title: Skeletal muscle metabolism in overweight and post-overweight women: an isometric exercise study using (31)P magnetic resonance spectroscopy.
abstract: OBJECTIVE
  To investigate whether skeletal muscle anaerobic metabolism, oxidative metabolism or metabolic economy during controlled sub-maximal and near-maximal exercises is altered in overweight women after diet-induced weight reduction, and whether these parameters are different between normal-weight, obesity-prone and normal-weight obesity-resistant women with similar physical fitness levels.
  
  
  METHODS
  A prospective weight loss study of overweight women and their comparison with never overweight controls.
  
  
  METHODS
  Thirty overweight, nondiabetic, premenopausal women and 28 never overweight controls were included in this analysis. All were participating in a longitudinal investigation of the role of energy metabolism in the etiology of obesity. The overweight women were recruited specifically to have a positive family history of obesity and have a body mass index (BMI) between 27 and 30 kg/m(2) and were studied in the overweight state and after reduction to a normal weight. The never-overweight controls were recruited specifically to have no personal and family history of obesity and were group matched with the weight-reduced post-overweight subjects in terms of premenopausal status, age, BMI, race and sedentary lifestyle.
  
  
  METHODS
  All testing was performed following one month of weight maintenance and during the follicular phase of the menstrual cycle. Hydrostatic weighing was performed to measure body composition and a whole-body maximal oxygen uptake (VO(2max)) test was done to measure aerobic fitness. (31)P MRS was used to determine ATP production from oxidative phosphorylation (OxPhos), 'anaerobic' glycolysis (AnGly), and creatine kinase (CK), as well as muscle metabolic economy. The time constant of ADP (TC(ADP)), V(PCr) (ie the initial rate of PCr resynthesis following exercise), and Q(max) (ie the apparent maximal oxidative ATP production rate) were also calculated as additional markers of mitochondrial function.
  
  
  RESULTS
  Diet-induced weight loss did not have any effects on the anaerobic metabolism markers (AnGly and CK). The aerobic metabolism markers calculated from the initial recovery data (OxPhos and V(PCr)) were unaffected by diet-induced weight loss. However, diet-induced weight loss resulted in improvements in the TC(ADP) and Q(max) in the post-overweight state as compared to their overweight state. There were no differences in any of the anaerobic (AnGly and CK) or oxidative metabolism markers (OxPhos, V(PCr), Q(max) and TC(ADP)) between the post-overweight and control groups.
  
  
  CONCLUSIONS
  Once the overweight women were reduced to a normal-weight state, their skeletal muscle energy metabolism and economy was similar to the never overweight control women. In overweight women, oxidative metabolism or mitochondrial function may be limited by blood flow to the muscle following the cessation of exercise.
authors.affiliation: Department of Critical and Diagnostic Care, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA. newcomer@uab.edu
authors.forename: B R
authors.initials: BR
authors.lastname: Newcomer
authors.forename: D E
authors.initials: DE
authors.lastname: Larson-Meyer
authors.forename: G R
authors.initials: GR
authors.lastname: Hunter
authors.forename: R L
authors.initials: RL
authors.lastname: Weinsier
mesh_terms: D000328:Adult; D015992:Body Mass Index; D016022:Case-Control Studies; D015444:Exercise / Q000502:physiology*; D005260:Female; D006801:Humans; D008137:Longitudinal Studies; D008279:Magnetic Resonance Imaging; D018482:Muscle, Skeletal / Q000098:blood supply / Q000378:metabolism*; D009765:Obesity / Q000209:etiology / Q000378:metabolism*; D010101:Oxygen Consumption; D010759:Phosphorus Isotopes; D010809:Physical Fitness; D011446:Prospective Studies; D015203:Reproducibility of Results; D015431:Weight Loss / Q000502:physiology*
chemical_list: D010759:Phosphorus Isotopes