[Production of monoclonal antibodies to T-independent type 2 antigens with OptiMem-Iscove medium].
To obtain monoclonal antibodies to T-independent antigens of type 2, having low immunogenicity and incapable of inducing the appearance of memory cells, the use of the medium OptiMem-Iscove (1:1) with 10% of fetal serum, glutamine (50 mM) and antibiotics (100 units/ml) is proposed. The main advantage of this medium is the possibility of cloning cells without the use of feeders. The method has been approved in the process of obtaining monoclonal antibodies (McAb) to TI-2 antigens, both bacterial (S3) and synthetic (PVP), as well as to McAb to T-dependent antigen (alpha-chains of human IgA).
Sidorova
E V
EV
Research Institute of Viral Preparations, Moscow, Russia.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000955:Antigens, T-Independent / Q000276:immunology*; D003470:Culture Media; D005260:Female; D006801:Humans; D006825:Hybridomas; D007137:Immunoglobulin alpha-Chains / Q000276:immunology; D051379:Mice; D008808:Mice, Inbred CBA; D011134:Polysaccharides / Q000276:immunology; D011135:Polysaccharides, Bacterial / Q000276:immunology; D011205:Povidone; D014614:Vaccines, Synthetic / Q000276:immunology
D000911:Antibodies, Monoclonal; D000955:Antigens, T-Independent; D003470:Culture Media; D007137:Immunoglobulin alpha-Chains; D011134:Polysaccharides; D011135:Polysaccharides, Bacterial; D014614:Vaccines, Synthetic; C008986:polysaccharide S3; D011205:Povidone