title: Estrogen affects the expression of Ca2+/calmodulin-dependent protein kinase IV in amygdala.
abstract: We examined the effects of long-term estradiol benzoate (E2) treatment on protein expression of Ca2+/calmodulin-dependent protein kinase IV (CaMK IV) in the amygdala of ovariectomized (OVX) rats. Western blot analysis revealed increased protein levels of CaMK IV in the nuclear but not in the membranal or cystolic fraction of total amygdala in E2-treated compared to OVX rats. Significant increases in levels of CaM kinase IV gold immunolabeling were seen in the medial and basomedial, but not in the central or basolateral, amygdala of E2 compared to OVX rats, indicating the neuroanatomical heterogeneity of the E2 effect. These results suggest that CaMK IV may act as a molecular target for actions of estrogen in the amygdala of rats.
authors.affiliation: Department of Anatomy and Cell Biology, University of Illinois at Chicago, 808 South Wood Street (M/C 512), Chicago, IL 60612, USA.
authors.forename: J
authors.initials: J
authors.lastname: Zhou
authors.forename: R S
authors.initials: RS
authors.lastname: Cohen
authors.forename: S C
authors.initials: SC
authors.lastname: Pandey
mesh_terms: D000199:Actins / Q000187:drug effects / Q000378:metabolism; D000679:Amygdala / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology*; D000818:Animals; D020013:Calcium Signaling / Q000187:drug effects* / Q000502:physiology; D054734:Calcium-Calmodulin-Dependent Protein Kinase Type 4; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000187:drug effects* / Q000378:metabolism; D002451:Cell Compartmentation / Q000187:drug effects / Q000502:physiology; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D003071:Cognition / Q000187:drug effects / Q000502:physiology; D017362:Cyclic AMP Response Element-Binding Protein / Q000187:drug effects* / Q000378:metabolism; D004334:Drug Administration Schedule; D004644:Emotions / Q000187:drug effects / Q000502:physiology; D004967:Estrogens / Q000378:metabolism / Q000494:pharmacology*; D005260:Female; D007150:Immunohistochemistry; D009474:Neurons / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D010052:Ovariectomy; D010766:Phosphorylation / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D013347:Subcellular Fractions / Q000187:drug effects / Q000378:metabolism
chemical_list: D000199:Actins; D017362:Cyclic AMP Response Element-Binding Protein; D004967:Estrogens; D054734:Calcium-Calmodulin-Dependent Protein Kinase Type 4; D017871:Calcium-Calmodulin-Dependent Protein Kinases; C517686:Camk4 protein, rat