Activation of human monocyte cell line U937 via cell surface calreticulin.
U937 cells were found to be activated by an antibacterial peptide, KLKLLLLLKLK-NH2 (L5), to generate superoxide anion (O2-)-like peripheral neutrophils. However, the state of cell surface calreticulin, a possible receptor for L5, was suggested to differ between neutrophils and U937 cells. Unlike the former, the latter ones were activated by anti-C-domain peptide antibody of calreticulin even in the absence of L5 and generated O2- in a GTP-binding protein (G-protein)-dependent manner.
Cho
J H
JH
Natori Special Laboratory, Institute of Physical and Chemical Research (RIKEN), Saitama, Japan. natori@postman.riken.go.gp
Homma
K J
KJ
Kanegasaki
S
S
Natori
S
S
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000890:Anti-Infective Agents / Q000494:pharmacology; D000906:Antibodies / Q000276:immunology / Q000302:isolation & purification; D015153:Blotting, Western; D002135:Calcium-Binding Proteins / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D037282:Calreticulin; D002462:Cell Membrane / Q000378:metabolism; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D008262:Macrophage Activation; D018832:Molecular Chaperones / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D009000:Monocytes / Q000187:drug effects / Q000276:immunology*; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism; D009842:Oligopeptides / Q000494:pharmacology; D010100:Oxygen / Q000378:metabolism; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000276:immunology; D017434:Protein Structure, Tertiary; D011817:Rabbits; D012261:Ribonucleoproteins / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D014212:Tretinoin / Q000494:pharmacology; D020298:U937 Cells
D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D000906:Antibodies; D002135:Calcium-Binding Proteins; D037282:Calreticulin; D018832:Molecular Chaperones; D009842:Oligopeptides; D010446:Peptide Fragments; D012261:Ribonucleoproteins; C096884:nysyl-leucyl-lysyl-leucyl-leucyl-leucyl-leucyl-leucyl-lysyl-leucyl-lysinamide; D014212:Tretinoin; D010100:Oxygen