title: Behavioral characterization of dopamine D5 receptor null mutant mice.
abstract: To study behavioral functions of the D5 subtype, mice were generated with null mutations in the D5 gene. This 1st behavioral characterization of D5 null mutant mice (D5-/-) indicated normal general health, sensory abilities, and neurological reflexes. Under basal conditions, D5-/- mice were generally normal on locomotor activity, the rotarod test, acoustic startle response, prepulse inhibition, elevated plus-maze, light <--> dark exploration, Morris water maze, and cued and contextual fear conditioning. In the Porsolt forced swim test for antidepressant activity, male D5-/- mice showed lower levels of immobility. D5-/- mice showed some evidence of reduced responses to the hyperactivity-inducing effects of the D1/D5 receptor agonist SKF 81297. The ability of SKF 81297 to disrupt acoustic startle and prepulse inhibition appeared to be attenuated in D5-/- mice. These results suggest that the D5 receptor is not essential for many dopamine-mediated behaviors but may contribute to the pharmacological activation of dopaminergic pathways relevant to exploratory locomotion, startle, and prepulse inhibition.
authors.affiliation: Section on Behavioral Genomics, National Institute of Mental Health, National Institutes of Health, Bethesda, Maryland 20892-1375, USA. aholmes@codon.nih.gov
authors.forename: A
authors.initials: A
authors.lastname: Holmes
authors.forename: T R
authors.initials: TR
authors.lastname: Hollon
authors.forename: T C
authors.initials: TC
authors.lastname: Gleason
authors.forename: Z
authors.initials: Z
authors.lastname: Liu
authors.forename: J
authors.initials: J
authors.lastname: Dreiling
authors.forename: D R
authors.initials: DR
authors.lastname: Sibley
authors.forename: J N
authors.initials: JN
authors.lastname: Crawley
mesh_terms: D000818:Animals; D001143:Arousal / Q000235:genetics / Q000502:physiology; D001522:Behavior, Animal / Q000502:physiology*; D001921:Brain / Q000502:physiology; D005106:Exploratory Behavior / Q000502:physiology; D005260:Female; D008124:Locomotion / Q000502:physiology; D008297:Male; D018782:Maze Learning / Q000502:physiology; D051379:Mice; D018345:Mice, Knockout; D008818:Mice, Neurologic Mutants; D009042:Motivation; D009433:Neural Inhibition / Q000502:physiology; D017447:Receptors, Dopamine D1 / Q000235:genetics* / Q000502:physiology; D050641:Receptors, Dopamine D5; D013216:Reflex, Startle / Q000502:physiology
chemical_list: C493606:Drd5 protein, mouse; D017447:Receptors, Dopamine D1; D050641:Receptors, Dopamine D5