Transcription-induced cytosine-to-thymine mutations are not dependent on sequence context of the target cytosine.
We showed previously that transcription of a plasmid-borne kan allele increases C-to-T mutations in the nontranscribed strand. Using two new plasmid-borne kan alleles, one cmp allele, and a chromosomal kan allele, we found in this study that transcription-induced mutations are not limited to specific genes, alleles, or locations and are likely to be a general property of transcript elongation in Escherichia coli.
Beletskii
A
A
Department of Chemistry, Wayne State University, Detroit, Michigan 48202, USA.
Bhagwat
A S
AS
D000483:Alleles; D002875:Chromosomes; D003596:Cytosine; D004269:DNA, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D007613:Kanamycin Resistance / Q000235:genetics; D010957:Plasmids; D017354:Point Mutation; D013941:Thymine; D014158:Transcription, Genetic
D004269:DNA, Bacterial; D003596:Cytosine; D013941:Thymine