[The effect of thrombin on tissue factor activity in cultured bovine aortic endothelial cells and its correlation with PKC pathway].
OBJECTIVE
To study the stimulating effect of thrombin on the expression of tissue factor (TF) activity in cultured bovine aortic endothelial cells (BAEC) and to elucidate if it is associated with the PKC system.


METHODS
The BAECs were cultured after the Schwartz's method and one stage clotting time assay was used to measure TF activity in BAEC lysates. The TF activity was identified by EDGR-VIIa and a monoclonal antibody against TF.


RESULTS
Thrombin enhanced the expression of TF activity in cultured BAEC and the effect was dose- (r = 0.78, P < 0.05, n = 8) and time-dependent (r = 0.88, P < 0.05, n = 8). PMA, a PKC activator, also induced the expression of TF activity in BAEC. H7, a protein kinase C inhibitor, inhibited the inductive effects of PMA and thrombin on TF in BAEC.


CONCLUSIONS
Thrombin can induce the expression of TF activity in cultured BAEC and the effect depends on PKC pathway.
Li
Z
Z
Department of Physiology, Hunan Medical University, ChangSha 410078.
Wen
Z
Z
Han
J
J
D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine / Q000494:pharmacology; D000818:Animals; D001011:Aorta / Q000166:cytology; D002417:Cattle; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology* / Q000378:metabolism; D011493:Protein Kinase C / Q000037:antagonists & inhibitors; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013917:Thrombin / Q000494:pharmacology*; D013925:Thromboplastin / Q000378:metabolism*
D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D013925:Thromboplastin; D011493:Protein Kinase C; D013917:Thrombin; D013755:Tetradecanoylphorbol Acetate