Effect of burn healing liquid on keratinocyte and fibroblast proliferation and on collagen lattice contraction.
OBJECTIVE
To investigate the effect of Burn Healing Liquid (BHL) on the proliferation of keratinocytes and fibroblasts and to explore the potential effect of BHL on fibroblast contraction.


METHODS
Human keratinocytes and dermal fibroblasts were cultured in media containing serial dilutions of BHL followed by cell proliferation determination assessed with MTT (3-[4, 5-dimehtylthiazol-2-yl]-2, 5-diphenyl tetrazolium bromide) assay at different time intervals. The in vitro collagen lattice contraction model was utilized for determining the contractility of fibroblasts cultured in BHL containing medium.


RESULTS
The 1:10(7) dilution of BHL enhanced the growth of both keratinocytes and fibroblasts whereas the 1:10 dilution increased the growth of keratinocytes only. Collagen lattice contraction was inhibited dose-dependently by BHL and such an inhibition could be reversed by switching BHL containing medium to normal medium containing 10% fetal calf serum.


CONCLUSIONS
BHL enhances the growth of both keratinocytes and fibroblasts and reversibly inhibits the fibroblast contraction in collagen lattice.
Su
Y
Y
Department of Burn Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China. burns@fmmu.edu.cn
Chen
B
B
Thang
P T
PT
Hughes
M A
MA
Xu
X
X
Cherry
G W
GW
D002056:Burns / Q000150:complications; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002921:Cicatrix / Q000517:prevention & control; D003094:Collagen / Q000187:drug effects*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects; D015603:Keratinocytes / Q000166:cytology* / Q000187:drug effects; D012867:Skin / Q000166:cytology
D004365:Drugs, Chinese Herbal; D003094:Collagen