[Determination of resibufogenin and cinobufagin in venenum Bufonis by HPLC].
OBJECTIVE
To establish a HPLC method to determine the contents of active constituents in Venenum Bufonis.


METHODS
In determining the contents of resibufogenin and cinobufagin in the traditional Chinese medicine Venenum Bufonis, the mobile phase was acetonitrile-0.5% KH2PO4 solution(50:50)(pH adjusted with value phosphoric acid to 3.25 +/- 0.02).


RESULTS
The constituents thus determined have good linearity and separation. The average recovery of resibufogenin was 100.35%, RSD 1.86%; the average recovery of cinobufagin was 100.38%, RSD 2.09%.


CONCLUSIONS
The method was convenient, rapid, accurate and practicable.
Wang
J
J
Beijing Municipal Institute for Drug Control, Beijing 100035.
Jin
W
W
Jin
H
H
Zhou
F
F
D000664:Amphibian Venoms / Q000737:chemistry*; D000818:Animals; D002018:Bufanolides / Q000032:analysis*; D002023:Bufo bufo; D002025:Bufonidae; D002851:Chromatography, High Pressure Liquid; D008419:Materia Medica / Q000737:chemistry
D000664:Amphibian Venoms; D002018:Bufanolides; D008419:Materia Medica; C005734:bufogenin; C002471:cinobufagin