title: Co-expression of P2X receptor subunits on rat nodose neurons that bind the isolectin GS-I-B4.
abstract: Triple fluorescent histochemistry was used to describe the types of overlap in visceral sensory neurons (nodose ganglion) for the labeling of the isolectin B4 from Griffonia simplicifolia type one (GS-I-B4) and their immunoreactivity (IR) for two of the ATP receptor subunits (P2X1/3 or P2X2/3). The vast majority of nodose neurons expressed GS-I-B4-binding and most of these displayed P2X receptor IR. Most of the P2X-IR was co-expressed on these individual nodose neurons (P2X1/P2X3 or P2X2/P2X3). A very small subpopulation of neurons that were GS-I-B4 negative but P2X positive displayed a very high relative intensity of P2X3-IR. The functional role that these expression patterns play in visceral sensory processing is currently unclear.
authors.affiliation: Department of Physiological Sciences, College of Veterinary Medicine and The Evelyn F. And William L. McKnight Brain Institute, University of Florida, Gainesville, Box 100144, FL 32610-0144, USA.
authors.forename: C H
authors.initials: CH
authors.lastname: Hubscher
authors.forename: J C
authors.initials: JC
authors.lastname: Petruska
authors.forename: K K
authors.initials: KK
authors.lastname: Rau
authors.forename: R D
authors.initials: RD
authors.lastname: Johnson
mesh_terms: D000818:Animals; D001665:Binding Sites / Q000187:drug effects / Q000502:physiology; D005260:Female; D007150:Immunohistochemistry; D037102:Lectins / Q000493:pharmacokinetics*; D009475:Neurons, Afferent / Q000166:cytology / Q000378:metabolism*; D009619:Nociceptors / Q000166:cytology / Q000378:metabolism*; D009620:Nodose Ganglion / Q000166:cytology / Q000378:metabolism*; D010146:Pain / Q000378:metabolism* / Q000503:physiopathology; D051381:Rats; D018048:Receptors, Purinergic P2 / Q000378:metabolism*; D058469:Receptors, Purinergic P2X; D058476:Receptors, Purinergic P2X2; D058477:Receptors, Purinergic P2X3; D017833:Visceral Afferents / Q000166:cytology / Q000378:metabolism*
chemical_list: D037102:Lectins; C546403:P2rx2 protein, rat; C546410:P2rx3 protein, rat; D018048:Receptors, Purinergic P2; D058469:Receptors, Purinergic P2X; D058476:Receptors, Purinergic P2X2; D058477:Receptors, Purinergic P2X3