Separation and determination of podophyllum lignans by micellar electrokinetic chromatography.
A micellar electrokinetic chromatography method was established for the quantitative analysis of seven podophyllum lignans in Podophyllum emodi Wall. var. chinesis sprague. The optimum buffer system was 10 mM NaH2PO4-5 mM borate-100 mM sodium dodecylsulfate-30% isopropanol (pH 7.20). Voltage was 18 kV and detection at 214 nm. The second derivative chromatogram was used to determine a low-content component and those not fully separated from adjacent ones. The RSD values of migration times and peak areas were <2.2 and <5.5%, respectively. The effects of several CE parameters on the resolutions were studied systematically.
Liu
S
S
Department of Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Gansu Province, China.
Tian
X
X
Chen
X
X
Hu
Z
Z
D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D017705:Lignans / Q000032:analysis / Q000302:isolation & purification*; D011035:Podophyllum / Q000737:chemistry*; D015203:Reproducibility of Results; D012967:Sodium Dodecyl Sulfate
D017705:Lignans; D012967:Sodium Dodecyl Sulfate