Rapid detection of methicillin resistance in coagulase-negative staphylococci by a penicillin-binding protein 2a-specific latex agglutination test.
The detection of PBP 2a by the MRSA-Screen latex agglutination test with 201 clinical coagulase-negative staphylococci had an initial sensitivity of 98% and a high degree of specificity for Staphylococcus epidermidis strains compared to PCR for mecA. Determination of oxacillin MICs evaluated according to the new breakpoint (0.5 microg/ml) of the National Committee for Clinical Laboratory Standards exhibited an extremely low specificity for this population.
Horstkotte
M A
MA
Institut für Medizinische Mikrobiologie und Immunologie, Universitätsklinikum Hamburg-Eppendorf, D-20246 Hamburg, Germany.
Knobloch
J K
JK
Rohde
H
H
Mack
D
D
D001426:Bacterial Proteins; D002352:Carrier Proteins / Q000378:metabolism*; D003030:Coagulase / Q000378:metabolism*; D006602:Hexosyltransferases; D006801:Humans; D007841:Latex Fixation Tests / Q000379:methods*; D016106:Methicillin Resistance / Q000235:genetics; D008826:Microbial Sensitivity Tests / Q000379:methods; D002267:Muramoylpentapeptide Carboxypeptidase / Q000378:metabolism*; D010068:Oxacillin / Q000494:pharmacology; D046915:Penicillin-Binding Proteins; D010406:Penicillins / Q000494:pharmacology; D010458:Peptidyl Transferases; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D013210:Staphylococcus / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics; D013997:Time Factors
D001426:Bacterial Proteins; D002352:Carrier Proteins; D003030:Coagulase; D046915:Penicillin-Binding Proteins; D010406:Penicillins; D010458:Peptidyl Transferases; D006602:Hexosyltransferases; D002267:Muramoylpentapeptide Carboxypeptidase; D010068:Oxacillin