title: Correlation of gas chromatographic properties of chlorobenzenes and polychlorinated biphenyls with the occurrence of reductive dechlorination by untamed microorganisms.
abstract: To understand the dechlorination ability of chlorobenzenes (CBs) and polychlorinated biphenyls (PCBs) by untamed microorganisms under anaerobic condition and to correlate gas chromatographic properties with the occurrence of reductive dechlorination, introduction of CBs and PCBs in the culture medium inoculated with microorganisms from sludge and sediment, respectively, were performed. Three kinds of culture media preparing from sludge, river water and a synthetic medium were used in the experiments. HCB was degraded to 1,3,5-trichlorobenzene (1,3,5-TCB) and 1,3-dichlorobenzene (1,3-DCB) in both sludge medium and synthetic medium with inoculated microorganisms. Three PCB congeners including 2,3,4-, 3,4,5- and 2,3,4,5-CBp (chlorinated biphenyl) were not found to be dechlorinated in the river water medium with inoculation culture but to be dechlorinated in the synthetic medium. MNDO methodology was used to compute theoretical dechlorination reaction heats and GC-ECD techniques were used to estimate chromatographic data of CB and PCB congeners. Both CB and PCB congeners showed that dechlorination by untamed microorganisms under anaerobic mixed cultures were more likely to occur when larger amounts of energy were released and greater deltaln RRT value between the parent congener and the daughter product was observed. Deltaln RRT provided a more precise information on the singularity of PCBs ortho-dechlorination in an aspect of thermodynamic favorable rule.
authors.affiliation: Department of Agricultural Chemistry, National Taiwan University, Taipei, ROC.
authors.forename: I M
authors.initials: IM
authors.lastname: Chen
authors.forename: F C
authors.initials: FC
authors.lastname: Chang
authors.forename: Y S
authors.initials: YS
authors.lastname: Wang
mesh_terms: D001421:Bacteria, Anaerobic; D002722:Chlorobenzenes / Q000032:analysis / Q000378:metabolism*; D002849:Chromatography, Gas; D004785:Environmental Pollutants / Q000032:analysis / Q000378:metabolism*; D019015:Geologic Sediments / Q000382:microbiology; D007700:Kinetics; D011078:Polychlorinated Biphenyls / Q000032:analysis / Q000378:metabolism*; D012722:Sewage / Q000382:microbiology; D013696:Temperature; D018508:Water Purification
chemical_list: D002722:Chlorobenzenes; D004785:Environmental Pollutants; D012722:Sewage; D011078:Polychlorinated Biphenyls