title: Role of flagellum and chemotactic motility of Vibrio anguillarum for phagocytosis by and intracellular survival in fish macrophages.
abstract: The role of the flagellum and chemotactic motility of Vibrio anguillarum for phagocytosis by and intracellular survival in fish macrophages was determined using a wild-type strain, a mutant without the flagellum, a mutant with a truncated flagellum and a non-chemotactic mutant. For all strains, the numbers of intracellular bacteria were relatively low and fell steadily during the observation period. The presence of a flagellum did not influence the uptake by the macrophages, but the smooth swimming phenotype of a non-chemotactic mutant increased its intracellular presence. We suggest that this is due to an increased collision between the mutant and the macrophage, due to a higher average speed of the non-chemotactic mutant.
authors.affiliation: Department of Veterinary Microbiology, The Royal Veterinary and Agricultural University, Stigbøjlen 4, DK-1870 Frederiksberg C, Denmark.
authors.forename: M H
authors.initials: MH
authors.lastname: Larsen
authors.forename: H T
authors.initials: HT
authors.lastname: Boesen
mesh_terms: D000818:Animals; D002633:Chemotaxis / Q000235:genetics / Q000502:physiology; D005393:Fish Diseases / Q000382:microbiology*; D005407:Flagella / Q000235:genetics / Q000502:physiology; D008264:Macrophages / Q000382:microbiology* / Q000502:physiology; D017686:Oncorhynchus mykiss; D010587:Phagocytosis; D014733:Vibrio / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology*; D014735:Vibrio Infections / Q000382:microbiology / Q000662:veterinary*