Pentapeptide regulation of aspartyl-phosphate phosphatases.
Aspartyl-phosphate phosphatases are integral components of the phosphorelay signal transduction system for sporulation initiation in Bacillus subtilis. The Rap and Spo0E families of protein phosphatases specifically dephosphorylate the sporulation response regulators Spo0F and Spo0A, respectively. The phosphatases interpret regulatory signals antithetical to sporulation and the Rap phosphatases are subject to inactivation by specific pentapeptides generated from an inactive peptide precursor. Additional regulatory signals are brought about by the complex activation circuit that generates the Phr pentapeptide inhibitors of Rap phosphatases. Phr peptide's recognition of the Rap phosphatase targets is remarkably specific. Specificity is dictated by the amino acid sequence of the pentapeptide. The identification of tetratricopeptide repeats in the Rap proteins may explain the mechanism by which Phr peptides bind to and inhibit the activity of Rap phosphatases.
Perego
M
M
Division of Cellular Biology, Department of Molecular and Experimental Medicine, MEM-116, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA. mperego@scripps.edu
Brannigan
J A
JA
D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000502:physiology*; D001426:Bacterial Proteins / Q000037:antagonists & inhibitors* / Q000187:drug effects; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000378:metabolism*; D010675:Pheromones / Q000378:metabolism*; D010749:Phosphoprotein Phosphatases / Q000037:antagonists & inhibitors*; D012808:Sigma Factor; D015398:Signal Transduction / Q000502:physiology*; D013171:Spores, Bacterial / Q000378:metabolism; D014157:Transcription Factors
D001426:Bacterial Proteins; D009842:Oligopeptides; D010675:Pheromones; D012808:Sigma Factor; D014157:Transcription Factors; C110354:rap protein, bacteria; C092468:spoIIR protein, Bacillus subtilis; C030069:spore-specific proteins, Bacillus; D010749:Phosphoprotein Phosphatases