Targeted disruption of a pupal hemocyte protein of Sarcophaga by RNA interference.
Previously, we purified a transmembrane protein with a molecular mass of 120 kDa (p120) that is exclusively expressed in pupal hemocytes of Sarcophaga. In this study, we demonstrated that double-stranded RNA (dsRNA) injected into the larval body cavity effectively inhibited the expression of p120 in pupal hemocytes. Thus, RNA interference (RNAi) was found to be a useful technique for creating pupal hemocytes with a loss-of-function of a specific protein. The p120-less pupal hemocytes generated by RNAi were found to have lost the ability to take up acetylated low density lipoprotein, indicating that p120 is a scavenger receptor specifically expressed on the surface of pupal hemocytes.
Nishikawa
T
T
Graduate School of Pharmaceutical Sciences, University of Tokyo, Bunkyo-ku, Tokyo, Japan.
Natori
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S
D000818:Animals; D015153:Blotting, Western; D004175:Diptera; D006434:Hemocytes / Q000166:cytology / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D007104:Immune Adherence Reaction; D019476:Insect Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D007814:Larva / Q000187:drug effects / Q000378:metabolism; D008077:Lipoproteins, LDL / Q000378:metabolism; D008565:Membrane Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011679:Pupa / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D012330:RNA, Double-Stranded / Q000494:pharmacology*; D011971:Receptors, Immunologic / Q000096:biosynthesis / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D018110:Receptors, Lipoprotein; D051116:Receptors, Scavenger; D051122:Scavenger Receptors, Class B
D019476:Insect Proteins; D008077:Lipoproteins, LDL; D008565:Membrane Proteins; D012330:RNA, Double-Stranded; D011971:Receptors, Immunologic; D018110:Receptors, Lipoprotein; D051116:Receptors, Scavenger; C454739:Scarb1 protein, mouse; D051122:Scavenger Receptors, Class B; C032629:acetyl-LDL