Olfaction in the gypsy moth, Lymantria dispar: effect of pH, ionic strength, and reductants on pheromone transport by pheromone-binding proteins.
The pheromone-binding proteins (PBPs) are 16-kDa abundant proteins in specialized olfactory hairs in insects. The mechanism by which the PBPs remove the pheromone from the inner surface of sensory hairs and deliver it to the sensory cell remains unclear. Existing qualitative models postulate that pheromone is released near the dendrite by a decrease in pH or by a reduced form of the PBP. This study focuses on the two PBPs from the gypsy moth and the enantiomers of the pheromone cis-2-methyl-7,8-epoxyoctadecane. The pH dependence of pheromone binding has revealed three ionizations that are important. The type of ligand influences two of these ionizations. We propose that the (-)-enantiomer of the pheromone interacts with one of the ionizable residues on the protein while the (+)-enantiomer does not. Simultaneous variation of pH and KCl concentration in the physiological range or reduction of disulfide bridges does not change the affinity of PBP for pheromone. We propose a revised model of pheromone transport from the inner surface of the sensory hair to the sensory neuron.
Kowcun
A
A
Department of Chemistry, Simon Fraser University, Burnaby, British Columbia V5A 1S6, Canada.
Honson
N
N
Plettner
E
E
D000818:Animals; D001692:Biological Transport; D002352:Carrier Proteins / Q000378:metabolism*; D004058:Diffusion; D004926:Escherichia coli / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D019476:Insect Proteins; D007477:Ions; D007915:Lepidoptera / Q000378:metabolism*; D008956:Models, Chemical; D010675:Pheromones / Q000378:metabolism* / Q000502:physiology*; D011188:Potassium / Q000378:metabolism; D011189:Potassium Chloride / Q000494:pharmacology; D011485:Protein Binding; D021381:Protein Transport; D012492:Salts / Q000494:pharmacology; D012903:Smell
D002352:Carrier Proteins; D019476:Insect Proteins; D007477:Ions; D010675:Pheromones; D012492:Salts; C403713:pheromone binding protein, insect; D011189:Potassium Chloride; D011188:Potassium