Amplified detection of DNA and analysis of single-base mismatches by the catalyzed deposition of gold on Au-nanoparticles.
A novel amplification route for DNA detection based on the deposition of gold on a 10 nm Au-colloid/avidin conjugate label acting as a 'seeding' catalyst, is described. Microgravimetric quartz-crystal-microbalance measurements are employed to transduce the catalyzed deposition of gold on the piezoelectric crystals. Three different DNA detection schemes are described: (i) analysis of a 27-base nucleic acid fragment; (ii) analysis of the entire M13phi DNA (7229 bases); and (iii) detection of a single-base mismatch in a DNA. Ultrasensitive detection of DNA is accomplished by the catalyzed deposition of gold, detection limit approximately 1 x 10(-15) M.
Weizmann
Y
Y
Institute of Chemistry, The Hebrew University of Jerusalem, Israel.
Patolsky
F
F
Willner
I
I
D020137:Base Pair Mismatch; D004247:DNA / Q000032:analysis* / Q000235:genetics; D006046:Gold; D006801:Humans
D006046:Gold; D004247:DNA