title: Histones H3/H4 form a tight complex with the inner nuclear membrane protein LBR and heterochromatin protein 1.
abstract: We have recently shown that heterochromatin protein 1 (HP1) interacts with the nuclear envelope in an acetylation-dependent manner. Using purified components and in vitro assays, we now demonstrate that HP1 forms a quaternary complex with the inner nuclear membrane protein LBR and a sub-set of core histones. This complex involves histone H3/H4 oligomers, which mediate binding of LBR to HP1 and cross-link these two proteins that do not interact directly with each other. Consistent with previous observations, HP1 and LBR binding to core histones is strongly inhibited when H3/H4 are modified by recombinant CREB-binding protein, revealing a new mechanism for anchoring domains of under-acetylated chromatin to the inner nuclear membrane.
authors.affiliation: Department of Basic Sciences, The University of Crete, School of Medicine, 71 110 Heraklion, Crete, Greece.
authors.forename: H
authors.initials: H
authors.lastname: Polioudaki
authors.forename: N
authors.initials: N
authors.lastname: Kourmouli
authors.forename: V
authors.initials: V
authors.lastname: Drosou
authors.forename: A
authors.initials: A
authors.lastname: Bakou
authors.forename: P A
authors.initials: PA
authors.lastname: Theodoropoulos
authors.forename: P B
authors.initials: PB
authors.lastname: Singh
authors.forename: T
authors.initials: T
authors.lastname: Giannakouros
authors.forename: S D
authors.initials: SD
authors.lastname: Georgatos
mesh_terms: D000107:Acetylation; D000818:Animals; D001665:Binding Sites; D015153:Blotting, Western; D002467:Cell Nucleus / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D005399:Fishes; D005982:Glutathione Transferase / Q000378:metabolism; D006570:Heterochromatin / Q000378:metabolism; D006657:Histones / Q000378:metabolism*; D007425:Intracellular Membranes / Q000378:metabolism; D051379:Mice; D008954:Models, Biological; D010957:Plasmids / Q000378:metabolism; D011233:Precipitin Tests; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D014422:Turkeys
chemical_list: D006570:Heterochromatin; D006657:Histones; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D005982:Glutathione Transferase