title: Continuous in vitro propagation of Cowdria ruminantium (Welgevonden stock) in a canine macrophage-monocyte cell line.
abstract: The Welgevonden stock of Cowdria ruminantium, aetiologic agent of heartwater, was continuously propagated in DH82 cells, a continuous canine macrophage-monocyte cell line. Cultures of DH82 cells were readily infected provided that the culture medium was supplemented with cycloheximide. Cultures were split at regular 3-day intervals and infection rates ranged between 60% and 95%. Cultures were continuously propagated through more than 125 passages over a period of more than one year.
authors.affiliation: Onderstepoort Veterinary Institute, Parasitology Division, South Africa.
authors.forename: E
authors.initials: E
authors.lastname: Zweygarth
authors.forename: A I
authors.initials: AI
authors.lastname: Josemans
mesh_terms: D000818:Animals; D000935:Antifungal Agents; D001431:Bacteriological Techniques; D002460:Cell Line; D003470:Culture Media; D003513:Cycloheximide / Q000378:metabolism; D004285:Dogs; D016995:Ehrlichia ruminantium / Q000254:growth & development*; D008264:Macrophages / Q000166:cytology / Q000382:microbiology*; D009000:Monocytes / Q000166:cytology / Q000382:microbiology*; D011500:Protein Synthesis Inhibitors / Q000378:metabolism
chemical_list: D000935:Antifungal Agents; D003470:Culture Media; D011500:Protein Synthesis Inhibitors; D003513:Cycloheximide