title: Polycystin-1 interacts with intermediate filaments.
abstract: Polycystin-1, the protein defective in a majority of patients with autosomal dominant polycystic kidney disease, is a ubiquitously expressed multi-span transmembrane protein of unknown function. Subcellular localization studies found this protein to be a component of various cell junctional complexes and to be associated with the cytoskeleton, but the specificity and nature of such associations are not known. To identify proteins that interact with the polycystin-1 C-tail (P1CT), this segment was used as bait in a yeast two-hybrid screening of a kidney epithelial cell library. The intermediate filament (IF) protein vimentin was identified as a strong polycystin-1-interacting partner. Cytokeratins K8 and K18 and desmin were also found to interact with P1CT. These interactions were mediated by coiled-coil motifs in polycystin-1 and IF proteins. Vimentin, cytokeratins K8 and K18, and desmin also bound directly to P1CT in GST pull-down and in in vitro filament assembly assays. Two observations confirmed these interactions in vivo: (i) a cell membrane-anchored form of recombinant P1CT decorated the IF network and was found to associate with the cytoskeleton in detergent-solubilized cells and (ii) endogenous polycystin-1 distributed with IF at desmosomal junctions. Polycystin-1 may utilize this association for structural, storage, or signaling functions.
authors.affiliation: Renal Unit, Massachusetts General Hospital and Harvard Medical School, Charlestown, Massachusetts 02129, USA.
authors.forename: G M
authors.initials: GM
authors.lastname: Xu
authors.forename: T
authors.initials: T
authors.lastname: Sikaneta
authors.forename: B M
authors.initials: BM
authors.lastname: Sullivan
authors.forename: Q
authors.initials: Q
authors.lastname: Zhang
authors.forename: M
authors.initials: M
authors.lastname: Andreucci
authors.forename: T
authors.initials: T
authors.lastname: Stehle
authors.forename: I
authors.initials: I
authors.lastname: Drummond
authors.forename: M A
authors.initials: MA
authors.lastname: Arnaout
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D003599:Cytoskeleton / Q000378:metabolism; D018076:DNA, Complementary; D004285:Dogs; D005455:Fluorescent Antibody Technique; D005982:Glutathione Transferase / Q000378:metabolism; D006801:Humans; D007381:Intermediate Filament Proteins / Q000378:metabolism*; D007633:Keratins / Q000378:metabolism; D007700:Kinetics; D018374:LLC-PK1 Cells; D008969:Molecular Sequence Data; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D013552:Swine; D050396:TRPP Cation Channels; D020798:Two-Hybrid System Techniques
chemical_list: D018076:DNA, Complementary; D005982:Glutathione Transferase; D007381:Intermediate Filament Proteins; D007633:Keratins; D011506:Proteins; D050396:TRPP Cation Channels