title: Establishment and characterization of a cell line, MT-213-VGH, isolated from a mixed müllerian tumor of the uterus.
abstract: OBJECTIVE
  To establish a cell line from a woman with malignant mixed müllerian tumor of the uterus and to examine the biologic properties of this cell line (MT-213-VGH).
  
  
  METHODS
  Cells were cloned by the limiting dilution method. Histologic staining of mixed müllerian (mesodermal) tumor (MMMT) cells was performed with May-Grünwald-Giemsa and hematoxylin and eosin stain. After more than 20 passages, cells were used to estimate the population-doubling time and colony-forming efficiency of MMMT cells. The cell line exhibited considerable variation in the degree of sensitivity to diverse chemotherapy drugs in vitro.
  
  
  RESULTS
  MMMT cells containing antigens for vimentin and myoglobin were detected, but those for CA-125, carcinoembryonic antigen, cytoskeleton, desmin, epithelial membrane antigen and fibronectin were not found. In addition, MT-213-VGH cells contained a mucinous substance; its chromosome model number is 45. This cell line showed differential sensitivities to chemotherapeutic agents, such as bleomycin, cisplatin, 5-fluorouracil and vinblastine.
  
  
  CONCLUSIONS
  The establishment and availability of the number cell line MT-213-VGH for a malignant mixed müllerian tumor of the uterus should assist in research on new methods of managing this type of gynecologic cancer.
authors.affiliation: Department of Obstetrics and Gynecology, Veterans General Hospital-Taipei and National Yang-Ming University School of Medicine, Taiwan.
authors.forename: K C
authors.initials: KC
authors.lastname: Chao
authors.forename: P H
authors.initials: PH
authors.lastname: Wang
authors.forename: C C
authors.initials: CC
authors.lastname: Chang
authors.forename: C R
authors.initials: CR
authors.lastname: Lai
authors.forename: H T
authors.initials: HT
authors.lastname: Ng
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology; D018929:Cell Culture Techniques / Q000379:methods; D005260:Female; D006801:Humans; D008875:Middle Aged; D018200:Mixed Tumor, Mullerian / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D013194:Staining and Labeling / Q000379:methods; D014407:Tumor Cells, Cultured; D014594:Uterine Neoplasms / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology
chemical_list: D000970:Antineoplastic Agents