title: Cloning and functional expression of ASIC-beta2, a splice variant of ASIC-beta.
abstract: We have isolated a cDNA encoding a splice variant of ASIC (acid-sensing ion channel)-beta from the rat trigeminal ganglion. This clone, designated ASIC-beta2, showed a 342 base deletion just after the first transmembrane domain in ASIC-beta. RT-PCR experiments revealed that ASIC-beta2 was expressed exclusively in the trigeminal ganglion and dorsal root ganglion. In situ hybridization showed that ASIC-beta2 mRNA was concentrated in both small diameter and large diameter neurons and co-localized with ASIC-beta mRNA within single sensory neurons in the trigeminal ganglion. When expressed in Xenopus oocytes, ASIC-beta2 was inactive by itself. However, it associated with ASIC-beta to form heteromers, which display lower affinity for protons than ASIC-beta alone.
authors.affiliation: Department of Anatomy II, Nagoya City University Medical School, 1 Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya 467-8601, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Ugawa
authors.forename: T
authors.initials: T
authors.lastname: Ueda
authors.forename: E
authors.initials: E
authors.lastname: Takahashi
authors.forename: Y
authors.initials: Y
authors.lastname: Hirabayashi
authors.forename: T
authors.initials: T
authors.lastname: Yoneda
authors.forename: S
authors.initials: S
authors.lastname: Komai
authors.forename: S
authors.initials: S
authors.lastname: Shimada
mesh_terms: D062565:Acid Sensing Ion Channels; D017398:Alternative Splicing / Q000502:physiology*; D000595:Amino Acid Sequence / Q000502:physiology; D000818:Animals; D001483:Base Sequence / Q000502:physiology; D048429:Cell Size / Q000235:genetics; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000302:isolation & purification*; D017950:Ganglia, Sensory / Q000166:cytology / Q000378:metabolism*; D015870:Gene Expression / Q000502:physiology*; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins; D009865:Oocytes / Q000378:metabolism; D010146:Pain / Q000378:metabolism* / Q000503:physiopathology; D020033:Protein Isoforms / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015222:Sodium Channels / Q000235:genetics*; D012668:Trigeminal Ganglion / Q000166:cytology / Q000378:metabolism*; D014982:Xenopus laevis
chemical_list: C112231:ASIC3 protein, human; D062565:Acid Sensing Ion Channels; D018076:DNA, Complementary; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D020033:Protein Isoforms; D012333:RNA, Messenger; D015222:Sodium Channels