[Effect of plant glycosides on snail neurons].
The effect of the plant triterpene saponins on Helix pomatia neurons is investigated. It was found that monodesmosidic saponins being perfused to the extracellular solution at concentrations 2[symbol: see text] 10(-3)-10(-4) M induced persistent hyperpolarization of the neurons. By using the patch-clamp technique it was obtained that these substances increased the amplitude of the outward potassium current. At lower concentrations (10(-5)-10(-7) M) saponins either led to the transient reversible oppression of the impulse activity, or didn't change the background activity. The influence of saponins is unspecific and develops during 1-2 min. It was supposed that the effect of triterpene saponins at high concentrations was related with the appearance of nonselective holes in neuron membrane.
Kostiuchenko
O V
OV
Tavrida National V.I. Vernadski University, Simferopol.
Hryshkovets'
V I
VI
Koreniuk
I I
II
D000818:Animals; D002240:Carbohydrate Sequence; D006027:Glycosides / Q000737:chemistry / Q000494:pharmacology*; D006372:Helix, Snails / Q000187:drug effects*; D008969:Molecular Sequence Data; D009474:Neurons / Q000187:drug effects* / Q000502:physiology; D018408:Patch-Clamp Techniques; D010944:Plants / Q000737:chemistry*; D011188:Potassium / Q000378:metabolism; D014315:Triterpenes / Q000737:chemistry / Q000494:pharmacology
D006027:Glycosides; D014315:Triterpenes; D011188:Potassium