Mechanoprotection of the plasma membrane in neurons and other non-erythroid cells by the spectrin-based membrane skeleton.
Though the cytomechanics of spectrin have been explored only for erythrocytes, it is thought that the spectrin skeleton acts universally to support the otherwise mechanically vulnerable cell surface bilayer. Evidence for this role is beginning to accumulate and is reviewed here. Compared to that for erythrocytes, cells whose simplicity facilitates biophysical approaches, the evidence is indirect. One way that membrane skeleton/bilayer interactions have been probed is via the behavior of mechanosusceptible ion channels - channel whose gating is perturbed by abnormally high bilayer tension. These initially unresponsive channels become progressively more mechanoresponsive as stretch and chemical reagents damage the membrane skeleton. The straightforward implication is that the intact membrane skeleton is mechanoprotective. In non-erythroid cells there is continual trafficking of bilayer to and from the plasma membrane. Some of the traffic involves spectrin-lined vacuolar membrane. Several lines of evidence suggest that when neurons elongate and remodel their neurites, membrane skeleton-based mechanoprotection allows the dynamic vacuoles and the plasma membrane to participate in mechanosensitive surface area expansion and retrieval.
Morris
C E
CE
Neurosciences, Ottawa Health Research Institute, Ottawa Hospital, 725 Parkdale Ave, Ottawa, Ontario, Canada K1Y 4K9.
D000818:Animals; D001692:Biological Transport; D002462:Cell Membrane / Q000737:chemistry* / Q000378:metabolism* / Q000648:ultrastructure; D003599:Cytoskeleton / Q000737:chemistry / Q000378:metabolism*; D004594:Electrophysiology; D020439:Growth Cones / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D007473:Ion Channels / Q000378:metabolism; D016501:Neurites / Q000378:metabolism; D009474:Neurons / Q000166:cytology* / Q000378:metabolism* / Q000648:ultrastructure; D013049:Spectrin / Q000378:metabolism*; D013314:Stress, Mechanical; D014617:Vacuoles / Q000378:metabolism
D007473:Ion Channels; D013049:Spectrin