Microfabricated polymer chip for capillary gel electrophoresis.
A polymer (PDMS: poly(dimethylsiloxane)) microchip for capillary gel electrophoresis that can separate different sizes of DNA molecules in a small experimental scale is presented. This microchip can be easily produced by a simple PDMS molding method against a microfabricated master without the use of elaborate bonding processes. This PDMS microchip could be used as a single use device unlike conventional microchips made of glass, quartz or silicon. The capillary channel on the chip was partially filled with agarose gel that can enhance separation resolution of different sizes of DNA molecules and can shorten the channel length required for the separation of the sample compared to capillary electrophoresis in free-flow or polymer solution format. We discuss the optimal conditions for the gel preparation that could be used in the microchannel. DNA molecules were successfully driven by an electric field and separated to form bands in the range of 100 bp to 1 kbp in a 2.0% agarose-filled microchannel with 8 mm of effective separation length.
Hong
J W
JW
Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Tokyo 113-8656, Japan.
Hosokawa
K
K
Fujii
T
T
Seki
M
M
Endo
I
I
D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D004247:DNA / Q000302:isolation & purification*; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000592:standards; D008832:Microchemistry; D008856:Microscopy, Fluorescence; D018715:Microscopy, Video; D008904:Miniaturization; D008970:Molecular Weight; D015203:Reproducibility of Results; D012685:Sepharose; D012828:Silicones / Q000737:chemistry*
D020099:Coated Materials, Biocompatible; D004129:Dimethylpolysiloxanes; D012828:Silicones; C013830:baysilon; D004247:DNA; D012685:Sepharose