Reactor optimization for alpha-1,2 glucooligosaccharide synthesis by immobilized dextransucrase.
The immobilization of dextransucrase in Ca-alginate beads relies on the close association between dextran polymer and dextransucrase. However, high amounts of dextran in the enzyme preparation drastically limit the specific activity of the immobilized enzyme (4 U/mL of alginate beads). Moreover, even in the absence of diffusion limitation at the batch conditions used, the enzyme behavior is modified by entrapment so that the dextran yield increases and the alpha-1,2 glucooligosaccharides (GOS) are produced with a lower yield (46.6% instead of 56.7%) and have a lower mean degree of polymerization than with the free dextransucrase. When the immobilized catalyst is used in a continuous reaction, the reactor flow rate necessary to obtain high conversion of the substrates is very low, leading to external diffusion resistance. As a result, dextran synthesis is even higher than in the batch reaction, and its accumulation within the alginate beads limits the operational stability of the catalyst and decreases glucooligosaccharide yield and productivity. This effect can be limited by using reactor columns with length to diameter ratio > or =20, and by optimizing the substrate concentrations in the feed solution: the best productivity obtained was 3.74 g. U(-1). h(-1), with an alpha-1,2 GOS yield of 36%.
Dols-Lafargue
M
M
Laboratoire de Biochimie et Technologie des Aliments, Avenue des Facultés, 33405, Talence cedex France.
Willemot
R M
RM
Monsan
P F
PF
Remaud-Simeon
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M
D019149:Bioreactors; D002851:Chromatography, High Pressure Liquid; D004800:Enzymes, Immobilized / Q000378:metabolism*; D005964:Glucosyltransferases / Q000378:metabolism*; D007934:Leuconostoc / Q000201:enzymology* / Q000378:metabolism; D009844:Oligosaccharides / Q000096:biosynthesis*
D004800:Enzymes, Immobilized; D009844:Oligosaccharides; D005964:Glucosyltransferases; C020025:dextransucrase