DNA-immunization with a V2 deleted HIV-1 envelope elicits protective antibodies in macaques.
Rhesus macaques immunized with the HIV-1 SF162DeltaV2 gp140 envelope using the DNA-prime plus protein-boost vaccination methodology, developed HIV envelope-specific T-cell lymphoproliferative responses and potent neutralizing antibodies. To evaluate the protective potential of these antibodies during acute infection, the animals were depleted of their CD8+ T lymphocytes using specific monoclonal antibodies and subsequently challenged intravenously with the pathogenic SHIV(SF162P4) isolate. As compared to non-vaccinated animals (one of which died from AIDS 16 weeks post-exposure) the vaccinated macaques had lower levels of peak viremia, rapidly cleared virus from the periphery and developed delayed seroconversion to SIV core antigens.
Cherpelis
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Aaron Diamond AIDS Research Center, The Rockefeller University, New York, NY 10021-6399, USA.
Jin
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Gettie
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A
Ho
D D
DD
Barnett
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SW
Shrivastava
I
I
Stamatatos
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L
D016915:AIDS Vaccines / Q000494:pharmacology*; D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D015686:Gene Products, env / Q000235:genetics / Q000276:immunology*; D015483:HIV Antibodies / Q000096:biosynthesis*; D015497:HIV-1 / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D008213:Lymphocyte Activation; D008212:Lymphocyte Depletion; D008253:Macaca mulatta / Q000276:immunology*; D009500:Neutralization Tests; D018449:SAIDS Vaccines / Q000494:pharmacology; D017384:Sequence Deletion; D015302:Simian Immunodeficiency Virus / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D019444:Vaccines, DNA / Q000494:pharmacology*; D054299:env Gene Products, Human Immunodeficiency Virus
D016915:AIDS Vaccines; D015686:Gene Products, env; D015483:HIV Antibodies; D018449:SAIDS Vaccines; D019444:Vaccines, DNA; D054299:env Gene Products, Human Immunodeficiency Virus; C097729:gp140 envelope protein, Human immunodeficiency virus 1