Expression pattern of DMAP-85 during Drosophila embryonic development.
Microtubule-associated proteins (MAPs) play major regulatory roles on the organization and integrity of the cytoskeletal network. Previously, we identified DMAP-85, a Drosophila MAP that promotes tubulin polymerization in vitro. In this work, we examine the distribution of DMAP-85 and its association pattern with microtubules at embryonic stages. Immunoblots revealed that DMAP-85 was present throughout embryogenesis, but it was most abundant in stages 6-9. Immunofluorescence studies showed that DMAP-85 was associated with sub-populations of stable microtubules during embryo cellularization, and after gastrulation with interphase microtubule arrays. At late embryonic stages, it was preferentially found in the ventral nerve cord, co-localizing with axonal microtubules. These observations are in agreement with previous reports on DMAP-85 functions, suggesting that DMAP-85 might be required for the stabilization and organization of cytoplasmic microtubules during embryonic development.
Cambiazo
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Laboratorio de Biología Celular y Molecular, Facultad de Ciencias, Universidad de Chile, Las Palmeras 3425, Santiago, Chile. vcambiaz@uec.inta.uchile.cl
González
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M
Maccioni
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RB
D000818:Animals; D004330:Drosophila / Q000196:embryology* / Q000235:genetics* / Q000378:metabolism; D029721:Drosophila Proteins / Q000235:genetics* / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D017344:Genes, Insect; D007150:Immunohistochemistry; D008856:Microscopy, Fluorescence; D008869:Microtubule-Associated Proteins / Q000235:genetics* / Q000378:metabolism; D009420:Nervous System / Q000196:embryology / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
D029721:Drosophila Proteins; D008869:Microtubule-Associated Proteins; D012333:RNA, Messenger