Analysis of fatty acids released by crotoxin in rat brain synaptosomes.
Crotoxin, the main toxin of Crotalus durissus terrificus venom, exerts its lethal effect by blocking neurotransmission at the neuromuscular junction level through a triphasic mechanism. This effect seems to depend on its phospholipasic activity, suggesting that the mechanism of neurotransmission blockage may be related to fatty acids release in specific sites of the nervous terminal. In this work, we purified the fatty acids released by crotoxin's activity and this outline was compared with other phospholipases A(2), including CB, a subunit of crotoxin. Our results show a higher release of palmitate and arachidonate by crotoxin when compared to other phospholipases A(2). Since palmitate has a role in protein acylation processes and arachidonate participates in signal transduction events, these mechanisms may be related to the neurotoxic actions of crotoxin.
Kattah
Luciene Rodrigues
LR
Laboratório de Venenos e Toxinas Animais, Departamento de Bioquímica e Imunologia, ICB, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, 31270-901, -MG, Belo Horizonte, Brazil.
Ferraz
Vany
V
Matos Santoro
Marcelo
M
Ribeiro da Silva Camargos
Elizabeth
E
Ribeiro Diniz
Carlos
C
De Lima
Maria Elena
ME
D000818:Animals; D016718:Arachidonic Acid / Q000032:analysis; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism; D003439:Crotoxin / Q000633:toxicity*; D005227:Fatty Acids / Q000032:analysis*; D005260:Female; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D008854:Microscopy, Electron; D019308:Palmitic Acid / Q000032:analysis; D010741:Phospholipases A / Q000633:toxicity; D051381:Rats; D017208:Rats, Wistar; D013574:Synaptosomes / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure
D005227:Fatty Acids; D016718:Arachidonic Acid; D019308:Palmitic Acid; D003439:Crotoxin; D007770:L-Lactate Dehydrogenase; D010741:Phospholipases A