title: Differential synaptic localization of GluR2 and EAAC1 in the macaque monkey entorhinal cortex: a postembedding immunogold study.
abstract: The synaptic distribution of alpha-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid receptor subunit GluR2 and neuronal glutamate transporter subunit EAAC1 were studied using immunogold in layer II of the macaque monkey entorhinal cortex. Immunoreactivity for EAAC1 and GluR2 was frequent at asymmetric synapses and their associated membrane. The synaptic localization of EAAC1 differed considerably from that of GluR2, in that GluR2 immunolabelling was most commonly located within the postsynaptic density, but EAAC1 localization was more heterogeneous and was predominant at the edge of postsynaptic densities and perisynaptic zones. Since EAAC1 may play an important role in clearing glutamate from the synaptic cleft and intercellular spaces, the high perisynaptic expression of EAAC1 in these neurons could presumably offer a powerful mechanism through which high concentrations of glutamate could be efficiently removed from the synapses following release and interaction with glutamate receptors. The distribution of EAAC1 may also offer protection for these neurons against excessive glutamatergic stimuli that may occur under certain pathological conditions.
authors.affiliation: Kastor Neurobiology of Aging Laboratories, Mount Sinai School of Medicine, One Gustave L. Levy Place, New York, NY 10029, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: He
authors.forename: P R
authors.initials: PR
authors.lastname: Hof
authors.forename: W G
authors.initials: WG
authors.lastname: Janssen
authors.forename: J D
authors.initials: JD
authors.lastname: Rothstein
authors.forename: J H
authors.initials: JH
authors.lastname: Morrison
mesh_terms: D027322:Amino Acid Transport System X-AG; D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D018728:Entorhinal Cortex / Q000378:metabolism* / Q000648:ultrastructure; D050491:Glutamate Plasma Membrane Transport Proteins; D018698:Glutamic Acid / Q000378:metabolism; D007150:Immunohistochemistry; D008252:Macaca fascicularis / Q000033:anatomy & histology / Q000378:metabolism*; D008297:Male; D008854:Microscopy, Electron; D009474:Neurons / Q000378:metabolism* / Q000648:ultrastructure; D018091:Receptors, AMPA / Q000378:metabolism*; D027981:Symporters; D013570:Synaptic Membranes / Q000378:metabolism* / Q000648:ultrastructure; D009435:Synaptic Transmission / Q000502:physiology
chemical_list: D027322:Amino Acid Transport System X-AG; D002352:Carrier Proteins; D050491:Glutamate Plasma Membrane Transport Proteins; D018091:Receptors, AMPA; D027981:Symporters; D018698:Glutamic Acid; C104722:glutamate receptor ionotropic, AMPA 2