title: Immunohistochemical determination of estrogen and progesterone receptors and DNA flow cytometry in endometrial cancer.
abstract: Estrogen and progesterone receptor contents (ER, PR) were assessed by an immunohistochemical method and DNA ploidy and S-phase by flow cytometry in frozen endometrial cancer tissue sections from 39 cases. Comparison of the immunohistochemical and cytosol assays showed 81% and 84% concordance in ER and PR contents, respectively. An aneuploid DNA pattern was identified in 30% and a high S-phase fraction was found in 33% of 36 specimens studied. Negative ER status was associated with aneuploid and high S-phase fraction. A similar association was found between PR status and high S-phase fraction. Combined analysis of immunohistochemical receptor status and DNA flow cytometry in the same sample makes it possible to identify two strong predictive factors in endometrial adenocarcinoma.
authors.affiliation: Departments of Clinical and Biomedical Sciences, University of Tampere, and Department of Obstetrics and Gynecology, University Hospital, Tampere, Finland.
authors.forename: P.K.
authors.initials: PK
authors.lastname: Heinonen
authors.forename: J.
authors.initials: J
authors.lastname: Isola
authors.forename: T.
authors.initials: T
authors.lastname: Kuoppala