[A preliminary study of loss of heterozygosity on chromosome 14 in glioblastoma].
OBJECTIVE
In order to locate the deletion areas probably harboring tumor suppressor genes on chromosome 14 and provide clues for discovering novel tumor suppressor genes.


METHODS
Fourteen loci on chromosome 14 were examined to detect loss of heterozygosity(LOH) in 20 cases of glioblastoma(GBM) by PCR based microsatellite polymorphism analyses, in which fluorescence-labeled primers and Perkin Elmer 377 DNA Sequencer were applied.


RESULTS
50% informative cases of GBM displayed LOH on chromosome 14; 38.2% of informative loci showed LOH in our series, in which the most frequent LOH was observed at locus D14S65(57.1%) on 14q31-32.3 and in the chromosomal region from locus D14S63 (46.7%) to D14S74(47.1%) on 14q21-24.1. 32% of informative cases displayed LOH on 14p and 50% on 14q. No microsatellite instability was observed.


CONCLUSIONS
Loss of genetic material on chromosome 14q may play an important role in molecular genetic pathogenesis of GBM. The chromosomal regions at D14S65 on 14q31-32.3 and from D14S63 to D14S74 on 14q21-24.1 may harbor novel tumor suppressor genes associated with GBM.
Hu
J
J
Department of Neurosurgery, Hua Shan Hospital, Medical Center of Fudan University, Shanghai, 200040 P.R. China. ly045012@online.sh.cn
Jiang
C
C
Ng
H K
HK
Pang
J C
JC
Tong
C Y
CY
D001932:Brain Neoplasms / Q000235:genetics*; D002883:Chromosomes, Human, Pair 14; D005909:Glioblastoma / Q000235:genetics*; D006801:Humans; D019656:Loss of Heterozygosity; D018895:Microsatellite Repeats