Antioxidant effects of D002 on the in vitro susceptibility of whole plasma in healthy volunteers.
BACKGROUND
It has been recently shown that oral administration of D002, a mixture of higher aliphatic primary alcohols isolated from beeswax, inhibits rat microsomal lipid peroxidation. This justified the present attempt to investigate whether D002 also exerts antioxidant effects in humans.


METHODS
The effects of D002 on lipid peroxidation were studied in a double-blind, randomized, placebo-controlled trial conducted in 50 healthy volunteers. Unfractionated plasma samples at baseline and at 12 weeks were subjected to in vitro copper-induced lipid peroxidation and conjugated diene generation was monitored by changes of optical density.


RESULTS
The oral treatment with D002 (50 mg/day) not only significantly prolonged (p <0.001) lag time before the onset of conjugated diene formation compared with that of baseline but also increased (p <0.05) lag phase when compared with placebo group. In fact, in the D002 group the lag-phase of oxidation was prolonged 1.5-fold. D002 oral treatment decreased TBARS and increased plasma total antioxidant status (TAS) (p <0.01).


CONCLUSIONS
Because prooxidant states have been linked to normal senescence and some age-related diseases, the present data suggest that D002 may find a use in preventing age-related diseases as a dietary natural antioxidant supplement.
Menéndez
R
R
Laboratorio de Bioquímica, Centro de Productos Naturales, Centro Nacional para Investigación Científica, Havana, Cuba. dalmer@ip.etecsa.cu
Más
R
R
Amor
A M
AM
Pérez
Y
Y
González
R M
RM
Fernández
J
J
Molina
V
V
Jiménez
S
S
D000284:Administration, Oral; D000328:Adult; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology*; D001798:Blood Proteins / Q000187:drug effects*; D003300:Copper / Q000494:pharmacology; D004311:Double-Blind Method; D005233:Fatty Alcohols / Q000008:administration & dosage / Q000494:pharmacology*; D005260:Female; D005979:Glutathione Peroxidase / Q000097:blood; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D008875:Middle Aged; D010084:Oxidation-Reduction; D012016:Reference Values; D013482:Superoxide Dismutase / Q000097:blood; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis
D000975:Antioxidants; D001798:Blood Proteins; D005233:Fatty Alcohols; D017392:Thiobarbituric Acid Reactive Substances; D003300:Copper; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase