title: Sequence polymorphism of the Salmonella plasmid virulence factor D (SpvD) in Salmonella enterica isolates of animal origin.
abstract: The nucleotide sequence encoding the Salmonella plasmid virulence factor D (SpvD) was determined in 17 Salmonella strains that were different in O and H antigen patterns, animal host and geographical origin, and year of isolation. Nucleotide sequence comparison revealed the existence of at least nine spvD alleles resulting in 8 SpvD protein variants although the nucleotide sequences were highly similar (identity 98.8-100%). The spvD gene products differed from each other in up to 4 amino acid residues only with the exception of the carboxy-terminally truncated SpvD variant of one S. Gallinarum field isolate. The highly conserved primary structure of SpvD in epidemiologically relevant salmonellae suggests that this virulence factor is a promising antigen candidate for diagnostic purposes (i.e. antibody detection in infected animals) but also for immunoprophylaxis in farm animal species.
authors.affiliation: Institute for Hygiene and Infectious Diseases of Animals, Justus Liebig University Giessen, Germany. rolf.bauerfeind@vetmed.uni-giessen.de
authors.forename: R
authors.initials: R
authors.lastname: Bauerfeind
authors.forename: S
authors.initials: S
authors.lastname: Barth
authors.forename: R
authors.initials: R
authors.lastname: Weiss
authors.forename: G
authors.initials: G
authors.lastname: Baljer
mesh_terms: D036002:ADP Ribose Transferases / Q000737:chemistry / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D001483:Base Sequence; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis / Q000737:chemistry; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D010802:Phylogeny; D010957:Plasmids; D011110:Polymorphism, Genetic; D012475:Salmonella / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity; D012481:Salmonella Infections, Animal / Q000235:genetics*; D014774:Virulence; D037521:Virulence Factors
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D017931:DNA Primers; D004269:DNA, Bacterial; C445368:SpvD protein, Salmonella enterica; D037521:Virulence Factors; D036002:ADP Ribose Transferases