title: Binding and partial denaturing of G-quartet DNA by Cdc13p of Saccharomyces cerevisiae.
abstract: The protein Cdc13p binds telomeres in vivo and is essential for the maintenance of the telomeres of Saccharomyces cerevisiae. In addition, Cdc13p is known to bind single-stranded TG(1-3) DNA in vitro. Here we have shown that Cdc13p also binds DNA quadruplex, G-quartet, formed by TG(1-3) DNA. Moreover, the binding of Cdc13p causes a partial denaturing of the G-quartet DNA. Formation of DNA quadruplexes may involve the intermolecular association of TG(1-3) DNA and inhibit the extension of telomeres by telomerase. Thus, our finding suggests that Cdc13p may disrupt telomere association and facilitate telomere replication.
authors.affiliation: Institute of Biopharmaceutical Science, National Yang-Ming University, Shih-Pai, 112, Taipei, Taiwan, Republic of China.
authors.forename: Y C
authors.initials: YC
authors.lastname: Lin
authors.forename: J W
authors.initials: JW
authors.lastname: Shih
authors.forename: C L
authors.initials: CL
authors.lastname: Hsu
authors.forename: J J
authors.initials: JJ
authors.lastname: Lin
mesh_terms: D000477:Alkylating Agents / Q000494:pharmacology; D000818:Animals; D016367:Baculoviridae; D002460:Cell Line; D019926:Cyclin B / Q000378:metabolism*; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism; D007313:Insecta; D009153:Mutagens / Q000494:pharmacology; D009690:Nucleic Acid Conformation; D010957:Plasmids / Q000378:metabolism; D011485:Protein Binding; D011489:Protein Denaturation; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*; D013463:Sulfuric Acid Esters / Q000378:metabolism; D016615:Telomere / Q000378:metabolism
chemical_list: D000477:Alkylating Agents; D019926:Cyclin B; D009153:Mutagens; D013463:Sulfuric Acid Esters; D004247:DNA; C007482:dimethyl sulfate