[Remnant abnormal clonal hematopoiesis in patients with paroxysmal nocturnal hemoglobinuria of clinical remission].
OBJECTIVE
To observe the abnormal clonal hematopoiesis status in patients with paroxysmal nocturnal hemoglobinuria(PNH) attained complete clinical remission(CCR).


METHODS
Immunofluorescence labeling and flow cytometry were used to detect the expression of CD59 in bone marrow mononuclear cells and peripheral red blood cell and the number of bone marrow CD34+ hematopoietic stem/progenitor cells.


RESULTS
In two patients in CCR, the coexistence of normal and abnormal hematopoiesis could still be found. However, the percentage of normal cells with CD59+ phenotype in peripheral red blood cells, bone marrow mononuclear cells and CD34+ hematopoietic stem/progenitor cells were significantly higher as compared with those in patients without remission, indicating that the normal hematopoiesis gradually regained dominance over the abnormal one when the disease approached remission.


CONCLUSIONS
In PNH patients in CCR, remnant abnormal (CD59-) cells could still be found in peripheral blood and bone marrow. It seems not necessary to climinate thoroughly all abnormal clones to achieve CCR in patients with PNH, and the dominance of abnormal hematopoicsis could vanish under some presently unknown mechanism.
Li
Q
Q
Department of Hematology, Peking Union Medical College Hospital, CAMS, PUMC, Beijing 100730.
Zhang
Z
Z
Lu
Z
Z
D018952:Antigens, CD34 / Q000276:immunology; D001854:Bone Marrow Cells / Q000473:pathology; D018957:CD59 Antigens / Q000276:immunology; D005260:Female; D006412:Hematopoietic Stem Cells / Q000473:pathology*; D006457:Hemoglobinuria, Paroxysmal / Q000097:blood*; D006801:Humans; D008875:Middle Aged; D012075:Remission, Spontaneous
D018952:Antigens, CD34; D018957:CD59 Antigens