Study of different coupling agents in the conjugation of a V3-based synthetic MAP to carrier proteins.
The conjugation of synthetic peptides to carrier proteins is a widely used method for immunological studies. Different coupling agents have been described to form the conjugate with carrier proteins. In this paper, we demonstrate that the antibody response toward V3-based synthetic MAPs derived from HIV-1, JY1 isolate, conjugated to two different carrier proteins using either m-maleimidobenzoyl-N-hydroxysuccinimide ester (MBS) or beta-maleimidopropionic acid N-hydroxysuccinimide ester (MPS), or succinic anhydride (SA) show different behaviors. An excellent anti-JY1 response without a strong response to the coupling agent is observed in the case of succinic anhydride spacer. In contrast, MBS produces total abrogation of the antibody response with a high response toward the coupling agent.
Cruz
L J
LJ
División de Química-Física, Centro de Ingeniería Genética y Biotecnologia, Cubanacán, Habana, Cuba. ljcruz@cigb.edu.cu
Iglesias
E
E
Aguilar
J C
JC
Quintana
D
D
Garay
H E
HE
Duarte
C
C
Reyes
O
O
D000818:Animals; D000917:Antibody Formation; D002851:Chromatography, High Pressure Liquid; D003432:Cross-Linking Reagents / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007202:Indicators and Reagents / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D010452:Peptide Biosynthesis; D010455:Peptides / Q000737:chemistry; D011506:Proteins / Q000138:chemical synthesis; D013387:Succinic Anhydrides / Q000737:chemistry; D013388:Succinimides / Q000737:chemistry
D003432:Cross-Linking Reagents; D007202:Indicators and Reagents; D010455:Peptides; D011506:Proteins; D013387:Succinic Anhydrides; D013388:Succinimides; C072252:m-maleimidobenzoyl-N-hydroxysulfosuccinimide ester; C013097:3-maleimidobenzoyl N-hydroxysuccinimide; C031801:succinic anhydride