NG2 immunoreactivity on human brain endothelial cells.
In this study, we evaluated the expression of NG2 on human brain endothelial cells derived from temporal lobe tissue resected as a treatment for intractable epilepsy. Using dissociated cell cultures, we found expression of NG2 on both proliferating and non-proliferating cells, at the mRNA level by reverse transcription-polymerase chain reaction analyses, and at the protein level by immunocytochemistry and immunoblotting. We further observed that human cerebral microvessels in nonmalignant CNS tissues immunoreacted with NG2. NG2 protein was detected using both a rabbit antibody raised against the rodent NG2 and a monoclonal antibody raised against the human NG2 (9.2.27). Our findings further define the range of resident cells of the CNS that can express NG2 and indicate that expression of NG2 by endothelial cells is not restricted to proliferating CNS endothelial cells or to endothelial cells found in brain tumors.
Pouly
S
S
Neuroimmunology Unit, Montreal Neurological Institute, Quebec, Canada.
Prat
A
A
Blain
M
M
Olivier
A
A
Antel
J
J
D000328:Adult; D000911:Antibodies, Monoclonal; D000941:Antigens / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D001932:Brain Neoplasms / Q000503:physiopathology; D004730:Endothelium, Vascular / Q000737:chemistry* / Q000166:cytology / Q000502:physiology; D015870:Gene Expression / Q000502:physiology; D006801:Humans; D066298:In Vitro Techniques; D008833:Microcirculation / Q000502:physiology; D018919:Neovascularization, Physiologic / Q000502:physiology; D011509:Proteoglycans / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D012333:RNA, Messenger / Q000032:analysis; D013702:Temporal Lobe / Q000098:blood supply* / Q000166:cytology*; D000099216:Chondroitin Sulfate Proteoglycan 4
D000911:Antibodies, Monoclonal; D000941:Antigens; D011509:Proteoglycans; D012333:RNA, Messenger; D000099216:Chondroitin Sulfate Proteoglycan 4