Antibody-dependent enhancement of coxsackievirus B4 infectivity of human peripheral blood mononuclear cells results in increased interferon-alpha synthesis.
IgG devoid of neutralizing activity and isolated from donor plasma by chromatography formed immune complexes with coxsackievirus B4 (CVB4) and significantly increased the infection of peripheral blood mononuclear cells with CVB4. The major host cells for CVB4 infection enhanced with IgG are monocytic CD14+ cells. The roles of CVB and adenovirus receptor and Fcgamma receptor II and III have been shown. Increased viral replication and the release of infectious particles were demonstrated when interferon (IFN)-alpha produced by infected cells was first neutralized by use of antibodies. The CVB4 IgG-induced synthesis of IFN-alpha by monocytes reflected entry and uncoating of CVB4 but not of viral replication and required the presence of CVB4 RNA inside the cells. Thus, CVB4 can infect monocytes by an antibody-dependent mechanism through interactions between the virus, antiviral antibodies, and specific receptors that result in IFN-alpha production.
Hober
D
D
Laboratoire de Virologie, Centre Hospitalier Régional et Universitaire, 59037 Lille Cedex, France. dhober@chru-lille.fr
Chehadeh
W
W
Bouzidi
A
A
Wattré
P
P
D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology*; D019067:Antibody-Dependent Enhancement; D002478:Cells, Cultured; D029822:Enterovirus B, Human / Q000276:immunology* / Q000472:pathogenicity*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D016898:Interferon-alpha / Q000096:biosynthesis*; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000821:virology*; D018950:Lipopolysaccharide Receptors / Q000378:metabolism; D009000:Monocytes / Q000821:virology; D009500:Neutralization Tests; D012367:RNA, Viral / Q000235:genetics / Q000276:immunology; D014162:Transfection; D014771:Virion / Q000502:physiology
D000914:Antibodies, Viral; D007074:Immunoglobulin G; D016898:Interferon-alpha; D018950:Lipopolysaccharide Receptors; D012367:RNA, Viral