Fluorescence nanotomography using resonance energy transfer: demonstration with a protein-sugar complex.
A new approach to structural sensing, fluorescence resonance energy transfer nanotomography, which interprets fluorescence decay measurement in terms of site density analysis of molecular distributions, has been applied to a glucose sensor based on competitive binding with malachite green labelled dextran to the sugar binding protein concanavalin A labelled with allophycocyanin. Opportunities for structural sensing in clinical medicine are highlighted.
Rolinski
O J
OJ
Department of Physics and Applied Physics, University of Strathclyde, Glasgow, UK.
Birch DJS
McCartney
L J
LJ
Pickup
J C
JC
D004396:Coloring Agents / Q000494:pharmacology; D003208:Concanavalin A / Q000032:analysis*; D004735:Energy Transfer; D005947:Glucose / Q000032:analysis*; D007477:Ions; D015233:Models, Statistical; D010798:Phycocyanin / Q000494:pharmacology*; D017434:Protein Structure, Tertiary; D013050:Spectrometry, Fluorescence / Q000379:methods*
D004396:Coloring Agents; D007477:Ions; C002497:allophycocyanin; D010798:Phycocyanin; D003208:Concanavalin A; D005947:Glucose