CD34(+) cell collection efficiency does not correlate with the pre-leukapheresis hematocrit.
One hundred and seventy-seven large-volume leukapheresis procedures performed on 91 patients over a 15 month period were reviewed to see if the pre-apheresis hematocrit (Hct) affected the CD34(+) cell collection efficiency (CE) of the Fenwal CS 3000 Plus cell separator. The Hct was 0.174-0.461 (median 0.317), and the peripheral blood CD34(+) cell count 2-2487 per microl (median 21). The total CD34(+) cell quantity collected was 3.0-2677.2 x 10(6) (median 113.0). Based on the number of CD34(+)cells contained in the blood volume processed (23.3-37303.2 x 10(6); median 318.0), the CE was 1.7-87.5% (median 30.3). No correlation was found between the Hct and CE (r(2) = 0.0034; P = 0.44) or the total CD34(+) cell quantity collected (r2 = 0.0040; P = 0.40). CEs for Hct <0.25 (median CE 36%), Hct 0.25-0.299 (median CE 30%) and Hct 0.30 (median CE 30%) were comparable. As expected, highly significant correlations were seen between the CD34(+) cell quantities collected and quantities processed (r2 = 0.59; P < 10(-6)) as well as the peripheral blood CD34(+) cell counts (r2= 0.60; P < 10(-6)). We conclude that the minimum acceptable Hct or hemoglobin level for leukapheresis should be dictated by clinical circumstances because it does not affect stem cell collection.
Mehta
J
J
The Hematopoietic Stem Cell Transplant Program, Division of Hematology/Oncology, Department of Internal Medicine, Northwestern University Medical School and The Robert H Lurie Comprehensive Cancer Center of Northwestern University, Chicago, IL 60611, USA.
Oyama
Y
Y
Winter
J
J
Williams
S
S
Tallman
M
M
Singhal
S
S
Villa
M
M
Shook
T
T
Burt
R
R
Traynor
A
A
Soff
G
G
Masarik
S
S
Ramsey
G
G
Gordon
L
L
D018952:Antigens, CD34 / Q000097:blood*; D006400:Hematocrit; D006402:Hematologic Diseases / Q000628:therapy; D006454:Hemoglobins / Q000378:metabolism; D006801:Humans; D007937:Leukapheresis / Q000592:standards*; D018655:Lymphocyte Count; D012044:Regression Analysis; D012189:Retrospective Studies
D018952:Antigens, CD34; D006454:Hemoglobins