Effect of the central disulfide bond on the unfolding behavior of elongation factor Ts homodimer from Thermus thermophilus.
Functionally active elongation factor Ts (EF-Ts) from Thermus thermophilus forms a homodimer. The dimerization interface of EF-Ts is composed of two antiparallel beta-sheets that can be connected by an intermolecular disulfide bond. The stability of EF-Ts from T. thermophilus in the presence and absence of the intermolecular disulfide bond was studied by differential scanning calorimetry and circular dichroism. The ratio of the van't Hoff and calorimetric enthalpies, delta H(vH)/delta H(cal), indicates that EF-Ts undergoes thermal unfolding as a dimer independently of the presence or absence of the disulfide bond. This can be concluded from (1) the presence of residual secondary structure above the thermal transition temperature, (2) the absence of concentration dependence, which would be expected for dissociation of the dimer prior to unfolding of the monomers, and (3) a relatively low heat capacity change (delta Cp) upon unfolding. The retained dimeric structure of the thermally denatured state allowed for the determination of the effect of the intermolecular disulfide bond on the conformational stability of EF-Ts, which is deltadelta G(S-S,SH HS) = 10.5 kJ/mol per monomer at 72.5 degrees C. The possible physiological implications of the dimeric EF-Ts structure and of the intersubunit disulfide bond for the extreme conformational stability of proteins in thermophiles are discussed.
Sedlák
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Department of Biochemistry, Faculty of Science P. J. Safárik University, Kosice, Slovakia.
Valusová
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Nesper-Brock
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Antalík
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Sprinzl
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D001426:Bacterial Proteins / Q000737:chemistry*; D002152:Calorimetry, Differential Scanning; D019281:Dimerization; D004220:Disulfides / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008958:Models, Molecular; D010445:Peptide Elongation Factors / Q000737:chemistry* / Q000378:metabolism; D010472:Perchlorates / Q000737:chemistry; D011489:Protein Denaturation; D017510:Protein Folding; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D017670:Sodium Compounds / Q000737:chemistry; D013696:Temperature; D016963:Thermus thermophilus / Q000737:chemistry*
D001426:Bacterial Proteins; D004220:Disulfides; D010445:Peptide Elongation Factors; D010472:Perchlorates; D017670:Sodium Compounds; C023512:elongation factor Ts; C031068:sodium perchlorate