title: Effects of antecedent fermentative and respiratory growth on the detection of chloramine-stressed Escherichia coil and Salmonella typhimurium.
abstract: In vitro laboratory studies were performed to assess the effects of antecedent growth conditions on the recovery of Escherichia coli ATCC 25922 and Salmonella typhimurium ATCC 14028 following chloramine disinfection. Six- and 18-h cultures of each organism were grown under aerobic, fermentative, and nitrate-reducing conditions prior to disinfection. At predetermined time intervals during a 10-min exposure to chloramine, survivors were surface plated on nonselective recovery media to determine C(n)t values. It was observed that nitrate-reducing growth predisposed the test organisms towards an increased sensitivity to chloramine stress over cells grown under fermentation or aerobic conditions (p < 0.01).
authors.affiliation: Division of Plant and Soil Sciences, West Virginia University, Morgantown 26506-6057, USA.
authors.forename: R L
authors.initials: RL
authors.lastname: Thunberg
authors.forename: A J
authors.initials: AJ
authors.lastname: Sexstone
authors.forename: J P
authors.initials: JP
authors.lastname: Calabrese
authors.forename: G K
authors.initials: GK
authors.lastname: Bissonnette
mesh_terms: D000332:Aerobiosis; D002700:Chloramines / Q000494:pharmacology*; D015169:Colony Count, Microbial; D003470:Culture Media; D004203:Disinfection / Q000379:methods; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development*; D005285:Fermentation; D009566:Nitrates / Q000378:metabolism; D010084:Oxidation-Reduction; D010101:Oxygen Consumption; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000254:growth & development*
chemical_list: D002700:Chloramines; D003470:Culture Media; D009566:Nitrates; C030816:chloramine