Isolation of a benzoate-utilizing Pseudomonas strain from soil and production of catechol from benzoate by transpositional mutants.
Pseudomonas sp. Ba-0511 was isolated from soil by enrichment cultivation on a medium containing 6 mg/ml of sodium benzoate. The bacterium could grow on a medium containing 20 mg/ml of sodium benzoate by a successive enrichment culture. One hundred and twelve transpositional mutants of the bacterium produced catechol from benzoate and accumulated it outside of the cells. Among the mutants, strain BA+63 produced a maximal amount of catechol (2.3 mg/ml) from 6 mg/ml of sodium benzoate after growing for 10.5 h. The conversion rate of benzoate to catechol was 50% on a molar basis. The catechol production by the resting cells increased in the presence of glycerol, and the maximal amount of catechol produced from 6 mg/ml of sodium benzoate reached 3.3 mg/ml at the conversion rate of 72% after 5 h of incubation. The resting cells converted m-methylbenzoic acid to 3- and 4-methylcatechol and m-chlorobenzoic acid to 3- and 4-chlorocatechol.
Wang
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CL
Division of Science of Biological Resources, The Graduate School of Science and Technology, Kobe University, Rokko, Japan.
Takenaka
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S
Murakami
S
S
Aoki
K
K
D001565:Benzoates / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002396:Catechols / Q000737:chemistry / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media; D004251:DNA Transposable Elements; D016254:Mutagenesis, Insertional; D011549:Pseudomonas / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism; D012988:Soil Microbiology; D013379:Substrate Specificity
D001565:Benzoates; D002396:Catechols; D003470:Culture Media; D004251:DNA Transposable Elements