[Determination of adenosine and 3'-deoxyadenosine in Cordyceps militaris (L.) Link. by HPLC].
The establishment of a quantitative determination method by HPLC for adenosine and 3'-deoxyadenosine in Cordyceps militaris is reported. The sample was extracted with water under ultrasonic management for 2 hours. Chromatographic conditions: YWG C18 10 microns 5 mm x 200 mm; mobile phase consisted of NaH2PO4-NaOH buffer solution (pH 6.8); UV detection at 260 nm. The recoveries of adenosine and 3'-deoxyadenosine were 95.8% +/- 0.64% and 100.6% +/- 0.65% (n = 5) respectively.
Guo
C
C
Changzheng Hospital, Second Military Medical University, Shanghai 200003.
Zhu
J
J
Zhang
C
C
Zhang
L
L
D000241:Adenosine / Q000032:analysis*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003839:Deoxyadenosines / Q000032:analysis*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D006999:Hypocreales / Q000737:chemistry* / Q000254:growth & development; D007915:Lepidoptera / Q000737:chemistry*
D003839:Deoxyadenosines; D004365:Drugs, Chinese Herbal; C058120:cordycepin; D000241:Adenosine