Differential expression of activin subunits, activin receptors and follistatin genes in bovine oocytes and preimplantation embryos.
The presence of inhibin subunits (alpha, betaA and betaB), activin binding protein follistatin and activin receptors (ActR-I, ActR-IB, ActR-II and ActR-IIB) mRNA transcripts in bovine oocytes and preimplantation embryos was examined by reverse transcription-polymerase chain reaction. Immature and matured oocytes, zygotes, 2-cell, 3-4-cell, 5-8-cell, 9-16-cell embryos, morulae, blastocysts and hatched blastocysts were produced by in vitro maturation, fertilization and culture for this study. Activin betaA subunit and follistatin transcripts were detected in all of the stages tested. However, alpha subunit transcripts were detected only in oocytes (immature and matured), blastocysts and hatched blastocysts, and no betaB subunit transcripts were found in any of the stages. Whereas ActR-I transcripts were detectable in all stages, ActR-IB transcripts were not detectable in oocytes or embryos except in matured oocytes. Activin type II receptors (ActR-II and ActR-IIB) transcripts were detectable in oocytes and embryos except for ActR-IIB, which was not found in the 5-8-cell stage embryos. The results suggest that bovine embryos may produce activin A (homodimer of the betaA subunits) at least from zygotes to morulae. Furthermore, bovine oocytes and embryos express the activin receptors mainly by the combination of ActR-I and either ActR-II or ActR-IIB.
Yoshioka
K
K
Laboratories of Theriogenology, National Institute ofAnimal Health, Tsukuba, Ibaraki, Japan. kojiyos@niah.affrc.go.jp
Takata
M
M
Taniguchi
T
T
Yamanaka
H
H
Sekikawa
K
K
D029404:Activin Receptors; D030201:Activin Receptors, Type I; D030301:Activin Receptors, Type II; D028341:Activins; D000818:Animals; D001755:Blastocyst / Q000378:metabolism*; D002417:Cattle; D019281:Dimerization; D004622:Embryo, Mammalian / Q000378:metabolism; D047108:Embryonic Development; D005260:Female; D038681:Follistatin; D015870:Gene Expression; D006023:Glycoproteins / Q000235:genetics*; D007265:Inhibins / Q000235:genetics*; D009028:Morula / Q000378:metabolism; D009865:Oocytes / Q000378:metabolism*; D011247:Pregnancy; D021122:Protein Subunits; D012333:RNA, Messenger / Q000032:analysis; D017978:Receptors, Growth Factor / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015053:Zygote / Q000378:metabolism
D038681:Follistatin; D006023:Glycoproteins; D021122:Protein Subunits; D012333:RNA, Messenger; D017978:Receptors, Growth Factor; D028341:Activins; D007265:Inhibins; D029404:Activin Receptors; D030201:Activin Receptors, Type I; D030301:Activin Receptors, Type II