title: Characterization of RNase P holoenzymes from Methanococcus jannaschii and Methanothermobacter thermoautotrophicus.
abstract: The partial purification and basic biochemical characterization of the RNase P holoenzymes of two species of methanogenic Archaea, Methanothermobacter thermoautotrophicus (previously Methanobacterium thermoautotrophicum strain deltaH) and Methanococcus jannaschii, are described. The properties of these enzymes, particularly buoyant density in Cs2SO4 and recent information about the subunit composition of the archaeal enzymes, suggest that RNase P enzymes in Archaea are much more alike than earlier studies in Sulfolobus acidocaldarius and Haloferax volcanii suggested.
authors.affiliation: Department of Microbiology, North Carolina State University, Raleigh 27695-7615, USA.
authors.forename: A J
authors.initials: AJ
authors.lastname: Andrews
authors.forename: T A
authors.initials: TA
authors.lastname: Hall
authors.forename: J W
authors.initials: JW
authors.lastname: Brown
mesh_terms: D002499:Centrifugation, Density Gradient; D004722:Endoribonucleases / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D017013:Methanobacteriaceae / Q000201:enzymology*; D017017:Methanococcus / Q000201:enzymology*; D008970:Molecular Weight; D016337:RNA, Catalytic / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D043262:Ribonuclease P
chemical_list: D016337:RNA, Catalytic; D004722:Endoribonucleases; D043262:Ribonuclease P