Differential expression of protein kinase C betaI (PKCbetaI) but not PKCalpha and PKCbetaII in the suprachiasmatic nucleus of selected house mouse lines, and the relationship to arginine-vasopressin.
The functional significance of the suprachiasmatic nucleus (SCN) in circadian rhythm control of mammals has been well documented. The role of protein phosphorylation mediated by protein kinase C (PKC), however, is not well known. We report the immunocytochemical localization of three Ca(2+)-dependent PKC isoforms (alpha, betaI, betaII) within the SCN of selected house mouse lines that differ in behavioral circadian rhythm parameters. Optical density measurements revealed that the adult mice selected for low levels of nest-building behavior (small nest-builders) had more than threefold higher PKCbetaI immunostaining in the SCN than the mice selected for high levels of nest-building behavior (big nest-builders). A similar twofold difference between the adult small and big nest-builders was observed for the number of PKCbetaI-containing cells in the SCN. The non-selected control lines were intermediate. Ten-day-old pups revealed similar differences in PKCbetaI immunostaining in the SCN between the small and big nest-builders. PKCalpha and PKCbetaII immunostaining in the SCN was not different among the lines. PKCbetaI immunostaining was not different among the selected lines in the lateroanterior hypothalamic nucleus (LA) and the cornu ammonis field 1 (CA1) of the dorsal hippocampus and confirms the specificity of the difference in PKCbetaI immunostaining in the SCN among the selected lines. The significance of these findings is discussed in the context of differences among the lines in arginine-vasopressin (AVP) and light-induced Fos expression in the SCN, behavioral phase-delay responses to 15-min light pulses in constant darkness, and measures of the strength of the circadian activity rhythm expressed.
Bult
A
A
Alaskan Basic Neuroscience Program, Institute of Arctic Biology, University of Alaska Fairbanks, P.O. Box 757000, Fairbanks, AK 99775-7000, USA. ffab@uab.edu
Kobylk
M E
ME
Van der Zee
E A
EA
D000375:Aging / Q000502:physiology; D000818:Animals; D001127:Arginine Vasopressin / Q000378:metabolism*; D002452:Cell Count; D002940:Circadian Rhythm / Q000502:physiology; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000378:metabolism*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains / Q000033:anatomy & histology / Q000378:metabolism*; D009043:Motor Activity / Q000502:physiology; D009425:Nesting Behavior / Q000502:physiology; D010775:Photic Stimulation; D017729:Presynaptic Terminals / Q000378:metabolism / Q000648:ultrastructure; D020033:Protein Isoforms / Q000378:metabolism; D011493:Protein Kinase C / Q000378:metabolism*; D064546:Protein Kinase C beta; D051571:Protein Kinase C-alpha; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism; D011930:Reaction Time / Q000502:physiology; D015398:Signal Transduction / Q000502:physiology; D013493:Suprachiasmatic Nucleus / Q000166:cytology / Q000201:enzymology* / Q000378:metabolism
D007527:Isoenzymes; D020033:Protein Isoforms; D016760:Proto-Oncogene Proteins c-fos; D001127:Arginine Vasopressin; C496630:Prkca protein, mouse; D011493:Protein Kinase C; D064546:Protein Kinase C beta; D051571:Protein Kinase C-alpha