title: The prevalence of Chlamydia trachomatis in fresh tissue specimens from patients with ectopic pregnancy or tubal factor infertility as determined by PCR and in-situ hybridisation.
abstract: The prevalence of chlamydial DNA determined by PCR and in-situ hybridisation (ISH) in fresh tissue specimens (endometrium, fallopian tube and ovary) was investigated in 33 women presenting with ectopic pregnancy (EP), 14 women with tubal factor infertility (TFI) and 50 control patients from the UK and the West Indies. In the UK EP group, chlamydial DNA was detected by PCR in 56% of patients; similar results were found in the Trinidad EP group (67%). In the TFI group, chlamydial DNA was detected in (71%) of patients by PCR. The detection of Chlamydia trachomatis DNA by ISH was highest in the TFI group (43%). Women presenting with EP and TFI showed evidence of previous or current genital C. trachomatis infection, underlining the importance of this microorganism in the development of these conditions. Importantly, chlamydial DNA could be detected in DNA preparations from the endometrium, fallopian tube and ovary of EP and TFI patients at the time of surgery.
authors.forename: Rachael E L
authors.initials: REL
authors.lastname: Barlow
authors.affiliation: Division of Genomic Medicine, University of Sheffield Medical School, Floor F, Sheffield S10 2RX, *Department of Obstetrics and Gynaecology, Divison of Surgical and Anaesthetic Sciences, University of Sheffield Medical School, Sheffield, †Department of Pathology, Royal Liverpool and Broadgreen University Hospitals NHS Trust, Liverpool, ‡Division of Obstetrics and Gynaecology, Department of Hospital Medicine, University of Bristol, Bristol, UK and §Department of Clinical Surgical Sciences, University of the West Indies, St Augustine, Trinidad.
authors.forename: Ian D
authors.initials: ID
authors.lastname: Cooke
authors.affiliation: Division of Genomic Medicine, University of Sheffield Medical School, Floor F, Sheffield S10 2RX, *Department of Obstetrics and Gynaecology, Divison of Surgical and Anaesthetic Sciences, University of Sheffield Medical School, Sheffield, †Department of Pathology, Royal Liverpool and Broadgreen University Hospitals NHS Trust, Liverpool, ‡Division of Obstetrics and Gynaecology, Department of Hospital Medicine, University of Bristol, Bristol, UK and §Department of Clinical Surgical Sciences, University of the West Indies, St Augustine, Trinidad.
authors.forename: Olusegun
authors.initials: O
authors.lastname: Odukoya
authors.affiliation: Division of Genomic Medicine, University of Sheffield Medical School, Floor F, Sheffield S10 2RX, *Department of Obstetrics and Gynaecology, Divison of Surgical and Anaesthetic Sciences, University of Sheffield Medical School, Sheffield, †Department of Pathology, Royal Liverpool and Broadgreen University Hospitals NHS Trust, Liverpool, ‡Division of Obstetrics and Gynaecology, Department of Hospital Medicine, University of Bristol, Bristol, UK and §Department of Clinical Surgical Sciences, University of the West Indies, St Augustine, Trinidad.
authors.forename: Mark K
authors.initials: MK
authors.lastname: Heatley
authors.affiliation: Division of Genomic Medicine, University of Sheffield Medical School, Floor F, Sheffield S10 2RX, *Department of Obstetrics and Gynaecology, Divison of Surgical and Anaesthetic Sciences, University of Sheffield Medical School, Sheffield, †Department of Pathology, Royal Liverpool and Broadgreen University Hospitals NHS Trust, Liverpool, ‡Division of Obstetrics and Gynaecology, Department of Hospital Medicine, University of Bristol, Bristol, UK and §Department of Clinical Surgical Sciences, University of the West Indies, St Augustine, Trinidad.
authors.forename: Julian
authors.initials: J
authors.lastname: Jenkins
authors.affiliation: Division of Genomic Medicine, University of Sheffield Medical School, Floor F, Sheffield S10 2RX, *Department of Obstetrics and Gynaecology, Divison of Surgical and Anaesthetic Sciences, University of Sheffield Medical School, Sheffield, †Department of Pathology, Royal Liverpool and Broadgreen University Hospitals NHS Trust, Liverpool, ‡Division of Obstetrics and Gynaecology, Department of Hospital Medicine, University of Bristol, Bristol, UK and §Department of Clinical Surgical Sciences, University of the West Indies, St Augustine, Trinidad.
authors.forename: Gordon
authors.initials: G
authors.lastname: Narayansingh
authors.affiliation: Division of Genomic Medicine, University of Sheffield Medical School, Floor F, Sheffield S10 2RX, *Department of Obstetrics and Gynaecology, Divison of Surgical and Anaesthetic Sciences, University of Sheffield Medical School, Sheffield, †Department of Pathology, Royal Liverpool and Broadgreen University Hospitals NHS Trust, Liverpool, ‡Division of Obstetrics and Gynaecology, Department of Hospital Medicine, University of Bristol, Bristol, UK and §Department of Clinical Surgical Sciences, University of the West Indies, St Augustine, Trinidad.
authors.forename: Samuel S
authors.initials: SS
authors.lastname: Ramsewak
authors.forename: Adrian
authors.initials: A
authors.lastname: Eley
mesh_terms: D000328:Adult; D002690:Chlamydia Infections / Q000453:epidemiology / Q000382:microbiology*; D002692:Chlamydia trachomatis / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D005184:Fallopian Tube Diseases / Q000150:complications / Q000382:microbiology*; D005260:Female; D005836:Genitalia, Female / Q000382:microbiology*; D006801:Humans; D017403:In Situ Hybridization; D007247:Infertility, Female / Q000453:epidemiology / Q000382:microbiology*; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D011271:Pregnancy, Ectopic / Q000453:epidemiology / Q000382:microbiology*; D015995:Prevalence; D014300:Trinidad and Tobago / Q000453:epidemiology; D006113:United Kingdom / Q000453:epidemiology
chemical_list: D004269:DNA, Bacterial