Lack of effect of fish oil supplementation on coagulation and transcapillary escape rate of albumin in insulin-dependent diabetic patients with diabetic nephropathy.
OBJECTIVE
We studied the effect of a diet supplementation with fish oil in insulin-dependent diabetic patients with nephropathy in order to evaluate whether abnormal transcapillary escape rate of albumin and procoagulant activity in these patients could be modified.


METHODS
A double-blind, randomized, controlled study was carried out at a tertiary referral centre. The subjects were 29 insulin-dependent diabetic patients with nephropathy. One year of fish oil supplementation (4.6 g n-3 fatty acids/day) was compared with placebo (olive oil). The main outcome measures were N-3 fatty acid proportions of platelet lipids, transcapillary escape rate of albumin, prothrombin fragment 1 + 2, thrombin-antithrombin complexes, markers of fibrinolysis, fibrinogen, factor VII antigen and activity, thrombomodulin, von Willebrand factor, platelet factor 4 and beta-thromboglobulin. These were measured every 6 months.


RESULTS
Neither transcapillary escape rate of albumin (7.4 (median) (5.0-9.8) (range) % vs. 7.0 (4.6-10.6) %) nor prothrombin fragment 1 + 2 (0.97 (0.72-2.40) nmol/L vs. 1.01 (0.59-3.11) nmol/L) changed after 12 months of fish oil supplementation.


CONCLUSIONS
Increased transcapillary escape rate of albumin and activity could not be modified during diet supplementation with fish oil in insulin-dependent diabetic patients with nephropathy.
Myrup
B
B
Steno Diabetes Center, Gentofte, Denmark.
Rossing
P
P
Jensen
T
T
Parving
H H
HH
Hølmer
G
G
Gram
J
J
Kluft
C
C
Jespersen
J
J
D000990:Antithrombin III / Q000032:analysis; D001777:Blood Coagulation; D001792:Blood Platelets / Q000737:chemistry; D002196:Capillaries / Q000378:metabolism*; D003922:Diabetes Mellitus, Type 1 / Q000503:physiopathology*; D003928:Diabetic Nephropathies / Q000503:physiopathology*; D004311:Double-Blind Method; D005167:Factor VII / Q000032:analysis / Q000378:metabolism; D015525:Fatty Acids, Omega-3 / Q000097:blood; D005340:Fibrinogen / Q000032:analysis; D005342:Fibrinolysis; D005395:Fish Oils / Q000008:administration & dosage*; D006442:Glycated Hemoglobin / Q000032:analysis; D006801:Humans; D007457:Iodine Radioisotopes; D008055:Lipids / Q000097:blood; D000069463:Olive Oil; D010446:Peptide Fragments / Q000032:analysis; D010447:Peptide Hydrolases / Q000032:analysis; D010919:Placebos; D010938:Plant Oils / Q000008:administration & dosage; D010978:Platelet Factor 4 / Q000032:analysis; D011516:Prothrombin / Q000032:analysis; D012709:Serum Albumin / Q000378:metabolism*; D012711:Serum Albumin, Radio-Iodinated; D018180:Thrombomodulin / Q000097:blood; D001620:beta-Thromboglobulin / Q000032:analysis; D014841:von Willebrand Factor / Q000032:analysis
D000990:Antithrombin III; D005167:Factor VII; D015525:Fatty Acids, Omega-3; D005340:Fibrinogen; D005395:Fish Oils; D006442:Glycated Hemoglobin; D007457:Iodine Radioisotopes; D008055:Lipids; D000069463:Olive Oil; D010446:Peptide Fragments; D010447:Peptide Hydrolases; D010919:Placebos; D010938:Plant Oils; D010978:Platelet Factor 4; D011516:Prothrombin; D012709:Serum Albumin; D012711:Serum Albumin, Radio-Iodinated; D018180:Thrombomodulin; D001620:beta-Thromboglobulin; D014841:von Willebrand Factor; C046193:antithrombin III-protease complex; C045879:prothrombin fragment 1.2