Oral cancer in vivo gene expression profiling assisted by laser capture microdissection and microarray analysis.
Large scale gene expression profiling was carried out on laser capture microdissected (LCM) tumor and normal oral epithelial cells and analysed on high-density oligonucleotide microarrays. About 600 genes were found to be oral cancer associated. These oral cancer associated genes include oncogenes, tumor suppressors, transcription factors, xenobiotic enzymes, metastatic proteins, differentiation markers, and genes that have not been implicated in oral cancer. The database created provides a verifiable global profile of gene expression during oral carcinogenesis, revealing the potential role of known genes as well as genes that have not been previously implicated in oral cancer.
Alevizos
I
I
Department of Oral Medicine and Diagnostic Sciences, Division of Oral Pathology, Harvard School of Dental Medicine, 188 Longwood Avenue, Boston, MA 02115, USA.
Mahadevappa
M
M
Zhang
X
X
Ohyama
H
H
Kohno
Y
Y
Posner
M
M
Gallagher
G T
GT
Varvares
M
M
Cohen
D
D
Kim
D
D
Kent
R
R
Donoff
R B
RB
Todd
R
R
Yung
C M
CM
Warrington
J A
JA
Wong
D T
DT
D000368:Aged; D000369:Aged, 80 and over; D056668:Cathepsin L; D002403:Cathepsins / Q000096:biosynthesis; D017364:Collagenases / Q000096:biosynthesis; D003546:Cysteine Endopeptidases; D018076:DNA, Complementary / Q000378:metabolism; D016208:Databases, Factual; D015536:Down-Regulation; D004847:Epithelial Cells / Q000473:pathology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D008954:Models, Biological; D009061:Mouth Mucosa / Q000473:pathology; D009062:Mouth Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D005810:Multigene Family; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012984:Software; D015854:Up-Regulation; D014568:Urokinase-Type Plasminogen Activator / Q000096:biosynthesis
D018076:DNA, Complementary; D002403:Cathepsins; D014568:Urokinase-Type Plasminogen Activator; D003546:Cysteine Endopeptidases; C534281:CTSL protein, human; D056668:Cathepsin L; D017364:Collagenases