Synergy between ascorbate and alpha-tocopherol on fibroblasts in culture.
Ascorbate and tocopherol are important antioxidants that protect cells against oxidative stress. The interaction of ascorbate and alpha-tocopherol in cells is difficult to detect as both ascorbate and alpha-tocopherol are unstable in vitro in a biological medium. We examined the interactions between human dermal fibroblasts, ascorbate and alpha-tocopherol to determine the effects of the vitamins on growth and cell viability. The interaction of ascorbate and alpha-tocopherol was studied in a fibroblast culture medium during 48h. Ascorbate and alpha-tocopherol were detected by fluorimetry after high-performance liquid chromatography (HPLC). Cell growth and cell viability were studied by cell numeration after trypan blue staining. The ascorbate concentration fell in presence of alpha-tocopherol in cell culture medium under all experimental conditions, with or without cells. Ascorbate partly protected alpha-tocopherol but only in presence of cells. Cell viability was preserved by alpha-tocopherol whereas ascorbate enhanced fibroblast growth. The synergy between ascorbate and alpha-tocopherol corresponds to a consumption of ascorbate which spares alpha-tocopherol but only in presence of cells.
Chepda
T
T
Laboratoire de Biochimie, Faculté de Médecine, Saint-Etienne, France.
Cadau
M
M
Lassabliere
F
F
Reynaud
E
E
Perier
C
C
Frey
J
J
Chamson
A
A
D001205:Ascorbic Acid / Q000032:analysis / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D013050:Spectrometry, Fluorescence; D014810:Vitamin E / Q000032:analysis / Q000494:pharmacology*
D014810:Vitamin E; D001205:Ascorbic Acid