Human immunodeficiency virus type 1 (HIV-1) non-B subtypes are similar to HIV-1 subtype B in that coreceptor specificity is a determinant of cytopathicity in human lymphoid tissue infected ex vivo.
We sought to determine the relationship between virus-mediated CD4(+) T-lymphocyte cytopathicity and viral coreceptor preference among various human immunodeficiency virus type 1 (HIV-1) subtypes in an ex vivo-infected human lymphoid tissue model. Our data show that all R5 HIV-1 infections resulted in mild depletion of CD4(+) T lymphocytes, whereas all X4 HIV-1 infections caused severe depletion of CD4(+) T lymphocytes regardless of their subtype origin. Thus, at least for the viruses within subtypes A, B, C, and E that were tested, coreceptor specificity is a critical factor that determines the ability of HIV-1 to deplete CD4(+) T cells in human lymphoid tissue infected ex vivo.
Malkevich
N
N
Laboratory of Cellular and Molecular Biophysics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA.
Womack
C
C
Pandya
P
P
Grivel
J C
JC
Fauci
A S
AS
Margolis
L
L
D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology / Q000821:virology; D018791:CD4 Lymphocyte Count; D015497:HIV-1 / Q000145:classification* / Q000472:pathogenicity; D006801:Humans; D008221:Lymphoid Tissue / Q000821:virology*; D019713:Receptors, CCR5 / Q000502:physiology*; D019718:Receptors, CXCR4 / Q000502:physiology*
D019713:Receptors, CCR5; D019718:Receptors, CXCR4