Rapid enantiomeric quantification of an antiviral nucleoside agent (D,L-FMAU, 2'-fluoro-5-methyl-beta,D,L-arabinofurano-syluracil) by mass spectrometry.
A novel mass spectrometric method is applied to rapid, accurate (<1%) quantification of chiral Clevudine (L-FMAU, 2'-fluoro-5-methyl-beta,L-arabinofuranosyluracil), a potent antiviral nucleoside agent against hepatitis B virus. Transition metal bound complex ions containing the chiral drug are generated by electrospray ionization mass spectrometry and subjected to collision-induced dissociation. The ratio of the two competitive dissociation rates is related to the enantiomeric composition of the drug mixture, allowing the determination of enantiomeric contamination in the drug.
Tao
W A
WA
Wu
L
L
Cooks
R G
RG
Wang
F
F
Begley
J A
JA
Lampert
B
B
D000998:Antiviral Agents / Q000737:chemistry*; D001086:Arabinofuranosyluracil / Q000031:analogs & derivatives / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D013237:Stereoisomerism
D000998:Antiviral Agents; D001086:Arabinofuranosyluracil; C034935:clevudine