title: Light-emitting diode treatment reverses the effect of TTX on cytochrome oxidase in neurons.
abstract: Light close to and in the near-infrared range has documented benefits for promoting wound healing in human and animals. However, mechanisms of its action on cells are poorly understood. We hypothesized that light treatment with a light-emitting diode array at 670 nm (LED) is therapeutic in stimulating cellular events involving increases in cytochrome oxidase activity. LED was administered to cultured primary neurons whose voltage-dependent sodium channels were blocked by tetrodotoxin. The down-regulation of cytochrome oxidase activity by TTX was reverted to control levels by LED. LED alone also up-regulated enzyme activity. Thus, the results are consistent with our hypothesis that LED has a stimulating effect on cytochrome oxidase in neurons, even when they have been functionally silenced by TTX.
authors.affiliation: Department of Cell Biology, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA.
authors.forename: M T
authors.initials: MT
authors.lastname: Wong-Riley
authors.forename: X
authors.initials: X
authors.lastname: Bai
authors.forename: E
authors.initials: E
authors.lastname: Buchmann
authors.forename: H T
authors.initials: HT
authors.lastname: Whelan
mesh_terms: D000375:Aging / Q000502:physiology / Q000528:radiation effects; D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured / Q000187:drug effects / Q000201:enzymology / Q000528:radiation effects*; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation / Q000502:physiology / Q000528:radiation effects*; D003576:Electron Transport Complex IV / Q000378:metabolism / Q000528:radiation effects*; D007259:Infrared Rays / Q000627:therapeutic use*; D009410:Nerve Degeneration / Q000139:chemically induced / Q000201:enzymology / Q000517:prevention & control; D009474:Neurons / Q000187:drug effects / Q000201:enzymology / Q000528:radiation effects*; D010775:Photic Stimulation / Q000295:instrumentation* / Q000379:methods; D051381:Rats; D026941:Sodium Channel Blockers; D015222:Sodium Channels / Q000378:metabolism; D013779:Tetrodotoxin / Q000494:pharmacology; D014793:Visual Cortex / Q000187:drug effects / Q000201:enzymology / Q000528:radiation effects; D014945:Wound Healing / Q000502:physiology / Q000528:radiation effects*
chemical_list: D026941:Sodium Channel Blockers; D015222:Sodium Channels; D013779:Tetrodotoxin; D003576:Electron Transport Complex IV