[Fertilization and subsequent development of cattle oocytes after reduced incubation with spermatozoa].
We studied the influence of the duration of joint incubation of cattle oocytes and spermatozoa (18 versus 1 h) on fertilization, cleavage, and embryonic development in vitro until the blastocyst stage. Spermatozoa of a bull of the Britanofrizskaya breed were used in the experiments. It was shown in the first experimental series that after a long-term incubation with the spermatozoa, the percentage of penetrated eggs increased: 71.7% and 56.0% (p < 0.05) after 18-hour and 1-hour incubation, respectively. However, no differences were found in the number of normally fertilized eggs: 46.5 and 39.0%, respectively. In the second experimental series, no significant differences were found in either the number of cleaving embryos (41.2 and 32.2%, respectively) or the capacity of cleaving embryos to develop in vitro until the blastocyst stage (21.7 and 15.8%, respectively). Thus, reduction in the time of joint incubation of cattle gametes upon in vitro fertilization to 1 h did not reduce the number of normally fertilized eggs and did not affect their capacity for subsequent in vitro development.
Krivokharchenko
A S
AS
All-Russian Institute of Physiology, Biochemistry and Nutrition of Farm Animals, Russia Academy of Agricultural Sciences, Borovsk, Kaluga Oblast, 249010 Russia.
Smetanina
I G
IG
Tatarinova
L V
LV
D000818:Animals; D001755:Blastocyst / Q000502:physiology*; D002417:Cattle; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D008297:Male; D009865:Oocytes / Q000502:physiology; D013084:Sperm-Ovum Interactions