title: [Mutations of mitochondrial 12S rRNA gene in type 2 diabetes].
abstract: OBJECTIVE
  To investigate mutations at points 1310, 1438 and 1442 of mitochondrial 12S rRNA gene in Chinese type 2 diabetes and screen for new mutations associated with type 2 diabetes in this area.
  
  
  METHODS
  The mitochondrial DNAs (mtDNAs) of 86 patients with type 2 diabetes and those of 70 normal controls were analyzed using PCR-SSCP and PCR product direct sequencing technique.
  
  
  RESULTS
  One patient was found with the mitochondrial DNA G --> A mutation at point 1438, and one with the G two head right arrow A mutation at point 1442, whereas none of the normal controls was found to have mutation at these two points. No C --> T mutation at nucleotide pair 1310 of the mitochondrial 12S rRNA gene was identified.
  
  
  CONCLUSIONS
  The G --> A mutation at points 1438 and 1442 is likely to be associated with the development of type 2 diabetes; the occurrence of the C -->T mutation at point 1310 is probably rare in the blood cells, and the development of type 2 diabetes may be related to heterogeneity in mitochondrial genetic changes.
authors.affiliation: Department of Endocrinology, Affiliated Railway Hospital, Tongji University, Shanghai, 200072 P.R. China. yzy90@yahoo.com.cn
authors.forename: Y
authors.initials: Y
authors.lastname: Yu
authors.forename: J
authors.initials: J
authors.lastname: Shi
authors.forename: S
authors.initials: S
authors.lastname: Shang
authors.forename: X
authors.initials: X
authors.lastname: Teng
authors.forename: W
authors.initials: W
authors.lastname: Li
authors.forename: Z
authors.initials: Z
authors.lastname: Wu
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D004272:DNA, Mitochondrial / Q000235:genetics*; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation; D012335:RNA, Ribosomal / Q000235:genetics*
chemical_list: D004272:DNA, Mitochondrial; D012335:RNA, Ribosomal; C075526:RNA, ribosomal, 12S