title: Detection of Fcgamma binding protein antigen in human sera and its relation with autoimmune diseases.
abstract: Intestinal goblet cells of patients with ulcerative colitis and Crohn's disease highly express a binding protein of the Fc portion of IgG (FcgammaBP), which is entirely different from the Fcgamma receptors I,II, and III on neutrophils and macrophages. In this study, we proved the qualitative existence of FcgammaBP antigen in the sera of patients with rheumatoid arthritis and systemic lupus erythematosus, and, further, established a highly sensitive and quantitatively reproducible assay for FcgammaBP antigen in order to prevent the cross-reactivity of FcgammaBP with von Willebrand factor which has about 30% homology. This assay revealed a higher level of FcgammaBP antigen in the blood stream of patients with autoimmune diseases, especially progressive systemic sclerosis. This would suggest that abnormal production of autoantibodies reflects increased generation of FcgammaBP in goblet cells and its secretion into the circulation by an unknown mechanism.
authors.affiliation: Tokyo Institute for Immunopharmacology, Chugai Pharmaceutical Co. Ltd., 171, Tokyo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Kobayashi
authors.forename: M
authors.initials: M
authors.lastname: Yagasaki
authors.forename: N
authors.initials: N
authors.lastname: Harada
authors.forename: K
authors.initials: K
authors.lastname: Chichibu
authors.forename: T
authors.initials: T
authors.lastname: Hibi
authors.forename: T
authors.initials: T
authors.lastname: Yoshida
authors.forename: W R
authors.initials: WR
authors.lastname: Brown
authors.forename: M
authors.initials: M
authors.lastname: Morikawa
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001172:Arthritis, Rheumatoid / Q000097:blood / Q000276:immunology; D001324:Autoantigens / Q000097:blood / Q000276:immunology*; D001327:Autoimmune Diseases / Q000097:blood / Q000276:immunology*; D016466:CHO Cells; D002352:Carrier Proteins / Q000097:blood / Q000276:immunology*; D015815:Cell Adhesion Molecules; D006224:Cricetinae; D003429:Cross Reactions / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D000882:Haplorhini; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D008180:Lupus Erythematosus, Systemic / Q000097:blood / Q000276:immunology; D008565:Membrane Proteins; D011233:Precipitin Tests; D011817:Rabbits; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014841:von Willebrand Factor / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D001324:Autoantigens; D002352:Carrier Proteins; D015815:Cell Adhesion Molecules; C106607:FCGBP protein, human; D007106:Immune Sera; D008565:Membrane Proteins; D014841:von Willebrand Factor