Cloning and characterization of KLHL5, a novel human gene encoding a kelch-related protein with a BTB domain.
Most kelch family proteins contain two conserved domains, the BTB domain and the kelch repeat domain. Here we describe the cloning and characterization of a novel human KLHL5 gene. The 3488 bp cDNA encodes a kelch family protein homologous to the Drosophila kelch protein. It also contains the two conserved domains. Northern blot analysis reveals a single transcript. It is abundantly expressed in ovary, adrenal gland, and thyroid and less abundantly expressed in trachea, prostate, testis, lymph node, and spinal cord. KLHL5 was mapped to 4p13-4p15.1.
Wang
S
S
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, Shanghai, People's Republic of China.
Zhou
Z
Z
Ying
K
K
Tang
R
R
Huang
Y
Y
Wu
C
C
Xie
Y
Y
Mao
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics*; D002894:Chromosomes, Human, Pair 4 / Q000235:genetics; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004330:Drosophila; D029721:Drosophila Proteins / Q000737:chemistry; D015723:Gene Library; D006801:Humans; D008840:Microfilament Proteins; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D002352:Carrier Proteins; D018076:DNA, Complementary; D029721:Drosophila Proteins; C450047:KLHL5 protein, human; D008840:Microfilament Proteins; C080353:kel protein, Drosophila