Measurement of pyruvate in blood by high-performance liquid chromatography with fluorescence detection.
Accurate measurement of pyruvate is particularly important in the detection of mitochondrial enzyme disorders. describe a simple high-performance liquid chromatography assay for pyruvate with derivatization and fluorescence detection. Between-run variation is below 4.5% and the method is linear between I and 1,200 micromol/L. The mean recovery is 101%. After sample pretreatment, the calculated pyruvate concentration proved to be stable for 15 days when samples were kept at -20 degrees C.
Wulkan
R W
RW
Department of Clinical Biochemistry, Medisch Centrum Rijnmond-Zuid, Rotterdam, The Netherlands. r.wulkan@zuiderziekenhuis.nl
Verwers
R
R
Neele
M
M
Mantel
M J
MJ
D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism*; D005615:Freezing; D006801:Humans; D016014:Linear Models; D010655:Phenylenediamines / Q000737:chemistry / Q000378:metabolism; D019289:Pyruvic Acid / Q000097:blood*; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013048:Specimen Handling; D013997:Time Factors
D005456:Fluorescent Dyes; D010655:Phenylenediamines; D019289:Pyruvic Acid; C034193:1,2-diaminobenzene