title: Separation and determination of protocatechuic aldehyde and protocatechuic acid in Salivia miltorrhrza by capillary electrophoresis with amperometric detection.
abstract: Capillary electrophoresis with amperometric detection was applied to separate and determine protocatechuic aldehyde and protocatechuic acid in Salivia miltorrhrza preparations. The electrode used was a 0.3 mm diameter carbon disk electrode fixed in a wall-jet with amperometric detection. Under the optimum conditions, the two analytes were separated completely within 8 min. Excellent linearity was obtained in the concentration ranges of 0.25-100.0 microg ml(-1) and 0.50-100.0 microg ml(-1) for protocatechuic aldehyde and protocatechuic acid, respectively. The detection limits were 0.10 microg ml(-1) of protocatechuic aldehyde and 0.25 microg ml(-1) of protocatechuic acid, which were found to be lower than those of other methods that determine protocatechuic aldehyde (3,4-dihydroxybenzaldehyde) and protocatechuic acid (3,4-dihydrobenzoic acid) simultaneously. The mean recoveries of protocatechuic aldehyde and protocatechuic acid were 97.4% and 103.3%. This method has been successfully applied to monitor these two components in real samples such as Salivia miltorrhrza and its two traditional Chinese medicinal preparations.
authors.affiliation: Department of Chemistry, Fuzhou University, Fujian, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Pan
authors.forename: L
authors.initials: L
authors.lastname: Zhang
authors.forename: G
authors.initials: G
authors.lastname: Chen
mesh_terms: D016588:Anticarcinogenic Agents / Q000032:analysis*; D000925:Anticoagulants / Q000032:analysis*; D001547:Benzaldehydes / Q000032:analysis*; D002396:Catechols / Q000032:analysis*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods; D006801:Humans; D062385:Hydroxybenzoates / Q000032:analysis*
chemical_list: D016588:Anticarcinogenic Agents; D000925:Anticoagulants; D001547:Benzaldehydes; D002396:Catechols; D004365:Drugs, Chinese Herbal; D062385:Hydroxybenzoates; C009091:protocatechuic acid; C005581:protocatechualdehyde