Protein-fold evolution in the test tube.
Currently, the combination of library selection and directed evolution is the most powerful approach for finding proteins with novel folds or functions. In the past, most studies concentrated either on protein scaffolds with a given fold or on short peptides. With the recent development of potent in vitro selection and evolution techniques, the screening of much larger sequence space is possible, allowing for the de novo generation of proteins.
Schaffitzel
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Biochemisches Institut, Winterthurerstr. 190, CH-8057 Zürich, Switzerland.
Plückthun
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D000255:Adenosine Triphosphate / Q000378:metabolism; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D019020:Directed Molecular Evolution; D066298:In Vitro Techniques; D019151:Peptide Library; D017510:Protein Folding
D002352:Carrier Proteins; D019151:Peptide Library; D000255:Adenosine Triphosphate