The use of a neutralizing monoclonal antibody to detect infections of equine herpesvirus type 2 (EHV-2).
A blocking enzyme-linked immunosorbent assay (ELISA) was developed to detect antibodies to equine herpesvirus 2 in serum samples of horses. By measuring the binding to a single epitope, this blocking ELISA gives a good picture of the antibody status in the animal. The test is based on a monoclonal antibody with neutralizing activity and had a sensitivity of 94% and a specificity of 100%. Antibodies due to newly acquired infection in foals were successfully detected with this blocking ELISA.
Nordengrahn
A
A
SVANOVA Biotech, National Veterinary Institute (SVA), Uppsala Science Park, Sweden.
Klingeborn
B
B
Lindholm
A
A
Merza
M
M
D000818:Animals; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral / Q000032:analysis*; D018929:Cell Culture Techniques; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D005260:Female; D006566:Herpesviridae Infections / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*; D006736:Horses; D008297:Male; D011817:Rabbits; D018144:Rhadinovirus / Q000276:immunology*; D012680:Sensitivity and Specificity; D014412:Tumor Virus Infections / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral