title: Morphological and immunohistochemical changes in splenic macrophages of pigs infected with classical swine fever.
abstract: Classical swine fever (CSF) was induced in 20 pigs by inoculation with a virulent strain of CSF virus to determine sequential changes (2, 4, 7, 10 and 14 days post-inoculation) in the number and morphology of splenic macrophages (red pulp and lymphoid marginal zone) and thus to assess the role of these cells in the pathogenesis of the disease. The first splenic cells to be infected with CSF virus were macrophages in the marginal zone followed by other macrophage populations. The initial phase of CSF was associated with an increase in splenic macrophage numbers in the marginal zone and a decrease in the red pulp. Subsequently, the numbers in the red pulp increased. The study suggested that infection, mobilization and apoptosis of splenic macrophages play an important role in the spread of CSF virus in vivo. Moreover, the secretory changes that occurred in macrophages in the initial phase of the infection suggested that macrophages release chemical mediators capable of modulating pathogenesis.
authors.affiliation: Departamento Anatomía Patológica, Facultad de Veterinaria, Campus Universitario de Rabaneles, 14014 Córdoba, Spain.
authors.forename: J C
authors.initials: JC
authors.lastname: Gómez-Villamandos
authors.forename: E
authors.initials: E
authors.lastname: Ruiz-Villamor
authors.forename: M J
authors.initials: MJ
authors.lastname: Bautista
authors.forename: C P
authors.initials: CP
authors.lastname: Sánchez
authors.forename: P J
authors.initials: PJ
authors.lastname: Sánchez-Cordón
authors.forename: F J
authors.initials: FJ
authors.lastname: Salguero
authors.forename: A
authors.initials: A
authors.lastname: Jover
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000276:immunology; D015415:Biomarkers / Q000032:analysis; D002452:Cell Count / Q000662:veterinary; D006691:Classical Swine Fever / Q000209:etiology / Q000378:metabolism / Q000473:pathology*; D006692:Classical Swine Fever Virus / Q000302:isolation & purification / Q000502:physiology* / Q000648:ultrastructure; D022162:Cytoplasmic Vesicles / Q000648:ultrastructure; D005260:Female; D007124:Immunoenzyme Techniques / Q000662:veterinary; D007181:Inclusion Bodies, Viral / Q000648:ultrastructure; D008264:Macrophages / Q000378:metabolism / Q000473:pathology*; D008297:Male; D013154:Spleen / Q000473:pathology*; D013997:Time Factors
chemical_list: D000956:Antigens, Viral; D015415:Biomarkers