title: T cell clones from Schistosoma haematobium infected and exposed individuals lacking distinct cytokine profiles for Th1/Th2 polarisation.
abstract: T cell clones were derived from peripheral blood mononuclear cells of Schistosoma haematobium infected and uninfected individuals living in an endemic area. The clones were stimulated with S. haematobium worm and egg antigens and purified protein derivative. Attempts were made to classify the T cell clones according to production of the cytokines IL-4, IL-5 and IFN-gamma. All the T cell clones derived were observed to produce cytokines used as markers for the classification of Th1/Th2 subsets. However, the 'signature' cytokines marking each subset were produced at different levels. The classification depended on the dominating cytokine type, which was having either Th0/1 or Th0/2 subsets. The results indicated that no distinct cytokine profiles for polarisation of Th1/Th2 subsets were detected in these S. haematobium infected humans. The balance in the profiles of cytokines marking each subset were related to infection and re-infection status after treatment with praziquantel. In the present study, as judged by the changes in infection status with time, the T cell responses appeared to be less stable and more dynamic, suggesting that small quantitative changes in the balance of the cytokines response could result in either susceptibility or resistant to S. haematobium infection.
authors.affiliation: Biochemistry Department, University of Zimbabwe, Harare, Zimbabwe. mduluza@blair.co.zw
authors.forename: T
authors.initials: T
authors.lastname: Mduluza
authors.forename: P D
authors.initials: PD
authors.lastname: Ndhlovu
authors.forename: N
authors.initials: N
authors.lastname: Midzi
authors.forename: C
authors.initials: C
authors.lastname: Mary
authors.forename: C P
authors.initials: CP
authors.lastname: Paris
authors.forename: C M
authors.initials: CM
authors.lastname: Turner
authors.forename: S K
authors.initials: SK
authors.lastname: Chandiwana
authors.forename: M E
authors.initials: ME
authors.lastname: Woolhouse
authors.forename: A J
authors.initials: AJ
authors.lastname: Dessein
authors.forename: P
authors.initials: P
authors.lastname: Hagan
mesh_terms: D000818:Animals; D000871:Anthelmintics / Q000627:therapeutic use; D002460:Cell Line; D002648:Child; D004797:Enzyme-Linked Immunosorbent Assay; D005500:Follow-Up Studies; D006801:Humans; D007371:Interferon-gamma / Q000032:analysis / Q000096:biosynthesis*; D015847:Interleukin-4 / Q000032:analysis / Q000096:biosynthesis*; D015848:Interleukin-5 / Q000032:analysis / Q000096:biosynthesis*; D010270:Parasite Egg Count; D011223:Praziquantel / Q000627:therapeutic use; D012548:Schistosoma haematobium / Q000276:immunology*; D012553:Schistosomiasis haematobia / Q000188:drug therapy / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000145:classification / Q000378:metabolism; D006377:T-Lymphocytes, Helper-Inducer / Q000145:classification* / Q000378:metabolism; D018417:Th1 Cells / Q000145:classification / Q000378:metabolism; D018418:Th2 Cells / Q000145:classification / Q000378:metabolism; D017186:Titrimetry
chemical_list: D000871:Anthelmintics; D015848:Interleukin-5; D015847:Interleukin-4; D011223:Praziquantel; D007371:Interferon-gamma