Interaction of picornavirus 2C polypeptide with the viral negative-strand RNA.
The picornavirus membrane-associated polypeptide 2C is believed to be required for viral RNA synthesis. Hepatitis A virus (HAV)- and human rhinovirus (HRV)-encoded recombinant 2C proteins have been expressed, purified and examined for their ability to interact with the terminal sequences of viral positive- and negative-strand RNAs. The results demonstrate that both the HAV- and the HRV-encoded 2C polypeptide specifically interact with the 3'-terminal sequences of the negative-strand RNA, but not with the complementary sequences at the 5' terminus of the positive-strand RNA. This interaction was detected by both mobility gel shift and UV cross-linking assays. Furthermore, complex formation exhibited dose-dependency and competition assays confirmed specificity. These results are consistent with our previous observation using the poliovirus 2C protein. The implication of the picornavirus 2C protein binding to the 3'-terminal sequence of the negative-strand untranslated region in viral RNA synthesis is discussed.
Banerjee
Rajeev
R
Department of Microbiology, Immunology and Molecular Genetics, UCLA School of Medicine, University of California at Los Angeles, Los Angeles, California, CA-90095, USA1.
Dasgupta
Asim
A
Department of Microbiology, Immunology and Molecular Genetics, UCLA School of Medicine, University of California at Los Angeles, Los Angeles, California, CA-90095, USA1.
D020413:3' Untranslated Regions / Q000378:metabolism; D001483:Base Sequence; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017957:Hepatitis A Virus, Human / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012229:Rhinovirus / Q000235:genetics / Q000378:metabolism*; D017361:Viral Nonstructural Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
D020413:3' Untranslated Regions; D002352:Carrier Proteins; D012367:RNA, Viral; D011994:Recombinant Proteins; D017361:Viral Nonstructural Proteins; C076023:2C protein, viral