title: Family of class 1 integrons related to In4 from Tn1696.
abstract: The class 1 integron In28, found in the multidrug resistance transposon Tn1403, was found to be located in the res site of the backbone transposon and is flanked by a 5-bp direct duplication, indicating that it reached this position by transposition. In28 has a backbone structure related to that of In4, but has lost internal sequences, including the sul1 gene, due to an IS6100-mediated deletion. In28 also lacks the partial copy of IS6100 found in In4 and contains different gene cassettes, blaP1, cmlA1, and aadA1. In1, the class 1 integron found in the multidrug resistance plasmid R46, is also located in a putative res site and belongs to the In4 group. In1 has a shorter internal deletion than In28 and has also lost one end. Additional integrons with structures related to In4 were also found in databases, and most of them had also lost either one end or internal regions or both. Tn610 belongs to this group.
authors.affiliation: CSIRO Molecular Science, North Ryde, New South Wales 2113, Australia.
authors.forename: S R
authors.initials: SR
authors.lastname: Partridge
authors.forename: G D
authors.initials: GD
authors.lastname: Recchia
authors.forename: H W
authors.initials: HW
authors.lastname: Stokes
authors.forename: R M
authors.initials: RM
authors.lastname: Hall
mesh_terms: D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004268:DNA-Binding Proteins / Q000235:genetics*; D004351:Drug Resistance; D004926:Escherichia coli / Q000235:genetics; D016023:Integrins / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; C090091:In4 complex; D016023:Integrins