title: Effect of Fas and Fas ligand deficiency in resistance of C57BL/6 mice to HSV-1 keratitis and chorioretinitis.
abstract: OBJECTIVE
  To investigate the effect of Fas and Fas ligand (FasL) deficiency on the development of herpes stromal keratitis and on the von Szily model of herpes retinitis in C57BL/6 mice, which are ordinarily resistant to development of both of these herpetic diseases.
  
  
  METHODS
  Anterior chamber inoculation of the right eye of each mouse with various titers of HSV-1 (KOS strain) was performed. Both eyes of each mouse were enucleated on postinoculation day 15 and processed for histopathologic examination. HSV-1 was inoculated into one cornea of other mice, and the severity of stromal keratitis was scored.
  
  
  RESULTS
  Contralateral destructive chorioretinitis developed in susceptible Balb/cByj mice (19/23); ipsilateral chorioretinitis did not occur (0/23). Stromal keratitis developed in susceptible C.AL-20 mice (15/16). None of the C57BL/6 (0/10 for keratitis or 0/20 for retinitis) developed inflammation. Neither did B6.SMN.C3H.gld (FasL deficient; 0/12 or 0/28) or B6.MRL.lpr (Fas deficient; 0/11 or 0/34) mice (keratitis or contralateral chorioretinitis). Minimal scattering of inflammatory cells in the contralateral retina but not destructive chorioretinitis was observed in two C57BL/6, three B6.SMN.C3H.gld, and five B6.MRL.lpr mice. Few inflammatory cells were also found in the ipsilateral vitreous and vitreoretinal interface (but not destructive chorioretinitis) of all C57BL/6, two gld, and three lpr mice.
  
  
  CONCLUSIONS
  Immune dysregulation secondary to deficiency in Fas or FasL system does not influence the resistance of the C57BL/6 mice to develop herpes simplex keratitis or destructive herpes simplex chorioretinitis.
authors.affiliation: Ocular Immunology and Uveitis Service, Massachusetts Eye and Ear Infirmary, Boston, Massachusetts 02114, USA.
authors.forename: Q D
authors.initials: QD
authors.lastname: Nguyen
authors.forename: H S
authors.initials: HS
authors.lastname: Uy
authors.forename: A
authors.initials: A
authors.lastname: Merchant
authors.forename: C A
authors.initials: CA
authors.lastname: Medina
authors.forename: S
authors.initials: S
authors.lastname: Baltatzis
authors.forename: T
authors.initials: T
authors.lastname: Zhao
authors.forename: Z S
authors.initials: ZS
authors.lastname: Zhao
authors.forename: H
authors.initials: H
authors.lastname: Cantor
authors.forename: C S
authors.initials: CS
authors.lastname: Foster
mesh_terms: D000818:Animals; D000867:Anterior Chamber / Q000821:virology; D002825:Chorioretinitis / Q000473:pathology / Q000517:prevention & control / Q000821:virology*; D003319:Corneal Stroma / Q000821:virology; D004198:Disease Susceptibility; D053222:Fas Ligand Protein; D005260:Female; D018259:Herpesvirus 1, Human / Q000502:physiology*; D016849:Keratitis, Herpetic / Q000473:pathology / Q000517:prevention & control / Q000821:virology*; D008297:Male; D008562:Membrane Glycoproteins / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D019463:Mice, Inbred MRL lpr; D019014:fas Receptor / Q000502:physiology*
chemical_list: C505661:FASLG protein, human; D053222:Fas Ligand Protein; C505658:Fasl protein, mouse; D008562:Membrane Glycoproteins; D019014:fas Receptor