title: Cytotoxic and anti-inflammatory activity of labdane and cis-clerodane type diterpenes.
abstract: Two labdane type diterpenes, labd-13(E)-ene,-8alpha,15-diol (1) and labd-13(E)-ene,-8alpha,15-yl acetate (2) were isolated from the hexane extract of Cistus creticus subsp. eriocephalus (Viv.) Greuter & Burdet leaves, while (+)-19-acetoxy-cis-clerodan-3-en-15-oic acid (3) was isolated from the hexane extract of Cistus monspeliensis L. leaves. The compounds were examined for their in vitro cytostatic and cytotoxic activity against nine human leukemic cell lines, three of which exhibited a multidrug resistant phenotype. They were also evaluated for their anti-inflammatory activity in vivo on the back of hairless mice. The cytostatic and cytotoxic activity of the tested diterpenes followed the order 1>2>3. Topical application of the diterpenes on barrier disrupted skin did not seem to have a significant contribution to the repair rate of the skin barrier.
authors.affiliation: School of Pharmacy, Department of Pharmacognosy, University of Athens, 15771 Athens, Greece. demetzos@galenos.pharm.uoa.gr
authors.forename: C
authors.initials: C
authors.lastname: Demetzos
authors.forename: K
authors.initials: K
authors.lastname: Dimas
authors.forename: S
authors.initials: S
authors.lastname: Hatziantoniou
authors.forename: T
authors.initials: T
authors.lastname: Anastasaki
authors.forename: D
authors.initials: D
authors.lastname: Angelopoulou
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology*; D002460:Cell Line; D030601:Cistaceae / Q000737:chemistry*; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D051379:Mice; D010936:Plant Extracts / Q000494:pharmacology; D018520:Plant Shoots / Q000737:chemistry; D012867:Skin; D014407:Tumor Cells, Cultured
chemical_list: C440094:19-acetoxy-cis-clerodan-3-en-15-oic acid; D000894:Anti-Inflammatory Agents, Non-Steroidal; D000972:Antineoplastic Agents, Phytogenic; D004224:Diterpenes; D010936:Plant Extracts; C440092:labd-13(E)-ene,-8alpha,15-diol; C440093:labd-13(E)-ene,-8alpha,15-yl acetate