Effect of SpeB and EndoS from Streptococcus pyogenes on human immunoglobulins.
Streptococcus pyogenes secretes a specific immunoglobulin G (IgG)-protease, SpeB, as well as the IgG glycan-hydrolyzing enzyme EndoS. Here we show that SpeB also degrades IgA, IgM, IgD, and IgE. We also show that EndoS only hydrolyzes the glycan moiety on native but not denatured IgG. Thus, SpeB has a broad immunoglobulin-degrading activity, while EndoS is highly specific for IgG.
Collin
M
M
Department of Cell and Molecular Biology, Section for Molecular Pathogenesis, Lund University, Lund, Sweden. mattias.collin@medkem.lu.se
Olsén
A
A
D000907:Antibodies, Bacterial / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D003546:Cysteine Endopeptidases / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000378:metabolism*; D006801:Humans; D007070:Immunoglobulin A / Q000378:metabolism; D007072:Immunoglobulin D / Q000378:metabolism; D007073:Immunoglobulin E / Q000378:metabolism; D007074:Immunoglobulin G / Q000378:metabolism; D007075:Immunoglobulin M / Q000378:metabolism; D007136:Immunoglobulins / Q000378:metabolism*; D013297:Streptococcus pyogenes / Q000201:enzymology*; D013379:Substrate Specificity
D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D007070:Immunoglobulin A; D007072:Immunoglobulin D; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007136:Immunoglobulins; D007073:Immunoglobulin E; D006026:Glycoside Hydrolases; C431325:NDOS protein, Streptococcus pyogenes; D003546:Cysteine Endopeptidases; C042477:streptopain