Natronobacterium nitratireducens sp. nov., a aloalkaliphilic archaeon isolated from a soda lake in China.
Two novel haloalkaliphilic archaea, strains C231T and C42, were isolated from a soda lake in China. Cells of the two strains were rod-shaped and gram-negative and colonies were bright red. They required at least 2.5 M NaCl for growth, with an optimum at 3.5 M NaCl, and grew over a pH range from 8.0 to 10.5, with an optimum at pH 8.5. Hypotonic treatment with less than 1.5 M NaCl caused cell lysis. They had similar polar lipid compositions, possessing the diphytanyl (C20:C20) and phytanyl-sesterterpanyl (C20:C25) diether derivatives of phosphatidylglycerol and phosphatidylglycerophosphate methyl ester and a minor phospholipid, PL1. No glycolipids were detected. Comparison of 16S rDNA sequences and morphological features placed them in the genus Natronobacterium. Detailed phenotypic characterization and DNA-DNA hybridization studies revealed that the two strains belong to a new species in the genus Natronobacterium, for which the name Natronobacterium nitratireducens sp. nov. is proposed. The type strain is C231T (= AS 1.1980T = JCM 10879T).
Xin
H
H
Itoh
T
T
Zhou
P
P
Suzuki
K
K
Nakase
T
T
D002681:China; D004275:DNA, Ribosomal / Q000032:analysis; D005618:Fresh Water / Q000382:microbiology*; D008969:Molecular Sequence Data; D019633:Natronobacterium / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride
D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride