Effects of palm oil and transesterified palm oil on chylomicron and VLDL triacylglycerol structures and postprandial lipid response.
The effects of positional distribution of triacylglycerol (TAG) fatty acids to TAG structures in chylomicrons and VLDL, and to postprandial lipemia, were studied in 10 healthy premenopausal women using a 6-h oral fat load test and a randomized, double-blind cross-over design. Molecular level information of TAG regioisomerism was obtained with a tandem mass spectrometric method. The positional distribution of fatty acids in chylomicron TAGs was similar to the respective dietary fat; 79% of the analyzed regioisomers in palm oil and 84% of the analyzed regioisomers in transesterified oil were found in chylomicron TAGs 3 h after the oral fat loads. VLDL TAGs were equal after the two fat loads in all but one regioisomer. Similarities in the fatty acid compositions of chylomicron TAGs suggest that palmitic acid was absorbed equally from both test fats. The proportion of palmitoleic acid in the chylomicrons was increased. Fat with palmitic acid predominantly in the sn-1 and sn-3 positions caused a larger incremental area of total TAGs in plasma and reduced plasma insulin values at the beginning of the postprandial response (0-90 min) compared with fat with palmitic acid randomly distributed. The relationship between TAG molecular structures in dietary fats and in lipoproteins provides new means for understanding the effects of fatty acid positional distribution on human lipid metabolism.
Yli-Jokipii
K
K
Department of Biochemistry and Food Chemistry, University of Turku, FIN-20014 Turku, Finland.
Kallio
H
H
Schwab
U
U
Mykkänen
H
H
Kurvinen
J P
JP
Savolainen
M J
MJ
Tahvonen
R
R
D000328:Adult; D001786:Blood Glucose / Q000378:metabolism; D002784:Cholesterol / Q000097:blood / Q000378:metabolism; D002914:Chylomicrons / Q000097:blood / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D004041:Dietary Fats / Q000494:pharmacology; D004311:Double-Blind Method; D004951:Esterification; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005260:Female; D006801:Humans; D007328:Insulin / Q000097:blood; D008079:Lipoproteins, VLDL / Q000097:blood / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D013058:Mass Spectrometry; D000073878:Palm Oil; D010938:Plant Oils / Q000737:chemistry* / Q000494:pharmacology*; D019518:Postprandial Period / Q000187:drug effects*; D011897:Random Allocation; D013997:Time Factors; D014280:Triglycerides / Q000097:blood / Q000378:metabolism*
D001786:Blood Glucose; D002914:Chylomicrons; D004041:Dietary Fats; D005230:Fatty Acids, Nonesterified; D007328:Insulin; D008079:Lipoproteins, VLDL; D010938:Plant Oils; D014280:Triglycerides; D000073878:Palm Oil; D002784:Cholesterol