title: Chitinase in cucumber xylem sap.
abstract: A chitinase activity was detected in fractions of xylem sap collected from the cut surface of cucumber stems. A 28-kDa acidic protein was purified from the active fractions and its N-terminal amino acid sequence was found to be identical to that of a chitinase gene. Cucumber roots produce and secrete an acidic chitinase, one of the PR proteins, into xylem sap and deliver it to aboveground organs.
authors.affiliation: Noda Institute for Scientific Research, Chiba, Japan. susumu.masuda@nifty.ne.jp
authors.forename: S
authors.initials: S
authors.lastname: Masuda
authors.forename: H
authors.initials: H
authors.lastname: Kamada
authors.forename: S
authors.initials: S
authors.lastname: Satoh
mesh_terms: D000595:Amino Acid Sequence; D002688:Chitinases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D002852:Chromatography, Ion Exchange; D018553:Cucumis sativus / Q000201:enzymology* / Q000235:genetics; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification; D018517:Plant Roots / Q000201:enzymology
chemical_list: D010455:Peptides; D002688:Chitinases