Sialylation is modulated through maturation in hemocytes from Macrobrachium rosenbergii.
In this work we identified in adult and juvenile freshwater prawn, Macrobrachium rosenbergii, three major type of circulating hemocytes: fusiform; rounded; and large ovoid hemocytes. Rounded and large hemocytes represent the first defense line, since this type of cells exerts phagocytic activity as well as lectin synthesis. Considering that glycosylation plays important roles in cell communication and as a target for pathogenic microorganisms, in this report was also described the main glycosidic modifications that occur in the large and rounded hemocytes from the freshwater prawn during maturation as determined with lectins. Neu5Acalpha2,6Gal, was identified homogeneously distributed in the membrane in 90% of hemocytes from juvenile organisms. Maturation of the freshwater prawn induced a decrease or complete loss of Neu5Acalpha2,6Gal residues that were replaced with Neu5Acalpha2,3 molecules in practically all hemocytes from adult organisms. This change was paralleled by a diminution in 9-O-acetyl-neuraminic acid (Neu5,9Ac(2)) expression. T and Tn antigens (Galbetal,3 GalNAcalpha1-0-Ser/Thr or GalNAcalpha1-0-Ser/Thr, respectively), as well as N-glycosidically linked glycans, seem to be highly conserved throughout maturation. Our results show that sialylation of freshwater prawn hemocytes is modulated throughout the maturation process.
Sierra
C
C
Laboratorio de Lectinas, Centro de Investigaciones Químicas, Universidad Autónoma del Estado de Morelos, Morelos. 62210, Cuernavaca, Mexico.
Guevara
J
J
Lascurain
R
R
Pérez
A
A
Agundis
C
C
Zenteno
E
E
Vázquez
L
L
D000818:Animals; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000378:metabolism; D000957:Antigens, Viral, Tumor / Q000378:metabolism; D006027:Glycosides / Q000378:metabolism*; D006031:Glycosylation; D006434:Hemocytes / Q000378:metabolism* / Q000648:ultrastructure; D037102:Lectins / Q000378:metabolism*; D008855:Microscopy, Electron, Scanning; D019158:N-Acetylneuraminic Acid / Q000097:blood*; D033541:Palaemonidae / Q000145:classification / Q000378:metabolism*; D010587:Phagocytosis / Q000502:physiology; D011134:Polysaccharides / Q000378:metabolism; D013194:Staining and Labeling
D015295:Antigens, Tumor-Associated, Carbohydrate; D000957:Antigens, Viral, Tumor; D006027:Glycosides; D037102:Lectins; D011134:Polysaccharides; C040286:Tn antigen; D019158:N-Acetylneuraminic Acid