Studies on the antibody response of Lama glama--evaluation of the binding capacity of different IgG subtypes in ELISAs for clenbuterol and BSA.
Camelidae are known to produce three subtypes of immunoglobulin G (IgG), two of which are devoid of light chains. Two llamas (Lama glama) were immunised against clenbuterol-bovine serum albumin (BSA). Enzyme-linked immunosorbent assays (ELISAs) for clenbuterol and BSA on the basis of protein A-coated microtitration plates were established to investigate the titre development. Three subclasses of IgG (IgG(1): 29+66KDD, IgG(2): 52KDD, IgG(3): 56KDD) depending on their different binding properties to protein A and protein G could be separated chromatographically. Only IgG(1), which consists of conventional four-chain antibodies, bound to clenbuterol, whereas all forms of heavy-chain antibodies merely bound BSA.
Lange
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Institute of Physiology, Technical University Munich-Weihenstephan, Weihenstephaner Berg 3, D-85354 Freising, Germany. lange@weihenstephan.de
Daxenberger
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A
Meyer
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D000318:Adrenergic beta-Agonists / Q000276:immunology*; D000818:Animals; D015153:Blotting, Western / Q000662:veterinary; D002161:Camelids, New World / Q000276:immunology*; D002846:Chromatography, Affinity / Q000662:veterinary; D002976:Clenbuterol / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D007136:Immunoglobulins / Q000032:analysis / Q000096:biosynthesis*; D008297:Male; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine / Q000276:immunology*; D013205:Staphylococcal Protein A / Q000276:immunology
D000318:Adrenergic beta-Agonists; D007136:Immunoglobulins; D013205:Staphylococcal Protein A; D012710:Serum Albumin, Bovine; D002976:Clenbuterol