title: Tegumental alterations in juvenile Schistosoma haematobium harboured in hamsters following artemether treatment.
abstract: We report the findings of a detailed temporal study on tegumental alterations in juvenile Schistosoma haematobium, induced by artemether, using scanning electron microscopy. Hamsters infected with S. haematobium cercariae for 28 days were treated intragastrically with a single dose of 300 mg/kg artemether. Groups of two hamsters were killed 24 h, 72 h and 7 days after treatment, and schistosomula were recovered from livers by perfusion and subsequent systematic examination of the tissue, before routinely processing for scanning electron microscopic examination. Most schistosomula collected 24 h after artemether administration showed severe tegumental damage, usually including swelling, fusion, vesiculation, peeling and collapse of enlarged sensory structures. After 72 h, tegumental damage had increased and schistosomula generally showed contraction with extensive swelling, erosion and peeling of the tegument. Seven days post-treatment, severe tegumental damage was only seen in a single male specimen with swelling of the worm body and destruction of the oral sucker. The other schistosomula showed only light to moderate damage, suggesting that schistosomula surviving the treatment began to recover. Our findings of tegumental damage following artemether treatment correlate with the efficacy of this novel antischistosomal drug in killing the juvenile stages of S. haematobium and complement recent findings with S. japonicum and S. mansoni.
authors.affiliation: Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine, Shanghai 200025, China.
authors.forename: S
authors.initials: S
authors.lastname: Xiao
authors.forename: B
authors.initials: B
authors.lastname: Shen
authors.forename: J
authors.initials: J
authors.lastname: Chollet
authors.forename: J
authors.initials: J
authors.lastname: Utzinger
authors.forename: M
authors.initials: M
authors.lastname: Tanner
mesh_terms: D000818:Animals; D000077549:Artemether; D037621:Artemisinins; D006224:Cricetinae; D005260:Female; D008297:Male; D008855:Microscopy, Electron, Scanning; D012548:Schistosoma haematobium / Q000187:drug effects* / Q000648:ultrastructure*; D012553:Schistosomiasis haematobia / Q000188:drug therapy* / Q000469:parasitology; D012556:Schistosomicides / Q000494:pharmacology*; D012717:Sesquiterpenes / Q000494:pharmacology* / Q000627:therapeutic use
chemical_list: D037621:Artemisinins; D012556:Schistosomicides; D012717:Sesquiterpenes; D000077549:Artemether