[Preparation of bacteria--degraders of alkylnaphthalenesulfonate by a molecular breeding method].
Transconjugant strain of bacteria degraders of alkylnaphthalenesulphonate(ANS)--P Pseudomonas alcaligenes TR (NPL-41) was obtained under conditions of ANS selective pressure and flow cultivation of bacteria destructors of alkylbenzene sulphonate (ABS) P. alcaligenes TR (pABS) and naphthalene P. putida BS438 (NPL-41) and their immobilization on an inert carrier. Transconjugant P. alcaligenes TR(NPL-41) was prepared by means of conjugative transfer of plasmid NPL-41 from P. putida BS438 to P. alcaligenes TR. The check out of transconjugant under conditions of flow cultivation showed their ability to degradation of ANS. Investigation of the stability of plasmid NPL-41 in the transconjugant P. alcaligenes TR(NPL-41), obtained by molecular breeding under conditions of flow cultivation and by conjugative transfer, showed the transconjugant prepared by the former method to be more stable.
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Ovcharenko Institute of Biocolloid Chemistry, NAS of Ukraine, Kyiv. s@dybkov.kiev.ua
D001483:Base Sequence; D001673:Biodegradation, Environmental; D003227:Conjugation, Genetic; D017931:DNA Primers; D009282:Naphthalenesulfonates / Q000378:metabolism*; D011549:Pseudomonas / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D013045:Species Specificity
D017931:DNA Primers; D009282:Naphthalenesulfonates