title: Mapping of quantitative trait locus related to submergence tolerance in rice with aid of chromosome walking.
abstract: The major QTL for submergence tolerance was locate in the 5.9 cM interval between flanking RFLP markers. To narrow down this region, a physical map was constructed using YAC and BAC clones. A 400-kb YAC was identified in this region and later its end fragments were used to screen a rice BAC library. Through chromosome walking, 24 positive BAC clones formed two contigs around linked-RFLP markers, R1164 and RZ698. Using one YAC end, six BAC ends and three RFLP markers, a fine-scale map was constructed of the 6.8-cM interval of S10709-RZ698 on rice chromosome 9. The submergence tolerance and related trait were located in a small, well-defined region around BAC-end marker 180D1R and RFLP marker R1164. The physical-to-map distance ratio in this region is as small as 172.5 kb/cM, showing that this region is a hot spot for recombination in the rice genome.
authors.affiliation: National Center for Genetic Engineering and Biotechnology, Kasetsart University, Nakorn Pathom, Thailand. wintai@dna.kps.ku.ac.th
authors.forename: W
authors.initials: W
authors.lastname: Kamolsukyunyong
authors.forename: V
authors.initials: V
authors.lastname: Ruanjaichon
authors.forename: M
authors.initials: M
authors.lastname: Siangliw
authors.forename: S
authors.initials: S
authors.lastname: Kawasaki
authors.forename: T
authors.initials: T
authors.lastname: Sasaki
authors.forename: A
authors.initials: A
authors.lastname: Vanavichit
authors.forename: S
authors.initials: S
authors.lastname: Tragoonrung
mesh_terms: D016386:Chromosome Walking; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics; D018244:Chromosomes, Artificial, Yeast / Q000235:genetics; D004190:Disasters; D017343:Genes, Plant; D005819:Genetic Markers; D012275:Oryza / Q000235:genetics* / Q000502:physiology; D010641:Phenotype; D020161:Physical Chromosome Mapping; D012150:Polymorphism, Restriction Fragment Length; D019655:Quantitative Trait, Heritable
chemical_list: D005819:Genetic Markers