Sporadic testicular germ cell cancers do not exhibit specific alteration in CAG/CTG repeats containing genes expressed in human testis.
CAG/CTG repeat expansions in genomic DNA of testicular tumour cell lines, and germline DNA from members of families predisposed to this malignancy, have been previously described. In order to identify genes possibly concerned by this alteration, we attempted to clone all possible human testis cDNA containing at least five CAG/CTG repeats. Thirty-four different transcripts were identified. By using PCR and non denaturing gel electrophoresis, we determined the size of their repeats, as well as their polymorphisms in a collection of human testicular germ cell tumours and the normal surrounding tissues. For all tested genes, we detected the presence of several species of the same mRNA for each person. Nine genes exhibited specific patterns of expression among different groups of individuals, indicative of polymorphism. None of these polymorphisms was related to human testicular tumours.
Ono
Y
Y
Unité 99 INSERM, Hôpital Henri Mondor, 94010 Créteil, France.
Rajpert De-Meyts
E
E
Guellaën
G
G
Bulle
F
F
D020132:Anticipation, Genetic; D004273:DNA, Neoplasm / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D005796:Genes; D018237:Germinoma / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008297:Male; D011110:Polymorphism, Genetic; D012333:RNA, Messenger / Q000096:biosynthesis; D012334:RNA, Neoplasm / Q000096:biosynthesis; D013736:Testicular Neoplasms / Q000235:genetics* / Q000378:metabolism; D013737:Testis / Q000378:metabolism*; D014158:Transcription, Genetic; D019680:Trinucleotide Repeat Expansion
D004273:DNA, Neoplasm; D012333:RNA, Messenger; D012334:RNA, Neoplasm