title: [Water-soluble ginsenosides in American ginseng].
abstract: OBJECTIVE
  To have further comprehension of American Ginseng on the basis of its water-soluble components.
  
  
  METHODS
  The water suspension of 80% methanol extract of American Ginseng was sequentially extracted with ethyl ether and n-butyl alcohol. The saccharides in the water-soluble portion were removed by macro-reticular resin column, and three compounds were obtained by silica-gel column chromatography. Their structures were confirmed on the basis of spectral analysis(IR, NMR, MALDI-MS) melting points and optical degrees.
  
  
  RESULTS
  Three compounds were identified as malonyl ginsenosides Rb1, ginsenoside Rb1 and Re respectively. Among them Rb1 and Re are known, while malonyl ginsenoside Rb1 was isolated from American Ginseng for the first time. Its structure was elucidated as (3 beta, 12 beta)-20-[(-6-O-beta-D- glucopyranosyl-beta-D-glucopyranosyl)oxy]-12-hydroxylammar-24-en-3-yl-O- [6-O-(carboxyacetyl)-beta-D-glucopyranosyl)]-beta-D-glucopyranoside.
  
  
  CONCLUSIONS
  Water-soluble ginsenosides were isolated from the water-soluble extract of American Ginseng suggesting that malonyl ginsenosides exist both in American Ginseng and Ginseng.
authors.affiliation: Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022.
authors.forename: Y
authors.initials: Y
authors.lastname: Zhou
authors.forename: F
authors.initials: F
authors.lastname: Song
authors.forename: S
authors.initials: S
authors.lastname: Liu
authors.forename: X
authors.initials: X
authors.lastname: Li
mesh_terms: D036145:Ginsenosides; D005894:Panax / Q000737:chemistry*; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D036145:Ginsenosides; D012503:Saponins; C049864:ginsenoside Re; C089027:ginsenoside Rb1, malonyl