Role of beta-dystrobrevin in nonmuscle dystrophin-associated protein complex-like complexes in kidney and liver.
beta-Dystrobrevin is a dystrophin-related and -associated protein that is highly expressed in brain, kidney, and liver. Recent studies with the kidneys of the mdx3Cv mouse, which lacks all dystrophin isoforms, suggest that beta-dystrobrevin, and not the dystrophin isoforms, may be the key component in the assembly of complexes similar to the muscle dystrophin-associated protein complexes (DPC) in nonmuscle tissues. To understand the role of beta-dystrobrevin in the function of nonmuscle tissues, we generated beta-dystrobrevin-deficient (dtnb(-/-)) mice by gene targeting. dtnb(-/-) mice are healthy, fertile, and normal in appearance. No beta-dystrobrevin was detected in these mice by Western blotting or immunocytochemistry. In addition, the levels of several beta-dystrobrevin-interacting proteins, namely Dp71 isoforms and the syntrophins, were greatly reduced from the basal membranes of kidney tubules and liver sinusoids and on Western blots of crude kidney and liver microsomes of beta-dystrobrevin-deficient mice. However, no abnormality was detected in the ultrastructure of membranes of kidney and liver cells or in the renal function of these mice. beta-Dystrobrevin may therefore be an anchor or scaffold for Dp71 and syntrophin isoforms, as well as other associating proteins at the basal membranes of kidney and liver, but is not necessary for the normal function of these mice.
Loh
N Y
NY
Department of Human Anatomy and Genetics, University of Oxford, Oxford OX1 3QX, United Kingdom.
Nebenius-Oosthuizen
D
D
Blake
D J
DJ
Smith
A J
AJ
Davies
K E
KE
D000483:Alleles; D000818:Animals; D015153:Blotting, Western; D016189:Dystrophin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D049029:Dystrophin-Associated Proteins; D005260:Female; D005838:Genotype; D006801:Humans; D007150:Immunohistochemistry; D007668:Kidney / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008297:Male; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D051379:Mice; D008822:Mice, Transgenic; D008856:Microscopy, Fluorescence; D008862:Microsomes, Liver / Q000378:metabolism; D008957:Models, Genetic; D009124:Muscle Proteins / Q000737:chemistry / Q000378:metabolism; D009154:Mutation; D010641:Phenotype; D011485:Protein Binding; D020033:Protein Isoforms; D012737:Sex Factors; D014018:Tissue Distribution
D016189:Dystrophin; D049029:Dystrophin-Associated Proteins; D008565:Membrane Proteins; D009124:Muscle Proteins; D020033:Protein Isoforms; C085339:apo-dystrophin 1; C098885:dystrobrevin; C091824:syntrophin