title: Induction of tumor necrosis factor-alpha by cefodizime in U-937 cells.
abstract: Cefodizime has modulating effects on the release of diverse cytokines. We determined the modulator activity of this antibiotic on the production of TNF in human monocytic U-937 cells. The measurement of TNF was carried out by ELISA test and by a L-929 cells-based citotoxic bioassay. The results showed that cefodizime alone induced the production of TNF on U-937 cells, however, the addition of LPS led to a decrease in the release of this cytokine (p < 0.05). On the other hand, the combination of cefodizime-PMA had a synergic effect (p < 0.05), while addition of LPS to this combination caused a decrease of TNF production (p < 0.05). With these results we conclude that cefodizime modulates the production of TNF in U-937 cells, which is down regulated by the addition of LPS.
authors.affiliation: fguerrai@mailer.main.conacyt.mx
authors.forename: F M
authors.initials: FM
authors.lastname: Guerra-Infante
authors.forename: A
authors.initials: A
authors.lastname: Zamora-Ruíz
authors.forename: M
authors.initials: M
authors.lastname: López-Hurtado
authors.forename: S
authors.initials: S
authors.lastname: Flores-Medina
mesh_terms: D002439:Cefotaxime / Q000031:analogs & derivatives / Q000494:pharmacology*; D002460:Cell Line; D002511:Cephalosporins / Q000494:pharmacology*; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology; D009000:Monocytes / Q000187:drug effects*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
chemical_list: D002511:Cephalosporins; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D002439:Cefotaxime; D013755:Tetradecanoylphorbol Acetate; C033356:cefodizime