Active water selective channels in the stomach: investigation of aquaporins after ethanol and capsaicin treatment in rats.
Recent studies discovered the existence of aquaporins (AQP), suggesting their roles in the active, ATP dependent water secretion or absorption. Our recent development of the monoclonal antibody family against aquaporins (Type 1 and 4) allowed us a good opportunity to investigate the mechanism of the gastric mucosal edema in a rat model. THE AIM OF OUR STUDY was to evaluate the changes in the tissue level of aquaporins (AQP1 and AQP4) after ethanol and capsaicin treatment in rat stomach.


METHODS
the experiments were carried out on Sprague-Dawley rats weighing 150-200 g. The animals were fasted for 24 h, after the 1 ml of ethanol (50% v/v) or capsaicin (2 mg/ml) was given intragastrically. Rats were sacrificed after 5, 30, 60, 120 and 240 min, the tissue level of AQP1 and AQP4 was investigated immunoserologically by ELISA and dot-blot methods using our monoclonal antibodies. The location of these aquaporins in the gastric tissue was demonstrated by immunohistochemistry.


RESULTS
(1) in ethanol-treated stomach, both AQP1 and AQP4 increased after 5 min simultaneously with gastritis, then decreased dramatically depending on time. (2) In the capsaicin-treated group there were no changes in the tissue level of aquaporins in the first hour. After 60 min both AQP1 and AQP4 increased in the stomach without any macroscopically detectable changes, then decreased depending on time. (3) The immunohistochemical investigations using our monoclonal antibodies seem to support our present quantitative results.


CONCLUSIONS
chemically induced gastric mucosal lesions are started by an extended edema. In the induction of the edema and the subsequent gastric injury, aquaporins (both AQP1 and AQP4) play an important role in the maintenance of mucosal integrity.
Bódis
B
B
First Department of Medicine, Medical Faculty, University of Pecs, Hungary.
Nagy
G
G
Németh
P
P
Mózsik
G
G
D000818:Animals; D051398:Aquaporin 1; D051401:Aquaporin 4; D020346:Aquaporins / Q000378:metabolism*; D002211:Capsaicin / Q000494:pharmacology*; D004797:Enzyme-Linked Immunosorbent Assay; D000431:Ethanol / Q000494:pharmacology*; D005753:Gastric Mucosa / Q000378:metabolism*; D015151:Immunoblotting; D007150:Immunohistochemistry; D051381:Rats; D017207:Rats, Sprague-Dawley
C496022:Aqp1 protein, rat; C496032:Aqp4 protein, rat; D051401:Aquaporin 4; D020346:Aquaporins; D051398:Aquaporin 1; D000431:Ethanol; D002211:Capsaicin