Analysis of salivary gland proteins of the mosquito Anopheles darlingi (Diptera: Culicidae).
The salivary proteins of Anopheles darlingi Root, the principal vector of malaria in the Amazon Region, Brazil, were analyzed. Comparison of the protein profiles between adult males and females revealed that most of the polypeptides are present in both sexes, but female-specific polypeptides also were observed. SDS-PAGE analysis of sugar-fed female mosquitoes with ages varying from 1 to 10 d after adult emergence indicated that the proteins start to be accumulated in the first day of life and are present throughout the period analyzed. Analysis of blood-fed mosquitoes showed no differences in salivary proteins when compared with sugar fed ones, suggesting that there is no specific protein induced by blood. The protein profiles of the salivary glands dissected from wild-caught female mosquitoes from different geographical regions of Brazil were compared and some differences were observed.
Moreira
C K
CK
Departamento de Parasitologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, SP, Brazil.
Marrelli
M T
MT
Lima
S P
SP
Marinotti
O
O
D000818:Animals; D000852:Anopheles / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005260:Female; D019476:Insect Proteins / Q000032:analysis*; D008297:Male; D010455:Peptides / Q000032:analysis; D012469:Salivary Glands / Q000737:chemistry*
D019476:Insect Proteins; D010455:Peptides