title: Enantioselective analysis of albendazole sulfoxide in cerebrospinal fluid by capillary electrophoresis.
abstract: Albendazole (ABZ) is a benzimidazole anthelmintic drug used in the treatment of neurocysticercosis. After oral administration, ABZ is rapidly oxidized to albendazole sulfoxide (ABZSO), which has an asymmetric sulfur center, and later to albendazole sulfone (ABZSO2). ABZSO is the active metabolite responsible for the therapeutic effect of the drug. Previous studies have demonstrated pharmacokinetic differences between the two enantiomers, with the predominance of (+)-ABZSO in human biological fluids. This article describes for the first time the enantioselective analysis of ABZSO in cerebrospinal fluid (CSF) using capillary electrophoresis. The samples were prepared by liquid-liquid extraction using chloroform:isopropanol (8:2 v/v). The resolution of ABZSO enantiomers was obtained with a fused-silica capillary (60 cm x 75 microm ID) using 20 mmol/L Tris, pH 7.0, with 3.0% w/w sulfated beta-cyclodextrin as running buffer. The coefficient of variations and % relative error obtained for both within-day and between-days assays were lower than 15%. The method was linear over the concentration range of 100 to 2,500 ng/mL for each enantiomer, indicating that it is suitable for the analysis of ABZSO enantiomers in CSF from patients medicated with ABZ.
authors.affiliation: Departamento de Química, Facultade de Filosofia, Ciências e Letras de Ribeirão Preto, Brazil.
authors.forename: F O
authors.initials: FO
authors.lastname: Paias
authors.forename: V L
authors.initials: VL
authors.lastname: Lanchote
authors.forename: O M
authors.initials: OM
authors.lastname: Takayanagui
authors.forename: P S
authors.initials: PS
authors.lastname: Bonato
mesh_terms: D019840:2-Propanol; D015766:Albendazole / Q000031:analogs & derivatives* / Q000134:cerebrospinal fluid*; D000871:Anthelmintics / Q000134:cerebrospinal fluid*; D002725:Chloroform; D003505:Cyclodextrins; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D007202:Indicators and Reagents; D011786:Quality Control; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013237:Stereoisomerism; D047392:beta-Cyclodextrins
chemical_list: D000871:Anthelmintics; D003505:Cyclodextrins; D007202:Indicators and Reagents; D047392:beta-Cyclodextrins; D002725:Chloroform; D015766:Albendazole; C027186:albendazole sulfoxide; C031215:betadex; D019840:2-Propanol