The urokinase plasminogen activator receptor-associated protein/endo180 is coexpressed with its interaction partners urokinase plasminogen activator receptor and matrix metalloprotease-13 during osteogenesis.
The urokinase plasminogen activator receptor-associated protein/Endo180 (uPARAP/Endo180) is a newly discovered member of the macrophage mannose receptor family that was reported to interact with ligand-bound urokinase plasminogen activator receptor (uPAR), matrix metalloprotease-13 (MMP-13), and collagen V on the cell surface. We have determined the sites of expression of this novel receptor during murine postimplantation development. uPARAP/Endo180 was expressed in all tissues undergoing primary ossification, including the developing bones of the viscerocranium and calvarium that ossify intramembranously, and developing long bones undergoing endochondral ossification. uPARAP/Endo180 mRNA was expressed by both immature osteoblasts and by mature osteocalcin-producing osteoblasts-osteocytes, and was coexpressed with MMP-13. Interestingly, osteoblasts also expressed uPAR. Besides bone-forming tissues, uPARAP/Endo180 expression was detected only in a mesenchymal condensation of the midbrain and in the developing lungs. The data suggest a function of this novel protease receptor in bone development, possibly mediated through its interactions with uPAR, MMP-13, or collagen V.
Engelholm
L H
LH
Oral and Pharyngeal Cancer Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland, USA.
Nielsen
B S
BS
Netzel-Arnett
S
S
Solberg
H
H
Chen
X D
XD
Lopez Garcia
J M
JM
Lopez-Otin
C
C
Young
M F
MF
Birkedal-Hansen
H
H
Danø
K
K
Lund
L R
LR
Behrendt
N
N
Bugge
T H
TH
D000818:Animals; D001842:Bone and Bones / Q000196:embryology / Q000502:physiology*; D017364:Collagenases / Q000096:biosynthesis*; D005314:Embryonic and Fetal Development; D005260:Female; D007150:Immunohistochemistry; D053509:Matrix Metalloproteinase 13; D051379:Mice; D010012:Osteogenesis / Q000502:physiology; D011247:Pregnancy; D011956:Receptors, Cell Surface / Q000096:biosynthesis*; D011975:Receptors, Mitogen / Q000096:biosynthesis*; D055293:Receptors, Urokinase Plasminogen Activator
C408294:Endo180; C524899:Plaur protein, mouse; D011956:Receptors, Cell Surface; D011975:Receptors, Mitogen; D055293:Receptors, Urokinase Plasminogen Activator; D017364:Collagenases; D053509:Matrix Metalloproteinase 13; C507081:Mmp13 protein, mouse