Recombinant canine IL-13 receptor alpha2-Fc fusion protein inhibits canine allergen-specific-IgE production in vitro by peripheral blood mononuclear cells from allergic dogs.
Human IL-13, like IL-4, is involved in the regulation of B-cell development, IgE synthesis and allergic responses. However, because IL-13 does not affect either murine Ig class switching or IgE production in vitro, the use of murine models to study the role of IL-13 in IgE-mediated diseases has been limited. In this communication, we report that recombinant protein of canine IL-13 (rcaIL-13) stimulates production of allergen-specific-IgE in vitro by peripheral blood mononuclear cells (PBMC) from flea allergen-sensitized dogs, and that this stimulation activity is specifically inhibited by recombinant protein of canine IL-13Ralpha2 and Fc fragment of canine IgG heavy chain (rcaIL-13Ralpha2-Fc). The data suggest that the regulatory effects of IL-13 on IgE production in canine PBMC are similar to those reported in humans. Thus, canine IL-13 may be a central mediator of allergic diseases in dogs, and allergic dogs may be excellent models for research on IgE-mediated diseases in humans.
Tang
L
L
Heska Corporation, 1613 Prospect Parkway, Fort Collins, CO 80525, USA. tangl@heska.com
Boroughs
K L
KL
Morales
T
T
Stedman
K
K
Sellins
K
K
Clarke
K
K
McDermott
M
M
Yang
S
S
McCall
C
C
D000595:Amino Acid Sequence; D000818:Animals; D004283:Dog Diseases / Q000097:blood / Q000276:immunology*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D006967:Hypersensitivity / Q000097:blood / Q000276:immunology / Q000662:veterinary*; D007073:Immunoglobulin E / Q000096:biosynthesis* / Q000097:blood; D007141:Immunoglobulin Fc Fragments / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D007143:Immunoglobulin Heavy Chains / Q000235:genetics / Q000276:immunology; D018793:Interleukin-13 / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000276:immunology; D053661:Interleukin-13 Receptor alpha1 Subunit; D007963:Leukocytes, Mononuclear / Q000276:immunology* / Q000378:metabolism; D008969:Molecular Sequence Data; D018123:Receptors, Interleukin / Q000235:genetics / Q000276:immunology*; D053660:Receptors, Interleukin-13; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D017422:Sequence Analysis, DNA; D005423:Siphonaptera / Q000276:immunology; D014407:Tumor Cells, Cultured
C445241:IL-13 receptor alpha2-Fc fusion protein, Canis familiaris; C508181:IL13RA1 protein, human; D007141:Immunoglobulin Fc Fragments; D007143:Immunoglobulin Heavy Chains; D018793:Interleukin-13; D053661:Interleukin-13 Receptor alpha1 Subunit; D018123:Receptors, Interleukin; D053660:Receptors, Interleukin-13; D011993:Recombinant Fusion Proteins; D007073:Immunoglobulin E