Bioassay of cadmium using a DNA microarray: genome-wide expression patterns of Saccharomyces cerevisiae response to cadmium.
DNA microarray technology enables genome-wide detection of cell response at the transcriptional level. We are planning to make bioassay systems that can detect environmental chemicals to screen for potential bioreactive agents. To develop a DNA microarray for our purposes, the changes in gene expression underlying the yeast stress response to cadmium were analyzed by a microarray of total mRNA. Cadmium is a potent cell poison known to cause oxidative stress by changing intracellular glutathione levels. We report here that not only the glutathione synthesis gene (GSH1) but also almost all transcripts of the enzymes involved in the sulfur amino acid metabolism, especially MET14 and MET17, were greatly induced after exposure to cadmium. While several common stress-responsive genes, such as HSP26, GRE1, HSP12, and DDR48, were up-regulated more than almost fourfold by cadmium, there were also 42 other genes up-regulated more than fourfold. Based on these results, we concluded that DNA microarrays are very useful instruments for creating new bioassay systems and finding genetic promoters of stress indicators.
Momose
Y
Y
Human Stress Signal Research Center and Research Institute of Biological Resources, The National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan.
Iwahashi
H
H
D000222:Adaptation, Physiological; D000603:Amino Acids, Sulfur / Q000378:metabolism; D001483:Base Sequence; D001681:Biological Assay / Q000379:methods; D002104:Cadmium / Q000009:adverse effects*; D004784:Environmental Monitoring / Q000379:methods; D020869:Gene Expression Profiling; D005981:Glutathione Synthase / Q000096:biosynthesis / Q000235:genetics; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D018384:Oxidative Stress; D012333:RNA, Messenger / Q000096:biosynthesis; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics*; D015854:Up-Regulation
D000603:Amino Acids, Sulfur; D012333:RNA, Messenger; D002104:Cadmium; D005981:Glutathione Synthase