GD3 glycosphingolipid contributes to Fas-mediated apoptosis via association with ezrin cytoskeletal protein.
Efficiency of Fas-mediated apoptosis of lymphoid cells is regulated, among other means, by a mechanism involving its association with ezrin, a cytoskeletal protein belonging to the 4.1 family of proteins. In the present work, we provide evidence for a further molecule that associates to ezrin in Fas-triggered apoptosis, the disialoganglioside GD3. In fact, as an early event, GD3 redistributed in membrane-associated domains in uropods and co-localized with ezrin. Co-immunoprecipitation analyses confirmed this result, indicating a GD3-ezrin association. Altogether, these results are suggestive for a role of GD3 in Fas/ezrin-mediated apoptosis, supporting the view that uropods contain a multimolecular signaling complex involved in Fas-mediated apoptosis.
Giammarioli
A M
AM
Department of Ultrastructures, Istituto Superiore di Sanità, Rome, Italy.
Garofalo
T
T
Sorice
M
M
Misasi
R
R
Gambardella
L
L
Gradini
R
R
Fais
S
S
Pavan
A
A
Malorni
W
W
D017209:Apoptosis / Q000502:physiology*; D002460:Cell Line; D002855:Chromatography, Thin Layer; D003598:Cytoskeletal Proteins; D005732:Gangliosides / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D010750:Phosphoproteins / Q000378:metabolism*; D011233:Precipitin Tests; D019014:fas Receptor / Q000502:physiology*; D000099149:Ezrin
D003598:Cytoskeletal Proteins; D005732:Gangliosides; D010750:Phosphoproteins; D019014:fas Receptor; D000099149:Ezrin; C026226:ganglioside, GD3