title: Distinct mRNA microarray profiles of tolerogenic dendritic cells.
abstract: Dendritic cells are crucial to the activation as well as suppression of the immune response. Previous reports have illustrated that APC interacting with antigen-specific T suppressor cells become tolerogenic, inducing T helper anergy. To characterize the molecular changes occurring in tolerogenic APC, the mRNA profile of KG-1 dendritic cells exposed to allospecific T helper and T suppressor cells were analyzed. This study now provides evidence that immature dendritic cells stimulated by T suppressor cells differentiate into mature dendritic cells with a distinct phenotype. The identification of Ts induced pathways of dendritic cell differentiation is critical to the development of new therapeutic strategies.
authors.affiliation: Department of Pathology, Columbia University, New York, NY 10032, USA. ns20@columbia.edu
authors.forename: N
authors.initials: N
authors.lastname: Suciu-Foca Cortesini
authors.forename: F
authors.initials: F
authors.lastname: Piazza
authors.forename: E
authors.initials: E
authors.lastname: Ho
authors.forename: R
authors.initials: R
authors.lastname: Ciubotariu
authors.forename: J
authors.initials: J
authors.lastname: LeMaoult
authors.forename: R
authors.initials: R
authors.lastname: Dalla-Favera
authors.forename: R
authors.initials: R
authors.lastname: Cortesini
mesh_terms: D000943:Antigens, Differentiation; D017209:Apoptosis / Q000235:genetics / Q000276:immunology; D002450:Cell Communication / Q000235:genetics / Q000276:immunology; D018797:Cell Cycle Proteins; D002455:Cell Division / Q000235:genetics / Q000276:immunology; D002460:Cell Line; D018925:Chemokines / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D018920:Coculture Techniques; D051546:Cyclooxygenase 2; D016207:Cytokines / Q000096:biosynthesis / Q000235:genetics; D003713:Dendritic Cells / Q000166:cytology / Q000201:enzymology / Q000276:immunology* / Q000378:metabolism; D015536:Down-Regulation / Q000235:genetics / Q000276:immunology; D020869:Gene Expression Profiling / Q000379:methods; D006801:Humans; D007108:Immune Tolerance / Q000235:genetics; D007527:Isoenzymes / Q000096:biosynthesis / Q000235:genetics; D008213:Lymphocyte Activation / Q000235:genetics; D008565:Membrane Proteins; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D011451:Prostaglandin-Endoperoxide Synthases / Q000096:biosynthesis / Q000235:genetics; D014176:Protein Biosynthesis; D054645:Protein Phosphatase 1; D011506:Proteins / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics*; D015398:Signal Transduction / Q000235:genetics / Q000276:immunology; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics; D014407:Tumor Cells, Cultured; D015854:Up-Regulation / Q000235:genetics / Q000276:immunology
chemical_list: D000943:Antigens, Differentiation; D018797:Cell Cycle Proteins; D018925:Chemokines; D016207:Cytokines; D007527:Isoenzymes; D008565:Membrane Proteins; D011506:Proteins; D012333:RNA, Messenger; D014157:Transcription Factors; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; D011451:Prostaglandin-Endoperoxide Synthases; C087519:PPP1R15A protein, human; D054645:Protein Phosphatase 1