[Basic study about the development of the hybrid-artificial liver support system using human hepatoma cell lines (Hep G2, Huh 7): effects on liver functions by extracellular matrix (type I collagen) in monolayer culture].
The risk of xenozoonosis infections poses the greatest obstacles against the clinical application of hybrid-artificial liver support system (HALSS). To resolve this issue, we used human hepatoma cell lines (Hep G2, Huh 7) in a type I collagen-coated monolayer culture system, and analyzed liver specific functions such as ammonia removal and albumin synthesis capacity. Ammonia removal activity (nmol/10(6) nuclei/hour) and albumin synthesis activity (microgram/10(6) nuclei/day) were upregulated in both Hep G2 and Huh 7 by type I collagen-coated monolayer culture. In particular, Hep G2 cultured in type I collagen-coated monolayer demonstrated relatively high ammonia removal and albumin synthesis capacity. These results indicate the possibility of the application of human hepatocytes to HALSS.
Fujii
M
M
Faculty of Medicine, Kyushu University, Fukuoka 812-8582.
Yamashita
Y
Y
Shirabe
K
K
Ijima
H
H
Nakazawa
K
K
Funatsu
K
K
Sugimachi
K
K
D000418:Albumins / Q000096:biosynthesis; D000641:Ammonia / Q000378:metabolism; D006528:Carcinoma, Hepatocellular; D024042:Collagen Type I / Q000494:pharmacology*; D005109:Extracellular Matrix; D006801:Humans; D008099:Liver / Q000166:cytology* / Q000502:physiology*; D008113:Liver Neoplasms; D019164:Liver, Artificial; D014407:Tumor Cells, Cultured; D015854:Up-Regulation
D000418:Albumins; D024042:Collagen Type I; D000641:Ammonia