DTNB inhibits calcium response of rat brain cortical slices to anoxia of various duration.
The effects of DTNB on changes in intracellular Ca(2+) content in slices of rat cortex induced by 2- or 10-min anoxia were studied fluorometrically. DTNB (200 microM) prevented excessive intracellular Ca(2+) accumulation during and after 10-min anoxia and moderate increase in Ca(2+) content induced by 2-min anoxia. Our results suggest that redox sites of NMDA receptors participates in both pathogenic and adaptive Ca(2+)-mediated processes activated by anoxia.
Semenov
D G
DG
I. P. Pavlov Institute of Physiology, Russian Academy of Sciences, St. Petersburg; Medical Research Center, Polish Academy of Sciences, Warsaw. anoxia@pavlov.infran.ru
Samoilov
M O
MO
Lazarevich
E V
EV
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002540:Cerebral Cortex / Q000378:metabolism*; D004228:Dithionitrobenzoic Acid / Q000494:pharmacology*; D002534:Hypoxia, Brain / Q000378:metabolism*; D066298:In Vitro Techniques; D017136:Ion Transport / Q000187:drug effects; D010084:Oxidation-Reduction; D051381:Rats; D011921:Rats, Inbred WKY; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism
D016194:Receptors, N-Methyl-D-Aspartate; D004228:Dithionitrobenzoic Acid; D002118:Calcium