title: PSD95 protein level rises in murine somatosensory cortex after sensory training.
abstract: Proteins of the postsynaptic density are implicated in mechanisms of synaptic plasticity. We examined involvement of PSD95 and alphaCaMkII in learning-dependent plastic changes of representational maps in somatosensory cortex of mice. The barrel cortex of mice was examined following a 3 day long classical conditioning training, in which activation of facial vibrissae was linked to an aversive stimulus. This procedure produced expansion of cortical representations of vibrissae involved in the training. In subcellular fraction enriched in postsynaptic densities from the barrel cortex, it was estimated by Western blotting that the level of PSD95 increased after the training by about 50%, while the level of CaMkII remained unchanged. The results indicate involvement of PSD95 in learning-dependent cortical plasticity.
authors.affiliation: Department of Neurophysiology, Nencki Institute, 3 Pasteur St., 02-093 Warsaw, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Skibinska
authors.forename: M
authors.initials: M
authors.lastname: Lech
authors.forename: M
authors.initials: M
authors.lastname: Kossut
mesh_terms: D000344:Afferent Pathways / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D015153:Blotting, Western; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000378:metabolism; D003847:Deoxyglucose / Q000493:pharmacokinetics; D000076122:Disks Large Homolog 4 Protein; D005260:Female; D051528:Guanylate Kinases; D047908:Intracellular Signaling Peptides and Proteins; D007858:Learning / Q000502:physiology*; D008297:Male; D008565:Membrane Proteins; D051379:Mice; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009473:Neuronal Plasticity / Q000502:physiology*; D010812:Physical Stimulation; D013003:Somatosensory Cortex / Q000166:cytology / Q000378:metabolism*; D013347:Subcellular Fractions / Q000378:metabolism; D013570:Synaptic Membranes / Q000378:metabolism*; D015854:Up-Regulation / Q000502:physiology; D014738:Vibrissae / Q000502:physiology*
chemical_list: D000076122:Disks Large Homolog 4 Protein; C501447:Dlg4 protein, mouse; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; C033652:postsynaptic density proteins; D003847:Deoxyglucose; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D051528:Guanylate Kinases