[Relationship of polymorphism of LMP2 gene to insulin-dependent diabetes mellitus and DR3 gene].
OBJECTIVE
To investigate the relationship of polymorphism of large multifunctional protease (LMP) 2 genes to insulin-dependent diabetes mellitus (IDDM) and DR3 gene.


METHODS
The polymorphism of LMP2 genes was identified by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in 68 IDDM patients and 71 healthy controls. IDDM patients and healthy controls were respectively divided into 2 groups according to DR3 genotyping. One group included subjects with DR3 gene, and another group subjects without DR3 gene.


RESULTS
In the subjects with DR3 gene, the frequencies of LMP2-R/H, LMP2-R/R, LMP2-H/H were 47%, 47% and 6% in the IDDM patients, 13%, 87%, 0% in the controls. Compared with the Controls, the IDDM patients had a higher frequency of LMP2-R/H (P < 0.05) and a lower frequency of LMP2-R/R (P < 0.05). In the subjects without DR3 gene, the frequencies of LMP2-R/H, LMP2-R/R, LMP2-H/H were 58%, 39%, 2.8% respectively in the IDDM patients, and 32%, 54%, 14% respectively in the controls. Compared with the controls, the IDDM patients had a higher frequency of LMP2-R/H (P < 0.05), the frequency of LMP2-R/R in the IDDM patients did not have a significant difference from that in the controls (P > 0.05).


CONCLUSIONS
There may be a significant association of LMP2-R/H and LMP2-R/R with the susceptibility to IDDM. The LMP2-R/H is a susceptible gene. The people with LMP2-R/H have a higher risk of suffering from IDDM. The LMP2-R/R is a protective gene. The people with LMP2-R/R have a decreased risk of suffering from IDDM. The effects of LMP2 on ID-DM are not affected by DR3 gene.
Ding
H
H
Department of Endocrinology, Sun yat-sen Memorial Hospital, Zhong Shan University of Medical Sciences, Guang zhou 510120.
Cheng
H
H
Fu
Z
Z
D003546:Cysteine Endopeptidases; D003922:Diabetes Mellitus, Type 1 / Q000235:genetics*; D005260:Female; D015803:HLA-DR3 Antigen / Q000235:genetics; D006801:Humans; D008285:Major Histocompatibility Complex / Q000235:genetics; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D011506:Proteins / Q000235:genetics*
D015803:HLA-DR3 Antigen; D011506:Proteins; C071313:LMP-2 protein; D003546:Cysteine Endopeptidases