Distinct mRNA microarray profiles of tolerogenic dendritic cells.
Dendritic cells are crucial to the activation as well as suppression of the immune response. Previous reports have illustrated that APC interacting with antigen-specific T suppressor cells become tolerogenic, inducing T helper anergy. To characterize the molecular changes occurring in tolerogenic APC, the mRNA profile of KG-1 dendritic cells exposed to allospecific T helper and T suppressor cells were analyzed. This study now provides evidence that immature dendritic cells stimulated by T suppressor cells differentiate into mature dendritic cells with a distinct phenotype. The identification of Ts induced pathways of dendritic cell differentiation is critical to the development of new therapeutic strategies.
Suciu-Foca Cortesini
N
N
Department of Pathology, Columbia University, New York, NY 10032, USA. ns20@columbia.edu
Piazza
F
F
Ho
E
E
Ciubotariu
R
R
LeMaoult
J
J
Dalla-Favera
R
R
Cortesini
R
R
D000943:Antigens, Differentiation; D017209:Apoptosis / Q000235:genetics / Q000276:immunology; D002450:Cell Communication / Q000235:genetics / Q000276:immunology; D018797:Cell Cycle Proteins; D002455:Cell Division / Q000235:genetics / Q000276:immunology; D002460:Cell Line; D018925:Chemokines / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D018920:Coculture Techniques; D051546:Cyclooxygenase 2; D016207:Cytokines / Q000096:biosynthesis / Q000235:genetics; D003713:Dendritic Cells / Q000166:cytology / Q000201:enzymology / Q000276:immunology* / Q000378:metabolism; D015536:Down-Regulation / Q000235:genetics / Q000276:immunology; D020869:Gene Expression Profiling / Q000379:methods; D006801:Humans; D007108:Immune Tolerance / Q000235:genetics; D007527:Isoenzymes / Q000096:biosynthesis / Q000235:genetics; D008213:Lymphocyte Activation / Q000235:genetics; D008565:Membrane Proteins; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D011451:Prostaglandin-Endoperoxide Synthases / Q000096:biosynthesis / Q000235:genetics; D014176:Protein Biosynthesis; D054645:Protein Phosphatase 1; D011506:Proteins / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics*; D015398:Signal Transduction / Q000235:genetics / Q000276:immunology; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics; D014407:Tumor Cells, Cultured; D015854:Up-Regulation / Q000235:genetics / Q000276:immunology
D000943:Antigens, Differentiation; D018797:Cell Cycle Proteins; D018925:Chemokines; D016207:Cytokines; D007527:Isoenzymes; D008565:Membrane Proteins; D011506:Proteins; D012333:RNA, Messenger; D014157:Transcription Factors; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; D011451:Prostaglandin-Endoperoxide Synthases; C087519:PPP1R15A protein, human; D054645:Protein Phosphatase 1