High intranuclear mobility and dynamic clustering of the splicing factor U1 snRNP observed by single particle tracking.
Uridine-rich small nuclear ribonucleoproteins (U snRNPs) are components of the splicing machinery that removes introns from precursor mRNA. Like other splicing factors, U snRNPs are diffusely distributed throughout the nucleus and, in addition, are concentrated in distinct nuclear substructures referred to as speckles. We have examined the intranuclear distribution and mobility of the splicing factor U1 snRNP on a single-molecule level. Isolated U1 snRNPs were fluorescently labeled and incubated with digitonin-permeabilized 3T3 cells in the presence of Xenopus egg extract. By confocal microscopy, U1 snRNPs were found to be imported into nuclei, yielding a speckled intranuclear distribution. Employing a laser video-microscope optimized for high sensitivity and high speed, single U1 snRNPs were visualized and tracked at a spatial precision of 35 nm and a time resolution of 30 ms. The single-particle data revealed that U1 snRNPs occurred in small clusters that colocalized with speckles. In the clusters, U1 snRNPs resided for a mean decay time of 84 ms before leaving the optical slice in the direction of the optical axis, which corresponded to a mean effective diffusion coefficient of 1 microm(2)/s. An analysis of the trajectories of single U1 snRNPs revealed that at least three kinetic classes of low, medium, and high mobility were present. Moreover, the mean square displacements of these fractions were virtually independent of time, suggesting arrays of binding sites. The results substantiate the view that nuclear speckles are not rigid structures but highly dynamic domains characterized by a rapid turnover of U1 snRNPs and other splicing factors.
Kues
T
T
Institut für Medizinische Physik und Biophysik, Westfälische Wilhelms-Universität, Robert-Koch-Strasse 31, D-48149 Münster, Germany.
Dickmanns
A
A
Lührmann
R
R
Peters
R
R
Kubitscheck
U
U
D016475:3T3 Cells; D000818:Animals; D002232:Carbocyanines; D002467:Cell Nucleus / Q000378:metabolism*; D005456:Fluorescent Dyes; D006834:Hydrazines; D051379:Mice; D012326:RNA Splicing; D017412:Ribonucleoprotein, U1 Small Nuclear / Q000378:metabolism*
C417561:Alexa 488 hydrazide; D002232:Carbocyanines; D005456:Fluorescent Dyes; D006834:Hydrazines; D017412:Ribonucleoprotein, U1 Small Nuclear; C085321:cyanine dye 5