[Analysis of complex formation of daunomycin with the deoxyhexanucleotide 5'-d(TpApCpGpTpA) in an aqueous solution from NMR-spectroscopy data].
Self-association of hexadeoxynucleotide 5'-d(TpApCpGpTpA) and its complexation with antitumor antibiotic daunomycin were studied by one- and two-dimensional homonuclear 1H NMR spectroscopy and heteronuclear 1H-31P NMR spectroscopy in water-salt solution. The concentration and temperature dependences of proton chemical shifts of the hexadeoxynucleotide and the ligand were measured, and equilibrium constants and thermodynamic parameters of corresponding reactions were calculated on this basis using models for the formation of hexadeoxynucleotide duplex and its complex with the antibiotic. The spatial structure of daunomycin-d(TACGTA)2 complex in solution was calculated using X-PLOR software on the basis of 2D NOE spectral data and the limit values of proton chemical shifts of the ligand. Comparative analysis of different intermolecular interactions in sequence-specific binding of the antibiotic to the DNA fragment was carried out.
Veselkov
A N
AN
veselkov@sevgtu.sebastopol.ua
Eaton
R J
RJ
Pakhomov
V I
VI
Semanin
A V
AV
Baranovskii
S F
SF
Dymant
L N
LN
Davies
D B
DB
D003630:Daunorubicin / Q000737:chemistry*; D003854:Deoxyribonucleotides / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D012996:Solutions; D013696:Temperature; D014867:Water
D003854:Deoxyribonucleotides; D012996:Solutions; D014867:Water; D003630:Daunorubicin
Analiz kompleksoobrazovaniia daunomitsina s dezoksigeksanukleotidom 5'-d(TpApCpGpTpA) v vodom rastvore po dannym IaMR-spektroskopii.