[The first isolation of Borrelia burgdorferi sensu stricto in Russia].
18 Borrelia isolates obtained from adult ticks of the Ixodes ricinus species, collected from different plants in April 2000 in the Khostinsk region of the Krasnodar Territory in the vicinity of the health resort of Matsesta not far from Sochi, were identified by means of PCR and the analysis of polymorphism of the restriction fragments of ribosomal rrf-rrl spacer amplicon. Among them, in addition to Borrelia species, found in Russia earlier (B. garinii, B. afzelii, B. valaisiana and B. lusitaniae), the classical causative agent of Lyme borreliosis (B. burgdorferi sensu stricto) was detected for the first time. The isolated strain (lr-4721) is now kept in the collection of the Borreliosis Center of the Ministry of Health of Russia (the Laboratory of Infections Vectors at the Gamaleya Research Institute of Epidemiology and Microbiology). The probable role of this infective agent in infectious pathology in Russia is discussed.
Gorelova
N B
NB
Gamaleya Research Institute of Epidemiology and Microbiology, Moscow, Russia.
Korenberg
E I
EI
Postic
D
D
Iunicheva
Iu V
IuV
Riabova
T E
TE
D000818:Animals; D025065:Borrelia burgdorferi / Q000235:genetics / Q000302:isolation & purification*; D018884:Ixodes / Q000382:microbiology; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012426:Russia