Detection of indigenous Halobacillus populations in damaged ancient wall paintings and building materials: molecular monitoring and cultivation.
Several moderately halophilic gram-positive, spore-forming bacteria have been isolated by conventional enrichment cultures from damaged medieval wall paintings and building materials. Enrichment and isolation were monitored by denaturing gradient gel electrophoresis and fluorescent in situ hybridization. 16S ribosomal DNA analysis showed that the bacteria are most closely related to Halobacillus litoralis. DNA-DNA reassociation experiments identified the isolates as a population of hitherto unknown Halobacillus species.
Piñar
G
G
Institute of Microbiology and Genetics, University of Vienna, A-1030 Vienna, Austria. upe@gem.univie.ac.at
Ramos
C
C
Rölleke
S
S
Schabereiter-Gurtner
C
C
Vybiral
D
D
Lubitz
W
W
Denner
E B
EB
D001317:Austria; D003252:Construction Materials; D003470:Culture Media; D004586:Electrophoresis / Q000379:methods; D006094:Gram-Positive Bacteria / Q000145:classification / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification*; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D010151:Paintings; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
D003470:Culture Media; D012336:RNA, Ribosomal, 16S