Identification of Staphylococcus spp. by PCR-restriction fragment length polymorphism of gap gene.
Oligonucleotide primers specific for the Staphylococcus aureus gap gene were previously designed to identify 12 Staphylococcus spp. by PCR. In the present study, AluI digestion of PCR-generated products rendered distinctive restriction fragment length polymorphism patterns that allowed 24 Staphylococcus spp. to be identified with high specificity.
Yugueros
J
J
Departamento de Sanidad Animal, Microbiología e Inmunología, Universidad de León, 24071 León, Spain.
Temprano
A
A
Sánchez
M
M
Luengo
J M
JM
Naharro
G
G
D000818:Animals; D004269:DNA, Bacterial / Q000032:analysis; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D013045:Species Specificity; D013211:Staphylococcus aureus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification
D004269:DNA, Bacterial; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; C024669:endodeoxyribonuclease AluI; D015252:Deoxyribonucleases, Type II Site-Specific