Detection of virulence associated genes in clinical strains of vibrio mimicus.
A total of 42 clinical strains of Vibrio mimicus were examined for the presence of virulence associated genes toxR, toxS, toxT, tcpP, ctx and tcpA by PCR assay. Almost all strains were shown to have the toxR gene, while the toxS gene was found in 27 strains. On the other hand, five strains possessed both toxT and tcpP genes, but others had neither. Only two strains were positive for amplification of the ctx gene, whereas no PCR product with tcpA primers was detected. The results indicate the incomplete copies of virulence cascade in V mimicus strains. The pathogenesis and epidemic potential of this species is also discussed.
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Faculty of Pharmaceutical Sciences, Okayama University, Japan. bikewei@hotmail.com
Miyoshi
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SI
Tomochika
K I
KI
Shinoda
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D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D002772:Cholera Toxin / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics; D036781:Fimbriae Proteins; D005798:Genes, Bacterial; D006801:Humans; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D014157:Transcription Factors / Q000235:genetics; D014733:Vibrio / Q000235:genetics* / Q000472:pathogenicity*; D014735:Vibrio Infections / Q000382:microbiology*
D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D008565:Membrane Proteins; C097244:TCPP protein, Vibrio cholerae; C085807:TcpA protein, Vibrio cholerae; D014157:Transcription Factors; C499324:toxR protein, Vibrio cholerae; C066719:toxR protein, bacteria; C066828:ToxS protein, Vibrio cholerae; D036781:Fimbriae Proteins; C075626:tcpN protein, Vibrio cholerae; D002772:Cholera Toxin