Inhibitory effect of epigallocatechin gallate on adhesion of murine melanoma cells to laminin.
We examined the effects of five kinds of green tea catechin on the adhesion of mouse melanoma B16 cells to laminin. (-)-Epigallocatechin gallate (EGCG) and (-)-epicatechin gallate in the culture medium were found to inhibit the cell adhesion. The adhesion to laminin pre-treated with EGCG was also impaired. Affinity chromatography revealed the binding affinity between laminin and EGCG. These data suggest that the inhibitory effect of EGCG on adhesion of melanoma cells to laminin is included in the mechanism(s) of previously reported metastasis inhibition elicited by EGCG and green tea infusion.
Suzuki
Y
Y
School of Food and Nutritional Sciences, University of Shizuoka, Yada, Shizuoka 422-8526, Japan.
Isemura
M
M
D000818:Animals; D000970:Antineoplastic Agents / Q000378:metabolism / Q000494:pharmacology*; D002392:Catechin / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects*; D002846:Chromatography, Affinity; D003094:Collagen / Q000378:metabolism; D007797:Laminin / Q000378:metabolism*; D008546:Melanoma, Experimental / Q000473:pathology*; D051379:Mice; D009362:Neoplasm Metastasis; D019096:Vitronectin / Q000378:metabolism
D000970:Antineoplastic Agents; D007797:Laminin; D019096:Vitronectin; D002392:Catechin; D003094:Collagen; C045651:epigallocatechin gallate