title: Measurement of bisphenol A levels in human urine.
abstract: We report a new approach for assessing human exposure to bisphenol A (BPA) by measuring BPA in urine after enzymatic deglucuronidation. This method involves addition of (13)C(12)-labeled BPA, enzymatic deconjugation, solid-phase extraction, and derivatization with pentafluorobenzyl bromide. The product of the derivatization is separated by gas chromatography followed by mass spectrometric detection using negative chemical ionization and selected ion monitoring. Using this analysis method, urine samples fortified with both a constant level of labeled BPA and a range of unlabeled BPA levels (0.27-10.6 ng/ml) demonstrated constant percentage recovery. In addition, a range of urine sample volumes (0.25-10.0 ml) with constant amounts of added internal standard produced a linear response (r(2)=0.99). The method limit of detection was 0.12 ng/ml. This method was validated by duplicate analyses using gas chromatography coupled to a high-resolution mass spectrometer.
authors.affiliation: National Center for Environmental Health, Centers for Disease Control and Prevention (CDC), Atlanta, Georgia, USA. jwb6@cdc.gov
authors.forename: J W
authors.initials: JW
authors.lastname: Brock
authors.forename: Y
authors.initials: Y
authors.lastname: Yoshimura
authors.forename: J R
authors.initials: JR
authors.lastname: Barr
authors.forename: V L
authors.initials: VL
authors.lastname: Maggio
authors.forename: S R
authors.initials: SR
authors.lastname: Graiser
authors.forename: H
authors.initials: H
authors.lastname: Nakazawa
authors.forename: L L
authors.initials: LL
authors.lastname: Needham
mesh_terms: D001559:Benzhydryl Compounds; D002247:Carbon Isotopes; D002623:Chemistry Techniques, Analytical / Q000379:methods; D004781:Environmental Exposure; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D010636:Phenols / Q000652:urine*; D012016:Reference Values; D012680:Sensitivity and Specificity; D016482:Urinalysis; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D002247:Carbon Isotopes; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds