title: UV-irradiation-induced DNA immobilization and functional utilization of DNA on nonwoven cellulose fabric.
abstract: Immobilization of double-stranded DNA onto nonwoven cellulose fabric by UV irradiation and utilization of DNA-immobilized cloth were examined. The immobilized DNA was found to be stable in water, with the maximum amount of fabric-immobilized DNA being approximately 20 mg/g of nonwoven fabric. The DNA-immobilized cloth could effectively accumulate endocrine disruptors and harmful DNA intercalating pollutants, such as dibenzo-p-dioxin, dibenzofuran, biphenyl, benzo[a]pyrene and ethidium bromide. Additionally, DNA-immobilized cloth was found to bind metal ions, such as Ag+, Cu2+, and Zn2+. The maximum amounts of bound Ag+, Cu2+, and Zn2+ onto DNA-immobilized cloth (1 g) were approximately 5, 2, and 1 mg, respectively. DNA-immobilized cloth containing Ag+ showed antibacterial activity against Escherichia coli and Staphylococcus aureus. DNA-immobilized cloth without metal ion and with Cu2+ or Zn2+ did not show antibacterial activity. These results suggest that immobilized DNA imparts useful functionality to cloth. DNA-immobilized cloth prepared by UV irradiation has potential to serve as a useful biomaterial for medical, engineering, and environmental application.
authors.affiliation: Laboratory of Bio-Material Chemistry, Division of Bioscience, Graduate School of Environmental Earth Science, Hokkaido University, Sapporo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Yamada
authors.forename: K
authors.initials: K
authors.lastname: Kato
authors.forename: K
authors.initials: K
authors.lastname: Shindo
authors.forename: M
authors.initials: M
authors.lastname: Nomizu
authors.forename: M
authors.initials: M
authors.lastname: Haruki
authors.forename: N
authors.initials: N
authors.lastname: Sakairi
authors.forename: K
authors.initials: K
authors.lastname: Ohkawa
authors.forename: H
authors.initials: H
authors.lastname: Yamamoto
authors.forename: N
authors.initials: N
authors.lastname: Nishi
mesh_terms: D002482:Cellulose; D004247:DNA; D004702:Endocrine Glands / Q000187:drug effects; D004926:Escherichia coli / Q000254:growth & development; D011078:Polychlorinated Biphenyls / Q000494:pharmacology; D013211:Staphylococcus aureus / Q000254:growth & development; D014466:Ultraviolet Rays
chemical_list: D002482:Cellulose; D004247:DNA; D011078:Polychlorinated Biphenyls