Affinity driven molecular transfer from erythrocyte membrane to target cells.
A wide variety of antimicrobial peptides are known to bind to - and disrupt microbial plasma membranes. Recently, derivatives of the antimicrobial peptide dermaseptin S4 were shown to selectively disrupt the plasma membrane of the intracellular parasite Plasmodium falciparum without harming that of the mammalian host cell. The resulting antimalarial activity is allegedly exerted after the harmless peptide binding to the membrane of the host cell, followed by peptide translocation across a number of intracellular membrane systems and interaction with that of the intraerythrocyte parasite. In this study, we present evidence in support of the ability of a membrane-bound peptide, the dermaseptin S4 derivative K(4)-S4(1-13)a, to transfer from red blood cells (RBCs) to another distant membrane. Binding of K(4)-S4(1-13)a to the plasma membrane of RBCs was assessed in vitro and in vivo, and found to be rapid, spontaneous and receptor independent, as was the transfer of the RBC-bound peptide to the plasma membrane of microorganisms. The present study further provides a basis for the possible use of RBCs as a transport vehicle to deliver drugs to distant targets. This drug delivery system involves the transient "loading" of RBCs with a lipophilic "hook" peptide. Such a peptide has enough affinity for the RBC's plasma membrane to bind to the membrane, but given the opportunity, the peptide will exit its position and transfer to another (target) cell for which it has a greater affinity. The efficacy of such an affinity driven transfer system was demonstrated experimentally by the transfer of K(4)-S4(1-13)a from pre-loaded RBCs to bacteria, yeast and protozoan target cells.
Feder
R
R
The Laboratory for Antimicrobial Peptides Investigation (L.A.P.I.), The Hebrew University of Jerusalem, Givat Ram 91904, Jerusalem, Israel.
Nehushtai
R
R
Mor
A
A
D029845:Amphibian Proteins; D000818:Animals; D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents / Q000378:metabolism; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry / Q000378:metabolism*; D001419:Bacteria / Q000166:cytology / Q000378:metabolism*; D001667:Binding, Competitive / Q000502:physiology*; D001692:Biological Transport / Q000502:physiology; D002450:Cell Communication / Q000502:physiology; D002462:Cell Membrane / Q000378:metabolism*; D016503:Drug Delivery Systems; D004912:Erythrocytes / Q000166:cytology / Q000378:metabolism*; D056890:Eukaryota / Q000166:cytology / Q000378:metabolism; D006790:Host-Parasite Interactions / Q000502:physiology; D009842:Oligopeptides / Q000737:chemistry / Q000378:metabolism*; D015003:Yeasts / Q000166:cytology / Q000378:metabolism
D029845:Amphibian Proteins; D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D023181:Antimicrobial Cationic Peptides; C441195:K4-S4(1-13)a; D009842:Oligopeptides; C109653:dermaseptin 4 protein, Phyllomedusa