Oxidative stress status during exposure to propofol, sevoflurane and desflurane.
We evaluated the circulating and lung oxidative status during general anesthesia established with propofol, sevoflurane, or desflurane in mechanically ventilated swine. Blood samples and bronchoalveolar lavage fluid (BAL) specimens were respectively performed via an internal jugular vein catheter and a nonbronchoscopic BAL for baseline oxidative activity measurements: malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione peroxidase (GPX). A 4-h general anesthesia was then performed in the three groups of 10 swine: the Propofol group received 8 mg x kg(-1) x h(-1) of IV propofol as the sole anesthetic; the Desflurane group received 1.0 minimum alveolar concentration of desflurane; and the Sevoflurane group received 1.0 minimum alveolar concentration of sevoflurane. We observed significantly larger levels of MDA in plasma and BAL during desflurane exposure than with the other anesthetics. We also observed smaller concentrations of circulating GPX and alveolar GPX. We found a significant decrease for MDA measurements in the plasma and the pulmonary lavage during propofol anesthesia. We also found larger values of GPX measurements in the serum and the pulmonary lavage. No significant changes were observed when animals were exposed to sevoflurane. No significant changes were found for circulating concentrations of SOD during exposure to all anesthetics. In this mechanically ventilated swine model, desflurane seemed to induce a local and systemic oxidative stress, whereas propofol and sevoflurane were more likely to have antioxidant properties.


CONCLUSIONS
Superoxide is an unavoidable byproduct of oxygen metabolism that occurs in various inflammatory reactions. Inhalation of volatile anesthetics under mechanical ventilation induces an inflammatory response. We evaluated the bronchoalveolar and systemic oxidative stress in swine during exposure to propofol and newer volatile anesthetics. Desflurane induces more lipid peroxidation than do the other anesthetics.
Allaouchiche
B
B
Department of Anesthesiology and Intensive Care, EA 18/96, Hotel-Dieu Hospital, Lyon, France, and Department of Biochemistry, Edouard Herriot Hospital, Lyon, France. allaouch@univ-lyon1.fr
Debon
R
R
Goudable
J
J
Chassard
D
D
Duflo
F
F
D018685:Anesthetics, Inhalation / Q000494:pharmacology*; D018686:Anesthetics, Intravenous / Q000494:pharmacology*; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry*; D000077335:Desflurane; D005979:Glutathione Peroxidase / Q000378:metabolism; D007530:Isoflurane / Q000031:analogs & derivatives / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects; D008315:Malondialdehyde / Q000097:blood; D008738:Methyl Ethers / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D015742:Propofol / Q000494:pharmacology*; D011650:Pulmonary Alveoli / Q000187:drug effects / Q000378:metabolism; D000077149:Sevoflurane; D013482:Superoxide Dismutase / Q000097:blood; D013552:Swine
D018685:Anesthetics, Inhalation; D018686:Anesthetics, Intravenous; D000975:Antioxidants; D008738:Methyl Ethers; D000077149:Sevoflurane; D008315:Malondialdehyde; D000077335:Desflurane; D007530:Isoflurane; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D015742:Propofol