Pharmacokinetic study on gastrointestinal absorption of insulinomimetic vanadyl complexes in rats by ESR spectroscopy.
Recently, we have shown that oral administrations of vanadyl (+4 oxidation state of vanadium) complexes normalize the blood glucose level of streptozotocin-induced diabetic rats (STZ-rats). To develop clinically useful insulin-mimetic vanadyl complexes, clarification of the pharmacokinetic features of vanadyl compounds is essential. First, we investigated the absorption processes of three compounds, an ionic form of vanadyl sulfate (VS) and the complex forms of bis(picolinato)oxovanadium(IV) (VO(pic)2) and bis(6-methylpicolinato)oxovanadium(IV) (VO(6mpa)2), from the gastrointestinal tract of healthy rats. The concentration curves of paramagnetic vanadyl species in the blood of rats after oral administration of these compounds, as monitored by X-band electron spin resonance (ESR) spectroscopy, exhibited biphasic increasing patterns, indicating that these compounds were absorbed from more than two sites in the gastrointestinal tract. The bioavailability of the compounds was enhanced in the following order on both oral and intraperitoneal administration: VO(6mpa)2 > VO(pic)2 > VS. In addition, bioavailability of the VO(6mpa)2 on ileal administration was enhanced compared with that using other administration sites such as the stomach and jejunum, and resulted in an enhancement about 1.8 fold that compared with oral administration. On the basis of these results, we concluded that the bioavailability of the complex is enhanced most effectively by delivery of the VO(6mpa)2 complex to the ileum.
Fugono
J
J
Department of Analytical and Bioinorganic Chemistry, Kyoto Pharmaceutical University, Japan.
Yasui
H
H
Sakurai
H
H
D000284:Administration, Oral; D000818:Animals; D001682:Biological Availability; D004578:Electron Spin Resonance Spectroscopy; D007004:Hypoglycemic Agents / Q000493:pharmacokinetics* / Q000494:pharmacology; D007263:Infusions, Parenteral; D007328:Insulin / Q000493:pharmacokinetics* / Q000494:pharmacology; D007408:Intestinal Absorption; D007583:Jejunum / Q000502:physiology; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013270:Stomach / Q000502:physiology; D014638:Vanadates / Q000493:pharmacokinetics* / Q000494:pharmacology
D007004:Hypoglycemic Agents; D007328:Insulin; D014638:Vanadates