title: Effects of whole cell sonicates of Treponema lecithinolyticum on osteoclast differentiation.
abstract: BACKGROUND
  Alveolar bone destruction is a characteristic feature of periodontal diseases and multinucleated osteoclast cells derived from hemopoietic cells are responsible for bone resorption. Treponema lecithinolyticum is a novel oral spirochete isolated from the periodontal lesions.
  
  
  METHODS
  The effect of whole cell sonicates on the osteoclast differentiation was examined in a co-culture system of hemopoietic mouse bone marrow cells and calvaria derived-osteoblastic cells to clarify the role of T. lecithinolyticum in the alveolar bone destruction associated with periodontal diseases. The differentiated osteoclasts were confirmed by tartrate-resistant acid phosphatase (TRAP) staining.
  
  
  RESULTS
  Sonicates of this bacterium stimulated the osteoclast formation in the co-culture system in a dose-dependent manner. The sonicates-induced osteoclast formation was partially inhibited by the heat treatment of sonicates. Indomethacin, which is a prostaglandin inhibitor, decreased the osteoclast formation induced by the bacterial sonicates.
  
  
  CONCLUSIONS
  These findings suggest that T. lecithinolyticum induces osteoclast differentiation by a prostaglandin E2-dependent mechanism and that heat-labile components may be involved in this process.
authors.affiliation: Department of Oral Biology, College of Dentistry, and Brain Korea 21 Project for Medical Sciences, Yonsei University, Seoul, Korea.
authors.forename: B K
authors.initials: BK
authors.lastname: Choi
authors.forename: S H
authors.initials: SH
authors.lastname: Ohk
authors.forename: H J
authors.initials: HJ
authors.lastname: Lee
authors.forename: J H
authors.initials: JH
authors.lastname: Kang
authors.forename: G J
authors.initials: GJ
authors.lastname: Jeong
authors.forename: Y J
authors.initials: YJ
authors.lastname: Yoo
mesh_terms: D016301:Alveolar Bone Loss / Q000382:microbiology*; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D001854:Bone Marrow Cells / Q000187:drug effects; D002352:Carrier Proteins / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D018920:Coculture Techniques; D017077:Culture Media, Conditioned / Q000378:metabolism / Q000494:pharmacology; D015232:Dinoprostone / Q000037:antagonists & inhibitors; D004305:Dose-Response Relationship, Drug; D007213:Indomethacin / Q000494:pharmacology; D008562:Membrane Glycoproteins / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D010010:Osteoclasts / Q000187:drug effects*; D011448:Prostaglandin Antagonists / Q000494:pharmacology; D053245:RANK Ligand; D053246:Receptor Activator of Nuclear Factor-kappa B; D013010:Sonication; D018709:Statistics, Nonparametric; D014208:Treponema / Q000472:pathogenicity*
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D002352:Carrier Proteins; D017077:Culture Media, Conditioned; D008562:Membrane Glycoproteins; D011448:Prostaglandin Antagonists; D053245:RANK Ligand; D053246:Receptor Activator of Nuclear Factor-kappa B; C505687:Tnfrsf11a protein, mouse; C505689:Tnfsf11 protein, mouse; D015232:Dinoprostone; D007213:Indomethacin