title: Purification of engineered human pancreatic ribonuclease.
authors.affiliation: Laboratori d'Enginyeria de Proteïnes, Departament de Biologia, Universitat de Girona, E-17071 Girona, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Ribó
authors.forename: A
authors.initials: A
authors.lastname: Benito
authors.forename: A
authors.initials: A
authors.lastname: Canals
authors.forename: M V
authors.initials: MV
authors.lastname: Nogués
authors.forename: C M
authors.initials: CM
authors.lastname: Cuchillo
authors.forename: M
authors.initials: M
authors.lastname: Vilanova
mesh_terms: D002852:Chromatography, Ion Exchange / Q000379:methods; D002942:Circular Dichroism; D006801:Humans; D007700:Kinetics; D010084:Oxidation-Reduction; D011487:Protein Conformation; D011489:Protein Denaturation; D015202:Protein Engineering; D017510:Protein Folding; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D012259:Ribonuclease, Pancreatic / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D013379:Substrate Specificity
chemical_list: D011994:Recombinant Proteins; D012259:Ribonuclease, Pancreatic