title: HIV transmissions from a window-period platelet donation.
abstract: Recently, blood centers began investigational testing for HIV RNA by pooled nucleic acid testing (NAT). A 35-year-old frequent platelet donor tested HIV p24 antigen positive, antibody negative before implementation of NAT. He made 2 platelet donations (day -4 and -11) immediately before testing positive for HIV. The donor's HIV seroconversion was monitored, and stored samples were tested retrospectively for HIV RNA. Platelet recipients were tested for HIV infection. The day -4 sample tested positive for HIV RNA by pooled and individual sample NAT. The day -11 sample tested negative for HIV RNA by both NAT tests. The 2 recipients of the day -4 platelets tested HIV RNA and p24 antigen positive. The recipient of the day -11 platelets could not be tested because he had died. HIV NAT would have prevented transmission of HIV had it been available at the time of this donor's HIV seroconversion.
authors.affiliation: Sacramento Medical Foundation Blood Centers, 1625 Stockton Blvd, Sacramento, CA 95186-7089, USA.
authors.forename: P M
authors.initials: PM
authors.lastname: Kopko
authors.forename: L P
authors.initials: LP
authors.lastname: Fernando
authors.forename: E N
authors.initials: EN
authors.lastname: Bonney
authors.forename: J L
authors.initials: JL
authors.lastname: Freeman
authors.forename: P V
authors.initials: PV
authors.lastname: Holland
mesh_terms: D000328:Adult; D000368:Aged; D000914:Antibodies, Viral / Q000097:blood; D001782:Blood Donors; D015153:Blotting, Western; D006678:HIV / Q000235:genetics; D016655:HIV Core Protein p24 / Q000097:blood; D015658:HIV Infections / Q000635:transmission*; D006679:HIV Seropositivity; D015497:HIV-1 / Q000235:genetics / Q000276:immunology; D015498:HIV-2 / Q000276:immunology; D006801:Humans; D008297:Male; D008875:Middle Aged; D017713:Platelet Transfusion; D010983:Plateletpheresis; D012367:RNA, Viral / Q000097:blood; D013997:Time Factors
chemical_list: D000914:Antibodies, Viral; D016655:HIV Core Protein p24; D012367:RNA, Viral