[Analysis of paeonol in Paeonia suffruticosa Andr. and its patent preparations by supercritical fluid extraction and capillary gas chromatography].
A supercritical fluid extraction (SFE) method was developed for the extraction of paeonol in Paeonia suffruticosa Andr. and its patent preparations. A systematic method was used to optimize five variables (temperature, pressure, modifier concentration, static extracting time and CO2 dynamic extracting volume) of SFE. Sample extracts were analyzed by wide-bore capillary gas chromatography (CGC). The method showed good linearity (gamma = 0.9999) in the range of 0.1-1.6 mg.ml-1 of paeonol. The average recovery was 97.8% with RSD = 2.35% (n = 3) for Paeonia suffruticosa andr., 100.3% with RSD = 1.89% (n = 3) for its patent preparations.
Miao
H J
HJ
Changzheng Hospital, Second Military Medical University, Shanghai 200003.
Liu
Z L
ZL
Li
Y H
YH
D000098:Acetophenones / Q000032:analysis*; D002849:Chromatography, Gas / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D019684:Magnoliopsida / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*
D000098:Acetophenones; D004365:Drugs, Chinese Herbal; C013638:paeonol