Catecholamines in murine bone marrow derived mast cells.
Cultured murine bone marrow derived mast cells (BMMC) were found to store high levels of dopamine (3753+/-844 pg/10(7) cells) and occasionally produce norepinephrine and epinephrine. The catecholamine synthesis inhibitor, alpha-methyl-para-tyrosine, decreased intracellular catecholamine concentrations, and activation with ionomycin stimulated dopamine release. Neither dopaminergic receptor antagonists nor exogenous dopamine < or =10 microM affected IL-3-induced cell proliferation. High exogenous dopamine (20-100 microM) decreased proliferation and increased apoptosis, and the anti-oxidant ascorbic acid prevented these effects. Increased expression of the anti-apoptotic factor Bcl-2 or loss of pro-apoptotic Bax expression attenuated dopamine-induced apoptosis, suggesting the apoptosis proceeds through a mitochondrial pathway.
Freeman
J G
JG
Department of Biology, Virginia Commonwealth University, Richmond, VA 23284-2012, USA.
Ryan
J J
JJ
Shelburne
C P
CP
Bailey
D P
DP
Bouton
L A
LA
Narasimhachari
N
N
Domen
J
J
Siméon
N
N
Couderc
F
F
Stewart
J K
JK
D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000276:immunology; D001552:Benzazepines / Q000494:pharmacology; D001713:Biphenyl Compounds / Q000494:pharmacology; D001854:Bone Marrow Cells / Q000166:cytology* / Q000378:metabolism*; D002395:Catecholamines / Q000096:biosynthesis*; D002455:Cell Division / Q000187:drug effects / Q000276:immunology; D002470:Cell Survival / Q000187:drug effects / Q000276:immunology; D002478:Cells, Cultured; D004298:Dopamine / Q000096:biosynthesis; D018492:Dopamine Antagonists / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004837:Epinephrine / Q000096:biosynthesis; D007377:Interleukin-3 / Q000494:pharmacology; D015759:Ionomycin / Q000494:pharmacology; D007476:Ionophores / Q000494:pharmacology; D008407:Mast Cells / Q000166:cytology* / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D009638:Norepinephrine / Q000096:biosynthesis; D010084:Oxidation-Reduction; D010879:Piperazines / Q000494:pharmacology; D011518:Proto-Oncogene Proteins / Q000235:genetics; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics; D020891:Raclopride / Q000494:pharmacology; D019805:alpha-Methyltyrosine / Q000494:pharmacology; D051028:bcl-2-Associated X Protein
C494827:Bax protein, mouse; D001552:Benzazepines; D001713:Biphenyl Compounds; D002395:Catecholamines; D018492:Dopamine Antagonists; D004791:Enzyme Inhibitors; D007377:Interleukin-3; D007476:Ionophores; D010879:Piperazines; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; C105771:GR 103691; D020891:Raclopride; D015759:Ionomycin; D019805:alpha-Methyltyrosine; D004298:Dopamine; D009638:Norepinephrine; D004837:Epinephrine