title: Effect of cyanide concentrations on the secondary structures of protein in the crude homogenates of the fish gill tissue.
abstract: The effect of cyanide concentrations on the secondary conformation of protein in the fish gill homogenate was determined using an attenuated total reflectance (ATR)/Fourier transform infrared (FT-IR) microspectroscopy. Gills from male Tilapia zillii were isolated and homogenized in pH 8.0 Tris buffer solution and subjected to FT-IR study. The results indicate that the amide I and III bands of protein in fish gill homogenate deformed markedly with the increase of cyanide concentration. The fish gill homogenate shows a maximum peak at 1650 cm(-1) in amide I band, suggesting the predominant proportion of alpha-helical conformation. Once the KCN was added into the gill homogenate, the maximum peak shifted gradually from 1650 to 1643 cm(-1) due to the random coil structure, with the increase of cyanide concentration used. Two additional shoulders at 1657 (alpha-helix) and 1627 (beta-sheet) cm(-1) also appeared gradually, implying that the cyanide can in part induce changes in protein conformation of fish gill homogenate from alpha-helix to random coil and beta-sheet conformations.
authors.affiliation: Biopharmaceutics Laboratory, Department of Medical Research and Education, Veterans General Hospital-Taipei, Taipei, Taiwan.
authors.forename: H L
authors.initials: HL
authors.lastname: Chu
authors.forename: T Y
authors.initials: TY
authors.lastname: Liu
authors.forename: S Y
authors.initials: SY
authors.lastname: Lin
mesh_terms: D000818:Animals; D029941:Fish Proteins / Q000737:chemistry / Q000187:drug effects*; D005880:Gills / Q000187:drug effects* / Q000473:pathology; D006856:Hydrogen Cyanide / Q000633:toxicity*; D008297:Male; D011489:Protein Denaturation / Q000187:drug effects; D017433:Protein Structure, Secondary / Q000187:drug effects; D017550:Spectroscopy, Fourier Transform Infrared; D017210:Tilapia; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D029941:Fish Proteins; D014874:Water Pollutants, Chemical; D006856:Hydrogen Cyanide