Immunohistochemical analysis of the role of angiogenic status in the vasculature of peritoneal endometriosis.
OBJECTIVE
To investigate the angiogenic status of the vasculature in peritoneal endometriosis based on macroscopic appearance.


METHODS
Prospective study.


METHODS
University hospital.


METHODS
Patients with peritoneal endometriosis.


METHODS
During laparoscopy, 25 samples of predominantly red peritoneal lesions and 27 samples of predominantly black peritoneal lesions were collected from a total of 31 patients with endometriosis. Eutopic endometrium from 25 patients with endometriosis was collected by curettage during laparoscopy or just after surgery.


METHODS
A proliferating endothelial cell index (PCI) was determined by calculating the percentage of microvessels that contained proliferating endothelial cells. A vessel maturation index (VMI) was determined by calculating the percentage of microvessels showing colocalization of CD34- and alpha-SMA-positive staining.


RESULTS
Peritoneal endometriotic tissues had extremely low or null PCI. The VMI of red peritoneal lesions was significantly lower than that of black ones. Vessel maturation index of red lesions was higher that that of proliferative eutopic endometrium and similar to that of secretory eutopic endometrium.


CONCLUSIONS
Compared with the case of black peritoneal lesions, red lesions had a much higher fraction of immature vessels.
Matsuzaki
S
S
Department of Obstetrics and Gynecology, Tohoku University School of Medicine, Sendai, Japan. sachikoMA@aol.com
Canis
M
M
Murakami
T
T
Dechelotte
P
P
Bruhat
M A
MA
Okamura
K
K
D000199:Actins / Q000378:metabolism; D000328:Adult; D018952:Antigens, CD34 / Q000378:metabolism; D001808:Blood Vessels / Q000378:metabolism / Q000473:pathology; D002455:Cell Division; D004715:Endometriosis / Q000150:complications*; D004730:Endothelium, Vascular / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D009131:Muscle, Smooth, Vascular / Q000378:metabolism; D009389:Neovascularization, Pathologic / Q000209:etiology* / Q000473:pathology; D010532:Peritoneal Diseases / Q000150:complications*; D011247:Pregnancy; D011446:Prospective Studies
D000199:Actins; D018952:Antigens, CD34