Effect of mobile phase acidic additives on enantioselectivity for phenylalanine analogs.
The use of acidic mobile phase additives allows the chiral separation of underivatized phenylalanine analogs on a common amylosic column. In addition to decreasing retention and band-broadening arising from non-ideal interactions, acidic additives may also increase selectivity. This appears to be due to the minimization of non-selective binding in the recognition site. Effects of the additives are related to additive pKa and size. Ethanesulfonic acid was typically the most effective additive with trifluoroacetic acid being one of the least effective.
Ye
Y K
YK
DuPont Pharmaceutical Company, Chemical Process Research and Development, Deepwater, NJ 08023, USA. yun.k.ye@dupontpharma.com
Stringham
R W
RW
D000143:Acids / Q000737:chemistry*; D010649:Phenylalanine / Q000031:analogs & derivatives*; D012680:Sensitivity and Specificity; D013237:Stereoisomerism
D000143:Acids; D010649:Phenylalanine