Unique temperature-sensitive defect in vaccinia virus morphogenesis maps to a single nucleotide substitution in the A30L gene.
Marker rescue experiments demonstrated that the genetic lesion of a previously isolated vaccinia virus temperature-sensitive mutant which forms multilayered envelope structures with lucent interiors and foci of viroplasm with dense centers mapped to the A30L open reading frame. A single base change, resulting in a nonconservative Ser-to-Phe substitution at residue 17, was associated with degradation of the A30L protein at elevated temperatures.
Szajner
P
P
Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892-0445, USA.
Weisberg
A S
AS
Moss
B
B
D001483:Base Sequence; D005814:Genes, Viral; D008969:Molecular Sequence Data; D009024:Morphogenesis; D009154:Mutation; D016366:Open Reading Frames; D013696:Temperature; D014616:Vaccinia virus / Q000235:genetics* / Q000254:growth & development / Q000648:ultrastructure