title: Tumour-associated antigen (TAA)-specific cytotoxic T cell (CTL) response in vitro and in a mouse model, induced by TAA-plasmids delivered by influenza virosomes.
abstract: We investigated influenza virosomes as a TAA-gene delivery system for use in TAA-directed anti-cancer vaccine therapy. An engineered plasmid (GC90) expressing the parathyroid hormone-related peptide (PTH-rP), a protein secreted by prostate and lung carcinoma cells, was included in influenza virosomes (GC90V). The ability of GC90V to elicit a PTH-rP-specific cytotoxic T cell (CTL) response was demonstrated in BALB/c mice immunised with intranasal (i.n.) GC90V+/-adjuvant subcutaneous (s.c.) interleukin-2 (IL-2). A PTH-rP-specific CTL response with antitumour activity was also demonstrated in human peripheral blood mononuclear cells (PBMC) stimulated in vitro with GC90V infected autologous dendritic cells (DC). These results provide a rationale for investigating GC90V in clinical trials of anticancer vaccine therapy.
authors.affiliation: Medical Oncology Division, Medicine School, Siena University, 53100, Siena, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Correale
authors.forename: M G
authors.initials: MG
authors.lastname: Cusi
authors.forename: M
authors.initials: M
authors.lastname: Sabatino
authors.forename: L
authors.initials: L
authors.lastname: Micheli
authors.forename: D
authors.initials: D
authors.lastname: Pozzessere
authors.forename: C
authors.initials: C
authors.lastname: Nencini
authors.forename: P E
authors.initials: PE
authors.lastname: Valensin
authors.forename: R
authors.initials: R
authors.lastname: Petrioli
authors.forename: G
authors.initials: G
authors.lastname: Giorgi
authors.forename: R
authors.initials: R
authors.lastname: Zurbriggen
authors.forename: R
authors.initials: R
authors.lastname: Gluck
authors.forename: G
authors.initials: G
authors.lastname: Francini
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D000951:Antigens, Neoplasm / Q000235:genetics / Q000276:immunology*; D019496:Cancer Vaccines / Q000008:administration & dosage* / Q000276:immunology; D018929:Cell Culture Techniques; D003602:Cytotoxicity, Immunologic / Q000276:immunology*; D003713:Dendritic Cells / Q000276:immunology; D005260:Female; D018014:Gene Transfer Techniques; D006801:Humans; D009980:Influenza A virus / Q000235:genetics; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009363:Neoplasm Proteins / Q000235:genetics / Q000276:immunology; D044162:Parathyroid Hormone-Related Protein; D010957:Plasmids; D011506:Proteins / Q000235:genetics / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014162:Transfection / Q000379:methods; D014407:Tumor Cells, Cultured; D022701:Virosomes
chemical_list: D000951:Antigens, Neoplasm; D019496:Cancer Vaccines; D009363:Neoplasm Proteins; C470121:PTHLH protein, human; D044162:Parathyroid Hormone-Related Protein; D011506:Proteins; D022701:Virosomes