Cloning and characterization of a novel transcription factor involved in cellular proliferation arrest: PATF.
Cell cycle withdrawal involves several transcription factors such as E2Fs members that play a key role in cell growth control. Here we describe a novel putative bZIP transcription factor isolated from the retina and involved in neuronal proliferation arrest at the terminal differentiation: PATF (Proliferation Arrest Transcription Factor). We show that PATF associates with E2F4 protein and interacts with the E2F consensus site. PATF expression increases with establishment of quiescent state. Furthermore, the nuclear PATF localization like E2F4, depends on cell growth arrest. The decrease of PATF amount, using a retroviral antisense strategy, results in pursued neuroretina cell mitosis. Our results indicate that PATF could be a new molecular signal implicated in the final neuronal cell cycle withdrawal.
Crisanti
P
P
Développement, Vieillissement et Pathologie de la Rétine, INSERM U450, Affiliée CNRS, Association Claude Bernard, 29 rue Wilhem, 75016 Paris, France. plassiaz@infobiogen.fr
Raguenez
G
G
Blancher
C
C
Neron
B
B
Mamoune
A
A
Omri
B
B
D000595:Amino Acid Sequence; D000818:Animals; D030161:Avian Proteins; D001483:Base Sequence; D050976:Basic-Leucine Zipper Transcription Factors; D002455:Cell Division; D002467:Cell Nucleus / Q000378:metabolism; D002642:Chick Embryo; D003001:Cloning, Molecular; D016384:Consensus Sequence; D016373:DNA, Antisense / Q000494:pharmacology; D004268:DNA-Binding Proteins / Q000378:metabolism; D050704:E2F4 Transcription Factor; D005136:Eye Proteins / Q000235:genetics / Q000502:physiology; D006801:Humans; D008969:Molecular Sequence Data; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D016716:PC12 Cells; D012333:RNA, Messenger / Q000096:biosynthesis; D011817:Rabbits; D051381:Rats; D012160:Retina / Q000166:cytology / Q000196:embryology*; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D015533:Transcriptional Activation; D014407:Tumor Cells, Cultured
D030161:Avian Proteins; D050976:Basic-Leucine Zipper Transcription Factors; D016373:DNA, Antisense; D004268:DNA-Binding Proteins; D050704:E2F4 Transcription Factor; D005136:Eye Proteins; D012333:RNA, Messenger; D014157:Transcription Factors; C436504:proliferation arrest transcription factor, Coturnix japonica