title: [Inhibitory effect of methanol extract of Boschniakia rossica Fedtsch. et Flerov on rat hepatic preneoplastic lesions induced by diethylnitrosamine].
abstract: OBJECTIVE
  To investigate the inhibitory effect of Boschniakia rossica(BR) on hepatocarcinogenesis in rats.
  
  
  METHODS
  Based on immunohistochemistry techniques, the expression of placental form glutathione S-transferase(GST-P), mutant p53 and p21 protein were investigated in hepatic preneoplastic lesions induced by Solt-Farber protocol in the liver of rats that had been treated with the above method, administered with BR extract and of control group.
  
  
  RESULTS
  The extract of BR(500 mg/kg) has inhibitory effect on the formation of diethylnitrosamine-induced GST-P-positive foci in F344 rat and the expression of mutant p53 and p21 protein was lower than that of hepatic preneoplastic lesions, and the increasing gamma-glutamyltranspeptidase(gamma-GT) activity in rat liver treated with Solt-Farber protocol was decreased by the extract of BR.
  
  
  CONCLUSIONS
  These results indicate that BR has inhibitory effect on DEN induced hepatic preneoplastic lesions in F344 rat.
authors.affiliation: Biochemistry Laboratory for Cancer Research, Yanbian University College of Medicine, Yanji 133000.
authors.forename: Z Z
authors.initials: ZZ
authors.lastname: Yin
authors.forename: H L
authors.initials: HL
authors.lastname: Jin
authors.forename: T Z
authors.initials: TZ
authors.lastname: Li
authors.forename: J J
authors.initials: JJ
authors.lastname: Lee
authors.forename: Y H
authors.initials: YH
authors.lastname: Kim
authors.forename: C H
authors.initials: CH
authors.lastname: Lee
authors.forename: K H
authors.initials: KH
authors.lastname: Lee
authors.forename: B H
authors.initials: BH
authors.lastname: Hyun
mesh_terms: D000818:Animals; D050759:Cyclin-Dependent Kinase Inhibitor p21; D016213:Cyclins / Q000378:metabolism; D004052:Diethylnitrosamine; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D005982:Glutathione Transferase / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D019684:Magnoliopsida / Q000737:chemistry*; D008297:Male; D010946:Plants, Medicinal / Q000737:chemistry*; D011230:Precancerous Conditions / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D051381:Rats; D011916:Rats, Inbred F344; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
chemical_list: C493958:Cdkn1a protein, rat; D050759:Cyclin-Dependent Kinase Inhibitor p21; D016213:Cyclins; D004365:Drugs, Chinese Herbal; D016159:Tumor Suppressor Protein p53; D004052:Diethylnitrosamine; D005982:Glutathione Transferase