The expression of membrane-bound CD14 renders mouse B-1 cells susceptible to LPS.
The surface expression of CD14 on mouse B-1 cells and its role on their response to lipopolysaccharide (LPS) were studied by using the murine TH2.52 B-1 cell line and peritoneal B-1 cells. TH2.52 cells with the B-1 phenotype were found to express membrane-bound CD14. Furthermore, CD14 was expressed on physiological peritoneal CD5+ B-1 cells. The stimulation of CD14-expressing TH2.52 cells with a low concentration of LPS resulted in the activation of nuclear factor (NF)-B and a mitogen-activated protein kinase (MAPK). The LPS-induced NF-B and MAPK activation was markedly inhibited by anti-CD14 antibody. These results suggest that B-1 cells may respond to LPS via membrane-bound CD14.
Sugiyama
T
T
Department of Microbiology and Immunology, and Division of Bacterial Toxin, Research Center for Infectious Disease, Aichi Medical University, Nagakute, Aichi, Japan. sugiyama@aichi-med-u.ac.jp
Koide
N
N
Chakravortty
D
D
Kato
Y
Y
Mu
M M
MM
Yoshida
T
T
Yokochi
T
T
D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000276:immunology; D015870:Gene Expression; D018950:Lipopolysaccharide Receptors / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D008070:Lipopolysaccharides / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C
D018950:Lipopolysaccharide Receptors; D008070:Lipopolysaccharides