Development of sensitive high-performance liquid chromatography with fluorescence detection using 4-(4,5-diphenyl-1H-imidazol-2-yl)-benzoyl chloride as a labeling reagent for determination of bisphenol A in plasma samples.
A sensitive HPLC method for determination of bisphenol A (BPA) in plasma samples using 4-(4,5-diphenyl-1H-imidazol2-yl)benzoyl chloride (DIB-Cl) as a fluorescence labeling reagent was developed. The fluorescence labeling reaction was completed within 10 min at room temperature. DIB-Cl reacts with the phenolic hydroxyl group of BPA in the presence of triethylamine (TEA). The DIB-Cl derivative of BPA (DIB-BPA) was separated within 30 min with an ODS column using acetonitrile-water (90:10, v/v) as the isocratic eluent. Calibration graphs were linear over the range of 1.0-100 ng/ml (r=0.999). The detection limit of DIB-BPA was 0.05 ng/ml (2.5 pg) at a signal-to-noise ratio of 3. The relative standard deviations (RSDs) of the method for between-run were 1.0-5.0%. The analytical recoveries of known amounts (1.0 and 100 ng/ml) of BPA-spiked rabbit plasma were around 95%.
Watanabe
T
T
Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Hoshi University, Tokyo, Japan.
Yamamoto
H
H
Inoue
K
K
Yamaguchi
A
A
Yoshimura
Y
Y
Kato
K
K
Nakazawa
H
H
Kuroda
N
N
Nakashima
K
K
D000818:Animals; D001559:Benzhydryl Compounds; D001565:Benzoates / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry*; D007093:Imidazoles / Q000737:chemistry*; D008297:Male; D010636:Phenols / Q000097:blood*; D011817:Rabbits; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D001565:Benzoates; D005456:Fluorescent Dyes; D007093:Imidazoles; D010636:Phenols; C114684:4-(4,5-diphenyl-1H-imidazol-2-yl)benzoyl chloride; C006780:bisphenol A; D000098826:Bisphenol A Compounds