Correlation of cell proliferation inhibition and apoptosis induction with expression of human beta 5 integrin on hematopoietic cells.
OBJECTIVE
To investigate the function of the alpha v beta 5 integrin in hematopoietic cells.


METHODS
Tissue culture, integrin expression vectors, gene transfer, polymerase chain reaction (PCR), apoptosis analyses and cytometic analysis were made on hematopoietic cells.


RESULTS
The beta 5 integrin cDNA was not expressed in hematopoietic cells following exposure to the beta 5 integrin retrovirus vector pG beta 5CHT. Unbalanced expression of the alpha v beta 3 and alpha v beta 5 integrins occurred during apoptosis induced by serum depletion and upon differentiation. The treatment of hematopoietic cells with anti-alpha v beta 5 monoclonal antibody inhibited apoptosis induced by serum depletion. Inducible expression of the beta 5 integrin cDNA in the hematopoietic cell line K562 caused cellular proliferation inhibition.


CONCLUSIONS
The alpha v beta 5 integrin cDNA in hematopoietic cells can inhibit the proliferation of the hematopoietic cell, cause the differentiation of the hematopoietic cells and induce the apoptosis of the hematopoietic cells.
Yin
L
L
Department of Pathophysiology, Shanghai Medical University, Shanghai 200032, China.
Fu
S
S
Zhao
X
X
Garcia-Sanchez
F
F
Deisseroth
A B
AB
D017209:Apoptosis / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D018014:Gene Transfer Techniques; D018922:HL-60 Cells / Q000473:pathology; D006412:Hematopoietic Stem Cells / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D039641:Integrin beta Chains; D016023:Integrins / Q000096:biosynthesis* / Q000235:genetics; D020014:K562 Cells / Q000473:pathology; D020298:U937 Cells
D039641:Integrin beta Chains; D016023:Integrins; C084344:integrin beta5