Physiology of morphologically identified cells of the bullfrog fungiform papilla.
Voltage-gated ionic current and the response to quinine were studied on the four types of morphologically identified taste cells of the bullfrog fungiform papilla by whole-cell patch clamp recording with a Lucifer yellow-filled pipette. Dye-coupled type Ia cells (mucous cells) did not show voltage-activated currents. Type Ib cells (wing cells) characterized by the fin-like processes, type II cells (rod cells) having a thick straight dendrite running to the surface and type III cells with a thin dendrite had voltage-gated sodium (INa) and potassium currents (IK) and generated action potentials. The amplitude of INa was significantly larger in type Ib and II cells than in type III cells. Type Ib and II cells responded to quinine but Type III cells did not.
Takeuchi
H
H
Department of Physiology, Keio University School of Medicine, Tokyo 160-8582, Japan.
Tsunenari
T
T
Kurahashi
T
T
Kaneko
A
A
D000200:Action Potentials / Q000187:drug effects / Q000502:physiology*; D000818:Animals; D005456:Fluorescent Dyes / Q000493:pharmacokinetics; D007473:Ion Channels / Q000187:drug effects / Q000502:physiology*; D007546:Isoquinolines / Q000493:pharmacokinetics; D009924:Organ Culture Techniques; D018408:Patch-Clamp Techniques; D011803:Quinine / Q000494:pharmacology; D011892:Rana catesbeiana / Q000033:anatomy & histology / Q000502:physiology*; D013649:Taste / Q000187:drug effects / Q000502:physiology*; D013650:Taste Buds / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*
D005456:Fluorescent Dyes; D007473:Ion Channels; D007546:Isoquinolines; C017475:lucifer yellow; D011803:Quinine