Structural characterization of beta-cardiac myosin subfragment 1 in solution.
beta-cardiac myosin subfragment 1 (betaS1) tertiary structure and dynamics were characterized with proteolytic digestion, nucleotide analogue trapping kinetics, and intrinsic fluorescence changes accompanying nucleotide binding. Proteolysis of betaS1 produces the 25, 50, and 20 kDa fragments and a new cut within the 50-kDa fragment at Arg369. F-actin inhibits cleavage of the 50-kDa fragment and fails to inhibit cleavage at the 50/20 kDa junction, suggesting betaS1 presents an actoS1 conformation fundamentally different from skeletal S1. Time-dependent changes in Mg(2+)-ATPase accompanying proteolysis identifies cleavage points that lie within the energy transduction pathway. The nucleotide analogue trapping kinetics reveal the presence of a reversible weakly actin attached state. Comparison of nucleotide analogue induced betaS1 structures with the transient structures occurring during ATPase indicates analogue induced and transient structures are in a one-to-one correspondence. Tryptophan fluorescence enhancement accompanies the binding or trapping of nucleotide or nucleotide analogues. Isolation of Trp508 fluorescence shows it is an ATP-sensitive tryptophan and that its vicinity changes conformation sequentially with the transient intermediates accompanying ATPase. These studies elucidate energy transduction and suggest how mutations of betaS1 implicated in disease might undermine function, stability, or efficiency.
Ajtai
K
K
Department of Biochemistry and Molecular Biology, Mayo Foundation, 200 First Street SW, Rochester, Minnesota 55905, USA.
Garamszegi
S P
SP
Park
S
S
Velazquez Dones
A L
AL
Burghardt
T P
TP
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D002942:Circular Dichroism; D005453:Fluorescence; D006868:Hydrolysis; D007700:Kinetics; D008958:Models, Molecular; D018482:Muscle, Skeletal / Q000737:chemistry / Q000201:enzymology; D009206:Myocardium / Q000737:chemistry*; D015879:Myosin Subfragments / Q000737:chemistry* / Q000378:metabolism; D011487:Protein Conformation; D011817:Rabbits; D012996:Solutions
D015879:Myosin Subfragments; D012996:Solutions; D000251:Adenosine Triphosphatases