title: Tumor necrosis factor-alpha/interleukin-10 balance in normal and cystic fibrosis children.
abstract: BACKGROUND
  The balance between tumor necrosis factor-alpha (TNF-alpha) and interleukin-10 (IL-10) is important for immune homeostasis maintenance. Exuberant production of TNF-alpha contributes to overwhelming inflammatory response and tissue damage. But, commonly, increase in TNF-alpha is counterbalanced by simultaneous synthesis of an anti-inflammatory cytokine IL-10, which suppresses production of many activating and regulatory mediators.
  
  
  OBJECTIVE
  In the present study, the relationships between TNF-alpha and IL-10 in the plasma of healthy school-children and cystic fibrosis (CF) patients have been investigated.
  
  
  METHODS
  Blood samples were obtained from 12 CF patients with chronic pulmonary disease and 18 healthy schoolchildren vaccinated with live attenuated rubella vaccine. IL-10 and TNF-alpha were determined in the plasma samples using commercially available enzyme-linked immunosorbent assay kits.
  
  
  RESULTS
  Before vaccination, most healthy children (13 of 18) demonstrated superiority of pro-inflammatory TNF-alpha over anti-inflammatory IL-10 (TNF-alpha/IL-10 > 1). In these subjects, a significant positive linear association between the cytokine values has been found. Vaccine challenge resulted in a marked reduction of TNF-alpha/IL-10 ratios. In addition, a disappearance of correlation between the cytokine values was observed. Such disturbance was related to exuberant elevation of the IL-10 levels after inoculation. On the contrary, in CF individuals, plasma cytokine values remained in strong linear association independently of TNF-alpha or IL-10 predominance. No spikes in the plasma levels of IL-10 in CF patients during a 6-month observation period have been revealed.
  
  
  CONCLUSIONS
  There were no fundamental differences between CF and healthy children in the regulation of TNF-alpha and IL-10 secretion. Thus, immune quiescence seemed to be associated with the predominance of TNF-alpha, whereas immune disturbance was characterized by IL-10 superiority. The only abnormality that was found in CF patients consisted of their inability to produce unlimitedly IL-10 in response to antigen stimuli.
authors.affiliation: Laboratory of Immunogenetics, Research Centre for Medical Genetics, Moscow, Russia.
authors.forename: G V
authors.initials: GV
authors.lastname: Shmarina
authors.forename: A L
authors.initials: AL
authors.lastname: Pukhalsky
authors.forename: S N
authors.initials: SN
authors.lastname: Kokarovtseva
authors.forename: D A
authors.initials: DA
authors.lastname: Pukhalskaya
authors.forename: L A
authors.initials: LA
authors.lastname: Shabalova
authors.forename: N I
authors.initials: NI
authors.lastname: Kapranov
authors.forename: N J
authors.initials: NJ
authors.lastname: Kashirskaja
mesh_terms: D000293:Adolescent; D002648:Child; D003550:Cystic Fibrosis / Q000276:immunology*; D006801:Humans; D016753:Interleukin-10 / Q000097:blood*; D013223:Statistics as Topic; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*; D014611:Vaccination
chemical_list: D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10