Optical biosensors. Monitoring studies of glycopeptide antibiotic fermentation using white light interference.
This paper describes the design, characterization, and use of an optical biosensor suited for the process control of biotechnological processes. The detector principle is based on reflectometric interference spectroscopy (RIfS). RIfS enables a label-free, product-specific monitoring, with a future outline for on-line process control. The potential of the RIfS biosensor is exemplified by the qualitative and quantitative monitoring of the microbial production of vancomycin-type glycopeptide antibiotics.
Tünnemann
R
R
Institute of Organic Chemistry, Institute of Physical Chemistry, University of Tübingen, Germany.
Mehlmann
M
M
Süssmuth
R D
RD
Bühler
B
B
Pelzer
S
S
Wohlleben
W
W
Fiedler
H P
HP
Wiesmüller
K H
KH
Gauglitz
G
G
Jung
G
G
D000192:Actinomycetales / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000096:biosynthesis; D015374:Biosensing Techniques; D002851:Chromatography, High Pressure Liquid; D005285:Fermentation; D008027:Light; D009130:Muscle, Smooth; D010455:Peptides / Q000138:chemical synthesis
D000900:Anti-Bacterial Agents; D010455:Peptides