title: Intranuclear localization of the transcription coadaptor CBP/p300 and the transcription factor RBP-Jk in relation to EBNA-2 and -5 in B lymphocytes.
abstract: We have studied the expression and the localization of the cellular proteins CBP/p300 and RBP-Jk in in vitro EBV-infected human B lymphocytes in relation to the EBNA-2 and EBNA-5 proteins. We found that the level of CBP/p300 was elevated drastically by EBV infection and also after activation by CD40 ligation. Thus the increase in CBP/p300 expression in the EBV-infected cells is related to the virus-induced activation and proliferation of the cells. EBNA-2 and RBP-Jk colocalized in the nucleoplasm, which is in accordance with their functional interaction. We confirmed earlier reports about the presence and colocalization of EBNA-5 and CBP in the nuclear POD bodies. On the other hand, neither EBNA-2 nor p300 was detected in the PODs. The expression of these two proteins overlapped in some distinct dots of the nucleoplasm. Taken together, the different patterns of CBP and p300 expression and their different localization in relation to the PML bodies and two EBV-encoded proteins in the B cells may provide some clue to their distinct functional roles.
authors.affiliation: Microbiology and Tumor Biology Center, Karolinska Institute, S-171 77 Stockholm, Sweden. kentaro.bandobashi@mtc.ki.se
authors.forename: K
authors.initials: K
authors.lastname: Bandobashi
authors.forename: A
authors.initials: A
authors.lastname: Maeda
authors.forename: N
authors.initials: N
authors.lastname: Teramoto
authors.forename: N
authors.initials: N
authors.lastname: Nagy
authors.forename: L
authors.initials: L
authors.lastname: Székely
authors.forename: H
authors.initials: H
authors.lastname: Taguchi
authors.forename: I
authors.initials: I
authors.lastname: Miyoshi
authors.forename: G
authors.initials: G
authors.lastname: Klein
authors.forename: E
authors.initials: E
authors.lastname: Klein
mesh_terms: D001402:B-Lymphocytes / Q000378:metabolism / Q000821:virology; D050882:CREB-Binding Protein; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000378:metabolism*; D019309:Epstein-Barr Virus Nuclear Antigens / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D004854:Herpesvirus 4, Human / Q000235:genetics; D006801:Humans; D051819:Immunoglobulin J Recombination Signal Sequence-Binding Protein; D009687:Nuclear Proteins / Q000096:biosynthesis / Q000378:metabolism*; D014066:Palatine Tonsil / Q000166:cytology; D015534:Trans-Activators / Q000096:biosynthesis / Q000378:metabolism*; D014157:Transcription Factors / Q000096:biosynthesis / Q000378:metabolism*; D014158:Transcription, Genetic; D014162:Transfection; D014764:Viral Proteins
chemical_list: D004268:DNA-Binding Proteins; C402641:EBNA-2 protein, Human herpesvirus 4; C421621:EBNA-5 protein, Epstein-Barr virus; D019309:Epstein-Barr Virus Nuclear Antigens; D051819:Immunoglobulin J Recombination Signal Sequence-Binding Protein; D009687:Nuclear Proteins; C497570:RBPJ protein, human; D015534:Trans-Activators; D014157:Transcription Factors; D014764:Viral Proteins; D050882:CREB-Binding Protein; C494363:CREBBP protein, human