Detection of human gastric cancer in resected specimens using a novel infrared fluorescent anti-human carcinoembryonic antigen antibody with an infrared fluorescence endoscope in vitro.
OBJECTIVE
An indocyanine green derivative (ICG-sulfo-OSu) that can be used as an infrared fluorescent labeling substance suitable for detecting microlesions with an infrared fluorescence endoscope has been developed. The aims of the present study were to develop an infrared fluorescence endoscope and to demonstrate its usefulness in detecting cancerous tissue using an antibody coupled with ICG-sulfo-OSu.


METHODS
ICG-sulfo-OSu-labeled mouse anti-human carcinoembryonic antigen (CEA) antibody and an infrared fluorescence endoscope were used in this study. Biopsy specimens of gastric cancer were stained with anti-CEA antibody using the avidin-biotinylated peroxidase complex method. The positive specimens used for the infrared imaging analysis were freshly resected stomachs from three patients.


RESULTS
Treatment of freshly resected stomach specimens with ICG-sulfo-OSu-labeled-anti-CEA antibody complex resulted in positive staining of the tumor sites on infrared fluorescence endoscopy, and the infrared fluorescent images correlated well with the tumor sites.


CONCLUSIONS
An anti-CEA antibody with affinity for cancerous lesions and labeled with ICG-sulfo-OSu can therefore be imaged using this infrared fluorescence endoscope. Specific antibodies tagged with ICG-sulfo-OSu can label cancer cells and can generate a strong enough fluorescent signal to detect small cancers when examined with an infrared fluorescence endoscope.
Ito
S
S
Second Dept. of Internal Medicine, School of Medicine, University of Tokushima, Kuramoto, Tokushima, Japan. ito@clin.med.tokushima-u.ac.jp
Muguruma
N
N
Kusaka
Y
Y
Tadatsu
M
M
Inayama
K
K
Musashi
Y
Y
Yano
M
M
Bando
T
T
Honda
H
H
Shimizu
I
I
Ii
K
K
Takesako
K
K
Takeuchi
H
H
Shibamura
S
S
D000230:Adenocarcinoma / Q000175:diagnosis* / Q000276:immunology; D000818:Animals; D000911:Antibodies, Monoclonal; D002272:Carcinoembryonic Antigen / Q000276:immunology*; D016099:Endoscopy, Gastrointestinal / Q000379:methods; D005260:Female; D005453:Fluorescence; D005455:Fluorescent Antibody Technique / Q000379:methods; D005456:Fluorescent Dyes; D006801:Humans; D007150:Immunohistochemistry; D007208:Indocyanine Green / Q000031:analogs & derivatives*; D007259:Infrared Rays; D008297:Male; D051379:Mice; D013274:Stomach Neoplasms / Q000175:diagnosis* / Q000276:immunology
D000911:Antibodies, Monoclonal; D002272:Carcinoembryonic Antigen; D005456:Fluorescent Dyes; C113792:indocyanine green-sulfo-OSu; D007208:Indocyanine Green