Post-antibiotic effect of the antimicrobial peptide lactoferricin on Escherichia coli and Staphylococcus aureus.
Lactoferricin is an antimicrobial peptide generated by gastric pepsin cleavage of lactoferrin. A possible post-antibiotic effect (PAE) of bovine lactoferricin (Lfcin B) and two shorter peptide derivatives against Staphylococcus aureus and Escherichia coli was studied. A drug removal technique involving centrifugation and washing was used. No PAE was found for Lfcin B against these two bacteria. The shorter derivatives had a short PAE against E. coli. They had a short negative PAE against S. aureus. In conclusion, the overall PAE is not overwhelming, but the small differences found between the different peptide-bacteria combinations could indicate that different peptide mechanisms of action might be present.
Haukland
H H
HH
Department of Medical Microbiology, University Hospital of Tromsø, N-9038 Tromsø, Norway. mlabhh@rito.no
Vorland
L H
LH
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002417:Cattle; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development*; D006801:Humans; D007781:Lactoferrin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D020539:Sequence Analysis, Protein; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000254:growth & development*
D000900:Anti-Bacterial Agents; D010455:Peptides; C082171:lactoferricin B; D007781:Lactoferrin