Assessing the diversity of marine bacterial beta-glucosidases by capillary electrophoresis zymography.
We propose a new method for the fast separation and detection of beta-glucosidases in environmental samples. With this approach, beta-glucosidases extracted from bacteria are evidenced by substrate-incorporated capillary electrophoresis (CE zymography) and their kinetic parameters can be determined by repeated injections using different substrate concentrations. Preliminary results obtained with natural bacterial communities from the coastal North Sea suggest that the diversity of beta-glucosidases in the marine environment might be much higher than previously observed.
Arrieta
J M
JM
Department of Biological Oceanography, Netherlands Institute for Sea Research, 1790 AB Den Burg, Texel, The Netherlands.
Herndl
G J
GJ
D019075:Electrophoresis, Capillary / Q000379:methods; D007700:Kinetics; D020560:Proteobacteria / Q000201:enzymology*; D012623:Seawater / Q000382:microbiology*; D001617:beta-Glucosidase / Q000145:classification* / Q000302:isolation & purification / Q000378:metabolism*
D001617:beta-Glucosidase