An RGDS peptide-binding receptor, FR-1R, localizes to the basal side of the ectoderm and to primary mesenchyme cells in sand dollar embryos.
Immunoblotting using polyclonal antibodies (pAb) raised against an FR-1 receptor (FR-1R), a 57 kDa Arg-Gly-Asp-Ser (RGDS)-binding protein, of the sand dollar Clypeaster japonicus showed that the pAb monospecifically bound to the protein. FR-1R was present in purified plasma membrane, suggesting that the protein is a membrane-bound protein. The molecular structure of FR-1R did not change throughout the early embryogenesis, whereas its expression changed significantly during this period. FR-1R was present in the cortex of unfertilized eggs and was then transferred to the hyaline layer soon after the fertilization. The hyaline layer retained FR-1R immunoreactivity during early embryogenesis. FR-1R appeared on the basal side of the ectoderm at the morula stage and was retained basolaterally, at least, to the early gastrula stage. In mesenchyme blastulae, FR-1R was also present on the surface of primary mesenchyme cells (PMC). FR-1R was localized on the basal side of the ectoderm in early gastrulae, exclusively at the place where PMC formed ventrolateral aggregates, and at the apical tuft ectoderm. In vitro, PMC bound to FR-1R and its binding was inhibited in the presence of a synthetic RGDS peptide or the pAb. The pAb introduced into the blastocoele perturbed PMC migration and gastrulation. FR-1R was weakly recognized by antihuman integrin beta5 subunit pAb.
Katow
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Marine Biological Station, Graduate School of Science, University of Tohoku, Asamushi, Aomori, Aomori 039-3501, Japan. hkatow@mail.cc.tohoku.ac.jp
Sofuku
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D000818:Animals; D000906:Antibodies / Q000378:metabolism; D001665:Binding Sites; D002352:Carrier Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics*; D002448:Cell Adhesion; D002462:Cell Membrane / Q000378:metabolism; D002465:Cell Movement; D004475:Ectoderm / Q000378:metabolism*; D004625:Embryo, Nonmammalian / Q000378:metabolism; D005775:Gastrula; D015151:Immunoblotting; D007150:Immunohistochemistry; D008565:Membrane Proteins; D009842:Oligopeptides / Q000737:chemistry* / Q000378:metabolism*; D011485:Protein Binding; D012617:Sea Urchins; D013997:Time Factors
D000906:Antibodies; D002352:Carrier Proteins; D008565:Membrane Proteins; D009842:Oligopeptides; C447185:RGDS-binding protein, Clypeaster japonicus; C046617:arginyl-glycyl-aspartyl-serine