A hybrid bacterial replication origin.
We constructed a hybrid replication origin that consists of the main part of oriC from Escherichia coli, the DnaA box region and the AT-rich region from Bacillus subtilis oriC. The AT-rich region could be unwound by E. coli DnaA protein, and the DnaB helicase was loaded into the single-stranded bubble. The results show that species specificity, i.e. which DnaA protein can do the unwinding, resides within the DnaA box region of oriC.
Seitz
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Max-Planck-Institut für molekulare Genetik, Ihnestrasse 73, D-14195 Berlin, Germany.
Welzeck
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Messer
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D001412:Bacillus subtilis / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D018983:DNA Footprinting; D004265:DNA Helicases / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics*; D053488:DnaB Helicases; D004926:Escherichia coli / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids / Q000378:metabolism; D011196:Potassium Permanganate / Q000494:pharmacology; D018741:Replication Origin; D013045:Species Specificity
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C066312:DnaA protein, Bacteria; D011196:Potassium Permanganate; D004265:DNA Helicases; D053488:DnaB Helicases