GC-MS determination of gamma-hydroxybutyric acid (GHB) in blood.
A sensitive and specific gas chromatography-mass spectrometer (GC-MS) method using selective ion monitoring (SIM) has been developed for the quantification of gamma-hydroxybutyric acid (GHB) in blood. This method uses liquid-liquid extraction and disilyl-derivatization, without conversion to the gamma-butyrolactone (GBL), followed by GC-MS analysis using GHB-d6 as the internal standard. The method was linear from 0.1 to 20 mg/dl, and the limit of quantification using 50 microl of blood was determined to be 0.1 mg/dl.
Elian
A A
AA
Commonwealth of Massachusetts, Slate Police Crime Laboratory, Forensic Toxicology Unit, 59 Horsepond Road, Sudbury, MA 01776, USA.
D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D006885:Hydroxybutyrates / Q000097:blood*
D006885:Hydroxybutyrates; C111420:4-hydroxybutyric acid