Effect of a vitamin D3-based nutritional supplement ('Videchol') on carbohydrate metabolism of rats following chronic low dose-rate irradiation.
In this study we evaluated the effects of the administration of a vitamin D3 preparation 'Videchol' to chronically irradiated rats (1 cGy day(-1)) by the assessment of the activities of several glycolytic enzymes: lactic dehydrogenase (LDH) (EC 1.1.1.28), pyruvate kinase (PK) (EC 2.7.1.40) and hexokinase (HK) (EC 2.7.1.1), in populations of erythroid and myeloid bone marrow cells. Videchol treatment of irradiated rats led to the normalisation of HK and LDH activity at cumulative doses of around 30 cGy in granulocyte-monocyte cells and to normalisation of LDH and PK activities in erythroid cells starting at 20 cGy in comparison with irradiated rats who did not receive Videchol. The reaction kinetic parameters of LDH in erythrocytes changed according to the redistribution pattern of the isozymes throughout the different stages of the experiment. The administration of Videchol to irradiated rats led to a rearrangement of the LDH isozymes ratio characterised by kinetic properties more comparable to those of the controls. Thus, vitamin D3 appears to induce a normalisation of carbohydrate metabolism in rats chronically irradiated with low dose-rate ionising radiation.
Starikovich
L S
LS
Lviv Ivan Franko National University, Department of Biochemistry, Lviv, Ukraine.
Aragon
G A
GA
Vernikovska
Y I
YI
Vigovska
T V
TV
Veliky
M M
MM
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects / Q000378:metabolism*; D002762:Cholecalciferol / Q000494:pharmacology*; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism*; D006019:Glycolysis / Q000528:radiation effects*; D006593:Hexokinase / Q000378:metabolism; D007527:Isoenzymes / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D011770:Pyruvate Kinase / Q000378:metabolism; D011829:Radiation Dosage; D051381:Rats; D017208:Rats, Wistar; D014916:Whole-Body Irradiation
D007527:Isoenzymes; D002762:Cholecalciferol; D007770:L-Lactate Dehydrogenase; D006593:Hexokinase; D011770:Pyruvate Kinase