title: Complex between Peptostreptococcus magnus protein L and a human antibody reveals structural convergence in the interaction modes of Fab binding proteins.
abstract: BACKGROUND
  Peptostreptococcus magnus protein L (PpL) is a multidomain, bacterial surface protein whose presence correlates with virulence. It consists of up to five homologous immunoglobulin binding domains that interact with the variable (VL) regions of kappa light chains found on two thirds of mammalian antibodies.
  
  
  RESULTS
  We refined the crystal structure of the complex between a human antibody Fab fragment (2A2) and a single PpL domain (61 residues) to 2.7 A. The asymmetric unit contains two Fab molecules sandwiching a single PpL domain, which contacts similar VL framework regions of two light chains via independent interfaces. The residues contacted on VL are remote from the hypervariable loops. One PpL-Vkappa interface agrees with previous biochemical data, while the second is novel. Site-directed mutagenesis and analytical-centrifugation studies suggest that the two PpL binding sites have markedly different affinities for VL. The PpL residues in both interactions are well conserved among different Peptostreptococcus magnus strains. The Fab contact positions identified in the complex explain the high specificity of PpL for antibodies with kappa rather than lambda chains.
  
  
  CONCLUSIONS
  The PpL-Fab complex shows the first interaction of a bacterial virulence factor with a Fab light chain outside the conventional combining site. Structural comparison with two other bacterial proteins interacting with the Fab heavy chain shows that PpL, structurally homologous to streptococcal SpG domains, shares with the latter a similar binding mode. These two bacterial surface proteins interact with their respective immunoglobulin regions through a similar beta zipper interaction.
authors.affiliation: Département d'Ingénierie et d'Etudes des Protéines, Commissariat à l'Energie Atomique, Centre d'Etudes Saclay, F-91191, Gif-sur-Yvette, France.
authors.forename: M
authors.initials: M
authors.lastname: Graille
authors.forename: E A
authors.initials: EA
authors.lastname: Stura
authors.forename: N G
authors.initials: NG
authors.lastname: Housden
authors.forename: J A
authors.initials: JA
authors.lastname: Beckingham
authors.forename: S P
authors.initials: SP
authors.lastname: Bottomley
authors.forename: D
authors.initials: D
authors.lastname: Beale
authors.forename: M J
authors.initials: MJ
authors.lastname: Taussig
authors.forename: B J
authors.initials: BJ
authors.lastname: Sutton
authors.forename: M G
authors.initials: MG
authors.lastname: Gore
authors.forename: J B
authors.initials: JB
authors.lastname: Charbonnier
mesh_terms: D000595:Amino Acid Sequence; D000936:Antigen-Antibody Complex; D001426:Bacterial Proteins; D018360:Crystallography, X-Ray; D004268:DNA-Binding Proteins / Q000737:chemistry*; D006801:Humans; D006860:Hydrogen Bonding; D007140:Immunoglobulin Fab Fragments / Q000737:chemistry*; D007075:Immunoglobulin M / Q000737:chemistry; D007136:Immunoglobulins / Q000737:chemistry; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D010462:Peptostreptococcus / Q000737:chemistry*; D011485:Protein Binding; D017434:Protein Structure, Tertiary
chemical_list: D000936:Antigen-Antibody Complex; D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D007140:Immunoglobulin Fab Fragments; D007075:Immunoglobulin M; D007136:Immunoglobulins; C116593:L-protein, Peptococcus magnus