Cell-type-specific expression of the TFIID component TAF(II)135 in the nervous system.
A number of nervous system-specific enhancers and silencers have been isolated and characterized. However, the detailed mechanism of cell- and tissue-specific regulation of transcription is to a large extent unknown and the role of the basal transcriptional complex components in these processes is mostly unclear. Here we demonstrate that mRNA levels of TATA binding protein-associated factor TAF(II)135 are upregulated in neuronal cells during development. In addition, induction of neuronal differentiation of teratocarcinoma PCC7 cells results in dramatic induction of TAF(II)135 mRNA levels and activation of a variety of promoters. The stimulation of promoter activity in differentiating cells is mimicked by the overexpression of TAF(II)135. As neuronal differentiation requires changes in the general pattern of transcriptional activity, we suggest that increased levels of TAF(II)135 facilitate the induction of a large number of neuronal genes.
Metsis
M
M
Laboratory of Molecular Neurobiology, Karolinska Institute, Stockholm, 17177, Sweden. madis@cajal.mbb.ki.se
Brunkhorst
A
A
Neuman
T
T
D000818:Animals; D015152:Blotting, Northern; D001921:Brain / Q000378:metabolism; D002454:Cell Differentiation; D002478:Cells, Cultured; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D051379:Mice; D009420:Nervous System / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D012313:RNA / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D035381:TATA-Binding Protein Associated Factors; D013997:Time Factors; D015534:Trans-Activators / Q000096:biosynthesis* / Q000235:genetics*; D035362:Transcription Factor TFIID; D019473:Transcription Factors, TFII / Q000096:biosynthesis* / Q000235:genetics*; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
D018076:DNA, Complementary; D012333:RNA, Messenger; C523528:TAF4 protein, mouse; D035381:TATA-Binding Protein Associated Factors; D015534:Trans-Activators; D035362:Transcription Factor TFIID; D019473:Transcription Factors, TFII; D012313:RNA