title: MMP-19: cellular localization of a novel metalloproteinase within normal breast tissue and mammary gland tumours.
abstract: Matrix metalloproteinases (MMPs) are instrumental in promoting and facilitating the spread of malignant diseases and in the de novo formation of blood vessels. This study has mapped the immunoreactivity of a novel, angiogenesis-related metalloproteinase--MMP-19--in normal breast tissue and in benign and malignant breast lesions and compared this pattern of expression with that of MMP-2. In the normal resting mammary gland, MMP-19 was strongly expressed in the myoepithelial layer of the ductal system; the alveolar and ductal epithelia displayed considerable, but lobule-specific, variations in labelling intensity. MMP-19 was also present within the smooth muscle and endothelial layers of large and medium-sized blood vessels, as well as within capillary walls. In benign lesions, all tumour cells and their surrounding vasculature were uniformly and strongly immunoreactive for MMP-19. Progression towards an invasive phenotype and neoplastic dedifferentiation led to the disappearance of MMP-19 from tumour cells and blood vessels and a concomitant rise in the levels of MMP-2. In vitro experiments conducted with isolated smooth muscle cells cultivated on a solid substratum, or within the interstices of a collagen matrix, indicated that the expression of MMP-19 is influenced by the architecture of the surrounding extracellular matrix.
authors.affiliation: Institute of Anatomy, University of Bern, Switzerland.
authors.forename: V
authors.initials: V
authors.lastname: Djonov
authors.forename: K
authors.initials: K
authors.lastname: Högger
authors.forename: R
authors.initials: R
authors.lastname: Sedlacek
authors.forename: J
authors.initials: J
authors.lastname: Laissue
authors.forename: A
authors.initials: A
authors.lastname: Draeger
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001940:Breast / Q000201:enzymology*; D001943:Breast Neoplasms / Q000201:enzymology* / Q000473:pathology; D018929:Cell Culture Techniques; D018450:Disease Progression; D015536:Down-Regulation; D004730:Endothelium, Vascular / Q000201:enzymology; D004847:Epithelial Cells / Q000201:enzymology; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D053505:Matrix Metalloproteinases, Secreted; D008666:Metalloendopeptidases / Q000378:metabolism*; D008875:Middle Aged; D009130:Muscle, Smooth / Q000201:enzymology; D009361:Neoplasm Invasiveness
chemical_list: D053505:Matrix Metalloproteinases, Secreted; D008666:Metalloendopeptidases; C108362:matrix metalloproteinase 19; D020778:Matrix Metalloproteinase 2