[An initial experiment study on allogenic tissue engineered cartilage].
OBJECTIVE
To investigate the formation of engineered cartilage in vivo with allogenic chondrocytes.


METHODS
Joint cartilage from ewe embryos (Pregnancy: 100 days) was isolated and digested to obtain dissociated chondrocytes. Mixed chondrocytes with biomaterials (Pluronic 127) at the density 50 x 10(6)/ml, then implant the allograft complex subcutaneously. Collect the novel tissue every two weeks along for six months, weigh and stain (HE, Safranin O, Masson's trichrome) the sample tissues individually to evaluate the characteristics of novel tissue.


RESULTS
Novel cartilage can regenerate in allograft animals, with similar histological properties of chondrocytes, aminoglycin and collagen distribution to normal cartilage. The novel cartilage observed histologically showed apparent surrounding inflammatory cells in two and four week and the tissue of cartilage came to mature since the sixth week, with less inflammatory reaction, but not disappeared.


CONCLUSIONS
Novel cartilage can generate in allograft animals with tissue engineering approach, with certain immunoreaction surrounding. Such reaction will be weakened gradually with time.
Xia
W
W
Research Center of Tissue Engineering, Shanghai Second Medical University, Department of Plastic Surgery, Ninth People's Hospital, Shanghai 200011, China.
Cao
Y
Y
Shang
Q
Q
D000818:Animals; D001672:Biocompatible Materials; D001698:Biomedical Engineering; D002358:Cartilage, Articular / Q000166:cytology*; D002455:Cell Division; D002469:Cell Separation; D019902:Chondrocytes / Q000637:transplantation*; D004622:Embryo, Mammalian; D005260:Female; D011247:Pregnancy; D019736:Prostheses and Implants; D012756:Sheep
D001672:Biocompatible Materials