title: Cell culture test system for express analysis of cytotoxic and growth-stimulating effects of bioactive compounds.
abstract: Cultures of human and mammalian cells presenting 4 types of differentiation (normal human fibroblasts and myoblasts, human and Syrian hamster hepatoma cells, and mouse/mouse hybridoma cells) were used in a panel biotest system. This system allowed to evaluate the cytotoxic and stimulatory effect of bioactive compounds by determining the dose-effect relationships and some quantitative parameters including LD(50). Examination of some biolactive compounds of different nature (sangviritrin, escin, deltostim, cycloheximide, dexamethasone) confirmed high efficacy of this biotest system.
authors.affiliation: Medical Genetic Research Center, Russian Academy of Medical Sciences; Research Center of Biomedical Technologies, Moscow.
authors.forename: V G
authors.initials: VG
authors.lastname: Chernikov
authors.forename: S M
authors.initials: SM
authors.lastname: Terekhov
authors.forename: T B
authors.initials: TB
authors.lastname: Krokhina
authors.forename: S S
authors.initials: SS
authors.lastname: Shishkin
authors.forename: T D
authors.initials: TD
authors.lastname: Smirnova
authors.forename: N V
authors.initials: NV
authors.lastname: Adnoral
authors.forename: L B
authors.initials: LB
authors.lastname: Rebrov
authors.forename: Y I
authors.initials: YI
authors.lastname: Denisov-Nikol'skii
authors.forename: B A
authors.initials: BA
authors.lastname: Bykov
mesh_terms: D000818:Animals; D001681:Biological Assay; D018929:Cell Culture Techniques; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D006224:Cricetinae; D016903:Drug Monitoring / Q000379:methods*; D006801:Humans; D051379:Mice