[The use of flow cytometry for the determination of the hepatitis B virus pre-S1 envelop protein binding by human peripheral blood lymphocytes in vitro in the course of recovery].
The course of hepatitis B virus (HBV) infection depends on the host immune response to various antigens of the virus, including hepatitis B surface antigen (HbsAg), which is present on the envelope of HBV and which contains Pre-S1, Pre-S2 and S proteins. The peripheral blood lymphocytes (PBL) from patients with chronic hepatitis B infection (CHB) and patients acutely infected with HBV and recovered completely (convalescents) were studied for detecting of Pre-S1 antigen binding. We used a cytometric method for measurement of the binding of fluorescein labeled Pre-S1 antigen (FITC/Pre-S1) to the PBL. The binding of FITC/Pre-S1 was determined on resting lymphocytes and on lymphocytes cultured in vitro for 5 days in the presence of Pre-S1 and phytohaemagglutinin-P (PHA-P). The expression of CD3 and CD19 molecules on the surface of PBLs simultaneously with the expression of FITC/Pre-S1 was also analysed using flow cytometry. We found that the Pre-S1 binding significantly depends on the state of the activation of lymphocytes. Specific stimulation of the convalescent lymphocytes with Pre-S1 resulted in an increase in the percentage of the FITC/Pre-S1 binding cells in relation to the unstimulated cultured and resting cells as well as to the cells from CHB patients. We suggest that flow cytometric measurement of Pre-S1 binding by lymphocytes may be useful indicator of the disease activity.
Sułowska
Z
Z
Centrum Mikrobiologii i Wirusologii PAN w Łodzi.
Dworniak
D
D
Tchórzewski
H
H
D003289:Convalescence; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D006514:Hepatitis B Surface Antigens / Q000097:blood / Q000302:isolation & purification* / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D011485:Protein Binding; D011498:Protein Precursors / Q000097:blood / Q000302:isolation & purification* / Q000378:metabolism*; D013601:T-Lymphocytes / Q000378:metabolism*
D006514:Hepatitis B Surface Antigens; D011498:Protein Precursors; C050806:presurface protein 1, hepatitis B surface antigen