title: [Determination of content of radix Paeoniae Rubar(Chishao)801 in rabbit's plasma by HPLC].
abstract: A HPLC method was used to determine the content of Radix Paeoniae Rubar (Chishao)801 in rabbit's plasma, using Nucleosil C18 column, methanol, water and glacial acetic acid (48:50:2) as mobile phase ethye 4-hydroxybenzoate as internal standard, and UV-detector(lambda max: 275 nm, AUFS: 0.02). The average recovery was 96.70%(C: 2.5 micrograms/ml) and 98.86%(C: 5 micrograms/ml) respectively. The lowest determination limit was 0.1 microgram/ml. The method is simple, accurate, sensitive and reliable.
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Traditional Chinese Medicine, Beijing 100700.
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.forename: J
authors.initials: J
authors.lastname: Li
authors.forename: G
authors.initials: G
authors.lastname: Du
authors.forename: X
authors.initials: X
authors.lastname: Wang
authors.forename: H
authors.initials: H
authors.lastname: Cui
authors.forename: Y
authors.initials: Y
authors.lastname: Zhao
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D008297:Male; D011435:Propyl Gallate / Q000097:blood*; D011817:Rabbits
chemical_list: D004365:Drugs, Chinese Herbal; D011435:Propyl Gallate