Oligonucleotides containing a lysine residue as 3'-3' junction for alternate strand triple helix formation.
Oligonucleotides with a 3'-3' inversion of polarity site assured by one lysine residue have been synthesized, characterized and used as third strands in alternate strand triple helix formation. UV melting studies and molecular mechanics calculations have been carried out to investigate the stability and the geometry of these new triplexes.
Barone
G
G
Dipartimento di Chimica, Università di Napoli "Federico II", Via Cinthia, 4, Complesso Universitario di Monte S. Angelo, I-80126, Napoli, Italy.
De Napoli
L
L
Di Fabio
G
G
Giancola
C
C
Messere
A
A
Montesarchio
D
D
Petraccone
L
L
Piccialli
G
G
D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D004355:Drug Stability; D008239:Lysine / Q000737:chemistry*; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000737:chemistry*; D013057:Spectrum Analysis; D013696:Temperature
D009841:Oligonucleotides; C051802:triplex DNA; D004247:DNA; D008239:Lysine