[Effect of destruction of Escherichia coli cells on the catalytic ability of catalase].
The possibility for investigation of catalase (CAT) activity under the conditions of intact E. coli cells was estimated. This approach is based on the possibility of hydrogen peroxide freely cross biological membranes. CAT activity of native cells had a broad maximum between pH values 4.5 and 7.5. Desintegration of cells by freezing--thawing and ultrasonication indicated that there were two CAT activity peaks at pH values about 3.5 and 7.0. Activity of CAT with acid pH-optimum decreased at cell desintegration, but one with neutral pH-optimum was rather stable under this procedure. The enzyme in native conditions was less sensitive to the inhibition by high concentrations of hydrogen peroxide than its counterpart from destroyed cells. Activity of CAT in native and desintegrated cell preparations had different sensitivity to heating and inhibition by reduced glutathione, but it was inhibited by azide similarly. Difference in the CAT properties of native and desintegrated bacteria preparations may be explained by different possibility to penetrate cell membrane by reagents and/or by possible modification of the enzyme properties at destruction of native microenvironment.
Semchyshin
H M
HM
Stephanic Pricarpathian University, Ivano-Frankivsk State Medical Academy, Ukraine. lushchak@pu.if.ua
Dyl'ovyĭ
M V
MV
Klymenko
A O
AO
Lushchak
V I
VI
D002374:Catalase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D002384:Catalysis; D004926:Escherichia coli / Q000201:enzymology*; D005615:Freezing; D005978:Glutathione / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D013379:Substrate Specificity
D006861:Hydrogen Peroxide; D002374:Catalase; D005978:Glutathione