Adhesion via CD43 induces Syk activation and cell proliferation in TF-1 cells.
The effect of adhesion via CD43 (leukosialin, sialophorin) on cell proliferation and phosphorylation signaling were examined in a growth factor-dependent hematopoietic progenitor cell line, TF-1. TF-1 cells promptly resulted in death after withdrawal of growth factors. However, the viable cell number increased when TF-1 cells were cultured on anti-CD43 monoclonal antibody-coated plates. In this case, sustained activation of protein tyrosine kinase Syk and extracellular signal-regulated kinase (Erk) 1/2 were detected. Overexpression of exogenous Syk on TF-1 cells by the adenovirus vector system induced enhancement of the cell proliferation accompanied with enhancement of the Erk activation by a dominant-positive effect. The signal transducer and activator of transcription (STAT) 5 seemed not to be associated with the CD43-mediated cell proliferation. These results indicated that adhesion via CD43 induces the proliferation of TF-1 cells in the absence of growth factors in part by Syk-dependent Erk 1/2 signaling.
Miura
Y
Y
Department of Hematology and Oncology, Graduate School of Medicine, Kyoto University, Sakyo-ku, Kyoto 606-8507, Japan.
Mizutani
C
C
Nishihara
T
T
Hishita
T
T
Yanagi
S
S
Tohyama
Y
Y
Ichiyama
S
S
Yamamura
H
H
Uchiyama
T
T
Tohyama
K
K
D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D015703:Antigens, CD; D002448:Cell Adhesion; D002455:Cell Division; D002460:Cell Line; D004789:Enzyme Activation; D004792:Enzyme Precursors / Q000235:genetics / Q000378:metabolism*; D005822:Genetic Vectors; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D047908:Intracellular Signaling Peptides and Proteins; D007700:Kinetics; D051917:Leukosialin; D020935:MAP Kinase Signaling System; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism; D048052:Mitogen-Activated Protein Kinase 3; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D010766:Phosphorylation; D019000:Phosphotyrosine / Q000378:metabolism; D011505:Protein-Tyrosine Kinases / Q000235:genetics / Q000378:metabolism*; D012795:Sialoglycoproteins / Q000502:physiology*; D000072377:Syk Kinase; D014162:Transfection
D015703:Antigens, CD; D004792:Enzyme Precursors; D047908:Intracellular Signaling Peptides and Proteins; D051917:Leukosialin; D012795:Sialoglycoproteins; D019000:Phosphotyrosine; D011505:Protein-Tyrosine Kinases; D000072377:Syk Kinase; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; D020928:Mitogen-Activated Protein Kinases