title: Reticulum cell sarcoma of lymph node with mixed dendritic and fibroblastic features.
abstract: We report a case of clinically aggressive reticulum cell sarcoma with mixed follicular dendritic cell (FDC) and fibroblastic reticular cell (FRC) features. Histologically, the tumor was confined to lymph nodes occurring as a multifocal epithelioid and spindle cell proliferation with appreciable mitotic rate and numerous admixed non-neoplastic B-cells. Ultrastructural examination revealed elongated cells with prominent nucleoli, interdigitating cell processes and frequent desmosomes. These features are typical of FDC sarcoma. However, immunohistochemical stains showed no expression of antigens characteristic of FDCs, including CD21, CD23 and CD35. Cytogenetic characterization of this tumor, by conventional G-banding and multicolor spectral karyotyping, revealed multiple clonal chromosomal aberrations, including del(X)(p11.4) and add (21)(p11.2). Gene expression analysis by cDNA microarray of RNA obtained from short-term tumor cultures revealed high-level expression of a set of genes (including PDGF receptor-alpha and -beta, certain metalloproteinases, and CD105) that were also highly expressed in cultures of nodal FRC cultured from non-neoplastic lymph nodes. We propose that this tumor represents a nodal sarcoma with intermediate differentiation between FDCs and FRCs. This case adds to the diversity of tumors that may arise from lymph node stroma and supports a possible relationship between the FDC and FRC lineages.
authors.affiliation: Division of Pathology and Laboratory MedicineUniversity of Texas-M.D. Anderson Cancer Center, Houston, Texas 77030, USA. dajones@mdanderson.org
authors.forename: D
authors.initials: D
authors.lastname: Jones
authors.forename: M
authors.initials: M
authors.lastname: Amin
authors.forename: N G
authors.initials: NG
authors.lastname: Ordonez
authors.forename: A B
authors.initials: AB
authors.lastname: Glassman
authors.forename: K J
authors.initials: KJ
authors.lastname: Hayes
authors.forename: L J
authors.initials: LJ
authors.lastname: Medeiros
mesh_terms: D000328:Adult; D019013:CD40 Antigens / Q000032:analysis; D002869:Chromosome Aberrations; D020566:Dendritic Cells, Follicular / Q000473:pathology; D005347:Fibroblasts / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D007621:Karyotyping; D008198:Lymph Nodes / Q000473:pathology*; D008228:Lymphoma, Non-Hodgkin / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D008297:Male; D014407:Tumor Cells, Cultured / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure; D019010:Vascular Cell Adhesion Molecule-1 / Q000032:analysis
chemical_list: D019013:CD40 Antigens; D019010:Vascular Cell Adhesion Molecule-1