title: N-terminal acetylation in a third protein family of vertebrate alcohol dehydrogenase/retinal reductase found through a 'proteomics' approach in enzyme characterization.
abstract: A recent finding of a novel class of retinol-active alcohol dehydrogenase (ADH) in frog prompted analysis of this activity in other vertebrate forms. Surprisingly, yet another and still more unrelated ADH was identified in chicken tissues. It was found to be a member of the aldo-keto reductase (AKR) enzyme family, not previously known as an ADH in vertebrates. Its terminal blocking group and the N-terminal segment, not assigned by protein and cDNA structure analysis, were determined by electrospray tandem mass spectrometry after protein isolation by two-dimensional gel electrophoresis. The N terminus is Acetyl-Ala- and the N-terminal segment contains two consecutive Asn residues. The results establish the new ADH enzyme of the AKR family and show the usefulness of combined gel separation and mass spectrometry in enzyme-characterization.
authors.affiliation: Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden.
authors.forename: D
authors.initials: D
authors.lastname: Hirschberg
authors.forename: E
authors.initials: E
authors.lastname: Cederlund
authors.forename: B
authors.initials: B
authors.lastname: Crosas
authors.forename: A
authors.initials: A
authors.lastname: Jonsson
authors.forename: S
authors.initials: S
authors.lastname: Tryggvason
authors.forename: J
authors.initials: J
authors.lastname: Farrés
authors.forename: X
authors.initials: X
authors.lastname: Parés
authors.forename: T
authors.initials: T
authors.lastname: Bergman
authors.forename: H
authors.initials: H
authors.lastname: Jörnvall
mesh_terms: D000107:Acetylation; D000426:Alcohol Dehydrogenase / Q000737:chemistry* / Q000235:genetics; D000429:Alcohol Oxidoreductases / Q000737:chemistry* / Q000235:genetics; D000449:Aldehyde Reductase; D000074408:Aldo-Keto Reductases; D000595:Amino Acid Sequence; D000818:Animals; D002645:Chickens; D010446:Peptide Fragments / Q000737:chemistry; D020543:Proteome; D011898:Ranidae; D021241:Spectrometry, Mass, Electrospray Ionization; D014714:Vertebrates
chemical_list: D010446:Peptide Fragments; D020543:Proteome; D000429:Alcohol Oxidoreductases; D000074408:Aldo-Keto Reductases; D000426:Alcohol Dehydrogenase; D000449:Aldehyde Reductase; C084155:alcohol dehydrogenase (NAD(P)+)