Immunocytochemical study of the action of cis-dichlorodiamino platinum on the human metaphase chromosome.
The reaction of cis-dichlorodiamino platinum (cis-Pt) with the human metaphase chromosome was studied using both Giemsa staining and the action of anti-cis-Pt antibody. Two kinds of experiment were performed: (1) cis-Pt was placed in contact with cells in culture (in vivo tests), (2) cis-Pt was placed in contact with chromosomes fixed on slides (in vitro tests). In vivo, the presence of cis-Pt during phase G1 of the cell cycle is important for the reaction; in vitro, competition between cis-Pt and Giemsa for fixation on chromosomal DNA was shown to occur.
Morin-Faure
J
J
Laboratoire d'Histologie-Embryologie-Cytogénétique, U.E.R. Médecine, Clermont-Ferrand/France.
Marcollet
M
M
D000970:Antineoplastic Agents / Q000494:pharmacology*; D001399:Azure Stains / Q000494:pharmacology; D002453:Cell Cycle; D002478:Cells, Cultured; D002875:Chromosomes / Q000187:drug effects*; D002945:Cisplatin / Q000494:pharmacology*; D003432:Cross-Linking Reagents / Q000494:pharmacology*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D008677:Metaphase / Q000187:drug effects*
D000970:Antineoplastic Agents; D001399:Azure Stains; D003432:Cross-Linking Reagents; D002945:Cisplatin