title: Differentially expressed genes in association with in vitro invasiveness of human epithelioid sarcoma.
abstract: OBJECTIVE
  Differential display reverse transcription polymerase chain reaction (RT-PCR) was performed to identify genes associated with the invasive potential of human epithelioid sarcoma.
  
  
  METHODS
  Two different clonal subpopulations, GRU-1A and GRU-1B, derived from the same human epithelioid sarcoma cell line GRU-1 and known to differ greatly in their invasive potential were compared by means of mRNA fingerprinting.
  
  
  RESULTS
  Using a set of 10 arbitrary upstream primers and nine anchored oligo-dT primers, 22 candidate gene fragments were identified; differential expression was confirmed in four of these fragments by northern blot analysis. At the mRNA level, apoferritin light chain was predominantly expressed by the highly invasive cell line GRU-1A. In contrast, the mitochondrial gene M1, encoding cytochrome c oxidase I, and the TI-227H gene were expressed more strongly by the low invasive cell line GRU-1B. Furthermore, a novel gene fragment was identified and cloned that was preferentially expressed in the low invasive cell line GRU-1B, and therefore might have an inhibitory role in invasion. Consequently, this gene fragment was designated as expressed in low invasive sarcoma cells (ELISC-1).
  
  
  CONCLUSIONS
  A novel gene fragment (ELISC-1) and three known genes were identified as potential regulators of tumour invasiveness. Cloning of the entire sequence of ELISC-1 and subsequent investigations are required to establish its biological role.
authors.affiliation: Institute of Pathology, Heinrich-Heine-University, Moorenstr. 5, D-40225 Duesseldorf, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Weber
authors.forename: R
authors.initials: R
authors.lastname: Engers
authors.forename: S
authors.initials: S
authors.lastname: Nockemann
authors.forename: L L
authors.initials: LL
authors.lastname: Gohr
authors.forename: A
authors.initials: A
authors.lastname: Zur Hausen
authors.forename: H E
authors.initials: HE
authors.lastname: Gabbert
mesh_terms: D000955:Antigens, T-Independent / Q000378:metabolism; D001052:Apoferritins / Q000378:metabolism; D015152:Blotting, Northern; D016172:DNA Fingerprinting; D003576:Electron Transport Complex IV / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D009361:Neoplasm Invasiveness / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012509:Sarcoma / Q000235:genetics*; D014407:Tumor Cells, Cultured
chemical_list: D000955:Antigens, T-Independent; D012333:RNA, Messenger; D001052:Apoferritins; D003576:Electron Transport Complex IV