title: Alpha1-adrenergic receptor subtypes in peripheral blood lymphocytes of essential hypertensives.
abstract: OBJECTIVE
  The expression of alpha1-adrenergic receptor subtypes in peripheral blood lymphocytes was investigated in 28 essential hypertensive patients as well as in the peripheral blood lymphocytes and aorta of spontaneously hypertensive rats (SHR) and normotensive Wistar-Kyoto (WKY) rats.
  
  
  METHODS
  Alpha1-adrenergic receptors were quantified by radioligand binding assays, employing [3H]-prazosin as the radioligand in association with compounds displaying different degrees of selectivity for alpha1A-, alpha1B- and alpha1D-adrenergic receptor subtypes.
  
  
  RESULTS
  The affinity of [3H]-prazosin binding was similar in peripheral blood lymphocytes of different stage essential hypertensive and normotensive subjects or of SHR and age-matched normotensive WKY rats as well as in the aortas of SHR and WKY rats. The radioligand binding assay revealed no change in the expression of alpha1-adrenergic receptors in peripheral blood lymphocytes of essential hypertensives compared with normotensive subjects; a moderate decrease of alpha1B-adrenergic receptors and an increase of alpha1D-adrenergic receptors. The relative densities of the alpha1-adrenergic receptor subtypes were similar in the three groups of essential hypertensives. In peripheral blood lymphocytes and in aorta of SHR, [3H]-prazosin binding was significantly reduced compared with normotensive WKY rats. The expression of alpha1-adrenergic receptor subtypes in peripheral blood lymphocytes of SHR was similar to that found in peripheral blood lymphocytes of essential hypertensives.
  
  
  CONCLUSIONS
  Changes of lymphocyte alpha1-adrenergic receptor subtypes in essential hypertensives are similar to those observed in lymphocytes and vascular tissues of animal models of hypertension. This suggests that assays of lymphocyte alpha1-adrenergic receptors may represent an indirect marker of their involvement in essential hypertension.
authors.affiliation: Department of Medicine and Experimental Oncology, Hypertension Unit, University of Turin, Turin, Italy. centroiperten.torino@libero.it
authors.forename: F
authors.initials: F
authors.lastname: Veglio
authors.forename: S K
authors.initials: SK
authors.lastname: Tayebati
authors.forename: D
authors.initials: D
authors.lastname: Schiavone
authors.forename: A
authors.initials: A
authors.lastname: Ricci
authors.forename: P
authors.initials: P
authors.lastname: Mulatero
authors.forename: E
authors.initials: E
authors.lastname: Bronzetti
authors.forename: F
authors.initials: F
authors.lastname: Rabbia
authors.forename: F
authors.initials: F
authors.lastname: Amenta
mesh_terms: D000328:Adult; D000818:Animals; D001011:Aorta; D005260:Female; D006801:Humans; D006973:Hypertension / Q000097:blood*; D008214:Lymphocytes / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D011224:Prazosin / Q000378:metabolism; D020033:Protein Isoforms / Q000097:blood; D011869:Radioligand Assay; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D011942:Receptors, Adrenergic, alpha / Q000097:blood*; D012016:Reference Values
chemical_list: D020033:Protein Isoforms; D011942:Receptors, Adrenergic, alpha; D011224:Prazosin