Induction of tumor necrosis factor-alpha by cefodizime in U-937 cells.
Cefodizime has modulating effects on the release of diverse cytokines. We determined the modulator activity of this antibiotic on the production of TNF in human monocytic U-937 cells. The measurement of TNF was carried out by ELISA test and by a L-929 cells-based citotoxic bioassay. The results showed that cefodizime alone induced the production of TNF on U-937 cells, however, the addition of LPS led to a decrease in the release of this cytokine (p < 0.05). On the other hand, the combination of cefodizime-PMA had a synergic effect (p < 0.05), while addition of LPS to this combination caused a decrease of TNF production (p < 0.05). With these results we conclude that cefodizime modulates the production of TNF in U-937 cells, which is down regulated by the addition of LPS.
Guerra-Infante
F M
FM
fguerrai@mailer.main.conacyt.mx
Zamora-Ruíz
A
A
López-Hurtado
M
M
Flores-Medina
S
S
D002439:Cefotaxime / Q000031:analogs & derivatives / Q000494:pharmacology*; D002460:Cell Line; D002511:Cephalosporins / Q000494:pharmacology*; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology; D009000:Monocytes / Q000187:drug effects*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
D002511:Cephalosporins; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D002439:Cefotaxime; D013755:Tetradecanoylphorbol Acetate; C033356:cefodizime