High-performance liquid chromatographic assay for the determination of 2'-deoxy-3'-thiacytidine (lamivudine) in human plasma.
A method for the quantification of 2'-deoxy-3'-thiacytidine (lamivudine, 3-TC), which incorporated the use of 3-isobutyl-methylxanthine as internal standard (I.S.) was developed and validated in human plasma, using HPLC with UV absorbance detection. Using solid-phase extraction, 3-TC and I.S. were selectively extracted from human plasma. Subsequently, chromatographic separation was performed using a YMC phenyl column with ion-pair chromatography and detection at 270 nm. The method was validated over a concentration range of 10 to 5,000 ng/ml using 0.5 ml of human plasma. The extraction recovery for both 3-TC and I.S. was greater than 95%. The determination of inter- and intra-day precision (RSD) was less than 10% at all concentration levels, while the inter- and intra-day accuracy (% difference) was less than 6%.
Zheng
J J
JJ
Drug Metabolism and Pharmacokinetic Section, The DuPont Pharmaceutical Company, Newark, DE 19714, USA. joanna.j.zheng@dupontpharma.com
Wu
S T
ST
Emm
T A
TA
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D019259:Lamivudine / Q000097:blood*; D012015:Reference Standards; D015203:Reproducibility of Results; D018894:Reverse Transcriptase Inhibitors / Q000097:blood*; D012680:Sensitivity and Specificity
D018894:Reverse Transcriptase Inhibitors; D019259:Lamivudine