Molecular distribution sensing in a fluorescence resonance energy transfer based affinity assay for glucose.
A newly developed method for determining molecular distribution functions is applied to a widely researched glucose affinity sensor. The reduction in fluorescence resonance energy transfer (FRET) to a malachite green (MG)-dextran complex from allophycocyanin (APC) bound to concanavalin A (ConA) due to displacement of the complex by glucose from ConA provides the basis of the assay. The higher sensitivity and specificity of a new approach to fluorescence decay analysis, over the methods based on conventional Forster-type models, is demonstrated and critical parameters in competitive binding FRET sensing derived.
Rolinski
O J
OJ
Department of Physics and Applied Physics, University of Strathclyde, Glasgow, Scotland, UK.
Birch
D J
DJ
McCartney
L
L
Pickup
J C
JC
D003208:Concanavalin A / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D005947:Glucose / Q000032:analysis*; D007700:Kinetics; D015233:Models, Statistical; D010798:Phycocyanin / Q000737:chemistry; D012394:Rosaniline Dyes / Q000737:chemistry; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013997:Time Factors
D012394:Rosaniline Dyes; C002497:allophycocyanin; D010798:Phycocyanin; D003208:Concanavalin A; C005095:malachite green; D005947:Glucose