title: Structural analysis of the lipopolysaccharide from the nontypable Haemophilus influenzae strain SB 33.
abstract: The structure of the core region of the lipopolysaccharide (LPS) from the nontypable Haemophilus influenzae strain SB 33 was elucidated. The LPS was subjected to a variety of degradative procedures. The structures of the derived oligosaccharide products were established by monosaccharide and methylation analyses, NMR spectroscopy and mass spectrometry. These analyses revealed a series of related phosphocholine (PCho) containing structures differing in the number of hexose residues. The results pointed to each species containing a conserved phosphoethanolamine (PEtn) substituted heptose-containing trisaccharide inner-core moiety. The major LPS glycoforms were identified as 2-Hex, 3-Hex and 4-Hex species according to the number of hexose residues present.
authors.affiliation: Institute for Biological Sciences, National Research Council, Ottawa, ON, Canada. Andrew.Cox@nrc.ca
authors.forename: A D
authors.initials: AD
authors.lastname: Cox
authors.forename: H
authors.initials: H
authors.lastname: Masoud
authors.forename: P
authors.initials: P
authors.lastname: Thibault
authors.forename: J R
authors.initials: JR
authors.lastname: Brisson
authors.forename: M
authors.initials: M
authors.lastname: van der Zwan
authors.forename: M B
authors.initials: MB
authors.lastname: Perry
authors.forename: J C
authors.initials: JC
authors.lastname: Richards
mesh_terms: D002236:Carbohydrate Conformation; D006193:Haemophilus influenzae / Q000737:chemistry* / Q000145:classification*; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000032:analysis / Q000737:chemistry; D010767:Phosphorylcholine / Q000032:analysis; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D008070:Lipopolysaccharides; D009844:Oligosaccharides; D010767:Phosphorylcholine