Certification of the mass concentration of creatine kinase isoenzyme 2 (CK-MB) in the reference material BCR 608.
We describe the certification of a mass concentration value in the already prepared creatine kinase-2 reference material (BCR 608). Creatine kinase-2 was purified from human heart. The purified enzyme was diluted in order to measure its protein concentration by the Doetsch method. A protein concentration value of 124.30+/-13.17 mg/l was assigned to the stock solution of purified creatine kinase-2. This stock solution was diluted in 25 mmol/l piperazine-N,N'-bis[2-ethanesulfonic acid] (PIPES) pH 7.2, containing 2 mmol/l ADP, 5 mmol/l 2-mercaptoethanol, 154 mmol/l sodium chloride and 50 g/l human albumin to obtain a stable liquid standard of known creatine kinase-2 mass concentration (80.36 microg/l). This standard was then used to recalculate the creatine kinase-2 mass concentration measured in the BCR 608 material by immunoassay. The mass concentration of creatine kinase-2 in samples of reconstituted BCR 608 was certified to be 93.30+/-9.65 microg/l.
Sánchez
M
M
Departament de Bioquimica i de Biologia Molecular, Unitat de Bioquímica de Medicina, Universitat Autònoma de Barcelona, Bellaterra, Spain.
Gella
F J
FJ
Profilis
C
C
Ceriotti
F
F
Cusó
E
E
Fuentes-Arderiu
X
X
Hallermayer
K
K
Hørder
M
M
Panteghini
M
M
Schumann
G
G
Canalias
F
F
D002138:Calibration; D002568:Certification; D002852:Chromatography, Ion Exchange; D003402:Creatine Kinase / Q000032:analysis*; D052279:Creatine Kinase, MB Form; D004591:Electrophoresis, Polyacrylamide Gel; D004795:Enzyme Stability; D006801:Humans; D007527:Isoenzymes / Q000032:analysis*; D009206:Myocardium / Q000201:enzymology; D012015:Reference Standards; D012016:Reference Values
D007527:Isoenzymes; D003402:Creatine Kinase; D052279:Creatine Kinase, MB Form