A comparative study of three cranial sensory ganglia projecting into the oral cavity: in situ hybridization analyses of neurotrophin receptors and thermosensitive cation channels.
Peripheral cranial sensory nerves projecting into the oral cavity receive food intake stimuli and transmit sensory signals to the central nervous system. To describe and compare the features of the cranial sensory ganglia that innervate the oral cavity, i.e., the trigeminal, petrosal, and geniculate ganglia (TG, PG, and GG, respectively), in situ hybridization was conducted using riboprobes for neurotrophin receptors (TrkA, TrkB, and TrkC), a neurotransmitter (substance P), and ion channels important for thermosensation (VR1 and TREK-1). In TG, all in six probes yielded positive signals to various extent in intensity and frequency. In addition, a strong correlation between the expression of VR1 and those of TrkA and substance P was observed as in the case of the dorsal root ganglia. In PG, positive signals to all six probes were also detected, and the correlation of expression was similar to that shown by TG. On the other hand, most cells in GG were positive to the TrkB probe, and a small number of cells were positive to the TrkC probe, but no significant signal was observed for the other four probes. These results indicate that TG and PG consist of cells that are heterogeneous in terms of neurotrophin requirement and somatosensory functions, and that GG seems to consist mainly of a homogeneous cell type, gustatory neurons. In conclusion, TG, PG, and GG, show gene expression characteristics intrinsic to the three ganglia. It is also concluded that TG and a portion of PG project several types of somatosensory nerves. This is consistent with the finding that GG and a portion of PG project gustatory nerves.
Matsumoto
I
I
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, 113-8657, Tokyo, Japan.
Emori
Y
Y
Ninomiya
Y
Y
Abe
K
K
D000818:Animals; D004435:Eating / Q000502:physiology; D017950:Ganglia, Sensory / Q000033:anatomy & histology* / Q000737:chemistry; D005727:Ganglia, Spinal / Q000033:anatomy & histology / Q000737:chemistry; D020869:Gene Expression Profiling; D005830:Geniculate Ganglion / Q000033:anatomy & histology*; D006358:Hot Temperature; D017403:In Situ Hybridization; D007473:Ion Channels / Q000096:biosynthesis* / Q000235:genetics; D008036:Lingual Nerve / Q000033:anatomy & histology* / Q000737:chemistry; D008297:Male; D008340:Mandibular Nerve / Q000033:anatomy & histology* / Q000737:chemistry; D008442:Maxillary Nerve / Q000033:anatomy & histology* / Q000737:chemistry; D009055:Mouth / Q000294:innervation*; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics; D009474:Neurons / Q000737:chemistry; D015221:Potassium Channels / Q000096:biosynthesis / Q000235:genetics; D024683:Potassium Channels, Tandem Pore Domain; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D020917:Receptor, trkA / Q000096:biosynthesis / Q000235:genetics; D020813:Receptor, trkB / Q000096:biosynthesis / Q000235:genetics; D020812:Receptor, trkC / Q000096:biosynthesis / Q000235:genetics; D011955:Receptors, Drug / Q000096:biosynthesis / Q000235:genetics; D017475:Receptors, Nerve Growth Factor / Q000096:biosynthesis* / Q000235:genetics; D013373:Substance P / Q000096:biosynthesis* / Q000235:genetics; D013649:Taste / Q000502:physiology; D012668:Trigeminal Ganglion / Q000033:anatomy & histology* / Q000737:chemistry
D007473:Ion Channels; D009419:Nerve Tissue Proteins; D015221:Potassium Channels; D024683:Potassium Channels, Tandem Pore Domain; D012333:RNA, Messenger; D011955:Receptors, Drug; D017475:Receptors, Nerve Growth Factor; C103742:potassium channel protein TREK-1; D013373:Substance P; D020917:Receptor, trkA; D020813:Receptor, trkB; D020812:Receptor, trkC