Multiphoton excited fluorescence spectroscopy of biomolecular systems.
Recent work on the emerging application of multiphoton excitation to fluorescence studies of biomolecular dynamics and structure is reviewed. The fundamental principles and experimental techniques of multiphoton excitation are outlined, fluorescence lifetimes, anisotropy and spectra in membranes, proteins, hydrocarbons, skin, tissue and metabolites are featured, and future opportunities are highlighted.
Birch
D J
DJ
Department of Physics and Applied Physics, Strathclyde University, Glasgow, Scotland, UK. djs.birch@strath.ac.uk
D000473:Alkanes / Q000737:chemistry; D016880:Anisotropy; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000378:metabolism; D008543:Melanins / Q000737:chemistry; D017785:Photons; D011506:Proteins / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013053:Spectrophotometry; D013696:Temperature; D013997:Time Factors
D000473:Alkanes; D008164:Luminescent Proteins; D008543:Melanins; D011506:Proteins; D049452:Green Fluorescent Proteins