title: Structural determinants for ligand binding and catalysis of triosephosphate isomerase.
abstract: The crystal structure of leishmania triosephosphate isomerase (TIM) complexed with 2-(N-formyl-N-hydroxy)-aminoethyl phosphonate (IPP) highlights the importance of Asn11 for binding and catalysis. IPP is an analogue of the substrate D-glyceraldehyde-3-phosphate, and it is observed to bind with its aldehyde oxygen in an oxyanion hole formed by ND2 of Asn11 and NE2 of His95. Comparison of the mode of binding of IPP and the transition state analogue phosphoglycolohydroxamate (PGH) suggests that the Glu167 side chain, as well as the triose part of the substrate, adopt different conformations as the catalysed reaction proceeds. Comparison of the TIM-IPP and the TIM-PGH structures with other liganded and unliganded structures also highlights the conformational flexibility of the ligand and the active site, as well as the conserved mode of ligand binding.
authors.affiliation: Biocenter Oulu and Department of Biochemistry, University of Oulu, FIN-90014, Oulu, Finland.
authors.forename: I
authors.initials: I
authors.lastname: Kursula
authors.forename: S
authors.initials: S
authors.lastname: Partanen
authors.forename: A M
authors.initials: AM
authors.lastname: Lambeir
authors.forename: D M
authors.initials: DM
authors.lastname: Antonov
authors.forename: K
authors.initials: K
authors.lastname: Augustyns
authors.forename: R K
authors.initials: RK
authors.lastname: Wierenga
mesh_terms: D000615:Aminoethylphosphonic Acid / Q000031:analogs & derivatives / Q000378:metabolism*; D000818:Animals; D001216:Asparagine / Q000378:metabolism; D002384:Catalysis; D018360:Crystallography, X-Ray; D004791:Enzyme Inhibitors / Q000494:pharmacology; D007891:Leishmania / Q000201:enzymology; D008024:Ligands; D008958:Models, Molecular; D063065:Organophosphonates; D011487:Protein Conformation; D013379:Substrate Specificity; D014305:Triose-Phosphate Isomerase / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: C438753:2-(N-formyl-N-hydroxy)aminoethyl phosphonate; D004791:Enzyme Inhibitors; D008024:Ligands; D063065:Organophosphonates; D001216:Asparagine; D000615:Aminoethylphosphonic Acid; D014305:Triose-Phosphate Isomerase