PIRA PCR designer for restriction analysis of single nucleotide polymorphisms.
Primer-introduced restriction analysis (PIRA-PCR) is widely used to detect Single Nucleotide Polymorphisms (SNPs). To create artificial Restriction Fragment Length Polymorphism (RFLP), a mismatch is usually introduced near the end of the primer that is close to the mutation of interest. We describe in this report a www-based computer program that screens for the suitable mismatches, designs the primers, lists the appropriate restriction enzymes and other related information.


BACKGROUND
The computer program, with related descriptions, is available at http://cedar.genetics.soton.ac.uk/public_html/primer2.html.
Ke
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Division of Human Genetics, University of Southampton, Duthie Building, Southampton General Hospital, Southampton SO16 6YD, UK. xke@soton.ac.uk
Collins
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Ye
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D020137:Base Pair Mismatch / Q000235:genetics; D017931:DNA Primers / Q000138:chemical synthesis*; D009154:Mutation; D016133:Polymerase Chain Reaction / Q000379:methods*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D015183:Restriction Mapping / Q000379:methods*; D017422:Sequence Analysis, DNA / Q000379:methods; D012984:Software
D017931:DNA Primers