title: Tracking of oocyte dysmorphisms for ICSI patients may prove relevant to the outcome in subsequent patient cycles.
abstract: BACKGROUND
  We determined whether oocyte dysmorphisms, especially repetition of specific dysmorphisms from cycle to cycle, had a prognostic impact on intracytoplasmic sperm injection (ICSI) outcome.
  
  
  METHODS
  ICSI patients (n = 67) were grouped as follows: group 1 >50% phenotypically dysmorphic oocytes per cohort (cytoplasmic and extra-cytoplasmic dysmorphisms) with no repetition of a specific dysmorphism from cycle one to cycle two (36 cycles and 274 oocytes); group 2 >50% dysmorphic oocytes per cohort and repetition of the same dysmorphism from cycle one to cycle two (32 cycles and 313 oocytes); group 3 (control) <30% dysmorphic oocytes (33 cycles and 378 oocytes).
  
  
  RESULTS
  In group 2 (repetitive), 47% of oocytes were observed to have organelle clustering versus 20.5% in group 1 and 17.3% in group 3 (P < 0.001). There was no difference between the groups in fertilization rates, cleavage rates or embryo quality. Embryos derived from normal oocytes were transferred in each group (57, 33 and 72% respectively). The clinical pregnancy and implantation rates in group 2 (3.1 and 1.7% respectively) were lower (P < 0.01, P = 0.005) than both group 1 (28 and 15% respectively) and group 3 (45.5 and 26.5% respectively).
  
  
  CONCLUSIONS
  The low implantation rate in group 2, even though 33% of transferred embryos were derived from morphologically normal oocytes, suggests that repetitive organelle clustering may be associated with an underlying adverse factor affecting the entire follicular cohort.
authors.affiliation: Division of Reproductive Sciences, Department of Obstetrics and Gynecology, Toronto Centre for Advanced Reproductive Technology, University of Toronto, Toronto, Ontario, Canada.
authors.forename: J S
authors.initials: JS
authors.lastname: Meriano
authors.forename: J
authors.initials: J
authors.lastname: Alexis
authors.forename: S
authors.initials: S
authors.lastname: Visram-Zaver
authors.forename: M
authors.initials: M
authors.lastname: Cruz
authors.forename: R F
authors.initials: RF
authors.lastname: Casper
mesh_terms: D000328:Adult; D015331:Cohort Studies; D010064:Embryo Implantation; D005260:Female; D006801:Humans; D009865:Oocytes / Q000648:ultrastructure*; D015388:Organelles / Q000648:ultrastructure; D011247:Pregnancy; D018873:Pregnancy Rate; D011379:Prognosis; D012189:Retrospective Studies; D020554:Sperm Injections, Intracytoplasmic; D016896:Treatment Outcome