Comparison of the chiral resolution of econazole, miconazole, and sulconazole by HPLC using normal-phase amylose CSPs.
Resolution of the enantiomers of (+/-)-econazole, (+/-)-miconazole, and (+/-)-sulconazole has been achieved on different normal-phase chiral amylose columns, Chi-ralpak AD, AS, and AR. The mobile phase used was hexane-2-propanol-diethylamine, 400:99:1 (v/v). The flow rates of the mobile phase used were 0.50 and 1.00 mL min(-1). The alpha values for the resolved enantiomers of econazole, miconazole, and sulconazole on the chiral phases were in the range 1.63 to 1.04; the Rs values varied from 5.68 to 0.32.
Aboul-Enein
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Biological and Medical Research Department, King Faisal Specialist Hospital and Research Center, Riyadh, Saudi Arabia. enein@kfshrc.edu.sa
Ali
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D000688:Amylose / Q000737:chemistry; D000935:Antifungal Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004464:Econazole / Q000032:analysis; D007093:Imidazoles / Q000032:analysis; D008825:Miconazole / Q000032:analysis; D008968:Molecular Conformation
D000935:Antifungal Agents; D007093:Imidazoles; C033308:sulconazole; D004464:Econazole; D008825:Miconazole; D000688:Amylose