Effects of amino acids on the amidation of polyaromatic carboxylic acids by Bacillus cereus.
The soil bacterium Bacillus cereus Tim-r01 efficiently transformed polyaromatic carboxylic acids (PACA) such as 4-biphenylcarboxylic acid (4-BPCA), 4-biphenylacetic acid, and 4-phenoxybenzoic acid into their corresponding amides. The amidation activity was expressed at 37 degrees C (pH 7-8) in the presence of grown cells in nutrients under an aerobic atmosphere. Other strains of B. cereus, IFO 3001 and IAM 1229, also gave the amide from 4-BPCA. In phosphate-buffered saline (PBS), the addition of normal amino acids was essential, while sulfur-containing amino acids such as methionine and cysteine drastically inhibited the amidation. Tracer experiments using N-15-isoleucine and N-15-alanine showed that the nitrogen atom of the amide came from an amino group of amino acids but not from ammonia or alkylamines.
Maruyama
R
R
Department of Cell Engineering, Faculty of Engineering, Toyama University, Gofuku, Japan.
Kawata
A
A
Ono
S
S
Nishizawa
M
M
Ito
S
S
Inoue
M
M
D000577:Amides / Q000378:metabolism*; D000596:Amino Acids / Q000494:pharmacology*; D001409:Bacillus cereus / Q000187:drug effects / Q000378:metabolism*; D002264:Carboxylic Acids / Q000378:metabolism*; D003470:Culture Media; D007202:Indicators and Reagents; D009682:Magnetic Resonance Spectroscopy; D009584:Nitrogen / Q000378:metabolism; D011084:Polycyclic Aromatic Hydrocarbons / Q000378:metabolism*
D000577:Amides; D000596:Amino Acids; D002264:Carboxylic Acids; D003470:Culture Media; D007202:Indicators and Reagents; D011084:Polycyclic Aromatic Hydrocarbons; D009584:Nitrogen