title: Toxicity of domoic acid in the marine mussel Mytilus edulis.
abstract: The neurotoxic, immunotoxic and genotoxic effects of domoic acid (DA) on the blue mussel Mytilus edulis were investigated by biomarkers, acethylcholinesterase (ChE) activity in gills, DNA fragmentation in digestive glands, vitality and phagocytosis activity of haemocytes in haemolymph of mussels. After intra muscular injection of DA at the concentrations ranging from 1-500 ng/g body weight (bw), no neurotoxic effect was detected within incubation times of 48 h and 7 d. The vitality of haemocytes remained in all mussels at the level of control samples within 48 h, and increased significantly after 7 d (P<0.05). At DA concentrations ranging from 1 to 100 ng/g bw haemocytes suggested a great phagocytosis activity, but no alteration in their number by both incubation times. By increasing DA concentration of 500 ng/g bw, the number of haemocytes doubled in 48 h without any change in phagocytosis activity. Primary DNA lesions in digestive glands of all injected mussels were determined in acute phase of poisoning within 48 h, and rapidly repaired after 7 d of incubation.
authors.affiliation: Department of Ecotoxicology, Institute for Ecological Research and Technology, Technical University of Berlin, 14195 Berlin, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Dizer
authors.forename: B
authors.initials: B
authors.lastname: Fischer
authors.forename: A S
authors.initials: AS
authors.lastname: Harabawy
authors.forename: M C
authors.initials: MC
authors.lastname: Hennion
authors.forename: P D
authors.initials: PD
authors.lastname: Hansen
mesh_terms: D000818:Animals; D049872:Bivalvia / Q000276:immunology*; D002802:Cholinesterases / Q000187:drug effects; D053938:DNA Fragmentation / Q000187:drug effects; D005880:Gills / Q000378:metabolism; D016905:Gram-Negative Bacterial Infections / Q000276:immunology / Q000662:veterinary*; D006434:Hemocytes / Q000187:drug effects; D007107:Immune System / Q000187:drug effects*; D007608:Kainic Acid / Q000031:analogs & derivatives* / Q000633:toxicity*; D009467:Neuromuscular Depolarizing Agents / Q000633:toxicity*; D010587:Phagocytosis / Q000187:drug effects; D012440:Saccharomyces / Q000276:immunology
chemical_list: D009467:Neuromuscular Depolarizing Agents; D002802:Cholinesterases; C012301:domoic acid; D007608:Kainic Acid