Molecular identification of Candida parapsilosis from crop mucosa in a cockatiel.
A 2-month-old cockatiel was evaluated for diarrhea, dyspnea, and death. Histologic examination of lesions in the crop mucosa revealed hyperkeratosis and the presence of blastoconidia and hyphae. Positive immunohistochemical staining of the organisms was achieved with an antibody directed against Candida spp. Polymerase chain reaction amplification of DNA from crop lesion material with internal transcribed spacer 2 (ITS2) primers yielded fragments of approximately 300 bp, which demonstrated 95% DNA homology with the corresponding sequence from a strain of Candida parapsilosis deposited in the GenBank data base. The Candida species in the lesion of the crop mucosa was therefore identified by DNA sequence analysis as C. parapsilosis.
Kano
R
R
Department of Pathobiology, Nihon University School of Veterinary Medicine, Fujisawa, Kanagawa, Japan.
Sakamoto
Y
Y
Hanahachi
A
A
Kamata
H
H
Fukuda
Y
Y
Fujiwara
K
K
Hasegawa
A
A
D000818:Animals; D002175:Candida / Q000302:isolation & purification* / Q000472:pathogenicity; D002177:Candidiasis / Q000235:genetics / Q000473:pathology / Q000662:veterinary*; D003426:Crop, Avian / Q000382:microbiology* / Q000473:pathology; D004269:DNA, Bacterial / Q000032:analysis; D003967:Diarrhea / Q000209:etiology / Q000662:veterinary; D004417:Dyspnea / Q000209:etiology / Q000662:veterinary; D010311:Parrots / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial