Chitinase in cucumber xylem sap.
A chitinase activity was detected in fractions of xylem sap collected from the cut surface of cucumber stems. A 28-kDa acidic protein was purified from the active fractions and its N-terminal amino acid sequence was found to be identical to that of a chitinase gene. Cucumber roots produce and secrete an acidic chitinase, one of the PR proteins, into xylem sap and deliver it to aboveground organs.
Masuda
S
S
Noda Institute for Scientific Research, Chiba, Japan. susumu.masuda@nifty.ne.jp
Kamada
H
H
Satoh
S
S
D000595:Amino Acid Sequence; D002688:Chitinases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D002852:Chromatography, Ion Exchange; D018553:Cucumis sativus / Q000201:enzymology* / Q000235:genetics; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification; D018517:Plant Roots / Q000201:enzymology
D010455:Peptides; D002688:Chitinases