Production of pyridoxal phosphate by a mutant strain of Schizosaccharomyces pombe.
Conditions for extracellular production of vitamin B6 compounds (B6), especially pyridoxal 5'-phosphate (PLP) by Schizosaccharomyces pombe leul strain were examined. The productivity was dependent on concentration of L-leucine in the culture medium: 30 mg/l gave the highest concentrations of total B6 and PLP. The viable cells harvested at different growth phases showed different productivity: middle and late exponential phase cells showed the highest productivity of total B6 and PLP, respectively. D-Glucose (1%, w/v) among other sugars gave the best productivity. Supplementation of air and ammonium sulfate significantly increased extracellular production of PLP. Superoxide anion producers, menadione and plumbagin, and H202 increased the productivity of PLP. Cycloheximide inhibited the increase of PLP by the oxidative stress and, in contrast, increased pyridoxine.
Chumnantana
R
R
Department of Bioresources Science, Faculty of Agriculture, Kochi University, Nankoku, Japan.
Hirose
K
K
Baba
H
H
Yagi
T
T
D000388:Air; D000645:Ammonium Sulfate / Q000494:pharmacology; D002241:Carbohydrates / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media; D003513:Cycloheximide / Q000494:pharmacology; D005110:Extracellular Space / Q000378:metabolism; D007930:Leucine / Q000494:pharmacology; D009154:Mutation / Q000235:genetics; D009584:Nitrogen / Q000494:pharmacology; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000502:physiology; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D011732:Pyridoxal Phosphate / Q000096:biosynthesis*; D012568:Schizosaccharomyces / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D024483:Vitamin K 3 / Q000494:pharmacology
D002241:Carbohydrates; D003470:Culture Media; D011500:Protein Synthesis Inhibitors; D011732:Pyridoxal Phosphate; D024483:Vitamin K 3; D003513:Cycloheximide; D007930:Leucine; D009584:Nitrogen; D000645:Ammonium Sulfate