The adhesion molecules, l-selectin and sialyl lewis x, relate to the formation of the follicular dendritic cell-lymphocyte cluster in the mantle zone.
The follicular dendritic cell (FDC)-lymphocyte cluster is rich in the follicular light zone of the secondary lymphoid follicles (LFs). Although, the mantle zone (MZ) also has FDC-lymphocyte cluster, it has not known about what kind of adhesion molecules relates to cluster formation. In the present study, we investigated whether the adhesion molecules, L-selectin (CD62L) and sialyl Lewis x (CD15s) can mediate the formation of the cluster in human tonsillar LFs. The MZ only expressed both the adhesion molecules in the secondary LF. Isolated FDC-lymphocyte clusters were composed of CD62L(+) lymphocytes and CD15s(+) FDCs. Stamper-Woodruff binding assay revealed that the binding of IgD(+) lymphocytes was significantly inhibited by pretreatment with anti-CD62L antibody or with anti-CD15s antibody. These results indicate that CD62L on MZ lymphocytes and CD15s on FDCs may play a role of the cluster formation, unlike the clusters in the other parts of LFs.
Suzuki
A
A
The First Department of Pathology, Yamagata University School of Medicine, 2-2-2 Iida-Nishi, 990-9585, Yamagata, Japan.
Yamakawa
M
M
Tsukamoto
M
M
D000911:Antibodies, Monoclonal / Q000494:pharmacology; D001402:B-Lymphocytes / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D002448:Cell Adhesion / Q000187:drug effects; D002648:Child; D002675:Child, Preschool; D002918:Chymotrypsin / Q000378:metabolism; D020566:Dendritic Cells, Follicular / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D007072:Immunoglobulin D / Q000378:metabolism; D007150:Immunohistochemistry; D019041:L-Selectin / Q000378:metabolism*; D016256:Lewis X Antigen / Q000378:metabolism; D016253:Microscopy, Immunoelectron; D009844:Oligosaccharides / Q000378:metabolism*; D014066:Palatine Tonsil / Q000166:cytology* / Q000276:immunology* / Q000648:ultrastructure; D000080506:Sialyl Lewis X Antigen
D000911:Antibodies, Monoclonal; D007072:Immunoglobulin D; D016256:Lewis X Antigen; D009844:Oligosaccharides; D000080506:Sialyl Lewis X Antigen; D019041:L-Selectin; D002918:Chymotrypsin