[Determination of content of radix Paeoniae Rubar(Chishao)801 in rabbit's plasma by HPLC].
A HPLC method was used to determine the content of Radix Paeoniae Rubar (Chishao)801 in rabbit's plasma, using Nucleosil C18 column, methanol, water and glacial acetic acid (48:50:2) as mobile phase ethye 4-hydroxybenzoate as internal standard, and UV-detector(lambda max: 275 nm, AUFS: 0.02). The average recovery was 96.70%(C: 2.5 micrograms/ml) and 98.86%(C: 5 micrograms/ml) respectively. The lowest determination limit was 0.1 microgram/ml. The method is simple, accurate, sensitive and reliable.
Wang
Y
Y
Institute of Chinese Materia Medica, China Academy of Traditional Chinese Medicine, Beijing 100700.
Li
J
J
Du
G
G
Wang
X
X
Cui
H
H
Zhao
Y
Y
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D008297:Male; D011435:Propyl Gallate / Q000097:blood*; D011817:Rabbits
D004365:Drugs, Chinese Herbal; D011435:Propyl Gallate