Imaging postsynaptic activities of teleost thalamic neurons at single cell resolution using a voltage-sensitive dye.
Optical recording of neuronal activities using voltage-sensitive dyes (VSDs) is a useful method for simultaneous multi-site recording. However, it has been rather difficult to distinguish optical signals from individual, identified cells. We applied the optical recording technique using a high-speed charge coupled device (CCD) imaging system to a teleost thalamic nucleus, corpus glomerulosum (CG) which has a well-defined histological organization and large postsynaptic dendrites. Patch-like dye (di-4-ANEPPS) signals were observed in the dendritic layer of the CG in response to afferent nerve stimulations. These responses were completely blocked by an alpha-amino-3-hydroxy-5-methyl-4-isoxazole-proprionate (AMPA) receptor antagonist, did not propagate, and the size of the patches were close to that of a single dendritic tip of the 'large cell'. Thus, we found that these patch-like VSD signals most likely represent postsynaptic potentials at individual dendritic tips of the large cells.
Tsutsui
H
H
Misaki Marine Biological Station, Graduate School of Science, The University of Tokyo, Misaki, Miura, Kanagawa 238-0225, Japan.
Wolf
A M
AM
Knöpfel
T
T
Oka
Y
Y
D000818:Animals; D003712:Dendrites / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D001330:Electronic Data Processing / Q000295:instrumentation / Q000379:methods; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D005399:Fishes / Q000033:anatomy & histology / Q000502:physiology*; D005456:Fluorescent Dyes; D007091:Image Processing, Computer-Assisted / Q000295:instrumentation / Q000379:methods; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D011726:Pyridinium Compounds; D011810:Quinoxalines / Q000494:pharmacology; D018091:Receptors, AMPA / Q000037:antagonists & inhibitors / Q000378:metabolism; D013570:Synaptic Membranes / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D013788:Thalamus / Q000166:cytology / Q000187:drug effects / Q000502:physiology*
D018691:Excitatory Amino Acid Antagonists; D005456:Fluorescent Dyes; D011726:Pyridinium Compounds; D011810:Quinoxalines; D018091:Receptors, AMPA; C062865:2,3-dioxo-6-nitro-7-sulfamoylbenzo(f)quinoxaline; C050019:1-(3-sulfonatopropyl)-4-(beta)(2-(di-n-butylamino)-6-naphthylvinyl)pyridinium betaine