Electrophoresis of creatine kinase isoforms: a highly sensitive fluorescence scanning method.
OBJECTIVE
To develop an agarose electrophoretic method for creatine kinase (CK) isoforms, using highly sensitive fluorescence scanning.


METHODS
A discontinuous buffer system was used. Electrophoresis on agarose gel was performed under constant current and low voltage. CK isoforms were separated within 30 minutes and detected by fluorescence scanning.


RESULTS
There were no significant differences when the activities of CK-MM were between 853.0 U/L and 14.0 U/L and those of CK-MB between 152.0 U/L and 2.4 U/L. The detection limits of stain method for CK-MM and CK-MB isoforms were 36.0 U/L and 12.3 U/L, respectively; while those of fluorescence method were 12.0 U/L and 2.1 U/L. The experimental results showed good precision for CK-MM isoforms, as well for CK-MB isoforms and isoenzymes.


CONCLUSIONS
An agarose electrophoretic method has been developed to measure CK isoenzymes and isoforms clinically. This method is rapid, simple, sensitive, highly reproducible and inexpensive. It is suitable for general laboratories.
Zhao
X
X
Department of Laboratory Medicine, Beijing Hospital, Beijing 100730, China.
Bi
C
C
Yang
Z
Z
D003402:Creatine Kinase / Q000032:analysis / Q000378:metabolism*; D052279:Creatine Kinase, MB Form; D052283:Creatine Kinase, MM Form; D004587:Electrophoresis, Agar Gel; D005456:Fluorescent Dyes; D006801:Humans; D007527:Isoenzymes / Q000032:analysis / Q000378:metabolism*; D012680:Sensitivity and Specificity
D005456:Fluorescent Dyes; D007527:Isoenzymes; D003402:Creatine Kinase; D052279:Creatine Kinase, MB Form; D052283:Creatine Kinase, MM Form