title: [Detection of Cre recombinase activity in Mx-Cre transgenic mice induced by INF in vitro].
abstract: The Cre recombinase and its activity in C57-TgN(Mx-Cre) transgenic mice is studied by polymerase chain reaction (PCR), Western blot, immunohistochemistry, immunogold electron microscopy and Southern blot. C57-TgN(Mx-Cre) transgenic mice harbouring cre gene in genomic DNA is demonstrated by PCR, and these mice which are induced by INF-alpha 1b could express Cre recombinase, which is confirmed by Western blot. With immunohistochemistry, we find that the Cre recombinase expresses in hepatocyte cytoplasm and nuclear of C57-TgN(Mx-Cre) transgenic mice. Cre recombinase expressed in hepatocyte cytoplasm and nuclear is further confirmed by immunogold electon microscopy. And it is supported that the Cre recombinase which is created from C57-TgN(Mx-Cre) transgenic mice induced by INF-alpha 1b can direct DNA recombination reaction in vitro. All evidence leads us supporting the view that the Cre recombinase expressed in C57-TgN(Mx-Cre) transgenic mice has activity. Thus we find a method to detect the activity of Cre recombinase in vitro.
authors.affiliation: Department of Cell Biology, Second Military Medical University, Shanghai 200433, China.
authors.forename: X Y
authors.initials: XY
authors.lastname: Zi
authors.forename: Y C
authors.initials: YC
authors.lastname: Yao
authors.forename: J
authors.initials: J
authors.lastname: Xiong
authors.forename: J X
authors.initials: JX
authors.lastname: Li
authors.forename: Y B
authors.initials: YB
authors.lastname: Yan
authors.forename: H Y
authors.initials: HY
authors.lastname: Yu
authors.forename: Y P
authors.initials: YP
authors.lastname: Hu
mesh_terms: D000818:Animals; D004790:Enzyme Induction; D019204:GTP-Binding Proteins; D019426:Integrases / Q000378:metabolism*; D007372:Interferons / Q000494:pharmacology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D064547:Myxovirus Resistance Proteins; D016133:Polymerase Chain Reaction; D011506:Proteins / Q000502:physiology*; D014764:Viral Proteins / Q000378:metabolism*
chemical_list: C579070:Mx1 protein, mouse; D064547:Myxovirus Resistance Proteins; D011506:Proteins; D014764:Viral Proteins; D007372:Interferons; C045073:Cre recombinase; D019426:Integrases; D019204:GTP-Binding Proteins