title: [Study on electrochemical behavior of HL-60 cells during the etoposide-inducing apoptosis].
abstract: OBJECTIVE
  To study the electrochemical behavior of apoptotic HL-60 cells induced with etoposide.
  
  
  METHODS
  Electrochemical cytosensor was used to monitor HL-60 cells treated with etoposide.
  
  
  RESULTS
  The peak current and electron transfer rate decreased as etoposide dosage increased. The decrease appeared at 2 micrograms/ml etoposide treatment and was remarkable at 20 micrograms/ml, which occurred before the typical changes of cell morphology and DNA contents for apoptosis. FCM-DNA analysis showed that the apoptotic cells were of 10.5%-20% while the peak current decreased from 1.8 microA to 0.4 microA after 2.3 and 4 hours treatment with 200 micrograms/ml etoposide, and the peak current after 4 hours treatment was 5 times lower than that before induction.
  
  
  CONCLUSIONS
  The changes of electrochemical behavior during the early stage of apoptosis occurred earlier and could be accumulated.
authors.affiliation: Institute of Hematology, Beijing Medical University, Beijing 100044.
authors.forename: X
authors.initials: X
authors.lastname: Ren
authors.forename: D
authors.initials: D
authors.lastname: Wang
authors.forename: H
authors.initials: H
authors.lastname: Li
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D004563:Electrochemistry; D005047:Etoposide / Q000494:pharmacology*; D018922:HL-60 Cells / Q000473:pathology; D006801:Humans
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D005047:Etoposide