Solute adsorption and enzyme immobilization on chitosan beads prepared from shrimp shell wastes.
The equilibrium and kinetics of adsorption of reactive dye RR222 and Cu2+, and the activity of immobilization of acid phosphatase, on highly swollen chitosan beads were examined at 30 degrees C. The chitosan was prepared from shrimp shell wastes and was cross-linked with different dosages of glutaraldehyde or glyoxal (100-80,000 mg/l). It was shown that the amounts of solute adsorption and the immobilization capacity of acid phosphatase on cross-linked chitosan beads were substantially affected by their degree of cross-linking. The cross-linking rate of chitosan with glutaraldehyde could be described by a pseudo-second-order equation and the cross-linking equilibrium by the Freundlich equation. This provided an experimental method to control the degree of cross-linking of chitosan beads. Finally, the activity and lifetime of the immobilized enzyme were measured to evaluate the application potential.
Juang
R S
RS
Department of Chemical Engineering, Yuan Ze University, Chung-Li, Taiwan. cejuang@ce.yzu.edu.tw
Wu
F C
FC
Tseng
R L
RL
D000327:Adsorption; D000818:Animals; D002686:Chitin / Q000031:analogs & derivatives / Q000737:chemistry*; D048271:Chitosan; D033364:Decapoda; D004798:Enzymes / Q000737:chemistry; D005398:Fisheries; D005976:Glutaral / Q000737:chemistry; D006037:Glyoxal / Q000737:chemistry; D007700:Kinetics; D013997:Time Factors; D014865:Waste Disposal, Fluid; D018505:Waste Management / Q000379:methods
D004798:Enzymes; D002686:Chitin; D006037:Glyoxal; D048271:Chitosan; D005976:Glutaral