A proton NMR study of the effect of a new intravasal injectable male contraceptive RISUG on seminal plasma metabolites.
Nuclear magnetic resonance (NMR) spectroscopy was used to quantify citrate, glucose, lactate, glycerophosphorylcholine and choline in seminal plasma from subjects injected with a new male contraceptive RISUG, a copolymer of styrene maleic anhydride dissolved in dimethyl sulphoxide, and in seminal plasma from normal ejaculates. No significant difference in the concentration of citrate was observed between the groups, indicating that the prostate is not affected by the contraceptive. The concentrations of glucose, lactate, glycerophosphorylcholine and choline were significantly lower (P < 0.01) in subjects injected with RISUG compared with controls. In addition, metabolite ratios such as choline:citrate, citrate:lactate, choline:lactate and glycerophosphorylcholine:choline were calculated. Citrate:lactate and glycerophosphorylcholine:choline ratios were significantly lower in RISUG-injected subjects than in controls (P < 0.01), thereby indicating the occurrence of partial obstructive azoospermia. The most important finding of the present study was that the intervention of RISUG in the vas deferens even for a period as long as 8 years is absolutely safe and does not lead to prostatic diseases.
Sharma
U
U
Department of NMR, All India Institute of Medical Sciences, New Delhi 110 029, India.
Chaudhury
K
K
Jagannathan
N R
NR
Guha
S K
SK
D000328:Adult; D002794:Choline / Q000032:analysis / Q000378:metabolism; D019343:Citric Acid / Q000032:analysis / Q000378:metabolism; D003272:Contraceptive Agents, Male / Q000008:administration & dosage / Q000494:pharmacology*; D004121:Dimethyl Sulfoxide / Q000008:administration & dosage / Q000494:pharmacology*; D005947:Glucose / Q000032:analysis / Q000378:metabolism; D005997:Glycerylphosphorylcholine / Q000032:analysis / Q000378:metabolism; D006801:Humans; D007267:Injections; D019344:Lactic Acid / Q000032:analysis / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D008298:Maleates / Q000008:administration & dosage / Q000494:pharmacology*; D011091:Polyesters; D011137:Polystyrenes; D011467:Prostate / Q000187:drug effects / Q000502:physiology; D012661:Semen / Q000378:metabolism*; D013343:Styrenes / Q000008:administration & dosage / Q000494:pharmacology*
D003272:Contraceptive Agents, Male; D008298:Maleates; D011091:Polyesters; D011137:Polystyrenes; D013343:Styrenes; C440495:contraceptive agent, RISUG; D019343:Citric Acid; D019344:Lactic Acid; D005997:Glycerylphosphorylcholine; D005947:Glucose; D002794:Choline; D004121:Dimethyl Sulfoxide