title: Comparison of different electrophoretic separations of hen egg white proteins.
abstract: The hen egg white protein composition has not yet been fully defined. To improve the knowledge of this biological fluid, the most usual and recently developed electrophoretic methods have been used: SDS-PAGE, native-PAGE, isoelectric focusing (IEF), and 2-dimensional electrophoresis (2DE). Seven of the major known proteins were thus identified in at least one electrophoretic system. Isoforms of ovotransferrin, ovalbumin, and ovomucoid were visualized when pI was used for the separation. Two-dimensional electrophoresis allowed separation of a very large number of spots. In each of the four systems, some components were revealed but not identified, and unknown spots were particularly numerous with 2DE. With this technique, many spots corresponding to small acidic proteins were highlighted, among which was the Ch21 protein, whose presence in hen egg white was thus confirmed. This study thus constitutes, to our knowledge, the first proteomic investigation of hen egg white.
authors.affiliation: Ecole Nationale Supérieure Agronomique de Rennes and Research Laboratory for Dairy Technology (LRTL), Institut National de la Recherche Agronomique, 65 rue de Saint Brieuc, 35042 Rennes Cedex France.
authors.forename: C
authors.initials: C
authors.lastname: Desert
authors.forename: C
authors.initials: C
authors.lastname: Guérin-Dubiard
authors.forename: F
authors.initials: F
authors.lastname: Nau
authors.forename: G
authors.initials: G
authors.lastname: Jan
authors.forename: F
authors.initials: F
authors.lastname: Val
authors.forename: J
authors.initials: J
authors.lastname: Mallard
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002645:Chickens; D003207:Conalbumin / Q000302:isolation & purification; D004527:Egg Proteins / Q000302:isolation & purification*; D004586:Electrophoresis / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D007525:Isoelectric Focusing / Q000379:methods; D007526:Isoelectric Point; D010047:Ovalbumin / Q000302:isolation & purification; D010059:Ovomucin / Q000302:isolation & purification
chemical_list: D004527:Egg Proteins; D003207:Conalbumin; D010059:Ovomucin; D010047:Ovalbumin