Expression of hMTH1 in the hippocampi of control and Alzheimer's disease.
The oxidized purine nucleoside triphosphatase, hMTH1, has a critical role towards preventing errors caused by oxidized purine nucleoside triphosphates such as 8-oxo-dGTP and 2-hydroxy-dATP. We investigated the immunohistochemical expression of hMTH1 in human hippocampal postmortem tissues representing non-neurological disease and Alzheimer's disease (AD). In the non-neurological subjects the hMTH1 protein was enriched in the stratum lucidum at CA3 corresponding to mossy fiber synapses. In AD subjects, the synaptic immunoreactivities at CA3 were significantly decreased, whereas they tended to be increased at the entorhinal cortex. We suggest that the expression of hMTH1 indicates indirect evidence of oxidative stress and its regulation is regionally differentiated in AD.
Furuta
A
A
Department of Neuropathology, Neurological Institute, Graduate School of Medical Sciences, Kyushu University, 3-1-1, Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
Iida
T
T
Nakabeppu
Y
Y
Iwaki
T
T
D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000201:enzymology* / Q000473:pathology / Q000503:physiopathology; D045643:DNA Repair Enzymes; D005260:Female; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D019599:Mossy Fibers, Hippocampal / Q000201:enzymology* / Q000473:pathology / Q000503:physiopathology; D018384:Oxidative Stress / Q000502:physiology*; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D011687:Purines / Q000378:metabolism*
D011687:Purines; D010744:Phosphoric Monoester Hydrolases; C078207:8-oxodGTPase; D045643:DNA Repair Enzymes