title: Mutagenesis in the post genomics era: tools for generating insertional mutations in the lactic acid bacteria.
abstract: The increasing availability of whole genome sequences has increased the demand for effective tools to generate insertional mutations in the lactic acid bacteria (LAB). Several novel approaches, such as shuttle-, transposome- and intron-based mutagenesis methods, are possible additions to the existing repertoire of transposon- and recombination-based tools available for mutagenesis of LAB.
authors.affiliation: Department of Viticulture and Enology, University of California, 1 Shields Avenue, Davis, CA 95616-8749, USA. damills@ucdavis.edu
authors.forename: D A
authors.initials: DA
authors.lastname: Mills
mesh_terms: D001419:Bacteria / Q000235:genetics*; D002876:Chromosomes, Bacterial; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics; D005524:Food Technology; D016680:Genome, Bacterial; D023281:Genomics; D007438:Introns; D019344:Lactic Acid / Q000378:metabolism; D016254:Mutagenesis, Insertional / Q000379:methods*; D016297:Mutagenesis, Site-Directed; D011995:Recombination, Genetic
chemical_list: D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D019344:Lactic Acid