[Ectopic expression of human brain derived neurotrophic factor gene in fibroblasts].
The full-length sequence encoding hBDNF was inserted down into the enhancer-promotor regulatory element of Colia1 to construct minigene Colia1-BDNF. The recombinant eukaryotic expression plasmid pSCEPBFCAT was transfected into human fetal tendon fibroblasts in culture to realize ectopic expression of hBDNF gene in fibroblasts. The results of immuno-dot blot, Elisa and Western hybridization confirm that the expression protein with apparent molecular weight 27 kDa in pSCEPBFCAT transfected fibroblasts possesses antigen activity specific for the antibodies to BDNF.
Liu
Z
Z
Laboratory of Surgical Department, First Affiliated Hospital, West China University of Medical Sciences, Chengdu 610041.
Chen
J
J
Wang
R
R
Xiang
T
T
Li
X
X
Lin
J
J
You
L
L
Dong
Y
Y
D019208:Brain-Derived Neurotrophic Factor / Q000096:biosynthesis* / Q000235:genetics; D002478:Cells, Cultured; D005347:Fibroblasts / Q000378:metabolism*; D005786:Gene Expression Regulation; D005809:Genes, Regulator; D006801:Humans
D019208:Brain-Derived Neurotrophic Factor