Regulation of water channel gene (AQP-CHIP) expression by estradiol and anordiol in rat uterus.
In the present studies, we observed the regulation of water channel gene (AQP-CHIP) expression by estradiol (E2) and anordiol, an antiestrogen with agonist activity, in immature female rat uterus. Antisense and sense oligonucleotide primers corresponding to the consensus sequences of two rats AQP-CHIP water channels were synthesized and used to amplify a cDNA fragment that was reverse-transcripted from rat uterine total RNA preparation. E2 administered as a single dose of 40 micrograms.kg-1 to immature female rats induced a significant increase in AQP-CHIP mRNA expression 9 h after treatment. The lowest effective doses of E2 and anordiol were 40 and 50 micrograms.kg-1, respectively. The stimulatory effect of anordiol was more pronounced than that of E2. The present results suggest that AQP-CHIP water channel gene expression may be involved in E2- and anordiol-mediated water imbibition and luminal fluid production in the uterus.
Li
X J
XJ
Department of Pharmacology, Beijing Medical University, Beijing 100083.
Yu
H M
HM
Koide
S S
SS
D000818:Animals; D051398:Aquaporin 1; D020346:Aquaporins / Q000096:biosynthesis* / Q000235:genetics; D003281:Contraceptives, Postcoital / Q000494:pharmacology; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D015870:Gene Expression; D009635:Norandrostanes / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D014599:Uterus / Q000378:metabolism*
C496022:Aqp1 protein, rat; D020346:Aquaporins; D003281:Contraceptives, Postcoital; D009635:Norandrostanes; D012333:RNA, Messenger; D051398:Aquaporin 1; D004958:Estradiol; C023615:anordiol