title: Barley telomeres shorten during differentiation but grow in callus culture.
abstract: Eukaryotic chromosomes terminate with long stretches of short, guanine-rich repeats. These repeats are added de novo by a specialized enzyme, telomerase. In humans telomeres shorten during differentiation, presumably due to the absence of telomerase activity in somatic cells. This phenomenon forms the basis for several models of telomere role in cellular senescence. Barley (Hordeum vulgare L.) telomeres consist of thousands of TTTAGGG repeats, closely resembling other higher eukaryotes. In vivo differentiation and aging resulted in reduction of terminal restriction fragment length paralleled by a decrease of telomere repeat number. Dedifferentiation in callus culture resulted in an increase of the terminal restriction fragment length and in the number of telomere repeats. Long-term callus cultures had very long telomeres. Absolute telomere lengths were genotype dependent, but the relative changes due to differentiation, dedifferentiation, and long-term callus culture were consistent among genotypes. A model is presented to describe the potential role of the telomere length in regulation of a cell's mitotic activity and senescence.
authors.affiliation: Department of Crop & Soil Sciences, Washington State University, Pullman WA 99164-6420, USA.
authors.forename: A
authors.initials: A
authors.lastname: Kilian
authors.forename: C
authors.initials: C
authors.lastname: Stiff
authors.forename: A
authors.initials: A
authors.lastname: Kleinhofs