A new functional domain of Bcl6 family that recruits histone deacetylases.
The proto-oncogene Bcl6 and its family gene, BAZF, encode a sequence-specific transcriptional repressor which contains the BTB/POZ domain in NH(2)-terminal region and zinc finger motifs in COOH-terminal region. The BTB/POZ domain and the middle portion of Bcl6 and BAZF are known to display transrepressor activity. Since we have identified the identical 17-amino acid (aa) sequence in the middle portion of Bcl6 and BAZF, the 17aa region may be another repressive domain of the middle portion. The reporter gene assay indicates that the 27aa sequence including the 17aa region recruits histone deacetylases to express transrepressor activity. Furthermore, overexpression of Bcl6 or Bcl6(POZ-) (Bcl6 deleted with the BTB/POZ domain) induced apoptosis in NIH3T3 cells, and the apoptosis was inhibited by the addition of histone deacetylase inhibitor in the culture. However, apoptosis was not induced in NIH3T3 cells by overexpression of Bcl6(POZ-) deleted with the 17aa region. These results indicate that the 17aa region in the middle portion of Bcl6 is a functional domain of transrepressor activity and is responsible for inducibility of apoptosis in NIH3T3 cells.
Zhang
H
H
Department of Developmental Genetics (H2), Graduate School of Medicine, Chiba University, Inohana 1-8-1, Chuo-ku, 260-8670, Chiba, Japan.
Okada
S
S
Hatano
M
M
Okabe
S
S
Tokuhisa
T
T
D016475:3T3 Cells; D000818:Animals; D017209:Apoptosis; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D006655:Histone Deacetylases / Q000378:metabolism*; D051379:Mice; D010957:Plasmids; D011518:Proto-Oncogene Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000737:chemistry / Q000235:genetics / Q000502:physiology; D051560:Proto-Oncogene Proteins c-bcl-6; D012097:Repressor Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D014162:Transfection; D051020:bcl-X Protein
C494812:Bcl2l1 protein, mouse; C113027:Bcl6b protein, mouse; D004268:DNA-Binding Proteins; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D051560:Proto-Oncogene Proteins c-bcl-6; D012097:Repressor Proteins; D014157:Transcription Factors; D051020:bcl-X Protein; D006655:Histone Deacetylases