title: Preventing mediastinal shift after pneumonectomy impairs regenerative alveolar tissue growth.
abstract: To examine the effects of mechanical lung strain on regenerative growth of alveolar septal tissue after pneumonectomy (PNX), we replaced the right lungs of adult dogs with a custom-shaped inflatable silicone prosthesis. The prosthesis was either inflated (Inf) to maintain the mediastinum at the midline or deflated to allow mediastinal shift. The animals were euthanized approximately 15 mo later, and the lungs were fixed at a constant distending pressure. With the Inf prostheses, lung expansion, alveolar septal tissue volumes, surface areas, and diffusing capacity of the tissue-plasma barrier were significantly lower than with the deflated prostheses; the expected post-PNX tissue responses were impaired by 30-60%. Capillary blood volume was significantly higher with Inf prostheses, consistent with microvascular congestion. Measurements in the Inf group remained consistently and significantly higher than those expected for a normal left lung, indicating persistence of partial compensation. In one dog, delayed deflation of the prosthesis 9-10 mo after PNX led to vigorous lung expansion and septal tissue growth, particularly of type II epithelial cells. We conclude that mechanical lung strain is a major signal for regenerative lung growth; however, other signals are also implicated, accounting for a significant fraction of the compensatory response to PNX.
authors.affiliation: Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, Texas 75390-9034, USA. Connie.Hsia@utsouthwestern.edu
authors.forename: C C
authors.initials: CC
authors.lastname: Hsia
authors.forename: E Y
authors.initials: EY
authors.lastname: Wu
authors.forename: E
authors.initials: E
authors.lastname: Wagner
authors.forename: E R
authors.initials: ER
authors.lastname: Weibel
mesh_terms: D000818:Animals; D004285:Dogs; D008168:Lung / Q000737:chemistry / Q000502:physiology*; D008176:Lung Volume Measurements; D008482:Mediastinum / Q000033:anatomy & histology*; D010100:Oxygen / Q000378:metabolism; D011013:Pneumonectomy; D019736:Prostheses and Implants; D011650:Pulmonary Alveoli / Q000502:physiology*; D012038:Regeneration
chemical_list: D010100:Oxygen