Enzyme-linked immunoelectrotransfer blotting analysis of human serologic responses to infective hookworm larval antigen.
OBJECTIVE
To explore the possibility of using specific antigens for immunodiagnosis of hookworm disease in endemic area.


METHODS
Infective third-stage larvae of the canine hookworm, Ancylostoma caninum (A. caninum), were prepared as the source of antigen. Enzyme-linked immunoelectrotransfer blotting (EITB) was employed as an immunodiagnostic method.


RESULTS
Two immunodominant bands of hookworm antigens (42 kDa and 55 kDa) were recognized by the sera of hookworm-infected patients (serum dilution 1:200; antigen centrifuged at 36,000 r/m for 20 minutes, but not by sera from negative controls.


CONCLUSIONS
The 42 kDa and 55 kDa A. caninum antigens might be the specific antigens that could be used for immunodiagnosis of hookworm disease in endemic area.
Xue
H
H
Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine, Shanghai 200025, China.
Liu
S
S
Ren
H
H
Qiang
H
H
Xiao
S
S
Feng
Z
Z
Hotez
P
P
D000722:Ancylostoma / Q000276:immunology*; D000724:Ancylostomiasis / Q000097:blood / Q000175:diagnosis*; D000818:Animals; D000947:Antigens, Helminth / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D007814:Larva / Q000276:immunology
D000947:Antigens, Helminth