Imaging of hydroperoxide and hydrogen peroxide-scavenging substances by photon emission.
An imaging system for the hydroperoxide and hydrogen donor was developed by photon emission from the reactive oxygen species-hydrogen donor-KHCO(3)-MeCHO system. Photon intensity (luminance, cd/m(2)) showed a linear correlation with the concentration of some hydroperoxides [hydrogen peroxide (H(2)O(2)), tert-butyl hydroperoxide and methyl ethyl ketone peroxide] and that of hydrogen donors (catechins and anthocyanins). The linear relationship between photon intensity and concentration was observed in polyphenol-rich samples, such as teas, berries and currants. Photon intensity from the H(2)O(2)/polyphenol-rich sample-KHCO(3)-MeCHO system corresponded with H(2)O(2)-scavenging activity, rather than with polyphenol contents. It is possible to classify hydrogen donor species according to absorption features using spectral analysis (gallic acid, E(max) 645 nm, catechins, E(max) 610 nm; anthocyanins, E(max) 690-700 nm). This method is a simple and sensitive detection system for hydroperoxide- and H(2)O(2)-scavenging substances.
Yoshiki
Y
Y
Department of Environmental Bioremediation, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori Amamiyamachi, Aoba-ku, Sendai, Miyagi 981-8555, Japan. yoshiki@bios.tohoku.ac.jp
Iida
T
T
Akiyama
Y
Y
Okubo
K
K
Matsumoto
H
H
Sato
M
M
D000079:Acetaldehyde / Q000737:chemistry*; D000872:Anthocyanins; D002392:Catechin / Q000737:chemistry; D004791:Enzyme Inhibitors / Q000737:chemistry; D005504:Food Analysis / Q000379:methods; D005638:Fruit / Q000737:chemistry; D005707:Gallic Acid / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000032:analysis / Q000737:chemistry*; D007529:Isoflavones / Q000737:chemistry; D008163:Luminescent Measurements; D017785:Photons; D017382:Reactive Oxygen Species / Q000032:analysis / Q000737:chemistry*; D013057:Spectrum Analysis; D013662:Tea / Q000737:chemistry
D000872:Anthocyanins; D004791:Enzyme Inhibitors; D007529:Isoflavones; D017382:Reactive Oxygen Species; D013662:Tea; D005707:Gallic Acid; D002392:Catechin; D006861:Hydrogen Peroxide; D000079:Acetaldehyde