The replication of certain Coxsackie B virus strains in CHO cells.
Cell surface molecules that can act as viral receptors may exert an important selective pressure on RNA viral quasi-species. Coxsackie-Adenovirus Receptor and Decay Accelerating Factor (DAF, CD55) have been identified as receptors for Coxsackie B virus. In studies of viral replication using different strains of Coxsackievirus serotype 4 (CBV-4), it was found that despite lack of expression of these cell surface molecules on Chinese Hamster Ovary (CHO) cells and despite their common use as negative controls in Coxsackie B virus receptor assays, two strains were able to replicate, one (V89-4557) without cytopathic effect (CPE), and the other (T318) with strong CPE. A weak signal was obtained using antibodies against enterovirus, visualized with FITC-conjugated antibodies, when the Coxsackievirus B4 strain V89-4557 was inoculated on CHO cells compared to no signal with the non-replicating Coxsackievirus B4 strain VD2921, indicating some degree of binding of the former to the cells.
Frisk
G
G
Department of Women's and Children's Health, Uppsala University, Akademiska Hospital, S-751 85 Uppsala, Sweden. gun.frisk@kbh.uu.se
Elfström
T
T
Diderholm
H
H
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000378:metabolism*; D018958:CD55 Antigens / Q000378:metabolism; D016466:CHO Cells; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D003588:Cytopathogenic Effect, Viral; D029822:Enterovirus B, Human / Q000145:classification / Q000254:growth & development* / Q000378:metabolism* / Q000502:physiology; D016650:Fluorescein-5-isothiocyanate / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D018960:Hyaluronan Receptors / Q000378:metabolism; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism
D000911:Antibodies, Monoclonal; D018958:CD55 Antigens; D018960:Hyaluronan Receptors; D018799:Intercellular Adhesion Molecule-1; D016650:Fluorescein-5-isothiocyanate