Vdelta1 and Vdelta2 gammadelta T cells express distinct surface markers and might be developmentally distinct lineages.
We report here that the two major types of gammadelta T cells found in human blood, Vdelta1 and Vdelta2, were found to have markedly different phenotypes. Vdelta2 cells had a phenotype typical of most alphabeta T cells in blood; i.e., they were CD5(+), CD28(+), and CD57(-). In contrast, Vdelta1 cells tended to be CD5(-/dull), CD28(-), and CD57(+). Furthermore, although Vdelta1 T cells appeared to be "naive" in that they were CD45RA(+), they were CD62L(-) and on stimulation uniformly produced interferon-gamma, indicating that they are in fact memory/effector cells. This phenotype for Vdelta1 cells was similar to that of intestinal intraepithelial lymphocytes, a subset that can develop in the absence of the thymus. We suggest that the Vdelta1 and Vdelta2 T cell subsets represent distinct lineages with different developmental pathways. The disruption of the supply of normal, thymus-derived T cells in HIV-infected individuals might be responsible for the shift in the Vdelta2/Vdelta1 ratio that occurs in the blood of individuals with HIV disease.
De Rosa
S C
SC
Vaccine Research Center, National Institutes of Health, Bethesda, Maryland 20814, USA. SDeRosa@nih.gov
Mitra
D K
DK
Watanabe
N
N
Herzenberg
L A
LA
Herzenberg
L A
LA
Roederer
M
M
D000945:Antigens, Differentiation, T-Lymphocyte / Q000378:metabolism*; D018956:CD5 Antigens / Q000378:metabolism; D018959:CD57 Antigens / Q000378:metabolism; D019070:Cell Lineage; D016207:Cytokines / Q000096:biosynthesis; D015658:HIV Infections / Q000378:metabolism*; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000032:analysis*; D016130:Immunophenotyping; D017493:Leukocyte Common Antigens / Q000378:metabolism; D018655:Lymphocyte Count; D008562:Membrane Glycoproteins / Q000032:analysis; D054353:Perforin; D052899:Pore Forming Cytotoxic Proteins; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000032:analysis*; D016176:T-Lymphocyte Subsets / Q000145:classification* / Q000276:immunology; D013950:Thymus Gland / Q000254:growth & development
D000945:Antigens, Differentiation, T-Lymphocyte; D018956:CD5 Antigens; D018959:CD57 Antigens; D016207:Cytokines; D007135:Immunoglobulin Variable Region; D008562:Membrane Glycoproteins; D052899:Pore Forming Cytotoxic Proteins; D016692:Receptors, Antigen, T-Cell, gamma-delta; D054353:Perforin; D017493:Leukocyte Common Antigens