title: Developmental and regional distribution of aspartoacylase in rat brain tissue.
abstract: The function of N-acetyl-aspartate (NAA), a predominant molecule in the brain, has not yet been determined. However, NAA is commonly used as a putative marker of viable neurones. To investigate the possible function of NAA, we determined the anatomical, developmental and cellular distribution of aspartoacylase, which catalyses the hydrolysis of NAA. Levels of aspartoacylase activity were measured during postnatal development in several brain regions. The differential distribution of aspartoacylase activity in purified populations of cells derived from the rat CNS was also investigated. The developmental and anatomical distribution of aspartoacylase correlated with the maturation of white matter tracts in the rat brain. Activity increased markedly after 7 days and coincided with the time course for the onset of myelination in the rat brain. Gray matter showed little activity or developmental trend. There was a 60-fold excess in optic nerve (a white matter tract) when compared with cortex at 21 days of development. In the adult brain there was a 18-fold difference in corpus callosum compared with cortex (stripped of corpus callosum). Cellular studies demonstrated that purified cortical neurons and cerebellar granular neurones have no activity. Primary O-2A progenitor cells had moderate activity, with three-fold higher activity in immature oligodendrocyte and 13-fold increase in mature oligodendrocytes (myelinating cells of the CNS). The highest activity was seen in type-2 astrocytes (20-fold difference compared with O-2A progenitors) derived from the same source. Aspartoacylase activity increased with time in freshly isolated astrocytes, with significantly higher activity after 15 days in culture. We conclude that aspartoacylase activity in the developing postnatal brain corresponds with maturation of myelination, and that the cellular distribution is limited to glial cells.
authors.affiliation: MRC Biochemical and Clinical Magnetic Resonance Unit, Department of Biochemistry, University of Oxford, South Parks Road, Oxford. K.Bhakoo@bioch.ox.ac.uk
authors.forename: K K
authors.initials: KK
authors.lastname: Bhakoo
authors.forename: T J
authors.initials: TJ
authors.lastname: Craig
authors.forename: P
authors.initials: P
authors.lastname: Styles
mesh_terms: D000581:Amidohydrolases / Q000032:analysis* / Q000378:metabolism; D000818:Animals; D001224:Aspartic Acid / Q000378:metabolism; D001253:Astrocytes / Q000201:enzymology; D001921:Brain / Q000201:enzymology* / Q000254:growth & development*; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology; D002540:Cerebral Cortex / Q000166:cytology; D007150:Immunohistochemistry; D009474:Neurons / Q000201:enzymology; D051381:Rats; D014018:Tissue Distribution
chemical_list: D001224:Aspartic Acid; D000581:Amidohydrolases; C019751:aspartoacylase