[Expressions of cellular M-CSF and M-CSFR in hematopoietic cells].
OBJECTIVE
To study the distribution of cellular M-CSF and its receptor in abnormal hematopoiesis.


METHODS
Six hematopoietic cell lines, 144 bone marrow (BM) and 160 peripheral blood (PB) samples were investigated with ABC immunoperoxidase assay.


RESULTS
Cellular M-CSF and/or M-CSFR were positive in all the 6 hematopoietic cell lines, a few normal BM(4 cases) and PB (32 cases) samples, hematological malignancies and a few benign hematological disorder samples. M-CSF and M-CSFR were found mainly in granulocytic lineage cells, seldom in erythroid and megakaryocytic lineage cells and never in lymphocytes. M-CSF and M-CSFR usually presented in cytoplasma and membrane and in some cases in nuclei.


CONCLUSIONS
Cellular M-CSF and M-CSFR is widely distributed in abnormal hematopoiesis, and is especially high in myeloid leukemias.
Zhang
Q
Q
Institute of Hematology, CAMS, PUMC, Tianjin 300020.
Xue
Y
Y
Song
Y
Y
D018922:HL-60 Cells / Q000378:metabolism; D006410:Hematopoiesis / Q000502:physiology*; D006801:Humans; D020014:K562 Cells / Q000378:metabolism; D016173:Macrophage Colony-Stimulating Factor / Q000096:biosynthesis*; D016186:Receptor, Macrophage Colony-Stimulating Factor / Q000096:biosynthesis*; D020298:U937 Cells / Q000378:metabolism
D016173:Macrophage Colony-Stimulating Factor; D016186:Receptor, Macrophage Colony-Stimulating Factor