[Detection of the wheat salt-tolerant-mutant using PCR-SSCP combining with direct sequenceing].
gf-2.8 is a gene located on the chromosomal homologous group 4, which was reported to have relationship with salt tolerance of wheat. A pair of primers were designed to amplify the coding region of gf-2.8 in the two salt-tolerant mutants and their parents. Agarose gel electrophoresis showed that the 685 bp band was amplified among all the materials. SSCP analysis suggested that 974915 (one of the mutants) was different from the other materials. The sequencing results showed that Jimai 24 and its salt-tolerant mutant 8901-17 had the same sequence as published, which indicated that the mutation site of 8901-17 was not on the gf-2.8 gene. However, there were at least two single base mutations in the gf-2.8 of 974915, one of which caused the alternation of amino acid, this mutation occurred in the conservative region of gf-2.8.
Wang
C T
CT
Hebei Normal University, Life and Science Department, Shijiazhuang 050016, China.
Huang
Z J
ZJ
He
C F
CF
Bi
C L
CL
Shen
Y Z
YZ
D001483:Base Sequence; D008969:Molecular Sequence Data; D009154:Mutation; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000494:pharmacology*; D014908:Triticum / Q000235:genetics*
D012965:Sodium Chloride