[Genetic analysis of cry1Ab gene of Bt rice].
Improved histochemical staining for GUS activity, PCR and Western blotting were used to analyse the progeny population of Bt rice crossed with conventional rice varieties. A total of 392 plants expressing Bt toxin protein were found in 394 GUS positive plants. The result demonstrated that cry1Ab gene closely inherited and expressed with reporter gene gus. GUS assays indicated that cry1Ab gene of Bt rice inherits as a single dorminant gene. Southern hybridization confirmed cry1Ab gene can inherit stabily in the progenies of Bt rice. Mendelian segregation of reporter gene gus was also observed in F2, BC1 and BC1F2 progenies, which indicated that cry1Ab gene inherits as a single dorminant gene in the progenies of Bt rice crossed with conventional rice varieties.
Wang
Z H
ZH
Institute of Nuclear Agricultural Sciences, Zhejiang University, Hangzhou 310029, China.
Wu
G
G
Cui
H R
HR
Altosaar
I
I
Xia
Y W
YW
Shu
Q Y
QY
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000235:genetics*; D001427:Bacterial Toxins; D003433:Crosses, Genetic; D004731:Endotoxins / Q000235:genetics*; D005799:Genes, Dominant; D006460:Hemolysin Proteins; D012275:Oryza / Q000235:genetics*; D030821:Plants, Genetically Modified
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis