Plasma lithography--thin-film patterning of polymers by RF plasma polymerization II: Study of differential binding using adsorption probes.
In this study we present methods to physico-chemically modify micropatterned cell culture substrates that were manufactured using plasma lithography to incorporate affinity structures for specific cell binding. The surfaces consist of a pattern of a fluorocarbon plasma polymer with feature sizes between 5 and 100 microm on a background of a non-fouling tetraglyme (tetraethylene glycol dimethyl ether) plasma polymer. The tetraglyme polymer blocks virtually all non-specific binding of proteins, and it is non-adhesive for a fluorocarbon-polyethylene glycol (FC-PEG) surfactant designed to act as a 'hydrophobic anchor' for peptides. The surfactant shows a strong affinity for the fluorocarbon polymer pattern, thus enabling us to form a pattern of the surfactant-conjugated peptide. To verify this, we have synthesized a conjugate between histamine (as a model for a more complex peptide) and a commercially available FC-PEG surfactant. Disuccinimidyl carbonate was used to activate the terminal -OH group of the polyethylene glycol headgroup for the reaction with the amine-containing molecule. Affinity pattern formation can easily be achieved by immersion of the patterned substrates in a solution of the peptide-surfactant conjugate. Time of flight secondary ion mass spectroscopy in the imaging mode was used to verify that the surfactant localizes on the pattern, while the background remains bare. A model protein, bovine serum albumin, showed the same behavior. This suggests that these surfaces can be used for the formation of patterns of cell-adhesive proteins. These substrates will be used to investigate the influence of the cell size and shape of vascular smooth muscle cells on their physiology.
Goessl
A
A
Department of Bioengineering. University of Washington, Seattle 98195, USA.
Golledge
S L
SL
Hoffman
A S
AS
D000327:Adsorption; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000493:pharmacokinetics*; D001698:Biomedical Engineering; D002417:Cattle; D005026:Ethylene Glycols; D005465:Fluorocarbon Polymers / Q000737:chemistry / Q000493:pharmacokinetics*; D006632:Histamine / Q000737:chemistry / Q000378:metabolism / Q000493:pharmacokinetics; D018625:Microscopy, Atomic Force; D011092:Polyethylene Glycols / Q000737:chemistry / Q000378:metabolism / Q000493:pharmacokinetics; D011485:Protein Binding; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism / Q000493:pharmacokinetics; D018629:Spectrometry, Mass, Secondary Ion; D013057:Spectrum Analysis; D013499:Surface Properties
D001672:Biocompatible Materials; D005026:Ethylene Glycols; D005465:Fluorocarbon Polymers; D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols; C441631:tetraglyme; D006632:Histamine