Beta-scorpion toxin induces the release of gamma-[3 H]aminobutyric acid in rat brain slices.
The effect of the beta-scorpion toxin, TiTX gamma on the release of [3H]GABA from rat brain cortical slices is described. The stimulatory effect of TiTX gamma on the release of [3H]GABA was dependent on incubation time and TiTX gamma concentration with an EC50 of 0.19 microM. The scorpion toxin effect was calcium dependent and was completely inhibited by tetrodotoxin. beta-Alanine also induced the release of [3H]GABA and this effect was not inhibited by tetrodotoxin but was additive in the presence of TiTX gamma. The data suggest a neuronal origin for the release of [3H]GABA by TiTX gamma.
Fernandes
V M
VM
Laboratório de Neurofarmacologia, Departamento de Farmacologia, Faculdade de Medicina, ICB-UFMG, Av. Antonio Carlos 6627-Pampulha, 31270-901-Belo Horizonte MG, Brazil.
Nicolato
R
R
Moraes-Santos
T
T
Gomez
R S
RS
Prado
M A
MA
Romano-Silva
M A
MA
Gomez
M V
MV
D000818:Animals; D002118:Calcium / Q000378:metabolism; D020013:Calcium Signaling / Q000187:drug effects / Q000502:physiology; D002540:Cerebral Cortex / Q000187:drug effects* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions / Q000502:physiology; D004533:Egtazic Acid / Q000494:pharmacology; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D009498:Neurotoxins / Q000494:pharmacology*; D009924:Organ Culture Techniques; D011189:Potassium Chloride / Q000494:pharmacology; D051381:Rats; D012604:Scorpion Venoms; D015222:Sodium Channels / Q000187:drug effects* / Q000378:metabolism; D013570:Synaptic Membranes / Q000187:drug effects* / Q000378:metabolism; D013779:Tetrodotoxin / Q000494:pharmacology; D014316:Tritium / Q000493:pharmacokinetics; D015091:beta-Alanine / Q000494:pharmacology; D005680:gamma-Aminobutyric Acid / Q000378:metabolism* / Q000493:pharmacokinetics
D009498:Neurotoxins; D012604:Scorpion Venoms; D015222:Sodium Channels; C119038:Ts-gamma neurotoxin; D014316:Tritium; D015091:beta-Alanine; D013779:Tetrodotoxin; D004533:Egtazic Acid; D005680:gamma-Aminobutyric Acid; D011189:Potassium Chloride; D002118:Calcium