High performance liquid chromatographic determination of dextromethorphan and its metabolite in human urine.
A reverse-phase high-performance liquid chromatographic method was established for the determination of dextromethorphan (DM) and its metabolite dextrophan (DX) in human urine. The sample was purified by an acid-base extraction followed by HPLC analysis on a phenyl column. The eluate was monitored by fluorescence at 280 nm (excitation) and 310 nm (emission). The limit of detection of DM was 0.023 mg.L-1. The within-day and between-day RSD% of DM and DX were all lower than 5%. The average recovery was 104.78%.
Cai
W M
WM
Department of Clinical Pharmacology, Nanjing General Hospital of Nanjing Command PLA, Nanjing 210002.
Chen
B
B
Chu
X
X
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003915:Dextromethorphan / Q000378:metabolism / Q000652:urine*; D003917:Dextrorphan / Q000652:urine*; D006801:Humans
D003917:Dextrorphan; D003915:Dextromethorphan