title: Alternative cell lines for the propagation of lumpy skin disease virus.
abstract: In our Institute lumpy skin disease virus is grown on primary lamb testis cells for isolation, identification and vaccine production. However, the availability of lambs in Kenya has been seriously reduced over the past few years. This has led to an increase in the cost of using primary lamb testis cells. This study was undertaken to investigate other primary cell lines, which are easily available and provide an equivalent or better yield of lumpy skin disease virus. Foetal bovine muscle (FBM) cells were found to be an adequate alternative for lamb testis cells.
authors.affiliation: Kenya Agricultural Research Institute, Nairobi.
authors.forename: Y S
authors.initials: YS
authors.lastname: Binepal
authors.forename: F A
authors.initials: FA
authors.lastname: Ongadi
authors.forename: J C
authors.initials: JC
authors.lastname: Chepkwony
mesh_terms: D000818:Animals; D002417:Cattle; D002460:Cell Line; D002478:Cells, Cultured; D005333:Fetus / Q000166:cytology; D008167:Lumpy skin disease virus / Q000254:growth & development*; D008297:Male; D018482:Muscle, Skeletal / Q000166:cytology* / Q000196:embryology / Q000821:virology; D012756:Sheep; D013737:Testis / Q000166:cytology* / Q000821:virology