title: Analysis of monocyte chemotactic protein-1 production in different major histocompatability complex-restricted antigen presentation systems.
abstract: In the present study the production of the CC chemokine monocyte chemotactic protein-1 (MCP-1) in several MHC II-restricted antigen presentation systems was investigated in vitro. To assess which type of antigen-presenting cell (APC) influences MCP-1 production during antigen presentation, cultures enriched for different APC populations were prepared and MCP-1 production was determined. Our results showed that APCs that effectively induce a T cell response also produce elevated amounts of MCP-1. The MCP-1 production is highest in the memory-driven secondary response against a single antigen. Despite a massive T cell proliferation, low MCP-1 concentrations are found in Con A-induced cultures. These results suggest that T cell proliferation alone is not sufficient for MCP-1 production and that stimulation of the APC during the process of antigen presentation results in MCP-1 production. Based on our results and the literature, we propose a model for MCP-1 as an enhancer of the adaptive immune response.
authors.affiliation: Department of Cell Biology and Immunology, Faculty of Medicine, Vrije Universiteit, van der Boechorststraat 7, Amsterdam, 1081 BT, The Netherlands.
authors.forename: J
authors.initials: J
authors.lastname: Tekstra
authors.forename: E P
authors.initials: EP
authors.lastname: Tjin
authors.forename: C W
authors.initials: CW
authors.lastname: Tuk
authors.forename: D
authors.initials: D
authors.lastname: Broekhuis-Fluitsma
authors.forename: C E
authors.initials: CE
authors.lastname: Havenith
authors.forename: R H
authors.initials: RH
authors.lastname: Beelen
mesh_terms: D017951:Antigen Presentation; D000938:Antigen-Presenting Cells / Q000276:immunology* / Q000648:ultrastructure; D002478:Cells, Cultured; D018932:Chemokine CCL2 / Q000096:biosynthesis*; D003208:Concanavalin A / Q000494:pharmacology; D000949:Histocompatibility Antigens Class II / Q000502:physiology*; D006801:Humans; D007156:Immunologic Memory; D007519:Isoantigens / Q000276:immunology; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D008264:Macrophages / Q000276:immunology / Q000648:ultrastructure; D013601:T-Lymphocytes / Q000276:immunology / Q000648:ultrastructure; D014019:Tissue Donors
chemical_list: D018932:Chemokine CCL2; D000949:Histocompatibility Antigens Class II; D007519:Isoantigens; D003208:Concanavalin A