Influence of laser irradiation on endogenous hyaluronan in rabbit iris and aqueous humor.
OBJECTIVE
To monitor changes of endogenous hyaluronan in the iris tissue and aqueous humor after an isolated trauma to the iris by argon laser irradiation of the anterior surface of the iris.


METHODS
Iris and aqueous hyaluronan concentrations in rabbit were measured with a radiometric assay at different time points after laser irradiation.


RESULTS
Total hyaluronan content in iris tissue increased 3-fold to a peak concentration of 71-72 microg/g at 1 and 2 days after laser treatment. Aqueous hyaluronan increased to a maximum of about 1.6 microg/ml at 2 h and 12 h after laser irradiation of the iris.


CONCLUSIONS
The iris tissue responds with increased hyluronan synthesis to an isolated iris argon laser irradiation and it seems to be the most important source of aqueous hyaluronan.
Koralewska-Makár
A
A
Department of Ophthalmology, University of Lund, Sweden. Anna.Makar@oft.lu.se
Johnsson
C
C
Bruun
A
A
Stenevi
U
U
Ehinger
B
B
D000818:Animals; D001082:Aqueous Humor / Q000378:metabolism*; D005136:Eye Proteins / Q000378:metabolism*; D006820:Hyaluronic Acid / Q000378:metabolism*; D007498:Iris / Q000378:metabolism* / Q000601:surgery; D053685:Laser Therapy; D011817:Rabbits
D005136:Eye Proteins; D006820:Hyaluronic Acid