RecA protein filaments disassemble in the 5' to 3' direction on single-stranded DNA.
RecA protein forms filaments on both single- and double-stranded DNA. Several studies confirm that filament extension occurs in the 5' to 3' direction on single-stranded DNA. These filaments also disassemble in an end-dependent fashion, and several indirect observations suggest that the disassembly occurs on the end opposite to that at which assembly occurs. By labeling the 5' end of single-stranded DNA with a segment of duplex DNA, we demonstrate unambiguously that RecA filaments disassemble uniquely in the 5' to 3' direction.
Bork
J M
JM
Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706-1544, USA.
Cox
M M
MM
Inman
R B
RB
D004277:DNA, Single-Stranded / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D008854:Microscopy, Electron; D011938:Rec A Recombinases / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure
D004277:DNA, Single-Stranded; D004268:DNA-Binding Proteins; D011938:Rec A Recombinases