Cloning of the rat IL-5Ralpha gene: analysis of 5'-upstream region and expression by B cells.
Although rats are widely used for the analysis of allergic reactions and parasitic infections where IL-5 is involved, nothing is currently known of the expression of IL-5 receptor in this species. In this study, the cDNA sequence, genomic structure and the transcriptional regulation of the rat IL-5Ralpha were analyzed. The rat IL-5Ralpha gene, which we localized to chromosome 4q34-q41, spans more than 25 kb and consists of 12 exons. Promoter activity was seen in different cell lines and analysis by deletion experiments allowed to identify two negative regulatory regions which did not differ when tested either with IL-5Ralpha-negative or positive cells. Finally, the investigation of the expression of IL-5Ralpha showed that it is expressed in lung, spleen, liver, and purified rat B cells from normal rat. This can provide an explanation for the role of rat IL-5 as B-cell growth factor and a relevant model in order to better understand the activity of IL-5 on human B cells.
Pierrot
C
C
Unité INSERM 547, IFR 17, CNRS 1160, Institut Pasteur de Lille, Institut de Biologie de Lille, 1 rue du Prof. Calmette, 59019 Lille, France.
Bègue
A
A
Szpirer
C
C
Capron
A
A
Capron
M
M
Khalife
J
J
D020121:5' Untranslated Regions / Q000032:analysis*; D000595:Amino Acid Sequence; D000818:Animals; D001402:B-Lymphocytes / Q000502:physiology*; D001483:Base Sequence; D002874:Chromosome Mapping; D002875:Chromosomes; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis; D018628:Gene Dosage; D015870:Gene Expression; D016678:Genome; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics*; D051381:Rats; D011916:Rats, Inbred F344; D018123:Receptors, Interleukin / Q000096:biosynthesis / Q000235:genetics*; D053649:Receptors, Interleukin-5; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic
D020121:5' Untranslated Regions; D018123:Receptors, Interleukin; D053649:Receptors, Interleukin-5; D004247:DNA