An ultrasound assisted extraction of the available humic substance from marine sediments.
In this paper an ultrasound assisted procedure for the extraction of the bio-available fraction of humic substance in marine sediments is described. The proposed method is based on a preliminary 24 h 8 M HCl treatment already proposed in a previous paper [M. Mecozzi, E. Pietrantonio, M. Amici, Fres. Environ. Bull. 7 (1998) 605], followed by consecutive extractions by 0.5 M NaOH coupling to an ultrasonic treatment. The main advantages of the ultrasound procedure are the reduced times of extraction which take 30 min in contrast to the 24 h required by shaking method and the possibility to perform also the quantitative estimation of the extractable fraction of humic substance present in marine samples.
Mecozzi
M
M
Istituto Centrale per la Ricerca Scientifica e Tecnologica Applicata al Mare, Via di Casalotti n. 300, 00166 Roma, Italy. mecoma@rdn.it
Amici
M
M
Pietrantonio
E
E
Romanelli
G
G
D002241:Carbohydrates / Q000032:analysis; D003124:Colorimetry; D006812:Humic Substances / Q000302:isolation & purification*; D007202:Indicators and Reagents; D007700:Kinetics; D012623:Seawater / Q000032:analysis; D012987:Soil / Q000032:analysis; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D014465:Ultrasonics
D002241:Carbohydrates; D006812:Humic Substances; D007202:Indicators and Reagents; D012987:Soil