title: Measurement of asialylated LDL in the blood of patients with coronary artery disease by antibody-lectin sandwich assay.
abstract: Elevated concentrations of asialylated LDL (asialo-LDL) have been reported in patients with coronary artery disease (CAD). This may stimulate lipid accumulation in arterial intima cells and promote atherosclerosis. To investigate asialo-LDL as a potential risk-factor for coronary atherogenesis, we developed an antibody-lectin sandwich assay to measure levels in serum from CAD patients and age-matched control subjects. LDL was captured with an anti-apolipoprotein (apo) B antibody and asialylated oligosaccharides measured using the biotinylated D-galactose (D-gal) binding lectin, Ricinus communis agglutinin 120 (RCA120), and a streptavidin-alkaline phosphatase conjugate. For the control and atherosclerotic subjects, median [interquartile range (IQR)] values for total concentrations of asialo-LDL were 240 mg,L (180-310 mg/L) and 220 mg/L (186-390 mg/L), respectively (P = 0.82). When expressed as a percentage of serum apo B-100, median (IQR) values were 18% (16-23%) and 19% (15-29%), respectively (P = 0.78). These results suggest asialo-LDL has little value as a risk factor for coronary atherosclerosis.
authors.affiliation: School of Biology, Medical Biology Centre, The Queen's University of Belfast, Northern Ireland, UK. a.mcdowell@qub.ac.uk
authors.forename: A
authors.initials: A
authors.lastname: Mcdowell
authors.forename: I S
authors.initials: IS
authors.lastname: Young
authors.forename: G B
authors.initials: GB
authors.lastname: Wisdom
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D000906:Antibodies / Q000276:immunology; D001055:Apolipoproteins B / Q000097:blood / Q000276:immunology; D015415:Biomarkers; D008076:Cholesterol, HDL / Q000097:blood; D008078:Cholesterol, LDL / Q000097:blood* / Q000737:chemistry / Q000378:metabolism*; D003324:Coronary Artery Disease / Q000097:blood* / Q000175:diagnosis; D005260:Female; D006031:Glycosylation; D006801:Humans; D007118:Immunoassay / Q000379:methods* / Q000592:standards; D037102:Lectins / Q000276:immunology; D008297:Male; D008875:Middle Aged; D037121:Plant Lectins; D012307:Risk Factors; D012756:Sheep; D014280:Triglycerides / Q000097:blood
chemical_list: D000906:Antibodies; D001055:Apolipoproteins B; D015415:Biomarkers; D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D037102:Lectins; D037121:Plant Lectins; C017707:Ricinus communis agglutinin-1; D014280:Triglycerides