The pattern of gene expression in mouse Gr-1(+) myeloid progenitor cells.
To understand the pattern of gene expression in mouse myeloid progenitor cells, we carried out a genome-wide analysis of gene expression in mouse bone marrow Gr-1(+) cells using SAGE and GLGI techniques. We identified 22,033 unique SAGE tags with quantitative information from 73,869 collected SAGE tags. Among these unique tags, 64% match known sequences, including many genes important for myeloid differentiation, and 36% have no matches to known sequences and are likely to represent novel genes. We compared the expression of mouse Gr-1(+) and human CD15(+) myeloid progenitor cells and showed that the pattern of gene expression of these two cell populations had some similarities. We also compared the expression of mouse Gr-1(+) myeloid progenitor cells with that of mouse brain tissue and found a highly tissue-specific manner of gene expression in these two samples. Our data provide a basis for studying altered gene expression in myeloid disorders using mouse models.
Chen
J
J
Department of Medicine, University of Chicago, 5841 S. Maryland, MC2115, Chicago, Illinois 60637, USA.
Rowley
D A
DA
Clark
T
T
Lee
S
S
Zhou
G
G
Beck
C
C
Rowley
J D
JD
Wang
S M
SM
D000818:Animals; D000943:Antigens, Differentiation / Q000032:analysis; D001921:Brain / Q000378:metabolism; D002454:Cell Differentiation; D018076:DNA, Complementary; D020224:Expressed Sequence Tags; D005260:Female; D015870:Gene Expression; D020869:Gene Expression Profiling; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D023461:Myeloid Progenitor Cells / Q000378:metabolism*
D000943:Antigens, Differentiation; D018076:DNA, Complementary