title: Age-dependent effect of domperidone on dopamine release by the hypoxic carotid body in the rabbit.
abstract: The aim of this study was to identify the mechanisms that regulate dopamine release (DA(r)) by the hypoxic carotid body (CB) during development. CBs sampled from adult (n = 58) and 10-day-old (n = 53) rabbits were incubated for 1 h in a medium equilibrated with 8% O(2) in N(2) without or in the presence of the specific DA D(2) receptor antagonist domperidone, 0.01, 0.1 and 1 microM. DA and its major metabolite DOPAC were measured in the CB (DA(CB)) and in the medium (DA(r)) by HPLC+ED. In adults, each concentration of domperidone significantly decreased DA(CB) and increased DA(r), compared with control (p < 0.01). In contrast, in 10-day old, only the 1 microM domperidone concentration decreased DA(CB) and increased DA(r) compared with control (p < 0.001). The data show that domperidone increases CB DA(r) in response to hypoxia in a concentration- and age-dependent manner and suggest this response depends, in part, on the functional maturation of CB DA D(2) receptors.
authors.affiliation: Unité de Recherche en Périnatologie, Centre Hospitalier Universitaire de Québec, Hôpital Saint-François d'Assise, Université Laval, Québec, Qué., Canada. aida.bairam@crsfa.ulaval.ca
authors.forename: A
authors.initials: A
authors.lastname: Bairam
authors.forename: R
authors.initials: R
authors.lastname: Kinkead
authors.forename: F
authors.initials: F
authors.lastname: Marchal
mesh_terms: D000375:Aging; D000818:Animals; D002344:Carotid Body / Q000187:drug effects* / Q000378:metabolism; D004294:Domperidone / Q000494:pharmacology*; D004298:Dopamine / Q000378:metabolism*; D018492:Dopamine Antagonists / Q000494:pharmacology*; D066298:In Vitro Techniques; D010100:Oxygen / Q000008:administration & dosage*; D011817:Rabbits
chemical_list: D018492:Dopamine Antagonists; D004294:Domperidone; D010100:Oxygen; D004298:Dopamine