title: Chiral analysis of methylphenidate and dextromoramide by capillary electrophoresis.
abstract: Capillary electrophoretic methods have been developed to separate the enantiomers of methylphenidate (MPH) and dextromoramide. For MPH separation was achieved with heptakis (2,6-di-O-methyl)-beta-cyclodextrin (DMCD) as chiral selector in a 100 mM phosphoric acid buffer adjusted to pH 3.0 with triethanolamine. Commercial samples of D,L-erytho-MPH HCl and D,L-threo-MPH HCl were analysed using the method. There was no evidence of the presence of D,L-threo-MPH HCl in D,L-erytho-MPH HCl and vice versa. The ratio of the enantiomers was determined for each diastereoisomer. Hydroxypropyl-beta-cyclodextrin was the chiral selector of choice for the chiral separation of the enantiomers of moramide. The separation which gave a resolution of about 3.5 was achieved in 4 min using only a 6 cm of length of capillary. In a sample of dextro-R-moramide tartrate only a small quantity (4.9% w/w) of levo-S-moramide was detected with this method.
authors.affiliation: Department of Pharmaceutical Sciences, University of Strathclyde, Strathclyde Institute of Biomedical Sciences, Glasgow, Scotland, UK.
authors.forename: O M
authors.initials: OM
authors.lastname: Denk
authors.forename: D G
authors.initials: DG
authors.lastname: Watson
authors.forename: G G
authors.initials: GG
authors.lastname: Skellern
mesh_terms: D002021:Buffers; D003916:Dextromoramide / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods*; D008774:Methylphenidate / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013237:Stereoisomerism
chemical_list: D002021:Buffers; D008774:Methylphenidate; D003916:Dextromoramide