Positively charged termini of the L2 minor capsid protein are necessary for papillomavirus infection.
Coexpression of bovine papillomavirus L1 with L2 mutants lacking either eight N-terminal or nine C-terminal amino acids that encode positively charged domains resulted in wild-type levels of viral genome encapsidation. Despite wild-type binding to the cell surface, the resulting virions were noninfectious. An L2 mutant encoding a scrambled version of the nine C-terminal residues restored infectivity, in contrast to an L2 mutant encoding a scrambled version of the N-terminal residues.
Roden
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RB
Department of Pathology, The Johns Hopkins University, Baltimore, Maryland 21205-2196, USA. roden@jhmi.edu
Day
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PM
Bronzo
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BK
Yutzy
W H
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Yang
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Y
Lowy
D R
DR
Schiller
J T
JT
D000818:Animals; D052688:Bovine papillomavirus 1 / Q000502:physiology*; D002213:Capsid / Q000737:chemistry / Q000502:physiology*; D036022:Capsid Proteins; D002460:Cell Line; D006224:Cricetinae; D009363:Neoplasm Proteins / Q000032:analysis; D009687:Nuclear Proteins; D014157:Transcription Factors / Q000032:analysis; D025521:Tumor Suppressor Proteins; D014771:Virion / Q000502:physiology; D019065:Virus Assembly
D036022:Capsid Proteins; C085217:L1 protein, Bovine papillomavirus; C085218:L2 protein, Bovine papillomavirus; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D014157:Transcription Factors; D025521:Tumor Suppressor Proteins