[Effects of 1-(2,6-dimethylphenoxy)-2-(3,4-dimethoxyphenylethylamino) propane hydrochloride on proliferation of vascular smooth muscle cells and PDGF-B, bFGF, c-sis, c-myc in spontaneously hypertensive rat].
In order to determine the effects of 1-(2, 6-dimethylphenoxy)-2-(3, 4-dimethoxyphenylethylamino) propane hydrochloride(DDPH) on proliferation of vascular smooth muscle cells(VSMC), growth factor PDGF-B, bFGF and expression of oncogene c-sis and c-myc in spontaneously hypertensive rat(SHR), 3H-thymidine(3H-TdR) incorporation, electronic microscopy, immunohistochemistry and in situ hybridization assays were used. The results showed that after DDPH administration (50 mg i.g. q.d. x8 wk to SHR), morphological change of VSMC was antagonized, blood pressure and 3H-TdR incorporation were significantly reduced. Furthermore, DDPH was shown to reverse the enhanced antigen PDGF-B and bFGF and reverse the reduced expression of oncogene c-sis and c-myc in SHR. These results suggest that DDPH may inhibit VSMC proliferation in SHR, which may be relevant to the molecular biological mechanism of growth factors and oncogene control.
Xiong
Y L
YL
Department of Pharmacology, Tongji Medical University, Wuhan 430030.
Qian
J Q
JQ
D000818:Animals; D000959:Antihypertensive Agents / Q000494:pharmacology; D001794:Blood Pressure / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D016222:Fibroblast Growth Factor 2 / Q000096:biosynthesis*; D015870:Gene Expression / Q000187:drug effects; D016259:Genes, myc; D020571:Genes, sis; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000166:cytology*; D010627:Phenethylamines / Q000494:pharmacology*; D020574:Proto-Oncogene Proteins c-sis / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011897:Random Allocation; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D012077:Renal Artery / Q000166:cytology
D000959:Antihypertensive Agents; D010627:Phenethylamines; D020574:Proto-Oncogene Proteins c-sis; D012333:RNA, Messenger; D016222:Fibroblast Growth Factor 2; C057485:1-(2,6-dimethylphenoxy)-2-(3,4-dimethoxyphenylethylamino)propane