title: A hybrid bacterial replication origin.
abstract: We constructed a hybrid replication origin that consists of the main part of oriC from Escherichia coli, the DnaA box region and the AT-rich region from Bacillus subtilis oriC. The AT-rich region could be unwound by E. coli DnaA protein, and the DnaB helicase was loaded into the single-stranded bubble. The results show that species specificity, i.e. which DnaA protein can do the unwinding, resides within the DnaA box region of oriC.
authors.affiliation: Max-Planck-Institut für molekulare Genetik, Ihnestrasse 73, D-14195 Berlin, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Seitz
authors.forename: M
authors.initials: M
authors.lastname: Welzeck
authors.forename: W
authors.initials: W
authors.lastname: Messer
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D018983:DNA Footprinting; D004265:DNA Helicases / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics*; D053488:DnaB Helicases; D004926:Escherichia coli / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids / Q000378:metabolism; D011196:Potassium Permanganate / Q000494:pharmacology; D018741:Replication Origin; D013045:Species Specificity
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C066312:DnaA protein, Bacteria; D011196:Potassium Permanganate; D004265:DNA Helicases; D053488:DnaB Helicases