Completion of the nucleotide sequence of the Enterococcus faecalis conjugative virulence plasmid pAD1 and identification of a second transfer origin.
pAD1 is a 59.3-kb plasmid in Enterococcus faecalis that has been the subject of intense investigation with regard to its pheromone-inducible conjugation behavior as well as its contribution to virulence. Approximately two-thirds of the pAD1 nucleotide sequence has been previously reported. Here we report on an analysis of the final approximately 22 kb, a significant portion of which is believed to encode structural genes associated with conjugation. The conjugation-related region was also found to contain a new (second) origin of conjugative transfer (oriT). A list of open reading frames covering the entire plasmid is presented.
Francia
M V
MV
Department of Biologic and Materials Sciences, School of Dentistry, University of Michigan, Ann Arbor, MI 48109, USA.
Haas
W
W
Wirth
R
R
Samberger
E
E
Muscholl-Silberhorn
A
A
Gilmore
M S
MS
Ike
Y
Y
Weaver
K E
KE
An
F Y
FY
Clewell
D B
DB
D003227:Conjugation, Genetic / Q000235:genetics*; D015246:Deoxyribonuclease EcoRI / Q000378:metabolism; D013293:Enterococcus faecalis / Q000235:genetics* / Q000472:pathogenicity*; D005798:Genes, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D010957:Plasmids / Q000235:genetics*; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
D015246:Deoxyribonuclease EcoRI