Elevation of muscle and plasma 3-methylhistidine as a result of turkey coccidiosis.
To assess muscle breakdown during avian coccidiosis, the level of the non-metabolizable amino acid 3-methylhistidine (3MH) was determined in muscle and plasma from turkey poults that received an infection with a field isolate containing a mixture of Eimeria species. The effect of increased levels of parasitism was evaluated at 6 days postinoculation (DPI) in birds receiving 2.5 x 10(4), 1 x 10(5), or 2 x 10(5) oocysts each. The changes in 3MH levels during recovery from acute infection were assessed at 6-29 DPI in animals given 1.9 x 10(5) oocysts per bird. In some experiments, uninoculated birds given the same amount of feed as infected birds (pair fed) were used to determine the impact of feed deprivation on weight loss and 3MH levels. Infected birds had significantly elevated plasma and muscle 3MH at 6 DPI after a single dose of Eimeria oocysts. The plasma and muscle 3MH returned to control levels after 14 DPI. The 3MH levels increased with increased dose of oocysts. Plasma and muscle 3MH levels were well correlated, and an inverse curvilinear relationship between weight gain and plasma 3MH concentrations levels was observed. Plasma and muscle 3MH levels were significantly elevated in pair-fed birds, but 3MH levels in infected birds were increased by 30% over pair-fed birds. The results suggested that muscle breakdown, as assessed by plasma and muscle levels of 3MH, increased during the acute stage of Eimeria infection in turkey poults.
Fetterer
R H
RH
Parasite Biology and Epidemiology Laboratory, Livestock and Poultry Sciences Institute, Beltsville, MD 20705, USA.
Augustine
P C
PC
D000208:Acute Disease; D000821:Animal Feed; D000818:Animals; D003048:Coccidiosis / Q000097:blood / Q000378:metabolism / Q000662:veterinary*; D004539:Eimeria; D005260:Female; D005508:Food Deprivation; D008297:Male; D008762:Methylhistidines / Q000097:blood / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000378:metabolism*; D011201:Poultry Diseases / Q000097:blood / Q000378:metabolism* / Q000469:parasitology; D013997:Time Factors; D014422:Turkeys / Q000469:parasitology*; D015430:Weight Gain
D008762:Methylhistidines; C028118:3-methylhistidine