title: Determination of nandrolone and metabolites in urine samples from sedentary persons and sportsmen.
abstract: Metabolites of nandrolone were determined in the urine of several sportsmen, sedentary and post-menopausal women by capillary gas chromatography-mass spectrometry quadrupole (GC-MS) and capillary gas chromatography mass-mass spectrometry ion trap (GC-MS-MS) methods. The method employed was GC-EI-MS with 17alpha-methyltestosterone as internal standard with ethyl ether extraction prior to selected ion monitoring of the bis(trimethylsilyl) ethers at ion masses m/z 405 and 420 for the nandrolone metabolites, and 418 and 403 for nandrolone derivative. Recovery for nandrolone, 19-norandrosterone (19-NA) and 19-noretiocholanolone (19-NE) was 97.20, 94.17 and 95.54%, respectively. Detection limits for nandrolone, 19-NA and 19-NE were 0.03, 0.01 and 0.06 ng/ml. Metabolites of nandrolone (19-NA and 19-NE) were found in 12.5% (n = 40) of sportsmen and 40% (n = 10) of post-menopausal women.
authors.affiliation: Department of Analytical Chemistry and Electrochemistry, Faculty of Sciences, Extremadura University, Badajoz, Spain.
authors.forename: A M
authors.initials: AM
authors.lastname: Galán Martín
authors.forename: J I
authors.initials: JI
authors.lastname: Mariño
authors.forename: M P
authors.initials: MP
authors.lastname: García de Tiedra
authors.forename: J J
authors.initials: JJ
authors.lastname: Marabé
authors.forename: M J
authors.initials: MJ
authors.lastname: Caballero Loscos
authors.forename: M M
authors.initials: MM
authors.lastname: Mariño
mesh_terms: D002138:Calibration; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D009277:Nandrolone / Q000652:urine*; D012680:Sensitivity and Specificity; D013177:Sports
chemical_list: D009277:Nandrolone