Acanthopanax senticosus root inhibits mast cell-dependent anaphylaxis.
BACKGROUND
Mast cells synthesize and secrete chemical mediators which play a central role in anaphylaxis.


METHODS
The effect of Acanthopanax senticosus root (ASR) on mast cell-dependent anaphylaxis was investigated.


RESULTS
ASR inhibited compound 48/80-induced systemic anaphylactic shock at the dose of 1.0 g/kg by 50%. When ASR was given as pre-treatment at concentrations ranging from 0.01 to 2.0 g/l, the histamine release from rat peritoneal mast cells induced by compound 48/80 was reduced in a dose-dependent manner. ASR (2.0 g/kg) also inhibited passive cutaneous anaphylaxis activated by anti-dinitrophenyl (DNP) IgE to 53.17+/-6.62%. Moreover, ASR inhibited tumor necrosis factor-alpha production in a concentration-dependent manner, and the treatment of 1 g/l blocked the production by 32.5+/-3.50% compared to saline value.


CONCLUSIONS
ASR may possess effective anti-anaphylactic activity.
Yi
J M
JM
Department of Oriental Pharmacy, College of Pharmacy and Center of Oriental Medicinal Science, Wonkwang University, Chonbuk, 570-749, Iksan City, South Korea.
Kim
M S
MS
Seo
S W
SW
Lee
K N
KN
Yook
C S
CS
Kim
H M
HM
D000707:Anaphylaxis / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D000818:Animals; D002478:Cells, Cultured; D004136:Dinitrobenzenes / Q000276:immunology; D006632:Histamine / Q000378:metabolism; D007073:Immunoglobulin E / Q000494:pharmacology; D008297:Male; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D010936:Plant Extracts / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal; D051381:Rats; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D003189:p-Methoxy-N-methylphenethylamine / Q000009:adverse effects
D004136:Dinitrobenzenes; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D007073:Immunoglobulin E; D003189:p-Methoxy-N-methylphenethylamine; D006632:Histamine