title: Early diagnosis of paediatric HIV-1 infection among African breast-fed children using a quantitative plasma HIV RNA assay.
abstract: OBJECTIVE
  To evaluate the performance of a quantitative plasma HIV-1 RNA assay for HIV infection diagnosis among African breast-fed children.
  
  
  METHODS
  Serial plasma specimens collected in the first week, at day 45-90, 6 months and 9-12 months of age from HIV-exposed children born to HIV-1-infected women enrolled in the DITRAME ANRS 049a perinatal intervention trial (Abidjan, Côte d'Ivoire) were tested for HIV-1 plasma RNA using a branched DNA (bDNA) assay. Sensitivity and specificity of this RNA test were assessed in comparison with a qualitative DNA polymerase chain reaction (PCR) performed on the same blood samples and allowing a reliable detection of the predominant subtype A.
  
  
  RESULTS
  Among 91 samples from 53 infected children which tested positive by DNA PCR, the sensitivity of the bDNA test was 100% [95% confidence interval (CI), 96.0-100.0] at < or = 8 days (n = 19), 6-12 weeks (n = 43), 6 months (n = 26), and 9-12 months (n = 3). The median plasma HIV-1 RNA viral load ranged from 242 000 copies/ml at < or = 8 days to more than 500 000 copies/ml at day 45-90 and at 6 months. Of 106 specimens from 106 uninfected children who were DNA PCR- negative at month 3 or 6 of age, HIV-1 RNA was undetectable in 103, yielding an overall specificity for the bDNA test of 97.2% (95% CI, 92.0-99.4). The viral load in the three remaining samples with false-positive results was low (410, 937 and 3752 copies/ml, respectively).
  
  
  CONCLUSIONS
  The quantitative bDNA assay appears a suitable tool for early, reliable and easy diagnosis of paediatric HIV-1 infection among a population of African breast-fed children.
authors.affiliation: CeDReS, Programme PAC-CI, Abidjan, Côte d'Ivoire. pacci@aviso.ci
authors.forename: F
authors.initials: F
authors.lastname: Rouet
authors.forename: C
authors.initials: C
authors.lastname: Montcho
authors.forename: C
authors.initials: C
authors.lastname: Rouzioux
authors.forename: V
authors.initials: V
authors.lastname: Leroy
authors.forename: P
authors.initials: P
authors.lastname: Msellati
authors.forename: J B
authors.initials: JB
authors.lastname: Kottan
authors.forename: B
authors.initials: B
authors.lastname: You
authors.forename: I
authors.initials: I
authors.lastname: Viho
authors.forename: F
authors.initials: F
authors.lastname: Dabis
mesh_terms: D000349:Africa; D019380:Anti-HIV Agents / Q000627:therapeutic use; D021121:Branched DNA Signal Amplification Assay / Q000379:methods*; D001942:Breast Feeding; D005260:Female; D015483:HIV Antibodies / Q000097:blood; D015658:HIV Infections / Q000175:diagnosis* / Q000821:virology; D015497:HIV-1 / Q000302:isolation & purification*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000097:blood*; D018894:Reverse Transcriptase Inhibitors / Q000627:therapeutic use; D012680:Sensitivity and Specificity; D015215:Zidovudine / Q000627:therapeutic use
chemical_list: D019380:Anti-HIV Agents; D015483:HIV Antibodies; D012367:RNA, Viral; D018894:Reverse Transcriptase Inhibitors; D015215:Zidovudine