Human adipose tissue endothelial cells promote preadipocyte proliferation.
Adipogenesis is preceded by development of a microvascular network, and optimal functioning of adipose tissue as an energy store and endocrine organ is dependent on extensive vascularization. We have examined the role of endothelial cell-derived factors that influence the proliferation of human preadipocytes. Microvascular endothelial cells and preadipocytes were isolated from human omental and subcutaneous adipose tissue biopsies by use of a developed procedure of collagenase digest, immunoselection, and differential trypsinization. Conditioned medium from microvascular endothelial cell cultures promoted the proliferation of preadipocytes (P = <0.001) and (to a lesser extent) other cell types. No depot-specific differences in mitogenic capacity of microvascular endothelial cell medium or of preadipocyte response were observed. These results indicate that adipose tissue endothelial cells secrete soluble adipogenic factor(s).
Hutley
L J
LJ
Department of Diabetes and Endocrinology, University of Queensland, Princess Alexandra Hospital, Woolloongabba, Queensland 4102, Australia 4001.
Herington
A C
AC
Shurety
W
W
Cheung
C
C
Vesey
D A
DA
Cameron
D P
DP
Prins
J B
JB
D017667:Adipocytes / Q000166:cytology*; D000273:Adipose Tissue / Q000098:blood supply*; D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal; D001706:Biopsy; D002455:Cell Division; D002469:Cell Separation; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism; D017077:Culture Media, Conditioned; D004730:Endothelium, Vascular / Q000502:physiology*; D005260:Female; D005455:Fluorescent Antibody Technique; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism; D006801:Humans; D008297:Male; D008863:Microspheres; D008875:Middle Aged; D009852:Omentum; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000276:immunology; D013234:Stem Cells / Q000166:cytology*; D014357:Trypsin / Q000378:metabolism
D000911:Antibodies, Monoclonal; D017077:Culture Media, Conditioned; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D003094:Collagen; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D014357:Trypsin