title: Non-steroidal anti-inflammatory agent ibuprofen-induced apoptosis, cell necrosis and cell cycle alterations in human leukemic cells in vitro.
abstract: The cytotoxic activity of the non-steroidal anti-inflammatory agent ibuprofen to human promyelocytic leukemia cell line HL-60, its multidrug-resistant subline HL-60/VCR (MDR-1 gene coded P-glycoprotein), as well as myeloma U266 and B-lymphoblastoid ARH-77 cell lines was demonstrated with the aid of flow cytometric analysis. Ibuprofen inhibited proliferation and induced apoptosis (detected as sub-G0 nuclei, fluorescein diacetate staining, Annexin-V binding cells and agarose electrophoretic detection of nucleosomal DNA fragmentation) in promyelocytic cells and, to a lesser extent, in U266 and ARH-77 cells.
authors.affiliation: Cancer Research Institute, Slovak Academy of Sciences, Bratislava, Slovak Republic. exonjana@savba.sk
authors.forename: J
authors.initials: J
authors.lastname: Jakubíková
authors.forename: T
authors.initials: T
authors.lastname: Duraj
authors.forename: X
authors.initials: X
authors.lastname: Takácsová
authors.forename: L
authors.initials: L
authors.lastname: Hunáková
authors.forename: B
authors.initials: B
authors.lastname: Chorváth
authors.forename: J
authors.initials: J
authors.lastname: Sedlák
mesh_terms: D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology* / Q000627:therapeutic use*; D017209:Apoptosis / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects*; D004587:Electrophoresis, Agar Gel; D005434:Flow Cytometry; D019450:Genes, MDR / Q000187:drug effects; D018922:HL-60 Cells / Q000187:drug effects; D006801:Humans; D007052:Ibuprofen / Q000494:pharmacology* / Q000627:therapeutic use*; D020128:Inhibitory Concentration 50; D007951:Leukemia, Myeloid / Q000188:drug therapy*; D014407:Tumor Cells, Cultured / Q000187:drug effects
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D007052:Ibuprofen