title: Human immunodeficiency virus type 1 (HIV-1) non-B subtypes are similar to HIV-1 subtype B in that coreceptor specificity is a determinant of cytopathicity in human lymphoid tissue infected ex vivo.
abstract: We sought to determine the relationship between virus-mediated CD4(+) T-lymphocyte cytopathicity and viral coreceptor preference among various human immunodeficiency virus type 1 (HIV-1) subtypes in an ex vivo-infected human lymphoid tissue model. Our data show that all R5 HIV-1 infections resulted in mild depletion of CD4(+) T lymphocytes, whereas all X4 HIV-1 infections caused severe depletion of CD4(+) T lymphocytes regardless of their subtype origin. Thus, at least for the viruses within subtypes A, B, C, and E that were tested, coreceptor specificity is a critical factor that determines the ability of HIV-1 to deplete CD4(+) T cells in human lymphoid tissue infected ex vivo.
authors.affiliation: Laboratory of Cellular and Molecular Biophysics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA.
authors.forename: N
authors.initials: N
authors.lastname: Malkevich
authors.forename: C
authors.initials: C
authors.lastname: Womack
authors.forename: P
authors.initials: P
authors.lastname: Pandya
authors.forename: J C
authors.initials: JC
authors.lastname: Grivel
authors.forename: A S
authors.initials: AS
authors.lastname: Fauci
authors.forename: L
authors.initials: L
authors.lastname: Margolis
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology / Q000821:virology; D018791:CD4 Lymphocyte Count; D015497:HIV-1 / Q000145:classification* / Q000472:pathogenicity; D006801:Humans; D008221:Lymphoid Tissue / Q000821:virology*; D019713:Receptors, CCR5 / Q000502:physiology*; D019718:Receptors, CXCR4 / Q000502:physiology*
chemical_list: D019713:Receptors, CCR5; D019718:Receptors, CXCR4