A human immunodeficiency virus Env inducible transcription system to examine consequences of gp120 expression.
According to several studies, the HIV-1 envelope gp120 protein and the co-receptor CXCR4 play an essential role in HIV-1 induced cell toxicity. Characterisation of the CD4-independent m7NDK isolate provided the opportunity of studying the effects of direct interactions between m7NDK gp120 and CXCR4. Therefore, an inducible expression system was designed enabling synthesis of HIV-1 Env proteins upon doxycycline induction. Analysis of the expression of the env gene of the m7NDK HIV-1 isolate revealed, unexpectedly, that even long-term expression of m7NDK gp120 did not result in cytotoxycity in CXCR4-positive or -negative cell lines. This is the first report of a CD4-independent HIV-1-protein inducible expression regulated through the Tet-On system and by an alternative splicing. Env inducible expression cell lines could constitute a useful cellular tool to undertake analysis of HIV Env protein expression.
Joliot
V
V
INSERM Unité 380, Laboratoire de Génétique et Pathologie Expérimentales, Institut Cochin de Génétique Moléculaire, 22 Rue Méchain, 75014, Paris, France.
Goujon
C
C
Dumonceaux
J
J
Renard
A
A
Briand
P
P
Hazan
U
U
D001253:Astrocytes / Q000166:cytology; D015152:Blotting, Northern; D015153:Blotting, Western; D015704:CD4 Antigens / Q000378:metabolism*; D002459:Cell Fusion; D002460:Cell Line; D018920:Coculture Techniques; D015967:Gene Expression Regulation, Viral; D017930:Genes, Reporter; D005822:Genetic Vectors; D015699:HIV Envelope Protein gp120 / Q000096:biosynthesis* / Q000378:metabolism*; D015497:HIV-1 / Q000235:genetics / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D007700:Kinetics; D019718:Receptors, CXCR4 / Q000378:metabolism*; D014158:Transcription, Genetic; D014162:Transfection
D015704:CD4 Antigens; D015699:HIV Envelope Protein gp120; D019718:Receptors, CXCR4