Removal of PCR inhibitors by silica membranes: evaluating the Amplicor Mycobacterium tuberculosis kit.
The effectiveness of PCR inhibitor removal by silica membranes in combination with the Amplicor Mycobacterium tuberculosis kit was analyzed for 655 respiratory and nonrespiratory specimens. The overall inhibition rate was reduced from 12.5%, when applying the Amplicor kit alone, to 1.1% with the addition of silica membrane DNA purification.
Böddinghaus
B
B
Institute of Medical Microbiology, University Hospital, Frankfurt am Main, Germany. Boeddinghaus@em.uni-frankfurt.de
Wichelhaus
T A
TA
Brade
V
V
Bittner
T
T
D003470:Culture Media; D004269:DNA, Bacterial / Q000302:isolation & purification*; D005374:Filtration / Q000379:methods; D006801:Humans; D008567:Membranes, Artificial; D009169:Mycobacterium tuberculosis / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D012822:Silicon Dioxide; D014376:Tuberculosis / Q000382:microbiology
D003470:Culture Media; D004269:DNA, Bacterial; D008567:Membranes, Artificial; D011933:Reagent Kits, Diagnostic; D012822:Silicon Dioxide