Influence of steroids on microvascular perfusion injury of the bowel induced by extracorporeal circulation.
BACKGROUND
Extracorporeal circulation is associated with gastrointestinal complications. By means of intravital microscopic methods, we investigated whether preoperative treatment with steroids can attenuate the impairment of the bowel microcirculation.


METHODS
In 20 pigs, a partial left heart bypass (pLHB) was established. A loop of the terminal ileum was exteriorized for intravital-microscopic observation. Seven sham-operated animals served as controls. In 13 animals, pLHB was established for 2 hours with a flow rate of 2,000 mL per minute; 7 of the animals received 20 mg/kg body weight prednisolone preoperatively. The microcirculatory network was analyzed before, during pLHB, and 2 hours after bypass.


RESULTS
Despite unchanged macro-hemodynamics, pLHB resulted in a significant microvascular perfusion injury of the small bowel. Arteriolar vasoconstriction and a reduction of perfused capillaries per unit area (functional capillary density) to 30% of prebypass values could be found. Blood cell velocities were reduced in submucuous collecting venules. In the steroid-treated animals, the functional capillary density remained normal. In addition, arteriolar vasoconstriction could be prevented.


CONCLUSIONS
Treatment with prednisolone largely prevents the microcirculatory alterations in the small bowel induced by extracorporeal circulation.
Sack
F U
FU
Department of Cardiac Surgery, University of Heidelberg, Germany. falk-udo.sack@urz.uni-heidelberg.de
Reidenbach
B
B
Dollner
R
R
Schledt
A
A
Gebhard
M M
MM
Hagl
S
S
D000818:Animals; D001783:Blood Flow Velocity / Q000187:drug effects; D005112:Extracorporeal Circulation; D007082:Ileum / Q000098:blood supply / Q000473:pathology; D007422:Intestines / Q000098:blood supply* / Q000473:pathology; D008833:Microcirculation / Q000187:drug effects / Q000473:pathology; D011239:Prednisolone / Q000031:analogs & derivatives* / Q000494:pharmacology*; D011292:Premedication; D012039:Regional Blood Flow / Q000187:drug effects; D015427:Reperfusion Injury / Q000473:pathology*; D013552:Swine
D011239:Prednisolone; C021322:prednisolone hemisuccinate