title: Reactivity of dog sera to whole-cell or recombinant antigens of Borrelia burgdorferi by ELISA and immunoblot analysis.
abstract: Enzyme-linked immunosorbent assays (ELISAs) with separate preparations of 10 purified recombinant antigens of Borrelia burgdorferi sensu stricto were used to test sera from 36 dogs not vaccinated with whole cells of this agent and from five dogs vaccinated with whole-cell B. burgdorferi bacteria. All dogs lived in tick-infested areas of Connecticut and south-eastern New York state, USA. The non-vaccinated dogs had limb or joint disorder, lameness and fever during the period 1984-1991 and had antibodies to B. burgdorferi, as determined by a polyvalent ELISA with whole-cell antigen. In re-analyses of sera for total immunoglobulins in ELISAs with recombinant antigens, reactions were most frequently recorded when outer-surface protein (Osp) F, protein (p)35, p37, p39 and p-41G (a flagellin component) were tested separately. Western immunoblots of a subset of 16 sera, positive by ELISA with whole-cell antigen and representing a range of antibody titres (640-40960), verified immune responses to these or other lysed whole-cell antigens. Sera from vaccinated dogs contained antibodies to OspA, OspB, p22, p37 and p41-G. Therefore, serological reactions to OspF, p35 and p39 were the most important indicators of natural exposure to B. burgdorferi. Serum reactivities to these recombinant antigens in ELISAs can be used to help identify possible natural infections of canine borreliosis in dogs not vaccinated with whole-cell B. burgdorferi and to provide information on the geographic distribution of this bacterium.
authors.forename: Louis A
authors.initials: LA
authors.lastname: Magnarelli
authors.affiliation: Department of Entomology, Connecticut Agricultural Experiment Station, New Haven, CT 06504, *Durham Veterinary Hospital, 178 Parmelee Hill Road, Durham, CT, †Section of Rheumatology, Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06520 and ‡Division of Rheumatic Diseases, Department of Medicine, University of Connecticut Health Center, Farmington, CT 06030, USA.
authors.forename: Steven A
authors.initials: SA
authors.lastname: Levy
authors.affiliation: Department of Entomology, Connecticut Agricultural Experiment Station, New Haven, CT 06504, *Durham Veterinary Hospital, 178 Parmelee Hill Road, Durham, CT, †Section of Rheumatology, Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06520 and ‡Division of Rheumatic Diseases, Department of Medicine, University of Connecticut Health Center, Farmington, CT 06030, USA.
authors.forename: Jacob W
authors.initials: JW
authors.lastname: Ijdo
authors.affiliation: Department of Entomology, Connecticut Agricultural Experiment Station, New Haven, CT 06504, *Durham Veterinary Hospital, 178 Parmelee Hill Road, Durham, CT, †Section of Rheumatology, Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06520 and ‡Division of Rheumatic Diseases, Department of Medicine, University of Connecticut Health Center, Farmington, CT 06030, USA.
authors.forename: Caiyun
authors.initials: C
authors.lastname: Wu
authors.affiliation: Department of Entomology, Connecticut Agricultural Experiment Station, New Haven, CT 06504, *Durham Veterinary Hospital, 178 Parmelee Hill Road, Durham, CT, †Section of Rheumatology, Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06520 and ‡Division of Rheumatic Diseases, Department of Medicine, University of Connecticut Health Center, Farmington, CT 06030, USA.
authors.forename: Steven J
authors.initials: SJ
authors.lastname: Padula
authors.affiliation: Department of Entomology, Connecticut Agricultural Experiment Station, New Haven, CT 06504, *Durham Veterinary Hospital, 178 Parmelee Hill Road, Durham, CT, †Section of Rheumatology, Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06520 and ‡Division of Rheumatic Diseases, Department of Medicine, University of Connecticut Health Center, Farmington, CT 06030, USA.
authors.forename: Erol
authors.initials: E
authors.lastname: Fikrig
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology; D000942:Antigens, Bacterial / Q000276:immunology; D015153:Blotting, Western / Q000662:veterinary; D015748:Borrelia burgdorferi Group / Q000254:growth & development / Q000276:immunology*; D003237:Connecticut / Q000453:epidemiology; D004283:Dog Diseases / Q000453:epidemiology / Q000276:immunology* / Q000382:microbiology; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D008193:Lyme Disease / Q000453:epidemiology / Q000276:immunology / Q000662:veterinary*; D022123:Lyme Disease Vaccines / Q000276:immunology / Q000592:standards; D009518:New York / Q000453:epidemiology; D011994:Recombinant Proteins / Q000276:immunology; D016036:Seroepidemiologic Studies; D013984:Tick Infestations; D014611:Vaccination / Q000662:veterinary
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D022123:Lyme Disease Vaccines; D011994:Recombinant Proteins