title: Biochemical effects of dietary intake of different broccoli samples. II. Multivariate analysis of contributions of specific glucosinolates in modulating cytochrome P-450 and antioxidant defense enzyme activities.
abstract: Dietary broccoli exposure modulates various cytochrome P-450 (CYP)-associated activities and antioxidant defense enzyme activities in liver, colon, and kidney of rats. We present an analysis by the partial least-square method (PLS) of the contribution of single glucosinolates in modulating xenobiotic metabolizing and antioxidant defense enzyme activities. Generally, modulation of colonic enzyme activities was well described (58% to 75%) by models consisting of 3 principal components (PCs). The indolyl glucosinolates were not the only major contributors to the regulation of colonic 7-ethoxyresorufin O-deethylase (EROD) and 7-methoxyresorufin O-demethylase (MROD) activities, as would be expected from results of previous experiments testing the pure compounds, glucobrassicin (GB), neoglucobrassicin (NeoGB), and 4-methoxyglucobrassicin (4-MeOGB). In hepatic and renal microsomes, the modulation of enzyme activities could be partly described for hepatic and renal 7-pentoxyresorufin O-deethylase (PROD) activities (42% to 44%, 3 to 4 PCs), hepatic superoxide dismutase activity (45%, 2 PCs), and renal glutathione peroxidase (GSH Px) and glutathione reductase (GSSG Red) activities (43%, 3 PCs). These results indicate that substances other than glucosinolates in the complex mixtures modulate hepatic EROD, MROD, GSH Px, and GSSG Red activities or that the active glucosinolate metabolites vary in their systemic disposition.
authors.affiliation: Department of Life Sciences and Chemistry, Roskilde University, Roskilde, Denmark.
authors.forename: O
authors.initials: O
authors.lastname: Vang
authors.forename: J
authors.initials: J
authors.lastname: Mortensen
authors.forename: O
authors.initials: O
authors.lastname: Andersen
mesh_terms: D000818:Animals; D001937:Brassica / Q000737:chemistry / Q000378:metabolism*; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D004032:Diet; D004789:Enzyme Activation; D005961:Glucosinolates / Q000008:administration & dosage* / Q000032:analysis / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D015394:Molecular Structure; D015999:Multivariate Analysis; D010088:Oxidoreductases / Q000378:metabolism*; D051381:Rats; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D005961:Glucosinolates; D003577:Cytochrome P-450 Enzyme System; D010088:Oxidoreductases; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase