title: Identification of rod- and cone-specific phosducins in teleost retinas.
abstract: Phosducin (PD) is a regulatory protein of vertebrate phototransduction cascades. In mammalian retina, it has been thought that only one kind of PD commonly exists in both rods and cones. However, we have found two kinds of PD (OIPD-R and OIPD-C) in the retina of a teleost, medaka (Oryzias latipes). In situ hybridization and immunohistochemical analysis demonstrated that OIPD-R and -C are selectively expressed in rods and cones, respectively. The antiserum against medaka PDs recognized two kinds of proteins in bluegill (Lepomis macrochirus) retina. These results suggest that rod- and cone-specific PDs exist in teleost retinas, probably creating differences in light adaptation between rods and cones.
authors.affiliation: Department of Earth and Space Science, Graduate School of Science, Osaka University, Toyonaka, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Kobayashi
authors.forename: O
authors.initials: O
authors.lastname: Hisatomi
authors.forename: T
authors.initials: T
authors.lastname: Satoh
authors.forename: F
authors.initials: F
authors.lastname: Tokunaga
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D002417:Cattle; D003001:Cloning, Molecular; D017931:DNA Primers / Q000737:chemistry; D005136:Eye Proteins / Q000235:genetics* / Q000378:metabolism; D020555:GTP-Binding Protein Regulators; D007124:Immunoenzyme Techniques; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D009990:Oryzias; D010473:Perciformes; D010750:Phosphoproteins / Q000235:genetics* / Q000378:metabolism; D020419:Photoreceptor Cells, Vertebrate / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D011494:Protein Kinases / Q000235:genetics* / Q000378:metabolism; D015347:RNA Probes; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017386:Sequence Homology, Amino Acid
chemical_list: D017931:DNA Primers; D005136:Eye Proteins; D020555:GTP-Binding Protein Regulators; D010750:Phosphoproteins; D015347:RNA Probes; D012333:RNA, Messenger; C065631:phosducin; D011494:Protein Kinases