title: Expression of an isoform of the testis-specific estrogen sulfotransferase in the murine placenta during the late gestational period.
abstract: Cytosolic sulfotransferases play essential roles in regulating the activities and transfer of steroids. To evaluate their biological significance in the murine uterus and placenta during the course of gestation, we determined their activities with several steroids as substrates. Activated estrogen sulfotransferase (EST) was found in the placenta and uterus during the late gestational period. Reverse-transcribed cDNA of murine placental EST (mpEST) was isolated from mouse placenta at 18 days of gestation and its expression in the tissue coincided with a change in its enzyme activity. The open-reading frame of mpEST encodes a protein composed of 296 amino acids with a predicted molecular mass of 35.5 kDa and was revealed to be an isoform of the murine testis-specific EST gene (99.7%). Also, the amino acid sequence of mpEST showed 49.6 and 77.9% homology with human placental and endometrial EST, respectively, showing that it corresponds to human endometrial EST. COS-7 cells transfected with mpEST exhibited sulfotransferase activity with the phenolic hydroxy groups of steroids and artificial substrates. The best acceptor substrate was estrogen.
authors.affiliation: Department of Obstetrics and Gynecology, School of Medicine, Keio University, 35 Shinano-machi, Shinjuku-ku, Tokyo, 160-8582, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Takehara
authors.forename: K
authors.initials: K
authors.lastname: Kubushiro
authors.forename: Y
authors.initials: Y
authors.lastname: Iwamori
authors.forename: K
authors.initials: K
authors.lastname: Tsukazaki
authors.forename: S
authors.initials: S
authors.lastname: Nozawa
authors.forename: M
authors.initials: M
authors.lastname: Iwamori
mesh_terms: D000818:Animals; D001483:Base Sequence; D019556:COS Cells; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000302:isolation & purification; D004789:Enzyme Activation; D005260:Female; D015870:Gene Expression; D005865:Gestational Age; D007527:Isoenzymes / Q000096:biosynthesis / Q000235:genetics; D008297:Male; D051379:Mice; D008812:Mice, Hairless; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010920:Placenta / Q000201:enzymology*; D011247:Pregnancy; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D015238:Sulfotransferases / Q000096:biosynthesis* / Q000235:genetics; D013737:Testis / Q000201:enzymology*; D014162:Transfection; D014599:Uterus / Q000201:enzymology
chemical_list: D018076:DNA, Complementary; D007527:Isoenzymes; D015238:Sulfotransferases; C020122:estrone sulfotransferase