Ligand-histone H1 conjugates: increased solubility of DNA complexes, but no enhanced transfection activity.
We introduced galactose and a short RGD sequence as ligands into H1 histone to target the asialoglycoprotein receptor or integrins on cells expressing these receptors. The efficiency of the gene transfer mediated by galactosylated H1 histone was strongly affected by the transfection conditions. Galactosylation of H1 led to an increase of the basic H1-mediated gene transfer activity only, when H1 itself did not develop its optimal transfection activity. Under other conditions any specific gene transfer mediated by the asialoglycoprotein receptor was covered by the high transfection efficiency of H1 itself. Similar results of a marginal increase in the transfection efficiency were obtained by conjugates of a short RGD sequence and H1. This unexpected failure in the receptor specificity of both conjugates could be due to the unspecific cell-binding capacity of the H1 moiety and to increasing solubility of the complexes as shown by gel shift and solubility measurements.
Haberland
A
A
Max Delbrück Center for Molecular Medicine, 13125 Berlin-Buch, Wiltbergstr. 50, Germany.
Dallüge
R
R
Zaitsev
S
S
Stahn
R
R
Böttger
M
M
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D004247:DNA / Q000378:metabolism*; D005690:Galactose / Q000378:metabolism; D006657:Histones / Q000737:chemistry / Q000378:metabolism*; D008024:Ligands; D009842:Oligopeptides; D012995:Solubility; D014162:Transfection
D006657:Histones; D008024:Ligands; D009842:Oligopeptides; C047981:arginyl-glycyl-aspartic acid; D004247:DNA; D005690:Galactose