title: Analysis of cytokines in the early development of gastric secondary lymphoid follicles in Helicobacter pylori-infected BALB/c mice with neonatal thymectomy.
abstract: Immunological interaction between the host and Helicobacter pylori seems to play a critical role in follicular formation in gastric mucosa. We reported H. pylori-induced follicular gastritis model using neonatally thymectomized mice. In this study, we investigated the involvement of various cytokines in this model. BALB/c mice were thymectomized on the third day after birth (nTx). At 6 weeks old, these mice were orally infected with H. pylori. Histological studies showed that follicular formation occurred from 8 weeks after the infection and that most of the infiltrating lymphocytes were CD4(+) and B cells. Neutrophils increased transiently at 1 week after the infection. Gamma interferon, interleukin-7 (IL-7), and IL-7 receptor were expressed in the stomach of the nTx mice irrespective of the infection. In contrast, expressions of the tumor necrosis factor alpha, IL-4 and lymphotoxin-alpha genes were remarkably upregulated by the infection. Our findings suggest that follicular formation may require cooperative involvement of a Th2-type immune response, tumor necrosis factor alpha and lymphotoxin-alpha in addition to the Th1-type immune response in H. pylori-induced gastritis in nTx mice.
authors.affiliation: Department of Gastroenterology and Endoscopic Medicine, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Uchida
authors.forename: K
authors.initials: K
authors.lastname: Okazaki
authors.forename: A
authors.initials: A
authors.lastname: Debrecceni
authors.forename: T
authors.initials: T
authors.lastname: Nishi
authors.forename: H
authors.initials: H
authors.lastname: Iwano
authors.forename: M
authors.initials: M
authors.lastname: Inai
authors.forename: S
authors.initials: S
authors.lastname: Uose
authors.forename: H
authors.initials: H
authors.lastname: Nakase
authors.forename: M
authors.initials: M
authors.lastname: Ohana
authors.forename: C
authors.initials: C
authors.lastname: Oshima
authors.forename: Y
authors.initials: Y
authors.lastname: Matsushima
authors.forename: C
authors.initials: C
authors.lastname: Kawanami
authors.forename: H
authors.initials: H
authors.lastname: Hiai
authors.forename: T
authors.initials: T
authors.lastname: Masuda
authors.forename: T
authors.initials: T
authors.lastname: Chiba
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D016207:Cytokines / Q000235:genetics*; D005260:Female; D005434:Flow Cytometry; D015870:Gene Expression; D016481:Helicobacter Infections / Q000276:immunology* / Q000473:pathology; D016480:Helicobacter pylori / Q000276:immunology*; D007124:Immunoenzyme Techniques; D008221:Lymphoid Tissue / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013270:Stomach / Q000276:immunology*; D013934:Thymectomy; D013997:Time Factors
chemical_list: D016207:Cytokines