Isolation and expression of an ecdysteroid-inducible neutral endopeptidase 24.11-like gene in wing discs of Bombyx mori.
In the process of comparison of two cDNA libraries (W0, W2), we isolated a clone from the wing discs of Bombyx mori encoding a putative neutral endopeptidase 24.11-like gene. The predicted open reading frame encoded 772 amino acid residues, having about 53% identity with Drosophila GH07643, 36% with rat NEP, and 34% with rat ECE. This is the first NEP gene isolated in invertebrate. A 3.6-kb transcript was found to accumulate in the wing disc according to the increase of ecdysteroid titer during metamorphosis. Accumulation of the transcript was induced in wing discs with 20-hydroxyecdysone about 20h after incubation, which was inhibited by cycloheximide. This gene is ecdysone-inducible, appears to encode a functional protein, and may function during wing metamorphosis.
Zhao
X
X
Faculty of Agriculture, Utsunomiya University, 350 Mine, Utsunomiya, 321-8505, Tochigi, Japan.
Mita
K
K
Shimada
T
T
Okano
K
K
Quan
G X
GX
Kanke
E
E
Kawasaki
H
H
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D012831:Bombyx / Q000201:enzymology* / Q000235:genetics; D046508:Culture Techniques; D018076:DNA, Complementary; D004441:Ecdysterone / Q000494:pharmacology*; D015870:Gene Expression; D017344:Genes, Insect; D006801:Humans; D008969:Molecular Sequence Data; D015260:Neprilysin / Q000235:genetics* / Q000302:isolation & purification; D051381:Rats; D014921:Wings, Animal / Q000187:drug effects; D016335:Zinc Fingers
D018076:DNA, Complementary; D004441:Ecdysterone; C442434:endopeptidase 24.11, Bombyx; D015260:Neprilysin