title: Potential role of endogenous brain-derived neurotrophic factor in long-term neuronal reorganization of the superior colliculus after bilateral visual deprivation.
abstract: The role of the brain-derived neurotrophic factor (BDNF), the BDNF receptor (TrkB), and the glutamic acid decarboxylase (GAD67) after neonatal, bilateral nerve deafferentiation during postnatal development was investigated in the rat superior colliculus (SC). BDNF and GAD67 mRNA expression were significantly increased in optic (Op) and intermediate gray (InG) layers at 5, 8, 15, and 21 days after birth, but not in adult animals. However, TrkB mRNA expression was not modified at any time tested. At 15 days, where changes in BDNF and GAD67 mRNAs were more evident, an upregulation of the NMDAR(1A) mRNA glutamate receptor in the Op and InG, a modification in the pattern of synaptic zinc in the superficial layers of SC, and an increased synaptophysin immunoreactivity in the Op was found. This indicates the existence of a synergic mechanism between BDNF and NMDA to determine refinement of connections after the loss of visual input in SC.
authors.affiliation: Departamento de Bioquímica, Bromatología y Toxicología, Facultad de Farmacia, Universidad de Sevilla, Sevilla, 41012, Spain.
authors.forename: M L
authors.initials: ML
authors.lastname: Vizuete
authors.forename: J L
authors.initials: JL
authors.lastname: Venero
authors.forename: C
authors.initials: C
authors.lastname: Vargas
authors.forename: M
authors.initials: M
authors.lastname: Revuelta
authors.forename: A
authors.initials: A
authors.lastname: Machado
authors.forename: J
authors.initials: J
authors.lastname: Cano
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001766:Blindness / Q000503:physiopathology*; D019208:Brain-Derived Neurotrophic Factor / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D015353:Eye Enucleation; D005968:Glutamate Decarboxylase / Q000096:biosynthesis / Q000235:genetics; D017403:In Situ Hybridization; D007527:Isoenzymes / Q000096:biosynthesis / Q000235:genetics; D008297:Male; D016202:N-Methylaspartate / Q000502:physiology; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D009473:Neuronal Plasticity / Q000235:genetics* / Q000502:physiology; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D020813:Receptor, trkB / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D016194:Receptors, N-Methyl-D-Aspartate / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D015398:Signal Transduction; D013477:Superior Colliculi / Q000378:metabolism*; D013569:Synapses / Q000378:metabolism / Q000648:ultrastructure; D016708:Synaptophysin / Q000032:analysis; D015032:Zinc / Q000032:analysis
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D005968:Glutamate Decarboxylase; D007527:Isoenzymes; D016202:N-Methylaspartate; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D020813:Receptor, trkB; D016194:Receptors, N-Methyl-D-Aspartate; D016708:Synaptophysin; D015032:Zinc; C402565:glutamate decarboxylase 1