The effect of 2,5,2',5'-tetrachlorobiphenyl on growth and death of the cyanobacterium Anabaena flos-aquae.
The effect of 2,5,2',5'-tetrachlorobiphenyl (TcBP) on cell death was investigated in the cyanobacterium Anabaena flos-aquae (Lyngb.) Bréb grown in a P-limited, semicontinuous culture under N2-fixing conditions during a 17-d period. The TcBP was supplied daily to yield final concentrations of 1, 10, and 100 ppb (or ng/ml). At 1 and 10 ppb, the population size of live cells (sum of live vegetative cells and live heterocysts) was not different from that of the TcBP-free controls (p > 0.1, t test) and remained constant both in its absolute value (9.6 X 10(5) cells/ml) and as a percentage of the total population (98.6%). However, at 100 ppb, the population size of live cells declined significantly after the 11th day (8.2 x 10(5) cells/ml, or 96.6% of total population; p < 0.01, t test). The decrease in viable cells was mostly found in vegetative cells. Bioconcentration factor in the cell ([pg TcBP/g dry wt cells]/[pg TcBP/ml]) was 1.23 x 10(5).
Lee
D Y
DY
School of Public Health, State University of New York at Albany and Wadsworth Center, New York State Department of Health, 12201-0509, USA. keumsung@hanmail.net
Rhee
G Y
GY
D017033:Anabaena / Q000254:growth & development*; D018929:Cell Culture Techniques; D002470:Cell Survival; D004785:Environmental Pollutants / Q000009:adverse effects*; D011078:Polychlorinated Biphenyls / Q000009:adverse effects*; D011157:Population Dynamics; D014874:Water Pollutants, Chemical / Q000009:adverse effects*
D004785:Environmental Pollutants; D014874:Water Pollutants, Chemical; C009407:2,5,2',5'-tetrachlorobiphenyl; D011078:Polychlorinated Biphenyls