[Formation of an extracellular system of enzymes during growth of Geotrichum candidum 3C on cell walls isolated from cereal grain capsules].
The activities of extracellular systems of hemicellulases, pectinases, and cellulases was studied during a 72-h cultivation of Geotrichum candidum 3C. The culture was grown on a medium containing 3% cell walls isolated from wheat grain capsules, which served as the sole carbon source. Enzymes catalyzing the degradation of pectin substances (beet pectin, alpha-L-arabinan, and 1,4-beta-D-galactan), as well as beta-D-galactosidase and alpha-L-arabinofuranosidase involved in their hydrolysis, were formed first (4 h after the beginning of cultivation). Enzymes hydrolyzing 4-O-methyl-alpha-D-glucurono-beta-D-xylan and sodium carboxymethyl xylan were also found in the culture liquid after 4 h of fungal growth. The contents of pectin-degrading and xylanolytic enzymes reached their maximum levels after 52-56 and 72 h of growth, respectively. Cellulolytic enzymes were detected after 8-28 h of cultivation. Enzymes degrading alpha-D-galacto-beta-D-mannan were found 24 h after the beginning of growth; their content was maximum after 72 h of cultivation.
Rodionova
N A
NA
Bach Institute of Biochemistry, Russian Academy of Sciences, Leninskii pr. 33, Moscow, 117071 Russia.
Dubovaia
N V
NV
Martinovich
L I
LI
Bezborodov
A M
AM
D002473:Cell Wall / Q000201:enzymology / Q000382:microbiology*; D002480:Cellulase / Q000096:biosynthesis* / Q000378:metabolism; D002523:Edible Grain / Q000382:microbiology*; D005848:Geotrichum / Q000201:enzymology* / Q000254:growth & development; D006026:Glycoside Hydrolases / Q000096:biosynthesis* / Q000378:metabolism; D006868:Hydrolysis; D011096:Polygalacturonase / Q000096:biosynthesis* / Q000378:metabolism
D006026:Glycoside Hydrolases; C023305:hemicellulase; D011096:Polygalacturonase; D002480:Cellulase
Obrazovanie vnekletochnykkh sistem fermentov pri vyrashchivanii Geotrichum candiudum 3C na izolirovannykh kletchnykh stenkakh obolochek zerna zlakov