[Study on electrochemical behavior of HL-60 cells during the etoposide-inducing apoptosis].
OBJECTIVE
To study the electrochemical behavior of apoptotic HL-60 cells induced with etoposide.


METHODS
Electrochemical cytosensor was used to monitor HL-60 cells treated with etoposide.


RESULTS
The peak current and electron transfer rate decreased as etoposide dosage increased. The decrease appeared at 2 micrograms/ml etoposide treatment and was remarkable at 20 micrograms/ml, which occurred before the typical changes of cell morphology and DNA contents for apoptosis. FCM-DNA analysis showed that the apoptotic cells were of 10.5%-20% while the peak current decreased from 1.8 microA to 0.4 microA after 2.3 and 4 hours treatment with 200 micrograms/ml etoposide, and the peak current after 4 hours treatment was 5 times lower than that before induction.


CONCLUSIONS
The changes of electrochemical behavior during the early stage of apoptosis occurred earlier and could be accumulated.
Ren
X
X
Institute of Hematology, Beijing Medical University, Beijing 100044.
Wang
D
D
Li
H
H
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D004563:Electrochemistry; D005047:Etoposide / Q000494:pharmacology*; D018922:HL-60 Cells / Q000473:pathology; D006801:Humans
D000972:Antineoplastic Agents, Phytogenic; D005047:Etoposide