Enhanced absorption of 3-O-methyl-D-glucose through the small intestine of rats administered retinyl palmitate.
All-trans retinyl palmitate (RP) (1000 IU/kg body weight) was orally administered to rats for three days. The absorption of 3-O-methyl-D-glucose (3-OMG), which is actively transported by Na+-dependent D-glucose co-transporter (SGLT1), in the small intestine of the control and RP-treated rats was investigated by the in vito everted sac and in situ closed loop of intestine techniques. The absorption of [3H]3-OMG in both experiments of the in vito everted sac and in situ closed loop of intestine significantly increased in the RP-treated rats. AUC(0-120min) obtained from the [3H]3-OMG plasma concentration vs. time curve in the RP-treated rats was significantly larger than that in the control rats. On the other hand, the activity of Na+-K+-adenosinetriphosphatase (ATPase) and the transport rate of D-glucose mediated by Na+-independent facilitative glucose transporter (GLUT2) on the basolateral membrane (BLM) were similar between the control and RP-treated rats. Thus it is suggested that RP treatment of rats enhance the small intestinal absorption of glucose mediated by SGLT1.
Tomimatsu
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Laboratory of Biopharmaceutics, Faculty of Pharmaceutical Sciences, Chiba University, Japan.
Horie
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D019325:3-O-Methylglucose / Q000097:blood / Q000493:pharmacokinetics*; D000284:Administration, Oral; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D019540:Area Under Curve; D001835:Body Weight / Q000187:drug effects; D004224:Diterpenes; D007408:Intestinal Absorption / Q000187:drug effects*; D008297:Male; D008562:Membrane Glycoproteins / Q000502:physiology; D009004:Monosaccharide Transport Proteins / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D000084562:Retinyl Esters; D051269:Sodium-Glucose Transporter 1; D014801:Vitamin A / Q000031:analogs & derivatives* / Q000494:pharmacology*
D000975:Antioxidants; D004224:Diterpenes; D008562:Membrane Glycoproteins; D009004:Monosaccharide Transport Proteins; D000084562:Retinyl Esters; C495676:Slc5a1 protein, rat; D051269:Sodium-Glucose Transporter 1; D014801:Vitamin A; D019325:3-O-Methylglucose; C014794:retinol palmitate