title: Very low and intermediate density lipoprotein fractions from apolipoprotein E gene-knockout mice induce cholesteryl ester accumulation in J774 macrophages.
abstract: OBJECTIVE
  To investigate the atherogenic role of very low density lipoprotein (VLDL) and intermediate density lipoprotein (IDL) fraction isolated from plasma of apolipoprotein E (apoE)-knockout mice.
  
  
  METHODS
  Containing both VLDL and IDL (apoEko-VLDL/IDL), lipoprotein fraction was isolated from plasma of apoE-knockout mice by ultracentrifugation, and its interaction with J774 macrophages was examined together with its physicochemical properties including lipid content, electrophoresis mobility and apolipoprotein components.
  
  
  RESULTS
  ApoEko-VLDL/IDL could be actively taken up by J774 macrophages, resulting in marked cholesterol ester accumulation in the cells (55 +/- 9 nmol/mg cell protein), which was a 7-fold increase over the corresponding lever induced by low density lipoprotein and significantly higher than non-loaded group (P < 0.01).
  
  
  CONCLUSIONS
  ApoEko-VLDL/IDL induced significant cholesterol ester accumulation through an apoE independent pathway. This pathway may explain, partly the mechanism of cholesterol deposition in arterial wall in apoE-knockout mice.
authors.affiliation: Department of Medical Laboratory Science, Nanjing General Hospital of PLA, Nanjing 210002, China.
authors.forename: C
authors.initials: C
authors.lastname: Zhang
authors.forename: M
authors.initials: M
authors.lastname: Akira
authors.forename: H
authors.initials: H
authors.lastname: Hideki
authors.forename: S
authors.initials: S
authors.lastname: Hisashi
authors.forename: H
authors.initials: H
authors.lastname: Seikoh
mesh_terms: D000818:Animals; D001057:Apolipoproteins E / Q000378:metabolism*; D015815:Cell Adhesion Molecules / Q000378:metabolism; D002788:Cholesterol Esters / Q000378:metabolism*; D006801:Humans; D008074:Lipoproteins / Q000378:metabolism*; D053419:Lipoproteins, IDL; D008079:Lipoproteins, VLDL / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D018345:Mice, Knockout; D011971:Receptors, Immunologic / Q000378:metabolism; D051116:Receptors, Scavenger
chemical_list: D001057:Apolipoproteins E; D015815:Cell Adhesion Molecules; D002788:Cholesterol Esters; D008074:Lipoproteins; D053419:Lipoproteins, IDL; D008079:Lipoproteins, VLDL; D011971:Receptors, Immunologic; D051116:Receptors, Scavenger