title: The impact of codon 54 variation in intestinal fatty acid binding protein gene on the pathogenesis of diabetes mellitus in Chinese.
abstract: OBJECTIVE
  To investigate whether or not the intestinal fatty acid binding protein gene (FABP2)-Ala54Thr variation is related to non-insulin dependent diabetes mellitus (NIDDM), obesity, dyslipidemia and glucose stimulated insulin secretion (GSIS) in Chinese.
  
  
  METHODS
  The FABP2-Ala54Thr variation was detected by PCR/Hhal digestion in 231 Chinese subjects (116 with normal glucose tolerance (NGT), 54 with impaired glucose tolerance (IGT) and 61 with NIDDM). Plasma glucose, insulin and C-peptide levels before and after 75 g glucose load as well as fasting lipid profile were determined.
  
  
  RESULTS
  (1) The Ala54 and Thr54 allele frequencies in Chinese were 0.71 and 0.29 respectively; (2) The FABP2-Ala54Thr variation was neither associated with fasting and post-challenged plasma glucose levels nor with NIDDM; (3) This variation was neither associated with fasting lipid profile nor with obesity; (4) The IGT subjects with genotype Thr54(+) (Thr54 homozygotes and heterozygotes) had lower fasting, 2-hour and total C-peptide levels and smaller AUC representing lesser C-peptide secretion after glucose challenge than those with genotype Thr54(-) (Ala54 homozygotes) (P = 0.04, 0.03, 0.01 and 0.01 respectively). The serum insulin levels changed in the same tendency.
  
  
  CONCLUSIONS
  The glucose stimulated insulin secretion (GSIS) reserve of islet beta-cells is more limited in subjects with FABP2-Thr54(+) genotype than in those with FABP2-Thr54(-) genotype. It suggests that FABP2-codon 54 variation might contribute to the insufficient insulin secretion in the development of NIDDM in Chinese.
authors.affiliation: Department of Endocrinology and Metabolism, Diabetes Research Laboratory, Medical Genetics Research Laboratory, Shanghai Sixth People Hospital, Shanghai 200233, China.
authors.forename: K
authors.initials: K
authors.lastname: Xiang
authors.forename: T
authors.initials: T
authors.lastname: Zheng
authors.forename: W
authors.initials: W
authors.lastname: Jia
authors.forename: D
authors.initials: D
authors.lastname: Sun
authors.forename: W
authors.initials: W
authors.lastname: Ding
authors.forename: J
authors.initials: J
authors.lastname: Lu
authors.forename: J
authors.initials: J
authors.lastname: Tang
mesh_terms: D000409:Alanine / Q000235:genetics; D044466:Asian People; D002352:Carrier Proteins / Q000235:genetics*; D002681:China; D003062:Codon; D003924:Diabetes Mellitus, Type 2 / Q000209:etiology / Q000235:genetics*; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D014644:Genetic Variation; D006801:Humans; D007328:Insulin / Q000378:metabolism; D007333:Insulin Resistance / Q000235:genetics*; D000078790:Insulin Secretion; D009363:Neoplasm Proteins; D013912:Threonine / Q000235:genetics; D025521:Tumor Suppressor Proteins
chemical_list: D002352:Carrier Proteins; D003062:Codon; C493404:FABP2 protein, human; C107608:FABP7 protein, human; C493405:Fabp2 protein, mouse; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D007328:Insulin; D009363:Neoplasm Proteins; D025521:Tumor Suppressor Proteins; D013912:Threonine; D000409:Alanine