[Molecular characterization of autochthonous clinical isolates of Histoplasma capsulatum var. capsulatum using RAPD-PCR].
The aim of our work was to determine the genotypic relatedness among H. capsulatum isolates from Argentinian patients with or without AIDS and isolates from other sources and geographic origins. We analyzed band patterns of 16 isolates obtained by RAPD-PCR, 11 Argentinian clinical isolates (8 from patients with AIDS and 3 from patients without AIDS), 2 strains from the CDC, 2 isolates from Mexico and one isolate from Uruguay. With the band patterns obtained, we built a dendrogram, which showed 3 major groups. Group I contained 2 isolates from Mexico, group II 1 strain from CDC and group III included 13 isolates, and was divided into 4 subgroups. Subgroup IIIa contained 2 isolates from patients with and without AIDS. Subgroup IIIb included isolates from patients without AIDS from Chaco. Subgroup IIIc contained 7 isolates from Argentina (from Buenos Aires province) and the Uruguayan isolate (6 isolates from patients with AIDS). Subgroup IIId included only one strain from CDC. All the Argentinian and the Uruguayan isolates went into group III, subgroups IIIa, IIIb and IIIc. This is a preliminary study; however, a relatedness of the RAPD-PCR band patterns and the geographic origin was observed. Although there might be a probable association between immunocompromised patients with AIDS and infective capability of some H. capsulatum isolates, further studies with a larger number of isolates from different sources and geographic origins are necessary to confirm these results.
Perrotta
D
D
Departamento de Micología, INEI ANLIS Dr. C. G. Malbrán, Av. Vélez Sarsfield 563, 1281 Buenos Aires, Argentina. dperrotta@anlis.gov.ar
Abrantes
R
R
Canteros
C
C
Rodero
L
L
Davel
G
G
D017088:AIDS-Related Opportunistic Infections / Q000453:epidemiology / Q000382:microbiology; D000818:Animals; D001118:Argentina / Q000453:epidemiology; D002685:Chiroptera / Q000469:parasitology; D006658:Histoplasma / Q000145:classification / Q000302:isolation & purification*; D006660:Histoplasmosis / Q000453:epidemiology / Q000382:microbiology* / Q000662:veterinary; D006801:Humans; D016867:Immunocompromised Host; D008800:Mexico; D008954:Models, Biological; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D019105:Random Amplified Polymorphic DNA Technique; D014583:Uruguay