[Histological study and stereologic analysis of membranous versus endochondral onlay bone grafts].
OBJECTIVE
The experimental study was to determine the differences in changes in the onlay bone grafts of different embryonic origin.


METHODS
The study was designed to compare the potential for appositional bone growth in membranous and endochondral onlay grafts in New-Zealand rabbits and a rhesus monkey. Results were assessed with volumetric measurement, histological examinations and stereologic analysis with double-fluorochrome labeling technique.


RESULTS
For the membranous and endochondral bone grafts, the volumetric retention rate was (78.4 +/- 3.5)% and (56.0 +/- 5.1)% respectively. The mineralization apposition rate (MAR) was (3.6 +/- 0.8) microns/d and (0.92 +/- 0.33) micron/d; the osteoid seam width (OSW) was (14.56 +/- 2.69) microns and (7.38 +/- 2.20) microns; the tetracycline uptake rate(TUA) was (91.22 +/- 2.69)% and (51.28 +/- 4.11)%, respectively.


CONCLUSIONS
1. It was found that the membranous bone graft maintained its volume to a significantly greater extent than the endochondral bone graft; 2. Membranous bone grafts exhibited higher survival rate and greater activity than endochondral bone grafts; 3. Different changes were also found in the two recipient sites. There was more powerful bone regenerative reaction in the recipient bed of the membranous bone graft than the endochondral one.
Yang
B
B
Plastic Surgery Hospital of Chinese Academy of Medical Sciences, BaDaChu, Beijing 100041.
Zhao
M
M
Xiong
B
B
D000818:Animals; D002356:Cartilage; D007085:Ilium / Q000473:pathology / Q000637:transplantation*; D008253:Macaca mulatta; D008297:Male; D009055:Mouth / Q000601:surgery; D009666:Nose / Q000601:surgery; D010006:Osteoblasts / Q000473:pathology*; D011817:Rabbits; D012886:Skull / Q000473:pathology / Q000637:transplantation*