title: Enhancement of stromal cell-derived factor-1alpha-induced chemotaxis for CD4/8 double-positive thymocytes by fibronectin and laminin in mice.
abstract: Stromal cell-derived factor-1alpha (SDF-1alpha) is a chemokine abundantly expressed in the thymus. However, a potential role of SDF-1alpha in the thymus has been under consideration, since no appreciable difference was detected in the migratory responsiveness to the SDF-1alpha between cortical and medullary thymocytes. In the present study, we examined the effects of extracellular matrix (ECM) on the responsiveness of murine thymocytes to several chemokines including SDF-1alpha. In the absence of ECM, chemotactic activity of SDF-1alpha for cortical (CD4/8 double-positive) thymocytes was almost same as that for medullary (CD4 or CD8 single-positive) thymocytes. In contrast, the chemotactic activity of SDF-1alpha for cortical thymocytes was considerably (more than 10-fold) enhanced by laminin or fibronectin as compared with that for medullary thymocytes. Chemotactic activities of macrophage-derived chemokine and macrophage inflammatory protein-3beta for both cortical and medullary thymocytes were only slightly enhanced by fibronectin or laminin. Thus, fibronectin and laminin appear to enhance the chemotactic activity of SDF-1alpha for cortical thymocytes selectively. Addition of a monoclonal antibody against CD29 showed no inhibitory effect on the enhanced chemotactic activity of SDF-1alpha, suggesting that the other unknown receptor(s) is involved in this enhancement. Our present data demonstrate that SDF-1alpha in the presence of fibronectin or laminin is involved in the distribution of developing thymocytes.
authors.affiliation: Division of Immunobiology, Institute for Genetic Medicine, Hokkaido University, Sapporo, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Yanagawa
authors.forename: K
authors.initials: K
authors.lastname: Iwabuchi
authors.forename: K
authors.initials: K
authors.lastname: Onoé
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000276:immunology / Q000378:metabolism; D000945:Antigens, Differentiation, T-Lymphocyte / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D054415:Chemokine CCL19; D054422:Chemokine CCL22; D054377:Chemokine CXCL12; D019742:Chemokines, CC / Q000276:immunology; D019743:Chemokines, CXC / Q000276:immunology*; D002634:Chemotaxis, Leukocyte / Q000276:immunology*; D005109:Extracellular Matrix / Q000276:immunology; D005260:Female; D005353:Fibronectins / Q000276:immunology; D019012:Integrin beta1 / Q000276:immunology; D039663:Integrin beta4; D007797:Laminin / Q000276:immunology; D037181:Lectins, C-Type; D051379:Mice; D008810:Mice, Inbred C57BL; D017154:Stromal Cells / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology; D010566:Virulence Factors, Bordetella / Q000276:immunology; D000099192:CD69 Antigens
chemical_list: D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D054415:Chemokine CCL19; D054422:Chemokine CCL22; D054377:Chemokine CXCL12; D019742:Chemokines, CC; D019743:Chemokines, CXC; D005353:Fibronectins; D019012:Integrin beta1; D039663:Integrin beta4; D007797:Laminin; D037181:Lectins, C-Type; D010566:Virulence Factors, Bordetella; D000099192:CD69 Antigens; C515461:Ccl19 protein, mouse; C515483:Ccl22 protein, mouse; C515256:Cxcl12 protein, mouse