A transformation system for an ectomycorrhizal basidiomycete, Lyophyllum shimeji.
A transformation vector, pLS-hph, was constructed with the promoter and terminator of the glyceraidehyde-3-phosphate dehydrogenase (GPD) gene derived from an ectomycorrhizal basidiomycete, Lyophyllum shimeji, and with the hygromycin B (HmB) phosphotransferase (hph) gene from Escherichia coli. This vector was introduced into protoplasts of L. shimeji and 3.4 transformants per microg plasmid DNA were obtained. In most of the transformants, multiple copies of the vector were integrated into the genomic DNA. The results indicate that pLS-hph is a useful vector for L. shimeji.
Saito
T
T
Department of Biological and Chemical Engineering, Faculty of Engineering, Gunma University, Kiryu, Japan.
Tanaka
N
N
Shinozawa
T
T
D001487:Basidiomycota / Q000235:genetics* / Q000378:metabolism*; D015139:Blotting, Southern; D003470:Culture Media; D004271:DNA, Fungal / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005800:Genes, Fungal / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics*; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D013559:Symbiosis / Q000502:physiology; D014170:Transformation, Genetic / Q000235:genetics*; D014197:Trees / Q000382:microbiology
D003470:Culture Media; D004271:DNA, Fungal; D017853:Phosphotransferases (Alcohol Group Acceptor); C063242:hygromycin-B kinase