Analysis of haploid development based on expression patterns of developmental genes in the medaka Oryzias latipes.
The abnormalities of haploid medaka embryos were characterized by comparative analysis of histologic sections and expression patterns of some developmental marker genes between haploids and diploids to clarify whether medaka haploids are useful for identifying mutants. During gastrulation, an obvious defect was first observed as a delay of epiboly and involution. This delay was shown to be caused not by the perturbation of mesoderm induction, but by widespread cell death and disorganization of cell arrangement in the blastoderm. This disorganization of cell arrangement was also detected in various organs, such as the brain, somite and notochord, at a late developmental stage. Ten days after fertilization, a small head and a short body axis were formed; these changes were also observed in haploid embryos in other species, but their cause is unknown. Based on the expression patterns of HNF3beta and goosecoid, it was demonstrated that a short and impotent prechordal plate induced near the marginal zone in haploid embryos was responsible for this defect. However, in these experiments it was also demonstrated that many major organs in haploids, such as the somite and notochord, differentiated incompletely but were present. Therefore, it was concluded that haploid screening is suitable for identifying mutations revealed by an obvious phenotype, such as dorsoventral polarity.
Araki
K
K
Cell Engineering Section, Inland Station, National Research Institute of Aquaculture, Tamaki, Watarai, Mie 519-0423, Japan. arakin@affrc.go.jp
Okamoto
H
H
Graveson
A C
AC
Nakayama
I
I
Nagoya
H
H
D000595:Amino Acid Sequence; D000818:Animals; D004268:DNA-Binding Proteins / Q000096:biosynthesis; D005326:Fetal Proteins; D005775:Gastrula / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D005819:Genetic Markers; D006238:Haploidy; D053823:Hedgehog Proteins; D051542:Hepatocyte Nuclear Factor 3-beta; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009154:Mutation; D009687:Nuclear Proteins / Q000096:biosynthesis; D009693:Nucleic Acid Hybridization; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D009990:Oryzias; D010641:Phenotype; D017386:Sequence Homology, Amino Acid; D020825:T-Box Domain Proteins / Q000096:biosynthesis / Q000235:genetics; D013997:Time Factors; D015534:Trans-Activators / Q000096:biosynthesis; D014157:Transcription Factors; D000098908:Brachyury Protein
D004268:DNA-Binding Proteins; D005326:Fetal Proteins; D005819:Genetic Markers; D053823:Hedgehog Proteins; D051542:Hepatocyte Nuclear Factor 3-beta; D009687:Nuclear Proteins; D016376:Oligonucleotides, Antisense; D020825:T-Box Domain Proteins; D015534:Trans-Activators; D014157:Transcription Factors; D000098908:Brachyury Protein