title: Reduction of linoleic acid inhibition in production of conjugated linoleic acid by Propionibacterium freudenreichii ssp. shermanii.
abstract: A method for the production of conjugated linoleic acid (CLA) from linoleic acid (LA) using growing cultures of Propionibacterium freudenreichii ssp. shermanii JS was developed. The growth inhibitory effect of LA was eliminated by dispersing it in a sufficient concentration of polyoxyethylene sorbitan monooleate detergent. For the whey permeate medium used, the optimum LA:detergent ratio was 1:15 (w/w). As a result, the cultures tolerated at least 1000 microg x mL(-1) LA, which was converted to CLA with 57%-87% efficiency. The cis-9, trans-11 and trans-9, cis-11 isomers constituted 85%-90% of the CLA produced. The feasibility of the method was demonstrated also in de Man Rogosa-Sharpe (MRS) broth.
authors.affiliation: Helsinki University of Technology, Department of Chemical Technology, Finland. auli.rainio@hut.fi
authors.forename: A
authors.initials: A
authors.lastname: Rainio
authors.forename: M
authors.initials: M
authors.lastname: Vahvaselkä
authors.forename: T
authors.initials: T
authors.lastname: Suomalainen
authors.forename: S
authors.initials: S
authors.lastname: Laakso
mesh_terms: D001431:Bacteriological Techniques; D003470:Culture Media; D003902:Detergents; D019787:Linoleic Acid / Q000737:chemistry* / Q000378:metabolism* / Q000494:pharmacology; D008894:Milk Proteins; D011424:Propionibacterium / Q000187:drug effects / Q000254:growth & development*; D000067816:Whey Proteins
chemical_list: D003470:Culture Media; D003902:Detergents; D008894:Milk Proteins; D000067816:Whey Proteins; D019787:Linoleic Acid