Isomerization of a uniquely designed amplicon during herpes simplex virus-mediated replication.
Herpes simplex virus (HSV) type 1 DNA isomerization was studied using a uniquely designed amplicon that mimics the viral genomic structure. The results revealed that amplicon concatemers frequently contain adjacent amplicon units with their segments in opposed orientations. These unusual concatemers were generated through homologous recombination, which does not require HSV DNA as the source of homology.
Wang
H
H
Center for Cell and Gene Therapy, Baylor College of Medicine, Houston, Texas 77030, USA.
Fu
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X
Zhang
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X
D000818:Animals; D015139:Blotting, Southern; D002460:Cell Line; D006224:Cricetinae; D004261:DNA Replication; D004279:DNA, Viral / Q000737:chemistry; D005784:Gene Amplification; D011995:Recombination, Genetic; D018139:Simplexvirus / Q000235:genetics*; D014779:Virus Replication
D004279:DNA, Viral