title: In vitro study of the effect of titanium on porphyromonas gingivalis in the presence of metronidazole and spiramycin.
abstract: Septic peri-implantitis is the main clinical complication encountered following the insertion of titanium implants. It may be resistant to conventional antibiotic treatments. Reports in the literature about antibiotic behavior in the presence of titanium remain controversial. They vary from a bacteriostat to a decreased effect of antibiotic. This study examined, in vitro, the viability of Porphyromonas gingivalis, frequently associated with periodontal diseases, in the presence of titanium and antibiotics (spiramycin and metronidazole alone or in combination). Viability of P. gingivalis was determined, versus a standard curve using the Live/dead Baclight Bacteria Viability Kit on 96 well microplates. The results of 48 experiments (60 measurements each) were compiled in a database and compared to each other using the chi2p < 0.05 test. When used alone, titanium enhanced bacterial growth as the nickel-chrome control. However, when titanium was used in the presence of antibiotics, antibiotics kept their own effects. Even more, titanium was shown to potentialize the effect of metronidazole. The strengthening of effectiveness of metronidazole by titanium may be due to the oxidation potential of the metal. This chemical property could explain the conflicting data reported in the literature.
authors.affiliation: Equipe de Biologie Buccale, UPRES-EA 1256, Université de Rennes 1, France. laurence.bunetel@univ-rennes1.fr
authors.forename: L
authors.initials: L
authors.lastname: Bunetel
authors.forename: J
authors.initials: J
authors.lastname: Guérin
authors.forename: G
authors.initials: G
authors.lastname: Agnani
authors.forename: S
authors.initials: S
authors.lastname: Piel
authors.forename: H
authors.initials: H
authors.lastname: Pinsard
authors.forename: J C
authors.initials: JC
authors.lastname: Corbel
authors.forename: M
authors.initials: M
authors.lastname: Bonnaure-Mallet
mesh_terms: D000900:Anti-Bacterial Agents / Q000008:administration & dosage; D001672:Biocompatible Materials / Q000494:pharmacology*; D015921:Dental Implants / Q000009:adverse effects; D004357:Drug Synergism; D006801:Humans; D066298:In Vitro Techniques; D008422:Materials Testing; D008795:Metronidazole / Q000008:administration & dosage; D016966:Porphyromonas gingivalis / Q000187:drug effects* / Q000472:pathogenicity*; D016459:Prosthesis-Related Infections / Q000209:etiology / Q000517:prevention & control; D015572:Spiramycin / Q000008:administration & dosage; D014025:Titanium / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D015921:Dental Implants; D008795:Metronidazole; D015572:Spiramycin; D014025:Titanium