title: Control of calcium homeostasis in Schistosoma mansoni.
abstract: Calcium signalling is fundamental for muscular contractility of Schistosoma mansoni. We have previously described the presence of transport ATPases (Na+,K+-ATPase and (Ca2+-Mg2+)-ATPase) and calcium channels (ryanodine receptors - RyR) involved in control of calcium homeostasis in this worm. Here we briefly review the main technics (ATPase activity, binding with specific radioligands, fluxes of 45Ca2+ and whole worm contractions) and results obtained in order to compare the distribution patterns of these proteins: thapsigargin-sensitive (Ca2+-Mg2+)-ATPase activity and RyR co-purified in P1 and P4 fractions mainly, which is compatible with a sarcoplasmic reticulum localization, while basal ATPase (along with Na+,K+-ATPase) and thapsigargin-resistant (Ca2+-Mg2+)-ATPase have a distinct distribution, indicative of their plasma membrane localization. Finally we attempt to integrate these contributions with data from other groups in order to propose the first synoptic model for control of calcium homeostasis in S. mansoni.
authors.affiliation: Departamento de Farmacologia Básica e Clínica, ICB, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, 21941-590, Brasil. fnoel@pharma.ufrj.br
authors.forename: F
authors.initials: F
authors.lastname: Noël
authors.forename: V M
authors.initials: VM
authors.lastname: Cunha
authors.forename: C L
authors.initials: CL
authors.lastname: Silva
authors.forename: D L
authors.initials: DL
authors.lastname: Mendonça-Silva
mesh_terms: D000818:Animals; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism; D002118:Calcium / Q000378:metabolism / Q000502:physiology*; D020013:Calcium Signaling; D006706:Homeostasis / Q000502:physiology*; D009130:Muscle, Smooth / Q000378:metabolism; D019837:Ryanodine Receptor Calcium Release Channel / Q000378:metabolism; D012550:Schistosoma mansoni / Q000378:metabolism*; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism
chemical_list: D019837:Ryanodine Receptor Calcium Release Channel; D017301:Ca(2+) Mg(2+)-ATPase; D000254:Sodium-Potassium-Exchanging ATPase; D002118:Calcium