title: Ovine herpesvirus-2 glycoprotein B sequences from tissues of ruminant malignant catarrhal fever cases and healthy sheep are highly conserved.
abstract: Ovine herpesvirus-2 (OHV-2) infection has been associated with malignant catarrhal fever (MCF) in susceptible ruminants. In order to further investigate whether OHV-2 is an aetiological agent for sheep-associated (SA) MCF in cattle and bison, the entire sequences of OHV-2 glycoprotein B (gB) from different sources of viral DNA were compared. Target DNA was derived from tissues of bovine and bison cases of SA-MCF, from a lymphoblastoid cell line established from another bovine case of SA-MCF, and from a healthy sheep. The divergence between deduced amino acid sequences of OHV-2 gB ranged from 0.5 to 1.2%. The high degree of similarity between gB sequences from a healthy sheep and clinical cases of SA-MCF in cattle and bison suggests that OHV-2 is an ovine virus that is occasionally transmitted to other ruminant species, in which it can cause severe disease.
authors.affiliation: Department of Pathology, Colorado State University, Fort Collins, CO 80523, USA1.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Dunowska
authors.affiliation: USDA, ARS, Arthropod-borne Animal Diseases Research Laboratory, Laramie, WY 82071-3965, USA2.
authors.forename: Geoffrey J
authors.initials: GJ
authors.lastname: Letchworth
authors.affiliation: Department of Veterinary Science and Microbiology, University of Arizona, Tucson, AZ 85721-0090, USA3.
authors.forename: James K
authors.initials: JK
authors.lastname: Collins
authors.affiliation: Department of Pathology, Colorado State University, Fort Collins, CO 80523, USA1.
authors.forename: James C
authors.initials: JC
authors.lastname: DeMartini
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D016164:Bison / Q000821:virology; D002417:Cattle; D017124:Conserved Sequence; D006564:Herpesviridae / Q000235:genetics*; D008304:Malignant Catarrh / Q000821:virology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D012756:Sheep / Q000821:virology; D012757:Sheep Diseases / Q000821:virology*; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics*
chemical_list: C438803:Ovine herpesvirus-2 glycoprotein B; D014759:Viral Envelope Proteins