Prediction of the shelf life of cellulose acetate hemodialyzers by Monte Carlo simulation.
A previous investigation by our laboratory linked cellulose acetate degradation with adverse health effects in hemodialysis patients. To establish the accumulation of degradation products with time, a Monte Carlo model of degradation kinetics was developed. The model tracks changes in a population of molecules representative of the dialyzer membrane during the degradation process. The degradation calculation is a two step process: First, the model uses a random number to select an individual polymer molecule out of the population, and then a second random number is used to identify a site on the selected molecule for the degradation reaction to occur. After the reaction calculation, the resulting degraded molecules are redistributed into the population. The course of the reaction is determined by recalculating the molecular weight averages in the changing population as the calculations proceed. The model was validated using gel permeation chromatography molecular weight results and total acetyl content measurements on dialyzers stored up to 13.3 years after manufacture. It was found that the degradation reactions can be accurately modeled as random events and that the chain scissions and deacetylation events occur at constant rates. The shelf life of these devices was estimated using the model predictions and animal test results.
Hutter
J C
JC
Center for Devices and Radiological Health, Food and Drug Administration, Rockville, Maryland 20852, USA.
Long
M C
MC
Luu
H M
HM
Schroeder
L W
LW
D002482:Cellulose / Q000009:adverse effects / Q000031:analogs & derivatives* / Q000737:chemistry; D004355:Drug Stability; D006801:Humans; D066298:In Vitro Techniques; D007688:Kidneys, Artificial / Q000009:adverse effects; D008567:Membranes, Artificial; D008956:Models, Chemical; D008970:Molecular Weight; D009010:Monte Carlo Method; D012449:Safety; D013997:Time Factors
D008567:Membranes, Artificial; C005062:acetylcellulose; D002482:Cellulose