[Cell proliferation in human arthrotic joint cartilage].
OBJECTIVE
Osteoarthritic (OA) cartilage is histologically characterized by the appearance of cell clusters, which are probably generated by mitotic cell division. The aim of this study was to analyze the distribution and amount of proliferating chondrocytes in healthy and osteoarthritic cartilage systematically.


METHODS
6 normal and 43 osteoarthritic cartilage/bone samples were obtained during autopsies or total knee replacements. The cartilage specimens were stained with safranin-o and scored according to the Mankin-System. Proliferating chondrocytes were identified by immunohistochemical detection of the antigen PCNA. The number of proliferating chondrocytes was determined by counting 100 chondrocytes in each cartilage layer.


RESULTS
In normal and osteoarthritic human cartilage, proliferating chondrocytes were detected. The amount of proliferating chondrocytes increased during the progression of OA cartilage changes. Expression of PCNA was determined in single chondrocytes and clustered chondrocytes. Chondrocyte cell division was activated specifically in cartilage with severe OA changes.


CONCLUSIONS
Osteoarthritic chondrocytes, which are known to increase the synthesis of different matrix proteins, show the phenomenon of mitotic cells division. Whether the increased amount of proliferating chondrocytes fails to compensate the rate of apoptotic chondrocytes, or whether the generation of cell clusters represents only a fruitless repair effort is a subject of recent research. However, the induction of chondrocyte proliferation is a potential target in the treatment of OA.
Pfander
D
D
Abteilung für Orthopädische Rheumatologie, Orthopädische Klinik mit Poliklinik, Friedrich-Alexander-Universität Erlangen-Nümberg.
Körtje
D
D
Weseloh
G
G
Swoboda
B
B
D002358:Cartilage, Articular / Q000473:pathology*; D002452:Cell Count; D002455:Cell Division / Q000502:physiology*; D019902:Chondrocytes / Q000473:pathology*; D006801:Humans; D007124:Immunoenzyme Techniques; D007719:Knee Joint / Q000473:pathology; D020370:Osteoarthritis, Knee / Q000145:classification / Q000473:pathology*; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D012016:Reference Values
D018809:Proliferating Cell Nuclear Antigen
Zellproliferation im humanen arthrotischen Gelenkknorpel.