title: Isolation and amino acid sequence of two new PR-4 proteins from wheat.
abstract: We have purified and characterized two new pathogenesis-related (PR) proteins from wheat belonging to the PR-4 family. We named the proteins wheatwin3 and wheatwin4 in analogy with the previously characterized wheatwin1 and wheatwin2. Their isoelectric points were 7.1 and 8.4, respectively. We determined the complete amino acid sequence of both proteins by a rapid approach based on the knowledge of the primary structures of the homologous wheatwin1 and wheatwin2. Wheatwin3 differs from wheatwin1 in one substitution at position 88, while wheatwin4 differs from wheatwin2 in one substitution at position 78. The secondary structure and solvent accessibility of these residues were determined on the three-dimensional model of wheatwinl. Residue 88 was very accessible and was located in a flexible region. Preliminary results indicate that, like wheatwin1 and wheatwin2, wheatwin3 and wheatwin4 have antifungal activity.
authors.affiliation: Dipartimento di Agrobiologia ed Agrochimica, Universitá della Tuscia, Viterbo, Italy. caruso@unitus.it
authors.forename: C
authors.initials: C
authors.lastname: Caruso
authors.forename: M
authors.initials: M
authors.lastname: Nobile
authors.forename: L
authors.initials: L
authors.lastname: Leonardi
authors.forename: L
authors.initials: L
authors.lastname: Bertini
authors.forename: V
authors.initials: V
authors.lastname: Buonocore
authors.forename: C
authors.initials: C
authors.lastname: Caporale
mesh_terms: D000478:Alkylation; D000595:Amino Acid Sequence; D000935:Antifungal Agents / Q000737:chemistry* / Q000302:isolation & purification; D015151:Immunoblotting; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification*; D017421:Sequence Analysis; D014908:Triticum / Q000737:chemistry*; D014357:Trypsin / Q000378:metabolism
chemical_list: D000935:Antifungal Agents; D010446:Peptide Fragments; D010455:Peptides; D010940:Plant Proteins; C053376:pathogenesis-related proteins, plant; D014357:Trypsin