title: Human HSP 60 peptide responsive T cell lines are similarly present in both Behçet's disease patients and healthy controls.
abstract: Heat shock protein (HSP, 60/65 kDa) is investigated as a candidate autoantigen in Behçet's disease (BD), a systemic vasculitis of unknown origin, and a prominent response to 'disease-specific epitopes' of mycobacterial and human HSP60/65 is described in BD patients. In this study, long-term T cell lines from peripheral blood of BD patients (n=6) and controls (n=7) were stimulated with mycobacterial recombinant HSP and purified protein derivate (PPD) and expanded with IL-2. In the BD group, 15 out 27 and in the controls, 25 out of 35 PPD specific T cell lines have responded to the synthetic peptides of the human HSP60. Out of the primarily HSP-specific T cell lines, 17/23 in patients and 8/8 in controls did recognize a peptide of human origin. T cell lines specifically reactive to 136-150, 179-197, 244-258 and 336-351 could be raised with similar frequency in both groups. In contrast to a previous report, T cells also reacted to peptide 425-441 frequently in both groups. The results demonstrate that the human proliferative response to mycobacterial HSP may also target the self-protein in both BD patients and controls. However, the responsive T cells may have different effects depending on their functional features such as cytokine secretions.
authors.affiliation: Department of Physiology, Istanbul Medical Faculty, Istanbul University, Istanbul, Turkey. gsaruhan@istanbul.edu.tr
authors.forename: G
authors.initials: G
authors.lastname: Saruhan-Direskeneli
authors.forename: B
authors.initials: B
authors.lastname: Celet
authors.forename: E
authors.initials: E
authors.lastname: Eksioglu-Demiralp
authors.forename: H
authors.initials: H
authors.lastname: Direskeneli
mesh_terms: D000328:Adult; D000942:Antigens, Bacterial / Q000276:immunology; D001324:Autoantigens / Q000276:immunology; D001528:Behcet Syndrome / Q000235:genetics / Q000276:immunology*; D002460:Cell Line; D018834:Chaperonin 60 / Q000276:immunology*; D003429:Cross Reactions / Q000276:immunology; D018984:Epitopes, T-Lymphocyte / Q000276:immunology; D005260:Female; D005434:Flow Cytometry; D006684:HLA-DR Antigens / Q000276:immunology; D006801:Humans; D016130:Immunophenotyping; D008297:Male; D008875:Middle Aged; D009161:Mycobacterium / Q000276:immunology; D011994:Recombinant Proteins / Q000276:immunology; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology*
chemical_list: D000942:Antigens, Bacterial; D001324:Autoantigens; D018834:Chaperonin 60; D018984:Epitopes, T-Lymphocyte; D006684:HLA-DR Antigens; D011994:Recombinant Proteins