Effects of low iron conditions on the repair of DNA lesions induced by Cumene hydroperoxide in Escherichia coli cells.
In the present study, we evaluated the sensitivity of different Escherichia coli strains to Cumene hydroperoxide (CHP) treatment under distinct conditions of Fe2+ availability. Our results showed that the pretreatment with an iron chelator (dipyridyl) protects all the tested strains against CHP toxic effects, but it was not sufficient to abolish the CHP induced mutagenesis. On the other hand, simultaneous pretreatment with both dipyridyl and neocuproine (copper chelator) leads to a complete protection against CHP mutagenic effects. Our data suggest the participation of copper ion in the CHP mutagenesis induced in E. coli.
Asad
L M
LM
Departamento de Biofísica e Biometria, Instituto de Biologia Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro, RJ, Brazil.
Medeiros
D C
DC
Felzenszwalb
I
I
Leitão
A C
AC
Asad
N R
NR
D001555:Benzene Derivatives / Q000494:pharmacology*; D004249:DNA Damage; D004260:DNA Repair / Q000187:drug effects*; D004269:DNA, Bacterial / Q000187:drug effects*; D007501:Iron / Q000494:pharmacology*; D009153:Mutagens / Q000494:pharmacology*
D001555:Benzene Derivatives; D004269:DNA, Bacterial; D009153:Mutagens; D007501:Iron; C007164:cumene hydroperoxide