Effects of methanol on expression of an anticoagulant hirudin in recombinant Hansenula polymorpha.
A series of batch, fed-batch, and continuous cultures was carried out to analyze the effects of methanol on the fermentation characteristics of recombinant Hansenula polymorpha for the production of hirudin, an anticoagulant. Hirudin expression efficiencies were greatly influenced by the methanol concentrations in continuous and fed-batch culture modes. At a steady state of continuous culture, an optimum methanol concentration of 1.7 g l(-1) was determined at a dilution rate of 0.18 h(-1) with 1.8 mg l(-1) h(-1) hirudin productivity.
Kim
M D
MD
Department of Food Science and Technology and Research Center for New Biomaterials in Agriculture, Seoul National University, Suwon 441-744, Korea.
Kang
H A
HA
Rhee
S K
SK
Seo
J H
JH
D000429:Alcohol Oxidoreductases / Q000378:metabolism; D003470:Culture Media; D006629:Hirudins / Q000235:genetics* / Q000378:metabolism*; D000432:Methanol / Q000494:pharmacology*; D010843:Pichia / Q000187:drug effects* / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D011995:Recombination, Genetic
D003470:Culture Media; D006629:Hirudins; D000429:Alcohol Oxidoreductases; C029293:methanol oxidase; D000432:Methanol