Serotype-specific detection of coxsackievirus A16 in clinical specimens by reverse transcription-nested PCR.
We describe the development of a coxsackievirus A16 (CVA16) serotype-specific PCR which correctly differentiated between CVA16 and other enterovirus serotypes of both laboratory isolates and clinical specimens. The assay will be useful for monitoring CVA16 outbreaks and studying the disease association, epidemiology, and evolution of this common enterovirus serotype.
Bendig
J W
JW
PHLS Coxsackievirus Reference Unit, Epsom, Surrey, United Kingdom. justin.bendig@virgin.net
O'Brien
P S
PS
Muir
P
P
D002213:Capsid / Q000235:genetics; D004770:Enterovirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D004769:Enterovirus Infections / Q000821:virology*; D006801:Humans; D012367:RNA, Viral; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012703:Serotyping; D013045:Species Specificity
D012367:RNA, Viral