title: The eukaryotic gene transcription machinery.
abstract: Seven purified proteins may be combined to reconstitute regulated, promoter-dependent RNA polymerase II transcription: five general transcription factors, Mediator, and RNA polymerase II. The entire system has been conserved across species from yeast to humans. The structure of RNA polymerase II, consisting of 10 polypeptides with a mass of about 500 kDa, has been determined at atomic resolution. On the basis of this structure, that of an actively transcribing RNA polymerase II complex has been determined as well.
authors.affiliation: Department of Structural Biology, Stanford University School of Medicine, CA 94305, USA.
authors.forename: R D
authors.initials: RD
authors.lastname: Kornberg
mesh_terms: D000818:Animals; D018360:Crystallography, X-Ray; D005057:Eukaryotic Cells / Q000502:physiology*; D006801:Humans; D008958:Models, Molecular; D011487:Protein Conformation; D012319:RNA Polymerase II / Q000737:chemistry* / Q000502:physiology*; D035581:Transcription Factor TFIIB; D014157:Transcription Factors / Q000502:physiology; D019473:Transcription Factors, TFII / Q000502:physiology*; D014158:Transcription, Genetic
chemical_list: D035581:Transcription Factor TFIIB; D014157:Transcription Factors; D019473:Transcription Factors, TFII; D012319:RNA Polymerase II