Separation of basic drug enantiomers by capillary electrophoresis using ovoglycoprotein as a chiral selector: comparison of chiral resolution ability of ovoglycoprotein and completely deglycosylated ovoglycoprotein.
Separations of basic drug enantiomers by capillary electrophoresis (CE) using ovoglycoprotein (OGCHI) as a chiral selector are described. The effects of running buffer pH and 2-propanol content on the migration times and resolution of basic drug enantiomers were examined using a linear polyacrylamide-coated capillary. High resolution of basic drug enantiomers was attained using a mixture of 50 mM sodium phosphate buffer (pH 4.5-6.0) and 2-propanol (5-30%) including 50 microM OGCHI. It was found that ionic and hydrophobic interactions could work for the recognition of basic drug enantiomers. Further, we compared the chiral resolution ability of OGCHI with that of completely deglycosylated OGCHI (cd-OGCHI) using them as chiral selectors in CE. OGCHI showed higher resolution for basic drug enantiomers tested than cd-OGCHI. The results suggest that the chiral recognition site(s) for OGCHI exists on the protein domain of OGCHI.
Matsunaga
H
H
Faculty of Pharmaceutical Sciences, Mukogawa Women's University, Nishinomiya, Hyogo, Japan.
Haginaka
J
J
D002021:Buffers; D002744:Chlorpheniramine / Q000302:isolation & purification; D019075:Electrophoresis, Capillary / Q000379:methods*; D006020:Glycopeptides / Q000737:chemistry*; D006031:Glycosylation; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D010869:Pindolol / Q000302:isolation & purification; D013237:Stereoisomerism; D014049:Tolperisone / Q000302:isolation & purification; D014299:Trimipramine / Q000737:chemistry
D002021:Buffers; D006020:Glycopeptides; D007202:Indicators and Reagents; C013176:ovoglycopeptide; D002744:Chlorpheniramine; D014299:Trimipramine; D010869:Pindolol; D014049:Tolperisone