[Subforms of transcription factor Oct-1 synthesized in lymphocytes].
Transcription factor Oct-1 is ubiquitous, participating in expression of the cell housekeeping genes as well as in differentiation of lymphocytes and activation of transcription of immunoglobulin genes in B cells. A new tissue-specific form of Oct-1 (Oct-1L) was found in lymphoid cells of bone marrow, lymph nodes, spleen, thymus, as well as in the cell lines of B and T lymphocytes at different stages of differentiation. This isoform was not found in embryonal and nonlymphoid tissues and cell lines. The complete structure of the oct-1L mRNA was determined, which generally corresponds to the structure of mouse Oct-1b. The difference between oct-1L and isoforms oct-a, b, and c functioning in all cells is replacement of the long first 5'-terminal exon (the 1U exon) encoding 21 amino acid residues with a short one encoding 10 amino acids in the isoforms Oct-1L and Oct-1R. Interestingly, attempts to find mature oct-1 mRNA simultaneously containing the 1L and 1U exons were unsuccessful. The parallel synthesis of "ubiquitous" isoforms Oct-1a, b, and c as well as tissue-specific Oct-1L and Oct-1R in lymphocytes and their precursors may be due either to a high demand of these cells for Oct-1 or to selective participation of different Oct-1 isoforms in regulation of the housekeeping genes and genes involved in the B and T cell differentiation and synthesis of imminoglobulins.
Pankratova
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EV
Deev
I E
IE
Zhenilo
S V
SV
Anfalova
T V
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Polianovskiĭ
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OL
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary; D004268:DNA-Binding Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D051863:Host Cell Factor C1; D008214:Lymphocytes / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D050811:Octamer Transcription Factor-1; D020033:Protein Isoforms / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics
D017931:DNA Primers; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; C497693:Hcfc1 protein, mouse; D051863:Host Cell Factor C1; D050811:Octamer Transcription Factor-1; C494166:Pou2f1 protein, mouse; D020033:Protein Isoforms; D012333:RNA, Messenger; D014157:Transcription Factors
Subformy faktora transkriptsii Oct-1, sinteziruemye v limfotsitakh.