Isolation of methyl parathion-degrading strain M6 and cloning of the methyl parathion hydrolase gene.
A degradative bacterium, M6, was isolated and presumptively identified as Plesiomonas sp. strain M6 was able to hydrolyze methyl parathion to p-nitrophenol. A novel organophosphate hydrolase gene designated mpd was selected from its genomic library prepared by shotgun cloning. The nucleotide sequence of the mpd gene was determined. The gene could be effectively expressed in Escherichia coli.
Zhongli
C
C
Department of Microbiology, Nanjing Agricultural University, Nanjing, Jiangsu Province 210095, People's Republic of China.
Shunpeng
L
L
Guoping
F
F
D001673:Biodegradation, Environmental; D003001:Cloning, Molecular; D004755:Enterobacteriaceae / Q000145:classification / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification*; D008743:Methyl Parathion / Q000378:metabolism*; D008969:Molecular Sequence Data; D010744:Phosphoric Monoester Hydrolases / Q000235:genetics* / Q000378:metabolism*; D017422:Sequence Analysis, DNA
D008743:Methyl Parathion; C045290:phosphorylphosphatase; D010744:Phosphoric Monoester Hydrolases