title: Significant reduction of T-cell chemotaxis to MCP-1 in patients with primary and metastatic melanoma.
abstract: OBJECTIVE
  To address whether circulating T-cells from the patients with primary and metastatic malignant melanoma show altered chemotaxis to monocyte-chemotactic protein 1 (MCP-1) and its relation to tumor infiltrating lymphocyte (TIL) and metastasis.
  
  
  METHODS
  Chemotactic response of T-cells towards MCP-1 and immuno-histochemistry study of TIL and tumor-associated-macrophages (TAM) were investigated in patients with primary and metastatic melanoma compared to patients with basal cell carcinoma and healthy persons.
  
  
  RESULTS
  T-cells from patients with primary and metastatic melanoma showed a significantly decreased chemotactic migration towards MCP-1 and T-cells from patients with basal cell carcinoma showed normal chemotactic response. Immuno-histochemistry study showed that there was no correlation between the number of TIL and the decreased chemotaxis of circulating T-cells to MCP-1 in patients with primary melanoma.
  
  
  CONCLUSIONS
  Circulating T-cells from patients with primary and metastatic melanoma showed a MCP-1-specific decrease in chemotactic migration. This may be due to abnormal expression or modulation of MCP-1-receptor expression on these cells.
authors.affiliation: Department of Dermatology, Second Affiliated Hospital of Zhejiang Medical University, Hangzhou 310009, China.
authors.forename: M
authors.initials: M
authors.lastname: Zheng
authors.forename: G
authors.initials: G
authors.lastname: Sun
authors.forename: S
authors.initials: S
authors.lastname: Cai
authors.forename: R
authors.initials: R
authors.lastname: Mueller
authors.forename: U
authors.initials: U
authors.lastname: Mrowietz
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D018932:Chemokine CCL2 / Q000276:immunology*; D002634:Chemotaxis, Leukocyte; D005260:Female; D006801:Humans; D016131:Lymphocyte Subsets / Q000276:immunology; D016246:Lymphocytes, Tumor-Infiltrating / Q000276:immunology; D008264:Macrophages / Q000276:immunology; D008297:Male; D008545:Melanoma / Q000276:immunology* / Q000556:secondary; D008875:Middle Aged; D012878:Skin Neoplasms / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D018932:Chemokine CCL2