Rapid expression of vascular endothelial growth factor in early acute myocardial ischemia in rats.
OBJECTIVE
To study the expression of vascular endothelial growth factor (VEGF) in different parts of heart during early acute myocardial ischemia.


METHODS
The expression of VEGF in control and study group in rats was performed with the Strept Avidin-Biotin-Peroxidase Complex (SABC) method.


RESULTS
A rapid expression of VEGF in ischemic areas was observed 1 hour after the ligation of rat left anterior descending (LAD) coronary artery, and in peri-ischemic and non-ischemic areas 3 hours after ischemia. The grade of expression of VEGF increased with the continuation of myocardial ischemia, the positive myocytes of SABC-VEGF staining mainly localized in peri-ischemic areas 5 hours after ischemia. No expression of VEGF in control group was found.


CONCLUSIONS
The expression of VEGF may be up-regulated in rapid and continuous manner with characteristic spatial and temporal kinetic during early acute myocardial ischemia.
Chen
J
J
Department of Pathology, ShanTou University Medical College, Shantou 515031, China.
Xu
X
X
Wu
X
X
Zhu
J
J
D000818:Animals; D016228:Endothelial Growth Factors / Q000096:biosynthesis*; D005260:Female; D008222:Lymphokines / Q000096:biosynthesis*; D008297:Male; D017202:Myocardial Ischemia / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
D016228:Endothelial Growth Factors; D008222:Lymphokines; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors