title: The AP-1 repressor, JDP2, is a bona fide substrate for the c-Jun N-terminal kinase.
abstract: The Jun dimerization protein 2 (JDP2) is a novel member of the basic leucine zipper family of transcription factors. JDP2 binds DNA as a homodimer and heterodimer with ATF2 and Jun proteins but not with c-Fos proteins. JDP2 overexpression represses activating protein 1 transcription activity. Whereas JDP2 mRNA and protein levels are stable following different cell stimuli, JDP2 is rapidly phosphorylated upon UV irradiation, oxidative stress and low levels of translation inhibitor. The c-Jun N-terminal kinase phosphorylates JDP2 both in vitro and in vivo. JDP2 contains a putative consensus JNK docking-site and a corresponding phosphoacceptor site. Substitution of threonine 148 to an alanine residue blocks JNK-dependent JDP2 phosphorylation. Our data indicate that JDP2 is a bona fide substrate for the c-Jun N-terminal kinase. The precise role of JDP2 phosphorylation on its function is not yet known.
authors.affiliation: Department of Molecular Genetics and the Rappaport Family Institute for Research in the Medical Sciences and the B. Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, P.O. Box 9649, Bat-Galim, 31096, Haifa, Israel.
authors.forename: S
authors.initials: S
authors.lastname: Katz
authors.forename: R
authors.initials: R
authors.lastname: Heinrich
authors.forename: A
authors.initials: A
authors.lastname: Aronheim
mesh_terms: D000818:Animals; D002460:Cell Line; D048031:JNK Mitogen-Activated Protein Kinases; D051379:Mice; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D010766:Phosphorylation; D051381:Rats; D012097:Repressor Proteins / Q000378:metabolism*; D013379:Substrate Specificity; D013912:Threonine / Q000378:metabolism
chemical_list: C106160:Jdp2 protein, rat; C491386:Jundp2 protein, mouse; D012097:Repressor Proteins; D013912:Threonine; D048031:JNK Mitogen-Activated Protein Kinases; D020928:Mitogen-Activated Protein Kinases