title: [Biological properties of reconstructed mouse hematopoiesis with hematopoietic stem cells transfected by bcl-2 gene].
abstract: OBJECTIVE
  To observe resistance to radiochemical agents in the mice reconstructed hematopoiesis with hematopoietic stem cells transfected by bcl-2 gene.
  
  
  METHODS
  The plasmid containing bcl-2 cDNA retrovirus vector was transfected to PA317 cells with Lipofecaine kite. A PA317 cell clone(D3) with high virus titer was obtained through G418 selection. The virus supernatant of D3 clone cells was used to transfect mouse embryo-liver hematopoietic stem cells. Hematopoiesis in the mice treated with lethal dose irradiation was reconstructed by hematopoietic stem cells transfected with bcl-2 gene. The resistance to radiochemical agents in the mice with reconstructed hematopoiesis was evaluated according to the number of WBC of periphereal blood and mononuclear cells of bone marrow, CFU-S and CFU-S regeneration.
  
  
  RESULTS
  The expression of bcl-2 protein was increased in the hematopoietic cells transfected by bcl-2 gene. The mice reconstructed hematopoiesis by stem cells transfected bcl-2 gene can tolerate high dose combined with chemotherapy and radiation of middle dose. The mice reconstructed hematopoiesis with stem cells transfected bcl-2 gene survived over one year, no tumors were found.
  
  
  CONCLUSIONS
  The mice with reconstructed hematopoiesis by stem cells carried bcl-2 gene possess the ability of resistance to radiochemical agents, even only part of hematopoietic stem cells were transfected with bcl-2 gene.
authors.affiliation: Institute of Hematology, State Key Lab. of Experimental Hematology, CAMS, PUMC, Tianjin 300020.
authors.forename: J
authors.initials: J
authors.lastname: Zhao
authors.forename: J
authors.initials: J
authors.lastname: Chu
authors.forename: S
authors.initials: S
authors.lastname: Ding
mesh_terms: D000818:Animals; D005333:Fetus; D019254:Genes, bcl-2 / Q000235:genetics*; D006410:Hematopoiesis / Q000502:physiology*; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D008099:Liver / Q000166:cytology; D051379:Mice; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis* / Q000235:genetics; D011836:Radiation Tolerance; D014162:Transfection; D014916:Whole-Body Irradiation
chemical_list: D019253:Proto-Oncogene Proteins c-bcl-2