title: Protein estimation directly from SDS-PAGE loading buffer for standardization of samples from cell lysates or tissue homogenates before Western blot analysis.
authors.affiliation: Université Laval, Sainte-Foy, QC, Canada.
authors.forename: P
authors.initials: P
authors.lastname: Chapdelaine
authors.forename: K
authors.initials: K
authors.lastname: Vignola
authors.forename: M A
authors.initials: MA
authors.lastname: Fortier
mesh_terms: D000580:Amido Black; D015153:Blotting, Western; D002021:Buffers; D018929:Cell Culture Techniques; D002725:Chloroform; D003101:Collodion; D004396:Coloring Agents; D046508:Culture Techniques; D051546:Cyclooxygenase 2; D004229:Dithiothreitol; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D004717:Endometrium / Q000201:enzymology; D005260:Female; D006801:Humans; D007202:Indicators and Reagents; D007527:Isoenzymes / Q000032:analysis*; D008565:Membrane Proteins; D000432:Methanol; D010664:Phenylmethylsulfonyl Fluoride; D011451:Prostaglandin-Endoperoxide Synthases / Q000032:analysis*; D011506:Proteins / Q000032:analysis* / Q000302:isolation & purification; D014867:Water
chemical_list: D002021:Buffers; D004396:Coloring Agents; D007202:Indicators and Reagents; D007527:Isoenzymes; D008565:Membrane Proteins; D011506:Proteins; D014867:Water; D010664:Phenylmethylsulfonyl Fluoride; D002725:Chloroform; D003101:Collodion; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; D011451:Prostaglandin-Endoperoxide Synthases; D000580:Amido Black; D004229:Dithiothreitol; D000432:Methanol