title: Sd(a)-antigen-like structures carried on core 3 are prominent features of glycans from the mucin of normal human descending colon.
abstract: This paper describes structural characterization by NMR, MS and degradative studies of mucin glycans from normal human descending colon obtained freshly at autopsy. The saccharides were mainly based on core 3 (GlcNAcbeta1-3GalNAc). Among the terminal saccharide determinants Sd(a)/Cad-antigen-like structures were prominent, and Lewis x, sialyl Lewis x and sulphated Lewis x were found as minor components, whereas blood group H and A antigenic determinants were absent. The saccharides were markedly different from those of mucins from colon cancers or colon cancer cell lines analysed so far, in which cores 1 and 2 are prominent features, and in which various other terminal determinants have been found, but not Sd(a)/Cad.
authors.affiliation: Laboratoire de Glycobiologie structurale et fonctionnelle, Unité Mixte du Centre National de la Recherche Scientifique, Université des Sciences et Technologies de Lille, Villeneuve d'Ascq Cedex, France.
authors.forename: C
authors.initials: C
authors.lastname: Capon
authors.forename: E
authors.initials: E
authors.lastname: Maes
authors.forename: J C
authors.initials: JC
authors.lastname: Michalski
authors.forename: H
authors.initials: H
authors.lastname: Leffler
authors.forename: Y S
authors.initials: YS
authors.lastname: Kim
mesh_terms: D001344:Autopsy; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D003106:Colon / Q000737:chemistry*; D006649:Histocompatibility Antigens / Q000737:chemistry; D006801:Humans; D007413:Intestinal Mucosa / Q000737:chemistry*; D016256:Lewis X Antigen / Q000737:chemistry / Q000031:analogs & derivatives; D009077:Mucins / Q000737:chemistry* / Q000302:isolation & purification; D019906:Nuclear Magnetic Resonance, Biomolecular; D009844:Oligosaccharides / Q000737:chemistry* / Q000302:isolation & purification; D011134:Polysaccharides / Q000737:chemistry; D000080506:Sialyl Lewis X Antigen; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: C402829:3-sulfated Lewis x; D006649:Histocompatibility Antigens; D016256:Lewis X Antigen; D009077:Mucins; D009844:Oligosaccharides; D011134:Polysaccharides; C106678:Sd(a) determinant; D000080506:Sialyl Lewis X Antigen