title: The use of polymerase chain reaction assay to diagnose proliferative gill disease in channel catfish (Ictalurus punctatus).
abstract: To assess the potential of a polymerase chain reaction (PCR) assay as a diagnostic tool in the detection of proliferative gill disease (PGD) in channel catfish (Ictalurus punctatus), PCR assays were compared with the traditional diagnostic methods of gill wet mounts and histology. A PCR assay using primers for Aurantiactinomyxon ictaluri, the actinospore associated with PGD, was performed with tissues from fish from commercial ponds. Using histology as the "gold standard," the sensitivity, specificity, and accuracy of the PCR assay were all >90%. In comparison, the wet mount examinations had a lower sensitivity and specificity. Using the chi-square test and a test for strength of association, there was a significant, strong association between results obtained by PCR and those obtained by the other 2 methods. These results demonstrate that the PCR assay is a good diagnostic tool for the detection of PGD.
authors.affiliation: College of Veterinary Medicine, Mississippi State University, Mississippi State 39762, USA.
authors.forename: J W
authors.initials: JW
authors.lastname: Whitaker
authors.forename: L M
authors.initials: LM
authors.lastname: Pote
authors.forename: L
authors.initials: L
authors.lastname: Khoo
authors.forename: R
authors.initials: R
authors.lastname: Shivaji
authors.forename: L A
authors.initials: LA
authors.lastname: Hanson
mesh_terms: D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D005393:Fish Diseases / Q000175:diagnosis* / Q000235:genetics / Q000469:parasitology; D005880:Gills / Q000469:parasitology* / Q000473:pathology; D007059:Ictaluridae / Q000469:parasitology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D017931:DNA Primers