Congeners of SMAP29 kill ovine pathogens and induce ultrastructural damage in bacterial cells.
SMAP29, an ovine cathelicidin, was systematically altered to create a family of 23 related peptides for MIC and minimum bactericidal concentration determinations. SMAP28, SMAP29, and a derivative of SMAP29 called ovispirin were all antimicrobial. However, many congeners of SMAP29 and ovispirin were not as active as the parent molecules. With immunoelectron microscopy, SMAP29 was seen on membranes and within the cytoplasm of Pseudomonas aeruginosa PAO1.
Kalfa
V C
VC
Respiratory Diseases of Livestock Research Unit, USDA Agricultural Research Service, National Animal Disease Center, Ames, Iowa 50010, USA.
Jia
H P
HP
Kunkle
R A
RA
McCray
P B
PB
Tack
B F
BF
Brogden
K A
KA
D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects* / Q000648:ultrastructure*; D001798:Blood Proteins / Q000494:pharmacology*; D054804:Cathelicidins; D008826:Microbial Sensitivity Tests; D016253:Microscopy, Immunoelectron; D008969:Molecular Sequence Data; D012756:Sheep / Q000382:microbiology*; D012757:Sheep Diseases / Q000382:microbiology*
D000900:Anti-Bacterial Agents; D001798:Blood Proteins; D054804:Cathelicidins; C403305:SMAP29 protein, Ovis aries