title: Purification of microcystins by DEAE and C(18) cartridge chromatography.
abstract: Microcystins (MCs) were purified by DEAE and C(18) cartridge chromatography. Addition of EtOH to the eluents (20%) in DEAE chromatography gave higher resolution than no addition of EtOH. The chromatogram showed three peaks: MC-LR; MC-LY and MC-LF; MC-LW. MC-LR and MC-LW were obtained by one step chromatography with purity of 96 and 88%, respectively. The separation of MC-LF and MC-LW with DEAE chromatography was better than that with reversed-phase chromatography. MC-LY and MC-LF were separated with C(18) cartridge. On the chromatogram, there were three peaks consisting of MC-LY (81% purity), MC-LF (86%), and an unknown compound which was considered as a MC variant judging from the results in HPLC/PDA, FAB-MS, and 1H NMR analyses, but the structure could not be determined. It is concluded that the combination of DEAE and C(18) cartridge chromatography would be a practical approach for the purification of various MCs.
authors.affiliation: School of Marine Science and Technology, Tokai University, 3-20-1 Orido, Shimizu, 424-8610, Shizuoka, Japan.
authors.forename: Kazunori
authors.initials: K
authors.lastname: Saito
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ishii
authors.forename: Fumiko
authors.initials: F
authors.lastname: Nishida
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Saito
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Abe
authors.forename: Yoshimasa
authors.initials: Y
authors.lastname: Toyota
mesh_terms: D001427:Bacterial Toxins / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D000458:Cyanobacteria / Q000378:metabolism*; D000431:Ethanol / Q000737:chemistry; D066298:In Vitro Techniques; D052998:Microcystins; D010456:Peptides, Cyclic / Q000302:isolation & purification*
chemical_list: D001427:Bacterial Toxins; D052998:Microcystins; D010456:Peptides, Cyclic; D000431:Ethanol; C078588:microcystin