TAK1 activation of the mouse JunB promoter is mediated through a CCAAT box and NF-Y.
The JunB gene is activated by many stimuli including transforming growth factor beta (TGFbeta) family members and interleukin-6 (IL-6). Here the effect of TGFbeta activated kinase 1 (TAK1), a mitogen activated protein kinase kinase kinase (MAPKKK) implicated in TGFbeta, bone morphogenetic protein (BMP) and interleukin-1 (IL-1) signaling, on JunB promoter activity was investigated. Promoter analysis led to the identification of a CCAAT motif in the JunB gene, essential for activation by TAK1. Transfer of this CCAAT element to a heterologous minimal promoter conferred TAK1-responsiveness. The CCAAT-binding transcription factor, nuclear factor Y (NF-Y), activated the JunB promoter and a dominant negative NF-YA construct inhibited TAK1 activation of JunB. Our results demonstrate that JunB gene activation by TAK1 is mediated by the CCAAT-binding factor NF-Y.
Eggen
B J
BJ
Developmental Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kerklaan 30, 9751 NN, Haren, The Netherlands. b.j.l.eggen@biol.rug.nl
Benus
G F
GF
Folkertsma
S
S
Jonk
L J
LJ
Kruijer
W
W
D000818:Animals; D001483:Base Sequence; D023081:CCAAT-Binding Factor / Q000378:metabolism*; D002460:Cell Line; D004247:DNA; D017931:DNA Primers; D005786:Gene Expression Regulation / Q000235:genetics; D017930:Genes, Reporter; D006801:Humans; D007375:Interleukin-1 / Q000502:physiology; D008156:Luciferases / Q000235:genetics; D020930:MAP Kinase Kinase Kinases / Q000378:metabolism*; D051379:Mice; D011401:Promoter Regions, Genetic; D016755:Proto-Oncogene Proteins c-jun / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015533:Transcriptional Activation; D016212:Transforming Growth Factor beta / Q000502:physiology; D000099151:MAP Kinase Kinase Kinase 7
D023081:CCAAT-Binding Factor; D004247:DNA; D017931:DNA Primers; D007375:Interleukin-1; D008156:Luciferases; D020930:MAP Kinase Kinase Kinases; D016755:Proto-Oncogene Proteins c-jun; D016212:Transforming Growth Factor beta; D000099151:MAP Kinase Kinase Kinase 7