title: Detection of the presenilin 1 COOH-terminal fragment in the extracellular compartment: a release enhanced by apoptosis.
abstract: Mutations in gene encoding presenilin 1 (PS1) are responsible for the majority of familial Alzheimer's disease (FAD) cases. We studied PS1 localization in HEK293 cells and in primary neurons obtained from rat cortex and hippocampus. We first demonstrated that PS1-CTF, but neither PS1-FL nor PS1-NTF, is released into the medium as a soluble and membrane-associated form. After induction of apoptosis with staurosporine (Sts), we observed a dramatic increase in the level of PS1-CTF in the medium, both in HEK293 and in primary neurons. Immunocytochemical analysis suggested that the release of PS1-CTF might occur via membrane shedding. Abeta(1-42) treatment reduced PS1-CTF extracellular levels. This decrease was strongly associated to an impaired secretion of sAPP fragments, thus suggesting a role of PS1-CTF in the control of trafficking and generation of APP fragments.
authors.affiliation: Scientific Institute IRCCS "Centro San Giovanni di Dio-Fatebenefratelli", Brescia, 25123, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Benussi
authors.forename: A
authors.initials: A
authors.lastname: Alberici
authors.forename: M
authors.initials: M
authors.lastname: Mayhaus
authors.forename: U
authors.initials: U
authors.lastname: Langer
authors.forename: R
authors.initials: R
authors.lastname: Ghidoni
authors.forename: F
authors.initials: F
authors.lastname: Mazzoli
authors.forename: F
authors.initials: F
authors.lastname: Nicosia
authors.forename: L
authors.initials: L
authors.lastname: Barbiero
authors.forename: G
authors.initials: G
authors.lastname: Frisoni
authors.forename: O
authors.initials: O
authors.lastname: Zanetti
authors.forename: L
authors.initials: L
authors.lastname: Gasparini
authors.forename: R M
authors.initials: RM
authors.lastname: Nitsch
authors.forename: G
authors.initials: G
authors.lastname: Binetti
mesh_terms: D000544:Alzheimer Disease / Q000378:metabolism; D016229:Amyloid beta-Peptides / Q000494:pharmacology; D000818:Animals; D017209:Apoptosis; D015153:Blotting, Western; D002460:Cell Line; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005109:Extracellular Matrix / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D008565:Membrane Proteins / Q000737:chemistry*; D008856:Microscopy, Fluorescence; D009474:Neurons / Q000378:metabolism; D010446:Peptide Fragments / Q000494:pharmacology; D010455:Peptides / Q000494:pharmacology; D053764:Presenilin-1; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D051381:Rats; D017208:Rats, Wistar; D019311:Staurosporine / Q000494:pharmacology
chemical_list: D016229:Amyloid beta-Peptides; D004791:Enzyme Inhibitors; D008565:Membrane Proteins; C508853:PSEN1 protein, human; D010446:Peptide Fragments; D010455:Peptides; D053764:Presenilin-1; C075222:amyloid beta-protein (1-42); D019311:Staurosporine