Longitudinal variability of human immunodeficiency virus type 1 RNA viral load measurements by nucleic acid sequence-based amplification and NucliSens assays in a large multicenter study.
Human immunodeficiency virus type 1 (HIV-1) RNA measurements were evaluated within an externally controlled multilaboratory program. Three external standards (1.5 x 10(3) to 1.5 x 10(6) copies/ml) were included in 814 assay runs by four laboratories. Results indicate that HIV-1 RNA levels can be measured with a precision equal to that of the pre-highly active antiretroviral therapy era (standard deviations, +/-0.16 to 0.25 log10 units).
Nowicki
M J
MJ
University of Southern California, Pediatrics, Los Angeles, California 90033, USA. marek@usc.edu
Benning
L
L
Bremer
J W
JW
Meyer
W A
WA
Hanson
C
C
Brambilla
D
D
Silver
S
S
Kovacs
A
A
D015658:HIV Infections / Q000175:diagnosis* / Q000821:virology; D015497:HIV-1 / Q000502:physiology*; D007753:Laboratories; D021141:Nucleic Acid Amplification Techniques / Q000379:methods / Q000592:standards*; D011786:Quality Control; D012367:RNA, Viral / Q000097:blood*; D012016:Reference Values; D021161:Self-Sustained Sequence Replication / Q000379:methods / Q000592:standards*; D019562:Viral Load; D014773:Virology
D012367:RNA, Viral