A monoclonal antibody specific for Pseudomonas aeruginosa amidase.
Amidase from Pseudomonas aeruginosa was purified by anionic exchange chromatography and used to immunise female Balb/c mice. Monoclonal antibodies (MAbs) were raised by hybridoma technology using Sp2/0 myeloma cells as fusion partner. A selected IgM subclass MAb was purified from in vitro hybridoma cell line supernatant by a two-step anionic exchange chromatography. The MAb was specific for amidase from P. aeruginosa as determined by Western blotting and recognized the native and denatured forms of the enzyme.
Novo
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C
INETI, Departmento de Biotecnologia, UTPAM, Estrada do Paço do Lumiar, 1649-038, Lisboa, Portugal. carlos.novo@mail.ineti.pt
Karmali
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A
Clemente
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A
Brown
P R
PR
D000581:Amidohydrolases / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D000918:Antibody Specificity; D015153:Blotting, Western; D002852:Chromatography, Ion Exchange; D005260:Female; D007075:Immunoglobulin M / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D011550:Pseudomonas aeruginosa / Q000201:enzymology*
D000911:Antibodies, Monoclonal; D007075:Immunoglobulin M; D000581:Amidohydrolases; C019665:amidase