title: Programmed translational frameshifting is likely required for expressions of genes encoding putative nuclear protein kinases of the ciliate Euplotes octocarinatus.
abstract: Three macronuclear genes encoding putative nuclear protein kinases of the ciliate Euplotes octocarinatus syngen 1 were isolated and sequenced. All three deduced gene products share significant properties with a group of recently identified nuclear serine/threonine protein kinases named Ndr. The three predicted proteins contain the twelve conserved catalytic subdomains of protein kinases and 22 near universally-conserved amino acids residues that are characteristic of serine/threonine protein kinases. In addition, there is an approximately 30 amino acid-peptide insertion between subdomains VII and VIII that contains a potential nuclear localization signal. Sequence analysis suggests that expression of the Eondr2 gene requires a + 1 programmed translational frameshift for its translation. Comparison of the deduced EoNdr2 with other known Ndr protein kinases implies that a + 1 ribosomal frameshift occurs at the motif AAATAA.
authors.affiliation: Institut für Allgemeine Zoologie und Genetik, Universität Münster, Germany. ming.tan@uc.edu
authors.forename: M
authors.initials: M
authors.lastname: Tan
authors.forename: A
authors.initials: A
authors.lastname: Liang
authors.forename: C
authors.initials: C
authors.lastname: Brünen-Nieweler
authors.forename: K
authors.initials: K
authors.lastname: Heckmann
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002467:Cell Nucleus / Q000201:enzymology*; D002875:Chromosomes / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D016054:DNA, Protozoan / Q000032:analysis; D016812:Euplotes / Q000201:enzymology* / Q000235:genetics; D018965:Frameshifting, Ribosomal; D018628:Gene Dosage; D008969:Molecular Sequence Data; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D018076:DNA, Complementary; D016054:DNA, Protozoan; D017346:Protein Serine-Threonine Kinases