[Changes in TGF-beta 1 and type I, III procollagen gene expression in keloid and hypertrophic scar].
OBJECTIVE
The expression of mRNA for TGF-beta 1, type I, III procollagen in keloid and hypertrophic scar was investigated in order to elucidate the pathogenesis of and the difference between keloid and hypertrophic scar.


METHODS
Dot-blot hybridization analysis was used to examine the type I, III procollagen and the expression levels of steady-state mRNA. In situ hybridization allowed direct assessment of the distribution of TGF-beta 1 mRNA in the tissue.


RESULTS
1. Our study indicated that the expression level of mRNA for TGF-beta 1 significantly increased in keloid and hypertrophic scar compared with normal scar and normal skin. 2. In keloid tissues, type I precollagen mRNA expression was selectively increased. However, in hypertrophic scar, type I and III collagen mRNAs expressions were simultaneously increased.


CONCLUSIONS
TGF-beta 1 plays an important role in the pathogenesis of keloid and hypertrophic scarring. It implicates that distinct molecular mechanisms are operative in the development of keloid and hypertrophic scarring.
Tang
S
S
Department of Burn and Plastic Surgery, Second Affiliated Hospital of Shantou University Medical College, Shantou 515041.
Pang
S
S
Cao
Y
Y
D017439:Cicatrix, Hypertrophic / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007627:Keloid / Q000235:genetics / Q000378:metabolism*; D011347:Procollagen / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D016212:Transforming Growth Factor beta / Q000096:biosynthesis* / Q000235:genetics
D011347:Procollagen; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta