Gut expression and regulation of FAT/CD36: possible role in fatty acid transport in rat enterocytes.
Fatty acid translocase (FAT)/CD36 is one of several putative plasma membrane long-chain fatty acid (LCFA) transport proteins; however, its role in intestinal absorption of LCFA is unknown. We hypothesized that FAT/CD36 would be differentially expressed along the longitudinal axis of the gut and during intestinal development, suggesting specificity of function. We found that intestinal mucosal FAT/CD36 mRNA levels varied by anatomic location along the longitudinal gut axis: stomach 45 +/- 7, duodenum 173 +/- 29, jejunum 238 +/- 17, ileum 117 +/- 14, and colon 9 +/- 1% (means +/- SE with 18S mRNA as control). FAT/CD36 protein levels were also higher in proximal compared with distal intestinal mucosa. Mucosal FAT/CD36 mRNA was also regulated during intestinal maturation, with a fourfold increase from neonatal to adult animals. In addition, FAT/CD36 mRNA levels and enterocyte LCFA uptake were rapidly downregulated by intraduodenal oleate infusion. These findings suggest that FAT/CD36 plays a role in the uptake of LCFA by small intestinal enterocytes. This may have important implications in understanding fatty acid absorption in human physiological and pathophysiological conditions.
Chen
M
M
Department of Surgery, University of Alabama at Birmingham, 35233, USA.
Yang
Y
Y
Braunstein
E
E
Georgeson
K E
KE
Harmon
C M
CM
D000818:Animals; D000911:Antibodies, Monoclonal; D001692:Biological Transport / Q000187:drug effects; D015153:Blotting, Western; D018955:CD36 Antigens / Q000235:genetics* / Q000502:physiology; D003106:Colon / Q000737:chemistry / Q000378:metabolism; D004064:Digestive System / Q000254:growth & development / Q000378:metabolism*; D004386:Duodenum / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D020895:Enterocytes / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism*; D005753:Gastric Mucosa / Q000737:chemistry / Q000378:metabolism; D005786:Gene Expression Regulation; D007082:Ileum / Q000737:chemistry / Q000378:metabolism; D007408:Intestinal Absorption; D007413:Intestinal Mucosa / Q000737:chemistry / Q000378:metabolism; D007583:Jejunum / Q000737:chemistry / Q000378:metabolism; D007700:Kinetics; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000502:physiology; D019301:Oleic Acid / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology; D027361:Organic Anion Transporters / Q000235:genetics* / Q000502:physiology; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D014316:Tritium
D000911:Antibodies, Monoclonal; D018955:CD36 Antigens; D005227:Fatty Acids; D008562:Membrane Glycoproteins; D027361:Organic Anion Transporters; D012333:RNA, Messenger; D014316:Tritium; D019301:Oleic Acid