title: Evaluation of ultrastructural changes associated with encephalomyocarditis virus in the myocardium of experimentally infected piglets.
abstract: OBJECTIVE
  To evaluate the ultrastructural changes and localization of encephalomyocarditis virus (EMCV) and viral pathogenesis in the myocardium of experimentally infected piglets.
  
  
  METHODS
  Eight 20-day-old piglets.
  
  
  METHODS
  Six piglets were inoculated oronasally with 5 ml (10(6) median tissue culture infective dose/ml) of EMCV suspension, and 2 were used as uninfected controls. Piglets were euthanatized or died between postinoculation days 1 and 3. Samples of heart tissue from all piglets were evaluated histologically, by virus isolation, and by use of immunohistochemistry and electron microscopy.
  
  
  RESULTS
  All infected piglets had gross or microscopic lesions of interstitial myocarditis. immunohistochemically, EMCV antigen was detected in the cytoplasm of cardiac muscle cells, Purkinje fibers, and endothelial cells and in the nucleus of cardiac muscle cells and Purkinje fibers. Ultrastructural lesions were characterized by degeneration and necrosis of cardiac muscle cells and Purkinje fibers. Virus was present intracytoplasmically in cardiac muscle cells, Purkinje fibers, and endothelial cells of capillaries and intranuclearly in cardiac muscle cells. The cell membranes of the Purkinje fibers and endothelial cells had distinct protrusions that contained virus particles. In control piglets, no lesions were found, and no EMCV antigen was detected.
  
  
  CONCLUSIONS
  Localization of EMCV intracytoplasmically or intranuclearly in various myocardial cells may well reflect the sites of viral proliferation. The presence of virus particles in cell membrane protrusions and in vacuoles within the lumen of capillaries indicates that virus is released not only by disintegration of the host cell but also via exocytosis.
authors.affiliation: Laboratory of Pathology, Faculty of Veterinary Medicine, Aristotle University, Thessaloniki, Greece.
authors.forename: V
authors.initials: V
authors.lastname: Psychas
authors.forename: N
authors.initials: N
authors.lastname: Papaioannou
authors.forename: C
authors.initials: C
authors.lastname: Billinis
authors.forename: E
authors.initials: E
authors.lastname: Paschaleri-Papadopoulou
authors.forename: S
authors.initials: S
authors.lastname: Leontides
authors.forename: O
authors.initials: O
authors.lastname: Papadopoulos
authors.forename: T
authors.initials: T
authors.lastname: Tsangaris
authors.forename: J
authors.initials: J
authors.lastname: Vlemmas
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D009202:Cardiomyopathies / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D018188:Cardiovirus Infections / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D004686:Encephalomyocarditis virus; D006321:Heart / Q000821:virology*; D007150:Immunohistochemistry / Q000662:veterinary; D008854:Microscopy, Electron; D009206:Myocardium / Q000473:pathology / Q000648:ultrastructure*; D009500:Neutralization Tests / Q000662:veterinary; D013552:Swine; D013553:Swine Diseases / Q000473:pathology / Q000821:virology*
chemical_list: D000956:Antigens, Viral