Isolation and characterization of photosystem II subcomplexes from cyanobacteria lacking photosystem I.
A photosystem II (PSII) core complex lacking the internal antenna CP43 protein was isolated from the photosystem II of Synechocystis PCC6803, which lacks photosystem I (PSI). CP47-RC and reaction centre (RCII) complexes were also obtained in a single procedure by direct solubilization of whole thylakoid membranes. The CP47-RC subcore complex was characterized by SDS/PAGE, immunoblotting, MALDI MS, visible and fluorescence spectroscopy, and absorption detected magnetic resonance. The purity and functionality of RCII was also assayed. These preparations may be useful for mutational analysis of PSII RC and CP47-RC in studying primary reactions of oxygenic photosynthesis.
Szabò
I
I
Department of Biology, University of Tuscia, Viterbo, Italy.
Rigoni
F
F
Bianchetti
M
M
Carbonera
D
D
Pierantoni
F
F
Seraglia
R
R
Segalla
A
A
Giacometti
G M
GM
D015153:Blotting, Western; D002853:Chromatography, Liquid; D000458:Cyanobacteria / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry / Q000302:isolation & purification*; D013050:Spectrometry, Fluorescence; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D020524:Thylakoids / Q000737:chemistry
D045322:Photosynthetic Reaction Center Complex Proteins