[The role of beta-ray induced fibroblasts apoptosis on the inhibition of hypertrophic scar].
OBJECTIVE
To investigate the role of beta-ray induced apoptosis of the fibroblasts on the inhibition of hypertrophic scar.


METHODS
A series of patients with hypertrophic scar were grouply treated with a certain dose beta-ray. A liopic sample was taken in each patients. Its was then examined with MTT[3-(4,5-dimethylthiazol.-2-yl)-2,5-dipheny1 terazolium-bromide] technique for deciding the inhibition rate of the fibroblasts, with electron microscope technique, DNA agarose gel electrophoresis and flow cytometry for evaluating the morphological and biochemical characteristics of the fibroblast.


RESULTS
The beta-ray radiation resulted in significant inhibition of the fibroblast. The features of fibroblast apoptosis(e.g.: apoptotic bodies, DNA ladders band and hypodiploid DNA peak) could be seen in the group with lower does (10 Gy). The features of fibroblast necrosis could also be found in the group with higher does(20 Gy).


CONCLUSIONS
The beta-ray radiation could have the effect for inhibiting the proliferation of scar-derived fibroblast, but with dose dependence. It could be used for the treatment and(or) the prevention of hypertrophic scar.
Wang
X
X
Department of Plastic Surgery, Nangfang Hospital, First Military Medical University, Guangzhou 510515, PR China.
Luo
J
J
D017209:Apoptosis / Q000528:radiation effects*; D001610:Beta Particles; D002478:Cells, Cultured; D017439:Cicatrix, Hypertrophic / Q000473:pathology* / Q000517:prevention & control; D005347:Fibroblasts / Q000473:pathology*; D006801:Humans