title: Extrusion of actin-positive strands from Hep-2 and Int 407 cells caused by outer membrane preparations of enteropathogenic Escherichia coil and specific attachment of wild type bacteria to the strands.
abstract: Enteropathogenic Escherichia coli (EPEC) causes persistent infantile diarrhoea. This nontoxigenic E. coli exhibits a complicated pathogenic mechanism in which its outer membrane proteins and type III secretory proteins damage intestinal epithelium and cause diarrhoea. In accordance with this, our previous study using HEp-2 cells demonstrated cytopathic effects caused by cell-free outer membrane preparations of EPEC. In this study, we report the extrusion of actin-positive strands from HEp-2 and Int 407 cells when treated with outer membrane preparations. An interesting observation of this work, perhaps relevant to the characteristic localized three-dimensional colony formation of EPEC, is the attachment of a wild type EPEC strain to these actin-positive strands.
authors.affiliation: Centre for Biotechnology, Anna University, Chennai, India.
authors.forename: S S
authors.initials: SS
authors.lastname: Kumar
authors.forename: V
authors.initials: V
authors.lastname: Malladi
authors.forename: K
authors.initials: K
authors.lastname: Sankaran
authors.forename: R
authors.initials: R
authors.lastname: Haigh
authors.forename: P
authors.initials: P
authors.lastname: Williams
authors.forename: A
authors.initials: A
authors.lastname: Balakrishnan
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism* / Q000382:microbiology*; D000185:Actinin / Q000378:metabolism; D001422:Bacterial Adhesion; D001425:Bacterial Outer Membrane Proteins / Q000494:pharmacology*; D002460:Cell Line / Q000187:drug effects; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity*; D004927:Escherichia coli Infections / Q000382:microbiology; D006801:Humans
chemical_list: D001425:Bacterial Outer Membrane Proteins; D000185:Actinin