A rapid, automated approach to optimisation of multiple reaction monitoring conditions for quantitative bioanalytical mass spectrometry.
An improvement to the procedure for the rapid optimisation of mass spectrometry (PROMS), for the development of multiple reaction methods (MRM) for quantitative bioanalytical liquid chromatography/tandem mass spectrometry (LC/MS/MS), is presented. PROMS is an automated protocol that uses flow-injection analysis (FIA) and AppleScripts to create methods and acquire the data for optimisation. The protocol determines the optimum orifice potential, the MRM conditions for each compound, and finally creates the MRM methods needed for sample analysis. The sensitivities of the MRM methods created by PROMS approach those created manually. MRM method development using PROMS currently takes less than three minutes per compound compared to at least fifteen minutes manually. To further enhance throughput, approaches to MRM optimisation using one injection per compound, two injections per pool of five compounds and one injection per pool of five compounds have been investigated. No significant difference in the optimised instrumental parameters for MRM methods were found between the original PROMS approach and these new methods, which are up to ten times faster. The time taken for an AppleScript to determine the optimum conditions and build the MRM methods is the same with all approaches.
Higton
D M
DM
CEDD Drug Metabolism & Pharmacokinetics, GlaxoSmithKline Research and Development, Park Road, Ware, Hertfordshire SG12 0DP, UK. dmh0372@glaxowellcome.co.uk
D000420:Albuterol / Q000031:analogs & derivatives* / Q000032:analysis; D001331:Automation / Q000295:instrumentation* / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004353:Drug Evaluation, Preclinical; D013058:Mass Spectrometry / Q000379:methods; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D000068299:Salmeterol Xinafoate; D012680:Sensitivity and Specificity; D012984:Software; D013997:Time Factors
D004364:Pharmaceutical Preparations; D000068299:Salmeterol Xinafoate; D000420:Albuterol