[Is there a relation between Alzheimer s disease and defects of mitochondrial DNA?].
BACKGROUND
Various studies have related Alzheimer s disease (AD) with mitochrondrial defects. These defects may be structural, biochemical or genetic in type. Amongst the genetic defects the rearrangement and particular mutations described in mitochondrial DNA (mtDNA) are striking.


OBJECTIVE
To study the incidence of rearrangement and 4 particular mutations in the mtDNA of patients with AD, and determine the possible differences from persons taken as controls.


METHODS
Necropsies of the cerebellum, frontal cortex and hippocampus of patients with AD and controls. We also used blood from living patients diagnosed as having EA and from controls. The samples were analysed using hybrid Southern blot with a mitochondrial probe. Particular mutations G3196A, A3397G, A4336G and G5460A/T were also analysed.


RESULTS
No differences were found between the patients and controls, in either brain tissue or blood on analysis using Southern. No association was found between the particular mutations analysed and the AD of our samples.


CONCLUSIONS
The results obtained did not support the hypothesis of involvement of mitochondria in AD. Regarding rearrangement and the 4 particular mutations analysed in the mtDNA of our samples. However, this does not rule out the possible existence of other particular mutations which were not analysed and/or other mitochondrial defects which contribute to the development of AD.
Rodríguez Santiago
B
B
Hospital Duran i Reynals. Centro de Genética Médica y Molecular-IRO, L'Hospitalet de Llobregat, 08907, España.
Casademont
J
J
Nunes
V
V
D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000235:genetics*; D001921:Brain / Q000473:pathology; D004272:DNA, Mitochondrial / Q000235:genetics*; D004579:Electron Transport / Q000235:genetics; D017353:Gene Deletion; D006801:Humans; D008875:Middle Aged; D016874:Neurofibrillary Tangles / Q000473:pathology; D017354:Point Mutation / Q000235:genetics; D016133:Polymerase Chain Reaction
D004272:DNA, Mitochondrial