[Genetic analysis of T1 progeny of transgenic tobacco with metallothionein gene and metallothionein domain mutant alpha alpha gene].
The chimeric gene containing a cloned mouse metallothionein processed gene or a cloned mouse metallothionein domain mutant alpha alpha gene was respectively introduced into tobacco (Nicotiana tobacum L. cv. NC89) on a disarmed Ti-plasmid of Agrobacterium tumefaciens. T1 seeds from self-fertilized transgenic tobacco were germinated on media containing cadmium or herbicide PPT. The PPT tolerance trait followed Mendelian inheritance and co-segregated with heavy metal tolerance. Meanwhile Southern blot and Western blot verified the existence of the MT gene and alpha alpha mutant gene in the T1 generation plants which keep tolerance to heavy metal. All the results demonstrated the stable integration and inheritance of exotic genes. In addition, assay of the root length and fresh weight of T1 seedlings indicate that transgenic tobacco plants with alpha alpha mutant gene still have a little higher tolerance than that with matural MT gene.
Zhang
X Y
XY
National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, China.
Zuo
X F
XF
Shan
L
L
Ru
B G
BG
D000818:Animals; D006540:Herbicides / Q000633:toxicity; D008668:Metallothionein / Q000235:genetics*; D008670:Metals / Q000633:toxicity*; D051379:Mice; D009154:Mutation; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D014026:Nicotiana / Q000187:drug effects / Q000235:genetics*
D006540:Herbicides; D008670:Metals; D008668:Metallothionein