Anticonvulsant activities of ethanolic extract and aqueous fraction isolated from Delphinium denudatum.
Dried roots of Delphinium denudatum Wall. are a popular folk remedy for the treatment of epilepsy in the traditional Unani system of medicine in the sub-continent. We carried out anticonvulsant screening of the ethanolic extract (EE) and aqueous fraction (AF) of this plant utilising the maximal electroshock (MEST) and subcutaneous pentylenetetrazole (scPTZ), bicuculline (scBIC), picrotoxin (scPTX) and strychnine (scSTN) tests for anticonvulsant activity. EE had weak dose-dependent anticonvulsant effects on seizures induced by PTZ and BIC. AF exhibited dose-dependent activity against hind limb tonic extension phase (HLTE) of MEST and comparatively stronger anticonvulsant activity against seizures induced by PTZ and BIC. The results suggest the presence of potent anticonvulsant compounds in AF of D. denudatum and deserve further investigation for isolation of active compounds and elucidation of the mechanism of anticonvulsant action.
Raza
M
M
Department of Neurology, Medical College of Virginia, Virginia Commonwealth University, P.O. Box 980599, Richmond, VA 23298-0599, USA.
Shaheen
F
F
Choudhary
M I
MI
Sombati
S
S
Rafiq
A
A
Suria
A
A
Rahman
A
A
DeLorenzo
R J
RJ
D000818:Animals; D000927:Anticonvulsants / Q000494:pharmacology*; D001640:Bicuculline / Q000494:pharmacology; D003292:Convulsants / Q000494:pharmacology; D004597:Electroshock; D000431:Ethanol; D008297:Male; D051379:Mice; D010433:Pentylenetetrazole / Q000037:antagonists & inhibitors / Q000633:toxicity; D010852:Picrotoxin / Q000494:pharmacology; D029626:Ranunculaceae / Q000737:chemistry*; D012997:Solvents; D013331:Strychnine / Q000494:pharmacology; D014867:Water
D000927:Anticonvulsants; D001640:Bicuculline; D003292:Convulsants; D000431:Ethanol; D010433:Pentylenetetrazole; D010852:Picrotoxin; D012997:Solvents; D013331:Strychnine; D014867:Water