[Regulation of arsenic trioxide-inducing apoptosis].
OBJECTIVE
To explore the relationship among intercellular -SH, caspase, retinoic acid (RA) and arsenic trioxide(As2O3)-induced apoptosis.


METHODS
The in vitro effect of different thiols compounds, RA and caspase inhibitors on As2O3-induced apoptosis in NB4 and HL-60 cells was studied.


RESULTS
1. NAC completely blocked, BSO potentiated while MTG, BAL had no effect on As2O3-induced apoptosis. 2. Z-VAD.fmk blocked while Y-VAD.fmk had no effect on As2O3-induced apoptosis. 3. RA and As2O3 showed synergism in HL-60 cells, while showed antagonism in NB4 cell.


CONCLUSIONS
1. As2O3 binds with intracellular -SH, changes signal transduction, selectively activates caspase and causes apoptosis. 2. The regulating effect of RA on As2O3-induced apoptosis depends on cell types.
Huang
X
X
Institute of Hematology, Peoples' Hospital, Beijing Medical University, Beijing 100044.
D000208:Acute Disease; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D000077237:Arsenic Trioxide; D001152:Arsenicals / Q000494:pharmacology*; D061945:Caspase Inhibitors; D018922:HL-60 Cells / Q000473:pathology; D006801:Humans; D007951:Leukemia, Myeloid / Q000473:pathology*; D009842:Oligopeptides / Q000494:pharmacology*; D010087:Oxides / Q000494:pharmacology*; D014212:Tretinoin / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D001152:Arsenicals; D061945:Caspase Inhibitors; D009842:Oligopeptides; D010087:Oxides; C427308:benzoylcarbonyl-valyl-aspartyl-valyl-alanyl-aspartyl-fluoromethyl ketone; D014212:Tretinoin; D000077237:Arsenic Trioxide