[Expression of a cloned hemagglutinin-neuraminidase gene from Newcastle disease virus in murine myeloma cells].
Vaccination with autologous cancer cells expressing a potent foreign antigen is promising for immunotherapy of tumors. A construct was obtained to transfect cancer cells with the hemagglutinin-neuraminidase (HN) gene of the Newcastle disease virus (NDV). Specific primers were designed, and the HN cDNA was amplified from RNA isolated from the allantoid fluid of NDV-infected embryonated chicken eggs. The amplified fragment was cloned in pCR2.1, sequenced, and recloned in expression vector pCDNA3.1/Zeo(+). The resulting construct was used to transfect mouse myeloma cells SP2/0. Production of HN was checked by ELISA and by a neuraminidase activity assay. Cell agglutination on ice was proposed as a test for surface HN.
Risinskaia
N V
NV
risinska@blood.ru
Fegeding
K V
KV
Vasilenko
O V
OV
Sudarikov
A B
AB
D000818:Animals; D001483:Base Sequence; D019496:Cancer Vaccines / Q000008:administration & dosage; D003001:Cloning, Molecular; D017931:DNA Primers; D006389:Hemagglutinins, Viral / Q000235:genetics*; D051379:Mice; D009101:Multiple Myeloma / Q000235:genetics* / Q000821:virology; D009439:Neuraminidase / Q000235:genetics*; D009522:Newcastle disease virus / Q000201:enzymology / Q000235:genetics*; D014407:Tumor Cells, Cultured
D019496:Cancer Vaccines; D017931:DNA Primers; D006389:Hemagglutinins, Viral; D009439:Neuraminidase
Ekspressiia klonirovannogo gena gemaggliutinin-neĭraminidazy virusa bolezni N'iukasla v kletkakh myshinoĭ mielomy.