Application of analytical and preparative high-speed counter-current chromatography for separation of lycopene from crude extract of tomato paste.
Lycopene was isolated from 100 mg of crude extract of tomato paste containing about 9% of lycopene. Analytical high-speed counter-current chromatography (HSCCC) was first used for the systematic selection of the two-phase solvent system. Then preparative HSCCC separation was performed with a nonaqueous solvent system composed of n-hexane-dichloromethane-acetonitrile at an optimum volume ratio of 10:3.5:6.5. This yielded 8.6 mg of lycopene at over 98.5% purity as determined by HPLC analysis.
Wei
Y
Y
Beijing Research Center for Separation and Purification Technologies of Natural Prodiucts, Beijing Institute of New Technology Application, PR China.
Zhang
T
T
Xu
G
G
Ito
Y
Y
D002338:Carotenoids / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D003377:Countercurrent Distribution / Q000379:methods*; D000077276:Lycopene; D018551:Solanum lycopersicum / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D010936:Plant Extracts / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
D010936:Plant Extracts; D002338:Carotenoids; D000077276:Lycopene