[Reversion of the malignant phenotype of gastric cancer by c-erbB-2 specific ribozyme].
OBJECTIVE
To probe the effect of c-erbB-2 specific ribozyme to the malignant phenotype of gastric cancer.


METHODS
The eucaryotic expression vector of c-erbB-2 specific ribozyme RZ1 was designed by computer and named pDOR-RZ1. The transfection of the gastric cancer cell line SGC-7901 was mediated by Lipofect AMINE. Flow cytometry was used to analyse the expression of the c-erbB-2 product P185. In vivo study of tumorogenecity of the transfected cells was performed in nude mice.


RESULTS
pDOR-RZ1 was successfully transfected into the gastric cancer cell line SGC-7901 and then the single clones were selected by G418 and named SGC/RZ1. Flow cytometry analysis showed that the expression of P185 was suppressed by 62.7%. The growth rate of SGC/RZ1 was inhibited by 55%. The tumor-forming time in SGC/RZ1 in nude mice was delayed remarkably and the tumor size was also much smaller than that in the control group, indicating the inhibition of the tumorogenecity of SGC/RZ1 in nude mice.


CONCLUSIONS
c-erbB-2 specific ribozyme is very efficient in reversing the malignant phenotype of gastric cancer. This might provide a new approach for gene therapy of gastric cancer.
Bi
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F
Institute of Digestive Diseases of PLA, Fourth Military Medical University, Xi'an 710032.
Zhang
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X
Hui
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H
D000818:Animals; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D010641:Phenotype; D016337:RNA, Catalytic / Q000096:biosynthesis / Q000235:genetics; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000096:biosynthesis* / Q000235:genetics; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology*; D014162:Transfection; D014407:Tumor Cells, Cultured
D016337:RNA, Catalytic; D018719:Erb-b2 Receptor Tyrosine Kinases