title: Follicular fluid enhances sperm attraction and its motility in human.
abstract: OBJECTIVE
  Follicular fluid has a pivotal effect on motility and chemotaxis of spermatozoa for successful fertilization. The effect of human follicular fluid (hFF) and progesterone on attraction and motility of spermatozoa were investigated using simplified capillary assays.
  
  
  METHODS
  Capillary tubes loaded with hFF, modified human tubal fluid (m-hTF), or m-hTF supplemented with progesterone, respectively, were used for assessments of attraction and motility of spermatozoa following culture at various time intervals.
  
  
  RESULTS
  Number and motile ratio of spermatozoa in the tubes loaded with hFF were significantly (P < .05) higher than those with m-hTF. In the tubes loaded with m-hTF, m-hTF supplemented with progesterone, and hFF, the attracted number of spermatozoa were 34 x 10(5), 131 x 10(5), and 108 x 10(5), and motile ratio of spermatozoa was 37, 48, and 82%, respectively.
  
  
  CONCLUSIONS
  We conclude that hFF clearly plays a crucial role in enhancing attraction and motility of spermatozoa, and progesterone has strong effect on attraction of spermatozoa.
authors.affiliation: Kaya Mother-Child's Hospital, Chinju, South Korea.
authors.forename: B G
authors.initials: BG
authors.lastname: Jeon
authors.forename: J S
authors.initials: JS
authors.lastname: Moon
authors.forename: K C
authors.initials: KC
authors.lastname: Kim
authors.forename: H J
authors.initials: HJ
authors.lastname: Lee
authors.forename: S Y
authors.initials: SY
authors.lastname: Choe
authors.forename: G J
authors.initials: GJ
authors.lastname: Rho
mesh_terms: D000328:Adult; D005260:Female; D015571:Follicular Fluid / Q000502:physiology*; D006801:Humans; D008297:Male; D011374:Progesterone / Q000494:pharmacology*; D013081:Sperm Motility / Q000187:drug effects*; D013094:Spermatozoa / Q000187:drug effects / Q000502:physiology
chemical_list: D011374:Progesterone