Upregulation of matrix metalloproteinase-1 production by prostaglandin F2alpha in human gingival fibroblasts.
In the present study, we investigated the effect of prostglandin F2alpha (PGF2alpha) and combination of PGF2alpha and interleukin(IL)-1beta on matrix metalloproteinase (MMP)-1 production in human gingival fibroblasts (HGF). PGF2alpha enhanced MMP-1 production in a dose-dependent manner in HGF. Combination of PGF2alpha and IL-1beta induced a synergistic increase of MMP-1 production in HGF. Furthermore, fluprostenol, a specific FP receptor agonist, increased MMP-1 production and induced a synergistic enhancement of IL-1beta-induced MMP-1 production in HGF, similar to PGF2alpha. FP receptor mRNA expression was detected in HGF, by reverse transcription-polymerase chain reaction. Northern blot analysis revealed that PGF2alpha enhanced MMP-1 mRNA expression in HGF and that PGF2alpha increased MMP-1 mRNA levels induced by IL-1beta. In conclusion, we suggest that PGF2alpha increases MMP-1 production in HGF and synergistically enhances MMP-1 production in IL-1beta-stimulated HGF. PGF2alpha may be involved in degradation of connective tissue in periodontal lesions.
Noguchi
K
K
Department of Hard Tissue Engineering, Graduate School, Tokyo Medical and Dental University, Japan. kazuyuki-noguchi.peri@dent.tmd.ac.jp
Tominaga
Y
Y
Matsushita
K
K
Izumi
Y
Y
Endo
H
H
Kondo
H
H
Ishikawa
I
I
D015152:Blotting, Northern; D002478:Cells, Cultured; D015237:Dinoprost / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations; D004790:Enzyme Induction; D005347:Fibroblasts / Q000187:drug effects / Q000201:enzymology; D005881:Gingiva / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology; D020781:Matrix Metalloproteinase 1 / Q000096:biosynthesis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019714:Tissue Inhibitor of Metalloproteinases / Q000378:metabolism; D015854:Up-Regulation
D004338:Drug Combinations; D007375:Interleukin-1; D019714:Tissue Inhibitor of Metalloproteinases; D015237:Dinoprost; D020781:Matrix Metalloproteinase 1