Experimental allergic myositis in SJL/J mouse. Reappraisal of immune reaction based on changes after single immunization.
SJL/J mice have been subjected to immunization with wide varieties of antigens to produce models of autoimmune disorders including experimental myositis. They also have a defect in dysferlin gene and spontaneously develop muscle fiber degeneration, a condition akin to limb-girdle type muscular dystrophy and Miyoshi myopathy. To know whether muscle inflammation of SJL mice after immunization with muscle fractions really represents immune-mediated myositis or no more than an epiphenomenon of muscle degeneration due to dysferlin defect, we studied immunological parameters after immunization with rabbit myosin B fraction. Initial infiltration of macrophages and CD4+ lymphocytes on day 11 was followed by increase in number of CD8+ cells. Such increase was not observed in the nontreated and adjuvant controls. Some infiltrating cells were interferon gamma (IFN-gamma) positive. Furthermore, increased expression of the signal transducers and activator of transcription 1 (STAT-1) and interferon regulatory factor 1 (IRF-1) mRNA was shown in the first 2 weeks. These results indicate Th1 system activity in the muscle, rather than simple dysferlin deficiency, particularly 1-3 weeks after immunization. Thus it is concluded that an immune-mediated myositis is taking place at this stage. This model can be helpful in understanding pathomechanisms involved in the early stage of human myositides. It has also important implications concerning immune reactions associated with transplantation or gene therapy for muscular dystrophies.
Matsubara
S
S
Department of Neurology, Tokyo Metropolitan Neurological Hospital, 2-6-1 Musashidai, Fuchu, 1830042, Tokyo, Japan. mtbrs@tmnh.fuchu.tokyo.jp
Kitaguchi
T
T
Kawata
A
A
Miyamoto
K
K
Yagi
H
H
Hirai
S
S
D000818:Animals; D017931:DNA Primers; D004268:DNA-Binding Proteins / Q000032:analysis / Q000235:genetics; D000073939:Dysferlin; D005260:Female; D015870:Gene Expression / Q000276:immunology; D007114:Immunization; D007150:Immunohistochemistry; D050856:Interferon Regulatory Factor-1; D007371:Interferon-gamma / Q000032:analysis / Q000235:genetics; D008565:Membrane Proteins; D051379:Mice; D008815:Mice, Inbred Strains; D009124:Muscle Proteins / Q000235:genetics; D018482:Muscle, Skeletal / Q000737:chemistry / Q000276:immunology* / Q000473:pathology*; D009137:Muscular Dystrophy, Animal / Q000276:immunology; D020721:Nervous System Autoimmune Disease, Experimental / Q000276:immunology* / Q000473:pathology*; D010750:Phosphoproteins / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D050794:STAT1 Transcription Factor; D015534:Trans-Activators / Q000032:analysis / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis / Q000235:genetics
D017931:DNA Primers; D004268:DNA-Binding Proteins; C114839:DYSF protein, human; D000073939:Dysferlin; D050856:Interferon Regulatory Factor-1; C494258:Irf1 protein, mouse; D008565:Membrane Proteins; D009124:Muscle Proteins; D010750:Phosphoproteins; D012333:RNA, Messenger; D050794:STAT1 Transcription Factor; C494080:Stat1 protein, mouse; D015534:Trans-Activators; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma