title: Improved efficiency and reliability of RT-PCR using tag-extended RT primers and temperature gradient PCR.
authors.affiliation: Wageningen Centre for Food Sciences, The Netherlands. sybesma@nizo.nl
authors.forename: W
authors.initials: W
authors.lastname: Sybesma
authors.forename: J
authors.initials: J
authors.lastname: Hugenholtz
authors.forename: I
authors.initials: I
authors.lastname: Mierau
authors.forename: M
authors.initials: M
authors.lastname: Kleerebezem
mesh_terms: D017931:DNA Primers; D004094:Dihydropteroate Synthase / Q000235:genetics; D005189:False Positive Reactions; D005492:Folic Acid / Q000096:biosynthesis / Q000235:genetics; D006136:GTP Cyclohydrolase / Q000235:genetics; D013294:Lactococcus lactis / Q000235:genetics; D009876:Operon; D010453:Peptide Synthases / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D012329:RNA, Bacterial / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013696:Temperature
chemical_list: D017931:DNA Primers; D012329:RNA, Bacterial; D005492:Folic Acid; D004094:Dihydropteroate Synthase; D006136:GTP Cyclohydrolase; D010453:Peptide Synthases; C023103:folylpolyglutamate synthetase