Regulation of phenobarbital-mediated induction of CYP102 (cytochrome P450(BM-3)) in Bacillus megaterium by phytochemicals from soy and green tea.
Cytochrome P450 102 (CYP102 or Cytochrome P450(BM)(-)(3)) is induced in Bacillus megaterium by barbiturates, perioxisome proliferators, estrogen, and nonsteroidal antiinflammatory drugs. We have previously demonstrated that a CYP102 construct (BMC 143) coupled with a luciferase reporter gene can be used to identify the inducers of CYP102. We now describe the effect of added phytochemicals on the induction of CYP102 by phenobarbital (PB) in B. megaterium. The isoflavones genistein, biochanin A, coumestrol, and equol, the green tea flavanoid epicatechin, and the fungal toxin zearalenone inhibit the induction of CYP102 by PB in a dose-dependent manner. However, the isoflavone daidzein, the phytoalexin glyceollin, and catechin, an epimer of epicatechin, failed to exhibit a similar inhibitory effect on PB-mediated CYP102 induction.
Rajnarayanan
R V
RV
Department of Chemistry, Tulane University, New Orleans, Louisiana 70118, USA.
Rowley
C W
CW
Hopkins
N E
NE
Alworth
W L
WL
D001410:Bacillus megaterium / Q000201:enzymology*; D001426:Bacterial Proteins; D002392:Catechin / Q000494:pharmacology; D002839:Chromans / Q000494:pharmacology; D003375:Coumestrol / Q000494:pharmacology; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis*; D060754:Equol; D019833:Genistein / Q000494:pharmacology; D007529:Isoflavones / Q000494:pharmacology; D006899:Mixed Function Oxygenases / Q000096:biosynthesis*; D009251:NADPH-Ferrihemoprotein Reductase; D010634:Phenobarbital / Q000494:pharmacology*; D013025:Glycine max / Q000737:chemistry*; D013662:Tea / Q000737:chemistry*; D015025:Zearalenone / Q000494:pharmacology
C007610:4',7-dihydroxy-3,4-dihydroisoflavone; D001426:Bacterial Proteins; D002839:Chromans; D007529:Isoflavones; D013662:Tea; D060754:Equol; D015025:Zearalenone; C004742:daidzein; D002392:Catechin; D003577:Cytochrome P-450 Enzyme System; D019833:Genistein; D006899:Mixed Function Oxygenases; D009251:NADPH-Ferrihemoprotein Reductase; C052423:flavocytochrome P450 BM3 monoxygenases; C004541:biochanin A; D003375:Coumestrol; D010634:Phenobarbital