Effects of a hemoglobin-based oxygen carrier (HBOC-201) on coagulation testing.
Polymerized bovine hemoglobin (HBOC-201) is currently under investigation as an alternative to blood banked human red cells. Due to the dark red, hemolyzed appearance of HBOC-201, we sought to describe the effects of HBOC-201 on coagulation analyzers that perform prothrombin times (PT), activated partial thromboplastin times, fibrinogen, and antithrombin. Pooled normal plasma was combined with HBOC-201 to achieve plasma hemoglobin levels of 1.4, 2.6 3.8, 4.8, and 6.2 g/dL. Results for each test from HBOC-201 prepared plasmas were compared to saline matched controls. Two consecutive absolute result differences of > 10% between saline controls and HBOC-201 samples were used for determining interference on test accuracy by the concentration of HBOC-201. Mechanical detection methods (fibrometer, STA, CS-190) and the MDA-180 were less affected by increasing levels of HBOC-201 than optical detection devices for all test parameters.
Jahr
J S
JS
Department of Anesthesiology, University of California Davis Medical Center, 4150 V Street, Suite 1200, Sacramento, CA 95817, USA.
Lurie
F
F
Gosselin
R
R
Lin
J S
JS
Wong
L
L
Larkin
E
E
D001780:Blood Coagulation Tests; D001802:Blood Substitutes / Q000494:pharmacology*; D006454:Hemoglobins / Q000494:pharmacology*; D006801:Humans
D001802:Blood Substitutes; D006454:Hemoglobins; C096798:HBOC 201