title: A transport metabolon. Functional interaction of carbonic anhydrase II and chloride/bicarbonate exchangers.
abstract: The cytoplasmic carboxyl-terminal domain of AE1, the plasma membrane chloride/bicarbonate exchanger of erythrocytes, contains a binding site for carbonic anhydrase II (CAII). To examine the physiological role of the AE1/CAII interaction, anion exchange activity of transfected HEK293 cells was monitored by following the changes in intracellular pH associated with AE1-mediated bicarbonate transport. AE1-mediated chloride/bicarbonate exchange was reduced 50-60% by inhibition of endogenous carbonic anhydrase with acetazolamide, which indicates that CAII activity is required for full anion transport activity. AE1 mutants, unable to bind CAII, had significantly lower transport activity than wild-type AE1 (10% of wild-type activity), suggesting that a direct interaction was required. To determine the effect of displacement of endogenous wild-type CAII from its binding site on AE1, AE1-transfected HEK293 cells were co-transfected with cDNA for a functionally inactive CAII mutant, V143Y. AE1 activity was maximally inhibited 61 +/- 4% in the presence of V143Y CAII. A similar effect of V143Y CAII was found for AE2 and AE3cardiac anion exchanger isoforms. We conclude that the binding of CAII to the AE1 carboxyl-terminus potentiates anion transport activity and allows for maximal transport. The interaction of CAII with AE1 forms a transport metabolon, a membrane protein complex involved in regulation of bicarbonate metabolism and transport.
authors.affiliation: Membrane Transport Group and Canadian Institutes of Health Research Group in Molecular Biology of Membrane Proteins, Department of Physiology, University of Alberta, Edmonton, Alberta T6G 2H7, Canada.
authors.forename: D
authors.initials: D
authors.lastname: Sterling
authors.forename: R A
authors.initials: RA
authors.lastname: Reithmeier
authors.forename: J R
authors.initials: JR
authors.lastname: Casey
mesh_terms: D000595:Amino Acid Sequence; D024402:Carbonic Anhydrase II / Q000037:antagonists & inhibitors / Q000378:metabolism*; D002460:Cell Line; D027963:Chloride-Bicarbonate Antiporters / Q000378:metabolism*; D004910:Erythrocyte Membrane / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D011485:Protein Binding
chemical_list: D027963:Chloride-Bicarbonate Antiporters; D024402:Carbonic Anhydrase II