The nature of the bond between peptide and carrier molecule determines the immunogenicity of the construct.
The influence of the nature of the bond between a peptide and a (lipidic) carrier molecule on the immunogenicity of that construct was investigated. As types of bonds a thioester-, a disulfide-, an amide- and a thioether bond were investigated. As carrier molecules a peptide, an N-palmitoylated peptide or a C(16)-hydrocarbon chain were used. The biostability of the bond between peptide and carrier molecule is thioether > amide > disulfide >> thioester. However, the immunogenic potency of the constructs used was found to be thioester > disulfide > amide > thioether. In conclusion, a construct with a bond between peptide and (lipidic) carrier molecule that is more susceptible to biological degradation is more immunogenic when used in a peptide-based vaccine than a bond that is less susceptible to biological degradation.
Beekman
N J
NJ
Institute for Animal Science and Health (ID-Lelystad), Department of Molecular Recognition, Lelystad, The Netherlands.
Schaaper
W M
WM
Langeveld
J P
JP
Boshuizen
R S
RS
Meloen
R H
RH
D000577:Amides / Q000737:chemistry; D000818:Animals; D000906:Antibodies / Q000737:chemistry / Q000276:immunology*; D004220:Disulfides / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry*; D005260:Female; D029544:Foot-and-Mouth Disease Virus / Q000737:chemistry / Q000276:immunology; D007987:Gonadotropin-Releasing Hormone / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D006168:Guinea Pigs; D006838:Hydrocarbons / Q000737:chemistry / Q000276:immunology*; D008297:Male; D008956:Models, Chemical; D019308:Palmitic Acid / Q000737:chemistry; D017993:Parvovirus, Canine / Q000737:chemistry / Q000276:immunology*; D010455:Peptides / Q000737:chemistry / Q000276:immunology; D013440:Sulfides / Q000737:chemistry; D013552:Swine; D022223:Vaccines, Subunit / Q000737:chemistry* / Q000276:immunology*
D000577:Amides; D000906:Antibodies; D004220:Disulfides; D004337:Drug Carriers; D006838:Hydrocarbons; D010455:Peptides; D013440:Sulfides; D022223:Vaccines, Subunit; D019308:Palmitic Acid; D007987:Gonadotropin-Releasing Hormone