Effects of slow clinorotation on lipid contents and proton permeability of thylakoid membranes of pea chloroplasts.
Photochemical characteristics and lipid composition of thylakoid membranes from 12 day-old pea leaves that were exposed to slow clino-rotation were examined and compared with a vertical control. Proton permeability of thylakoid membranes was estimated from light-induced proton uptake (delta H+) and post-illumination proton efflux in chloroplast suspensions. The delta pH magnitude was calculated from the level of light-induced quenching of 9-aminoacridine fluorescence. Proton permeability of thylakoid membranes increased during exposure to clino-rotation. When subsequently transferred to darkness, proton efflux increased almost 2-fold in clinorotated leaves. The results were compared with data on pigment and polar lipid composition of photosynthetic membranes in clino-rotated and control plants. It was concluded that both the increase of proton permeability and the decrease of polar lipid content in chloroplasts were induced by clino-rotation.
Mikhaylenko
N F
NF
M.G. Kholodny Institute of Botany, National Academy of Sciences of the Ukraine, Kyiv, Ukraine.
Sytnik
S K
SK
Zolotareva
E K
EK
D002338:Carotenoids / Q000378:metabolism; D002734:Chlorophyll / Q000378:metabolism; D000077194:Chlorophyll A; D002736:Chloroplasts / Q000378:metabolism / Q000528:radiation effects; D006017:Glycolipids / Q000378:metabolism; D006112:Gravitation; D006863:Hydrogen-Ion Concentration; D008027:Light; D008051:Lipid Bilayers; D050356:Lipid Metabolism; D018532:Pisum sativum / Q000254:growth & development / Q000378:metabolism* / Q000528:radiation effects; D010743:Phospholipids / Q000378:metabolism; D017494:Proton Pumps / Q000378:metabolism* / Q000528:radiation effects; D012399:Rotation; D020524:Thylakoids / Q000378:metabolism* / Q000528:radiation effects; D018474:Weightlessness Simulation
D006017:Glycolipids; D008051:Lipid Bilayers; D010743:Phospholipids; D017494:Proton Pumps; D002734:Chlorophyll; D002338:Carotenoids; C037184:chlorophyll b; D000077194:Chlorophyll A