Oxidative stress activates ATMPK6, an Arabidopsis homologue of MAP kinase.
Mitogen-activated protein kinase (MAPK) cascades function in biotic and abiotic stress responses in plants. We analysed effect of oxidative stress on the activation of ATMPK6, an Arabidopsis thaliana MAPK, in Arabidopsis T87 cultured cells and rosette leaves using anti-ATMPK6 specific antibody. ATMPK6 in T87 cells was strongly activated by reactive oxygen species (ROS) such as H(2)O(2) and KO(2). In leaves, ATMPK6 was activated by paraquat and 3-amino-1,2,4-triazole (a catalase inhibitor). These results indicate that ATMPK6 is one of the candidates for signal mediators in response to abiotic or biotic sources for ROS in Arabidopsis.
Yuasa
T
T
Laboratory of Plant Molecular Biology, RIKEN Tsukuba Institute, 3-1-1 Koyadai, Tsukuba, Ibaraki, 305-0074 Japan.
Ichimura
K
K
Mizoguchi
T
T
Shinozaki
K
K
D000222:Adaptation, Physiological; D000640:Amitrole / Q000037:antagonists & inhibitors; D017360:Arabidopsis / Q000201:enzymology / Q000502:physiology*; D029681:Arabidopsis Proteins; D002374:Catalase / Q000187:drug effects; D004789:Enzyme Activation; D006540:Herbicides / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000378:metabolism; D020935:MAP Kinase Signaling System / Q000502:physiology; D020928:Mitogen-Activated Protein Kinases / Q000276:immunology / Q000378:metabolism*; D018384:Oxidative Stress / Q000502:physiology*; D010087:Oxides / Q000378:metabolism; D010269:Paraquat / Q000494:pharmacology; D017680:Potassium Compounds / Q000378:metabolism; D017382:Reactive Oxygen Species
D029681:Arabidopsis Proteins; D006540:Herbicides; D010087:Oxides; D017680:Potassium Compounds; D017382:Reactive Oxygen Species; C068440:potassium oxide; D006861:Hydrogen Peroxide; D002374:Catalase; C436293:MPK6 protein, Arabidopsis; D020928:Mitogen-Activated Protein Kinases; D010269:Paraquat; D000640:Amitrole