title: Macrophage tropism of human immunodeficiency virus type 1 isolates from brain and lymphoid tissues predicts neurotropism independent of coreceptor specificity.
abstract: The viral determinants that underlie human immunodeficiency virus type 1 (HIV-1) neurotropism are unknown, due in part to limited studies on viruses isolated from brain. Previous studies suggest that brain-derived viruses are macrophage tropic (M-tropic) and principally use CCR5 for virus entry. To better understand HIV-1 neurotropism, we isolated primary viruses from autopsy brain, cerebral spinal fluid, blood, spleen, and lymph node samples from AIDS patients with dementia and HIV-1 encephalitis. Isolates were characterized to determine coreceptor usage and replication capacity in peripheral blood mononuclear cells (PBMC), monocyte-derived macrophages (MDM), and microglia. Env V1/V2 and V3 heteroduplex tracking assay and sequence analyses were performed to characterize distinct variants in viral quasispecies. Viruses isolated from brain, which consisted of variants that were distinct from those in lymphoid tissues, used CCR5 (R5), CXCR4 (X4), or both coreceptors (R5X4). Minor usage of CCR2b, CCR3, CCR8, and Apj was also observed. Primary brain and lymphoid isolates that replicated to high levels in MDM showed a similar capacity to replicate in microglia. Six of 11 R5 isolates that replicated efficiently in PBMC could not replicate in MDM or microglia due to a block in virus entry. CD4 overexpression in microglia transduced with retroviral vectors had no effect on the restricted replication of these virus strains. Furthermore, infection of transfected cells expressing different amounts of CD4 or CCR5 with M-tropic and non-M-tropic R5 isolates revealed a similar dependence on CD4 and CCR5 levels for entry, suggesting that the entry block was not due to low levels of either receptor. Studies using TAK-779 and AMD3100 showed that two highly M-tropic isolates entered microglia primarily via CXCR4. These results suggest that HIV-1 tropism for macrophages and microglia is restricted at the entry level by a mechanism independent of coreceptor specificity. These findings provide evidence that M-tropism rather than CCR5 usage predicts HIV-1 neurotropism.
authors.affiliation: Department of Cancer Immunology and AIDS, Dana-Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA.
authors.forename: P R
authors.initials: PR
authors.lastname: Gorry
authors.forename: G
authors.initials: G
authors.lastname: Bristol
authors.forename: J A
authors.initials: JA
authors.lastname: Zack
authors.forename: K
authors.initials: K
authors.lastname: Ritola
authors.forename: R
authors.initials: R
authors.lastname: Swanstrom
authors.forename: C J
authors.initials: CJ
authors.lastname: Birch
authors.forename: J E
authors.initials: JE
authors.lastname: Bell
authors.forename: N
authors.initials: N
authors.lastname: Bannert
authors.forename: K
authors.initials: K
authors.lastname: Crawford
authors.forename: H
authors.initials: H
authors.lastname: Wang
authors.forename: D
authors.initials: D
authors.lastname: Schols
authors.forename: E
authors.initials: E
authors.lastname: De Clercq
authors.forename: K
authors.initials: K
authors.lastname: Kunstman
authors.forename: S M
authors.initials: SM
authors.lastname: Wolinsky
authors.forename: D
authors.initials: D
authors.lastname: Gabuzda
mesh_terms: D000595:Amino Acid Sequence; D001921:Brain / Q000821:virology*; D015704:CD4 Antigens / Q000032:analysis; D015686:Gene Products, env / Q000737:chemistry; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000821:virology; D008221:Lymphoid Tissue / Q000821:virology*; D008264:Macrophages / Q000821:virology*; D017628:Microglia / Q000821:virology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D019713:Receptors, CCR5 / Q000032:analysis / Q000502:physiology; D019718:Receptors, CXCR4 / Q000502:physiology; D015728:Receptors, HIV / Q000502:physiology*; D014779:Virus Replication
chemical_list: D015704:CD4 Antigens; D015686:Gene Products, env; D019713:Receptors, CCR5; D019718:Receptors, CXCR4; D015728:Receptors, HIV