Sweetness and enzymatic activity of lysozyme.
Hen egg lysozyme elicits a sweet taste sensation for human beings. Effects of reduction of disulfide bonds, heat treatment, and chemical modification of hen egg lysozyme on both sweetness and hydrolytic activity were investigated. Both the sweetness and enzymatic activities were lost when the intradisulfide linkage in a lysozyme molecule was reduced and S-3-(trimethylated amino) propylated. The sweetness and enzymatic activity of lysozyme were lost on heating at 95 degrees C for 18 h. These facts suggest that tertiary structures of lysozyme are indispensable for eliciting a sweet taste as well as enzymatic activity. Although the modification of carboxyl residues in a lysozyme by glycine methylester or aminomethansulfonic acid resulted in the loss of enzymatic activity by blocking the catalytic residues, the sweetness was fully retained. These results indicate that the sweetness of lysozyme was independent of its enzymatic activity. The lysozyme purified from goose egg white similarly elicited a sweet taste, although goose (g-type) lysozyme is quite different from hen egg lysozyme (c-type) on the basis of structural, immunological, and enzymatic properties. These findings indicate that a specific protein property of lysozyme is required for sweetness elicitation and that the enzymatic activity and carbohydrates produced by enzymatic reaction are not related to the sweet taste.
Masuda
T
T
Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611-0011, Japan.
Ueno
Y
Y
Kitabatake
N
N
D000818:Animals; D002645:Chickens; D004220:Disulfides / Q000737:chemistry; D004529:Egg White / Q000032:analysis; D005260:Female; D005777:Geese; D005998:Glycine / Q000031:analogs & derivatives* / Q000737:chemistry; D006358:Hot Temperature; D006801:Humans; D009113:Muramidase / Q000737:chemistry / Q000378:metabolism*; D010084:Oxidation-Reduction; D013329:Structure-Activity Relationship; D020310:Struthioniformes; D013451:Sulfonic Acids / Q000737:chemistry; D013649:Taste
D004220:Disulfides; D013451:Sulfonic Acids; C035591:glycine methyl ester; D009113:Muramidase; C045904:aminomethanesulfonic acid; D005998:Glycine