title: A morphologic, biochemical, and biomechanical assessment of short-term effects of osteochondral autograft plug transfer in an animal model.
abstract: OBJECTIVE
  The objective of this study was to assess the short-term changes that occur after an osteochondral autograft plug transfer from the femoral trochlea to the medial femoral condyle in a goat model.
  
  
  METHODS
  Articular cartilage repair animal study.
  
  
  METHODS
  Six adult male goats were used in this study. Two 4.5-mm osteochondral plugs were transferred from the superolateral femoral trochlea to 2 recipient sites in the central portion of the medial femoral condyle for a survival period of 12 weeks. Postmortem, the global effects of the procedure were assessed by gross morphologic inspection and by analyzing the synovial DNA for inflammatory response. The recipient sites were also evaluated histologically and biomechanically. Metabolic activity was determined by (35)SO(4) uptake, and viability was assessed using a live/dead stain and by confocal laser microscopy.
  
  
  RESULTS
  There was no evidence of significant gross morphologic or histologic changes in the operative knee as a result of the osteochondral donor or recipient sites. The patella, tibial plateau, and medial meniscus did not show any increased degenerative changes as a result of articulating against the donor or recipient sites of the osteochondral autografts. Analysis of synovial DNA revealed no inflammatory response. Biomechanically, 6- to 7-fold greater stiffness was noted in the cartilage of the transferred plugs compared with the control medial femoral condyle. Furthermore, on histologic examination, the healing subchondral bone interface at the recipient site had increased density. Glycosaminoglycan synthesis as determined by (35)SO(4) uptake was upregulated in the transplanted cartilage plug relative to the contralateral control, showing a repair response at the site of implantation. And finally, confocal microscopy showed 95% viability of the transferred plugs in the medial femoral condyle region.
  
  
  CONCLUSIONS
  Our findings demonstrate the ability to successfully transfer an osteochondral autograft plug with maintenance of chondrocyte cellular viability. The transferred cartilage is stiffer than the control medial femoral condyle cartilage, and there is concern regarding the increased trabecular mass in the healing subchondral plate, but these do not result in increased degenerative changes of the opposing articular surfaces in the short term.
authors.affiliation: Department of Orthopaedics, Connective Tissue Biochemistry Laboratories, The University of California San Diego, La Jolla, California, USA.
authors.forename: J G
authors.initials: JG
authors.lastname: Lane
authors.forename: W L
authors.initials: WL
authors.lastname: Tontz
authors.forename: S T
authors.initials: ST
authors.lastname: Ball
authors.forename: J B
authors.initials: JB
authors.lastname: Massie
authors.forename: A C
authors.initials: AC
authors.lastname: Chen
authors.forename: W C
authors.initials: WC
authors.lastname: Bae
authors.forename: M E
authors.initials: ME
authors.lastname: Amiel
authors.forename: R L
authors.initials: RL
authors.lastname: Sah
authors.forename: D
authors.initials: D
authors.lastname: Amiel
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D016025:Bone Transplantation / Q000379:methods* / Q000473:pathology; D002358:Cartilage, Articular / Q000473:pathology / Q000601:surgery*; D002470:Cell Survival; D019902:Chondrocytes / Q000502:physiology / Q000637:transplantation; D005269:Femur / Q000473:pathology / Q000601:surgery*; D006025:Glycosaminoglycans / Q000378:metabolism; D006041:Goats; D007719:Knee Joint / Q000473:pathology / Q000601:surgery; D008297:Male; D018613:Microscopy, Confocal; D010027:Osteotomy / Q000379:methods; D014182:Transplantation, Autologous
chemical_list: D006025:Glycosaminoglycans