title: Pregnancy-associated glycoproteins (PAG) detection in milk samples for pregnancy diagnosis in dairy goats.
abstract: In ruminants, glycoproteins synthesized in the outer layers of the trophoblast enter the maternal bloodstream. The assay of their concentration in plasma or in serum can be used for pregnancy diagnosis and to indicate feto-placental health status. In this study, concentrations of the pregnancy-associated glycoprotein (PAG) were investigated in the milk of dairy goats. The mean concentrations of PAG in milk were significantly higher in pregnant than in non-pregnant goats throughout the sampling period. Positive milk PAG levels were found in all pregnant animals from Day 32 after breeding, but remained at negative values in nonpregnant ones. This study reports for the first time a pregnancy diagnosis based on milk concentrations of PAG, that could be of great use on the farm.
authors.affiliation: Reproduction and Obstetrics, Faculty of Veterinary, Las Palmas de Gran Canaria, Spain.
authors.forename: F
authors.initials: F
authors.lastname: González
authors.forename: J
authors.initials: J
authors.lastname: Sulon
authors.forename: P
authors.initials: P
authors.lastname: Calero
authors.forename: M
authors.initials: M
authors.lastname: Batista
authors.forename: A
authors.initials: A
authors.lastname: Gracia
authors.forename: J F
authors.initials: JF
authors.lastname: Beckers
mesh_terms: D000818:Animals; D005260:Female; D006023:Glycoproteins / Q000032:analysis / Q000096:biosynthesis*; D006041:Goats / Q000502:physiology*; D008297:Male; D008892:Milk / Q000737:chemistry*; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000032:analysis / Q000096:biosynthesis*; D011259:Pregnancy Tests, Immunologic / Q000379:methods / Q000662:veterinary*; D011863:Radioimmunoassay / Q000662:veterinary
chemical_list: D006023:Glycoproteins; D011257:Pregnancy Proteins