Structures of unliganded and ATP-bound states of the Escherichia coli chaperonin GroEL by cryoelectron microscopy.
We have developed an angular refinement procedure incorporating correction for the microscope contrast transfer function, to determine cryoelectron microscopy (cryo-EM) structures of the Escherichia coli chaperonin GroEL in its apo and ATP-bound forms. This image reconstruction procedure is verified to 13-A resolution by comparison of the cryo-EM structure of unliganded GroEL with the crystal structure. Binding, encapsulation, and release of nonnative proteins by GroEL and its cochaperone GroES are controlled by the binding and hydrolysis of ATP. Seven ATP molecules bind cooperatively to one heptameric ring of GroEL. This binding causes long-range conformational changes that determine the orientations of remote substrate-binding sites, and it also determines the conformation of subunits in the opposite ring of GroEL, in a negatively cooperative mechanism. The conformation of GroEL-ATP was determined at approximately 15-A resolution. In one ring of GroEL-ATP, the apical (substrate-binding) domains are extremely disordered, consistent with the high mobility needed for them to achieve the 60 degrees elevation and 90 degrees twist of the GroES-bound state. Unexpectedly, ATP binding also increases the separation between the two rings, although the interring contacts are present in the density map.
Roseman
A M
AM
Department of Crystallography, Birkbeck College London, Malet Street, London, WC1E 7HX, United Kingdom.
Ranson
N A
NA
Gowen
B
B
Fuller
S D
SD
Saibil
H R
HR
D000255:Adenosine Triphosphate / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D001665:Binding Sites / Q000187:drug effects; D018834:Chaperonin 60 / Q000737:chemistry* / Q000378:metabolism; D020285:Cryoelectron Microscopy / Q000379:methods*; D003460:Crystallization; D029968:Escherichia coli Proteins / Q000737:chemistry; D021621:Imaging, Three-Dimensional; D011487:Protein Conformation / Q000187:drug effects
D018834:Chaperonin 60; D029968:Escherichia coli Proteins; D000255:Adenosine Triphosphate