Characterization of the lipA gene encoding the major lipase from Pseudomonas aeruginosa strain IGB83.
The lipases produced by Pseudomonas have a wide range of potential biotechnological applications. Pseudomonas aeruginosa IGB83 was isolated as a highly lipolytic strain which produced a thermotolerant and alkaline lipase. In the present work, we have characterized the P. aeruginosa IGB83 gene (lipA) encoding this enzyme. We describe the construction of a lipA mutant and report on the effect of two carbon sources on lipase expression.
Martínez
A
A
Departamento de Microbiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de Mexico, Cuernavaca, Morelos.
Soberón-Chávez
G
G
D001426:Bacterial Proteins; D002244:Carbon / Q000378:metabolism; D002265:Carboxylic Ester Hydrolases / Q000235:genetics* / Q000378:metabolism*; D005798:Genes, Bacterial; D008049:Lipase; D008969:Molecular Sequence Data; D009154:Mutation; D011550:Pseudomonas aeruginosa / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D017422:Sequence Analysis, DNA
D001426:Bacterial Proteins; D002244:Carbon; D002265:Carboxylic Ester Hydrolases; C113518:LipA protein, Pseudomonas; D008049:Lipase