Naturally occurring Clostridium perfringens nontoxic alpha-toxin variant as a potential vaccine candidate against alpha-toxin-associated diseases.
Clostridium perfringens mutant strain 121A/91 shows neither enzymatic (phospholipase C) nor hemolytic activity. Nevertheless, the cpa gene and the corresponding alpha-toxin variant are detectable. Vaccination with this genetically constructed alpha-toxin variant, rAT121/91, induces antibodies capable of significantly reducing activities induced by wild-type toxin. Thus, rAT121/91 could be a useful vaccine candidate.
Schoepe
H
H
Institut für Hygiene und Infektionskrankheiten der Tiere, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany. Heike.Schoepe@vetmed.uni-giessen.de
Pache
C
C
Neubauer
A
A
Potschka
H
H
Schlapp
T
T
Wieler
L H
LH
Baljer
G
G
D019943:Amino Acid Substitution; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology; D000940:Antigenic Variation / Q000235:genetics / Q000276:immunology*; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D001427:Bacterial Toxins / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D001428:Bacterial Vaccines / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D002135:Calcium-Binding Proteins; D003015:Clostridium Infections / Q000517:prevention & control; D003016:Clostridium perfringens / Q000201:enzymology / Q000235:genetics / Q000276:immunology*; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D016296:Mutagenesis; D010738:Type C Phospholipases / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D014614:Vaccines, Synthetic / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D001427:Bacterial Toxins; D001428:Bacterial Vaccines; D002135:Calcium-Binding Proteins; D014614:Vaccines, Synthetic; D010738:Type C Phospholipases; C040649:alpha toxin, Clostridium perfringens