title: Vacuolar sorting determinants within a plant storage protein trimer act cumulatively.
abstract: The mechanism for vacuolar sorting of seed storage proteins is as yet poorly understood and no receptor has been identified to date. The homotrimeric glycoprotein phaseolin, which is the major storage protein of the common bean, requires a transient tetrapeptide at the C-terminus for its vacuolar sorting. A mutated construct without the tetrapeptide is secreted. We show here that coexpression of wild-type phaseolin and the mutated, secreted form in transgenic tobacco results in the formation of mixed trimers and partial vacuolar delivery of the mutated polypeptides and partial secretion of wild-type polypeptides. This indicates that the sorting signal has a cumulative effect within a phaseolin trimer. The result is discussed in the light of the hypothesized mechanisms for vacuolar sorting of seed storage proteins.
authors.affiliation: Istituto Biosintesi Vegetali, Consiglio Nazionale delle Ricerche, via Bassini 15, 20133 Milan, Italy.
authors.forename: H
authors.initials: H
authors.lastname: Holkeri
authors.forename: A
authors.initials: A
authors.lastname: Vitale
mesh_terms: D009154:Mutation / Q000235:genetics / Q000502:physiology; D009842:Oligopeptides / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000378:metabolism; D021382:Protein Sorting Signals / Q000502:physiology; D021381:Protein Transport / Q000502:physiology; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism; D014617:Vacuoles / Q000378:metabolism*
chemical_list: D009842:Oligopeptides; D010940:Plant Proteins; D021382:Protein Sorting Signals; C048826:phaseolin protein, Phaseolus vulgaris