title: Pim-1 translocates sorting nexin 6/TRAF4-associated factor 2 from cytoplasm to nucleus.
abstract: Pim-1, an oncogene product of serine/threonine kinase, has been found to play roles in apoptosis induction/suppression, cell-cycle progression and transcriptional regulation by phosphorylating the target proteins involved in these processes. The target proteins phosphorylated by Pim-1, including p100, Cdc25A, PAP-1 and heterochromatin protein 1, have been identified. The precise functions of Pim-1, however, are still poorly understood. In this study, we identified tumor necrosis factor receptor-associated factor 4-associated factor 2/sorting nexin 6 (TFAF2/SNX6) as a Pim-1-binding protein, and we found that TFAF2/SNX6 was phosphorylated and translocated from the cytoplasm to nucleus by Pim-1. This translocation of the protein was not affected by Pim-1-dependent phosphorylation. Since sorting nexins, including TFAF2/SNX6, have been reported to be located in the cytoplasm or membrane by association with several receptors of tyrosine- or serine/threonine-kinase, this is the first report of TFAF2/SNX6 being located in the nucleus after binding to Pim-1.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Ishibashi
authors.forename: H
authors.initials: H
authors.lastname: Maita
authors.forename: M
authors.initials: M
authors.lastname: Yano
authors.forename: N
authors.initials: N
authors.lastname: Koike
authors.forename: K
authors.initials: K
authors.lastname: Tamai
authors.forename: H
authors.initials: H
authors.lastname: Ariga
authors.forename: S M
authors.initials: SM
authors.lastname: Iguchi-Ariga
mesh_terms: D002352:Carrier Proteins / Q000378:metabolism*; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D000073718:Pancreatitis-Associated Proteins; D017346:Protein Serine-Threonine Kinases / Q000502:physiology*; D021381:Protein Transport; D011506:Proteins / Q000378:metabolism*; D011518:Proto-Oncogene Proteins / Q000502:physiology*; D051573:Proto-Oncogene Proteins c-pim-1; D053449:TNF Receptor-Associated Factor 4; D047988:Tumor Necrosis Factor Receptor-Associated Peptides and Proteins; D033921:Vesicular Transport Proteins
chemical_list: D002352:Carrier Proteins; D000073718:Pancreatitis-Associated Proteins; D011506:Proteins; D011518:Proto-Oncogene Proteins; C000612141:REG3A protein, human; D053449:TNF Receptor-Associated Factor 4; C506367:TRAF4 protein, human; D047988:Tumor Necrosis Factor Receptor-Associated Peptides and Proteins; D033921:Vesicular Transport Proteins; C496634:PIM1 protein, human; D017346:Protein Serine-Threonine Kinases; D051573:Proto-Oncogene Proteins c-pim-1