Molecular events involved in neuronal death induced in the mouse hippocampus by in-vivo injection of kainic acid.
Apoptosis results from the activation of a programmed cellular cascade involving several mechanisms. In the present study, we have investigated the implication of three molecules of this cascade, p53, Bax and caspase-3, in neuronal death induced by kainic acid (KA) administration in mouse hippocampus. Using immunocytochemistry, western blot and quantification of enzyme activity, we observed in p53+/+ and p53-/- animals that KA induced neuronal death by both p53-dependent and independent pathways. Moreover, apoptosis (labeled by TUNEL) and the increase of bax and caspase-3 protein expression after the neurotoxic insult appeared to clearly depend on p53 expression.
Djebaïli
M
M
CNRS UPR 9023, CCIPE, 34094 Montpellier 5, Cedex, France. djebaili@ccipe.montp.inserm.fr
Lerner-Natoli
M
M
Montpied
P
P
Baille
V
V
Bockaert
J
J
Rondouin
G
G
Pascale
M
M
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3; D020169:Caspases / Q000235:genetics / Q000502:physiology*; D053938:DNA Fragmentation; D018690:Excitatory Amino Acid Agonists / Q000633:toxicity*; D005786:Gene Expression Regulation / Q000187:drug effects; D016158:Genes, p53; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D020287:In Situ Nick-End Labeling; D007608:Kainic Acid / Q000633:toxicity*; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D008822:Mice, Transgenic; D009336:Necrosis; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000502:physiology*; D009474:Neurons / Q000737:chemistry / Q000187:drug effects* / Q000473:pathology; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000502:physiology*; D019253:Proto-Oncogene Proteins c-bcl-2; D016159:Tumor Suppressor Protein p53 / Q000502:physiology*; D051028:bcl-2-Associated X Protein
C494827:Bax protein, mouse; D018690:Excitatory Amino Acid Agonists; D009419:Nerve Tissue Proteins; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D016159:Tumor Suppressor Protein p53; D051028:bcl-2-Associated X Protein; C505409:Casp3 protein, mouse; D053148:Caspase 3; D020169:Caspases; D007608:Kainic Acid