title: Heat-induced formation of a specific binding site for self-assembled Congo Red in the V domain of immunoglobulin L chain lambda.
abstract: Moderate heating (40-50 degrees C) of immunoglobulins makes them accessible for binding with Congo Red and some related highly associated dyes. The binding is specific and involves supramolecular dye ligands presenting ribbon-like micellar bodies. The L chain lambda dimer, which upon heating disclosed the same binding requirement with respect to supramolecular dye ligands, was used in this work to identify the site of their attachment. Two clearly defined dye-protein (L lambda chain) complexes arise upon heating, here called complex I and complex II. The first is formed at low temperatures (up to 40-45 degrees C) and hence by a still native protein, while the formation of the second one is associated with domain melting above 55 degrees C. They contain 4 and 8 dye molecules bound per L chain monomer, respectively. Complex I also forms efficiently at high dye concentration even at ambient temperature. Complex I and its formation was the object of the present studies. Three structural events that could make the protein accessible to penetration by the large dye ligand were considered to occur in L chains upon heating: local polypeptide chain destabilization, VL-VL domain incoherence, and protein melting. Of these three possibilities, local low-energy structural alteration was found to correlate best with the formation of complex I. It was identified as decreased packing stability of the N-terminal polypeptide chain fragment, which as a result made the V domain accessible for dye penetration. The 19-amino acid N-terminal fragment becomes susceptible to proteolytic cleavage after being replaced by the dye at its packing locus. Its splitting from the dye-protein complex was proved by amino acid sequence analysis. The emptied packing locus, which becomes the site that holds the dye, is bordered by strands of amino acids numbered 74-80 and 105-110, as shown by model analysis. The character of the temperature-induced local polypeptide chain destabilization and its possible role in intramolecular antibody signaling is discussed.
authors.affiliation: Institute of Medical Biochemistry, Collegium Medicum-Jagiellonian University, Kopernika St. 7, 31-034 Kraków, Poland. mbpiekar@cyf-kr-edu.pl
authors.forename: B
authors.initials: B
authors.lastname: Piekarska
authors.forename: L
authors.initials: L
authors.lastname: Konieczny
authors.forename: J
authors.initials: J
authors.lastname: Rybarska
authors.forename: B
authors.initials: B
authors.lastname: Stopa
authors.forename: G
authors.initials: G
authors.lastname: Zemanek
authors.forename: E
authors.initials: E
authors.lastname: Szneler
authors.forename: M
authors.initials: M
authors.lastname: Król
authors.forename: M
authors.initials: M
authors.lastname: Nowak
authors.forename: I
authors.initials: I
authors.lastname: Roterman
mesh_terms: D000595:Amino Acid Sequence; D001666:Binding Sites, Antibody; D001704:Biopolymers / Q000737:chemistry; D004396:Coloring Agents / Q000737:chemistry; D003224:Congo Red / Q000737:chemistry; D006358:Hot Temperature; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000737:chemistry* / Q000235:genetics; D007146:Immunoglobulin lambda-Chains / Q000737:chemistry* / Q000235:genetics; D066298:In Vitro Techniques; D046911:Macromolecular Substances; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D008969:Molecular Sequence Data
chemical_list: D001704:Biopolymers; D004396:Coloring Agents; D007135:Immunoglobulin Variable Region; D007146:Immunoglobulin lambda-Chains; D046911:Macromolecular Substances; D003224:Congo Red