Multiple transcripts generated by the DCAMKL gene are expressed in the rat hippocampus.
We have recently cloned a novel Doublecortin CaMK-like kinase (rDCAMKL) cDNA, and a related cDNA called CaMK-related peptide (CARP) from the rat hippocampus. These genes are structurally highly similar to the human DCAMKL-1 gene and doublecortin, a gene associated with X-linked lissencephaly and subcortical band heterotopia. Here we report on the genomic organization of the murine DCAMKL gene and its products. Our results show that DCAMKL and CARP are alternative splice products of the same gene. The DCAMKL gene also generates three alternatively-spliced rDCAMKL transcripts of which we have cloned the corresponding cDNAs and which potentially generate different DCAMKL proteins. In situ hybridization experiments show that the different rDCAMKL transcripts are all expressed in the adult rat hippocampus. We conclude that alternative splicing of the DCAMKL gene may generate different but similar proteins in the adult rat hippocampus thereby regulating different but overlapping aspects of DCAMKL controlled neuronal plasticity.
Vreugdenhil
E
E
Division of Medical Pharmacology, Center for Drug Research (LACDR), Leiden University, P.O. Box 9503, 2300 RA, Leiden, The Netherlands
Engels
B
B
Middelburg
R
R
van Koningsbruggen
S
S
Knol
J
J
Veldhuisen
B
B
de Kloet
E R
ER
D000367:Age Factors; D017398:Alternative Splicing / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D054729:Calcium-Calmodulin-Dependent Protein Kinase Type 1; D054734:Calcium-Calmodulin-Dependent Protein Kinase Type 4; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000235:genetics*; D002352:Carrier Proteins; D003599:Cytoskeleton / Q000201:enzymology; D018076:DNA, Complementary / Q000302:isolation & purification; D000089622:Doublecortin Domain Proteins; D000089662:Doublecortin Protein; D005091:Exons; D005260:Female; D015870:Gene Expression / Q000502:physiology; D006624:Hippocampus / Q000502:physiology*; D006801:Humans; D007438:Introns; D051379:Mice; D008869:Microtubule-Associated Proteins; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000235:genetics; D010750:Phosphoproteins / Q000235:genetics; D051381:Rats; D014158:Transcription, Genetic / Q000502:physiology*
D002352:Carrier Proteins; D018076:DNA, Complementary; C000714847:Dcx protein, mouse; C000714848:Dcx protein, rat; D000089622:Doublecortin Domain Proteins; D000089662:Doublecortin Protein; D008869:Microtubule-Associated Proteins; D009479:Neuropeptides; D010750:Phosphoproteins; C461219:RNF34 protein, human; C517596:CAMK1 protein, human; C517683:CAMK4 protein, human; D054729:Calcium-Calmodulin-Dependent Protein Kinase Type 1; D054734:Calcium-Calmodulin-Dependent Protein Kinase Type 4; D017871:Calcium-Calmodulin-Dependent Protein Kinases; C517598:Camk1 protein, mouse; C517599:Camk1 protein, rat; C517685:Camk4 protein, mouse; C517686:Camk4 protein, rat; C517681:Pnck protein, mouse; C517682:Pnck protein, rat