Multiple effects of chromate on the photosynthetic apparatus of Spirodela polyrhiza as probed by OJIP chlorophyll a fluorescence measurements.
Chromate (Cr) decreases the growth of Spirodela polyrhiza. The fronds lost their pigments. The O2 evolution was also decreased. The Cr effect was found to be dose dependent. The toxic effects of Cr have further been studied on the photosynthetic activity of Spirodela polyrhiza by means of the chlorophyll a (Chl a) fluorescence transient O-J-I-P. The Chl a fluorescence transients were recorded in vivo with high time resolution and analyzed according to the JIP-test which can quantify the photosystem II behavior. Cr treated plants show a decrease in yield for primary photochemistry, phi Po. The performance index of PSII, PIABS, which is the combination of the indexes of three independent parameters, (1) the total number of active reaction centers per absorption (RC/ABS), (2) yield of primary photochemistry (phi Po) and (3) efficiency with which a trapped exciton can move an electron into the electron transport chain (psi 0), decreased due to Cr treatment. Chromate sensitivity varies within plant populations. In summary Cr affects several targets of PSII. More specifically, the main targets of Cr, according to the JIP-test, can be listed as a decrease in the number of active reaction centers and damage to the oxygen-evolving complex.
Appenroth
K J
KJ
Department of Plant Physiology, University of Jena, Dornburger Str. 159, D-07743 Jena, Germany.
Stöckel
J
J
Srivastava
A
A
Strasser
R J
RJ
D029064:Araceae / Q000502:physiology*; D015415:Biomarkers / Q000032:analysis; D002734:Chlorophyll / Q000032:analysis*; D000077194:Chlorophyll A; D002840:Chromates / Q000009:adverse effects*; D005453:Fluorescence; D010100:Oxygen / Q000378:metabolism; D010788:Photosynthesis / Q000187:drug effects*; D014874:Water Pollutants, Chemical / Q000009:adverse effects*
D015415:Biomarkers; D002840:Chromates; D014874:Water Pollutants, Chemical; D002734:Chlorophyll; D010100:Oxygen; D000077194:Chlorophyll A