Mutagenesis in the post genomics era: tools for generating insertional mutations in the lactic acid bacteria.
The increasing availability of whole genome sequences has increased the demand for effective tools to generate insertional mutations in the lactic acid bacteria (LAB). Several novel approaches, such as shuttle-, transposome- and intron-based mutagenesis methods, are possible additions to the existing repertoire of transposon- and recombination-based tools available for mutagenesis of LAB.
Mills
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Department of Viticulture and Enology, University of California, 1 Shields Avenue, Davis, CA 95616-8749, USA. damills@ucdavis.edu
D001419:Bacteria / Q000235:genetics*; D002876:Chromosomes, Bacterial; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics; D005524:Food Technology; D016680:Genome, Bacterial; D023281:Genomics; D007438:Introns; D019344:Lactic Acid / Q000378:metabolism; D016254:Mutagenesis, Insertional / Q000379:methods*; D016297:Mutagenesis, Site-Directed; D011995:Recombination, Genetic
D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D019344:Lactic Acid