Classification of metal-resistant bacteria from industrial biotopes as Ralstonia campinensis sp. nov., Ralstonia metallidurans sp. nov. and Ralstonia basilensis Steinle et al. 1998 emend.
Thirty-one heavy-metal-resistant bacteria isolated from industrial biotopes were subjected to polyphasic characterization, including 16S rDNA sequence analysis, DNA-DNA hybridizations, biochemical tests, whole-cell protein and fatty-acid analyses. All strains were shown to belong to the Ralstonia branch of the beta-Proteobacteria. Whole-cell protein profiles and DNA-DNA hybridizations revealed two clearly distinct groups, showing low similarity to known Ralstonia species. These two groups, of 8 and 17 isolates, were assigned to two new species, for which the names Ralstonia campinensis sp. nov. and Ralstonia metallidurans sp. nov. are proposed. The type strains are WS2T (= LMG 19282T = CCUG 44526T) and CH34T (= LMG 1195T = DSM 2839T), respectively. Six isolates were allocated to Ralstonia basilensis, which presently contains only the type strain; an emendation of the latter species description is therefore proposed.
Goris
J
J
De Vos
P
P
Coenye
T
T
Hoste
B
B
Janssens
D
D
Brim
H
H
Diels
L
L
Mergeay
M
M
Kersters
K
K
Vandamme
P
P
D001426:Bacterial Proteins / Q000737:chemistry; D001482:Base Composition; D020562:Betaproteobacteria / Q000145:classification* / Q000187:drug effects* / Q000378:metabolism; D004275:DNA, Ribosomal / Q000032:analysis; D024881:Drug Resistance, Bacterial; D004591:Electrophoresis, Polyacrylamide Gel; D005227:Fatty Acids / Q000032:analysis; D007218:Industrial Microbiology; D019216:Metals, Heavy / Q000494:pharmacology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
D001426:Bacterial Proteins; D004275:DNA, Ribosomal; D005227:Fatty Acids; D019216:Metals, Heavy; D012336:RNA, Ribosomal, 16S