title: Differential properties of mucous glycoproteins in rat nasal epithelium. A comparison between allergic inflammation and lipopolysaccharide stimulation.
abstract: To examine the differential properties of mucous glycoproteins, we produced hypertrophic and metaplastic changes in goblet cells of rat nasal epithelium by intranasal instillation of ovalbumin (OVA) in OVA-sensitized rats, and by intranasal lipopolysaccharide (LPS) instillation. The epithelial mucosubstance was quantitatively examined by alcian blue-periodic acid-Schiff (AB-PAS) and lectin histochemistry. The newly produced mucin after OVA challenge or LPS instillation contained a high amount of sulfomucin and a low amount of neutral glycoprotein: LPS-induced mucin contained more sulfomucin (70.1% of total) and less neutral glycoprotein (8.6%) than OVA-induced mucin (sulfomucin, 33.6%; neutral glycoprotein, 41.8%; p < 0.01). Four of the lectins stained some of the mucosubstance, indicating the presence of galactose-N-acetylgalactosamine, alpha2,3- and alpha2,6-linked sialic acid-galactose, and fucose residues. After LPS instillation, the reactivity was higher for galactose-N-acetylgalactosamine (64.8% of total) and alpha2,3-linked sialic acid-galactose (75.8%) than after saline instillation (3.5 and 19.1%, respectively) or OVA challenge (5.8 and 32.3%; p < 0.05). OVA challenge did not induce the alteration of terminal sugar residues. A 2-fold increase in mucin mRNA (rat Muc5ac) expression was induced after LPS instillation or OVA challenge, compared with animals treated with saline instillation (p < 0.05). These results indicate that mucin mRNA expression (for peptide backbone) increases similarly after LPS instillation or OVA challenge; however, carbohydrate compositions of newly produced mucin are different between the two groups.
authors.affiliation: Department of Otorhinolaryngology, Mie University School of Medicine, Mie 514, Japan. tshimizu@clin.medic.mie-u.ac.jp
authors.forename: T
authors.initials: T
authors.lastname: Shimizu
authors.forename: H
authors.initials: H
authors.lastname: Hirano
authors.forename: S
authors.initials: S
authors.lastname: Shimizu
authors.forename: C
authors.initials: C
authors.lastname: Kishioka
authors.forename: Y
authors.initials: Y
authors.lastname: Sakakura
authors.forename: Y
authors.initials: Y
authors.lastname: Majima
mesh_terms: D000818:Animals; D003627:Data Interpretation, Statistical; D004848:Epithelium / Q000378:metabolism / Q000473:pathology / Q000502:physiology; D020397:Goblet Cells / Q000378:metabolism / Q000473:pathology / Q000502:physiology*; D006651:Histocytochemistry; D006967:Hypersensitivity / Q000473:pathology / Q000503:physiopathology*; D007249:Inflammation / Q000473:pathology / Q000503:physiopathology*; D037102:Lectins; D008070:Lipopolysaccharides / Q000008:administration & dosage*; D008297:Male; D009077:Mucins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D009093:Mucus / Q000378:metabolism / Q000502:physiology; D010047:Ovalbumin / Q000008:administration & dosage; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D011916:Rats, Inbred F344; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012965:Sodium Chloride / Q000008:administration & dosage
chemical_list: D037102:Lectins; D008070:Lipopolysaccharides; D009077:Mucins; D012333:RNA, Messenger; D012965:Sodium Chloride; D010047:Ovalbumin