title: Effect of extended immunosuppressive drug treatment on B cell vs T cell reconstitution in pediatric bone marrow transplant recipients.
abstract: Allogeneic bone marrow transplantation (BMT) is an effective therapy for a variety of malignancies and blood disorders, but rarely serves as a frontline treatment because of numerous, potential complications. Important and frequent complications relate to the profound immunosuppression that inevitably occurs during the first several months following treatment. To better elucidate and subsequently improve immune reconstitution, we examined T and B cell subsets among 43 pediatric BMT recipients in a retrospective study. We found that the relative numbers of T cells and B cells (T:B ratios) were discordant and highly variable among patients at day approximately 100 after BMT. Further investigation of BMT parameters identified a strong correlation between T:B ratios and immunosuppressive drug treatments, providing an explanation for variable lymphocyte reconstitution profiles. Results suggest that: (1) immunosuppressive therapy inhibits B cell expansion more strongly than T cell expansion following BMT; (2) WBC and absolute lymphocyte counts fail to reveal profound B cell immunodeficiencies in some BMT patients; and (3) routine analyses of T:B ratios serve to identify patients warranting close follow-up and extended supportive immunotherapy.
authors.affiliation: Department of Immunology, St Jude Children's Research Hospital, Memphis, TN 38105, USA.
authors.forename: S
authors.initials: S
authors.lastname: D'Costa
authors.forename: K S
authors.initials: KS
authors.lastname: Slobod
authors.forename: E
authors.initials: E
authors.lastname: Benaim
authors.forename: L
authors.initials: L
authors.lastname: Bowman
authors.forename: J
authors.initials: J
authors.lastname: Cunningham
authors.forename: M
authors.initials: M
authors.lastname: Holladay
authors.forename: N
authors.initials: N
authors.lastname: Howlett
authors.forename: D K
authors.initials: DK
authors.lastname: Srivastava
authors.forename: J L
authors.initials: JL
authors.lastname: Hurwitz
mesh_terms: D000305:Adrenal Cortex Hormones / Q000009:adverse effects / Q000627:therapeutic use; D018941:Antigens, CD19 / Q000097:blood; D001402:B-Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D016026:Bone Marrow Transplantation; D017252:CD3 Complex / Q000097:blood; D002648:Child; D006402:Hematologic Diseases / Q000628:therapy; D006410:Hematopoiesis / Q000187:drug effects; D006801:Humans; D007166:Immunosuppressive Agents / Q000627:therapeutic use*; D018655:Lymphocyte Count; D012189:Retrospective Studies; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D013997:Time Factors; D014184:Transplantation, Homologous
chemical_list: D000305:Adrenal Cortex Hormones; D018941:Antigens, CD19; D017252:CD3 Complex; D007166:Immunosuppressive Agents