title: [Adenovirus-mediated transfer of human wild-type p53, GM-CSF, and B7-1 genes efficiently produces growth suppression and apoptosis of hepatocellular cells in vitro].
abstract: OBJECTIVE
  To investigate the tumor suppressor activity of recombinant adenovirus vector expressing the human wild-type p53, GM-CSF, and B7-1 proteins (designated as BB-102) in human hepatocellular carcinoma cells (HCC) in vitro.
  
  
  METHODS
  The wild-type p53 BEL-7402, mutant p53 HLE, and HuH-7 HCC cell lines were infected with BB-102 at MOI of 50 in vitro. Immunohistochemical assay was used to determine p53 expressed by BB-102. Tumor suppressor activity of the expressed p53 was identified by terminal deoxynucleotidy I transferase (TdT) assay in BB-102-infected HCC cell lines.
  
  
  RESULTS
  p53 protein was found to express in a dose-dependent manner in BB-102-infected HCC cell lines. The proliferation of HCC cell lines were suppressed significantly at the average rates of 58.5%, 81.5%, and 71.1% for BEL-7402, HLE, and HuH-7 respectively from 4 to 10 days, accompanying inducing apoptosis in BB-102-infected HCC cell lines.
  
  
  CONCLUSIONS
  Besides the expression of B7-1 and GM-CSF, BB-102 is able to express p53 protein in independent manner and exerts its anti-tumor activity, which suggests that BB-102 may be useful for gene therapy against HCC in vivo.
authors.affiliation: Division of Biological Engineering, Institute of Infectious Disease, the 302nd Hospital of PLA, Beijing 100039, China.
authors.forename: M
authors.initials: M
authors.lastname: Shi
authors.forename: F
authors.initials: F
authors.lastname: Wang
authors.forename: M
authors.initials: M
authors.lastname: Liu
authors.forename: B
authors.initials: B
authors.lastname: Zhang
authors.forename: Z
authors.initials: Z
authors.lastname: Qiu
authors.forename: Z
authors.initials: Z
authors.lastname: Lei
authors.forename: N
authors.initials: N
authors.lastname: Masayoshi
authors.forename: L
authors.initials: L
authors.lastname: Gao
authors.forename: Z
authors.initials: Z
authors.lastname: Wu
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D017209:Apoptosis; D018122:B7-1 Antigen / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D006528:Carcinoma, Hepatocellular / Q000473:pathology; D002455:Cell Division / Q000187:drug effects; D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D005822:Genetic Vectors; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology; D014407:Tumor Cells, Cultured; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D018122:B7-1 Antigen; D016159:Tumor Suppressor Protein p53; D016178:Granulocyte-Macrophage Colony-Stimulating Factor