Free radical scavenging active components from Cedrus deodara.
An activity-directed fractionation and purification process was used to identify the antioxidant components of Cedrus deodara. Dried heartwood powder of C. deodara was first defatted with petroleum ether and then extracted with chloroform. The chloroform extract showed strong antioxidant activity on 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical. This fraction was then subjected to separation and purification using silica gel column chromatography. Three compounds with potent antioxidant activity were isolated in significant yields and identified by spectroscopic methods ((1)H NMR, (13)C NMR, IR, and MS). They were identified as (-)-matairesinol, (-)-nortrachelogenin, and a dibenzylbutyrolactollignan (4,4',9-trihydroxy-3,3'-dimethoxy-9,9'-epoxylignan). This is the first report of the occurrence of these compounds in C. deodara.
Tiwari
A K
AK
Division of Pharmacology and Natural Product Laboratory and Division of Organic Chemistry-I, Indian Institute of Chemical Technology, Hyderabad 500 007, India.
Srinivas
P V
PV
Kumar
S P
SP
Rao
J M
JM
D000975:Antioxidants / Q000032:analysis* / Q000302:isolation & purification; D028203:Cedrus / Q000737:chemistry*; D005591:Chemical Fractionation; D002725:Chloroform; D002850:Chromatography, Gel; D016166:Free Radical Scavengers; D005663:Furans / Q000032:analysis; D017705:Lignans / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy
C439406:4,4',9-trihydroxy-3,3'-dimethoxy-9,9'-epoxylignan; D000975:Antioxidants; D016166:Free Radical Scavengers; D005663:Furans; D017705:Lignans; C068935:matairesinol; C020436:nortrachelogenin; D002725:Chloroform