Implantation of bacteria from human pulpal necrosis and translocation from root canals in gnotobiotic mice.
The aim of this study was to determine whether microorganisms recovered from infected human root canals were able to survive and translocate to a local lymph node when experimentally inoculated into the root canal system of germ-free mice. The microorganisms isolated from two patients with pulpal necrosis were inoculated in two groups of experimental animals; group I (Gemella morbillorum) and group II (Bifidobacterium adolescentis, Fusobacterium nucleatum, and Clostridium butyricum). G. morbillorum showed the highest frequency of colonization and translocation to the draining lymph node. In group II only F. nucleatum and C. butyricum colonized and translocated when inoculated in tri-association. When the bacteria from group II were inoculated in monoinfection all three species colonized the root canal of germ-free mice and translocated to the draining lymph node, but with different frequencies. We conclude that selective mechanisms occur in which some bacterial species are fit to survive, multiply, and translocate in the germ-free mouse model.
Ribeiro Sobrinho
A P
AP
Institute of Microbiology Professor Paulo de Góes, UFRJ, Rio de Janeiro, Brazil.
Lanna
M A
MA
Farias
L M
LM
Carvalho
M A
MA
Nicoli
J R
JR
de Uzeda
M
M
Vieira
L Q
LQ
D000818:Animals; D001421:Bacteria, Anaerobic / Q000254:growth & development* / Q000502:physiology*; D018988:Bacterial Translocation; D001430:Bacteriocins / Q000032:analysis; D001644:Bifidobacterium / Q000254:growth & development / Q000502:physiology; D003013:Clostridium / Q000254:growth & development / Q000502:physiology; D003786:Dental Pulp Cavity / Q000382:microbiology*; D003790:Dental Pulp Necrosis / Q000382:microbiology*; D004195:Disease Models, Animal; D016967:Fusobacterium nucleatum / Q000254:growth & development / Q000502:physiology; D005856:Germ-Free Life; D006095:Gram-Positive Cocci / Q000254:growth & development / Q000502:physiology; D006801:Humans; D008198:Lymph Nodes / Q000382:microbiology*; D051379:Mice
D001430:Bacteriocins