Micellar electrokinetic capillary chromatography as an alternative method for the determination of dexamethasone, trimethoprim, and polymyxin B.
A micellar electrokinetic capillary chromatographic method is presented which enables quantification of dexamethasone, polymyxin B and trimethoprim in synthetic mixtures and pharmaceutical products. Separation was carried out at 25 degrees C and 30 kV, with 10 mmol L(-1) borate-phosphate buffer adjusted to pH 8 as electrolyte, with 50 mmol L(-1) sodium dodecyl sulfate. Under these conditions separations were performed in 10 min. The limits of detection and quantification were approximately 2 mg L(-1) for each component, except for polymyxin B. The method was applied to different commercial formulations.
Lemus Gallego
J M
JM
Department of Analytical Chemistry and Food Technology, Facultad de Química, Universidad de Castilla-La Mancha, Ciudad Real, Spain. jmlemus@qata-cr.uclm.es
Perez Arroyo
J
J
D000900:Anti-Bacterial Agents / Q000032:analysis; D000892:Anti-Infective Agents, Urinary / Q000032:analysis; D002138:Calibration; D002626:Chemistry, Pharmaceutical; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D003907:Dexamethasone / Q000032:analysis*; D005938:Glucocorticoids / Q000032:analysis; D011112:Polymyxin B / Q000032:analysis*; D011786:Quality Control; D014295:Trimethoprim / Q000032:analysis*
D000900:Anti-Bacterial Agents; D000892:Anti-Infective Agents, Urinary; D005938:Glucocorticoids; D003907:Dexamethasone; D014295:Trimethoprim; D011112:Polymyxin B