Binding and partial denaturing of G-quartet DNA by Cdc13p of Saccharomyces cerevisiae.
The protein Cdc13p binds telomeres in vivo and is essential for the maintenance of the telomeres of Saccharomyces cerevisiae. In addition, Cdc13p is known to bind single-stranded TG(1-3) DNA in vitro. Here we have shown that Cdc13p also binds DNA quadruplex, G-quartet, formed by TG(1-3) DNA. Moreover, the binding of Cdc13p causes a partial denaturing of the G-quartet DNA. Formation of DNA quadruplexes may involve the intermolecular association of TG(1-3) DNA and inhibit the extension of telomeres by telomerase. Thus, our finding suggests that Cdc13p may disrupt telomere association and facilitate telomere replication.
Lin
Y C
YC
Institute of Biopharmaceutical Science, National Yang-Ming University, Shih-Pai, 112, Taipei, Taiwan, Republic of China.
Shih
J W
JW
Hsu
C L
CL
Lin
J J
JJ
D000477:Alkylating Agents / Q000494:pharmacology; D000818:Animals; D016367:Baculoviridae; D002460:Cell Line; D019926:Cyclin B / Q000378:metabolism*; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism; D007313:Insecta; D009153:Mutagens / Q000494:pharmacology; D009690:Nucleic Acid Conformation; D010957:Plasmids / Q000378:metabolism; D011485:Protein Binding; D011489:Protein Denaturation; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*; D013463:Sulfuric Acid Esters / Q000378:metabolism; D016615:Telomere / Q000378:metabolism
D000477:Alkylating Agents; D019926:Cyclin B; D009153:Mutagens; D013463:Sulfuric Acid Esters; D004247:DNA; C007482:dimethyl sulfate