Preservation of neurotrophin expression in microglia that migrate into the gerbil's brain across the blood-brain barrier.
Microglia isolated from a mixed glial culture drawn from neonatal Mongolian gerbils were demonstrated to produce high amounts of brain-derived neurotrophic factor (BDNF) and glial cell line-derived neurotrophic factor (GDNF). The gerbil microglia retained the capability to migrate into the brain parenchyma after intra-arterial injection. We found that exogenously migrated microglia retained their BDNF and GDNF productive ability and expressed large amounts of BDNF and GDNF in damaged brain areas which suggests microglia's role as a protectant of damaged neurons. Since peripherally injected microglia exhibit specific affinity for areas of neural damage within the brain, we suggest that microglia are possible tools for cell therapy of brain damage.
Suzuki
H
H
Institute for Comprehensive Medical Science, Fujita Health University, Toyoake, Aichi 470-1192, Japan.
Imai
F
F
Kanno
T
T
Sawada
M
M
D000818:Animals; D000831:Animals, Newborn; D001812:Blood-Brain Barrier / Q000502:physiology*; D001921:Brain / Q000166:cytology / Q000378:metabolism / Q000601:surgery*; D001930:Brain Injuries / Q000378:metabolism / Q000503:physiopathology / Q000628:therapy*; D016380:Brain Tissue Transplantation / Q000379:methods*; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology*; D002478:Cells, Cultured / Q000187:drug effects / Q000378:metabolism / Q000637:transplantation; D005456:Fluorescent Dyes; D005849:Gerbillinae; D051100:Glial Cell Line-Derived Neurotrophic Factor; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism; D007150:Immunohistochemistry; D007371:Interferon-gamma / Q000494:pharmacology; D008297:Male; D017628:Microglia / Q000187:drug effects / Q000378:metabolism / Q000637:transplantation*; D009414:Nerve Growth Factors / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009930:Organic Chemicals
D019208:Brain-Derived Neurotrophic Factor; D005456:Fluorescent Dyes; D051100:Glial Cell Line-Derived Neurotrophic Factor; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D009930:Organic Chemicals; C070080:PKH 26; D007371:Interferon-gamma