title: [Isolation and some properties of a lectin from the venom of the Vietnamese scorpion Buthus occitanus sp].
abstract: A lectin was isolated from the venom of scorpion Buthus occitanus sp. by means of Sephadex G-50 gel filtration and CM-cellulose ion exchange chromatography. The homogeneous lectin preparation consisted of homodimeric molecules with a subunit Mr of 9.3 kDa. Glycine, alanine, and serine dominated in the lectin amino acid composition. The lectin was a glycoprotein containing 20% carbohydrates (predominantly mannose and glucose). Trypsin-treated murine erythrocytes agglutinated at the lectin concentration of 32 micrograms/ml. Hemagglutination was inhibited by carbohydrates (L-fucose > D-glucose > L-rhamnose > D-xylose). The lectin revealed no phospholipase or hyaluronidase, nor toxic activity.
authors.affiliation: Institute of Organoelemental Compounds, Russian Academy of Sciences, ul. Vavilova 28, Moscow, 11781 Russia.
authors.forename: N A
authors.initials: NA
authors.lastname: Khoang
authors.forename: B B
authors.initials: BB
authors.lastname: Berezin
authors.forename: V M
authors.initials: VM
authors.lastname: Lakhtin
authors.forename: I A
authors.initials: IA
authors.lastname: Iamskov
mesh_terms: D000818:Animals; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D037102:Lectins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D012604:Scorpion Venoms / Q000737:chemistry*; D012605:Scorpions; D013050:Spectrometry, Fluorescence
chemical_list: D037102:Lectins; D012604:Scorpion Venoms
vernacular_title: Vydelenie i nekotorye svoĭstva lektina iz iada V'etnamskogo skorpiona Buthus occitanus sp.