title: A monoclonal antibody specific for Pseudomonas aeruginosa amidase.
abstract: Amidase from Pseudomonas aeruginosa was purified by anionic exchange chromatography and used to immunise female Balb/c mice. Monoclonal antibodies (MAbs) were raised by hybridoma technology using Sp2/0 myeloma cells as fusion partner. A selected IgM subclass MAb was purified from in vitro hybridoma cell line supernatant by a two-step anionic exchange chromatography. The MAb was specific for amidase from P. aeruginosa as determined by Western blotting and recognized the native and denatured forms of the enzyme.
authors.affiliation: INETI, Departmento de Biotecnologia, UTPAM, Estrada do Paço do Lumiar, 1649-038, Lisboa, Portugal. carlos.novo@mail.ineti.pt
authors.forename: C
authors.initials: C
authors.lastname: Novo
authors.forename: A
authors.initials: A
authors.lastname: Karmali
authors.forename: A
authors.initials: A
authors.lastname: Clemente
authors.forename: P R
authors.initials: PR
authors.lastname: Brown
mesh_terms: D000581:Amidohydrolases / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D000918:Antibody Specificity; D015153:Blotting, Western; D002852:Chromatography, Ion Exchange; D005260:Female; D007075:Immunoglobulin M / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D011550:Pseudomonas aeruginosa / Q000201:enzymology*
chemical_list: D000911:Antibodies, Monoclonal; D007075:Immunoglobulin M; D000581:Amidohydrolases; C019665:amidase