Assessment of internalization and viability of Porphyromonas gingivalis in KB epithelial cells by confocal microscopy.
Porphyromonas gingivalis (P. gingivalis) is considered to be one of the main periodontal pathogens. The goal of this work was to confirm the ability of P. gingivalis to invade host cells. We detected P. gingivalis inside KB cells by confocal microscopy and analyzed the various aspects of the adherence and internalization process. Lysates of P. gingivalis-infected KB cells were also examined using anaerobic growth techniques. The results showed the viability and ability to replicate, inside the host cells, of the internalized pathogen. The production of vesicles was also tracked for the first time. Confocal microscopy revealed P. gingivalis in a perinuclear position.
Houalet-Jeanne
S
S
Equipe de Biologie Buccale, UPRES-EA 1256, 35000 Rennes, France.
Pellen-Mussi
P
P
Tricot-Doleux
S
S
Apiou
J
J
Bonnaure-Mallet
M
M
D004705:Endocytosis / Q000276:immunology*; D004847:Epithelial Cells; D006801:Humans; D018613:Microscopy, Confocal / Q000379:methods; D016966:Porphyromonas gingivalis / Q000254:growth & development / Q000276:immunology*; D014407:Tumor Cells, Cultured