Uptake of D-serine by synaptosomal P2 fraction isolated from rat brain.
We have demonstrated that the crude synaptosome P2 fraction prepared from the rat brain is able to accumulate [(3)H]D-serine in a saturable, temperature-dependent and partially sodium- and potassium-dependent manner with an affinity of a hundred micromolar range. The inhibition profile of D-serine accumulation by various amino acids is different from those of uptake systems reported for glycine and other amino acids. The present data suggest that the endogenous D-serine may be taken up mainly through a carrier-mediated transport system to regulate its extracellular concentration in the mammalian brain.
Yamamoto
N
N
Department of Mental Disorder Research, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Tokyo 187-8502 Japan.
Tomita
U
U
Umino
A
A
Nishikawa
T
T
D000409:Alanine / Q000378:metabolism; D000818:Animals; D001667:Binding, Competitive / Q000187:drug effects / Q000502:physiology; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D002352:Carrier Proteins / Q000187:drug effects / Q000378:metabolism*; D005110:Extracellular Space / Q000187:drug effects / Q000378:metabolism*; D018698:Glutamic Acid / Q000378:metabolism; D005998:Glycine / Q000378:metabolism; D007536:Isomerism; D008297:Male; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D018377:Neurotransmitter Agents / Q000378:metabolism; D010599:Pharmacokinetics; D051381:Rats; D017208:Rats, Wistar; D012694:Serine / Q000378:metabolism*; D013574:Synaptosomes / Q000187:drug effects / Q000378:metabolism*; D013696:Temperature; D014316:Tritium; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
D002352:Carrier Proteins; D018377:Neurotransmitter Agents; D014316:Tritium; D018698:Glutamic Acid; D012694:Serine; D005680:gamma-Aminobutyric Acid; D000409:Alanine; D005998:Glycine