Stable transformation of Pleurotus ostreatus to hygromycin B resistance using Lentinus edodes GPD expression signals.
It was reported that Pleurotus ostreatus was transformed unstably using recombinant plasmids containing a hygromycin B phosphotransferase gene (hph) under the control of Aspergillus nidulans expression signals, and that the plasmids were maintained extrachromosomally in the transformants. Here we report a stable and integrative transformation of the fungus to hygromycin B resistance, using a recombinant hph fused with Lentinus edodes glyceraldehyde-3-phosphate dehydrogenase expression signals. Restriction-enzyme-mediated integration (REMI) was also tried and increased the transformation efficiency about ten-fold.
Irie
T
T
Wood Research Institute, Kyoto University, Uji, Japan.
Honda
Y
Y
Hirano
T
T
Sato
T
T
Enei
H
H
Watanabe
T
T
Kuwahara
M
M
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D025141:Drug Resistance, Fungal / Q000235:genetics; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics / Q000378:metabolism; D006921:Hygromycin B / Q000494:pharmacology*; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics; D020076:Pleurotus / Q000187:drug effects* / Q000235:genetics*; D011995:Recombination, Genetic; D020942:Shiitake Mushrooms / Q000201:enzymology / Q000235:genetics*; D014170:Transformation, Genetic
D000900:Anti-Bacterial Agents; D006921:Hygromycin B; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D017853:Phosphotransferases (Alcohol Group Acceptor); C063242:hygromycin-B kinase