Expression of the human sodium/iodide symporter (hNIS) in xenotransplanted human thyroid carcinoma.
The uptake of iodide in thyroid epithelial cells is mediated by the sodium/iodide symporter (NIS). The uptake of iodide is of vital importance for thyroid physiology and is a prerequisite for radioiodine therapy in thyroid cancer. Loss of iodide uptake due to diminished expression of the human NIS (hNIS) is frequently observed in metastasized thyroid cancer. So far, no animal model for the study of radioiodine therapy in thyroid cancer has been available. Strategies to restore iodide uptake in thyroid cancer include the exploration of hNIS gene transfer into hNIS defective thyroid cancer. We have performed a stable transfection of hNIS into the hNIS defective follicular thyroid carcinoma cell line FTC133. Stably transfected colonies exhibited high uptake of Na125I, which could be blocked completely with sodium perchlorate. hNIS transfected FTC133 and non-transfected cell lines injected subcutaneously in nude mice formed tumors after 6 weeks. Iodide uptake in the hNIS transfected tumor was much higher than in non-transfected tumor, but a rapid release of radioactivity from the hNIS transfected tumor was observed. Further studies are necessary to investigate the role of hNIS in relation to other thyroid specific proteins in iodide metabolism in thyroid cancer.
Smit
J W
JW
Department of Endocrinology and Metabolism, Leiden University Medical Center, The Netherlands. j.w.a.smit.endo@lumc.nl
Schröder-van der Elst
J P
JP
Karperien
M
M
Que
I
I
Romijn
J A
JA
van der Heide
D
D
D000818:Animals; D017690:Cell Transplantation; D004195:Disease Models, Animal; D015316:Genetic Therapy; D006801:Humans; D007454:Iodides / Q000378:metabolism*; D007457:Iodine Radioisotopes / Q000378:metabolism / Q000627:therapeutic use; D008099:Liver / Q000378:metabolism; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012588:Scintillation Counting; D013154:Spleen / Q000378:metabolism; D027981:Symporters / Q000235:genetics* / Q000378:metabolism; D013964:Thyroid Neoplasms / Q000378:metabolism / Q000628:therapy*; D013997:Time Factors; D014162:Transfection; D000099209:Sodium-Iodide Symporters
D007454:Iodides; D007457:Iodine Radioisotopes; D012333:RNA, Messenger; D027981:Symporters; D000099209:Sodium-Iodide Symporters