Axon-Schwann cell interactions regulate the expression of fibroblast growth factor-5 (FGF-5).
We screened for genes whose expression is significantly up- or downregulated during Wallerian degeneration in adult rat sciatic nerve with cDNA arrays. Fibroblast growth factor-5 (FGF-5) mRNA seemed to be induced. This was confirmed by northern blotting and in situ hybridization, as well as Western blotting for FGF-5 in axotomized nerve. Axon-Schwann cell interactions decreased the steady-state level of FGF-5 mRNA in regenerating sciatic nerves, and forskolin diminished its expression in cultured Schwann cells. We conclude that denervated Schwann cells synthesize FGF-5, which is a secreted, neuronotrophic member of the FGF family.
Scarlato
M
M
Neurology Research, The Children's Hospital of Philadelphia, Philadelphia, Pennsylvania 19104, USA.
Xu
T
T
Bannerman
P
P
Beesley
J
J
Reddy
U R
UR
Rostami
A
A
Scherer
S S
SS
Pleasure
D
D
D000818:Animals; D001369:Axons / Q000502:physiology*; D019771:Axotomy; D002450:Cell Communication / Q000502:physiology; D002478:Cells, Cultured; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism; D051521:Fibroblast Growth Factor 5; D005346:Fibroblast Growth Factors / Q000235:genetics*; D015870:Gene Expression / Q000187:drug effects / Q000502:physiology; D009416:Nerve Regeneration / Q000502:physiology*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D011917:Rats, Inbred Lew; D012583:Schwann Cells / Q000166:cytology / Q000502:physiology*; D012584:Sciatic Nerve / Q000166:cytology / Q000502:physiology*; D014855:Wallerian Degeneration / Q000503:physiopathology
C496451:Fgf5 protein, rat; D012333:RNA, Messenger; D051521:Fibroblast Growth Factor 5; D005576:Colforsin; D005346:Fibroblast Growth Factors; D000242:Cyclic AMP