Biosynthetic study of amphidinolide B.
The biosynthetic origins of amphidinoide B (1) were investigated on the basis of 13C-NMR data of 13C-enriched samples obtained by feeding experiments with [1-(13)C], [2-(13)C], and [1,2-(13)C2] sodium acetates in cultures of a dinoflagellate Amphidinium sp. These incorporation patterns suggested that 1 was generated from three successive polyketide chains, an isolated C1 unit from C-2 of acetates, six branched C1 units from C-2 of acetates, and an "m-m" and an "m-m-m" unit derived only from C-2 of acetates. The labeling patterns of amphidinolide B (1) were different from those of amphidinolide H (2), a 26-membered macrolide closely related to 1.
Tsuda
M
M
Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
Kubota
T
T
Sakuma
Y
Y
Kobayashi
J
J
D000085:Acetates / Q000378:metabolism; D000818:Animals; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000737:chemistry* / Q000302:isolation & purification; D004141:Dinoflagellida / Q000737:chemistry / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D000097442:Amphidinolides
D000085:Acetates; D019086:Bridged Bicyclo Compounds, Heterocyclic; C062197:amphidinolide D; D000097442:Amphidinolides