Integrated enzyme-linked immunosorbent assay screening system for amnesic, neurotoxic, diarrhetic, and paralytic shellfish poisoning toxins found in New Zealand.
Enzyme-linked immunosorbent assays (ELISAs) were developed for amnesic, neurotoxic, and diarrhetic shellfish poisoning (ASP, NSP, and DSP) toxins and for yessotoxin. These assays, along with a commercially available paralytic shellfish poisoning (PSP) ELISA, were used to test the feasibility of an ELISA-based screening system. It was concluded that such a system to identify suspect shellfish samples, for subsequent analysis by methods approved by international regulatory authorities, is feasible. The assays had sufficient sensitivity and can be used on simple shellfish extracts. Alcohol extraction gave good recovery of all toxin groups. The ease of ELISAs permits the ready expansion of the system to screen for other toxins, as new ELISAs become available.
Garthwaite
I
I
AgResearch Ruakura, Toxinology and Food Safety Group, Hamilton, New Zealand.
Ross
K M
KM
Miles
C O
CO
Briggs
L R
LR
Towers
N R
NR
Borrell
T
T
Busby
P
P
D000647:Amnesia / Q000139:chemically induced*; D000818:Animals; D000918:Antibody Specificity; D003967:Diarrhea / Q000139:chemically induced*; D004797:Enzyme-Linked Immunosorbent Assay; D004988:Ethers, Cyclic / Q000032:analysis; D008387:Marine Toxins / Q000032:analysis* / Q000633:toxicity; D008978:Mollusk Venoms / Q000032:analysis; D009498:Neurotoxins / Q000032:analysis* / Q000633:toxicity; D009520:New Zealand; D035964:Oxocins; D010243:Paralysis / Q000139:chemically induced*; D011933:Reagent Kits, Diagnostic; D012758:Shellfish / Q000032:analysis*; D012997:Solvents
D004988:Ethers, Cyclic; D008387:Marine Toxins; D008978:Mollusk Venoms; D009498:Neurotoxins; D035964:Oxocins; D011933:Reagent Kits, Diagnostic; D012997:Solvents; C066632:yessotoxin