title: Molecular cloning and characterization of reptilian estrogen receptor cDNAs.
abstract: cDNAs encoding alligator (caiman), Caiman crocodilus and whiptail lizard, Cnemidophorus uniparens estrogen receptors (ERs) were cloned and sequenced. This is the first report of full-length cDNA sequences for reptilian ERs, to our knowledge. The full-length alligator (caiman) ER cDNA (1764 bp, 587 amino acid residues) shows 68% amino acid homology with the full-length whiptail lizard ER cDNA (1746 bp, 581 amino acid residues). The respective ligand binding E domains have 87 and 83% amino acid homology with human ER while the DNA binding C domains show 100% amino acid homology with the human, rat and chicken forms. When the cDNAs were inserted into the pRc/RSV vector and transfected into HeLa cells with a reporter plasmid, the encoding proteins were confirmed to be functional through the interaction of the receptor-ligand complex with the estrogen responsive element (ERE).
authors.affiliation: Environmental Health Science Laboratory, Sumitomo Chemical Co., Ltd., 1-98, 3-Chome, Kasugade-Naka, Konohana-ku, Osaka 554-8558, Japan. sumida@sc.sumitomo-chem.co.jp
authors.forename: K
authors.initials: K
authors.lastname: Sumida
authors.forename: N
authors.initials: N
authors.lastname: Ooe
authors.forename: K
authors.initials: K
authors.lastname: Saito
authors.forename: H
authors.initials: H
authors.lastname: Kaneko
mesh_terms: D000489:Alligators and Crocodiles / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D017930:Genes, Reporter; D006367:HeLa Cells; D006801:Humans; D008116:Lizards / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D011960:Receptors, Estrogen / Q000737:chemistry / Q000235:genetics*; D030162:Reptilian Proteins / Q000737:chemistry / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection
chemical_list: D018076:DNA, Complementary; D011960:Receptors, Estrogen; D030162:Reptilian Proteins