Biological and molecular characterization of a pigeon paramyxovirus type-1 isolate found in Argentina.
In this report, we describe the biological and molecular characterization of a paramyxovirus type-1 (PPMV-1) isolate found in wild pigeons in an urban habitat in Buenos Aires, Argentina. Of the nine pigeons captured, three were moribund, and the other six showed diarrhea, ataxia, tremor, torticolis, and wing paralysis. The intracerebral pathogenicity index was 1.29, and the amino acid (aa) sequence at the fusion protein cleavage site was 112GRQ KRF117. These characteristics correspond to a virulent Newcastle disease virus isolate. Nevertheless, it was not possible to reproduce the disease in chickens experimentally although the chickens exhibited seroconversion after inoculation. On the other hand, pigeons inoculated with the isolate became sick. These results provide further evidence about the unusual pathogenicity of PPMV-1 for chickens and show once more the need for more biological determinations in these cases to arrive at a final conclusion.
Zanetti
F
F
Instituto de Biotecnología, CNIA, INTA, Castelar, Buenos Aires, Argentina.
Mattiello
R
R
Garbino
C
C
Kaloghlian
A
A
Terrera
M V
MV
Boviez
J
J
Palma
E
E
Carrillo
E
E
Berinstein
A
A
D000595:Amino Acid Sequence; D000818:Animals; D000835:Animals, Wild; D001118:Argentina; D010856:Columbidae / Q000821:virology*; D004279:DNA, Viral / Q000032:analysis; D006385:Hemagglutination Inhibition Tests / Q000662:veterinary; D006386:Hemagglutination Tests / Q000662:veterinary; D008969:Molecular Sequence Data; D009521:Newcastle Disease / Q000473:pathology / Q000821:virology*; D009522:Newcastle disease virus / Q000235:genetics* / Q000472:pathogenicity; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment / Q000662:veterinary; D014760:Viral Fusion Proteins / Q000737:chemistry / Q000235:genetics; D014774:Virulence
D004279:DNA, Viral; D014760:Viral Fusion Proteins