Suppression subtraction hybridization and northern analysis reveal upregulation of heat shock, trkB, and sodium calcium exchanger genes following global cerebral ischemia in the rat.
Driver (sham-operated) and tester (ischemic) hippocampal cDNAs were subtracted, and the resulting ischemia-induced upregulated gene expression was verified by northern analysis. cDNAs isolated corresponded to (1) genes known to be upregulated following ischemia, (hsc70, hsp90, hsp105 and trkB) and (2) a gene not previously implicated with cerebral ischemia, sodium calcium exchanger (ncx). Furthermore, upregulation of these genes was demonstrated following preconditioning transient global ischemia.
Majda
B T
BT
Department of Neurosurgery, Sir Charles Gairdner Hospital, the University of Western Australia, Nedlands, Australia.
Meloni
B P
BP
Rixon
N
N
Knuckey
N W
NW
D000818:Animals; D015152:Blotting, Northern; D002545:Brain Ischemia / Q000235:genetics / Q000378:metabolism*; D018076:DNA, Complementary / Q000235:genetics; D020869:Gene Expression Profiling / Q000379:methods*; D005786:Gene Expression Regulation; D006360:Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics; D006624:Hippocampus / Q000378:metabolism*; D002546:Ischemic Attack, Transient / Q000235:genetics / Q000378:metabolism; D008297:Male; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D020813:Receptor, trkB / Q000096:biosynthesis* / Q000235:genetics; D019831:Sodium-Calcium Exchanger / Q000096:biosynthesis* / Q000235:genetics; D013382:Subtraction Technique; D015854:Up-Regulation
D018076:DNA, Complementary; D006360:Heat-Shock Proteins; D009419:Nerve Tissue Proteins; D019831:Sodium-Calcium Exchanger; D020813:Receptor, trkB