title: Cold-induced increment in rat adrenal gland type II deiodinase is corticosterone dependent.
abstract: In this study we analyzed whether corticosterone synthesis is involved in the regulation of adrenal gland type II deiodinase (AG-D2) activity during acute cold exposure. Two well-known inhibitors of steroidogenesis, aminoglutethimide (AGT) and metyrapone (MTP), were administered to male Wistar rats maintained either at room temperature or acutely exposed to cold (1 h at 4 degrees C). AG-D2 activity was measured by the radioiodide release method, and corticosterone circulating levels were measured by competitive protein binding assay. Results show that resting corticosterone levels and AG-D2 activity were lower in both AGT- and MTP-treated rats. Furthermore, the phasic increase normally exhibited by AG-D2 activity in response to acute cold stress was blunted in AGT- and MTP-treated animals. Therefore, we conclude that corticosterone synthesis is necessary in preserving the physiologic response of AG-D2 activity to cold exposure.
authors.affiliation: Centro de Neurobiología, Universidad Nacional Autónoma de México, Querétaro.
authors.forename: B
authors.initials: B
authors.lastname: Anguiano
authors.forename: C
authors.initials: C
authors.lastname: Valverde
mesh_terms: D000311:Adrenal Glands / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D000616:Aminoglutethimide / Q000494:pharmacology; D000818:Animals; D001667:Binding, Competitive; D002786:Cholesterol Side-Chain Cleavage Enzyme / Q000037:antagonists & inhibitors; D003080:Cold Temperature; D003345:Corticosterone / Q000097:blood / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D007453:Iodide Peroxidase / Q000378:metabolism*; D007457:Iodine Radioisotopes; D008297:Male; D008797:Metyrapone / Q000494:pharmacology; D009929:Organ Size / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D013252:Steroid 11-beta-Hydroxylase / Q000037:antagonists & inhibitors
chemical_list: D004791:Enzyme Inhibitors; D007457:Iodine Radioisotopes; D000616:Aminoglutethimide; D007453:Iodide Peroxidase; D013252:Steroid 11-beta-Hydroxylase; D002786:Cholesterol Side-Chain Cleavage Enzyme; D003345:Corticosterone; D008797:Metyrapone