Regeneration of Cuphea tolucana Peyr. in in vitro culture.
In order to regenerate Cuphea tolucana from hypocotyl, cotyledon and root explants, a solid culture and 8 hormone combinations were used. Only the root explants did not react to any of the media. On most of the media, the other explants formed shoots, roots or callus, or their reaction was more complex. The regeneration probably occurred via direct organogenesis. The regenerants displayed a wide variety of morphological characteristics. However, their offspring did not show any differences from plants, which had not undergone culture.
Przybecki
Z
Z
Department of Plant Genetics, Breeding and Plant Biotechnology, Warsaw University of Agriculture, Nowoursynowska 166, 02-787 Warszawa, Poland.
Olejniczak
J
J
Adamska
E
E
D018929:Cell Culture Techniques / Q000379:methods*; D018548:Cotyledon / Q000187:drug effects / Q000254:growth & development; D003470:Culture Media; D005314:Embryonic and Fetal Development / Q000187:drug effects; D018546:Hypocotyl / Q000187:drug effects / Q000254:growth & development; D019684:Magnoliopsida / Q000187:drug effects / Q000254:growth & development*; D010937:Plant Growth Regulators / Q000494:pharmacology; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development; D018520:Plant Shoots / Q000187:drug effects / Q000254:growth & development; D012038:Regeneration / Q000187:drug effects
D003470:Culture Media; D010937:Plant Growth Regulators