[Deletion of YNZ22 and ALU-VPA/MYCL1 loci in human colonic adenocarcinoma and postoperative prognosis].
Since deletions of the short arm of chromosome 17 are the most common genetic defects in human colorectal carcinoma (CC), we tested the YNZ22 locus (D17S30, 17p13.3) for loss of heterozygosity (LH) in adenocarcinoma and in the normal colonic mucosa of 49 CC patients, and studied the association of LH with clinicomorphological features of the tumor. Allele frequency distribution of YNZ22 did not differ for the patients and healthy people. LH in YNZ22 in the tumor was found in 33% (13/39) of all informative cases, its frequency being thrice higher in men than in women (chi 2 = 5.21, p = 0.022). The defect was associated with moderate or poor histological differentiation (P2 = 0.0055) and polyploidy > 3n (P2 = 0.0035) of tumor cells and with high incidence of post-surgery relapse or metastasis. Analysis of both YNZ22 and Alu-VpA/MycL1 (1p34.3) loci in the tumor allowed reliable relapse prognosis in 76% of the CC patients. The probability of post-surgery relapse or metastasis was estimated at no less than 67% for patients with LH in at least one of the two loci in the tumor, and at somewhat more than 20% for patients without LH.
Kashkin
K N
KN
kirill@online.ru
Nikolaev
A V
AV
Turbin
D A
DA
Perevoshchikov
A G
AG
D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D001483:Base Sequence; D002872:Chromosome Deletion; D003110:Colonic Neoplasms / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D017931:DNA Primers; D006801:Humans; D011379:Prognosis
D017931:DNA Primers
Deletsii lokusov YNZ22 i ALU-VPA/MYCL1 v adenokartsionomakh tolstoĭ kishki cheloveka i posleoperatsionnyĭ prognoz.