Kinetics of membrane adhesion mediated by ligand-receptor interaction studied with a biomimetic system.
We report the first measurement of the kinetics of adhesion of a single giant vesicle controlled by the competition between membrane-substrate interaction mediated by ligand-receptor interaction, gravitation, and Helfrich repulsion. To model the cell-tissue interaction, we doped the vesicles with lipid-coupled polymers (mimicking the glycocalix) and the reconstituted ligands selectively recognized by alpha(IIb)beta(3) integrin-mediating specific attraction forces. The integrin was grafted on glass substrates to act as a target cell. The adhesion of the vesicle membrane to the integrin-covered surface starts with the spontaneous formation of a small (approximately 200 nm) domain of tight adhesion, which then gradually grows until the whole adhesion area is in the state of tight adhesion. The time of adhesion varies from few tens of seconds to about one hour depending on the ligand and lipopolymer concentration. At small ligand concentrations, we observed the displacement xi of the front of tight adhesion following the square root law xi approximately t(1/2), whereas, at high concentrations, we found a linear law xi approximately t. We show both experimentally and theoretically that the t(1/2)-regime is dominated by diffusion of ligands, and the xi approximately t-regime by the kinetics of ligands-receptors association.
Boulbitch
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A
Department für Biophysik E22, Technische Universität München, D-85747 Garching bei München, Germany. aboulbit@physik.tu-muenchen.de
Guttenberg
Z
Z
Sackmann
E
E
D020816:Amino Acid Motifs; D002448:Cell Adhesion / Q000502:physiology; D002465:Cell Movement / Q000502:physiology; D002784:Cholesterol / Q000737:chemistry; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry; D019276:Glycocalyx / Q000378:metabolism*; D006112:Gravitation; D007700:Kinetics; D008024:Ligands; D008567:Membranes, Artificial; D008954:Models, Biological; D018716:Molecular Mimicry / Q000502:physiology*; D010456:Peptides, Cyclic / Q000737:chemistry; D019039:Platelet Glycoprotein GPIIb-IIIa Complex / Q000378:metabolism*; D011092:Polyethylene Glycols / Q000737:chemistry; D011956:Receptors, Cell Surface / Q000378:metabolism*; D055672:Static Electricity; D013816:Thermodynamics
D008024:Ligands; D008567:Membranes, Artificial; D010456:Peptides, Cyclic; D019039:Platelet Glycoprotein GPIIb-IIIa Complex; D011956:Receptors, Cell Surface; D011092:Polyethylene Glycols; D002784:Cholesterol; D004134:Dimyristoylphosphatidylcholine