title: Epidemiologic usefulness of spoligotyping for secondary typing of Mycobacterium tuberculosis isolates with low copy numbers of IS6110.
abstract: Restriction fragment length polymorphism (RFLP) analysis of IS6110 is commonly used to DNA fingerprint Mycobacterium tuberculosis. However, low-copy (< or =5) IS6110 M. tuberculosis strains are poorly differentiated, requiring secondary typing. When spoligotyping was used as the secondary method, only 13% of Maryland culture-positive tuberculosis (TB) patients with low-copy IS6110-spoligotyped clustered strains had epidemiologic linkages to another patient, compared to 48% of those with high-copy strains clustered by IS6110 alone (P < 0.01). Spoligotyping did not improve a population-based molecular epidemiologic study of recent TB transmission.
authors.affiliation: Division of TB Control, Refugee and Migrant Health, University of Maryland-Baltimore, Baltimore, Maryland 21201, USAcroninw@dhmh.state.md.us.
authors.forename: W A
authors.initials: WA
authors.lastname: Cronin
authors.forename: J E
authors.initials: JE
authors.lastname: Golub
authors.forename: L S
authors.initials: LS
authors.lastname: Magder
authors.forename: N G
authors.initials: NG
authors.lastname: Baruch
authors.forename: M J
authors.initials: MJ
authors.lastname: Lathan
authors.forename: L N
authors.initials: LN
authors.lastname: Mukasa
authors.forename: N
authors.initials: N
authors.lastname: Hooper
authors.forename: J H
authors.initials: JH
authors.lastname: Razeq
authors.forename: D
authors.initials: D
authors.lastname: Mulcahy
authors.forename: W H
authors.initials: WH
authors.lastname: Benjamin
authors.forename: W R
authors.initials: WR
authors.lastname: Bishai
mesh_terms: D000368:Aged; D015373:Bacterial Typing Techniques; D016172:DNA Fingerprinting; D004251:DNA Transposable Elements / Q000235:genetics*; D018628:Gene Dosage; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000145:classification* / Q000235:genetics; D009841:Oligonucleotides / Q000032:analysis; D012150:Polymorphism, Restriction Fragment Length; D014397:Tuberculosis, Pulmonary / Q000453:epidemiology* / Q000382:microbiology
chemical_list: D004251:DNA Transposable Elements; D009841:Oligonucleotides