On-line capillary electrophoresis-electrospray mass spectrometry for the stereoselective analysis of drugs and metabolites.
The on-line combination of partial-filling capillary electrophoresis and electrospray ionization mass spectrometry was demonstrated for the enantioseparation of pharmaceutical drugs and metabolites, namely amphetamines, methadone, venlafaxine and selected tropane alkaloids. The partial-filling technique proved to be a suitable and efficient approach to avoid mass spectrometry (MS) source contamination, as well as signal suppression due to nonvolatile additives. To achieve chiral separation, various chiral selectors were applied, including neutral and particularly negatively charged cyclodextrins. Because of the countercurrent contribution, charged cyclodextrins were found more suitable for the on-line MS detection of separated enantiomers. Hyphenation of capillary electrophoresis (CE) with mass spectrometry was found appropriate for the stereoselective analysis of methadone in real serum samples. Moreover, the use of MS in the selected ion monitoring mode resulted in a very high selectivity, as well as improved sensitivity compared to UV detection. Finally, with atropine as a model compound, the quantitative performances of the method were evaluated and showed high sensitivity, as well as good repeatability in terms of migration time and peak area ratio.
Cherkaoui
S
S
Laboratory of Pharmaceutical Analytical Chemistry, University of Geneva, Switzerland.
Rudaz
S
S
Varesio
E
E
Veuthey
J L
JL
D000662:Amphetamines / Q000302:isolation & purification; D003505:Cyclodextrins / Q000737:chemistry; D003511:Cyclohexanols / Q000302:isolation & purification; D019075:Electrophoresis, Capillary / Q000379:methods*; D007202:Indicators and Reagents; D008691:Methadone / Q000302:isolation & purification; D004364:Pharmaceutical Preparations / Q000032:analysis / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D014326:Tropanes / Q000302:isolation & purification; D000069470:Venlafaxine Hydrochloride
D000662:Amphetamines; D003505:Cyclodextrins; D003511:Cyclohexanols; D007202:Indicators and Reagents; D004364:Pharmaceutical Preparations; D014326:Tropanes; D000069470:Venlafaxine Hydrochloride; D008691:Methadone