title: Isolation and characterization of Borrelia parkeri in Ornithodoros parkeri (Ixodida: Argasidae) collected in Colorado.
abstract: This study describes the identification of Borrelia parkeri spirochetes in Colorado. Two isolates of B. parkeri (6230 and 6232) were recovered from Ornithodoros parkeri Cooley ticks collected at an inactive prairie dog town in Moffat County. Both isolates were partially characterized by sequencing and subsequent parsimony and neighbor-joining analyses of appropriate regions of the 16S ribosomal RNA, flagellin and P66 genes. Analyses of the 16S gene sequences from the Colorado isolates indicated that they were more closely related to B. parkeri and B. tucatae than to B. hermsii or the other species of Borrelia investigated in this study. Additional analyses of amino acid sequences for flagellin and P66, however, clearly demonstrated that isolates 6230 and 6232 were most closely related to B. parkeri. The possible significance of B. parkeri as an agent of human disease is discussed.
authors.affiliation: Division of Vector-Borne Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, CO 80521, USA. klg0@cdc.gov
authors.forename: K L
authors.initials: KL
authors.lastname: Gage
authors.forename: M E
authors.initials: ME
authors.lastname: Eggleston
authors.forename: R D
authors.initials: RD
authors.lastname: Gilmore
authors.forename: M C
authors.initials: MC
authors.lastname: Dolan
authors.forename: J A
authors.initials: JA
authors.lastname: Montenieri
authors.forename: D T
authors.initials: DT
authors.lastname: Tanda
authors.forename: J
authors.initials: J
authors.lastname: Piesman
mesh_terms: D000818:Animals; D001096:Arachnid Vectors / Q000382:microbiology*; D001426:Bacterial Proteins; D001483:Base Sequence; D001898:Borrelia / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D003120:Colorado; D004269:DNA, Bacterial; D005260:Female; D005408:Flagellin / Q000235:genetics; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008969:Molecular Sequence Data; D026861:Ornithodoros / Q000382:microbiology*; D018272:Porins / Q000235:genetics; D012329:RNA, Bacterial / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000032:analysis
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; C107893:Oms66 protein, Borrelia; D018272:Porins; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D005408:Flagellin