Salp25D, an Ixodes scapularis antioxidant, is 1 of 14 immunodominant antigens in engorged tick salivary glands.
Rabbits or guinea pigs infested with Ixodes scapularis acquire resistance to tick bites, a phenomenon, known as tick immunity, that is partially mediated by antibody. To determine the salivary gland antigens that elicit antibodies in the host, an I. scapularis salivary gland cDNA expression library was probed with serum from tick-immune rabbits. Sera from sensitized rabbits strongly recognized 47 of 100,000 library clones in an antibody-screening assay. These 47 clones encoded 14 different I. scapularis genes, including a glutathione peroxidase homologue. Expression of these 14 genes in engorged tick salivary glands was confirmed by reverse-transcription polymerase chain reaction. The I. scapularis glutathione peroxidase homologue, named salp25D, was expressed in both unfed and fed nymphal salivary glands. Recombinant Salp25D was able to catalyze the reduction of hydrogen peroxide in the presence of reduced glutathione and glutathione reductase. These results categorize the prominent salivary gland proteins in I. scapularis and demonstrate the presence of a potent antioxidant in tick saliva.
Das
S
S
Section of Rheumatology, Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut 06520-8031, USA.
Banerjee
G
G
DePonte
K
K
Marcantonio
N
N
Kantor
F S
FS
Fikrig
E
E
D000595:Amino Acid Sequence; D000818:Animals; D000941:Antigens / Q000032:analysis*; D000975:Antioxidants / Q000032:analysis*; D002417:Cattle / Q000276:immunology; D003001:Cloning, Molecular; D016384:Consensus Sequence; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015723:Gene Library; D005979:Glutathione Peroxidase / Q000737:chemistry / Q000235:genetics; D006168:Guinea Pigs / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D019476:Insect Proteins / Q000235:genetics / Q000276:immunology*; D018884:Ixodes / Q000145:classification / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008809:Mice, Inbred C3H; D008969:Molecular Sequence Data; D009348:Nematoda / Q000276:immunology; D012333:RNA, Messenger / Q000235:genetics; D011817:Rabbits / Q000276:immunology; D012469:Salivary Glands / Q000737:chemistry / Q000276:immunology*; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D013997:Time Factors
D000941:Antigens; D000975:Antioxidants; D007074:Immunoglobulin G; D019476:Insect Proteins; D012333:RNA, Messenger; C439814:gph protein, Ixodes scapularis; D005979:Glutathione Peroxidase