[Preparation, characterization and characteristics of copolypeptide consisting of L-aspartic acid and L-glutamic acid].
For the purpose of increasing the hydrophilicity of poly-aspartic acid, a copolypeptide consisting of L-aspartic acid and L-glutamic acid (8: 2 mol/mol) was prepared. The copolymer was characterized by 1H NMR, 13C NMR, differential scanning calorimetry, X-ray and element analysis. 3-hydroxyamino, as a side chain, was linked to the polymer, and poly-(3-hydroxypropyl)-L-aspartamide-L-glutamide (PHPAG) was obtained. The molecular weight of PHPAG was determined by GPC. Tests of the PHPAG on laboratory animals corroborated its potential use as a drug carrier. Acute toxicity studies revealed no death in animals treated, other studies recorded no notable difference between treated and control animals either in terms of principal haematological parameters or in micronucleus test. In vitro enzymatic hydrolysis for the co-polymer was performed using chymotrypsin, fungi protease and trypsin. It showed that these proteins could degrade the copolymer to some degree. The effects of pH, light and humidity on PHPAG were also investigated. The results indicated that it was stable under the experiment conditions.
Tang
G
G
School of Pharmacutical Science, Zhejiang University, Hangzhou 310031.
Zhu
Y
Y
Xie
X
X
Wu
Q
Q
D000818:Animals; D001224:Aspartic Acid; D001672:Biocompatible Materials; D004151:Dipeptides; D018698:Glutamic Acid; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism; D011099:Polyglutamic Acid / Q000031:analogs & derivatives* / Q000737:chemistry* / Q000378:metabolism; D051381:Rats; D013499:Surface Properties
D001672:Biocompatible Materials; D004151:Dipeptides; D010455:Peptides; D011099:Polyglutamic Acid; D001224:Aspartic Acid; D018698:Glutamic Acid; C063881:copoly(aspartic acid-glutamic acid)