title: Tolerable dietary lipid content that does not alter insulin secretion.
abstract: Lipids, either as membrane components or as fuel, are important nutrients that can affect insulin secretion. The aim of this study was to establish the maximum tolerable amount of fat present in the diet, which does not induce significant alteration in the process of insulin secretion. For that, just-weaned male albino rats (70-90 g body weight) were fed during 6 weeks with diets for growing rodents containing 7% fat (A Group) as recommended by the American Institute of Nutrition-AIN. Two other groups in which the fat content of the diet was increased to reach 10% (B Group) or 13% (C Group) were also included. Insulin release, 86Rb+ and 45Ca2+ Fractional Outflow Rate (FOR) during the process of glucose-induced insulin secretion was determined in perfused islets isolated from these animals. No statistical differences in these parameters were detected between A and B rats. However, in the C group, a lower 86Rb+ FOR was found during the whole experiment and a poor insulin secretory response to glucose stimulus was observed. These results led us to postulate that the maximal limiting amount of total lipids present in the diet that does not impair the process of glucose-induced insulin secretion is 10%. These findings authorize future studies on the interference of different dietary lipid sources, in a content 43% more elevated than that recommended (10% against 7%), on the mechanisms of insulin secretion.
authors.affiliation: Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of São Paulo, SP, Brazil.
authors.forename: M C
authors.initials: MC
authors.lastname: Picinato
authors.forename: S M
authors.initials: SM
authors.lastname: Cozzolino
authors.forename: J C
authors.initials: JC
authors.lastname: Gonçalves
authors.forename: M S
authors.initials: MS
authors.lastname: da Rocha
authors.forename: R
authors.initials: R
authors.lastname: Curi
authors.forename: A R
authors.initials: AR
authors.lastname: Carpinelli
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects; D004041:Dietary Fats / Q000008:administration & dosage / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005227:Fatty Acids / Q000008:administration & dosage / Q000494:pharmacology*; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D008297:Male; D051381:Rats
chemical_list: D004041:Dietary Fats; D005227:Fatty Acids; D007328:Insulin