[Comparison of the effects of gonadotropic preparations of the carp and stellate sturgeon pituitaries on in vivo and in vitro oocyte maturation in the Siberian sturgeon Acipenser baeri brandt].
Injections of 2.5 mg/kg of stellate sturgeon (Acipenser stellatus Pall.) pituitary extract and 5 mg/kg of carp (Cyprinus carpio L.) pituitary extract in Siberian sturgeon (Acipenser baeri Brandt) females did not reveal significant differences in the effects of these preparations. There were no differences in the percentage of females that responded by ovulation, duration of the period from injection to ovulation, rate of ovulation, or quality of mature eggs as estimated by the percentage of fertilization or percentage of normal embryos at the small yolk plug stage. Thus, an insufficient efficiency in the artificial reproduction of the Siberian sturgeon grown in captivity is not related to the use of the carp pituitary preparation as a stimulus. Estimation of the ratio of specific activities of the pituitary extracts and purified gonadotropins of the stellate sturgeon and carp by in vitro oocyte maturation has shown that it varies within wide limits as a function of the medium composition and physiological state of follicles. Hence, the ratio of activities of the gonadotropins of different species as determined by in vitro maturation of sturgeon oocytes may markedly differ from that upon injection of these preparations in breeders.
Goncharov
B F
BF
Kol'tsov Institute of Developmental Biology, Russian Academy of Sciences, ul. Vavilova 26, Moscow, 119991 Russia.
Viio
P
P
Menn
F L
FL
D000818:Animals; D002347:Carps; D003470:Culture Media; D004625:Embryo, Nonmammalian / Q000502:physiology; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods; D005399:Fishes / Q000196:embryology / Q000502:physiology; D006062:Gonadotropins / Q000494:pharmacology*; D008297:Male; D009865:Oocytes / Q000187:drug effects* / Q000502:physiology*; D010062:Ovulation Induction; D010902:Pituitary Gland / Q000737:chemistry*; D014020:Tissue Extracts / Q000494:pharmacology*
D003470:Culture Media; D006062:Gonadotropins; D014020:Tissue Extracts
Sravnenie deistviia gonadotrpnykh preparatov gipofiza karpa i sevriugi na sozrevanie ootsitov cibirskogo ocetra Acipenser baeri brandt in vivo i in vitro.