title: Acute regulation of glucose transport in a human megakaryocytic cell line: difference between growth factors and H(2)O(2).
abstract: The present study was undertaken to: (i) compare the effect of some hematopoietic growth factors, like interleukine-3, thrombopoietin, granulocyte-megakaryocyte colony-stimulating factor, stem cell factor, and reactive oxygen species such as H(2)O(2) on glucose uptake in a human leukemic megakaryocytic cell line, M07; (ii) investigate the changes in kinetic parameters of the transport activity induced by these stimuli; and (iii) evaluate the effect of genistein, a tyrosine kinase inhibitor, on the glucose uptake activation by the cited agents. The results are as follows: (i) exposure of M07 cells to thrombopoietin, granulocyte-megakaryocyte colony-stimulating factor, and stem cell factor resulted in a rapid stimulation of glucose transport; interleukine-3-treated cells exhibited no increase in the rate of glucose uptake, although M07 proliferation is interleukine-3 dependent; a rapid glucose transport enhancement was also observed when M07 cells were exposed to low doses of H(2)O(2); (ii) the transport kinetic parameters point out that an important difference exists between the effect of cytokines and that of H(2)O(2): cytokines increased predominantly the affinity for glucose, while H(2)O(2) raised both the V(max) and K(m) values; (iii) the isoflavone genistein, at a very low concentration, inhibited the stem cell factor- or H(2)O(2)-induced stimulation of hexose transport, reversing the variations of K(m) and V(max), but it did not affect the transport activity of granulocyte-megakaryocyte colony-stimulating factor-treated cells; and (iv) catalase completely abolished the stimulatory action of H(2)O(2) on glucose transport and slightly prevented the effect of stem cell factor, while caffeic acid phenethyl ester was only able to affect the activation due to stem cell factor.
authors.affiliation: Dipartimento di Biochimica G. Moruzzi, Università di Bologna, Via Irnerio, 48, I-40126 Bologna, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Fiorentini
authors.forename: G
authors.initials: G
authors.lastname: Hakim
authors.forename: L
authors.initials: L
authors.lastname: Bonsi
authors.forename: G P
authors.initials: GP
authors.lastname: Bagnara
authors.forename: T
authors.initials: T
authors.lastname: Maraldi
authors.forename: L
authors.initials: L
authors.lastname: Landi
mesh_terms: D002109:Caffeic Acids / Q000494:pharmacology; D002374:Catalase / Q000378:metabolism / Q000494:pharmacology; D003847:Deoxyglucose / Q000819:agonists / Q000493:pharmacokinetics; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D019833:Genistein / Q000494:pharmacology*; D005947:Glucose / Q000819:agonists* / Q000493:pharmacokinetics*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000378:metabolism / Q000494:pharmacology; D006133:Growth Substances / Q000378:metabolism* / Q000494:pharmacology; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism* / Q000494:pharmacology; D007377:Interleukin-3 / Q000378:metabolism / Q000494:pharmacology; D007700:Kinetics; D007947:Leukemia, Megakaryoblastic, Acute / Q000378:metabolism; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives* / Q000494:pharmacology; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism; D019089:Stem Cell Factor / Q000378:metabolism / Q000494:pharmacology; D013926:Thrombopoietin / Q000378:metabolism / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000378:metabolism
chemical_list: D002109:Caffeic Acids; D004791:Enzyme Inhibitors; D006133:Growth Substances; D007377:Interleukin-3; D019089:Stem Cell Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D013926:Thrombopoietin; D003847:Deoxyglucose; D006861:Hydrogen Peroxide; D019833:Genistein; D002374:Catalase; D011505:Protein-Tyrosine Kinases; C055494:caffeic acid phenethyl ester; D005947:Glucose; D010626:Phenylethyl Alcohol