title: Genome organization of the densovirus from Bombyx mori (BmDNV-1) and enzyme activity of its capsid.
abstract: Bombyx mori densovirus (BmDNV-1), on the basis of the previously reported genome sequence, constitutes by itself a separate genus (Iteravirus) within the Densovirinae subfamily of parvoviruses. Inconsistencies in the genome organization, however, necessitated its reassessment. The genome sequence of new clones was determined and resulted in a completely different genome organization. The corrected sequence also contained conserved sequence motifs found in other parvoviruses. Some amino acids in the highly conserved domain in the unique region of VP1 were shared by critical amino acids in the catalytic site and Ca(2+)-binding loop of secreted phospholipase A2, such as from snake and bee venoms. Expression of this domain and determination of enzyme activity demonstrated that capsids have a phospholipase A2 activity thus far unknown to occur in viruses. This viral phospholipase A2, which is required shortly after entry into the cell, showed a substrate preference for phosphatidylethanolamine and phosphatidylcholine over phosphatidylinositol.
authors.affiliation: Centre de recherche de microbiologie et biotechnologie, INRS-Institut Armand-Frappier, Université du Québec, 531 boul. des Prairies, Laval, QC, CanadaH7V 1B71.
authors.forename: Y
authors.initials: Y
authors.lastname: Li
authors.affiliation: Centre de recherche de microbiologie et biotechnologie, INRS-Institut Armand-Frappier, Université du Québec, 531 boul. des Prairies, Laval, QC, CanadaH7V 1B71.
authors.forename: Z
authors.initials: Z
authors.lastname: Zádori
authors.affiliation: Department of Bioscience, Faculty of Agriculture, Hokkaido University, Sapporo, Japan2.
authors.forename: H
authors.initials: H
authors.lastname: Bando
authors.affiliation: Centre de recherche de microbiologie et biotechnologie, INRS-Institut Armand-Frappier, Université du Québec, 531 boul. des Prairies, Laval, QC, CanadaH7V 1B71.
authors.forename: R
authors.initials: R
authors.lastname: Dubuc
authors.affiliation: Center of Virology-IRD, Faculty of Agriculture, University of Cairo, Giza, Cairo, Egypt3.|Centre de recherche de microbiologie et biotechnologie, INRS-Institut Armand-Frappier, Université du Québec, 531 boul. des Prairies, Laval, QC, CanadaH7V 1B71.
authors.forename: G
authors.initials: G
authors.lastname: Fédière
authors.affiliation: Centre de recherche de microbiologie et biotechnologie, INRS-Institut Armand-Frappier, Université du Québec, 531 boul. des Prairies, Laval, QC, CanadaH7V 1B71.
authors.forename: J
authors.initials: J
authors.lastname: Szelei
authors.affiliation: Centre de recherche de microbiologie et biotechnologie, INRS-Institut Armand-Frappier, Université du Québec, 531 boul. des Prairies, Laval, QC, CanadaH7V 1B71.
authors.forename: P
authors.initials: P
authors.lastname: Tijssen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D012831:Bombyx / Q000821:virology*; D002213:Capsid / Q000378:metabolism*; D017990:Densovirus / Q000201:enzymology* / Q000235:genetics; D016679:Genome, Viral; D008969:Molecular Sequence Data; D010741:Phospholipases A / Q000737:chemistry / Q000378:metabolism*; D054467:Phospholipases A2; D017422:Sequence Analysis, DNA
chemical_list: D010741:Phospholipases A; D054467:Phospholipases A2