Na,K-ATPase expression in the mouse cochlea is not dependent on the mineralocorticoid receptor.
This study was performed in order to test the hypothesis that the mineralocorticoid hormone stimulates the expression of Na,K-ATPase in the cochlea of the mouse. Immunohistochemistry was used to investigate the distribution of the mineralocorticoid receptor (MR) in the cochlea of the C57Bl/J6 mouse at different ages between gestational day 19 and postnatal day 30, and the occurrence and distribution of Na,K-ATPase in the inner ear of a mouse with a null mutation of the MR. Adult patterns of staining for MR were found as early as on gestational day 19 in the cochlea, with small changes thereafter. MR was detected in the same structures in the cochlea as Na,K-ATPase in earlier studies, where the amount of Na,K-ATPase increased after postnatal day 4. Thus there is latency between the increase of MR and the increase of Na,K-ATPase. In the cochlea of the MR deficient mouse, antibody labelling of Na,K-ATPase showed no significant difference as compared to the control wild type mouse. The hypothesis that mineralocorticoid hormone alone via MR stimulates the formation of Na,K-ATPase in the inner ear could not be confirmed by this study, and other regulating mechanisms must be considered.
Erichsen
S
S
Department of Otorhinolaryngology, Karolinska Hospital, Stockholm, Sweden.
Berger
S
S
Schmid
W
W
Stierna
P
P
Hultcrantz
M
M
D000818:Animals; D000831:Animals, Newborn; D003051:Cochlea / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D004710:Endolymph / Q000378:metabolism; D007150:Immunohistochemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D018161:Receptors, Mineralocorticoid / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*
D018161:Receptors, Mineralocorticoid; D000254:Sodium-Potassium-Exchanging ATPase