Oral low-carbohydrate alcohol liquid diet induces experimental steatohepatitis in the rat.
The intragastric tube feeding model of alcoholic liver disease in the rat induces significant liver histopathology, including steatohepatitis and fibrosis. The question is, if the same low-carbohydrate diet is fed ad lib, will the same pathology develop? Rats were fed a liquid diet with ethanol ad lib that was low in calories derived from carbohydrates for 2 months. The urinary ethanol levels (UALs) were monitored at hourly, daily, and weekly intervals, and the growth of the rats was charted. The liver histopathology and blood transaminase levels were determined. Rats fed ethanol grew 1 g/day, which was 2 g/day less than when they were fed the same diet intragastrically. UALs varied hourly between 150 and 500 mg%, daily between 120 and 360 mg%, and weekly between 0 and 500 mg%. Individual rat UALs showed no predictable pattern. The pair-fed controls ate all of their daily ration within 12 h, then fasted until the next day. The histopathology and blood alanine aminotransferase were similar to those seen with the intragastric tube feeding of the same diet, except that necrosis, inflammation, and fibrosis did not develop. The conclusion was that the oral feeding of a low-carbohydrate diet produces less liver injury than that produced by the same diet fed intragastrically. The UALs varied hourly, daily, and weekly in individual rats, making it difficult to synchronize UALs at the time of sacrifice.
Li
J
J
Department of Pathology, Harbor-UCLA Medical Center, Torrance, California 90509, USA.
French
B A
BA
Riley
N
N
Bardag-Gorce
F
F
Fu
P
P
French
S W
SW
D000284:Administration, Oral; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D019392:Cytochrome P-450 CYP2E1 / Q000378:metabolism; D004040:Dietary Carbohydrates / Q000008:administration & dosage*; D004789:Enzyme Activation / Q000187:drug effects; D000431:Ethanol / Q000008:administration & dosage* / Q000097:blood / Q000652:urine; D006519:Hepatitis, Alcoholic / Q000201:enzymology / Q000209:etiology* / Q000473:pathology; D007124:Immunoenzyme Techniques; D008099:Liver / Q000201:enzymology; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
D004040:Dietary Carbohydrates; D000431:Ethanol; D019392:Cytochrome P-450 CYP2E1