title: Cytokines involved in the immunosuppressor period in experimental fasciolosis in rats.
abstract: The aim of this study was to evaluate the kinetics of the cytokines interferon-gamma, interleukin-2, interleukin-10 and interleukin-4 produced by spleen mononuclear cells stimulated by Con A during an experimental infection in rats with Fasciola hepatica. The proliferative response to Con A of Spm cells from rats infected with F. hepatica was significantly decreased on day 7 post-infection (P<0.006) and simultaneously an increase of interferon-gamma, interleukin-10 and interleukin-4 production along with a decrease of interleukin-2 by spleen mononuclear cells were observed. Interleukin-4 and interleukin-10 were involved in ablating cellular proliferation in vitro, as the addition of neutralising antibodies to either cytokine reversed the proliferative block. The addition of exogenous recombinant interleukin-2 also restored the proliferative response by spleen mononuclear cells obtained 7 days after infection from infected rats. At the same time, we found an increase in interleukin-10 production by peritoneal cells (in close contact with the flukes) and decreased nitric oxide levels. In addition, histological studies on the liver on day 7 after infection showed the presence of parasite inside migratory tunnels in the parenchyma, and polymorphonuclear leukocytes, predominantly eosinophils, around the parasite. The transient suppression in proliferative response mediated by cytokines interleukin-4 and interleukin-10 in the spleen, and diminution of nitric oxide production in the peritoneum could be mechanisms to evade the protective immune response during the first stages of liver penetration by the parasite.
authors.affiliation: Parasitología, Departamento de Bioquímica Clínica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Ciudad Universitaria, 5000 Cordoba, Argentina.
authors.forename: L
authors.initials: L
authors.lastname: Cervi
authors.forename: H
authors.initials: H
authors.lastname: Cejas
authors.forename: D T
authors.initials: DT
authors.lastname: Masih
mesh_terms: D000818:Animals; D003208:Concanavalin A / Q000276:immunology; D016207:Cytokines / Q000096:biosynthesis* / Q000097:blood; D005210:Fasciola hepatica / Q000276:immunology*; D005211:Fascioliasis / Q000097:blood / Q000276:immunology*; D005260:Female; D006651:Histocytochemistry; D007371:Interferon-gamma / Q000096:biosynthesis / Q000097:blood; D007378:Interleukins / Q000096:biosynthesis / Q000097:blood; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000378:metabolism; D008099:Liver / Q000469:parasitology / Q000473:pathology; D017737:Macrophages, Peritoneal / Q000276:immunology; D009569:Nitric Oxide / Q000032:analysis / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D013154:Spleen / Q000166:cytology / Q000276:immunology / Q000378:metabolism
chemical_list: D016207:Cytokines; D007378:Interleukins; D003208:Concanavalin A; D009569:Nitric Oxide; D007371:Interferon-gamma