Comparison of cellular composition of induced sputum analyzed with Wright staining and immunocytochemistry.
We sought to compare cell counts based on cellular morphology and obtained after Wright staining with cell counts obtained after immunocytochemistry in induced sputum. Counts of eosinophils, neutrophils, macrophages, lymphocytes, and epithelial cells identified after Wright staining were compared with the counts obtained after immunocytochemistry through use of mAbs in 25 samples. Agreement between Wright staining and immunocytochemistry was poor for lymphocytes and epithelial cells. Rather than Wright staining, immunocytochemistry should be used to accurately identify lymphocytes and epithelial cells.
Lemière
C
C
Department of Chest Medicine, Hôpital du Sacré-Coeur, Montral, Quebec, Canada.
Taha
R
R
Olivenstein
R
R
Hamid
Q
Q
D001249:Asthma / Q000473:pathology*; D002452:Cell Count / Q000379:methods*; D006801:Humans; D007150:Immunohistochemistry; D009784:Occupational Diseases / Q000473:pathology*; D013183:Sputum / Q000166:cytology*; D013194:Staining and Labeling