title: Characterization of a hypervariable region in the genome of Chlamydophila pneumoniae.
abstract: Chlamydophila pneumoniae displays surprisingly little genomic variation, as seen by comparisons of the published genomes from three different isolates and sequencing of four different genes from different isolates. We have in the present study, however, demonstrated genomic variation between 10 C. pneumoniae isolates in the 11690-bp region between the two outer membrane protein genes pmp1 and pmp2. This region of the C. pneumoniae CWL-029 isolate contains seven C. pneumoniae-specific open reading frames (hb1-7, encoding hydrophobic beta-sheet-containing proteins). We identified additionally 12 open reading frames in the C. pneumoniae CWL-029 genome encoding hypothetical proteins with similarity to the seven hypothetical Hb-proteins. Compared to other isolates, genomic variation is seen to cause frame-shifting of three of the 19 hb-open reading frames, which are proposed to be three full-length genes and eight frame-shifted pseudogenes. The hypothetical proteins encoded by these proposed genes contain an N-terminally located highly hydrophobic stretch of 50-60 residues. A similar motif is found in all identified Chlamydia inclusion membrane proteins and therefore the Hb-proteins are candidate inclusion proteins.
authors.affiliation: Department of Medical Microbiology and Immunology, The Bartholin Building, University of Aarhus, DK-8000 Aarhus C, Denmark.
authors.forename: L
authors.initials: L
authors.lastname: Daugaard
authors.forename: G
authors.initials: G
authors.lastname: Christiansen
authors.forename: S
authors.initials: S
authors.lastname: Birkelund
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D001483:Base Sequence; D016993:Chlamydophila pneumoniae / Q000145:classification* / Q000235:genetics*; D004262:DNA Restriction Enzymes / Q000378:metabolism; D021901:DNA, Intergenic / Q000235:genetics*; D014644:Genetic Variation / Q000235:genetics*; D016680:Genome, Bacterial; D006801:Humans; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
chemical_list: D001425:Bacterial Outer Membrane Proteins; D021901:DNA, Intergenic; D004262:DNA Restriction Enzymes