Contamination of transplantable human tumor-bearing lines by Helicobacter hepaticus and its elimination.
Helicobacter hepaticus contaminating nonfrozen tumors was transmissible to severe combined immunodeficient (SCID) mice, but the organism in cryopreserved samples was not. This suggests that H. hepaticus has the ability to spread via biomaterials and that freezing-thawing is able to reduce the numbers of organisms to levels insufficient for subcutaneous infection of SCID mice.
Goto
K
K
Central Institute for Experimental Animals, Miyamae-ku, Kawasaki, Kanagawa 216-0001, Japan. gotok@ciea.or.jp
Ishihara
K I
KI
Kuzuoka
A
A
Ohnishi
Y
Y
Itoh
T
T
D000818:Animals; D015925:Cryopreservation; D005243:Feces / Q000821:virology; D016998:Helicobacter / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification; D016481:Helicobacter Infections / Q000382:microbiology* / Q000635:transmission; D006801:Humans; D051379:Mice; D016513:Mice, SCID; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000821:virology*; D016133:Polymerase Chain Reaction; D014183:Transplantation, Heterologous