[Endogenous substance P in corneal epithelial cells and keratocytes].
OBJECTIVE
To detect endogenous substance P(SP) and neurokinin receptor 1(NK1R) in cultured human epithelial cells of the cornea(HE) and human keratocytes(HK).


METHODS
Messenger RNA(mRNA) expression of SP and endogenous SP in HE and HK were detected by reverse transcription-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA). mRNA expression of NK1R in HE and HK was detected by RT-PCR.


RESULTS
The mRNA expression of SP and endogenous SP were recognized in HE and HK. The mRNA of NK1R was also expressed in HE and HK.


CONCLUSIONS
It appears that endogenous SP regulates the biological functions of HE and HK in an autocrine or paracrine fashion.
Watanabe
M
M
Eye Department, Asama Municipal Hospital.
Nakayasu
K
K
Iwatsu
M
M
Kanai
A
A
D000368:Aged; D002478:Cells, Cultured; D019573:Epithelium, Corneal / Q000737:chemistry* / Q000166:cytology; D005260:Female; D006801:Humans; D015603:Keratinocytes / Q000737:chemistry*; D008297:Male; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D018040:Receptors, Neurokinin-1 / Q000032:analysis; D013373:Substance P / Q000032:analysis*
D012333:RNA, Messenger; D018040:Receptors, Neurokinin-1; D013373:Substance P