[Reduction of mitochondrial transmembrane potential constitutes an early event of peripheral blood monocytes apoptosis in hemodialysis patients].
OBJECTIVE
To investigate the relationship between monocyte apoptosis and mitochondria function in hemodialysis (HD) patients.


METHODS
Fluorescein labeled Annexin-V was used to detect the phosphatidyserine (PS) of apoptosis cells, and mitochondrial transmembrane potential was studied by 3,3'-dihexyloxacarbocyanine iodide, Di0C6(3), and analyzed by flow cytometry. Swelling of mitochondrial permeability transition (PT) was recorded by spectrophotometer.


RESULTS
Spontaneous apoptosis of cultured monocytes increased at 24, 72 hours in HD patients (41.4 +/- 12.2)%, (22.0 +/- 3.0)% as compared with controls (20.6 +/- 8.8)%, (9.8 +/- 2.1)%. Freshly isolated monocytes in HD patients had little apoptosis, but mitochondrial transmembrane potential was reduced to half of disruption (43 +/- 9)%. As to swelling of PT, those freshly isolated monocytes mitochondria in HD patients experienced overwhelming opening of PT, (80 +/- 5.6)%.


CONCLUSIONS
Swelling of PT and breakdown of mitochondrial transmembrane potential constitute the early events of monocytes apoptosis in HD patients, which could contribute to the impaired monocytes function.
Jin
H M
HM
Department of Nephrology, Shanghai Medical University, Shanghai First People's Hospital, Shanghai 200080.
Xu
Q J
QJ
Wu
Z J
ZJ
D000328:Adult; D017209:Apoptosis; D002478:Cells, Cultured; D005260:Female; D005921:Glomerulonephritis / Q000097:blood / Q000628:therapy*; D006801:Humans; D008297:Male; D008564:Membrane Potentials; D008928:Mitochondria / Q000378:metabolism / Q000473:pathology*; D008933:Mitochondrial Swelling; D009000:Monocytes / Q000473:pathology*; D006435:Renal Dialysis