Flow cytometric DNA measurements in squamous cell carcinoma of the vulva: an important prognostic method.
One hundred and thirty-one squamous cell carcinomas of the vulva were examined by FCM-DNA measurements. Samples were prepared from paraffin-embedded tissue. Of these, 66 were found to be diploid, 52 aneuploid and 13 could not be evaluated. The 5-year crude survival rate was 62% for the diploid and 23% for the aneuploid tumors (P < 0.001). The aneuploid tumors without lymph node (LN) metastases showed a 5-year cancer-related survival rate of 44% as compared to 58% for the diploid tumors with LN metastases. In a multivariate Cox regression analysis the most important independent prognostic parameters were (1) LN involvement (P < 0.0001), (2) tumor ploidy (P = 0.0001) and (3) tumor size (P = 0.0039). By using ploidy and lymph node involvement in this way as prognostic factors we are able to identify high- and low-risk groups of patients. We strongly believe that these results should lead to a different attitude towards therapy in vulva cancer patients.
Kaern
J.
J
Departments of Gynecologic Oncology, Tissue culture and Pathology, The Norwegian Radium Hospital, Montebello, 0310 Oslo 3, Norway.
Iversen
T.
T
Tropé
C.
C
Pettersen
E.O.
EO
Nesland
J.M.
JM