title: L-isoform but not S-isoform of myelin associated glycoprotein promotes neurite outgrowth of mouse cerebellar neurons.
abstract: Myelin associated glycoprotein (MAG) has growth promoting effect on mouse cerebellar neurons. In the present study, we examined which isoform of MAG has the effect. cDNA for L-MAG and S-MAG was stably transfected into BALB/c 3T3 cells, on which cerebellar neurons were cultured. The neurons were stained with antibody against microtubule-associated protein-2. Neurites of the neurons cultured on cells expressing L-MAG extended significantly further than those cultured on cells expressing S-MAG or on control cells. Therefore, intracellular domain of MAG may have the potential to affect MAG-neurite interaction.
authors.affiliation: Department of Immunology, National Institute of Neuroscience, National Center of Neurology on Psychiatry, 4-1-1 Ogawa-higashi, Kodaira, Tokyo 187-8502, Japan.
authors.forename: C
authors.initials: C
authors.lastname: Shimizu-Okabe
authors.forename: Y
authors.initials: Y
authors.lastname: Matsuda
authors.forename: H
authors.initials: H
authors.lastname: Koito
authors.forename: S
authors.initials: S
authors.lastname: Yoshida
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002452:Cell Count; D002454:Cell Differentiation / Q000187:drug effects* / Q000502:physiology; D048429:Cell Size / Q000235:genetics; D002478:Cells, Cultured / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D002531:Cerebellum / Q000166:cytology / Q000187:drug effects* / Q000254:growth & development; D015870:Gene Expression / Q000502:physiology; D005822:Genetic Vectors; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D018992:Myelin-Associated Glycoprotein / Q000235:genetics / Q000378:metabolism*; D016501:Neurites / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D017434:Protein Structure, Tertiary / Q000502:physiology; D014162:Transfection
chemical_list: D008869:Microtubule-Associated Proteins; D018992:Myelin-Associated Glycoprotein; D020033:Protein Isoforms