title: Molecular cloning and characterization of mouse Tspan-3, a novel member of the tetraspanin superfamily, expressed on resting dendritic cells.
abstract: Dendritic cells (DCs) are the most potent antigen-presenting cells and play an essential role for triggering T-cell-mediated immune responses. In search for novel cell surface molecules expressed on DCs involved in T cell priming by representational differential analysis, we identified a mouse homologue of Tspan-3 (mTspan-3), a novel member of the tetraspanin superfamily. The mTspan-3 consists of four hydrophobic, putative transmembrane regions, forming a small and a large extracellular loop, with short intracellular amino and carboxil tails. Although the mTspan-3 is expressed on a variety of immune cell types including resting DCs, its expression on DCs is downregulated during activation induced by cross-linking CD40 with anti-CD40 monoclonal antibody. These results suggest that mTspan-3 may be involved in the function of DCs in association with T cell stimulation.
authors.affiliation: Department of Immunology, Institute of Basic Medical Sciences, Institute of Clinical Medicine, 1-1-1 Tennodai, Tsukuba Science City, Ibaraki 305-8575, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Tokoro
authors.forename: K
authors.initials: K
authors.lastname: Shibuya
authors.forename: M
authors.initials: M
authors.lastname: Osawa
authors.forename: S
authors.initials: S
authors.lastname: Tahara-Hanaoka
authors.forename: A
authors.initials: A
authors.lastname: Iwama
authors.forename: T
authors.initials: T
authors.lastname: Kitamura
authors.forename: H
authors.initials: H
authors.lastname: Nakauchi
authors.forename: A
authors.initials: A
authors.lastname: Shibuya
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D003001:Cloning, Molecular; D003713:Dendritic Cells / Q000276:immunology*; D015536:Down-Regulation; D008213:Lymphocyte Activation; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000096:biosynthesis; D017386:Sequence Homology, Amino Acid; D013601:T-Lymphocytes / Q000276:immunology; D014018:Tissue Distribution; D014158:Transcription, Genetic
chemical_list: D008565:Membrane Proteins; D012333:RNA, Messenger