title: The role of ICAM-1 molecule in the migration of Langerhans cells in the skin and regional lymph node.
abstract: ICAM-1 (CD54) plays an important role in the cell-cell interaction and migration of leukocytes. Previous studies have shown that ICAM-1 is involved in inflammatory reactions and that a defect in ICAM-1 gene inhibits allergic contact hypersensitivity. This study indicates that the migration of hapten presenting Langerhans cells into the regional lymph nodes was significantly reduced in ICAM-1-deficient mice compared to wild-type C57BL/6 mice. The reduced number of dendritic cells in regional lymph nodes did not result from abnormal migration of Langerhans cells into the skin of ICAM-1-deficient mice. The concentration and distribution of Langerhans cells in the naïve skin of ICAM-1-deficient mice was equal to that of wild-type mice. Following hapten sensitization, Langerhans cell migration out of the skin and recruitment of fresh Langerhans cells back to the epidermis was not affected in ICAM-1-deficient mice. Further experiments demonstrated that ICAM-1 deficiency on lymphatic endothelium rather than on dendritic cells was responsible for the reduced migration of Langerhans cells into draining lymph nodes. This study indicates that ICAM-1 regulates the migration of dendritic cells into regional lymph nodes but not into or out of the skin.
authors.affiliation: Department of Dermatology, University of Alabama at Birmingham, Birmingham 35294, USA. xuhui@uab.edu
authors.forename: H
authors.initials: H
authors.lastname: Xu
authors.forename: H
authors.initials: H
authors.lastname: Guan
authors.forename: G
authors.initials: G
authors.lastname: Zu
authors.forename: D
authors.initials: D
authors.lastname: Bullard
authors.forename: J
authors.initials: J
authors.lastname: Hanson
authors.forename: M
authors.initials: M
authors.lastname: Slater
authors.forename: C A
authors.initials: CA
authors.lastname: Elmets
mesh_terms: D000818:Animals; D002465:Cell Movement; D003713:Dendritic Cells / Q000502:physiology; D018799:Intercellular Adhesion Molecule-1 / Q000502:physiology*; D007801:Langerhans Cells / Q000502:physiology*; D008198:Lymph Nodes / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D012867:Skin / Q000276:immunology*
chemical_list: D018799:Intercellular Adhesion Molecule-1