Signalling of GPI-anchored CD157 via focal adhesion kinase in MCA102 fibroblasts.
CD157, a glycosylphosphatidylinositol-anchored protein, has previously been shown to mediate tyrosine phosphorylation of a 130 kDa protein (p130) in several cell lines. In this study, we have identified the p130 protein to be focal adhesion kinase (FAK or pp125(FAK)). FAK undergoes phosphorylation at Tyr-397 and Tyr-861 in intact MCA102 cells stably transfected with CD157 (MCA/CD157). MCA/CD157 cells, which displayed a rounded and compact cell morphology, exhibited a dispersed distribution, in contrast to a more closely associated and elongated spindle cell shape in the vector-transfected cells. MCA/CD157 cells proliferated at a rate 20-25% slower than the control cells. Our results demonstrate, for the first time, that FAK is a downstream signalling molecule of CD157.
Liang
F
F
Department of Biochemistry, Faculty of medicine, National University of Singapore, 119260, Singapore.
Qi
R Z
RZ
Chang
C F
CF
D036541:ADP-ribosyl Cyclase; D015703:Antigens, CD; D015153:Blotting, Western; D002460:Cell Line; D005347:Fibroblasts / Q000201:enzymology / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D051416:Focal Adhesion Protein-Tyrosine Kinases; D058851:GPI-Linked Proteins; D017261:Glycosylphosphatidylinositols / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000378:metabolism*; D011233:Precipitin Tests; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D015398:Signal Transduction
D015703:Antigens, CD; D058851:GPI-Linked Proteins; D017261:Glycosylphosphatidylinositols; D008562:Membrane Glycoproteins; D011505:Protein-Tyrosine Kinases; D051416:Focal Adhesion Protein-Tyrosine Kinases; D036541:ADP-ribosyl Cyclase; C087521:ADP-ribosyl cyclase 2