Expression of p57/Kip2 protein in pancreatic adenocarcinoma.
BACKGROUND
Evaluation of the biologic character of carcinomas requires understanding of cell cycle regulators.


OBJECTIVE
To investigate p57 expression in human pancreatic adenocarcinoma and cyst adenoma.


METHODS
We examined the expression of p57(Kip2), a member of the Cip/Kip family, in 45 pancreatic adenocarcinomas, 7 cystadenomas, and 7 chronic pancreatitis cases.


RESULTS
The p57 labeling index (LI) in duct epithelia in chronic pancreatitis averaged 32.8+/-8.3 and was significantly higher than in normal duct epithelia (18.8+/-6.6; p = 0.0011). For the carcinoma, the LI averaged 46.0+/-20.9, which was significantly higher than that for normal duct epithelia (p < 0.0001) and cystadenoma (16.0 11.2; p = 0.0007). However, it was significantly reduced in cases with stage IV disease (p = 0.0351), lymph node metastasis (p = 0.0003), larger size (p = 0.0094), capsular invasion (p = 0.0462), lymphatic invasion (p = 0.0351), and cell proliferating activity (p = 0.0002). In multivariate analysis, p57 LI in pancreatic adenocarcinoma was independently linked to high proliferating activity (p = 0.0230).


CONCLUSIONS
These results suggest that p57 plays a role in the hyperplastic change of the ducts in chronic pancreatitis and that pS7 overexpression contributes to the downregulation of cell proliferation, and its decreased expression contributes to the progression of pancreatic adenocarcinoma.
Ito
Y
Y
Department of Surgery, Osaka Seamen's Insurance Hospital, Osaka, Japan.
Takeda
T
T
Wakasa
K
K
Tsujimoto
M
M
Matsuura
N
N
D000230:Adenocarcinoma / Q000737:chemistry* / Q000473:pathology; D000328:Adult; D000368:Aged; D002455:Cell Division; D002908:Chronic Disease; D050761:Cyclin-Dependent Kinase Inhibitor p57; D003537:Cystadenoma / Q000737:chemistry / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000032:analysis; D008297:Male; D008875:Middle Aged; D009687:Nuclear Proteins / Q000032:analysis*; D010190:Pancreatic Neoplasms / Q000737:chemistry* / Q000473:pathology; D010195:Pancreatitis / Q000378:metabolism / Q000473:pathology
C493964:CDKN1C protein, human; D050761:Cyclin-Dependent Kinase Inhibitor p57; D019394:Ki-67 Antigen; D009687:Nuclear Proteins