title: Neutralization of a snake venom hemorrhagic metalloproteinase prevents coagulopathy after subcutaneous injection of Bothrops jararaca venom in rats.
abstract: Coagulopathy is one of the major complications following envenomations by crotalid and viperid snakes. The present study was undertaken to examine the effect of a hemorrhagic metalloproteinase in Bothrops jararaca venom, jararafibrase I (JF I), on the development of coagulopathy using rat snakebite model. Coagulation parameters were monitored after subcutaneous injection of B. jararaca crude venom, JF I-neutralized venom and purified JF I in rats. Crude venom induced unclottable blood and fibrinogen consumption, while JF I-neutralized venom and purified JF I did not induce coagulopathy. Plasma venom antigen level of rats given JF I-neutralized venom was lower than that of rats given crude venom. We conclude that venom hemorrhagic metalloproteinases play an important role in the development of coagulopathy through rapid spreading of venom coagulation components from the injected area into systemic circulation.
authors.affiliation: Department of Physiology, Miyazaki Medical College, 5200 Kihara, Kiyotake, 889-1692, Miyazaki, Japan. akeita@post.miyazaki-med.ac.jp
authors.forename: Keita
authors.initials: K
authors.lastname: Anai
authors.forename: Masahiko
authors.initials: M
authors.lastname: Sugiki
authors.forename: Etsuo
authors.initials: E
authors.lastname: Yoshida
authors.forename: Masugi
authors.initials: M
authors.lastname: Maruyama
mesh_terms: D000818:Animals; D000997:Antivenins / Q000494:pharmacology / Q000627:therapeutic use*; D001777:Blood Coagulation / Q000187:drug effects; D015153:Blotting, Western; D017837:Bothrops; D003435:Crotalid Venoms / Q000037:antagonists & inhibitors* / Q000201:enzymology / Q000276:immunology; D004195:Disease Models, Animal; D004591:Electrophoresis, Polyacrylamide Gel; D005343:Fibrinolytic Agents; D006470:Hemorrhage / Q000517:prevention & control* / Q000628:therapy; D007074:Immunoglobulin G / Q000627:therapeutic use; D008297:Male; D008666:Metalloendopeptidases / Q000037:antagonists & inhibitors* / Q000276:immunology; D011517:Prothrombin Time; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000997:Antivenins; D003435:Crotalid Venoms; D005343:Fibrinolytic Agents; D007074:Immunoglobulin G; D008666:Metalloendopeptidases; C076581:jararafibrase I