title: Nucleotide sequence analysis of a novel circovirus of canaries and its relationship to other members of the genus Circovirus of the family Circoviridae.
abstract: The circular, single-stranded DNA genome of a novel circovirus of canaries, tentatively named canary circovirus (CaCV), was cloned and sequenced. Sequence analysis indicated that the genome was 1952 nucleotides (nt) in size and had the potential to encode three viral proteins, including the putative capsid and replication-associated (Rep) proteins. The CaCV genome shared greatest sequence similarity (58.3% nt identity) with the newly characterized columbid circovirus (CoCV) and was more distantly related to the two porcine circovirus strains, PCV1 and PCV2, beak and feather disease virus (BFDV) and a recently isolated goose circovirus (GCV) isolate (46.8-50.9% nt identity). In common with other members of the Circovirus genus, several nt structures and amino acid motifs thought to be implicated in virus replication were identified on the putative viral strand. Phylogenetic analysis of both the capsid and Rep protein-coding regions provided further evidence that CaCV is more closely related to CoCV and BFDV and more distantly related to GCV, PCV1 and PCV2.
authors.affiliation: Division of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, Western Australia 6150, Australia1.
authors.forename: Kerry V
authors.initials: KV
authors.lastname: Phenix
authors.affiliation: Department of Veterinary Science, The Queen's University of Belfast, Stormont, Belfast BT4 3SD, Northern Ireland2.
authors.forename: Jonathan H
authors.initials: JH
authors.lastname: Weston
authors.affiliation: Division of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, Western Australia 6150, Australia1.
authors.forename: Ingrid
authors.initials: I
authors.lastname: Ypelaar
authors.affiliation: Electron Microscopy Laboratory, Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna, Via Bianchi 9, 25124 Brescia, Italy3.
authors.forename: Antonio
authors.initials: A
authors.lastname: Lavazza
authors.affiliation: Department of Agriculture and Rural Development for Northern Ireland, Stormont, Belfast BT4 3SD, Northern Ireland4.
authors.forename: Joan A
authors.initials: JA
authors.lastname: Smyth
authors.affiliation: Department of Agriculture and Rural Development for Northern Ireland, Stormont, Belfast BT4 3SD, Northern Ireland4.
authors.forename: Daniel
authors.initials: D
authors.lastname: Todd
authors.affiliation: Division of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, Western Australia 6150, Australia1.
authors.forename: Graham E
authors.initials: GE
authors.lastname: Wilcox
authors.affiliation: Division of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, Western Australia 6150, Australia1.
authors.forename: Shane R
authors.initials: SR
authors.lastname: Raidal
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001715:Bird Diseases / Q000821:virology*; D002171:Canaries; D018136:Circoviridae / Q000145:classification / Q000235:genetics; D018173:Circoviridae Infections / Q000662:veterinary* / Q000821:virology; D017925:Circovirus / Q000145:classification* / Q000235:genetics; D016679:Genome, Viral; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA