Increasing the efficiency of virus infectivity assays: small inoculum volumes are as effective as centrifugal enhancement.
Improving the virus particle:infectious unit ratio is a continuing goal for animal virologists. It is demonstrated for an influenza A virus that decreasing the size of the inoculum volume to 10 microl per well of a 96-well plate was as effective as using centrifugal force with inoculum up to 250 microl. Both achieved a 7.5-fold increase in infectivity in monolayers of MDCK cells compared with standard conditions. The underlying principle of both methods is to bring virus particles into close contact with cell receptors.
Reading
S A
SA
Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, UK.
Edwards
M J
MJ
Dimmock
N J
NJ
D000818:Animals; D002460:Cell Line; D002498:Centrifugation; D004285:Dogs; D009980:Influenza A virus / Q000502:physiology*; D007700:Kinetics; D009976:Orthomyxoviridae Infections / Q000821:virology*; D011991:Receptors, Virus / Q000378:metabolism*; D013997:Time Factors; D014771:Virion / Q000254:growth & development*; D014779:Virus Replication
D011991:Receptors, Virus