[Immune activation genes expression in CD28-costimulated lymphocytes and its application as a diagnostic tool in kidney transplantation].
OBJECTIVE
To investigate the detection of immune activation genes expression as an indicator for the diagnosis of immunological rejection in renal transplantation.


METHODS
In 61 renal allograft recipients, the immune activation genes expression of CD28-costimulated peripheral blood mononuclear cells (PBMC) was studied by RT-PCR.


RESULTS
The mRNA expression positive rate of IL-10, CD40L, Fas, Perforin, IL-2, IFN-gamma, FasL, bcl-xL, Granzyme B (GB), TGF-beta 1 and IL-4 was 91.8%, 70.5%, 88.5%, 96.7%, 41%, 54.1%, 60.7%, 67.2%, 67.2%, 54.1% and 32.8% respectively. IL-2, IFN-gamma, FasL and Granzyme B were screened out as indicators for the diagnosis of allograft rejection. Any three or more of IL-2, IFN-gamma, FasL, and Granzyme B genes expression could be used as a diagnostic tool for rejection with much favorable sensitivity (93%) and specificity (75%).


CONCLUSIONS
The detection of IL-2, IFN-gamma, FasL and Granzyme B genes expression is beneficial for the evaluation of immunological state in renal allograft recipients. This non-invasive method can be used for clinical diagnosis of allograft rejection.
Zhou
P
P
Center of Renal Transplantation, Shanghai First People's Hospital, Shanghai 200080.
Wang
X
X
Tang
X
X
D000328:Adult; D018106:CD28 Antigens / Q000276:immunology; D005260:Female; D015870:Gene Expression; D006084:Graft Rejection / Q000276:immunology*; D053804:Granzymes; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis* / Q000235:genetics; D007376:Interleukin-2 / Q000096:biosynthesis* / Q000235:genetics; D016030:Kidney Transplantation / Q000276:immunology*; D008297:Male; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D012697:Serine Endopeptidases / Q000096:biosynthesis* / Q000235:genetics
D018106:CD28 Antigens; D007376:Interleukin-2; D012333:RNA, Messenger; D007371:Interferon-gamma; C509213:GZMB protein, human; D053804:Granzymes; D012697:Serine Endopeptidases