Bioconversion of nitriles by Candida guilliermondii CCT 7207 cells immobilized in barium alginate.
Nitrile degradation by Candida guilliermondii CCT 7207 using free and immobilized cell systems was compared. Different specific growth rates were observed for immobilized (mumax=0.021 h(-1)) and the free cells (mumax=0.029 h(-1)). The maximum specific rate of acetic acid formation was 0.387 h(-1) and 0.266 h(-1) for free and immobilized cells, respectively. Cell adhesion to the support materials was confirmed by scanning electron microscopy. When immobilized, the yeast was able to use high nitrile and amide concentrations (aliphatic and aromatic) as nitrogen sources. The results suggest that C. guilliermondii CCT 7207 presents a physiological pattern potentially useful for the bioremediation of polluted environments or for the bioproduction of amides and organic acid of high commercial value.
Dias
J C
JC
Departamento de Microbiologia, ICB/UFMG, Belo Horizonte, Brazil. linardiv@mono.icb.ufmg.br
Rezende
R P
RP
Linardi
V R
VR
D000464:Alginates; D017609:Barium Compounds; D001673:Biodegradation, Environmental; D002175:Candida / Q000254:growth & development* / Q000378:metabolism* / Q000648:ultrastructure; D018914:Cells, Immobilized; D002712:Chlorides; D003470:Culture Media; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D008854:Microscopy, Electron; D009570:Nitriles / Q000378:metabolism*
D000464:Alginates; D017609:Barium Compounds; D002712:Chlorides; D003470:Culture Media; D006603:Hexuronic Acids; D009570:Nitriles; C024986:barium chloride; D020723:Glucuronic Acid