title: Interleukin-17 and lung host defense against Klebsiella pneumoniae infection.
abstract: Bacterial pneumonia remains an important cause of morbidity and mortality worldwide, especially in immune-compromised patients. Cytokines and chemokines are critical molecules expressed in response to invading pathogens and are necessary for normal lung bacterial host defenses. Here we show that interleukin (IL)-17, a novel cytokine produced largely by CD4+ T cells, is produced in a compartmentalized fashion in the lung after challenge with Klebsiella pneumoniae. Moreover, overexpression of IL-17 in the pulmonary compartment using a recombinant adenovirus encoding murine IL-17 (AdIL-17) resulted in the local induction of tumor necrosis factor-alpha, IL-1beta, macrophage inflammatory protein-2, and granulocyte colony-stimulating factor (G-CSF); augmented polymorphonuclear leukocyte recruitment; and enhanced bacterial clearance and survival after challenge with K. pneumoniae. However, simultaneous treatment with AdIL-17 provided no survival benefit after intranasal K. pneumoniae challenge. These data show that IL-17 may have a role in priming for enhanced chemokine and G-CSF production in the context of lung infection and that optimally timed gene therapy with IL-17 may augment host defense against bacterial pneumonia.
authors.affiliation: Gene Therapy Program, Section of Pulmonary and Critical Care, Louisiana State University Health Sciences Center, New Orleans, Louisiana 70112, USA.
authors.forename: P
authors.initials: P
authors.lastname: Ye
authors.forename: P B
authors.initials: PB
authors.lastname: Garvey
authors.forename: P
authors.initials: P
authors.lastname: Zhang
authors.forename: S
authors.initials: S
authors.lastname: Nelson
authors.forename: G
authors.initials: G
authors.lastname: Bagby
authors.forename: W R
authors.initials: WR
authors.lastname: Summer
authors.forename: P
authors.initials: P
authors.lastname: Schwarzenberger
authors.forename: J E
authors.initials: JE
authors.lastname: Shellito
authors.forename: J K
authors.initials: JK
authors.lastname: Kolls
mesh_terms: D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D054426:Chemokine CXCL2; D018925:Chemokines / Q000378:metabolism; D016179:Granulocyte Colony-Stimulating Factor / Q000378:metabolism; D006801:Humans; D007375:Interleukin-1 / Q000378:metabolism; D020381:Interleukin-17 / Q000235:genetics / Q000378:metabolism*; D007710:Klebsiella Infections / Q000276:immunology*; D007711:Klebsiella pneumoniae / Q000378:metabolism*; D008168:Lung / Q000737:chemistry / Q000276:immunology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018410:Pneumonia, Bacterial / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D054426:Chemokine CXCL2; D018925:Chemokines; C404915:Cxcl2 protein, mouse; D007375:Interleukin-1; D020381:Interleukin-17; D014409:Tumor Necrosis Factor-alpha; D016179:Granulocyte Colony-Stimulating Factor