The inducible Hsp70 as a marker of tumor immunogenicity.
Growing evidence indicates that the stress response in general and heat shock proteins (Hsps) in particular have a profound impact on tumor immunogenicity. In this study, we show that tumor cells subjected to a nonlethal heat shock stress are unable to form tumors in syngenic mice, whereas they do so in athymic nude mice. Moreover, heat-shocked MethA immunity is tumor specific. Enhancement of T-cell-mediated immunogenicity correlates with the expression of the inducible Hsp70 but not the constitutive Hsc70. These observations have a bearing on the proposed functional role of Hsp-peptide association in antigen processing and presentation by major histocompatibility complex I molecules under normal and stressful conditions.
Clark
P R
PR
Center for Immunotherapy of Cancer and Infectious Diseases, University of Connecticut School of Medicine, Farmington 06030, USA.
Ménoret
A
A
D000230:Adenocarcinoma / Q000276:immunology / Q000473:pathology; D000818:Animals; D014408:Biomarkers, Tumor / Q000378:metabolism*; D002455:Cell Division; D002470:Cell Survival; D003110:Colonic Neoplasms / Q000276:immunology / Q000473:pathology; D004591:Electrophoresis, Polyacrylamide Gel; D018840:HSP70 Heat-Shock Proteins / Q000276:immunology*; D018869:Heat-Shock Response; D015151:Immunoblotting; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009369:Neoplasms / Q000276:immunology* / Q000378:metabolism; D012513:Sarcoma, Experimental / Q000276:immunology / Q000378:metabolism; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013997:Time Factors; D014185:Transplantation, Isogeneic / Q000276:immunology; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D014408:Biomarkers, Tumor; D018840:HSP70 Heat-Shock Proteins; D014409:Tumor Necrosis Factor-alpha