Effects of rhidosin on expression of fibroblast growth factor on olfactory bulb in rats and its relation with senile hyposmia.
OBJECTIVE
To determine the expression of fibroblast growth factor (FGF) on olfactory bulb in rats and its relation with senile hyposmia, and to discuss the effects of rhidosin on expression of FGF on olfactory bulb in senile rats and its significance.


METHODS
Four groups of rats, i.e., young group, senile group, rhidosin group, and physiological saline control group were used in this study. After the removal of the heads of these rats, olfactory mucosa and olfactory bulbs were immediately fixed with neutral formalin, followed with paraffin-embedding, serial sectioning, immunohistochemical staining and light microscopic observation.


RESULTS
The positive expression rate of FGF on olfactory bulb in young group was significantly higher than that in senile group (P < 0.01). The positive expression rate of FGF on olfactory bulb in rhidosin group was significantly higher than that in physiological saline control group (P < 0.01). No positive expression of FGF on olfactory mucosa was observed in these four groups.


CONCLUSIONS
These results suggest that the expression of FGF on olfactory bulb gradually decline with aging. The decrease of expression of FGF on olfactory bulb may play a key role in the occurrence of senile hyposmia. Rhidosin can increase the expression of FGF on olfactory bulbs in senile rats and this may be one of the mechanisms by which rhidosin promotes the growth of olfactory cells and inhibits aging effects on the olfactory system.
Wang
Y
Y
Department of Otorhinolaryngology & Head and Neck Surgery, Third Teaching Hospital, Bethune University of Medical Sciences, Changchun 130031, China. wangyipe@public.cc.jl.cn
Guan
G
G
Dong
Z
Z
Yang
Z
Z
D000367:Age Factors; D000375:Aging; D000818:Animals; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005346:Fibroblast Growth Factors / Q000096:biosynthesis*; D008297:Male; D000857:Olfaction Disorders / Q000503:physiopathology*; D009830:Olfactory Bulb / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D004365:Drugs, Chinese Herbal; D005346:Fibroblast Growth Factors