CD8 CTL responses in vaccines: emerging patterns of HLA restriction and epitope recognition.
We evaluated MHC-class I-restricted CTL responses induced by HIV-1 clade B-based vaccines in nine HIV-1 seronegative vaccine recipients with regard to their patterns of HLA restriction and epitope recognition. We found that seven of nine volunteers developed detectable CTL reactivities against novel epitopes within the HIV-1 Env and Gag proteins. Although four of nine subjects were HLA-A*0201, none of the cellular responses was restricted in the context of this allele. The type of responses observed in this sampling of vaccines appeared similar to those reported during primary infection and among long term non-progressors, with three out of nine subjects recognizing HLA-B27 or HLA-B17(57)-restricted epitopes. Although the majority of CTL responses were directed against novel epitopes, these effectors were still able to mediate cross-clade reactivities.
Ferrari
G
G
Department of Surgery, Duke University Medical Center, P.O. Box 2926, Durham, NC 27710, USA. gflmp@acpub.duke.edu
Neal
W
W
Jones
A
A
Olender
N
N
Ottinger
J
J
Ha
R
R
McElrath
M J
MJ
Goepfert
P
P
Weinhold
K J
KJ
D016915:AIDS Vaccines / Q000494:pharmacology*; D003429:Cross Reactions; D018604:Epitope Mapping; D000939:Epitopes; D015686:Gene Products, env / Q000276:immunology; D015683:Gene Products, gag / Q000276:immunology; D015488:HIV Antigens; D018023:HIV Seronegativity / Q000235:genetics / Q000276:immunology; D015497:HIV-1 / Q000276:immunology; D006680:HLA Antigens / Q000235:genetics; D006801:Humans; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D016915:AIDS Vaccines; D000939:Epitopes; D015686:Gene Products, env; D015683:Gene Products, gag; D015488:HIV Antigens; D006680:HLA Antigens