title: Positively charged termini of the L2 minor capsid protein are necessary for papillomavirus infection.
abstract: Coexpression of bovine papillomavirus L1 with L2 mutants lacking either eight N-terminal or nine C-terminal amino acids that encode positively charged domains resulted in wild-type levels of viral genome encapsidation. Despite wild-type binding to the cell surface, the resulting virions were noninfectious. An L2 mutant encoding a scrambled version of the nine C-terminal residues restored infectivity, in contrast to an L2 mutant encoding a scrambled version of the N-terminal residues.
authors.affiliation: Department of Pathology, The Johns Hopkins University, Baltimore, Maryland 21205-2196, USA. roden@jhmi.edu
authors.forename: R B
authors.initials: RB
authors.lastname: Roden
authors.forename: P M
authors.initials: PM
authors.lastname: Day
authors.forename: B K
authors.initials: BK
authors.lastname: Bronzo
authors.forename: W H
authors.initials: WH
authors.lastname: Yutzy
authors.forename: Y
authors.initials: Y
authors.lastname: Yang
authors.forename: D R
authors.initials: DR
authors.lastname: Lowy
authors.forename: J T
authors.initials: JT
authors.lastname: Schiller
mesh_terms: D000818:Animals; D052688:Bovine papillomavirus 1 / Q000502:physiology*; D002213:Capsid / Q000737:chemistry / Q000502:physiology*; D036022:Capsid Proteins; D002460:Cell Line; D006224:Cricetinae; D009363:Neoplasm Proteins / Q000032:analysis; D009687:Nuclear Proteins; D014157:Transcription Factors / Q000032:analysis; D025521:Tumor Suppressor Proteins; D014771:Virion / Q000502:physiology; D019065:Virus Assembly
chemical_list: D036022:Capsid Proteins; C085217:L1 protein, Bovine papillomavirus; C085218:L2 protein, Bovine papillomavirus; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D014157:Transcription Factors; D025521:Tumor Suppressor Proteins