Differentially expressed genes in association with in vitro invasiveness of human epithelioid sarcoma.
OBJECTIVE
Differential display reverse transcription polymerase chain reaction (RT-PCR) was performed to identify genes associated with the invasive potential of human epithelioid sarcoma.


METHODS
Two different clonal subpopulations, GRU-1A and GRU-1B, derived from the same human epithelioid sarcoma cell line GRU-1 and known to differ greatly in their invasive potential were compared by means of mRNA fingerprinting.


RESULTS
Using a set of 10 arbitrary upstream primers and nine anchored oligo-dT primers, 22 candidate gene fragments were identified; differential expression was confirmed in four of these fragments by northern blot analysis. At the mRNA level, apoferritin light chain was predominantly expressed by the highly invasive cell line GRU-1A. In contrast, the mitochondrial gene M1, encoding cytochrome c oxidase I, and the TI-227H gene were expressed more strongly by the low invasive cell line GRU-1B. Furthermore, a novel gene fragment was identified and cloned that was preferentially expressed in the low invasive cell line GRU-1B, and therefore might have an inhibitory role in invasion. Consequently, this gene fragment was designated as expressed in low invasive sarcoma cells (ELISC-1).


CONCLUSIONS
A novel gene fragment (ELISC-1) and three known genes were identified as potential regulators of tumour invasiveness. Cloning of the entire sequence of ELISC-1 and subsequent investigations are required to establish its biological role.
Weber
A
A
Institute of Pathology, Heinrich-Heine-University, Moorenstr. 5, D-40225 Duesseldorf, Germany.
Engers
R
R
Nockemann
S
S
Gohr
L L
LL
Zur Hausen
A
A
Gabbert
H E
HE
D000955:Antigens, T-Independent / Q000378:metabolism; D001052:Apoferritins / Q000378:metabolism; D015152:Blotting, Northern; D016172:DNA Fingerprinting; D003576:Electron Transport Complex IV / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D009361:Neoplasm Invasiveness / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012509:Sarcoma / Q000235:genetics*; D014407:Tumor Cells, Cultured
D000955:Antigens, T-Independent; D012333:RNA, Messenger; D001052:Apoferritins; D003576:Electron Transport Complex IV