A normalized plot as a novel and time-saving tool in complex enzyme kinetic analysis.
A new data treatment is described for designing kinetic experiments and analysing kinetic results for multi-substrate enzymes. Normalized velocities are plotted against normalized substrate concentrations. Data are grouped into n + 1 families across the range of substrate or product tested, n being the number of substrates plus products assayed. It has the following advantages over traditional methods: (1) it reduces to less than a half the amount of data necessary for a proper description of the system; (2) it introduces a self-consistency checking parameter that ensures the 'scientific reliability' of the mathematical output; (3) it eliminates the need for a prior knowledge of Vmax; (4) the normalization of data allows the use of robust and fuzzy methods suitable for managing really 'noisy' data; (5) it is appropriate for analysing complex systems, as the complete general equation is used, and the actual influence of effectors can be typified; (6) it is amenable to being implemented as a software that incorporates testing and electing among rival kinetic models.
Bravo
I G
IG
Departamento de Bioquímica y Biología Molecular, Universidad de León, Campus Vegazana, Spain.
Busto
F
F
De Arriaga
D
D
Ferrero
M A
MA
Rodríguez-Aparicio
L B
LB
Martínez-Blanco
H
H
Reglero
A
A
D004798:Enzymes / Q000378:metabolism*; D007700:Kinetics; D008956:Models, Chemical; D015233:Models, Statistical
D004798:Enzymes