Coping with kinetic and thermodynamic barriers: RMCE, an efficient strategy for the targeted integration of transgenes.
Site-specific recombinases have become powerful tools for the targeted integration of transgenes into defined chromosomal loci. They have been successfully used both to achieve predictable gene expression in cell culture and for the systematic creation of transgenic animals. A recent improvement of this method, the recombinase-mediated cassette exchange procedure (RMCE), permits expression in the absence of any co-expressed selection marker gene.
Baer
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Gesellschaft für Biotechnologische Forschung mbH (GBF), German Research Institute for Biotechnology, RDIF/Epigenetic Regulation, D-38124 Braunschweig, FRG, Mascheroder Weg 1, Braunschweig, Germany. aba@gbf.de
Bode
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D000818:Animals; D004254:DNA Nucleotidyltransferases / Q000235:genetics* / Q000502:physiology; D018390:Gene Targeting / Q000379:methods*; D019426:Integrases / Q000235:genetics / Q000502:physiology; D007700:Kinetics; D016254:Mutagenesis, Insertional / Q000379:methods*; D016297:Mutagenesis, Site-Directed; D011401:Promoter Regions, Genetic; D011995:Recombination, Genetic; D013816:Thermodynamics; D019076:Transgenes; D014764:Viral Proteins / Q000235:genetics / Q000502:physiology
D014764:Viral Proteins; C045073:Cre recombinase; D004254:DNA Nucleotidyltransferases; C045119:FLP recombinase; D019426:Integrases