Enzyme-linked immunosorbent assay for detection of Trichinella spiralis antibodies and the surveillance of selected pig breeding farms in the Republic of Korea.
Trichinellosis is a parasitic zoonosis of public health importance. It is caused by Trichinella spiralis which has a wide host range including humans. In the present communication, the ELISA technique was employed on a total of 803 blood samples from 7 selected pig breeding farms in 1996 for diagnosis and surveillance of trichinellosis. Out of the entire 803 samples, nine were found to be suspected while one was positive by ELISA. But western blot analyses employed for further confirmation have shown that all of 10 samples did not react to larval excretory-secretory product antigens. These results indicate that pig breeding farms included in the present study are free from trichinellosis. However, it does not mean Korea is free from trichinellosis since human trichinellosis has recently been reported. The necessity of continued surveillance for trichinellosis in both pigs and wild animals was discussed.
Wee
S H
SH
National Veterinary Research & Quarantine Service, MAF, Anyang 430-824, Korea. wsh@nvrqs.go.kr
Lee
C G
CG
Joo
H D
HD
Kang
Y B
YB
D000818:Animals; D000829:Animals, Domestic; D000909:Antibodies, Helminth / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007723:Korea / Q000453:epidemiology; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis* / Q000453:epidemiology; D017160:Trichinella spiralis / Q000276:immunology*; D014235:Trichinellosis / Q000175:diagnosis* / Q000453:epidemiology / Q000662:veterinary*
D000909:Antibodies, Helminth; D015415:Biomarkers