IL-12 and IL-18 synergistically induce the bactericidal activity of murine peritoneal cells against M. leprae.
We examined the effect of IL-12 and IL-18 on bactericidal activities of mouse peritoneal cell (PC) against Mycobacterium leprae (M. leprae). We demonstrated that IL-12 and IL-18 synergistically induced the NO-dependent bactericidal activity of PC by stimulating Natural Killer (NK) cells and T-cells through IFN-gamma production. IL-12 and IL-18 induced host cell death through NK-cells and T-cells. Therefore. IL-12 and IL-18 play an important role on direct killing of intracellular M. leprae and on indirect killing of them through inducing host cell death.
Nomaguchi
H
H
Leprosy Research Center, National Institute Infectious Diseases, 4-2-1 Aobacho, Higashimurayama-shi, Tokyo 189-0002, Japan. h-nomagc@nih.go.jp
Jahan
N
N
Mandal
B C
BC
Yogi
Y
Y
Kawatsu
K
K
Yoshizawa
Y
Y
Okamura
H
H
Makino
M
M
D000818:Animals; D002478:Cells, Cultured; D004357:Drug Synergism; D005260:Female; D007371:Interferon-gamma / Q000378:metabolism; D018664:Interleukin-12 / Q000494:pharmacology* / Q000502:physiology; D020382:Interleukin-18 / Q000494:pharmacology* / Q000502:physiology; D007694:Killer Cells, Natural / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009166:Mycobacterium leprae / Q000276:immunology*; D009569:Nitric Oxide / Q000378:metabolism; D010529:Peritoneal Cavity / Q000166:cytology*; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism
D020382:Interleukin-18; D018664:Interleukin-12; D009569:Nitric Oxide; D007371:Interferon-gamma