title: Increased alpha3-fucosylation of alpha1-acid glycoprotein in Type I diabetic patients is related to vascular function.
abstract: Diabetic mellitus is attended by the development of endothelial dysfunction which is suggested to be accompanied with a chronic low-degree of inflammation. During a chronic hepatic inflammatory response, specific changes in glycosylation of the acute phase protein alpha1-acid glycoprotein (AGP) can be detected. In this report we studied the changes in glycosylation of AGP in more detail and evaluated the relation between a change in glycosylation of AGP and urinary albumin secretion in Type I diabetic patients. The glycosylation of AGP, studied by crossed affinity immunoelectrophoresis (CAIE) and high pH anion exchange chromatography with pulse amperometric detection (HPAEC-PAD), showed an increase in alpha3-fucosylation. Staining with an antibody against sialyl Lewis(x) (sLe(x)) implied that part of the alpha3-fucosylation was present in a sLe(x)-conformation. In the group of Type I diabetic patients with increased urinary albumin excretion, a significant increase in alpha3-fucosylation of AGP (p<0.0005) could be detected. Therefore, the increased alpha3-fucosylation of AGP can be used as an additional marker for the development of vascular complications in Type I diabetic patients.
authors.affiliation: Department of Molecular Cell Biology, Glycoimmunology Group, Institute for Immunology and Inflammatory diseases, Faculty of Medicine, VU Medical Center, Amsterdam, The Netherlands.
authors.forename: D C
authors.initials: DC
authors.lastname: Poland
authors.forename: C G
authors.initials: CG
authors.lastname: Schalkwijk
authors.forename: C D
authors.initials: CD
authors.lastname: Stehouwer
authors.forename: C A
authors.initials: CA
authors.lastname: Koeleman
authors.forename: B
authors.initials: B
authors.lastname: van het Hof
authors.forename: W
authors.initials: W
authors.lastname: van Dijk
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000419:Albuminuria; D002236:Carbohydrate Conformation; D002852:Chromatography, Ion Exchange / Q000379:methods; D003208:Concanavalin A / Q000378:metabolism; D003922:Diabetes Mellitus, Type 1 / Q000378:metabolism*; D003925:Diabetic Angiopathies / Q000378:metabolism*; D005260:Female; D005643:Fucose / Q000378:metabolism; D005647:Fucosyltransferases / Q000097:blood; D006031:Glycosylation; D006801:Humans; D007249:Inflammation; D037102:Lectins / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009844:Oligosaccharides / Q000378:metabolism; D009961:Orosomucoid / Q000737:chemistry* / Q000378:metabolism*; D000080506:Sialyl Lewis X Antigen; D013223:Statistics as Topic
chemical_list: D037102:Lectins; D009844:Oligosaccharides; D009961:Orosomucoid; D000080506:Sialyl Lewis X Antigen; C064549:lectin, Aleuria aurantia; D003208:Concanavalin A; D005643:Fucose; D005647:Fucosyltransferases