Suppressive effect of coffee on lipopolysaccharide-induced hepatitis in D-galactosamine-sensitized rats.
A coffee extract significantly suppressed lipopolysaccharide (LPS)-induced hepatitis in D-galactosamine-sensitized rats, as assessed by the plasma alanine and aspartate aminotransferase activities, when it was added to the diet (30 g/kg) and fed to rats for 14 days. Its effect was as strong as that of a green tea extract. The coffee extract suppressed LPS-induced hepatitis when singly force-fed (1.2 g/kg) 1.5 h prior to the injection of the drugs, whereas a decaffeinated coffee extract had no significant effect. The hepatoprotective effect of caffeine was stronger than that of theobromine. These results indicate that coffee can protect animals from LPS-induced hepatitis, and that the effect of coffee might be mainly due to caffeine.
He
P
P
Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, Japan.
Noda
Y
Y
Sugiyama
K
K
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D002110:Caffeine / Q000032:analysis / Q000627:therapeutic use; D056486:Chemical and Drug Induced Liver Injury / Q000517:prevention & control*; D002851:Chromatography, High Pressure Liquid; D003069:Coffee / Q000737:chemistry; D004032:Diet; D004731:Endotoxins / Q000633:toxicity*; D005688:Galactosamine / Q000633:toxicity*; D008070:Lipopolysaccharides / Q000633:toxicity*; D008297:Male; D010726:Phosphodiesterase Inhibitors / Q000032:analysis / Q000627:therapeutic use; D008517:Phytotherapy; D010936:Plant Extracts / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D013662:Tea / Q000737:chemistry
D003069:Coffee; D004731:Endotoxins; D008070:Lipopolysaccharides; D010726:Phosphodiesterase Inhibitors; D010936:Plant Extracts; D013662:Tea; D002110:Caffeine; C044101:endotoxin, Escherichia coli; D005688:Galactosamine; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase