Establishment of a new scirrhous gastric cancer cell line with loss of heterozygosity at E-cadherin locus.
Scirrhous gastric carcinoma, characterized by carcinoma cell proliferation and infiltration with extensive fibrosis in the stroma, frequently causes peritoneal metastasis. We describe here a newly established cell line, OCUM-6, derived from ascites effusion of a scirrhous gastric cancer patient. The cells are floating and round shape, similar to other scirrhous gastric carcinoma cell lines previously reported. Histologic findings of xenografted tumor obtained from OCUM-6 cells showed medullary growth with a poorly differentiated adenocarcinoma containing signet ring cells. LOH at E-cadherin locus 16q22 was observed in the OCUM-6 cells. LOH at E-cadherin locus might be closely associated with histologic findings and metastatic process of scirrhous gastric cancer. The scirrhous gastric cancer cell line, OCUM-6, may be useful for investigation of the mechanisms of peritoneal dissemination and carcinogenesis.
Murahashi
K
K
Department of Surgical Oncology, Osaka City University Medical School, Osaka, Japan.
Yashiro
M
M
Takenaka
C
C
Matsuoka
T
T
Ohira
M
M
Chung
K H
KH
D002293:Adenocarcinoma, Scirrhous / Q000235:genetics / Q000473:pathology*; D000368:Aged; D000818:Animals; D000951:Antigens, Neoplasm; D015820:Cadherins / Q000235:genetics*; D002885:Chromosomes, Human, Pair 16 / Q000235:genetics; D017931:DNA Primers / Q000737:chemistry; D005260:Female; D011905:Genes, ras / Q000235:genetics; D006801:Humans; D019656:Loss of Heterozygosity / Q000235:genetics*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009154:Mutation; D016133:Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000235:genetics / Q000473:pathology*; D014407:Tumor Cells, Cultured
D000951:Antigens, Neoplasm; D015820:Cadherins; D017931:DNA Primers