title: Gene transfer by adenovirus in rabbit iris sphincter muscle.
abstract: The effect of insertion of an exogenous gene on smooth muscle function in rabbit iris sphincter muscle was investigated. An adenoviral vector encoding the bacterial LacZ gene (AdLacZ, 10(7) pfu) and viscoelastics were injected into the posterior chamber of eyes of albino rabbits. Three days after injection, the effects of acetylcholine (Ach), carbachol (Carb), substance P (SP) and electrical field stimuli on isolated iris sphincter were investigated using isometric tension-recording methods. X-Gal histostaining showed that iris sphincter smooth muscle cells were transfected in 7 of 11 muscle strips. Contraction-response curves for Ach, Carb or SP were not different from control. We conclude that the iris sphincter muscle can be gene-transfected by posterior chamber infusion of an adenoviral vector with viscoelastics. Adenovirus-mediated gene transfer per se had no measurable effect on tension development.
authors.affiliation: Department of Ophthalmology, Wakayama Medical College, Wakayama, Japan. yoshitom@wakayama-med.ac.jp
authors.forename: T
authors.initials: T
authors.lastname: Yoshitomi
authors.forename: T
authors.initials: T
authors.lastname: Sakamoto
authors.forename: Y
authors.initials: Y
authors.lastname: Ohnishi
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D002217:Carbachol / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004558:Electric Stimulation; D005697:Galactosides; D015870:Gene Expression; D007211:Indoles; D007498:Iris / Q000187:drug effects / Q000378:metabolism* / Q000821:virology; D007763:Lac Operon / Q000502:physiology*; D008297:Male; D009119:Muscle Contraction / Q000502:physiology; D009130:Muscle, Smooth / Q000187:drug effects / Q000378:metabolism* / Q000821:virology; D011817:Rabbits; D013373:Substance P / Q000494:pharmacology; D014162:Transfection; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D005697:Galactosides; D007211:Indoles; D013373:Substance P; D002217:Carbachol; D001616:beta-Galactosidase; D000109:Acetylcholine; C044888:5-bromo-4-chloro-3-indolyl beta-galactoside