title: Genome-wide chromatin remodeling modulates the Alu heat shock response.
abstract: During heat shock recovery in Hela cells, the level of Alu RNA transiently increases with kinetics that approximately parallel the transient expression of heat shock protein mRNAs. Coincidentally, there is a transient increase in the accessibility of Alu chromatin to restriction enzyme cleavage suggesting that an opening and re-closing of chromatin regulates the Alu stress response. Similar changes occur in alpha satellite and LINE1 chromatin showing that heat shock induces a genome-wide remodeling of chromatin structure which is independent of transcription. The increased accessibility of restriction sites within these repetitive sequences is inconsistent with a simple lengthening of the nucleosome linker region but instead suggests a scrambling of nucleosome positions. Chromatin structure and its dynamics account for many of the principal features of SINE transcriptional regulation potentially providing a functional rationale for the dispersion and high copy number of SINEs.
authors.affiliation: Department of Chemistry, University of California, Davis, CA 95616-8535, USA.
authors.forename: C
authors.initials: C
authors.lastname: Kim
authors.forename: C M
authors.initials: CM
authors.lastname: Rubin
authors.forename: C W
authors.initials: CW
authors.lastname: Schmid
mesh_terms: D020087:Alu Elements / Q000235:genetics*; D002843:Chromatin / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D015894:Genome, Human; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D006367:HeLa Cells; D018869:Heat-Shock Response / Q000235:genetics*; D006358:Hot Temperature; D006801:Humans; D020084:Long Interspersed Nucleotide Elements / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012334:RNA, Neoplasm / Q000235:genetics / Q000378:metabolism
chemical_list: D002843:Chromatin; D018840:HSP70 Heat-Shock Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm