title: Characterization of an unknown component in Noscapine using liquid chromatography-mass spectrometry and proton nuclear magnetic resonance spectroscopy.
abstract: Analytical and semipreparative LC methods were used to quantitate and isolate an unknown component (Impurity A) found in samples of bulk Noscapine. This component was also examined by LC-ESI-MS and 1H-NMR. It was concluded that the structure of Impurity A only differed from Noscapine in that it possessed a hydroxyl group at position 21 of the isobenzofuranone moiety.
authors.affiliation: Tyco Healthcare/Mallinckrodt, St. Louis, MO, USA. mike.matchett@mkg.com
authors.forename: M W
authors.initials: MW
authors.lastname: Matchett
authors.forename: D W
authors.initials: DW
authors.lastname: Berberich
authors.forename: J E
authors.initials: JE
authors.lastname: Johnson
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D009665:Noscapine / Q000737:chemistry*; D011522:Protons
chemical_list: D011522:Protons; D009665:Noscapine