title: Aminoglycoside-streptothricin resistance gene cluster aadE-sat4-aphA-3 disseminated among multiresistant isolates of Enterococcus faecium.
abstract: Seventy-two Enterococcus faecium isolates of different origins highly resistant to nourseothricin and streptomycin were studied. Sequencing of a genomic fragment from two isolates identified a gene cluster, aadE-sat4-aphA-3, which has been isolated recently in staphylococci and Campylobacter coli. Patterns of digested PCR products of aadE-sat4-aphA-3 were identical for all isolates.
authors.affiliation: Wernigerode Branch, Robert Koch-Institute, 38855 Wernigerode, Germany. wernerg@rki.de
authors.forename: G
authors.initials: G
authors.lastname: Werner
authors.forename: B
authors.initials: B
authors.lastname: Hildebrandt
authors.forename: W
authors.initials: W
authors.lastname: Witte
mesh_terms: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D004351:Drug Resistance; D016984:Enterococcus faecium / Q000187:drug effects* / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013309:Streptothricins / Q000494:pharmacology*
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D013309:Streptothricins