Establishment and characterisation of ovine blood monocyte-derived cell lines.
Studies of the important functions in host defense assured by macrophages, both as functional elements and as potential targets for intracellular pathogens, are often inhibited by the lack of a source of large numbers of uniform, well-characterised cells. To address this lack for ovine studies, we have established cell lines from spontaneously-proliferating adherent mononuclear cells from sheep blood. Eight such lines which have been continuously cultured for over 400 passages have phagocytic activities and cytochemical characteristics indicating that they retain the nature of mononuclear phagocytes. They display typical functional membrane proteins such as CD14, Fc receptors and MHC class II. Such cells can facilitate in vitro studies of pathogen-monocyte interactions and can furnish copious amounts of cells for transfer experiments.
Olivier
M
M
Laboratoire de Pathologie Infectieuse et Immunologie, INRA, Centre de Tours, 37380 Nouzilly, France. olivier@tours.inra.fr
Berthon
P
P
Chastang
J
J
Cordier
G
G
Lantier
F
F
D000135:Acid Phosphatase / Q000097:blood; D000818:Animals; D043182:Carboxylesterase; D002265:Carboxylic Ester Hydrolases / Q000097:blood; D018929:Cell Culture Techniques; D002460:Cell Line; D004247:DNA / Q000737:chemistry; D005260:Female; D005802:Genes, MHC Class II / Q000276:immunology; D007150:Immunohistochemistry / Q000662:veterinary; D018950:Lipopolysaccharide Receptors / Q000097:blood; D008264:Macrophages / Q000166:cytology / Q000201:enzymology / Q000276:immunology*; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009000:Monocytes / Q000166:cytology / Q000201:enzymology / Q000276:immunology*; D009195:Peroxidase / Q000097:blood; D012313:RNA / Q000737:chemistry / Q000302:isolation & purification; D011961:Receptors, Fc / Q000097:blood; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D017422:Sequence Analysis, DNA; D012756:Sheep / Q000097:blood / Q000276:immunology*
D018950:Lipopolysaccharide Receptors; D011961:Receptors, Fc; D012313:RNA; D004247:DNA; D009195:Peroxidase; D002265:Carboxylic Ester Hydrolases; D043182:Carboxylesterase; D000135:Acid Phosphatase