Involvement of heat shock proteins in the healing of acetic acid-induced gastric ulcers in rats.
The present study examined the expression of 73-kDa of heat shock cognate protein (HSC70), 72-kDa of heat shock protein (HSP70) and 47-kDa of HSP (HSP47) observed in the ulcer healing process in rats. Gastric ulcers were induced by a luminal application of acetic acid in male Donryu rats. During the ulcer healing process, the expression of HSPs in the ulcerated tissue was determined. A high level of HSC70 expression was observed both in the normal mucosa and ulcerated tissue, but the level did not change upon ulceration and ulcer healing. While HSP70 and HSP47 were markedly expressed in the ulcer base during ulceration, and decreased with ulcer healing. HSP70 expression in the ulcer margin was gradually increased with ulcer healing. Omeprazole accelerated the healing of gastric ulcers with strong inhibition of gastric acid secretion, while indomethactin delayed in ulcer healing despite slight inhibition of gastric acid secretion. Omperazole enhanced the expression of HSP70 both in the ulcer margin and base, but it reduced HSP47 expression in the ulcer base Indomethacin markedly enhanced HSP47 expression only in the ulcer base. In conclusion, the expression of HSP70 and HSP47 is changed during ulcer healing. Furthermore, it was suggested that the enhanced expression of HSP70 is involved in acceleration of ulcer healing, but overexpression of HSP47 is involved in delayed ulcer healing.
Tsukimi
Y
Y
Department of Applied Pharmacology, Kyoto Pharmaceutical University, Misasagi, Yamashina, Japan.
Nakai
H
H
Itoh
S
S
Amagase
K
K
Okabe
S
S
D000818:Animals; D050883:HSC70 Heat-Shock Proteins; D050958:HSP47 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins / Q000502:physiology*; D006360:Heat-Shock Proteins / Q000502:physiology*; D007213:Indomethacin / Q000494:pharmacology; D008297:Male; D009853:Omeprazole / Q000494:pharmacology; D051381:Rats; D013276:Stomach Ulcer / Q000188:drug therapy / Q000503:physiopathology*
D050883:HSC70 Heat-Shock Proteins; D050958:HSP47 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins; C494373:Hspa8 protein, rat; C494662:Serpinh1 protein, rat; D009853:Omeprazole; D007213:Indomethacin