Suppressing effect of substance P receptor antagonist on sound evoked potentials recorded from the guinea pig cochlea.
OBJECTIVE
To investigate the regulation of SP in the cochlea function.


METHODS
Ten adult guinea pigs were used as experimental animals. The perilymph space of the guinea pigs cochlea was perfused with artificial perilymph solution containing 1 microgram/microliter SP receptor antagonist (D-Arg1, D-Pro2, D-Trp7,9, Leu11)-SP (1-11) at a rate of 2.5 microliters/min for 10 min while monitoring cochlear potentials evoked by 4 kHz tone burst.


RESULTS
The perfusion of SP antagonist resulted in a suppression of the compound action potential of the auditory nerve (CAP, N1-P1), a prolongation of the N1 latency at threshold and suprathreshold levels, an elevation of the CAP threshold.


CONCLUSIONS
These results suggest that SP might play a role as a transmitter or modulator in the cochlear function.
Liang
X
X
Department of Otolaryngology, Peking Union Medical College Hospital, Chinese Academy of Medical Scienses, Peking Union Medical College, Beijing 100730, China.
Wang
Z
Z
Zhang
L
L
Ni
D
D
D000200:Action Potentials; D000818:Animals; D003051:Cochlea / Q000502:physiology*; D003056:Cochlear Nerve / Q000502:physiology; D005072:Evoked Potentials, Auditory; D005260:Female; D006168:Guinea Pigs; D008297:Male; D064729:Neurokinin-1 Receptor Antagonists; D011994:Recombinant Proteins; D013373:Substance P / Q000031:analogs & derivatives / Q000494:pharmacology*
D064729:Neurokinin-1 Receptor Antagonists; D011994:Recombinant Proteins; D013373:Substance P; C050702:substance P, Arg(1)-Pro(2)-Trp(7,9)-Leu(11)-