title: Interaction between cyclooxygenase-2 and inducible nitric oxide synthase in gastric cancer.
abstract: BACKGROUND
  Chronic expression of cylcooygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) may play an important role in gastric carcinogenesis. Thus, the purpose of our study was to assess the expression of COX-2 and iNOS messenger RNA (mRNA) in gastric cancer and to investigate the correlation between the expression of COX-2 and iNOS mRNA in these patients.
  
  
  METHODS
  Twenty-three gastric carcinoma specimens and accompanying adjacent specimens were obtained from surgical resection. The expression of COX-2 and iNOS were examined by comparative reverse transcription polymerase chain reaction.
  
  
  RESULTS
  Cylcooygenase-2 and iNOS mRNA were significantly higher in gastric cancer tissues than in adjacent normal tissues. There was significant correlation between the levels of COX-2 and iNOS mRNA in carcinoma tissues. However, there was no significant correlation between the level of COX-2 or iNOS mRNA expression and several clinicopathologic parameters in these patients.
  
  
  CONCLUSIONS
  The expression of COX-2 and iNOS may be one of the factors that contribute to gastric carcinogenesis.
authors.affiliation: Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea. hjson@smc.samsung.co.kr
authors.forename: H J
authors.initials: HJ
authors.lastname: Son
authors.forename: Y H
authors.initials: YH
authors.lastname: Kim
authors.forename: D I
authors.initials: DI
authors.lastname: Park
authors.forename: J J
authors.initials: JJ
authors.lastname: Kim
authors.forename: P L
authors.initials: PL
authors.lastname: Rhee
authors.forename: S W
authors.initials: SW
authors.lastname: Paik
authors.forename: K W
authors.initials: KW
authors.lastname: Choi
authors.forename: S Y
authors.initials: SY
authors.lastname: Song
authors.forename: J C
authors.initials: JC
authors.lastname: Rhee
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism*; D051546:Cyclooxygenase 2; D005260:Female; D006801:Humans; D007527:Isoenzymes / Q000096:biosynthesis* / Q000378:metabolism; D008297:Male; D008565:Membrane Proteins; D008875:Middle Aged; D019001:Nitric Oxide Synthase / Q000096:biosynthesis* / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II; D010544:Peroxidases / Q000096:biosynthesis* / Q000378:metabolism; D011451:Prostaglandin-Endoperoxide Synthases / Q000096:biosynthesis* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000378:metabolism*
chemical_list: D007527:Isoenzymes; D008565:Membrane Proteins; D012333:RNA, Messenger; D010544:Peroxidases; C496319:NOS2 protein, human; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; D011451:Prostaglandin-Endoperoxide Synthases