Respiratory activity of isolated rat hepatocytes following cold storage and subsequent rewarming: a comparison of sucrose-based and University of Wisconsin solutions.
Investigations were carried out on the respiratory function of isolated rat hepatocytes after cold storage alone for periods up to 48 h in either sucrose-based solution (SBS) or University of Wisconsin (UW) solution and after subsequent normothermic preincubation. In both SBS and UW, cold storage for 24 h depressed respiratory function (to 21 +/- 3 and 23 +/- 3 nmol O(2)/min/10(6) cells, respectively) compared to control cell values (31 +/- 3 and 33 +/- 5 nmol O(2)/min/10(6) cells; P < 0.01 in each case). However, normothermic preincubation for 60 min returned respiratory activity to control values (for SBS and UW storage: 41 +/- 6 and 40 +/- 5 nmol O(2)/min/10(6) cells; for control cells: 43 +/- 5 and 46 +/- 6 nmol O(2)/min/10(6) cells). Storage for 48 h in both SBS and UW allowed further depression of respiratory activity, with no recovery after preincubation. Stimulation of respiration by succinate in hepatocytes stored for longer periods was suggestive of increased membrane permeability. Both SBS and UW are effective storage solutions for isolated hepatocytes for up to 24 h as judged by aerobic metabolism, but significant damage was expressed in both solutions when preservation was extended.
Kravchenko
L P
LP
Institute for Problems of Cryobiology and Cryomedicine, National Academy of Sciences of the Ukraine, 23 Pereyaslavskaya Str., Kharkov, 61015, Ukraine.
Petrenko
A Y
AY
Somov
A Y
AY
Grischenko
V I
VI
Fuller
B J
BJ
D000241:Adenosine; D000493:Allopurinol; D000818:Animals; D002463:Cell Membrane Permeability; D019069:Cell Respiration; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents; D005978:Glutathione; D022781:Hepatocytes / Q000378:metabolism*; D066298:In Vitro Techniques; D007328:Insulin; D007700:Kinetics; D019188:Organ Preservation Solutions; D011887:Raffinose; D051381:Rats; D013395:Sucrose
D003451:Cryoprotective Agents; D007328:Insulin; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D013395:Sucrose; D000493:Allopurinol; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose