Improved precision of quantitating calcium in biological electron probe analysis.
We describe the use of direct multiple least-squares fitting of (unfiltered) biological X-ray spectra. Test of this method on computer-generated spectra and experimental specimens shows that, for the average biological specimen, direct fitting improves the precision of Ca measurements by an approximate factor of 1.6 for a given electron dose. The influence of non-statistical, instrumental sources of measurement errors is considered, and we show a simple method for reducing the effect of these sources of error in the directly fitted spectra.
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Department of Molecular Physiology and Biological Physics, University of Virginia, P. O. Box 800736, Charlottesville, VA 22908-0736, USA. rh4c@virginia.edu
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Somlyo
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Shao
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D000818:Animals; D002118:Calcium / Q000032:analysis*; D004577:Electron Probe Microanalysis / Q000379:methods*; D006801:Humans; D013048:Specimen Handling
D002118:Calcium