title: Immunisation with Salmonella typhimurium-delivered glyceraldehyde-3-phosphate dehydrogenase protects mice against challenge infection with Echinococcus multilocularis eggs.
abstract: Recombinant glyceraldehyde-3-phosphate dehydrogenase of the cestode parasite Echinococcus multilocularis was expressed in Escherichia coli and in Salmonella typhimurium. The potential of different forms of the recombinant antigen to protect BALB/c mice against oral challenge infections with E. multilocularis eggs was evaluated. Oral or intraperitoneal immunisation with live attenuated S. typhimurium as a carrier for recombinant glyceraldehyde-3-phosphate dehydrogenase of the E. multilocularis resulted in significant protection, reducing the number of developing metacestodes up to 79.8%. The sera of protected animals did not contain detectable amounts of antibody against glyceraldehyde-3-phosphate dehydrogenase of E. multilocularis. By contrast, although anti-glyceraldehyde-3-phosphate dehydrogenase of E. multilocularis antibodies were detectable in the sera, immunisation with E. coli-expressed recombinant glutathione-S-transferase-fusion protein or with glyceraldehyde-3-phosphate dehydrogenase of E. multilocularis fused to a 6HIS-tag failed to protect the animals against oral challenge infections. These data emphasise that antigen delivery systems play a critical role in vaccination and the induction of protective immunity against helminth parasites.
authors.affiliation: Division of Parasitology, Department of Zoology, University of Hohenheim, 70599 Stuttgart, Germany.
authors.forename: V
authors.initials: V
authors.lastname: Müller-Schollenberger
authors.forename: W
authors.initials: W
authors.lastname: Beyer
authors.forename: P
authors.initials: P
authors.lastname: Schnitzler
authors.forename: A
authors.initials: A
authors.lastname: Merckelbach
authors.forename: S
authors.initials: S
authors.lastname: Roth
authors.forename: B H
authors.initials: BH
authors.lastname: Kalinna
authors.forename: R
authors.initials: R
authors.lastname: Lucius
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D003001:Cloning, Molecular; D004443:Echinococcosis / Q000276:immunology* / Q000469:parasitology / Q000517:prevention & control; D004446:Echinococcus / Q000201:enzymology / Q000276:immunology*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005260:Female; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D012486:Salmonella typhimurium / Q000201:enzymology / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D018709:Statistics, Nonparametric
chemical_list: D011994:Recombinant Proteins; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases