A simple method for the assay of colistin in human plasma, using pre-column derivatization with 9-fluorenylmethyl chloroformate in solid-phase extraction cartridges and reversed-phase high-performance liquid chromatography.
A simple, selective and sensitive high-performance liquid chromatographic (HPLC) method is described for the determination of colistin in human plasma. Derivatization with 9-fluorenylmethyl chloroformate was performed in the same solid-phase extraction C18 cartridge used for sample pre-treatment, followed by reversed-phase HPLC with fluorimetric detection. Quantification was achieved using the ratio of the summed peak areas of colistin A and B derivatives to that of the derivative of netilmicin (internal standard). Linear calibration curves were obtained within the concentrations of colistin sulfate from 0.10 to 4.0 mg/l in plasma. Accuracy was within 10% and reproducibility (RSD) was less than 10%.
Li
J
J
Centre for Pharmaceutical Research, University of South Australia, Adelaide Australia.
Milne
R W
RW
Nation
R L
RL
Turnidge
J D
JD
Coulthard
K
K
Johnson
D W
DW
D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003091:Colistin / Q000097:blood*; D005449:Fluorenes / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D013058:Mass Spectrometry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D005449:Fluorenes; D005456:Fluorescent Dyes; C054007:1-(9-fluorenyl)methyl chloroformate; D003091:Colistin