Colocalization of neuronal nitric oxide synthase and neurokinin-1 receptor in striatal interneurons in the rat.
It has been established that nitric oxide synthase (NOS)-containing aspiny neurons constitute one class of interneurons in the striatum, and that substance P (SP)-containing projection neurons give off many axon collaterals within the striatum. In the present study, we investigated a morphological substrate of possible influences of SP-containing projection neurons upon NOS-containing interneuron in the rat striatum; colocalization of immunoreactivities for NOS and NK-1 type tachykinin receptor (NK1R: SP receptor) was examined by a double-immunofluorescence histochemistry. The vast majority (94.6%) of NOS-positive neurons showed NK1R immunoreactivity, whereas only smaller cells of NK1R-positive neurons (61.2% of NK1R-positive neurons) displayed NOS immunoreactivity. The results indicated that the NOS-containing interneurons were under direct control of SP-containing projection neurons in the striatum.
Li
J L
JL
Department of Anatomy and K. K. Leung Brain Research Center, The Fourth Military Medical University, Xi'an 710032, People's Republic of China.
Kaneko
T
T
Mizuno
N
N
D000818:Animals; D003342:Corpus Striatum / Q000166:cytology*; D005455:Fluorescent Antibody Technique; D007395:Interneurons / Q000737:chemistry* / Q000201:enzymology*; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000032:analysis*; D051381:Rats; D017208:Rats, Wistar; D018040:Receptors, Neurokinin-1 / Q000032:analysis*; D013373:Substance P / Q000378:metabolism
D018040:Receptors, Neurokinin-1; D009569:Nitric Oxide; D013373:Substance P; D019001:Nitric Oxide Synthase