title: mRNA expression of cytokines and chemokines in the normal gastric surface mucous epithelial cell line GSM06 during bacterial infection with Helicobacter felis.
abstract: OBJECTIVE
  A group of the proinflammatory and chemotactic cytokines (chemokines) has been considered as an important factor in the pathomechanism of different bacterial diseases, among them the common Helicobacter pylori infection. Experimental results obtained with gastric biopsy samples of H. pylori positive patients, and with H. pylori infected tumor originated gastric cell lines indicated that these cytokines have essential roles in the development and maintenance of the immune response and inflammation of the gastric mucosa during H. pylori infection. Although the mRNA expression was shown in these biopsy samples and cell lines, it is not yet proved that the normal gastric mucosal epithelial cells themselves express these cytokines. The establishment of a gastric surface mucous cell line with non-tumor origin (GSM06), and the usage of Helicobacter felis as a model of the classic H. pylori infection gave us the possibility to check this question.
  
  
  METHODS
  in this study GSM06 cells were infected with different numbers (10(5), 10(6), 10(7), 10(8), 10(9) bacterium/ml medium) of H. felis for two different time periods (2, 4 h). Cells treated with medium only were used as control. Then the mRNA expression of the following cytokines was measured by RT-PCR method in the GSM06 cells: proinflammatory cytokine IL1-beta, and chemokine RANTES, eotaxin, MCP-1, MIP1-alpha and MIP1-beta.
  
  
  RESULTS
  we found that neither mRNA of the investigated cytokines was expressed constitutively, however the GSM06 cells expressed the mRNA of each cytokine during H. felis infection.
  
  
  CONCLUSIONS
  our results prove that normal gastric surface mucous epithelial cells express immunologically active peptides during H. felis infection. We may suppose that the epithelial cells of the gastric mucosa contribute to the immune response and inflammation by expressing proinflammatory (IL1-beta) and chemotactic (RANTES, eotaxin, MCP-1, MIP1-alpha and beta) cytokines during H. pylori infection in human.
authors.affiliation: Department of Gastroenterology and Hepatology, Faculty of Medicine, Kyoto University, Shogoin-Kawaharamachi, Sakyo-ku, 606 Kyoto, Japan. deband@clinics.pote.hu
authors.forename: A
authors.initials: A
authors.lastname: Debreceni
authors.forename: K
authors.initials: K
authors.lastname: Okazaki
authors.forename: Y
authors.initials: Y
authors.lastname: Matsushima
authors.forename: M
authors.initials: M
authors.lastname: Ohana
authors.forename: H
authors.initials: H
authors.lastname: Nakase
authors.forename: K
authors.initials: K
authors.lastname: Uchida
authors.forename: S
authors.initials: S
authors.lastname: Uose
authors.forename: T
authors.initials: T
authors.lastname: Chiba
mesh_terms: D000818:Animals; D002460:Cell Line; D018925:Chemokines / Q000235:genetics*; D016207:Cytokines / Q000235:genetics*; D005753:Gastric Mucosa / Q000166:cytology / Q000378:metabolism*; D016481:Helicobacter Infections / Q000378:metabolism*; D051379:Mice; D012333:RNA, Messenger / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors
chemical_list: D018925:Chemokines; D016207:Cytokines; D012333:RNA, Messenger