CXCR4 is the primary receptor for feline immunodeficiency virus in astrocytes.
Feline astrocytes were productively infected with the Crandell feline kidney (CrFK) cell-adapted feline immunodeficiency virus (FIV) Petaluma strain in a primary culture. They expressed mRNA of CXCR4, and the FIV infection was blocked by stromal cell-derived factor 1alpha (SDF-1alpha), SDF-1beta, or the bicyclam AMD3100 in a dose-dependent manner. These observations suggest that, like FIV infection in CrFK cells and lymphocytes, the virus uses CXCR4 as a primary receptor for infecting astrocytes and this can be a possible natural model for AIDS dementia complex.
Nakagaki
K
K
National Institute of Neuroscience, NCNP Kodaira, Tokyo, Japan.
Nakagaki
K
K
Takahashi
K
K
Schols
D
D
De Clercq
E
E
Tabira
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T
D015526:AIDS Dementia Complex; D000818:Animals; D001253:Astrocytes / Q000378:metabolism / Q000821:virology*; D001596:Benzylamines; D002415:Cats; D002478:Cells, Cultured; D054377:Chemokine CXCL12; D019743:Chemokines, CXC / Q000494:pharmacology; D000080027:Cyclams; D016207:Cytokines / Q000494:pharmacology; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D015683:Gene Products, gag / Q000096:biosynthesis / Q000235:genetics; D006571:Heterocyclic Compounds / Q000494:pharmacology; D016089:Immunodeficiency Virus, Feline / Q000502:physiology*; D017628:Microglia / Q000378:metabolism / Q000821:virology; D009419:Nerve Tissue Proteins / Q000502:physiology*; D012333:RNA, Messenger / Q000096:biosynthesis; D019718:Receptors, CXCR4 / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D011991:Receptors, Virus / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D013047:Specific Pathogen-Free Organisms
D001596:Benzylamines; D054377:Chemokine CXCL12; D019743:Chemokines, CXC; D000080027:Cyclams; D016207:Cytokines; D015683:Gene Products, gag; D006571:Heterocyclic Compounds; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D019718:Receptors, CXCR4; D011991:Receptors, Virus; C074662:p24 protein, Feline immunodeficiency virus; C088327:plerixafor