A novel affinity chromatography method for the co-purification of deoxycytidine kinase and cytidine deaminase.
By affinity chromatography with Sepharose coupled to 2'-deoxy-1-beta-D-ribofuranosyl-N4-dodecanoylcytosine, deoxycytidine kinase and cytidine deaminase were purified 1,950- and 2,240-fold, respectively, from Ehrlich carcinoma cells, and their enzyme activities for several deoxycytidine analogs were investigated.
Uchida
H
H
Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Fukui University, Japan. uchida@acbio.acbio.fukui-u.ac.jp
Morinaga
H
H
Misaki
T
T
Miyazaki
T
T
Uwajima
T
T
Obata
T
T
Endo
Y
Y
Matsuda
A
A
Sasaki
T
T
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000201:enzymology; D002846:Chromatography, Affinity / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D003564:Cytidine Deaminase / Q000302:isolation & purification* / Q000378:metabolism; D003842:Deoxycytidine Kinase / Q000302:isolation & purification* / Q000378:metabolism; D007700:Kinetics; D051379:Mice; D012685:Sepharose / Q000031:analogs & derivatives / Q000378:metabolism; D014407:Tumor Cells, Cultured
D012685:Sepharose; D003842:Deoxycytidine Kinase; D003564:Cytidine Deaminase