title: Potassium recycling pathways in the human cochlea.
abstract: OBJECTIVE
  Potential pathways for recycling potassium (K+) used in the maintenance of inner ear electrochemical gradients have been elucidated in animal models. However, little is known about K+ transport in the human cochlea. This study was designed to characterize putative K+ recycling pathways in the human ear and to determine whether observations from animal models can be extrapolated to humans.
  
  
  METHODS
  A prospective laboratory study using an immunohistochemical approach to analyze the distribution of key ion transport mediators in the human cochlea.
  
  
  METHODS
  Human temporal bones were fixed in situ within 1 to 6 hours of death and subsequently harvested at autopsy. Decalcification was accomplished with the aid of microwaving. Immunohistochemical staining was then performed to define the presence and cell type-specific distribution of Na,K-ATPase, sodium-potassium-chloride cotransporter (NKCC), and carbonic anhydrase (CA) in the inner ear.
  
  
  RESULTS
  Staining patterns visualized in the human cochlea closely paralleled those seen in other species. Anti-Na,K-ATPase stained strongly the basolateral plasma membrane of strial marginal cells and nerve endings underlying hair cells. This antibody also localized Na,K-ATPase to type II, type IV, and type V fibrocytes in the spiral ligament and in limbal fibrocytes. NKCC was present in the basolateral membrane of strial marginal cells as well as in type II, type V, and limbal fibrocytes. Immunoreactive carbonic anhydrase was present in type I and type III fibrocytes and in epithelial cells lining Reissner's membrane and the spiral prominence.
  
  
  CONCLUSIONS
  The distribution of several major ion transport proteins in the human cochlea is similar but not identical to that described in various rodent models. These results support the presence of a complex system for recycling and regulating K+ homeostasis in the human cochlea, similar to that described in other mammalian species.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Medical University of South Carolina, Charleston, South Carolina 29425, USA. weberp@musc.edu
authors.forename: P C
authors.initials: PC
authors.lastname: Weber
authors.forename: C D
authors.initials: CD
authors.lastname: Cunningham
authors.forename: B A
authors.initials: BA
authors.lastname: Schulte
mesh_terms: D000818:Animals; D001344:Autopsy; D002256:Carbonic Anhydrases / Q000378:metabolism; D002712:Chlorides / Q000378:metabolism; D003051:Cochlea / Q000378:metabolism*; D007758:Ear, Inner / Q000378:metabolism; D005849:Gerbillinae; D006652:Histological Techniques; D006706:Homeostasis; D006801:Humans; D007150:Immunohistochemistry; D017136:Ion Transport; D011188:Potassium / Q000378:metabolism*; D011446:Prospective Studies; D051381:Rats; D012964:Sodium / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism
chemical_list: D002712:Chlorides; D012964:Sodium; D002256:Carbonic Anhydrases; D000254:Sodium-Potassium-Exchanging ATPase; D011188:Potassium