Antioxidant activity of qizhu tang.
OBJECTIVE
To study the antioxidant, activity of Qizhu Tang (QZT) both in vivo and in vitro.


METHODS
QZT consists of 4 herbal constituents (Rhizoma Atractylodis Macrocephalae, Poria cocos, Kadix Notoginseng, and Radix Astragali), each of the components and their combinations were examined in vitro for 1, 1-diphenyl-2-picrylhydrazyl (DPPH) and hydroxyl radical scavenging activities, and for the inhibition of thiobarbituric acid-reactive substances (TBARS) formation in rat liver homogenate. At the same time, their in vivo protective effect on cerebral ischemia-areperfusion injury was determined in rats.


RESULTS
Only the preparations having a higher antioxidant activity comparable to QZT in all three in vitro assays were relatively active in vivo both for TBARS inhibition and glutathione peroxidase preservation, although the activities were much lower than that of QZT as a whole.


CONCLUSIONS
QZT formula is a good natural antioxidant having an effective preventive effect against cerebral ischemia reperfusion damage.
Wang
X J
XJ
Beijing Union University, Traditional Chinese Medicine and Pharmacology College, Beijing 100013, China. Wang-xj@netease.com
Feng
P
P
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002545:Brain Ischemia / Q000150:complications; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000494:pharmacology; D005979:Glutathione Peroxidase / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000201:enzymology / Q000209:etiology / Q000517:prevention & control*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D000975:Antioxidants; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D016166:Free Radical Scavengers; D017392:Thiobarbituric Acid Reactive Substances; D005979:Glutathione Peroxidase