title: Quantitative amino acid analysis using a Beckman system gold HPLC 126AA analyzer.
abstract: BACKGROUND
  The Beckman 6300/7300 analyzer, which was widely used for amino acid (AA) analysis, is no longer commercially available.
  
  
  METHODS
  To set up an affordable AA analysis program, a Beckman system gold HPLC 126AA analyzer and Pickering Laboratories reagents were used. Two quantitative AA analysis programs were developed. One was an 18-min short program quantitating seven AAs from plasma and dried blood spots (DBS) specimens using Lithium eluents Li-365 and Li-375 at 70 degrees C column temperature. The short program could be used for diagnosis and follow-up dietary management for phenylketonuria (PKU), maple syrup urine disease (MSUD), tyrosinemia and homocystinuria patients. The second program was a 118-min long AA screening panel quantitating 40 AAs using Lithium eluents Li-275, Li-365 and Li-375 at 32, 48 and 72 degrees C column temperatures from plasma and urine specimens.
  
  
  RESULTS
  The values obtained from DBS specimens were in good agreement with certified results from the Centers for Disease Control and Prevention. The values obtained from plasma and urine samples were in good correlation with those obtained from Beckman 6300 analyzer (0.9076 < or = r < or = 0.999).
  
  
  CONCLUSIONS
  Amino acid quantitation from physiological samples using a Beckman 126AA Analyzer and Pickering Laboratories reagents was useful for clinical diagnosis and monitoring of aminoacidopathies.
authors.affiliation: H.A. Chapman Institute of Medical Genetics, 5300 East Skelly Drive, Tulsa, OK 74135, USA. yong_qu@nymc.edu
authors.forename: Y
authors.initials: Y
authors.lastname: Qu
authors.forename: R H
authors.initials: RH
authors.lastname: Slocum
authors.forename: J
authors.initials: J
authors.lastname: Fu
authors.forename: W E
authors.initials: WE
authors.lastname: Rasmussen
authors.forename: H D
authors.initials: HD
authors.lastname: Rector
authors.forename: J B
authors.initials: JB
authors.lastname: Miller
authors.forename: J G
authors.initials: JG
authors.lastname: Coldwell
mesh_terms: D000596:Amino Acids / Q000097:blood* / Q000652:urine*; D001800:Blood Specimen Collection / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006712:Homocystinuria / Q000175:diagnosis / Q000378:metabolism; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008375:Maple Syrup Urine Disease / Q000175:diagnosis / Q000378:metabolism; D010661:Phenylketonurias / Q000175:diagnosis / Q000378:metabolism; D012984:Software; D020176:Tyrosinemias / Q000175:diagnosis / Q000378:metabolism
chemical_list: D000596:Amino Acids