The differential inhibitory effects of genistein on the growth of cervical cancer cells in vitro.
The biological effect of genistein on cervical cancer was studied on two cervical cancer cell lines with different cellular characteristics. Here we report that genistein exhibits inhibitory effects on the growth of HeLa and ME-180 cells. The IC50 was 35 microM and 60 microM for HeLa and ME-180 cells, respectively. ME-180 cells showed obvious G2/M arrest with genistein treatment while most of the HeLa cells were accumulated in S phase. The underlying molecular mechanism was further elucidated by apoptosis analysis and expression levels of cell cycle regulatory proteins. Treatment of the cell lines with genistein also resulted in suppression of invasion through a surrogate membrane in a dose-dependent manner, particularly the HeLa cells. While the underlying mechanism needs to be further studied, the higher suppressive effect on invasion of HeLa cells, an adenocarcinoma cell line, are noteworthy. This in vitro observation may have clinical implication to improve the treatment of cervical adenocarcinoma.
Wang
S Y
SY
Department of Medical Research, Mackay Memorial Hospital, Taipei, Taiwan. eugene@ms2.mmh.org.tw
Yang
K W
KW
Hsu
Y T
YT
Chang
C L
CL
Yang
Y C
YC
D000230:Adenocarcinoma / Q000188:drug therapy; D015153:Blotting, Western; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy; D002453:Cell Cycle / Q000187:drug effects*; D005260:Female; D019833:Genistein / Q000494:pharmacology* / Q000627:therapeutic use; D006131:Growth Inhibitors / Q000494:pharmacology* / Q000627:therapeutic use; D006367:HeLa Cells / Q000187:drug effects; D006801:Humans; D020128:Inhibitory Concentration 50; D014407:Tumor Cells, Cultured / Q000187:drug effects; D002583:Uterine Cervical Neoplasms / Q000188:drug therapy*
D006131:Growth Inhibitors; D019833:Genistein