[Effect of chitosan on the production of anthracene derivatives in tissue culture of Rheum palmatum L].
Explant cultures have been, besides other things, used for elicitation, which is an economically advantageous method making use of protective mechanism of plants to increase the production of secondary metabolites. The present study investigated the effect of 6, 12, 24, 48, 72, and 168 hours lasting action of four concentrations of the biotic elicitor chitosan on the production of anthracene derivatives by the callus and suspension culture of Rheum palmatum L. The culture was cultivated on a Murashige-Skoog medium with an addition of 10 mg.l-1 of alpha-naphthylacetic acid. A positive effect on the production was exerted in particular by elication of the suspension culture. The maximal content of anthracene derivatives (1.181%), found by photometric determination according to PhBs 4, as demonstrated after 24-hour action of a chitosan solution of a concentration of 1 mg/30 ml of the medium. In contrast to the suspension culture, the production of anthracene derivatives in the callus culture was influenced by elicitation only in the minimal way.
Kasparová
M
M
Katedra farmakognozie Farmaceutické fakulty Univerzity Karlovy, Hradec Králové. kasparova@faf.cuni.cz
Siatka
T
T
D000873:Anthracenes / Q000378:metabolism*; D002686:Chitin / Q000031:analogs & derivatives / Q000494:pharmacology*; D048271:Chitosan; D046508:Culture Techniques; D010946:Plants, Medicinal; D012250:Rheum / Q000378:metabolism*
D000873:Anthracenes; D002686:Chitin; D048271:Chitosan