Identification and determination of glucuronides and their aglycones in Erigeron breviscapus by liquid chromatography-tandem mass spectrometry.
A novel, speedy and reliable LC-MS-MS method for the search, identification and quantitation of O-glycosides and their aglycones from plant resources was established by analyzing the extract of Erigeron breviscapus. The extract was directly infused to a triple-quadrupole MS-MS and major glucuronides in the extract were screened out with high confidence by a neutral loss scan for the loss of a gluconic acid. The identity of these glucuronides and their aglycones was further confirmed with LC-MS-MS. In addition to scutellarin, apigenin 7-O-glucuronide, quercetin-3-O-glucuronide and their aglycones that were previously reported by others, we also confirmed by LC-MS-MS that remarkable amount of baicalin, an isomer of apigenin 7-O-glucuronide, presented in the extract of this plant, which had not been reported before. A satisfying quantitation of three glucuronides was also made by LC-MS-MS.
Qu
J
J
Department of Chemistry, School of Life Science and Engineering, Tsinghua University, Beijing, PR China.
Wang
Y
Y
Luo
G
G
Wu
Z
Z
D019659:Asteraceae / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods*; D005419:Flavonoids / Q000302:isolation & purification; D020719:Glucuronides / Q000302:isolation & purification*; D013058:Mass Spectrometry / Q000379:methods*; D012680:Sensitivity and Specificity
D005419:Flavonoids; D020719:Glucuronides; C038044:baicalin