Leukemia inhibitory factor induces nociceptin mRNA in cultured rat cortical neurons.
The effects of leukemia inhibitory factor (LIF) on the expression of neurotransmitter synthetase and neuropeptide mRNAs in cultured rat cortical neurons were examined by reverse transcription-polymerase chain reaction. Nociceptin mRNA expression was increased by treatment with 20 or 80 ng/ml LIF for 24 h, but choline acetyl transferase, glutamic acid decarboxylase, enkephalin, dynorphin, substance P, somatostatin and galanin mRNA expression were not altered by LIF. These observations indicated a specific effect of LIF on nociceptin gene regulation in cultured cortical neurons.
Minami
M
M
Department of Molecular Pharmacology, Faculty of Pharmaceutical Sciences, Kyoto University, 606-8501, Kyoto, Japan.
Yamakuni
H
H
Ohtani
Y
Y
Okada
M
M
Nakamura
J
J
Satoh
M
M
D000375:Aging / Q000235:genetics; D000818:Animals; D015703:Antigens, CD / Q000235:genetics; D002478:Cells, Cultured / Q000187:drug effects / Q000201:enzymology; D002540:Cerebral Cortex / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D050822:Cytokine Receptor gp130; D005333:Fetus; D006131:Growth Inhibitors / Q000378:metabolism / Q000494:pharmacology*; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; D053680:Leukemia Inhibitory Factor Receptor alpha Subunit; D008025:Ligases / Q000235:genetics*; D008222:Lymphokines / Q000378:metabolism / Q000494:pharmacology*; D008562:Membrane Glycoproteins / Q000235:genetics; D009474:Neurons / Q000187:drug effects* / Q000201:enzymology; D009479:Neuropeptides / Q000235:genetics; D018377:Neurotransmitter Agents / Q000096:biosynthesis*; D018847:Opioid Peptides / Q000235:genetics*; D012333:RNA, Messenger / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D018121:Receptors, Cytokine / Q000235:genetics; D053606:Receptors, OSM-LIF; D000097629:Nociceptin
D015703:Antigens, CD; D050822:Cytokine Receptor gp130; D006131:Growth Inhibitors; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; D053680:Leukemia Inhibitory Factor Receptor alpha Subunit; D008025:Ligases; D008222:Lymphokines; D008562:Membrane Glycoproteins; D009479:Neuropeptides; D018377:Neurotransmitter Agents; D018847:Opioid Peptides; D012333:RNA, Messenger; D018121:Receptors, Cytokine; D053606:Receptors, OSM-LIF; D000097629:Nociceptin; C494201:Il6st protein, rat; C507842:Lifr protein, rat