Nucleotide sequence analysis of a novel circovirus of canaries and its relationship to other members of the genus Circovirus of the family Circoviridae.
The circular, single-stranded DNA genome of a novel circovirus of canaries, tentatively named canary circovirus (CaCV), was cloned and sequenced. Sequence analysis indicated that the genome was 1952 nucleotides (nt) in size and had the potential to encode three viral proteins, including the putative capsid and replication-associated (Rep) proteins. The CaCV genome shared greatest sequence similarity (58.3% nt identity) with the newly characterized columbid circovirus (CoCV) and was more distantly related to the two porcine circovirus strains, PCV1 and PCV2, beak and feather disease virus (BFDV) and a recently isolated goose circovirus (GCV) isolate (46.8-50.9% nt identity). In common with other members of the Circovirus genus, several nt structures and amino acid motifs thought to be implicated in virus replication were identified on the putative viral strand. Phylogenetic analysis of both the capsid and Rep protein-coding regions provided further evidence that CaCV is more closely related to CoCV and BFDV and more distantly related to GCV, PCV1 and PCV2.
Phenix
Kerry V
KV
Division of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, Western Australia 6150, Australia1.
Weston
Jonathan H
JH
Department of Veterinary Science, The Queen's University of Belfast, Stormont, Belfast BT4 3SD, Northern Ireland2.
Ypelaar
Ingrid
I
Division of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, Western Australia 6150, Australia1.
Lavazza
Antonio
A
Electron Microscopy Laboratory, Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna, Via Bianchi 9, 25124 Brescia, Italy3.
Smyth
Joan A
JA
Department of Agriculture and Rural Development for Northern Ireland, Stormont, Belfast BT4 3SD, Northern Ireland4.
Todd
Daniel
D
Department of Agriculture and Rural Development for Northern Ireland, Stormont, Belfast BT4 3SD, Northern Ireland4.
Wilcox
Graham E
GE
Division of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, Western Australia 6150, Australia1.
Raidal
Shane R
SR
Division of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, Western Australia 6150, Australia1.
D000595:Amino Acid Sequence; D000818:Animals; D001715:Bird Diseases / Q000821:virology*; D002171:Canaries; D018136:Circoviridae / Q000145:classification / Q000235:genetics; D018173:Circoviridae Infections / Q000662:veterinary* / Q000821:virology; D017925:Circovirus / Q000145:classification* / Q000235:genetics; D016679:Genome, Viral; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA