Elevated levels of high-mobility-group chromosomal proteins, HMGA1, in murine skin carcinoma.
The high-mobility-group, HMGA1 (formerly HMGI(Y)) chromosomal proteins are known to be involved in gene regulation and their high expression is associated with neoplastic transformation of cells and metastatic tumor progression. Here, we present our results on the expression of HMGA1 in murine skin carcinoma as detected by acid-urea electrophoresis, reverse-phase high-performance liquid chromatography and Western blot. The enhanced expression of HMGA1 proteins directly correlates with the extent of cellular atypia and neoplastic changes noticed in the histopathology of tumor and suggest a potential use of these proteins as marker for determining the grade of skin tumor.
Rajeswari
M R
MR
Department of Biochemistry, All India Institute of Medical Sciences, New Delhi 110029, India. mrraji@hotmail.com
Singh
D
D
Jain
A
A
Ray
R
R
D019342:Acetic Acid / Q000737:chemistry; D000818:Animals; D001564:Benzo(a)pyrene; D014408:Biomarkers, Tumor / Q000378:metabolism; D015153:Blotting, Western; D002277:Carcinoma / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D025741:HMGA1a Protein / Q000096:biosynthesis*; D051379:Mice; D012878:Skin Neoplasms / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D014508:Urea / Q000737:chemistry
D014408:Biomarkers, Tumor; D025741:HMGA1a Protein; D001564:Benzo(a)pyrene; D014508:Urea; D019342:Acetic Acid