[Persistence of Escherichia coli recombinant strains in experimental animals].
Recombinant E. coli strains, obtained by gene engineering techniques and capable of producing human alpha-interferon and HIV proteins, were studied. The cultures under study were completely eliminated from the body of experimental animals (mice) in 48 hours, and generalization of the infectious process took place. The study revealed that these recombinant strains had low virulence and were weakly adhesive, nontoxigenic and weakly toxic. Thus, the recombinant strains under study could be classified with class 3-4 of danger according to the "Classification of Strains of Industrial Microorganisms by the Degree of Their Danger".
Vorob'ev
A A
AA
Sechenov Medical Academy Moscow, Russia.
Khoroshko
N V
NV
D000372:Agglutination Tests; D000818:Animals; D000942:Antigens, Bacterial / Q000096:biosynthesis; D001717:Birds; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity*; D006168:Guinea Pigs; D006801:Humans; D007370:Interferon Type I / Q000096:biosynthesis; D007928:Lethal Dose 50; D051379:Mice; D011994:Recombinant Proteins; D011995:Recombination, Genetic; D014774:Virulence
D000942:Antigens, Bacterial; D007370:Interferon Type I; D011994:Recombinant Proteins