Secretion of the rotavirus VP8* protein in Lactococcus lactis.
Secretion of the VP8* subunit of the VP4 capsid protein of rotavirus by Lactococcus lactis has been achieved. For this purpose, a secretion vector has been constructed with the lactococcal signal sequence AL9 and the VP8*-encoding gene fragment. The amount of VP8* secreted by L. lactis in the culture supernatant was quantified and visualised by Western blot. Furthermore, it was shown to retain its hemagglutination capability, indicating that the conformation of the secreted peptide may be retaining its biological activity.
Gil
M T
MT
Departamento de Microbiologia y Ecologia, Hospital Clinico Universitario y Facultad de Medicina, Universidad de Valencia, Valencia, Spain.
Pérez-Arellano
I
I
Buesa
J
J
Pérez-Martínez
G
G
D000595:Amino Acid Sequence; D001483:Base Sequence; D015153:Blotting, Western; D006384:Hemagglutination; D013294:Lactococcus lactis / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D021382:Protein Sorting Signals / Q000235:genetics; D016601:RNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism*; D012401:Rotavirus / Q000235:genetics*; D017361:Viral Nonstructural Proteins / Q000235:genetics* / Q000378:metabolism*
D021382:Protein Sorting Signals; D016601:RNA-Binding Proteins; D017361:Viral Nonstructural Proteins; C079234:NS35 protein, rotavirus