title: [Transfection of Fas(CD95) gene into HL-60 cell and its role in rhG-CSF-induced apoptosis].
abstract: OBJECTIVE
  To explore the role of Fas gene in rhG-CSF-induced apoptosis.
  
  
  METHODS
  Mediated by lipofection (DOTAP), Fas cDNA was transferred into HL-60 cells. In situ hybridization, Western blotting and FCM analysis were used to demonstrate the successful transfection. The apoptosis percentages of HL-60 and Fas transfected HL-60 cells were compared after coculturing with rhG-CSF at the same final concentration.
  
  
  RESULTS
  After cocultured with rhG-CSF (10 ng/ml) for 96 hours, the apoptosis percentage of Fas transferred HL-60 cells was significantly higher than that of HL-60 cells, and the Fas expressions could be up-regulated by rhG-CSF.
  
  
  CONCLUSIONS
  Fas is an apoptosis-induction oncogene, and rhG-CSF induces apoptosis of HL-60 cells through Fas signalling pathway.
authors.affiliation: Shandong Provincial Hospital, Jinan 250021.
authors.forename: Y
authors.initials: Y
authors.lastname: Song
authors.forename: G
authors.initials: G
authors.lastname: Xu
authors.forename: L
authors.initials: L
authors.lastname: Zhang
mesh_terms: D017209:Apoptosis; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D018922:HL-60 Cells / Q000473:pathology*; D006801:Humans; D011994:Recombinant Proteins; D014162:Transfection; D019014:fas Receptor / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D011994:Recombinant Proteins; D019014:fas Receptor; D016179:Granulocyte Colony-Stimulating Factor