[HPLC determination of six flavonoid constituents in Ginkgo biloba leaves].
Six flavonoid constituents (quercetin, isorhamnetin, kaempferol, bilobetin, ginkgetin and sciadopitysin) were isolated from Ginkgo biloba leaves and determined by reversed phase HPLC using salvianolic acid B as internal standard. The column employed was Zorbax ODS (150 mm x 4 mm ID, 5 microns). The mobile phase consisted of solvent A (methanol) and solvent B [tetrahydrofuran--water--formic acid (34:65:1)] for gradient elution. The flow rate was 1 ml.min-1 and detection was effected at 350 nm. This method is accurate, rapid and reproducible. Analytical data for various samples were given.
Chi
J D
JD
Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050.
He
X F
XF
Liu
A R
AR
Xu
L X
LX
D044946:Biflavonoids; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification*; D020441:Ginkgo biloba / Q000737:chemistry*; D015394:Molecular Structure; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal; D011794:Quercetin / Q000737:chemistry / Q000302:isolation & purification*
D044946:Biflavonoids; D005419:Flavonoids; C402623:bilobetin; C077458:ginkgetin; D011794:Quercetin