Ellagic acid formation from galloylglucoses by a crude enzyme of Cornus capitata adventitious roots.
The aqueous extract of acetone powder, which had been prepared from Cornus capitata 'Mountain Moon' adventitious roots, cultured in MS medium with a high concentration of Cu2+(10 microM), showed strong oxidative activity toward galloylglucoses. A compound formed from galloyglucoses, such as 1,2,3,4,6-penta-O-galloyl-beta-D-glucose and tannic acid, by the reaction with the crude enzyme solution of the adventitious roots was isolated and characterized as ellagic acid by spectrometric analyses.
Tanaka
N
N
Department of Applied Biological Sciences, Faculty of Agriculture, Saga University, Japan. norie@cc.saga-u.ac.jp
Shimomura
K
K
Ishimaru
K
K
D002851:Chromatography, High Pressure Liquid; D029767:Cornaceae / Q000737:chemistry*; D003470:Culture Media; D004610:Ellagic Acid / Q000138:chemical synthesis*; D005707:Gallic Acid / Q000737:chemistry; D047348:Hydrolyzable Tannins; D009682:Magnetic Resonance Spectroscopy; D010936:Plant Extracts / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D013634:Tannins / Q000737:chemistry*
D003470:Culture Media; D047348:Hydrolyzable Tannins; D010936:Plant Extracts; D013634:Tannins; D004610:Ellagic Acid; C060474:glucogallin; D005707:Gallic Acid