Hepatocyte attachment onto thermosensitive poly(N-isopropylacrylamide-co-N-p-vinylbenzyl-O-beta-D-galactopyranosyl-(1 --> 4)-D-gluconamide).
Temperature sensitive poly(N-isopropylacrylamide) (PIPAAM) was incorporated into hepatocyte-recognizable poly[N-p-vinylbenzyl-O-beta-D-galactopyranosyl-(1 --> 4)-D-gluconamide] (PVLA) for thermal modulating of hepatocyte attachment. The copolymer, poly(N-isopropylacrylamide-co-N-p-vinylbenzyl-O-beta-D-galactopyranosyl-(1 --> 4)-D-gluconamide) (abbreviated as [P(IPAAM-co-VLA)] (PIPAAM/PVLA = 9/1 in mol%) exhibited lower critical solution temperature (LCST) at 34 degrees C and also showed very good hepatocytes-recognizablility through the specific interaction between asialoglycoprotein receptors on the cell surfaces and galactose moiety of the copolymer. The cells attached on this copolymer were easily detached by lowering the temperature below the LCST of the copolymer. Morphological damage of the detached cell was not observed.
Goto
M
M
BioQuest Research Ltd., Tokyo, Japan.
Makino
Y
Y
Kobayashi
K
K
Cho
C S
CS
Akaike
T
T
D000180:Acrylic Resins / Q000138:chemical synthesis / Q000737:chemistry / Q000493:pharmacokinetics*; D000818:Animals; D001665:Binding Sites; D001672:Biocompatible Materials / Q000737:chemistry / Q000493:pharmacokinetics; D002448:Cell Adhesion; D004187:Disaccharides / Q000138:chemical synthesis / Q000737:chemistry / Q000493:pharmacokinetics*; D005260:Female; D022781:Hepatocytes / Q000737:chemistry / Q000166:cytology*; D007785:Lactose / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry / Q000493:pharmacokinetics*; D009682:Magnetic Resonance Spectroscopy; D008562:Membrane Glycoproteins / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D008858:Microscopy, Phase-Contrast; D011137:Polystyrenes / Q000138:chemical synthesis / Q000737:chemistry / Q000493:pharmacokinetics*; D011485:Protein Binding; D051381:Rats; D017207:Rats, Sprague-Dawley; D013696:Temperature; D014753:Vinyl Compounds / Q000138:chemical synthesis / Q000737:chemistry / Q000493:pharmacokinetics*
D000180:Acrylic Resins; D001672:Biocompatible Materials; D004187:Disaccharides; D008562:Membrane Glycoproteins; D011137:Polystyrenes; D014753:Vinyl Compounds; C454700:poly(N-isopropylacrylamide-co-N-p-vinylbenzyl-O-galactopyranosyl-(1-4)-gluconamide); C052970:poly-N-isopropylacrylamide; C074112:poly-N-4-vinylbenzyllactonamide; D007785:Lactose