[Reversal effect of Ys-96, a bisbenzylisoquinoline, on adriamycin or vincristine resistance in human cancer cells in vitro].
OBJECTIVE
Reversal effect of Ys-96, a bisbenzylisoquinoline, on the resistance of human cancer cells to adriamycin or vincristine was studied in vitro.


METHODS
In an in vitro culture system of human cancer cells MCF-7 and its adriamycin-resistant line MCF-7/Ad or KB and its vincristine-resistant line KBv200, the sensitivity(IC50) of the resistant cell lines to adriamycin or vincristine was evaluated with a MTT assay.


RESULTS
IC50 value of adriamycin or vincristine in combination with Ys-96 at a concentration of 1.00 mumol/L against MCF-7/Ad or KBv200 was found to be close to that of adriamycin alone or vincristine alone against the sensitive cell line MCF-7 or KB.


CONCLUSIONS
The drug resistance of MCF-7/Ad or KBv200 could be essentially reversed by Ys-96 at a concentration of 1.00 mumol/L.
Ye
Z
Z
Institute of Chinese Materia Medica, China Academy of Traditional Chinese Medicine, Beijing 100700.
Sun
A
A
Li
C
C
Xue
B
B
Wang
Z
Z
Tong
Y
Y
Feng
H
H
Liang
A
A
Wang
J
J
Yang
Q
Q
D044182:Benzylisoquinolines; D001943:Breast Neoplasms / Q000473:pathology; D004317:Doxorubicin / Q000494:pharmacology*; D019008:Drug Resistance, Neoplasm; D004357:Drug Synergism; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006801:Humans; D007546:Isoquinolines / Q000494:pharmacology*; D007624:KB Cells / Q000187:drug effects; D014407:Tumor Cells, Cultured / Q000187:drug effects; D014750:Vincristine / Q000494:pharmacology
D044182:Benzylisoquinolines; D004365:Drugs, Chinese Herbal; D007546:Isoquinolines; D014750:Vincristine; D004317:Doxorubicin