title: Identification of Staphylococcus spp. by PCR-restriction fragment length polymorphism of gap gene.
abstract: Oligonucleotide primers specific for the Staphylococcus aureus gap gene were previously designed to identify 12 Staphylococcus spp. by PCR. In the present study, AluI digestion of PCR-generated products rendered distinctive restriction fragment length polymorphism patterns that allowed 24 Staphylococcus spp. to be identified with high specificity.
authors.affiliation: Departamento de Sanidad Animal, Microbiología e Inmunología, Universidad de León, 24071 León, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Yugueros
authors.forename: A
authors.initials: A
authors.lastname: Temprano
authors.forename: M
authors.initials: M
authors.lastname: Sánchez
authors.forename: J M
authors.initials: JM
authors.lastname: Luengo
authors.forename: G
authors.initials: G
authors.lastname: Naharro
mesh_terms: D000818:Animals; D004269:DNA, Bacterial / Q000032:analysis; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D013045:Species Specificity; D013211:Staphylococcus aureus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D004269:DNA, Bacterial; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; C024669:endodeoxyribonuclease AluI; D015252:Deoxyribonucleases, Type II Site-Specific