title: Selective attraction of naive and memory B cells by dendritic cells.
abstract: In this study, we investigate whether dendritic cells (DC), known to interact directly with T and B cells, might also contribute to the recruitment of B cells through the production of chemotactic factors. We found that B cells responded to several chemokines (CXCL12, CCL19, CCL20, and CCL21), which can be produced by DC upon activation. In addition, supernatant from DC (SNDC) potently and selectively attracted naive and memory B cells but not germinal center (GC) B cells or other lymphocytes (CD4(+), CD8(+) T cells or NK cells). Production of this activity was restricted to DC and was not increased following DC activation by LPS or CD40 ligand. Surprisingly, the B-cell chemotactic response to SNDC was insensitive to pertussis toxin treatment. In addition, the chemotactic factor(s) appeared resistant to protease digestion and highly sensitive to heat. This suggested that the DC chemotactic factor(s) is different from classical chemoattractants and does not involve G(alpha(i)) proteins on the responding B lymphocytes. It is interesting that SNDC was able to synergize with several chemokines to induce massive migration of B lymphocytes. These observations show that DC spontaneously produce factors that, alone or in cooperation with chemokines, specifically regulate B-cell migration, suggesting a key role of DC in the recruitment or localization of B lymphocytes within secondary lymphoid organs.
authors.affiliation: Inserm U404, Lyon Cedex, France.
authors.forename: B
authors.initials: B
authors.lastname: Dubois
authors.forename: C
authors.initials: C
authors.lastname: Massacrier
authors.forename: C
authors.initials: C
authors.lastname: Caux
mesh_terms: D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D002460:Cell Line; D002478:Cells, Cultured; D018925:Chemokines / Q000494:pharmacology; D002634:Chemotaxis, Leukocyte; D017077:Culture Media, Conditioned / Q000494:pharmacology; D003713:Dendritic Cells / Q000276:immunology*; D004357:Drug Synergism; D018858:Germinal Center / Q000276:immunology; D006801:Humans; D007156:Immunologic Memory; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology; D037342:Pertussis Toxin; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
chemical_list: D018925:Chemokines; D017077:Culture Media, Conditioned; D010566:Virulence Factors, Bordetella; D037342:Pertussis Toxin