Rapid chiral on-chip separation with simplified amperometric detection.
The enantiomers of adrenaline, noradrenaline, ephedrine and pseudoephedrine were separated by capillary electrophoresis on a micromachined device. Detection was carried out with a new two-electrode amperometric detector, eliminating the need for individual counter and reference electrodes. Separation of the isomers was achieved by employing carboxymethyl-beta-cyclodextrin as chiral selector in the buffer, partly with the additional inclusion of the crown ether 18-crown-6. Plate numbers of up to 20,000, chiral resolutions of 2.5 and detection limits of the order of 10(-7) M were achieved. All separations were completed in less then 3 min.
Schwarz
M A
MA
The University of Basel, Department of Chemistry, Switzerland.
Hauser
P C
PC
D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods*; D004809:Ephedrine / Q000032:analysis; D004837:Epinephrine / Q000032:analysis; D008904:Miniaturization; D009638:Norepinephrine / Q000032:analysis; D013237:Stereoisomerism
D004809:Ephedrine; D009638:Norepinephrine; D004837:Epinephrine