Cell culture test system for express analysis of cytotoxic and growth-stimulating effects of bioactive compounds.
Cultures of human and mammalian cells presenting 4 types of differentiation (normal human fibroblasts and myoblasts, human and Syrian hamster hepatoma cells, and mouse/mouse hybridoma cells) were used in a panel biotest system. This system allowed to evaluate the cytotoxic and stimulatory effect of bioactive compounds by determining the dose-effect relationships and some quantitative parameters including LD(50). Examination of some biolactive compounds of different nature (sangviritrin, escin, deltostim, cycloheximide, dexamethasone) confirmed high efficacy of this biotest system.
Chernikov
V G
VG
Medical Genetic Research Center, Russian Academy of Medical Sciences; Research Center of Biomedical Technologies, Moscow.
Terekhov
S M
SM
Krokhina
T B
TB
Shishkin
S S
SS
Smirnova
T D
TD
Adnoral
N V
NV
Rebrov
L B
LB
Denisov-Nikol'skii
Y I
YI
Bykov
B A
BA
D000818:Animals; D001681:Biological Assay; D018929:Cell Culture Techniques; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D006224:Cricetinae; D016903:Drug Monitoring / Q000379:methods*; D006801:Humans; D051379:Mice