Stability of sufentanil in human plasma samples.
The stability of sufentanil in human plasma kept under various storage conditions was investigated. Extraction was performed using solid phase extraction with new mixed-mode cation exchange Oasis MCX columns; quantification was carried out using gas chromatography equipped with a mass spectrometry detector. When plasma was left at 4 degrees C in nonsilanized tubes, concentrations of sufentanil decreased significantly during the first hour. In plasma samples kept at -25 degrees C for 8 hours in nonsilanized glass tubes, a significant decrease of sufentanil concentrations was found, with an average loss of 10.1% of the initial concentration. A significant decrease occurred when plasma was kept in silanized glass tubes for 12 hours at -25 degrees C. The current study emphasizes the importance of sampling and storage conditions for an accurate determination of sufentanil concentration in plasma.
Dufresne
C
C
Department of Clinical Pharmacy, Pharmacokinetics, and Drug Evaluation, Institute of Pharmaceutic and Biologic Sciences, Claude Bernard Lyon 1 University (UCBL), Lyon, France.
Favetta
P
P
Paradis
C
C
Boulieu
R
R
D015760:Alfentanil / Q000097:blood / Q000737:chemistry; D000701:Analgesics, Opioid / Q000097:blood / Q000737:chemistry*; D004349:Drug Packaging; D004355:Drug Stability; D004356:Drug Storage; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D017409:Sufentanil / Q000097:blood / Q000737:chemistry*; D013696:Temperature; D013997:Time Factors
D000701:Analgesics, Opioid; D015760:Alfentanil; D017409:Sufentanil