[Antisense oligonucleotides inhibit c-myc and PCNA expression in the vascular smooth muscle cells].
OBJECTIVE
To study the methods for prevention and treatment of vascular restenosis.


METHODS
We used two factors which result in hyperplasia of vascular smooth muscle cells (VSMCs) and applied antisense c-myc, PCNA oligonucleotides to attenuate the expression of their related gene. The sense, antisense, and mismatched oligomers for c-myc or PCNA were designed and synthesized. They were individually delivered to the cultured VSMCs after the oligomers were stable in culture medium for 5 days and could cross the plasma membranes into the cells. Using RT-PCR and the image analysis system, we observed the changes of both c-myc and PCNA gene expression in the VSMCs.


RESULTS
Antisense c-myc and PCNA at a concentration of 10 mumol/L were able to inhibit the expression of c-myc and PCNA gene in VSMCs (P < 0.05), whereas the sense and the mismatched ones couldn't inhibit the expression (P > 0.05).


CONCLUSIONS
The antisense technique is an effective method for the inhibition of VSMCs proliferation.
Bian
J
J
Department of Vascular Surgery, Renji Hospital, Shanghai Second Medical University, Shanghai 200001.
Zhang
B
B
Chen
S
S
D000818:Animals; D001013:Aorta, Thoracic / Q000166:cytology; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D015870:Gene Expression / Q000187:drug effects; D016259:Genes, myc; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000378:metabolism*; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D018809:Proliferating Cell Nuclear Antigen / Q000096:biosynthesis* / Q000235:genetics; D016271:Proto-Oncogene Proteins c-myc / Q000096:biosynthesis* / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar
D016376:Oligonucleotides, Antisense; D018809:Proliferating Cell Nuclear Antigen; D016271:Proto-Oncogene Proteins c-myc