Isolation and characterization of barley lipid transfer protein and protein Z as beer allergens.
Beer has recently been implicated as the causative agent of contact urticaria and severe IgE-mediated anaphylaxis. However, no allergen from beer has as yet been isolated and characterized. Two major components of 45 kd and 9 kd were detected in crude protein preparations from beer. Both components were purified; they were identified as barley protein Z(4) (45 kd) and lipid transfer protein 1 (LTP1; 9 kd). Protein Z(4) was recognized by the 4 individual sera tested but provoked weak positive responses to skin testing in 2 of 4 beer-allergic patients. Purified LTP1 showed reactivity with 3 of 4 individual sera and induced strong positive skin prick responses in all 4 patients tested. Barley LTP1 and protein Z(4) have been identified as the main beer allergens.
García-Casado
G
G
Unidad de Bioquímica, Departamento de Biotecnología, E.T.S. Ingenieros Agrónomos, Madrid, Spain.
Crespo
J F
JF
Rodríguez
J
J
Salcedo
G
G
D000328:Adult; D000485:Allergens / Q000276:immunology* / Q000302:isolation & purification; D001515:Beer / Q000009:adverse effects*; D002352:Carrier Proteins / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D050556:Fatty Acid-Binding Proteins; D001467:Hordeum / Q000737:chemistry / Q000276:immunology*; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000209:etiology / Q000276:immunology*; D010940:Plant Proteins / Q000276:immunology* / Q000302:isolation & purification; D012882:Skin Tests
D000485:Allergens; D002352:Carrier Proteins; D050556:Fatty Acid-Binding Proteins; C422456:Ltp1 protein, barley; D010940:Plant Proteins; C119298:protein Z, Avena fatua