title: Presence of oxytocinergic neuronal-like cells in the bovine pineal gland: an immunocytochemical and in situ hybridization study.
abstract: In the last decade, there is more and more evidence showing the role of the central innervation of the pineal gland, but there are controversies around the intra or extrapineal origin of oxytocin found within the pineal tissue. In order to check the amount and the site of synthesis of oxytocin in the bovine pineal gland, we performed a morphological and chromatographic study. The anatomical distribution of the pineal oxytocin was explored by immunohistochemistry and in situ hybridization for the corresponding mRNA. The results confirm the presence of oxytocinergic fibres in the bovine pineal, some of them endowed with big varicosities. Immunohistochemistry also displayed neuronal-like cells in the pineal body. The in situ hybridization for the mRNA encoding pre-pro/oxytocin-NFZ I used a mixture of three oligonucleotide probes labelled with (35)S. This allowed identification of positive cells in the bovine pineal. The content in oxytocin was evaluated by radioimmunoassay during 5 months, from July to November, and the peptidic extract revealed an increase of pineal oxytocin immunoreactivity in September as compared with July or November. The significance of intrinsic oxytocin innervation of the bovine pineal gland, as well as the threefold increase of the oxytocin content in the pineal in September, remains to be elucidated.
authors.affiliation: Department of Endocrinology II, Carol Davila University of Medicine and Pharmacy, PO Box 41-31, 78260 Bucharest, Romania. rpnes@sunu.mc.ro
authors.forename: C
authors.initials: C
authors.lastname: Badiu
authors.forename: L
authors.initials: L
authors.lastname: Badiu
authors.forename: M
authors.initials: M
authors.lastname: Coculescu
authors.forename: H
authors.initials: H
authors.lastname: Vilhardt
authors.forename: M
authors.initials: M
authors.lastname: Møller
mesh_terms: D000818:Animals; D002417:Cattle; D007124:Immunoenzyme Techniques; D017403:In Situ Hybridization; D009412:Nerve Fibers / Q000378:metabolism; D009474:Neurons / Q000378:metabolism*; D009481:Neurophysins / Q000378:metabolism; D010121:Oxytocin / Q000235:genetics* / Q000378:metabolism*; D010870:Pineal Gland / Q000294:innervation*; D012333:RNA, Messenger / Q000378:metabolism; D011863:Radioimmunoassay
chemical_list: D009481:Neurophysins; D012333:RNA, Messenger; D010121:Oxytocin