title: Structural analysis of the core region of the lipopolysaccharides from eight serotypes of Klebsiella pneumoniae.
abstract: The core regions of the lipopolysaccharides (LPS) from Klebsiella pneumoniae serotypes O1, O2a, O2a,c, O3, O4, O5, O8, and O12 were analysed using NMR spectroscopy, ESI-MS spectroscopy, and chemical methods. All the LPSs had similar core structures, as shown below, differing only in the number and position of beta-D-galacturonic acid substituents: [carbohydrate structure: see text] where P is H or alpha-Hep, J, K is H or beta-GalA. LPS from all serotypes contained varying proportions of structures having additional or missing phosphate substituents. The core from serotype O1 contained a minor amount of a previously described variant with alpha-DD-Hep-(1-->2)-alpha-DD-Hep-(1-->6)-alpha-GlcN-(1--> replacing the alpha-Hep-(1-->4)-alpha-Kdo-(2-->6)-alpha-GlcN-(1--> component.
authors.affiliation: Institute for Biological Sciences, National Research Council, 100 Sussex Drive, Ottawa, Ont., Canada K1A 0R6. evguenii.vinogradov@nrc.ca
authors.forename: E
authors.initials: E
authors.lastname: Vinogradov
authors.forename: M B
authors.initials: MB
authors.lastname: Perry
mesh_terms: D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D007711:Klebsiella pneumoniae / Q000737:chemistry* / Q000145:classification; D008070:Lipopolysaccharides / Q000737:chemistry*; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D019081:O Antigens / Q000737:chemistry / Q000145:classification; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D008070:Lipopolysaccharides; D019081:O Antigens