title: A hemicellulose B fraction from grape skin (Vitis vinifera, Palomino variety).
abstract: The structure of a hemicellulose B fraction (B-1) isolated from grape skins (Vitis vinifera) of the Palomino variety has been studied by methylation analysis, (1)H NMR and (13)C NMR spectroscopy, and partial acid hydrolysis. Hemicellulose B-1 appeared to be homogeneous by gel filtration with a weight-average molecular weight of 22 600. This polysaccharide is a linear xyloglucan chain composed of xylopyranosyl and glucopyranosyl residues linked by beta-(1-->4) glycosidic bonds. Attached to this backbone, 4-O-methyl-D-glucuronopyranosyl acid, D-glucopyranosyl, and L-fucopyranosyl residues occur at position 2 in a ratio of one residue for every five units of xylose in the main chain, with D-xylopyranosyl residues attached at position 6 of glucose units in a ratio of one residue for every two glucose-derived moieties in the main chain.
authors.affiliation: Departamento de Química Orgánica, Facultad de Ciencias, Universidad de Cádiz, Apartado 40, 11510-Puerto Real, Cádiz, Spain. josemanuel.igartuburu@uca.es
authors.forename: J M
authors.initials: JM
authors.lastname: Igartuburu
authors.forename: E
authors.initials: E
authors.lastname: Pando
authors.forename: F
authors.initials: F
authors.lastname: Rodríguez Luis
authors.forename: A
authors.initials: A
authors.lastname: Gil-Serrano
mesh_terms: D002240:Carbohydrate Sequence; D002482:Cellulose / Q000737:chemistry / Q000302:isolation & purification*; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D002854:Chromatography, Paper; D004043:Dietary Fiber / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008745:Methylation; D008969:Molecular Sequence Data; D015394:Molecular Structure; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D012639:Seeds / Q000737:chemistry; D013030:Spain; D013055:Spectrophotometry, Infrared; D027843:Vitis / Q000737:chemistry*
chemical_list: D004043:Dietary Fiber; D011134:Polysaccharides; C438402:hemicellulose B; D002482:Cellulose