Sd(a)-antigen-like structures carried on core 3 are prominent features of glycans from the mucin of normal human descending colon.
This paper describes structural characterization by NMR, MS and degradative studies of mucin glycans from normal human descending colon obtained freshly at autopsy. The saccharides were mainly based on core 3 (GlcNAcbeta1-3GalNAc). Among the terminal saccharide determinants Sd(a)/Cad-antigen-like structures were prominent, and Lewis x, sialyl Lewis x and sulphated Lewis x were found as minor components, whereas blood group H and A antigenic determinants were absent. The saccharides were markedly different from those of mucins from colon cancers or colon cancer cell lines analysed so far, in which cores 1 and 2 are prominent features, and in which various other terminal determinants have been found, but not Sd(a)/Cad.
Capon
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Laboratoire de Glycobiologie structurale et fonctionnelle, Unité Mixte du Centre National de la Recherche Scientifique, Université des Sciences et Technologies de Lille, Villeneuve d'Ascq Cedex, France.
Maes
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E
Michalski
J C
JC
Leffler
H
H
Kim
Y S
YS
D001344:Autopsy; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D003106:Colon / Q000737:chemistry*; D006649:Histocompatibility Antigens / Q000737:chemistry; D006801:Humans; D007413:Intestinal Mucosa / Q000737:chemistry*; D016256:Lewis X Antigen / Q000737:chemistry / Q000031:analogs & derivatives; D009077:Mucins / Q000737:chemistry* / Q000302:isolation & purification; D019906:Nuclear Magnetic Resonance, Biomolecular; D009844:Oligosaccharides / Q000737:chemistry* / Q000302:isolation & purification; D011134:Polysaccharides / Q000737:chemistry; D000080506:Sialyl Lewis X Antigen; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
C402829:3-sulfated Lewis x; D006649:Histocompatibility Antigens; D016256:Lewis X Antigen; D009077:Mucins; D009844:Oligosaccharides; D011134:Polysaccharides; C106678:Sd(a) determinant; D000080506:Sialyl Lewis X Antigen