title: Chlamydia pneumoniae-infected monocytes exhibit increased adherence to human aortic endothelial cells.
abstract: Interactions between monocytes and endothelial cells play an important role in the pathogenesis of atherosclerosis, and monocyte adhesion to arterial endothelium is one of the earliest events in atherogenesis. Work presented in this study examined human monocyte adherence to primary human aortic endothelial cells following monocyte infection with Chlamydia pneumoniae, an intracellular pathogen associated with atherosclerosis by a variety of sero-epidemiological, pathological and functional studies. Infected monocytes exhibited enhanced adhesion to aortic endothelial cells in a time- and dose-dependent manner. Pre-treatment of C. pneumoniae with heat did not effect the organism's capacity to enhance monocyte adhesion, suggesting that heat-stable chlamydial antigens such as chlamydial lipopolysaccharide (cLPS) mediated monocyte adherence. Indeed, treatment of monocytes with cLPS was sufficient to increase monocyte adherence to endothelial cells, and increased adherence of infected or cLPS-treated monocytes could be inhibited by the LPS antagonist lipid X. Moreover, C. pneumoniae-induced adherence could be inhibited by incubating monocytes with a mAb specific to the human beta 2-integrin chain, suggesting that enhanced adherence resulted from increased expression of these adhesion molecules. These data show that C. pneumoniae can enhance the capacity of monocytes to adhere to primary human aortic endothelial cells. The enhanced adherence exhibited by infected monocytes may increase monocyte residence time in vascular sites with reduced wall shear stress and promote entry of infected cells into lesion-prone locations.
authors.affiliation: Department of Medical Microbiology and Immunology, University of Wisconsin Medical School, 436 SMI, 1300 University Avenue, Madison, WI 53706, USA. gibyrne@facstaff.wisc.edu
authors.forename: M V
authors.initials: MV
authors.lastname: Kalayoglu
authors.forename: B N
authors.initials: BN
authors.lastname: Perkins
authors.forename: G I
authors.initials: GI
authors.lastname: Byrne
mesh_terms: D000911:Antibodies, Monoclonal / Q000627:therapeutic use; D001161:Arteriosclerosis / Q000209:etiology; D018821:CD18 Antigens / Q000502:physiology; D002448:Cell Adhesion; D016993:Chlamydophila pneumoniae / Q000472:pathogenicity*; D004730:Endothelium, Vascular / Q000166:cytology*; D006801:Humans; D008070:Lipopolysaccharides / Q000633:toxicity; D009000:Monocytes / Q000502:physiology*
chemical_list: D000911:Antibodies, Monoclonal; D018821:CD18 Antigens; D008070:Lipopolysaccharides