Leaf senescence under various gravity conditions: relevance to the dynamics of plant hormones.
Effects of simulated microgravity and hypergravity on the senescence of oat leaf segments excised from the primary leaves of 8-d-old green seedlings were studied using a 3-dimensional (D) clinostat as a simulator of weightlessness and a centrifuge, respectively. During the incubation with water under 1-g conditions at 25 degrees C in the dark, the loss of chlorophyll of the segments was found dramatically immediately after leaf excision, and leaf color completely turned to yellow after 3-d to 4-d incubation. In this case kinetin (10 micromolar) was effective in retarding senescence. The application of simulated microgravity conditions on a 3-D clinostat enhanced chlorophyll loss in the presence or absence of kinetin. The loss of chlorophyll was also enhanced by hypergravity conditions (ca. 8 to 16 g), but the effect was smaller than that of simulated microgravity conditions on the clinostat. Jasmonates (JAs) and abscisic acid (ABA) promoted senescence under simulated microgravity conditions on the clinostat as well as under 1-g conditions. After 2-d incubation with water or 5-d incubation with kinetin, the endogenous levels of JAs and ABA of the segments kept under simulated microgravity conditions on the clinostat remained higher than those kept under 1-g conditions. These findings suggest that physiological processes of leaf senescence and the dynamics of endogenous plant hormone levels are substantially affected by gravity.
Miyamoto
K
K
College of Integrated Arts and Sciences, Osaka Prefecture University, Osaka, Japan.
Yuda
T
T
Shimazu
T
T
Ueda
J
J
D000040:Abscisic Acid / Q000378:metabolism; D000085:Acetates / Q000378:metabolism; D000225:Adenine / Q000031:analogs & derivatives* / Q000494:pharmacology; D018554:Avena / Q000254:growth & development* / Q000378:metabolism; D002498:Centrifugation; D002734:Chlorophyll / Q000378:metabolism; D003517:Cyclopentanes / Q000378:metabolism; D003624:Darkness; D006112:Gravitation; D018471:Hypergravity; D007701:Kinetin; D054883:Oxylipins; D010937:Plant Growth Regulators / Q000378:metabolism* / Q000494:pharmacology; D018515:Plant Leaves / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism*; D012399:Rotation; D018474:Weightlessness Simulation
D000085:Acetates; D003517:Cyclopentanes; D054883:Oxylipins; D010937:Plant Growth Regulators; D002734:Chlorophyll; D000040:Abscisic Acid; C072239:methyl jasmonate; D000225:Adenine; D007701:Kinetin