Oesophagostomum dentatum: expression patterns of enzymes involved in eicosanoid production.
Cytosolic and membrane-bound proteins of various stages of Oesophagostomum dentatum, the nodular worm of pigs, were investigated for the presence of lipoxygenases (LOX) and cyclooxygenases (COX) using polyclonal and monoclonal antibodies. Putative 12-LOX and 15-LOX, but not 5-LOX, were detected in both fractions of all developmental stages in the expected size range of 75 kDa, with an isoelectric point of 6.0-6.5. The protein could be precipitated with 50% ammonium sulfate, as described for mammalian LOX. An antibody directed against both COX isoforms and one against mammalian COX-2 detected proteins of approximately 70 kDa with an isoelectric point of 6.0-6.5 in the membrane-bound fractions of third-stage larvae and adults, but not in the fourth-stage larvae. Anti-COX-1 or more specific anti-COX-2 antibodies failed to detect proteins. The constitutive LOX expression supports the assumption that the metabolites of this enzyme previously detected in O. dentatum serve intrinsic functions, while the production of anti-inflammatory COX-products in the invasive and luminal stages of the parasite implies a possible role in host-parasite interactions.
Joachim
A
A
Institut für Parasitologie, Tierärztliche Hochschule Hannover, Bünteweg 17, D-30559 Hannover, Germany.
Ruttkowski
B
B
Daugschies
A
A
D000818:Animals; D015153:Blotting, Western; D015777:Eicosanoids / Q000378:metabolism*; D007525:Isoelectric Focusing; D008084:Lipoxygenase / Q000378:metabolism*; D009814:Oesophagostomiasis / Q000469:parasitology / Q000662:veterinary; D009815:Oesophagostomum / Q000201:enzymology* / Q000254:growth & development*; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism*; D013552:Swine; D013553:Swine Diseases / Q000469:parasitology
D015777:Eicosanoids; D008084:Lipoxygenase; D011451:Prostaglandin-Endoperoxide Synthases