Tetrahydrobiopterin inhibits copper-induced oxidation of low density lipoprotein.
The effect of tetrahydrobiopterin (BPH4) on Cu2+-induced oxidation of low density lipoprotein (LDL) prepared from rabbit plasma was examined. BPH4 (0.5-10 microM) inhibited the formation of thiobarbituric acid-reactive substances (TBARS) in LDL induced by 5 microM Cu2+ in a dose-dependent manner. BPH4 also suppressed the increase in relative electrophoretic mobility (REM) of LDL in the presence of 5 microM Cu2+. The potency of inhibitions of BPH4 on TBARS formation and REM was the same as or stronger than that of ascorbic acid and alpha-tocopherol, which are known as endogenous inhibitors of LDL oxidation. These results suggest that BPH4 could act as an efficacious endogenous inhibitor of atherogenic LDL modification.
Sakuma
S
S
Department of Hygienic Chemistry, Osaka University of Pharmaceutical Sciences Takatsuki, Japan. sakuma@oysun01.oups.ac.jp
Fujimoto
Y
Y
Gohda
Y
Y
Fujita
T
T
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001708:Biopterins / Q000031:analogs & derivatives* / Q000494:pharmacology*; D003300:Copper / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D008077:Lipoproteins, LDL / Q000187:drug effects / Q000378:metabolism*; D010084:Oxidation-Reduction / Q000187:drug effects; D011817:Rabbits
D000975:Antioxidants; D008077:Lipoproteins, LDL; D001708:Biopterins; D003300:Copper; C003402:sapropterin