Growth kinetics rather than stress accelerate telomere shortening in cultures of human diploid fibroblasts in oxidative stress-induced premature senescence.
WI-38 human diploid fibroblasts underwent accelerated telomere shortening (490 bp/stress) and growth arrest after exposure to four subcytotoxic 100 microM tert-butylhydroperoxide (t-BHP) stresses, with a stress at every two population doublings (PD). After subcytotoxic 160 microM H2O2 stress or five repeated 30 microM t-BHP stresses along the same PD, respectively a 322 +/- 55 and 380 +/- 129 bp telomere shortening was observed only during the first PD after stress. The percentage of cells resuming proliferation after stress suggests this telomere shortening is due to the number of cell divisions accomplished to reach confluence during the first PD after stress.
Dumont
P
P
Department of Biology, University of Namur, Belgium.
Royer
V
V
Pascal
T
T
Dierick
J F
JF
Chainiaux
F
F
Frippiat
C
C
de Magalhaes
J P
JP
Eliaers
F
F
Remacle
J
J
Toussaint
O
O
D002455:Cell Division / Q000187:drug effects / Q000502:physiology*; D016922:Cellular Senescence / Q000502:physiology*; D004171:Diploidy; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007700:Kinetics; D018384:Oxidative Stress; D016615:Telomere / Q000378:metabolism*; D013936:Thymidine / Q000737:chemistry; D001616:beta-Galactosidase / Q000378:metabolism; D020122:tert-Butylhydroperoxide / Q000494:pharmacology
D020122:tert-Butylhydroperoxide; D006861:Hydrogen Peroxide; D001616:beta-Galactosidase; D013936:Thymidine