Killing effect of interleukin-2 and interferon-alpha activated leukemic bone marrow in remission on K562 leukemic cells.
OBJECTIVE
To evaluate the killing effect of interleukin-2 (IL-2) and interferon-alpha (IFN-alpha) activated bone marrow cells on K562 cells.


METHODS
Semi-solid colony and 3H-TdR incorporating method were used.


RESULTS
Bone marrow from leukemia patients in remission was activated in vitro with IL-2 for 3 days. The activated bone marrow (ABM) displayed killing effects of 0.31-2.30 logs on K562 cells. This killing effect was further increased to 0.30-3.15 logs when IFN-alpha added with IL-2 to the marrow for activation. IL-2 alone or in combination with IFN-alpha showed no inhibition of CFU-GM and K562 cells. Compared with IL-2 or IFN-alpha alone, the combination of the two cytokines could more effectively maintain the killing effect of ABM on leukemic cells.


CONCLUSIONS
IFN-alpha can augment the purging effect of IL-2 ABM and combination of the two cytokines can effectively maintain the cytotoxicity of ABM.
Li
C
C
Institute of Hematology, Blood Diseases Hospital, CAMS and PUMC, Tianjin 300020, China.
Yan
W
W
Hao
Y
Y
Han
M
M
Qiu
L
L
Liu
H
H
Han
J
J
Li
X
X
Feng
S
S
D000208:Acute Disease; D001854:Bone Marrow Cells / Q000276:immunology*; D016465:Bone Marrow Purging; D016026:Bone Marrow Transplantation; D006412:Hematopoietic Stem Cells; D006801:Humans; D016898:Interferon-alpha / Q000494:pharmacology*; D007376:Interleukin-2 / Q000494:pharmacology*; D020014:K562 Cells / Q000473:pathology; D015979:Killer Cells, Lymphokine-Activated / Q000276:immunology*; D007938:Leukemia / Q000473:pathology*; D012074:Remission Induction
D016898:Interferon-alpha; D007376:Interleukin-2