title: Transscleral laser thermotherapy of hamster Greene melanoma: inducing tumour necrosis without scleral damage.
abstract: The feasibility of using transscleral thermotherapy (TSTT) to induce necrosis of choroidal melanoma without causing damage to the sclera was investigated. Fifty-two subcutaneously implanted hamster melanomas covered by human donor sclera were irradiated for 1 min with an 810 nm laser using a 3 mm spot diameter, with and without cooling of the scleral surface. Immediately after irradiation the temperature of the scleral surface was measured with an infrared camera. Irradiation at 2000 mW, without cooling of the sclera, resulted in tumour necrosis to a mean depth of 4.4 mm and a mean diameter of 5.5 mm, without causing damage to the scleral collagen; the surface temperature of the sclera was 44.5 degrees C, and the estimated temperature at the sclera-tumour interface was 60.1 degrees C. There was a sharp demarcation between the viable and the necrotic part of the tumour. TSTT at 2500 mW, without cooling of the sclera, caused maximal tumour necrosis to a mean depth of 5.2 mm and a mean diameter of 5.9 mm; the scleral layers adjacent to the tumour had an estimated temperature of 67.6 degrees C and showed signs of damage in 14% of the experiments. Cooling of the sclera resulted in less thermal damage to the sclera but also less tumour necrosis. Results indicate that TSTT has potential in the treatment of choroidal melanoma.
authors.affiliation: Department of Ophthalmology, Leiden University Medical Centre, PO Box 9600, 2300 RC Leiden, The Netherlands. a.i.rem@ioi.knaw.nl
authors.forename: A I
authors.initials: AI
authors.lastname: Rem
authors.forename: J A
authors.initials: JA
authors.lastname: Oosterhuis
authors.forename: J G
authors.initials: JG
authors.lastname: Korver
authors.forename: T J
authors.initials: TJ
authors.lastname: van den Berg
mesh_terms: D000818:Animals; D006224:Cricetinae; D006801:Humans; D006979:Hyperthermia, Induced / Q000295:instrumentation / Q000379:methods*; D053685:Laser Therapy; D008546:Melanoma, Experimental / Q000473:pathology* / Q000628:therapy*; D009336:Necrosis; D009368:Neoplasm Transplantation; D012590:Sclera / Q000166:cytology*; D012878:Skin Neoplasms / Q000473:pathology* / Q000628:therapy*; D013696:Temperature