Submicrometer metallic barcodes.
We synthesized multimetal microrods intrinsically encoded with submicrometer stripes. Complex striping patterns are readily prepared by sequential electrochemical deposition of metal ions into templates with uniformly sized pores. The differential reflectivity of adjacent stripes enables identification of the striping patterns by conventional light microscopy. This readout mechanism does not interfere with the use of fluorescence for detection of analytes bound to particles by affinity capture, as demonstrated by DNA and protein bioassays.
Nicewarner-Pena
S R
SR
Department of Chemistry, Pennsylvania State University, 152 Davey Laboratory, University Park, PA 16802, USA., SurroMed Inc., 2375 Garcia Avenue, Mountain View, CA 94043, USA.
Freeman
R G
RG
Reiss
B D
BD
He
L
L
Pena
D J
DJ
Walton
I D
ID
Cromer
R
R
Keating
C D
CD
Natan
M J
MJ
D000818:Animals; D001671:Biochemistry / Q000379:methods*; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D004563:Electrochemistry; D005453:Fluorescence; D005455:Fluorescent Antibody Technique; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007074:Immunoglobulin G / Q000032:analysis; D008670:Metals; D008853:Microscopy; D008904:Miniaturization; D009693:Nucleic Acid Hybridization / Q000379:methods*; D015345:Oligonucleotide Probes; D055095:Optics and Photonics; D011817:Rabbits; D013698:Templates, Genetic
D007074:Immunoglobulin G; D008670:Metals; D015345:Oligonucleotide Probes