title: Fusion of C3d molecule with bovine rotavirus VP7 or bovine herpesvirus type 1 glycoprotein D inhibits immune responses following DNA immunization.
abstract: The binding of the complement C3d molecule with receptors on B cells and/or follicular dendritic cells (FDCs) influences the induction of humoral immune responses. For example, C3d fused to an antigen has been shown to have a strong adjuvant effect on antibody production. We investigated the possibility that co-expression of antigen and C3d as a fusion protein could enhance antigen-specific immune responses, following plasmid immunization. One or two copies of murine C3d-cDNA, C3d or (C3d)(2), respectively, were cloned together with bovine rotavirus (BRV) VP7 or bovine herpesvirus type 1 (BHV-1) glycoprotein D (gD) genes. All constructs contained a signal peptide that resulted in the secretion of the expressed proteins. In vitro, the characterization of the chimeric proteins indicated that both VP7 and gD retained their antigenicity and the C3d remained biologically active. However, immunization with plasmids encoding VP7-C3d chimeras did not enhance rotavirus-specific antibody responses and the frequency of BRV-specific IFN-gamma secreting cells in the spleens were significantly lower in mice immunized with pVP7-(C3d)(2) when compared with mice immunized with plasmid encoding VP7. The same pattern of immune responses was observed for plasmids encoding gD-C3d. Both gD-specific antibody responses and the frequency of gD-specific IFN-gamma secreting cells were significantly lower in mice immunized with plasmid expressing gD-C3d chimeras when compared with mice immunized with plasmid encoding gD alone. These results indicate that co-expression of C3d with an antigen actually inhibit both humoral and cell-mediated antigen-specific immune responses.
authors.affiliation: Veterinary Infectious Disease Organization (VIDO), University of Saskatchewan, 120 Veterinary Road, Saskatoon, Saskatchewan, Canada S7N 5E3.
authors.forename: S
authors.initials: S
authors.lastname: Suradhat
authors.forename: R P
authors.initials: RP
authors.lastname: Braun
authors.forename: P J
authors.initials: PJ
authors.lastname: Lewis
authors.forename: L A
authors.initials: LA
authors.lastname: Babiuk
authors.forename: S
authors.initials: S
authors.lastname: van Drunen Littel-van den Hurk
authors.forename: P J
authors.initials: PJ
authors.lastname: Griebel
authors.forename: M E
authors.initials: ME
authors.lastname: Baca-Estrada
mesh_terms: D000818:Animals; D000956:Antigens, Viral; D002213:Capsid / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D036022:Capsid Proteins; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology* / Q000517:prevention & control; D015933:Complement C3d / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D016207:Cytokines / Q000032:analysis / Q000096:biosynthesis; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D006566:Herpesviridae Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D007242:Herpesvirus 1, Bovine / Q000737:chemistry / Q000276:immunology*; D007114:Immunization / Q000662:veterinary; D008198:Lymph Nodes / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D012401:Rotavirus / Q000737:chemistry / Q000276:immunology*; D012400:Rotavirus Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D013154:Spleen / Q000276:immunology; D019444:Vaccines, DNA / Q000037:antagonists & inhibitors / Q000276:immunology / Q000592:standards; D014764:Viral Proteins / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D014765:Viral Vaccines / Q000276:immunology
chemical_list: D000956:Antigens, Viral; D036022:Capsid Proteins; D016207:Cytokines; D011993:Recombinant Fusion Proteins; C050779:VP7 protein, Rotavirus; D019444:Vaccines, DNA; D014764:Viral Proteins; D014765:Viral Vaccines; C046998:bovine herpesvirus type-1 glycoproteins; D015933:Complement C3d