title: Molecular cloning and embryonic expression of the Xenopus Arnt gene.
abstract: In this paper, we report the cloning of a Xenopus bHLH/PAS factor homologous to the mammalian aryl hydrocarbon receptor nuclear translocator (Arnt) or Drosophila Tango gene. Sequence data analysis indicates that protein domains organization in xArnt is strongly conserved and that xArnt is highly related to the mammalian Arnt1 isoform. As revealed by reverse transcriptase polymerase chain reaction and whole-mount in situ hybridization, xArnt gene is expressed during early and late development. At early stages, xArnt transcripts are restricted to the ectoderm and extends to the marginal zone at gastrula stage. In tail bud embryo, xArnt is strongly expressed in branchial arches, optical and optical vesicles, and pronephros and pronephritic duct.
authors.affiliation: Université Pierre et Marie Curie, Groupe Genes et Developpement, UMR7622-CNRS Biologie Moléculaire et Cellulaire du Développement, 9 quai St Bernard, 75252 Paris Cedex 05, France.
authors.forename: K
authors.initials: K
authors.lastname: Bollérot
authors.forename: N
authors.initials: N
authors.lastname: Angelier
authors.forename: P
authors.initials: P
authors.lastname: Coumailleau
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D051784:Aryl Hydrocarbon Receptor Nuclear Translocator; D051792:Basic Helix-Loop-Helix Proteins; D002352:Carrier Proteins / Q000235:genetics; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins; D004330:Drosophila / Q000235:genetics; D029721:Drosophila Proteins; D018507:Gene Expression Regulation, Developmental; D017344:Genes, Insect; D017403:In Situ Hybridization; D019476:Insect Proteins / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D018336:Receptors, Aryl Hydrocarbon; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000235:genetics*; D029867:Xenopus Proteins; D014982:Xenopus laevis / Q000196:embryology* / Q000235:genetics*
chemical_list: D051784:Aryl Hydrocarbon Receptor Nuclear Translocator; D051792:Basic Helix-Loop-Helix Proteins; D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D019476:Insect Proteins; D018336:Receptors, Aryl Hydrocarbon; D014157:Transcription Factors; D029867:Xenopus Proteins; C490992:ARNT2 protein, Xenopus; C497397:Arnt protein, mouse; C099817:Arnt2 protein, mouse; C109766:tgo protein, Drosophila