title: Magnetic resonance imaging for the evaluation of a novel metastatic orthotopic model of human neuroblastoma in immunodeficient mice.
abstract: Neuroblastoma is the second most common solid tumor in children. So far few tumor models for this cancer have been reported in mice. We have created a murine tumor model for studying human neuroblastoma based on surgical orthotopic implantation in scid mice. Small fragments of subcutaneous tumors of SK-N-BE(2) human neuroblastoma cells expressing enhanced green fluorescent protein were surgically implanted near the left adrenal gland of scid mice. One hundred percent of the animals (n = 21) successfully implanted developed a large retroperitoneal tumor and became moribund between 22 and 57 days after implantation (mean survival time = 41 days). At the time of sacrifice the presence of bone marrow metastasis was detected by RT-PCR for green fluorescent protein in 95% of the cases. The growth of small tumor implants could be easily visualized and quantified by surveillance MR imaging, with a resolution of 117 x 117 x 750 microm in two orthogonal planes allowing accurate volume measurements, as well as assessment of necrosis and tissue invasion. This novel model should be a valuable tool to study the biology and therapeutic approaches to neuroblastoma.
authors.affiliation: Childrens Hospital Los Angeles, Department of Pediatrics, USC Keck School of Medicine, California 90027, USA.
authors.forename: R
authors.initials: R
authors.lastname: Moats
authors.forename: L Q
authors.initials: LQ
authors.lastname: Ma
authors.forename: R
authors.initials: R
authors.lastname: Wajed
authors.forename: Y
authors.initials: Y
authors.lastname: Sugiura
authors.forename: A
authors.initials: A
authors.lastname: Lazaryev
authors.forename: M
authors.initials: M
authors.lastname: Tyszka
authors.forename: R
authors.initials: R
authors.lastname: Jacobs
authors.forename: S
authors.initials: S
authors.lastname: Fraser
authors.forename: M D
authors.initials: MD
authors.lastname: Nelson
authors.forename: Y A
authors.initials: YA
authors.lastname: DeClerck
mesh_terms: D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D004195:Disease Models, Animal; D049452:Green Fluorescent Proteins; D006801:Humans; D008164:Luminescent Proteins / Q000235:genetics; D008279:Magnetic Resonance Imaging / Q000379:methods*; D051379:Mice; D016513:Mice, SCID; D009362:Neoplasm Metastasis; D009368:Neoplasm Transplantation; D009447:Neuroblastoma / Q000473:pathology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
chemical_list: D017931:DNA Primers; D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins