[Analysis of several genes using primer extension preamplification based on individual cell].
OBJECTIVE
To analyze several genes at the same time in one individual cell using primer extension preamplification (PEP).


METHODS
The whole genome of individual cell was preamplified by PEP using 10 base random oligonucleotides. The aliquots(5 mu liter) from PEP were used to amplify SRY, ZP(3), RhCE, RhD and F VIII genes by nested PCR.


RESULTS
The five genes could be amplified following PEP-PCR. Lymphocyte of four genotypes were diagnosed by PEP-PCR; correct diagnoses were obtained in 95.6%.


CONCLUSIONS
At least five genes can be analyzed by PEP-PCR based on one individual cell in this laboratory. This method could be used in preimplantation genetic diagnosis and noninvasive prenatal diagnosis.
Ma
J
J
Reproductive Medicine Center, Changhai Hospital, the Second Military Medical University, Shanghai, 200433 P.R. China. sf2102518@newpalm.com
Ji
Y
Y
Sheng
Y
Y
Zhao
Y
Y
D005260:Female; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*