Simultaneous determination of six triazolic pesticide residues in apple and pear pulps by liquid chromatography with ultraviolet diode array detection.
A method is described for the simultaneous determination of diclobutrazol, flusilazole, flutriafol, hexaconazole, paclobutrazol, and tetraconazole in apple and pear pulps used in baby food at a limit of 0.01 mg/kg. Apple and pear pulp samples are subjected to selective solid-phase microdispersion (SPMD) with SPE-ED Matrix-38 and acetone-cyclohexane, and the extracts are cleaned up on a Florisil cartridge with hexane-cyclohexane-acetone. The extracts are then analyzed by liquid chromatography with ultraviolet detection, using an octadecylsilane column with a gradient-programmed acetonitrile-water mobile phase. Recoveries were determined by spiking apple and pear pulps with the 6 pesticides under investigation at 0.1, 0.05, 0.03, and 0.01 mg/kg. Six determinations were performed at each level for each pesticide. Recoveries were > or = 70% at the 0.01 mg/kg level.
Bicchi
C
C
Università Degli Studi di Torino, Dipartimento di Scienza e Tecnologia del Farmaco, Italy.
Cordero
C
C
Rubiolo
P
P
Occelli
A
A
D002138:Calibration; D002853:Chromatography, Liquid; D005638:Fruit / Q000737:chemistry*; D006801:Humans; D007202:Indicators and Reagents; D007223:Infant; D007225:Infant Food / Q000032:analysis*; D027845:Malus / Q000737:chemistry*; D010573:Pesticide Residues / Q000032:analysis*; D012015:Reference Standards; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet; D014230:Triazoles / Q000032:analysis*
D007202:Indicators and Reagents; D010573:Pesticide Residues; D012997:Solvents; D014230:Triazoles