title: The pattern of gene expression in mouse Gr-1(+) myeloid progenitor cells.
abstract: To understand the pattern of gene expression in mouse myeloid progenitor cells, we carried out a genome-wide analysis of gene expression in mouse bone marrow Gr-1(+) cells using SAGE and GLGI techniques. We identified 22,033 unique SAGE tags with quantitative information from 73,869 collected SAGE tags. Among these unique tags, 64% match known sequences, including many genes important for myeloid differentiation, and 36% have no matches to known sequences and are likely to represent novel genes. We compared the expression of mouse Gr-1(+) and human CD15(+) myeloid progenitor cells and showed that the pattern of gene expression of these two cell populations had some similarities. We also compared the expression of mouse Gr-1(+) myeloid progenitor cells with that of mouse brain tissue and found a highly tissue-specific manner of gene expression in these two samples. Our data provide a basis for studying altered gene expression in myeloid disorders using mouse models.
authors.affiliation: Department of Medicine, University of Chicago, 5841 S. Maryland, MC2115, Chicago, Illinois 60637, USA.
authors.forename: J
authors.initials: J
authors.lastname: Chen
authors.forename: D A
authors.initials: DA
authors.lastname: Rowley
authors.forename: T
authors.initials: T
authors.lastname: Clark
authors.forename: S
authors.initials: S
authors.lastname: Lee
authors.forename: G
authors.initials: G
authors.lastname: Zhou
authors.forename: C
authors.initials: C
authors.lastname: Beck
authors.forename: J D
authors.initials: JD
authors.lastname: Rowley
authors.forename: S M
authors.initials: SM
authors.lastname: Wang
mesh_terms: D000818:Animals; D000943:Antigens, Differentiation / Q000032:analysis; D001921:Brain / Q000378:metabolism; D002454:Cell Differentiation; D018076:DNA, Complementary; D020224:Expressed Sequence Tags; D005260:Female; D015870:Gene Expression; D020869:Gene Expression Profiling; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D023461:Myeloid Progenitor Cells / Q000378:metabolism*
chemical_list: D000943:Antigens, Differentiation; D018076:DNA, Complementary