title: A new RNA element located in the coding region of a murine endogenous retrovirus can functionally replace the Rev/Rev-responsive element system in human immunodeficiency virus type 1 Gag expression.
abstract: Nuclear export of incompletely spliced RNAs is a prerequisite for retroviral replication. Complex retroviruses like human immunodeficiency virus (HIV) encode a viral transport factor (Rev), which binds to its target sequence on the RNA genome and directs it into the Crm-1-mediated export pathway. Other retroviruses, like Mason-Pfizer monkey virus, contain cis-acting constitutive RNA transport elements (CTE) which achieve nuclear export of intron-containing RNA via cellular transport factors. Here, we describe the identification and characterization of a novel cis-acting orientation-dependent RNA expression element in the coding region of the murine intracisternal A-type particle (IAP) MIA14. This IAP expression element (IAPE) can functionally replace the Rev system in the expression of HIV-1 Gag proteins but functions independently of Crm-1. The presence of this element is needed for the expression of the IAP Gag proteins, indicating its biological significance. The IAPE can be functionally replaced by placing a CTE on the MIA14 RNA, further supporting its role in mRNA export. Northern blot analysis revealed that total RNA, as well as cytoplasmic RNA, was increased when the element was present. The element was mapped to a predicted stem-loop structure in the 3' part of the pol open reading frame. There was no overall homology between the IAPE and the CTE, but there was complete sequence identity between short putative single-stranded loops. Deletion of these loops from the IAPE severely reduced Rev-independent Gag expression.
authors.affiliation: Heinrich-Pette-Institut, D-20251 Hamburg, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Wodrich
authors.forename: J
authors.initials: J
authors.lastname: Bohne
authors.forename: E
authors.initials: E
authors.lastname: Gumz
authors.forename: R
authors.initials: R
authors.lastname: Welker
authors.forename: H G
authors.initials: HG
authors.lastname: Kräusslich
mesh_terms: D000818:Animals; D019556:COS Cells; D020077:Endogenous Retroviruses / Q000235:genetics*; D015683:Gene Products, gag / Q000096:biosynthesis*; D015694:Gene Products, rev / Q000502:physiology*; D007426:Genes, Intracisternal A-Particle / Q000502:physiology*; D015497:HIV-1 / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D012367:RNA, Viral / Q000502:physiology*; D020218:Response Elements; D054321:rev Gene Products, Human Immunodeficiency Virus
chemical_list: D015683:Gene Products, gag; D015694:Gene Products, rev; D012367:RNA, Viral; D054321:rev Gene Products, Human Immunodeficiency Virus