[Inhibitory effect of methanol extract of Boschniakia rossica Fedtsch. et Flerov on rat hepatic preneoplastic lesions induced by diethylnitrosamine].
OBJECTIVE
To investigate the inhibitory effect of Boschniakia rossica(BR) on hepatocarcinogenesis in rats.


METHODS
Based on immunohistochemistry techniques, the expression of placental form glutathione S-transferase(GST-P), mutant p53 and p21 protein were investigated in hepatic preneoplastic lesions induced by Solt-Farber protocol in the liver of rats that had been treated with the above method, administered with BR extract and of control group.


RESULTS
The extract of BR(500 mg/kg) has inhibitory effect on the formation of diethylnitrosamine-induced GST-P-positive foci in F344 rat and the expression of mutant p53 and p21 protein was lower than that of hepatic preneoplastic lesions, and the increasing gamma-glutamyltranspeptidase(gamma-GT) activity in rat liver treated with Solt-Farber protocol was decreased by the extract of BR.


CONCLUSIONS
These results indicate that BR has inhibitory effect on DEN induced hepatic preneoplastic lesions in F344 rat.
Yin
Z Z
ZZ
Biochemistry Laboratory for Cancer Research, Yanbian University College of Medicine, Yanji 133000.
Jin
H L
HL
Li
T Z
TZ
Lee
J J
JJ
Kim
Y H
YH
Lee
C H
CH
Lee
K H
KH
Hyun
B H
BH
D000818:Animals; D050759:Cyclin-Dependent Kinase Inhibitor p21; D016213:Cyclins / Q000378:metabolism; D004052:Diethylnitrosamine; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D005982:Glutathione Transferase / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D019684:Magnoliopsida / Q000737:chemistry*; D008297:Male; D010946:Plants, Medicinal / Q000737:chemistry*; D011230:Precancerous Conditions / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D051381:Rats; D011916:Rats, Inbred F344; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
C493958:Cdkn1a protein, rat; D050759:Cyclin-Dependent Kinase Inhibitor p21; D016213:Cyclins; D004365:Drugs, Chinese Herbal; D016159:Tumor Suppressor Protein p53; D004052:Diethylnitrosamine; D005982:Glutathione Transferase