Determination of selamectin in dog plasma by high performance liquid chromatography with automated solid phase extraction and fluorescence detection.
A method is described for the determination of selamectin in dog plasma, using High-Performance Liquid Chromatography (HPLC) with fluorescence detection (excitation and emission wavelengths fixed at 355 and 465 nm, respectively). The fluorescent derivative was obtained by condensation reaction with trifluoroacetic anhydride and N-methylimidazole. The method employs 1 mL plasma samples and gives linear calibration graphs (r = 0.999) over the concentration range studied (0.5-50 ng mL(-1)). Automatic solid phase extraction using the benchmate procedure was used for sample preparation. This method permits the determination of selamectin at levels as low as 0.1 ng mL(-1) and its suitability was demonstrated by a pharmacokinetic study on a dog receiving the therapeutic dose (Spot-on administration).
Sutra
J F
JF
Laboratoire de Pharmacologie-Toxicologie, INRA, Toulouse, France.
Cadiergues
M C
MC
Dupuy
J
J
Franc
M
M
Alvinerie
M
M
D000818:Animals; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods / Q000662:veterinary; D004285:Dogs / Q000097:blood* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005453:Fluorescence; D007306:Insecticides / Q000097:blood* / Q000493:pharmacokinetics / Q000627:therapeutic use; D007559:Ivermectin / Q000031:analogs & derivatives* / Q000097:blood* / Q000493:pharmacokinetics / Q000627:therapeutic use; D012680:Sensitivity and Specificity
D007306:Insecticides; D007559:Ivermectin; C414354:selamectin