title: Genomic organization of the X-linked inhibitor of apoptosis and identification of a novel testis-specific transcript.
abstract: Here we report the genomic organization and mapping of the X-linked inhibitor of apoptosis gene (BIRC4, also known as XIAP and hILP) and the identification of a closely related transcript. BIRC4 is located on Xq25 and is composed of seven exons. The intron/exon structure is highly conserved between the mouse homologue and its human counterpart. Four bands cross-react with a BIRC4 coding region probe on a genomic Southern blot. One of these cross-reactive bands encodes an intronless gene that expresses a 2.2-kb transcript solely in the testis. This testis-specific transcript contains a putative open reading frame (ORF) that is homologous to the carboxy-terminal end of BIRC4; overexpression of this ORF shows protective effects against BAX-induced apoptosis.
authors.affiliation: Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, ON, K1H 8M5, Canada.
authors.forename: M
authors.initials: M
authors.lastname: Lagacé
authors.forename: J Y
authors.initials: JY
authors.lastname: Xuan
authors.forename: S S
authors.initials: SS
authors.lastname: Young
authors.forename: C
authors.initials: C
authors.lastname: McRoberts
authors.forename: J
authors.initials: J
authors.lastname: Maier
authors.forename: E
authors.initials: E
authors.lastname: Rajcan-Separovic
authors.forename: R G
authors.initials: RG
authors.lastname: Korneluk
mesh_terms: D000818:Animals; D017209:Apoptosis; D001483:Base Sequence; D015139:Blotting, Southern; D015153:Blotting, Western; D002460:Cell Line; D003001:Cloning, Molecular; D017124:Conserved Sequence; D005091:Exons; D005260:Female; D015723:Gene Library; D006367:HeLa Cells; D006801:Humans; D007438:Introns; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D011506:Proteins / Q000032:analysis / Q000235:genetics*; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000502:physiology; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013737:Testis / Q000378:metabolism; D014162:Transfection; D014960:X Chromosome / Q000235:genetics*; D051636:X-Linked Inhibitor of Apoptosis Protein; D051028:bcl-2-Associated X Protein
chemical_list: C494826:BAX protein, human; C494827:Bax protein, mouse; D011506:Proteins; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; D051636:X-Linked Inhibitor of Apoptosis Protein; C496866:XIAP protein, human; D051028:bcl-2-Associated X Protein