title: Role of free radicals in the toxicity of airborne fine particulate matter.
abstract: Exposure to airborne fine particles (PM2.5) is implicated in excess of 50 000 yearly deaths in the USA as well as a number of chronic respiratory illnesses. Despite intense interest in the toxicity of PM2.5, the mechanisms by which it causes illnesses are poorly understood. Since the principal source of airborne fine particles is combustion and combustion sources generate free radicals, we suspected that PM2.5 may contain radicals. Using electron paramagnetic resonance (EPR), we examined samples of PM2.5 and found large quantities of radicals with characteristics similar to semiquinone radicals. Semiquinone radicals are known to undergo redox cycling and ultimately produce biologically damaging hydroxyl radicals. Aqueous extracts of PM2.5 samples induced damage to DNA in human cells and supercoiled phage DNA. PM2.5-mediated DNA damage was abolished by superoxide dismutase, catalase, and deferoxamine, implicating superoxide radical, hydrogen peroxide, and the hydroxyl radical in the reactions inducing DNA damage.
authors.affiliation: Department of Chemistry, Louisiana State University, Baton Rouge, Louisiana 70803, USA.
authors.forename: B
authors.initials: B
authors.lastname: Dellinger
authors.forename: W A
authors.initials: WA
authors.lastname: Pryor
authors.forename: R
authors.initials: R
authors.lastname: Cueto
authors.forename: G L
authors.initials: GL
authors.lastname: Squadrito
authors.forename: V
authors.initials: V
authors.lastname: Hegde
authors.forename: W A
authors.initials: WA
authors.lastname: Deutsch
mesh_terms: D000393:Air Pollutants / Q000633:toxicity*; D001435:Bacteriophages; D002374:Catalase / Q000378:metabolism; D020552:Comet Assay; D004249:DNA Damage; D004278:DNA, Superhelical / Q000187:drug effects*; D003676:Deferoxamine / Q000737:chemistry; D004578:Electron Spin Resonance Spectroscopy; D004784:Environmental Monitoring; D005609:Free Radicals / Q000737:chemistry / Q000633:toxicity*; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D007951:Leukemia, Myeloid; D016877:Oxidants / Q000737:chemistry; D010316:Particle Size; D013482:Superoxide Dismutase / Q000378:metabolism; D014407:Tumor Cells, Cultured
chemical_list: D000393:Air Pollutants; D004278:DNA, Superhelical; D005609:Free Radicals; D016877:Oxidants; D006861:Hydrogen Peroxide; D002374:Catalase; D013482:Superoxide Dismutase; D003676:Deferoxamine