Stability of gastrointestinal glutathione peroxidase mRNA in selenium deficiency depends on its 3'UTR.
Selenoproteins decrease upon selenium-deprivation according to their hierarchical ranking. Whereas classical glutathione peroxidase (cGPx) responds to decreased selenium supply with a complete loss of protein and a marked reduction of mRNA levels, gastrointestinal glutathione peroxidase (GI-GPx) remains detectable and its mRNA is stable. The impact of the 3'UTR on cGPx and GI-GPx mRNA stability was studied in stably transfected HepG2 cells with combinations of mutually exchanged coding regions and 3'UTRs of human cGPx and GI-GPx. Stability of chimeric mRNAs was measured by competitive RT-PCR. We found that GI-GPx 3'UTR is sufficient to stabilize its own mRNA but not that of cGPx.
Wingler
K
K
German Institute of Human Nutrition and Institute of Nutritional Science, University of Potsdam, Potsdam-Rehbrücke, Germany.
Müller
C
C
Brigelius-Flohé
R
R
D020413:3' Untranslated Regions / Q000235:genetics*; D005979:Glutathione Peroxidase / Q000235:genetics*; D006801:Humans; D007700:Kinetics; D055503:Protein Multimerization; D011506:Proteins / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012643:Selenium / Q000172:deficiency*; D051140:Selenoproteins; D014162:Transfection; D014407:Tumor Cells, Cultured
D020413:3' Untranslated Regions; D011506:Proteins; D012333:RNA, Messenger; D011994:Recombinant Proteins; D051140:Selenoproteins; C117436:GPX2 protein, human; D005979:Glutathione Peroxidase; D012643:Selenium