Cannabinoids inhibit nitric oxide production in bone marrow derived feline macrophages.
Feline immunodeficiency virus (FIV) infection causes a widespread natural immunodeficiency syndrome in cats that is considered a suitable animal model for studying human immunodeficiency virus (HIV) infection and pathogenesis. Short term cultures of bone marrow derived feline macrophages stimulated with recombinant feline interferon-gamma (r-IFN-gamma) and lipopolysaccharide (LPS) were shown to produce nitric oxide. Feline macrophages were shown to express cannabinoid receptors, and nitric oxide production decreased after in vitro exposure to synthetic cannabinoid CP-55940. Both cannabinoid receptors, CB1 and CB2, were involved in this process, since the inhibition was reversed by selective cannabinoid antagonists for both of these receptors.
Ponti
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W
Department of Animal Pathology, Hygiene and Veterinary Public Health, Microbiology and Immunology Unit, via Celoria 10, 20133 Milano, Italy. wilma.ponti@unimi.it
Rubino
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T
Bardotti
M
M
Poli
G
G
Parolaro
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D
D000818:Animals; D001854:Bone Marrow Cells / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D001892:Camphanes / Q000494:pharmacology; D002186:Cannabinoids / Q000276:immunology / Q000378:metabolism / Q000494:pharmacology*; D002415:Cats; D003511:Cyclohexanols / Q000494:pharmacology; D004195:Disease Models, Animal; D016181:Feline Acquired Immunodeficiency Syndrome / Q000276:immunology* / Q000473:pathology; D006651:Histocytochemistry; D007166:Immunosuppressive Agents / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D009569:Nitric Oxide / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D010587:Phagocytosis; D010880:Piperidines / Q000494:pharmacology; D011720:Pyrazoles / Q000494:pharmacology; D043882:Receptors, Cannabinoid; D011955:Receptors, Drug / Q000037:antagonists & inhibitors / Q000378:metabolism; D000077285:Rimonabant
D001892:Camphanes; D002186:Cannabinoids; D003511:Cyclohexanols; D007166:Immunosuppressive Agents; D010880:Piperidines; D011720:Pyrazoles; D043882:Receptors, Cannabinoid; D011955:Receptors, Drug; C110630:SR 144528; D009569:Nitric Oxide; C054649:3-(2-hydroxy-4-(1,1-dimethylheptyl)phenyl)-4-(3-hydroxypropyl)cyclohexanol; D000077285:Rimonabant