title: Dynamically coated capillaries improve the identification power of capillary zone electrophoresis for basic drugs in toxicological analysis.
abstract: In systematic toxicological analysis (STA), analytical methods should have a high identification power. This can be suitably expressed by parameters such as mean list length (MLL) or discriminating power (DP). The reproducibility of a method has a great impact on its identification power, and should be as high as possible. In this study, two separation methods based on capillary zone electrophoresis (CZE) were evaluated towards STA applications. Besides a normal phosphate buffer, the commercially available buffer CElixir was used, which is a double-layer dynamic coating system. The coating stabilizes the endoosmotic flow, is independent of the pH, and is claimed to be more reproducible and faster at low pH than with normal buffers. A test set of 73 basic pharmaceutical compounds was analyzed by the two CZE methods. The total analysis time, including rinsing steps, was 8 min when the coating was used and 18 min without the coating. Effective mobilities were calculated and the reproducibilities were a factor of 2 better when the coating was used (between-days SD 0.020 and 0.040 m2/V s with and without the coating, respectively). MLL and DP were calculated for the two CZE methods and for combinations with standardized liquid and gas chromatography systems. CZE with CElixir coating clearly has a high potential for STA applications, as it was shown to have a higher identification power and shorter analysis times than normal CZE.
authors.affiliation: Department of Analytical Chemistry and Toxicology, University Centre for Pharmacy, State University of Groningen, The Netherlands. c.m.boone@farm.rug.nl
authors.forename: C M
authors.initials: CM
authors.lastname: Boone
authors.forename: E Z
authors.initials: EZ
authors.lastname: Jonkers
authors.forename: J P
authors.initials: JP
authors.lastname: Franke
authors.forename: R A
authors.initials: RA
authors.lastname: de Zeeuw
authors.forename: K
authors.initials: K
authors.lastname: Ensing
mesh_terms: D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D004364:Pharmaceutical Preparations / Q000032:analysis*; D015203:Reproducibility of Results; D014116:Toxicology
chemical_list: D004364:Pharmaceutical Preparations