title: Cloning of the red kangaroo (Macropus rufus) follicle stimulating hormone beta subunit.
abstract: The cDNA encoding the follicle stimulating hormone beta subunit (FSH-beta) was isolated from a red kangaroo pituitary cDNA library by using a porcine probe and the nucleotide sequence for the coding region was determined. The highest degree of deduced amino acid sequence identity (91%) was observed between the red kangaroo and another marsupial, the brushtail possum (Trichosurus vulpecula), followed by eutherian species (76%, 75% and 74%, respectively, for pig, mouse and sheep). Based on the deduced red kangaroo FSH-beta amino acid sequence, putative antigenic sites have been identified that may prove useful for studying the hormonal control of reproduction in marsupials.
authors.affiliation: CRC for Conservation and Management of Marsupials, School of Biological Sciences, Macquarie University, North Ryde NSW, Australia.
authors.forename: K
authors.initials: K
authors.lastname: Belov
authors.forename: G A
authors.initials: GA
authors.lastname: Harrison
authors.forename: D W
authors.initials: DW
authors.lastname: Cooper
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000302:isolation & purification; D005640:Follicle Stimulating Hormone / Q000235:genetics*; D037201:Follicle Stimulating Hormone, beta Subunit; D015723:Gene Library; D007614:Macropodidae / Q000235:genetics*; D008969:Molecular Sequence Data; D009893:Opossums; D010902:Pituitary Gland / Q000737:chemistry; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D018076:DNA, Complementary; D037201:Follicle Stimulating Hormone, beta Subunit; D005640:Follicle Stimulating Hormone