The 5S rDNA of mussels Mytilus galloprovincialis and M. edulis: sequence variation and chromosomal location.
The 5S ribosomal DNA of the mussels Mytilus galloprovincialis and M. edulis was amplified by PCR using contiguous primers. The most general 5S rDNA amplification pattern consisted of several products in both mussels. Two main PCR products of about 250 bp and 760 bp were cloned and sequenced, revealing two classes of 5S rDNA units. These were characterized as containing an identical coding region of 119 bp but with highly divergent spacers. Clones of each unit type exhibited minimal differences except those of the large unit of M. edulis. The sequences analysed of the two mussels possess the same coding region and only six fixed base changes on the spacers. FISH, carried out with specific probes, consistently showed hybridization signals on the largest metacentric pair (two differentiated sites) and with variable frequency on two other metacentric pairs (one site on each pair). Differences in the 5S rDNA distribution between both mussels were not found. In M. edulis, chromosomes carrying 18S-28S rDNA were also identified by FISH. These correspond to two submetacentric-subtelocentric pairs, as was previously reported in M. galloprovincialis, demonstrating that the two rDNA multigene families are located on different chromosome pairs in these mussels.
Insua
A
A
Departamento de Biología Celular y Molecular, Universidade da Coruña, Spain.
Freire
R
R
Ríos
J
J
Méndez
J
J
D000818:Animals; D001483:Base Sequence; D049872:Bivalvia / Q000235:genetics*; D002874:Chromosome Mapping; D017931:DNA Primers / Q000737:chemistry; D004275:DNA, Ribosomal / Q000235:genetics*; D014644:Genetic Variation; D017404:In Situ Hybridization, Fluorescence; D008677:Metaphase / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D012339:RNA, Ribosomal, 28S / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
D017931:DNA Primers; D004275:DNA, Ribosomal; D012337:RNA, Ribosomal, 18S; D012339:RNA, Ribosomal, 28S