title: Cloning and characterization of the metacyclogenin gene, which is specifically expressed during Trypanosoma cruzi metacyclogenesis.
abstract: We isolated a gene that is differentially expressed during Trypanosoma cruzi metacyclogenesis by the representation of differential expression (RDE) method, using differentiating epimastigotes cultured in chemically defined medium. This gene, the metacyclogenin gene, encodes a 630-nucleotide mRNA that is specifically associated with the polysomes of epimastigotes allowed to differentiate for 24 h. We sequenced and characterized the metacyclogenin gene and found that there were at least three copies of the gene organized into tandem 2.8 kb repeats in the genome of T. cruzi Dm28c. We analyzed the repeats and found that they contained two other genes, one encoding tryparedoxin peroxidase and the other encoding a 0.6 kb mRNA (named associated gene or AG) with sequences showing no significant similarity to those in the GenBank database. Northern blot analysis of polysomal RNA extracted from replicating and differentiating epimastigotes showed that metacyclogenin and AG genes displayed similar patterns of expression. Their products were detected only in differentiating epimastigotes, whereas tryparedoxin peroxidase was detected only in the polysomal RNA fraction of replicating and differentiating epimastigotes. In Northern blots of total RNA from differentiating and replicating epimastigotes, the genes studied were detected in both cell populations. The differential expression of the metacyclogenin gene was confirmed by immunocytochemistry studies showing that the protein is detected only in differentiating (adhered) epimastigote. The results suggest that mRNA mobilization to polysomes is an important mechanism in the regulation of gene expression in T. cruzi.
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Instituto Oswaldo Cruz, FIOCRUZ, Avenida Brasil 4365, Rio de Janeiro, RJ 21045-900, Brazil.
authors.forename: A R
authors.initials: AR
authors.lastname: Avila
authors.forename: S F
authors.initials: SF
authors.lastname: Yamada-Ogatta
authors.forename: V
authors.initials: V
authors.lastname: da Silva Monteiro
authors.forename: M A
authors.initials: MA
authors.lastname: Krieger
authors.forename: C V
authors.initials: CV
authors.lastname: Nakamura
authors.forename: W
authors.initials: W
authors.lastname: de Souza
authors.forename: S
authors.initials: S
authors.lastname: Goldenberg
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular / Q000379:methods*; D003470:Culture Media; D018076:DNA, Complementary / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D018503:Genome, Protozoan; D008018:Life Cycle Stages; D008969:Molecular Sequence Data; D020161:Physical Chromosome Mapping; D015800:Protozoan Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA; D014349:Trypanosoma cruzi / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*
chemical_list: D003470:Culture Media; D018076:DNA, Complementary; D015800:Protozoan Proteins; C445403:metacyclogenin, Trypanosoma cruzi