A combination of roxithromycin and imipenem as an antimicrobial strategy against biofilms formed by Staphylococcus aureus.
We examined the effects of a combination of roxithromycin and imipenem on a biofilm model of Staphylococcus aureus. Treatment with roxithromycin alone and imipenem alone did not decrease the number of viable bacterial cells compared with the control. However, a combination treatment of roxithromycin and imipenem significantly decreased the number of viable bacterial cells on day 8 after inoculation in the in vivo model (P < 0.01). On days 5 and 8 after inoculation, numerous polymorphonuclear leucocytes and macrophages invaded the bacterial clusters in the roxithromycin- and roxithromycin/imipenem-treated groups, but did not invade the control or imipenem-treated groups. The present study indicated that a combination of roxithromycin and imipenem is a potentially effective treatment for S. aureus biofilm-associated skin infections as it can induce the invasion of polymorphonuclear leucocytes into the biofilm.
Yamasaki
O
O
Department of Dermatology, Okayama University Medical School, 2-5-1 Shikata-cho, Okayama, 700-8558, Japan. kota616@cc.okayama-u.ac.jp
Akiyama
H
H
Toi
Y
Y
Arata
J
J
D000818:Animals; D000900:Anti-Bacterial Agents / Q000627:therapeutic use*; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D004195:Disease Models, Animal; D004359:Drug Therapy, Combination; D006801:Humans; D015378:Imipenem / Q000627:therapeutic use*; D051379:Mice; D015575:Roxithromycin / Q000627:therapeutic use*; D013207:Staphylococcal Skin Infections / Q000188:drug therapy / Q000382:microbiology; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000254:growth & development; D013845:Thienamycins / Q000627:therapeutic use*; D016896:Treatment Outcome
D000900:Anti-Bacterial Agents; D013845:Thienamycins; D015575:Roxithromycin; D015378:Imipenem