Immunohistochemical determination of estrogen and progesterone receptors and DNA flow cytometry in endometrial cancer.
Estrogen and progesterone receptor contents (ER, PR) were assessed by an immunohistochemical method and DNA ploidy and S-phase by flow cytometry in frozen endometrial cancer tissue sections from 39 cases. Comparison of the immunohistochemical and cytosol assays showed 81% and 84% concordance in ER and PR contents, respectively. An aneuploid DNA pattern was identified in 30% and a high S-phase fraction was found in 33% of 36 specimens studied. Negative ER status was associated with aneuploid and high S-phase fraction. A similar association was found between PR status and high S-phase fraction. Combined analysis of immunohistochemical receptor status and DNA flow cytometry in the same sample makes it possible to identify two strong predictive factors in endometrial adenocarcinoma.
Heinonen
P.K.
PK
Departments of Clinical and Biomedical Sciences, University of Tampere, and Department of Obstetrics and Gynecology, University Hospital, Tampere, Finland.
Isola
J.
J
Kuoppala
T.
T