Liquid chromatographic analysis of vitamin B6 in soy-based infant formula.
A liquid chromatographic (LC) method is described for determination of total vitamin B6 in soy-based infant formula. Total vitamin B6 is quantitated by using ion-pair LC after precolumn transformation of phosphorylated and free vitamers into pyridoxol. The limit of detection is 0.3 ng and the limit of quantitation is 1.0 ng on-column (injection volume = 100 microL). Linear response ranged from 39 to 616 ng/mL (r2 = 0.99986). Analysis of a soy-based infant formula control fortified at 6 different concentration levels gave recoveries that averaged 104%. Assay of SRM 1846 gave results within the certified range (8.6 +/- 0.086 mg/kg versus the certified value of 8.4 +/- 1.0 mg/kg). The method provides a rapid and specific assay for the analysis of total vitamin B6 in fortified soy-based infant formula.
Mann
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DL
U.S. Food and Drug Administration, Southeast Regional Laboratory, Atlanta, GA 30309, USA.
Chase
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GW
Eitenmiller
R R
RR
D002138:Calibration; D002853:Chromatography, Liquid; D006801:Humans; D007202:Indicators and Reagents; D007223:Infant; D007225:Infant Food / Q000032:analysis*; D013025:Glycine max / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D014465:Ultrasonics; D025101:Vitamin B 6 / Q000031:analogs & derivatives / Q000032:analysis*
D007202:Indicators and Reagents; D025101:Vitamin B 6