title: Flow cytometric and histologic evaluation in a large cohort of patients with ulcerative colitis: correlation with clinical characteristics and impact on surveillance.
abstract: OBJECTIVE
  To examine the prevalence of DNA aneuploidy as a function of the extent of ulcerative colitis and to study the correlation of aneuploidy with clinical characteristics. Furthermore, the occurrence of aneuploidy and dysplasia during colonoscopic surveillance was studied in a subset of these patients.
  
  
  METHODS
  By analyzing 5404 biopsy samples of 368 patients with ulcerative colitis, we have evaluated the importance of DNA ploidy measured by flow cytometry. We have also investigated the influence of extent (219 patients with extensive or total colitis vs. 149 patients with localized colitis) and duration of colitis on the development of dysplasia (patients with biopsy specimens that showed inflammation alone were compared with those with biopsy specimens that were equivocal or positive for dysplasia) and aneuploidy. Included was a subgroup of patients with ulcerative colitis and primary sclerosing cholangitis (n = 16).
  
  
  RESULTS
  Aneuploidy was found in 8.7 percent (32/368) of all patients. The prevalence of aneuploidy increased by the extent of ulcerative colitis (2 percent localized, 6.8 percent extensive colitis, 14.9 percent total colitis). The frequency of aneuploidy was higher in patients with disease duration longer than 10 years (P = 0.007). Patients with ulcerative colitis and primary sclerosing cholangitis were more likely to develop aneuploidy (9/16, 56.3 percent vs. 14/120, 11.7 percent; P < 0.001) and dysplasia (4/16, 25 percent vs. 10/120, 8.3 percent; P = 0.06) than patients without primary sclerosing cholangitis.
  
  
  CONCLUSIONS
  Because DNA aneuploidy represents an early alteration during neoplastic transformation in ulcerative colitis, flow cytometry is a valuable tool in the surveillance of those patients. Primary sclerosing cholangitis represents an additional risk factor for the development of DNA aneuploidy and dysplasia.
authors.affiliation: Department of Internal Medicine I, Eberhard-Karls-University Tübingen, Tübingen, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Holzmann
authors.forename: B
authors.initials: B
authors.lastname: Klump
authors.forename: F
authors.initials: F
authors.lastname: Borchard
authors.forename: M
authors.initials: M
authors.lastname: Gregor
authors.forename: R
authors.initials: R
authors.lastname: Porschen
mesh_terms: D000782:Aneuploidy; D001706:Biopsy; D002453:Cell Cycle; D015209:Cholangitis, Sclerosing / Q000150:complications; D015331:Cohort Studies; D003093:Colitis, Ulcerative / Q000150:complications / Q000235:genetics / Q000473:pathology*; D003113:Colonoscopy; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D011003:Ploidies; D011230:Precancerous Conditions / Q000473:pathology*; D012307:Risk Factors