A bioassay using the human hepatoblastoma cell line HepG2 for detecting phototoxicity of furocoumarins.
We successfully evaluated the human hepatoblastoma cell line HepG2 as a model to assess phototoxicity of coumarins. Five natural furocoumarins were tested and their phototoxic activities, obtained by measuring cell viability in the presence of UV using the MTT test, were as follows: xanthotoxin (8-MOP) >> heraclenol = trichoclin = imperatorin >> peucedanin, both in growing and confluent cell cultures. This easy-to-perform, miniaturised, quantitative and sensitive method could therefore be used as a primary screening test for phototoxicity of a large number of compounds and plant extracts.
Colombain
M
M
Laboratoire de Pharmacognosie, Equipe de Chimie Thérapeutique EA482, Faculté de Médecine et de Pharmacie, Place Saint-Jacques, Besançon Cedex, France.
Goll
V
V
Muyard
F
F
Girard
C
C
Bévalot
F
F
Richert
L
L
D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003374:Coumarins / Q000494:pharmacology* / Q000528:radiation effects; D003603:Cytotoxins / Q000494:pharmacology*; D011564:Furocoumarins / Q000494:pharmacology* / Q000528:radiation effects; D006801:Humans; D010777:Photochemistry; D010936:Plant Extracts / Q000494:pharmacology* / Q000528:radiation effects; D027821:Rutaceae; D012680:Sensitivity and Specificity; D018675:Toxicity Tests / Q000379:methods*; D014407:Tumor Cells, Cultured; D014466:Ultraviolet Rays
D003374:Coumarins; D003603:Cytotoxins; D011564:Furocoumarins; D010936:Plant Extracts