Oligomerization of the integrin alphaIIbbeta3: roles of the transmembrane and cytoplasmic domains.
Integrins are a family of alpha/beta heterodimeric membrane proteins, which mediate cell-cell and cell-matrix interactions. The molecular mechanisms by which integrins are activated and cluster are currently poorly understood. One hypothesis posits that the cytoplasmic tails of the alpha and beta subunits interact strongly with one another in a 1:1 interaction, and that this interaction is modulated in the course of the activation of alphaIIbbeta3 [Hughes, P. E., et al. (1996) J. Biol. Chem. 271, 6571-6574]. To examine the structural basis for this interaction, protein fragments encompassing the transmembrane helix plus cytoplasmic tails of the alpha and beta subunits of alphaIIbbeta3 were expressed and studied in phospholipid micelles at physiological salt concentrations. Analyses of these fragments by analytical ultracentrifugation, NMR, circular dichroism, and electrophoresis indicated that they had very little or no tendency to interact with one another. Instead, they formed homomeric interactions, with the alpha- and beta-fragments forming dimers and trimers, respectively. Thus, these regions of the protein structure may contribute to the clustering of integrins that accompanies cellular adhesion.
Li
R
R
Department of Biochemistry and Biophysics and Hematology-Oncology Division, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA.
Babu
C R
CR
Lear
J D
JD
Wand
A J
AJ
Bennett
J S
JS
DeGrado
W F
WF
D000595:Amino Acid Sequence; D002942:Circular Dichroism; D003593:Cytoplasm / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D009682:Magnetic Resonance Spectroscopy; D008565:Membrane Proteins / Q000737:chemistry*; D008823:Micelles; D008969:Molecular Sequence Data; D019039:Platelet Glycoprotein GPIIb-IIIa Complex / Q000737:chemistry*; D014462:Ultrafiltration
D008565:Membrane Proteins; D008823:Micelles; D019039:Platelet Glycoprotein GPIIb-IIIa Complex