title: Decay-accelerating factor (DAF/CD55) is a functional active element of the LPS receptor complex.
abstract: Previously, we identified an 80 kDa membrane protein (LMP80) that is capable of binding to LPS and lipid A in the presence of LBP and sCD14. LMP80 could also be detected after immuno-coprecipitation of cell membranes with LPS and lipid A, indicating a physical contact of LMP80 and LPS/lipid A. Further analysis and peptide sequencing revealed that LMP80 is identical to CD55 (decay accelerating factor, DAF), a regulatory molecule of the complement cascade. Transfection of LPS-hyporesponsive Chinese hamster ovary (CHO) cells with human CD55 resulted in the translocation of NF-B upon stimulation with LPS or lipid A. Our results demonstrate a new functional role of CD55 as a molecule able to mediate LPS-induced activation of cells that may be part of a multimeric LPS receptor complex.
authors.affiliation: Center for Medicine and Biosciences, Research Center Borstel, Borstel, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Heine
authors.forename: A J
authors.initials: AJ
authors.lastname: Ulmer
authors.forename: V T
authors.initials: VT
authors.lastname: El-Samalouti
authors.forename: A
authors.initials: A
authors.lastname: Lentschat
authors.forename: L
authors.initials: L
authors.lastname: Hamann
mesh_terms: D000818:Animals; D001692:Biological Transport; D018958:CD55 Antigens / Q000145:classification / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D016466:CHO Cells; D006224:Cricetinae; D018950:Lipopolysaccharide Receptors / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism; D014162:Transfection
chemical_list: D018958:CD55 Antigens; D018950:Lipopolysaccharide Receptors; D016328:NF-kappa B