title: [Endogenous substance P in corneal epithelial cells and keratocytes].
abstract: OBJECTIVE
  To detect endogenous substance P(SP) and neurokinin receptor 1(NK1R) in cultured human epithelial cells of the cornea(HE) and human keratocytes(HK).
  
  
  METHODS
  Messenger RNA(mRNA) expression of SP and endogenous SP in HE and HK were detected by reverse transcription-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA). mRNA expression of NK1R in HE and HK was detected by RT-PCR.
  
  
  RESULTS
  The mRNA expression of SP and endogenous SP were recognized in HE and HK. The mRNA of NK1R was also expressed in HE and HK.
  
  
  CONCLUSIONS
  It appears that endogenous SP regulates the biological functions of HE and HK in an autocrine or paracrine fashion.
authors.affiliation: Eye Department, Asama Municipal Hospital.
authors.forename: M
authors.initials: M
authors.lastname: Watanabe
authors.forename: K
authors.initials: K
authors.lastname: Nakayasu
authors.forename: M
authors.initials: M
authors.lastname: Iwatsu
authors.forename: A
authors.initials: A
authors.lastname: Kanai
mesh_terms: D000368:Aged; D002478:Cells, Cultured; D019573:Epithelium, Corneal / Q000737:chemistry* / Q000166:cytology; D005260:Female; D006801:Humans; D015603:Keratinocytes / Q000737:chemistry*; D008297:Male; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D018040:Receptors, Neurokinin-1 / Q000032:analysis; D013373:Substance P / Q000032:analysis*
chemical_list: D012333:RNA, Messenger; D018040:Receptors, Neurokinin-1; D013373:Substance P