An Arabidopsis SNF1-related protein kinase, AtSR1, interacts with a calcium-binding protein, AtCBL2, of which transcripts respond to light.
AtSR1 is a protein kinase of Arabidopsis thaliana, which belongs to the SNF1-related protein kinase subfamily 3. We previously showed accumulation of its transcripts to be responsive to light. In this study, we examined the interaction between AtSR1 and six calcineurin B like proteins of Arabidopsis and found that AtSR1 prominently interacts with one of them, AtCBL2, by yeast two-hybrid assay. Interaction between AtSR1 and AtCBL2 could also be directly confirmed in vitro by pull down assay. RNA blot and reverse transcription-polymerase chain reaction analyses showed that transcripts of AtCBL2, and also of AtCBL1, another CBL, increased upon illumination of leaves. The physiological meaning of the interaction of AtSR1and AtCBL2 is not clear, but they presumably function in signal transduction of light.
Nozawa
A
A
Research and Education Center for Genetic Information, Nara Institute of Science and Technology, Ikoma, Nara, 630-0101 Japan.
Koizumi
N
N
Sano
H
H
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism; D019703:Calcineurin / Q000145:classification / Q000235:genetics / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D008027:Light; D008969:Molecular Sequence Data; D010802:Phylogeny; D011494:Protein Kinases / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D015398:Signal Transduction; D014158:Transcription, Genetic
D029681:Arabidopsis Proteins; D019703:Calcineurin; D002135:Calcium-Binding Proteins; D011494:Protein Kinases; D017346:Protein Serine-Threonine Kinases; C417306:CBL protein, Arabidopsis; C490996:At5g01820 protein, Arabidopsis