title: Effects of calcium channel antagonists on the corpora allata of adult male loreyi leafworm Mythimna loreyi: juvenile hormone acids release and intracellular calcium level.
abstract: The effects of voltage-dependent calcium channel (VDCC) antagonists and the non-specific calcium channel antagonists on both juvenile hormone acids (JHA) release and cytosolic free calcium concentration ([Ca2+]i) are investigated in the corpora allata (CA) of the adult males loreyi leafworm Mythimna loreyi. The VDCC antagonists used in this study are: the L-type antagonists diltiazem, nifedipine, and verapamil, the N-type antagonist omega-Conotoxin (CgTx) GVIA, the N- and P/Q-type antagonist omega-CgTx MVIIC, and the T-type antagonist amiloride. The non-specific calcium channel antagonists used in this study were cadmium (Cd2+), cobalt (Co2+), nickle (Ni2+), and lanthanum (La3+). The results show that both the DHPs-sensitive L-type antagonist nifedipine and the N-type antagonist omega-CgTx GVIA were able to inhibit JHA release, but only omega-CgTx GVIA was able to reduce the [Ca2+]i. Among the non-specific calcium channel antagonists, Cd2+ is the most potent in reducing JHA release but without obvious effect on the [Ca2+]i, La3+ significantly increases the [Ca2+]i but without effect on JHA release.
authors.affiliation: Department of Entomology, National Taiwan University,Taipei, Taiwan, ROC.
authors.forename: Y C
authors.initials: YC
authors.lastname: Hsieh
authors.forename: E L
authors.initials: EL
authors.lastname: Hsu
authors.forename: Y S
authors.initials: YS
authors.lastname: Chow
authors.forename: R
authors.initials: R
authors.lastname: Kou
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D015220:Calcium Channels / Q000187:drug effects; D003335:Corpora Allata / Q000187:drug effects* / Q000502:physiology*; D007605:Juvenile Hormones / Q000378:metabolism; D007915:Lepidoptera / Q000187:drug effects* / Q000502:physiology*; D008297:Male; D008670:Metals / Q000494:pharmacology; D009543:Nifedipine / Q000494:pharmacology; D020866:omega-Conotoxin GVIA / Q000494:pharmacology
chemical_list: D002121:Calcium Channel Blockers; D015220:Calcium Channels; D007605:Juvenile Hormones; D008670:Metals; D020866:omega-Conotoxin GVIA; D009543:Nifedipine; D002118:Calcium