title: Chloride secretion induced by thermostable direct haemolysin of Vibrio parahaemolyticus depends on colonic cell maturation.
abstract: Vibrio parahaemolyticus produces a thermostable direct haemolysin (TDH) that has been implicated in the pathogenesis of diarrhoeal disease caused by this organism. In previous work, TDH induced Cl- secretion in human colonic epithelial cells that was dependent on the intracellular Ca2+ concentration, [Ca2+]in. This study investigated whether Cl- secretion induced by TDH is influenced by the stage of maturation of intestinal epithelial cells. Two different human colonic cell lines, villus cell-like Caco-2 cells and crypt cell-like T84 cells, cultured by different methods to obtain differentiated samples, were used. When these cells were exposed to butyrate, a transcriptional regulator of differentiation genes, or co-cultured with 18Co cells, a human colonic fibroblast cell line, they showed increased trans-epithelial resistance and villus cell marker enzyme activity. In Caco-2 cells, exposure to butyrate or co-culturing with 18Co cells resulted in increased TDH binding, higher short-circuit currents (Isc) and greater [Ca2+]in. These results suggest that sensitivity to TDH is affected by the stage of cellular differentiation of cultured intestinal epithelial cells.
authors.affiliation: Department of Bacterial Infections, Research Institute for Microbial Diseases, Osaka University, 3-1, Yamadaoka, Suita, Osaka 565-0871 and *Department of Nutrition, School of Medicine, Tokushima University, Kuramoto-cho, 3-15-18, Tokushima 770-8503, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Takahashi
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Iida
authors.forename: Rochman
authors.initials: R
authors.lastname: Naim
authors.affiliation: Department of Bacterial Infections, Research Institute for Microbial Diseases, Osaka University, 3-1, Yamadaoka, Suita, Osaka 565-0871 and *Department of Nutrition, School of Medicine, Tokushima University, Kuramoto-cho, 3-15-18, Tokushima 770-8503, Japan.
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Naykaya
authors.forename: Takeshi
authors.initials: T
authors.lastname: Honda
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D001426:Bacterial Proteins; D002087:Butyrates / Q000494:pharmacology; D018938:Caco-2 Cells; D002118:Calcium / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D018118:Chloride Channels / Q000037:antagonists & inhibitors; D002712:Chlorides / Q000502:physiology*; D018920:Coculture Techniques; D003106:Colon / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D006460:Hemolysin Proteins / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D007413:Intestinal Mucosa / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D018408:Patch-Clamp Techniques; D013393:Sucrase / Q000378:metabolism; D014735:Vibrio Infections / Q000473:pathology*; D014736:Vibrio parahaemolyticus / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D002087:Butyrates; D018118:Chloride Channels; D002712:Chlorides; D006460:Hemolysin Proteins; C073845:hemolysin, Vibrio; D000469:Alkaline Phosphatase; D013393:Sucrase; D002118:Calcium