DNA replication in plants: characterization of a cdc6 homologue from Arabidopsis thaliana.
Cdc6 is a key regulator of DNA replication in eukaryotes. In this work, the expression pattern of an Arabidopsis cdc6 homologue is characterized by RT-PCR and in situ hybridization. The data suggest that cdc6At expression is cell cycle regulated. During development, high cdc6At mRNA levels are found in regular cycling cells. In addition, cdc6At expression is also observed in cells that are probably undergoing endoreduplication, suggesting a possible role of Cdc6At in this process in plants.
Ramos
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GB
Departamento de Bioquímica Médica, ICB, Universidade Federal do Rio de Janeiro, 21941-590, Rio de Janeiro, RJ, Brazil.
de Almeida Engler
J
J
Ferreira
P C
PC
Hemerly
A S
AS
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000502:physiology; D002453:Cell Cycle / Q000235:genetics / Q000502:physiology; D018797:Cell Cycle Proteins / Q000235:genetics*; D004261:DNA Replication; D018744:DNA, Plant / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014018:Tissue Distribution
D029681:Arabidopsis Proteins; C450459:CDC6 protein, Arabidopsis; D018797:Cell Cycle Proteins; D018744:DNA, Plant; D012333:RNA, Messenger