[The effects on melanoma and immunological mechanism of human interferon-alpha gene therapy by fibroblasts].
OBJECTIVE
To establish a model of fibroblast cell-mediated human IFN-alpha gene therapy for anti-melanoma.


METHODS
BMGNeo-IFN-alpha DNA was transferred into NIH3T3 fibroblasts by the liposome method. The clone secreting the highest level of IFN-alpha was selected and implanted to mice intraperitoneally.


RESULTS
From 12 hours after implantation, IFN-alpha activity could be detected in serum of the animal and was sustained for a long time. We used the model to treat the nude mice inoculated with melanoma and found that the growth of M21 cells was inhibited obviously.


CONCLUSIONS
The results demonstrated that fibroblasts could successfully transfer and express hIFN-alpha gene in vivo and the fibroblast cell-mediated gene therapy could be used to treat human melanoma.
Chen
M
M
Department of Plastic and Reconstructive Surgery, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China.
Lin
Z
Z
Cao
X
X
D016475:3T3 Cells; D000818:Animals; D005347:Fibroblasts / Q000378:metabolism*; D015316:Genetic Therapy; D006801:Humans; D016898:Interferon-alpha / Q000235:genetics* / Q000276:immunology; D008297:Male; D008545:Melanoma / Q000276:immunology* / Q000473:pathology / Q000628:therapy*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D014407:Tumor Cells, Cultured
D016898:Interferon-alpha