A novel protein interacts with a clock-related protein, rPer1.
Mammalian Per proteins are thought to be important in the mechanism of circadian rhythm. We identified a novel protein PIPS (Per1 interacting protein of the suprachiasmatic nucleus) with the yeast two-hybrid system using PAS domain of rat Per1 (rPer1) as a bait. PIPS is about a 180-kDa protein and expressed mainly in the brain, especially in the hypothalamus including the suprachiasmatic nuclei (SCN). PIPS interacts with mouse Per1 (mPer1) in vitro and in cultured cells transfected with both molecules. Furthermore, it was found that mPer1 translocated PIPS into the nuclei in the cultured cells. Thus, these findings suggest a possibility that PIPS is involved in the feedback loop or output mechanism of circadian rhythm through interacting with Per1 in the SCN.
Matsuki
T
T
Division of Protein Metabolism, Institute for Protein Research, Osaka University, 3-2 Yamada-Oka, Suita, Osaka 565-0871, Japan.
Kiyama
A
A
Kawabuchi
M
M
Okada
M
M
Nagai
K
K
D000595:Amino Acid Sequence / Q000235:genetics; D000818:Animals; D001483:Base Sequence / Q000235:genetics; D001683:Biological Clocks / Q000502:physiology*; D019556:COS Cells; D002451:Cell Compartmentation / Q000235:genetics; D018797:Cell Cycle Proteins; D002467:Cell Nucleus / Q000378:metabolism; D002940:Circadian Rhythm / Q000502:physiology*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000302:isolation & purification; D005246:Feedback / Q000502:physiology; D015723:Gene Library; D007150:Immunohistochemistry; D008297:Male; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D009687:Nuclear Proteins / Q000378:metabolism*; D056950:Period Circadian Proteins; D017434:Protein Structure, Tertiary / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D013493:Suprachiasmatic Nucleus / Q000166:cytology / Q000378:metabolism*; D014178:Translocation, Genetic / Q000502:physiology; D020798:Two-Hybrid System Techniques
D018797:Cell Cycle Proteins; D018076:DNA, Complementary; C443439:Gprasp1 protein, rat; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; C539023:Per1 protein, mouse; C539024:Per1 protein, rat; D056950:Period Circadian Proteins