Gene-trap mutagenesis: past, present and beyond.
Although at least 35,000 human genes have been sequenced and mapped, adequate expression or functional information is available for only approximately 15% of them. Gene-trap mutagenesis is a technique that randomly generates loss-of-function mutations and reports the expression of many mouse genes. At present, several large-scale, gene-trap screens are being carried out with various new vectors, which aim to generate a public resource of mutagenized embryonic stem (ES) cells. This resource now includes more than 8,000 mutagenized ES-cell lines, which are freely available, making it an appropriate time to evaluate the recent advances in this area of genomic technology and the technical hurdles it has yet to overcome.
Stanford
W L
WL
Programme in Development and Fetal Health, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Room 983, Toronto, Ontario, Canada M5G 1X5. stanford@mshri.on.ca
Cohn
J B
JB
Cordes
S P
SP
D000818:Animals; D002678:Chimera / Q000235:genetics; D004274:DNA, Recombinant / Q000008:administration & dosage / Q000235:genetics; D004331:Drosophila melanogaster / Q000235:genetics; D018274:Electroporation; D004622:Embryo, Mammalian / Q000166:cytology; D004625:Embryo, Nonmammalian; D004742:Enhancer Elements, Genetic / Q000235:genetics; D005544:Forecasting; D015723:Gene Library; D018390:Gene Targeting; D005796:Genes / Q000187:drug effects / Q000528:radiation effects; D017930:Genes, Reporter; D005822:Genetic Vectors / Q000008:administration & dosage / Q000235:genetics; D007763:Lac Operon; D051379:Mice; D008817:Mice, Mutant Strains / Q000235:genetics*; D008822:Mice, Transgenic; D008845:Microinjections; D016254:Mutagenesis, Insertional / Q000379:methods*; D016297:Mutagenesis, Site-Directed; D009153:Mutagens / Q000494:pharmacology; D011401:Promoter Regions, Genetic / Q000235:genetics; D012190:Retroviridae / Q000235:genetics; D013234:Stem Cells
D004274:DNA, Recombinant; D009153:Mutagens