title: Stability of sufentanil in human plasma samples.
abstract: The stability of sufentanil in human plasma kept under various storage conditions was investigated. Extraction was performed using solid phase extraction with new mixed-mode cation exchange Oasis MCX columns; quantification was carried out using gas chromatography equipped with a mass spectrometry detector. When plasma was left at 4 degrees C in nonsilanized tubes, concentrations of sufentanil decreased significantly during the first hour. In plasma samples kept at -25 degrees C for 8 hours in nonsilanized glass tubes, a significant decrease of sufentanil concentrations was found, with an average loss of 10.1% of the initial concentration. A significant decrease occurred when plasma was kept in silanized glass tubes for 12 hours at -25 degrees C. The current study emphasizes the importance of sampling and storage conditions for an accurate determination of sufentanil concentration in plasma.
authors.affiliation: Department of Clinical Pharmacy, Pharmacokinetics, and Drug Evaluation, Institute of Pharmaceutic and Biologic Sciences, Claude Bernard Lyon 1 University (UCBL), Lyon, France.
authors.forename: C
authors.initials: C
authors.lastname: Dufresne
authors.forename: P
authors.initials: P
authors.lastname: Favetta
authors.forename: C
authors.initials: C
authors.lastname: Paradis
authors.forename: R
authors.initials: R
authors.lastname: Boulieu
mesh_terms: D015760:Alfentanil / Q000097:blood / Q000737:chemistry; D000701:Analgesics, Opioid / Q000097:blood / Q000737:chemistry*; D004349:Drug Packaging; D004355:Drug Stability; D004356:Drug Storage; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D017409:Sufentanil / Q000097:blood / Q000737:chemistry*; D013696:Temperature; D013997:Time Factors
chemical_list: D000701:Analgesics, Opioid; D015760:Alfentanil; D017409:Sufentanil