Decay-accelerating factor (DAF/CD55) is a functional active element of the LPS receptor complex.
Previously, we identified an 80 kDa membrane protein (LMP80) that is capable of binding to LPS and lipid A in the presence of LBP and sCD14. LMP80 could also be detected after immuno-coprecipitation of cell membranes with LPS and lipid A, indicating a physical contact of LMP80 and LPS/lipid A. Further analysis and peptide sequencing revealed that LMP80 is identical to CD55 (decay accelerating factor, DAF), a regulatory molecule of the complement cascade. Transfection of LPS-hyporesponsive Chinese hamster ovary (CHO) cells with human CD55 resulted in the translocation of NF-B upon stimulation with LPS or lipid A. Our results demonstrate a new functional role of CD55 as a molecule able to mediate LPS-induced activation of cells that may be part of a multimeric LPS receptor complex.
Heine
H
H
Center for Medicine and Biosciences, Research Center Borstel, Borstel, Germany.
Ulmer
A J
AJ
El-Samalouti
V T
VT
Lentschat
A
A
Hamann
L
L
D000818:Animals; D001692:Biological Transport; D018958:CD55 Antigens / Q000145:classification / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D016466:CHO Cells; D006224:Cricetinae; D018950:Lipopolysaccharide Receptors / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism; D014162:Transfection
D018958:CD55 Antigens; D018950:Lipopolysaccharide Receptors; D016328:NF-kappa B