title: Revealing the involvement of extended hydrogen bond networks in the cooperative function between distant sites in bacterial reaction centers.
abstract: In reaction center proteins of photosynthetic bacteria, the amplitude of proton uptake induced by the one-electron reduction of either of the two quinone electron acceptors (Q(A) and Q(B)) is an intrinsic observable of the electrostatic interactions associated with the redox function of the complex. We report here that, in Rhodobacter capsulatus, complete restoration of proton uptake (upon formation of Q(A)(-) and Q(B)(-)) to the level found in the wild type is observed in a mutant reaction center in which a tyrosine substitution in the Q(A) environment (Ala(M274) --> Tyr) is coupled with mutations of acidic residues near Q(B) (Glu(L212) --> Ala/Asp(L213) --> Ala) that initially cancel the proton uptake above pH 8. This result demonstrates that proton uptake occurs by strong cooperation between structural motifs, such as hydrogen-bonded networks, that span the 18 A distance between the two quinone acceptors.
authors.affiliation: Centre de Génétique Moléculaire, CNRS, Gif 91198, France.
authors.forename: J
authors.initials: J
authors.lastname: Tandori
authors.forename: L
authors.initials: L
authors.lastname: Baciou
authors.forename: E
authors.initials: E
authors.lastname: Alexov
authors.forename: P
authors.initials: P
authors.lastname: Maroti
authors.forename: M
authors.initials: M
authors.lastname: Schiffer
authors.forename: D K
authors.initials: DK
authors.lastname: Hanson
authors.forename: P
authors.initials: P
authors.lastname: Sebban
mesh_terms: D006860:Hydrogen Bonding; D016296:Mutagenesis; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry* / Q000235:genetics; D016354:Rhodobacter capsulatus / Q000737:chemistry*
chemical_list: D045322:Photosynthetic Reaction Center Complex Proteins