Molecular characterization of DDX26, a human DEAD-box RNA helicase, located on chromosome 7p12.
DEAD-box proteins comprise a family of ATP-dependent RNA helicases involved in several aspects of RNA metabolism. Here we report the characterization of the human DEAD-box RNA helicase DDX26. The gene is composed of 14 exons distributed over an extension of 8,123 bp of genomic sequence and encodes a transcript of 1.8 kb that is expressed in all tissues evaluated. The predicted amino acid sequence shows a high similarity to a yeast DEAD-box RNA helicase (Dbp9b) involved in ribosome biogenesis. The new helicase maps to 7p12, a region of frequent chromosome amplifications in glioblastomas involving the epidermal growth factor receptor (EGFR) gene. Nevertheless, co-amplification of DDX26 with EGFR was not detected in nine tumors analyzed.
Camargo
A A
AA
Instituto Ludwig de Pesquisa sobre o Câncer, São Paulo, SP, Brasil. anamaria@compbio.ludwig.org.br
Nunes
D N
DN
Samaia
H B
HB
Liu
L
L
Collins
V P
VP
Simpson
A J
AJ
Dias-Neto
E
E
D000595:Amino Acid Sequence; D000818:Animals; D002175:Candida / Q000235:genetics; D002897:Chromosomes, Human, Pair 7 / Q000235:genetics*; D017124:Conserved Sequence; D004330:Drosophila / Q000235:genetics; D005784:Gene Amplification; D015870:Gene Expression; D018773:Genes, erbB-1; D005909:Glioblastoma / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D020365:RNA Helicases / Q000737:chemistry* / Q000235:genetics*; D016601:RNA-Binding Proteins; D012269:Ribosomal Proteins; D014407:Tumor Cells, Cultured; D025521:Tumor Suppressor Proteins; D015003:Yeasts / Q000235:genetics
C121427:INTS6 protein, human; D016601:RNA-Binding Proteins; D012269:Ribosomal Proteins; D025521:Tumor Suppressor Proteins; D020365:RNA Helicases