Protective effect of gypenosides on DNA and RNA of rat neurons in cerebral ischemia-reperfusion injury.
OBJECTIVE
To observe the protective effect of gypenosides (GP) on the neurons of hippocampus, cerebral cortex, corpus striatum, and dentate gyrus in cerebral ischemia-reperfusion injury of rats.


METHODS
Modified 4-vessel occlusion (4-VO) method was used to establish the model of acute global ischemia. The acridine orange (AO) staining method was used to observe the DNA and RNA contents of cerebral ischemia-reperfusion injury model in the areas.


RESULTS
The fluorescent intensity (reflecting DNA and RNA contents) of the DNA and RNA in the areas of cerebral ischemia-reperfusion injury was markedly abated compared with the normal control group. In the group of ig GP (100 mg/kg) it was enhanced compared with the model group and was the same as the normal control group.


CONCLUSIONS
The injury of the DNA and RNA in the areas of ischemia-reperfusion model was decreased by GP.
Qi
G
G
Department of Pharmacology, Medical College of CPAPF, Tianjin 300162, China.
Zhang
L
L
Xie
W L
WL
Chen
X Y
XY
Li
J S
JS
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002545:Brain Ischemia / Q000150:complications; D002540:Cerebral Cortex / Q000378:metabolism; D019662:Cucurbitaceae / Q000737:chemistry*; D004247:DNA / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D008297:Male; D009474:Neurons / Q000378:metabolism; D018696:Neuroprotective Agents / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry; D012313:RNA / Q000378:metabolism*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000209:etiology / Q000378:metabolism*; D012503:Saponins / Q000302:isolation & purification / Q000494:pharmacology*
D004365:Drugs, Chinese Herbal; D018696:Neuroprotective Agents; D012503:Saponins; D012313:RNA; C077955:gypenoside IX; D004247:DNA