Enhancement of human osteoblast proliferation and phenotypic expression when cultured in human serum.
Traditionally, culture medium is supplemented with foetal bovine serum (FBS). However, in cultures of osteoblasts intended for human re-implantation, such serum presents potential risks of foreign protein contamination and transmission of viral or prion-related material, if used. We cultured human osteoblasts from 16 patients in 10% autologous human serum, 10% pooled human serum, 10% FBS or 2% Ultroser G. Non-synthetic sera were tested in both heat-treated and non-heat-treated forms. We determined cell growth and osteoblast phenotype. Cell proliferation in all types of human serum was significantly greater than in FBS. This was most marked in heat-treated autologous human serum. Cells cultured in Ultroser G had less proliferation than all other groups. The phenotypic tests showed that cells cultured in human and foetal bovine serum displayed an osteoblast phenotype, with greater protein expression in cells cultured in human serum. We conclude that culture of human osteoblasts in autologous human serum enhances cell proliferation, while maintaining an osteoblast phenotype. These findings have implications for the use of cultured osteoblasts in self-cell therapy. Human osteoblast growth is supported by autologous human serum, which allows re-implantation of cultured cells, while avoiding the risk of foreign protein carry-over with enhancement of cell proliferation.
Hankey
D P
DP
Department of Anatomy, School of Clinical Medicine, The Queen's University of Belfast, Medical Biology Centre, UK.
McCabe
R E
RE
Doherty
M J
MJ
Nolan
P C
PC
McAlinden
M G
MG
Nelson
J
J
Wilson
D J
DJ
D000368:Aged; D000369:Aged, 80 and over; D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D001802:Blood Substitutes / Q000494:pharmacology*; D002417:Cattle; D018929:Cell Culture Techniques / Q000379:methods*; D002455:Cell Division / Q000502:physiology*; D003470:Culture Media / Q000592:standards*; D005069:Evaluation Studies as Topic; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology*; D006801:Humans; D008297:Male; D008855:Microscopy, Electron, Scanning; D008875:Middle Aged; D009930:Organic Chemicals; D010006:Osteoblasts / Q000502:physiology* / Q000648:ultrastructure*; D015675:Osteocalcin / Q000032:analysis; D010641:Phenotype; D012709:Serum Albumin / Q000494:pharmacology*; D012710:Serum Albumin, Bovine / Q000494:pharmacology*; D014182:Transplantation, Autologous
D001802:Blood Substitutes; D003470:Culture Media; D009930:Organic Chemicals; D012709:Serum Albumin; C044609:Ultroser G; D015675:Osteocalcin; D012710:Serum Albumin, Bovine; D000469:Alkaline Phosphatase