[Studies on polysaccharide from Akebia trifoliate (I)].
Crude polysaccharide ATB was extracted from the stems of Akebia trifoliate with 5% NaOH. It was then subjected to DEAE-Cellulose column chromatography and purified by Sephadex G-200 chromatography. Finally, a pure polysaccharide ATBB-2 was obtained. The molecular weight of ATBB-2 was estimated to be 2.3 x 10(5). By total acid hydrolysis, the analytical results indicated that ATBB-2 is composed of Rha, Ara, Xyl, Gal, Glc and GalA in a molar ratio of 1.22:1.00:1.10:0.85:0.24:0.82. Methylation analysis, periodate oxidation analysis, Smith degradation, partial acid hydrolysis, 1H and 13CNMR showed that ATBB-2 is a highly branched molecule without a main chain.
Zhang
J S
JS
Shanghai Institute of Materia Medica, Academia Sinica, Shanghai 200031.
Fang
J N
JN
D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D019684:Magnoliopsida / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification*
D004365:Drugs, Chinese Herbal; D011134:Polysaccharides