title: Association of 3-methyleneindolenine, a toxic metabolite of 3-methylindole, with acute interstitial pneumonia in feedlot cattle.
abstract: OBJECTIVE
  To compare concentrations of 3-methyleneindolenine (3MEIN) in lung tissues obtained from feedlot cattle that died as a result of acute interstitial pneumonia (AIP) and cattle that died as a result of other causes and to compare blood concentrations of 3MEIN in healthy feedlot cattle and feedlot cattle with AIP.
  
  
  METHODS
  Blood samples and lung tissues collected from 186 cattle housed in 14 feedlots in the western United States.
  
  
  METHODS
  Samples of lung tissues were collected during routine postmortem examination and submitted for histologic, microbiologic, and toxicologic examination. Blood samples were collected from cattle with clinical manifestations of AIP and healthy penmates. Histologic diagnoses were categorized as AIP, bronchopneumonia (BP), control samples, and other disorders. Concentrations of 3MEIN were determined in lung tissues and blood samples, using an ELISA.
  
  
  RESULTS
  Concentrations of 3MEIN in lung tissues were significantly greater in AIP and BP samples, compared with control samples. Absorbance per microgram of protein did not differ between BP and AIP samples. Blood concentrations of 3MEIN were significantly greater in cattle with AIP, compared with healthy cattle or cattle with BP. Odds of an animal with AIP being a heifer was 3.1 times greater than the odds of that animal being a steer.
  
  
  CONCLUSIONS
  Increased pulmonary production of 3MEIN may be an important etiologic factor in feedlot-associated AIP.
authors.affiliation: Department of Clinical Sciences, Colorado State University, Fort Collins 80523, USA.
authors.forename: G H
authors.initials: GH
authors.lastname: Loneragan
authors.forename: D H
authors.initials: DH
authors.lastname: Gould
authors.forename: G L
authors.initials: GL
authors.lastname: Mason
authors.forename: F B
authors.initials: FB
authors.lastname: Garry
authors.forename: G S
authors.initials: GS
authors.lastname: Yost
authors.forename: D L
authors.initials: DL
authors.lastname: Lanza
authors.forename: D G
authors.initials: DG
authors.lastname: Miles
authors.forename: B W
authors.initials: BW
authors.lastname: Hoffman
authors.forename: L J
authors.initials: LJ
authors.lastname: Mills
mesh_terms: D000818:Animals; D001996:Bronchopneumonia / Q000097:blood / Q000378:metabolism / Q000662:veterinary*; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D006651:Histocytochemistry / Q000662:veterinary; D007211:Indoles / Q000097:blood / Q000378:metabolism*; D008168:Lung / Q000378:metabolism*; D017563:Lung Diseases, Interstitial / Q000097:blood / Q000378:metabolism / Q000662:veterinary*; D008297:Male; D012737:Sex Factors
chemical_list: D007211:Indoles; C097745:3-methyleneindolenine