Two novel cucurbitacins, neocucurbitacins A and B, from the Brazilian folk medicine "Buchinha" (Luffa operculata) and their effect on PEBP2alphaA and OCIF gene expression in a human osteoblast-like Saos-2 cell line.
Two novel cucurbitacins designated as neocucurbitacins A (1), possessing inhibitory activity of polyoma enhancer binding protein 2alphaA (PEBP2alphaA) and osteoclastogenesis-inhibitory factor (OCIF) gene expression in human osteoblast-like cells, and B (2) were isolated from the fruit of Luffa operculata. Their structures have been determined by extensive spectroscopic investigation.
Kawahara
N
N
National Institute of Health Sciences, Tokyo, Japan. kawahara@nihs.go.jp
Kurata
A
A
Hakamatsuka
T
T
Sekita
S
S
Satake
M
M
D002460:Cell Line; D050676:Core Binding Factor Alpha 2 Subunit; D019662:Cucurbitaceae / Q000737:chemistry*; D004268:DNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics*; D006023:Glycoproteins / Q000096:biosynthesis* / Q000235:genetics*; D006801:Humans; D029776:Luffa / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D010006:Osteoblasts / Q000378:metabolism*; D053244:Osteoprotegerin; D010936:Plant Extracts / Q000032:analysis; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D018160:Receptors, Cytoplasmic and Nuclear / Q000096:biosynthesis* / Q000235:genetics*; D018124:Receptors, Tumor Necrosis Factor; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013055:Spectrophotometry, Infrared; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics*
D050676:Core Binding Factor Alpha 2 Subunit; D004268:DNA-Binding Proteins; D006023:Glycoproteins; D053244:Osteoprotegerin; D010936:Plant Extracts; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D018160:Receptors, Cytoplasmic and Nuclear; D018124:Receptors, Tumor Necrosis Factor; C506030:TNFRSF11B protein, human; D014157:Transcription Factors