[The effects of PDGF-AB on pro alpha 1 (III). mRNA expression of fibroblasts].
OBJECTIVE
To investigate the mechanism of PDGF-AB to promote wound healing and its role during the process of scar formation.


METHODS
In vitro cultured fibroblasts of human normal skin and hypertrophic scar were treated with PDGF-AB. The expression of Pro alpha 1 (III) mRNA of the fibroblasts was evaluated by in situ hybridization technique.


RESULTS
PDGF-AB could increase Pro alpha 1 (III) mRNA expression of fibroblasts of both normal skin and hypertrophic scar in a dose-dependent manner. However, the effect of PDGF-AB on hypertrophic scar fibroblasts was stronger.


CONCLUSIONS
PDGF-AB may promote wound healing by stimulating extracellular matrix synthesis, e.g. collagen. PDGF-AB may also play a role in scar formation through collagen deposition.
Dong
M
M
Department of Burn Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, PR China.
Chen
B
B
Xu
M
M
D015415:Biomarkers; D002921:Cicatrix / Q000378:metabolism*; D017439:Cicatrix, Hypertrophic / Q000378:metabolism*; D003094:Collagen / Q000096:biosynthesis*; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology*; D011347:Procollagen / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011994:Recombinant Proteins / Q000494:pharmacology; D012867:Skin / Q000378:metabolism*
D015415:Biomarkers; D010982:Platelet-Derived Growth Factor; D011347:Procollagen; D012333:RNA, Messenger; D011994:Recombinant Proteins; C095477:platelet-derived growth factor AB; D003094:Collagen