title: Aberrant expression of growth factor Wnt-5A in six urinary malignant cell lines.
abstract: OBJECTIVE
  To investigate the expression of growth factor Wnt-5A in urinary tumor cell lines.
  
  
  METHODS
  By semi-quantitative RT-PCR (reverse transcriptase polymerase chain reaction), the expression of Wnt-5A in six urinary malignant cell lines, one primary cultured renal fibroblast and one case of normal human renal tissue was detected using Gel Doc 1000 computer controlled molecular analysis system.
  
  
  RESULTS
  The expression of Wnt-5A in-urinary tumor cell lines was much higher than that in primary cultured renal fibroblasts (PRF) and normal human renal tissue (NRT). The levels of Wnt-5A mRNA were different between six malignant cell lines.
  
  
  CONCLUSIONS
  The overexpression of growth factor Wnt-5A has a potential effect on the oncogenesis of urinary malignancies.
authors.affiliation: Department of Urology, First Hospital, Beijing Medical University, Institute of Urology, Beijing Medical University, Beijing 100034, China.
authors.forename: L
authors.initials: L
authors.lastname: Zhuang
authors.forename: Z
authors.initials: Z
authors.lastname: Zhang
authors.forename: Y
authors.initials: Y
authors.lastname: Guo
mesh_terms: D002292:Carcinoma, Renal Cell / Q000235:genetics / Q000473:pathology; D015870:Gene Expression; D006801:Humans; D007680:Kidney Neoplasms / Q000235:genetics* / Q000473:pathology; D008297:Male; D011471:Prostatic Neoplasms / Q000235:genetics / Q000473:pathology; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D014407:Tumor Cells, Cultured; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000473:pathology; D051153:Wnt Proteins; D000071818:Wnt-5a Protein
chemical_list: D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; C085389:WNT5A protein, human; D051153:Wnt Proteins; D000071818:Wnt-5a Protein