title: Mitochondrial perturbations and oxidant stress in lymphocytes from patients undergoing surgery and general anesthesia.
abstract: BACKGROUND
  Previous studies have shown that a profound suppression of immune function transiently occurs in patients who undergo surgery under general anesthesia. The decline in the absolute counts of peripheral blood lymphocytes constitutes a major factor accounting for this immune defect, and recent evidence indicates that apoptosis plays a crucial role in determining postsurgical lymphocytopenia.
  
  
  OBJECTIVE
  An altered oxidation-reduction status of mitochondria may contribute through apoptosis to the loss of lymphocytes following surgical trauma and general anesthesia.
  
  
  METHODS
  We studied 16 patients with American Society of Anesthesiologists' physical status I or II who underwent elective surgery under general anesthesia. The data were collected prospectively.
  
  
  METHODS
  University hospital.
  
  
  METHODS
  Samples of peripheral blood were drawn on the day before surgery and at 24 and 96 hours after the operation. Following lymphocyte isolation, the mitochondrial transmembrane potential was assessed by flow cytometry using 3,3'-dihexylocarbo-cyanine iodide, and stains with hydroethidine and 2'-7'-dichlorofluorescein diacetate were used to determine the generation of reactive oxygen species. The labeling of lymphocytes with monobromobimane was used to assess the presence of reduced glutathione.
  
  
  RESULTS
  At 24 hours after surgery, we detected a significantly elevated frequency of peripheral blood lymphocytes (P =.002), which incorporated low levels of 3,3'-dihexylocarbo-cyanine iodide, compared with the preoperative period. At this same time point, the frequency of lymphocytes with the hydroethidine- and 2'-7'-dichlorofluorescein diacetate-positive phenotype was elevated compared with baseline levels. Conversely, at 24 hours after surgery, the frequency of cells that stained positive for glutathione was strongly decreased compared with preoperative values. Overall measurements returned to the baseline levels at 96 hours after surgery.
  
  
  CONCLUSIONS
  The strict association we observed between the overproduction of reactive oxygen species and the disruption of the mitochondrial transmembrane potential supports the view that alterations in mitochondrial energy metabolism, paralleled by the presence of a pro-oxidant oxidation-reduction status, could be involved in the accelerated apoptotic loss of lymphocytes following surgical trauma and general anesthesia.
authors.affiliation: Institute of Anesthesia and Intensive Care, Policlinico Umberto I, "La Sapienza" University, 00161 Rome, Italy. giovanna.delogu@uniroma1.it
authors.forename: G
authors.initials: G
authors.lastname: Delogu
authors.forename: S
authors.initials: S
authors.lastname: Moretti
authors.forename: G
authors.initials: G
authors.lastname: Famularo
authors.forename: S
authors.initials: S
authors.lastname: Marcellini
authors.forename: G
authors.initials: G
authors.lastname: Santini
authors.forename: A
authors.initials: A
authors.lastname: Antonucci
authors.forename: M
authors.initials: M
authors.lastname: Marandola
authors.forename: L
authors.initials: L
authors.lastname: Signore
mesh_terms: D000768:Anesthesia, General; D017209:Apoptosis; D005260:Female; D005434:Flow Cytometry; D005978:Glutathione / Q000378:metabolism; D006651:Histocytochemistry; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism* / Q000648:ultrastructure; D008297:Male; D008564:Membrane Potentials; D008875:Middle Aged; D008928:Mitochondria / Q000378:metabolism* / Q000502:physiology; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D011446:Prospective Studies; D017382:Reactive Oxygen Species / Q000378:metabolism; D013514:Surgical Procedures, Operative
chemical_list: D017382:Reactive Oxygen Species; D005978:Glutathione