Inhibitory effect of losartan on laser-induced choroidal neovascularization in rats.
OBJECTIVE
To investigate the inhibitory effects of losartan, an angiotensin receptor antagonist, on angiogenesis in a rat model of laser-induced choroidal neovascularization.


METHODS
Experimental study. Fifteen Brown-Norway male rats received losartan (approximately 5 mg/kg/d) in drinking water, and 15 Brown-Norway male rats received unsupplemented drinking water 1 week before photocoagulation, and it was continued to the end of the study. Two weeks after intense laser photocoagulation, choroidal neovascularization was evaluated by fluorescein angiography and histopathologic evaluation.


RESULTS
The incidence of choroidal neovascularization formation was 99.5 +/-.2% (mean +/- standard deviation) in controls and 72.5 +/- 8.8% in losartan-treated rats (P <.01). Quantitative morphometric assessment revealed mean choroidal neovascularization lesion thickness of 54 and 44.8 microm, respectively, in controls and losartan-treated rats (P <.01).


CONCLUSIONS
Losartan seems to inhibit development of laser-induced choroidal neovascularization. Angiotensin receptor antagonists may be useful as prophylaxis against choroidal neovascularization associated with age-related macular degeneration.
Hikichi
T
T
Department of Ophthalmology, Asahikawa Medical College, 2-1 Midorigaoka-higashi, Asahikawa, 078-8510, Japan. hikichi@asahikawa-med.ac.jp
Mori
F
F
Takamiya
A
A
Sasaki
M
M
Horikawa
Y
Y
Takeda
M
M
Yoshida
A
A
D057911:Angiotensin Receptor Antagonists; D000818:Animals; D002829:Choroid / Q000601:surgery*; D020256:Choroidal Neovascularization / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*; D005451:Fluorescein Angiography; D017075:Laser Coagulation; D019808:Losartan / Q000627:therapeutic use*; D008297:Male; D023421:Models, Animal; D051381:Rats; D011914:Rats, Inbred BN; D044140:Receptor, Angiotensin, Type 1; D044139:Receptor, Angiotensin, Type 2
D057911:Angiotensin Receptor Antagonists; D044140:Receptor, Angiotensin, Type 1; D044139:Receptor, Angiotensin, Type 2; D019808:Losartan