title: Leukemia inhibitory factor induces nociceptin mRNA in cultured rat cortical neurons.
abstract: The effects of leukemia inhibitory factor (LIF) on the expression of neurotransmitter synthetase and neuropeptide mRNAs in cultured rat cortical neurons were examined by reverse transcription-polymerase chain reaction. Nociceptin mRNA expression was increased by treatment with 20 or 80 ng/ml LIF for 24 h, but choline acetyl transferase, glutamic acid decarboxylase, enkephalin, dynorphin, substance P, somatostatin and galanin mRNA expression were not altered by LIF. These observations indicated a specific effect of LIF on nociceptin gene regulation in cultured cortical neurons.
authors.affiliation: Department of Molecular Pharmacology, Faculty of Pharmaceutical Sciences, Kyoto University, 606-8501, Kyoto, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Minami
authors.forename: H
authors.initials: H
authors.lastname: Yamakuni
authors.forename: Y
authors.initials: Y
authors.lastname: Ohtani
authors.forename: M
authors.initials: M
authors.lastname: Okada
authors.forename: J
authors.initials: J
authors.lastname: Nakamura
authors.forename: M
authors.initials: M
authors.lastname: Satoh
mesh_terms: D000375:Aging / Q000235:genetics; D000818:Animals; D015703:Antigens, CD / Q000235:genetics; D002478:Cells, Cultured / Q000187:drug effects / Q000201:enzymology; D002540:Cerebral Cortex / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D050822:Cytokine Receptor gp130; D005333:Fetus; D006131:Growth Inhibitors / Q000378:metabolism / Q000494:pharmacology*; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; D053680:Leukemia Inhibitory Factor Receptor alpha Subunit; D008025:Ligases / Q000235:genetics*; D008222:Lymphokines / Q000378:metabolism / Q000494:pharmacology*; D008562:Membrane Glycoproteins / Q000235:genetics; D009474:Neurons / Q000187:drug effects* / Q000201:enzymology; D009479:Neuropeptides / Q000235:genetics; D018377:Neurotransmitter Agents / Q000096:biosynthesis*; D018847:Opioid Peptides / Q000235:genetics*; D012333:RNA, Messenger / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D018121:Receptors, Cytokine / Q000235:genetics; D053606:Receptors, OSM-LIF; D000097629:Nociceptin
chemical_list: D015703:Antigens, CD; D050822:Cytokine Receptor gp130; D006131:Growth Inhibitors; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; D053680:Leukemia Inhibitory Factor Receptor alpha Subunit; D008025:Ligases; D008222:Lymphokines; D008562:Membrane Glycoproteins; D009479:Neuropeptides; D018377:Neurotransmitter Agents; D018847:Opioid Peptides; D012333:RNA, Messenger; D018121:Receptors, Cytokine; D053606:Receptors, OSM-LIF; D000097629:Nociceptin; C494201:Il6st protein, rat; C507842:Lifr protein, rat