[CD59-blocked erythrocytes and hemolysis tests of paroxysmal nocturnal hemoglobinuria].
OBJECTIVE
To investigate the relationship between CD59 and traditional hemolysis tests for paroxysmal nocturnal hemoglobinuria.


METHODS
To make different concentrations of CD59-blocked erythrocytes by mixing with normal erythrocytes, and investigate the relationship between quantitative abnormalities of CD59 and hemolysis tests.


RESULTS
The hemolysis rate was positively related to the percentage of CD59-blocked erythrocytes. The greatest hemolysis of CD59-blocked erythrocytes was found in the CoF-initiated hemolysis test when the CD59-defects less than 16%, and the greater in Ham's test. When the CD59 defects were more than 16%, CD59-blocked erythrocytes hemolyzed to a greater extent in Ham's test than in the others.


CONCLUSIONS
Quantitative differences of CD59 underlies the variability in hemolysis tests, and the CoF-initiated hemolysis test is the most sensitive in detection of small CD59-defects.
Gao
J
J
Department of Hematology, Huashan Hospital, Shanghai Medical University, Shanghai 200040.
Xie
Y
Y
D000911:Antibodies, Monoclonal; D018957:CD59 Antigens / Q000276:immunology*; D004912:Erythrocytes / Q000276:immunology*; D006457:Hemoglobinuria, Paroxysmal / Q000097:blood*; D006461:Hemolysis; D006801:Humans
D000911:Antibodies, Monoclonal; D018957:CD59 Antigens