Lung lesions and anti-ulcer agents beneficial effect: anti-ulcer agents pentadecapeptide BPC 157, ranitidine, omeprazole and atropine ameliorate lung lesion in rats.
Anti-ulcer agents may likely attenuate lesions outside the gastrointestinal tract, since they had protected gastrectomized rats (a "direct cytoprotective effect"). Therefore, their therapeutic potential in lung/stomach lesions were shown. Rats received an intratracheal (i.t.) HCl instillation [1.5 ml/kg HCl (pH 1.75)] (lung lesion), and an intragastric (i.g.) instillation of 96% ethanol (gastric lesion; 1 ml/rat, 24 h after i.t. HCl instillation), then sacrificed 1 h after ethanol. Basically, in lung-injured rats, the subsequent ethanol-gastric lesion was markedly aggravated. This aggravation, however, in turn, did not affect the severity of the lung lesions in the further period, at least for 1 h of observation. Taking intratracheal HCl-instillation as time 0, a gastric pentadecapeptide, GEPPPGKPADDAGLV, M.W.1419, coded BPC 157 (10 microg, 10 ng, 10 pg), ranitidine (10 mg), atropine (10 mg), omeprazole (10 mg), were given [/kg, intraperitoneally (i.p.)] (i) once, only prophylactically [as a pre-treatment (at -1h)], or as a co-treatment [at 0)], or only therapeutically (at +18h or +24 h); (ii) repeatedly, combining prophylactic/therapeutic regimens [(-1 h)+(+24 h)] or [(0)+(+24 h)], or therapeutic/therapeutic regimens [(+18 h)+(+24 h)]. For all agents, combining their prophylactic and salutary regimens (at -1 h/+24 h, or at 0/+24 h) attenuated lung lesions; even if effect had been not seen already with a single application, it became prominent after repeated treatment. In single application studies, relative to controls, a co-treatment (except to omeprazole), a pre-treatment (at -1 h) (pentadecapeptide BPC 157 and atropine, but not ranitidine and omeprazole) regularly attenuated, while therapeutically, atropine (at +18 h), pentadecapeptide BPC 157 highest dose and omeprazole (at +24 h), reversed the otherwise more severe lung lesions.
Stancic-Rokotov
D
D
Clinic for Thoracic Surgery Jordanovac, Medical Faculty University of Zagreb, Zagreb, Croatia.
Slobodnjak
Z
Z
Aralica
J
J
Aralica
G
G
Perovic
D
D
Staresinic
M
M
Gjurasin
M
M
Anic
T
T
Zoricic
I
I
Buljat
G
G
Prkacin
I
I
Sikiric
P
P
Seiwerth
S
S
Rucman
R
R
Petek
M
M
Turkovic
B
B
Kokic
N
N
Jagic
V
V
Boban-Blagaic
A
A
D000818:Animals; D000897:Anti-Ulcer Agents / Q000627:therapeutic use*; D001285:Atropine / Q000627:therapeutic use*; D006851:Hydrochloric Acid; D008171:Lung Diseases / Q000139:chemically induced / Q000188:drug therapy* / Q000517:prevention & control*; D008297:Male; D009853:Omeprazole / Q000627:therapeutic use*; D010446:Peptide Fragments / Q000627:therapeutic use*; D011506:Proteins / Q000627:therapeutic use*; D011899:Ranitidine / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar
D000897:Anti-Ulcer Agents; D010446:Peptide Fragments; D011506:Proteins; D001285:Atropine; D011899:Ranitidine; C083833:BPC 157; D009853:Omeprazole; D006851:Hydrochloric Acid