title: The cyclin B1 gene is actively transcribed during mitosis in HeLa cells.
abstract: In mammalian cells, the expression level of the cyclin B1 gene plays a critical role in the progression through mitosis. Here we demonstrate that the transcriptional activity of the human cyclin B1 promoter, as well as the rate of gene transcription, is high during mitosis. Indeed, the cyclin B1 promoter maintains an open chromatin configuration at the mitotic stage. Consistent with this, we show that the cyclin B1 promoter is occupied and bound to NF-Y during mitosis in vivo. Our results provide the first example of RNA polymerase II-dependent transcription during mitosis in mammalian cells.
authors.affiliation: Dipartimento di Oncologia Sperimentale, Istituto Regina Elena, Via delle Messi D'Oro 156, 00158 Rome, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Sciortino
authors.forename: A
authors.initials: A
authors.lastname: Gurtner
authors.forename: I
authors.initials: I
authors.lastname: Manni
authors.forename: G
authors.initials: G
authors.lastname: Fontemaggi
authors.forename: A
authors.initials: A
authors.lastname: Dey
authors.forename: A
authors.initials: A
authors.lastname: Sacchi
authors.forename: K
authors.initials: K
authors.lastname: Ozato
authors.forename: G
authors.initials: G
authors.lastname: Piaggio
mesh_terms: D015152:Blotting, Northern; D023081:CCAAT-Binding Factor / Q000378:metabolism; D002843:Chromatin / Q000378:metabolism; D003432:Cross-Linking Reagents / Q000494:pharmacology; D019926:Cyclin B / Q000096:biosynthesis* / Q000235:genetics*; D056744:Cyclin B1; D018076:DNA, Complementary / Q000378:metabolism; D016195:G2 Phase; D006367:HeLa Cells; D006801:Humans; D008938:Mitosis; D008957:Models, Genetic; D011233:Precipitin Tests; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D011487:Protein Conformation; D014158:Transcription, Genetic
chemical_list: D023081:CCAAT-Binding Factor; C534699:CCNB1 protein, human; D002843:Chromatin; D003432:Cross-Linking Reagents; D019926:Cyclin B; D056744:Cyclin B1; D018076:DNA, Complementary