title: Enhanced antifungal activity of ketoconazole by Euphorbia characias latex against Candida albicans.
abstract: The in vitro suseptibility of Candida albicans to ketoconazole and Euphorbia characias latex alone or in combination was tested using the macrobroth dilution method. The MIC 80% of crude latex and ketoconazole are respectively 159 microg protein/ml and 0.3901 microg/ml. This method permits us to determine an affinity constant K(aff) for crude latex (0.015 microg(-1) protein ml) and ketoconazole (23.828 microg(-1) ml). The utilization of a mixture of latex at several concentrations (7.8-15.62-31.25-62.5 and 125 microg protein/ml) and ketoconazole indicates a synergistic effect between latex and ketoconazole. For latex concentrations of 31.25 and 62.5 microg protein/ml the MIC 80% of ketoconazole were inferior (0.194 and 0.183 microg/ml respectively) to that obtained with ketoconazole alone (0.390 microg/ml). A synergistic effect is therefore obtained between ketoconazole on the one hand and two concentrations of Euphorbia characias latex.
authors.affiliation: Université de la Méditerranée, Faculté de Pharmacie, 27 Bd Jean Moulin, 13385 Cedex 05, Marseille, France.
authors.forename: R
authors.initials: R
authors.lastname: Giordani
authors.forename: J
authors.initials: J
authors.lastname: Trebaux
authors.forename: M
authors.initials: M
authors.lastname: Masi
authors.forename: P
authors.initials: P
authors.lastname: Regli
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D002176:Candida albicans / Q000187:drug effects*; D003470:Culture Media; D004357:Drug Synergism; D028481:Euphorbia / Q000737:chemistry*; D005656:Fungal Proteins / Q000378:metabolism; D006027:Glycosides / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007654:Ketoconazole / Q000494:pharmacology*; D007840:Latex / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D010946:Plants, Medicinal / Q000737:chemistry*
chemical_list: D000935:Antifungal Agents; D003470:Culture Media; D005656:Fungal Proteins; D006027:Glycosides; D007840:Latex; D007654:Ketoconazole