title: Protective effect of gypenosides on DNA and RNA of rat neurons in cerebral ischemia-reperfusion injury.
abstract: OBJECTIVE
  To observe the protective effect of gypenosides (GP) on the neurons of hippocampus, cerebral cortex, corpus striatum, and dentate gyrus in cerebral ischemia-reperfusion injury of rats.
  
  
  METHODS
  Modified 4-vessel occlusion (4-VO) method was used to establish the model of acute global ischemia. The acridine orange (AO) staining method was used to observe the DNA and RNA contents of cerebral ischemia-reperfusion injury model in the areas.
  
  
  RESULTS
  The fluorescent intensity (reflecting DNA and RNA contents) of the DNA and RNA in the areas of cerebral ischemia-reperfusion injury was markedly abated compared with the normal control group. In the group of ig GP (100 mg/kg) it was enhanced compared with the model group and was the same as the normal control group.
  
  
  CONCLUSIONS
  The injury of the DNA and RNA in the areas of ischemia-reperfusion model was decreased by GP.
authors.affiliation: Department of Pharmacology, Medical College of CPAPF, Tianjin 300162, China.
authors.forename: G
authors.initials: G
authors.lastname: Qi
authors.forename: L
authors.initials: L
authors.lastname: Zhang
authors.forename: W L
authors.initials: WL
authors.lastname: Xie
authors.forename: X Y
authors.initials: XY
authors.lastname: Chen
authors.forename: J S
authors.initials: JS
authors.lastname: Li
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D002545:Brain Ischemia / Q000150:complications; D002540:Cerebral Cortex / Q000378:metabolism; D019662:Cucurbitaceae / Q000737:chemistry*; D004247:DNA / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D008297:Male; D009474:Neurons / Q000378:metabolism; D018696:Neuroprotective Agents / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry; D012313:RNA / Q000378:metabolism*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000209:etiology / Q000378:metabolism*; D012503:Saponins / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D004365:Drugs, Chinese Herbal; D018696:Neuroprotective Agents; D012503:Saponins; D012313:RNA; C077955:gypenoside IX; D004247:DNA