Impaired delay but normal trace eyeblink conditioning in PLCbeta4 mutant mice.
To elucidate the functional role of phospholipase Cbeta4 (PLCbeta4), which is highly expressed in the Purkinje cells of the rostral cerebellum, cerebellar long-term depression (LTD) and delay and trace eyeblink conditioning were investigated in PLCbeta4-deficient mice. Rostral cerebellar LTD and delay eyeblink conditioning were severely impaired, whereas trace eyeblink conditioning was not. These results indicate that PLCbeta4 is essential for LTD in the rostral cerebellum and delay conditioning, but not trace conditioning. Rostral cerebellar LTD may be required as a neural substrate for delay conditioning, but is not required for trace conditioning.
Kishimoto
Y
Y
Laboratory of Neurobiophysics, School of Pharmaceutical Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan.
Hirono
M
M
Sugiyama
T
T
Kawahara
S
S
Nakao
K
K
Kishio
M
M
Katsuki
M
M
Yoshioka
T
T
Kirino
Y
Y
D000344:Afferent Pathways / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D003215:Conditioning, Eyelid / Q000502:physiology*; D004576:Electromyography; D019706:Excitatory Postsynaptic Potentials / Q000235:genetics; D005838:Genotype; D007527:Isoenzymes / Q000172:deficiency* / Q000235:genetics; D017774:Long-Term Potentiation / Q000502:physiology*; D051379:Mice; D018345:Mice, Knockout; D009119:Muscle Contraction / Q000235:genetics; D009433:Neural Inhibition / Q000502:physiology*; D009924:Organ Culture Techniques; D018408:Patch-Clamp Techniques; D054799:Phospholipase C beta; D011689:Purkinje Cells / Q000166:cytology / Q000378:metabolism*; D011930:Reaction Time / Q000502:physiology*; D013569:Synapses / Q000235:genetics; D009435:Synaptic Transmission / Q000235:genetics; D010738:Type C Phospholipases / Q000172:deficiency* / Q000235:genetics
D007527:Isoenzymes; D010738:Type C Phospholipases; D054799:Phospholipase C beta