title: Effects of a hemoglobin-based oxygen carrier (HBOC-201) on coagulation testing.
abstract: Polymerized bovine hemoglobin (HBOC-201) is currently under investigation as an alternative to blood banked human red cells. Due to the dark red, hemolyzed appearance of HBOC-201, we sought to describe the effects of HBOC-201 on coagulation analyzers that perform prothrombin times (PT), activated partial thromboplastin times, fibrinogen, and antithrombin. Pooled normal plasma was combined with HBOC-201 to achieve plasma hemoglobin levels of 1.4, 2.6 3.8, 4.8, and 6.2 g/dL. Results for each test from HBOC-201 prepared plasmas were compared to saline matched controls. Two consecutive absolute result differences of > 10% between saline controls and HBOC-201 samples were used for determining interference on test accuracy by the concentration of HBOC-201. Mechanical detection methods (fibrometer, STA, CS-190) and the MDA-180 were less affected by increasing levels of HBOC-201 than optical detection devices for all test parameters.
authors.affiliation: Department of Anesthesiology, University of California Davis Medical Center, 4150 V Street, Suite 1200, Sacramento, CA 95817, USA.
authors.forename: J S
authors.initials: JS
authors.lastname: Jahr
authors.forename: F
authors.initials: F
authors.lastname: Lurie
authors.forename: R
authors.initials: R
authors.lastname: Gosselin
authors.forename: J S
authors.initials: JS
authors.lastname: Lin
authors.forename: L
authors.initials: L
authors.lastname: Wong
authors.forename: E
authors.initials: E
authors.lastname: Larkin
mesh_terms: D001780:Blood Coagulation Tests; D001802:Blood Substitutes / Q000494:pharmacology*; D006454:Hemoglobins / Q000494:pharmacology*; D006801:Humans
chemical_list: D001802:Blood Substitutes; D006454:Hemoglobins; C096798:HBOC 201