title: Reprogramming of the macrophage transcriptome in response to interferon-gamma and Mycobacterium tuberculosis: signaling roles of nitric oxide synthase-2 and phagocyte oxidase.
abstract: Macrophage activation determines the outcome of infection by Mycobacterium tuberculosis (Mtb). Interferon-gamma (IFN-gamma) activates macrophages by driving Janus tyrosine kinase (JAK)/signal transducer and activator of transcription-dependent induction of transcription and PKR-dependent suppression of translation. Microarray-based experiments reported here enlarge this picture. Exposure to IFN-gamma and/or Mtb led to altered expression of 25% of the monitored genome in macrophages. The number of genes suppressed by IFN-gamma exceeded the number of genes induced, and much of the suppression was transcriptional. Five times as many genes related to immunity and inflammation were induced than suppressed. Mtb mimicked or synergized with IFN-gamma more than antagonized its actions. Phagocytosis of nonviable Mtb or polystyrene beads affected many genes, but the transcriptional signature of macrophages infected with viable Mtb was distinct. Studies involving macrophages deficient in inducible nitric oxide synthase and/or phagocyte oxidase revealed that these two antimicrobial enzymes help orchestrate the profound transcriptional remodeling that underlies macrophage activation.
authors.affiliation: Department of Microbiology and Immunology, Weill Medical College of Cornell University, NY 10021, USA.
authors.forename: S
authors.initials: S
authors.lastname: Ehrt
authors.forename: D
authors.initials: D
authors.lastname: Schnappinger
authors.forename: S
authors.initials: S
authors.lastname: Bekiranov
authors.forename: J
authors.initials: J
authors.lastname: Drenkow
authors.forename: S
authors.initials: S
authors.lastname: Shi
authors.forename: T R
authors.initials: TR
authors.lastname: Gingeras
authors.forename: T
authors.initials: T
authors.lastname: Gaasterland
authors.forename: G
authors.initials: G
authors.lastname: Schoolnik
authors.forename: C
authors.initials: C
authors.lastname: Nathan
mesh_terms: D000818:Animals; D005786:Gene Expression Regulation / Q000276:immunology*; D007371:Interferon-gamma / Q000276:immunology / Q000494:pharmacology; D008262:Macrophage Activation; D008264:Macrophages / Q000187:drug effects / Q000276:immunology* / Q000382:microbiology; D008562:Membrane Glycoproteins / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009169:Mycobacterium tuberculosis / Q000276:immunology; D000074662:NADPH Oxidase 2; D019255:NADPH Oxidases / Q000235:genetics / Q000276:immunology*; D019001:Nitric Oxide Synthase / Q000235:genetics / Q000276:immunology*; D052247:Nitric Oxide Synthase Type II; D020411:Oligonucleotide Array Sequence Analysis; D010586:Phagocytes / Q000201:enzymology; D015203:Reproducibility of Results; D015398:Signal Transduction / Q000276:immunology*; D014158:Transcription, Genetic / Q000276:immunology*
chemical_list: D008562:Membrane Glycoproteins; D007371:Interferon-gamma; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse; C056558:CYBB protein, human; D000074662:NADPH Oxidase 2; D019255:NADPH Oxidases