Pythium perplexum isolated from soil in France: morphology, molecular characterisation and biological control.
Pythium perplexum (F-926) was isolated from a soil sample taken in the Burgundy region in France. In 1907, it was mistakenly described by Bulter as P. vexans. Despite morphological resemblance, the comparison between the internal transcribed spacer 1 regions of the ribosomal DNA of the two fungi leaves no doubt of their different identities. P. perplexum was found to be highly pathogenic to cucumber seedlings. Damping-off disease of cucumber caused by P. perplexum can effectively be controlled by using the soil bacterium Serratia plymuthica (B-781). The details of the morphology and the molecular characterisation of P. perplexum and its biological control with S. plymuthica are described here.
Galland
D
D
Laboratoire de Microbiologie, ENSBANA, Université de Bourgogne, I Esplanade Erasme, Dijon, France. gallanddelphine@hotmail.com
Paul
B
B
D000898:Antibiosis; D001483:Base Sequence; D018553:Cucumis sativus / Q000382:microbiology*; D021903:DNA, Ribosomal Spacer / Q000032:analysis; D005602:France; D008969:Molecular Sequence Data; D010572:Pest Control, Biological; D010935:Plant Diseases / Q000382:microbiology; D011775:Pythium / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity / Q000502:physiology; D017422:Sequence Analysis, DNA; D012705:Serratia / Q000254:growth & development*; D012988:Soil Microbiology
D021903:DNA, Ribosomal Spacer