[Experimental study on the effect of combination therapy with lamivudine and famciclovir against duck hepatitis B virus in vivo].
OBJECTIVE
To study the antiviral effect of combination therapy with the nucleoside analog lamivudine and famciclovir on duck hepatitis virus (DHBV) in vivo.


METHODS
The Chongqing duck hepatitis B model was treated with lamivudine and famciclovir by intragastric administration for 4 weeks. DHBV DNA and DHBsAg in serum were observed by serum dot-blot hybridization and ELISA. ALT and AST in serum were also detected. Histological observation on the duck liver was done simultaneously. The trial was contrasted with a single acyclovir (ACV), famciclovir (FCV), or Lamivudine (3TC).


RESULTS
Combination therapy with Lamivudine and famciclovir could significantly reduce the serum DHBV DNA level. After stopping the treatment for 1 week, serum DHBV DNA level did not return significantly. The change of DHBsAg was similar to DHBV DNA. The level of ALT, AST, and the features of liver histopathology in combination-treated ducks were not different from those in control ducks.


CONCLUSIONS
The study confirms that combination therapy is superior to single antiviral agent in vivo for ducks with chronic DHBV carrier.
Chen
Y
Y
Institute for Viral Hepatitis, Chongqing University of Medical Sciences, Chongqing 400010, China.
Guo
S
S
Qi
Z
Z
Huang
A
A
D015075:2-Aminopurine / Q000031:analogs & derivatives* / Q000627:therapeutic use*; D000818:Animals; D000998:Antiviral Agents / Q000627:therapeutic use*; D004279:DNA, Viral / Q000097:blood; D004195:Disease Models, Animal; D004359:Drug Therapy, Combination; D004372:Ducks; D000077595:Famciclovir; D018347:Hepadnaviridae Infections / Q000188:drug therapy*; D015611:Hepatitis B Virus, Duck / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D006524:Hepatitis, Viral, Animal / Q000188:drug therapy*; D019259:Lamivudine / Q000627:therapeutic use*; D008826:Microbial Sensitivity Tests
D000998:Antiviral Agents; D004279:DNA, Viral; D019259:Lamivudine; D015075:2-Aminopurine; D000077595:Famciclovir