Characterization of monoclonal antibodies directed against the bovine herpesvirus-1 glycoprotein E and use for the differentiation between vaccinated and infected animals.
A panel of seven monoclonal antibodies (MAbs) directed against the bovine herpesvirus-1 (BHV-1) glycoprotein E (gE) was obtained. For that purpose, mice were either tolerized to BHV-1 gE-negative virus and then immunized with wild type BHV-1 or immunized with plasmid DNA expressing the gE and gI glycoproteins. The MAbs were characterized by their reactivity with the gE protein or the gE/gI complex and by competition experiments. Results showed that the MAbs were directed against three antigenic domains, two located on the gE glycoprotein and one on the gE/gI complex. Blocking experiments were performed with sera from experimentally vaccinated and infected cattle. A competition was observed between gE-positive bovine sera and six of the seven MAbs. The bovine sera thus recognized two of the three antigenic sites. Field sera were then tested in blocking enzyme-linked immunosorbent assay using one horseradish peroxidase-conjugated MAb. A specificity of 98.2% and a sensitivity of 98.2% compared to the commercially available test were observed.
Letellier
C
C
Veterinary and Agrochemical Research Centre, 1180 Brussels, Belgium. calet@var.fgov.be
Delangre
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A
De Smet
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A
Kerkhofs
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P
D000818:Animals; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology* / Q000517:prevention & control; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary; D005260:Female; D006566:Herpesviridae Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D007242:Herpesvirus 1, Bovine / Q000276:immunology*; D007106:Immune Sera; D051379:Mice; D008807:Mice, Inbred BALB C; D012680:Sensitivity and Specificity; D014611:Vaccination / Q000662:veterinary; D014759:Viral Envelope Proteins / Q000276:immunology*; D014764:Viral Proteins; D014765:Viral Vaccines / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D007106:Immune Sera; D014759:Viral Envelope Proteins; D014764:Viral Proteins; D014765:Viral Vaccines; C046998:bovine herpesvirus type-1 glycoproteins