Percutaneous cutting needle biopsies for histopathological assessment and sperm retrieval in men with azoospermia.
BACKGROUND
Twenty-three men (45 testes) with azoospermia underwent percutaneous testicular biopsy under local anaesthesia.


METHODS
In all but one of the 45 testes two biopsies were taken close to each other, one with a 16 gauge (n = 44) and another with a 14 gauge (n = 45) cutting needle, both with a 19 mm notch. Three quarters of the tissue was used for histopathological assessment and one quarter for direct microscopy.


RESULTS
The histopathological findings were similar between the two needles. The observations with direct microscopy corresponded with the histopathological assessments concerning the presence of mature spermatids in 41 of 45 (91%) biopsies using the 14 gauge and in 40 of 44 (91%) biopsies using the 16 gauge needle. There were no post-operative complications except for minimal pain and minor local swelling.


CONCLUSIONS
Percutaneous material retrieved using 16 gauge and 14 gauge needles is sufficient for histopathological assessment, and the two needles are equally reliable for testicular sperm retrieval. However, needle biopsy with one puncture may not be representative of the entire testis.
Rosenlund
B
B
Department of Obstetrics and Gynaecology, Andrology Centre, Huddinge University Hospital, Uppsala, Sweden. bjorn.rosenlund@obgyn.hs.sll.se
Kvist
U
U
Plöen
L
L
Ekström
U
U
Hovatta
O
O
D000328:Adult; D001707:Biopsy, Needle / Q000295:instrumentation*; D016922:Cellular Senescence; D004867:Equipment Design; D006801:Humans; D008297:Male; D009339:Needles; D009845:Oligospermia / Q000473:pathology*; D013087:Spermatids / Q000473:pathology / Q000502:physiology; D013094:Spermatozoa / Q000473:pathology; D013737:Testis / Q000473:pathology*; D020858:Tissue and Organ Harvesting / Q000379:methods*