Sequence analysis of hepatitis B virus genomes from an infant with acute severe hepatitis and a hepatitis B e antigen-positive carrier mother.
It is well known that fulminant hepatitis B can occur in infants born to hepatitis B e antigen (HBeAg)-negative hepatitis B virus (HBV) carrier mothers, whereas fulminant hepatitis and severe hepatitis are uncommon in infants born to HBeAg-positive mothers. We have encountered an infant with severe acute hepatitis B born to a HBeAg-positive mother. The aim of this study was to determine whether HBV variants contribute to the pathogenesis of fulminant hepatitis and severe hepatitis in an infant born to an HBeAg-positive mother. The nucleotide sequence of HBV genomes from the infant and his HBeAg-positive carrier mother was analyzed. All HBV isolated from the infant and his mother were subtype adr. The sequences of the cloned HBV genomes, each including a part of the X and precore/core regions, isolated from the infant were almost identical (homology of 99.1-99.9%) to those from his mother. There was no mutation in any of the 17 clones examined at nucleotides 1762 and 1764 in the core promoter, which is reported to be associated with fulminant hepatitis. A point mutation at nucleotide 1758 in the second AT-rich region of the basic core promoter was present in all clones. None of the clones had a point mutation at nucleotide 1896 of the precore region. In this study, no specific HBV variants contributing to the development of neonatal severe hepatitis were found. There is a possibility that host factors rather than viral factors play an important role in some cases of severe neonatal hepatitis B.
Komatsu
H
H
Department of Pediatrics, National Defense Medical College, 3-2, Namiki, Tokorozawa, Saitama 359-8513, Japan. grd1303@gr.ndmc.ac.jp
Inui
A
A
Morinishi
Y
Y
Sogo
T
T
Fujisawa
T
T
D000328:Adult; D001483:Base Sequence; D002353:Carrier State / Q000821:virology*; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D016679:Genome, Viral; D006509:Hepatitis B / Q000635:transmission / Q000821:virology*; D006511:Hepatitis B Antigens / Q000235:genetics; D006513:Hepatitis B e Antigens / Q000097:blood*; D006515:Hepatitis B virus / Q000145:classification / Q000235:genetics*; D006801:Humans; D007231:Infant, Newborn; D018445:Infectious Disease Transmission, Vertical; D008297:Male; D008969:Molecular Sequence Data; D017354:Point Mutation; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000821:virology; D017422:Sequence Analysis, DNA
D004279:DNA, Viral; D006511:Hepatitis B Antigens; D006513:Hepatitis B e Antigens