Sequences adjacent to oriP improve the persistence of Epstein-Barr virus-based episomes in B cells.
Epstein-Barr virus (EBV) oriP and the EBV nuclear antigen 1 (EBNA-1) protein allow persistence of EBV-based episomes. A nuclear matrix attachment region (MAR) spans oriP and the adjacent region of the EBV genome containing the EBV-expressed RNAs. Here, we show that episomes with the MAR are retained significantly more efficiently in EBV-positive B cells than episomes containing oriP alone.
White
R E
RE
Wellcome Trust Centre for Human Genetics, University of Oxford, Headington, Oxford OX3 7BN, United Kingdom.
Wade-Martins
R
R
James
M R
MR
D001287:Attachment Sites, Microbiological; D001402:B-Lymphocytes / Q000821:virology*; D005814:Genes, Viral; D004854:Herpesvirus 4, Human / Q000235:genetics*; D006801:Humans; D015530:Nuclear Matrix / Q000821:virology; D010957:Plasmids; D012367:RNA, Viral / Q000235:genetics; D018741:Replication Origin; D014407:Tumor Cells, Cultured
C099537:Epstein-Barr virus encoded RNA 1; C106732:Epstein-Barr virus encoded RNA 2; D012367:RNA, Viral