title: Immunohistochemical analysis of the role of angiogenic status in the vasculature of peritoneal endometriosis.
abstract: OBJECTIVE
  To investigate the angiogenic status of the vasculature in peritoneal endometriosis based on macroscopic appearance.
  
  
  METHODS
  Prospective study.
  
  
  METHODS
  University hospital.
  
  
  METHODS
  Patients with peritoneal endometriosis.
  
  
  METHODS
  During laparoscopy, 25 samples of predominantly red peritoneal lesions and 27 samples of predominantly black peritoneal lesions were collected from a total of 31 patients with endometriosis. Eutopic endometrium from 25 patients with endometriosis was collected by curettage during laparoscopy or just after surgery.
  
  
  METHODS
  A proliferating endothelial cell index (PCI) was determined by calculating the percentage of microvessels that contained proliferating endothelial cells. A vessel maturation index (VMI) was determined by calculating the percentage of microvessels showing colocalization of CD34- and alpha-SMA-positive staining.
  
  
  RESULTS
  Peritoneal endometriotic tissues had extremely low or null PCI. The VMI of red peritoneal lesions was significantly lower than that of black ones. Vessel maturation index of red lesions was higher that that of proliferative eutopic endometrium and similar to that of secretory eutopic endometrium.
  
  
  CONCLUSIONS
  Compared with the case of black peritoneal lesions, red lesions had a much higher fraction of immature vessels.
authors.affiliation: Department of Obstetrics and Gynecology, Tohoku University School of Medicine, Sendai, Japan. sachikoMA@aol.com
authors.forename: S
authors.initials: S
authors.lastname: Matsuzaki
authors.forename: M
authors.initials: M
authors.lastname: Canis
authors.forename: T
authors.initials: T
authors.lastname: Murakami
authors.forename: P
authors.initials: P
authors.lastname: Dechelotte
authors.forename: M A
authors.initials: MA
authors.lastname: Bruhat
authors.forename: K
authors.initials: K
authors.lastname: Okamura
mesh_terms: D000199:Actins / Q000378:metabolism; D000328:Adult; D018952:Antigens, CD34 / Q000378:metabolism; D001808:Blood Vessels / Q000378:metabolism / Q000473:pathology; D002455:Cell Division; D004715:Endometriosis / Q000150:complications*; D004730:Endothelium, Vascular / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D009131:Muscle, Smooth, Vascular / Q000378:metabolism; D009389:Neovascularization, Pathologic / Q000209:etiology* / Q000473:pathology; D010532:Peritoneal Diseases / Q000150:complications*; D011247:Pregnancy; D011446:Prospective Studies
chemical_list: D000199:Actins; D018952:Antigens, CD34