title: Longitudinal variability of human immunodeficiency virus type 1 RNA viral load measurements by nucleic acid sequence-based amplification and NucliSens assays in a large multicenter study.
abstract: Human immunodeficiency virus type 1 (HIV-1) RNA measurements were evaluated within an externally controlled multilaboratory program. Three external standards (1.5 x 10(3) to 1.5 x 10(6) copies/ml) were included in 814 assay runs by four laboratories. Results indicate that HIV-1 RNA levels can be measured with a precision equal to that of the pre-highly active antiretroviral therapy era (standard deviations, +/-0.16 to 0.25 log10 units).
authors.affiliation: University of Southern California, Pediatrics, Los Angeles, California 90033, USA. marek@usc.edu
authors.forename: M J
authors.initials: MJ
authors.lastname: Nowicki
authors.forename: L
authors.initials: L
authors.lastname: Benning
authors.forename: J W
authors.initials: JW
authors.lastname: Bremer
authors.forename: W A
authors.initials: WA
authors.lastname: Meyer
authors.forename: C
authors.initials: C
authors.lastname: Hanson
authors.forename: D
authors.initials: D
authors.lastname: Brambilla
authors.forename: S
authors.initials: S
authors.lastname: Silver
authors.forename: A
authors.initials: A
authors.lastname: Kovacs
mesh_terms: D015658:HIV Infections / Q000175:diagnosis* / Q000821:virology; D015497:HIV-1 / Q000502:physiology*; D007753:Laboratories; D021141:Nucleic Acid Amplification Techniques / Q000379:methods / Q000592:standards*; D011786:Quality Control; D012367:RNA, Viral / Q000097:blood*; D012016:Reference Values; D021161:Self-Sustained Sequence Replication / Q000379:methods / Q000592:standards*; D019562:Viral Load; D014773:Virology
chemical_list: D012367:RNA, Viral