title: [Contrivance for frozen section diagnosis of early minute lung cancer during operation].
abstract: We reported the technique of pathological diagnosis for minute lung cancer lesion of early stage during operation. Target lesion for our technique may be less than 1 cm in size and not be detected by palpation. Our technique may superior to usual method of frozen section of tissue specimen without fixation. At first, resected lung specimen was fixed by injection of 20% formalin solution subsequent warming by microwave oven for 40 seconds. Almost all lesions may be detected in proper thickness of specimen. Specimen was washed out and steeped in the 20% of sucrose solution for 30 to 60 seconds in order to avoid crystallization of water that was contained within lung specimen. Then thin sliced section of specimen was made by Cryostat in usual method. The minute cancer lesion was able to detect more easy than usual method of making frozen section of specimen. Formalin fixed and sucrose steeped frozen section may be able to evaluate the surgical margin from cancer exactly than the section made by non-fixed specimen.
authors.affiliation: Department of Thoracic Surgery, Hokushin General Hospital, Nakano, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Hosoda
authors.forename: N
authors.initials: N
authors.lastname: Shinohara
authors.forename: N
authors.initials: N
authors.lastname: Kojima
authors.forename: K
authors.initials: K
authors.lastname: Maruyama
authors.forename: Y
authors.initials: Y
authors.lastname: Handa
authors.forename: M
authors.initials: M
authors.lastname: Uchida
authors.forename: H
authors.initials: H
authors.lastname: Koike
authors.forename: Y
authors.initials: Y
authors.lastname: Kobayashi
mesh_terms: D005629:Frozen Sections / Q000379:methods*; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology* / Q000601:surgery