Dietary fat up-regulates the apolipoprotein E mRNA level in the Zucker lean rat brain.
High-fat diet alters apo E-dependent processing of beta-amyloid precursor protein. Here we have evaluated the effects of dietary fat on brain apo E mRNA in Zucker lean and obese rats. After approximately 2 months on a high-fat diet, there was significant up-regulation of brain apo E mRNA in the Zucker lean rat in parallel with weight gain. Densitometric quantification revealed a 17% increase in apo E mRNA in the brains of lean rats fed high-fat diet compared with those of lean rats fed rat chow. No significant difference in brain apo E mRNA of Zucker obese rats fed different diets was found. These results suggest that dietary fat alters brain apo E levels, which may be regulated, in part, through the leptin receptor.
Knight
D S
DS
Department of Cellular Biology and Anatomy, LSUHSC, 1501 Kings Highway, PO Box 33932, Shreveport, LA 71130, USA.
Mahajan
D K
DK
Qiao
X
X
D000544:Alzheimer Disease / Q000178:diet therapy / Q000235:genetics / Q000378:metabolism; D016229:Amyloid beta-Peptides / Q000096:biosynthesis / Q000235:genetics; D000818:Animals; D001057:Apolipoproteins E / Q000235:genetics*; D001069:Appetite Regulation / Q000235:genetics*; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D001923:Brain Chemistry / Q000235:genetics; D002352:Carrier Proteins / Q000378:metabolism; D004041:Dietary Fats / Q000378:metabolism*; D004435:Eating / Q000235:genetics; D005526:Food, Formulated / Q000009:adverse effects; D020738:Leptin / Q000378:metabolism; D009765:Obesity / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D011924:Rats, Zucker / Q000033:anatomy & histology / Q000378:metabolism*; D011956:Receptors, Cell Surface; D054411:Receptors, Leptin; D015854:Up-Regulation / Q000235:genetics*; D015430:Weight Gain / Q000235:genetics
D016229:Amyloid beta-Peptides; D001057:Apolipoproteins E; D002352:Carrier Proteins; D004041:Dietary Fats; D020738:Leptin; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D054411:Receptors, Leptin