[Transfection of Fas(CD95) gene into HL-60 cell and its role in rhG-CSF-induced apoptosis].
OBJECTIVE
To explore the role of Fas gene in rhG-CSF-induced apoptosis.


METHODS
Mediated by lipofection (DOTAP), Fas cDNA was transferred into HL-60 cells. In situ hybridization, Western blotting and FCM analysis were used to demonstrate the successful transfection. The apoptosis percentages of HL-60 and Fas transfected HL-60 cells were compared after coculturing with rhG-CSF at the same final concentration.


RESULTS
After cocultured with rhG-CSF (10 ng/ml) for 96 hours, the apoptosis percentage of Fas transferred HL-60 cells was significantly higher than that of HL-60 cells, and the Fas expressions could be up-regulated by rhG-CSF.


CONCLUSIONS
Fas is an apoptosis-induction oncogene, and rhG-CSF induces apoptosis of HL-60 cells through Fas signalling pathway.
Song
Y
Y
Shandong Provincial Hospital, Jinan 250021.
Xu
G
G
Zhang
L
L
D017209:Apoptosis; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D018922:HL-60 Cells / Q000473:pathology*; D006801:Humans; D011994:Recombinant Proteins; D014162:Transfection; D019014:fas Receptor / Q000096:biosynthesis* / Q000235:genetics
D011994:Recombinant Proteins; D019014:fas Receptor; D016179:Granulocyte Colony-Stimulating Factor