Indication of transposition of a mobile DNA element containing the vat(D) and erm(B) genes in Enterococcus faecium.
The vat(D) and erm(B) genes encoding streptogramin resistance in Enterococcus faecium transferred together, and a direct physical link between erm(B) and vat(D) was detected. Both the vat(D) and erm(B) probes hybridized to fragments of different sizes in the donor and transconjugants, which indicated a transposition event.
Hammerum
A M
AM
Danish Veterinary Laboratory, Bülowsvej 27, DK-1790 Copenhagen V, Denmark. ama@ssi.dk
Flannagan
S E
SE
Clewell
D B
DB
Jensen
L B
LB
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D015139:Blotting, Southern; D003227:Conjugation, Genetic; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics*; D004351:Drug Resistance; D016984:Enterococcus faecium / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics*; D008780:Methyltransferases / Q000235:genetics*; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction; D025361:Streptogramins / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D025361:Streptogramins; D008780:Methyltransferases; C035181:rRNA (adenosine-O-2'-)methyltransferase