title: Effects of apolipoprotein E genotypes and other risk factors on the development of coronary artery disease in Southern Turkey.
abstract: BACKGROUND
  Apolipoprotein E (apoE) plays a major role in lipoprotein metabolism and lipid transport. Associations between apoE genotypes, coronary artery disease (CAD) and other risk factors have been described by many investigators. The aim of this study was to investigate the role of apoE gene polymorphism and other risk factors in the development of CAD in subjects whose coronary arteries were evaluated by means of coronary angiography.
  
  
  METHODS
  The study population consisted of 199 subjects (114 male and 55 female). Of the total, 107 had CAD. The apoE gene was amplified by polymerase chain reaction (PCR) and then digested by CfoI restriction enzyme. The plasma lipid levels and other risk factors were also determined in all subjects.
  
  
  RESULTS
  The epsilon2 and epsilon4 allele frequencies and genotypes carrying epsilon4 allele were significantly higher in CAD (+) patients. Plasma lipids except triglycerides were increased in CAD (+) cases. We found that apoE genotypes, HT, DM, male gender, age and smoking were the independent predictors of CAD. There was no association between apoE alleles and lipids.
  
  
  CONCLUSIONS
  We conclude that apoE polymorphism (presence of epsilon4 allele) is associated with the development of CAD in Southern Turkey. In our study, we did not observe any effect of apoE alleles on lipid levels.
authors.affiliation: Department of Biochemistry, School of Medicine, Cukurova University, 01330, Balcali, Adana, Turkey. gattila@mail.cu.edu.tr
authors.forename: G
authors.initials: G
authors.lastname: Attila
authors.forename: E
authors.initials: E
authors.lastname: Acartürk
authors.forename: G
authors.initials: G
authors.lastname: Eskandari
authors.forename: O
authors.initials: O
authors.lastname: Akpinar
authors.forename: A
authors.initials: A
authors.lastname: Tuli
authors.forename: M
authors.initials: M
authors.lastname: KanadaşI
authors.forename: L
authors.initials: L
authors.lastname: Kayrin
mesh_terms: D000328:Adult; D000367:Age Factors; D000368:Aged; D001057:Apolipoproteins E / Q000235:genetics*; D003324:Coronary Artery Disease / Q000235:genetics*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D012044:Regression Analysis; D012307:Risk Factors; D012907:Smoking; D014421:Turkey
chemical_list: D001057:Apolipoproteins E