TMC-205 a new transcriptional up-regulator of SV40 promoter produced by an unidentified fungus. Fermentation, isolation, physico-chemical properties, structural determination and biological activities.
A new transcriptional up-regulator designated TMC-205 was discovered from the fermentation broth of an unidentified fungal strain TC 1630 by using an SV40 promoter-luciferase reporter assay. Based on spectroscopic analyses, its structure was determined to be (E)-6-(3-methyl-1,3-butadienyl)- H-indole-3-carboxylic acid. Expression of the luciferase activity was activated ca. 2-, 4-, and 6-fold by 1, 10, and 100 microM TMC-205, respectively. TMC-205 activated the transcriptional activity in a manner dependent on the presence of the enhancer element of SV40 in its promoter region.
Sakurai
M
M
Discovery Research Laboratory, Tanabe Seiyaku Co., Ltd, Saitama, Japan. sakura@tanabe.co.jp
Kohno
J
J
Nishio
M
M
Yamamoto
K
K
Okuda
T
T
Kawano
K
K
Nakanishi
N
N
D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002851:Chromatography, High Pressure Liquid; D004742:Enhancer Elements, Genetic; D005285:Fermentation; D005658:Fungi / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D007211:Indoles / Q000097:blood* / Q000737:chemistry / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D011401:Promoter Regions, Genetic; D013539:Simian virus 40 / Q000235:genetics*; D013057:Spectrum Analysis; D014157:Transcription Factors / Q000096:biosynthesis* / Q000737:chemistry* / Q000494:pharmacology; D014158:Transcription, Genetic / Q000187:drug effects; D014162:Transfection
D007211:Indoles; C437365:TMC-205; D014157:Transcription Factors; C030374:indole