Distinct ligand binding properties of Mac-2-binding protein and mouse cyclophilin [correction of mousephilin] C-associated protein.
Human Mac-2-binding protein (Mac-2-BP) is a secreted glycoprotein that is widely expressed. It binds to the human macrophage-associated lectin Mac-2 and has been suggested to have a role in host defence. Mouse cyclophilin C-associated protein (mCyCAP) is also a secreted glycoprotein that binds with high affinity to cyclophilin C in the absence of the immunosuppressive drug cyclosporin A. The two proteins share a similar domain structure and considerable sequence identity, including a highly conserved scavenger receptor cysteine-rich domain, and both of them exert their function within the immune system. To elucidate whether these molecules are also functional homologues, we compared their ligand binding properties using cell lines which express Mac-2-BP or mCyCAP as well as transfected cell lines stably expressing mCyCAP or a mutant version lacking the scavenger domain. These experiments show that Mac-2-BP is unable to bind to either human or mouse cyclophilin C and thatmCyCAP cannot bind to Mac-2. The scavenger domain is not required for the interaction between mCyCAP and cyclophilin C. We conclude that these proteins may be part of a larger family of proteins of immunological importance in which closer functional homologues might exists.
Jalkanen
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Centre for Biotechnology, University of Turku, Turku, Finland. sirpa.jalkanen@utu.fi
Leu
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Bono
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Salmi
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Jalkanen
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Smith
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D000818:Animals; D000943:Antigens, Differentiation / Q000378:metabolism*; D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D002352:Carrier Proteins / Q000378:metabolism*; D002460:Cell Line; D000072622:Cyclophilin C; D021983:Cyclophilins / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism; D037502:Galectin 3; D006023:Glycoproteins / Q000378:metabolism*; D006801:Humans; D008024:Ligands; D008565:Membrane Proteins; D051379:Mice; D008970:Molecular Weight; D050778:NFATC Transcription Factors; D009687:Nuclear Proteins; D011971:Receptors, Immunologic / Q000378:metabolism; D018110:Receptors, Lipoprotein; D051116:Receptors, Scavenger; D051122:Scavenger Receptors, Class B; D014157:Transcription Factors / Q000378:metabolism; D016326:Extracellular Matrix Proteins
D000943:Antigens, Differentiation; D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D002352:Carrier Proteins; D000072622:Cyclophilin C; D021983:Cyclophilins; D004268:DNA-Binding Proteins; D037502:Galectin 3; D006023:Glycoproteins; D008024:Ligands; D008565:Membrane Proteins; D050778:NFATC Transcription Factors; D009687:Nuclear Proteins; D011971:Receptors, Immunologic; D018110:Receptors, Lipoprotein; D051116:Receptors, Scavenger; D051122:Scavenger Receptors, Class B; D014157:Transcription Factors; C081856:LGALS3BP protein, human; D016326:Extracellular Matrix Proteins; C082349:Ppicap protein, mouse; C454739:Scarb1 protein, mouse