A high performance liquid chromatographic method for the estimation of diethylcarbamazine content in medicated salt samples.
A simple and reproducible method for the estimation of diethylcarbamazine citrate (DEC) by high performance liquid chromatography (HPLC) in DEC-medicated salt was developed. HPLC analysis was conducted with a mobile phase containing acetonitrile/phosphate buffer (20 mM KH(2)PO(4,) adjusted to pH 3.2 with 10% ortho-phosphoric acid) in the ratio of 1:9 and at a flow rate of 1.5 ml/min. A Phenomenex C8 column (15 cmx4.6 mm) of 5 microm particle size was used for the analysis. Analysis was done at UV 210 nm, 0.02 a.u.f. and 40 degrees C. The coefficient of variation was <10% in the range of 1-25 microg/ml and the minimum detectable level was 0.5 microg/ml. The quality of DEC-medicated salt prepared by two methods was analyzed by using the HPLC method. In spray drying method, 29 and 71% of the samples and in rotating drum method, 9 and 12% of samples were found to contain DEC at 0.15-0.25% and >0.25%, respectively. Thus, this quick and simple HPLC method for the estimation of DEC could play a vital role in checking the quality of the DEC medicated salt used for the control of filariasis.
Mathew
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Vector Control Research Centre (ICMR), Indira Nagar, Pondicherry 605006, India. mosquito@md5.vsnl.net.in
Kalyanasundaram
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D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004049:Diethylcarbamazine / Q000032:analysis* / Q000592:standards / Q000627:therapeutic use; D004605:Elephantiasis, Filarial / Q000188:drug therapy; D005369:Filaricides / Q000032:analysis* / Q000627:therapeutic use; D006801:Humans; D015203:Reproducibility of Results; D017673:Sodium Chloride, Dietary / Q000627:therapeutic use
D005369:Filaricides; D017673:Sodium Chloride, Dietary; D004049:Diethylcarbamazine