title: Enhanced glial activation and expression of specific CNS inflammation-related molecules in aged versus young rats following cortical stab injury.
abstract: Aging is associated with increased glial responsiveness that may enhance the brain's susceptibility to injury and disease. To determine whether unique age-related molecular responses occur in brain injury, we assessed mRNA levels of representative central nervous system (CNS) inflammation-related molecules in young (3 months) and aged (36 months) Fisher 344/Brown Norwegian F1 hybrid rats following cortical stab. Enhanced glial activation in older animals was accompanied by increased expression of a subset of inflammation-related mRNAs, including IL-1beta, TNFalpha, IL-6, ICAM-1, inducible nitric oxide synthase (iNOS), metalloproteinase-9 (MMP-9), and complement 3alpha-chain 1 (C3alpha1). Recognition of these age-specific differences may guide development of novel treatment regimes for older individuals.
authors.affiliation: Department of Neurobiology and Anatomy, University of Rochester, 601 Elmwood Avenue, Box 603, Rochester, NY 14642, USA.
authors.forename: S
authors.initials: S
authors.lastname: Kyrkanides
authors.forename: M K
authors.initials: MK
authors.lastname: O'Banion
authors.forename: P E
authors.initials: PE
authors.lastname: Whiteley
authors.forename: J C
authors.initials: JC
authors.lastname: Daeschner
authors.forename: J A
authors.initials: JA
authors.lastname: Olschowka
mesh_terms: D000375:Aging / Q000276:immunology*; D000818:Animals; D001253:Astrocytes / Q000737:chemistry / Q000276:immunology*; D001921:Brain / Q000276:immunology; D001930:Brain Injuries / Q000276:immunology*; D015926:Complement C3a / Q000235:genetics; D017931:DNA Primers; D015870:Gene Expression / Q000276:immunology; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics; D007375:Interleukin-1 / Q000235:genetics; D015850:Interleukin-6 / Q000235:genetics; D020780:Matrix Metalloproteinase 9 / Q000235:genetics; D017628:Microglia / Q000737:chemistry / Q000276:immunology*; D019001:Nitric Oxide Synthase / Q000235:genetics; D052247:Nitric Oxide Synthase Type II; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D011914:Rats, Inbred BN; D011916:Rats, Inbred F344; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics; D014951:Wounds, Stab / Q000276:immunology
chemical_list: D017931:DNA Primers; D005904:Glial Fibrillary Acidic Protein; D007375:Interleukin-1; D015850:Interleukin-6; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D018799:Intercellular Adhesion Molecule-1; D015926:Complement C3a; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496318:Nos2 protein, rat; D020780:Matrix Metalloproteinase 9