Localisation of mRNAs for diamine oxidase and histamine receptors H1 and H2, at the feto-maternal interface of human pregnancy.
OBJECTIVE
To localise mRNAs for the histamine receptors H1, H2 and H3, and for diamine oxidase, in the placenta and decidua of the human feto-maternal interface.


METHODS
Complementary DNA for each mRNA of interest was amplified by polymerase chain reaction. Sub-cloned sequences were used to prepare probes for in situ hybridisation, and these were employed to localise the expression of mRNAs for histamine receptors H1 and H2, and for diamine oxidase.


RESULTS
mRNA for histamine receptors H1 and H2, and for diamine oxidase could be detected at the feto-maternal interface of human pregnancy, and localised to both decidual and placental cells.


CONCLUSIONS
The co-expression of these receptors and DAO is consistent with a role for histamine at the feto-maternal interface of human pregnancy.
Brew
O B
OB
Wolfson Institute of Health Science, Thames Valley University, London, UK. obed.brew@tvu.ac.nk
Sullivan
M H
MH
D006631:Amine Oxidase (Copper-Containing) / Q000096:biosynthesis*; D003001:Cloning, Molecular; D046508:Culture Techniques; D018076:DNA, Complementary / Q000096:biosynthesis / Q000302:isolation & purification; D005260:Female; D005333:Fetus / Q000378:metabolism*; D006632:Histamine / Q000378:metabolism; D006801:Humans; D017403:In Situ Hybridization; D008431:Maternal-Fetal Exchange; D010920:Placenta / Q000201:enzymology / Q000378:metabolism*; D011247:Pregnancy; D012333:RNA, Messenger / Q000096:biosynthesis*; D011969:Receptors, Histamine H1 / Q000096:biosynthesis*; D011970:Receptors, Histamine H2 / Q000096:biosynthesis*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D018076:DNA, Complementary; D012333:RNA, Messenger; D011969:Receptors, Histamine H1; D011970:Receptors, Histamine H2; D006632:Histamine; D006631:Amine Oxidase (Copper-Containing)