High-throughput analysis of total nitrogen content that replaces the classic Kjeldahl method.
A high-throughput method for determination of total nitrogen content has been developed. The method involves decomposition of samples, followed by trapping and quantitative colorimetric determination of the resulting ammonia. The present method is rapid, facile, and economical. Thus, it can replace the classic Kjeldahl method through its higher efficiency for determining multiple samples. Compared to the classic method, the present method is economical and environmentally friendly. Based on the present method, a novel reactor was constructed to realize routine high-throughput analyses of multiple samples such as those found for pharmaceutical materials, foods, and/or excrements.
Yasuhara
T
T
Junior College of Tokyo University of Agriculture, Junior College, Sakuragaoka 1-1-1, Setagayaku, Tokyo 156-0054, Japan.
Nokihara
K
K
D000641:Ammonia / Q000032:analysis; D000818:Animals; D002623:Chemistry Techniques, Analytical / Q000295:instrumentation; D003124:Colorimetry / Q000295:instrumentation / Q000379:methods*; D006224:Cricetinae; D005243:Feces / Q000737:chemistry; D005504:Food Analysis / Q000295:instrumentation / Q000379:methods*; D006861:Hydrogen Peroxide / Q000737:chemistry; D009584:Nitrogen / Q000032:analysis*; D012680:Sensitivity and Specificity; D013025:Glycine max / Q000737:chemistry; D013464:Sulfuric Acids / Q000737:chemistry
D013464:Sulfuric Acids; D000641:Ammonia; D006861:Hydrogen Peroxide; D009584:Nitrogen; C033158:sulfuric acid