Molecular cloning of a gene encoding matrix metalloproteinase-like protein from Gnathostoma spinigerum.
The advanced third-stage larvae (aL3) of Gnathostoma spinigerum contain a 24 kDa glycoprotein with diagnostic potential. Immunoscreening with the monoclonal antibody to the 24-kDa protein (mAb GN6/ 24) has identified a cDNA clone with an insert of 932 base pairs (bp). The insert contains a full-length gene of 732 bp encoding a protein that is 33-39% similar to matrix metalloproteinases (MMPs) of Caenorhabditis elegans and several lower and higher vertebrates. The MMP-like protein of G. spinigerum possesses the catalytic domain, but lacks the propeptide and hemopexin-like domains found in other MMPs. A signal peptide of 23 amino acids at its amino terminus indicates that it is a secretory protein, which is confirmed by Western blot analysis showing the presence of the 24 kDa protein in the excretory-secretory products of aL3.
Uparanukraw
P
P
Department of Parasitology, Faculty of Medicine, Chiang Mai University, Thailand. puparanu@mail.med.cmu.ac.th
Morakote
N
N
Harnnoi
T
T
Dantrakool
A
A
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins; D006039:Gnathostoma / Q000201:enzymology*; D015801:Helminth Proteins / Q000235:genetics / Q000378:metabolism; D020782:Matrix Metalloproteinases / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D018076:DNA, Complementary; D006023:Glycoproteins; D015801:Helminth Proteins; D020782:Matrix Metalloproteinases; C450661:matrix metalloproteinase-like protein, Gnathostoma spinigerum