Relative importance of NF-kappaB p50 in mycobacterial infection.
To understand the role of NF-kappaB in the development of murine tuberculosis in vivo, NF-kappaB p50 knockout mice were infected with Mycobacterium tuberculosis by placing them in the exposure chamber of an airborne-infection apparatus. These mice developed multifocal necrotic pulmonary lesions or lobar pneumonia. Compared with the levels in wild-type mice, pulmonary inducible nitric oxide synthase, interleukin-2 (IL-2), gamma interferon, and tumor necrosis factor alpha mRNA levels were significantly low but expression of IL-10 and transforming growth factor beta mRNAs were within the normal ranges. The pulmonary IL-6 mRNA expression level was higher. Therefore, NF-kappaB and its interaction with host cells play an important role in the pathogenesis of tuberculosis.
Yamada
H
H
Department of Molecular Pathology, The Research Institute of Tuberculosis, Tokyo 204-0022, Japan.
Mizuno
S
S
Reza-Gholizadeh
M
M
Sugawara
I
I
D000818:Animals; D016207:Cytokines / Q000235:genetics; D004195:Disease Models, Animal; D015870:Gene Expression; D008168:Lung / Q000382:microbiology / Q000473:pathology / Q000648:ultrastructure; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008854:Microscopy, Electron / Q000379:methods; D009169:Mycobacterium tuberculosis / Q000254:growth & development / Q000276:immunology; D016328:NF-kappa B / Q000235:genetics / Q000276:immunology*; D052002:NF-kappa B p50 Subunit; D019001:Nitric Oxide Synthase / Q000235:genetics; D052247:Nitric Oxide Synthase Type II; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013154:Spleen / Q000382:microbiology / Q000473:pathology; D014397:Tuberculosis, Pulmonary / Q000276:immunology* / Q000473:pathology
D016207:Cytokines; D016328:NF-kappa B; D052002:NF-kappa B p50 Subunit; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse