Evaluation of BacT/Alert 3D liquid culture system for recovery of mycobacteria from clinical specimens using sodium dodecyl (lauryl) sulfate-NaOH decontamination.
A total of 52 mycobacterial isolates were recovered from 1,197 clinical specimens decontaminated by a sodium dodecyl (lauryl) sulfate (SDS)-NaOH protocol. Of these, 94% were recovered with the BacT/Alert 3D system (Organon Teknika, Durham, N.C.) and 79% were recovered on Löwenstein-Jensen (LJ) medium. Mean times to detection of organisms of the Mycobacterium tuberculosis complex (n = 47) were 22.8 days with LJ medium and 16.2 days with the system. The BacT/Alert 3D system is a rapid and efficient detection system which can be used with an SDS-NaOH decontamination procedure.
Carricajo
A
A
Department of Bacteriology, CHU Bellevue Hospital, Saint-Etienne, France. anne.carricajo@uni-st-etienne.fr
Fonsale
N
N
Vautrin
A C
AC
Aubert
G
G
D001431:Bacteriological Techniques; D003470:Culture Media; D004203:Disinfection / Q000379:methods; D006801:Humans; D009161:Mycobacterium / Q000254:growth & development / Q000302:isolation & purification*; D009164:Mycobacterium Infections / Q000382:microbiology*; D009169:Mycobacterium tuberculosis / Q000254:growth & development / Q000302:isolation & purification; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D012972:Sodium Hydroxide / Q000494:pharmacology; D014376:Tuberculosis / Q000382:microbiology
D003470:Culture Media; D012967:Sodium Dodecyl Sulfate; D012972:Sodium Hydroxide