Porins from Salmonella typhimurium accelerate human blood coagulation in vitro by selective stimulation of thrombin activity: implications in septic shock DIC pathogenesis.
The effect of porins, major hydrophobic outer membrane proteins purified from Salmonella typhimurium, on human blood coagulation was investigated. It was found that micromolar concentrations of porins accelerated markedly human blood coagulation in vitro. Using appropriate experiments, data were obtained showing that the main target of the porin-induced procoagulant effect was thrombin. A possible binding of porins with thrombin has been suggested to be the basis of this effect. The implications of this finding in the pathogenesis of the disseminated intravascular coagulation syndrome (DIC) occurring during the Gram-negative septic shock is discussed.
Di Micco
B
B
Dipartimento di Biochimica e Biofisica Francesco Cedrangolo and Centro di Ricerca Interdipartimentale di Scienze Computazionali e Biotecnologiche, Seconda Università degli Studi di Napoli, Napoli, Italy.
Metafora
S
S
Colonna
G
G
Cartenì
M
M
Ragone
R
R
Macalello
M A
MA
Di Micco
P
P
Baroni
A
A
Catalanotti
P
P
Tufano
M A
MA
D000990:Antithrombin III / Q000378:metabolism; D000991:Antithrombins / Q000378:metabolism; D001777:Blood Coagulation; D004211:Disseminated Intravascular Coagulation / Q000209:etiology / Q000503:physiopathology*; D006801:Humans; D010314:Partial Thromboplastin Time; D010447:Peptide Hydrolases / Q000378:metabolism; D018272:Porins / Q000378:metabolism* / Q000494:pharmacology / Q000502:physiology; D011517:Prothrombin Time; D012486:Salmonella typhimurium / Q000378:metabolism*; D012772:Shock, Septic / Q000378:metabolism / Q000503:physiopathology; D013577:Syndrome; D013917:Thrombin / Q000378:metabolism*; D014914:Whole Blood Coagulation Time
D000991:Antithrombins; D018272:Porins; C046193:antithrombin III-protease complex; D000990:Antithrombin III; D010447:Peptide Hydrolases; D013917:Thrombin