Cloning and biochemical characterization of FOX-5, an AmpC-type plasmid-encoded beta-lactamase from a New York City Klebsiella pneumoniae clinical isolate.
Klebsiella pneumoniae 5064, isolated in New York, carried plasmid-mediated resistance to multiple beta-lactams and was unresponsive to clavulanic acid. The beta-lactamase gene responsible for cephalosporin resistance encoded FOX-5, with 96 to 97% amino acid identities to other members of the FOX family of beta-lactamases. The bla(FOX-5) coding region was located next to a transposase gene from the Aeromonas salmonicida insertion element ISAS2.
Queenan
A M
AM
The R.W. Johnson Pharmaceutical Research Institute, Raritan, New Jersey 08869, USA. aqueenan@prius.jnj.com
Jenkins
S
S
Bush
K
K
D000368:Aged; D000369:Aged, 80 and over; D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D019818:Clavulanic Acid / Q000494:pharmacology; D003001:Cloning, Molecular; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D006801:Humans; D007525:Isoelectric Focusing; D007710:Klebsiella Infections / Q000382:microbiology*; D007711:Klebsiella pneumoniae / Q000235:genetics*; D008969:Molecular Sequence Data; D009519:New York City; D010957:Plasmids / Q000235:genetics; D013379:Substrate Specificity; D018440:beta-Lactam Resistance; D065093:beta-Lactamase Inhibitors; D001618:beta-Lactamases / Q000235:genetics*
D000900:Anti-Bacterial Agents; D004791:Enzyme Inhibitors; D065093:beta-Lactamase Inhibitors; D019818:Clavulanic Acid; C444464:beta-lactamase FOX-5; D001618:beta-Lactamases