Non-steroidal anti-inflammatory agent ibuprofen-induced apoptosis, cell necrosis and cell cycle alterations in human leukemic cells in vitro.
The cytotoxic activity of the non-steroidal anti-inflammatory agent ibuprofen to human promyelocytic leukemia cell line HL-60, its multidrug-resistant subline HL-60/VCR (MDR-1 gene coded P-glycoprotein), as well as myeloma U266 and B-lymphoblastoid ARH-77 cell lines was demonstrated with the aid of flow cytometric analysis. Ibuprofen inhibited proliferation and induced apoptosis (detected as sub-G0 nuclei, fluorescein diacetate staining, Annexin-V binding cells and agarose electrophoretic detection of nucleosomal DNA fragmentation) in promyelocytic cells and, to a lesser extent, in U266 and ARH-77 cells.
Jakubíková
J
J
Cancer Research Institute, Slovak Academy of Sciences, Bratislava, Slovak Republic. exonjana@savba.sk
Duraj
T
T
Takácsová
X
X
Hunáková
L
L
Chorváth
B
B
Sedlák
J
J
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology* / Q000627:therapeutic use*; D017209:Apoptosis / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects*; D004587:Electrophoresis, Agar Gel; D005434:Flow Cytometry; D019450:Genes, MDR / Q000187:drug effects; D018922:HL-60 Cells / Q000187:drug effects; D006801:Humans; D007052:Ibuprofen / Q000494:pharmacology* / Q000627:therapeutic use*; D020128:Inhibitory Concentration 50; D007951:Leukemia, Myeloid / Q000188:drug therapy*; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000894:Anti-Inflammatory Agents, Non-Steroidal; D007052:Ibuprofen