title: Antibody response to E2 glycoprotein induced in mice by immunization with plasmid DNA containing sequence derived from a Chinese genotype III/2a isolate of hepatitis C virus.
abstract: OBJECTIVE
  To induce antibody response against E2 glycoprotein derived from a Chinese genotype III/2a isolate of hepatitis C virus (HCV) in BALB/C mice by plasmid DNA-based immunization.
  
  
  METHODS
  Plasmid p3W14 containing E2/NS1 gene derived from a Chinese genotype III/2a isolate of HCV, which was verified to express 70 kDa E2 glycoprotein in NIH3T3 cells, was used for direct intramuscular injection in BALB/C mice. Specific antibody to E2 glycoprotein was detected by recombinant E2 protein.
  
  
  RESULTS
  Specific anti-E2 antibody could be detected in mice inoculated with p3W14(5/6). Titer of antibody ranged from 1:15 to 1:120.
  
  
  CONCLUSIONS
  Successfully inducing anti-E2 antibody in mice by plasmid DNA-based immunization containing E2/NS1 gene from a Chinese genotype III/2a isolate of HCV is the first time in China.
authors.affiliation: Institute of Hepatology, People's Hospital, Beijing Medical University, Beijing 100044, China.
authors.forename: C
authors.initials: C
authors.lastname: Wu
authors.forename: Q
authors.initials: Q
authors.lastname: Tao
authors.forename: B
authors.initials: B
authors.lastname: Feng
mesh_terms: D000071443:12E7 Antigen; D000818:Animals; D000906:Antibodies / Q000097:blood; D015703:Antigens, CD / Q000235:genetics / Q000276:immunology*; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000276:immunology*; D005260:Female; D005838:Genotype; D016174:Hepacivirus / Q000235:genetics*; D006526:Hepatitis C / Q000517:prevention & control* / Q000821:virology; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids / Q000008:administration & dosage / Q000276:immunology*; D011897:Random Allocation; D014611:Vaccination; D019444:Vaccines, DNA
chemical_list: D000071443:12E7 Antigen; D000906:Antibodies; D015703:Antigens, CD; C061828:CD99 protein, human; D015815:Cell Adhesion Molecules; D019444:Vaccines, DNA