration corresponding to its level in real clinical samples (2-16 mmol l(-1)). This bioanalytical system allows the analysis of about 15 samples of spent dialysate per hour. The operational and storage stabilities of the applied biosensor are longer than 2 weeks and 2 months, respectively. Clinical evaluation of the bioanalytical system was performed.
authors.affiliation: Department of Chemistry, University of Warsaw, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Radomska
authors.forename: S
authors.initials: S
authors.lastname: Glab
authors.forename: R
authors.initials: R
authors.lastname: Koncki
mesh_terms: D015374:Biosensing Techniques; D015314:Dialysis Solutions / Q000737:chemistry; D006801:Humans; D006435:Renal Dialysis / Q000379:methods*; D013053:Spectrophotometry / Q000379:methods; D014508:Urea / Q000032:analysis
chemical_list: D015314:Dialysis Solutions; D014508:Urea