Gold nanoparticle-based quantitative electrochemical detection of amplified human cytomegalovirus DNA using disposable microband electrodes.
An electrochemical DNA detection method has been developed for the sensitive quantification of an amplified 406-base pair human cytomegalovirus DNA sequence (HCMV DNA). The assay relies on (i) the hybridization of the single-stranded target HCMV DNA with an oligonucleotide-modified Au nanoparticle probe, (ii) followed by the release of the gold metal atoms anchored on the hybrids by oxidative metal dissolution, and (iii) the indirect determination of the solubilized AuIII ions by anodic stripping voltammetry at a sandwich-type screen-printed microband electrode (SPMBE). Due to the enhancement of the AuIII mass transfer by nonlinear diffusion during the electrodeposition time, the SPMBE allows the sensitive determination of AuIII in a small volume of quiescent solution. The combination of the sensitive AuIII determination at a SPMBE with the large number of AuIII released from each gold nanoparticle probe allows detection of as low as 5 pM amplified HCMV DNA fragment.
Authier
L
L
Laboratoire de Microbiologie Médicale et Moléculaire, Faculté de Médecine et d Pharmacie, Dijon, France.
Grossiord
C
C
Brossier
P
P
D003587:Cytomegalovirus / Q000235:genetics*; D015342:DNA Probes; D004277:DNA, Single-Stranded / Q000032:analysis*; D004279:DNA, Viral / Q000032:analysis*; D004563:Electrochemistry; D004566:Electrodes; D005784:Gene Amplification; D017979:Gold Colloid; D006801:Humans; D021141:Nucleic Acid Amplification Techniques; D009693:Nucleic Acid Hybridization / Q000379:methods; D015345:Oligonucleotide Probes
D015342:DNA Probes; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D017979:Gold Colloid; D015345:Oligonucleotide Probes