[Effects of intralesional injection of kenalog or interferon alpha-2b on PDGF BB gene expression in situ of hypertrophic scars].
OBJECTIVE
The study is to determine whether kenalog or interferon alpha-2b inhibits fibroblast growth in hypertrophic scars through decreasing PDGF.


METHODS
Six patients with hypertrophic scars received intralesional injection of kenalog or interferon alpha-2b. On the 3rd and 7th day after injection, scar samples from each patient were collected and PDGF mRNA expressions in situ were studied.


RESULTS
1. On the 7th day after intralesional injection of kenalog or interferon alpha-2b, expressions of PDGF BB mRNAs decreased significantly (P < 0.01). However, on the 3rd day the expressions of PDGF BB mRNAs of the intralesional-injected area and non-injected area were not significantly different (P > 0.05). 2. Gene expressions of PDGF BB in situ were higher in hypertrophic scars than in normal skin.


CONCLUSIONS
The mechanism of kenalog or interferon alpha-2b in ameliorating hypertrophic scar is to reduce mitosis and prohibit proliferation of fibroblasts in hypertrophic scars by inhibiting PDGF gene expression.
Xu
S
S
Research Centre of Plastic Surgery, Third Clinical School of Beijing Medical University, Beijing 100083.
Bao
W
W
Yang
X
X
D000293:Adolescent; D000328:Adult; D000077214:Becaplermin; D002648:Child; D017439:Cicatrix, Hypertrophic / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism; D005260:Female; D005938:Glucocorticoids / Q000008:administration & dosage; D006801:Humans; D015552:Injections, Intralesional; D000077190:Interferon alpha-2; D016898:Interferon-alpha / Q000008:administration & dosage*; D008297:Male; D008875:Middle Aged; D010982:Platelet-Derived Growth Factor / Q000096:biosynthesis* / Q000235:genetics; D020574:Proto-Oncogene Proteins c-sis; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011994:Recombinant Proteins; D014222:Triamcinolone Acetonide / Q000008:administration & dosage*
D005938:Glucocorticoids; D000077190:Interferon alpha-2; D016898:Interferon-alpha; D010982:Platelet-Derived Growth Factor; D020574:Proto-Oncogene Proteins c-sis; D012333:RNA, Messenger; D011994:Recombinant Proteins; D000077214:Becaplermin; D014222:Triamcinolone Acetonide