title: Identification of DNA sequences from a second pathogenicity island of uropathogenic Escherichia coli CFT073: probes specific for uropathogenic populations.
abstract: Uropathogenic Escherichia coli is the leading cause of urinary tract infection and hospital visits in North America. Cystitis and acute pyelonephritis, infection of the bladder and kidney, respectively, are the two most common syndromes encountered in patients with urinary tract infection. We sequenced and annotated 71,684 bases of a previously unidentified pathogenicity-associated island (PAI) from E. coli strain CFT073. This PAI contained 89 open-reading frames encoding a pap operon, iron-regulated genes, mobile genetic elements, and a large proportion of unknown or unidentified open-reading frames. Dot blot analysis with 11 DNA sequences from this PAI demonstrated that 7 sequences were more prevalent among uropathogens: 2 probes were more prevalent among cystitis and pyelonephritis isolates, 2 among pyelonephritis isolates only, and 3 among cystitis isolates only than among fecal isolates. These data suggest that groups of uropathogens have genetic differences that may be responsible for the different clinical outcomes.
authors.affiliation: Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, MD 21201, USA.
authors.forename: D A
authors.initials: DA
authors.lastname: Rasko
authors.forename: J A
authors.initials: JA
authors.lastname: Phillips
authors.forename: X
authors.initials: X
authors.lastname: Li
authors.forename: H L
authors.initials: HL
authors.lastname: Mobley
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D004798:Enzymes / Q000235:genetics; D004926:Escherichia coli / Q000145:classification* / Q000235:genetics / Q000472:pathogenicity; D004927:Escherichia coli Infections / Q000382:microbiology; D006801:Humans; D016366:Open Reading Frames; D014552:Urinary Tract Infections / Q000382:microbiology
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004798:Enzymes