title: Effect of lithium on growth of bone marrow stromal cells.
abstract: OBJECTIVE
  To investigate the effect of lithium on the growth of bone marrow stromal cells.
  
  
  METHODS
  We compared the different effects of lithium with the concentration of 0.1-12 mmol/L on the growth of murine fibroblast colony forming unit (mCFU-F), human fibroblast colony forming unit (hCFU-F) and their intercolonial cells. Pure murine bone marrow stromal cell assay was used to investigate the mechanism.
  
  
  RESULTS
  Cultures with 1-4 mmol/L lithium resulted in significant increase in the number of hCFU-F, but distinct decrease in the number of mCFU-F. Furthermore, there were much more endothelial cells between mCFU-Fs than those between hCFU-Fs. The direct effect of 1-4 mmol/L lithium on the growth of pure murine fibroblasts and endothelial cells was stimulatory. Serum-free endothelial cell conditioned medium (EC-CM) could inhibit the growth of mCFU-F.
  
  
  CONCLUSIONS
  1-4 mmol/L lithium has stimulatory effect on the growth of pure murine fibroblasts and endothelial cells. The inhibitory effect of lithium on mCFU-F formation may result from the effect of increased endothelial cells.
authors.affiliation: Research Laboratory of Blood Physiology, Hunan Medical University, Changsha, Hunan 410078, China.
authors.forename: W
authors.initials: W
authors.lastname: Huang
authors.forename: D
authors.initials: D
authors.lastname: Jiang
authors.forename: B
authors.initials: B
authors.lastname: Wang
authors.forename: Q
authors.initials: Q
authors.lastname: Wang
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology; D006412:Hematopoietic Stem Cells / Q000187:drug effects; D006801:Humans; D008094:Lithium / Q000494:pharmacology*; D051379:Mice; D013234:Stem Cells / Q000187:drug effects; D017154:Stromal Cells / Q000166:cytology
chemical_list: D008094:Lithium