Expression of cyclin kinase inhibitor p27(Kip1) in skin tumours of dogs.
Skin tumours (n=148) of epidermal or hair follicle origin were examined immunohistochemically to determine the expression of p27(Kip1)(p27), a cyclin-dependent kinase inhibitor (CDKI), and of Ki-67. In normal skin, a large number of basal cells of the epidermis and hair follicles were positive for Ki-67 and many suprabasal epithelial cells were positive for p27. Most of the hair matrix cells were positive for Ki-67 but negative for p27. Hair papillae were strongly positive for p27. Squamous cell carcinomas had a p27 positive index (PI) significantly lower than that of trichoepitheliomas (P<0.005), basal cell tumours (P<0.05) and intracutaneous cornifying epitheliomas (P<0.001). In contrast, Ki-67 PIs of squamous cell carcinomas and pilomatrixomas were significantly higher than those of trichoepitheliomas, basal cell tumours and intracutaneous cornifying epitheliomas (P<0.01 to P<0.001). No significant difference was observed between the Ki-67 PI values of squamous cell carcinomas and pilomatrixomas. The results suggested that p27 is capable of suppressing cell proliferation in the differentiation of normal canine skin. In spite of being a benign neoplasm, pilomatrixomas had a low p27 expression; this may be a reflection of the proliferative potential of the hair matrix. The expression of p27 may be a useful marker for the analysis of cell kinetics.
Sakai
H
H
Department of Veterinary Pathology, Faculty of Agriculture, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
Yamane
T
T
Yanai
T
T
Shirai
N
N
Masegi
T
T
D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000473:pathology / Q000662:veterinary*; D002452:Cell Count; D018797:Cell Cycle Proteins / Q000032:analysis / Q000096:biosynthesis*; D050760:Cyclin-Dependent Kinase Inhibitor p27; D018844:Cyclin-Dependent Kinases / Q000037:antagonists & inhibitors*; D004283:Dog Diseases / Q000378:metabolism* / Q000473:pathology; D004285:Dogs; D006201:Hair Diseases / Q000378:metabolism / Q000473:pathology / Q000662:veterinary*; D007124:Immunoenzyme Techniques / Q000662:veterinary; D019394:Ki-67 Antigen / Q000096:biosynthesis; D018296:Pilomatrixoma / Q000378:metabolism / Q000473:pathology / Q000662:veterinary*; D012867:Skin / Q000378:metabolism; D012878:Skin Neoplasms / Q000378:metabolism / Q000473:pathology / Q000662:veterinary*; D025521:Tumor Suppressor Proteins / Q000032:analysis / Q000096:biosynthesis*
D018797:Cell Cycle Proteins; D019394:Ki-67 Antigen; D025521:Tumor Suppressor Proteins; D050760:Cyclin-Dependent Kinase Inhibitor p27; D018844:Cyclin-Dependent Kinases