Crotalus durissus terrificus snake venom regulates macrophage metabolism and function.
In the present study, we examined the effect of Crotalus durissus terrificus venom on rat macrophage metabolism and function. Two hours after subcutaneous injection of the venom, peritoneal resident (unstimulated), elicited (thioglycollate-stimulated), and activated Mycobacterium bovis strain bacille Calmette Guérin (BCG) macrophages were collected, and their functional and metabolic parameters were analyzed. The venom inhibited spreading and phagocytosis of macrophages. On the other hand, this treatment increased H(2)O(2) and NO production, candidacidal activity, and the activities of key enzymes of glycolysis and glutaminolysis. We also investigated whether the venom could affect macrophage activation by thioglycollate or BCG. The administration of venom 2 h before injection of thioglycollate and BCG or 2 or 3 days after injection of the thioglycollate or BCG, respectively, did not modify the previous observations. These findings suggest that crotalic venom leads the macrophage to an activated state, with high production of oxygen- and nitrogen-reactive species. This cell activation state does not include inflammatory properties of spreading and phagocytosis.
Sampaio
S C
SC
Laboratory of Pathophysiology, Butantan Institute, São Paulo, Brazil.
Sousa-e-Silva
M C
MC
Borelli
P
P
Curi
R
R
Cury
Y
Y
D000818:Animals; D002176:Candida albicans / Q000502:physiology; D002452:Cell Count; D002478:Cells, Cultured; D003435:Crotalid Venoms / Q000494:pharmacology*; D017839:Crotalus; D005947:Glucose / Q000378:metabolism; D005973:Glutamine / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D008262:Macrophage Activation / Q000187:drug effects*; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism*; D008297:Male; D009163:Mycobacterium bovis / Q000502:physiology; D009569:Nitric Oxide / Q000096:biosynthesis; D010587:Phagocytosis / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D013864:Thioglycolates / Q000494:pharmacology; D013997:Time Factors
D003435:Crotalid Venoms; D013864:Thioglycolates; D005973:Glutamine; D009569:Nitric Oxide; D006861:Hydrogen Peroxide; D005947:Glucose