title: Molecular characterization of DDX26, a human DEAD-box RNA helicase, located on chromosome 7p12.
abstract: DEAD-box proteins comprise a family of ATP-dependent RNA helicases involved in several aspects of RNA metabolism. Here we report the characterization of the human DEAD-box RNA helicase DDX26. The gene is composed of 14 exons distributed over an extension of 8,123 bp of genomic sequence and encodes a transcript of 1.8 kb that is expressed in all tissues evaluated. The predicted amino acid sequence shows a high similarity to a yeast DEAD-box RNA helicase (Dbp9b) involved in ribosome biogenesis. The new helicase maps to 7p12, a region of frequent chromosome amplifications in glioblastomas involving the epidermal growth factor receptor (EGFR) gene. Nevertheless, co-amplification of DDX26 with EGFR was not detected in nine tumors analyzed.
authors.affiliation: Instituto Ludwig de Pesquisa sobre o Câncer, São Paulo, SP, Brasil. anamaria@compbio.ludwig.org.br
authors.forename: A A
authors.initials: AA
authors.lastname: Camargo
authors.forename: D N
authors.initials: DN
authors.lastname: Nunes
authors.forename: H B
authors.initials: HB
authors.lastname: Samaia
authors.forename: L
authors.initials: L
authors.lastname: Liu
authors.forename: V P
authors.initials: VP
authors.lastname: Collins
authors.forename: A J
authors.initials: AJ
authors.lastname: Simpson
authors.forename: E
authors.initials: E
authors.lastname: Dias-Neto
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002175:Candida / Q000235:genetics; D002897:Chromosomes, Human, Pair 7 / Q000235:genetics*; D017124:Conserved Sequence; D004330:Drosophila / Q000235:genetics; D005784:Gene Amplification; D015870:Gene Expression; D018773:Genes, erbB-1; D005909:Glioblastoma / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D020365:RNA Helicases / Q000737:chemistry* / Q000235:genetics*; D016601:RNA-Binding Proteins; D012269:Ribosomal Proteins; D014407:Tumor Cells, Cultured; D025521:Tumor Suppressor Proteins; D015003:Yeasts / Q000235:genetics
chemical_list: C121427:INTS6 protein, human; D016601:RNA-Binding Proteins; D012269:Ribosomal Proteins; D025521:Tumor Suppressor Proteins; D020365:RNA Helicases