title: Disappearance of clonally expanded T cells after allogeneic leukocyte immunotherapy in peripheral blood of patients with habitual abortion.
abstract: Allogeneic leukocyte immunotherapy is often used to improve fertility of patients with habitual abortion (HA), which probably acts through immune-mediated mechanisms. However, the involvement of T cells is not clear. This study examined the effect of allogeneic immunotherapy on T cells of patients with HA. Peripheral blood mononuclear cells were obtained from 5 healthy women and 14 women with HA. RNA was isolated from mononuclear blood cells. Reverse transcription-polymerase chain reaction (RT-PCR), followed by single-strand conformational polymorphism (SSCP) were used to analyze the gene segments of T-cell receptor beta chain (TCRbetaV) variable regions. Oligoclonal accumulation of T cells was identified in peripheral blood of nonpregnant patients with a history of HA. It was also revealed, however, that immunostimulation reduced the number of accumulating T-cell clones (p = 0.0004). The results, together with the clinical effectiveness of immunotherapy, suggest that accumulation of T-cell clonotypes, which probably resulted from antigenic stimulation, is involved in the pathogenesis of HA.
authors.affiliation: Rheumatology, Immunology and Genetics Program, Institute of Medical Science, Kanagawa, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Masuko-Hongo
authors.forename: K
authors.initials: K
authors.lastname: Hayashi
authors.forename: K
authors.initials: K
authors.lastname: Yonamine
authors.forename: M
authors.initials: M
authors.lastname: Tokuyama
authors.forename: K
authors.initials: K
authors.lastname: Nishioka
authors.forename: T
authors.initials: T
authors.lastname: Kato
mesh_terms: D000026:Abortion, Habitual / Q000097:blood / Q000276:immunology* / Q000628:therapy*; D019264:Adoptive Transfer; D000328:Adult; D000595:Amino Acid Sequence; D002999:Clone Cells; D005260:Female; D006801:Humans; D007279:Injections, Subcutaneous; D008213:Lymphocyte Activation; D017710:Lymphocyte Transfusion; D008297:Male; D008969:Molecular Sequence Data; D018807:Polymorphism, Single-Stranded Conformational; D011247:Pregnancy; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism
chemical_list: D016693:Receptors, Antigen, T-Cell, alpha-beta