Amino acid substitution within the VP7 protein of G2 rotavirus strains associated with failure to serotype.
Rotavirus strains collected in the United Kingdom during the 1995-1996 season and genotyped as G2 by reverse transcription-PCR failed to serotype in enzyme-linked immunosorbent assays using three different G2-specific monoclonal antibodies. The deduced amino acid sequences of the antigenic regions A (amino acids 87 to 101), B (amino acids 142 to 152), and C (amino acids 208 to 221) of VP7 revealed that a substitution at position 96 (Asp-->Asn) correlated with the change in ability to serotype these G2 strains.
Gómara
M I
MI
Clinical Microbiology and Public Health Laboratory, Addenbrooke's Hospital, Cambridge CB2 2QW, United Kingdom. mirren.iturriza-gomara@addenbrookes.nhs.uk
Cubitt
D
D
Desselberger
U
U
Gray
J
J
D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000911:Antibodies, Monoclonal / Q000276:immunology; D000956:Antigens, Viral; D002213:Capsid / Q000737:chemistry* / Q000235:genetics* / Q000276:immunology; D036022:Capsid Proteins; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012401:Rotavirus / Q000145:classification* / Q000235:genetics; D012400:Rotavirus Infections / Q000821:virology; D012703:Serotyping
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D036022:Capsid Proteins; C050779:VP7 protein, Rotavirus