Simultaneous determination of melatonin and pyridoxine in tablets by gas chromatography-mass spectrometry.
A gas chromatographic-mass spectrometric (GC-MS) method for the qualitative and quantitative determination of melatonin plus pyridoxine commercial tablets is described. Melatonin and pyridoxine were simultaneously determined by GC-MS after extraction from ground tablets with methanol and derivatization with N-methyl-N-N-trimethlylsilyltrifluoroacetamide (MSTFA). The mass chromatograms were generated using 232 m/z ion for melatonin and 280 m/z ion for pyridoxine, respectively. Splitless injection offers good reproducibility with a standard deviation of 2%. The developed method was applied to analyze the melatonin and pyridoxine content from two different tablet formulations. Also, recovery, detection and quantification limits are reported.
Nùñez-Vergara
L J
LJ
Laboratory of Bioelectrochemistry, Faculty of Chemical and Pharmaceutical Sciences, University of Chile, P.O. Box 233, Santiago, Chile. lnunez@ll.ciq.uchile.cl
Squella
J A
JA
Sturm
J C
JC
Baez
H
H
Camargo
C
C
D002138:Calibration; D008401:Gas Chromatography-Mass Spectrometry; D008550:Melatonin / Q000032:analysis*; D011736:Pyridoxine / Q000032:analysis*; D012015:Reference Standards; D015203:Reproducibility of Results; D013607:Tablets
D013607:Tablets; D008550:Melatonin; D011736:Pyridoxine