Dietary vitamin E supplementation protects the rat large intestine from experimental inflammation.
Vitamin E, the most potent antioxidant in the lipid phase, was tested for antiinflammatory activity in trinitrobenzenesulfonic acid-induced rat colitis. Rats were fed a nonpurified diet (saline and control groups) or a vitamin E supplemented diet (treated group, 300 mg/kg nonpurified diet). Vitamin E supplementation, which resulted in increased colonic vitamin E levels, reduced colonic weight and damage score, prevented lipid peroxidation and diarrhea, reduced interleukin-1 beta levels and preserved glutathione reductase activity and total glutathione levels. However, it did not modify myeloperoxidase levels, which are indicative of neutrophil infiltration in the inflamed colon. Vitamin E protects the rat colon from oxidative stress associated with inflammation.
González
R
R
Department of Pharmacology, School of Pharmacy, University of Granada, Campus de Cartuja s/n, 18071 Granada, Spain.
Sánchez de Medina
F
F
Gálvez
J
J
Rodríguez-Cabezas
M E
ME
Duarte
J
J
Zarzuelo
A
A
D000818:Animals; D003092:Colitis / Q000139:chemically induced / Q000517:prevention & control*; D019587:Dietary Supplements; D005260:Female; D005978:Glutathione / Q000032:analysis; D005980:Glutathione Reductase / Q000032:analysis; D007375:Interleukin-1 / Q000032:analysis; D007420:Intestine, Large / Q000187:drug effects / Q000473:pathology*; D018384:Oxidative Stress; D009195:Peroxidase / Q000032:analysis; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D014810:Vitamin E / Q000008:administration & dosage* / Q000494:pharmacology
D007375:Interleukin-1; D014810:Vitamin E; D009195:Peroxidase; D005980:Glutathione Reductase; D005978:Glutathione