title: Structural characterization and chromosomal localization of the MAGE-E1 gene.
abstract: Genes of the melanoma-associated antigen (MAGE) family are characterized by the expression of tumor antigens on a malignant melanoma recognized by autologous cytolytic T lymphocytes. We have previously identified novel members of the MAGE gene family expressed in human glioma and named them MAGE-E1a-c. In the present study, we have revealed the genomic structure of MAGE-E1 by sequence analysis of a human chromosome bacterial artificial chromosome clone containing the MAGE-E1 gene. The MAGE-E1 gene is composed of 13 exons, and three of these (exon 2, exon 3 and exon 12) are alternatively spliced in each variant (E1a-c). The open reading frame encoding the MAGE-E1 peptides initiates in exon 2 and ends in exon 13. We have also demonstrated that the MAGE-E1 gene is located in Xp11 through the analysis of radiation hybrid panels. The genomic structure of MAGE-E1 is markedly similar to that of MAGE-D and its chromosomal locus is also identical to that of MAGE-D, but these features contrast with those of other MAGEs. These results suggest that MAGE-D and -E1 may be evolutionarily distant from other members of the MAGE family, and the two may be ancestral genes for the others.
authors.affiliation: Laboratory of Neural Information, National Institute for Physiological Sciences, Okazaki 444-8585, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Kawano
authors.forename: M
authors.initials: M
authors.lastname: Sasaki
authors.forename: K
authors.initials: K
authors.lastname: Nakahira
authors.forename: T
authors.initials: T
authors.lastname: Yoshimine
authors.forename: K
authors.initials: K
authors.lastname: Shimizu
authors.forename: H
authors.initials: H
authors.lastname: Wada
authors.forename: K
authors.initials: K
authors.lastname: Ikenaka
mesh_terms: D017398:Alternative Splicing; D000951:Antigens, Neoplasm / Q000235:genetics*; D001483:Base Sequence; D015139:Blotting, Southern; D002874:Chromosome Mapping; D022202:Chromosomes, Artificial, Bacterial; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry / Q000235:genetics; D005091:Exons; D005796:Genes / Q000235:genetics*; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics; D017422:Sequence Analysis, DNA; D024363:Transcription Initiation Site; D014960:X Chromosome / Q000235:genetics
chemical_list: D000951:Antigens, Neoplasm; C430404:MAGE-E1 antigen, human; D004247:DNA