Two distinct isopentenyl diphosphate isomerases in cytosol and plastid are differentially induced by environmental stresses in tobacco.
Two distinct cDNA clones (IPI1 and IPI2) encoding IPI were isolated from Nicotiana tabacum. In situ expression of isopentenyl diphosphate isomerase-1 (IPI1)- and IPI2-green fluorescent protein fusion constructs revealed that IPI1 and IPI2 were localized in chloroplast and cytosol, respectively. The level of IPI1 mRNA was increased under high-salt and high-light stress conditions, while that of IPI2 mRNA was increased under high-salt and cold stress conditions. Both IPI transcripts were increased in an abscisic acid-independent manner. This is the first report of a cytosolic IPI. The results indicated that two distinct IPIs were differentially induced in response to stress.
Nakamura
A
A
Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Midori-ku, Yokohama 226-8501, Japan.
Shimada
H
H
Masuda
T
T
Ohta
H
H
Takamiya
K
K
D000040:Abscisic Acid / Q000494:pharmacology; D001483:Base Sequence; D019748:Carbon-Carbon Double Bond Isomerases / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D003600:Cytosol / Q000201:enzymology*; D017931:DNA Primers; D049452:Green Fluorescent Proteins; D045782:Hemiterpenes; D007527:Isoenzymes / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D008164:Luminescent Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D010947:Plants, Toxic; D018087:Plastids / Q000201:enzymology*; D011993:Recombinant Fusion Proteins / Q000235:genetics; D013347:Subcellular Fractions / Q000201:enzymology; D014026:Nicotiana / Q000201:enzymology*
D017931:DNA Primers; D045782:Hemiterpenes; D007527:Isoenzymes; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; D000040:Abscisic Acid; D019748:Carbon-Carbon Double Bond Isomerases; C021453:isopentenyldiphosphate delta-isomerase