Presynaptic imaging techniques.
Understanding the detailed molecular events that support chemical synaptic transmission requires high-resolution methods that provide quantitative information combined with molecular specificity. In recent years, many new technological approaches, including genetically encoded fluorescent indicators, ultra-thin sectioning, and live-cell imaging have been brought to bear on understanding the cell biology and physiology of presynaptic terminals.
Ryan
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Department of Biochemistry, The Weill Medical College of Cornell University, New York, NY 10021, USA. taryan@med.cornell.edu
D000818:Animals; D005456:Fluorescent Dyes / Q000032:analysis; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D009474:Neurons / Q000737:chemistry / Q000502:physiology; D017729:Presynaptic Terminals / Q000737:chemistry* / Q000502:physiology; D011726:Pyridinium Compounds / Q000032:analysis; D000644:Quaternary Ammonium Compounds / Q000032:analysis; D009435:Synaptic Transmission / Q000502:physiology; D013572:Synaptic Vesicles / Q000737:chemistry / Q000502:physiology
C073804:FM1 43; D005456:Fluorescent Dyes; D011726:Pyridinium Compounds; D000644:Quaternary Ammonium Compounds