Gene expression monitoring in soils by mRNA analysis and gene lux fusions.
Two methods recently developed to monitor the gene expression of microbial communities in soil are the extraction and detection of messenger RNA from soil microorganisms and the construction and use of lux-based bioreporter strains. The goal of these approaches is to assess microbial activity in natural and impacted soil environments.
Sayler
G S
GS
Department of Microbiology, University of Tennessee, 676 Dabney Hall, Knoxville TN 37996-1605, USA. sayler@utk.edu
Fleming
J T
JT
Nivens
D E
DE
D019677:Artificial Gene Fusion; D020869:Gene Expression Profiling / Q000379:methods*; D005798:Genes, Bacterial; D017930:Genes, Reporter; D008164:Luminescent Proteins / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012988:Soil Microbiology
D008164:Luminescent Proteins; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins