[Sequencing-based typing of HLA-DPB1 and DQB1 exon 2 in patients with type 1 diabetes mellitus].
OBJECTIVE
To investigate the correlation between type 1 diabetes mellitus susceptibility and HLA-DPB1 and DQB1 genes in Shandong Han population.


METHODS
HLA-DPB1 and DQB1 genes analysis were performed by sequencing-based typing in 52 type 1 diabetes mellitus patients and 38 normal controls.


RESULTS
The frequency of DPB1*2201 in diabetics was significantly higher than that in controls(26.92% vs 5.26%, P<0.01), but the frequency of DPB1*0402 in diabetics was significantly lower than that in controls(11.54% vs 34.21%, P<0.01). The frequencies of DQB1*0201, *0303 and *0604 in diabetics were higher than those in controls (24.04% vs 10.53%, P< 0.01; 22.12% vs 6.58%, P<0.05; 19.23% vs 7.89%, P<0.05), while the frequency of DQB1*0301 in diabetics was significantly lower than that in controls (4.81% vs 25.00%, P<0.01).


CONCLUSIONS
DPB1*2201, DQB1*0201, *0303 and *0604 may be susceptible alleles, and DPB1*0402 and DQB1*0301 may be protective alleles in patients with type 1 diabetes mellitus.
Zhang
S
S
General Hospital of Jinan Command, PLA, Jinan, Shandong, 250031 P.R. China.
Xing
W
W
Ke
B
B
Yan
H
H
Jiang
Z
Z
D000293:Adolescent; D000328:Adult; D001483:Base Sequence; D003922:Diabetes Mellitus, Type 1 / Q000235:genetics*; D005091:Exons; D005260:Female; D005787:Gene Frequency; D006682:HLA-DP Antigens / Q000235:genetics*; D059868:HLA-DP beta-Chains; D006683:HLA-DQ Antigens / Q000235:genetics*; D059866:HLA-DQ beta-Chains; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data
D006682:HLA-DP Antigens; D059868:HLA-DP beta-Chains; C071170:HLA-DPB1 antigen; D006683:HLA-DQ Antigens; D059866:HLA-DQ beta-Chains; C069051:HLA-DQB1 antigen