Total internal reflected resonance light scattering determination of chlortetracycline in body fluid with the complex cation of chlortetracycline-europium-trioctyl phosphine oxide at the water/tetrachloromethane interface.
A highly selective method of chlortetracycline (CTC) is proposed on the basis of the measurements of total internal reflected resonance light scattering (TMR-RLS) at water/tetrachloromethane (H20/CCl4) interfaces. In the pH range of 7.54-8.14, the interaction of the binary complex of Eu(III)/CTC in the presence of trioctyl phosphine oxide (TOPO) occurs at the H20/CCl4 interface, resulting in greatly enhanced TIR-RLS signals with the maximum peak located at 340 nm. The enhanced TIR-RLS intensity is in proportion to the CTC concentration in the range 0.98 to approximately 20.0 x 10(-7) mol/L. The limit of detection is 9.8 x 10(-9) mol/L. Synthetic samples and body fluid samples including human urine, human serum, and fresh milk were determined with the recovery of 95.4-106.4% and RSD of 2.9-3.9%.
Feng
P
P
Institute of Environmental Chemistry, Southwest Normal University, Chongqing, People's Republic of China.
Shu
W Q
WQ
Huang
C Z
CZ
Li
Y F
YF
D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000097:blood / Q000652:urine; D002251:Carbon Tetrachloride / Q000032:analysis; D002417:Cattle; D002751:Chlortetracycline / Q000032:analysis* / Q000097:blood / Q000652:urine; D005063:Europium / Q000032:analysis; D006801:Humans; D008027:Light; D008892:Milk / Q000737:chemistry; D009943:Organophosphorus Compounds; D012542:Scattering, Radiation; D014867:Water / Q000032:analysis
D000900:Anti-Bacterial Agents; D009943:Organophosphorus Compounds; D014867:Water; D005063:Europium; C044965:trioctyl phosphine oxide; D002251:Carbon Tetrachloride; D002751:Chlortetracycline