Determination of cell viability in single or mixed samples using capillary electrophoresis laser-induced fluorescence microfluidic systems.
The advent of high-efficiency microbial separations will have a profound effect on both chemistry and microbiology. For the first time, it appears that it may be possible to obtain qualitative and quantitative information on microbial systems with the accuracy, precision, speed, and throughput that currently is found for chemical systems. Recently it was suggested that an analytical separations-based approach for determining the viability of cells would be advantageous. The feasibility of such an approach is demonstrated using CE-LIF of two bacteria and yeast. The analytical procedures and figures of merit are outlined. High-throughput analyses and evaluation of microorganisms now appear to be possible.
Armstrong
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Department of Chemistry, Iowa State University, Ames 50011, USA.
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D001644:Bifidobacterium / Q000166:cytology* / Q000302:isolation & purification; D019075:Electrophoresis, Capillary / Q000379:methods*; D007779:Lactobacillus acidophilus / Q000166:cytology* / Q000302:isolation & purification; D008828:Microbiological Techniques / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000302:isolation & purification; D013056:Spectrophotometry, Ultraviolet / Q000379:methods