[Gene expression of beta-adrenoceptor signal transmitters in heart failure].
OBJECTIVE
To investigate the alteration in steady-state levels of messenger RNA(mRNA) of beta-adrenoceptor signal transmitters in heart failure.


METHODS
The reverse transcription polymerase chain reaction (RT-PCR) was used to assess gene expression in small quantity of circulatory lymphocytes. With selected oligonucleotide primers, we used quantitative RT-PCR to amplify mRNAs encoding beta 2-adrenergic receptor(beta 2-AR), adenylate cyclase (AC), beta 2-adrenergic receptor kinase(beta-ARK), and beta-arrestin and cAMP response element binding protein (CREB) in 16 healthy subjects and 30 heart-failing patients.


RESULTS
The alteration of gene expression in heart failure appeared to be selective, the steady-state levels of mRNA increased significantly involving AC and the transcription factor, CREB; decreased significantly involving membrane receptor, beta 2-AR; unchanged significantly involving phosphorylating factors of beta-AR uncoupling, beta-ARK and beta-arrestin.


CONCLUSIONS
The aberrant gene expression of beta-adrenergic receptor might play an important role in the pathogenesis of heart failure.
Yu
X
X
Guangdong Provincial Cardiovascular Institute, Guangzhou 510080.
Lin
S
S
Wang
X
X
D000262:Adenylyl Cyclases / Q000096:biosynthesis* / Q000235:genetics; D000293:Adolescent; D000328:Adult; D000368:Aged; D017868:Cyclic AMP-Dependent Protein Kinases / Q000096:biosynthesis / Q000235:genetics; D005260:Female; D015870:Gene Expression; D006333:Heart Failure / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011943:Receptors, Adrenergic, beta / Q000096:biosynthesis* / Q000235:genetics; D018343:Receptors, Adrenergic, beta-2 / Q000096:biosynthesis* / Q000235:genetics; D015398:Signal Transduction; D051552:beta-Adrenergic Receptor Kinases
D012333:RNA, Messenger; D011943:Receptors, Adrenergic, beta; D018343:Receptors, Adrenergic, beta-2; D017868:Cyclic AMP-Dependent Protein Kinases; D051552:beta-Adrenergic Receptor Kinases; D000262:Adenylyl Cyclases