Detection of protein-protein interactions in vivo based on protein splicing.
In mammalian cells, protein-protein interactions constitute essential regulatory steps that modulate the activity of signaling pathways. In recent years, several approaches towards understanding the interactions have been developed. We describe herein a new method for detecting protein-protein interactions in vivo based on protein splicing and highlight some potential applications of this technique.
Ozawa
T
T
Department of Chemistry, School of Science, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
Umezawa
Y
Y
D000818:Animals; D004706:Endodeoxyribonucleases / Q000378:metabolism; D049452:Green Fluorescent Proteins; D006801:Humans; D008156:Luciferases / Q000737:chemistry; D008164:Luminescent Proteins / Q000378:metabolism; D055095:Optics and Photonics; D011485:Protein Binding / Q000502:physiology*; D019154:Protein Splicing; D011993:Recombinant Fusion Proteins / Q000737:chemistry
D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; D008156:Luciferases; D004706:Endodeoxyribonucleases