title: DNA-induced alpha-helical structure in the NH2-terminal domain of histone H1.
abstract: It is important to establish the structural properties of linker histones to understand the role they play in chromatin higher order structure and gene regulation. Here, we use CD, NMR, and IR spectroscopy to study the conformation of the amino-terminal domain of histone H1 degrees, free in solution and bound to the DNA. The NH(2)-terminal domain has little structure in aqueous solution, but it acquires a substantial amount of alpha-helical structure in the presence of trifluoroethanol (TFE). As in other H1 subtypes, the basic residues of the NH(2)-terminal domain of histone H1 degrees are clustered in its COOH-terminal half. According to the NMR results, the helical region comprises the basic cluster (Lys(11)-Lys(20)) and extends until Asp(23). The fractional helicity of this region in 90% TFE is about 50%. His(24) together with Pro(25) constitute the joint between the NH(2)-terminal helix and helix I of the globular domain. Infrared spectroscopy shows that interaction with the DNA induces an amount of alpha-helical structure equivalent to that observed in TFE. As coulombic interactions are involved in complex formation, it is highly likely in the complexes with DNA that the minimal region with alpha-helical structure is that containing the basic cluster. In chromatin, the high positive charge density of the inducible NH(2)-terminal helical element may contribute to the binding stability of the globular domain.
authors.affiliation: Departamento de Bioquimica y Biologia Molecular, Facultad de Ciencias, Universidad Autónoma de Barcelona, 08193 Bellaterra, Barcelona, Spain.
authors.forename: R
authors.initials: R
authors.lastname: Vila
authors.forename: I
authors.initials: I
authors.lastname: Ponte
authors.forename: M
authors.initials: M
authors.lastname: Collado
authors.forename: J L
authors.initials: JL
authors.lastname: Arrondo
authors.forename: M A
authors.initials: MA
authors.lastname: Jiménez
authors.forename: M
authors.initials: M
authors.lastname: Rico
authors.forename: P
authors.initials: P
authors.lastname: Suau
mesh_terms: D000595:Amino Acid Sequence; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry*; D006657:Histones / Q000737:chemistry*; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D011487:Protein Conformation; D013055:Spectrophotometry, Infrared
chemical_list: D006657:Histones; D004247:DNA