title: The urokinase plasminogen activator receptor-associated protein/endo180 is coexpressed with its interaction partners urokinase plasminogen activator receptor and matrix metalloprotease-13 during osteogenesis.
abstract: The urokinase plasminogen activator receptor-associated protein/Endo180 (uPARAP/Endo180) is a newly discovered member of the macrophage mannose receptor family that was reported to interact with ligand-bound urokinase plasminogen activator receptor (uPAR), matrix metalloprotease-13 (MMP-13), and collagen V on the cell surface. We have determined the sites of expression of this novel receptor during murine postimplantation development. uPARAP/Endo180 was expressed in all tissues undergoing primary ossification, including the developing bones of the viscerocranium and calvarium that ossify intramembranously, and developing long bones undergoing endochondral ossification. uPARAP/Endo180 mRNA was expressed by both immature osteoblasts and by mature osteocalcin-producing osteoblasts-osteocytes, and was coexpressed with MMP-13. Interestingly, osteoblasts also expressed uPAR. Besides bone-forming tissues, uPARAP/Endo180 expression was detected only in a mesenchymal condensation of the midbrain and in the developing lungs. The data suggest a function of this novel protease receptor in bone development, possibly mediated through its interactions with uPAR, MMP-13, or collagen V.
authors.affiliation: Oral and Pharyngeal Cancer Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland, USA.
authors.forename: L H
authors.initials: LH
authors.lastname: Engelholm
authors.forename: B S
authors.initials: BS
authors.lastname: Nielsen
authors.forename: S
authors.initials: S
authors.lastname: Netzel-Arnett
authors.forename: H
authors.initials: H
authors.lastname: Solberg
authors.forename: X D
authors.initials: XD
authors.lastname: Chen
authors.forename: J M
authors.initials: JM
authors.lastname: Lopez Garcia
authors.forename: C
authors.initials: C
authors.lastname: Lopez-Otin
authors.forename: M F
authors.initials: MF
authors.lastname: Young
authors.forename: H
authors.initials: H
authors.lastname: Birkedal-Hansen
authors.forename: K
authors.initials: K
authors.lastname: Danø
authors.forename: L R
authors.initials: LR
authors.lastname: Lund
authors.forename: N
authors.initials: N
authors.lastname: Behrendt
authors.forename: T H
authors.initials: TH
authors.lastname: Bugge
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000196:embryology / Q000502:physiology*; D017364:Collagenases / Q000096:biosynthesis*; D005314:Embryonic and Fetal Development; D005260:Female; D007150:Immunohistochemistry; D053509:Matrix Metalloproteinase 13; D051379:Mice; D010012:Osteogenesis / Q000502:physiology; D011247:Pregnancy; D011956:Receptors, Cell Surface / Q000096:biosynthesis*; D011975:Receptors, Mitogen / Q000096:biosynthesis*; D055293:Receptors, Urokinase Plasminogen Activator
chemical_list: C408294:Endo180; C524899:Plaur protein, mouse; D011956:Receptors, Cell Surface; D011975:Receptors, Mitogen; D055293:Receptors, Urokinase Plasminogen Activator; D017364:Collagenases; D053509:Matrix Metalloproteinase 13; C507081:Mmp13 protein, mouse