[The effect of human cytomegalovirus on human marrow granulocyte/macrophage progenitor].
OBJECTIVE
To investigate whether the suppression of CFU-GM is caused directly by human cytomegalovirus(HCMV).


METHODS
The effect of HCMV AD 169 strain on CFU-GM growth was assayed in vitro and HCMV AD 169 DNA in CFU-GM was detected by in situ PCR(ISPCR).


RESULTS
Significant inhibition of CFU-GM was observed when HCMV AD 169 containing fluid was diluted to 2 x 10(5) pfu/ml and 2 x 10(6) pfu/ml (P < 0.01). The suppression was in a dose-dependent fashion. HCMV AD 169-DNA was detected by ISPCR on the nuclear of CFU-GM.


CONCLUSIONS
HCMV might cause suppression of CFU-GM growth by direct infection.
Xiong
W
W
Third Teaching Hospital, Sun Yat-sen University of Medical Sciences, Guangzhou 510630.
He
Z
Z
Wang
Q
Q
D000293:Adolescent; D000328:Adult; D002455:Cell Division; D003587:Cytomegalovirus / Q000472:pathogenicity*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D023461:Myeloid Progenitor Cells / Q000166:cytology* / Q000821:virology