Measurement of bisphenol A levels in human urine.
We report a new approach for assessing human exposure to bisphenol A (BPA) by measuring BPA in urine after enzymatic deglucuronidation. This method involves addition of (13)C(12)-labeled BPA, enzymatic deconjugation, solid-phase extraction, and derivatization with pentafluorobenzyl bromide. The product of the derivatization is separated by gas chromatography followed by mass spectrometric detection using negative chemical ionization and selected ion monitoring. Using this analysis method, urine samples fortified with both a constant level of labeled BPA and a range of unlabeled BPA levels (0.27-10.6 ng/ml) demonstrated constant percentage recovery. In addition, a range of urine sample volumes (0.25-10.0 ml) with constant amounts of added internal standard produced a linear response (r(2)=0.99). The method limit of detection was 0.12 ng/ml. This method was validated by duplicate analyses using gas chromatography coupled to a high-resolution mass spectrometer.
Brock
J W
JW
National Center for Environmental Health, Centers for Disease Control and Prevention (CDC), Atlanta, Georgia, USA. jwb6@cdc.gov
Yoshimura
Y
Y
Barr
J R
JR
Maggio
V L
VL
Graiser
S R
SR
Nakazawa
H
H
Needham
L L
LL
D001559:Benzhydryl Compounds; D002247:Carbon Isotopes; D002623:Chemistry Techniques, Analytical / Q000379:methods; D004781:Environmental Exposure; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D010636:Phenols / Q000652:urine*; D012016:Reference Values; D012680:Sensitivity and Specificity; D016482:Urinalysis; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D002247:Carbon Isotopes; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds