title: Detection of species of the subgenus Leishmania parasites using polymerase chain reaction and southern blotting.
abstract: In this study, an attempt was made to identify different Leishmania species by polymerase chain reaction (PCR). Fourteen Leishmania strains from stock were tested by PCR and Southern blotting. A pair of primers were employed that anneal to the kinetoplast DNA sequence conserved among subgenus Leishmania. Of the 14 Leishmania strains used in this study, six showed strong bands of approximately 170 bp, and all the positive strains belonged to the species of the subgenus Leishmania viz., Leishmania (Leishmania) garnhami, L. (L.) amazonensis, L. (L.) pifanoi, L. (L.) mexicana, L. (L.) chagasi, and L. (L.) major All the species belonging to the subgenus Viannia used in this study were negative by PCR. These results suggest that the primer pair may be useful for identification of the species belonging to the subgenus Leishmania of the New World as well as to distinguish subgenus Leishmania from subgenus Viannia.
authors.affiliation: Department of Dermatology, School of Medicine, University of the Ryukyus, Okinawa, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Uezat
authors.forename: K
authors.initials: K
authors.lastname: Takei
authors.forename: M
authors.initials: M
authors.lastname: Marunp
authors.forename: N M
authors.initials: NM
authors.lastname: Khaskhel
authors.forename: S
authors.initials: S
authors.lastname: Nonak
authors.forename: M
authors.initials: M
authors.lastname: Oshiro
authors.forename: K I
authors.initials: KI
authors.lastname: Kariya
authors.forename: K
authors.initials: K
authors.lastname: Katakura
authors.forename: T
authors.initials: T
authors.lastname: Mimor
authors.forename: E A
authors.initials: EA
authors.lastname: Gomez
authors.forename: M
authors.initials: M
authors.lastname: Furuya
authors.forename: S M
authors.initials: SM
authors.lastname: Shamsuzzaman
authors.forename: Y
authors.initials: Y
authors.lastname: Hashiguch
mesh_terms: D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern / Q000379:methods*; D006801:Humans; D007891:Leishmania / Q000145:classification* / Q000302:isolation & purification*; D016773:Leishmaniasis, Cutaneous / Q000175:diagnosis; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity