title: Molecular distribution sensing in a fluorescence resonance energy transfer based affinity assay for glucose.
abstract: A newly developed method for determining molecular distribution functions is applied to a widely researched glucose affinity sensor. The reduction in fluorescence resonance energy transfer (FRET) to a malachite green (MG)-dextran complex from allophycocyanin (APC) bound to concanavalin A (ConA) due to displacement of the complex by glucose from ConA provides the basis of the assay. The higher sensitivity and specificity of a new approach to fluorescence decay analysis, over the methods based on conventional Forster-type models, is demonstrated and critical parameters in competitive binding FRET sensing derived.
authors.affiliation: Department of Physics and Applied Physics, University of Strathclyde, Glasgow, Scotland, UK.
authors.forename: O J
authors.initials: OJ
authors.lastname: Rolinski
authors.forename: D J
authors.initials: DJ
authors.lastname: Birch
authors.forename: L
authors.initials: L
authors.lastname: McCartney
authors.forename: J C
authors.initials: JC
authors.lastname: Pickup
mesh_terms: D003208:Concanavalin A / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D005947:Glucose / Q000032:analysis*; D007700:Kinetics; D015233:Models, Statistical; D010798:Phycocyanin / Q000737:chemistry; D012394:Rosaniline Dyes / Q000737:chemistry; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013997:Time Factors
chemical_list: D012394:Rosaniline Dyes; C002497:allophycocyanin; D010798:Phycocyanin; D003208:Concanavalin A; C005095:malachite green; D005947:Glucose