Recombineering: a powerful new tool for mouse functional genomics.
Highly efficient phage-based Escherichia coli homologous recombination systems have recently been developed that enable genomic DNA in bacterial artificial chromosomes to be modified and subcloned, without the need for restriction enzymes or DNA ligases. This new form of chromosome engineering, termed recombinogenic engineering or recombineering, is efficient and greatly decreases the time it takes to create transgenic mouse models by traditional means. Recombineering also facilitates many kinds of genomic experiment that have otherwise been difficult to carry out, and should enhance functional genomic studies by providing better mouse models and a more refined genetic analysis of the mouse genome.
Copeland
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Jenkins
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Court
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D000818:Animals; D001426:Bacterial Proteins / Q000502:physiology; D017101:Bacteriophage P1 / Q000235:genetics; D010582:Bacteriophage lambda / Q000235:genetics; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics; D027042:Chromosomes, Artificial, P1 Bacteriophage / Q000235:genetics; D018244:Chromosomes, Artificial, Yeast / Q000235:genetics; D003001:Cloning, Molecular / Q000379:methods; D004260:DNA Repair; D004269:DNA, Bacterial / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics; D004274:DNA, Recombinant / Q000235:genetics; D004277:DNA, Single-Stranded / Q000235:genetics; D004268:DNA-Binding Proteins; D004926:Escherichia coli / Q000235:genetics; D029968:Escherichia coli Proteins; D043211:Exodeoxyribonuclease V; D005090:Exodeoxyribonucleases / Q000502:physiology; D005544:Forecasting; D015967:Gene Expression Regulation, Viral; D005818:Genetic Engineering / Q000379:methods*; D023281:Genomics / Q000379:methods*; D051379:Mice / Q000235:genetics*; D018345:Mice, Knockout; D008822:Mice, Transgenic; D011938:Rec A Recombinases / Q000378:metabolism; D011995:Recombination, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D012441:Saccharomyces cerevisiae / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D019076:Transgenes; D014764:Viral Proteins / Q000502:physiology
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004271:DNA, Fungal; D004274:DNA, Recombinant; D004277:DNA, Single-Stranded; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; C084497:RecT protein, E coli; D014764:Viral Proteins; D011938:Rec A Recombinases; D005090:Exodeoxyribonucleases; C038225:recE protein, E coli; D043211:Exodeoxyribonuclease V