title: Reduction of colonic mucus by repeated short-term stress enhances experimental colitis in rats.
abstract: The role of stress in inflammatory bowel disease remains debated and few studies have tested the role of stress in conjunction with experimental animal models of colitis. In this investigation we tested the hypothesis that cold-restraint stress would adversely effect the severity of 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced colitis in rats, and examined mechanisms for the response. Results indicated that increasing intermittent prior exposures to stress significantly enhanced TNBS-induced colitis severity. An associated stress-induced decrease in colonic mucin glycoprotein content, reduction in goblet cells, and histochemical mucin suggested reduced mucin was a pathogenetic factor. Myeloperoxidase content increased and mast cell counts in the colon decreased but colonic permeability only temporarily increased with increasing stress exposure. Prior adrenalectomy or administration of an adrenergic blocking agent did not prevent the colonic changes to stress, but mast cell stabilization or inhibition of cholinergic pathways reduced the stress-induced colonic changes.
authors.affiliation: Department of Biomedical Sciences and Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA. carlsan@vt.edu
authors.forename: C J
authors.initials: CJ
authors.lastname: Pfeiffer
authors.forename: B
authors.initials: B
authors.lastname: Qiu
authors.forename: S K
authors.initials: SK
authors.lastname: Lam
mesh_terms: D000315:Adrenalectomy; D000818:Animals; D003092:Colitis / Q000097:blood / Q000150:complications* / Q000473:pathology / Q000503:physiopathology*; D003106:Colon / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D003345:Corticosterone / Q000097:blood; D005730:Ganglionic Blockers / Q000494:pharmacology; D018738:Hexamethonium / Q000494:pharmacology; D007665:Ketotifen / Q000494:pharmacology; D008297:Male; D009077:Mucins / Q000378:metabolism; D009093:Mucus / Q000378:metabolism*; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013312:Stress, Physiological / Q000150:complications* / Q000378:metabolism*
chemical_list: D005730:Ganglionic Blockers; D009077:Mucins; D018738:Hexamethonium; D009195:Peroxidase; D003345:Corticosterone; D007665:Ketotifen