[Effect of argon and nitrogen on the peritoneal macrophages in mice and their resistance to the UV damaging effect in vitro].
Explored were effects of argon and nitrogen on intracellular pH in peritoneal macrophages in mice and resistance of cellular membranes to the UV damaging effect in vitro. Blasting argon or nitrogen along the surface of cell cultures in airtight chamber for 20 minutes was shown to decrease 5-folds the oxygen content of solution as compared with initial level with culture pH unchanged. Ten-minute blasting argon or nitrogen through the incubation chamber slightly elevates intracellular pH in macrophages. The standard cell incubation conditions recovered following approximately 60 minutes in hypoxic atmosphere, the ability of macrophages to build up fluorescein was degraded and they increased intracellular pH no matter the indifferent gas yet more marked in case of nitrogen in use. It was demonstrated that the normobaric gas environment with oxygen partly replaced by nitrogen or argon protects plasmatic membranes of cells from UV-induced damage.
Galchuk
S V
SV
Turovetskiĭ
V B
VB
Andreev
A I
AI
Buravkova
L B
LB
D000818:Animals; D001128:Argon / Q000494:pharmacology*; D001273:Atmosphere Exposure Chambers; D002462:Cell Membrane / Q000378:metabolism / Q000528:radiation effects; D006863:Hydrogen-Ion Concentration; D007113:Immunity, Innate; D066298:In Vitro Techniques; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D051379:Mice; D009584:Nitrogen / Q000494:pharmacology*; D011827:Radiation
D001128:Argon; D009584:Nitrogen