Monitoring biogenic volatile compounds emitted by Eucalyptus citriodora using SPME.
A procedure to monitor BVOC emitted by living plants using SPME technique is presented. For this purpose, a glass sampling chamber was designed. This device was employed for the characterization of biogenic volatile compounds emitted by leaves of Eucalyptus citriodora. After extraction with SPME fibers coated with PDMS/ DVB, it was possible to identify or detect 33 compounds emitted by this plant. A semiquantitative approach was applied to monitor the behavior of the emitted BVOC during 9 days. Circadian profiles of the variation in the concentration of isoprene were plotted. Using diffusion-based SPME quantitation, a recently introduced analytical approach, with extraction times as short as 15 s, it was possible to quantify subparts-per-billion amounts of isoprene emitted by this plant.
Zini
C A
CA
Department of Chemistry, University of Waterloo, Ontario, Canada.
Augusto
F
F
Christensen
T E
TE
Smith
B P
BP
Caramão
E B
EB
Pawliszy
J
J
D002070:Butadienes / Q000032:analysis / Q000378:metabolism; D002623:Chemistry Techniques, Analytical / Q000379:methods; D002849:Chromatography, Gas / Q000379:methods; D002940:Circadian Rhythm; D005052:Eucalyptus / Q000378:metabolism*; D045782:Hemiterpenes; D009930:Organic Chemicals / Q000032:analysis / Q000378:metabolism*; D010420:Pentanes; D010946:Plants, Medicinal; D014835:Volatilization
D002070:Butadienes; D045782:Hemiterpenes; D009930:Organic Chemicals; D010420:Pentanes; C005059:isoprene