title: New aspects of the glucose activation of the H(+)-ATPase in the yeast Saccharomyces cerevisiae.
abstract: The glucose-induced activation of plasma membrane ATPase from Saccharomyces cerevisiae was first described by Serrano in 1983. Many aspects of this signal transduction pathway are still obscure. In this paper, evidence is presented for the involvement of Snf3p as the glucose sensor related to this activation process. It is shown that, in addition to glucose detection by Snf3p, sugar transport is also necessary for activation of the ATPase. The participation of the G protein, Gpa2p, in transducing the internal signal (phosphorylated sugars) is also demonstrated. Moreover, the involvement of protein kinase C in the regulation of ATPase activity is confirmed. Finally, a model pathway is presented for sensing and transmission of the glucose activation signal of the yeast H(+)-ATPase.
authors.affiliation: Laboratório de Bioquı́mica e Fisiologia de Microrganismos, Núcleo de Pesquisas em Ciências Biológicas, Escola de Farmácia, Universidade Federal de Ouro Preto, Campus do Morro do Cruzeiro - 35.400-000 Ouro Preto, MG, Brazil1.
authors.forename: M A A
authors.initials: MAA
authors.lastname: Souza
authors.affiliation: Laboratório de Bioquı́mica e Fisiologia de Microrganismos, Núcleo de Pesquisas em Ciências Biológicas, Escola de Farmácia, Universidade Federal de Ouro Preto, Campus do Morro do Cruzeiro - 35.400-000 Ouro Preto, MG, Brazil1.
authors.forename: M J
authors.initials: MJ
authors.lastname: Trópia
authors.affiliation: Laboratório de Bioquı́mica e Fisiologia de Microrganismos, Núcleo de Pesquisas em Ciências Biológicas, Escola de Farmácia, Universidade Federal de Ouro Preto, Campus do Morro do Cruzeiro - 35.400-000 Ouro Preto, MG, Brazil1.
authors.forename: R L
authors.initials: RL
authors.lastname: Brandão
mesh_terms: D003470:Culture Media; D004789:Enzyme Activation; D005656:Fungal Proteins / Q000378:metabolism; D044385:GTP-Binding Protein alpha Subunits; D015966:Gene Expression Regulation, Fungal; D005947:Glucose / Q000378:metabolism*; D020962:Heterotrimeric GTP-Binding Proteins / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D009004:Monosaccharide Transport Proteins / Q000378:metabolism; D011493:Protein Kinase C / Q000378:metabolism; D006180:Proton-Translocating ATPases / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000254:growth & development; D029701:Saccharomyces cerevisiae Proteins; D015398:Signal Transduction
chemical_list: D003470:Culture Media; D005656:Fungal Proteins; D044385:GTP-Binding Protein alpha Subunits; D008565:Membrane Proteins; D009004:Monosaccharide Transport Proteins; C104463:SNF3 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D011493:Protein Kinase C; D020928:Mitogen-Activated Protein Kinases; D006180:Proton-Translocating ATPases; C083907:Gpa2 protein, S cerevisiae; D020962:Heterotrimeric GTP-Binding Proteins; D005947:Glucose