[Screening and characterization of human phage antibody to hepatitis C core antigen].
OBJECTIVE
To screen and characterize human phage antibody (ScFv) against hepatitis C core antigen.


METHODS
The recombinant phages were panned by core antigen that was coated in a microtiter plate. After three rounds of biopanning, 48 clones were determined specific to core antigen. The specificity of each ScFv colone was determined by ELISA. The coding gene for HCV protein ScFv has been sequenced.


RESULTS
Phage antibody for HCV core protein had a specific combination character with core antigen of hepatitis C virus. The DNA sequence data showed that the ScFv gene included 774 bp.


CONCLUSIONS
Human single chain antibody to hepatitis C core antigen has been identified by means of the phage display technology.
Zhong
Y
Y
Gene Therapy Research Center, Institute of Infectious Diseases, The 302nd Hospital of PLA, Beijing, 100039, China.
Cheng
J
J
Shi
S
S
Xia
X
X
Wang
G
G
Yang
J
J
Chen
J
J
D000595:Amino Acid Sequence; D000914:Antibodies, Viral / Q000276:immunology*; D000956:Antigens, Viral / Q000276:immunology*; D001483:Base Sequence; D001667:Binding, Competitive; D004279:DNA, Viral / Q000032:analysis; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000235:genetics / Q000276:immunology; D008969:Molecular Sequence Data; D019151:Peptide Library; D014758:Viral Core Proteins / Q000276:immunology*
D000914:Antibodies, Viral; D000956:Antigens, Viral; D004279:DNA, Viral; D007135:Immunoglobulin Variable Region; D019151:Peptide Library; D014758:Viral Core Proteins; C069130:nucleocapsid protein, Hepatitis C virus