title: MSH2 and MLH1 immunodetection and the prognosis of colon cancer.
abstract: MSH2 and MLH1 are proteins involved in DNA reparation. They are mutated in some forms of colon cancer, i.e. hereditary non-polyposis carcinomas and a subset of sporadic carcinomas. We have studied the expression of MSH2 and MLH1 in a retrospective series of 225 colorectal carcinomas by immunohistochemistry. The results were compared to molecular tests of microsatellite instability using amplification by PCR of BAT-25 and BAT-26 repeated sequences and to histoclinical data. Positivity of both proteins was never associated with MSI phenotype. MSH2 and/or MLH1 negative tumors were frequently tumors of the proximal colon; in this subpopulation or proximal tumors, MSH2 and/or MLH1 negativity was associated with a longer disease-free survival.
authors.affiliation: Departement d'Oncologie Moleculaire, Institut Paoli-Calmettes (IPC), Universite de la Mediterranee, Marseille Cedex 9, France.
authors.forename: J
authors.initials: J
authors.lastname: Perrin
authors.forename: J
authors.initials: J
authors.lastname: Gouvernet
authors.forename: D
authors.initials: D
authors.lastname: Parriaux
authors.forename: T
authors.initials: T
authors.lastname: Noguchi
authors.forename: M H
authors.initials: MH
authors.lastname: Giovannini
authors.forename: M
authors.initials: M
authors.lastname: Giovannini
authors.forename: J R
authors.initials: JR
authors.lastname: Delpero
authors.forename: D
authors.initials: D
authors.lastname: Birnbaum
authors.forename: G
authors.initials: G
authors.lastname: Monges
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000368:Aged; D020137:Base Pair Mismatch; D002352:Carrier Proteins; D003110:Colonic Neoplasms / Q000737:chemistry* / Q000175:diagnosis*; D004260:DNA Repair; D004273:DNA, Neoplasm / Q000032:analysis; D004268:DNA-Binding Proteins; D018572:Disease-Free Survival; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D018895:Microsatellite Repeats; D000070957:MutL Protein Homolog 1; D051718:MutS Homolog 2 Protein; D009363:Neoplasm Proteins / Q000032:analysis* / Q000235:genetics; D009687:Nuclear Proteins; D016133:Polymerase Chain Reaction; D011379:Prognosis; D011518:Proto-Oncogene Proteins / Q000032:analysis* / Q000235:genetics; D012189:Retrospective Studies; D016019:Survival Analysis
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; D004273:DNA, Neoplasm; D004268:DNA-Binding Proteins; C501486:MLH1 protein, human; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D011518:Proto-Oncogene Proteins; C497172:MSH2 protein, human; D000070957:MutL Protein Homolog 1; D051718:MutS Homolog 2 Protein