Sheep embryos derived from FSH/eCG treatment have a lower in vitro viability after vitrification than those derived from FSH treatment.
In the non breeding period, the effect of two superovulatory treatments (eCG/FSH in single dose or FSH alone in four decreasing doses) on the production of embryo quality following in vitro viability after vitrification procedures was investigated using forty-four adult Sarda breed ewes. In sheep treated with eCG/FSH, the mean number of corpora lutea was significantly (P < 0.05) higher (11.8+/-4.0 vs. 8.05+/-3.8), although the recovery rate was significantly (P < 0.01) lower (74.6 vs. 59.9) than with FSH alone. After vitrification (ethylene glycol and glycerol) was repeated three times, the rates of re-expansion at first and second warming were significantly (P < 0.01) higher in embryos derived from FSH alone than in those with both gonadotrophins (94.9 and 41.9 vs. 72.8 and 18.6) and after the last vitrification the hatched blastocyst rates were 22.5 and 7.6. After differential stain, blastocysts derived from FSH alone showed a mean number of cells significantly higher than blastocysts from eCG/FSH (184.2 vs. 157.7). It was concluded that superovulatory treatment with eCG/FSH may increase the ovarian responses compared with FSH alone, but these embryos showed a reduction in viability rates after repeated vitrification.
Leoni
G
G
Department of Animal Biology, University of Sassari, Italy. vetfis@ssmain.uniss.it
Bogliolo
L
L
Pintus
P
P
Ledda
S
S
Naitana
S
S
D000818:Animals; D001755:Blastocyst / Q000166:cytology / Q000187:drug effects*; D015925:Cryopreservation / Q000592:standards / Q000662:veterinary*; D003451:Cryoprotective Agents; D004624:Embryo Transfer / Q000592:standards; D004622:Embryo, Mammalian / Q000187:drug effects; D005314:Embryonic and Fetal Development; D019855:Ethylene Glycol; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology*; D005990:Glycerol; D006064:Gonadotropins, Equine / Q000494:pharmacology*; D066298:In Vitro Techniques; D011247:Pregnancy; D012756:Sheep / Q000196:embryology* / Q000502:physiology; D013480:Superovulation / Q000187:drug effects
D003451:Cryoprotective Agents; D006064:Gonadotropins, Equine; D005640:Follicle Stimulating Hormone; D019855:Ethylene Glycol; D005990:Glycerol