title: Insulin-like growth factor type 1 upregulates uncoupling protein 3.
abstract: In this study the expression of uncoupling protein 3 (UCP3) and its regulation by insulin-like growth factor 1 (IGF-I) and insulin in human neuroblastoma SH-SY5Y cells were characterized. Reverse transcriptase-PCR, Western blot, and immunofluorescence analysis showed that SH-SY5Y cells express UCP3 natively. IGF-I induced a time- and concentration-dependent induction of UCP3 protein reaching a twofold expression after 72 h with 10 nM IGF-I. Extremely high insulin concentrations (860 nM) and 10 nM trIGF-I, a truncated form of IGF-I with the same affinity for the IGF-I receptor as the full-length IGF-I, but with lower activity on the insulin receptor, also upregulated UCP3. We conclude that SH-SY5Y cells express UCP3 natively and that the expression is regulated by IGF-I via the IGF-I receptor.
authors.affiliation: Department of Neurochemistry & Neurotoxicology, Stockholm University, Stockholm, SE-106 91, Sweden.
authors.forename: H
authors.initials: H
authors.lastname: Gustafsson
authors.forename: L
authors.initials: L
authors.lastname: Adamson
authors.forename: J
authors.initials: J
authors.lastname: Hedander
authors.forename: E
authors.initials: E
authors.lastname: Walum
authors.forename: A
authors.initials: A
authors.lastname: Forsby
mesh_terms: D002352:Carrier Proteins / Q000096:biosynthesis / Q000235:genetics*; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism; D003929:Diabetic Neuropathies / Q000209:etiology; D006801:Humans; D007328:Insulin / Q000494:pharmacology; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D007473:Ion Channels; D008928:Mitochondria / Q000378:metabolism; D024101:Mitochondrial Proteins; D009417:Nerve Tissue / Q000187:drug effects / Q000378:metabolism*; D009447:Neuroblastoma; D017526:Receptor, IGF Type 1 / Q000302:isolation & purification; D011972:Receptor, Insulin / Q000302:isolation & purification; D014407:Tumor Cells, Cultured; D014475:Uncoupling Agents / Q000378:metabolism; D000071247:Uncoupling Protein 3; D015854:Up-Regulation
chemical_list: D002352:Carrier Proteins; D007328:Insulin; D007473:Ion Channels; D024101:Mitochondrial Proteins; C000604392:UCP3 protein, human; D014475:Uncoupling Agents; D000071247:Uncoupling Protein 3; D007334:Insulin-Like Growth Factor I; D017526:Receptor, IGF Type 1; D011972:Receptor, Insulin