Protein kinase C gamma-like immunoreactivity in the substantia gelatinosa of the medullary dorsal horn of the rat.
We examined protein kinase C gamma-immunoreactivity (PKCgamma-IR) in the substantia gelatinosa (SG) of the rat medullary dorsal horn (MDH). The density of PKCgamma-IR in the MDH was most intense in the SG. The number of neurons with PKCgamma-IR were also much larger in the SG than in the other layers of the MDH. Double-immunohistochemical studies indicated light and electron microscopically that substance P-containing fibers and I-B4 (isolectin from Bandeiraea simplicifolia)-labeled fibers made synapses on SG neurons with PKCgamma-IR, indicating that SG neurons with PKCgamma might receive nociceptive primary afferent fibers. The results support the notion that PKCgamma in the MDH may contribute to the regulation of the nociception.
Li
J L
JL
Department of Anatomy and K. K. Leung Brain Research Centre, The Fourth Military Medical University, Xi'an 710032, People's Republic of China.
Li
Y Q
YQ
Nomura
S
S
Kaneko
T
T
Mizuno
N
N
D000344:Afferent Pathways / Q000201:enzymology* / Q000648:ultrastructure; D000818:Animals; D002451:Cell Compartmentation / Q000502:physiology; D048429:Cell Size / Q000502:physiology; D003712:Dendrites / Q000201:enzymology / Q000648:ultrastructure; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000378:metabolism*; D037102:Lectins / Q000378:metabolism / Q000493:pharmacokinetics*; D008297:Male; D008854:Microscopy, Electron; D009619:Nociceptors / Q000201:enzymology* / Q000648:ultrastructure; D010146:Pain / Q000473:pathology / Q000503:physiopathology; D017729:Presynaptic Terminals / Q000201:enzymology* / Q000648:ultrastructure; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013126:Spinal Nerve Roots / Q000201:enzymology* / Q000648:ultrastructure; D013373:Substance P / Q000378:metabolism; D013376:Substantia Gelatinosa / Q000201:enzymology* / Q000648:ultrastructure; D009435:Synaptic Transmission / Q000502:physiology; D014275:Trigeminal Caudal Nucleus / Q000201:enzymology* / Q000648:ultrastructure
D007527:Isoenzymes; D037102:Lectins; D013373:Substance P; C087857:protein kinase C gamma; D011493:Protein Kinase C