title: An examination of the potential role of spider digestive proteases as a causative factor in spider bite necrosis.
abstract: Tissue necrosis following spider bites is a widespread problem. In the continental United States, the brown recluse (Loxosceles reclusa), hobo spider (Tegenaria agrestis), garden spider (Argiope aurantia) and Chiracanthium species, among others, reportedly cause such lesions. The exact mechanism producing such lesions is controversial. There is evidence for both venom sphingomyelinase and spider digestive collagenases. We have examined the role of spider digestive proteases in spider bite necrosis. The digestive fluid of A. aurantia was assayed for its ability to cleave a variety of connective tissue proteins, including collagen. Having confirmed that the fluid has collagenases, the digestive fluid was injected into the skin of rabbits to observe whether it would cause necrotic lesions. It did not. The data do not support the suggestions that spider digestive collagenases have a primary role in spider bite necrosis.
authors.affiliation: Department of Zoology, University of New Hampshire, Durham, NH 03824, USA. foradori@cisunix.unh.edu
authors.forename: M J
authors.initials: MJ
authors.lastname: Foradori
authors.forename: L M
authors.initials: LM
authors.lastname: Keil
authors.forename: R E
authors.initials: RE
authors.lastname: Wells
authors.forename: M
authors.initials: M
authors.lastname: Diem
authors.forename: E K
authors.initials: EK
authors.lastname: Tillinghast
mesh_terms: D000818:Animals; D003094:Collagen / Q000378:metabolism; D017364:Collagenases / Q000378:metabolism; D003238:Connective Tissue / Q000201:enzymology; D004549:Elastin / Q000378:metabolism; D010450:Endopeptidases / Q000009:adverse effects* / Q000737:chemistry / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D005260:Female; D005337:Fibrin / Q000378:metabolism; D006458:Hemolymph / Q000378:metabolism; D008970:Molecular Weight; D009336:Necrosis; D011817:Rabbits; D012867:Skin / Q000473:pathology*; D001098:Spider Bites / Q000473:pathology*; D013111:Spider Venoms / Q000009:adverse effects*; D013112:Spiders / Q000201:enzymology*
chemical_list: D016326:Extracellular Matrix Proteins; D013111:Spider Venoms; D005337:Fibrin; D003094:Collagen; D004549:Elastin; D010450:Endopeptidases; D017364:Collagenases