Identification of a group 1-like capsular polysaccharide operon for Vibrio vulnificus.
Virulence of Vibrio vulnificus correlates with changes in colony morphology that are indicative of a reversible phase variation for expression of capsular polysaccharide (CPS). Encapsulated variants are virulent with opaque colonies, whereas phase variants with reduced CPS expression are attenuated and are translucent. Using TnphoA mutagenesis, we identified a V. vulnificus CPS locus, which included an upstream ops element, a wza gene (wza(Vv)), and several open reading frames with homology to CPS biosynthetic genes. This genetic organization is characteristic of group 1 CPS operons. The wza gene product is required for transport of CPS to the cell surface in Escherichia coli. Polar transposon mutations in wza(Vv) eliminated expression of downstream biosynthetic genes, confirming operon structure. On the other hand, nonpolar inactivation of wza(Vv) was specific for CPS transport, did not alter CPS biosynthesis, and could be complemented in trans. Southern analysis of CPS phase variants revealed deletions or rearrangements at this locus. A survey of environmental isolates indicated a correlation between deletions in wza(Vv) and loss of virulent phenotype, suggesting a genetic mechanism for CPS phase variation. Full virulence in mice required surface expression of CPS and supported the essential role of capsule in the pathogenesis of V. vulnificus.
Wright
A C
AC
Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA. awright@gnv.ifas.ufl.edu
Powell
J L
JL
Kaper
J B
JB
Morris
J G
JG
D000595:Amino Acid Sequence; D000818:Animals; D016667:Bacterial Capsules / Q000235:genetics*; D001483:Base Sequence; D001692:Biological Transport; D002462:Cell Membrane / Q000378:metabolism; D017124:Conserved Sequence; D004269:DNA, Bacterial; D004195:Disease Models, Animal; D005260:Female; D014644:Genetic Variation; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009876:Operon; D012045:Regulatory Sequences, Nucleic Acid; D017386:Sequence Homology, Amino Acid; D014733:Vibrio / Q000235:genetics* / Q000472:pathogenicity; D014735:Vibrio Infections / Q000382:microbiology
D004269:DNA, Bacterial