Identification of heme oxygenase in human endometrium.
The aim of the present study was to investigate the presence of heme oxygenase (HO)-1 and HO-2 in human endometrium at various stages of the menstrual cycle using RT-PCR, Western blotting, and immunohistochemistry. RT-PCR detected mRNA for HO-1 and HO-2 in human endometrium at all stages of the menstrual cycle. Western blotting also revealed the expression of the two distinct HO proteins throughout the menstrual cycle. HO-1 was constitutively expressed, whereas HO-2 expression was apparently greater in the secretory phase than in the menstrual and proliferative phases. Immunohistochemistry showed that distribution of the two HO isoforms had distinct topographic patterns: HO-1 was observed in endometrial epithelial cells and macrophages, whereas HO-2 was found in endothelial cells and smooth muscle cells of blood vessels in the endometrium. The detection of mRNA and protein for HO-1 and HO-2 in normal human endometrium suggests that the carbon monoxide/HO system may play a role in the local control of endometrial function.
Yoshiki
N
N
Comprehensive Reproductive Medicine, Regulation of Internal Environment and Reproduction, Graduate School, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8519, Japan. n.yoshiki.gyne@med.tmd.ac.jp
Kubota
T
T
Aso
T
T
D015703:Antigens, CD / Q000032:analysis; D015214:Antigens, Differentiation, Myelomonocytic / Q000032:analysis; D015153:Blotting, Western; D002248:Carbon Monoxide / Q000378:metabolism; D004717:Endometrium / Q000201:enzymology*; D005260:Female; D006419:Heme Oxygenase (Decyclizing) / Q000032:analysis* / Q000235:genetics; D051547:Heme Oxygenase-1; D006801:Humans; D007150:Immunohistochemistry; D008565:Membrane Proteins; D009569:Nitric Oxide / Q000502:physiology; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000097382:CD68 Molecule
D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D002248:Carbon Monoxide; D006419:Heme Oxygenase (Decyclizing); D051547:Heme Oxygenase-1; D008565:Membrane Proteins; D009569:Nitric Oxide; D012333:RNA, Messenger; C067980:CD68 antigen, human; D000097382:CD68 Molecule; C496541:HMOX1 protein, human; C105245:heme oxygenase-2