[Brain-specific sequence Hfb1 is part of the 3'-untranslated region of the human complexin 2 gene: new data from in vitro and in silico experiments].
A genomic clone hybridizing with brain-specific sequence Hfb1 was isolated from a chromosome 5 consmid library. Hfb1 proved to correspond to a new gene exon which codes for a large 3'-untranslated region of the mRNA for synaptic protein complexin 2. Together with the 985-nt Hfb1 cDNA (EMBL Y15167) isolated previously from a cDNA library of the frontal cerebral cortex, the primary structure was established for genomic clone Ghfb sized more than 4 kb. A GenBank search revealed complete identity of the 5' end of Ghfb and the 3'-untranslated region (878-933) of the human complexin 2 mRNA. Large transcripts with the 5' end corresponding to the complexin 2 mRNA and the 3' end to Ghfb were detected in total mRNA of the human brain by means of RT-PCR. The size of the 3'-untranslated region of the human complexin 2 mRNA was estimated at 4 kb.
Dergunova
L V
LV
dergunov@img.ras.ru
Raevskaia
N M
NM
Vladychenskaia
I P
IP
Poltaraus
A B
AB
Klimov
E A
EA
Udina
I G
IG
Sulimova
G E
GE
Limborskaia
S A
SA
D020413:3' Untranslated Regions; D033942:Adaptor Proteins, Vesicular Transport; D001483:Base Sequence; D001921:Brain / Q000378:metabolism*; D002874:Chromosome Mapping; D002895:Chromosomes, Human, Pair 5; D018076:DNA, Complementary; D006801:Humans; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D020413:3' Untranslated Regions; D033942:Adaptor Proteins, Vesicular Transport; D018076:DNA, Complementary; D009419:Nerve Tissue Proteins; C095993:complexin II
Mozgospetsificheskaia posledovatel'nost' Hfb1 vkhodit v sostav protiazhennoĭ 3'-netransliruemoĭ oblasti gena komplksina 2 cheloveka: novye dannye in vitro i in silico éksperimentov.