title: Chemokine gene expression and clonal analysis of B cells in tissues involved by lymphoid interstitial pneumonitis from HIV-infected pediatric patients.
abstract: Lymphoid interstitial pneumonitis (LIP), a frequent pulmonary complication in HIV-infected pediatric patients, is characterized histologically by marked infiltration of lymphoid cells. We sought to evaluate the nature and pathogenesis of the lymphoid infiltrates and to examine the relationship of LIP to pulmonary MALT lymphoma that has been described in pediatric HIV positive patients. To examine the potential contribution of chemokines and cytokines to the inflammatory cell recruitment in tissues involved by lymphoid interstitial pneumonitis from HIV-infected pediatric patients, RNA was extracted from paraffin-embedded tissues from five lung biopsies in four pediatric HIV-positive patients and from five control, normal lung biopsies in five HIV-negative patients and was analyzed by semiquantitative RT-PCR for the expression of cytokines (TNF-alpha, GM-CSF, IFN-gamma, IL-4, IL-6, IL-10, and IL-18) and chemokines (IP-10, Mig, regulated upon activation, normal T expressed and secreted [RANTES], and MIP1-alpha and beta) after normalization for G3PDH. Expression of IL-18 was increased, as well as expression of IFN-gamma-inducible chemokines IP-10 and Mig in LIP tissues compared with controls. RANTES and MIP1-alpha and -beta were also increased in pediatric LIP lesions compared with controls. In contrast, expression of TNF-alpha, GM-CSF, IL-10, and IL-6 was variable in LIP tissues and controls. In addition, clonality of the B-cell population was evaluated by VDJ-PCR. A polyclonal B-cell population was shown in all five biopsies from five patients with LIP; and in one patient with concurrent LIP and MALT lymphoma, a band of increased intensity was observed in the LIP biopsy that was identical in size to the monoclonal band in the concurrent MALT lymphoma biopsy. These results provide evidence of high-level expression of certain chemokines in lymphoid interstitial pneumonitis tissues and suggest that chemokines and cytokines may play an important role in the recruitment of inflammatory cell infiltrates into these tissues. In addition, LIP may represent an early stage of MALT lymphoma or an immunologic response to a chronic antigenic stimulus that may provide a milieu or microenvironment for the evolution of a monoclonal B-cell population.
authors.affiliation: Laboratory of Pathology, Hematopathology Section, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA.
authors.forename: J
authors.initials: J
authors.lastname: Teruya-Feldstein
authors.forename: D W
authors.initials: DW
authors.lastname: Kingma
authors.forename: A
authors.initials: A
authors.lastname: Weiss
authors.forename: L
authors.initials: L
authors.lastname: Sorbara
authors.forename: P R
authors.initials: PR
authors.lastname: Burd
authors.forename: M
authors.initials: M
authors.lastname: Raffeld
authors.forename: B U
authors.initials: BU
authors.lastname: Mueller
authors.forename: G
authors.initials: G
authors.lastname: Tosato
authors.forename: E S
authors.initials: ES
authors.lastname: Jaffe
mesh_terms: D000293:Adolescent; D018951:Antigens, CD20 / Q000032:analysis; D001402:B-Lymphocytes / Q000378:metabolism / Q000473:pathology*; D017252:CD3 Complex / Q000032:analysis; D018925:Chemokines / Q000235:genetics*; D002648:Child; D002675:Child, Preschool; D002999:Clone Cells; D016207:Cytokines / Q000235:genetics; D005260:Female; D015870:Gene Expression; D015658:HIV Infections / Q000150:complications*; D006801:Humans; D007145:Immunoglobulin kappa-Chains / Q000032:analysis; D007146:Immunoglobulin lambda-Chains / Q000032:analysis; D007150:Immunohistochemistry; D017563:Lung Diseases, Interstitial / Q000150:complications / Q000235:genetics / Q000473:pathology*; D008175:Lung Neoplasms / Q000150:complications / Q000235:genetics / Q000473:pathology; D018442:Lymphoma, B-Cell, Marginal Zone / Q000150:complications / Q000235:genetics / Q000473:pathology; D008297:Male; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D018951:Antigens, CD20; D017252:CD3 Complex; D018925:Chemokines; D016207:Cytokines; D007145:Immunoglobulin kappa-Chains; D007146:Immunoglobulin lambda-Chains; D012333:RNA, Messenger