[The effect of intestinal ischemia/reperfusion on increased sensitivity to endotoxin and its potential mechanism].
OBJECTIVE
To investigate the effect of intestinal ischemia/reperfusion injury (I/R) on increased sensitivity to endotoxin and its potential mechanism(s).


METHODS
Sprague-Dawley rats underwent 45 minutes of superior mesenteric artery occlusion followed by reperfusion. Twelve hours after reperfusion, endotoxin (Escherichia coli LPS, 1.5 mg/kg) was injected intravenously and rats were killed 12 hours later for measurement of organ function parameters. Also, in vitro study was performed to determine LPS-induced tumor necrosis factor (TNF)release in whole blood.


RESULTS
LPS injection after I/R resulted in marked hemodynamic dysfunction and multiple organ damage(P < 0.01), while no significant or only minor changes in organ function parameters were observed in both I/R and LPS groups. In whole blood, monoclonal antibody to CD14 significantly blocked the release of TNF at low LPS concentration (< or = 10 ng/ml), and this response was dose dependent. Moreover, TNF release in LPS-stimulated whole blood obtained after I/R was inhibited to a significantly greater degree than that in baseline blood samples or sham-operation group(P < 0.05-0.01).


CONCLUSIONS
Splanchnic artery occlusion followed by reperfusion could lead to increased sensitivity to the subsequent LPS challenge, which may be associated with a shift toward CD14-dependent mechanism(s).
Yao
Y
Y
Burn Institute, 304th Hospital of People's Liberation Army, Beijing 100037.
Yu
Y
Y
Chen
J
J
D000818:Animals; D000911:Antibodies, Monoclonal / Q000097:blood; D004926:Escherichia coli; D007421:Intestine, Small / Q000098:blood supply*; D018950:Lipopolysaccharide Receptors / Q000276:immunology*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008297:Male; D009102:Multiple Organ Failure / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism* / Q000503:physiopathology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D000911:Antibodies, Monoclonal; D018950:Lipopolysaccharide Receptors; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha