title: Adhesion and proliferation of rat vascular smooth muscle cells (VSMC) on polyethylene implanted with O+ and C+ ions.
abstract: Polyethylene was implanted with 30-keV oxygen (PE/O+) or 23-keV carbon ions (PE/C+) at 10(13) to 5 x 10(15) ions cm(-2) doses in order to improve the adhesion of vascular smooth muscle cell (VSMC) to the polymer surface in vitro because of its oxidation and carbon-enrichment. The concentration of -CO- groups in the PE/O+ and PE/C+ samples increased only up to doses of 3 x 10(14) and 10(15) ions cm(-2), respectively, and then declined. At the same time, the concentration of these groups, measured at a dose of 3 x 10(14) ions cm(-2), was higher in PE/O+ than in PE/C+ samples. Similarly, the number of initially-adhering rat VSMC (24 h after seeding) increased only up to a dose of 3 x 10(13) and 10(15) ions cm(-2) on PE/O+ and PE/C+ samples, respectively. In addition, between doses of 10(13) and 10(14) ions cm(-2), this number was about two to three times higher on PE/O+ samples. On the other hand, the surface wettability increased proportionally to the implanted ion dose, especially above a dose of 10(14) ions cm(-2). Thus, the number of initially-adhered cells appeared to be positively correlated with the amount of the oxygen group present at the polymer surface rather than with the surface wettability. The higher cell adhesion was accompanied by adsorption of fluorescent dye-conjugated collagen IV in larger amounts. The highest numbers of initially-adhered cells were usually associated with the lowest rates of subsequent proliferation (measured by the doubling time, BrdU labelling and M
authors.affiliation: Institute of Physiology, Academy of Sciences of the Czech Republic, Prague. lucy@biomed.cas.cz
authors.forename: L
authors.initials: L
authors.lastname: Bacáková
authors.forename: K
authors.initials: K
authors.lastname: Walachová
authors.forename: V
authors.initials: V
authors.lastname: Svorcík
authors.forename: V
authors.initials: V
authors.lastname: Hnatowicz
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D002244:Carbon / Q000737:chemistry; D002412:Cations / Q000737:chemistry; D002448:Cell Adhesion; D002455:Cell Division; D024141:Collagen Type IV / Q000493:pharmacokinetics; D004563:Electrochemistry; D009131:Muscle, Smooth, Vascular / Q000166:cytology* / Q000187:drug effects; D010100:Oxygen / Q000737:chemistry; D011095:Polyethylenes / Q000737:chemistry* / Q000378:metabolism* / Q000494:pharmacology; D051381:Rats; D013329:Structure-Activity Relationship; D013499:Surface Properties; D017155:Wettability
chemical_list: D001672:Biocompatible Materials; D002412:Cations; D024141:Collagen Type IV; D011095:Polyethylenes; D002244:Carbon; D010100:Oxygen