title: Novel tetracycline resistance gene, tet(32), in the Clostridium-related human colonic anaerobe K10 and its transmission in vitro to the rumen anaerobe Butyrivibrio fibrisolvens.
abstract: A novel tetracycline resistance gene, designated tet(32), which confers a high level of tetracycline resistance, was identified in the Clostridium-related human colonic anaerobe K10, which also carries tet(W). tet(32) was transmissible in vitro to the rumen anaerobe Butyrivibrio fibrisolvens 2221(R). The predicted gene product of tet(32) has 76% amino acid identity with Tet(O). PCR amplification indicated that tet(32) is widely distributed in the ovine rumen and in porcine feces.
authors.affiliation: Rowett Research Institute, Bucksburn, Aberdeen, United Kingdom AB21 9SB.
authors.forename: C M
authors.initials: CM
authors.lastname: Melville
authors.forename: K P
authors.initials: KP
authors.lastname: Scott
authors.forename: D K
authors.initials: DK
authors.lastname: Mercer
authors.forename: H J
authors.initials: HJ
authors.lastname: Flint
mesh_terms: D000693:Anaerobiosis; D000818:Animals; D002417:Cattle; D003013:Clostridium / Q000187:drug effects / Q000235:genetics*; D003106:Colon / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012417:Rumen / Q000382:microbiology*; D013753:Tetracycline Resistance / Q000235:genetics*; D014737:Vibrionaceae / Q000187:drug effects*
chemical_list: D004269:DNA, Bacterial