title: Rapid enantiomeric quantification of an antiviral nucleoside agent (D,L-FMAU, 2'-fluoro-5-methyl-beta,D,L-arabinofurano-syluracil) by mass spectrometry.
abstract: A novel mass spectrometric method is applied to rapid, accurate (<1%) quantification of chiral Clevudine (L-FMAU, 2'-fluoro-5-methyl-beta,L-arabinofuranosyluracil), a potent antiviral nucleoside agent against hepatitis B virus. Transition metal bound complex ions containing the chiral drug are generated by electrospray ionization mass spectrometry and subjected to collision-induced dissociation. The ratio of the two competitive dissociation rates is related to the enantiomeric composition of the drug mixture, allowing the determination of enantiomeric contamination in the drug.
authors.forename: W A
authors.initials: WA
authors.lastname: Tao
authors.forename: L
authors.initials: L
authors.lastname: Wu
authors.forename: R G
authors.initials: RG
authors.lastname: Cooks
authors.forename: F
authors.initials: F
authors.lastname: Wang
authors.forename: J A
authors.initials: JA
authors.lastname: Begley
authors.forename: B
authors.initials: B
authors.lastname: Lampert
mesh_terms: D000998:Antiviral Agents / Q000737:chemistry*; D001086:Arabinofuranosyluracil / Q000031:analogs & derivatives / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D013237:Stereoisomerism
chemical_list: D000998:Antiviral Agents; D001086:Arabinofuranosyluracil; C034935:clevudine