A role for membrane-type serine protease (MT-SP1) in intestinal epithelial turnover.
Membrane type-serine protease 1 (MT-SP1) plays potential roles in the process of invasion and metastasis of carcinomas. In the present study, we cloned a rat MT-SP1 cDNA and investigated the intestinal distribution and proteolytic properties of the enzyme. By in situ hybridization we found the prominent expression of the mRNA in the epithelial layer of the small intestinal upper villi and of the colon, where cells are loosely attached to the basement membrane. When MT-SP1 was expressed in Caco-2, a colonic carcinoma cell line, the protein was localized exclusively on the basolateral side. A secreted form of the enzyme produced in COS-1 cells digested fibronectin and laminin. These findings suggest that MT-SP1 participates in the control of intestinal epithelial turnover by regulating the cell-substratum adhesion.
Satomi
S
S
Laboratory of Nutrition Chemistry, Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan.
Yamasaki
Y
Y
Tsuzuki
S
S
Hitomi
Y
Y
Iwanaga
T
T
Fushiki
T
T
D000595:Amino Acid Sequence; D000818:Animals; D019556:COS Cells; D018938:Caco-2 Cells; D002448:Cell Adhesion / Q000502:physiology; D002462:Cell Membrane / Q000201:enzymology; D016764:Cell Polarity / Q000502:physiology; D003001:Cloning, Molecular; D018076:DNA, Complementary; D006801:Humans; D017403:In Situ Hybridization; D007413:Intestinal Mucosa / Q000166:cytology* / Q000201:enzymology*; D008297:Male; D008969:Molecular Sequence Data; D051381:Rats; D017207:Rats, Sprague-Dawley; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012697:Serine Endopeptidases / Q000737:chemistry / Q000378:metabolism*; D014357:Trypsin / Q000737:chemistry / Q000378:metabolism*
D018076:DNA, Complementary; D011993:Recombinant Fusion Proteins; D012697:Serine Endopeptidases; C485593:St14 protein, rat; C485595:matriptase; C485596:ST14 protein, human; D014357:Trypsin