New androgen response elements in the murine pem promoter mediate selective transactivation.
The Pem homeobox transcription factor is expressed under androgen control in the testis and epididymis. It is also transcribed in the ovary, muscle, and placenta. The mouse Pem gene promoter was cloned and sequenced. It was analyzed in transactivation tests using CV-1 and PC-3 cells expressing the AR and found to be strongly stimulated by androgens. EMSAs and mutational analysis of the Pem promoter allowed the identification of two functional androgen response elements named ARE-1 and ARE-2. They both differed from the consensus semipalindromic steroid response element and exhibited characteristics of direct repeats of the TGTTCT half-site. Unlike the steroid response element, both Pem androgen response elements were selectively responsive to androgen stimulation. Specific mutations in the left half-site of Pem ARE-1 and ARE-2, but not of the steroid response element, were still compatible with AR binding in the EMSA. In addition, Pem ARE-1, but not ARE-2 or the steroid response element, showed some flexibility with regard to spacing between half-sites. These results strongly suggest that the AR interacts differently with direct repeats than with inverted repeats, potentially leading to cis element-driven selective properties. Thus, the existence of several classes of DNA response elements might be an essential feature of differential androgen regulation.
Barbulescu
K
K
Research Laboratories of Schering AG, D-13342 Berlin, Germany.
Geserick
C
C
Schüttke
I
I
Schleuning
W D
WD
Haendler
B
B
D000728:Androgens / Q000494:pharmacology*; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D017124:Conserved Sequence; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D024202:Electrophoretic Mobility Shift Assay; D005938:Glucocorticoids / Q000494:pharmacology; D018398:Homeodomain Proteins / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D011372:Progestins / Q000494:pharmacology; D011401:Promoter Regions, Genetic; D051381:Rats; D011944:Receptors, Androgen / Q000378:metabolism; D020218:Response Elements; D016415:Sequence Alignment; D013329:Structure-Activity Relationship; D014157:Transcription Factors / Q000235:genetics*; D015533:Transcriptional Activation; D014162:Transfection
D000728:Androgens; D004247:DNA; D005938:Glucocorticoids; D018398:Homeodomain Proteins; D011372:Progestins; D011944:Receptors, Androgen; D014157:Transcription Factors; C490383:Rhox5 protein, rat; C498048:Rhox5 protein, mouse