Preliminary analysis of Sm14 in distinct fractions of Schistosoma mansoni adult worm extract.
In previous studies it was shown that the recombinant molecule, r-Sm14, induces high levels of protection against Schistosoma mansoni infection in two outbred animal models and immune crossprotection against infection by Fasciola hepatica in Swiss outbred mice. r-Sm14 was derived from a living worm extract, called SE, and is being developed as the molecular basis of an anti-helminth bivalent vaccine against the two parasites, for medical and veterinary application. Present data refer to SDS-PAGE and Western Blotting analysis of four different preparations of S. mansoni adult worms focusing Sm14 identification. The extracts correspond to the initial fraction of the SE extraction process, containing products released by living worms (SEi); SE2, reextraction of adult worms in PBS; and SE of separated male and female adult worms. In all extracts it was possible to detect the component of 14 kDa, that was recognized by specific anti-rSm14 antibody raised in rabbits.
Thaumaturgo
N
N
Laboratório de Esquistossomose Experimental, Departamento de Helmintologia, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, RJ, 21045-900, Brasil.
Vilar
M M
MM
Diogo
C M
CM
Edelenyi
R
R
Tendler
M
M
D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology; D015153:Blotting, Western; D002352:Carrier Proteins / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D050612:Fatty Acid Transport Proteins; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D005260:Female; D015801:Helminth Proteins / Q000032:analysis* / Q000276:immunology; D008297:Male; D026901:Membrane Transport Proteins; D051379:Mice; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D012550:Schistosoma mansoni / Q000737:chemistry* / Q000276:immunology; D012555:Schistosomiasis mansoni / Q000276:immunology*; D014612:Vaccines / Q000276:immunology
D000909:Antibodies, Helminth; D002352:Carrier Proteins; C082667:Fabp5 protein, mouse; C086936:Fabp7 protein, mouse; D050612:Fatty Acid Transport Proteins; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D015801:Helminth Proteins; D026901:Membrane Transport Proteins; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D014612:Vaccines; C068709:SM14 protein, Schistosoma mansoni