A 4.1-kilodalton polypeptide in the cultural supernatant of Mycoplasma fermentans is one of the substances responsible for induction of interleukin-6 production by human gingival fibroblasts.
The cultural supernatant of Mycoplasma fermentans induced interleukin-6 production by human gingival fibroblasts. The active entities were divided into hydrophilic and hydrophobic substances. In this study, we purified a 4.1-kilodalton polypeptide from the hydrophilic substances. It reacted with polyclonal antibodies to M. fermentans and activated human macrophages.
Hasebe
A
A
Department of Oral Pathobiological Science, Graduate School of Dental Medicine, Hokkaido University, Nishi 7, Kita 13, Kita-ku, Sapporo 060-8586, Japan. akkun@den.hokudai.ac.jp
Shibata
K
K
Watanabe
T
T
D000818:Animals; D001426:Bacterial Proteins / Q000276:immunology* / Q000302:isolation & purification; D002241:Carbohydrates / Q000032:analysis; D002460:Cell Line; D002478:Cells, Cultured; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005347:Fibroblasts / Q000166:cytology / Q000276:immunology; D005881:Gingiva / Q000166:cytology / Q000276:immunology*; D006801:Humans; D007375:Interleukin-1 / Q000032:analysis; D015850:Interleukin-6 / Q000096:biosynthesis*; D008970:Molecular Weight; D016858:Mycoplasma fermentans / Q000276:immunology* / Q000378:metabolism; D010455:Peptides / Q000276:immunology* / Q000302:isolation & purification; D011817:Rabbits; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
D001426:Bacterial Proteins; D002241:Carbohydrates; D003470:Culture Media; D007375:Interleukin-1; D015850:Interleukin-6; D010455:Peptides; D014409:Tumor Necrosis Factor-alpha