title: A simple method for the assay of colistin in human plasma, using pre-column derivatization with 9-fluorenylmethyl chloroformate in solid-phase extraction cartridges and reversed-phase high-performance liquid chromatography.
abstract: A simple, selective and sensitive high-performance liquid chromatographic (HPLC) method is described for the determination of colistin in human plasma. Derivatization with 9-fluorenylmethyl chloroformate was performed in the same solid-phase extraction C18 cartridge used for sample pre-treatment, followed by reversed-phase HPLC with fluorimetric detection. Quantification was achieved using the ratio of the summed peak areas of colistin A and B derivatives to that of the derivative of netilmicin (internal standard). Linear calibration curves were obtained within the concentrations of colistin sulfate from 0.10 to 4.0 mg/l in plasma. Accuracy was within 10% and reproducibility (RSD) was less than 10%.
authors.affiliation: Centre for Pharmaceutical Research, University of South Australia, Adelaide Australia.
authors.forename: J
authors.initials: J
authors.lastname: Li
authors.forename: R W
authors.initials: RW
authors.lastname: Milne
authors.forename: R L
authors.initials: RL
authors.lastname: Nation
authors.forename: J D
authors.initials: JD
authors.lastname: Turnidge
authors.forename: K
authors.initials: K
authors.lastname: Coulthard
authors.forename: D W
authors.initials: DW
authors.lastname: Johnson
mesh_terms: D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003091:Colistin / Q000097:blood*; D005449:Fluorenes / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D013058:Mass Spectrometry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D005449:Fluorenes; D005456:Fluorescent Dyes; C054007:1-(9-fluorenyl)methyl chloroformate; D003091:Colistin