Hemostatic/fibrinolytic protein changes in C3H/HeN mice infected with Rickettsia conorii--a model for Rocky Mountain spotted fever.
Changes in plasma hemostatic and fibrinolytic proteins were determined during courses of a murine model of fatal and non-fatal Rocky Mountain spotted fever. C3H/HeN mice were infected with Rickettsia conorii and coagulation and histopathologic studies were performed at prescribed periods of time. A significant decrease in plasma factor VIII activity and rise in plasma factor V procoagulant activity correlated with a fatal infection. Factor VII levels were unchanged; factor XI levels dropped early in the course in the lethally infected animals, but returned to normal. Factor XII, high molecular weight kininogen, and prekallikrein levels were unchanged by the sublethal infection. Prekallikrein levels fell during the lethal infection. Antithrombin concentrations were decreased significantly in all animals, but plasma plasminogen levels did not change in either group of animals. Nonocclusive thrombi were microscopically observed rarely and only in animals surviving a sublethal infection. A fall in tissue plasminogen activator activity and a rise in plasminogen activator inhibitor activity highly correlated with a lethal outcome. Lethal infection with R. conorii is associated with primary endothelial cell injury resulting in decreased tissue plasminogen activator and increased plasminogen activator inhibitor.
Schmaier
A H
AH
Department of Internal Medicine, University of Michigan, Ann Arbor 48109-0640, USA. aschmaie@umich.edu
Srikanth
S
S
Elghetany
M T
MT
Normolle
D
D
Gokhale
S
S
Feng
H M
HM
Walker
D H
DH
D000818:Animals; D001779:Blood Coagulation Factors / Q000378:metabolism; D002642:Chick Embryo; D004730:Endothelium, Vascular / Q000378:metabolism / Q000382:microbiology / Q000473:pathology*; D005165:Factor V / Q000032:analysis; D005342:Fibrinolysis; D006487:Hemostasis; D007928:Lethal Dose 50; D051379:Mice; D008809:Mice, Inbred C3H; D023421:Models, Animal; D010314:Partial Thromboplastin Time; D017395:Plasminogen Activator Inhibitor 1 / Q000032:analysis; D011517:Prothrombin Time; D020114:Rickettsia conorii / Q000502:physiology*; D012373:Rocky Mountain Spotted Fever / Q000097:blood* / Q000150:complications; D013047:Specific Pathogen-Free Organisms; D019851:Thrombophilia / Q000209:etiology / Q000473:pathology; D010959:Tissue Plasminogen Activator / Q000032:analysis
D001779:Blood Coagulation Factors; D017395:Plasminogen Activator Inhibitor 1; D005165:Factor V; D010959:Tissue Plasminogen Activator