title: Difference in cholesterol-binding and cytolytic activities between listeriolysin O and seeligeriolysin O: a possible role of alanine residue in tryptophan-rich undecapeptide.
abstract: We have constructed recombinant listeriolysin O (rLLO) and seeligeriolysin O (rLSO) from Listeria monocytogenes and Listeria seeligeri, respectively. In hemolysis and cholesterol-binding assays, the specific activity of recombinant toxin was lower for LSO as compared to LLO. To understand the molecular basis of this difference, in particular with respect to the conserved Trp-rich undecapeptide, a naturally occurring Ala to Phe substitution in LSO was introduced into rLLO. The rLLO:A488F hemolysin exhibited a reduced activity in both hemolysis and cholesterol-binding. The reverse mutation, inserted into rLSO, also increased the hemolytic activity of this mutant LSO. These results suggested that the natural replacement of Ala to Phe is involved in the weak cytolytic activity of LSO.
authors.affiliation: Department of Microbiology, Kyoto University Graduate School of Medicine, Yoshidakanoe-cho, Sakyo-ku, Kyoto 606-8501, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Ito
authors.forename: I
authors.initials: I
authors.lastname: Kawamura
authors.forename: C
authors.initials: C
authors.lastname: Kohda
authors.forename: H
authors.initials: H
authors.lastname: Baba
authors.forename: T
authors.initials: T
authors.lastname: Kimoto
authors.forename: I
authors.initials: I
authors.lastname: Watanabe
authors.forename: T
authors.initials: T
authors.lastname: Nomura
authors.forename: M
authors.initials: M
authors.lastname: Mitsuyama
mesh_terms: D000409:Alanine / Q000737:chemistry; D000818:Animals; D001427:Bacterial Toxins; D002784:Cholesterol / Q000378:metabolism*; D006360:Heat-Shock Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000633:toxicity; D006460:Hemolysin Proteins / Q000235:genetics / Q000378:metabolism / Q000633:toxicity; D006461:Hemolysis; D008087:Listeria / Q000378:metabolism; D008089:Listeria monocytogenes / Q000378:metabolism; D009154:Mutation; D010455:Peptides / Q000737:chemistry; D011994:Recombinant Proteins / Q000378:metabolism; D012756:Sheep; D014364:Tryptophan / Q000737:chemistry
chemical_list: D001427:Bacterial Toxins; D006360:Heat-Shock Proteins; D006460:Hemolysin Proteins; D010455:Peptides; D011994:Recombinant Proteins; D014364:Tryptophan; D002784:Cholesterol; D000409:Alanine; C038705:hlyA protein, Listeria monocytogenes