'Empty' minicircles and petB/atpA and psbD/psbE (cytb559 alpha) genes in tandem in Amphidinium carterae plastid DNA.
Amphidinium carterae minicircle chloroplast DNA was separated from total DNA by centrifugation through a sucrose/NaCl gradient. Sequences of minicircles with psbA and 23S rRNA contained a common region of 67 bp. Primers designed from this generated numerous polymerase chain reaction products of 1.5-2.6 kb. These contained psaA and psaB as one gene/circle, and petB/atpA and psbD/psbE as two genes/circle. 'Empty' minicircles of 1.7-2.5 kb containing no identifiable genes or parts of genes were more abundant than gene-containing circles. From 15 minicircles a minimum common region of 48 bp was identified, with little identity to that from other dinoflagellate minicircles.
Hiller
R G
RG
Department of Biology, Macquarie University, Macquarie, NSW 2109, Australia. rhiller@rna.bio.mq.edu.au
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003573:Cytochrome b Group / Q000235:genetics; D016054:DNA, Protozoan / Q000235:genetics*; D004141:Dinoflagellida / Q000235:genetics*; D017125:Genes, Protozoan; D008969:Molecular Sequence Data; D045322:Photosynthetic Reaction Center Complex Proteins / Q000235:genetics; D045332:Photosystem II Protein Complex; D010940:Plant Proteins / Q000235:genetics; D018087:Plastids / Q000235:genetics*
D003573:Cytochrome b Group; D016054:DNA, Protozoan; C104657:PetB protein, plant; D045322:Photosynthetic Reaction Center Complex Proteins; D045332:Photosystem II Protein Complex; D010940:Plant Proteins; C120632:PsbE protein, Amphidinium carterae; C019313:cytochrome b559