[Inhibiting effect of antisense oligodeoxynucleotide of alpha 1(I) Precollagen gene on hypertrophic scar in an animal model].
OBJECTIVE
To study the effect of antisense oligodeoxynucleotide (ODN) of alpha 1(I) precollagen gene on hypertrophic scar in an animal model.


METHODS
Hypertrophic scar was explanted in athymic mice. The effect of antisense oligodeoxynucleotide 1 and 2 on the animal model and the inhibition of ODN1 and ODN2 were observed.


RESULTS
It was showed that antisense oligo (ODN1, 21 bp) located 5'end from the translation start region and antisense oligo (ODN2, 22 bp) between the first exon and the first intron could effectively inhibit scar hypertrophy. However the control groups showed no inhibition.


CONCLUSIONS
Antisense oligodeoxynucleotide could effectively inhibit scar hypertrophy by inhibiting the synthesis of type I collagen protein.
Qi
S
S
Department of Burn Surgery, First Affiliated Hospital, Sun Yat-sen University of Medical Science, Guangzhou 510080, China.
Li
T
T
Shan
Y
Y
D000818:Animals; D002056:Burns / Q000150:complications; D017439:Cicatrix, Hypertrophic / Q000235:genetics / Q000378:metabolism / Q000628:therapy*; D003094:Collagen / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D015316:Genetic Therapy; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D020319:Oligodeoxyribonucleotides, Antisense / Q000627:therapeutic use*
D020319:Oligodeoxyribonucleotides, Antisense; D003094:Collagen