Detection of bovine herpesvirus-1 in peripheral blood mononuclear cells eight months postinfection.
Peripheral blood mononuclear cells (PBMCs) from 5 calves (3 controls and 2 vaccinates) used in a bovine herpesvirus 1 (BHV-1) vaccine study with a BHV-1 Cooper strain challenge were collected 6 months after challenge. The PBMCs from the control animals were positive by immunofluorescence for the BHV-1 glycoprotein D (gD) while the vaccinates were negative. The PBMC samples from 4 of the 5 animals were examined for BHV-1 DNA by polymerase chain reaction (PCR) and for gD immunofluorescence at 8 months after challenge. The BHV-1 DNA and viral antigen were detected in PBMC samples at 8 months postinfection, but no virus was isolated.
Wang
P
P
Department of Veterinary Science, South Dakota State University, Brookings 57007, USA.
Hurley
D J
DJ
Braun
L J
LJ
Chase
C C
CC
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000235:genetics; D004279:DNA, Viral / Q000032:analysis*; D005455:Fluorescent Antibody Technique / Q000662:veterinary; D006566:Herpesviridae Infections / Q000175:diagnosis / Q000235:genetics / Q000662:veterinary*; D007242:Herpesvirus 1, Bovine / Q000235:genetics* / Q000302:isolation & purification; D022283:Herpesvirus Vaccines; D007963:Leukocytes, Mononuclear / Q000821:virology; D016133:Polymerase Chain Reaction / Q000662:veterinary; D013048:Specimen Handling; D014611:Vaccination / Q000662:veterinary
D004279:DNA, Viral; D022283:Herpesvirus Vaccines