Acute stress-induced increases in thalamic CRH mRNA are blocked by repeated stress exposure.
Corticotropin-releasing hormone (CRH) coordinates multiple aspects of the stress response. Recently, CRH mRNA has been identified in two regions of the thalamus: the posterior nuclear group (Po), and a region located at the interface of the central medial and ventral posteromedial nucleus (parvicellular part) (CM-VPMpc). Previous studies demonstrated that in both regions CRH mRNA increases following 1 h of restraint stress, suggesting involvement of thalamic CRH in processing somatosensory and visceral information related to stress. The current study was proposed to further understand the effects of repeated and acute restraint stress on levels of thalamic CRH mRNA. Adult male rats were assigned to one of four groups in a 2 (repeated stress, no repeated) x2 (acute, no acute) design. Brain sections were processed for CRH mRNA in situ hybridization. ANOVA revealed no main effects of acute or repeated stress in either thalamic region. However, significant interactions between acute and repeated stress for levels of CRH mRNA were found for both regions of the thalamus. Compared to the no stress condition, acute restraint significantly increased CRH mRNA in the Po (39%) and the CM-VPMpc (32%). Repeated restraint did not alter baseline CRH mRNA levels, but blocked the acute restraint-induced effects. Thus, while acute stress increases levels of thalamic CRH mRNA, repeated exposure to the same stressor is without effect and prevents the acute response. These findings add to data establishing a role for thalamic CRH in the stress response and suggest a mechanism that may underlie habituation to repeated stress exposure.
Hsu
D T
DT
Department of Psychiatry, University of Wisconsin, 6001 Research Park Blvd., Madison, WI 53719, USA. dthsu@students.wisc.edu
Lombardo
K A
KA
Bakshi
V P
VP
Balachandran
J S
JS
Roseboom
P H
PH
Kalin
N H
NH
D000208:Acute Disease; D000818:Animals; D003346:Corticotropin-Releasing Hormone / Q000235:genetics*; D005786:Gene Expression Regulation / Q000502:physiology; D020646:Intralaminar Thalamic Nuclei / Q000166:cytology / Q000378:metabolism; D008297:Male; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D010146:Pain / Q000378:metabolism / Q000503:physiopathology; D020656:Posterior Thalamic Nuclei / Q000166:cytology / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012149:Restraint, Physical; D013312:Stress, Physiological / Q000235:genetics / Q000378:metabolism* / Q000503:physiopathology; D013788:Thalamus / Q000166:cytology / Q000378:metabolism*; D014110:Touch / Q000502:physiology; D015854:Up-Regulation / Q000235:genetics*; D020651:Ventral Thalamic Nuclei / Q000166:cytology / Q000378:metabolism
D012333:RNA, Messenger; D003346:Corticotropin-Releasing Hormone