UV-irradiation-induced DNA immobilization and functional utilization of DNA on nonwoven cellulose fabric.
Immobilization of double-stranded DNA onto nonwoven cellulose fabric by UV irradiation and utilization of DNA-immobilized cloth were examined. The immobilized DNA was found to be stable in water, with the maximum amount of fabric-immobilized DNA being approximately 20 mg/g of nonwoven fabric. The DNA-immobilized cloth could effectively accumulate endocrine disruptors and harmful DNA intercalating pollutants, such as dibenzo-p-dioxin, dibenzofuran, biphenyl, benzo[a]pyrene and ethidium bromide. Additionally, DNA-immobilized cloth was found to bind metal ions, such as Ag+, Cu2+, and Zn2+. The maximum amounts of bound Ag+, Cu2+, and Zn2+ onto DNA-immobilized cloth (1 g) were approximately 5, 2, and 1 mg, respectively. DNA-immobilized cloth containing Ag+ showed antibacterial activity against Escherichia coli and Staphylococcus aureus. DNA-immobilized cloth without metal ion and with Cu2+ or Zn2+ did not show antibacterial activity. These results suggest that immobilized DNA imparts useful functionality to cloth. DNA-immobilized cloth prepared by UV irradiation has potential to serve as a useful biomaterial for medical, engineering, and environmental application.
Yamada
M
M
Laboratory of Bio-Material Chemistry, Division of Bioscience, Graduate School of Environmental Earth Science, Hokkaido University, Sapporo, Japan.
Kato
K
K
Shindo
K
K
Nomizu
M
M
Haruki
M
M
Sakairi
N
N
Ohkawa
K
K
Yamamoto
H
H
Nishi
N
N
D002482:Cellulose; D004247:DNA; D004702:Endocrine Glands / Q000187:drug effects; D004926:Escherichia coli / Q000254:growth & development; D011078:Polychlorinated Biphenyls / Q000494:pharmacology; D013211:Staphylococcus aureus / Q000254:growth & development; D014466:Ultraviolet Rays
D002482:Cellulose; D004247:DNA; D011078:Polychlorinated Biphenyls