title: Characterization of human apolipoprotein B100 oligosaccharides in LDL subfractions derived from normal and hyperlipidemic plasma: deficiency of alpha-N-acetylneuraminyllactosyl-ceramide in light and small dense LDL particles.
abstract: The carbohydrate composition of apolipoprotein (apo) B100, particularly its degree of sialylation, may contribute to the atherogenic properties of low-density lipoprotein (LDL). We analyzed LDL apoB100 glycans derived from normolipidemic, hypercholesterolemic, and hypertriglyceridemic diabetic subjects. Using exoglycosidase carbohydrate sequencing and matrix-assisted laser desorption/ionization mass spectrometry to analyze fluorescently labeled oligosaccharides, we report evidence for several carbohydrates not previously identified on apoB100, including truncated complex biantennary N-glycans and hybrid N-glycans. The distribution and diversity of the apoB100 glycans isolated from all individuals was highly conserved. The N-glycan composition of apoB100 derived from five LDL subpopulations (LDL1, d = 1.018-1.023; LDL2, d = 1.023-1.030; LDL3, d = 1.030-1.040; LDL4, d = 1.040-1.051; LDL5, d = 1.051-1.065 g/ml) did not vary in normolipidemic or hypercholesterolemic subjects. Furthermore, we found no evidence for "desialylated" apoB100 glycans in any of the samples analyzed. Analysis of the most abundant LDL ganglioside, alpha-N-acetylneuraminyllactosyl-ceramide, revealed a deficiency in small dense LDL and in the most buoyant subpopulation. These data provide a novel explanation for the apparent deficiency of sialic acid in small dense LDL and indicate that the global apoB100 N-glycan composition is invariable in the patient groups studied.
authors.affiliation: Oxford Glycobiology Institute, University of Oxford, South Parks Road, Oxford OX1 3QU, UK.
authors.forename: B
authors.initials: B
authors.lastname: Garner
authors.forename: D J
authors.initials: DJ
authors.lastname: Harvey
authors.forename: L
authors.initials: L
authors.lastname: Royle
authors.forename: M
authors.initials: M
authors.lastname: Frischmann
authors.forename: F
authors.initials: F
authors.lastname: Nigon
authors.forename: M J
authors.initials: MJ
authors.lastname: Chapman
authors.forename: P M
authors.initials: PM
authors.lastname: Rudd
mesh_terms: D053299:Apolipoprotein B-100; D001055:Apolipoproteins B / Q000737:chemistry*; D002240:Carbohydrate Sequence; D002518:Ceramides / Q000032:analysis*; D006801:Humans; D006949:Hyperlipidemias / Q000097:blood*; D008077:Lipoproteins, LDL / Q000097:blood / Q000737:chemistry*; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000032:analysis*; D011134:Polysaccharides / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D053299:Apolipoprotein B-100; D001055:Apolipoproteins B; D002518:Ceramides; D008077:Lipoproteins, LDL; D009844:Oligosaccharides; D011134:Polysaccharides