Migration, integration, and differentiation of hippocampus-derived neurosphere cells after transplantation into injured rat spinal cord.
Hippocampus-derived neurospheres were prepared from transgenic rat fetuses expressing green fluorescent protein (GFP), and transplanted into an alginate-filled lesion of young rat spinal cord. One, two and four weeks after transplantation, a large number of grafted cells survived, many of which expressed immunoreactivity for glial fibrillary acidic protein, and a few expressed immunoreactivity for beta-tubulin III. The grafted cells closely attached to the host tissue including astrocytes at the border of the lesion. It was notable that numerous GFP-positive cells had migrated within host spinal cord tissue up to 2 mm away from the implanted site 4 weeks postoperation. These results demonstrate that rat fetal hippocampus-derived neurosphere cells could survive, differentiate, extensively migrate, and integrate well into the host spinal cord tissue.
Wu
S
S
Department of Plastic and Reconstructive Surgery, Kyoto University Graduate School of Medicine, Kyoto 606-8507, Japan.
Suzuki
Y
Y
Kitada
M
M
Kitaura
M
M
Kataoka
K
K
Takahashi
J
J
Ide
C
C
Nishimura
Y
Y
D000464:Alginates / Q000494:pharmacology; D000818:Animals; D030801:Animals, Genetically Modified; D016380:Brain Tissue Transplantation / Q000379:methods*; D002454:Cell Differentiation / Q000502:physiology*; D002465:Cell Movement / Q000502:physiology*; D002478:Cells, Cultured / Q000166:cytology / Q000378:metabolism / Q000637:transplantation; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D006085:Graft Survival / Q000502:physiology*; D049452:Green Fluorescent Proteins; D006624:Hippocampus / Q000166:cytology / Q000196:embryology / Q000637:transplantation*; D007150:Immunohistochemistry; D007202:Indicators and Reagents / Q000378:metabolism; D008164:Luminescent Proteins / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000166:cytology / Q000378:metabolism / Q000601:surgery; D013119:Spinal Cord Injuries / Q000601:surgery*; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism; D014404:Tubulin / Q000378:metabolism
D000464:Alginates; D005904:Glial Fibrillary Acidic Protein; D007202:Indicators and Reagents; D008164:Luminescent Proteins; D014404:Tubulin; D049452:Green Fluorescent Proteins