title: Completion of the nucleotide sequence of the Enterococcus faecalis conjugative virulence plasmid pAD1 and identification of a second transfer origin.
abstract: pAD1 is a 59.3-kb plasmid in Enterococcus faecalis that has been the subject of intense investigation with regard to its pheromone-inducible conjugation behavior as well as its contribution to virulence. Approximately two-thirds of the pAD1 nucleotide sequence has been previously reported. Here we report on an analysis of the final approximately 22 kb, a significant portion of which is believed to encode structural genes associated with conjugation. The conjugation-related region was also found to contain a new (second) origin of conjugative transfer (oriT). A list of open reading frames covering the entire plasmid is presented.
authors.affiliation: Department of Biologic and Materials Sciences, School of Dentistry, University of Michigan, Ann Arbor, MI 48109, USA.
authors.forename: M V
authors.initials: MV
authors.lastname: Francia
authors.forename: W
authors.initials: W
authors.lastname: Haas
authors.forename: R
authors.initials: R
authors.lastname: Wirth
authors.forename: E
authors.initials: E
authors.lastname: Samberger
authors.forename: A
authors.initials: A
authors.lastname: Muscholl-Silberhorn
authors.forename: M S
authors.initials: MS
authors.lastname: Gilmore
authors.forename: Y
authors.initials: Y
authors.lastname: Ike
authors.forename: K E
authors.initials: KE
authors.lastname: Weaver
authors.forename: F Y
authors.initials: FY
authors.lastname: An
authors.forename: D B
authors.initials: DB
authors.lastname: Clewell
mesh_terms: D003227:Conjugation, Genetic / Q000235:genetics*; D015246:Deoxyribonuclease EcoRI / Q000378:metabolism; D013293:Enterococcus faecalis / Q000235:genetics* / Q000472:pathogenicity*; D005798:Genes, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D010957:Plasmids / Q000235:genetics*; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
chemical_list: D015246:Deoxyribonuclease EcoRI