[Effects of Tremella polysaccharide on cytoplasmic free calcium concentration in murine splenocytes].
Effects of Tremella polysaccharides (TP) isolated from Tremella fuciformis Berk. on the cytoplasmic free calcium concentration in murine splenocytes were observed using Fura-2, a fluorescence indicator, technique. TP increased the cytoplasmic free calcium concentration of Fura-2-loaded murine splenocytes in a concentration-dependent manner and showed synergetic effect with Con A. No effect was found in the release of intracellular calcium without extracellular calcium. Verapamil (10 micrograms.ml-1), a calcium channel blocker, was shown to inhibit the effect of TP on cytoplasmic free calcium, indicating that TP can affect the influx of extracellular calcium.
Cui
J Y
JY
Department of Pharmacology, Beijing Medical University, Beijing 100083.
Lin
Z B
ZB
D000818:Animals; D001487:Basidiomycota / Q000737:chemistry; D001693:Biological Transport, Active; D002118:Calcium / Q000378:metabolism*; D002469:Cell Separation; D003593:Cytoplasm / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005260:Female; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology / Q000378:metabolism*
D011134:Polysaccharides; D002118:Calcium