Expression of the FAE1 gene and FAE1 promoter activity in developing seeds of Arabidopsis thaliana.
Plant fatty acid elongase which catalyzes very-long-chain fatty acid (VLCFA) biosynthesis is a membrane-bound multienzyme complex. It is composed of four enzymes, a 3-ketoacyl-CoA synthase (condensing enzyme), a 3-ketoacyl-CoA reductase, a 3-hydroxyacyl-CoA dehydrase, and an enoyl-CoA reductase required for completion of each step of 2-carbon elongation of fatty acids. To improve our understanding of the overall regulation of the fatty acid elongase, we investigated the spatial and temporal expression of its key component, the FAE1-condensing enzyme, and examined the activity of the promoter of the FAE1 gene in Arabidopsis. In situ hybridization results revealed that FAE1 transcripts were found exclusively in the embryo. RNA blot analysis and histochemical analysis of GUS activity in pFAE1::GUS transgenic Arabidopsis lines demonstrated that the FAE1 gene was already transcribed in the early torpedo stage embryos 4-5 days after flowering, with transcription reaching its peak 9-11 days after flowering. VLCFA deposition closely paralleled FAE1 transcript accumulation. FAE1 promoter was highly active and embryo-specific. Because its timing coincides with the period of major storage lipid accumulation, and because its in vivo activity in Arabidopsis is superior to the napin promoter, FAE1 promoter may be ideal for genetic engineering of seed oil composition.
Rossak
M
M
Department of Botany, University of British Columbia, Vancouver, Canada.
Smith
M
M
Kunst
L
L
D000123:Acetyltransferases / Q000235:genetics*; D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics*; D001483:Base Sequence; D017931:DNA Primers; D000081031:Fatty Acid Elongases; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D017403:In Situ Hybridization; D011401:Promoter Regions, Genetic; D012639:Seeds / Q000378:metabolism*
D017931:DNA Primers; D000123:Acetyltransferases; D000081031:Fatty Acid Elongases