Human pancreatic glucokinase (GlkB) complements the glucose signalling defect of Saccharomyces cerevisiae hxk2 mutants.
Human pancreatic glucokinase (GlkB, hexokinase IV) has been expressed in Saccharomyces cerevisiae. The recombinant protein showed similar enzyme kinetics to those described for the original enzyme. When expressed in hxk2 yeast mutants, GlkB complemented both the glucose induction and the glucose repression defects present in the mutant. It was also functional in regulating the activity of the Snf1 kinase complex in response to glucose, participating in the regulation of the Reg1/Glc7 phosphatase complex, as its yeast counterpart.
Mayordomo
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Instituto de Biomedicina de Valencia (CSIC), Jaime Roig 11, 46010 Valencia, Spain.
Sanz
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D005786:Gene Expression Regulation; D005941:Glucokinase / Q000235:genetics* / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006593:Hexokinase / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D009154:Mutation; D010179:Pancreas / Q000201:enzymology; D011994:Recombinant Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics; D015398:Signal Transduction
D011994:Recombinant Proteins; D006593:Hexokinase; D005941:Glucokinase; D005947:Glucose