title: Application of analytical and preparative high-speed counter-current chromatography for separation of lycopene from crude extract of tomato paste.
abstract: Lycopene was isolated from 100 mg of crude extract of tomato paste containing about 9% of lycopene. Analytical high-speed counter-current chromatography (HSCCC) was first used for the systematic selection of the two-phase solvent system. Then preparative HSCCC separation was performed with a nonaqueous solvent system composed of n-hexane-dichloromethane-acetonitrile at an optimum volume ratio of 10:3.5:6.5. This yielded 8.6 mg of lycopene at over 98.5% purity as determined by HPLC analysis.
authors.affiliation: Beijing Research Center for Separation and Purification Technologies of Natural Prodiucts, Beijing Institute of New Technology Application, PR China.
authors.forename: Y
authors.initials: Y
authors.lastname: Wei
authors.forename: T
authors.initials: T
authors.lastname: Zhang
authors.forename: G
authors.initials: G
authors.lastname: Xu
authors.forename: Y
authors.initials: Y
authors.lastname: Ito
mesh_terms: D002338:Carotenoids / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D003377:Countercurrent Distribution / Q000379:methods*; D000077276:Lycopene; D018551:Solanum lycopersicum / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D010936:Plant Extracts / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D010936:Plant Extracts; D002338:Carotenoids; D000077276:Lycopene